LOCUS       DAMZII010000001        81142 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZII010000001 DAMZII010000000
VERSION     DAMZII010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.001"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..157
                     /locus_tag="POA77_000001"
     CDS             <1..157
                     /locus_tag="POA77_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDI6363504.1"
                     /translation="IKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIG
                     GCGPTAQ"
     gene            complement(264..1349)
                     /locus_tag="POA77_000002"
     CDS             complement(264..1349)
                     /locus_tag="POA77_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6363505.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1361..2665)
                     /locus_tag="POA77_000003"
     CDS             complement(1361..2665)
                     /locus_tag="POA77_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDI6363506.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2677..3030)
                     /locus_tag="POA77_000004"
     CDS             complement(2677..3030)
                     /locus_tag="POA77_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDI6363507.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3041..3919)
                     /locus_tag="POA77_000005"
     CDS             complement(3041..3919)
                     /locus_tag="POA77_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDI6363508.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3916..5142)
                     /locus_tag="POA77_000006"
     CDS             complement(3916..5142)
                     /locus_tag="POA77_000006"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6363509.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5142..6305)
                     /locus_tag="POA77_000007"
     CDS             complement(5142..6305)
                     /locus_tag="POA77_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDI6363510.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6389..6751)
                     /locus_tag="POA77_000008"
     CDS             complement(6389..6751)
                     /locus_tag="POA77_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI6363511.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6768..7673)
                     /locus_tag="POA77_000009"
     CDS             complement(6768..7673)
                     /locus_tag="POA77_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363512.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7833..8837)
                     /gene="trpS"
                     /locus_tag="POA77_000010"
     CDS             complement(7833..8837)
                     /gene="trpS"
                     /locus_tag="POA77_000010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI6363513.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8830..9588)
                     /gene="gph"
                     /locus_tag="POA77_000011"
     CDS             complement(8830..9588)
                     /gene="gph"
                     /locus_tag="POA77_000011"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDI6363514.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPSVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9581..10258)
                     /gene="rpe"
                     /locus_tag="POA77_000012"
     CDS             complement(9581..10258)
                     /gene="rpe"
                     /locus_tag="POA77_000012"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6363515.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10276..11112)
                     /gene="dam"
                     /locus_tag="POA77_000013"
     CDS             complement(10276..11112)
                     /gene="dam"
                     /locus_tag="POA77_000013"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDI6363516.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11219..12505)
                     /gene="damX"
                     /locus_tag="POA77_000014"
     CDS             complement(11219..12505)
                     /gene="damX"
                     /locus_tag="POA77_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDI6363517.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12597..13685)
                     /gene="aroB"
                     /locus_tag="POA77_000015"
     CDS             complement(12597..13685)
                     /gene="aroB"
                     /locus_tag="POA77_000015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI6363518.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13742..14263)
                     /gene="aroK"
                     /locus_tag="POA77_000016"
     CDS             complement(13742..14263)
                     /gene="aroK"
                     /locus_tag="POA77_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDI6363519.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14664..15902)
                     /gene="hofQ"
                     /locus_tag="POA77_000017"
     CDS             complement(14664..15902)
                     /gene="hofQ"
                     /locus_tag="POA77_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDI6363520.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15814..16218)
                     /gene="hofP"
                     /locus_tag="POA77_000018"
     CDS             complement(15814..16218)
                     /gene="hofP"
                     /locus_tag="POA77_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDI6363521.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16208..16648)
                     /gene="hofO"
                     /locus_tag="POA77_000019"
     CDS             complement(16208..16648)
                     /gene="hofO"
                     /locus_tag="POA77_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDI6363522.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16632..17171)
                     /gene="hofN"
                     /locus_tag="POA77_000020"
     CDS             complement(16632..17171)
                     /gene="hofN"
                     /locus_tag="POA77_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDI6363523.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17171..17950)
                     /gene="hofM"
                     /locus_tag="POA77_000021"
     CDS             complement(17171..17950)
                     /gene="hofM"
                     /locus_tag="POA77_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDI6363524.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            18070..20622
                     /gene="mrcA"
                     /locus_tag="POA77_000022"
     CDS             18070..20622
                     /gene="mrcA"
                     /locus_tag="POA77_000022"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDI6363525.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20787..21347)
                     /gene="nudE"
                     /locus_tag="POA77_000023"
     CDS             complement(20787..21347)
                     /gene="nudE"
                     /locus_tag="POA77_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDI6363526.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21667..23802
                     /gene="igaA"
                     /locus_tag="POA77_000024"
     CDS             21667..23802
                     /gene="igaA"
                     /locus_tag="POA77_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDI6363527.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            23867..24535
                     /gene="yrfG"
                     /locus_tag="POA77_000025"
     CDS             23867..24535
                     /gene="yrfG"
                     /locus_tag="POA77_000025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDI6363528.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24546..24947
                     /gene="hslR"
                     /locus_tag="POA77_000026"
     CDS             24546..24947
                     /gene="hslR"
                     /locus_tag="POA77_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDI6363529.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24972..25850
                     /gene="hslO"
                     /locus_tag="POA77_000027"
     CDS             24972..25850
                     /gene="hslO"
                     /locus_tag="POA77_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI6363530.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25985..27709)
                     /locus_tag="POA77_000028"
     CDS             complement(25985..27709)
                     /locus_tag="POA77_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDI6363531.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            28088..29710
                     /gene="pckA"
                     /locus_tag="POA77_000029"
     CDS             28088..29710
                     /gene="pckA"
                     /locus_tag="POA77_000029"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI6363532.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29826..30143
                     /locus_tag="POA77_000030"
     CDS             29826..30143
                     /locus_tag="POA77_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6363533.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30202..30498
                     /locus_tag="POA77_000031"
     CDS             30202..30498
                     /locus_tag="POA77_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6363534.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKSI"
     gene            complement(30528..31880)
                     /gene="envZ"
                     /locus_tag="POA77_000032"
     CDS             complement(30528..31880)
                     /gene="envZ"
                     /locus_tag="POA77_000032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDI6363535.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTIKEG
                     "
     gene            complement(31877..32596)
                     /gene="ompR"
                     /locus_tag="POA77_000033"
     CDS             complement(31877..32596)
                     /gene="ompR"
                     /locus_tag="POA77_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDI6363536.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32824..33300
                     /gene="greB"
                     /locus_tag="POA77_000034"
     CDS             32824..33300
                     /gene="greB"
                     /locus_tag="POA77_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDI6363537.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33397..35718
                     /locus_tag="POA77_000035"
     CDS             33397..35718
                     /locus_tag="POA77_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI6363538.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMSKKR"
     gene            36156..36383
                     /gene="feoA"
                     /locus_tag="POA77_000036"
     CDS             36156..36383
                     /gene="feoA"
                     /locus_tag="POA77_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDI6363539.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36400..38721
                     /gene="feoB"
                     /locus_tag="POA77_000037"
     CDS             36400..38721
                     /gene="feoB"
                     /locus_tag="POA77_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDI6363540.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38721..38957
                     /gene="feoC"
                     /locus_tag="POA77_000038"
     CDS             38721..38957
                     /gene="feoC"
                     /locus_tag="POA77_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDI6363541.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            39160..40038
                     /gene="rpnA"
                     /locus_tag="POA77_000039"
     CDS             39160..40038
                     /gene="rpnA"
                     /locus_tag="POA77_000039"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDI6363542.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(40067..40837)
                     /gene="bioH"
                     /locus_tag="POA77_000040"
     CDS             complement(40067..40837)
                     /gene="bioH"
                     /locus_tag="POA77_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDI6363543.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            40875..41558
                     /gene="gntX"
                     /locus_tag="POA77_000041"
     CDS             40875..41558
                     /gene="gntX"
                     /locus_tag="POA77_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDI6363544.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41617..42192
                     /gene="nfuA"
                     /locus_tag="POA77_000042"
     CDS             41617..42192
                     /gene="nfuA"
                     /locus_tag="POA77_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDI6363545.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42553..43869
                     /gene="gntT"
                     /locus_tag="POA77_000043"
     CDS             42553..43869
                     /gene="gntT"
                     /locus_tag="POA77_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI6363546.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43914..45998)
                     /gene="malQ"
                     /locus_tag="POA77_000044"
     CDS             complement(43914..45998)
                     /gene="malQ"
                     /locus_tag="POA77_000044"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDI6363547.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46008..48401)
                     /gene="malP"
                     /locus_tag="POA77_000045"
     CDS             complement(46008..48401)
                     /gene="malP"
                     /locus_tag="POA77_000045"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDI6363548.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49013..51718
                     /gene="malT"
                     /locus_tag="POA77_000046"
     CDS             49013..51718
                     /gene="malT"
                     /locus_tag="POA77_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDI6363549.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(51761..52777)
                     /gene="rtcA"
                     /locus_tag="POA77_000047"
     CDS             complement(51761..52777)
                     /gene="rtcA"
                     /locus_tag="POA77_000047"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDI6363550.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(52781..54007)
                     /gene="rtcB"
                     /locus_tag="POA77_000048"
     CDS             complement(52781..54007)
                     /gene="rtcB"
                     /locus_tag="POA77_000048"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDI6363551.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54196..55794
                     /gene="rtcR"
                     /locus_tag="POA77_000049"
     CDS             54196..55794
                     /gene="rtcR"
                     /locus_tag="POA77_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDI6363552.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGTEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(55776..56534)
                     /locus_tag="POA77_000050"
     CDS             complement(55776..56534)
                     /locus_tag="POA77_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDI6363553.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56551..57381)
                     /gene="glpG"
                     /locus_tag="POA77_000051"
     CDS             complement(56551..57381)
                     /gene="glpG"
                     /locus_tag="POA77_000051"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDI6363554.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57426..57752)
                     /gene="glpE"
                     /locus_tag="POA77_000052"
     CDS             complement(57426..57752)
                     /gene="glpE"
                     /locus_tag="POA77_000052"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDI6363555.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            57942..59447
                     /gene="glpD"
                     /locus_tag="POA77_000053"
     CDS             57942..59447
                     /gene="glpD"
                     /locus_tag="POA77_000053"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDI6363556.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(59501..60106)
                     /locus_tag="POA77_000054"
     CDS             complement(59501..60106)
                     /locus_tag="POA77_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363557.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(60109..60876)
                     /locus_tag="POA77_000055"
     CDS             complement(60109..60876)
                     /locus_tag="POA77_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363558.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(60879..61589)
                     /locus_tag="POA77_000056"
     CDS             complement(60879..61589)
                     /locus_tag="POA77_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363559.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(61604..63112)
                     /locus_tag="POA77_000057"
     CDS             complement(61604..63112)
                     /locus_tag="POA77_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6363560.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIKVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(63237..65684)
                     /gene="glgP"
                     /locus_tag="POA77_000058"
     CDS             complement(63237..65684)
                     /gene="glgP"
                     /locus_tag="POA77_000058"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDI6363561.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDSQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(65703..67136)
                     /gene="glgA"
                     /locus_tag="POA77_000059"
     CDS             complement(65703..67136)
                     /gene="glgA"
                     /locus_tag="POA77_000059"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDI6363562.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(67136..68431)
                     /gene="glgC"
                     /locus_tag="POA77_000060"
     CDS             complement(67136..68431)
                     /gene="glgC"
                     /locus_tag="POA77_000060"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDI6363563.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(68449..70422)
                     /gene="glgX"
                     /locus_tag="POA77_000061"
     CDS             complement(68449..70422)
                     /gene="glgX"
                     /locus_tag="POA77_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDI6363564.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(70419..72605)
                     /gene="glgB"
                     /locus_tag="POA77_000062"
     CDS             complement(70419..72605)
                     /gene="glgB"
                     /locus_tag="POA77_000062"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDI6363565.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(72878..73981)
                     /gene="asd"
                     /locus_tag="POA77_000063"
     CDS             complement(72878..73981)
                     /gene="asd"
                     /locus_tag="POA77_000063"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6363566.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLSGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            74174..74767
                     /gene="yhgN"
                     /locus_tag="POA77_000064"
     CDS             74174..74767
                     /gene="yhgN"
                     /locus_tag="POA77_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDI6363567.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(74824..76164)
                     /gene="gntU"
                     /locus_tag="POA77_000065"
     CDS             complement(74824..76164)
                     /gene="gntU"
                     /locus_tag="POA77_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI6363568.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76168..76695)
                     /gene="gntK"
                     /locus_tag="POA77_000066"
     CDS             complement(76168..76695)
                     /gene="gntK"
                     /locus_tag="POA77_000066"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6363569.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76834..77829)
                     /gene="gntR"
                     /locus_tag="POA77_000067"
     CDS             complement(76834..77829)
                     /gene="gntR"
                     /locus_tag="POA77_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDI6363570.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78053..78748)
                     /gene="yhhW"
                     /locus_tag="POA77_000068"
     CDS             complement(78053..78748)
                     /gene="yhhW"
                     /locus_tag="POA77_000068"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDI6363571.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(78871..79908)
                     /locus_tag="POA77_000069"
     CDS             complement(78871..79908)
                     /locus_tag="POA77_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6363572.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            80241..80729
                     /gene="yhhY"
                     /locus_tag="POA77_000070"
     CDS             80241..80729
                     /gene="yhhY"
                     /locus_tag="POA77_000070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6363573.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            80966..>81142
                     /locus_tag="POA77_000071"
     CDS             80966..>81142
                     /locus_tag="POA77_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDI6363574.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHG"
BASE COUNT        19732 a        22065 c        20261 g        19084 t
ORIGIN      
        1 tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta ctggtcatat
       61 tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccggcaa tttctatttc
      121 taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa atcggctaac
      181 accggtttaa ttgcccgacg ccctctggat tttccgaggg cgtatttgtt tctgcattct
      241 tttcgaaaac atcattcaca agattaaata ctggcaatac tctcacgcaa aatccgcagc
      301 accgtctctt caccgctgcg attcgggtta atgcgaatcg cacaatgttc tgattgtgga
      361 ttcgcctggc gaaacgttcc ggaaaggcga taaaagagcg gcgggatttc atatttcgac
      421 tctgcaccca ccgggtaagg caaggcaccg cgcttttgcg cctcttccag cactctggcg
      481 gcaatcggct gatgaaactc gacaatcaac accttcgact gcgcattagc aatcaccgcg
      541 cttttcactt ccggcaccgc accaccgtta agcaaagcca gcaaccgttc agatacccct
      601 gcctgcaccg cgtgcatcac tggcgcaaac accagaccac gcaatacttc cagcgcctgt
      661 gcgccctgga tctggctacc gccggagtaa agcgtggcgc gaatacggtt gataacatca
      721 gcatcgccga ccactgcacc aacaccctct ggcccaaata gcttgaagca ggaaaatgtc
      781 gagacattcg cgccgcattc acagccgatt cgcgccacct tcatcaccgc atagttgtca
      841 tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca gcacatctgc cagcacgtag
      901 ctgtcctgcg gctgctggcg cgtatgctgc accagcgccg catccggttg ttgctcgtcg
      961 acgacctgct tcagtgccga caggtcattg aaatcaacag taataagcgt cagccccatc
     1021 tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt catgcaccag aagacgctgc
     1081 cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc cagtccccgc gccctgcacc
     1141 agcgccgcag cctgtgcgtg aaatgcatca gccagcacct gttccacacg ctgggtaacg
     1201 cgcggttgat tcagtcctgg cgttaagcct aaatcaccgc cagtaagaaa ctcgctgccg
     1261 gggaaatggc gacaaatgct atccaccagc gcaaactgct tttgctgcgc ctcaataatc
     1321 gtcaggcttt gcagaggaaa cgtcttcata ggtgccttcc ttaagccgga acgaacaggc
     1381 caagccagta aagaacattc agcagaatac cggtgatcat tactgccacg accggtgccg
     1441 ccattttctg taccggacgg cccagggatt cgttgaggaa gtaaatggca accgcgatag
     1501 agaatccggt ataacccgcc atcttaattg ctgcgaaaat cgaaccgacc agcagcgcca
     1561 cttccatcag catattcatg gcgttacgga tgttatccga cgcattacgc accgacggat
     1621 agcgccccag ccatttgccg atcgaacgaa gcaataaaac ttctgccgaa ataaccactg
     1681 cacctaatac cgctgcaacc atcggattcg gcgagagata gcccaccgca taaacaaagg
     1741 taaagcccgc aactgcatac acacccgttg ctaacgcggt ggtggcaatc aacggcacaa
     1801 accccagtcc gcgcataaat tctgccagag ccgcctgatt aatcagcgtt tgcgattgtt
     1861 ccggcgttac gcctgcggaa tatgctttct ccagtgtgaa gatcgacact tcactgccag
     1921 caaaaatctt catgctggca acggcggcaa tcaatgctcc cacgatggcg atatagggta
     1981 agtttttgat aatccgtgac gtgcgttctt caaacaccga aagcccgctg gcatcaatgt
     2041 cattttcatc acgatgacgc aggtcgtgag ttatcgcgat ccccagcagc atcaccatgc
     2101 caataaagat ttcgatagat tcagggttaa gatgtgggaa atagcgcacg actaccacac
     2161 gggtcatcag taccaccacg gcggcgatca gactttgctt ccagccaaac tggtagaaaa
     2221 tcgccaccag cgggaacagt gcaaaagctg aaaccaccgg cgagcttaat tcccccaggc
     2281 tacctaatac atccaccggc agcgcggtca gcagctggtt tactggcaac aggcaagtaa
     2341 ggatcaacac gccccagata gcgccaagac caaacgccat caggctgttt atcgccagga
     2401 cgccgaggat atcggtagga agaaagagta accatgcgtt gagcagcccg gttttcaggg
     2461 taaaagagat ccccaccgag gccacaaaac caatgctcag accaaaagcg atactccccg
     2521 cctcgcgacg gttcatatag ccttcaatca gttgcggcag gatcgggcgg atgccgtcat
     2581 gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat acccgtcagg cacgccacca
     2641 cgataatctg aatatacaga tccatagtcg tgccttttat ttcagatggt taatcagcat
     2701 cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac gccacttttc cttcagcgat
     2761 cattttggcg atatgctcgt ctttcgcttt aatgcctggt ttggcaatgg tgcaactttt
     2821 gttatagcca atcaccgcga tggcaatcga caatgccgcg cccgcaccgg tattacacgc
     2881 accgatgtag taatccagtt gcccggattt cactttcatt gccgcttcca tgtcgttgtg
     2941 aatgaaaact tcaaaactgc caggagccgt cgcttcaatg gtttttttaa tctgctcacg
     3001 ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc ttattggaaa aattgagagg
     3061 gagtttcacg taacatcaca tccacatcgg cctgactgaa tcctgactgg cgcaattgcg
     3121 gaataaaggt ggtcaataaa tagtcatagc cataaccacc attggctttt aaatgggagc
     3181 ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa cccacggtca cgtagcgcat
     3241 gaagcatcgc aatacgcttt tcgtccgggt agtaactgtt cttaccaatg gtgtcgaact
     3301 gcacgtacgc gccgagatcg atcatcttca aaatgttgtc gaggttgtct ttcagatcgc
     3361 agtgaccaac ggtgacgcgc gaaagatcaa ccccgtgggc ttgtagcaac gccagttgct
     3421 ccagccccat cgtgctgaac gacgtatgcg tggagatcgg gcgtccggtc tggttatgcg
     3481 ccagcgcagc ggcaataaat accttctctt ccagcggcgt aatctttcct tcgctggtgc
     3541 cgatctccgc gatgatcccg gctttcagct ccgtgccatc gataccctgt tcaatttcat
     3601 cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac cacatgttcc gggaaaaacg
     3661 cgtcctggta ataaccggta caggccacca cgttgatccc cgtctcgcgc attacatcaa
     3721 gcataaattg cgcattgcgc cccatgtaac ggttggtcat ctcaatcaca ttacgcacgc
     3781 cccgggtcat caggtcgttc atctcctggc aaatgaacgc atactgatca aggcggcagt
     3841 ccacgttgtt tttaaagccg gagagatcaa tatgcagatg ctcatgggcg agggtgtaac
     3901 ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg agggaggaaa gaaaagagcg
     3961 accattttgt ggcggtggcg cgcggaaaaa ttcacacacg gtagccccca catcagaaag
     4021 cgtggtgcgc acaccgagct gcgtagcgat catcccttgc tgataaacca gcactggcac
     4081 cacttcgcgg gtatggtggc tgtgaccaat ggtcggatcg ttgccgtgat ccgccatcac
     4141 gaccaggcaa tcatctggct gcatcgcctc aacaagccgg gcaaggttac ggtcaacgac
     4201 ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga ccagcgaggt cggtttcctg
     4261 aatgttggtg caaataaacg ccgtcggatg ggtgttaaat tcgttgaggg tgatatccat
     4321 aatccgctgg ctatccacca gattttgcca gctcacgcca taaggattgt tgacgatatc
     4381 tgccacctta cccaccagca cggttggcac gcctgcttca tacagttttt gtggcacctg
     4441 cactttttta tcgacgccat agcccatatg cacgacctgg aaaccgttgt cataagcgcc
     4501 agaacgcggc gcattgatac caataaagcg cccttctttg ctttctgcgg catcgagaat
     4561 gcgttgactg tcggttaaca ggccaccaaa tgtaatgacc cgaccgacct gtacctgctc
     4621 acgcacgata cgaccaattt tgattgcgtc gtcaaaagag atcacagaga gattggcggt
     4681 aatgttatag acctggccta aatccgcctc gagattatcg ccaatcgcaa ccgcctgatt
     4741 gacccacaga aattgcagat catcgccacg gcgctccacc tgccagccag cggaaactaa
     4801 tgcctgctca acacggccaa tcacatcgcg aaagggcatc cgcagcggcg gtaacgggcg
     4861 cgtgcctaaa atttcctgat gccccataaa ggtatcgcca ccttcatgtt gcagctctgc
     4921 caccccccag gttgcggaat ctgacggttg catatcacct ggtgcataac ccaatgcgtt
     4981 gattagcccc agcttctcca gcgctggtag ctgcaaatgc ggcaactggc tcaggatgtg
     5041 accacaggta ttcgccccgg catcttgcgg gcgcaccagc gtgacatctt tcattgcccc
     5101 tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc attacgcccc cagagagttt
     5161 ccaagactgt cataacgacc gacgatttct ggttcgccac ggtgaatacc ggacaccagc
     5221 accacatcgc tacgagtgac aaaaatctgc gtgcggaaac agagcaccac tgcactgctc
     5281 accggatact cgcctgccag cggcagggtg tagtcgatac tgctgtcatc cacagttttg
     5341 agattggttt cagtaatctt ttgattttct ggcgtaaaca ccagcgcatg ttgcgcatga
     5401 ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg atgggagatt
     5461 tcacttaacc agagcatcgc gatacgttca ggctgatcgc cctgctggtt tgccggaata
     5521 gtgcccgtca gcgcatgacc gggttcggca tgagtcacac cgtattccgc cagcaatggc
     5581 agcgaagtgc agctggtcgc tgaaggcgcg ttcagttgct caagtgcaat accagatttc
     5641 gccagttgat cccgtgcctg tatcagcgtg tgaagattcg gtgtcggcaa aacttttccg
     5701 gcagcctcat cccaaagcag gcaagggaaa tgggtaagtc cggctaaatg cagccccggc
     5761 agtttcttaa tttcagcaac cacctcgtgc agcgaatgct gtacaaaacc gctctcctgc
     5821 cccggataaa gaaaatcatc gtcgctataa actttgagca gcacaaactg cacacgtccg
     5881 gccttcaccg ctgccgcaga aacttcccgc gctttgtcga gagtaaacac ggtgatgacg
     5941 tcggtgccct gttcaacggc gtcagcaacc tgatgacaag ggatttgtac cagatgcccc
     6001 tgatgcgcca ccgacaaacc agcgcggcgc atgactcgcg cctctttgta atccaccgcc
     6061 acaatgccgc tgtaacctaa tgccagcaat ttttccgcca gccatgggtt acggccaaac
     6121 tgcttggtca tcagatacag ttcaatgccg taaagccgcg ccgtctcaat cagtcgctta
     6181 ccgttttcca gtacctgatc cacgtcgatc acccagctgt ccggggcgat cttgccctgc
     6241 tgccacaaac ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa tgcttctaca
     6301 aacatcttgt tgcctcgttt ctgttccttg aattaaatat tcattttttt gaatatttag
     6361 gttgtgcgac gtggatttgt cgggagcttc aaacctcttc gttagcagcc atccataatc
     6421 cataaaggtt ggccagcaaa tagccttctt cacacgggtt aacttccagt gcgaattcct
     6481 tcaacaacac ctgatgcaat tgcacaacgg cttgccagtg gctggattgc gccagctctg
     6541 ccagcaattc gttatccagc ggctctattt cttcaccacg gcgactgcgc atcagtgcac
     6601 tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg caccggcaga tggcactctg
     6661 tttccagtac gttgacgacc tgcatcatgc ctttgcagat gtccttatca ataacgcctg
     6721 cctcgcaaag caggttgagt ctggtttcca tatcagatgc ctttcgttta ataactcggt
     6781 tcctgttccc cactcactac ccgttgttga tgggcgagca gggcgtgttt atctacaacg
     6841 gcacgcagca gttgttgcat tgggtaatca tcgactcgcg tcaggaccac ggcttcggtg
     6901 gcctgtaaaa atcgcggatc gtctgtgagc ggcgtcgcct ctaatcccag catggtcagt
     6961 tcgttttccg ccaccacgtt ccagatcacc gcatcgacat cgcctttaac aatgcgttgt
     7021 aaactctcgt gataagagag atcgactcgt tccacatcac taccgccaaa aaaaacatcg
     7081 gtcatgattt tctgatccgc cgaacggtta tccagcccca cgcgcttcac gtttgcggac
     7141 tcgcctttac ggcaaatcaa ctggtgctcg ccaacgtagg tgtgcggccc caactccagc
     7201 gcgaggcaaa accctttttg cgtgagataa ctttccgccg ccagtcggga aaccaccgcc
     7261 atgtcataca cgccgttaag taaacactcc acgcgaatat ccgcgccgcg catatgcgca
     7321 tagtaaaaag gaatgccatc aaactgggct ttcaatccgc tcgctaaacc ttcgtacaag
     7381 cgggtatagg gcaaaggcat cgcacatacc acgttgttga tatccacatg actcagcaat
     7441 gctttgttat ccatctcgac cagataactg ccattgcgcc cacggcgttc aatccgtatc
     7501 gctccgcttg attccagcgt tttcaacgcg gcctgcgtca ggccaacgga tgaacggcat
     7561 tcagttgcca gctcatctat ggttttcaac ctattaccgc atttttcacc caacaaataa
     7621 cgggccagcg tcgtgataac gacgccttct tttttgataa acgttcgacg cataatatat
     7681 tttcaataaa atgaatgttt atatcttcat taaaatgaag ataagtggca actgtgagat
     7741 gtggaaacgg cgaggcgctt cacatttttt cggattattg caaatgagga taaaaaaacc
     7801 gggtttcccc ggtttttggc attaatgtta aattacggct tcgccacaaa accaatcgct
     7861 tcgtataccg cttttagcgt acgggaagcg tgcgcgctgg ctttttccgc gccatctttc
     7921 atcacttgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc ctgcaattca
     7981 gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata catcttgcct
     8041 tcgaactgtt tttccagttc cgggatgctc tggcccgtta ccgcagaaag gatatccagc
     8101 aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag
     8161 tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag caggccgata
     8221 acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt
     8281 acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc gccatacagc
     8341 gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg gtcttcaccc
     8401 accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca
     8461 aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgggtcatg
     8521 cggctcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttct
     8581 ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac
     8641 aaggccagcg tatccagcgt cgctttacgc agcttctgtg catcctggcg cacggtgatc
     8701 gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga
     8761 cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa
     8821 acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg
     8881 cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc
     8941 gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg ccgcctgaat
     9001 atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca tccgctcagc
     9061 caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc cgccgataac
     9121 cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg
     9181 tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc
     9241 aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ctatagtagc gatcgaacag
     9301 tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg acggtttacc
     9361 cattgtttta cgcagagtcg cacgttcctg acgcgcccag gccaatgcac gctccatcag
     9421 aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggtaactc
     9481 caacgcatac agcgccatat ctaccgcagc agcaagaccg ggagcactgt ccaccagcgt
     9541 accgtcgaga tcaaaagcga cgccgcgaat atcttcaaac ttactcatga cttacctttg
     9601 ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg aagattgccg
     9661 aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca
     9721 cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacgg cgacgtactt
     9781 cgcgcagttt atccagtgtt tgaggaatga aagactgacc gccgaaacca ggattgacgg
     9841 acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc agaggtgtcg
     9901 ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc agcgtgcggt
     9961 caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat
    10021 caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg gcggtaatgc
    10081 catagttacg caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt
    10141 tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt
    10201 cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc
    10261 gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag
    10321 cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac
    10381 gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat
    10441 cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg
    10501 ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc
    10561 ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgcgccatgc tatcggcgta
    10621 agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc
    10681 cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc gcagattgta
    10741 acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa
    10801 cggatcctgg cttttgttga actcttcgcg gaactgatag tagacctcgg cgcaatttgt
    10861 ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat
    10921 gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt
    10981 gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa
    11041 atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg
    11101 atttttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca
    11161 gcatccagcg cttcaggcaa ctcccgcaac gataacccat tgcgggagat aaccttgatt
    11221 acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctgaacat
    11281 ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa
    11341 ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat
    11401 tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc agctgcagag
    11461 tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag
    11521 cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg
    11581 tctcttttgg tgctggcgca gaagtcgcag ccggtgcctt cgtgctcgcc acaggagcag
    11641 ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ttccgttttc acggttgctt
    11701 gcggtttttt cggttcaatc accgcctgct gacgagctgg acgcgtagtg gccggacgtt
    11761 cagcagtttg cgttttcgcc gtgtcacgcg atgcattgcc attgcgtact ggcgcgacag
    11821 ttgctggttc agtcggcaat gtagaattga ccgccacatt gttcaactgt tgctgatttt
    11881 gtggctgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg
    11941 ttgccgccgg ggtttgccct tgagtcggcg tagaagagat cggtggcaga gaaacatcct
    12001 gctgagtatt ttccgcagac gtggttcccg gcgctggctg caccccattc gcctgatcgg
    12061 tcgcattgcc tgcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagagg
    12121 tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc
    12181 cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac
    12241 gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt
    12301 cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag
    12361 tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag
    12421 aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct
    12481 cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt
    12541 tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac
    12601 gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact
    12661 tcactcttac caattgccaa cggaagaatt aagcgtatct ctcccgcaag gactttcttg
    12721 tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt
    12781 aacccagccc gcttgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg
    12841 agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac
    12901 caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt
    12961 aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa
    13021 caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc
    13081 tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc
    13141 agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact
    13201 gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt
    13261 ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga
    13321 acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca
    13381 agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta
    13441 tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta
    13501 acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg
    13561 ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta
    13621 aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc
    13681 tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga
    13741 attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat
    13801 cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg
    13861 cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct
    13921 gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg
    13981 aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac
    14041 cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc
    14101 cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg
    14161 aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg
    14221 cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa
    14281 gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa
    14341 cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc
    14401 aactgaataa atcacccatc cctctgcata tctggtcgct gcaagcgcgc tgccttgcta
    14461 ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg
    14521 cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg
    14581 gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcataaa
    14641 aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc
    14701 actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga
    14761 atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg
    14821 cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca
    14881 atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg
    14941 tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg
    15001 acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga
    15061 atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg
    15121 gcgataatat ccagctgctg tttttgttcg agcgcggaaa gctcaagatc cagcaagcgt
    15181 ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg
    15241 ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat
    15301 ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca
    15361 acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt
    15421 ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca
    15481 ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagggttata
    15541 ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctggcgggcg
    15601 atattgttat tctgccaggc aatggaatgc actgagagaa tgttgtcttc ctgccgcgtt
    15661 atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga
    15721 tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt ctcctgttca
    15781 gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc
    15841 ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc
    15901 atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggtacc
    15961 cagcgttagt acgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt
    16021 ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc
    16081 tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc
    16141 cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
    16201 ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga tcatcacctt
    16261 ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
    16321 acggcactgc ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg gatggatgcc
    16381 aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc agcgtttttt
    16441 cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc tggtgatgac
    16501 ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgac agaaaaatga
    16561 gcgtaactaa catcagcaac cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg
    16621 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
    16681 tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagaagca
    16741 tcctggcgaa gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt aagccccttg
    16801 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgttc aggtaaaaga
    16861 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt
    16921 tgcaaacgct gctcacgaat ttgttggcgc tccagcaaac gtggtttcgt tatctgtaag
    16981 ctgtgggcga gttgttgttc cccctgaagc aaaacggcgt ctacgcgagc ttcggcgctg
    17041 cttgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
    17101 agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
    17161 ggcaggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
    17221 ctggcggcgg tagcggcggc tgacgaacag aaacggcctc ccagggatca aatccgcctt
    17281 cgccacatat cgcgacgctg tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
    17341 tagtcatccc taccgccagt ttgcgccccc agcgatagcg tgtcgcccac agccactgtt
    17401 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aattgctgta
    17461 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagcgtaa
    17521 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt
    17581 cttcgctata atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc
    17641 ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc
    17701 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    17761 gactccaagg taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga
    17821 taatgtcgtt ctccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    17881 gtacgatcgc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    17941 taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    18001 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    18061 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    18121 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg
    18181 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    18241 attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag
    18301 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    18361 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    18421 gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga acgcacgctg
    18481 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac
    18541 gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt
    18601 gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggct
    18661 gtgatcgccg ggctgccgaa agcgccttcc acctttaatc cgctctactc aatggatcgt
    18721 gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccaa
    18781 caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt
    18841 gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    18901 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    18961 gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    19021 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc
    19081 gatacgctga aggcgctgcc aacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    19141 aatcctcagc aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    19201 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc aaggaccgac gccgcgtaaa
    19261 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    19321 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt
    19381 gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    19441 caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    19501 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    19561 gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta
    19621 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc
    19681 gtcgactatg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    19741 accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac
    19801 gcggtcatgg cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac
    19861 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt
    19921 ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    19981 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    20041 aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    20101 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg
    20161 cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    20221 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    20281 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    20341 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    20401 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg
    20461 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    20521 cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    20581 acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc
    20641 gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat
    20701 ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa ccccataagc ctgataagcg
    20761 ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc
    20821 acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc
    20881 atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc
    20941 gattccgggt agagatcttg cgcgaccacg atattcattt tgctggaaaa gtaagacggt
    21001 gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt
    21061 agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc tttcgaaaaa
    21121 cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc
    21181 acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc
    21241 cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg ggaacgggct
    21301 acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg
    21361 ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg
    21421 ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag
    21481 gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc
    21541 ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc
    21601 gcagaatcat gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg
    21661 gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg
    21721 ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg
    21781 gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag
    21841 agcctgacgc aggtattaca ggtgcctggc ccaacgggag ctagcgcggc accgatctct
    21901 ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac
    21961 ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac
    22021 ctgccccctt tctgggaaca atacatcaac gatgagaata ccgttgaact gatttatacc
    22081 gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa
    22141 acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag
    22201 agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt
    22261 cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc
    22321 tgcctgatta ccccggatgt atttgttccg tggctggcag gcggcgcatt actgctgctg
    22381 ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg agaaattcat
    22441 tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc
    22501 aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt
    22561 gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt
    22621 caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg
    22681 cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc
    22741 ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa
    22801 gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt
    22861 agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca
    22921 ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc attaccatta
    22981 ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg
    23041 caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa
    23101 tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc ggatttatgt
    23161 tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa
    23221 gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta
    23281 ttacgcccgg tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt
    23341 atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc
    23401 ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt
    23461 tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg
    23521 catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg
    23581 cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc
    23641 acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca ggcctggcgt
    23701 cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga aagctgcctg
    23761 aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt
    23821 tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat
    23881 tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc
    23941 cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt
    24001 tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct
    24061 aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac
    24121 caccgagatg ggaccgcgcg ccgtactgcg tgaagatacc attccgtttc ttgaggcact
    24181 gaaagccagc ggtaagcagc gaattttgct caccaatgct catccgcaca acctggcggt
    24241 aaaacttgag catacgggtc tggacgcaca ccttgattta ttactttcca cccacacatt
    24301 tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa
    24361 agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt
    24421 tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg agaaacagta
    24481 tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag
    24541 acgccatgaa agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc
    24601 gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca
    24661 acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc
    24721 agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca
    24781 gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg
    24841 cgctggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa
    24901 aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa
    24961 gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg
    25021 tgcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt
    25081 atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg
    25141 ctacgctgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc
    25201 tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg
    25261 aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca
    25321 ttaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt gataccctgg
    25381 cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc
    25441 gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg
    25501 cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa
    25561 ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag
    25621 aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt
    25681 gcgccgacgc gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg gcggaagatg
    25741 gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata
    25801 ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc
    25861 cggcagagag ggctctgccg gattttacga cgtaatggcc tgatgtgctg cgcttaccag
    25921 gcctgtgaaa taaatcgcaa attattgttg gtcagataca gcgtcgtatc cgttaaaaga
    25981 gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga tcgcgccacg
    26041 ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    26101 tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc
    26161 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    26221 tattcagatc ttgctcgacc aggacgcagg cgtctttttc aatacaggtc atcacgttat
    26281 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa
    26341 ttaacacatt atcggctaat actttttccg atacctgttc ctgaagatgt tgctctccat
    26401 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt
    26461 tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcata tagagactta
    26521 cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atggctgttc acgctaatgc
    26581 gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa
    26641 ccgcaaggtt cactttgccc tgaagaacaa cgggattttg ccctttacgc cagacgatgc
    26701 tgacaaaata ccccagcgac cacaccagta acaccagcac cgccagcacg ccctgcaagc
    26761 ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg gcgacgaaca
    26821 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc cctgtccagg
    26881 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    26941 gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg
    27001 gcagagtgat gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    27061 ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgttcgc gcgaggatca
    27121 ccgccagcgc ggtaaccaga cctaacgtga gataaataaa ccagtcggtt gcaaaaaaga
    27181 gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc actccacagt aaaagcacca
    27241 gccacgtcaa gccattggcg agaaaaatca ccagtaatat aagtacgtta tgccatactg
    27301 actggtaaaa atagcgatag cggctactgt cattgcggat gcgaaggctt tgttgtatcc
    27361 acggtagcaa caacattgcc atcaacagca gatagcagcc aaaatcccaa atcgcctttt
    27421 cagctctcca ggggttcatg ccatcagttt gccacttcaa ccagccgctc atccccagcg
    27481 tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa gagatcacgg
    27541 agaagagtaa aacggatgaa aacgcaacgg tcgcaggcac gccataaaca atccaactgt
    27601 gattttttcc ggcaagccag gcgatcaaca ggtagcaaac cagcccctga agtaatccgg
    27661 tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg ttggttatcc
    27721 agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag tattttgctt ttttcagaaa
    27781 ataatcaaaa aaagttagcg tggtgaatcg atactttacc gattgaattt gcatcaattt
    27841 cattaaggaa tgcgattcca ctcacaatat tcccgccata taaaccaaga tttaaccttt
    27901 tgagaacatt ttccacacct aaaatgctat ttctgcgata atagcaaccg tttcgtgaca
    27961 ggaatcacgg agttttttgt caaatatgaa tttctccaga tacgtaaatc tatgagcctt
    28021 gtcgcggtta acacccccaa aaagacttta ctattcaggc aatacatatt ggctaaggag
    28081 cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt atggtatcag
    28141 tgacgtacat gatatcgttt acaacccaag ctacgacctg ctgtatcagg aagagctcga
    28201 tccgagcctg acaggttatg agcgcggggt gttaactaat ctgggtgccg ttgccgtcga
    28261 taccgggatc ttcaccggtc gttcaccaaa agataagtat atcgtccgtg acgataccac
    28321 tcgcgatact ttctggtggg cagacaaagg caaaggtaaa aacgacaaca aacctctctc
    28381 tccggaaacc tggcagcatc tgaaaggcct ggtgaccagg cagctttccg gcaaacgtct
    28441 gttcgttgtc gacgctttct gtggtgcgaa cccggatact cgtctttccg tccgtttcat
    28501 caccgaagtg gcctggcagg cgcattttgt caaaaacatg tttattcgcc cgagcgatga
    28561 agagctggca ggtttcaaac cagactttat cgttatgaac ggcgcgaagt gcactaaccc
    28621 gcagtggaaa gaacagggtc tgaactccga aaacttcgtg gcgtttaacc tgaccgagcg
    28681 catgcagctg attggcggca cctggtacgg cggcgaaatg aagaaaggga tgttctcgat
    28741 gatgaactac ctgctgccgc tgaaaggtat cgcttctatg cactgctccg ccaacgttgg
    28801 tgagaaaggc gatgttgcgg tgttcttcgg cctttccggt actggtaaaa ccaccctttc
    28861 caccgacccg aaacgtcgcc tgattggcga tgacgaacac ggctgggacg atgacggtgt
    28921 gtttaacttc gaaggcggct gctacgcgaa aaccatcaag ctgtcgaaag aagcggaacc
    28981 tgaaatctac aacgctatcc gtcgtgatgc gttgctggaa aacgtcaccg tgcgtgaaga
    29041 tggcactatc gactttgatg atggttcaaa aaccgagaac acccgcgttt cttatccgat
    29101 ctatcacatc gataacatcg ttaagccggt ttcaaaagcg ggtcacgcaa cgaaagttat
    29161 cttcctgacc gcagacgcct ttggtgtgct gccaccggtt tctcgtctga cggccgatca
    29221 aactcagtac cacttcctct ctggcttcac cgccaaactg gccgggactg agcgtggcat
    29281 caccgaaccg actccaacct tctccgcctg cttcggcgcg gcgttcctgt cgctgcaccc
    29341 aactcagtac gcagaagtgc tggtaaaacg tatgcaggca gcgggcgcgc aggcgtatct
    29401 ggttaacact ggctggaacg gcactggcaa acgtatctcg attaaagata cccgcgctat
    29461 tatcgacgcc atcctcaacg gttcactgga taatgcagaa accttcactt tgccgatgtt
    29521 taacctggcg atcccaaccg aactgccggg cgtagacacc aggatccttg atccgcgtaa
    29581 cacatatgct tccccggaac agtggcagga aaaagctgaa acgctggcga aactgtttat
    29641 cgacaacttc gataaataca ctgacacccc tgcgggtgcc gcgctggtag cggctggtcc
    29701 gaagctgtaa tcagctgata tctcattgac cccttatcca aacggataag ggttttttta
    29761 tatctgatta gctaccaata tgtacacggt acacttataa ttatcaatag accgcaggat
    29821 gcattatgtt cacctttatt gaactacaag gatttagcaa acgccgccag ttattgctac
    29881 ctgatgatga gttccgggct tttcaggaac tactcattca agatcccact gccggtgaca
    29941 ttatcgcagg cacaggcagt tttagaaaaa ttcgctggaa ccgttctgga atggggaaac
    30001 ggggtggcat tcggataatt tattacaacg taacacgcaa tggccgaatt tatctggcgt
    30061 tgatttatcc taaaaatgaa caggatgatt taacagaaga acaaagaaag gcattgaaac
    30121 tattgtccga gaagctgctt taatgttgaa gctccacaaa ttccaccatg gccgaaatgc
    30181 caacggaggc attgaaaaca gatgaaagat gaattatttg ctgatcttct tgccagcgca
    30241 gaagaaatgg tacgcatcga aaagggtgag gaaacgcctg aaccagagca cgtccatact
    30301 tttagcgaaa tcgatgtaaa agcgatccgt gaagcaacgg gtttacgtca gcaagatttt
    30361 gctgtcgccg taggggtaag ttatgacctg gtcaaaagct gggaaaccaa acgccgtcaa
    30421 cctaccggag cacctcgaaa attgctcctg cttttgcaag cgaacccttt cattatcaat
    30481 caattgaaat caatataaaa caggaggcac cttcgcctcc cgtttattta cccttctttt
    30541 atcgtgccct gcgcccgcgt taccggaaca ggcagccagg cacgaatgga aagcccgccc
    30601 cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatgcg ctgcacaatt
    30661 gccagcccta gccccgtgcc gctaatggtg cgcgcactgt cgccgcggac aaatggctgg
    30721 aacaggtgct tacgctgttc cggtgcaatt cccggaccgt catcttccac ctggaaccag
    30781 gcgcgattcg gctccgttcc gctgctgact ttgatccagc cattgccata acgggcggcg
    30841 ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg
    30901 ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaataacc
    30961 tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgccc ggtgcgcagg
    31021 tagtcgataa actgctcaat gatggcgttg cactcttcga tatctttatt gatcgattct
    31081 gccagatagc catcctgctc gctcatcatc tcagtcgcca ggcgaatacg cgtcagcggc
    31141 gtgcgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta
    31201 acaccagccg ccatatggtt aaaggcacgg gtaacggaac gcacctccga agcgccatac
    31261 tcacgcagcg gcggcggaat aatcccttta ccaacctgca aggctgcgtg ttcgagatcg
    31321 accaacggtc ggttctggat acgaataaac agccacgccc cgcctatcgc caatagcata
    31381 atcgccagcg tatagcggaa cagcggagag aaatcgccct gatgaatttc ggtcagcggc
    31441 acgcgtaccc agatattggg cgacagccag gttttcagcc agacgacagg cgaacttttg
    31501 ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gtgccatctg atggcttaag
    31561 aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcctcgtt ggagtagaga
    31621 gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac caactgcgtg
    31681 ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcgtacgc gaggacttta
    31741 ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata agtcgtcacc
    31801 aggctggcga acagcaaggt gacgatgagc aataacgtac gggcaaatga acttcgtggc
    31861 gagaagcgca atcgcctcat gctttagagc cgtccggtac aaagacgtag cccaggcccc
    31921 aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc taccatgcgg cgcagacgcg
    31981 aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg gcaaggttca
    32041 tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc agtaccgcaa
    32101 actcaccgct ggtgagcggc atcggctcat cttcgcggaa catttcgcgc gtaccgaggt
    32161 taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg cctggcagtt
    32221 cgttcgcctg acgacgcagc accgcacgaa tacgggccag cagttcacgc gggttaaacg
    32281 gttttggaat gtagtcgtca gcgccaatct ccaggcctac gatacggtcc acttcttccc
    32341 ctttcgccgt caccataatg atcggcatcg ggttgctctg actacgaaga cgtcggcaaa
    32401 tcgacaagcc atcttcacca ggtaacatta aatccagtac cataagatgg aaagattcac
    32461 gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg aagccttgtt
    32521 cggtgagata acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc accagaatct
    32581 tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta agcgtgtatt
    32641 cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc ctaaattgtt
    32701 acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca ttattgattc
    32761 tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa gggaatcaac
    32821 gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca agagcttaat
    32881 tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg
    32941 ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgtctgcg tgaaatcgac
    33001 cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga ttactcccct
    33061 cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg
    33121 actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga ttacatctct
    33181 atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct ggcggtggtg
    33241 aatacccctg ccggggaggc gagctggtat gttaatgcta tcgagtacgt gaaaccgtaa
    33301 ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc gtataactat
    33361 cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc gttctgccgc
    33421 attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt
    33481 gacgaaggga ataccgtgcc gtttatcgca cgttatcgta aggaaatcac cggcggtctg
    33541 gatgacacgc agctgcgtaa tctggaaacg cgtctgagct atctgcgcga gctggaagag
    33601 agacgtcagg cgatcctcaa atccatttcc gagcaaggca aactcaccga tgatctggcg
    33661 aaggccatca acgccaccct aagcaaaacc gaactcgaag acctctacct gccctacaaa
    33721 cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc gttggctgac
    33781 ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcacaata tgttgatgcc
    33841 gataaaggcg tggcagatac caaagccgcg ctggatggcg cgcgctatat cctgatggaa
    33901 cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg
    33961 catctggtct caaccgtggt gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat
    34021 ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc gatgttccgt
    34081 gggcgtaacg aaggcgtact ccagctttcg ctgaatgccg atccgcagtt cgatgagccg
    34141 cccaaagaga gctattgcga gcaaatcatc atggatcacc ttggcctgcg cctgaacaat
    34201 gccccggcgg atagctggcg caaaggcgta gtgagctgga cctggcgcat caaggtgctg
    34261 atgcatctgg aaaccgaact gatgggtacc gtgcgcgaac gcgcggaaga tgaagcaatc
    34321 aacgtctttg cccgtaacct gcacgatctg ctgatggcgg cccctgccgg actgcgtgca
    34381 acgatgggcc tcgatccggg tctgcgtact ggggtaaaag tggcggtggt cgatgccact
    34441 ggcaaactgg tagcgaccga taccatttac ccgcacaccg gacaagccgc aaaagcagcg
    34501 atgactgttg ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat cggcaacggt
    34561 acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg
    34621 accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcagc ttccgagctg
    34681 gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gtgcggtatc tatcgcccgc
    34741 cgtttgcagg atccgctggc ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt
    34801 cagtatcagc atgacgtcag ccagacgcaa ctggcccgca aactggacgc agtagtagaa
    34861 gactgcgtaa acgccgttgg cgtcgatctc aacactgctt ctgttccgct gttaacccgc
    34921 gtggcgggcc tgacgcgcat gatggcgcaa aacatcgtgg cctggcgcga tgagaacggt
    34981 cagttccaga accgtcagca actgctgaaa gtgagccgtc tggggccgaa agccttcgag
    35041 cagtgcgcgg gcttcttgcg cattaaccat ggtgataacc cgctggacgc gtctaccgtc
    35101 cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca ggcactgaaa
    35161 gatctgatgg gtaacagcag cgaactgcgt aacctgaaag cgtctgactt tactgatgaa
    35221 aaattcggtg tgccgacagt aactgacatc atcaaagagc tggaaaaacc gggtcgcgat
    35281 ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat gaacgacctg
    35341 caaccgggta tgatcctcga aggcgctgtc accaacgtca ccaactttgg cgcgtttgtc
    35401 gatattggcg tgcatcagga cggcctggtt cacatctctt cattgtcgaa caagtttgtg
    35461 gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt gctggaagtg
    35521 gatcttcagc gtaaacgtat cgccctgacc atgcgtctgg acgagcagcc tggcgaaacc
    35581 aacgcccgtc gcggcggcgg taatgaacgc ccgcaaaaca accgcccggc agccaaacca
    35641 cgcggtcgtg aagcgcagcc tgccggtaat agcgcgatga tggatgcgct ggcggcggca
    35701 atgagcaaaa aacgttaaac gccctgattg acccctaaac ctgcgcgata aggtgtccac
    35761 gccgcatctg gcaacgaacg ccttgtctga cataaaagtg ccggagaata tctccggcat
    35821 ttttattcca cagccaaact cacaatatat tccggcaaca tttatcactt cattaacaac
    35881 tgaaacctta attaaacatt agccagtcct ggtaattcgc tattcgaatt atattttcgc
    35941 tgcgatataa ccttgagcca catcaacatt gagtcagatt attattcaaa ccaacattcg
    36001 cacacatttt aagtattgct gatagaaacc attctcatta tcattgtgct gttgattatt
    36061 taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg
    36121 tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca gatactgcgt
    36181 ggaaaatcac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc
    36241 ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc ggcgacccca
    36301 ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg gccttattag
    36361 aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt aaccataggc
    36421 ttaattggta atccaaattc tggcaagaca accttattta accagctcac tggcgcacgt
    36481 cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca attctccacc
    36541 accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac caccatctca
    36601 tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac
    36661 ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct gacgctacaa
    36721 ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat tgccgagaag
    36781 caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc agtgatcccg
    36841 ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga tcgctataaa
    36901 gctaacgaga atgtggaact ggtgcattac gcacagccgc tactcaacga agcagattca
    36961 ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa
    37021 atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca gcatctggat
    37081 gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat tgccgatgcg
    37141 cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac ggcagaaccc
    37201 agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg tctgccgatt
    37261 ttcctctttg tgatgtacct gatgttcctg ctggctatca acatcggcgg ggcgttacag
    37321 ccgctgtttg acgtcggctc cgtggcgcta tttgtgcatg gtattcaatg gattggctac
    37381 acgctccact tcccggactg gctgactatc ttcctcgccc agggcctggg tggcggtatt
    37441 aacaccgtgc tgccactggt gccgcagatt ggcatgatgt acctgttcct ctccttcctt
    37501 gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgcctgat gcaggcgctg
    37561 ggcttgcctg ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg
    37621 gtaatgggcg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat catgatggca
    37681 ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc tgccttcttc
    37741 gggcagaacg gtgccctggc ggttttctcg ctgtatatgc tgggtatcgt gatggcggtg
    37801 ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg
    37861 gagctgccgg tctaccatgt accacacgtt aaaagcctga ttatccagac ctggcagcgt
    37921 ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat tttcctgagc
    37981 gccttcaaca gcttctcgct gagcggaaaa atcgtcgata acatcaacga ctcggcgctg
    38041 gcttccgtca gccgggtgat caccccagtc ttcaagccga ttggcgtgca tgaagataac
    38101 tggcaggcaa cggttggcct gtttacaggt gcaatggcga aagaagtggt ggtgggcacg
    38161 ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc ggcagaattt
    38221 aacctcggtg aagagctgtt cagcgcggta gatgaaacct ggcagagcct gaaagacacc
    38281 ttcagcctta gcgtactgat gaatcccatt gaagcgagca aaggcgacgg cgaaatgggt
    38341 accggggcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc atacagctac
    38401 ctgattttcg tcctgctgta tgtaccatgt atctcggtga tgggagccat cgcccgtgaa
    38461 tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatatcgc ttactcactg
    38521 gcaacattgt tctatcaggt cgccagctac agtcagcacc caacttacag cctggtgtgc
    38581 attctggcgg taatcctgtt taacatcgta gttatcggtc tgctgcgccg cgcgcgtagc
    38641 cgggtggata tcgaactgct ggcaacccgc aagtcggtaa gcagttgctg cgcagccagc
    38701 accaccggtg attgccatta atggcttcac ttattcaggt acgtgatttg ctggcgttac
    38761 ggggccgtat ggaagcgacc cagataagcc agacattgaa cactccgcag ccaatgatta
    38821 acgccatgct gcaacagctg gaaagcatgg gcaaagccgt gcggattcag gaagaacctg
    38881 acggctgcct ctctggcagt tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt
    38941 ggtgggcgct gcgttaacct tactccatcg cctgttttgg aaagcccggt atgcgtctgc
    39001 atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa ctcttgcagc acggcgtaag
    39061 ttcctttgaa agcatctcgc agggatgaaa actcgctaat gcacagatgt ggagtggcgc
    39121 gtagagtcgc ggcattcaaa caacaggtga aggaacgcca tgagtaaaaa gcagagttcc
    39181 accccacatg atgcgctgtt caaactcttt ttacgccaac cggacacggc tcgcgatttt
    39241 cttgcgttcc atttaccggc ccccattcac gcgctctgtg atatgaaaac cctcaagctg
    39301 gagtcgagca gctttattga tgacgatctg cgtgaaagct attccgatgt gctgtggtcg
    39361 gtgaaaacgg aacaaggacc aggatacatc tattgtctga ttgaacatca aagcacctca
    39421 aacaaactga tcgcatttcg catgatgcgt tacgctattg ccgcaatgca aaatcacctt
    39481 gatgctggat acaaaacgtt gccgatggtg gtgctattgt tgttttacca cggtattgaa
    39541 agcccctatc cctattcgct gtgttggctg gattgtttcg ccgatcccaa actggcaagg
    39601 cagctttatg cctccgcatt tccgctgatt gatgtcaccg tcatgcctga tgatgaaatc
    39661 atgcagcacc gacgtatggc gctgctggag ttaattcaaa aacatattcg tcaacgcgat
    39721 ctgatggggc tggtagagca aatggcctgc ttattaagta gtggatacgc taatgacaga
    39781 caaatcaaag ggctgtttaa ttacatactg caaaccggcg acgcggtacg ttttaacgat
    39841 tttatcgacg gcgttgccga acgttcaccg aaacacaagg agagtttaat gacaattgcg
    39901 gaaagattgc ggcaggaggg ggaacaatcc aaagccctgc atatagccaa aataatgctt
    39961 gaatccggag tccctcttac agacatcatg cgctttaccg gggtgtcaga agaagagttg
    40021 gctgcggcga gccagtaaat ttctgtctcg ccatttcaaa agttgcctac accctctgct
    40081 tcaacgccac cagcaggtga caaaactcga ccggatgcga aataaacggc gcatgggccg
    40141 ctttggcgaa gatatatgat tcgctgtgag gccaaagttt atccagcatc ggcaccactt
    40201 tgcgcggcac cagaccgtcg agatagccat acaatcgcaa aaacggcatg gacacgtttt
    40261 gcagcggctg acggagatcg accgttttca ggatttccag cccgccatta agcacgtcaa
    40321 cctccggcat cggtaacgcc agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg
    40381 tttcagtccc catggtttgt aacgccagga accgctccac tgtacgctga aaatcatcac
    40441 tgagttgctg ctgaaatccc gccagcacgt ccggttttat ccccggccac tcgtcacgag
    40501 cactaaaaca aggtgacgac gccacggtga ccagcgcctg aacacgctcg ggatgggtta
    40561 acgcaatctg gcttgccacc agcccgccca gactccagcc taaccaaatg gctttatcag
    40621 gtgcctgttg cagcacggct tcggccatat cagcaagtga cagcgcacca aatccccggc
    40681 tacgcccgaa gccgggcagg tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt
    40741 caatgcaacg ccacacttcg gcattcagtc cccatccgtg cagcagcaca agatgaacat
    40801 tcccctgacc tttggtctgc caccagatgt tattcatccg ctattgttct cttttgactt
    40861 acaaggatga acatatgcta acagtaccgg gattatgctg gctatgccga atgccactgg
    40921 cgttaggtca ttgggggatt tgttcggtct gttcacgcgc cacccgcaca gataaaacgt
    40981 tatgcccaca atgtggatta cccgccacac actcccatct tccctgcggt cgctgcctgc
    41041 aaaaaccgcc gccctggcaa agactggtca cagttgccga ctatgcgccg ccgttaagtc
    41101 tgctcatcca ccagcttaaa ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc
    41161 tgttactatt ggaagtcttg cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca
    41221 tcatcagcgt tccgttatgg cagcggcgtc actggcgtcg gggatttaat cagagcgatt
    41281 tgctgtgtca gccgttatca cgctggttgc actgccaatg ggatagcgaa gccgtcacac
    41341 gtacacgggc cactgcgacc cagcattttc tcagtgcccg gctgcgcaag cgcaacctga
    41401 aaaatgcctt tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg
    41461 tcgttaccac cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg
    41521 cgactgtcca ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta
    41581 taaccaacta aaatagtcaa ctattaggcc attactatga tccgtatttc cgatgctgca
    41641 caagcgcact ttgccaaact gctggcaaat caggaagaag ggacacaaat ccgcgtattt
    41701 gtgattaacc ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc gccggatgcc
    41761 gtggaagcca ccgacactgc cctgaaattt gacctgctga ccgcgtatgt tgatgagtta
    41821 agcgcaccat acctggaaga tgcagagatc gactttgtta ccgaccagtt gggttctcag
    41881 ttaacgctga aagccccgaa cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg
    41941 gagcgcgtgg agtatatgct gcagtcgcag atcaacccac agcttgctgg tcacggtggt
    42001 cgcgtttcgc tgatggaaat caccgaagac ggttacgcca ttctgcaatt tggcggcggc
    42061 tgtaacggtt gttccatggt cgatgtgacg ctgaaagaag ggatcgagaa gcagctgctg
    42121 aacgaattcc cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac
    42181 tcctactact aagttatccc ctcttttgta gattgcccga tgcgacgcta aagcgtcatt
    42241 tcgggcctac gttgaacatg tgccagacgt tggcgcagca ttgctttggc aaatccctcc
    42301 cttcgtttct ccgcgataat atgaccaacc tctcataatt taaatttacc ccgctctggt
    42361 gattctcaaa cgccagatgt tacccgtatc attcacatgg gtaccaaaca tactcctgac
    42421 atctgactac aataattagt tttagtgggt atcagtcgtg gtgccgcaat atctctgttc
    42481 ccgattggga taattagagt ttgtcgtcag aaaattgacg ttacccataa caaatgaaag
    42541 gccaggtaaa tcatgccatt agtcattgtt gctatcggtg taatcttgtt gttgctcctg
    42601 atgatccgct tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga
    42661 ttaatgcaag gaatgccgct ggataaagtt attggctcca tcaaagccgg tgtcggcggg
    42721 acgctcggta gccttgccct gatcatgggt tttggcgcaa tgctgggcaa aatgctggca
    42781 gactgcggtg gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaagcac
    42841 atccagtggg cggtggtgtt aaccggtttt accgttggtt ttgccctgtt ctatgaagtg
    42901 ggcttcgtgc tgatgctgcc tctggtgttt accatcgcgg cttccgcgaa tattccactg
    42961 ctgtatgttg gtgtaccaat ggcggctgca ctgtctgtga cccacggctt cctgccaccg
    43021 catccgggcc cgactgcgat tgccaccatt ttcaatgccg atatgggtaa aaccctgctg
    43081 tacggtacta ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg
    43141 ttgaaaggta tcgataagcc aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa
    43201 gaagagatgc cgagctttgg cgtcagcgtc tggacttctc tggtgccggt tgtgctgatg
    43261 gcgatgcgtg cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg
    43321 gagttcctcg gtgacccggt aatggcaacg ctgattgccg tgctgattgc gatgttcacc
    43381 tttggtctga accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc
    43441 aaaatcattg cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg
    43501 gtagacagcg gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg
    43561 ctgctgatgg cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt
    43621 gcggcaatca ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagccca
    43681 gagctgatgg ttattgcggt gggttccggt agtgtgattt tctctcatgt gaacgatccg
    43741 ggcttctggc tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg
    43801 tcgatgctgg aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg
    43861 gtgatttgat aacactgact gccggatgca tcgcatccgg catcaaccgc actctacttc
    43921 ttcttcgctg cagctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacgccatca
    43981 tcggcaaaca tcgtctcaag cgttgcggaa agcttgcgtc gccagttttt atactggtaa
    44041 ctggtgccag gaatattcac cggttcggcc atatccagcc agtcttccgg ctgtagtcct
    44101 aacagagcac tgttactgtc ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc
    44161 atcgacatca acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcagt
    44221 gcatccagca gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact
    44281 tcatccggat acagccccag ggttttgccc agcgttagat ccccgctctc ccagtaaccg
    44341 cgtagcgtag gcaggtcatg tgtcgccgca accgccatcg actgctccgg atacgctttc
    44401 ggtgcacgga acgtcttctc gtggtcgttt tcgaaataga gcactttgta ggagtacaca
    44461 ccgctgctgc gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc
    44521 accatacagc gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga
    44581 tagtgaacat acgcgccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc
    44641 atcgacatca catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc
    44701 tcgataaacg gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggtaatccc
    44761 cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa
    44821 tatagttcac ggtcacacca ggtttccgcc ccaccttccg ctacgccaac cgccagatca
    44881 cgatacaagc caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg
    44941 ctgtaagcca gccactgcaa ccagagataa aaatcgacgt cattacgatg ttcttcgcag
    45001 aactgacgca cttctggtga atccacgttc tgatacatct ctggccatgc aggccagccc
    45061 cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc tgcctgccag
    45121 aacaggctgt cgccctgctc tgcaacaaac tggcgaaacg cggtcatctg ctcatcatca
    45181 cgttgcgcga aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg
    45241 gagtaatcga cccagtcggc atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac
    45301 tgccaccagg cctgagcctc ttcgctaaga tggaaatctt caacggcgtt aacgtcgata
    45361 taaatcacat tcagccaacg gcgagaagac gggctgtatg ggctggcact ctccggattt
    45421 gccggataga gcgcatgaat cgggttcagg ccgatgaacg acccgccacg ttttgccaca
    45481 tccaccagca tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc
    45541 agcgtataaa gctgaacgca ggcaccccac agcttttgtt tattcagcaa cgcctgcggt
    45601 tcgtaacagc gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg
    45661 agtgtcagcg tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc
    45721 gttacatggc ctttgtactg tgttccttct tcggtggtca gcagccagct atattcgccg
    45781 ctgccctcca ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact
    45841 ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg
    45901 gtttcggcgc taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc
    45961 gccgccagcg cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc
    46021 tgccagatac gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg
    46081 gcggtattga ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc
    46141 tgcttttgtg cctcaacata ggctgcgaaa tccgccatca ccagatacgg atcgccgcct
    46201 tgtttgccga tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta
    46261 ccgctttcca gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc
    46321 gggtcgtagc ctttggccag aatagccttc acttgttcca cggtatgacc aaaaataaag
    46381 atattttctt caccgacttt ctcggcgatt tcaacgttcg caccatccag cgtaccgaca
    46441 gtaagcgcac cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca
    46501 gtcgaaattt gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa
    46561 taatccggca ggaacaccac cttcaactta tcgccaacca gtggatcgtt gttgatcacg
    46621 tcagccactt tgttgatcgc aaagataata ttcttcgcca ggtagtagcc cggtgccgct
    46681 ttcgcgccga agaggaagac gcgcggtacg cgatcagcct gcgggttttc acgaatttct
    46741 ttgtacaacg ccagaatatg cagcagattc aggtgctggc gtttgtactc atgcagacgt
    46801 ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca
    46861 aactccgcca gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc
    46921 gcatcatcag cgaatttttc cagattgatc agctgatcga gatcgttagc ccactctttt
    46981 tgcagtgatt tatccaacag agccgccagt gcagggttgc actgtttgat ccagcgacgt
    47041 ggggtaatac cgttggtgac gttatggaat ttgttcggcc atagctggtg atattccggg
    47101 aacagatctt tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg
    47161 ccaaccacac acaggttcgc catatgcact tgtttatcgt gcaccaccgc cagtttggcc
    47221 cacacttttt catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt
    47281 tcgttaataa tctgcatatg gcgcggcagt aagcctttca ccagtttcac atcccagcgt
    47341 tccagcgctt ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtaatggcc
    47401 caggcgtcat cccagctcat ctggtgctca tcgatcagca cgcgcagcag ttctggaatc
    47461 gcgatagttg ggtgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt
    47521 ttacgccccg ccagatgatg gcgacgcaaa atatccgcta ccgaacaggc acactggaag
    47581 tactgctgca tcaagcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact
    47641 ttggtcagtt tttccgcatt gatgccctgc tgttcggcac gcaggaaatc accgtcgtta
    47701 aatttagtca gatcaaacgg atgcgcgtgc gtcgcctgcc acagacgcag cggctgcgcc
    47761 acgccattac gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc
    47821 ggctcccagc gtccgtcttt cgtcacttta ccgccaatcc ctacctgcac atccagtgct
    47881 tcgttgtggc ggaaccacgg gtagttactg cggtgccagt catccggcgc ttcaacctgt
    47941 ttgccatcga caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc
    48001 gcagactgac cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca
    48061 ccgttaccca gcgccgggtc gatctcttct tccagcaggt ccgtcagatt gatgtcataa
    48121 gccttcaacg aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc
    48181 aggcgaccaa tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccaccggc
    48241 ttggcgaatg gctgagcacg cagcatttcg gccagtgctt cactcactgc tagccaccac
    48301 tggcgaggag tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc
    48361 gcttcctgaa attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta
    48421 gattttcaac acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg
    48481 cgggggatta ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcaaccactt
    48541 aaagctagtg caaaccacag gattagcagc aaatcaatgc aatacagcgc agaaaatctg
    48601 tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa
    48661 ttaagtaaag gagtgaaaca gtttcataag taaaatatcc agtgtgctcc atctcattct
    48721 taatagattt attaagatca tctttttaga tggcactttc atcaggaatg aagaagaaac
    48781 ccttgcttaa atgaatctga tgaacataag ggaaaccagt attcacgctg gatcagcgtc
    48841 gttttaggtg agttgttaat aaagatttgg aattgtgaca cagtgcaaat tcagacacat
    48901 aaaaaaacgt catcgcttgc attagaaagg tttctggccg accttataac cattaattac
    48961 gaagcgcaaa aaaaataata tttcctcatt ttccacagtg aagtgattaa ctatgctgat
    49021 tccgtcaaaa ctaagtcgtc cggttcgact cgaccatacc gtggttcgtg agcgcctgct
    49081 ggctaaactt tccggcgcga acaacttccg gctggcgctg atcacgagtc ctgcgggcta
    49141 cggaaagacc accctcattt cccagtgggc ggcaggcaaa aacgatatcg gctggtactc
    49201 gctggatgaa ggtgataacc agcaagagcg tttcgccagc tatctcattg ccgccgtgca
    49261 gcaggcaacc aacggtcact gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc
    49321 cagcctgacg tcactcttcg cccagctttt cattgagctg gcggaatggc atagcccact
    49381 ttatctggtc atcgatgact atcatctgat cactaatcca gtgatccacg agtcaatgcg
    49441 cttctttatt cgccatcaac cagaaaatct caccctggtg gtgttgtcac gcaaccttcc
    49501 gcaactgggc attgccaatc tgcgtgttcg tgatcaactg ctggaaattg gcagtcagca
    49561 actggcattt acccatcagg aagcgaagca gttttttgat tgccgtctgt catcgccgat
    49621 tgaagccgca gaaagcagtc ggatttgcga tgacgtttcc ggttgggcga cggcactaca
    49681 gctaatcgcc ctctccgccc ggcagaatac ccactcagcc cataagtcgg cacgccgcct
    49741 ggcgggaatc aatgccagcc atctttcgga ttatctggtc gatgaggttt tggataacgt
    49801 cgatctcgca acgcgccatt ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc
    49861 actcatcacc cgtgtgaccg gcgaagaaaa cgggcaaatg cgcctcgaag agattgagcg
    49921 tcaggggctg tttttacagc ggatggatga taccggcgag tggttctgct atcacccgct
    49981 gtttggcaac ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat
    50041 ccaccgtgcc gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca
    50101 tgcgctggcg gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag
    50161 tctgttcaac catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga
    50221 cagcttgctg gaaaatccgc agttggtgtt attgcaggcg tggctgatgc aaagccaaca
    50281 tcgctacggc gaagttaaca ccctgctagc ccgtgctgaa catgaaatca aggacatcag
    50341 agaagacacc atgcacgcag aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg
    50401 taatccggat gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccgggctg
    50461 gttctatagc cgcattgtgg caacctcggt gctgggtgaa gtgctgcact gcaaaggcga
    50521 attgacccgc tcactggcgc taatgcagca aaccgaacag atggcacgcc agcacgatgt
    50581 ctggcactac gctttgtgga gtttaatcca gcaaagtgaa attctgtttg cccaagggtt
    50641 cctgcaaacc gcgtgggaaa cgcaggaaaa agcattccag ctgatcaacg agcagcatct
    50701 ggaacagctg ccaatgcatg agtttctggt gcgcattcgt gcgcagctgt tatgggcctg
    50761 ggcgcggctg gatgaagccg aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta
    50821 tcagccacag caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg
    50881 tgatttagat aacgcccgta gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa
    50941 atatcacagc gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac
    51001 cggcgataaa gccgccgctg ccaactggtt gcgtcatacg gctaaaccag agtttgcgaa
    51061 caaccacttc ctgcaaggtc aatggcgcaa cattgcccgt gcacaaatct tgctgggcga
    51121 gtttgaaccg gcagaaattg ttctcgaaga actcaatgaa aatgcccgga gtctgcggtt
    51181 gatgagcgat ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg
    51241 taaaagtgac gcccagcgcg tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt
    51301 tatcagtcat tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agctgattca
    51361 actcaatacg ctgccggaac tggaacagca tcgcgcacag cgtattctgc gagaaatcaa
    51421 tcaacatcat cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa
    51481 tcatccggaa gtgccagaac tgatccgcac cagcccattg acccaacgtg aatggcaggt
    51541 cctggggctg atttactccg gttacagcaa tgaacaaatt gcgggcgaac tggaagtggc
    51601 ggcaaccacc atcaagacgc atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg
    51661 tcaggctgcc gtgcagcacg cgcagaaact gctgaagatg atgggttatg gggtgtgagt
    51721 ttagccggat aatgcattgg atccggttta catctttaca tcattcaata ctcacccgcg
    51781 ttacgccatc tgtttctatc aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga
    51841 tattggtcag cagatggcat gaaggatggg cgaccttaaa ttctcccgcg cccgccagcg
    51901 ccatcggcag taccagttgg tcggcgagat attcccccac cgccgctggg cttgccaggt
    51961 agcgtttcac ctctttcact aactgcgcgg ctaccacctc ggcactgacg cgcttttcac
    52021 cgacgacgaa aaagcgttca gtgatatttt cactttcgac ttccagcgag acggtattac
    52081 ccggcccctg gtcgcgcggc aggttatgaa tattctgttc atgcagggaa aaactccccg
    52141 ccagtgtagc gatttcacgc tcagcaacat ggcgcggcac accagctaat agaacttctc
    52201 cacgcatctg cacaatgttc ccgcgctcgc caagttgcaa ggtgttaaac gatgccacgg
    52261 gcgagacttc cgttgccacc acgccgcctc cggcaggata aaaaccgtga cgtaacagcg
    52321 tggtttgctg atgaattcct attttcgcca gcagtggctc cagcacccgg cggataaaat
    52381 ccgcaggcgg ggccgacggg ttatcggtgc ctccgctcac ttcaacacgc gaaggtccat
    52441 cggcaaacca cagcgcgggc agcaccgttt gcagcaccag cgtacagctt ccggcactac
    52501 cgatagcaaa gcggtactcg ccgccgcgca cggtgccagg ccggaagacc agacgctgcg
    52561 accccagctc cgcgccttcc accgttgccc tacaaatttc cgcagccgct tttaccgcgg
    52621 tcagatgctg gcgcaacagc cccggtttcg cccgcccggc acgaatgctg gtgatggtaa
    52681 atggctggcc ggttatcatc gacaggctca gcgccgagcg caggatctgc ccgccacctt
    52741 cgccctgtgc gccatccagc gcaatcatcc ttttcatcat ttatcctttt acgcacacca
    52801 cctgacgcag ggtatagata acttccacca gatcgctttg tgccgccatc accgcatcaa
    52861 tatctttata cgccatcggg atttcgtcga tcacttcggc atctttacgg cattccacat
    52921 gcgcggtggc acgaatttga tcttccacgc tgaacagttt tttcgcttta gtacggctca
    52981 ttacccgccc ggcaccgtgg ctgcacgaac agaacgactc ttcatttccc agcccacgga
    53041 cgataaagct ttttgctccc atcgaaccgg gaataattcc atattgacca gcacgcgcag
    53101 acaccgcgcc tttacgcgtc acgtagatct cttcaccaaa gtgctgttct ttttgcacat
    53161 agttgtggtg acagttgatc tcttccatcg ccagcgtttg tggctgtctg accgttttct
    53221 gcgtaatgct ctgcaacgcc gttaccacgt tttccatcat cgcatcgcgg ttaaggctgg
    53281 cgaaaagctg cgcccaggcc acggctctta ggtaatcatc aaagtattcc gtaccttcca
    53341 taaagtacgc caggtcgcgc gatggcaacg tttcaagctg gtcctgcatc tctttttgtg
    53401 ccaaatcgat aaagtaagtc ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca
    53461 taatccacac ctggtctgac tcatcaaggc agatttcaat aaagtggtta cccgttccca
    53521 gcgttcccag gtgtttatag ttgttagtgt taaggaaacg cggatatttt tgcgttaacc
    53581 actgataacc ggcttcaagc tcagcccatt tcgcatcgac gttaacaggc gggttttccc
    53641 atgcgccttt atcacgttta caacggccag tggtacgccc gtgcggcacg gccatttcaa
    53701 tcgcctgacg cagctctgcc aggttttcag gcaggtcttc cgccgttaac gcggtacgca
    53761 gcgcgttcat tccacagcca atatccacgc ccaccgccgc cggaataatc gcccctttgg
    53821 tcgggatcac gctaccaatg gtggaacctt tacccaggtg tacatcaggc attaccgcaa
    53881 tatgtttgaa aataaacggc atcttcgccg tattaataag ttgctgacgc gcatcggctt
    53941 ctaccggcac gcctttggtc cacattttta ccggggcatt ttcagtggtc agtaattcgt
    54001 aattcatttt gtttttctct tttcgttgtt tgctgtcgtg ataattgcaa ccgtcgtgcc
    54061 agaaaattaa ataagcagat taatttttta attcattgtt tttaaatagg ttaaatcttt
    54121 acgtccgtaa acggagattt tccacaaaac caattcaccg ataatgaaat atcgtctttt
    54181 ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggtac cgtgctggat
    54241 tatgccggac gcggcagtca gcgctggtcg aaatggcgtc cgacgctttg tttatgccag
    54301 caagaatcgt tagtcatcga tcggctggaa ttgttgcacg acacccgctc gcgctcgcta
    54361 ttcgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaacagaagt ggtgagcgtt
    54421 gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct gcatgatttc
    54481 gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat caccaccggc
    54541 acccacgtcg cgcagatttg ctggtttctg ctggcggaag cgcgttacct gcccgcccgg
    54601 ctgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcgcagg tgaagtgacg
    54661 attatcgatc tcgatttaag ccgttataac gccatcgcca gccgctttgc cgaggaacgc
    54721 cagcaaacgc ttgattttct taagtccggc attgccacgc gtaaccccca cttcaaccgc
    54781 atgattgagc agatcgaaaa agtggcgatc aaatcccgcg cgccgattct gcttaacggt
    54841 ccaaccggcg cgggcaagtc atttctggcg cgacgcatct tcgagttaaa acaggcgcgg
    54901 catcagttta gcggcgcgtt tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcc
    54961 atgtcgacgc tgtttggtca tgtaaaaggc gcgtttaccg gggcgcggga atctcgtgaa
    55021 ggtttattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg cgaactgggc
    55081 gcagacgaac aagcaatgct gctaaaagcc attgaagaga aaacctttta cccgtttggc
    55141 agcgatcgcc aggtgagcag tgattttcag cttatcgccg gaacggtgcg cgatttgcgc
    55201 cagctggttg ccgaaggcaa atttcgcgaa gatctgtacg cgcggatcaa tctctggacc
    55261 ttcaccctgc cgggtctacg ccagcgccag gaagatattg aaccgaacct tgattatgaa
    55321 gtggagcgcc acgccacact cactggcgac agcgtgcgtt ttaacaccga agcgcggcgc
    55381 gcctggttgg cttttgcgac ctctccccag gcaacatggc gcggtaactt tcgcgaactt
    55441 tctgccagcg tcacgcggat ggccaccttt gccactagcg gacgcatcac tctggacgtg
    55501 gttgaagatg agataaaccg tctgcgctat aactggcagg agagccgccc ctccgcgctt
    55561 acggcgttgc tgggcacaga ggcagaaaac atcgaccttt ttgacaggct gcaactggaa
    55621 cacgtgatag ctctctgtcg ccaggcaaag tcgctttccg ctgccggacg cctgcttttt
    55681 gatgtttcgc gccagggcaa agccagcgtc aatgacgcgg atcggctacg caaatacctg
    55741 gcgcgttttg gtctgacgtg ggaagccgtg caggatcagc acagctccag ttgaatatga
    55801 tggtccgtca gcacctgcat tacgcttact ggcggcgggg cgtcggtgta gacggcatct
    55861 accatgctga tgctgcccat attgaccatc gcgttacggc caaatttcga gtgatcgaca
    55921 accagcataa cgtggcgcga gttctcaata atggcgcgct tggtgcgaac ttcgtggtaa
    55981 tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat gccgaaatca
    56041 aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg
    56101 cgtaattcgc caccggcgag aataatgcgg aaatcttctt ttaccatcaa cgtgttagca
    56161 acattgagat tgttggtgac aatacgcaaa ttgctgtgat taagcagcgc gtgcgccacc
    56221 gcttccggcg tggtgccgat atcgataaac agtgtcgagc cgttggggat ttgctccgcc
    56281 actttgcggg caatcctctc tttttcttcg gtctgggtgg ctttgcgatc gtgccacggc
    56341 gtgttaaccg aactggaagg cagcgccgca ccgccatggt gacgcaggat caggttttgc
    56401 tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa atgctctacc
    56461 agctcttcag tactgacata accctgctgt ttaaccagtt cgataatacc gttgtgacgt
    56521 tgtgtttgtt tcatttataa atccctggaa ttattttcgt tttcgcgcat tgagcgaatc
    56581 aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt tcgccatcga
    56641 catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga taattaaccc
    56701 acgttgcagg taaatgccac tttgcggatc gcgttcgcca cgtagccaga cgtagcccat
    56761 cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac
    56821 atagccgctt aacagggcgc taatgagagt aataacaatt agcttaccgc taccgaggcg
    56881 tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga tatgcatcag
    56941 cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca gtgttggatc
    57001 gaatggccag gccagccata acatcacttc ctgatcgccg agaatttgca tggcgataaa
    57061 caccaccacg caggcgatca tcaccaccca ggttaccgga cctgcgcgtt cacgcaaggc
    57121 agcaaagaaa ggataacggc gataatgcag accactgccg gtatggcctg cctgccagct
    57181 ggccgccaga taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgtac
    57241 gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt gttgaatcgt
    57301 gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc
    57361 aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg ggacagtata
    57421 aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gctgccaggc ttcaaaaccg
    57481 ccgtcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc cgcgcctttg
    57541 ctgctattgc cgtgataaca catcaccatc accggagtgt caaagtcgtt atcacgcata
    57601 aacgcgccca gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc cattgcgaaa
    57661 ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg
    57721 acggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac aaacaagtgg
    57781 gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg ttatacatat
    57841 cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat atgttcgata
    57901 acgaacattt atgagcttta acgaaagtga atgagggcag catggaaacc aaagatctga
    57961 ttgtgatagg gggcggcatc aatggtgctg gtatcgcggc agacgccgct ggacgcggtt
    58021 tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa
    58081 aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg gtcagcgagg
    58141 cgctggctga acgtgaagtg ctgctgaaaa tggctccgca tatcgccttc ccgatgcgtt
    58201 ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcgcatt ggtctgttta
    58261 tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aactggtttg cgttttggcg
    58321 caaattcagt gttaaaaccg gaaattaagc gcggattcga atattctgac tgttgggtag
    58381 acgacgcccg tctggtactc gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc
    58441 ttactcggac tcgcgccacc tctgctcgcc gcgaaaacgg cctgtggatt gtggaagcgg
    58501 aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt aacgccaccg
    58561 gcccgtgggt gaaacagttc ttcgacgagg ggatgcatct gccttcgcct tatggcattc
    58621 gcctgatcaa aggcagccat attgtggtgc cgcgtgtgca tacccagaag caggcctaca
    58681 ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac gaattttcca
    58741 tcatcggcac caccgacgta gagtataaag gcgatccgaa agcggtgaag attgaagaga
    58801 gtgaaatcaa ctatctgctg aaagtgtata acgcgcactt taaaaaacag cttagccgtg
    58861 acgatatcgt ctggacctac tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc
    58921 cgcaggctat tacccgtgat tacacccttg atatccatga tgaaaacggc aaagcaccgc
    58981 tgctgtcggt attcggcggt aagctgacca cctaccgaaa actggcagaa catgcgctgg
    59041 aaaaactgac gccgtattat cagggtattg gcccggcgtg gacgaaagag agcgtgctgc
    59101 cgggtggtgc cattgaaggc gaccgcgacg attacgcagc tcgtctgcgc cgtcgctatc
    59161 cattcctgac tgaatcgctg gcgcgtcatt acgctcgcac ttacggcagc aacagcgagc
    59221 tgctgctcgg caatgcggga acggtaagcg atctcgggga agatttcggt catgagttct
    59281 acgaagcgga gctgaaatac ctggtggatc acgaatgggt ccgccgcgcc gacgacgccc
    59341 tgtggcgtcg cacaaaacaa ggcatgtggc taaatgcgga tcaacaatct cgtgtgagtc
    59401 agtggctggt ggagtatacg cagcagaagt tgtcgctggc gtcgtaagga tgatgcccgg
    59461 ttgtgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
    59521 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
    59581 tctggtttct attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg
    59641 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
    59701 gaagccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
    59761 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
    59821 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
    59881 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtagttcct
    59941 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
    60001 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
    60061 caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg
    60121 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcgtctac
    60181 ccagacctga tgctgtgtgc cctgttctgg ctgctttggt gggttctcaa tcgcctgccg
    60241 cgcgcgttct ttcaagatgg attgattgtt gaaggcccag gcattgattt gggtggtaat
    60301 gacgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
    60361 acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
    60421 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
    60481 atcgccggat acccaatcag tcatatactg gcaggcaaat cgttgcaaaa ttttccattc
    60541 atcctcacgc caattttctt tactggcaag gtttatacgc cgtagagaaa atacttcgtg
    60601 aatattgtca aactgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
    60661 ctcatcgcta ttttgcggat caggccctgg gctactattc catgcattga ttaaggaata
    60721 cgagatcgct cgtaacgatg tactccgtaa taccttttgt tcctgctgac tcaggcaata
    60781 ttcacaacaa accacaaatt tctgcggcac cggataacga cgaaaaatat gataaatctg
    60841 ctcaattaat gcctgcatgg cggggctggg ttgcataatt accccctcgc ccgctgatcg
    60901 cgtgtaaacg gttgataaac aaaacgagca ttcgccacgt tcaggcgaat aatggcacta
    60961 cgagagaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcttcagcg
    61021 caattttcca ctgtgtaata acgagcgtag agtttcagtt ccgcttgctt atcacgaaat
    61081 ccgcgtaaac tgttatcgtt atgaaatttc caggcatctt taaagacctg aaaaatgtag
    61141 atatcctcaa tccaggactc acatttttta agcgggcgct ttgctatgat cacccgctca
    61201 tccgggttta gcatcgccgt aaattgaacg gtgaggtcat ttgtcgtgtt cagatcacat
    61261 tgaacatctg gtacatcact gatatagggg tcaaatccaa agtcagcacc acaggcacaa
    61321 agacggctaa tggtggcggt atcgagggaa aatcccggcc atacattgct ggacgttaag
    61381 ccaatattta atgtgacatt atatcgaagc aatccctcaa gaccgtcagc accgacgaac
    61441 ttttcccctt catccagggc ctggttaatg agttgctgaa caatgccttc atcccagtat
    61501 gccgatttgc agttggcaat cgtaatatcc acgcctgtga atggatcagt accgacaagg
    61561 cttgcaaata atgtattaac taattccatt tttattatcc gccttaatgc aacgttttat
    61621 acatcggcac ataacctaca tcttccacca ggctctgccc ctgcggcgtt aaaaaccact
    61681 ccaccaattt ttgtgtttct gacgtcatat tttcccgcgt taccataaag gcatcgacga
    61741 tatagggata tttgccgttg cgaatgtttt cggcagtcgg tgcaataccg ttaatcgcta
    61801 gcagttttat atttttatcg gcgttcattt gtgttgcgta ataacggaag gtgtaaccta
    61861 ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc atcatacttg
    61921 ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg gattgcatca
    61981 ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga tcgttaccgc
    62041 caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt tgttcagtca
    62101 gggaattaac cgggttatct acattgacga tgaaaacaaa cgcttcacgg gcaaaaggcg
    62161 tgtaaatcaa agtaacgccc gattcctccg cgcgtttttt ctgcccaccg gaaggttgag
    62221 ccacaaagat aatatcggca ttacctttaa caatcttgtt atatgcttcg ggagtacgag
    62281 agttcgccag atattcccat tcgtgaaaat cttccggcaa aacgcttagt gcataaaagg
    62341 cagaggcata aatggggtac gccgccgttg cgccatcaag tcgcggccag ttttgcgtga
    62401 actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta tcgggtcgcc
    62461 aggcccaggt atcaatattt tcacgcacac cgacgccggg attaagctgt ttatataaat
    62521 aagcctggta accggcgaca aaaccaagca tgagaaggat aaacaagaga gaattacggc
    62581 aacgtaaccc ggacgtgccc gttaaaggat atctggtcgg ccagccgtat cccagcgaaa
    62641 aactgatatg cgcgccaaca ggtataatga ctaaccacca gtactcacca ccgactaatg
    62701 aggctaacaa aacgcccagc caggggagca taagatcagc caaccaaccc gctcccatct
    62761 ggcgaaattg ataatctgcc agatgcagcg cgattatcca gacccccagg gttaataatg
    62821 cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct ggtgcactat
    62881 cgtccgccca cactcgcccc gagaaaaata gcattaatgc gcccagcgcc agcataaaaa
    62941 tgttgagcag aaggtaaatt gttggcgtga taaagatatt acaaacttta ataataccgg
    63001 caagtccgac acccagcatg gcaataaccg ccgccaaaaa ctgcgccaga atggggatgc
    63061 cgaaaaaagt cattaccagc gaggtcaaaa tccattttct gttttgcatt attctttcca
    63121 ttctttctga atggcgaaat tatactcccc gagtcccctt gccccttctg gacacttttc
    63181 cgaaatgatg gcgaaaaaaa cgggaccctt tggccccgtt ctatttatta gtgaaattac
    63241 aatctcaccg gatcaatatg ccagatatga tcggcgtact ctttgatagt acggtcagaa
    63301 gagaagtagc ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc
    63361 tcgtagagtt cgtcgacttt atcctgacaa tcgacatagc tgcgataatc cgccagaacc
    63421 tgatagtgat cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct
    63481 tccggactga atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc
    63541 tcgtagtatt cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgcagtg
    63601 ttaccaaaga taaagatatt gtcagcaccg acatgatcca gcatctcgac attcgcaccg
    63661 tccaacgtac cgatagtaag cgcaccgtta agcgcaaact tcatgttact ggtgccggaa
    63721 gcttccgtcc ccgccagcga aatctgttca gacagatcgg ccgccggaat gatcaactgc
    63781 gccaggctaa cgctgtagtt cgggatgaac acgactttca gcttgtcgcc aatctgcgaa
    63841 tcgttgttga tcactttcgc tacgtcattg atcaaatgaa taatgtgctt cgccatgtaa
    63901 taggccgaag ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccggg
    63961 tcggccttga tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg
    64021 tattcgtgaa tacgtttgat ttgtacatcg aacaacgcct ttggattcac caccacattc
    64081 agctgctggg cgatatactc tgccagacgc tttttgttct ccagcttcgc ctgatgcaca
    64141 gcgtgattaa ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg
    64201 gtgcgccagt tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc
    64261 gctagccagc gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt
    64321 ttcgcaaagt cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg
    64381 ttaactttgt ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat
    64441 tcatcaatga tcgacgcccg tcccagcaga tcggtatcgt tcggatactg ttcctgcaag
    64501 gttttcagga aatagtcgtt gatttcaaag atgatctgca ggtgacgtgg cagaatttta
    64561 cccagcatat caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag
    64621 gagaagacct gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc
    64681 agcagacgca tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg
    64741 attttatccg ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc
    64801 tgaatggtcg aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag
    64861 gtggagtcat ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact
    64921 gccgcgaagt agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgggca
    64981 ctccacaaac gcaacgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa
    65041 gcgactccca gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg
    65101 cgaccaccaa aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttaccg
    65161 tattccagcc agtagtctgg cgactctttc tggctaccgt taacgatgtt ctgcttgaac
    65221 ataccgtagt catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa
    65281 tcaaggaagc aagccgccag acgtcccagg ccaccgttac cgaggcctgg gtcattttct
    65341 tcatcaatca gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg
    65401 taaattccta gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac
    65461 aggtagtaaa cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc
    65521 tccacgagac gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg
    65581 acgaccgggt cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct
    65641 tctacgctaa gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa
    65701 aactatttca agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa
    65761 tccattgcca tagcctgacg ttgcacaaac cgccacagtg aaggacggga ccacagtaca
    65821 aaagcacgtc gaatagcccg taacagcgac caggcattac tatcttcaaa gacaaaccca
    65881 ctggcgacgc catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca
    65941 ccggtgcgcc gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg
    66001 cacggttcaa aacggctggg caccagaatg acgtccgcgc cgcccataat gcgatgcgaa
    66061 aatgcttcgt gatagccaat ctgaacgccc acctggccgg ggtattccgc tgccgccgca
    66121 aggaaacctt cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc
    66181 tccagaagac ccggtaaggc ttccagcacc aggtcgagac ctttctggct ggtcagacgg
    66241 ctcaccactg caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac
    66301 tggcgcttat tttccgcttt atcttccaac gtatcgcggg tgtaacgcga ggccaacagt
    66361 aagtccgtct ctggactcca gattttctcg tccacgccgt tcagtacgcc ggaaagacgc
    66421 ccttcacggt gacgctgttg caacagacct tccataccgt aggcaaactg cggttcggtg
    66481 atctcgcgag cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc
    66541 ttcaggaaag agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc
    66601 aattggatgt cattcatgtg atgtgcataa aacatgcctt gataggccaa gttgtgcaca
    66661 gtaaacaccg acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca
    66721 tgccagtcgt gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt
    66781 tctgccccaa cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag
    66841 ttggtatcgt gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat
    66901 atgccaaccc cgttgtaatg accgaacaac agcgtgatat gtccggcgaa ggtatcacga
    66961 cgggatacta cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt
    67021 acgcgagcgt caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc
    67081 agaccgccgg ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc
    67141 gctcctgttt atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt
    67201 ctgaacgata gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg
    67261 gaataacaca agcacgatcg atgacgcagc ggcgcagacg gcacgagcga cctacccata
    67321 cttccggtaa caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga
    67381 acagaacgga ctgcaccacc accgaaccgg agatcacaca accgccggat accagtgagt
    67441 taagggtcat cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt
    67501 cattgtaggt gcgaattggc caattgcgat cgtacatatc cagttccggc accacagagg
    67561 ccagatcgag gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct
    67621 cggcatccgg gtcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg
    67681 cttcggtgat cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcacgat
    67741 cgtcttcttc cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg
    67801 ccagagattt gctcggatcg ttcggcattg acggtgggtt agcaggtttt tcaacgaatt
    67861 cgataatttt atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg
    67921 gtactggcat acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac
    67981 gcgagtagtc ttgcttgtag atatggtcgc ccgccaggat caccacgtat tccgctttat
    68041 aacggcggat aatatcgagg ttttgggtga ccgcatctgc ggtgccgcga taccagtttt
    68101 cccctttcat tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga
    68161 agaatgacca gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca
    68221 cgcccatacg acggatcccg gagttgatgc agttagacag cgcaaagtca ataatgcgga
    68281 acttaccgcc gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg
    68341 taccacgtcc tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca
    68401 ttaagtgatc gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa
    68461 cacacacaat ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc
    68521 agcgaatggg ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag
    68581 cgtggcgtta attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc
    68641 gttttgccac tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc
    68701 gtcgccttct tcccaccagc gattctccac caaagcggga atgcgcttgc gcagatggat
    68761 taacgcggcg gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa
    68821 ttggttatcc tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc
    68881 ggccagtaac atcggcgtac cctgggagag caacaacgtt gttaacaggg cgtgaatgct
    68941 gtcgcgccgc cgttcaacca ggtcaagaga accgcctaac ccttctttac catgattgtt
    69001 actgtaattg ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg
    69061 gttgaagcaa acgcagtcgc gaagcgtaaa accgtcatgc gcggtgacga gattaatcgc
    69121 ggcactcggc agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc
    69181 aaacgccccc agaggcaaat cataatgtag ccagaaacga cgggcagcat cgcggaaatg
    69241 atcgttccac tcggcaaaca gcggcgggaa atttcccacc tgataaccac caggagcgat
    69301 atcccacggt tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt
    69361 aaacaacggc gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa
    69421 gcggaaacca tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc
    69481 caccaccgcc ggatgactca aattgagcgt gttgccgcaa ccggtccagt tgtgataatc
    69541 gccgtcttct cttatccaat aatagctacg gttatcgatc ccacgcagcg agaataacgg
    69601 gccgtcgagg tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc
    69661 cgctttatgc agtgctttga ttgcatcgcg aaactcatcc agcgccgttt ctggcgagca
    69721 ggcatacgcc ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag
    69781 ccccatgcgt tgcagacgtg gttcactggc aaactgcgcc actggcagca gttccagcgc
    69841 ggtaatgccc aattgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt
    69901 gccacggatc tcgaccggga tctccgggtg caagtacgtt aatcctttga catgggcttc
    69961 ataaatgatg gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg
    70021 atcaaccacc actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt
    70081 atgaccggcg tgcagcagcg ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg
    70141 atcaatcaac aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg
    70201 aacgcgataa ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc
    70261 gccactgtgc cctggcaagt catagcgatg ttcctggcca ttggcgtcaa agacacacag
    70321 ttctacccgc tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg
    70381 cgcgccgagg ggagcgggtt tgccaatggc gagttgtgtc attctgcctc ccgaaccagc
    70441 cagatagtgg ccagcggtgg tagcgtcagg cttagtgaat gctgacgacc gtggctggca
    70501 atctcatcgc tgtgtaccgt gccgccattg cctgcattac tgccgtgata gtgcatggaa
    70561 tcggtattga ggatttcacg ccatttgccc ggctggttta tgccgaagcg ataatcatga
    70621 cgcggtaccg gcgtaaagtt actggcaacg atgatttcgt taccctcttt atcgcgacgc
    70681 acaaagatca gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg
    70741 tcaaaatcca gttcatgcat tgctttatgg tggcggtagg tgaggttcag atcgcgcacc
    70801 agacgctgga caccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg
    70861 gcgtcatggt tccactcgcg gccctgggca aattcgttac ccatgaacag tagtttcttg
    70921 cccgggaatg cccacatcca gccatagtag gcgcgcaggt tcgcgaattt ctgccatgcg
    70981 tcgcccggca tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac
    71041 ggcaggacga agttttcagt gtagttgtag agaatcccga aggtcagttt atcgtgatga
    71101 tactgacgat aaaccgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg
    71161 ttccacttgt accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa
    71221 tcggtagact cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg
    71281 gtattacgca agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc
    71341 cactccccct ctttacggct gtagtcgcga taaatcattg acgccaccgc atcgacgcgc
    71401 agcgcatcaa taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg
    71461 acttcacggc gaccatagtt gtagatcagc gtgttccagt cctgatgata gccttcacgc
    71521 ggatcgctgt gttcatacaa gttcgtgcca tcaaattcgg caagcgcaaa gtcatcagtc
    71581 gggaagtggc ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcggcatca
    71641 atgaaataac ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt
    71701 ggctgataac cccaactgcc atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg
    71761 gtaaagccca tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac
    71821 cagaaattgt tgtcggtgtg gcgacgccag gaacccaggt gaacttcata aatagagatt
    71881 ggcgcatcaa actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc
    71941 ccgcaaataa gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac
    72001 ttcagacgca agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc
    72061 gccccaggga taaacagttc ccaaatgccg ctctctttac gcagacgcat cgggtgacgg
    72121 cgaccgtccc agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccaa
    72181 acagagaaac gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaggtttca
    72241 tacgggcgca ggtgagtacc ttcagataat agccaggcat ccatttcctg gattagcggg
    72301 ccaaaacggt aaggatcatc aatcaggttt tgctgaccat gccagacaac agccaactga
    72361 tagcggaaaa aattcttacg tcgcggaatg acaccgctaa agaatccccg tgagtcgaga
    72421 cactccagtt ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg
    72481 tcgggtaaaa gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa
    72541 aaaggatccg caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg
    72601 gacatgcttg tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg
    72661 ttgtgacatt tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct
    72721 cacttcaggt aaggctgtga atactcatgt attcagccac ccttaaagaa tagccaatgc
    72781 tctatttaac tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac
    72841 aggggcggca tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc
    72901 gacgcagcgg ctccgcggcc ccccacagca gctggtcgcc cactgtaaag gctgacagga
    72961 actctggtcc catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa
    73021 cggcagctgg ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc
    73081 acggattgtg cgcagccagc agttcttcca cggtcggaat agacacatct tttttcaatt
    73141 taatagtgaa tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta
    73201 ccggaattac ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt
    73261 cgcggctctg accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccaacg
    73321 gcacgccaaa gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt
    73381 cgatatcgag aatagcagag gacggggtcg cgagttcatc tgccacatgg ccatacagat
    73441 ggcccatctg ggttaataac tcacgcatat gtcgcgcacc accgccggaa gcggcctggt
    73501 aggttgcaac ggacacccaa tcaacaagat cattggcgaa taaaccactc aacgacatca
    73561 acatcaggct tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt
    73621 cggtaatgac gtcctgattg acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca
    73681 gagacgatgc tgcgtcaatc cagtaacctt gccatccgct ttcacgaagc tttggataga
    73741 tttcgttggt ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct
    73801 ccagatcaaa ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctggc
    73861 caagctgaga agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca
    73921 tgcgttgcat gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca
    73981 taagcgtttt tttcctgcaa agatgtgtgc tgtataaatg tgccggtctc ctcttggcac
    74041 atctttcacc atacaaaaag cagccaaagt cgcaagtgaa attaatcaat gatagcgaag
    74101 ccatcagtaa tgcgacttat cctgctttgt tagcacgcag aaagtccgcg gcaattatca
    74161 gggaatttga gttatgaatg aaatcatttc tgcagcagtt ttattgatcc tgattatgga
    74221 tccgctcgga aacctaccta ttttcatgtc cgtactgaaa catactgaac cgaaaagacg
    74281 gcgggcaatc atggtgcgag agttgcttat tgctctcctg gtgatgctgg tgttcctgtt
    74341 tgcgggtgag aaaattctgg catttcttag cctacgagca gaaaccgtct ccatttctgg
    74401 cggcatcatt ctgtttctga tcgccattaa aatgattttc cccagcgctt caggaaatag
    74461 cagcgggctt ccggcaggtg aagagccatt tatcgtgccg ttggcaattc cgttagtcgc
    74521 cgggccgact attctggcca cgctgatgtt gttgtctcat cagtacccga atcagatggg
    74581 gcatctggtg attgctctgc tgctggcctg gggcggcacc tttgtcatcc tgctacagtc
    74641 ttcgctattt ttacgtctgc tgggcgagaa aggggtgaac gcacttgaac gcctgatggg
    74701 attgattctg gtgatgatgg caacccagat gttcctcgac ggcattcgaa tgtggatgaa
    74761 ggggtaacga ggagagtttt tcgaaaagaa cccggtagcg tcacaactac cgggcgaaca
    74821 aacttaactc aacagctgga acgcaatcat cccaacgatg gcaccgacag tgccgaggat
    74881 ggtttccatc atggtccagg ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc
    74941 gaacaaccag aaaccggcgt cgttaacgtg gctgacaaca atcgaaccac cagcgataca
    75001 aatcgacagc gccgccattt gcgcaccgga gtagttcagt tgttcaataa ccggcatcac
    75061 cagtcctacc gccgttaaac aggctacggt ggcagaaccc tgaatgatgc gcactgcagc
    75121 tgccagcacg aagcaggtga tagcaatcgg caggcccatg ccggttaacg cttcgcccag
    75181 tgccggacct acgccagagt caaccagcac ctgtttgaac acgccgcctg caccaatcac
    75241 cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa atctccatca ctttgtcttt
    75301 tggcatgccc tgacgcattg ccaggccata aatcgctacc agacaagcaa ccagaatcgc
    75361 ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg gtagagcctt ctggcacaaa
    75421 acgcgcggca atggttttca gccccaccag caccagcggc aacaggatca gcgacaggct
    75481 gaatccgaaa gatggcattt tgccttcgcc gagatgcggt tcgctgatgt cgtcaggaat
    75541 atgcaactcc acgtagcggc tgatgaaatt accccacagc ggcccggcaa taatcattcc
    75601 cggaattgcc gcacacaggc caatcaggat catccagcca aaatcggcgt tcatctgcga
    75661 tgcgaggagc attggcgctg gcccaggcac caggaacgca gccgcggccg ccacgcctgc
    75721 gaataatggg attaccagct tcaccaggtt cgtaccggtg tggcgcgcca ttgagaaagc
    75781 aacgctaatc agcagaacaa tcgccacttc aaagaacagc ggtagcgcac agaccagccc
    75841 cgcaaggccg atggcataat gcgcgcggct gtgaccgaag gatttgagca ttttgacggc
    75901 aatctgatcg actgcgccgg tttcatgtaa gatcttgcca aacatagctc ccagggcgac
    75961 aaccaccgcc aggaagccga gggtgcctcc catccctttt tccatcgtcg ctgcgatttt
    76021 atcgagcggc ataccggaaa aaaggccagc ccccatggac accaccatta aagccaggaa
    76081 agcgtgcata cgcgccttca tgactaaaaa cagcagcagt aaaacagacc ctactgctgt
    76141 taaaacaagc gttaatgtag tcactactta tttgcctttt ttaataacct caatggtgct
    76201 tgccacaaca ccttccagcg gttgatcgat atccaccacc agtacatcag tttcgtccgc
    76261 acccggctcc tgcagcgttt caaactgcgt caccaacatt tgggttttaa agaaatggcc
    76321 tttgcgcgct ttcaggcggc tttcaatcac gtcaaaatcg cctttcaggt agatgaaaga
    76381 gagattcgga ttaccttcac gcagcaagtc gcgatagtgt tttttcaatg cagaacagac
    76441 gatcagtgac actttattag tgcgctgcat agcaaacgcg gcgtcgttca gcgcctgcaa
    76501 ccacggtttg cgatcgtcgt cattcagcgg ttcgccagac gccatttttt cgatattgcg
    76561 ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga agttgatgcg ccacttcact
    76621 ggcgaccgca gatttgccgc tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg
    76681 gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat taaaaatgtt acgggtaact
    76741 gttatcggta acattgtcca gccggacaat atcagaagca atatccatcc gtgccttaag
    76801 tgtataagtg tgagctactt caaatttgta ggcttaaata gatccgcccg gtgacaaggt
    76861 gaaacctaaa tctaacattt tcggtgtcac agattcgcca cgaatacgcg ccagcaggcg
    76921 ttcagcgcca atactgccca tccgctcacg cggcgtcagc acgctcgcta accgcggctc
    76981 catcacctgg ccaatgtcat gaccgtggaa accggcaatc gccatatcgt caggaacctt
    77041 caatcccaga cgctgacatt caaacgccgc gccgaccgcc aggtcatcgt tggtacagaa
    77101 cacgccatcc agctgcggat attcccgccg cgcctggcga atcagttcaa taccggaaga
    77161 gtaagaagaa gattgctcaa ccatcacgct atacggcacc agacctgcat ccagcatcgc
    77221 ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg agacgcgcgc cgagataggc
    77281 aatgtggcaa tgcccgcgag caataatggc ggtggtcatc tggcgtgccg cttcaaagtt
    77341 atcaaaaccg acggcgatat cgaggcacgg cgactggctg tccatgagtt ccaccaccgg
    77401 gatccccgcc acttcaatca tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat
    77461 cagtccgtcg atattccatg agagcatgga ttcgagacgt tcttgctcca tttccggttt
    77521 ataaccgtag tgagccagca tggtctgata accgtgcgcg tcggtgacgc tttcgattcc
    77581 gcgtaatact tccgcgaaaa cctggttggt gagagaaggt aacaggacgc caatcgcccg
    77641 gctggtggcg ttagagagga tatcgggcgc acgattggga atatagccca gttcatcaag
    77701 agcggcggca atcttgccgc gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg
    77761 gctgaccgtc attttggtca cgcctacacg gtcagccaca tcctgaagta cgggtctttt
    77821 ctttttcatc gtcctgaagg tacaaaagag atagattctc ttagtttaac acggacgcat
    77881 cagaaccttc ccggggaaaa cagtgcttgt ttacaattat tagatataca ctaaatcaat
    77941 tgcatcgcat tgtgctaacg acgcggcaaa accccggaag cgtgcatcag cacgcgaccg
    78001 gagcgagcga ggaagcctgc aaaaaggcag gcttcaaaga tgccgttgag ttttaaaccg
    78061 gcggcagatc gaacagtaac acttcgctgt cgctatccgc atggatggag attgcctgct
    78121 catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg gtgacattgc
    78181 ctttcaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc gactgctcat
    78241 ctttcagcaa cgcccaacga tacagttcca tatcctgatg cactttcagc gaaccatctc
    78301 gcgcatccgg cgagagcacc agctgtttgc cctgcacggc atcgaagcga cgctgttcat
    78361 aacgcggcgt aatgccattt tcttcaggca tgatccagat ctgatacaga tgcagacgct
    78421 cggtgctgtt tgggttgtac tctgagtgac gaatacccgt acccgcactc ataatctgga
    78481 actcacccgc cggaacctgc tctttattgc ccatgctgtc ctgatgctca acagtacctt
    78541 ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag ccctgccctg
    78601 cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa gcccataaag ttcggatcgt
    78661 aatagttggc aaaagagaaa gtatgccagg agtccagcca gccatgattt gcatgaccac
    78721 gttcatttgc tttgcgtaag tagatcattt gtattcgccc cctgaatgat ttcgatgggc
    78781 ttagtgtgga cccgatctgc ctgggatgat agagggtgaa aattgacccc tctgttcaaa
    78841 aaaattgaac tattcgaggg gccatcaaac ttacttagcg agagttactg tggagggaga
    78901 ggcttgctca aatccgcgtt caaggatttc cagattggta agaacttcag attccttgac
    78961 gtaatttggc gcaccgtggg tgatggtttg atacaacgca tcataaacgc gcccgtaatc
    79021 gcccatctcc ggcttcatct cttctctgac cgtcacgccc tcgtcattga catactccag
    79081 cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc ggcataatat tagccttcag
    79141 gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac cgtgaacgat
    79201 aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt tcaggtcgcc
    79261 ataaaacagt tgcgcttcaa aggtgtcgtc aggattggct ttattacgca ggctgcggat
    79321 gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca tcgtatgcac
    79381 accaaggcca taaaacgcgc catcctgcgg cagcccaggt ttggtttctg ccaccgggcg
    79441 gtaatagtca aaatggcttt ccacttcaac aatctctccc aacttgccac tttcaatcgc
    79501 ttttttcgct gtcaggaagc aggagtcaaa gcgacgattc tgatacggcg tgacggtcag
    79561 ccctttgctt ttcgccaacg caaacagctc cttcgcctgg gcaagcgtcg gggtgaacgg
    79621 tttttcgacc agcacatttt tcccggcttc cagcgcgcgt ttcgcgtact cgaaatggct
    79681 gtccgcgtgg gtgcagacaa caaccagctt aacatcggga tcgtttagta cttcgtcgag
    79741 atcgctggtg aaatggatat gggaataaat gggagcctgt tcttccggct tcgcatggcg
    79801 acgaaaaata tgcgcgacat gccagctatc cttgcggtta agtacatacg gcagatggta
    79861 acgggtggtg cttttgccga agccaataaa ggcgcagttg atgaccatga tttcgtcctt
    79921 tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg gagtgcgcat
    79981 aacgaacaca agcactcccg tggataaatt gagaacgaaa gatcaaaaaa aaagccagca
    80041 cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc aggttctccg
    80101 cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt gtccaacact
    80161 ttttgcaaaa aaatgcattt gactcgcatt tgaaagtcaa tgatgttgaa agggacattt
    80221 accccaaaga ggacaaagga atgagtgaga tagtaatacg ccacgcagaa acacgggatt
    80281 acgaggccat caggcagatt cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc
    80341 ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc atcaagcaac
    80401 tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg caacagcgcc
    80461 cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctctcgttgg aagaaccgcg
    80521 gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag
    80581 atcgcattga actaaccgtg tttgtcgata acgccccggc aattaaggtc tataaaaaat
    80641 acggctttga aattgaaggg actggtaaga agtacgcatt gcgtaatggt gaatatgtcg
    80701 atgcatatta tatggcgcgg gtgaagtaag atagtgccct ttttctgaga tggaaaaagg
    80761 gtgtcattca aaatcgacat accttccttt aaggtattta tttgcccaat acatatattg
    80821 agatttaact cataattact tcctaaagtg taatatttta ttttttaata tatacgccta
    80881 caatttcctg gagtaaataa ataacaatta acaagcataa tattgccatt gataaaatag
    80941 catgccataa aaggactttt cagggatgag taatattgtt tacctgacag taacgggaga
    81001 acaacaagga agcatctccg caggttgtgg gacttctgag tctacaggta atcgttggca
    81061 gagcgggcat gaggatgaaa tatttacatt ctcactctta aataatatta ataatacggg
    81121 gcttggttca cagttccatg gt
//
LOCUS       DAMZII010000002        79750 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZII010000002 DAMZII010000000
VERSION     DAMZII010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.002"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(314..1207)
                     /gene="galF"
                     /locus_tag="POA77_000072"
     CDS             complement(314..1207)
                     /gene="galF"
                     /locus_tag="POA77_000072"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDI6363575.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(1450..2445)
                     /locus_tag="POA77_000073"
     CDS             complement(1450..2445)
                     /locus_tag="POA77_000073"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HDI6363576.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(2603..3997)
                     /gene="wcaM"
                     /locus_tag="POA77_000074"
     CDS             complement(2603..3997)
                     /gene="wcaM"
                     /locus_tag="POA77_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDI6363577.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSLGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4008..5228)
                     /gene="wcaL"
                     /locus_tag="POA77_000075"
     CDS             complement(4008..5228)
                     /gene="wcaL"
                     /locus_tag="POA77_000075"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDI6363578.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGNLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5225..6505)
                     /gene="wcaK"
                     /locus_tag="POA77_000076"
     CDS             complement(5225..6505)
                     /gene="wcaK"
                     /locus_tag="POA77_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDI6363579.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSDYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(6780..8258)
                     /gene="wzxC"
                     /locus_tag="POA77_000077"
     CDS             complement(6780..8258)
                     /gene="wzxC"
                     /locus_tag="POA77_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDI6363580.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLKNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8260..9654)
                     /gene="wcaJ"
                     /locus_tag="POA77_000078"
     CDS             complement(8260..9654)
                     /gene="wcaJ"
                     /locus_tag="POA77_000078"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDI6363581.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(9709..11079)
                     /gene="cpsG"
                     /locus_tag="POA77_000079"
                     /gene_synonym="manB"
     CDS             complement(9709..11079)
                     /gene="cpsG"
                     /locus_tag="POA77_000079"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDI6363582.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGQFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKDKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11272..12708)
                     /gene="cpsB"
                     /locus_tag="POA77_000080"
     CDS             complement(11272..12708)
                     /gene="cpsB"
                     /locus_tag="POA77_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDI6363583.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVNEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(12711..13934)
                     /gene="wcaI"
                     /locus_tag="POA77_000081"
     CDS             complement(12711..13934)
                     /gene="wcaI"
                     /locus_tag="POA77_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDI6363584.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(13931..14410)
                     /gene="gmm"
                     /locus_tag="POA77_000082"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(13931..14410)
                     /gene="gmm"
                     /locus_tag="POA77_000082"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDI6363585.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(14413..15378)
                     /gene="fcl"
                     /locus_tag="POA77_000083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14413..15378)
                     /gene="fcl"
                     /locus_tag="POA77_000083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDI6363586.1"
                     /translation="MSKQRVFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(15381..16502)
                     /gene="gmd"
                     /locus_tag="POA77_000084"
     CDS             complement(15381..16502)
                     /gene="gmd"
                     /locus_tag="POA77_000084"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDI6363587.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(16528..17076)
                     /gene="wcaF"
                     /locus_tag="POA77_000085"
     CDS             complement(16528..17076)
                     /gene="wcaF"
                     /locus_tag="POA77_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDI6363588.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17092..17838)
                     /gene="wcaE"
                     /locus_tag="POA77_000086"
     CDS             complement(17092..17838)
                     /gene="wcaE"
                     /locus_tag="POA77_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDI6363589.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(17849..19066)
                     /gene="wcaD"
                     /locus_tag="POA77_000087"
     CDS             complement(17849..19066)
                     /gene="wcaD"
                     /locus_tag="POA77_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDI6363590.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19041..20258)
                     /gene="wcaC"
                     /locus_tag="POA77_000088"
     CDS             complement(19041..20258)
                     /gene="wcaC"
                     /locus_tag="POA77_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDI6363591.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20255..20743)
                     /gene="wcaB"
                     /locus_tag="POA77_000089"
     CDS             complement(20255..20743)
                     /gene="wcaB"
                     /locus_tag="POA77_000089"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDI6363592.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(20746..21585)
                     /gene="wcaA"
                     /locus_tag="POA77_000090"
     CDS             complement(20746..21585)
                     /gene="wcaA"
                     /locus_tag="POA77_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDI6363593.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(21678..23840)
                     /gene="wzc"
                     /locus_tag="POA77_000091"
     CDS             complement(21678..23840)
                     /gene="wzc"
                     /locus_tag="POA77_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDI6363594.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(23843..24286)
                     /gene="wzb"
                     /locus_tag="POA77_000092"
     CDS             complement(23843..24286)
                     /gene="wzb"
                     /locus_tag="POA77_000092"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDI6363595.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(24292..25431)
                     /gene="wza"
                     /locus_tag="POA77_000093"
     CDS             complement(24292..25431)
                     /gene="wza"
                     /locus_tag="POA77_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDI6363596.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            25740..25889
                     /locus_tag="POA77_000094"
     CDS             25740..25889
                     /locus_tag="POA77_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363597.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            26090..27673
                     /locus_tag="POA77_000095"
     CDS             26090..27673
                     /locus_tag="POA77_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6363598.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(27946..29799)
                     /gene="asmA"
                     /locus_tag="POA77_000096"
     CDS             complement(27946..29799)
                     /gene="asmA"
                     /locus_tag="POA77_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDI6363599.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(29821..30402)
                     /gene="dcd"
                     /locus_tag="POA77_000097"
     CDS             complement(29821..30402)
                     /gene="dcd"
                     /locus_tag="POA77_000097"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDI6363600.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(30494..31135)
                     /gene="udk"
                     /locus_tag="POA77_000098"
     CDS             complement(30494..31135)
                     /gene="udk"
                     /locus_tag="POA77_000098"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI6363601.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            31453..34770
                     /locus_tag="POA77_000099"
     CDS             31453..34770
                     /locus_tag="POA77_000099"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6363602.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     LGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(34809..35666)
                     /gene="alkA"
                     /locus_tag="POA77_000100"
     CDS             complement(34809..35666)
                     /gene="alkA"
                     /locus_tag="POA77_000100"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDI6363603.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            35800..37152
                     /gene="yegD"
                     /locus_tag="POA77_000101"
     CDS             35800..37152
                     /gene="yegD"
                     /locus_tag="POA77_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6363604.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37164..39104)
                     /gene="yegI"
                     /locus_tag="POA77_000102"
     CDS             complement(37164..39104)
                     /gene="yegI"
                     /locus_tag="POA77_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDI6363605.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     SFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(39101..39862)
                     /locus_tag="POA77_000103"
     CDS             complement(39101..39862)
                     /locus_tag="POA77_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDI6363606.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(39859..40518)
                     /locus_tag="POA77_000104"
     CDS             complement(39859..40518)
                     /locus_tag="POA77_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6363607.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(40739..40798)
                     /gene="ibsA"
                     /locus_tag="POA77_000105"
     CDS             complement(40739..40798)
                     /gene="ibsA"
                     /locus_tag="POA77_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDI6363608.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41071..41127)
                     /locus_tag="POA77_000106"
     CDS             complement(41071..41127)
                     /locus_tag="POA77_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6363609.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(41399..41455)
                     /locus_tag="POA77_000107"
     CDS             complement(41399..41455)
                     /locus_tag="POA77_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6363610.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            41734..42981
                     /gene="mdtA"
                     /locus_tag="POA77_000108"
     CDS             41734..42981
                     /gene="mdtA"
                     /locus_tag="POA77_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDI6363611.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARLNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            42981..46103
                     /gene="mdtB"
                     /locus_tag="POA77_000109"
     CDS             42981..46103
                     /gene="mdtB"
                     /locus_tag="POA77_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDI6363612.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46104..49181
                     /gene="mdtC"
                     /locus_tag="POA77_000110"
     CDS             46104..49181
                     /gene="mdtC"
                     /locus_tag="POA77_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDI6363613.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49182..50597
                     /locus_tag="POA77_000111"
     CDS             49182..50597
                     /locus_tag="POA77_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6363614.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGIKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLLLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQAVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50594..51997
                     /gene="baeS"
                     /locus_tag="POA77_000112"
     CDS             50594..51997
                     /gene="baeS"
                     /locus_tag="POA77_000112"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDI6363615.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAGQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            51994..52716
                     /gene="baeR"
                     /locus_tag="POA77_000113"
     CDS             51994..52716
                     /gene="baeR"
                     /locus_tag="POA77_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDI6363616.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            52907..53239
                     /locus_tag="POA77_000114"
     CDS             52907..53239
                     /locus_tag="POA77_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDI6363617.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            53448..53744
                     /locus_tag="POA77_000115"
     CDS             53448..53744
                     /locus_tag="POA77_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDI6363618.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            53746..54042
                     /locus_tag="POA77_000116"
     CDS             53746..54042
                     /locus_tag="POA77_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6363619.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            54145..55506
                     /gene="yegQ"
                     /locus_tag="POA77_000117"
     CDS             54145..55506
                     /gene="yegQ"
                     /locus_tag="POA77_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDI6363620.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(55836..56153)
                     /locus_tag="POA77_000118"
     CDS             complement(55836..56153)
                     /locus_tag="POA77_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363621.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            56568..57467
                     /gene="yegS"
                     /locus_tag="POA77_000119"
     CDS             56568..57467
                     /gene="yegS"
                     /locus_tag="POA77_000119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDI6363622.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(57549..58322)
                     /locus_tag="POA77_000120"
     CDS             complement(57549..58322)
                     /locus_tag="POA77_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6363623.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(58428..59468)
                     /gene="gatD"
                     /locus_tag="POA77_000121"
     CDS             complement(58428..59468)
                     /gene="gatD"
                     /locus_tag="POA77_000121"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6363624.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPDDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(59516..60871)
                     /locus_tag="POA77_000122"
     CDS             complement(59516..60871)
                     /locus_tag="POA77_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HDI6363625.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(60875..61159)
                     /gene="gatB"
                     /locus_tag="POA77_000123"
     CDS             complement(60875..61159)
                     /gene="gatB"
                     /locus_tag="POA77_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDI6363626.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(61190..61642)
                     /gene="gatA"
                     /locus_tag="POA77_000124"
     CDS             complement(61190..61642)
                     /gene="gatA"
                     /locus_tag="POA77_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDI6363627.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(61652..62914)
                     /gene="gatZ"
                     /locus_tag="POA77_000125"
     CDS             complement(61652..62914)
                     /gene="gatZ"
                     /locus_tag="POA77_000125"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDI6363628.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLSKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(62943..63797)
                     /gene="gatY"
                     /locus_tag="POA77_000126"
     CDS             complement(62943..63797)
                     /gene="gatY"
                     /locus_tag="POA77_000126"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDI6363629.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALEFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(64105..65157)
                     /gene="fbaB"
                     /locus_tag="POA77_000127"
     CDS             complement(64105..65157)
                     /gene="fbaB"
                     /locus_tag="POA77_000127"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDI6363630.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            65414..66691
                     /locus_tag="POA77_000128"
     CDS             65414..66691
                     /locus_tag="POA77_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HDI6363631.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            66688..67692
                     /locus_tag="POA77_000129"
     CDS             66688..67692
                     /locus_tag="POA77_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI6363632.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            67689..68654
                     /locus_tag="POA77_000130"
     CDS             67689..68654
                     /locus_tag="POA77_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI6363633.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(68628..69374)
                     /locus_tag="POA77_000131"
     CDS             complement(68628..69374)
                     /locus_tag="POA77_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6363634.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(69426..70244)
                     /locus_tag="POA77_000132"
     CDS             complement(69426..70244)
                     /locus_tag="POA77_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDI6363635.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(70309..71109)
                     /gene="thiD"
                     /locus_tag="POA77_000133"
     CDS             complement(70309..71109)
                     /gene="thiD"
                     /locus_tag="POA77_000133"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6363636.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHYHAWW"
     gene            complement(71106..71894)
                     /gene="thiM"
                     /locus_tag="POA77_000134"
     CDS             complement(71106..71894)
                     /gene="thiM"
                     /locus_tag="POA77_000134"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI6363637.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(71929..72025)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(72231..72413)
                     /locus_tag="POA77_000135"
     CDS             complement(72231..72413)
                     /locus_tag="POA77_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363638.1"
                     /translation="MNNDKANHPKHFQYLGRMVTVYPTYIIIDGIKISRSRLSFAFQL
                     ELAKALRLHEESVCRK"
     gene            73377..73943
                     /locus_tag="POA77_000136"
     CDS             73377..73943
                     /locus_tag="POA77_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6363639.1"
                     /translation="MNFVEGCKSVQEVKAVATELLKQGKGLTLYRDAWLLGYELMLRV
                     SDLRSIRYDDIKGDHLVLSQQKTGESVRVRLTDTAKRIIAARREANPDHVYLLQLDSH
                     RSKGKPVSRSKLHEEISYAGEKLGLNLSTHSMRKSKPTIGYDNGEDIAVISKALGHKS
                     LSSTLHYIGATQRKVDDFSNKYSIEDLL"
     gene            complement(74097..74387)
                     /locus_tag="POA77_000137"
                     /pseudo
     CDS             complement(74097..74387)
                     /locus_tag="POA77_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(74351..75608)
                     /locus_tag="POA77_000138"
                     /pseudo
     CDS             complement(74351..75608)
                     /locus_tag="POA77_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            complement(76151..76489)
                     /locus_tag="POA77_000139"
     CDS             complement(76151..76489)
                     /locus_tag="POA77_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363640.1"
                     /translation="MEAYKTLLAQLEQAKALERQKATQAEVTKSAAALSELEALIDQY
                     KETLSEGPESGPARNVKSRGIALCHKDQGFSANGRSVSLLTKSVEGLRRVNINKASRP
                     LMATKLEGAE"
     gene            complement(76626..77198)
                     /locus_tag="POA77_000140"
     CDS             complement(76626..77198)
                     /locus_tag="POA77_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363641.1"
                     /translation="MSIITTEVKALTPEEEEKIAALSLKLATSKPRPLMDEKKLTTDQ
                     IVQIKRACVMGHSAKAICSAFNVSLAYALKMKREYNPVKYQKAVLTLPEKAVMIQQMK
                     ADNLPDQMIGEMLGINVKTVQTLTRVAPARYLVDQMLPYDQVLANLRAPRYVANPVYK
                     LGTSMTRVRKIISAGRKELRTVIISSKRAA"
     gene            complement(77286..77912)
                     /locus_tag="POA77_000141"
     CDS             complement(77286..77912)
                     /locus_tag="POA77_000141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363642.1"
                     /translation="MPATASKPFTAVCHELADIEKINGKVFTMNMVYTYALIEGYQTN
                     GQTAYFSQELLSRRLGLTDRSVRNLLREMVEMGLINKTGQIGGKTCHYTVNPITPEMV
                     GTVEAEAPQEQPQKATAEREEMQEPNHTAEPVEAPEAPEIVPATPGPTTPITLMTVYS
                     EKVETVGEGLVQADPQPGETASVVPAVTPYPRGEIKEPSRYWDLDPAF"
     gene            complement(77893..78063)
                     /locus_tag="POA77_000142"
     CDS             complement(77893..78063)
                     /locus_tag="POA77_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363643.1"
                     /translation="MKIDFPKEQADQLYALVRAGGGAPHTIVKEALAAYLKNHPLLVA
                     SAEGKQNASNSK"
     gene            complement(78060..78320)
                     /locus_tag="POA77_000143"
     CDS             complement(78060..78320)
                     /locus_tag="POA77_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363644.1"
                     /translation="MLHQFTDLSESRLVNGYASQIINSIRDDDSAPGLYDDIYSMLQQ
                     VEPSRRVTLGNPAITAPAKWWGEFFGLSLSADDIDELKEINL"
     gene            complement(78346..78771)
                     /locus_tag="POA77_000144"
     CDS             complement(78346..78771)
                     /locus_tag="POA77_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363645.1"
                     /translation="MSIYLDVEKMVERVDQRDLTRKTLTETRSRMKAAGRMREVEAIT
                     QALELTKSSASGVMRQSQRIAAKVTEMDAEKALEMKATVALFASKPTDLQASIVLAFQ
                     SLFEAKGVPMEYDEVMAFIMLNAADHFERITGELPVIVH"
BASE COUNT        18804 a        21125 c        20131 g        19690 t
ORIGIN      
        1 taactctcgt aatttccacg ctaccgcccc tggctttaca gctaccagag cactgcatac
       61 atgcctacga tgtgacgagc gttacccact cgcgcttaac tcgaaaaatt caaacgctaa
      121 ttgtcttacc aatccgttct ggaaacaagg aaaatcctgg aaaactttga ctaaaatcct
      181 attgctaact cgttgttatt ctgattgttt atataaaaca acggcaggaa tattcgcaac
      241 aaattacttt tgccacgaat cctcactgcc gttataattt tcttatcaac cgttacatcc
      301 ggtcagattt tcattattcg cttaacagct tctcaatgcc tttacggaac ttcgcccctt
      361 ctttcaggtt gcgcagtcca tacttcacaa acgcctgcat atagcccatt tttttaccgc
      421 agtcgtagct gtcaccagtc atcagcattg catcaacgga ctgttttttc gccagttcgg
      481 caatagcatc agtcagctga atacgtcccc atgcaccagg ctgagtgcgc tccagttccg
      541 gccaaatatc ggcagaaagc acataacgac ctacggccat gatgtctgag tccagcgtct
      601 gcggctgatc cggtttttcg ataaattcaa caatgcggct gactttgcct tcacggtcca
      661 gcggctcttt ggtctggatg acagagtatt cagagaggtc acccggcata cgttttgcca
      721 gcacctggct gcggcccgtt tcattgaaac gcgcaatcat ggcagcaagg ttgtagcgca
      781 gcgggtcggc gctggcatca tcgataacaa cgtccggcag caccacgaca aatgggttgt
      841 cgccaatggc ggggcgtgca cacaaaatgg agtggcccaa acctaaaggt tcaccctgac
      901 gcacgttcat aatggtcacg cccggcggac agatagattg tacttccgcc aacagttgac
      961 gcttcacgcg ctgttccagg agagattcta attcataaga ggtgtcgaag tggttttcga
     1021 ctgcgttctt ggacgcgtga gttaccagga ggatttcttt gatccctgca gccacaatct
     1081 cgtcaacaat gtactgaatc attggcttgt cgacgatcgg tagcatctct ttgggaatcg
     1141 ccttagtggc aggcaacata tgcatcccaa gacctgctac cggtataact gcttttaaat
     1201 tcgtcattct tttcctacct ctaagtggct gatagtgcgt aaattattgc cataggttag
     1261 ccaaacgtta tggctatata ccaaagctta gttccgcata actttgctta aaccttagga
     1321 taacactaca caccatcagc atctgggtta ctcggattac tcggaattcc gcatactgat
     1381 aatttaatca gtacctcttt ccgaataatc gtagtccaac ctggtccttt tttctctgac
     1441 tcgtctgcat tactcagaaa caaacgttat gtcgtctttt ttggcatgaa cgaattcata
     1501 ctgcagagtt cgatccagac cttgcgacag cgtatacggt gccacaaaac ctgaagaatg
     1561 cactttcgtt gcgtcaaact gtgttgttgc gcagaatttt ttcacgcgca cagagctgac
     1621 agcgtatttt ttgcccgtaa ttttgctcag gatatcaaag caatatccac ccaacattcc
     1681 tagtgggtaa ggcaagtgca tagaagggat ctttttgttc agactttgtt caacttcagc
     1741 aaccaactgg ttcatgttca gatctggctt atcaacgtaa ttataaacct cataacctgc
     1801 ggcaacattc ttcagtttgt acttgataaa ctcaacaatg tttccaacat aagccatgga
     1861 cttatagtta gtccctgcgc ccaccatcat aaacttgccg ccagcgatct gtttcagcaa
     1921 gttatagacg ttaccgcggt tgcgttcacc gaagataacg gtaggacgga tgatggttaa
     1981 tgaacgttct gttggtgctt tgttatacca ttcacgcagc acttcctctg cctgccactt
     2041 acttttgccg tagtggttga aagggtcgtg tggatggttt tcgtcagggt tgtgtttgtt
     2101 caaaccataa acagcaacgg aactggtaaa gatgatattt ttaacgccat ttttttccat
     2161 ggccgccagc acattgcggg taccctgaac gttgacatca taatagagag aagtagggct
     2221 gacgtcatcg cggtgttccg ctgccagtag tacaacagtg tcaaaaccgg ctaacgcctg
     2281 gtcgagtgcc tgttgatcac gaacatcgcc aatctgtgtg atttctggat aaaagtggct
     2341 ctgctgtttg tccaggttct tgatattaaa gtcagcaatt gccgtttcaa gtagtcgggt
     2401 tcctacgaat ccggaagctc ctatgagcaa aacgttatcg ttcataaatc actttagtct
     2461 ggttgttacg taagaaacac aagataaaga tgagtacctt ccctgagtag tcaatgctgc
     2521 ccagccccag ctttaacagt tagtgtgagg attataatct tttagaacat tatatccagt
     2581 aagtttatga atggtcgcaa atctactccc tccgttccgg caatctaaag ttaatctttt
     2641 ccacattaac aatatggtga ttaatcctgt cgatatcgac ggagctttgt cctctttcat
     2701 tcaccgcatg aacatttgca agagacagga gtgtttcttt tttcgccata aagacgccgc
     2761 gaacgtcttt gcgcatgtcg aagttcatgc tcaatgcggg tccaagagag gattcctgca
     2821 tcacattaat attacgcata aaaagatgtt gcggtttgtt gtgtaactcc agcgacgcac
     2881 gcttcatctc aatgttagtc agcgccacaa aggagacagc attcccggcg gagatttgga
     2941 tgccgcgcaa tttataaaca agatgggtgt tatccagttg aatattattc actcggaaat
     3001 tttgcggtat cgagagatat ttgcctttaa ttaccccata gccgattaac atcccggcgc
     3061 tattaatcat ttcaatatta tcaatcacga aattgtcaca accgtaaata gcaactgtcg
     3121 cgttatcaat gcctgctttc ttactgaaat ccggcgtgat attgcgagct ttgatattac
     3181 gaataacaaa atgtttgcca ttttcaacat ggatcaactg ccgacaatcc gatcccgtga
     3241 tattcgccac gacaaagttt ttcactgcct ggtcttccgg gtagttatta tcgtaggtac
     3301 tccccgcaag gcctatgccg atgccccagt tgattttgcc attagtacag ttgatgcgct
     3361 cgatgacatg gtcggagatc aaaatatcac tgtcgttaat tgccacgttc cattcaatgg
     3421 cgtcgccttg taagtcgctg aacttacagt tggtgatgtt ggcaccgata atctggttat
     3481 gaaatccctg gcgtaagatg gcgtaattag cgtggctaac ggtcaggtta tcgatggtca
     3541 ggttgcgcat gacccgtttg tttttgccgc cgatatagat ctgcgtcacc gggccaaagc
     3601 cgctcatcgt cagccctttg atggtgcagt cagagccacg cacatccagg gtgatgttat
     3661 gcatactgcc gccctcctcc cctgtcacct ggctgccgtc ctgtaagaca aatcgccctc
     3721 tgccgttgcc gcgcaggctt ccaaggatgt gtaacgtttt acctgcagga atgaagatgc
     3781 cggtgttgat attgtcacaa accaatccgg caggcacgac gaccgtttgc ccttcgctga
     3841 aggcttgttt aaatgaggcg atccagtcgt gagggttgta gtcgttaatg ttaacgcttt
     3901 gtcgggcggg aagtgcgcga gcgaaagggg tatggaggaa ggcaagcgcc gagcttgccg
     3961 tcaggaaggt gcgtcgggag agttttttaa atggcatacg ttctcctcta taaagcctgc
     4021 agcaagctgg cgagttctcg attgatcacc tgctggttaa aatcgtgttc gactttttcg
     4081 cgcgcacgtt tgacgaccgg agccagttcg tcggtgtcca gttggctaaa cgccgccagt
     4141 cgttgtgcca gtgcgcgagc atcgttctca ggcaccagcc agccggattt gtcagcctcc
     4201 accagttccg gtattccgct atgcagagta gaaaccaccg gaatgccgac cgccatcgct
     4261 tccattagcg ccactggaat gccttccata tcgccatccg cgcctgtaac cgacggcaac
     4321 aggaagacat ccgcgtcgtc gagcatcgct tttacttcat ggctcggttt aaagcccggc
     4381 atctccacca catcttccag ttgatattgt tcgatgagcg tgcgcaggcg tcgttcccac
     4441 gggccaatgc cgaggatgcg atagcgaaat gccatgccct gctctttcaa ctgacggcag
     4501 gcttcaatcg caacatgcag gccttttttc tcggttaagc gtgcgacgga aatgatttcc
     4561 agcggcgttg cgggcgcttt caccggacgc gggctaaaac gcgtcatgtc cacgcccata
     4621 cgcgatacgg caattttttc cctcgggcag cccatttttt gcagccttcc ggcccacaga
     4681 ttacttatag gtaacatcag gttgccacgg cgaaacagtt gctgatattc gggagtgtaa
     4741 tggttgagca cttcccgact ggaaatatca atgccgtgga aaatagtggc aattttgccg
     4801 cgaatgacac ccagttcgcg tagttttgct gcggttaccc ccgcagggcc aaaatgagcg
     4861 ataaagacat cggcatgaaa cggtgttgcg acctgaccac aaatggcaga caaaatcagg
     4921 ttccgcgact cggcgccata gcgtttgagg ttgagcgcct gccaggtatt tttacgatga
     4981 atgccacgta aggtctggct ggcgcggtgg cgcagcttcg ctactttgcc cgcaggttcg
     5041 tcctgtaacc aacgggttct tgcggcaagg ttgtatttcg tccatgccgc gtgggtgttc
     5101 tgggtgtcgc ctttttgcag cgcaacaatc tccacctcga atcccatatc aataaacgcg
     5161 gtaatttggt taagaacgaa ggtttccgac gacagcggaa atttcagtaa aaagaagccg
     5221 accttcattt cacctccccg atgcgttcaa gcacagattg caccatctgc attcccgtct
     5281 gacgttcgcg actgacggct tcgttaagtc gagcgttcag caccggaagc tggcctaagg
     5341 tatccgcaac catcgcttgc aggctgccgt ccagtaaatg acggatatca attgccatct
     5401 ccggtagtcc cagctgctgc atgatcccgg cggatttatg ttcatagttg atggcaatcg
     5461 ccggagtagc aaagttcatt gagataatag cggagtgcag acgcgtaccg acggtgagtt
     5521 cacaggcacc gagaattttg cccatttcca gatcgttgag ttcatccatc actacgtgat
     5581 aacgggcagg atcgctgatg tgctggcgca ggttgagcgc caccatgcgg tcgtctttgt
     5641 tatagctgtc gatgcccgta caggtggaaa gcgcaatcac ctgataccct tcgtcgagaa
     5701 tgcgattgac caccccggca aaggcttttt catacgcttg ctgagtggtg ccgagacgtt
     5761 tgtcgaacgg tgccaattcg cgcagggtaa ttgctaccgt tttctgttgt gcggcaacgt
     5821 ccagccagtg ttgaacggca tagctggcgg tgaagtcttc ggtgtggtga tcgaccagcc
     5881 acgcggtatc aacgccatgt tcaacttttg cggtagtgat attgctgcgt ttcattagat
     5941 caaggctgac cgattcgcgc aggatcagcg cgtcacagtg accaaaaacg tagttcgcca
     6001 gttggttaaa ttgctcatcc tggaagggac cgacgctgtg accaatcata aacagcggct
     6061 ttttcgccat aaacgtgcaa agtgcatgtt caaactgcgg cacgccgtag agatcgacaa
     6121 aaaacgatcc gccgacctga ataatggcgt cgtaatctga cagcaggcgc acgaagtcgg
     6181 tgaatccctg ggcgatggcg atattacgca gcttgccagt gtcggtgacg cgtgagagca
     6241 atacctgatg ctggtagcgg cgacggagga cttttttaac gcgcccgaca acgcccgccg
     6301 cgctgttgtg ttgtttcatt tgcaggaaca gcggatcgcc cattaccggg cggttgagca
     6361 gccaggaaga actgaccgga tagcggctca tcacgtccac ttcggcgtgt ggattgagga
     6421 tgttgatggc atcaagtaag ccgcgcagga tggcgctgtc gccacgattg ccgcaagtgt
     6481 ggttgcccag aataagtaat ttcatttttt cctcataaat ttgatgccag gtgggctgtg
     6541 tttgttttgt tcgttgccgc tttttgcctg atgcgacgct gacgcgtctt atcaggccta
     6601 caagacccga gcacagaacc gtaggccgga taaggcgttc acgccgcatc cggcattcag
     6661 tgcctgatgc gacgctgacg cgtcttatca ggcctacagg tcccgagcac agaaccgtag
     6721 gatggataag gcgttttacg ccgcatccgg caaccgttgt cggaaccgaa aacagcaact
     6781 caccccgccc gtaaaagcat tttcattttt tcgctgcgac aaaactgacg cttcacttcc
     6841 accaccagcg gatggcgcga cagcacaatc atcaccacaa acgccagcac gcccgcggct
     6901 atttgcaccg ccagcagcat ccccagcgcc agttgccctt tcagcacaat gcccagcgca
     6961 taactgacca ccagcgtcgg caacgagaga taaaacggca gccacaaact caggatgtac
     7021 tggcgataac tggaaccaag aaccggttta atcatcacga agtaactcag aatggtgttg
     7081 ataatctgta ccaacaggaa gccgagcgtg acgccaattg cgcccgccat ctgcccacca
     7141 ataacaatcg ccggaataaa cagaaaggtt ttgaatacgt tgaatttaaa gctgatatcg
     7201 acccgcgctt tcgccatcag cagcgaacca atcgggttac ctacggaacg cagcagaccc
     7261 accacacaca gcaattgcag caccggaata atgctgttcc acttctcacc aaagaccagc
     7321 ggtacaaagt tattcgacac caccatcagc cccagcagcg ccggaaagtt gataatcccc
     7381 actaccgaca gcagcttgta gaagttaacg cgcagctttt cggtatcgtc ctgaattttg
     7441 gcgaatgccg gaaacaatac gcgggtgatg attgggttca gcttcatcgg tggcacaacg
     7501 gccacgttgt acgccaggtt gtatccccct gccacgcccg cgccgagaat acgcgccagc
     7561 accagcgttg aaaggttggt attgagatag ttgatgatgc tgtccgccgt cagccaggca
     7621 ccaaagcgta agttcggtgc taccgacgcc agcgagaagt gcagaccggg tcgataaatt
     7681 ttgcggccaa agtagccaaa cagcagcgtt cttaccgcac tattgaccag ataaccgagg
     7741 atcgcggtca tcgccagcgg ccagaaatgg gcgctaacca ccgtaaaagt aaagcccgcc
     7801 agcaccgcgc tggtttcgat catgccgatt ttgttgaact ccagttcctt ttgcatcaac
     7861 gcgcggaact gctgcccgtg ggggatcacc acaaacgcca gcgacaatgt tttaatcaac
     7921 ggtgccagat ccggattctt cagcacgtcg ccgatgagat cactcaacaa aaacaccgcc
     7981 acgcacacca caagccccag cccaacgttc agccagtaca gcgtggtgag ttcaaggtgg
     8041 ctgatttctt ttcgctgaat aatcgagtta gcgataccga agtcagaaag cgtatctgcc
     8101 agcgcaatga tcaccagcga cacggtaagc aaaccgaact ggtggttgtc gataatccgc
     8161 gccagcacgg tcatctgcac cagcccgagg ccgatgatga tcaccgtagc aatcgccgac
     8221 cacttcgcgc cgctgatggt cttttcacgt aagctcatat caatatgccg ctttgttaac
     8281 gaagcccttg aataccgtca ggaaaacgat tttgatatcg aaccagacgc tccattcgcg
     8341 gatgtactca aggtcgaact cgacgcgttt ttccattttc tccagcgtgt cggtttcgcc
     8401 acgccagccg ttaatctgcg cccagccggt aatgcccggt ttcaccttat ggcgcagcat
     8461 gtagccttca atgagctgtc gatactgttc gttatgcgcc accgcgtgcg gacgtggacc
     8521 gacaatcgac atccccccgg tcagcacatt gataaactgc ggcagttcat ccagcgaggt
     8581 gcggcgcaga aagttcccca ctttggtgac gcgcggatcg ttctgcgtcg cctgggtcac
     8641 cactttgtcg ttctccatca ctttcatgga acgaaacttc cacactttga tcggcttgcc
     8701 atccatgcca tagcgagtct ggcggaaaat aaccggccct ggtgaactga gtttcaccgc
     8761 cagcgcaata cagcacagca ccggggagat cagcagcaga ataagcgtcg ccagcacaat
     8821 gtcttccgca cgttttatca ggcggttaac cccggaaagc ggcgtgtcat acagcggcac
     8881 caccggaacg ccgttcatct cttcgagacg tgaatggaga atgttgaagg taaagacgtc
     8941 ggggatcagc agcaccgaac aggtggtatc cgccagttga tggaccagtt ttttcactcg
     9001 tgcgccgtcg cacatttgca tcgcgatata gacgttatga atcttgcccg ctttcgcgtc
     9061 ctcgaccagc tgttgcagat tgcccgccca gtcgttagaa acgccgcccg gtttcgggtc
     9121 gtggtaaacg cccaccactt caaaccctaa ccacggctga ttacggaagc tctccatcag
     9181 catttgtccg gcggctaaat cccccgccac cgcgaccatg cgcttgttat agccatgatt
     9241 acgcagccag cccgccccaa tgcgaataca tgaacggcaa accaccagtc cgatactggt
     9301 cagcccatac cacgccagcc agattttcag ttgcgtgtcg aaatcattat tgaacgccac
     9361 cagtccggcg ctgaaaatca cgcttaaggt ccagttttgt agcaacaggg caaattctgt
     9421 cgctgcccga acaccgcgcc atgagcgata aaaatcggtg atgccaccca gcatctggaa
     9481 taccaccagc gtaatcagcg ccaccaacag gtgcatgtag aggaatgaca gtccgctgac
     9541 ttcgcaaacc agccatagcc cggcaaacat gatggtgata tctgaaaagc gttgcaccat
     9601 agagattaac gatgcattgg ttttcgctcg ctcgcgcttt tttagatttg tcatcgttgt
     9661 tcctgttatt agccccttac ccgcaatggg gtaagggagg atccgacatt actcgttcag
     9721 caacgtcagc agagttcgcg ttcgcgcttc catcagcggc acatcaccgc gcgattcaac
     9781 attcaaccgc accaccggtt cggtgttgga ggagcgcagg ttaaagcgcc agtcggcaaa
     9841 ggtcatgctg atgccatcgg tgcgatccac cgccagcgcc tcacggctaa aatgctgttc
     9901 cacgcgatta atcgcctcaa cgggttgcgc cagtttgctg ttgatctcac cgcttgccgg
     9961 aaacgcagcc atccggtcgc gtaccagttc gcccagcgtt ttatctttca ggcacaccag
    10021 ttcggcgacc agcagccacg ggatcatgcc gctgtcgcag taagcgaaat cacggaagta
    10081 atggtgggcg ctcatttcgc caccatagat ggcgtcttcc ttgcgcatac gttctttaat
    10141 aaaggcgtgt ccggttttcg acattaccgg cgtgccacct gcggcagtca ccacatcaac
    10201 ggtgttccag gagagacgtg gatcgtggat gatcttcgcg ccgggatttt tttcgaggaa
    10261 tgcttctgcc aacaggccga caatgtagta gccctcaata aactgccctt tttcgtcaaa
    10321 caggaagcag cggtcaaaat cgccatcaaa agcaatgccc atatccgcgc cgtgtttgat
    10381 gaccgcattg cgggtgtcgt cgcggcattc cggcagtagt gggttaggaa taccgttggg
    10441 gaaattgccg tccggtgtgt tgtgcacttt gattaattcc acgggcgcgc cgagggcttt
    10501 aaagcgggct tcaatggcgt ccaccaccgg acccgctgcg ccattcccgg agttgatcac
    10561 cagcttgagc ggcgtgagat ttttgacatt gatataaccg aacaggtgat caacgtaagc
    10621 gtcacgcagg ttgatttgct gatagcgacc gcgtttggtt tcatcgacgg gaggaaagtc
    10681 gttggcttca gccagacgct ggacgtcgcg cagtccggta tcaccgctga tcgggcgagc
    10741 cccctcgcgc accagcttca tgccgttgta atccatcgga ttatggctgg cagtaacttc
    10801 aatgccgcca tccacgccga gatggaacgt ggcgaaatag atctcttcgg tgccggacat
    10861 accgatatcc agcacgtcaa cgcccgcatc ctgtaaacct tttgccagcg ccagttttaa
    10921 ggtttcgctg gtgaggcgga catcaccgcc taacacaatg gttttcggtt tgagaaattg
    10981 gccataggca cgcccaatac gccaggcaat atcttcattc agttcttcgc ctaatttccc
    11041 gcgaatatca taggctttaa agcaggttaa ttttttcatg tcgttaccct ttttcaggca
    11101 atagttggcc ctgaccgaaa cggccaattt tgtttttgtc gttatttaca tctcggtttc
    11161 gcattcattg cctgatgcga cgctgccgcg tcttatcagg cctacaaacc cgaaccgtag
    11221 gtcggataag gcgttcacgc cgcatccgac aaacagctcc tgatgcgacg tttacacccg
    11281 tccgtagcga tccgcgaaac gcaccacatc atcctcttcg agataagagc cggagcgcac
    11341 ttcaattaaa tcgagcggaa ttttccccgg gttttccagg cagtgcgtcg cccccagcgg
    11401 aatataaatg gactcgtttt caccaagcag tttgatatca ccatcaatgg tgacttttgc
    11461 cgttcccgcg acaaccaccc agtgttccgc gcggtgatga tgcatctgta ccgacaagcc
    11521 ttcgcccggt ttcacggtga tgcgtttcac ctggtagcgg tcgcctgcgt cgatagagtc
    11581 atatttgccc cacggacgat acacttcgcg atgcacccga tgctcatggc gaccatcggc
    11641 tttgatctgc tcgaccactt ttttcacatc ctgcaccgcg ttacggtcgg caatcagcac
    11701 cgcatctttg gtctgcacca ctaccagatc tttcaccccg acggtggtga ccaggccaga
    11761 ttcggcgtac acatagctgt tttcagtttt gtgattaatc acatcaccgt ggcaaacgtt
    11821 gccctcggcg gtgtgggcgc tgatctccca taatgaagac caggaaccga catcgctcca
    11881 gcccgcatcc atcggcacca caacggcatc tgccgtacgt tccatgaccg cgtaatccac
    11941 cgactcttcc gggcaggcga gaaacgcttc ttcattcacg cgaataaaat cgagatccgg
    12001 atcgacggcg ctcatcgctt tttcacaggc gtcgagaata tccgggcgat attttttcag
    12061 ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg ctgttccagt aatattcgcc
    12121 gcttgccaca taggcctggg cggtttccag attcggtttt tcgacaaact gcgccacttc
    12181 aaaggccacc gtatcctgct cacccgcaga cacttcaccg cgacgaatat agccataacc
    12241 ggtttctggt agatccggca caatgccaaa ggtcaccagc ttgcccgctt cggcatatgg
    12301 catggcatta cgcacggcgg cacggaacgc gtcttcatcg gcaatcacat gatccgccgc
    12361 caataccagc attaacgggt cgctctccgg gctatgacgt tttgccgcca gcgccgccaa
    12421 cgcaatggca ggtgcagtgt tacgccctgc aggttcgaga ataatgttct cggtgagttt
    12481 gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc tgctcattgc aaatcaccac
    12541 cgggctttcg cactccacgc cgttcaggcg gcagatggtg gtttgcagca tggtgagatc
    12601 gcctttcagg cataaaaact gcttggggta aagtacgcgg gaaagcggcc ataaacggct
    12661 accggagcca cctgccatca caactggata gagtttcgac tgcgccataa ttatccccga
    12721 atatcattta taaattgacg taacacgttc tctttatcga gcgtgcgttc ggcatattca
    12781 cgtgccaccg tgttgtgttt gggcagcagg agcgcctgac ggatccccgc caccagcgcc
    12841 tcgaccgatt ccggttctac gcaaaccgca atgcccggaa aggtttcgca aagctgtccc
    12901 agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc ctactgccag aatattggtc
    12961 agtttcgacg gcaatacggc atctgccgcg ccgcgttttt gcaccaccag atggcaatcg
    13021 cccatcttca gcagtgcggg taaagcgtca tacgattgca gcgggaaaaa ttgcatgttg
    13081 cgcagtccac gctgctgcgc cattttttcc agccgcgctt tgccgccgcc ctgcccgaca
    13141 atggcaaaaa tcagcggttc atcgcgcagg cgatcggcag cttcaataac gttttccagt
    13201 ccctgctttt caccaatatt gccggagtaa agaatgattt ttttgttatc cggcaggcca
    13261 agctggttac gaagggcatc aacatcggcg tctgcaacat gctgaaaacg ggcgatttcc
    13321 gaccagttgg ggaagaaaat gatgttatcc gccgccacgc ctttttcgat ggctttattc
    13381 atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat gcagtccgct acgttcgaac
    13441 gccgttgcca gctgtgccac tttgccgcct ttgccttttc cggcaaggcc cagccccagc
    13501 atggcgtcca cttcgtaatc ctgaatatgc agcacggtac gcgcaccaga gagtttcgcc
    13561 agcaggcgca ttcccggcgt gcaaaacagc gttggcacca cgccgataat gcgatccggc
    13621 ttccagcgac gttgcgccat cagtggaaag aaactgctga cggcaaaact gccgagatgc
    13681 aacaggcgtt tcagggtgct cggctgtttt ggcacgtaca gcgggcagcg ccacaccgtg
    13741 gcggccccct cttctcgttt gtaccgccag gcggaatagt tctcgcccac ctgccactgc
    13801 gggtagtaag gcggtgcggt aataacccgc acctcatgac cttgtgccgc cagccattcc
    13861 accatctcgc cagtgtattt gccgatgccg gttaactccg gcgagtagtt aatgccgtag
    13921 accagtattt tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc
    13981 tgttagcgtg aacatcgtta ctggcgagca gcgcgtccgg cgtcagccag cggtaatcgt
    14041 catgctgctc atccggcagt aacagctctt cttccgctac tctgaagcga aaaccgagca
    14101 ccacatagtg agtggtgaaa tccgtgccgg agaagttatc gtcataaaag tgctgccaga
    14161 caccgtaaaa ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc
    14221 gctcaaatgc ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt
    14281 aaccctgcgc cgggcggttg gttcttttgc caagcagaaa ctcgccgcga ctgttctcga
    14341 caataaagtc gagagagaca agcggagtgg agcgcactac cgtggcaaag tcttcctgac
    14401 gtaaaaacat cattaccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct
    14461 ggcaagcccc gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac
    14521 atccagcagt ttgcgcggcg taccatccgg cttgctggca tcaaaaacca cccgaccttt
    14581 gtaatccacc actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt
    14641 accaacgtta atgtgcgaca gcatcggctg ggtgttctcc agccagactt catgcgccag
    14701 ctccatgaca tgaatgctcg ccgccgccat atcatcgacg tgcagaaatt cacgcatcgg
    14761 tgtaccgctg ccccacacca ccacatccgg tgcattctgt gccgtcgcct cgtggaagcg
    14821 acgcagcaat gctgggatca catgcgagtt actcggatgg aagttgtcgt gtggcccgta
    14881 caggttggtt ggcatgaccg agcggtaatc gcgtccgtac tggcggttgt aagattcgca
    14941 caatttgatc ccggcgattt tggcaatggc ataaggctcg ttggtcggct ccagcgtacc
    15001 ctgcaataac tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tacaggacga
    15061 tccgagaaac agcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc
    15121 aatcatcatg ttctggtaga tgaaatccgc cggatacgtg ttgttggcaa caatgccgcc
    15181 cactttcgcc gccgccagat agacctggtc aatgctttcg ctggcaaaga aatcatgcac
    15241 cgcgcggctg tccagcaggt tcagttcatc gcgggtgcgt aataccagct ccacatcacc
    15301 gcgctgttcg agctgtcgct tgatggcgga accgaccatc ccgcgatgac cagcaataaa
    15361 aactcgttgt ttactcatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat
    15421 ttcagcagag agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc
    15481 tctctgaggg tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgccg
    15541 agcagcgttt caacttcagc cggacggaag taacgcgggt caacagcgat aatcacatca
    15601 cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca
    15661 acacccgtgc cttcaaagcg cagtttgatg cccaactgtg ctgccgccat ttccacgaac
    15721 tgacgtacgg agtactgaac gccggtcgcg ataacaaaat cttccggttg ttcctgttgc
    15781 agcatcatcc actgcatttt tacgtagtct ttggcatggc cccagtcacg cagggagtcc
    15841 atattgccga ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggta
    15901 attttgcggg taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaataccg
    15961 ttacaggcgt acatgccgta ggattcgcgg tagttaacgg tgatccagta ggcgtacagt
    16021 ttggcgaccg catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc
    16081 tgcaccagac cgtacagttc agaggtggaa gcctgataga aacgagtttt cttttccaga
    16141 ccgaggaagc ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta
    16201 tattctggtg actcaaaaga gaccgcaacg tggctcattg cgcccaggtt gtacacttca
    16261 tccggctgta cttcacgcaa aatgcgcgtc aggttagagg tatcactcag gtcgccataa
    16321 tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg
    16381 gtgttgaatg acgatgcgcg acgcttaata ccatgcacct cgtaaccttt ttccagcaga
    16441 aactctgcca ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac
    16501 atgtcttatt cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac
    16561 tgcgggattc ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc
    16621 acccacgacg gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag
    16681 ccagcatttc tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg
    16741 gtcgtggcta ccggtgcata aataactttt ttgcgatatc accgaatgtg cgccaatggt
    16801 tatttcaccg agggtatata aattgacgtc atcgccgacc cacgcgtaat cacctaaggt
    16861 taatttccac ggataggtaa tttttactga cggacgaata actacgtttt ttcctatttt
    16921 tgctccgaat aaacgtaata aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc
    16981 aaatattgtt gcctgtactg cccaccataa ttgcacttta atagcgttgc cgccccggaa
    17041 ccctttcggc accgagaatc cacttaaatc ctgcatcgtt ttcccttata ttcagacttt
    17101 gttatataag gctttcgtct ttgaggtagt acgttggcgt aaatgccagg ataattcagc
    17161 ccaaaagcca ggtacatgta atatttgtcg ttggactttt ttcgcgtcag cacacaattc
    17221 catattattg gtggtagata ccccacccat ggaaaattca gacaccaggc cattgagttt
    17281 tttaaatgca taaccggctt tatacatttt ggctgccagc gcgtagtcgg aagaaacttt
    17341 atattccagg tcataacgcc atttcttcaa gccggatacc gggaaaaata tcgcctgatg
    17401 actggcgggc aggctgtgat aaatatacca gcccggtttg gcgctacgtt taattttatg
    17461 cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc acgttatctt tttgcatttt
    17521 taacttacgg acaaaatttg cggcattctg atgaaaaata tcgcccgaat tgagaaacaa
    17581 cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg gcgtcataga taccgttatc
    17641 tggctcgctg acaaagcgta ggttatagat accattgaga ttttccagat actcacgggt
    17701 gccgtcattg gaaccgccat cgacaacaat ccattcgaag ctgatatcgt ccgcctgcgc
    17761 cagatgcgcc agcgaggcat gtgttttgac tatcccttcg aggttacgaa acgcgacagt
    17821 gattatgcta agcaacatgt tcttattctt acctcgtaat atttaacgct tttcgcaaaa
    17881 taaacggaca gacgattaaa aatgcatatt ccgggctaaa tatcgaaccg gtaaaaaaca
    17941 gcgataccgg agtaaaaaga tataattgca cgcgaaaatt gcggttatca ccaaaagcgt
    18001 tgatcatcat ttttatcact ttccccatgt accacagcga taaaaacacc gcgaaccagg
    18061 aaaaataaat aatcagcaga tacaaaccat tgtctatggt ttttccgaca tccgcaccgt
    18121 taaatattcc gaatgatgcg acatattcat aaagtgagcc aaatctgact acaccgtcaa
    18181 tatgggtcaa agaataaccg accatcacca gcggaccgac aatacgataa tacgatgacg
    18241 atccttccgt acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac
    18301 caaccaggaa tacagccaga gaaatcaatg ctaacggtaa ctttttctta atcgcctctt
    18361 tattcagata ttggaatgcc cactccagca aataaaacag gataaaggtc ataacccctg
    18421 aaaacgatcc ggataatatt atccctgcga gaatcatagc atcggtttta ggcgttttga
    18481 taccaaactg tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt
    18541 cgaaataaag tgctgtcgtg cgcttgccgc cgaatttgat gaaattcagc acatagctgt
    18601 tgctgtaaat cagatatttc gaaatcgact ccataatact gctgccgccg gtgaggataa
    18661 tttgcgccat ctccaccgcc gccagcgcca ccaccagccc gaccaccaga taaaagaaac
    18721 gtaatatctt gcgatggttg tgtggcgaaa tcgttttaaa gcgaatactc cacaccatgc
    18781 caataatgat cacaatatag acaaacagca tggttgaagt gacgtattta ctggcatcca
    18841 acgactgacc aaacagatag ttgaacgccg tgagtcccgc accaatccct aaggcaatca
    18901 tcaatttttt aacgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga
    18961 cgatagtgat ggggaaactt tcaccaagct gggcgatttt gacgttaacc agcaggtaga
    19021 tcagcggcag cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc
    19081 tgttgtccac tgtaggcggc gcggctgcgt tggctgaact cagccagcgt ggtaccaaat
    19141 atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt gcagcacctc ttcttcactg
    19201 acggttttac caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcgatc
    19261 accggcacgc caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacgcgagaa
    19321 ctgaacacca gcgcatccat ctgattgagc gcgctcatca gcttacgttt gtcggtttca
    19381 aagccgtgat taaccacgtt accagcggtg aacggcgaga acttaccaaa ggtatgcagt
    19441 tcgattttgt cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg
    19501 taacgcaggt catgcgccac caccgcgatt ttcggcttac cctgggtttc gcgcaccgga
    19561 ggcagatccg ccagaatcgc ttcggttgcc atatcaatac cgttattgat aatccggcaa
    19621 cgccctggac cgtacaggct attgaaagcg tcagccacat gctggctggg ggaaataaac
    19681 tgacagccca gcgccagcat ctcacggaat aactggcgtt tgcccgccac cagttggtgt
    19741 gcgcgatcaa tcttcaccgg cggataatta tttaaggtcg ggcatttctg gcagcccgtt
    19801 ttccagcctt cacaaccgtc ggtaaaggcg cagcgtccgg taacgctcca gtggtcgtgc
    19861 agcgtccaga ccagagtgac gtccggttta tggtttttca ccttttcgca aaagcgcacc
    19921 acgctcttaa gatttagcca gtagctgtgc agcacatgaa aatgcaggac caccggaccc
    19981 ggtgtacgag taatggtgcg atataactca ttgaaattgc caaacagatc gcgattaaac
    20041 agacgaaaca ggacaatatt cgccattgca gtcatccgcg gcgtatgttt gatgacctgc
    20101 ggatagtttt gatggctgac gctctctttg ccgcctttgc cgtaaccgta gacaaaatgt
    20161 gacgctagcc cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg
    20221 ccttccgcca gtcgcacatt aaattgcaaa atattcattt aattaccttc actcgcgctt
    20281 tttctcccac caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca
    20341 cggtgacgtt gttaccaagc gtgatatcac caagaataat aacgttggca ccgagttcga
    20401 cgccgttgcc aatatgtgga catgccatgt tatcggcacc acgattgccg atagtgacgc
    20461 cgtggcgaat ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac
    20521 cgtgatggat agtaaagcgg cggccaatgg tcgcggcagc ctggatttca taaccgaaaa
    20581 agcattcggt gataatgcga tacagcacca gcagaggggc cgcccacaga ttgttgagga
    20641 cgttcttttt gcgccacacc gaacaaaaat gagcgacgcg ataggctaga accatgcagc
    20701 acggacgtaa actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat
    20761 accatcagcc agacgtttgc cgttacgcac cgacagcagc gtcagcaaag tgcgccaggt
    20821 catacgttta ttgcggatct ggtagagggt aaacagctga tattttttgc tggcgcggtc
    20881 gaatttgtct ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga
    20941 ggtgatctgc atttcaccgt gattgatatg caggatctgc gtcgcctctt ccactttcca
    21001 cggttcgcca tactccacca ccatccgcag gaagatgtcg taatcctgtg ctgctttcag
    21061 ttcggtatcg aacaagcact ctttgaaacg ccacgcccag gtaaaaacct gattgccaat
    21121 gatattgcgt ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct
    21181 tgctggttgg gaataaactt ctccctggca tacgtagtcg ttagcgtaca aaaaggcgtg
    21241 agtgaccagt tgctgtttat gggcgaggaa gacgctcagg cggttgggtg tccattcgtc
    21301 atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc ataatagcct ggttacgtac
    21361 cgcgcacgcc ccgctgttaa tatcgttgtg gatgtaagtg atacgcggat cgttgagggc
    21421 ggtgacgtac tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc
    21481 ccagttgctg tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg
    21541 gttccaggtt ggcatataga ttgagatcag cggattatct ttcataggtt gttctcccgg
    21601 cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg
    21661 tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa
    21721 tcctgatacg cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata
    21781 ccgttttgct caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa
    21841 cgcgccacca ttaacgtggt tccaacatga cgaccaacaa ttgccgcatc ggtcactgcc
    21901 agaatcggcg gcgtatcaat caacaccagg tcgtagtttt tactcgccca gttcaccagt
    21961 tcggcaaagc gttcgctcat caacagttca gaaggatttg gcggtacctg accgcgcggg
    22021 atcaggtcaa atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca
    22081 atcagaattt ccgacaggcc attgacgtta ttggtgccca acagctcgtg ggtgtagcct
    22141 ttgcgcatat cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc
    22201 aggttggcgc agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca
    22261 ttgttctgcg cctgcatcat cgcgaagtgc agactggtac gcagactgcg gatggcttca
    22321 atcgccagat cggttggatt ccccaccgcc agtagctggc tctgtttata gcgtttaatc
    22381 cctttgatgg ttttgacgct atcgcgcgct ttctgccatt ccgacaacgg gatgctggca
    22441 tagacgctga taccgtgttc ttccagcacc tgcgggcttt cgatgccgcg attaaacaac
    22501 gagcgcagca gcacacccac gatagagagc atcaggccaa gaataatcgc cccaaggata
    22561 atcagccctt tcttcggttt cagtacacct ggctgggtga ttgccgggtc aacaatgcgc
    22621 acatcgccga cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc
    22681 atatagacct gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctgggtt
    22741 ttcggcatcg ccgttacgcg accattaagt ttggcttttt cgtcttccag cgcctgacgt
    22801 ttctccagca gcgtgcggta cgcggggtga actttggtgt acagcttgga gatttccgcc
    22861 tctttaaagg tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct
    22921 ttcgcttcca gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt
    22981 tcggcaacat cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt
    23041 ttcgacgctt ccgccgattt gcgctcaata ttttgttcct gatagttacg ggcgatgctg
    23101 ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct
    23161 gcgtctttgc cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac
    23221 gtggagtatt tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc
    23281 agcgtgacgc cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca
    23341 tcactgctca gagtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctggtccgcc
    23401 atctctttcg ggcggttaaa ggtggtcact ttcaccgtct cgttctgacg tcccatcagg
    23461 cgatcccaac ccgcaccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtct
    23521 agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc
    23581 gatgcaggcg gtttgttggc taatgccgag ccaatgtcct gcactaacga attgccgctg
    23641 ttttgttcga tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa
    23701 accacagcac agagggcaaa tacagcggtg atgccaatca cccaccagcg cgcttcaatg
    23761 acggtgccga ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt
    23821 tgttttactt tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc
    23881 gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg
    23941 gatcggggat ttcacattcg ttatcccagt gaccaaacag catcactttg ccacgcatct
    24001 caggtgccat ctcgcataag cgttcgatat ggcgcttttc catggtcaaa atcaggtcat
    24061 agttgcgaca caggcggcgg ctgatttgcc gtgcacagtg accttccaga gacagttgat
    24121 gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc
    24181 cagcggactc cactttcagc tccgggtgat aacgttgcag taagcgttcc gccgtcgggg
    24241 aacggcaaat attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt
    24301 atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg
    24361 attgatcaga cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg
    24421 ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta
    24481 gatattggca atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat
    24541 gccgctggcg ttggaggtgg tcatgtcgat accttcggca ttgcccaggg cttcagtcag
    24601 ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa
    24661 cactttcaga tcatcattac gcggcacgta gagaatatcg ccggggtaaa gcagtcggtt
    24721 ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa atgcgttctt cacgaccatt
    24781 gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat
    24841 ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg
    24901 acctgagata taggcctttt gcgagcggaa ggcggcgata ttaacatcca cctgcgggtc
    24961 agcgatgtac gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc
    25021 gactacgtgg accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt
    25081 gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg gtgagttccg ggtgatccca
    25141 gacggtgaca ttcagaacgt cgcccggccc gacgcgatac tggtaattcg cgatctcgct
    25201 ttccagcgtc atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg
    25261 cggggtcagc ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt
    25321 gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat
    25381 caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt
    25441 atcactttgg cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt
    25501 accttgcagt taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat
    25561 ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc
    25621 ccctggcttt ttagctacca atacactgat ttagcttaat ttttcaaacc ctctccgcat
    25681 acaatcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta
    25741 tggtaattga atattggctc tccaataatg caggaggaag tgttacagct aacggaatag
    25801 caggcaaggt aacaattcgg caattggcta tttttaagaa ttatattaag tgtcattcaa
    25861 tatggttttt aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat
    25921 atttcttaga taatgcttta ttattaccac ttttaattca gggataatgt gaggttatta
    25981 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
    26041 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
    26101 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
    26161 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga
    26221 ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctggcgtc
    26281 aatctcctgg ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag
    26341 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
    26401 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
    26461 gttctgggga gtggtgacac aaattgtggt actggacgcc atcttctcac tcgactcagt
    26521 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
    26581 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
    26641 gatcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
    26701 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat
    26761 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
    26821 gctgcgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc
    26881 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
    26941 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtcagcag
    27001 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagacgagat
    27061 ccgccagtta ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgatgc
    27121 agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga
    27181 accgctcaac ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt
    27241 gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga
    27301 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa
    27361 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
    27421 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
    27481 gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcaacg
    27541 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
    27601 ggaaagccat cgcgtgcaga aggtgcagat tattccactg cgtaaggatg gcgagatgga
    27661 gtacgaggtg tgatggtatc ggtcatttta cccgatagcc gaaaggaata agatgaggga
    27721 acgcgccagc ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc
    27781 accctggcac gttcaccggc ttgagcgttc catagtagtg aggaacatca agtgaacgga
    27841 acagccttcg atcttacata ttcagaatat aatggtgccg ccgcgaacgc ctgatgcgac
    27901 gctttacgcg tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag
    27961 caacttcttc acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag
    28021 gcgacgtttc gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcagactgta
    28081 attgagttgc tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc
    28141 aatcagtttg ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca
    28201 ggtttgatct gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc
    28261 ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc
    28321 aagacgcgtt acgttatcga agttttcagc ggccttcaca tcaccaccat tacgttctac
    28381 cgcttgctga atcatctgct ggaagttcat cccttccatg cgcgtgtcgg tcatttcgac
    28441 atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa
    28501 atcaccagcc agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat
    28561 ggtaccaatc tcaacgtttt ccagccgtgg ctggaagttt atccgcggat ttattgatgt
    28621 cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta agcttgccct gcagttgagt
    28681 aatttccagc aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc
    28741 gcgccagcgc acgttactgg cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg
    28801 atatgccggt tcatcaatac gcgaagaaat gaccgggcgc ggcaatgttg attggctctg
    28861 cccctgctgc gcggcaccgt tttcaccatt cgctgtttca ttaagcggga tgaggttgtc
    28921 aagattcagt tgcgggaatt gcagtctcag ctgccattca ggtttctctg tcagcgtgac
    28981 ctgtgcttgc ccgctcagcg tgctgtcatt ggcggtcaag ctgatttggt taaatgaaag
    29041 gcgtttatgt gactcctgcc actgggcctg gaaactcccc tgcccctgaa taccttgttt
    29101 tggtaaatct gcaccctgca actgccagtt aatttgttca atagccgccg ttaaatcatg
    29161 cggataatca gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg
    29221 attaacccgc ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag
    29281 gcggatattg cggattgtca cctgctcgtc atcttcatgc tggaacacca gcacgctgtc
    29341 cgccacctta agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa
    29401 ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt
    29461 cagttggatc actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag
    29521 tggtaaaagc gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc
    29581 ctgggcggtg agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg
    29641 cagtggcccg tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata
    29701 gtcgcggaaa tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac
    29761 gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa
    29821 ttagtcttta tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc
    29881 acggcggttg taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat
    29941 taacatgccc ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat
    30001 gcaaccagac cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg
    30061 cgccagtgag gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc
    30121 cagcgtcacc gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag
    30181 aacgatctca tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct
    30241 cagatcgata aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac
    30301 atccaccgtc gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc
    30361 ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc
    30421 gcatcactcc cttaacgcca attacgtcaa gggcatacca ggttatcact gaacggtaca
    30481 caatttatca agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga
    30541 tgcggttttt cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga
    30601 attgcaggaa catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac
    30661 gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat
    30721 caacgaaaat ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc
    30781 cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt
    30841 caacatagct gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct
    30901 caagcaacag gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt
    30961 ccatcgacag atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt
    31021 catcaccgac ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg
    31081 aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt
    31141 tagcgacctg attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt
    31201 atagggattt catccgcctg ataccagtcg aatagcgctg ccgcgcgctc agagttaatt
    31261 gttgacgaag aattcccggt ggcaaattac gttgatcagt tttatacaag gtaaaaaatg
    31321 ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa
    31381 aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc tggtcttgcg
    31441 gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg
    31501 cttcacctgg tcagtttagg tttagtctcg tttatcttta cccttttctc gcttgagctt
    31561 tcgcagtttg gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg
    31621 ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgctc gctgggaaat
    31681 atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc
    31741 aatattgttg aagccgtgct cggggcagtg ctgctacgta aattgctgcc gtggtataac
    31801 cccttgcaaa atctggctga ctggctgcgt ctggcattcg gcagcgccat tgttccgcct
    31861 ctgctagggg gtgttctggt tgtcctgctg acgcccggag acgatcctct cagggcattt
    31921 ttgatatggg tactgtcaga gtccatcggc gctctggcac tggtgccgct gggattgtta
    31981 tttaaaccac actatctgct gcgccatcgc aacccacggt tgctttttga gtcgctgctc
    32041 acgttagcca tcacactgac gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt
    32101 actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt
    32161 ttgatcttcc ttaccacggt gatgatggtg tcgctgatga tggccgcgga tccctccctg
    32221 cttgctacgc cgcgtacata cctgatgagc catatgccgt ggctaccgtt tttgctgatc
    32281 ctgctgcccg ccaacatcat gaccatggtg atgtatgcct ttcgtgcgga acgcaaacac
    32341 atttccgaaa gcgaaacccg ttttcggaac gccatggaat attccgctat cggtatggcg
    32401 ttagtgggca ccgagggaca atggctgcaa accaacaaag cgctctgcca gtttctcggg
    32461 tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc ggaggatctc
    32521 aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac ctattcaatg
    32581 gaaaaacgct actacaaccg caatggcgat gttgtctggg cgttgcttgc cgtctcactg
    32641 gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga cattaacgag
    32701 ctaaaacgca ccgaacaggt gaatcagcaa ctgatggagc gcatcacgct ggctaacgaa
    32761 gcgggcggga ttggcatctg ggagtgggag ttgaagccga atatttttag ctgggataag
    32821 cggatgttcg agctgtatga aattcctcca catatcaaac cgaactggca ggtgtggtac
    32881 gagtgcgtgc tgccggaaga tcgccagcat gccgaaaaag tgattcgtga ttcgttgcaa
    32941 tcacgctcgc cctttaaact ggaatttcgc attaccgtaa aagacggtat tcgccatatc
    33001 cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag tcgaacgcct gctcggcatt
    33061 aatatggata tgactgaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc
    33121 ctgcacataa cgctggactc catcggcgaa gccgtggtct gtattgatat ggcaatgaaa
    33181 attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcatta
    33241 ggtgttccgc tcctgacggt gttgcatatt acctttggcg acaatggtcc cctgatggag
    33301 aacatttaca gtgccgacac ctcacgttcc gcgattgaac aagatgtggt gttgcactgc
    33361 cggagcggcg gcagctacga cgtgcattac agtattacgc cgttaagtac tctggacggc
    33421 agcaatattg gttcggttct ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc
    33481 cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaatcg cgccagtttt
    33541 gagaagcaac tgcgtatgct gctgcaaacg gtaaacagta cacatcagcg acatgccctg
    33601 gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc
    33661 gacgctttgc tgcgcgaact ggcgtcgttg atgctgagta tgctgcgttc cagcgacgtg
    33721 ctggcgcgac tcggtgggga tgaatttggt ctgctgctac cagactgtaa tgttgaaagc
    33781 gcgcgtttta tcgctacacg cattatcagt gccgtgaatg actatcactt tatatgggaa
    33841 ggccgtgtgc atcgggtagg tgccagtgcc gggattacct tgattgatga caacaatcat
    33901 caggcggcag aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtgga
    33961 cggggccggg tgacggttta cgaaccacag caagctgccg cacatagcga acgggcggcg
    34021 atgtcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat gatggcccac
    34081 ggtgtcgctt cgccgcggat cccggaagcg cgtaatttgt ggctgatttc gcttaaactc
    34141 tggagttgcg aaggcgagat tattgatgaa caaaaatttc gtcgtagctt cagcgatccg
    34201 gcacttagcc atgctcttga ccgacgggta ttccacgaat ttttccagct ggccgcaaaa
    34261 gcggttgcca gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc
    34321 gccacgctgg tgaatgatct gcttgagcag ctggaaaata gccctctacc accacggtta
    34381 ttacatctga ttattccggc agacgcgatt ttagatcacg cagaaagcgt gcaaaaactg
    34441 cggctggcgg gatgtcggat agtgctcagc caggtgggcc gcgatctgca aatcttcaac
    34501 tcgctgaaag cgaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg
    34561 cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc
    34621 gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttctggc
    34681 atcggcgtcg atctgattta tggtgatgtg attgccgatg cccaaccgct ggatttgctg
    34741 gtgaatagca gttatttcgc gattaactga gggagcgttt gcccattgcc tgatgcgacg
    34801 catcaggcct acaattcgtc cgttccgtct ggttgccagc cttccgtata ccagatatgc
    34861 aacagcgcat aagaacgcca gggcttccag cgctcggcat aacggcggat ttgtgccggt
    34921 gtcatccccg gaaatcgctg tttaatcaga taatcatccg gcagaaaaac atctttcgcc
    34981 tgccagccac gcaaagcgaa ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc
    35041 agcgttttca tcgcctgctc cacatcgccc ggaattgtca ttggtaagct gccctccagc
    35101 gccgcatttg ccagatgaat cagtgcctct gcccgtttca acggcatgcc taacgctttt
    35161 aatgcctgcg ggtcggctgc tgccagccgc tgaggcgtgg ggaagcagat atactccgga
    35221 aaatcatcca gccgttcgcc ataaagctgt acaactttgg cagtcaattt tgccgccatc
    35281 gccacgctca ccagttggcc taaaatcgcc cgcacaccct gctcaaaagc atcaatacag
    35341 ccgggtaaac gcaatccggg ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc
    35401 tgcgggttac attgcagatc aaacaggcgg ctcattttcg ccagacactc agcggcaacg
    35461 ggttctaaac ctgcacttaa atttatgtgc agagtatggc gggctatatc cggaatagca
    35521 gtcaccacgc cgcgatattc gcccaccgcc agactacggg cataataatc ctcagcgacc
    35581 gtttccacac cactcaccgc acgggcggcg agaaatccca acatccacga ccagtcatac
    35641 ggcggctgcc agttcagggt atacatcgca tctccttatt catccgcttt cagcataaac
    35701 gttattcaga cgctacgctt tgctttcata ttccggttgt cgcgacggca acatttcgct
    35761 aaagtcacgc cccttcttca ccggcatggg gattaattcg tgtttattgg ttttgattac
    35821 ggtacagcaa actgttcagt ggcggtcatg cgtgacggta aaccgcaatt actgaaaatg
    35881 gaaaacgaca gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga agcagtaagt
    35941 gaatggctgt accgccatca tgatgttccg gcagacgacg atgaaacgca ggcgctgtta
    36001 cgtcgggcga ttcgttataa ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag
    36061 ttcggtcttt cctcactggc acagtacatt gatgatccag aagaagtgtg gtttgtgaaa
    36121 tcaccaaaat cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag
    36181 gatctggtct gcgcaatgat gttgcacatt cgccagcagg cgcaggcgca gctgccagaa
    36241 gcgattactc aggcggtgat tggtcgcccg atcaacttcc aggggctggg cggcgatgaa
    36301 gcaaacgccc aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg attcagggac
    36361 gtggtattcc agtacgagcc ggtcgcggct gggctggatt acgaagccac cttgcaggaa
    36421 gaaaaacggg tgctggtggt ggatatcggc ggtggtacga ctgactgttc attgctgctg
    36481 atggggccgc agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt
    36541 tgccgtattg gcggtaacga tctggacatc gcactggcgt ttaaaaacct gatgccgtta
    36601 ctgggcatgg gtggcgaaac cgaaaaaggc atcgccctgc cgattctgcc gtggtggaat
    36661 gcggttgcca tcaacgacgt acctgcgcag agtgatttct acagtagtgc caacggtcgt
    36721 ctgcttaacg atctggtacg cgatgcccgc gaaccggaaa aagtggccct gctacagaaa
    36781 gtctggcgtc agcgtttaag ctatcgcctg gtgcgtagcg cagaagagtg caaaattgct
    36841 ctttcaagcg tagcggaaac ccgcgcctca ctgccgttta tcagcgatga actggctacg
    36901 ctgattagcc agcaagggct ggaaagcgcc ctcagccagc cactggcgcg gattctggaa
    36961 caggtgcaac tggccctgga taacgcccag gaaaaaccgg acgttatcta cctgaccggc
    37021 ggtagcgccc gttcgccgct gattaaaaaa gcgctggcag aacagttgcc gggaattccg
    37081 attgcaggcg gcgatgactt tggctccgtc accgccgggc tggcacgctg ggcggaagtg
    37141 gtgtttcgtt aaaagaaaat ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt
    37201 ttttctgccg cctgacgtaa aggttccatc aacggcatgg tccgtgctgg agcatgaagg
    37261 cggaatcgct gcaactctgt cgcgccgaca gtcaacgtcg attccagccg atgtcgtttg
    37321 gcggtcattt tcgccattac cgcttgtctg tctgctggcg ttatcgcttc attcggcctg
    37381 aacacaaaac ggcgttcaca gctcgctttc cagtcgataa ccgcctgggt cagatggtcg
    37441 ccaaaccctt tcacttgctt aacgccacga cgggtaacat ctgcggctgt ttcaatacca
    37501 aaagaccgta acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc aacatcaata
    37561 aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cagtgtcatg aagtgctgcc
    37621 agagcgcgtt tctcttcttc cggtaacccg agaatttcgt ccttcatttt ttcgagcatc
    37681 gcccgtttgg cgataaaacc ttccagcccg cccaactgtt ggatctggct gactaaatgc
    37741 tcatagtcca ttttcgcgcg attaaatgcc tcacggcgtt gctggatttc tgctttgtac
    37801 gctttgcttg tcagactgcc aataatccag atagccgaca gcacaggaac caaaataata
    37861 taacgcggtt ctgcctgaag gccgcaaagc aacagcgata acgctgacag tgcgatctca
    37921 atcagaatga tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg
    37981 aaatgatcgg gtaatggcaa ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc
    38041 cagactttcg ccagcacaaa atcaccgccg gtggtaatga cctcttcgcc gagatcaata
    38101 aaatagataa cgccttgatt atccagcgca caccacgggc agtcggtcaa atgagcggga
    38161 taaacatgca ttgccgaaac gggacatttc tttaactgtt ggcgcagaga atcaagtgcc
    38221 gctacccacg ctttagccgt cggacgcccg gttgccacgc cactttccgt gaatgactgc
    38281 tgaaacatgg cttcaacatc gcccggtaac atcgatagcg gaatcgatcg tggcggcggt
    38341 tttaaaccac gtcgctgatt atctgacgcg taggcataac ggaaatgggt aatatccgtc
    38401 tccagcgcat tacccgcatc agagataagc ggcacaccag aataaggatg ccggccacca
    38461 aacaagacgt gaaaaatcag caacgcaagg ccaaaattat cgtgattttc tgtgcgttca
    38521 aagccgacaa atgacgacaa ggtttgtagc tctggcggcg taaaatgcga cacgccgact
    38581 tcgcataaat gcagtgttcc attggcgtta atctgaaagg agtcgctatc aatcagtacc
    38641 actttgctgt cgcgacctac cataaaactg ttctggttta cgtcacccac gacgtgcccg
    38701 tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg caacatagag tagaaaatcc
    38761 cacgcacaat gagggtaacg ttgacgacga tgtgccgggc tatagatcat atgaatcggt
    38821 tctttaccag aaacttttgg catcataaaa ccgataactt ttccgcctcg tccaccgtga
    38881 agcgttgcct gcggccaggc gacataattc aacaattgcg cgtcagctgt cgcagccata
    38941 aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta
    39001 tcgacaaact cattgatatc ataaaccgcg ccttcaccgc ctttgcccag ttcacggcct
    39061 ggcgtaacgc actcaccagt agcagtatat aaagtgggtt tcatggtaac cacagcgcca
    39121 gtgccaacgt tttatcatca tccgtacgct cgtttaccgc cgggctggac aaaaactgct
    39181 ttaacaattc aggtaataaa tcgagttgtt cctgcgttgc tgacgccagt ccattgaaaa
    39241 acggggtaaa gaaaggtaca tgaggagaat tatccagcat atttaacgcc agtcgctgga
    39301 taccatccgt aaatgctgca actttatgca cacgctcagt gctggtgaaa gtttccagtc
    39361 tggaaacggc atcttcatcg gtaatgaagt gcgtcatatt ggcatattca cccaccatcg
    39421 gcgtaagcgg caattgcaga ccgtgaccaa aatccaccac cacgccgcca tcgccaatct
    39481 gcataatcaa ggtgccatta gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc
    39541 gaactgctaa ttctttggct tccgcctcgg caaaaagtcg ctgacgaacg gtcagcacaa
    39601 tatccgtggc gaggatatca ttaagcccca attccccacc ctgcactttt tgcgacatat
    39661 aagccatcgc ttcattgacg gcgagcgtcg cgccttctcc tccctgcgaa acgctacctg
    39721 cgccatcagc aaggaacatc accagtaatg gctgctggtc atttagccag gcaacctgca
    39781 tctggcaggc atcctggcaa ggcaagtccg cgctgatgtg cgatgttcca acagcggatg
    39841 cataaaccag acgccagctc acactgacgt ccagcctttt ggcgcttcca gcacaacttc
    39901 cgttcccggc gtggagcggg aaaccgaacg cagcgaactg gataaccagc taaacagttc
    39961 acggaattgt aatccttgca gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt
    40021 catatccgcc ccctgaacac cgatggaaaa gaaagcaaac cttttatctt cttccccccg
    40081 aaacactttg ttggctgcag cctgccactc atcggttggt gcaccatcgg taatcaggaa
    40141 aatccacggg cgataatacg aaataccatt tgcacgatac tcacgtttcc gctcctctac
    40201 catatccagg gctttggtaa tggccgcacc cattggtgta tcgccctggg caaacaggat
    40261 gggcggaaaa aaattagcgg cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt
    40321 cacaatgccg agttccactc tttttaaagc cagcggatcg gcaagcagtt catcacgaaa
    40381 ggtcaccaat ccggcgttaa gttcattaat aggtctgccg ctcatcgagc cagaaacatc
    40441 cagcagcaaa atgcaggggc aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa
    40501 cgtgatttgt tcgctcataa gaaatccttt cttagagaat tatcgaggtc cggtatcata
    40561 ccgactgcaa taatgaaata agagcaaacg aagaaacttt cagaattagg cccggcggcg
    40621 cagaaaattt tgctattcgc ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg
    40681 agtgacataa tttcaggagt gagggttagg gagaggtttc cccctccccc tggtgttctt
    40741 agtaagcctg gaagctaatc actaagagta tcaccagtat gatgacgtgc ttcatcataa
    40801 ccctttcctt attaaaagcc ctcttctccg ggagaggctt tcccgtttca gcgtcccgct
    40861 gaaatcatcg gcttacctcc tttcgccctg aatgcagtct atcgctaacg cgtgaataaa
    40921 atagtttcct ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc
    40981 agactacaaa gcctgcggat tgacaatctt atcgtgaagg catactttca ggagtgaggg
    41041 tagagcgggg tttcccccgc cctggtagtc ttagtaagcg gggaagctta tgactaagag
    41101 caccacgatg atgagtagct tcatcatgac cctttcctta tttaaggccc cttcctcggg
    41161 aggggctttc ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt tcgccatgca
    41221 cttagtctat cgctgacttg cagataaaat agtttccagt gtgattattg cgtaacgtct
    41281 cgcaaagaat ttttgctggc aggatcgcag acggcaaagc ctgcggattg acaatcttat
    41341 cgtgaaggca tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    41401 agtatgctgg gtagctaatc actaagagca ccacgatgat gagtagcttc atcatgaccc
    41461 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcat ccggctgaaa
    41521 tctgcggctt acctcctttc gccctgtatg tagtctatcg ctaacgcgta gataaaatag
    41581 tttcctgtgt tattactgga tgcgtgctcg caaatgtgcc cgccattcag acgattcccg
    41641 atagtgtttc ataattcctc catttttctc ccttattggc tggctacact agtatcattc
    41701 cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg
    41761 gtaatcgtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    41821 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    41881 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    41941 caggcagttc cgcgttacct caccgggctt ggcaccatta ccgccgctaa tactgttacg
    42001 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    42061 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    42121 gcccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    42181 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    42241 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    42301 cagctgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    42361 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    42421 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    42481 caggcgcaaa aagccggaaa accgctggtg gtagaagcct gggatcgcac caattcgaag
    42541 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    42601 attaaagtga aagcacgcct taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    42661 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    42721 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa agtcagcaaa
    42781 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    42841 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    42901 gtagtggaag cccagagcgc cactactccg gaagagaaag ccaccagccg cgaatacgcg
    42961 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    43021 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    43081 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    43141 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    43201 cgccgctaga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    43261 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    43321 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    43381 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    43441 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    43501 aaatctcgca gatttccggt gtcggactgg tgacgctttc cggcggtcag cgtccggctg
    43561 ttcgcgtcaa acttaacgcc caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    43621 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    43681 gtgcggtcac gctttccgct aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    43741 tcatcgccta ccagaacggc gcgccgattc gtctgggcga tgtcgcaacc gtagagcaag
    43801 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    43861 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcatccgg cagatgctgc
    43921 cacagctgac tgagagtctg ccgaaatcgg tgaaggtgac ggtactttct gaccgcacca
    43981 ccaatattcg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    44041 tagtcatgat tatctacctg tttttacgca atattccggc gaccatcatt cccggtgttg
    44101 ccgtgccgct gtcgttaatc ggcacttttg cggttatggt gtttctcgat ttttcaatca
    44161 ataacctgac gctgatggcg ttaactatcg ccaccgggtt cgtggtcgat gacgccatcg
    44221 tagtgatcga aaatatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    44281 tcaaaggcgc gggtgaaatc ggctttacca ttatctcgct gaccttctcg ctgattgcgg
    44341 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    44401 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    44461 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    44521 cggaaaaaat gttcgacagg ataatcgccg cctatggccg tggactggcg aaagtgctga
    44581 atcatccgtg gctaacctta agcgtggcgc tcagcacgct gctgcttagc gtgctgctgt
    44641 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    44701 tgcaggcacc gcaatccagc tcctttgcca atatggccca gcggcaacgc caggtcgcgg
    44761 acgtgatttt gcaggatccg gcggtgcaaa gcctgacctc atttgttggc gttgatggca
    44821 ctaacccgtc gctgaacagt gcgcgtttac aaatcaacct caaaccgttg gatgaacgtg
    44881 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    44941 tcgatctctt cctgcaacca acgcaggacc tgaccatcga tactcaggtc agccgcaccc
    45001 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    45061 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggaca
    45121 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    45181 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    45241 atactcaggc taaccagtat cgcgtggtgc tggagcacaa caccgaaaat actcctggcc
    45301 tcgcggcgct ggataccatt cgcctgacca gcagcgacgg cggcgtggtg ccgctaagct
    45361 caattgccaa agttgaacag cgttttgcgc cgctttccat taaccatctg gatcagttcc
    45421 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    45481 cgattatgga caccgagaag acgctgaatc tgccggtgga tatcaccacg cagttccagg
    45541 gcagcaccct cgccttccag tcggcgcttg gcagcactgt ctggctgatt gtcgcggcgg
    45601 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    45661 ttctctcgac gctacccacc gcaggggttg gcgcactgct ggcgttaatg attgccggta
    45721 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatc gtgaagaaga
    45781 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    45841 gcgatgccat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accactctgg
    45901 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    45961 gtccgttagg tatcggcatg gtcggcggcc tgattgtcag ccaggtgctg acgctgttta
    46021 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    46081 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgtccggtgg
    46141 cgacgatttt actgtcggtt gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    46201 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    46261 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc
    46321 gcattgccgg agtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt
    46381 tgcagtttga ttttgaccgg gatatcaacg gcgcggcgcg cgatgtgcag gcggcgatca
    46441 acgctgcaca aagtttgctg cccagcggga tgcccagccg tccgacctat cgcaaagcta
    46501 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    46561 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    46621 gtgatgtcga tgtcggcggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    46681 cgctgtttaa tcagggcgtc tcgctggacg acgtacgcac tgccatcagc aatgccaacg
    46741 tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    46801 atgagctaaa aaccgccgcc gaatatcagc cgctgattat tcattacaac aacggcggcg
    46861 cggttcgtct gggcgatgtg gcgacagtga ccgactctgt acaggatgta cgtaacgccg
    46921 ggatgaccaa tgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata
    46981 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    47041 cggcgattga tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag
    47101 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    47161 tgcgctcggg tcgcgcaact attattcccg ccgttgcggt gccggtttcg ctgattggta
    47221 cgtttgcggc gatgtacctg tgcggtttca gtctcaataa tctttcgtta atggcgctca
    47281 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc
    47341 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    47401 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    47461 gcggattgcc gggccgactg ttacgcgaat ttgccgtgac gctttctgtc gccattggta
    47521 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggttggatg ctgaaagcca
    47581 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    47641 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    47701 tgcttggcac cattgcgctg aatatctggc tatatatctc gatcccgaaa accttcttcc
    47761 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    47821 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcggtgg
    47881 ataatgtcac cggctttaca ggcggttcgc gagtgaacag tgggatgatg tttatcaccc
    47941 tcaagccacg cggcgaacgc agcgaaacag cgcagcaaat tatcgaccgt ctgcgggtga
    48001 agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    48061 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    48121 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    48181 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgtgacacca
    48241 tggcacggct gggaatagac gtacaagccg ccaacagtct gttaaataac gccttcggtc
    48301 agcggcaaat ctcgaccatt taccagccga tgaaccagta caaagtggtg atggaagtgg
    48361 atccgcgcta tacccaggac atcagcgcgc tggaaaaaat gttcgttatc aataacgaag
    48421 gaaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    48481 tgaatcatca gggattatcg gcggcctcga ccatttcgtt caacctgccg accggaaaat
    48541 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    48601 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    48661 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    48721 gttatgtgca cccgctgacg attctctcca ccctgccctc ggcgggtgtt ggagcgctgc
    48781 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    48841 taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac
    48901 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    48961 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    49021 gcgacggctc ggagctgcgg caacccctgg ggatcaccat tgtcggcgga ctggtaatga
    49081 gccagctcct tacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    49141 tgcgtttttc gcgtaaaccc aaacaaacgg taaccgagta aatgacagat cttcccgaca
    49201 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    49261 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    49321 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    49381 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    49441 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    49501 tacagggcgt tggcggcgcg atgatggtgc cggtcggcag attgacggtg atgaaaatcg
    49561 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    49621 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    49681 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    49741 actacaccat gcagacgcgg cgctttgatc tctccggatt tttattgctg gcggttggca
    49801 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctgacga
    49861 ttgcaggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    49921 ataacaaccg cgctcttttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    49981 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    50041 tcctgcaaat tggcctcggt ttctcaccgt ttcatgccgg actgatgatg atcccgatgg
    50101 tgcttggcag catgggaata aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    50161 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    50221 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gttattacaa ggaatggtca
    50281 actcaacgcg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    50341 cgagcagcgg caacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    50401 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    50461 ccacacaagc cgtctttatg tacacctggc ttagcatggc gttgatcatc gcccttccgg
    50521 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    50581 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    50641 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    50701 gcgtggcttt attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga
    50761 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    50821 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    50881 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    50941 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    51001 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    51061 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgctc tggcaacgtt
    51121 actcgcggcg ctcgccacct ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    51181 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    51241 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    51301 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    51361 agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat tcacaccgga
    51421 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca
    51481 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    51541 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga tttgccagcc gtggcctgaa
    51601 actgcagttt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    51661 gttattcaat aacttactcg aaaacagcct gcgctacact gatagcggcg gcagcctgaa
    51721 aatctctgcc gggcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    51781 tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg
    51841 caaccgtgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    51901 acataatggt cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    51961 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    52021 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    52081 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    52141 gtgcgccaga caccaccgga tctgatcctg ttagatctga tgctccctgg caccgatggc
    52201 ctgacgctgt gccgggaaat tcgtcgtttt tctgacattc cgatcgtgat ggtgacggca
    52261 aaaatcgaag agatcgatcg cctgctgggg ctggagattg gcgcagatga ttatatctgt
    52321 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    52381 ccgcagcgcg agttgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt
    52441 tttcaggctt catggcgcgg taaaatgctt gacctgacac ctgcggaatt tcgtctgttg
    52501 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    52561 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc
    52621 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    52681 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    52741 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gcaaactgac
    52801 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaacagca
    52861 cctttagatg ctgttttcga tacgcaattt caatcaagga gtcattatgg ctggttggtt
    52921 tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    52981 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    53041 ggtgcgtagc aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gtaacggcaa
    53101 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    53161 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac
    53221 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttatat
    53281 ccaaataacg cttagttatt aataattaag agtaaccgcc gccattaaaa tagtgggcac
    53341 tgacgagcac tcgttgtgct ttctttgttt accatcccat acttatcctc ctgaacatgt
    53401 tcatgtgcca aaatatcttc taatactttc gtgacgctat ccggtttatg acatacactg
    53461 atgtaagatt tttacttaca ggcgggcgta aaatggccag aacgatgacg gttgatctcg
    53521 gcgatgagtt gcgtgaattt atcgagtcct taattgaatc cggcgactat cgcacccaaa
    53581 gcgaagtgat ccgtgagtca ctgcgattac ttcgagaaaa gcaagcagag tctcgcctgc
    53641 aagcgttacg tgacatgctc gcagaagggt tgagtagtgg tgaagctcag ccgtgggaaa
    53701 aggacgcgtt cttacggaag gtgaaagcag ggataaggaa ataatgtgcg tattattaaa
    53761 ctcatgccaa aagccaatga ggatttagaa ggcatctggt attacagcta tcaccatttc
    53821 ggcgaaccac aagccgacag atatgtggaa catctttcag acgttctcca gattctgagt
    53881 aacaataata taggtacgcc cagacctgaa ttaggcgaag gtatttttgt tcttcctttt
    53941 gaacgccacg tcatttattt cctgcaatca ccaggggaaa ttatcgtcat tcgtatcctt
    54001 aaccaaaacc aggacgccac cagacattta cactggagct aaacccgccg attgcgctac
    54061 aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggatctg
    54121 acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg
    54181 ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg
    54241 cgttattccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaatcttca gctcggcatc
    54301 aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac
    54361 gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg
    54421 ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc tgaaatgccg
    54481 atccaccttt cggtgcaggc taacgccgtg aactgggcga cggtgaaatt ctggcagcaa
    54541 atgggcctga cccgcgtgat cctctctcgc gagctgtcgc tggaagagat tgaagagatc
    54601 cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggcgcgct gtgcatggcc
    54661 tactccggtc gctgcctgct ctctggctat atcaacaagc gcgacccgaa ccagggcacc
    54721 tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgatgttggc
    54781 aacatcgtac acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggtatc
    54841 ggcgcaccaa ccgacaaagt gtttatgatc gaagaggccc agcgtccggg cgagtatatg
    54901 accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgccatc
    54961 gcccatgtag aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggtcgtacc
    55021 aaatctttct actattgtgc acgcaccgca caggtttacc gcaaagctat cgatgacgcc
    55081 gctgcgggaa aaccgttcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt
    55141 ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac
    55201 ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggg
    55261 gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg
    55321 acgccgcaag gcaacattaa ttttaccctt gagcacatgg aaaacgccaa aggcgaagct
    55381 atgccgatag caccaggcga tggttatact gtgtggctcc cggtcccgca ggatcttgag
    55441 ctcaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa tccccacggt
    55501 aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca
    55561 gctgtattta ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc
    55621 tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt attttttatg
    55681 cataagccct atccctggtc accgtcttcc attgaccaca tcgatagaat ctcccttcat
    55741 agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta agcacaacca
    55801 gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctccacattt gaatgtcaga
    55861 cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg catttttttg
    55921 ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc accagatatt
    55981 ctgcccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga
    56041 atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt atgcacagca
    56101 caaccagaca taataaaaat actaattaat gatatagcag ctatcttttt catctcacct
    56161 tcccccatta aataccaacg acactctcta gtgtttaaat ataataatgg catgattatt
    56221 ataattgaat aggattataa taaatgttct gtacaacatt tcctacataa gtaggaatta
    56281 cggacattga ggcccttcag ggtaactcca tgggggcttt aatatatttg gaatagtata
    56341 tttaagatgc cactgtttgg ttgaatatta ggcatatgct cttttttgaa atttatcggt
    56401 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    56461 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    56521 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    56581 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    56641 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggcg
    56701 atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    56761 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    56821 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    56881 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc attgcgatag
    56941 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    57001 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    57061 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    57121 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    57181 gtggcggtca gcaattgtgt ccgaacgcgt taattaacga tggcttgctg caactgcgca
    57241 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    57301 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    57361 cttttaatct tgatggcgaa ccgttgagcg gacaaaactt tcatattgaa atacttccgg
    57421 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    57481 tcttgaacca gggagaatcc gaaatgccgg atgcagtgta aataccttat ccggcataca
    57541 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag tttatccacc
    57601 tcagacacag catcatcggt aataataatg tcaatttttt cgattggcag tacctgatta
    57661 aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt agcggcggtg
    57721 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    57781 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
    57841 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccgcagac gaccagagta
    57901 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    57961 tttttggcgg tcattaatcc ttcggcgata agcatcgtcg tacttccgct gtcgagaata
    58021 acggtcatcc cttcgtggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    58081 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    58141 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    58201 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    58261 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    58321 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    58381 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    58441 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    58501 aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt cagcaaccgg
    58561 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    58621 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    58681 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    58741 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaaacgcag ttcgcgtaaa
    58801 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    58861 gattttgcca gtgccagttt ttctgagcta atgtcgatag ccgtcacact ctttgctccc
    58921 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    58981 tttttattct cacaaccttg cgctaaatga aaagcatgca gaccaacggt aatcggctca
    59041 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    59101 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    59161 cactgggaat aaaacccttt cagacactct ggacaagtaa aacagggtaa taacggcaca
    59221 caggcaaccg catcgtcagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    59281 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    59341 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    59401 ttaatttcag gaatgacgct ttctgcaacg cgcacgatac catcagtatc attcaccact
    59461 gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt aaaaattatt
    59521 ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag gtcatgaaaa
    59581 taccggtcag ataaattgca ccgataataa tgaaaccggg aatattttgc ggggagaaaa
    59641 cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc
    59701 cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg
    59761 cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg aacagatttc
    59821 cacgatgcac ggcgaccgcc atcgccacga agaagccgat ggtggcaaga tcgccaaacg
    59881 gcagcacctg attacccggc acacaaacag caattaaaat ggtgagtggg ataaaaatca
    59941 ggcttgccga taccaccgcc gtatgtccca gcagcaacgc cggatcaagg ccaatcagga
    60001 actcctgacc gccgaacttc gcctgtaaac gactacgagc ctgcttagcg atgggcgtta
    60061 aaccatccat gatgggttta atcacccgtg gcattagcag catcactgcc gccgttttta
    60121 ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca
    60181 tcacaaaacc cacggtgaca ggctcgccaa atggaccaaa tttgcgctga atatcgtcgg
    60241 cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg accagcaccg
    60301 caatcggccc catatacgcc gacgtaccgt gcggaatagc aataccttcc agctcaaaga
    60361 aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta
    60421 ccacacctgc catccctatc atccatgaac cggttgccag atgcagcaac gcgccggtga
    60481 aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata cgggtcagta
    60541 acatcgccac gttaaccaat atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc
    60601 aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc aggtcgaaat
    60661 tttccgccat cgctttcgcc gccggaccaa tggaatccag cattaagcca atcacaaggc
    60721 caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg cctgccttca
    60781 tgcctaatat tttagaaaaa ataatgatga caatcggaag catcaccgta gggccgaggt
    60841 cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt
    60901 tattttgtaa tgcttcgata ccgacaccag aaataaaagg catgccgtga actaacggaa
    60961 tatcgccaaa actacgatcc actttggcag tggtgcatat caaatgcaca ccatccatat
    61021 aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta tgattctgac
    61081 acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga
    61141 caataatctt gcgtttcata aatagtcctt tttattgtta cagagagatt tattcgtctg
    61201 aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga gtgattagtg
    61261 tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct
    61321 gctgcggatt ttccacaatc aacgcaataa ccaacgatac ctccacgtcg ttatcatcat
    61381 ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag
    61441 catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag
    61501 ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc acacctttgg
    61561 cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc
    61621 tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc
    61681 gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg
    61741 actgaatgcg ttcaaattgt ttggaaagat actgactaat catgcccagt gggatgtcca
    61801 cgccttgcag attcaccatc atcgtttcga cgctattttt aatccgacta tgcggccagt
    61861 aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg
    61921 tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca
    61981 ggcaaccgct gcgattttcg ggggcgataa gttcctgctc aatttgtgcc agtgcaaata
    62041 tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc
    62101 ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca
    62161 ttcgggtatt ttctatccat tgcgccagcg cctgcgcttc ctgcggctga taatggataa
    62221 tattgctgtg atcaaattcc acacccggct gcaccacgat ggcaatcacg cgtgttaacg
    62281 cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat
    62341 cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa
    62401 cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg
    62461 cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat
    62521 cccccgcgca ggacattgac gcatcaagat gaattttact gaagccggca cgaacatatt
    62581 cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc tgccagcagt
    62641 ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca actttatcgg
    62701 caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt
    62761 gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg
    62821 ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt
    62881 caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa atggcaggtg
    62941 cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt
    63001 gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg ctcggtcagg
    63061 taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat
    63121 atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc
    63181 accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaattc aagcgccggt
    63241 gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt
    63301 gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc ttcattgact
    63361 tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct gacatcaaag
    63421 cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc
    63481 gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat
    63541 ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc catcgcgctg
    63601 accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg
    63661 atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt ttcgagattg
    63721 tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag catttgcttt
    63781 gtcgatacca cgtacatttt catatcctgt cgtttgtttt cgatttcaaa atataatgaa
    63841 attatttgtt tttaaatatc gagataacga tcacaaaaac gacaatatga aaattgttcg
    63901 aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc cgtagcaaag tgcgcgtgtt
    63961 tttatgccgg atgcggtgta aacatacgtg gcagtgctat gtaggcatga taagacgctt
    64021 ccgcgtcgcg tcaggcatag gttgccggat gcagtgtaaa cgccttatcc gtcctacggg
    64081 gtggtgctgt tcgtataagg cgtatcaggc aatagtaatt ttgctatcga gataaacgtc
    64141 ctgcacggcg ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc
    64201 aagaatcagc cccattccgc cagcgcgttt gttgataacc gcagtacgca ctgcatcact
    64261 gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc aacccagccc ggcccatata
    64321 gcagttagct aactgataac gcaccagatc aatcgggttt tcgctggtta acttgctgta
    64381 cacgcgatcg tcggtataac cgtaattaat cgctttatag ccgccgttat tttccgccat
    64441 tttttgtttg acgatatctg cgccgatggt tgccgccaga tggtttgcct gaccggtcag
    64501 gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac gcaaataggc
    64561 ccacagcact gtcaccatac ccagctcgtg cgcacgttca aaagccgcag aaatttcttc
    64621 aatctggcga cgtgactctt ccgagccaaa atagatagtc gcaccaaccg caaccgcgcc
    64681 catgttgaac gcctgctcca cgctggcata cagcgtttga tcgtaggtat tcgggtaact
    64741 tagcgtctcg ttgtgattaa gtttgacgag aaatggaatg cgatgcgcat agcgccgcga
    64801 taccgacgcc aacacgccgt aagttgatgc cacacagtta cagcccgctt cgatcgccag
    64861 ttcaacaatg tttttcgggt caaagtagag cgggttagca gcaaatgaag ctccggcaga
    64921 gtgctcaacg ccctggtcaa ccggcagaat agaaagatat cctgtgccag ccagacgccc
    64981 ggtgttgtac aacgtctgca tattacgtaa caccgctggc gggcgattat tgtcaatcat
    65041 cacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa tagtcataca
    65101 acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac
    65161 tatgctcccg taaattccga ttggatatcg gctatggatt ttcctggccc gccttttgcg
    65221 ggcaatcata atcctggtcg ttacgggcga gcttttccat cttttggatg ttttttcagc
    65281 gttttctgtt ggctcgattc atcagaaaaa atgttagcgc ggtcaagttt tcaccgcaaa
    65341 ggtatttaaa aggtattatt aagtggtatt gtcatcgcgt accttacatt acctgtcatg
    65401 aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg ttgaatggtt
    65461 tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg gttttagtgc
    65521 cggagaaatt ggctggtcgt atgcctgtac cgccattgcg gcgatcctgt cgccaattct
    65581 ggttggctcc atcactgacc gctttttctc ggcgcaaaaa gtgctggcgg tattgatgtt
    65641 cgcaggcgcg ctgctgatgt atttcgctgc gcaacagacc acttttgccg ggttctttcc
    65701 gttactgctg gcttactctc taacctatat gccgaccatt gcgctgacta acagcatcgc
    65761 ttttgccaac gtgccggatg tggagcgtga tttcccgcgc attcgtgtga tgggcactat
    65821 cggctggatt gcctccggtc tggcatgtgg tttcttgccg caaatactgg ggtatgccga
    65881 tatctcaccg actaacatcc cgctgctgat taccgccgga agttctgctc tgctcggtgt
    65941 gtttgcgttt ttcctgcccg acacgccacc aaaaagcacc ggcaaaatgg atattaaagt
    66001 catgctcggc ctggatgcgc tgatcctgct gcgcgataaa aacttcctcg tctttttctt
    66061 ctgttcattc ctgtttgcga tgccactggc gttctattac atctttgcca acggttatct
    66121 gaccgaagtc ggcatgaaaa atgccaccgg ctggatgacg ctcggccagt tctctgaaat
    66181 attctttatg ctggcattac cgtttttcac taaacgcttt ggtatcaaaa aggtattatt
    66241 gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta gtgcggatga
    66301 atatttcacc tacgcgttac tgttcctcgg tattttgctt cacggcgtaa gttacgattt
    66361 ttactacgtt accgcttaca tctatgtcga taaaaaagcc cccgtgcata tgcgtaccgc
    66421 cgcgcagggg ctgatcacgc tctgctgcca gggcttcggc agtttgctcg gctatcgtct
    66481 tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa ccggtaaacg gactgacttt
    66541 caactggtcc gggatgtgga cttttggcgc ggtgatgatt gccattatcg ccgtgctgtt
    66601 catgattttt ttccgcgaat ccgacaacga aattacggct atcaaggtcg atgatcgcga
    66661 tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg tgctctttat
    66721 gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccacg cagccgcgtt
    66781 aaagcacact ttggctggat tgaccgtttt cttcctggac caaaggagaa taacgcggcc
    66841 tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg tctggcggat
    66901 gcgttactgg aacgtgaagg caagatcgat ccggatctga ttgggcgtaa tattctcgac
    66961 tgggcgctgc gtttcgacgc ctttaacaaa aacgtactag gtccgacctc gaagattgcg
    67021 cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaacaacgg cgtgaccaac
    67081 ggcgcagcga tgcgcgtctc gccattaggt tgtttgcttc cggcgcgtga tgttgattcc
    67141 tttattgatg atgtagcgct ggcgtccagc ccgacccata aatccgatct ggcggttgca
    67201 ggcgcggtag tcatcgcatg ggcgatttct cgtgccattg acggagaaag ctggtcagcg
    67261 attgttgatt cactgccttc aattgcgcga catgcccagc aaaaacgcac caccacgttc
    67321 agcgcctcac tggcggcgcg gctggagatt gcgctgaaaa ttgtgcgcaa tgccgacggc
    67381 accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag cactattgag
    67441 tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa tcgctgcgcc
    67501 gtcctgtgcg ctaaccttgg cggcgacaca gacaccatcg gtgctatggc gacggcaatt
    67561 tgcggcgcgt tgcatggcgt taacgctatc gatcctgcgt taaaggcgga actggatgcg
    67621 gtaaatcagc ttgatttcaa ccgctatgcc acagcgttgg cgaaatatcg tcaacaacgg
    67681 gaggtggtat gagcggcgct cgattacaca cgctgctgcc tgaattaacc acgcgtcagc
    67741 cggtgatggt cgtcggcgcg gcggtcattg acgtgatcgc cgacgcttat gccctcccct
    67801 ggcgtgggtg cgatatcgaa ctgaaacagc agagcgttaa cgttggcggc tgcgcactga
    67861 atattgccgt ggcgttaaag cgcctcggca tcgaagcggg taatgccttg ccgctcggtc
    67921 agggcgtgtg ggcggagatt attcgcaacc ggatggcaaa agagggctta atcagcctga
    67981 tcgataacgc cgaaggtgat aacggctggt gtctggcgct ggttgagccg gatggcgaac
    68041 gcacttttat gtcattcagc ggtgtggaaa atcagtggaa tcgccagtgg ctggcgcgat
    68101 taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc tcgccctgcg
    68161 gcgaattgtt agtggaatgg ctggaagagc tgcaagacgt gacgccgttt atcgattttg
    68221 gcccacgtat tggcgatatc ccggatgcat tactggcgcg gatcatggcc tgtcgacctt
    68281 tagtgtcgct caatcgtcaa gaggctgaga ttgccgccga acgttttgct ttatccgcag
    68341 agataacaac acttggcaag caatggcagg agaaatttgc cgcgccgttg atcgttcgtc
    68401 tcgataaaga gggtgcatgg tatttcagca acgacgcttc tggctgcatt ccggcatttc
    68461 caacgcaagt tgtagacacc attggggcgg gcgacagtca tgcaggtggc gtacttgccg
    68521 ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca gtggcgtcgt
    68581 gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta ctcctcgcac
    68641 acaaaaacgt atagatcgct gcgacagtgg ctaatgctgt actcaatagg ccgttgctgt
    68701 tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa ctgaatatgt
    68761 gactgaaact cggcatccgg catccgggcg ctaacgcggg aacgtgtacg ttgtgggtaa
    68821 atgtgctggc tgcggaaata gtcataaagt gaaatcccga tggcatcaac atcatgaatt
    68881 aaatgcgccg gaacccacga ttcctcaatc gataccgctt cttcatcgac atagcgaata
    68941 cgcttaagca aaaagacatc acttcctgct tcgaccgcca gctgctgcgc gacttcttcg
    69001 ggacatttca cgacacgctt gttaacccat agcgtatcgg gctttttccc gcgcaatacc
    69061 acctgctgag aaaaacctcg cgcttctttc aacgagtatt cgaagatgtt gttgatctgc
    69121 gtgccgtaac cgcgcgaacg cgtcaccaca ccctcttctt ccagcgcctg catcgccttg
    69181 cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc gggcaaaata
    69241 ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc aaacttaata
    69301 taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat cagctgggta
    69361 tgcgcttgtt ccatttgtct ttttcctgcc agggagtctg gtgggagtat atcactgctt
    69421 cttatttaag gcgtttcttt aatcccatca acgaatccct gcaactcctc caccgtgcca
    69481 ttaaacacat taaaatccac cggaccattg atgccatcta cctgtccacg gtcggaatgc
    69541 tgccagaagc gccaggccat tccgtcattg tccggacgac gttgataata gtgagccacc
    69601 caccacggat attcattaaa atagcccgcc agattcgtgt gataaaaaac ggctcctgag
    69661 taaataatcg gcttttttcc cgtgcgtttt tctaccattt ttagccactg acttacccgc
    69721 ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagcac ggcagggaaa
    69781 tcgccttgtg agaaatccac cgtttgcaga aataatcgtg cctgaaccgg cgctgctacc
    69841 gacggggaga aataatgata cgccccgcgc agcaggccat tttcgcggct tagttgccag
    69901 ttacgcgaaa aatacgggtc caccagcttt tcgccttccg tcgccttaat aaaagcaaac
    69961 tgtaagcgga taccgttgtc gcgcattttt gccacccgct gccagtcgat ccgctcctgc
    70021 cagcgagaaa catctattcc gtgaatggtg taactggcag gaatgcgaat agcgaaagat
    70081 ttaaccgggc gataaccgta gaaatggatg tagtctgtaa tctgaactgc tgtcgaatag
    70141 aaaaaattca ccgtctgacg cggataaatt gcaacgatgg aaattagccc aagagcgcat
    70201 aatagagagg tgagtttttt tcgactggta attcttagct gcattggttt catccctgaa
    70261 tgtcagtgcc aaaggctgac aataaccaaa gcagctatag tacggtgctc accaccaggc
    70321 gtggtagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg cctgggctaa
    70381 tgctgatgaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg tatggcgcgg
    70441 gcgtagtgca gccaacgccg cagagagcgt acaaccagtg ccgtgggtgt ttttggtcat
    70501 aatgcgcggt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt ccgggctttg
    70561 ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg ccaacagcga
    70621 tcgcccttgt tccagcattt cctgttcggt gcgcgcgtgt ggcgcgtcga gcaaggcggc
    70681 agcttcgggc aagtttggcg ttattaatga aacctgtggc aataatcgac tgcgcagcgt
    70741 agcaaccgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa cggtgtcgag
    70801 taccacgttt tggatctgat aacgttgcaa ccgttctgcc accgcttcaa caatatcggt
    70861 ttccgccaac ataccgattt tagtggtgtc gattcgcaca tcgctgaaca ccgaatcgag
    70921 ctgggcggcg acaaaatcag gctcaatgcg atacaccgac tgtacgccac gggtattttg
    70981 cgccaccagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga aggttttaag
    71041 atcggcctga atccccgcac caccactcgg atcagtaccg gcaatcgtca gagcgttaat
    71101 tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa atgtggaaca
    71161 aaactgcctg gcccctcgct tctggcgact gcgcgctctc cggcttgttt catccagtga
    71221 caggcagatg cgacattttc cagcatatcg cctggtaagg cacaacaggc agcaacaacc
    71281 gccgataatg cacagccagt tcctaccact ttggtcatta gcggatcgcc accgtgaatg
    71341 ccaacgatac gatgcccatc ggtaacataa tccacctcgc ctgtgaccac gacgattgcg
    71401 ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc gtcagtggta
    71461 tccactcccc gtccgccatt agcaacgcca gctaatgcca tgatttccga agcattacca
    71521 cgtatcgctg tcggtttaaa agataaaaat tcatgacaaa aatggcggcg ataatcgagc
    71581 gcaccaaccg ctactggatc aagcgtccag ggtgtttgag agctttttgc ttgctcaacg
    71641 gcagcacgca tcgcctgtgc gcgtggctgc gtcagtgtgc caacgttaat caacaaggca
    71701 ctggcgatag ccgcaaactg actggcctct tcggtttcga taaccatcgc tggcgatgca
    71761 ccgagcgcca gcaaggtatt ggcggtaaag gtttgcacca catcgttggt catgcagtgc
    71821 acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc tgaactcagc
    71881 aggtcgactt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc tggtggtccg
    71941 tgacttccct acgctggcat tatccagatc aggtaatacg ggtatttctc agccttcaca
    72001 aagaagggca ccccgagtca aataaaacaa aatcaaaagc ttgcgattaa cgtcccgtta
    72061 atccctgttc tggatcatgc cagcgggaag ccaaagactc aaccctaaaa aatcctctgt
    72121 gtcccagttt gtgtctcacg ttgtgaccca cagataaatg gcaggcacaa aaaaaaccgc
    72181 ccataaggcg gcttaagtaa attattcagt cgtgaagatt taaatgaaat ttattttctg
    72241 catacgcttt cttcatgcag tcgaagtgct tttgccaatt ccaattggaa tgcaaaggaa
    72301 agacgggaac ggctgatctt gataccgtca ataatgatgt atgtagggta aacagtgacc
    72361 atgcggccca agtattggaa gtgtttaggg tggtttgctt tatcgttatt cattgtgtgc
    72421 catccgtctg ggtggggggt aatcccggca acggatgatt gctctaaaat cgatcaaaat
    72481 caataactta acagatcacc aaatccaggg ggtgtacatt ggcaagcctc cctcagccta
    72541 acttatacac gaacagaaaa taggggcgga ttccttccag ttgggtggga aaatatccgg
    72601 gagtttggat attgttgcta ggcccctcga tctgaggccc cgggtgatga gagtattttc
    72661 gccaaatatc tgggggcact ccctaccgga tggcctgcat tctggtcaaa cattgtccaa
    72721 ccttccgctc ttacagtgcg ctcgctcaga tatcccttta ttctcaatta cttaataaca
    72781 acacaatacc attctgttgt tttaccctgt ggaccagtgg cccagccggg ccaccctgga
    72841 ggcggacgcc tcccctgtgc gcctgtggcg cattatcttg tgacaagtaa tacaaaccca
    72901 cagggttaag aatggtagcg ccacaggcgc atacagagag cgggcctaca gaggcccttt
    72961 gaggggcgaa ggctttcgcc ccgattggta atgagaacga ctatcgttta caaatgatat
    73021 tgattctcat ataaggataa aaaagctggg gattttttct tgagggtaaa ttgaaaattt
    73081 gccaggcaac accataacga aacaataatt aattgcccac aaatacagta acttaaaggc
    73141 gctgggggag tcaaatcacc ttacccgcct tgtggtgata gttcattaag taaccatctt
    73201 ttacccacat aaacagccca ttacctcccc tgctgggtca ttactctgcc attaaacgcg
    73261 attaaacccg atttaattca ctaaaaagac atatcttaaa attaatcatt tttgtagttt
    73321 acaagtgact gtttttgatc aagaattgat cacagttagg ttctggaggg tacagaatga
    73381 atttcgtaga gggctgtaaa tcagttcagg aagtgaaagc ggtagcgaca gagttactca
    73441 aacagggcaa ggggctgacg ctttaccgtg acgcctggtt gctgggttat gaattaatgc
    73501 tgagggtgtc ggatctgcgc tctatccgtt atgacgacat caaaggcgat caccttgtcc
    73561 tgtcccaaca gaaaacgggg gaatctgtcc gtgtgaggct caccgatacc gctaaacgca
    73621 tcatagcggc ccggagagag gcgaaccctg atcatgtata cctcttacag ttggacagcc
    73681 acaggagcaa gggaaagcct gtgagccgct ccaaattgca tgaggaaatt agctacgccg
    73741 gggaaaagct ggggctgaat ctttcgaccc acagcatgag gaagagtaaa ccaactatcg
    73801 gatatgacaa tggggaggat atagccgtaa tcagtaaggc attggggcac aaatcgttat
    73861 ccagtacgct ccattacatt ggggccacac aacgcaaagt agatgatttc agtaataaat
    73921 attcaataga agatctgtta tgaaaagcct ggatattagg aattataaaa gagaaaaggc
    73981 tcccactagg ggagcctttt ttgtatgggc ggttagttcg gcccgccctg catcagcatg
    74041 ttgaaagcct gctggttgtt ggtctcaact tcagagcgga ccagatccct aacctgccca
    74101 tcctgcactt tcacattgac gttcacgtta agcggctggt ttagccacgc tggcggcgtc
    74161 atcagtggat tacctgccat atcaacttta agctgtggcg ctgtaatcgt cggcgcagga
    74221 ggaacataac ccggattgga gtaagagctt acccaccctg ttttatctgt tggtttggca
    74281 aagtctggat tagtcggaag gtctgggctg tggatgaaat cccacagttt acccagcggg
    74341 ctatctttct tcagattgcc tccggcggtg gtgtcctgtc catacatggc gtcgttagcg
    74401 tccatgactt tatcccaggc ataatcccca cctaccgaac cgatcgcaat gccccagcca
    74461 acaggaccgc cagagagagc cgccgcgccg cctaccttgc caactttcgg accccggccc
    74521 ggtttgtttg gcttgtcttt accttcccca ccagacccgc cagttgtagc ggcttcaatg
    74581 ccaccaatag cggcgatccc tttcatgacc cccagcaatt tggttagcgg gttgagcatg
    74641 gtcaacaacc atttaaaggc cccgcccaac ttccagatag agccagcgaa cagagccacc
    74701 ccggcggcgt atgctgccat ttctccccag gatttaagct gctcaccagt gatccccatc
    74761 ttagcggcgt aatactcaat aatgcggaag gtcaggatca cagtgtcgtg caactctgta
    74821 aacgcatctg tggcaccctc aacgattttc ccggcgatat gcccgaacat ctcaaaggcc
    74881 ccaccagagc cgttaacagc ggcgctgata gcgtcgaaag cgctggttag tgagttacca
    74941 aagccagaag taaagaagat attcatagcg ttctgcatac tggtttgcag gcgctgccag
    75001 cttgcccggt tggacttcat agccttatcc agagcgccgc cgttacgagc tgcggctttc
    75061 atctgtttag ccacttccgg gaggatgtcc ttagagaaca gcttcccttg ttgcatcagg
    75121 tcaaacagat ccttttctgt caggtctgta cggcctttca tctggttaag agccttaacg
    75181 aacagcccca cagcgcccgg aagagcttca gacaattggc ctttgagttc ttcactcatt
    75241 accgtgcctt tagacatcat ctgattcaaa ctacgcaagg cgcgtgattg ggattcggag
    75301 tctgcaccag taacaaggcc ccattcactt gcaccctcaa agatggatcg catctggtct
    75361 ttagggagct tgtcaccaac agcggcggca aactgggtat agtctttact ggtcttgagt
    75421 aaatccaggc ccaaacggtt ggactgatca cggagataat caaactcttt agcggcctta
    75481 tctgtggaac cggtgatcgc tatcagacca gaaagcgcgg attcaaaatc ctgcccggtg
    75541 ttcatgatct tgccggagag cgccacagtt gcggcaatgg tgatcgcccc gcctgcaccc
    75601 tcgatcatgg aggtgtccag tttgttaact ttggcggtta ctggaattgt ggctcccctc
    75661 atgcctaccc ggccttgctg gcgcatggtt tgctgaaggc gtgacactct ggcgttatat
    75721 tcctgcaatg ccatagaccc ggcgtgatac tggcgggtaa gctgtccaaa ctcctggagg
    75781 tgtccagcac gttgggccgg ggtaacattg gcgttagcgg ctgaagtgct gaagcggata
    75841 gctttagcgc ggattgtctg gagtttacgc tccgctacca gttgggcttt tgccgctttg
    75901 gcttccgttg cggctttagc tttggcctct gccattgccg cttttgcggc ttcatcctgc
    75961 tgtttgcggc gggctttggt atagccgttt tcctcaaact tgaggcgtag gggcttcatc
    76021 ttggacgcta ctttgtccca ctctttcccc acactgcgga tcttgcctac cgtctttttg
    76081 tagctacttg aatctacagt aaaaattgta cggtttacgg ttttcgtgat gatcacgttt
    76141 ccacttgcca ttattcagcc ccttccagtt tggtagccat aagcggacgg cttgccttgt
    76201 taatgttcac acggcggaga ccttccacag atttagttag cagtgataca gagcgcccgt
    76261 tagcgctgaa cccctgatcc ttgtgacaca atgcaattcc acggcttttt acattccggg
    76321 ccgggcctga ttctggaccc tctgagaggg tttctttgta ctggtcgatc aatgcttcca
    76381 gttctgacag agcggcggct gatttagtca cttcggcctg tgtggctttc tgacgttcca
    76441 acgctttggc ctgttccagt tgggccaaaa gtgttttgta tgcttccatg aggtgatcct
    76501 tacttagcga gagggcaaaa gagaaaatag agatttatta gggcgtagag cgtccacagg
    76561 gaccaacgcc cgaaaaggag aaataaaagt ttcaatggag ttaggcccgg cgggccgggg
    76621 ggtggttatg cggctcgttt ggaggagatg atcactgtgc ggagttcctt ccgcccggcg
    76681 ctaataatct tgcgtactcg tgtcatgctg gttcccaact tgtaaaccgg gttggctaca
    76741 tagcgaggcg ctcggaggtt agccaaaact tgatcgtatg gaagcatctg atccacaaga
    76801 tagcgggccg gggccacacg ggtgagcgtc tgcaccgtct tgacgttgat ccccaacatc
    76861 tcaccaatca tctgatccgg gagattgtcc gctttcatct gctgaatcat cacggctttc
    76921 tcgggaaggg tcagcacggc cttttgatac ttcacagggt tgtattcccg tttcatcttc
    76981 agggcatacg ctaacgacac attgaaagct gaacagatcg ccttagcact atgacccatc
    77041 acacaggccc gcttgatttg gacgatttga tccgttgtca gcttcttttc atccatcagg
    77101 gggcgcggct tgctggttgc cagcttcaga gagagggcgg cgatcttctc ttcctcttcc
    77161 ggggttaatg ctttcacttc agttgtgata atgctcaaga gaatctccta tcagaggggt
    77221 tgacttcaca cgggggattt gacaaaatga ggataatggg gatttttatt tgaaatttaa
    77281 caaaatcaaa aagctgggtc taaatcccaa tacctggatg gctccttgat ttcccctctt
    77341 gggtatggcg tcaccgctgg caccacagag gccgtttccc ccggttgggg atcagcttgt
    77401 acaagcccct ctcccaccgt ttccaccttc tcactgtaaa ccgtcatcaa tgtgatcggc
    77461 gtggtaggtc ctggcgttgc tggcacaatc tccggcgctt caggcgcttc tacaggctcc
    77521 gccgtgtggt tgggttcctg catttcttcc cgctccgctg tggccttctg cggctgttcc
    77581 tgcggtgctt ccgcttccac tgtacccacc atttccgggg tgatcgggtt tacagtgtaa
    77641 tggcacgtct tgccgccgat ctgcccggtc ttgttgatca ggcccatctc caccatttcc
    77701 cgaagcaaat tcctaactga tcggtcagtc aggcccaaac ggcgggataa aagctcctgt
    77761 gagaaatagg cggtttgccc attggtctgg taaccctcga tcagtgcata ggtgtaaacc
    77821 atgttcatgg tgaatacctt gccgttgatc ttctcaatgt ctgccagttc atgacaaacg
    77881 gcggtaaatg gcttacttgc tgttgctggc attctgtttt ccttctgcgg aagcgactaa
    77941 aagaggatgg tttttcaggt aggcggctaa cgcctccttt acgatggtat gaggggcacc
    78001 accgcccgcc cttaccagtg cataaagctg atcggcttgt tctttcggaa aatcaatttt
    78061 caaaggttaa tctccttcag ttcgtcgatg tcatcggctg acagactcag gccgaaaaat
    78121 tcaccccacc atttcgccgg ggccgtgatc gccggatttc ccagcgtcac ccttctggac
    78181 ggctcaacct gctgtaacat gctgtaaatg tcgtcataca atccgggggc gctgtcgtca
    78241 tcccggatcg agttaatgat ctgtgaggcg tacccattca caaggcgact ttccgaaaga
    78301 tccgtaaact ggtgcaacat atttcccctc cccattgttg attaattagt gaacaattac
    78361 aggcaattcc ccggtgatac gctcaaaatg atccgccgca tttaacatga tgaaggccat
    78421 tacctcgtca tattccatcg ggacgccttt tgcctcaaag agggattgga acgccagcac
    78481 aatggacgct tgcagatctg taggcttgga agcgaacagc gcaacggttg ctttcatttc
    78541 aagcgctttt tctgcgtcca tctcggtgac ctttgcagcg atacgttgag actggcgcat
    78601 aacgccggag gcgctggatt tggtcaattc aagggcctgc gtaatcgctt ctacttctct
    78661 catacgtccg gcggctttca tccggctacg ggtttctgtc agggtcttac gggtcaggtc
    78721 acgctgatcg acacgttcaa ccattttttc aacatcgagg taaatactca ttgcaatctc
    78781 ctttcaggtt agttatagta ttatggggaa ttgcataccc gatcaacccc ctcatcacaa
    78841 tatctatttt acactatttg acaatgattc gcaacaaatt tcttgattta tcttgcgctc
    78901 agttgtttat tgtgctagag gcacagaaag cctctgtaac gctctcagtg aggcacaggg
    78961 cgtttatagg gtgtttccct gtctacgtat gagaaagcgc catagcggca cgtttaggtg
    79021 ggttcctgtg gggttgtggt tctgtactgt atacggcact gtgtaccggg ccggggaccc
    79081 caccccgccg ccatcgtgtc accttcgcat cgctacggtg cttcccaaag agcaggaaaa
    79141 gaaaaccgcg ttgcggtttc tgttcctgcg attgccccaa aagggaggcg agcctgtaag
    79201 gctctttcct aaagagtctg atctcttgat tgttcaatca ataattactc acactctctt
    79261 taaggtcttg acctggccct gggggttcta gtcttatcgg gtgtgggtag gtttatctat
    79321 agtcttattg tcggaagatt tttccgcccc gcccggaaaa tatttccgcc caagaggcga
    79381 tcaggtagga aaatatttcc gccctgcttc tctttcggga taggccggaa aatatttccg
    79441 cccggaagat tcttcctccc caggagtgag ccacacaggc gagactccac attgtgggaa
    79501 acaggccccc ggcctaaaac ctggtaagcc cgaagggcga gaaggtttta agatcatata
    79561 ataacaaatg aaataattaa ttgtgtaaac aattagttat tgaatgaagt tgttatataa
    79621 gatcaataag acggacccgg agggcgagcg tagcgaggtc ttatatatct attattgttt
    79681 agtgtgcctt tcagggctac ataccccccc ttcaattcca cccacccatt aataaaaatt
    79741 aatgcaggta
//
LOCUS       DAMZII010000003        73255 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZII010000003 DAMZII010000000
VERSION     DAMZII010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.003"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            8..394
                     /locus_tag="POA77_000145"
     CDS             8..394
                     /locus_tag="POA77_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDI6363646.1"
                     /translation="MALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQ
                     MIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSL
                     EDILLEITSLVDNALDLAEITEEYSH"
     gene            413..1111
                     /locus_tag="POA77_000146"
     CDS             413..1111
                     /locus_tag="POA77_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDI6363647.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1162..1821
                     /locus_tag="POA77_000147"
     CDS             1162..1821
                     /locus_tag="POA77_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDI6363648.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1839..2237
                     /locus_tag="POA77_000148"
     CDS             1839..2237
                     /locus_tag="POA77_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDI6363649.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2247..2885
                     /locus_tag="POA77_000149"
     CDS             2247..2885
                     /locus_tag="POA77_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDI6363650.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2888..4051
                     /locus_tag="POA77_000150"
     CDS             2888..4051
                     /locus_tag="POA77_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI6363651.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWGEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4135..5760
                     /locus_tag="POA77_000151"
     CDS             4135..5760
                     /locus_tag="POA77_000151"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDI6363652.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5877..6152)
                     /gene="yjfN"
                     /locus_tag="POA77_000152"
     CDS             complement(5877..6152)
                     /gene="yjfN"
                     /locus_tag="POA77_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDI6363653.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6301..6630)
                     /gene="bsmA"
                     /locus_tag="POA77_000153"
     CDS             complement(6301..6630)
                     /gene="bsmA"
                     /locus_tag="POA77_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDI6363654.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6812..7561
                     /gene="yjfP"
                     /locus_tag="POA77_000154"
     CDS             6812..7561
                     /gene="yjfP"
                     /locus_tag="POA77_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6363655.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7558..8313)
                     /gene="ulaR"
                     /locus_tag="POA77_000155"
     CDS             complement(7558..8313)
                     /gene="ulaR"
                     /locus_tag="POA77_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDI6363656.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8421..9485)
                     /gene="ulaG"
                     /locus_tag="POA77_000156"
     CDS             complement(8421..9485)
                     /gene="ulaG"
                     /locus_tag="POA77_000156"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDI6363657.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9840..11237
                     /gene="ulaA"
                     /locus_tag="POA77_000157"
     CDS             9840..11237
                     /gene="ulaA"
                     /locus_tag="POA77_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI6363658.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11253..11558
                     /gene="ulaB"
                     /locus_tag="POA77_000158"
     CDS             11253..11558
                     /gene="ulaB"
                     /locus_tag="POA77_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDI6363659.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11568..12032
                     /gene="ulaC"
                     /locus_tag="POA77_000159"
     CDS             11568..12032
                     /gene="ulaC"
                     /locus_tag="POA77_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDI6363660.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12046..12696
                     /gene="ulaD"
                     /locus_tag="POA77_000160"
     CDS             12046..12696
                     /gene="ulaD"
                     /locus_tag="POA77_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI6363661.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12706..13560
                     /gene="ulaE"
                     /locus_tag="POA77_000161"
     CDS             12706..13560
                     /gene="ulaE"
                     /locus_tag="POA77_000161"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDI6363662.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13560..14246
                     /gene="ulaF"
                     /locus_tag="POA77_000162"
     CDS             13560..14246
                     /gene="ulaF"
                     /locus_tag="POA77_000162"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDI6363663.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14375..14650)
                     /locus_tag="POA77_000163"
     CDS             complement(14375..14650)
                     /locus_tag="POA77_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6363664.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14977..15372
                     /gene="rpsF"
                     /locus_tag="POA77_000164"
     CDS             14977..15372
                     /gene="rpsF"
                     /locus_tag="POA77_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI6363665.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15379..15693
                     /gene="priB"
                     /locus_tag="POA77_000165"
     CDS             15379..15693
                     /gene="priB"
                     /locus_tag="POA77_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDI6363666.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15698..15925
                     /gene="rpsR"
                     /locus_tag="POA77_000166"
     CDS             15698..15925
                     /gene="rpsR"
                     /locus_tag="POA77_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI6363667.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15967..16416
                     /gene="rplI"
                     /locus_tag="POA77_000167"
     CDS             15967..16416
                     /gene="rplI"
                     /locus_tag="POA77_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI6363668.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16530..17501)
                     /locus_tag="POA77_000168"
     CDS             complement(16530..17501)
                     /locus_tag="POA77_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI6363669.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(17554..18855)
                     /locus_tag="POA77_000169"
     CDS             complement(17554..18855)
                     /locus_tag="POA77_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDI6363670.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(18898..19374)
                     /locus_tag="POA77_000170"
     CDS             complement(18898..19374)
                     /locus_tag="POA77_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDI6363671.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(19395..21476)
                     /locus_tag="POA77_000171"
     CDS             complement(19395..21476)
                     /locus_tag="POA77_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDI6363672.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(21473..23014)
                     /locus_tag="POA77_000172"
     CDS             complement(21473..23014)
                     /locus_tag="POA77_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6363673.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23024..23800)
                     /locus_tag="POA77_000173"
     CDS             complement(23024..23800)
                     /locus_tag="POA77_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDI6363674.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(23810..24652)
                     /locus_tag="POA77_000174"
     CDS             complement(23810..24652)
                     /locus_tag="POA77_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6363675.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASKMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(24662..25453)
                     /locus_tag="POA77_000175"
     CDS             complement(24662..25453)
                     /locus_tag="POA77_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDI6363676.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            25663..26301
                     /locus_tag="POA77_000176"
     CDS             25663..26301
                     /locus_tag="POA77_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDI6363677.1"
                     /translation="MYLDTSHAQNLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIREKERELDISGLSPVIAIEKIIDFTWDYYINNP
                     WFLKIVHSENQSKGVHYKKSKRLAEINHAHLQSMASLLEEGQRQKTFKKDIDPLQINI
                     NIAALGAYYLINQYTLGMVYHISMVSPEALEARRRVIKETILSWLLVNPARG"
     gene            complement(26302..26931)
                     /locus_tag="POA77_000177"
     CDS             complement(26302..26931)
                     /locus_tag="POA77_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HDI6363678.1"
                     /translation="MPGRFELKPTLVKIWHAPDNFRLMDPLPPMHRRGIIIAAIVLII
                     GILLPGDETRQMPVVTREAQLDMQSPPPGEAQLQAHLVTPQNDPDQVAPVAPEPIQEG
                     QPEEPPVAETQSFQPESGIDQQWRTYRVEPGKTLAQLFRDHNLPPTDVYAMAQVEGAD
                     KPLSNLQNGQIVKIRQNANGVVTGLTIDTGNNQQVLFTRQPDGNFIRAR"
     gene            27150..27770
                     /gene="fklB"
                     /locus_tag="POA77_000178"
     CDS             27150..27770
                     /gene="fklB"
                     /locus_tag="POA77_000178"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6363679.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28079..29491
                     /gene="cycA"
                     /locus_tag="POA77_000179"
                     /gene_synonym="dagA"
     CDS             28079..29491
                     /gene="cycA"
                     /locus_tag="POA77_000179"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDI6363680.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(29536..30198)
                     /gene="ytfE"
                     /locus_tag="POA77_000180"
     CDS             complement(29536..30198)
                     /gene="ytfE"
                     /locus_tag="POA77_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDI6363681.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(30306..31271)
                     /locus_tag="POA77_000181"
     CDS             complement(30306..31271)
                     /locus_tag="POA77_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6363682.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(31380..32240)
                     /gene="qorB"
                     /locus_tag="POA77_000182"
     CDS             complement(31380..32240)
                     /gene="qorB"
                     /locus_tag="POA77_000182"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI6363683.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            32329..32709
                     /locus_tag="POA77_000183"
     CDS             32329..32709
                     /locus_tag="POA77_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6363684.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(32838..34781)
                     /gene="cpdB"
                     /locus_tag="POA77_000184"
     CDS             complement(32838..34781)
                     /gene="cpdB"
                     /locus_tag="POA77_000184"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDI6363685.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            34971..35711
                     /gene="cysQ"
                     /locus_tag="POA77_000185"
     CDS             34971..35711
                     /gene="cysQ"
                     /locus_tag="POA77_000185"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDI6363686.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(35701..36258)
                     /locus_tag="POA77_000186"
     CDS             complement(35701..36258)
                     /locus_tag="POA77_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI6363687.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            36583..36789
                     /locus_tag="POA77_000187"
     CDS             36583..36789
                     /locus_tag="POA77_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDI6363688.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(36851..38194)
                     /locus_tag="POA77_000188"
     CDS             complement(36851..38194)
                     /locus_tag="POA77_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6363689.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(38517..39155)
                     /gene="msrA"
                     /locus_tag="POA77_000189"
     CDS             complement(38517..39155)
                     /gene="msrA"
                     /locus_tag="POA77_000189"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6363690.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            39361..41094
                     /gene="tamA"
                     /locus_tag="POA77_000190"
     CDS             39361..41094
                     /gene="tamA"
                     /locus_tag="POA77_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDI6363691.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            41091..44870
                     /gene="tamB"
                     /locus_tag="POA77_000191"
     CDS             41091..44870
                     /gene="tamB"
                     /locus_tag="POA77_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDI6363692.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWRQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            44873..45214
                     /locus_tag="POA77_000192"
     CDS             44873..45214
                     /locus_tag="POA77_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDI6363693.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            45426..45677
                     /gene="chpS"
                     /locus_tag="POA77_000193"
     CDS             45426..45677
                     /gene="chpS"
                     /locus_tag="POA77_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDI6363694.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            45671..46021
                     /gene="chpB"
                     /locus_tag="POA77_000194"
     CDS             45671..46021
                     /gene="chpB"
                     /locus_tag="POA77_000194"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDI6363695.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(46101..46631)
                     /gene="ppa"
                     /locus_tag="POA77_000195"
     CDS             complement(46101..46631)
                     /gene="ppa"
                     /locus_tag="POA77_000195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDI6363696.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            46941..47897
                     /gene="ytfQ"
                     /locus_tag="POA77_000196"
     CDS             46941..47897
                     /gene="ytfQ"
                     /locus_tag="POA77_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDI6363697.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            48207..49709
                     /locus_tag="POA77_000197"
     CDS             48207..49709
                     /locus_tag="POA77_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI6363698.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            49723..50745
                     /locus_tag="POA77_000198"
     CDS             49723..50745
                     /locus_tag="POA77_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6363699.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            50732..51727
                     /gene="yjfF"
                     /locus_tag="POA77_000199"
     CDS             50732..51727
                     /gene="yjfF"
                     /locus_tag="POA77_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDI6363700.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLAT
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(51760..52758)
                     /gene="fbp"
                     /locus_tag="POA77_000200"
     CDS             complement(51760..52758)
                     /gene="fbp"
                     /locus_tag="POA77_000200"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDI6363701.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            52934..54307
                     /gene="mpl"
                     /locus_tag="POA77_000201"
     CDS             52934..54307
                     /gene="mpl"
                     /locus_tag="POA77_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDI6363702.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(54458..55009)
                     /locus_tag="POA77_000202"
     CDS             complement(54458..55009)
                     /locus_tag="POA77_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDI6363703.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            55103..56455
                     /gene="pmbA"
                     /locus_tag="POA77_000203"
     CDS             55103..56455
                     /gene="pmbA"
                     /locus_tag="POA77_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDI6363704.1"
                     /translation="MALAMKVISQVEAQSKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            56638..57024
                     /gene="cybC"
                     /locus_tag="POA77_000204"
     CDS             56638..57024
                     /gene="cybC"
                     /locus_tag="POA77_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDI6363705.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(57069..57533)
                     /gene="nrdG"
                     /locus_tag="POA77_000205"
     CDS             complement(57069..57533)
                     /gene="nrdG"
                     /locus_tag="POA77_000205"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDI6363706.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(57691..59829)
                     /gene="nrdD"
                     /locus_tag="POA77_000206"
     CDS             complement(57691..59829)
                     /gene="nrdD"
                     /locus_tag="POA77_000206"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI6363707.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(60223..61878)
                     /gene="treC"
                     /locus_tag="POA77_000207"
     CDS             complement(60223..61878)
                     /gene="treC"
                     /locus_tag="POA77_000207"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI6363708.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(61928..63349)
                     /gene="treB"
                     /locus_tag="POA77_000208"
     CDS             complement(61928..63349)
                     /gene="treB"
                     /locus_tag="POA77_000208"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDI6363709.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(63468..64415)
                     /gene="treR"
                     /locus_tag="POA77_000209"
     CDS             complement(63468..64415)
                     /gene="treR"
                     /locus_tag="POA77_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDI6363710.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            64600..64653
                     /gene="mgtL"
                     /locus_tag="POA77_000210"
     CDS             64600..64653
                     /gene="mgtL"
                     /locus_tag="POA77_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDI6363711.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            64794..67490
                     /gene="mgtA"
                     /locus_tag="POA77_000211"
     CDS             64794..67490
                     /gene="mgtA"
                     /locus_tag="POA77_000211"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDI6363712.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(67696..68082)
                     /gene="ridA"
                     /locus_tag="POA77_000212"
     CDS             complement(67696..68082)
                     /gene="ridA"
                     /locus_tag="POA77_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDI6363713.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(68155..68616)
                     /gene="pyrI"
                     /locus_tag="POA77_000213"
     CDS             complement(68155..68616)
                     /gene="pyrI"
                     /locus_tag="POA77_000213"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDI6363714.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(68629..69564)
                     /gene="pyrB"
                     /locus_tag="POA77_000214"
     CDS             complement(68629..69564)
                     /gene="pyrB"
                     /locus_tag="POA77_000214"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDI6363715.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(69568..69702)
                     /gene="pyrL"
                     /locus_tag="POA77_000215"
     CDS             complement(69568..69702)
                     /gene="pyrL"
                     /locus_tag="POA77_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDI6363716.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(69983..70378)
                     /locus_tag="POA77_000216"
     CDS             complement(69983..70378)
                     /locus_tag="POA77_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6363717.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(70509..71222)
                     /gene="bdcA"
                     /locus_tag="POA77_000217"
     CDS             complement(70509..71222)
                     /gene="bdcA"
                     /locus_tag="POA77_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6363718.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRWITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGTMHTIDGAFGA"
     gene            71293..71886
                     /locus_tag="POA77_000218"
     CDS             71293..71886
                     /locus_tag="POA77_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6363719.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            72031..72483
                     /gene="tabA"
                     /locus_tag="POA77_000219"
     CDS             72031..72483
                     /gene="tabA"
                     /locus_tag="POA77_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDI6363720.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            72606..>73255
                     /locus_tag="POA77_000220"
     CDS             72606..>73255
                     /locus_tag="POA77_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6363721.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILFPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDL"
BASE COUNT        17662 a        18238 c        19348 g        18007 t
ORIGIN      
        1 gaatccgttg gcgctggcga cggcgctgca aactgtacct gaacaaaata ttgatgtgac
       61 aaatagcgag agcgcattaa ttattaaaat gaatgattat ggcgatttgc aaatcaatat
      121 tctttttact tcccgccaaa tgattatcga aacctttatt tgtccggtaa gtagtatcag
      181 caatccagat gaatttaata ccttcttatt aagaaatcag aagatgatgc cgttgtcatc
      241 ggtaggaatc tccagcgtgc aacaggaaga gtattacatt gttttcgggg cgttatcgct
      301 taaatcttct cttgaagata tcctgcttga gataacctcg ctggtagata acgcattgga
      361 tctggctgaa attacagaag aatattcaca ctaattacaa ggacgggtat ttatgggaat
      421 tttaaaaagt ttatttacgc tggggaaatc gtttatctcc caggcggaag aatccattga
      481 agaaacccag ggcgtgcgca tgctggaaca gcatattcgt gacgctaaag ctgaactcga
      541 taaagccgga aaatctcgcg ttgatctgct ggcgcgggtg aaattaagtc acgataagct
      601 gaaagattta cgtgagcgca aagccagtct ggaagcccgt gcgctggaag cgttaagcaa
      661 gaacgttaat ccgtcgttga ttaacgaagt tgctgaagaa atcgcgcgcc ttgagaatct
      721 cattaccgct gaagagcaag tgttgtcgaa tctggaagtc tcccgtgatg gcgtggaaaa
      781 agcagttaca gcgacagcgc agcgtatcgc tcagtttgag cagcaaatgg aagtcgttaa
      841 agccactgaa gccatgcagc gtgcacaaca ggctgtaaca acctctaccg ttggcgcatc
      901 ttccagcgtt tcgacagcgg cagaatcctt aaaacgcctg caaacgcgtc aggccgaacg
      961 tcaggctcgc ctggatgctg ctgcacagtt ggaaaaagtc gcagacggtc gcgatcttga
     1021 tgaaaagctg gcggaagccg gaattggcgg tagcaataaa agtagcgccc aggatgtatt
     1081 agcaagactg caacgccaac agggcgagta atttttttgc cagcctcgct aaaaggctgg
     1141 caactatttt aaggataaaa tatgtctggt tttttccagc gtctgtttgg caaggataat
     1201 aagcctgcta tcgctcgcgg tccgctggga cttcatctca atagtggctt tacgctcgac
     1261 acgttagcgt ttcgtttgct ggaagatgaa ttgctgattg cgctgccggg tgaggaattt
     1321 acggtagccg ctgtcagcca catcgatctg ggcggcggta gtcagatttt ccgctactac
     1381 acttcaggcg atgaatttct gcaaatcaat accaccggcg gcgaagatat tgatgacatt
     1441 gatgatatca agctctttgt ctatgaagag agctacggta tcagtaaaga gagtcactgg
     1501 cgcgaggcga tcaacgccaa agtgatgggg gcaatgatct taaactggca ggaaaaacgc
     1561 tggcagcgat tttttaacag cgaagaaccg ggaaatatcg aacccgtcta catgctggaa
     1621 aaagtagaaa atcaaaacca tgccaaatgg gaggtccata attttaccat gggctaccaa
     1681 cgccaagtaa ccgaagatac ttacgaatat ttgctgttaa atggtgagga atcttttaac
     1741 gatctcggcg agccagagtg gttattttcg cgcgcgttgg gtgtcgatat cccgctgaca
     1801 tcacttcata ttattggtta attacaagga cgtttacaat gcatatactg gattcacttc
     1861 ttgcctttag tgcctatttt tttattggcg tggctatggt gattattttt ctgtttatct
     1921 actccaaaat tacaccgcac aacgaatggc aattaatcaa aaacaataat accgcggcgt
     1981 cactggcatt cagcggtaca ttgttgggtt acgtgatccc cttatccagt gcggcaatca
     2041 atgcggtgag tattccagac tatttcgcct ggggcgggat tgcactggtg attcagttac
     2101 tcgtttttgc tggtgtcaga ctttatatgc ccgcattaag cgaaaaaatt attaatcaca
     2161 ataccgcagc aggaatgttt atgggaaccg ccgcactggc tggtggtatt tttaacgcag
     2221 cttgtatgac atggtaatgg acgatcatgg ccagaaaacg caaatcaaga aataacagta
     2281 aaattggtca cggagcgatc agtcgcattg gaagaccgca taatcctttt gagccgcgcc
     2341 gcaatcgcta cgcacaaaaa tacttaacgt tggtgctaat gggcggtgcc gtttttttaa
     2401 tagtcaaagg atggagtgaa agtggcgatg tcgataacga cggcgacgga acgttttatt
     2461 cgacagtgca ggattgtatt gatgacggta ataatgccga catttgcgcg cgtggctgga
     2521 acaacgccaa agcggcattt tatgccgatg ttccgaagaa tatgactcag cagaattgtc
     2581 agtctaagta cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt
     2641 ctggattttt gttaagccga gttattcgta aagatcgcga tgagccgttc gtctataaca
     2701 gcggcggatc ctcatttgct tcccgccccg tctggcgcaa cacttccggt gattactcct
     2761 ggcgctccgg ttctggcaaa aaagagtctt actcttcagg cggctttacc accaaaaaag
     2821 cgtcaaccgt ttcgcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag
     2881 gctaatcatg ctgagacaca acgttcctgt gcgacgggat ctggaccaga tcgccgctaa
     2941 caacggtttc gactttcata tcatcgacaa tgaaatctat tgggacgaga gtcgggctta
     3001 ccgttttact ctgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca
     3061 gatgtgcctt gaggtggtgg atcgcgcggt taaagacgaa gagatcctga cgcaactggc
     3121 gatcccgccg ttgtactggg atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct
     3181 gtatggccgg atggattttg cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa
     3241 cgccgatacg ccgacctcgt tgtatgagtc ggcttatttc cagtggttgt ggctggagga
     3301 tgcccggcgc agcggcatta ttccgcgtga tgccgatcag tacaatgcca ttcaggaacg
     3361 cctgatttca cgctttagcg agctttatag ccgggagccg ttttatttct gctgctgtca
     3421 ggacaccgat gaagacagga ctaccgtgct gtacttgcag gactgcgctc agcaggcagg
     3481 gcaggagtcg cggtttatct acattgaaga tctcggtttg ggcgtcggcg gtgtactgac
     3541 cgatcttgat gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat
     3601 gcgtgacgat aacggtccgc tgctgtgcaa gcgtcgcgag caatgggggg agccgttatg
     3661 gaaaagtatc ttgagtaata aagggctaat gccgctgctt tggcgcttct tccctggtca
     3721 tcctaacctt cttgcatcct ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag
     3781 ctatgtgcgt aaaccgatct actcgcgcga aggcggaaac gtcaccattt tcgacggtca
     3841 gaataacgtc gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc
     3901 gtttcaacct ctgccgcgat ttggcgatag ctacacactc atcggtagct ggattgtcga
     3961 tgatgaagcg tgcggaatgg ggatccgtga agataacacg ctgatcacca aagacacctc
     4021 tcgtttcgtt ccgcattaca ttgctggcta agaatgtttt agcaatctct ttctgtcagg
     4081 aatccatggc agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac
     4141 tggcaaactc acaccgtttt taatcaacct ataccattaa ataacagcaa cttatacctg
     4201 tctgatggcg cgctctgcga agcggtaacg cgtgaaggtg ccggctggga tagcgatttt
     4261 ctagccagta ttggtcagca gttaggaacg gctgaatccc ttgaactggg gcggctggcg
     4321 aatgtgaatc cgcctgaatt attgcgctac gatgcgcaag gacgccgtct ggacgatgtg
     4381 cgttttcacc ccgcctggca cctgctgatg caggcgctat gtaccaatcg ggtgcacaat
     4441 cttgcctggg aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg
     4501 ttgcacgcac aggttgaggc agggtcgtta tgtccgataa ccatgacctt tgccgccacg
     4561 ccattgctgt tacagatgtt acccgcgccg tttcaggact ggaccacgcc gctgctgagc
     4621 gatcgctacg attctcactt attgccaggt gggcaaaaac gcggtttgtt gattggcatg
     4681 ggaatgacgg aaaagcaggg cggttccgat gtcatgagca acaccacccg cgcagagcgc
     4741 ctggaagatg gctcttatcg gctggtgggg cataaatggt ttttctcggt gccgcaaagc
     4801 gatgcgcatc tggtgctggc gcagactacg ggcggtttgt cctgcttttt tgtgccgcgc
     4861 tttttgcctg acgggcaacg caacgcgatt cgcctcgagc ggctgaaaga taagctgggt
     4921 aatcgctcta acgccagctg cgaagtggag tttcaggatg ccattggctg gttgttgggg
     4981 caggaagggg aaggaattcg tctgatcctg aaaatggggg ggatgacgcg ttttgattgc
     5041 gccctgggta gccatgccat gatgcgccgt gcattttcgc tggcgattta tcatgcacat
     5101 caacgccatg tttttggtaa tccattgatc caacagcccc ttatgcgtca tgtcttaagt
     5161 cgcatggcac ttcagcttga agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg
     5221 gaccggcgtg ccgatgccaa agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa
     5281 tttgtgatct gcaaaagagg tatgccgttt gtggccgaag cgatggaggt gctgggcggc
     5341 attggttatt gcgaggagag cgagctgccg cggctttacc gggagatgcc ggtaaacagt
     5401 atttgggaag gttccggcaa tattatgtgc ctggatgtgc tgcgcgttct caataagcaa
     5461 gcgggcgtat acgacttatt gtcggaagcg tttgtggaag tgaaagggca ggatcgctat
     5521 tttgatcgcg cggttcgtcg tttacagcag cagctgcgta agccagctga agaactgagg
     5581 cgagagatta ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct
     5641 tctccgccaa tggcgcaggc gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg
     5701 ttgtcagagc agatccagaa tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa
     5761 gcgtatacga ctgatgcgac gctggtttcg attaactaaa tgaaatatgt gaaaattgta
     5821 ggccggacaa ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga tgacactcat
     5881 gcatacagta tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta catggtaata
     5941 atgcggtacc atgacgcacc ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc
     6001 tgcggatccc ctgaaatatt gctaactgag atctgaccta tttcattcag gcccgtcacg
     6061 cagtcagcac tggcgaattc tgcagactgt gccgtggcgc tcgcaggata agtcactaat
     6121 tgcagcgagg gtgaggcaag taattgtttc atcgtcagct ccatttttcg tttccccaca
     6181 tgttgcaggg cattccatcg cggtaataaa cgcccaggct tccagccggt aatttattgt
     6241 gcacaggtaa acgcataggg acgcaaagca atgtaaagct gactatgtaa gttgtctgat
     6301 ttatttacgg tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg tttcgtccac
     6361 catgacgaca acgtaataat cagcttttgc agcgacagct ttcgctctga tttctgctaa
     6421 tgcgtcatcc ggagaacccc gaaccatcgt gcttacgctg cctattcgct gtaacccttg
     6481 cgtctggtcg cggcgaatct cttgcggatg gtccgttact ggcggtgctg gctgtggcgt
     6541 accttgcagt gcgctacagg cacttaacat caacaccaat aataaactgg caaaccggta
     6601 aataacgcta ttacgtttcc tgctaaccat agtgtagtgc cttattaatt taactttggg
     6661 ggaatgttcc cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt
     6721 aacccacatc tcaacattat gaactaatac cgccagcaga aacttgtgct aaggatttct
     6781 tcatgtcata ccagacaaaa aggagagaca gatgattgaa atagaatcac gcgagctggc
     6841 agatattccc gttcttcatg cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt
     6901 aattttttat cacggcttta cttcatccag tctggtgtat agctattttg ccgttgcgct
     6961 ggcgcaggct ggtttgcgag tgatcatgcc ggatgcgccc gatcacggta gccgttttag
     7021 tggtgacgca gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga
     7081 attcactact ttacgtgcgg caatagccga agaaaactgg ctgcttgatg accgtctggc
     7141 agtcggtggt gcgtcgatgg gcgcgatgac ggcactgggg attaccgctc gccaccccac
     7201 ggtgagatgt accgccagca tgatgggatc gggctatttt acatcactcg cccgttcact
     7261 gtttccaccg ctgatacctg aaacgacagc acagcagaat gaattcaata acattgtcgc
     7321 gccgctggca gagtgggaag cgacaaacca cctggaacaa cttagtgaca gacctctact
     7381 gctgtggcat ggcctcgacg atgacgttgt gcctgccgac gaatcactac gtttgcagca
     7441 ggccttaagc gagacgggac gggataaact gctaacctgt tcatggcagc caggcgtgcg
     7501 tcaccgcatt acgcctgagg cgttagatgc tgcagtgaca tttttccgcc agcatcttta
     7561 aacacgcaga atgctgaccc cttgcgcttc cagttgttgc aggatttccg ggttagcatt
     7621 tttgccggtg atcagcatat caatttgatc ggcacggcta aaaagcattc ccgcgcgttc
     7681 gccaatctta ctgctatcaa ccagtaccac cagtttccct accacgctca gcatcttctg
     7741 ctctgccatt gccgtcagca tatcggtttt atacaaccct tctgctgtca gtcctttgcc
     7801 gctggtaaac atccagtgcc cggcatagag actgttttcg ctgccctgcg ggcttaaagt
     7861 gatggactga cttttgttgt actgtccgcc cataatgatc acgctgtcat gttcttgatc
     7921 gatcaggtaa tttgccagcg gtagataatt agtgatgatt tgcactggct tgccacacat
     7981 ttcccgccca agcagaaacg cggtggagcc gcagttgatg actacgcttt cgcccggatt
     8041 aaccagctgc gaggccgctt tagcgatacg tactttttca tcgtgattct gcgcctgatg
     8101 cagattcatc ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac
     8161 ttttttcagt ttgccgcttt cgtcaagttt attgatatcg cgtcgcgcag tggcaggcga
     8221 aattcccaga cgctcaacga ctttctcaac ggtcacaaag cccaattgtg cgagcatttc
     8281 caggaggatt tgatgtcttt gtgcttcagt catgagctat tccgataaaa tttgattttt
     8341 ttagatgata tttgaaatag ccaggaaata ctacgccgga tagcgcgaac ttctccacgc
     8401 tatccggtat aaacgacaga ttacaggaat gacttgaacg gcagatccgg ttcaatagtg
     8461 aagcaatcat cgaaaccgcg cggatagtgg tactcgaagt tgtctttatc cagcggccag
     8521 gtaaatttgc cgccaacctg ccagataaac ggcttgaagc catacttcag acgatctttt
     8581 ttcatctccc acagcacgcg gatctcttgc ggatcggcct ggaagtttga ccagatgtcg
     8641 tggtggaacg ggataaccac tttcgcattc agggcttcac ccatacgcag catatcggcg
     8701 ctggtcattt tgtcggtaat accgcgcggg ttctcaccat acgatcctaa cgccacatcg
     8761 atctgatgtt cgttaccgtg cttcgcataa tagttggagt agtgggaatc gccactgtga
     8821 tatagggaac cgccaggggt tttgaacagg tagtttactg cgcgatcgtc catgccatct
     8881 ggcagtacgc cagccgcttt ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa
     8941 gcgtcaagcg catgaatttc aatgtctttc acttttacta catcgcccgg tttgaccacg
     9001 atgcaacgct ctttcggtac gccccagcca atccacaaat ccacacaggt tttcggtcca
     9061 atgaacggta cgtcatcagc acagttctgc atcacggcag cagcaacgtt aacgtcgata
     9121 tgatcgttgt gatcgtgagt cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca
     9181 agaacaaacg gggtggtgcg caggtttggc tgcagttttt tcacgccagc catgcgctgc
     9241 atctggtgac cctgtttcat taacgggtta ccgtgacttt gtttgccagt gccgcaccag
     9301 aaatcaacgc aaacgttggt gccaccttcc gatttcaacc agatcccggt gcagccaagc
     9361 caccacatcg caaatgtgcc tggagcgacc tgttcttgtt caatttcttc attcaaccag
     9421 ctaccccact ccgggaaagt gctcaggatc caggattcac gggtgatact tttcacttta
     9481 ctcatcgcca tttaccttca tgattgttca attcgaatca atatgtgatt ggttttgatt
     9541 aatcctgaca ctattttttc aggaaggcaa tgaccatttt ttgacttttt gccagggaag
     9601 tggttgttga ttttgagtgt ggaaagattt aatggaatgt gtaattcaat taactaaatg
     9661 aatttaaatg aatagttgtt tcgttgtgtg aatcccattc tatccatgtg gaattatttg
     9721 cgggtcgcgt cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt
     9781 gagtgcacaa cattccgggt gtgtggaata cccggttacc tcttcttcag gagatcgtta
     9841 tggagatcct ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt
     9901 tgttgctggg tattgtgacc tgtctgggct acatcctact gcgcaaaagt gtcagcgtta
     9961 ttattaaagg cacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca
    10021 tcctcaccag caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg
    10081 gcgcaatttc cgatacttac gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct
    10141 atagctgggt aggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc
    10201 tgcgtcgcat taccggtatt cgtaccatca tgctgactgg tcatatcatg ttccagcagg
    10261 cggggctgat tgccgttacg ttgtttattt tcggctactc catgtggaca accattatct
    10321 gcacggcgat tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga
    10381 ctcaggaagt gacggacggc tgcggtttct ccatcggtca ccagcaacag tttgcatcat
    10441 ggattgccta taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca
    10501 aactgccagg ctggctgaat attttccacg acaacatcgt ctccacggcg attgtgatga
    10561 ccatcttctt tggtgccatt ctgctctcct tcggtatcga caccgtgcag gcgatggcag
    10621 gcaaagtgaa ctggacggtg tacatcctgc aaactggttt ctccttcgcg gtggcgatct
    10681 tcatcatcac tcagggcgtg cgcatgtttg tggcggaact ctctgaagca tttaacggta
    10741 tctcccagcg cctgatccct ggtgcggttc tggcgattga ctgtgcggct atctatagct
    10801 tcgcgccgaa cgccgtggtt tggggcttta tgtggggcac catcggtcag ctgattgcgg
    10861 ttggcatcct ggtcgcctgc ggttcctcga tcctgattat tcccggcttt atcccgatgt
    10921 tcttctccaa cgccaccatc ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc
    10981 tgaagatatg tctggtgatg gggatgattg aaatctttgg ctgcgtctgg gcggtgaaac
    11041 tcaccggtat gagcgcctgg atgggcatgg cggactggtc gattctggca ccgccgatga
    11101 tgcagggctt cttctccatc ggtatcgcct ttatggccgt catcattgta attgcactgg
    11161 cttatatgtt cttcgctggc cgcgcactgc gcgcagaaga agatgcagaa aaacaactgg
    11221 cagaacagtc tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg
    11281 gcaacggaca aggcagttcc atgatcatga agatgaaagt ggaccagttt ttaacccaat
    11341 caaacattga ccacacggta aatagctgcg cggttggcga atacaaaagc gagttgagcg
    11401 gcgcggatat catcatcgct tctacgcaca ttgcgggcga aatcaccgtg accggcaaca
    11461 aatacgtggt tggcgtgcgc aacatgctct ctcctgccga ctttggcccg aaactgctgg
    11521 aagtgatcaa agcgcatttc ccgcaggatg tgaagtaagg acgcgccatg aaattacgtg
    11581 attcgctggc ggagaataaa tccatccgcc tgcaggctga agctgagaca tggcaggacg
    11641 cagtaaaaat cggcgttgac ctgctggtgg cggcggatgt tgtcgagccg cgttactacc
    11701 aggcgattct ggatgcggtt gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg
    11761 caatgccgca cggtcgcccg gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc
    11821 tgaaaaagcc gctggagttc aaccacgaag ataacgaccc ggtggatatc ctcatcacca
    11881 tggcggcggt cgatgccaac acgcaccagg aagtgggcat catgcagatc gtcaacctgt
    11941 ttgaagatga agaaaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg
    12001 atctcattga ccgcaccaac gcggcagctt aagaaggaat tgaacatgtc attaccgatg
    12061 ttgcaagtcg cgctggacaa ccagacaatg gatagcgcct acgaaaccac tcgcctgatt
    12121 gccgaagaag tcgacattat cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt
    12181 gcggttcgcg atctgaaagc gctctacccg cacaaaatcg tactggctga cgccaaaatt
    12241 gccgatgcag gcaaaatcct ttcgcgtatg tgcttcgaag ccaacgctga ctgggtgacc
    12301 gtgatttgtt gtgcggatat caacaccgcc aaaggtgcgc tggacgtggc aaaagagttt
    12361 aacggcgacg tgcagatcga actgaccggt tactggacct gggaacaggc gcaacagtgg
    12421 cgcgatgcag gtattcagca ggtggtttat caccgcagcc gtgacgcgca ggccgctggc
    12481 gtggcatggg gtgaagccga tatcactgcg atcaaacgtc tttccgatat gggcttcaaa
    12541 gtcaccgtca ccggtggcct ggcgctggaa gatctgccgc tgttcaaagg cattccaatt
    12601 catgtgttta tcgccggtcg cagcatccgt gatgccgctt ctccggttga agccgcgcgt
    12661 cagttcaaac gttccatcgc cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa
    12721 atcccgcttg gcatctatga aaaagcgctc cccgccgggg agtgctggct ggaacgcctg
    12781 cgactggcaa aaacgttggg cttcgatttt gtcgagatgt cggtagatga aactgacgag
    12841 cgcctgtcgc gcctcgactg gagccgcgag cagcgtctgg cgctggtgaa tgcgattgtt
    12901 gaaaccggcg tgcgtgtgcc gtccatgtgc ctttctgccc atcgtcgttt tccgttgggc
    12961 agcgaagatg acgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc catccagttc
    13021 gcccaggacg ttggcattcg cgtgatccag ctggcgggct atgacgttta ctatcaggaa
    13081 gccaataacg aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggcg
    13141 agccgcgcgc aggtgacgct ggcgatggag atcatggatt atccgttgat gaactccatc
    13201 agcaaggcgc tgggttacgc gcactatctc aacaacccgt ggttccagct ctacccggat
    13261 atcggcaacc tgtcggcgtg ggacaacgat gtgcagatgg agttgcaggc cggaatcggg
    13321 catatcgtcg cggtacatgt gaaagacacc aaacctggcg tcttcaaaaa cgtgccgttt
    13381 ggcgaaggtg tagtggattt cgaacgttgt ttcgaaacgc tcaaacagag tggctattgc
    13441 gggccgtacc tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtggcg
    13501 aaagcgcgtg attgggtgaa agcgcgcatg gcgaaagcgg gcatggtgga ggcggcataa
    13561 tgcaaaagct aaaacagcag gtatttgaag ccaacatgga tctgccgcgc tacgggctgg
    13621 tgacctttac ctggggcaac gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca
    13681 agcccagcgg cgtcgcctac gaaaccatga aagcagacga tatggtggtg gtcgatatga
    13741 gcggtaaggt ggtggaaggg gagtatcgcc catcttccga caccgcgacg catcttgaac
    13801 tctaccgtcg ttatccgtcg cttggcggca ttgtccatac ccactccact catgctaccg
    13861 catgggcgca ggcggggctg gcgatcccgg cgttaggcac cacgcacgct gactacttct
    13921 ttggtgacat cccgtgtacg cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac
    13981 tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc cgagccgctg catacgccgg
    14041 gaattgtggt gtatcagcac ggaccgttcg cctgggggaa agatgcccac gacgcggtgc
    14101 ataacgcggt agtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaatc
    14161 cacaactcaa tcacatcgac agcttcctga tgaataaaca cttcatgcgt aagcatggtc
    14221 ctaacgctta ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cttcgggaga
    14281 taactggtct aattccgcag ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca
    14341 aactatggca atgtttcgtt ggttatacct ggtgctagcg ataaatatcc gcgctggcgt
    14401 gcatattgcc gttactcccc ggttcccgca tcagaattac gtggtagtac gttgcgccct
    14461 gcgcatctgt ttcttcattt aatgcctgac gtgcttcact ttcagtggcg aaattatgat
    14521 tgatataaat cacgcctaag ctttgcacat cgtccatatt tctggcctgg tggttattga
    14581 tttcaatggc tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaactc
    14641 gactgaacat agatacctcc tcgacggctg actttgtgtg ctctcctctg tgatgatctt
    14701 ctgatttaat tttaatcaat gataaagaag ttgatggtga ccatttctga tgcagttgtt
    14761 caaaaaaaca ctgtgacgga atgtgatgaa cttcaaatca gggagttagt tcttttgagc
    14821 gaaagatgag atttatttgt cggctggcct tgagtttagc ggggcataca agtactataa
    14881 cgcgtcattt ttcagccgac ctttaacacg ttccttgcct ccccgggatt cggctgaccc
    14941 agacaggagg ctgaataatc cgtaaggagc aattcgatgc gtcattacga aatcgttttt
    15001 atggtccatc ctgaccagag cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc
    15061 atcactggtg cagaaggcaa gatccaccgt ctggaagact ggggccgccg tcagctggct
    15121 tacccgatca acaaactgca caaagcacac tacgttctga tgaacgttga agctccgcag
    15181 gaagtgatcg atgagctgga aactaccttc cgcttcaacg atgccgttat ccgcagcatg
    15241 gttatgcgta ccaagcacgc tgttaccgaa gcatctccga tggttaaagc gaaagacgag
    15301 cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg atgatgctga agctggggat
    15361 tctgaagagt aatttctgat gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact
    15421 ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc agttcgtgct tgagcatcgt
    15481 tctgtgcagg aggaagccgg ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt
    15541 agcggacacg aaaaccaggc cattactcac agtataacgg tcggcagtcg cataaccgtt
    15601 caggggttca tttcatgcca caaggcaaag aacggactga gcaaaatggt tttgcatgcc
    15661 gagcagattg aattgataga ttctggagac tagccatatg gcacgttatt tccgtcgtcg
    15721 caagttctgc cgtttcaccg cggaaggcgt tcaagagatc gactataaag atatcgctac
    15781 gctgaaaaac tacatcaccg aaagcggtaa gattgtccca agccgtatca ccggtacccg
    15841 tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct
    15901 gccgtacact gatcgccatc agtaatcggt cacggtccat taatacgact ttgagaggat
    15961 aaggtaatgc aagttattct gcttgataaa gtagcaaacc tgggtagcct gggtgatcag
    16021 gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac cgcagggtaa agctgttcca
    16081 gctaccaaga aaaacattga attcttcgaa gcacgtcgcg ctgaactgga agctaaactg
    16141 gctgaagttc tggcagctgc taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt
    16201 accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg gttccatcgg tactcgcgac
    16261 atcgctgacg ctgtaactgc agctggcgtt gaagtggcta agagcgaagt tcgtctgccg
    16321 aacggcgttc tgcgtaccac tggcgaacac gaagtgagct tccaggttca cagcgaagta
    16381 ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg ttattcaacg agacgtaaaa
    16441 aaacgccgac cattggtcgg cgttttgctt ttgtagcgag cacttgcaga tatcgataag
    16501 ggccacaata agcagcccta cgaagataat tacttagccg cctgaatatc agccagcatt
    16561 ggggcaaggg ctgggttatc cttagcgaag tttgcgtaca ttggctgtac tttctcgata
    16621 aacggagctt tatctacctg gtagactttc acgcccattt tatccagttt tgcaaattct
    16681 tgtttctctt cctcgctcca caagtctttc atgtagctca ttgattcttt agctgctttt
    16741 aatataactg cctgattttc cgggctaagt ttatcaaacg cagcattgct catcagaacc
    16801 acatcgggaa ccatagtgtg ttcgtcaagg ctgaaggatt ttgccacttc accgtgacgc
    16861 atggtagtca gcgccatcac gctgttttct gccatatcga ccacaccctg ctgtaaggct
    16921 gtatacaact cgccgtaatc cagtggcgtc gggttaccgc ccatgacttt gatcatttca
    16981 actgcgccag ggcttggctg gacacggact ttcatgcctt tcagatcgtc tggtttcagt
    17041 actggtttat tgccatagaa actgcgggcg cctccatcat aaaaagtcag cccgaaataa
    17101 cctttgcttt tcgttgagtc gaggattttt ctcccgatat cgccctgcat gacctgataa
    17161 taatgatcac gatcgcggaa caaataaggc aggctaaata atttatagga attttcaaat
    17221 gcttccattt ctgccgcatt ggtttttacc aggggaatag cgccagaacg aatcagctcc
    17281 attgtttctc gttgagtacc caatgttcca tttggataaa tacgaatagt aatatcaccg
    17341 ttagagtact ctttgctctt tttcgcaaag aactccatcg ctttatgaac aggatgagac
    17401 ttatcctgat tatgactcag ttttaatgtc actgcccagg actggcttgc ggataacata
    17461 agaacagcac cgataatgcc agcggatact tttattttca tagatgttcc ttttctaata
    17521 aatatggcgg caggtgccgc cagtgaatgc gcatcagtag cccagtgctc ttggcaatgc
    17581 catactgatt tgcggaatat aggtcaccat tgccagcacc aataccatca cggcatagaa
    17641 tggcagcata ggtttgataa ttttattaat cggtaattta gatacactac aaccgacaaa
    17701 taacacactg cctacaggcg gagtacaaat accgatacac atgttcagta ccatgacaat
    17761 accaaagtga atcgggtcca tgcccagttc ggtgacaacc ggtaaaaata tcggcgtaaa
    17821 aatcagtacc gccggtgtca tatccataaa agtgccgata atcaacagga taatattgat
    17881 aataagcagg attattattg ggttgtcaga aatagtgtta agcaaatcga gaatcaggaa
    17941 gggaacgtca gcgtttgaca ttgcccatga cattcccatc gaagaaccaa ttaataacaa
    18001 gacaattgca gtggtaataa cggattccag aaatatttta ggtaaatctt taactgagat
    18061 ttcacgataa agaaccagtg caaggaaaag ggtataaatt accgcaattg cggaggcttc
    18121 ggttggagta aaaataccgc ttagtacgcc acccataatt aacacaatca ggaaaatact
    18181 ggggatagcc atccatatcg caacgccagc ctggcgcaaa gtcggacgtt ctggttttgg
    18241 ataaccgcga cgtacagcga taataccggc tattaccatc agtgccagac ccagcaaaat
    18301 ccccggaaca taacccgcca ggaacagtgc ggcaatagaa gtaccaccac tgaccagtga
    18361 atagacgatt aacgtattac ttggtggaat aagcaaaccc gtcggggcag aggcaacgtt
    18421 aaccgcagta ctgaatgccg ggtcgtagcc ctctttaacc tgttgcggat gcatgacgcc
    18481 gcccatcgca gccgccgaag ccacggctga accagaaatt gccccgaaca gcatattggc
    18541 gagaatatta cagtgggcaa gtgagcctgg cagacgaccg ccgagaatac gggcaaagtt
    18601 gattaatcgt cgcgctattc ctccatgatt cataatgttg ccagacaaga taaaaaatgg
    18661 gattgccaac aacgtaaaac tatctaaccc ggaggctaac ttttgggaaa ccacactcaa
    18721 tgctgagttg aaatcgagtt gtgtggaaat tgatgccagt gtcgataaac caatggcata
    18781 aacaataggt acacctaatg ccaacataaa aaagaaggca ccaaataaca caataatcgg
    18841 taaccagtaa tccataattt ctccattcct actatggttg gttgttgttt gttatcttta
    18901 gagtggttgt ttaagttcgg ttaaaaccat atctatggtg tatataataa taagtacacc
    18961 actaatcggg atgctcatat agatatatcc catttgccag cctaaagcgg gcgaggtctg
    19021 acctgaatcg aatgtatcca tcgttaatag cccaccacca tataccataa tgatggttgc
    19081 gaagataata acgataatct gaatacacaa aaataaatga cgtcgggctt ttcctttcag
    19141 catcataggt agcaggtcaa tcgccagatg ccggttttgc cctgcggtgt atgccccacc
    19201 aaaaagtcca agacaaataa agagcaaacg gctgagttca tctgtaaaaa tactggggct
    19261 agtaaatata aatcgtgcag ccacctgcca cgtcacacat gccacgatga tcgccaaaat
    19321 ggtaatgttc aatgctgcaa gcagagcatt aatagatcta attatatttc gcatagtgat
    19381 taacctttgc aatatcattc atcctgtggc tggtaaattg tcccattaag aaaaccaggc
    19441 cagttttttt caaaccaaat acctcggata ataggaaact cacgaacagg tgtttctctg
    19501 acgtgaaaga taccgttgca ataatgaata agtagtttat cttttttatc ctcagtacgc
    19561 tctgaacgaa agcgccaggc atcacaaaaa ccatttcgtg tttgcggaat agaatttcca
    19621 tccctgtcga gaataatacg tgctccccga ctaccaccac ctgcgtcaat ataatgcgtt
    19681 aattgcgtta atacggcggc agaagtcagt gtcatatgcc gccacataaa cagctcggca
    19741 acttcaccca catgtttgat agccaatcca tgccgttgga caaattcgct caatagcagg
    19801 gcatcgcgag tggcgcgtcg gactttatcg gcatggcaaa taaatcccgc atggtcagac
    19861 attcgtgcct gaattttttc tctcacaacc gacaacggca tcccggtccc gttatcgtgc
    19921 gcctgagtaa tgatctctct tatagaagcc agtgtaggag ctaccagctg tgctatatct
    19981 ccatcaatat tccgcttttg cgtgcaacca ataaaacgtg ccagacgaac agcaaaaacc
    20041 tgcccggcat tcaatgctgc accaccaggg cgagtgacgc cgtgtgtgcc agcaacttcc
    20101 cccacggcga aacagccggg cagggaggtt tgtccccaga tatccacttc aatgccgcca
    20161 ttcatatgct gattattcat ggcaaactgc aaaggctgtg tggtgagatc gtaaccgtgc
    20221 atcttataca gcgagataga cagcggattc attcgttgca gccgttcgat gggcgatggt
    20281 gccagagcgt cgttattttc cagatacgcg cgaacgtcgt cgtccagtcg atctaatgag
    20341 aacggcaggt cacccggcac cgcttcagga ttgcgattga aatccataaa tacctgacgc
    20401 cctgattgct gctcttgcgc tactgccata tctaacaagc tggagccaaa atccatcacc
    20461 cgcgtggcgt ggaacggcca ctgatagcct ttacggaaaa tgtttgaagc cagctcctgg
    20521 gtggtgcgat agtaatccgc gaggaagtta tactcgttac cctcagcatc cacggaatag
    20581 atatacggga tcacctgtac ataggtgccg gataaattcc acggaaacgt gctacgcggc
    20641 gtgcctatac caaactggct ttcggtcaga ttggttagcg tcagaccttc ttccagcgcc
    20701 agccccagcg agccgaagca tttatgtgga taaacactgt cgcgataaag ctcgcccggt
    20761 ccgcccgttg ccagtaccac attgggcgca gtcacaattg ccagccccca tgggttatgg
    20821 gcgcgatgac cggtcgcgag gattgcccct gccacgcggt cttcgccgtt ttcgtcacgc
    20881 tgatgcagca gtttaatcac tgttgcactg gtcaacactg gaatggcgag gcgctgtact
    20941 tcttccaaca gcactttcac cattaacctt gaggtacgcg gcccacacga ggttgcacgc
    21001 ccggcttcgt catggtcggt ttgataacga agaatcgcgc catagcgatc ttccggtaat
    21061 tccagaccaa gatattgcaa cccgcctaga gtgtgcagag aacctaccgc ttcgacataa
    21121 gcggtgtcgt gatccatcgc cccaccgctc gccagtgcct ctgccagttt ggtgaagttg
    21181 tcgccgttgc ccgcggtagc ggcggtaaat agtgtctgtt tatcggaacc agaacatgcc
    21241 gacgtcccca tatataaccc ggcggtagcg atcagcacat tttgctggcg gcgtttaagt
    21301 tcaactgccg cacgtaatcc tgctgcacca ctgccgacta ccagggcatc tgtttgccag
    21361 agtgggtaat caacaccgtc taccgtgaca acccatgcgg gctctgcctg tgcaaaaccc
    21421 tttgggaaag taacgtgcgt cagcgcatca aggatggtgt catcaagctg actcatgcat
    21481 cctccatcgg cgtaaacagg ctactttcca tcaggcgtgg gtgcttgata attggacgga
    21541 aagccatata cggcagaatg tcgcgctcaa tctccacacc cggcgcgatt tcaattaatt
    21601 caacgccttc tgccgttaac tggaatactg cgcgttcagt gatatacaac acttcctgac
    21661 cactttgctg tgcgtaggcg gcgctaaaag tgattttttc cacctgggta accagtttag
    21721 ggatttgacc tgactgggtg atatgcaagc catctggcgt tacatcaatc ttgctacctt
    21781 tggcgtcgaa cgtgccgcag aacaccactt tacgggcgtt ttgcgcaatt tcgagaaaac
    21841 cgccgggacc aatgagattg ccattgagat gggatacgtt gacgttaccg tactgatcca
    21901 tctctcccat gccgaggaag gtgatatcga ttcctccgcc actgtagaac tcaaattgat
    21961 cgagtgatgg aataatggca tcggcgttgc gggcgcagcc gaaaaacgca tcatccagca
    22021 tcataccgtt atgcacgcct tgctcgacgc tcagccataa actttggtaa ggcacgcctt
    22081 cgcgcagcgc tacgcctgga atacccccag gaataccaaa gccaaagttg gttgaagcgc
    22141 cagctaccag ttcgcgtgcc gcccgacggg caatcaggcg acgtaccgga tgactaacca
    22201 gttcaatggc gtcgttagaa ctcagacggc gatgttggcc gctgatggtc aggtcgtaac
    22261 cgccaagata ggtttgtggt tgttcgcggt gttcaacgat gccatcaacc agaatgcccg
    22321 gtaatttgac ccggcgtggt tcaatcgcgc cagcttccag cacatcgcgt acctgtacga
    22381 acactttgcc gccgctgttg tgtgctgcca gcgccatgga atagctatcc atatcaatcg
    22441 cttcttcttc aaggctgacg ttgcctcgag gatcggcata agtgccacgc aaaatcgcgt
    22501 aatccacttt gaaagggcga taacgaagtt tggtttcacc gtcgatttca atcagttcta
    22561 ccaggtcgtc ggtagtgcat tcattcgact taccgccgcc attgcgtgga tcaacaaacg
    22621 atcccagccc aacgtgggta aaaagccccg gacgtccggc gccgatctcc cgtagcagtg
    22681 cctgaatcac gccaccagga aagcagtagg cttcaatcgt gttatttttt accagcgcct
    22741 gcattttggg cgaccaggta aagtgcccgg caataatgcg tttgagcatc tctgcgtgcg
    22801 caaaacggtt agtgcctttg cagtcgcgat cgccaatacc cagcgagtgg attagcgtca
    22861 ggtcgcgagg gtgtccggtt tgcaggaaac gcgcttcaat agcagccaga atatggtcgg
    22921 cttccaccat gccgccgccg ttaccgctaa tggcaatggt tgcgccgtcc tgaatttgcg
    22981 ccgccagtgc ctcggcggtt gttatcttac gcatacaatt tccttattga ttatggaagc
    23041 tcacgctact tttttcggca aaggcgcgcg tcgcctgctg gcaatcctga gtggcggaca
    23101 ttaatccggc cagtagctgg tgcgggtaat cggcatgttt attttccagt gccagaatca
    23161 gttgcttaat atgcgccagt gccaccggac cgcatttttc cagctgcgct aactggttcg
    23221 ccatccagct ttccacctgt tctatttcta ccacttcatc aattaaattc gccgcctggg
    23281 cttcctgcgc cgtcaggcgt ttacccagca tcagcatctg gcgaccgacc accggaccaa
    23341 cctgattaag cacacgttca atgcccatcc agccaggcac catgccgagc atcacttccg
    23401 ggttagagaa tttcgcgccg gggcgggcaa tacgaatatc gcagcacaag gccagttcaa
    23461 taccaccgcc gatggtatga ccgttgagat tcgcaacggt taattggggt aagtttcgca
    23521 ggcgattaaa aacgtcatta ccgcgtttaa tccattttct gcccatatct aatggcgaat
    23581 aggccgacca cgatttaata tcgccgcccg cacaaaagaa tttatcgccg gttgccgtta
    23641 attcgactaa tcgtaatgtg gtgtcgtttt caatttcatt taaataatgg tcgagagatt
    23701 cgatcatctc ttcgttaata gcatgacatt tatcttcacg gttcaatgtc aggcggcagg
    23761 aagccgccac tctgctgaac agaacgggtt ggtcactcat gatcattcct taacaataat
    23821 gcgcgatgcg atcgacgcta atatccagcg tgctgttaat atctttctgc caccagtaag
    23881 gcgagaatat ttccagttca atagcaccat taaatccggc gttttcaacc agccgacgaa
    23941 ttgacggaat attgatgacg ccatctcccg gcataccacg gtcattgacc agatcagtgg
    24001 tcggcactaa ccagtcggaa acatgaaacg cgaggatgcg ttttccggca cgcaggatct
    24061 gactggcgag atccgggtcc caccaaacat ggtagacatc taccgccacg ccgaggccaa
    24121 attcgccatc gggatcgagt tcatcacacc agtccagcgc ctgacgtaac gtgcacagac
    24181 aggaacgatc ggctgcggtc atcgggtgca gcggctccag tgcaattggc acgccgacat
    24241 ctttcgaatg tggcagcaac tggcgaatcc cttgtttcac ttgttcccgc gcttcatata
    24301 gatctttagt gcccgttggc agaccgccaa ccacctgcat ataacaggcg gcattcagta
    24361 cagcggcgtc gtccagcgcc cggcggttat cgtcaatcgc cagtttgcgc tccgccagcg
    24421 tcggtgcggt gtagtaggtg ctacggcaca aaccggaaac gttcatctta ctggcggcaa
    24481 gctgacgggc gatctgctgg agatcttcgc cctgcaactc cctgcgccag ggggcaattg
    24541 cgccaatacc gcgtgcagcg caggcatcaa taatggcggg tagcggcgtt ttatagccca
    24601 gtgttgcggt attgatactg aataaatccg ggcgttgtaa cagatctctc atgattcagc
    24661 ctcagaagcg cggcatactt aagccgccgt cgatggcgac cgcctgacca caggtgtaaa
    24721 tcagtttacc ttccgccatc gcgcggacgg tggaagcaat atcagccgga taaccccaac
    24781 gtccccacgg caccagacct ttggcaatca gttcgtcgta ataagccgtt gccggaatag
    24841 tcatatcagt tttgatgaga cctgggcgca cttcataaac gccaatttgt tcattacaaa
    24901 ggcgagcagc aaatagacga gcagcggcag aaacggcggt tttggcaatg gtgtattcgc
    24961 cgcgattcat cgccagcatg atggcgttga tggaactgac gaaaataatc gagcggtgcg
    25021 gcagttccgc ttccggtttc ggctgtgcca gtaagcgctt actgaatgcc tgagcaagga
    25081 agaacggcgc gcgggtgtta atggcaatgt tttgatcgaa gctgtctggt tcgaggtcga
    25141 ggaggtcacc gcgttttttc actgaaatcc cggcgttatt gagcaggcag tccagacgcc
    25201 cccactggtt ttgcgccgcg tcgagcatct cttcatgcaa ggagagatcg ccgacatcgg
    25261 cagggaagca aatgacttcc acgccttctg ccaaacattc ttgttgtgtg atatgcagtt
    25321 tttctacgct gtcggcatcg ggacgatcgt taatcagcag gttaaacccg ccacgagcaa
    25381 gctccagcgc acagcccttt ccaataccac gagcggctcc ggtaatcacg gcaactggac
    25441 gagtggattt catacaataa ccctcttttg aatattgttt tctttagtga tgattagctt
    25501 gcgactggca ggtaaaaaga tcaaccatct agttggttga tctgcgagag tgatcacagg
    25561 taatttgata actcaatgaa attacgactt ttatcctttt tgctgtcgct gagatcaaat
    25621 ccggttatga tagccatcac gaagaggaag tggggaagtg gaatgtatct tgataccagt
    25681 cacgcacaaa acctgaagga aaaattgctg ctatgtgcgg ttaatgaatt tgccgaatat
    25741 ggttatgaag gggccagagt agataatatt gttaaggcag cagggtgtag taaacagacg
    25801 gtttatcacc actttggtaa taaagaaaat ttgtttattg aagtactgga atatacctgg
    25861 aacgatattc gcgaaaaaga gagagagctt gacatttcag ggctttctcc ggttattgcg
    25921 atcgagaaaa taattgattt tacttgggat tattacatca ataatccgtg gtttttaaaa
    25981 attgtccata gtgaaaacca aagtaagggt gtgcattata aaaaatcaaa acgcctcgct
    26041 gaaattaacc atgcacattt gcagtcaatg gcttcgttat tagaagaagg gcagcgacag
    26101 aaaaccttta agaaagatat tgatccctta caaatcaata ttaatatcgc cgcactgggt
    26161 gcatattatt taataaacca gtacacactg gggatggttt atcacattag catggtttca
    26221 cctgaggctt tggaagccag acgcagagtg atcaaagaga ccatcctgag ctggcttttg
    26281 gtgaaccctg cccgtgggta atcagcgtgc gcggatgaaa ttaccatcag gttggcgggt
    26341 gaacagtact tgctgattat ttcccgtatc aatggttaag ccggtaacca ccccattcgc
    26401 attttgacgg attttaacaa tctgaccatt ttgcaggtta ctgagcggtt tatccgcgcc
    26461 ctcgacctgg gccatagcgt acacatctgt aggcggcaaa ttatggtcgc ggaacagctg
    26521 ggcaagggtc ttgcctggtt ctacgcgata tgtccgccac tgttgatcga taccactttc
    26581 cggttggaaa gattgtgtct ctgcaacagg tggctcttca ggttgacctt cctgaatagg
    26641 ttccggcgcg accggggcga cctgatccgg atcgttttgc ggcgtcacta aatgtgcctg
    26701 tagctgtgct tcacctgggg ggggtgattg catatctaac tgcgcttcgc gggtgacaac
    26761 aggcatctgg cgtgtttcat ctccaggcag aagaataccg ataatcagca ctattgccgc
    26821 aataatgatt ccccggcgat gcatcggcgg caacgggtcc atgaggcgaa agttatctgg
    26881 tgcgtgccag attttcacca gagttggttt tagttcaaag cgcccaggca tggttttcct
    26941 ccagctccgc gtcgtgttcc tcaatcatag cgcagtaata agctaacgct atgatgtccg
    27001 tggtattacc gctgtttttg tattcatgcg gattatccct attgtttctg tttccctggg
    27061 atttgtcact catcccgcga caaagtttta ccagaagaag cgtggctgat atcctgcccg
    27121 ctactttata ccctaagaaa ggaaatacga tgaccacccc aacttttgac accatcgaag
    27181 cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca actgagtgaa tctggcctgg
    27241 aagggctgct gccagaagca ctggttgcag gtattgccga tgcgctggaa ggcaaacatc
    27301 cggctgttcc ggttgatgtg gtgcatcgcg cgctgcgtga aatccacgag cgcgccgatg
    27361 ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa
    27421 acgccaaaaa agaaggtgtg aatagcaccg aatctggcct gcaattccgc gtgatcaacc
    27481 agggtgaagg cgcaattccg gcacgtaccg accgcgttcg tgttcattac accggtaaac
    27541 tgatcgacgg caccgtgttt gacagctccg ttgctcgtgg tgaacccgct gaatttccgg
    27601 ttaatggcgt gatccctggc tggattgaag cactgactct gatgccggta ggttctaaat
    27661 gggaactgac tatcccgcag gaactggcat atggcgagcg cggcgcaggc gcatccatcc
    27721 ctccgttcag caccctggtg tttgaagtcg aactgctgga aatcctctaa gcagcgcatt
    27781 ctgttcccct cgaaccagag gggagcaggc attcagcaat aaacccttca gtttgccaaa
    27841 cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt
    27901 tatcaccgtg atatgacact cactttaaac ataaaattaa cattagatct aaatcttagt
    27961 attcatcccg cgtattgtta cctaatatcg atgagtcccg atacagattc gtcgtatcat
    28021 agactgacta aaggccgtag agcctgaaca acacagacag gtacaggaag aaaaaaacat
    28081 ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg gctgaacagt cgctacggcg
    28141 caatctcaca aaccgacata ttcagcttat tgccattggc ggtgccattg gtacggggtt
    28201 gtttatgggg tctggcaaaa cgattagcct tgccgggccg tcgatcattt tcgtttatat
    28261 gatcattggt tttatgctct ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa
    28321 tctggaatac aaatctttta gtgacttcgc ttccgattta ctcgggccgt gggcaggata
    28381 tttcaccggc tggacttact ggttctgctg ggttgtaacc ggtatggcag acgtggtggc
    28441 gatcactgct tatgctcagt tctggttccc cgatctctcc gactgggtcg cctcgctggc
    28501 ggtgatagtg ctgctgctga cgctcaatct cgccaccgtg aaaatgttcg gtgagatgga
    28561 gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg ctgattgtcg tcggcctggt
    28621 tatggtggcg atgcactttc agtcaccgac tggtgtggaa gcgtcattcg cgcatttgtg
    28681 gaatgatggc ggctggttcc cgaaaggttt aagtggcttc tttgccggat tccagatagc
    28741 ggttttcgct ttcgtaggga ttgagctggt aggtacaacc gctgcggaaa ccaaagatcc
    28801 ggagaaatca ctgccgcgtg caattaactc tattccgatc cgtatcatta tgttctacgt
    28861 cttcgcgctg attgtaatta tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag
    28921 cccgtttgtt gaactgttcg tgttggtagg gctgcctgct gccgcaagcg tgatcaactt
    28981 tgtggtgctg acctctgcgg cgtcttccgc taacagcggc gtcttctcta ccagccgtat
    29041 gctgtttggt ctggcgcagg aaggtgtagc accgaaagcg ttcgctaaac tttctaagcg
    29101 cgcagtaccc gcgaaagggc tgaccttctc ctgcatctgt ctgctcggcg gcgtggtgat
    29161 gctgtatgtg aatcccagcg tgattggcgc gttcacgatg atcacgacgg tttccgcgat
    29221 tctgtttatg ttcgtctgga cgattatcct ttgctcgtac ctggtgtatc gcaaacagcg
    29281 tcctcatctg catgagaagt cgatctacaa gatgccgctc ggcaagctga tgtgctgggt
    29341 atgtatggcg ttctttgtgt tcgtgatcgt gctgctgaca ctggaagatg acactcgcca
    29401 ggcgctgctg gttaccccgc tgtggtttat cgcgctgggg ttgggctggc tgtttattgg
    29461 taagaagcgg gctgctgaac tgcggaaata accgcattat catgctggat ggcgcaatgc
    29521 catccagctt ttagatcact cacccgctag ggcgcgtggg aacagtacat tgttttccag
    29581 actgatgtga tccatcaggt catcaatcag ttcattaatg ccgttataca tcgctttcca
    29641 ggtggtgcag gcttctggcg gcggtgtgac gttattggta gtgtgtttaa tcacttccag
    29701 cagttcgccc gcttcatcgt gctcgctttc cattacgctg attggcccca ttgcctggct
    29761 gcccatgcct tgtttgatca tcgggaagag gatctgctct tctttcatca tgtggctgga
    29821 aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacgc tcggtttgtc
    29881 ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagtt gctcgcggtg
    29941 acgatcgtgg tagcgcacga tgatatggtc aatgatttct gccagcgggg cgctacgcca
    30001 gtctttctca atcggttgtt cagcaagctt tgccagttca gcttcaatga cctcaacatc
    30061 cagttcttta cgtgccgccg cgcgcgccag cgtttgctta ccgccacagc agtaatccat
    30121 atcatattta cgaaacagag ctgaagcgcg aggaatagag agcgccagtt cacctaaagg
    30181 ttggtcgcga taagccatag ctgatacctc attcttaaaa agataagatg tattttaaat
    30241 gcatctttaa ggcaaaagct ataaccctta cgtagcagag gcttatttaa ctcactgcca
    30301 gcgtgtcaac tttctgagac agattcagtt aaaggctttt ctggttttgc tctgaccgcg
    30361 atgaccacgc caatcactaa cagcgcgata ccgctcagcg tcattagcgg cggcatttgc
    30421 tggcggagta aaaaggtgta cagcaaacct gccagcgttt cgaaaacaat cagcggcccg
    30481 agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc aacccatgag
    30541 caaagcacgg ctatcgcaat catcagacta ataaacacca gcggacgggg gccaaaaggt
    30601 aaggagaagt ccggcgtttg tatattcagc cagtaacagg cgacgagata gccgatgaga
    30661 gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga
    30721 ttttcccgca gccagcgggc gttgcgcagg gcataccatg cccagcaaac cacggaaact
    30781 aacgccagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag tccgtggttt
    30841 aactcagcaa tattcacaca cgccaggccg atgccaatac aaatcagtgc cggggcgagt
    30901 tttccccatg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa aacgggaatc
    30961 accaccggca gggtgccgat aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca
    31021 ctggcaaggc agaagtaata aatgaggttg cccatcatag tgagcatcaa ggcggtaagc
    31081 cagtcccgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat gggtagcgca
    31141 attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta ttccggcacg
    31201 atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc gtacagcacg
    31261 ccgctaatca ttactggcat ccattggtct gtcagaagaa acgttcagga agcagacatg
    31321 attgctcccg gcatcccgtc attatcagag aggatgcggg aggatgccac tttaattaac
    31381 tagttattaa cattaaaaag atggcttacg ctttcggcta acgttgtcgt tgggcggcca
    31441 atcaatttgc taagcgtttt gctgtcatca aacagaccgc ctttcgatgc gccaacgtca
    31501 gaatccgcca gcatatccgc cagtccgtcg ggcagtccga cgcttttcag tgccgcggcg
    31561 aaatcggctt cgctcagatt ttgataggta acctgtttgc cgctctgttt ggtcagctct
    31621 gccgctaact gtgtcaacgt ccaggcacta tcgcccgcca gttcgtaaac cttgccttcg
    31681 tgaccggctt cgctaatcac gcgtgccgca gctgccgcat aatctgcccg cgttgctgag
    31741 gcgattttgc catcgcccgc cgcaccgata aatacgccgt gttccagtgc tgccggggcg
    31801 ctggcgaggt agttttcgct gtaccagccg ttgcgcagca gggtgtaaac gatgccagaa
    31861 tcagccaaca ttttctccgt ctcgatgtgc tcatcggcga ggccgagcgg ggaggtatct
    31921 gcatgtagca ggctggtata agcgataaat ttcacaccag ccgcctttgc ggcattaata
    31981 acattacgat gctgcggggc acgttgaccc acttcgctgg aagagatcag cagtagtttt
    32041 tccactccct gaagtgcaga tgtcagtgcg gcttcatcgc cgtagtcagc ctgacgcacg
    32101 gtaatgcctt gtgctgccag agcctgggct tttgccggat tacgaacgat agccactatt
    32161 tggctgacag gaaccgtttt cttcaaggat tcaataacat agtgaccaag ttggccagtg
    32221 gcaccagtaa tagcgatcat gggaagtctc catcgttttg tcttgtgttt aaatacgcta
    32281 acacctaaac ttacttttag taagtacgca caaaaaggta agtatgaaat gagtcaggtt
    32341 agcctgtcgc aacaactgaa agagggtaat ctctttgcgg aacagtgccc gtcgcgcgag
    32401 gtgttgaaac acgtcaccag ccgttggggg gtgttgattc tggtggcgct acgcgaaggt
    32461 actcatcgct ttagcgacct gcggcgcaaa atcggcgggg tgagcgaaaa gatgcttgcg
    32521 cagtcgttac aggcgttaga acaggatggt tttcttaacc gtatcgcgta tccggtggtg
    32581 ccgccgcatg tggaatatag cctcacgccg ctgggcgagc aggtgagcga aaaggttgct
    32641 gcactggcgg actggattga gttgaatttg cccgaggtgt tggcggtgcg ggatgaacgt
    32701 gcggcataac tgccggatgc gctgagctta tctggtctac ggtgcatggc ttgtagggcg
    32761 gataagatgc gccagcatcg catccgccaa taaatgccgg atgcggttga tgatttatag
    32821 gccgaaaaag aagtgtttta cttactcaaa tccacctgat aaatcgcaaa cccgatatca
    32881 tcggtcgcga ctttattcat cggatattgc cctttctctt taataaacgc tgcggcttta
    32941 tctgacgggg aagtttcgaa acggatatcc agtttcttat cgccagcaat cggtgctaaa
    33001 cgccagttgt tatctgccgc cgggtgaatt tcccccgcac gcttcgactc atcagcaatc
    33061 cacgctgcca gcaccgagcg gttctcatcc ggtgaagcaa aagcgatatg gctgtcgccc
    33121 gtaccggcaa atttgccacc gtaagcgcga tagttattgg tggcaactag gaacatagcg
    33181 ttcggatcaa tcggcttgcc attaaaggtc aggttcttaa tccgctctgc attggcatta
    33241 atcatctggc actcgccgtc ataacgggcg ggttgggtaa catcaatctg ataattcaca
    33301 ccatcaataa catcaaagtt ataagtgcgg aaaccatccc agttgatgag tgactgtggt
    33361 ttcgtgctgt taggatcaat ctggttaaac tgtccggcgg agcactccag ccactctttc
    33421 acctctttac cgctggcttt caccacaatc agtgtattgg ggtagagata aagatcggcg
    33481 gcattacgga aggtcaactg gcctttttcc acctccacat agcttgccgg gtcatttttg
    33541 cgaccaccaa ctttaaacgg tgcggcagct gaaagcaccg gcagttttgc cagatccgga
    33601 tcgccctgaa tgtaatgttc gacatacgct ttttgcgcgt tgttcactac ttgcacggtc
    33661 ggatcgtcct gcaccagcgc cagatagcta tacatattgt cggcagattt accgattggc
    33721 ttgctgacga actggcgtgt ggcatcgtga tcggctttga gtgtttctac cagcttgctg
    33781 tcttccgccg cgagggattt tttattggcg atgtcgtaaa tcggccgagc ttccgctttc
    33841 gcctgcgtca cctgccattt accgctgtca ttactgagtt gtaagtcgac cacaccaaga
    33901 tgatcgcccc acatgcctgg cattaccgcc ggaacaccat tcagcgtgcc tttcgcgata
    33961 tcagctcctt cgatatcagc aaaatcttta cttgggaaaa cggcgtgagc atggccaaac
    34021 ataatggcgt caacacccgg aatttcactg agataataaa ctgagttttc cgccatcact
    34081 ttatacggat cggcagacag cccggaatgc gccagaacga caacgagatc ggcacctttc
    34141 tcgcgcattt caggcacgta tttgcgcacg gtttcggtaa tatcattcac cgtcactttc
    34201 ccggataaat tagctttatc ccagcccatg atttgcggcg gtacgacgcc aatatagcca
    34261 atcttcagcg tctgtttttt tccgtcttta tcgaccactt cggtgtcttt aattaaatac
    34321 ggtgtaaaca ttggctgttt ggttctggcg tcaatgacgt tggcatttac ataagggaat
    34381 ttcgctcccg ccagcgcatt tttcaggtaa tccagaccgt agttaaactc atggttgcca
    34441 agcgtgccga cggtatagtc cagcgtattt aatgccttat acactgggtg aatatcacct
    34501 gcttttaatc ctttcgccga catgtaatct gccagcggac tcccctgaat caaatcgccg
    34561 ttatcgacca gtacgctgtt tttcacttca ttgcgggcat cgttaatcag gcttgccgta
    34621 cgtaccagtc cgaatttttc cgtggcggtg tctttgtaat aatcgaaatc catcatgttg
    34681 ctatgcagat cagtggtttc catgatacgc agatcgaccg tcgctgcatt cacactggcg
    34741 gcaatcagcg tggccaggag cgttgcgcta aacttaatca tcagggacat ccttttatca
    34801 tcgggaatac gaaagaaaag ggagaataaa cgtcttactt atagaacagt gaagaatgcc
    34861 acaattttac gctttgaaaa tgatgacact atcacagttg gcgcattcat taacgatagg
    34921 gtataagtaa aacaataagt taacaccgct cacagagacg aggtggagaa atgttagatc
    34981 aagtatgcca gcttgcacgg aatgcaggcg atgccattat gcaggtctac gacgggacga
    35041 aaccgatgga cgtcgtcagc aaagcggaca attctccggt aacggcagcg gatattgccg
    35101 cacacaccgt tatcatggac ggtttacgta cgctgacacc ggatattccg gtcctttctg
    35161 aagaagatcc tcccggttgg gaagtccgtc agcactggca gcgttactgg ctggtagacc
    35221 cgctggatgg tactaaagag tttattaaac gcaatggcga attcaccgtt aacattgcgc
    35281 tcattgacca tggcaaaccg attttaggtg tggtgtatgc gccggtaatg aacgtaatgt
    35341 acagcgcggc agaaggcaag gcctggaaag aagagtgcgg tgtgcgcaag cagattcagg
    35401 tccgcgatgc gcgcccgccg ctggtggtta tcagccgttc ccatgcagat gcggagctga
    35461 aagagtatct gcaacagctt ggcgaacatc agaccacgtc catcggctct tcgctgaaat
    35521 tctgcctggt ggcggaagga caggcgcagc tgtacccgcg cttcggacca acgaatattt
    35581 gggacaccgc cgctggacat gctgtggccg cagctgccgg agcgcacgtt cacgactggc
    35641 agggtaaacc gctggattac actccgcgcg agtcgttcct gaatcctggg ttcagagtgt
    35701 ctatttatta attaatttat gcagcaggtc catcacttgc tgcacctctt cctgagtaag
    35761 cgccccgtct ttggcccatt gcacgcgacc gtctttatcc agcaccacta cagcggaact
    35821 tttttcatcc agctgccagg caccgcgtgc gacgccattg ctatcaacaa taaactgcga
    35881 ccagggataa agctttttat tactctccag actgctgcgc acaaacatgc cggaacccgg
    35941 aattgcgtcg tcggtgttaa caatggtggt ggtctggtaa cgatcgtgcg gtaacttcgc
    36001 tgatttaatc gcttcaatca gcgtcgcgtt tttctctttt gcagaggtgc gaccagcaat
    36061 atgttgcagt actcgcactt ttcccactaa ctgcgcgctg ttccaggttt tgtagctaaa
    36121 ctgatcttta tcaagcacca actcgccacg atcggtgatg ccaatcggcg gcactcgctg
    36181 accggtttcg aactgatgtg cggaagccat catcggcaac agcaggcagg tgagtgccag
    36241 aatcttgcgt agggtcatgg tgtttccttc ttatgatatg caggtgatcc gaccacttgg
    36301 gtctgagttt taatcatatg tgctatttat cgaatttccc gcaagtgtga tgccagtttg
    36361 cggtcaagcg cacaaatcat atgaaaaatg aatgcttata ctgaagaccg cgcttcggta
    36421 aaaagataat tctgaataat tgtaaccttt aggtaaaaaa agttatacgc ggtggaaaca
    36481 ttgcccggat agtctatagt cactaggcat taaaatttgc gcctcataat agttgggccg
    36541 attgtggcac cgcacaggcg taatactcag caggagataa caatgaaaat tttccaacgg
    36601 tacaacccac ttcaggtggc gaagtacgta aagatcctgt tccgtggacg gttatacatc
    36661 aaggacgttg gcgcttttga atttgataag ggtaagattc ttatcccaaa agtgaaggat
    36721 aagctgcatt tgtcagtgat gtccgaagtt aaccgtcagg ttatgcgtct gcaaacagag
    36781 atggcttaac caaagtgcta tgcagtaata agacggctcc tgattcagga gccgttgatg
    36841 tttctggggg ttacgcgacg ctttcttctt tatctttagc gtcaggcagt tttggcgaaa
    36901 gggcggtggc cttgctgtcg atccgggtca ccagcagctg gtcgatgcgg tagttatcga
    36961 tatccaccac ttcaaatttg tagccggcga atttcaccga atcggtgcgt ttcgggatct
    37021 tacgcagcat aaacatcata aagcctccga tggtttcgta gttgcccgac tgcgggaact
    37081 cgtcaatatc cagcacgcgc atgacgtcgt caattggggt gccgccgtca atcagccatg
    37141 agttctcatc acgggcgaca atctgctctt ccagcccctg accgaccaga tcgcccatca
    37201 gcgtggtcat cacgtcgttg agggtgatga tccccaccac cagcgcgtac tcgttcatga
    37261 tcaccgcgaa gtcttcacct gcggttttaa aactttccaa cgcctctgaa agggttaacg
    37321 tatccggcac aatcagcgtg ttgcgaattt gtacgccgct gttcagtgcc aggctttggt
    37381 tagccagcac gcggttcagc aggtctttag aatcgacata accgatgatg tggtcaatat
    37441 cttcattaca gacgaggaac ttagagtgcg gatgttccgc caccttattc ttcaggcttt
    37501 gctcatcttc gtggagatca aaccaaatca cgttttcacg cggtgtcatt gaagacggaa
    37561 cggtacggga ttccagctca aagacgtttt caatcagctc gtgttcctgt ttacgtaaca
    37621 cgcccgccag cgcaccggct tccactaccg cgtagatgtc atcagaagtg atgtcatctt
    37681 tacgtaccat tggcagtttg aaaatacgga agatcatgtt cgccaggccg ttgaagaacc
    37741 acaccagcgg ggtgcaaacg tacaggcaga agcgcatcgg gttgatgata cgcaaagcca
    37801 ccgcttctgg cgcaatcata ccgatgcgtt tcggggttaa atccgcaaac aggataaaca
    37861 tgccagtcac taacgagaaa gagagaataa agctcagttg ctcagagagt tctgccgaca
    37921 tatagcggga gaacaggctg tgaaaagctg gagaaaatgc cgcatcaccg acgataccgc
    37981 cgagaatcgc cactgcgttc agaccgattt ggaccacggt aaagaacatg ccgggatttt
    38041 cctgcatatt cagaacgcgt tgggcattta tattgccttc atcagccagc agtttaagtt
    38101 tgattttgcg tgaggcggca agcgagatct cggacatcga gaagaacgca cttacagcga
    38161 tcaaacagag tatgactaaa atactgttta acatatctta tccgaccttt caggccagat
    38221 cctcggaagg ggaagttgat taatttgtgt gaaatacaca ttgaaagccg attcgaagaa
    38281 agtgaaccgg cattttcagt ggctagtata gcgtaaggta ctgtaaagtc gccagagggt
    38341 taaaattcag actgctgata aatgcaatac tgcctgatgc gctatgccaa tcaggcctac
    38401 gcagtccttg caatatattg aatttggacg attttgtagg cctggtaagg cgtttacgcc
    38461 gcatctggca taaataaagc gcactttatc aacaatctac atttgtaccc gtaacgctat
    38521 gcttccggtg gcagacagac gccaattccg ccaattccac agtaaccata cgggttttta
    38581 tgcagatatt gctggtggtc atcttcggca taataaaacg gtgtggcgtt agcgatttcc
    38641 gtggtgatgt gacgatcatc atcggcggca agcatcgccg cctgaaaacg ttccagactg
    38701 gcgcgagctg cggcatcctg ttctggggtc agcggataaa tcgctgaacg atactgcgtg
    38761 ccgtggtcat tgccctgacg cattccctgg gcgggatcgt gattctccca aaatacctgt
    38821 agcaactgct catagctgat gacggaagga tcgtaaacaa tgcgtaccgc ttcggcatga
    38881 cccgtatcac cggagcacac ttcccgataa gtcggatttg gcgtatagcc gccggtatag
    38941 cctgcggcgg tgctgtaaac gccgggtaac tgccagaaca gacgctccac accccagaaa
    39001 caacccatcg caaaaatggc aatctccatt ccgtcaggta cattggtcat tgagtgaccg
    39061 ttgaccgcat gcagcgtggc tacgggcatc ggggtgttac gtccaggcag ggcatcggcg
    39121 ggggaaacca gatgcttttt atcaaataaa ctcatggtgt cgctctcccg aaaatcggtc
    39181 attggggtta aggttgtaac aagaggcgta tttgcacaca ataaccaccg tgaataggtc
    39241 taaagtaaaa cataagaaat atttggggtt tagtctgctt tttaatccat attactggat
    39301 ttttgttaag ccgtttaacg gcgttccagg ggcaggaaaa aaggatattc aggagaaaat
    39361 gtgcgctata tccgacagtt atgctgtgta agcttactct gcttaagcgg atctgccgtc
    39421 gccgcgaacg tccgtctaca ggtcgagggg ttatcgggac agctggaaaa gaacgttcgt
    39481 gcgcagcttt ctacgattga aagtgatgaa gtgacgccag accgtcgctt tcgcgcacgc
    39541 gtcgatgatg ccatccgtga aggtctgaaa gcgctgggtt attaccagcc gaccattgaa
    39601 tttgatctcc gtccaccgcc aaagaaaggg cggcaggtat tgatcgccaa agtcacgcca
    39661 ggtgtgccgg tgttaattgg cggcaccgat gtggtattgc gcggcggcgc gcggaccgat
    39721 aaagactatt tgaaattgct cgatactcgc ccggctattg gcacggtgct gaaccagggc
    39781 gattatgaaa atttcaaaaa gtccttaacc agcattgcgt tgcgtaaagg ttatttcgat
    39841 agcgaattta ccaaagcgca gctgggcatt gcgctcggcc tgcataaagc cttctgggat
    39901 attgattata acagtggcga acgttaccgc tttgggcatg tgacctttga aggatcacaa
    39961 atccgcgatg aatacctgca aaatctggtg ccgtttaaag agggcgatga gtacgaatcg
    40021 aaagatctgg cggaactgaa ccgccgactt tctgctaccg gctggtttaa ctcggtggtg
    40081 gtggctccac aatttgataa agcgcgcgaa acgaaagtat tacccttgac gggcgtggtt
    40141 tcgccgcgaa ctgaaaacac catcgaaacc ggggtcggtt actctacgga cgtgggaccg
    40201 cgcgtgaaag cgacgtggaa aaagccgtgg atgaactctt atggtcacag tctgaccacc
    40261 agtaccagta tttccgcgcc ggaacagacc ctcgacttca gctataaaat gccgctgctg
    40321 aagaatccac tggaacaata ttatttggtg cagggcggtt ttaagcgcac tgacctgaac
    40381 gataccgaat ctgactccac tacgctggtg gcttctcgct actgggatct ctccagcggc
    40441 tggcagcgtg ccattaacct gcgctggagc ctcgaccact ttacccaggg tgaaattacc
    40501 aacaccacga tgctgtttta tcctggggtg atgattagcc gcacgcgttc tcgtggtggc
    40561 ctgatgccaa cctggggcga ctcgcaacgc tactctatcg actactccaa cacggcctgg
    40621 ggctcagatg tcgatttctc cgttttccag gcgcagaacg tctggatccg cacactgtac
    40681 gatcgccatc gttttgtgac acgcggcacg ctgggctgga ttgaaacggg tgatttcgac
    40741 aaagtaccgc cggatctgcg tttcttcgcc gggggcgatc gcagtattcg cggctacaaa
    40801 tacaaatcta tcgccccgaa atacgccaac ggtgacctga aaggggcctc gaagctgata
    40861 accggatcgc tggaatacca gtacaacgtg accggaaaat ggtggggcgc ggtgtttgtc
    40921 gatagtggcg aagcggtaag cgatattcgc cgcagcgact ttaaaaccgg taccggggtt
    40981 ggcgtgcgct gggaatcgcc ggtcgggcca atcaaactcg attttgccgt accggtcgcg
    41041 gataaagacg aacacgggtt acagttttac atcggtctgg ggccagaatt atgagtttat
    41101 ggaaaaaaat cagcctcggc gtggttatcg ttatcttact gttgctggga tcggtggcgt
    41161 ttctggtggg caccaccagc ggcctgcatc ttgtatttaa agcggcggat cgctgggtgc
    41221 caggactgga tattggcaag gtcaccggcg gctggcgcga tctcaccttg tctgacgttc
    41281 gttatgagca gccaggcgtg gcggtaaaag cgggtaatct gcatctggcg gtcgggcttg
    41341 agtgcctgtg gaacagtagc gtttgtatta atgacctggc gctgaaagac attcaggtca
    41401 acatcgacag taaaaaaatg cctccttctg aacaggttga agaagaggaa gatagcggtc
    41461 cgctggatct ctccacgccg tatcccatca ccctgacacg ggtggcgctg gataacgtca
    41521 acatcaagat tgatgacacc acggtatcgg tgatggactt cacctccggc ctgaactggc
    41581 aggagaaaac cctgaccctg aaaccgacgt cgctgaaagg cctgttgatt gccctgccga
    41641 aagtggcgga agtggcgcag gaagaagtgg tcgaaccgaa aattgaaaat ccgcagccgg
    41701 atgaaaagcc gctcggcgaa acgttgaaag atctcttttc tcgtccggta ttgccggaaa
    41761 tgaccgacgt gcatttgccg cttaacctga acattgaaga gtttaagggc gagcagctgc
    41821 gcgtgacggg cgacacggac atcaccgtga acaccatgct gctgaaagta agcagcatcg
    41881 acggtaatac taaactggac gccctggata tcgattccaa tcaagggatc gtcaacgcca
    41941 gcggcacggc gcagctgtca gacaactggc cggtggatat caccctcaac agcacactga
    42001 acgtggagcc gttgaaaggt gaaaaagtga agctaaaagt gggcggcgcg ctgcgcgaac
    42061 agctggagat tggcgttaat ctttccggtc cggtggatat ggatttacgc gcccagacgc
    42121 gactggcgga agccggattg ccgctcaacg tggaagtgaa cagcaaacag atttactggc
    42181 cgttcactgg tgagaagcag tatcaggcgg atgatctgaa actgaaactt accggcaaaa
    42241 tgaccgatta cacgctctct atgcgtacgg cagtgaaggg acaggagatc ccgcccgcga
    42301 ctattaccct cgacgcgaaa ggtaatgaac agcaggtcaa tctcgacaaa ctcaccgttg
    42361 cggcgctgga aggcaaaact gaactcaagg cgttgctcga ctggcaacag gccattagtt
    42421 ggcgcggtga gctaacgctt aatggcatta acaccgccaa agagttcccg gagtggccgt
    42481 cgaaactcaa tggcttgatt aaaacccgcg gtagcctgta cggcggcacc tggcagatgg
    42541 aggtgccaga actgaagctg accggtaacg tcaaacagaa caaagtgaac gttgacggca
    42601 cgctgaaagg caacagttat atgcagtgga tgatcccagg gcttcatctg gaactggggc
    42661 caaacagtgc cgaagtgaaa ggcgagctgg gggtaaaaga tctcaatctt gatgccacca
    42721 tcaacgcgcc ggggctggat aacgcgctgc cggggcttgg cggtacagcg aaagggctgg
    42781 tgaaagtacg cggcacggtg gaagcgccac aactactggc agatatcacc gcgcgcggcc
    42841 tgcgctggca ggaactttcc gtggcgcagg ttcgcgtgga aggcgacatt aaatccaccg
    42901 atcagatcgc cgggaaactc gacgtacgcg ttgagcaaat ttcgcagccg gatgtaaata
    42961 tcaacctcgt caccctgaat gccaaaggca gcgaaaagca gcacgagcta cagttgcgga
    43021 ttcagggcga gcctgtctcc gggcagctta atctggcagg aagttttgat cgcaaagaag
    43081 aacgctggaa gggaactctt agcaataccc gcttccagac gccggtcggc ccgtggtcgc
    43141 tgacccgcga tattgcgctg gattaccgca ataaggagca aaaaatcagc atcgggccac
    43201 actgttggct taacccgaat gcggagctgt gcgtgccgca aactatcgat gcgggggccg
    43261 aagggcgtgc ggtggtgaat ctcaaccgct tcgacctcgc catgctgaaa ccgtttatgc
    43321 cagaaaccac tcaggccagc ggtatcttca cgggtaaagc ggatgttgcc tgggacacca
    43381 cgaaagaggg gctgccgcag ggcagtatca ccctttcggg gcgtaacgtg caggtaacgc
    43441 aaaccgtcaa cgatgcggcg ctgcccgtgg cgtttcagac gctgaatctg acggcggaat
    43501 tgcgtaacaa ccgtgccgaa ttgggctgga ccatccgcct gaccaataac ggccagtttg
    43561 atggacaggt gcaggtgacc gatccgcaag gccgccgtaa tcttggtggc aacgtcaata
    43621 tccgtaactt caaccttgcg atgataaacc ccatctttac ccgtggggaa aaagcagcgg
    43681 ggatggtgag tgccaacttg cgtctgggtg gtgatgtgca aagcccgcag ttgtttggtc
    43741 agcttcaggt tacgggtgtg gatatcgacg gcaactttat gccgtttgat atgcaaccga
    43801 gccagcttgc ggtcaacttt aacggtatgc gctcgacgct tgccggtaca gtacggaccc
    43861 agcagggtga aatctacctg aacggtgatg ccgactggag gcaaattgaa aactggcggg
    43921 cgcgggtaac ggcgaaaggc agtaaagtgc ggatcaccgt gccgccgatg gtacgaatgg
    43981 atgtatcgcc agatgttgta ttcgaggcta caccaaacct gtttaccctc gatggtcgcg
    44041 tggatgtccc ttgggcgcgc atcgtggtgc acgatctgcc ggaaagcgca gtaggcgtct
    44101 ccagcgatgt ggtgatgctt aacgataacc tgcaaccgga agagccgaaa acggcgtcga
    44161 ttccgattaa cagtaacctg attgtccacg ttggcaataa tgtgcgcatt gacgcctttg
    44221 gcctgaaagc gcggctgacg ggcgatctta acgtcgttca ggacaaacaa gggctgggcc
    44281 tgaacgggca gatcaacatc cctgaagggc gcttccatgc ctatggtcag gatctgattg
    44341 tgcgtaaggg tgagttactg ttctctggtc cgccggatca accgtatctt aatattgaag
    44401 ctattcgtaa cccggatgct acagaagacg acgtaatcgc cggagttcgc gtcactggtc
    44461 tggcggacga accgaaagcg gagatcttct ctgacccggc gatgtcgcaa caagctgcat
    44521 tgtcttattt gctacgtgga caagggctgg agagcgatca gagcgacagt gcggcaatga
    44581 cctcgatgct gattggtctg ggggttgcgc aaagtggcca gattgtgggt aaaatcggcg
    44641 agacgtttgg cgtaagcaat ttagcgctcg acacccaggg agtaggcgac tcctcccagg
    44701 tagtggtcag cggctatgta ttgccaggtc tgcaagtgaa atatggcgtg ggtatatttg
    44761 actctatagc aacactcacg ttacgttatc gcctgatgcc taagctatat ctggaagccg
    44821 tgtctggtgt agaccaggca ctggatttgc tctatcagtt cgagttttag caatgcgaat
    44881 atttgtctac ggcagtttac gccacaaaca aggcaacagt cactggatga ccaatgccca
    44941 gttactgggc gatttcagta tcgataacta ccagttgtat agcctgggcc actatccagg
    45001 cgcagttccg gggaacggaa cagtacacgg tgaagtttat cgtattgaca acgccacgct
    45061 ggccgaactt gatgccttgc gcaccagggg cggtgaatac gcgcgccagt tgattcagac
    45121 gccgtacggg agtgcatgga tgtacgttta tcaacgaccc gtcgatggat taaagctaat
    45181 tgaaagcggc gactggttag acagggataa gtaaccatat gcatacgcca ccttcgggtg
    45241 gcgttgtttt ttgcgagacg actcgcattc tgttttgtaa ttccctcacc ctttgctttt
    45301 ctctccgagc cgctttccat atctgttaac gcataaaaaa ctctgctggc gttcacaaat
    45361 gcgcaggggt aaaacgtttc ctgtagcacc gtgagttata ctttgtataa cttaaggagg
    45421 tgcagatgcg tattaccata aaaagatggg ggaacagtgc aggtatggtc attcccaata
    45481 tcgtaatgaa agaacttaac ttacagccgg ggcagagcgt ggaagcgcag gtgagcaaca
    45541 accaactgat tctgacaccc atctccaggc gctactcgct tgatgaactg ctggcacagt
    45601 gtgacatgaa cgccgcggaa cttagcgagc aggatgtctg gggtaaatcc acccctgcgg
    45661 gtgacgaaat atggtaaaga aaagtgaatt tgaacgggga gacattgtgc tggttggctt
    45721 tgatccagca agcggccatg aacagcaagg tgctggtcga cctgcgcttg tgctctccgt
    45781 tcaagccttt aatcaactgg gaatgacgct ggtggccccc attacgcagg gcggaaattt
    45841 tgcccgttat gccggattta gcgttccttt acattgcgaa gaaggcgatg tgcacggcgt
    45901 ggtgctggtg aatcaggtgc ggatgatgga tctacgcgcc cggctggcaa agcgtattgg
    45961 tctggctgcg gatgaggtgg tggaagaggc gttattacgc ttgcaggcgg tggtggaata
    46021 aggtgtgttt atttatcgct ggcataaaaa aacccttact aaccgaagct cggcgttcag
    46081 ggttattgcg ctagaagaac ttatttattc tttgcgcgct cgaaggaagc aacgatttca
    46141 gctttagcgg cttctgcgtt ttcccaacct tcaactttca cccacttgcc tttttcgagg
    46201 tctttgtagt gctcgaagaa gtgagcgatc tgcgctttca gcagctctgg cagatcgtta
    46261 acgtctttaa tgtgatcgta ttctttgctc agcttgctgt gcggaaccgc aaccagtttc
    46321 gcatcttcac cggcttcgtc ggtcattttc agaacgccaa ccggacggca acggatcaca
    46381 gaacccggct gcagcgggta cggagtcggg accagtacgt caaccgggtc accgtccaga
    46441 gacagggtgt ggttgatgta accgtagttg cacggataga acatcgcggt ggacatgaag
    46501 cggtcaacga acagtgcgcc gctctctttg tcgatttcgt atttgatcgg atctgcgtta
    46561 gccgggatct caataacaac gtagatgtct tccggcagat ctttacccgc agggacgttg
    46621 agtaagctca tgtctgtttc ctttaaaaat atgtggcaaa taagtgccga gtattatagc
    46681 caactcgcgc cgaatgtctt cgcttgtttt cgtcttcatt ttcctttttc gccagtttta
    46741 agacggtata cagagcagga aaatccataa ccgtagccgc atttttcata gtgagatgaa
    46801 agcgattaca aacttgtgat taacgtttta tttactttta tgaagtgtga tgtaacgcaa
    46861 tccgttacat aacgaattgt ctatagtttt tccgcgaaca tcttttcacc aataataatt
    46921 accccgacga ggacaaccct atgtggaaac gcttacttgt agtctctgca gtctcggcag
    46981 ccatgtcgtc tatggcgttg gccgctccat taaccgtagg attttcgcag gtcggatcgg
    47041 aatccggctg gcgcgctgca gaaaccaatg tggcgaaaag tgaggccgaa aaacgcggaa
    47101 ttacgctgaa aattgccgat ggtcagcaaa agcaggaaaa ccagattaaa gcggtacgtt
    47161 ccttcgtcgc gcaaggggtg gatgcgatct ttatcgctcc ggtggtcgcg acaggttggg
    47221 aaccggtatt aaaagaggcg aaagatgccg aaatcccggt cttcttgctc gaccgttcca
    47281 tcgatgtgaa agacaaatct ctctatatga ccaccgttac tgccgacaac atcctcgaag
    47341 gtaagttgat cggtgactgg ctggtaaaag aagtgaatgg caaaccatgc aacgtggtgg
    47401 agctgcaggg caccgttggg gccagcgtcg ccattgaccg caagaaaggc tttgccgaag
    47461 ccattaagaa tgcgccaaat atcaaaatta tccgctcgca gtcaggtgac tttacccgca
    47521 gtaaaggcaa agaagtcatg gaaagcttta tcaaagcgga aaacaacggc aaaaacatct
    47581 gcatggttta cgcccataac gatgacatgg tgattggcgc aattcaggca attaaagaag
    47641 cgggcctgaa accgggcaaa gatatcctca cgggttccat tgacggtgta ccggacatct
    47701 acaaagcgat gattgatggc gaagcgaacg ccagtgttga actgacgccg aatatggcgg
    47761 gacccgcttt tgacgcgctg gagaaataca aaaaagacgg caccatgcct gaaaagctga
    47821 cgttaaccaa atccaccctt tacctgcctg ataccgcaaa agaagagtta gagaagaaga
    47881 aaaatatggg gtattgaggg ttgctatgcc tgatgccgat tcgtaggccg gataaggcgc
    47941 tcgcgccgca tccggcggtg gtgcgctgaa gcctgatgcg acgcttaccg cgtcttatca
    48001 tgcctacgag ttcggtgctc tgtgtaggcc ggataaggcg ttcacgccgc atccggcagt
    48061 ggtgcaacac cggacttaac accgtgcaca atctgtcccc tcgccccttc ggggagaggg
    48121 ttagggtgag ggggacaaac ggcgcagctc ttaacatcag gcacgatcta ttcccagact
    48181 tttagagcgt ggctaagggg gaaaccatga cgaccgacca acaccaggaa atcctccgca
    48241 ccgaaggatt aagtaaattt ttccccggcg tcaaagcgtt agacaacgtt gatttcagcc
    48301 tgcgccgtgg cgaaatcatg gcgctgctcg gtgaaaacgg ggcgggaaaa tcgacgctaa
    48361 tcaaagcatt aaccggtgta taccacgccg atcgcggcac catctggctg gaaggccagg
    48421 ctatctcacc gaaaaatacc gcccatgcac aacaactcgg catcggaacc gtttatcagg
    48481 aagtcaacct gctacccaat atgtcggtcg ctgataatct attcataggt cgcgaaccca
    48541 aacgtttcgg ctttctacgc cgcaaagaga tggaaaagcg tgccaccgaa ctgatgacat
    48601 cttacggttt ctccctcgac gtgcgtgaac cgcttaaccg cttttcagtc gcgatgcagc
    48661 aaatcgtcgc catttgccgg gctatcgatc tctctgccaa agtgctgatc ctcgatgaac
    48721 ccaccgccag tctcgacact caggaagtag aattactgtt tggcctgatg cgtcagttgc
    48781 gcgatcgcgg cgtcagcctg atctttgtca ctcactttct cgatcaggtc tatcaggtca
    48841 gcgatcggat caccgtttta cgcaacggca gtttcgtagg ctgtcgggaa acgcgcgagc
    48901 taccgcagat cgaactggta aaaatgatgc tggggcgcga gctggatacc cacgcgctac
    48961 agcgtgccgg gcgaacattg ttgagcgaca aacccgttgc cgcgttcaaa aattacggca
    49021 aaaaaggaac gatagcaccg tttgatctcg aagtgcgccc cggcgagatc gtcggcctgg
    49081 caggcttgct gggatccgga cgtaccgaaa ccgccgaagt gatcttcggt atcaaacctg
    49141 ctgacagcgg cacggcgttg atcaaaggca aaccgcaaaa cctgcgatcg ccacatcagg
    49201 cttcggtact gggcatcggc ttctgcccgg aagacaggaa aaccgatggc atcatcgctg
    49261 ccgcctcggt gcgggaaaat atcatcctcg ctctccaggc ccagcgcggc tggctacgac
    49321 ccatttcccg caaagagcag caagagattg ccgaacgctt tatccgccag cttggcattc
    49381 gcacaccttc aactgaacaa ccgattgaat ttctctccgg cggcaatcag caaaaagtgt
    49441 tgctttcacg ttggctactg acccgaccgc aatttctgat cctcgatgag ccaacccgtg
    49501 gcattgatgt tggtgcccac gccgagatca tccgcctgat tgaaacgcta tgcgccgatg
    49561 gtctggcgct gctggtgatc tcctccgaac tggaagagct ggtgggctat gccgaccggg
    49621 tgatcatcat gcgcgatcgc aaacaggtgg cggagatccc gctggcagag ctttccgttc
    49681 cggcgatcat gaacgccatt gcggcgtaag gagaacagtg tgatgcctca atctcttccg
    49741 gacaccacta cgccaaaaag gcgctttcgc tggccgacgg ggatgccgca gctggtagca
    49801 ctattgctgg tgctgctggt cgacagcctg gtagccccgc atttctggca ggtagtgctt
    49861 caggatgggc gtttgttcgg tagccccata gacattctta accgtgcagc ccccgttgcg
    49921 ttactggcga ttggtatgac gctggtgatc gccaccggtg ggattgatct ctccgtaggg
    49981 gcggtgatgg ctatcgccgg agccacaacg gctgcgatga cggtcgcggg agtcagcctg
    50041 ccgattgttt tgttaagcgc cctgggcacc ggcatcctgg cgggattgtg gaacggcata
    50101 ctggtagcga tcctcaaaat tcagccattt gtcgccaccc taatcctgat ggtcgccggg
    50161 cgcggcgtgg cgcaactgat cacctccggc cagatcgtca cgtttaactc gccggatctc
    50221 tcatggtttg gcagcggatc gctgttgttc ctgccaacgc cggtcattat cgcggtgctg
    50281 acgcttctcc tgttctggct gttgacccgc aaaacggcgc tggggatgtt tatcgaagcc
    50341 gttggtatca acattcgggc ggcaaaaaat gccggggtaa acacgcgaat catcgtcatg
    50401 ctcacctacg tgttgagcgg gctatgtgcg gcgattgcgg gcattatcgt ggcggcggat
    50461 attcgcggtg ccgatgccaa caacgccgga ttatggctgg agctggacgc cattctcgcg
    50521 gtggtgattg gcggcggatc gctgatgggc gggcgcttta acctgctgct ctcagtcgtg
    50581 ggggcgctga ttattcaggg gatgaacacc ggaattttac tttctggctt tccgccggag
    50641 atgaaccagg tcgtaaaagc ggtggtggtg ctttgtgtgc tgattgttca gtcgcaacgc
    50701 tttatcagtc tgattaaagg agtacgtagc catgataaaa cgtaacttgc cgctgatgat
    50761 caccatcggc gtctttgtgc tgggctatct ctactgcctg acccagtttc ccggttttgc
    50821 ttccacaaga gtgatctgca atatcctgac cgataacgcc tttcttggga taatcgccgt
    50881 tggcatgacc tttgtgatcc tctctggcgg tatcgatctc tccgtcggtt cggtgatcgc
    50941 ctttactggc gtgtttctgg caaaagtgat tggcgatttc ggcctctcgc cgctgctggc
    51001 gtttccgcta gtgctggtga tgggctgtgc ctttggcgca tttatggggc ttttgatcga
    51061 cgccctgaag atcccggcat ttatcattac cctggcagga atgttctttt tgcgcggcgt
    51121 cagctatctc gtttcagaag agtcgatccc gataaaccat cccatttatg acacgctctc
    51181 aagccttgcg tggaaaatcc ctggcggcgg tcgcttaagt gcgatgggac tgctgatgct
    51241 ggcggtggtg gttatcggca tattcctcgc gcatcgtacc cgttttggta atcaggtata
    51301 cgccattggc ggcaacgcaa cgtcggcaaa cctgatgggg atttctaccc gcagcaccac
    51361 tattcgcatt tatatgctcg ccaccggact ggcaacgctg gcggggattg tcttctcgat
    51421 ttatacccag gccggatatg cgctggcggg cgtaggtgtg gaactggatg ctatcgcctc
    51481 agtggtaatt ggcggtacgc ttttgagcgg tggcgttgga acggtattag ggacgctttt
    51541 tggcgtggcg attcaggggc taattcagac ttatatcaac tttgacggta cgctgagttc
    51601 ctggtggacg aaaatcgcca tcggcatttt gctgttcatt tttattgcct tacagcgtgg
    51661 attaacggtg ctgtgggaga atcgtcagag ttcgccagtg acaagagtca atattgcgca
    51721 gcgataaaaa cgcctctccg tgtggagagg cgcaggagat tacgcgtccg ggaattcacg
    51781 gataaagcgt tcgacatctt caaccatatg gtcgttgccg acaaagaacg aacggcgctg
    51841 gtgcagagtt tccgggatga tatccagaat acgctctttg ccatcgctcg ctttaccgcc
    51901 cgcttgttcc gccaggaatg ccatcgggtt gcactcatac agcaaacgca gtttgccgtc
    51961 cgggtggctg gcggtgcttg ggtagagata aataccgcct ttcagcaggt tacggtggaa
    52021 atccgcgacc agtgaaccga tataacgtga ggtatacggg cggttggtgg atttatcttc
    52081 ttcctggcag aatttaatgt acttcttcac cccgttcgga aacttaatgt agtttccttc
    52141 gttgatggag taggttttgc ctttctccgg gaagcgcatc cgttcctggc acaggcagaa
    52201 aacgcccagc gaaggatcgt aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag
    52261 catggtagag gagccgtata ccacgtaacc tgccgcaacc tgtttgttac caggctggag
    52321 gaaatcttct tccgttaccg gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat
    52381 ggtaccgaca gagacgttaa catcgatgtt ggacgagcca tccagggggt ccatcagcac
    52441 cacgtatttt gcgtgttcac agccttcaaa gacgacaatc tcatcttctt cttcagaggc
    52501 aatgcccgca acgatatcgc gtgctttcag tgcggctttc agtttttcat tagcgaacaa
    52561 gtcgagtttc tgctgaacct cgccctgcac gttctcagca ccgctggcac ccaggatatc
    52621 aaccagtcct gctttgttga tatcgcgatg gataatcttg gcgcccagtt ttattgccga
    52681 cagcaaagca gtgagctcac cggtagcatg agaaaactcg tgctgctttt cgacaataaa
    52741 ttcacctaac gttttcataa aactttccct gcaatgttta tggagtaaag cgaccgcaac
    52801 aatcttaaca aataatctca atgttgcgct caggtgaatc gcgccagcaa attgcggatt
    52861 atcctgaaat gcgtttctca cttgcccgac atatgcgtaa aatgagcggc agattaaaaa
    52921 aggatagtga cgtatgcgca ttcatatttt aggaatttgt ggcacattta tgggcggtct
    52981 ggcgatgctg gcgcgccagt taggccatga agtaacgggt tcggacgcca atgtgtatcc
    53041 gccgatgagc accttacttg agaagcaagg cattgagctg attcagggtt acgatgccag
    53101 ccagctcgat ccgcagccgg atctggtgat tattggcaac gccatgaccc gtggaaatcc
    53161 gtgtgtggaa gcggtactgg aaaaaaacat cccttatatg tcaggtccac agtggctgca
    53221 cgattttgtg ctgcgcgacc gctgggtgct ggccgttgcc ggtacacacg gcaaaactac
    53281 caccgcggga atggcgacct ggattctgga acagtgcggt tacaaaccgg gatttgtgat
    53341 cggcggtgtg ccggggaact ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt
    53401 tatcgaagcg gatgagtatg actgcgcctt cttcgacaaa cgctctaaat ttgtccatta
    53461 ctgcccgcgt acgctgatcc tcaacaacct tgagttcgat cacgccgata tctttgacga
    53521 cctgaaagcg atccagaaac agttccacca tctggtgcgt atcgttccgg ggcagggccg
    53581 tattatctgg ccggaaaatg acatcaacct gaaacagacc atggcgatgg gctgctggag
    53641 cgagcaggag ctggtgggtg agcagggtca ctggcaggcg aaaaagctga ccactgacgc
    53701 ttctgaatgg gaagtcttgc tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt
    53761 gggcgaacat aatatgcaca atggcctgat ggcgattgcg gcggctcgcc atgttggtgt
    53821 agcgcctgca gatgccgcta acgcgctggg ttcgtttatt aacgctcgtc gccgtctgga
    53881 gttgcgtggt gaagcgaatg gcgtgacggt atatgacgat tttgcccatc acccgacggc
    53941 aattctggca acgcttgcgg cgctgcgtgg caaagttggt ggtacggcgc gcattattgc
    54001 tgtgctggag ccgcgctcga ataccatgaa aatggggatc tgcaaagacg atctggcacc
    54061 ttcattaggt cgtgccgatg aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt
    54121 ggcagaagtg gcagaagcct gcgttcagcc tgcacactgg agtggcgatg tggatacgct
    54181 ggcagatatg gtggtgaaaa ccgctcagcc tggcgaccat attctggtca tgagcaacgg
    54241 cggttttggt gggatccatc agaaactgct ggatggtctg gcgaagaagg cggaagctgc
    54301 gcagtaattc ggcctcagcc tgagatagca ttgccggata aggcgtttac gccgcatccg
    54361 gcatttgagc atagtgcctg atgcgacgct tgatgcgtct tatccggcct acaggtgcac
    54421 cgcatccggc aaaccatact cacatcaaca acgaaaatta cccttcattc tccgccagtt
    54481 cgcgcagata ctggaaaatc tggcgtgcgg atttcggcgg cttattccct tctttctctt
    54541 tcttcgcgtt acggatcaga gtacgcagct gctgacgatc ggcatcgggc cacagattca
    54601 atacttcagc gatggcgtca tcaccttgat cgatcagacg atcgcgcagg ttttccagtt
    54661 tatggaacag caccacctgc tggttgtgac ggtttttcag cttatccagc gcctgacgaa
    54721 taggctctac gtcgcgctgg cgcagcatct taccgatgag ctgcaactgg cggcggcgac
    54781 cttccatctt aatacgctgg gcgagctcaa tagccgcgcg cagatccgca tctaacggga
    54841 tcttatccag cgcgtttttc cccagatcaa caatttccgc gccaaggcgt tttagctcct
    54901 cggcatcacg tttaatttca cttttactga cccagataat ttcatcgtct tcgtcttcga
    54961 tgtcatcacc gggaacgtcg tcgagccagt cttcgggctg cttagtcatc tcaggctcct
    55021 taaaaaaaga ggctaatgtt accagttaag atgcgcactg aaaaacggtt ctctgttaga
    55081 cttcagagaa actctctaca ttatggcact tgcaatgaaa gtaatctctc aagttgaagc
    55141 gcagagcaag attctggaag aagcagtttc cactgcgctg gagttggcct caggcaaatc
    55201 ggacggtgcg gaagttgccg tcagcaagac caccggcatt agcgtaagca cgcgttatgg
    55261 tgaagtggag aatgttgaat tcaatagcga tggcgcgctg gggatcactg tttatcacca
    55321 gaaccgcaaa ggtagcgcat catccaccga tttaagcccg caggccattg cccgcaccgt
    55381 acaggcggcg ctggatattg cccgttatac ctcgccagat ccctgtgccg gcgtggcaga
    55441 caaagagctg ctggcctttg acgcaccaga tctcgacttg ttccaccctg cggaagtttc
    55501 cccggatgaa gccattgaac tggcggcccg cgcagaacag gcggcattgc aggcggacaa
    55561 acgcattacc aataccgaag gcggcagttt taacagccac tacggtgtca aagtttttgg
    55621 caacagccac ggcatgttgc agggttactg ctctacgcgt cattcactct ccagctgtgt
    55681 aattgccgaa gaaaatggcg atatggagcg tgattacgcc tacaccattg gccgcgcgat
    55741 gagcgatctg caaacgccag agtgggtcgg ggccgactgt gctcgccgta ctttatcgcg
    55801 cctgtcaccg cgtaaactct ccaccatgaa agcgccagtc atttttgcca atgaagtggc
    55861 aaccgggctt tttggccatc tggtgggggc gatagcgggt ggctcggttt accgtaaatc
    55921 tacctttctg ctggattcgc tgggtaaaca aattctgccg gactggctga ccattgaaga
    55981 gcatccgcat ctgctgaaag ggctggcgtc gacgccattc gacagcgaag gtgtgcgcac
    56041 cgagcgtcgc gatattatta aagatggcat cctgactcag tggctgctga ccagctactc
    56101 ggcgcggaaa ctggggctga aaagcaccgg acatgcgggc ggtattcaca actggcggat
    56161 tgccggacaa gggctaagct tcgaacaaat gctcaaagag atgggcaccg ggctggtggt
    56221 gacggagctg atgggccagg gcgtgagtgc cattaccggt gattattccc gtggtgcagc
    56281 gggcttctgg gtagagaatg gcgaaattca gtatcccgtt agtgaaatca ccatcgcagg
    56341 taatttaaaa gatatgtggc gcaatattgt caccgtcggt aacgatattg aaacacgcag
    56401 taatatacag tgtggttctg tgctgttgcc ggagatgaaa atcgccgggc agtaaaaaac
    56461 tggcgcgacc tgccgcgcta taaactaaaa aattacacaa aatcattcgc actgcatcga
    56521 gacggaaagt gaatgagggc cggggagcgt actcgcagta cgtgacccgg gcgaatgagc
    56581 gtagccgtcg atgaggcagt gtgaaggatg aagtgtaaat aaaaaaggaa gtgagcaatg
    56641 cgtaaaagcc tgttagctat tcttgcagtc tcctcgttgg tattcagttc tgcgtcgttt
    56701 gccgctgatc ttgaagacaa tatggaaacc ctcaacgaca atttaaaagt gatcgaaaaa
    56761 gcggataacg cggcgcaagt caaagacgcg ttaacgatga tgcgcgccgc agcgctggat
    56821 gcgcaaaaag caacgccgcc gaagctcgaa gataaatcac cggacagccc ggaaatgaaa
    56881 gatttccgcc acggtttcga cattctggtc ggtcagattg acgacgcgct gaagctggca
    56941 aatgaaggta aagtaaaaga agcgcaggcc gccgcagagc aactgaaaac gacccgcaac
    57001 gcctatcacc agaagtatcg ttaattcctc atttccctgt tgcctgcact caggtaacag
    57061 ggaacttatc atcgcaaatg atgcaccacc tgattgctgc tgccgcgcca gatcagggaa
    57121 gggtctttta aatcctgcac aaacttgccg tcgaccagca cgttaatcaa atcaacaacc
    57181 tgcatttgcg tagcgttgag ttcgtcgagt ttatagcctg tccacaccca gatgtcttta
    57241 cccggacact cggcgcggat gcgttgtacc agtttcagaa tatccggcac gttttgcgga
    57301 tgcagcggat cgccgccgga gagggaaata ccctggcgtt tgatacgagt gtcattcaga
    57361 tcgttaatga tctggtcttc cattgctttg gtaaatggct gaccagaatt tacccgccat
    57421 gtgcttttgt tatagcaacc ggggcattca tgaacacacc cggagacaaa cagggtgcag
    57481 cgagtgccgg ggccgttgac gatgtcgaca ggatagtact gatgataatt cattatttcg
    57541 cctggcttat cagtccgcgt gtaggctgca tccggtaatc aatgcctgat gcgacgctgt
    57601 cgcgtcttat caggcctgga gccgttgcca aatgtaggcc ggataaggcg tttacgccgc
    57661 atccggcatt tgcttagcgc agagtgaaga ttaacctatc tgcccattcc ccaaatgttt
    57721 aacgcggcgc ttaacttctt cctgcttacc agcgttaaac ggacgtgcat ccgggctacc
    57781 taaatatccg cacacgcggc gagttaccga cacacgggag gcgtcatggt taccacattt
    57841 cgggcaagtg aagcctttgc tggtgcactc gaactcaccg gtaaagccac actcgtagca
    57901 ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc tgatagctgt aatcccagac
    57961 atcttccagc gccttcaggt tgtgctgaat gtttggatac tcgccgtagc aaatgaaacc
    58021 accgttcgcc agcggcgggt aaggcgcttc aaagtcgatc ttgtcgtacg ggttcacctt
    58081 cttctccaca tcgaggtgga aactgttggt gtagtaacct ttatcggtca cgcccggcac
    58141 cacgccaaac tcagcagtat cgagacggca gaagcggtcg cacaggtttt cactcggcgt
    58201 gctgtagaga ctgaaaccat aacccgtttc ttctttccac tgatccactg cctgacgcag
    58261 acgttcaaca atggcgatac ctttcgcgcg aagctgctcg ttgtcgtaaa catgctcgcc
    58321 gccgaacagc gcgttaatgg tttcgtggat gccgatgtaa cccagcgaaa tagacgcacg
    58381 accgtttttg aagatttcag aaacatcatc gtcagcattc agacgcacgc cacaagcacc
    58441 ttccatatag aggatcgggg ccacgcgcgc tttcacgcct tcgagacgag cgatacgggt
    58501 catcagcgcc ttacgtgcca gcaccagacg ttcatccagc agcttccaga aggtggcttc
    58561 atcgcctttt gcttccagag caatacgcgg caggttcagg ctgatcacgc cgaggttgtt
    58621 acgaccatcg tggatctgct cgccgttttc attttcccac acgccgagga agctgcggca
    58681 gcccatcggg gttttaaacg aaccggtgac tttcactacc tgatcgtagt tcaggatatc
    58741 cggatacatg cgcttgcttg cgcactccag cgccagctgt ttgatgtcgt agttcggatc
    58801 gcctttttta tggttcaggc catcgcgaat cgcaaacacc agtttcggga acaccgcagt
    58861 tttacggttt ttaccgaggc ctgcgatacg gttacgcagg atcgattcct gaatcaggcg
    58921 cgattcccag ctggtgccca ggccaaaacc aaaggttaca aacggcgtct gaccgttggc
    58981 ggtgtgcagg gtgtttactt cgtactccag cgactggaag gcatcgtagc actctttaat
    59041 ggtacgagag ttagcgtagc cttcggcgtc cgggatgttc cactcttctg cggtcttacg
    59101 atgtttgttg tagctggcgg taacaaacgg cgccagcact tcatcgatac ggttaatggt
    59161 ggtgccgcca taaatatggc tggcgacctg agcaataatc tgcgcagtta ccgcggttgc
    59221 cgtagagatc gacttcggcg gttcaatctc ggcgttcccc atcttaaagc cctgggtcag
    59281 catgcctttc aggtcgatca acatgcagtt gaacatcggg aagaacggcg agtaatcgag
    59341 atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc acgtcacgcg gcagcaggtg
    59401 ctgacgtgca tagtgtttag ccacgatccc ggccagcagg tcgcgctggg ttggaatcac
    59461 cttgctgtct ttgttggcgt tttcgttgag taacgaggcg ttggtctgct cgaccagacc
    59521 acggatctcc tggttcaggc gaccgcgttt ttcacgttca atgtcgcgat cgtgacggta
    59581 ctcgatgtaa gcacgagcca gttgtttgta tggacccgac atcagctggt tttcaactgc
    59641 ggtctggatc tcgttgatat ccacctggtt gcggccctgc atctgctcgc tgacaaccgc
    59701 ggcaacagtg gcgcaataat cggcatcatc gacttccgct gctttagctg cacgcagaat
    59761 cgcttctttg atgcgctctg atttaaacgg cactttgcag ccgtctcgtt tcatcacatg
    59821 cggtgtcatg atcgctccat atttttaaga acaggttatc cacagaaatt gggaaaggcg
    59881 ttttccggtt ttttcgtttc gttttccggt gctttccgca atcgccatcc gcttatccac
    59941 aacgccgcac ctttttcgtg gggagctgta gtagcaatta tagtcgatta atacaacata
    60001 ttgggttggg acgcatttta aagtctatat atagtgcttt gcatcaagga tgttttaact
    60061 ttttttgatg tagctcaaag taaaaagcag agcgtacgga tgacgggcgc tacagcgtta
    60121 tgtaaatttt ttaatgaatt tgctggttga aaaatcaaca aaaacaacat gctgacagac
    60181 aaaaccccgg aatgaccggc attaccgggg cttagggaag atttacttct gtaaccacca
    60241 gacagcctca aaaggccgta aattcatggc acagggttgt ggtgaggctt cttcgtagtt
    60301 atgcatcaca agctgccagt tgccgcgcat ttgccctggc tgccagggtt ggatctcacg
    60361 gctaaggttg gcaatgacca gcaaggtttg ccccttccat tcacggcgat agcaccacaa
    60421 tacagggctg tttggcagca gatcctggta attgccccat gtcaggatgg cttcttgctt
    60481 acgcagtgcg attaactttt ggtaggtgta aaacaccgag gaatcatcgg ccagcgcggc
    60541 ttctacgttg attttttgat agttatcgcc caggccaatc cacggttcgc cagccgtaaa
    60601 cccggcatta tcgccgttgc tccattgcat gggcgtgcgg ctgttgtcac gggatttact
    60661 ggcgaggatt gccaataact cgtcggcatc acgaccatcg ttgcgcagct cggcaaacat
    60721 atttaggctc tccacgtcgc gatagtcagt aatgcgcgtg aaatgcgggt tggtcatgcc
    60781 aatctcttcg ccctggtaga tatacggcgt tccctgcatg ccatgcagca ccatcgccag
    60841 catttttgcc gcaggcacgc ggtattcacc ttcatcacca aagcgagaaa caatgcgcgg
    60901 ctgatcgtgg ttacaccaga acaaggcatt ccatgccacg ttgtgcattc cttgttgcca
    60961 gtggcggaac aatgttttca acgccacaaa gtcaggttta gccagcgtcc atttttcacc
    61021 accgggataa tcgaccttca ggtgatgaaa attaaaggtc atcgacaatt cactgcctgt
    61081 cagtgccgcg tatcgctggc aatgctcaag gctggtggag gacatttcac ctacggtcat
    61141 taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc aaaaactcgt gtgctcgtgg
    61201 cccgtcggtg tagaagcgac gcccgtcgcc atccaggtct tcagggaaac gcgggtcttt
    61261 ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc ccacgatcgg cccagaattc
    61321 acagactttt ttcagctctg cgcgtaccgc tggattctcc cagttgagat ccgcctgttc
    61381 tggtgcaaag agatgcaaat agtactgttc gctttccgca tgccagcgcc acgcactacc
    61441 gccaaatttt gaacgccagt tgttcggtgg cgtttctggt tcgccatcgc gccagatata
    61501 aaactggcgg taagggcttt ctttgttcag cgcctcgcga aaccaggcat gttgggtaga
    61561 ggtatggtta aacaccatat cgagaatgat acgaatcccg cgcgattttg cctgcgtcac
    61621 cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca atcgccgtat agttcgctac
    61681 gtcgtaaccg ttatcgacct ggggagagat ataaaagggg gttagccaga tggcatcaac
    61741 gcccagttta tgcagatagt ccaggcgttg gataacgcca cgtaaatcgc cggtgccgct
    61801 acccgtggtg tcctgaaaac tctttggata aatctggtag ataacgccgt tttgccacca
    61861 gtggggaaga ttagtcatta cgttattcct gcaaatgcga gggggcgcaa ttgcgccccg
    61921 aagaaaatta aacaatatcc agcgtgccca ggcggtattt ccgctgatag ataaacgagg
    61981 tgagtacaat cgggatgatg atggcgataa ccattgccag cgcaaatacc tgccagtagc
    62041 tcggttgaat cgagagaatt cccggcaggc cgcctacgcc gataccattc gccataacgc
    62101 cgttcaggcc gcatagtaat cctgccagac cagaaccaat catcgcgcac agcatcggga
    62161 agcgatattt caggttgatg ccgtacattg caggctcagt gaccccaagc caggcggaga
    62221 tagcggcagg cacggagatc tcgcgttcat tgtgcttgcg gctggaaatg ataatgccta
    62281 tcacggcgga gccctgagcg atattcgaca gcgcaatcag cggccacact ggcgtgccac
    62341 ccatgctttg aatcatctgc aaatcaatag caagggtggt ctggtgtacg ccggtgatca
    62401 ccagcggcgc gtacaggaag ccaaacaatg ccgcaccaat cggagcaaag ctgccggtca
    62461 tcaggtgacg taccgcaaag gcaacgccat cgccaatcat gcgaccaaac ggaccaatca
    62521 gcgcatgggc gaggaacacc gcgaggatca gcgaacagac gggcaccacc accagataga
    62581 ggtaatccgg tacgatgcgt ttaaggcgag tttcaataac gcccagcgcc agcccggcta
    62641 acagtgccgg gatcacctgc gcctgatagc ccactttggc gatgctgaac atgccaaagt
    62701 cccacacttc cggcagctgc tgcccgagca gataagcgtt catcagctgg ggggaaacca
    62761 gtgtcacgcc aagcacgata ccaaggatcg gcgtgccgcc catttttttc accgctgacc
    62821 agcaaatccc gaccggcagg tagaagaaga tcgcttcacc gatcaaccac agaaaatcgt
    62881 agatcgtttg cagggaaggg tacatttgcg ccagcgtttg accgttgctc atgggcaaat
    62941 cgccgatcac attacgaaaa ccgaggatca aaccgccgct aatcaacgcg ggcagcaacg
    63001 ggaagaagat ctccgcgaaa tgagagatca actgctcatg ccatttcata ttctgccggg
    63061 cggctttttt tacctgctct ttatcaacct gcgcctgtcc ggttgacgct attagtgctt
    63121 gatagtaatc acccacgttg gtgccaatca ccacctgaaa ttgcccggca ttggtgaaac
    63181 agcctttcac catggggagt tgctcaattt ctttcggtct ggcattggcc ggttggttga
    63241 ggacaaagcg caggcgagta atacagtggc tcaccgtcgc aatattgccg cgcccgccga
    63301 ccagttcaat caaccgatcg atatccgttt ggtttatttt gctcatcata aagccccatg
    63361 gcagatgaca tttttggttg gctgcagaat atattgcgcg gaaaaattta aaaacgggaa
    63421 cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag gaaacgatca ggacagggtg
    63481 gcggggatga tgatttgttg cggttcgctg cgcccggtta cctgcgcgat caactggcag
    63541 gctgcctggc gtccagcttc ggcgtaacca ggatctacgg tgacgatctc cggatggagg
    63601 aatttcatta acggcgtatt accgacgctc gccagttgca aggtgtcgat gcgttgctct
    63661 tgcaggtatt tacttgcgcc aagtgccagc gtgtcggttg cgcacagtaa cgcggtagtt
    63721 tcaggcgtaa tcacttttgc tacgttctca tagccttgct tcatagcaag gcctggcagg
    63781 gcggcaacgg gatgcagttt atgcgctttg cagaacgcca ggtaggcttc gtgacgtcgc
    63841 ttcccggtcg tcacgtcact atgcggcacg ccgagataac tgatattgcg atgcccctgg
    63901 tcatacagcc gttgcatcag gatcttgatt gccccttcgt cgtcataaca gaccgaagca
    63961 aagccttttg cgtcacgcgc cagcagaacc agcgatgact gccagtgggc taacatttct
    64021 tctgttatgc cggtaaaacc gaacagcact acgccgtcga tattacgccg tttcagcacc
    64081 cccaaatgtt cggcaactaa ttgcggggaa aactgacttt ccatcatgat tggatcgtaa
    64141 ccttgttcat agaacgctgg cagcatggtt tgaacggcga gattttctga caacgaatcc
    64201 agacgggtga caatgatggc gaccacttta tcgctttgcc cacgcatagc gcgtgcagaa
    64261 cgggaagggg aaaatccatg ctgattcatc actgcttcaa cacgctcgcg cgtgcgctgg
    64321 ctcacgccgc tttcgttatt cagcacccgg gaaaccgtag atttccccac gccgcttaag
    64381 cgcgcgatgt ctttgatggt cagccgattt tgcatcctgt tgtcccgtaa cgtgttgttt
    64441 aattatttga ggctaacttt acccgtgcat tcagcaatgg gtaaagtctg gtttatcgtt
    64501 ggtttagttg ccagcaggta ttatatcgcc ataaatgcta cgaatattat tggattctcc
    64561 ttattatttg cggcgctttt ttcacttacc ggaggttata tggaacctga tcccacgcct
    64621 ctccctcgac ggagattaaa acttttccgg taagcccgtc ttttcacggc gttaccggat
    64681 gcgtaaggcc gtgacgtttt aacgtccctg ctcagcttta ttaccttcag gtaaggcttc
    64741 gccacgcctg aagacatttc tgtactgttt cagacagtgc ggagggactc cttatgttta
    64801 aagaaatttt tacccggctc attcgccatt taccttcccg tctggttcat cgtgatccat
    64861 tgcctggcgc gcagcagaca gtgaatacgg tggtcccgcc gtccttaagt gcgcattgcc
    64921 tgaaaatggc ggtgatgccc gaagaagaat tgtggaaaac gttcgacacc catccggaag
    64981 ggttaaatca ggcggaagtg gaatctgccc gcgaacaaca tggtgaaaat aaattacccg
    65041 cacaacaacc gtcgccgtgg tgggtacatt tatgggtctg ctatcgcaac ccctttaata
    65101 ttttactcac cattctcggc gctatttctt acgccacgga agatttattt gccgcgggcg
    65161 ttatcgcgct aatggtcgct atttcaacgt tgctgaactt tattcaggaa gcacgttcca
    65221 ctaaagcggc agatgccctg aaagcgatgg tcagcaatac cgcgacggtg ctgcgcgtaa
    65281 ttaacgacaa aggcgaaaat ggctggctgg agatcccgat cgaccagctg gtgcccggcg
    65341 atattataaa actggcggcg ggggatatga tcccggcaga tttacgtatc ttgcaagcgc
    65401 gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc tctgcccgtg gaaaaagccg
    65461 ctaccactcg ccagccggag cacagcaatc cgctggagtg cgacacgctg tgttttatgg
    65521 gcaccaccgt ggtgagcggc acggcacaag caatggtgat tgctacaggt gccaatacct
    65581 ggtttggtca actggcgggg cgtgttagtg agcaggaaag cgagccgaat gcctttcagc
    65641 aagggatcag ccgcgtcagt atgctgctga ttcgctttat gctggtgatg gcaccggtgg
    65701 tgctgttaat caatggttac accaaaggcg actggtggga agcggcgctg tttgcgcttt
    65761 cggtcgcggt aggcctaacg ccggaaatgt tgccgatgat tgttacctcg acgctggcgc
    65821 gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa acatctggat gctattcaga
    65881 actttggcgc aatggatatt ctgtgcactg ataaaaccgg caccctgaca caggataaaa
    65941 ttgtgctgga gaatcatacc gatatctccg gtaaaaccag cgaacgcgta ctgcatagcg
    66001 cgtggttgaa cagtcattat cagaccggac ttaaaaacct gctcgataca gcggtgctcg
    66061 aaggtacgga tgaagagtca gcgcgctcgc tggccagtcg ttggcaaaaa atcgatgaga
    66121 ttccgtttga tttcgagcgt cgccggatgt cggtggtagt ggcagaaaat accgagcacc
    66181 atcagctggt ttgcaaaggt gcattgcagg aaatcctcaa tgtgtgttcg caggtgcgtc
    66241 acaatggcga gattgtgccg ctcgatgaca tcatgctgcg taagattaag cgggttactg
    66301 atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc gacgaaatac ctgccagcgc
    66361 gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat cctcgaagga tatattgctt
    66421 tccttgatcc gccaaaagag acaaccgctc cggcactgaa ggcattaaaa gcgagtggaa
    66481 ttaccgtaaa aatccttact ggcgacagtg agttagttgc agcgaaagtg tgccatgaag
    66541 tggggctgga cgcgggagag gtggtcattg gtagtgatat tgaaacgcta tctgacgacg
    66601 aactggcaaa tcttgcacag cgcaccacgc tgtttgcccg cctgacgccg atgcataaag
    66661 aacgcattgt gaccttactg aagcgcgaag ggcatgtggt tggctttatg ggcgatggta
    66721 ttaatgatgc gcccgcctta cgcgctgcgg atatcggcat ttctgtggac ggcgcggtag
    66781 atatcgcccg tgaagcggct gatatcatcc tgctggaaaa aagcctgatg gtgctggaag
    66841 agggggttat tgagggacgt cgcactttcg ccaacatgct gaaatacatc aaaatgacgg
    66901 cgagctctaa cttcggtaat gtgttcagcg tgctggtggc gagtgctttc ttgcccttcc
    66961 tgccgatgtt gccgttacac ttgcttattc agaacttgct gtacgatgtg tcacaggtgg
    67021 cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa gccgcaacgt tggaatccgg
    67081 cggatctggg gcgctttatg atcttcttcg gaccgatcag ctcgatcttc gatattctga
    67141 cgttttgcct gatgtggtgg gtattccatg ccaacacgcc ggaaacgcaa acgctgttcc
    67201 agtcgggatg gtttgtggtg ggcttactgt cgcaaacgct gattgtgcat atgatccgca
    67261 cccgccgtgt gccgtttatt cagagctgtg catcgtggcc gttaatgatc atgaccgtga
    67321 tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct ggccagttat ctgcaactgc
    67381 aggcgctgcc gttaagctat ttcccgtggc tggttgcgat tctggcaggg tatatgacat
    67441 taactcagtt ggtgaaagga ttttatagcc gtcgttacgg ctggcagtaa agaataaact
    67501 gggcacgata gccccgattc agattgcaga caaagtaagc tttgttcatg ccggatgcgg
    67561 cgtaaatgcc ttatccggcc aacaaattca ataaattgca ggaatcacgt aggcctgata
    67621 agcgtagcgc atcaggcgat tttgcgtttg tcatcagact taatccgggc atgatagccc
    67681 ggatttccat caagattagc gacgaacagc gatcgcttcg atctcaattt ttacgtcttt
    67741 cggcagacgg gcaacttcaa cgcaagaacg tgccgggaag gtggcgttgt gttcggtgaa
    67801 gaaggcttcg taagtggcgt ttacggttgc gaagtcgttc agatctttca caaacacggt
    67861 ggttttaacg atatcgccca ctttcaggcc agcggcttcg acaatcgctt ttacgttatc
    67921 cagcgactga cgtgcctgtg cagcgacgtc tgccggtact tcgcccgttt tcggatttac
    67981 cgggatctgg ccggaggtga tgatcatatt gcccagatca acgccctgta cgtaaggacc
    68041 gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc atgatttctc ctttattaca
    68101 gcggtaaaaa gtctggctca ttatagggag ccagactttt attaccaacc gcaattaatt
    68161 ggccagcacc acattatggg aaaactcttt ttcacagtat ttgcatttga gcgcgatgtc
    68221 attggcgcgt tttcgcacgg caaagctgga agaaaccggt tcggcatggc tgatacagtt
    68281 gctgttcggg cagaccagca cattgtcgat gcgctctggc agactggggc gcgatttacc
    68341 caccacttca tagttgtcga tacggttaac cgtggcttgc ggcgcataca atgccagttg
    68401 atctacctga tcttcactca aaaaggtatt ttcgattttg atcagatctt tgcggcccat
    68461 ctcgccagaa ggcaagttca gaccgatggt gatgcgctga tccgtttcgg tcagcttgaa
    68521 cagactcaac agcttaaaac cgatctgggc ggggatatgg tcaattaccg tgccgcgttt
    68581 aatagcttca acctgcaatt tattatcgtg tgtcatctct atttcccctt acagtaccag
    68641 atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg aaaatcccgt tgcctgcctg
    68701 ctggaagtac caggcgtgtg gcgttttatc aacatccgtc gcaatctcat caacacgcgg
    68761 cagcggatgc agcactttca tattggcttt ggcgttgtgg agatcgctgg cgcgaagaac
    68821 aaactgcgct ttcacgttgg cgtactcgga cgggtccaga cgctcttttt gcacgcgggt
    68881 catgtacagg atgtctactt ccgccatcac ttcttcaata gagctgtgca gactccatgc
    68941 gatccctttt tcatcgagca tatccagaat gtattgcggc attgccagcg cgtccggcgc
    69001 gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc tgagtcaggg agtgaacggt
    69061 gcggccatat ttcaggtcac caaccattgc gacgtggaga ttgtccagac gcccctgggt
    69121 ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga tgttggttgg agccatcacc
    69181 ggcattcagt accggtacat tgccggaaaa ctcggtggcc aggcgcgccg caccttcctg
    69241 cggatgacgc atcactatcg catcgacgta agtgctgata accgaaatgg tatcggccag
    69301 cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg gagaagccca ccacgctggc
    69361 acccaggcgg tgcatagatg tttcgaaaga gaggcgggta cgggtagagg cttcgaagaa
    69421 acagctggca atgactttgt gcttcaacag ctctggttgc gggtttgctt tcagtttcgc
    69481 cgctgtcgcc agcaccagat taaggtcatc gcgactaagg tcgtttatgg aaatgatatg
    69541 tttctgatat agcggattag ccatctttta tctcctgacg cctgggcaaa aaaaagcccc
    69601 tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa aacggcaggc cagcgtcttt
    69661 tttcagacgc ggtaagacaa aatgtcgaac acactgaacc atacatcctc ccggcaaatt
    69721 gtccggcatt atactcatcg tcagaagcgg tgcaagcatt tgatgcaata ttttgtcagc
    69781 gcaaacggtt tatttgaatt aaaagtcaag gtatatgcat ttttatttga tgtgattctg
    69841 caggggaact gtccttcggt atcaataatt gcagacattc cctgcttttt cctttttcac
    69901 tttcacgcaa tcagatatgc attttattcc attcatccga cttatagggc ggagtgtgaa
    69961 agcgaacggc taacactatt gcttactgct cagggatgcg cgctatcact ttaatttcaa
    70021 aatcaaagcc tgccagccat gtaacaccca ccgccgtcca gtttggataa ggtggggcgc
    70081 taaatatttc atttttcacc gtcatgatgt cttcaaattg gttttctgga tcggtatgga
    70141 agctcgtaac atcaatgata tcgtcaaaag tgcatcccgc agctgccagg gtcgcatgca
    70201 aattatcaaa tgccagtctg acttgttgct gaaaatcggg ttctggtgtt ccgtcctctc
    70261 gacttcctac ttgcccggaa acaaacagca aatcgccgga acgaatagcc gcagaataac
    70321 gatgctcagc atacagtgaa tgtcggccag cagggaaaac agcggttctt tctaccattt
    70381 ggttatcctc aagatttacg acatgaacag aagatttctc tttaccggga gccgctttta
    70441 gcggacgacg tgagtaaaca aaacccagac atcatggata atggctgggc ttaattgagc
    70501 gtagtcggtt atgcgccaaa cgcgccatca atggtatgca tcgtgccggt aacaaaactg
    70561 gcttctggcc ctgctaacca tgcgaccata ccagcgacct cttccggttg cccatgtctt
    70621 ttgatagcca taaaactatg caacatatcg cgcattggcc cgttggcggg attagcgtcg
    70681 gtatcaattg gccctggctg gacgacgtta atggtgatcc aacgcggtcc gaaatcacgg
    70741 gccagcccgc gcgccatgcc ttgcagggca gatttactgg cggcataagc agccatgcct
    70801 gcaacaggca tacgatcgcc attcacggag ccgatgatta agatgcgccc gccttcgggc
    70861 atctgccggg cggcttcaac agaggcatga taaggagcat gaatattgat tttgaaaagg
    70921 cgatcaatat cgtcggcatt caattccagg gcatcgccaa agacgccaat acctgcattt
    70981 accaccagga tatccaatgc gccgctctta cgaacgacat caatgacagc gtctctgtca
    71041 gcactatctg tgaatactgc tgtcgctcca gtctcttgtg ccaggtgttc agcggcatct
    71101 ttcgaccccg cataggtgaa tcgtacattg gccccatcgg tgacgaaacg acgtacgata
    71161 gcggcaccga taccacgact gccaccgagg atgagaactg tcttacctgt aaaagcgccc
    71221 ataaggactc cttgatttat tatgtaacat gcattacaaa actgttttaa ctttctgtca
    71281 acaagttttg taatggtcac taaaaaacaa tctcgcgttc caggtcgtcc cagacgtttc
    71341 gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc accaaaaagg tttcgatgct
    71401 gtcagtgttg ctgaagttac tgattatctt ggtattaacc ccccgagcct ctacgcggct
    71461 tttggcagta aagctgggtt atttagccgt gtactcaatg aatacgtcgg tacggaagct
    71521 attccgcttg ccgatattct tcgtgatgat cgtccagtag gcgagtgcct ggttgaggta
    71581 ttaaaagaag cggcgcgcag atatagccaa aacggcggct gcgctggctg tatggttctt
    71641 gaaggtattc atagtcatga tccactagcg cgtgatattg ccgttcaata ttatcacgcc
    71701 gcagaaacga ccatttatga ctatatcgcc aggcggcatc cacaaagcgc acaatgtgtg
    71761 actgatttta tgagtaccgt gatgtcaggg ctttctgcga aggcacggga ggggcactca
    71821 atcgaacaac tctgtgcaac agctgcactg gcgggggaag cgataaaaac tcttctcaag
    71881 gagtgatgct ggccttgatc cgaaaggcgg gaacgcgcct gccgataagt tgtgataaga
    71941 caataattca cgcgttaagg ctggcggaat tgatcatctt ttcgtataac gatagaaatg
    72001 aaacattgtt ttaattaagg agtggaaaag atgatcatcg gaaatattca taatcttcag
    72061 ccgtggctac cccaggagtt acgccaggcg attgagcata tcaaagcgca cgttacggca
    72121 gaaacgccaa agggtaagca cgatatcgaa ggcaatcgtc tgttttatct tatctcggaa
    72181 gatatgaccg agccgtacga agctcgccgt gcggagtatc atgcccgcta tctcgacatt
    72241 cagattgtgt taaaaggtca ggaaggcatg accttcagca cgcaacctgc aggcacgccg
    72301 gataccgact ggttagctga taaagacatc gcatttttgc cggaaggcgt tgatgagaaa
    72361 acagttatcc ttaatgaagg tgattttgtt gtgttttatc cgggggaagt gcataaaccg
    72421 ctgtgcgcag tgggcgcgcc agcccaggtt cgcaaagcag tagtgaagat gctgatggcg
    72481 tgatgacttt tcgccgtaaa taactccagg tttacggcga gtttgtgaaa agagcgtttt
    72541 ttgatatttt tttgtgagta gaatttgtaa tgcttagacg ttcttattaa ctcaaggagt
    72601 ccgtcatgag caaaatatca ggttggaatt tttctcaaaa cattacatca gccgacaatt
    72661 gtaaacaaaa aaatgaagac ttagatacct ggtatgtggg aatgaatgat tttgcccgaa
    72721 ttgccggagg gcagaatagc agaagcaata ttctttttcc cagagcattt ttggagtttt
    72781 tggctaagat atttaccctg ggttatgtgg attttagcaa acgctccaac gaagcgggta
    72841 gaaatatgat ggctcatatt aagtcctcat cttatatcaa aaataatgat ggtagtgaga
    72901 taatgaagtt tgttatgaat aatcctgaag gggagcgagc ggatttatct aaggtggaaa
    72961 tagaaattac acttgcctca gcttttaata atgggattcg tgaaggacat acagtcatta
    73021 tatttccaca acctgatggt tcgactaatc gttatgaagg gaagtccttt gaaagaaaag
    73081 atgagagttc attacacctg attactaaca agattctggc gtgttaccaa cgcgaagcta
    73141 acaaggaaat agctcgtcta ttaaatattc cgcaggagtt aaataattct caggacttga
    73201 ataactcgca ggtgagttgt aaagattcag ttgattctac gattacggat ttatt
//
LOCUS       DAMZII010000004        70117 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZII010000004 DAMZII010000000
VERSION     DAMZII010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.004"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            60..251
                     /locus_tag="POA77_000221"
     CDS             60..251
                     /locus_tag="POA77_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDI6363722.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            562..1080
                     /locus_tag="POA77_000222"
     CDS             562..1080
                     /locus_tag="POA77_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI6363723.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            1096..1635
                     /locus_tag="POA77_000223"
     CDS             1096..1635
                     /locus_tag="POA77_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDI6363724.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(1728..3305)
                     /gene="guaA"
                     /locus_tag="POA77_000224"
     CDS             complement(1728..3305)
                     /gene="guaA"
                     /locus_tag="POA77_000224"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI6363725.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3374..4840)
                     /gene="guaB"
                     /locus_tag="POA77_000225"
     CDS             complement(3374..4840)
                     /gene="guaB"
                     /locus_tag="POA77_000225"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI6363726.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            5002..6372
                     /gene="xseA"
                     /locus_tag="POA77_000226"
     CDS             5002..6372
                     /gene="xseA"
                     /locus_tag="POA77_000226"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI6363727.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6369..6584)
                     /locus_tag="POA77_000227"
     CDS             complement(6369..6584)
                     /locus_tag="POA77_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDI6363728.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6653..8125)
                     /gene="der"
                     /locus_tag="POA77_000228"
     CDS             complement(6653..8125)
                     /gene="der"
                     /locus_tag="POA77_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI6363729.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8243..9421)
                     /gene="bamB"
                     /locus_tag="POA77_000229"
     CDS             complement(8243..9421)
                     /gene="bamB"
                     /locus_tag="POA77_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDI6363730.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTAPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9432..10052)
                     /gene="yfgM"
                     /locus_tag="POA77_000230"
     CDS             complement(9432..10052)
                     /gene="yfgM"
                     /locus_tag="POA77_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDI6363731.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(10070..11344)
                     /gene="hisS"
                     /locus_tag="POA77_000231"
     CDS             complement(10070..11344)
                     /gene="hisS"
                     /locus_tag="POA77_000231"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI6363732.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQYKDKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11455..12573)
                     /gene="ispG"
                     /locus_tag="POA77_000232"
                     /gene_synonym="gcpE"
     CDS             complement(11455..12573)
                     /gene="ispG"
                     /locus_tag="POA77_000232"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDI6363733.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12600..13613)
                     /gene="rodZ"
                     /locus_tag="POA77_000233"
     CDS             complement(12600..13613)
                     /gene="rodZ"
                     /locus_tag="POA77_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDI6363734.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13898..15052)
                     /gene="trmG/rlmN"
                     /locus_tag="POA77_000234"
     CDS             complement(13898..15052)
                     /gene="trmG/rlmN"
                     /locus_tag="POA77_000234"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDI6363735.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15202..15633)
                     /gene="ndk"
                     /locus_tag="POA77_000235"
     CDS             complement(15202..15633)
                     /gene="ndk"
                     /locus_tag="POA77_000235"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI6363736.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15782..18094)
                     /gene="pbpC"
                     /locus_tag="POA77_000236"
     CDS             complement(15782..18094)
                     /gene="pbpC"
                     /locus_tag="POA77_000236"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDI6363737.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(18095..23056)
                     /gene="yfhM"
                     /locus_tag="POA77_000237"
     CDS             complement(18095..23056)
                     /gene="yfhM"
                     /locus_tag="POA77_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDI6363738.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            23263..24108
                     /gene="sseA"
                     /locus_tag="POA77_000238"
     CDS             23263..24108
                     /gene="sseA"
                     /locus_tag="POA77_000238"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDI6363739.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24601..25377)
                     /gene="sseB"
                     /locus_tag="POA77_000239"
     CDS             complement(24601..25377)
                     /gene="sseB"
                     /locus_tag="POA77_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDI6363740.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25519..26802)
                     /gene="pepB"
                     /locus_tag="POA77_000240"
     CDS             complement(25519..26802)
                     /gene="pepB"
                     /locus_tag="POA77_000240"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDI6363741.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGGRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26981..27181)
                     /gene="iscX"
                     /locus_tag="POA77_000241"
     CDS             complement(26981..27181)
                     /gene="iscX"
                     /locus_tag="POA77_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDI6363742.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27193..27528)
                     /gene="fdx"
                     /locus_tag="POA77_000242"
     CDS             complement(27193..27528)
                     /gene="fdx"
                     /locus_tag="POA77_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDI6363743.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27530..29380)
                     /gene="hscA"
                     /locus_tag="POA77_000243"
     CDS             complement(27530..29380)
                     /gene="hscA"
                     /locus_tag="POA77_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDI6363744.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29397..29912)
                     /gene="hscB"
                     /locus_tag="POA77_000244"
     CDS             complement(29397..29912)
                     /gene="hscB"
                     /locus_tag="POA77_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDI6363745.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(30008..30331)
                     /gene="iscA"
                     /locus_tag="POA77_000245"
     CDS             complement(30008..30331)
                     /gene="iscA"
                     /locus_tag="POA77_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDI6363746.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30348..30734)
                     /gene="iscU"
                     /locus_tag="POA77_000246"
     CDS             complement(30348..30734)
                     /gene="iscU"
                     /locus_tag="POA77_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDI6363747.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30762..31976)
                     /gene="iscS"
                     /locus_tag="POA77_000247"
     CDS             complement(30762..31976)
                     /gene="iscS"
                     /locus_tag="POA77_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6363748.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(32088..32576)
                     /gene="iscR"
                     /locus_tag="POA77_000248"
     CDS             complement(32088..32576)
                     /gene="iscR"
                     /locus_tag="POA77_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDI6363749.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32877..33614)
                     /gene="trmJ"
                     /locus_tag="POA77_000249"
     CDS             complement(32877..33614)
                     /gene="trmJ"
                     /locus_tag="POA77_000249"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDI6363750.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33733..34536
                     /gene="suhB"
                     /locus_tag="POA77_000250"
     CDS             33733..34536
                     /gene="suhB"
                     /locus_tag="POA77_000250"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDI6363751.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34681..35535
                     /locus_tag="POA77_000251"
     CDS             34681..35535
                     /locus_tag="POA77_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6363752.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35726..37006
                     /gene="csiE"
                     /locus_tag="POA77_000252"
     CDS             35726..37006
                     /gene="csiE"
                     /locus_tag="POA77_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDI6363753.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36998..38137)
                     /locus_tag="POA77_000253"
     CDS             complement(36998..38137)
                     /locus_tag="POA77_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDI6363754.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38297..39187)
                     /gene="hcaR"
                     /locus_tag="POA77_000254"
     CDS             complement(38297..39187)
                     /gene="hcaR"
                     /locus_tag="POA77_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDI6363755.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39323..40684
                     /locus_tag="POA77_000255"
     CDS             39323..40684
                     /locus_tag="POA77_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDI6363756.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKATSDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40681..41199
                     /locus_tag="POA77_000256"
     CDS             40681..41199
                     /locus_tag="POA77_000256"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDI6363757.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41199..41519
                     /locus_tag="POA77_000257"
     CDS             41199..41519
                     /locus_tag="POA77_000257"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDI6363758.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41516..42328
                     /gene="hcaB"
                     /locus_tag="POA77_000258"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41516..42328
                     /gene="hcaB"
                     /locus_tag="POA77_000258"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDI6363759.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42338..43540
                     /locus_tag="POA77_000259"
     CDS             42338..43540
                     /locus_tag="POA77_000259"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDI6363760.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43637..44059
                     /locus_tag="POA77_000260"
     CDS             43637..44059
                     /locus_tag="POA77_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6363761.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(44107..44979)
                     /locus_tag="POA77_000261"
     CDS             complement(44107..44979)
                     /locus_tag="POA77_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDI6363762.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44991..46085)
                     /locus_tag="POA77_000262"
     CDS             complement(44991..46085)
                     /locus_tag="POA77_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6363763.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(46118..47116)
                     /locus_tag="POA77_000263"
     CDS             complement(46118..47116)
                     /locus_tag="POA77_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6363764.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(47141..48652)
                     /locus_tag="POA77_000264"
                     /pseudo
     CDS             complement(47141..48652)
                     /locus_tag="POA77_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            complement(48675..49658)
                     /locus_tag="POA77_000265"
     CDS             complement(48675..49658)
                     /locus_tag="POA77_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI6363765.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     STVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49755..53036)
                     /locus_tag="POA77_000266"
     CDS             complement(49755..53036)
                     /locus_tag="POA77_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDI6363766.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQSCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            53154..54347
                     /locus_tag="POA77_000267"
     CDS             53154..54347
                     /locus_tag="POA77_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6363767.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54411..55664)
                     /gene="glyA"
                     /locus_tag="POA77_000268"
     CDS             complement(54411..55664)
                     /gene="glyA"
                     /locus_tag="POA77_000268"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI6363768.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55991..57181
                     /gene="hmpA"
                     /locus_tag="POA77_000269"
     CDS             55991..57181
                     /gene="hmpA"
                     /locus_tag="POA77_000269"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDI6363769.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57226..57564)
                     /gene="glnB"
                     /locus_tag="POA77_000270"
     CDS             complement(57226..57564)
                     /gene="glnB"
                     /locus_tag="POA77_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDI6363770.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57625..58959)
                     /gene="glrR"
                     /locus_tag="POA77_000271"
     CDS             complement(57625..58959)
                     /gene="glrR"
                     /locus_tag="POA77_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDI6363771.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58949..59662)
                     /gene="qseG"
                     /locus_tag="POA77_000272"
     CDS             complement(58949..59662)
                     /gene="qseG"
                     /locus_tag="POA77_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDI6363772.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59827..61254)
                     /gene="qseE"
                     /locus_tag="POA77_000273"
     CDS             complement(59827..61254)
                     /gene="qseE"
                     /locus_tag="POA77_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDI6363773.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61830..65717)
                     /gene="purL"
                     /locus_tag="POA77_000274"
                     /gene_synonym="purI"
     CDS             complement(61830..65717)
                     /gene="purL"
                     /locus_tag="POA77_000274"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDI6363774.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLEHLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRNVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65975..67531
                     /gene="mltF"
                     /locus_tag="POA77_000275"
     CDS             65975..67531
                     /gene="mltF"
                     /locus_tag="POA77_000275"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDI6363775.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67528..68064)
                     /gene="tadA"
                     /locus_tag="POA77_000276"
     CDS             complement(67528..68064)
                     /gene="tadA"
                     /locus_tag="POA77_000276"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDI6363776.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(68089..68724)
                     /gene="yfhb"
                     /locus_tag="POA77_000277"
     CDS             complement(68089..68724)
                     /gene="yfhb"
                     /locus_tag="POA77_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDI6363777.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68933..69781
                     /locus_tag="POA77_000278"
     CDS             68933..69781
                     /locus_tag="POA77_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6363778.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69837..70097
                     /gene="yfhL"
                     /locus_tag="POA77_000279"
     CDS             69837..70097
                     /gene="yfhL"
                     /locus_tag="POA77_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDI6363779.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16632 a        19341 c        17657 g        16487 t
ORIGIN      
        1 ctgttgtgcc ttaatgtaag taccgtccac ggcgtgggac atacttcaag gaaccttttg
       61 tgagtcaggc aaccagtatg cgaaaacgac accgatttaa cagtcgcatg acccgtatcg
      121 tactgctcat cagctttatc ttcttctttg gccgttttat ctactcgtcc gtcggtgcct
      181 ggcagcacca tcagagcaaa aaagaagctc agcaatccac actctccgtc gaatcaccgg
      241 tacaacgtta gcggttacct tctccacttt cacagaacat aacggcactt cgctgtcgga
      301 tgcttttgct gtttgggatt atcaaagcgg cagatattct ttcatcttaa attttacgtc
      361 tttatcctga ctgatgttta tcctgtttgg ctgcgaaata aatataaaat taatatatat
      421 gttgtaatga tatattttta taaattattc cctgcgtgaa ttttaataaa tttaatctat
      481 ccctttatac gcaatacatt tactttcctc ttttgatgat cttaaatgtc ttatttttcg
      541 taatgtgtat aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg
      601 ttagcgtcat tcactctggc aggatgccag tcaaatgctg acgatcatgc tgccgatgtt
      661 tatcaaaccg atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt
      721 cttcccgcaa aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc
      781 gcgcttattg gcgcagtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat
      841 tccggaacga cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg
      901 gtgaatgata aaaccttagt ggaaggtgtt tctttaacct ataaggaagg caccaaagtg
      961 tatacctcta cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt
     1021 accacgacgt ataacgaaac gcgtattcag ccaaatacca aatgtcctga aaagagctaa
     1081 taatcaggag gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat
     1141 ccggctatcg cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa
     1201 ggtaaaaatg aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc
     1261 cagcaagaga agcaacgtcg cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg
     1321 gcagcggcaa ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa
     1381 aaacgcgatc aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg
     1441 gcgctggcga aagaagaagc ccgcgttaag cgcgaaaacg aatttatcga tcaggaactg
     1501 aagcacaaag ctgcgcaaac cgatgtggtg caatctgaag ctgacgccaa cagaaatatg
     1561 actgaaggcg gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa caaatcggaa
     1621 agctggttta attaatcgat gttagtaact tcaagcctat gattcttgaa gataaaaaac
     1681 cctctgtagt aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca
     1741 atggtagctg gcggcttgcc gctgatgtca tacaccacgc gagaaatacc gttcacttca
     1801 ttgataatgc ggttggaaac acgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg
     1861 gtcataaagt cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca
     1921 tcgcccatta cgccaacgga acgtaccggc aggaacacag tgaacgcctg gctgactttg
     1981 tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg
     2041 tcacagtact ctttcttcac ttcacccagc acacgaacgc caaggcctgg tcccgggaac
     2101 gggtgacggt acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg
     2161 tctttgaaca gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg
     2221 cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc cgcagattcg
     2281 ataacgtcag ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct
     2341 tcttcatcga acacttcaac gaaaacgcgc ccgatgattt tacgttttgc ttccggatcg
     2401 ttttcgccag ccagcgctga caggaagcga tcttctgccg gtacgtgaac aatgttcaga
     2461 ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg
     2521 ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt
     2581 acggaggaat ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg
     2641 cggatgcgag ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg
     2701 cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc
     2761 gggtggaact gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag
     2821 ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg
     2881 ctcatccaga catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga
     2941 accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag
     3001 gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat
     3061 accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg
     3121 ctttccgggc cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct
     3181 gtcacatccc acgcccacag ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc
     3241 agttgagtgt actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc
     3301 gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt
     3361 cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt
     3421 cgtgaacgtg gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac
     3481 gcagttcgtc gatagtacca cagccggtca gacccataca ggagcgcagg ccgcccatct
     3541 gctggtgaat gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg
     3601 gcaccagttt gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca
     3661 tcgcgcccag ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga
     3721 tttcacccgg agattcttca gtacccgcca gcatggaacc taccatcacc gcgcttgcgc
     3781 cagcggcgat agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg
     3841 gaataccggt gccttccagg gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac
     3901 ctacgccagt aacgatacga gtcgtacaga tagaaccagg gccgataccg actttaaccg
     3961 cgctgcaacc agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga
     4021 tgatttgcag atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg
     4081 agtgaccgtg ggaggagtca atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa
     4141 cacgctcttc gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct
     4201 cgtctttaca ggcgttcggt ttacgttccg ctttctggaa gtctttcacg gtgatcatgc
     4261 cgatcaggtg gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt
     4321 ttgccagcac cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg
     4381 tcatgtaaac gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga
     4441 taatacccac cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct
     4501 cagtcagttc tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca
     4561 ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca
     4621 tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt
     4681 ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca
     4741 gctgggtgct gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa
     4801 cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag
     4861 tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt
     4921 tgcaaaaaat gcttgctatc cccgaagggc gggttactat cgactgaata acctgctgat
     4981 ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt
     5041 cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc
     5101 ggcgaaatct ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac
     5161 gacaccgccc aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgt
     5221 ccacagcatg gacaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggt
     5281 gactaccaga taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag
     5341 tacgaacagc tcaaagcgaa gttgcaggct gaaggtttgt tcgatcagca atacaaaaaa
     5401 ccacttccct cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta
     5461 catgatattt tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat catctacccc
     5521 accgccgttc agggcgatga cgcaccgggg caaattgttc gcgccattga actggcgaat
     5581 cagcgcaatg agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta
     5641 tggagtttta acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc
     5701 agcgccgtcg ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg
     5761 ccaacgccgt ctgccgccgc tgaagtagtg agccgtaatc agcaagagtt actgcgccag
     5821 gtgcaatcga cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgcacg
     5881 cgtcgcttta cgcagatcca tcaccgatta cagcaacagc atccgcagct ccggctggca
     5941 cgccagcaaa ccatgcttga gcgcctgcaa aagcggatga gctttgcgct ggaaaatcaa
     6001 cttaagcgta ccgggcaaca gcagcagcgg ttaacacagc ggctgaatca gcaaaatcca
     6061 cagccgaaga ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa
     6121 accctgcgcg tacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc
     6181 gaagccgtaa gcccactgtc aacgctggcg cgtggataca gcgttactac tgctactgac
     6241 ggcaatgtac tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg
     6301 gaagacggct ggatagaaag tgaagttaaa aacatccagc cagtaaaaaa atcgcgtaaa
     6361 aaggtgcatt aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc
     6421 tgacagaagt aatctactgc accgcaggct tttaacacct gcaacggttg atggcagtct
     6481 ggacaaagag ctttcatttc aataatttcc ccgcaggatg ggcaacgcgc atggccgtta
     6541 tcctgatcaa gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta
     6601 gccatccctc tacattcata gagggaatgg cagataaaat acttacggat aattatttat
     6661 ttttcttgat gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt
     6721 tacgcttgtt cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca
     6781 ttacgtccag cgatttgcgg aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt
     6841 ctttcacctg attaccgtga atcaccacaa tcggcgggtt ataaccacca gcgtgggcat
     6901 atttcagctt cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg
     6961 tcatgatgcg cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac
     7021 gtactgattc aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac
     7081 gagcaaaatc gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt
     7141 cctggctcag gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa
     7201 taaagcccag cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca
     7261 tcaccacgtt ggcatcttca atggcctgca acgttttgat tacggagaat ttctctacag
     7321 catcggtgat tttgccgcgt ttacgtacgc cagcggtatc aatgagcaca tactcacgtc
     7381 catcgcgttc cattggaatg tagatactgt cacgcgtcgt gccaggcatg tcataaacaa
     7441 caacgcgctc ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac
     7501 ccacaatcgc cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt
     7561 cgccgttctc ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt
     7621 gcggtgcgag atcttccatc cacggcagca gcacatgctc cagcagactt aatacgccac
     7681 gaccgtgaga cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca
     7741 ctgcctgatc gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac
     7801 gggagcgcag atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca
     7861 ccataaacag tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg
     7921 tttctacacc gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc
     7981 cttcaatttc cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca
     8041 gcgcatctcg ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc
     8101 cgacaagcgc gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac
     8161 gcacgctata ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt
     8221 tcaaagtgaa cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct
     8281 ggatcagcag tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt
     8341 tttgctgggc aacgaaacga ccatcttcga cgttaatcca gtgcagataa ccttcactgt
     8401 caccgaccac caggttgcca ttatacagca ccggagaagt cagcaggcga tgcagcagat
     8461 cgctttgtgc ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt
     8521 gatcgaccag atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc
     8581 gtttccacat aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca
     8641 gcgcgaaaac aacgccgtta acaacgacgg gagccgtgtc aacatcgctc agacggtcaa
     8701 tttcggtaga accggtcgcc tgggaaatac gctgctgcca aatcatctgg ccctgttcca
     8761 tcagcactgc gctgacgcga ccattatcgc cccccacgac ggccgcacca aaagccgttg
     8821 ccggcgcaga ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga
     8881 cagcgccgtc agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca
     8941 gaccgtcgct gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca
     9001 cagtaccatc gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat
     9061 gcccaccaga cacggtcaca ccgccagaaa gtaatgcagg ctctttagag aaccagccat
     9121 ctttctcggc caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta
     9181 ccaaaccagc gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg
     9241 aatagaagtt gccaatgccg ctaccaacgg aagtactcca cgccgtggtc ggcgtaaact
     9301 ggttttcaac ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg
     9361 aacagccgct taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca
     9421 tcgggtccct ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc
     9481 ggagtaacat cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct
     9541 ttgctcagca atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg
     9601 atggtatcaa gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca
     9661 agacgcagat ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt
     9721 aactgggcgg cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa
     9781 gaagccagcg caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg
     9841 ctatccggtt tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag
     9901 cgtgcagaat caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc
     9961 aaaatcaccc caacagccag tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct
    10021 acctggtcgt tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta
    10081 acgtgcgcaa atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca
    10141 aatccttcac tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc
    10201 gggcacccca tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg
    10261 tcatcaattt cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag
    10321 attgtgtatc agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt
    10381 ccggattaac ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag
    10441 ccggtgttgc acgaccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca
    10501 cggtgccctg ggagccgaga ctgttagtca cccactcgaa aacggtacgg ttatagtaat
    10561 ccagaccacg caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt
    10621 tgcacagacc ggcaaaatgc tcacgagatt cctcgtccag ataatcacct aatgccggag
    10681 cgtcgttgag aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag
    10741 tgtacatgcg gcgtttgcag tcttcgtcca gcttatcttt atactgctca aggaatgcca
    10801 ccagcgcatc gcggtaattg gcgcgtgctt ccagcgaacc gatagagttc agctcaagag
    10861 ttacgtgctc ggaaataccc agcgcacgcc accagcgggc ggtgagcata atcagttcag
    10921 cgtcgatatc cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat
    10981 aacgcccttt ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt
    11041 cctgattgta cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag
    11101 ggcgcagagt caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca
    11161 cgtcggtgac ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc
    11221 ggatttcact gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc
    11281 agatggccgt ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg
    11341 ccacgtttat tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttcg
    11401 ggagctatgc gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt
    11461 ttcaacctgc tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat
    11521 gcgtgcttcc agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc
    11581 ttcatagagg ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc
    11641 acctgggcca ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc
    11701 caggcgttgc tccagcgcgt taaccgtacc gataacatca aattcctgac gcgaacaggt
    11761 cgggcaggcg atgaagttga tcccgcgcga acggatacgc agcgatttca aaatatcgaa
    11821 accgactttg atctcttcga ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat
    11881 gccttcagac agcagcagac ctaaaccaat ggcggatttt actgccccgc tgcgtgcacc
    11941 accggcttcg gtgatcccca gatgcagcgg ctgatcgatc tgttttgcca gcaaacgata
    12001 agactcaaca gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt
    12061 caggcgatcg agatgatcaa catggcgcat ggcggattcc agcaacgcct gcggcgtcgg
    12121 ttcgccatac ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatacggat
    12181 cggaatgttt ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat
    12241 attgccaggg ttaatacgca gacaatcgac gccgtattcc gctactttca gtgcaatgcg
    12301 atagtcgaag tggatgtcag ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa
    12361 cgcttctgcc gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc
    12421 cagcgccttg atttgattga ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga
    12481 ctgtacggcg atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga
    12541 ttttctacgt tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt
    12601 tactgcgccg gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata
    12661 aaacgactca gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca
    12721 attttcagtt tgtatggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata
    12781 ccgctaaaca attttttacc ggtagcatca gtaacctcca gccagcaatc ggcagtaaag
    12841 ttcatcacca gcgcattcgg atcagccgcc ggcgtagtca cgcctgcctg atcggttggc
    12901 aacggcgcag cgccatctgg cgttgttgtt gccgttggtg ccggggtcgc ggcggtatca
    12961 acatttgcct gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc
    13021 gcagttacgg caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg
    13081 gacgttgtag ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg
    13141 ctattactgc tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga
    13201 gctttgtggt cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc
    13261 caggtgaagg tcatcagcca gccgtcacgt tttttgcggc gtttaccgag ggaaaaactc
    13321 tgcatcggcg caactttcgc agcccgtagt ggagcctgct tttccagccc tggcagcagt
    13381 tcttcttctg gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt
    13441 gaagcaagat cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg
    13501 caaagtcgct cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg
    13561 cgagcgccgg tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc
    13621 aggtacgttt aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa
    13681 acatccgtta agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag
    13741 cccacattca ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa
    13801 aaacgcaccg ttaatattga ccagacaatt gcaacttaat acttcattca taaaagatta
    13861 cgccacggta cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg
    13921 cttcaccctg catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca
    13981 actgaccaca ggcagcatcg atatcatcac cacgggtttt acgcacaata gtggtgaaac
    14041 cgtagctcat cagcactttt gagaaacggt cgatacggct gttcgagctg cgtccatacg
    14101 gcgcgcccgg gaacgggttc cacgggatca ggttgatctt acacggcgta tctttcagca
    14161 gttccgccag ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc atcacatact
    14221 caatagtgac acggccctga ttggcgttgg atttctccag ataacggcgc accgcagcaa
    14281 ggaacgtttc gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt
    14341 tcggcgcgtg cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg
    14401 ccggaactac accggaagta gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat
    14461 catcaagcat gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca
    14521 tgcccatcat cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga
    14581 ttttcgccgc acgccacacc tggccaataa tttccgatac ccgcaggttg cggttaaagc
    14641 cctgctgggc ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc
    14701 agagcgtggc acggtcgtct tccgggatat acaccgtttc gacgcgctga tcgcctacgg
    14761 cgatcgccca tttaatggtg ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc
    14821 ggatttccgc cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat
    14881 caaagttgtc gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt
    14941 tttcacctaa atctttaaaa aactcccgca tctgctgacg gttgagatcc agcaggttga
    15001 tttttccatc tttcgtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt
    15061 ccggcctcgt tattacacgt tatggcccct ggagggttga aaaaagaaac gccccggtga
    15121 gcggctgctc gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg
    15181 cacgcggcat ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccaaagaa
    15241 ataagcgatt tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt
    15301 caggctgtca gcgtaatcag cgcgcagagt accagccagc gcgtttgccg gattggtcgc
    15361 gcccagcaga tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat
    15421 cgggccagag gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc
    15481 ataaaagcca cgtgcctgtt caacggtcag gtgcagcatt ttggtgccga caattttgaa
    15541 ccctgcagct tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg
    15601 tttgatgatg gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt
    15661 tgtctgtacc agcgtacgag atggcgcgga ttataatgag caacagggcc gttgactatt
    15721 gatgaaggta acattttttt aaaataaaat gagttttagc aacaatcagg agcaaaacag
    15781 actattgcat gacaaatttc actgtcgcga tttgccccac atcatccatc accagcaatt
    15841 gataatcacc tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt
    15901 cgccattcag aaaccaccag cgttcacctg cccctccact tgattgcaac ggcaaagtcg
    15961 cttccgctgc gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca
    16021 gagggagctt ggcatcgtga ccgtatggcg ggcaaattgt cgaggccggt ggtaagcgca
    16081 cagcgcgacg ttctgatgcg ggcagccagg gttccagcgg tagcggccag acatttatca
    16141 tttcttgtcg cgcttgcggg caatcagcgg caacacgttt gccattttca tccagccaga
    16201 tggggaaacg aatgccatta atgccttcct gctccggcag taatagagtt ggcggctgac
    16261 tcccgtccag cagccaggtt gccaggcggc ggcgacagtt accgtcacct tccggcaaag
    16321 actgtccgcc cggccagcag ataacgccac gagtgactga gttcgggcgc gggtcttccg
    16381 gcagattcgc actgcgcgac agtaagatat tattgacctg attcaacaat ggtacggcac
    16441 tggcaaagcc aaactgacca acaacgggcg tgccgtccgg tctgccagtc cagatcccaa
    16501 tgacatagcg agcgttaacc ccaatcgccc aggcgtcacg atagccatag ctggtgcccg
    16561 ttttccatgc cagtggggcg acgcgcggca aggcactatc cggcaagggt ttcgcttcat
    16621 cagccataat ccggcgaatg atccacgccg cccccgacga cattaaaggc cgttcaagca
    16681 gcggatcgtc aggctgtaag cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat
    16741 acgctgccgc catatcttcc agttttgcac cagcgccgcc gagaatgagt gaaagattcg
    16801 gcgcagcacc gttgggcaaa tataacggca atccaacatt gcgtaacttt gccgcaaacc
    16861 gtttcggtcc ataggcttcc agcacctgca cagcaggtaa gttcagcgag cgcaccagcg
    16921 cctcgctcat gctgatcggg ccatgaaaac cgctatcaaa gttacctggt cgataatcac
    16981 cggtgcgccg ggggacgtct tgcagcagtg atgccgggtg gatcaagcct tcatccagcg
    17041 ccagaccata aacaaacggt ttgagcaccg atcccggcga tcggatcgca ttgaccatat
    17101 caacatgacc aaagcgtgaa tcatcgttga gatcaaccga tcccacccag ccgcgaacac
    17161 gcatatcggt atgatcaacc acgatcatcg ccagtgagct gcgcggtggc aaccgccctt
    17221 tccagttttg cgccagttct tccagacgtc gttgaagacc ggcatccagc gtagtaacga
    17281 ttttgtcgct tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca
    17341 tttgtcgggg tgccagccag atgggttctt cccttgactc ttttacctgc tcacgggacc
    17401 acacaccttg cacggccatc cgttcgagca ctttattacg cgcggcttcg gcacgctccg
    17461 gccaacgatc cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc attgccgcct
    17521 cggaatagct taaattcgca ggcgactttc cgagataagc ccaacttgcc gcaccgatcc
    17581 cctgcaacgt accgccaaac ggagcgcggt taagatacaa ggtcagaatt tcacgcttag
    17641 acagatgcca ttccagttgc aacgcgcgcc agagctggcg aattttgccg ccaaatgttt
    17701 tggggtgagg atcaagcaga cgagcaacct gcatagtgag cgtgctgcca ccggaaataa
    17761 cccgtcccga agtgagatct tgccatgctg cgcgcgccac cgagaatgga ttcacccccg
    17821 gatgcttcca gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa
    17881 catcttcgat tgttaccgga taacgccaga tgccgtcagc atcggcgaag cgccatagcg
    17941 gcgtaccatc ctgcgccacg accactcgtg cgggattgac ttcatgcagc ggtagaggcc
    18001 agagtttatc tgctccccac gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta
    18061 tccagcagcc gcgtttggtt aacaagcgag gcatttacgg tcggacaatc agcagatctt
    18121 cagccgcgcc ggtcgcccgc cattggggaa catacattga ttccaccatc ggttgcggta
    18181 cctgatacgt tcccggcgtc accgcccgcg ccagatacac cagcgttacc ggttggtatt
    18241 catcaacggc aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc ttaatgctcg
    18301 cctgctgcat ctggttcagt aagttttgca cttcgccacc actttgctcc aggctggcgc
    18361 taccgttcgc cagattctga ttttccagtt ccagacccgc aggcagcaga tccacgacta
    18421 acgcatccgg cacgctgtta ctggctttta cctgcaacca gaccagcacc agatcgccgc
    18481 tacgtaacga gtccagcgat ttgctcttac catcagtacc aagaatatga cgctcgattt
    18541 gcagcacatt gttcgccggt aaaggtgcgg attgcggata accgctggca tccatacgca
    18601 accataacgg ctgatcgcca ctgtttctca cctgcaaggt ggcaagttga tcgctattca
    18661 gattgctgtt tagcgttttc tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct
    18721 gccatttacc cggtaaatcc tgaatcgtac gggcagccag gaacaacgcg ttactttcct
    18781 gcgtcgatag ccagcgttca ccaaacgcct gctgcgaaag tgtgttcagc aaagtgtact
    18841 gctcatcggg tagtagttta ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca
    18901 gtggactacc gtaatcaccc agccatatcc gctcatcact atttctcggc gttttcagcg
    18961 ccagcacaat cgcttcttca ccacgcgttg catcacccat ggttttcagc gcaacgccaa
    19021 gttgcagcag cggtaaacca gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg
    19081 cacccagtgg tgctttttgc tgacgggcca gcaccagcgc agcgtaagac tgaacggcga
    19141 atttactggc tttgagatta tccgcgtacg ggatcgacat catgcccgga tcttgtaaat
    19201 agcgcagcag acgctcatta ccccggttaa tggcgtctgt cggcacgctg taaccctgtt
    19261 cgcctgcgcg gaccaggaaa tccatcacgt aagccgtcag ccagtactct tcgtcaccgt
    19321 ttttatccca cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc
    19381 cgatatcgac cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc aacgcctgca
    19441 gttgggctgc gttggtataa agtgacggga acaggccgct ggcggtttgc tcaagacagc
    19501 cgtacggata cgcttttaac tctttgatat aacgtgcaat gttcagcggt ggtttgccgc
    19561 tcaacaacaa ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa
    19621 tcgcccatgt ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg
    19681 ccggacggac gccgattttc cactgcttat gctgatcggc aacggtttca cccggtaacg
    19741 ctaacccgct aatggtggcc tgaatttctc catcgccata acccggcaat gctcgcaccg
    19801 ggataaacag cgtagtacgc acacctggcg ctaattcaac ggctgcgggt gaatcgctga
    19861 ccagttcaag caaaccactg gcggtcaggg caacgttcag tttttgcggt ttatcggtaa
    19921 gattagtgat atccagcgtc agacgcgagg tatcgccact cgccataaag cgcggcatgt
    19981 tcagttcagc aatcaccggt gcggcaacta tcactttgct ttcgttgcta ccgaaatcat
    20041 ctgccgtcca ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca atcggcagtg
    20101 taaccgagcc ttcgccctgt tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat
    20161 tgacgtgatt gaccggcggt ttaccaccac gtttcagctc atcaccatcg ccaccgaaac
    20221 gcagagctgc cagacgcccc tgaccttcaa taacctgacc gtaaatatcg taaatgtctg
    20281 cgccatagcg tttctgacca aagaacgcct gccacggatc tggcgtgacg tagtcagtaa
    20341 tattcagcac accactatca acggcagaca ccagcacatt cacctgttta ggcttctcgc
    20401 catttttagt gctggcttta attttcacgg ttaatggttg attgggacgc atttttgctg
    20461 gtgtttccag cgccagatcg aggcgacggt tttcatcgcc aagcggcaga tgcaacacac
    20521 caaccgcgcg ttttggcgtc gcggagcgag atttatcgcc aggacgtacc accagcgtac
    20581 ttaaatacag atcatgacga ttccaggttt tatcgaccgg aatcgtcaga tccagccctt
    20641 gagcccgaac atcaatctct tgccaccaca gcggcccttc actggactcg accatcgcat
    20701 aacctttacc cgccgttggc gcggcgatat gcaacttaat ggtgtcgcca gggcgataac
    20761 tggctttatc cagtttcagc gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt
    20821 tgtcctgcca gctatagcca gcccagaaac gaacactact gaccgcttca ttcggcgctt
    20881 tgacttccag acgataagcg ccccactcta ccggaaaact gaccttgccg gtttcgtccg
    20941 ctttcagatc cagagtttgt tcattttcga tcagatcttt ttgatcaaac tgtgactgcc
    21001 agccttcatc ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct
    21061 gcaagcccga cacggctttt ttcacgcctt gcgcatcgct ataaacgatg tcaaaaccgg
    21121 cgttactgcc ttcatcaaca atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa
    21181 ccgatttcga ggcgaactgc ggacggatcc ccggcaatgc atcggcaggc cagatagcct
    21241 gctcagcgcg gcgcgtcacc gggcgaccgc ccgattccag caaactaccc tggaaaataa
    21301 cctgtaatgg ggaatgcgtt tccttccact ggctttctgt agaaacttcg ccgcgccctt
    21361 tatcatccag cgtcaactga acttcatcca gcgtgcggga aagattttcg gcagctatat
    21421 cgccgaattc aaaaccaggt aaggctgaca cagcttcacg cagtgggcgc aggaaaagtt
    21481 gcccttgcaa agtattacca ttagcaggtg caccatacag gtagtacccc accacggaga
    21541 atttcacttc atctttcggc gttagcgggg ttttctcacc ggtcagattc agcgccatgc
    21601 gctctggcat aaaatcttcg acgtggaaat cccacatccg atactggtta tcgcccgtgt
    21661 tagcgcgaat atgccacata ccggttgccg cattgctatc gagtggccag gtaaagtggt
    21721 agaggccatt ctccggctga ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca
    21781 cgtctaactt gatgggttga ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat
    21841 tgaggattac cgtttcacct gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt
    21901 tgctatagcc tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc ggaagtttta
    21961 aatcgagtag cgttgtctga ccgtctttac gcgccaacag taatgccgcg tttttatcat
    22021 tttccagctg cacatgcccc tgagcgtcac tggttgcctg agtcagagtc tgccctttct
    22081 catttaataa agagacttca attccttgct gggccgcgcc gttttccaga ctttgggtaa
    22141 agatatccag acgattgtga taacggtgag ctgaaacgcc gatatcactt aacgtaaaca
    22201 gcgtcgcggg attactgtaa tcgtaacgtc cagcctgatt catcacagcc agatacacgc
    22261 ccgcctgctg aagcggtttg atatcgccca gcggcagcaa taatttttca cgggtgttac
    22321 gcgcaggatt gagatcaaac cgtccggtgt agaccagatc cgccatctgc agcagtttgt
    22381 ctgactgcca gttcgccagc gaattgcggt attcccattg gctaatgaat gctggcagag
    22441 attctggctt aacgcggaag aagttaacat cgacattatt aacgttgagc gccattaccg
    22501 gcagcccttc aacgactttg ccaggcagca gcgaaccacg gctggcaaaa ccgacgctgg
    22561 gttggatgtc gcgggtagtt atagtttttt cgtaatcttt actgaaggtt gcgttgttga
    22621 gtgctttgac ctccttgcca atagtaacga tcaaatcacg tttcggttcg aggtggcgta
    22681 aacgtaactc tttaagatta tctgacagct cccaggcacc atccactttg ccgctttttt
    22741 tatcgacgac atgaataacg cgtgagaaat cctgatccgg gtcgagaggg atggagaacg
    22801 tcagcaccag cgtggctgca ccatcaagtt ggacttcaga gagatccagc aatgtcagca
    22861 ccttaccggc actctgttgg gccagttttt gtctctccgg cacggagagt tttgctgaac
    22921 tcgcgttttc tgaagaggcc gctttagtaa cttcagaagg cgcatctttt ttcaccgctg
    22981 ttggcgcgtt atcgttgttg tcgcaccctg ccagcgccag cattagcatg caggcggcta
    23041 cgcgtaactt tttcattttt catccctgcc acaatggccc gttagcaacg tcgaataatt
    23101 attatgcgtg agaatttacg ttatgtaatt cagtatcacg cccatacaat ccgaatttta
    23161 gtgagaattg tggctcagac agccaaatca gcctctttcg cttgtcgctt ttcactaaac
    23221 atccgacaat ttagcctaac cccggcaaaa atggagatgc ctatgtccac gacatggttt
    23281 gtaggagccg actggctcgc cgaacatatt gatgacccgg aaattcagat tattgatgcc
    23341 cgcatggcgt cgcctggaca ggaagatcgt aacgttgctc aggagtatct gaatggacat
    23401 attcccggcg cagtgttttt tgatatcgaa gcgctttctg atcacacttc cccgcttccg
    23461 cacatgctgc cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag
    23521 gataagcacc tgattgtcta tgacgaaggt aatcttttct cagccccacg agcatggtgg
    23581 atgctgcgca cctttggtgt agagaaagtg tcgattctgg ggggtggact tgcaggctgg
    23641 cagcgcgatg atctgctgtt agaagaaggt gcagtagagc tgccggaagg agagtttaac
    23701 gccgcgttta atcctgaagc cgtggtgaaa gtaaccgatg tattattggc aagccatgaa
    23761 aatacggcgc aaattattga tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa
    23821 cctcgcccag gtttacgtcg cggacatatt cccggagcac tgaatgttcc gtggacggaa
    23881 ctggtgcgcg aaggcgaact aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc
    23941 ggcgtcagct acgacaaacc aattatcgtc agctgcggct ctggtgtaac ggcagccgtg
    24001 gttttgttag cactcgcgac gctggatgtg ccaaacgtga aactgtacga cggcgcatgg
    24061 agtgaatggg gcgcgcgggc agatttaccg gttgagccag tgaaataagc tttatacagg
    24121 caataaaaaa ccgccgaatt tggcggtttt ttattgctgg tctggttcgc ggcctttcca
    24181 gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa
    24241 ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt atccactgcg
    24301 gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt
    24361 agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat
    24421 gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta cttattgttt
    24481 ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac aatttactgg
    24541 tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg gcggcatatt
    24601 ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa
    24661 tggcacaata tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca
    24721 aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg tcgcctgaat
    24781 aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc
    24841 gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt
    24901 ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc
    24961 accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa tcaacaaact
    25021 gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga agagcgtttc
    25081 gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc
    25141 aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc
    25201 tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc cctgcgccgc
    25261 cgtaccaggc acccagacag tggattccag tagagtacgg aaaaaggccg ggcggtgcgc
    25321 cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacataag
    25381 aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct tatcaggcct
    25441 acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag gcgtttacgc
    25501 cgcatccgga atattagttt acgccgttaa cagattagct atcgtgcgca caccaagtcc
    25561 cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat
    25621 atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg ccgccgtgct
    25681 cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt tagacggcag
    25741 ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct cttgtgaggc
    25801 actcgccagc aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt gataatcatt
    25861 acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca tttccggttt
    25921 ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg catcagtgtt
    25981 catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct tgaacgcatt
    26041 gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt tcagtccgcg
    26101 cgtaatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat
    26161 cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag tgataccttt
    26221 acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt aatccagcgc
    26281 cagcaatacc ggagaacgtt ctgaaccgcg cccgacggtg tgcagcccca tataaccttg
    26341 ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgaccgcccg cgacgttgct
    26401 gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg ccggtgcgtt
    26461 gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct ggcgctgggc
    26521 atcgtccaga tccggccact ccactttacg cgtgcctttc ggggctttgt aaccttgcca
    26581 gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat gcttgatgcc
    26641 cagaccgtca atcttgcgcg ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa
    26701 atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc agcgcgcgtc
    26761 ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta tccttctttg
    26821 taaaagggcc gccagttggc agccccagga tttttccgct aaatgattcg cgttgcagga
    26881 aggcggcaag tgagtgaagc ccccaggagc atagataact atgtgactgg ggtgaacgag
    26941 cgcagccaac gcatctgcgg cgtgaagcat gacgcggaaa ttattcggcc tcgtccagcc
    27001 agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat
    27061 cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc ggatcaagat
    27121 cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccat ttaagtccca
    27181 tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga tttcgaccac
    27241 taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct ccagtcccca
    27301 ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagcgagt caaaaccttc
    27361 acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt gttcaatctc
    27421 gataccgtta cgcagagctg cgtcgagaat ggtttcaccg ctattagctt ccagaacagc
    27481 gccatcaggg cagagatcct gatgaggcaa aataacaatc tttggcatat taaacctcgt
    27541 ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga gcggcgaaat
    27601 cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaacg tcatcgccct
    27661 gcgccacttc actcaggtga gcggcagcat cgtcaatgac ctgacgttct gcggcgctta
    27721 acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc gccgcttcta
    27781 ctttttgttc tgccagcatt cgggctttta cgtcctgctc ggcatagctc attgagtctt
    27841 tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc tgaatagacg
    27901 cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct
    27961 ggaacgtcac gcgaatatgc gcaccaccag ccggtagcgc cggaatacca cgcagcgcaa
    28021 aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt acatggatag
    28081 acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg ggcgcgggcc accggaatcg
    28141 tggtattacg cgggatcact ttctccacca ggccgcccat cgtttcgagg cccagcgaca
    28201 gcgggatcac atcaagcagc agcatttcgc tgtccggctt gttacccacc agaatatccg
    28261 cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc agcggtggac
    28321 gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac acgagtagaa ccgccaacca
    28381 tcaccacttc cagcacttca tcagcttcta cgcccgcgtc tttcagcgca cgacgacaag
    28441 ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca cggctgattt
    28501 cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc gcgattttgg
    28561 ctgcaatggc ggcatccagc agttcacgct gaacgcggtt atcgctacga tcaggaatgt
    28621 ccgcctgctc gcgaatgtaa tccgccagca gatgatcgaa atcatcgccg ccgagcgcgg
    28681 aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga atggaaatat
    28741 caaacgtccc gccaccgagg tcataaacgg cgatcacgcc ttcctgaccg gaatccagcc
    28801 cgtaggcgat agccgcagcg gtcggttcgt taagtaagcg caggacgtga aggcccgcca
    28861 gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga
    28921 taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc agtgctttga
    28981 ggatgtccgc agaaacgcgc accgggttca gcagccccgc cgccgtttca atcatcggta
    29041 ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc tggatatcag
    29101 ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggta tcgagcgctg
    29161 cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga acaacagatg
    29221 gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg
    29281 ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc tgatgcggcg
    29341 cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc cagaaattaa
    29401 aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc gagaaaacgc
    29461 agcttacgca cggtatccgc cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc
    29521 aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc cagccgcgct
    29581 tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg ttccatcagg
    29641 aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg taaagaaagc
    29701 aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt aatggttgca
    29761 gattgctgta cggcggcgag ttgttccgcc tggcttccgc tggcgaattt atcaggatga
    29821 tactgacgtt gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag ttgataacgg
    29881 gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg atccgccgcg
    29941 ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg ggttatcggt
    30001 atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg ttcgggttgg
    30061 tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta ccgtccagaa
    30121 attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac acgatgtctt
    30181 ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat ccggaggttc
    30241 tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta tttactcgcg
    30301 ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta ttttgcttca
    30361 cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag aatagaacag
    30421 tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt gatcgcctgc
    30481 gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga agcgatagca
    30541 gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc gttgacttta
    30601 atctgcaact tcatcacgtc gccacaggcc ggtgccccca ccatgccgct gccgacgttc
    30661 tcgtcgttgt tgtcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt
    30721 tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg
    30781 atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga
    30841 cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa
    30901 cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc
    30961 agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga aactgcgagg
    31021 tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg
    31081 ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg
    31141 ttccatagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt
    31201 gcgatgcgat aggcttcgcc catgccgacg atctggtgaa caggcagagt gccggaacgc
    31261 ataccgcgtt cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacgcg cggtttacga
    31321 cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga gaaagacatc
    31381 aggtcaactt tcaactggct caggtcgata ggcagtttac ccacgctctg ggttgcatca
    31441 acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc
    31501 acgccgattt cgttatttac gtgcatgatg gacacgagga tggtgtcgtc acgcatcgct
    31561 gcttcaagtt ctttcaggtc gataatgccg ttacgctgcg gtgccaggta ggtgacttca
    31621 aaaccttcgc gctccagctg acggcaggta tccagtaccg ctttgtgttc ggttttgctg
    31681 gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg
    31741 tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg
    31801 gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa
    31861 cgggaggccg ggttaccaaa ggttccgtcc atcgtcataa actgcatcat tttctcggca
    31921 acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct
    31981 ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga tgtacgaccg
    32041 tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac
    32101 ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca
    32161 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt gttgagaaaa
    32221 ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc
    32281 tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatt
    32341 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
    32401 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
    32461 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
    32521 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
    32581 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
    32641 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
    32701 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtccttc
    32761 cagaccatgg gcgggttgag ggcaattatt cgcattagcg ctggcctctt cccctcaccc
    32821 taaccctctc cccgaagggg cgaggggacc gtccgtgcat ttatctggct tagccctcag
    32881 gccttattac ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat
    32941 tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc
    33001 ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa
    33061 cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccgttt
    33121 tcctgagttg ccagccaggc catgcgcact tcataggcaa taacctgaac cgccatcgcc
    33181 aggttcagcg agctgtattc cgggttagcc gcaatcgcga catgataatg gcatttctgc
    33241 aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac aggggtattt
    33301 gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc
    33361 gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc
    33421 gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgctgccag ggcaatcgcc
    33481 tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc
    33541 atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga
    33601 atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg
    33661 aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag
    33721 tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt
    33781 aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt
    33841 aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt
    33901 aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat
    33961 caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg
    34021 ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta
    34081 tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc acagctgaac
    34141 ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc
    34201 ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg caaactgttc
    34261 aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct
    34321 gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc
    34381 gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat
    34441 atgctgaccg gtaacatcgt tgctggtaac ccacgtgttg tgaaagccat gctggcgaac
    34501 atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt gatgtcactc
    34561 acccgccctc gcctttcagg cgctattctg aaatacttcc tcaccgcttt actttctttc
    34621 gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca aggaaaggtt
    34681 atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt tgttgttgcc
    34741 ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa
    34801 atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact
    34861 cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc catcgcaacc
    34921 atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg
    34981 ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa
    35041 ggcacgccgt cccaagccgg attgctggac gatacgcaaa gtgccatcaa tgtggtgcgc
    35101 catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat tggcggggcg
    35161 aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt gatcctcgac
    35221 tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag tggctactta
    35281 cttgatgaga gttacagcgg cgaaaattat atcgccagcg tcagcccgat cccgctttta
    35341 ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc
    35401 ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt
    35461 tccgatcgtc acggcgatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg
    35521 aatccacaga actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga
    35581 catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac gcttgccaac
    35641 atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc
    35701 aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc
    35761 ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac
    35821 cacggcaact ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag
    35881 cgaaacagga caggagatcc tgcgctatca tcaactcaca ctgacggctg gttatgacgg
    35941 tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg
    36001 acgtggtttc cgtctgtgtc catcatttat taccagccat ttcacccccg ccctgaagag
    36061 tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt
    36121 agtgaatctc tgctcccgac gactgcaaaa acgctttgaa acgcgcgata ttcatttcct
    36181 gtgtctgtat ctgcaatatt gtttgctgca acaccacgct ggaattacgc ctcagtttaa
    36241 tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg
    36301 acgccactgg cagcgtcggg cgctccagcc tgtaccgcct gatgagccac tgtttatggc
    36361 attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc agcgagacag
    36421 acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg
    36481 tttttatgat gaacaggggt tatgcgatca gctttatacc cacctcgccc aggcgttaaa
    36541 tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta
    36601 cccacgcctg gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt
    36661 ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca
    36721 ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga
    36781 agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat
    36841 gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc
    36901 ttataccatg ccattaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac
    36961 aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg
    37021 ggcgcaaaaa cattgccggc agcgccacca gcgccattac ccagaacacg ccgtggccca
    37081 gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga
    37141 cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgagca gcaatataac
    37201 gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc accactatca
    37261 accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg
    37321 agatcaacag catatcgcgt gcactacaac ggcggaaaag tttattactc agcgcaaaga
    37381 taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc
    37441 cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata
    37501 aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac gctgaccaac
    37561 cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg atgagaaagc
    37621 cgagcagcat ggatgccacg cccaacgtca acagcgcgag gatcacccga taatcaaaca
    37681 tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc
    37741 gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca
    37801 acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta
    37861 cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc agcacgcgca
    37921 aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa
    37981 aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc
    38041 ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat
    38101 agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt
    38161 tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt
    38221 cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gagatccgcg acgcatgttg
    38281 caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga
    38341 aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag
    38401 gcacatttcc ggcaatggga cgaaaaacaa cctgtccggt attaaaatta ttcatataac
    38461 cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg
    38521 ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg
    38581 gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg
    38641 cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacaca accactaatg
    38701 gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc
    38761 cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca
    38821 gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcagtaaat
    38881 tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc
    38941 gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac
    39001 attctcccgc cgctgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga
    39061 cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt
    39121 ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta
    39181 gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt
    39241 cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg tcaatacatc
    39301 ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat
    39361 tgacacccaa aatggtcggg tcactccgcg tatttatacc gacccggata tttaccaact
    39421 ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa gccagatccc
    39481 aaaacccggt gatttcttta acacctacat gggagaagat gcggttgtcg tagtgcgtca
    39541 gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag
    39601 ttatgcagat tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct ggtcttatgg
    39661 cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg
    39721 taaatcccac tggggactaa acgaagttcc ttgtgtggag agttataaag ggctgatttt
    39781 tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca ttgcctggta
    39841 tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa
    39901 gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca
    39961 tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga
    40021 taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct
    40081 gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa
    40141 tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca
    40201 acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat tccccacgct
    40261 ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc ctgatcaagt
    40321 tgaagtgtgg gcgttctgta ttactgacaa agccacctcc gatgaagtta aagccgcttt
    40381 tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga
    40441 gaactggtgt gaaatccaga aattgctgaa aggtcaccgc gcccgcaaca gcaaactgtg
    40501 tctggaaatg gggcttggtc aggaaaagcg tcgcgacgac ggcattccag gcattactaa
    40561 ctatatcttc tcagaaacgg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag
    40621 tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa
    40681 atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct ctttcacgaa
    40741 gccagcttac tggacgactg gaaatttcgt gactggctgg cgcagctcga cgaagagatt
    40801 cgttacacca tgcgcaccac agttaacgcg caaacacgcg accgccgcaa aggcgtccag
    40861 ccaccgacaa cctggatttt taatgacacc aaagaccagc tggagcggcg aatcgcccgt
    40921 ctggaaacgg gcatggcatg ggcagaagag ccgccgtcac gcacccgcca cttaatcagc
    40981 aactgccaga taagcgaaac cgacatccca aacgtatttg ctgttcgggt gaattatctg
    41041 ctttatcggg cacaaaaaga gcgcgatgaa acgttctatg tcgggactcg ttttgacaaa
    41101 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa
    41161 gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc
    41221 ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc gtcatcgccc
    41281 tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt
    41341 caatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca
    41401 gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt
    41461 atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag
    41521 cgatttgcat aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct
    41581 ggtcgagcga tttatcgaag aaggcgcgca ggttgccacg ctggaactgt cggcggcaaa
    41641 agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac
    41701 ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga
    41761 ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc
    41821 agagacgctc gaaaccggct tccacgagct gtttaacgtc aatgttctcg gttacctgct
    41881 gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact
    41941 gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccgcca gtaaacatgc
    42001 cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg
    42061 cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga
    42121 aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt
    42181 tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa
    42241 tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg
    42301 ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat
    42361 tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac
    42421 cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc
    42481 gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg
    42541 gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac
    42601 acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaac
    42661 cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct
    42721 acgccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt
    42781 gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagatg
    42841 taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt
    42901 gcaacgctat cttttacagc gccaccagca ggctggtgtg cgcattctgc tcaataatgc
    42961 cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct
    43021 tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac tggctcgcga
    43081 ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca cctgcgatcc
    43141 cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg cactacaccg
    43201 ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg caatgttagg
    43261 gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca gtgataactt
    43321 acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc cggaaactca
    43381 gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca
    43441 ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa
    43501 actgctgata gatgagaaca tcgcgcttaa atcactgtaa ccaggataat tagcgaatat
    43561 ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat cacattatct
    43621 tgcaaaggga ttggttatga acacactacg ttattttgat tttggagctg cccgccccgt
    43681 tttgttatta attgcccgta tcgccgtggt cttaattttc attatttttg gttttcccaa
    43741 aatgatgggc tttgacggta cggtccaata tatggcctcg ttgggcgcgc caatgccgat
    43801 gctggcagcg attattgcgg tagttatgga agtgcccgcc gcgatattaa tcgtgcttgg
    43861 ctttttcacc cgtccgctgg cggtgctgtt tattttctac acgctgggta cggcggtgat
    43921 tggtcaccat tactgggata tgaccggcga tgcggttggg ccaaatatga ttaatttctg
    43981 gaagaatgtc agtatcgctg gcgcattcct gctgctggca attaccgggc cgggggcaat
    44041 ttctctcgat cggcgttagg aaagatgccg gatgcggcgt gaacgcctta tccggcattt
    44101 aataaattac agccactcta cccgcaacga catctctgaa gttgttgatt cccctggtgc
    44161 tagcgcaatg agatcacccc cttccgggcg atgatgatca tccggcgcat ggctcatcgg
    44221 ttccagacag aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg aaacaaagat
    44281 gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt cctgcggctg
    44341 ctcgatgcgg gcctgaccat tccatccggc aaaaccattg ttcacccact ggcgcggcaa
    44401 cggcgcgggc tggttaaaat ccagttcctg cggtagctgc tcgcaaaact cacccgccag
    44461 ccactgctcc cgctccagcc agtaaccgct cgcctgtgcc tgaatccgcg tttgcggcga
    44521 caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc cttgattggt
    44581 gacagagagc gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac ttacccgata
    44641 gtgatagaca ccgctgcgat gttcatacac cagacacaaa ctatcatcgc tgtgcgagac
    44701 acattgccac tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc actcaacatt
    44761 cggttgcagt tgatactcac gcccctgcca gacaaaacga ttgccgctca cccggtttgc
    44821 aaatggcacc agcggaaaac atgaggcatc ggttgccaca ccgctttttt taccaggacg
    44881 taagagcggc gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt cagaaacgtc
    44941 cagctttagc gacccgtggg ataaggtata gatggtcatc cgctaactcc ttaatccggg
    45001 aagttaatca caactttccc gcatttgccg ctcgccatca gcgcataggc atcacctgcc
    45061 tgttccagcg agaagcgatg ggtaatggcg ttacgcggcc acagcttcca gtccgtcaga
    45121 tcatgggcgc atttttccat atggaacaga ctggtcaccc aggagccgat aatccgccgt
    45181 tgatggtgca tcagatcggc gctgacctcg aattccactt ttccggtttc accaatgtaa
    45241 accacccgtc cccagtcagc ggtggattgc agtgccagca agcgacctgc ggcattaccg
    45301 gaacaatcga gcgcaacatc cgcgccaccg tgggtgagtt cggcgataat ctgcggcaga
    45361 ccttcggtgg ttgctaaata gccgtgatcc atcaccccta actgttttgc catcgccaga
    45421 cgttccggca gcatatcaac gccgatgatc cgttttgcac cgcgaccttt cgccagcatc
    45481 atcgccatca tgccgaccgg ccccagaccg accaccagca ctttatcgct gccggaaact
    45541 tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc aactgataaa cgcaccatct
    45601 tcgtagctca gcgcatccgg caggaggatc agatcttttt cttccgccag cagatattcg
    45661 gcatgaccgc cgtcacgctg ccagccgtaa gctgcttttc ctttgccagt acaagagata
    45721 ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata caccagcacg
    45781 cgatcaccct ctttaaaatg gcgacagcct tgccccatcg ccacaatctg cccgcacggt
    45841 tcatgaccgt tgataaatcc ctggtataac ggtttatcgg gtgccgccgc cgtggcacgg
    45901 tgttgatgat agatatagtg gacatcgctt ccgcaaatcc cggaggattt cattttgatc
    45961 agtacctggt taatccccgg cgtcggcacc gcaacttccc gcagatcgac ggtcgaattt
    46021 cctggtaaat aagctgccag catcgttttc ataaatccct cattaacaat acgattaatt
    46081 ttcatccctg ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct ttactgctac
    46141 gctgggtcag caagatattc gccagcaccg ccaccacgat gatgacgccg cgcaccacct
    46201 gctggaaaaa ggagttaatc ccgagcagca ccagaccgtt accgattagc gtaatcacca
    46261 gcacaccaag caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc gtaccgccga
    46321 ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg ttaccagaac
    46381 cgaggcgcgc cgccaacaaa atgccggtca ccgccgctaa taatcccgaa agggtaaaga
    46441 taagaatgcg cacccgacga acgttgatgc cgcacaactg cgccgccgta gcattaccgc
    46501 caacggcaaa aaccgagcgc ccgaaggcgg ttttgcggct gatgaacaca aacagcgcaa
    46561 acaacacaat catgatcagc gcggataccg gcacaccgag aaattgtccg cccagccagt
    46621 ccagcacctc gttttcgtca atcggcactg gcagtgcgtt cgtcataaac agccccattc
    46681 cgcgcagggc gctccacagc cccagtgtgg caacgaaact tggtacgtta aacacgccgc
    46741 gcagcacccc ggccagcgtt cccatcagcg cgcctaacag caacaccagc agacacgcca
    46801 ccgccagcgg aacttcaaat tgcagcaaaa atgccaggca caccgagaca aaagccacca
    46861 tcggcccaac gctgacatca atctcaccgg agataataat cagcgtcatc gcccaggcgg
    46921 caatcccaat ggtggcggca tcgcgcagca cgttcatctg gttattcaat gagataaagc
    46981 caggcgcgtt cagggagaag accagataaa gaatggcaat caccaccagc aaaccgatct
    47041 cattaatatg gcgactgaca aattgtttga gcgagacgct cttgccctgc ggcaatggta
    47101 atgacgaagc agacatcttc aggttcctcg gtaaaattca tcagtgcaca gacagaatgg
    47161 cggacatcag ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac gtgccgtgct
    47221 gtaataacag gatgcggtca cacaccagcg gcaactcctc cacttcactg gagataaaca
    47281 ccacgctttt tccttcggca gccagctcac ggacaatacg gtaaatctgc tgtttggctt
    47341 cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc
    47401 aacgaccgat cactactttt tgctgattgc cgccagaaag cgtgccgatg ggcgtttcgc
    47461 tactggcggc cttgaccgtc atccgctgca tcacttcttc ggtcaggcgg cggatggtgg
    47521 accattgcag cactccgttg gcgctgattt tttgccgatt ggtcagcact gtattttcgt
    47581 caacgcccaa ccagggaatg atccccgctt ctttgcggtt ttctggcgta tagccaatgc
    47641 cgcgtttcag catgtcgccg taatcggggc gcgtgatttt ctcgccgttg ataacaattt
    47701 agccctgttc atactcctcc agcccaacaa tcgccttcag caattcactg cgccctgccc
    47761 ccagcagacc agcaatgccg agcacttcgc cacgacgtag cgtaaaactg atatcctcca
    47821 gcttgggctt atggcgtaac gcacggactt ccagtacggc ctgatccaca atttcctgag
    47881 gggcaaccgg cgcaatatca acgtgatcgc gcccgagcat cagcgacaca atatgatgcg
    47941 tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc atcgcgcata acggtggcac
    48001 aggaggcaat gcggcgaatt tcttccatcc ggtggctgac ataaattacc gccacgccca
    48061 gtgccgacat ctttttcacc gcgctgatca ccagttcaac ttccgcactg gcaagcgaac
    48121 tggtaggttc atcaagaatg accacgcgcg gctcgccctt catcacccgc gcaatttcca
    48181 ccagctgctt ttgcgccggg cttagtgttg aaacaagttg ttcaggacta acgtcaacgc
    48241 ccagcgcctg taagcaacgt tgggcatcct gcgccatttg caggtaatca atcatgccgt
    48301 tgcggcgggg ccactgaccg aggcagaggt tttccgccac cgtcagccct tccaccagac
    48361 ttaactcctg ataaaccgcg cgtaccccca gttcagcggc acggcgagtc agcgtagctt
    48421 cgtcgccttc cagtcgcgtc tcgccaatcc agatatcacc gctatccgga cgttcgctac
    48481 cggtaagcat tcgaatgagg gtcgatttgc ccgcgccgtt tttgcctaac agcgcacgaa
    48541 cttcgccttt attgagcgtg aagttaacgt tatccagcgc aacgacgccg gggtaacgct
    48601 tatttcctgc caccactttt gctaccggga ctgcctctgt tgccgtgaac atagggacct
    48661 ctgcgaatca gcgattaggg cagaccatca acgtgcgttg ccagccactg tttaccgtct
    48721 tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta
    48781 ttgataacct tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc
    48841 accaccgctt tcaacacctg attgttttcc agctcctgag caatttcagt ggtcatatcc
    48901 gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg
    48961 agtgtcgcac cgcccgattc ccccataatg gcgttgagat ccggcgtgga gataattagt
    49021 ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctgattagc gacaatttgc
    49081 gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg cacacaaact
    49141 tcaaaggctt cgcaattgat gacggcaatt ttcggctggt caattttatt ggcaataaaa
    49201 taatcggcgg cagcgttacc cagtttttta ccaaattcca gcggatcgcc gaccagatac
    49261 gccgagacat atttatcgac ccccttttga ttaatacagg tgttgtagca aatcaccgga
    49321 atacccgctt cactggcgcg acgaacggta ctgctactgc cattttcaga cactgccgat
    49381 aaaataatgg catcgacatt acgcgccacg agggtatcaa caaaggtact ttctttcgaa
    49441 atatcaccct gggcgttagt ttcaattaac tgtacctgta ctgaagaatc ttttgccgca
    49501 tcctgaacgc cctgacgcac tccagcgtaa tatccctggg tatcaaggta tattgcacca
    49561 atggtcattt ctttatccgc agccctggca aatagtgcgc tacctaatag cgtagccatt
    49621 aataatagat tacgggttgt tctcattttt ttaggcatag tgcctcctgt agggttttta
    49681 ttaacaacgg cttattctaa ttattttgtg atgagcggca gcgcgtgccg ctcaaatatt
    49741 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacggt ggtgaataat
    49801 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ttcatattgc
    49861 gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc
    49921 tgctgttgta aatcgccata cagcgacaac aggtcaggtt gtgaaacagc aaagaaatcc
    49981 gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc
    50041 tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca
    50101 accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga
    50161 cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
    50221 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
    50281 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct gtctggcatc aagatgctgc
    50341 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
    50401 cacgggtgga atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga
    50461 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
    50521 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc cagcaaatcc
    50581 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
    50641 gcggccagtt cataatcgtg ttgcttattc cacgcatgga tcgccaaccc gcgccagcca
    50701 tcggcaaact ccggcgacat ctctacgcaa ctttgccata aagtaatggc tttgttgtag
    50761 ctgcgcttgt tgtagtagaa gcaggccagt aaatgacggg caaaccagca ctcttcaata
    50821 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
    50881 tcaatggctt ttgcgaccag ttcgccgcgt tcggctttcg gcagcaagct ggcttgtaga
    50941 taaagcgata acgtgcgctg acagtccagt gcgttcagca tctctgtcgc cagagtgggc
    51001 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
    51061 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
    51121 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc
    51181 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
    51241 caggcacgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc
    51301 gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc
    51361 cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
    51421 aaactggcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagcgcctga
    51481 gtggcatacg ccaccgcttg cgggaaatct gcgcggttat attccagcat cgccagcgcc
    51541 aggttacagc ggtaatccag cggatccagc accacgccgc gcaggtagta atcgaacggt
    51601 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
    51661 ctggtaatgt cttgtgctgc gagtggtgct ttggcgacgt ccggcaacgg caactcttgc
    51721 ggctgatgtt cctgataact caggacaata ttgccgtcgg catcggagag ctcaatggtc
    51781 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
    51841 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg
    51901 ttcaacggag agatggcata cagcccccac tcaatccccc gctcactacg ctggagtttt
    51961 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
    52021 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcaggctg gttatcggca
    52081 aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact cttgtcccac
    52141 gcctggccga attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg
    52201 ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac
    52261 tgcgattttt cggccatata tgaggttgga acaggcacat ttttatagcg agaaatgtcc
    52321 actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
    52381 gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct
    52441 tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg
    52501 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
    52561 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct
    52621 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc
    52681 cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta
    52741 atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc
    52801 cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
    52861 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
    52921 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
    52981 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
    53041 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
    53101 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
    53161 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
    53221 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
    53281 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat atcgacgatg
    53341 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
    53401 cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
    53461 gtccgaaagg cgagtttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
    53521 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
    53581 tggcgctggc aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc
    53641 aagcgccgtg gacaacgccg gtggaggtga aatatctgct ggaagagaag ctcggcattc
    53701 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt
    53761 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
    53821 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acagattggt cacaccatcg
    53881 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
    53941 cgttaagtgc tttaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
    54001 agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac
    54061 cgtggatcac cagctgggtt gaccgcagtg ccaatgccat tggtttgagt ctgtataact
    54121 tcctcaacat cctcaatatt aatcagatct ggttgtacgg tcgcagttgt gcctttggtg
    54181 agaactggct taatactatt attcgccaga cgggatttaa cccgttcgac cgcgacgaag
    54241 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
    54301 tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg
    54361 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
    54421 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
    54481 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
    54541 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
    54601 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
    54661 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
    54721 tcagtgccgc cggaaaccac tttgtagccg cgttccagga acacttctac catcgcttta
    54781 gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
    54841 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
    54901 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
    54961 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
    55021 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
    55081 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc ggaatatgca
    55141 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
    55201 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
    55261 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
    55321 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
    55381 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
    55441 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
    55501 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
    55561 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
    55621 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
    55681 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
    55741 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
    55801 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
    55861 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
    55921 acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag atgcaaaaaa
    55981 ggaagaccat atgcttgacg ctcaaaccat cgctacagta aaagccacca tccctttact
    56041 ggtggaaacg gggccaaagt taaccgccca tttctacgac cgtatgttta ctcataaccc
    56101 agaactcaaa gaaattttta acatgagtaa ccagcgtaat ggcgatcaac gtgaagcact
    56161 gtttaacgct attgccgcct acgccagtaa tattgaaaac ctgcctgcgc tgctgccagc
    56221 ggtagaaaaa atcgcgcaga agcacaccag cttccagatc aaaccggaac agtacaacat
    56281 cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc agcccggggc aggaagtgct
    56341 ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta tttatcaatc gcgaggcgga
    56401 aatctataac gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg atttccgcat
    56461 tgtggctaaa acaccgcgca gcgcgcttat caccagcttc gaactggagc cggtcgacgg
    56521 tggcgcagtg gcagaatacc gtccggggca atatctcggc gtctggctga agccggaagg
    56581 tttcccgcat caggaaattc gtcagtactc tttgactcgc aaaccggatg gcaaaggcta
    56641 tcgtattgcg gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc acaatcacgc
    56701 caatgttggc gatgtcgtga aactggtcgc tccggcaggt gatttcttta tggctgtcgc
    56761 agatgacaca ccagtgacgt taatctctgc cggtgttggt caaacgccaa tgctggcaat
    56821 gctcgacacg ctggcaaaag caggccacac agcacaagtg aactggttcc atgcggcaga
    56881 aaatggcgat gttcacgcct ttgccgatga agttaaggaa ctggggcagt cactgccgcg
    56941 ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta aaggtcagtt
    57001 tgatagcgaa ggtctgatgg atttgagcaa actggaaggt gcgttcagcg atccgacaat
    57061 gcagttctat ctctgcggcc cggttggctt catgcagttt accgcgaaac agttagtgga
    57121 tctgggcgtg aagcaggaaa acattcatta cgaatgcttt ggcccgcata aggtgctgta
    57181 atttgatgtt gccggatgga aacatccggc aacccttgac gcggtttaaa ttgccgcgtc
    57241 gtcctcttca ccggtacgga tgcgaatgac ccgtgccacg tcaaagacga agattttacc
    57301 gtcaccgatt ttgccggttt gcgccgtgcg aataatggta tcgacacagg tatcgacaat
    57361 gtcgtccggt acgacaatct caattttcac tttcggcaga aaatccacca tatactccgc
    57421 gccgcggtac agctcggtat ggcctttctg gcgaccaaag cctttcactt cggtcaccgt
    57481 catgccggta ataccgactt cggccagtgc ttcgcggaca tcgtccagct tgaagggttt
    57541 tataatcgca tcaatctttt tcatgctatt ccttgaaaag gtcgcctgtc ttttgatctg
    57601 ctaaacgtaa cacataacgc caattcattc cttgaaatcg tttgcatcca gctcgtgtcg
    57661 ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc attctcgccg cgtgggtgac
    57721 gttgcctttg gtaatttgca gcagcttacg caaatagttg agttcaaact gattacgtgc
    57781 ctcaacaaag gttggcagcg ccgtgttttc accctccagc gcctgctcta ctaatgcatc
    57841 gctaataacc ggagatgagg tcagcgccac gcactgttca atcacgttga ccaactggcg
    57901 cacattaccc ggccaactag cggtcatcag gcgtttcatc gcatcagtgg agaacgcgcg
    57961 gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca gaagcggaat
    58021 gtcttctgtg cgctctgcca gagccggaat tttcaggctg acaacgttga ggcggtaata
    58081 gagatcttcg cggaattccc cgcgcgccat cgcttttggc agatcacggt gagtggcaga
    58141 aataatccgc acgtcgatat caatatcgcg gttgctgccc agcgggcgca ctttacgctc
    58201 ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc atatcgccaa tttcatcgag
    58261 aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg cgattgctga cagcgccagt
    58321 aaacgcgcca cgcgcatgac caaacagctc cgactccagc aattgctcgg gcaatgcgcc
    58381 acagttaata gcaataaatg gtttgctatt gcgcgggctg gcgttgtgga tagcctgggc
    58441 gaaaatctct ttcccggtgc cgctctggcc gttaatcaaa acgctaacgt ctgattgcgc
    58501 caccagccgc gcctgttcca gcaaacgcag catcagcggg ctgcgggtga caatcgcctc
    58561 gcgccagcgt tcatcggtgg ctggcgcgga ttgttccagc gcatcgtcaa ttgcctgata
    58621 tagcgcatct ttatcgacag gcttggtgag gaaactgaac acgccctgct gtgttgcagc
    58681 aacggcatcg ggaatagaac catgcgcggt aagaataatt actggcattc ccggctgcac
    58741 tttctgaatt tcagcaaaca gctgcatacc gtccatttca tccatccgca ggtcgctgat
    58801 gactaaatct actttttcgc gattcagtac ccgtaatcct tcagcgccac tttccgccgt
    58861 gaccacgctg tagccttcgc tggtcaggcg caggccaagc agtttcagca atcccggatc
    58921 gtcatcgacc aataataaat gcgcaggttt atggctcatc aggagtgacc tcatgggtgg
    58981 atggcgcggg cttttcaccc tcgtgcggcg tatccggcga gaaatttcca gccggtttgc
    59041 gggtcgagag ctggcgttca atatcagtca ggttttccag cttgcgggtg gtgagttcca
    59101 gttgctgctg tagaacgtga tgttgctggc gcaatgtatc cagctcgctg tcgctggcct
    59161 gctggagctt gccgtagcgt tggcgctctt ccgccagttg cagttgcagc gcctgaccgt
    59221 cgcgccagag ttgatacaga gggcgaacct gtgccgggat ctcggtactt agcgcatcaa
    59281 tgcgcgcgac cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg agcaaaatcc
    59341 cttgtttaaa ggtattttgc cagctgccgt cgtcatattg gcgagcttgc tgacgtgact
    59401 gcgcaggcat taaacgatca gcacaatcca tcgcccgcag ccagtaaagc ggattggttt
    59461 cggttgattt accttgcagc gcccagatgt cgctacattc agtagaaaga tagtcagcca
    59521 gttgataaac cggaattttt tcttctgctg gcgtatcaat ggctggctta ttgtgactct
    59581 gcacgcaacc cagcaatgcc agacatggca gccctgccag ccacagccgt cggggcaata
    59641 atcgttgaaa aatgtgtcgc atattcacca gacttaatgc ctatcccagt gggcgtaatt
    59701 attgcaggca gtttggacat ggacagcgcg gagaaaccgg agcgtacacg tcgtacgtga
    59761 ggatttcgag cactgcccgg ggccgaactg gcaaataaaa tagcctgatg ggataggctg
    59821 ttagcattat ttcgtgtttt tcgacgacgg caattcaatg cggaaacaaa cgtcttgccc
    59881 gctctcgtcg accagataca gttccccttg catacggcga atacaatccc tggcaatgct
    59941 taatcccaga ccgctgccct tcaccgcccc ttttcgctgg tggcttcctt gaaaaaaggg
    60001 ttcgaagatc atggcgcgtt cctcttgcgg aatgggtgtg cctgtattga tgacatcaat
    60061 ataaacccgt gcaccatgta agctgctgcg aaggcaaatg ttaccggatt cagccccgta
    60121 gtgcaccgca ttggagtaaa gattatccag tacgctcatc agcagcattg gctccgccag
    60181 gcaagctgtt gctttgagat cgacgtcggt atgcatcatt ttagcccgtg cgggcaggct
    60241 atgagcagaa actactgtct ccaccagcgg tgctaactca acattctcca gttccaccgc
    60301 actgtccgct tgtttacggt tgtaatcaag cagttgttcg atcagttttt gcaaattgcg
    60361 gctgctgcta tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg gcccgacaac
    60421 ctggtcagcc agtaattcag tgccctcgcg catactcgcc agtggcgttt ttaattcatg
    60481 agataaatgt cttaaaaatt gatggcgttg ggattccagc catgacaggc gctcacttaa
    60541 ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt ccactgaacg aaacgctatt
    60601 gcccagagaa cgcccttccc ccagacggtt gatcatgcgc tcgatatttt tcaccggccc
    60661 gataatcatc cgcgtgaaaa gtagcaccat caccagactc accagaaata gcaccagcga
    60721 ttgccaacca aaatattgcc cacgttcggc gatttcacgc tgaagttgct gcccacgaga
    60781 gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa aggcttcaag
    60841 acgcgcggcg gcggcagcat cgggaccact gttgttacac tgaagttggg ccagattgtt
    60901 caaatcctga cgcaatgcct ggtaaagttt atcgtctggc agtacgcctg cgtgggcatc
    60961 gagcatttcg ctgtaacgct tgcgctggct ttgataaacc ttcgcaagcg taggatcgtc
    61021 cagcacgcaa tactgacggt agctacgctc catctccagc gccgcgttgg tcatcgcttc
    61081 actacgccgg gcatcaataa gcgtagtgcg gttaaccagc gccgcctgat cgctaagcgc
    61141 attcaggctt tgccaggctt gccatgccag caccaacagg ggtagtagaa ttagcaaaaa
    61201 tgccagcatt accagttgtc gtaatgagcg gggaaaaaca ggccagcgtt tcaaggtgtt
    61261 actctcgtca gacgcgaata gcctgatgct aaccgagggg aagttcagat acaacaaagc
    61321 cgggaattac ccggctttgt tatggaataa ggcggtgcct aactcgacgt ttcgcccgat
    61381 ggttgatata gctacgctga tatcagaagt tggacggcag gcaccttgtt gtgcgtcatt
    61441 cgaattttat gtagcacgtc ccgaaggggc tgacataagt cggtgaatga gccactgctt
    61501 actattatgc agtaactgtg ccaataaaga aaatagtttg gtaacgtact gattatacgt
    61561 cgctttgagg ggtttatggc ttctccgtta tttgaatgta aatcagccac tgtgtcgcta
    61621 aaaagagaca acttaagata aacttattag ataatattaa aatcaatgag ttaagtgtcg
    61681 ccagaaagcg acacggtaaa ccacccattg tcgtgattta cagacacaaa aaagctcccg
    61741 gagttgggag cttatgatag tggttggtgc ttaatgccgg atgcggcgta aatgccgcat
    61801 ccgggctgca aaaccaatga actgatgact taccccaact gcttacgcgc attgcggaaa
    61861 atgcgcatcc atgggccatc ctcgccccag ttttccggat gccaggagtt gctgacagta
    61921 cggaaaacac gttccgggtg cggcatcata atggtgactc gaccactttc agtcgtaatc
    61981 gacgtaatac cgttcggtga accgttcggg ttagccgggt aggtttcagt gactttgccg
    62041 aagttatcga catagcgcag tgccaccagc cctttgcttt ccagtgccgc cagatgcgcc
    62101 gcatcacgca cttccacgcg cccttcacca tgagagacag caatcggcat ttgcgagccc
    62161 accatcccct gcaacagcag cgacgggctt tgggtcactt caaccaggct gaaacgcgct
    62221 tcaaagcgat cggaggtatt gcgcacaaaa cgtggccaca actcactacc tgggatcagt
    62281 tcgcgcaggt tagacatcat ctggcaaccg ttacataccc ccagcgccag cgtttgcgga
    62341 cggtggaaga aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat tgacttcgcc
    62401 caaccttcac cggcacccag cacatcaccg taggagaaac caccgcacgc gaccagggcg
    62461 tggaaatctt ccaggcccgt gcgtccagtc agcaggtcac tcatatgcac atcgattgca
    62521 tcgaagcctg cacggtggaa agctgccgcc atttcaacat gcgagttcac gccctgctca
    62581 cgcagcacag caactttcgg acgtgcgcca gtggcaatat acggtgctgc cacatcttcg
    62641 ttgatatcga acgacagttt cacattcagg cccggatcgg cgtcgtttga tttcgcctgg
    62701 tgctcctggt cagcacactc cgggttgtca cgcaggcgct gcatctgcca ggtagtttct
    62761 gcccaccaga cacgcaacgt ggtgcggctt tcgctgaata cagtctgccc gttggcggta
    62821 atcacaaaac ggtcaccgga aaccgcctgc cctacataat ggacacaatc agcaagccca
    62881 tgctgtgcca gtacggactc gaccgcttca cggtcagcgg cacgaacctg aatcaccgca
    62941 cccagttctt cgttaaacaa cgccgccagg cgatcgtcgc ccagagaggc aatatccgcg
    63001 ttaatgccgc aatgaccagc aaaggccatt tccgccagcg ttaccagcag gccgccatca
    63061 gagcggtcgt ggtatgcgag cagcttacgc tgtgcaacca gcgcctgaat cgcgtcatag
    63121 aagcctttca gttgcgcgac gttgcgtaca tctgccggtt tgtcgccaag ctgacgataa
    63181 acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc aatcagcagc
    63241 agtgcgttat cttcggtaga aagctgcggc gtgatggtgt gacgtacatc ttccacgcgg
    63301 gcaaatgcgg aaatcaccag cgacagcggc gacgtcattt cgcgctcttc gttaccttcc
    63361 tgccagcggg ttttcatcga catagagtct ttacccaccg ggatcgtaag acccagcgcc
    63421 ggacaaagct cttcacccac ggctttaacg gcttcataca ggcccgcatc ttcaccaggg
    63481 tggcctgccg ccgccatcca gttggcggaa agtttaatgc gtttgatatc accaatctgt
    63541 gttgcggcaa tattggttaa cgcttcaccg accgccagac gggcagaggc ggcgaaatcc
    63601 agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt caccgtagta gctgtcgagg
    63661 ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc acggccccac catctgatcg
    63721 cgcgccacca tgccggttac gctgcggtca ccgatggtca ccaggaaagt tttctccgcc
    63781 acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc ttcacggacc
    63841 agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg cgttttacca
    63901 agcaggacgt ccagcggcag atcgatcggc tgattatcaa aatgacgatc gtgcagagaa
    63961 agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg gcgcgcgttc acgcttacac
    64021 agttcgtcaa acagcggtaa ctgatcggca gcaaccgcca gcacgtagcg ttcctgggat
    64081 tcgttacacc agatttccag cgggctcatg cccggttcgt cgttcagaat atcgcgcagt
    64141 tcgaatttac cgccgcgtcc gccgtcgctc accagttccg gcatcgcgtt agaaagaccg
    64201 cctgcgccga cgtcgtggat aaacagaatt gggttagcat caccaagctg ccagcaacgg
    64261 tcgatcactt cctggcagcg acgctccatc tccgggttgt cgcgctgaac agaagcaaag
    64321 tcgaggtcgg catcagactg accagacgcc atagaagacg ccgcgccgcc gccaagaccg
    64381 atgttcatcg ccgggccgcc gagaacgacc agtttcgcac caacgttgat ctcgcctttt
    64441 tgtacgtgat cggcacgaat gttgccgatc ccgcccgcca gcatgatcgg tttgtgataa
    64501 ccgcgcagct cttcgccgtt gtggctgttc actttttctt cataagtacg gaagtagccg
    64561 ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac cgcccagcgg gccttcggtc
    64621 atgatgtcca gcgcggtgac aatgcgctca ggcttaccga aatcttcttc ccacggctgt
    64681 tcgaagccag gaattcgcag gttggaaacg gagaaaccaa ccaaacccgc tttcggcttc
    64741 gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt cgccgcgccc
    64801 ggccacggag aaatcgccgt cgggtggttg tgagtttcga ctttcatcag aatatgcgcc
    64861 ggttcctgat ggaaatcgta gcggcccgtt tcgtggtcag caaagtagcg gcccacttca
    64921 gaaccttcca ttacggcggc gttatcttta taagcagaga gaacgtgatc tggcgtggtt
    64981 tcgaaagtat ttttgatcat cctgaacagc gatttcggct gttgttcacc gtcgataacc
    65041 caatcggcgt taaaaatctt gtggcggcag tgctcggagt tcgcctgggc gaacatatac
    65101 agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag catcctgcag atagtcaatt
    65161 tcatcttccg ccagagccag gccaagacgc aggttagcgt cgatcagcgc ctgacggccc
    65221 tgccccagca aatcaacgct ggtgaccgga gtcggttgat ggtgggcaaa caactgctct
    65281 gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc agcggtaacc
    65341 tgctgccatt gttcattggt cagcgtaccg gcttctatat agtaagcaac gccgcgctca
    65401 aggcggttta cctgttgtag cccgcagtta tgggcaatat cggtcgcttt cgaagaccag
    65461 ggagagatgg tgccaggacg cggggtcacc agcaggagtt tgccttgcgg ggcgtggctg
    65521 gcgagtgccg gaccatattt cagcaggtgt tcaagttgtg cgtgctcatc atcgtttaac
    65581 ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac cgggagcctg
    65641 gcagcctgaa aacgtgccag cagtttgttg attcggaatg ccgacagtgc aggcgaacca
    65701 cgcagaattt ccatcataag tctctcgtct tctaagcttt cggtgtaccc aaggggggga
    65761 aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg gaaataaaat
    65821 caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc atctggcgca
    65881 ggtttacgca aaatgccgct catttatgag taaacctttc actattatta cgttttttca
    65941 agctgggacg cgcacgacac agagaattaa ctaattgaaa aaattaaaga ttaattatct
    66001 gttcatcggc attctggcac tgctgctcgc ggtcgctctc tggccatcca ttccctggtt
    66061 tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc gtgtgagcac
    66121 cattcatact cccctgactt ataacgaaat caacgggaaa ccttttggcc tggattacga
    66181 actggcgaaa cagtttgccg attacctcgg tgtaaaactg aaagtgaccg tgcggcagaa
    66241 tatcagccag ctgtttgacg acctcgataa tggtaacgcc gacctgctgg cggcaggact
    66301 ggtctataac agtgagcggg taaaaaatta tcagcctggc cctacctatt attccgtgtc
    66361 acaacaactg gtttataaag tgggtcagta tcgcccgcgc acactgggca acctgacggc
    66421 ggagcaactc accgttgcac cgggtcacgt ggtggttaac gatctccaaa ccctgaaaga
    66481 cacaaaattc ccggaattaa gctggaaggt agacgacaaa aaaggctctg cggaattaat
    66541 ggaagatgtc atcgaaggaa aactcgatta caccattgct gattctgtcg ccatcagcct
    66601 gtttcagcgc gttcacccgg agctcgccgt agcgctcgat atcaccgatg aacaaccggt
    66661 gacctggttt agcccgttag atggcgataa taccctttcc gccgccctgc tcgacttctt
    66721 caacgaaatg aatgaagacg gtacgctggc acgcattgaa gagaaatacc tggggcatgg
    66781 cgatgatttt gattacgtcg atacgcgcac atttttacgc gccgtcgatg cggtactgcc
    66841 gcagttaaag cccctgtttg agaaatacgc cgaagaaatt gactggcgtt tgctggccgc
    66901 tattgcttat caggaatcgc actgggatgc acaggccact tcaccgacgg gtgtgcgcgg
    66961 catgatgatg ttaaccaaaa ataccgcgca aagcctcggc attacggatc gtaccgatgc
    67021 cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat atgatgagta aagtgccgga
    67081 aagtgtgccg gagaacgagc ggatctggtt tgccctcgct gcgtacaata tgggctatgc
    67141 gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg acagttgggc
    67201 tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc tattacagca agctgactta
    67261 cggctacgct cgtggacatg aagcctacgc ttatgtcgaa aatattcgta agtatcagat
    67321 tagcctggtg ggttatctgc aagagaaaga gaagcaggct acagaagcgg cgatgcaact
    67381 ggcgcaggat tatccggcgg tatcgcctac ggagttgggc aaagagaaat ttccttttct
    67441 ctcgtttctt tcccagtcgt catcaaacta tttgacccat tctccctctc tgctgttttc
    67501 caggaaaggg agtgaagaga aacaaaatta atccgtcgag gattgcgctt ttttctgcgc
    67561 tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc aacgccgcgc actcatccgc
    67621 cagtattcct tccgtaattt ccactcggtg attcataccc ggatgatgca gcacatccat
    67681 taaagatccc gcagcgccag ttttcgcgtc acgcgcacca aagaccacgc gaccaatgcg
    67741 actgtggatc atcgctccgg cacacattac acatggttca agcgtgacat acaacgtggc
    67801 gtcgatcaga cgataatttt gcatcaccag accaccctgc cgcagggcca tgatttctgc
    67861 atgtgcggtg ggatcatggc gaccaatcgg gcggttccag ccttcgccga ttacccgatt
    67921 gttatgcact aataccgcgc cgaccggcac ttcccgctca tcccaggcac gtttcgccag
    67981 cgtcagcgcg tgacgcatcc agtattcgtg gctaaattcg acttcagaca aaaagaaaac
    68041 tccggttata aaagcgcggc gcattataca cggacgctat gctttactct attccagttg
    68101 ctggagttca ccgcgcgggg ttactcgcca acgatgctga cagaaataaa gcagcggatt
    68161 gtcctgatta ctgtcgctat agccactgta cagccgcagc ggagtgccga ttttgcgctc
    68221 cagttgtgcg accttttcat gtcccagaca acgcatcgtc aatacccaac caccatagcc
    68281 acgctgaatt tggctggcga taagattaac ccgcggcagc cagggcgtat cgaaataaac
    68341 cgcttcaacc agcggctgcg gagagccggt aatcaaccag atatcagcat cggaacttaa
    68401 caggtaggtg gttaatcgct cctgaaccag cggaaaggcg gtaacattgt cgcgaaacca
    68461 gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg ctgtgaccaa aagtgcaccc
    68521 ccacagaagc agactcatcg gccagcgtgc cgcacgacct tttatcaata acgcaatggc
    68581 tataatcggt aacaacggca ggacaagtaa cgcattcagc ggttggcgac gcagtaaata
    68641 gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt caaaaaacac
    68701 cacacgacgc tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc ctgattaatt
    68761 ttcgctacta catagcctaa cagatagatc atcacttttc cggcaacaat cgaccgcatc
    68821 atggctaact ggaattttta attcatggca attagcggca atggaatata aaattcactc
    68881 gcgtgtctct catattgcct gttgtcgcca ctatttcatc gcgaggaaag atatgaacgg
    68941 cttacttcgt atccgtcagc gttaccaggg gcttgcccag agtgataaaa aactggcgga
    69001 ttatctgctg ctacaacctg atacggcgcg ccatttaagc tctcagcaac tggccaacga
    69061 agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct ataaaggttt
    69121 tccggcgctt aagttggcgt tgagtgaagc gctggcaagc cagccggaat cgccctccgt
    69181 gcccattcat aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg aaaaactgat
    69241 taaagaaaat actgccgcta tgtacgcaac gctaaacgtt aatagtgaag agaaactgca
    69301 tgaatgcgta acaatgttgc gctctgcgcg gcggataatt ctgaccggta ttggcgcttc
    69361 gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag attggcttca atgctgccgc
    69421 agtgcgcgat atgcatgcgc tactcgcaac agtacaggcg tcgtcccctg acgatctgtt
    69481 attagccatt tcctacaccg gtgtacgacg cgagttaaac ctggcggcag atgagatgct
    69541 gcgagtgggc ggaaaagtgc tggcgattac cggctttact ccgaatgccc tgcaacagcg
    69601 tgcttctcat tgcctgtata ccattgccga agaacaagcg acaaacagcg cttcaatctc
    69661 tgcttgtcac gctcagggaa tgttaacgga tttgctgttc attgcgctga ttcagcagga
    69721 tctggaactg gcaccagaac gtattcgtca tagtgaagcg ctggtgaaaa aactggtctg
    69781 agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt ttccttatgg
    69841 cgttgttaat cactaaaaaa tgcatcaatt gtgatatgtg tgaacccgaa tgcccgaatg
    69901 aggcgatttc aatgggagat catatctacg agattaacag cgataagtgt accgaatgcg
    69961 tagggcacta cgagacacca acctgccaga aggtgtgccc gatccccaat actattgtga
    70021 aagatccggc gcatgtcgag acagaagaac agttgtggga taaatttgtg ctgatgcacc
    70081 acgcggataa aatttaatat tctactctgg aagtaga
//
LOCUS       DAMZII010000005        68793 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZII010000005 DAMZII010000000
VERSION     DAMZII010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.005"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            28..324
                     /locus_tag="POA77_000280"
     CDS             28..324
                     /locus_tag="POA77_000280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363780.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(482..1129)
                     /gene="ychE"
                     /locus_tag="POA77_000281"
     CDS             complement(482..1129)
                     /gene="ychE"
                     /locus_tag="POA77_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDI6363781.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1606..4281
                     /gene="adhE"
                     /locus_tag="POA77_000282"
                     /gene_synonym="adhC"
     CDS             1606..4281
                     /gene="adhE"
                     /locus_tag="POA77_000282"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI6363782.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4405..5305
                     /locus_tag="POA77_000283"
                     /pseudo
     CDS             4405..5305
                     /locus_tag="POA77_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5587..6204)
                     /gene="tdk"
                     /locus_tag="POA77_000284"
     CDS             complement(5587..6204)
                     /gene="tdk"
                     /locus_tag="POA77_000284"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI6363783.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6810..7223
                     /gene="hns"
                     /locus_tag="POA77_000285"
     CDS             6810..7223
                     /gene="hns"
                     /locus_tag="POA77_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDI6363784.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7367..8275)
                     /gene="galU"
                     /locus_tag="POA77_000286"
     CDS             complement(7367..8275)
                     /gene="galU"
                     /locus_tag="POA77_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI6363785.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8477..9490)
                     /gene="rssB"
                     /locus_tag="POA77_000287"
     CDS             complement(8477..9490)
                     /gene="rssB"
                     /locus_tag="POA77_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDI6363786.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9582..10487)
                     /gene="rssA"
                     /locus_tag="POA77_000288"
     CDS             complement(9582..10487)
                     /gene="rssA"
                     /locus_tag="POA77_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDI6363787.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10600..11058
                     /locus_tag="POA77_000289"
     CDS             10600..11058
                     /locus_tag="POA77_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDI6363788.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            11108..11950
                     /gene="purU"
                     /locus_tag="POA77_000290"
     CDS             11108..11950
                     /gene="purU"
                     /locus_tag="POA77_000290"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDI6363789.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            12110..12194
                     /locus_tag="POA77_000291"
     tRNA            12110..12194
                     /locus_tag="POA77_000291"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12144..12146,aa:Tyr,seq:gta)
     gene            12229..12313
                     /locus_tag="POA77_000292"
     tRNA            12229..12313
                     /locus_tag="POA77_000292"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12263..12265,aa:Tyr,seq:gta)
     gene            12360..12491
                     /locus_tag="POA77_000293"
     ncRNA           12360..12491
                     /ncRNA_class="other"
                     /locus_tag="POA77_000293"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12675..13352)
                     /gene="narI"
                     /locus_tag="POA77_000294"
     CDS             complement(12675..13352)
                     /gene="narI"
                     /locus_tag="POA77_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6363790.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13352..14062)
                     /gene="narJ"
                     /locus_tag="POA77_000295"
     CDS             complement(13352..14062)
                     /gene="narJ"
                     /locus_tag="POA77_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6363791.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(14059..15597)
                     /gene="narH"
                     /locus_tag="POA77_000296"
     CDS             complement(14059..15597)
                     /gene="narH"
                     /locus_tag="POA77_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6363792.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15594..19337)
                     /locus_tag="POA77_000297"
     CDS             complement(15594..19337)
                     /locus_tag="POA77_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI6363793.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(19853..21244)
                     /gene="narK"
                     /locus_tag="POA77_000298"
     CDS             complement(19853..21244)
                     /gene="narK"
                     /locus_tag="POA77_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDI6363794.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            21583..23379
                     /gene="narX"
                     /locus_tag="POA77_000299"
     CDS             21583..23379
                     /gene="narX"
                     /locus_tag="POA77_000299"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDI6363795.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            23372..24022
                     /gene="narL"
                     /locus_tag="POA77_000300"
     CDS             23372..24022
                     /gene="narL"
                     /locus_tag="POA77_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDI6363796.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(24023..25417)
                     /gene="ychO"
                     /locus_tag="POA77_000301"
     CDS             complement(24023..25417)
                     /gene="ychO"
                     /locus_tag="POA77_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDI6363797.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            25602..25955
                     /gene="ychN"
                     /locus_tag="POA77_000302"
     CDS             25602..25955
                     /gene="ychN"
                     /locus_tag="POA77_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDI6363798.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25999..26694)
                     /gene="chaC"
                     /locus_tag="POA77_000303"
     CDS             complement(25999..26694)
                     /gene="chaC"
                     /locus_tag="POA77_000303"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDI6363799.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(26852..27082)
                     /gene="chaB"
                     /locus_tag="POA77_000304"
     CDS             complement(26852..27082)
                     /gene="chaB"
                     /locus_tag="POA77_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDI6363800.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            27352..28452
                     /gene="chaA"
                     /locus_tag="POA77_000305"
     CDS             27352..28452
                     /gene="chaA"
                     /locus_tag="POA77_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDI6363801.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            28857..28964
                     /locus_tag="POA77_000306"
     CDS             28857..28964
                     /locus_tag="POA77_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6363802.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            29393..29500
                     /locus_tag="POA77_000307"
     CDS             29393..29500
                     /locus_tag="POA77_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6363803.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            29928..30035
                     /locus_tag="POA77_000308"
     CDS             29928..30035
                     /locus_tag="POA77_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6363804.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(30184..31038)
                     /gene="kdsA"
                     /locus_tag="POA77_000309"
     CDS             complement(30184..31038)
                     /gene="kdsA"
                     /locus_tag="POA77_000309"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDI6363805.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(31074..31883)
                     /locus_tag="POA77_000310"
     CDS             complement(31074..31883)
                     /locus_tag="POA77_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDI6363806.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(31887..32279)
                     /locus_tag="POA77_000311"
     CDS             complement(31887..32279)
                     /locus_tag="POA77_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDI6363807.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(32276..33109)
                     /gene="prmC"
                     /locus_tag="POA77_000312"
     CDS             complement(32276..33109)
                     /gene="prmC"
                     /locus_tag="POA77_000312"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6363808.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDSVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(33109..34191)
                     /gene="prfA"
                     /locus_tag="POA77_000313"
     CDS             complement(33109..34191)
                     /gene="prfA"
                     /locus_tag="POA77_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI6363809.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(34233..35489)
                     /gene="hemA"
                     /locus_tag="POA77_000314"
     CDS             complement(34233..35489)
                     /gene="hemA"
                     /locus_tag="POA77_000314"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI6363810.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            35703..36326
                     /gene="lolB"
                     /locus_tag="POA77_000315"
     CDS             35703..36326
                     /gene="lolB"
                     /locus_tag="POA77_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDI6363811.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            36326..37177
                     /gene="ispE"
                     /locus_tag="POA77_000316"
     CDS             36326..37177
                     /gene="ispE"
                     /locus_tag="POA77_000316"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI6363812.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            37328..38275
                     /gene="prs"
                     /locus_tag="POA77_000317"
     CDS             37328..38275
                     /gene="prs"
                     /locus_tag="POA77_000317"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI6363813.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            38400..40079
                     /gene="dauA"
                     /locus_tag="POA77_000318"
     CDS             38400..40079
                     /gene="dauA"
                     /locus_tag="POA77_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDI6363814.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(40134..40412)
                     /gene="ychH"
                     /locus_tag="POA77_000319"
     CDS             complement(40134..40412)
                     /gene="ychH"
                     /locus_tag="POA77_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDI6363815.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            40690..41274
                     /gene="pth"
                     /locus_tag="POA77_000320"
     CDS             40690..41274
                     /gene="pth"
                     /locus_tag="POA77_000320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6363816.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            41391..42482
                     /gene="ychF"
                     /locus_tag="POA77_000321"
     CDS             41391..42482
                     /gene="ychF"
                     /locus_tag="POA77_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI6363817.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            43324..46209
                     /locus_tag="POA77_000322"
     CDS             43324..46209
                     /locus_tag="POA77_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HDI6363818.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVIGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNDGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNIGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGKSQLNMYVKTGAIHEFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNTQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(46309..48228)
                     /gene="dhaR"
                     /locus_tag="POA77_000323"
     CDS             complement(46309..48228)
                     /gene="dhaR"
                     /locus_tag="POA77_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDI6363819.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            48456..49526
                     /gene="dhaK"
                     /locus_tag="POA77_000324"
     CDS             48456..49526
                     /gene="dhaK"
                     /locus_tag="POA77_000324"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6363820.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            49537..50169
                     /gene="dhaL"
                     /locus_tag="POA77_000325"
     CDS             49537..50169
                     /gene="dhaL"
                     /locus_tag="POA77_000325"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDI6363821.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            50180..51598
                     /gene="dhaM"
                     /locus_tag="POA77_000326"
     CDS             50180..51598
                     /gene="dhaM"
                     /locus_tag="POA77_000326"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDI6363822.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            51918..53615
                     /gene="treA"
                     /locus_tag="POA77_000327"
     CDS             51918..53615
                     /gene="treA"
                     /locus_tag="POA77_000327"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI6363823.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(53694..54134)
                     /locus_tag="POA77_000328"
     CDS             complement(53694..54134)
                     /locus_tag="POA77_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363824.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(54312..54566)
                     /locus_tag="POA77_000329"
     CDS             complement(54312..54566)
                     /locus_tag="POA77_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6363825.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            54767..55501
                     /locus_tag="POA77_000330"
     CDS             54767..55501
                     /locus_tag="POA77_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDI6363826.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(55503..56114)
                     /gene="emtA"
                     /locus_tag="POA77_000331"
     CDS             complement(55503..56114)
                     /gene="emtA"
                     /locus_tag="POA77_000331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDI6363827.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            56214..57128
                     /gene="ldcA"
                     /locus_tag="POA77_000332"
     CDS             56214..57128
                     /gene="ldcA"
                     /locus_tag="POA77_000332"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDI6363828.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            57223..58959
                     /locus_tag="POA77_000333"
     CDS             57223..58959
                     /locus_tag="POA77_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDI6363829.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(59345..60415)
                     /gene="dadX"
                     /locus_tag="POA77_000334"
     CDS             complement(59345..60415)
                     /gene="dadX"
                     /locus_tag="POA77_000334"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDI6363830.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(60425..61723)
                     /gene="dadA"
                     /locus_tag="POA77_000335"
     CDS             complement(60425..61723)
                     /gene="dadA"
                     /locus_tag="POA77_000335"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDI6363831.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            62053..63585
                     /locus_tag="POA77_000336"
     CDS             62053..63585
                     /locus_tag="POA77_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDI6363832.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YAFGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(63637..64356)
                     /gene="fadR"
                     /locus_tag="POA77_000337"
     CDS             complement(63637..64356)
                     /gene="fadR"
                     /locus_tag="POA77_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDI6363833.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            64578..66119
                     /gene="nhaB"
                     /locus_tag="POA77_000338"
     CDS             64578..66119
                     /gene="nhaB"
                     /locus_tag="POA77_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDI6363834.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            66265..66795
                     /gene="dsbB"
                     /locus_tag="POA77_000339"
     CDS             66265..66795
                     /gene="dsbB"
                     /locus_tag="POA77_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDI6363835.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(66841..68109)
                     /gene="umuC"
                     /locus_tag="POA77_000340"
     CDS             complement(66841..68109)
                     /gene="umuC"
                     /locus_tag="POA77_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDI6363836.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(68109..68528)
                     /locus_tag="POA77_000341"
     CDS             complement(68109..68528)
                     /locus_tag="POA77_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDI6363837.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT        17164 a        17741 c        16894 g        16994 t
ORIGIN      
        1 gtctggcggt tgcccggaaa gtaccaaatg cggttcttac tcgccaatac attttgcaaa
       61 attgttaagc aattctcttt tattgaccgt atcagaatct atagcttgca tccatgcctt
      121 aacaaacatc tttcaattca atcaaataga gattggctaa gcatgtctat cttttctgat
      181 tacagttcca gtagtgaaat gcacaataat ttaacaattg attattattt agcactttcc
      241 tctaccaaag gcagtggaat tactaatatc atttcaatta ttcttcagca agcccaggat
      301 tatgatgtgg cgaaaataac atgatggtga ggagaagcag ttaaggaaat ccttataact
      361 aactgtaatt tgagaagaat atttttacct attccttttg cttttgataa ctgcgcggga
      421 acaaagtaaa aataatgacg aagtaaacgg agcatggcag ctccgtttca tttgaaagga
      481 attaattaag caggccgggg aaaatcccct taataccagt aacgataaat tcaatcccca
      541 atgccatcag caatagcccc ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa
      601 cccgtaccag ccacggtgcc atgcggaaca atccccagca acataaagcg aacaatgcaa
      661 tagccacaaa gaaaccaaac agatagctaa tgctgtgata acgcgtaccc cagacgatgg
      721 tagaactgat cgcccctggc cccgccatca acggcaacgc caatggcacc acaccaatgc
      781 tttcacgtac cgcggtttct gatttttctt gcttgttctg tttatcctcg ccaagcttgc
      841 cgctgatcat cgacatcgct atcgtcacca ccaggatgcc cccggcgata cggaacgaat
      901 caattgatat accaaaaagt tgcagaatcg tgtcgccgag aaaaagcgag atccacaaga
      961 taatggccac agacaggttg gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat
     1021 aactggtcat gctgataaag acgggaataa tccctaccgg gttgaccagc gcaaataacc
     1081 cgatgaaaaa tttgaagtaa acgggaaaat caaaaaaggt ctgaatcacg gttagctccg
     1141 aagcaaaagc cggataatgt tagccataaa taaggttgaa aagacgcgct gacaatacgc
     1201 cttttgacag catttttcac ctcctaacta cttaaaattg ctatcattcg ttattgttat
     1261 ctagttgtgc aaaacatgct aatgtagcca ccaaatcata ctacaattta ttaactgtta
     1321 gctataatgg cgaaaagcga tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc
     1381 acgtaatcag tacccagaag tgagtaatct tgcttacgcc acctggaagt gacgcattag
     1441 agataataac tctaatgttt aaactctttt agtaaatcac agtgagtgtg agcgcgagta
     1501 agcttttgat tttcataggt taagcaaatc atcaccgcac tgactatact ctcgtattcg
     1561 agcagatgat ttactaaaaa agtttaacat tatcaggaga gcattatggc tgttactaat
     1621 gtcgctgaac ttaacgcact cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt
     1681 ttcactcaag agcaagtaga caaaatcttc cgcgccgccg ctctggctgc tgcagatgct
     1741 cgaatcccac tcgcgaaaat ggccgttgcc gaatccggca tgggtatcgt cgaagataaa
     1801 gtgatcaaaa accactttgc ttctgaatat atctacaacg cctataaaga tgaaaaaacc
     1861 tgtggtgttc tgtctgaaga cgacactttt ggtaccatca ctatcgctga accaatcggt
     1921 attatttgcg gtatcgttcc gaccactaac ccgacttcaa ctgctatctt caaatcgctg
     1981 atcagtctga agacccgtaa cgccattatc ttctccccgc acccgcgtgc aaaagatgcc
     2041 accaacaaag cggctgatat cgttctgcag gctgctatcg ctgccggtgc tccgaaagat
     2101 ctgatcggct ggatcgatca accttctgtt gaactgtcta acgcactgat gcaccaccca
     2161 gacatcaacc tgatcctcgc gactggtggt ccgggcatgg ttaaagccgc atacagctcc
     2221 ggtaaaccag ctatcggtgt aggcgcgggc aacactccag ttgttatcga tgaaactgct
     2281 gatatcaaac gtgcagttgc atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc
     2341 tgtgcttctg aacagtctgt tgttgttgtt gactctgttt atgacgctgt acgtgaacgt
     2401 tttgcaaccc acggcggcta tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt
     2461 atcctgaaaa acggtgcgct gaacgcggct atcgttggtc agccagccta taaaattgct
     2521 gaactggcag gcttctctgt accagaaaac accaagattc tgatcggtga agtgaccgtt
     2581 gttgatgaaa gcgaaccgtt cgcacatgaa aaactgtccc cgactctggc aatgtaccgc
     2641 gctaaagatt tcgaagacgc ggtagaaaaa gcagagaaac tggttgctat gggcggtatc
     2701 ggtcatacct cttgcctgta cactgaccag gataaccaac cggctcgcgt ttcttacttc
     2761 ggtcagaaaa tgaaaacggc tcgtatcctg attaacaccc cagcgtctca gggtggtatc
     2821 ggtgacctgt ataacttcaa actcgcacct tccctgactc tgggttgtgg ttcttggggt
     2881 ggtaactcca tctctgaaaa cgttggtccg aaacacctga tcaacaagaa aaccgttgct
     2941 aagcgagctg aaaacatgtt gtggcacaaa cttccgaaat ctatctactt ccgccgtggc
     3001 tccctgccaa tcgcgctgga tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg
     3061 actgaccgct tcctgttcaa caatggttat gctgatcaga tcacttccgt actgaaagca
     3121 gcaggcgttg aaactgaagt cttcttcgaa gtagaagcgg acccgaccct gagcatcgtt
     3181 cgtaaaggtg cagaactggc aaactccttc aaaccagacg tgattatcgc gctgggtggt
     3241 ggttccccga tggacgccgc gaagatcatg tgggttatgt acgaacatcc ggaaactcac
     3301 ttcgaagagc tggcgctgcg ctttatggat atccgtaaac gtatctacaa gttcccgaaa
     3361 atgggcgtga aagcgaaaat gatcgctgtc accaccactt ctggtacagg ttctgaagtc
     3421 actccgtttg cggttgtaac tgacgacgct actggtcaga aatatccgct ggcagactat
     3481 gcgctgactc cggatatggc gattgtcgac gccaacctgg ttatggacat gccgaagtcc
     3541 ctgtgtgctt tcggtggtct ggacgcagta actcacgcca tggaagctta tgtttctgta
     3601 ctggcatctg agttctctga tggtcaggct ctgcaggcac tgaaactgct gaaagaatat
     3661 ctgccagcgt cctaccacga agggtctaaa aatccggtag cgcgtgaacg tgttcacagt
     3721 gcagcgacta tcgcgggtat cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg
     3781 gcgcacaaac tgggttccca gttccatatt ccgcacggtc tggcaaacgc cctgctgatt
     3841 tgtaacgtta ttcgctacaa tgcgaacgac aacccgacca agcagactgc attcagccag
     3901 tatgaccgtc cgcaggctcg ccgtcgttat gctgaaattg ccgaccacct gggtctgagc
     3961 gcaccgggcg accgtactgc tgctaagatc gagaaactgc tggcatggct ggaaacgctg
     4021 aaagctgaac tgggtattcc gaaatctatc cgtgaagctg gcgttcagga agcagacttc
     4081 ctggcgaacg tggataaact gtctgaagat gcattcgatg accagtgcac cggcgctaac
     4141 ccgcgttacc cgctgatctc cgagctgaaa cagattctgc tggataccta ctacggtcgt
     4201 gattatgtag aaggtgaaac tgcagcgaag aaagaagctg ctccggctaa agctgagaaa
     4261 aaagcgaaaa aatccgctta atcagtagcg ctgtctggca acataaacgg ccccttctgg
     4321 gcaatgccga tcagttaagg attagttgac cgatccttaa actgaggcac tataacggct
     4381 tccacaacag ggagccgttt tcttatgcca cttctcaatg atctgctcga tttcagtgac
     4441 catccgctta tgcctccgcc ctctgcacaa ctatttgcag aacaccttcc caccgagtgg
     4501 atacaacact gcctgacgct ttctgctcat gcgaccgttc gccgccgtcg tttaccgggg
     4561 gacatggtta tctggatggt ggtgcaatga gccaattacc gatgttgttc gccgtctgaa
     4621 cctgagcgcg gatggcgaag cggggatgaa cctgctggcc cgcagcgctg tcacccaggc
     4681 gcgtcagcgc gtgggggccg ccccagtgga atggctcttc cgccagaccg cacagacagg
     4741 ggcgcggaac gttacctgaa ggatgactgg cacggcctgc aactttttgc cattgatggc
     4801 gcacagttca ggacacctga ctgataaacc cgagctgcgt gaatattatg ggtctgccaa
     4861 cacatccact aagcggcaga acgcctaccc ggttatgcgt ctggtggcct taatgaacct
     4921 gggaagtcac attctgctga atgccgtgac cgcaccttac cggcagagcg aaactgtgct
     4981 ggcccactcc atgctcgcca ccatcccgga taactctatt acgttgtttg ataaactttt
     5041 ttacagcgaa gacctgctgc tgacgctgaa ccagaagggt tgtaaccgcc actggctact
     5101 gcctgcgtgg aagaatatcg catcagaaat gatagagctg ggaaacacgg cgtcaccagg
     5161 gaccatcccg aaacgactgg agcatcttca gggtgctctg gaagtggtgt tcataacaaa
     5221 acgcccccga ccatcaaggc cgaggtcggt taagatatca aaaacccgtt atcctgtgaa
     5281 acatagtgcc gctcctctta agtgaacggc attgcccttc tggggccgta ttttttgttt
     5341 acccaaagca acttttccat aaaccgacag cattagcctt catcatattt gcgacgatgt
     5401 ataacgccta aacacaggga tattgtactt tacaggtcac aagtcaacgt cggtgcttaa
     5461 gagccctgtg aggcgtatag cggcgttaaa aaactgccga gaagggtata tagcccggaa
     5521 gaagtgcgta aaacgaactg acaggataaa agtgccctgc tcaccctgtc agtaaagaaa
     5581 ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagccga cttgtaacgc
     5641 ctctttatag tgtttacggc atacagaaac gtatcgttca ttaccaccaa ttaccacctg
     5701 ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc ttgctttacg
     5761 gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca gtaagtattg
     5821 gctgccaata aataattcac ctcgaaaatc ggtacgtaaa ccataacaaa gtacgggtat
     5881 atcgagttga tcgacaacct ccgataattc atatacttgt tgtctggtta aaaactggca
     5941 ttcatcaacc agtacgcaat gaattgcctg ctgttcatgt tccgcacgaa tctcatcaaa
     6001 taatgatgaa ttttggttaa ataattttgc aggcgatgac aaacctatac gcgaactgac
     6061 tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc gcatgccgcg
     6121 ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat tcattgcgga
     6181 atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa ataaagttgg
     6241 ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga agtgggtata
     6301 tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca actgcctcgc
     6361 ttgttataag cgggtaaatg actgctggta actattcaca atctttaacc tgttgcgcaa
     6421 gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccatg ctcttattgc
     6481 gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacaca actaatacag
     6541 aagactgaaa ggtcgtcagc ctacgataat ctccccataa aatgtgacat gaatcaggaa
     6601 gttttaacct cacgtgctgc gaaatcatcg gtgtaaatag ggctatatgc cgcgtctttt
     6661 ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca attttgaatt
     6721 ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc tcaacaaacc
     6781 acccccaata taagtttgag attactacaa tgagcgaagc acttaaaatt ctgaacaaca
     6841 tccgtactct tcgtgcgcag gcaagagaat gtacacttga aacgctggaa gaaatgctgg
     6901 aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga aagcgcggct gctgctgaag
     6961 ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat gctgatcgct gacggtattg
     7021 acccgaacga actgctgaat agccttgctg ccgttaaatc tggcaccaaa gctaaacgtg
     7081 ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg cgaaactaaa acctggactg
     7141 gccaaggccg tactccagct gtaatcaaaa aagcaatgga tgagcaaggt aaatccctcg
     7201 acgatttcct gatcaagcaa taatcttttg tagattgcac ttgcttaaaa tcccgccagc
     7261 ggcgggattt tttattgtcc ggtttaagac aatttaataa gaataagcta taaaaaaacg
     7321 gcgtcgattg ctcaacgccg tttcgtggat aacaccgata cggatgttac ttcttaatgc
     7381 ccatctcttc ttcaagccag gctttaaatt ccgtgccaag ggtgttatga cgaataccgt
     7441 attcaacgaa ggcctgcatg taacctaatt tattaccgca gtcatggctc ttccctttca
     7501 tatgataggc ttccaccgtt tctttttcga tcagcatatc aattgcgtcg gtgagctgaa
     7561 tttcatcacc agctcccgga ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta
     7621 cgtaacgacc cacaatagcg agattagacg gcgcaacatc cgctttcggt ttttctacca
     7681 caccaaccat cggtacgctt tcacccggcg ctaattcaac gcctttgcaa tccacaacgc
     7741 catatgcggt cacatcagca accggttcaa ccatgatctg gctatgaccc gtttcatcaa
     7801 agcggcggat catctctgcc aggttatcct gtgacaaatc ggattcatat tcatccagaa
     7861 taacatcagg caaaataaca gctaccggtt catcacccac taccgggtga gcacacaata
     7921 ccgcgtgtcc caggcctttc gccagaccct gacgaacttg cataatagtt acgtgcggcg
     7981 gacaaataga ctgcacttca tcaagcagtt gacgttttac acgtttttcc agcattgctt
     8041 ccagttcaaa actggtatca aagtggtttt caatagagtt tttagatgag tgtgtaacca
     8101 gcacaatttc agtaatgcca gccgcaatac attcattcac gacgtattga attaatggct
     8161 tatcgacaag tggcagcatc tctttcggga tggctttcgt cgccggcaac atcctggttc
     8221 ctaatcccgc aacggggata acggcttttt tgactttcgt attaatggca gccatttaaa
     8281 ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat cccagtatat
     8341 cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt tagcataatt aagacaatta
     8401 cttatacgaa tggcgacgat agtaccacca gccgacatta gcaggtaatg caaatttagc
     8461 ccgcgttatc gtttgctcat tctgcagaca acatcaagcg cagtcgacca ccggttcccc
     8521 atatttggca ttgccaggca tcgcatcgct ggctcaattg attcaaataa gcgttaccta
     8581 aagtgcctaa cggaacacca ttactgattt gcacctggtg ttcgccggta tttaacgtcg
     8641 cattcagacc cgcagaaacc agaatgagat ttttcagttc gcgatgataa tagccaacta
     8701 atagcggaaa ctgccccggc agattggcct gacggagtaa atggtttacc tgcttcaata
     8761 acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt
     8821 taaacaatgc gcgtaataac aaggcagcaa gtacgccatt atgtccagct cgggtgacat
     8881 caaggcaata aaatgccaga tcgttttccg aaagtgcggc aatatcaagc accaggccgg
     8941 gtttgtccgc ggcaaccaat tgacgataat taacccggca atgggaaatc acctgctgaa
     9001 ccggcggttg tagttcctgt aataatttcg ccgctgcggc agggttatca accattgcat
     9061 cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga attaaacatg ctgggataga
     9121 gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt aactggtttc agcaaaacat
     9181 cttcaacgcc cagacgtaac gctttggcaa tatctgccat attttcagtg gcagatatca
     9241 ccagaactgg ggtctggtcg cctctgttac gtatatgctc cagcagttta agcccgttca
     9301 ttcgtggcat cgcgatatca catatcatca ggtctggagt gaaacctccc agcaactcaa
     9361 gggcatccac cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa aaccatgaat
     9421 ccagaagcga gcgaaatacc tgctcatctt caacaatgag aatctgtttt ccgaccaatg
     9481 gctgcgtcat gttctctccc ctgactggct ttactcaata gtggcatgct attgccacct
     9541 gcgcctgtca gaattaactt aagtgtagat aaaaattctg gtcaaatgtt ggtgcgtacc
     9601 aacggcaaaa gttcgtccat tttcttttcc actgccagct gtccggccgc aatggcagcg
     9661 tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat cagaatatcg
     9721 ggcggatcac ctgccatgcg gttcctttta aggcggttct ccagcacctg gatagaagtg
     9781 gtcataatct ctgttgccgt tggtgctgtc accgcccgac gtgtcgttat gctgcctaac
     9841 ctttctttca gacgcgcatg ccacggcaga gaatcaccat tctcgctatt ttcttcactg
     9901 acattaaagg agagcaaatc ttgttgcatc aaatgagcat cgtgctgcag gtcaaccgct
     9961 atcacaatat cagcccccaa tgcacgcgtg agggaaatag gaattgggtt aacgactgct
    10021 ccatcaacca gccagtagcc gttatgtgca acaggtgcca tgagtcctgg aatactgcat
    10081 gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc ataattcacg tcccgtactt
    10141 aaattggtgg caacagccgc aaagcgacgg gaacaatttt cgatctctgt ttccggcatt
    10201 atttcgcggt attgattgaa gacacgctcg ccgcgcagta acccaccgcg ctgccaggag
    10261 agatccatca ggcgtaaaac atcccaataa ctgaaagagg tcacccaatc ttccagcgca
    10321 gataatcgat cgcatgcata ggcagcgccc accagcgaac caattgaaca tcctgcaacg
    10381 atatcaattt caatacccac tttttttagc gcattaataa cgccaatatg cgaccaacct
    10441 ctcgccgcgc cagatcccag cgccagccct atttttatct ttctcattat tcccgcaaga
    10501 ttcccctgga atgcaatcgt agccacatcg ctactgctcg gttaacatag tgctactcag
    10561 gcggttaata cgccgttatt gtttccaggg agattatttg tgtctcagct ttgtccctgt
    10621 ggtagtgctg tcgagtatag cctatgttgc cacccttatg tgtctggtga aaaggttgca
    10681 cctgatccag aacatctcat gcgttcgcgt tactgcgctt ttgtgatgca agacgcagat
    10741 tatttaataa agacctggca tccctcttgt ggagcagcgg cgttacgtgc cgaattgatg
    10801 gccggatttg cgcatactga atggttggga ttgaccgtat ttgaacattg ttggcaggac
    10861 gccgacaata tcggatttgt cagttttgtt gctcgattta ccgaaggtgg taaaacaggt
    10921 gcaataattg aacgttcgcg atttttgaaa gaaaacggtc aatggtacta tattgacggt
    10981 acacgtccgc agtttggtcg caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa
    11041 aagtgctgcg gccaataatg gttgacggta cggtttagca aacactctca acaaggtttt
    11101 tccagcaatg cattcactcc aacgtaaagt tctgcgtact atttgtccgg accaaaaagg
    11161 tctgatcgca cgtattacca atatttgcta caagcacgag ttaaatatcg tacagaacaa
    11221 tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc acggaactgg aagggatttt
    11281 taatgattcc accctgctgg cggatctcga tagcgcattg ccagaaggct ccgtgcgtga
    11341 gctgaatcct gccggtcgtc gccggatagt gattctggtc actaaagaag cgcattgcct
    11401 tggcgatttg ttgatgaaag ccaattacgg cggcctggat gtcgaaatcg cggcagttat
    11461 tggtaaccac gatactttac gttctctggt tgagcgtttt gatattccgt ttgagctggt
    11521 aagccatgaa gggttaagcc gcaacgagca cgatcaaaag atggcggacg ccattgatgc
    11581 ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg gtattaacgc cggaatttgt
    11641 ggcacgcttc ccgaataaaa tcatcaatat tcaccattca ttcctgcctg cgtttatcgg
    11701 cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag attattggcg caaccgctca
    11761 ctatgtgaat gacaatctgg acgaaggccc aatcatcatg caggacgtta ttcatgtcga
    11821 tcatacctac acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt
    11881 aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt gtttacggta atcggacgat
    11941 tattctttaa tcgccagcaa aaataactgg ttacctttaa tccgttacgg atgaaaatta
    12001 cgcaaccagt tcatttttct caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg
    12061 cgccccgctt cccgataagg gagcaggcca gtaaaaagca ttaccccgtg gtggggttcc
    12121 cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc
    12181 ttcccccacc accatctcaa gtattacctc aaaatttgaa ttacccctgg tggggttccc
    12241 gagcggccaa agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct
    12301 tcccccacca ccatcacttt caaaagtccg tgaactctca aacgaatccg cgattaaata
    12361 ttctgcccat gcggggaagg atgagaagcc tcgaccaagg ttcgactcga gcgccagcga
    12421 gagagcgttg ccgcaggcaa cgacccgaag ggcgaagcgc gcagcgttga gtaatccttc
    12481 ccccaccacc atcacttcct taaataatca aaacctgtta ctccgagcta attttgcccg
    12541 atggcaccac gtttaccggg cctacaatat cctcccctgt aggtcggata agcgaagcgc
    12601 atccgacaac tcaccccgca ccgtgcccat aaaaaaaccc cgccgaagca gggtctatgt
    12661 gaactaaaat tcgcttagtg acgagcgcgc accagctggt acttacgtgt cagatactcc
    12721 accggtacgc tccagatgtg taccagacgc gagaacggga acagcaggaa taacgtcatc
    12781 cccagcacca ggtgcagacg gaagataaac gccacaccat cgaggtgttg agaagcgcca
    12841 ccgtggaagg tcaccaccga ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc
    12901 atatgctgag cggagaacgg aatggtcaac aggcccagcg cgcactggat aacgagcagc
    12961 gacaggatca ggatatccgc tccggtagtg gttgcacgca cgcgtgggct gaacagacga
    13021 cgcttcagca acagcacgcc gccaatcaga cacagcacgc cgctggcacc accagcaaac
    13081 atcgccattt tctgtttcac ttcaatcggc agccaggctt catacatcca gtgcggcgtc
    13141 agcataccga agaagtgacc aacaaaaatc cccagaatac cgatatggaa caggttcgac
    13201 gccaggttca tccctttgcg atccagcatc tggctggacg ccgcgcgcca ggtgtactgc
    13261 ccgtagtcat aacgcagcca gctaccaatc aggaagaccg ccccggctat gtacgggtag
    13321 atatcaaaga agaacatatt caggaattgc attagtgctg tcctccggtg gtgatattca
    13381 gatattgcgg cgcgacggca ccggcaaagc gacgctgatg agcagtgatt gctgaatcac
    13441 cgcagccttt gtcagcaaag aatttaacct gctcttcttc ccagacagca tccagcgcct
    13501 gcggcgtatc atcgcgcgct tcgtcggcaa ttttttccgc cactttgtcg ctgtcgatag
    13561 cggtattggc caatttcagc agcagatcaa acatcacggc ataacggctt tcacgctgtt
    13621 gcagacgcgc gctcagcaat gccagaatcg gcgcgatatc tttcaaacct tccacggctt
    13681 cgctttgcgg cagctgcgac aggtactcca gatacagcgg cagatggtcc ggtaattcgc
    13741 ggctgtttaa ctgcaagccg tgctgctcgt actgcgccag caggtccacc atcgcctggc
    13801 cgcggtcacg ggattcaccg tgaacatgtt caaatagcaa cagtgacgtg gcgcggccac
    13861 ggtcgaacag ttcgctgtac tgggcctggg catcgagcgg atccatcgcc gttaaatcgc
    13921 gcaggaaaat gcccagcgca tgggcatctt ctttcgacag atttttcgac gcagcaatcg
    13981 cctcaaacat ctcttgttga tgctgccata aggcagcatc cggatattca aggagacgcg
    14041 atacaatcac gagttcgatc atggatgcgg ctccgttttg ctggtcacat cgatggcatc
    14101 gatacgacgg ctgttgaaca gattgaattt ggtatctgaa ccgtggcagc catcaccaaa
    14161 ggtaaagccg cagccatttt tctccgggaa ggcttcccgt gccagttcac gatgactact
    14221 cggcaccaca aagcgatctt cgtagttagc aatcgccaga taacggtaca tctcctgtgc
    14281 ctgggcttcg gtcagaccga cctcttccag cgcacgggta tcaactttac cgtcaacggt
    14341 ttcagcacgt ttgtaatgac gcatcgccag catacgtttc agtgcgcgca gtaccggttt
    14401 ggtatcaccg gcggtcagca gattcgccag atactgtacc ggaatacgca agctttcgac
    14461 gtctggcaga atgccgttgc tacccagctc acccgcgtct gccgcagact gaatcggaga
    14521 cagaggcggc acgtaccaga ccatcggcag tgtgcgatat tctggatgca gcggcagcgc
    14581 cagtttccat tccattgcca ttttataaac cggcgactgc tgtgcggctt caataacgct
    14641 cagcggaata ccgtctttaa tcgcctgctc gatgactttc ggatcgttcg gatcgaggaa
    14701 cacctccagc tgacgctggt aaagatcttt ctcgttctcg gtgctggctg cacgttcaat
    14761 cgcgtcggca tcgtacagca gcacgccaag ataacggata cgaccgacac aggtttctga
    14821 gcacacggtc ggctgacccg cttcaatacg cggatagcag aagatgcact tctcagactt
    14881 accgctcttc cagttgaagt agattttttt gtacgggcat ccggtgatgc acatacgcca
    14941 gccacggcat ttatcctggt cgatcaggac gatgccatct tcttcacgct tgtaaatcgc
    15001 accgctcggg caggtcgcca cacatgccgg gttcaggcag tgttcgcaca ggcgcggcaa
    15061 atacatcata aaggtgtttt cgaactggct atacatcgcc ttctggatgt tgtcgaagtt
    15121 cttgtctttc gccagtttgt caaactcacc acccaggtca tcttcccagt tcggcccttt
    15181 ttcgattttc gccatccgtt ccccggtaat cagcgaacgc ggacgggcaa tcggctgcga
    15241 tttgctgcct tccggcgcgg tatgcaggtt ctgatagtca aaatcgaacg gctcgtaata
    15301 atcgtcgatc cccggcagat gcgggttagc gaagatttta cccagcaaca tggcacggtt
    15361 acccatgcgc ggctgcagtt tgccgttgat tttacggatc cagccgcctt tgtatttttc
    15421 ctggttttcc cagtcagtcg ggaagccctg acccggcttg gtttccacgt tgttgaacca
    15481 cgcgtattcc acgccttcac ggctggtcca gacgttttta caggtaactg aacaggtgtg
    15541 gcagccgatg cacttatcga gattcagcac catgccgact tgtgaacgaa ttttcatttt
    15601 acgctctcct gtacctggtc attgccttcg ccatctaacc agtcaatgtt cttcatctta
    15661 cgcactacaa caaactcatc gcggttagaa cctacggtgc cgtagtagtt aaagccgtat
    15721 gccagatggg catagccgcc gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgag
    15781 ttatggatac caccacgctg ttgggtaatt tccgaaccag gcaggttaac gatacgttcc
    15841 tgcgcgtggt acatcatggt catccctgcc ggaacacgct ggctgacaac agcacgggca
    15901 gtcagagcac cgttgctgtt gaatacttca atccagtcgt tatcggcgat acccagatct
    15961 ttggcatcgg cttcactcaa ccagaccacc ggaccaccgc gacctaaagt cagcatcagc
    16021 aggttgtcgc tgtaggtgga gtggataccc cacttctggt gcggcgtcag gaagttgagc
    16081 gctttttccg ggttgccgtt ggatttctgg cctatcactt ctttcaccga acgggtgtcg
    16141 atcggcggac gataaaccag caggctttca ccgaaatcac gcatccactg gtgatcctga
    16201 tacagttgct gacgaccaga gagcgtacgc catgggatca gctcgtgaac gttggtgtaa
    16261 ccggcgttgt aagaaacgtg ttcatcttcc agaccagacc aggtcgggct ggagataatt
    16321 ttgcgcggct gtgcctgaat atcgcggaag cggatcttct cgtcttcttt attcagcgcc
    16381 agatgcgtat ggtcacgacc ggtaaattcg ctcagggcag cccaggcttt cacggctacc
    16441 tgaccgttgg tttccggtgc cagtgtcagg atcatctctg ccgcatcaat tgcggtgttc
    16501 agcatcggct ggcctttcgc cggaccttcc gctttggtgt agttgagctt acgcagcaga
    16561 tccatctcgc tctgggtgtt ccaggcaatc cctttaccgc cattaccgat tttctccatc
    16621 agcgggccga tagaggtaaa gcgttcgtaa gtcgccggat aatcgcgctc tacgaccata
    16681 atgtgtggcg cggttttacc tgggatcagg tcgcactcgc cttttttcca gtctttcaca
    16741 tccagcggct gcgccagttc agcggcagag tcatgctgga taggcagcgt gacgatgtcg
    16801 gtttctttac ccagatggcc gacgcacact tcggagaatt tcttcgcgat ggctttgtag
    16861 atttcccagt cgcttttcgc ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata
    16921 aacggatgca tatccgaagt attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa
    16981 atgatgtcgg aatagagaca ggtgctcgac agacggaagt ccagcgtcac caccagatcc
    17041 agcttgcctt ccagaccatt gtcctgccag tccacttctt ccggcttcac gccgccctgt
    17101 tgccccagat ctttaccctg gataccgtgc tccgtcccca gcaggtactt gagcataaac
    17161 tcatgacctt taccggaaga accgagcagg ttagaacgcc agatgaacag gttgcgcggg
    17221 tggtttttac cgttttctgg ttgttctgcc gcaaaacgga tggaaccctc tttcagggat
    17281 ttcaccgtat agtccaccgg attcatcccg gctttttctg cttcgccagc gatagtcagc
    17341 gggttagtgc ctaactgcgg cgcagacggc agccagccca tgcgttctgc acggacgtta
    17401 aagtcgatca ggtgtccggt ataacgggat ttatccgcca tcggcgacag cagttcttcc
    17461 gcagtcacgg tttcataacg ccactggctg gagtgattat agaaataaga agtgctgttc
    17521 atatgacgcg ccggacgctg ccagtcaagg gcaaacgcca gcggctgcca gccggtttgc
    17581 ggacgcagtt tttcctgacc tacatagtgc gcccagccgc ccccgctctg accgacacag
    17641 ccgcagaaaa tcagcatgtt gatcagaccg cgatagttca tatcgaggtg ataccagtgg
    17701 ttcagccccg caccgacgat aatcatcgaa cgaccgtgcg ttttatcagc gttatcggca
    17761 aattcacggg cgatgcgaat aatctggctg cgagaaacgc cggtgatctg ctcagcccag
    17821 gccggggtgt aagctttcac atcgtcatag ctggttgcac agttaacgtc gttcaggcca
    17881 cgttccagac cgtagtttgc cagcgtcaga tcataaacgg tggtcaccag ggcggtgctg
    17941 ccatcagcca gttgcaggcg tttcaccggc agtttgtgca gcagcacgtt ttccagttcc
    18001 actttgttga agtgttccgt gccgtcgcca ccaaagtacg ggaagcctac ctcagcgatc
    18061 tcatcctggc tacccagcag gctcagttgc agctcggttt cttcgccagt tttgccgtcg
    18121 cgctgctcaa gattccattt gcccttctcg ccccagcgga agccaataga accgttcggc
    18181 gcaaccattt cgccattggt attaaaggcg acagttttcc attccggatt gttttcctgg
    18241 cccagcgcat caaccagatc agcagcgcgc agcatacgac ctgcagcgta gtaaccgtcg
    18301 cgttcttcca gcatcaccag catcggcatg tcggtgtagc gacgcacata gtcggtgaaa
    18361 tactggcttg ggttgtcgag gtggaattca cgcagcatta cgtggcccat cgccagcgcc
    18421 attgccgcat cggtgccctg tttcggtgcc agccacagat cgcacagttt ggcgatttca
    18481 gcgtagtctg gtgtgacggc aacagttttg gtccctttgt aacgcacttc agtaaagaag
    18541 tgagcatccg gggtacgcgt ctgcggtacg tttgaccccc aggcgatgat gtagctggag
    18601 ttgtaccagt cagcagattc cggtacgtca gtttgctcgc cccaggtttg cggagacgca
    18661 ggaggcaagt cgcagtacca gtcgtagaag cttaagcaag taccgccaat cagcgagaga
    18721 tagcgtgcac ccgatgcgta agaaaccatc gacattgccg gaattggcga gaaaccagca
    18781 acacggtccg ggccgtagtt tttgatggtg taaacgttag atgcggcgat cagttcgttc
    18841 acctcctgcc aggaagaacg aacaaatcca ccgcgtccac gcgcctgctt aaagcttttc
    18901 gctttatcgg cgtcttcaat gatagaagcc catgcctcaa ccggatcgct atgcagcgcc
    18961 ttcgcttcac gccacatttt catcaggcgt ttgcgcatca tcgggtattt caggcggttg
    19021 gcgctgtaaa gataccagga gtagctggca ccgcgcgggc agccgcgagg ttcatggttt
    19081 ggcagatccg gacgggtacg cggatagtca gtctgctggg tttcccaggt gaccagaccg
    19141 tttttgacgt agattttcca gctgcaggag ccggtgcagt ttaccccgtg ggtagagcgg
    19201 acgattttgt catgctgcca acgctggcga tatccatcct cccagtcacg gttggtattg
    19261 agaagctggc catgcccatc ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg
    19321 tccaggaatt tactcatcgg ttttctcctg tgggagcctg tcggccctct gataaatcga
    19381 cattgctaat gtttaagggc aaggtaacgc tctgaaacag cgtgggaatt gataacgatc
    19441 aagtgtgatg gggatgaaaa ataaagtaaa ttcctaagat acccctttag gataattaac
    19501 agttaaagca taacatgctg taaattaaga cgttttatac ctgtagttat ttaaagcatg
    19561 aagattttta tgcagatggg tattaaggag tattccccat gggtaacata ttaatggctc
    19621 ttaatgaaat ccggcgaaat aagccatata acattagtat gttgtaacta aataacgcat
    19681 aaaaaaagcg cgacatcatg ccgcgcaaag gataatcaaa tattttactc tatataattc
    19741 ggtttacagg aaggcggcaa gtgaatgaat cccaggagct tactaaagta agtgactggg
    19801 gtgaatgaac gcagccgcag cacatgtaac ccgaagtatg acgagtaacg gtttattttt
    19861 tagaatgccg accatatacc gcccaggtaa tcactacgca ggcgatatag aaaatcaaaa
    19921 ataccttcat tgcgccgact ggcgaacccg ttaatgccag cgagctacca aacgctttcg
    19981 ggataaagaa gccaccaata gcgccaatcg cagagatgaa acccagcgcc gctgccgtgt
    20041 cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc ttctgctttc acgcgatcca
    20101 ttgtcagttt acggaagatc actgaaatca tctggaaagt ggaaccacta cccagcccag
    20161 ctgtcaggaa cagcgccagg aagaccgcga agaacgccat gaagcttccg ccctgcccgt
    20221 cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc catcagaata aagttcacca
    20281 gcgtgacacg agttccgccc agacggtcag ataatgcacc acctgcagaa cgcgccagcg
    20341 caccaataaa cggcccgaag aaagcgtatt gcagaatctg aacatccggg aactgcgttt
    20401 ttgacagcat cgcaaagccc gcggagaagc cgataaagga gccgaaggtt gccagataca
    20461 gcaggctcat aatccacaga tgaccccgtt tgagtaccgg caactgctcc ttgatggagg
    20521 ctttcgaggt agcaagatcg ttcatgccaa accacgctgc aatggtgaag atggcaagga
    20581 acggcaccca tatccaggac gcattcgcca gatacagctc agtcccatcc ggctgtttga
    20641 cgccctggct accaaatact gcgaaaatcg acagtgatac caccagcgga gcaaccaact
    20701 gcatgacgct gacgcccatg tttcccagac caccattcag acccagcgcg ccaccctgct
    20761 tctgtttcgg aaagaagaag ctgatgtttg ccatactgga tgcgaagttc gcgccagcaa
    20821 agccgcatag cagagagatg atgatgaaga cgctataagg cgtggaggta tcctgcacgg
    20881 caaaacccag ccagacgcaa ggaataatca gaataccggt gctgaacgcc gtccagcgac
    20941 gaccaccgaa gataggaacc ataaaggagt atggaacacg taataacgcg ccagaaaccg
    21001 aaggcagcgc agtcaacata aatagctgat cggtcgtaaa attaaaaccg actttcggta
    21061 agttcaccgc aacagcgctg aacaacatcc atacgcaaaa cgccagcagc agacagggaa
    21121 cggaaatcca caggttacgg ctggcaatac gttgaccgcg ttgttgccag aacgcaggat
    21181 cttccggtcg ccaatctgta atgacagctc cagtagccct ttcgggggcg gatgagtgac
    21241 tcatagacac ctctgatact cgtttcgatg tctgccacct tagtgtctgt agctaaaggc
    21301 aatttgatgt aaatcaaacg ataagataac tttatctatc attgatattt atcattaccc
    21361 atagtgagta cagtgatttc ataaaaatta tgagattttt cacggtgctg taaaatccct
    21421 acccttaccg atgtaaagcg actaaccaca cggcaaataa ggagtaactc tttccgggta
    21481 tgggtatact tcagccaata gccgagaata ctgccattcc agaatgcatc gtcacattca
    21541 ttaaggttat tgctcattta aagcctgaag gaagaggttt acatgcttaa acgttgtctc
    21601 tctccgctca ccctggttaa tcaggttgcg cttattgtgt tgctttctac tgctattgga
    21661 ctggcaggga tggcggtttc tggctggctg gtgcaaggcg ttcagggcag cgcccatgcg
    21721 atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc tgttggcggc agtgccatta
    21781 agcgagaaag acaagccctt aattaaagag atggaacaaa cggcatttag cgccgagttg
    21841 actcgagcag cagaacgaga cggacaactg gcgcaattac agggtttaca agattactgg
    21901 cgtaatgaac tgatccctgc gctgatgcgt gcacaaaacc gagaaacggt gtcagcggat
    21961 gtcagccagt ttgttgccgg gcttgatcag ctggtatctg gttttgaccg caccacggaa
    22021 atgcgcatcg agacagtggt actggtccat cgggtaatgg cggtatttat ggcactttta
    22081 ctggtgttca ctattatctg gttgcgggcg cgactgctac aaccgtggcg gcaactgctg
    22141 gcaatggcga gtgccgtcag ccatcgcgat tttacccaac gcgcaaacat cagcgggcgc
    22201 aacgaaatgg cgatgcttgg aactgcgttg aacaatatgt ctgcagaact ggccgaaagt
    22261 tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg ggctggagca taaaaatcag
    22321 atcctctcat ttttatggca ggctaaccgc cgtttgcatt cccgcgcccc gctgtgtgaa
    22381 cgcctgtcac cggtactcaa cggcttacag aatttaaccc tgctacgtga tatcgaactg
    22441 cgggtttatg atactgatga tgaagagaat catcaggagt ttacctgcca gccagatatg
    22501 acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg tattacccgt tggcgatcgc
    22561 ggcacaaccc tgaagtggcg gctggctgac tctcatacgc agtacggtat tttgctggcg
    22621 accctgccgc aggggcgtca tcttagccat gatcaacaac aactggtgga taccctggtt
    22681 gaacaactca ccgccacgct ggcgttggat cgccatcagg aacgtcagca acagttgatc
    22741 gtgatggaag agcgtgccac cattgcgcgc gaactgcatg attctattgc ccaatctctc
    22801 tcttgtatga agatgcaggt gagttgttta cagatgcagg gcgatgcgct gccagaaagc
    22861 agccgcgaac tgttaagtca gatccgtaac gaactgaatg catcctgggc gcagttgcgt
    22921 gaattgctca ccacattccg cttgcagctc accgagcctg gattacgtcc ggcgctggag
    22981 gcgagttgcg aagagtacag cgccaaattt ggcttcccgg tgaagctgga ttatcaattg
    23041 ccgcctcgtc tggtgccttc gcatcaggca atccacttgt tgcaaattgc ccgtgaggca
    23101 ttaagtaacg ccctcaaaca ttcgcaagcg agtgaggtcg tggtgacggt ggcgcaaaac
    23161 gataatcagg tcaaactgac cgtacaggat aacggctgcg gcgtgcctga aaatgccatc
    23221 cgcagcaatc actacggcat gataataatg cgcgaccgtg cgcaaagttt acgaggcgat
    23281 tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg tggtcacctt tattcccgaa
    23341 aaaactttca cagacgtcca aggagatacc catgagtaat caggaaccgg ctactatcct
    23401 gctgattgac gatcacccga tgctgcgaac tggcgtaaaa cagcttatca gtatggcacc
    23461 agatatcacc gtggttggcg aagcgagtaa tggcgaacag ggtattgaac tggcggagtc
    23521 tcttgatccc gatctgatcc tgttagatct caatatgccc ggcatgaacg gtctggaaac
    23581 gctggataaa ctgcgcgaaa agtccctctc agggcgcatt gtggtattca gcgtctctaa
    23641 ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg gatggctatc tgttaaaaga
    23701 tatggaaccg gaagatctgc tgaaagcatt gcatcaggca gctgctggcg aaatggtatt
    23761 aagcgaagca ttaacgcctg ttctggccgc cagcttgcgt gctaaccgtg ccactactga
    23821 gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt ctcaagctga ttgcccaggg
    23881 tttgccgaac aagatgattg cccgccgcct ggacatcacc gaaagcacag taaaagtgca
    23941 cgtcaagcac atgctgaaga aaatgaagct caagtctcgc gtggaagcag cggtatgggt
    24001 gcatcaggag cgcattttct gattacggtt cccagcgcag ttcgtcgttt gacaatgcat
    24061 cgaacggttc gacaagcgtt agcgtgatct cattggagga gacacgctgc ccctggttat
    24121 cttccaccac cacagacaat cgccagtgat tgctcgccct ttccccgttc tgccagtcag
    24181 gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc gcccggcgtc aaacttaata
    24241 tctgcgtatc gccctgccaa atcagttgcc gaataccgta acgactgcgg atttgtaatt
    24301 tcagcggcac tgtttcgcca ggttttagat cccacggcgg tgtcgccaga aacaccgtta
    24361 acgtttttcg ctgtcggtac tcaagagtcg gtagattatt tcgctgcgga ttgtcatagc
    24421 gactaccacg taacgactga ctttcggcaa cctcacccgc agaaagttgt tttttgagcg
    24481 gtacaccaaa gcggtaatta agattcagcc cgaggttatt ttgattctcg ccactttcac
    24541 cctgtttatg ctgggccgtc acagtgacta atggcacagg ggtgtaattt aatcccagac
    24601 tcaacgcgac gggattgtga taacccgtac cagagttaaa caaatcaaca cgatcaccaa
    24661 aatactgttc tacgctgaca ctggtattga ggtgttgata gaacggcatg cgcatccggg
    24721 ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt ggctgtctgt tcatgccatg
    24781 cagcaaacgg ctgataaaag tttgccgata gtcgcaaata ttcgccccac gcttcggcac
    24841 caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc ataaaaagtg ttataaccca
    24901 ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac attgctcacc aacccatcat
    24961 cctgctgagt aagaccaagc tggctccagg tgagataacg atcattatct tgtaacggca
    25021 caaaccagct tccacgactg ccggtgaaat gtccttcgtt atccactttg acgtcaacac
    25081 tggcatttcc ccacggtgat agccaggact ctacgtgctg attaacctgt tgactaagcg
    25141 cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc gccagtatcc agcccgttat
    25201 cattcatact ggtttcgccg aaatctttca caatttcagc aaagtgtttt tccccatcat
    25261 gattttcggg agccatgcct aaatccggca gaccatcatt gttattatca aagggatttg
    25321 ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc gcctgctaca agcaagagta
    25381 aataaaacgg gataatgcga ggaacgaaac ggctcaaaac gtcggtaact tccggtaata
    25441 atgaaaaacc atagccttaa agaataacga ttactggcaa cttttgtcgt attttctcgc
    25501 gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga agcagtaaga
    25561 tagaagatga gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa atcgtgatcg
    25621 ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg ctggccattg
    25681 cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctcatgtct gatgcggtca
    25741 cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa atgctggaga
    25801 tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac gggcgcggga
    25861 ttagtacact tcctctgatt gatggggtgg aaatcggtac tcttgtggaa ctggcgcaat
    25921 ggacgctgtc agccgataaa gtgctcacat tttaatgttt gagggggcgt ctgacgcccc
    25981 actatgcggg aagtttactc aggcgaatcc cggacgtaac acaccatccg gaaaattctc
    26041 cgccagcagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct gcattcccag
    26101 tttgataagc tcctgttcca gtgaaaacag gtattgtgcg ttggttccca gcggaccgct
    26161 cgccgccgca atcaacggcg cgatgacctg agcgcgagta tcagattcat attctggatg
    26221 tcgcggatcc ataataaaca caatggcgtt tactgtgcgt ccatcatcaa gatcaagctg
    26281 acaccaggtt ggcagataac agccggtaat catctctcgc ttccacaaca aggttagttc
    26341 ctgctccagc gtctcttctg gcagtcgata ggcgacgcct gtggtgcgtc cgccctcttt
    26401 cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca ggcgcaggca
    26461 aaatgcgcga tgccatccaa ccagtgtacc ggtgcacgat tcggtaaact ccagtgccgg
    26521 attccacatc aacgatccat agccgaagat ccacaccggt tcctcatcag gtcgacaagc
    26581 cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac cataagagtg attcttcaat
    26641 ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta tcaccttaca
    26701 cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac atttttactg
    26761 ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc aaggcaacac
    26821 cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat ttatcatcat
    26881 cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgcg gtttcttcgc
    26941 gactggcgtc atcacgccga tcgtctttat ctttatattg atcccatgcg ctgttgaacg
    27001 cttctttata gatatcctgg gcatgagacg gtagaacgtg ctttacgctt tccggcagat
    27061 cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat taagtgtggt
    27121 caataatatc tactcttgcc aaaacataag aattttcatg tcagactctt cttaagaaac
    27181 gctaaaacaa acattgtcta aacgtaatac ttatttttat gccccaatga gtaaaaaatc
    27241 gacagcgggg taaaaattcg taaaatgtta ccgatatcca gacactttct gttattttcc
    27301 ctacctattt tttacatttt cgataaattc atctgcaaag ggataaccat aatgtcaaat
    27361 gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc ggttctggcg
    27421 ctggtagtgc tgttcctgtg gggaagtagc cagacactac cagtggtcat tgccatcaat
    27481 cttcttgcgc ttattggtat tttaagtagc gcctttagtg ttgtccgtca tgcggacgta
    27541 ttagcccatc gcctgggaga accttacggt tcgcttattc ttagcctttc agtggttatt
    27601 cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc aacgctaatg
    27661 cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg cttttcatta
    27721 ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat caagcagtat
    27781 ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc tctgcctgcg
    27841 gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc aatgtatggc
    27901 gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga gcacgaagat
    27961 gacagtgatg acgatgaccc gcatcacggt aaaccgtctg cccatagcag cgtgtggcat
    28021 gctatctggt tgattatcca tctgattgcc gttattgcag tcaccaaaat gaatgccagc
    28081 ccgctggaga cattgctcga cagcatgaat gcccctgtcg cctttactgg cttcctggtg
    28141 gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt gaacaaccag
    28201 gtccagcgcg cgatgaatct gttctttggt tcagtgttag caaccatttc gcttaccgta
    28261 cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tacagtttgc acttggtgcg
    28321 ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc atatctcctt ctccaccgga
    28381 cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta tttgatgacg
    28441 atatttgcct gacagttata tctcctccga tgacggaaat cgggggagat aagataaatt
    28501 cctgcttatt gtatttcttc atactacctg gacagctcgt taatcagaat tgcgagcctg
    28561 taccacaacc tgcacgaaac cactgcaaca gctgttgaaa agccggaatg tactctttat
    28621 tgcaaacatt tcagggatga agtgaagatg aattgagtcg tagtatccac accaagccgg
    28681 aattatccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
    28741 aagagaaaac ccccgcacgt tgagaggttt aacctgacaa caaaacgggg gctaatcttg
    28801 aaaccagaca cttcaagaat agccgcaaaa tgttgtcatt acaacaaggc ggctatatga
    28861 cgctcgcgca gtttgccatg actttctggc acgacctggc agcaccgatc ctggcgggga
    28921 ttattaccgc agcgattgtc agctggtggc gtaaccggaa gtaaggtgaa aacgggcgtc
    28981 aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag cctgcggcca
    29041 tttttgtctt tatctcttcc acaagtaaat attggcaacc cgagctggaa gcatcatcca
    29101 gagtactcat gaaacgactg taacagctgt tgaaaaaccg gaatgcactc ttcattgcaa
    29161 acatttcagg gatgaagtga agatgaattg agtcgtagta tccacaccaa gccggaatta
    29221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    29281 aaaacccccg cacgttgaga ggtaaaacct gacaacagaa cgggggctaa tcttgaacca
    29341 tcaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
    29401 cgcgaagttt gccatgattt tctggcacga cctggcagca ccgatcctgg cggggattat
    29461 taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc
    29521 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccatttt
    29581 tgtctttatc tcttccacaa gtaaatattg gcaacccgag ctggaagcat catccagagc
    29641 cctcatgaaa cgactgtaac agctgttgaa aaaccggaat gcactcttca ttgcaaacat
    29701 ttcagggatg aagtgaagat gaattgagac gtagtatcca catcaagccg gcgttatccg
    29761 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa
    29821 cccccgcacg ttgagaggtt taacctgaca acagaacggg ggctaatctt gaaccagaac
    29881 aattcaagaa tagccgcaaa atgttgtcat tacaacaagg cggctatatg acgctcgcgc
    29941 agtttgccat gattttctgg cacgacctgg cagcaccgat cctggcggga attattaccg
    30001 cagcgattgt cggctggtgg cgtaaccgga agtaaggtga aaacgggcgt caggctgccg
    30061 tgatggcaat atgcgcccga aaccggccgc aggggtaaca gcctgcggcc ttttttgtat
    30121 ctggcaccca tgaaaaaagt cttaacgcag aacgctaaga ctttattttt caagcaaaaa
    30181 agattacttg ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttcat
    30241 ctgcttgagg aacggttcca gtttagccag cggcagcgcg gatggaccat cacatttcgc
    30301 atgttccgga tccggatgcg cttcaataaa cagccccgcc agacctaccg ccataccggc
    30361 tcgtgccagc tcagccacct gagcacgacg accaccggaa gctgcgccaa acggatcgcg
    30421 gcattgcagt gcgtgggtca cgtcgaaaat caccggcgag ttaccagaca ctttcttcat
    30481 aatgctgaag cccagcatat cgacaaccag gttgtcatag ccgaagttag caccacgatc
    30541 gcaaagaatc actttttcgt tgccgccttc tttgaattta tcaacgatat tacccatctg
    30601 tcccgggctg acaaactgtg gtttcttgac gttaattacc gcaccggttt tcgccatggc
    30661 ttcaaccagg tcagtctggc gagcaagaaa cgccggcaac tgaatcacat ccacgacatc
    30721 agcaacgggc tgtgcctgac ttggttcgtg aacgtcggtg ataattttca cgccaaaagt
    30781 ctgcttcaac tcctggaaga ttttcatccc ttcttccagg cccggtccac gataagagtg
    30841 gatggaggag cggttggctt tgtcaaaaga ggctttgaac acgtaaggga tacccagttt
    30901 ctgggtcaca gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag attccagcac
    30961 gttcataccg ccaaacagta cgaacggcag gtcatttgct acgttgatgt cgccaatgct
    31021 aaccactttt tgtttcatag gatcgcctta atcttgagta aaatgtcgat taattaatgc
    31081 agcacaatat gtttatgcgc gatgttattt atttgcgcac ggatcatttc gctgatcggg
    31141 tcttccggac actgttcaac gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc
    31201 agttgcgcat aaatcaaccc gcgatcgcga atttcatagg gatcttcagg gttgaattgt
    31261 aataaagctt cgctggtgcg taacgccagc tccatctgat tttcttccat caacgaggct
    31321 ttgagtgtat ccagcaattt gcggattacc tcaatgttat cagcttcatc aaggtcttca
    31381 taaaacagtt ccgccgacgg gctgatattt ccctttaacc atacgtccag catatgttcg
    31441 cttaacgatt caccgttaaa aggattaatc agccaaattt cgccatccgg acattcaatg
    31501 cgcaatatca gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg
    31561 acccataata aaaccgcacc taatgatacc gcactgccct gtcgattctt taacacctgg
    31621 tccagccata atgcatcgga aagacgataa acgccgcgtg aggctttaaa tccccagtcg
    31681 ccgtaaaaca gcgcgatcag tttttccaac tgctcttcta aaggcagaag ctggctgatt
    31741 tcttccttcg ccagactaac gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa
    31801 tcgcggcgga ttgcttcgca agccaggatc atgccttcgc acaatggcgc tttattaaat
    31861 tcgaaatcag ctaacgatct catgacttac cccagtaacg gtacttttgt ggtggcgagt
    31921 ttaatgatga tgtacagcac caccaacgcc agcgggaagg caataatgcg cgcctgctga
    31981 ctgtgcatac gacgataatc gagtgcaata aaacccaaaa cgatataaat gataactcca
    32041 aacagctttt cagtcagcca ctgtgcctgc ccggaaaatg gcaggatgtg cgctttagcc
    32101 atcaacgcaa taccgcttaa cagtaacagc gtatcgacaa ccggcggcac aatgcgcgtc
    32161 cagcgagcaa atgcctgtgg atgcttctga taacgtagcc agaaacgtaa ggttaatagc
    32221 ccaacagaaa gcgcgagact aataagatga acactaagca gtgtagaaaa acttgtcatt
    32281 gataatagcg gccgagcgtt acgcgctcgt tatcaccata gtcacggcag gtttcgacgt
    32341 catgataccc cgcgaggata aatgcttgtc gcaccgcttc gccctgctgc cagccatgtt
    32401 ccagaagcag aaagccgccg gataccagcg cgttacgcga ctgttcgatg atatgcacga
    32461 tgtctgccat tccactgtct gccgcaacca gcgcagtgag cggctcaaag cggacatcgc
    32521 cttgttgaag atgtgggtcc tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa
    32581 actgctgccc ggctagcgcg ctaaaccagt cgctttgcag aatgtggata tttttgatcg
    32641 ccagatgctg ggcattacgt tgtgccaggg agacagaatc aggcatacga tctacagcga
    32701 taatttcgca gtccgggcgc tcgctagcca gcgcaagcgc aatcgccccg gtacccgtcc
    32761 cgagatcaag aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac
    32821 actccgtatc cgggcgcgga attaaggtcg ctggcgaaac aaataacggc aacgaccaga
    32881 attctcgcac cccggttaaa tgagcaatgg gttcaccatc gcgacgacgt gtcagtagcg
    32941 catcaagttg ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg agaataaaag
    33001 tacgccctct gccggtaaca tgctccagca ggatttcagc atcacgccgc gggctttcgc
    33061 tcgcctgaag ttggcttatt gcttcacgta accagtgttg atattccatt attcctgctc
    33121 ggacaacgcc gccagttggt cggcctgatg ttcctggata atcggttcaa tcagcatatc
    33181 cagcttacct tccatcactt catccaggcg gtagagcgtc aggttgatgc ggtgatcggt
    33241 aacgcgcccc tgcgggaaat tgtaagtacg gttacggtcg ctgcgatcgc cactccccag
    33301 caggttacga cgggtagacg cttcggcctg ttggcgtttt gccatttcag cagcgtggat
    33361 gcgagcaccg agcactgaaa gcgccttcgc tttgttttta tgttgtgaac gttcatcctg
    33421 acattcaaca acaatcccgg tcggcaagtg agtaatacga attgccgaat cggtggtgtt
    33481 aacgtgctga ccacccgccc ctgacgagcg gaaagtatca atgcgtaaat ctgctgggtt
    33541 gatgtccggc agttctgcgt caggcagttc tggcattacc gcaacggtac aagcagaagt
    33601 atgaatacga ccctgcgatt ccgtagcagg aacacgctgc acgcgatgac cgccagattc
    33661 aaatttcaga cgaccataca caccatcacc gctaattttg gcgatgatct ctttataacc
    33721 accatgttca ccctcgctgg cgctcatgat ttctacccgc cagcggcggg cttcggcgta
    33781 acggctgtac atacggaaca gatcgcctgc gaacagcgcc gcttcgtcgc cgccggttcc
    33841 ggctcggact tcgaggaagg cgttacgttc gtcatcagga tcttttggca gtaacagaac
    33901 ctgtaattgc tgttccagtt gctcgctttt ttctttagct tcgcgcagtt catcctgcgc
    33961 catctcacgc atttcaggat catcgagcat catctgtgcg gtttcgatat cttcctgaac
    34021 ctgttgccag tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa
    34081 tgcgcgaaaa cgttcctggt cggcgatagt ttgcgcgtca cccagcaacg cctgaacttc
    34141 ttcatggcgt tcatgcaggg cttccagttt ggcaacgata gaaggcttca taggcgtaaa
    34201 tgcaccctgt aaaaaaagaa aatgatgtac tgctactcca gcccgaggct gtcgcgcaga
    34261 atattcaggc gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt cgttggcgca
    34321 tggatcaagc ggttagtcag tttccatgcc agatcctgca taatggcttg cgcgtcgccg
    34381 ccctgctcaa gggccgctaa cgctttggcg gttaactcat cgcgaacgtg ctctgcctgg
    34441 ctgcgatact cgcgaatggt ttcgctggcg ctttgtgctc gcagccacgc cataaattcg
    34501 ctggtttcct gagcgacaat agtttccgcc tcaaccgctg cggctttacg ctgcgccagg
    34561 ttgtgcgaaa tgatgctttg cagatcgtcc acactataaa gataagcatt cgccagtttg
    34621 ccaacttccg gctcaacatc gcgcggaacg gcaatatcca ccaacagcat tggttggttg
    34681 cgacggcttt ttaatgcgcg ctccaccatg cctttcccga taatcggtaa cgggctggcg
    34741 gtggaactga tgatgatatc ggcttcgcgc agacgttcgt cgatctcact cagggcaatc
    34801 acttccgcgc caacttcatc tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata
    34861 atcatcttct gtactttatg ttcgcgcaga tgacgcgcta ccagctcgat ggtttcgccc
    34921 gcgcctacca gcaacactgt gaccgtagag agcgattcaa agatctgccg cgccagcgta
    34981 caagccgcaa aagcgacaga cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt
    35041 ttcgctacag agaaagattt ctggaacatg cgttccagtt cgctggcctt catatgacct
    35101 ttttgcgaat cggcaaacgc ttttttaacc tgaccgagga tctgcggctc cccaagaacc
    35161 agtgaatcca ggccgctggc aacacgcatt aaatggctaa ccgcgtcgtt atcctgatgc
    35221 cagtagaggc ttttacgcag atcttcttca ttaagattgt gataatcgca aagccagcgg
    35281 attaacgcct cttgcaggtt atcctgctct tcaacgctaa gataaagttc cgtgcggttg
    35341 cacgtcgaca gcaccacgcc gccctgcacc atcggctgcg caagcaggct gtcaagcgcc
    35401 tgatcgagct tatccggcga aaacgataca cgttctcgca gcgatacagg tgccgtttta
    35461 tggttgatac cgagtgctaa aagggtcatg tctgcgggaa ataataccaa cgttgatagg
    35521 gttagtctgc ttgcatcata caggatgcgt aagatcaata aaagagagcg cccccttttg
    35581 gagtaattgc cgaaagccgt tagattctgg caattaagac aacttgaaca tagacgatag
    35641 cggacggtaa cgctagcatt aagggttata actgcaacgt atctcaagga cttgtcatca
    35701 ctatgcccct gcccgatttt cgtcttatcc gcctgctacc gctggctgct cttgtgctca
    35761 ctgcctgttc cgttaccacg cccaaaggtc ctggcaaaag cccggattcg ccacaatggc
    35821 gtcagcatca gcaagacgtg cgcaatctta atcagtatca gactcgcggc gcgttcgctt
    35881 atatttctga ccaacaaaaa gtgtacgccc gctttttctg gcagcaaacc ggccaggatc
    35941 gctaccgtct gctgctcact aacccattgg gcagcacgga actggagctg aatgctcaac
    36001 cgggtaacgt gcagttagtc gacaataaag gtcagcgtta taccgccgat gacgccgaag
    36061 agatgattgg caaattgacc ggaatgccaa ttccgctcaa cagcttgcgc cagtggattt
    36121 taggtttacc gggtgatgca accgactaca aactggacga ccagtaccgc ctgagcgaaa
    36181 ttacctacag ccagaatggc aaaaactgga aggttgttta tggtggttat gacaccaaaa
    36241 cgcaacctgc gatgccagcc aatatggaac tcaccgacgg tggtcaacgc atcaagttaa
    36301 aaatggataa ctggatagtg aaataatgcg gacacagtgg ccctctccgg caaaacttaa
    36361 tctgttttta tacattaccg gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt
    36421 tcagtttctt gattacggcg acaccatcag cattgagctt cgtgacgatg gggatattcg
    36481 tctgttaacg cccgttgaag gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg
    36541 attgttgata aaaactgcgg cagacagcgg gcgtcttccg acgggaagcg gtgcgaatat
    36601 cagcattgac aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc
    36661 gacggtcctg gtggcattaa atcatctctg gcaatgcggg ctaagcatgg atgagctggc
    36721 ggaaatgggg ctgacgctgg gcgcagatgt tcctgtcttt gttcgggggc atgccgcgtt
    36781 tgccgaaggc gttggtgaaa tactaacgcc ggtggatccg ccagagaagt ggtatctggt
    36841 ggcgcaccct ggtgtaagta ttccgactcc ggtgattttt aaagatcctg aactcccgcg
    36901 caatacgcca aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca atgattgcga
    36961 ggttatcgca agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata
    37021 cgccccgtcg cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg atacagagtc
    37081 tgaagcccgc caggtgctag agcaagcccc ggaatggctc aatggctttg tggcgaaagg
    37141 cgttaatctt tccccattgc acagagccat gctttaagcc gggcaagctg agtttcggtg
    37201 acaacgtcac cttgttccag acgttgcatc gcgctcttta atacaccgcc tggaaaggat
    37261 catgcctggc ccgcacagtt ttcggcagat tctttccacc aatggacgca tgcctgaggt
    37321 tcttctcgtg cctgatatga agctttttgc tggtaacgcc accccggaac tagcacaacg
    37381 tattgccaac cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg
    37441 cgaagtcagt gtacaaatca acgaaaatgt acgcggtggt gatattttca tcatccagtc
    37501 cacttgtgcc cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg
    37561 tcgtgcttcc gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga
    37621 ccgtcgcgtc cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc
    37681 cagcgtcggt gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt
    37741 cttcgacgtt ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca
    37801 gctgaatctg gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg
    37861 cgctatcgct aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg
    37921 tgcgaacgtt tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact
    37981 ggtcgatgat atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag ctctgaaaga
    38041 acgtggtgct aaacgtgtat ttgcgtacgc gactcacccg atcttctctg gcaacgcggc
    38101 gaataacctg cgtaactctg taattgatga agtcgttgtc tgcgatacca ttccgctgag
    38161 cgatgaaatc aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta tgctggccga
    38221 agcgattcgt cgtatcagca acgaagaatc gatctctgcc atgttcgaac actaatcgaa
    38281 cccggctcaa agacccgctg cggcgggttt ttttgtctgt aatatccatt tgtatgacct
    38341 atgcctcctt cacctgccat ttagttgaca gatgatgcgc tcacggatga aacattattg
    38401 tgaacaaaat attttcctca catgtgatgc ctttccgcgc tctgatcgac gcttgctgga
    38461 aagaaaaata tactgccgca cggtttaccc gtgacctgat tgccgggata accgtcggga
    38521 ttattgctat cccgctggcg atggcgttgg ctattggtag tggtgtggca ccccagtacg
    38581 gtttatatac tgcagctgtt gcggggattg tcattgctct gacgggtgga tcacgcttta
    38641 gcgtttccgg tccgactgcg gcatttgtgg taattctcta tcccgtgtcg caacagtttg
    38701 gactggcagg actgctggtt gcgaccttgc tgtcggggat ctttttgatt ctgatgggtc
    38761 tggcacgctt tggtcgcctg attgagtata ttccggtttc cgtcacctta ggtttcacat
    38821 cgggtatcgg gatcaccatc ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg
    38881 cccatgtccc ggaacattat ctacaaaaag tcggcgcatt atttatggcg ctgccgacca
    38941 ttaacgtggg cgatgccgca attggcattg tgacgctagg tattcttgtt ttctggccgc
    39001 gtctgggcat tcgtttaccc ggtcaccttc cggccttgct ggctggttgc gcggtgatgg
    39061 ggattgttaa cctgctcggc ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc
    39121 tggccgatgg ttctcagggt aacggtattc cgcaactgct gccgcaactg gtgctgccgt
    39181 gggatctgcc taattcagaa ttcacgctaa cctgggattc tattcgcaca ctgctgcctg
    39241 cggcattctc aatggcaatg ctcggcgcaa tcgaatctct gctctgcgcc gtggtgctgg
    39301 atggtatgac cgggacgaaa cacaaggcga atagcgaact ggttggacaa ggactgggga
    39361 atatcatcgc tccgttcttt ggtggtatta ccgctacagc tgccatcgcg cgttctgccg
    39421 ctaacgtccg tgccggggca acgtccccta tctcggcggt gatccactct attctggtta
    39481 ttcttgccct gctggtactg gcaccgctgc tctcctggct gccgctttcc gctatggcag
    39541 ccctgctgtt gatggtggcg tggaacatga gtgaagcgca taaagtggtc gacttgctgc
    39601 gtcatgcgcc gaaagatgac atcatcgtca tgctgctgtg catgtcgctg accgtgctgt
    39661 ttgatatggt tattgccatc agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc
    39721 gtatcgcacg tatgactcgc ctggcaccgg tagtcgtaga tgttccagac gatgtcctgg
    39781 ttctgcgcgt tattggcccg ctgttttttg ctgctgctga aggcttattc acggacctgg
    39841 agtcacgtct tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt ccggtacttg
    39901 atgctggtgg tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac
    39961 tgcgcgtgtg caacgtggaa ttccagcctc tgcgcactat ggctcgcgca ggcattcaac
    40021 cgatcccggg acgcctcgcg ttcttcccga atcgtcgcgc ggcgatggcg gatttataag
    40081 tacgagattg gccagtcagc gcaaactgac tggtcagcaa actgcattat ttgttagcta
    40141 tgacggcgat tatcgctacg gcggcaacgt ttgtcatagt ggcgaaccca ccagtaacgg
    40201 tcgcacacct gttcatgccc gccaacacgg gcacccgcca gccagagaat ggcaccgacg
    40261 aaaatactta gcactgcacc atgtgcgaaa tactggggca tattaaactg tggtaactgg
    40321 ttgaggattg aataccccac gccgaccacc attaccacca gacccaaccc catgagcacg
    40381 ttaccgagta acgaagcgtt tttgcgtttc atatgtcacc tccggaactt tctgggttgt
    40441 aacagggaat acccctcttc cttatgtgta aagtatagac aacaccctgt gggttatgtg
    40501 cgggcgtgat cacaattaca acccttattt caacaaaact ttacaaataa acgcctgaac
    40561 accttgactt tctaatagtc cactcaggca attattacgt tatctgcatt tgatacgcag
    40621 ttttttgtca agcggggccg caaccagtaa actacgcgcc agttatgtac acactcagga
    40681 caaaaaaacg tgacgattaa attgattgtc ggcctggcga accccggtgc tgaatacgcc
    40741 gcaacgcgac ataatgctgg tgcctggttc gttgacttac tggcagagcg tttgcgcgct
    40801 ccgctgcgcg aagaggctaa attctttggt tatacttcgc gagtcaccct tggaggcgaa
    40861 gatgtccgcc tgttagtccc gactacattt atgaatctca gcggcaaagc cgttgcggcg
    40921 atggccagtt ttttccgcat taatccggac gaaattctgg tggcccacga cgaactggat
    40981 ctgcctcctg gcgtcgccaa atttaagttg ggcggtggcc atggtggtca caatggactg
    41041 aaagacatca tcagtaaatt gggtaataac cctaactttc accgtttacg catcggaatc
    41101 ggtcatccgg gcgataaaaa taaagttgtc ggttttgtgt taggcaaacc gcctgttagt
    41161 gaacagaagt taattgatga agccatcgac gaagcggcgc gttgtactga aatgtggttt
    41221 acagatggct tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt
    41281 gtctgcggca tttttgccga gtgccgtgta taataggcaa agttatttcc atttctgcaa
    41341 tctgttagca ataacaggtt gattattaag atattaaggt gatttaaatc atgggattca
    41401 aatgcggtat cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac gcgctgacca
    41461 aagccggtat tgaagcggcc aactttccat tctgcaccat tgagccgaac acaggcgtcg
    41521 taccaatgcc tgaccctcgc ctggatcaac tggctgaaat cgtaaaaccg cagcgtacgc
    41581 ttcccacgac catggaattt gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg
    41641 aaggtctggg taaccagttc ctgactaaca tccgtgaaac cgaagcgatc ggtcacgttg
    41701 ttcgctgctt tgaaaatgac aacatcattc acgtttccgg caaagttaac ccggcggacg
    41761 atattgaagt tatcaacacc gaactggcgc tggcagacct cgacacctgc gaacgtgcga
    41821 ttcatcgcgt acagaagaaa gccaaaggtg gcgataaaga cgcgaaagct gagctggcgg
    41881 tcctggaaaa atgcctgccc cagctggaaa acgcaggtat gctgcgcgcg ctggatttaa
    41941 gcgctgaaga gaaagcggct attcgttacc tgagcttcct gacgctgaaa ccaacaatgt
    42001 acatcgccaa cgtcaacgaa gacggttttg aaaacaaccc atatcttgac caggtgcgtg
    42061 aaatcgcggc gaaagaaggt tctgttgtgg ttccggtttg tgctgctgtt gaagcagaca
    42121 ttgccgaact ggacgacgaa gaacgtgacg agtttatgca ggagcttggg ctggaagagc
    42181 cgggcctgaa ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa acttacttca
    42241 ccgctggggt gaaagaagtg cgtgcatgga ccattccggt tggagcaacc gcgccgcagg
    42301 cagcgggcaa aatccatact gattttgaaa aaggctttat ccgtgcacaa accatctcgt
    42361 ttgaagattt catcacttac aaaggtgaac aaggcgcgaa agaagcaggc aaaatgcgtg
    42421 cagaaggtaa agattacatc gttaaagatg gcgatgtgat gaacttcctt ttcaacgtct
    42481 aattaatttg ttgtctcatg aggtttcgct aaatctcatg aagagcgcaa aagtatataa
    42541 aatccacgca attgcgtgga tttttcattt caccgagtcc taatcattct tgtaacatcc
    42601 tacacttttg tgcttattgc ctacagtgaa ccaccagaat aattaaagac atcagtgttc
    42661 agatgacgga tatctcatta gtagtgatat aacgtaagtt tttgttccac tacacatcac
    42721 ccccttcaca gctgatcaat attgatcacg ctccaatatg ctggcgagaa aaggacttac
    42781 ctgaagggta atatcccgca gataaagtcc ccgaataatg agattacgtg cgaacctggc
    42841 aagatgaaaa tgataaccag tagcacaata acaacgatag gccagaaaac ccattgacca
    42901 aaaatgaata tcgatgtttt ttgctttttc ttattagtaa tgcaaaaatg gcgcatattg
    42961 tttttgttat gcagatacat caagcgaaaa ccccataact aattaggatt attacaaaaa
    43021 atcttcacca aattttttaa ccgctgtttg ttgtaatatt tttgccatat tcatagtaac
    43081 gaattggaat aagtaacgac aacttaggga ataactcgat ctaaactcac ctttgtaagg
    43141 gggatgttaa atatatatta attcctgtgt tcctgatttt gcccggtaat agagaattat
    43201 tctcatagca gctcatcatg attgcaccat acaaatatca tgatggtaag aggatattat
    43261 tcattgactt aaacaaaatg acaaaatcca tagacccata gctgggtact atataggagt
    43321 ggtatgggca tcaaacaaca caatgggaat accaaagccg atcgtctcgc tgaattaaaa
    43381 atccgttcgc cctcaattca actgataaaa tttggcgcta ttggtttgaa tgcaattatc
    43441 ttttcccccc tgctgatagc tgctgataca ggaagtcaat atggcaccaa tattactatt
    43501 aatgatggtg acagaattac aggagatacc gccgatccat caggaaacct ctatggtgta
    43561 atgaccccag caggaaacac gcctggcaat atcaacctgg gtaatgatgt caccgtcaat
    43621 gtcaacgacg cctctggata tgcaaaagga atcattattc agggcaaaaa cagctccctg
    43681 acagctaacc gactcacagt agatgttgtt ggtcaaacct ctgccatcgg cattaattta
    43741 attggtgact atacccatgc tgacttaggc acaggcagca ccattaagag taacgatgac
    43801 ggcatcatta ttgggcatag ctcaacacta acagccactc aattcaccat tgaaaactcg
    43861 aacggtatag gcctaaccat caatgactat ggcaccagtg tcgatcttgg aagcggaagt
    43921 aaaatcaaga ccgatggaag tacaggtgtt tatatcggtg gtctcaacgg caataacgcc
    43981 aatggtgctg cgcgttttac ggcgacagac ctgacaatcg atgttcaggg ctacagcgcc
    44041 atggggataa acgtacagaa aaactcggtt gtcgatctcg gaacaaacag ttccattaaa
    44101 accagtggcg ataatgcaca cggcctctgg agctttggcc aggttagcgc gaatgcactc
    44161 actgttgatg taattggagc cgcggccaat ggcgtcgaag ttcgtggtgg tacaaccact
    44221 atcggtgcag atagccatat ttcttccgcg cagggcggtg gcctcgtcac cagtggttca
    44281 gacgcgacaa tcaatttttc tggcacggca gcgcaacgaa acagcatctt ttccggcggt
    44341 tcttatggtg cctcggccca gacggcaacg gctgttatca acatgcaaaa taccgatatt
    44401 acggttgatc gtaatggcag tctggcgctg ggtttgtggg cgctcagcgg cggtagaata
    44461 accggagaca gtttggctat caccggcgcg gcaggagcca gggggattta tgccatgacc
    44521 aacagccaga tcgacctcac gagcgatctg gtcattgata tgagtacacc cgaccagatg
    44581 gccatcgcaa cgcaacatga cgatggttat gccgccagcc gcatcaacgc ttcgggtcgt
    44641 atgcttatca acggtagcgt tctctccaaa ggtgggctaa tcaatctgga tatgcaccca
    44701 gggtcggttt ggacaggttc ctcccttagc gataatgtca atgacgggaa actggacgtt
    44761 gcaatgaata acagcgtctg gaacgtaaca agtaattcta atctcgacac gctggcgctg
    44821 agccattcaa ctgtcgattt tgccagccac gcatcaactg ccggcacatt taccacatta
    44881 aacgtagaga acctgagcgg taacagtacc tttattatgc gtgctgatgt tgttggcgag
    44941 ggtaatggcg ttaataataa aggggattta ttgaatatca gcgggagtag tgctggtaat
    45001 catgtattgg ctatccgtaa ccagggcagc gaagccacaa cgggaaatga agttctgaca
    45061 gtggtaaaaa ccactgacgg tgcggcctcg ttcagcgcgt cttctcaggt tgagttgggg
    45121 ggatatctgt acgatgtgcg taaaaatggc actaactggg agctttacgc ttccgggaca
    45181 gttccggaac cgactcctaa tcctgaacca acaccggctc ccgctcagcc tcccatagtc
    45241 aaccccgatc ctacgcctga acccgatcct acgcctaccc ccactcccac acctaaaccc
    45301 accacgaccg cagatgctgg cggcaattat ctcaatattg gttacttatt gaactatgtt
    45361 gaaaaccgta cgctgatgca acggatgggt gacctgcgaa atcagagtaa agacggtaat
    45421 atctggctgc gcagttatgg gggaagcctg gactccttcg ccagtggcaa actgagcggc
    45481 tttgacatgg gttacagcgg tatccagttt ggtggggata aacgtctctc tgatgtaatg
    45541 ccgttgtatg tcggtctgta tattggctca acacatgcat cgccggacta tagcggtggc
    45601 gacggtaccg cacgttcaga ctacatggga atgtacgcca gttacatggc acacaacggt
    45661 ttttacagcg atctcgttgt aaaagcatcg cgccagaaaa atagtttcca cgtacgggac
    45721 agtcagaaca acggcgttaa cgccaacggc actgcgaatg gattgagcat ctccctggaa
    45781 gccgggcaga ggttcaacct gacccctact ggttatgggt tctatataga gccgcaaacc
    45841 cagctcacat acagccacca gaatgagatg gctatgaagg cgagtaatgg tctcaatata
    45901 catctgaatc actacgaatc gctgctgggg cgtgccagca tgatactggg atatgacatc
    45961 accgcaggta aaagccagct gaatatgtat gtgaagactg gcgctatcca cgagttttca
    46021 ggggatacag aatatctgtt gaacaactcc cgggagaagt acagtttcaa aggtaatggc
    46081 tggaataacg gcgtgggagt cagtgcacag tataacacac agcacacatt ctacctcgaa
    46141 gcggattaca cgcagggtaa cctctttgat cagaagcaag tcaacggagg atatcgcttc
    46201 agcttttaag tgcgggatag acaagccctt aaacgatgct gaaaaatgtt agcgtgcgcc
    46261 ggaatgaaat atagatcact ctgatgtatc agaaatcgtt tctgtctttc atacccggcg
    46321 cttaaactgc cctgcatcaa tgccatgttg cttcattttc cgccacagcg tagtgcggcc
    46381 gattccaagt aaagccgaca tttcctgaat gcgaccgcct gtgacctggg ctgcgttaat
    46441 aattgcctct ttttcaactt ccgcaaatga cagactggtg gaaaggcgtg tggcgctgac
    46501 atcatctgtc gcctgctcgg taaacagatg ttccggcaaa tcactgacgc gaatgcgccc
    46561 attatcacta ctcagagcaa gattctcgat gacactgtaa agttcaaaat cgtttcctgg
    46621 ccatgcacaa gaaaccaggc gagcgagggc atcgtcatca attttcagcc gcgtagagaa
    46681 gcgtttttca agactgcgta atttgttatt caccaacgcc ggaatgctgc cacgccgcat
    46741 acgcagaggc gggatggtaa tttcaaatgc atgcagcgcg taatacagct ggcgactaaa
    46801 acgattttgt tccaccagca ttgcgaggtc cgcggtggtt gtagcaatca ctttgacatc
    46861 aattggtatt aaacgccgcg catccagtcg cgtgataaca ccctgcttga taacctgaag
    46921 caaagcagac tgtaactcca ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc
    46981 gtgcgccagt tccagccgac tcagacggcc attttcattg tccgtgcgat cgccgccaat
    47041 aaattcttcc gccagcgccg catcaccata taactcacaa ttgacggcga tataaggacc
    47101 tgcagcacgc tcgctttcat tatgaattgc ctgacttagc agtgccttgc ccacgccctc
    47161 ttctccacaa agcaggacag gaaagctact gcgcgccgcc tggcgaccaa aatgaatcaa
    47221 gcggcgggtt tgcggatcgt cctgtggcat atgagcgaag gtatggctga cttttcctaa
    47281 ttgactggtc atcaactgcc gcatctgttc cacaggatgg agcaacaaaa taaaactggt
    47341 tccctgcgtt tctattatcg gtttaagggt tatcaccgca tcaataaact ggtgctgact
    47401 ttcaaaggtt gcttctacgt gtttgagcgg atgtgcctgt tttattgctt gttgcaatac
    47461 ggcgggtaac gttaagagtt cagtgattgc ccgtccctga cttgccgtcg cgtcaaggcg
    47521 caagacccgc gccgcctggg cattaataaa ttgcaaatta ccctgctcgt cccagctaat
    47581 cacgccatca tccatacttt ctaacagggc attaagttga tttaaatgac ggttagtttc
    47641 agcgagcaaa ctgtccgtca gcagtaaatt tccgacctcg cgggcgattg ccaacgtcaa
    47701 cggcaaatca gctgcggtag tttgctcaac cggacacgca agcgctattg ttcccgtcaa
    47761 tcggcccttg ctgtcaaaca acggcgttgc acaaaaggcc cagttccaga gtgcctgttt
    47821 gaaatgttga tcggccatcg ttttcacggc ctgaccagag atagccgcta acgatagcgc
    47881 acaagtacca ataattccct cggcgcaata cgtgccgtca tggaatccaa gtgcacttag
    47941 ctgctgcaag gtttgcggat cgccattacg gctgagaatg caggcggttt catcgaggat
    48001 aaacagcgca cactctcgcg gtgccatata ttcccaggca tcttccagcg cagcctgccc
    48061 gagcgtcagc atcgctttct tacgccgata aatagaagca aatgtcacgc cctgggcctg
    48121 atgtggtacg ttccatgtct cccgtttcat cagcttattg catcgctccc aggaggttgc
    48181 aattaaggga gatatgcccc gaccatcgtt gttaaaagcg ccactcatat cccgcatccc
    48241 ttatgacaaa aggtgttccg gctcgcaatt ttaagtatta atacacgtaa aagtttcaat
    48301 atggaacatt cattaaagca tatgtttcac tatgaaacat aatttgggct aattttcttt
    48361 tcccttgcct gatgcacaat ggattcatcg gcagtcagtg gtcgccgtgt cgttgaacat
    48421 catccatgcc ctaccgtaat tgctggagca aaataatgaa aaaattgatc aatgatgtgc
    48481 aagacgtact ggacgaacaa ctggcaggac tggcgaaagc gcatccatct ctgacactgc
    48541 atcaggatcc ggtgtatgtc acccgagctg atgcccccgt tgcaggaaaa gtcgccctgc
    48601 tgtcgggtgg cggcagcgga cacgagccga tgcactgtgg ttatatcggt caggggatgc
    48661 tttcgggggc ctgtccgggc gaaattttca cctcaccgac gcccgataaa atctttgaat
    48721 gcgccatgca aattgatggc ggcgaaggtg tactgatgat tatcaaaaat tacaccggcg
    48781 atattcttaa ctttgaaaca gcgaccgagt tactgcacga tagcggcgta aaagtgacca
    48841 ctgtggtcat tgatgacgac gttgcggtaa aagacagtct ttatactgcc gggcgacgcg
    48901 gcgttgccaa caccgtatta attgaaaaac tcgtaggcgc agcggcggag cgtggcgact
    48961 cactggacgc ctgtgcggaa ctggggcgta agttgaataa tcaaggccac tcaataggta
    49021 tcgctctcgg tgcctgtacc gttcctgccg cgggcaaacc ttcttttacc ctggcggata
    49081 atgagatgga gtttggcgtc ggcattcatg gtgagccggg tattgaccgc cgccccttct
    49141 cttcccttga tcaaaccgtc gatgaaatgt tcgacaccct gctggtaaat ggctcatacc
    49201 atcgcacttt gcgtttctgg gattatcaac aaggcagttg gcaggaagaa caacaaacca
    49261 aacaaccgct ccagtctggc gatcgggtga ttgcgctggt taacaatctt ggcgcaactc
    49321 cgctttctga gctgtacggc gtctataacc gcctgaccac acgttgccag caagcgggat
    49381 tgactatcga acgtaattta attggcgcgt actgcacctc actggatatg accggtttct
    49441 caatcacctt actgaaagtt gatgacgaaa cgctggcact ctgggacgcc ccggtccaca
    49501 ccccggccct taactggggt aaataaggag aaagcaatgt cactgagcag aactcaaatt
    49561 gttaactggc tcactcgttg tggcgatatt ttcagcaccg agagcgagta tcttaccgga
    49621 ctggatcgcg aaattggcga cgctgaccac gggctaaata tgaaccgagg ctttagcaaa
    49681 gtggtggaaa aactccctgc tatcgcagat aaagatatcg gtttcattct caagaatacc
    49741 ggtatgacgc tgctttccag cgtcggtggt gccagtggtc cgctgttcgg taccttcttt
    49801 atccgcgccg cacaggcgac ccaggcacgg caaagcctga cactggaaga gctttatcag
    49861 atgttccgcg atggcgcgga cggcgtaatc agtcgcggga aagccgaacc tggcgataaa
    49921 accatgtgtg atgtgtgggt gccggtggtg gaatcgttac gtcagtccag cgagcaaaat
    49981 ctctctgttc cggtggcgct cgaagctgcc agtagaatcg ccgaatccgc tgcacaaagt
    50041 acgattacga tgcaagcccg caaaggccgc gccagttatc tcggtgaacg cagtattggt
    50101 caccaggatc ccggcgcgac ctcggtgatg tttatgatgc aaatgttggc gttagccgca
    50161 aaagagtaag gaattggtga tggtaaacct ggtcatagtt tcacatagca gccgactggg
    50221 agaaggtgtc ggtgaattag cccgtcagat gttaatgagt gatagttgta aaatcgccat
    50281 tgccgcggga attgacgatc cacaaaatcc tattggtacc gatgccgtca aagtgatgga
    50341 ggccatcgaa tctgttgctg atgccgacca tgtgctggtc atgatggata tgggtagcgc
    50401 attattgagt gctgaaactg cgctggaatt gctggctccc aagatcgccg caaaagtacg
    50461 tttgtgtgct gcgccgttgg tcgaaggtac actggcagca acggtcagcg cggcctcggg
    50521 agcggatatc gacaaagtta tctttgacgc tatgcatgcg ctggaagcca aacgtgaaca
    50581 actgggttta ccgtcctccg acactaaaat ctctgacaca tgtcctgcgt acgatgaaga
    50641 agcccgttct ctggcggtgg tcataaaaaa ccgtaacggc ctgcatgtac gtccggcctc
    50701 ccggctggtt tataccttat cgacatttaa tgccgatatg ttgctggaaa aaaacggcaa
    50761 atgcgtcaca ccagagagta ttaaccagat tgcgttacta caagttcgct ataacgatac
    50821 gctgcgcctg attgcgaaag ggccagaagc tgaagaggca ctgatcgctt tccgtcagct
    50881 ggctgaagat aactttggtg aaacggagga agtcgctcca cctactctgc gtcccgttcc
    50941 gcctgtttcg ggtaaagcct tttattatca accagtttta tgtacggtac aggcaaaatc
    51001 aaccctgacc gtggaagaag aacaagatcg attacgccag gctattgact tcacgttatt
    51061 agatctgatg acgttaacag cgaaagcaga agccagcggg cttgacgata ttgccgcaat
    51121 cttttctggt caccatacac tgttagatga tccggaactg ctggcggcgg caagcgaact
    51181 ccttcagcat gaacattgca cggcagaata tgcctggcag caagttctta aagaacttag
    51241 ccagcaatac cagcaactgg atgatgaata tctacaagct cgctatattg atgtggacga
    51301 tcttctgcat cgcaccctgg tccacctgac ccaaacgaaa gaagaactcc cgcagtttaa
    51361 ctcgccaact attctactgg cggagaacat ttatccttcc acagtactgc aactggatcc
    51421 ggcggttgta aaaggtatct gccttagcgc cggaagtccg gtatcccaca gcgccctaat
    51481 cgcccgtgag ctggggattg gctggatttg ccagcagggt gagaaactgt atgcgataca
    51541 accagaagaa acgctaacgc tggacgttaa aacgcaacgt ttcaaccgtc agggttaagc
    51601 cactgttcgg atggcatcgt tctgatgtca tccggcaatt tactcagcac taatggtttt
    51661 taactacctg ttatattttt caccaattta tgatgatttt tcttcgctaa tcacgaaccc
    51721 aacgccgctg gctggctaat aaacagacgt tatttgatca ctctatttcg ccgttattta
    51781 tccctttaat tcccttttct aaaatgcctg acagttcgca gaatgagatt tcgatcatgc
    51841 agctagtgcg atcctgaact aaggttttct gatacttgaa taccgtttta ttcggtttcg
    51901 ccaaaggaga atgattgatg aaatcccccg caccttctcg cccgcaaaaa atggcgttaa
    51961 ttccagcctg tatctttttg tgtttcgctg cgctatcggt gcaggcagaa gaaacaccgg
    52021 taacaccaca gccgcctgat attttattag ggccgctgtt taatgatgtg caaaacgcca
    52081 aactttttcc ggaccaaaaa acctttgccg atgccgtgcc gaacagcgat ccgctgatga
    52141 tccttgctga ttatcggatg cagcaaaacc agagcggatt tgatctgcgc catttcgtta
    52201 acgtcaattt caccctgccg aaagagggcg agaaatatgt tccgccagag ggacagtcac
    52261 tgcgcgaaca tattgacgga ctttggccgg tattaacgcg ttctaccgaa aacaccgaaa
    52321 aatgggattc tctgttaccg ctgccggaac cttatgtcgt gccgggcgga cgttttcgcg
    52381 aggtatatta ctgggacagt tacttcacca tgttaggact tgccgaaagc ggtcactggg
    52441 ataaagtcgc ggatatggtg gccaattttg ctcatgaaat agacacttac ggtcatattc
    52501 ccaacggcaa ccgcagttac tatttaagcc gctcgcaacc gcccttcttt gccctgatgg
    52561 tagagttact ggcgcagcat gaaggcgatg ccgcgttgaa gcaatacctg ccgcaaatgc
    52621 aaaaagaata tgcttactgg atggacggtg ttgaaaacct gcaagccgga caacaggaaa
    52681 aacgcgttgt aaaacttcag gatggtaccc ttctcaaccg ctactgggac gatcgcgata
    52741 cgccacgacc agagtcatgg gtggaagata ttgccaccgc caaaagcaat ccgaatcgac
    52801 ctgccactga aatttaccgc gacctgcgct ctgccgctgc gtctggctgg gatttcagct
    52861 cgcgctggat ggacaacccg catcaactaa ataccttacg caccaccagc atcgtaccgg
    52921 tcgatctgaa cagcctgatg tttaaaatgg aaaaaatcct cgcccgcgcc agcaaagctg
    52981 ccggagataa cgcgatggca aaccagtacg aaacgctggc aaatgcccgt caaaaaggga
    53041 tcgaaaaata catgtggaac gatcaacaag gctggtatgc cgattacgac ctgaaaagtc
    53101 ataaagtgcg caatcagtta accgcggccg ccctgttccc gctgtacgtc aatgcggcag
    53161 cgaaagatcg cgccaacaaa atggcgacgg cgacgaaaac acatctgctg caacccggcg
    53221 gcctgaacac cacgtcggtg aaaagtgggc aacaatggga tgcgccaaat ggctgggcac
    53281 cgttacagtg ggtcgcgaca gaaggattac aaaactacgg gcaaaaagag gtggcgatgg
    53341 acattagctg gcacttcctg accaatgttc agcacaccta tgaccgggag aaaaagctgg
    53401 tggaaaaata tgatgtcagc accaccggaa cggggggcgg cggtggcgaa tatccattac
    53461 aggatggctt tggctggacc aatggcgtga cgctgaaaat gctggatttg atctgcccga
    53521 aagagcaacc gtgtgacaat gttccggcga cgcgtccgac cgttaagtca gcaacgacgc
    53581 aaccctcaac caaagaggca caacccacac cttaaccagc gcttactccg acaacgtcat
    53641 tcggcttctc cggatggcgt tgtcggcaga taacaccata gctatggttt tttctacaca
    53701 gacggttttt caagtcctgg cgggaagtat tgcgcaaatg tttgccatac gtggttgccg
    53761 aagatagcgt catttctacg ggtaagtgtt tcactttcat cacccggcga tttacgccga
    53821 cgttggctat ataaagcatc aattgctacc tgaatattgt tacggtgttc actcatcaac
    53881 gtttttttgc tttctggagt taaattaata atcacataat ccagacactc cagagaattt
    53941 agcaaccaaa aaaagaattc agaccattcc tcttcgcgta actgaagcat tgccgaacgg
    54001 gtattttggc ttaatctgcc agaaggattg atcaattcta aatgttcaat aagcgcagcc
    54061 aaaaccgcct cactatccgc cccattttta acgcctgact ggctactgtc taaaatattt
    54121 attttctgaa tcacaccaaa aacaacgaaa caataattaa aaaaggattc tgttgttgtt
    54181 accgggaacc gactttatcg gatatcgaag tgctatattg atttttttca cccgtaaagc
    54241 aggtgtcgag tatacgttct gatgtgaaag cggatagcgg catctgtatc gctatccgcc
    54301 ttatgaaaat cttattctct tcgcaggagg cggaatatgc ccagaacgag aatagctccc
    54361 accaccgcca ccaggaagct gtgcagatta aaaccactga tggagccgcc aatgccaaac
    54421 atggtcgcca gccagccgcc gaccaccgca ccgactatcc cgagaataca ggtcaggaaa
    54481 aatccaccac catcacgccc cggcatgatt agcttggcga taatgccggc tatcaggcca
    54541 aaaataatcc aggcaataat tcccatgttc aggccctcgt ttcgatgaat cggacgcaag
    54601 aaatgcttac gtcaacgctc taagtatagg caagggcggg atcgatgctg ggcgtatgtc
    54661 ggctgattta agaacaacat gtaaaaaata ttcacgcatt tgtattaagt tttgttaact
    54721 gtgaccgata aaccaaagac agtttgtcag tcaggagttt ttccgcgtga gtcattacca
    54781 tgagcagttc ctgaaacaaa atccgttagc cgtcctgggc gtgttacgcg atttgcacaa
    54841 agccgcaatt cctttgcgtc tcagttggaa tggcgggcag ctgatcagca aaatactggc
    54901 aataaccccg gataaactgg tgctggattt cggcagtcaa gccgaagaca acatcgccgt
    54961 gctaaaggca cagcacatta ccattaccgc cgaaactcag ggtgcgaaag tcgagtttac
    55021 tgttgaacaa ctacagcaga gtgaatactt gcagcttccg gcatttatca ccgtaccgcc
    55081 tcccacctta tggtttgtac aacgacgccg atatttccgc atctccgccc cactccatcc
    55141 gccttatttt tgccagacca aactggcgga taacagtacg ttacgtttcc gcctgtatga
    55201 tttgtcgtta ggcggcatgg gcgcattact ggaaacagca aagcctgccg aattacaaga
    55261 aggcatgcgc ttcgctcaga ttgaagtcaa catggggcaa tggggtgttt ttcactttga
    55321 cgcccagtta atctccatca gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac
    55381 cactccccgt ctgagcttcc gttttcttaa cgtcagcccg acggtggagc ggcaattaca
    55441 gcggattatt ttctctctcg agcgagaagc ccgggaaaaa gcggacaaag tgcgcgactg
    55501 atttacatcg cgtccagtgc ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca
    55561 ggatgatttc gcgctacgtg atcgaggaac tcgtcagcat ccaaatcgtt gattttgctg
    55621 atcgcctttt tccgatctga cgagaaagtc cgtagcagcg cacctgcccc gttagcgtat
    55681 gacaccacca gcgcatattg cagtaccttc ggatcttcaa tgcctgccag cgggccggtt
    55741 tccagaatat tcaggtaagc cgcccccatt gaaatattac gctctggatt tttcagctcg
    55801 ctggtcgtcg gctcaccact ccagcccata cggcgataaa catcacgtcc ggaggttgaa
    55861 gcttttaact gcatcaaacc aatggcattc gatttactca ccgcattcgg attaccaccc
    55921 gattcgatag cgataatcgc cgtgatcaat tgtggatcga cgccccaggc tgcaccggct
    55981 ttctggctta ttggcatcca ctgcatcgca cgttgcaccg gaactttcgc gttccacggc
    56041 gggttcgtat agtcatgctt tgatgaacaa cccgctaata acacaatcaa aaaggcaaac
    56101 catctcaatt tcacttcttc tatccttata gcctggaatc gccgttgagg tgacaacggc
    56161 acacctaagc ggcatgatat acatttcgtt tctgttgtca gcaaggaatt gccatgtctc
    56221 tgtttcactt aattgcccca tcgggttact gcattaaaca gcacgccgcg ctgcgtggta
    56281 tccagcgcct taccgacgag gggcatcagg tcaataacgt cgaggttatt gcccgtcgtt
    56341 gtgagcgttt tgcgggtacg gaaacggagc gcctggaaga tctcaattcc cttgccagac
    56401 tgaccacccc caacaccatc gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac
    56461 tggcagatat tgactggcag gcactggttg ctcgccaaca atatgacccg ttgctcatat
    56521 gcgggcatag cgattttacc gccattcagt gcggtcttct ggcgcaaggc aatgtcatca
    56581 cctttagcgg ccccatgctg gtagcaaact ttggcgcgga tgaactgaac gcctttaccg
    56641 agcaccattt ctggctggcg ttacgcaatg aaaccttcac cattgaatgg caaggggaag
    56701 gcccgacatg tcagaccgaa ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac
    56761 tgattggtac accgtggatg ccaaaaattg agaacggtat tctggtgctg gaagatatta
    56821 acgagcaccc tttccgcgtc gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc
    56881 cacgtcagaa ggcgattatt ctcggtagct ttagcggcag cacgcccaat gattatgacg
    56941 caggttacaa cctggagtca gtgtacgcgt ttttgcgttc ccgcctgtcg attccactca
    57001 ttaccggcct cgattttggt catgaacaac gcacggttac gctgccgttg ggcgcacatg
    57061 ccattctgaa taacacccgg gaaggcactc agttgacgat tagcggtcat cctgttctta
    57121 aaatgtaaaa aaatcacgct tcagggctaa gtaaaacgct gtctctgccg ctaatatggt
    57181 agggtttata taagagacag cgtaatcagg agtaaccgac cgttggatgc cacaacaata
    57241 attagccttt tcatcttagg atccatcctt gtcaccagca gtatattact tagttcattt
    57301 tcttcccgtc ttggcattcc tattctggtt atttttctgg cgatcggcat gctggcagga
    57361 gtcgatggcg tcggcggtat cccgtttgat aattacccct tcgcctacat ggtaagtaac
    57421 ctggcactgg cgattatttt gctcgacggc gggatgcgca ctcaggccag ttcctttcgt
    57481 gtggcgttag gaccggcact gtcgctggcg acgctgggcg tgcttatcac ctctggttta
    57541 accggcatga tggcggcgtg gctgtttaat cttgatttga ttgaaggctt attaatcggc
    57601 gctatcgtcg gctctaccga tgctgcagcg gtcttttctc tgctgggtgg taaggggctt
    57661 aacgaacgtg ttggctcgac gctggaaatt gaatccggca gtaatgatcc aatggcggtc
    57721 tttctgacga ttaccctgat tgcgatgatc cagcaacatg aaagcagtgt cagttggatg
    57781 ttcgtggtcg atattctgca gcaatttggc ctcgggattg tcatcggact tggcggtggt
    57841 tatttactgc tgcaaatgat taatcgcatc gccctgcctg ccggattata tccattgctg
    57901 gcattaagcg gcggtatttt aatttttgct ttaaccacgg cgctggaagg cagcggtatt
    57961 ctggctgttt atctgtgcgg ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc
    58021 atcctgcaaa atttcgacgg cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg
    58081 gggctattgg ttaacccaag cgatctgctg cctattgcca ttccggcgct cattttgtcg
    58141 gcatggatga tattcttcgc ccgtcccctt tcggtatttg ctggattgct ccccttccgc
    58201 ggtttcaatc tgcgtgagcg cgtgtttatc agctgggtag gattacgcgg cgcggtgcca
    58261 atcatcctgg ccgtgttccc gatgatggcg gggctggaga atgcacgtct gttctttaat
    58321 gtcgccttct ttgtggttct ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg
    58381 gctaaaaaag ccaaagtggt ggttccgcca gtgggacgtc cggtgtcacg cgttggcctg
    58441 gatattcatc cggaaaatcc gtgggagcag tttgtttatc aattgagtgc cgataaatgg
    58501 tgcgtgggcg cggcactgcg cgatttgcat atgccaaaag agacgcgtat tgcggcactg
    58561 tttcgtgata accagttgct tcatcctacc ggcagcaccc gactgcgcga aggcgatgtg
    58621 ttgtgtgtaa ttggtcggga acgcgatctt ccggcgctcg gtaaactgtt cagccagtcg
    58681 ccgccggtcg cgctggatca acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa
    58741 tatgctgatg ttgcgctgat atatggcctg gaagacgggc gagaatatcg tgataagcag
    58801 caaacgctgg gtgaaattgt ccagcagttg ttaggcgcgg caccggttgt cggtgaccag
    58861 gtagagtttg ccgggatgat ctggacggtg gccgagaaag aagataatga agtgttgaag
    58921 attggcgtac gggtggctga agaggaagct gaatcttaaa tttaatgaag catctatcgc
    58981 cggttgcgat gctttgctga acgcagcctg agcctgcaat tgtttgtcgt ccataattta
    59041 accttcattt gatgttgaat tgaacatatc aaaatcaggc aattacacaa atctatgtac
    59101 aggctccatc aagggtaaag cgtgatttat ctgaagtcga ctccgagtca acaatgaagc
    59161 gtaagcgttt tatgtagaac gcctgtgcgg tgagttcagg ttccggggaa atagtgtcca
    59221 tccttttaat ggacactatc atgggcaatg gctctgtcac attaacaact acagttgctg
    59281 accagccgga tggatgattt tttcgcaacc agaagacgct gcctccgatc cggcttacaa
    59341 caagttacac tgtcacaacc gggacgcgta acgccagcgc gcacatcaac tcatagccca
    59401 ccgttccggc agcggcggcg acatcatcaa ttttgatctc cttgccccac agctcaaccg
    59461 gcgtaccaat acccgcctgc gggcaaggcg ttaaatcgac cgccagcata tccatcgaga
    59521 cggtccccac cgtcatggtg cgcacgccgt ccactaaaac aggggtaccg gtaggcgcgt
    59581 ggcgcggata accgtcggcg taccctgcgg cgacaatgcc gattcgctgt tcatcgcgcg
    59641 cagtatagcg accgccgtag cccacacgct cacccgcttt tagcgtctgg acaccaataa
    59701 tctcactgct tagagtcatc accggacgta atccggtatt ggcgatatca cgccactgac
    59761 cggacggcga agcgccatac aaaataatgc caggccgaac ccagtcaaaa tgcgcttccg
    59821 ggtgccacag agtcgccgcc gaattggaca acgaacgccg acactccagc ccttccgccg
    59881 cctgctcaat acgcgccatc gcgctggaaa ttccatcagg atgttccgct tcggcaaaat
    59941 gcgacatcag ggtcatctcg ccaacattcg ccattgcccg caactgctgc cagacggtaa
    60001 gcacgcgatc gggctggaag cccaaccgat tcatcccact gttcacttta agataaatat
    60061 ccaacggtgc ttttagccgc gcattttgca gtgctttgag ctgccagttg ctgtgtacgc
    60121 aggtggtcag gcggtgctgg tcataaatct ccagatcctg agcatggaaa aatccttcca
    60181 gcatcaggat cggccccttc cagccgcgct cacgtaacgt tattgcctct tccaggttaa
    60241 gtaatgcaaa gccatcggtg gccccgagcg cgctccagat acgctcaata ccatgcccgt
    60301 aagcgttcgc ttttaccacc gaccagacgc gcgcgtgcgg cgcggcctgg cggacaatgg
    60361 acagattctg ttttaatgcc tgcagatcga ggctggcctg tatcggacgg gtcatctcgt
    60421 ttccttagct gtgtgcgcca tgtaaatggc ccggacgtga tggcgtaaat ccacggctgt
    60481 agcgcgctac gcttagatcc tcatatggga tcgctggcgt gcgaccagag agcagatcgc
    60541 ttaacaactg accggaaccg caagccatcg tccagccgag cgtgccgtga ccggtattca
    60601 gccacagatt tttaaagcgt gtacgcccga caaccggcgt accgtctggc gtcatcgggc
    60661 gcaaacccgt ccagaaagtc gcctgctcga catgaccgcc gcgtggatag agatcgcgaa
    60721 ccaccatttc cagcgtttca cgacgcggtt gcaacagctc ggtgttaaaa ccaacaattt
    60781 ccgccattcc gccaacacga atgcggttat cgaaacgggt aatggcgatt ttgtaggttt
    60841 catcaagaat agtggatact ggcgcaccat cttcctgcgc aattggaata gtcagcgagt
    60901 agcctttcag cggatacacc ggaatatcaa caatgccttt gagcatcgcc gtcgagtaag
    60961 aaccaaacgc catcacatac gcatcggcct taatcacttc atcgccacac ttcacgccgt
    61021 agatttgctc gccgtcgcaa agcagttggt caacgggcgt attaaagcgg aatttaaccc
    61081 ccgcctgctc tgccatccgc gccagattct gggtaaacag ctggcagtct ccggtttcat
    61141 cattgggcag ttgcagacca cccgtcagtt tgtgcgctac ttctgccagc gcgggctcca
    61201 cttccgccag gcggctggat tccagcagct gatacggtac gccggcatct tccagcacgg
    61261 cgatatcgcg ggtcgcattt tcatactgtt gttcggtacg gaacagttgc agcgtcccac
    61321 cctgacgccc ttcatactga atattggttt cggcgcgcaa tgctttcagg caatcacggc
    61381 tgtattccgc cagacgcacc atccgccctt tgttttccat gtagtggctg gtgtcgcagt
    61441 tacgtaacat ttgccacatc catttcaact gaaactgcgt accgtcgaga cggaccgcca
    61501 gcggcgcatg gcgctggaac atccatttga tcgcttttaa aggcacacct ggtgccgccc
    61561 acggtgcagc atatccgggg gagatttgcc cggcattcgc ggcgctggtt tccagggcgg
    61621 cccccggctc ccgatcaatg acggtgacct catgtcctgc ctgatttaag taccaggcgc
    61681 tggcaacgcc taccacacca cttcccagta tgacaactcg catagccact ccgataacgg
    61741 tagatacagt aaaagaataa taatctaatt acatcttgat aactcagttg aaaatattat
    61801 tcaacatacg gcttttttat ggtgagctgg ctcacatctc cctgactctg cggcaatatg
    61861 gcgtctttag tatctcctgc tgaacgccct ttttatccag cataaaattc cgttcagaag
    61921 cggattagtg gcactctgat aataagaaat cgcaaagaaa aaaattctcc ggcgacgcgg
    61981 tttttaacag gtgttctatg cttgaaatga ggtgctccac acagagagcg ccgacaacaa
    62041 tgaggtgcgc gtatggcgac gatcgattct atgaataagg acaccacacg tttgagcgat
    62101 ggacccgact ggacgttcga cctgctggat gtttatctgg cagagataga ccgggtggcg
    62161 aaactctacc ggctggatac ctacccgcac cagattgaag tgataacctc agaacagatg
    62221 atggatgcct actccagcgt cggcatgcca attaactatc cgcactggtc attcggtaaa
    62281 aagtttatcg agactgaacg gctgtataag cacggtcagc aaggactggc ctatgaaatc
    62341 gtcattaact ctaacccgtg tatcgcttac ctgatggaag agaacaccat taccatgcag
    62401 gcgctggtga tggcacacgc ctgctatggg cataactctt tctttaaaaa caattactta
    62461 ttccgcagtt ggaccgacgc cagttcgatt gtcgattatc tgatttttgc ccgtaaatat
    62521 attaccgagt gcgaagaacg ttatggcgtt gatgaagtag aacggcttct ggactcgtgc
    62581 cacgcgctga tgaactacgg cgtggaccgg tacaaacgcc cgcaaaaaat ctcgctgcaa
    62641 gaagagaaag cccggcagaa aagtcgcgaa gagtatctac aaagtcaggt caatatgctc
    62701 tggcgtaccc tgccgaagcg cgaggaagag aaaacggttg ctgaagcgcg ccgctatccg
    62761 tccgaaccac aagaaaacct gctctatttt atggagaaaa atgcgccact gctggaatca
    62821 tggcagcgtg aaatcctgcg tattgtgcgt aaggtgagcc agtattttta tccgcaaaaa
    62881 cagactcagg tgatgaacga aggctgggcg accttctggc actataccat ccttaaccat
    62941 ctgtatgatg aagggaaagt aacggaacgt tttatgctgg agtttttgca cagccacacc
    63001 aatgtggtct tccagccccc ctataacagc ccgtggtaca gcggcatcaa cccgtatgcc
    63061 ttcgggttcg ccatgttcca ggatattaaa cggatttgtc agtcgccaac ggaagaagac
    63121 aaatactggt tcccggatat cgccggttcc gactggctgg aaacgctgca tttcgcgatg
    63181 cgtgatttca aagatgagag ttttatcagc cagtttctgt caccgaaagt gatgcgtgat
    63241 ttccgcttct tcaccgtgct ggatgatgat cggcataatt atctggagat ttccgctatt
    63301 cataatgaag aaggttatcg ggagatccgt aaccggttat cgtcgcaata taacttaagt
    63361 aatctggagc cgaatattca gatctggaac gtggatttgc gcggcgaccg ttcgctgacg
    63421 ctgcgttaca ttccacataa tcgcgcaccg ctggatcggg ggcgcaaaga agtgctgaag
    63481 catgtgcatc gcctgtgggg atttgatgtg atgctcgaac agcaaaacga agacggcagc
    63541 atcgagttgc tggaacgttg cccgccaaga atgggaaatc tgtaaaaaca acaaaaaacc
    63601 cctcgtttga ggggtttgct ctttaaacgg aagggattat cgcccctgaa tggctaaatc
    63661 acccggcaga tttttctgca tccggtgcca aatctcgcca ctctcatgcc catagcgacg
    63721 cactgtttcg tacacctgat cgtgcgcgcc ttcactgcac aacgccgaca gtttgtggta
    63781 gaagcccagc gccagactgc gcgcttccgg attggcgaaa tagtgacgac caatgcgcgt
    63841 atacagccct ttcatcccgt taaggatcag accgtaaatc gggttgccgg aagcaaacgc
    63901 caggccgcgg aatatgttgt aatccagctc ggcaaaggca tcggcgtgat cggccacttc
    63961 attagcggta gccagcactt cctgcgcttt atcgggatgc tgacgaaacg cggtgcgaat
    64021 aaaaatagtg gaaatattgg tacgcaccga cagcaaatta tcaataagct gcggcacact
    64081 ttcgtgatcc agtcgcgcca gtgtttcaag gatatttaaa ccagaggttt cccagaaatt
    64141 attcaccttc gtcggcttgc catgttgaat ggtcaaccag ccatctcgtg ccagacgctg
    64201 taacacttca cgtaacgtag tacgcgttac gccaattaat tctgaaagtt cacgttctgc
    64261 gggcaaaata gtcccgggag ggaagcggtt attccagata ctttcaataa tgtactcttc
    64321 cgcgaaaccc gccgggcttt gcgccttaat gaccatagtg agatttccat aacacagcaa
    64381 aacaaagttg gactcatcat accagagggc taactacgct gatagcagac aaaaagaaga
    64441 aaaaagggat caaggcttca ttttccgcat ctctttcccc ttgactatca agacttgcct
    64501 cactcctgcc tgaccgttaa gctttgcgtt taaacataaa aacatcattg attatttgta
    64561 aggtaaggga aaccatcatg gagatctcct ggggccgcgc gctatggcgc aattttttgg
    64621 gccagtcccc cgactggtac aaactcgccc tcatcatttt cttaatcgta aacccgttaa
    64681 ttttcctcat cagccctttc gtcgcgggct ggttgctggt agcggaattt attttcactc
    64741 tggcgatggc cctgaaatgc tacccgctgc tccccggtgg tctgttggct atcgaagcgg
    64801 tattcatcgg catgaccagc gcggaacacg tccgtgaaga ggtggcggca aatcttgaag
    64861 tcttgctgtt attgatgttt atggtggcgg gtatctattt tatgaaacag ctgttgctgt
    64921 tcatatttac ccgtttgctg ttaagcattc gctccaaaat gctgctgtcg ctctcttttt
    64981 gcgtggcggc agcgttcctc tccgcgttcc tcgatgcctt aaccgtcgtg gcggtggtga
    65041 tcagcgttgc tgtcggtttt tatggtattt atcaccgcgt agcctcttcc cgtaccgaag
    65101 ataccgacct gcaagacgat agtcatatcg acaagcatta caaagtggtt ctggaacagt
    65161 tccgtggctt tctgcgtagc ctgatgatgc atgccggtgt cggcactgca ttaggcggcg
    65221 tgatgaccat ggtgggcgaa ccacagaacc tgatcatcgc taaagcggct ggctggcatt
    65281 ttggcgattt cttcctgcgc atgtcgccgg tgaccgttcc ggttctgatt tgtggcctgt
    65341 taacctgcct gctggtagag aagctgcgtt ggtttggcta cggtgaaacg ctgccggaga
    65401 aagtccgcga agtgttgcaa cagtttgacg atcaaagccg ccaccagcgt acccgtcagg
    65461 ataaaatccg tctgattgtc caggcgatta ttggcgtctg gctggtgact gcgctggcgt
    65521 tgcatctggc ggaagttggc ttgattggtt tgtcagtcat tattctggcg acatcattga
    65581 ccggtgtcac cgatgaacat gctatcggta aagccttcac cgaatctctg ccattcaccg
    65641 cactgttgac ggtgtttttc tcggtagtcg cggtgattat cgaccaacaa ctgttttcgc
    65701 caattattca gtttgtgttg caggcatcgg aacatgctca gctgtcgctg ttctatattt
    65761 tcaacggtct gctgtcatcc atttcggata acgtcttcgt ggggacgatt tatatcaacg
    65821 aagcgaaagc ggcaatggaa agtggcgcta tcacgttgaa gcaatacgag ctgctggcgg
    65881 tcgccattaa taccggtacc aatctgccct ccgtcgctac gccgaacggt caggctgcgt
    65941 tcctgttcct gctgacctct gcactcgcgc cattgattcg cctctcttat ggccgcatgg
    66001 tgtggatggc cctgccttac accctcgtac tgacactcgt cggcttgctc tgcgtcgagt
    66061 ttacgcttgc ccctgtaacc gaatggttta tgcaaatggg ctggatagca acgctttgat
    66121 aacaacttac cgggcatata aatgcccggt ttgccttttc gcccgataat tgtccaattg
    66181 cttcctattt ttgctgcctc ctggtggcgc agcgaatgaa ttggtttaaa ctgcgcactc
    66241 tatgcatatt gcagggaaat gattatgttg cgatttttga accaatgttc acaaggccgg
    66301 ggcgcgtggc tgttgatggc gtttactgct ctggcactgg aactgacggc gctgtggttc
    66361 cagcatgtga tgttactgaa accttgcgtg ctctgtattt atgaacgttg tgcgttattc
    66421 ggcgttctgg gtgctgcgct gattggcgcg atcgccccga aaactccgct gcgttatgta
    66481 gcgatggtta tctggttgta tagtgcgttc cgcggtgtgc agttaactta cgagcacacc
    66541 atgcttcagc tctatccttc gccgtttgcc acctgtgatt ttatggttcg tttcccggaa
    66601 tggctgccgc tggataaatg ggtgccgcaa gtgtttgtcg cctctggcga ttgtgccgag
    66661 cgtcagtggg attttttagg tctggaaatg ccgcagtggc tgctcggtat ttttatcgct
    66721 tacctgattg tcgcggtgct ggtggtgatt tcccagccgt ttaaagcgaa aaaacgtgac
    66781 ctgttcggtc gctaatccct tcagcgctcc tgcgggagcg cttttttcct gccgctatat
    66841 ttatttgacc cgcagtaaat cagaacttcg cgttgtataa cgtggtgaaa gcatggcacg
    66901 cttcatctgc cattgttgct ggatcccctg cccggcaaaa tagagtgttc ctctgccctc
    66961 tttagcattc agtgtatcca ttaccgtcat caattgctca ctcccggggc gcggtgcgtt
    67021 gtcatcgaat aaattgagct gcgcgactcc ctgactgaag aaatccccca gcatcacgcc
    67081 cgctttttgg tagcgatggc ccgcttgcca gatggcatcc agagatcgcg tagcagcgtt
    67141 aatgatatcc ctgctgtcct gagtgggcgt cagcagtttt accgacgcgc tattgccgta
    67201 ataaggttca ttgagcgcaa atggtgacgt cttaataaat gtggagataa accgacaata
    67261 ttgatgctcg ctgcgaagtt tttccgccgc ccgggcagcg taactacaaa tggcctgccg
    67321 catcgatgga taatccgtga tgcgttcacc aaacgagcgg gaacagataa tttcctgctt
    67381 cgtcggtgca aactcttcca gttgcaaaca gggttcgccg cgcagttcac gcaccgttct
    67441 ttcgagcacg acattaaaat gtttacggat aaaccggata tctgtatccg ccaaatcgag
    67501 aacggttttg atccccatcg cgtccagttt tttgctgatc cgccgtccaa tcccccagac
    67561 gtcatccacg gggagagcag acattaattt acgctggcgt tccagatttg ataaatccac
    67621 caccccaccc gtctgccgct gccatttttt tgccgcatga ttggcaagct tagccaacgt
    67681 tttggtctgg gcgatcccca caccaacagt aagatgggta cgttgtagca ccgttgcgcg
    67741 aatttctctg ccaaaatcag tcagatcgcg acaattacgc acacctgtca gatcgcagaa
    67801 tgcctcatca atactgtaaa tctcgacgcg gggcgatagt tcttccagcg tcgacatcac
    67861 ccgattgctc atgtctgcgt aaagctcata attgctgcta aagcaaacca cgccacagcg
    67921 acgaaacaga tctttttgtt tgaaccaggg atcgcccatt ttaacaccca gcgcctttgc
    67981 ctcagcgttt cgggcgataa cgcaaccgtc attattcgat agcacaacca ccggtttacc
    68041 ccataaatca gggcgaaaca ccgtctcaca gctggcataa aacgcgttta catcacagag
    68101 agcaaacatc agcgcatcgc cttaacgacg tggatcacca caccaaagac atccagcgta
    68161 tcttcgctac tgatggtaat gggcgagtac gcactgttca tgggaataag ctgtaccgtc
    68221 gggcgtagtt gcaatttttt caccgtaaac tcgccgtcaa cagcagcgat gacaatatca
    68281 ccatggctgg cggtaatagc gctatcgaca atcagtaaat caccgtcact aattccacca
    68341 tcaatcatag aatcaccact tgctttgacg aagtaagtcg cgctgggatg ctggatcaac
    68401 agttgattca gatcgatgcg ctgttcaacg taatctgctg ccggtgaagg aaagccacac
    68461 tgaacaagat cgctaaatag cggaaaagtc acaatttcgc ggagatccgc aggcttgata
    68521 aacaacataa taatctgcct gaagttatac tgtttttata tacagtagtc tgttcttgcc
    68581 agcagatcaa tactgattca ggctatcaat atttgtcgct gcatagactg ctgatttttc
    68641 gttctcttat cctgtgctca cgtggccttc tggcgacgac gctcatccag cagaaatgaa
    68701 aaatatcacc cggcaaaaaa atagaataga agcattcgcc ataatgacat taaacattgt
    68761 ttgatattta tcatattaat agaaataaag aca
//
LOCUS       DAMZII010000006        67756 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZII010000006 DAMZII010000000
VERSION     DAMZII010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.006"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            131..466
                     /gene="eutS"
                     /locus_tag="POA77_000342"
     CDS             131..466
                     /gene="eutS"
                     /locus_tag="POA77_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDI6363838.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            479..958
                     /gene="eutP"
                     /locus_tag="POA77_000343"
     CDS             479..958
                     /gene="eutP"
                     /locus_tag="POA77_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDI6363839.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            933..1634
                     /gene="eutQ"
                     /locus_tag="POA77_000344"
     CDS             933..1634
                     /gene="eutQ"
                     /locus_tag="POA77_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDI6363840.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1631..2434
                     /gene="eutT"
                     /locus_tag="POA77_000345"
     CDS             1631..2434
                     /gene="eutT"
                     /locus_tag="POA77_000345"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDI6363841.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2431..3447
                     /gene="pta"
                     /locus_tag="POA77_000346"
     CDS             2431..3447
                     /gene="pta"
                     /locus_tag="POA77_000346"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6363842.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3486..3779
                     /gene="eutM"
                     /locus_tag="POA77_000347"
     CDS             3486..3779
                     /gene="eutM"
                     /locus_tag="POA77_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDI6363843.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3886..4173
                     /gene="eutN"
                     /locus_tag="POA77_000348"
     CDS             3886..4173
                     /gene="eutN"
                     /locus_tag="POA77_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDI6363844.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4185..5588
                     /locus_tag="POA77_000349"
     CDS             4185..5588
                     /locus_tag="POA77_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDI6363845.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5599..6435
                     /gene="eutJ"
                     /locus_tag="POA77_000350"
     CDS             5599..6435
                     /gene="eutJ"
                     /locus_tag="POA77_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDI6363846.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDVGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6425..7612
                     /gene="eutG"
                     /locus_tag="POA77_000351"
     CDS             6425..7612
                     /gene="eutG"
                     /locus_tag="POA77_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDI6363847.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7729..8955
                     /gene="eutH"
                     /locus_tag="POA77_000352"
     CDS             7729..8955
                     /gene="eutH"
                     /locus_tag="POA77_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDI6363848.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8952..10355
                     /gene="eutA"
                     /locus_tag="POA77_000353"
     CDS             8952..10355
                     /gene="eutA"
                     /locus_tag="POA77_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDI6363849.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10367..11728
                     /gene="eutB"
                     /locus_tag="POA77_000354"
     CDS             10367..11728
                     /gene="eutB"
                     /locus_tag="POA77_000354"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDI6363850.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11749..12636
                     /gene="eutC"
                     /locus_tag="POA77_000355"
     CDS             11749..12636
                     /gene="eutC"
                     /locus_tag="POA77_000355"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDI6363851.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12646..13305
                     /gene="eutL"
                     /locus_tag="POA77_000356"
     CDS             12646..13305
                     /gene="eutL"
                     /locus_tag="POA77_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDI6363852.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13318..13818
                     /gene="eutK"
                     /locus_tag="POA77_000357"
     CDS             13318..13818
                     /gene="eutK"
                     /locus_tag="POA77_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDI6363853.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13864..14916
                     /gene="eutR"
                     /locus_tag="POA77_000358"
     CDS             13864..14916
                     /gene="eutR"
                     /locus_tag="POA77_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDI6363854.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCQGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14922..15821)
                     /gene="hemF"
                     /locus_tag="POA77_000359"
     CDS             complement(14922..15821)
                     /gene="hemF"
                     /locus_tag="POA77_000359"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDI6363855.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15825..16694)
                     /gene="amiA"
                     /locus_tag="POA77_000360"
     CDS             complement(15825..16694)
                     /gene="amiA"
                     /locus_tag="POA77_000360"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDI6363856.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16908..17333
                     /locus_tag="POA77_000361"
     CDS             16908..17333
                     /locus_tag="POA77_000361"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDI6363857.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17320..17769
                     /locus_tag="POA77_000362"
     CDS             17320..17769
                     /locus_tag="POA77_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDI6363858.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17830..18405
                     /locus_tag="POA77_000363"
     CDS             17830..18405
                     /locus_tag="POA77_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDI6363859.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18501..19400
                     /gene="yfeX"
                     /locus_tag="POA77_000364"
     CDS             18501..19400
                     /gene="yfeX"
                     /locus_tag="POA77_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDI6363860.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19458..20762)
                     /gene="pbp4b"
                     /locus_tag="POA77_000365"
     CDS             complement(19458..20762)
                     /gene="pbp4b"
                     /locus_tag="POA77_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDI6363861.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGNKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20767..22191)
                     /gene="murP"
                     /locus_tag="POA77_000366"
     CDS             complement(20767..22191)
                     /gene="murP"
                     /locus_tag="POA77_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDI6363862.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22195..23091)
                     /gene="murQ"
                     /locus_tag="POA77_000367"
     CDS             complement(22195..23091)
                     /gene="murQ"
                     /locus_tag="POA77_000367"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI6363863.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23255..24112
                     /gene="murR"
                     /locus_tag="POA77_000368"
     CDS             23255..24112
                     /gene="murR"
                     /locus_tag="POA77_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDI6363864.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24241..25032
                     /gene="ucpA"
                     /locus_tag="POA77_000369"
     CDS             24241..25032
                     /gene="ucpA"
                     /locus_tag="POA77_000369"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDI6363865.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25190..26206
                     /gene="cysP"
                     /locus_tag="POA77_000370"
     CDS             25190..26206
                     /gene="cysP"
                     /locus_tag="POA77_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDI6363866.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26206..27039
                     /gene="cysT"
                     /locus_tag="POA77_000371"
     CDS             26206..27039
                     /gene="cysT"
                     /locus_tag="POA77_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDI6363867.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27039..27914
                     /gene="cysW"
                     /locus_tag="POA77_000372"
     CDS             27039..27914
                     /gene="cysW"
                     /locus_tag="POA77_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDI6363868.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27904..29001
                     /gene="cysA"
                     /locus_tag="POA77_000373"
     CDS             27904..29001
                     /gene="cysA"
                     /locus_tag="POA77_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDI6363869.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29135..30046
                     /gene="cysM"
                     /locus_tag="POA77_000374"
     CDS             29135..30046
                     /gene="cysM"
                     /locus_tag="POA77_000374"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDI6363870.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30049..30417)
                     /locus_tag="POA77_000375"
     CDS             complement(30049..30417)
                     /locus_tag="POA77_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDI6363871.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30522..31373
                     /gene="pdxK"
                     /locus_tag="POA77_000376"
                     /gene_synonym="thiJ"
     CDS             30522..31373
                     /gene="pdxK"
                     /locus_tag="POA77_000376"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDI6363872.1"
                     /translation="MSSLLLFNDKSRALQADIVSVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31415..31924)
                     /gene="crr"
                     /locus_tag="POA77_000377"
     CDS             complement(31415..31924)
                     /gene="crr"
                     /locus_tag="POA77_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6363873.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31965..33692)
                     /gene="ptsI"
                     /locus_tag="POA77_000378"
     CDS             complement(31965..33692)
                     /gene="ptsI"
                     /locus_tag="POA77_000378"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDI6363874.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33737..33994)
                     /gene="ptsH"
                     /locus_tag="POA77_000379"
     CDS             complement(33737..33994)
                     /gene="ptsH"
                     /locus_tag="POA77_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDI6363875.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34378..35349)
                     /gene="cysK"
                     /locus_tag="POA77_000380"
     CDS             complement(34378..35349)
                     /gene="cysK"
                     /locus_tag="POA77_000380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI6363876.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35534..36295)
                     /gene="cysZ"
                     /locus_tag="POA77_000381"
     CDS             complement(35534..36295)
                     /gene="cysZ"
                     /locus_tag="POA77_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDI6363877.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36525..37511
                     /gene="zipA"
                     /locus_tag="POA77_000382"
     CDS             36525..37511
                     /gene="zipA"
                     /locus_tag="POA77_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDI6363878.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37582..39597
                     /gene="ligA"
                     /locus_tag="POA77_000383"
     CDS             37582..39597
                     /gene="ligA"
                     /locus_tag="POA77_000383"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI6363879.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39599..39817
                     /locus_tag="POA77_000384"
     CDS             39599..39817
                     /locus_tag="POA77_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDI6363880.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39814..40812)
                     /locus_tag="POA77_000385"
     CDS             complement(39814..40812)
                     /locus_tag="POA77_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDI6363881.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40902..41828
                     /locus_tag="POA77_000386"
     CDS             40902..41828
                     /locus_tag="POA77_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6363882.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQFE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41819..42160)
                     /locus_tag="POA77_000387"
     CDS             complement(41819..42160)
                     /locus_tag="POA77_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDI6363883.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42280..42405)
                     /locus_tag="POA77_000388"
     ncRNA           complement(42280..42405)
                     /ncRNA_class="other"
                     /locus_tag="POA77_000388"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(42436..42511)
                     /locus_tag="POA77_000389"
     tRNA            complement(42436..42511)
                     /locus_tag="POA77_000389"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42476..42478),aa:Lys,seq:ttt)
     gene            complement(42516..42591)
                     /locus_tag="POA77_000390"
     tRNA            complement(42516..42591)
                     /locus_tag="POA77_000390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42556..42558),aa:Val,seq:tac)
     gene            complement(42638..42713)
                     /locus_tag="POA77_000391"
     tRNA            complement(42638..42713)
                     /locus_tag="POA77_000391"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42678..42680),aa:Val,seq:tac)
     gene            complement(42758..42833)
                     /locus_tag="POA77_000392"
     tRNA            complement(42758..42833)
                     /locus_tag="POA77_000392"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42798..42800),aa:Val,seq:tac)
     gene            43092..44507
                     /gene="gltX"
                     /locus_tag="POA77_000393"
     CDS             43092..44507
                     /gene="gltX"
                     /locus_tag="POA77_000393"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI6363884.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(44559..44951)
                     /locus_tag="POA77_000394"
     CDS             complement(44559..44951)
                     /locus_tag="POA77_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI6363885.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(44953..45312)
                     /locus_tag="POA77_000395"
     CDS             complement(44953..45312)
                     /locus_tag="POA77_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI6363886.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45533..45608
                     /locus_tag="POA77_000396"
     tRNA            45533..45608
                     /locus_tag="POA77_000396"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45566..45568,aa:Ala,seq:ggc)
     gene            45648..45723
                     /locus_tag="POA77_000397"
     tRNA            45648..45723
                     /locus_tag="POA77_000397"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45681..45683,aa:Ala,seq:ggc)
     gene            45932..48121
                     /locus_tag="POA77_000398"
     CDS             45932..48121
                     /locus_tag="POA77_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDI6363887.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(48171..49373)
                     /gene="nupC"
                     /locus_tag="POA77_000399"
     CDS             complement(48171..49373)
                     /gene="nupC"
                     /locus_tag="POA77_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDI6363888.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            49709..50947
                     /locus_tag="POA77_000400"
     CDS             49709..50947
                     /locus_tag="POA77_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI6363889.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(51088..51414)
                     /locus_tag="POA77_000401"
     CDS             complement(51088..51414)
                     /locus_tag="POA77_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDI6363890.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEVTLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(51529..52785)
                     /locus_tag="POA77_000402"
     CDS             complement(51529..52785)
                     /locus_tag="POA77_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDI6363891.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQQ
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHSMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            52989..53954
                     /gene="glk"
                     /locus_tag="POA77_000403"
     CDS             52989..53954
                     /gene="glk"
                     /locus_tag="POA77_000403"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDI6363892.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            54173..54499
                     /locus_tag="POA77_000404"
     CDS             54173..54499
                     /locus_tag="POA77_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDI6363893.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            54521..55768
                     /locus_tag="POA77_000405"
     CDS             54521..55768
                     /locus_tag="POA77_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6363894.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            55783..56868
                     /gene="ypdF"
                     /locus_tag="POA77_000406"
     CDS             55783..56868
                     /gene="ypdF"
                     /locus_tag="POA77_000406"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6363895.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            56868..57905
                     /gene="ypdE"
                     /locus_tag="POA77_000407"
     CDS             56868..57905
                     /gene="ypdE"
                     /locus_tag="POA77_000407"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6363896.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRTVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            57930..60425
                     /gene="ptsP"
                     /locus_tag="POA77_000408"
     CDS             57930..60425
                     /gene="ptsP"
                     /locus_tag="POA77_000408"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6363897.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIILTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(60428..61285)
                     /locus_tag="POA77_000409"
     CDS             complement(60428..61285)
                     /locus_tag="POA77_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6363898.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(61298..62032)
                     /gene="ypdB"
                     /locus_tag="POA77_000410"
     CDS             complement(61298..62032)
                     /gene="ypdB"
                     /locus_tag="POA77_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDI6363899.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(62047..63744)
                     /gene="ypdA"
                     /locus_tag="POA77_000411"
     CDS             complement(62047..63744)
                     /gene="ypdA"
                     /locus_tag="POA77_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDI6363900.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            64121..65359
                     /gene="alaC"
                     /locus_tag="POA77_000412"
     CDS             64121..65359
                     /gene="alaC"
                     /locus_tag="POA77_000412"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDI6363901.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(65424..65495)
                     /gene="ypdK"
                     /locus_tag="POA77_000413"
     CDS             complement(65424..65495)
                     /gene="ypdK"
                     /locus_tag="POA77_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDI6363902.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(65851..66771)
                     /gene="lpxP"
                     /locus_tag="POA77_000414"
     CDS             complement(65851..66771)
                     /gene="lpxP"
                     /locus_tag="POA77_000414"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDI6363903.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            67124..67366
                     /locus_tag="POA77_000415"
     CDS             67124..67366
                     /locus_tag="POA77_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDI6363904.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(67443..67718)
                     /gene="ypdI"
                     /locus_tag="POA77_000416"
     CDS             complement(67443..67718)
                     /gene="ypdI"
                     /locus_tag="POA77_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDI6363905.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT        15958 a        17092 c        18617 g        16089 t
ORIGIN      
        1 atgcaccaca gcgaatacct ctccattctt agtgatctac ctcacctttt aaacgcgctt
       61 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
      121 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
      181 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
      241 tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga tcgccggtga
      301 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
      361 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
      421 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
      481 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
      541 gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg ataagggcga
      601 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
      661 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
      721 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
      781 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
      841 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
      901 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
      961 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
     1021 ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
     1081 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
     1141 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
     1201 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc
     1261 agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat ggcagcagcg
     1321 tcaagtttgg tcgtttcgac ggcgcagagc cgcattgcgt gggcttgact gatcttgtga
     1381 ccggagacga cggcagcagc atggccgctg gttttatgca gtgggagaac gcattcttcc
     1441 cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctg catgttcgcc
     1501 acgaaggcga aaccatgatc gccaaagcgg gtgacgtgat gtttatcccg aaaggctcca
     1561 gtatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg ccggctaact
     1621 ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc atacgctcag
     1681 cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg cccgggagtt
     1741 actggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc tgtttgttga
     1801 cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg aacatccgca
     1861 ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc tgactcacct
     1921 gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga
     1981 cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt ggcagccgtg
     2041 gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc tgggtgaacc
     2101 gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcgac tttctcacca
     2161 gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg ggcgcgatgc
     2221 ggcgttgctc aatctgctac gtaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt
     2281 gtttatcacc cgcagctttg aagtattacg cccggacatt ttgcaggcgc tgaaccgcct
     2341 ttccagcacg gtctacgtga tgatgatcct gagcgtaacg aagcagccgc taacggtgaa
     2401 gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg tgaactggcg
     2461 ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag accaacgtgt gctgaaagcc
     2521 gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgccaa tccgtttgaa
     2581 cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt gattgatccg
     2641 catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg cgcgggcgaa
     2701 aaaacgccgc cggatgcgct ggaaaaactt accgaccctc tgatgttcgc cgcagcaatg
     2761 gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac ggcaaatgtg
     2821 ctgcgtgccg gattacgcat tatcggcttg cagccaggct gtaaaacgct ctcttccatt
     2881 ttcctgatgt tgccacagta cagcggcccg gcgttgggct ttgccgattg cagcgtggtg
     2941 ccacagccga cggcggcgca gctggcggat atcgcgcttg ccagtgccga aacctggcgc
     3001 gccatcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagcaa tggtagcgcc
     3061 cgtcacccct gtgttgctaa cgtccagcag gcgacagaaa tcgtccgtga gcgcgcacca
     3121 aagctggtgg tggatggcga gttacagttt gacgccgcct tcgtgccgga agtggcggcg
     3181 caaaaagcgc ctgccagccc gctacagggc aaggccaatg tgatggtttt tccgtcgctg
     3241 gaagccggaa atattggtta caaaatcgca caacgactcg gcggatatcg tgccgtcggg
     3301 ccactgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg tagcgtgcag
     3361 gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt gaaccgcgaa
     3421 agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat aaagaggaaa
     3481 gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg attgaggctt
     3541 ctgacgcgat ggtgaaagct gcgcgcgtca agctggttgg tgttaagcag attggcggcg
     3601 gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg accgatgcag
     3661 gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc ccgcgtccgc
     3721 acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc aacctgtaat
     3781 caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt accttctgcg
     3841 gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa actggcagtc
     3901 gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga caaattgctg
     3961 atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt cgccatcgac
     4021 aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc cgcccgccag
     4081 gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt cgatgaggtg
     4141 gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat caacaggata
     4201 ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg ccgtccgccg
     4261 ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca gccaaagtcg
     4321 cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc attcgtgaag
     4381 caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc ggcatggggc
     4441 gcgttgaaga taaatttgca aaaaacgtcg ctcaggcgcg cggcacacca ggcgttgagt
     4501 gcctctctcc gcaagtgctg actggcgaca acggcctgac cctaattgaa aacgcaccct
     4561 ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta attaataacg
     4621 ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa
     4681 aagtctccca gcgggcgatt acgctgctca accaggcgat tgttgccgca ggtgggccgg
     4741 aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcaacgc ttgttcaagt
     4801 ttccgggtat cggcctgctg gtggtaaccg gcggcgaagc ggtagtagaa gcggcgcgta
     4861 aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta gtggtggatg
     4921 aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca
     4981 acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc gatgaactga
     5041 tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg cagcagctgc
     5101 aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc cgtgactggg
     5161 ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg caagaaacgc
     5221 gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc
     5281 cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac
     5341 tggagggcgg ttgccaccac acggcggcaa tgcactcgcg caatatcgaa aacatgaacc
     5401 agatggcgaa cgccattgat accagcattt tcgttaagaa cggaccgtgc attgccgggc
     5461 tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc ggtgaagggg
     5521 taaccagcgc gcggacgttt gtccgtctgc gtcgctgtgt attagtcgat gcgtttcgca
     5581 ttgtttaagg agtaaccgat ggcgcacgac gaacaatggc tcaccccacg tctgcaaacg
     5641 gcggcaacgc tgtgtaacca gacgcctgcc gcgacggaat ctccgctgtg gctgggggtg
     5701 gatctgggca cctgcgatgt ggtgtcgatg gttgttgacc gcgacggtca gccggtggcg
     5761 gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt cttcggtgcg
     5821 gtcaccattg ttcgtcgcca tctcgacact cttgagcagc aattcggtct tcgttttagc
     5881 catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa cgtgctggaa
     5941 tctgccgggc tggaagtgag ccatgtgctg gatgagccaa ccgcagtcgc ggatctgctg
     6001 caactggaca acgccggtgt agtggatgtc ggcggcggca ctaccggcat tgccatcgtg
     6061 aaaaagggca aggtgacgta ctcggcggat gaagccaccg gcggacatca catctctctg
     6121 actctcgccg gaaatcgtcg tatttcgctg gaagaggcag agcagtacaa gcgcggtcat
     6181 ggtgacgaga tttggcccgc ggtgaaaccg gtgtacgaaa aaatggcgga cattgtcgcc
     6241 cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc ttgtatgcaa
     6301 ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca tttaccgcag
     6361 cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa agcggagggg
     6421 ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc tgaatctgca
     6481 acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggct cggtgagcag
     6541 ttgcggacag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg cagacagctt
     6601 tttgcatcag gcagggatga ccgccgggct gacgcgtagc ctggccgtta aaggtatcgc
     6661 catgacgctc tggctatgtc cggtgggcga accgtgcatt accgacgtgt gtgcagccgt
     6721 ggcgcagttg cgtgagtcag gttgtgacgg ggtgatcgca tttggcggcg gctcggtgct
     6781 ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc tggcagagat
     6841 gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa ctaccgccgg
     6901 aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg ggcgcaagca
     6961 ggtgttagcc catgcctcgc tgatgccgga tgtggcgatc ctcgacgccg cattgaccga
     7021 aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc atgccattga
     7081 agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg gtgccattgc
     7141 gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg ccgcgcgcga
     7201 gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg gtcttgggtt
     7261 gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccgcacg gtctggcgaa
     7321 cgccatgttg ctgccaacag tgatggaatt taaccggatg gtttgtcgtg aacgctttag
     7381 tcagattggt cgggcactgc gaactaaaaa atccgacgat catgacgcta ttaacgcggt
     7441 aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg gcgcgacatc
     7501 tgcgcattac ggtgcatggg cgcaggccgc gcaggaagat atttgtctgc gcagcaaccc
     7561 gcgtaccgcc agcctggagc agattgtcgg cttgtacgca gcggcgcaat aaatgccgga
     7621 tgcgacgctt gccgcgtctg atccggcctt cgggattgca catgtagggc ggataaggcg
     7681 tttacgccgc atccgccaat aaataacaac taacagggag taaaggcgat gggaattaac
     7741 gaaatcatca tgtacatcat gatgttcttt atgctgatag ctgccgtaga caggatcctg
     7801 tcgcagttcg gcggttctgc tcgtttcctc ggtaagttcg gtaaaagtat cgaaggatct
     7861 ggcggtcagt tcgaagaagg ctttatggca atgggcgctc tgggtctggc gatggtcggt
     7921 atgaccgcgc tggcaccggt actggctcac gtactcgggc cggtaattat cccggtttac
     7981 gaaatgctcg gcgcaaaccc gtcaatgttc gccggaacgc tgctggcgtg cgatatgggc
     8041 ggcttcttcc tcgccaaaga gctggcgggc ggcgacgtag cagcgtggct atactctggg
     8101 ttaattcttg ggtcgatgat ggggccaacg attgtgtttt ccattccggt ggcgctcggc
     8161 attatcgaac cttctgaccg tcgttatctg gcgctcggcg tgctggcggg cattgtgacc
     8221 attccgattg gctgtattgc tggtggtctg gttgctatgt actccggtgt gcagatcaac
     8281 ggtcagccgg tggaattcac tttcgccctg atcctgatga acatgatccc ggtgatcatt
     8341 gtcgcgattc tggtggcgct ggggctgaaa ttcatcccgg aaaaaatgat caacggcttc
     8401 cagatcttcg ccaaattcct cgttgcattg atcaccctcg gtcttgccgc tgcggtagtg
     8461 aaattcctgc ttggctggga actgatcccc ggtctggatc ctatctttat ggcccctggc
     8521 gataaacccg gtgaagtgat gcgcgccatt gaagttatcg gttctatctc ctgcgtgctg
     8581 ttaggggcgt atccaatggt gctgctgctg actcgctggt ttgaaaaacc gctgatgagc
     8641 gtcggtaaag tactgaatat gaacaacatc gcggcagccg gcatggtggc aacgcttgcc
     8701 aacaacatcc cgatgttcgg catgatgaag cagatggata cccgcggcaa agtcatcaac
     8761 tgcgccttcg ccgtttccgc tgctttcgcc ctgggcgacc acttaggctt cgccgctgcc
     8821 aacatgaacg ccatgatctt cccgatgatt gtcggcaagt tgatcggcgg cgtaacggcg
     8881 attggcgtgg cgatgatgct ggtgccaaaa gaagacgcga ccgcgactaa aaccgaagcg
     8941 gaggcacaat cgtgaacact cgccagctat tgagcgtcgg tatcgatatc ggcaccacca
     9001 ccacccaggt gattttctcc cacctggagc tggttaaccg tgcggcggtg tcgcaggtgc
     9061 cgcgctacga attcattaaa cgcgaaatta gctggcaaag tccggtgttc tttacccctg
     9121 tcgataaaca gggcggttta aaagaagcgg aactgaaaac cttaatactc gagcaatatc
     9181 aggctgcggg tattgcgccg gaaagcgttg attctggtgc catcatcatc accggtgaaa
     9241 gcgcgaaaac ccgcaatgct cgcccggcgg tgatggcgct ctctcaatcg ctgggggatt
     9301 ttgtcgttgc cagcgccggg ccgcacctcg aatccgtgat cgccggtcac ggagctgggg
     9361 cgcaaaccct ttctgaacaa cggctgtgtc gggtactgaa tatcgacatc ggcggtggca
     9421 ccgcgaacta cgccctgttc gatgccggaa aaatcagcgg cactgcctgt ctcaacgtcg
     9481 gtggtcgcct gctggaaacc gacagccagg ggcgcgtggt ttacgctcat aaaccggggc
     9541 agatgattgt ggatgagtgc ttcggtgcag gcactgacgc ccgttcgctg accggcgcgc
     9601 agctggtgca ggttacccgg cggatggcgg cgctgattgt cgaagtgatt gacggaacgc
     9661 tttcgccgct cgcgcaggca ttgatgcaaa ccggtttgct gcccgcaggt gttacgcccg
     9721 aaatcattac gctttctgga ggcgtgggcg aatgttatcg ccaccagccc gccgacccgt
     9781 tctgttttgc cgatattggc ccacttctgg caacggcgct gcatgaccat ccgcgcctgc
     9841 gtgagatgaa tgtgcagttt ccggcgcaaa ccgtacgcgc cacggtgatt ggcgcgggtg
     9901 cacataccct ttcgctctct ggcagcacaa tctggctgga gggcgtacaa ctgccgctgc
     9961 gcaatttgcc ggtggcgatc ccgattgatg aaacggatct ggtgagtgcc tggcaacagg
    10021 cgctgcttca gctggatctt gatcccaaaa ctgacgcgta cgtgctggcg cttcccgcct
    10081 cgctgcctgt gcgttacgcc gcggtactga cggtcatcaa cgcgctggtc gatttcgtcg
    10141 cgcgttttcc gaatccgcat cccctgctgg tggtggccgg gcaggacttt ggtaaagctc
    10201 tgggcatgtt gttgcgccca cagctacaac aactcccgtt ggcagtcatt gacgaagtga
    10261 ttgtccgcgc gggggactat atcgacattg gtacgcctct ttttggcgga tcggttgtgc
    10321 cggtgacggt gaaatcactc gcatttcctt cctgagggaa cgacttatga aactaaagac
    10381 cacattgttc ggcaatgtat atcagtttaa ggatgtaaaa gaggtgctgg ctaaagccaa
    10441 cgaactgcgt tcgggggatg tgctggcggg cgttgcagcg gcaagctcac aggagcgcgt
    10501 ggcggcaaag caggtgttgt cggaaatgac cgtagcggac atccgcaata atccggtgat
    10561 tgcctatgaa gatgactgcg tgacgcggct gattcaggac gatgttaacg aaacggccta
    10621 caaccagatt aaaaactgga gcatcagcga actgcgtgag tatgtgctga gcgatgaaac
    10681 cagcgtggac gacattgcct ttacccgcaa agggctgacc tcggaagtgg tcgcggcggt
    10741 agcgaagatt tgctccaacg cggacctgat ctacggcgcg aagaaaatgc cggtaatcaa
    10801 aaaggccaat accaccattg gtattccggg caccttcagc gcccgtttgc agccaaatga
    10861 cacccgtgac gacgtgcaaa gtatcgccgc gcaaatctac gaagggcttt ccttcggggt
    10921 gggcgatgcg gtgatcggcg ttaacccggt gactgacgac gtggaaaact taagccgcgt
    10981 gttggacacc atttacggcg tgatcgacaa attcaacatc ccaactcagg gctgcgtact
    11041 ggcgcacgtc accacccaga tcgaagcgat tcgtcgcggc gcgcctggcg gactgatttt
    11101 ccagagtatc tgtggcagcg aaaaagggct gaaagagttt ggcgtggaac tggcgatgct
    11161 cgacgaagcg cgcgcagtgg gtgcagagtt caaccgtatc gccggggaaa actgcctcta
    11221 cttcgaaacc ggacaaggct ctgcgctatc cgctggcgct aacttcggcg ctgaccaggt
    11281 gacgatggaa gcacgtaact atgggctggc gcgtcattac gatccgttta tcgtcaacac
    11341 cgtggtcggc tttattgggc cggagtatct ctacaacgac cgccagatta tccgtgctgg
    11401 cttagaagat cactttatgg gcaagctgag cggcatctct atgggctgtg actgctgcta
    11461 caccaaccac gctgacgctg accagaacct caacgaaaac ctgatgatcc tgctcgccac
    11521 cgcaggctgc aactacatca tggggatgcc gctgggtgat gacatcatgc tcaactacca
    11581 gaccaccgca ttccacgata ccgccactgt gcgtcagtta ctcaacctgc gcccgtcacc
    11641 agagtttgaa cgctggctgg aaagcatggg cattatggca aacggtcgcc tgaccaaacg
    11701 ggcgggcgat ccgtcactgt tcttctgatg acgcggagat aacacatcat ggatcaaaaa
    11761 cagattgaag aaattgtacg cagcgtgatg gcgtcaatgg gacaaacggc ccccgcgccg
    11821 tcagaagcaa agtgcgccac caccacctgt gcggcaccgg tgacctcgga aagctgcgcg
    11881 ctggatttag gttctgcgga agcaaaagcg tggattggtg ttgaaaaccc gcatcgcgca
    11941 gacgtattaa cagaactgcg tcgcagcacc gtggcccgcg tttgtaccgg tcgtgccggt
    12001 ccgcgtccgc gtacgcaggc gttgctgcgt ttcctggccg atcactctcg ttcgaaagat
    12061 accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc agggcttgct ggaagtacgc
    12121 tccgaaatta gcgacaaaaa tctctacctg actcgcccgg atatgggccg ccgcctgtgt
    12181 gcagaagctg ttgaagcgct gaaagcacag tgtgttgcta atccggacgt acaggtcgtt
    12241 atttctgatg gcctgtcaac tgatgccatc accgtgaact acgaagagat cctgccgccg
    12301 ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca cgccattctt tgttcgttat
    12361 ggtcgcgtga agattgaaga tcagatcggt gagatcctcg gcgcgaaagt ggtgatcctg
    12421 ctggtggggg aacgtccggg gttagggcag tcagaaagcc tctcctgcta cgccgtttac
    12481 tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct gtatctctaa cattcaccag
    12541 ggcggcacgc cgccggttga agccgcagcc gtcattgtgg atttggcgaa acgtatgctg
    12601 gagcagaaag catccggcat caacatgacc cgataaggag gcatcatgcc agctttagat
    12661 ttgattcgac cgtcggtaac cgccatgcgg gtgattgcct ccgttaacgc tgaatttgcg
    12721 cgtgaactaa aattgccgcc gcatattcgt agcctcgggc tgatttctgc tgattccgat
    12781 gacgtcacct acattgccgc tgatgaagcg accaagcagg cgatggtgga agtggtatat
    12841 ggtcgctcgc tgtatgcggg cgctgcacac ggcccgtcac cgactgccgg tgaagtgctg
    12901 attatgcttg gtgggccaaa cccggcggaa gtgcgtgccg gtctggatgc gatggttgca
    12961 catattgaaa atggtgcagc attccagtgg gccaatgacg cagaaaacac cgccttcctg
    13021 gcgcatgtgg tttcgcgtac cggttcttat ctctcatcaa ccgccgggat cacgcttggc
    13081 gatccgatgg cgtatctggt ggcaccgccg ctggaagcga cctatggcat tgatgcagcg
    13141 ttgaaatctg ccgacgtaca gctggtgacc tatgtcccgc caccgtctga aaccaactac
    13201 tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag cagcctgtaa cgcctttacc
    13261 gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg cgtaacggag gttgccgatg
    13321 atcaatgcac tgggattgct ggaagtggac ggcatggtcg ccgcgataga tgcagcggat
    13381 gccatgctca aagcagctaa cgttcgtctg ctcagtcacg aagtgcttga ccctggtcgc
    13441 ttaacgctgg tggtggaagg cgatctggcg gcgtgtcgtg cggcgctgga cgcaggttgt
    13501 gctgccgcga tgcgtaccgg gcgtgtcatc agccgcaagg agatcggtcg gccagacgat
    13561 gacacccagt ggctggttac tggctttaac cgccagccga agcaacccgt aagggaaccc
    13621 gacgcgccag ttatcgtcgc ggaatctgct gacgagttgt tggcgctgtt aacatcagta
    13681 cgtcagggaa tgacggcagg agaagtggct gcccactttg gctggccgct ggaaaaagcc
    13741 agaaatgcgc tcgaacagct cttttctgcc gggacgttac gtaaacgcag tagtcgttat
    13801 cgtctcaagc cccattaacc tgtcggaggt gccgggtgtc ctacaacacc cggcattaac
    13861 atcatgaaaa agacccgtac agccaatttg caccatcttt atcatgaacc cttacccgaa
    13921 aacctgaagc tcacgccgaa ggtcgaagtg gataatgttc atcaacgaca gacaacggat
    13981 gtctatgaac atgctttaac gattaccgcc tggcagcaga tttacgatca gttgcatccg
    14041 ggcaagtttc atggtgaatt tacggaaatt ctactcgatg atattcaggt ttttcgtgaa
    14101 tacaccggtc tggcgctgcg tcagtcgtgc ctggtctggc cgaactcgtt ctggtttggc
    14161 attccggcga cgcgcggtga gcagggattt atcggttcgc aatgtcaggg aagcgcggaa
    14221 atcgccaccc gccctggtgg cactgaattt gaactgagca cgccggatga ttacacgatc
    14281 ctgggcgtgg tgctttctga agatgtcatc acccggcagg ctaacttttt gcataacccg
    14341 gatcgggtat tacatatgct gcgtagccag tcggcgctgg aagtgaaaga gcagcataaa
    14401 gccgcgctgt ggggctttgt ccaacaggcg ctggcgacgt tttgcgagaa cccggaaaat
    14461 ctccatcagc ctgcagtgcg aaaagtgctg ggggataatt tgctaatggc gatgggggcg
    14521 atgctggaag acgcgcaacc aatggtgacg gcggaaagca tcagtcatca gagttaccgt
    14581 agattacttt cccgcgcccg tgaatatgtg ctggaaaata tgtctgagcc ggtgacggtg
    14641 ctggacttgt gtaatcaact gcatgtcagt cgccgcacgc tacaaaacgc gtttcacgct
    14701 attttaggca ttggcccaaa cgcgtggctg aaacgcattc gcctgaacgc cgtacgccgc
    14761 gaactgataa gcccgtggtc gcaaagcaca acggtaaaag acgccgccat gcagtgggga
    14821 ttctggcatc tggggcaatt tgccacggat taccagcagc tgtttgccga gaagccgtcg
    14881 ttgacgttgc atcagcggat gcgggaatgg gggtgaggga gttacaccca atccctgacc
    14941 ttaataaact cacttaacgc cgcttctggg ctgccatctt ttggctgata atcatactcc
    15001 cagcgtacca gtggcggcat tgacatcagg atcgactcgg tgcgcccgcc agtttgcagg
    15061 ccaaacagag tgccgcgatc ccagaccaga ttgaactcga cataacgacc gcgacggtag
    15121 agctgaaaat tgcgctcgcg ctcgccgtag gccatcgctt tacgtcgctc tacaattggt
    15181 aaataagcgt cggtgtagcc tttgcctacc gcctgcataa aggcaaaaca gtggtcgaaa
    15241 tctggcgtgt tcagatcatc aaagaacagc ccgccaatac cgcgctgttc gttgcgatgt
    15301 ttgaggtaga agtaatcgtc gcaccacttt ttgtaacggg gataaacgtc ttcaccaaat
    15361 ggcaggcaca ggtcacgggc ggtgcggtgc cagtgaatgg cgtcttcttc aaaaccatag
    15421 aaaggggtta aatcgaagcc gccgccaaac caccagacgg gctctgcacc cggtttttcg
    15481 gcaataaaaa aacgcacatt cgcgtggctg gtgggaacat acgggttatg cggatgcact
    15541 accagtgaaa cgcccatcgc ctcgaaactg cgcccggcaa gctccgggcg atgagcggtg
    15601 gcggaagcag gcatcgcctc gccgtggacg tgagaaaagt tgacgcctgc ctgttcgaaa
    15661 acaccaccat tacgcaacac ccgactacgc ccgccgccgc cagcttcgcg ctgccaacta
    15721 tcttcgacaa attctgcgcc atcgacggcg gtcagctgct gacaaatcgt atcctgaagg
    15781 ttgagcagaa actgtttaac ctggtgtgcg tcgggtttca taacttatcg ctttttcgaa
    15841 tgtgctttct ggttgtcgaa ccagtggaaa taactgatca cgccttcagc aatcgctgtg
    15901 gcgattttct gacgaaacgc cgccgtgcct aacagccgct cttcttccgg gttggtgata
    15961 aacgaggttt ccaccagcac cgaaggaacc gacggcgatt tcaacaccac aaacgcggct
    16021 tgttcggtgt tgcggctgtg cagtttatgc accggcttaa tcttcttcag aatatgcgag
    16081 ccgagcgtca gactattttt aatggtatct gtttgcacca gatcaaacag cacttgttgc
    16141 aataggtgat ccttgtcagt cgccttttta ccggcaactt catcggcgcg gttttcgcgt
    16201 tcagacaggt atttcgccat tgcgctactt gccccacggt tagagagggc aaataccgaa
    16261 gcaccggcag ctttcgggtt ggtaaagcca tcggcatgaa ttgacataaa cagatctgcg
    16321 ccatgtttat gggcgatttc aacgcgatcg taaagtggga taaacgtatc gccagaacgc
    16381 gttaaacgcg catcaatccc atgattacgc aaaatggaac ggacgttttt agcaatcgcc
    16441 agcaccacat gtttttcttt cgaaccgttg cgtccgatcg ctccggtatc aattccgccg
    16501 tgacctggat cgagaacaac gacacgtttg ccgccagatt ttttggcttt cggcttgctg
    16561 tgtccgttgc tggtttttaa aagttcgtct ttggcgatgg cttgcgacat tcctgacaac
    16621 gtcagggcag ccaaaccggc tttcagcacc tggcggcgcg aagtgagtgt ttttagtggt
    16681 ttaaaagtgc tcatacggcc tgagttgtaa taataaagtt ccagatgtta tatcgtatcg
    16741 cgtaatccgt tacgacggtt tgatgttgag aattgtttta cttttcattt caatagttga
    16801 caactcccca ttatgccact ttttcaaccg attttcgtca gatattggct aattcgaccg
    16861 ttcacgccat aatcagcctt ttaacccgca aaaaggtaac gaataccatg gagatacgcg
    16921 tatttcgcca ggaagatttc gaagaggtca tcaccctttg ggagcgttgc gacttgctgc
    16981 gtccgtggaa cgatccggaa atggacatcg agcgtaagat gaaccatgac gtcagtttgt
    17041 ttttggtcgc tgaggtaaac ggtgaagtgg tcggaacggt gatgggcggt tatgacgggc
    17101 atcgcgggtc tgcttattat cttggcgtgc atccagagtt tcgtgggcgt gggattgcca
    17161 atgcgttgct taatcggctg gagaaaaagc tgattgctcg tggctgcccg aaaattcaga
    17221 tcaacgtgcc ggaagataac gacatggtgc tcggaatgta tgaacgcctg ggatatgaac
    17281 acgccgacgt gctgagtctg ggtaaacgtt tgattgaaga tgaagagtac tgagtttcat
    17341 cctgtccatt atgatgcgca cggtcgcctg cgtttaccct tgctcttctg gcttgtgcta
    17401 ttgcttcagg cgcgaacctg ggtgctgttt gtcattgccg gtgcgtcgcg cgagcagggt
    17461 actgcgctgc taaatctgtt ttatcccgat cacgataatt tctggctggg gctgattcct
    17521 ggcattccgg cggtgctggc gtttttgttg agcggtcggc gggctacgtt tcctcgcacc
    17581 tggcgtgtac tctattttct gttgctgttg gcgcaggtgg ttttactttg ctggcaaccg
    17641 tggctgtggc tgaacggtga atccgttagc ggtatcggtc tggcgctggt agtggcggat
    17701 attgtggcgt taatctggct gctgaccaat cgacgtttac gcgcttgttt taatgaagaa
    17761 aaagaataac ggcacttttt ggtgaatttg cactccaagc aacgttattg aataaccaaa
    17821 ggaagtgaca tgaaatcgct gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc
    17881 tgttccacga tgtcgtcagt taactggtcg gccgctaacc cgtggaactg gtttgggtcg
    17941 tccaccaaag tgagcgagca gggcgttggt gaattaacgg cgtccacacc actgcaagaa
    18001 caagccattg ccgatgcgct tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg
    18061 aacggcaacg tggtgcgctt ttttgaagtg atgaagggcg acaacgtggc gatggtgatt
    18121 aacggcgatc aaggcacgat cagtcgcatt gacgtgctgg atagcgatat tcctgctgac
    18181 accggtgtta aaatcggtac accgtttagc gacctttaca gcaaagcatt tggcaattgc
    18241 caaaaagctg atggtgatga taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat
    18301 attagctacc agttcagcgg ggaatggagt ggtcctgaag ggttaatgcc ttcggacgat
    18361 accctgaaaa actggaaagt cagtaaaatt atctggcggc gttaatttgc gtctgaacaa
    18421 accgccgccg cgaaaaaaca cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat
    18481 tattattttc aggaggaaca atgtctcagg ttcagagtgg cattttgcca gaacattgcc
    18541 gcgcggcgat ttggatcgaa gccaacgtga aaggggaagt tgacgccctg cgtgcggcca
    18601 gtaaaacatt tgccgacaaa ctggcaactt ttgaagcgaa attcccggac gcgcatcttg
    18661 gtgcggtggt tgcctttggt aacaacacct ggcgcgctct gagcggcggc gttggggcag
    18721 aagagctgaa agattttccg ggctacggta aaggccttgc gccgaccacc cagttcgatg
    18781 tgttgatcca cattctttct ctgcgtcacg acgtaaactt ctctgtcgcc caggcggcga
    18841 tggaagcctt tggtgactgc attgaagtga aagaagagat ccacggcttc cgttgggttg
    18901 aagagcgtga cctgagcggc tttgttgacg gtacggaaaa cccggcgggt gaagagacgc
    18961 gtcgcgaagt ggcggttatc aaagacggcg tggatgcggg cggcagctat gtgtttgtgc
    19021 agcgttggga acacaacctg aagcagctca accggatgag cgttcacgat caggagatga
    19081 tgatcgggcg caccaaagag gccaacgaag agatcgacgg cgacgaacgt ccggaaacct
    19141 ctcacctcac ccgcgttgat ctgaaagaag atggcaaagg gctgaagatt gttcgccaga
    19201 gcctgccgta cggcactgcc agtggcactc acggtctgta cttctgcgcc tactgcgcgc
    19261 gtctgcataa cattgagcag caactgctga gcatgtttgg cgataccgat ggtaagcgtg
    19321 atgcgatgtt gcgtttcacc aaaccggtaa ccggcggcta ttatttcgca ccgtcgctgg
    19381 acaagttgat ggcgctgtaa gcgtttctga ttcattgatt aaaggccagg cagtaacatt
    19441 actgactggc ctttttatta cttttgcttt aacgccgcat acacctgatc aactacccaa
    19501 ccatacgttg caatcggcag ctgaccgctt tcgaacatat tgggattctt ttgcggatcg
    19561 gcaaccggcg aatgtggctt gttgcttaac atcacaatcg ccatatgatt caccggatcg
    19621 ataacggtca ccgttccggt ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg
    19681 ccaaacgtcg gcgtcatggt ggcattacca ttcacgcgcc agccgaggcc aaaagtggca
    19741 tcttccttag agctggtggt gaacatcttc accgtttccg cactgaacag ctgcacatcg
    19801 ccatagcccc cgccgttcag catcgtttgc attaacaccg caatatcgcc ggtattggaa
    19861 aacaaacctg cgtgcccgga aacgccgccc atcgaataaa aggctttttc atcgtgcacc
    19921 tgaccccaga gagtggaggt gcggatattc gggaaatgga tcacgccatc gcgggtattg
    19981 ccgttcaatt ccgtggcggc aatttgctgc ggtttaaagc ctttcagcag cgggttaaat
    20041 accgtatgtg tcaggccgag cgggcgataa atcgactctt caacatagcg gtcaagcggc
    20101 tgaccggtga ccgactcgac gataaatcca agcagcatat aatcgacatc actgtaaata
    20161 tgcttgttgc cgggctgata ctccagcggc gtgcgcttga tcatctccag cgtctggcct
    20221 ttatcctggg aatataacgc gcccgcgacc gctttattcg gatattgtgg gtccgccggg
    20281 aaaccgccgc tgtgatgcag caggtcagaa atccgcagcg tgtttttgcc tttgatggtg
    20341 tcatttggac tatcggcaaa ccccggaata tatttcgcaa tccgatcgtc aggatgcagt
    20401 ttgccttcgc tcatcagctt ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag
    20461 gccaggtcat acagcgtccc ggtggtggct ttgacaggct gttccatcaa cacgctgcca
    20521 tcgtactttt tcgccgctcc ccaggctttg cgatacacaa tctgattatc tttaatgatc
    20581 agcaggttta cgccgggata accggcgtcc acttgctggc taatccagcg atccatctgg
    20641 ttaagccgtt cgacgttgaa ccctgctttc tctggcgaac tttctgtcag aacagggtat
    20701 tttgcggcgc tgacgctaca actaacagcc agcagagaaa gatagagcat tgtccgtttc
    20761 atgatatcag tccagattga cgttacggca gccaaagagc gtggtgaaaa taaacccgca
    20821 aacccatgcc accagaatcc cgccagcata aaccgccatt gccggaagga tgccttgtgc
    20881 ggaagtcatc agcggcagcg ccaccagacc agacggccca aaggcgctgt ttaagcccat
    20941 cggcagaccc caccaggcta tcaggccgat aaacaaaccg cccgctgcgc cgcctaaaca
    21001 ggcggtaata aacggtttca tgcggggcag ggtgacgccg taaatcaacg gttcaccaac
    21061 gcccagcaga ccgggaataa tcgccccgcg tacctgactg cgtaatgcac tgtgcggttg
    21121 cgcccgccag tagagtgcca gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat
    21181 gggaaataag ctgttgaatc cctggctgtc cattaacgcg aggtaaacag gaataaagcc
    21241 ctgatgcacg ccaaatacca cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa
    21301 cggattactg ttcaggtgca taaacagcca tgacatacct tcgaataacc agccacccag
    21361 cggcataatg atcaggtacg caagcgtagc ggtgatcagc agggtgatta acgaggtcag
    21421 cagcatgtcg agatcgtccg gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc
    21481 ccacgcggca atcagcacgc cgataatatt gccgcgcgga tcgatgggca gaccaaagaa
    21541 atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg ttataaccga gcaaaaagag
    21601 tgcggcaata attgcgccat tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc
    21661 caccagaatc accaggaaag tgaacaaacc tttgctgaac accttcataa aattcagcgc
    21721 gtcgggtagc gtgccctgag catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc
    21781 tatccccagc agcagaccgg cggcaataaa accggggatc agcggcgtga agatggtggc
    21841 gaatttggcg agaaattgtt gcacgccgga ggtttgtttg gcttttaact gacgtttgtt
    21901 ctgggcggcg atttctgcgg catcctgtac cggtgcctcg cccagcagct cgctcatcgc
    21961 ttttgcggca cgatgcgctt taccaggccc aaaaacgacc tgcacctggt cgctggtcaa
    22021 aatgacgccc ttcacgcctt ccagcgtttt gatattggga tcaaccagtg aactgtcatg
    22081 tacacccaga cgcaggcgcg tcatacagtt accacaactg gcgatatttc ccggtccgcc
    22141 gacacgggta aggatggtgt tcagaagttc actgctgatc tctttggcca tgggttattc
    22201 cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc aggcgttttt tagcttctgc
    22261 ggcatcgaga tttttcagca ccatcacaat ggccgtttta cagttgcgct cgcaggcaat
    22321 taacgccgct tccgcttgtt cggcattaca tccggtggcg tttttaacga tattgacctg
    22381 gcgcacatgc agtttttcgt tggtggcgac cacatcgacc atcaggttgc cgaacacttt
    22441 gccagattta atcatcagcc cggtggaaag catattgagc accagtttct gcgctgtgcc
    22501 tgctttcatt cgcgaagaac cagtgaccac ttcggcacct acaatcggtg taatggcaaa
    22561 ctcggctgtg gtggaaacgg cactccccgg attacaggaa attcccactg tgcggcagcc
    22621 gagctggcgt gcgtattcca gtccggcaat cacatacggc gtgcgaccgc tggcagcaat
    22681 gccaaccacc acatcctgtg ccgttaaatt aatatttttc agatcgttaa caccaccttc
    22741 ccggctatct tcagcgcctt ccaccgcgtg ctgaatggca tattcgcctc cagcaatcaa
    22801 accaaccacc agacccggtt tcacgccgta ggtgggcgga cattcgctgg catccagaat
    22861 ccccagacga ccggatgttc ccgcaccgag ataaatcaga cgaccgccgc cgctgacctg
    22921 ggcgtggata acatcgatcg ccgcggcgat atccggcagg acgcgctcaa cggcaagcgg
    22981 tacggtttta tcttcatcgt tgataatccg gcacatttcc agcgtcgata cgcggtcaat
    23041 ttcagccgag gcggtgttcg agccttcagt aatcatcttt tctagttgca tcggtggtga
    23101 ccttactatt tacgatcaaa taattgcaga tgtatggaat catatatccc gaattgacag
    23161 acttaaagga atcattgagt ctacactcgc agagtgtgaa gagcgtcata agaacttaag
    23221 cggaatttat gctgccagcc caggagagtg gaagatgttg tacctgacga aaataagcaa
    23281 tgcggggagt gaattcaccg aaaacgagca aaaaatcgct gactttctgc aggccaacgt
    23341 gagtgagctg caatccgttt cttcacggca gatggcgaaa cagctcggca ttagccagtc
    23401 gagcatagtg aaatttgccc aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc
    23461 gttaataggc gaatacagtg ccagccggga gaaaacgaat gccacggcgc tgcacctgca
    23521 cagttcgatt accagtgatg attcgctgga agtgattgcc cgcaaattga atcgcgaaaa
    23581 agagctggcg ctggagcaaa cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat
    23641 tgaggtcatc agtaaagcac cgtttatcca gataacgggc ctgggcggat cggcgctggt
    23701 ggggcgcgat ctgtcgttca aactgatgaa aattggctat cgcgtggcct gcgaagccga
    23761 cacgcacgta caagccaccg tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat
    23821 ctcttacagc ggcagtaaaa aagagattgt cttatgcgca gaagcggcac gaaagcaggg
    23881 ggcaacggtg attgccatta cttctctggc tgattctccg ctgcggcggc tggcgcattt
    23941 cacgctcgat accgtttccg gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac
    24001 ggcgcaaaat tctgttaccg atttactgtt tgttggtctt gtgcaactca atgatgtgga
    24061 gtcattgaaa atgattcagc gcagcagtga gctaacacaa cgcctgaaat gatgcataaa
    24121 gcagcgactg gattgagatt ttcctgaatt agtgagctga tccgcagcaa tattttgttt
    24181 atcctgtatt ttcagaggga atggagtgta acgctctgta ttaacaagga gagcattaaa
    24241 atgggtaaac tcacgggcaa gacagcactg attacgggcg cattgcaggg aattggcgaa
    24301 ggaattgcca gaacttttgc gcgtcatggc gcaaacctaa tcttgctgga tatctcccct
    24361 gagatcgaaa agctggcgga cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc
    24421 gccgatgtgc gtgacccggc gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa
    24481 gggcgcattg atatcctggt gaataacgca ggcgtttgtc gtctgggcag tttcctcgat
    24541 atgagcgatg acgatcgcga tttccatatt gacatcaata ttaaaggcgt atggaacgtc
    24601 acgaaggcgg tgctgccgga gatgattgcc cgcaaagatg gtcgcattgt gatgatgtct
    24661 tcggtcactg gcgatatggt ggccgatccg ggtgaaacgg cgtacgcctt aacgaaagcg
    24721 gcgattgttg gcctgacaaa atcgctggcg gtggagtatg cgcagtctgg tattcgcgtt
    24781 aacgccatat gccctggata cgttcgcaca ccaatggcgg aaagcattgc ccgccagtcg
    24841 aacccggaag atccagaatc ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc
    24901 ctcgccgatc cgttggaagt cggcgaactg gcggccttcc tcgcatcgga tgaatccagc
    24961 tatttaaccg gtacacagaa tgtgattgat ggcggcagca cactgccgga gacggtcagc
    25021 gtcggtatct gattcacctc tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttatt
    25081 cccttttcaa cttccaaatc accaaacggt atataaaacc gttactcctt tcacgtccgt
    25141 tataaatatg atggctatta gaaagtcatt aaatttataa gggtgcgcaa tggccgttaa
    25201 cttactgaaa aagaactcac tcgcgctggt cgcttctctg ctgctggcgg gccatgtaca
    25261 ggcaacggaa ctgctgaaca gttcttatga cgtctcccgc gagctgtttg ccgccctgaa
    25321 tccgccgttt gagcaacaat gggcaaaaga taacggcggc gacaaactga cgataaaaca
    25381 atctcatgcc gggtcatcaa aacaggcgct ggcgatttta cagggcttaa aagccgacgt
    25441 tgtcacttat aaccaggtga ccgacgtaca aatcctgcac gataaaggca agctgatccc
    25501 ggccgactgg cagtcgcgcc tgccgaataa tagctcgccg ttctactcca ccatgggctt
    25561 cctggtgcgt aagggtaacc cgaagaatat ccacgattgg aacgacctgg tgcgctccga
    25621 cgtgaagctg attttcccga acccgaaaac gtcgggtaac gcgcgttata cctatctggc
    25681 ggcatggggc gcagcggata aagctgacgg tggtgacaaa gccaaaaccg aacagtttat
    25741 gacccagttc ctgaaaaacg ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac
    25801 ttttgccgag cgtggcctgg gcgatgtgct gattagcttt gaatcggaag tgaacaacat
    25861 ccgtaaacag tatgaagcac agggctttga agtggtgatc ccgaaaacca atattctggc
    25921 ggaattcccg gtggcgtggg tcgataaaaa tgtgcaggcc aacggtacgg aaaaagccgc
    25981 aaaagcctac ctgaactggc tctacagccc acaggcgcag accatcatca ccgactatta
    26041 ctaccgtgtg aataacccgg aagtcatgga caaactgaaa aacaaattcc cgcagaccga
    26101 gctgttccgc gtggaagaca aatttggctc ctggccggaa gtgatgaaaa cccacttcac
    26161 cagcggcggc gagttagaca agctgttagc ggcggggcgt aattaatgtt tgcagtttca
    26221 tcaagacgcg tgctgccggg ctttacctta agcctcggca ccagtctgct gtttgtgtgc
    26281 ctgattttgc tgctgccgct ctccgcgctg gtgatgcagc tggctcagat gagctgggcg
    26341 cagtactggg aggtgatcac caacccgcag gtagtagcgg cctacaaagt aacgctgctg
    26401 tcagcgtttg tggcatcgat ttttaacggc gttttcggtc tgctgatggc gtggatccta
    26461 acccgctatc gcttcccagg ccgcacgctg cttgatgcgc tgatggattt accctttgcg
    26521 ctgccaacgg cagtcgccgg tttaacgcta gcctcgctct tttctgtaaa cggtttttac
    26581 ggtgaatggc tggcgaagtt tgatatcaaa gtcacctata cctggctggg gattgcggtg
    26641 gcgatggcct ttaccagcat tccgtttgtg gtacgtaccg tgcagccggt gctggaagag
    26701 ttaggcccgg aatatgaaga agcggcggaa acgcttggtg caacgcgctg gcagagtttc
    26761 tgcaaagtgg tgctgccgga gctttctccg gcgctggtgg cgggcgtggc gctgtcgttt
    26821 acccgtagtc ttggtgaatt tggcgcggtg atttttatcg ccggaaatat cgcgtggaag
    26881 acggaagtga cgtcgctgat gatttttgtg cgcttacagg agtttgatta cccggcagcg
    26941 agcgcgattg cttcggtgat cctcgcggca tctctgctgc tgctgttctc aattaacact
    27001 ctgcaaagtc gctttggtcg gcgtgtggta ggtcattaat ggcggaagtt acccaattga
    27061 agcgttatga cgcgcgcccg attaactggg gcaaatggtt tctgattggc atcgggatgc
    27121 tggtatcggc gttcatcctg ctggtgccga tgatttatat cttcgtacag gcgttcagca
    27181 aagggctgat gccggtttta cagaatctgg ccgatccgga catgctacac gccatctggc
    27241 tgacggtgat gatcgcgctg attgccgtac cggtaaacct ggtgttcggc attctgctgg
    27301 cctggctggt gacgcgcttt aacttccctg gacgccagtt actgctgacg ctactggaca
    27361 ttccgtttgc cgtatcgccg gtggttgccg gtctggtgta tctgctgttc tacggctcta
    27421 acggcccgct gggcggttgg ctggatgaac ataatctgca aattatgttc tcctggccgg
    27481 gaatggtgct ggtcaccatc ttcgtgacgt gtccgtttgt ggtgcgcgaa ctggtgccgg
    27541 tgatgttaag ccagggcagt caggaagacg aggcggcgat tttgcttggc gcgtctggct
    27601 ggcagatgtt ccgtcgtgtc acattgccga acatccgctg ggcgctgctt tatggcgtag
    27661 tgctgaccaa cgcccgcgcg attggcgagt ttggcgcggt gtcggtggtt tccggctcga
    27721 ttcgcggcga aacactttcg ctgccgttac agattgaatt gctggagcag gactacaaca
    27781 ccgtcggctc ctttaccgct gcggcactgt taacgctgat ggcgattatc accctgtttt
    27841 taaaaagtat gttgcagtgg cgcctggaga atcaggaaaa acgcgcacag caggaggaac
    27901 atcatgagca ttgagattgc caatattaag aagtcgtttg gtcgcaccca ggtgctgaac
    27961 gatatctcac tggatattcc ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc
    28021 gggaaaacca cgctgctgcg cattatcgcc gggctggagc atcaaaccag cgggcatatt
    28081 cgcttccacg gcaccgacgt cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg
    28141 ttccagcatt acgcgctgtt ccgccatatg acggtattcg acaatatcgc ttttggcctg
    28201 acggtgctgc cgcgtcgcga gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa
    28261 ttgctggaaa tggtccagct tgcgcatctg gcggatcgtt atccggcgca gctttccggc
    28321 ggccagaaac agcgtgtggc gctggcgcgc gcgctggctg tagaaccgca aattctgctg
    28381 cttgatgaac cgtttggcgc gctggatgcg caggtgcgta aagagctgcg tcgctggctg
    28441 cgtcaactgc atgaagagct gaaattcacc agcgtgtttg tgacccacga ccaggaagag
    28501 gcgaccgaag tagccgatcg tgtagtggtg atgagtcagg ggaatattga acaggctgat
    28561 gcgccgaatc aggtatggcg cgaaccagcg acccgttttg tactcgaatt tatgggcgaa
    28621 gtgaaccgcc tgcagggaac cattcgcggc gggcagttcc atgttggcgc acatcgctgg
    28681 ccgctgggct acacacctgc ttatcagggg ccggtggatc tcttcctgcg cccgtgggaa
    28741 gtggatatca gccgccgtac cagtctcgat tcgccgctgc cggtacaggt actggaagcc
    28801 agcccgaaag gtcactacac ccaattagtg gtgcagccgc tggggtggta caacgaaccg
    28861 ctgacggtcg tgatgcatgg cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt
    28921 ctgcaacatg cgcggctgta taacggcgac gagcgtatcg aaacccgcga tgaggaactt
    28981 gctctcgcac aaagcgcctg ataggttgag tgaatgttaa acgcccggag gcgcttcccg
    29041 cgatccgggc tttttaatgg caaggtttgt aacctgtaga cctgataaga cgcgcaagcg
    29101 tcgcatcagg caacaccacg tatggataga gatcgtgagt acattagaac aaacaatagg
    29161 caatacgcct ctggtgaagt tgcagcgaat ggggccggat aacggcagtg aagtgtggtt
    29221 aaaactggaa ggcaataacc cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat
    29281 cgtcgaggcg gaaaagcgcg gggaaattaa accgggtgat gtcttaatcg aagccaccag
    29341 tggtaacacc ggcattgcgc tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt
    29401 gctgatgccc gacaacatga gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga
    29461 actgattctt gtcaccaaag agcagggcat ggaaggtgcg cgcgatctgg cgctggagat
    29521 ggcgaatcgt ggcgaaggaa agctgctcga tcagtttaat aatcccgata acccttacgc
    29581 ccattacacc actaccgggc cggaaatctg gcagcaaacc ggcgggcgca ttacccattt
    29641 tgtctccagc atgggaacaa ccggcactat caccggcgtt tcacgcttta tgcgcgaaca
    29701 atccaaacca gtaacgattg ttggcctgca gccggaagaa ggcagcagta ttcccggcat
    29761 tcgccgctgg cctgcggaat atctgccggg gattttcaac gcttctctgg tggatgaggt
    29821 gctggatatt catcagcgcg atgcagaaaa caccatgcgc gaactggcag tgcgggaagg
    29881 aatattctgt ggcgtcagct ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc
    29941 taaccctggt gcggtggtgg tagcgatcat ctgcgatcgt ggcgatcgct acctttccac
    30001 cggggtgttt ggggaagagc attttagcca gggggcgggg atttaagatt attgcgcagg
    30061 caccgtttta tccgtctgcg caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct
    30121 cttaccgggg atcttcacaa tatacggctt cccggtaatc gatgacgacg aagcaacctt
    30181 atcaataaac tgctcggcag tgtcaatgcg gttacgggta ttgcccagct tcagacgcag
    30241 atgagaaacc gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt
    30301 tttctgtgct aatccctcca gcatggcgtt gatgcgggct tcttcatggg cagttaactt
    30361 cgcgtaagcg gggagtgtca ttagtagtgt aatgaccaga caaatgattt tcttcattga
    30421 attaccttat ccttgttttg tgatagccga tttgactgcc agaatcaggt aaagtttcgg
    30481 ttggaatacg atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt
    30541 ttaacgataa gagcagggcg ctgcaggcgg atatcgtctc cgtgcagtcg caggtggttt
    30601 acggcagcgt gggcaacagc attgccgtgc ctgctatcaa acagaacggt ctgaatgttt
    30661 ttgccgtgcc gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg
    30721 cgattccgga tgaatggttt agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc
    30781 gccagctgcg cgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg
    30841 ccgagtggct gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg
    30901 tgattggcga tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac
    30961 aatatttact gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga
    31021 ccggtaaaaa ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag
    31081 acacattaaa atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc
    31141 aggtggtggt ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg
    31201 acctgaaagg gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga
    31261 aggcgttaaa cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca
    31321 cccagcagca tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg
    31381 cgccgatggg cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg
    31441 tttcacccac ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg
    31501 agataacaac cggagtcagg gtagacttgg ctttctcttc cagtagcggc agatcaaatt
    31561 caatgacagt atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt
    31621 cgcctttcag ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag
    31681 attcgataga gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg
    31741 cgaccatttt gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa
    31801 aaacgacatc cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct
    31861 caatagttcc ggtatccttc ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac
    31921 ccatgatctt ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt
    31981 tcttcaatga acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc
    32041 tgctctgcta acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc
    32101 gggatagaaa tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta
    32161 gcacgttcat cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa
    32221 gcatcaataa cttgcttgat caagttcagc acggacggtg acattggttg gtaaaggtgt
    32281 gaaatcatat cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg
    32341 atactaaaga aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt
    32401 tccaccatta cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc
    32461 tgtttgtaga tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc
    32521 gggaacataa tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg
    32581 cgcaggatct ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc
    32641 tcttctttcg ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga
    32701 acgataaccg cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct
    32761 tcttcagtgg gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg
    32821 ccttcagcgc cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac
    32881 acttctacct ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct
    32941 gctttttcag aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt
    33001 ggattgacgt aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc
    33061 tgagaggtga cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata
    33121 gaggtgtggg aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc
    33181 agctgtgcgg tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg
    33241 gcgctcaggt cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca
    33301 cgtacgtcag ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc
    33361 tgaccttcga taacttcatg agcagctgcg tcagctgtca tgtgcttatc tttaatcagg
    33421 gctatgattt cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg
    33481 gcttcttttt cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct
    33541 gatgccttgg cacgaccgct cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa
    33601 atttttttcc ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg
    33661 atacccgggg atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt
    33721 aaaagaaccc gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt
    33781 tctgctcgtc ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag
    33841 tctgcagttt aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt
    33901 cagaagtgaa gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt
    33961 tcggagcggt aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag
    34021 tgttgtggaa ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt
    34081 ttttaacaga cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg
    34141 ctgacgtttc gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata
    34201 aaatcgattc agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt
    34261 ctggttaaat accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc
    34321 aggccacaaa aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta
    34381 ctgttgcagt tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc
    34441 acccgatgac ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag
    34501 tttcaacgcc gcagcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc
    34561 catcagacga cgtgcggtag aaatcgcttc ttcattggtg atgccaatga ctttatcgac
    34621 cagcttgaga tcgaggttag ctgggataaa accagcgcca ataccctgaa ttttatgcgg
    34681 gccaggttta atctcttcac cagccagtgc ctgggcgata actggagaat cggttggctc
    34741 aacggcgaca gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc
    34801 agtcagcgta ccgccagtcc caacgccagc aataaataca tcaacctgac cgtcggtatc
    34861 ttcccagatc tccggaccgg tggttttttc gtgaatttca gggtttgccg gattgctgaa
    34921 ttgttgcagc agcaggtatt tctctggatt gctggcgaca atttcttctg ctttttggat
    34981 tgcgcctttc atgcctttag caccttccgt cagcaccagg tttgcaccta acgctttcag
    35041 cagcttgcgg cgttcaatac tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg
    35101 agcggcagct acataggcca gtgcaatccc ggtattaccg ctggtcggtt caaccagttc
    35161 aacgcctggt ttcagcacgc cgcgcttttc ggcatcccaa atcatgttgg caccgatacg
    35221 gcacttaacg ctgaagctgg ggttacgaga ttccaccttc gccagaatgc gtccgttacc
    35281 gatgcgattc aggcgaacca gcggcgtgtg accgatagtc agcgagttat cttcaaaaat
    35341 cttactcatg gcctgtcctt aactgtatga aattgggata caacaggtag catacccgct
    35401 cagagaatat gcggaagtaa ggatttagca tatctatata cagaagggaa ataatgacat
    35461 gcaagatgga ataaggggcg gcataagcca cccctgtttc acacaaacgg tttacaaaat
    35521 aaccggtaga ttgttaccgc cacatcgcgt gtttatcgcg atagcaatcg acccacatcg
    35581 ccgtcgcgcc acaaacggca acgggcatga tgaacagatt aagcagcggg atcatcgtaa
    35641 acaggctggt taaagcacca aactgcatat tggtgatttt gcgtgtgcgc agggctgtgc
    35701 gcatctcttt aaacggcact ttgtggttat cgaaggggta atcgcaatac tggatggcta
    35761 acatccaggc gctaaacagg aaccacagta ccggcgcgac ggtttgccca ataccaggga
    35821 ttaagtaaag aattagcaat acaattgcgc gcggcagata ccaggcaaat ttttgccatt
    35881 cgcgtttcat gattcgcggc acatctttca tgataccgaa aatcccggta tctggcggtg
    35941 tagcgccagt caatcgtgct tccagttgtt cagccaataa accgttaaac ggagcggcaa
    36001 tccagttagc aatcgtggag aagaaatagc caaacactaa cagcacagag atgacagcca
    36061 gaggccacaa cagataactc agccattgta gccagtccgg aacgtaactc atgagagtcg
    36121 ggatccagac atcgagctgt gtaaagagcc accagaatgc gccccccatc aacaaaatat
    36181 tgaccagcag cggtaaaata acgaaacgcc gaatcccagg ttgcgagacg agcttccagc
    36241 cttgcgcaaa atagtaaaaa ccgctgcgtg gggcagatgt gaatgatgaa accataatca
    36301 ggatgagctc cttttgacca atcccaggaa aattctgcgt attttaccgg gtaattgcgc
    36361 aatggacagt taggatatgt tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt
    36421 gctgtgaaag ttaatagtgc acttgcactt gaggtaatcg gcaaatactc ttagtgagta
    36481 aatgtttgcc gtggtggcaa ggtgttagaa caacagagaa tataatgatg caggatttgc
    36541 gtctgatatt aatcattgtt ggcgcgatcg ccataatcgc tttactggta catggtttct
    36601 ggaccagccg taaagaacga tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt
    36661 caaaacgtga cgacgattct tatgacgagg atgtcgaaga tgatgagggc gttggtgagg
    36721 ttcgtgttca ccgcgtgaat catgccccgg ctaacgctca ggagcatgag gctgctcgtc
    36781 cgtcgccgca acaccagtac caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc
    36841 agcagccgcc tgaagcgcag gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc
    36901 agcagcctgc ctatcagccg cagcctgaac agccgttgca gcagccagtt tcgccacagg
    36961 tcgcgccagc gccgcagcct gtgcattcag caccgcaacc ggcacaacag gctttccagc
    37021 ctgcagaacc cgtagcggca ccacagcctg agcctgtagc ggaaccggct ccagttatgg
    37081 ataaaccgaa gcgcaaagaa gcggtgatta tcatgaatgt cgcggcgcat cacggtagcg
    37141 agctaaacgg tgaactgctt cttaacagca ttcaacaagc gggcttcatt tttggcgata
    37201 tgaatattta ccatcgtcat cttagcccgg atggcagcgg cccggcgtta ttcagcctgg
    37261 cgaatatggt gaaaccggga acctttgatc ctgaaatgaa ggatttcact actccgggtg
    37321 tcaccatctt tatgcaggta ccgtcttacg gtgacgagct gcagaacttc aagctgatgc
    37381 tgcaatctgc gcagcatatt gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc
    37441 gtatgatgac tccgcagaaa ttgcgcgagt accaggacat catccgcgaa gtcaaagacg
    37501 ccaacgcctg atacacttaa ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt
    37561 ttttagcatt gatggtgcga tatggaatca atcgaacaac aactgacaga actgcgaacg
    37621 acgcttcgcc atcatgaata tctttatcat gtgatggatg cgccggaaat tcccgacgct
    37681 gaatacgaca ggctgatgcg cgaactgcgc gagctggaaa ccaaacatcc agaactgatt
    37741 acgcctgatt cgcctaccca acgtgtaggc gctgcgccgc tggcggcttt cagccagata
    37801 cgccatgaag taccaatgct gtcactggat aacgtttttg atgaagaaag ctttcttgct
    37861 ttcaacaaac gtgtgcagga ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag
    37921 ctgaagctgg atggccttgc cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc
    37981 gcgacccgtg gcgatggcac caccggggaa gatatcacgt ctaatgtgcg tactattcgc
    38041 gctattccgc tgaagctgca cggagagaat atcccggcgc gtctggaagt gcgtggtgaa
    38101 gtgttcctgc cgcaggcggg gttcgaaaag attaacgaag atgcgcgacg cacgggcggg
    38161 aaagtttttg ctaacccacg taatgcggca gctggttcac tgcgtcagct tgatccgcgt
    38221 attacagcga agcgaccgct cacttttttc tgctatggcg ttggtgttct ggaaggtggc
    38281 gagctgccgg atactcatct tggccgttta ctgcaattta aaaagtgggg gttgccggtc
    38341 agcgatcggg taacgctttg tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg
    38401 gaagaagacc gcccgacgct gggctttgat atcgacggcg tggtgattaa ggtcaactca
    38461 ctggaacagc aggagcagct tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt
    38521 aaattcccgg cgcaggagca gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt
    38581 actggcgcga ttacgcctgt tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg
    38641 agtaacgcaa ccttacacaa tgcggatgaa atcgaacgtc ttggtttacg cattggcgat
    38701 aaagtggtga ttcgccgcgc tggcgacgtg atcccgcagg tggttaacgt cgtgctttct
    38761 gaacgcccgg aagatacccg tgaggttgta ttcccgacgc attgtccggt atgtggttct
    38821 gacgttgagc gtgtggaagg tgaagcggtt gcccgctgta ccggtggcct gatttgcgga
    38881 gcgcagcgta aagagtcgct gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga
    38941 atgggcgaca aaatcatcga tcagctggtt gaaaaagaat atgtccatac tccggcagat
    39001 ctgttcaaac tcaccgcagg caaactgacc ggactggagc gtatggggcc aaaatcggca
    39061 caaaacgtgg ttaacgcgct agaaaaagcg aaagaaacca cctttgctcg cttcctctat
    39121 gcacttggca tccgtgaagt cggcgaggcc accgcagcag gtctggcggc atatttcggc
    39181 acgctggaag cgctggaagc cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc
    39241 attgtcgttg catcccacgt tcacaacttc tttgccgaag aaagcaaccg caatgtcatc
    39301 agcgagctgt tggcggaagg tgttcactgg cctgcgccga tcgttatcaa cgcggaagag
    39361 attgacagcc cgtttgctgg taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg
    39421 cgtgatgacg ctaaagctcg actggttgaa ctgggcgcga aagtcgcggg cagcgtgtcg
    39481 aagaaaaccg atctggtgat tgccggagaa gcggcaggtt ctaaactggc gaaagcgcag
    39541 gaactgggca ttgaagtcat cgacgaagcg gaaatgctgc gtttgctggg tagctgagat
    39601 ggaaaaagag cagctgattg aaatagccaa tacgataatg ccgtttggca aatataaagg
    39661 gcgtcgctta atcgacctgc cggaggagta tctgctgtgg tttgcccgca aagatgaatt
    39721 cccggcaggg aagctcggtg agctaatgca aatcacgctg ctgattaaaa ctgaggggct
    39781 gacgcaactg gtccagcccc tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg
    39841 ctgtgcctgt aactgttcgg tctggcgttt gtatcgacgc gccagcaccg cacagaccat
    39901 caattggatc tgatggaaaa tcattagggg cagcaccatc ataccgatca ccgatgtggg
    39961 gaacagaatg tttgccatcg ggatgccatt tgccagactc tttttcgaac cacaaaagac
    40021 gatagtaatt tcatctgcct tattgaagcc cagtcggcgt gccataaaga cgttaactac
    40081 aatcacgata gccagaagaa cgcagctgac caccacgata aacagcaatg atccccagcc
    40141 aactttatgc cagataccat taacgacggc ttcgctgaac gctgtataaa ccaccaacag
    40201 aatggacgtc tggtcagttt tcgcaatcca ttttttattg cgcgacaccc agtcaccaat
    40261 ccacggccgg gaaagatgcc ccaacacaaa cggcagcagc agttgcagca taattttacc
    40321 gacctgctca aggctgcccc ctgcaccgtg aacattcatc accagaccaa ccagcaatgg
    40381 tgaaaggaaa atccccagca ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt
    40441 accgcccgcc attgacgtga aggcgattgc agactgcacg gtagccggga gaatgcacaa
    40501 gtagagaaaa ccggagtaga gcatcgggtc gacatttacc ggtttccacc aggcaaacag
    40561 tacacccaga atcggaaaca gcacgaaggt gctgcaaatt acccacaaat gcagtcgcca
    40621 gtgaccaccg ccagcaataa tcgcctcacg cgacaacttc gcgccgtgca taaagaacag
    40681 cagggcaatc gctgcggtgg tcagattttc aaagaagggg acgaaatcgc ctctggccgg
    40741 aaagaaagag gccagcaaca ccaccgtgat cagggttaag gtgaaaggat cgaggatacg
    40801 aaaaagtttc ataaagactc cagatgatcg atggttctat tgtgcttctt ccagtttgag
    40861 aaataaaatt gatttattgc atccattcat gaagaaaaca gatgaattat tctttaaaac
    40921 aattaaaggt tttcgtcaca gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta
    40981 tcggcctgag ccagtcggca gtgagtcaca gtgtgaagga actggaaaat catactggtg
    41041 ttcgcctgct ggacagaacc acgcgtgaag tggtgcttac agatgcaggg cagcagctgg
    41101 ctttgcgtct tgagcgactg ctggatgaac ttaacagcac gttgcgcgat accgggcgta
    41161 tggggcaaca actgagcgga aaagttcggg tcgctgccag ccagaccatt tccgcgcatc
    41221 ttattccgca atgcattgct gaaagccatc gccgctatcc agatattcag tttgtcctgc
    41281 acgatcgtcc gcagcagtgg gtgatggaaa gtattcgcca gggagatgtc gactttggca
    41341 tcgtcatcga tccaggccca gtgggcgatc tgcaatttga agcgattctt tccgagcctt
    41401 tctttctgct ttgccatcgc gatagcgctt tggccgtgga agattatgta ccctggcagg
    41461 cattgcaggg agcgaaattg gtgttgcagg attacgcgtc aggcagccga ccgctgattg
    41521 acgcagcgct ggcgcgcaat ggtattcagg cgaatattgt gcaggagatt ggacatccgg
    41581 cgacgctgtt cccgatggta gccgcaggca tcggcatcag tattcttccc gcactggcgc
    41641 taccattgcc tgaaggtagc ccactggtgg taaagcgcat tacgccggtt gttgaacgcc
    41701 agttgatgct ggtgcgcagg aagaaccgtt cactttccac cgccgctgaa gcgttatggg
    41761 acgtggtacg agaccagggc aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc
    41821 agatatagat atcgatatga gtcgtttttt caacaggatt tagaagggat acattcagag
    41881 acaaacactg ctctttctcc tgcgcctttt ctgcctgctg tttctgcaac tgcgccagtt
    41941 gtgtttccag tagggtgatt tgctgttgaa tcaattcggc ctgtttttgc ttgtcttcag
    42001 cactgccgct agtgtctaca atctctttaa tttgttgagt gagtttgatg atttgctgac
    42061 ttatccgcgc gatttgtgaa gcaacatcgc taccttgtga tgccctgtta ctactctgtg
    42121 tttgtatgga aggaattgaa gttgaagaga tagtggccat tgttcagtcc tcgtcactgt
    42181 acgaggtaat atcggtagaa gaacaaggaa cttgagaaga aaaatcaatc ttacaaatcg
    42241 cagatagcaa aaaagcgcct ttagggcgct tttttacatt ggtgggtcgt gcaggatgac
    42301 tcgcttcgct cgcccttcgg gccgtcgccg cgagcggctc cgttgtttcg ctgcgctcaa
    42361 ctcaaacctg ctgcaggttc gaatctttga gtatcgcaga tagcaaaaaa gcgcctttag
    42421 ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    42481 tcaactgctc taccaactga gctaacgacc cgaagtggtg ggtgatgacg ggatcgaacc
    42541 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cacttcggta
    42601 cttcacattt ttacaagaaa tttagctggc gagaaagtgg tgggtgatga cgggatcgaa
    42661 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccacttctc
    42721 aatttcttgc tacacggcgg agactacata aagtagttgg tgggtgatga cgggatcgaa
    42781 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccccgctgt
    42841 gtggagtcgc attataggga gagttcaaaa tgagtcaacg cattttctaa agaaattgtt
    42901 cgttcgtcgt aaatttaagc aagatgatcg caaaacagac cgtgttgcgc aatttgtcaa
    42961 cgaaaacaat aatgcgtaag gtagaaaccc gaactacatt gaggaatcag gcgggagtga
    43021 tagaatatcg cccactcaat ttttccagga tttgccggtt gtcggcatct ttctaaacgt
    43081 aaggccattt catgaaaatc aaaactcgct tcgcgccaag cccaacaggc tatctgcacg
    43141 ttggcggcgc gcgtactgct ctttactcct ggctttttgc acgtaaccac ggcggtgagt
    43201 tcgtgctgcg tattgaagac accgatcttg agcgttccac gccggaagct atcgaagcca
    43261 ttatggatgg catgaactgg ctgagcctgg agtgggatga aggtccgtac taccagacca
    43321 aacgttttga tcgctacaat gcggtgatcg atcagatgct ggaagagggc actgcttata
    43381 aatgctattg ctctaaagag cgcctggaag cgctgcgcga agagcaaatg gcgaaaggtg
    43441 agaagccgcg ttatgacggt cgctgccgcc acagccatga gcatcatgct gatgatgaac
    43501 cgtgtgtcgt gcgttttgct aatccgcagg aaggttctgt tgtttttgac gatcagatcc
    43561 gtggtccgat cgagttcagc aaccaggagc tggatgatct gattatccgc cgtaccgatg
    43621 gttccccaac ctataacttc tgtgtggttg tcgatgactg ggatatggaa atcacccacg
    43681 ttatccgtgg cgaagaccat atcaacaaca cgccgcgcca gatcaacatt cttaaggccc
    43741 tgaaagcgcc ggtgccggtt tacgcgcacg tttctatgat caatggcgat gacggtaaaa
    43801 aactgtccaa acgtcacggg gcagtcagcg taatgcagta tcgtgatgac ggttatttgc
    43861 cagaagcact gctgaactat ctggtgcgtc tgggctggtc ccacggcgat caggaaatct
    43921 tcactcgtga agagatgatc aaatacttca ctttgaatgc cgtcagcaaa tctgccagtg
    43981 cgttcaacac cgacaagctg ctgtggctga accatcacta cattaacgcg ctgccgccgg
    44041 agtatgttgc tactcactta cagtggcaca ttgagcagga aaatatcgat acccgtaacg
    44101 gcccgcagct ggctgatctg gtgaaactgc tgggcgaacg ctgcaagacg ctgaaagaga
    44161 tggcacagag ctgccgttat ttctacgaag attttgctga gttcgatgcc gatgcggcga
    44221 aaaaacatct gcgtccggta gcgcgtcagc cgctggaagt ggttcgtgac aaactggccg
    44281 cgattactga ctggaccgct gaaaacgttc atcacgctat tcaggcgacg gcggatgagc
    44341 tggaagtggg tatgggtaaa gttggtatgc cgctgcgtgt cgccgtaacc ggtgcggggc
    44401 agtctccagc actggatgtt accgttcacg caattggtaa gacccgcagt atcgagcgta
    44461 tcaacaaagc gctggatttt attgctgaac gcgaaaatca gcagtaatta gcgtgtaaaa
    44521 agataaacgg caggagataa tatcctgccg ttttttattt atgctgattc gtcaatatcg
    44581 agacgggcga ggaaattgcg aattccttca cgtgacaaaa attgcgcgac tttttcctgc
    44641 tcaatgtctg tcatattatc tatcgcgttg atgatttgtc gatacgccgg ggcgcgtgct
    44701 ccaggagcgt attcagcaat tctctcttca gcttccatca acattggtgt gttatggaag
    44761 gctggcgtgt tctgaataaa ctcgcaaact tgcgtatcga ccagaataag tctggcacga
    44821 ccgcctttca cgccgggaaa tttttccgtt ttccagcctt tttctctgat ccaacggtta
    44881 acagtttgtt tagccacacc gaggcattca gccagttctt cggtggtcat tttgttgcgt
    44941 aatcttttca tattatttgc tatcgcgaat ccctaagcgt tgtaacaatc cgataatccc
    45001 ttcccgcaga agcaaggatg taaactgttt ttgttcaact ggcgtcatct ctttcgccag
    45061 tgtcaccagc aatacttcaa gcggcgcatc accagaaagg gcaggcgctt ctccctgacc
    45121 ttctggacgt tcagcattgc gaatatattc acgaacctgt tcattgacgt gaaccagtcg
    45181 ggctttgcca ccctggacgc caggttttgg tgacgttgtc cagccttcct tgcgtaccca
    45241 tttattaatg gtctggcggc tatagccggt cagtctggca agttcatctg gtgtcatcct
    45301 ctccttgaac ataacaattc cctgaatgta tgtgggtcta ttagtagttc attttataac
    45361 accactttac aggaaaccgt gacatgaata actactgaat gcgagcggtt ttccaaaatt
    45421 gttcatttgc ctgatttttc agcgattgaa actacacgtt gcaatttgcc gttgacacat
    45481 tcgggcggaa ttcatatgat gccgcccgtc aactcgacaa gctttacgtg acggggctat
    45541 agctcagctg ggagagcgct tgcatggcat gcaagaggtc agcggttcga tcccgcttag
    45601 ctccaccaaa ttttgcaccc agcaaacttg gtacgtaaac gcatcgtggg gctatagctc
    45661 agctgggaga gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg cttagctcca
    45721 ccaaatttcc aaccctcgct gcaaagcggg ggttttttgt ctctgctttt tgccgctttt
    45781 gtaatacagt ctacgtccgg gttagtgccg cctggtgaaa gcatcattgg atgaaaaatc
    45841 ggcaacaggc tggccccctg tttgcttcgc gatgcgaata aacttattat ttgtgtgcct
    45901 gaaaaccccg atcagtgaga gtagtgtact catgtttgtg gagcataacc tgataaaaaa
    45961 tatcaagata ttcacactag cgtttacgct caccgtggta cttattcagc tatcccgttt
    46021 tatttcgcca cttgccatta tccattccag ttatatcttt ctggcgtgga tgccactgtg
    46081 cgtaatgctg tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg ttttatgcgg
    46141 gatgttttgc accaatctgt ggaactttca tctctctttt ttacagactg cggtcatgct
    46201 cggtagccag acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc agctggggac
    46261 gcgttggcgt tatggattga ccagccgata tgtctggcaa cgtctgttct ggcttggttt
    46321 ggtgacgccg atcggcatca aatgcagcat gtatcttgtg gggagcttct ttgatttccc
    46381 gctaaagata tccacctttt tcggcgatgc ggatgccatt ttcacggtcg ttgatttgct
    46441 aagccttttc accgctgtgc tgatttacaa catgcttttc tactatctca ctcgcatgat
    46501 tgtaagtccc cactttgcgc agatattgtg gcgcagggat atcgctccgt cgttgggcaa
    46561 agagaaacgc gcatttacct taagctggct ggcagcgtta agcgtgctgc tacttctgtt
    46621 gtgcacacct tatgaaaacg actttattgc cggttacttg gtacccgttt tcttcatcat
    46681 ctttaccctc ggggtcggta agcttcgcta tccgttttta aatctcacct gggctgtttc
    46741 aacgctttgc ctgctgaatt acaaccagaa ctttttgcaa ggggtggaaa ccgaatattc
    46801 gctggcattt attcttgcgg tgctgatttc ctttagcgtt tgcctgctct atatggtgcg
    46861 catttatcat cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc tgacagatcc
    46921 gttaacgctc ctacccaact ttcgtgcgtt ggaacaagcg ccggagcaag aggcgggcaa
    46981 gagtttttgc tgcctgcgca ttgataatct tgagtttatg agtcgtcatt acggcttaat
    47041 gatgcgcgtt cactgtatcc gctcaatttg ccgtacgctg ctgccgttga tgcaggaaaa
    47101 cgaaaagttg tatcaattgc cgggtagtga actgctgtta gtgctgagcg ggccggaaac
    47161 ggaagggcga ctccagcata tgattaacat cctgaatagt cggcaaattc actggaacaa
    47221 taccgggctg gatatgggct atggtgctgc ctgggggcgt tttgatggaa atcaggaaac
    47281 cctgcaaccc ttgttggggc agttaagctg gctggcggag caatcctgcg cacatcatca
    47341 tgtgctggcg ctggatagca gagaggagat ggtttccggg cagaccacta aacaggtgct
    47401 attgctgaat accattcgca cggcgttaga tcagggtgat ttgctgctct acgcccagcc
    47461 aattcgcaac aaagagggtg aaggttatga tgagatcctc gcgcgactga aatatgacgg
    47521 cggcattatg accccggata agtttctgcc ccttattgct cagtttaacc ttagcgcgcg
    47581 ttttgatttg caagtgctgg aatccttgtt gaagtggctg gcaacacacc cttgcgacaa
    47641 aaaaggaccg cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa agaatattgc
    47701 cgggcggatt attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg tcattcttga
    47761 gatcaccgag gagcaggcgt tttctaacgc agaaagcagc atgtacaaca tcgagcagct
    47821 gcataagttt ggtttccgga ttgcgattga tgactttggc accggatatg ccaactacga
    47881 acggttaaag cgtttgcagg ctgatatcat caaaattgat ggcgtctttg tgaaagatat
    47941 tgtcacgaat acgctggatg cgatgattgt gagatcaatt accgatctgg cgaaagcgaa
    48001 gtcattgagt gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc tattgcataa
    48061 gctcggggtg caatatctgc aagggtattt gattggtcgc ccgcagccat tagctgatta
    48121 acaggcgtaa aaaagccggg gaatgatccc cggctttttt atgtatggtt ttacagcacc
    48181 agtgctgcga ttgacgcaga cagcacactc accaaggtag agccgtaaac cagtttcaga
    48241 ccgaagcgag aaaccacgtt accttgctct tcattcaggc ctttaactgc acctgcgata
    48301 atcccgattg aagagaagtt agcgaaggaa accaggaaca cagagatgat gccttcagca
    48361 cgcggagaga gcgtggaagc aattttctgc agatccatca tcgcaacgaa ctcgttggaa
    48421 accagtttgg tcgccatgat actgcccact tgcagtgctt cactggaagg aacacccatc
    48481 acccatgcaa tcggatagaa gatgtagccc aggatgccct ggaaggagat gctgtagcca
    48541 aaccagccag taacggtggc aaacagtgcg ttcagcgcgg cgatcagggc gataaagccg
    48601 atcagcatcg cggcaacgat aatggcaact ttgaaacctg ccagaatgta ttcacccagc
    48661 atttcgaaga agctctgacc ttcgtgcagg ttggacatct ggatgttttc ttcactggca
    48721 tcaacacggt aaggattgat cagcgacagc acgataaagg tgctgaacat gttcagtacc
    48781 agcgcagcaa cgacgtattt cggttccagc atggtcatgt atgcaccaac gatggacatc
    48841 gacacggtgg acattgccgt ggcagccatg gtgtacatac gattacggga gattttgccg
    48901 aggatatctt tataggcaat aaagttttca gactgaccca gaatcaggga gctgacggcg
    48961 ttaaaggatt ccagtttgcc cataccgttg actttggaga gcaggaaacc aattgcgcgg
    49021 ataataaccg gcaacacgcg aatgtgctgg agaataccga tcagtgcaga gataaagacg
    49081 attgggcaca gcactttcag gaagaagaat gccaggcctt gatcattcat gctaccaaag
    49141 acgaagttag tcccttcgtt ggcaaatccg agcagttttt cgaacatttc ggagaagcct
    49201 ttcacgaagc ctaaaccaac gtcggagttc aggaagaacc acgccagtaa cacttcgata
    49261 acaagcagtt gaataacata acggatacga atttttttgc ggtcgctgct taccagcagt
    49321 gcgagaatcg caacaacggc aagtgccagt acaaaatgaa ggacgcggtc catatttgct
    49381 ccaaatatga ggcaggttaa atttccgtgc acattctatg taacatggtt aaggaaaacg
    49441 agatctgaca cacactataa tgacgtcctg tgacaagctt caaaaatagt gatctgtcat
    49501 acatttctgc tatgttgtgt atggaagctg aaagttatgt aaatgtgcta acattaaaca
    49561 taaccataac attcaagtca tcaatctttt cgtagtcata atcctggtct atcaaagaaa
    49621 tcaccacaac ccattcaaat gaatttgata atcattctcg tttggcatag catgaaacat
    49681 agcaaaggct atgttttaga ggcacaagat gacgaactat cgcgttgaga gtagcagcgg
    49741 acgggcggcg cgcaagatga ggctcgcatt aatgggacct gcgttcattg cggcgattgg
    49801 ttatatcgat cccggtaact ttgcgaccaa tattcaggcg ggtgctagct tcggctatca
    49861 gctactgtgg gttgtcgttt gggccaacct gatggcgatg ctgattcaga tcctctctgc
    49921 caaactaggg attgccaccg gtaaaaatct ggcggagcag attcgtgatc actatccgcg
    49981 tcccgtagtg tggttctatt gggttcaggc agaaattatt gcgatggcaa ccgacctggc
    50041 ggaatttatt ggtgcggcga tcggttttaa actcattctt ggtgtttcgt tgttgcaggg
    50101 cgcggtgctg acggggatcg cgactttcct gattttaatg ctgcaacgtc gcgggcaaaa
    50161 accgctggag aaagtgattg gcgggttact gttgtttgtt gccgcggctt acattgtcga
    50221 gttgattttc tcccagccta acctggcgca gctgggtaaa ggaatggtga tcccgagttt
    50281 acctacttcg gaagcggtct tcctggcagc aggcgtgtta ggggcgacga ttatgccgca
    50341 tgtgatttat ttgcactctt cgctcactca gcatttacat ggcggttcgc gtcaacaacg
    50401 ttattccgcc accaaatggg atgtggctat cgccatgacg attgccggtt ttgtcaatct
    50461 ggcgatgatg gctacagctg cggcggcgtt ccacttttct ggtcatactg gtgttgccga
    50521 tcttgatgag gcttatctga cgctgcaacc gctgttaagc catgctgcgg caacggtctt
    50581 tgggttaagt ctggttgctg ccggactgtc ctcaacggtg gtggggacac tggcggggca
    50641 ggtggtgatg cagggattca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac
    50701 catgttgccg tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat
    50761 gagtcaggtg ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac
    50821 cagtgacagc aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg
    50881 ggtgattgta gtgctggtcg tggcgctgaa tatctggttg ttggtgggga cggcgctggg
    50941 attgtagttg aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat
    51001 ccgccctacg cgttaaagag ccggtttgta ggcctgataa gactcgctag cgtcgcatca
    51061 ggcattgtgt tccattggcg aatgtgttta atggcggtgt ccatgtccgc ggccttttcc
    51121 gcgatgatcg tcacgatcgc gccagccttc acgatagcca cgctcatacg ctttacgctt
    51181 atcccagcca cgatgatagc cattatcatg acgccaccag cggtttttac gccactcata
    51241 attgcgatgc cagtagtcac ggtcgcgcca gtgaccaccg tcccagtaat taccgtaatg
    51301 atcgcgatcg ccaatttgta atttgattga tggcagtagg gtgacttcac ctgcgtttgc
    51361 tgcaagcggg gtaaatgcca tcagggcggc cgccagaaac agtgacctga acattgttat
    51421 tctccttcac gatcgaagcc gtcagcggcc tgttaacgca atattacggg gaggtaaagc
    51481 cccgtatcat cgccataact cttaaatcat aaaggagagc attttttgct aaacattatc
    51541 gtggggtggc tgttgcttcg gacgattgac catcatcatc ggcttacctg ctaataacag
    51601 ccatgccgga agcatgacga tacagagcag cggtagcaat gtggtattgg gtacaacgac
    51661 tgccgccata aaaagactta accagccatc gcgtgttacc accagcacga tgccgagaat
    51721 agcgcaggaa acggttattg ccgctggaac ggcgggaacg tgctcatgca gcatcaaccc
    51781 taatgccacg ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc cactggcggc
    51841 agcaacgacc agggcggcaa gtttaattac cgccagcaaa aagtaatcgc tggtgctgaa
    51901 agcctgattt gccaccatct gctgcatctc atccagccct ttaaacagcg aaactggtcc
    51961 accaataacc cccagaatac cgagaataaa tccgccaata cccagcacga gcaccggatt
    52021 tttcatttga tgcatcattg agtgcaaccg tggtaagcac catacggcga ccatccctgc
    52081 tgcgatggca atcgccgcga caattgcacc gctgagaata tcggtcattt ccatctgtcc
    52141 gtaatgcgca atgggcagtg aaaaatgagg atggaaaaat aatccggtag taagtgcacc
    52201 agctgctgcc gccattaacg gcgcaaagag acgatcccat agcggaactt cactactgcc
    52261 atttaaggtt tgcgaaaata tcaacgccgc cgcaacaggt gtgccaaaca gcgcgccgat
    52321 ggttccggca gaggctaaaa tagtccactc cattcggttg acgcgcggta acagacgagc
    52381 gccaatggca accgcaaggg cgatattgac ggtcatgatc ggatgttctg gccccaggct
    52441 gacgccacca gcaagaccaa gaattaatgc tacgataagt cctggtagcg cagagggcgg
    52501 aaccggtgcg ccgatcagcg gttcacaggc ggggtctggt ccggcatgac cctggctgaa
    52561 acggataacc aaccccaccg caatacccgt tagcgttaat acaccgatga tccagagggg
    52621 tgaatcctgg gctatcccca gagttcccgg cagttgttgc cagagcaaat tctgtaatgc
    52681 cgaggcgatt ttcatcacca caatcagaat aagactggac gcaatcccaa ttgccacggc
    52741 ggggagcgat aataacaaca tggttctggc tcgcggatgg agcatgttct attccttatg
    52801 cggggtcaga tacttagttt gcccagcttg caaaaaggca tcgctgcaat tggtgctgaa
    52861 acgataaagt aattgtgtga cccagatcga tatttacagg gagcctgcct ttccggcgtt
    52921 gttgttatgc ccccaggtat ttacagtgtg agaaagaatt attttgactt tagcggagca
    52981 gttgaagaat gacaaagtat gcattagtcg gtgatgtggg cggcaccaac gcacgtcttg
    53041 ctctgtgtga tattgccagt ggtgaaatct cgcaggctaa gacctattca gggcttgatt
    53101 accccagcct cgaagcggtc attcgcgttt atcttgaaga acataaggtc gaggtgaaag
    53161 acggctgtat tgccatcgct tgcccaatta ccggtgactg ggtggcaatg accaaccata
    53221 cctgggcgtt ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat ctggaaatta
    53281 ttaacgattt taccgctgta tcaatggcga tcccgatgct gaaaaaagag catctgattc
    53341 agtttggtgg cgcagaaccg gtagaaggta aacctattgc ggtttacggt gccggaacag
    53401 ggcttggggt tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg ccgggcgaag
    53461 gcggtcacgt tgattttgcg ccgaatagtg aagaagaggc cattatcctc gaaatattgc
    53521 gtgcggaaat tggccatgtt tcggcggaac gcgtgctttc tggccctggg ctggtgaatt
    53581 tgtatcgcgc aattgtgaaa gctgacaacc gcctgccaga aaatctcaag ccaaaagata
    53641 ttaccgaacg tgcgctggct gatagctgca ccgattgccg ccgcgcgttg tcgctgtttt
    53701 gcgtcattat gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca tttggcggcg
    53761 tgtttattgc gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc tctggtttcc
    53821 gtgccgcatt tgaagataaa gggcgcttta aagaatatgt ccatgatatt ccggtgtatc
    53881 tcatcgtcca tgacaatccg ggtcttctcg gttccggtgc acatttacgc cagaccttag
    53941 gtcacattct gtaaatcctt cctgttatat cgggaggtaa ctctcccgat aatcttttaa
    54001 atcatacagt taattcaatt tttctttgtg tcccctcaca aggccgacct gcgtcacact
    54061 tccatacagc gggattaatt ctccagtaaa tgcattattt gtctggtaac ggcgatttgt
    54121 tttgcacgtt cataatttca ctcgtcaact atcactgaac gaggaattaa cgatgagtaa
    54181 gaaactgatt gccttatgtg cctgcccgat gggcctggct cacactttta tggccgctca
    54241 ggcgctggaa gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc
    54301 ggacggtatc cagaatcgcc tgacggcgca ggatatcgcc gaagcaacca tcatcatcca
    54361 ctccgtggca gttaccccgg aagataatga gcgtttcgaa tcacgcgacg tttatgaaat
    54421 cactttgcag gacgcaatta aaaacgctgc gggcatcatc aaagaaatcg aagagatgat
    54481 tgcctctgaa caacagtaat attcgacagg gataattgtt atggccatta aaaaacgcag
    54541 tgcaaccgtt gtgcctggcg catccggtgc ggcagcggca gttaagaatc cgcaggcctc
    54601 taaaagcagc ttctggggtg aactcccgca gcatgtgatg tcagggattt cacgcatggt
    54661 gccaacctta attatgggcg gtgtgatcct cgctttcagc cagctgattg cttatagctg
    54721 gcttaaaatt cctgctgaga tcggcatcat ggatgctctt aatagcggga aattctccgg
    54781 tttcgacctc tccttactta aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt
    54841 tggttttgcc attccgatgt ttgctgcttt tgtggcgaac tctatcggtg gcaaactggc
    54901 gttcccggct ggttttatcg gcgggttgat gtctacccag ccgacgcaac tgctgaactt
    54961 cgatcccagc actatgcaat gggcgacctc ttcgccggtg ccgtcaacct tcattggtgc
    55021 gctgattatt tctatcgtcg cgggttacct tgtgaagtgg atgaaccaga aaatccagtt
    55081 accggatttc ctgttagcgt tcaaaaccac atttttgcta ccgattcttt ccgccatttt
    55141 tgtcatgctg gcgatgtact acgtcatcac cccctttggt ggctggatca acggcggtat
    55201 ccgtactgtg ctgactgccg caggtgagaa gggcgcgctg atgtacgcaa tggggatcgc
    55261 cgcggcgaca gcaatcgacc ttggtggccc gatcaacaaa gccgcaggtt tcgttgcctt
    55321 cagctttacc actgaccacg tattaccggt caccgctcgt tctatcgcta tcgttattcc
    55381 tccgattggt ctgggtctgg cgaccattat tgaccgtcgt ttaaccggca aacgcctgtt
    55441 caacgctcaa ctttatccgc agggtaagac cgcgatgttc cttgccttta tggggattag
    55501 tgagggtgcg attccgtttg cgctggaaag ccccatcacc gccattccgt cgtatatggt
    55561 cggcgcgatt gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga
    55621 atccgctatc tgggcatggc cgctggtcac taacctgggc gtctatatgg cggggatcgc
    55681 gctgggggca gtcataactg cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa
    55741 aggcaaattg ttaatcgata gcctgtaaga aggaaaatcc agatgacatt actcgcttcg
    55801 ctgcgcgact ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc acggcagaac
    55861 aaacagccgc atctggggat ctccaccgga tcaggctatg tggttattag ccgtgaaagt
    55921 gcgcacattc tggtagattc gcgctatttc gttgaggtgg aagcccgtgc gcaaggctac
    55981 cagctgcatt tgcttgacgc gacgaacacg cttaccacta tcgtcaatca aatcattgcc
    56041 gatgagcagt tgcaaacgct cggttttgaa ggccagcagg tgagttggga aaccgcgcac
    56101 cgctggaagt ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa
    56161 atcaaaacgc cagaggaggt ggagataatc cgcctcgcct gtgggattgc cgatcgcgga
    56221 gcggagcata ttcgccgctt tattcaggcg gggatgagcg agcgcgagat agccgctgaa
    56281 ctggagtggt ttatgcgcca gcagggcgca gaaaaagcct cttttgacac cattgtcgcc
    56341 agcggctggc gcggggcgct gccgcacggc aaagccagcg acaagattgt tgcagcgggc
    56401 gagtttgtca ctctcgattt tggtgcgctg tatcagggct actgctctga tatgacgcgc
    56461 accttgctgg tgaatggcga aggggtgagc gccgaatctc acccgctgtt tgacgtctat
    56521 cagattgtcc tgcaggcaca gctcgcggca atctctgcaa ttcgccccgg cgtgcgctgc
    56581 cagcagattg acgacgccgc gcgccgggtc attaccgagg ctggatttgg cgactatttc
    56641 ggtcataaca ctggtcacgc tatcggcatt gaagtccatg aagatccgcg tttttcaccg
    56701 cgggacacca cgacgctaca gccaggcatg ttactgaccg tggagccggg gatttatttg
    56761 ccagggcaag ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca aggcgcagaa
    56821 gtgctctacg ccatgccgaa aacagtgttg ctcacgggag aggcataatg gatttatcgc
    56881 tattaaaagc gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga
    56941 tcctgctgga agaagcggat cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg
    57001 tgctgatccg cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc catatggacg
    57061 aagtgggatt tatggtgcgt agcatctccc gcgaaggggc aattgatgtg ctgccggttg
    57121 gcaacgtgcg catggctgcc cgccagctgc agccggtgcg catcaccacc cgtgaagagt
    57181 gcaaaattcc aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc gccatgcgcg
    57241 tggacattgg tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc
    57301 gcgtcacctt tgatacaact tttcaggttc tccctcacca gcgggtgatg gggaaagcct
    57361 ttgatgatcg cctcggttgc tatctgctgg tgacgttact acgtgagctg cacgatgccg
    57421 aactgcctgc ggaggtgtgg ctggtggcaa gttccagcga agaggtggga ttacgcggtg
    57481 ggcaaaccgc tacccgcacg gtgtcgccgg acgtcgccat tgtgctggat accgcctgct
    57541 gggcgaaaaa ctttgattat ggcgcggcta accatcgcca gattggtaac ggcccgatgc
    57601 tggtgttaag cgacaagtca ctgattgcgc cgccaaaact taccgcctgg atcgaaaccg
    57661 tggcggctga aattggcgta ccgttgcagg cagatatgtt cagcaacggc ggcacggacg
    57721 gcggagcggt gcacttaacc ggtactggcg tacccacagt ggtaatgggg cctgccaccc
    57781 gccacggaca ttgcgcggca tcgattgccg attgccgcga cattttgcag atggagcaac
    57841 ttttatctgc ccttattcaa cgtcttacgc gtgagacggt tgttcaactg acggatttca
    57901 gatgatctcc tgattaaccc ggagcggtta tgttaacgat tcaatttctc tgtcctttgc
    57961 ctaacggtct acatgctcgt ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa
    58021 gcgaaatcac ctttttcaac catcgccaga acgcaaaggc cgatgctaaa agctcgctgg
    58081 cgttgattgg aactggcacc ttatttaatg acagttgcag cctgaacatt agcggcagcg
    58141 atgaagagca ggcgcggcgc gtgctggaag agtacatcct ggtgcgcttt atcgacagcg
    58201 acagcgttca gccgacgcag gcagaactga cggcgcatcc gctgccgcgt tcattaagcc
    58261 gccttaaccc ggatttactg tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc
    58321 tgaccctgtt acaaagcgac agcctcgaca gttatcgggc aatccccgcc agtgcgcaag
    58381 attccacccg gctggagcac agcctggcaa cgcttgccga gcaactgaac cagcaattgc
    58441 gtgagcgtga cggcgaaagc aaaactatcc tcagcgccca tttgtcgctg attcaggatg
    58501 atgaatttgc aggcaatatc cgtcgcctga tgacagaaca gcatcaggga ctgggggcgg
    58561 cgatcatcag caatatggag caggtttgcg ccaagctttc tgcttctgcc agcgattatc
    58621 tgcgcgaacg tgttagcgac attcgcgata ttagcgaaca gttgctgcat atcacctggc
    58681 cggaactgaa gccgcgcaac aatctggtgc tggaaaaacc gaccattctg gtggctgaag
    58741 atttaacccc aagccagttt ttgagccttg atttgaaaaa tcttgcgggc atgattctgg
    58801 agaaaaccgg gcgcacctcg cataccctga ttctggcccg cgcctcggcg atcccggtac
    58861 tgagcggcct gccgctggat gcgattgccc gttatgccgg gcaaccggcg gtgcttgacg
    58921 cccagtgcgg cgtgctggcg attaacccga atgacgcggt gagcggttat tatcaggttg
    58981 cgcagacgct ggcggataaa cgccaaaaac aacaggcgca ggctgccgcg cagctggcct
    59041 attcccgtga taacaagcgt attgatatcg cggcgaatat tggcaccgct ctggaagcac
    59101 caggcgcgtt tgccaacggc gcggaaggcg tcgggctgtt ccgtaccgaa atgctgtata
    59161 tggatcgtga cagcgcgccg gacgagcagg agcaatttga agcctaccag caggtgctac
    59221 tggcagcggg cgacaagccg attatcttcc gcacaatgga catcggaggc gataaaagca
    59281 ttccttatct gaatattccc caggaagaga acccgttcct cggctatcgc gcggtacgta
    59341 tttacccgga atttgctggc ctgttccgca ctcaactacg ggcgattttg cgcgctgcca
    59401 gttttggcaa cgcccagttg atgatcccga tggttcacag cctcgatcag atcttatggg
    59461 tgaagggcga gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag
    59521 agacgattac tctgggtatc atggtggaag tgccgtcggt gtgttacatc atcgaccact
    59581 tctgcgatga ggtcgatttc ttcagtatcg gttctaacga tatgacccag tatctgtatg
    59641 cggtcgatcg taataacccg cgcgtatcgc cgctgtataa cccgattacg ccatcgttcc
    59701 tgcgcatgtt gcagcaaata gttaccactg cgcatcagcg gggcaaatgg gtaggcattt
    59761 gcggtgaact gggcggtgaa agtcgttatc tgccgctact gcttgggctg ggcctggatg
    59821 agctgagtat gagtagcccg cgcattccgg cggtgaaaag ccagctgcgt caactggata
    59881 gcgaggcgtg tcgggaactg gcacgtcagg cgtgtgaatg tcgcagcgcg caggaaattg
    59941 aagcgttact caccgccttt acgccggaag aagacgttcg cccactgctg gcgctggaga
    60001 atatctttgt tgatcaggat tttagcaata aagagcaggc gatccagttc ctgtgcggca
    60061 acctcggcgt taacgggcgt actgaacatc cgtttgagct ggaagaagat gtctggcagc
    60121 gggaagagat tgtcactacc ggcgttggtt ttggcgtagc gatcccgcac accaaatctc
    60181 agtggatccg tcattccagt atcagcattg cccggctggc gaaaccgatt gactggcagt
    60241 cagaaatggg cgaagtcgaa ctggtgatca tactgacgct gggtgctaac gaagggatga
    60301 atcatgtgaa agtcttctcg cagctggcgc gtaaactggt gaataaaaac ttccgccagt
    60361 cgttgtttgc cgcgcaagat gcacaaagta tcctgacgct gctggaaaca gaattaacct
    60421 tctgacgtta gccctgaaaa cgggcgctgt actctcccgg cgtcagacca aactgacgcc
    60481 ggaaaacgcg acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa
    60541 ttgacagatg atatttctgc aaaatcatcc tcgccttcgc cattcgcacc caacgcacat
    60601 actcgataaa acgcatcgtt ccatgctgag caaacagttt tgacagatga ttgggcgtga
    60661 tattaaaaaa ctgcgccacg ctctcgcggg tgagcggctg ggcataatta tcctgaaccc
    60721 agttacaaat gctgtgatag agaaaatcgc cgcgcggttg cgaattaccc ggcggcatat
    60781 tcaccacgtt gcggcaaaga tgcagcaaac ttaacaccag tggctgaatg atattctgct
    60841 ccagcggcga acggctaaga tgggtgaggg cggtaagcat cgcttcacct tcaccgcgtt
    60901 gcaggctggg aagctggatc tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt
    60961 aaaacgataa cccaagccag gtcggtgcga acaccaggct taacagcatc accggtttgt
    61021 tgttgatcgg taaattagcc gcgcgtgccg gaataaacag catttctcct tccaccattc
    61081 gctgctgctt tgcttccagc aggttgccgt attcgccgcg cagcacgata tccagacggg
    61141 gaaaatcaat acataaactg cccaccggta acgaggcagg ctggctggca aaccagacgc
    61201 gccccagtag ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa aattgttgat
    61261 cggcgggcaa gcctggtgct ttcatgaaag ttcccgatta aagatgcatt aactggcgaa
    61321 attctttcac tttgctgcgg ctgaccggca cttcaaaatc cagatctttc agtcgcagaa
    61381 tgtaggtgtt attaaaccac ggttcgattt cgcgtatttt gttcagattg acacaaaatg
    61441 agcgatggca gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca
    61501 tcggcattac gtaggattca cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg
    61561 cgtaatagat atcgttaatt ggcgtgacga ttattcgctc atctttcacc agattaatgg
    61621 tgtcattctc acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg
    61681 ccgcttccag tttttgcagc atcccggtaa tacgtgactc ctgatacggt ttgagaatgt
    61741 agtcaaacgc ctccagttca aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca
    61801 caataaacgg tttatgggcg aactggctga tgttttgcgc cagcaacacg ccatccagcg
    61861 acggaatatt gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca
    61921 cgtccagacc gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct ttaattagcc
    61981 agctcagttc ctgttgtgcc aggaattcgt cttcaacaat gatgactttc acaatatcac
    62041 tccggctcaa agcaataacg tagcctgtga ggcgactggg gtgcgttggt taggaatgta
    62101 aaacgcaatt tccgtccccg gctccaggcg gcggatatgc agcccctcgc catacaataa
    62161 cttcacgcga tgatggacat tcagcaggcc aattttattg cccggcattt cattcgcttc
    62221 gacccgctca atcacctttg gatcgatgcc gtgcccggta tctcgcaccg caatgcgtac
    62281 ccgatttccg cactctgcaa cgctgatggt gacaacgcct ttacctttgc aaggctgaat
    62341 accgtggaca atggcgttct ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt
    62401 cacctcttca tcaatatcat agataaccgt cagcttgtca ccaaagcggg cctgctcaat
    62461 ggcaatatag tctttaattt gatacagctc ttttttgata tcgatttgct catcgtcttt
    62521 taattcaatg ttatagcgca gataacgcga cagattaaag atcaactggc gagcggtatc
    62581 cggattcaga cggattgacg atgaaatagc gttcagagcg ttaaacagaa aatggggatt
    62641 aattttgctt tgcagggcgc gaagctctgc cttatttgcc atttcacgta gctgctcggc
    62701 gcgtgaaacc tccagttgcg tggagatgat ttgcgacaga ccgaccgcca tttcctgtaa
    62761 tgacgaggtg atctgatgcg cgtggcagta gtaaattttc agcgttccgg tcacgacccc
    62821 tttctcccac aatgggatca ccagcatgga atgaatctct ggtgtgcggt gggcttcatc
    62881 attgttttta atgatgattt ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa
    62941 gtcatcgcca ttctgatagt tatgttcacc cacgccaaca taggccagca catgatcggt
    63001 attggtaatt gccaccgcat cggcgtgaat gtcatcgcgg ataatttcgc agaccttgcg
    63061 taatgactcg ctattgacat ggcgaaacag cggcagcgtt ttgttggcga tatccagcgc
    63121 cagcttggcc tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt gcaccagaag
    63181 cacaataaag ccgatacaga cgctaccgag gatcattgga atgccgattt tagagacgat
    63241 atcgatcccc aacgcggtag ttggtgccca gacaattacc agaatcatgg tcaacgtctc
    63301 acacaacatg ccgccgagaa taccgacgcg ccagcgctgt gctttgggga ttttcaggtt
    63361 tatccagcct gatatacaac ccgccagaat gctggtgata aagcagggga tcgccgtcac
    63421 gccgccgata tcaattaaat accggtgaat acccgcaatc acgccggtaa tgatgcctac
    63481 ccacgggcca aacagaatcc cgccggacat caccgcgata atacgtacgt tcaccagcga
    63541 gccttctacg ggaacgccgg accaggtgct gaacagggcg aacagcgaga aaatggcggt
    63601 aacggcgagc aattcttttg gggagtgcgc cgacttgtgc aacagttcgc gaaacaggcg
    63661 gatacggatg agaaagaaca ggcagataag cattaacgct gcccgatcga agaccgccag
    63721 cagcatgttg aatatttcgt gcacgggtga ctctgaataa ggattaaacc tctatgatag
    63781 gtaacctgaa ggctgatgac cagcaggccg tttttgagga aaaaccacaa ttttaaaagg
    63841 ggattgaaag ataaatggtg tattagcaat gcatctcttg ataatgcact tctggttagt
    63901 aaatttattg aaattattat gttaataaaa tgagtggtga ttttcttgtt agcgcagata
    63961 aatttcctgt ttttaaagca gagttcctca tttaacgtca ttaataaaaa tgttctaaaa
    64021 cgctggacag acagaaatta atcaggctat tttcaaaaca gccaccatcg tggcagcgtc
    64081 gctcggacgg tccgggcgct aacgttaatc tgaggaaatt atggctgaca ctcgccctga
    64141 acgtcgcttt acgcgcattg atcgtctccc gccctatgtt tttaacatta ccgctgaact
    64201 gaaaatggct gcgcgtcggc gcggcgaaga tattatcgat ttcagtatgg gtaacccgga
    64261 cggtgcgact ccaccgcata tcgtcgaaaa attatgtact gtggcccagc gcccggacac
    64321 gcatggttac tccacttcac gcggcattcc gcggctacgt cgcgccattt cccgctggta
    64381 tcaggatcgc tacgacgttg aaatcgaccc ggaatcagaa gccatcgtca ctattggttc
    64441 gaaagagggc ctggcgcatc tgatgctggc gacgctggat catggtgaca cggtgctggt
    64501 gccgaatcca agttacccga tccatattta tggcgcggtg attgccgggg cgcaggtacg
    64561 ctcagtgccg ctggtggaag gtgtcgattt cttcaacgaa ctggagcgcg ccattcgtga
    64621 aagttatccg aaaccgaaga tgatgatcct cggcttcccg tcgaatccaa ccgcgcaatg
    64681 cgtggagctg gagttcttcg aaaaggttgt ggcgctggcg aaacgctacg atgtgctggt
    64741 ggtccacgac ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc cgtcaatcat
    64801 gcaggtaccg ggtgcacgcg atgtggcagt cgagttcttt acgctgtcga aaagctacaa
    64861 catggcgggc tggcgtatcg gctttatggt tggcaacaaa acgctggtca gcgccctggc
    64921 tcgtattaaa agctatcacg attacggcac ctttacgccg ttgcaggtgg cggcgattgc
    64981 ggcgctggag ggcgatcaac agtgcgtgcg cgacattgcc gaacagtaca aacgccgccg
    65041 tgatgtattg gttaaagggc tgcatgaagc gggctggatg gttgaaatgc cgaaggcttc
    65101 gatgtatgtc tgggcgaaaa tcccggaacc atatgcggcc atgggatcgc tggaatttgc
    65161 caagaagctc cttaacgaag cgaaggtttg tgtctcgccg gggattggct ttggcgacta
    65221 tggtgatacg catgttcgct ttgcactgat tgaaaaccgc gaccgtattc gtcaggccat
    65281 cagagggatt aaagcgatgt tccgtgccga cggtttacta cccgccagca gcaaacatat
    65341 tcacgaaaac gcggaataaa caccgtcgtc agaaatgcaa acaggagcct gatggctcct
    65401 gtttttcatt gcacagtttg cttttagatc atcagggcaa aagtaccggc ccaaacgatg
    65461 accataaaag aaatgcccat aaagaaatat ttcactttac atctctccgc gtaattactg
    65521 ttacgcctga atgacttcat tacacctgat acagtgagtt gacaccgggc aaagcgacct
    65581 taaaagccct gtattttacc gtgggcatca ccccagaatt ctgagctaaa ctgctcgaga
    65641 cggcgctaat gtacgcctaa aactgaaacg agacaagagg cattttctta tttactttta
    65701 gtaacttaca agtgtctcat atcggcgaca gtttaatttc tgacggtaat aattagatat
    65761 tcagcgaagg aaaaaacctt aagaaaaaag ccataaaaac catgaggtta ttatggccga
    65821 tttgaggagg gaaagagtaa gagcagtttg ttaaatgtac aacgacgatt ctcccaccgg
    65881 gcgcgtttta aagcgacggt ggatccagag gtactgctcc ggtgcgcgca tgatctcttt
    65941 ctcgataatc ttgttcatat aggcagcggc ttgattttca tctgtcgggt agccttccat
    66001 ctctggggtg atgaacaaac gatatccgct gtaatccgct tttcttacca tcgttacggt
    66061 caacatggct gcgccagaga gacgggagag aacataggtg ccattggttg tggcgacatt
    66121 ttccaccgca aagaacggcg cgaaggagct gcctttacga ccataatcct gatcgggagc
    66181 aaaccatacc gcttcacctt tcttcagtgc accgacaatg ccgcgcagat tatttctgcc
    66241 gatcatcgct ttgttagagc gcatgcgccc acgggtctgc acccattcca tcagctgatt
    66301 attatgtgga cgataggtag ccatcattgg ttggcacagt cccatcacgc ggccgcccag
    66361 ttccagcgac ataaaatgga cgccgacaac cattacgccg cgattttgca tttgtgcgcg
    66421 tttaaggtta tccaaccctt caacatcaaa ccatttacgt acgcgactgt cgggccagaa
    66481 ccatgccatg ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat
    66541 cttctcgcgt tcttccgcag aatgctgcgg gaaacaaagt tccaggtttt tacgggcgat
    66601 agattcacga cgtttcagga atggtcgcgc cattgcgcca atacgcgtgc cgagaaagca
    66661 gagaacagga taaggaagct gtacccaaag ccagagtaca ccaagcccaa accatgtgag
    66721 ccagtagcga ggatgtagaa actcgcggga aaatttgcat tgtggaaaca tagttacctt
    66781 ctgtagatac gaattgttgt atcgcagaag attccatgtc gacagcggta cactcaattg
    66841 gttgggccat catgtacgac aatggttcct gtaaagagtg taaagactag tcaggacata
    66901 accacgagga gcatgaatgc ggtatctgta ctttatcact cgctgactgc tttcagctac
    66961 tgattattaa tcgtgcaaag taaatatttt tccgtacaca acagtcttgc atttaacatg
    67021 ctgttttcat tgatgcttag caaaaattgc cagaggttaa ggattgtctt actattaaag
    67081 tgactgaagt tacaggtttt ttagcgcggc aggaggttga gctatgattt atttatggat
    67141 gtttctcgcc ctgtgtattg tctgcgtaag cggctacatc ggtcaggtac tgaacgtggt
    67201 atcggcggtc tcttctttct tcggcatggt gatcctcgct gcactcattt attacttcac
    67261 catgtggtta actggcggta atgaactagt gacggggata tttatgtttc ttgccccggc
    67321 ttgtggcttg atgattcgct ttatggtggg gtatggcagg cgatagtggg tcaggatggc
    67381 tcatttgggc aggtgttcac atcagcatca gacatgattg ttcacctgcc atgtgacaga
    67441 cgctatcgca caggatgaat aagataattc gcctttgtcg gataagtagg cgtgatttct
    67501 tctgtgaggt ttaatggtgg cgcatcttct ctcatcttaa acaaaatcgc ctgtgatggc
    67561 tgaagttttg tagagtgtgt ggttgtcatc gctgatgttt tttcagtctc tttatagctc
    67621 ctctcactta caccaccaga aatggaaaat gcaaaaacat tgcatgccat aatagagacc
    67681 aataaaaata agctgaaaag tattaagttt actttcatag aaagttatcc tgaaaatggt
    67741 tcactgagaa cctacg
//
LOCUS       DAMZII010000007        65625 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZII010000007 DAMZII010000000
VERSION     DAMZII010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.007"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     repeat_region   57..1000
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=57..85
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
     gene            complement(1340..2011)
                     /gene="queE"
                     /locus_tag="POA77_000417"
     CDS             complement(1340..2011)
                     /gene="queE"
                     /locus_tag="POA77_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI6363906.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            2150..2290
                     /locus_tag="POA77_000418"
     CDS             2150..2290
                     /locus_tag="POA77_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6363907.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            2304..3176
                     /locus_tag="POA77_000419"
                     /pseudo
     CDS             2304..3176
                     /locus_tag="POA77_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(3236..4534)
                     /gene="eno"
                     /locus_tag="POA77_000420"
     CDS             complement(3236..4534)
                     /gene="eno"
                     /locus_tag="POA77_000420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6363908.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4622..6259)
                     /gene="pyrG"
                     /locus_tag="POA77_000421"
     CDS             complement(4622..6259)
                     /gene="pyrG"
                     /locus_tag="POA77_000421"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDI6363909.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6487..7278)
                     /gene="mazG"
                     /locus_tag="POA77_000422"
     CDS             complement(6487..7278)
                     /gene="mazG"
                     /locus_tag="POA77_000422"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDI6363910.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7349..7684)
                     /gene="mazF"
                     /locus_tag="POA77_000423"
     CDS             complement(7349..7684)
                     /gene="mazF"
                     /locus_tag="POA77_000423"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDI6363911.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVVPEELQLI
                     KAKINVLIG"
     gene            complement(7684..7932)
                     /gene="mazE"
                     /locus_tag="POA77_000424"
     CDS             complement(7684..7932)
                     /gene="mazE"
                     /locus_tag="POA77_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI6363912.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8010..10244)
                     /locus_tag="POA77_000425"
     CDS             complement(8010..10244)
                     /locus_tag="POA77_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDI6363913.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10292..11593)
                     /gene="rlmD"
                     /locus_tag="POA77_000426"
     CDS             complement(10292..11593)
                     /gene="rlmD"
                     /locus_tag="POA77_000426"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI6363914.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVIQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11650..14406
                     /gene="barA"
                     /locus_tag="POA77_000427"
     CDS             11650..14406
                     /gene="barA"
                     /locus_tag="POA77_000427"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDI6363915.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14638..15978)
                     /gene="gudD"
                     /locus_tag="POA77_000428"
     CDS             complement(14638..15978)
                     /gene="gudD"
                     /locus_tag="POA77_000428"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI6363916.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQSDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(15999..17339)
                     /locus_tag="POA77_000429"
     CDS             complement(15999..17339)
                     /locus_tag="POA77_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI6363917.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17341..18693)
                     /gene="gudP"
                     /locus_tag="POA77_000430"
     CDS             complement(17341..18693)
                     /gene="gudP"
                     /locus_tag="POA77_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDI6363918.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19128..19577)
                     /locus_tag="POA77_000431"
     CDS             complement(19128..19577)
                     /locus_tag="POA77_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDI6363919.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19595..20377)
                     /gene="truC"
                     /locus_tag="POA77_000432"
     CDS             complement(19595..20377)
                     /gene="truC"
                     /locus_tag="POA77_000432"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDI6363920.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20377..20706)
                     /locus_tag="POA77_000433"
     CDS             complement(20377..20706)
                     /locus_tag="POA77_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDI6363921.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21328..21873)
                     /gene="syd"
                     /locus_tag="POA77_000434"
     CDS             complement(21328..21873)
                     /gene="syd"
                     /locus_tag="POA77_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDI6363922.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            21941..22789
                     /gene="queF"
                     /locus_tag="POA77_000435"
     CDS             21941..22789
                     /gene="queF"
                     /locus_tag="POA77_000435"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI6363923.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22901..24265
                     /gene="ppnN"
                     /locus_tag="POA77_000436"
                     /gene_synonym="ygdH"
     CDS             22901..24265
                     /gene="ppnN"
                     /locus_tag="POA77_000436"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDI6363924.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            24822..26111
                     /gene="sdaC"
                     /locus_tag="POA77_000437"
     CDS             24822..26111
                     /gene="sdaC"
                     /locus_tag="POA77_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDI6363925.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            26169..27536
                     /gene="sdaB"
                     /locus_tag="POA77_000438"
     CDS             26169..27536
                     /gene="sdaB"
                     /locus_tag="POA77_000438"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDI6363926.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVIYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            27648..28403
                     /gene="xni"
                     /locus_tag="POA77_000439"
     CDS             27648..28403
                     /gene="xni"
                     /locus_tag="POA77_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDI6363927.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRNSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(28458..29606)
                     /gene="fucO"
                     /locus_tag="POA77_000440"
     CDS             complement(28458..29606)
                     /gene="fucO"
                     /locus_tag="POA77_000440"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDI6363928.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(29634..30281)
                     /gene="fucA"
                     /locus_tag="POA77_000441"
     CDS             complement(29634..30281)
                     /gene="fucA"
                     /locus_tag="POA77_000441"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDI6363929.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            30828..32144
                     /gene="fucP"
                     /locus_tag="POA77_000442"
     CDS             30828..32144
                     /gene="fucP"
                     /locus_tag="POA77_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDI6363930.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            32177..33952
                     /gene="fucI"
                     /locus_tag="POA77_000443"
     CDS             32177..33952
                     /gene="fucI"
                     /locus_tag="POA77_000443"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDI6363931.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            34061..35479
                     /gene="fucK"
                     /locus_tag="POA77_000444"
     CDS             34061..35479
                     /gene="fucK"
                     /locus_tag="POA77_000444"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDI6363932.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            35481..35903
                     /gene="fucU"
                     /locus_tag="POA77_000445"
     CDS             35481..35903
                     /gene="fucU"
                     /locus_tag="POA77_000445"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDI6363933.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            35961..36692
                     /gene="fucR"
                     /locus_tag="POA77_000446"
     CDS             35961..36692
                     /gene="fucR"
                     /locus_tag="POA77_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDI6363934.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(36736..37836)
                     /gene="rlmM"
                     /locus_tag="POA77_000447"
     CDS             complement(36736..37836)
                     /gene="rlmM"
                     /locus_tag="POA77_000447"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDI6363935.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(37829..38224)
                     /locus_tag="POA77_000448"
     CDS             complement(37829..38224)
                     /locus_tag="POA77_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI6363936.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(38243..39160)
                     /gene="gcvA"
                     /locus_tag="POA77_000449"
     CDS             complement(38243..39160)
                     /gene="gcvA"
                     /locus_tag="POA77_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDI6363937.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(39511..39738)
                     /locus_tag="POA77_000450"
     CDS             complement(39511..39738)
                     /locus_tag="POA77_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDI6363938.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            39930..41135
                     /gene="csdA"
                     /locus_tag="POA77_000451"
     CDS             39930..41135
                     /gene="csdA"
                     /locus_tag="POA77_000451"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDI6363939.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            41135..41578
                     /gene="csdE"
                     /locus_tag="POA77_000452"
     CDS             41135..41578
                     /gene="csdE"
                     /locus_tag="POA77_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDI6363940.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(41629..42435)
                     /gene="tcdA"
                     /locus_tag="POA77_000453"
     CDS             complement(41629..42435)
                     /gene="tcdA"
                     /locus_tag="POA77_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDI6363941.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(42512..43609)
                     /gene="mltA"
                     /locus_tag="POA77_000454"
     CDS             complement(42512..43609)
                     /gene="mltA"
                     /locus_tag="POA77_000454"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDI6363942.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            43818..43894
                     /locus_tag="POA77_000455"
     tRNA            43818..43894
                     /locus_tag="POA77_000455"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43852..43854,aa:Met,seq:cat)
     gene            43928..44004
                     /locus_tag="POA77_000456"
     tRNA            43928..44004
                     /locus_tag="POA77_000456"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43962..43964,aa:Met,seq:cat)
     gene            44038..44114
                     /locus_tag="POA77_000457"
     tRNA            44038..44114
                     /locus_tag="POA77_000457"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44072..44074,aa:Met,seq:cat)
     gene            complement(44189..45442)
                     /gene="amiC"
                     /locus_tag="POA77_000458"
     CDS             complement(44189..45442)
                     /gene="amiC"
                     /locus_tag="POA77_000458"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDI6363943.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            45674..47005
                     /gene="argA"
                     /locus_tag="POA77_000459"
     CDS             45674..47005
                     /gene="argA"
                     /locus_tag="POA77_000459"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDI6363944.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(47067..48893)
                     /gene="recD"
                     /locus_tag="POA77_000460"
     CDS             complement(47067..48893)
                     /gene="recD"
                     /locus_tag="POA77_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDI6363945.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(48893..52435)
                     /gene="recB"
                     /locus_tag="POA77_000461"
     CDS             complement(48893..52435)
                     /gene="recB"
                     /locus_tag="POA77_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDI6363946.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQASLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(52428..55316)
                     /gene="ptrA"
                     /locus_tag="POA77_000462"
     CDS             complement(52428..55316)
                     /gene="ptrA"
                     /locus_tag="POA77_000462"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDI6363947.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(55492..58860)
                     /gene="recC"
                     /locus_tag="POA77_000463"
     CDS             complement(55492..58860)
                     /gene="recC"
                     /locus_tag="POA77_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDI6363948.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(58873..59196)
                     /locus_tag="POA77_000464"
     CDS             complement(58873..59196)
                     /locus_tag="POA77_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6363949.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(59181..59588)
                     /locus_tag="POA77_000465"
     CDS             complement(59181..59588)
                     /locus_tag="POA77_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDI6363950.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVLL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(59585..60148)
                     /locus_tag="POA77_000466"
     CDS             complement(59585..60148)
                     /locus_tag="POA77_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI6363951.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVHQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(60139..60609)
                     /locus_tag="POA77_000467"
     CDS             complement(60139..60609)
                     /locus_tag="POA77_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI6363952.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(60793..61587)
                     /gene="thyA"
                     /locus_tag="POA77_000468"
     CDS             complement(60793..61587)
                     /gene="thyA"
                     /locus_tag="POA77_000468"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6363953.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(61594..62469)
                     /gene="lgt"
                     /locus_tag="POA77_000469"
     CDS             complement(61594..62469)
                     /gene="lgt"
                     /locus_tag="POA77_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI6363954.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(62620..64866)
                     /gene="ptsP"
                     /locus_tag="POA77_000470"
     CDS             complement(62620..64866)
                     /gene="ptsP"
                     /locus_tag="POA77_000470"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6363955.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(64879..65409)
                     /gene="rppH"
                     /locus_tag="POA77_000471"
     CDS             complement(64879..65409)
                     /gene="rppH"
                     /locus_tag="POA77_000471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDI6363956.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT        15951 a        17701 c        16014 g        15959 t
ORIGIN      
        1 tatccccgct ggcgcgggga actcatcacg ataacgctgc tgtgattcgt ccccgtcggt
       61 ttatccccgc tggcgcgggg aactcaagac gacgtgatcc gcaaagtcga aggcacgcgg
      121 tttatccccg ctggcgcggg gaactctgtt tttctgccgg tttaacacgg tgcgataacg
      181 gtttatcccc gctggcgcgg ggaactctgt ttctgtccca actgaacagc gccgatagtc
      241 ggtttatccc cgctggcgcg gggaactcga atgatcccaa atttgggtta cagaaccagt
      301 cggtttatcc ccgctggcgc ggggaactcg gacttaaaga tcgcatcaca gtactggagg
      361 gcggtttatc cccgctggcg cggggaactc taccggtgtg aattacagca ccaccgccac
      421 cccggtttat ccccgctggc gcggggaact ccaaacaggt cgacatgttt ggctaacagc
      481 taacggttta tccccgctgg cgcggggaac tccgataaca cagggccgtc aatgcggccc
      541 ttttcggttt atccccgctg gcgcggggaa ctcttttacc gtaacgcagc acaagacacg
      601 ctccgcggtt tatccccgct ggcgcgggga actccccaga tgacagcttg cctaaaacga
      661 aagcagcggt ttatccccgc tggcgcgggg aactctgctt tcctacgaca aatctggcga
      721 tgttgcgcgg tttatccccg ctggcgcggg gaactcctca agatagagcc tccgggtcga
      781 gccgcgatcg gtttatcccc gctggcgcgg ggaactcttg aactgttgat gttatcaaaa
      841 tatcagctcc ggtttatccc cgctggcgcg gggaactcgg cgaacaaccg acccctgttg
      901 gacccgacga cggtttatcc ccgctggcgc ggggaactcg cggaaaacag ccaaaataac
      961 gcaattaccg ccggtttatc cccgctggcg cggggaactc tgtaaacata acctattatt
     1021 aattaatgat tttttaagcc agtcacaatc taccaacttt atagtatcac acaaacaaca
     1081 catccattat gttaaagagc acttaatcca ttgattaaaa aggtaaatat ttaaaataac
     1141 tctatacaaa ctaaaatcta ccaaacttta ccgcaataat tttcactcca gcgaaaaatt
     1201 aatgccacag aatttgtaga gaatgctaat gattagccct ggatgggtaa aatggaggag
     1261 ttttcagagg cgacaaacaa tataaatgag tagaagtctt tacaggttcc acccatttat
     1321 aacgcttata aatgtttaat caggcaatat ttagatattt atgtgtttgc atcgacaaac
     1381 gccaattacg cgcaatgcag gtttcaatgc acaaacgtgt ggcatcatcc ttttggctaa
     1441 tcggctgcag tgcaatgact cgcggtttat catcggtcag cgttgccagt agttcatcca
     1501 gtgcttcaat atcacgtacg cgccccactg gatgcttgat ttcgttggct cgctccagtg
     1561 cctgtgacaa cacttcatag ccgccgcgca tgttcagctt tggtgatacg gtaacccagg
     1621 tattcggtgt gcagcgtact tcatgagttc cactggtttc gatctggcag ctaaaaccgt
     1681 tcttttcgag caggtcagtc agtggcagca aatcatgaat gcaaggctca ccacccgtaa
     1741 tcaccacatg ccgcgcggta tatccctgcc gaccaatgac agccagcaaa tcttcactgc
     1801 tcgccgcccc ccacttatca ctctctttgg tcttcgccag aatgctgaaa agggagactt
     1861 cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag ccaaccgggc
     1921 atccctgtaa acgaataaaa atggcgggga cgccggtaaa gtaaccctca ccttgcaggg
     1981 tctggaacat ctcgttaatc gggtactgca tagcattctc tgtgaagtgg ataattgtta
     2041 attattgcag atcctgccgc aacaatcatg tcttattaac attctgttac atgcagattt
     2101 aaagaaaaat gcgaaacata tcgttaataa ttaaagggag taacgtatta tgtcagaaga
     2161 aaataaagaa aatggattta atcatgtcaa aacattcacc aaaattatat ttattttttc
     2221 tgtattagtt tttaatgata acgaatctaa aattaccgat gccgccgtca atttatttat
     2281 ccagatttaa tgaggaattt ataatgcgct atttcatact aatgttcact ttcgtatgtt
     2341 cctttgttgc agcccaacca actattgtcc cacaattaca gtaacaagtt acggatctta
     2401 cgagtagctt aaactcacaa gaaaagaaag aactgacgca caagttagaa tctattttca
     2461 ataataccca agtgcaaatt gctgtattaa ttgttcccac aaccaaagac gagacaattg
     2521 aacaatatgc tacaagagtt tttgacaatt ggcgtttagg agatgccaaa cgtaatgatg
     2581 ggatactgat cattgttgcc tggtcagatc gtactgtccg catccaggta ggttatgggc
     2641 tggaagaaaa ggtaaccgat gctctggcta gagatatcat ccgtagcaac atgatacccg
     2701 catttaaaca acaaaaattt gctcagggat tagagctagc tataaacgct ttgaataacc
     2761 aactcacttc acaacaccaa tatccgacta atccttcaga aagtgaatca gcgtcttcca
     2821 gtgatcatta ttactttgct attttttggg tatttgcagt gatgttcttc cctttctggt
     2881 tttttcatca aggcagtaat ttttgtcgcg catgtaaaag tggagtctat atttcagcaa
     2941 tctatctttt agatttattc ctgttctccg ataaaatttt ttccattgct gtattttcct
     3001 tcttttttac tttcaccata tttatggtct ttacctgttt atgtgtactt cagaaaagag
     3061 catcaggtaa aagttatcat tcagacaaca gcggttccgg tggaggctca agtggcggcg
     3121 gatttagcgg cggtggcggt tcttctggag gtggcggcgc atccggccgc tggtaactca
     3181 accataaaaa atgccagccc ggaggctggc atttttaaat cagataaagt cagtcttatg
     3241 cctggccttt gatctcttta cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga
     3301 tacgaatcag ctggttgtat ttagcaacac ggtcagaacg gctcatagaa ccagttttga
     3361 tctggcctgc agcagtacca acagccaggt cagcgatggt agcgtcttca gtttcgccag
     3421 aacggtgaga gataactgca gtgtagccag catctttcgc catcttgatt gcagccagag
     3481 tttcggtcag agaaccgatc tggttgaatt tgatcaggat ggagttagcg ataccttttt
     3541 cgataccttc tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca accagctgga
     3601 ttttgtcgcc cagaactttg gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac
     3661 cgtcttcgat agaaacgatc gggtactgtt tggtcagttc ttccaggaag tgagtgaatt
     3721 cttcagaggt gaacgctttg ttgccttcgc cagccagaac gtatttacca tctttgtaga
     3781 attcagaagc tgcgcagtcc atcgccagag tgatgtcttt gcccagttca taaccagcag
     3841 ctttaacagc ttcagcgata acagccagag cttcagcgtt ggaacccagg ttcggcgcat
     3901 agccaccttc gtcaccaaca gcagtgttca tgcctttcgc tttcagaact tttgccaggt
     3961 gatggaaaac ttcagaaccc atgcggatgg cttctttcac agttttcgcg ccaaccggct
     4021 gaatcatgaa ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt
     4081 tcatcatcgg aaccggcata gagtatttgc ccggagtacc gttcagttca gcgatgtgct
     4141 cgtacagcgg cataccttta gcagctgcag cagctttggc gttagccaga gatacagcca
     4201 ggattgcgtt cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg atcatgatct
     4261 tgtcaatgcc agcctgatct ttagcatctt tgccaatcag cgcctgagcg atcgggccgt
     4321 ttaccgcagc aacagctttg gttacgcctt tacccaggaa acgggatttg tcgccatcgc
     4381 gcagttccag agcttcacgg gaaccagtag aagcacctga cggagcagct gccataccga
     4441 cgaaaccacc ctccagatgt acttcggctt caacagtcgg gttaccacgg gagtcgatga
     4501 tttcacgacc gatgattttt acgattttgg acattaggtt ttcctcaagt cactagttaa
     4561 actgaaactc cagacaaaca acgcgtaccc agggtacgcg ttgccgttct aactttttta
     4621 cttacttcgc ctgacgtttc tggaactcgc tggcggcttt cacaaagcct gcaaacagcg
     4681 ggtgaccatc acgtggagta gaagtaaact ccggatggaa ctggcaagcc acgaaccacg
     4741 ggtgattcgg aacttcgatg atctcgacca actgatcatc cccggaacgg cccgcaacgc
     4801 gcagacctgc atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat
     4861 gacgctcaac aattgtcggc gcattgtaca gctggcgaac caagctatcg tcaaccaact
     4921 ggcactgctg tgcgccgaga cgcatggtac cgccgagatc gctcttctcg ctacgaactt
     4981 caacgttgcc gttttcatcg cgccactcgg taatcagcgc cacaaccggg tacttacagt
     5041 ctggcacaaa ttccgtagag ttggcgttct ccatgttggc aacatggcga gcgtaatcaa
     5101 ttaacgccac ctgcataccc aggcaaatgc ccagataagg aatattgttc tcacgcgcaa
     5161 aacgcgcggt cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca ggtacgagga
     5221 ttgcgtccag acctttaagg atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca
     5281 gtttgatgtt gacgctgaca cgattcttca gcccaccgtg tttcagtgct tcgatcactg
     5341 atttataagc atccggcagt tcaatgtact tgccgaccat accgatggtg acttcactta
     5401 ccgggttcgc ttcttcgaag ataacctgtt cccattcgga cagattcgct tccgggcagt
     5461 ttaagctgaa tcgtttacaa atataatcgt ccagcccctg agatttcaac aggcccggaa
     5521 ttttatagat ggaatcgacg tctttcagag aaataaccgc tttttccgga acattacaga
     5581 acaatgcaat cttcgcacgt tcgttcgccg gaacagcgcg atctgaacga caaatcagga
     5641 tgtcaggctg gataccgatg gagagcagct cttttacaga gtgctgagtc ggtttggttt
     5701 tgacttcacc agacgctgcc atgtacggca ccagcgtcag gtgcataaac agagtgtgct
     5761 cacggccaat ttcaacagcc atctggcgaa tcgcttcgag gaacggcaag gattcgatat
     5821 cacctactgt accgccgatt tctaccagta ctacgtcatg accttcgcca ccttccagca
     5881 cgcgctcttt gattgcgtta gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt
     5941 caccgcggcg ttctttacgc agaacgtcag agtagatacg acccgtggtg aagttgttgc
     6001 ggcggctcat tttggtacga atgaaacgct cgtagtgccc caggtccagg tcggtttcag
     6061 cgccgtcttc agtaacgaac acttccccgt gttggattgg gctcatagta cctggatcga
     6121 cgttgatgta cggatccagt ttcatgatgg tcacattgag gccacgggct tcaagaatgg
     6181 ctgcgaggga ggctgcggca atgcctttac ccagagagga tacgaccccg ccggtcacaa
     6241 aaatatagtt cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg atggaataac
     6301 caggacggga aagcagtata cccgaacatg acctgtgcca caaactttca ttatccctcc
     6361 tcttcgccag cgcactattg aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt
     6421 tttgacgcaa atcaagcgct tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta
     6481 attcccttag agatcaattt cctgccgttt tacctgttgc cagacttctt ccattgtttc
     6541 gaggtcaaca cctgtcattt ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa
     6601 acgacgctca aatttttcgt tcgctttttg caatgcgatt tccgcttttg tccctaaatg
     6661 acgagccaga ttgaccgtgg caaacagcag gtcccccatt tcctcctcca gtttagcctg
     6721 gtcgacaaca gcctgccgcg cttcgtacat cacctcgtcg atctcttcgt agactttatc
     6781 gactaccgga ccaagcgtcg tccaatcgaa gccaacgttg gcgcaacgtt tctggatttt
     6841 ttgcgcacgc attaaagccg gtaaactacg aggaatatcg tccagcgccg aatgctgcgc
     6901 tttctgcgcg cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt cactactgtt
     6961 ttcggcagaa ctgtcagcaa aaacatgcgg atggcgacgc tctaatttat cgctaatcgc
     7021 agcgcaaata tcattaaagt caaagcgccc ttcttcctga gccatttgcg cgtaaaacac
     7081 cacctggaac agcagatcgc cgagttcacc gcgcagatcg tcaaaatctt cacgggcgat
     7141 ggcgtccagc acttcgtagg tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg
     7201 ctctttatcc cacgggcagc cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa
     7261 acggtcgatt tgattcatta gaatgtcctg aaaattgcgg gtctgtcagg tggaaacctg
     7321 tgaccagaat agaagtgagt tagtaacact acccaatcag tacgttaatt ttggctttaa
     7381 tgagttgtaa ttcctctggg acaaccgttc ctttcttcgt tgctcctctt gcccgccagg
     7441 cgatactttt tacctgatca gctaacgcta cgccatcacg ttcctgaccg gataaaacaa
     7501 cttcgaacgg atatcctttt gattgcgttg tacaaggaac acacagacac atacctgttt
     7561 tgttgttgta catgaaagga ctcaggacaa cagctggacg atgtccagct tgctcgctac
     7621 cttttgtcgg gtcaaaatca acccaaatca gatcgcccat atcgggtacg tatcggctta
     7681 ccattaccag acttccttat ctttcggctc tccccagtcg atattctcgt ggaggttttc
     7741 cggcgtgatg tcgttgacca gttcagcaag cgtaaatacg ggctctttac gcactggctc
     7801 aataattaat ttgccatcca ccaggtcaat cttcacttca tcatcaatat tcagattgag
     7861 cgcctgcatt aacgtagccg ggatccgcac cgccggtgaa tttccccaac gctttacgct
     7921 actgtggatc ataacccttt cctcaaaccg ctatcatatg tagatacagt atatatcaat
     7981 ctacattgta gatacgagca aatttcggcc taactcccgt gcaaccgacg cgcgtcgata
     8041 acatccggca cctggttgag tttacccagc acgcgcccca gcacttgcag gttgtaaatc
     8101 tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca
     8161 agcacgttca ccttctcgtt ggcgagaatg gtcgtgatat cacgtaacaa cccactacga
     8221 tcattagcca ccacgcggac caccagcgaa tatccggcgg agtagctctc accccatacc
     8281 gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg ccagttgttc gcaatcggcg
     8341 cggtgtactg aaataccgcg cccctgggta atgaagccga caatctcatc tccaggaatc
     8401 ggctggcagc agcgcgcgat gtggtgcatc aggttgccaa caccttcgac caccacgcga
     8461 ccgttatctt tactgcggtt ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg
     8521 gcggcgtcct gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc
     8581 tgattgagac ggatatcccc gccaccaatc gccgccagca actcgtcgac atcattaaag
     8641 ttgtaacgcg gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc
     8701 tcgtcgtcaa ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac
     8761 caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac
     8821 cagtcacggc tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc
     8881 agctggtagg tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg
     8941 acatcactgt ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg
     9001 acatcacctt tcggcgtaaa gacatacacc cggtcgtcaa agacctggct gcgtacttcg
     9061 tcgagcattt cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag
     9121 gcaatccggt cttcatgtcc cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc
     9181 cagtgcgcag caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc
     9241 tcaacggttt tgccacctgg ccccagaacc acggtatgaa tagactgata accgtttggt
     9301 ttcgggttag cgacataatc gtcaaactca tccggcaggt ggcgatagtg agtgtgcact
     9361 atccccagtg cggcataaca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca
     9421 tcaaacagct catcgaaggc gaggttcttt ttctgcattt tgcgccagat gctgtagatg
     9481 tgtttcggtc gaccatacac ttcagcttta acgccttcag ctttcatctc agcgcgcaga
     9541 tggccgacaa actcttcgat atagtgttcg cggtcgagac ggcgttcatg cagcagtttt
     9601 gcgatgcgtt tgtattcggt cgggtggaga taacggaagc agtaatcttc cagctcccat
     9661 ttcagttgcc caatcccaag acggttcgcc aacggcgcgt agatattggt gcactctttt
     9721 gccgccagta cgcgttcatc ttccggcgca tcttttactt cgcgcagatg agcaatacgc
     9781 tccgccagtt tgatgaccac gcagcgaaaa tcatcgacca tcgccaataa catccggcga
     9841 acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc
     9901 gccgccatat cacgcacgcc gtgaataagg ttaacaaccg acttaccgac gctctcacgc
     9961 agcacatctt cgctaactac gttggcatca gccagaggga acagcagcgc cgcccgcagc
    10021 gtgtcaatgt ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat
    10081 aacagactgg catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct
    10141 aagcactcac acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca
    10201 aattcaccag ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg
    10261 gaccagacct gccgaaatcg gcaaatcgta actatttaac gcgcgagaaa agtaccatcg
    10321 attccagatg tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc
    10381 ctgcttttaa taacgcttcg ctatcccgag ccagcgttgc aggattacag gatacataaa
    10441 ctatacgaat aggttccagt tttataattt gctgtataac acctgcggca cctgctcgcg
    10501 cggggtccag caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt
    10561 cttcaagatt ttcatgataa aacgtcacat tctgtaaacc attaagacgc gcattctgct
    10621 ggcctttttc caccagcgcc ggaacacctt ctacacccac cacactggca gcttgtgtcg
    10681 ccaatggcag tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt
    10741 gcacatccag ccattccaac gcacgcgcta ccattttttg gttcacaccc gcattgacct
    10801 gaataaaatc gcgcgggcta aaagttaagc gcaacccgtt tgagtcatac cagggcatct
    10861 caccagagac ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag
    10921 aatgcgaaaa gcgttccagt ttttcgcgat cagccgaact tagcggtgcg gtatggcgca
    10981 aaatcatcag cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgtatag
    11041 cttgtaagct gcccaggcat gccctgactt tgggcagcaa tgcttcaagt tggggcacta
    11101 aaatggggca ttgtttaacg tcgacaatgt cactggagcc cgctttgcga aagcccatct
    11161 gaagttgctg tgttttcggt aagtagttca aacttaaacg cgcgcgacgg cgatagcccc
    11221 agggaacatc ggcgatcact tcagagactt cgtgtttcat taatcgggcg agtgccgcac
    11281 ttttgcttcg ctgctgtaaa tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc
    11341 caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc gcttaaccgg cgcacgactt
    11401 tagcgcgggc gtactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgtggca
    11461 gtaatccagg aataaataac gctttgccgt tatgtcgcgc cacgccctga ccaaaagagt
    11521 cgaggtcgtt gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt
    11581 agaattgcgc cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa
    11641 cggaactcca tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg
    11701 accgtcctta ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg
    11761 cagcgtcaac tggaagatgc cggtgccagc attattgagc cgcttgcagt ttctactgaa
    11821 tatggcatga gcctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt
    11881 cgccattccg atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc
    11941 acctccaatt ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct
    12001 cgccagctca ctgtcactcg tgacggcgat attatgatcc tccgcacgcc gattatttct
    12061 gagagttact cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgttg
    12121 ggatatattg cgcttgagct ggatcttaaa tcggttcgct tgcagcaata taaagagatc
    12181 tttatttcca gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc
    12241 ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt
    12301 cgcgggcaac tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa
    12361 aacggtatca actcgatggc aatgtcgctg gctgcttatc acgaagagat gcagcacaat
    12421 atcgaccagg cgacgtccga tctgcgtgaa acgctggagc agatggaaat tcagaacgtt
    12481 gagttagatc tggcgaaaaa gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg
    12541 gcaaatatgt cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg
    12601 acgctgaaaa cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg
    12661 gcaaataatt tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagcaggt
    12721 aagctgattc tggaaagtat tccattccca ctacgcagca cgctggatga agtcgttact
    12781 ctgctggcac attcttctca cgataaaggg ttggaactga cgctcaatat taaaagcgac
    12841 gtgcctgata acgtgatcgg cgacccactg cgattacagc aaatcatcac taacctggtg
    12901 gggaatgcaa ttaaattcac cgagaatggc aacattgata ttctggtaga aaaacgtgcg
    12961 ctgagtaata ccaaagtgca gattgaagtg cagattcggg ataccggcat tggtattcct
    13021 gaacgcgatc aatcgcgctt attccaggcc ttccgacagg ctgatgccag tatttcccgc
    13081 cgtcatggtg gcaccggtct ggggctggtg attacacaaa aactggttaa tgaaatgggc
    13141 ggcgatattt cgttccatag ccagccgaat cgcggttcaa ctttctggtt ccacattaat
    13201 ctcgatctga acccgaacat tattatcgaa gggccatcca cccagtgcct cgccggtaag
    13261 cgcctggcct atgtcgaacc aaactccgca gcagcgcaat gcacgctgga tattttaagt
    13321 gaaacgccgc tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac
    13381 gacatgatgt tgttaggcat cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag
    13441 cgattagcga aagcggtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca
    13501 caagtcaatg cagaaaaact caagcaagat ggtatcggcg cgtgtctgct gaaaccatta
    13561 acacctacgc gcctgttgcc tgccctgacg gaattttgtc atcacaaaca aaacacgctt
    13621 ttgcctgtaa ccgatgaaag caagctggca atgacagtca tggcagttga tgacaacccc
    13681 gctaacctga aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc
    13741 gatagcgggc atcaggcggt tgaacgggcg aaacagatgc cgttcgattt gatcttaatg
    13801 gatattcaaa tgcctgacat ggatggcatt cgggcctgcg agctcatcca ccagctcccc
    13861 catcagcaac aaacgccggt tatcgcggta acggcgcatg caatggccgg gcaaaaagag
    13921 aagctgcttg gcgcagggat gagcgattat ctggcgaaac cgattgaaga agagcgattg
    13981 cataatttgt tgttgcgcta caagcctggc agcggtattt cctctcgcgt cgtgacgccc
    14041 gaagtcaacg aaattgtggt gaacccgaat gcgaccctcg actggcaact ggcactacgc
    14101 caggcagcag gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg
    14161 cctgaagttc gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt
    14221 gatttgattc ataaactgca tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat
    14281 ctctgccaac tgatcgaaca acagctacgt agcggtacta aagaagaaga tttggagccg
    14341 gagctgctgg aactgttgga cgagatggat aatgtagcgc gcgaagccag caaaattctc
    14401 gggtaacgga cgtcttgtcg agtagcaatc ggctttttat gacgctggtt tcagattggt
    14461 gacaaagtgc ctgtgtttat gccggatgcg gcgttaacgc cttatccggc ctacaaaacc
    14521 aattaaattc aatgaattaa aaaatgatgt agacctgata catagcacaa caggctattt
    14581 tgcgtttagc atcagtctca agccggctcc agatagagcc ggttttggtt ttctgtctta
    14641 acgcaccatg cacgggcgct tgttatcgaa cgtccagcca ggaatcagat actgcattcc
    14701 catcgcatcg tcacgcgcgc caagcccgtg tttctgatac agctcatggg ctttcatcac
    14761 ttgatccata tcgatttcta cacccagccc cggtttttcc ggcacctgaa ccagaccgcc
    14821 tttgatctca aacggttctt tggtcaggcg ctgattacct tcctgccaga tccagtgcgt
    14881 atcaatagca gtaattttac ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga
    14941 aatatcgaag tggttgtttg agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg
    15001 cgccacacgt accgaacctt gcatcgtcca gaaatgcgga tccgccagcg ggatatcaac
    15061 ggattgcagg gagagcgtat ggcccatctg ccgccagtcg gtggcgatca tattggttgc
    15121 ggtcggcagg ccagttgcgc gacggaactc tgccatcact tcacgcccgg agaaaccttg
    15181 ctccgcacca cacggatctt ctgcataagc cagcgaccct ttcaggtatt taccaatttt
    15241 aatcgcttcg ttcagcgacc aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa
    15301 gcgttgcgcc agtgccacaa tagactcggc ctcttcttcc ccggccagta caccgccttt
    15361 cagtttgaaa tcgttgaagc catatttttc atacgccgct tccgccaggc gcaccaccgc
    15421 atccggcgtc atcgcttctt catgacgcag gcgataccag tcgcatgagt catccgactg
    15481 gctttgatac ggcagcggtg tggctttgcg atcaccgacg aagaacagat aaccgagcat
    15541 ttcgacttcg ctacgctgtt gaccatcgcc cagcagggat gccacgttta cccccagatg
    15601 ctgccccagc agatccagca ttgccgcttc tatcccggta actacatgaa tagtggtacg
    15661 caaatcaaat gtctgcaaac cgcgcccacc agcatcacga tcggcaaaag tattacgcac
    15721 cagcgtcaga acgtttttgt attcacccag cgttttacct accaccagcg gaatcgcatc
    15781 ttccagcgtt ttacggattt tctcgccgcc gggaatttcc cctacgccag tgtgaccaga
    15841 attatctttg ataatcacaa tattacgcgt aaagaacggt gcgtgtgcac cactcagatt
    15901 catcagcata ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa caacaggcgt
    15961 cgtaaattga gaactcatat ttatgtcctt tttcagaatc aatgacggcc gaaaacggga
    16021 cgtttacggt caaaggtcca gccggggatc aggtactgca tcggacctgc gtcgttacgc
    16081 gcaccgacag gcagacgttt ataggcctca tgtgcctttt gtacctgttc ccagtccagt
    16141 tccacgccca gaccgggcgc atcaggaacg gcaatttttc cgtttttaat ctccagcgga
    16201 tttttggtca ggcgacaatc gccctcctgc caaatccagt gggtatcgat agcggtagga
    16261 ttacccggtg ccgccgcgcc cacatgggta aacatcgcca gagagatatc gaaatggtta
    16321 ttagaatggc agccccaggt cagcccccag tcgtcgcaaa gctgcgccac acggactgca
    16381 ccggaaagcg tccagaagtg cggatcggca agtggaatat ctaccgcatt gagcatcacc
    16441 gcatgaccca tttcgcgcca gttggtggcg atcatgttag tcgcgacggg caagccggtc
    16501 gcccgccgaa actccgccat cacttcacga ccggagaaac cctgttctgc gccgcatgga
    16561 tcttcggcat aggtaagaac atcattcagc cctttgcata gagaaatggc ttcatcaagt
    16621 agccaggcac cgttgggatc aacggtaatc cgcgcatcag ggaagcgttt cttcaatgcc
    16681 cgaacagtgt cgatttcttg ctcgccaggt aacaccccgc ccttaagttt gaaatcttta
    16741 aagccatagc gatcctgtga ggcttccgcc agacgcacaa cggcttcgct gttcatcgct
    16801 ttctgatggc gcaactgata ccactcatgg ttgcccggcg tattttccag ataaggaaga
    16861 tcggttttgg tccgatcacc gatataaaac agataaccga ggacggtaat agtctcgcgt
    16921 tgcttgcctg gccccaacag ttcgcagacc ggaacattca gcgccttacc tagcaggtca
    16981 agcaaggcgg cttccagcgc cgccacggca ttaacgcgca attcaaaagt ccaggcacct
    17041 ttgccgaagg tatcaaaatc ggctgcctga ttacctttat gcacctgctg aaccacttta
    17101 ttcaggcgcg caacttcctg gcccagaacc atcggaatag catcgaccag tgtttgataa
    17161 atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc
    17221 acaatattgc gggtgaaata tgcgttatgt gccccaccaa tattaagcaa catgctatcg
    17281 tgcccggcga ccggaatgac tttcatatca gtaataacag gactggattg tgtcgtcatc
    17341 ttattgcccc gcgacaggtt tcagctcgat acgcttgata tcgcccacca gcaccaggta
    17401 gctcagtacc gcgattaagg catgaacacc aacataaatc agcgccccat taaaggagcc
    17461 agtcgtgcca acgatataac caattgcgat tggcgtgacg atgccagaaa tgttaccgaa
    17521 catgttgaac aggccaccgg aaagaccgct gatctctttt ggcgcggtat ctgccattac
    17581 tgcccaaccc agcgccccga tgcccttacc gaagaaggcc agcgccataa agccgatgat
    17641 catccactca acgttgacgt agttgcagaa caccatcacc atcgacagca acatgcccat
    17701 tacgatcggt gttttacgcg caatgttcag cgatcccgtg cggcgcatca gccagtcgga
    17761 aataatccca cccagcacac cgccgataaa accgcaaacc gccggaacgg aagccacaaa
    17821 gcccgctttc agaatcgaca tcccacgcgc ctgcaccaga taaaccggaa accaggtaat
    17881 gaaaaagtaa gtcagggcgt tgatacagta ctgaccgata taaacgccga tcatcatccg
    17941 tgacccgagc agttgtttga tctgccccca cttcacgctg aacggcactt taactttggt
    18001 gttttgctga tccatattga tcagcgcgcc acccgcggcg atgtactcca gctctttctg
    18061 atttaccccc ggatgttgat ttggctcgtg gatgactttc aaccagataa agctgatgac
    18121 aatccccaga ccgcccataa agaagaagac gtgtgaccag cccacttcat gcgtcagcca
    18181 gcccataatc ggcgcgaaga tcaccgttgc gaagtattga gcggagttaa aaatcgacac
    18241 cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca atgcgactgt tgccggggaa
    18301 agatggcgct tcagcaagcc cgaccaggaa gcgcagagta aacagggcaa cgataatgcc
    18361 gaatccacta aagatatcga cgaagccttg cagcaaggta aacatcgacc agataaagat
    18421 cgaccagaag tagacgcgtt ttgaaccaaa acgatccagc aaccaaccac cagggatctg
    18481 cccgataaca taagcccatg agaaggcaga gaacacatag cccattccca cgggatcaag
    18541 gccgatatct ttggccattt ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt
    18601 gaaggatgtg acgataaaca acatcaccac tatccagtaa cgagcattcg tgcgtttttc
    18661 cacactgctc gcagcctgac ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag
    18721 ccacgctcac tctggactgc gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt
    18781 gccgttttta taggtcgttc gccgaatacg gcgcgtgttt atatctggca atagcagtat
    18841 aaaaagcgcg ccatagcggc tcaccgtgca acaacacaac attaatgcgt tcaatgaggc
    18901 ccgattttgg cattagccct ggacggtgga atccacttca cggaaatgaa aacaagaaca
    18961 agaaaggaag ggttaaaacg aagaaataaa gaagagtatg aaatggatcg cttgactcca
    19021 ggcaaacgcc agtaaaaatc cgcgctatga agcagttttt actggcattt gcctgaaaat
    19081 attcgattca gcaccgctaa aacgacattt accgctcgct gaacatatca ggacaacagc
    19141 gtgccccact gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg
    19201 tcaatcaaca gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac
    19261 tgtttgccgc cattgcagaa atttacataa ctgctgtcac cgagcgcaat cacgccataa
    19321 cgcagattcg gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta
    19381 tcaggaaggt cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag
    19441 ggcagccagt cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc
    19501 agaattgctt ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa
    19561 ataccaattt ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac
    19621 ccgatggcgc actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt
    19681 gtgataacgc ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag
    19741 taaaaggatg cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat
    19801 gctcagcacc gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat
    19861 gacgcaaatg ggcaagatgt cggcgaagct gatgtttgcg tccggttttc ggctccagtt
    19921 ccaccaggcc gtagcgtgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg
    19981 ccagaccgcg ataatgcgtc actgctggct gcgagccttt atcttcgcgg gcaaatttat
    20041 cagcgatttt gtccagttct tccaccagtg gataatccag aactgcttct tccatcaacc
    20101 agccgcgcac aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca
    20161 gcagccgtcc ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca
    20221 gacgatgagc ggtaaaaacg tgctggccaa tctgatcacg cacggtttgc atgaccacta
    20281 ctttctcgtc gcgatccagc cagctgcggt gaaccagcca gccggagggt ttatttaccg
    20341 caaccagcca ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag
    20401 agcatccagt ttttccagct cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag
    20461 cgccatttca taatagggtg caacggcaaa agcgcccggt aacggctgtt tgttatctaa
    20521 caaatcgtgc attcgcggga tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc
    20581 cataaagaac ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg
    20641 ctgatgttca cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt
    20701 ggtcataaag caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg
    20761 gagcactgta ttcacagcgc tcccggttcg tttcgcagca ttccagctac ttttgttgct
    20821 ccctgctcat ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta
    20881 ttgcttcctg ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc
    20941 tgacccaccg aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc
    21001 gtcctgcgtc atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt
    21061 gtcctgcaga agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa
    21121 gatagaatcg tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa
    21181 atttcatatg aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat
    21241 tcttatcttt ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat
    21301 ctctcacaac cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc
    21361 cgcaagattt gaggctaaat gcgtccgttt acgtgttccc agcgtctctt tgcacacttc
    21421 accggagaga ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag
    21481 cgtgggcggc agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac
    21541 ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg
    21601 cgcgtgcata tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac
    21661 aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg
    21721 ccagtatacg gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacagttc
    21781 ctcacttaac ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa
    21841 atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat
    21901 acacttgtcg tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca
    21961 tcaggcactt gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc
    22021 cagcctactg caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga
    22081 taacctgcct tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc
    22141 gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct
    22201 gattgagtcg aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa
    22261 ctgggatgag gtgcgccaga cgctggagcg cgacttaagc acttgcgccc agggtaaggt
    22321 tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg
    22381 cacctgcatt gatgaccagg atatcactat cgataactat gaattcacta ctgactatct
    22441 ggagaatgcc accagcggtg aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa
    22501 atcaaactgc ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg
    22561 acgccaaatt gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga
    22621 gttccacgaa cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga
    22681 gaaattaagc gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg
    22741 cagtaatagc gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca
    22801 attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa
    22861 tcaaagggaa tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc
    22921 gcttggctcc atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag
    22981 cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt
    23041 gaccgataac agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg
    23101 ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg
    23161 gcgaattatt cgcgctttgc aggccaactt gttcgcggtc ctgcgtgaca ttctcttcgt
    23221 ttacgggcaa atccataaca ccgttcgttt tcccaacctg aatctcgaca actccgtcca
    23281 catcactaac ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc
    23341 gccaaatatg gtggtctgct ggggcggtca ctcaattaac gaaaacgagt atttgtatgc
    23401 ccgtcgcgtc ggaaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc
    23461 gggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa
    23521 agacagtcgt tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc
    23581 gctggtcaac gaattgatca tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg
    23641 tatcgctcac ggtatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta
    23701 tttgctggga attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac
    23761 cggcccgaaa gagagcgccg actacttccg cgtactggac gagtttgtcg tgcatacgct
    23821 gggtgaaaac gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg
    23881 tcagatgaaa aaatcgatgc cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta
    23941 cagctttaac tggtcaatgc gcattgcgcc agatttgcaa atgccgtttg agccgtctca
    24001 cgagaatatg gctaatctga agctttaccc ggatcaaccc gttgaagtgc tagctgccga
    24061 cctgcgccgt gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc
    24121 cattgaagag tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga
    24181 cgacctgcta cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat
    24241 cccttgctac gaaatctgca cgtaatctcc cctcccccgg tgacgtttgt tgccgggttt
    24301 tctcgttttt ggtcacttac tcatcaactc atttcatttg ttatatgaat gtttcttacc
    24361 accctcacgc gacaaatatc atcacagtta atatgtcata caatttatgt tgcaacgcaa
    24421 acgtttccct attttcataa accgtatttt atcgctatga aaaagaaatt atcgccatga
    24481 ttaactaaaa gtattgattt tttcagttca acctacatat attgcgcgcc ccggaagaag
    24541 tcagatgtcg tttaatgggc aaatattgcc cttaaattct cttttacttt tgatttacag
    24601 agtaaagcgt tgggataatc tatcttccaa gtagattatt gtatttgaga tcaagatcac
    24661 tgatagatac ataacttgtg tgtatctttc cgccctcaaa ttattacggc ggtaaatgat
    24721 taagccatcg ccgatagaca gatttcattt ttacggtcag gcaccttccc gggctgaact
    24781 ggctaaaagc tgaattattt gcattcctcc aggagaaata gatggaaacg actcaaacca
    24841 gcacgattgc gtcgaaagac tctcgtagtg cctggcgcaa gacagacacc atgtggatgc
    24901 tgggccttta cggcacggca atcggcgcgg gcgtgctgtt cctgccaatc aacgccggtg
    24961 ttggcggtat gatcccgctg atcatcatgg ctatccttgc gttcccgatg acattttttg
    25021 cacaccgcgg cctgactcgc ttcgtactgt ctggtaaaaa cccgggcgaa gacatcaccg
    25081 aggttgtaga agaacacttt ggtattggcg caggtaaact gattaccctg ctctatttct
    25141 tcgctatcta cccgatcctg ctggtttata gcgtagcaat caccaataca gttgaaagct
    25201 tcatgtctca ccagctgggt atgacaccac cgccgcgtgc gattctgtcg ctgatcctga
    25261 tcgtgggtat gatgaccatc gttcgcttcg gtgagcagat gatcgttaaa gcgatgagta
    25321 ttctggtatt cccgtttgtt ggcgtactga tgctgctggc tctgtacctg atcccgcagt
    25381 ggaacggcgc agcactggaa acgctgtctc tggacactgc atctgcaacc gggaacggtt
    25441 tgtggatgac cctgtggctg gcaattccgg taatggtgtt ctcgttcaac cactctccga
    25501 tcatctcttc tttcgccgtt gcgaagcgtg aagagtacgg cgatatggca gaacagaaat
    25561 gctcgaagat ccttgcattc gcacacatca tgatggtgct gaccgtaatg ttcttcgtct
    25621 tcagctgcgt actgagcctg actccggcag acctggctgc ggctaaagag cagaacatct
    25681 cgattctgtc ttacctggct aaccacttta acgcaccggt tatcgcgtgg atggctccga
    25741 ttatcgcgat tatcgctatc accaaatcct tcctcggcca ctacctgggc gcacgtgaag
    25801 gcttcaacgg tatggtgatt aaatctctgc gtggtaaagg taagtctatc gaaatcaaca
    25861 agctgaaccg tatcactgcg ctgttcatgc tggtaacgac ctggattgtt gccaccctga
    25921 acccgagcat cctgggtatg attgaaaccc tgggcggccc aatcatcgcg atgatcctgt
    25981 tcctgatgcc gatgtacgca attcagaaag taccggcaat gcgtaagtac agcggtcaca
    26041 tcagcaacgt attcgttgtc gtgatgggtc tgattgcaat ctccgcaatc ttctactctc
    26101 tgttcagcta agtcctttcg cgccgctttc gggcggcgct tcctccgttt taacgcgatg
    26161 tatttcctat gattagcgta ttcgatattt tcaaaatcgg cattggccct tccagttctc
    26221 ataccgttgg accaatgaaa gcgggtaaac aatttaccga cgatctgatt gcccgtaacc
    26281 tgcttaaaga cgtgacccgc gtggtggttg acgtgtacgg ctcgctctct ctgaccggta
    26341 aaggccacca cactgatatc gccattatta tgggcctggc gggtaacctg ccggataccg
    26401 tggatatcga ttccatcccc ggttttattc aggatgtgaa tactcatggt cgcctgatgc
    26461 tggcaaacgg tcagcatgaa gtggagttcc cggttgatca gtgcatgaac ttccatgccg
    26521 acaacctttc tctgcatgaa aacggtatgc gcattaccgc actggcgggc gataaagtca
    26581 tttacagcca gacttactac tctatcggcg gtggctttat cgttgatgaa gaacattttg
    26641 gccagcagga tagcgcaccg gttgaagttc cttatccgta cagttcagca gccgatctgc
    26701 aaaaacattg tcaggaaacc gggctgtcac tctctggcct gatgatgaaa aacgagctgg
    26761 cgctgcacag caaagaagag ctggaacagc acctggcgaa cgtctgggaa gtcatgcgcg
    26821 gcggtattga gcgcggtatt tctaccgaag gcgtgttgcc tggcaaactg cgcgtaccac
    26881 gccgtgctgc ggcactacgc cggatgctgg tcagccagga taaaaccacc actgacccga
    26941 tggcggttgt tgactggatc aacatgtttg cactggcagt gaacgaagag aacgccgctg
    27001 gcggtcgcgt ggtgactgcg ccgactaacg gtgcgtgcgg gattatcccg gctgtgctgg
    27061 cgtactacga taagtttatc cgcgaagtga acgctaactc actggctcgt tacctgctgg
    27121 tagccagtgc cattggttct ctttataaga tgaacgcgtc aatttctggt gctgaagtcg
    27181 gctgccaggg tgaagttggt gtggcgtgct caatggcggc ggctggtctg gcagaactgt
    27241 taggtgcaag cccggcgcag gtgtgcatcg cggcggaaat cgccatggag cacaacctcg
    27301 gtctgacgtg tgacccggtc gccggacagg tccaggtgcc atgcatcgag cgtaacgcca
    27361 ttgcggcagt aaaagcggtg aacgccgcac gtatggcgct acgccgtacc agcgagccgc
    27421 gcgtctgcct cgataaagtt atcgaaacca tgtacgaaac aggtaaagat atgaacgcca
    27481 agtaccgcga aacctctcgc ggcggcctgg caatgaagat cgttgcctgc gattaatcgc
    27541 tctccagagg cctcgttttg cgaggcctct tcccgatttc tcatccagcc gtagcctgtt
    27601 ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg gctgttcatt
    27661 tgcttattgt cgatgcactg aatcttattc gtcgcattca tgccgttcag ggatcgccct
    27721 gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag ccaacccacg
    27781 cggtcgccgt ttttgatgat gaaaaccgca atagcggctg gcgtcatcag cgtttaccag
    27841 attacaaagc gggtcgaccg ccaatgccgg aagagttgca cgacgagatg cctgcattac
    27901 gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcaac cagcggcaac gaagccgatg
    27961 acttagccgc cacgctggcg gtcaaagtga cacaggccgg gcatcaggca acgattgttt
    28021 cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt gattacttcc
    28081 agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt
    28141 tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa
    28201 tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa gggatatatg
    28261 agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg
    28321 cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac ggcaatttac
    28381 agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaacgtc gtatccggca
    28441 ttatcacatc agcgcattta ccaggcggta tggtaaagct ctacaatatc ctcaagcgtt
    28501 gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg ccgcctgcgc cagtgccgga
    28561 atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg gaataccgac atcacggttg
    28621 agagcaaaca ccgcttcaac agcggcatta cgcgcctctt ccaggctcat accttccact
    28681 ttcacgccca taacgcgcgc gatatcgcgg tacttctcac cggtaaagtc agcgttatag
    28741 cgcatgacat gcggtaacag gatggcgttc gcaacaccgt gtggagtgtt gtaaaacgcg
    28801 cccagtggat gcgccatacc atgcaccaac cctaacccaa cattcgagaa gcccataccc
    28861 gcaacatact gcccgagcgc catttcttct ccggcatcct tatcaccagc aaccgatcct
    28921 cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca gtgcatcggt tagtgcccac
    28981 gcgccacggg taatataccc ctcaatagca tgagtgagcg catcgacacc cgtcgcggct
    29041 ttcagcgctg gaggcatacc atccatcatg tcagcgtcaa taaacgccac ctgcgggata
    29101 tcatgcggat caacgcaaac aaacttgcgc cgtttctctt cgtcagtgat cacgtagtta
    29161 atggtcactt ctgccgcagt acctgctgtg gtaggaattg ccagaatcgg tacactgggt
    29221 ttattggtcg gggaaagccc ttccaggcta cgcacatcgg caaactccgg gttgttgctg
    29281 ataatgccaa tcgctttaca ggtatcctgt ggagaaccac caccaatagc gatcaggtaa
    29341 tccgcgccgc tattctggaa tacaccgagc ccttctttga cgacagtaat tgttgggttg
    29401 ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag catccatctt atcggtcact
    29461 ttcgccacca cgccgcattg caccagcgtt ttatcggtga cgatcagcgc cttctgataa
    29521 ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac cccgaccaaa ccatgccgtt
    29581 tcgttcagaa tcattctgtt agccatcatc cttctccttg ttgctttacg aaattactct
    29641 tcaatccgta acccataggt tttgaatttc tccagcacta cggcaatctc ttcatcgctc
    29701 agcactggca ccgggtccgt aatcgccagg gtcgtcaggt aaagttgcgc cagcacttca
    29761 acttcatgcg ccagccataa cgctttttcc agattcacct cacaagcgat aagcccatga
    29821 tgttgtaaca aagttgcctt acgatttttg agagccagcg caacatgttc agaaagttcg
    29881 cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat taccacctgc cgccgcaatc
    29941 atgtagtgaa tagcggggat cggtcggtta agaatggaaa ctgctgtgca atgaacggca
    30001 tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat aggccgccat atggaaacgc
    30061 cattcgcttg aggggagctt tccttcctca tgtttaccgt tgccatcaat aaagacaata
    30121 tgcgactccg tcagtttttc atatggaatg cctgtaggcg taatcagcat cccatcctga
    30181 taacgtacac tgacgttccc cgctgtcccc tggttcagtc ccaggcgggt catttccagg
    30241 caagtgtcaa taatctgacg agcaagttta tttcgttcca ttagctacct ctctctgatt
    30301 caaaacaggg caataatgtt gttcctttca cactattgaa ttagccgttt aattacccac
    30361 catcttcttc ctgattaaca agaaagaaat tcacaagctt atattttgtg acctggttca
    30421 actaatcaca gtaaataact gcaagttctc tttttataac cccattaaaa atgaccgctc
    30481 ttaaaaatat ttatcaaaac ggtcattttt ctattcaccc aagccaggaa tgcccgtttt
    30541 cggcataaac aattaatacg gtcatctggt ttgtgttttt tatgatttat tttctgaaac
    30601 gggcatgaaa tttcgattat taaagtgatg gtagtcacat aaagtcacct tccagctaat
    30661 aagtgtgacc gccgtcatat tacagagcgt tttttatttg aaaatgaatc catgagttca
    30721 tttcagacag gcaaatattc actgatatga agcccgaact cgctggtttt gcacttttga
    30781 aaacataacc gattacgtgc ttaagcttct gaacctaaga ggatgctatg ggaaacacat
    30841 caatacaaac gcagagttac cgtgcggtag ataaagatgc agggcaaagc agaagttaca
    30901 ttattccatt cgcgctgctg tgctcactgt tttttctttg ggcggtagcc aataacctta
    30961 acgacatttt attacctcaa ttccagcagg cttttacgct gacaaatttc caggctggcc
    31021 tgatccaatc ggccttttac tttggttatt tcattatccc aatccctgct gggatattga
    31081 tgaaaaaact cagctataaa gcagggatta ttaccggatt atttttatat gccttgggcg
    31141 ctgcattatt ctggcccgct gcagaaataa tgaactacac cttattttta gttggcctat
    31201 ttattattgc agccggatta ggttgtctgg aaactgccgc aaaccctttt gttacggtat
    31261 tagggccaga aagcagtggt cacttccgct taaatcttgc gcaaacattt aactcgtttg
    31321 gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt gtctaacgtg ccacatcaat
    31381 cgcaagacgt tctcgataaa atgtctccag agcaattgag tgcgtataaa cacagcctgg
    31441 tattatcggt acagacacct tatatgatca tcgtggctat cgtgttactg gtcgccctgc
    31501 tgatcatgct gacgaaattc ccggcattgc agagtgataa tcacagtgac gccaaacaag
    31561 gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg tcactggcgc tgggcggtat
    31621 tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg gagctatttg attcgctacg
    31681 ctgtagaaga aattccaggt atgactgccg gctttgccgc taactattta accggaacca
    31741 tggtgtgctt ctttattggt cgtttcaccg gtacctggct catcagtcgc ttcgcaccac
    31801 acaaagtcct ggccgcctac gcattaatcg ctatggcact gtgcctgatc tcagccttcg
    31861 ctggcggtca tgtgggctta atagccctga ctttatgcag cgcctttatg tcgattcagt
    31921 acccaacaat cttctcgctg ggcattaaga atctcggcca ggacaccaaa tacggttcgt
    31981 ccttcatcgt tatgaccatt attggcggcg gtattgtcac tccggtcatg ggttttgtca
    32041 gtgacgcggc gggcaacatc cccactgctg aactgatccc cgcactctgc ttcgcggtca
    32101 tctttatctt tgcccgtttc cgttctcaaa cggcaactaa ctgaacatat tttccgaata
    32161 aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa attggtatcc gcccggttat
    32221 tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa caaacaatga atatggcgaa
    32281 agctacggcc gcactgctga ccgagaaact gcgccatgcc tgcggagctg ccgtcgagtg
    32341 tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc gctgcttgcg aagaaaaatt
    32401 cagcagccag aatgtaggcc tcaccattac ggtaacgcct tgctggtgct atggcagtga
    32461 aaccatcgac atggatccaa cccgcccgaa agccatttgg ggctttaacg gcactgaacg
    32521 tcccggcgct gtttacctgg cagcggctct ggcagctcac agccagaaag gcatcccggc
    32581 attctccatt tacggtcatg acgttcagga tgccgatgac acatcgattc ctgccgatgt
    32641 tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc gtcgccagca tgaaaggtaa
    32701 aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc ggttctattg ttgatcacaa
    32761 cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg gatatgaccg aactgcgtcg
    32821 ccgtatcgat cagaagattt acgacgaagc cgaactggaa atggcactgg cctgggctga
    32881 taaaaacttc cgctatggcg aagatgaaaa taacaaacag tatcaacgca atgccgagca
    32941 aagccgcgca gttctgcgcg aaagtttact gatggcgatg tgtatccgcg acatgatgca
    33001 aggcaacagc aaactggccg atattggtcg cgtggaagaa tcacttggct acaacgccat
    33061 cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa tatcccaatg gtgacaccgc
    33121 cgaagcgatc ctcaacagtt catttgactg gaatggcgtg cgcgaaccct ttgtcgtggc
    33181 gaccgaaaac gacagtctta acggcgtggc aatgctaatg ggtcaccagc tcaccggcac
    33241 cgctcaggta tttgccgatg tgcgtaccta ctggtcacca gaagcaattg agcgtgtaac
    33301 ggggcataaa ctcgatggac tggcagaaca cggcatcatc catttgatca actcaggttc
    33361 tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa ggtaacccga cgatgaagcc
    33421 gcactgggaa atctctcagc aagaggctga cgcttgcctc gccgctaccg aatggtgccc
    33481 ggcgatccac gaatacttcc gtggcggcgg ttactcttcc cgcttcctta ccgaaggcgg
    33541 cgtcccgttc accatgactc gtgtcaacat catcaaaggc ctgggaccgg tactgcaaat
    33601 cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat gacatcctca acaaacgcac
    33661 caactcaacc tggccaacca cctggtttgc accgcgcctc accggtaaag ggccgtttac
    33721 ggatgtgtat tcggtaatgg cgaactgggg cgctaaccac ggggttctga ccatcggcca
    33781 cgttggcgca gactttatca ctctcgcctc catgctgcgt atcccggtat gtatgcacaa
    33841 cgttgaagag accaaagtat atcgtccttc tgcctgggcc gcgcacggca tggatattga
    33901 aggtcaggat taccgcgctt gccagaacta cggcccgttg tacaagcgtt aatacattac
    33961 tccctgatgt gatgcccggt cgctccggct accgggcctg aacaagcaag agtggttagc
    34021 cggataagca atgttatccg gctatattgc aggagcgatt atgaaacaag aagttatcct
    34081 ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg gttaatcggc agggaaaaat
    34141 tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg atggaaaaca acacctggca
    34201 ccagtggtct ttagacgcca ttttgcaacg ctttgctgat tgctgtcggc aaatcaatag
    34261 tgaactgact gaatgccaca tccgcggtat cgccgtcacc acctttggtg tggatggcgc
    34321 tctggtagat aagcaaggca atctgctcta tccgatcatt agctggaaat gtccgcgaac
    34381 agcagcggtt atggacaata ttgaacagtt gatctccgca cagcggttgc aggctatttc
    34441 tggcgtcgga gcctttagtt tcaatacgtt atataagttg gtgtggttga aagaaaatca
    34501 tccacaactg ctggaacgcg cgcacgcctg gctctttatt tcgtcgctga ttaaccaccg
    34561 tttaaccggc gaattcacta ctgatatcac gatggccgga accagccaga tgctggatat
    34621 ccagcaacgc gatttcagtc cgcaaatttt acaagccacc ggtattccac gccgactctt
    34681 ccctcgtctg gtggaagcgg gtgaacagat tggtacgcta cagaacagcg ccgcagcaat
    34741 gctcggctta cccgttggca taccggtgat ttccgcaggt cacgataccc agttcgccct
    34801 ttttggcgct ggtgccgacc aaaatgaacc cgtgctctct tccggtacat gggaaatttt
    34861 aatggtccgt agcgcccagg ttgatacttc gctgttaagt cagtacgccg gttccacctg
    34921 cgaactggat agccaggcag ggttgtataa cccaggtatg caatggctgg catccggcgt
    34981 gctggaatgg gtgagaaaac tgttctggac ggctgaaaca ccctggcaaa tgttgattga
    35041 agaagctcgt ctgatcgcgc ctggcgcgga tggcgtaaaa atgcagtgtg atttattgtc
    35101 gtgtcagaac gctggctggc aaggagtgac gcttaatacc acgcgggggc atttctatcg
    35161 cgcggcgctg gaagggttaa ctgcgcaatt acagcgcaat ctacagatgc tggaaaaaat
    35221 cgggcacttt aaggcctctg aattattgtt agtcggtgga ggaagtcgca acacattgtg
    35281 gaatcagatt aaagccaata tgcttgatat tccggtaaaa gttctcgacg acgccgaaac
    35341 gaccgtcgca ggagctgcgc tgttcggttg gtatggcgta ggggaattta acagcccgga
    35401 agaagcccgc gcacagattc attatcagta ccgttatttc tacccgcaaa ctgaacctga
    35461 atttatagag gaagtgtgaa atgctgaaaa caatttcgcc gttaatttct cccgaactat
    35521 tgaaagtgct ggcagagatg ggacatggag atgaaattat tttttccgat gctcactttc
    35581 ccgcccattc gatgggaccg caggtgatcc gcgctgatgg cctgttggtg agcgacttgc
    35641 tccaggcgat tatcccgtta tttgaactgg acagttatgc accgccgctg gtgatgatgg
    35701 cggcggtaga aggtgacact ctcgatcctg aagtagaacg acgttaccgt aatgcgcttt
    35761 cactacaagc cccgtgtcct gacatcatcc gcatcaatcg ttttgcgttt tatgaacggg
    35821 cgcaaaaagc ctttgcgatc gttatcacag gcgaacgagc gaagtacggg aatattcttt
    35881 taaaaaaagg ggtaacaccg taatctcata ccggtacgcc cgcatgacgc gggcggttat
    35941 cgaatgatgg ggtgaaaaat atgaaagcgg cacgccagca agcgatagtc gacctgctgc
    36001 tgaaccatac cagcctgacc acggaagctc tctctgaaca gctaaaggtc agtaaagaaa
    36061 ccattcgtcg cgatctcaat gaattacaga cgcagggtaa aattctgcgc aatcatggac
    36121 gcgctaaata tatccaccgt caaaatcaag acagtggcga tccctttcac atcaggctga
    36181 aaagccatta tgcgcataaa gcagatatcg cgcgcgaggc gctcgcgtgg attgaagaag
    36241 ggatggtgat agccttagac gccagttcaa cttgctggta tctggcacgc cagttgcctg
    36301 acatcaacat tcaggtcttc accaatagcc atccgatttg ccatgaactc ggtaaacgcg
    36361 aacgcattca actgatcagt tccggcggca cacttgagcg caaatatggc tgttacgtca
    36421 atccctcgct gatttcccaa cttaaatcgc tggaaatcga tctgtttatt ttttcttgtg
    36481 aagggatcga tagcagcggc gcactgtggg actccaatgc gatcaacgct gattacaaat
    36541 cgatgctatt aaaacgtgcc gcgcaatcgt tgttattgat tgataaaagt aaatttaatc
    36601 gttcagggga agcccgcatc gggcatctgg atgaggtaac gcacattatt tctgatgagc
    36661 gccaggttgc aacttctttg gtaacagcct gacggaaagg gtagcaggcc ggagacgacg
    36721 ccccggcctt gcctgttatc gctcgtcgcg acgaccaccc accgcagccc agatgcggcg
    36781 gacgtgcacc gtcacttctt cgcgatcgtg atacaactgc cgcgcctgaa tctgagcatt
    36841 tatgccatgt tcatcaagct gtgcctgaat atacgccaga ttgtgtgaca cttcttcgta
    36901 gcgttttttc atcggcagtt tgaggttgaa aatggtttca cggcaccagc cattaaccag
    36961 ccactgcgcc atcaatgccg caactttcgc cggtttttca accatatcgc ataccatcca
    37021 ggagatattg ctgcgcgtcg gacggaattt gaaaccgtct tcccgcagcc acgtcacctg
    37081 tccggtatcc atcagacttt gcgccatcgg gccgttgtcg acggaataaa cccacatgtt
    37141 gcgcttcacc agttggtagg tccagccgcc agggcaagcg cctaaatcca ccgcccacat
    37201 cccgttcgcc aggcgttcat cccactcatc cgcaggaata aacacatgaa atgcctcttc
    37261 cagtttgagc gtggaacgac tcggcgcatc tgccggaaat ttcaggcgcg gaatgcccat
    37321 atagaacggc gaattattgt tgctgtatga gtaaccggta tagcagcagc ctggtgcaat
    37381 aaagaataca tgcacaaccg gacgcttcgg cgtttcatag ttcgccagca ccccggcatc
    37441 gcgcaaggca gcgcgtagcg gaacagtaaa tttacggcag aatttcagta actctttgct
    37501 ttcgttggta tcggcaactt caacacgcag ttcaccgccc ttctctacta cgccctgtaa
    37561 catgccgaca atgggggtaa tacgatcttc tggcggcaaa tgctgcagga gttcccccac
    37621 cacaaaccac tggcgggcaa aaattaatga actgaacggc agctcacgga ttaacttatc
    37681 gccatcatca ggttgataac attcataaat gacataaccc gcattctctt tcacgcgggc
    37741 aaaaccgaaa atttcccgct ggccggcttt atcggtaatt tctgcggcgc actctttttc
    37801 aaagcccgga cggcacagca atacaacctt attcatgact tacgcccttg cgctttaaac
    37861 ggatagcacc aactaacatt aacgcccagc ccgcgaggaa gctcacgccg ccaaccggag
    37921 tgacaaacgc ccacaaacgc agatgggaca gcgccaggca ataaaggctg ccgctgaaca
    37981 acaccgtgcc taacgcgagg aaaacgctac tccagtaaaa ccagatactg atgcgacgct
    38041 gcattgccac cgccagacct aagatcgcca gcgtatgaaa cgcctggtat tcgaggccgg
    38101 tctggatcca gcccatctca acggccccca tggttttact taacacatgc gcgccaaaag
    38161 cgcccagagc cacaaaaatg aagccgctaa tggcggcgaa aatcagcata aaacggctgg
    38221 tcatggtcgt accctacgta aattattgtt cataacgaaa gcggaatttt tcttgttcag
    38281 cagcagcttt cgccaggatc cattgacgaa aggcggctat tttacccagt tctgcctggc
    38341 tgtcatgaca aaccagataa aaagcgtttt tactgaccag aacatcatta aacgggcaaa
    38401 caagacgtcc ggcctcgatt tcagattgcg ccatcacgtt atttgccagc gccactccct
    38461 gcccgtgaat agccgcttgc agcaccatgg cgctatggct aaaaattggc ccttgctgaa
    38521 cgttgatatg atttaacccc aattgtcggg tatatgtctg ccagtcacgg cgtgacgcat
    38581 catgtaataa cgtatgttta gccagatctt ccggggtctt caagggtttt tcgccagtca
    38641 gcagtagcgg cgaacacacc ggcaataaat attcggcgta aagtttttcc acccgtagcc
    38701 ccggccagtt gccccgacca taaaatatcg ccacatcaac atcatccgcc agcttatctt
    38761 cctgacgatc aaccgcctgg attcgaacgt caattcccgg ataagctgaa ttaaagctgg
    38821 aaagtcgcgg aaccaaccaa tgaatggcga aactggggag taaactgacc gtcaacgccc
    38881 ccttggcgct acgggcctgg agtttacgcg tcgcttcggt taattgcgaa aatatctctt
    38941 tgatatcgag gaaatagctt tgcccttcct cggtcagcag gagtgaacga ttacggcggc
    39001 ggaacagttt tagccccaaa aaatcctcaa gagacttgat ttgatgactt actgcggcct
    39061 gggtcacaaa aagctcttct gctgcgcgag tgaaacttaa atggcgtgct gcggcatcaa
    39121 aaactcgtaa ggcatttagc ggtggtaatc gtttagacat ggcttttaaa ctttgatgtt
    39181 aaatgaattt aacaattaga tcacactatg taacctatta gtttttttaa tctgagccat
    39241 tataaattgt ccgttgagct tctaccagca aatacctata gtggcggcac ttcctgagcc
    39301 ggaacgaaaa gttttatcgg aatgcgtgtt ctggtgaact tttggcttac ggttgtgatg
    39361 ttgtgttgtt gtgtttgcaa ttggtctgcg attcagacca tggtagcaaa gctacctttt
    39421 ttcacttcct gtacatttac cctgtctgtc catagtgatt aatgtagcac cgcctaattg
    39481 cggtgctttt ttttaccttg cgatcgcgaa ttactgatcc agttcgacca tctctttcac
    39541 gtcagtacgg ttgatctgct gtttgttgcc attagcgtct ttatacgaaa tcataccggt
    39601 atcgttatca gtctgtggtt tgccgtcaga gacgatggta cgtccgtcat tggtgtgcat
    39661 cacatagttc gaaccggaac aggcgctcag ggcaaaagtc agcatacagg cagaaataat
    39721 tgcggcagtc tttttcatca tcttctcctt aaagcgatta ataaatctta atagtattga
    39781 ttaacaggct aaaattaacg cctaacacta ttcagcatat gttacttacg cggcttcgcc
    39841 aggatatcca gataattctg atggttagca ctctccttgt atcaaagtga attttgcgtc
    39901 acgatcggtg catcaagccg aggagtacca tgaacgtttt taatcccgcg cagtttcgcg
    39961 cccagtttcc cgctctacag gatgcgggcg tctatctcga cagcgccgcg accgcgctta
    40021 aacctgaagc cgtggttgaa gccacccgac agttttacag cctgagcgcc ggaaacgtcc
    40081 atcgcagcca gtttgccgaa gcccaacgcc tgaccgcgcg ttatgaagct gcacgagaga
    40141 aagtggcgca attactgaat gcaccggatg ataaaactat cgtctggacg cgcggcacca
    40201 ctgaatccat caacatggtt gcacaatgct atgcgcgtcc gcgtctgcaa ccgggcgatg
    40261 agattattgt cagcgtggca gaacaccacg ccaacctcgt cccctggctg atggtcgccc
    40321 aacaaactgg agccaaagtg gtgaaattgc cgcttaatgc gcagcgactg ccggatgtcg
    40381 atttgttgcc agaactgatt actccccgta gtcggattct ggcgttgggt cagatgtcga
    40441 acgttactgg cggttgcccg gatctggcgc gagcgattac ctttgctcat tcagccggga
    40501 tggtggtgat ggttgatggt gctcaggggg cggtgcattt ccccgcggat gttcagcaac
    40561 tgaatattga tttctatgct ttttcaggtc acaaactgta tggcccgaca ggtatcggcg
    40621 tgctgtatgg caaatcagaa ctgctggaag cgatgtcgcc ctggctgggc ggcggcaaaa
    40681 tggttcacga agtgagtttt gacggcttca cgactcaatc tgcgccgtgg aaactggaag
    40741 caggaacgcc aaatgtggct ggtgtcatag gattaagcgc ggcgctggaa tggctggcag
    40801 attacgatat caaccaggcc gaaaactgga gccgtagctt agcaacactg gcggaagatg
    40861 cgctggcgaa acgtccaggc ttccgttcat tccgctgcca ggattccagc ctgctggcct
    40921 ttgattttgc tggcgttcat cacagcgata tggtgacgct gctggcggag tacggtattg
    40981 ccttgcgggc cgggcaacat tgcgctcagc cgctactggc agaattaggc gtaaccggca
    41041 cactgcgcgc ctcttttgcg ccatataata caaagagtga tgtggatgcg ctggtgaatg
    41101 ccgttgaccg cgcgctggaa ttattggtgg attaatgacg aacccgcaat tcgccggaca
    41161 tccgttcggc acaaccgtaa ccgcagaaac gttacgcaat accttcgcac cgttgacgca
    41221 atgggaagat aaatatcgcc agttgatcat gctggggaaa cagcttccgg ctttgccaga
    41281 cgagttaaaa gcgcaggcta aagagattgc cggatgcgaa aaccgcgtct ggctgggata
    41341 taccgtggct gaaaacggta aaatgcactt ctttggcgac agcgaaggac gtatagtgcg
    41401 cggcctgctg gcggtgctgt tgactgccgt tgaggggaaa accgccgccg aattgcaggc
    41461 acagtcgccg ctggcattgt ttgatgagct gggattgcgt gggcagctta gcgcctcacg
    41521 cagccagggg ttgaatgcgt taagcgaggc gattatcgct gcgacgaagc aggtttaagc
    41581 acagaaccgg gtcggataag acgttcgcgt ctcatccgac ctgattgttt aaccctgacg
    41641 cgccgctttc gccatcatct tcttcagcgc atgagaaacc gcaacaaacc caaaggtggc
    41701 ggtcaccatc gttgccgcgc caaatccaga tgcacaatcc atccgcttcg gcccttctgc
    41761 cgtggctttc atcgcacaca cagtaccgtc tgactgcggg tacaccagcg cttcagtaga
    41821 aaacacgcaa tcgacgccga gcttaccttt actgtttttc actacgccaa aatcgctttt
    41881 caggcgctcg cgcaacttcg ccgccagcgg atcctgaatc gttttcgcca gatcggtaac
    41941 ctgaatctgc gtcgggtcaa tctgcccacc cgcgccaccg gtcgtgacca gcgggatttt
    42001 attgcgccga caataagcaa tcagcgccgc tttgggccgt acactatcaa tggcatcaat
    42061 cacgtacgaa taacccacgc tcatatactg cgctacgtta tccggcgtca cgaaatcatc
    42121 caccaccgtt acacggcact ccgggttaat ctggcgaata cgctccgcca tgacttccgc
    42181 ttttgccagc ccgacgttat cgcgcagagc gtgaatttgc cgattggtat tggtgacgca
    42241 cacatcatcc atatcgataa gcgtgattgc gccaatcccc gtgcgcgcca gcgcttccgc
    42301 tgcccaggaa ccgacaccac cgataccaac cacacaaata tgcgcgtcag caaacagttg
    42361 caacgctttt tcaccataca gacgcgctgt gccaccaaaa cgctgacgcc atgcatcact
    42421 aattaccaca gacatagcac ctcagaatta aaaagggtga ggttatccct caccctgtcg
    42481 tatccgtaaa aacggtatac agaataacac atcagccgct aaagacgtta cctgcgcccg
    42541 gggcggtttt cagcacccag acacgtccat agtggttgta ccaacctgcg cgatgtccgg
    42601 cttccggccc gatcccttga tagatatcga agtgttggcc tttgattgcg ccaccgacat
    42661 ccagcgccac catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca
    42721 gcggcacttc tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag
    42781 aagcacggcc aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt
    42841 taaagaaaac gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg
    42901 tttcgcccca atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt
    42961 cgatcagcac cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa
    43021 agttaagcgg actgccatca ccaaagtcga tatacccgct accctgcaca tccataatga
    43081 agttatccat cagggagttg ctgtaagcga gaatatattt atcactcagc gcccctgcgt
    43141 agatctccgc acgagacggc agacgaccac gttttggcgg catacggtaa atcggatact
    43201 ggaactcgcc ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact
    43261 gcacgttacc atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca
    43321 tattgcgggt atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct
    43381 ggttgccata cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac
    43441 cggcgttaat cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa
    43501 atttcccgtc tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca
    43561 ttgccacaac cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct
    43621 tcaggttaaa aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca
    43681 gcctctccct gacgcaaatt ttgcacaaaa aataggcttt agtgatttgt ttttgttcaa
    43741 aatcatgcca aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta
    43801 tagtgcgcat ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg
    43861 aaggtcgtcg gttcaaatcc ggcccccgca accaattaaa atttgatgaa gtaaagcagt
    43921 acggtgacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    43981 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcaat acggtgacgc
    44041 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    44101 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gctctcctaa
    44161 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtc gccccatcgg
    44221 caaaatacgc tttaattccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    44281 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    44341 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    44401 tgttgatttt accgagctta ttcagtaccg ctttaccaaa cttcaggctg tcggcaatgg
    44461 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    44521 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    44581 ttgcaccttt ggttgagagc gcaaacacag aggaaccgct cggctgacga ctggtaaagg
    44641 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    44701 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    44761 tctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacgtctttt tcgcgcgttt
    44821 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    44881 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgtgctggc ggcacctgct
    44941 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    45001 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    45061 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatccgt acggtctgcg
    45121 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    45181 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttccg
    45241 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacacggg
    45301 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctgaccgca gccagactga
    45361 cctgacttac gctcaatagc cacatggcac ccgcgccttg cagtaaacga cgacggctga
    45421 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    45481 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    45541 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    45601 tttacacttg caccctgcga aaaaacagaa taaaaataca ctaatttcga ataatcatgc
    45661 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    45721 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    45781 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    45841 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggccgcgca
    45901 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    45961 ggtgaagcag gctgcgggaa cattgcaact ggacatcact gctcgcctgt cgatgagtct
    46021 caataacacg ccgctacagg gcgcgcatat caacgtcgtc agtggcaatt ttattattgc
    46081 ccagccgctg ggcgtcgatg acggggtgga ttactgccat agcgggcgta tccggcggat
    46141 tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa tggggccggt
    46201 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    46261 ggccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccaag gcgtcactaa
    46321 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    46381 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    46441 agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag atggcgcgct
    46501 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    46561 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    46621 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    46681 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    46741 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    46801 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    46861 attatttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccagt
    46921 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    46981 agtgttgatg gcggatttag ggtaagcgat gaaaatcgtc ggatgcgaca tgcgtaacac
    47041 tcgtacgtcg catccggcaa ttacgtttat tcccgtgaac taaacaacgc cgccagacca
    47101 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tgcgctcatc ggcatacagc
    47161 gacagacggc gacgcgcgcg ggtcaccgcg gtataaacca gctctcgcgt tactaccggc
    47221 gtgcgttggc tcggcaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    47281 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    47341 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    47401 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcgacct
    47461 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgctca
    47521 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    47581 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    47641 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    47701 aaaagccgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    47761 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    47821 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    47881 ggaacgtgag tccccgtcag gcggcttagc tgcccggcac gctcggcggt aaagccggca
    47941 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    48001 cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag tctcgacatc
    48061 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    48121 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg gtgcaaagta
    48181 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    48241 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    48301 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gtaacttcgc cacggtggtc
    48361 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    48421 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    48481 gccagtagag cttcatcaac ctcaatggca tgattcacct cattgaagaa gcgcgctacc
    48541 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    48601 gtgggttcat ctcccctgct gactgcttga gaagccagca agcattcttc ccaattttgt
    48661 agctcaccga tttcactgac acaggtcgcc aacagcggat gcgacgcctc gttattttcc
    48721 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    48781 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    48841 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    48901 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    48961 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    49021 taacgccgcc aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    49081 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    49141 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    49201 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    49261 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    49321 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    49381 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agagatgcct
    49441 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    49501 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcc aggtcttcaa
    49561 acaaactgtg caagaacgtc cccggtgacg caccgcgcgg aaactgatgt ggtgttaacg
    49621 tcggttcttc aacgacgctg gcaacgcctg cagcatcgac atccagccga ggcatcaaat
    49681 cctgggcgat accgtgacca cgctgttgca aaccagagta gctggtgacg cgccagttat
    49741 cgccgggcaa tcgttgtaac gtcttcgcat tcagctctgc tgtagaaaca tcattaacct
    49801 gccagggttg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    49861 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    49921 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    49981 gtgcaacgcc gagactgcaa tgccaaaccg aacgtgtcag cgccacgtaa agcaaacgca
    50041 gatcttccgc cagacgttcg gcctccgcga ggtcgacgct ttctggcgca gcattaagat
    50101 ccagaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgctcctgg acgcggaaat
    50161 tggtgataaa cggcagccag accaatggat attccagccc tttcgatttg tggatcgtga
    50221 caatctgcac cagatgttta tcactttcga gacgcatttg ttggctggag gcattactgt
    50281 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    50341 ttccggcttc ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    50401 ctgccgttgc cagcaagttt tcagcaatgt tacgcgccga catcagcgcc cgcagcatcg
    50461 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccacat
    50521 cccacgcatg ttcgtcattg ttcagcgttt cgatatccag cgcgttcagc cccatcattg
    50581 acgttgccag cgcactacgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    50641 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    50701 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    50761 gcaccagcac actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    50821 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    50881 ccatggtact ttgataatcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    50941 tcgcaggctg tgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    51001 tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg ctgaaaagct
    51061 tattcacgct gttcaccatt cctggtgcgg aacgccagtt ggtgtctaaa gtgtagtggg
    51121 cgtgaacttc gctacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    51181 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    51241 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    51301 ggaatcgcgt acggatcgcc gctgccaaca cctcaccgct ttcgctacgc agcgcggaat
    51361 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    51421 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaccag atcgcggatc gacaatggtt
    51481 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggctt
    51541 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactctggc aactgataac
    51601 tgttggtctc ttcttctgcc caggcgctga tcttgtcgat ccatttagcc tgattgctac
    51661 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    51721 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    51781 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cgcttcgcct tgcagataac
    51841 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    51901 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    51961 gcagagactc atcttcaatc agctgctgct caaacagcat accggattca aaggcattca
    52021 ggttgagcat gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca tccatctgcc
    52081 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    52141 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    52201 tgctacggat acgaccacgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    52261 gttcttcaac ggtcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    52321 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    52381 gctcaccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    52441 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    52501 tcagggtgta catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    52561 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    52621 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    52681 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    52741 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    52801 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    52861 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    52921 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    52981 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    53041 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    53101 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    53161 tcgcgtgcca gcgttgttgc ctgggcttcg gtcatgttgc caataaccat aaactctggt
    53221 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    53281 aaaattttgc gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    53341 ataatcgcct gctcgaacgc tttgcccttt tctgcggaat ccatcatctg gttataccag
    53401 gacttcgcct gctcaagctg atcttccgtg gcggtatagc taaagtagcc ttccagcaat
    53461 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaaccat aaggccgttg
    53521 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    53581 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    53641 gctttcggat tacgcaaaat caggctgaca tcggcttttg gctcgctggc aaaataacgg
    53701 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcatat
    53761 ttcttgtctg acttaatcag cgagaaatcg tcaggaatat aagggttaag ctctggcaaa
    53821 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    53881 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    53941 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    54001 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    54061 atggtatctg ccagccattc gacgtaatcc atatcacggg tgattgacgg ataacggaag
    54121 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcgatccc tttttcacgt
    54181 aacaaattga ggtagctaaa aatagccgcc acaacctgat cgcgattcgc cagaccttta
    54241 tcggttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    54301 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcggaaag tgtacctggg
    54361 ctgcgattac caatcagata ggtaatcaat tcatcggttt tgctacggaa tttcgccgag
    54421 ttgttatcaa tacgaaactc tacgcgcaac actttacgcg gcagcgccgg gacgtaatga
    54481 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    54541 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    54601 ttattgctgt aaataaccgc cttcatcaga ttggcggaat agtacttctc gtggaaatct
    54661 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    54721 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    54781 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    54841 gcgtatttct tgtcgagcaa gggttctgca atagcatcag ccaggcggtc taccgcacca
    54901 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    54961 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    55021 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    55081 tcttccagcg acccaacggg caccaccagc gccgagagcg atttaactgc ctgcggatca
    55141 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    55201 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaag
    55261 ggtgcccaaa gggcaactaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    55321 ggacctcata atcaacttaa ttttctgtcc agattcaaca cgttaacgca aatcatctgc
    55381 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    55441 tggtgcgcat cataaagtaa gcggataaat tgcgcaattt ttatacagca ctcatgactg
    55501 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    55561 ctctggtgtt aattgccgcc agagcctttg ataccagata tcatcacctt cgccacgcac
    55621 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    55681 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    55741 ttcaggtaac accagcaatg gcgcggacat cccttcacga tacccctcaa tcagttgtga
    55801 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc cgtctttgcg
    55861 tagaaaaagg cgactttcac cattaccacc gctggcacag tagacaaggt gttccagcca
    55921 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    55981 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    56041 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    56101 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    56161 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    56221 gatttgataa cggctaagcc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    56281 ttcagtacgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    56341 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    56401 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    56461 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    56521 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    56581 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgctc
    56641 actgttatcc tgaatggaac gaccgatata gctgatatag agtttttgct gcgcggaaat
    56701 taacgcttcc aggaacagat agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    56761 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    56821 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    56881 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    56941 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    57001 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    57061 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcca gcggacgctc
    57121 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agggaagcca gatgccccac
    57181 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    57241 cgcgctctcc atcgcgtaac ccagcaacat gcgcgtcagg ccaaaccgcc aggtgtgttg
    57301 accggtagcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    57361 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    57421 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    57481 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    57541 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacacag cctgaataaa
    57601 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    57661 gtcttcttcc agcatcgcca gcaggcgatc gtgcaaaact tcaacttcac gctgcgggct
    57721 atggcaaacg tggaaggtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    57781 acgggaaaac tcttcgatgt tcacaccagc aacggcacgg ttttccagtt ccagaatgtc
    57841 agactgaatg ttatgcagca ggttatctgg cgtgacatcg acaaaggcgt ccagctcctg
    57901 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttac cccatgaagc
    57961 cagcagcggg ttgccgacat cctgttcacc atcgctgtta aagagctgcc cggcattttc
    58021 gctatcacga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgggtcag
    58081 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    58141 aaacaggaga tggatttcaa tatgtttacc cagcgcctgt agcgcctgga gataaacagg
    58201 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    58261 cgcggactcc agcgtttcga taaagcgctg atagagattg gcgcggtgcc agcgcggttg
    58321 cccgagttga tgggtatatt ccaccaacgc cttccacaac ggcgcttgcc aggcctgtgc
    58381 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    58441 gaccagatac tggtcaaaca ggtccgccgc ttttgaagaa agctggaaca gttttcgctt
    58501 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgat ccagcaattg
    58561 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    58621 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgcaggca gcggaaaatc
    58681 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    58741 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    58801 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    58861 agcggctcct gactactgac gattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    58921 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    58981 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccagccatg
    59041 ccgccagagc tgttggtact ggtttctgct ggcaagactg ttcattaatg ttcgctggaa
    59101 acccgataac gcagtgacaa tcatcaccat caacaccatc gccaacatta cctccggcag
    59161 gctaaagcct tgttgattct tcagggaagc tgacataacg ccctctcttt cagcggacaa
    59221 aaatcgctcc agccgcgtgg cgaaaaaaca atgtttccat caatgacttc gcctgttcgc
    59281 cacaacaaaa cgccttcata atcggcaatc aataaggctt cattatctgc cagtaaacgc
    59341 aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc
    59401 caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac
    59461 tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gttggctcat tccttgtaat
    59521 agcaagctac ctaaaaccag cagcatcagg accagagcca gtgacgaaac acccttttcg
    59581 cggttcacag gttgaatcct gtcacgctat agctggcatt caccacggtt tgcggttcag
    59641 acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgat
    59701 gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct
    59761 taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc
    59821 caatctggtc agactctttt accggttcgc gatcccagat accgttactg ttcgcatccc
    59881 actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc
    59941 catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt
    60001 catcttccag cgccagctta cgggtgctcg ttaaactttc acgctgtaac gcaggcagaa
    60061 agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca
    60121 gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgac aaagccggag
    60181 tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg
    60241 cccggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat
    60301 ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc
    60361 cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg
    60421 gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctggct
    60481 ggcggtttgc catagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc
    60541 gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg
    60601 tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc
    60661 ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc
    60721 gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg gctctggcag
    60781 gatgtttcgt aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc
    60841 aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa
    60901 cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct
    60961 gtacaggtgc gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg
    61021 cgccatcata tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac
    61081 gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa
    61141 gaacgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac
    61201 aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc
    61261 aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggtccgag
    61321 gtcgccgttt tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc
    61381 aatgttagtg tcgccctgca gaaaccacag cagttcgtgg atgatggaac gcaggtggca
    61441 acgtttagtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa
    61501 aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt
    61561 ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg
    61621 acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg
    61681 ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa
    61741 ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagccagaga cagctcccat
    61801 tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag
    61861 cagctcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg
    61921 ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat
    61981 ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag
    62041 acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaaa cctggaagaa
    62101 ggaacgttta gtacggcggg caaagataat catcaccacg ataacgccaa tcaggccgcc
    62161 gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg
    62221 cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc
    62281 cgcatagagt aagttttcaa cttcattttt ggtccagccg ctgcccggac gattcgcccg
    62341 tcgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg
    62401 aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct
    62461 actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat
    62521 gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg
    62581 gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag
    62641 cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag
    62701 ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag
    62761 caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc
    62821 aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat
    62881 tccatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag
    62941 actgtcataa atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt
    63001 cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa
    63061 taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat
    63121 catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat caacttcatc
    63181 gaggcttgtg accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag
    63241 catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg
    63301 ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc
    63361 gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata
    63421 ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt
    63481 gcgataaaga ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa
    63541 accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac
    63601 gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg
    63661 cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt
    63721 cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc
    63781 acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa
    63841 gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa
    63901 acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg
    63961 acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa
    64021 ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc
    64081 aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc
    64141 gaaaatagcc gccgtttctt tttgtgcacc ggcggcaaag cgtttgctgt agcggcgaaa
    64201 ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag
    64261 cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc
    64321 aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa
    64381 cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag
    64441 gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc
    64501 aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt
    64561 tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc
    64621 cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc
    64681 gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc
    64741 caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt
    64801 taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt
    64861 gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt
    64921 tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta
    64981 gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca
    65041 gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag
    65101 ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac
    65161 caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc
    65221 tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc
    65281 tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca
    65341 cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc
    65401 atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca
    65461 ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac
    65521 agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc
    65581 tgtggataga gttgtgaaga actacggaat tactacggga aaacc
//
LOCUS       DAMZII010000008        65243 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZII010000008 DAMZII010000000
VERSION     DAMZII010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.008"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            44..676
                     /gene="comR"
                     /locus_tag="POA77_000472"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="POA77_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDI6363957.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="POA77_000473"
     CDS             complement(738..1277)
                     /locus_tag="POA77_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI6363958.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="POA77_000474"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="POA77_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDI6363959.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3208..3750)
                     /locus_tag="POA77_000475"
     CDS             complement(3208..3750)
                     /locus_tag="POA77_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6363960.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="POA77_000476"
     CDS             complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="POA77_000476"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDI6363961.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="POA77_000477"
     CDS             complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="POA77_000477"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDI6363962.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="POA77_000478"
     CDS             complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="POA77_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDI6363963.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6269..6646)
                     /locus_tag="POA77_000479"
     CDS             complement(6269..6646)
                     /locus_tag="POA77_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDI6363964.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="POA77_000480"
     CDS             complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="POA77_000480"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDI6363965.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7342..9531
                     /gene="fhuE"
                     /locus_tag="POA77_000481"
     CDS             7342..9531
                     /gene="fhuE"
                     /locus_tag="POA77_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDI6363966.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="POA77_000482"
     CDS             complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="POA77_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDI6363967.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11319..12116)
                     /locus_tag="POA77_000483"
     CDS             complement(11319..12116)
                     /locus_tag="POA77_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6363968.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12127..13131)
                     /gene="holB"
                     /locus_tag="POA77_000484"
     CDS             complement(12127..13131)
                     /gene="holB"
                     /locus_tag="POA77_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI6363969.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13128..13769)
                     /locus_tag="POA77_000485"
     CDS             complement(13128..13769)
                     /locus_tag="POA77_000485"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI6363970.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="POA77_000486"
     CDS             complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="POA77_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDI6363971.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="POA77_000487"
     CDS             complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="POA77_000487"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDI6363972.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="POA77_000488"
     CDS             complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="POA77_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI6363973.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="POA77_000489"
     CDS             complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="POA77_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6363974.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="POA77_000490"
     CDS             complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="POA77_000490"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDI6363975.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="POA77_000491"
     CDS             complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="POA77_000491"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6363976.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="POA77_000492"
     CDS             complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="POA77_000492"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDI6363977.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="POA77_000493"
     CDS             complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="POA77_000493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI6363978.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="POA77_000494"
     CDS             complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="POA77_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI6363979.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="POA77_000495"
     CDS             complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="POA77_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDI6363980.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22298..22882
                     /gene="yceF"
                     /locus_tag="POA77_000496"
     CDS             22298..22882
                     /gene="yceF"
                     /locus_tag="POA77_000496"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDI6363981.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="POA77_000497"
     CDS             complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="POA77_000497"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDI6363982.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24526..27711
                     /gene="rne"
                     /locus_tag="POA77_000498"
     CDS             24526..27711
                     /gene="rne"
                     /locus_tag="POA77_000498"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDI6363983.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="POA77_000499"
     CDS             complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="POA77_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDI6363984.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="POA77_000500"
     CDS             complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="POA77_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDI6363985.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="POA77_000501"
     CDS             complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="POA77_000501"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDI6363986.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="POA77_000502"
     CDS             complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="POA77_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDI6363987.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="POA77_000503"
     CDS             complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="POA77_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDI6363988.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="POA77_000504"
     CDS             complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="POA77_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDI6363989.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="POA77_000505"
     CDS             complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="POA77_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDI6363990.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="POA77_000506"
     CDS             complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="POA77_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDI6363991.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="POA77_000507"
     CDS             complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="POA77_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDI6363992.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="POA77_000508"
     CDS             complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="POA77_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDI6363993.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="POA77_000509"
     CDS             complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="POA77_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDI6363994.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37993..38652
                     /gene="flgA"
                     /locus_tag="POA77_000510"
     CDS             37993..38652
                     /gene="flgA"
                     /locus_tag="POA77_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDI6363995.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38728..39021
                     /gene="flgM"
                     /locus_tag="POA77_000511"
     CDS             38728..39021
                     /gene="flgM"
                     /locus_tag="POA77_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDI6363996.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39026..39442
                     /gene="flgN"
                     /locus_tag="POA77_000512"
     CDS             39026..39442
                     /gene="flgN"
                     /locus_tag="POA77_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDI6363997.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMVQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="POA77_000513"
     CDS             complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="POA77_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDI6363998.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41127..42050)
                     /locus_tag="POA77_000514"
     CDS             complement(41127..42050)
                     /locus_tag="POA77_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6363999.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42052..42699)
                     /locus_tag="POA77_000515"
     CDS             complement(42052..42699)
                     /locus_tag="POA77_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDI6364000.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="POA77_000516"
     CDS             complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="POA77_000516"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6364001.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43530..44738
                     /gene="mdtH"
                     /locus_tag="POA77_000517"
     CDS             43530..44738
                     /gene="mdtH"
                     /locus_tag="POA77_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDI6364002.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44802..45449
                     /gene="grxB"
                     /locus_tag="POA77_000518"
     CDS             44802..45449
                     /gene="grxB"
                     /locus_tag="POA77_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDI6364003.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45583..46143
                     /locus_tag="POA77_000519"
     CDS             45583..46143
                     /locus_tag="POA77_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6364004.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46249..47295
                     /gene="pyrC"
                     /locus_tag="POA77_000520"
     CDS             46249..47295
                     /gene="pyrC"
                     /locus_tag="POA77_000520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI6364005.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47369..47614
                     /gene="dinI"
                     /locus_tag="POA77_000521"
     CDS             47369..47614
                     /gene="dinI"
                     /locus_tag="POA77_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDI6364006.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47904..48158
                     /gene="bssS"
                     /locus_tag="POA77_000522"
     CDS             47904..48158
                     /gene="bssS"
                     /locus_tag="POA77_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDI6364007.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48273..49391
                     /gene="solA"
                     /locus_tag="POA77_000523"
     CDS             48273..49391
                     /gene="solA"
                     /locus_tag="POA77_000523"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDI6364008.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49439..49552
                     /locus_tag="POA77_000524"
     CDS             49439..49552
                     /locus_tag="POA77_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDI6364009.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49813..50379
                     /locus_tag="POA77_000525"
     CDS             49813..50379
                     /locus_tag="POA77_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI6364010.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPAPLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50383..50958
                     /locus_tag="POA77_000526"
     CDS             50383..50958
                     /locus_tag="POA77_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDI6364011.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51000..52052)
                     /locus_tag="POA77_000527"
     CDS             complement(51000..52052)
                     /locus_tag="POA77_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI6364012.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52277..53197
                     /gene="lpxL"
                     /locus_tag="POA77_000528"
     CDS             52277..53197
                     /gene="lpxL"
                     /locus_tag="POA77_000528"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDI6364013.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53369..54595
                     /gene="mdtG"
                     /locus_tag="POA77_000529"
     CDS             53369..54595
                     /gene="mdtG"
                     /locus_tag="POA77_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDI6364014.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            54678..55052
                     /locus_tag="POA77_000530"
     CDS             54678..55052
                     /locus_tag="POA77_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDI6364015.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55053..55280)
                     /locus_tag="POA77_000531"
     CDS             complement(55053..55280)
                     /locus_tag="POA77_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI6364016.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55453..57996)
                     /gene="mdoH"
                     /locus_tag="POA77_000532"
     CDS             complement(55453..57996)
                     /gene="mdoH"
                     /locus_tag="POA77_000532"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDI6364017.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(57989..59524)
                     /gene="mdoG"
                     /locus_tag="POA77_000533"
     CDS             complement(57989..59524)
                     /gene="mdoG"
                     /locus_tag="POA77_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDI6364018.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59918..61075
                     /gene="mdoC"
                     /locus_tag="POA77_000534"
     CDS             59918..61075
                     /gene="mdoC"
                     /locus_tag="POA77_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDI6364019.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61083..62564)
                     /gene="clsC"
                     /locus_tag="POA77_000535"
     CDS             complement(61083..62564)
                     /gene="clsC"
                     /locus_tag="POA77_000535"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDI6364020.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62506..63039)
                     /gene="ymdB"
                     /locus_tag="POA77_000536"
     CDS             complement(62506..63039)
                     /gene="ymdB"
                     /locus_tag="POA77_000536"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDI6364021.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEEVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63134..63445)
                     /locus_tag="POA77_000537"
     CDS             complement(63134..63445)
                     /locus_tag="POA77_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6364022.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63566..63898)
                     /gene="csgC"
                     /locus_tag="POA77_000538"
     CDS             complement(63566..63898)
                     /gene="csgC"
                     /locus_tag="POA77_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDI6364023.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63957..64412)
                     /gene="csgA"
                     /locus_tag="POA77_000539"
     CDS             complement(63957..64412)
                     /gene="csgA"
                     /locus_tag="POA77_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDI6364024.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(64453..64908)
                     /gene="csgB"
                     /locus_tag="POA77_000540"
     CDS             complement(64453..64908)
                     /gene="csgB"
                     /locus_tag="POA77_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDI6364025.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15928 a        17371 c        15997 g        15947 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 tgattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttta tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
     3001 aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggcgata tagggtgaag
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagaggcgca tcaggcattg atgctcgaat
     3121 gtcggatgcg gcgtgaacgc cttatccgac ccacagaatc cggagcgcat caggtaccgg
     3181 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
     3241 taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
     3301 ataatgatgc agctcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
     3361 aatcaccagg caacgatcgc gattcttctc acggaagttg gtcacacact tagtggcaat
     3421 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
     3481 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
     3541 gcccagacca acgccgcaaa tcagcggacg ctcgtcgacg ttcagttgca acattttgtc
     3601 cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
     3661 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
     3721 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
     3781 ctgctttctc ttcctgccag cgttcatgta actgattcag acgggtgctg atcgctttcc
     3841 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
     3901 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
     3961 tgcagaccag gatcatatcg caacccgcat ccagtgatgc ctgcccgcgt tcggcataac
     4021 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
     4081 ccagttcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc ggacgcggat
     4141 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
     4201 aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg
     4261 gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg
     4321 ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt
     4381 gtggatcggc atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acatccagca
     4441 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
     4501 cctcttgcgc cagtttgccg ccctcttcca ttcctgacag cgcagcgaat gattgcgccg
     4561 ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg
     4621 ccaccaccag atgattgcgc gaagctgcgc ggatctggcg caccagttca cgtaactggg
     4681 caggatcatg atagttacgc gtaaagagaa tcagccctcc caccagcgga tgcgcaagta
     4741 tttcacgctc ttccgcgtcc agttcgtaac ctttgacatc caacattact ggacccacac
     4801 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
     4861 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
     4921 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
     4981 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac cgccgccagt
     5041 tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg
     5101 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
     5161 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
     5221 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
     5281 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
     5341 gtttttaccg cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
     5401 ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
     5461 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
     5521 agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
     5581 aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc tgcgaaacgg cacctttacc
     5641 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
     5701 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
     5761 tatggcttta ctacgggtac ctaaactgtc ctgcggcgac aaacctaact gttgcttagc
     5821 catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg
     5881 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
     5941 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
     6001 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg ccgtttgatc
     6061 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
     6121 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
     6181 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
     6241 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
     6301 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atgctaacgc
     6361 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     6421 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
     6481 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     6541 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     6601 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     6661 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     6721 ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc
     6781 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     6841 agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
     6901 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     6961 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     7021 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     7081 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
     7141 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
     7201 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttacaaacaa
     7261 aattattcgc acactaaata taaccgctca tcaccctcct ccggcatgag cctgacgaca
     7321 acataaacca agagatttca aatgctttca acacaattta acagggataa tcaatatcaa
     7381 gccagcacca aaccatcact acttgccggt tgcatagcat tggcactatt accttctgcc
     7441 gcttttgccg ctccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca
     7501 gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg
     7561 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
     7621 cagttacaaa cgctgggcga agtgatggaa aacacgctgg gaatcagcaa aagtcaggcg
     7681 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
     7741 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
     7801 ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat
     7861 ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat
     7921 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
     7981 ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca
     8041 tggctggacc gctacaacag tgaaaagacc ttcttctcgg gcattgtcga tgctgattta
     8101 ggcgatctta cgacgctttc agccggttac gaatatcagc gcattgatgt taatagccct
     8161 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgtgca
     8221 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
     8281 ctgaagcagc ggtttgctga tacctggcaa gcgacactga atgccaccca ctctgaagtc
     8341 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
     8401 gttgggccat acagtaatta tggacctggc tttgattatg tcggcggcac cggttggaac
     8461 agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtattta tgaattgttt
     8521 ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa tcgttacttc
     8581 agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat
     8641 ttcccacaaa ccgactggtc accacagagc ctggcgcagg acgataccac acatatgaaa
     8701 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
     8761 cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
     8821 ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc
     8881 tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc
     8941 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
     9001 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
     9061 ggcagcaacg gcgaaaccgc ctataaagca gtggatggga cagtcagtaa aggggtggaa
     9121 tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
     9181 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
     9241 atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac
     9301 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
     9361 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
     9421 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga aggttctatc
     9481 gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg aataacacct
     9541 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacaga ttagtggtta
     9601 cggatgtact catccatctc ggttttcagg ttgtcggatt tagtaccgaa aatcgcctga
     9661 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc
     9721 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
     9781 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtc
     9841 gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
     9901 agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat cgggaacagc
     9961 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
    10021 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
    10081 tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata
    10141 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt
    10201 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
    10261 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
    10321 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
    10381 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
    10441 aacgcaacaa ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca
    10501 atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg
    10561 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
    10621 aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag tacgccagta
    10681 tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
    10741 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
    10801 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
    10861 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
    10921 gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc
    10981 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
    11041 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta
    11101 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt
    11161 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
    11221 tatgtcacaa tatttcaaac gtggttattc accgcccaat tttcggcggt gaactttact
    11281 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
    11341 gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
    11401 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
    11461 ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat
    11521 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
    11581 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
    11641 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
    11701 agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca
    11761 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa
    11821 taatccagcc cggtttctcc cagcgctaca acaccctctt ctgccgccag acggcgtaaa
    11881 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
    11941 ttgtcacgtt cgcctaccag atcccgcatg tgtaaatagc ccggtaatgt tgtggcgact
    12001 gccagacaaa aattcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
    12061 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg
    12121 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
    12181 cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtgacagaca ttaactgttc
    12241 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
    12301 cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcgc catgatggcg
    12361 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg
    12421 attaagggcc gccagcagcg aataccagtc gcccgatggt acgctatatg ccaacgcctg
    12481 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg
    12541 cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg
    12601 tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg
    12661 actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc
    12721 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg ttagtaaggc
    12781 agcatcggtt acccagacaa ctttcgcacc acctaagcgt gcgtgctcat tcagcttttc
    12841 ggtgacctca cgtaccgcat caatgcccaa cgcatttttc cctttttcgg gagccagggt
    12901 gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    12961 tttgtgaccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    13021 atcgcccatg cccggtaacg cctgaatgag cagcgcatgg tgacctcttc cggcctgata
    13081 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    13141 tccttcaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    13201 gtggcatcaa tggtatgaat gcttttatct tgtgctgcca gttccagata gcgggcgcgg
    13261 gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc
    13321 gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa
    13381 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg
    13441 ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    13501 agcgcaggtt tgatgactgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact
    13561 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    13621 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    13681 agttgctcga gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc
    13741 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    13801 cacagacttg ttatgactgg caagattggt attaaacgtg tgaccacctt taccatcggc
    13861 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    13921 cggcgtagct attgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg
    13981 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    14041 ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac
    14101 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag
    14161 ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    14221 tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat tggcggtata
    14281 catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    14341 ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg
    14401 caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc
    14461 ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    14521 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac
    14581 ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt
    14641 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    14701 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    14761 aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    14821 tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt
    14881 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga ggcttgcact
    14941 tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg
    15001 ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg ccagaacaaa
    15061 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    15121 tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag atgtttaata
    15181 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg
    15241 ttacgcaaac ggtcgtaatg tgcaggataa accgtaacgg agagaatccg cgttgccggt
    15301 ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc
    15361 acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag
    15421 tcacagaaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac
    15481 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    15541 gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct
    15601 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    15661 ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca acttagatct
    15721 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt
    15781 ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg
    15841 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga
    15901 tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta
    15961 ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    16021 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    16081 ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg
    16141 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    16201 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    16261 gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    16321 gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    16381 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatgac
    16441 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    16501 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    16561 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    16621 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    16681 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    16741 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    16801 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    16861 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    16921 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat
    16981 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    17041 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    17101 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    17161 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    17221 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    17281 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    17341 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    17401 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    17461 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    17521 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    17581 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    17641 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    17701 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    17761 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    17821 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    17881 aaacagatga aaggttggtt tcgataatat cgttccactc ttcatctttc attcgcatta
    17941 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    18001 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    18061 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    18121 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    18181 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    18241 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    18301 caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc gacttcatag agatgttcta
    18361 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    18421 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    18481 caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    18541 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agagcgcgtt
    18601 ttgcgcccgc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    18661 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    18721 cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcgcccg
    18781 tgccttctgg tacggcttct tgcatgaact tgccgcgcat ctcaaccaga cgcaccgcat
    18841 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    18901 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagagg
    18961 cagtcaacag cgcaggctga gtttgccagg ttttattcag ttcttcagct ggcccctgct
    19021 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    19081 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    19141 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg
    19201 agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    19261 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    19321 ggcgatccag cgtcaccacg acattgtcca tagacatacc gagttttttc gccgttgcac
    19381 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    19441 tatttgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    19501 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacacgg tcagcattag
    19561 gcagcgtcag caactcacca tagctaccgt cggcatgcag atgggtggag atgattcccg
    19621 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    19681 cacgatcggt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    19741 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc
    19801 aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc cagcatgctt tgaatctgac
    19861 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    19921 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    19981 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    20041 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    20101 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    20161 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    20221 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    20281 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    20341 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    20401 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    20461 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    20521 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    20581 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    20641 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc
    20701 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    20761 tcttcagcca ggactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    20821 acgttggccc ctaaatcgag aaccaccgtt ttgccctttt gctgatgcgg caataccgtc
    20881 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    20941 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccaactcc
    21001 agggccacgc gcattgaact cccacgactg gcgcggatag cttgcgaagg ccggacatca
    21061 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    21121 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    21181 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    21241 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    21301 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    21361 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    21421 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    21481 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    21541 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    21601 atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag accatgtccg
    21661 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    21721 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    21781 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    21841 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    21901 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    21961 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    22021 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    22081 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    22141 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    22201 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    22261 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    22321 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    22381 accagaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    22441 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    22501 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    22561 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    22621 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    22681 tcacctgagc gaggcggaga ttgataatta cgtgcgtaaa gagcatcccc tgcactgcgc
    22741 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag aggggcgtga
    22801 tcctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    22861 gaaaaacccg ctgatgggat agttttttgc gccggatgcg gttacttcgc atccggcaat
    22921 cggtgctggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    22981 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    23041 ccatcggcgc ttcgatgcgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    23101 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    23161 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    23221 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    23281 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    23341 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    23401 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    23461 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    23521 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    23581 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    23641 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    23701 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    23761 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    23821 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    23881 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    23941 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    24001 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    24061 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    24121 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    24181 ttgttaaaac aagaaatttt acggaatacc ccattttgcc cgaccgatca tccacgcagc
    24241 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    24301 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    24361 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    24421 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    24481 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    24541 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    24601 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    24661 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    24721 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    24781 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    24841 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    24901 tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg tatcgaaggc
    24961 gacgaccgta ccgaattaaa agaagcactg gcgagccttg aactgccgga aggcatgggg
    25021 cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc
    25081 ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    25141 ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac
    25201 atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct
    25261 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    25321 ttcagccact accagatcga gtcgcagatc gaatccgcct tccagcgtga agtccgtctg
    25381 ccgtccggtg gttccattgt tatcgacagc accgaagcgt taaccgccat cgacatcaac
    25441 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    25501 gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    25561 gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    25621 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    25681 gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat ccagtcatca cgtttgtccg
    25741 cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    25801 ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    25861 ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt
    25921 caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc gcactaccac
    25981 gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg
    26041 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa
    26101 caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac gccagctgaa
    26161 cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc agcaactcct
    26221 gcacaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt
    26281 gaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa
    26341 caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc
    26401 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    26461 gcacagcagc agactgccga gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa
    26521 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    26581 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    26641 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    26701 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    26761 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    26821 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    26881 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    26941 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    27001 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    27061 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    27121 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    27181 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    27241 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    27301 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    27361 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    27421 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    27481 gcagcagaag ttgaaacggt agccgcggtt gaaccagaga tcaccgttga gcataaccac
    27541 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    27601 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    27661 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    27721 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg aatcaacata
    27781 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    27841 cgccccggcg atcccagagc aggcagacaa aacataccca gtttcatgat atgttttaaa
    27901 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    27961 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    28021 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc
    28081 cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaactgtaa ggacattatt
    28141 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    28201 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    28261 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    28321 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgcct
    28381 aatactttct gctccaccga cgtatttccc tttctcttcg ctaaacggcg cagtctctgt
    28441 tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa
    28501 ctggtcacga agcccctgga tatccgtagc cagcgaggcc cggtcatcgt cactcaaagt
    28561 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    28621 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgcgcca gcgtgtactg
    28681 gctgttttgc gcctgtgcct gggagagaac aaccgcttgt gatgctgcaa tgggatcgtc
    28741 agaagggtta acgactcgct tacctgtcga catctgctcg ccgtacttca tccattccgc
    28801 ctgagaattg gtgatgccac gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    28861 cttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    28921 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    28981 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    29041 gtggcgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    29101 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    29161 agggcctgac cgttgcggtt atcgctatca cccgcatctt cttcgctcgc catcgctatt
    29221 ttcgcttcgt cggtgattaa tacatccatg ttgacgatag cgtcacttac tggtttcagc
    29281 gtgaagctgt cgttaacagc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    29341 actttaccgt tggcatccgg cgtcaccgta aaagtggtat tactggcaag gcgggtgacc
    29401 tgccactgat tattatcgaa cgagattttg taatcagtcg ccagtaccgc ggaggcatca
    29461 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    29521 ttaccgatag caaagaaatc ttcaccggca tcgccgttag catcaaatcc ggctttgtgt
    29581 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    29641 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    29701 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    29761 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    29821 ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa cgctgacttc tacgccaaca
    29881 atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc
    29941 cctgccccca cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat ttgtttagcg
    30001 taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg
    30061 cgcagatatt gatcggtggt tttaaactga ttcaccaacc cttctgattt cccaatcagc
    30121 gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa
    30181 tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac
    30241 atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgcagcacg taactggttg
    30301 gtaataaacg catcatactc acgctgcaca ccagaaacgt agacgccatt gccaacccag
    30361 ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg
    30421 gcaacgttat agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc
    30481 agtccgctca tggcgttatt aatcaagctg gacatggagg ttccttataa gccttcaatt
    30541 acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga ttatcaatgt
    30601 tcatactgta ggttttgctc accttgtcgc ttatcgattt catctgctga atcatgttgg
    30661 tgagtttgcg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc
    30721 cctgttccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgc gttaacagcc
    30781 caacgtaatc cgacaaggct tccagatacg agctgtagac gcgaaacttc gcttttactt
    30841 tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc cctttccagt
    30901 tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg cggcggattt
    30961 gccgttgccc ccaaccagat tccagtgccg cctgagcgag gatcaaatga tgtggcacac
    31021 cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat gctttactgt
    31081 cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg
    31141 cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct gctggcgtgg
    31201 actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct gcaagcccca
    31261 gacctttgcc cgccgtcatc tgttgggcaa tttgctggtc atacatactg gtatacagac
    31321 gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca
    31381 tcatctgcac gaacatccct tccacctggc gggccaccgg acggatattt gccgccggat
    31441 cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc gcacttgcca
    31501 gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact
    31561 ttgcattgat tgcaaaatag acatcagatc catcggcgta gcgcccagcg cattgagcgc
    31621 gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac cgccgctctg
    31681 gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa acggtgtatc
    31741 tggctggctg acattggcct gacgattgac tgttactgag agatttccct gcgctaccgc
    31801 gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg agttaatcac
    31861 tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatctg caaggaaacg
    31921 gacctgggaa ctgttgccac ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt
    31981 ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa
    32041 atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc tgggcaattc
    32101 acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact
    32161 gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata
    32221 cacctgactg tcaacgccct taagcggcgt catcaacaac gtacctccac gcaagctttt
    32281 ggcatttccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc caaacggagg
    32341 aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg
    32401 aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg
    32461 ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa tcagtgagtt
    32521 ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt
    32581 gaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc
    32641 gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca
    32701 ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg
    32761 ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc ctgattaatg
    32821 gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc
    32881 accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc gttgaacgtg
    32941 ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc
    33001 ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc agaggagctt
    33061 ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt
    33121 cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata
    33181 gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc cccctgcacc
    33241 agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg
    33301 gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca gtaaaccggt
    33361 gaacctgcca ccggttaagc cttagagttg cgtcagtttt tgcagcatct gatcggtggt
    33421 ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc
    33481 ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt
    33541 caggcccggc gtgctttcgt tcggtgcacc agaggattgc gtttcggtgt agaggttttc
    33601 gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg
    33661 aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat
    33721 acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac
    33781 cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgaaccatc
    33841 tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc tgttgttggt
    33901 ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc
    33961 gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat
    34021 ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac
    34081 gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt
    34141 ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac aagtagagca
    34201 gaagcacgcg acagtgtgaa tttcccctca ccctaaccct ctccccaaag gggcgaggga
    34261 atagtccgcg cgcttttatt gcaatgtcat caatcgttca attaactcat cgacagcagt
    34321 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
    34381 cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
    34441 agaacccccg acatcacacg caaggttgga tctgcctgca gtatcggccc acgcgtggcc
    34501 tggctttctg cgcttaaacg aaaaatgccg tcgtcaccgc gttgcacttc gctgcccgtg
    34561 gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattg
    34621 agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg cacagcaatt
    34681 ggcccagcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt gggatcaacc
    34741 tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggactgcac ggccagccag
    34801 ccatcctgct gcaatgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    34861 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg taggcagtga aagcccttcc
    34921 accggcactg cacgcaaggc gtttaactgt gcgcgaaaac cgggcgttga ggcattggcc
    34981 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    35041 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt
    35101 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    35161 gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg caccgttggt
    35221 cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    35281 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    35341 tacaatctgc cccagcagtt gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    35401 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    35461 aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    35521 gggatctgcg ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac
    35581 gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct
    35641 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    35701 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    35761 attgctggcg ctgaatggcg taacagtagg aagcggatcg ctggaattca ggttgatctg
    35821 catcgatgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt
    35881 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    35941 taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    36001 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    36061 gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    36121 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    36181 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    36241 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    36301 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    36361 cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    36421 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    36481 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cggcggtcag
    36541 ttcaccaata tcaatggtgc gcacaaccgc accattttta tcggtgatgg tggccgtaac
    36601 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagctccttc
    36661 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag
    36721 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagtgtggt
    36781 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    36841 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa
    36901 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    36961 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    37021 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacgttggcc
    37081 tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc
    37141 atttttacgt agccctttgc atctgccagc ggattacccg gttcataaac cagtttgtcc
    37201 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    37261 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    37321 ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    37381 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    37441 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    37501 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    37561 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg
    37621 atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    37681 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    37741 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    37801 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    37861 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    37921 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    37981 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    38041 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    38101 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca
    38161 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    38221 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    38281 tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    38341 ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    38401 gttgatgcca ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    38461 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    38521 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    38581 gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    38641 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    38701 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    38761 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgggcggca
    38821 aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    38881 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    38941 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    39001 caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc
    39061 cgctgtgctt aacgatctca aaacggtaat ggttcaagag cagcaacatc tctctatggg
    39121 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    39181 gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat
    39241 tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    39301 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    39361 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    39421 cggtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt
    39481 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    39541 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg
    39601 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    39661 gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt
    39721 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    39781 aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta
    39841 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    39901 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    39961 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    40021 ttaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa
    40081 gacgcaaccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    40141 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    40201 ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa
    40261 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca
    40321 tctgtttcac cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc aggaccagca
    40381 tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa
    40441 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    40501 caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg
    40561 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    40621 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    40681 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcga
    40741 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    40801 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    40861 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    40921 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    40981 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    41041 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    41101 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    41161 acgatacgtt gcgccagcac ggcctgttcg ccggcggttt gcggaactgt ctgattttgc
    41221 acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgttcaag agtgctctgc
    41281 caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata
    41341 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    41401 gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    41461 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    41521 cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    41581 agatcgtgtg gcccgacact attgctacga tgtttatcca ttcttagcga agctgcggta
    41641 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    41701 aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    41761 gccagcggtt tatcgacaca gacatgtaca cccgcattga gtaacgtact gaccacgtca
    41821 aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat
    41881 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    41941 ggcgaccagg ctccttgtaa catccagtca gacgctgccg ccagcaccgg taaccacgct
    42001 ttttgcgcaa tgccgcctaa tcccacaacg ccgatacgta attttttcac tttaatctcc
    42061 cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    42121 ttcgacgcgg gcctgtaaat caccatcaac cgcgttcgac atatccgtta ccgccggctg
    42181 atcctctacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    42241 ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc
    42301 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacctgg
    42361 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttccgctg cgctcagttt
    42421 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    42481 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    42541 ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    42601 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    42661 aatcacgcga gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    42721 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    42781 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    42841 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    42901 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    42961 caatcgaata accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg
    43021 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    43081 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc
    43141 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    43201 caagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    43261 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    43321 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    43381 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    43441 taaaaaaata ttgtccccta agtgatggga aaagtcttag cggcaatgca gaatagaaag
    43501 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg
    43561 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    43621 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    43681 ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    43741 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    43801 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    43861 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    43921 aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    43981 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    44041 ccgttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct
    44101 ccaccgtaca cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    44161 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    44221 tgccaattat ggtcaacgac gtggctggcg caccctctgc cgttaaatgg atgtatgcca
    44281 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    44341 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    44401 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    44461 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    44521 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    44581 tcggtggtgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    44641 tgatgctggg aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga
    44701 aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc ccctgactcc
    44761 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    44821 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    44881 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    44941 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gacatcgttc
    45001 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    45061 aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca
    45121 aatcggcatt cgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    45181 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    45241 tcagcgatga tttacgtgct ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    45301 aactttcgga agatgatatt cagctatttc cgctgctgcg taatctgacg ctggtagccg
    45361 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    45421 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggtagcc tgtattcgcg
    45481 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    45541 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    45601 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    45661 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    45721 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt
    45781 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    45841 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    45901 gaaaaaggcg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    45961 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    46021 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga agcaatggcg
    46081 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc atttactgat
    46141 taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    46201 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    46261 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    46321 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc
    46381 aatctggctc cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt
    46441 gatgccgtac ctgccgggca caatttcacc ccattgatga cctgttattt aacagattcg
    46501 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    46561 tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg
    46621 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    46681 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    46741 ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct
    46801 gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg
    46861 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc
    46921 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat
    46981 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc
    47041 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    47101 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    47161 ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa
    47221 agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg
    47281 tccgttaaac aataaaaatt gtagcttcct gttgtcatta ggttatttta cctgtataaa
    47341 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    47401 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    47461 tgcgtttcct gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc
    47521 ggttattggc gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    47581 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    47641 gtcgccccgg cttttttatt ttacttacaa attttgagta caaaatttct acacttcctc
    47701 aaaataattg cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata
    47761 aatcttaaaa gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact
    47821 tattggtata ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta
    47881 gtaacacgaa ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc
    47941 tcgtagggtg ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact
    48001 accagacccc aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa
    48061 ccactgatgt tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat
    48121 ccggtgattt cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa
    48181 aaggcacctc accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac
    48241 tctgcttaaa ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca
    48301 gcggttccgt aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa
    48361 tgaccgacgc ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa
    48421 ttcgccatgc ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc
    48481 tgtgggatga actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca
    48541 ttaacctcgg cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt
    48601 ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac
    48661 gcgtcccgga caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac
    48721 tggcgattaa aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact
    48781 gcccggtcac cgcaattcgt catgacgatg atggcgtaac tattgaaacc gttgacggtg
    48841 agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg
    48901 agctgcctgt ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata
    48961 gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg
    49021 gttttccggc agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc
    49081 attcagcgga tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc
    49141 cgttcttgcg caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct
    49201 atgataattc gcctgacgaa gattttatta tcgataccct acccggccac gataatacac
    49261 tgctcattac cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag
    49321 ctgccgattt tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc
    49381 gcttccaata atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat
    49441 gcgtcggctg ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt
    49501 tctctgggga ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc
    49561 actgacataa actgacaaat atttaatctc cctttcattt ggcgtgaaaa attgaatgag
    49621 attattcctg cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat
    49681 gaagattctt tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg
    49741 ttatttttgt gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta
    49801 ttggatgcac ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc
    49861 ttccattggc taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc
    49921 acgctcagtt attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt
    49981 attttgttaa tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg
    50041 ggtccgctgc cgagttattc gcccatgact cgtcttgccg caaaggctgg acatctcgca
    50101 ttgtacctat tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt
    50161 aaaccgatca gcgtttttgg ctggtttgac gtccccgcgc cccttgccga cgctggcgca
    50221 caggcagact ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtcc
    50281 gttatgcacg gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag
    50341 cgcatgctgg gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt
    50401 ggtttaacct tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa
    50461 attgacaaag aaggtcagca cgcctttgtt aatttccgca tccagcacct tggctatagc
    50521 tggttatacg gcacctttaa agatttcgac ggtactttta cctttgacga aaaaaatccg
    50581 gctgccgata aagtgaatgt gacaattaac accaccagcg tcgatactaa tcacgccgaa
    50641 cgcgataaac atcttcgcag tgcagatttc ctcaataccg caaaatatcc acaggcaaca
    50701 ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg
    50761 ctgaatggcg taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac
    50821 ccatggggtg gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc
    50881 aatatcaaga cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa
    50941 ggcgtacagc agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt
    51001 tattccgttg gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag
    51061 atcttattgc cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg
    51121 ctttcttcgc agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac
    51181 tgaataaaca gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg
    51241 ccgcactggt ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca
    51301 aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg
    51361 tattcaataa ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag
    51421 gcactggcct tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta
    51481 tgtgcctgca tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt
    51541 tcgagcgcgt tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat
    51601 agtgcatcgg gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca
    51661 ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc
    51721 atgcgcagta cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc
    51781 tctaaagccg ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt
    51841 acgctgattt gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc
    51901 gcggtaaaca gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac
    51961 ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttagcgcg
    52021 tcgttggaaa tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag
    52081 taaaaatccg cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta
    52141 catttgcgca ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag
    52201 ccacaaattt tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgaataggtt
    52261 gaaaaacagg attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg
    52321 ttattggtta acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc
    52381 ggttatctac cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg
    52441 cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg
    52501 ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggcat
    52561 ggcgtggttc tggccggacc gccgaatcgc ccgctggact gaagtgatcg gcatggaaca
    52621 cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac
    52681 actggaactg ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc
    52741 gaacgataat ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc
    52801 gatgctcgac cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt
    52861 ctggtacgca ccggatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc
    52921 cgttgagcag gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg
    52981 tctggtgccc ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct
    53041 gccgccagag tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa
    53101 agtggtagaa aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt
    53161 taaaacacgc ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag
    53221 aagggtatcc ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat
    53281 tgcgccccct ggaagtcggg cgcataatta gtgtgcttat cttttcttct tatgttcacc
    53341 gcgcctggcg caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa
    53401 ctggaaacga aacctgatcg tcgcctggct aggctgtttt cttaccggtg ccgccttcag
    53461 cctggtaatg cccttcttac ccctctacgt tgagcagctt ggcgttacgg gccactccgc
    53521 cttgaatatg tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc
    53581 accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc
    53641 tctcggcatg ggcatcgtga tggtgttgat gggactggca caaaatatct ggcagttttt
    53701 gatcctgcgg gcgcttcttg gtttacttgg cggatttgtc cccaacgcta atgctcttat
    53761 cgccacacaa gtaccgcgta ataaaagcgg ctgggcactg ggtacgctct ccacaggcgg
    53821 cgttagcggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt
    53881 acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt
    53941 ttgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt
    54001 ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacca cgttaatcat
    54061 ccaggtggcg acaggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg
    54121 taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc
    54181 tctgctaagc gcaccacgac tcggcaaact tggcgatcga atcggacccg aaaagatcct
    54241 gattacagcg ctgatctttt ctgtactgct gttgatccca atgtcttacg ttcagacgcc
    54301 attgcaactt gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc
    54361 cgtacagaca ctgttggttt acaactcgag caaccagatc gccgggcgta tcttcagcta
    54421 taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc
    54481 agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt tattcaacgc
    54541 agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt
    54601 cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac
    54661 tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac
    54721 gcgaagaatt tcttgcagac aaccccggca tcgacgccga agatgcgaat gtgcaacagt
    54781 tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg
    54841 ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg
    54901 tttttgatgg cgactatgat gagatagaga tacgccagga gtggcaggaa gagaatacat
    54961 tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac
    55021 gcgcagcagc tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg
    55081 atcggcagca gtaaagtatc aaaaaccaga gagaatggca gatcaaggat cgtgacataa
    55141 cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt gccatgcccc
    55201 tgccccggta tagtgcgact aataatgctg ccacagccac tcagcaaggt caccatgatg
    55261 ctcaccacaa ttaaacgcat ttgatacctc tttcgtaaaa aaataccggc ataacgccgg
    55321 tatttttatt ggctcgttgt ttttgccgga tgcggcgtga acgccttatc cgacccacaa
    55381 aatcttgcaa aatcaataaa ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag
    55441 gcaactacgt ttttattgcg aagccgcatc cggtttacgc aatgccagtg gattgagctt
    55501 tatcccttcg taataactca cccatgaaga atatctctcc ggggaattcc agacgcggaa
    55561 atgcagacgg gccatcgtca ccggatcgct tagcagcacc aggcgacgat cgcgattcag
    55621 cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga tttccagcac
    55681 cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa atgacgggtt
    55741 aaacactgcg tgcataaagc catcatcaag ggagcgttga cgattcatct caaggaaccg
    55801 atcggtatca accagcacct gcggcggcga atactcttcc gggatcagga acagtttcca
    55861 gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa acggtgacag
    55921 gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca gccacgccat
    55981 cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga acgcttcacc
    56041 ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc agccaaggaa
    56101 cgcgctgaca acgaagaccg tatggaacag catgcgtacc ggagccagca gcacggaaaa
    56161 aagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt ctttcgttcc
    56221 tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca ccatagtcga
    56281 agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga acaactgccg
    56341 tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg cagtagagag
    56401 cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg tcaggaacac
    56461 cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca tcaggttacc
    56521 gtggcaccag cggcggtcac gttttagctc atcaagcaag ttaggcggta attcttcata
    56581 agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca tcaacgccgc
    56641 ttctacaaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg gcagcggagc
    56701 cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc cccaatagtg
    56761 cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat acacgcgggt
    56821 cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg gcgacgactg
    56881 aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcata aacaatcacc
    56941 ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc cccagcgacg
    57001 gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc ggcgatagaa
    57061 aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt gctctgcgac
    57121 gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt tggcattccc
    57181 ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt tcacgtcttc
    57241 gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt catcgccaac
    57301 tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga agcccattaa
    57361 cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga tcaggatacc
    57421 ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct gaccaaccat
    57481 atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca tataccaggt
    57541 cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga tggtaccgac
    57601 ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca gatagcgcgg
    57661 cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg tacgccacgg
    57721 gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat cgcgcccttc
    57781 gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt ccagacgcgc
    57841 ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt cggcatccag
    57901 cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat
    57961 gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg tagctccagg
    58021 tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg gttttcttgg
    58081 catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg ttatagcgca
    58141 ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg accggggtat
    58201 cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg atagtaccgt
    58261 caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc gtttgttgca
    58321 cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt
    58381 taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa gcgacgatgt
    58441 tatcgttggt ttcatcgttg gttggaattt ctaccagctc aacgctgcct ttaccccact
    58501 cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg agatcttcaa
    58561 agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt tccatagaga
    58621 agctgctgac cgctaaatgt ttcgggttat tcaacggacg ccagatccat tcgccattac
    58681 cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta tttgcaggcg
    58741 acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc agtttgccga
    58801 ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc cctggcataa
    58861 ctacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac
    58921 gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga aattcttcac
    58981 ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa acctgaccag
    59041 caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg tttttatctt
    59101 tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt accgtgtctt
    59161 tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt cgtttgactg
    59221 cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc tgatggtaga
    59281 actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga ttaaactgga
    59341 tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg
    59401 cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca atactgaaag
    59461 cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa cgcattttca
    59521 tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg cttatatcga
    59581 tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt atcgttcagc
    59641 acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt gcttctccta
    59701 aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc aaccgtctta
    59761 cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac cttgaagcct
    59821 gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca taacccgaca
    59881 gggataagac gaaaaatccg agattagtta accatatatg aatccagtac ccgcgcaacg
    59941 tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc cttttcatat
    60001 ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccat cattgtggct
    60061 gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg ttatatccgg
    60121 ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt
    60181 cgaacgtgta ggtatcccga tgttaacagc catcccccta ctgacattac cgcaatttat
    60241 tatgctgcaa tatgtcaaag gaaaagcgga aagttggcct gggctgtcat tgtatgacaa
    60301 atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg tgttagtagt
    60361 catgacgacg ctgtgcgtat ggatatttaa gcgcatcaga aataatttag aaaattctga
    60421 taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat gcctcggcat
    60481 cggttatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc tgagtaatgg
    60541 catgttcaat tttattgtca tgcaaacgct gttttatttg ccgttcttta tcctcggcgc
    60601 actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc gtggctgtac
    60661 ccttgcagca gcattggcgt ttgtcgctta tttactcaac cagcgctatg gcagtggcga
    60721 tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt ggatggtgaa
    60781 tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg tgacttattt
    60841 tgttaacgca tcgctgttta tctatctggt tcaccacccg ttaacgctgt ttttcggcgc
    60901 atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc tgatatttgt
    60961 agtagggatt gcgataattc tgtatgaaat tcatttgcgc atcccgttac tgaagttttt
    61021 gttctctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc gttaagccac
    61081 atttacaata accattccac gggcaatatc gacgccagtc tgaccataac ccgcttccag
    61141 aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg atcaacccag
    61201 ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg gctctgaata
    61261 aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc catttcagta
    61321 ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt tttaccatcg
    61381 atgctaaagg ttttagcatg caggctggct ccggaattac cggttatgcc gcgatcgtgt
    61441 aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc atagcggagc
    61501 aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt agcggcaaga
    61561 gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag ttgcgccaca
    61621 cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact gggtgagccc
    61681 atgatatcga acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc
    61741 gccggatcat cacttaataa acgtgtcttc gcccagataa gcggcaatgt tccatcaacc
    61801 agatgattta taaatggact ggattccatt ttgcgtaaat aacgatgcgt catggcatcg
    61861 ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccctccgg gacatccagc
    61921 acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc atcggcaacg
    61981 tcctctacca cgggtcctat tgccatgaca tctaaatccg aaaaaagtgg ctcctcccct
    62041 gctccaaaat aggcatcacc aatatttcgt cctcccacca gggtcaccac gccatcgaca
    62101 gtgaaacttt tattgtgcat acggcgatta agacgggaaa agtcggtgat ataaccaagc
    62161 ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg tggatgactg
    62221 tcaagcaagc gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg
    62281 acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg acccgacata
    62341 tcgtcctgcc agatgtaata ctgaacatcg agcgtatgct ctgccatttc ggccagacga
    62401 taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca ctgaccggga
    62461 tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt catctccttg
    62521 ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga caaagtatac
    62581 ctcttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg cgatttcagc
    62641 cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag ccactgacgt
    62701 atagctgttt gccgccacca gtcgtaggct attgagatag gcatcctgca aaagctggtc
    62761 ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgcactacgg ctttagcggg
    62821 aagagcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct gctgttgcct
    62881 gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg ccccatcgac
    62941 gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa cggccagttt
    63001 ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt acaccttctt
    63061 gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc agcaacgtgc
    63121 gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc tgtttatcaa
    63181 gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc attaacgctt
    63241 tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt tctacgggtg
    63301 atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa tggatcaccc
    63361 ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aagcaaactg ccgagcataa
    63421 gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt catccttgag
    63481 ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct ctccagattc
    63541 atcttatgct cgatatttca acaaattaag acttttctga agagggcggc cattgttgtg
    63601 ataaatgaag tgactgtcca tcagaaacag taacaactat tttcacccga tcttccgggg
    63661 aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat gagagggtct
    63721 tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat atttgtactc
    63781 tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca tccccttgct
    63841 gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac gcattcatat
    63901 tcttctcccg aaaaaaaaca gggcttgcgc cctgtttctg taatacaaat gatgtattag
    63961 tactgatgag cggtcgcgtt gttaccaaag ccaacctgag tcacgttgac ggaggagtta
    64021 gatgcagtct ggtcaactgc agcaccgttg ccaccaccga actgtttaac agtcatttca
    64081 gaatttttgc cgttccactg atcaagagtg gcgctgttac cgaagccacg ttgggtcaga
    64141 tcgattgagc tgtcatctga gccctgacct acatctgcac cattaccacc gccatgctgg
    64201 gtaatagtca agtcagagtt acgggcatca gtttgcagag caagtgcaga gttaccgcca
    64261 ccgtactggt aaatgttcag ctctgaattt gggccgctat tattaccgcc accaccgtgg
    64321 ttaccgccgc cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt
    64381 gctgcaattg ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa
    64441 gtcgaatgga aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac
    64501 aattgccgtt ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg
    64561 gataatcatc gcagtattac cataagcacc ttgcgaaata ctggcatcgt tggcactgcc
    64621 cgcctgatca atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct
    64681 actaccttct tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact
    64741 attattcgtc ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc
    64801 atttaccgcg aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc
    64861 aggcgcaccc agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg
    64921 gacctggtcg tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctgta
    64981 ttttgttaac gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac
    65041 ccgcgcgttg tatttttctt tttttgttca taccaaagta ttaaaaccca cgtaaatacg
    65101 ctgttatcta cgcaaaaaat attttgtttt cttttaaatc tccgttttcc gctatcaaaa
    65161 atcaccagac agtcattttt cttgcccgtc gctgattgct gcgatatgtc ttgcgcacaa
    65221 gccgtgacaa acctcgtctt ttt
//
LOCUS       DAMZII010000009        63170 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZII010000009 DAMZII010000000
VERSION     DAMZII010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.009"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            102..1712
                     /gene="yeaV"
                     /locus_tag="POA77_000541"
     CDS             102..1712
                     /gene="yeaV"
                     /locus_tag="POA77_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDI6364026.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            1744..2868
                     /gene="yeaW"
                     /locus_tag="POA77_000542"
     CDS             1744..2868
                     /gene="yeaW"
                     /locus_tag="POA77_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDI6364027.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2924..3889
                     /gene="yeaX"
                     /locus_tag="POA77_000543"
     CDS             2924..3889
                     /gene="yeaX"
                     /locus_tag="POA77_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDI6364028.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3943..5058)
                     /gene="rnd"
                     /locus_tag="POA77_000544"
     CDS             complement(3943..5058)
                     /gene="rnd"
                     /locus_tag="POA77_000544"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDI6364029.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5140..6825)
                     /gene="fadD"
                     /locus_tag="POA77_000545"
     CDS             complement(5140..6825)
                     /gene="fadD"
                     /locus_tag="POA77_000545"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDI6364030.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(7030..7611)
                     /gene="yeaY"
                     /locus_tag="POA77_000546"
     CDS             complement(7030..7611)
                     /gene="yeaY"
                     /locus_tag="POA77_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDI6364031.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7651..8346)
                     /gene="tsaB"
                     /locus_tag="POA77_000547"
     CDS             complement(7651..8346)
                     /gene="tsaB"
                     /locus_tag="POA77_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI6364032.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8404..10314)
                     /locus_tag="POA77_000548"
     CDS             complement(8404..10314)
                     /locus_tag="POA77_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDI6364033.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSCQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10446..10790
                     /locus_tag="POA77_000549"
     CDS             10446..10790
                     /locus_tag="POA77_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6364034.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11153..11512
                     /locus_tag="POA77_000550"
     CDS             11153..11512
                     /locus_tag="POA77_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDI6364035.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTENMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11632..11811)
                     /locus_tag="POA77_000551"
     CDS             complement(11632..11811)
                     /locus_tag="POA77_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDI6364036.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11885..13246
                     /gene="pabB"
                     /locus_tag="POA77_000552"
     CDS             11885..13246
                     /gene="pabB"
                     /locus_tag="POA77_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDI6364037.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13250..13828
                     /locus_tag="POA77_000553"
     CDS             13250..13828
                     /locus_tag="POA77_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDI6364038.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            14012..15376
                     /gene="sdaA"
                     /locus_tag="POA77_000554"
     CDS             14012..15376
                     /gene="sdaA"
                     /locus_tag="POA77_000554"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI6364039.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGNEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15507..17105
                     /locus_tag="POA77_000555"
     CDS             15507..17105
                     /locus_tag="POA77_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6364040.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(17109..18665)
                     /gene="yoaE"
                     /locus_tag="POA77_000556"
     CDS             complement(17109..18665)
                     /gene="yoaE"
                     /locus_tag="POA77_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDI6364041.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            19128..20099
                     /gene="manX"
                     /locus_tag="POA77_000557"
     CDS             19128..20099
                     /gene="manX"
                     /locus_tag="POA77_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDI6364042.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20162..20962
                     /gene="manY"
                     /locus_tag="POA77_000558"
     CDS             20162..20962
                     /gene="manY"
                     /locus_tag="POA77_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDI6364043.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20975..21826
                     /gene="manZ"
                     /locus_tag="POA77_000559"
     CDS             20975..21826
                     /gene="manZ"
                     /locus_tag="POA77_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDI6364044.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21881..22339
                     /locus_tag="POA77_000560"
     CDS             21881..22339
                     /locus_tag="POA77_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDI6364045.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            22768..23334
                     /gene="mntP"
                     /locus_tag="POA77_000561"
     CDS             22768..23334
                     /gene="mntP"
                     /locus_tag="POA77_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDI6364046.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(23331..24140)
                     /gene="rlmA"
                     /locus_tag="POA77_000562"
     CDS             complement(23331..24140)
                     /gene="rlmA"
                     /locus_tag="POA77_000562"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDI6364047.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(24306..24515)
                     /gene="cspE"
                     /locus_tag="POA77_000563"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(24306..24515)
                     /gene="cspE"
                     /locus_tag="POA77_000563"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI6364048.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(24528..24671)
                     /locus_tag="POA77_000564"
     CDS             complement(24528..24671)
                     /locus_tag="POA77_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDI6364049.1"
                     /translation="MCGIFSKEVLSKHVDVEYCFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(25340..25627)
                     /locus_tag="POA77_000565"
     CDS             complement(25340..25627)
                     /locus_tag="POA77_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDI6364050.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(25702..25845)
                     /gene="mgrB"
                     /locus_tag="POA77_000566"
     CDS             complement(25702..25845)
                     /gene="mgrB"
                     /locus_tag="POA77_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDI6364051.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            26004..26243
                     /locus_tag="POA77_000567"
     CDS             26004..26243
                     /locus_tag="POA77_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364052.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(26386..27177)
                     /gene="kdgR"
                     /locus_tag="POA77_000568"
     CDS             complement(26386..27177)
                     /gene="kdgR"
                     /locus_tag="POA77_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDI6364053.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            27354..28727
                     /locus_tag="POA77_000569"
     CDS             27354..28727
                     /locus_tag="POA77_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364054.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(28773..29654)
                     /gene="htpX"
                     /locus_tag="POA77_000570"
     CDS             complement(28773..29654)
                     /gene="htpX"
                     /locus_tag="POA77_000570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDI6364055.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(29846..31894)
                     /gene="prc"
                     /locus_tag="POA77_000571"
     CDS             complement(29846..31894)
                     /gene="prc"
                     /locus_tag="POA77_000571"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDI6364056.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(31914..32612)
                     /gene="proQ"
                     /locus_tag="POA77_000572"
     CDS             complement(31914..32612)
                     /gene="proQ"
                     /locus_tag="POA77_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDI6364057.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(32709..33206)
                     /gene="msrC"
                     /locus_tag="POA77_000573"
     CDS             complement(32709..33206)
                     /gene="msrC"
                     /locus_tag="POA77_000573"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDI6364058.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            33336..34619
                     /gene="yebS"
                     /locus_tag="POA77_000574"
     CDS             33336..34619
                     /gene="yebS"
                     /locus_tag="POA77_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDI6364059.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            34588..37221
                     /gene="yebT"
                     /locus_tag="POA77_000575"
     CDS             34588..37221
                     /gene="yebT"
                     /locus_tag="POA77_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDI6364060.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            37301..38740
                     /gene="rsmF"
                     /locus_tag="POA77_000576"
     CDS             37301..38740
                     /gene="rsmF"
                     /locus_tag="POA77_000576"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDI6364061.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            38858..39094
                     /locus_tag="POA77_000577"
     CDS             38858..39094
                     /locus_tag="POA77_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDI6364062.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            39199..39390
                     /locus_tag="POA77_000578"
     CDS             39199..39390
                     /locus_tag="POA77_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDI6364063.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(39391..40047)
                     /gene="pphA"
                     /locus_tag="POA77_000579"
     CDS             complement(39391..40047)
                     /gene="pphA"
                     /locus_tag="POA77_000579"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI6364064.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(40443..40784)
                     /locus_tag="POA77_000580"
     CDS             complement(40443..40784)
                     /locus_tag="POA77_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDI6364065.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(40797..41669)
                     /gene="copD"
                     /locus_tag="POA77_000581"
     CDS             complement(40797..41669)
                     /gene="copD"
                     /locus_tag="POA77_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDI6364066.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(41673..42047)
                     /gene="yobA"
                     /locus_tag="POA77_000582"
     CDS             complement(41673..42047)
                     /gene="yobA"
                     /locus_tag="POA77_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDI6364067.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            42186..42416
                     /gene="holE"
                     /locus_tag="POA77_000583"
     CDS             42186..42416
                     /gene="holE"
                     /locus_tag="POA77_000583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDI6364068.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            42518..43174
                     /locus_tag="POA77_000584"
     CDS             42518..43174
                     /locus_tag="POA77_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDI6364069.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            43198..43860
                     /gene="exoX"
                     /locus_tag="POA77_000585"
     CDS             43198..43860
                     /gene="exoX"
                     /locus_tag="POA77_000585"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDI6364070.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(43857..45917)
                     /gene="ptrB"
                     /locus_tag="POA77_000586"
     CDS             complement(43857..45917)
                     /gene="ptrB"
                     /locus_tag="POA77_000586"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDI6364071.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(46126..46785)
                     /locus_tag="POA77_000587"
     CDS             complement(46126..46785)
                     /locus_tag="POA77_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDI6364072.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(47112..47468)
                     /locus_tag="POA77_000588"
     CDS             complement(47112..47468)
                     /locus_tag="POA77_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDI6364073.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(47535..47825)
                     /gene="yebG"
                     /locus_tag="POA77_000589"
     CDS             complement(47535..47825)
                     /gene="yebG"
                     /locus_tag="POA77_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDI6364074.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            47959..49137
                     /gene="purT"
                     /locus_tag="POA77_000590"
     CDS             47959..49137
                     /gene="purT"
                     /locus_tag="POA77_000590"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDI6364075.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(49193..49834)
                     /gene="kdgA"
                     /locus_tag="POA77_000591"
                     /gene_synonym="eda"
     CDS             complement(49193..49834)
                     /gene="kdgA"
                     /locus_tag="POA77_000591"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDI6364076.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(49871..51682)
                     /gene="edd"
                     /locus_tag="POA77_000592"
     CDS             complement(49871..51682)
                     /gene="edd"
                     /locus_tag="POA77_000592"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDI6364077.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(51917..53392)
                     /gene="zwf"
                     /locus_tag="POA77_000593"
     CDS             complement(51917..53392)
                     /gene="zwf"
                     /locus_tag="POA77_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI6364078.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            53730..54599
                     /gene="hexR"
                     /locus_tag="POA77_000594"
                     /gene_synonym="yebK"
     CDS             53730..54599
                     /gene="hexR"
                     /locus_tag="POA77_000594"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDI6364079.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            54727..56169
                     /gene="pyk"
                     /locus_tag="POA77_000595"
     CDS             54727..56169
                     /gene="pyk"
                     /locus_tag="POA77_000595"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI6364080.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(56301..57272)
                     /gene="lpxM"
                     /locus_tag="POA77_000596"
                     /gene_synonym="msbB"
     CDS             complement(56301..57272)
                     /gene="lpxM"
                     /locus_tag="POA77_000596"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDI6364081.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(57392..58714)
                     /gene="mepM"
                     /locus_tag="POA77_000597"
     CDS             complement(57392..58714)
                     /gene="mepM"
                     /locus_tag="POA77_000597"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDI6364082.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(58730..59662)
                     /gene="znuA"
                     /locus_tag="POA77_000598"
     CDS             complement(58730..59662)
                     /gene="znuA"
                     /locus_tag="POA77_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDI6364083.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            59741..60496
                     /gene="znuC"
                     /locus_tag="POA77_000599"
     CDS             59741..60496
                     /gene="znuC"
                     /locus_tag="POA77_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDI6364084.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            60493..61278
                     /gene="znuB"
                     /locus_tag="POA77_000600"
     CDS             60493..61278
                     /gene="znuB"
                     /locus_tag="POA77_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDI6364085.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSITISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(61425..62435)
                     /gene="ruvB"
                     /locus_tag="POA77_000601"
     CDS             complement(61425..62435)
                     /gene="ruvB"
                     /locus_tag="POA77_000601"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI6364086.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(62444..63055)
                     /gene="ruvA"
                     /locus_tag="POA77_000602"
     CDS             complement(62444..63055)
                     /gene="ruvA"
                     /locus_tag="POA77_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI6364087.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT        15263 a        16272 c        15608 g        16027 t
ORIGIN      
        1 caaataatca atttgttaaa cacaacatac aactcattac cctgcaaata attacaactg
       61 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
      121 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
      181 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
      241 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
      301 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
      361 tctcctggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
      421 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
      481 aggcactcga atttagcgtc ccttactcct tcttccactg gggcatcagc gcctgggcaa
      541 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcgtaaa aacaaaggtc
      601 tgagtctttc cggcattatt gccgcgatta ccggcgttcg cccgcaaggc ccatggggaa
      661 aactggttga tttgatgttc ctgatcgcca ctgtcggcgc actgactatt tcccttgttg
      721 tcaccgcagc aacctttact cgtggacttt ccgcgctgac cggtttaccc gataacttca
      781 ccgtgcaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcgtggattg
      841 gtatcaacaa cggtttgcaa cgtctgagca aaatggttgg ctggggcgcg ttcctgctgc
      901 cattactggt gctgattgtc ggcccaaccg aatttattac caacagcatc atcaatgcca
      961 tcggtctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
     1021 gttcatttac ccgcaactgg accgttttct actggctgtg gtggatctca tacacccctg
     1081 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
     1141 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
     1201 ccattcatca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
     1261 gcgaaacagc tgtacagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
     1321 catacctggg cgtgatgatt attttccttg cctcgcatat ggatgcggtg gcctacacca
     1381 tggctgcgac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
     1441 ttttctggtg cgtggtgatc actctgatcc cgctttccat cttgtttacc ggtgcttcgc
     1501 tggaaacgat gaaaaccacc gtcgtgctca cagcccttcc cttcctcgtc attttactgg
     1561 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
     1621 aagttgaaca ttatctcccg cagacaccgg ttgaagccct ggaaaaaacg ccagtgctcc
     1681 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
     1741 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
     1801 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcgtttga acacgaaaaa
     1861 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
     1921 aatgattatg tgacgcgtga gatcattggc gaaagcatcg tgctggtacg cggtcgtgat
     1981 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt
     2041 gaaggaaaag caaaaaatgt gattacctgc ccgtatcacg catgggcatt caaactcgat
     2101 ggcaatctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
     2161 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggacccc
     2221 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
     2281 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
     2341 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
     2401 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
     2461 tacggtttcg ccaaaccgtc cgaacagtcg tttaaatttg aagagggtac ggatgcggca
     2521 ttccacggtt tctggctgtg gccgtgcacg atgctgaacg tcaccccgat caaagggatg
     2581 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
     2641 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
     2701 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
     2761 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagtggcat ttccgaacat
     2821 ggtatcgccc atttccataa tctgctggcg caggtgttta aggactaatg acatcggcgg
     2881 cggtattttc cgccgctggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
     2941 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
     3001 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcattgtgca
     3061 gatgagcgat ggtgataacc agtacagcaa tgcgtattca ctactgagtt cgccgcatga
     3121 cacctcttgt tatcagattg ccgttcggct ggaggaaaac tcgcgcggcg gttcccgctt
     3181 tttgcatcag caggtaaaag tgggcgatcg gttaacgatt tcaacgccta ataacctgtt
     3241 tgcgctaatt ccctcagcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
     3301 tttcctgtcg cacatggcag agctgcaaca cagcgacgtc gactggcagc tacattactg
     3361 ctcgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cgcaggctga
     3421 gaaagttcac ttgcatcact catcaaccgg aacacgactg gaattagcgc gattattggc
     3481 ggatatcgaa cctggcacac acgtttatac ctgtggtccc gaggcgctaa atgaagcggt
     3541 aagaagtgaa gctgcgcgtc tggacatcgt cgccgatacg ctgcactttg agcaatttgc
     3601 tatcgaagac aaaaccggcg atgcctttac cctggtgctt gcccgttccg gaaaagagtt
     3661 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag ccgcgaaagt
     3721 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acagcaatac tggaaggtga
     3781 agctgaccat cgggatcagt attttagcga tgaagagcgt gccagccagc aaagtatgtt
     3841 gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg gatttgtagt ttgccaaagc
     3901 cggatgtggc gctgagcgcg cccagtccgg cttcggaaga ttttactgcg gatattcctg
     3961 caataaattg tgtaatgctt ccgccatcag ctcaccacgc cagccggaaa tcagctccgg
     4021 caaattgttc tgcggtttca gtttccagtg ccagttcagc agttggttga tttgccgacg
     4081 cgatgccagc aattcggcgc tgatcttatg cgtttcgctc acgtcagtaa tcagcgactt
     4141 aatcgcttta aacgctttac gataacccgg catgtccatc aggttaagca tcggctgcgg
     4201 taaggcctct tccggcaatg tctgcgcttt ttccaccagc gccagcagcg ttttaccgtg
     4261 aaagcggatt tcgctaccgg ataaacccag gctgtccagt tcgcctaaac ttcccggcat
     4321 ataacgcgct accgaccaca aatgctcttc acgcacgaca aagttcaccg ccagatcgcg
     4381 ctctcgcgcc ttgcgcagtc gccagtcggc taacagttgc agacaggcca gttggcgtgt
     4441 gcgtaactgc caggcattgg tgatatcacg ccaggcatct tccggcgcaa cgacttcctg
     4501 acgacgcatt tgcatcaggc ggcattcatc cagcgccgca ggtagccagc cggaggcctc
     4561 cgtttctacc ataagcttgg cggtgatcgg taacagatac cagacatccg ccgctgcgta
     4621 ttcacactga cgttcggtca gcggtctggc cagccagtcg gtgcgcgatt cactcttgtc
     4681 cagcgtaacg ccggaatact cttccaccat ggaagcgaaa ccccatgaca tcgggcgtcc
     4741 gcagaaggca gcaaggattt gcgtgtcaat caagggttgt ggtaattcgc caaagacatt
     4801 gaggaacact tccagatctt cactgcctgc atggagaaat tttgtgatgg acggatcgcg
     4861 caggatcgct ttcagcggtg accagtcggt gatcccgaga ggatcaatta gcgccagatg
     4921 ctcgccatcg aaaagttgaa tcaaccccag ctgcgggtaa taagtgcgcg tacgaacaaa
     4981 ttcagtatcc agggctatcg ccggaaaggc acggacggct tcacacaaag aagccagcgc
     5041 atcgtccgtg gtaatcattt ggtaattcaa attgttttct ctttagtggg cgtcaaaaaa
     5101 aacgccggat taaccggcgt ctgacgactg acttaacact caggctttat tgtccacttt
     5161 gccgcgcgct tcgtcacgta attctcgtcg caaaattttt ccgacgttag atttcggtaa
     5221 ctcatcacga aactccacca gcttcggtac tttgtagccc gtgagctgac ggcggcaaaa
     5281 ggtcaccagt gactcttcgg taagcgatgg atcttttttc actacgaaga ttttcaccgc
     5341 ttcaccactg gagccggaag gtacgccaac agccgcgact tcctgtacgc caggatgctg
     5401 catgacgaca tcttcaatct cgttgggata gacgttaaaa ccggaaacca gaatcatgtc
     5461 ttttttacga tcaacaatgc gcaggaatcc ttcttcatcc attaccgcga tgtcgccggt
     5521 gtgtaaccag ccatttttga tgatttcatc ggtagcatcg ggacgctgcc agtaacccag
     5581 catcacctgc ggtcctttga cacaaagctc acccggttga cctggtggta cttcattatc
     5641 atcatcatcc accagtttgg cttccgtcga cggcaccggc aaaccgatgc taccactatg
     5701 ataatcaata tcatatgggt taacgctgac cagcggcgca cactcggtaa ggccatagcc
     5761 ttccagcaga tactgtccgg tcagtttcac ccaacgctct gccaccactt gctgcactgg
     5821 catccctccg cctgcggaaa gatgcagact ggagaaatcc agctgctgga actctttatt
     5881 gttcagcaac gcattgaaca aggtgttaac gcccgtgata gcggtaaacg gatatttcgc
     5941 taactctttt accaaccctg gaatatcgcg cgggttagtg ataagcaggt tctgcccacc
     6001 cagttcgata aacagcaggc agttaatggt cagggcaaaa atgtgataca gcggcagtgc
     6061 cgttactacc agttctttac ctggatgcag caacggccca taggtggcgt tgacctgttc
     6121 caggttcgcc agcatattgc ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc
     6181 gccggtgtat tgcaggaagg ctaaatcttc cggcaccagt tcgggtttga cgtactgcat
     6241 ccggtagccg ttatgcagtg cgctacgaaa tgaaatggca tctggcagat ggtatttcgg
     6301 caccaaacgc ttgatgtatt taacaacgaa attgactacc gtgccttttg ccgtagatag
     6361 ctgatcgccc atacgggtca gaattacgtg ctgaacggcg gttttatcaa ccactttttc
     6421 cagtgtgtga gcaaagttag acacgataac aatcgccgat gcgccgctat cgttaagctg
     6481 atgctcaagc tcacgcgggg tatacaacgg gttaacgttt acgacgatca tcccggcacg
     6541 caaaatgcca aacagcgcca ccggatattg caataaatta ggcatcatca acgcaacgcg
     6601 atcgcctttc ttcagcccca acccttgttg caaataagcg gcaaacgcgc gactgcgttc
     6661 ttccagcttg cggaaggtca ttacctcccc catattcaca aacgcaggct gatcggcgta
     6721 gcgcgcgacc gactgctcaa acatatctac cagagattga taacggtcag ggttgatctc
     6781 cgtcggaacg tccgcgggat aacggttaag ccaaaccttc ttcaattctt cacctctaaa
     6841 atgcgtgttc gtcgtcatcg caaccccaaa tgatatacat gccgttaaca taatattaac
     6901 tcatcatacc agcttgataa ttacccaacg aaaaggttgc gaagcgcgtc actatttatt
     6961 tttatcttta ccgtaagaat gcagaaacag cggaccagcc gctgtttctt tttctttaaa
     7021 aacaagcaat tattcagtta caactgtttg tactctcgcg gggccgggat tataccagcc
     7081 ccatccgccg tagccatagg gccagccacg accgccataa aaccatggat caatcggctg
     7141 aggcggcata atcacctgct gggttaaatg ccaacgtttg taacccgtta cttgcatcac
     7201 cataaattta tagggcgtgt tgccgatttt tccgtcaacc gcaccagtga ttggcccgac
     7261 taccgtaacc agttgtccac ggaaatccac cgggtccaga aaaccgttca catcggcata
     7321 aatgcgaccg cgagaaggtt ctcccagcgt cggtctggca ccgctgtcca gcggtacggt
     7381 agcaatttcc aggcgggttt tcccttgctg gttttgtacc gcaaccactt tgccaccaaa
     7441 gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc acccgaacta aatcttgttg
     7501 cggcgtggga ctgctgcctt taatggcgtc cggcacagtg acacaaccgc tcagcattag
     7561 cgcaaacgta cctgccagta tgcctttgat aacattcttt tgaaccgcca tgatgcgact
     7621 cctttttttc tcagggcata ctcttaagat tcattctttg cccggaagtt tcttccatgc
     7681 gacgttgtta cgtaaataaa ccggttcggc atgttccacc gccaccgttt taccctcggc
     7741 aaacatctga caagcaatcg gcagcatatc ttctgcagcg gggagtaaca cttcgccatc
     7801 gcgcaaaacc agtccgctct ctttaccgag atccggccag gcttgccagc ctgtacctac
     7861 cgtcacccat tccccggaaa gctgttgcat tcgttcatga acgagttcgg gtttgagtac
     7921 ggcttcggtt tcttcaccgt gccagatacc gttttcatca cgctgatatt cggcccagta
     7981 aacttcgccc attcgcgcgt caatggctga cagcacgcgt gttgcgccgt ttttgcgcca
     8041 cgccccttgc gccatcgtca ttagcgtgga gacgccaatc atcggtaatt ccgcgccaag
     8101 cgccagccct tgcgcgatgc caataccaat gcgcacgcct gtaaagctac cggggccgcg
     8161 cccgtaagcc agagcgttaa tatcagtcag ggaagttccg ctggtggtca ggatatcctg
     8221 caccatcggt aagattcgtt gagtatgttc acgagggcaa agctcaaaat gagcgttgac
     8281 agtaccgtcg ttccacaggg cgacagagca ggcctctgtc gcggtatcga tagccagaat
     8341 tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc atcataccat actccgtaac
     8401 aaattacctg gaggatggta tcgcaaggaa acgaaccgca cgggcaatgt cacgggtgcg
     8461 tggcgcgggc ggcagactgg cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt
     8521 gtcacaaatt accaaaaccc cgcgatcgtc ggcgtcgcga atcagtcgcc cgactccctg
     8581 cttgagagta atcaccgcat ccggcagttg tacttcatcg aacgggtcgc caccgcgcaa
     8641 acggcaatct tccatgcgcg cttttaacag tggatcatca ggcgaggtaa acggcaattt
     8701 gtcgataatc accaatgaca atgtatcgcc acgcacgtcc accccctccc agaaactact
     8761 ggttgccaca agaagcgcgt taccggcact gacaaattgc tgcaacagtt gccctttgct
     8821 ggtttccccc tgcaataata cgggaagcgt catggtagcg cggaactgct cggccagatc
     8881 gcgcatcatg gcgtgcgagg tacaaagcat aaaacagcga ccgttgttag cttcgatgat
     8941 cgggcgcagc atagccgcca gttgccgcgc ggaacccggt tgattggttt gcggcaggtt
     9001 gcgcggcaca cagagtaacg cctggcagct gtaatcaaac gggcttggca atagcaacga
     9061 ctccgcctgt tctatcccaa gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag
     9121 cgttgctgag gtgaagatcc agctaccggg cttttgcgcc attaactctt tgaatttatc
     9181 cgccacgctg agcggcgtga gagccagagt aaaatggcgc gaagtgcatt cgtaccagta
     9241 gctgtagccc ggctgattga tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc
     9301 gcgctcaaat gccgcatcca gcaaggcgga acgccccagt gacagtttcg ccacgtcata
     9361 acaaagttcc agggtgtcat cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa
     9421 cagctcacgc aggttaccgc gataacctgg ttcaccgagt tgcagacgaa aatcctgcgc
     9481 actctgggca agacgatcag cgcacttttg taactgctgg gtgtctttta attcggtgcg
     9541 gtaagcgatg gtgatgtctt ttgccaggtc gagcagttgt cgactggaga gtgactgacc
     9601 aaaatactgg ctggcaatgt ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc
     9661 ttccgggatc aactcgccaa atccactctc tttaaccacc atatccgcca gaaagagatg
     9721 atggtttacc accaccacat cggcgtccat cgctttttta cgtgctttga ccacaaagca
     9781 atctttatac atcgggcagt cgctgccaag acagttgtcg ttggtgctgg taaccagcgg
     9841 ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg atatcaccat cgactgtttg
     9901 attagaccag gagcgcagca ggatcacatc gcttaagatt tgtaccggca gatcgccccc
     9961 cgccagcgcc tgctgttcga gacgttcgag gcagaggtag tttgagcgcc ctttcagcag
    10021 cgccacgttg cccgtatatt tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag
    10081 ctgatcctgc aacgcttttg agccggtcga gataatgact ttctttttcg cccgcagcgc
    10141 aggagccagg taagcgtagg ttttgcccgt accggttcct gcttccacca ccagcggctg
    10201 gcctttttct atcgcctggg tgacggctac cgccatctgt cgctgtggtt ctcgcggctt
    10261 aaagcctggt atcgctttcg ccagctgacc gtctggtgca aaatcgtccg tcacactacc
    10321 ccctgttgat ttgaacaggg attatgtcag gatgagggcg ctttcgccag ttgaagtggt
    10381 gacggcgacc tcacattgtg gcagtctttg cagcacgaaa atggaaggat aatgaggaca
    10441 aaatgatgac tatcgttcgt atcgatgctg aagcccgctg gtctgatgtg gtaatccaca
    10501 acaacacgct ctactacact ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa
    10561 ccgccaacac gctggcgcag attgatgccg tgctggaaaa acagggcagc aataaatcga
    10621 gcattctgga tgccaccatt ttcctggccg ataaaaacga cttcgcggcg atgaataaag
    10681 cgtgggatgc ctgggttgtc gcgggtcatg cgccggtgcg ctgcacggta caagcgggtt
    10741 tgatgaaccc gaagtataaa gttgaaatta agattgtggc tgcggtgtaa gctttatcga
    10801 agcaaaataa gtcagacgat aatttatcga taatactggt cggttttaca taaatcgacc
    10861 agagattaag atattacctg ttgatgctcc tgctaattca ttatttattt ttataatttc
    10921 aattttatct ataaaactaa ctatataaat aaccataacc atgccgttgt gttcgccctc
    10981 tgactagcaa ctctggtttt ctttcttagc tgaataatct ggttatagcg tgaggactga
    11041 ccgttaaatt tctccatctt actggataac acaccatgag ctgctacctt taaaacacct
    11101 gaatgtcaac aggttaactc gtgccatatc gttataatcc ctttgatatt agatgcaaat
    11161 taaggtcata tatagtctta tcgataacat ggttaatttt aaggataaga atatgcctgc
    11221 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
    11281 ttcgatgaat gaaagcacag cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
    11341 cgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggttcac
    11401 ggaaaacatg gttggatggg caaaaaaaat ggagtctggt gaacgcactg taattaaaaa
    11461 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagcttaatt
    11521 tatatcagca taaatgggtc aggacgcttt taatcacata taaaaagcgt cctttttctc
    11581 acaatcaaat gatcatcggc ttctataacg aaatcgatac ggattagtgt gttactcgtc
    11641 ttcatcctca aaacgcgcca cgattcgctc gccggagtgg ttggcacgca gctcttctgc
    11701 caccagcgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
    11761 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
    11821 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
    11881 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
    11941 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
    12001 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
    12061 tttcggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
    12121 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
    12181 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
    12241 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
    12301 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
    12361 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
    12421 gctcacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
    12481 ggtacaggaa tatctgcaca gcggtgattg ctatcaggtg aatctcgccc agcgttttca
    12541 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
    12601 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
    12661 gtttattctt tgtgataata gtgaaatcca gacccgcccg attaaaggca cgctaccacg
    12721 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
    12781 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
    12841 agccggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
    12901 tctggtcagc actataacgg cgcgactacc agaacagtta cacgccagcg atctgctgcg
    12961 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
    13021 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
    13081 ttgcggcaac atggatacca gcattactat ccgcacgctg actgccatta acggacaaat
    13141 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
    13201 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
    13261 tagcctgacg cttgatgatt ttttatctcg ctttcaactt ttgcgcccgc aaattaaccg
    13321 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
    13381 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
    13441 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
    13501 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
    13561 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
    13621 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
    13681 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
    13741 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
    13801 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
    13861 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
    13921 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
    13981 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
    14041 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcgggta aacagttcgt
    14101 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
    14161 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
    14221 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
    14281 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
    14341 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
    14401 cgcctataac ggcaatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
    14461 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
    14521 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
    14581 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
    14641 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
    14701 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
    14761 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
    14821 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
    14881 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
    14941 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
    15001 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
    15061 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
    15121 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
    15181 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
    15241 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
    15301 aaccggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
    15361 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
    15421 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
    15481 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
    15541 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
    15601 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
    15661 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
    15721 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
    15781 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg cacactctat tgctccagca
    15841 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
    15901 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
    15961 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
    16021 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
    16081 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
    16141 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
    16201 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
    16261 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
    16321 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
    16381 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
    16441 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
    16501 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
    16561 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
    16621 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
    16681 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
    16741 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
    16801 tacgtcctga cgtgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
    16861 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
    16921 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
    16981 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
    17041 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
    17101 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
    17161 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
    17221 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
    17281 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
    17341 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
    17401 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
    17461 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
    17521 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
    17581 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
    17641 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
    17701 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
    17761 ccgcgcagag aacgcgacgc cagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
    17821 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
    17881 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
    17941 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
    18001 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
    18061 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
    18121 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
    18181 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
    18241 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
    18301 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
    18361 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
    18421 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
    18481 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
    18541 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
    18601 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
    18661 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
    18721 cgacaaaatg caaacgaaag gtgcggcata gagagccaga aaggtaggcg ttaaaaggcc
    18781 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
    18841 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
    18901 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
    18961 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
    19021 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
    19081 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
    19141 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
    19201 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
    19261 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
    19321 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
    19381 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggccc
    19441 gtgatgatga cccaagcttt gatgaactgg tggcactggc agtagaaaca ggccgtgaag
    19501 gcgtgaaagc tctgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
    19561 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
    19621 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
    19681 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
    19741 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
    19801 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
    19861 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
    19921 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
    19981 aaaaagatat cgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
    20041 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
    20101 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
    20161 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
    20221 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
    20281 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
    20341 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
    20401 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
    20461 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
    20521 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca
    20581 cgtttcttct ctgttcctgc aagcaatgcg tgtggcaatt cctgccgtca tcgtggcgct
    20641 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
    20701 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
    20761 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
    20821 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
    20881 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
    20941 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
    21001 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
    21061 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
    21121 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    21181 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    21241 ggaagaacag cgtgctaatg gcgcagagat cgatgacggt gctatcaacg gtatcaaagt
    21301 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    21361 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    21421 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    21481 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    21541 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    21601 tgtcaacatc ccgctggtgg tttcgaccat caccgggcag gatggacaga cccgtgtcac
    21661 caccgtgcaa accattcttg accagttaat gccaggcctg gtaccactgc tgctgacctt
    21721 tgcttgtatg tggctgctgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    21781 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    21841 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    21901 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    21961 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    22021 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    22081 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    22141 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    22201 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    22261 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    22321 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tatttttaat
    22381 aatcagacat agcttaggct atattacctc ttcccttatt tgttatttat tttaacgttt
    22441 cattgatata taaatctaaa tgtaaatcgt tatcaataaa gcaatgaaat aatatattcc
    22501 aacagttgtt ttatattctc aaaatatgtt aaggttgcgc cctcatttgg ggagtagccg
    22561 atttccagat tccggaaatg tacgtgtcaa catactcgtt gcaaaacgtg gcacgtacgg
    22621 actgaatact ttcagtcagg cgagaccata tgcacatcaa tcgctatgcc tgcatgaacg
    22681 cgaaaaccat cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga tgatgtgttt
    22741 tatggatacc ccggtcagga cattgtcatg aatatcactg ctactgttct tcttgcgttt
    22801 ggtatgtcga tggatgcatt tgctgcatca atcggtaaag gtgccaccct ccataaaccg
    22861 aaattttctg aagcattgcg aaccggcctt atttttggtg ccgtcgaaac cctgacgccg
    22921 ctgatcggct ggggaatggg catgttagcc agccggtttg tccttgaatg gaaccactgg
    22981 attgcgtttg tgctgctgat attcctcggc gggcgaatga ttattgaggg ttttcgtggc
    23041 gcagatgatg aagatgaaga gccgcgccgt cggcacggtt tctggctact ggtaaccacc
    23101 gcgattgcca ccagcctgga tgccatggct gtgggtgttg gtcttgcttt cctgcaggtc
    23161 aacattatcg cgaccgcatt ggccattggt tgtgcaacct tgattatgtc gacattaggg
    23221 atgatggttg gtcgctttat cggctcaatt attgggaaaa aagcggaaat tctcggcggg
    23281 ctggtgctga tcggcatcgg cgtccagatc ctctggacgc acttccacgg ttaataagaa
    23341 cgctgccaga ggtgaatatt aaagtccgtc tggcagtcga acacttcttt tgctgccagt
    23401 gtttgccaga cttctggctt cgcacgccag gcaaacggcg tcatctgcaa taatgcgacg
    23461 gcttcatcac cgcgaagacg catcggataa cacaactcat cactctgctg taatgtaaaa
    23521 ccttccagtt gttctgcatg aggtgcatga agatgtactt cattgtaaat cagccccttc
    23581 agctccatca aatgtcgcgg tcccggcgtg gcagtaatga cccagccgcc gggcttcact
    23641 actcgtgcta attcttctgc tttacacggc gcgtaaatac gtattatggc gtccatactg
    23701 gtatcggaaa acggcaaacg gtggctggaa gcgacacaaa gagtgacctg cggatagcgt
    23761 ttcgccgccg cttttatcgc taccttcgag acatccaggc caaacgtggt gatttcgggc
    23821 aacacatcgg caaatgcgtg tgtgtaatac ccttcaccac agccaatatc cagcaccgcc
    23881 gtggcctttt catcaagccg ttcccgcagt tgggcgacaa ttgcatcacg cagcggctga
    23941 taatgtccgg catctaagaa tgcgcggcgt gcttgtatca tttccgcgct gtcgcccgga
    24001 tcacgagacc gtttatgctg aacgggcagc agattgacat acccttcttt cgccatatca
    24061 aactgatgtc gctggggaca gatatagctg tttttttcac gcgaaagagg ctgatggcaa
    24121 agtggacagg aaaacgacat gacaactccg gcaggatact aaaggccgca agtgtaacgc
    24181 gaatcacgcg gcgggcaaat atccgaacgc caggagcgaa gataaagcat agtaaaaagc
    24241 ctcgcattcg acgaggcttt atagcgagat tgaagcgtat tcacacttca gatcagtgga
    24301 ttcgatcaga tagctgttac gttaacagct gccggacctt tctggccgtc ctgaatttcg
    24361 aactcaacgt tctgaccttc agccagagtt ttgaagccat taccctggat agcggagaag
    24421 tgtacgaaca catctttgct gccatcagcc ggagtaatga agccaaaacc tttagactcg
    24481 ttgaaccact taacctgacc tttaatcttt gccatttgaa aaattcctta gattgttttc
    24541 ttcgcccgca ggcataacat agataaaact gacacattac tgcatgaggc accaatataa
    24601 ggctcggcag agaagcagta ttcaacgtca acgtgtttac tcaggacttc tttactgaaa
    24661 atgccacaca taaacagaac tgtacctcgt ttaacccgaa atctgttatc acatacaacg
    24721 ttaattatgg caagccattt ttaaacatgt ctcgatcaga cgcacaaatc tcgatacgtc
    24781 ttccactttt tttgcacact tatgcaacgg aatacgcgcc gatatatcat tgtgcttaac
    24841 cttgccagtt caggcagata cttaaactgg cgtattttct aacatagttc aataagttca
    24901 gttgttccag gccagtggga gaagttatta catagtgcgt gcaatatcac attttttgct
    24961 atgcaatgaa taaaaagtta tatcactttt tctcataaaa cagtcagtta acggctatca
    25021 attaccctaa agagaagtca atccccaaag ggattgaaaa tttaaaataa gaaaaattga
    25081 tgaatgagca aaaaatcaag agagaaacgt ttcgctaata aataatacca cgcttaatca
    25141 caaatccgat gtaaacatcc taataaatta atgaggaaaa tatctttttc tgatatcccg
    25201 ttacgcaaat aaataatatg ataattattt gaagtcagac cagagtttta atttgaagca
    25261 gttgcaccat ggcagtgcgt ataatcagga tgaattgagt aacccttcaa cgacggggtt
    25321 actcaagatt gaggaaggat tatcgttcgg cgaccagacg aataatatct tcgtcttcaa
    25381 ccgatttttg actctcaacg gtctttttat ctgctttctc aggttcgttt gcttcgcaca
    25441 aacgacgtaa cagtgcattt tgacgttttt gctgatccag caacgcctca agcagttcaa
    25501 tctgttcgtt agtccgagaa ctggcacgat tgataaaaaa ccacaagatg agcccgataa
    25561 gaagaaccac caccgataca accaaagacg caatgttcat cacgcctgaa tttacaactt
    25621 cgttcatttc accacctctg agtagaggcg ctattctacc actgctggag aggaagaaaa
    25681 tctagtgctg aaaaaatgat atcaccacgg gataaactgg ttaatggcac aaattccgct
    25741 gaaaaattgt acatcctgat cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca
    25801 agccaacacc acgacaacca gaacgaccca tcgaaacttt ttcactccaa tctccgcttc
    25861 acctacctta tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac tatgtcgata
    25921 ttcgtgcttt ttcggaatga gtagcttgct tcagcgcata ttgccgctac gattaagcga
    25981 acataaaaag agaagaggtt gtaatgcgat tcatcattcg cacagttatg ctgatagcac
    26041 tggtatggat aggattatta ctcagcggat atggcgtttt gattggaagc aaagagaatg
    26101 ccgcaggatt aggtttgcaa tgtacctatc tgactgccag aggcaccagc acggtacaat
    26161 acttgcacac taaaagtggt ttcttgggga taacagattg cccactgtta cgtaaaagca
    26221 atatcgtggt cgataatggt taagggtaat aaataaccag attctcactc atgagtaaaa
    26281 tattcttata gcaagcgcgt ttaatataac gcaccttcaa ctttattttt ctcatgctaa
    26341 ataaagaacc tgtctgtttg atattccgac aggttcttac tctcatcaga acggatagtc
    26401 gtgataaccc atttgggcag aaattttgcg cgctgcggta tgcaacattg cgacatattc
    26461 ctgtaaacgc tcttcagaga aacgcaacgt cgggaaggag atgctcaaac cggcaatgac
    26521 cacgccaaag cgatcaaata ccggtaccgc aatgcatcgc agcccttctt cctgctcttc
    26581 gttatcttcg ccatacccct gctcgcgcac ctggtccaga acgggtaata acgcttctgt
    26641 actggtgatg gtccgctcgg tactgcgttt atactccacg ccctcaagaa tttgcttcac
    26701 ttcatcgaga tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt acagcggatt
    26761 acgacgccca atccgtgaat acatgcgcaa attgtacata gagtcaattt tgtgaatgta
    26821 aacaatactg tcttcgtcca gtgcgccgag gtggatagtt tctttggtca ggcgggagat
    26881 ctcacgcatc tggatatctg cgctacgaat taaatcgacg ttttgtaacg cgcgagcgcc
    26941 cagttcaaac aatttcaggg tcagggaata tttctccgac tccccttcct gcgccacata
    27001 acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg
    27061 ctgcgacagc tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa
    27121 aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt tagccattta
    27181 tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa tttcgcacca
    27241 gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc accgtgaata
    27301 tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt cctatgccaa
    27361 aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta accattgtga
    27421 ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccctg ccaacaatcg
    27481 ccacggacct tcatgccacg ccagccagtt ccatctgggt agtgaacgcc tatcaaatcg
    27541 ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc tatcgacgta
    27601 tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc ctttctgatt
    27661 cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga
    27721 gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggg agagggatgg
    27781 gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa
    27841 tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgtta ggtattatcg
    27901 ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt aaaccccgtt
    27961 tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatc actgcgttga
    28021 gtggtttcgc tcaggggcaa tcgctgacgt taattgctgc ggaactggtg gtaatggttg
    28081 ttgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg
    28141 atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc tctttctgcg
    28201 cacaaatgct ggcaatggtt tccctgccct tttacctgca aaccgtgctc gggcgtagtg
    28261 aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacgatggtg atggctccgt
    28321 tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgctcta gggttgttca
    28381 tcatggctgc ggggcttttt tccctggttc tgctgcccgc gtcacctgcg gatatcaata
    28441 ttatctggcc gatgatctta tgtggcgctg gatttggctt gttccagtca cccaataacc
    28501 acaccattat tacctccgct cctcgcgaac gtagcggtgg agccagtggc atgttaggaa
    28561 cggctcgtct actgggtcag agtagcggcg cggcactggt ggcgctgatg ctaaatcagt
    28621 tcggtgataa tggtacgcac gtttcgctga tggctgcggc tattctggca gtgattgctg
    28681 cctgtgtcag tggtttacgt atcactcagc cacgatccag ggcataaaaa aagcgcgtcg
    28741 atcaggacgc gctttttagt atttacttca tattacttca ggtattcacc cgtacgcaga
    28801 gcttcaattc gtttatccag cggcgggtga gtcatgaaca actcactgag cgatttcgac
    28861 ttaccgttaa tgcagaaagc catcatgctg gttgcttctt gcggttcata gctggttttc
    28921 aggcgctgca gcgcggcaat cattttctcg cgaccaacca gttttgccga accggcatca
    28981 gcatggaatt cacgatgacg cgagaaccac atggtgataa tgctcgccag aataccaaac
    29041 accagttcca gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt gctctcttca
    29101 ccttcatcac gatttccgcc cataaaaccc gcggcaagct gcgccagaat acgggaaata
    29161 aagataacga aggtgttcac cacgccctga atcagcgtca tggtgaccat atcaccattg
    29221 gcgatgtggc tgatttcgtg agcaattacc gcctcggctt catccgggct catgttctgc
    29281 agcaaaccgg tgctgacagc aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg
    29341 ttgatgtccg gcgcatggta gatagccact tgcggcatag cgatccctgc ctgacgagcc
    29401 tgggttgcta cagtattaac cagccaacgt tctctttcgt tacgcggttg ctcgatcact
    29461 tccccgccaa cagatcgtaa tgccatccat ttggacatca gaagcgaaac gaaggaacca
    29521 ccaaaaccga acagcaaggc catgatcatc agcccctgaa cgctgctcga ctgtatccct
    29581 gtcaggctca gtaccagccc gaaaacgacc attacggcca ggttcgttag caggaagagc
    29641 gcgattcgca tcataatttt ctttttacct cagtttaaca aaacgcaata tgcgataccc
    29701 acatcgtatg ggttacgcga ctattttcaa gtctggatag tgcgtaagtc accagaaaga
    29761 cacaacttta cattttgtag catctgattt acggcatctt gccgctgtta aaaaatcagg
    29821 cacaatttct tgtgcctgat tgatattact tgacgggagc gggttgttcc gcgggtctgg
    29881 ctttttcaag cttcgccaga tcgagtgcga tattcaccgt ctcatccaga taaggatccg
    29941 gctcctggta atctttcggt agatcatcca gcttcttcaa ctccggttta ccttcgcgtt
    30001 taaagcgttc gttcaaacgc gccagacgcg tcgcatcatc ttcattattc tctttctcac
    30061 gcacagcgta gttcagagaa acgatattgc gcttgtcctt catagcgttg aagcgcgcga
    30121 tatccttcat gatgttctgg aactcaggat ctttcgcgat acgcgcatta tgttccttca
    30181 gcagctccgg cccaaaggcc gttaaatcgc ctgatttcac ataagtcgcg gcatcaatgc
    30241 tatcccacgg cagcgcgtta tcttcgaatt tctcacccgt ttccgtttct tcattacccg
    30301 tcggcatgat gatgtctggc gttacgcctt tacgttgcgt actgccgccg ttaacgcgat
    30361 agaatttctg gatcgtgtac tgcacagaac ccagcgctgg ccattcagga cgtaacatct
    30421 gatcgtaaat acggttcaat gaacggtatt gctgaacggt gcctttacca aacgtcggtt
    30481 cacccacaac cagcgcacga ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg
    30541 aagcactgaa acggtcaacc agtaccacca gcgggccttt atagaacacc tgcccgtcgg
    30601 tatcgctgtc ttcacgaacc ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag
    30661 gaataaacag acctgagagc gataccgctt cggtcaacgc cccaccgcca ttgctacgca
    30721 ggtcgatgat cacactgctg acattctgtt tttccagttt ctgcagttga actttgacat
    30781 cgtctgtcaa acccacatag aagcccggaa tatccagcac gccgactttc tctttaccga
    30841 cggtcttcac cgacatttta accgcgcggt cttcgagacg aatacgttca cgggtcaatg
    30901 ttacagtgcg ggtcttggtc cctttaccag caggtaaaat ttccagacga actttactgc
    30961 ccttcggccc tttaattaag gcaaccacat catcaagacg ccagccaatc acgtcaacca
    31021 tcggcttgcc tgtttgacca acaccgacaa ttttgtcacc aacgctgata gctttactct
    31081 tcgctgccgg accacctgcc accatcgaat tgataacggt gtagtcatca tccatttgca
    31141 gcactgcgcc aataccttcc agcgacaaac tcatttcagt gttgaactgt tcggtattac
    31201 gcggggaaag atagttggta tgcgggtcga tttcacgcgc aaacgccgtc attgccagcg
    31261 agaaaacatc ttcgctgttg gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg
    31321 tcagggtttc acgaatttct ttatccgttt ttcctgccag cttcaggctt aactcgtcga
    31381 atttgacttt actgtcccac agcgcgttca actcagcctc gtttttcggc cagggcgctt
    31441 tgctgcggtc aaggttataa gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg
    31501 acaaagcgta ctggtaacgc tcaaaacggc gcttttgcgc cagattgtag agatcgtaga
    31561 aaacgtcgag tttgcctgaa cgcagttcat cgcctaactc ggttttcttt ttcgcgaact
    31621 gttcaacatc gcttgccagc agcacgttgt ggctgtaatc gagcagattc aggtagcggt
    31681 caaagatttt ggccgaaaat gcctgatcga ggtcgaactg gcgataatga gaacgggtga
    31741 agcgcgacgt tacgcgctca cttaccgtcg catgctgcgt ctcttccttt aataccggaa
    31801 tttgatcagc acgcgtgata tcttctacag cgaaggtctg gcctgctatt gcaagcaggc
    31861 cagctaacgc ggtaagccta aaaaacatgt tcatgcctgg cccggcctcc gtttcagaac
    31921 accaggtgtt ctgcgcgcac aatcaaagac atacccgaat tcagctggac gcggacgccg
    31981 tctttggtga tttctaatac ggtggcatcc atcgcgtttt gacccgcttt caccttcagg
    32041 gcttgtccga cagtcagagc tgaaatgtca gaaaccgggg tgtgctgttc ttcgcgaggt
    32101 gcttttactg tttttggcgc tttctctacc ggcttttgcg cacgaggttt acgttcagcg
    32161 ccttctttgc ggcgtggcgt ggtcggacgt ggcttgcgtt cgcggcgcgg tgcgtcttct
    32221 ttctcaccag cagctgcggc agcttcgcgt tttttcgctt gctgttcagc acgctgtgcc
    32281 tgaacacgcg ctttcgcttc ttcaagctgc ttgcgagcat gctctacatg ttgctcgtcc
    32341 agctcaccgc atgggttgcc gtcaagatcg acacgcgttg cgccgggttt aacaccgtaa
    32401 agataacgcc agctcgaagt gtagagacgt aaagcggatc gtaattgcgt tttgctcagg
    32461 ttcatttccc cggcaacacg atcgaccaaa tcctgaaaaa taccgatttt cagcggacgc
    32521 gcttcacctt ccgcactgaa acagtgggga aaacgttcgg ccagaaacgc gattacttct
    32581 ttactgctat tcaacttagg ttgattttcc atgaaatttc ctgattacaa cggacgtagc
    32641 caacaagcgc aggcatgaac aggcgtcatt ataatgacgc taccagtaaa tgctacgtta
    32701 tccgttgatt atcctgcgac gctcgcaaag aattttttgt aatccgtcgt tgcaagcact
    32761 ttttcaagct gtgccacaag ctgacgtaag ccttgctcgt cctcgtctgt aaagcgaccg
    32821 aagacggtac tatcgatgtc aagaacacca ataatctgat ttttcaccac cagcggcaga
    32881 acaatttcag aattactcgc cgcatcacag gcaatatgcc cgtcaaacgc gtgaacatcc
    32941 tcgatacgct gcacttgatt gcgggcaacc gcagtgccgc acaccccgcg cccgacgggt
    33001 atccggacac aggcaatttt gccctgaaat ggtcctagta ccagtgtatc gtcctcaagc
    33061 aaataaaaac ctgcccagtt tacgtcagtg agacgctcgt ataacaacgc actggtgttc
    33121 gcaagcgttg ccagaaaact ggtttctccc gccatcagcg cgttaaagtc gcgatttaaa
    33181 tccgcgtaaa attctgtttt gttcattata taatcactta gttgtcttac ctggaactgc
    33241 cagcctatta aaataagcat taaatgcgtt aatgctcaag atcattccaa tcatgggtta
    33301 atattaatgt taattcttat tacatttggc acgtcatggc tcttaacaca ccacaaatta
    33361 cgccgacaaa aaagataaca gtgagggcaa tcggcgagga actgccgcgt ggtgattacc
    33421 aacgttgccc gcaatgtgac atgctgttta gcctgcccga gataaattct catcagagtg
    33481 cctattgtcc gcgctgtcag gcaaaaattc gcgacgggcg cgactggtcg ctaacgcgcc
    33541 tggcggcaat ggctttcacc atgctgttgc tgatgccgtt tgcctggggc gaaccgctgt
    33601 tgcatatctg gctgttaggt attcgtatcg acgccaacgt catgcaaggc atctggcaaa
    33661 tgaccaaaca gggcgatacg ataacggggg cgatggtctt tttctgcgtt attggtgccc
    33721 ccctcattct ggtgtcctcc atagcatatt tatggtttgg taaccgactg ggaatgaatt
    33781 tacgtccggt actgctgatg cttgagcgac ttaaagagtg ggtaatgctg gatatctacc
    33841 tggtcggcat tggcgttgct tctataaagg tacaggatta tgcccatatc caggcgggtg
    33901 tcggcttgtt ctcttttgtg gcgttggtga ttttaacgac ggtgacgttg tcacatctta
    33961 atgtcgagga gctgtgggag cgattttatc cgcagcgccc cgctacgcgt agggacgaga
    34021 aacttcgtgt ctgtcttggg tgccatttta ccggctatcc tgatcagcgt ggtcgctgcc
    34081 cgcgttgcca tatcccgcta cgcctgcgtc gccgtcatag tttgcaaaaa tgctgggcgg
    34141 cgctgttagc gtcaatcgtt ttgttgttac ctgccaacct gttgcctatt tctatcattt
    34201 atctgaacgg aggacggcag gaagatacaa ttctttccgg aattatgtcg ctggcaagta
    34261 gcaacattgc ggttgcggga atcgtgttta tcgccagtat tctggtaccg tttactaaag
    34321 tgatcgtcat gttcacttta ctgttgagca ttcattttaa atgccagcaa ggtttacgca
    34381 cacgcattct gttactgcgg atggtgacct ggattggtcg ctggtcgatg ctcgacctgt
    34441 ttgtcatatc tttaaccatg tcgctgatta atcgcgatca gatcctcgct tttactatgg
    34501 gaccggctgc gttttatttc ggcgcagcgg taattttgac tattcttgct gtggaatggc
    34561 tggacagccg cttactttgg gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa
    34621 gcgcagatta aaaataaacg ccgtatctca cctttctggc tgctgccgtt catcgcgcta
    34681 atgattgccg gttggctgat ttgggacagt tatcaggacc ggggtaatac cgtcaccatc
    34741 gactttatgt cggcggatgg tattgtcccg ggccgtacgc ctgttcgtta tcagggcgtt
    34801 gaagtcggaa cagtgcagga tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag
    34861 gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag agactcagtt ctggctggtg
    34921 acgccaaaag catcgttggc aggtgtctcc gggctggacg ccctcgtcgg tggtaactat
    34981 atcggcatga tgccaggtaa aggtaaagag caggatcact ttgtcgcact cgatacccaa
    35041 ccgaaatatc gactggacaa tggcgatctg atgatccacc tgcaagcccc cgatctcggt
    35101 tcgctgaaca gcggttcatt ggtctatttc cgcaagatcc ctgtgggaaa agtctacgac
    35161 tatgccatca atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt
    35221 accgatctgg tgaaaaaagg tagccgtttc tggaacgttt ccggcgttga tgccaatgtc
    35281 agtatcagtg gcgcgaaggt gaaactggaa agtctggcgg cactggttaa cggtgcgatt
    35341 gccttcgatt cacctgaaga gtcgaaacct gccgaggcgg aagatacctt tggtctgtat
    35401 gaagatctgg cccacagcca gcgtggcgta ataataaaac tggaactgcc gagtggggca
    35461 ggattaaccg ccgactcgac gccattaatg tatcagggac tggaagtcgg acagctaact
    35521 aaactggatt taaatcctgg tggtaaagtc actggggaaa tgacagttga tcccagcgtc
    35581 gttaccctgc ttcgtgaaaa tacccgcatc gaattacgta atccgaaatt atcccttagc
    35641 gatgccaatc tcagcgccct gctgactggc aaaaccttcg agttggtacc cggcgatggc
    35701 gagccacgca aagagttcgt tgttgtgcca ggcgaaaaag cactgctgca tgaacctgat
    35761 gttctgacgc tgaccctgac cgcgccggaa agttacggta ttgatgctgg tcagccgctc
    35821 attcttcacg gcgtgcaggt aggccaggtt atcgatcgta aactcaccag caaaggcgtc
    35881 acctttaccg tcgccatcga gcctcagcat cgagaactgg taaaaggcga tagcaaattt
    35941 gtcgtcaaca gccgtgtcga cgtgaaggtg gggctggatg gcgttgagtt tctcggtgcc
    36001 agcgcctcag aatggattaa cggcgggata cgtattctgc cgggcgataa aggcgagatg
    36061 aaagccagct atccactgta tgccaatctg gaaaaagcgc tggagaacag ccttagcgat
    36121 ttacccacca caaccgtgag tttgagtgca gagacgctgc cggatgtgca ggcaggatcg
    36181 gtagtgctgt accgtaaatt tgaagttggt gaagttatta ccgtgcgtcc gcgagctaac
    36241 gcgtttgata tcgatctgca tattaagccg gagtatcgca accttctgac cagcaatagc
    36301 gtgttctggg cagaaggcgg ggcgaaagtt cagctgaatg gtagtggcct gaccgtacag
    36361 gcatccccgc tctccagagc attaaaaggg gctattagct tcgataacct cagtggtgcc
    36421 agcgccagtc agcgtaaagg cgacaaacgt attctgtatg cttccgaaac agcggcccgt
    36481 gcggttggtg ggcagattac gcttcacgct ttcgatgccg gaaaactggc ggtcgggatg
    36541 ccaattcgct atctcggtat tgatatcggg caaatccaga cgctggatct gattaccgcg
    36601 cgcaatgaag tacaggcaaa ggcggtgctc tatccggaat atgtccagac ctttgctcgc
    36661 ggtggtacgc gcttctcagt ggtcacaccg caaatttcag ccgcgggcgt tgagcatctt
    36721 gatactatcc tccagccgta tatcaacgtc gaaccaggcc ggggcaatcc tcgccgcgac
    36781 tttgaattac aagaggccac cattactgat tcgcgttacc tggatggctt aagcattatt
    36841 gttgaagcgc cggaagccgg ttcgttaggt attggtacgc ctgtgctgtt ccgtggtctg
    36901 gaagtcggta cggttacagg aatgacgctg gggacattgt cagatcgcgt gatgattgcg
    36961 atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtctt ctggttggca
    37021 tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg cacctttaac
    37081 cagtttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact ggcaccgaaa
    37141 gcccaggaag gcaaacactt cctgttgcag gaaagtgaac cgaaagagtg gcgtgaatgg
    37201 ggaactgcgc ttcccaaata atgcccactg ctccggcgtg cctgcgccgg agcgtttatg
    37261 ctaaactgcg cgcctgtttt tttgccagtg gtacatgctc gtggcccaac acaccgttta
    37321 tttcccggac gcctttctga cacaaatgcg cgaagcgatg ccttcgacgc tctcatttga
    37381 tgattttctt gccgcctgtc agcgcccgtt gcgccgcagc attcgcgtta atacgctgaa
    37441 aatctccgtt gctgatttcc tgcaattaac cgctccttat ggctggacgc ttacgccaat
    37501 tccgtggtgt gaagaaggtt tctggattga acgcgacaat gaagatgcat tgccattggg
    37561 tagtaccgcc gagcatttaa gcggcctgtt ttatattcag gaagccagtt caatgttgcc
    37621 cgttgccgcc ttgtttgctg acgataatgc accacagcgg gtgatggatg tcgcagctgc
    37681 gccaggctcc aaaacgacgc aaattgccgc gcggatgaat aacgaagggg caatccttgc
    37741 caatgagttt tccgccagtc gggtaaaagt gttacatgcc aatatcagcc gctgtggcat
    37801 cagtaatgtt gcgctcacac attttgatgg ccgcgtgttt ggtgcggcag tgccagaaat
    37861 gttcgatgcc attttgctgg acgctccctg ctctggcgaa ggcgtggtgc gtaaagatcc
    37921 cgatgcgcta aaaaactggt caccagaaag caatcaggaa atcgcagcta cacaacggga
    37981 gcttatcgac agcgcctttc atgcattacg tcctggtggt acgctggttt actcgacctg
    38041 taccttaaac caggaagaaa acgaagccgt ttgcctgtgg ctgaaagaga cttaccccga
    38101 cgcagtagag tttttaccac ttggcgatct cttccctggt gcaaacaaag cgctgaccga
    38161 agaaggcttt ttgcatgttt tcccacaaat ttacgactgc gaaggcttct tcgttgctcg
    38221 tctgcgtaaa actcaggcga tccccgcctt acccgccccc aaatacaaag tgggcaattt
    38281 cccgttcagc ccggtgaaag atcgcgaagc cggacaaatt cgtcaggcgg ctgcaagtgt
    38341 tggcttaaac tgggatggaa acctgcgcct ctggcaacgc gacaaagaac tgtggttgtt
    38401 cccggtgggc attgaagccc tgatcggtaa agtccgattt tctcgcttgg ggattaaact
    38461 tgccgaaacg cacaacaaag gttatcgctg gcagcatgaa gcagttattg cccttgccac
    38521 ccccgacaat gtgaacgctt ttgaactgac accgcaggaa gcggaggagt ggtatcgcgg
    38581 gcgcgatgtt tacccgcaag ccgcgccagt ggcggatgac gtgttggtta ctttccagca
    38641 tcaaccgatt ggtttagcca aacggattgg ttcgcgattg aaaaacagct atccgcgtga
    38701 actggtgcgc gatgggaaac tttttaccgg taacgcctga cagcgcacaa aaaaagcgca
    38761 ctttttgact ggcacattcg gctgcctcaa ctaggctgaa aaatggtgcg atcggactgg
    38821 tcgtaccaca accggcagct aaatggagag cacgaagatg aaaaccagtg tgcgcatagg
    38881 cgcttttgaa atcgacgacg gcgaattaca cggtgaatcg ccaggtgatc gaacgttaac
    38941 cattccttgt aaatctgacc ccgatttatg tatgcaactg gatgcctggg atgctgaaac
    39001 cagtatccct gccctgctaa atggcgaaca ctctgtcctt taccgtaccc gttacgatca
    39061 acaatctgat gcctggatta tgcgtcttgc ctgatccaaa aagaacccgt cggcatggcg
    39121 ggttatttgt cctggttatt cccccgttgt aaaatctctc ctaaacttaa cggtacggca
    39181 ccacacttcg gggatgaaat gttcgcgctg gtactttttg tttgctacct ggatggcggt
    39241 tgtgaagata ttgttgtgga tgtctacaac acggaacagc agtgtcttta ttctatgagc
    39301 gatcaacgga tccgccaggg cggttgtttt ccgattgagg attttataga tggtttctgg
    39361 cgacctgcac aggagtacgg tgatttttaa ttattgcaat tgcacaagag tcagttcgcc
    39421 cccaaagaca gcaccggtat caatataatg caggttgcca atatccacgc gatgtcgcaa
    39481 cggtgtatga ccaaaccaga aatgatcagc acctgtaatt ccctgccctt tttggcgttc
    39541 acctaatcgc gagcggctcc acaagacctg atgcaaatca acgtcctttt gccattcata
    39601 aacatcatct ggataatcgg catgagcaat aacatgtttg ccggtacgac tgtgtacttc
    39661 aagaataaag ggcaaatgct gacatttttc cagcgccgtt ttcgcttgtt tctgttgatt
    39721 atctgccagc gcaataaacc agtcgccgcc attcatcaac cacaaagaca tctgctggga
    39781 tgccagcgca tccatcgcca tctgttcatg attgcctctt accgcacgaa cccaatgttg
    39841 ttcgagtaac tgcaggcaac gtaaactttg cggcccacga tcgataacgt ctcccactga
    39901 gataagtaaa tctcgccacg gatcaaaacc acaatgccat aatttgcggc gcaactgctc
    39961 aagacaaccg tgtatatcgc cagaaagcca gatatgtcgc cattgatgac ccgcaattct
    40021 ctgataaacg ggcgcaggct gtttcatcaa tattttcctc ccgcgctaaa gatcacataa
    40081 tcttaacaag aatgttaaaa aacgctggac tcagacagta gagtgtgtgt tatggttgac
    40141 tataaagtca gcgaaggaaa tgcttctggc ttttaacaga taaaaagaga ccgaacacga
    40201 ttcctgtatt cggtccaggg aaatggctct tgggagagag ccgtgcgcta aaagttggca
    40261 ttaatgcagg cttagttgcc ttgcccttta agaatagatg acgacgccag gttttccagt
    40321 ttgcgtgcaa aatggtcaat aaaaagcgcg gtggtcatca gcttaaatgt taaaaaccgc
    40381 ccgttctggt gaaagaactg aggcggtttt tttattggaa atcaaaacgc tagtttaggt
    40441 aattaacaga gtttttcagc tcgttctata aacggtgcca gactcatttt ttcgccggga
    40501 ttgttaggat catcaatctg aatcaccgaa atgggttggg ctttagtctt cccactggca
    40561 acttcctttt gtgcgatatc gtttaaagga tactgcacga gggtacttgg gttgatgaca
    40621 tacaaagcat tacccggtcg gcaagtcagc atcacctctt cgcgattaaa cgcccatttg
    40681 tctttaccca cttcaaaacg gctgacggta atcacctgcg gtgcagccag cgccgctgca
    40741 gaactggtga gtaacagaaa cgccagaata ctttttttca tcattttcgt aatccatcaa
    40801 aaaggttcga gagtcgcaag caggctgatg atcaccagca ctaccgcacc tatcgcccat
    40861 tcgagtttag tcatccacac aaaccatggt gccgcacgat cttcatcctg cctcatgcgc
    40921 ggtacaagaa cataacgatt agccagcgca atgaccacca taatcatcac caggatggct
    40981 ttgagcaata agagttggcc ccagtaagtc gtaagcgtgg ggggaaatcc ggtaatcaac
    41041 aatgcattaa gcacgccgct tgccagtacg ccgatcaccg caaaatgccc gcaccaggaa
    41101 aaacgcatca gcgcctgaat agcctggtgt cgccaacggc ctttgatgag ttgcatacac
    41161 cagagcaccg gcagtaatcc cccaaaccac gccgccgcac aaatcaggtg aatcgcgtga
    41221 ttagtctgat ggattttcgc cgttacccct tcattcagcg tcgcatgccc gattcccgcc
    41281 agcagaataa attgcgcggt agtgagcata aacagcaaac gtggcatatt ccgcggttgc
    41341 ataagggcga cgatcaacgt tacgagggcg agaacaattt gccataacca gacgccacca
    41401 aactgcgttt gcaaaacagc ctgccaaatg tttggcgaaa atacatctgc ccatcccgtt
    41461 cccatcaacc cgccctgaac ggcgagcatt gcagtggcac tgatcaaact ccagacggca
    41521 gcgtgctgtt gtaaacgcag aaaacgcttc gccagcaagc gacgaatcgt taagggggcc
    41581 agccaggcac cgtacattgc gaagccaaag accagcatca gcgaggtgaa atggataaat
    41641 cgcagcgcga tccaggtaaa cgccagcatg atttatttca cgctaaaggt gtagtgtcct
    41701 ttcgttttat gcccgtccac cgaaacaaca tgccagtcaa cgatgtaggt tccgggtttc
    41761 agcgaatcgg ccagtggaac aatcagttgc ttttggtcct gctcatttcg cttcgccggt
    41821 aatgttttaa tattttcgtt ttttggcccc gtgatttttg caccactgaa tcctgtttca
    41881 acaccttccg agaagtttaa agtgattgcc tgcggtgcag ctgtcacttg cgcgtttgcc
    41941 gcaggatact gatgcgttaa atgcgcatgt gcccagacgg aaggtgtcac caacgaagtg
    42001 gtcaggattg ccagcgcgta gcgaagggag cgtgcagttg aagtcatatt atctattcct
    42061 ttttgtaata acttttttac agagcataac cttgtctaat gtctgagtcg aggatcatca
    42121 attccggctt gccatcctgg ctcactctta gtaacttttg cccgcgaatg atgaggagat
    42181 taagaatgct gaagaatctg gctaaactgg atcaaacaga aatggataaa gtgaatgtcg
    42241 atttggcggc ggccggggtg gcatttaaag aacgctacaa tatgccggtg atcgctgaag
    42301 cggttgaacg tgaacagcct gaacatttgc gcagctggtt tcgcgagcgg cttattgccc
    42361 accgtttggc ttcggtcaat ctgtcacgtt taccttacga gcccaaactt aaataaaact
    42421 tatacagagt tacactttct tacataacgc ctgctaaatt atgagtattt tctaaaccgc
    42481 acccataatt tgcagtcatt ttgaaaagga agtcattatg tcgttctgga aagttgcagc
    42541 agcgcaatat gagcctcgta aaacctcact caccgaacag gtagctcatc atctggagtt
    42601 tgtgagggcc gccgccaggc aacaatgtca gctgttagtt tttccatcgc tttctttact
    42661 ggggtgtgat tattcgcgac gtgcccttcc tgccccaccc gatctctcac tgttagaccc
    42721 gctttgttat gccgcaacga cctggcgaat gaccatcatt gccggccttc ccgttgaata
    42781 taacgatcgc tttattcgtg gaattgcggt gttcgcaccg tggcgaaaaa cgcctgggat
    42841 ctaccatcag agtcatggtg catgtctggg ccgtcgttcc agaaccatca cggtggtcga
    42901 tgaacaaccg caaggtatgg atatggaccc gacctgttca ctgtttacta cagggcaatg
    42961 tctgggagaa cccgacctgc tggcgtcggc ccgtcgctta caattttttt cacatcagta
    43021 ctcgattgcc gtgctgatgg caaatgcccg tggtaacagt gcactatggg atgaatatgg
    43081 tcgtctcatc gttcgcgccg atcgtggttc attattgtta gtcggtcagc gttcatcaca
    43141 gggttggcaa ggcgatatca ttccattacg ctaggctttt tcggcctgga gcatgccatg
    43201 ttgcgcatta tcgatacaga aacctgcggt ttgcagggag ggatcgttga gattgcctct
    43261 gttgatgtca ttgacggaaa aatcgtcaac cccatgagcc acctggtgcg ccccgatcgt
    43321 cctattagtc cacaagcgat ggcgattcat cgcatcaccg aagccatggt cgccgataaa
    43381 ccgtggattg aagatgtgat cccacactat tacggtagtg aatggtatgt cgcgcataac
    43441 gccagctttg accgccgcgt actgcctgag atgcccggtg agtggatttg cactatgaaa
    43501 ctggcccgtc gtttgtggcc tgggatcaag tacagcaata tggcgttata taaaacacgc
    43561 aagctcaatg tacagacgcc gccgggcctg catcatcacc gcgcgttgta tgactgttat
    43621 atcaccgccg cgttgcttat cgatattatg aacacctccg gctggacggc agaacagatg
    43681 gccgatatca ccggacgtcc gtcgttgatg acgaccttca cctttggcaa ataccgtggc
    43741 aaagcggttt ccgacgttgc cgaacgcgat ccgggctatc tgcgctggtt atttaataac
    43801 ctggacagca tgagcccgga gctgcgttta acactgaaac attatctgga aaatacttag
    43861 tccgcaggcg tagcgggtaa tgttccctgc gccagcgcga ccagaaaagc atattccatc
    43921 gctacgcctt cgtacgattt aaagcgacca gatttaccgc catggcctga gtccatgtcg
    43981 gtacagagca ataaaagatg gtcatcggtt ttcagctcgc gcaatttagc gacccatttt
    44041 gccggttccc aatattgcac ctgagaatcg tgtaaaccgg tcgttaccag taaatgcgga
    44101 taagcctgtg cggtgacgtt gtcatacggg ctgtagcttt tcatgtactc gtaatattgc
    44161 ggatcctgcg ggttacccca ctcttcaaac tcaccagtgg taagaggaat tgattcatca
    44221 agcatcgttg ttacaacatc aacaaacggt acctgggcga taacgccgtg gaataattcc
    44281 gggcgttgat taattgcaac gcccatcaac atgccccccg cactcccgcc catcgcataa
    44341 caaagcgaag gagagccata gcccagtttt aacaatgcat cgcaggcatc aagataatca
    44401 ttaaacgtat ttttcttctt cagaaattta ccgtcttcgt accattgttg ccccagctca
    44461 ccgccgccgc gaacatggac aatggcgtag acaaatccac gatctaataa actcaagcgg
    44521 ctaaaactga aatcggcatc aatacttgcg ccgtaagaac cgtagccata caccagcagc
    44581 gggttgtgcc ctttgcgaaa atgtttgcga tggtagacca acgaaaccgg aacttcgacg
    44641 ccatcacggg cgactatcca caggtgttca ctgcggtaat tcgccgcatc aaaaccagga
    44701 acttccgttt gttttaatac acgacgctca ccggtatcca tatccagttc aaacaaagtg
    44761 tctggtgtgg tcatggaaga ataaccataa cgcaatcgcg cggtttcagg ttctggattg
    44821 taggcaatcc aggtcacata ggccggatca tcaaaggcaa tgccaatgac ttcccgggtc
    44881 ttgcggttaa tttggcgcaa actggttaac ccgcgctgac gctcttcaac caccagccag
    44941 tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc
    45001 cactgttgct catcacgcat acgggtacgg tataagccaa agtttttgcc gtggcgattg
    45061 gaacgcagat aaaaacgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc
    45121 ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg
    45181 gtggtggcgc tggccaaatg aatgactaca tagtgcttcg aggtcgtttt atgcaggctg
    45241 acgtaatagg tatcgtcttt ttcttcgtag atcagtttat cttgcgatgc tggcgtaccg
    45301 atggcgtgac gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag
    45361 aaagtccagg agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg
    45421 taccagttac cagtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa
    45481 tcttctgcca gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa
    45541 aactcactat gagctgcgcg cttattggca tcgagcaatg tttcccactc atcccactct
    45601 tcactgaatg ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc
    45661 cgatagcggt agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg
    45721 atgatttcct ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg
    45781 taactatttt cttgttgcag gtagtccagg acttctggct gagaacgcgt atcgtcccgc
    45841 agccagtagt aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg
    45901 gcggcttttg gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa
    45961 actcaccggg gatgcaagcg aaacagggga gtcattgctt agatgatgac aggtaatggc
    46021 gcggatatcg aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcgattc
    46081 tggatagccc gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt
    46141 gcgtttttgc tgttcgagat cgcgttcaat gccgtcacga acatcctggg ggattttcag
    46201 cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc
    46261 gcaactcagg aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg
    46321 ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac
    46381 gccggcttca cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc
    46441 acttttagcg gcaaagacca gcgcaaggat caaacgttct gtacgctcat ccagcggcgt
    46501 actttgcgcg ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt
    46561 attccacagc accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata
    46621 tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa
    46681 tgcgcctggc actaacagtt tgcccaatcc ctgatccgca gacgaggagg tagaagaact
    46741 gctttgccca agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata
    46801 accattttta gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa
    46861 aagatgcgca aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg
    46921 tatttactgt atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt
    46981 ggcattgttt gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt
    47041 gtggtgcgtt ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt
    47101 atcagaagcc attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc
    47161 tggtaatgaa tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct
    47221 ttaccctgaa tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt
    47281 tgatcatctg cccagcgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca
    47341 attccggcag catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta
    47401 ttggcagcga aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt
    47461 tttttcatgt ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag
    47521 gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg
    47581 acctgtgggg acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct
    47641 gccagccata gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc
    47701 agccaggtgt caagcagatc cgccgtatca agcattttat cataagcatc ggcttccttt
    47761 ttgctggtaa acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc
    47821 gccatttgca gcctctcata ataactgtga ttttatacag tatatttctt ttcggttgag
    47881 aaatcaacat cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt
    47941 aatcagggga tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc
    48001 gtgatgttat taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc
    48061 ggcgtagagg tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat
    48121 cgctcccatg tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa
    48181 aaaccacatt atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt
    48241 gaagaggaag gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc
    48301 gagggtatcc gtcgcctggc ggcagaacag ctgcagctgc ccacttccac ttatcgtttt
    48361 gccgatagcg aaagcctttt ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta
    48421 aaaccggtga tgagctcttc cggcaagggg cagacgttta ttcgttctgc agagcaactt
    48481 gctcaggcat gggaatacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt
    48541 gaaggcgtcg ttaagtttga cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc
    48601 gtccatttct gtgcaccagt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg
    48661 caaccacagc aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg
    48721 gtgctggcac tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag
    48781 gtgattttca gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct
    48841 caagatctct cagagtttgc cctgcatgta cgtgccttcc tcggacttcc tgttggcggg
    48901 atccgtcagt atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat
    48961 gtcacgtttg ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt
    49021 aagccggaaa ttgatggcag ccgtcgtctg ggtgttgcgc tggcgaccgc tgagagtgtt
    49081 gttgacgcca ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc
    49141 cggcaaaaaa acgctacaaa aatgcccgat cgtagatcgg gcgttttgac ttttacagct
    49201 tagcgccttc tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca
    49261 gcgcatctgc cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt
    49321 agtcacggta gttagccgga gaaataccac ccgtcgggca gaaacggacc tgggagaacg
    49381 gacccgcgat cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga
    49441 actctttcaa accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa
    49501 ttagaggaat agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa
    49561 ttgcgaactg tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac
    49621 cggcacccac aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac
    49681 actcggtacg cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca
    49741 tcggcactgc gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca
    49801 ggattgattc tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa
    49861 atttgtcgtc ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac
    49921 gcaaggcgct gaataattca cgtcctgtcc ccacgcgtga cgcgctcagg tcaggaatgt
    49981 gcggttcgcg agcagccagt tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc
    50041 cattcacacg aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt
    50101 ctggtgttac gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg
    50161 taactaacgc aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat
    50221 gtaattctgg cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc
    50281 ggtccagcaa acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg
    50341 ctggcgcttc aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc
    50401 ccaggttacc gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag
    50461 cgatcacatt gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat
    50521 tcagccatgg ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt
    50581 catgcagcag gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct
    50641 ggaagtggtt aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat
    50701 cagaaaggtc agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca
    50761 ccaggtgcat ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca
    50821 ccaccacttt ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca
    50881 tgcgtgtaac ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat
    50941 gaacaaaaga agagcctggc aactgcatcc ccataaattc caccaccatc tggttggtgt
    51001 tggcagtacc gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca
    51061 gtaaggccat gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt
    51121 tatttggcaa accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa
    51181 acgacagggc tgccatcgtc agacccggga caatcttgtc gcacacaccg aggaacagag
    51241 caccatcaaa catgttatgg gacagcccga ccgccgcaga catcgctatc acttcgcggc
    51301 ttagcagcga caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa
    51361 caccgcccgc aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg
    51421 gatagtgttc ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga
    51481 tattgttacg caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac
    51541 cgtgtgccag gttaccgcat gccaactgcg aacgatgaac ggtcgaagtt ttcgcttgtt
    51601 ctatccgggc gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta
    51661 cgcgtaacaa ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt
    51721 cacaacgcgt ttcattcaga ggatttatga ctgaaacgcc tgtaaccgga gctcataggg
    51781 caaaaacgtt tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg
    51841 aaagtgtaaa aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa
    51901 aggataagcg cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg
    51961 ccaccgaggc aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca
    52021 tcgcccacgc ctcagtaatg gagtctaccc atttccaggc ttcttccact tcatcacgac
    52081 gtacaaacag tgcctgaata ccacgcatgg tttccagcag cagacgttca taggcatccg
    52141 ccagatgcgt ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt
    52201 tatgtttgtg gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag
    52261 gttgcagacg gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat
    52321 tcagttcagg tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac
    52381 cagtacgcag gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg
    52441 ccacgaaggt ttctgtattg ctgctcttgt tcgcgccctc ttcttccaga tatcccggca
    52501 cttttttgcc ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt
    52561 tggagcggtc gatgcggcgc agagacttca gtactttcac tttttcatcg cggatgctgt
    52621 ctgcgctcag gtcagacggc ggagacatcg caatcatgca gagaatttgc agcaggtggt
    52681 tttggatcat atcgcgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc
    52741 ccacttcttc tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca
    52801 caaacaggga gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat
    52861 agtggtcgat acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt
    52921 cctgcgaggt cgccagcgac gtccccagcg gtttctccat aactacgcgt gccggtttag
    52981 cattcagttt tgcctcacca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg
    53041 caaagtagtt aatggtgata cgattttttt gatccagcat cgcgccgaga cggctgaatg
    53101 cagcagtgtc attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata
    53161 aaccttcatc aatagtttct ttcatgaaag tttcgagcgc ctcacggaca actttggtat
    53221 aagccgcttt atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct
    53281 gaccggcttt ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt
    53341 tcgcgccgaa aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct
    53401 ccttaagtta actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca
    53461 ctgtactgct tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg
    53521 gaaaagcgcg caaagtgcgg caaaactgat aaaaaaatca tcgttttcca ttcggtaaaa
    53581 atctgacact gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta
    53641 acacggcaca caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa
    53701 tcgtcattac ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc
    53761 tggaacattt gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata
    53821 acgcgatcca ttcgagtatt gctgctctgg cgctggaagc caatgttagc gaaccgacgg
    53881 tgaatcgttt ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg
    53941 cacaaagtct ggcgaatggc actccctatg ttaatcgcaa tgtcaatgaa gatgacagcg
    54001 ttgaatcata cacagggaaa atatttgagt ccgcaatggc aacgcttgat catgtccgtc
    54061 attcactgga taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa
    54121 tcgccttttt cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct
    54181 ttcgttttaa tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta
    54241 tgaattgtag cgacggagac gtggtggtgc tgatttctca cactggaaga acaaaaaatc
    54301 tggtcgagct ggcgcagctg gctcgcgaaa acgacgcaat ggtgattgcc ctcacctctg
    54361 cgggtacccc gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata
    54421 ctgacattta tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg
    54481 cgacaggatt tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg
    54541 aagcgctgaa ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa
    54601 aacgactgtc actgtcctaa tcttatacga catccgaatg agattaattt atcgccatcg
    54661 cggcgttatt tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag
    54721 tattacatgt ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca
    54781 gatcgcgata ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac
    54841 ttttctcacg gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc
    54901 gcaagactgg ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta
    54961 tccaccttta aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc
    55021 aacctgggta aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct
    55081 gctgacgtcg tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta
    55141 ctggaagttc agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac
    55201 aataaaggta tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac
    55261 aaagcagaca ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca
    55321 cgctgtggcg aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg
    55381 aaaattgttg ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac
    55441 atcatcctcg cctctgacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc
    55501 gacccggaac tggtcggcat tcagaaagcg ttaatccgtc gtgcgcgtca gctaaaccga
    55561 gcggtaatca cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt
    55621 gcagaagtca tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct
    55681 gcagaaactg ccgctgggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc
    55741 ctgggtgcgg aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc
    55801 gacaatgtgg aagaagctat tgccatgtca gcaatgtacg cagctaacca cctgaaaggc
    55861 gttacggcga tcattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc
    55921 agctctggtc taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct
    55981 ctctatcgtg gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc
    56041 agcgaagcgg ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt
    56101 gtcacccagg gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg
    56161 gtagagtaag tacgttgccg gatgcggcga aaacgccaca tccggcctac agttcaatga
    56221 tagttcaaca gatttcgaat attctgaagc aaacttgaac ttatcatcag gcgaaggcct
    56281 ctcctcgcga gaggcttttt ttatttgatg ggataaagat ctttgcgctt atacggctgg
    56341 atttcgcccg gtttgcgagt tttcagcaat tttagtatcc aggtgtattg ttctggtcgc
    56401 ggaccaacaa aaatctcgac ttcttcattc atccgccgcg caatcgtatg atcatccgcc
    56461 tctaacagat catccatcgg tgggcgcacc tgaatcgtca gacggtgcgt cttgccatca
    56521 taaatcggaa acagcggaac aacgcgcgca cggcacactt tcatcaaacg accaatcgcg
    56581 ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt cgctgtgctc tgggccatga
    56641 tcctgatcgg gtaaataata tccccagtac ccctgacgta ccgactggat gaatggttta
    56701 ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac gacgcaccgt gttccagaca
    56761 taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg ccattttctg cccttgcgag
    56821 gccatcagca tggcaggaat atcgacggcc caaccgtgcg gcaccagaaa gataactttc
    56881 tcgttattac gcctcatctc ttcgatgatc tccagccctt gccagtcaac gcgcggctga
    56941 attttctccg gtccgcgtat tgccaactca gccatcattg ccatcgcttg cggcgcggtg
    57001 gcaaacatct catcaacaat cgcttcgcgt tcagcttcac tacgttctgg aaagcagagc
    57061 gacaaattga ttaacgcacg acggcgtgag ctttttccca gtcgtccggc aaaacgtccc
    57121 agccgtgcca gaatgggatc acggaacttt ggcggcgtta aagcgatacc cgccatcgct
    57181 gctacaccca gccatgctcc ccagtagcgc gggtggcgaa aggatttatc aaactcagga
    57241 atgtattcgc tattattttt tttcgtttcc atgcttttcc agtttcggat aaggcaaaaa
    57301 tcaatctggt gatagtgtag cggcgcaact tgccccgcac caaataaaaa aagccggtac
    57361 tgacttcgta ccggctgcga atggatgtta attaatcaaa ccgtagctgc ggcacaatct
    57421 ctttggcctg cgccaggaat tcgcgacgat cggagccggt cagcccttcg gtacgcggca
    57481 gttttgccgt cagcgggttt acggcctgct ggtttatcca tacttcatag tgcaaatgcg
    57541 gcccggttga acgtccggta ttaccggaaa gcgcgatacg gtcgccacgt ttcaccttct
    57601 gtcccggttt caccagaatc ttgcgcaagt gcatataacg cgtggtgtag ctgcgaccat
    57661 gacgaatagc cacataataa cctgctgcgc cactgcgttt ggcaaccacc acttcaccgt
    57721 cacccactga aagcactggc gtaccctgcg gcatggcgaa atcaacacct ctgtgtggtg
    57781 caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa gttagacgag atacggaact
    57841 gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc agtaccgtta cgatcgtaga
    57901 atttgccatc ttcagcgcgg atagcgtaat aatctttacc ttctgaacgc aaacgtacgc
    57961 ccagcagctg gctttgctca cgtttaccat caagcatttc tcgagacatt aataccgcaa
    58021 attcatcgcc ttttttcagt ttgcggaaat ccatttgcca ctgcatggct ttaatcactg
    58081 cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct ggcaacaaag ctccccccga
    58141 cggtaccttt cagcagattg ttaacccact ctccttgctg catttcgctg gtcattttaa
    58201 aaccgttagc ggcagtacgg tcataggttc gggtttcacg acgagacact tcccaggtga
    58261 ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag ttgttgaccg attttcaggt
    58321 tacgcaattc tttgtcggca gcagccagtt gggtgatatc acccatatca ataccatact
    58381 gattgagaat gctgcttagc gtatcgccag tggaaacaac atattcatgc acgcccgctt
    58441 caccggcgat tttgtcatcc agttcgtcct ggggaatggc ttcatcttct tgtgcagctt
    58501 gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat ttcgttctgt tccagctcaa
    58561 tggttttgac aattggcgtg gcatcgcggt gataaacata gggccgccag acagcgacgg
    58621 ccagagtaag aacggtgagc gaccccaaca taacgcggtg tggtcgcggt aaattattaa
    58681 acgccagggc gacagagcgg gctatctgtt gcacgtaatc acttcctcat taatctcctt
    58741 tcaggcagct cgcatactgg ttggctaatt gactcaggaa ttctgaatag cttgttttac
    58801 ccagtttgat attcgtcccc aggggatcca acgttcccat acgaacggat gtccctcgtg
    58861 cgacgctctc aacgaccgct ggcctgaact gtggctcagc aaaaacgcag gttgcttttt
    58921 gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc gccaggttga atctcagggt
    58981 taacggtaaa atgaccaagc ggtgtcagtc caaactgttt ttcgaaatag ccgtaagcat
    59041 cgtgaaaaac gaaataacct ttccccttga gcggcgcgag ctcgttacca acctgcgttt
    59101 cggttgaggc taattgtgcc tcaaaatcct tcaggttggc gtcaagtttg gctcgacttt
    59161 gcggcataag ttccactaat tttccatgga ttgcaaccgc tgtagcccgc gctatctctg
    59221 gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg atcttcgtca cttttttccg
    59281 cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat cagcagcggt ttcacatctt
    59341 ctagctgtgc aatcgttacc tgtttcgctt ctggtaattt acttaccggt ttttgcataa
    59401 acgcttccat ctccgggcca acccaaacga ctaagtccgc gttctgtaag cgttttacat
    59461 ccgatggacg cagtgaataa tcatgttctg aagcgccgtc aggcagtaaa acctccgttt
    59521 ctgttacacc atcagcaatg gcagaagcga tgaacccaac gggtttaagc gaagcgacaa
    59581 cggcggcatc tgcggcctgt gttgcacctc cccagagagc ggcggataat gctgcgaaaa
    59641 gaagcgtttt tttatgtaac ataatgcgac caatcatcgt aatgaatatg agaagtgtga
    59701 tattataaca tttcatgact actgcaagac taaaattaac atgacaagtc tggtttccct
    59761 ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc tctgatgtgt cgctggaact
    59821 taaacctgga aaaattttga ctttacttgg gccaaatggc gcaggtaagt cgacactggt
    59881 acgggtagtg ctcgggctgg taacacccga tgaaggggtt atcaagcgta atggaaaact
    59941 gcgcatcggc tatgtaccgc agaagctgta tctcgacacc acgttgccac tgaccgtaaa
    60001 ccgtttttta cgcttacgcc ctggtacaca taaagaagat attttgcctg cactgaaacg
    60061 tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag ctctctggtg gcgaaacgca
    60121 gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa ttattagtgc tggatgaacc
    60181 cactcaaggc gtggatgtaa atggccaggt ggcgttatat gaccttattg accagttgcg
    60241 tcgcgaactg gattgtggcg ttttaatggt ctctcacgat ctgcatctgg taatggcaaa
    60301 aaccgatgaa gtgctgtgtc tgaatcacca catttgttgt tccggcacac cggaagttgt
    60361 ttccctgcat ccggagttta tttcaatgtt tggtcctcgt ggtgctgaac aactgggtat
    60421 ctatcgccat catcataatc atcgtcacga tttacaggga cgaattgttt tgcgtcgggg
    60481 aaatgatcgc tcatgattga attattattt cccggttggt tagccgggat catgctcgcc
    60541 tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc gtatgtctta tttcggtgat
    60601 acgctggctc atgcctcatt acttggtgtc gcgtttggtt tgttgctgga cgtgaatcca
    60661 ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg gtctggtatg gctggagaag
    60721 cgtccacagc tggcgatcga cacgttatta gggattatgg cgcacagtgc cctgtcgctg
    60781 ggcctggtgg tcgttagtct gatgtctaat attcgtgttg atttgatggc ttacctgttc
    60841 ggtgatttgc tggcagtgac gccagaagat ctcatctcta ttgcgattgg cgtggtcatc
    60901 gtggtggcta ttttgttctg gcaatggcgc aatttgctgt cgataacgat tagcccggat
    60961 ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat tgttgttgat gctggtgacg
    61021 gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt tgattattac ttcgttgctg
    61081 attattcctg cagctactgc gcgtcgcttt gcccgtacgc cggaacagat ggctggtgtc
    61141 gctgttttgg tggggatggt ggcagtaact ggcggtttaa ccttttccgc gttttacgat
    61201 acgccggcgg gtccgtcggt ggtgctatgt gcggcactgt tatttattct cagtatgatg
    61261 aaaaagcagg ccagctaatc tatcgctgaa cacatttgtc ggatgcggcg cgagcgcctt
    61321 atccgaccta cggttcgcta tctctggtag gcctggtaag acgcgaatag cgtcgcatca
    61381 ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc cgacttacgg catttctggc
    61441 ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca tacgcccacg cggtgtacgc
    61501 tgcaaaaagc cttgctgaat caaataaggt tccagcacat cctcaatggt ttcacgttct
    61561 tcgccaatgg ctgccgcaag gttatccaga cctaccggcc caccaaagaa cttatcgatt
    61621 accgccagca ataacttgcg gtccatataa tcgaaacctt cagcatcgac attcaacata
    61681 tccaacgcct gagcagcgat atctgccgag atggtgccat cgtgcttcac ttcggcgaaa
    61741 tcacgcactc gacgcagcag acggttggca atgcgcggcg taccgcgagc tcgacgagca
    61801 acttccagcg cgccgtcatc actcatctca agccccataa agcgtgcgct gcgactgacg
    61861 atatattgca gatccggcac ctgataaaac tccagacgct gcacaatacc aaaacgatcg
    61921 cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac caatcagggt aaacggcggc
    61981 aaatcaattt taatggagcg tgccgccgga ccttcaccaa tcatgatatc cagttggtag
    62041 tcttccattg ccggatacaa cacctcttcc accactggcg aaagacggtg gatctcatca
    62101 ataaacaaca cgtcatgcgg ttcaaggtta gtgagcatcg ctgccagatc gcccgccttt
    62161 tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca tttcattggc gacgatgttg
    62221 gcaagcgtag ttttacccaa ccccggagga ccgaaaatca acagatgatc gagggcatcg
    62281 ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg aacgaacctg cggctgacca
    62341 acatactctt ccagtaattt agggcgaatg gcgcgatctg ccacatcttc cggcaaagtg
    62401 gtaccggcag aaatcagacg gtctgcttca atcatccttt acctcataac gcggcgcgta
    62461 gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg agcgattttg ctcaccatgc
    62521 ggcttgcctc ttgtggttta tagcccagcg ccaccagcgc ggcaactgct tcttgttcag
    62581 catcgtcggt cgccgggctg gcaggtgatg tgagtaccag gtcggcggct ggcgtaaaga
    62641 gatcgccatg caaaccttta aatcggtctt tcatttcaac aatcaagcgt tcggcggttt
    62701 ttttgccaat acccggcagt ttcaccagcg cccccacttc ttcacgctca acggcattaa
    62761 cgaactgctg cgctgacatt ccggagagga tcgccagcgc caacttcggg ccgacgccgt
    62821 tggttttgat caactctttg aacaatgtgc gctcttgttt attgttaaaa ccgtacagca
    62881 gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat cgcttcctga cccgcttcag
    62941 ggagttcata aaaacaggtc atcggcatat gcacttcata gcctacgccg cccacttcaa
    63001 ttaacaccag cgggggttgt ttttcaatga tgatgcctct gagtctgcct atcacatgac
    63061 gctcctgcgt aatgaatcaa agataatgct gtatgataaa aaaatgctgg atagatatcc
    63121 agcgaaggat gaagaaaact tgcgaggtgt cccgatgatc tgaaaaatgg
//
LOCUS       DAMZII010000010        61934 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZII010000010 DAMZII010000000
VERSION     DAMZII010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.010"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            298..384
                     /gene="leuL"
                     /locus_tag="POA77_000603"
     CDS             298..384
                     /gene="leuL"
                     /locus_tag="POA77_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDI6364088.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGSQH"
     gene            477..2048
                     /gene="leuA"
                     /locus_tag="POA77_000604"
     CDS             477..2048
                     /gene="leuA"
                     /locus_tag="POA77_000604"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6364089.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2048..3139
                     /gene="leuB"
                     /locus_tag="POA77_000605"
     CDS             2048..3139
                     /gene="leuB"
                     /locus_tag="POA77_000605"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI6364090.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3142..4542
                     /gene="leuC"
                     /locus_tag="POA77_000606"
     CDS             3142..4542
                     /gene="leuC"
                     /locus_tag="POA77_000606"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI6364091.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4553..5158
                     /gene="leuD"
                     /locus_tag="POA77_000607"
     CDS             4553..5158
                     /gene="leuD"
                     /locus_tag="POA77_000607"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI6364092.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5207..6385)
                     /gene="setA"
                     /locus_tag="POA77_000608"
     CDS             complement(5207..6385)
                     /gene="setA"
                     /locus_tag="POA77_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDI6364093.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6487..6618)
                     /gene="sgrT"
                     /locus_tag="POA77_000609"
     CDS             complement(6487..6618)
                     /gene="sgrT"
                     /locus_tag="POA77_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDI6364094.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6707..8362
                     /gene="sgrR"
                     /locus_tag="POA77_000610"
     CDS             6707..8362
                     /gene="sgrR"
                     /locus_tag="POA77_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDI6364095.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8396..8495
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8526..9509
                     /gene="thiB"
                     /locus_tag="POA77_000611"
     CDS             8526..9509
                     /gene="thiB"
                     /locus_tag="POA77_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDI6364096.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9485..11095
                     /gene="thiP"
                     /locus_tag="POA77_000612"
     CDS             9485..11095
                     /gene="thiP"
                     /locus_tag="POA77_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDI6364097.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11079..11777
                     /gene="thiQ"
                     /locus_tag="POA77_000613"
     CDS             11079..11777
                     /gene="thiQ"
                     /locus_tag="POA77_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDI6364098.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11891..12655)
                     /locus_tag="POA77_000614"
     CDS             complement(11891..12655)
                     /locus_tag="POA77_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6364099.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12741..13619)
                     /gene="araC"
                     /locus_tag="POA77_000615"
     CDS             complement(12741..13619)
                     /gene="araC"
                     /locus_tag="POA77_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDI6364100.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13958..15658
                     /gene="araB"
                     /locus_tag="POA77_000616"
     CDS             13958..15658
                     /gene="araB"
                     /locus_tag="POA77_000616"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI6364101.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15669..17171
                     /gene="araA"
                     /locus_tag="POA77_000617"
     CDS             15669..17171
                     /gene="araA"
                     /locus_tag="POA77_000617"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDI6364102.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17340..18035
                     /gene="araD"
                     /locus_tag="POA77_000618"
     CDS             17340..18035
                     /gene="araD"
                     /locus_tag="POA77_000618"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI6364103.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18110..20461
                     /gene="polB"
                     /locus_tag="POA77_000619"
     CDS             18110..20461
                     /gene="polB"
                     /locus_tag="POA77_000619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDI6364104.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20626..23532
                     /gene="rapA"
                     /locus_tag="POA77_000620"
     CDS             20626..23532
                     /gene="rapA"
                     /locus_tag="POA77_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDI6364105.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            23544..24203
                     /gene="rluA"
                     /locus_tag="POA77_000621"
     CDS             23544..24203
                     /gene="rluA"
                     /locus_tag="POA77_000621"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDI6364106.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24320..25135)
                     /gene="djlA"
                     /locus_tag="POA77_000622"
     CDS             complement(24320..25135)
                     /gene="djlA"
                     /locus_tag="POA77_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDI6364107.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25390..27744
                     /gene="lptD"
                     /locus_tag="POA77_000623"
     CDS             25390..27744
                     /gene="lptD"
                     /locus_tag="POA77_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDI6364108.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27797..29083
                     /gene="surA"
                     /locus_tag="POA77_000624"
     CDS             27797..29083
                     /gene="surA"
                     /locus_tag="POA77_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDI6364109.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29083..30072
                     /gene="pdxA"
                     /locus_tag="POA77_000625"
     CDS             29083..30072
                     /gene="pdxA"
                     /locus_tag="POA77_000625"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDI6364110.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30069..30890
                     /gene="rsmA"
                     /locus_tag="POA77_000626"
     CDS             30069..30890
                     /gene="rsmA"
                     /locus_tag="POA77_000626"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI6364111.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30893..31270
                     /gene="apaG"
                     /locus_tag="POA77_000627"
     CDS             30893..31270
                     /gene="apaG"
                     /locus_tag="POA77_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDI6364112.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31277..32119
                     /gene="apaH"
                     /locus_tag="POA77_000628"
     CDS             31277..32119
                     /gene="apaH"
                     /locus_tag="POA77_000628"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDI6364113.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32197..32676)
                     /gene="folA"
                     /locus_tag="POA77_000629"
     CDS             complement(32197..32676)
                     /gene="folA"
                     /locus_tag="POA77_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDI6364114.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32868..34730)
                     /gene="kefC"
                     /locus_tag="POA77_000630"
     CDS             complement(32868..34730)
                     /gene="kefC"
                     /locus_tag="POA77_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDI6364115.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34723..35253)
                     /gene="kefF"
                     /locus_tag="POA77_000631"
     CDS             complement(34723..35253)
                     /gene="kefF"
                     /locus_tag="POA77_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDI6364116.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35361..36692)
                     /locus_tag="POA77_000632"
     CDS             complement(35361..36692)
                     /locus_tag="POA77_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364117.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36751..37038)
                     /gene="fixX"
                     /locus_tag="POA77_000633"
     CDS             complement(36751..37038)
                     /gene="fixX"
                     /locus_tag="POA77_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDI6364118.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37035..38321)
                     /locus_tag="POA77_000634"
     CDS             complement(37035..38321)
                     /locus_tag="POA77_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6364119.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38372..39313)
                     /locus_tag="POA77_000635"
     CDS             complement(38372..39313)
                     /locus_tag="POA77_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI6364120.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39328..40098)
                     /locus_tag="POA77_000636"
     CDS             complement(39328..40098)
                     /locus_tag="POA77_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDI6364121.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40572..42086
                     /gene="caiT"
                     /locus_tag="POA77_000637"
     CDS             40572..42086
                     /gene="caiT"
                     /locus_tag="POA77_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDI6364122.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42117..43259
                     /gene="caiA"
                     /locus_tag="POA77_000638"
     CDS             42117..43259
                     /gene="caiA"
                     /locus_tag="POA77_000638"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDI6364123.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFTECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43388..44605
                     /gene="caiB"
                     /locus_tag="POA77_000639"
     CDS             43388..44605
                     /gene="caiB"
                     /locus_tag="POA77_000639"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDI6364124.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44679..46232
                     /gene="caiC"
                     /locus_tag="POA77_000640"
     CDS             44679..46232
                     /gene="caiC"
                     /locus_tag="POA77_000640"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDI6364125.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVVNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46341..47126
                     /gene="caiD"
                     /locus_tag="POA77_000641"
     CDS             46341..47126
                     /gene="caiD"
                     /locus_tag="POA77_000641"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDI6364126.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47132..47722
                     /gene="caiE"
                     /locus_tag="POA77_000642"
     CDS             47132..47722
                     /gene="caiE"
                     /locus_tag="POA77_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDI6364127.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47808..48203)
                     /gene="caiF"
                     /locus_tag="POA77_000643"
     CDS             complement(47808..48203)
                     /gene="caiF"
                     /locus_tag="POA77_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDI6364128.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48464..51685)
                     /gene="carB"
                     /locus_tag="POA77_000644"
     CDS             complement(48464..51685)
                     /gene="carB"
                     /locus_tag="POA77_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI6364129.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51703..52851)
                     /gene="carA"
                     /locus_tag="POA77_000645"
     CDS             complement(51703..52851)
                     /gene="carA"
                     /locus_tag="POA77_000645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI6364130.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(53307..54128)
                     /gene="dapB"
                     /locus_tag="POA77_000646"
     CDS             complement(53307..54128)
                     /gene="dapB"
                     /locus_tag="POA77_000646"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI6364131.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(54295..55209)
                     /gene="rihC"
                     /locus_tag="POA77_000647"
     CDS             complement(54295..55209)
                     /gene="rihC"
                     /locus_tag="POA77_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDI6364132.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(55275..56225)
                     /gene="ispH"
                     /locus_tag="POA77_000648"
                     /gene_synonym="lytB"
     CDS             complement(55275..56225)
                     /gene="ispH"
                     /locus_tag="POA77_000648"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDI6364133.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56227..56676)
                     /gene="fkpB"
                     /locus_tag="POA77_000649"
     CDS             complement(56227..56676)
                     /gene="fkpB"
                     /locus_tag="POA77_000649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6364134.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56764..57258)
                     /gene="lspA"
                     /locus_tag="POA77_000650"
     CDS             complement(56764..57258)
                     /gene="lspA"
                     /locus_tag="POA77_000650"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI6364135.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57258..60074)
                     /gene="ileS"
                     /locus_tag="POA77_000651"
     CDS             complement(57258..60074)
                     /gene="ileS"
                     /locus_tag="POA77_000651"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI6364136.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60117..61058)
                     /gene="ribF"
                     /locus_tag="POA77_000652"
     CDS             complement(60117..61058)
                     /gene="ribF"
                     /locus_tag="POA77_000652"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDI6364137.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61066..61284)
                     /locus_tag="POA77_000653"
     CDS             complement(61066..61284)
                     /locus_tag="POA77_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDI6364138.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61387..61650
                     /gene="rpsT"
                     /locus_tag="POA77_000654"
     CDS             61387..61650
                     /gene="rpsT"
                     /locus_tag="POA77_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI6364139.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14850 a        16913 c        16157 g        14014 t
ORIGIN      
        1 tcgcaaaaca tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg
       61 tccccttttt cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa
      121 aagcgtcggt agttaagcag aaattaatat cgcttacttt aaccaccgca gcacaattag
      181 ctaattttac ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca
      241 tccgtttttg tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg
      301 actcacatcg ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga
      361 cgagtgagcg gcagccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg
      421 cgcgggtttt tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga
      481 gccagcaagt cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa
      541 gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg
      601 tgatggaagt cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg
      661 cccgccaggt taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggag aaagatatcg
      721 acgtggcggc cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcca
      781 cttcgccaat gcacatcgcc accaagctgc gcagcacgct ggatgaagtg atcgaacgcg
      841 ctatctatat ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag
      901 atgccgggcg tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg
      961 gtgccaccac catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg
     1021 gaatcatcag cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtgc
     1081 atacccacga cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg
     1141 cacgccaggt ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg
     1201 aagaagtcat catggcgatt aaagttcgta aggatattct caacgtccac accgccatta
     1261 atcaccagga gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc
     1321 cggcaaacaa agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg
     1381 atggcgtgct gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga
     1441 accaaatcca gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg
     1501 atgagatggg gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga
     1561 agctggcgga caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg
     1621 gtaagcagca agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct
     1681 ctaacgatat cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag
     1741 ccgccaacgg taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact
     1801 ataacgtcga actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg
     1861 gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca
     1921 ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg
     1981 ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa
     2041 ccgtgtgatg tcgaagaatt accatattgc cgtattgccg ggggacggta ttggtccgga
     2101 agtgatgacc caggcgctga aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat
     2161 caccaccagc cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc
     2221 gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg
     2281 tccgaaatgg gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgttgccttt
     2341 gcgtaagcac ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga
     2401 agcattctgt ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg
     2461 cgaactgacc ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata
     2521 tgaaaaagcg tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat
     2581 cgcgtttgaa tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt
     2641 gctgcaatcc tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga
     2701 tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc
     2761 acagtttgac gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc
     2821 aatgatcact ggctcgatgg ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg
     2881 tctgtatgaa ccggcaggcg gctcagcacc agatatcgca ggcaaaaaca tcgccaaccc
     2941 gattgcgcaa attctgtcgc tggcactgct gctgcgctac agcctggatg ccgatgatgc
     3001 ggcttgcgcc attgaacgcg ccattaaccg cgcattagaa gaaggcattc gcaccgggga
     3061 tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta
     3121 tgtggcagaa ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac
     3181 gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat
     3241 gaagtgacct caccgcaggc gtttgatggt ctgcgcgccc acggtcgccc ggtacgtcag
     3301 ccgggcaaaa ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat
     3361 gcctgtggtg aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt
     3421 ggcgtcgagc tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg
     3481 gaacagggcg tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc
     3541 cacggcgcgt ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg
     3601 gcaacacaaa ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa
     3661 gccgcgccgg gcattacagc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc
     3721 gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg
     3781 gaaggtcgta tgaccctgtg caatatggca atcgaaatgg gcgcaaaagc cggtctggtt
     3841 gcaccggacg aaaccacctt taactatgtc aaaggccgtt tgcatgcgcc gaaaggcaaa
     3901 gatttcgacg acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc
     3961 gataccgttg tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat
     4021 ccaggccagg tgatttccgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg
     4081 gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggcattccg
     4141 ctgaccgaag tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa
     4201 gatttacgcg cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca
     4261 ctggtggttc ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa
     4321 atctttattg aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg
     4381 aacaacgacc gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa
     4441 ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct
     4501 gctgtgaccg gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga
     4561 gaaatttatc aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga
     4621 tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct
     4681 gtttaacgac tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct
     4741 gaacttcccg cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg
     4801 ctcctcgcgt gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc
     4861 gccgagtttt gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa
     4921 attaagcgat gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca
     4981 tttcgacgtg gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac
     5041 catcgatgcc ttccgccgcc actgcatgat gaacggtctg gacagtattg ggcttacctt
     5101 gcagcacgac gacgccattg ccgcttatga agcaaaacaa cctgcgttta tgaattaatc
     5161 cccttgcccg gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa
     5221 cctttgcggt taaaaataat gcgacaacag aaataaccgc aattacccag tagacagcaa
     5281 agtgccccca actttgtgca attgctccct gaataacgcc agccagaatt accccggtag
     5341 aaatactgtt agtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa
     5401 accatagcat cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg
     5461 tcatcaacgc catacggcta tgaaagaaaa tcaatccggt gtaaaacagt actcctgccg
     5521 ccactgctat gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa
     5581 tcattgctgg tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg
     5641 gcaatcctaa ctcgctactg atccacaacg gcatatcaat aatgtacatg gtgttgcagg
     5701 tccacattaa cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagccacctt
     5761 gcattgataa agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg
     5821 caatcaatac cagactgagt gtgaatatcc cggcggcaat cgaaaacatc accgtaaagc
     5881 cgtaattcaa cgccagcata aaggccaacg gtggaccgat aacccatgcc agagaaagct
     5941 gcgcacgcat caccgagcta aacatcacca cttctcgcgc cgagttatcc gcatattccc
     6001 gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac
     6061 aggtgataag cgtcagataa tgacgattaa atgcaaacaa tagcgcattg ccgatagcca
     6121 tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca
     6181 accagaggct tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga
     6241 aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc
     6301 cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc
     6361 gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa
     6421 ccagcaggta taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca
     6481 ggcaagtcaa ctttcagaat tgcggtcatc ccactgcatc agttcttcaa gcatggttaa
     6541 tcgttgtggg acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg
     6601 ctgataaaac tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat
     6661 atagggaaaa taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg
     6721 tctgcaacaa cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac
     6781 gctcaacgaa ctggcagcgt tattgagctg ctcgcgtcgt catatgcgca ccctgctcaa
     6841 caccatgcag gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgtg gtaaacgctc
     6901 gcgtctgaca ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct
     6961 ggagcaggat cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca
     7021 aatgctggtt tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct
     7081 ctactatcgt ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca
     7141 tattgcccgg caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga
     7201 agcagacatc gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg
     7261 tccaggagtc cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt
     7321 aaaacgaatc aatacgctgc cgctctattc gcatattgcg gacattgtgt cgccgacgcc
     7381 ctggacgctg gatatccatc tcacgcaacc ggaccgctgg ttaccgttac tgctgggaca
     7441 agttccggcg atgatcctgc cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc
     7501 catcggcacc ggtccgtatg cggtgatccg caacaccacc aatcaactga aaattcaggc
     7561 attcgatgac ttcttcggtt accgggcatt aatcgatgaa gttaacgtct gggttctgcc
     7621 ggaaattgcc gacgagccag ctggaggtct gatgctaaaa ggtccacagg gtgaggaaaa
     7681 agagattgaa agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca
     7741 tcgcggggcg aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct
     7801 ggtctatttc gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc
     7861 ccgttggcac catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac
     7921 cctgaccttt tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat
     7981 tctggcaagt caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga
     8041 aggagagatc gagagcgata tctggcttaa cagcgccaac tttacgctgc cgctggattt
     8101 ttcgctgttc gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg
     8161 gcaagccgac gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca
     8221 actggtcgcc agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca
     8281 acgcagtatg cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg
     8341 gtttgcgccg ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt
     8401 ctcaacgggg tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga
     8461 taacgccggc gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt
     8521 gcaaagtgtt aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta
     8581 aacccgttct gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa
     8641 agatcaaaaa agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag
     8701 atggcgtttc gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg
     8761 tgctggggct ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa
     8821 gcggtgtggc agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc
     8881 cgtttgatta cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc
     8941 aaagcctgaa agaattggtt gagagcgatc aaaactggcg ggtgatttat caggatccgc
     9001 gcaccagtac gccggggctg ggcctgctgt tgtggatgca aaaagtctat ggcgatgacg
     9061 ccccacaagc ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg
     9121 aagcctacgg cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc
     9181 cggcttatca cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag
     9241 gtcactatct gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg
     9301 cgcaaaaatt cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta
     9361 actggatgta tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac
     9421 cggccaccac gctggagttc acaccagctg aagtggctgc acaacgtcag gcatggatta
     9481 gcgaatggca acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc
     9541 cgccacgctg gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc
     9601 gcagggtaac tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc
     9661 cttctggcag gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg
     9721 cgccctctat cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac
     9781 cttgatcctc ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg
     9841 ctggctggca tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct
     9901 gcaaggcatt ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact
     9961 ccaggcactg gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg
    10021 cggctggcat tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt
    10081 tgccgcgctt atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg
    10141 cggtccgcag gcaactacta tcgagctggc tatttatcag gcgctgagtt acgactacga
    10201 tcctgcccgc gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct
    10261 gttgagtcag cgactaagta aggccattgc gcccggtacc acgctgctgc aaggctggcg
    10321 cgatccggac gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct
    10381 gctgctgttg ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc
    10441 ggaagtgctg gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct
    10501 ggcggcaggt gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact
    10561 gcgggcgcgg cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct
    10621 cgccatgccg gggattgtgc tggctaccgg cttcttttta ctgctcaaca acaccattgg
    10681 cctgccgcaa tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta
    10741 tgcgctgaaa gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg
    10801 tcagtcgctg ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa
    10861 acgtccactg gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt
    10921 agtggcgttg ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat
    10981 tggctcctat cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt
    11041 tctgctgttt accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca
    11101 cctggcttta tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg
    11161 tggcaatcct cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt
    11221 ttctgacgcc agccagcggt tcgctgacta tcgacagcgt tgatcacacc acgacgccgc
    11281 cgtcacgccg cccggtatcg atgctgtttc aggagaacaa cctgttcagt cacctgacgg
    11341 tcgcgcagaa catcgggctg gggcttaatc cgggattgaa gctgaacgcg gcgcagcagg
    11401 agaaaatgca cgctatcgcc cgccagatgg ggattgataa tttaatggcg cggttaccgg
    11461 gcgagctttc cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac
    11521 agccgatttt attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga
    11581 tgctgacgct ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc
    11641 acagtgtgga agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca
    11701 tcgcctggca gggtaagacc aatgagttgt tgagcggtaa ggcgagtgct tcggcactat
    11761 tggggattac gggttagtat gcactgtcgg atgcggcgtg aacgccttat ccgacctacg
    11821 ggtagtgcgt tttgtaggcc tgataagacg cggtcagcgt cgcatcaggc aacccgcaca
    11881 agactatctc ctaaccccca accactttac gcaaaatatc gatatacacc ggcatcaacg
    11941 ggtggcgaat taacaccacc agcgccacca cgccgatggc agaaattaac ggcgtcagcc
    12001 acaacaaacg gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc
    12061 ataaccgcca gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc
    12121 atttaaactc gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc
    12181 caggcaggaa gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt
    12241 ttgccaccgg cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa
    12301 caaagcgacc gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat
    12361 cgagtaatgc tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc
    12421 agcccagcca gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccagg
    12481 cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg
    12541 taccgggtag aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg
    12601 ccatcaatga atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt
    12661 gcctgtcaaa tagacgaagc agggattctg caaaccctat gctactccgt caagccgtca
    12721 attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca
    12781 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa
    12841 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
    12901 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
    12961 cctaactgct ggcggaaaag atgtgacaga cgcgacggtg acaagcaaac atgctgtgcg
    13021 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
    13081 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
    13141 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
    13201 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
    13261 aagaaacccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaagcgcgc
    13321 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
    13381 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
    13441 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
    13501 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
    13561 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
    13621 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
    13681 cgtcactgcg tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa
    13741 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
    13801 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
    13861 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
    13921 gtttctccat acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga
    13981 ttttggcagt gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc
    14041 caccagcgta gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa
    14101 ccagttccgt catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt
    14161 gcttgcagag cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacacaac
    14221 cggctcgacg cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt
    14281 tgccgaaaac ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc
    14341 ggaagagatt acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctacattgg
    14401 cggtatttat tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacac
    14461 cgccgtggcg caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct
    14521 ttccggtacc acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataagtc
    14581 gctatggcac gaaagctggg gtggcttgcc gccagccagt ttctttgatg agctggaccc
    14641 gatcctcaat cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc
    14701 ggtgggcacc ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat
    14761 ttccggcggc gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc
    14821 actggtaaaa gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt
    14881 tggcgagcgg gcagtgaaag gtatttgcgg tcaggttgat ggcagcgtgg tacctggatt
    14941 tatcggtctg gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt
    15001 actcggctgg ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa
    15061 cgccagccag aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct
    15121 ggatcacctg ccggtggtgc tcgactggtt taacggccgc cgcacaccga acgctaacca
    15181 acgcctgaaa ggggtgatta ccgatcttaa cctcgctacc gacgctccgc tgctgttcgg
    15241 cggtttgatt gctgccaccg cctttggcgc gcgcgcaatc atggagtgct ttaccgatca
    15301 ggggatcgcc gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat
    15361 tatgcaggcc tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg
    15421 ctgtgcgctc ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc
    15481 atcagcccag caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca
    15541 ggcacaacgc tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca
    15601 acactatctt ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg
    15661 acacgataat gacgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc
    15721 tgtatggccc ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc
    15781 tgaatacgga agcgaaactg ccctgcaaac tagtgttgaa accgctgggc accacgccgg
    15841 atgaaatcac cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg
    15901 tgtggctgca cactttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca
    15961 aaccgttgct gcaattccac acccagttta acgcggcgct gccgtgggac agtatcgata
    16021 tggactttat gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg
    16081 cgcgtatgcg tcagcaacat gccgtggtta ccggtcactg gcaggataaa caagcccatg
    16141 agcgtatcgg ctcctggatg cgccaggcgg tctctaaaca ggatacccgt catctgaaag
    16201 tctgccgttt tggcgataac atgcgtgaag tagcggtcac cgatggtgat aaagttgccg
    16261 cacagatcaa gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg
    16321 tgaactccat cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca
    16381 ccatgacgcc tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc
    16441 gtattgagct ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca
    16501 cctttgaaga tttgcacggt ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc
    16561 agcagggtta cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca
    16621 tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc
    16681 acttcgagaa aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga
    16741 ttgccgtaga agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg
    16801 atcctgcccg actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg
    16861 atctcggcga tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact
    16921 ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg
    16981 cttccgaagc gtggatcctt gctggtggcg cgcaccatac cgtcttcagc catgcgctga
    17041 acctcaacga tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata
    17101 acgacacccg cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat
    17161 ttcgtcgcta agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat
    17221 ctggcctaca cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattgttttc
    17281 cccctcgccc ctttggggag agggccgggg tgaggggaaa cgacctctca ggagtcaaca
    17341 tgttagaaga tctcaaacgc caggtattag aagccaacct ggcgctgcca aaacacaacc
    17401 tggtcacgct cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga
    17461 tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga cgatatggtc gtggttagca
    17521 tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc
    17581 ggctgctcta tcaggcattc ccctccattg gcggcattgt gcatacgcac tcgcgccacg
    17641 ccaccatctg ggcgcagtcg ggtcagtcga ttccagcaat cggcaccacc cacgccgact
    17701 atttctacgg caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat
    17761 atgagtggga aaccggtaac gtcatcgtag aaacctttga aaaacagggt atcgatgcag
    17821 cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc aaaaatgccg
    17881 aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc
    17941 gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc
    18001 gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc
    18061 aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg tggcgcaggc
    18121 aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag tctccttctg
    18181 gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt
    18241 tattcccgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac aaggctttcg
    18301 cctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg
    18361 cgcccatcgc caattgatga attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta
    18421 cgaggccgat gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcaccggt
    18481 gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga aaccgcatcc
    18541 cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc gccacggtga
    18601 gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc tggggccgga
    18661 gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc gcccgctgct
    18721 gctggaaaaa ctcaacgcct ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa
    18781 cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc gtcttccgct
    18841 gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta aaaacggcgt
    18901 cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaagcgc tgaaatccgc
    18961 gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg
    19021 aaaatctatc gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga
    19081 taaacctgcg ctggcaactt ataacctgaa agattgcgag ctggtgacgc agatcttcca
    19141 caaaactgaa atcatgccat ttttactcga acgagcaacg gtgaacggcc tgccggtgga
    19201 tcggcacggc ggttcggtgg cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc
    19261 tggttatgtc gcgcctaatc tcggcgaagt gccaccgcac gccagccctg gcggctacgt
    19321 gatggattca cggccagggc tttatgattc agtgctggtg ctggactata aaagcctgta
    19381 cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggttgaag gcatggcgca
    19441 acctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcac gggagaaaca
    19501 ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg
    19561 taataagccg ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgctcgg
    19621 caccaccgcc tgccgcttct tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca
    19681 tcagatcatg cggcagacca aagcattgat tgaagcgcag ggctacgacg ttatctacgg
    19741 agataccgac tcgacttttg tctggctaaa aggtgcacat tcggaagaag aggcggcgaa
    19801 aatcggtcgt gtactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca
    19861 acggctgacc agcgcattag aactggagta tgaaactcat ttctgccgtt ttctgatgcc
    19921 aaccattcgc ggagccgata ccggcagtaa aaagcgctac gccgggctga ttcaggaggg
    19981 cgataaacag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct
    20041 ggcccagcag tttcagcagg agctatacct gcgcatcttc cgcaacgagc catatcagga
    20101 atatgtacgc gaaaccatcg acaaactgat ggcgggtgaa ctggatgcgc gactggttta
    20161 ccgtaaacgc cttcgccgtc cgctgagcga gtatcagcgt aatgtgccgc ctcatgtacg
    20221 cgccgctcgc cttgccgatg aagaaaacca aaagcgcggt cgccccttgc aatatcagaa
    20281 tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg
    20341 ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat
    20401 actccctttt attgaggata attttgctac acttatgacc gggcaacttg ggctattttg
    20461 agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag
    20521 taccatagcg ccctttccat tccaggacct gaataacacc actacctcat aagcacggta
    20581 gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt
    20641 ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg
    20701 gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc
    20761 agtgattccc ccgtgacccg cgtgatgttc aaccctggtg atactattac cagccatgac
    20821 ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt
    20881 actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa
    20941 ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc
    21001 tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc
    21061 ggcctgcgcg gtcagcgtac cagcctgatt ccgcatcagc tcaacatcgc tcatgatgtt
    21121 ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt
    21181 gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctaatt
    21241 atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg
    21301 cgctttgcgc tatttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg
    21361 tttgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag caaacagcgc
    21421 ctggaacatc tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg
    21481 gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact ggcagagcac
    21541 gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga aagccacttc
    21601 gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag
    21661 cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg
    21721 agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat cgagccgctg
    21781 ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga gctggtttcg
    21841 atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg
    21901 aaaggattcc cgaaacgcga gctgcacacc attaaactgc cgctaccgac gcagtatcag
    21961 acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc
    22021 gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc cacctggtgg
    22081 aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg ctctcagaaa
    22141 gtgctggtga tctgcgccaa agctgccact gcgctgcaac tggagcaggt actgcgcgaa
    22201 cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga acgtgaccgc
    22261 gctgccgcct ggtttgccga agaagacacc ggcgcacagg tactgctgtg ctcagaaatc
    22321 ggttctgaag gacgtaactt ccagttcgcc agccacatgg tgatgtttga cctgccattc
    22381 aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca ggcgcacgat
    22441 attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt gcgctggtat
    22501 cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat ttacgatagc
    22561 gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat
    22621 ctgatcaaaa actgccgcga gcaacatgaa gcgctgaaag cacagctgga acagggtcgt
    22681 gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc
    22741 attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt
    22801 atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat
    22861 atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac ctttgatcgt
    22921 gaagtggcac tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc
    22981 aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc actgttaaaa
    23041 aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag
    23101 gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg
    23161 gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt
    23221 aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac
    23281 gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt gattgatgca
    23341 gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga agctctgcgt
    23401 gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag
    23461 ataatggaaa gcctggatca ggcaggttgg cgtctggatg ccctgcgttt gatcgttgtg
    23521 acgcatcagt aacggagctg aaaatgggga tggaaaacta caatccaccg caggaaccct
    23581 ggttggttat cctgtatcag gatgaccaca ttatggtggt caacaagccg agcggtttgt
    23641 tgtctgtgcc gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc attcagcgtg
    23701 attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag
    23761 tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccga
    23821 aaaagcagta tgtggcccgc gtctgggggc atccatcccc cgcagaaggt ctggtggatc
    23881 tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta
    23941 aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg gcaagagtgg
    24001 tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc
    24061 atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg gcaccgcgtt
    24121 tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat agtatgacgt
    24181 ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc ggataaggcg
    24241 ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat
    24301 caggcctaca gtttaaaact tatttaaacc ctttctgctg ctttatcagc tcatatgcct
    24361 gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc aaacctttcg
    24421 ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg
    24481 tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac
    24541 gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg ccaaactgtg
    24601 caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt
    24661 cagcaataac atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg
    24721 cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc
    24781 gaaactggcg catcttttcg cgcagcgggt aattgtctga ttttcccacc cggaacgcat
    24841 tttgcgccgc agtacgggaa gcgccatgaa gattcattcg gtccatcaac tggctggcga
    24901 tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa
    24961 aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac gccattttac
    25021 ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc
    25081 cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt
    25141 ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt
    25201 tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt
    25261 caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg
    25321 acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa
    25381 aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt
    25441 tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc
    25501 tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac
    25561 cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag
    25621 ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa
    25681 ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg
    25741 atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggaa
    25801 ggtgattacc agatggtggg tcgccagggt cgcggtaaag cggacctgat gaaacaacgt
    25861 ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct
    25921 gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agttgcggag
    25981 atctggaacg cccgctttaa ggtgggtccg gtaccgatct tttatagccc ctatttgcag
    26041 ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc
    26101 accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc
    26161 accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc
    26221 tacctctccc aagcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc
    26281 tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac
    26341 tccggggtca tggatcaggt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct
    26401 agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa
    26461 aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc
    26521 caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaat
    26581 tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc agtgcacttt
    26641 gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat
    26701 ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagttgct ggcaacccat
    26761 tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc
    26821 gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg
    26881 gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg
    26941 ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca atctgactac
    27001 tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag
    27061 gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt
    27121 tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg
    27181 gagaatgacg acaaaacggg ttcactggtg tgggcaggcg atacttactg gcgtatctcc
    27241 gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc
    27301 agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccgt
    27361 tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac tgctgagcaa
    27421 tataagaatg gtatttcgca ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg
    27481 tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta
    27541 ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac
    27601 ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa
    27661 cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt
    27721 ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa
    27781 ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc
    27841 gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg
    27901 cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgctca
    27961 ggcaaggcag caacttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat
    28021 catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca
    28081 gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg
    28141 cagccgtctg gcttacgatg gactgaacta caacacctat cgtaaccaga tccgcaaaga
    28201 gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca
    28261 ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa
    28321 cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc
    28381 ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct
    28441 ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg gctggggccg
    28501 tattcaggag ttgcccggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat
    28561 tgttggcccg attcgttccg gcgttggctt ccatattctg aaagttaacg acctgcgcgg
    28621 cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc
    28681 gccgatcatg actgacgaac aggcccgtgt gaaactggaa cagattgctg ctgatatcaa
    28741 gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tctcaggatc caggctctgc
    28801 taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccgg ccttccgtga
    28861 cgccctgact cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg
    28921 gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga
    28981 tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca
    29041 ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt
    29101 gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca
    29161 cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg
    29221 gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa
    29281 ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg
    29341 gggcagttag cgattgaaaa tggacattac gtggtggaga cgctggcgcg agcgtgtgat
    29401 ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt
    29461 aacgacgcag gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg
    29521 aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat
    29581 ttaccgctgc gcgatatcgc agatgctatc acccctgcgc ttttgcacga agtgattgct
    29641 attttgcatc acgatttgcg gaccaaattt ggtattgccg aaccgcgcat tctggtctgc
    29701 gggctgaatc cacacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt
    29761 attccgctgc tcgacgagtt gcgggcgcag gggatgaaac ttaacgggcc gctgcctgcc
    29821 gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc gatgtaccac
    29881 gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg
    29941 ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt
    30001 ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt
    30061 aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa
    30121 actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg
    30181 gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg gtcggcgaac
    30241 gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc
    30301 atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg
    30361 gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca
    30421 tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact
    30481 ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg
    30541 gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg gaagttccgc
    30601 cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg
    30661 caacgatgcc tcacccggtt aaagatgttc gtgtgttgag ccgcatcacc accgaagcct
    30721 ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt
    30781 taacgggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt
    30841 gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa
    30901 ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggcacaat cttcacctga
    30961 taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt
    31021 gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca
    31081 gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac
    31141 cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg aaatgatcga
    31201 tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact
    31261 cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga
    31321 actgatcgca ttgctgcata aagtagaatt tacccctggg aaagataccc tctggctgac
    31381 gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg tgaaatcctt
    31441 aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc
    31501 cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc
    31561 cgacgagctg cttaactggc tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa
    31621 gttggtgatg gcccacgcag ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg
    31681 cgcgcgcgat gtagaagcgg tgctatcgag tgactcctat cccttctttc tcgacgctat
    31741 gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa gactgcgttt
    31801 tatcaccaac gcctttaccc gtatgcgttt ttgctttcca aacggtcaac tggatatgta
    31861 cagcaaagaa tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg
    31921 ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg
    31981 tacgccggaa ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg
    32041 cctgcgctgg gaagataagc agtattttgt ccagccgtcg aaccggcata aggatttggg
    32101 cgaagcggcg gcgtcttaaa cacagcctga tataggaagg ccggataaga cgcgaccggc
    32161 gtcgcatccg gcactagccg taaattctat acaaaattac cgccgctcca gaatctcaaa
    32221 gcaatagctg tgagagttct gcgcatcagc atcgtggaat tcgctgaata ccgattccca
    32281 gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt
    32341 cagatacagt ttttgcgctt ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac
    32401 catgatttct ggtacgtcac cacacgccgc gatggcttca tccaccgact tcacccacgt
    32461 tacgcgatcg tccgtacccg gttgactgct gaggataata tttttgcgtc ctggcaacgg
    32521 acgaccgatt gattcccagg tatggcggcc cataatcacg ggtttattta aggtgttgcg
    32581 tttaaaccag gcgagatcgg caggcaggtt ccacggcatg gcgttttcca tgccgataac
    32641 gcgatctacc gctaacgccg caatcagact gatcattgag atttcccgat aaaaaaaatt
    32701 gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa
    32761 aattttatat tctgctggcg agtccacgct ctctccctgg actcgccgca ttacaatgaa
    32821 acaaaaacaa acagttagct gtaaagtgtg atttacgtca ctctttatta ggatgagggt
    32881 ttcgtttccg gttcatccgc catgttgccg gtatgtttac cttcttcggt tccctgccag
    32941 ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg taatgatctc acttaacatc
    33001 gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt cgttctcaac cattgccatc
    33061 tcttccacca tctgaatatt aaagcggcgg aacacatcgg cacgttctcg cgcttcatac
    33121 ggccccagac ctaaactttc cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt
    33181 tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa tgtagtggtc gacatcgcgg
    33241 gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca ccatctctgt cagttgcagg
    33301 ttggtttgcg gatcgtcgat ggcgttaatc agcacttccg ctttcgccgc tccggcagat
    33361 tccagtaaat ccatccgcgt ggcatcgcca taaaacactt tcataccaaa tttacgcaag
    33421 gtttcgatat ggtccggatc gtgatcgagt accaccattt tcaccccgct ggagagcagt
    33481 aaacgtccgg taatctgccc aaaacgaccg aatccggcga taatcacgcg cggctgttct
    33541 tcgtcgatct catcggcttc acgcgcttcc tcagtagaag attgctcaag gcgattgagg
    33601 atcaccagca gaatcggcgt tgctgccatc gacagcgcca ccgccagggt cagcgatttc
    33661 gcccactccg gctccagcac attcgccatc tgcgccgcgc caaataccac aaaggcaaac
    33721 tcactgccct gccctaacaa caccgcaaac caacgacgct gtttatttgg cacttgcaac
    33781 ggtcgggcaa tcagccacag catggcgatt ttgatgatga ggaaaccgag cagcaaaatg
    33841 acaatgcgca atgggttttc aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg
    33901 aaaaacagcc ccaacagcaa acctttaaat ggttcgatat cgctctccag cgcatgacgg
    33961 tattccgagc ttgccagcag tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg
    34021 acctcttcca gcagcaaacc aaagccaaac acgaggaata acgccacggc actaaacact
    34081 tcccgcaagc cagagcgggc tacaaaacgc agcgccggac gcgtgacata gcgccccagc
    34141 aataccacca gcaccagcgc acccgccact tttaacgccg agagagcaaa tgcgcccatc
    34201 gtcgtcgagg cactgctcgt tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg
    34261 atatcctgga acagcagcac cgcaaaggca ctgcgaccca tttgcgtcac catcagattg
    34321 cgttcattca tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc
    34381 aattccgcga cctgccagcg cagcccaagt aacatgcaga acagccccag caggccaccg
    34441 caaatcacca tctgcaatgc gccgccgccg aacactgccg cacgcagctt ccatagcctt
    34501 tgtggatcga gttcgaggcc gataataaac agcatcagca ccacgccaat ctcggcaaag
    34561 tgcaggatag attcggcatc ggtcaccagt cgcagccccc acgggccaat aatgcagccg
    34621 gcaatcaggt agccaagtac cgatcccaga ccaagacgta ccgcaatggg tacaatcagc
    34681 gccgccgatc cgaggtagat cagcgcctga accagcgtat ggctatccat gatgggcctc
    34741 ctgccattcc agcagacgtt gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc
    34801 acaaataaag gtgcagtgca tggcaaacgg tggcagccag ttcagcccgc agtagattgc
    34861 cgtcgcctgt agcggctgcg acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg
    34921 gctttccccg ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc
    34981 gccgtgaccg taagcccagc cgtgcgagaa aactttatcg atccaaagtt tgaggagcgg
    35041 tggaatgctg taccactgca tcgggtgctg ccagacgatc agatcggcgc gagacagcgc
    35101 ctcctgctcg gcggcaatat cgatattgaa gtcaggatag agttgataaa gagagcgaat
    35161 ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat gggaatgatg
    35221 cggatacgga tgcgcataaa ttataagaat cataggtagc ccgcgtgatt acgcctttgt
    35281 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    35341 aagagagaaa tctgaacaat ttaccccatt ctctgcccgc ggatcgtcat attgctggtt
    35401 tgcgccagtg acatccctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    35461 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaagatc
    35521 ggcagtgccc aggtcgaaac aatggtgcca atacggctta aggacataat cacgcccacg
    35581 gcagaggcgc ggatatctgt cgggaagagt tcattaggat agagccactg caaattaccc
    35641 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    35701 tccgggatca gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    35761 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    35821 aacaggctaa tcaccacgtt ccccagtgcc gcgtttttgc caacccccaa tcccaacaaa
    35881 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    35941 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    36001 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt gatcatcatc
    36061 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc aggcagttcg
    36121 aatcgaccaa tcaaaatcaa caaacagggg atcgccgcgc tacccagcat ccagcgccag
    36181 ccgccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    36241 cacatggcgg cgataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    36301 atcattgagg tggcgatggg ataatctgca ccgatgacaa tgccgataag tacccgcatc
    36361 accaacagtt cgacggggga tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg
    36421 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggaaatata gccaaacagc
    36481 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca acaagccaat ccagtcagcg
    36541 tccagtttta gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    36601 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat tgaggagaat
    36661 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    36721 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    36781 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    36841 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    36901 acctgcgggg cacgctttca ccagcagctc cagggcctgt ttatcggcat cggccttcac
    36961 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    37021 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    37081 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    37141 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    37201 cgtgggttat caaggaaggc cggtagtttc tggtacatac gcatatcgcg cagcgggcca
    37261 ctctcaagat gctgacgata ttccgccagt ttttgcttac tgaaatcgtc gcttttcatc
    37321 gctgaaagca cggtttttgc tgcggcttcc ccggcggcaa tcgccagatc cataccgcga
    37381 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    37441 ccaaccaact ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    37501 accagcttgc cacccgcgat cagcggtgca acggccggat gttgtttgaa atcttccagc
    37561 atttgcggca ccgatttttt tgcgtcatgc agatgatgca gaccacaaac cagccccagc
    37621 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    37681 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcaattacc
    37741 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    37801 tttgccatcc ccagcttttc ggcaaggatg gagttcactc catcagcaag gatcaccgtt
    37861 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    37921 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    37981 agccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    38041 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    38101 tgggtgatca ggcgttctac cggggcggat tcggcgaaac caggaataat gtgttccagg
    38161 ctgtgggcat agagacgccc gccggtgacg ttcttggcac ctgcggaatt gccacgctcg
    38221 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    38281 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    38341 caggaccaaa atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagcgccg
    38401 gaaggatctt cacggcgtcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    38461 ttttatcttt gttgatggcg aaaatggttt gcgatgcatt agcaccaacc atgtgctgga
    38521 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    38581 cgacatagcg ttcgtgctcc atccattttt cgttttccgc caccggacga gaacaggcca
    38641 actccgcacc tatcgccttg caaagctgtt ctgccagcgc aatgttctct ttgctgccaa
    38701 taccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    38761 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    38821 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtaccgctg ctgatggtca
    38881 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccaccg taaaccatgt
    38941 gtttcactgt cgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    39001 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcagca
    39061 gaaccaggcc gtctgcgccg tgctggcgaa tagtgtcagc catgacactg gcgtaatctt
    39121 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    39181 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    39241 gcgcaccgtt catcagttcc ggcagacgag aaggggtatc gctgaatacc cagacttgag
    39301 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    39361 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    39421 cgcagcaacc tgttgttcag accaggcgtc cacggcgtta aaaccgatat ccgccgccga
    39481 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    39541 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    39601 ttcatcttcc agttcgcgct caacggtgag ggtatctgca gtcagggaga taattttgct
    39661 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    39721 ataaaggtcg gaagaaccat cgccacagag gatcaaatca aagcctgctt tctgggcggc
    39781 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    39841 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    39901 ggctttaccg cccacactta aggccgtcac ctgcgcctct gctgcctgtt gctttagctg
    39961 gcaagccgct tcaatagcgt tcagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    40021 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    40081 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    40141 ggtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    40201 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    40261 aaaacatggc gtagcaaaaa gaaattttca atattgcttt atggatcacc aatattgaaa
    40321 gtcagatgct atttttttac tcacaacaca acataacaaa ctgattaata atcaaaatta
    40381 acgagaaaag cgccctctat tcacccgaaa caaaaatgtg ataacaatca cagaatgcag
    40441 cttattgaat acccattatg agttagccat taacgcgtcc acgaggttaa taataattat
    40501 attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg ttttcacagt
    40561 ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag gttttctttc
    40621 cgccgttaat aatcgtcggc atactttgtt ggcttacagt cagagatctg gatgcagcga
    40681 atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg gcatttgaat
    40741 ggtatatggt ggtgatgctt ttcggttggt tctggctggt gtttggcccg tatgccaaaa
    40801 agcgtttagg taacgaacca cctgaattta gtaccgccag ttggatcttt atgatgttcg
    40861 cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac tactacatct
    40921 ccaccccgcc gtttggctta gaaccgaact cgacaggggc gaaagagttg gggctggctt
    40981 acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt tcagtcgcct
    41041 tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcg acactggtgc
    41101 cgctggtagg tgaaaaacac gccaaagggt tgttcggcac tatcgtcgac aacttctatc
    41161 tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg ctggtgaccg
    41221 agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct atcatcatta
    41281 cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta
    41341 tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg ttcattgtca
    41401 gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg ctgatgtatc
    41461 tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg cagggctgga
    41521 ccgtgttcta ctgggcatgg tgggtgattt atgctatcca gatgagtatc ttcctcgccc
    41581 gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg catggtgctg gggctgacag
    41641 cgtcaacctg gatcctgtgg actgtactcg gtagtaacac tctgctgttg atagataaaa
    41701 acattatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc attatcgaaa
    41761 cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc ctctgcttta
    41821 ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtcc acttgccgcg
    41881 aagtacgcga tggtgaagaa ccaccgctgc tggtgcgtat cggttggtca attctggttg
    41941 gcattatcgg tattgttttg ctggcgctcg gcggcctgaa accgattcaa actgccatta
    42001 tcgccggagg atgcccgctg ttcttcgtca acattatggt gacgctctcc tttattaaag
    42061 acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt tgaaagatgg
    42121 attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa ctgatggcca
    42181 gcgaaaactg ggaggcctat tttaccgagt gcgaccgtga cagcgtctac ccggaacgtt
    42241 ttgtcaaagc actggcggat atgggtatcg acagtctgct gatccctgaa gagcacggtg
    42301 gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggagctggga cgtctggggg
    42361 caccaaccta tgtactgtac cagttgccgg gcgggttcaa caccttcctg cgcgaaggca
    42421 cacaagagca gatcgacaag attatggctt tccgcggcac cggtaagcag atgtggaact
    42481 cagcgattac cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg acttataccc
    42541 gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagc agcgcctaca
    42601 ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct gtctacaccg
    42661 aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgaa aagctcggtc
    42721 tgcgtatgga tagctgctgt gaaatcacct ttgacgacgt ggaactggac gagaaagaca
    42781 tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac catgaacgtt
    42841 tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat gcggcgcgct
    42901 acgccaacca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg attcaggaaa
    42961 aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat gaagcagcgt
    43021 ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa tacttctgcg
    43081 ccaatgcggc atttgaagtg gtggatagcg caatgcaggt gctgggcggt gtcgggattg
    43141 cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc gtctccgggg
    43201 gatctgacga aatgcagatc ctgacgctgg gtcgtgcggt gctgaagcaa taccgctaag
    43261 tcaatgcgct gcctgatgcg acgctgatgc gtcttatcag gcctacggga gcacgaatgt
    43321 aggccggata aagcgtttac gccgcatccg gcagtcatgc gctacataaa tttttcagga
    43381 gcaaataatg gatcatctac ccatgccgaa attcgggccg ttggccggat tgcgcgttgt
    43441 cttctccggt atcgaaatcg ccggaccgtt tgccgggcaa atgttcgcag aatggggcgc
    43501 ggaagttatc tggatcgaga acgtcgcctg ggccgacacc attcgcgttc aaccgaacta
    43561 cccgcaactc tcccgccgca atttgcacgc gctgtcgtta aatattttca aagatgaagg
    43621 ccgcgaagcg tttctgaaat taatggaaac caccgatatc ttcatcgaag ccagtaaagg
    43681 tccggccttt gcccgtcgtg gcattaccga tgaagtactg tggcagcaca acccgaaact
    43741 ggttatcgct cacctgtccg gttttggtca gtacggcacc gaggagtaca ccaatcttcc
    43801 ggcctataac actatcgccc aggcctttag tggttacctg attcagaacg gtgatgttga
    43861 ccagccaatg cctgccttcc cgtataccgc cgattacttt tctggcctga ccgccaccac
    43921 ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt aaaggcgaaa gtatcgacat
    43981 cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc atgatggatt acttcaacgg
    44041 cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc tactacgccg gttgcggtct
    44101 gtataaatgc gccgacggct acatcgtgat ggaactggtg ggcattaccc aaattgaaga
    44161 gtgctttaaa gatattggcc tcgcacatct actcggtacg ccggaaatcc cggaaggcac
    44221 tcagcttatc caccgtatcg aatgccctta cggcccactg gtagaagaaa aactcgatgc
    44281 ctggctggcg gcacatacca tcgcagaagt taaagaacgc tttgccgaac tgaatatcgc
    44341 ctgtgccaaa gtactgaccg taccggaact ggaaagcaat ccacagtatg tggcccgtga
    44401 atcaatcact cagtggcaaa cgatggatgg tcgcacctgc aaagggccga acatcatgcc
    44461 gaaattcaaa aataaccccg gacaaatctg gcgcggaatg ccctcacatg gcatggacac
    44521 ggctgccatt ttgaaaaata tcggctacag cgaaaacgac attcaggagt tggtcagcaa
    44581 aggtctggcc aaagttgagg actaaacgcg tggcgtcagt cggcttgcct gactagcgcc
    44641 cgcccaataa caaacgaaca aaaatggata gaggtgcaat ggatatcatt ggcggacaac
    44701 atctacgtca aatgtgggac gatcttgcgg acgtttacgg tcataaaacg gcgctgattt
    44761 gtgaatccag cggcggagtc gttaaccggt atagttatct tgagttaaat caggagatta
    44821 accgcacggc aaacctgttt tatacgctgg ggattcgcaa aggcgacaag gttgcactac
    44881 atctcgacaa ctgcccggaa tttatctttt gctggttcgg gctggcaaaa attggcgcga
    44941 ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag cgcgtggatc ctgcaaaata
    45001 gccaggcgtg cctgctggtg accagtgcgc aattctatcc tatgtatcaa cagattcagc
    45061 aggaagatgc cactcaattg cggcacattt gcctgacaga tgtggcactt cccgctgatg
    45121 atggcgtgag ttcgtttact caactgaaaa atcaacaacc tgccaccttg tgctatgcac
    45181 cgccgctatc gactgacgat acggcggaaa ttctcttcac ctccggcacc acctcccgac
    45241 cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc tggatattac tccgcctggc
    45301 agtgtgcact gcgtgacgat gacgtctacc tgacggtaat gcctgcgttt catatcgatt
    45361 gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga
    45421 aatacagcgc ccgcgccttc tggggacagg tgcagaagta ccgcgccacc attaccgaat
    45481 gtattccgat gatgatccgt acgttgatgg tgcagccgcc ttcagcgaac gatcggcaac
    45541 accgcctgcg ggaagtgatg ttttatctca acttgtcgga gcaggaaaaa gatgcgtttt
    45601 gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat gacggaaacc attgtgggca
    45661 ttatcggcga tcgccctggc gataaacgac gctggccgtc gattggtcgg gcggggtttt
    45721 gctacgaagc ggagatccgc gacgatcaca atcgcccgct cccggcaggt gagatcggtg
    45781 aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa agagtatttt ctcaacccga
    45841 aagccactgc gaaagtgctg gaagccgacg gctggctaca taccggcgat accggatact
    45901 gcgacgaaga gggctttttt tatttcgtcg atcgccgctg caatatgatc aaacgcggtg
    45961 gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc cacccatcca aaaattcagg
    46021 atatcgtggt tgtgggtatt aaagattcga ttcgcgatga agccatcaaa gcatttgtgg
    46081 tggtgaatga aggtgaaaca ttgagcgaag aggaattttt ccgcttctgc gaacaaaata
    46141 tggcgaaatt taaagtgccc tcttatctgg agatcagaaa agatctgcca cgtaattgct
    46201 cggggaaaat aattagaaag aatctgaaat aaatgaaaca gcagggaact accctaccag
    46261 ctaataatca cgctataaaa caatatgcat tttttgcagg gatgttgtcg tccctgaaaa
    46321 agcaaaaatg gagaaaagga atgagtgaat cattacatct gacccgcaat ggatcaattc
    46381 tggaaattac ccttgatcgt ccaaaagcga atgctattga tgcaaaaacc agctttgaaa
    46441 tgggcgaagt atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc attattaccg
    46501 gtgccggaga gaagttcttt tccgcgggct gggatttaaa agcggcagca gaaggcgaag
    46561 caccggatgc tgactttggc ccgggtggat ttgcgggatt aaccgaaatt ttcaatctcg
    46621 acaaaccggt tatcgcagct gtgaacggct atgcctttgg cggcggtttc gaactggcgc
    46681 tggcggcaga ttttattgtt tgtgccgata acgccagctt cgccctgccg gaagccaagc
    46741 tgggcatcgt tcctgacagc ggcggcgtgc tgcgtctacc gaagatcctg ccgcctgcca
    46801 tcgtcaatga aatggtgatg accggcagac gaatgggcgc agaagaggcg ctgcgttggg
    46861 ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga taacgcccgc gaactggcgc
    46921 agcagctggt taacagcgcc ccgctggcga ttgcggcgct gaaagagatc ttccgcacca
    46981 ccagcgaaat gccggtagaa gaagcgtatc gctatattcg cagcggcgtg ttgaaacact
    47041 atccgtcggt tctgcattcg gaggatgcca ttgaagggcc gctggcgttt gccgagaagc
    47101 gcgatccggt gtggaaagga cgttaacaac cgtgagctat tacgcctttg aggggttaat
    47161 tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc gtcttgattg gcgatgtgat
    47221 tgtgggagcc ggtgtctaca tcggcccact cgcctcactg cgtggtgact acgggcggtt
    47281 gatcgtgcaa gcgggagcca atattcagga tggctgcatt atgcatggct actgcgacac
    47341 tgacaccatc gttggggaaa acggccatat cgggcacgga gcgatcctgc atggttgtgt
    47401 gattggtcgc gatgcattgg tcgggatgaa cagcgtgatt atggatggcg cggtcattgg
    47461 cgaagagagc attgttgccg ccatgagctt tgtcaaagca ggctttcacg gcgagaaacg
    47521 ccagttgttg atgggtacgc cagcccgcgc tgtacgcagt gttagtgacg acgagttaca
    47581 ctggaaacgg ttgaatacca aagagtatca ggatcttgtc gggcgctgtc atgcatcgtt
    47641 acatgaaaca cagccactga ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac
    47701 ggatgtgacg cctaaacggt gatcttgccg gatgcgacct ggttgctgga taagatgcta
    47761 caacatcgca tctggcaagc aggacgcccg gtatcaacga gataaaatta acgacgcata
    47821 ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag cggaacggca
    47881 ggaatacgcg gcgtctttct tacgccgact aatttatccc ggctgttatt gcgcaggcgc
    47941 gcgtaaattt gctcatcgat atcgaccact tttaccagtc gctggcactg gcatccccgt
    48001 ccttccagct tattagggat catcttaact tcgcagctta tttctgcgac ttccgacaga
    48061 atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat agagatctct
    48121 cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa gtagagtggt
    48181 ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag tctggctatg
    48241 actcgttttg aagcaattaa acaaggccat attaaaattg tggatatatc gatagtatgc
    48301 aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc
    48361 ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg tattacttat
    48421 tcgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg atctgcgcgt
    48481 gcatttcctg caccgaaatt actttttcgg tcgcgtcggc attcagcgcc atcgcggtag
    48541 cgaaaccacc gttcagggtg gtgtcataat gcactttata ttgcagcgca ctgcgacgga
    48601 tcacgcggga gtcttcaatc gcgcgacggc ctgaagtggt gttgatgatg taggtatatt
    48661 cgccattctt gatgcggtcc tgaatgtgcg gacggccttc atgcaccttg ttcaccagac
    48721 gcggattaat gcccgcttcg cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga
    48781 agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg ccttcgcgca
    48841 cggaaagcaa cgcacgaccg tgtttcttca tggtggagtt gctgcccagc tgcgctttgg
    48901 caaacgcttc agcgaaggtg cggcccacgc ccatgacttc cccggtagag cgcatttctg
    48961 gccctaacag cgggtcaaca cccgggaatt tattgaacgg cagcaccact tctttcaccg
    49021 agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat ttgccagcca
    49081 tcacacgcgc cgccactttt gccagcggaa cgccggtggc tttggagacg aacggaacgg
    49141 tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc accgcaaact
    49201 gcacgttcat cagaccgcgc acctgcaatt cgaaggccag tttctgcacc tgctggcgca
    49261 tcacatcctg aatttcctga cttaaggtgt aggctggcag agaacatgcg gagtcaccgg
    49321 agtgcacgcc cgcctgctcg atgtgctcca tgatgccgcc aatcagcacc atttcgccgt
    49381 cgcagatggc atccacgtca acttctaccg cgtcatcaag gaagtgatcc agcaacactg
    49441 gcgcatcgtt agacacgctg accgccgtct ggaagtagcg acgcaggtca gcttcgtcat
    49501 agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgtaccacc agcgggtagc
    49561 caatctcttt cgccttctct accgccattt caatagcggt aacggtggcg ttcgccggtt
    49621 gtttcagttt cagacgctca accgcatgct ggaagcgttc acggtcttct gcacggtcga
    49681 tagcatccgg gctggtgccg ataaccggta cgccagcagc ttccagcgcg cgcgccagtt
    49741 tcagcggggt ctgaccgccg tactgaacga taacgccttt cggcttctca atacgcacga
    49801 tttctagcac atcttccaga gttaccggct cgaagtagag gcgatcggaa gtgtcgtagt
    49861 cggtggagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca
    49921 gcgccagcga ggcgtgtaca cagcagtagt cgaattcgat accctgaccg atacggttcg
    49981 ggccgccgcc gagaaccatg attttttcac ggtcggtaga cggattcgct tcgcactctt
    50041 cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca caggtatcca
    50101 cgcgcttata aaccgggtgc aggtcatact ggtcgcgcag cttgcggatt tccgcttcgc
    50161 gcacgcccgc cagttttgcc agacgcgcat cggcaaagcc tttgcgtttc agctggcgca
    50221 ggaattcagc gttcaggcca gtgatgccca cttccgctac tttctcttcc agacgcacca
    50281 gttcttcaat ctgtaccagg aaccagcggt caatattggt caggttgaac acgccgtcca
    50341 cagacaggcc cgcacggaag gcatcggcga tgtaccagat acgctctgcg ccagcatctt
    50401 tcagttcgcg acggattttg gttaacgctt ccgggtcatc gaggctcact ttcgggtcga
    50461 atccagtcgc accgacttcc aggccgcgca gcgctttttg cagggattcc tgctgcgtgc
    50521 gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg tcgttagcac
    50581 cagcgaattt ttcgaagttg aagcgaggaa ttttagtaac cacatagtcg atggacggct
    50641 cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat cagttcgtcg agggtgtaac
    50701 ccaccgccag tttcgccgcc actttagcaa tcgggaaacc ggtcgctttc gacgccagcg
    50761 ccgaagaacg ggacacgcgc gggttcattt cgataacaat caggcgaccg tttttcgggt
    50821 tcaccgcaaa ctggacattg gaaccaccgg tttcaacgcc gatttcacgc agcaccgcca
    50881 tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct ggcgcgacag
    50941 tgatggagtc accggtatgg atgcccatcg catcaaagtt ttcgatagag cagacgatga
    51001 tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag ccgatcagcg
    51061 actcatcaat cagcagctct ttggttgggg agagatccag accgcgggcg caaatttctt
    51121 caaactcttc gcggttataa gcgataccgc cgccgctacc gcccatggta aaggatgggc
    51181 gaataatgca cgggaagccc acgtcagcgg caaccgccag cgcttcttcc atcgtgtgtg
    51241 cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg aaacgacggc
    51301 ggtcttctgc tttatcaatc gcatcggcgg tcgcaccaat catagtcacg ccgaactctt
    51361 ccaacacgcc ctgacgctcc agctccagcg cgcagttcag cgccgtctga ccgcccatcg
    51421 ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtactact tcccagtgaa
    51481 tcggctcgat gtaggtcgca tcggccattt ccgggtcggt catgatggtc gccgggttgg
    51541 agttcaccag aatgacgcgg taaccctctt cgcgcagggc tttacatgct tgcgcgccag
    51601 agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga atcaggatac
    51661 tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag cggttttacg
    51721 gtactgctca attaactcga taaagtggtc gaacaacggc gcggcgtcgt gtggaccagg
    51781 gctggcttca gggtgcccct ggaagctgaa cgccggttta tcggtgcgat gaatgccctg
    51841 taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgctggta atgttgcttc
    51901 gtccaccgca aaaccgtggt tctgggcggt gatcatcacc acgtttttct caacatcttt
    51961 aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg caccgctcgc
    52021 cagcgccagc agctgatgac cgaggcagat gccgaagacc ggaatatcgg tttcgaggaa
    52081 tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac cgttggagag
    52141 gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg ccggaacgat
    52201 ggtcaggcga cagcctctgt ccaccaacat gcgcaggatg ttgcgcttgg caccaaaatc
    52261 ataagccacg acgtggaacg gcagctcgtc ttcttttttc gcttctggca ggccgccggt
    52321 caacgtccag ctcccttgtg tccagctata ggcttctgcg gtggtcactt cttttgccag
    52381 atccatgcca ttcagacctg ggaacgcgcg ggctttttct aacgccaacg ccgcatccgg
    52441 gttatcgcct gcgataatgc agccattctg tgcgcctttc tcgcgcagta aacgtgtcag
    52501 cttacgggta tcgatatcgg caatcgccac gatgttatgg cgcttcaggt aagaagagag
    52561 gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca ccagaccttg
    52621 tgcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc caatatgggg
    52681 ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt cttgataacc
    52741 ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc ctatggcccg
    52801 accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca aaacaccctc
    52861 cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt aaatcaatgc
    52921 aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat gggcgctaaa
    52981 agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt tttacaggca
    53041 ttaagcggca aaaagatcat ataacctgtt ctggaaaacg cttgcgcagc aagaagattg
    53101 catgaaataa aagcgagtag ggaaaagtgg accaaatggt caaaatttac attaaaacaa
    53161 caaaaaccga agacaaaaca tgtaattttt tgcacttata gacaatttaa aaacataaca
    53221 ctaaaaataa aagggccaaa aattaaagcc ctttacaatc aataaattaa atgtgttatt
    53281 tttgcaccat aacaaatatt ttgtggttac aaactattga gatcaagcac atctcgcata
    53341 tcaaaaagac cactttcctt accactcaac cacaaagccg atcttaccgc gccgttagca
    53401 aatgtcatgc ggctggacgc cttatgggtg atctccagac gctcgccaat atcggcaaac
    53461 atcgcggtat gttcgccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca
    53521 ggcacacgtt caccggtgtg gccttcacga ctgtagaccg cgcaatcttt cagatcttta
    53581 tcaagggcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact
    53641 ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcacccat cactttggct
    53701 gctttctcca gcagcttaag catgacgtta acgccaacgc taaaattggc ggcaaagaca
    53761 atcgcaatat cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta
    53821 gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct
    53881 tccggacggg taaaatcgat aaacacatca aaatcatctt ttaccgcatc gaggctgctt
    53941 tgcacggtaa cgcctgtttt ccctgcacca gccagctcac cggcgtcgct gcccagtaaa
    54001 gaagatcctt cgcgctccag cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc
    54061 tgaatcaact ggcggcccat acgccccccg gctcccgcga tggcaacgcg gatgtttgca
    54121 tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca
    54181 tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat
    54241 atcagtgaaa ggcatgagtc gatgaatgac tgcatgccga taacatgtga caggttacga
    54301 cgccagagcc agcacctcag ccacccactg ctggaagcct ttcacatcca gatccaatgc
    54361 cacctttaca ttggctggtt tgcccaggca accgtcgata tcaaccaccg tcgtgcctga
    54421 ggtaaattcg ccttgagttt ctaccgcgac aaaacacggc ttgagggtga ataagtccgg
    54481 gcgcaccagc caggcgatgg cgcagagatc gtgcattcgc agaccgcttt gcatactgcc
    54541 gctacggtag tggctaaaca gtgcatgaag catttttccg gtgcgattca actccggcaa
    54601 agtagcgaga tagtcagggg ttaatattgc ctgattggtg acatccagac cgcacatgac
    54661 gatttcgatt ccgctacgga agacacaggc agccgcttct ggatcggcag cgatattaaa
    54721 ctcggcgttt ggcgtacagt tgccgcgtcc ggcagaacca cccatgatca ccagacggcg
    54781 aatatacggc ttgcattccg ggcattgtga aagtaacagc gcaatattgg ttaacgggcc
    54841 aatggccacc agggtaacag gctctggtgc acgcatcagg gcatcccgaa tcgccagaaa
    54901 cgccggtatc ccgagcggct ttcggttgtg ctcaacaaag tcgtagccag ccattcccga
    54961 ttcgccgtgc acagatgccg catcacgcgg tgcgcgtacc agcggcacag cggccccttg
    55021 ggcgagcgga atctccgcat tccagaaatg cagcagttgc agggcattgc gggtagtttt
    55081 ctcaaccgag acattacccg cgacggtggt catcagttgc aggtcgagtt cgggtgcaaa
    55141 aatcgcggcg gcaatggcga cggcatcgtc aatgccgggg tcagtatcga ggaagatagg
    55201 taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt
    55261 aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt cggcacttcg
    55321 aaaacaatgt tttcttcacg tccttccagc ggaatggctt caccaccgcc cagctgttgc
    55381 aaacgtgcca ccacattctg caccagaata tccggagccg atgcgcccgc agtcacgccg
    55441 acgcatttaa cctctttcac ccactcttcc tggatatctt tcgcatcatc aatcaaaaac
    55501 gcgcttttgc ccatacgctg ggccagctcc gccagacggt tggagttaga ggagtttttc
    55561 gaaccgacca ccagcacaac ttccgcctgt tcggccaggg cacgtaccgc ttcctgtcgg
    55621 ttagtcgtgg cgtagcagat gtcatctttg cgcggaccga caattttcgg gaagcgttta
    55681 cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtctg ggtcataaag
    55741 gagagtttct cttcgttttt gaccgtcagt ttccacacat cgtctggtga ttcaaccaga
    55801 tacattcccc cttccgggtt gctgtactga cccatcgtgc cttccacttc cgggtgcccg
    55861 gcgtgaccga tgagaataga ttcttcgcca cggcgactag cgcgggcgac ttccatatgc
    55921 actttggtca ccagcggaca ggtcgcgtca aataccgtca aatcgcgact ttttgcttcg
    55981 ttacgtaccg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact
    56041 tcgctaatct gctcaataaa gatagcccca cgctcacgca agctatcgac cacatagcgg
    56101 ttatgtacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag cgcgttttca
    56161 acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc caacaggatc
    56221 tgcatgttac gcctccagtg ccggatcgat ttccagcact tcaatatcaa aatgaacggt
    56281 ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat
    56341 cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc caatttctgg
    56401 ctcgcctgca tccataaatt cacggcggga gaagtactga atcaggtccg gtgacggcac
    56461 gccaaacgcc gcatctggct ccaacgagaa ggtggtttta tcgcccactt tcagccccaa
    56521 caggtgttgc tccagccctt cagaaagaga agcatcaccc aggcggaaca gcgccggttt
    56581 accgttgttg cgggtagact cggcggtggt gccatcgtcg agttttagcg tgaagtgcac
    56641 caggacggcg ctattgctct gtacagattc agacatgcag gtttgctctt atattttgag
    56701 gatttttgcc ggatgcggcc tacgatttcg cagcaatctg taggccttat ccggcagggt
    56761 ttattattgt tttttcgctt tagaaggcaa aaaaccttcc agcacaatca gtgccgcacc
    56821 gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa
    56881 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc
    56941 gccaataatc agcgcataag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    57001 catcactgcc aggatcacgc taataccaat cgcaataccg gcaaagaacc aacgctgcca
    57061 gccgccgcta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    57121 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    57181 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    57241 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    57301 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    57361 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagt
    57421 acttcgctct gctgagcatc agcaggtgcg tcgttatagt ctgcaacggt agcgccggag
    57481 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccga cagttccggt
    57541 tctgcataca aggttactgc cgcttccagc gagccaccca ctttcttgtc ggcacgcgct
    57601 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    57661 ttcatcgctt cactgtctgc cagaccaaac aggccttcgt accactcacc ggtaaagacg
    57721 tatttttcac gttcgcccgg caggtagccc cacacttcat cagcggtgaa ggagaggatt
    57781 ggtgccatcc agcgcaccag cgcttctgcg atgtgataca gcgcagtctg gcagctacga
    57841 cgcgccacac tgtccgcttt ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag
    57901 gaacccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    57961 gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg cacaacctac ggcccagcga
    58021 tccagtacca ccatctcttc cggtttcacc atatcttttg ccggatcaaa accgttcagg
    58081 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctgtcggc agcacgtttc
    58141 aggatctcat cagaaacggc catttcaccg gtgtagtcag ttgatgccac ccacagacgc
    58201 agaatatccg cgcccagttt gttcatcaca tcctgcggcg aaacggtgtt gccgatggat
    58261 ttagacatct tgcgaccctg accatccacg gtaaagccgt gggtcagtac ctgacgatac
    58321 ggcgctttgc ctttcatcgc ggtggagatc atcagggaag acatgaacca accacggtgc
    58381 tggtcagaac cttccagata catgtccgct gcgtgaccgg caaattccgg acgcacgtca
    58441 acaacagaag agtgggtaga tccggagtca aaccatacat ccaatgtgtc cggcactttc
    58501 acgtactgat cagcttcatc gccgaggatc tctttcgcat caagatccca ccacgcctgg
    58561 atgccgtcaa cttcaacgcg ttttgccact tcttccatta gttcgagagt acgcggatgc
    58621 agctcttccg tgtctttgtg cacgaacagt gacatcggta cgccccaggt gcgctgacgg
    58681 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtcc
    58741 gggatccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    58801 atgctgacga accactgcgg cgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag
    58861 cacggatagc tgtgctgcat tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc
    58921 gcaacgacga tgtcgttcgc tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc
    58981 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccaggccgta tttctgaccg
    59041 atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa cggcaccggt accggcatcc
    59101 agggtaacgt gatcgccgag gattgccgga acgtcgaagc ccataaacgg atgggtaaag
    59161 cgcagcaact caagctccgc accttttacc gtgccgagaa tggtgtaatc ggtcacgccg
    59221 atacgctgca ttacgctttc aaccagatct ttcgccagaa tcacggcctg accgtcgatc
    59281 tgcaccagcg catagtcgaa atcaggtgca atagagattg cgcggttcgc aggcagagtc
    59341 cacggcgtag tggtccagat caccagcgag attgggccgt taacgttgct tacggcaaat
    59401 tttgctttca gtgcatcctg atcgaccgcc tgaaaagcaa cgtcgatgga cggagaagtt
    59461 ttgtcgtaat actcaacttc cgcttccgcc agcgcagaac ggcagtcaac gcaccagtga
    59521 actggcttcg cgcctttgtg caggtgaccg ttgccgatga ttttgcccag cgcgcggatg
    59581 atgttggctt cagttttgaa gtccatggtc aggtacgggt gcgaccagtc gcccagcacg
    59641 cccagacgga taaagtcttt gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac
    59701 ttggcgcgga actcggcggc ggtgaatttc tcacccggct taccgtattc ttgctctact
    59761 ttcagctcga tcggcagacc gtggcagtcc cagccaggca catacggcga gtcataaccg
    59821 gaaagccctt tggacttcac gataatgtct ttcagaatct tgttaaccga gtgaccaata
    59881 tgaatgctgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    59941 ttagccgcac ggatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    60001 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    60061 ttatagtcac tcatcagatt ctcggttccg tgtttcggtt tgattacata acaggcttaa
    60121 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    60181 ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    60241 tgtatatggc gaccgtaaag gtccattgca acatctaaca aatgcacttc cagctgctgg
    60301 cgaataccgg caaccgttgg gcgtgttcca atgtttgcca cgccgggtaa cggcttttca
    60361 ccgaggccca gcacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    60421 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    60481 ccggagatgg caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatccgcc
    60541 agcgcctgac gcacagcggt gctgctgata cgcacgccac cttcgcaaaa agtttgcgta
    60601 ctggtgatat cgaagccgta ttccatgcca gctttctgta ataacaagaa atcgccttca
    60661 cgaccagcgc caaagcggaa atcatcacct acggcaagaa attttacgcg caaatgcttc
    60721 accagaagat cgctgatgaa attttgcgcg gttaacgccg cgaaacgcct gtcgaaacgc
    60781 acgcacagca cgtaatcaac gccacactct gcaaggtaac gcagtttttc ccgcagccgg
    60841 gtcagccgtg ccggggcttt atcggtagca aacagttcca gtggttgagg ttcaaaaagc
    60901 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    60961 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    61021 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    61081 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    61141 aaagaagtgg cttttcattg ccatggcgca aatcacggga agaaagtgac cgcctgctgc
    61201 aatttttatc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    61261 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    61321 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    61381 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    61441 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    61501 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accgatcgtg
    61561 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    61621 ctgactgcac agatcaacaa actggcttaa tcgccaattt gctgaagctt tgtgaaaaag
    61681 cccgcgcaag cgggtttttt tatgcctgct gcttttatga tgcgtgagag aacacactac
    61741 cggttgccag atagtcaaaa gatgctggct tttgcagaca tgccgttaca ctttctgaac
    61801 tcttcgtggc ttaagaatcg tagaaccatc ggctgataca ttctgaacaa gaatatctac
    61861 tcaatctcca gactccccta caaagaatgt ggatattgtg atacacattg aggtattaaa
    61921 cttaagacca ctct
//
LOCUS       DAMZII010000011        59752 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZII010000011 DAMZII010000000
VERSION     DAMZII010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.011"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(231..407)
                     /locus_tag="POA77_000655"
     CDS             complement(231..407)
                     /locus_tag="POA77_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDI6364140.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            564..1412
                     /locus_tag="POA77_000656"
     CDS             564..1412
                     /locus_tag="POA77_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDI6364141.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQNYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            1409..1888
                     /locus_tag="POA77_000657"
     CDS             1409..1888
                     /locus_tag="POA77_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDI6364142.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1942..2892)
                     /gene="corA"
                     /locus_tag="POA77_000658"
     CDS             complement(1942..2892)
                     /gene="corA"
                     /locus_tag="POA77_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI6364143.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            3262..4026
                     /locus_tag="POA77_000659"
     CDS             3262..4026
                     /locus_tag="POA77_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDI6364144.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(4173..6335)
                     /gene="uvrD"
                     /locus_tag="POA77_000660"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(4173..6335)
                     /gene="uvrD"
                     /locus_tag="POA77_000660"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDI6364145.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(6419..7135)
                     /gene="yigB"
                     /locus_tag="POA77_000661"
     CDS             complement(6419..7135)
                     /gene="yigB"
                     /locus_tag="POA77_000661"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDI6364146.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(7135..8031)
                     /gene="xerC"
                     /locus_tag="POA77_000662"
     CDS             complement(7135..8031)
                     /gene="xerC"
                     /locus_tag="POA77_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI6364147.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(8028..8735)
                     /locus_tag="POA77_000663"
     CDS             complement(8028..8735)
                     /locus_tag="POA77_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDI6364148.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8732..9556)
                     /gene="dapF"
                     /locus_tag="POA77_000664"
     CDS             complement(8732..9556)
                     /gene="dapF"
                     /locus_tag="POA77_000664"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDI6364149.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9593..9796)
                     /locus_tag="POA77_000665"
     CDS             complement(9593..9796)
                     /locus_tag="POA77_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6364150.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10146..10700
                     /locus_tag="POA77_000666"
     CDS             10146..10700
                     /locus_tag="POA77_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HDI6364151.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11068..12546
                     /locus_tag="POA77_000667"
     CDS             11068..12546
                     /locus_tag="POA77_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDI6364152.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKRTDNNGGGNRL
                     DIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQKSWDQRGKFST
                     AFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            12543..13856
                     /locus_tag="POA77_000668"
     CDS             12543..13856
                     /locus_tag="POA77_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDI6364153.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMYRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIPLDTP
                     DLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            13904..14224
                     /gene="cyaY"
                     /locus_tag="POA77_000669"
     CDS             13904..14224
                     /gene="cyaY"
                     /locus_tag="POA77_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDI6364154.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(14264..16810)
                     /gene="cyaA"
                     /locus_tag="POA77_000670"
     CDS             complement(14264..16810)
                     /gene="cyaA"
                     /locus_tag="POA77_000670"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDI6364155.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            17197..18138
                     /gene="hemC"
                     /locus_tag="POA77_000671"
     CDS             17197..18138
                     /gene="hemC"
                     /locus_tag="POA77_000671"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI6364156.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            18135..18875
                     /gene="hemD"
                     /locus_tag="POA77_000672"
     CDS             18135..18875
                     /gene="hemD"
                     /locus_tag="POA77_000672"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI6364157.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            18897..20156
                     /gene="hemX"
                     /locus_tag="POA77_000673"
     CDS             18897..20156
                     /gene="hemX"
                     /locus_tag="POA77_000673"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6364158.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPAPAPQGDTPAAAPQGE"
     gene            20159..21355
                     /gene="hemY"
                     /locus_tag="POA77_000674"
     CDS             20159..21355
                     /gene="hemY"
                     /locus_tag="POA77_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDI6364159.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            22034..22195
                     /locus_tag="POA77_000675"
     CDS             22034..22195
                     /locus_tag="POA77_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364160.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(22279..23514)
                     /locus_tag="POA77_000676"
     CDS             complement(22279..23514)
                     /locus_tag="POA77_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI6364161.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLGDETAASDGLKLH
                     APGDIQGELKEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(23661..23737)
                     /locus_tag="POA77_000677"
     tRNA            complement(23661..23737)
                     /locus_tag="POA77_000677"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23701..23703),aa:Pro,seq:tgg)
     gene            complement(23780..23866)
                     /locus_tag="POA77_000678"
     tRNA            complement(23780..23866)
                     /locus_tag="POA77_000678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23830..23832),aa:Leu,seq:cag)
     gene            complement(23887..23962)
                     /locus_tag="POA77_000679"
     tRNA            complement(23887..23962)
                     /locus_tag="POA77_000679"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23927..23929),aa:His,seq:gtg)
     gene            complement(24021..24097)
                     /locus_tag="POA77_000680"
     tRNA            complement(24021..24097)
                     /locus_tag="POA77_000680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24061..24063),aa:Arg,seq:ccg)
     gene            complement(24200..25585)
                     /locus_tag="POA77_000681"
     CDS             complement(24200..25585)
                     /locus_tag="POA77_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6364162.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(25776..26516)
                     /gene="rffM"
                     /locus_tag="POA77_000682"
     CDS             complement(25776..26516)
                     /gene="rffM"
                     /locus_tag="POA77_000682"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDI6364163.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(26519..27871)
                     /gene="wzyE"
                     /locus_tag="POA77_000683"
     CDS             complement(26519..27871)
                     /gene="wzyE"
                     /locus_tag="POA77_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDI6364164.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(27868..28947)
                     /gene="rffT"
                     /locus_tag="POA77_000684"
                     /gene_synonym="wecF"
     CDS             complement(27868..28947)
                     /gene="rffT"
                     /locus_tag="POA77_000684"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDI6364165.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(28944..30194)
                     /gene="wzxE"
                     /locus_tag="POA77_000685"
     CDS             complement(28944..30194)
                     /gene="wzxE"
                     /locus_tag="POA77_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDI6364166.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(30196..31326)
                     /gene="rffA"
                     /locus_tag="POA77_000686"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(30196..31326)
                     /gene="rffA"
                     /locus_tag="POA77_000686"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDI6364167.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(31331..32005)
                     /gene="rffC"
                     /locus_tag="POA77_000687"
                     /gene_synonym="wecD"
     CDS             complement(31331..32005)
                     /gene="rffC"
                     /locus_tag="POA77_000687"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDI6364168.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(31983..32864)
                     /gene="rfbA"
                     /locus_tag="POA77_000688"
     CDS             complement(31983..32864)
                     /gene="rfbA"
                     /locus_tag="POA77_000688"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDI6364169.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(32883..33950)
                     /gene="rffG"
                     /locus_tag="POA77_000689"
     CDS             complement(32883..33950)
                     /gene="rffG"
                     /locus_tag="POA77_000689"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDI6364170.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKAGSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(33950..35212)
                     /gene="wecC"
                     /locus_tag="POA77_000690"
     CDS             complement(33950..35212)
                     /gene="wecC"
                     /locus_tag="POA77_000690"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDI6364171.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(35215..36339)
                     /gene="wecB"
                     /locus_tag="POA77_000691"
     CDS             complement(35215..36339)
                     /gene="wecB"
                     /locus_tag="POA77_000691"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI6364172.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILINPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRI"
     gene            complement(36395..37441)
                     /gene="wzzE"
                     /locus_tag="POA77_000692"
     CDS             complement(36395..37441)
                     /gene="wzzE"
                     /locus_tag="POA77_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDI6364173.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(37453..38556)
                     /gene="wecA"
                     /locus_tag="POA77_000693"
     CDS             complement(37453..38556)
                     /gene="wecA"
                     /locus_tag="POA77_000693"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDI6364174.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(38796..40055)
                     /gene="rho"
                     /locus_tag="POA77_000694"
     CDS             complement(38796..40055)
                     /gene="rho"
                     /locus_tag="POA77_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI6364175.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(40140..40241)
                     /gene="rhoL"
                     /locus_tag="POA77_000695"
     CDS             complement(40140..40241)
                     /gene="rhoL"
                     /locus_tag="POA77_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDI6364176.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(40382..40711)
                     /gene="trxA"
                     /locus_tag="POA77_000696"
     CDS             complement(40382..40711)
                     /gene="trxA"
                     /locus_tag="POA77_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDI6364177.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            40842..42107
                     /gene="rhlB"
                     /locus_tag="POA77_000697"
     CDS             40842..42107
                     /gene="rhlB"
                     /locus_tag="POA77_000697"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDI6364178.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            42241..43725
                     /gene="gppA"
                     /locus_tag="POA77_000698"
     CDS             42241..43725
                     /gene="gppA"
                     /locus_tag="POA77_000698"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDI6364179.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(43772..45793)
                     /gene="rep"
                     /locus_tag="POA77_000699"
     CDS             complement(43772..45793)
                     /gene="rep"
                     /locus_tag="POA77_000699"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDI6364180.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            46010..46459
                     /locus_tag="POA77_000700"
     CDS             46010..46459
                     /locus_tag="POA77_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6364181.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQISTAYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            46612..46893
                     /gene="ppiC"
                     /locus_tag="POA77_000701"
     CDS             46612..46893
                     /gene="ppiC"
                     /locus_tag="POA77_000701"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDI6364182.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(46939..48414)
                     /gene="ilvC"
                     /locus_tag="POA77_000702"
     CDS             complement(46939..48414)
                     /gene="ilvC"
                     /locus_tag="POA77_000702"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI6364183.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            48564..49457
                     /gene="ilvY"
                     /locus_tag="POA77_000703"
     CDS             48564..49457
                     /gene="ilvY"
                     /locus_tag="POA77_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDI6364184.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(49509..51053)
                     /gene="ilvA"
                     /locus_tag="POA77_000704"
     CDS             complement(49509..51053)
                     /gene="ilvA"
                     /locus_tag="POA77_000704"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDI6364185.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(51056..52906)
                     /gene="ilvD"
                     /locus_tag="POA77_000705"
     CDS             complement(51056..52906)
                     /gene="ilvD"
                     /locus_tag="POA77_000705"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI6364186.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52971..53900)
                     /gene="ilvE"
                     /locus_tag="POA77_000706"
     CDS             complement(52971..53900)
                     /gene="ilvE"
                     /locus_tag="POA77_000706"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDI6364187.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(53920..54183)
                     /gene="ilvM"
                     /locus_tag="POA77_000707"
     CDS             complement(53920..54183)
                     /gene="ilvM"
                     /locus_tag="POA77_000707"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDI6364188.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(54180..55826)
                     /locus_tag="POA77_000708"
     CDS             complement(54180..55826)
                     /locus_tag="POA77_000708"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDI6364189.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(55829..55879)
                     /locus_tag="POA77_000709"
     CDS             complement(55829..55879)
                     /locus_tag="POA77_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364190.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(55966..56064)
                     /gene="ilvL"
                     /locus_tag="POA77_000710"
     CDS             complement(55966..56064)
                     /gene="ilvL"
                     /locus_tag="POA77_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDI6364191.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            56417..57937
                     /locus_tag="POA77_000711"
     CDS             56417..57937
                     /locus_tag="POA77_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDI6364192.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(57962..58300)
                     /gene="maoP"
                     /locus_tag="POA77_000712"
     CDS             complement(57962..58300)
                     /gene="maoP"
                     /locus_tag="POA77_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDI6364193.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            58419..59258
                     /gene="hdfR"
                     /locus_tag="POA77_000713"
     CDS             58419..59258
                     /gene="hdfR"
                     /locus_tag="POA77_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDI6364194.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(59354..59429)
                     /locus_tag="POA77_000714"
     tRNA            complement(59354..59429)
                     /locus_tag="POA77_000714"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59394..59396),aa:Trp,seq:cca)
     gene            complement(59438..59514)
                     /locus_tag="POA77_000715"
     tRNA            complement(59438..59514)
                     /locus_tag="POA77_000715"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59478..59480),aa:Asp,seq:gtc)
     gene            complement(59569..59684)
                     /gene="rrf"
                     /locus_tag="POA77_000716"
     rRNA            complement(59569..59684)
                     /gene="rrf"
                     /locus_tag="POA77_000716"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        14842 a        15881 c        14992 g        14037 t
ORIGIN      
        1 caaaaagaat aagagatact ggatgtaagg caatctactt tgtattgatt tcattgggtt
       61 tgacactttt ttctattatc tctgcagcaa ttgaaggggt gagatgtgag tcattaagcg
      121 tccaactcaa cgtggcatgg atattattca caacaattat attatctgcg attccctcgg
      181 ctaaaatgtc gctgtcagca cactaactta ctcacagcga catcgtatag ttacagccag
      241 ttcttacgct taaagtacag atacggtgtc aggcccgcaa ggatcataaa gataatcgcg
      301 ccagggtagc caaagctcca cttcagttct ggcataaact caaagttcat gccatagctg
      361 gaggcaatcc gcgtaggaaa aagtttccta atcggtaaat tcaacatttt gttattaaca
      421 atgagcaaga ccaatcatta acgaggttct cttcagagat gtaatgaaaa aatgtgaaat
      481 gtacttcttt tttaaatatt tttttaccct agcatttgtt ctcgaattga cattgagctt
      541 aaatataacc aaggcaaatg caaatgaata acatattact tccgccaatt aatattccct
      601 gtacactttt cgaaactatt agtttattcg atgatttttc tgctgatgat atgcaatatg
      661 gtgatatggt agaacaagac tttctctcac tcggcctaag cgatatctca gcaaaagttg
      721 atccttacag acttattaaa taccatttcc ctggcccagg ttctattaat gttgctttta
      781 gtgcctcaag ttccggaaca aaaatatcac aacgtgaatg cactgatatt ttatttgcag
      841 aaatgaaaga actagcaaaa atgttttcct tttttggaca aaataaaaca cttatcgaag
      901 atctgattga acattttaga tatggaaatg gcagcaattt tcatagccaa caattaaatt
      961 tatcttttca tgaaaaaata aataaatatg gttataatag tccaatacga ataatcaaag
     1021 aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct cttatcctgc
     1081 aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca
     1141 gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa
     1201 gttttcaaaa ctacgctata ggatggagtg caacaattca ttttgtagca caggaccact
     1261 tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca
     1321 tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta
     1381 acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa ttatattatg
     1441 tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac agattttgta
     1501 tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc catggactga
     1561 cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc aacatcccct
     1621 accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct tcactgacta
     1681 taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa
     1741 tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt atcgtaacac
     1801 tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata aaatagagcg
     1861 agttttcaat ccgcatgact ttaaataaga caatttttag aaataagccg gataaaaaaa
     1921 tagctttatc cggcactcat attacagcca gttcttgcgc ttaaagtaca gatacggtgc
     1981 caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc acttcagttc
     2041 tggcataaac tcaaagttca tcccatagct ggaagcaacg agcgtcggcg gcaggaatac
     2101 cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga taaagcccat
     2161 cgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg gcagcaggga
     2221 ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta aacgcgcctt
     2281 gcgtaccagg aagttgagcg cacgctgggt atccatcaga cacaggcgaa ctttccagcc
     2341 gatatcttcc agttccgcca gcgtggagag cgcctcatcg tactcatcgc cctgatgccc
     2401 ttccataatt acccggctca actgctccag atcgctgtag atattttcaa tttcatctgc
     2461 cagctgttcg attttggttt cgaacagatc caacagcagc tcgtaggcgt taccgtccac
     2521 cattgactgg ctacgggcgc gcatacgata cagacggaat gcaggcagtt cacgctcacg
     2581 cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac cggcgtgatc
     2641 ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcatcgt cttcaaagaa
     2701 acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct ggccaagttc
     2761 agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt
     2821 tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt ccagttgaaa
     2881 tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac
     2941 agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg gaaaaattga
     3001 ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag ttgatcctgg
     3061 atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt
     3121 gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca gaacaccaca
     3181 gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca ggataaaaaa
     3241 atccaacatc caggacctat aatggcgcat cggctactca ttggtaaagg aatgatcacc
     3301 ttgaatctca aacggatttt tctcgccctc accttacttc ccttgtttgc tgttgccgct
     3361 gatgattgcg cactctcaga tccgacgctg accgtacagg cgtataccgt taatcctcaa
     3421 acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg aactttacat
     3481 gctctgctgg cggatatgaa tagtcagggt caggtgcaga tggcgatgaa cggcggcatc
     3541 tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca gaaggtggcg
     3601 ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt gttttatgtc
     3661 gcgggagata aagtcggcat cgttcgtctg gatgccttca aaaccagtaa agagattcag
     3721 tttgcggtgc agtcagggcc aatgttgatg gaaaacggtg taattaatcc gcgtattcat
     3781 cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca tgggaatgcc
     3841 gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg
     3901 aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat gtatatgaaa
     3961 ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt agagcggaaa
     4021 ggttaaaacg atagttttgt gtcagatact gaagatggcg cagatgccgg atgcagcgta
     4081 aacgccctat ccggcctaca tgacgttgca atttattgaa tatgaatgat tttttaggcc
     4141 aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg ggcgtatgcc
     4201 gccaccagcc atttgattcc ctggccctgg aacgccacct gcaaacggct atgttcaccg
     4261 ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac acgctgaccg
     4321 agcttgtagc cgctgtcgtt ctcgaccatc ggcgtcccca tccgttgatg gctgaccgga
     4381 cgacttaccg tagcgcgcag gcgcacctct tccacacact cttccggcag ctcgccgata
     4441 aagcgcgacg ggcgatggta aacctcttta ccatacagac ggcgggtttc cgcgtaggtc
     4501 agcgtcagtt tctgcatcgc gcgggttaca ccaacgtagg ccagacggcg ttcttcttcc
     4561 agacgcccgc cttcatccag cgacatctgg cttgggaaca taccctcttc cataccgaca
     4621 ataaacacct gcgggaactc caggcctttc gccgagtgca gcgtcatcaa ctgcactgca
     4681 tcctgccagg tatccgcctg cccctcgccc gcttccaacg ccgcatggga gaggaatgcc
     4741 tgcagcggca ttaaatcttc atcttcttcg ttgtagctga actggcgcgt tgccgtcacc
     4801 agttcctcta agttttcgat acgcgtctgg cctttttcgc ccttctcctg ctcgtacatg
     4861 gtgcgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg catatcggca
     4921 gtttcttgcg ctaaggcatc gattaattcc ataaatcgct gcaaggcgct ggcagctcgc
     4981 ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt taactggcga
     5041 tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg cgttggcgta
     5101 ttcaccacgc gctcaaacgc cgcgtcgtcg ttacggttgg caatcagacg cagatacgag
     5161 agcgcgtctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat acggtacggc
     5221 atactggcct gcaacagtgc ttcttccagt acacgcgact gggcgttact gcggtagaga
     5281 atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg gttaaccaca
     5341 aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc accgtccgcg
     5401 ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag ggcgttagcg
     5461 gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaatagt ttcggcaccg
     5521 gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca gccgtagatt
     5581 gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag caggcggatc
     5641 cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt ggtaaaacgt
     5701 tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc gcgcagcagc
     5761 agctcggcga agtccaccag gcccgcgcgg tcacacgctt cctgatacgc ctgatacacc
     5821 ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg acgcaggcct
     5881 tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg cttctcgtcg
     5941 aggttcatgg ctttgatcag acgcttaagc aggcgcagct ggtcctcgct atcgaggatc
     6001 tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg gtgcgccagc
     6061 ccgtggaagg tgccgaccca catgccgccc tggctggtgc ccatcagttg cccgatacga
     6121 tgacgcatct ccgccgccgc tttgttggta aacgtcaccg ccataatcga gtatggcgag
     6181 cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt cttaccactg
     6241 cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc gcgctgtttg
     6301 tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt
     6361 gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt
     6421 atatcagcga ggtcagagat gccaaccggg aaatttccag atgcggcagt aaacggctgt
     6481 cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc attccgctgc
     6541 gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg
     6601 gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt gagcgcccgt
     6661 gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt tccggctggg
     6721 cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt
     6781 gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc attgctgcgt
     6841 gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg
     6901 aacgaaaacg ccaacgcgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct
     6961 ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca
     7021 caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat
     7081 catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt
     7141 cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag
     7201 atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agcagctcct gcacaccacg
     7261 aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt tatgcgggtg
     7321 aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg
     7381 cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccaaacag
     7441 gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg
     7501 gcgctctttg ctgcctttcc ccataaccca cacttcacca gactccaggt cgaggtgttt
     7561 aatatccagc cccaccagct cagaaagacg cagacccgcg ccgtacatca cttccagcat
     7621 tgcacggtcg cgtacagcga ggggatcatt gatatcaatg tccagcagcc gattcatatc
     7681 gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa cacctttcgc
     7741 cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga
     7801 aagccgtaac gcgaggcttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt
     7861 gcgcaccatc gtcacatcac attgctgcca gctttgcagg ccgttttcgc tggcaaaatt
     7921 gatgatcgcc tcaagctgac gctggtagtt aagcagggtt atcgggctaa gctggcgctc
     7981 cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca
     8041 atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttcct
     8101 tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacgacacc caaatcagca
     8161 tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg tagcaccacc
     8221 agcagctctg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata
     8281 cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct
     8341 aagcgccagc gatccggaaa caggcgcaga ctcgcacctg ccagaccgag atcgcgcgcc
     8401 cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc
     8461 aggtagagta agcgataaaa caggccttcg ttggcgatag cctgttccat caacaacgcc
     8521 atgttctctt ccagaacatg aatatgatta cgtgcgcggg ccatgtgcca ttcgaccaac
     8581 gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattacgg
     8641 ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg
     8701 agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatccg tcgtagacat
     8761 gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac
     8821 gaccgccggg gagttccacg cgtacttctt cggccagcaa accttgctgg atccctaccg
     8881 ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa
     8941 cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg gctcgctccg
     9001 gaaaacgctc gtggctttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat
     9061 catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct
     9121 gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg
     9181 aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca
     9241 gaaccatccg cccgttggcg gtgctgacgc ggatatcgcg cttattggtc agccctttca
     9301 gacgcacaaa acgggcaaag cagcgcgcac cattgccgca ctgcgccact tcactgccat
     9361 cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca
     9421 gcagttggtc aaaccctact cccaggtgcc gatcagccag gcgacgaatc agctccggtg
     9481 aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca
     9541 ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat
     9601 tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg
     9661 tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga
     9721 gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aattaataca gtgagtgcct
     9781 taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc
     9841 gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga
     9901 aatgctgcga gtataacgct tacacgtggg gatttatgtt ttgctggtag tttcgtgatc
     9961 ggcacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt
    10021 tcattgttct tatcgtcggc atggggcttt taatagagtg gcgcaccatg caatttcgtt
    10081 gtctgacctt agtcgccagt gacatacaac tttgccaacc tggtgataag agggaataaa
    10141 atctgatggg gctatttacc caactggaaa aatttgacca aaagcttacg cgcggttatg
    10201 cccgctgggg gcgctgggta tggcggacgt taattgtcgt tccggtactg gtcgtgttgt
    10261 ggaatatagg acaggcagta tggggaggac caagaggtgg agtgattctg gaaatccaca
    10321 gtgaaattga ccgtccgata ctgggattca gtgttaacgg cgtggcgggc gcgaatgctt
    10381 ttgcaaatgg tggcggttca atcacctgtt gtggtgatgt cagtggggat acggcagagg
    10441 ttatctggac attaagtact actcgtacac agtataatgc tggaatgcgt ctggagaaac
    10501 ggaatatgac gttacctctc cccaaaagag aatggggaga agattttctg catgtgcatt
    10561 ttatgcctgg cgataaagtg ctgctggggt ggagtaaaga ttctttttct ccgtatgaag
    10621 atcttcacaa cggggggtac aagacccgcg ttcggcagga tgtgaaagat aaactttatg
    10681 gtacagggaa aatgaattga tagatttcta taaaaatcac gttcggcaga aagcgtaata
    10741 atgtggggaa tgttagatac cggtggtggg acctaagtac aacacgcgaa agacaataaa
    10801 gtaggtaacg gtgggcagag tcgcatgttg acacccttcc cggcgtcaat ccggcacatt
    10861 ttaacgtgat cagtcacacc atggcgcagt agctcgtatt tatatcatga atgaaaacct
    10921 caagtttacc ccagcaacta tcagaaaata gcgatggcct ttatagattc tgaaaagtga
    10981 cgatttgccg caaaagttac gcttacgtgc gaggaattat gttctgctgg tagtttcgtg
    11041 atcggcacct cgcgccagga tggctttatg cactatcgca atgtggtttg tttgttgtct
    11101 tgttcattgt ttttatcgtc ggcatgggga tgtcggcttg atccgcctga acataatatt
    11161 taccagaaac agggaaaagg cgtggtatac ctgcgccctt ataaagaaac aaatctttct
    11221 cttcctgacg tgaattacaa aagtttgcgc cgtttaccca atttattaat agacccgaca
    11281 acactagaca aatgggataa agtacctcct ctgactgatc tgacaaccga ctacctctat
    11341 gaaggcgcac aggcctggta tcctcattac tcctggcata gcgatggtcg ctatatcctc
    11401 tacgcgggtg aagttgtgca aaacccgcca gacaaacctc ccgtcgatgt cgcctcgttc
    11461 aaggcgtggg gtgattttgc cgcagataaa cacagtctct atttcgaagg caagcgcaca
    11521 gataacaatg gcgggggaaa tcgcttagat atcaaaacac tgcatcaggt ggaattccgg
    11581 cctccctggg atcctgactt actggggctg atattacgtg acgctaattt cttgtatatc
    11641 aatgggcatc gcctcgcaga tcccgatagt ttccgtgtgc tggcacaaaa gtcatgggat
    11701 cagcgaggaa agttttctac cgcgtttaat ccctgtgtgg ctatgccatt tggcccctgg
    11761 gataccctgg ctcgtacacg gaccaaaatc ctgatcaacg gcgaacagct tgatgccgac
    11821 ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg gcgtgataaa
    11881 aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga agatttaaca
    11941 aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa aggacctgct
    12001 tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat cagtgatgct
    12061 gtcgcccagt atcaggactc gctttatacc atcatcgaaa cagagtctgg tgaccgcaag
    12121 ctggaaatcg tgaaacttga tgatcccaat cttattatca acaaacgttt caacgccggg
    12181 aaacgccacg gctatttact tacgcgtgcc gaagggtggc cataccattc cggtttacat
    12241 gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga tgaacgcgaa
    12301 gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa ccgctacatt
    12361 tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa acaagttcgc
    12421 ttaatctgga aggaacaaca ttcgggatat ggctatggcg taaattacaa aacgggatat
    12481 ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa tgaggcgaca
    12541 aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca tttctgacgc
    12601 ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg tcttacggta
    12661 aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc agggccgatt
    12721 tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat gaaataagca
    12781 taaaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg cgtggcaaaa
    12841 tcgttagcaa tccaccggga acccccactg tcgatattgc cagctttcag gctatgggcc
    12901 gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa agtaatagcg
    12961 gcgcgtcacg tgttgatctg gcgacactaa aagctatcga aggtaactct accacgctgg
    13021 tggatagtaa aaatctctat ttgtccggtc gacgtcaggg gagcagcagt aatgttactg
    13081 tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac agaaatttgt
    13141 attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc gttcacctta
    13201 cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg ctggtttttc
    13261 gcgacaataa gggtatgtat cgttatccct ttggtcaatt atcaggcaaa gcgatacccg
    13321 tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt aaacagctca
    13381 cgtccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct gaacaatttc
    13441 atctgattac tggcaatatt gcgcaatata aagatcggct gtatgtaaca aaattatcga
    13501 tatttggtga agaccagctt gagataatcc cgctggatac gccagacctg gtcattgatc
    13561 gctcatttaa tggcggcaaa caacatgctt actttatccg ccaattacgg tcaaagagct
    13621 tgcaaattat tccagttaac ggtccgctaa ctaaaaacga tcgcttcgct tatgacgatc
    13681 gcaatgttta tacatggacc gatacagagg taaggattac gccctccccc tgcccggcga
    13741 aaactcgtgt cagagaggag aacgtacgtg aagttcaaaa cagagacatc attattccgg
    13801 tgacggatga atcatgccgg aacgcagcag cagaggtgca aactttgaag ccctgacccc
    13861 atgccgctat actgccgcca tcacttaaaa acaggataca acaatgaacg acagtgaatt
    13921 tcatcgcctg gctgatcaac tgtggctgac cattgaagag cgtctggacg actgggatgg
    13981 cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg accattacct ttgaaaacgg
    14041 cagcaaaatc attatcaacc gccaggaacc gctgcaccag gtatggctgg caaccaaaca
    14101 aggcggctac cattttgatc tgaaaggcga tgagtggatt tgcgatcgca gcggcgaaac
    14161 cttctgggat ttgctggaac aggcggcgac gcagcaggcg ggtgaaacag tcagtttccg
    14221 ctaagattgc atgccggata agcctcgctt tccggcacgt tcatcacgaa aaatattgct
    14281 gtaatagcgg cgtatcgtga tcctgattgg caggcggcat gttaccgata gattttgtgc
    14341 ggaacggaat cacctgttca cgaccatcaa ccttcacaat ctgatagaac tgcggcaggt
    14401 tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg cgatgacgag tagaagcgac
    14461 tgacgtcacg caccagttcc tctttgctgc cttcgcagtg gtgatacacc tcaacccggt
    14521 tgctttcgtc gagaatgtag atattaaagc cattctcgtc ttgcgtttct tcgaagaaga
    14581 actggatgat cccttcacta gcaaagccgt ccaccaccgc cggtaatttg acgtgattgg
    14641 tttcaacctg cactgacagg ccgtgcagtt tgttatgcga aatagcgcca taaaactcga
    14701 tggcgttctc cagcttctgt accgatacat tcaggcgttc gaagaacaag ccccaggttt
    14761 gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg gcgggtgctg gaaaggcgca
    14821 gttcaataca ctcagaaacc agttgctgca cgcgagtacg gattaacccg cgcagatgct
    14881 ggctataaca gaagacttcc acgctatctg gcggtgcggc gtcctgatgc attttgccga
    14941 gaatagtttt cagggcttcg atcatcgatt gctcgccgtt gaagtgcagc gtacgcactt
    15001 cgttccacga gttgcggtac agcaggtcaa cgctacctac caggcaattt tgattctcgc
    15061 caaagctgaa gacatccagc ttacggaaat caaaatgcac cacctgattg cggaacgccg
    15121 ctgtcgggtc atattccagg ttaacgataa tcgccagatg gcggatctca cacgggctgt
    15181 agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa atggtgcgac acgtcggcga
    15241 ccatctcctg caacttaggc aaatcgacaa tgccgttacc tttaatatac aaacgggtgc
    15301 gcgaggtcag caggccgtta aaccatgccc acgccaccag tttgttcagg taacggttat
    15361 attccagcgg ctgatggctg atgatcgact caatatttgg cgcgcggtta tacagatacc
    15421 aacctgaacg gttagcacgg cccggcggca cataaataaa ggtcagattc ggttccgaga
    15481 gatcgggtga aatctgcggg tttaccagcg tcactttacc tggtaatgct tcaaacgcgg
    15541 catacagctt acgcgtcagc acgccgatat cctgcggact ggcggagacg ctaaggttat
    15601 tgcgacgcgc aaagcggatc agattacggt agctctgcat catcgcgtcg agcaattcgt
    15661 tgtgcgcctc acgcacctga tcaatcttcc agttagcgcg gttatcgagc attgccagac
    15721 gagcttcgtc ccaaccccac tcgctcacta actggctcaa cactgcgcga cgccagccta
    15781 cacaggcgcg ttcacggctg agcttttcgc acacttttaa atagaagcag cgacgtacta
    15841 aatccagacg ggtaaaatct tcaatcgccg tcaggtattc agtaacacgc tccagcatca
    15901 tgcagtatgg atcgagacca aacgatacaa tctcgccgtc gtgcaaacgc tgtttgatat
    15961 ctttcgccag cagacgtggg ttcgggtatt cccaggaata ggcttccagc agcagtgttt
    16021 tcagtaccgc tttgtatggg gaatcgatac tcttgtagag ctgccaaagg ctggcaccaa
    16081 agtactcttc agcagaaagc gagcttaagc caccgagatc cagccattca tttggcgtca
    16141 gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta atgctcttct tcgtcgcacg
    16201 gcaccatatt ccacagaata cgcttaccgg cgagacgcac ggcggtacga taaaattcgt
    16261 caagcagcag tatatgctgg gtggagccac aatcttcgcc ccccaggctg ccgctttcat
    16321 tatgacggaa gcggttttca tcaatcagga agaagctgac ttccacaccc agcgaggcgg
    16381 cccagctttc cagcagacta catttacgtt gtagtaactg gcgctcttcg ctatcgagcc
    16441 aggattgatg acagacccag atatccaggt cagaggaaca actttgccct acggacgagg
    16501 tgctgcccat ggtgtataca ccagtaattg gaagctcacc tttcggtggg tcctgtactg
    16561 acattccacg atacagttca agctcgttca ggtagtggcg ttgagtttca tcaggcgtgt
    16621 aaaggcaaat gcctttggga acgttaccat caaggtaacc cggcattagc ggatggtgat
    16681 agtgcaacaa tgtcggcagt agactgtaga cctgttggaa tgcaggcccc atagcagcaa
    16741 gcgcgcgatc cacacgcaat tgatttatgg catccagtct ctgtttcaga gtctcaatat
    16801 agaggtacaa gacgtatcgc ctgatttgct acccgtcatg actgtgattc cgccaacatc
    16861 aacggtaaca cgcggcattc gggatatttc gtatgtcaaa ggtaaccgtt accacttttc
    16921 gcgcctggtt tttttagttt cacgacgaaa aaatggtcta aaacgtgatc aatttaacac
    16981 cttgctgatt gaccgtaaag aaagatgcgc tacatacaag tgtagcaccg tttattctct
    17041 gtaaattcct tattacgacg gcgtgaaacg cctgtcagga tccactgcca gacctcattt
    17101 tacgatttgc gcaggcgtct acgtttcacc acaacactga catcactctg gcaaggatgt
    17161 taggatggac cacggatgat aatgacggta acaagcatgt tagacaatgt tttaagaatt
    17221 gccacacgcc aaagcccact tgcactctgg caggcacact atgtcaaaga caagttgatg
    17281 gcgagccatc cgggcctggt ggttgaactg gtaccgatgg tgacgcgcgg cgatgtgatt
    17341 cttgatacgc cgctggcgaa agtaggcgga aaaggcttat ttgtaaaaga gctggaagtc
    17401 gcgctcctgg agaatcgcgc cgatatcgcc gtacattcaa tgaaagatgt gccggttgaa
    17461 ttcccgcaag gtctggggtt ggtcactatt tgtgagcgtg aagatcctcg cgatgccttt
    17521 gtgtccaata attatgacag tctggatgcg ttaccggcag gcagtatcgt cgggacgtcc
    17581 agtttacgtc gccagtgcca actggctgaa cgccgcccgg atctgattat ccgctccctg
    17641 cgcggcaacg tcggcactcg cctgagcaaa ctagataacg gcgaatacga tgccatcatt
    17701 cttgccgtag ccggactaaa acgtttaggt ctggagtccc gcattcgcgc cgcgttacca
    17761 cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg gtattgaatg ccgccttgat
    17821 gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc acgaaactgc actgcgcgtt
    17881 accgcagaac gcgccatgaa tacccgtctc gaaggtggat gtcaggtgcc aattggtagc
    17941 tacgccgagc ttattgatgg tgaaatctgg ctgcgtgcgc tggtcggcgc gccggacggt
    18001 tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa atgccgaaca aatggggatt
    18061 tcgctggcag aagagctact gaataacggc gcgcgcgaga tcctcgctga agtctataac
    18121 ggagacgccc cggcatgagt atcctggtca cccgcccgtc tcccgctgga gaagagttag
    18181 tgagccgtct gcgcacactg gggcaggtgg cctggcattt tccgctgatt gagttttctc
    18241 cgggtcgaca attaccgcaa cttgctgatc aactggcggc gctgggggag agcgatctgt
    18301 tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc acagctgcat cagcaagatc
    18361 gtaaatggcc ccgactacct gattatttcg ccattggacg caccaccgca ctggcgctac
    18421 ataccgtaag cggacagaag attctctacc cgcaggatcg ggaaatcagc gaagtcttgc
    18481 tacaattacc tgaattacaa aatattgcgg gcaaacgtgc gctgatatta cgtggcaatg
    18541 gcggtcgtga gctaattggg gataccctga cagcgcgcgg tgctgaggtc actttttgtg
    18601 aatgttatca acgatgcgca atccattacg atggtgcaga agaagcgatg cgctggcaat
    18661 cccgcgaggt gacgatggtc gttgttacca gcggtgaaat gttgcagcaa ctctggtcgc
    18721 tgatcccaca atggtatcgt gagcactggt tactacactg tcgactattg gtcgtcagtg
    18781 agcgtttggc gaaactcgcc cgggaactgg gctggcaaga cattaaggtc gccgataacg
    18841 ctgacaacga tgcgctttta cgggcattac aataactctc ataacaggaa gccataatga
    18901 cggaacaaga aaaaacctcc gccgtggttg aagagaccag ggaggccgtg gacaccacgt
    18961 cacaacctgt cgcaacagaa aaaaagagta agaacaatac cgcattgatt ctcagcgcgg
    19021 tggctatcgc tattgctctg gcggcgggcg tcggtttgta tggctggggt aaacaacagg
    19081 ccgtcaatca gaccgccacc agcgatgccc tggctaacca actgacggca ttgcaaaaag
    19141 cccaggagag ccaaaaagcc gagctggaag gcattattaa gcaacaagct gcacagctta
    19201 agcaggcgaa tcgtcagcaa gaaacgctgg caaaacagtt ggatgaagtc caacaaaagg
    19261 tcgccaccat ttccggcagc gatgctaaaa cctggctgct ggctcaggcc gattttctgg
    19321 tgaaactcgc cggacggaag ctgtggagcg atcaggacgt cacgaccgct gcagcgttgc
    19381 tgaaaagtgc agatgccagc ctggcggata tgaatgaccc gagtctgatt accgttcgtc
    19441 gggcaattac cgatgatatc gccagccttt ctgcagtttc gcaggtggat tatgacggca
    19501 tcatccttaa gcttaatcag ctttcaaatc aggtagataa cctgcgtctg gccgataatg
    19561 acagcgatgg ttcgccgatg gattccgacg gtgaagagct ttccagttcc atcagcgaat
    19621 ggcgtatcaa tctgcaaaaa agctggcaga actttatgga caacttcatt acgattcgcc
    19681 gtcgtgatga caccgccgta ccgctgttag cgccaaatca ggatatctat ctgcgcgaaa
    19741 atattcgctc tcgcctgctg gtcgcagcac aagctgtacc gcgtcatcag gaagagactt
    19801 atcgccaggc gctggagaac gtctccacct gggtacgtgc ttactacgat actgatgatg
    19861 ccaccaccaa agcattcctc gacgaggtgg accagttaag ccagcaaaat atctcgatgg
    19921 atcttccgga aaccctgcaa agccaggcga tgctggaaaa actgatgcag actcgcgtgc
    19981 gtaacctgct ggcacaaccg gcagcgggga caacggaagc taaacctgca cctgcacctg
    20041 cacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg
    20101 cacctgcacc tgcaccgcaa ggtgatactc cggcagccgc gccgcaagga gaataaccat
    20161 gctaaaagtg ttattactct ttgttttgct gattgcgggg atagtggttg gcccgatgat
    20221 tgccggtcat cagggttacg tgctgatcca gactgacaac tacaatatcg aaaccagcgt
    20281 cacgggcctg gcgattattt tgattctggc gatggtagtg ctgtttgcca ttgagtggct
    20341 actgcggcgg atcttccgca caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg
    20401 tcgccgtgcc cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag gcgattatca
    20461 gcaagttgaa aagctgatgg cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta
    20521 tctactggct gccgaagccg cgcaacaacg tggtgatgaa gcacgcgcca accaacatct
    20581 ggaacgcgca gcagagctgg ccggcaatga caccattccg gtagaaatca cccgtgtacg
    20641 tctgcaactg gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata agctgctgga
    20701 agttacgcca cgccatccgg aagtattacg tctggcggaa caggcgtata tccgcacagg
    20761 tgcatggagt tctctgctgg atatcatccc atcaatggcg aaagcccatg ttggtgatga
    20821 agaacatcgt gcaatgctgg aacaacaggc atggattggc ctgatggatc aggcgcgtgc
    20881 cgataacggt agtgaaggtt tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca
    20941 tcaggtagcg ttgcaggtgg caatggcgga acatcttatt gaatgtgacg atcatgatac
    21001 tgcccagcaa attatcatcg atggcctgaa acgccagtac gacgatcgcc tactgctgcc
    21061 gattcctcga ctgaaaacca acaatccgga acagctggaa aaagtgctgc gccagcagat
    21121 caaaaacgtc ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac tgatgaagca
    21181 cggagaatgg caggaagcat cgctcgcctt ccgcgcggcg ctgaaacaac gtccggacgc
    21241 ctacgattac gcatggcttg ccgacgcgct ggacagactg cacaagccgg aagaagctgc
    21301 agcgatgcgt cgcgacggtt tgatgttaac gttgcagaac aacccaccac agtagttcct
    21361 tctcacccgg aggcaagcac ctccgggacc tttctgatgc ataaaaaaac gcctgctctt
    21421 attacggagc aggcgttaaa acaggtctgt atgacaacaa gtgggtgctt cactcaacgt
    21481 tgtgtccatg gtgtctgatg aggcataagc gacatctgtc agtggacgat aagcaccgta
    21541 aacggctctg cgtcattccg gagtttatga ggcactaagg cgaacataag agatggaatg
    21601 agcatctacc cgtttattat gccacagaga atcgggaaat aacatccctt aacacttgtt
    21661 atgagataat tctgtaatcc tctttgcttc ctgagtaata acttcctgag tgaatattta
    21721 acctgagctt gatcctacac atacttatta tgaatgataa aattcattca attaataaca
    21781 catatattaa ttgccgttaa aactaaaaaa agcatcaata atcaacgcga tataataaac
    21841 ctgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat
    21901 tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt
    21961 caaggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac
    22021 cggagcattt tgcatggaat tttcgttttc acccaaactt tttgttgttg ctgtcgccgc
    22081 cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg
    22141 ctttgccgga tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg
    22201 cacgatcatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc
    22261 cacaaaatgg ccgctttttt acttattcac caccagcaaa tgtgcctgca taatgtcact
    22321 ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg
    22381 gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag
    22441 catgaagcgg tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca
    22501 ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat
    22561 catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg
    22621 atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg
    22681 atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat
    22741 cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc
    22801 accaaattca tcggggcgca ccgaccattc cttcagttcc ccctgaatat caccaggcgc
    22861 atgtagtttc agtccatcgc tggcagctgt ttcatcaccc aggcgctcga ccaccggaat
    22921 aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg
    22981 cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag
    23041 cgtcagggca cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg
    23101 attgtggatc tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag
    23161 aaatgcacac catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt
    23221 cctgccagca ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag
    23281 taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc
    23341 gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc
    23401 gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga
    23461 accactcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac
    23521 tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag
    23581 ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc
    23641 gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc
    23701 ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta
    23761 gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
    23821 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc
    23881 taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct
    23941 ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac taccaccgca
    24001 gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc
    24061 cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat
    24121 aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc gtttggttac
    24181 ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc tttcccgtgg cgattaaggc
    24241 caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccaaca aacagcgaca
    24301 tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta
    24361 cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat
    24421 gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa
    24481 gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca
    24541 ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaacg ggtacgccgt
    24601 gacgggaaac tttcgccatc gccgccggta actgacggtt tttcgccagt gcgtagagca
    24661 tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa
    24721 agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc
    24781 tgccgttgct gcctatttca ttccacggga agatggtgac gataacgaaa atcgcgccta
    24841 cgtagaaaat caggatccgc cacagcacct tgcctacggc actgcgcagc gtcacctgcg
    24901 gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca
    24961 caatacacag cgcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg
    25021 tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca
    25081 ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga
    25141 tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc actgcgatca
    25201 atgcgggtat ccactgcgcc atctccggga accagaactg gacataaacg ccaatggcgg
    25261 tgatttctga gatccccacc gccatccaca taaaccaata agaccaggcg gtgagataac
    25321 caaagaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta accggttcga
    25381 ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac gaatagcccg gcgatgatat
    25441 aggccaacaa tacggatggc ccggcccatt tcaaggtact ggcggccccc ataaacaggc
    25501 cgacgccaat ggtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct
    25561 gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt
    25621 taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat
    25681 ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg
    25741 agtgaccact ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag
    25801 cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg
    25861 tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg
    25921 aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag
    25981 tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg
    26041 ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga
    26101 ctgccaacga tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact
    26161 tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc
    26221 tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt
    26281 actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc
    26341 ctgacctcgg cgttatcttc aatggtcagc attttttcgg cattaatggc aaccagcgtt
    26401 ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg cgtgctgcat atcacgccaa
    26461 ccaatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt
    26521 atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt
    26581 caaaaagcca gtacaacagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa
    26641 agactacgcg tgagacaaac gaatccagcc cttcacgtgc cagcacgatc atattgaaga
    26701 tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattagtc tcgcggttgc
    26761 ccagctcata cagccagtcg aaccatttga tgatcagtcc aaccacaatc gccccgagtg
    26821 ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca
    26881 gtccggagtg gttattcagc acttcccagg taaagtagtt ggctgagttc agcaccatac
    26941 tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag
    27001 ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccatg
    27061 gcgagaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat
    27121 tcattccata gcgttttagc gccagccaga acatgcccac aatccccagc acgcccgccg
    27181 ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga
    27241 atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg
    27301 cgaccgtgct gacgaggaaa aacagccacg ctttgctatc ctggcgcaga aagtagacca
    27361 ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac
    27421 tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga
    27481 agatgccgac gcttaccagc gcgatgccca tcaggatcac ccacgtaaga ttggtctcca
    27541 cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg
    27601 ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca
    27661 agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga
    27721 agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca
    27781 cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacaa
    27841 caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc
    27901 ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt tatccaccga
    27961 cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag
    28021 caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt taagcacaca
    28081 aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat
    28141 aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc
    28201 gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc
    28261 aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc
    28321 aaattgctga tgaacggcgc acaaggcagc aatatggtca ttgctgcggt caccggagtt
    28381 ccccaccaga atggtcattt tcccttcacg ttgccgatcg ttcgccatcg tattgagaga
    28441 agggtccatc cgcgtcggga agtacagcag ttcgccccgc acctttggat gcgttttggc
    28501 aaaaaagctc aaatcgccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg
    28561 acgtagtggg taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc
    28621 ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag
    28681 tgtgggattg aactgaccgt ggaagaaaaa acgctgctga cggttagctt ttgctttcgc
    28741 gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca
    28801 gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt
    28861 cgcagccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga
    28921 tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa
    28981 caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca
    29041 tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct gatttccgcc
    29101 agaatataaa accgcaatga cgctttggcg atcaccagat aaccaaagac ataagcgccc
    29161 acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta
    29221 ttcgacaaca gcagccagat agcaaaatca cgcagcagcc agacggtaaa actcgccgcc
    29281 gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt
    29341 tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc
    29401 aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac
    29461 tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc
    29521 aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc
    29581 agatagctta acgggatgac accacgtttg atcaacatga tagcggcagg aattaccacc
    29641 agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg
    29701 acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg
    29761 gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc
    29821 accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacaa tccctggctg
    29881 attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc
    29941 gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg
    30001 tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac ggtaatcaac
    30061 tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc cagcaactta
    30121 ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct
    30181 tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt
    30241 gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt
    30301 gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg
    30361 aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg
    30421 gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg
    30481 aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat cgtagtagtt
    30541 ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag
    30601 gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt
    30661 atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc
    30721 tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata
    30781 gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc
    30841 tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat tatgcttttt
    30901 cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat
    30961 aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc
    31021 cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat tggcggtgga
    31081 gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc
    31141 cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg
    31201 ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct
    31261 acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg
    31321 aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta
    31381 tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac
    31441 cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctaca ccgcgtccag
    31501 ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca taaccgcgaa
    31561 taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg cgcacggcat
    31621 tttcaatcca ctgtgcataa aagcgtctgc tggcgtcagg cgcataccac ggcgcacgaa
    31681 aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata tcggtctctt
    31741 gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa tcaacttcac
    31801 cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta tttgatgccg
    31861 caattttggc ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt
    31921 cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg
    31981 gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt
    32041 ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag
    32101 gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaag
    32161 gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg
    32221 agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc
    32281 agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag
    32341 aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttccagcgag
    32401 atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc catcacctga
    32461 tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag
    32521 ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc gttgaggaag
    32581 gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc
    32641 agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc
    32701 ggcgtagtga tgatgagaat ttcgcggata ccggccaaca tcagcaccga cagcggatag
    32761 taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga
    32821 tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc ctccgctgaa
    32881 aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac ctgcttccac
    32941 cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc aaaggtttcc
    33001 tgtggcagcc aaccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca
    33061 tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcgccac gccgtgcggc
    33121 ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta
    33181 cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa
    33241 tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca
    33301 tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc
    33361 ggaaagtggt aaggaccgta gttattcgag cagttggtga tcagcgtcgg cagaccgtag
    33421 gtccgcagcc aggcgcgcac cagatggtcg ctgccggctt ttgacgcgga ataagggctg
    33481 ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat
    33541 acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta
    33601 ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata
    33661 aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca
    33721 cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact
    33781 ttctcaaagg caaagtgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg
    33841 taggtcagct tatcgaccac taccaccgca tcgctcgttt cgttgatgat ataacgcacc
    33901 agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga
    33961 ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata actttgaact
    34021 gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg
    34081 ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca
    34141 gcgtttcgcc gctgtgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc
    34201 gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg
    34261 cgcgtttatc ggtagcagca aggcagtcgg caaccgccgc tttcacctga tcgataaccc
    34321 agaacggttt gtgatcgtta acttcgcgcg cggtacggat aagccgcgcc tgctgggggt
    34381 tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag
    34441 gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa
    34501 tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga
    34561 agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac
    34621 cttcgaggaa aattttgtac agttcgctgg cgcgggtcga acaaaccggc gtcataccac
    34681 caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac acgcgttccg
    34741 ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg
    34801 gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt
    34861 caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa
    34921 cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca atcagccagg
    34981 catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta
    35041 ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg
    35101 cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg
    35161 taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtcatat
    35221 ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt
    35281 atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc
    35341 gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg
    35401 acgctcagtg gtatcgcgca tcactagcac cggtttcccc agcgaaggcg cttcttcctg
    35461 aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa
    35521 atactcctgg ggattgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg
    35581 ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt
    35641 ggcgatgtct gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct
    35701 gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat ttgccgccag
    35761 ttctgaacgc agcgtgtcgc tgctcatcac ctgatcacgc acccataaca gtgcatcaat
    35821 gactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg
    35881 ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt
    35941 agcctcttcc ggccacggcg aatagagatc gcccgtgcgt aaaccagcct caacgtgacc
    36001 aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc
    36061 gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat
    36121 ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg
    36181 tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg cagtgacgca
    36241 aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggcg ccatcttgat
    36301 ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc
    36361 ggcgagcgcc tttgcgctcg cagcagcagt gttgctattt cgagcaacgg cgggttaatg
    36421 cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac
    36481 gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac
    36541 gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc tgatcatagt
    36601 cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac
    36661 gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc
    36721 gcgaaatatt atgctgctca gcaattttca gcgcctgctc aatgctgttc atccggcggt
    36781 cgtagatggc tttcgccacc tcttcctgac gcttcacctg agctttcatc tggatggtgc
    36841 gtgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa
    36901 aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcggca ataagcttca
    36961 cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg ttaatcattt
    37021 catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg taatagtcgg
    37081 tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat
    37141 aggcttcgtc catgaccgat ggttgatcgg cagaagccat gtttgaacgg acatccaggt
    37201 tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg
    37261 taatcgccgt cgagctccac tcctgacgag caaaaaaagt atacgccagc gcgattaacg
    37321 caaacgccag ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc
    37381 cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca
    37441 tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg
    37501 ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag
    37561 gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc
    37621 tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg
    37681 acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa
    37741 tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat
    37801 ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc
    37861 gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac
    37921 tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat
    37981 catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat
    38041 tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt
    38101 gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag
    38161 caccatctcc caggagccaa agatataacc caggctactg agataaagtt tgccgaacac
    38221 catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa
    38281 acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc
    38341 atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat
    38401 ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa
    38461 accgactttt tttgccactt tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat
    38521 actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta
    38581 ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac
    38641 cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacatgcc agcaaattcc
    38701 aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa gcaaaacgcc
    38761 acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag
    38821 aaatcgtcat tggtcttagt catggccagt ttattaatga ggaattccat tgcatcgatt
    38881 tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagtc
    38941 gtgagcagct cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt
    39001 ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg
    39061 taaataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatggtcagg
    39121 ctgccgccct cttccacgtt acgcgccgca ccgaagaagc gtttcggacg atgaagggca
    39181 ttggcgtcca caccaccggt caacacttta cctgacgccg gaacaacagt gttgtaagcg
    39241 cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg
    39301 cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca
    39361 aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga
    39421 cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctga
    39481 gcaatgttct gcagcagcat agttttaccg gctttcggcg gtgccacaat cagaccacgc
    39541 tgaccacgac cgataggtga tgccagatcc agtacgcgag cagttaaatc ttcagtagaa
    39601 ccgttaccac gttccatacg cagacgagag tttgcgtgca gcggggttaa gttctcaaag
    39661 aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc
    39721 gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg
    39781 cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg
    39841 taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca
    39901 tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc
    39961 tgcttacgca tacgagccag gttttccagc cccatatttt cgccgagagt gatcagctca
    40021 gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg
    40081 gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt
    40141 catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa
    40201 gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc
    40261 gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt
    40321 tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc
    40381 cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt gcacccactt
    40441 tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat
    40501 atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct
    40561 gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact
    40621 ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt
    40681 cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt
    40741 gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata
    40801 cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa
    40861 cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa
    40921 aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc
    40981 gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa
    41041 cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccacgtg
    41101 ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc
    41161 tggcggaagc taccggcctg aagctgggtc tggcttacgg tggtgatggc tacgacaaac
    41221 agctgaaagt gctggaaagc ggcgttgaca ttctgattgg cactacgggg cgtttaattg
    41281 actacgccaa gcagaaccac attaacctcg gtgccattca ggtggtggta ctggacgaag
    41341 ccgatcgcat gtacgatctg ggctttatta aagatatccg ctggctgttc cgccgtatgc
    41401 cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg
    41461 aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa
    41521 cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc
    41581 tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaac
    41641 accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga
    41701 caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc
    41761 tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc
    41821 acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta
    41881 caggtcgcgc aggcgcaagc ggtcactcta ttagcctggc gtgtgaagag tatgcattga
    41941 atttgcctgc tattgagacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg
    42001 acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg
    42061 gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata
    42121 ctcaaaatca ttcaaactgt atcaagatgg caagagaatg agtcccgatg agcgtactgt
    42181 agtaagtgat tcgggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt
    42241 atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg
    42301 ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaattaa acgcaaagtg
    42361 cgtctggctg ctggcctgaa tagcgaaaat gccctctcta atgaagcaat ggagcgcggt
    42421 tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata ttcctccctc gcaaattcgc
    42481 gttgtcgcta cggcgacatt acgacttgcc gtcaatgcgg gtgattttat tgccaaagcg
    42541 caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc acgtctgatt
    42601 tatcagggcg ttgctcacac cactggtggt gccgatcagc gcctggtggt ggatataggc
    42661 ggagccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg
    42721 tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaggaa
    42781 aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta
    42841 cggtatcacg gctggaaagt gtgcgttggc gcttccggca ccgtgcaggc gttacaggaa
    42901 atcatgatgg cacagggaat ggatgaacgc attaccctgg aaaagttgca gcaactgaaa
    42961 cagcgcgcca ttcattgcgg tcggctggaa gagctggaga ttgacgggct gacgctggaa
    43021 cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat
    43081 attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg
    43141 catctggccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca gcgccgcttt
    43201 atgatcgata ttgatcaggc gcagcgcgta gccaaagttg cagctaactt cttcgatcag
    43261 gtggaaaatg aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcttgtcag
    43321 cttcatgaaa tcggcctgag cgttgacttc aaacaagcgc cgcaacacgc tgcttatctg
    43381 gtgcgtaacc tggatcttcc cggttttacc cccgcacaga agaaactgct ggcgacgcta
    43441 ctgctcaacc agactaaccc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtacca
    43501 ccgcgcgtcg cagaacaact ctgccgttta ctacgcctgg ctatcatttt tgccagccgt
    43561 cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg
    43621 acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa
    43681 agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga
    43741 tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc gccatcatcg
    43801 ctttcaaatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg ctgaccactt
    43861 tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct
    43921 ccgggcgcac cagttcgcca tactgacggc gttctttaca cagcgtaaag gtcaattcct
    43981 tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat
    44041 cgatgctgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa
    44101 actccagccc tttcgacgcg tggagagtca tcagttgcac ctgatccagc tcttcttcac
    44161 tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca
    44221 tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga
    44281 cgttcttcat gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac agccaggatt
    44341 cataatccat gccgtggatc agatcgcgca ccgcagcaat cggctcccgc tccgccaggc
    44401 gctggatttc tgccaaccag tgagtgaagc gggtcaatgc ttcataacca cgtccgctaa
    44461 gggtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg
    44521 cccactcacc cagctttttc agcgtcgccg ggccaatctc tcgcttcggc gtgttaacga
    44581 tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac acgcagataa gccagcaaat
    44641 ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt
    44701 tttgcatcag gaatttttca aatacccgcg actgatggtt gccgcgataa agaatggcgt
    44761 aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcaccg gtgacgcgct
    44821 cggcttcatg ttcctcgtta ttcgcgctta aaacttttag ctctgtgcca taacccagtt
    44881 cggagaacag acgcttttca aagacgtgcg gattattggc gatcaggatg ttcgccgctt
    44941 tcaaaatacg tccggaagag cggtagtttt gctcaagctt aatcaccttc agcgccggaa
    45001 aatcctggct caacagcacc aggttttgcg gacgtgcgcc gcgccaggag tagatcgact
    45061 ggtcgtcatc gccaactacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt
    45121 catactggct ggtgttggtg tcctgatact catccaccag caggtagcga attttgttct
    45181 gccagcgctc gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat
    45241 catcgaagtc gagaacgtta caggctttca ggtgtgcatc atacagccca taacaatggg
    45301 caaaaatacg atcccgctcg ccctttgcct ctgccgccgc ctgcgccgga gttttgagat
    45361 cgttcttcca gttggagatg gtcgaaatta gctgttgcag gagaactttg tcatcttcaa
    45421 tcagcccctc agtcagctct ttgagcaacg ccagctgatc ggtatcgtca aacaatgaga
    45481 agttcgcttt catcccaagc gccgcatact cacgcttgat gatatccagc cccagcgtgt
    45541 ggaaggtgga gatcatcagc cctcgcgcct ctttgcgacc cagcgtctgc ccgactcgct
    45601 ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgtgcctgat
    45661 aaccgcaacc gcggatcaga tgggcgattt tattggtgat aacacgagtt ttaccggaac
    45721 ccgcgcccgc cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg
    45781 ggtttagacg cataggtgta ttgctcaatc ttcgaacggg gggaggattg tagcagaatc
    45841 gcatttgaat gtgaccgagt gaaaaccgct aaccggatgg ctgcggatgt ttaaatcaga
    45901 gagggaaggc cgaaaccgga gttcatcatg aatccattag acttagaaaa tatcgggtcg
    45961 taacgttgtg ttattacttt ttcagagttt actaaggaga ggtttactcg tggcgattaa
    46021 tttcagcccc aaggttggtg aaatactgga atgcaacttt ggtaactatc ctgttagcca
    46081 gaatggacag atcagtacgg cgtattacga tggccgcatt ccacctgaga tgataaaaaa
    46141 taggttggtt gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt
    46201 atcaaccact cgcgaccatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa
    46261 tgttatcaat gacctgcaat tctttgatca gcagatacgt tgggctaaag ccgacctggt
    46321 acaacaagtc agtcgaaacc gattaaacag agcaaggact tatcgggggt atcttaatca
    46381 atgtttgcca catgagttgg ttgcagacat acagagggca gtcatcaagt caatcaatgc
    46441 catatcgctc ataaattgac aataaaatca ccatccccga taatgactct gtagcccata
    46501 gaggccctaa gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc
    46561 catatatgtc cccccctccc tgactgacaa tttttaattc aaggcacgat catggcaaaa
    46621 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga acttctcgag
    46681 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    46741 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    46801 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    46861 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaagggc
    46921 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    46981 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    47041 cagttgcgcg ttatctaccg cgccttccgg aatagcttta cccaagtcgc ccggttgcag
    47101 ctctgccata aacggtttca gcaacggcac acaagcgtaa gagaacagat agttaccgta
    47161 ctcagcggta tcagagataa ccacgttcat ttcgtacaga cgcttacggg cgatggtgtt
    47221 ggcaatcagc ggcagctcgt gcagtgattc ataatatgca gactcttcaa tgatgccgga
    47281 atcgaccatg gtttcgaacg ccagttcaac gcccgctttt accatcgcga tcatcagtac
    47341 gcctttatcg aagtactcct gctcgccgat tttaccttca tactgcggcg cggtttcaaa
    47401 cgcggttttg ccggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    47461 cgccatcata ccggaagaga attcgccgga gatgatgtcg tccatatgtt tctggaacag
    47521 cggtgccatg atctctttca gctgttctga cagcgcataa gcacgcagtt tcgccgggtt
    47581 agagagacgg tccatcatca gggtgatgcc gccctgcttc agcgcttcgg tgatggtttc
    47641 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    47701 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    47761 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    47821 ggttgctgcc gcccatgctt tagcgatcgc cataccttcg cctttcggat cgttttccgg
    47881 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    47941 gccagggcat ttcggcgcaa ccatgactac ggtgatatct ttacggatct gctcgcccac
    48001 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    48061 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    48121 ctgcgggatc agttcttcgt aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca
    48181 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat ccagaccaga
    48241 atcacgcatg ttcaggccct ggttcagacc ctgtgcgcca cagccgacga tgactacttt
    48301 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    48361 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    48421 tcctcgtgat gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata
    48481 tgacaggaaa tttattgcgg aaattgatat attcacaacg tcacattgca atttttgcaa
    48541 cgtcaacatc gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg
    48601 cggaaagccg ccattttggc cgcagcgcgc gggcgatgca cgttagccca tccacgctct
    48661 cacggcagat tcagcgcctg gaagaagatc ttggtcagcc gctatttgtg cgcgataacc
    48721 gcacggtaac gctgacggaa gcgggcgaag agctgcgcgt tttcgcccag caaacgctgt
    48781 tgcagtatca gcaattgcgc cacaccatcg atcagcaagg gccgtcgctc tctggcgaat
    48841 tacatatctt ctgctcggtg accgctgcct acagccatct gccgccgatt ctggatcgct
    48901 tccgcgcgga acacccgtcg gtggagatca aactcaccac tggtgatgcg gcagatgcaa
    48961 tggaaaaagt ggtcacggga gaagcggatc tggcgattgc cgggaaaccg gagacgctac
    49021 ccggcgctgt ggcgttttcg atgctggaga atctggcagt agtgctgatt gcgcccgcgc
    49081 tgccctgccc ggtgcgtaac caggtttcgg cagagaagcc ggactggtct acagtgccgt
    49141 ttatcatggc cgatcaggga ccggtgcgtc gccgcattga actgtggttt cgacgcaata
    49201 aaatcagcaa cccgatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg
    49261 tggcgctcgg ctgtggtgtg gcgctgttgc cggaagtagt gctggaaaac agccccgaac
    49321 cggtgcgtaa ccgcgtgatg attttagagc gcagcgatga aaaaacgccg tttgaactgg
    49381 gcgtgtgtgc acaaaaaaag cggctgcatg agccgctgat tgaggcgttc tggaggattt
    49441 tgccgaacca caaatgacgt tgtcgcgcgg gtaggcctga taagcgaagc gctatcaggc
    49501 atttttccct aacccgccaa aaagaacctg aacgccgggt tattggtttc gtcgtggcaa
    49561 tcgtagccca gctcattcag ccgggtttcg aaatccggtt catggtcgcc aagttcgaac
    49621 gccgccagta cgcgcccgta gtcggtgcca tggctgcgat agtggaacaa agaaatgttc
    49681 cagtgcgtac ccagcgtgtt gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg
    49741 aagctgtaga ggcgttcctg caacggatgt gacggacgcc cgccgaccat atagcgcaca
    49801 tgcaacttcg ccatttcgtc gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc
    49861 atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg
    49921 caggcgtttt tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc
    49981 agttggcaga atttgaggaa gctgcctttt tcttccggaa tggtcaccgc caacaacgct
    50041 tcacgctgtt cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg
    50101 ttggcaccgg aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat
    50161 tttttcattc ccgccagcgc cagcgcgcca gagggttccg ccaccgcgcg cacatcttcg
    50221 aataaatcct tcatcgccgc acagatcgca tcactatcga cggtgatgat gtcgtcgaga
    50281 tactcctggc ataaacggaa ggtttcgtca ccgatgcgtt ttaccgctac gccttcagca
    50341 aatagcccta cgcgcggcag atcaaccgga tgacccgcat ccagcgctgc tttcaggcag
    50401 gcggagtctt ccgcttctac ggcgatcact ttgatttgtg gcatcagttg tttgatcagc
    50461 accgcaacgc ccgccgccag accgccgccg ccgactggca caaatacgcg gtcgagatgg
    50521 gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc cggcaatcac catcggatgg
    50581 tcgaacggcg gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc tttagctttc
    50641 gcttcgtcaa agttcgcgcc atgaagcagc acttcgccgc cgaatccacg taccgcatcg
    50701 actttgatgt cggcggtggc ggttggcata acgatcaggg ccttcacgcc taaccgcgca
    50761 gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct
    50821 ttttgttctt ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc
    50881 actggctggc gatcttcgcg cttcaccaga atgacgttat caagacgcga cgacagtttt
    50941 tccatttttt gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct
    51001 cttaaatatt cggcaccttc cggagcaccg gacaggggtt gcgagtcagc cattattaac
    51061 cccccagttt cgatttatcg cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt
    51121 aggcgcgcag ggcaaaggaa acctgacgtt cacggttttt cggcgtccag gctttgtcac
    51181 cccgagcttc ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa
    51241 tgccacggtt cgggatgtcg atagcgatca ggtcaccatc ttcaatcaga ccaatgctgc
    51301 cgccgcttgc cgcttccggt gagacgtggc cgatggaaag accagaggtg ccgccagaga
    51361 aacgaccgtc ggtgatcagc gcacaggctt taccgagacc cattgatttc aggaagctgg
    51421 ttgggtagag catttcctgc atgccagggc cgcctttcgg gccttcatag cggatcacca
    51481 ccacatcgcc cgcgacaact ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt
    51541 cgtacacttt cgccgggccg gtgaatttga ggatgctgtc atcgacgccc gcggttttaa
    51601 cgatgcagcc gttttctgcg aaattaccgt agagcaccgc caggccgccg tctttgctgt
    51661 aggcgtgttc cagcgagcgg atacagccat tggcgcgatc gtcatcgaga gaatcccaac
    51721 ggcaatcctg cgagaatgcc tgtgtggtac gaatgcctgc aggacctgcg cggaacatat
    51781 tttttaccgc gtcatcctgg gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg
    51841 tcaggccaag tacgtttttc acatcacggt tcagtaaccc cgcgcgatcc agttcgccga
    51901 gaataccgat aacaccacca gcacggtgaa catcttccat atggtatttc tgggtgctcg
    51961 gcgcaacttt acacagctgt ggaaccttgc gggaaagctt atcgatatca ctcatggtga
    52021 agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag tacggtgtta gtcgatccac
    52081 ccatcgcgat atccagcgtc atggcgtttt caaacgccgc tttgctagca atattgcgcg
    52141 gcagtgcact ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca atgcgtttac
    52201 cggcattgag gaacaactgc ttacggtcgg cgtgggttgc cagcagcgag ccgttgcccg
    52261 gctgcgacag acccagcgct tcggtcaggc agttcattga gttagcggta aacatcccgg
    52321 agcaggagcc gcaggtcgga cacgcggaac gttcaacctg atcgctctgg gagtcagaaa
    52381 ctttcgggtc tgcgccctgg atcatcgcat caaccagatc gagtttgatt atctgatcgg
    52441 acagtttggt tttcccggcc tccatcgggc cgccggaaac aaagatcacc ggaatattca
    52501 ggcgcaggga agccatcagc atccccgggg tgattttgtc gcagttagag atgcagacca
    52561 tggcgtcggc gcagtgggcg ttgaccatat actcaacgga atcagcgatc agttcgcgag
    52621 atggcagtga ataaagcatc cccccgtggc ccatggcaat cccatcatcc accgcaatgg
    52681 tgttgaactc tttggcaacg ccgccagccg cttcaatttg ttcggcgacc agtttaccga
    52741 gatcgcgcag atggacgtga cccggtacaa attgggtgaa cgagttcaca accgcgataa
    52801 tcggcttgcc aaaatcggcg tcggtcattc cggtggcgcg ccacagcgca cgagcacccg
    52861 ccatattacg accatgagtg gtggtggcgg aacggtactt aggcatactt tatttactcc
    52921 cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat ttattgatta
    52981 acttgatcta accagcccca tttatcttcg gtttcgccag tgaagaggcc gaagaaggct
    53041 tgctgaatgc gtttggtaac cgggccacaa cggccttcgc caacctgaat accgtctacg
    53101 ctgcgcactg gcgtgatttc tgccgccgta ccggacataa acacttcatc cgccaggtac
    53161 agggattcgc gcgacagcac ctgctcacgt acttcaattc ccagctcttt cgccagtttg
    53221 atgatggcat cacgggtaat acccggcagc gcggaggagg tgaacggtgg ggtgaacagc
    53281 acaccatctt tcacttcaaa caggttttcg cctgcgcctt cagagatata accgttcaca
    53341 tccagcgcga taccttcctg ataaccgtgg cggcgcgctt cgctacccac cagcagggaa
    53401 gagaggtagt taccaccggc ttttgccgcc gtcgggatgg tgtttggtgc tgcgcggttc
    53461 caggaggaaa ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag atacgctccc
    53521 cacgggaaag cagcgataat cacgtcggtt gagtatcccg ctggcgggtt tactcccatg
    53581 ccaacatcac caacgaagat cagcggacgg atataggcgc tggtgagatt atttttgcgg
    53641 atcacgtcac gacaagcttc catcagctca tcaatgctct gcgaaaccgg gaagcgatag
    53701 attttggcgg agtcatgcag acgctgcata tgctcacgat ggcggaatac aaccggtcct
    53761 ttgtgcgagt cgtagcaacg gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc
    53821 gacatcacat gcaccttcgc gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa
    53881 tcagctttct tcgtggtcat ttttatattc cttttgcgct caggcgcgga tttgttgtga
    53941 tgtggttgtg ctctggcaga tggcaacgtg tgcgacgtcc accagtttat ttaactgact
    54001 aaacagtaag tcgaccgacc gtgggctggc aacggtcaat tcgatattta tattttgtgc
    54061 atcgctggcg gcggccatat tcattgagca gacgtggaaa ccacgatgac gcaccacgcg
    54121 taaaacacgt tctaaggttt ccggattgaa gcgagccgat acattgacct gatgttgcat
    54181 catgataatt tctccaacat ttctgaatta ctggcaccag gcggcaccag cggccagacg
    54241 ttctcaagtt cgtcgattga gacatgaagc aggtatggcc catcactgtt cagcatggtg
    54301 tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg
    54361 ctggctaaca tgagaaaatc ggggttatcg gtaagggtgg tttcgctata tcgttcctgg
    54421 aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt ggttatcgag taagacgatt
    54481 ttcaacggta actgcttacg ttttacggtg cccagctctt gtacattcat catgaaagag
    54541 ccgtcaccgg agatacagac gacggtatcg ttcggtcgcg cgacttgcgc gccaaccgcc
    54601 gccggtaaac cgaaacccat ggtgcctaag ccgctggagg tgatgaaatt ttccgggcga
    54661 gtatgggcga tgtgctgcgc agcccacatc tggtgctgcc ccacatctgt ggtcacgacg
    54721 caatccgcag gtttacgatc cgacagttgt tttaacaaca atggcgcgta gatagcgtca
    54781 ccgggatggt cgtaacgcca ggcatgttca tcacgcagct gcgcgcagtg tagctgccag
    54841 tcattgatat ttaacggctg ctgtaatgct ggtaacagag catttaaatc accttgtaat
    54901 gccacatgtg cctgacgcag cttgttcatt tctgccgggt cgatatccat atggataaca
    54961 ctggcgtgtg gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc
    55021 acggcgatca gcaagtcgca ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt
    55081 cccagcatgc ccagatagta cggataatct gcttcaactg cgcccagccc tttcagcgtg
    55141 caggtggcag gcatttttgt ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc
    55201 atacccacgc caccaccaac gtacagcatc ggtttttgcg cttttgccag catctggcgc
    55261 gcttgctcga cttcggcatg tgggaaagtc acttcgtttt caacggtggt gaaccacggt
    55321 tccaggtcac cgctggctag ctggatatct tttgggatat cgaccagaac cggaccagga
    55381 cgacctgagc tggcaacgtc gaatgcttca gccatgatgc gcggcaattc ttccagcgac
    55441 tgcaccagaa agctgtgctt ggtacaggct aacgacaatc ccaggacatc cacttcctga
    55501 aatgcgtcag tgccgataaa cggtgcggac acttgaccgg tgatggcaac aacagggata
    55561 gaatctaaca gtgcgtccgc aagcccggtt atcaggttgg ttgcgcccgg accagacgtg
    55621 gcgatacata cgccagtttt gccggtagca cgggcataac cgatagccgc cattgccgca
    55681 ccctgctcat gtcggcacag caagtgctcc acgccgccgt catacaatgc atcgtaaacc
    55741 ggcataattg cgccacccgg ataaccgaaa acggtgttca caccctgtgc ccgcaacgca
    55801 tgtaccaccc actgtgcgcc attcatagtt agttccccgt cctgaatctt gagaaacaga
    55861 attttgtgct gttattcatt gtctgctcct cggttatgtt tttaaggtca aaaaaaaccc
    55921 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    55981 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    56041 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    56101 aaagagttac cataggtttt gtaaatagta caagatattt tttggccgat ttttatgatg
    56161 cgtttaataa tttcaatata aattgttgtt ttttaaaaat aaaatattga gtgaaaaaaa
    56221 ttcaatttca gtatgaatgt tgttcccttc ctcgtagttc atcctttgtc cgctgcattt
    56281 ttgcgagcat cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat
    56341 cagccactaa aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatggt
    56401 gactcaagga gggcttatgt cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa
    56461 tgcgcccccg atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt
    56521 gggcttacca gaaacaacgg taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa
    56581 tagcggatat gaatatccgg cgaaaaaaat caccatcaac ctggctccag ccgatctgcc
    56641 aaaagaaggg ggacgatatg atttacctat cgccattgcg ttgctggcgg cctcagaaca
    56701 gcttacagcc aataagttag atgaatatga attagtcgga gaactggctc ttacaggcgc
    56761 tctgcgtggc gttcccggtg caatctccag tgcaactgaa gctattaagt cgggcagaaa
    56821 aattatcgtc gcgaaagata acgaagatga agtggggcta attaacggtg aaggatgcct
    56881 gatagccgat catctgcaag ctgtctgtgc gtttctggaa ggtaagcacg ctctcgaacg
    56941 cccgaaacca actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg
    57001 tcaggaacaa ggaaagcgag gactggaaat taccgccgct ggcgggcaca accttttact
    57061 gattgggccg ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc
    57121 agatttaagc aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga
    57181 atcagtacaa aaacaatggc ggcagcgccc gttccgctca cctcatcaca gtgcgtcgtt
    57241 aactgcgatg gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt tggcgcataa
    57301 cggcgtgctt tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg
    57361 agagccgatt gaatccgggc agatccatct ttcacgcaca cgagcaaaaa taacctatcc
    57421 agcccgtttc cagcttgtcg cggcgatgaa tcccagccct accggacatt atcagggaaa
    57481 ccataaccgc tgcacgccag aacagacatt acgttatctc aaccggctct cggggccctt
    57541 tctcgaccgc ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa
    57601 aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga
    57661 gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca
    57721 attctgcaaa cttgagagcg aagatgcgat gtggttggaa ggaacactga tccatctggg
    57781 gttatcgatt cgtgcctggc agcggttatt gaaagttgca cgaaccattg ctgatattga
    57841 tcagtctgac attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga
    57901 ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt
    57961 attaatcatc agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa
    58021 gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg
    58081 cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg
    58141 taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca
    58201 gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat
    58261 tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt
    58321 tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca
    58381 tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa
    58441 ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga
    58501 cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt gtgaaccttt
    58561 tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg
    58621 cagaaacgct catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac
    58681 ataacgagtt ttctatcggt gccagcgcct cattgtggga atgtatgctt aatcagtggc
    58741 tgggacgttt gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg
    58801 cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca
    58861 ctgaagcgcc caaaatggac gaatttagta gtcagttgct gggatatttc actttagcgc
    58921 tttataccag tgccccttca aaactaaagg gagatcttaa ttatctgcga cttgagtggg
    58981 ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtg cccattctga
    59041 caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc
    59101 tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac
    59161 tttcacggcc gctttatgcc atatggctgc aaaatagcga taaaaatacg ttgattcgcg
    59221 atcttttgaa aattaacgtg ctggatgaag tgtattaata tgaatggctg gcaaggatgc
    59281 cggtagaagg atttacttcg gagagggtta tttcagataa aaaaaatcct tagctttcgc
    59341 taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac
    59401 cggtgctcta ccaattgaac tacgccccta attagggtgg cggaacggac gggactcgaa
    59461 cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa
    59521 ttcttttaca accaccggtt ttatgaccgg cttactgctt aatttgatgc ctggcagttc
    59581 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
    59641 ttcggcatgg ggtcaggtgg gaccaccgcg ctacggccgc caggcaaatt ctgttttatc
    59701 agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc tc
//
LOCUS       DAMZII010000012        59435 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZII010000012 DAMZII010000000
VERSION     DAMZII010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.012"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(86..994)
                     /locus_tag="POA77_000717"
     CDS             complement(86..994)
                     /locus_tag="POA77_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6364195.1"
                     /translation="MTFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            1170..2480
                     /gene="abgA"
                     /locus_tag="POA77_000718"
     CDS             1170..2480
                     /gene="abgA"
                     /locus_tag="POA77_000718"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDI6364196.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            2480..3925
                     /gene="abgB"
                     /locus_tag="POA77_000719"
     CDS             2480..3925
                     /gene="abgB"
                     /locus_tag="POA77_000719"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDI6364197.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            3962..5488
                     /gene="abgT"
                     /locus_tag="POA77_000720"
     CDS             3962..5488
                     /gene="abgT"
                     /locus_tag="POA77_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDI6364198.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            5499..6014
                     /gene="ogt"
                     /locus_tag="POA77_000721"
     CDS             5499..6014
                     /gene="ogt"
                     /locus_tag="POA77_000721"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI6364199.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            6209..6961
                     /gene="fnr"
                     /locus_tag="POA77_000722"
     CDS             6209..6961
                     /gene="fnr"
                     /locus_tag="POA77_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDI6364200.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            7113..8063
                     /gene="uspE"
                     /locus_tag="POA77_000723"
     CDS             7113..8063
                     /gene="uspE"
                     /locus_tag="POA77_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDI6364201.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKEMIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8113..8370)
                     /locus_tag="POA77_000724"
     CDS             complement(8113..8370)
                     /locus_tag="POA77_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDI6364202.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            8614..9645
                     /gene="ynaI"
                     /locus_tag="POA77_000725"
     CDS             8614..9645
                     /gene="ynaI"
                     /locus_tag="POA77_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDI6364203.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(9696..11309)
                     /gene="mppA"
                     /locus_tag="POA77_000726"
     CDS             complement(9696..11309)
                     /gene="mppA"
                     /locus_tag="POA77_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDI6364204.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(11646..12545)
                     /gene="pgrR"
                     /locus_tag="POA77_000727"
     CDS             complement(11646..12545)
                     /gene="pgrR"
                     /locus_tag="POA77_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDI6364205.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            12683..13603
                     /locus_tag="POA77_000728"
     CDS             12683..13603
                     /locus_tag="POA77_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6364206.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            13603..14535
                     /locus_tag="POA77_000729"
     CDS             13603..14535
                     /locus_tag="POA77_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6364207.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            14553..15191
                     /locus_tag="POA77_000730"
     CDS             14553..15191
                     /locus_tag="POA77_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6364208.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            15500..16228
                     /gene="mpaA"
                     /locus_tag="POA77_000731"
     CDS             15500..16228
                     /gene="mpaA"
                     /locus_tag="POA77_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDI6364209.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLHWHPKDAIRLS"
     gene            complement(16203..17168)
                     /gene="ycjG"
                     /locus_tag="POA77_000732"
     CDS             complement(16203..17168)
                     /gene="ycjG"
                     /locus_tag="POA77_000732"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDI6364210.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            17287..17793
                     /gene="tpx"
                     /locus_tag="POA77_000733"
     CDS             17287..17793
                     /gene="tpx"
                     /locus_tag="POA77_000733"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDI6364211.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(17837..19378)
                     /gene="tyrR"
                     /locus_tag="POA77_000734"
     CDS             complement(17837..19378)
                     /gene="tyrR"
                     /locus_tag="POA77_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDI6364212.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(19526..20587)
                     /locus_tag="POA77_000735"
     CDS             complement(19526..20587)
                     /locus_tag="POA77_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDI6364213.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(20584..21981)
                     /locus_tag="POA77_000736"
     CDS             complement(20584..21981)
                     /locus_tag="POA77_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDI6364214.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            22136..23134
                     /locus_tag="POA77_000737"
     CDS             22136..23134
                     /locus_tag="POA77_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6364215.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(23245..24150)
                     /gene="ompG"
                     /locus_tag="POA77_000738"
     CDS             complement(23245..24150)
                     /gene="ompG"
                     /locus_tag="POA77_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDI6364216.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(24195..25277)
                     /locus_tag="POA77_000739"
     CDS             complement(24195..25277)
                     /locus_tag="POA77_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6364217.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(25291..25950)
                     /gene="pgmB"
                     /locus_tag="POA77_000740"
     CDS             complement(25291..25950)
                     /gene="pgmB"
                     /locus_tag="POA77_000740"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDI6364218.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(25947..28214)
                     /gene="ycjT"
                     /locus_tag="POA77_000741"
     CDS             complement(25947..28214)
                     /gene="ycjT"
                     /locus_tag="POA77_000741"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDI6364219.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(28211..29266)
                     /locus_tag="POA77_000742"
     CDS             complement(28211..29266)
                     /locus_tag="POA77_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6364220.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(29276..30064)
                     /locus_tag="POA77_000743"
     CDS             complement(29276..30064)
                     /locus_tag="POA77_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6364221.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(30082..31134)
                     /locus_tag="POA77_000744"
     CDS             complement(30082..31134)
                     /locus_tag="POA77_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDI6364222.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(31165..32007)
                     /locus_tag="POA77_000745"
     CDS             complement(31165..32007)
                     /locus_tag="POA77_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI6364223.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(31994..32875)
                     /locus_tag="POA77_000746"
     CDS             complement(31994..32875)
                     /locus_tag="POA77_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6364224.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(32896..34188)
                     /locus_tag="POA77_000747"
     CDS             complement(32896..34188)
                     /locus_tag="POA77_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6364225.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(34202..35881)
                     /gene="ycjM"
                     /locus_tag="POA77_000748"
     CDS             complement(34202..35881)
                     /gene="ycjM"
                     /locus_tag="POA77_000748"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDI6364226.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(36094..36408)
                     /gene="pspE"
                     /locus_tag="POA77_000749"
     CDS             complement(36094..36408)
                     /gene="pspE"
                     /locus_tag="POA77_000749"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDI6364227.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(36483..36704)
                     /gene="pspD"
                     /locus_tag="POA77_000750"
     CDS             complement(36483..36704)
                     /gene="pspD"
                     /locus_tag="POA77_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDI6364228.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(36713..37072)
                     /gene="pspC"
                     /locus_tag="POA77_000751"
     CDS             complement(36713..37072)
                     /gene="pspC"
                     /locus_tag="POA77_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDI6364229.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(37072..37296)
                     /gene="pspB"
                     /locus_tag="POA77_000752"
     CDS             complement(37072..37296)
                     /gene="pspB"
                     /locus_tag="POA77_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDI6364230.1"
                     /translation="MGALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(37350..38018)
                     /gene="pspA"
                     /locus_tag="POA77_000753"
     CDS             complement(37350..38018)
                     /gene="pspA"
                     /locus_tag="POA77_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDI6364231.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            38185..39162
                     /gene="pspF"
                     /locus_tag="POA77_000754"
     CDS             38185..39162
                     /gene="pspF"
                     /locus_tag="POA77_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDI6364232.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(39282..40547)
                     /gene="puuE"
                     /locus_tag="POA77_000755"
     CDS             complement(39282..40547)
                     /gene="puuE"
                     /locus_tag="POA77_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDI6364233.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(40585..41865)
                     /gene="puuB"
                     /locus_tag="POA77_000756"
     CDS             complement(40585..41865)
                     /gene="puuB"
                     /locus_tag="POA77_000756"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDI6364234.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKNYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(41867..43354)
                     /gene="puuC"
                     /locus_tag="POA77_000757"
     CDS             complement(41867..43354)
                     /gene="puuC"
                     /locus_tag="POA77_000757"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDI6364235.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIANEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(43629..44186)
                     /gene="puuR"
                     /locus_tag="POA77_000758"
     CDS             complement(43629..44186)
                     /gene="puuR"
                     /locus_tag="POA77_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDI6364236.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(44213..44977)
                     /gene="puuD"
                     /locus_tag="POA77_000759"
     CDS             complement(44213..44977)
                     /gene="puuD"
                     /locus_tag="POA77_000759"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDI6364237.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            45189..46607
                     /gene="puuA"
                     /locus_tag="POA77_000760"
     CDS             45189..46607
                     /gene="puuA"
                     /locus_tag="POA77_000760"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDI6364238.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            46756..46920
                     /locus_tag="POA77_000761"
     CDS             46756..46920
                     /locus_tag="POA77_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364239.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            46910..48295
                     /gene="puuP"
                     /locus_tag="POA77_000762"
     CDS             46910..48295
                     /gene="puuP"
                     /locus_tag="POA77_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDI6364240.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            48429..48674
                     /locus_tag="POA77_000763"
     CDS             48429..48674
                     /locus_tag="POA77_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDI6364241.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            48987..50630
                     /gene="sapA"
                     /locus_tag="POA77_000764"
     CDS             48987..50630
                     /gene="sapA"
                     /locus_tag="POA77_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDI6364242.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            50627..51592
                     /gene="sapB"
                     /locus_tag="POA77_000765"
     CDS             50627..51592
                     /gene="sapB"
                     /locus_tag="POA77_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDI6364243.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSYSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            51579..52469
                     /gene="sapC"
                     /locus_tag="POA77_000766"
     CDS             51579..52469
                     /gene="sapC"
                     /locus_tag="POA77_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDI6364244.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            52469..53461
                     /gene="sapD"
                     /locus_tag="POA77_000767"
     CDS             52469..53461
                     /gene="sapD"
                     /locus_tag="POA77_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDI6364245.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            53463..54269
                     /gene="sapF"
                     /locus_tag="POA77_000768"
     CDS             53463..54269
                     /gene="sapF"
                     /locus_tag="POA77_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDI6364246.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            54337..54690
                     /locus_tag="POA77_000769"
     CDS             54337..54690
                     /locus_tag="POA77_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDI6364247.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            55058..55846
                     /gene="fabI"
                     /locus_tag="POA77_000770"
     CDS             55058..55846
                     /gene="fabI"
                     /locus_tag="POA77_000770"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI6364248.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            55991..57118
                     /locus_tag="POA77_000771"
     CDS             55991..57118
                     /locus_tag="POA77_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDI6364249.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLVPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            57186..59120
                     /gene="rnb"
                     /locus_tag="POA77_000772"
     CDS             57186..59120
                     /gene="rnb"
                     /locus_tag="POA77_000772"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDI6364250.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(59198..59302)
                     /locus_tag="POA77_000773"
     CDS             complement(59198..59302)
                     /locus_tag="POA77_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364251.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
BASE COUNT        14239 a        15563 c        14857 g        14776 t
ORIGIN      
        1 aatgccggac ttaagacgcg gatgcactgc tgtgtgtact gtagagtctg gcggatgtcg
       61 acagactcta tttttttatg cagttttaac tttgcagata gccgcattct cgccggaatt
      121 gcgtgattaa tgacgcggtc agtggtgtct ggcgactatc acgccgctga atcaaataat
      181 aggccgcttt cggtaaaatt tcgctaaccg gcaacatcac cagcccctgt ccgtgcaagg
      241 gatcgcagcc catttcttca ggcagtatgc tgagaaaatc gctttttgcc accagactga
      301 tacaggctga gaacgtctcg cagactacac cgacctgtgg cgtttgcgcc tgatcgtcaa
      361 gcaattcact caactgtttg tagtaactgc cgtgtggcgt cggcattgtc cagctgtaat
      421 ccagtaactg tttgatcgaa cgggcaccaa tggcggggtg tcccgggcgg caaaagatcg
      481 cgaattgctt ttccagtaat ttctcaaaag taaattcgtg gtcgtacggt ccctgataat
      541 aggtattgat ggtgaaatcc aattctccct gacgcaattc attaatcatc gacaccagtt
      601 gcccttccat aatgcgtact tttacctgcg gatgctgctg atgaaaacga gatatgacag
      661 ctggcatcag actgcgggaa atactggccc ccatgccgat attaatctgc cctgccagtt
      721 gcccttgtcg ttggcgaatt tcctcttgcg ctgcgcgcag ctcttcaaga ataagactgg
      781 cgtgctgata aaaactttca ccggcatcag ttaacgtcac gcctttacta cggcgaaaaa
      841 agagttgcgc cgctaatcct tcttccagct cctgaataga tttactcagt gccggttgcg
      901 acatattcaa cattcggctc gctccgcgaa tgctgccctg acgagccact tcaacaaaag
      961 cacgaatttg atgaattttt acctgaaaag tcatgacgcc accgataacc gttatttatc
     1021 agaccaaaga aactggcatc tactttaatg cagatgattg tgtcagggta atttatgaac
     1081 ggttaaaact gtgaaaaatc agttagtgat aagtaaaaac tatcgctacg tgaaccgggt
     1141 cacacttttt actgatgacg ggaaaggtta tggagtcttt gaatcaattt gttaattcgc
     1201 ttgccccaaa attatcgcac tggcgacgtg attttcatca ctatgcagag tctggctggg
     1261 tggaattccg cactgccacc cttgttgcgg aagaattgca gcagctcggc tattcactgg
     1321 cgctgggccg cgaagtagtt aatgaaagta gccggatggg actacctgat gaactcactc
     1381 tacaacgcga attcgagcgc gctcgtcaac agggtgcgct agcacaatgg attgcggttt
     1441 ttgaaggtgg tttcactggt atcgtcgcca ccctggatac cggtcgcccc ggtccggtga
     1501 tggctttccg tgtcgatatg gacgcgctgg atctcagtga agagcaggat gtcagccatc
     1561 gcccctaccg cgacggtttt gcgtcatgta acgccggaat gatgcatgcc tgtggtcatg
     1621 atggacatac cgccattggg cttgggctgg cgcataccct taaacaattc gagtccggac
     1681 tacatggcgt catcaaactg atttttcagc ctgcagagga aggtacgcgt ggcgcgcggg
     1741 cgatggtcga tgcaggtgtc gtagatgatg ttgattattt tactgccgtg cacattggca
     1801 ctggcgtacc tgcgggcacc gtggtgtgcg gcagtgataa ttttatggca accaccaaat
     1861 ttgacgcgca cttcaccggg accgccgctc acgcaggcgc aaaaccagaa gacggtcaca
     1921 atgccttgtt ggcggcagca caagcaactc ttgcactgca tgcaatcgcc ccgcacagcg
     1981 aaggagcttc cagagtaaac gtgggcgtta tgcaggcagg aagcggccgt aacgttgttc
     2041 ctgcctcggc gttgctgaaa gtggaaacac gcggggccag cgacgtcatt aatcaatatg
     2101 tttttgaccg tgcacaacaa gcgattcagg gcgcagcaac catgtatggt gtcggcgttg
     2161 aaactcgtct gatgggtgca gctaccgcca gttctccttc gccgcaatgg gtcgcatggt
     2221 tgcaaagtca ggcggctcag gtcgcggggg tcaatcaggc cattgaacgt gttgaagcgc
     2281 ctgcgggttc cgaagatgcc acattaatga tggcccgcgt gcagcaacat caagggcaag
     2341 cctcctacgt ggtgtttggc acacagctgg cggcaggtca tcacaacgaa aaattcgatt
     2401 ttgacgagca ggttctcgct attgccgtcg aaacgctggc gcgcaccgcg ctcaattttc
     2461 cctggacgcg aggtatctga tgcaggaaat ctatcgtttt atcgacgatg cgattgaagc
     2521 cgatcgccaa cgttataccg atattgccga tcaaatctgg gatcatccag aaacacgttt
     2581 tgaagagttc tggtcagcgg agcatctggc ttcggcgctg gaatctgcag gcttcaccgt
     2641 tacccgcaac gtaggcaata tcccaaatgc ctttattgct tcgtttggtc aaggcaaacc
     2701 ggttatcgcc ctgctgggag aatatgacgc cctggcaggt ttaagtcagc aagcaggttg
     2761 cgcgcaacct acatccgtga cgcccggtga aaatggtcac ggttgcggac acaatttgct
     2821 gggaaccgcc gcctttgccg ctgcaatagc cgtcaagaaa tggctggaac aatatgggca
     2881 aggcggcaca gtgcgctttt atggttgtcc tggcgaagaa ggcggctcgg gtaaaacgtt
     2941 catggtccgc gagggggtat ttgatgatgt ggatgcggca ctcacctggc acccggaagc
     3001 ctttgccggt atgttcaata cccgcacgct ggcaaacatt caggcatcat ggcgctttaa
     3061 agggatcgca gcacatgccg cgaattcccc tcatttggga cgaagcgccc ttgatgccgt
     3121 aacgttgatg accactggca ccaacttcct caacgaacat attattgaaa aagcgcgcgt
     3181 acactatgcc atcacagata gcggcgggat ctcgcccaac gtggtccagg cgcaggcaga
     3241 agtgctttat cttatccgtg cccccgaaat gaccgacgtg cagcatattt atgatcgggt
     3301 cgccaaaatc gccgaaggtg cggcattgat gaccgaaacc acggttgaat gccgcttcga
     3361 caaagcctgt tccagttatc tcccgaatcg caccttagaa aatgccatgt accatgccct
     3421 atcccatttt ggtaccccgg aatggaactc cgaagaactg gcttttgcga aacaaattca
     3481 ggctacgctc acccccaacg atcggcaaaa cagtctgaat aatatcgccg caaccggtgg
     3541 cgaaaacggc aaggtttttg cactacgtca tcgtgaaacg gtactggcga atgaggtcgc
     3601 tccatatgcc gccaccgata acgtgcttgc ggcatcgact gatgtcggcg acgtcagttg
     3661 gaaactgcct gttgcccagt gtttcagccc ctgttttgcc gtcggtacac cgctacatac
     3721 gtggcaactg gttagccagg ggcgaacatc tattgctcat aaaggaatgc tgctggcggc
     3781 gaaaactatg gcagcaacca cagtcaatct cttcattgat tcagggctat tgcaagaatg
     3841 ccaacaagag catcagcaag ttacggacac gcaaccgtat cactgcccta tcccgaaaaa
     3901 cgtgacaccg tcacctttaa aataacaaca acaacgcaaa cacaacaacc gaggaatgcc
     3961 catgagtatg tcatccatac cgtcgtcctc ccaatccggg aagctctatg gctgggtcga
     4021 aagaattggt aacaaggttc cccatccttt tttgctcttt atctatttga taatcgtact
     4081 catggtgacg acggcaattt tgtcagcctt tggcgtcagt gcgaaaaacc cgaccgatgg
     4141 tacgccggtg gtggtgaaaa acctgctcag tgtggaagga ttacactggt ttttacccaa
     4201 tgtgattaaa aactttagcg gttttgctcc acttggagcg atcctggcgc tggttttagg
     4261 tgccggtctg gcggagcgcg tcggcttact gccagcgcta atggttaaaa tggcatcgca
     4321 tgttaatgcc cgctacgcca gttatatggt gctgtttatt gcttttttca gccacatttc
     4381 ttccgatgcg gcgttagtga tcatgccacc gatgggtgcg ctgatttttc tggcggtggg
     4441 caggcatcca gttgcaggtt tactggctgc cattgcaggc gtaggttgcg gctttacggc
     4501 taatttactg attgtcacaa ccgacgtgtt gctgtcgggg atcagcacgg aagcggcggc
     4561 tgcgttcaat ccgcaaatgc acgtcagtgt aattgataac tggtatttta tggccagctc
     4621 cgtagtcgta ctgacgattg ttggcggcct gataaccgac aaaatcatcg agccacgatt
     4681 aggtcaatgg cagggaaaca gcgatgagaa actgcagaca ttgaccgaaa gtcagcgttt
     4741 tggtttacgc atagcaggtg tcgtatcgct actttttatt gctgcgatcg cgctgatggt
     4801 gatcccggaa aacgggatat tgcgcgatcc gattaatcac accgtgatgc catcaccctt
     4861 tattaaaggt atcgtgccac tgatcattct ttttttcttt gttgtctcgc tggcttatgg
     4921 catcgctacc cgcacaattc gacgtcaggc ggatttaccg catttaatga ttgaaccgat
     4981 gaaagagatg gcgggattta tcgtgatggt ttttcccctc gcccaatttg tcgccatgtt
     5041 taactggagc aacatgggga aattcatcgc cgtggggctg accgatatac tggaaagttc
     5101 agggcttagc ggcataccgg cgtttgtcgg tctggcgttg ctttcctctt tcttatgcat
     5161 gtttattgcc agcggttccg caatctggtc gattctggcc cccattttcg taccaatgtt
     5221 tatgctactt ggctttcacc cggcatttgc gcaaatcctc tttcgtattg ccgactcatc
     5281 cgtattgcct ttagcgccgg tatctccttt tgtcccactg tttcttggat tcctgcaacg
     5341 ctacaaacca gacgcgaaac tgggtactta ctattcgtta gtcttgccct atccgcttat
     5401 ctttttggtg gtatggctgc tgatgttgct ggcgtggtat cttgttggtc tgccgatagg
     5461 tccggggatt tacccacgtt tgtcttaaga gagaacggat gctgagatta cttgaagaaa
     5521 aaattgccac gccactgggt ccactgtggg tgatttgcga tgagcaattt cgcctgcggg
     5581 cggttgaatg ggaagagtac agcgaacgca tggtgcagct gctggacatc cattatcgca
     5641 aagaaggcta tgagcgcatt tctgccacca acccaggcgg tttaagcgac aagcttagtg
     5701 attattttgc cggtaatctt agcattattg atacgcttcc cactgccaca ggggggacgc
     5761 catttcagcg cgaagtctgg aaaactctac gcactatccc ctgcgggcag gtaatgcatt
     5821 acggccaact ggctgaacaa ttgggccgcc ctggcgcggc gcgtgccgtt ggtgcggcaa
     5881 acggatcgaa tcccatcagc atcgtcgtac cttgccatcg ggttattggc cgaaacggca
     5941 ccatgaccgg atatgcaggc ggagttcagc gaaaagagtg gttattgcgc cacgaaggtt
     6001 atcttttgct gtaaacatta aacaatttgt gccagcttgt tcacactttt atgtaaagtt
     6061 acccttaaca acttaagggt tttcaaatag atagacatat atttacatct aatatcggaa
     6121 ttctctgctg ttaaggtttg cttagactta cttgctccct aaaaagatgt taaaattgac
     6181 aaatatcaat tacggcttga gcagacctat gatcccggaa aagcgaatta tacggcgcat
     6241 tcagtctggc ggttgtgcta tccattgcca ggattgcagc atcagccagc tttgcatccc
     6301 gttcacactc aacgaacatg agcttgatca gcttgataat atcattgagc ggaagaagcc
     6361 tattcagaaa ggccagacgc tgtttaaggc tggtgatgaa cttaaatcgc tttatgccat
     6421 ccgctccggt acgattaaaa gttataccat cactgagcaa ggcgacgagc aaatcactgg
     6481 tttccattta gcaggcgacc tggtgggatt tgacgccatc ggcagcggcc atcacccgag
     6541 cttcgcgcag gcgctggaaa cctcgatggt atgtgaaatc ccgttcgaaa cgctggacga
     6601 tttgtccggt aaaatgccga atctgcgtca gcagatgatg cgtctgatga gcggtgaaat
     6661 caaaggcgat caggacatga tcttgctgtt gtcgaagaaa aatgccgagg aacgtctggc
     6721 tgcattcatc tacaacctgt cccgtcgttt tgcccaacgc ggcttctccc ctcgtgaatt
     6781 ccgcctgacg atgactcgtg gcgatatcgg taactatctg ggcctgacgg tagaaaccat
     6841 cagccgtctg ctgggtcgct tccagaaaag cggtatgctg gcagtcaaag gtaaatacat
     6901 caccatcgaa aataacgatg cgctggccca gcttgctggt catacgcgta acgttgcctg
     6961 atttttccgc ataactcact atccttctgt catatcatta aatttttctg atttattgat
     7021 ctggcagaag gttcatcact gtttcattca ccagatatgg gttaatcttt taattacaaa
     7081 ctgcgttgac agttgttgta aggagaccct gtatggctat gtatcagaac atgctcgttg
     7141 ttatcgatcc taaccaggac gaccaaccag cattgcggcg agctgtttat ttacatcaac
     7201 ggattggtgg caaaattaaa gcctttttgc cgatctatga cttctcatac gaaatgacca
     7261 ccctgctctc cccggacgaa cgtaccgcta tgcgtcaggg cgtcatcagc cagcgtacag
     7321 cctggatcca cgagcaggca aaatattatc tcaatgctgg cgttcccatt gaaattaaag
     7381 tggtctggca taaccgtccc ttcgaagcca tcattcagga agtgatcagc ggcgggcacg
     7441 atttggtgct aaaaatggcg caccaacatg accgtctgga agcggtgatt tttacgccaa
     7501 cggactggca tctgttacgc aaatgcccaa gcccggtgtg gatggtgaaa gaccagccgt
     7561 ggccggaagg aggcaaggcg ctggtggcgg tgaatctcgc cagtgaagag ccgtatcata
     7621 atgcgctcaa tgaaaaactg gtcaaagaga tgatcgaact ggcagaacaa gtcaaccata
     7681 ccgaagttca tctggttggc gcttatccgg taacgccaat taatatcgcg attgaactgc
     7741 cggaatttga tccgagcgtt tataacgatg ccattcgtgg gcaacatttg ctggcaatga
     7801 aagctctgcg gcagaaattc ggcattaatg aaaacatgac gcacgtagaa aaaggtctgc
     7861 cagaagaggt gattcctgat ttggcggagc acttacaggc ggggattgtg gttctgggca
     7921 cggtaggacg caccggtatt tcagcagcat tcctcggcaa cacggcggaa caggtgattg
     7981 atcatcttcg ctgcgatctg ttggtcatta aacctgacca gtatcagaca cccgttgaac
     8041 tggatgacga agaagacgat taacgactct tcaataactg acaaggcccg agttatcggg
     8101 cctatttttt acttactgct cttcgccgcc aaggaaataa attcccaacg ggattgccag
     8161 caacacagta aagaccaggc tataaacaaa aatcatcgat gactgaatca catacattga
     8221 tgtcgtccac tcagacagcg gaatattgta ctgttctata acgccgccga tagtcgcgcg
     8281 agtcacaacc gacgccgcaa taatgaaaac cgccaacaaa ccaaagagaa atttcttccc
     8341 tttcgctgac ttcagtttcg ccataatcat gatgtaatcc ttgtgcagac aaatttcttg
     8401 ttacctaaac gtaacaatac catgacattt caccgttgtc tgctctgaaa acctcacctc
     8461 ttagctggat ttacaatcaa cgcattgatt agttagctaa tattgctact tccttccctg
     8521 actattgatg atctgaatga attgtcgcgg taaaatagcc ccgccagggg tttccggcat
     8581 gacgacaatt atctggagga gcctgataaa acaatgatcg ctgaactgtt tacaaataat
     8641 gcgcttaatc tggtcattat tttcggtagc tgcgcagcat tgattctgat gagcttttgg
     8701 tttcgccgcg gaaatcgtaa aagaaaaggt tttttattcc atgcggtgca atttttaatc
     8761 tacaccataa ttatcagtgc tgttggtagc attattaatt atgttataga aaactataaa
     8821 ctcaaattta tcaccccagg cgttatcgat tttatctgta cgtccctgat tgcggttatt
     8881 ctgacgatta agctattttt gctgattaat cagtttgaaa aacagcagat taaaaaaggt
     8941 cgcgatatca ccagtgcgcg gattatgtcg cgcattatca aaatcaccat tattgtggtg
     9001 cttgttctgc tttatggcga acatttcggc atgagccttt ctggcttgct gacctttggt
     9061 ggtattggtg gtctggctgt cggtatggcc ggtaaagata ttctgagtaa tttcttttcc
     9121 gggatcatgc tctatttcga ccgtcctttc agtattggcg actggatccg ttcaccggac
     9181 agaaatatcg aaggtacagt agcggaaatt ggctggcgaa ttaccaaaat tacgaccttt
     9241 gataatcgtc cattgtacgt accgaactcg ctgttttcat cgatcagcgt agaaaaccca
     9301 ggacgaatga ccaaccgccg cattaccacg accattggtt tacgttatga ggatgcggca
     9361 aaagtgggcg ttattgtcga agccgtacgt gagatgctga aaaatcaccc ggccatcgac
     9421 cagcgacaaa ccttactggt ttatttcaac cagtttgccg actcgtcatt gaatattatg
     9481 gtttattgct ttaccaaaac cacggtatgg gctgagtggc ttgctgcaca gcaagacgtt
     9541 tatttgaaga ttatcgatat tgtacagtca cacggcgcgg actttgcctt ccccagccag
     9601 acgctgtata tggataacat tacaccgccg gaacagggtc gctaaatcag acatcactgg
     9661 cgttatccac aagggtaacg ccagttccca tcacatcaat gcttcacaat atacatagtc
     9721 cggctgtacg ccacatcttc cgggttatta atgggataac ctttcagcca cggcttgatt
     9781 aatcgtccat tggtatattg ataaattggt gcaatcggtg cttgctccat gaggattttt
     9841 tctgccgcgt tgtaatcggc attacgcgct ttaacggtat tttccgtcga tgcctgggcc
     9901 agaactttgt cgtatgccgg attgttaaag cgtgaaatat ttcctgaatg cgttgacgtt
     9961 aataatgtca ggaaagtgga gggttcatta taatccccca cccatgaggc acggataaca
    10021 tcaaaattgc cggtgttacg gctatcgata taggttttcc attcctgatt ttgcaattta
    10081 acatctacgc caaggttctt tttccacatc gatgctacag caatcgcaat tttttgatgg
    10141 ttttctgaag tgttatacaa aagcgtcagc ttcagcggtt tttgcggacc ataaccagct
    10201 gcgctcaaca aagtttttgc ctgcgcattc agttcttcct gactcatttg ttcaaacggc
    10261 gaaggttccg gcgtaaatcc ggcggtaaca tctggtgtaa aatgccacgc tggcttttcg
    10321 cccgttccca acactttttc ggtcatcagg cggcgatcta tcgtcatact taatgccagg
    10381 cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa acgcataata ataggtcccg
    10441 agctgtggcg gcgtataaac ctgccccgga atatccttca acagcttctg atacatattt
    10501 ttcgggaagg attcggtgat atcaatatcc cccgcgaggt aacgcttagt ggctgcggat
    10561 tcctgattaa ttggcaggaa ggtcactttt tgcagtaccg ttttggcgtt atcccaataa
    10621 tgggtattcg gtaccacgac cagtttttca ttgactacgc gatctttaag aacataagcg
    10681 ccattgccga tcagatttcc gggtttcgtc cactctttac cgctttctac gttggctttt
    10741 tgcaccggga agaaggcaaa gttagcggtt aaattcacaa accacggcaa cggtttatca
    10801 agctgaattt tcaaggtatg ggcatcaact gcggtgacgc caagctggtc aggcgtagct
    10861 ttaccatcaa taatcgcctg tgcgttgttg attcccgcca gcgcggcaaa ccatgcaaat
    10921 ggcgatagcg ttttcgggtc caccagacgc tgccagctgt agacaaaatc ttgcgccgtt
    10981 accggtgtgc catccgccca ttttgcgtta tcgcgcaggg taaaagtcca gatacggttg
    11041 tcattacttt tccactgagt cgcaacgccg gggacaatct cccctttctc gttctgattc
    11101 accagtcctt caaacagatc gcgaatgacc tgaatctctg gcaggcccac ggctttagcg
    11161 ggatccagcg acgcaggctc atctttaatg tggcgcacca gctcctgctt ctctgccagt
    11221 actgtgccgc tcggaacttc tgcagcatac gaaagagaaa tgctgctgac caacagcgca
    11281 caacacgtga ctgaaacaga gtgcttcata agtttccctc taaccgacat taaaattgat
    11341 gcgtaattat ttgtttcgcc ttaaagaaat gcaaacaact tccatcaaaa aagtgacaca
    11401 ggaagcataa aatgttttat gtatcaatat attaaatcag atttagtgta aataatcgac
    11461 gccaaaatgc ggcaaagcac atagaacacc gaacactacg ttcaggcttc gcacgttcaa
    11521 accagttttc ggtatcaaaa tcagcatctg tcgccattgt acgaagaata aaaaataacc
    11581 aaaacgttga aaggaactga gaatacgtta agagagaaat taaattaatg tatcagcaag
    11641 tagtgctaaa cagcaccttt atacttcagc ctatctataa ataatgagaa tgccgagcca
    11701 gcatgtcgac ggtgtggata gtacaggtga taaccgggta aatctggtgt gaatttatcc
    11761 aaaacacgta tcagtttttt ttctttaata gcccgctcaa cctgatcata aggtagatac
    11821 gccaatccat gcccatcaat tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc
    11881 tgaccttcca tgcgaacacg aacttcacgt ccaccgcgta ttaatctcca gcgatttgct
    11941 gtacccgatg tgggaagata caaattaatt gcctgatgat ctattaattg tgacactgac
    12001 gttggaacac ttcggcgaga aaaataatcc ggtgagccaa caatagccat tggtatatct
    12061 ggcccgattc gaatggcgat catatcttta tccatctccc cacccagacg gacgcctgca
    12121 tcaaaacgtt cagaaacgac atcggtcaaa ccataatcaa tggtgagctg aatatcaatt
    12181 tcaggatgcg atttcaggaa tgtgcgcatt gctggtaaca atatcgtttt tgctgcatgt
    12241 tctacagtag taatacgtat tgtcccggat gggcggttct gcagctcgct cagggatgcc
    12301 agggctgaat ctatgtcatg caacataggg ccaagaacag acaaaagatg ctcgcccgct
    12361 tcagttggaa caacgctgcg cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct
    12421 atacgacgca ctatctggct taaagctgac tgcgccatgc tcaggcgggc tgctgcacga
    12481 gtgaagctac gctcctctgc aacgacgaca aacgccatca gatcagcgat ttcttcacgc
    12541 ttcattcagt atctccatgc aatctattaa tcatttaaat gataggtcta atcatgattt
    12601 tgggtctaat caacttaatg atcaatatct atagtgatat caccaaccgt taaaacgaac
    12661 cgacaactcc atgaggaacg gaatgatgaa taataaagtc agcttcacta acagcaataa
    12721 tccaaccatc tctttgtctg cagtgatcta tttccctcca aaatttgatg aaacccgtca
    12781 gtatcaggca attgtggtat ctcacccagg tgggggcgtt aaagaacaaa cggccggaac
    12841 ctatgccaaa aaactggcgg aaaagggatt tgtcactatt gcttatgacg catcttatca
    12901 aggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca tacattcgta ctgaagacat
    12961 cagtgccgtg attgattacc tgacaacgct ttcttatgtc gacaataccc gaattggtgc
    13021 gatgggaatt tgtgccggtg caggatatac agccaatgct gcaattcagg atcgccgcat
    13081 caaggccatc ggtaccgtta gtgcggtcaa tattggttcg atgttccgta atggttggga
    13141 aaacaatgtg aaatctattg atgccctgcc atacgttgaa gcgggttcaa acgcccgaac
    13201 cagcgatatt agcagcggag aatatgccgt tatgccactg gcaccaatga aggagtccga
    13261 tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacccctc gcgcgcagta
    13321 tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta cctatgatgc
    13381 ttaccatatg gcggaagtgt acctgactca gccgatgcag attgtggcgg gcagccaggc
    13441 agggagcaaa tggatgagtg acgacctgta cgatcgggca tcaagccagg ataaacgcta
    13501 tcacattgtt gaaggcgcaa accatatgga tctttatgac ggcaaagcct atgtggctga
    13561 agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca tattcaaatc
    13621 cgcaatagtg atatggcctg gcatattgct gccaatattc agtttccacc aaattttgat
    13681 gagtcaaaac agtatccggc gattatcagc gtccatcctt ttggcagttg taaggagcag
    13741 acgtctggca atatctatgg caaagcattg gctgaaaaag gctatctcgt tctcgcttat
    13801 gacgccagtt tccagggaga atctggtggg gagcctcgct ggatagaaga tccgacacag
    13861 cgcgtagagg atattagccg tgttatcgat tatgcagtaa ctctgccata tgttaatgct
    13921 gaacgtattg gcgtgctggg agtgtgtggg ggtggtggct atgccattaa tgccgcgctg
    13981 acagaaaaac gaattaaagc agtagtgagt attacgggtg ttaatatcgg gcgtcttttt
    14041 cgggaaggtt tcagcaatta cgatccgatt ggcgcattga atgcaatggc gtcgcaacga
    14101 accaaagagg ctcgtggtgg agaattacag atcaacgaat tacttcccgc cagcctggat
    14161 gcagcaaaag cccatggact gaccgaacgc gatgtttatg aagcaactga ctactataaa
    14221 acaccacgcg ggcaacaacc gggaggtgcg acaaaaatgt tgttctcaca tgcacagaaa
    14281 acgctggcct gggatgcctt cgcatttact gaagtgttac tgactcagcc ggtgatggta
    14341 gtagttggtg aaaaagtcgg tgccttcggt gcttatcgtg atggactgga agtctatggc
    14401 cgggcgatgg tttcgcagga tcgtcagtta gtttcattac ccgatttctc acattatgaa
    14461 ctgtatgaca aaccggaagc agttcagaag gcgctggaaa aagttattcc ttttttcaac
    14521 acccatctgg gataagtttc atgagaaata ttatgaaaaa tgtactgatt cttggtgctg
    14581 gcggtcagat agcccgccat gtgattaatc aactggcaga taaacaaacc attaaacaaa
    14641 ctttatttgc ccgtcagcca gcaaaaatcc acaaacccta tccaactaat agcaagatta
    14701 ttatgggaga tgtactgaat catgcagcac tgaagcaggc catgcaggga caggacgttg
    14761 tgtatgccaa ccttacgggg gaagatttag atattcaggc aaattcagtg atcgctgcga
    14821 tgaaagcctg tgatgttaaa cgtctgatct ttgttctttc cctcggtatt tatgatgaag
    14881 ttccaggaaa atttggcgaa tggaataatg cagttattgg tgagccccta aaaccattcc
    14941 gcagggctgc ggatgcaatt gaggcttctg gactggaata tacaattctt cgccctgcct
    15001 ggctgactga tgaagatatt attgattatg agcttacctc acgcaatgaa ccattcaaag
    15061 gtacgatcgt atcacgtaag agtgtcgcag cattaatcac agatatcatc gataaacctg
    15121 agaaacacat tggtgagaat ataggtatca atcaacccgt gactgatggt gacaagccat
    15181 tattcatgta aatcaattac atagttacca tcgtaatgta ctatgtgcag tacgatttat
    15241 taaacttatt aaggtgaact gatcaatccg atgcgattaa catgtgtaga accagtaaga
    15301 ataaaatacg ccatgtatat aacattacga cttacatcta agtcagcata attctaatat
    15361 ctgcacatca ataaaaatgc aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca
    15421 tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacactgc ctgtaatact
    15481 tcctgaacca gagacgatca tgaccgtaaa ccgcccacgc gccgaacgcg gcgcatttcc
    15541 gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct ggtttccggc
    15601 cactgcagcc agtcgcgaaa gtggtttgat tctggctggc actcacggtg atgaaaactc
    15661 ttcggtcgtc acgctctcct gtgctctgcg gactttgaca ccttctttgc gccgccatca
    15721 tgtagtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca atgctaatgg
    15781 tgtggattta aaccgaaact ttccggcggc gaactggaag gaaggtgaaa cagtttatcg
    15841 ctggaacagc gccgctgaag aacgtgatgt cgtattgctg acaggtgaca aacctggctc
    15901 tgaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacccg cctgggtggt
    15961 ctcattccac gatccgctgg cctgtattga agatcccaga cacagcgaat taggcgaatg
    16021 gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa cacctggctc
    16081 tttcggtagt tggtgtgcgg atctcaacct gcattgcatc accgcagagt ttccgcccat
    16141 ctcctccgac gaggccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcactggca
    16201 tcctaaagat gcaattcgcc tgtcgtgaac tgaagcgccg gttccacatc taccgccagc
    16261 caggtcggtc cgtcaagatc gacgaaactg acctgcggca ccagcggtaa agcggcgcta
    16321 atggcccgag aggtacacaa catgcagccc agcatcagac gaaaaccttg tgcacgcgct
    16381 tcagtcgcca gcgccagcgc ttccgtcaga ccaccggttt tatcaagctt aatgttaacc
    16441 atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact ttcatctgca
    16501 caaatcggta acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaagcggt
    16561 tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttct
    16621 gcacgccagg attcatttgc gtcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt
    16681 gccaccatcc gctcactgat aagatggtta tccagcttca ctttcagtaa tttcgcgcct
    16741 gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat gactaccgtc
    16801 tgtgcagtgg tgactgtccc gggaagcgtt atgccgatca aatcagccag cgattgctgc
    16861 tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca
    16921 gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcgg cacgacgctc
    16981 ataatttgcg ccattaccga ggcatcactt tccccataac gcggatacgg cgtgcattcg
    17041 ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga
    17101 cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa taccttaacg
    17161 gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg
    17221 ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag
    17281 ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc
    17341 ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc
    17401 accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc
    17461 ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caactgagat cgacaacacc
    17521 gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa
    17581 ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagcctac
    17641 ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt
    17701 gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat
    17761 tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca
    17821 tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact
    17881 tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat
    17941 aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg
    18001 aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa
    18061 tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag atagcgttct
    18121 ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc aggtcagcgg
    18181 ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta
    18241 acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca
    18301 gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc agattcttct
    18361 gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga
    18421 aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta
    18481 tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg
    18541 gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca cagttcagcg
    18601 ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca aagagatctt
    18661 tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg
    18721 cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg aaggcgctga
    18781 cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca
    18841 ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct
    18901 ccatcaggaa attctgccca ttaataacga catgctcgtt atgggaatct tgcggttcgc
    18961 tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca
    19021 ggcgatccaa tttttgcgca aaaagctgac agctcgccgg gttcgccata tccactttgc
    19081 ttttcatatc gacagagagc acaggttcgg gcaacgcctc cagtaacgcg ctcaacgcca
    19141 gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca cctgcaatac
    19201 ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca aaattgaggt
    19261 agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta
    19321 gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg
    19381 gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg
    19441 aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga
    19501 tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagcg
    19561 tttctttcac ctgaccgata agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt
    19621 caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt gccgtcagaa
    19681 gtccagcacc aattccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca
    19741 tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca
    19801 ataccagctt aaacagacgc aaacggctgt aataccctag ttcaatgcca tacagcgtgg
    19861 cgatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca
    19921 gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggctgatt tcgcgccgcg
    19981 cctgggcatc taaaactggc tggaccaaat gcgcatacaa actgaccact tcacggtcgt
    20041 tttgcgtttc atggattgag gcataccagc gttgcagcgc tggatgagac tgatcaatac
    20101 ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg tggctgtgca
    20161 acaaatcgcg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc
    20221 actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac
    20281 ccagcgccac ccagtcctga gtttgccagg cattcattgt ccactgtaca ccctgcccga
    20341 caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt
    20401 tcggacgtaa cgctgcatcc attaccgctt caacttgccc ctcttcttca ggcgcttcat
    20461 cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga
    20521 attttggatt ctgatcgacc tccagcggac cgtcgaaatc aatacgtggt tttaacggtt
    20581 cggtcatcgc aatttatctc ctattaaaaa ttcaagcgcg gcatcaagac gaatatgcgg
    20641 cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga acccttgctt
    20701 gtcccagaac gcctgaccgg gcaaacgcgc aggaacttca ccgggataaa cggtgagtgg
    20761 cgcaccatcg ctgagccggt taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat
    20821 aatgccgctg gtggtcgcct gcaccgaggc cagccccagg caatccatac taatcccttc
    20881 aaacgccgca ttttgccagg catcctgaat cagttgttgc agtaatgaaa ccatattggc
    20941 gtgctgatcg atggtcacat ggtccgcttt agtggcagca aacaatagct tatcgataac
    21001 cggcgaaaac aaacgccgga acaaggtacg ctgcccgtag tggaaacttt gcatcagctg
    21061 cgtcagtgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag gttgcaggca
    21121 atcaaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga accccttcac
    21181 caccttctcg cagtaataat taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc
    21241 ggcctgcgcc agtttggact cgccccaggc atcgacatcc ggccacggga agaattgcag
    21301 cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag
    21361 cccctgctgt ttacagtggt ggagataatc ggtccacgcg gcggcaatgt ctgccagccg
    21421 gttttcgtcg gcaggcgcta gcgggtccag cccttcgcac atcattcgcc atttgaccga
    21481 ccattcgccg cgctgaccat tgagtaagcc cgtcatctgg cgtgaccagc ttaaatagtc
    21541 ctgcgccagc atcggcaggt cgagcaacca ttcgccggga taatccacaa tctccagata
    21601 cagcgtggag gtgtccttga agtggcgtag cagcgaatcg ttcgatttaa aacgtagcgc
    21661 caggcggatt tcactgacgc cgcgcgttgg cgtcggccag gcgggaggat cgccatataa
    21721 ctgcgccagt ccttcatcat atgtgaagcg cggaatgcca aagtcacgct gaggaatgcg
    21781 ttttacgccc agcaggcgct cttcacgcac cgcgcttaac agcggcaagc gtgctccggc
    21841 gtgaatattg agcaactgat tgaccatcgc agtgataaac gctgttttgc cgctgcggct
    21901 tagtccggtt acggcgaggc gcagatgtct gtcgacaccc cgattcacca gcgcattaag
    21961 ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat
    22021 tcgtaaggat atgaatgtgg gggccaggcc attgattatc aactgcccgt gttgtcatcg
    22081 acgggcgaac tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc
    22141 ccctactatt tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt
    22201 gctgaataag caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga
    22261 agaattacag tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc
    22321 cattatggtg atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga
    22381 agttttgcat gccatcaccg ccaaagctga agaggaaggt ttcgacgtga tcttgcagac
    22441 gtcgcacaat ccggcagaag acttacaaaa atgtgaaagc aaaattaagc agaaaatgat
    22501 taaaggcatt attatgctaa gttcgccagc ggatgagtca ttttttgccc aacttgataa
    22561 atacgatatt cctgtcgtgg tgattggcaa agttgaaggt caatatgccc atgtttattc
    22621 tgtcgatacc gataattttg gcgacagcat tgcgttgacc gatgcattaa ttgaaagtgg
    22681 gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc catgtttcag tggatcgggt
    22741 aaatggttat aagcagagcc tggctgcgca taatattgca gtgcgtgatg aatggattgt
    22801 tgacggcggt tatacccatg aaacagcact gcaagccgcg cgggaattat taagccagtc
    22861 accgttgcct gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc
    22921 ggcggcagag aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga
    22981 aacgctgtcg tttattttaa cgcctgcgcc aggcggtatt gatatcccaa cacaggagtt
    23041 aggtcaacaa agctgtgagt tattattccg cttaatttct ggaaaaccgt caccacagaa
    23101 tattaccgtt gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg
    23161 gatgcgatgc tagcgcatct tatccggcct acatttatag ttcggataaa gtactcgtgc
    23221 cgcatccggc aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg
    23281 gaatttatcg ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac
    23341 ggaaagcccg ttctggaaat cataaccgta aaataaacct acacggttaa aatcatggcc
    23401 ttcacgttca atatcatcct gccagtccca gttactccag cgatccagcc caatgcgcgt
    23461 atacggcgtc accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgcgt
    23521 agaaaactca ccgttattgc ggctgtcgtc catattaaag ccgcgctcga gataatagtt
    23581 cactcgcaag gcaaccgttt cgttgaaggt atattgcagg cctgtttccg tttcgactcg
    23641 ggtatcagcg taaccggtcg tgttcagatc gttggcaaat ttatacatcg acaaccaacc
    23701 gttgaaacgt aaatcgtcag tcagtttcac atcccagtct ggcgcgattt tccagcgctg
    23761 catattcgcc gtgtctttac ccggttcatc aacgtagtga taaccataat tacggaaacc
    23821 gccggtcagg ccgaaactga aatcatcgtt ttcgaggaac tgataatgca cctccagctc
    23881 agggcgatca aaccacgttc cacgtttacc cgcgctataa tctaccggcc cttcctgata
    23941 ataggccaga gcaattctcc acggcccgtt ggcggcatta aaatagactg aaggctccgc
    24001 cagcccatcc atatcttcgc cataaccctc gacgttttct atttcgtaca tcgcgccgat
    24061 attaaagtgc cagtcgttct tttcctcggc ctgtgcgcag gccattcccg cacacatcac
    24121 cagtgcggta cagggtaata actttttcat tattgttcct tgtattattc tgggggaatg
    24181 ccttccccct gtatttagcg aagtgctatt tccgtttctg catcaaagaa atgacatttc
    24241 gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa cgcaccagca
    24301 cggaccacta actcgtgccc cccaaccgtg gtgtagagca taaattccgc accggttaat
    24361 tctgccacgc taattttggc ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct
    24421 tccggacgta ttcccatcac gatgggctta tgcaaacttc cctgtgtttt cagaaccgct
    24481 aatttctctt cgggaatggt taatttaagc gtttccgtaa cgaatttatc gccatcgatc
    24541 gtgccacgaa taaaattcat cgccggtgat ccaataaatc cggcaacaaa catattcgct
    24601 ggttggttat aaaccgtttt cggcgcacca acttgctgaa caatcccatc cttcataatc
    24661 acaatccgcg tcgccatggt catcgcttcg gtctgatcgt gggtcacgta gatcatggtg
    24721 gtattcagct tctggtgtag cttgctgatc tctgcgcgca tttgcacgcg cagcttggca
    24781 tcaaggttag agagcggttc atccattaaa aacacgcccg cttcgcgcac aatcgcccgc
    24841 ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac
    24901 tcacgcaggc cgagaatttg cgccgcccag ttcacccgct catcaatcac ctctttggcg
    24961 attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac
    25021 gcgtagttct ggaacaccat cgctatattg cgtgctttgg ctggaacgtc attcattcgt
    25081 ttgccgtcga tcaacagatc gccgccgctg atctcctcaa gcccggcaat catgcgcagg
    25141 gtggtcgact taccgcagcc cgacgggccg acaaacacga tgaactcttt atcggcaatt
    25201 tccaggttga agtccttcac cacatgcacc tggttatcgt agattttttg aatatgttgt
    25261 aacgaaagct gagccatgtt gattcctttg ctatacgttt tgccagaagg ccgataaccg
    25321 cgaccaggtc agagagtccg ttgaaggcaa cagtaattgc gccccggtta agcccgcgcc
    25381 gatccccacc gagcgcatac cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc
    25441 aatgccgata catgcctgcg gcggcacgcc cagcccggca caggcggcga gaaagatttc
    25501 cgggtccggt ttcgagtttt taagttggga agcatccgcg cagaaggtga aaaactcgcg
    25561 cagctccagc gccgctaaaa tcgtcggcgc attcagggag acagaagcca gcccaaccgg
    25621 gatctgctgt gcacggagat ctgccagcaa agagcgaatg ccgggtagaa cagcgctgac
    25681 cgtcaactcg cgcagtgagt ggacatagag cagatttttg cgatacgcca gttgcgccct
    25741 ctcctgcgag ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc gcagagactc
    25801 atcgcggctg atccctttta gggattcgtt aaactgcgca tcaatgctga tgccaatttc
    25861 agcggcaatc tgctgccacg cctggaaatg cagatgcgcg gtatcggtga ttacaccatc
    25921 cagatcgaaa attacccctt gcagtttcat tcatcctcct gatgtgtggt agcggtccca
    25981 ttgaagggca aaataaaatc acccaaacag aaaacagatt cttcagccac ggatataagc
    26041 tgaccgttca aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtca
    26101 agagtgacct gtaattcgca gccctgccag aacagaggga aagacaactg ttgccactgc
    26161 tcaggtaacg ccggattgag atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc
    26221 tgaatcgccc ccagccagat agcgccagtt gcggcagcat ggataccatc atcacaacta
    26281 tgcggatcag caccaagatc gatttcagtc ccctcgcgcc agaactgata actttgggtc
    26341 agcaggccac agcgtgcggc aacaatgccg tggattgctt tacttaatga cgagtcgtga
    26401 atagtgcgcg gttcataaaa ttgcagattg gcaagacacg atgccgctga gaactgctcc
    26461 ggcagcatgt aattgagcat caccacatca gcttgtttga ggatttgcat ctcgttcact
    26521 tctgcgcgtg aataatccag cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc
    26581 agattaatcg ccggcttagc cataaacgaa tcatcctgcg gcaaaacgcc gtcgggctga
    26641 atttctggca gccgcagttc tttaaggaac atttcggcgc gatggataaa cgcatcgtcg
    26701 ctacagccaa actggcgggc aatactcagc gcctgctgga cgttgtaata cgccatatag
    26761 ctggtgaagg cgttattatt gacatgttcg gtatattcgt caggcccaat aacatcatga
    26821 atttccagac ggtcgttaac cctcaccgcg cggctaatcc agaactttgc agtttccaga
    26881 agtagcgcca tgccttcatg cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga
    26941 ataaccgccc aggcgatatc ggccaccaga tgatgttccg cctgcgccga ggccactttt
    27001 tgccgcagcc cggtgcgaat gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg
    27061 cgcgcgcttt cccacggaaa tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc
    27121 gcgcctggca agttgtgcca gcgataacgc agtaaacttc gggcaaccgt cggatcgcta
    27181 aacagatgaa acggtaacaa aaatacttct gtatcccaga aaacgtggcc tttgtagcct
    27241 tcgccggtta agccttttgc cgcaatgctg ctgcgctcgt cgtgggcagg cgtcatgatg
    27301 cgcagatgat aaagcgcata atctaacgct tgctgatcgt gcgcttcgcc gccatttacc
    27361 gtgatacgac gtttctgcca ccattgacgc cagttttctg ttgatactgc aagaagttgg
    27421 tcgtaactct gctgcgcgca catttcaagc tggcgaagcg acgcgctgcc ccactcgtct
    27481 aaaacggctt gcctgtcatc ccgccagtcg atccacacca gtttttgcaa cgtcactgtc
    27541 tcgcctgcat gaagctgcgc actggtatgt tgctgcaaac ggcgctcttt ggcggtatag
    27601 cattgctgca catcaccgct caccatacag caacagctga tggccacatc actgcgtcca
    27661 tcctgggtgg tgtagatccc ctgcatcaga tgctgaccaa acacccgcac ctgggtttcg
    27721 tcgagatgtt ggcgaccgtg gttggtttgc gtggcgtcga tacctgttga aatcagcact
    27781 gacgcgtcgg cgtccagtgg cgtaatagtg atttccagca caatgagcgg cagttggtct
    27841 gccgaaacaa agcgacggct ggcgatggtg taacctgtgc cgttgctggt acgccagaca
    27901 acgctacggc gtaattctcc actggcaaag tcaagttcac gctgccaggc ttcgcgggat
    27961 aacgagaaaa cctcaccatt tatggcaatc tccatcccca agatatcagg caggttcacc
    28021 agttcgttga tttcaccttt tcccgcccga tgatacagcc ccgccagata catccctcgc
    28081 gtctggcgcg tgtaatcttc ttcatggctg gcgcgaagcc caagatagcc gttcccctgc
    28141 gccatcagcg atgcatactt gttcagggta tgctggctga aatggggttc tgataacgtt
    28201 actggcctgg tcataattcc acacgcgtcc ctgtttctgc ggattgatac aacgccgcca
    28261 ccagttgctg gatgatgtac ccctgctcgg cgtcggctat catcacgggc ttgccctgta
    28321 cgtgattgat aaaggcttcc atgctgcgca aatggcggtt gtcgtctgcc agttcccgtt
    28381 gcatcagcgt cattaattca ccgttgttat cggtgtagat atgtgctgga aacagcgtcg
    28441 caccggcttt atcaccacaa aagctgacgt tcataatcga ctgttcgcgg atgttgagtg
    28501 caaatgacgt ttccagccac agaatgccgc cgttatgaaa ttcaatggtg ccaaataacg
    28561 aatcttcgac gctgtaagtt gccggatccc actcgccaaa ttgaccgcag ctcttttgcg
    28621 tgccgatctt ttgaaagcta tgcgcattca cgcttttcac cgccggaaag cccagcacat
    28681 acatcgccgc atccagcata tgaatgccga tgtcgataag cgggccacca ccctgcagtt
    28741 ctttattggt aaagacaccc cagccgggaa cgccgcagcg acgcagggcg cgggcggtgg
    28801 taacgtaaat ctctcccaaa acgccgttgg tcacctgttc acgcagctgt tgcgtatcga
    28861 gcgcaaaacg gtggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    28921 acatttcccg cgcctgttct ggcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    28981 ccgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag cagacgctaa
    29041 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattac
    29101 cgtatttatc tgccagcgcc tgcgcctggg aaaggcggct gtcacagaca gccaccagtt
    29161 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcctgcgc
    29221 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    29281 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttcc gcccggatac
    29341 gcccttcgta cactacatag ccctgatagt tatccgcgcg cagctgttca aacagcgcgt
    29401 ggaaatccag ggtgccgctg cccggctggt agcgatggtt atccgcaata tgcacatgac
    29461 cgagcaggtc gcggttgtca tgcagcgcct gcgccaggtt atcttcttcg atattcatgt
    29521 gatagaaatc gccgataatc tgtacatgtt taagatcgtt ttcgacgatg taacggcggg
    29581 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    29641 cggttccggt acgcgcggcg acctgttcca gtacgtgcag ggaatcactc accattttgc
    29701 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    29761 acgctgccgg aacgacgata cctttacccc ccacttctgc cagcgcttcg agaatgcgtt
    29821 cgatctgctt taagccatta agacgacgct cttcgataaa gtcgccaatc caaccgtcat
    29881 agccaccgca ggcggtggtc accggtaaac cggtttcttt gattgctgct ttgacttctt
    29941 cgagattgtt gaccagcagt ttgccgtcaa tctcaaaacc atcgaagccc atctctttga
    30001 tataacgaaa tttctccaga atgttttccg gaaagaatgc ctgattctgt gtgcctattt
    30061 tcattttttc attcctttga attaaaacgt aacgcccatt ttgatgctct gttccggatg
    30121 ctgatcgaca tactgcatgt agctttccgg gctattggca aaggtcacta ccgggtcgat
    30181 taaatcttcg caattgagat aaccgttcat cagcagttcc cagcaggttt cttcaatacg
    30241 cttacggctc cagcgcggat aatccgggtt cggttcgctg cacgcgcggg agaaaacaat
    30301 tttggcgtta ttgaaatgcg cttcgcgtcc gaggttaaaa ccttcagcaa atggtttggc
    30361 aaacgcgaca taagagatgg tgccgccata agcaaggcca cgcagcgccg attgcagcgc
    30421 gtcggcgtaa ccgctggttt cgataatcac atcggcaccc tgcttgccgg tcagcgtttt
    30481 gatctctttt cctacgtcag tgccaatggg gttaaggcag aaatcagcac cgtggcgacg
    30541 ggcaatatca cagcgatagg cgataggatc gacgccaatc accaccgacg cgccagcgcg
    30601 tttcgccagt tggatggcta tttgaccaat cgcgccaagc cctaccacca ccacaaaatc
    30661 acccacgcgc acgttggcat cgcgcacgcc gctcatggca aactgcgccg ggtcgtaaca
    30721 aacggcgttt ttccacgagc tgccttgcgg cattttgcgc agcttgtagt tattcaccgc
    30781 gttaatgatg accgtctcgg agagcgggcc gtagccgcat acgctgtcgc ccaccgcgta
    30841 gtcggtaacg tcgctgccgc actcgataat gtcgccaacc accatgttgc caagctggaa
    30901 tttgccaaac tcaatgccgc gcggcgcatc tgccggacgc ggagtgaaca tctgccattc
    30961 gccgttaaag tcttcatcga taaacgggct ggcggcgcgg aagtcgacca cttccgttcc
    31021 gtgtttcggt gcgccgaaac gcacgcggat tttcacttca ttggctaaaa tcgcccgatc
    31081 ttcatactca accagcgcag caacacgcgg tgctgtggct actaactttt tcatttaaaa
    31141 ctccttgtaa agaaactggc cgctttagcc cttcacgcca ccggcggtca aaccactctt
    31201 aataaaacgt tccgaaagcg catacataat caccaccggc aatgcggtca ccagtgaggc
    31261 cgccatcatc cgtccccaga tgtaatctgg cgtactgaac agcgcgttca ggcccaccgg
    31321 taaggtgaaa ttgctggcgc tggagaggaa gatcgacgca aacagatagt cgttccacgc
    31381 caccataaag cagtagacga acacggatat cagcccggac atcgccaacg gcacggtgat
    31441 gcggaagata atttgcagac ggttgaggcc atccatcatc gccgcttctt cgatctcatc
    31501 ggggatggtg tcgaagtagc ttttcagcat aaacacggcg gtgggcagag tttgcgtcac
    31561 catggtgatg atcagcgcca tctcagtgtc gtaaatgccc agcgcggtga tgattttgaa
    31621 cagcggcacc accagcaaaa tgccagagaa catatacacc gtgtaaaagc tggcgttgat
    31681 ggtcatccga cctttaaagc gcaggcggga aagcgcgtaa gccccaagaa tgccgaggaa
    31741 taccgccacc acggatgaaa ccacagacac caccagactg ttacggaagt agtcgacaaa
    31801 cgggaaaatc atcgggttaa aaatgtcgac gtaatgctcc agcgtccact gctgcggcag
    31861 cagcgtagga tgcagtgaga tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac
    31921 aaaaaatgga aacagcgtga tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg
    31981 actgagtgtg cgtttatttg ttgccattga ggttcacccg tttcctggtc agcagaatga
    32041 cagcgaaaat aatgatgaag agcaccaccg aaatcgccgc cgctttccct aagtcattaa
    32101 aggcaaaggc ggttttgtag agatagacgc cgagaatatc gaccttggtg gtcagcaaat
    32161 aaacatccgc gaacatgtag aacatccaga tggtgcgcag ggtcaccacc gtcgccagga
    32221 ccggcataat cgcgggcagc gtgacgatac gaaaccgttg ccaggcatta gcgccatcca
    32281 tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga aacgagataa
    32341 aggcatacgg gaagtagcgc cagatggcga acagcaccac cagcacaaag ctactgcccg
    32401 gattatcgaa ccacagcggt gcctgctcat agagatgcag aagatcgacg ccgaggtagt
    32461 tcacaatgcc gtagccgttg ttgaacatgt atttccaggc gaacaccagc gaaatggacg
    32521 gcgttacgta ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg aactcgcgat
    32581 tgaaaaacat cgccaccgcc agcccgagca cggtgctgcc cgccaccacc agcgcggtgt
    32641 accagaccgt catccacagt gagtgccaga agccgggatc ggagaggata cgcacatagt
    32701 tgctcacccc aacaaaggtt gactcgatgt tgggattgag cggcagacgt aaaaaactaa
    32761 tttcgatatt cgacaccatc ggccacgcca ccagaccgcc cagcagtaat aagctgggcg
    32821 cgagaagcaa cagcgcaaag ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg
    32881 attcctgtcc tgggtttagt gctgttcgac cagttcatcc agctttttct ggctcgcttg
    32941 cagcgtgcca gggagatcgg ctttacccac ggtgacgtta tgcaccatgg agctcaccac
    33001 gccagacccc gtcacatcac ccatgcgggt aaagttttta tcccctactg caccaaagac
    33061 ctgaatattt ggcagttcac cgattagctg attcggtagt tcacccagcg ccttaataac
    33121 gtcgttgtct ttccaggtgg cggtagtgac aaccgcttta ttcaccggca gcgcagcacc
    33181 tggcgacatc atcacccagt cggcaatgtt gtctgcctgc tccataaagg tgacaaattt
    33241 ttctgctgct tccgtctctt cggtcttttg cccggcggta atggtcagcg aggtcaacat
    33301 gccgtagacc gcagagtttt tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc
    33361 ttctttaatc acagccggaa ggatataggt ggagtaaatc gccatcggcg cggtgccgtt
    33421 cataaaggcg tctttcactt ccatgatgtc gttagaaccc ggcatagtgt tggcagcaag
    33481 gtcgcggtaa taggtcagtg cctgcatcat ctctggtgta tcaagggtga ttttgccttc
    33541 ggcgttaaag acgttagcct ggttggataa cgcaaactgg gagaaggatt gttccgtcaa
    33601 cacgctttct gctgtaggca gcgcaatgcc gtatttttta ttcgccgggt cattcagttt
    33661 ctgtgcaacg tccagcagct gttgccagtt tttcggctcc tcaagccccg cttttgccag
    33721 cacatctttg cgataccaga taccgccaat ccaggcgctg acaggaacac cggtccatgc
    33781 gctaccatct tcggtacgca caatacgcag tacgccatcg taaaacgcgc cttcaccaac
    33841 gttgctgatg actgtggcaa ccgctttgcg atcgataagc tgctctttgt ccatcacttt
    33901 ggcgtagtca tggctggttt cgatcacttc cggcagcgaa ccgctacgtg aaagagtaat
    33961 gactttagtg ttataggcat cttcttccac gggcacttgc ttaacggtga tgccagggtt
    34021 ttctttttca aaacgggcaa tcaatttact gataacggcc tggcgctcct gctccaccga
    34081 agaatgcata aattcgatgg atacattcgt tttattttct tctttacagc ctgaaatcag
    34141 ggcgcaggaa accagtgctg ataacagcac gatttttgat ttaatcatga gatgttcctt
    34201 tttatttaat ccacataacc tgccaggggc gcagcgttat ttcattaccc aatatatcaa
    34261 cttcactaat cagatcccga ccatgcagat tagtaatatt tacatgctga atatttttac
    34321 tgacattaaa caatccagtc agacaattac catcagcgtt acttctttga atacgcatta
    34381 cggatgaatt aatcgtatca atggtaaaat tattatccgg atgaaactcg ttatggctgc
    34441 gacgaagtgt aattaaacgc gacaactcat gatataccgc atgccttaat gtagcttcat
    34501 cgttcagttc tcgggttatc tctttactgt gatatttttt acggttaatc gcacggttat
    34561 atccgagttt ttcgacacct gcgtaatcat tacgcgagcc aagaatactt tgaatatata
    34621 tcgctggcac accggggaaa cttaacaaaa tcgcatgggc gagaataaac ctggcgcaac
    34681 gttcttcatc gctactctca cggcggctta acgcatccat ataggtcaca tttatttcat
    34741 acggactgcg tgtaccgtcg ggattatttt tccagtttac taatgcacct tcctgctgta
    34801 acgcctcgac cagctctaat atttcgcttt caggcaacaa gccccgtagc gggtttagcc
    34861 cgatgccatc gtgagaggcg aggaagttaa accaggtggt gttgctggaa ggtagtgtca
    34921 ggttttgcgc ccacgcacaa agcgcctcaa cgttctgttt ttgcaccgca tgcagcacca
    34981 gtggcggcag cgagaactgg tacaccatat gtgcttcgtc atcgcctgcg ccaaagtaag
    35041 caatgttgtc tttatgcgga acattggtct cggtaatgat cactgtacct ggcgcaacgt
    35101 tatcaataat cgaccgtaac agtttgataa tcagatgtgt tttttccaga tggatgcagc
    35161 ttgttcccgg ctctttccac ataaagccaa cggcatccag gcggacatac tcagcacctt
    35221 tcgcaaggta acagagtaaa acatccacca tcgccagcaa cacttcaggg ctacggtaat
    35281 tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg gcgcgttgaa tgatcgcgca
    35341 tctggaatgg cgttaataac ggtaacgcac gcgggcgagt gacggcgctg agatcggttt
    35401 gcgggtcaac ggcaataaaa aaatcttcaa aacctggatg ctgttgtaaa tagtttttaa
    35461 accattcact ttttgccgac atatggttgc agacaaaatc aaacattaaa tgactgcatt
    35521 caccgagttg ctgaatatcc tgccactccc ccgcttcact ggcgacctgt tgataatcaa
    35581 ttaccgaaaa gccatcatca gatgaccatg gataaaacgg caacaaatga acatgtgaaa
    35641 aaatgctttg cagccattga tggtaaaact gattaaatgt gggtaatggt tttaaatcat
    35701 tgctgtgaaa ttgatcggca taggtaatta acacgacatc actttcatcc cagtgttttt
    35761 tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat
    35821 gagttgcggt aaatgttcca ccgtagattt cgtccaggtg atccgtaatt ttctgtttca
    35881 tgttgccttt ccgtggtagc ggtcccatga aacgagagac tactccgcag aaaataaact
    35941 gtcaattgag cacaagggtt ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg
    36001 tatttgtgag ctgacgcaaa taatctatgg ggatgtaaat aaggtgtgac agaaaaaaaa
    36061 acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat
    36121 gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggatctcttt
    36181 tgcttgccct gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
    36241 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac
    36301 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
    36361 gggcagtgaa aacaccagtg ccagagcaag taagcctttt ttaaacatgt aagcgtcctt
    36421 ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt gcgaaactcc
    36481 cctcaccttt tataacgctg tgccagttta ttagcagccc gacttaacag cggttccagc
    36541 gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc ccagcccgcc
    36601 acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa
    36661 ccaggcttta ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt
    36721 gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta
    36781 aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg ctgctggaag
    36841 gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga
    36901 tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac
    36961 gcaccagttt taccggtaca tcaaaatagt tggcaatccc ggcgcagacg ccgcggacca
    37021 tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc
    37081 cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac gttcgcgcat
    37141 ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact
    37201 gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa
    37261 aatggttaac ggaatagcca gaaatagcgc gcccataagt actccttaga cgatgagcgg
    37321 cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
    37381 aattgtgcca gttgttcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc
    37441 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaatacga
    37501 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga
    37561 cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca tcaatgcctg ctggcgagcg
    37621 cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt tcttcatgcg tgccagcgta
    37681 tcgtccacca gcgtcacttc atgttccagg gacttaatca gatcggtcag tttctgtttt
    37741 tcaattaacg ctgcacgtgc cagatcctct ctctctttca gcagcgccag ttcggctttt
    37801 tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt
    37861 tctgccaacg cgcgcgccga agtagaacgt acttcaacca gtgtatcttc catctcctgg
    37921 atcatcagac gaaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg
    37981 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg
    38041 cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt ttatcttttt
    38101 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgagt tagcgaatta
    38161 cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact tggtgaggcg
    38221 aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg
    38281 ctcatcatcg gcgaacgcgg caccggtaaa gagctgattg ccagccgcct gcattatctc
    38341 tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
    38401 ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat
    38461 ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca
    38521 ccgatgatgg tgcaggagaa attattgcgc gtgattgagt acggtgaact ggagcgcgtt
    38581 ggcggcagcc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc
    38641 ccggcgatgg tcaatgaagg cacttttcgc gctgacctgc tcgaccgact ggcttttgat
    38701 gttgtacaac tgccaccact gcgcgagcgc gaaagcgaca taatgttgat ggcagaacac
    38761 tttgccatcc agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc
    38821 gccagagaaa cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt gaaaaacgtg
    38881 gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga catcattatt
    38941 gatcccttta aacggcgtcc gcctgaagac gctatcgccg tttcagaaac cacctcgctt
    39001 ccaacactgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga gttgctgcaa
    39061 ctcagtttgc aacaagggaa atataaccag aagcgcgcgg ctgaattact ggggttaact
    39121 tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg agcaatacaa
    39181 cttgccggat gcggcgtgaa tgctttattc ggcatacata atcaatacgt cgtttgcagg
    39241 cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct cagcgcatcc
    39301 tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaag
    39361 cgaatcacgt tgccgtatgc gccacaggtc agcaggagca gcccctgcgc cagcgcgcgt
    39421 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
    39481 gccgcaatca tagaccccag accgcgtacc gccgcgatgg ccggaacgct ttctttggca
    39541 tcaatcagcg tgtttgtcag acgctggccc agttgattcg cgcgttcgca gagtgattct
    39601 ttgtcgataa tgttgagcac tgcgtgcgcg gcagccaccg ccagcgggtt accggcgtag
    39661 gtgccgccaa gcccgcccgg cgcgggtgcg tccataatat tcgcgttacc gaccacgccc
    39721 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaaatccgg cttatcggcg
    39781 taatgatcca tggcaaacag cttaccggta cgcgcaaagc cgctttgcac ttcatcagca
    39841 atcatcacaa taccgtgctc gtcgcacagg cggcgaatag cggcaaccag ctcttttggc
    39901 gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa taatcgccgc cacctgcttc
    39961 gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg
    40021 ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg
    40081 attttgtacg gcgcaacttt tccggtcagc gccatggtca tatacgtacg accgtgaaag
    40141 ccgccgctaa acgcaatcac gccagggcgt ccggtatggg cgcgagcaat tttcaccgcg
    40201 ttttccaccg cttccgcacc ggtggtgaag aacgcggttt tggcctgccc gctcaccggg
    40261 gcaagggcgt tgattttctc cgccagcgtg acgtagcttt catacggcac aatctgatac
    40321 gcggtgtggg taaactgttg cagttgctgc tccaccgccg cgaccagatc agggtggcga
    40381 tgtccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt gccctcaaca
    40441 tccttcagcg tggcgttttc agccgactgg gcgaagaagt tacacatcac gccaaccccg
    40501 cgcggagtgg cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
    40561 tgactcttca cggttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaatacgc
    40621 cgcgcccatc gcggtaaacg gcacacgcag cgtgcgcccg ccggggaacg ggtaatgcgg
    40681 cagattggcg aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg caatcaaacg
    40741 tccggctaga tgagtacagg tcacgccgtg gccgctgtag ccctgcatgt aatagatgtt
    40801 ggtatcgagg cgaccaaact gcggcatacg cgacagggtc agcaggaagt tgcccgtcca
    40861 gcggtaatca attttcacgc ccttcagctg cgggaaggtt ttcagcagtt tcggcaccac
    40921 aaggcgctca acgtcatccg ggtcgcgcgc gccgtagacc acgccgccgc cgtacagcag
    40981 gcggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
    41041 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg tgatcacctg
    41101 cgtgccgcac ggcatgctgc gtttcgccag ttccggctct actttatcgc ccagatacgc
    41161 attcccggcg acaatcacat acttcgccgt cacctgacct ttggcagttc gcacaacggc
    41221 tggcgtggtg tgctggatct gcgtcacggc agaaagttca tacacgcgcc cgccgttgag
    41281 gcggatggcg tccgcttcgc caatggcgag gtttagcgga tgaatatgcc caccgctgtg
    41341 atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc
    41401 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt
    41461 ggcgagttgt ttatcattca tcgccacaaa cagaccgccg ggacgatagt cgcagtcaat
    41521 ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
    41581 catacgggcg gtgtccatgc cgtagttttt ttcgatcacg tcgatgtcgc ggctgtagga
    41641 gttcacaagc tgcccgccat tgcgtccgct tgcgccgaag ccgatgcgtg aggcttcaag
    41701 aaccaccacg tcaaagcccg cttccgccag atgcagcgcg gaagagagcc cggtatagcc
    41761 gccgccgacc acgcaaacgt cgcaggaaat cgactcattc agcgtgtcga atggcgcgta
    41821 tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc
    41881 ttatccagat ggttttcagt tcggtgaatt tttcaagggc atgcagggat ttgtcgcgac
    41941 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
    42001 tgacgaagac ggaaccagcc ttcaggcggc ggctcatgcg gtgcgcgcgg gagaggtcgc
    42061 gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt agcgcctggt
    42121 cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
    42181 acgcattcgg gtctacatcc acaaagatgg tcgggccgat ggcggcagcc agctcagcgt
    42241 tacgaccatc cagcagcagt tgccctttgc tttcgccttc ccgaataaag ctatggaccg
    42301 agtcggcgtg ggcgcagtcg attaaagtgc ccatggtggt tgcggggtca agcggatggc
    42361 ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcattg gcgatgctct
    42421 cttccagcaa caggcgtgtt ccggcgatgc acacctgacc ctggttgtag aagatgcctg
    42481 ctgcggtgga gctcgccgct ttttgcagat ccgaacaatc ggcgaaaacg atgttggcgc
    42541 ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct gtcgcccgca tctttcagca
    42601 gctgtttccc ggtacgggtt gaaccggtaa aggcaatggc gtcgatatcg ttatgacgcg
    42661 acagcgcctg ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca
    42721 agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg
    42781 gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca
    42841 acagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca
    42901 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg
    42961 cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg
    43021 gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat
    43081 cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
    43141 gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
    43201 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg
    43261 ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt
    43321 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
    43381 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc ccgtttattt
    43441 ctggcgacat tctttatata cacttcatcc ttcaagccgc ctctgccttg gctgcgttcg
    43501 ctcccccgaa tcacttacct gagtaagctc atcgggattc gctcacttgc cgccttgatg
    43561 caacttgaat gattttgtgt atagattgcc gggcgcattg cgcttacccg gcttacagaa
    43621 caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat acctgccgac
    43681 gtattactga aactgtgcgg tatgccggta ttaatggcat aactttgccc cgcgacgagg
    43741 tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt gcctatttcc
    43801 tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt ttcaaagatc
    43861 atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac accctgactg
    43921 cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg tttttccggc
    43981 tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg cagcgtactg
    44041 atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt cagcccggag
    44101 agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga caagcgtttt
    44161 cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt ggtcagagtc
    44221 gctgtttttc agcgatatgg tgctgacaag cggtgataaa gccctcgaac aatatacgcg
    44281 aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc gcaaaaggat
    44341 gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca acacgcaacc
    44401 gtgggctaac gaccttcgcc ccttgcccat gcagagagtt tacccaaaag ttgctacatt
    44461 caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc gacggtgcat
    44521 attgctgttc caccggcagt tcgggatctt cccgatgttc cagcaattca ggctgttcgc
    44581 acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcctgtaaa ccccggcaga
    44641 tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat taacgccatg ctcagaagat
    44701 cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc ggctgcacat
    44761 tgctgggact accaggaaga taaatgccat cgagtttcgg caaaagttgt tcaagtaatg
    44821 acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg atgatggcat
    44881 tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc ctgcacatta
    44941 cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca aagtggacta
    45001 aattatcgcc attactgcgg tttaaatgac ctttatgttc aatatttttt caatctagca
    45061 gtgggttttc atttttgcaa actcaattta acatttgaca aacatttagt ttgcatacag
    45121 attcgaatgg tggtcattat attttacgct ttgataacga gcggaaaaca aaccaaaggc
    45181 gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag tcagaagaga
    45241 ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac ccgaatacgc
    45301 aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa cgtatccctg
    45361 tttcaagcct gaagaagttg gagaaagggt gttatttccc ggcctcggtg tttgcaatgg
    45421 atattctggg caacgtagta gaagaagcgg gtctgggtca ggaaatgggc gagccggatc
    45481 gtacttgtgt tcctgttctc ggttccttaa ccccttctgc cgccgatcca gagtttatcg
    45541 gtcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt gagccgcgga
    45601 acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg gtcgtagcgg
    45661 tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaagggtat ctgcaaccgc
    45721 cctgcgcgcc cggcaccgat gaccgcaata cacaaagcca ggtttactcc gttgataacc
    45781 tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagtta cagttaattc
    45841 ccgccgacgg tgcggtcgcc gaagcctcgc cgggtcagtt tgaaatcaac ctttaccata
    45901 ccgataacgt gctggaagcc tgcgatgatg cgctggcgct aaaacgcctg gtgcgtctga
    45961 tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa gagcacgcgg
    46021 gcagcggaat gcatatccat atcagtatgc aaaataaccg tggcgagaac gtgctttctg
    46081 acgcggaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg attgacctga
    46141 tgccgtcatc aatggcgttg ctggcaccaa acgtgaactc gtatcgccgc ttccagccgg
    46201 gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc gccctgcgta
    46261 ttccgtgcgg cgaccgtcat aatcaccgcg tggaatatcg cgtggcgggt gccgatgcca
    46321 acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt gataacgagc
    46381 tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta cccttcccga
    46441 ttcgccagag cgatgccctg ggtgagttta tcgagaatga tcacctgcgc cgctatttag
    46501 gcgaacggtt ttgccatgtg tatcacgcct gtaaaaatga tgagctgttg cagtttgagc
    46561 gcctcatcac agaaacagaa attgagtgga tgttgaaaaa cgcgtaattc cccttgcggg
    46621 gttgtttcat cggcctgagc ggagccaggc ctgacatttg ctggtaatcc agacatttcc
    46681 cggatgtcgc gttatggagc gcaggcggca acgggcttcc gttaacgatt aagactgtga
    46741 cgaggaaatg agatgatgcc gatgattaaa tccccgcatg gcgagggagg ctgcgtatgc
    46801 gcccctcctg ctactgactg gactccgccc cctttgcttc ccttgctaaa caggtttgat
    46861 ttcaggtcga cacgaccgca aaccttatta cgcaggggag gcagcaatta tggctattaa
    46921 ttcaccactg aatattgctg cgcaacccgg caaaacccgt ctgcgaaaat cactgaaatt
    46981 gtggcaggtg gtaatgatgg gtctggccta tctcacgccg atgactgtgt tcgatacttt
    47041 tggcattgtg tcaggcatta gcgacggtca cgttccggcg tcctatttgc tggcgctggc
    47101 gggcgtgctg tttaccgcta tcagctacgg caaactggtt cgccagtttc cggaggccgg
    47161 ttcggcctat acctacgcgc aaaagtcgat taacccgcac gtcggattta tggtcggctg
    47221 gtcatcactg ctggattatc tctttttgcc gatgatcaac gttttgttgg cgaaaatcta
    47281 tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg gtggtaacct tcgtcgccat
    47341 tttaaccgcc gcgaatctga agagcgtcaa cctggtcgct aacttcaata ccctgtttgt
    47401 gttggtgcaa atctccatca tggtggtgtt tatcttcctg gtggttcagg gactgcataa
    47461 aggagaaggc gttggcacgg tctggtcgtt acagccgttt atcagcgaga acgcgcatct
    47521 gattccgatt attaccgggg cgacgatcgt ctgcttctct ttccttggtt tcgatgcagt
    47581 gaccacactt tcggaagaga caccagacgc cgcacgcgtg atcccgaaag ccatcttcct
    47641 gacggcggtc tatggtggcg ttatctttat cgcggcgtcg ttctttatgc agctgttctt
    47701 ccccgatatc agccgcttta aagacccgga cgccgcactg cctgaaattg cactctacgt
    47761 cggcggtaag ctgttccagt cgattttcct ttgcaccacg tttgtgaaca cgttagcgtc
    47821 tggcctggcc tcacatgcca gcgtgtcacg tctgctgtat gtgatggggc gcgacaatgt
    47881 gtttccggag cgcgtgtttg gctatgtgca tccgaaatgg cggactccgg cactgaacgt
    47941 cattatggtc gggattgtcg cgatgtcggc gctgttcttc gatttagtta ccgccacagc
    48001 gttaattaac ttcggcgcgt tggtagcgtt taccttcgtt aacctgtcgg tgttcaacca
    48061 cttctggcgg cgcaaaggaa tgaataaaag ctggaaggaa cacttccact atttattaat
    48121 gccgctggtt ggcgcgctga cggtgggcgt gctgtggatt aacctcgaag ccacctcgct
    48181 gacgctgggt ctggtatggg cttcgttagg cggcgcatat ttgtggtatc tgattcgccg
    48241 ctatcgcaaa gtgccgctgt acgaaggtga cagaacgcca gtgagcgaaa cgtaatagtc
    48301 ggatgcgcgc cgcattatcc tgcctgaggg taatgcgccg cgccattaac tataaaaaga
    48361 tgtaagaccc ggatcacgag tgctatcatc cgcccttatg cttagcgcat taaccccagg
    48421 ggcaaataat gaaccacgat atcccgctaa aatattttga tattgccgat gagtacgcga
    48481 ccgaatgcgc agagcctgtc gcagacgctg aacgcacacc gctggcccac tacttccagt
    48541 tactgctcac ccgtttgatg aacaatgaag agatcagcga agaagcccaa catgaaatgg
    48601 ctgctgaagc ggggattaat cccgtacgca ttgatgagat cgcagagttt ttgaatcaat
    48661 ggggtaatga gtaattattt aattgcagac gatcggtcag tatcgttgtt cggataaagc
    48721 gttcacgccg catccggcta acaatgacac gcactgcagc gtgctttaat gggtttctca
    48781 cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag ttatagttat agagaaatca
    48841 cgggtacgaa tgttgaacag atggcgacgc cacctgttca acgaccaacc acacggccct
    48901 cattacgacc cctgaacaca aacggtaatg gcaaatgtca ttaaatatta gtaagatagc
    48961 ctctttacat aaaatccctt aatattatgc gccaggtatt atcgtctctt ttggtgattg
    49021 ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc ccccccgcat gctgatatcc
    49081 gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa cacctttaac ccatccaaag
    49141 cgagcagtgg gttaattgtc gatacccttg ccgcccagtt ttatgatcga ctgctggatg
    49201 tcgatcccta tacctatcgc ctgatgccgg aacttgccga aagctgggaa gtactcgaca
    49261 acggcgcgac ctatcgcttc cacctgcgtc gcgatgttcc gtttcaaaaa accgactggt
    49321 ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt taccttccag cgaatttttg
    49381 accgcaacaa cccgtggcat aacgtcaacg gcagcaactt cccctacttc gacagcctgc
    49441 aatttgccga taacgtgaaa agcgtccgca aactggataa tcataccgtt gagttccgac
    49501 tcgcccagcc ggatgcttct tttttgtggc acctcgcaac ccattatgct tcggtcatgt
    49561 cggcagaata tgcccggaag ttagagaaag aagatcgcca ggagcaactc gaccgtcaac
    49621 cggtcggcac cggtccgtat cagttgtcgg aataccgcgc cgggcaattt attcgcctac
    49681 aacgtcatga tgacttctgg cgcggtaaac cgttaatgcc gcaggtagtg gtggatttag
    49741 gctccggcgg caccggacgt ctgtcgaaac tcctgaccgg ggaatgcgac gttctggcct
    49801 ggcctgctgc cagccagcta tccattttgc gtgacgaccc gcgcttgcgt ttaacgctgc
    49861 gtcctgggat gaacgtcgcc tatctggcat ttaacaccgc caaaccgccg ctaaataatc
    49921 ccgctgtccg ccatgcactg gcactggcga ttaataacca gcgcctgatg caatccatct
    49981 attatggtac ggctgaaacg gcggcctcta ttttaccgcg cgcctcgtgg gcctatgaca
    50041 acgaggctaa aattactgaa tacaatccgg cgaaatcgcg cgaacagttg aaggcgttgg
    50101 ggctggaaaa cttaacgctg aaactgtggg tgcccacacg ttcgcaggcg tggaacccca
    50161 gtccactgaa aactgccgaa ctgattcagg cggatatggc gcaggttggc gtaaaagtgg
    50221 tgattgtgcc ggtagaaggt cgctttcagg aggcgcggtt gatggatatg agccatgatc
    50281 tgacgttatc cggttgggcg acggacagta acgacccgga cagtttcttc cgtccgttac
    50341 tgagctgcgc ggcaattcat tcacagacca acctcgccca ctggtgcgat ccgaaattcg
    50401 acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc agcgcgtatt gaagcctatg
    50461 acgaagcgca gagtattctg gcgcaggaat tacctattct gccgctggcg tcgtcattgc
    50521 gtttgcaggc ctatcggtac gatatcaaag gtctggtact tagcccgttt ggtaacgcct
    50581 cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa aaaaccatga ttatcttcac
    50641 cttacgccgc attttgttat tgattgtcac cttgttcctg ctgaccttcg ttggcttcag
    50701 tttgagctat ttcactccac acgcgccgtt acaaggcgcg tcactgtgga atgcctgggt
    50761 gttctggttt aacggcctga tccactggga ttttggtgtt tccagcatta acggtcaacc
    50821 gattgccgaa cagttaaaag aggtcttccc tgcgacgatg gaactgtgca tcctcgcctt
    50881 cggttttgcg ctgattgttg gcatccccgt ggggatgatt gccgggatta cgcgccataa
    50941 gtggcaggat aacttgatca acgccatcgc cttgctgggc ttttcaatcc cggtgttctg
    51001 gctggcgctt ctgttgacgc tgttttgttc actcacgcta ggttggttgc ccgtttcagg
    51061 gcgtttcgat ctgctgtacg aagtgaaacc gattaccggg tttgcgttga ttgatgcctg
    51121 gctttcgtac tcaccatggc gggatgaaat gatcatgagc gcaatccgcc atatgatatt
    51181 gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg atccgactga tgcgtatcag
    51241 cactatcgaa gtgtatgacc agaactatgt taaagcggcg gctacccgcg gtttgtcgcg
    51301 ctttactatt ttgcgtcgcc acgttctgca taacgcgtta ccgccggtta ttcctcgcct
    51361 gggcttacag ttttccacca tgctgacgct ggcaatgatc accgaaatgg tttttagctg
    51421 gccgggttta gggcgctggt taattaacgc catccgccag caggattacg cagccatttc
    51481 cgccggagtg atggtgtgtg gctcactggt cattattgtt aacgtgattt ctgatatttt
    51541 gggtgccatg gctaaccctc tgaaacataa ggaatggtat gccttacgat agcgtataca
    51601 gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg caaattttat agtgacgcct
    51661 ctgcgatggt cggcctgtac ggctgcgcgg ggctggctgt actgtgtatt tttggcggct
    51721 ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta ccaattattg ccgccgtcat
    51781 ggtcgcgcta tggcgaagtt tctttcttcc tggggactga cgacctcgga cgcgatgttt
    51841 taagccgttt actgagcgga gctgcgccaa ccgtgggtgg cgcatttgtg gtgacgcttg
    51901 ccgcgacgat ctgtggcctg gtactgggca ccttcgccgg ggcgacgcac ggcctacgct
    51961 cagcggtgct taaccatatt ctggatacct tactggcgat cccttcgctg ctgctggcaa
    52021 ttatcgttgt agcgtttgcc ggaccgagtt tgtcccacgc tatgtttgct gtctggctgg
    52081 cattgctgcc gcgtatggta cgttcgattt acagcatggt gcatgacgaa ctggaaaaag
    52141 agtacgttat cgccgcccgt ctggatggcg catcaacgct gaatattctc tggtttgctg
    52201 tgatgccaaa catcaccgcc gggctggtca ccgagatcac ccgagcactg tcgatggcaa
    52261 ttctcgatat cgccgcgctg ggctttctcg atctcggcgc acaactcccc tcgcctgaat
    52321 ggggagcgat gctcggtgat gcgctggaac tgatttatgt cgccccgtgg actgtcatgc
    52381 tgccaggtgc ggcaattatg attagcgtgt tgttggttaa cctccttggc gatggcgttc
    52441 gtcgtgcgat tattgcgggg gtggaataat gccattactc gatattcgta acctgaccat
    52501 tgaatttaaa accggtgatg agtgggttaa agccgtcgac cgggtaagta tgaccttaac
    52561 cgaaggtgaa atccgcggtc ttgttggtga atccggttcc ggcaagagtt tgattgcgaa
    52621 agcgatttgt ggggtgaata aagataactg gcgtgttact gctgaccgta tgcgttttga
    52681 tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc aaactggttg gccataacgt
    52741 gtcgatgatt ttccaggaac cgcagtcgtg tcttgaccct tcagaacgtg tgggtcgcca
    52801 gttgatgcaa aacatcccag cctggaccta taaaggccgt tggtggcagc gttttggctg
    52861 gcgcaaacgc cgtgccattg aactgctgca ccgcgtgggg attaaagatc acaaagatgc
    52921 gatgcgtagt tttccctatg agttgaccga aggtgaatgt cagaaagtga tgatagccat
    52981 tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa ccgaccaact caatggagcc
    53041 aacaacccag gcgcaaatct ttcgcctgct gacgcgtctc aaccaaaaca gcaataccac
    53101 tattttgctt atcagccatg acttacaaat gcttagccaa tgggcggata aaattaacgt
    53161 gctttactgc ggtcaaacag tggaaaccgc gccgagtaag gagttggtga cgatgccaca
    53221 tcatccttat acccaggcgc tgatccgcgc gataccagac ttcggcagcg cgatgccgca
    53281 taaaagtcgc ctcaatacgc tgcccggcgc tatcccactg ctggaacagt taccgattgg
    53341 ttgtcgtctg gggccacgtt gcccgtatgc acaacgagaa tgcattgtga cgccacgttt
    53401 gacgggggcg aaaaatcatc tctatgcctg tcatttcccg ctgaacatgg agaaagagtg
    53461 agatgatcga aacgctgctt gaagtgcgta atttaagtaa aacgttccgc taccggaccg
    53521 gctggtttcg tcgtcagacc gtagaagcgg taaaaccctt gagctttacg ctacgtgaag
    53581 gccagacact ggcgattatt ggcgagaatg gttcgggtaa atccacgctg gcaaaaatgc
    53641 tggcgggaat gatagagcct accagcggcg agttattgat tgacgatcat ccactgcatt
    53701 ttggcgatta ttccttccgc agtcagcgta ttcgcatgat ttttcaggat ccttcgacct
    53761 cgttgaatcc ccgtcaacgt atctcgcaaa ttctggattt tccactgcgc ctgaacaccg
    53821 acctggaacc ggaacagcgt cgcaaacaga ttattgaaac gatgcgtatg gttgggctgc
    53881 taccggatca cgtcagttac tatccgcata tgttggcacc cggacaaaaa cagcgtctgg
    53941 gtctggcgcg cgcgctgata ttgcgcccaa aagtcattat tgccgatgaa gccctcgctt
    54001 cactggatat gtcgatgcgt tcgcagttga ttaatctgat gctggaatta caggaaaaac
    54061 agggcatttc gtatatttat gttacccagc atatcggaat gatgaagcac attagcgacc
    54121 aggtgctggt gatgcatcag ggcgaggttg tcgaacgagg gagcaccgcg gatgtgctgg
    54181 catcgccgct gcatgaactc accaaacggc tgattgccgg gcactttggc gaagcattga
    54241 cagcggatgc gtggcgtaaa gatcgctaat tttctgaatc ctccttccag attatccacc
    54301 tcattctgac aagagattaa atcttgtcag aatgaggtga tggataaaat aaaatcaaat
    54361 gcgcgtgatt taagacgcaa tctcactttg caggaacgaa agctctggcg atatcttcgc
    54421 agccgacgtt ttggtgattt caaatttcgc cgtcaacatc cggtggggag ctacattctc
    54481 gattttgctt gctgctcggc gcgcgtagtc gttgagctgg atggtgggca gcatgatttg
    54541 gcagttgcct atgataccag gcgcactagc tggcttgagt cgcagagctg gaccgtgctg
    54601 cgtttctggc ataacgagat tgattgtaat gaggaggcgg tgctggagat tattctgcag
    54661 gaactgaacc gccggtcacc ctcaccctga aagggcgagg gggccgaccg agccgaatag
    54721 ctgttgtggt gaaaacatgg agacagtgct ggagaatatt cggcaaggtc tgaaccatcc
    54781 cagccatcgc cataaaaggg ttaggggctg tatgagcctg tttgttgctg gggtaacaat
    54841 atttgcacaa tatggtcccc tcgcccctct ggggagaggg ttagggtaag gggaaaagcg
    54901 cccaccctgc cgcagcctgc tccggtcgga cctggcaact atagctactc acagccaggt
    54961 tgattataat aaccgtttat ctgttcgtac tgtttactaa aacgacgaat cgcctgattt
    55021 tcaggcacaa caagcatcaa caataaggat taaagctatg ggttttcttt ccggtaagcg
    55081 cattctggta accggtgttg ccagcaaact atccatcgcc tacggtatcg ctcaggcgat
    55141 gcaccgcgaa ggagctgaac tggcattcac ctaccagaac gacaaactga aaggccgcgt
    55201 agaagaattt gccgctcaat tgggttctga catcgttctg cagtgcgatg ttgcagaaga
    55261 tgccagcatc gacaccatgt tcgctgaact ggggaaagtt tggccgaaat ttgacggttt
    55321 cgtacactct attggttttg cacctggcga tcagctggat ggtgactatg ttaacgccgt
    55381 tacccgtgaa ggcttcaaaa ttgcccacga catcagctcc tacagcttcg ttgcaatggc
    55441 aaaagcttgc cgctccatgc tgaatccggg ttctgccctg ctgacccttt cctaccttgg
    55501 cgctgagcgc gctatcccga actacaacgt tatgggtctg gcaaaagcgt ctctggaagc
    55561 gaacgtgcgc tatatggcga acgcgatggg tccggaaggt gtgcgtgtta acgccatctc
    55621 tgctggtccg atccgtactc tggcggcctc cggtatcaaa gacttccgca aaatgctggc
    55681 tcattgcgaa gccgttaccc cgattcgccg taccgttact attgaagatg tgggtaactc
    55741 cgcggcattc ctgtgctccg atctctctgc cggtatctcc ggtgaagtgg tccacgttga
    55801 cggcggtttc agcatcgctg caatgaacga actcgaattg aaataatcat tctgttggta
    55861 aatattgggc ggcgttctgc cgcccgttat ctctgttata cctttctgat atttgttatc
    55921 gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat aacgatttcc tttacgacca
    55981 aggagcgccc atggaacaac gccacatcac cggcaaaagc cactggtatc atgaaacgca
    56041 atccagtact gcggagtatg acgttctgcc tctggtcccg gaagccgcaa aggtcagcga
    56101 tccctttctg ctcgacgtga tccttgatga agaaacgctg gttcccttcc tttcatggct
    56161 ggtccctgcg cgcgttcttg cagtggaatt gttccctgac cagcttaccg tgacccgttc
    56221 acagaccttc accgcttatg aacgcttgtc tacggccctg acggttgctc aggtttgcgg
    56281 cgtccagcgg ttatgtaact actattcggc gcgacttacg ccgctccccg ggcctgattc
    56341 ctccagggaa agcaatcata ggttggcaca aatcacgcaa tatgcccgcc aactggctag
    56401 ctcgccttct attatcgaca accgatcgcg ccagcatctg aatgacgtcg gtcttactgc
    56461 cggggactgt gtgatcatta accaaatcat tggttttatt ggctttcagg cgcggaccat
    56521 tgcgacattt caggcttatc tcgggcaccc ggtacgctgg ttacccggtc tggaaataca
    56581 aaactacgcc gacgcgtcac tgtttgctga tgaatcaata cgctggcgaa gcagctatga
    56641 ggtggaaaaa ctacctgaag agtacacaaa aagttcaaca gcagaacttt gccaactggc
    56701 cgaaacactc tctctccacc ctatttcact ttcccttctc gaaaggttgt taaacagtac
    56761 acgggttaat acacagccgg ataataagct tgcggcgttg ttatgcgcgc ggataaatgg
    56821 cagtcctgct tgttttgccg cctgtatgga ttcatcaaat gaatataaaa aaatcagcgc
    56881 ccttgtgcgc aagggcgaaa atgaaattaa ccaatgggct gaccgtcatt ctgttgagca
    56941 cgctaccgtt caggcgatac aatggctgac ccgagcaccc gatcgcttta gcgccgccca
    57001 gttcagccct ttactcgaac acgaaaaatc atcaacgcag attattaatc tgctggtatg
    57061 gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg ttgggtgaga catattaacc
    57121 ttgccgcgtc agacagattc gcgtaaaact gtcagccgct ctaatggcca ccaaaataga
    57181 caattatgtt tcaggacaac ccgctgctag cgcagcttaa acagcaactg cattcccaga
    57241 cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg ctttggcttc ctggaagtcg
    57301 acgcgcaaaa aagttatttc attccgccgc cgcagatgaa aaaagtcatg catggcgacc
    57361 gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc cgcagagcca gaagaactgg
    57421 ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg caaaaatgac cgtctggcca
    57481 tcgttcctga tcatccactc ttaaaagacg ccattccttg ccgcgcagcc cgtggcctga
    57541 accacgagtt taaagaaggc gactgggcgg ttgccgaaat gcgccgtcat ccgctgaaag
    57601 gcgatcgttc tttctatgct gaactgacac aatacatcac ttttggtgac gaccactttg
    57661 taccgtggtg ggttacactt gcgcgccata atctggaaaa agaagcacca gacggcgtcg
    57721 ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct gaccgcgctg gattttgtca
    57781 ccatcgacag tgccagcaca gaagatatgg atgacgccct tttcgctaag gcgttgccgg
    57841 atgacaaact tcagctgatt gtggcgattg ccgatccaac cgcgtggatt gcagaaggca
    57901 gcaagctgga caaagccgcg aaaattcgcg cattcaccaa ctatctgcct ggcttcaaca
    57961 tccctatgct gcctcgcgag ctttctgacg atctctgctc actgcgcgcc aatgaagtcc
    58021 gcccggtact ggcatgccgc atgacgctct ccgctgatgg caccattgaa gataatatcg
    58081 aattctttgc cgccaccatc gaatccaaag cgaagctggt gtatgaccag gtttctgact
    58141 ggctggaaaa taccggtgac tggcagcctg aaagtgaagc aattgccgaa caagtccgtt
    58201 tgttagcgca aatttgccaa cgccgcggcg agtggcgtca taaccacgca ctggtgttta
    58261 aagatcgccc ggattaccgc tttattctcg gtgaaaaagg tgaagtgctg gatatcgtcg
    58321 ccgagccccg tcgcattgcc aaccgtatcg tcgaagaagc gatgattgcc gctaacattt
    58381 gtgcggcccg cgtactgcgc gataagctcg gttttggcat ctataacgtg catatgggct
    58441 ttgatccggc aaatgccgac gcgctggcag cgttgctgaa aacgcacggt ctgcatgtcg
    58501 atgccgaaga agtgctcacg ctggacggtt tctgcaaact gcgtcgtgaa ctggacgcgc
    58561 aaccaactgg tttcctcgac agccgcattc gtcgcttcca gtcatttgct gaaattagca
    58621 ctgaacccgg tcctcacttt ggcctcggtc tggaagcata cgccacctgg acttcgccga
    58681 tccgtaaata tggcgacatg atcaaccacc gtctgctgaa agcggttatc aaaggcgaaa
    58741 ctgcgacgcg cccacaggat gaaatcactg tccaaatggc cgagcgtcgc cgtctcaacc
    58801 ggatggcaga acgtgatgtt ggcgactggt tatacgcacg cttcctgaaa gacaaagccg
    58861 ggaccgacac ccgtttcgcg gcggaaattg tcgatatcag ccgtggcggc atgcgtgtgc
    58921 gtttggttga taacggcgct atcgccttta ttccggcacc tttcttacac gctgtgcgcg
    58981 atgaactggt ttgcagccag gaaaacggca ctgtacaaat taaaggtgaa acggtttaca
    59041 aagtaactga cgttattgac gtcaccattg ccgaagtccg catggaaacc cgcagcatta
    59101 ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc ctcatggatg ggccgtttat
    59161 ttccccgctc taccttcgtt atatccaggc agatttttta actgactttc gtttgaaaac
    59221 tggcgttttt ccaaaactgg attacaaata aatatataaa aaaaacaatg cgtttagccg
    59281 ccgttaattt catgtaagtc atgttgttcc ttttatttaa ccgcggactc cgcagttaac
    59341 cggaggatat gcatcatgaa aaccgttagg gagtccacaa cgttgtacaa ctttctcgga
    59401 tcgcacaatc catactggcg gttgacggaa agcag
//
LOCUS       DAMZII010000013        59370 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZII010000013 DAMZII010000000
VERSION     DAMZII010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.013"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            42..758
                     /locus_tag="POA77_000774"
     CDS             42..758
                     /locus_tag="POA77_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6364252.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            788..1288
                     /locus_tag="POA77_000775"
     CDS             788..1288
                     /locus_tag="POA77_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6364253.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1380..4064
                     /locus_tag="POA77_000776"
     CDS             1380..4064
                     /locus_tag="POA77_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDI6364254.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4061..5149
                     /locus_tag="POA77_000777"
     CDS             4061..5149
                     /locus_tag="POA77_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDI6364255.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5237..6535)
                     /gene="exuT"
                     /locus_tag="POA77_000778"
     CDS             complement(5237..6535)
                     /gene="exuT"
                     /locus_tag="POA77_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDI6364256.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7018..8430
                     /gene="uxaC"
                     /locus_tag="POA77_000779"
     CDS             7018..8430
                     /gene="uxaC"
                     /locus_tag="POA77_000779"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDI6364257.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8445..9932
                     /gene="uxaA"
                     /locus_tag="POA77_000780"
     CDS             8445..9932
                     /gene="uxaA"
                     /locus_tag="POA77_000780"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDI6364258.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10015..10566
                     /locus_tag="POA77_000781"
     CDS             10015..10566
                     /locus_tag="POA77_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDI6364259.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10571..11815)
                     /gene="sstT"
                     /locus_tag="POA77_000782"
     CDS             complement(10571..11815)
                     /gene="sstT"
                     /locus_tag="POA77_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDI6364260.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12431..13396)
                     /gene="alx"
                     /locus_tag="POA77_000783"
     CDS             complement(12431..13396)
                     /gene="alx"
                     /locus_tag="POA77_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDI6364261.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13679..14665)
                     /locus_tag="POA77_000784"
     CDS             complement(13679..14665)
                     /locus_tag="POA77_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6364262.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14744..15427)
                     /locus_tag="POA77_000785"
     CDS             complement(14744..15427)
                     /locus_tag="POA77_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDI6364263.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15504..16007)
                     /locus_tag="POA77_000786"
     CDS             complement(15504..16007)
                     /locus_tag="POA77_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDI6364264.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16092..17228
                     /gene="rlmG"
                     /locus_tag="POA77_000787"
     CDS             16092..17228
                     /gene="rlmG"
                     /locus_tag="POA77_000787"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDI6364265.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17419..17502
                     /locus_tag="POA77_000788"
                     /pseudo
     CDS             17419..17502
                     /locus_tag="POA77_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17499..17915
                     /gene="higA"
                     /locus_tag="POA77_000789"
     CDS             17499..17915
                     /gene="higA"
                     /locus_tag="POA77_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDI6364266.1"
                     /translation="MIALSNILQAGEQLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMVEAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVSSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17960..19978)
                     /gene="fadH"
                     /locus_tag="POA77_000790"
     CDS             complement(17960..19978)
                     /gene="fadH"
                     /locus_tag="POA77_000790"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDI6364267.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20304..22655)
                     /gene="ygjK"
                     /locus_tag="POA77_000791"
     CDS             complement(20304..22655)
                     /gene="ygjK"
                     /locus_tag="POA77_000791"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI6364268.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFMVKKSDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22672..23742)
                     /gene="ygjJ"
                     /locus_tag="POA77_000792"
     CDS             complement(22672..23742)
                     /gene="ygjJ"
                     /locus_tag="POA77_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDI6364269.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23876..25309)
                     /locus_tag="POA77_000793"
     CDS             complement(23876..25309)
                     /locus_tag="POA77_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6364270.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25372..25821)
                     /locus_tag="POA77_000794"
     CDS             complement(25372..25821)
                     /locus_tag="POA77_000794"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDI6364271.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25818..28910)
                     /gene="ebgA"
                     /locus_tag="POA77_000795"
     CDS             complement(25818..28910)
                     /gene="ebgA"
                     /locus_tag="POA77_000795"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDI6364272.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29094..30077)
                     /gene="ebgR"
                     /locus_tag="POA77_000796"
     CDS             complement(29094..30077)
                     /gene="ebgR"
                     /locus_tag="POA77_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDI6364273.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30296..30628
                     /locus_tag="POA77_000797"
     CDS             30296..30628
                     /locus_tag="POA77_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDI6364274.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30670..32049)
                     /gene="ygjG"
                     /locus_tag="POA77_000798"
     CDS             complement(30670..32049)
                     /gene="ygjG"
                     /locus_tag="POA77_000798"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDI6364275.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32467..33987
                     /gene="aer"
                     /locus_tag="POA77_000799"
     CDS             32467..33987
                     /gene="aer"
                     /locus_tag="POA77_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDI6364276.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDASSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(34141..34764)
                     /gene="nfeR"
                     /locus_tag="POA77_000800"
                     /gene_synonym="yqjI"
     CDS             complement(34141..34764)
                     /gene="nfeR"
                     /locus_tag="POA77_000800"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDI6364277.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            35041..35805
                     /gene="nfeF"
                     /locus_tag="POA77_000801"
     CDS             35041..35805
                     /gene="nfeF"
                     /locus_tag="POA77_000801"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDI6364278.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35859..35934)
                     /locus_tag="POA77_000802"
     tRNA            complement(35859..35934)
                     /locus_tag="POA77_000802"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35899..35901),aa:Ile,seq:cat)
     gene            36059..36565
                     /gene="mug"
                     /locus_tag="POA77_000803"
     CDS             36059..36565
                     /gene="mug"
                     /locus_tag="POA77_000803"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDI6364279.1"
                     /translation="MVEDILAPGLRVVFCGINSGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36644..38485)
                     /gene="rpoD"
                     /locus_tag="POA77_000804"
     CDS             complement(36644..38485)
                     /gene="rpoD"
                     /locus_tag="POA77_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI6364280.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38680..40425)
                     /gene="dnaG"
                     /locus_tag="POA77_000805"
     CDS             complement(38680..40425)
                     /gene="dnaG"
                     /locus_tag="POA77_000805"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6364281.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40536..40751)
                     /gene="rpsU"
                     /locus_tag="POA77_000806"
     CDS             complement(40536..40751)
                     /gene="rpsU"
                     /locus_tag="POA77_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI6364282.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40989..42002
                     /gene="tsaD"
                     /locus_tag="POA77_000807"
     CDS             40989..42002
                     /gene="tsaD"
                     /locus_tag="POA77_000807"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI6364283.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(42045..43508)
                     /gene="ttdT"
                     /locus_tag="POA77_000808"
     CDS             complement(42045..43508)
                     /gene="ttdT"
                     /locus_tag="POA77_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDI6364284.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43557..44162)
                     /gene="ttdB"
                     /locus_tag="POA77_000809"
     CDS             complement(43557..44162)
                     /gene="ttdB"
                     /locus_tag="POA77_000809"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDI6364285.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(44159..45070)
                     /gene="ttdA"
                     /locus_tag="POA77_000810"
     CDS             complement(44159..45070)
                     /gene="ttdA"
                     /locus_tag="POA77_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDI6364286.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45277..46209
                     /gene="ttdR"
                     /locus_tag="POA77_000811"
                     /gene_synonym="dan"
     CDS             45277..46209
                     /gene="ttdR"
                     /locus_tag="POA77_000811"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDI6364287.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46222..46839)
                     /gene="plsY"
                     /locus_tag="POA77_000812"
     CDS             complement(46222..46839)
                     /gene="plsY"
                     /locus_tag="POA77_000812"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI6364288.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46944..47312
                     /gene="folB"
                     /locus_tag="POA77_000813"
     CDS             46944..47312
                     /gene="folB"
                     /locus_tag="POA77_000813"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDI6364289.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47402..48223
                     /gene="bacA"
                     /locus_tag="POA77_000814"
     CDS             47402..48223
                     /gene="bacA"
                     /locus_tag="POA77_000814"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6364290.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48386..49624)
                     /gene="cca"
                     /locus_tag="POA77_000815"
     CDS             complement(48386..49624)
                     /gene="cca"
                     /locus_tag="POA77_000815"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDI6364291.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
     gene            complement(49688..50308)
                     /locus_tag="POA77_000816"
     CDS             complement(49688..50308)
                     /locus_tag="POA77_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDI6364292.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50550..51851
                     /gene="ygiF"
                     /locus_tag="POA77_000817"
     CDS             50550..51851
                     /gene="ygiF"
                     /locus_tag="POA77_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDI6364293.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51874..54714
                     /gene="glnE"
                     /locus_tag="POA77_000818"
     CDS             51874..54714
                     /gene="glnE"
                     /locus_tag="POA77_000818"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDI6364294.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSHSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEA"
     gene            54762..56195
                     /gene="hldE"
                     /locus_tag="POA77_000819"
     CDS             54762..56195
                     /gene="hldE"
                     /locus_tag="POA77_000819"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDI6364295.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56315..56374)
                     /gene="ibsE"
                     /locus_tag="POA77_000820"
     CDS             complement(56315..56374)
                     /gene="ibsE"
                     /locus_tag="POA77_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDI6364296.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56691..56750)
                     /locus_tag="POA77_000821"
     CDS             complement(56691..56750)
                     /locus_tag="POA77_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6364297.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(56989..58650)
                     /locus_tag="POA77_000822"
     CDS             complement(56989..58650)
                     /locus_tag="POA77_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDI6364298.1"
                     /translation="MDDIVNSVSSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(58677..59306)
                     /locus_tag="POA77_000823"
     CDS             complement(58677..59306)
                     /locus_tag="POA77_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDI6364299.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
BASE COUNT        14242 a        15155 c        15786 g        14187 t
ORIGIN      
        1 acaccaactt tttggaaaca atattgtaag atcaaattta tatggtggcg aaaattaatc
       61 tcttatcacg gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata
      121 tgactgttta tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa
      181 tatctaaaag taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat
      241 cgacgcctca ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta
      301 tgccacctaa attcgcttta ccagccggta gtagcaaaac cgtacgtttt gttgcgatgg
      361 aaccagagca aaaagagaaa aattatcgcg ttaaatttga agcggttccc agtattgatg
      421 acgttgccac agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga
      481 ttgttgttag tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa
      541 aattagttaa tgcagggaat caacgcttaa agattttaac gatcgcttat tgtaaaaata
      601 atagcaaaga aaattgtaag atacagaccg taaataaaaa catcttccct ggtcaggaaa
      661 gaaatcttga aagcatatct ggctacgaca aaatcgttgt caaatataat aactggatca
      721 ccaaagataa tggcgagttt gaactggcgg tccattaatt atcattgtaa taaacgaagg
      781 aatagtaatg aaaaaggtat ttgcaaaatc tcttctggtc gcagcgatgt tttctgttgc
      841 aggctccgca ttggcagtac aaaaggatat tactgtaacg gccaacgttg atgccgctct
      901 ggatatgaca cagaccgata acaccgcgtt gccgaaagca gtagaaatgc aatatctgcc
      961 ggggcagggt ctgcaatctt accagttgat gaccaaaatc tggtctaacg acgttaccaa
     1021 agatgtaaaa atgcagttag tgtccccagc gcaactggtt caaagcctgg atgctagcaa
     1081 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
     1141 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
     1201 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
     1261 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
     1321 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
     1381 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
     1441 taaccaaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
     1501 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
     1561 catttcttga tccagcaagt ctatataaca agctggagct ggatgtagac gatcagaaaa
     1621 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcgccacggt gaattggctt
     1681 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
     1741 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
     1801 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
     1861 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
     1921 gtgcgctggg aataacagaa aatagctata ttggtgcaca ctggaatttc aacggttatg
     1981 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
     2041 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     2101 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     2161 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     2221 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     2281 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgcctggc agctacagcg
     2341 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     2401 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     2461 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     2521 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     2581 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     2641 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     2701 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     2761 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     2821 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     2881 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     2941 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     3001 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     3061 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     3121 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     3181 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     3241 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     3301 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     3361 cggcctttac gggcacttat agctctacgc tggctgtcag tcgctatggc gttaatttgg
     3421 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     3481 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     3541 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     3601 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     3661 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     3721 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     3781 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggcggattt acacttgaaa
     3841 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     3901 taagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     3961 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     4021 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     4081 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     4141 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     4201 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     4261 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     4321 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     4381 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     4441 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     4501 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     4561 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     4621 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     4681 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     4741 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     4801 agatgaaggg cgaccagctg cagggcgcag agacggttac ttctctatct atcgtcaggg
     4861 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     4921 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     4981 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     5041 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     5101 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattaac cctgcaaagc
     5161 ggacaaacgc ttttttctat ataaacgacg aagccgtctg ataaagcggc ttcgtcatta
     5221 caacttaagt taaacgttaa tgttgcggtg cgggatcgtt atgtgtttcc tgcgccacct
     5281 caatcgccgg tttgttctgc aacacggtcc agataaccag cgcacctaac aggtcaaata
     5341 ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc agccagtgca ccgacaacca
     5401 gcgcaaacag cgtacttgcc agccatgcgg acatcccggt taaaccgttc gccgtcgcca
     5461 cttcgttacg accgaacacg tcagaagaga gcgtaatcag cgcgccagac agtgcctggt
     5521 gggcaaaacc accgatacac agcagcataa ttgcgacata cgggttggtg aacagaccga
     5581 tcatacccgg gccaatcatc agcacagcac ccagcgttac gaccatctta cgggaaacga
     5641 tcaggttcac accaaaccaa cgctggaaca gcggcggcag gtaaccaccg aggatacaac
     5701 cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc gatctctttc aggttaaagc
     5761 cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa agtaccccag gccggttctg
     5821 ccaggaaacg cggcagcgcg ataccccaga actgacggtt acgcaggatc tgaccaacgg
     5881 acattttctt cgccgtgctc acctggtgct gggcttcctg accattaata atatagtcgc
     5941 gttcttcatc ggtcagatgc ttctggtcgc gtggatgttt atagaaaatc agccatgcca
     6001 tcgcccagat aaagctcaat gcaccggaga tgataaatgc catctgccag ctgtgcatta
     6061 cgattgccca taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa
     6121 agtaacctac cgcgatggaa cgctctttcg ccggaaacca ttcggagctg gctttcagac
     6181 ccgccgggat catcgctgct tccgcggcac cgactgcacc acgagcaatc gccaggccac
     6241 cccagctacc agccagcgcg gttgcaccac agaacacggc ccacagcaca gcaaacattg
     6301 cataaccgat tttcgtaccc agcacatcca gtacataacc tgctaccggt tgcatgaccg
     6361 tataagcagc agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact
     6421 cttccatcag agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg
     6481 tgccgagcgt caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa
     6541 acctcatcgt tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga
     6601 tgttagttac gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     6661 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     6721 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     6781 ctttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     6841 agcgaggcca gtactggcgc ggcctgcaga gagatttgcc actttgagag taattttttt
     6901 aactacgttt attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata
     6961 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     7021 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     7081 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     7141 gcggaagact atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa
     7201 tggcgcgcta tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcgtctgac
     7261 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     7321 cactggacgc acctcgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg
     7381 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     7441 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     7501 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     7561 ccgagctggc gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg
     7621 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     7681 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     7741 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     7801 ctggctggcg aacccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     7861 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     7921 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     7981 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     8041 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     8101 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     8161 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     8221 ctgctgagcc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     8281 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     8341 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     8401 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     8461 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     8521 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     8581 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     8641 tggcggatat tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg
     8701 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     8761 gtgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaat gagctgtgga
     8821 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     8881 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct
     8941 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     9001 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     9061 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     9121 agcaggatga tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc
     9181 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     9241 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     9301 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     9361 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     9421 acgacttcaa acagtacttt attgcccatg accagccgat ctatgaaaac ccatcgccgg
     9481 ggaacaaagc gggcggtatc accacgctgg aagataaatc ccttggctgt acccagaaag
     9541 cgggttccag cgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccggggc
     9601 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     9661 gctgccatat ggtgctgttc agtactggtc gtggcacgcc atacggcggc tttgtaccga
     9721 cggtgaaaat cgctaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     9781 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     9841 ccatcgttga gtttgccaac ggtaaacaaa cctgcaacga gcgtaacgac ttccgcgaac
     9901 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     9961 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc
    10021 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
    10081 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
    10141 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
    10201 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
    10261 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
    10321 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
    10381 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcaca
    10441 atgattgagg gtagttttct gcttatgaat ggcttgaata tcattcgttt ctggcggatg
    10501 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg
    10561 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
    10621 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
    10681 atacgccgat gataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
    10741 acatattaca ggccagcggg atcagcagca gagaaccccc cgccacgccg gatgcgccac
    10801 aggcacacag agaagccacc acgctcaaca gcagcgccgt gggcagatcg accgggatac
    10861 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
    10921 tgatggtggc acccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt
    10981 cacacagtgc catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca
    11041 cgccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
    11101 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
    11161 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
    11221 gaccaaaaat accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat
    11281 tcgacatatc gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga
    11341 ggccaattgc ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt
    11401 tggaaaccat gctcattacc agcccgcgca tcacttcgac aatgcctgac ggcggcgaaa
    11461 tatcacccgc gctactggat aaatgcaggg tagaagggaa ggcaaagctg aagactactg
    11521 cggccagagc agcagagaag gtacccagca gatagaggaa caaaataggg cggatattgg
    11581 ttttctgccc gtgctggtgg ttagcaatag atgccatcac cagcatcaac accaggatgg
    11641 gggcaacggc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
    11701 cgggttttga gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
    11761 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
    11821 gatcctttca ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
    11881 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
    11941 gctggatttt aaagagaggt catattttgc aacttaattg ttacatttgt ttgacataaa
    12001 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
    12061 tatgagaacg gttgcgctcc tttaatgtgt tatgccatat aaagtcactg gcgcagggag
    12121 cgcgcagggg gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat
    12181 cgccgctgcg cggttccctc agcttatccc ttgcggctac cgggtcgggc gcaaggtaac
    12241 gtccctgtaa gatgcgccct cgccgcgcat ccatgcgcgt cgccccggcc tccagggaac
    12301 gcttcggcga ttttgacgcc accaacaccc gtgcggtctt tttgtaaaga gctataagcc
    12361 gattacataa ttaatagctc acctttgtct ggaatgagtt atctctagta atggcttaat
    12421 atacagcgga tcattctccc cgctgcttat catgccgata attcacccag gcgttgataa
    12481 taaacgtcat caccagaatg ccaaacacca cgcccagcga gacggcgatt ggaatatggt
    12541 agaagtcgac aatcagcatc ttgataccga taaacaccag aatcaccgcc aggccatatt
    12601 tgagcatcga gaaacgctct gctacgcccg ccagcaggaa atacatcgca cgcaggccga
    12661 ggatcgcaaa caggtttgag gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag
    12721 ccggaatgct atccacggcg aaaatcacgt cgctcaattc caccagaatc agtaccagca
    12781 tcagcggtgt ggcgtacagc aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg
    12841 tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg
    12901 attcatcttc atgggcgagc gccatcttca cgccggtaaa cagcaggaag gcaccgaaga
    12961 tatacaggat ccagtcgaac tgtgaaatca gccagctgcc tgtgaagatc atgatggtac
    13021 gcagaacaat cgccccgagc acgccataca ccagcacgcg gcgttgtaat gccgccggaa
    13081 cagagaaata gctgaacaac atcagccaga caaagacgtt atcgaccgcc agcgatttct
    13141 caatcagata gcccgtgaga aaggccagtg cctgtggatc ggcaacggcg cgaccttcag
    13201 tttgcaccag ataccaccag aaagcggcgt taaacagtaa cgacagcgtc acccagacca
    13261 gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca
    13321 acaggtcgat agccagcata atggcgacaa caacagcgaa tccgccccat agcaacggcg
    13381 tgccgacagt attcatagaa gttccttaca cataaaaaaa cggccaacgt cggaagacgt
    13441 cagccgctgc tttttatgca tagacctcgc cttccggcaa ggtctcactt acaacaaaaa
    13501 aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt ttttcacaca
    13561 tcgtaatgac gatcgaccgg caatgaagtt actccccttt gcaggtaaca aagtatgaca
    13621 gaccattcag ccagtcaatg gtctgttaca taccttttac actgttttac tttggcaatt
    13681 atagttttac gctatctgcc ggaaaaatca ccccagtctg gcggcggatc tcggtcagca
    13741 gtttggcggt gatgcgactg accgccagcc ccggatgatc caccagatgc tcatccacca
    13801 gggtagcgaa cagctctgct tcgtagagca tggtattaat atgctgcggc tgggtgagat
    13861 cctgcatttg gctgccacgc ggcacgaagc acactttctg gcattcagac agtttttcaa
    13921 tcaccagcga tcctgcttcg ccctgaatct cgctcgccag gacagaatca ctgacttttg
    13981 agtgctgcaa ggtgacgctg aaatcaccgt aatccatcac caccacgccg tgggcatcta
    14041 caccgcttgc cagcaaactg gcggttgcct gcacgctttt cggctcacca aataacgcca
    14101 ccgccgacgc caggcagtaa aagccgatat ccataattga accgttagag aatgacggat
    14161 taaaggtgtt gggattttca ccgtccaggt aacgttgata gcgcgaggaa tactggcaat
    14221 agttgaaaaa gactttacgc aatttgccga ctttcggcag cgcctggcgc agcaaatgaa
    14281 agttcggcag gcaggcggtt ttaaatgcct caaacagcac cacctgattt tcccgcgcac
    14341 aggcaatggc ggcatccact tccgccagat tcgacgccag tggtttctcg caaatcacat
    14401 gaattttatg gctaaggaaa agttgtgtct gggaaaagtg cagggcattc gggctggcaa
    14461 tatacaccgc gtcaatggca tcgctttccg ccatcgcttc cagcgaggta aacagatgct
    14521 cgacagaaaa atcattggcg aagtgctgag cctgctcaag gctgcgggaa tatacggcgg
    14581 ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg
    14641 taccaatcac agcgaaacgt atcataacgc gtggatactc cataaaggga ttctgtcgtc
    14701 aatgtagcac gcctttttca tcttcgacgt gcgcagatac gcattattct tttgccggac
    14761 agccatgctc actaaacggc ccaatcatga ccgatggccc gagatatttc ggctggcgat
    14821 ggaggatttt ggcgtcaatc acggcgctga cacgagcgag tttctcccgc agttgagtat
    14881 aaaaaccgtg cttcatattg aggtcaggcg cgttaaaacc gatagcgtcg atgccgtact
    14941 gttttgccag ccagatggcg cgttgattat gaaattcttg cgagataatg gtgatatggt
    15001 tttcaccaaa aacctttttg gcacgcacta ccgaatccag cgttgagaat ccggcatagt
    15061 cgcagaagat cactttcgcc ggtacgcctt tagcgatcaa agcctgctgc atacctgagg
    15121 cttcatcgta atttttacgt ccgttatcgc cacttaccag tagccatttc acttttccgg
    15181 cgtggtacaa cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta cccggcctgg
    15241 cccccagcaa taagcccacg ttacgcgccg gaactgcgtt gacatcgctc caggtcagct
    15301 gcttgctggc gttgaccata acgcgatcgg caataaaaat ggtcgctcct gctataagca
    15361 acaacaaaca gccaatctta agagtccggc gagaaaaaca aatacggaga agaaggcggg
    15421 gctgcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga
    15481 aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc
    15541 cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca gcacaactgg
    15601 taaaacgctt tgttatgctc tttctccttc aggtgcgcca gttcgtgcac cacgatcata
    15661 cgtaaaaacg gttccggcgc gttgcgaaac acagtagcga cgcggatctc tgcttttgct
    15721 ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc gttttttagc
    15781 acatggatct tattgtcata catcacttta ttgatcggcg gggcattacg cagaaactga
    15841 tttttcagat cctgagtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc
    15901 ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg
    15961 gaaagtagct gctcgggata accctgaaga taagtaagat tgttcataac cgcccacgaa
    16021 aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt
    16081 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    16141 cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc
    16201 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    16261 cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg
    16321 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    16381 tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa
    16441 cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    16501 gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggag ctgttcgaga
    16561 aagtgctcgg cccgaccacc actacgctgg catggaaaaa agcgcgcctg attaattgca
    16621 ctttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    16681 ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctg gatattggcg
    16741 cacgcttctt tatgcaacat ctgccagaga atctcgaagg tgagattgtc gatctcggct
    16801 gtggtaacgg cgttattggc ctgacgctgc ttgataaaaa cccgcaggcg aaagtggtgt
    16861 ttgtcgatga atcgccgatg gcggtcgctt ccagccgaat gaatgttgaa accaacatgc
    16921 cagaggcgtt ggatcgctgc gagtttatga tcaacaacgc gctctccgga gtggagcctt
    16981 tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca gcaacatgcg ctgaccgata
    17041 acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac ggcgaactgt
    17101 atatcgttgc caaccgccat ctggattact tccataaact gaagaagatt ttcggcaact
    17161 gcaccactat tgcgacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc
    17221 gtcggtaaga ttggcagcaa cctgccggat gcggcgtaaa cgccttatcc agcctacgtt
    17281 cagcacgtta cccccaggcc ggataagacg cggcaagcgt cgcatccggc aatggctgct
    17341 catttctgga atcgcgtttc cataaaacaa aaaatccctg tcaccatcct ctacattctc
    17401 ttgtttagag tttttctacg tttatgctac atacgtgaag ttatgacgca caaagaatat
    17461 gatttcttta ccgccgttca tcgcactaag gggaaaaaat gattgcgctt tccaacatct
    17521 tgcaggcagg agaacagcta accgctgtgg caccttttct ggcgggtatt cagaacgagg
    17581 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    17641 ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga ggaatcagcg cctgaattcg
    17701 cagaatttaa tgcgatggtt gaagccatgc ctggcggtat agccgtgatt cgtaccctta
    17761 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    17821 cacgcgtttc gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    17881 gattcggcat ttctcccgcc ttgtttattg attaacgcga cgcgccggat gcgttgcatc
    17941 cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc
    18001 gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc
    18061 tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat
    18121 tgtccaccgc gaatacctgt gtttcgccgt tgatcaccac atgcagcccg tcatcgtcga
    18181 tcttctggta actgacgctt gggatcattt tcacaccgcg cgagagcagg gtagtgcgat
    18241 ggatccagcc ggtggttttg cctaacccct gtcctggttt gctggctttg cgctggagca
    18301 tcacaatctg ccgtgggcta cgagggatct gcattccctg cgggcttaag ccaccagcct
    18361 gttgcaggct actgtcgatc ccccattcat tacagaattc ggcgatgttc tggctggtgg
    18421 actcccctgg ctgactcaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga
    18481 tggccacctt tttacccacc ggcgctttgt cgcgcaatac atcgagataa ctcaatacct
    18541 tcggatgatc gatcccgtcg atgggcggaa tgcgcggcac gatcccgctg gcaaggatcg
    18601 tttcatcgaa cgactgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcaccc
    18661 ccgtcacttc gatcatccgg cggtaatagc gcagcgtttc gtaaaattcc tctttgccgg
    18721 ggatctgttt ggcgatatta aattgcccgc cgatctcact gtgggcatca aacaacgtta
    18781 cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt cccgcaccga
    18841 ccaccgccag attttttttc tgcacggcag gaaggattgg cattttggtt tcgtggcagg
    18901 cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct
    18961 gattgcagcc aatgcaggtg ttgatctcat cggctcgccc cgattgcgct tttgacagca
    19021 gttccgcatc agcaagaaac ggtcgtgcca tcgacaccat atcggcatcg ccgcgcgaga
    19081 gaatgtcgtc ggcaacctgc ggatcgttaa tccggttggt ggtcaccagc ggcagcgaaa
    19141 cgtggccttt cagtttgcgc gtgacccaag taaatgcgcc gcgcggcacg ggcgtggcaa
    19201 tggtcggaat acgtgcttca tgccagccaa tgccggtgtt gataatggtc gcgcccgccg
    19261 cttcaatggc ctgcgccagc tctaccgttt cggcaaaagt cccgccgtct tcgaccaggt
    19321 cgagcatcga cagtcggtag ataataatga agtcgttgcc gacgcgttcg cgcaccgcac
    19381 gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac
    19441 gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcggac cccattacct
    19501 ctacaccgtc gtatcctgcc tcccgtgcca gttgcgcgca gtgggcgaaa tcgtcgatca
    19561 gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca
    19621 atgcggaggg agcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca
    19681 gggctatttt gccgccttcc tgatgtaccg cttcggtaat ggtgcgatgg tgtgggatct
    19741 ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctgttaaa tctggtgcaa
    19801 tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgctgcca
    19861 gccgctcggc accgtccggg tattcctcca gcccggtgtg cattgagccc atcaacacgc
    19921 ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa
    19981 aattgtccag tatgtaaaat aattgttatg tggtcggatg agttctaatt tagccgtcgc
    20041 cggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag aatacgcccg
    20101 gtggtaacgc cggatgcgac gcttgccgcg ttttatccgg cctgggggat tgcacatgta
    20161 gggcggataa ggcgttcacg ccgcatccgc cagtggcacg gtgcagatgc cggatgcgac
    20221 gcttgtcgcg ttttatccgg ccaggaggat tgcacatgta gggcggataa ggcgtttacg
    20281 ccgcatccgc cagccgttgc aaattattgc ttacggaaaa aatcgttata caacatatac
    20341 aaatgcgcgg cactccagga gaaatttggt gcgccttgct gtgcgccagt cagcgggttg
    20401 taattctcct gaattgggcc atcggcggtt aaccctttgg cgtgccggaa gaacgtatcc
    20461 gccagtttca gggcatcatc gcgataaccg taacgctcca tccctttcag accaaaccag
    20521 aactgatcca cccatacgcg cccgcgccag tagatatcag cgccaaaggc cggattggtt
    20581 aacgccgccg ttcccagcgg aacaaaggta ttgaactctt taggatcgag catcaccttc
    20641 accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc
    20701 ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag cggtttatct
    20761 tcaatacgca cgtcatagaa gtactgcgta gccgggtcga acatacaggt gttgatgtag
    20821 tccgcgagct gctgtgccaa ctggcgatag cgcttagcct cttccggttt accgagaatc
    20881 gtcgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
    20941 tcctgcaata gcgagtaccc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
    21001 accgtccagt cgctacgttt gccgccgctt gcaacatatt tatccagctg ttctttgtcg
    21061 ataaacccaa agacggcggc atcatcacgc cccgattccc acgatgcagc aacctgtgcc
    21121 ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca
    21181 gactgcgtct cttctttgtc gcttttcttc accataaaca gcatctcgcc gctctcagtg
    21241 ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt gtgatcgcgg
    21301 ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc cacccaggtt
    21361 ttatcctggg tgacgttgta cacttccatc accgaccagg cggcaaggct gggtttggta
    21421 tttcgttcgt tccagttgcc accatcgcca ccacgctcgg ggctaagatt ccacgctatc
    21481 aggtcgggga caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag
    21541 gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc catcgcaaac
    21601 gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg cccggtcacc
    21661 gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agcgccagtt accgttgagc
    21721 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
    21781 cctttcttca ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc
    21841 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
    21901 gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta
    21961 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag
    22021 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
    22081 gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt
    22141 ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag atccagcggt
    22201 ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc
    22261 atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg aatactgtat
    22321 gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg gtcgaaattg
    22381 ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa gccgcccatg
    22441 gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
    22501 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc agtacggtta
    22561 atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat cagcagagcg
    22621 caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt attaccagta
    22681 gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt tcatgtagaa
    22741 gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga taccaaactg
    22801 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgcttggcgt aggtgtaagc
    22861 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccactttc
    22921 acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc gagcggcaaa
    22981 gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga tttccagacc
    23041 gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tcattgccgg
    23101 ataggtgtat ttgtcgtcat agcgatcgta cttgccaaaa tgtagccagg ctttgctttc
    23161 gtcatgatgt ccggcaggtg tggcggtgac gctataacga aagttgccgc ttaagttctg
    23221 caccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca gatcgggggt
    23281 gaagtcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac tgagcatgat
    23341 ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc cctcgaaacc
    23401 accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca cggtccagcc
    23461 ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac cttcaatata
    23521 ggtaccgtcg ctgtatttat gtttattctc accctctaaa tccatatatc caccaacccc
    23581 taattcgccg taaaaacgaa atgaaggaga agatgtcgag gtaattgcag gtgtttttgt
    23641 gtcgtcatgt tctgccggac gtgcttcttc tcctgccgca gaaaaggcgt agcaaaacgg
    23701 cagagcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa tccatgagct
    23761 aaagaaaaag tacagccttt tatcgttgta attataaaaa tataaaggca gaaactattt
    23821 tccctctcct catccaggaa gagggaaaat gcctgctcat tttttattat tctgcttaat
    23881 caacattgct ggctggcgtc gcttcaatat ggcgctcttc agccgttaat ccctttatat
    23941 atttactgta tttccaccac gcaaagccaa ggaagataac aataccgccg acgttataaa
    24001 aaatgatggt cagaatattc gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa
    24061 atatcgcaat cagcattgaa acaacgataa taccggtgcg gcgggagccc atacggaaat
    24121 cgcgtggcag gtgatctaat ttggcgcgta aattcaggta agccagcatg atgaataacg
    24181 gcggaagcat ggacgctgcg gcggtcatat taataatagt attcatcaga tcctgcacgg
    24241 tattggaacc gagcatcggg ataatcatca gcgggatgac gatcaggaac tggatccacg
    24301 ctgcgcgcgc cggaacgccg ttttcgttca gttcgacggt tttcttacca aagatgcctt
    24361 ccgggatttc ggagaagaaa attttcaccg gcgttgcggt ccacatcagc agggaaccga
    24421 acatcgcggt aaaggacacc agaccgacaa agcgattcat caacgcttcc ggtagaccaa
    24481 aatacgccgc catgccgtgg aatacctgca ccgatccgcc ggtaaatttc aactctttgc
    24541 tgctgacgaa gacgttaatc agcaccgagg agacggaata cagtacgccg ataaaaatcc
    24601 cggcgaggat gatcacttta acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca
    24661 cagcgacgga ctccgcgcca cctgcggcca taaagatcca ggtggtaacg ccgaggaacg
    24721 cccagttgaa gttcgggatc atcgcgtcaa cggtgatggg gtcagcaggc tgtacgccgc
    24781 caaccagcgc cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg
    24841 aagtgacgga ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga
    24901 aggcgaacag caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc
    24961 cgaggaaggc atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca
    25021 ggtttacgaa ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt
    25081 ttacccacgc gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga
    25141 tcagacagaa gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca
    25201 gtccaagctc aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca
    25261 gcgagagcaa gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc
    25321 gaaaatgtgt agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt
    25381 atggaaataa ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca
    25441 gataaaccga tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac
    25501 ggtttctccg cagcctttta aatattcacg gtcagtttca tcacgataat attccactac
    25561 ctgtaatgtc tctttcggcg catattcaat tttttgctgt ccttgcaggt agtaatgcac
    25621 ttcaaaatag cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg
    25681 gtaagtcaat gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc
    25741 aatcgcttca acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc
    25801 taagttatcg atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct
    25861 gcgccgaact catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc
    25921 aacgtaaagc cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg
    25981 ccccaggagt tggagccgag gccaagcagc tggtggtcga ggttcagggt gatgtcatca
    26041 ctgcgctgca gctcgttaca gtgctgggca gcgtggatgt tttcctgggt atagcgccag
    26101 gcgctgaagt taattgggcg ctgcggaacc accagcagac cgttgccgtg gcggttagtc
    26161 agtgccgtcc agcggacatg ctgacggttg ccgttgttct gcgggaaggg atagttctcg
    26221 aacatggcat cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg
    26281 tagttttcgc ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaatcccc
    26341 atggtgaaac cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc
    26401 gccacgttaa cctggccatc ggcagcgatg cgccagatgt aggtgcagcg catcccgaag
    26461 tcaaacaccg gcggggcaat aactgtgcgg ctgatgatca acacttcacc atcgctctgt
    26521 tctacggcaa agtcgcgcag atgttcctgc atgatctgca aatgattcgg ttgccacagc
    26581 ccttcgtact cctgcttgtg gttgtcgatc attggcttga agaagttgat ctttggctcg
    26641 cgggtcagca gcgactcgcc attcacctgc caggatgtcg gtttgccact catttttgag
    26701 aaggtgatcg cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc
    26761 agcggacgcg cattatttgg tgcgaaaggc actggttgcg cggtgttttc cttcagcggg
    26821 aactgataag tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc
    26881 accgtaatgt tgaggaacgc ttcgcgggcg tccagctgcg gcaacgtgat ctgcaagggg
    26941 gcttcgctgt tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct
    27001 tcgacgcgca cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt
    27061 tcgactttca gctcgccgtg agtcagatcc agcgcgtgga tttttaccgg cgcgataacc
    27121 tgtttgtact ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga
    27181 cagaagttat agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacgttg
    27241 ccgttgtcat cctgcgcctg gatcccgtgg tcgcaccatt cccagacata atgtccctga
    27301 atgcaatcgt gcttatagaa gacgttctgg tactccgtca gcccgcctgg tccgttcccc
    27361 atcgcatgag catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca
    27421 ttcatcagcg gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga
    27481 tcttcttcgt aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg
    27541 cggatgttac agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg
    27601 tttttctgcg cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga
    27661 tcgtcggtaa tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt
    27721 tccgccatca caaacaagcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg
    27781 tagtgagcgg tacgcacgga gttgatattg tgctgcttca tcaactggag atctttctcg
    27841 acgcgatcca ttccaacggc gcggcctttg cgatgatcgt tatcgtgacg gttgacgccg
    27901 tgcagcatca cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg
    27961 aagccaacgc gttgtggcac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg
    28021 accagatggt aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa
    28081 gcaaagctgg cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc
    28141 acgcgttcgc catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga
    28201 ttttccagca ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta
    28261 cgcacagtga aatcgttaat atgcgttagg tgttttccga ccagataaac atcgcggaag
    28321 atccccgctg accaccacat atcctggtct tccacgtagg tagagtccgc ccactgcatc
    28381 acgcgcacac acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg
    28441 gtcaggcgac tgcccttgct gaaacccaca tactgaccgt taacatagac ttcaaaatag
    28501 gtttcgacgc cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg
    28561 aaaatacgtt gataggcacc ggttgggtta tcgctgggga caaacggcac atcgatgggg
    28621 aacggaaaac cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg
    28681 gggacggtaa tatgccccca gtcagccatt aactcagagg tgaaggcttc tggtacttgc
    28741 agcggatggt caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg
    28801 gtttcgcggg caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgc
    28861 ggcgcaagtc ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc
    28921 ggtagagaga aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt
    28981 agtaaaattt ttactaaaaa gtagcaggta aatgcattta ctttaactgg atcacgaaaa
    29041 taaacggcag cactgtccgg caaaaacgat tgccggacag tgtgtaaggg gatttaacgg
    29101 gtcgtgccgc gcagttttaa tttgctggga acgaagacta acagcggcag cgcgcgacca
    29161 tcgcgggctt tttcatacac caggttaacg ccctgacttc ccatcatctc ggaatggatg
    29221 cgcacggtgg agagcggcgg aaaggtaaat cgcgcggtgg ggatatcgtt aacgctgata
    29281 agcgaaatat cctgtgggat gttcaggcct cgttcatgaa ttgcccgcag tacgccgata
    29341 gcaatggaat cggaagcaac aaacagtgcc ttcggatagt cttcccgcgc cagcatttgt
    29401 tttgccagtt cataacccga cgaactggaa aaaccgccgc gccagatatc ctcttcgcgt
    29461 accacttgtt tcagtcggcc atattccgca aaggcgacct cacgaatatc cgccttgcca
    29521 ggctcatctt caccgccaat aaaaccaata cgattaacgc cctggttgat atagaagtcg
    29581 atgatttctt tactgatgcg tgccagatcg atatccaccg catcgtaacc gctgccgggt
    29641 tcgtgaaagt cgataaaaca gatattgtcg gtcaacgcac aggcggcggc gcgcagggcg
    29701 ggcgtgggtt tgccgacaat taaaataccg gtgacgtttt taatgtctgg taatccgctg
    29761 tgttcataac agttggtgag ctcaatgccc agcttttcgc actgagtttc aatgccgtgg
    29821 cggatcgcca gatagtaagg atcgttgatc tccagctcct gttggtagct gtagatagcc
    29881 agaatatggt gttggttgac tgcacctgtc tggagtttac gggcactact ggtcttgtac
    29941 tccagctttt cggcgatctc gagaatgcga tgtttcgtct cttctttcac attcaatgtc
    30001 ggatcgtcat ttaagaccct ggatactgtc gccagggata cgccagcttc gattgcgatg
    30061 tcttttagtg ttgccatgtt tacctttttt actttccctg atgtgcgccc ccattgtaga
    30121 ggaagtgctt atcgggtcac ctgttttcag taaataaagc gcgtttcttt gacgctgtaa
    30181 cggtaacaca gcggctcacg aatgtgatca cgcctgcatt ctccattttt agcctacagg
    30241 attgagaaaa acttttgcta tagtttagta aaattttacc cgaagggagg aaacaatgga
    30301 aaccgtggct tacgctgatt ttgcacgtct ggaaatgcgc gtcggaaaga ttgtggaagt
    30361 gaaacgccat gaaaacgccg acaagctgta catcgtacag gttgatgtgg gggaaaaaac
    30421 actgcaaacc gtgaccagcc tggtgccgta ctacagcgaa gaagagctga tggggaaaac
    30481 ggtggtggtc ttatgcaatc tgcaaaaagc gaaaatgcgt ggtgaaacgt cggaatgcat
    30541 gctgttgtgt gcggaaaccg atgacggcag tgaaagcgtg ttgttaacgc cggagcggat
    30601 gatgcctgcg ggcgtacgcg tcgtataaaa agatcggatg gcgacgtcgt atcgccatcc
    30661 gatttgatat tacgcttctt cgacacttac tcgcatggcc gccagcgcct tacgcgccgc
    30721 tttgatcacc agttcacact gttcaatggt cagtgtcagt ggcggttcaa tgcggatcgt
    30781 tttggcgtta ttgagcgttc cggccaccag tacgcgctgg cggaacatct cgctggcaaa
    30841 gttatagccg atttcgttat caacaaactc aattgccatc aacatccctt taccacgcgc
    30901 ttcctgtacc agatcgggat attcccgcgc cagttgacgg aaaccgtcca gcaacatatc
    30961 gcctttttgc tcagcctgag ccggtaagtt ctgctccagc aacacattga tggtcgccag
    31021 cgccgccgca caggccagcg ggttgccgcc aaaggtggtg gtatgcagga atgggttgtc
    31081 gaacagaact gaaaacacct cttcagtggc gatggtcgcg ccaatcggca tcacgccgcc
    31141 gccgagcgct ttggcaaggc agaggatatc cggctgtacg ttctcatgct cgcaggcgaa
    31201 catcttgccc gtgcgcccca tgcccgtttg tacttcatcg aggatcatca gtgcgccgaa
    31261 ctcatcgcat agcttacgta cggcggtgag atagcccggc ggcggcagaa ttacgccacc
    31321 ttcaccctga atcggttcga ggatcaccgc agccacatca tcaccggttt ttttgcactc
    31381 gttaagagcc gtgcgcatgg cttcgatatt gccaaacggc acatgacgga agcccggcag
    31441 cagtggcata aacggtttgc ggaaggtcga tttcgccgtg gccgacagcg cgccaagtga
    31501 tttaccgtgg aacgcgccgc tggtggcaat aaaagtaaac ttgccgcgcg gtgactggta
    31561 agctttcgcc agcttcagcg ctgcttcaac ggactcggtg ccgctattac agaagaagct
    31621 gtatttcagt ttaccgggcg ttagcgcagc aagggttttc gccaacatcg cccgtaacgg
    31681 atcgagcagc tcctggctgt gcagcggttg tttcgcaagt tgattctgta cggcggaaac
    31741 cacaactgga ttacggtgcc ccacgttgaa aattccaaaa cctcccaggc agtcgataaa
    31801 ctcctgtccc tgggtgtcga caagcgtatt taaacctccc gcttgccact ctacggctcc
    31861 gtaatccccg ccggcggtaa cagatttgcg atactctaaa aaccccggat tgacatgctc
    31921 tttgaagtat tcaatcacct ctcggttaag tgctttcatc tcctcatgat ccagcgttcg
    31981 cttctcaatg agattcaggg cgtgggcgct gcacgctaaa gccgatgcgc tcgaaggtaa
    32041 cctgttcaaa atatgctccg gaggctcgcg tatcacatga taccgattta agtattgcag
    32101 ggattgcgcc actccggctg cgatcgcgaa aatcgggata aacacaaatt cggttatcat
    32161 ttgtgcaata tttaatgcta tctgttaaca tttgttgtta ttttgcacgg tttttgtcgg
    32221 gtatggattt gatgattgct gcgcaaatgc acgttttcag ggcaataatt gcctgatttt
    32281 tgggcaagat aattaacgtt ttcgtatagt aacagagtga aagtatttta aatcaaagaa
    32341 ttagatttgt gctttaatcg caaattgcga tctaaatcaa attaatcggt taaagataac
    32401 cgcagcgggg ccgacataaa ctctgacaag aagttaacaa ccatataacc tgcacaggac
    32461 gcgaacatgt cttctcatcc gtatgtcacc cagcaaaata ccccgctggc ggacgatacc
    32521 actctgatgt ccactaccga tctgcaaagc tatatcactc atgctaatga cacttttgtg
    32581 caggtgagcg gctttacctt gcaagagtta caagggcagc cgcacaacat ggtgcgtcac
    32641 ccggatatgc caaaagcggc gtttgcggat atgtggttca ccctgaaaaa aggggagccc
    32701 tggagcggca tcgtgaaaaa tcgccgcaaa aatggtgacc attattgggt gcgggccaat
    32761 gcggtaccga tggtgcgcga gggaaaaatc agtggctata tgtcgattcg tacccgggcg
    32821 acggatgaag agattgcggc ggtggagccg ctgtacaaag cgctgaacgc cggacgtacc
    32881 agtaagcgta ttcataaagg cctggtggtg cgtaaaggct ggctgggtaa actgccttca
    32941 ttaccgcttc gctggcgggc gcgtggagtg atgaccctga tgtttatctt gctggcggcc
    33001 atgctttggt ttgttgctgc cccggtggtg acgtatttcc tctgtgtgtt agtggtattg
    33061 ttggcaagcg cttgttttga atggcagatt gtgcgcccga tagaaaatgt cgcccgtcag
    33121 gcactgaagg tggcgaccgg agagcgtaat agtgttgagc acctgaatcg cagcgatgag
    33181 ctggggctga cattacgcgc ggtagggcag cttggcctga tgtgccgttg gctaattaac
    33241 gatgcctcaa gccaggtgtc cagtgtcaga aatggcagtg agacgctggc gaaaggcacc
    33301 gatgaactga acgaacatac ccagcagaca gttgataacg ttcagcaaac ggtggcgacc
    33361 atgaaccaaa tggcggcgtc ggtgaaacag aactctgcca cggcgtcggc tgccgataaa
    33421 ctttctatca ccgccagtaa tgcggcagtg cagggtgggg aggcgatgac cacggtgatc
    33481 aagacaatgg acgatatcgc cgacagtacc cagcgcattg gcaccattac ttcgctgatt
    33541 aacgatattg catttcagac caatattctg gccctgaatg cggcggtgga agcggcgcgt
    33601 gccggcgaac agggcaaagg ttttgcagtg gtggcagggg aagtgcgtca tttagccagc
    33661 cgcagcgcta atgctgccaa cgatattcgc aagctgattg atgccagtgc tgataaggtg
    33721 caatccggtt cgcagcaggt acacgccgcc ggacggacga tggaagatat tgtggcacag
    33781 gtgaaaaacg tcacccagtt gatcgcccag attagccatt caacgctgga acaggccgat
    33841 gggctttcca gcctgacccg tgcagtggat gagcttaacc tgatcaccca gaaaaatgcc
    33901 gagctggtgg aagagagtgc gcaggtgtcg gcgatggtga aacaccgcgc cagccgactg
    33961 gaagacgcgg tgacggtgct gcattaatcg gtatgccgga tccggcggtt ggagcacaat
    34021 gcctgatgcg atgctggcgc atcttatcag gcctgcgggg tgtgtagcgg gtgtaggccg
    34081 gataaggcgc gtcaagcgtc gcatccggca attgcaccgc gccactggcg gatgcggcga
    34141 ttaatccagt tgcgtaatat caaaagcggc gcggtcgatc accgcaatga tcttttttat
    34201 ttgtgcatca ctgatatcgc tctggttgac gcgtaaatcc agcaccgctt taaaattatc
    34261 cagcgcccgc ttcatttgcg ggttctggcg cagcgccgcg ccaacgcaac gcgctttgat
    34321 gcgttcttca atcatctcca cctgttcgcg gttttcttcc agccactgcg cgccttgttc
    34381 ggtcagcgca atctgcttct taccaccttc ctcttcgcgg atggtaatca gcgactgctc
    34441 ctgcagaaaa tccagcgttg ggtagatgac gcccgggctt ggggtgtaat tcccctgggt
    34501 taggttctca atcgctttaa tcaattcgta accgtggctg tcatcgcgcg agagaatatc
    34561 cagaatcacc agacgcaatt caccgtgacc aaagaaacgt tgccgacgac cgccgccgcg
    34621 accgtggcga ccaccgcagc attgaccgtg ttcatgctgg tgaccgtgtc cgcagtgctc
    34681 gtgttctgac ttctcacctt tgcagcagcc ctcatggcgt ggctggcctt catgtttaca
    34741 acacccttcg tgatgatggc tcatatcagc ctccttttgc ttgattagat atatctaatt
    34801 tatatctgat gtgagcatta ctgcaagtac aaaaatgatt tttaaacgtg gaagcacctc
    34861 acatccaggc cggataagac gttcacgccg catccggcag cattgctatg gcactgaatg
    34921 ccttcacata cttcagatat atcagttcta caaatcgctt gcatttatca tgattaacaa
    34981 tcattatcat ttacgaaaat tatttagata tatctgatta ctatcacgaa ggcgataaca
    35041 atgaataact ccccccgcta cccgcagcgc gttcgcaatg atctgcgctt ccgtgaactg
    35101 actgtgttac gcgttgagcg catcagcgcc ggttttcagc gcattgtcct cggcggcgag
    35161 gcgctggacg gttttacatc gcgcggcttt gacgatcaca gcaaactctt ctttcctcaa
    35221 cctgacgctc actttgtgcc gccaacggta acggaagagg gcatcgtctg gccggaagga
    35281 ccacgcccac cgtcgcgtga ctatacgccg ctgtatgacg aactacgcca tgaactggcg
    35341 atcgatttct ttattcacga cggtggggtc gccagcggct gggcgatgca ggcgcaaccg
    35401 ggcgataaac ttacggtggc aggtccgcgc ggttcgctgg tggtgccgga agattacgcg
    35461 tatcagctgt atgtctgcga tgaatccgga atgcccgcac tgcgccgccg cctggaaacg
    35521 ttgagcaaac ttgccgttaa accgcaagtt agtgcgctgg ttagcgtgcg ggataacgcc
    35581 tgtcaggatt atctcgcgca ccttgatggt tttaatatcg aatggctggc acatgatgag
    35641 caggcggtag atgcgcgtct ggcgcagatg caaatccctg ccgatgatta cttcatctgg
    35701 ataaccggcg aaggtaaagt cgttaagaat ttaagccgcc gctttgaagc ggaacagtat
    35761 gacccacagc gggtacgtgc agcggcttac tggcacgcaa agtaactgat atataaaaag
    35821 aaaaaggctg acgatttctc gtcagccttt gctatatctg gtggcccctg ctggacttga
    35881 accagcgacc aagcgattat gagtcgcctg ctctaaccac tgagctaagg ggccatggta
    35941 gcggattata aagtaactcc gtgtcgcaat ccagccatta ccgtgcgcct gctgttttta
    36001 taaacaatgt attttcaatc cgttatactt ttcctggtga tgttagaaag gagtaaatat
    36061 ggttgaggat attttggctc cagggttacg ggtcgtgttt tgcggtatca actctgggct
    36121 ttcatccgcc gggactggtt ttccctttgc tcatccagca aatcgcttct ggaaggtgat
    36181 atatcaggcc gggtttaccg accgtcagtt gaagccgcag gaggcacagc atctgctgga
    36241 ttatcgttgt ggcgtcacca aactggtaga ccgtccaacg gtgcaagcca atgaagtttc
    36301 aaagcaggag ctgcacgcag gtgggcgtaa gctgattgaa aaaattgaag attatcagcc
    36361 gcaggcgttg gcgattctgg gcaaacaagc atatgaacag ggattcagcc agcgcggtgc
    36421 acagtggggg aaacaaacgc tcaccattgg ttcgacgcag atttgggtgc tgccaaatcc
    36481 cagcggttta agtcgcgttt cactagagaa actggttgaa gcgtatcgcg agctggacca
    36541 ggcgctggta gtgcgtgggc gataaaaaac gccaccgaaa aggtggcgtt tgtgcaaagg
    36601 gactagtgcc gggtgcggcg taacgcctga tccggcctac cgattaatcg tccaggaagc
    36661 tacgcaacac ttcagaacgg ctcgggtgac gcagtttgcg cagcgccttc gcttcgatct
    36721 gacggatacg ttcgcgggta acgtcgaact gtttacccac ttcttccagc gtgtggtcgg
    36781 tgttcatatc gataccgaaa cgcatacgca gaactttcgc ttcacgcgcg gtcaggccag
    36841 ccagcacgtc gtgcgttgcc gcacgcaggc tttcggtggt cgcagaatcc agcggcagct
    36901 cgagggtggt atcctcgatg aaatccccca gatgcgaatc ttcatcatca ccgatcggcg
    36961 tttccatgga gattggctct ttggcgatct tcagcacttt gcggatcttg tcttccggca
    37021 tcagcatacg ttcagccagt tcttccggcg tcggttcacg gcccatctct tgcagcatct
    37081 ggcgagaaat acggttgagt ttgttgatag tctcaatcat atgcaccgga atacggatgg
    37141 tgcgcgcctg atccgcgata gaacgggtga tcgcctgacg gatccaccag gttgcgtagg
    37201 tggagaactt gtaaccacgg cggtattcga atttatcaac cgctttcatc agaccgatgt
    37261 tgccttcctg aatcaggtca aggaactgca agccacggtt ggtgtatttc ttggcgatag
    37321 aaataaccag acgtaagttc gcttcaacca tctctttctt cgcacggcgg gctttcgctt
    37381 caccgatgga catacgacgg ttgatatctt taacctgctc gatggtcagg ccggtttctt
    37441 cttcaatctg ctgcagtttc tgcagggcgc gatgcacttc ttcagagaca tcgtgcagtt
    37501 tttccgacca cggcttgttc atcgcaattg ccgcgttgaa ccaggtatcg ctggtttcgt
    37561 tgccggtaaa cagggtaatg aagtttttct tcggcatttt gcactgctca acgcagagct
    37621 tcatgatcag acgttcttgc gtacgaacgc ggtccatcat gacgcgcatg ctgttgacca
    37681 ggtagtcaaa ctgcttcggc accaggcgga actgtttgaa tacttcagac agtttcagga
    37741 tctcttcctg agcggcagcg tgactgcgac ctttcgcttt gatggtgtca cgcgttacaa
    37801 cgtactgagc gcgtagttcc gcaaattttt cgcgagccag ttccgggtcg atgctgttgt
    37861 catcatcggc gctgtcgtca tcgccatctt cttcgtcttc atcttcgtca tcgtccagat
    37921 cttcctggga aagctcagaa ccgacgtgag tggcggtagg tgccagatct tcttctgcgt
    37981 tcgggtcaac aaagccggtg atcagatcgg acagacgcgc ttcttctgct tcaacacgat
    38041 cgtactgttc cagcagatag gtgatcgctt ccggatattc agcaacggag cattgaacct
    38101 ggttgatccc gtcttcaata cgcttagcga tgtcaatttc gccttcgcgg gtcaacagtt
    38161 caacggtgcc catttcacgc atgtacatgc gtaccgggtc agtcgtgcgc ccgatttcag
    38221 attccacgct ggaaagcacc tgcgcggcgg cttcggcagc atcttcgtcc gcggtgtttt
    38281 cagccagcat cagatcatcg gcatccggtg cttcttccat cacctgaatg cccatgtcgt
    38341 tgatcatttg gatgatgtct tcgatctgat ctgaatcgac gatatcttcc ggcagatggt
    38401 cattgacctc ggcataggtc agatagcctt gctccttacc acgggtgaca agaagtttca
    38461 gctgtgactg cgggttttgc tccataagac ggtatccaca cttatttcat gaggttggtg
    38521 ttgggcgatt gacccgatgt ccttcagccg ttaacgctga aatccaacgg ttgtttaccg
    38581 ccaacgataa ttacgagggc gtacttatat atttgccgct gcctctcagt gcggctgtcg
    38641 ggggcttccc gatcgctctt cggcacttaa gccgttaaat cactttttcg ccagctcctg
    38701 gtttaatgtc cagagctcca ggcgttcttc gttgcttaaa ccatgcgtgc gctcacgagc
    38761 gattaactct tcctggcgca gttcaagcag cgaatcaaac atatggttga gtgagtcggt
    38821 gaaggtttgc tcagcaatat tcttatctgc tatatcgtcc cacatcgaca gtttttcaag
    38881 ggtggcagca ttatttgtac cacgatagtg ctctaaaagt tgcccggtgg tcagacctgg
    38941 ctgggagaga caagtgttga ccagttctct gaataagcca agtccaggga gcttattttc
    39001 atccagattc tcaagcggcg ggaccaacgt cgctaattct ggattttgca ccagcaaccc
    39061 tataagtata cgcatggtcg tgcgttttag ctgcggaaca gggcgagaaa cgccgctctc
    39121 tgccgctttt ggcattaatc gttcaagctg gctgtcatca agtatgccta atttgttgcc
    39181 taattcctga cgaagatata ttcgcagcgt ttcgcccggc acttgcgata tcaatggcag
    39241 tgccagcgta ctcaaacgtg cgcgcccgtc aggggtactc agatcaactt gcggcatcag
    39301 gctgttaaac agaaatgcgg aaagcggcat cgcctgctcc atccgcgctt caaacgcttc
    39361 tttaccttct tttcgtacca gcgtgtcagg gtcttcgcca tccggcaaaa acataaagcg
    39421 tagctgacgg ccgtctgtca tgtaaggcag cgccgtttcc agcgctcgcc atgcggcatc
    39481 gcggcctgca cggtcgccgt cataacagca aatgacattg ttggtcgcgc ggaacaacag
    39541 ttgtatgtga tcggcggtgg ttgacgtacc taacgacgca acggcgtaat taatgccgta
    39601 ttgcgccagc gccaccacgt ccatatagcc ttcgaccaca agcagacgat tgggttcagc
    39661 gttatcctgc tgcgcttcat aaagaccgta aagctggcgg cctttatgga aaatgtctgt
    39721 ttccggcgag ttcaggtatt tgggggtatc gttgcccagc acgcgcccgc caaaaccaat
    39781 cacccgaccg cgtttatcgc gaatggggaa catcacccgc tcgcggaaac gatcgtaact
    39841 gcgtccctga tcgttagtga ccaacatccc cgcatcaatc aatgactggc gattttctgg
    39901 attgccgcca aaccgcttca ggacgttgtc ccagccgggg ggcgcaaaac caatcgcaaa
    39961 gcgagcgata acctcgtggc ttaatccgcg tttttccaga tactggcgcg cagacgtggc
    40021 aacaggttgt tgtaaagatt gttggtaaaa cgtattcaga ccgtccatca actgataaag
    40081 cgtttgcctc tgatggcgct cgatctggct ggggccgctg cctgcttcaa atggcacttc
    40141 aagattgtgc attgctgcca gctcttcgac cgtttcaacg aactcgagct tgtcgtagtt
    40201 catcaggaag tcgatcgcgt tgccgtgcgc gccacatcca aagcagtggt aaaactgttt
    40261 ctcaccgtta acggtgaatg acggggtttt ctcgttgtgg aatggacaac acgcgtggaa
    40321 attcttgccc tgctttttca gcttcacacg ggcatcgatc agatcgacga tgtcagtgcg
    40381 tgccagcaga tcattaatga atacgcgtgg gattcgtcca gccataggcc ccgattttta
    40441 gcaattcata aacgaaaata agccgcgcat tcctttcgga agcacggcct tacaactaca
    40501 actcggtctg atcggagagc aacgctctcg gggaattagt acagacgagt gcggcgtgcg
    40561 ttttcgcgag ccagtttctt cgcgtgacgt ttcactgcag aagctttagc gcgcttacgt
    40621 tcggtagtcg gtttttcata gaactcacga cgacgaactt ccgccagaac acctgctttt
    40681 tcgcaggaac gcttgaagcg acgcagagct acgtcgaacg gctcgttttc acgtacttta
    40741 attaccggca tgtgcctctc acctttgatt aattcggttt gccgctggca tcaacgccag
    40801 cttatttcaa aatggtgcgg aattttactg caattgctgc tgctttgtaa agcaccgcgg
    40861 ccttttttga aagggacttt tataagggtg aggagtatac acgaggcttt ctccaggggc
    40921 gaacaaagtt ttacatcaac ccgcattggt cctacactgc gcggtaataa agcgaggtaa
    40981 aacaagtcat gcgtgtactg ggtattgaaa cttcctgcga tgaaaccggc atcgccattt
    41041 acgacgatga aaaaggtttg ttagccaacc aattgtatag tcaggtgaaa ttgcacgctg
    41101 actacggcgg cgtcgtgcct gaactggcct cccgcgatca cgtgcgcaaa accgtaccgt
    41161 tgatccaggc ggcgctaaag gagtctggtt taacggcaaa agacattgat gctgtggcct
    41221 ataccgcagg ccctggatta gtcggcgcac tgctggttgg cgcaaccgtg gggcgttctc
    41281 tggcgtttgc ctggaacgtt ccggcaatcc cggtacacca tatggaaggg catctgttag
    41341 cgccgatgct ggaagataac ccgccggaat ttccgtttgt cgcgctgctg gtgtccggcg
    41401 gtcatacgca gttaatcagc gtgactggca ttggtcagta cgagctgctc ggcgagtcta
    41461 tcgatgatgc cgccggtgaa gcgtttgata aaaccgcgaa gctgctgggg ctggattatc
    41521 ctggcggacc gttactgtcg aaaatggcgg ctcagggtac tgccgggcgc tttgttttcc
    41581 cgcgtccgat gaccgaccgt ccggggctgg atttcagctt ttctggtctg aaaacctttg
    41641 cggcgaacac gattcgtgac aacggcaccg acgaccagac gcgtgctgat atcgcccgcg
    41701 cctttgaaga tgcggtggtc gatacgttga tgattaagtg taagcgagcg ttggatcaga
    41761 cgggctttaa gcgactggtc atggcgggcg gcgtgagtgc taaccgcacg ttacgggcga
    41821 agctggcgga aatgatgaaa aaacgccgcg gcgaagtgtt ctacgcgcgt ccggaatttt
    41881 gtactgataa cggcgcgatg atcgcctatg ccggaatggt gcggtttaaa gcaggcgcga
    41941 cggcggatct cggcgttagc gtgcgtccgc gctggccact ggcagagtta ccggctgcgt
    42001 aaaactatcc ggtgccggag agcaatttcc ggcaccgtcc tcacttaaag caacaccacg
    42061 ggcatccaca gcaagccggt aatcaccagc aacacgagga atatcagtcc aaaaatcgcc
    42121 cccagtcgcc agtaatccac cgttggcaga taaccactac cgtagtaaat cggactgggt
    42181 ccggtggcgt atggtgtaag aatgctcccc agaccaattg ccgcgccaac catcaggcag
    42241 aacaccggca gcgggatttc cggcatcgcc agcgcagcgg caatcatcat cggtgcgagg
    42301 gcggaggtat acgccgtggc gctggcgaaa aagtagcgca gcagataaaa caccacaatc
    42361 aacgccacca tcaccatcgt tggcgaataa ccgcttaagc tgcctgctaa cagtttgcca
    42421 aaccagctaa taaaaccagt gttgttgagt ccggtagcga gggtgataag cgaggccagc
    42481 cagaagaaaa cgttccacgc cgctttatta ctgacaatgt cgtcccaact gataatgcgc
    42541 agaagcagca tcagtgccac cacgctgtaa ccgaccatcg cggcatcgat ataatcaccg
    42601 ccgaaaatcc acagcaccag cgcgcctacc atcagcccca gcatccgttt ttcacgcgaa
    42661 cagagcgggc ccattgcctg cagttccgtc tctgcccagc gcggcacctg atcgccagac
    42721 ttcagtaccg gcgggtacag cacataagcc agccagggaa ccagcagcac cagcaaaata
    42781 ctgagcggca acatcccgag gaaccagtcg ccccaactca gcgtggcgtg agatgcgctt
    42841 ttcatcagtc caattaacag caagtttggt gccatcgccg tcaagaaaat ggcgctggtc
    42901 acgcagtcgg caacaatccc catccacatg atgtacgagc caatgctgcg cgaactgctg
    42961 tcgtttggtt gtgattgata gagcggtggc aggttacgga tgatgggata gataatcccc
    43021 gcaccacgcg ccgagttgga cggtgttaca ggtgcgagga tcagctcgga gaacatcacc
    43081 gcatagccga gaaacagcgt gcgatgtccc atctttttca ccagaatcag cgcaatgcgg
    43141 cgtcccagcc cggttttttc atagcctgtg ccaaacataa aggcggcgaa aatcagccag
    43201 ataaccgaat tagaaaaacc ggaaaccgcc cacgagaggg attttgcagt gaatttaaag
    43261 cctggctggg cgagctgttc ggggctgaac agcagccagg gagagagtat ggcgatgatg
    43321 gagataccca ccatcgccac cacggcaccc ggcacgggtt cgaggatcag tccaacgatc
    43381 acgccagtaa aaacggcaaa gtagagccag gtatgactct ccagccctgc gggaaccgga
    43441 attagagcaa taatggcgat gaccgccagc ggcgcaaggt atcgccacca ttcagtggaa
    43501 ggtttcatcg tgttatgcct gcgaaaaagg agagggggcg agcctctccg ggagggttat
    43561 ttgatgtaat ggacgtgctc gcagatctct tccacgatgg gatcgcggcg ttcggcgaac
    43621 agctttttgt tttcggctat caggttgttg ccgtgggtat caatagagac aatcagcggg
    43681 ccgaactctt tgacccggca gacccacagt gactccggca tcccgagttc tgtccagtgc
    43741 acttcttcaa tctcttccac ctgggttgcc gccagcaccg cgcagcctgc cgggaaaatc
    43801 acatgtagcg ccttgaattt ctggcagcct tcttcggtca gcggccccat tccgcctttg
    43861 ccaaccacca gtttcacgcc ggtctgctcg ataaattcac gttcaaaact ttccatacgc
    43921 atactggttg tcgggccaac ggaaaccatc tcccatttat cgccgttttt gcgcacgatg
    43981 gggccagcgt ggaaaatcgc tttgccgttg agatcgtaag ggatcggacg tttcagttcg
    44041 atcaagcggc ggtgacaaac gtcacggcag gtcaccagcg taccggtcag gtagatcaca
    44101 tcgccgacgc gaatatcttg cagatcttca gctttgatcg gggttgtcag gatctttttc
    44161 ataacgcgct ccgggtgtga gacagatttt caaaggtgag atcggcatga accagcagcg
    44221 tgccgcgacg atgcgcccag cagccggtag agacagcaac gccgatggtt gacggatggc
    44281 gggcggcaga ttcgatatgc acgcccatca ctgaactgtt gccggtcagc ccttgtggac
    44341 caatccccag acggttgagt ccttcttcca ggcgtagctc cagctctgcc gcttttggat
    44401 tgggatggcg gctgccaatc ggacgcaaaa tggctttacg cgagagtacg gcggcggttt
    44461 ccaccgaggt ggcgatgccc acgcccacca gtaccggtgg acaggcgttt acggcgaggg
    44521 tggagatatt ttcgaagacg aatttcacca cgccttcgta gccttctgac ggcattaaca
    44581 ctttcgagcg gccaggtagc gtgcagccgc cgcctgccat gtaaacttcg atttctgcat
    44641 cgtcattgtc ggggatgatg tcccaggtga cccacggtac gccgctaccg gtatttttgc
    44701 cggtgtttac ttcgtcaaaa atttctaccg cattgtgacg tagtggcgct ttgacggttg
    44761 cctcttccac ggcttgtttg agtatgcttt gcagctcgcc aagcagtggg aagcgggaac
    44821 cgactttaac gaagaacata atctccccgg tgtcctgaca ggcaggacga ttcaggtcaa
    44881 tcgctttttg catgttgtcg aacatcgtat ggtagataat tttccccatc gacgacgttt
    44941 cggcatcctt tagctgtttt agtttatcca ccacgtcatc aggcattcgg gtagaaatca
    45001 tggcggtaaa gttagcgaca atctctgtca acttattcac tgcctgttgc ttattacttt
    45061 cgctcatcat ggcaactcca gtttttgtat ttgtctggaa aaattcaccg ccacaaacta
    45121 ccactaaaaa tgtggtttta tattttttgc caccgtgaat atgtaattaa cagttcgtga
    45181 atcaacgcga aaataagaat gaatattgcg ccagagcagg gaatggcaat gttttgcgaa
    45241 atacactgcg cttatcacaa ctgcggataa ttaacgatgc tgaatagctg gcctttagcc
    45301 aaagacttgc aggtactggt agaaatcgtc cacagcggga gttttagtgc tgcagcggcg
    45361 acgcttgggc aaacgcctgc atttgtcacc aagcggatcc aaatccttga aaatacgctg
    45421 gcaacaacgc tgctgaaccg ctccgctcgc ggcgtggcgc tgactgaaag tggccaacgt
    45481 tgttacgaac atgcgctgga aatcctcacc caatatcagc gactggtcga tgacgtcacg
    45541 caaatcaaaa cgcgcccgga ggggatgatt cgtattggtt gcagttttgg ttttgggcgc
    45601 agccatattg cgccagccat taccgaactt atgcgcaatt atcctgagtt acaggtgcat
    45661 tttgaattgt tcgatcggca aattgattta gtgcaggata atattgatct ggatattcgt
    45721 attaatgacg aaattcctga ttattatatt gcgcatctgt taacgaaaaa taaaagaata
    45781 ttatgcgcag cacctgagta tctgcaaaaa tatccgcagc cacaatcatt acaggaatta
    45841 agtcgtcatg actgtctggt gaccaaagag cgcgatatga cccacggaat atgggagttg
    45901 gggaacggtc aggagaaaaa atcggtgaag gttagcgggc atctttcctc caatagcggc
    45961 gagatagtgt tgcaatgggc gctggagggc aaagggatta tgctgcgttc cgagtgggat
    46021 gtgttgccgt ttctggagag tggcaaactg gtgcaagtat tgccggagta tgcacaaagc
    46081 gccaatatct gggctgttta ccgggagccg ctctatcgca gcatgaaatt acgtgtctgc
    46141 gtggaatttc tggcggcatg gtgccagcaa cggctgggca agcccgatga aggctatcag
    46201 gtcatgtaga tccaccagaa atcactcggg atccttttcg cgctttcttt tgaatttcgt
    46261 ccagattttt gtctcctgac gacgccacag acgctggatg ttgtcatgat gacgcagcag
    46321 gatcaggcaa gagagcatcg aaaccgggaa ggtgaattgt ggcttaaacc accatacata
    46381 aaacggagca atcagtgcac tgacaatcgc tcccagcgac gagtatccgc tcaatagcac
    46441 ggtcagtaac caggttcccg ccattactcc ggtgagatcc cagccaatgg gtgcgatggc
    46501 accaaaagcg gtagcaacgc cttttcctcc tttaaatccg aagaaaacgg gccagatgtg
    46561 tccaagacag gcggcgatgg caattaagcc tagccagaag gggctgacac ctaattcata
    46621 cgcgccccag acgggcaaca ttcctttcag aacgtcgaaa atcagtactg ctacggctgc
    46681 tcccttgcca ccgatacgta acacattggt tgcgcctgga ttgccggagc cgctggttcg
    46741 cggatcgggc agcccacaca agcggcaaac cagaatggca ctggaaatgg agccgcagag
    46801 gtacgcgatg aggatcattc caggcgcgat tgcactcata agctgttccg ttttgaaaat
    46861 tcgtgttcaa cgatgaatct gtggataata cgcacatttc gccggaagtg gtatccggtt
    46921 agccaaaaag caggcaggac gtgatggata ttgtatttat agagcaactt tcggtaatca
    46981 ccactattgg tgtttacgac tgggaacaga ccatcgaaca gaagttagtg ttcgatatcg
    47041 aaatggcatg ggataaccgt aaagcggcga aaagtgatga tgtggcggat tgcctcagtt
    47101 acgctgacat tgcagaaacg gtggtcagcc acgtcgaggg ggcgcgtttt gcgctggtgg
    47161 aacgcgtggc tgaagaggtg gcggagctgc tgttagcacg cttcaactcg ccgtgggtgc
    47221 gtatcaaact cagcaagcca ggcgcagtgg cgcgggcggc gaatgttggc gtaatcattg
    47281 agcgtggcaa taatctgaaa gaaaataatt aattttacag ctgttaaacc aaacggttat
    47341 aacctggtca tacgcagtag ttcggacaag cggtacattt taataattta ggggtttatt
    47401 gatgagcgat atgcactcgc tgctgatagc ggcaatattg ggtgtggtcg aaggattgac
    47461 agaatttctg ccggtatcca gcacgggcca tatgattatt gtcggtcact tgttggggtt
    47521 tgagggcgac acggcgaaaa cctttgaagt tgtgatccag ttaggatcaa ttctggcggt
    47581 agtagtgatg ttctggcggc gtctgtttgg cctgattggc atccactttg gccgcccgtt
    47641 gcagcacgaa ggtgaaagca aaggtcgttt aacgctgatc cacattttgc tggggatgat
    47701 tccggcggtg gtactggggc tgttgttcca cgacacgatt aagtcattgt ttaacccgat
    47761 aaatgtgatg tatgcgctgg tcgttggcgg tttgttgctg attgccgccg aatgcctgaa
    47821 gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc tatcgtcagg cgtttatgat
    47881 tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc cgttccgggg ccaccatttc
    47941 aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc gagttctcgt tcctgctggc
    48001 ggtaccgatg atgatgggcg caacggcgct cgatctctac aaaagctggg gcttcctgac
    48061 aaccggcgat atcccgatgt ttgccgttgg gtttatcacc gcttttgtgg tggcgctgat
    48121 agcgattaaa accttcctgc aattgattaa gcgcatttcg tttatcccgt tcgccattta
    48181 tcgctttatt gtggcggctg cggtgtacgt cgtgttcttt tgattgagaa aaggttcggg
    48241 agcaggcctg gttttctccc tcgctctgta gggagagggc cggggtgggg gtgattgttc
    48301 gcaccggtgc tggtctgttc ccctcaccct aaccctctcc ccgaaggggc gaggggatgg
    48361 ttcgtgttaa cgtttagttt tatactcagt caggctttgg gcaacgttgt tccttccaac
    48421 tggctatcgc cgcaatccgc cgtcgggtca gctcctcgcg aatctccaca cctttaaatc
    48481 ccgcttcaac gaccgctttt gtcggcactg actgcgccac ttcccaggct tcgcgcaacc
    48541 aacggccttg cgggtagtcc gctgattcaa aaccggttct gccgcgcacg tcagcctcgc
    48601 tggtcagcgc cagttgctcg acacgctgcg gtttacgcca ggcatcgatg gaatcaaaca
    48661 atttgacgat agtttttggg ttcagcattg ggaaggtgtg gatgagatcg tgaaactcag
    48721 ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg cagacgctgg cagagttgtt
    48781 ccactaattt cacacccgcc ggaccgtgac catgatgacg cggccaaagt tctggcggcg
    48841 tcagtccttt accgagatcg tggcataaag ttgcgaaacg gacatcgacc tgcggactca
    48901 gcatcgccgc cattgagagc gtcattaagg tatgaatacc cgtgtcgatt tccggatgcc
    48961 acctggcagg ggccggaacg ccaaacagtg cgtcaatttc cgggaataaa acgcgcagtg
    49021 cgccacaatc gcgcagtacc tggaagaaca cctgtggatt gcgggtggtg agggcgcttt
    49081 ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag ttcacccgca tgggtcatct
    49141 cgcgcatcag cgtcagagtt tcatcggcaa tacgaaaacc gaggtgggca taacgcgcag
    49201 caaaacgcgc cacgcgcaga acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc
    49261 gcaacagacg attttgcaga tcgcccagac cgttgtacgg gtcgataatc tcgccgttat
    49321 cgtcctgggc cagcgcatta atggtcagat cgcgacgctt aagatcatct tccagcgtga
    49381 catccggtgc ggcatagcaa gtaaaaccgg tgtaaccgga accggatttc cgttcggtac
    49441 gtgccagcgc atactcttca tgcgtttgcg gatgcagaaa cacaggaaaa tcgcggccta
    49501 cctgctggta gcccgcgtcg agcatctcct gtggcgtact gccgaccacc acccaatctc
    49561 tgtctttgac cggtagccct aacaatgcat cccgaacagc accaccgacc agataaatct
    49621 tcacgccatc atcccttcgt caaatacaca attcctaata ataagacagt gcaacgaaga
    49681 aggcgattta gttcatccag cgatctttgc gtttgcgcgt tgggatcagg tgtggcagta
    49741 ccagaccgag cagcaagcca agccccagca cgccaccgcc atacataaac cattgcatga
    49801 tgatggtgcg ctgtttgtca tccaactgta cgctggcggc atcgaccttt ttctgcgcga
    49861 caatcagctc gtttttcaat ttctgatttt cttcttttaa tccgttgatc acgctgtcgc
    49921 tctgcgccac tttttgctgc atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg
    49981 tgagtttatc ggtcagggtt ttgacctgat tttccagatc tggcacacgg gagcgcaggc
    50041 ttggctcagt gctaagttgt ttcaacggga tccaggcggt acggccagag ctgtctttca
    50101 cctgggcata attggtgttg gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa
    50161 ccgtgcccac gaggcgataa tgatctcccg gaccgctacg gacccaggta ttcagttcat
    50221 cagaaacata gcgcgtttct tcagcgtgtg agacggcagt ggcgctaagt gcgagtaaag
    50281 ttaatccgat caggcgtaat tttggcatca ggctgtcgtt attgtcatgg aaagtggaac
    50341 gatagtagtg gcatcagtgt cgctacgcaa agcattcgac atcaatcgga atcctctgtg
    50401 tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa ttgcccgttg catggcaatt
    50461 tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac
    50521 ttatgtgaca taaccgataa gtaaaggcca tggctcagga aatcgaatta aagtttattg
    50581 ttaatcacag tgccgttgag gcgttgcgtg accatctcaa tacgctgggc ggcgagcacc
    50641 atgaccccgt gcagttgctg aatatttact acgaaacgcc ggataactgg ctgcgtgggc
    50701 acgatatggg cttacgtatt cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg
    50761 caggaagagt gacaggcggc ttacaccagc gcccggaata taacgtggcg ttgagcgaac
    50821 cgacgctcga cctggcgcag ttaccgacgg aagtctggcc gaacggcgaa ttgcccgccg
    50881 atctcgcttc ccgcgtacaa ccgctgttca gcaccgattt ttatcgcgaa aaatggctgg
    50941 tggcggtcga tgacagccga attgaaatcg ctctcgatca gggggaggtg aaagcggggg
    51001 aatttgctga acctatctgt gagctggaac tggaactgct tagcggcgac acgcgcgcgg
    51061 tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt acgccagggc agcctgagca
    51121 aagcggcgcg tggctatcat ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca
    51181 ccattttgca tgttgcggca aaagccgatg tggaacaggg gctggaagcg gcgttcgagc
    51241 tggcgttagc gcaatggcag tatcatgaag aactgtgggt acgcggcaac gatgcggcga
    51301 aagagcaggt gctggcagcc attggccttg ttcgccatgc actgatgctg ttcggtggca
    51361 ttgtgccgcg taaagcgagc actcacttac gtgatttgct gactcaatgc gaagcgacca
    51421 tcgcttctgc ggtttctgcc gtgacggcgg tctactctac cgaaacggca atggcaaagc
    51481 tggcgttgac cgaatggttg gtaagcaaag cctggcagcc gtttttagat gccaaagcgc
    51541 agggtaaaat cagcgattcc ttcaaacgct ttgccgatat ccatctttcc cgccatgccg
    51601 ctgaactgaa aagcgttttc tgccagccgt taggcgatcg ctaccatgat cagttgccac
    51661 gcctgacgcg tgatattgac tcaatactgc tgctggcagg ttactatgat cctgtcgtcg
    51721 cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc tattgcgacc gggcaacgca
    51781 ttgaaattga acatttccgt aatgaggcaa acaatcagga accgttctgg ttgcacagcg
    51841 gaaaacgtta atcaggcaag gataaaatcg cttatgaagc cgctctcttc accgttacag
    51901 cagtactggc agaccgttgt tgagcggctg ccagagcctt tagccgagga atcacttagc
    51961 gcacaggcga agtcagtact tacttttagt gattttgtgc aggacagcgt gattgtgcat
    52021 ccagagtggc tgacggaact ggaaagccaa ccgccgcagg ccgacgaatg gcagcattac
    52081 gcggcatggt tgcaggaggc gctcagcaat gtgagtgacg aagccgggtt aatgcgcgag
    52141 ctgcggctat tccggcggcg cattatggtg cgcatcgcct gggcgcaaac gctggctttg
    52201 gttactgaag agagcatatt gcagcagctc agccatctgg cggagacgct gattgtcgcc
    52261 gcgcgtgact ggctgtatga cgcttgctgc cgcgagtggg gaacgccgtg caatgcgcag
    52321 ggcgaagcgc aaccgctgct gattttaggc atgggtaagc tgggcggtgg ggaactgaat
    52381 ttctcctcag atatcgatct gatttttgcc tggccggaac atggttgtac gcagggcgga
    52441 cgccgggaac tggataacgc gcagtttttt acccgcatgg ggcagcggct gattaaagtg
    52501 ttggatcaac caacgcagga tggcttcgtc tatcgcgtgg atatgcggct gcgtccgttt
    52561 ggcgaaagtg gcccgctggt gctgagcttt gccgcgttgg aggattatta ccaggagcag
    52621 gggcgcgact gggagcgtta cgcgatggtc aaggcgcgga ttatgggcga tagcgaaggc
    52681 gtctatgcta acgagttgcg tgcgatgctg cgcccgtttg ttttccgtcg ttacatcgat
    52741 ttcagcgtga ttcagtcgct gcgcaacatg aaagggatga ttgcccgtga agtgcgtcga
    52801 cgtggtttga ccgacaatat caaactcggc gcaggcggca ttcgcgaaat tgaatttatc
    52861 gttcaggtgt tccagctcat tcgcggtggg cgcgaaccgt cgctgcagtc gcactcttta
    52921 ctgccaacgc tcagcgtcat tgccgcgctg catctgcttt ctgaaaacga tgctgaacaa
    52981 ttgcgagtgg cgtatctgtt cctgcggcgt ctggaaaacc tgctgcaaag cattaacgac
    53041 gaacaaaccc agacgcttcc ttctgatgag cttaaccgtg cgcggctggc gtgggcgatg
    53101 gactttgctg actggccgca actgaccggg gcgctgaccg cacatatgac caatgtgcgc
    53161 cgggtgttta atgaattgat tggcgacgac gaaagtgaaa ctcaggaaga gtcgctgtcg
    53221 gaacagtggc gtgagctgtg gcaggatgcg ttgcaggaag atgacactac gccagtgctg
    53281 gcgcatctta gcgaggatga tcgcaaacag gtgctaacgc tgattgccga tttccgcaaa
    53341 gagctggata agcgcaccat cgggccgcga ggacgtcagg tgctcgacca tctgatgccg
    53401 catctgctaa gtgatgtctg tgcgcgtgaa gacgctgccg ttacgctgtc gcgcattacc
    53461 gccttgctgg tggggattgt tacccgcacc acctatttag aattgctcag tgaattcccc
    53521 gcggcgctta aacatttgat ttctctgtgt gccgcgtcgc cgatgattgc cagccaactg
    53581 gcgcgttatc cattattgct ggatgaattg ctcgatccaa acacccttta ccagccgacg
    53641 gcgaccgatg cctaccgcga tgagttgcgc cagtatttgc tgcgcgtgcc ggaagatgac
    53701 gaagagcaac agcttgaggc gctgcgtcag ttcaaacagg cacagctgtt acgcatcgcc
    53761 gcagcggata tcgctggtac gctaccggtg atgaaagtga gcgatcactt aacctggctg
    53821 gcagaggcta tgatagatgc cgtcgttcag caggcttggg ttcaaatggt ggcccgctac
    53881 ggtaagccaa atcacctcaa cgatcgcgaa ggccgtggtt ttgcggtggt cggctacggc
    53941 aagctgggcg gctgggagtt aggctacagt tccgatcttg acctgatctt cctccatgat
    54001 tgcccaatgg atgcgatgac tgacggcgag cgggaaatcg acgggcgaca gttttatctg
    54061 cgtctggcgc aacgcattat gcatctgttc agtacgcgta cctcttccgg cattttgtat
    54121 gaagtggatg cacggctacg cccgtccggg gccgcgggaa tgctggtgac atccgcagaa
    54181 gcatttgccg attatcagaa aaacgaggcc tggacgtggg aacatcaggc gctggtgcgt
    54241 gcgcgggtag tgtacggcga tccgcagctc accgcgcact ttgacgcggt gcgtcgcgag
    54301 attatgacgc tgccgcgtga aggtaaaact ctgcaaacgg aagtgcggga aatgcgcgag
    54361 aaaatgcgcg ctcatctcgg caataaacat cgcgatcgct ttgatatcaa agctgatgaa
    54421 ggcggaatta ccgatatcga atttattacc caatatctgg tgttgcgcta cgctcatgaa
    54481 aaaccgaagt taacgcgctg gtcagacaac gtgcgcattc tggaactact ggcgcaaaac
    54541 gacattatgg aagagcagga agcgatggcg ctgacccgtg cgtatactac gctgcgcgat
    54601 gaacttcatc atctggcatt acaggaattg ccgggccatg tgccggagga ttgcttcacc
    54661 gcagagcgtg aactggtgcg ggcaagctgg cagaagtggc tggtggaagc atgaagtatg
    54721 gtattatcgc gcgcaaattt tgaatctctc aggagacagg aatgaaagta acgctgccag
    54781 agtttgaacg tgcaggagtg atggtggttg gtgatgtgat gctggatcgt tactggtacg
    54841 gtcccaccag ccgtatctcg ccggaagcgc cggtgcccgt ggttaaagtc aataccatcg
    54901 aagaacgtcc gggcggcgcg gctaacgtgg cgatgaatat cgcttctctc ggtgctaatg
    54961 cacgcctggt cgggttgacg ggcattgacg atgcggcgcg tgcgctgagt aaatctctgg
    55021 ccgacgtcaa cgtcaaatgc gacttcgttt ctgtaccgac gcatccgact atcaccaaat
    55081 tacgggtact ttcccgcaac caacagctga tccgcctgga ttttgaagaa ggtttcgaag
    55141 gtgttgatcc gcagccgctg cacgagcgga ttaatcaggc gctgagttcg attggcgcgc
    55201 tggtgctttc tgactacgcc aaaggtgcgc tggcaagcgt acagcagatg atccaactgg
    55261 cgcgtaaagc gggcgttccg gtgctgattg atccaaaagg taccgatttt gagcgctacc
    55321 gcggcgctac gctgttaacg ccgaatctct cggaatttga agctgttgtc ggtaaatgta
    55381 agaccgaaga agagattgtt gagcgcggca tgaaactgat tgccgattac gaactctcgg
    55441 ctctgttagt gacccgttcc gaacagggta tgtcgctgct gcaaccgggt aaagcgccgc
    55501 tgcatatgcc aacccaggcg caggaagtgt atgacgttac cggtgcgggc gacacggtga
    55561 ttggcgtcct ggcggcaacg ctggcagcgg gtaattcgct ggaagaagcc tgcttctttg
    55621 ccaatgcggc ggctggcgtg gtggtcggca aactgggaac ctccacggtt tcgccgatcg
    55681 agctggaaaa tgcagtgcgt gggcgcgcag ataccggctt tggtgtgatg accgaagagg
    55741 aactgaagct tgctgtagcg gcagcgcgta aacgcggcga aaaggtggta atgaccaacg
    55801 gtgtctttga catcctgcac gccgggcacg tctcctatct ggcaaatgct cgcaaactgg
    55861 gtgaccgtct gattgtcgct gtgaacagcg atgcctccac caaacggctg aaaggggatt
    55921 cccgcccggt aaacccactc gagcagcgta tgattgtgct gggcgcactg gaagcggtcg
    55981 actgggtagt gtcgtttgaa gaagacacgc cgcagcgtct gatcgccggg atcctgccag
    56041 atctgctggt gaaaggcggc gactataaac cagaagaaat tgccgggagt aaagaagtct
    56101 gggccaacgg cggcgaagtg ctggtgctca acttcgaaga cggttgctcg accactaaca
    56161 tcatcaagaa gatccaacag gataaaaaag gctaaccgga aagcggttca cagatctcgc
    56221 ttcgtagcag gcaggcctat tgacgctgtt gttttttcaa cctaaagtaa aggaacaagg
    56281 gtaagggagg atttctcccc cctctgatca gttgttagta agtcgggaaa cttaacagta
    56341 acaacacaac cagtatgatg acgaacttca tcataaccct ttccttctat aaggcccctt
    56401 cctcgggagg ggctttcccg tttcagcccc ttgctgactc cccgattgtt gatgagcgcc
    56461 ggaccccgcg caacgctttc cctgaagcag ttttgccttc cccgagcggt cacgcaaaac
    56521 gctgcaacgt cctgagcatt tgcacctttt agcggaacgc agatataaaa aatccccggc
    56581 tttttgggcc agggatatgt gttaatgaaa ccttcttgac gctgattttt tttcaaccta
    56641 aagtaaagga acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct
    56701 gcgaaactta cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc
    56761 ttctgtaagg ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg
    56821 attgttgatg agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg
    56881 agcggtcacg caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat
    56941 ataaaaaatc cccggctttt tgtgccaggg acgttcatag ctgaaaattt attctacgtt
    57001 ttcggcgacg ggagttgttg aggttaaggg tgaagtcaat gccgccagtg agccgcctga
    57061 aacgccaatt tcattgagca aggagtcaat cagcggtgcc tgtgtgcggt aagagagagc
    57121 ggctgataat gcttgttccg ccaggtttcc gccccccaca ttacccgtgt ttgcatcacc
    57181 cgcagcgctg ccacgattca gaccatcgac ctgaataatc ttgataccgt caatagattt
    57241 catcggctca acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa
    57301 tttaaggctg gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc
    57361 ttctgcttcc gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc
    57421 ctgcatctct gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt
    57481 ttctgcatcc tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt
    57541 ctggcgagtg gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc
    57601 acgcgcttct gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg
    57661 gttggcgatt tcggtgacct gctgttgttc gatctcttta atacgaactt cacgttcagc
    57721 ctcaaccttt cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg
    57781 ttcagccaga attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat
    57841 acgtgcattc tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa
    57901 cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca tcacggtttt tctcacgcac
    57961 cgcaacttct acatcctgtt caacttcgtt acgttcgcga cgacggcgct ccgtctcctg
    58021 agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc ggattgaaat gttctttcga
    58081 ggtctggtta aagttggtaa gtgaaacgct ttccagctca agaccgtttt tcgacaggtc
    58141 ttctgcgaca gtattttgta ctccctgcac gaagttctcg cgggtatcct gtaactcatg
    58201 catggtcatt tgtgcagctg ttgcacggag ggcatcgaca aatttatctt caaccaacat
    58261 acgtaagtct tctggtgata gcgtgcgttg ccccaacgtc tgcgccgcag tggcgatgcc
    58321 ttctacagac ggttttaccc gtacaaagaa agcgactact acatcgacac gcatacgatc
    58381 tttcgtaatc aggctatcaa tggttgagcg gctgacttcc agcttcagag tattcatatt
    58441 gatggggatt atttcatgaa agatcggcat cacgattgcg ccaccgctca tcaccacttt
    58501 ttgcccacct aaaccagtac gaacaaaagc ttgctctgcc gaagcgcgac gatagagcct
    58561 ggcgaaaata attccaataa taaacagaat gcatacggca ataatcgcgg taaacatcca
    58621 ggaggacaca gaattaacaa tatcatccat tattaaattt ccttttgatt aataaattac
    58681 aatatttgtg gccagggatt gttttccgcc aaatatcgcg ttgcagaaag tcggcaaata
    58741 attaacactt tgtctccttt agtgaaaaat tttccttctt caggctccag tagtaaataa
    58801 tgtatttggc caaattgatc ggtgagtttt ccttcgcagg gattacctga agtggcttga
    58861 tgaccggtaa ttaatgccat gctaccaata tattcttctt ctgtaatagc tgaactatgg
    58921 tcgcgtggaa tccagggcgc aactatttta ccggtataat gcaccgcaat aatcgtaaac
    58981 agtaaactga caggaaccac gaacaggttc gacagtggtg attgccagac catgatgcag
    59041 gcatgctgca acaggatgcc aataagacca aaaaaaccag ccagcaagca gagaacgacg
    59101 agagctggca atcttccgat attgagataa tgaagtgcct ggctaatatg acctgtggtt
    59161 atggaatcat aatggtcaag atgtgcatca agagcaccgg agagcatgtg accgcagata
    59221 agcgctaaaa tttctaaaag gccgatcaac agtacaaagg aaatagcgaa aagataaggg
    59281 gtattatagt cggcgaataa aatcatatgt cactccaatg tccatttagt gacgcaatat
    59341 ttactttggg atagtttcct tgcttacatt
//
LOCUS       DAMZII010000014        57891 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZII010000014 DAMZII010000000
VERSION     DAMZII010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.014"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..437)
                     /gene="folK"
                     /locus_tag="POA77_000824"
     CDS             complement(<1..437)
                     /gene="folK"
                     /locus_tag="POA77_000824"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI6364300.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLR"
     gene            complement(434..1852)
                     /gene="pcnB"
                     /locus_tag="POA77_000825"
     CDS             complement(434..1852)
                     /gene="pcnB"
                     /locus_tag="POA77_000825"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDI6364301.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1891..2817)
                     /gene="gluQRS"
                     /locus_tag="POA77_000826"
     CDS             complement(1891..2817)
                     /gene="gluQRS"
                     /locus_tag="POA77_000826"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDI6364302.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2854..3309)
                     /gene="dksA"
                     /locus_tag="POA77_000827"
     CDS             complement(2854..3309)
                     /gene="dksA"
                     /locus_tag="POA77_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDI6364303.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3487..4191)
                     /gene="sfsA"
                     /locus_tag="POA77_000828"
     CDS             complement(3487..4191)
                     /gene="sfsA"
                     /locus_tag="POA77_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDI6364304.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4206..4736)
                     /gene="thpR"
                     /locus_tag="POA77_000829"
     CDS             complement(4206..4736)
                     /gene="thpR"
                     /locus_tag="POA77_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDI6364305.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDAREAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4810..7239
                     /gene="hrpB"
                     /locus_tag="POA77_000830"
     CDS             4810..7239
                     /gene="hrpB"
                     /locus_tag="POA77_000830"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDI6364306.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7435..9969
                     /gene="mrcB"
                     /locus_tag="POA77_000831"
     CDS             7435..9969
                     /gene="mrcB"
                     /locus_tag="POA77_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDI6364307.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10189..12432
                     /gene="fhuA"
                     /locus_tag="POA77_000832"
     CDS             10189..12432
                     /gene="fhuA"
                     /locus_tag="POA77_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDI6364308.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12483..13280
                     /gene="fhuC"
                     /locus_tag="POA77_000833"
     CDS             12483..13280
                     /gene="fhuC"
                     /locus_tag="POA77_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDI6364309.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13280..14170
                     /gene="fhuD"
                     /locus_tag="POA77_000834"
     CDS             13280..14170
                     /gene="fhuD"
                     /locus_tag="POA77_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDI6364310.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FILSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14167..16149
                     /gene="fhuB"
                     /locus_tag="POA77_000835"
     CDS             14167..16149
                     /gene="fhuB"
                     /locus_tag="POA77_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDI6364311.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16184..17464)
                     /gene="hemL"
                     /locus_tag="POA77_000836"
     CDS             complement(16184..17464)
                     /gene="hemL"
                     /locus_tag="POA77_000836"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6364312.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17689..19110
                     /gene="clcA"
                     /locus_tag="POA77_000837"
     CDS             17689..19110
                     /gene="clcA"
                     /locus_tag="POA77_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDI6364313.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19134..19199
                     /locus_tag="POA77_000838"
     CDS             19134..19199
                     /locus_tag="POA77_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364314.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19192..19536
                     /gene="erpA"
                     /locus_tag="POA77_000839"
     CDS             19192..19536
                     /gene="erpA"
                     /locus_tag="POA77_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDI6364315.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19583..20206)
                     /locus_tag="POA77_000840"
     CDS             complement(19583..20206)
                     /locus_tag="POA77_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDI6364316.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20244..21044)
                     /gene="btuF"
                     /locus_tag="POA77_000841"
     CDS             complement(20244..21044)
                     /gene="btuF"
                     /locus_tag="POA77_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDI6364317.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21037..21735)
                     /gene="mtnN"
                     /locus_tag="POA77_000842"
     CDS             complement(21037..21735)
                     /gene="mtnN"
                     /locus_tag="POA77_000842"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI6364318.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21819..23336
                     /gene="dgt"
                     /locus_tag="POA77_000843"
     CDS             21819..23336
                     /gene="dgt"
                     /locus_tag="POA77_000843"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDI6364319.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23466..24890
                     /gene="degP"
                     /locus_tag="POA77_000844"
     CDS             23466..24890
                     /gene="degP"
                     /locus_tag="POA77_000844"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDI6364320.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25045..26202
                     /gene="cdaR"
                     /locus_tag="POA77_000845"
     CDS             25045..26202
                     /gene="cdaR"
                     /locus_tag="POA77_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDI6364321.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26291..26677)
                     /locus_tag="POA77_000846"
     CDS             complement(26291..26677)
                     /locus_tag="POA77_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDI6364322.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26992..27816)
                     /gene="dapD"
                     /locus_tag="POA77_000847"
     CDS             complement(26992..27816)
                     /gene="dapD"
                     /locus_tag="POA77_000847"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDI6364323.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27847..30519)
                     /gene="glnD"
                     /locus_tag="POA77_000848"
     CDS             complement(27847..30519)
                     /gene="glnD"
                     /locus_tag="POA77_000848"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDI6364324.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30581..31375)
                     /gene="map"
                     /locus_tag="POA77_000849"
     CDS             complement(30581..31375)
                     /gene="map"
                     /locus_tag="POA77_000849"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI6364325.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31743..32468
                     /gene="rpsB"
                     /locus_tag="POA77_000850"
     CDS             31743..32468
                     /gene="rpsB"
                     /locus_tag="POA77_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI6364326.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32726..33577
                     /gene="tsf"
                     /locus_tag="POA77_000851"
     CDS             32726..33577
                     /gene="tsf"
                     /locus_tag="POA77_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI6364327.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33724..34449
                     /gene="pyrH"
                     /locus_tag="POA77_000852"
     CDS             33724..34449
                     /gene="pyrH"
                     /locus_tag="POA77_000852"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI6364328.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34741..35298
                     /gene="frr"
                     /locus_tag="POA77_000853"
     CDS             34741..35298
                     /gene="frr"
                     /locus_tag="POA77_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI6364329.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35390..36586
                     /gene="ispC"
                     /locus_tag="POA77_000854"
     CDS             35390..36586
                     /gene="ispC"
                     /locus_tag="POA77_000854"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDI6364330.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36775..37533
                     /gene="ispU"
                     /locus_tag="POA77_000855"
                     /gene_synonym="uppS"
     CDS             36775..37533
                     /gene="ispU"
                     /locus_tag="POA77_000855"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDI6364331.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37546..38403
                     /gene="cdsA"
                     /locus_tag="POA77_000856"
     CDS             37546..38403
                     /gene="cdsA"
                     /locus_tag="POA77_000856"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6364332.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38415..39767
                     /gene="rseP"
                     /locus_tag="POA77_000857"
     CDS             38415..39767
                     /gene="rseP"
                     /locus_tag="POA77_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDI6364333.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39797..42229
                     /gene="bamA"
                     /locus_tag="POA77_000858"
     CDS             39797..42229
                     /gene="bamA"
                     /locus_tag="POA77_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDI6364334.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42351..42836
                     /gene="skp"
                     /locus_tag="POA77_000859"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42351..42836
                     /gene="skp"
                     /locus_tag="POA77_000859"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDI6364335.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42840..43865
                     /gene="lpxD"
                     /locus_tag="POA77_000860"
     CDS             42840..43865
                     /gene="lpxD"
                     /locus_tag="POA77_000860"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDI6364336.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43970..44425
                     /gene="fabZ"
                     /locus_tag="POA77_000861"
     CDS             43970..44425
                     /gene="fabZ"
                     /locus_tag="POA77_000861"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI6364337.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44429..45217
                     /gene="lpxA"
                     /locus_tag="POA77_000862"
     CDS             44429..45217
                     /gene="lpxA"
                     /locus_tag="POA77_000862"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDI6364338.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45217..46365
                     /gene="lpxB"
                     /locus_tag="POA77_000863"
     CDS             45217..46365
                     /gene="lpxB"
                     /locus_tag="POA77_000863"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDI6364339.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46362..46958
                     /gene="rnhB"
                     /locus_tag="POA77_000864"
     CDS             46362..46958
                     /gene="rnhB"
                     /locus_tag="POA77_000864"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6364340.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46995..50477
                     /gene="dnaE"
                     /locus_tag="POA77_000865"
     CDS             46995..50477
                     /gene="dnaE"
                     /locus_tag="POA77_000865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6364341.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50490..51449
                     /gene="accA"
                     /locus_tag="POA77_000866"
     CDS             50490..51449
                     /gene="accA"
                     /locus_tag="POA77_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDI6364342.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51548..53689
                     /gene="ldcC"
                     /locus_tag="POA77_000867"
     CDS             51548..53689
                     /gene="ldcC"
                     /locus_tag="POA77_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDI6364343.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYAMGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53746..54135
                     /locus_tag="POA77_000868"
     CDS             53746..54135
                     /locus_tag="POA77_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6364344.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54200..55498
                     /gene="tilS"
                     /locus_tag="POA77_000869"
     CDS             54200..55498
                     /gene="tilS"
                     /locus_tag="POA77_000869"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI6364345.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55547..55807)
                     /gene="rof"
                     /locus_tag="POA77_000870"
     CDS             complement(55547..55807)
                     /gene="rof"
                     /locus_tag="POA77_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDI6364346.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55794..55994)
                     /locus_tag="POA77_000871"
     CDS             complement(55794..55994)
                     /locus_tag="POA77_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDI6364347.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56160..56705
                     /locus_tag="POA77_000872"
     CDS             56160..56705
                     /locus_tag="POA77_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDI6364348.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56702..57124
                     /gene="arfB"
                     /locus_tag="POA77_000873"
     CDS             56702..57124
                     /gene="arfB"
                     /locus_tag="POA77_000873"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6364349.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57138..57848
                     /gene="nlpE"
                     /locus_tag="POA77_000874"
                     /gene_synonym="cutF"
     CDS             57138..57848
                     /gene="nlpE"
                     /locus_tag="POA77_000874"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDI6364350.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13590 a        14355 c        16118 g        13828 t
ORIGIN      
        1 tcacgcaacg tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac
       61 agcataaatc cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc
      121 acttcattac caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct
      181 ttacgaacac gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg
      241 gcgagcgaag tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc
      301 agcggtgggg tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct
      361 aatgctttca gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca
      421 atatacgcca ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag
      481 tacggcgacg cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg
      541 gcgcggaaac ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt
      601 caacttcagc tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca
      661 gtttccatgc gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc
      721 gggttaacgt cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt
      781 cgttcatcgc cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcaatcttct
      841 gtgccgtctc cagcagtggg taccagaaca tggcggcaaa caggaacgcc gggttcacgc
      901 gcatatcgtt atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca
      961 tcgggctgtc gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca
     1021 gatgatattc acacaacagc ttataggtat cgtaaccgta gcccgcttgt agcagtttaa
     1081 gcgattcttc aaacaggcgt gccggtggga tatcgttcaa cagggtagcg aggcgaggga
     1141 tcggttctgc ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc
     1201 gcagcatacg taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa
     1261 cgccgtcctt cagatccttc ataccgccaa cgtaatcacg gacggtaaaa tccgctacgc
     1321 tgtaatagag gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga
     1381 aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga
     1441 cgttaccttc gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatta
     1501 catgagccag acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct
     1561 gctcaggcgt ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt
     1621 cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta
     1681 ccttcagggc attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca
     1741 ccgtcacctg tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct
     1801 tgcggcaaaa attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt
     1861 caggacaaaa aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt
     1921 tacaattgcc gactcgggca cggcggtaag ccgccagttt ttgacggctg actgaaggat
     1981 ttgctcgacg ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc
     2041 cgcgattagt accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga
     2101 aagtttagcg ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca
     2161 gccaaaaagc tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg
     2221 cactatttct gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa
     2281 caacccatca cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat
     2341 aatgccgcgc agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc
     2401 ggcattgtct ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct
     2461 ttgaatacgc gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc
     2521 gagtgcttca cgataggcgt ggtgacgttg cgattgccag agaacatcgc cgtcccagtg
     2581 cagaccgtaa tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg
     2641 cgggtcgata tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata
     2701 gctgccgagc gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa
     2761 gcggccaata tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc
     2821 ccgcctgtgg taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat
     2881 ttcagccagc gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg
     2941 aataccaatt tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt
     3001 cagcgtcttc tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag
     3061 gctgaactct tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc
     3121 ctgcatatgt gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag
     3181 aatacgacgg aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg
     3241 atatggttcc accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc
     3301 ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg
     3361 aggccgctat aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac
     3421 aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt
     3481 actttactac aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc
     3541 cgctttgtaa gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta
     3601 tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa
     3661 aaagataacc gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg
     3721 acctcgttca gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga
     3781 tttcacttca atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct
     3841 gcgttctgcg ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat
     3901 tgattcatta aggatagcct ctttcgtcaa cctgttagcc caaagcgtgt tgacgcaaat
     3961 aaatgcgccg ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt
     4021 gtctgaagtc gaataccaga cggtatcgcc aggcgttgca caaccggtca ttgcacccgt
     4081 attcgggcag tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg
     4141 tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga gagaattcca ttgcgacaat
     4201 ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca
     4261 aacgaggagg cgtaaagggt gaactccgtc accgcatacg accagttaaa acctggcggc
     4321 gggattgtca ccgcctctct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga
     4381 ttgctttgaa aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt
     4441 aagccgcgtg gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg
     4501 gcgtcatcaa gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc
     4561 gccttctctt tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg
     4621 gcggcgaccg gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt
     4681 tcgcggattt ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga
     4741 ggcactcggt tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg
     4801 gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc
     4861 gattgtgcgc cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg
     4921 ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt
     4981 cgtctggcgg cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc
     5041 gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa
     5101 gtggttaccg aaggcgtgct gacgcgcatg atccagcgtg acccggaact gagcggtgtt
     5161 ggactggtga tccttgatga atttcatgag cgcagcttgc aggcggattt ggcgttggcg
     5221 ctgttactcg atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct
     5281 accctggaca acgaccgctt gcagcaaatg ctgccagaag cgcctgtcgt catctcagaa
     5341 gggcgctcgt ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac
     5401 gatgccgttg cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta
     5461 tttttacctg gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc
     5521 agtgatgtat tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg
     5581 atcctcccgg caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc
     5641 agtttaacca ttgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt
     5701 tttgatccgc gcacggggct tacgcgactg attactcaac gcgttagcca ggcatccatg
     5761 acgcagcgtg ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tttaatcgcc
     5821 aaggaacaag cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt
     5881 tccggtttgc tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg
     5941 ctggatcaac cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg
     6001 gcgctggagg gtgaacgact tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat
     6061 ccgcgtttag cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca
     6121 aaaattgccg ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg
     6181 ttttcgcgca atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac
     6241 gtacgtggcg gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc
     6301 gatcgcattg ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga
     6361 gcgatgctcg atgccaacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta
     6421 ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg cgctgctggt cgatattgat
     6481 gagttagtac aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac
     6541 gcgcaaggta cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg
     6601 cagccgctgg cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt
     6661 gataaaggtt taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg
     6721 ttatgcgccg caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta
     6781 ttggcagcgc tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc
     6841 ctgaaatcac tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa
     6901 cgtctggata gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt
     6961 cgttatcatg aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag
     7021 gccaccaatc cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct
     7081 gcccaaaggc cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt
     7141 gaggtgcaaa aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca
     7201 aatactgcac cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc
     7261 tgtgggagag ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct
     7321 ccctcaaggg agaggggacc gatcgagcac aaattttgag agatatcttc ttctgtcttg
     7381 taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc
     7441 gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag
     7501 gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat
     7561 gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa
     7621 cgcggctggc tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt
     7681 tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg
     7741 cctgcggcag tttatggccg aatggtcaat cttgagccag acatgaccat cagcaagaac
     7801 gagatggtga agctgctgga ggcgacccag tatcgtcagg tgtcgaaaat gacccgtcct
     7861 ggcgaattta ccgtgcaggc caacagcatt gagatgattc gccgtccgtt tgatttcccg
     7921 gacagtaaag aaggacaggt gcgcgcgcgt ctgacctttg atggcgatca tctggcgacg
     7981 atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc
     8041 accatgatct cttcgccaaa cggtgagcag cgtctgtttg tgccgcgcag tggtttcccg
     8101 gatttgctgg tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga
     8161 atcagtctct actcaatcgg acgtgcggtg ctggcaaacc tgaccgccgg acgcacggta
     8221 cagggtgcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt
     8281 tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc
     8341 aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac
     8401 gaaatccgcg gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta
     8461 agcctcgacc agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg
     8521 tggcgtaacc caaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag
     8581 caacagatta ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag
     8641 ccgcgcggtg gggtgatctc tcctcagcca gcctttatgc aactggtgcg tcaggagctg
     8701 caggcaaaac tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt
     8761 gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag
     8821 aaacagcgta agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt
     8881 gaagttcgtg cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg
     8941 caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttacctgac ggctttaagc
     9001 cagccgaaaa tctatcgtct gaatacgtgg attgcggatg cgccaatcgc gctgcgtcag
     9061 ccgaatggcc aggtctggtc accgcagaac gatgatcgtc gttatagcga aagtggcaga
     9121 gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa tctggggatg
     9181 gcgctggggc tgcctgcggt tacggagacc tggatcaaac tgggcgtgcc gaaagatcag
     9241 ttgcatccgg ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg
     9301 caggcattcc agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg
     9361 gtaatcgcgg aagatggcaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt
     9421 ccggcgcagg cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg
     9481 ggtcgtcaac ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc
     9541 aacaataacg tagatacctg gtttgcgggc attgacggca gcacggtgac catcacctgg
     9601 gtaggccgtg ataacaacca gccgaccaaa ctgtatggtg ccagcggggc aatgtcgatt
     9661 tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat
     9721 attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc
     9781 ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagtgagat gcagcagcaa
     9841 ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct
     9901 gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt
     9961 agtaattaac atctaagcgt gaaataccgg atggcgaatt gccatccggt aaaataacat
    10021 cccatctaag atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc
    10081 tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat
    10141 aatcattctc gtttacgtta tcattcactt tacatcagag atataccaat ggcgcgttcc
    10201 aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggta
    10261 agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt
    10321 accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcga
    10381 cagtctgcta ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct
    10441 gttgtgaccg ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc
    10501 tacacgccgg gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc
    10561 attcgcggct ttgcggcaga aggccaaagc cagaataact atctgaatgg cctgaagttg
    10621 cagggcaact tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt
    10681 atgcgtggcc cggtttccgt gctttacggt aaaagcagtc ctggcggcct gttgaatatg
    10741 gtcagcaagc gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac
    10801 agcctgttcc agactggttt tgactttagc gattcgttgg atgatgacgg tgtttactct
    10861 tatcgcctga ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag
    10921 cgttatgcta ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa ttttaccttc
    10981 ctttcttact tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga
    11041 accgttgagc cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag
    11101 aacaacacct attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac
    11161 gacaccttta ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc
    11221 gtttatggtt acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca
    11281 ttagcgccag cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag
    11341 ctgcaaaact tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac
    11401 cacaccctgc tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt
    11461 ggttacgacg actctgtgcc actgctcaat ctgtacaatc cggtgaatac cgatttcgac
    11521 ttcaatgcca aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa
    11581 acgggcgttt atgttcagga tcaggcgcag tgggataaag tgctggtcac cctgggcggt
    11641 cgttatgact gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt
    11701 gatgacaaac agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca
    11761 ccttacttca gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat
    11821 attttcgcac cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat
    11881 cgtccgattg tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg
    11941 gaccctgagg gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa
    12001 atcgaagcga aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc
    12061 gatgcggaat acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa
    12121 cacatggctt cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg
    12181 ctgggcaccg gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa
    12241 gtgggaagtt atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg
    12301 gcgggctcca acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc
    12361 tgctttaaca cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc
    12421 ttccgtttct aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg
    12481 ttatgcagga atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc
    12541 gtgtgcccgg gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga
    12601 ccggtctgat tggtcacaac ggttctggta aatccactct gctcaaaatg cttggccgtc
    12661 atcagcctcc gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca
    12721 gcaaagcgtt tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga
    12781 tgaccgtgcg tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct
    12841 ttggtgcggc agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc
    12901 tggcgcatcg gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca
    12961 tgctggtggc gcaggatagc cgttgtctgt tgctcgacga accgacttcg gcgctggata
    13021 tcgcccacca ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga
    13081 cggtcattgc cgtgctgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg
    13141 ccctgcgcgg tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa
    13201 ccctcgaaat gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac
    13261 ctgtgagttt tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac
    13321 ggcgatggcg ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga
    13381 ccccaatcgt attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat
    13441 cgtgccttac ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt
    13501 gccggactca gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga
    13561 aatgaaaccg tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc
    13621 acgtattgcg ccgggtcgcg gatttaactt cagtgacggc aaacagccgt tggcgatggc
    13681 gcgtaaatcg ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca
    13741 tttagcgcaa tacgaagact ttatcctcag catgaaaccc cgctttgtga agcgtggtgc
    13801 acgcccgtta ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa
    13861 cagcttgttc caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac
    13921 caacttctgg ggcagtaccg ccgtcagtat cgatcgtctg gcggcgtata aagacgttga
    13981 tgtgctctgt tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct
    14041 gtggcaggcc atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt
    14101 ttatggtgca acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg
    14161 taaagcgtga gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt
    14221 gtcgctacgg cgctaacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg
    14281 caggccgcct ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg
    14341 ttgccgcgtc tggcgatttc gctgctggtg ggcgcaggcc tggggctggt gggcgtgctg
    14401 tttcagcaag tgctgcgtaa tccgctggcg gaaccgacga cacttggcgt tgctacaggc
    14461 gcgcaactgg gaattaccgt caccacactt tgggcgatcc caggtgctat ggcgagccag
    14521 tttgccgcac tggtgggcgc ttgtgtcgtt ggcttaatcg tctttggcgt cgcgtggggg
    14581 aaacggcttt cgccggttac gcttattctc gcggggctgg tggtgagcct ttattgtggg
    14641 gcaattaatc agctactggt tatcttccat cacgaccagc tgcaaagcat gttcctgtgg
    14701 agcaccggaa cgctgacgca aaccgactgg ggcggcgttg agcgtttatg gccgcagttg
    14761 ctgggcggcg tgatgttgac gttattgcta cttcgcccgt taaccctgat ggggcttgat
    14821 gatggcgtgg cgcgcaatct cgggctggcc ttgtcgctcg cgcgtctggc agcgctgtcg
    14881 ctggcgattg tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg
    14941 ttgttcgcgc cactgctggc aaaaatgctg ggggcgcggc gtttattgcc acggttgatg
    15001 ctggcgtcgc tgattggtgc gttaattctg tggctttctg atcaaatcat cctctggctg
    15061 actcgcgtgt ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg
    15121 ctactgtggc tgctgccgcg tttacggagc attagcgcgc cggatatgaa ggtcaacgat
    15181 cgtgtcgcga ctgaacgcca acatgtgctg gcgtttgtcc tcgcgggcgg cgtgctgctg
    15241 ttgatggctg tggtagtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg
    15301 agcggggcgt tgcttgagga tttgatgccc tggcgctggc cgcgaattat ggcggcgctg
    15361 tttgcgggcg tcatgctggc ggtggcgggc tgtattattc agcggctgac cggaaacccg
    15421 atggcaagcc cggaagtgct ggggattagc tctggcgcag cgtttggcgt ggtgttgatg
    15481 ctgtttctgg tgccgggtaa tgcctttggc tggctgttac ctgcaggcag tctcggggcg
    15541 gcggtgacgc tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc
    15601 atgttactgg cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag
    15661 gcaagtggtg acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac
    15721 gcgaccgatg cgcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattacc
    15781 ccgctgtgcc gccgctggct gaccatttta ccgctgggtg gtgataccgc ccgagccgta
    15841 ggaatggcgc taacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg
    15901 accgcgacga tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg
    15961 cggatgatgg gctttcgacg gacgatgccg cacatcgtaa tttcggcgct ggtgggtggt
    16021 ttactgctgg tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg
    16081 gcggggctgc tgtcaacctt tatcggcgcg ccatatttta tctatttgtt gagaaagcag
    16141 agccgttaat tgaatgcctc gcccggtggg cgaggcaaaa tcatcacaac ttcgcaaaca
    16201 cccgacgtgc agcatcgatg gtgttattga tatcttccat gctgtgcgcc acggacataa
    16261 agcccgcttc aaacgctgat ggtgccaggt aaacaccttc atccagcatc atatggaaga
    16321 aacgcttaaa gcgttccacg tcacaggcca tcacatcctg atagcacgtc acggactcgg
    16381 cgtcggtaaa gaaaataccg aacatgccgc caacgtggtt aacgaccagc ggaattccgg
    16441 cttcttctgc cgcttccagc agaccttctg ccagacgtga tgtcagctca tccaacgttt
    16501 cgtgaacgcc cggctgcgcg acttcattca gacaggcgaa accggctgcc atcgcaattg
    16561 ggttaccgga aagcgtaccc gcctgataga ccggacccgt cggggccagc gcatccatta
    16621 catcacgacg accaccgaat gcgcctaccg gcattccacc gccgatgatt ttgccgaggc
    16681 aggttaaatc tggcactacg ccgtaataat cctgtgcacc agccagtgcc acgcggaagc
    16741 cagtcattac ttcatcgatg atcagcaatg cgccaaattc gtcgcacagc gcgcgcagac
    16801 ctggcaggaa ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcaacga
    16861 taatacaggc aatctcttgc gggtattgct caaacgcggc gcgtacagaa gccagatcgt
    16921 tataagtaca ggttaaggta tgtttggcga aatctgccgg aacacccggc gagtttggct
    16981 ggcctaacgt gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt
    17041 aacaaccttc aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg
    17101 cgctcatcgt cgcttcagtg ccggagttca ccatgcgcac catatccatg gtcgggacca
    17161 gttcggtcac cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac
    17221 cacgctcggc ggcttcaatc acggcattgc ggatagccgg atggttatgg cccagcacca
    17281 tcggccccca ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag
    17341 cgccgtccgc tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag
    17401 gggagttcac accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac
    17461 tcatggaggg ttcctgattc gtagaaaaag tgaatggctg ctattctatg ttattcataa
    17521 caagttaaat actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga
    17581 gtataaaagt tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc
    17641 gagtaaaatg ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat
    17701 actccctctt tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa
    17761 cttcttgagc gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg
    17821 cttgtcgggc tggcagcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt
    17881 atgggggcgc tggtacatac tgctgataat tatccgcttc tgttaaccgt cgcttttctc
    17941 tgttcggcgg tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca
    18001 gggggatcgg ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg
    18061 tggcgtgtat tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatggtg
    18121 ctagggcgcg aaggaccaac cgtgcagatc ggcggcaata tcggtcgcat ggtgcttgat
    18181 attttccgcc tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg
    18241 gggctggctg cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg
    18301 cgtccgcagt ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg
    18361 tcgaccatta tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt
    18421 tctgatgcgc cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt
    18481 ttcggcccta tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac
    18541 ggcggcaata ttaccaaatg ggtactgatg ggtggtgcga ttggcggtct gtgtggattg
    18601 ctggggtttg tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc
    18661 gcggggaatt tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc
    18721 ttactctgct tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt
    18781 actgtgctgg gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg
    18841 gaggcgggga cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg
    18901 ccgttaacgg ggatcattct ggttctggag atgaccgata actaccagct cattttgcca
    18961 atgattatta ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaaccgcta
    19021 tactcggcga ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc
    19081 aaggctgcat cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga
    19141 ttattattgg gttagaattt gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat
    19201 gacgtagcac tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc
    19261 gctgacgaag ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc
    19321 ggcttccagt atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa
    19381 aaacagggcg ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc
    19441 gttgattata ccgaaggtct ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa
    19501 agcacctgcg gttgcggttc ttcctttagt atctaatctg ttgttcatgt gccggataaa
    19561 ctttccggca catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct
    19621 taagatgcca acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac
    19681 tggctgtttc cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga
    19741 taatacaggc agttgcgtag atttctgtac gtaaaatcat ggggatttcg cgggccagaa
    19801 catcacgaat gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg
    19861 gaccggcttc cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa
    19921 cggcgtccag caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga
    19981 tggtcagcat gctggttacc attgcaacga ccagatcggt gggatctttc acccaaaata
    20041 ccgggccgtg atccagcgcc atgtcgcgaa ttgtcccgcc gcctacagcg gtaaccacgc
    20101 ccagtaccag aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccggaga
    20161 tggcaaatac cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac
    20221 tgagcacctg aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt
    20281 gcagcgagga taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg
    20341 ggaattttga gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct
    20401 gtaatgacaa tcgcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag
    20461 ggaacccggc tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga
    20521 atcgactctt ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt
    20581 ttaggtttat cagcatattg cgctttcaat tgcgcgtact gatccagcag ggattgcgcg
    20641 gcttgttcgg ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca
    20701 atttgttcaa tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg
    20761 tcaacctgcc gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg
    20821 acaatgcgtt ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt
    20881 ggaggatagt cggaatagct gctgacccca accggcgtga tcccggcagc aaaggcaagt
    20941 tcagtgttgg cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgca
    21001 agaaaagaca gggcgaccag cgccctgaac agtgacttag ccatgtgcaa gtttctgcac
    21061 cagtgactca accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct
    21121 aagatgagac tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt
    21181 gaaattgtgg cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg
    21241 gaagttgtgg cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac
    21301 aatcaggcca cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat
    21361 cagtttatcg tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc
    21421 aaatgccgtg acatccgcgt cgtgataacg tgcttcgtcc gagacaacga tatcgcccac
    21481 tttcagcgtt ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca
    21541 gtgttccaac agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag
    21601 aagcgcaacc tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat
    21661 agtttgacgg ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat
    21721 gatgccgatt ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg
    21781 cgcttacggg gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa
    21841 aaaaaataaa ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga
    21901 tcctgcggat cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc
    21961 tgcaacaaaa gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta
    22021 ctcactcgat ggaagtccag caggtggggc gctacatcgc caaagaaatt ttaagccgtc
    22081 tgaaagagct taaattgctg gaagcatacg gcctggatga actgaccgga ccttttgaaa
    22141 gcattgttga gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg
    22201 gcgaagcagc gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc
    22261 agcctctgac tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac
    22321 cgcttaacga gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac
    22381 aaggcattcg tctggtgcat acattgatgc ggatgaatct cacctgggca caggttggcg
    22441 gtattttaaa atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact
    22501 atttaatgaa aaagccgggt tattatcttt ctgaagaagc ctatattgcc cggttgcgta
    22561 aagaacttaa tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg
    22621 acgacatctc ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg
    22681 ttgagcagct ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct
    22741 tttcgctggt ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta
    22801 cggaagatca gttttttatg tatttacgag taaataccct aaataaactg gtaccctatg
    22861 cggcacaacg atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat
    22921 tattggaaga tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa
    22981 aacatgtgtt tagccatcca gatgtcgagc agcttgaatt gcagggctat cgggtcatta
    23041 gcggattatt agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac
    23101 tggtagaaaa agaacgggtg aaacgtttcc ctattgaatc gcgcttattc cacaaactct
    23161 cgacgcgcca tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg
    23221 agtttccgct atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta
    23281 tgaccgacct ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag
    23341 gcttttgtaa agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc
    23401 gcgctataaa acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga
    23461 aatacatgaa aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt
    23521 tatctccgct ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc
    23581 caagccttgc accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag
    23641 gtagcacaac cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg
    23701 attctccgtt ctgccaggaa ggttctccgt tccagagctc tccgttctgc cagggtggcc
    23761 agggcggtaa tggtggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca
    23821 ttgatgccga taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtca
    23881 ttaaagttca actgagcgat ggccgtaagt tcgacgcgaa gatggttggc aaagatccgc
    23941 gctctgatat cgcgctgatc caaatccaga acccgaaaaa cctgaccgca attaagatgg
    24001 cggattctga tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc
    24061 tgggcgagac ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgccg
    24121 aaaactacga aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg
    24181 cgctggttaa cctgaacggt gaactgatcg gtatcaacac cgcgatcctc gcaccggacg
    24241 gcggcaacat cggtatcggt tttgctatcc cgagcaacat ggtgaaaaac ctgacctcgc
    24301 agatggtgga atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga
    24361 actccgaact ggccaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg
    24421 ttctgcctaa ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac
    24481 tgaacggtaa gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg
    24541 tgggcagtaa actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg
    24601 aactgcagca gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag
    24661 gcgctgagat gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaaaacgg
    24721 gcactccggc tgcgcagatc ggcctgaaga aaggtgatgt gattattggc gcgaaccagc
    24781 aggcagtgaa aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg
    24841 cactgaacat tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa
    24901 ccccttcctg aaaacgggaa ggggttctcc ttacaatctg tgaacttcac cacaactcca
    24961 tacatcttca tcatccttta ggcatttgca caatgccgta cgttacgtac ttccttatgc
    25021 taagccgtgc ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag
    25081 gatatcgtgg cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgt
    25141 gggcgaatta tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg
    25201 ctggtacttt cacagggacg agtcgtcgat atcgatgacg cggtggcacg tcatctgcac
    25261 ggtgtgcggc aagggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt
    25321 ggcctgacag gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct
    25381 gaaatgatgc tggaacagtc gcggttgatg cacctgttgg cgcaggatag ccgtttgcgg
    25441 gaagaactgg tgatgaacct gattcaggct gaggagaata ctcccgcact tactgaatgg
    25501 gcgcaacggc tggggatcga tctcaatcaa ccgcgagtag tggctattgt tgaggtcgac
    25561 agcggtcagc ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaatgcgctg
    25621 actacgcccg agcgtaataa tctggtggcg attgtctcgc tgaccgaaat ggtggtgttg
    25681 aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa
    25741 caactgatta cccgcatgaa agagtatggc cagctgcgtt ttcgcgtttc gctgggcaac
    25801 tattttaccg gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg
    25861 gtgggtaaac agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct
    25921 gtgttactcg acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg
    25981 cggctgaaag cgatggacaa taatggcttg ctgcgacgaa cgctggcggc gtggtttcgc
    26041 cacaatgtgc aaccgctggc tacgtcaaag gcgctgttta ttcatcgtaa tactctggag
    26101 tatcggctta atcgtatatc ggaactgacc gggcttgatt tgggtaattt tgatgacagg
    26161 ttgctgctgt atgtggcgtt gcagctggat gaagagcggt aggttatgcg ttaaggtcga
    26221 ggcggcgctg gctcattccc ctcagcccga agggtagagg ggaatgtccg agttaaccgt
    26281 cagttcggca ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat
    26341 tggttacgac tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt
    26401 cgctgcggtc acgctggcag atttgatcaa gctcatcaat gatataccgt agattcgggc
    26461 tgatttcctg gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct
    26521 gacgcggata tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat
    26581 agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag
    26641 gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag
    26701 taaattgtgc ttattttagc atttggcctg gcccgcccgg ctcgactgtg atcgcagaaa
    26761 gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt
    26821 ggcgatattc catgggatgg attaacacat ttatcttcgt tttgcgaatt acatgacagt
    26881 ccgaaacaag atgaaaaagt atttaaaaaa ggggggaaga agaatgagta ggacgaatta
    26941 ttctgagcca gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat
    27001 ggtacgcagc agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac
    27061 gataaccgca cagtagaggc tgtatttgcc atctttcgac ggcagattac ctgaaacaac
    27121 cacagacccc gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg
    27181 ggtgctctga ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc
    27241 ttcaaccact tcggagcgtg cgccaatgaa gcagttatct tcaataatgg tcgggttagc
    27301 ctgcagcggc tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc
    27361 aatctgcgca caagaaccga cggtcgccca ggtatcaacc atcgtgcctt catcaacata
    27421 tgcgccgatg ttgacgtaag acggcatcag cacggtgtta cgggcaataa atgcaccctg
    27481 acgtaccgcc gctggtggca caacgcggaa gccttctttc tgaaaacgcg cttcgtcgta
    27541 gtcggcgaat ttcatcggca ctttatcgaa gtagcggctt tctgcccctt cgatcacctg
    27601 attatcatta atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca
    27661 ctgaccgtca attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg
    27721 atttaccgct tcgcgggtta cggtgtctgc attggctggc gtgatctcgg cacggcgttc
    27781 aaaagcggtt tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta
    27841 aacacatcac cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg
    27901 ctgcaactcg ttattaagtg cacgccggtc agcggtggca attatgaata aatcttctac
    27961 tcgctcgcca atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat
    28021 tttcccgact ctcgccagca gtcctggctg gtcgagcgcg ataagctcaa ggaacgattt
    28081 gcggtcggta tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt
    28141 ggcgggttgg cgacggggct gcggtggctg ccagctactt tgcgtcagta cttgctccag
    28201 accaaaccga ataacctcat gacgatctgc ggacagcggg ctgccatcgg gttccagcac
    28261 gataaaggta tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact
    28321 taaattgcgg cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct
    28381 ccagataaaa atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt
    28441 gcttaaatca tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg
    28501 gacaaaatag ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt
    28561 atccatgcgc agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc
    28621 cggcgtgttt tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa
    28681 cagactttgc ttccagctat tccacagcgt ttcgttggtg gcgcaaatgt cagccactgt
    28741 caggcatacc agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat
    28801 gacttcaggg tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg
    28861 aaccagccag gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg
    28921 cactacatcc tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg
    28981 aaacagcgcg gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca
    29041 caacggatgg cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg
    29101 gatagtatgt tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat
    29161 atgcgaccat tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagccc
    29221 gcgccgcacc gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttccggaat
    29281 attacacagc ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga
    29341 gtaaatgccg gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc
    29401 cggctggcgc ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa
    29461 ctcatcgtcg attggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa
    29521 cagttgcagc agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt
    29581 catcatccgc tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct
    29641 aagttggcga tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa
    29701 gcgaatacgc cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa
    29761 gaagccaaac ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca
    29821 ttgcagagtg tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt
    29881 gtagctggtg ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgccgcgta
    29941 gaacttgtcg gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag
    30001 cgcaacatcg ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga
    30061 taacccttcc agcatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac
    30121 atcccagagc agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt
    30181 acggcttaaa atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc
    30241 accgacggcg accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg
    30301 ttgcaggagc tggtcgataa actcggtgcg cgcctcaatc aactgttctg cagagatccc
    30361 attgtcaaag gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccacc
    30421 gacggttaat tcatcacggg gccagacgca tggattttgc ggttgaccgg gcagggtggg
    30481 gagagcggtg tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta
    30541 aattatcgca ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta
    30601 tcgccgggat ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca
    30661 caatagtatg ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat
    30721 ctttcatggt gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca
    30781 tccccggttt cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt
    30841 catggaagcc gcgaccaata ccgtgtccac aatattcacg aacgacggag aagccttctg
    30901 cttcgacaaa cttctgaatc gccgcaccga tttcgcgcag gttaatgcct ggtttgacca
    30961 tgcgcagcgc caggtacagg ctttcttgcg tgatgcggca cagacgttcg cccatgatgg
    31021 tcggcttacc gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg
    31081 tgacatcaat gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt
    31141 ggcacaccac ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc
    31201 aggcagaaac cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc
    31261 cggtgctgac gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc
    31321 ggccagcgac gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt
    31381 ctgtccatca gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca
    31441 cccgggcaaa ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata
    31501 atagaaaatt cgacatctga tgctgtacac agcgccaaca attattggtg tccatgacgt
    31561 atttgtggta taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa
    31621 gcgacaatct cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt
    31681 tggggtcggt aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa
    31741 tcatggcaac tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga
    31801 cccgttactg gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca
    31861 tcatcaacct tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga
    31921 ttgcttctcg caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg
    31981 tgaaagacgc tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta
    32041 tgctgactaa ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc
    32101 agtctcagga cggtactttc gaaaagctga ccaagaaaga agcgctgatg cgcactcgtg
    32161 agctggagaa actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg
    32221 ctctgtttgt aatcgatgct gaccacgaac acattgctat caaagaagca aacaacctgg
    32281 gtattccggt atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta
    32341 tcccgggtaa cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa
    32401 ccgtacgtga aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag
    32461 ctgagtaata aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc
    32521 tcgagaattc ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc
    32581 atctgcggcg cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt
    32641 tagggggcct gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc
    32701 agatcacatc tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct
    32761 gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg
    32821 cgacatcgag ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa
    32881 agcaggcaac gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat
    32941 cattctggaa gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt
    33001 cgcagacaaa gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa
    33061 agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa attggtgaga acatcaacat
    33121 tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat
    33181 cggcgttctg gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca
    33241 cgttgctgca agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga
    33301 aaaagaatac caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc
    33361 agagaaaatg gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca
    33421 gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga
    33481 agtgactggc ttcatccgct tcgaagtggg tgaaggcatc gagaaagttg agactgactt
    33541 tgcagcagaa gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag
    33601 ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa
    33661 aagcgacgta caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga
    33721 aacatggcta ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa
    33781 gctctgcagg gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag
    33841 gaaatcaaag aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac
    33901 ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg
    33961 gggatgctgg cgaccgtaat gaacggcctg gcaatgcgtg atgcactgca ccgcgcctat
    34021 gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg
    34081 gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt
    34141 aacccgttct ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat
    34201 gtggtgctga aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca
    34261 accgcaacca tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc
    34321 atggacctgg cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat
    34381 atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc
    34441 acggaataat tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc
    34501 gtaagtctgg ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca
    34561 accggagcgt acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat
    34621 aaaatagcct aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc
    34681 acacttccac tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac
    34741 gtgattagcg atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc
    34801 aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt
    34861 gtcgtggaat attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa
    34921 gattcccgta ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa
    34981 gcgattatgg cgtccgatct tggcctgaac ccgaactctg cgggtagcga catccgtgtt
    35041 ccgctgccgc cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa
    35101 gcagaacaag cgcgtgttgc agtacgtaac gtgcgtcgtg acgcgaacga caaagtgaaa
    35161 gcactgttga aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta
    35221 cagaaactga ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca
    35281 gaactgatgc agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga
    35341 tcggctggcg gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact
    35401 caccattctg ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa
    35461 tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga
    35521 acagtgcctg gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact
    35581 tcttaaaacg atgctacagc aacagggtag ccgcaccgaa gtcttaagtg ggcaacaagc
    35641 cgcttgcgat atggcagcgc ttgaggatgt tgatcaggtg atggcagcca ttgttggcgc
    35701 tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa
    35761 taaagaatca ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc
    35821 gcaattgtta ccggtcgata gcgaacataa cgccattttt cagagtttac cgcaacctat
    35881 ccagcataat ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac
    35941 cgggtctggt ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgccgga
    36001 tcaagcctgc cgtcatccga actggtcgat ggggcgtaaa atttctgtcg attcggctac
    36061 catgatgaac aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag
    36121 ccagatggaa gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga
    36181 cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat
    36241 ggcatggccg aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc
    36301 gttgacattt gccgcaccgg attatgatcg ttatccatgc ctgaaactgg cgatggaggc
    36361 gttcgaacaa ggtcaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc
    36421 tgcttttctt gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact
    36481 ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa
    36541 cgcgcgtgaa gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta
    36601 cagagagtcg cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc
    36661 gtaagtagtt ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat
    36721 aggcttcagt attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg
    36781 tctgctactc aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc
    36841 attatggacg gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat
    36901 aaagccgggg caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag
    36961 gcgttaacgc tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt
    37021 gcgttaatgg aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat
    37081 aacgtgcgtc tgcgtattat tggcgatacc agtcgcttta actcgcgttt gcaagaacgt
    37141 attcgtaaat ctgaagcgct aacagccggg aataccggtc tgacgctgaa tattgcggcg
    37201 aactacggtg gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag
    37261 caaggaaacc tgcaaccaga tcagatagat gaagagatgc taaaccagca tgtctgtatg
    37321 catgaactgg cccctgtaga tttagtaatt aggactgggg gggagcatcg cattagtaac
    37381 tttttgcttt ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat
    37441 ttcgatgaac aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc
    37501 ggcggcaccg agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc
    37561 ctgatatctg cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg
    37621 gtggggttcg ccatcgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag
    37681 cttagcggtt ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg
    37741 ttggcgctga tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt
    37801 gaaatctcac tttgggcttc gctgggttgg tggattgtcg cgctattgct ggtgctgttt
    37861 tacccaggtt ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg
    37921 ctaaccattg ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag
    37981 aatcattaca gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac
    38041 tccggcgcat atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct
    38101 ccgggtaaaa cctggcaagg ctttataggt ggactagcga ccgcagcggt aatctcatgg
    38161 ggttatggca tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt
    38221 gtcgcagcgt tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca
    38281 ggaattaagg acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat
    38341 agcctgacgg ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt
    38401 taacggaagg taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg
    38461 gtgtacttat caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc
    38521 gcgttgagcg tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg
    38581 gcaccgaata tgttatcgcc ctgatcccgc tgggcggtta tgtcaaaatg ctggatgagc
    38641 gcgcagaacc ggtcgttccg gaactccgcc accatgcctt caataataaa tctgttggcc
    38701 aacgagcggc gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct
    38761 actggctggt ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggt gaaatagcag
    38821 ccaattcgat agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg
    38881 gtatcgaaac gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg
    38941 aaagcaccac cattacagta gcgccatttg gcagcgatca acggcgggat gtaaagctcg
    39001 atttacgtca ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc
    39061 gtcctcgtgg gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa
    39121 gcaaggcagg tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc
    39181 agtgggtgac ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa
    39241 tcgaaaggca gggaagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg
    39301 gtaaagcgat tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata
    39361 aagttgtacg ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc
    39421 agctgatgaa gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga
    39481 acaacctcag tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg
    39541 tagtttatta cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt
    39601 ttccgttgcc cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg
    39661 gcggaccggt atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg
    39721 tgctgttaat ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga
    39781 agaacgcata ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag
    39841 cgccaccgta tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca
    39901 gcgtgtcgcc gttggtgcgg ccctcctcag tatgccggtg cgcacaggcg acacggttaa
    39961 tgatgaagat atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt
    40021 tcgcgtcctt cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc
    40081 cagcattact ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga
    40141 ggcttctggt gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa
    40201 aggtctggaa gacttctact acagcgtcgg taaatatagc gccagcgtaa aagctgtcgt
    40261 gaccccgctg ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc
    40321 tgaaatccag caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc
    40381 tcatttccaa ctgcgtgacg aagtgccgtg gtggaacgtg gtaggcgatc gtaaatacca
    40441 gaaacagaaa ctggcgggcg accttgaaac cctgcgcagc tactatctgg atcgcggtta
    40501 tgcccgtttc aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta
    40561 cgtcacggtg aacattaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg
    40621 caaccttgcc gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct
    40681 gtataacggc accaaagtga ccaagatgga agatgacatc aaaaagcttc tcggtcgcta
    40741 tggttatgcc tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt
    40801 taaattacgc gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga
    40861 aggtaacgat acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc
    40921 atggctggga agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt
    40981 tgaaactgtc gataccgata cccaacgtgt tccgggtagc ccggaccagg tagatgtcgt
    41041 ctacaaggta aaagagcgca acaccggtag cttcaacttt ggtattggtt acggtactga
    41101 aagtggcgtg agcttccagg ctggtgtgca gcaggataac tggttaggta caggttatgc
    41161 tgttggtatc aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa
    41221 cccgtacttc actgtagatg gtgtaagcct cggtggtcgt ctcttctata atgacttcca
    41281 ggcagatgac gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt
    41341 gggcttcccg atcaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc
    41401 cctgtccaac atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca
    41461 tccgagcacc tctgatcagg ataatagctt caaaacggac gacttcacgt tcaactatgg
    41521 ttggacctat aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct
    41581 gaccggtaaa gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac
    41641 ggcgacttat gtgccgatcg atgacgatca caaatgggtt gttctggggc gtacccgctg
    41701 gggttatggt gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg
    41761 tggttccagc accgtgcgtg gcttccagtc caatacaatt ggtccgaaag cagtttactt
    41821 cccgcatcag gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg
    41881 cgcgaaagac ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag
    41941 cctcgagttc atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac
    42001 ttccttcttc tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc
    42061 tggatatccg gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca
    42121 atggatgtcc ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga
    42181 tggagacaag gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac
    42241 aaaggaatgt agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc
    42301 acgcaaagaa ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt
    42361 ggttattagc tgcaggtctc ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa
    42421 ttgcaatcgt caacatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta
    42481 acacgctgga aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc
    42541 tgcaggctaa aatgaaaaag ctgcagtcca tgaaagcggg cagcgatcgc actaagctgg
    42601 aaaaagacgt gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg
    42661 atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg
    42721 tgaaatccgt tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt
    42781 acaacagcag cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa
    42841 tgccttcaat tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg
    42901 gcgatatcgt catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt
    42961 tcatggttaa cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca
    43021 tgacccagga cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc
    43081 tgacttacgc gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg
    43141 cacccagtgc ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta
    43201 acgcggtgat tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct
    43261 tcgtaggtaa aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt
    43321 accatgagat ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag
    43381 acggctttgg ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg
    43441 taattattgg cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg
    43501 atgacactat tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg
    43561 tcgtgattgg cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa
    43621 ttggtcgtta ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg
    43681 acaaagtgac ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct
    43741 attcctcagg cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga
    43801 tgaacattga tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag
    43861 actaacgttc catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg
    43921 caggccgtgt tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa
    43981 cactcatact ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt
    44041 actggtggat cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt
    44101 ctctgtcaat gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt
    44161 gctgattctg gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa
    44221 actggaaccg ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc
    44281 ggtcgtgcct ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct
    44341 gacccgtttt aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat
    44401 gtgtgctcgt agccgggagg cctgataagt gattgataaa tccgcctttg tgcatccaac
    44461 cgccattgtg gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat
    44521 cgttggaccc catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa
    44581 tggtcatact aaaattggcc gcgataatga gatttatcag ttcgcctcca tcggcgaagt
    44641 taaccaggat ctgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg
    44701 cattcgcgaa agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt
    44761 gggcagcgac aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa
    44821 ccgctgtatt ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc
    44881 gatcatcggc ggcatgaccg cagtccatca gttctgcatc attggtgcgc acgtgatggt
    44941 tggcggctgc tccggtgtgg cgcaggacgt ccctccttat gtcattgcgc agggtaacca
    45001 cgcaacgccg ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc
    45061 gattaccgct atccgcaatg cgtataagct gatttatcgt agcggtaaaa cgctcgatga
    45121 agtgaaaccg gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga
    45181 tttctttgca cgctcaacgc gcggtctgat tcgttaatga ctgaacagcg tccattaacg
    45241 attgccctgg tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct
    45301 ctgaaagaac gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct
    45361 gaaggctgcg aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg
    45421 ctcggtcgtc tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa
    45481 ctgaagccag atatttttgt tggtattgat gcgcctgact tcaatattac tcttgaaggt
    45541 aacctcaaaa agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg
    45601 cgacagaaac gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct
    45661 ttcgaaaaag cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg
    45721 gctgatgcca tgccgttaga tccagataaa aatgccgccc gtgatgtgct ggggatccct
    45781 cacgatgccc actgcctggc gttgctacca gggagccgtg gtgcagaagt tgaaatgctt
    45841 agtgccgatt tcctgaaaac ggcccagctt ttgcgccaga catatccgga tctcgaaatc
    45901 gtggtgccac tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc
    45961 gcgccagacc tttcagttca tttgctggat gggatgggcc gtgaggcgat ggtcgccagc
    46021 gatgcggcgc tactggcgtc gggtacggca gccctggagt gtatgctggc gaaatgcccg
    46081 atggtggtgg gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa
    46141 actgattatg tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agaattattg
    46201 caggaagagt gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg
    46261 aaaaccagcc acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat
    46321 gccgatgagc aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc
    46381 cgcacacgca gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg
    46441 tcgtcaccgc tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca
    46501 aaaagctgag cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga
    46561 gctggagtct gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga
    46621 ccatgctggc gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga
    46681 ttgatggtaa ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggcgata
    46741 gccgcgtacc ggaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa
    46801 tggcggcgct ggatattgtt ttcccgcaat atggttttgc ccaacacaaa gggtacccaa
    46861 ccgcttttca tctggaaaaa ctggctgaac acggcgcgac cgaacaccat cggcgcagct
    46921 ttgggcctgt caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa
    46981 accggaatct gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact
    47041 cgatgatcga tggcctggcc aaaaccgcac cgttggtaaa aaaggcggcg gcgttgggta
    47101 tgccagcact ggcgatcacc gatttcacca acctttgtgg tctggtgaag ttctacggag
    47161 cgggacatgg cgcagggatt aagcctatcg tcggggcaga ttttaacgtc cagtgcgacc
    47221 tgctgggtga tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga
    47281 atctgacgtt gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca
    47341 tcgatcgcga ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcggacgca
    47401 tgggcgacgt cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg
    47461 cgttttatga agaacacttc ccggatcgct attttcttga gctgatccgc accggcaggc
    47521 cggatgaaga aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg
    47581 tcgtggcgac caacgacgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc
    47641 gcgtcgcgat ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc
    47701 cgcagcaata tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atccccgaag
    47761 cccttgccaa caccgttgag atcgccaaac gctgtaacgt aaccgtgcgt cttggtgaat
    47821 acttcctgcc gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg
    47881 caaaagaggg cctggaagag cgtctggcct ttttattccc tgatgaggaa gaacgtctta
    47941 agcgccgccc ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg
    48001 gcttcccggg ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg
    48061 taccggtagg gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa
    48121 tcaccgacct cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac
    48181 gtgtctccat gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg
    48241 agcacgtagc ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga
    48301 tggcggcgaa agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg
    48361 tcgatcgtat ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg
    48421 aagccgagcc gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg
    48481 acatggcgcg caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg
    48541 ttatcgcacc gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac
    48601 atccggtcac ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact
    48661 tccttggttt gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc
    48721 gggcgaagaa tggcgagccg ccgctggata tcgccgcgat cccgctggat gacaagaaaa
    48781 gcttcgacat gctgcaacgc tcggaaacca ctgcggtatt ccagcttgaa tcgcgcggca
    48841 tgaaggacct gatcaagcgt ctgcaacctg actgcttcga agatatgatc gccctggtgg
    48901 cactgttccg ccccggtccg ttgcaatcag ggatggtgga taactttatc gaccgtaaac
    48961 atggtcgtga agagatctcc tatccggacg tgcagtggca gcatgaaagt ttgaaaccgg
    49021 tactggagcc aacctacggc atcatcctgt atcaggaaca ggtcatgcag attgcccagg
    49081 tgctttctgg ttataccctc ggtggcgcgg atatgctgcg tcgtgcgatg ggtaagaaaa
    49141 agccggaaga gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa
    49201 tcaacgccga actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat
    49261 ttaacaaatc gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag
    49321 cacactatcc tgcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga
    49381 aggtggtggg cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata
    49441 taaactccgg tctttaccat ttccacgtca acgacgacgg cgaaatcgtg tatggtattg
    49501 gcgccatcaa aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag
    49561 gcggctactt ccgcgaactg tttgatcttt gcgcccgtac cgacaccaaa aagttaaacc
    49621 ggcgggtgct ggaaaaactg atcatgtccg gggcgtttga ccgtcttgga ccacaccgcg
    49681 cggcactgat gaactcgctg ggcgatgcgt taaaagcggc agatcaacac gcgaaagcgg
    49741 aagctatcgg tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac
    49801 aatcctacgc cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa
    49861 cgttaggcct gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt
    49921 atgtcggagg cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg
    49981 ctgcggggct cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta
    50041 tctgcacgct ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg
    50101 ataaatacca gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct
    50161 ttgatgactt cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag
    50221 cccgggaaaa atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc
    50281 agcttttaaa ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac
    50341 atctctacta tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg
    50401 tctctccgag cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg
    50461 aactggagtt tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc
    50521 cgattgcaga gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga
    50581 aactggatat taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga
    50641 cgcgtaaaat cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccac
    50701 agcgtcctta taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg
    50761 gcgaccgcgc gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc
    50821 gtccggtgat gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta
    50881 actttggtat gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg
    50941 aacgctttaa gatgccaatc atcaccttta tcgacacccc gggggcttat ccgggcgtgg
    51001 gcgcagaaga gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc
    51061 tcggcgtacc ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga
    51121 ttggcgtggg cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg
    51181 aaggttgtgc gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga
    51241 tgggtatcat cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac
    51301 cgctgggcgg cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc
    51361 tggcggatct ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc
    51421 agcgcctgat gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg
    51481 gtggcccttt tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga
    51541 ggaacacatg aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc
    51601 catcaaagaa ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa
    51661 cagcgttgat ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga
    51721 ctgggatgag tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc
    51781 gctttatgcc ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat
    51841 ggcgctctgg ttttttgaat atgcgatggg gcaggcggaa gatatcgcca ttcgtatgcg
    51901 tcagtacacc gacgaatatc ttgataacat tacaccgccg ttcacgaaag ccttgtttac
    51961 ctacgtcaaa gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata
    52021 tcaaaaaagc ccggttggct gcctgtttta tgattttttc ggcgggaata ccctcaaggc
    52081 tgatgtctct atttcggtca ccgagcttgg ttcgttgctc gaccacaccg ggccacacct
    52141 ggaagcggaa gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa
    52201 cggaacatcg acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct
    52261 gttgatcgac cgcaattgtc ataaatcgct ggcgcatctg ttgatgatga acgatgtagt
    52321 gccagtctgg ctgaaaccga cgcgtaatgc gttggggatt cttggtggga tcccgcgccg
    52381 tgaatttact cgcgacagca tcgaagagaa agtcgctgcc accacgcagg cacaatggcc
    52441 ggtccatgcg gtgattacca actccaccta tgatggcttg ctctacaaca ccgactggat
    52501 caaacagacg ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtacaccca
    52561 ttttcatccg atctaccagg ggaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt
    52621 gatcttcgaa acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcgctgat
    52681 ccacattaaa ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac
    52741 cacctcgccc agttatccca ttgttgcttc ggttgagacg gcggcggcga tgctgcgtgg
    52801 taatccgggc aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga
    52861 ggtccagcgg ttgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca
    52921 ggtggatgaa gccgaatgct ggcccgttgc gccaggcgaa cagtggcacg gctttaacga
    52981 tgcggatgcc gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat
    53041 ggacgaacag ggcaatatga gcgaggaggg gatcccggcg gcgctggtgg caaaattcct
    53101 cgacgaacgt gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag
    53161 tattggtatc gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaaacg
    53221 ctcttacgat ctcaacctgc ggatcaaaaa tatgctaccc gatctctatg ctgaagatcc
    53281 cgatttctac cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg
    53341 taaacacgat cttcccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat
    53401 gacgccacat caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga
    53461 acaactggtc ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct
    53521 gttgatgcca ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat
    53581 gctttgttcc gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca
    53641 ggacgaagac ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc
    53701 ggcttccggg cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa
    53761 caggttcacc atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc
    53821 gatattcttg gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa
    53881 ggggatttgg cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg
    53941 gagcgcccca gtcgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac
    54001 atcgatgcgg cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc
    54061 gatccataca cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa
    54121 ctgtatgagc agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct
    54181 actgtaaaat cgcaccatca tgacactcac gctcaataga caacttctca cctcacgcca
    54241 gattctggtg gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca
    54301 gtggcggacg gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag
    54361 tgccaatgcc gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc
    54421 gctggtggtc gaacgcgtgc aacttgcgca agaaggactg ggcattgagg cccaggcgcg
    54481 gcaggcgcgt tatcaggcat ttgcccgcac cttgttgccc ggtgaagtgc tggtcaccgc
    54541 gcaacatctc gacgatcaat gtgaaacttt tctgctggcg ctaaaacgcg gtagcggccc
    54601 tgccgggctt tcggctatgg cggaagtttc ggagtttgcc ggaacacggc ttatccgccc
    54661 gttgctcgcc cgcacgcggg gagaactgga gcagtgggca ttggcgcatg gtttacgctg
    54721 gattgaagac gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt
    54781 agtgccgtta ttgcagcagc gttggccgca ttttgccgaa gcaacggccc gcagcgccgc
    54841 actttgtgct gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg
    54901 ccaaacgtcg caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgccg
    54961 cgcggcgatt atccgtcgct ggctggcagg gcagaatgca ccaatgcctt cccgcgacgc
    55021 gctggtgcgg atctggcagg aagtggcgct ggcgcgggaa gatgcctccc cctgtttacg
    55081 cttgggcgcg tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac
    55141 cgggcaaagt gaaaccattg tgccgtggca gacgtggcta caaccgctgg aattaccggc
    55201 ggggctggga agcgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga
    55261 agcggtcagc gtgcgtttca aagcggcagg attgctgcat attgtcgggc gcaacggcgg
    55321 acgtaagctg aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac
    55381 gccactgctg ttttatggcg aaacgctgat tgcggcggca ggggtatttg tgacgcaaga
    55441 aggtgtggct gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt
    55501 gaaagccgga taagacgcat caaacgtcgc atccggcgaa agtcaatcag gactcgctca
    55561 ccaccaccgt accgatttcc gggtggctaa agctggtaat tttatccaga cgtagctcac
    55621 gggtttcgcc agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcgc
    55681 tggcttttgc ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct
    55741 ggcaggcgag ctcaagatta tcgtaatcat cacaattgat tggttgatac gtatcattca
    55801 ttgacataat cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc
    55861 tcagaaaggg cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg
    55921 tccggaacgt atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca
    55981 caatattttt ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa
    56041 gcctacgaag ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc
    56101 agcgtcgcaa ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta
    56161 tggcgcttaa agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc
    56221 agtttctcga tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga
    56281 tgctgcgctt gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt
    56341 tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt
    56401 ggattgagct ggggctgccg gatgagcggc ggattaagaa agcctgcacc caggccgcag
    56461 aagtggtgct gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga
    56521 gcaaatgtgt gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga
    56581 aagtaagcgc ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggcgtga
    56641 tctggttatc ggacgataag aataatctgg aagttaactt aaccgcctgg caacaacctt
    56701 catgattgtg atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat
    56761 tcgtgcgcag ggcgcgggcg ggcagcatgt taataagacc tcaacggcca ttcatctgcg
    56821 ttttgacatt cgggcgtcca gcctgccaga gtattacaaa gagcgtctac tcgccgccag
    56881 ccatcatttg atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca
    56941 ggaactgaac cgcgaagccg cgctagcccg gctggtggca gtgattaaag atttaacaac
    57001 agaacaaaaa gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc
    57061 atcgaaagca caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga
    57121 ataaaaagaa ggaatagatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc
    57181 tctttactct gatgggatgt aataaccggg ccgaagtcga tacgctttct ccagcacagg
    57241 ctgccgaact gaaaccgatg ccgcaaagtt ggcgcggcgt gctgccgtgt gctgattgcg
    57301 aaggaatcga aacctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt
    57361 atctcggtgc tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg
    57421 ctgacaagct ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg
    57481 atgcgctgga gatgctcgat cgtgaaggca atccgattga atcgcagttc aactatacgc
    57541 tggaaccggc acaatctagt ttacctatga cgccgatgac cctgcgaggc atgtattttt
    57601 atatggctga tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga
    57661 ataacgcaga gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt
    57721 tactgtcagt agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca
    57781 aagtattagc gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg
    57841 ggcagtaaac ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc t
//
LOCUS       DAMZII010000015        57651 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZII010000015 DAMZII010000000
VERSION     DAMZII010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.015"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            59..616
                     /gene="dsbE"
                     /locus_tag="POA77_000875"
     CDS             59..616
                     /gene="dsbE"
                     /locus_tag="POA77_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDI6364351.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            613..1665
                     /gene="ccmH"
                     /locus_tag="POA77_000876"
     CDS             613..1665
                     /gene="ccmH"
                     /locus_tag="POA77_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDI6364352.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(1700..2347)
                     /gene="narP"
                     /locus_tag="POA77_000877"
     CDS             complement(1700..2347)
                     /gene="narP"
                     /locus_tag="POA77_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDI6364353.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            2667..5258
                     /gene="yejO"
                     /locus_tag="POA77_000878"
                     /pseudo
     CDS             2667..5258
                     /gene="yejO"
                     /locus_tag="POA77_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(5362..5438)
                     /locus_tag="POA77_000879"
     tRNA            complement(5362..5438)
                     /locus_tag="POA77_000879"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5402..5404),aa:Pro,seq:ggg)
     gene            complement(5513..7273)
                     /gene="pbgA"
                     /locus_tag="POA77_000880"
     CDS             complement(5513..7273)
                     /gene="pbgA"
                     /locus_tag="POA77_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDI6364354.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(7293..7520)
                     /locus_tag="POA77_000881"
     CDS             complement(7293..7520)
                     /locus_tag="POA77_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDI6364355.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            7702..8709
                     /gene="yejK"
                     /locus_tag="POA77_000882"
     CDS             7702..8709
                     /gene="yejK"
                     /locus_tag="POA77_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDI6364356.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     LQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(8848..9132)
                     /gene="rplY"
                     /locus_tag="POA77_000883"
     CDS             complement(8848..9132)
                     /gene="rplY"
                     /locus_tag="POA77_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDI6364357.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(9257..11017)
                     /locus_tag="POA77_000884"
     CDS             complement(9257..11017)
                     /locus_tag="POA77_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6364358.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            11166..11861
                     /gene="rsuA"
                     /locus_tag="POA77_000885"
     CDS             11166..11861
                     /gene="rsuA"
                     /locus_tag="POA77_000885"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDI6364359.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11889..13079
                     /gene="bcr"
                     /locus_tag="POA77_000886"
     CDS             11889..13079
                     /gene="bcr"
                     /locus_tag="POA77_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDI6364360.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            13412..13756
                     /locus_tag="POA77_000887"
     CDS             13412..13756
                     /locus_tag="POA77_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364361.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13760..15349)
                     /gene="yejF"
                     /locus_tag="POA77_000888"
     CDS             complement(13760..15349)
                     /gene="yejF"
                     /locus_tag="POA77_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDI6364362.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(15351..16376)
                     /locus_tag="POA77_000889"
     CDS             complement(15351..16376)
                     /locus_tag="POA77_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6364363.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(16376..17470)
                     /locus_tag="POA77_000890"
     CDS             complement(16376..17470)
                     /locus_tag="POA77_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDI6364364.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(17471..19285)
                     /locus_tag="POA77_000891"
     CDS             complement(17471..19285)
                     /locus_tag="POA77_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6364365.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFHLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPTVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(19367..20923)
                     /locus_tag="POA77_000892"
     CDS             complement(19367..20923)
                     /locus_tag="POA77_000892"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI6364366.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(21104..21670)
                     /gene="mepS"
                     /locus_tag="POA77_000893"
     CDS             complement(21104..21670)
                     /gene="mepS"
                     /locus_tag="POA77_000893"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDI6364367.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(22082..22795)
                     /gene="lpxT"
                     /locus_tag="POA77_000894"
     CDS             complement(22082..22795)
                     /gene="lpxT"
                     /locus_tag="POA77_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDI6364368.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(22834..23820)
                     /gene="yeiR"
                     /locus_tag="POA77_000895"
     CDS             complement(22834..23820)
                     /gene="yeiR"
                     /locus_tag="POA77_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDI6364369.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            complement(23938..25404)
                     /locus_tag="POA77_000896"
     CDS             complement(23938..25404)
                     /locus_tag="POA77_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI6364370.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(25627..26199)
                     /gene="yeiP"
                     /locus_tag="POA77_000897"
     CDS             complement(25627..26199)
                     /gene="yeiP"
                     /locus_tag="POA77_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDI6364371.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            26354..26608
                     /locus_tag="POA77_000898"
     CDS             26354..26608
                     /locus_tag="POA77_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI6364372.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(26605..27786)
                     /gene="setB"
                     /locus_tag="POA77_000899"
     CDS             complement(26605..27786)
                     /gene="setB"
                     /locus_tag="POA77_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDI6364373.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            28154..29284
                     /gene="fruB"
                     /locus_tag="POA77_000900"
     CDS             28154..29284
                     /gene="fruB"
                     /locus_tag="POA77_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDI6364374.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29284..30222
                     /gene="fruK"
                     /locus_tag="POA77_000901"
     CDS             29284..30222
                     /gene="fruK"
                     /locus_tag="POA77_000901"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI6364375.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            30239..31930
                     /gene="fruA"
                     /locus_tag="POA77_000902"
     CDS             30239..31930
                     /gene="fruA"
                     /locus_tag="POA77_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDI6364376.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVEKAA"
     gene            32354..33295
                     /locus_tag="POA77_000903"
     CDS             32354..33295
                     /locus_tag="POA77_000903"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDI6364377.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            33283..34221
                     /gene="psuG"
                     /locus_tag="POA77_000904"
     CDS             33283..34221
                     /gene="psuG"
                     /locus_tag="POA77_000904"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI6364378.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            34315..35565
                     /locus_tag="POA77_000905"
     CDS             34315..35565
                     /locus_tag="POA77_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6364379.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLVGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(35633..36331)
                     /gene="yeiL"
                     /locus_tag="POA77_000906"
     CDS             complement(35633..36331)
                     /gene="yeiL"
                     /locus_tag="POA77_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDI6364380.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            36461..37402
                     /gene="rihB"
                     /locus_tag="POA77_000907"
     CDS             36461..37402
                     /gene="rihB"
                     /locus_tag="POA77_000907"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDI6364381.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            37502..38752
                     /locus_tag="POA77_000908"
     CDS             37502..38752
                     /locus_tag="POA77_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6364382.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(38808..39896)
                     /locus_tag="POA77_000909"
     CDS             complement(38808..39896)
                     /locus_tag="POA77_000909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI6364383.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(39900..40757)
                     /gene="nfo"
                     /locus_tag="POA77_000910"
     CDS             complement(39900..40757)
                     /gene="nfo"
                     /locus_tag="POA77_000910"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI6364384.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(40831..41880)
                     /locus_tag="POA77_000911"
     CDS             complement(40831..41880)
                     /locus_tag="POA77_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI6364385.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            41979..42860
                     /locus_tag="POA77_000912"
     CDS             41979..42860
                     /locus_tag="POA77_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6364386.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            43065..44534
                     /gene="lysP"
                     /locus_tag="POA77_000913"
     CDS             43065..44534
                     /gene="lysP"
                     /locus_tag="POA77_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDI6364387.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            44827..46818
                     /gene="cirA"
                     /locus_tag="POA77_000914"
     CDS             44827..46818
                     /gene="cirA"
                     /locus_tag="POA77_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDI6364388.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(46850..47686)
                     /gene="yeiG"
                     /locus_tag="POA77_000915"
     CDS             complement(46850..47686)
                     /gene="yeiG"
                     /locus_tag="POA77_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI6364389.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            47944..48612
                     /gene="folE"
                     /locus_tag="POA77_000916"
     CDS             47944..48612
                     /gene="folE"
                     /locus_tag="POA77_000916"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDI6364390.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            48629..49786
                     /locus_tag="POA77_000917"
     CDS             48629..49786
                     /locus_tag="POA77_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDI6364391.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYINPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYHWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            49928..50968
                     /gene="galS"
                     /locus_tag="POA77_000918"
     CDS             49928..50968
                     /gene="galS"
                     /locus_tag="POA77_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDI6364392.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            51248..52246
                     /gene="mglB"
                     /locus_tag="POA77_000919"
     CDS             51248..52246
                     /gene="mglB"
                     /locus_tag="POA77_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDI6364393.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            52307..53827
                     /gene="mglA"
                     /locus_tag="POA77_000920"
     CDS             52307..53827
                     /gene="mglA"
                     /locus_tag="POA77_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDI6364394.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            53843..54853
                     /gene="mglC"
                     /locus_tag="POA77_000921"
     CDS             53843..54853
                     /gene="mglC"
                     /locus_tag="POA77_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDI6364395.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(54924..56159)
                     /gene="preA"
                     /locus_tag="POA77_000922"
     CDS             complement(54924..56159)
                     /gene="preA"
                     /locus_tag="POA77_000922"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDI6364396.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELCDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(56153..57391)
                     /gene="preT"
                     /locus_tag="POA77_000923"
     CDS             complement(56153..57391)
                     /gene="preT"
                     /locus_tag="POA77_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDI6364397.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT        14222 a        14038 c        15080 g        14311 t
ORIGIN      
        1 tgtttgatcc tcgctatcgt aagcgcgtga gtccgcaaaa aactgcgccg gaggccgtat
       61 gaagcgcaaa gtattgttaa ttccgttgat tatcttcctg gcgattgccg cggcgctgct
      121 gtggcagctg gcgcgtaatg ccgaagggga tgatccgacc aatctggaat cggcgctcat
      181 tggcaagcct gtgccgaagt ttcgtctcga atcactggac aatccggggc agttttatca
      241 ggcggatgtg ctgactcagg gcaaaccagt gctgcttaac gtctgggcga cctggtgtcc
      301 gacctgccgt gcggaacatc aatatctgaa tcagctttca gcgcagggca ttcgcgtggt
      361 cggcatgaac tataaagacg atcgccagaa ggcaatcagc tggctgaaag agctgggcaa
      421 tccttacgcg ctaagcctgt ttgatggcga cggcatgtta gggctggatc tcggtgtcta
      481 tggcgcgcca gaaaccttcc ttattgacgg caacggtatc attcgctatc gccatgcggg
      541 cgatctcaat cctcgcgtct gggaagaaga gatcaagccg ctgtgggaga aatacagtaa
      601 ggaggccgca caatgaggtt tttattgggc gtgctgatgc tgatgatctc cggctcagca
      661 ctggcgacca tcgacgtgtt gcagtttaaa gatgaagcac aggaacaaca gttccgtcag
      721 ctcactgaag aactgcgctg cccgaaatgc cagaacaaca gcattgccga ttccaactcg
      781 atgattgcca ccgacctgcg tcagaaagtg tatgaactga tgcaggaagg taaaagtaaa
      841 aaagagattg tcgattatat ggtggcgcgt tacggcaact tcgtcactta cgatccgccg
      901 ttaacgccgc tgaccgtgct gctgtgggtg cttccggtag tggctattgg cattggcggt
      961 tgggtcatat acgcccgctc gcggcgtcgg gtacgcgtag tgccggaagc gtttcctgaa
     1021 caaagcgtgc aggaaggtaa gcgtgccgga tatattgttt atctgccggg tattgtggtg
     1081 gcgttaattg tggctggcgt cagctactac cagactggca attatcagca ggtgaaaatc
     1141 tggcagcagg ccacggcaca ggctccggcg ttgctggaca gggcgctgga tccgaaagcc
     1201 gatccgctca acgaagaaga gatgtcgcgt cttgcgctgg ggatgcgtac tcaactgcaa
     1261 aaaaatccgg gagatataga aggctggatt atgttgggcc gcgttggcat ggcgctgggt
     1321 aacgctagta tcgccaccga tgcatacgct actgcgtatc gcctcgatcc gaaaaacagc
     1381 gatgccgcac tgggttatgc tgaagcgttg acacgttcat ctgatcccaa cgacaaccgc
     1441 ctcggtggtg aactgctgcg ccagttggtg agaagcgacc acagcaatat ccgtgtgtta
     1501 agcatgtatg cgtttaatgc ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg
     1561 gagatgatgt tgaaactctt acctgccaac gatactcgcc gtgcggtgat tgaacgtagt
     1621 atcgcgcagg cgatgcaaca tttgtcgccg caggagagta aataaaaaga ggcccgatgg
     1681 tgatgccatc gggctatttt tattgtgccc cgcgttgttg caggaacaga atggtggctg
     1741 ccacgcgtga gcggacattg agtttgcgca gcagattgcg aatatgtact tttactgtct
     1801 gctctgaaat attcaacacc gaggcaatct gtttatttga cagcccctgt gccagctcgt
     1861 gcagaacatc cagctcgcgc tccgtcagca cgctgaaggg atcttcttcc gcgccaaaca
     1921 tttcacgttc acgtaagtac tgattgacgc gttcgctaaa gactttgctg cctttcgctc
     1981 cagcacgaat tgcttccagc aatacttccg ggtcgctgtc tttcaacaga taaccgtctg
     2041 cgcctgcgtc tatcagcgca aagacatcgc tggaggcatc tgatacggtc aggataataa
     2101 tttgcgcggt aacgccatcc ctgcgcaagg cattgagggt atccaggcca ctcatacctt
     2161 tcatattgag atccagcaag atcacgtcga tatccagtct attcgccaga tcgatcgcgc
     2221 tcgcgccgtc gcccgcttcg gcgaccactt caaagccagg atcaagctcc agtaactgac
     2281 gaacaccgcg tcgcataagt ggatgatcat ccacaatcat cacctgaaaa ggcgttgctt
     2341 caggcatagt agtctcctga ggttttatta gaatcattat gtttgagcgt agcgttggta
     2401 acgctaccag gcgcgtgaat gttaccccat tttatgtatg gacatgtagt gaaaactaca
     2461 accttgtcgt tcgctttggc atcattttca ttataaagac ggaattttgc atcagcttct
     2521 catcaagaaa aacaactaaa aaatcaatca caatacttgt gcatttcttt aaacagtttt
     2581 taaaaactga ctccgggtat ggagctatgg gtattttccg tacccaatgc ttttaacagc
     2641 aattaatttc ataggatgaa agctcaattc atcaatctgg ttctgtttct ctttgtcgtt
     2701 ccgcaatatc tgttctggtg gctacagcgt tatattcacc catagcattg gcatcaacag
     2761 ttgagtatgg tgagactgtt gatggtgttg tcctggaaaa agatatccag ctggtttatg
     2821 ggaccgccaa taatacgaaa atcaatcctg gcggagaaca gcatataaaa gaatttggtg
     2881 taagtagtaa tactgaaatt aacggagggt atcagtacat tgaaatgaat ggtaccgcag
     2941 aatactcagt attaaatgac ggttatcaaa ttgttcaaat gggtggcgcg gcaaaccaga
     3001 ctaccctcaa taatggtgtg ctacaggttt atggcgcagc gaatgatccc acgattaaag
     3061 gcgggcgctt aatctttgaa aaagatggga ttaccgtcct tgccgctatc gaaaagggag
     3121 gattactgga ggttaaagag gggggattag cgattgcggt agatcagaaa gcaggcggtg
     3181 ctattaaagc aagcacgcgg atcatggagg tattcggaac aaaccgtctc ggtcagttcg
     3241 aaatcaagaa tggtattgct aacaatatgc tgttggaaaa cggcggaagt ttgcgagttg
     3301 aagaaaatga cttcgcttat aatactactg tagatagtgg cggcttactg gaggttatgg
     3361 atggcgggac tgcaactggc gttgataaaa aagcaggcgg aaaattaatt gtctcaacga
     3421 atgcgctgga agtgagtggt acaaacagta aaggccaatt tagtataaaa gatggtgtgt
     3481 caaaaaatta tgaactggat gatggttccg ggctcattgt tatggaggac acgcaggcca
     3541 ttgatactat ccttgatgag catgccacta tgcaatcgct gggaaaggat actggtacga
     3601 aagtgcaggc aaatgcggta tatgatctcg gtcgatcaga tcagaatgga agtatcacgt
     3661 attcctcaaa agccatctct gaaaatatgg ttatcaacaa tggccgcgct aacgtctggg
     3721 ctggcacaat ggttaacgtt tcagtcagag ggaatgatgg cattcttgag gtcatgaagc
     3781 cgcaaataaa ttatgcaccc gcaatgttgg tgggtaaggt agtggtttct gagggcgctt
     3841 cttttagaac gcatggtgcc gtggatacca gcaaagcgga cgtttcgctc gaaaatagtg
     3901 tatggaccat cattgccgat atcactacga cgaaccaaaa caccctcctc aacttagcca
     3961 accttgcgat gtctgacgca aatgtgatta tgatggatga gccagtgact cgttcatcag
     4021 tgacggcaag tgcggaaaat ttcattacgt tgaccaccaa taccctgtcg ggaaacggca
     4081 atttttatat gcgtaccgat atggctaatc atcagagcga tcagctcaac gtcaccggtc
     4141 aggcaacagg tgatttcaaa atattcgtga cggacaccgg tgccagcccg gcagcaggag
     4201 atagccttac actggtaaca acgggcggcg gtgatgctgc atttacgttg ggcaatgccg
     4261 gaggcgttgt tgatatcggt acgtatgaat ataccttgct ggataatggc aaccatagct
     4321 ggagtttggc agagaatcgc gcgcaaatta ccccttcaac cactgatgtg ctgaatatgg
     4381 cggcagcaca accgctggta tttgatgcag aactggacac cgtgcgtgag cgtcttggta
     4441 gcgtaaaagg cgttaattac gatacggcga tgtggagttc ggcaattaac acccgcaaca
     4501 acgtgaccac tgatgcgggc gctggctttg agcaaacatt gaccgggctg acgctcggta
     4561 tcgatagccg tttctcccgt gaagaaacca gcacaattcg cggcttgttc tttggttact
     4621 ctcattctga tattggtttt gatcgcggcg gcaaaggcaa tgtcgatagc tataccctgg
     4681 gggcttatgc cggttgggag catcagaacg gtgcctatgt tgatggagtg gtgaaagttg
     4741 accgttttgc caacaccatc cattgcaaga tgagtaatgg ggcaacagcg tttggcgatt
     4801 acaatagtaa cggcgcgggt gctcatgtcg agagcgggtt ccgttgggtt gacggattgt
     4861 ggagtgttag accctatctg gcctttaccg gctttaccac agatggtcag gactacacgt
     4921 tatcaaacgg catgcgcgcg gatgtgggaa atacccggat attacgcgct gaagcgggaa
     4981 cggcggtaag ctatcacatg gacctgcaaa acggtacgac gctggaaccc tgactgaaag
     5041 ccgccgtgcg tcaggaatac gccgattcta accaggtgaa agttaatgac gatggcaaat
     5101 ttaataatga tgtggctgga acccgtggcg tttatcaggc tgggataagg tcatcgttta
     5161 ccccgacgtt aagcggtcat ttgtcagtca gctatggcaa tggcgcaggg gtagaatcgc
     5221 cgtggaatac tcaggcgggt gtggtctgga cgttctgata acaaaaaata aacaggctgt
     5281 gatgtgtcac ggtctgttta tcgaattaat tgcagatata aaaaaaacca accgtaaagg
     5341 ttgatttttt cttgggattt ttggtcggca cgagaggatt tgaacctccg acccccgaca
     5401 ccccatgacg gtgcgctacc aggctgcgct acgtgccgac tcgtggctgc taatactacc
     5461 gttttccaca ccgattgcaa gtaagatatt ttgctaactg atttataatt aatcagttag
     5521 cgataaaacg cttctcgtct gtcagcactt gcaataacaa acttaactgt ggtttttcat
     5581 ctttcactct ttcaccacgt aagttgtaag tgcggtattt accgttattg ttcagcacca
     5641 gcgtcttttt cggggtggta attgccagcg tatcgttatc cgctgcagta acccagtaat
     5701 gacggcgttg agggttgaac aaatcctgac cttgcgaata ttcgctggca ggtgtgctga
     5761 catgtagcag gcgttgcatc agcgtcgtca tcagatcggt atgatcagtc agagcattaa
     5821 tacgctgcgc cggcgtgcct ggccagtgaa tcactaatgg cacctgcaga tgaccgtggg
     5881 accagtcaaa ggtttcttcg tcttcgctca gcggaatacc ccgaccggca gtgataatca
     5941 ccaccgtatt gtccagtttg ccagaatcac gcagtgcatt gagcacgcgg ttgatctggt
     6001 catcgacatt gcctgccgcc cggctatatt tccgtgcaaa tgcctgctga ttgctgtcgt
     6061 caatgttagt gccattgaaa gagacccacg agaaccagcg gttatcttct tgtgcgtagc
     6121 ggcccagcca gttgatccac tgcgtggcgg tctgctcgtc ggattgggtg cgtacgctcg
     6181 gcatcgagaa atctgacaac aatgcctggc gatacagcgg gctggtaaag ccatctgatg
     6241 agaataatcc cagctgatag ccttgctgat taagcgcagt aattaacgcc gcaggggtac
     6301 gggtcgacag aatgccgtcc atatagctcg gcgagatgcc atagaacagg ccaaagatgc
     6361 cgttgtctgt agtgttgccg gagctcatat ggcgcgtgaa cgaaatattt tgctcagcaa
     6421 aacctgccag cgcaggcatc tgcttctcga agcgtgagta gttcaggcca tcgacagtaa
     6481 tcaacagcac attctgcccg gtgcccatat cgcgatagcg cagttcgctt aacggatact
     6541 gaacggaaac ggcgtctgga ttaccttgct caataagacg gcgttgatac tcctgcgcat
     6601 caagcagacc atgcttctca agaaaacgtc gcgccgtcat cgggtacgaa agcggcaggt
     6661 tagcgcgctg catggtgatc gggcgataga agttggcatc ggcccagata tacaccacat
     6721 gcgaggcgat aaaggcgata aataagaatg cggccagcgg gcgcgcgaag cgccgacgac
     6781 gcgtcaggct gcgcagcttt tgccagctcc acgtcgcaaa caccagttca agcaataaaa
     6841 taaccggcac gctgatgaac atcagctgcc agtcgcgcgc catctcattt tcgtctgggt
     6901 tgataaccag ttgccagacg atgggattaa gatggagatg gaaacgagta aagacttcgc
     6961 tatcgatcag taatagcgtc attcccgccg ttgccagaat gacggacaaa aacctcatca
     7021 gcctctggga gccgacgata aaggtcagcg ggaagaggat cagcaagtag gtggcgaaca
     7081 ccaggaagct gaaatggccg ataatgctta cgtaggaata aatgcgacca gcaagcgttg
     7141 tcggccagtc ggcgataaac aggtaacggc tgccaatgac gagcgaaagc agaatattga
     7201 acagtgcaaa ccagtgcccc caactgacca tctgggagac tttttcacgg tagcgctgac
     7261 gatgagttac cataacctgt tatctgtttc ccttagtgcg ctttgtcttc gttgatagag
     7321 gactgtaagg cgcgggcaaa agagttggca attgcctggc gttgggccgg ggcaatgctg
     7381 gtattgataa ggttagtgac catgttgcct aacaccatca gggaaaggtc ggtcggagcc
     7441 ttatgttttt ccagtacgtt gagcagctct gcgagcaatt gttcaacctg ttcatcactg
     7501 tagcgggaaa tttgtggcat aaatcgaaat cagttagttg atgaaagggc aacatattac
     7561 cgtagcaacg gttttttttc tgctttttta tgcgttaaat cattctcgtt acgctgcctt
     7621 ctgtaaaatg accgccatct tttattgcgt cgcttaaccg gcggtggttg aataccgccc
     7681 ggtcttaaag gagagtttat catgagtctg gatatcaacc agattgctct gcaccagctt
     7741 atcaagcgtg atgagcagaa ccttgagctg gtgttgcgcg attcattgct ggaaccgaca
     7801 gaaaccgtcg ttgagatggt ggcagaactg catcgggtct atagcgccaa aaataaagct
     7861 tacggcttgt ttagcgaaga gagtgaactg gcgcagacgc tgcgcttaca gcgtcagggc
     7921 gaagaggact ttctggcatt tagccgcgcg gcaaccgggc gcctgcgtga cgaattagcg
     7981 aaatatcctt ttgctgacgg tggattcgtg ctgttttgcc actaccgtta tctggcggtg
     8041 gagtatctgc tggtggcagt tctgagcaac ctgagcagta tgcgcgttaa cgaaaatctc
     8101 gacatcaatc caacccatta tcttgatatc aatcatgcgg atattgttgc gcgtatcgat
     8161 ttaaccgaat gggaaaccaa tccagagtcc acccgctatc tcactttcct gaaagggcga
     8221 gtagggcgca aagtggcgga tttctttatg gatttcctcg gggccagcga agggttaaac
     8281 gcgaaagcgc aaaaccgtgg gctgttgcaa gcggttgatg atttcactgc cgaagcacaa
     8341 ctggataaag cagagctaca gaacgtgcgc cagcaggttt atagctactg taatgagcag
     8401 ctgcaggcag gcgaagagat cgaactggag tcgctgtcga aagagctggc aggcgtcagc
     8461 gaagtgagtt tcacggagtt tgcggctgaa aaaggctacg aactggaaga gagcttcccg
     8521 gcagaccgta gcacactgcg tcagttgacg aagtttgccg gtagcggcgg tggcttgact
     8581 attaactttg atgcgatgct gttaggtgaa cgcatcttct gggatccggc aaccgatacc
     8641 ctgaccatta aaggcacgcc gccgaatttg cgtgaccaat tgcagcgccg gacgtctggc
     8701 ggcaattaat cggttttgtg ttctgaatgc cagatgcggc gtgaacgcct tatctggcct
     8761 gcaaaaaata gcaataccgt aatcaggcat aatctgcaga tacaaaaaac cccgccggag
     8821 cggggttttt tacaacttat tcagcaatta agcgcgaacg aagtcgatgt gctgcagctt
     8881 cggtttgtac gggtgacgct gtacgtcctg agctttaact ttgatttctt taccgtcaac
     8941 aacgatggtc agaacttcgc tgtagaattc agctttagct tgcatgttca tgactttgtc
     9001 gtgatccagc tcgatagcca gcggcgcttc tttgccaccg tagatgattg ccgggaactt
     9061 gttagcggca cgcaggcggc ggctcgcacc cttaccctgc tctttacgta cttctgcgtt
     9121 gatagtaaac atttctttct ctcttaaatt aatcctgcta caggcgaccc agcaacaggt
     9181 aagtgatctg ctttgcgtat gcaaaagcgg gcgagattct atactcaatc gctttataca
     9241 tcaatcaaaa ctaccattaa ccgcgtaatt cgtgcgcccg acgaaaacga ccttcgtaat
     9301 cgaacacttt ttcacgcact tgccagtact gacctttcat gcgggcaacg acaaaatccg
     9361 ggtgtcgcaa taaggcttgc tgggcgagga tatcggcggc ggtgatccag cgcagaggga
     9421 tgcccggtgt gcgcgtatgc gggcggataa aaagctgctc gaaggcagtt cgctgggcgg
     9481 gcgtttgcag acggaaacgc tcactcacat ccgcaccgtc ttcatcgtaa taggtgattt
     9541 tcaaccattc gcctttctcg tcgtgcccat gctgcaaaga catgccgcta cagcgtaata
     9601 ccagcgcatc tttcagtcgt agcgccgctt ttaacatatc atccggatca accagtaccg
     9661 tgtcacattc gcggcagcgg cgagcggcaa tatcattttc tgcgttacat tgcgggcaat
     9721 ttttaaaacg gaaacggaag tcacactgtt cgcgatgacc gtcgtcatct tcaaaccatc
     9781 cctgacagcg gcgaccaaag tgttcaatca atgtcccgtc ggcggtcgtt ttcccccaaa
     9841 aggtgttggc aaaaccgcag gcagggcaga aaacctgtac cggaacgttg tcacttttgc
     9901 ctttcggtgt accaacttcc ggcgcgtaga gatcgtgagg attacccgca taatcaagaa
     9961 ttaaacaatc agtcttgccc ggagcgagac gcagaccgcg cccgacaatt tgttggtaaa
    10021 gactcactga ttcggtaggg cgcagaatgg cgataagatc gacgtgcggg gcgtcaaatc
    10081 cggtggtcag tactgcgacg ttgaccagat agcgaaaacg ctgggcttta aaatcttcaa
    10141 ttaacacatc gcgctcagcg ccgggggtgt cgccagtaat cagtgcagca tcttcggcag
    10201 gcagtaatcc cacaatctct tttgcgtgtt caacggtggc ggcgaaaatc attacccctt
    10261 tgcgcttttc ggcgaactcc atgatctggc tgatgatatg tggcgtaatg cgttgctgtt
    10321 ttttcagctc acggttgaga tcagcttcgc tgaacagccc gttactctgt gcctgcaagc
    10381 ggctgaaatc gtattgcact actggcatat ccagtcgttc tggcggcgtc agatagccgt
    10441 gtttaatcat ataacgcagc ggcagctcat aaatgcagtc acggaaaagg gctttctcat
    10501 cgccgcgtac catgccgtga taatggaact ggtagatcca gcctttgccc aatcgaaaag
    10561 gcgtggcagt cagccccagc aggcgtaagt ggggattcac tttagtcagg tgagtgagga
    10621 tttgctgata ctggctctct tcatcgtcac caatacggtg gcattcatcg acaatcaaca
    10681 gcgaaaattc accctgaaag gcatcaagat tacgagcgac cgactgcacg ctgccaaata
    10741 ccactttacc gtggctctct ttgcgcttta acccagcggc aaaaatatcg gcttccagcc
    10801 ccagcgcctg atactttgca tggttttgcg ccaccagttc tttgacgtgt gccagcacca
    10861 gaacacgacc acgcgccagc cgtgccagtt cggcaatcac caggctttta cctgcgccgg
    10921 tgggcagcac gataacggca ggggttttat gacgacgaaa atggttgagc gtggcatcca
    10981 cggcttcttg ctgatatggg cggagtgtaa aaatcatggt ctcgcgtagg tagattattc
    11041 tgctaatagt atgccatgga tctttttccc ttgagggatg ttattagacc gctatactgg
    11101 gcagacaaca gttcagattt tcgggtcaat gcccgacctc cgtttttaca ggcaaaatca
    11161 cacacatgcg acttgataaa tttatcgcac agcaactcgg cgttagccgt gctattgccg
    11221 ggcgtgaaat ccgcggcaat cgtgtcaccg tcgatggcga aattgttcgt aatgcagcgt
    11281 tcaaactgct tcctgaacat gatgtcgctt acgatggcaa cccgctggcg cagcaacatg
    11341 gtccacgtta cttcatgctc aataagcctc agggctatgt ttgctccacg gacgaccctg
    11401 atcacccaac ggtgctctat tttctggatg aaccggtagc ctggaaactg cacgccgcag
    11461 gacgtctgga tatcgatacc accggtctgg tgctgatgac tgatgatggt cagtggtcgc
    11521 accgcattac ttctccgcgc catcattgcg agaagaccta tctggtgacg ctggaatcac
    11581 ccgtagctga cgatacggca gagcaatttg ccaaaggcgt acagctgcat aacgaaaaag
    11641 atctcactaa gccagcggtg ctggaagtga ttacccctac gcaggtccgt ctgaccatca
    11701 gcgaagggcg ttatcatcag gtgaaacgca tgttcgccgc tgtgggtaac catgtggttg
    11761 agctgcatcg tgaacgtatt ggcggtatta cgctggatgc tgatttagcc cccggtgaat
    11821 atcgtccgtt aactgaagaa gaaattgcca gcgtcgtcta acttttccat gactttcagg
    11881 agcccgttgt gaccacccga cagcattcgt cgtttgctat tgtttttatc cttggcctgc
    11941 tggccatgtt gatgccgctg tcgattgata tgtatctgcc cgcgctaccg gtaatttcag
    12001 cgcagtttgg cgtacctgcg ggcagtacgc agatgaccct cagtacttat attctgggct
    12061 ttgcgttggg gcagttaatc tacgggccga tggcagacag ctttgggcgt aagccggtgg
    12121 ttcttggcgg tacgctggtg tttgccgccg ccgcggtggc gtgtgcgttg gcacaaacca
    12181 tcgatcagct gattgtgatg cgtttcttcc acgggctggc ggcggctgcg gccagcgtgg
    12241 ttatcaacgc tttgatgcgt gatatctacc cgaaagaaga gttctcgcgg atgatgtcgt
    12301 ttgtcatgct ggtgacgacc attgcaccgc tgatggcacc gatagttggc ggctgggtgc
    12361 tggtgtggct gagctggcat tacatcttct ggatcctcgc cgtggcggcg attctggctt
    12421 cggcaatgat cttctttctg atcaaagaaa ccttaccacc ggagcgtcgc cagccttttc
    12481 acattcgtac cactattggt aactttgcgg cgctgttccg tcataagcgt gtcctgagtt
    12541 atatgcttgc cagtggtttc agctttgccg ggatgttctc gttcttaagc gccggaccgt
    12601 ttgtctacat tgaaattaac cacgtcgcgc cggaaaactt tggttattac tttgcactaa
    12661 acattgtttt cctgttcgtg atgaccattt ttaacagccg cttcgtccgc cgtattggcg
    12721 cgttaaatat gttccgctcg gggttgtgga tacagtttat tatggcggcg tggatggtca
    12781 tcagtgcgct gctggggctg ggattctggt cgctggtggt tggcgttgcg gcgtttgtgg
    12841 gctgcgtgtc gatggtgtca tccaatgcga tggcggtcat tcttgatgag tttccccata
    12901 tggcgggaac ggcatcttcg ctggcaggaa ccttccgttt tggcataggg gcaattgttg
    12961 gcgcattgct ttctcttgcg acctttaact ctgcatggcc gatgatttgg tcaattgcat
    13021 tctgcgcaag cagctccatt ctcttctgtc tgtacgccag tcgaccgaaa aaacggtgat
    13081 ctattgcaca acgaggaagc taaaaggctt cctttgttga tgcatgtcaa ccacaaatct
    13141 atcattcccc cgatatatgt ttattttatg taaaatcaat ttatgtaaaa agtcacatca
    13201 ttgtagttaa aaaggttgag ttagatcgca gaaacgggta catatagccc cgcaaacgtg
    13261 accacgcccg cagatattac ttaaatcaga gccatagagg ccactcaggc gaggcatcaa
    13321 tctttacgat ctgtataaag acggattgtt gatgatgtgt taaaattgat gtaaacaaat
    13381 tgtgaagtaa atgtacttcc ggggaaaata agtgacttca ttacaactct caatcgtcca
    13441 tcgtctaccg cagaactatc gctggtctgc tggtttcgca ggttcgaagg ttgaaccgat
    13501 tccgcaaaat ggaccgtgcg gtgacaacag cctggtggcg cttaaattgc ttagcccgga
    13561 tggtgataat gcatggtcgg tgatgtataa actaagccag gcgttaagcg acatcgaagt
    13621 tccatgttcg gtgctggagt gtgaaggtga gccgtgcctg tttgtaaatc gccaggacga
    13681 gtttgctgca acatgccgat tgaaaaattt tggtgtggca attgccgaac ccttttcaaa
    13741 ctacaatcct ttttaagcgt cagctcaacg ccagtagctg acgcgtatac tcctgctgcg
    13801 gtgtggcaaa cacgcgcgcg cacggtcctt gttccactac ttccccttgt cgcagtacga
    13861 taacctgatg acataacgcg cggacaacgt gcaaatcgtg gctgataaac aaataggcca
    13921 gttgatgctt ttgttgcaat gatttcaata gcgtcaatat ttgcgcctgt accgttttgt
    13981 cgagtgatga tgtcggttca tcaagtatga tcagcgaggg cttaagaatt aatgccctgg
    14041 caatcgcaat acgttgtcgc tgaccaccag agaactccgc cggataacgg tggcgtgttt
    14101 caggatctaa tcccacttca tgcatcacgg ctatcacttg ttgttcgcgt tgtgcggcag
    14161 aaagcgtcgg ctggtgaacc cgtaagcctt cctcaataat ctgcaaaacg ttgagtcgtg
    14221 ggttgagcga ggagtttgga tcctgaaata ccacctgaat gcgatgacga ataggtaaca
    14281 gctggcggcg atttaaattt tgcagtggct gaccgtcaaa gatgatgctg ccctgagaat
    14341 taatcagtcg cagcagcgcc agtcccgtcg tacttttccc ggaaccggac tcgcccacta
    14401 aacccagtgt ttcacccgct cgtagcgtaa aactgatgtt tttcaccacc acattatgat
    14461 ccacaatgcg cttcaaaatc cctttgcgaa tggggaaggc aacctgaagc tgttcaacat
    14521 ccagcaacgt tgaggcaggt tctggcaacg gcactggatc gcctgacggt tcactgttga
    14581 gtagcttttg tgtgtaagga tgagtgggtg atgcaaatag cgtagcggcg taattttgct
    14641 cgacacagcg accgttttgc attaccgcca cgcggtgggc cagttttctg acaatgctga
    14701 ggttatgagt aataaacagc atgcccatat tcagctcgcc ttgcagttcg cgcaacagct
    14761 gtaaaatctg cgcctggaca gagacgtcca gtgcggtggt cggttcatcg gcaattaata
    14821 attccggtcg cgttaacagc gccatcgcaa tcatcacccg ctggcgttcg ccgccggaga
    14881 gctgatgcgg ataatctgtc agccgttttg ccgcctggcg gataccaacg cgatcaaggc
    14941 agttaagaat ttcgccacga gccgcttccc gacgcatccc gcggtggagt gaaagcactt
    15001 cataaagctg tttttccagg gtatgcaatg gatttaacga caccatcggt tcctgaaaaa
    15061 tcatggcgat cttattaccg cgtacaccgc gcaacgtttg atcgctgaca tgaagcagcg
    15121 attcgccatg aaaacgaata tcgccggaga gatattcaac cggcggggaa gggagcaggc
    15181 gtaaaattga cagcgcggta acgcttttgc ctgaacctga ctcacccacc agcgccagcg
    15241 tttcgccagc ctcaatctgt agtgaaacat cattgactac tgtacgtacg gtttgctgat
    15301 ggcgaaaacc caccgacaaa ttttcaatcg ctaacagagt ttgcgtcatg ctacaccgcc
    15361 ttattaggat caaatgcgtc gcggacggct tcaccaataa agatcagcaa agacaataat
    15421 atcgccaccg acaagaatgc ggtgatccca agccacgggg cctgaaggtt atttttccct
    15481 tgtaacagca gttcaccgag tgacggtgaa ccgagcggta gaccgaagcc gaggaaatcg
    15541 agcgaggtca gggtggttat cgaactacat aaaataaacg gtaaaaaggt gagggtcgcg
    15601 accatggcat taggcaacat atgacgcagg atgatactgc gatcgctgac gccaagcgcc
    15661 tgtgccgcac gaatgtagtc gaaattacga gtacgtaaaa actccgcccg caccacgccg
    15721 accagactca tccagccaaa caagacagta attgccagca gccaccagaa gttaggctgt
    15781 acgacgctgg aaagtaaaat aatcaaaaac agcgtcggca tccccgacca tacttcaata
    15841 aagcgttgtc cccagagatc gactttaccg ccgtaatagc cttgtagcgc ccccgccagc
    15901 acgcccatca cgctggaaca gagagtcagc atcaggccaa acagaaccga gatccgcgtg
    15961 ccatagagaa tgcgtgccag cacatcgccg ccgttggcat ccgttcccag ccagttttgc
    16021 cgggagggtg gagaagggaa gggcttatcg gtagcaaagt tgatactggt agcaccaaag
    16081 cgaatcggtg cccacagtac ccagccgtta ttttccagcc gttgtttcag ccacgggtcc
    16141 tgataatcag cctgacttgc cagcgggccg ccaaaatcgc tttcgctgta gtttttcaac
    16201 aacgggaaat accaactgcc gtcataacgc accagcaacg gtttatcgtt agcgataagt
    16261 tcagaacaca aactcaaacc aaacaagacg aggaaaatcc ataacgacca gtatccgcga
    16321 cggttatggc gaaaacgcgc ccaacgggcc tgattgacgg ggctgagtcg cgacattaac
    16381 gtccctcaaa atctatacgc ggatcgacca gcgtatagct gatatcactg acaatattca
    16441 gcagcaggcc aatcagggtg aaaatataca aggtaccaaa cattacagga taatcgcgcg
    16501 agacggtcgc ttcgtagccc agtaagccaa ggccattgag tgaaaacatc acttcaatca
    16561 gcagcgagcc ggtaaaaaac atgctgataa acgtcgccgg aaaaccggca atcaccagca
    16621 gcatggcgtt gcggaacaca tgtttccaga gaatattttt ttcacttacc cctttcgcac
    16681 gcgcggtcac cacgtattgc ttgcgcactt catcaaggaa tgagtttttg gtcagcatgg
    16741 tcagcgccgc aaagccacca atcactgtcg ccagcaccgg cagcgtgata tgccacagat
    16801 aatcggtgat tttctgatac cacggcagcg aatcaaagtt agcggaaacc aggccgcgta
    16861 gagggaacag gtcgaaatag ctgccgcccg cgaagaagac aatcagcagg atggcaaaca
    16921 aaaaggccgg aatggcgtag ccgatgatga taaatgcgct actccagacg tcaaagcggc
    16981 tcccattata aacagctttg cgaatgccta acggaatcga caccagatag ataatcagcg
    17041 tgctccacaa tccgagggtg atggaaaccg gcagactgtc tttaatcagc gtcagcaccg
    17101 aggcgctgcg aaaaaggcta tcaccaaaat caaagcggat gtagtcccag agcattttga
    17161 agtaacgttc gtggatcggc ttatcgaaac cgtagcgatg agtgatctcg gcgatcactt
    17221 ctggatctaa tccgcgtccg ccacggtaat tactgtcgct gatattgccg acgcccgttt
    17281 gcgcatggct ggcgcgaata ccttcaccgc ctgcgccggg taatactccg gcattaccaa
    17341 actcaatggc ggcgatggcc tggtcgaccg gaccgccagg cgcaatttgc acgatgaaaa
    17401 agttgatggt gataatcgcc cataatgttg ggatcaccag caacagacgg cgaatcaggt
    17461 aagcgcccat ctactctccc tgttgtctgg ctgacggcag tttggccgct ttattaacgt
    17521 cataccacca ggtatcgata cccaggctgt aaacagggcg tacagtgggt tgggagaatt
    17581 tatcccacca ggcgagacgg tcttccgcca tgtaccacat tggcagcatg taataattcc
    17641 acgttaatac gcgatccaat gctcgcccca acggcagtaa tttttcttta tttccctgcg
    17701 cggcaataat ttggttgatc agcgagtcga taaccgggct ttgcacgccg ggggcattat
    17761 aagtggaatt gatatattcc gatgaccagg aaatctgtaa atcggaactg ggccacggca
    17821 tcgcccgcca taggcgcggc atcatgtcat agtcgcgact gcgcatgcgg ttagtgattt
    17881 gcgagttatc caccttgcga atgtccatgt tgatacccag ccgttgcagg ctgtgctgga
    17941 acggcaatac ccactgacta ttgctgcttg cgggaagcaa taattcaaag ctgagtggct
    18001 gacccgttgt ggcattaacg cgttgctgac ccttcagcac ccagcccgct tcgttgagaa
    18061 gtttgtcggc ttttaacagg ttgtcacgat cgtagccatc gcctttagat accggcggct
    18121 ggtagatttg tgtgaagact tctgacggca gatctttttt cattggtgcc agaagcacca
    18181 gctccgcggc gtcggggtaa tttctggccg cgtattcggt attctgaaaa taactgttcg
    18241 tgcgactcca ggcattgtaa aacaacgcct tgttcatcca ttcaaagtca aaggcgagag
    18301 tgatagcttc ccgcacccgg cgatcgctga ataccggacg ttggatatta aacgccagcc
    18361 agcgcgtatc ctgggctgat tcgttctttt gctcatcttt gatgatgtat tttttatcga
    18421 aatttttacc ggtataacgc gtggcccagt ttttagcgtc gttttccata cgcaaatcaa
    18481 aggcaccggc tttaaacgct tcaaaggcga cattatcatc gaggtaataa tcgtagcgaa
    18541 tggtgtcgaa attccagcgt ccacggttta ccggtaagtt tgctgcccag taatctttca
    18601 cgcgggaata gacaatattt tgccccattt tccaggacgt aattcggtac ggaccactgg
    18661 caagcggagg cgtggcgagc ggatcgctaa gtttgtgatc cttccagtac ttttctggaa
    18721 ataccggcag cgaaaacaga ctcaacatat cttctttgcc gggtttagct aactcaatgc
    18781 gcacggttaa cggcgcaatg gctttgacgg tggtgccttt gtagaccaga tgaaattgcg
    18841 gcacgccttc ggtcataaat ttttgaaaag taaactctac atcgcgggca gtaatgggag
    18901 aaccatcatg aaaacgggcg cgcggattaa tagcgacctc cacccaggaa taatcgtcag
    18961 catagcgtgc gctttcagca atcagcgggt aataactgcc tggttcgtca tcggaagtcg
    19021 taaatagcgt gtcgtacagc tgctcggtgc gtgcgcccgg gttgccgcgc agtgcatagc
    19081 ggttgaaatt atcgaaggta ccgagggctg acaacgttat ctgcccacct tttggcgcgg
    19141 cggggttcac ataatcaaaa tggttgaaat taaacgcgta ccggggttcg cccagcacgg
    19201 caaaggcata gctttccttg atagcctgcg cctgcgcacc aaaggtgaac agagcgataa
    19261 acagcagcag tatgcgcaca atcatctgca acggatttcc ttttactcat cattagccgc
    19321 aatccggcat gctttcacta tgaataataa gggacgtaag cacaccttac cactgcggcg
    19381 tatacggttt cttcagccag cgaacaaaat cgtccagcgg taacgggcga ctaatccagt
    19441 agccttgcat gaaattaacg ccgcgttcgc ttagccatcg cgcttgttcc ggcgtttcga
    19501 ccccttcagc aaccgtcagc atattgaggc gtttcgccag cgtcagcacc gcgtcaagta
    19561 cgggtgaagt gatcgtttcc gtaccgatgg cgttgataaa tccacggtca attttcagat
    19621 aatcgagcgt aaaacgctca agatagataa gcgcgctgtg cccggtgccg aagtcatcaa
    19681 tagcaatttc tacgcctacc gagtgcagcc aggcgaagag ttgtgtggct tcttgctctt
    19741 tcagcatatc gcgctcggta atttccagca caatctggaa atggtgtgcg ggtagggaag
    19801 tgagcagttt ctggatatct gctttaaagc tttcgctgtg cagatggtcc ggcgcaatgt
    19861 taatgccaaa tttgacgcct accggcagca ctttttctaa ttctgcggca tcgcgggcaa
    19921 ttaactcaaa cagatgctga gtcagcggca caatcatctt ttgcgattcg gcaaagttaa
    19981 tgaaggcatc cgggggaatt tctcccgcga caggatgccg ccagcgtagc agtacttcca
    20041 ggcccgttac tcgcaaagct tgtgtatcca ccaccggttg atacgccacg taaaattgtt
    20101 cgcgcttgat ggcggtcatg atttctctgc cggggcgcat acgtacgctc atcaggtaat
    20161 agcagagcag gccaacgaca gttccactca tgccgcccag taaaaatgcg taccacacat
    20221 cgttccatgt ccagtcatct gcataaagcc gaacggtcag aggaatgccc gcaattttag
    20281 tttcacggac tggcatgtcg gttaattcgt taacgttcat caaacgtgaa gaaaaggtag
    20341 atagcgcagt attgccaata atgagggcga cgccatcgta atcttcctgc cgtgaactat
    20401 aaaagagtga aggcgtcagg ttgagattca gagcggcaaa gacgccgcta tttttcagca
    20461 aagggttgcg ataccagatg acgattgcgg gtttgttcgg caccatcggc gtgccgggta
    20521 agatcgccat atcgacgttt ttattaatgt cgagcgccgg aatcaattca ttgagtggaa
    20581 tgtccatctc accagtcgca gatgaacaga atgttttttt atctttcacc agcacaaacg
    20641 tacggacatt catgctaaac gccgcgcgag cggtcagttc ggggttagcc tgctggcagg
    20701 tatctaaggt cagcggctga agccggtcag tagtggattt caggtcggca aaataggtat
    20761 cgagatactt ttgtacgtcg ataatcagtg tgtcgtattt gacttcgtgc ttatgccagg
    20821 ccactaaaaa ttgaaggcaa ctcaccagta tcgcaatcat cacgcctgca acaatgcagg
    20881 taagcaggag cttacgtcca gaattgggag cgcgtatgaa catgattccg ttaatttcct
    20941 gaaattacga caacatttaa aagataacaa tgaccggact atagcctgga tcaagacgcg
    21001 gaaacatcat tttctctgat gaatgaaaac aaaaaagcac tgcctaagca gtgctttcag
    21061 ttaactcgtc aggatagcca agggattgca tccaaacggt ttattagctg cggctgagaa
    21121 cccggcgtgc ttcgttgtaa cgcttcttcc agtacggttc attcatgctg gaaataataa
    21181 caccactgct ggtggaagca tggacaaact gattgttgcc gatataaata ccgacatggc
    21241 gtcccgttga accggcacgg aacagaacta aatcacccgt acgcaaatta ctgcgggaaa
    21301 cagatttacc catttcctgc tgttcgtaag tcgaacgcgg aagttctaag ccaaattgct
    21361 cacggaatgt acgttgtaca aaaccagaac aatcgatacc ttttttagtg ctgccgccca
    21421 gacgataacg tacgcctttc cagtcagcat actgatccat aattcgcgat tttacgtcga
    21481 cattacgaac caggttttca aattcatcct gagaagcttg cagtgatgat gtttcactac
    21541 ccactgcacg tgtctcagga tgcatattct ttgcggtgtt atttgcacta catgcagaaa
    21601 gcagaaccgc tactgcaatc gcggggatcc cgcgcaagat atatctcaaa atcggttgag
    21661 atttgaccat gttgtttgtt ttcccttgaa gtccttaacg acaaatatcg ttataaaaaa
    21721 atgccaagcg acacgagact aattacctgg gcatgttgaa acaattcctt aaggccaaat
    21781 ctgtggacta acgcacaaaa ttatatgctg tgaggataat ctaccgccga ggcaaaacga
    21841 gttcgagatt acccgattgg tgcggtcttg gcgagcggtt ttacatgatt ttttataaga
    21901 aaaagtgata cagaaagtta ataagcggga ttggtcaatt aaagtacgat tgagtgaaac
    21961 gcatgcaatc cattcattta cttagagaat gatttttgcg ttcgatagat gaaagcacgg
    22021 tgcgcatcat caggattatc ctcactataa aaataaacct gatgatgtta attactgtga
    22081 gttatttgtt ttggaaatgt ttgttttttc ccggtagtga tttgtcaaaa aaggtgatta
    22141 atcgatcact taatggcgtc agcaacaccc agggcaaacc gatcaatatc acggtcatcg
    22201 aaccgacaat gatgtcagta aaccagtgtg cgccaatcat tactctggga aacgcaaaaa
    22261 ccacaaaaat aataagggcg ataagacctg caactttgcc gaaataacgc cacatgaatg
    22321 ccgaaaaaat aagcagcatc atgccgtgat cgccgggaaa gctatctcgt gaggcatctt
    22381 tcgtgggaac agagagcagt tcgctgacgc ggttaatgtc ggtaaaagtc aatgttgggc
    22441 tggcccgttt tacaggaatt aatgcctgac ctagctggtt taataccact gcagttaata
    22501 gcatgaccag accaataatc acgatacgtc gtctgcctgg ggcgttttct ttcagccaga
    22561 aactcagcat caacataccc atcgccagca gtgaacaacc gtcgaaggcg cgattgttgg
    22621 tcaatgcaac cagccacaaa aaggccttac tttcgaccag tttctgatta aagaaataaa
    22681 aaatatccgc atcaatcggc aaccagaatc catgattaac gggaatatac caggaaagaa
    22741 acagcgcgag gccgacaata ttcaacaaca ctatttgcgg caaattttta atcatatttt
    22801 ctctaattat cttactgaaa aacgcaggca accttacgcg gtagtcgcta aacgaagctt
    22861 caacagcgtg ctctgcaaag cattccagtc agcttcgctg ctggaaatca gctcaatacg
    22921 actgtccggt ggcgcaacgt tttgcgtttc aatgtgcagg tcatcgccct gacggttgat
    22981 tcgcaccagc ccttcgggaa tacgcagcac gcctttgacg cgttccaccg gtgcaagtcg
    23041 cgcccattcc agaatgccaa tggtgtcgaa taccgtatca gcgtcgaata tccagccgca
    23101 ggcctgatat ccttgcccgc tgttcagact gcgacgccag cgttgatgct ctggcaggct
    23161 taacgctgct aacccttttt tcacgacatg ctgatgagaa tgcgcggcgc tggcgggcaa
    23221 ctcggctaaa ttgcgacgcg gcaaatccag aagatgaccg tcaactttcc catgctcact
    23281 gtgaattaat tgtcgatcgc caccattttg ctgccaccaa cgctgtagcg cttgctcact
    23341 ttcgggcgtc gcacggtcgg atttattggc gacaatgatg tctgcggcag ccagctggtc
    23401 acggaagttt tcattgctgg cgcttttttc gtccagcagc aggcgtggat cgagaatgca
    23461 caaggtggcg cgcagatcta tccacggttc atagactggt gcggttaaca gatcgaggat
    23521 ctgtttcgga tggcccagcc cggtcggctc tatcaacaag cggtctggtt ttccctgacg
    23581 cagtaaggta ttcaacccta cctgcatggg taaaccatta acgcagcaca tgcagccgcc
    23641 ggggatctct ttcagcaatg cgccgctatc ggcgagcaaa gcaccatcaa ttccgacttc
    23701 cccaaattca ttaaccagta ccgcccattt ttcgttggga tctttatggg ctaacagatg
    23761 aagaatcgac gtggttttcc cgctgccgag aaaaccggtg atgaggttgg tcctggtcac
    23821 aacggctccg caatactctg gtaagttaac tgagcgttaa tcctggctaa aaaaggcaga
    23881 aaagagaagg ggagagcgga cgaagatgag cttcatccgc tttaaccgca gaaatcgtta
    23941 aattttgagg gtgtttaata gcgcctgatg tgcgccgaat tgtacgattt gttgccaggc
    24001 ttgttcgatg gcctgcacaa aatgcgggtt atctggcaga tcatcaccga aaacttcacg
    24061 cagggaaagc agggcggtta cgcgttgttc actgctgctg cccgcaacaa gttcgcgaat
    24121 tttatcgcta agcggatcgc gaacatcaat ggcatttccg gcatcatcaa cgccgctgac
    24181 gtaacgcatc cagccagcaa cgcctaatgc cagcaacgac cagtccgttt cgcaccccag
    24241 atgtatgcga atacctgcca gcatacgttg cggtaatttc tggctaccat ccattgcgat
    24301 ttgccaggtc ttatgtttca gcgccggatt agcaaaacgt gcaattaact tgtccgcata
    24361 ttgtgttaaa tcgacatctt taatttgtag tgtcggcgct tgctcattca gcattaatgt
    24421 tctggcggca tggcgaaatg cgcgatcctg catgcaatca ctgatatggg cgaatcctga
    24481 aaggtaaccc agataagcga gaaaagagtg gctgccatta agcatacgca gtttcatctc
    24541 ttcccatggc aggacatcat tcaccatttg tacacctgcg acttcccagg caggacgccc
    24601 agcgacgaag ttatcttcca ccacccactg gataaacggt tcgcagctaa tcgcgcaggg
    24661 atcatttacc cccagatgct ggctgatttc tgccagtgat tcgtcggttg cagccggaac
    24721 aatgcggtcg accatggttc ccggaaaact gacgtgttct tttatccacc cggcgagttc
    24781 tggcgaacgt ttttctgcca ttcccagcac cgcgtttttc accacatggc cattgtcggg
    24841 aatattgtcg caggagagca cggtaaacgg tgtaaggccg cgctcacggc ggcgtttcag
    24901 tgcttcgacg agaatacccg gtgcggagtg aggttcttca ggggtttgta gatcgtgaat
    24961 aatccgcgga ttactggtgt cgagtgcacc ggtggccggg tcaatacaat agcctttttc
    25021 ggtaatcgtc agggaaacaa ttgccacctg tggctcgcaa aatttctcaa taattgccgc
    25081 taaggaatcc agttttgcat taaggcattc gtgaacggca ccgacaatta tcacctgatt
    25141 gccgtccgca cctttttcca gcacggtata taaatggttc tgtgcgcgga gctggctcat
    25201 cagttgatca ccgctgaaca agctgatttc acagatcccc cagtcgccgc cctgggcatt
    25261 cagcacacga tcggtcagta acgcctgatg agcgcggtga aaggcgccaa agccaaaatg
    25321 aacgatacgt gattgcaact gctggcgatc gtagcgtgga gcgtgtacat gatgcgggag
    25381 cgtaacggag gcaattgtct tcataggagt cacgtcttag taacgataaa ttaacaatga
    25441 cagtgtaaag ttatatgaca gcaattgaaa ttggtctgga ttgttaatgc gatgaagtgt
    25501 gagggcgatc aatcaacgag tgaaaattga gggcgtaagg tgttcaggaa gcgcaaaata
    25561 gaagattggt ataataaatt aaaaaaggga cagcctgagc tatccctttt ctgcatgcgg
    25621 ctgaagttag tcagcacgcc ccatataacg gcgttcttcg atatggatac gaattttttc
    25681 gcctgggctt aagtattctg gtacctgaat caccagacca gtgctcaatg tcgccggttt
    25741 gttacgggcg ctggcagatg cccctttgat gcctggtgca gtttcaacga tttccagatc
    25801 aacggtctgc ggcagctcaa gcgccagcag ttgaccgtcc caggtcagca cctgcatgtc
    25861 cggcatgccg ccctctggca taaacagcaa ctcttcttca atctgatctt tggtgaaggt
    25921 atacggggta tagtcttctt tatccataaa gacatattcg ttgccatcga cataggagaa
    25981 gtcaacgtaa cggcgggtca gcgtcacggt gtcaacgata tcatcacctt tgaaacgctc
    26041 ttctactttc agcccggtac ggacatcaga aaaacgcatt ttgtacagcg tagcggcacc
    26101 gcgggcagtg ggcgactgaa tatcaatatc cttaaccagc agcaatttgc cgttgtaatt
    26161 cagtaccata ccttttttaa tttcgttcgc tcttggcatc gaaaaattcc tgtagtcgaa
    26221 aatgtcaaaa atatcgcgac aaagtactcg cgacgggcct ttcgggcaag tggtattcgc
    26281 acttttgctg gtgcaaaaaa ggtggtactg tgcgcgctca tcaatccggt ggttaacctt
    26341 aagagaacaa cgtatgaaat gccgtccggg ttgcggggcg tgttgcactg ccccttcaat
    26401 ttccagccct attcccggta tgccagatgg caagcccgcc aatacgccct gcattcagct
    26461 tgatgaacag caacgctgta aaattttcac ctcgccactg cggccgaaag tctgtgcagg
    26521 cttacaagcc agcgctgaaa tgtgcggtaa ctcgcgtcag caggccatga cctggcttat
    26581 cgatctggaa atgctgactg cgccttaaac atctttaatc cgcagtaagc aaaacagagt
    26641 ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga tctcggcgac
    26701 gatgcccgcc actgatcctg cgatgatcca gcctacgcgc gaagtgttgg tatagagcgt
    26761 ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc cgatgccgcc
    26821 cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg caggtgaatg
    26881 cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc gcattaagaa
    26941 acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga tttccagccc
    27001 ggcggcggtc cccatcatca caccggccag tttctcggga agatgcagtt cgttgataat
    27061 aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg tacaaatgac
    27121 aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc cggtcgccag
    27181 cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc aaacaataaa
    27241 cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta atgcataagc
    27301 cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag agctgaacat
    27361 caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca tttgcgggtt
    27421 agcggtcgag ccaaagctac taagaaagac gccaacgaat agcaaaacaa agtagttgcg
    27481 attccaggca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga caatcagcga
    27541 tttgcgatcg ccgcgcttat cagagcgccc ggcgagaaac tgacttacca gaatcccaat
    27601 gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta cttcatcagt
    27661 aagaaaaata ctgagtgtcg gggtttgcag agcgcccgca ataccggtga gaaaggcaac
    27721 gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg cgggggagtt
    27781 atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct tgtcagaaaa
    27841 taaacagact gcgaaatcaa aagcgccacg aataatcaaa atgccatttc actgttgctg
    27901 attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca tcagcaatta
    27961 ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt tacggctttc
    28021 cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg atcttgtctt
    28081 taaccctaag ctaggttggc gctttttttc tcatagaagc tgaatcgttt caattcagca
    28141 agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg aaaaggccgg
    28201 agacaaagaa gaggcgattc gccagctcgc tgcggcgctg gtgcaggccg gtaacgtagc
    28261 agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt tcctcggcaa
    28321 tggtattgct attccccacg gcactaccga cacccgcgat caggtgctga aaaccggcgt
    28381 tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag cgtacgtagc
    28441 gatcggtatt gctgccagct cggatgaaca tctgggcctg ctacgccagc tgacccacgt
    28501 actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag aagaacttcg
    28561 cgcattgctg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg aaatgctgac
    28621 gctggatatc gccgccagcg atctgctgac tcttcaggcg ctgaacgctg cgcgtctgaa
    28681 agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac aaccgctgaa
    28741 cctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta gcgcgattgc
    28801 ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca tgctggtgag
    28861 tgtggcgatg aatgacgatc agcccattgc ggttcttaag cgtctcgctg atttgttgct
    28921 cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg cgctgctgac
    28981 cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtggtgc gcaatgaaca
    29041 cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat ttaacagtga
    29101 tattaccgtg acaaaccttg atggtaccgg caaaccggca aacggacgta gtctgatgaa
    29161 agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc agggtgcaga
    29221 tgctgaacag gcgctgaaag caatcggcga cgctatcgct gctggtcttg gggagggcgc
    29281 ataatgagca gacgtgttgc tactatcacc cttaatccgg cttatgacct tgttggtttc
    29341 tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct gcatgcggcg
    29401 ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt caccgttggc
    29461 ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga gctgggcatt
    29521 gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct gacggaaaaa
    29581 gacggcgaag tgaccgactt caacttctcg ggttttgaag tcacccccgc cgactgggaa
    29641 cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg tgtcagcgga
    29701 agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg cctgcgtagt
    29761 cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg tttgaaagcg
    29821 gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg ccgtaaactg
    29881 cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg tattgcgcat
    29941 gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg cgaatggatc
    30001 gccaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc tatggttggt
    30061 ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg tctggcgact
    30121 gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg tccgcagttg
    30181 gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga gaggcataat
    30241 gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata tggcgaagac
    30301 cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc cgaacgacgc
    30361 agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga acggtaaaaa
    30421 tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc tgagtgaagc
    30481 caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg ttgccgccag
    30541 cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac acacctttat
    30601 ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga aagttgaaac
    30661 ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg cagcggatct
    30721 ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta aaccgatgta
    30781 tcgtacctct accggtctgg cgctgaagaa aaccgcgcag gaactggata aagcggttgc
    30841 tgaagcaacg ccgtatgaac cggcgggcaa aactcaaacg gcgaccactg aaggtaagaa
    30901 agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc tgccgatggt
    30961 cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag cgtttaaaga
    31021 gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct ttgcgctgat
    31081 ggtgccggta ctggcaggtt atattgcctt ttccattgcc gatcgtccgg gcctcacgcc
    31141 gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg gtggtattat
    31201 tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaattga aactgccaca
    31261 gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc tggtggtcgg
    31321 tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag ggttgactca
    31381 ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc tcggtggcat
    31441 gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg gtgtgggtct
    31501 gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta tggtgccacc
    31561 gctggcaatg ggtctggcaa caatggtggc gcgtcgcaaa ttcgacaaag cgcagcagga
    31621 aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg caattccgtt
    31681 tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtggtg cgctgactgg
    31741 cgcaatctca atggcgattg gtgcgaaact gatggcacca cacggtggtc tgtttgttct
    31801 gctgatccct ggcgctatta cgccggtact gggttacctg gtagcaatta ttgccggtac
    31861 gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg cagtagagaa
    31921 agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtat cattggatga
    31981 gcgcaaaggg aatgaccagc ttgtgaatgt aattttatga atttaaatgg ttttatcttt
    32041 cagtagaaac ttgaaacgtt catcaaacac taaggttatt tcctcaaggg cagattattt
    32101 tctgcccttt tttattgttc ctgttctgtg catcccgtca caaattcctc gcttttgtaa
    32161 ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg cctctctggc
    32221 ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata tgaacacatg
    32281 tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt aattttttac
    32341 cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggctcgg cgaatattga
    32401 tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag gtaaaataaa
    32461 atttacgcct ggtggagtcg ggcgcaatat tgcacaaaac ctggcgttgc tggggaacaa
    32521 agcctggcta ctgagcgccg taggcagtga cttttatggt caatcgctgc taacgcaaac
    32581 caatcaatct ggcgtttatg tcgataaatg cctgattgtg ccaggagaaa atacgtcaag
    32641 ctatttatca ttactcgata ataccggtga aatgctggtt gctataaatg acatgaatat
    32701 tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc agagggcaaa
    32761 ggtcattgtc gcagactgta atatcagtga agaggcactg gcatggattc tggataatgc
    32821 cgccaacgta cccgtatttg tcgatccggt ttcagcgtgg aaatgtgtca aagtgcgcga
    32881 ccgtctaaat cagatccaca ctctcaagcc aaaccgcctt gaagcggaaa ccctgagtgg
    32941 gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc atcaacatgg
    33001 cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg atatcagcgg
    33061 tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg gagcgggcga
    33121 tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg ccgaatctgt
    33181 tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca ataaccccga
    33241 tttatcgatt gccaacgtta tatcgttagt ggagaacgca gaatgtctga attaaaaatt
    33301 tcccctgaat tattacaaat ttccccggaa gtgcaggacg ctttaaaaaa caaaaaaccg
    33361 gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca aaatgcccag
    33421 accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc cactatcgcc
    33481 attattggcg gcgtgatgaa agtgggttta agcaaagaag aaattgaatt actgggtcgt
    33541 gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt tgccgccgga
    33601 aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc cggaattaaa
    33661 gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac cttcgatatt
    33721 tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc cggggcgaaa
    33781 tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt gccgttaatt
    33841 ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt tgacgtcagc
    33901 attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg gcaaagcggg
    33961 ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat gccggaggaa
    34021 tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg cgttattggt
    34081 aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg tgacagcctg
    34141 aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat tgccaaagaa
    34201 taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcacgt actcatcaag
    34261 cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat cattatggat
    34321 ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct gttgtcagtg
    34381 aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca aatcgctatt
    34441 ggtggcatca tgctctactt cccaccggga aagtgggcag tagaacaggc ggcattaggc
    34501 gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg ttcgctggtt
    34561 gggccgaaaa tggatgtctt gtttgacggt gcgggtttta tcttcgcctt tcgcgtactt
    34621 ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg cgtgatgggg
    34681 ctgctgattc gcatccttgg cagcattttc cagaaagctc ttaacatcag caaaatcgaa
    34741 tcttttgtcg cagtcacaac tattttcctc gggcaaaatg agatcccggc gatcgttaaa
    34801 ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg caatttgtag cgggatggcg
    34861 tccattgctg gttcgatgat gattggttat gccggaatgg gcgtaccaat tgactacctg
    34921 ttagcggcat cgctgatggc gatcccaggt ggtattttgt ttgcacgtat tcttagcccg
    34981 gccaccgagc cttcacaggt cacatttgaa aatctgtcgt tcagcgaaac gccgccaaaa
    35041 agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc cgctggtgta
    35101 gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat tatcggcgga
    35161 attggtggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg ctatgtgctg
    35221 gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaatcttgt gggtagcctg
    35281 attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc cccatacctg
    35341 caaacgggcg gcacgctgga agtgaaaacc attgcgatta tctcctttgc gctttgtggt
    35401 tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat ttcgccaaaa
    35461 cgcgcgccgg aaatcgccca gcttggttta cgggcgctgg cagcagcaac gctttccaac
    35521 ctgatgagtg cgaccattgc cgggttcttt attggtttag cttgagtttc attgccggat
    35581 gtacgcgctt attaagtgtt gcatccggca atcgcgctat tgcaaataat ttttactgca
    35641 tcatcccgga gaatttattc tccgggtcca tctccagcgc cagtcctgac aactgctttc
    35701 tgtttttaat tagataccct ttcttgcttt taattaataa accgtcgtga atgaattgcg
    35761 cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct tgcgtatgct
    35821 tttcgtgata aagatcacct tcctgcgaga gtaatataaa tgctgccagg cgattcacta
    35881 acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta tgacttaagg
    35941 tgacgcagag ttttcgtaaa aatagcgtgt cgtttaataa ccgcgggcgg taatgtttca
    36001 tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt tcatggtctt
    36061 tgtcgattaa ctcaatctcg ccaataaaac agggggcggc aaagaaatcg atcagcgaca
    36121 cgcgaccatt agctagcgtg gcgtaaagcc tggcgcggcc tcgcgtcagg taaaacagcc
    36181 aggacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga aacagccgcg
    36241 tatcggctga aacatccgtt aaaaagcaat ccttaaacgc ggattcactc atcaattgct
    36301 gcttaagatc attattgtgg atttctttca cgatgtttat ccgctatgag atttctcata
    36361 tttagcatac gtacgcggtt ggtaagattc aatcatcggc ccggtcgaat tttctttgct
    36421 gtagaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga gaaaaattat
    36481 tctggattgt gatccgggtc atgatgatgc tattgcaatg atgatggcag cgaaacatcc
    36541 ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg ataaaacatt
    36601 aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt atgcggggat
    36661 gccgcaaccc attatgcgtc aacaaatcgt tgccgataat attcacggtg aaaccggact
    36721 ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agtacccacg cggtgaaata
    36781 catcattgat accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct
    36841 ttcaaatatc gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat
    36901 tgtgctaatg ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat
    36961 ctttgctgac ccggaagcag cccgcgtggt gttcacctct ggcgttccat tagtgatgat
    37021 gggcctcgat ctcaccaacc agaccgtttg caccccggac gtgattgctc ggatggaaag
    37081 ggcaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca
    37141 gttcgaaaac tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct
    37201 gattaaccct gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg
    37261 cccttgctat gggcgcaccg tctgcgacga gctgggcgtt cttggcaagc ccgccaatac
    37321 caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg
    37381 cggctacatc aaaacccatt aagcgttgat aacaagccgg gccgatagcg caagatgcag
    37441 gcccggctat ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataatcac
    37501 tatggatgtc atgagaagtg ttctgggaat ggtggtattg ctggcaattg catttttgct
    37561 gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt
    37621 tgtgattggc ggcattatgc tttggttacc gccagggcgt tgggtcgctg aaaaagtcgc
    37681 ttttggcgtg cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc
    37741 gctggtcggg ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag
    37801 agtattaccg gcaattatct tcgttacagc actggtgagt attctctact acatcggtgt
    37861 gatggggatt ttaattcgca ttctcggcgg tatcttccag aaagcgttaa atatcagcaa
    37921 gatcgagtca ttcgtcgcgg tcactaccat tttcctcggg caaaacgaaa ttccggcaat
    37981 cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga tttgtagtgg
    38041 catggcctcg attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga
    38101 atatttgctg gcggcatcgt taatggcgat cccaggcggg atcttgtttg cccgcctgtt
    38161 aagcccggct acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc
    38221 gccaaaaagc attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc
    38281 cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat
    38341 cggcggcgtt ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta
    38401 cctgttggcc ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg
    38461 gagtttgatt gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc
    38521 atatctgcaa acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt
    38581 gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc
    38641 gccacaccgt gcgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact
    38701 ttctaacctg atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat
    38761 ttatgagccg gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc
    38821 actaatgaaa gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc
    38881 gaaattgccg cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga
    38941 aagctatata ccaagcccgc cataaaacca tcgtcagcac caaaactgtc tactgtcgta
    39001 tgcgctggcg cggtcagcaa aaattgttct ccatccttat cgctgcaaaa tacagattca
    39061 tctttcagat aaacaaaaat gcgtttaaca ccttgttgat gcagcgaatt tacagctctg
    39121 atgcgatcgt tgtcgtcttt aatcggttgc ccccacaaaa tttccagttc attttgtgtt
    39181 ggtttcagag tgtggatccg gctgtaccag ttttttactt tgttcgcttt aaattcggaa
    39241 acggtatcga caaacatcgg aatttcatca gcaatggtaa agacccactc cagggcttca
    39301 ggtgtgagat tacaatcagc cagcaccaca ccagcatggc gaatcaagtc ccgtgaagta
    39361 ttgagcagtt gaggcgttag ctgttgcaga atatgtgtgt cattgattgc caggatagtt
    39421 tcttcctgct tattagcgat agcgagataa gttgcagtgc tgtgtccatg cagacgaatg
    39481 cagtttgaaa cgttcacgcc cgcgcggcgc gtttcttcca gtaatgtctc gccgtaaaaa
    39541 tcgttgccaa tagctgatat taaatgcaca tcccgaccaa gcagagccag gttatgcgcg
    39601 atattacgtc cgacgccgcc tgcggaacag tgaacagaac ctggatgaga ggctgcttgc
    39661 gggtaacgga tatccgccat tccgcgaata tccatattga ttgcccccac aaccacacag
    39721 taatcctgct cggtaaggat atatcctttg cctttaatca aacccttgcg catcaaatcc
    39781 ataatatgag cggcaacacg tgagcggctg atttgcagaa tatcggcgat ttcattctgt
    39841 tgaatcagcg ggttacgccg taaaatttta agaatctgct tttccctgtc gttcatcgat
    39901 caggccaccg ctttttcagt ttgttgcgct ttcagccagg cgatctcttc tgcccagata
    39961 tccgggttga tggtttcaag gatcagcgga atgccgtcga aacggtcgtc ttgcatgatc
    40021 cagcggaacg catcatgacc gatattgcct tcaccgaggc tatgatggcg gtcaacgcgg
    40081 ctgccaaagg tacttttcgc atcgttaagg tgcatcccgc gcagatactt aaagccgaca
    40141 atacgggcaa aatcagcgaa cgttttttcg cattcggctg gagtacgcaa atcatacccg
    40201 gcagcgaaag catggcaggt atcaatgcag acgccgacac gggatttatc ttccacgccg
    40261 tcgataatcg ccgcgagatg ttcgaattta aaccctaagt tactgccctg accggcggtg
    40321 ttttcaatca ccgctgttac accttgagtt ttatccagcg caatgttgat ggattcggca
    40381 atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg gtggaagttg
    40441 agcaaagaaa gccccagttg ttcgcaacgc tgcatttcat ctataaaggc atcgcgcgat
    40501 ttttccagag cttcagtgac cggatgtccg aggttaatca gatagctgtc gtggggaaga
    40561 atttgcgccg atgtgtagtg atatttttca caggccgctt tgaattcatc gatggtttgc
    40621 gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc aaacgcggtt
    40681 gcgtcgattt cggcggcgcg aattgcggca tttgccagac cgccagcagc actaacgtgc
    40741 gctccaatgt atttcatgcg aggactcctg ttaaacccgc tggaggaaaa cggtaatgat
    40801 agcgggttaa caggagcaag atgtagtggt ttatgcgatg acgctttgaa tcacatagtt
    40861 aatcgcacca ccaccaacaa tcagccaggc aaacagtacc agtgccatca gcagaggttt
    40921 cgccccggct tttttcagcg cgctgacgtg agtggtcaga cccagcgccg ccatcgccat
    40981 tgccagcagg aaggtatcca gcgttaccag catgttcacc acgctctgcg gtaacaggtg
    41041 gaacgagtta aagatggcaa ctacgatgaa caagatggca aaccacggaa tagtgatttt
    41101 gcttttctcg ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca gcaggatgag
    41161 gaacggagcc agcatcatca cgcgcagcat tttggaaata actgctgcgt tttccgcatc
    41221 cgggctgatg gcatgacctg ccgccaccac ctgcgccact tcgtgcacag tagaaccaat
    41281 gtagataccg aaagtttccg gactaaacca ttgagacatc agcggatata tcgccgggta
    41341 gaggaaaatc gcgacggtcc cgaagataac aacggttgca acagccacgg ttactttact
    41401 ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac agatactgct
    41461 accggcaccg atcaaccagc tggtgtgctt atccagacca aacactttct gccccaggaa
    41521 gcaagccagc aggaaggtac tggacagcgt caacacgtca atgatgatcc cactgatacc
    41581 gacatcggca atttgcgaga acgtcagacg gaagccataa agaatgatac ccagacgtaa
    41641 taaatattgc ttggcaaaca gcacaccacc gtcacagctt ttccagatgt gcggatagat
    41701 ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg agggcactaa acccggcacc
    41761 cgcaaccgcg ggaatggaac caccccacag ggcgaccccg gtgataactg cactcagggc
    41821 taaccccgga ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga tattcgtcat
    41881 aaccctctcc tttaaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt
    41941 atatatttat aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt
    42001 tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg
    42061 cgttgtcgca atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc
    42121 aactgtttga tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc
    42181 cgcgtgcgtt ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca
    42241 acggcgcgat tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta
    42301 tcgcccgtta tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc
    42361 aggacgtgat gcaagcggtg ctggatttcc gcgttgatat tggttttatt gaaggaccgt
    42421 gccacagcac tgaaattatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg
    42481 cgccgacttc gccgttggcc cgtggtccgg tcaccttaga gcagctggcc gctgcgccgt
    42541 ggatcctgcg tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac
    42601 atttaccgaa gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg
    42661 tgcgtcatgg gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg
    42721 caggcacatt aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta
    42781 tacatcatcg gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc
    42841 ccgcgaatgt accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt
    42901 gacgcagcgc cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc
    42961 ctgggcgatc atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt
    43021 ctgctacaat cgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta
    43081 aaaccacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg
    43141 ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg
    43201 caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga
    43261 tgaccagtct cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg
    43321 gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact
    43381 gggcggtgac tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc
    43441 cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact
    43501 acatctcagt tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga
    43561 cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc
    43621 agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg
    43681 cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta
    43741 ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt
    43801 tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt
    43861 acaccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc
    43921 tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga
    43981 cggcggtgct gtcagcgggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc
    44041 tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc
    44101 gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg
    44161 gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct
    44221 ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg
    44281 acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca
    44341 ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg
    44401 actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg
    44461 gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga
    44521 acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt
    44581 caattccatt tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg
    44641 ttatcgtttg cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc
    44701 cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa
    44761 ggggacgtga agaagatgtg agcgataacc cattttattt tcgtagttac ctcatggaga
    44821 tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc cgctatttct
    44881 tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct
    44941 tccgttgaac aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac
    45001 ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa
    45061 ctgacgaacg aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac
    45121 accctgattc tcgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat
    45181 gatttcgatc tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc
    45241 ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa
    45301 aaaatcggtc agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc
    45361 gatcgcggtg atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg
    45421 ctgggaatga aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca
    45481 acgaccaccg ataccggaga aacgccgcgt attgaaggat tctccagccg cgacggcaat
    45541 gtcgaatttg cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac
    45601 cgtcaggatc gtgattccga ctcgctggac aaaaaccgcc tggaacgcca gaactactcc
    45661 gtcagccata atgggcgttg ggattacggc accagcgaac tgaaatacta cggtgagaaa
    45721 gtcgagaaca aaaaccctgg caacagcagc ccgataactt ccgaaagcaa tacggtcgac
    45781 ggcaaataca cgttgccgct gacggcgatt aatcagtttc tcacggttgg cggtgaatgg
    45841 cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg gaaccagctc caaaacgtct
    45901 gccagccagt acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc gctggcgctg
    45961 acgaccggcg tgcgtatgga cgatcacgaa acctacggtg aacactggag tccgcgtgcc
    46021 tacctggttt ataacgccac cgacaccgta acggtgaaag ggggctgggc gacggcattt
    46081 aaagcacctt ctctgttgca acttagccct gactggacga gcaattcctg ccgtggcgca
    46141 tgtaagattg tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg ggagctgggg
    46201 ctttactaca tgggtgaaga aggctggctg gaaggggttg aatccagcgt taccgttttc
    46261 cgtaacgatg tgaaagatcg tatcagcatc agccgtacgt ctgacgtcaa cgctgcaccg
    46321 ggctaccaaa actttgttgg ttttgagacg ggcgctaacg gacggcgcat accggtattt
    46381 agctactaca acgttaacaa agctcgtatt cagggcgtgg aaaccgaact gaaaattccg
    46441 ttcaacgatg aatggaaact gtcgatcaac tacacctaca acgatggtcg tgatgtcagc
    46501 aacggcgaaa acaaaccgct atccgatctg ccgttccata ctgctaacgg tacgctggac
    46561 tggaaaccgc tggcgctgga agactggtca ttctatgttt ctggtcacta taccgggcag
    46621 aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt ataccatctg gaataccggc
    46681 gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag gcgtgctgaa ccttggcgac
    46741 aaggatctca gtcgtgacga ctacagctat aacgaagacg gacgtcgtta ctttatggca
    46801 gtggattatc gcttctgatg agaagatgcc cggcgaaccg ggcggacttt cacttcagta
    46861 aatactgcgc atggaagcgc aggtgatcct ctataaaaga ggcgatgaag tagtaactgt
    46921 gatcatatcc cggctgaata cgcagcgtca tcggccacgc tttctggcgc gcggcttctg
    46981 ccagtaccgc aggttgcaac tggtcggcaa gaaactgatc attatcgccc tgatcgataa
    47041 gcgtcgggat cgcatcctgc gcgttactgg catacatcag cgcgcaactg tcccattcca
    47101 gccatgcatt tttgtcctca cctaaatagc tgctaaacgc tttgattccc cacgggacgc
    47161 tgcacggatt cacaattggc gcaaaggccg aaacgctggt gtatttaccc ggatttttca
    47221 gcgccataat cagcgcaccg tgaccaccca ttgagtgacc gctaatggcg cagcggtcgc
    47281 tgacattaaa ttgcgactga accagcgccg gtaattcatc gcgcagataa tcatacatcc
    47341 ggtaatgcgt cgcccacggc ggttgcgtgg cattaagata aaagcctgcg ccctggccta
    47401 aatcgtagcc atcgtcgttg gcaacctttt cgccgcgcgg gctggtgtct ggcatcacca
    47461 gtacaatccc cagttctgcc gccacgcgct gggcacccgc cttggtggtg aagttctcgt
    47521 cattgcaggt taatccggaa agccagtaca gcactggtgg cggggtgtga tcacgagggg
    47581 gtgggagaaa gatactgaac gtcatcgggc agtttaaggt actggagtcg tgtcgccagc
    47641 gttgctgcca gccttcaaaa cagcggtgct cttcgagcat ttccatgcgt ggctccttgt
    47701 tgtgttgttt gcaatttctt catcataccg tttattcaat gtgctgtgag taactttcac
    47761 ttccgtattt gcataacgat gttttaacat ctgctgatga aaggcagcgg caattacaat
    47821 aattatcgct gtgaatactg gattatgtgc gccgcctcac gcacaataat caggctgtaa
    47881 atcagcttaa taactttgcc cccacgcagg gcggaggcgt cacacctgca ggagaaatca
    47941 taaatgccat cactcagtaa agaagcggcc ctggttcatg aagcgttagt tgcgcgagga
    48001 ctggaaacac cgctgcgccc gcccgtgcat gaaatggata acgaaacgcg caaaagcctt
    48061 attgctggtc atatgaccga aatcatgcag ctgctgaatc tcgacctggc tgatgacagt
    48121 ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg atgaaatttt ctccggtctg
    48181 gattacgcca acttcccgaa aatcaccctc attgaaaaca aaatgaaggt cgatgaaatg
    48241 gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac accattttgt taccatcgat
    48301 ggcaaagcga cggtggctta tatcccgaaa gattcggtga tcggtctgtc aaaaattaac
    48361 cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg aacgtctgac gcagcaaatt
    48421 cttattgcgc tacaaacgct gctgggcacc aataacgtgg ctgtctcgat cgacgcggtg
    48481 cattactgcg tgaaggcgcg tggcatccgc gatgcaacca gtgccacaac aacgacctct
    48541 cttggtggat tgttcaaatc cagtcagaat acgcgccacg agtttctgcg cgctgtgcgt
    48601 catcacaact gattaaaagg caggaaccat ggagcgcaac gtcacgctcg attttgttcg
    48661 cggcgtcgcc attctgggga tcctgctatt aaacatcagc gcctttgggc taccaaaggc
    48721 ggcttatatc aatcccgcct ggtacggtgc tattacgccg caggatgcat ggacctgggc
    48781 atttcttgat ctcatcggcc aggtgaaatt cctcacgctt tttgcgctgc tgtttggtgc
    48841 tggactgcaa atgttgctgc cccgtggcag acgctggatc cagtcgcggt taacgctgtt
    48901 agtcttattg ggctttattc acggtttatt gttctgggac ggcgatattc tgctggctta
    48961 cgggctggtg ggcttaatct gctggcggct ggtgcgcgat gcgccatcgg taaaaagcct
    49021 gtttaatacc ggcgtcatgc tttatctggt ggggcttggc gttttgctgt tattggggct
    49081 gatttccgac agccagacca gccgcgcctg gacgccggat gcatcggcta ttttgtatga
    49141 aaaatactgg aagcttcacg gcggcgttga tgcgatcagt aatcgtgccg atggtgttgg
    49201 caacagttta ctggcactgg gcgcacagta tggctggcaa ctggctggga tgatgctcat
    49261 tggtgcggcg ctgatgcgca gcggctggct gaaagggcag ttcagcttac gtcactatcg
    49321 tcgtactggt tttgtgctgg tggcgattgg ggtgaccatt aaccttcctg ccatcgccct
    49381 gcaatggcag ctggactggg cgtatcactg gtgtgccttc ttacttcaaa tgccgcggga
    49441 actgagtgcg ccgtttcagg cgattggcta tgcgtcgctg ttttatggct tctggccgca
    49501 attgagccgc tttaagctgg tgcttgcgat cgcctgcgtc ggacggatgg cgctgaccaa
    49561 ctatctattg caaacgctga tttgtaccat gcttttttac cacctcggtt tgtttatgca
    49621 ttttgaccgc ctggagctgc tggcgtttgt tattccggta tggctggcga atatcctctt
    49681 ctctgttatc tggctgcgtt acttccgcca ggggccggtg gaatggctct ggcgtcagtt
    49741 aactttgcgt gctgccggaa cggcaatatc taaaacatca agataacgat ctggatcaca
    49801 ttcgttaaca aaacggctgt aaccgtttcc attgctgtga ctcgattcac gaagtcctgt
    49861 attcagtgct gacaaaatag ccgccagcaa gcagtcattt actgcaatct cataacaggt
    49921 agtgaatatg atcaccattc gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt
    49981 ttcccgggtg ctcaataaca gcacgctggt cagtgccgac acgcgtgaag cagtaatgaa
    50041 agccgtgagt gagctggatt atcggccaaa cgccaatgcc caggcgctgg caactcaggt
    50101 tagcgacacc attggcgtgg tggtgatgga cgtttctgat gcgtttttcg gcgcgctggt
    50161 aaaagcggtg gatctggtcg ctcagcagca tcagaaatac gtgctaatcg gcaatagcta
    50221 tcatgaagcg gaaaaagagc gtcacgccat tgaggtgtta attcgccagc gttgtaatgc
    50281 gttgattgtt cactcaaaag cattgagtga cgatgaactg gcgcaattta tggataacat
    50341 tcccggtatg gtgttaatca accgcgttgt gccggggtac gcccatcgtt gcgtttgcct
    50401 ggataatctc agcggtgccc gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg
    50461 tattggttat ctttcttcca gtcacggcat tgaagatgac gccatgcgta aagcaggctg
    50521 gatgagtgcg ttgaaagagc aggatattat tccgccggaa agctggattg gcactggtac
    50581 gccggacatg ccgggcggtg aggcggcgat ggttgaactg ctggggcgca atctacaact
    50641 taccgctgta tttgcttata acgacaatat ggctgctggc gcactgacag cattaaaaga
    50701 taatggcatt gcgattccgt tacatctctc aatcatcggt ttcgatgata ttcccatcgc
    50761 ccgttacacc gacccgcaat taacgaccgt gcgttatccc attgcttcaa tggcgaaatt
    50821 agccaccgaa ctggccttgc agggggcagc aggcaatatt gatcctcgtg ctagccactg
    50881 ttttatgccg acgttagtgc gtcgccattc tgtcgcaaca cgccagaatg cggcgacgat
    50941 cactaactca acaaatcagg cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat
    51001 cttaacaatg tgatagctat gattgcaccg ttttaacgtt gtaacccgta tgtaacagtg
    51061 aataatcact ttcaccgagg taatagcgtc ataacaacaa ttaaagccgt tttctggagc
    51121 gttaccgggc atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca
    51181 ttaactctac tgccccgccg agcatttatc tcaagcacta ccctgcataa gaaaaaccgg
    51241 agataccatg aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg
    51301 tgccgctgca cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa
    51361 ctttatgtct gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca
    51421 gctgctgatg aatgattctc agaatgacca gtccaagcag aacgatcaga tcgacgtatt
    51481 gctggcgaaa ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac
    51541 ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc
    51601 tcgtaaggcg ctggatagct acgacaaagc ctactacgtt ggcactgatt ccaaagagtc
    51661 cggcattatt cagggcgatt tgattgctaa acactgggcg gcgaatcagg gttgggatct
    51721 gaacaaagac ggtcagattc aattcgtatt gctgaaaggt gaaccgggcc atccggatgc
    51781 agaagcacgt accacttacg tgattaaaga gctgaacgac aaaggcatta aaactgaaca
    51841 gttacagtta gataccgcta tgtgggatac cgctcaggcg aaagataaga tggacgcctg
    51901 gctgtctggc ccgaacgcca acaaaatcga agtggttatc gccaacaacg atgcgatggc
    51961 aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt
    52021 cgatgcgttg ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcacagtact
    52081 gaacgatgct aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg
    52141 caaaggtgcg gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta
    52201 tgttggcgta gataaagaca acctggctga attcagcaag aaataagact gattgggcgc
    52261 agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac
    52321 gactccgtct tccggggaat acttgttgga aatgagcggt atcaacaagt cctttcctgg
    52381 tgttaaggca cttgataacg ttaatttaaa agtccggcca cattctatcc atgcattaat
    52441 gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc ctgtttggta tttatcaaaa
    52501 agactccggc accattttat tccagggtaa agagatcgat ttccattccg ccaaagaggc
    52561 actggaaaat ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt
    52621 gatggataac atgtggctgg ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa
    52681 aatgtaccgc gaaaccaaag cgatttttga tgaactggat attgatatcg atccgcgtgc
    52741 gcgcgtcggc acattatccg tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc
    52801 ctataacgcg aaaattgtga ttatggatga accgacttct tcgttaaccg aaaaagaggt
    52861 caatcatctg ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat
    52921 ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg
    52981 tcagtggatc gccaccgaac cgctggcagg actgacgatg gacaagatca tcgccatgat
    53041 ggttgggcgt tctcttaatc agcgtttccc tgacaaagaa aacaagccgg gcgaagtcat
    53101 cctcgaggtg cgtaacctga cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga
    53161 tctgcataaa ggggagatcc tcggtattgc cgggctggtg ggggcgaaac gtaccgatat
    53221 tgttgagacg ttatttggta ttcgcgagaa atcggctggc accattacgt tgcacggcaa
    53281 aaagatcaat aaccataatg ccaatgaagc cataaaccac ggatttgctc tggtaactga
    53341 ggagcgccgc tcaacgggaa tttatgccta tctggatatt ggttttaact cgttaatttc
    53401 caatattcgc aactacaaaa ataaagttgg tttactggat aactcgcgga tgaaaagcga
    53461 tacccagtgg gtgattgatt cgatgcgggt aaaaacgccg ggtcatcgga cgcaaattgg
    53521 ttcgctctcc ggtggtaatc agcaaaaggt gattattggt cgctggctac taacgcaacc
    53581 agaaatatta atgctcgatg aaccgacgcg cggtattgat gtcggggcga agtttgaaat
    53641 ttatcagtta attgccgaac tggcgaagaa aggcaagggg attattatta tctcctctga
    53701 aatgcctgag ttgttaggga taacagaccg tattctggtc atgagcaatg gtctcgtttc
    53761 cggaattgtc gatacaaaaa caacaacgca aaacgaaatt ctgcgtcttg cgtctttgca
    53821 cctttaagat taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct
    53881 gaaagagggc ggtatttacg tcgttctttt agttttgctg gcgattatta ttttccagga
    53941 cccaacattt ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat
    54001 tattatcgcg ctcggtgtgg cagggttaat tgtcacccag gggaccgatc tttctgctgg
    54061 tcgtcaggta gggctggcgg cagtggtggc tgcgacatta ttgcagtcca tggataacgc
    54121 caacaaagtg ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg
    54181 tgccattggt gcggtgatcg gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac
    54241 gccgttcatt accacgctcg gcacgatgat catcgtctat ggcatcaact cgctctatta
    54301 cgactttgtc ggggcgtcgc caatttctgg ttttgacagt ggcttctcta cctttgctca
    54361 gggctttgtc gcgctgggga gtttccgtct ctcttacatc accttctacg cgttgattgc
    54421 ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc ggtaagaaca tttttgccat
    54481 tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac gtcggcctga acctgctgat
    54541 gatctacgcg ttgtctggcg tgttctatgc ctttggcggg atgttagaag ccggacgtat
    54601 cggctctgcc accaacaacc tcggctttat gtatgagctg gatgctatcg cggcgtgcgt
    54661 ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg attggcgtgg tgaccggggt
    54721 aattattttt accgtcatca actatggcct gacgtatatc ggcgtaaacc catactggca
    54781 gtacatcatc aaaggggcga ttattatctt cgccgtagcg ctggattcac tgaaatacgc
    54841 gcgtaagaag tgatgtgatt gtggatattg ccggatgcgc tgcatccggc aatcggtgcc
    54901 aaatgccgga tgcgtctctg gttttacaac gttaccgggt gttctttctc gcctttctta
    54961 aacttcactt ccccgagctc aatacaaccc accgggcaga cgtgaccaca aagcagacaa
    55021 cccacacatt tctcggtatt acaatgcggt gtgcgggttt tctcgctcca ttccatcgcc
    55081 tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac agccaacaca tttatcaaga
    55141 ttgatacggg gatagacaat ataactgcgg tctaaatctt ccgccgggac aatattgtta
    55201 ttcgccagac ctaccatctc ctgcagcgaa tcaaatccct gatcggcgag ataatgcgac
    55261 aacccgcttg ccatatcttc cactatccga tacccgtact gcatgatgcc ggtggtcacc
    55321 tgtaacgttg ctgcgccgag caataaaaac tcagccgcat cctcccaggt ttcaatgccg
    55381 ccgataccgc tgattgggaa atcgcacagt tctggatggg tgcgcatctg ctgaatgaag
    55441 cgcagggcga tcggttttac cgctttaccg gaatatccgg aaatactcga ttttccgtta
    55501 acgatcggca taccgatttt ctgattaaga tcgatattgg tgatggattt aacggtgtta
    55561 atcgccgcaa tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc gcacatatca
    55621 ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt cactgcccga
    55681 caatattttt ctaccagctc cgggctttgc ccgacatcgc tacccatcgc atgagaagtc
    55741 atttgcggac aggagaagtt acactcgatc atatccgcgc cagcttcttg caccaggcgc
    55801 gccagctcct cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc
    55861 gggtaatctt ccttcagctg acgcagggcg gccagattct cttccaacgg atgttcagca
    55921 atctgctcca tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa
    55981 cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt taaacaccac gccgccccaa
    56041 cctgtgtcgt aggctttggc acacatctca tagcagttgc ctacaggcga agaagagagg
    56101 cagaacgggt tgggaaactt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg
    56161 agcaagctcc ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg
    56221 catagaccac ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc
    56281 gggtctgata attttgtgtt ttgatggtgt tacgctgcgg ctctaactcc gcaaaggcat
    56341 ccagtctggc atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc
    56401 ccggtaaccg tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc
    56461 catcgatgat cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa
    56521 actcatctaa ctcttcacgc gctacgcagg ttaccgcctg acagccgaga actttcagcg
    56581 tgctggctac gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc
    56641 tgatatcgcc ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcac
    56701 tatgctcaaa cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc
    56761 ggttttctgc tttcagctgc tcaagggtga gtgtgttacc gacttcgttg ttgcacttaa
    56821 tggtcacgcc cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc
    56881 ggaattgcgg aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg
    56941 tgacgtcata accctggttt gtcagtgtta cactggcctg caatccggca ggacctgcgc
    57001 caataatcgc gactttgccg agcgttttag taccgggctg ataaatttcc atcccagttt
    57061 gttgttcaaa atcagtaaca aaacgctgta agcgaccgat atcaatggga gtatcaacac
    57121 cggcacgggt acaaccgctt tgacataatt tctccgtcgg gcacactctg gcacaaacgg
    57181 caccgagggc attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga
    57241 ttgagcgaat aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt
    57301 catgacataa taaacagcga gaggcttctt taatcgccag taaaggcgta aaagccggag
    57361 tgagttcatc cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta
    57421 aggcgaacgc taaatatact cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct
    57481 caccccagtc acttacttat gtaagctcct ggggactcac tcacttgtcg ccttcctgca
    57541 actcgaatta tttagagtat aaatattgcg tgctgatatt aaggctatgc gcgtggggaa
    57601 atttatactg tgaaaggatt cacaaatagc tgcggttcag aatgttatat c
//
LOCUS       DAMZII010000016        55030 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZII010000016 DAMZII010000000
VERSION     DAMZII010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.016"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1016)
                     /locus_tag="POA77_000924"
     CDS             complement(<1..1016)
                     /locus_tag="POA77_000924"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6364398.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREG"
     gene            complement(1227..2624)
                     /gene="ccp"
                     /locus_tag="POA77_000925"
     CDS             complement(1227..2624)
                     /gene="ccp"
                     /locus_tag="POA77_000925"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDI6364399.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3029..4678
                     /locus_tag="POA77_000926"
     CDS             3029..4678
                     /locus_tag="POA77_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI6364400.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4729..5331)
                     /locus_tag="POA77_000927"
     CDS             complement(4729..5331)
                     /locus_tag="POA77_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6364401.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5851..6750
                     /locus_tag="POA77_000928"
     CDS             5851..6750
                     /locus_tag="POA77_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6364402.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6799..7812
                     /gene="yhjD"
                     /locus_tag="POA77_000929"
     CDS             6799..7812
                     /gene="yhjD"
                     /locus_tag="POA77_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDI6364403.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            8223..9545
                     /locus_tag="POA77_000930"
     CDS             8223..9545
                     /locus_tag="POA77_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDI6364404.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9779..11839)
                     /locus_tag="POA77_000931"
     CDS             complement(9779..11839)
                     /locus_tag="POA77_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI6364405.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11909..12676)
                     /gene="pdeH"
                     /locus_tag="POA77_000932"
     CDS             complement(11909..12676)
                     /gene="pdeH"
                     /locus_tag="POA77_000932"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDI6364406.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12908..13837
                     /gene="kdgK"
                     /locus_tag="POA77_000933"
     CDS             12908..13837
                     /gene="kdgK"
                     /locus_tag="POA77_000933"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDI6364407.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13933..15429)
                     /locus_tag="POA77_000934"
     CDS             complement(13933..15429)
                     /locus_tag="POA77_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6364408.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15650..16936)
                     /gene="dctA"
                     /locus_tag="POA77_000935"
     CDS             complement(15650..16936)
                     /gene="dctA"
                     /locus_tag="POA77_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDI6364409.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(17119..19107)
                     /gene="hmsP"
                     /locus_tag="POA77_000936"
     CDS             complement(17119..19107)
                     /gene="hmsP"
                     /locus_tag="POA77_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDI6364410.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(19189..22662)
                     /gene="bcsC"
                     /locus_tag="POA77_000937"
     CDS             complement(19189..22662)
                     /gene="bcsC"
                     /locus_tag="POA77_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDI6364411.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVMETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22644..23750)
                     /gene="bcsZ"
                     /locus_tag="POA77_000938"
     CDS             complement(22644..23750)
                     /gene="bcsZ"
                     /locus_tag="POA77_000938"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDI6364412.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGIMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23757..26096)
                     /gene="bcsB"
                     /locus_tag="POA77_000939"
     CDS             complement(23757..26096)
                     /gene="bcsB"
                     /locus_tag="POA77_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDI6364413.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(26107..28725)
                     /gene="bcsA"
                     /locus_tag="POA77_000940"
     CDS             complement(26107..28725)
                     /gene="bcsA"
                     /locus_tag="POA77_000940"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDI6364414.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28722..29474)
                     /gene="bcsQ"
                     /locus_tag="POA77_000941"
     CDS             complement(28722..29474)
                     /gene="bcsQ"
                     /locus_tag="POA77_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDI6364415.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(29486..29674)
                     /locus_tag="POA77_000942"
     CDS             complement(29486..29674)
                     /locus_tag="POA77_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDI6364416.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29947..31518
                     /gene="bcsE"
                     /locus_tag="POA77_000943"
     CDS             29947..31518
                     /gene="bcsE"
                     /locus_tag="POA77_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDI6364417.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            31515..31706
                     /gene="bcsF"
                     /locus_tag="POA77_000944"
     CDS             31515..31706
                     /gene="bcsF"
                     /locus_tag="POA77_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDI6364418.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            31703..33382
                     /gene="bcsG"
                     /locus_tag="POA77_000945"
     CDS             31703..33382
                     /gene="bcsG"
                     /locus_tag="POA77_000945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDI6364419.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(33469..33576)
                     /locus_tag="POA77_000946"
     CDS             complement(33469..33576)
                     /locus_tag="POA77_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6364420.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33952..34059)
                     /locus_tag="POA77_000947"
     CDS             complement(33952..34059)
                     /locus_tag="POA77_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6364421.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(34435..34542)
                     /locus_tag="POA77_000948"
     CDS             complement(34435..34542)
                     /locus_tag="POA77_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6364422.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            35018..36289
                     /locus_tag="POA77_000949"
     CDS             35018..36289
                     /locus_tag="POA77_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6364423.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(36319..37281)
                     /gene="dppF"
                     /locus_tag="POA77_000950"
     CDS             complement(36319..37281)
                     /gene="dppF"
                     /locus_tag="POA77_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDI6364424.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERGKTLAVVGESG
                     CGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQIVFQNPYGSLNPRK
                     KVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPHMFSGGQRQRIAIAR
                     GLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFISHDLSVVEHIADEV
                     MVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRERIKLTGELPSPLNPP
                     PGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR"
     gene            complement(37278..38261)
                     /gene="dppD"
                     /locus_tag="POA77_000951"
     CDS             complement(37278..38261)
                     /gene="dppD"
                     /locus_tag="POA77_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI6364425.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(38272..39174)
                     /gene="dppC"
                     /locus_tag="POA77_000952"
     CDS             complement(38272..39174)
                     /gene="dppC"
                     /locus_tag="POA77_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDI6364426.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(39184..40203)
                     /gene="dppB"
                     /locus_tag="POA77_000953"
     CDS             complement(39184..40203)
                     /gene="dppB"
                     /locus_tag="POA77_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDI6364427.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHSDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(40354..41961)
                     /gene="dppA"
                     /locus_tag="POA77_000954"
     CDS             complement(40354..41961)
                     /gene="dppA"
                     /locus_tag="POA77_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDI6364428.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(42706..42839)
                     /locus_tag="POA77_000955"
     ncRNA           complement(42706..42839)
                     /ncRNA_class="other"
                     /locus_tag="POA77_000955"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(42874..42950)
                     /locus_tag="POA77_000956"
     tRNA            complement(42874..42950)
                     /locus_tag="POA77_000956"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42914..42916),aa:Pro,seq:cgg)
     gene            complement(43042..44733)
                     /gene="eptB"
                     /locus_tag="POA77_000957"
     CDS             complement(43042..44733)
                     /gene="eptB"
                     /locus_tag="POA77_000957"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDI6364429.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(45057..46265)
                     /locus_tag="POA77_000958"
     CDS             complement(45057..46265)
                     /locus_tag="POA77_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364430.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            complement(46494..47192)
                     /locus_tag="POA77_000959"
     CDS             complement(46494..47192)
                     /locus_tag="POA77_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6364431.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            47350..47913
                     /gene="tag"
                     /locus_tag="POA77_000960"
     CDS             47350..47913
                     /gene="tag"
                     /locus_tag="POA77_000960"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI6364432.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            47910..48350
                     /locus_tag="POA77_000961"
     CDS             47910..48350
                     /locus_tag="POA77_000961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6364433.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(48319..50652)
                     /gene="bisC"
                     /locus_tag="POA77_000962"
     CDS             complement(48319..50652)
                     /gene="bisC"
                     /locus_tag="POA77_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDI6364434.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            50805..51464
                     /locus_tag="POA77_000963"
     CDS             50805..51464
                     /locus_tag="POA77_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDI6364435.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            51568..52542
                     /gene="ghrB"
                     /locus_tag="POA77_000964"
     CDS             51568..52542
                     /gene="ghrB"
                     /locus_tag="POA77_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDI6364436.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(52592..53302)
                     /locus_tag="POA77_000965"
     CDS             complement(52592..53302)
                     /locus_tag="POA77_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDI6364437.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            53736..54026
                     /locus_tag="POA77_000966"
     CDS             53736..54026
                     /locus_tag="POA77_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDI6364438.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            54307..54519
                     /gene="cspA"
                     /locus_tag="POA77_000967"
     CDS             54307..54519
                     /gene="cspA"
                     /locus_tag="POA77_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDI6364439.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(54707..54859)
                     /gene="hokA"
                     /locus_tag="POA77_000968"
     CDS             complement(54707..54859)
                     /gene="hokA"
                     /locus_tag="POA77_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDI6364440.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT        13231 a        14892 c        13776 g        13131 t
ORIGIN      
        1 tagccttcac gaccgaggcg caggaattca tagtactgtg caattacctg acccgccggg
       61 cgggagaagt tgatggcaaa agtaccaatt tgaccaccca ggtagtcaac gttgaacacc
      121 agttcctgcg gcagcgcttc ttcgtcacgc cagataaccc agccgcagcc cagcggagcc
      181 agaccgaatt tatggcctga agcactgatc gatttcacac gcggcaggcg gaagtcccag
      241 acgatatccg gggcgacgaa cggtgccagg aagccaccgc tggcagcgtc gatgtgcatg
      301 tcgatgtcga taccagtgtc ggcctggaat ttatccagcg catcgtgcag cggttgtggg
      361 aactcatagt taccggtgta ggtcacgccg aaagtcggca ccacgccgat ggtgttttcg
      421 tcacaggctt caatcatgcg tttcgggtcc ataaacaact gaccggggcg catagggatc
      481 tcacgcagct ccacatccca gtagcgggcg aatttatgcc agcagatttg taccggaccg
      541 cacaccaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt gcgccaacgc
      601 catttcatcg ccatcccgcc aagcatacag gcctcggaag aaccaatggt gttggtgcca
      661 acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat atttacgcaa
      721 cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt tttattgatc
      781 gacaaatcca tcaatttatg gacgttttcg tcgtcccagg tctggcagaa agtggccagg
      841 ttctgacgag cgttgccatc aagatataat tcatcattga taatctggaa tgcgacatca
      901 tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcga tagtagaaat ggcctttgcg
      961 ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg gtccatttcg
     1021 aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa tggaagcaag
     1081 gtattacagg cttaatttaa ataacaaaat cctaagcaga aaaacgactt tttataatat
     1141 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
     1201 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
     1261 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
     1321 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
     1381 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
     1441 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
     1501 ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat aataccacca
     1561 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
     1621 agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
     1681 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggataaact
     1741 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
     1801 gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc ctgcaatgtt
     1861 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
     1921 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
     1981 gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
     2041 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
     2101 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     2161 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     2221 cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac ccattcaatc
     2281 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     2341 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     2401 atataataat aggcgggtaa ttctgccgaa ggtgtgtggc aatagtcgca tcctttttcg
     2461 cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     2521 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     2581 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     2641 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     2701 agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat tacatgaata
     2761 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     2821 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc cgtattttta
     2881 taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg tgatccaccg
     2941 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     3001 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     3061 agacgaactg atgatcgaag tcgatctctg ctatgagctg gacccgtatg aattaaaact
     3121 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     3181 tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     3241 tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc tggatatttt
     3301 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     3361 tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     3421 gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg atcacattcc
     3481 gtggtcttct ctgctggcgc taccgcagtc atatattgtc ccgggcggcc gttttagcga
     3541 aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg gtcgggaaga
     3601 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactatg gtcacatccc
     3661 caacggcaac cgcacctatt atttgagccg atcgcaacca ccggtttttg cgctgatggt
     3721 ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc accttaaaat
     3781 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     3841 tgttgtgcgg atgccggacg gatcgctgct caaccgttat tgggacgatc gcgacacgcc
     3901 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     3961 cgaggtgtac cgcgatttac gcgcgggagc ggcctcaggt tgggattact cttcccgttg
     4021 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     4081 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggcga
     4141 gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     4201 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     4261 ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga accatgaaca
     4321 ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg ggattctggc
     4381 aagcgagtac gaaaccggtg aacagtggga taaacccaat ggctgggcac cgttacaatg
     4441 gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg
     4501 cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga tcgagaaata
     4561 ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     4621 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     4681 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc aggaggagat
     4741 atttaacatc attgccgcct gggtgcgatt tttcacttcc agacggcgat acagggattc
     4801 cagatgcgct tttaccgttc cggtactgat attcagcgct ctgccgatct ccttatttga
     4861 ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg atttgagatc
     4921 tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca tccccatcgc
     4981 cgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca caatggccag
     5041 cacattaaaa tgaataactt cctgtaacca ccgtttattg caatccgtcg ccgtgattaa
     5101 caccttaacc tcaggaaact gcaccacggt tttttgcagc aaccagtagc aaaactcacc
     5161 atcctgatcg ccatcgagca taactaaggc ttcagggtaa ctttccagct tttgccataa
     5221 ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgctgct gtaaactgat
     5281 tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca taactgaatc
     5341 tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc gagaaaaaat
     5401 aagaattcgc catttggcgg tggccattct acagagatga cgcgtagaaa atagttaccg
     5461 atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat ttaaaacaat
     5521 tttgaggatt tccttatatt ggtggttagt acgcatgcaa ttaaaaatga aactccgcga
     5581 ccacaagcaa aaataacaaa cggcaatgag acaaaaataa gcacaaatag ccaacacgtc
     5641 ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaggggg attcaccttt
     5701 ctcagaaagc tattccgccc ttttcctgct gagaaatcgc cacattcgtc atgacaacat
     5761 tgtgaaaccc ggcattagat gttagaaaaa acaataaaca atgcgatatg cgcgttactg
     5821 tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat gcagttgttc
     5881 atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc tttgccaaaa
     5941 ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca gcttctccag
     6001 cgcaccacgc gacgggtcaa actcacgcca gaaggcatga cctattatca acgagcaaaa
     6061 gatgtgttga gtaatctcag cgaactggac ggtctgtttc aacaggatgc caccagtatc
     6121 agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt actgccgcgc
     6181 ctgtcggaat ttctctatct gcatccggga attgagctgg aactgagtag ccatgaccgt
     6241 ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc gttaccggaa
     6301 gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg tcaactgtgc cagtccgcac
     6361 tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca cgcaatagtg
     6421 cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag cgttaatggc
     6481 gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtagcgaaaa ctatctcgcc
     6541 gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg cgaagccctg
     6601 cgtgccgggc ggcttattga agtgttgcca ggctaccgtg ccgagccgct ctccctttcg
     6661 ctggtttacc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat gcagtggctg
     6721 gctggcgtaa tgaaagagca cctggactga ccgactatac tttttaagaa tgaccacgac
     6781 agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac ccaggatctg
     6841 gagcacgagc cgattaagca gttggataat agcgagaaag gcggcaaagt tagccaggcg
     6901 ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccggttat tgcgcacctg
     6961 attcgcgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc ggctatcacc
     7021 tatttctcat ttttatcgat gatcccgatt ttgatggtgt cgtttgccgc agggggcttt
     7081 gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct gcaaaacatc
     7141 agcgatccga cgctagccgc cacgttgaaa aacaccatca acaccgccgt tcagcagcgt
     7201 acgactgtag ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa ctggatgggt
     7261 aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct gggaacgctc gccgcaagat
     7321 caggagaagt tctgggtaaa atatctgcgt gattttattt cgctgattgg tttgttgatt
     7381 gcgctgattg tgacgctttc gatcacctcg gttgccggtt cggcgcagca aatgattatt
     7441 agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt gattggcctg
     7501 gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg gttgccgcgc
     7561 caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat tggttttgaa
     7621 gtgattaaaa tcgtgatgac ctacaccttg ccatcgctga tgaaatcccc ctctggcgca
     7681 gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg tttgacgctg
     7741 ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat gccagggaaa
     7801 acgcagcctt aaaataggcc ggatgcggcg taaacgcctt atccggcata cgattttatt
     7861 cggcctggct ccccgtaggc cggataagat gcgccagcat cgcatccggc tataatgcgc
     7921 acataacctc ttgaaactct tccccagagc ctcttcagcc atctatttgg gagcaaacaa
     7981 tttcattcca actcataacc ccagcatata aatccagttg gtaactttta tttaacctga
     8041 aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag aaaaatcact
     8101 ggcctaaaca ttatcccctt tttgcctggt ttttgaccat ttccgcgatt tgttacacat
     8161 tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa taagaaaaga
     8221 ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgccg atcaactcgc
     8281 gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagttcttc gacttttaca
     8341 tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag ggcgatccaa
     8401 cggcagcaac gctacagtcg ctcgccacct tcgccatcgc cttcgtcgcg cgccccattg
     8461 gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg ctggtcgcct
     8521 cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc tatgccacga
     8581 ttggtatttt cgccccactg ctgctggcgc tggctcgatt tggtcagggt ctgggcttag
     8641 gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg cgcaaacgtg
     8701 cactgtatgg ctccttccca cagctgggcg caccgattgg cttcttcttt gccaacggca
     8761 ctttcttgct gctttcctgg ctactgaccg atgagcagtt tatgagctgg ggctggcgtg
     8821 tgccatttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc gtgtcgctgc
     8881 atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa acaggtgaag atcccgctgg
     8941 gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg tacgttcatc atgctggcaa
     9001 catatacgct gttttacatc atgacggtct actctatgac cttcagtacc gccgccgcgc
     9061 cagttgggct tggcctgcca cgtaacgaag tgttgtggat gctgatgatg gcagttattg
     9121 gttttggcgt gatggtgcca gtcgccggat tactggctga tgcctttggt cgccgtaaaa
     9181 gcatggtaat tatcaccacg ctgatcatcc tgttcgcgct gttcgccttt aacccgctgc
     9241 tcggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta agtctgatgg
     9301 ggctgacctt cgggccgatg ggcgcgctgt taccagagct gttcccaaca gaagtgcgtt
     9361 acaccggagc atcgttctct tacaacgtag cgtcgattct cggggcttcc gttgcgccgt
     9421 atatcgcagc ctggttgcag actaactacg ggttaggtgc ggtggggtta tatctggcgg
     9481 cgatggctgg cttgacgtta atcgccctgc tgctgaccca tgagacgcga catcagtcgt
     9541 tgtaagtatc gtgtgggcag ccaccctcac cccagccctc tccctgaaag ggagagggag
     9601 aaaagcggag cgagatgtta tatcgcgaac ataacagccc tcccccagaa agtgaacgga
     9661 aaagcggtac tcgttgtttc aacacgcaca aaacagtccc ctcgcccctt tggggagagg
     9721 gttagggtga ggggaacacg ctgctcccga actcaccagc aaacacaatc caacctcatc
     9781 acttcttcat ctgcgacaat atcgtccgac actgattagc ctccccttcg gaaggtgaga
     9841 tcagtgccag taacgccgcc gccggtgtta ccagcgttgc cagtgccgca gcaacagcac
     9901 cacgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaac gtcccacgca
     9961 catacagcgg cgaacgcagt gtgataatcc gaatcccttt actctccgga tcaatagtca
    10021 aatccagctg ttccgaagca aaacttgccg tgccggtaac attaatcaac gcgttctcag
    10081 tatcgaaagc aaaaatctgc gggcgcgcca cgccgttggc aatattcaga ttcgccgccg
    10141 cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag ttgccgacat
    10201 tcagcccaac aatctccatc aggttgcggc tcaccagccc gtcattcatc aacagtttca
    10261 ggttgccgtt actgttgcct aaaagcgccg ccaccgagtt accgctaccg cgtagttccg
    10321 cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc atcagttctt
    10381 tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt ttatcgcctt
    10441 ccagatggat attcgccgca atgctgccgc ccgccatgcc aaatttcagc ggttgcaggc
    10501 gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc aggctactgc
    10561 catgctcaat gcgccgccct ttgaagcgaa catcggcatc cataacgtcc catttatcgg
    10621 tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt tcgcccttct
    10681 tctgttcaga ccgtttcgac ttttctgccc ctttcccgga gtcaacgcca attaacggtc
    10741 ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt ggtcgtggct
    10801 ttccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc ccattaaaac
    10861 cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc cgaccatccg
    10921 tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga tcacccagtg
    10981 aatcgccaga aaatttaagc cgtaaatcga caccgcccat cttcattggg tcattcacga
    11041 cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc ggaaacggcg
    11101 tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtacccgtg agcggttcac
    11161 cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt tctttatccg
    11221 ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaac ggatcaacaa
    11281 aaatctccag atccgccttg cttactttgt catcaatagc gatccgccct tgatcgaaaa
    11341 gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct ttgttatcat
    11401 cgttggcgag attaaacgtc cagttattgt tcttttcgga gaggcgaatc aggcgcgcgt
    11461 cgggcttttc gagcttgatc cacggcagcc agaccgtttt ggtcagcagc gccagcgggg
    11521 ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac ttcaggaata tccggtggat
    11581 tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg cgccagccag
    11641 tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg aacggacgat
    11701 tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag tcaaaagttg
    11761 caatcaaaat gatcgccacg acaatcaaca acaagaaagc cactgaaatc gcagcggtta
    11821 ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag tcctaaagat
    11881 agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta ttcagcgttt
    11941 ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc gagttctgaa
    12001 catcacgcca ctcttccggc gtttctacgc cctcaacaat caccccgcga caatagcgat
    12061 tcattagatg taaaagctga gaaaagagtg tgcgtccttc cggcgactga cgcagcatca
    12121 caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt gcagagaaat
    12181 ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac atcgaggcaa
    12241 aggttgaatc tttcggcaga cggatatgct ccaccagttc gaaacgcagc cagggaagac
    12301 gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta gggccatcaa
    12361 tattgaccga tgccagcagg ccgtgctcta taaagaagtc ggctttttgc gccagcaaat
    12421 caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta aaatagcgat
    12481 ccggcggcag gcgttgcgaa gggttcaagg gatgcgcgac caccgttaat agctccacgg
    12541 ccattaaccg cccgcatgtt tgatagatcg gctgccaggt gtaagcacgc tcacactgca
    12601 accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt attcgctgga
    12661 taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc ggactcgaca
    12721 aagattatcg gcgcgtaagg gcagaacttt accgcctggc cggggtaaat ttcctcactc
    12781 cacaacggaa tctttcagga atacgtcctg gctcaaaaaa ataatggaac actgttttaa
    12841 tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac agatcaggtt
    12901 cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc tttccgagaa
    12961 aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg tctatatcgc
    13021 ccgtcaggtc gatcctgcgg cattaaccgt ccattacgta acggcgctgg gaacggacag
    13081 ttttagccag caaatgctgg acgcctggca cggcgagaac gttgatactt ccctgaccca
    13141 acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca ccggtgaacg
    13201 tacgttctac tactggcgta acgaagccgc tgccaaattc tggctggaga gcgagcagtc
    13261 tgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg ggattagcct
    13321 ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg aatgccgcgc
    13381 caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg ccagcaaaga
    13441 agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct tcctgacgct
    13501 ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg cgcgcaccca
    13561 taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc tggtgtccat
    13621 tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa aagtgatcga
    13681 taccaccgca gctggcgact ctttcagcgc cggttatctg gcggtacgtt tgacgggcgg
    13741 cagtgcggaa aacgcggcga aacgcgggca cctgaccgca agtaccgtta ttcagtatcg
    13801 cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg cagcatatgc aaaaggctgg
    13861 ataaagtgtt acccctctat ccagctcatc cataaatacc tgatggttgt gctaactcat
    13921 caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgta cgtcatccgt
    13981 ggcgacagag gtggcggcag cagcagtcga tggggccatg atttgatccc aggccgcctg
    14041 caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta acgccatatc
    14101 attcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc tgttaaggaa
    14161 atcctgccgc agtttctgat actgctccgg cgcgatatcg acgacctgat tttgcaacga
    14221 acgcatccgc tgccccatca gaatatcggt atcagcccgc gcataggcgg caaacagttt
    14281 ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg gcagaccttt
    14341 atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca gcttgtcgtt
    14401 tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc agtcaaatcc
    14461 aagaccgatg tctttgctgt tactggcgct taacgcttgc tgaacatgcc agaacagcgc
    14521 ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac gaatcggctg
    14581 ccacggcgta tcccacatga tagataaccg gtcctgacgc accgcgtcag tcataatact
    14641 caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gctggcgttt cacgtttgcc
    14701 tttcagttcg ccaaacgttt tgtttatttg gtcgacaacc gagcgcgcat ccacgtttcc
    14761 caccaccagt agcgtcattg catccggggt gtaccatttc tgatagaaat ctttaatttt
    14821 ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca aggttgaccc
    14881 tttcagacga tagcgccacc agccctcttt agtatcggca ggccaggttg ccaccatgtc
    14941 ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg gtcaatttgc cagtggcatt
    15001 tgccagataa gagagcgctt ctttcagcaa atcgttacgg ttattgggca aactcagatt
    15061 aaacagcgtg gtgtcataag agacaattac cggcggcatc gggcgtttag ggtcgatccc
    15121 ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg ttagcgcaat
    15181 acgagggatg gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg aaccggtatt
    15241 aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggggtggtca gcacttgcca
    15301 ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga gcgcatctgc
    15361 ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc gaatttttgt
    15421 gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaaactgcc cgttgtcggt
    15481 cagtctaaat gacgttaaaa acacttcgtg ttagaccgca agaacatgaa aatgtcacgg
    15541 aagaagtgaa ataaacctga actcacccag gtggtaagtt caggcacagg ggtcaattat
    15601 gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa gtgatgagat taagaggata
    15661 attcgtgcgt tttgccatcc ggcgcacgat tattcagcac atcgtccagt tttttgtggt
    15721 ccagttcttt cacccactta gcaacgacaa tggtcgctac gccgttaccg accaggttag
    15781 tcagcgcacg agcttctgac ataaagcggt cgataccgag gatcagcgcc agacccgcta
    15841 ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac gataaagcca ctacccgtta
    15901 cccctgccgc ccctttagaa gaaagcagca acacgattaa cagcgtgatt tggtggacga
    15961 tatccatctg actgttagtg gcctgggcga taaacaccgc cgccattgtc aggtatatcg
    16021 atgtgccatc aaggttaaac gagtagcctg tcgggatgac cagccccacc accgatttgc
    16081 ggcagccgag tttctccatc ttgtcgagca tacgcggcag cgccgactcg gaagatgaag
    16141 tccccagtac aatcagcagt tcttcacgga tgtagcggat aaatttgaag atactgaaac
    16201 cagtcgcttt agcgattgaa cccaatacca gcaccacaaa caggatacag gtaatgtaga
    16261 aacagataat cagctgcccc agttgcacca gtgtgccgac gccgtattta ccgatggtaa
    16321 acgccattgc cccgaacgca ccaataggcg ccagacgcat gatcatattg atgatgccga
    16381 agatgacctg cgagaaactt tcgatgacgt taaaaatcag ttggcctttg ctgcccagac
    16441 ggtggagcgc aaaaccaaac agtacggcaa acagcagcac ctgcagaatg ttaccgctgg
    16501 caaatgcgcc aatgacgctc gccgggatga catccataat gaaggcgaca atgccctggt
    16561 ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc ggatcgacgt
    16621 tcattccggc accaggctgc acgacgttaa cgatgataag accaataatc agcgcgatgg
    16681 tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt acgaccgacc gccttcatgc
    16741 tttccatgcc cgcaatgccc gttacgacgg tacaaaagat gacaggagcg atgatcatct
    16801 taatgagctt aacgaagccg tcgccaagcg gtttcatttg ctcgcctatt tcaggataga
    16861 aatggccaag gagaatacca atggctatcg ctgtcaggac ctgaaagtaa aggcttttaa
    16921 acagagaggt tttcataggg tgtcctttag taaaaccaca ggtcttgtaa ggttatgggg
    16981 tacctgcggc cttaaaataa cacccagaca acatcacaga aatgtacctg gatcataatt
    17041 gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt ttaaagtttt
    17101 gtaatcagtt tggggtagct acttttcttc caggtaactc tcttcgaaga tttcaatagg
    17161 gagtgggcga gcaaaaagga agccctgggc aataccaacg cccgcttttg ccagccagtc
    17221 gcgttgtgct tcagtctcca cgccttcggc aatcatttgt aagttcaggc tctgcgccag
    17281 catgataatt gcagcaatca tgctgctatc ttccggcaag ccttcaacaa acattttgtc
    17341 gattttcagt acgtcgattg gcaacgattt catatgctgc agctgacgca gccctgcgta
    17401 gcccatgccg aaatcatcca gcgccacccg aactccggca ttgcgcagcg gacggaggat
    17461 tgccaccgca gcatgagggt cgtcaatacg tcggctttct gtcacttcca gaatcagtgt
    17521 tcccggctga atgcgatagc gggttaacag ttccagcata tccgccacca tattcgggtg
    17581 catcagttgc agcgcagaga ggtttaccga caagggcagc ataatgccgc gctcttgcca
    17641 ggctgcaagc aatcgacagg actcttccag cacccagtga ccgacggtaa ccatgagccc
    17701 acagcactca atgcgatcga ttaagccatc cggcaggtcc caactgccat ccggttgctg
    17761 gatacgcagt aacacttccg cactgaccag tttaccgctg gtcatctcga cctgtggctg
    17821 taaccaaatt gcaaactgat gattttccag tgcattaagg atatcgctct cttccgtcaa
    17881 ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac tgaatctgat tcttgccttt
    17941 atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa agctgttcgg cggtgagatc
    18001 gccgtagaac atcgccacgc caatgctaca gtgcggacgg agttgaatac gttcaatcgg
    18061 caggcgctcg ctcatgatag tgagcacttg ctgacctaag gtgattgcgt gccacggttc
    18121 ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta atctgcgcga ggatcatacg
    18181 tggcgacagt accgatttga gtttttccac cagcgtcagc agcagaattt ctcgttgcgc
    18241 ctctttcagc acgcccgcag tatcacgcag ggtttcacag gtgatgatca tcagcgcggt
    18301 ggtttgttta cgcgcgacaa cctgctccag catctccatc agcaaggctt tgttcggcaa
    18361 atccgacacc gggaagcgca tcgcattttc gttctgttct tcataatggc gctgcagcaa
    18421 ttgctggttg aggttgtaac tgcgcaccaa cataccgatt tcatcgtcct gatgcagacg
    18481 cggtaatgcc agttggtgac caacaagctc ctgggctggg atggcgttaa gttcgcgagc
    18541 aatattgcgt aacggatgca aaatcaggcg gttaatgcac cagctgatgg cgacggtcag
    18601 gataagcgac aaaagtaagt aaatggtcac taacgttgag agggtgctca tcacgaactt
    18661 atacatacgg aaggaatccg cctgtagtac cagataggca attggctgcg ggtttgccgg
    18721 acgttcgagc gagtaaacgc ccagcgagat ttgaaccggt agctcaaaca ggcgagtaac
    18781 cattaccggc accgggcgct ctggaataaa acttttacgc agcgcctgga actggttagg
    18841 cagcactaca tcggcacggc tgaccacgcc tgccggttta atgctggcaa gaatagcttc
    18901 cgcttcggga atatcgcctt tcaaaatagc tgaagataag ggttcgcgga cagagcgagc
    18961 aatgctttcc agttgcgtag ccgtgttata gcgattctgc tggaccagat ggaacagcaa
    19021 aacggtgcaa aaaataaaaa cgaacaccag gacaacggct gccaccattg ccatctgctt
    19081 gattgttaac gagcgactta cgcgcaaatt gtctcccccc ggaaatccca gattgccgcc
    19141 cgagtatacc cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt accagtcggc
    19201 gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc tgccatccgg cggcggaata
    19261 acgtacgtag agcaggaaat ggctgggtgc gtaatctttc gcctgctgga tatcaattgc
    19321 cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt aatgcccgcg ccgtgtagcc
    19381 gaagccctga ctgctgccgc catcgttgga ttgccgcgca gcttcttcct gccagtcggt
    19441 cgggatcaga ttcatcagcg gataacgcgg catggttttg gtgcgtgaat gcgaccacga
    19501 gccagacgcc cccagctccc acgaccagtt ttccgtgcgc tcccgccaca tcaccggtat
    19561 ggcaaacgac aggtattcct gcggactgta gtaaccgccc tgaccgagtg agtagccact
    19621 cagatctttg tcgtaatgcc agatcatgtt gttcaggccg attgtgacgc ggcgattgtt
    19681 ctggttaatg accttatagt aatagcccgt catccagcgc acgcgccagt tatcttcgac
    19741 atttttaccg gttaactggt cgccactaag cgatgcccag acgccgtttg cttcaccttt
    19801 atcgtagctc agacttagcc ccacaccgtc ggcacgtacg ccaccccatt ttttcccggt
    19861 attgctcggg gagtcttttt gcccaccaaa ggccagcaaa gaactggaga tgggccgacg
    19921 gtgggcgtta acggtgtaac ccagcggccc gatatcatcg ctgtaactga tgccgccgac
    19981 cacatccacc acgttgaagc ccatcggcgt ggtaccgata tcccagctcc agacgtcatt
    20041 tcgccagccg accgccacgc tggcaccgga atccgactgg ctgcggttgc cgctacagtc
    20101 ctgtaatgta caggtgcccc agttgtcatc ccatttgcca tcggcattag tggagaaact
    20161 gccgacgttc atattgacga aatcactgcg aaagaacatc cgcccgtcag aatacggcgc
    20221 atccacctgc aacatggtag tgtgcgcttt cagatcggag taaccaccgg tgccgctcga
    20281 accccagtaa tcgtgctcga gggtgacgtt aagatcctgc tggcgataga ggtccgccgc
    20341 atcgctgcgc acgccgcgtt tcagccagtc atctttctcg tcgttacggg tcagtcgggt
    20401 gaatgtgtcg ttatcctgcg gacgcgtcgt ggtcacaccg gatgcgacca tggcgtcttt
    20461 gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct tcaaacttcg caccgtcgcg
    20521 cagcaccatc gcgctttcca tcgacggtgg ctgagatttt gcctgcggga tcaacttatt
    20581 aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc tgcgccagcg ccacgcgccg
    20641 ctgtgtgttc agcgaggcgt tatcggtagc gggcagtttc gccagctggc tacgtgccgc
    20701 cgctgtgtca ccggcagcaa tatccacttc cgtcagcccc agaatggcat cggcgttagt
    20761 tggctccagc gtcaggacat tctgatatgc agcgcgggcg gcggtgtaat cacgtcgctg
    20821 ctgcgcccag tcagccagcg tgaggtcaat acgcgtggaa ggtggttgct gacgcagcat
    20881 cgcttccgct tcagcttctt tgccgctttc tcgcaggcga ttagcggttt ccatcacctg
    20941 atcgctttgc agtcgattaa ccagctcctg aatattgctg ttccactgcg cgcgcggcag
    21001 gctgttgata tgcgccagcg ccgctctgtc ctggtcatga ccagagaggt acagcccgta
    21061 agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc agattgcgca ttaacgtatc
    21121 ggcctggctg cgttgtccgg cctgccagag atcctgcgaa agtcggtaag taatccatac
    21181 gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct gccgcctgcg cccatttgcc
    21241 ctggttttcc agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc tgcgttcgat
    21301 atcatcaatg ctacgccgct gactggcaga gagcgaggcg ataaacgctt cagctttttc
    21361 tggcgattgc tggcggtaaa tatttgccag cccgcgcacg gcgttagtgt tgccgctgtc
    21421 catacgcaag gtctgctgat aataacgttc ggcggcggga taatcttttc gcgccatcgc
    21481 cacatcgccc agccccagca ctgcataact gtcggtgtta tcgacattac gcgcctgctg
    21541 gaacaggcgt tctgcccggt caggattatt ggctttcagc gcagcatcgc cctgctggat
    21601 cgccagccag tagcggttta ctttcagcag actgttccat ttgtcgttgt tgctgctgtg
    21661 cgggtccagt gcgagggctt tttccagatt cgccactgca ttggcgcgat cgcctttctg
    21721 agaatacgcc tggcccagcg cccccagagc ttcactgtct ttcgggttcg cccgcaccgc
    21781 ctgttgtagt tcgggaatgg ctttacccgc cataccagag tccaccgccg ctaaaccttg
    21841 cgcacgagcg cggaaagcag gatcggccag ctgtttttgc tgttctgcca gttgcgattg
    21901 cgcagccgcc acgctatcgc catcactaaa gatcgagaga tattttttca gcgccgacac
    21961 actggcatca ctgacgggca tgtctttaat ctgcccgtac cagattttag aggccccttc
    22021 gcgcccggcg ttcgattttg ccatctgttc caggacggca aaaccttcgt cacgccgatc
    22081 gctactaaac agcaatagcg ccagattgtt ttgcaggccc gtattgcccg gcgtatccgc
    22141 attaatgcgt tttagctgat taatcgcttc gccacggcga gccggaattt tcgccaccgt
    22201 actccagtac tcgacggcaa tgtcaccttc cggcggcgca ccgttgaaca gtttgttgta
    22261 actcgccaca gcttcttctg catgaccggt cgtcgcctgc aatcgtgcct gttgcagtgc
    22321 ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc gacgatttat acgcattcga
    22381 actcggcgct aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa tatcgccctg
    22441 acgtaacaaa gaacggaaac gggcggcaac gacgtccggg ttattcggat caataagttc
    22501 cagccgatat aacgactgtt gcaccagatc ttcacgatgg gtcgcttcgc ctaaccgaac
    22561 ttgctccagc aactgttgct gagcggttgg tgctgcctcg accatcggca tgacggccag
    22621 accgagggaa agcgtgaata tgtttagtgt gaatttgcgc attcctggcc ccagtcaggt
    22681 aataactcac cttttgtcga gaagcggaaa cggtgttgat cccagccttg tccaaacagg
    22741 gtcagcacat agttgtaata ggcatcgctg ccgggaaagt tatcggccac gcgctggcgc
    22801 tgaacggcct gcgcatcgcg gttttgtaaa aagggcagca tggcggcaga aaaaccgacc
    22861 ggtcctttac cctgcgcttt ccccgtagcc acatccactt tttccggcgg ataaccgttt
    22921 ttctcagtga atgtcgccat cggtttaaac cggttgagca tccgcgcttt ttgcggatcg
    22981 ctgtcaggca taatgcctac ccacatgtaa acgcggatag cgtcgtagct gctgatcaat
    23041 gttttttcgg cttttagctg ccagcctttg tctttctcat agcgcaccca gtctggcgaa
    23101 aaacctttcg gggcggtttc cagcaataaa cgttgattgg tttcgcgcag cgtggtccac
    23161 ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct ggggttaaaa
    23221 cgccagctgt tatcctcagc aaaacccact ttgcccggta acaacatgga acccagccca
    23281 ggcaccgtca ccacttcctc ccgcgcgata cgttttagca acgcgctgcc gatgtcggta
    23341 taacgctgct ctttccacaa acgccccgcc tccagcaacg accaggccat ccagacatca
    23401 ccatcggagg ccgaattgct gtccagcact tcccacttac tgttctcttt cttgccccac
    23461 agccaggcgg gcaaacgttc ttttaaagag ccctgagcga gattgttctg cgtccagtcg
    23521 agaatattat cgaaagctac acggtcgtta gccgccaggg caaagaacat accgtaactt
    23581 tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg cccttcctga
    23641 ctgatgtaat cctttttaaa ctgctcccag gcaggccagg tacaggctgc ctgaacacta
    23701 aaggcagcca gcagcagcat cgtcacgatt ccactacgca acacattcat cttcaattac
    23761 tcgttatccg ggttaagacg acgacgacta ataattcgca gcagacgcca cagtacccat
    23821 gccagcaata tcacactgat agccgccagc accgccagca gaatcggatg gtttgccagc
    23881 gcataccaca cgcgctcgaa ccacggcaga tgacctacgt aataaacgtc gccaacacgt
    23941 aggctgttga taccggactc gcggatcacc gcgaccgaac cgaacatggt ggcgcgtttg
    24001 ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc gtgggctatc tgccaacagc
    24061 gcaatcacgc tgcgctggtc gttatacggc gactggaagc caatcaccgc cgccatcgca
    24121 ccggaagagg tcagcgttga ccgggtttct gccgcgcgat cgctctcgtc cggcacaatg
    24181 ccggggaacg gggtctggcg catcggtgtt ttcacccagc tttcggtcgc ctgcaccaat
    24241 aggtcgatct gcttatcgtc tttcagtttg tccgggatac caccgatgat catgatgtcg
    24301 gcatctttgc cctgaatggt gctgccatca tcggtcaccg tcagattaat cgccgggaag
    24361 cccgtctgtg cgccgataaa accaacagta ttcagcaacg tttccatctg tgcttcgtta
    24421 ggcgctttcg gcattacggt gatggtttgc gacagatccg ccatccggct gaatgggaag
    24481 cccgcgttag caaaggcgcg tagatccggc atcgggatga agtggtaata cttcgagaag
    24541 tcgatggtgg agtcgtcacc aatcaccaca tgattctgca ccggctggaa ggtaatacag
    24601 ttatccaccg aaccgcccgg catcgggttc atatactcaa agtcgaagcg cagctggttg
    24661 gtcgcgccca gtttcagcgc cggaatagag acatctgttt tgccatccag caaaccttgt
    24721 aataccggaa tccgcagcag caggcggttc gcctcctgtt tgctgctcag gttgaaggat
    24781 tgcaggaact ggttattcag gctgatatcc atccgcgaac tgtctttcac cggcggcatg
    24841 gtgtagcggt aattaatatc catatcaatg ccggtactgc gcatcaggta gagatccggc
    24901 ggcaggttta gcgaaacgtt aatcgctgct ggctcaagac cgctggattg taactgttct
    24961 tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg tacgtaccca gttcggcgca
    25021 tcgtacggct tacgcggtag cagcggtttc acttcattca ctaccacgct ttcaccacgg
    25081 aacagaatgt taccctgagc gatacctttc gctgcctgca acaggtcttt gtcgtcacga
    25141 ccaaacacca ccagcagttt gacgtaagga ttctgcggat ggttaatcat ctcaatcacc
    25201 ggggctttta ccgccggatg atcgcgcagg aagtccggcc gtttgtcgtt ggttgcaaag
    25261 acaatggcat tacgatccgg cagttggtta tagagcaccg ggaagttctg cccacgccag
    25321 ccagaacgcg aaccaaacca cgaggcgaca atggcagagg cttgttgcag cccaacatcc
    25381 ggcgcacccg caaagaccat cggcaaggtg ttggtgcggt tatcacgcgg atcaaagaat
    25441 ggcaccggga agtgtgacag gtcattcttc acattcaggg tctgataggt cagatccagt
    25501 ccactgctcc gcccaacatc cagccaaagc gtggtgctgg ccgggttttc gcacacgtcc
    25561 tgataatggc cgacaaactc cagccgcaca cggttgaagt cggtaataaa cagtgggtta
    25621 atgggcattt gcgccagcgt ttttttaccc aactgttctt tggtcactgg cagcacgccc
    25681 atcagctcat cattgagata aacctttaac tgcgactgga caggcagtaa cgatggcgat
    25741 ggcgtgtatt cgaggttgag catcgccttc gtcaccactt catcgctgcg cataccaaac
    25801 tcaatgctgc cgttcgggtt aatgccacgt agcaccatgc tgcccggcgg cggcgcaatt
    25861 tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct gcgccacgac tggcgcatca
    25921 gcgccctgca cgcccggcat cacttgcccc acctgcggat tttgttccgt ctgggcggct
    25981 acagctggct cagcattgat cagtggttgc gttgctggcg tcgcctgcgt catgaaagag
    26041 gggaacgcac tcatccccat agccactgca caaatccaga atagttttct tttcatcgcg
    26101 ttatcatcat tgttgagcca aagcctgatc cgatggttgt gccgtttcgc tccgctccgg
    26161 gcggcgcgga ataaacgata caacccagga aaccagagaa gtcagcacac ggaatatgcc
    26221 cttcaccgaa gaaggcgcaa actccgccag atggcggtag ccacggaagc cgagcttcag
    26281 aatatccagc agactttcca gcggcttatc ttccgggtag ctgtcctgcc agagcgccca
    26341 tgtatccgca cgggcaaacg tacactgcac aaaatcgata tgttgctggg tggtgagcgg
    26401 cattaatttc agcccaactt cattacccat cacgcgcgcc acctgggtcg ggaagacgta
    26461 ttcctgctga ccgcgtttaa gcaacagatt cactttctgc ccttccagaa tctgcgcctg
    26521 accgttgatc ttgatcccca aaccaccgtc ggagaaatcc tgaacggtac acgagaagag
    26581 gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc tccacgcggt gcgatcggcg
    26641 tacctgtttg ctttctaccg ataccgcaac tgcgccgcca agaacaatca ggttgtagaa
    26701 cacccacacc atactgacga ccacggtgag catctcggtt ggcgggccat agaagtagcg
    26761 ccagatgcct accgcaacgc ccaccaggtt gagtagaaca aggaagatgt agggccgcga
    26821 gatcacccag tcgacgtact cttcttccac cagtccacct ttggcggtga cgttaaattt
    26881 gcctttgtgc gggttaatca gcgccaccag cgtcggtggt gcgatatacc acgccagcac
    26941 cgtttcgtag atttcactcc agaaagagtg gcgatatttg ccctggatct tcgagttggt
    27001 caggctggca tggatcatat gcggcagcac gaatagggcg atcatcaacg ctggcgcata
    27061 gatgatgtag gcatgaagca gcaggaacgc cagcggcgca gtcaggaaga tcagccgtgg
    27121 aatgcccgac aagaagtgga acatggcgtt gacgtagcac agccgctgag caaacttcag
    27181 tcctttaccg gtgagcgggt tatcgagacg gaagatttgc accatcccgc gcgcccagcg
    27241 aatacgctga ccgatatgcg ccgacagact ttcggtcgcc agccccgccg cctgcggaat
    27301 acgcatatac gcggaggtat atccacgacg gtgcaaacgc agagaagtat gcgcatcttc
    27361 agtcacagtt tcgacagcaa tgccgccaat ttcatccagc ggcttacggc gaatcaccgc
    27421 acaggaaccg cagaagaaag tggcgtccca catatcgttg ccatcctgca ccagaccata
    27481 gaacagcgtg ccttcgttcg gtgttttacg aaaacgcccc aggttgcgtt caaacgggtc
    27541 tggcgagaag aagtggtgtg gagtctgcat catcgccagc tgtttctctt tcaggaacca
    27601 gcccatggtc atttgcagga acgatcgcgt tggtacgtgg tcgcagtcga aaatcgacac
    27661 gaactcgcct ttggcatatt tcagcgcatt gttgatgttg cccgctttcg catgttcatg
    27721 agtggtacgg gcgatatatt tcacccccac gttttgcgca aactggcgaa actcttccct
    27781 accgccgtca tcgaggatcc agatgttcag cttgtctttc ggccagtcga tacccagcga
    27841 ggcgtaaata gtatttttca ccacgttgag atcttcgttg taagtcggga caaagatatc
    27901 caccgacggc cacagcgaca tatctttcgg caatggcacc ggctgacgat tcagcggcca
    27961 tactacctgg aagtagccga gcaccagcac aatccacgcg tacgtttcag cgaagagcag
    28021 aataagcccg cacaccaggc tgaccggatc gtcccagttc agcgtagagg tgtaacgcca
    28081 ccagatataa cggcaagaaa cggtcagcga cagcacaatc aacattagcg ccgagaagcg
    28141 ccccggcatc cgccgtacga tcagcgctac accccacagc agcatcagga agataaactg
    28201 cgccagcggg ttaaacggtt gagtaacgca gattaacgcc agaatcagcg agaaggtgac
    28261 gatgataccg aggatcaacc gccgcgcccc ggcactcaaa tgaccgagct cttttttctc
    28321 atcaagatgc tgtgttttat ggctaacgcg ctcaggcagc tcgttcatcc attgatggta
    28381 acgtccacga atattctgca gacctgaaaa tgcccgcctg cgcggtttcg gcgtttcttt
    28441 gcgcgatgta ccgatcagta accagcatgt ttgaatgaga taacggaccg ggtccagcgg
    28501 acgcggacgc gaggcgttga tatgcggata caggttttta tgttctgcgc gaatacgctg
    28561 ccagcgcggg tgctccagcg gaataaaaat ccaggccagg atcatccaga aacagccgag
    28621 cgtcgcgctg aaagccgacg caccgtgacg acgataatcg cgataacgcc cgataagccg
    28681 cgcgttgacc ggcgggataa gcaaccaccg ggtcaggata ctcatgatgc actcccgact
    28741 ggcgttttca gcccggagta gttcaacagg caccagttcg ccagcgtcag tatctcttca
    28801 gccgccagcg catcactgcg atattcacct actggttgct tagccgccag gcattcagcc
    28861 atcgcttcat cacgatgaat gagcatcggc agtaatcggc gctggctttg caaccaaagc
    28921 tggtaaatat cgtcctgaac ctgactgcca atacggaagt tattaatcaa aatatgtgcg
    28981 ccatccggca gcgcttgctg atgcagtcgg atatggcagt tggcatccac attgacgatt
    29041 gccagcgagt gatcgcacaa actcagcagc tggtgggtta tctgcgaggc atcacgcggt
    29101 aagtcgatta aaatccactg gtaacgcccg ctggctttta gttgctgtaa gccggagcaa
    29161 atatcgctca gccgggtttg ccagtgctgt ggattttctt gttcttcaat ggataactga
    29221 ccaaaaggca gcaaatcgag ctgcgaggtg tagcgcaacc cagcgtcacg ccagtcctgg
    29281 ccatccagca tcgctctggc ccagccctga cggtgggtaa aatcaacatt aaatgacagg
    29341 cgcaacaagt tgtccgggca ggcatcgacc accagaacat tttctcccaa catttgtaat
    29401 gaccaggcta atgcggcggt gatggttgtt gtccccacgc ctccccgcac cccctgcaat
    29461 cccagtacgg ccattcacca atcccctact tttgttgcgc aaactctgcc agcaacggcc
    29521 agcgttttaa tgccgcggcc aactgttcgc gttgggaaat atcggcataa tcaatatcag
    29581 gcagtgaaaa tgcctgcttt aacgccacaa tatcattctg gaagatatag cctatcgcgg
    29641 gatcaggcag agtatctggt tcattgttat tcattttgct tgatccctat gaaataaccg
    29701 taccgttcat gagacaattc taaccgtgag catttcgccg gtttattgtt tacggcgaga
    29761 cgggaatttg ctcacttttt gactttccgt tacgcaacag aaaaagttaa aaacgaaaag
    29821 actaaaatca cacgtcttgt gaccttctaa attgattcac atgctaaatc tgataagttt
    29881 taatttcaat ggtaggttta tttcttagct ttcgctagta aactgataat cagacaaaat
    29941 ggtgacatga gggacattgt ggaccctgta ttctctatcg gtatctcatc attatgggat
    30001 gagctgcgac atatgccagc aggcggcgtc tggtggttta acgtcgatcg ccatgaagat
    30061 gctatcagtc tggcgaatca aacaattgca tcccaggctg aaaccgcaca cgtcgcggtc
    30121 attagcatgg acagcgatcc ggcgaaaatc tttcaattag atgattctca agggccggaa
    30181 aaaataaaat tattttcaat gctaaatcat gaaaaaggtc tatactattt gacccgtgat
    30241 ttgcagtgtt ctattgatcc ccataattac ctttttattc ttgtttgcgc aaataacgca
    30301 tggcaaaaca ttcctgccga gcggcttcgc tcatggttgg ataaaatgaa taaatggagc
    30361 aggttaaacc attgttcgct tttggtaatt aatcccggaa ataataacga taaacaattt
    30421 tcattgttgc ttgaggaata ccgttcactt tttggtcttg ccagtttgcg ttttcagggt
    30481 gaccaacatt tgctggatat tgccttctgg tgcaacgaaa aaggggtcag cgcccgtcag
    30541 cagcttagcg ttcagcaaca aaatggtatc tggacattag ttcaaagcga agaggcggag
    30601 atccaaccac gcagcgacga aaaacgcatt ctgagtaatg ttgctgtact ggaaggtgcg
    30661 ccgccgctat cggaacactg gcaactgttc aacaataacg aagtcctgtt caatgaagcc
    30721 cgtaccgctc aggcggcgac ggtggtcttt tctttacagc aaaatgcgca aatcgagcca
    30781 ctggcccgca gcattcatac cctgcgtcgc cagcgcggta gtgcgatgaa aatcctcgtg
    30841 cgggaaaata ccgctagcct gcgcgccacc gatgaacgtt tgttattggc ctgcggtgca
    30901 aatatggtta ttccgtggaa tgcgccactc tcccgttgtc tgacgatgat cgaaagcgtg
    30961 caagggcaga agtttagtcg ctatgtgccg gaagatatca ctaccttgct gtcaatgacc
    31021 cagccgctca aactgcgtgg tttccagaag tgggatgtgt tctgtaatgc cgtcaacaac
    31081 atgatgaata accctctatt acctgcccac ggtaaaggcg ttctggttgc cctacgtccg
    31141 gtaccgggta tccgcgttga acaagccctg acgctgtgtc gccctaaccg taccggcgat
    31201 atcatgacca ttggcggtaa tcggctggtg ctgtttctct cattctgtcg gattaacgat
    31261 ctggataccg cgttgaatca tattttccca ttgcctactg gcgacatttt ctcaaaccgt
    31321 atggtctggt ttgaagatga tcaaatcagt gccgagctgg tgcagatgcg cttgcttgcc
    31381 ccagaacaat ggggcatgcc gctgccttta acgcaaagtt ctaaaccggt catcaatgcc
    31441 gagcacgatg gtcgccactg gcgacgaata ccagaaccca tgcgactgtt agatgatgct
    31501 gtggagcgct catcatgatg accatcagcg atatcattga aattattgtc gtttgcgcac
    31561 tgatattttt cccgctgggc tatctggcgc ggcactcttt gcgacgcatt cgcgacacct
    31621 tacgtttgtt ctttgctaaa cctcgttatg ttaaaccggc cgggacgtta cgccgcacgg
    31681 aaaaagccag ggcaaccaaa aaatgactca atttacgcaa aataccgcca tgccttcttc
    31741 cctctggcaa tactggcgcg gcctttccgg ctggaacttc tattttctgg ttaagttcgg
    31801 cctgttgtgg gcgggatatc ttaacttcca tccgctcctc aatttggtgt ttgccgcgtt
    31861 tctgctgatg cccattccgc gctacagcct gcatcgcttg cgccactgga ttgccctgcc
    31921 gatcggcttt gctttgttct ggcatgacac ctggttgcct ggcccggaaa gcataatgag
    31981 ccagggttcg caggtggcgg ggttcagtac cgattattta atcgaccttg tcacacgctt
    32041 tattaactgg cagatgattg gggccatttt tgttttatta gtggcctggt tattcctgtc
    32101 acaatggatt cgcattaccg tttttgtggt tgccatactg ctatggctga acgtacttac
    32161 cctggcggga ccaagtttct ccttgtggcc agccggacaa ccgacgacca ctgtaacaac
    32221 gacgggtggt aacgcagcgg caaccgttgc ggcgacgggt ggcgcaccgg tagtgggtga
    32281 tatgcccgca caaactgcac cgccaacaac ggcgaacctt aacgcctggc tgaataattt
    32341 ctataacgcg gaggcaaaac gtaaatcgac cttcccgtct tcgctgcccg ctgatgctca
    32401 gccatttgaa ctactggtga ttaacatctg ttcgctttcc tggtcggata tagaagccgc
    32461 cgggttgatg tcgcatccac tgtggtcgca tttcgatatt gagttcaaga actttaactc
    32521 cgccacctcc tacagtggcc cggcggcgat ccgtttactg cgcgccagct gcgggcagac
    32581 ttcgcacact aatctgtatc aaccggcaaa taacgactgc tatctgtttg ataacctttc
    32641 gaaactgggc tttacccagc acctgatgat ggggcataac ggccagttcg gcggtttttt
    32701 gaaagaagtt cgcgaaaatg gcggcatgca gactgaattg atggatcaaa caaatctgcc
    32761 ggttattttg ctgggctttg atggttcgcc ggtttatgac gataccgccg tgcttaaccg
    32821 ctggctggac gttaccgaaa aagataaaaa cagccgtagt gccacgttct acaacacgct
    32881 tccactgcat gacggcaacc attatccggg ggtcagcaaa acagcggatt acaaagcgcg
    32941 ggcgcagaaa ttctttgatg aactggacgc cttctttact gaactggaga aatcgggtcg
    33001 taaagtgatg gtggtcgtgg tgccggaaca cggcggcgcg ctgaagggcg acagaatgca
    33061 ggtatctggc ctacgtgata tccctagccc gtctatcaca gacgtccccg ttggggtgaa
    33121 attcttcggc atgaaggcac cacatcaggg ggcaccgatt gtcattgacc aaccgagcag
    33181 cttcctggct atctccgatc tggtggttcg cgttcttgat ggcaagattt tcaccgaaga
    33241 caatgttgac tggaaaaaac tcaccagtgg gttgccacaa acagcaccgg tctccgagaa
    33301 ctcaaatgca gtagttattc aataccagga taaaccgtac gttcgcctga acggcggcga
    33361 ctgggtgcct tacccgcagt aagcacgaaa aaggccgcag agtttaacct ctgcggcctg
    33421 atccgggcgc aaattgccat tacggcagcc tgacgcccgc atgacacgtt acttccgctt
    33481 attcagccag ttcacgatca tactggcaag aatgccagca atgaccggag ccgctaaatc
    33541 atgccagaag gccatgccca gttctgcgag cgtcatatag ccgcctgtgt tgtaatgaca
    33601 acgtttcgcg gctattcttg agttgttcag gttcaagaat ggcccccgtg atgttgtcag
    33661 gtgcatacct gcaacgtgcg ggggttttct ctctccagca accaatgcca ccagggataa
    33721 agcccccgca acattgcgcc tcaccggaac ccttccggct tgacgctgat tctacgactg
    33781 tttttctgtt ccggaatcca tcacttactc actctgtgct gcgcattccg cagaatatta
    33841 tccgcttgca accgttgcat ttttcttacg aaaaaggccg cagagtttca cccctgcggc
    33901 ctgatccggg cgcaaattgc cattacggca gcctgacgcc cgcatggcac gttacttccg
    33961 cttgttcagc cagttcacga tcatactggc aagaatacca gcaatgaccg gagccgctaa
    34021 atcatgccag aaggccatgc ccagttctgc gagcgtcata tagccgcctg tattgtaatg
    34081 acaacgtttc gcggctattc ttgtattgtt tggatgcaag aatggccccc gtggtgttgt
    34141 caggtgcata cctgcaacgt gcgggggttt tctctctcca gcaaccaatg ccaccaggga
    34201 ttaagccccc gcaacattgc gcctcaccgg aatccttccg gcttgacgct gattctacga
    34261 ctgtttttct gttccggaat ccgtctcatc ttcactctgt gctgcgcatt ccgcagaata
    34321 ttatccgctt gcaaccgttg catttttctt acgaaaaagg ccgcagagtt tcactcctgc
    34381 ggcctggtcc gggcgcaaat tgccattacg gcagcctgac gcccacatga cacgctactt
    34441 ccgcttgttc agccagttca cgatcatact ggcaagaatg ccagcaatga ccggagccgc
    34501 taaatcatgc cagaaggcca tgcccagctc tgcgagcgtc atatagccgc ctgtgttgta
    34561 atgacaacgt ttcgcggcta ttcttgagtg gtctaggttc aagattagcc cccgttatgt
    34621 tgtcaggtat atacctgcaa cgtgcggggg ttttctctct ccagcaacca atgccaccag
    34681 ggataaagcc cccgcaacat tgcgcctcac cggaaccctt ccggcttgcc gctgatttta
    34741 cgattgcttt ttactgtcag cacccggcaa tcttcctcat tgcgtgatgc cttccagctt
    34801 aatttcttaa tttgcaatag ttgcagttca tatccgggct gactccagac tggtagtgaa
    34861 atgcgcaatt ttgtagaatt gcgcctcctc gcgacctccc cagtgaatct gttgcctcat
    34921 tttttacata ctgcgtattc gacttctcca cctgttgcgc aagagaatct gggtttattc
    34981 atttttgcga ggccgacttc tttctggaca ggactttatg cagcacaaca cactaccgaa
    35041 acacgatcag aaattgccgt ttacacgcta cgacttcggc tgggttttat tatgcatagg
    35101 catggcgatt ggtgccggaa ccgtgctgat gccagtacaa attggcttga agggaatttg
    35161 ggtatttatt accgcagcga tcattgctta tcctgccact tgggtagtgc aggacattta
    35221 tttaaaaacc ctttctgaaa gcgattcctg taatgactac accgatatta tcagtcatta
    35281 cctggggaag aactggggaa ttttcctcgg ggttatctac tttttgatga ttatccacgg
    35341 gatttttatc tactctctct ccgtggtttt cgacagcgcc tcgtacctga aaaccttcgg
    35401 tttaaccgat gccgatcttt cacaatctct actttataaa gtcgctattt tcgccgtact
    35461 ggtggcgatt gcgtctggtg gtgaacgatt actgtttaag atttctgggc caatggtggt
    35521 ggtcaaagta gggattattg tcgtgttcgg ttttgcgatg atcccgcact ggaatttcgc
    35581 caatataacc gccttcccgc aagcctccgt ctttttccgc gatgtcctgc ttaccattcc
    35641 cttttgcttc ttttctgcgg tatttattca ggtacttaac ccaatgaata ttgcctatcg
    35701 taaacgggaa gcggataaag tactggcaac ccggctcgcg ctgcgtaccc accgaattag
    35761 ttatgtcacg ctcatcgcgg tgatactgtt ttttgccttt tcgtttacct tctcaattag
    35821 ccacgaagaa gccgtttctg cctttgaaca aaatatctcg gcactggcac tggccgcgca
    35881 ggtgatccct gggcatatca ttcatatcac ctctacggta ctgaatatct ttgccgtact
    35941 gaccgcattc tttggcattt atctcggttt ccacgaggcc attaaaggca ttattctcaa
    36001 tctgttaagc cgtattattg ataccaagaa aattaactca cgcgtgctga ctctggcgat
    36061 ctgcgctttt atcgtcatta cgttgacgat ttgggtttcg tttcgtgtat cggtgctggt
    36121 gttctttcag ttgggaagcc cgttatatgg cattgtgtcg tgcctcattc cgtttttcct
    36181 gatctataaa gtcgcacaac tggaaaaact tcgcggattt aaagcctggc tgattctgct
    36241 gtacggcatt ttgctatgct tgtcgccact gttgaagctg attgagtaaa ccggagcaca
    36301 aggctccggt tttgaacatt accgctgcgg attttcatcc tgatcaacgg caaagcaggc
    36361 caccagttga ccgccgtaat ctttcagtgc tggctgcaac tgggtgcagg ggccgaagcg
    36421 tcgacgacag cgggcgttga aggcgcaacc cggcggagga ttcagcgggc ttggcagttc
    36481 accggtgagc ttgatgcgct cgcggcgatc gtccgggttc aggcgcggcg tcgcggagag
    36541 cagcgcctga gtgtaaggat gacgcgggtt attgaagatt tggtccttcg tccccttctc
    36601 cacgcagcgg cccaggtaca tcaccatcac ttcatcggca atgtgctcca caaccgacag
    36661 gtcgtgcgag ataaagacat aagaaagccc cagttcctgc tgcaaatcca tcatcaggtt
    36721 gagcacctgc gcgcgcaccg agacgtccag cgcagaaacc ggttcatcgg caatcaccac
    36781 gtccgggtcg agcatcagac cacgggcaat agcgatacgc tgacgttgtc cgccggaaaa
    36841 catgtgcgga tagcggtcgt aatgctcggt tttcaggccg actttcgcca tcatcgacag
    36901 ggctttttcc cgacgctgtt ctttgcttaa gctggtgtta ataagcagcg gctcttcgag
    36961 aatttgccca acttttttac gcggattcag cgaaccgtaa gggttctgga agacgatctg
    37021 gattttctgc cgacgcagct tctgcgcctg cggatcgtgc ttaagcagat cctgcccctg
    37081 gtaatacagc tcgccaccgg tgggcatttc aatcatcgtc agcaaccgac cgagggtcga
    37141 tttaccgcag ccagattcgc ccactactgc cagcgttttg ccacgttccg gcgcgaacat
    37201 gcctttcttc accggataat gttttttcag gtcgatagcc tgcaacagcg gttgttgcga
    37261 ggtggcctct tgcgtactca tagtgtcggc ctcccggcat catcaagtgg gtaatggcat
    37321 ttggactgac gcccatcagc gagcatattc agcgccggtt cttcagcgcg acatctgtca
    37381 gtggcatagg ggcagcgcgg gttaagcagg cagccgttcg ggcggtcgta cttaccggga
    37441 acgacacctg gcaacgacgc cagacgttct ttgtcctgag caaattctgg cagcgcacgc
    37501 agcaatgcct gagtatacgg gtgacgcggc gcatggaaga tggcgtgcgc atcaccggtt
    37561 tccaccacct ggcctgcata catcacgatg attttatgtg ccgcttccgc caccagcgcc
    37621 aggtcatggg taattaacac cagcgccatg ttctctttct gctgtagctc cagcagtagt
    37681 tcgatgattt gcgcctgaat ggtcacgtcc agcgcggtgg tcggttcatc ggcaatcagc
    37741 agttttggcc gacaggcaat cgccatggcg atcatcacgc gctggctcat gccgccggaa
    37801 agctgatgcg ggtaaacatc cagacgcgat gccggatcgg gaataccgac ctgattcagc
    37861 aggtcaatcg ctcgctgacg gcgggtactt ttgttgccgc cctgatgcac cttaatcgct
    37921 tccataatct ggaaacccac ggtgtagcac gggttaaggc tggtcatcgg gtcctggaag
    37981 atcatcgcca cttcggcacc caccaggttg cggcgctctt tttctgagat acgctgcaaa
    38041 tcctggccgt taaactccag tttttctgcc attacgcggc ccggataatc aatcagcccc
    38101 ataatcgcca gtgaactgac cgacttaccg gagccggact cacccacaat cccgaccact
    38161 tcgccctgtt ttacgctgta gctgatgcgg tctacggcgc ggaacggcgc gctttcgtcg
    38221 ccgaaatgca ccgataattt atctacattt aataacgcca tctcgaacct cttactgctt
    38281 cagtttggga tcgagcgcgt cacgcagacc gtcacccatc aggttgaatg ccagcaccgt
    38341 cagcaggatc gccagacccg ggaaggtcac gacccaccag gcgctttgcg cgaactgcaa
    38401 cacgtcggag agcatggtgc cccactcagg cgttggcggc tgtgccccca tgccgaggaa
    38461 accaagagca gccatatcga gaatggcgtt agagaaaccg agcgacgcct gaacaatcag
    38521 cggcgcaagg cagttcggga agatgttaat aaacatctga cgcatcgccc cggcacccgc
    38581 cacgcgagac gcggtgacgt aatcgcggtt aacttccacc agcacggcgg cgcgggttaa
    38641 gcgcacatag tgcggcaagg caacgaaggt cagtgccagc gcggcgttac caatcgacgg
    38701 gccgaaaatt gccaccagca ccagcgccag cagcagactt ggcagcgcca gcatgatatc
    38761 gaccacgcgc ataatgatgt tatcgaccag gccgccaaag taaccggcga tcaggccgag
    38821 aataacgccc ataatcagcg ataacacaac taccagacag ccaaccagca gcgacaggcg
    38881 cgcaccgtac atcaggcgcg acagcacatc acggcctacg tcatcggtgc ccagcaagtg
    38941 agccatgctg ccgccttcct gccaggctgg cggggcgagc agtgcatcgc ggaactgttc
    39001 cgccgggtta tagggtgcaa tccagttggc aaagatcgcg atgaacagca cgatgacgac
    39061 gtaaaccagc ccgacgaccg cgcctttgtt gcgtttaaaa tagtgccaga actcctgtaa
    39121 cggggtcatc ggcaccggtg cgctaatcac tttattttca gtaacctgtg acatgatgtc
    39181 cccttacttc ttatgacgaa tacgcgggtt caccacgccg tacagcagat cgaccagcaa
    39241 gttgacgagg ataatcatcg tcgccaccag caatacgccg ccctgcacta ccggatagtc
    39301 gcggcgttgc agtgcgtcaa tcaaccagcg tcccagaccg ggccacgaga agatggtttc
    39361 ggtcagaatc gcccccgcca gcaatgttcc cacctgcagg ccgataacgg tcaccaccgg
    39421 cagcatcgcg ttacgcagcg catggacgat aatcacccgc atgcgggtta gccctttggc
    39481 gcgcgcggtg cggatgtaat cctcgcccag cacttccagc atcgaggagc gtgtcatacg
    39541 cacaatgacc gccagcggaa tagtacccag cacaatggca ggcaagatca tatgggcgac
    39601 ggcatcgata aagttgccgt cttcacccca gatggcggtg tcgattagca taaaaccggt
    39661 taacggattg gagtcatcga ggaacaccat atcgctcacg cgaccggaga cgggcgtcag
    39721 gttccagtgc accgaaacca gcatgatcag catcatgccc caccagaaga tcggcatcga
    39781 gtagccggtc agcgccaggc caactgcagt gtgatcgaaa atggaaccgc gtttaaccgc
    39841 agccagcaca ccaaccggaa taccgactgc cgtagcaaaa atcatcgcgc agacgccaag
    39901 ttccagcgtg gcctgaaagc gcggcacgaa ctcttcccat actggaattc ggcttttcat
    39961 tgaaatgcct agatcgctgt gcattacgcc ccaaatgtaa tggagatact gctgccacat
    40021 cggtttatct aagccgagtt cagccagcag ctgcgcgtga cgctcagggg agatcccacg
    40081 ttcgcccgcc atgatcatca ccggatcgcc cgggatcatg tggacaaagg caaatgtgag
    40141 aagggtaata ccgataaacg tggggatgac gagtcccaaa cgtcggagaa taaactgcaa
    40201 cataacccgg attctctata atgacccgca gcctggaact gcgtgtctgt attgctcaca
    40261 aatcaattcc cgcccttgtc ggacagagaa tgaagtattg ctgccggatg gcgctgcacg
    40321 cagaggcaca gcgccaccgg gtatggcttt taattattcg atagagacgt tttcgaagtg
    40381 atgtttgcct aatggatcaa ccacatagcc tttaacttct ttacgtaccg gttcaaacac
    40441 ggtggagtga gcgatgatca gtgccggagc ctgatcgtgc atcaccacct gtgcttgttt
    40501 gtacagttca acgcgtttgt tatggtcgtc ggtagcacgc gccggttgaa tcagatcttc
    40561 aaacggtttg tagcaccatt ttgagtagtt ggagccttgt tcagaggcgg cgcagctgaa
    40621 cagggtggcg aagaagttat ccggatcccc gttatcgcca gtccagccca tcattaccgt
    40681 ctggtgctcg ccatctttcg cacgcttgag gtactcaccc cattcgtagg tgacaatttt
    40741 ggcctgcacg ccgactttcg cccagtctgc ctgaatcatc tccgccatgc ggcgagcgtt
    40801 cgggttatac ggacgttgta ccggcatcgc ccacaggtcg atggagaaac ctttttccag
    40861 acccgcttct ttcagcaagg ctttcgcttt ttcaggatcg taggtgtagt cctgaacgtc
    40921 gtcgttatag ccccacatgg ttggcgggat caggtttttc gctgatacac ccgcgccctg
    40981 ataaaccgct ttgatgatcg cgtctttgtt caccgcgtag gtcagagcct ggcgaacttt
    41041 tacgtcatcg agtggttttt tctgcacgtt atatgaaaga tagccgacgt tcagccccgg
    41101 catttccatc agattgatgg atttatcctg cttcatgcga gcgatatctg ccgggttcgg
    41161 gtacggcatc acctggcatt cattcttctg caatttcgcg taacgcacgg aagcgtcagg
    41221 ggtaatagag aagaccagcg tatcgatctg cggtttggtg ccccagtagc catcaaacgc
    41281 tttgtagcgg atacgggaat ctttttgata ctgctgtaac tggaacggac cggtgccgat
    41341 tgggttgagg tccagttttt ccggtgtacc ggctttcatc atcgcatcag catattcttt
    41401 tgacagaata gaggcgaagt ccattgccag gtcagcgagg aacggcgctt ccgggcgagt
    41461 cagcacaaac tgaactgtgt tgtcgtccac ctttttcact tcgctgatca gctccggcaa
    41521 gcccatgcct tcgaagtatt cgtagctgcc gccagaaact ttatggtacg ggttttgcgc
    41581 gtttttctga cgatcgaacg agaacaccac gtcatcggcg ttcagttcac gcgtcggttt
    41641 gaattcttta ttgtcgtgcc acttcacacc tttacgcaga tggaaggtat aggttttacc
    41701 gtcttcgctg acttcccact tttcagcgag gcccggaatc acttcggtgg tgccgatttt
    41761 aaattcaacc aggcggttat aaagcggtac ggaagaggcg tcataggtgg taccggaggt
    41821 aaacagctgc gggttaaacc cttccggaga tccttctgag caataaacca gagttttagc
    41881 ctgaacactt gctgcgacgg tcatagccac caggctgaga ccaagcttca gcatccctga
    41941 ctttttcaag gaaatacgca ttattctgct ccaattgtga tgtttgttgt tttaaccctt
    42001 tgcagtgggt tgtcgctgcc tgaccttttt tgttttttgc ccggtcgggt caacgttatg
    42061 aggtggggat gccgtactaa ttaacatcag tgtaacagtg cggatcctcc ataaaatgcc
    42121 cctcgtgacc tacaatctgt caacagaatg tgaaaacgtc aatacagcca accgggattt
    42181 acaccaacgg tgagaatcca caaacaaaga ttaaaaaaac ttcaaacagc tatttgcagc
    42241 agagaaattt gtgctactcc aacatgacca gaacaatcag cttaatattt agcaacattt
    42301 ggtgattaag ttttatgcat ttagtgaaaa aaatctgagg aaaaggtgga tatctgagcg
    42361 attaatacca tgaacgctaa aacgcacagc ggaaaatgcc agcgccagcc ataagttacg
    42421 aaaaaaaaga tttaagcaaa tataaaaaaa gacaatggtt tctgtcacaa atccgttaat
    42481 ggatagtgag atatggggcg caaatttggc agggaaatgt gtggcgtgta ggtcgaataa
    42541 ggcgttcacg ccgcatccga caataagtgc ggtgccgttg cctgatgcga cgctgacgcg
    42601 tcttatcagg cctacgtcga gtcgaacctc cccgcccccg gaggttctca tcctcatgag
    42661 ctacaaatgc aaaaaagcct gctcgttgag caggcttttc gaatttggtc ggtgatagag
    42721 gattactcgc cacttcgtgg ctcgccctgc gggccgttgc tggcgcaacg ttctctcgct
    42781 tcgctcgagt cgaacctccc cgcccccgga ggttctcatc ctcatgggct tcaaatgcaa
    42841 aaaagcctgc tcgctgagca ggcttttcga atttggtcgg tgatagagga ttcgaacctc
    42901 cgaccccttc gtcccgaacg aagtgcgcta ccaggctgcg ccaatcaccg aatgcggggc
    42961 gcatcttact gcgcagatac gccctcgtca atcccttaat agcaaaatgc cttttgatcg
    43021 gcgagaaagt cagcaggccg cttagttagc cgctgccact tttgtctgcg ggatgtgaca
    43081 ccagttgttg ttttcgttaa ttccaccatc cggtgaagta tagccaagac aacccatgat
    43141 ggtgtcgtac agctctacat gacggcgtgg cactttcatg tcggcttctt ttttcagctg
    43201 cgcaaatgcc tgagcattgg ccggattttc cagatattta tctgacatcc agaccatcat
    43261 cggtacgcgg aactgctccg gcggtgccag ttcacgcggc gtgccgtgca ggtgctcgcg
    43321 ttcattaatt gactcaccgt ggtcagctgc gtagaacaca atcgctttct tatcgcgaac
    43381 ctgatcaatc acgctggaga taaagtgatc cacataggtc accgagttgt cataggagtt
    43441 gatcatctgc gctttggtac agccgctatc aacaccaata cattccggct tccactgcgc
    43501 gaagctacgc ggataacgct gggtgtagtt aaaatgcgaa cctttggtat gcagaatgat
    43561 cagatgctta ccatccgggt tgcgccctag cgattgctgc atttcgtcta ccagcaacat
    43621 atcatctacc ggcttgccac gattacgtgg ctccgcacca atctgctcac gataagcaat
    43681 gttgtccgcc atcgtgttgc tgtagaacca catttcgctc tgcatagcgt agaggtcaga
    43741 actgaatcct aactgcttca gaaccgcgaa aatgttctgt tcttttaatg tgcgctgcgg
    43801 attatcttcc gcgcccccct gacgtacaaa catgcaacgc agtgagagtt tggttgcggt
    43861 atcacaggag taaccacgga acgccgccag atttttctcc tgggccagtt tcggcgtggt
    43921 attacgctca tagccgaaaa tgcccatatg gtcccaacgc gtggtttcac cgatgataaa
    43981 cacgacataa gtgtcatcaa cgttttgcgg tgcctgataa gtgaatttct tcgccggatt
    44041 aagcaatgaa ttattatcgg aagattcatc gacccgcgcc caggcataca gccccaacgc
    44101 agaaagccag tttgatggca gataagagtt cgctacgaca ccgccataac tcggcaaatc
    44161 aacgccggtc gccctctcca ctttcttctg ctggatatcc agcaaacgaa tcggtgccca
    44221 aaccataata cccgccagta cgacgaccgc caggctgcga atacgctgcc ctggggttcg
    44281 cagttgtcgg agcaaggtgt agcgacagcg gttattccag ataaggataa gaggcaatgc
    44341 actaacggcg attaaccaga gaataaagtt cagaccaaca acttcttttg acaggtcgat
    44401 atcggtggtc atgacggaag cgatgatgcc ataaccaatg accacattaa ggaaggtcat
    44461 gtaatagctg gcacctgcgg aaaagagcac caccagcgac gccagaatac gccagctgcg
    44521 gcggccaaac agcgatagaa gacgtagtaa aaagaaggtc accagtacgg tggcggccag
    44581 ttcaacaaca gcagaaacgc ctttccagac ggtaaaatcg tgcgcatagc tgccgaagcg
    44641 gcgataaaaa accgcgccat tcataaaaag gccaatatag attgcaagca aaaagctcag
    44701 cttctgctgt gtaatcgatt tgatgtatct catgcaaaca aaccctggac aaacaggtga
    44761 taacaccctg cttagcggct tttcaggccc ggcaggggaa aagtgcggag gtagtcagat
    44821 aagtaaaatc tacacgcgcg aataagtaga ccacagcaaa gcgaaaaagt gtggggaaag
    44881 agtgtgcatg aagcacaaat ttacaaaaat gcaagtttga aaagagactt tagcgttaca
    44941 aaaagcagta aaaagggcca gttaactggc ccaaatttta cgagtcattg tttatacatt
    45001 tcgttgatgc gtttgatgca cacggaagct gaagcccagt agctcgcggc tgagcattaa
    45061 agggagccat gcgcctcacg caacattttc tgctctggct gacgaatcgt cgtagaaagc
    45121 gccaatgaca gaatcagcag ggcgaaaatc acgtagaagg tcacatagaa accgccaaac
    45181 agtgaggcga taatcgaacc aaaaatgcta ccgataccga aaccgaggta aatcacaccg
    45241 tagtttttcg ccaggttatt gaggccgaag aactcactga ccagtgatgg aaagacggta
    45301 atagtgccgc caaagttaaa tgccacgcag gcaatcgctg caaagaacgt cactgcattt
    45361 aatggtgcaa acagcagggc cgccataccc accagcgaaa tcacctgacc aatggtgata
    45421 acacggatac gggcaatttt gtcagacaga atgcccagca ccagacgacc tgaaaggtta
    45481 gcgatggaaa taacagtgac tgcattggct gcggaaacca catcaaggtg tgccagactt
    45541 tgggcgatat ctttcgctac cccaatcacg tacaggccgc tcatgcaggc agtcaggaac
    45601 attaccgcta acatccagta ctgcggttta cgcatcgact ctgccagcgt gtaatctttc
    45661 tccaccacac cattgctggt tttcacttcc tgttttggtg cgtctttcat taacgttgcg
    45721 ccgaaaacaa tcatcaccag cgcaatcgct ccccaaatca caaatgtttt ttccaaaccc
    45781 accgtttcga gcagctgcgt gtcgataaat ttgaaaccca ggctacccag accataagaa
    45841 ccgatagcga acgcggagat cagaccttta cgctccggga accacttcac gcagttagag
    45901 agcgtcagca ggtaacccgc gccatccgcc agaccgacca gcacaccggc gcttaaccac
    45961 agcatcatca ggttgttgga atgcgcggtc aggaagaagc ctaatcccag caaaatgccg
    46021 gaagccatgg tgacgcgttt aacgccaaaa cgttcctgca atttgcccgc aacagaagac
    46081 gaaattgcca gccccagact taacaagccg aaagagaaag cgacctggct taccggcgca
    46141 tccagcttgg cggaaagcgc gccattaaac aggctccagg tataaaccga ccccagtgca
    46201 aactgggtaa tgatagtacc gatgagtgtc agccagcggg tacgctgata atttgaaggt
    46261 gtcatggcag tattcctgca gtaataaaaa ggaaaaatct ctgccgacaa ccataacgaa
    46321 gtgcatttta tgcctgggga aaaacggcat gaaatgccat cagaacggaa tgaaatgcct
    46381 tagttcagga atgaatgacg cgacaattat tcaccaaatt tattatttag taagttagca
    46441 cccactatcg ccacaactgt tgttatccaa tggtttcaat tgtgattttt acgttaaaat
    46501 ctggcactaa cccccatcgt atacagatag tcgctattgt tagtggtatt ttctgcctga
    46561 gatagcgcgg cataggcggc aatattttga ttgagcaaca tatcagcgcc tacggtgaca
    46621 tccagccagt tgccgttctg gtttgtcgcc gtcatccggc tcaggcctga ttgcgccttc
    46681 cagatattct cgccaaattg ctggttatag ctgatttgcg cccagggtcg gagatcgcca
    46741 agccgggagt caacacgcca gcctaagcta ctcacgctgg catgccataa cggatcggtc
    46801 agcgtggtag tactgtcgcc aaactcgtta tacatcgagg tggttgtgcc atcgtaatgt
    46861 aatgccgcga ccgggccggt tgtaacctgt tcatataaag gaaaattcca gcccatactc
    46921 atactgctgg tcacatccac aggagtttct tccgccagat tgacttccag tcccagcgcc
    46981 ctttggctac tttgaatacg ttgcgacaaa atatcgttgt aatcaggttg atgatcacta
    47041 tcccaggtgt aacgctctgt ggaatgtccg ggttgcgtat caacgatata ttcttgttgc
    47101 caggcgtaag ctgtgttgag aaaaaaggca ctgattacca cgctcgccag caggcttagc
    47161 tgccagcgac caccgctttt ttttatgatc attaacacga ctccagggaa gagccgaatt
    47221 cggcaatatt attgtcattg tatgaaggat atcgggcata gtagccctgt attaaatatt
    47281 gactttttca ccgatgcgtc aagaaaagcg gctgaaattt ttacgatcgg gtacatagcg
    47341 agggaaagta tggaacgttg cggctgggtg agtcaggacc cgctttatat tgcctaccat
    47401 gataatgagt ggggcgtgcc tgaaactgac agtaaaaaac tgttcgaaat gatctgcctt
    47461 gaagggcagc aggctgggtt atcgtggatc accgtcctca aaaaacgcga aaactatcgc
    47521 gcctgctttc atcagttcga tccggtgaag gtcgcagcaa tgcaggaaga ggatgtcgaa
    47581 agactggtac aggacgccgg gattatccgc catcgaggga aaattcaggc aattattggt
    47641 aatgcgcggg cgtacctgca aatggaacag aacggcgaac cgtttgccga ctttgtctgg
    47701 tcgtttgtaa atcatcagcc acaggtgaca caagccacaa cgttgagcga aattcccaca
    47761 tctacgtccg cctccgacgc cctatctaag gcactgaaaa aacgtggttt taagtttgtc
    47821 ggcaccacaa tctgttactc ctttatgcag gcatgtgggc tggtgaatga tcatgtggtt
    47881 ggctgctgtt gctatccggg aaataaacca tgattcggga agcgcaacgt tcagaactcc
    47941 ccgcgatcct cgaactgtgg ctggaaagta caacctgggg gcatcccttt ataaaagcga
    48001 attactggcg tgactgcatt ccgctggtgc gggatgccta tcttgccaac gcgcaaaact
    48061 gggtctggga agaggacggt aagcttctcg gttttgtcag cattatggaa ggccgatttc
    48121 tggcagcaat gtttgtcgca ccgaaggccg tcagacgcgg tattggtaag gcgctgatgc
    48181 agtatgtgca gcagcgctat ccccatctga tgctggaggt ttatcaaaaa aatcaaccgg
    48241 cgatagattt ttaccgtgcg cagggttttc acattgtcga ttgcgcatgg caggatgaaa
    48301 cccaactacc cacctggatt atgagctggc cggtggttca aacgctgtaa gtgtcagttc
    48361 cggaccgttg tatttttcca gccatgccag cgccgtatta cccgcacagc cattccccag
    48421 ccgcgagctg gggagatctt tggtcagcac gttcactgcg ccgtttttac aaataccgtc
    48481 agcggttaaa tccagatccg gccatgcccc ttcgtgaatg caaatcacgc caggtttaat
    48541 tccctcgcta atgaccgctc cggcaaggat ctgcccacgg gagttccaca cccgcaccat
    48601 atccccttcg gttatgccgc gcgcctgagc atcgtcagga tgaatggtga caggctcacg
    48661 gtttgctacc gcgtacaatt cgcgcagaga actgtaattc agctggctgt gcaggcggtg
    48721 cgccggatgg gcagaaagta cctgcaactg ttctggttcg gcattgccct gccattcgtc
    48781 cggctccagc cacattggat gcccagggca atccgggtaa ccgtaatcgg caatacgctg
    48841 tgagaagatt tcaatcttgc cgctggcggt ttttaacgga tgcgccagcg gatcgcggca
    48901 aaaatcagcg aagcgaataa accgctcgct gtccgggttt tccggcatct caattaactg
    48961 gttggcttgc cagaactcag caaatggcgg caattcaacc tgctggcttg ccccgcgctg
    49021 ccgggcaacg ttataaaacg tttccagcca ttgcagctga ctttttcctt ccgtaaaccg
    49081 cgcatagccg cccttctccc aacgttcact caactcggca aaaacatcaa aatcattacg
    49141 cgcttcatag cgtggcggca ccacttgctt catcggcacc agatgctgat tactgtaatc
    49201 accggtcatg gtgagatcat tacgctcaaa agaggtagtc gcaggcagaa cgatatccgc
    49261 gtgtttcgcc gccgccgtcc agaagcattc agagatcacc accagctccg gtttttgcca
    49321 ggcacggatc agacgattgg tatcctgatg atgagtaaag ttggcaccgc ccgcccacca
    49381 gataaaacga atatccggga aatgtcggtt cataccgttg tgttgatatg cgccaccagg
    49441 gttttccagt gcttcaacaa tgcgggcaac agggatttta tccaccgcat cgcagccacc
    49501 cggcaagctg ccctgcatgg aagagagcac cgcagaacgc cgcgtggggt taccaccatt
    49561 ggcaaaatga taagaaagac caaaaccgcc gccgggtgtg ccgatttgcc ccaacattgc
    49621 cgccagcgtg acgatcatcc agtgtttttg ctcaccaaac tgttggcgct gcattcccca
    49681 gccagccatc agcatggtcg tgttttggtg gaaaagagcc gccagctcgc ggattttcgc
    49741 tgcgttaacc ccacaaatct ctgccgccca ttcggcgttt ttcgctattc cgtcactctc
    49801 gcccagcaaa taagaggcga agacggcata acctgtggtg caacgcgtca gaaacgcttc
    49861 gtcgtgccaa ccattttcca ccagcgtatg ggcgatcccc agcatcagcg caacatcggt
    49921 gcccatgtgc ggtgccaccc actccatttt atcgccaaag aaatcgacgg tttccgatcg
    49981 cattggatca atacagatca gctttttccc gctgtcacgc agtgcggaaa agtaagaaag
    50041 cccctgctca tcagatgcat tccacgcaat tttcagcgta ttgagtgggt tagcactcca
    50101 caacaccacg acatcgctat gttccagcac cagcggccaa ctggtttgct gttgataaac
    50161 ttcgctgcca cccacgacat acggcatgat cgcctgtgcc gcaccggtcg aataatcccc
    50221 cagatgcccg atataaccgc ctgccagcgc catatagcgt tgtaataatg tcgaggcctt
    50281 atgcagcacg ccgtttgaac gccagccgta ggaaccagca aaaatcgatg ccggaccata
    50341 agcctcacga atgcgtttat gttgttggtg aataagatcc agcgcctcat cccaactcac
    50401 gcgaacaaat tcatcctgcc cacgaatgcc ttgcgggttt tccggtgacg cgagaaagcc
    50461 tttgcgcacc attggaaatc gtacccgcgt attgctgtga acctggtcgc gaaccgcgct
    50521 ctgcaaggag ttttccattc ctgtggctaa cgcgccacgc gagctaaaca cggtttcgcc
    50581 gtcggtttca accagcatgg gcccccagtg ggcggcagtc agaacggaat atcgtgagga
    50641 tgagttggcc aaaccgaaga ctcctgaaag gttagatgta ttgagcagtt aaatattact
    50701 tacaaatttc cgagtattcc caggaataat cttcatgttc acgcggcata atctcccgcc
    50761 acggaacccg tggcaaagaa taaaaaggtt attaaggatt aacaatgaag aaacgtgttt
    50821 atcttattgc cgccgtagtg agtggcgctc tggcggtatc tggctgcaca actaaccctt
    50881 acaccggcga acgcgaagca ggcaaatctg ctatcggcgc aggtctgggc tctctcgtgg
    50941 gcgcgggtat tggtgcgctc tcttcttcga agaaagatcg cggtaaaggc gcgctgattg
    51001 gcgcagcagc aggcgcagct ctgggcggcg gcgtgggtta ttacatggat gtgcaggaag
    51061 cgaagctgcg tgacaaaatg cgcggcactg gtgttagcgt aacccgcagc ggggataaca
    51121 ttatcctcaa tatgccgaac aatgtgacct tcgacagcag cagcgcgacc ctgaaaccgg
    51181 caggcgctaa caccctgacc ggcgtggcaa tggtgctgaa agagtatccg aaaacggcgg
    51241 ttaacgtgat tggttatacc gacagcacgg gtggtcacga cctgaacatg cgtctctccc
    51301 agcaacgtgc ggattccgtt gccagcgcgt tgatcaccca gggcgtggac gccagccgca
    51361 tccgtactca gggccttggc ccggctaacc caatcgccag caacagcacc gcagaaggta
    51421 aggcgcaaaa ccgccgtgta gaaattacct taagcccgct gtaatccctt tcatgccagg
    51481 tgatgcagaa atcacctggc attcttcagt tttccttcat catatttcag gctaaggtga
    51541 tcgccttatc agtgaatgga gagaagcatg aagccgtccg ttatcctcta caaagcctta
    51601 cctgatgatt tactgcaacg cctgcaagag catttcaccg ttcaccaggt ggcaaacctc
    51661 agcccacaaa ccgtcgaaca aaatgcagca atttttgccg aagctgaagg tttactgggt
    51721 tcaaacgaga atgtagatgc cgcattgctg gaaaaaatgc cgaaactgcg tgccacatca
    51781 acgatctccg tcggctatga caattttgat gtcgatgcgc ttaccgcccg aaaaattctg
    51841 ctgatgcaca cgccaaccgt attaacagaa accgtcgccg atacgctgat ggcgctggtg
    51901 ttgtctaccg ctcgtcgggt tgtggaggta gcagaacggg taaaagcagg cgaatggacc
    51961 gcgagcatag gcccggactg gtacggcact gacgttcacc ataaaacact gggcattgtc
    52021 gggatgggac ggatcggcat ggcgctggca caacgtgcgc actttggctt caacatgccc
    52081 atcctctata acgcgcgccg ccaccataaa gaagcagaag aacgcttcaa cgcccgctac
    52141 tgcgatttgg atactctgtt acaagagtca gatttcgttt gcctgatcct gccgttaact
    52201 gatgagacgc atcatctgtt tggcgcagaa caattcgcca aaatgaaatc ctccgccatt
    52261 ttcattaatg ccggacgtgg cccggtggtt gatgaaaatg cactgattgc tgcattgcag
    52321 aaaggcgaaa ttcacgccgc cgggctggat gtcttcgaac aagagccact gtccgtagat
    52381 tcgccgttgc tctcaatggc caacgtcgtc gcagtaccgc atattggatc tgccacccat
    52441 gagacgcgtt atggcatggc cgcctgtgcc gtggataatt tgattgatgc gttacaagga
    52501 aaggttgaga agaactgtgt gaatccgcac gtcgcggact aagccgcgac tgcgtgaagt
    52561 aaagcccgat aatcgctcgg gcttttactc tttattgggt tgcagtaact gctgtagtcc
    52621 aggcctgatt aaacgcctga tgttgtgccg gtaacggcgc aatcagtttg ttatattcac
    52681 ttgcctgctg cgaagtcggg aactggatac catttgctac aaagctcacc tgagtacctt
    52741 gttgagcaat gtagtctcca accatcacca gttgttgcgt gaaagtttgt gcagccggaa
    52801 tcaacggttg caatgcatca gctggcgttg ttaccacttt ggtgaatgcc tgatcaaaga
    52861 ccggtttcaa atcatcggtc tgttttagcg cagagtgcgc agcatcggcc tgcaacttcg
    52921 cattttgcag ttgttgcgcc agcacaccca gcgaaccgtt catttcacgc agcggaccgc
    52981 tttgcgtaac gtaatcctgc ggtacgcgaa ttgcgttaac gctgtctacg accgggcgca
    53041 gaccggaatc catcgcctga ttcacctgct gagaataacc atacagaatc gcgtaatcag
    53101 agacaaaagg accaaactgt tttttctgat cggcagtcag ggttggtaga cgttcaccgc
    53161 tacgcatcac tgtattctgc aggaagtcga taaacgcttt acgctgatcg ccttctttat
    53221 caaaacaccc actcaggcta actaccatta ataacgccgc aagaggcgca aaccagcgag
    53281 agcaggactt tcctgtcgcc attttcttac tcctttcacc caaaaaaagc gcacaacgac
    53341 acacgcgtgt ctgacgtgac aaggatagtc caggacaggc ttgcaggata ccctttctac
    53401 gtaatcttcc tggtaaaatc atggagaaaa tgaaacaagg ttaatttgtt gtttcattgt
    53461 taaaaaatga tagtgccgtt gccttttttg tagccagaga tttacgcctt cgcagaaggt
    53521 tagattttcg aaatctttac aaaacaggag tggtaagaga tatatcggca tttatcagat
    53581 cgttaggtgg ctataagtca cgctaaatga caggctgaat cgaatcatag ccagagcatg
    53641 ccctgacttc accccgctgt gtctgctttt cccgactatt cttaatgagc ttcgatgcaa
    53701 ttcacgatcc cgcagtgtga tttgaggagt tttcaatgga atataaagat ccaatgcatg
    53761 agctgttgag cagcctggaa cagattgttt ttaaagatga aacgcagaaa attaccctga
    53821 cgcacagaac aacgtcctgt accgaaattg agcagttacg aaaagggaca gggttaaaaa
    53881 tcgatgattt cgcccgggtt ttgggcgtat cagtcgccat ggtaaaggaa tgggaatcca
    53941 gacgcgtgaa gccttcaagt gccgaactaa aattgatgcg tttgattcaa gccaacccgg
    54001 cattaagtaa gcagttgatg gaatagactt ttatccactt tattgctgtt tacggtcctg
    54061 atgacaggac cgttttccaa ccgattaatc ataaatatga aaaataattg ttgcatcacc
    54121 cgccaatgcg tggcttaatg cacatcaacg gtttgacgta cagaccatta aagcagtgta
    54181 gtaaggcaag tcccttcaag agttatcgtt gatacccctc gtagtgcaca ttcctttaac
    54241 gcttcaaaat ctgtaaagca cgccatatcg ccgaaaggca cacttaatta ttaaaggtaa
    54301 tacactatgt ccggtaaaat gactggtatc gtaaaatggt tcaacgctga caaaggcttc
    54361 ggcttcatca ctcctgacga tggctctaaa gacgtgttcg tacacttctc tgctatccag
    54421 aacgatggtt acaaatctct ggacgaaggt cagaaagtgt ccttcaccat cgaaagcggc
    54481 gctaaaggcc cggcagctgg taacgtaacc agcctgtaat ctctgcttaa aagcacagaa
    54541 tctaagatcc ctgccatttg gcggggattt ttttatttgt ttttcaggaa ataaataatc
    54601 gatcgcgtaa taaaatctag tattattttt gtgaaaaata aatttgggtg caatgagaat
    54661 gcgcaacgcc gtaagaaagg cgggaataac ttcccgccga agactcttac tcttttaaat
    54721 tgcaggctaa aaatgccgcc agctcataac tcccctgttt aatatgcaat tcacacagtg
    54781 aatctcttat catccaggtg aaaaataaaa gcgtgaaaca aatcactatt aaagaaagta
    54841 atctatattt ctgcggcatt cccagccccc tgtgttgatt tccaacgagt aagaggctaa
    54901 ccttatggtg ttcaggcata gggtagcctc actttgattt atagtcaggt ggggcttttc
    54961 tctgtctgcc tttcggtgaa tacctgagac aaacagtctc aagcacccgt ggctattcta
    55021 gcttaataag
//
LOCUS       DAMZII010000017        54398 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZII010000017 DAMZII010000000
VERSION     DAMZII010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.017"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            5..232
                     /locus_tag="POA77_000969"
     CDS             5..232
                     /locus_tag="POA77_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDI6364441.1"
                     /translation="MLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLLRGW
                     IYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            235..1092
                     /locus_tag="POA77_000970"
     CDS             235..1092
                     /locus_tag="POA77_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6364442.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1092..3104
                     /locus_tag="POA77_000971"
     CDS             1092..3104
                     /locus_tag="POA77_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6364443.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3105..3626)
                     /gene="sodC"
                     /locus_tag="POA77_000972"
     CDS             complement(3105..3626)
                     /gene="sodC"
                     /locus_tag="POA77_000972"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDI6364444.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3707..4603)
                     /locus_tag="POA77_000973"
     CDS             complement(3707..4603)
                     /locus_tag="POA77_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDI6364445.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4652..4891)
                     /locus_tag="POA77_000974"
     CDS             complement(4652..4891)
                     /locus_tag="POA77_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDI6364446.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4994..5593
                     /gene="nemR"
                     /locus_tag="POA77_000975"
     CDS             4994..5593
                     /gene="nemR"
                     /locus_tag="POA77_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDI6364447.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFVPLENALAHVKNIIATPAV"
     gene            5630..6727
                     /gene="nemA"
                     /locus_tag="POA77_000976"
     CDS             5630..6727
                     /gene="nemA"
                     /locus_tag="POA77_000976"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDI6364448.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANADLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6808..7215
                     /gene="gloA"
                     /locus_tag="POA77_000977"
     CDS             6808..7215
                     /gene="gloA"
                     /locus_tag="POA77_000977"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDI6364449.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7318..7965
                     /gene="rnt"
                     /locus_tag="POA77_000978"
     CDS             7318..7965
                     /gene="rnt"
                     /locus_tag="POA77_000978"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDI6364450.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8058..12674
                     /locus_tag="POA77_000979"
     CDS             8058..12674
                     /locus_tag="POA77_000979"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6364451.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELSEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12725..13072)
                     /gene="grxD"
                     /locus_tag="POA77_000980"
     CDS             complement(12725..13072)
                     /gene="grxD"
                     /locus_tag="POA77_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDI6364452.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMNQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13406..14221
                     /gene="mepH"
                     /locus_tag="POA77_000981"
     CDS             13406..14221
                     /gene="mepH"
                     /locus_tag="POA77_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDI6364453.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLVDAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14349..14930
                     /gene="sodB"
                     /locus_tag="POA77_000982"
     CDS             14349..14930
                     /gene="sodB"
                     /locus_tag="POA77_000982"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDI6364454.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15076..16245)
                     /locus_tag="POA77_000983"
     CDS             complement(15076..16245)
                     /locus_tag="POA77_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364455.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16411..16500)
                     /gene="ynhF"
                     /locus_tag="POA77_000984"
     CDS             complement(16411..16500)
                     /gene="ynhF"
                     /locus_tag="POA77_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDI6364456.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16799..17824
                     /gene="purR"
                     /locus_tag="POA77_000985"
     CDS             16799..17824
                     /gene="purR"
                     /locus_tag="POA77_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDI6364457.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17821..18753)
                     /locus_tag="POA77_000986"
     CDS             complement(17821..18753)
                     /locus_tag="POA77_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6364458.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18866..20077
                     /locus_tag="POA77_000987"
     CDS             18866..20077
                     /locus_tag="POA77_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDI6364459.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20368..21516
                     /gene="cfa"
                     /locus_tag="POA77_000988"
     CDS             20368..21516
                     /gene="cfa"
                     /locus_tag="POA77_000988"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDI6364460.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21556..22197)
                     /gene="ribE"
                     /locus_tag="POA77_000989"
     CDS             complement(21556..22197)
                     /gene="ribE"
                     /locus_tag="POA77_000989"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI6364461.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22412..23785
                     /gene="mdtK"
                     /locus_tag="POA77_000990"
     CDS             22412..23785
                     /gene="mdtK"
                     /locus_tag="POA77_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDI6364462.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(23826..25082)
                     /locus_tag="POA77_000991"
     CDS             complement(23826..25082)
                     /locus_tag="POA77_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDI6364463.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25390..25466
                     /locus_tag="POA77_000992"
     tRNA            25390..25466
                     /locus_tag="POA77_000992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25424..25426,aa:Val,seq:gac)
     gene            25471..25547
                     /locus_tag="POA77_000993"
     tRNA            25471..25547
                     /locus_tag="POA77_000993"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25505..25507,aa:Val,seq:gac)
     gene            25655..25960
                     /locus_tag="POA77_000994"
     CDS             25655..25960
                     /locus_tag="POA77_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDI6364464.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26086..27690
                     /locus_tag="POA77_000995"
     CDS             26086..27690
                     /locus_tag="POA77_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDI6364465.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27702..28514)
                     /locus_tag="POA77_000996"
     CDS             complement(27702..28514)
                     /locus_tag="POA77_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364466.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28518..29303)
                     /locus_tag="POA77_000997"
     CDS             complement(28518..29303)
                     /locus_tag="POA77_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDI6364467.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVWGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29300..29968)
                     /locus_tag="POA77_000998"
     CDS             complement(29300..29968)
                     /locus_tag="POA77_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI6364468.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30032..30670)
                     /locus_tag="POA77_000999"
     CDS             complement(30032..30670)
                     /locus_tag="POA77_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDI6364469.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30683..32785)
                     /locus_tag="POA77_001000"
     CDS             complement(30683..32785)
                     /locus_tag="POA77_001000"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDI6364470.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(32806..33432)
                     /locus_tag="POA77_001001"
     CDS             complement(32806..33432)
                     /locus_tag="POA77_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDI6364471.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKSCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(33888..34097)
                     /gene="fumD"
                     /locus_tag="POA77_001002"
     CDS             complement(33888..34097)
                     /gene="fumD"
                     /locus_tag="POA77_001002"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDI6364472.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            34654..36066
                     /gene="pykF"
                     /locus_tag="POA77_001003"
     CDS             34654..36066
                     /gene="pykF"
                     /locus_tag="POA77_001003"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDI6364473.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            36377..36613
                     /gene="lpp"
                     /locus_tag="POA77_001004"
     CDS             36377..36613
                     /gene="lpp"
                     /locus_tag="POA77_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDI6364474.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(36677..37681)
                     /gene="ldtE"
                     /locus_tag="POA77_001005"
     CDS             complement(36677..37681)
                     /gene="ldtE"
                     /locus_tag="POA77_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDI6364475.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(37830..38246)
                     /gene="sufE"
                     /locus_tag="POA77_001006"
     CDS             complement(37830..38246)
                     /gene="sufE"
                     /locus_tag="POA77_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDI6364476.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(38259..39479)
                     /gene="sufS"
                     /locus_tag="POA77_001007"
     CDS             complement(38259..39479)
                     /gene="sufS"
                     /locus_tag="POA77_001007"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDI6364477.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(39476..40747)
                     /gene="sufD"
                     /locus_tag="POA77_001008"
     CDS             complement(39476..40747)
                     /gene="sufD"
                     /locus_tag="POA77_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI6364478.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(40722..41468)
                     /gene="sufC"
                     /locus_tag="POA77_001009"
     CDS             complement(40722..41468)
                     /gene="sufC"
                     /locus_tag="POA77_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI6364479.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(41478..42965)
                     /gene="sufB"
                     /locus_tag="POA77_001010"
     CDS             complement(41478..42965)
                     /gene="sufB"
                     /locus_tag="POA77_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI6364480.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(42974..43342)
                     /gene="sufA"
                     /locus_tag="POA77_001011"
     CDS             complement(42974..43342)
                     /gene="sufA"
                     /locus_tag="POA77_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDI6364481.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(43890..44078)
                     /locus_tag="POA77_001012"
     CDS             complement(43890..44078)
                     /locus_tag="POA77_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDI6364482.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(44178..44588)
                     /gene="menI"
                     /locus_tag="POA77_001013"
     CDS             complement(44178..44588)
                     /gene="menI"
                     /locus_tag="POA77_001013"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDI6364483.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(44585..47641)
                     /locus_tag="POA77_001014"
     CDS             complement(44585..47641)
                     /locus_tag="POA77_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI6364484.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            48030..49142
                     /gene="ydiK"
                     /locus_tag="POA77_001015"
     CDS             48030..49142
                     /gene="ydiK"
                     /locus_tag="POA77_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDI6364485.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            49328..49435
                     /gene="rprA"
                     /locus_tag="POA77_001016"
     ncRNA           49328..49435
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="POA77_001016"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            49571..49927
                     /locus_tag="POA77_001017"
     CDS             49571..49927
                     /locus_tag="POA77_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDI6364486.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            50027..51241
                     /locus_tag="POA77_001018"
     CDS             50027..51241
                     /locus_tag="POA77_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364487.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVADMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            51468..52733
                     /locus_tag="POA77_001019"
     CDS             51468..52733
                     /locus_tag="POA77_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364488.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            52745..53611
                     /gene="ydiB"
                     /locus_tag="POA77_001020"
     CDS             52745..53611
                     /gene="ydiB"
                     /locus_tag="POA77_001020"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDI6364489.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            53642..>54398
                     /gene="aroD"
                     /locus_tag="POA77_001021"
     CDS             53642..>54398
                     /gene="aroD"
                     /locus_tag="POA77_001021"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI6364490.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT        13141 a        14162 c        13230 g        13865 t
ORIGIN      
        1 gtttatgctc aatgcaacag gattgccctt acaagatctg gtgttcggtg cgtccgtcta
       61 ttttcctccg tttttcaaag ccttcgcgtt tggattcgtc atctggcttg tcgtacaccg
      121 cctgcttcgt ggctggatct acgccggtga catctggcat cccttgttaa tggatttatc
      181 gctgtttgcg atttgcgttt gccttgctct ggcaatactg attgcgtggt aactatgtca
      241 attaaaacaa ttaagtattt ctcaacaatc attgtagcga tagttgcggt tcttgccgga
      301 tggtggctgt ggaattatta catgcaatct ccgtggacac gcgacgggaa aatacgcgca
      361 gaaaaggttt ctatcacccc acaggtgtcg ggacgtattg ttgagctgaa tataaaagac
      421 aaccaactgg ttaacgcggg ggatcttctg ctcacgatcg ataaaacgcc ctttcagatc
      481 gccgagctga acgcacaggc tcagctggcg aaagcgcaat ctgacctcgc aaaagccaat
      541 aacgaagcga atcgtcgtcg tcatctctca caaaatttta tttctgccga agaactcgat
      601 accgctaacc tcaatgttaa agcgatgcag gccagtgtta atgccgcaca ggccacgctg
      661 aaacagacac aatggcaact ggcgcaaacg gaaattcgcg ccccggtgag tggttgggtg
      721 actaacctca ctacccgcat cggcgattac gctgacaccg ggaaaccgct gtttgccctt
      781 gtcgacagcc actcgtttta cgtcattggt tattttgaag aaaccaagtt gcgccatatc
      841 cgcgaaggtg caccagcaca aattaccctc tatagtgata acaaaacgtt acggggtcac
      901 gtttccagta tcggtcgggc gatttatgat caaagcgttg aaagtgactc aagccttatt
      961 ccggatgtga aacctaacgt cccctgggtt cgtctcgccc aacgcgttcc cgtccgcttt
     1021 gcgctggata aagttccagg cgatgtcacg ctggtgtcgg gcaccacctg tagcattgcc
     1081 gtaggtcaat aatgaacgca tcgtcatggt ccttgcgcaa tttgccctgg ttcaaggcca
     1141 cgctggcgca atggcgttat gcgttacgca ataccattgc catgtgtctg gcgctgacgg
     1201 ttgcctatta tttgaatctg gatgaaccct attgggcgat gacctcggct gcagtggtta
     1261 gctttcccac cgttggcggt gtcatcagca aaagcctcgg acgcatcgct ggcagtttac
     1321 tgggagccat tgcggcactg cttcttgccg ggcatacgct caatgagccg tggttttttc
     1381 tattgagcat gtcggcgtgg cttggctttt gtacctgggc ctgtgcgcac ttcacgaata
     1441 acgtcgcgta tgcatttcaa ctggcgggct acacggctgc catcatcgcc tttccgatgg
     1501 ttaatattac tgaggccagc cagctgtggg atatcgctca ggcgcgcgtt tgcgaggtga
     1561 tagtcggtat tttgtgcggc ggcatgatga tgatgatcct gcctagcagt tccgatgcta
     1621 cagccctttt aaccgcattg aaaaacatgc acgcccgatt actggaacat gccagtttac
     1681 tctggcagcc tgaaacaaac gatgccattc gtgcagcaca tgagggggtg attgggcaga
     1741 tactgaccat gaatttgctg cgtatccagg ctttctggag ccactatcgt tttcgccagc
     1801 aaaacgcgcg ccttaatgcg ctactccacc agcaattacg tatgaccagt gtcatctcca
     1861 gcctgcgacg tatgttgctc aactggccct caccgccagg tgccacgcga gaaattctcg
     1921 aacaattgct gacggcgctc gccagttcgc aaacagatgc ttacaccgtc gcacgtatta
     1981 tcgccccgct acgcccgacc aacgtcgccg actaccggca cgtcgccttc tggcagcgac
     2041 tacgttattt ttgccgcctt tatctgcaaa gtagtcagga attacatcgt ctgcaaagcg
     2101 gtgtagatga tcatgccaga ctcgcacgga catccgctct ggctcgtcat accgacaacg
     2161 ccgaagctat gtggagcggg ctgcgtacat tttgtacgtt gatgatgatt ggcgcatgga
     2221 gtattgcatc gcaatgggat gccggtgcca atgcattaac gctggcagca attagctgcg
     2281 tactctactc cgccgtcgca gcaccgttta agtcgttgtc actgctgatg cgcacgctgg
     2341 tgttactttc gctattcagc tttgtggtca aatttggcct gatggtccag attagcgatc
     2401 tgtggcaatt tttactgttt ctctttccac tgctggcgac aatgcagctt cttaaattgc
     2461 agatgcccaa atttgccgca ctgtgggggc aactgattgt ttttatgggt tcgtttatcg
     2521 ctgtcactaa tcccccggtg tatgattttg ctgattttct taacgataat ctggcaaaaa
     2581 tcgttggcgt tgcgctggct tggctagcgt tcgccattct gcgtccagga tcggatgctc
     2641 gtaaaagccg ccgccatatt cgcgcgctgc gccgggattt tgtcgatcag ctaagccgcc
     2701 atccaacact gagtgaaagc gaatttgaat cgctcactta tcatcacgtc agtcagttga
     2761 gtaacagcca ggatgcgctg gctcgccgtt ggttattacg ctggggtgta gtgctgctga
     2821 actgttctca tgttgtctgg caattgcgcg actgggaatc gcgttccgat ccgttatcgc
     2881 gagtacggga taactgtatt tcactgttgc ggggagtgat gagtgagcgt ggcgttcagc
     2941 aaaaatcact ggcggccaca cttgaagaat tacagcggat ttgcgacagc cttgcccgtc
     3001 atcatcaacc tgccgcccgt gagctggcgg caattgtctg gcggctgtac tgctcgcttt
     3061 cgcaacttga gcaagcacca ccgcaaggta cgctggcctc ttaattactt aattacacca
     3121 caggcatagc gttcaccgcc accgcccagc ggtttaggtt gatcggacat attatcgccg
     3181 ccaacgtgga ccatcagcgc tttgtctttg atttcatcca gtgatttcag acgaggcgcg
     3241 atgacggcat cggtagcttt gccgtcatta ttgacgacca gtgcaggcag atcgcctaaa
     3301 tgcccggcac cttctggccc ttcatgttta ccggtatttt gtggatcaag atgcccgcct
     3361 gcggattccg cggcgctggc tttgccatct ttggtggctg gctggcagct tcctttggca
     3421 tgaatatgga agccatgttc accggggggt aatgctttca gatcgggcga aaactccaga
     3481 cctttatcgg tttcagtaat ggtgacgcta ccaattgact gccctacccc ttgcgacgtg
     3541 acgaggttca tctcgacttt ttcactggca gcttgtgcgc cggttgcaac aaccagcgcc
     3601 agaatagcca gactaaaacg tttcatagga cctccgttca ttgtgcattc ctgaataagt
     3661 gtaaaccagt gacacatttt tgaacggcgg ctattcctaa aagtgcttac ggtacgtcgt
     3721 accccagtgc cgctttacgg atacgaaacc attgttgacg ggtcattttc agtgtttctg
     3781 cttcgacagc tgcccgtacg cgctcaattt tacctgaacc gataattggc agcggctgcg
     3841 atggtaaacg taatacccag gcgtaaacca cctgttcaat cgagcccgcg tttaactcct
     3901 ctgccaccac agccagttca tcacgcagct gctggaaata atcatcatta aacagacgac
     3961 caccaccaag gcaggaccac gccatcggac gaacacgcag ttgttgtagt tggtcgagcg
     4021 tgccatccag cagtaacggc tgatgcaccg gggatatttc cacctgatta gtggcaaggg
     4081 taaacggcag acgtgattgc aacagggcaa attgcgcagg cgtaaagttc gatacgccaa
     4141 aatgacgcac tttgccgctc tgatgcagat gtttgaacgc gtccgccact tcatcggcat
     4201 ccattaacgg gtctggtcgg tggattaaca gcagatccag atgatcggtc gcgagattaa
     4261 ttagcgactg ttcggcgctc ttaatgatgt gatcgcggtc agtgatgtaa tgaccaatga
     4321 cgttttcttc acgcgcggtc gtcgcgatac cgcatttact gacgatttcc atccgttcac
     4381 gcaggtgagg tgccagtttc agtgcctcgc caaacgccgc ttcgcactga tagccaccat
     4441 aaatatcagc atggtccacg gtggtcacgc cgagatccag atgctcttca ataaaactga
     4501 ccagctggcg ggcggacata ttccagtcca tcaatcgcca gtagcccatc acaaaacggg
     4561 aaaactccgg gccttgcggc gcaatagtaa tacgctgaac cataatcact tcctcttatc
     4621 agatatgaga ggagtatacg caagattagg ttcaaaagag tgatggttgc tccggttcgt
     4681 ctgatgacgc tggcttattt gcgcgtaatt tgcgcattaa tcgctgccga caaaggcgca
     4741 gcacctcttg tttttcgcca tcgctcattt tattccagtt aaaacgctca tcacgactac
     4801 gaaaacagcc gcgacaaaac ccgcgttcgt cagactggca aattccccgg cacgggctct
     4861 ggacgggaaa gaactctaat tgctccgcca cttcgccctc ctcagataag attattacca
     4921 ttattgaagc tgttaatgtc caaagtagca actttgcttg cactagaccg actggtctac
     4981 tacactccaa cgcatgaaca aacacaccga acatgatact cgcgaacatc tcctggcgac
     5041 gggcgagcaa ctttgcctgc aacgtggatt caccgggatg gggctaagcg aattactaaa
     5101 aaccgctgaa gtgccgaaag ggtccttcta tcactacttt cgctctaaag aagcgtttgg
     5161 cgttgccatg cttgagcgtc attacgccgc atatcaccag cgactgactg agttgctgca
     5221 atccggcgaa ggtaactacc gcgaccgcat actggcttat taccagcaaa cactgaacca
     5281 gttttgccaa catggaacca tcagtggttg cctgacagta aaactctctg ccgaagtgtg
     5341 cgatctgtca gaagatatgc gcagcgcgat ggataaaggt gctcgcggcg tgatcgccct
     5401 gctctctcag gcgctggaaa atggccgtga gaaccattgt ttaacctttt gtggcgaacc
     5461 gctacaacag gcacaagtgc tttacgcact gtggcttggc gcgaatctgc aggccaaaat
     5521 ttcgcgcagt ttcgtgccac tggaaaacgc gctggcccat gtaaaaaaca ttattgcgac
     5581 gcctgccgtt tagcaggcat tttttatcac cagacgaccg ggagccttta tgtcatctga
     5641 aaaactgtat tccccactga aagtgggcgc gatcacggcg gcaaaccgta tttttatggc
     5701 accgctgacg cgtctgcgca gtattgaacc gggtgacatt cctaccccgt tgatggcgga
     5761 atactatcgc caacgtgcca gtgccggttt gattattagt gaagccacgc aaatttctgc
     5821 ccaggcaaaa ggatatgcag gtgcgcctgg catccatagt ccggagcaaa ttgccgcatg
     5881 gaaaaaaatc accgctggcg ttcatgctga aaatggtcat atggccgtgc agctgtggca
     5941 caccggacgc atttctcacg ccagcctgca acctggcggt caggcaccgg tagcgccttc
     6001 agcacttagc gcgggaacac gtacttctct gcgcgatgaa aatggtcagg cgatccgtgt
     6061 tgaaacatcc atgccgcgtg cgcttgaact ggaagagatt ccaggtatcg tcaatgattt
     6121 ccgtcaggcc attgctaacg cgcgtgaagc cggttttgat ctggtagagc tccactctgc
     6181 tcacggttat ttgctgcatc agttcctttc tccttcttca aaccatcgta ccgatcagta
     6241 cggcggcagc gtggaaaatc gcgcacgttt ggtactggaa gtggtcgatg ccgggattga
     6301 agaatggggt gccgatcgca ttggcattcg cgtttcgcca atcggtactt tccagaacac
     6361 agataacggc ccgaatgaag aagccgatgc actgtatctg attgaacaac tgggtaaacg
     6421 cggcattgct tatctgcata tgtcagaacc agattgggcg gggggtgaac cgtatactga
     6481 tgcgttccgc gaaaaagtac gcgcccgttt ccacggtccg attatcggcg caggtgcata
     6541 cacagtagaa aaagctgaaa cgctgatcgg caaagggtta attgatgcgg tggcatttgg
     6601 tcgtgactgg attgcgaacg cggatctggt cgcccgcttg cagcgcaaag ctgagcttaa
     6661 cccacagcgt gccgaaagtt tctacggtgg cggcgcggaa ggctataccg attacccgac
     6721 gttgtaatcc aacattgcga gcggcgtaaa gccgccgcta tactaaaaca acattttgaa
     6781 tctgttagcc attttgagga taaaaagatg cgtcttcttc ataccatgct gcgcgttggc
     6841 gatttgcaac gctccatcga tttttatacc aaagtgctgg gcatgaaact gctgcgtacc
     6901 agcgaaaacc cggaatacaa atactcactg gcgtttgttg gctacggccc ggaaaccgaa
     6961 gaagcggtga ttgaactgac ctacaactgg ggcgtggata aatacgaact cggcactgct
     7021 tatggtcaca tcgcgcttag cgtagataac gccgctgaag cgtgcgaaaa aatccgtcaa
     7081 aacgggggta acgtgacccg tgaagcgggt ccggtaaaag gcggtactac ggttatcgcg
     7141 tttgtggaag atccggacgg ttacaaaatt gagttaatcg aagaaaaaga cgccggtcgc
     7201 ggtctgggca actaatctcc tgccgggcgt gaactcatcg cgcccgcatc tttactgcat
     7261 cgacaagtaa tatttgtcat aatgcgcgct gcaatttatc cgtattaaga gaatcagatg
     7321 tccgataacg ctcaacttac cggtctgtgc gaccgttttc gtggttttta tcctgttgtg
     7381 atcgatgttg aaacagccgg atttaacgcc aaaaccgatg cgctgcttga gattgccgcc
     7441 atcaccctga aaatggatga acaaggctgg ctgatgccgg acaccacatt acatttccac
     7501 gtcgaaccat ttgtcggcgc aaatttgcag ccagaggccc tcgccttcaa cggcattgac
     7561 ccgaacgatc ccgatcgcgg cgcggtcagc gaatacgagg cgctgcacga aatttttaaa
     7621 gttgtacgta aaggtattaa agcgagcggc tgtaaccgcg ccattatggt ggcgcacaat
     7681 gccaattttg atcacagctt tatgatggcc gccgcagaac gcgcctcact gaaacgtaac
     7741 ccgttccacc ctttcgccac ttttgacacc gctgcactgg ccgggctggc actcggacaa
     7801 accgtattgt caaaggcttg ccagaccgct ggcatggact tcgacagcac ccaggcgcac
     7861 tccgcgctgt acgacaccga acgcactgct gtgctgtttt gtgaaatcgt caaccgctgg
     7921 aaacgtctgg gaggctggcc gctacctgcc gccgaagagg tgtaatcgag tcgatgcctg
     7981 atgacatgca atgattcagg catctatagt gaggctattc cacgcatcct gcatgatatt
     8041 cacggggaat agcgttaatg gcagataatc cagacccttc atcgctcctg ccggacgtgt
     8101 tttcaccggc gacccgcgac tggtttcttc gcgcctttaa acagccgacc gctgtccagt
     8161 cgcaaacctg gcatgtggcg gcgcgaagcg aacatgcgct ggtgattgca ccgaccggct
     8221 ccgggaaaac gctggcagca tttctctacg ccctcgatcg gctcttccgc gaaggcggcg
     8281 aagatacccg cgaggcgcat aagcgtaaaa cctcacgcat cctctatatt tcaccgataa
     8341 aagctctggg caccgacgtt cagcgcaact tgcagctccc gttgaagggt attgccgatg
     8401 aacggcggcg gcgcggcgaa acggaagtca atcttcgcgt agggatccgt actggcgata
     8461 cgcctgcaca ggaacgcagc aaactcaccc gtaatccgcc ggatattctg atcaccacac
     8521 ccgaatcact ctatctgatg ctgacctccc gcgcgcgcga aacgctacgc ggcgtcgaaa
     8581 cggtaattat tgatgaagtc cacgcggtag cgggcagtaa acgtggtgcg catctggcgt
     8641 taagtctgga gcggctcgat gcgctgctcc acacctcagc acagcgaatt ggcctttctg
     8701 ccactgtgcg ctcagccagc gatgtggcag catttcttgg tggcgatcgc ccggttacgg
     8761 tagtcaaccc gcccgcaatg cgccatccgc agatacgaat tgtcgtaccg gtcgccaata
     8821 tggatgatgt ctcatcggtc gccagcagca ccggcgaaga cagccatgcc ggccgggaag
     8881 gctccatctg gccatatatt gaaacgggta tccttgatga agtgttgcgc catcgctcga
     8941 ccattgtctt tactaattca cgtgggctgg cggaaaaact gacggcacga ttaaatgagc
     9001 tttacgccgc acgcttacag cgttccccgt ctatcgccgt tgatgcggcc catttcgagt
     9061 cgacctccgg cgcaacctct aaccatgtac aaagtagcga cgtttttatt gcccgctcac
     9121 accacggctc cgtctctaaa gaacaacgag caatcaccga acaggcgctg aaatcgggtg
     9181 aattacgctg cgtggtcgca acctccagtc tcgaactggg gattgatatg ggcgcggtgg
     9241 atctggtgat tcaggtggca acgccgcttt ctgttgccag tgggttacaa cgcattggtc
     9301 gcgccggaca tcaggttggc ggtgtatcta aagggctgtt tttccctcgt acccggcgtg
     9361 acttagtcga ttccgcagtc attgtagagt gtatgttcgc aggcaggctg gaaaacctga
     9421 caccgccgca taatccactc gacgtccttg cgcagcaaac cgttgccgcc gcggcgatgg
     9481 atgcattaca ggtagacgaa tggtactccc gcgtacgccg tgccgcaccg tggaaagatc
     9541 tgccaagacg tgtttttgac gccacgctgg atatgctttc cgggcgctat ccctctggcg
     9601 atttttctgc ttttcgcccc aaactggtct ggaacaggga gaccgggata ttgaccgccc
     9661 gacctggcgc tcaattgttg gcggttacca gcggcggcac cattccggat cgtggcatgt
     9721 atagcgtgtt attacccgaa ggtgaagaac aggccggttc gcggcgggtg ggtgaactgg
     9781 atgaggagat ggtatatgag tcgcgggtga acgacattat cactctcggc gctacctcat
     9841 ggcggatcca gcaaatcacc cgcgatcagg tgattgtgac tcctgctccg ggtcgttctg
     9901 cccggctccc cttctggcgt ggtgaaggta acggacgtcc ggctgaatta agcgagatga
     9961 tcggcgattt tcttcattta ctggcggatg gcgcgttctt ttccgggact attcccccgt
    10021 ggctggcaga agaaaatacg aacgccaata ttcaggggtt gattgacgag caacgcaacg
    10081 cgacgggcat cgttccgggg agtcgccatc tggtcctcga acggtgccgt gatgaaattg
    10141 gcgactggcg tattattttg cactctccct atggaagacg ggtgcatgaa ccctgggcgc
    10201 tggcgattgc cgggcgaata catgccctat ggggcgctga cgcgtctgtg gtcgccagtg
    10261 atgacggcat tgttgcgcgc attcctgaca ctgatggtaa attgcccgac gccgcgattt
    10321 ttttgtttga accagaaaag ttgctgcaaa ttgtccgcga ggcggtaggc agctcggcac
    10381 ttttcgccgc ccgttttcgc gaatgcgccg cgcgggcatt attaatgccg gggcgcactc
    10441 cgggccatcg caccccgctt tggcaacaac ggctgcgcgc cagtcagttg ctggaaatcg
    10501 ctcagggata tccggatttt ccggtcattc tcgaaaccct acgcgaatgt ctgcaagatg
    10561 tttatgatct tcccgcactg gaacgtttga tgcgtcgcct gaacggtggc gaaattcaaa
    10621 tatccgatgt aacgactact acgccctcgc ctttcgccac cagtttattg ttcggctatg
    10681 tcgcggaatt tatgtaccag agcgacgccc cgctggcaga gcgccgggca tccgtactgt
    10741 cgctggacag cgagttactg cgcaatctac tcggacaggt cgatccgggg gaattactcg
    10801 acccgcaggt cattcgccag gtggaagaag agttgcaacg actggctcct ggcagaagag
    10861 cgaaaggtga agaaggattg ttcgacctgc tgcgcgaact ggggccaatg accgttgaag
    10921 acctggcgca acggcataca ggcagcagtg aagaggttgc gtcgtatctg gaaaatcttc
    10981 ttgcagtaaa acgaatcttc ccagcgatga ttagcggaca ggagcgtctt gcctgtatgg
    11041 atgatgccgc caggctgcgt gatgccctcg gcgtacgact accagagtca ttgccagaga
    11101 tttatttaca tagagtcagt tacccgcttc gcgatctctt tctgcgctat ctccgggctc
    11161 atgctctggt cacggctgaa caactggctc atgagtttag tctcggtatt gccattgtcg
    11221 aagagcagct tcagcaactg cgtgaacagg gtctggtgat gaatctgcaa caagacatct
    11281 gggtgagcga tgaagtattt cgtcgtctgc gtttgcgctc gctgcaagcc gccagagaag
    11341 cgacgcgtcc cgttgcagcc acgacctatg cgcgattgct gctggaacgt cagggcgtat
    11401 tacccgccac cgatggtagc ccggcgctct ttgcctcaac atcgccaggc gtttatgagg
    11461 gcgtagatgg cgtgatgcgg gtgatcgaac agcttgccgg agtcggttta cccgcctcac
    11521 tctgggaaag ccagatcctg cctgcccgcg tacgcgacta ttcgtcagaa atgctcgatg
    11581 aattactggc aaccggtgcg gttatctggt cggggcaaaa aaaactgggt gaagatgacg
    11641 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    11701 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    11761 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    11821 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    11881 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    11941 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgcgggtat
    12001 cttacaacac accaaatctg gctggacgct ggtcgttatt gcaggtggag ccactaaacg
    12061 ataccgaaag gatgctggcg ctggcggaaa atatgctcga ccgctacggc atcatcagtc
    12121 gtcaggcggt gatagccgaa aatatccctg gcgggtttcc atcgatgcaa acgctttgtc
    12181 gaagtatgga agactccggg cgaattatgc gaggtcgttt tgtagaaggt ctgggtggcg
    12241 cgcaattcgc tgaacgtctg actattgacc gattgcgcga tctggcgaca caagccacgc
    12301 aaacgcgcca ctatacacca gtggcgctct ctgccaacga tccggctaat gtgtggggaa
    12361 atcttctgcc ctggcctgca catccggcaa cgctggttcc aacgcgtcgg gcgggtgcgc
    12421 tggtggtcgt ttctggcggc aaattgttac tctatctggc gcaaggtggc aaaaaaatgc
    12481 tggtctggca ggaaaaagag gaattactcg ccccagaggt tttccacgcg ctgactaccg
    12541 cactgcgtcg cgaaccacgg ctgcgcttta cgctaacaga agtgaatgat ctaccggtcc
    12601 ggcaaacgcc gatgtttacg ctgctgcgtg aggcgggatt ttcaagttcg ccacaagggc
    12661 tggattgggg atagagaaag gactgacgga tgcccgttcg catccgtcag tattgcagga
    12721 cggattattc cgcgtcgggc tcttcagact tgtatttagc ggcagtttct ttgatcagct
    12781 gctgcagttc gccacgctga ttcatttcga tcacgatatc acaaccgccg accagctcgc
    12841 cgtcaaccca cagttgcggg aaggtcggcc agttagcata tttcggcagt tccgcacgaa
    12901 tgtccggatt ctgcagaata tcaacatagg caaaacgttc gccacatgcg gcaagcgcct
    12961 ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc atgtacagca
    13021 ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat agtggtgctc attgtcttgc
    13081 ttcctcaaac ttctttacgg cagtaatctg acattgtagc gggtcagtgc ggcatcggaa
    13141 aataacattt tcatcacgct tttgctattt tatccctttg ctctatttgt tgcatttcaa
    13201 atattcgttt cttctctgat gccggcaaac tggcgtattt ataaccattt ttagctttca
    13261 ctgctgcatt tttttgcgct cgccaacgaa acgtattttt taacaataaa agctattaac
    13321 tttctcttct tctatgcatt agaatcatca agttttgtaa atcagacgca ggcatgatag
    13381 acctgcctta acagagggac gctcagtggc gcggataaac cgtatttcga tcacgctctg
    13441 tgctttactt tttaccaccc tgcctttaac gcctatggcc catgcttcaa agcaagccag
    13501 ggagagttct gctaccactc atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac
    13561 caccaaaaag acccagaaaa cagcgaaaaa agccgccagt aaaagtacga ccaaaagcaa
    13621 aaccgcttct tccgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag
    13681 caaacacgcc gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa
    13741 gggctataaa tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaactc ttgtcgacgc
    13801 gcacaaagcg aaggtgcaaa aagctacgaa agtggcaatg aataaactga tgcagcaaat
    13861 tggtaagcca tatcgttggg gtggcagctc accgcgtacc ggttttgatt gcagcggcct
    13921 ggtttattac gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat
    13981 gtatcacctg cgtgatgcag cgccaatcga acgtagtgaa ctgaaaaacg gcgacctggt
    14041 ctttttccgt actcagggac gcggcacagc cgatcatgtc ggcgtgtatg tcggcaacgg
    14101 caaatttatt cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga
    14161 ctactggcag cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta
    14221 aaactttacc ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt
    14281 tgctacccta tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga
    14341 gagtagcaat gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc
    14401 acatttctgc ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta
    14461 acctgaacaa cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc
    14521 gcagctctga aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact
    14581 ggaactgcct ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg
    14641 ccgcatcttt tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa
    14701 actttggttc tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt
    14761 caacctctaa cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg
    14821 tctgggaaca cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact
    14881 tctgggcgct ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg
    14941 caaatgcagc attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca
    15001 gcgtattcgg caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc
    15061 tgcgtttttg tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga
    15121 cataaacacc agcaataatg ccagtcccgc gacaatcgct cccatcaccg gcacaaagct
    15181 gtatcccagc ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag
    15241 attaaaggca ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacacgcat
    15301 cacgcgcatc tgtaacggcg gtacgaccgc aaaggttgct gcgccccaca ccaccatgct
    15361 aatagctgcg ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcaa
    15421 caacaacaaa aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag
    15481 atagttaccg atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg
    15541 tgttgcgtgg gtaatacttt gcagtaccgg agagatatag gtgtagagag taaacattgc
    15601 accagctccc agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc
    15661 cagctctttt ttcacttcag gtcgtgcccc tgcaccacct ttaggtaatg agaagaacag
    15721 acttaccatt gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat
    15781 ggtttcaccc aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat
    15841 aaacatagtg gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac
    15901 tgaacccaaa ccaaaaaatg ctccgtgatt caggctggtc aaaatgcgtg aaagcatcag
    15961 ggtcatataa tccggcgcga tggcggaaag tacgttgccg agcgtgaaaa ttgccatcag
    16021 gaaaatcaac gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc
    16081 caccattacg ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc
    16141 cacaccgcgc gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc
    16201 aaacgcgcca atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct
    16261 cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa
    16321 atgaaaataa caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg
    16381 cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc
    16441 tacgatcaaa ccgaggacga taatcgttgt taccagtgaa aatttaaggt cggtgctcat
    16501 caagttttct ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt
    16561 gatgaaaaaa tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc
    16621 aagatcggcc aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga
    16681 cgctccgtgt cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag
    16741 gcaaacgttt accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat
    16801 ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt
    16861 aatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa
    16921 agaattacac tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc
    16981 tatcggtttg ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcggt
    17041 tgaaaaaaat tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct
    17101 tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt
    17161 gatgtgttct gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc
    17221 aatggtggtg atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa
    17281 cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat
    17341 cggcgtcatc cccggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat
    17401 gaaggcgatg gaagaagcga tgatcaaggt gccggaaagc tggattgttc agggtgactt
    17461 tgaacccgaa tctggttatc gcgccatgca gcaaatactg tcgcagccgc atcgccctac
    17521 tgccgtcttc tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat
    17581 gggtctgcgc gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcccg
    17641 ctattttacg ccggcgctga ccacgatcca ccagccaaaa gattcgctgg gtgaaacagc
    17701 gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt
    17761 gcatccgcgc ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg
    17821 ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc
    17881 actatcgccg agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca
    17941 tgtcagacaa catgccgaat ccgggaaggg gttttccaat tcaagtgcta cccacttccc
    18001 ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga
    18061 gatacaggtt gccgatgatt cccagtcagg cacgacaact cttttttggt tatccagcaa
    18121 ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt
    18181 gcgcaacgta tcatcgctga atgggccatc catcaacgcc agcgggtggt ggctggcaac
    18241 aacgcaactc cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat
    18301 cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc
    18361 gttgaacact tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac
    18421 gatcatctgc cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc
    18481 gcgccagccg ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac
    18541 agagcgccct tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg
    18601 tcgttcaaag agcggcaccg ccagccactc ttccagctga cgcacggtat agctgaccgc
    18661 agaaggaacg cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc
    18721 atcaacaact tcgagtgaat attctgacca catagtctac ctgcaaaatt tttgaaacca
    18781 gtcatcaaat attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt
    18841 taattgagag atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc
    18901 gggtttgagc gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat
    18961 acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc
    19021 cggttttgct gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc
    19081 ggtgttatta atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga
    19141 aaacgccgct acgctgctgg tattgcgttt tgtacaggct gtgggtgtct gcgccgcggc
    19201 ggttatctgg caagcgttag tgacggatta ttatccttca cagaaagtta accgaatttt
    19261 tgcgaccatc atgccgctgg tgggtctatc tccggccctg gctcctctgt taggaagctg
    19321 gctgctggtc catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt
    19381 gctgattctg cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga
    19441 tggtctgacc tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata
    19501 cgcggcctgt tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga
    19561 aatgggctac agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct
    19621 gattggtggt tatggctgtc gcgccgcgct gcagaaatgg caaggcaagc agttattacc
    19681 gtggttgctg gtgctgtttg cagtcagcgt cattgcgacc tgggctgcag gcttcattag
    19741 ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc
    19801 gatctacccg attgttgtcg cccaggcgct gcgtcccttc ccacacgcaa ctggtcgcgc
    19861 cgcagcgttg cagaacactc tacaactggg tctgtgcttc ctcgcaagtc tggtagtttc
    19921 ctggctgatc agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt
    19981 agtgctggtc gctctgggtt acatgatgca acgttgtgaa gaagttggct gccagaatca
    20041 tggcaatgcc gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt
    20101 aggctgctaa caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt
    20161 gttaaagcta cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg
    20221 gatggtcatt tcggcaaagg ttcctccttt ccctctgttc tacgtcggat tatagactcg
    20281 cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc
    20341 tgatcaccga ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta
    20401 ccggatgaca actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt
    20461 aacggttctg ccccggcgga tattcgtgtg aaaaaccccg atttttttaa acgcgttctg
    20521 caagaaggct ctttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga
    20581 ctggatatgt tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat
    20641 ttcaaagaca cgctgcgtat tgccagcgct cgtctcttca atctgcagag taaaaaacgt
    20701 gcctggatag tcggcaagga gcattacgat ttgggtaatg acttgttcag ccgcatgctt
    20761 gatcccttca tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc
    20821 cagcaggcga agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta
    20881 ctggatattg gctgcggctg gggcggactg gcgcactaca tggcatctaa ctatgacgta
    20941 agcgtggtcg gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgtgaa
    21001 ggcctggatg tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt
    21061 attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg
    21121 gtggtggatc gtaatttgaa accggaaggt atattcctgc tccatactat cggttcgaaa
    21181 aaaaccgatc tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg
    21241 ccctctgtac gccagattgc tcaatccagc gaaccccact tcgtgatgga agactggcat
    21301 aacttcggtg ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca
    21361 tggccagaaa ttgcggataa ctacagtgaa cgctttaaac gaatgtttac ctattatctg
    21421 aatgcctgtg caggtgcttt ccgcgcccgt gatattcagc tctggcaggt cgtgttctca
    21481 cgcggtgttg aaaacggcct tcgagtcgct cgctaaaggc tattctatcg ccccctctcc
    21541 gggggcgatt tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc
    21601 cagcacacgt tctaccgtat ctaccactgc ctgagtttgt ggatcaattt caatgttgac
    21661 gcgtgcgccg agttttttct tcccaagagt cgtgcgttcc agagtttccg gaattaaatg
    21721 gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt cgatgccaat
    21781 aaatcctttg tacaggatat atttcatcaa ctgactatcc tggactttaa accagatctg
    21841 gcgattattt tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga
    21901 cattaagtgt ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc
    21961 ccccactttt aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact
    22021 gacatggttg ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc
    22081 acctgtttcc aggccgtcca gcatgtggtc gggtaactcc accacatgcg tacgaaaatt
    22141 tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact
    22201 tacaactcct gaaatcagtt aagacattct gttcagcaca atagcaggtg gcaaacgccc
    22261 ttaccagtga aggggtaaga atgactattt tttcactgga gaattaataa atcctcgcta
    22321 caatagactg aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc
    22381 tctcatataa ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct
    22441 gttattagca ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt
    22501 tgtcgatacc gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg
    22561 tacttctatc tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc
    22621 ggttatcgcg caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca
    22681 aggtttctgg ctggcaggtt ttgtttccgt tctcattatg cttgtgctgt ggaatgcagg
    22741 ttacattatc cgctccatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta
    22801 tctgcgtgcg ttgttgtggg gcgcgccggg ctatctgttc ttccaggttg cccgtaacca
    22861 atgtgaaggt ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt
    22921 gaacattccg gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg
    22981 cgttggttgt ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc
    23041 ttacattaaa cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgcgaaacc
    23101 cgatcctgcg gttatgaaac gactgattca actcggtttg ccgattgcgc tggcactatt
    23161 ctttgaagtg acactgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga
    23221 tgtcgcagga caccagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc
    23281 gctggcggca gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga
    23341 tgcgcaaacc gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc
    23401 catattcacg gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt
    23461 tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactcaat
    23521 ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat
    23581 cacctttacg gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga
    23641 tctggtcgtt gaacctatgg ggccagcagg cttctggata ggctttatta ttggcctgac
    23701 gtcggcagcc attatgatga tgctgcgtat gcggttcctg caacgtctgc cgtcagttat
    23761 cattctgcaa cgagcatccc gctaataaag acaaggcgca accttcacgg gttgcgcctg
    23821 tgtttttacg caggctggag cgttgcgcca atcccgtctt cgtctggctg taatttcaga
    23881 gcgttgcaca gagtttgccg actggcatct gttatctcaa cgtgtatatc cccggtaact
    23941 tcccctttca cattgatgaa gtcaggtgag atttctttcg cactggtttg caggcaaatc
    24001 gctccctctc cgcgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatc
    24061 agccacagtt ttccgccatt cagattgatg ttaccaacaa taaaagtgac accgtcacct
    24121 tttaccgtac tgccgtcgag aatcaacaca ccgccctgct gtacagtaat cgttgattgt
    24181 tgcagatctg cgccatagcc aataatcatt gaagcgccgt ccagtaatgc aacactcccg
    24241 gtcagttcaa ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt
    24301 gacaacagga tcactgttcc accttgtatt accgcatttt ccagcgtacc ggcatgcact
    24361 tcagcccgac cacccacatt cacggacagg ttttgcgtct taccggaact gtagagctga
    24421 aggccatccg tccccagacg ataaatggat ccattctcaa caatagtgtc agagtcttca
    24481 ccaccgagat tattcatggt gccaccagca agcaacgtat cactggcttt cccggccagc
    24541 ataaaaacgc cgccctgatt aatctcacaa ttgctggctt ccccacctgg atacaccaac
    24601 aattcaccac cttcatctac cacgaccgct gaggttgtcc cattaaccaa caggccgccc
    24661 tcttgatcga gaatgatttt ttccgcgcga tgtccttcca gtacacgcag gttaccgcca
    24721 ttttccagca acaaaccgca ggcataaccc tgatcaacgc tgaattcacc ttcgggatgg
    24781 cggccattaa ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata
    24841 tctgtggcaa tggcaccgtc gctgaccgat agcgtgccat tgttaccgta ctgtgtgcat
    24901 ttggcgatcc caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg
    24961 gctcttccgc cagcttcaac aatcaactcg ccttcagtaa gttgcgtcgc gccaatgacc
    25021 tcgccggtct taacaatttg cacattcccg tcgctcatca agtttttcgc atcagatccc
    25081 atgatttatt cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg
    25141 gaggatttat ccacgacaga tttgagatgg tggcaaacaa ctctgtttaa actctgatac
    25201 acgaattatt gggttgtatc agatgtaaat gcgatcctga ataaaaatcg cccttgcaaa
    25261 tcaacaaaat atagccaatt tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa
    25321 ttacgttttg cctcttgcca ccttcccact ctgccgctaa tattcgtccc cgttgtcacc
    25381 tacaacgttg cgttcatagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt
    25441 tggttcgagt ccaattgaac gcaccatcct gcgtccgtag ctcagttggt tagagcacca
    25501 ccttgacatg gtgggggtcg gtggttcgag tccactcgga cgcaccagat tttcttaatc
    25561 tggtcttctc ctttttccct ctgtttcttc tctgtatcca atacgttaaa agatttacac
    25621 tgtcttcgta tgcgttatca gaaggagaat cgctatggca actttgttac aacttcattt
    25681 tgctttcaat ggcccatttg gcgacgcaat ggctgagcag cttaaaccgc ttgctgagtc
    25741 gattaatcag gaacctggtt ttctgtggaa ggtatggacc gaaagtgaaa agaaccacga
    25801 agccggtggg atctaccttt tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca
    25861 cactgcccga ctgaaaaatc tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa
    25921 tgagccactg agtcaaatca atcaggcaaa actcgcctga cagaatttaa tcaagggcgg
    25981 ttagcgccct tttcatccct gtctgaaatt tctcaaattc taaaaatctc aaccaaactt
    26041 atctgataac actaaattcg aaagaatgcg tacaggtaag taacaatgaa aaaaattgct
    26101 attgtgggtg ccgggcctac ggggatctac accttattct cgcttctaca gcaacaaact
    26161 ccactttcta tttctatctt cgagcaggct gacgaggccg gtgtcgggat gccatacagt
    26221 gatgaggaaa actcaaaaat gatgctggca aatattgcca gtattgaaat accgccgatt
    26281 aattgtacgt atctcgaatg gctacaaaag caagaagcca gccatctcca gcgttatggc
    26341 gttaaaaaag aaaccttgca cgatcgtcag tttttaccgc gaattctgct gggcgaatat
    26401 ttccgcgatc aatttttacg attagtagac caggcacgac agcaaaaatt tgcagtggct
    26461 gtttatgaat catgccaggt taccgatctg caaattacaa atgctggcgt catgctcgct
    26521 acaaatcagg atttacccag cgagacgttt gatttagcgg tgattgccac gggtcacgtc
    26581 tggcctgatg aagaagaagc aaccagaacg tattttccca gtccgtggtc aggcctgatg
    26641 gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat
    26701 gcggcaatgg cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc
    26761 gtttttaacc gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt
    26821 attttacccg aagccgattt ctattgccct attccctacg agcccttaca catcgtcacc
    26881 gatcaggcat taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt
    26941 agattgatag tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta
    27001 gagagcctga atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac
    27061 ccatttgact gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat
    27121 actgttccct ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct
    27181 catctgaatg aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat
    27241 aattatgcgg caatcccttc agagtctatt cgtcgcctac ttgccttacg tgaagcggga
    27301 atcattcata ttctcgccct cggtgaagac tacaaaatgg aaattaatga gtcgcgcacc
    27361 gtcctgaaaa cggaagacaa cagctactcg tttgacgttt ttattgatgc ccgcggacag
    27421 cgtccgctta aagtgaaaga tattcctttc cctgggctac gcgagcaatt acagaaaaca
    27481 ggggatgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
    27541 ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg accagccttt cgttcaggga
    27601 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
    27661 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
    27721 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
    27781 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctggcccg
    27841 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
    27901 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
    27961 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
    28021 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
    28081 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
    28141 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
    28201 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
    28261 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
    28321 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
    28381 tccgtatatc cttcctcaat cagtgcatga tgaatatcgg cataactacc gccgcgtggg
    28441 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
    28501 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
    28561 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
    28621 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
    28681 acggcttgcg gatagagaca cagcagcccc gtcaatagta acaacggcag caatccatac
    28741 ataacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgggt tgttgccggg
    28801 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
    28861 cgctccagcc acccctgacg acgaatgcga tagtggtgac cattacccca aacggcattg
    28921 atcagcacaa agccgagcca gcatgccagt aacaaaaatc cgcaaacttc atgcaccgca
    28981 accagacttt taaccgcagt tgcgcccacc agcgcaaagt gatttatcag cccacttgcc
    29041 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgcagtaa
    29101 agataaatct tctcaccata atcggtgact gatttcttca cgccacgagc acgaagcaag
    29161 gcatgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
    29221 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
    29281 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
    29341 gatacagatg cggtttcccg gcaccaggta gctgatattg atagtattta ttttgctgca
    29401 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccgggcagg
    29461 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
    29521 tatctgccac tttagtgaca gggttaaggt agcgaacctg gtacggacaa gcgccaatgc
    29581 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
    29641 acgacgcccc tgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
    29701 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
    29761 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
    29821 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
    29881 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
    29941 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
    30001 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
    30061 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
    30121 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
    30181 cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata tggcgcttgc atcagcatgg
    30241 cgactttgta aggacgagga tatgtctggc actcaaaacg aacggcgtgt gcaatggcgc
    30301 gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct
    30361 gcgttgagta atagtaatca tcctggcttc ccgtcataca agcgatatca gcgtagtctc
    30421 cgttctgctt aatgctttcc agcaaaactg acagctcctc ttctgcaacc gaatagggtg
    30481 gcgagagaaa cacggcgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact
    30541 ctgccaggat ttcagccggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct
    30601 gtaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatccc
    30661 gatggttcat ctcacccatt ccttacacag gcagtagatt gtgtgctgcc agatcggcgg
    30721 caatatcttc cagccccaga cgctgcaatg tttcgcgggt gggacaacca agctgtggat
    30781 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
    30841 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaatacc caggaacaga
    30901 taagatcgtg ttcattacgc atatctttgg tttgcataag ttttaccgtg taggcacgat
    30961 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg
    31021 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
    31081 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
    31141 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
    31201 tggtttcatc gtaggcatct tcagaaccaa aaagctcttt cgccacttca cgctgcagtt
    31261 tcaatggcaa acccgaacca ataaaattga tatgggtatg cgtcatgcag tcacggttga
    31321 acatacagtt aacaatggaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
    31381 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttca cccaaattcc
    31441 agcgttctgc gatggcatat gaaccatcag ccaggtgact cagctcaccc acacgatgcg
    31501 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
    31561 gaatttccgc atactcttca gcaggcagaa cacgcttgaa cacccctttg ctgtaacaat
    31621 aggtaaagtc gcgatgcagc tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
    31681 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
    31741 cgttcgggaa gatggtggta tggacaaagt tggctacaca agtgttacca cctgtgctgg
    31801 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
    31861 agtggcagcc actcattttt actgtgtatt tctctgccgc cggacctaag tcaaaaacgg
    31921 atttataggt acgaaagccg accgtgttct gattgcctgg cggaatttcc cccgtttcaa
    31981 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccaa cgtgacttgg
    32041 gatctgaata ctctgcccac gattgcggcg tacttggcac gacatggttg ttattcgcac
    32101 caataagttc agtcatcatg taatcgttga gacgcttcat ctcctgacga tcggcaatgt
    32161 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc ataattgcgc
    32221 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaacaaggt
    32281 tttccccagc ctgaccaata gccgccacac aggtttccgg actggtcaat cgacaaattt
    32341 cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
    32401 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
    32461 cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgacaacta
    32521 aatttccttt ggtaaaagta gaaagtgagg tgatatttac gcgagaacta cagggggcac
    32581 cagatccagt taatgggccg gtagcaaaga ctaatttatt cgcttcatcg aaaggtttcg
    32641 tgcctggcgg tacttcgtca tacataattt tgtagccgaa gcccatgcca ccgacaaaac
    32701 ttttaaactt actggaatct tcgagggtaa tatttcctgt cgtgagattg actcttaata
    32761 tattacctgt ccaaccgtta gccatgattt tttcctttgc aagattcaca cagtaatatc
    32821 tttccactcg ataattttta acgcccctgt tgggcaggcg tttgcgcatt caccgcataa
    32881 cacacatttc gaggattttt tactttcggt atttacggtg gccatcatcc acgggcatgc
    32941 cgtagtacag gcgctacagc caatacagct tttatgatcg acggtaatac agccttcttt
    33001 ctgctgccag gtaatcgcac caatcgggca gacgttcatg cattgcggct ctttgcattg
    33061 acggcaggtg tccgcggtat agttgagatc gccatacagg ccgccgccag agccaacccc
    33121 gttgtcgcca aagaaataat tgcggtggat tttgatacgg gagaagaatg tccctactga
    33181 gccatcgttg aagttggtac aagagatttc acagcggtgg caacccgtgc atcgcgctcg
    33241 ctgggtcacc agcacccctt tgggtgtatt tatcaaccct actgtgccgc tatcaatatc
    33301 ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca attgttagac cagcaaggcc
    33361 ttttcctgat atacgcaaga attctaatcg cgttaagcca atatctaata gtggacgatc
    33421 aaccgggttc atttatttcg catcctcttc gcaaacacag tgctgacaat tcagcagcct
    33481 gatattaagc attcaactaa tacgctgctg actctgtccc caacaccaga tattttccct
    33541 aacccgatag tgtatgagaa aagatttaac gccgataaac taatccttta tggttaggag
    33601 aaaaaataat gtgattattc tgagccctta acattgatcg ttatcaatta aaattacaaa
    33661 gcaagaaatt ataagtgaac tgatatttat tatcatttga aataaattta acttaagcga
    33721 caattatttg tgacgttcat tgcagaaaat aaaaataata ttatcgttat aaattaaaat
    33781 acataccgcg attcgtataa tttcagcagt tgttatttag aatgattaac tggaccgcaa
    33841 ctgaagcaag aatatcagga tgaagcttac cgcctcatcc tgaatggtca ttttaccagt
    33901 gcgttaatga cggtttccat cgcctgcttt tccagctcac tgcgctgaat gcgtttattg
    33961 gctaatgctg tacgtaatac gcctgcgata acaatatgct ttggttcctg ccccagatcg
    34021 cgcatttcca gcacaacttt accgacaact ctgcacattt ctcggtacag ttcatcctct
    34081 tttgtccgat ttcccattct ttatacctat ttatcatgcc aactatcagc atatatcaat
    34141 ctaaccaatt aaacaaaaat caaacaaaat cagacaaata acgcgataaa ttattttaac
    34201 tgtagcaatt gagcgatgat gtatttatac accggatgaa ctttcactta tcctcacact
    34261 gacaacttcg gcaccagacg ttgcgcaaac agtgaagttt ttgcgtaacc ttttccctgg
    34321 aacgttaaat ctttgataac aatttattgt ctaacaagtt gtatattttt tgaaacgctg
    34381 tttttgtttt ccttttggat taatttcagc gtataatgcg cgccaattga ctcttgaatg
    34441 gtttcagcac tttggactgt agaactcaac gactcaaaaa caggcactca cgttgggctg
    34501 agacacaagc acacattcct ctgcacgctt tttcgatgtc acctatcctt agagcgaggc
    34561 accaccactt tcgtaatacc ggattcgctt tccggcagtg cgcccagaaa gcaagtttct
    34621 cccatccttc tcaacttaaa gactaagact gtcatgaaaa agaccaaaat tgtttgcacc
    34681 atcggaccga aaaccgaatc tgaagagatg ttagctaaaa tgctggacgc tggcatgaac
    34741 gttatgcgtc tgaacttctc tcatggtgac tatgcagaac acggtcagcg cattcagaat
    34801 ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta tcctgcttga taccaaaggt
    34861 ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg tttctctgaa agctggtcag
    34921 acctttactt tcaccactga taaatctgtt atcggcaaca gcgaaatggt tgcggtaacg
    34981 tatgaaggtt tcactactga cctgtcagtt ggcaacaccg tactggttga cgatggtctg
    35041 atcggtatgg aagttaccgc cattgaaggt aacaaagtta tctgtaaagt gctgaacaac
    35101 ggtgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg tttccattgc tctgccagca
    35161 ctggctgaaa aagacaaaca ggacctgatc tttggttgcg aacaaggcgt agactttgtt
    35221 gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa tccgtgagca cctgaaagcg
    35281 cacggcggcg aaaacatcca catcatctcc aaaatcgaaa accaggaagg cctcaacaac
    35341 ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg cgcgtggcga cctgggtgta
    35401 gaaatcccgg tagaagaagt tatcttcgcc cagaagatga tgatcgaaaa atgtatccgt
    35461 gcacgtaaag tcgttatcac tgcgacccag atgctggatt ccatgatcaa aaacccacgc
    35521 ccgactcgcg cagaagccgg tgacgttgca aacgccatcc tcgacggtac tgatgcagtg
    35581 atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg aagcggtttc tatcatggcg
    35641 accatttgcg aacgtaccga ccgcgtgatg aacagccgtc tggatttcaa caacgacagc
    35701 cgtaaactgc gtatcactga agcagtttgc cgtggtgcag ttgaaactgc agaaaaactg
    35761 gatgctccgc tgatcgttgt tgcgacccag ggcggtaaat ctgcacgtgc ggtgcgtaaa
    35821 tacttcccgg atgcaaccat cctggcgctg accaccaacg aaaaaactgc acatcagctg
    35881 gtgctgagca aaggcgttgt gccgcagctg gttaaagaga tcacttctac tgatgatttc
    35941 taccgtctgg gtaaagaact ggctctgcag agcggtctgg cacacaaagg tgacgttgta
    36001 gttatggttt ctggtgcact ggtaccgagc ggcactacta acaccgcatc tgttcacgtc
    36061 ctgtaatatt gcttttgtga attaatttgt atatcgaagc gccctgatgg gcgctttttt
    36121 tatttaatcg ataaccagaa gcaataaaaa atcaaatcgg atttcactat ataatctcac
    36181 tttatctaag atgaatccga tggaagcatc ctgttttctc tcaatttttt tatctaaaac
    36241 ccagcgttcg atgcttcttt gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa
    36301 tattctcaac ataaaaaact ttgtgtaata cttgtaacgc tacatggaga ttaactcaat
    36361 ctagagggta ttaataatga aagctactaa actggtactg ggcgcggtaa tcctgggttc
    36421 tactctgctg gcaggttgct ccagcaacgc taaaatcgat cagctgtctt ctgacgttca
    36481 gactctgaac gctaaagttg accagctgag caacgacgtg aacgcaatgc gttccgacgt
    36541 tcaggctgct aaagatgacg cagctcgtgc taaccagcgt ctggacaaca tggctactaa
    36601 ataccgcaag taatagtacc tgtgaagtga aaaatggcgc acattgtgcg ccattttttt
    36661 tgtctgccgt ttaccgctac tgcgtcgcgc gtaacatatt cccttgctct ggttcaccat
    36721 tctgcgctga ctctactgaa ggcgcattgc tggctgcggg agttgctcca ctgctcaccg
    36781 aaaccggata ccctgcccga cgatacaacg ctttatcgac taacttctga tctacagcct
    36841 tattgtcttt aaattgcgta aagcctgctg gcagtgtgta tggcattgtc tgaacgtttt
    36901 gctgttcttc tgctgatagt ggtcgatgta cttcaacata acgcatcccg ttaggttcca
    36961 cggaatattt caccggttcg ttgatcactt tcaccggcgt tcccgtccgc acgctggaga
    37021 acaaggcttt aatatccggt gcattcatgc gaatacaccc tgaactgacg cgcaaaccga
    37081 cgctgtccgg cgcactggta ccatgaatga ggtattcgcc attaccatgc gcgaggcgca
    37141 gtgcgtaacg tcctagcggg ttatttggtc cggcaggaac gactggcggt aatttaatgc
    37201 cacgctccag cgaacgttga cgaatgcctg ccgtaggcgt ccaggttggg ttagggattt
    37261 tctgcccaac acgcgtttcc atcaccggcg tttccagccc ctgcaatcca atacctattg
    37321 gataaacctg cacaatattt tctcccggcg gataataata aaggcgcagc tctgcaaggt
    37381 taacgataat cccctgacgc ggtgcatcag gtaataacag ttgtgaagga atagttatcg
    37441 tcgtaccagg ttttggcacc ggggcgatag tgttattggc ttcaaggatc aacattgccg
    37501 cagtatcaaa acgtcgggca atagcctgaa ggtttttatc cccttcttgc accgtatacg
    37561 tttgattttg cccaaccagt cggcttccgg ttggtggtag cggataatca accgcccagg
    37621 cagcctggat ggcgctaaaa gcgccgataa gcgtgagtgt aagcaaagac gcgcgtttca
    37681 ttgtaaacct cctgtatttg ccggagactc acgctgaaac gtcggatggc gcttatgttc
    37741 acctgaaacc aaaacactcc tgtgcaggtc agtgtaaaca ttgaccatcc ggcaatgtga
    37801 gccaaccgga tgaaagctgt ccctttagtt tagctaagtg cagcggcttt ggcgcgaatt
    37861 gcgcgaatca tcgcttccag accttgtgaa cgagatgggg tgagatgttg ggtgagcgcc
    37921 attttttcaa accacggacg cacatcgaaa ttgacaatat cctgcggcgt catctgatcg
    37981 tagagaataa atacgaccgc aataagccct ttcacaatcg ccgcatcgct gtcgccctgc
    38041 aattcaataa ttccctgcgc attctggcgc atgacaatcc acacctgact ctggcagccc
    38101 tgaatgctat tttgtgggct tcggtcttcg tcgcgtaatt ctggcagacg ctggcccagc
    38161 tcaataatgt agagatattt ctcttcccaa ttggcgcagc gtaaaaaatt acgcagcaat
    38221 ttttctttat ccggcaataa agccatagtg cctccctgtt atcccagcag acggtgaata
    38281 cgttgcagtc cggtcaccag acgatccact tcttcatggg tgttatacat ggccagcgac
    38341 gcccgacaca tcgcagggac gttgtaatag gccattaatg gcatagcgca gtgatgtccg
    38401 gtacgcacag caatgccgta attatcgaga aaactgccaa catcataggc gtggtgttta
    38461 ccgagattaa aagcaataac gccaagcctg ttttgtgggc catagagagt gagatccggt
    38521 acagattcca gctgtgatag cgcataatgc atcagattct gttcatactc ggctatgcta
    38581 ttaagcccca gcgccgaaac atactccagc gccgcgccaa gaccaatgat gcccccggta
    38641 ttgggtgtac cggcttcaaa ccgccatggt gctttggtcc aggtagtgcc ttcactcagg
    38701 ctgacggtgg cgatcataga accgccccct tcccacggcg gcatctcctg caacaaggct
    38761 tctttcacat aaagaatgcc aattccggtg gggccataca gtttatgccc ggagaacacg
    38821 taaaagtcgc aatccagcgc ctgaacatcc accggatgat gcatcaccgc ctgagcgcca
    38881 tccaccagca cttttgcgcc atgctggtgc gcaagcgtga ttatttccgc cagcggattt
    38941 tctgttccca gcacgttgga gacatgagta attgccagca ggcgagtttt ctcatcaaac
    39001 agcgtaggca gcgtctccag ttgcagcgta ccgtcggggt tgagagggat cacacgcagc
    39061 tctgcgccaa cgcgtgcgca aagcatctgc caggggacaa tgttagcgtg gtgctccatc
    39121 tgactgatga tgatgttatc gcccgcccgc acattgctgt tgccccagct attggcgacc
    39181 agattgatcc cttccgtcgt gccgcggacg aacaccagct cttccgccga acgggcatta
    39241 ataaacagcg atgcccgctt gcgcacgttc tccattttct cggtcgcctg agcgcttaag
    39301 gtatgaatac cgcgatgcac cgccgcgtag ccatgacgat aaaactcggc ctcggcgtca
    39361 atcacctggc tcggtttctg tgcactggcg gcgctgtcga gataagccag cggcaaaccg
    39421 tttacctcac gcgaaagcac cggaaagtcg gcccgcactt tgtcgacgga aaaagtcatc
    39481 ttgcacctcc tggcagccgt tgaccgattc gggccagcac ctgctgttta agcccctcat
    39541 cacgcagtgc ttccgtcagt tcggcagcaa aggcgtaaat gatcatctgc tgggcatcct
    39601 gctgattgat cccgcgcgag cgcagataga acatctgttc atcatcaata cgccccaccg
    39661 tcgcgccgtg gctgcatttc acatcatctg catagatttc cagctgcggt tttgtatcca
    39721 cttccgccag tttgcccatc agcagattat tgttggtcat ctgaccatcc gttttgatgg
    39781 cgtgctgcgc gacgttgatc aaaccgttaa ataccgcgcg gcctttgtcg ctgacgatag
    39841 ttttgtgcaa ctgtcggctg ttacaaaaac ctttattgtg ttccagccag gtacgggtat
    39901 cacacacctc gtttttcacc ggcatcgcca ggctattgat ccgcagcgtg ctgttttcgc
    39961 cattgagttg cgtactggtg ttgtgtcgta acactgcgcc acccagcagg aaactatggc
    40021 taaatgcggt ggcatcgtca gccagcaaca aatcgttatg ggcaaagtgg tgactgaccg
    40081 ggttttcaaa cgccagcttg atatgctgca agtgggcgtt cgctgcgaca ttgatagtga
    40141 accgtgcgcc ggtgaagtga cgagcatcat taaggctgac aaaatgttcg atcaccgttg
    40201 cttcggcacc ttccgccaga tccagatgat gtcggtaatg ggcagtgttg acctcttcac
    40261 ctgccacgcc ctgggtaata tgcattaaca gcaatggctt tgccggccgt tgaccgcgct
    40321 tcacggcgat atgcgtcacg ctttgtgcca ggctttccgt caaatgcaga aacacttccg
    40381 cctgaatagc gtcgggtacc ccctgacggt cgtcgttaat gctcacttca tacccgctgc
    40441 cttcagtcgc atcgctcagt gctgacacgt aacgtccatc gacaaatacc agccgcacag
    40501 cgtctaacgt taacgctaag gcatctcgct gctgtgggga tatctctccc gcaatgctga
    40561 caaactggct attggtcagc ccttccagcg gcgtatattt ccagttttca tgtttacgtg
    40621 tcggcagtcc ggtacgcagc aattgttgta aatgctgctg tgcttgcggt gagcgttttg
    40681 tcccttcagc ttcaaacaag tgatgccact gttgcagcgc attactgctg ttcggtaagc
    40741 cagccataac cctgctcctc cagttgtttg accaacgtga aatcgccgga tttcacaatt
    40801 cgtccctgat atagcacatg aacgtaatca ggcttgatgt agtcgagaat gcgttggtag
    40861 tgcgtaacaa tgatgaatga gcgcttgcca tcacgcagcg agttcacgcc atcggcgacc
    40921 acttttaatg cgtcaatatc cagcccggag tccgactcat caagaatgca taactccggt
    40981 tccagcaccg ccatttgcaa aatatcgttg cgctttttct cgccgccgga aaaaccaacg
    41041 tttaccgaac gggttaataa atcttccggc atcttcagga gagcgatttt ctcttccatc
    41101 aaatcctgaa aatcaaagcg gtcgagcgtt tcctggccgc gatagctgcg caccgcatta
    41161 agtgccgttt gcaggaaaaa ctggttactg acgccgggaa tctccaccgg atactggaag
    41221 gccataaaga tgccttcgcc tgcacgatct tccggcgaca gcgcaagcaa atctttgcct
    41281 ttgaactcaa ccgtgccgcc cgtcacttca taatcttcgc gcccggcaag cgttgccgat
    41341 aaggtacttt tgcccgaacc gtttggcccc ataatggcgt gaacttcgcc gggacgaacg
    41401 tcgaggctta atccacgcag gatagcttta tcttccacgc tgacgtgtaa atctttaata
    41461 cttaacatgt ttattcctta tccgacgctg tgttcaagac tgatggcgag gagtttttgt
    41521 gcttcaacgg caaattccag cggcagttct gagaacacgt ctttgcagaa accgttaaca
    41581 atcatcgaga tggcgtcttc ttcgctgatc ccgcgttgca ggcagtaaaa cagttgatct
    41641 tcaccaatac gtgatgtcgt tgcctcgtgc tccagttgtg cgctgttatt gcgacattcg
    41701 acatacggga aggtatgcgc cccacaatta gcgccaatca gcattgagtc gcactgagtg
    41761 aaattgcgcg cattggttgc cgtcggcatg attttcacta agccgcgata actgttctga
    41821 ctatgtccgg cagagatccc tttcgagata atggtcgatt tggtgttttt accgatgtgg
    41881 atcatcttgg tgccggtatc cgcttgctga tgaccgctgg tcagcgccac tgagtaaaac
    41941 tcaccaatgg agttatcgcc gcgcaaaatg cagctgggat atttccacgt aatcgcggac
    42001 ccggtttctg attgcgtcca tgacattttg ctgttttcgc cttcgcacaa agcacgcttg
    42061 gtgacgaagt tgagaatacc gccggtgttg ttatcgccag ggaaccagtt ttgtaccgtg
    42121 gaatatttca cctcggcgtt tttatggatg atgacttcca ccactgccgc gtgtaactga
    42181 tagctgtcac gcaccggagc ggaacagcct tcaatgtagc tgacgtagct gtcttcgtcg
    42241 gccaccagaa tggtgcgctc aaactgcccg gttttttccg cgttaatgcg aaaataggtg
    42301 gaaagttcca tcgggcagcg cacgccttta ggcacataaa taaacgtacc gtcagaggct
    42361 accgccgcat taagcgcggc aaagaagttg tcattccccg gcaccacggt gccgagatat
    42421 ttacgcacca gttccgggtg atcgtggatc gcctcaccaa aggaacagaa aataattccc
    42481 tgctccgcca gtttttcgcg ataagtagtg gcaaccgaaa ctgagtcgaa aatggcatcc
    42541 accgccacct ctttgccttc ccgcacggga acgcccaact gctcaaacgc cgcctccacc
    42601 tctttactta aaaaggcgtt cgcgccagtt tgctgcaccg cgccaggttc agacgcgcaa
    42661 gtgtcgtcac aattaccgca cgatggtgct gagtagtagc tgtaatcctg ataattcagc
    42721 ttgtcgtagt gcgctttcaa ccagtgcggt tcttccatct ccagccatgc gcgataggcg
    42781 tttagacgaa acttcagcat ccactccggc tcattacgct tcgccgaaat tgcgcgcacc
    42841 acctcttcgt ttatcccctt tgccagctca tcggtggcta actgggtgaa gaatccttct
    42901 ttataattca gcgggccgcc ggtccaggtt ttgacatcgt cagttgcttc agtattacga
    42961 gacatagtac cgcctatacc ccaaagcttt cgccacagcc acattcattc tgggctttag
    43021 ggttgtgaaa tttgaatatc tgattaagtc cttcacgaac gaaatcgact tccgtgccat
    43081 caataaacgg catcgcttgc agcgggacaa acagcttcgc gccgtcgtgt tcaaacagca
    43141 gatcgtcttt gtccggctcg ctaacactgt cgagcacata gccaaagccc gcgcagcccg
    43201 tttgcttcac gcctaagcgc acgccgacca tacccggctg ctttgccacc agctcacgga
    43261 tgtgtatcgc cgctgcgggt gtcagcgtta agccttgcca ggcgaaatct tgtgggttaa
    43321 aggttcctga atgcatgtcc atcgatttac ctcacttcat cgctttcagc gtataacagc
    43381 atgttagtga taatgattat cagtacaacc cagcaaacgc aggggcttta ccgtaaaaca
    43441 tgctttttgc ctgcttttaa taagcataga ccctgatgtg tgggttaaca ggcacgctaa
    43501 gaattaggta tctcattgtt agataatggt tattatctaa ggagcattaa ggctgtgatg
    43561 aaaaagaaaa agttgtattg aaaatgacta tttaagagat aggtaaaaaa gtgcagcgtt
    43621 cagaaataag aaaaccctta agtctgtgca acacaggctt aagggtttct accccatccg
    43681 gcgcttatct ccggcactct cagtggctta gctcttgaag gggcgataag aataatctca
    43741 taaagctaac ccgccgtttt aacacaaact gcgattagta ttatttttga acaatatcag
    43801 gcggtagata agcagtatta agaaggtcat cgaacctgga cggaggttaa tccaggtcga
    43861 tttggcgaac ttgcggcatt aagtcaggat caatgcacgc ccagacgcca ggcaaagtag
    43921 atttcttctt ttaattcagc agaagagaga gtaagcaggt cagcaaattc aagttctagt
    43981 tgtttcagac gtttgagata ttgggcaggt gaaagattgc tctggtcacg gcgtaaaaat
    44041 tcaatggcca gctgggtggg atcaagttga gtagacatag catcctcgct tttagacaag
    44101 acctgcacag tataccaccg tttactgtgc agataatgac caaaagcaat atgcgtcaca
    44161 cttttctggt gacaacgtca caaaatggcg gtcgtcaatc gtgacgaaca gcacaaacgc
    44221 cctttctcat cgaagatttc aatctgccag acctggtgac gcgaaccgag atgcaacggt
    44281 ttgcataccc cgcgcacccg cccttctcgt gccgagcgga cgtggttagc attgatttcc
    44341 agaccaacca ctttttgctc accttcggta cataaataac cggcaacgga accgatactt
    44401 tcggccagta ccacggatgc gcctccatgc agcaacccga aaggctgctt tgtccgcgag
    44461 tctactggca ttgtcgcttc aagggtgtca tcaccaatat gttcaaagcg aatatccagg
    44521 aaccccacca tgtttccttc acccatagca ttcagtgctt ccagggtgat tttccgtttc
    44581 catatcattt aataatctcc agtaaagcct gcacaggatg gcgtaccccc gtgccttcaa
    44641 cccgttttac ctggctacgg caggaatatc cggtcgccag acagcggtta cgcggcagtc
    44701 gctgcatagc ctgatgccag gataactcat agatcccgag cgaattttta tggtttttcg
    44761 cttcatgtcc gtaagtccct gccatgccgc agcaacccac gctgacattt tccagtttcg
    44821 cgccaaaacg ggcaaatatc gcggcccatt gtgctggcgc acccggcaag gcggtaactt
    44881 cggtacagtg accaaagaaa taccatgatt caccgctgac tgtagccact ggctgtgact
    44941 caagtgcgct tgccagccat tcattcgcca gtaagacgtt aaactcgcca cgttcctcgc
    45001 ccagcgccag tttatattca tcgcgataac aaagtaccag cgccggatcg acgcccacca
    45061 ttggcatacc cagcttcgcc atacggttga ggaaatccgc cgtctttttc gccgtcttcg
    45121 caaaacgatt aagaaaacct ttaatatgct gggctttgcc atttggcgaa aatggcagta
    45181 acacaggctg gaaacctaat ttttcgacca gacggacaaa atccgccacc acttgcgcat
    45241 cgtaatagct ggtaaagggg tcctgcacca ccaacactgt gcgcgctttc tgctctgcat
    45301 tgagagattc aagctgttcc agcgtcatgt ttgccgagcg atgccccacc atttgttgtt
    45361 gtagcgaggg gaccgacagc agcggcaaat caaccatgcc gatatgtttt tccgagagtt
    45421 tgcgcaccag cggctggtta atgaagaagt taaaggtttt cggcgcgcgt gccatcagcg
    45481 gcgcgtagct ctcgaccgta gcgacgaggt ggtcgcgcag cgggcgtaaa taacgggtgt
    45541 gatagagctg cagaaaacga gagcgaaact ccggcacatc aattttgatg gggcactggg
    45601 tcgaacacgc tttacaggcc agacagcccg acatcgcctc tttgacttcg tgtgagaagt
    45661 catattcgcc tttattcgca tgccagctat tgcgcgtgcg ggcaattaac gtccgcaaac
    45721 tgacgccgga ttcaggcagt tcttgttcca gtttgagtgg atcaacgccg cgatccgcca
    45781 acaaacgcag ccattcacgc accagcgttg cgcgcccttt cggtgaatga atccggttct
    45841 gggtgatctt catcgacgga cacatcggac tacgggcatc aaagttgaag cataaaccgt
    45901 tgccgttaca ctccatcgca ccgcgccact gctggcgtac cgcaatgggg atctgccgat
    45961 cgaatgtacc gcgcttcacc gcgtccactt tcatcatcgg cgcatcgaga ccttctggcg
    46021 ggcaaatctt ccctgggttg agtcggttat gcgggtcaaa tgccgctttc actttgcgca
    46081 gttctgcaaa aagttcctca ccgaaaaacg ccgggctgta ttcagcgcga aaacctttgc
    46141 cgtgctcgcc ccacaacaaa ccaccgtatt tcgcagtcag cgccactacg tcatcagaga
    46201 tttgcttcat caaaatctct tgttgaggat cgcacatatc cagcgctgga cggacgtgca
    46261 aaacacctgc gtcgacgtga ccgaacatac cgtagcttaa gccgtggctg tcgagcagcg
    46321 cgcgaaattc agcaatataa tccgccagat gttccggcgg tacacaggta tcctcagcaa
    46381 acggaattgg cttagcggca cctttggcat tgccaagcag accaacggct tttttgcgca
    46441 tcgcatagat acgttcaaca cccgccagct cgcggcacac ctgccagcca atcacacctg
    46501 cctgttggct gacaatcagc tcatcaagcc gcacacagag tgcattaact cgctcgtcaa
    46561 tcagcgcctc atcatcacca gcaaattcca caatgttcag tccgagcatc tctttgtcag
    46621 gcacatcggt aatcaactcg ctgacggaat gccagacaat atcttcccgc gccagattca
    46681 gcacttttga gtccaccgtc tctaccgaaa gcgcccgcgc ctcaaccata aacggcgcgt
    46741 tacgcagcgc agagtcaaaa gagtcatatt tgacattcac cagacggcgc actttaggca
    46801 agcgcgtaat atccagccgc gcttcggtaa taaaggccag cgtcccttct gaacccgtca
    46861 gaatgcgcgt caggtcgaac tcggtcatct catcgttaaa gacatgacgc agatcgtaac
    46921 cggtaagaaa gcggttaagt ttggggaagt tgtcgatgat taactggcgt tgctgacggc
    46981 aacgttgata aaccgtgtta taaattcgcc cgattgtggt attggattta cccagcgttt
    47041 ccgccagttc gacgggtaaa ggttgcgtat cgagaatatc gccccccaac aacaccgcgc
    47101 gtacgccaag tacgtgatct gacgttttgc catagaccag cgatccctga ccagaagcat
    47161 cggtattgat catcccgccg agcgttgccc ggttgctggt cgaaagttct ggtgcaaaaa
    47221 agtagccgaa cggtttcagg tactgattga gttgatcttt tatcaccccg gcctcaacgc
    47281 gcacccagcc ctcttcaggg ttaatttcga tgatgcggtt catatggcgg gacatatcaa
    47341 caataatccc ctggttgagc gcctgaccgt tagtgccggt gccgccgcca cggggggtaa
    47401 agatcagcga tgaatagcgt tcctgcgcgg caagacgggc gatcagcgcc acatctgcgg
    47461 ttgaacgcgg aaataccacc gcatcgggga gaagttggta aatactgttg tcggtcgaca
    47521 ttgtcagacg atcggcataa cttgtcgccg tatcgccggt aaaaccttgt tgctccagct
    47581 cttgcaaaaa attaagcacc agttgaacga cgccgggtgc ctgggaaatc tgtggaatca
    47641 ttatattgac cctttcctgc ggtctgtgat gtaggtcgat acactattct ttcaggcggc
    47701 tgcaatagcg cactgaaagg tgatgtttat ttactctatg gatttcgagt tgcaggaagg
    47761 cggcaagcga gtgaactcca ggagcttaca atagtaagtg actggggtga acgaacgtag
    47821 ccgcagcaca tgcaacttga aatacgacga gtaaatcgtt tgcgtgttgc ctgagttgtt
    47881 gtaccacatt tttttctaac acgcccatca gaattaaggg cagaatcggc ctgttaaaaa
    47941 ccgctgaaat tgctcatcat tatgcaggtg agtttcgcgt gttcacgtcg cgtcgacgat
    48001 ttgacgcaca aaaaaggtga aaagtagtta tggtaaatgt tcgtcagccc agggatgtcg
    48061 cacaaattct gctttcggtg ctgtttttag ccatcatgat tgtggcatgt ctgtggattg
    48121 ttcaaccctt tattctcggc tttgcatggg ccggtacggt ggttatcgcc acctggccgg
    48181 tattgttacg tttgcaaaag atcatgtttg gccgccgctc tctcgccgtt ctggtgatga
    48241 cgctgttatt agtgatggtg tttatcatcc ctattgcttt gctggttaac agtatcgtcg
    48301 acggcagcgg cccgctaatt aaagccattt ccagcggcga catgacgtta cccgatctgg
    48361 cgtggcttaa taccattccg gtgattggcg cgaagctgta tgcaggctgg cacaacttgc
    48421 tggatatggg ggggacggcg atcatggcga aagtccgccc ttatattggc accaccacca
    48481 cctggttcgt tgggcaggcg gcgcatatcg ggcgctttat ggtgcattgt gcgctgatgc
    48541 ttctcttcag tgccctgctg tactggcgcg gtgaacaggt ggcacaaggc attcgccatt
    48601 ttgcaacccg tctggcaggc gttcgcggag atgccgccgt actgctggcg gcacaggcta
    48661 tccgcgcggt ggcgctgggt gtggtggtga cggcgttagt acaggcagtg cttggcggta
    48721 tcggcctcgc cgtatccggc gtaccttatg caacgttgct aacggtgtta atgatcctct
    48781 cctgccttgt ccagcttggc ccgttgccgg tactgattcc ggcgattatc tggctctact
    48841 ggactggcga taccacctgg ggaacggtat tgttagtgtg gagcggtgtg gttggcacgc
    48901 tggataacgt catccgccca atgttaattc gcatgggtgc cgatttaccg ctgatcctga
    48961 tcctctctgg tgttattggt ggtttgattg ctttcgggat gatcggtctg tttattggtc
    49021 cggttctgtt agccgtttcc tggcgtctgt ttgccgcgtg ggtggaagaa gtcccgccgc
    49081 cgacggacca accggaagaa attctcgaag aacttggcga aatcgagaaa ccgaataagt
    49141 aatttctcat caggcggctc tgccgcctga ttgttaaccc ccgctaattg atatttgctt
    49201 gctcttcccc atattttcct gcttaccgcc attcagctgg tagtacctgt cgcaaattct
    49261 ttacagtttt taaactaatg agacgaatct gatcgacgca aaaagtccgt atgcctacta
    49321 ttagcttacg gttataaatc aacatattga tttataagca tggaaatccc ctgagtgaaa
    49381 caacgaattg ctgtgtgtag tctttgccca tctcccacga tgggcttttt tttaacattt
    49441 ttccgcttcg ctacctcgcc cctcactctt tccaataatg ttgcacaaga tactgtgact
    49501 ctcttcaagc cgtcagcagg ctgataatgt tatttatatt ttattgttta tttcatgcag
    49561 ccattacacg atgaacgctt atgaactcca ggcactccgc catatttttg ccatgactat
    49621 tgatgaatgc gcgacctgga ttgcccagac cggtgacagt gaaagctggc gtcaatggga
    49681 aaatggcaag tgtgccattc ctgatcgtgt agtcgagcaa ctgttggcta tgcgtcagca
    49741 aagaaaaaaa catcttcatg ccatcataga gaaaataaat aaccgcatcg gcaacaacac
    49801 catgcgtttt ttccccgact taaccgcatt tcaacgagtc taccctgatg gcaattttat
    49861 tgactggaag atctatcaat cggtagccgc tgaattatat gcacacgatc tggagcggct
    49921 ttgctaattt ttccacagaa aggaattgtc gttgttacaa caataatgaa tggatactga
    49981 cacaacatcg ctacactttt taaagcacct ttgctaagta gaacctatga aaaatcccta
    50041 ttaccctacc gcacttgggt tgtattttaa ttacctggtg catggtatgg gcgtcatttt
    50101 gatgagcctg aatatggcct cgctggagac actttggcag actaatgccg cgggtgtctc
    50161 gatagttatc tcatcgctgg gcattggtcg attaagtgtc ttgctttttg caggattatt
    50221 atccgatcgc tttggtcgcc gcccttttat catgctcggg atgtgttgct atatggcctt
    50281 cttttttggc atcctgcaga ccaataacat cattatcgct tatgtttttg gctttctggc
    50341 gggaatggca aacagttttc tcgatgcagg cacttatccc agtttgatgg aagcttttcc
    50401 acgctcacct gggacagcca atattttaat taaagcattt gtttccagcg gacaattttt
    50461 attaccgcta atcattagcc tgttagtgtg ggctgaactg tggttcggtt ggtcctttat
    50521 gattgctgca ggcattatgc ttattaacgc tctgttttta taccgttgta cgttcccacc
    50581 ccatccgggt cgtcgcttac ctgtcataaa gaaaaccacc cgctctacgg aacatcgctg
    50641 ttcaattatc gatttagcca gttatacctt atatggctat atctcaatgg caacgtttta
    50701 tctggttagc cagtggctgt cacagtacgg acaatttgtt gcagacatgt catacactat
    50761 gtcgatcaaa ctactcagta tctacaccgt gggttcgctg ctttgtgtat ttattaccgc
    50821 tccactcatt cgaaataccg ttcgcccaac aacattactg atgctgtaca cctttatctc
    50881 atttattgct ctgtttaccg tctgcctgca tcccacattt tatgtggtga taatatttgc
    50941 ttttgtcatt ggttttacct ctgctggagg tgttgtgcaa attggcctga cgttaatggc
    51001 tgaacgtttc ccctacgcta aaggtaaagc tacagggatc tattacagtg cgggcagtat
    51061 tgcgaccttt actattccgt tgattacggc tcatctgtcc caaagaagta ttgccgatat
    51121 tatgtggttc gataccgcca tcgctgccat cggcttttta ctggcactgt ttatcggctt
    51181 acgcagccgc aaaaaaacgc ggcatcactc gctaaaggaa aatgtcgctc cgggtgggta
    51241 atgcaatatt cttttcaggt catgcaagat cttacggata aataactctt tctgcgctaa
    51301 ctaaggaaaa tcgcgatcaa aaacaaacta tgacatgcaa tattcctgga aacataaact
    51361 ttatgccatg tacccaggga aaatcatctt cagtatagta attatgtaaa ccgtcggaga
    51421 acaatacgta cggtaacgaa attatctttc agcaaggagc tgtgaaaatg tctcaaaata
    51481 aggctttcag cacgccattt atcctggctg ttctttgtat ttacttcagc tatttcctgc
    51541 acggcattag tgttattacg cttgcccaaa atatgtcatc tctggcagaa aagttttcca
    51601 ctgacaacgc gggcattgcc tacttaattt ccggtatcgg tttggggcga ttgatcagta
    51661 ttttattctt cggtgtgatc tccgataagt ttggtcgtcg ggcggtgata ttaatggcag
    51721 taataatgta tctgctattc ttctttggta ttcccgcttg cccgaattta actctcgcct
    51781 acggtctggc agtgtgcgta ggtatcgcta actcagcgct ggatacgggt ggctaccccg
    51841 cgctcatgga atgctttccg aaagcctctg gttcggcggt catactggtt aaagcgatgg
    51901 tgtcatttgg gcaaatgttc tacccaatgc tggtgagcta tatgttgctc aataatatct
    51961 ggtacggcta tgggctgatt attccgggta ttctgtttgt actgatcacg ctgatgctgt
    52021 tgaaaagcaa attccccagc cagttggtgg acgccagcgt agctaatgaa ttaccgcaaa
    52081 tgaacagcaa accgttagtc tggctggaag gtgtttcatc ggtactgttc ggtgtagccg
    52141 cattctcgac cttttatgtg attgtggtgt ggatgcccaa atatgcgatg gcttttgctg
    52201 gtatgtcaga agctgaggca ttaaaaacca tctcttatta cagtatgggc tcgttggtct
    52261 gtgtctttat ttttgccgca ctactgaaaa aaatggtccg gcccatctgg gctaatgtat
    52321 ttaactctgc actggcaaca ataacagcag ccattatcta cctgtaccct tctccactgg
    52381 tgtgtaatgc cggcgccttt gttatcggtt tctcagcagc tggcggcatt ttacagctcg
    52441 gtgtttcggt catgtcagag tttttcccaa aaagcaaagc caaagtcacc agtatttata
    52501 tgatgatggg tggactggct aactttgtta ttccgctgat taccggttat ctgtcgaaca
    52561 tcggccttca atatatcatt gttctcgatt ttactttcgc gctgctggcc ctgattaccg
    52621 caattattgt ttttatccgc tattaccgcg tttttattat tcctgagaat gatgtgcggt
    52681 ttggcgagcg taaatttagc acccggttaa acacaattaa gcatagaggt taaaggagtt
    52741 aattatggat gttaccgcaa aatacgaatt gattgggctg atggcctatc ctatccgcca
    52801 cagtttatcg cctgaaatgc agaataaagc cttagaaaaa gcgggattgc catttaccta
    52861 tatggccttt gaagtggata acgatagttt tcccggagca attgaaggat taaaagccct
    52921 caaaatgcgc ggaactggta tatcaatgcc gaacaaacaa ctggcgtgtg aatatgttga
    52981 tgaattaacg ccagcagcca aactggtggg tgccatcaac accatcgtta atgatgatgg
    53041 ctacctgcgc ggctataaca ccgacggcac gggccatatt cgcgccatta aagagagcgg
    53101 ttttgatatc aaaggcaaaa caatggtgct gttaggggcc ggaggtgcct caacggcgat
    53161 tggcgcgcag ggggcaattg aaggtttaaa agaaattaaa ctctttaacc gtcgagatga
    53221 gttcttcgat aaagccctcg ccttcgcgca gcgggttaac gaaaacaccg attgtgtcgt
    53281 cacggttacc gatctcgccg atcagcaagc ctttgctgaa gccctggctt ccgccgacat
    53341 tttaaccaat ggcacaaaag tgggtatgaa accccttgag aatgaatcat tagttaatga
    53401 tatcagtctg ttacatccgg gactgctggt cactgaatgc gtgtataacc cgcatatgac
    53461 gaagttattg cagcaggcgc aacaagctgg ttgcaaaacg attgatggat acggcatgtt
    53521 gttgtggcaa ggggctgaac agttcacgtt atggactggc aaagatttcc ctctggaata
    53581 tgttaaacag gtcatggggt tcggtgcctg acaggctgac cgcgtgcaga aagggtaaaa
    53641 aatgaaaacc gtaactgtaa aagatctcgt cattggtacg ggcgcaccta aaatcatcgt
    53701 ctcgctgatg gcgaaagata tcgcccgcgt gaaatccgaa gctctcgcct atcgtgaagc
    53761 ggactttgat attctggaat ggcgtgtgga ccactttgcc gacctctcca atgtggagtc
    53821 tgtcatggcg gcggcaaaaa ttctccgtga aaccatgcca gaaaaaccgc tgctgtttac
    53881 cttccgcagt gccaaagaag gcggcgagca ggcgatttcc accgaggctt atatcgctct
    53941 caatcgtgca gccatcgaca gcggcctggt tgatatgatc gatctggagt tatttaccgg
    54001 cgatgatcag gttaaagaaa ccgtcgccta cgcccacgcg catgatgtga aagttgtcat
    54061 gtccaaccat gacttccata aaacgccgga agccgaagaa atcattgccc gtctgcgcaa
    54121 aatgcagtcc ttcgacgccg atattcctaa gattgcgctg atgccgcaaa gtaccagcga
    54181 tgtgctgacg ttgcttaccg cgaccctgga gatgcaggag cagtatgccg atcgtccaat
    54241 catcacgatg tcgatggcaa aaactggcgt aatttctcgt ctggctggtg aagtatttgg
    54301 ctcggcggca acttttggtg cggtaaaaaa agcctctgca ccagggcaaa tctcggtaaa
    54361 tgatttacgc acggtattaa ctattttaca ccaggcat
//
LOCUS       DAMZII010000018        52118 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZII010000018 DAMZII010000000
VERSION     DAMZII010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.018"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            15..557
                     /locus_tag="POA77_001022"
     CDS             15..557
                     /locus_tag="POA77_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6364491.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            640..1341
                     /locus_tag="POA77_001023"
     CDS             640..1341
                     /locus_tag="POA77_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6364492.1"
                     /translation="MKTCITKGIVTVSFTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1366..3966
                     /locus_tag="POA77_001024"
     CDS             1366..3966
                     /locus_tag="POA77_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDI6364493.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3957..5027
                     /locus_tag="POA77_001025"
     CDS             3957..5027
                     /locus_tag="POA77_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6364494.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5039..5581
                     /locus_tag="POA77_001026"
     CDS             5039..5581
                     /locus_tag="POA77_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6364495.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5589..6104
                     /locus_tag="POA77_001027"
     CDS             5589..6104
                     /locus_tag="POA77_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6364496.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6070..6807
                     /locus_tag="POA77_001028"
     CDS             6070..6807
                     /locus_tag="POA77_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6364497.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6918..7928
                     /gene="pyrD"
                     /locus_tag="POA77_001029"
     CDS             6918..7928
                     /gene="pyrD"
                     /locus_tag="POA77_001029"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI6364498.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8102..8644
                     /gene="zapC"
                     /locus_tag="POA77_001030"
     CDS             8102..8644
                     /gene="zapC"
                     /locus_tag="POA77_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDI6364499.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8641..9750)
                     /gene="ycbX"
                     /locus_tag="POA77_001031"
     CDS             complement(8641..9750)
                     /gene="ycbX"
                     /locus_tag="POA77_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDI6364500.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9994..12102
                     /gene="rlmKL"
                     /locus_tag="POA77_001032"
     CDS             9994..12102
                     /gene="rlmKL"
                     /locus_tag="POA77_001032"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDI6364501.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12114..14021
                     /locus_tag="POA77_001033"
     CDS             12114..14021
                     /locus_tag="POA77_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6364502.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14151..15404
                     /gene="pqiA"
                     /locus_tag="POA77_001034"
     CDS             14151..15404
                     /gene="pqiA"
                     /locus_tag="POA77_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDI6364503.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15409..17049
                     /gene="pqiB"
                     /locus_tag="POA77_001035"
     CDS             15409..17049
                     /gene="pqiB"
                     /locus_tag="POA77_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDI6364504.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17046..17609
                     /gene="pqiC"
                     /locus_tag="POA77_001036"
     CDS             17046..17609
                     /gene="pqiC"
                     /locus_tag="POA77_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDI6364505.1"
                     /translation="MKKCLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17865..18032
                     /gene="rmf"
                     /locus_tag="POA77_001037"
     CDS             17865..18032
                     /gene="rmf"
                     /locus_tag="POA77_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDI6364506.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18102..18620)
                     /gene="fabA"
                     /locus_tag="POA77_001038"
     CDS             complement(18102..18620)
                     /gene="fabA"
                     /locus_tag="POA77_001038"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDI6364507.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18689..20449)
                     /locus_tag="POA77_001039"
     CDS             complement(18689..20449)
                     /locus_tag="POA77_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDI6364508.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20635..21087
                     /gene="matP"
                     /locus_tag="POA77_001040"
     CDS             20635..21087
                     /gene="matP"
                     /locus_tag="POA77_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDI6364509.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21163..22203)
                     /gene="ompA"
                     /locus_tag="POA77_001041"
     CDS             complement(21163..22203)
                     /gene="ompA"
                     /locus_tag="POA77_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDI6364510.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22560..23069)
                     /gene="sulA"
                     /locus_tag="POA77_001042"
     CDS             complement(22560..23069)
                     /gene="sulA"
                     /locus_tag="POA77_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDI6364511.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23288..23917
                     /gene="sxy"
                     /locus_tag="POA77_001043"
     CDS             23288..23917
                     /gene="sxy"
                     /locus_tag="POA77_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDI6364512.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23880..26042)
                     /gene="yccS"
                     /locus_tag="POA77_001044"
     CDS             complement(23880..26042)
                     /gene="yccS"
                     /locus_tag="POA77_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDI6364513.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPIYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26052..26498)
                     /locus_tag="POA77_001045"
     CDS             complement(26052..26498)
                     /locus_tag="POA77_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDI6364514.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26621..28675
                     /gene="helD"
                     /locus_tag="POA77_001046"
     CDS             26621..28675
                     /gene="helD"
                     /locus_tag="POA77_001046"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDI6364515.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFHQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWCKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28707..29165)
                     /gene="mgsA"
                     /locus_tag="POA77_001047"
     CDS             complement(28707..29165)
                     /gene="mgsA"
                     /locus_tag="POA77_001047"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDI6364516.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29261..29923)
                     /locus_tag="POA77_001048"
     CDS             complement(29261..29923)
                     /locus_tag="POA77_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDI6364517.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPLLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30096..30509
                     /locus_tag="POA77_001049"
     CDS             30096..30509
                     /locus_tag="POA77_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDI6364518.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30554..30871)
                     /gene="hspQ"
                     /locus_tag="POA77_001050"
     CDS             complement(30554..30871)
                     /gene="hspQ"
                     /locus_tag="POA77_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDI6364519.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30929..32119)
                     /gene="rlmI"
                     /locus_tag="POA77_001051"
     CDS             complement(30929..32119)
                     /gene="rlmI"
                     /locus_tag="POA77_001051"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDI6364520.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32214..32492
                     /gene="yccX"
                     /locus_tag="POA77_001052"
     CDS             32214..32492
                     /gene="yccX"
                     /locus_tag="POA77_001052"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI6364521.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(32489..32818)
                     /gene="tusE"
                     /locus_tag="POA77_001053"
     CDS             complement(32489..32818)
                     /gene="tusE"
                     /locus_tag="POA77_001053"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDI6364522.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32909..33568)
                     /gene="yccA"
                     /locus_tag="POA77_001054"
     CDS             complement(32909..33568)
                     /gene="yccA"
                     /locus_tag="POA77_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDI6364523.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSVGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33775..33862)
                     /locus_tag="POA77_001055"
     tRNA            complement(33775..33862)
                     /locus_tag="POA77_001055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33826..33828),aa:Ser,seq:tga)
     gene            34289..35407
                     /gene="hyaA"
                     /locus_tag="POA77_001056"
     CDS             34289..35407
                     /gene="hyaA"
                     /locus_tag="POA77_001056"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDI6364524.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            35404..37197
                     /gene="hyaB"
                     /locus_tag="POA77_001057"
     CDS             35404..37197
                     /gene="hyaB"
                     /locus_tag="POA77_001057"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDI6364525.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            37216..37923
                     /gene="hyaC"
                     /locus_tag="POA77_001058"
     CDS             37216..37923
                     /gene="hyaC"
                     /locus_tag="POA77_001058"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDI6364526.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37920..38507
                     /locus_tag="POA77_001059"
     CDS             37920..38507
                     /locus_tag="POA77_001059"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDI6364527.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            38504..38902
                     /gene="hyaE"
                     /locus_tag="POA77_001060"
     CDS             38504..38902
                     /gene="hyaE"
                     /locus_tag="POA77_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDI6364528.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVESQQERAS"
     gene            38899..39756
                     /locus_tag="POA77_001061"
     CDS             38899..39756
                     /locus_tag="POA77_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDI6364529.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39890..41434
                     /gene="appC"
                     /locus_tag="POA77_001062"
     CDS             39890..41434
                     /gene="appC"
                     /locus_tag="POA77_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDI6364530.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            41446..42582
                     /gene="appB"
                     /locus_tag="POA77_001063"
     CDS             41446..42582
                     /gene="appB"
                     /locus_tag="POA77_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6364531.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            42595..42687
                     /gene="cbdX"
                     /locus_tag="POA77_001064"
     CDS             42595..42687
                     /gene="cbdX"
                     /locus_tag="POA77_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDI6364532.1"
                     /translation="MWYFLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42767..44065
                     /gene="appA"
                     /locus_tag="POA77_001065"
     CDS             42767..44065
                     /gene="appA"
                     /locus_tag="POA77_001065"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDI6364533.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(44180..46360)
                     /gene="etk"
                     /locus_tag="POA77_001066"
     CDS             complement(44180..46360)
                     /gene="etk"
                     /locus_tag="POA77_001066"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDI6364534.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKCTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(46380..46826)
                     /gene="etp"
                     /locus_tag="POA77_001067"
                     /gene_synonym="yccY"
     CDS             complement(46380..46826)
                     /gene="etp"
                     /locus_tag="POA77_001067"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDI6364535.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLDGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46814..47953)
                     /locus_tag="POA77_001068"
     CDS             complement(46814..47953)
                     /locus_tag="POA77_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDI6364536.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47999..50095)
                     /locus_tag="POA77_001069"
     CDS             complement(47999..50095)
                     /locus_tag="POA77_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDI6364537.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLSEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(50095..50841)
                     /locus_tag="POA77_001070"
     CDS             complement(50095..50841)
                     /locus_tag="POA77_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDI6364538.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50838..51482)
                     /locus_tag="POA77_001071"
     CDS             complement(50838..51482)
                     /locus_tag="POA77_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDI6364539.1"
                     /translation="MRPLILSIFALFLAGCTYSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(51589..51894)
                     /locus_tag="POA77_001072"
     CDS             complement(51589..51894)
                     /locus_tag="POA77_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HDI6364540.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        12922 a        12816 c        13704 g        12676 t
ORIGIN      
        1 tcaggatgat gacgatgaaa aaaagtgtat tgacggcgtt tataactgtg gtatgtgcaa
       61 cgtccagcgt tatggcagct gatgataatg ctatcacgga tggcaaagta acatttaatg
      121 gtaaagttat tgctccagct tgtaccctgg tagctgcgac gaaagattca gtagtcacat
      181 tgccaaatgt cagcgccacc aagttgcaaa ctaatggagc agtctctggc gttaaaactg
      241 atgtaccaat tgccttagaa ggctgtgatg ttactgtaac taaaaatgcg acgtttacct
      301 ttagtggtac ggctgacggc gtgcaaccta cagcatttgc caaccaggct accaccgatg
      361 cagcgacgaa tgttgctcta caaatgtatc tgccagatgg ttctacatcg gtaactcccg
      421 gtaccgaaac gtcgaatatt cagttagcgg attctgctga gcaaacagtg acctttaaag
      481 ttgattatat cgctacgggg aaagcgactt ctggtaatgt gaatgcggta acaaatttcc
      541 atattaacta ttattaatag aactcattaa ttgttttatt aattagtacc cctctagtgt
      601 tctggagggg atattcatat tttttaagag tgactattta tgaaaacttg cataacaaag
      661 ggaattgtga ccgtaagttt tacggcaata ttactcagct gctcatccgc atgggcggca
      721 ggtaaaggcg ggattggact tgcagcaaca cgtcttgttt attcagaagg tgaagagcaa
      781 atttcactgg gtgtacgtaa caccagtccg gatgttcctt atcttattca gtcatgggtg
      841 atgaccccag ataataaaaa atcagcagat tttattatta caccaccgct atttgtgctg
      901 aatccggcta atgagaatct gttacgcatt atgtacattg gtgcgccgtt ggcgaaagac
      961 agagaaaccc ttttcttcac tagcgtacgg gcagtccctt caacaacgaa gcgggaagag
     1021 ggaaataccc tgaagattgc cacacaaagc gtcatcaaac ttttctggcg accaaaaggt
     1081 ttagcgtatc ccttaggcga ggctccggcg aaactgcgtt gcacttcgtc agctgacatg
     1141 gttacggtca gtaacccaac accttatttc attaccctga cagacctgaa aataggtgga
     1201 aaagtagtta aaaatcaaat gatttccccc tttgataaat accaattttc tctgccaaag
     1261 ggggccaaaa atagcagcgt aacgtatcga accatcaatg actacggggc ggaaacgccg
     1321 caactcaact gtaaatcgta agccgtcttc agttaagaga gcgagatgta tagaactcat
     1381 cgacaacaca gcctgttaag ctctggtgga gtgccatcgt ttattggtgg gctggtggtg
     1441 tttgtgtcgg cagcgttcaa tgcacaagct gaaacctggt tcgatcctgc ttttttcaaa
     1501 gatgatccct caatggtggc cgatttgtct cgtttcgaaa aaggacaaaa aataacgcca
     1561 ggggtttatc gtgtcgatat tgttctgaat cagacaattg tagatacgcg caacgtcaat
     1621 tttgttgaga taacgccaga gaaggggatt gccgcctgtt tgacgactga aagcctggat
     1681 gcaatgggtg ttaatactga tgcgtttccg gcttttaaac aactggacaa acaagcgtgt
     1741 gtgccattgg cggagattat tccggatgcc agcgtaacgt ttaatgtgaa taaactccgt
     1801 ctggaaattt cagtaccgca aatcgccatc aaaagtaatg cccgtggtta tgtcccccct
     1861 gaacgttggg atgaagggat caacgcgcta ttactgggat attcgtttag cggggctaac
     1921 agtattcata gcagcgcaga cagtgattct ggcgacagct attttctgaa tttaaacagt
     1981 ggcgttaatt taggcccatg gagattgcgc aacaattcaa catggagtcg cagtagtggc
     2041 caaaccgcag aatggaagaa tctcagcagc tatttgcagc gggcggttat tccactaaaa
     2101 ggcgaactga ccgttggtga tgattatact gcaggcgatt ttttcgatag tgtcagcttt
     2161 cgtggtgtgc agctggcgtc agatgacaac atgctgccag acagcctgaa agggtttgcg
     2221 cctgtggtgc gtggtatcgc caaaagcaat gcccagataa cgattaagca aaatggttac
     2281 accatttacc aaacttatgt atcgcctggt gcttttgaaa ttagtgatct ctattccacg
     2341 tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg acggcagcgt caatagctac
     2401 agcgtaccgt tttccagcgt gccattactc cagcgtcagg ggcgaatcaa atacgcggtg
     2461 acactggcga aatacagaac caatagtaat gaacagcagg agagcaaatt tgcccaggcc
     2521 acgttgcagt ggggcggacc gtggggaacg acatggtatg gtggtggaca atatgctgaa
     2581 tattaccgtg ccgccatgtt tggtctggga tttaaccttg gcgatttcgg agcaatttcg
     2641 ttcgatgcga cccaggcgaa gagtacgctg gcagaccaaa gcgaacataa aggtcagtca
     2701 tatcgttttc tgtatgccaa aacgctcaac caattgggca ctaattttca attgatgggc
     2761 tatcgctatt cgacgtcggg tttctacacc ctttccgaca ccatgtataa acatatggat
     2821 ggctacgaat ttaatgacgg tgatgatgaa gatacgccga tgtggtcgcg ttattacaat
     2881 ttgttttaca ccaaacgtgg caaactgcag gtcaatatct cccagcaatt aggcgagtac
     2941 ggttcgtttt atttaagtgg tagccagcaa acttactggc ataccgatca acaggatcgg
     3001 ctattacagt ttggctacaa cacgcaaatt aaagatctct cgctgggggt ttcctggaac
     3061 tacagtaagt cccgtggtca acctgatgct gatcaggtgt ttgcactaaa tttttccctg
     3121 ccgctcaatc tgttgctccc cagaagtaat gatagctata ccaggaaaaa aaattacgcc
     3181 tggatgacct ctaacaccag tatcgataac gaagggcaca ttacacaaaa cctgggttta
     3241 acggagacac tactcgatga cggtaacctg agctacagcg tgcaacaggg atataacagc
     3301 gaggggaaaa cggctaatgg tagcgccagc atggactaca aaggggcgtt tgcagatgcc
     3361 cgagtgggct acaactacag cgataacggc agtcaacaac aactgaacta cgctctttca
     3421 ggcagtttag ttgcccattc acagggcatt accctggggc aatcgctggg ggaaactaac
     3481 gtgctgattg cagcaccagg cgcagagaat actcgtgtgg cgaacagcac cgggctgaaa
     3541 actgactggc gcggatatac cgttgtcccc tatgccactt cttatcggga aaatcgaatc
     3601 gcacttgatg cggcatcgtt aaaacgtaac gtggatcttg aaaatgcagt agtcaacgtg
     3661 gttcccacca aaggggcgtt ggttctggcg gagttcaatg cccatgcggg agcaagggta
     3721 ttaatgaaaa catcaaagca gggtataccg ctgcgttttg gcgcgatagc gacgctggac
     3781 ggcatacaga ctaatagcgg cattattgat gatgatggct cgctctatat gtccggtttg
     3841 cccgctcagg gagcgataac tgtacgctgg ggcgaagctc ccgatcaaat ttgtcatatc
     3901 agttaccagc ttaccgaaca acaaattaac tctgcgatta cgcgaatgga tgccatatgc
     3961 agataatctt tggagaaaaa tgcgtgttat tactacgact attttttgcc gccgtcttaa
     4021 tgctatggtg cgctcaaact gctgcttata gcgggcagtg tcataccact caggggaatc
     4081 cgtatattgg cgtcaatttt ggcgttaaaa ccctggagga agaagaaaat acggctggag
     4141 tagttaaaga caaattttat cagtggaacg aatcgaatga ttattatgtt tcctgtgatt
     4201 gcgataaaga caatgtcaga agtggccgat gggcattcgc cgcggattca ccgttagtct
     4261 atttaggcga caactggtac aaaattaatg actatcttgc cgccaaagtt ttattgcagg
     4321 ttaaaggcag ttctcctact gcggttcctt tcgaaaacgt gggcacaggg gcagatacac
     4381 gatggcatat ttgcgatccc ggcggtcaac gtttaggtgg ccagggggct agcggtaata
     4441 gcggtagctt ttccctgaaa atattgcagc cgttcgttgg ttcggtcgtc attcctccta
     4501 tggcgctggc gcgattattt gaatgctaca acatacccgc aggtgattcc tgcacgacta
     4561 caggtacatc ggttttagtg tattacctgt ctggtacgat caattcactt ggctcatgtt
     4621 ccgtcaatgc cggagagact atcgaggtcg atctgggcga cgtatttgcg gctaactttc
     4681 gtgttgtagg gcataaaccc cttggggcca gaacggcaga actcgcaatt ccagtcaggt
     4741 gtaacacggg aaacgcgggg ttagttaacg tcaacctgag tctgacggca accacagacc
     4801 ccagctatcc ccaggcgatt aagacgtcac gtcctggcgt gggcgtggtg gtgaccgata
     4861 gccagaacaa cattatttcc cctgctggtg gaacattacc gctctctatt cctgatgatg
     4921 cagacagtat cgcgcgaatg aatgtctatc cagtcagcac gacaggtgta ccaccagaaa
     4981 ccgggcgatt tgaagccacg gcaacggtga gaataaattt tgattaacgc gtgaacgtat
     5041 gaagaaaaaa acgatatttc agtgcgttat cttgttcttt agcattctta acatccatgt
     5101 cgggatggct gggcctgaac aagttagtat gcatatttat gggaatgtgg tcgatcaggg
     5161 ctgtgatgtc gccaccaaaa gtgcattaca aaatattcat attggtgatt ttaatatcag
     5221 tgattttcag gccgcgaata ccgtaagcac tgctgctgat ttgaatatcg atatcaccgg
     5281 ttgtgccgct ggtattactg gcgcggacgt cctttttagc ggcgaggctg acacccttgc
     5341 gccgacactg ctcaaactaa ctgacacagg cggaagcggt ggtatggcaa cggggattgc
     5401 cgtgcaaatt cttgatgcgc aaagtcagca agaaatcccg ctcaatcagg tccagcctct
     5461 tacgccctta aaagccgggg ataacacact caaatatcaa cttcgttata agtccacaaa
     5521 ggcgggagca acgggcggta atgcgacggc ggttctctat tttgatctgg tttaccagtg
     5581 aaggagtgat gttgaaacgg ataatctgga ttctgttctt actgggatta acggggggct
     5641 gtgagctatt tgcccatgat ggcacagtca acattagcgg atcgtttcgc cgtaatacat
     5701 gcgtgctggc acaggatagc aagcaaatca atgtgcagtt aggcgatgtc tcgctgacac
     5761 gtttttttca tgggaattat ggcccggaga aatctttcat cattaatttg caggattgcg
     5821 gaacagatgt cagcacggtt gacgtcacct tttcaggaac ccccgacgga gtgcagagcg
     5881 aaatgctgtc cattgaaagt ggtacggatg cagccagcgg gctggcaatt gcgattctgg
     5941 atgatgcgaa gatattgatt ccgcttaacc aggcgagtaa agactacagc ctacatagcg
     6001 gtaaggtgcc attaactttt tatgcgcaat tgcgacctgt caatagcgat gtgcagtcgg
     6061 gtaaagtgaa tgccagcgcg acatttgtac ttcattatga ctaatacgtg gaacagattg
     6121 gcgctcttga ttttcgccgt tttatcgctg ctggtggcgg gtgaattgcg ggcaggagtg
     6181 gtggtcggcg gaacgcgatt tatctttccg gcagacagag aatcgatatc tattttactg
     6241 actaatacca gtcaggaatc ctggcttatt aacagtaaaa tcaaccgccc aacgcgttgg
     6301 gccggggggg aagcgtcgac agtgccagca ccattactgg ccgctccgcc acttattctc
     6361 ctgaagcccg gtactacagg cacgttgcgc ttgctgagaa cggaaagcga catcttgcct
     6421 gtggatcgcg aaacgctatt tgagttaagc attgccagcg tgccatccgg caaagttgaa
     6481 aatcagagcg taaaagtggc gatgcgctcg gtatttaaac tgttctggcg acccgaaggt
     6541 ttgccgggcg acccgctgga agcttatcaa caattacgct ggacacggaa ttcgcagggt
     6601 gtacaactca ctaacccaac gccttactac attaacctga ttcagttgag cgtaaatggt
     6661 aaagcgttaa gtaatgcggg agttgtgccg cctaaaagcc agcgtcagac aagctggtgt
     6721 caggcaatcg caccgtgtca tgtcgcctgg cgggcgataa atgattacgg cgggttgagt
     6781 gcaaagaagg agcaaaatct gccctgaaac aggttcggaa aacgtttgcg ttttttttgc
     6841 cgcaggtcaa ttcccttttg gtccgaactc gcacataata cgcccccggt ttgcacaccg
     6901 ggaatccagg agagttcatg tactacccct tcgttcgtaa agcccttttc cagctcgatc
     6961 cagagcgcgc tcatgagttt acttttcagc aattacgccg tattacagga acgccgtttg
     7021 aagcactggt gcggcagaaa gtgcctgcga aacctgttaa ctgcatgggc ctgacgttta
     7081 aaaatccgct tggtctggca gccggtcttg ataaagacgg ggagtgcatt gacgcgttag
     7141 gcgcgatggg atttggatcg atcgagatcg gtaccgtcac gccacgtcca cagccaggta
     7201 atgacaagcc gcgtctcttt cgtctggtag atgccgaagg tttgatcaac cgtatgggct
     7261 ttaataatct tggcgttgat aacctcgtag agaacgtaaa aaaggcccat tatgacggcg
     7321 tcctgggtat taacatcggc aaaaataaag atacgccagt ggagcagggc aaagatgact
     7381 atctgatttg tatggaaaaa atctatgcct atgcgggata tatcgccatc aatatttcat
     7441 cgccgaatac cccaggatta cgcacgctgc aatatggtga agcgctggat gatctcttaa
     7501 ccgcgattaa aaataagcaa aatgatttgc aagcgatgca ccataaatat gtgccgatcg
     7561 cagtgaagat cgcgccggat ctttctgaag aagaattgat ccaggttgcc gatagtttag
     7621 ttcgccataa tattgatggc gttattgcaa ccaataccac actcgatcgt tctcttgttc
     7681 agggaatgaa aaattgcgat caaaccggtg gcttaagtgg tcgtccgctt cagttaaaaa
     7741 gcaccgaaat tattcgccgc ttgtcactgg aattaaacgg tcgcttaccg atcatcggtg
     7801 ttggcggcat cgactcggtt atcgctgcgc gtgaaaagat tgctgcgggt gcctcactgg
     7861 tgcaaattta ttctggtttt atttttaaag gtccgccgct gattaaagaa atcgttaccc
     7921 atatctaatt atttcttcga attcctcttt ataagataat cagggcttta ttttcagccc
     7981 tggttgtttt atattcatcg ctgttgctta tttagacatt ttgtactttt attgttgagg
     8041 ttattaagcg aagcgacaat ggattgtgtt gctgcggttt tatagggtag gggagaggca
     8101 gatgcgaatt aaaccagacg ataactggcg ttggtattac gatgaagagc acgatcgaat
     8161 gatgctcgat ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca agatgttgac
     8221 tccagatgcc ttttctcccg caggcttttg cgttgacgac gccgcgctct atttctcttt
     8281 tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa aaagccgaac tggtgctgaa
     8341 tgcactggta gcaattcgct atctcaaacc gcaaatgccg aaaagctggc attttgtttc
     8401 ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc tgcgtttggt tgagcgatac
     8461 tcacgagcag gtcaatttgc tggtcgttga atctggcgaa aatgccgcac tatgcctgct
     8521 ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat
     8581 catgaacgac aggctgaaac cgcaggttaa tgttgacagc ttcagcctcg aacaggctgt
     8641 ctaacgcgcc aacttaagtg cagtcttcgg tacacagcta cagcaaagaa tggtgccatc
     8701 atcgcccatt gctgattttt tcagcggcgt gacttcgcct tctaaaagct gaacacggca
     8761 acttccacaa atgcccgcgc ggcaagaata agggatacga attccctgat tttctaattg
     8821 ctccagcaac acctgttggt tatttccacg aaatgcctgt ccctgccagt caatatctac
     8881 atttgcgtcc ggttgttgcg tgatgttggc ggtatcatca gcggcagctg cgccgtaaat
     8941 ttttgccgga gccgttgcca gaatttccac ctcatcgcca acgcgaatca cgccgctatt
     9001 acgggcaatt aaattctgac caaaatcgac atcgccgtta tcctgggcag tgcggaaaga
     9061 ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc cctttttctg ggctgacggt
     9121 ggtgaaaata cagcggctac aaggtttaac cacatcaaac accacatcac caatgcgaat
     9181 cactttccag ctatcttctt cccaggctga cgcgccggaa accaccagat tggggcggaa
     9241 ttgctccatt tttacactgg ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt
     9301 agcaagaagg taaggatagc catcagcaaa tgacaggggt acagtgttgt ggcgtttcac
     9361 gcgccgggtc atttgtggcc ccacccagcg taattgcact tcgcgggaga aaaatccact
     9421 tagccatttg ttgatcgcgt ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc
     9481 ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac tgccatctgg
     9541 tgcggtgaga tgcaagccat catgcacagg tgaaggagta aaccgtacca tctggggaaa
     9601 ctggcgagcg gtaataaacg taccgtcagg ttccgtgatc ataaagatgc gatcgaaggc
     9661 cagaccactg acatcagcca gagcatgtgt aagaccaatg ccgcgcatcg atttaacagg
     9721 atgaataaaa agccgggtta atgtcaccac ggtgcggtcc tcaaatgaaa ataagccctc
     9781 aactttatga catgacgcgc ttattagcta taatgcgcaa caattttctt agcgcctgtc
     9841 ccattaggct attttatttg tcagtttggc cctgggttgt gctcgaaatc ctcacgtact
     9901 tgtgtacgct ccggtttctc cgcgctgtcc atgtccagac tgactgcaac aattacgcct
     9961 actgcggtag gctcttagag taaaagtgac gatatgaatt ctctgtttgc cagtacggcc
    10021 cgtgggctgg aagagctgtt aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag
    10081 gtggttcagg gtggggtcca tttcaagggc gacacacggc ttgtttacca gagcctgatg
    10141 tggagccgcc tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt ttacagcgat
    10201 ttagacctct atctcggtgt tcaggcgatc aactggacag agatgtttaa tcctggcgcg
    10261 accttcgctg tccacttcag tggtttgaat gacaccatcc gcaacagtca gtacggcgcg
    10321 atgaaagtga aagacgcgat cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat
    10381 gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc tgcataaaga aaccgccagt
    10441 atcgctcttg atctgagtgg tgatggttta catctgcgtg gctatcgcga tcgtgctggt
    10501 attgcgccga tcaaagaaac cctggcagcc gctattgtga tgcgttccgg ctggcagcca
    10561 ggaacaccgc tgctcgatcc gatgtgtggt tccggtacgt tgctgattga agcggcgatg
    10621 ctagcgaccg atcgcgcacc aggcttgcac cgtgggcgtt ggggctttag cggctgggtg
    10681 cagcatgatg aagctatctg gcaggaagtg aaagcggaag cgcaaactcg tgcccgtaaa
    10741 ggcctggctg agtatagctc tcatttttac ggttcggaca gcgacgcacg ggtgattcaa
    10801 cgtgcgcgca ctaacgcccg tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa
    10861 gatgtcgcgc aactgaccaa tccgctgccg aaagggccgt acggcacagt gttgagcaac
    10921 ccgccatacg gggaacgtct ggacagcgaa ccggcgctga ttgcgctgca tagcctgctg
    10981 ggtcggatca tgaaaaacca gtttggtggc tggaatctct ctttgtttag tgcctcgccg
    11041 gatctgctaa gctgcttgca gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg
    11101 ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca ctccagacag caaaccggcg
    11161 atggtagcgg aagactacgc caaccgcctg cgtaagaacc tcaaaaaatt cgagaagtgg
    11221 gctcgccagg aaggaattga atgttaccgc ctgtatgacg ccgatctacc ggaatataac
    11281 gttgccgttg accgttatgc cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact
    11341 attgatgctc acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac catttcggta
    11401 ctggggattg cgccaaacaa actggtgctg aaaacccgtg aacgccagaa gggcaaaaat
    11461 cagtaccaga aactgggcga gaagggcgag tttcttgaag ttaccgaata taacgctcac
    11521 ttgttggtga acctgacgga ttacctcgat accggtctgt ttctcgatca ccgtatcgcc
    11581 cgtcgtatgc tcggtcagat gagcaaaggc aaagatttcc tcaacctgtt ctcttatacc
    11641 ggcagcgcca ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac cgtggatatg
    11701 tcgcgtactt atctggagtg ggcagaacgc aacctgcgtt tgaatggcct gaccgggcgt
    11761 gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttc
    11821 gatctgatct ttatcgatcc gccaaccttc tctaactcaa aacgaatgga agatgcgttt
    11881 gatgttcagc gcgatcatct ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt
    11941 gggacgatca tgttctcgaa caataaacgt ggcttccgta tggatctcga cggcctggca
    12001 aaactgggac tgaaagcaca agaaattacg caaaaaacgc tctcccagga tttcgcccgt
    12061 aaccgccaga tccacaactg ctggctgatt accgcagcct gaaaggaata gtaatgtcat
    12121 taatcagtat gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc gataacgcag
    12181 aactgcatat cgaagataac gaacgtgttt gtctggtggg ccgcaacggc gcgggcaaat
    12241 cgacgttaat gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc attatttacg
    12301 agcaagattt gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt gagggtagcg
    12361 tttatgattt cgttgccgaa ggcattgaag aacaagcgga atatctgaaa cgctatcacg
    12421 atatttcgcg cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg
    12481 ttcaggaaca gctggatcac cacaacctgt ggcagctgga aaaccgcatc aacgaagtgc
    12541 tggcgcaact ggggttagat cctaacgttg cgctgtcgtc gctttccggc ggctggttgc
    12601 gtaaagcggc attaggacgc gcgctggtga gtaatccgcg cgtgctgttg cttgatgaac
    12661 cgacaaacca cctggatatt gaaaccatcg actggctgga agggtttttg aaaactttca
    12721 acgggacgat tattttcatc tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca
    12781 ttgttgatct cgatcgcggc aagctggtga cctatccagg gaattacgac cagtacctgc
    12841 tggaaaaaga agaagccctg cgcgtggaag aattacaaaa tgccgagttc gatcgcaaac
    12901 tggcgcagga agaggtgtgg atccgccagg ggatcaaagc acgccgtacc cgtaatgaag
    12961 gccgcgtacg cgccctgaaa gcgatgcgtc gcgagcgtgg tgaacgtcgc gaagtgatgg
    13021 gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg taagatcgtt ttcgaaatgg
    13081 aagacgtttg ctaccaggtt gacggtaagc aactggtgaa agatttttcg gcccaggttc
    13141 tacgtggcga caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc acgctgctga
    13201 aactgatgct cggtcagctt caagcggaca gcgggcgtat tcacgttggc accaaactgg
    13261 aagtggctta tttcgatcag caccgcgcgg aactggatcc cgataaaacg gtgatggata
    13321 accttgccga aggtaagcaa gaggtgatgg ttaacggcaa gccacgccac gtattgggct
    13381 atttgcagga ctttctgttc catccgaaac gggcgatgac gccggtacgt gcgctttctg
    13441 gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc aacttattga
    13501 ttcttgacga accgaccaac gatcttgatg tcgaaacgct ggaactgctg gaagaactga
    13561 tcgacagcta tcagggcacg gtattgctgg ttagccacga tcgtcagttt gtcgataaca
    13621 ccgttacaga atgttggatc ttcgaaggcg gcggtaaaat tggtcgttat gtcggcggtt
    13681 atcatgatgc ccgtggtcag caagagcagt atgtggcgct caaacagcct gcggtgaaaa
    13741 aaaccgaaga agccgccgcg gcaaaagcag aaactgtaaa acgcagcagt agcaaactaa
    13801 gctataaatt gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat ctggaggcga
    13861 agctggaagc cctacagacg caagtggcgg atgcttcctt cttcagtcag ccgcatgagc
    13921 agacgcaaaa agtgcttgct gatatggctg ctgcagagca ggagctggag caagcctttg
    13981 aacgctggga gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat agtcgattta
    14041 aacagagaca gctgacaacg gtaaaattgc ctgatgaact gggtttatca ggcatgaaaa
    14101 ccaacacttt gttagtcatc tgtaaaatag cgcatcatta aggagtacca atgtgcgaac
    14161 atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga catgctggtg gcgttaccgc
    14221 gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt
    14281 gggatgcccc tcggcagcgt cccaccgcct atgcgttggc tgcactgttc atgctgttgc
    14341 tgtccaactt gtttcctttt gtgaatatga acgttgcagg agttaccagt gaaattacat
    14401 tactggaaat tcccggcgtg cttttttctg aggactacgc cagcctcggc acctttttcc
    14461 tgttgtttgt gcaactggtt cccgcgtttt gtctgataac cattctgtta ctggtgaatc
    14521 gcgcggaatt accggtccgt ttaaaagagc aactggcacg ggtgcttttt caactcaaaa
    14581 cctggggaat ggcggagatt ttcctcgctg gtgtgctggt cagtttcgtt aaactgatgg
    14641 cttacggcag cattggggta ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc
    14701 aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg ggacgacatc gccccgatgc
    14761 cagaactgcg ccagccgcta aaaccaggcg tcacggggat acgtcagggg ctgcgttcgt
    14821 gctcctgttg tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt tgtagtacca
    14881 aagggtacgt tcggcgtaga aacagcctgc agtggacact cgcgctgctt gtaacgtcca
    14941 tcatgctgta tcttccggct aatattttgc ccatcatggt gacggattta ttaggctcga
    15001 agatgccgtc gacgattctc gctggggtca ttctgttatg gagcgaaggc tcttatcccg
    15061 tcgctgcggt gatctttctg gccagtatta tggtaccaac gttaaagatg atcgccatcg
    15121 cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga atgcatttga
    15181 tttatgaagt tgttgagttt gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg
    15241 tgctctcggc gctggtgcgt atgggaggtt taatgagtat ttatccggca atgggtgcat
    15301 taatgtttgc tttagtcgtc ataatgacaa tgttttctgc tatgacgttt gacccgcgtt
    15361 tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc ctgacagtat ggaatctaat
    15421 aatggggaag ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat atttcctatc
    15481 gtcacggcgc tcattggggc ctgggttctt ttttatcatt acagccatca gggaccggaa
    15541 gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac cattaaaagc
    15601 cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg ctgatgattt gacgcacgtt
    15661 gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga caccgtcttt
    15721 tgggtggtga aaccgcagat tggtcgcgaa gggattagcg gcctgggaac gctgctgtct
    15781 ggagtttata tcgaactgca gccaggcgcg aaaggcagca aaatggataa atacgatttg
    15841 ctggactcgc caccgttggc cccgcctgat gcgaaaggta tccgtgtgat tctcgatagc
    15901 aaaaaagccg ggcagctctc gccaggagat ccggtgctgt tccgtggcta tcgggtaggt
    15961 tcggttgaaa ccagcacctt cgatacacaa aaacgcaata tcagctatca actgttcatc
    16021 aatgcacctt atgaccgact ggtgaccaac aatgttcgct tctggaaaga tagtggcatt
    16081 gcggttgatc tgacgtcagc agggatgcgt gtggagatgg gctcattgac aacgctgctg
    16141 agtggcggtg tcagctttga tgtgccggaa ggtctggatt tagggcagcc agtggcaccg
    16201 aaaacagctt tcgttttgta tgatgatcag aagagcattc aggattcgtt gtacaccgat
    16261 cacattgatt atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc gggagctccg
    16321 gtagagttcc ggggtattcg cctgggtacc gtaagcaaag tgccattctt tgcgccgaat
    16381 atgcgtcaga catttaacga tgattaccgt attccggtac tgattcgtat cgagccagag
    16441 cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg
    16501 aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg tcactggtgc actgtatgtt
    16561 gatctcgatt tctatccaaa tacgcctgca ataaccggta ttcgtgaatt taatggttat
    16621 cagattatcc cgaccgttag cggcggcctg gcgcaaatcc agcaacgact gatggaagcg
    16681 ttggataaga tcaacaaact gccattgaat ccgatgattg aacaggcaac cagtacgctt
    16741 tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc tggatagcat gaacaagatc
    16801 ctcgctagcc agtcgatgca gcagttgccg acggatatgc agtcaacgtt gcgtgaattg
    16861 aatcgcagca tgcagggctt ccagcctggc tccgcagcct acaacaagat ggtggcggat
    16921 atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac gctcaatgag
    16981 aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag atccagagcc gaagagggcg
    17041 aaacaatgaa aaagtgtcta gtgacgattg cagcactgtg gctggtcgga tgcagctccg
    17101 gcgaaattaa taaaaactat taccagttac ctgtggtgca gagcggtaca caaagtaccg
    17161 ccagccaggg caatcgtctg ttatgggtag agcaggtcac tgttcctgac tatctggcgg
    17221 ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt gattgccaac aacaacttgt
    17281 gggccagccc gttggatcaa cagttgcgca acaccctggt tgccaacctg agtacgcaac
    17341 tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta
    17401 ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt
    17461 tgaaccacca gggacaactg atcaaacgtc cgttccgtct ggaaggagtg caaactcagg
    17521 atggttacga tgagatggtt aaagtactgg ccagtgtctg gagtcaggaa gccgcttcta
    17581 ttgcgcaaga gataaagcgt ctaccttaat tataaagatt tgtaaatata accgtctccg
    17641 gtatgttgcc tgaggcggtt tttttgtttc taacgtgcgg aaaaatttgt tcctcttcac
    17701 attttttgta caaccgacat gcccgtgtag ctcacaaata tgacagtggc gtgaattttg
    17761 cgcattgacg gcagttatga ttcgcggtat tgcttaactg tgattgcaca tttagtaatc
    17821 actgttttct tttccaccag aaaccagtat gagggaaacg aggcatgaag agacaaaaac
    17881 gagatcgcct ggaacgggca catcaacgtg gttatcaggc cggcatcgcc ggacgctcaa
    17941 aagaaatgtg tccctatcag acgctgaatc aaaggtcaca atggctggga ggctggcgag
    18001 aagccatggc ggacagggta gtaatggcct gattctgtct ctttaaaaag aaacctccgc
    18061 attgcggagg tttcgccttt tgatactctg tctgattata atcagaaggc agacgtatcc
    18121 tggaacagac cgactttcag gtcgctggcg gtatagataa gacgaccatc aaccagcact
    18181 tcgccatccg ccaggcccat aatcagacga cggttaacaa tgcgtttaaa gtgaatacgg
    18241 taggtcactt ttttcgctgt cggcagtacc tgaccagtga atttcacttc gccaacgccc
    18301 agcgcgcggc ctttaccttc gccgcccagc cagccgaggt agaaccctac cagctgccac
    18361 attgcgtcca ggcccaggca tcccggcata accggatcgc caataaagtg gcatccgaag
    18421 aaccacagat ccggattgat atccagttct gcttcaacat accctttgtc gaagttacca
    18481 cccgtttcgg tcattttgac cacacggtcc atcatcagca tgttcggtgc tggcaattgc
    18541 gggcctttag cgccaaacag ttcaccgcga ccagaggcaa gaaggtcttc ttttgtatag
    18601 gattcgcgtt tatctaccat gttctctgta agccttattt tattgaagca cgcaggatag
    18661 ctaacacgtg tacgctgaac aagtccgatc agttcggaat aaaccagttc agccaacgta
    18721 atggccatgg aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata cgttcctgga
    18781 tggtttgcat cagcgtcgtt tggccttcgc catcccacac cagatttaat aataacggca
    18841 gtgcgtcagt cacatcgtct actgcccaga tggtgaattt gccttcttct accgctttca
    18901 ccagttcact gtgaagactt aaatggcgaa cgttagctgt ggggataatg acaccttgtt
    18961 tcccggttaa ctcacgttgc tggcaaatag caaagaagcc ttcgattttc tcatttaaac
    19021 caccgaccgg ctgggcgcga ccgaactgat cgactgaacc tgtgatagcg atactctgat
    19081 tcaccggcac atcggcgagg gcgcttatca gggcgcagag ttcagccatc gaggcactat
    19141 ctccatcaac ttcactgtat gactgctcaa atgtcagcga tgctgagaag gggatctgtt
    19201 gctcaagctg tagttccgac atcaggaacg cttgcatgat catcatccct ttcgcatgga
    19261 tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca
    19321 caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga aattcaatga
    19381 ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct
    19441 gaaggatctc atcctgcata cgttcagcga ggaaaccttc gcgccattca cgctgctgca
    19501 gcattaagtt tagctgctcg ccggagaagg tgtcgccatc acacagggag gcgacctctt
    19561 tacactggcg gaggatccac tgcgggctaa gcggaagtgt ttcttgttca ccggtgtagc
    19621 gtgctgcttc gcggataagt accggccagg catccgctcc cggtgcaggt aagtgattat
    19681 gtctggcggt aaatgtcacc cagcgacacc actgggttac tgactctgca tcgacaatct
    19741 gcagagtatc ttcaaattcg ctataaatag cctgctctga aagctctggc tccatctcct
    19801 ggaaatcagc caatgattcg cgttcgccta ccagaatgac tttcagcttc aatggcatcg
    19861 aaggcacaga gacggggaga gggcgcgact catcaaacgc aacccagtca aaacgctcgc
    19921 ggttaacgat atttttcagc cgcatccaca gcagaggttg cgccagcagt gtacgcaaag
    19981 agatgatgag aataccgcca tttgcctgat gcaccagacc aggctgcagg gtaatgtcgc
    20041 cattaaactg gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg gcagcgacaa
    20101 cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag cggatggagt
    20161 ggccggaaac ctcatagtga ccgcccacca gttggcctgc atcgctttgt agcgtacgcg
    20221 cggcattggc aataagattc agatactcag actcttccgg ggccttcgcc agcataaaag
    20281 aggaggatgc tcgcgtatgc agcaactgct ccagtgcgaa ttgcagtcgc ggttgagtat
    20341 cactgaataa aggatcgttt tcgtcaatca aatgtggctg agcaaatatt tcctgatagc
    20401 tatcggtatc aggaaccagg tcacgccatg caagtttcgt aatggtcaaa gttgatgttt
    20461 tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc actacagcac acgtagaaag
    20521 caccgacaat actcctggca tgggcgttaa agctcacagg atggagattc ttttcttcac
    20581 tggcctaaaa agctgatatt ctgtaaagag ttacacggta acattgagat cgctatgaaa
    20641 tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga agtatctggt gaagaagcat
    20701 cgcgaagggg agttaatcac ccgttacata gaagccagtg ccgcccagga agccgtcgat
    20761 gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg gctggattga caagcatatg
    20821 aatccggaac tggtcaatcg catgaagcaa accattcggg caagacgaaa gcgccatttt
    20881 aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc tggaatttat tgtctggcaa
    20941 cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt ctgaaacgat tgttcagctg
    21001 attgaagatg cggaaaacaa agagaaatac gcgaataaaa tgtcttctct gaagcaggat
    21061 ctgcaggcat tgctgggtaa ggaataactg acgaaagtca gttcaattta ctaaaggcaa
    21121 aaaaaacccc gcagcagcgg ggtttttcta ccagacgaga acttaagctt gcggctgagt
    21181 tacaacgtct ttgatacctt taacttcgat ctctacgcga cgatccggag ccaggcagtc
    21241 gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg ccagtaaccg ggttggattc
    21301 gcccatacca cgtgcggaga tcttgtctgc cgggatacct ttggagatca ggtaatcaac
    21361 aacagactga gcacggcgct cggacagacc ctggttgtaa gcgtcagaac cgatgcggtc
    21421 ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc aggttgctca gctggctgta
    21481 cagctgatcc agagcagcct gaccttccgg tttcagggtt gctttgttga agttgaacag
    21541 aacgtcagac ttcagagtga agtgcttggt ctgtacttcc ggtgccggag ctggagccgg
    21601 agcaactact ggagctgctt cgccctgacc gaaacggtag gaaacaccca ggctcagcat
    21661 gccgttgtcc ggacgagtgc cgatggtgtg tgcgtcaccg atgttgttgg tccactggta
    21721 ttccagacgg gtagcgattt caggagtgat cgcgtactca acaccgccag cgaagaccgg
    21781 agaaacgccg gtgtcgtggt ttttaccata aacgttggat ttagtgtctg cacgccatac
    21841 cataccaccc agacgagtgt agatgtccag gtcgtcagtg attgggtaac ccagtttagc
    21901 ggtcagttga acgccctgag ctttgtatgc accgttttca acgctgcctt tgtacggcat
    21961 acgacctaac cagtcgtaac ccatttcaaa gccaacatac gggttaacct ggtaaccacc
    22021 aaaagcacca gcgcccagtt ggttttcatg ggtcgggcca ttgttgttga tgaaaccagt
    22081 gtcatggtac tgggaccagc ccagtttagc accagtgtac caggtgttat ctttcggagc
    22141 ggcctgcgct acggtagcga aaccagccag tgccactgca atcgcgatag ctgtcttttt
    22201 cattttttgc gcctcgttat catccaaaat acgccatgaa tatctccaac gagataacac
    22261 ggttaaatcc ttcaccgggg gatctgctca atattaactc taccgatatc ttcggcttat
    22321 gccgagcacc cctggcgatg taaagtctac aacgtagttg aaaacttaca agtgtgaact
    22381 ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt ttgtcagcga
    22441 caatttacag aagaaaatcg cggaaaccgc ttcagacaag cctccgcaag gaaaattagt
    22501 cacgactgaa agcattggct gggcgacaaa aaaagttcca ggattaatcc taaatttact
    22561 taatgataca aattagagtg aatttttagc ccggaaagtt gtctcgtggc gtgagaggat
    22621 gcgcttaccg gacgcataat aaaccccata gcgttacctt catttgccgc atcaacaagt
    22681 tcagcatgct cttcttcagt caaatcatct gccaaccaac cgatcaccac actgtaattg
    22741 cccgtgcgta aagcgcgaac cattgactcc acagtgtggc aaggggagag ctggctaatc
    22801 tgcattactt tcgttaaggg tagcccagat gcctgaaccc attcccgact cagtttttgt
    22861 tgcggtgtta accagagttg ccagcgcgat tgctgaccga gttgctgtaa caatggcaac
    22921 agtagaagtt gcgtcatcat gggctgatct tcgcgataga caacttcact gataagcccg
    22981 gctgtagtgt tttccgtaga gacacgcgca attttacttg ctgcggatga gaacgacgaa
    23041 gaacgatgtg catagcctga agtgtacata atcaatccag cccctgtgag ttactgtatg
    23101 gatgtacagt acatccagtg acaacaaaga tcaaccctat tttcggaaag agcctcgcaa
    23161 attttgtcgt tggtgacggg aaaacataaa ttaatcttgc cccttaagaa taagttgcct
    23221 attttcgtag ttaacggatc cgttaatgtg aatcattctt ttatgttatg attttaaaag
    23281 gaattttatg aaaagcctct cctataagcg gatctataaa tcacaagaat acctggcaac
    23341 gttgggcaca attgaatacc gatcattgtt tggcagttac agcctgaccg ttgacgacac
    23401 ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca
    23461 gtactgtgta aaacatccgc ctgtctggct gacatataaa aagtgtggcc gatccgttac
    23521 cctcaattac tatcgggttg atgaaagtct atggcgaaat caactgaagc tggtgcgtct
    23581 gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc acgcgcaata cccgggaaag
    23641 actgaaagat ttgcccaata tgtcttttca tctggaagcg attctcgggg aggtggggat
    23701 taaggatgta cgggcgttac gtatacttgg ggcaaaaatg tgttggttgc gactgcggca
    23761 gcaaaacagt ctggtgacag aaaagattct gtttatgctt gaaggtgcca ttatcggcat
    23821 tcatgaagct gcgctcccgg tggcacgccg ccaggagctt gcagaatggg ctgactctct
    23881 tacgccgaaa caggagtttc ctgcggaact tgagtaatct ggcgttgcag acgaccaatc
    23941 tcaggcagta atgcaatcaa taatccaact tgttgtacga ccagaggttc tttgctgtct
    24001 gcccgtggtt caagttgctg catccgctgt ttcaggctag ctaatgcctc attgacgcgt
    24061 tcttcatcag caggttgatg atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga
    24121 aacgccagaa tttcaggatt agttaactgc tcccggtgag caccgagggc tgagatatag
    24181 ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta
    24241 acgttcggtt cgctggacat atttgatacc accgacgcca gttcagcatc acggttgtgt
    24301 gcatcgcggc gggcaatacg atacgccaga cggttatcac gcccctgatg gtattgctcc
    24361 agtatggcat cgagataccg gcagttggcc tctgtggcgc gttcgagcat tcgcggcaga
    24421 ttgcgaaact gccagtcagg ccagatgtag ctcactgccg cccacgcaat ggcacaacca
    24481 atcagcgtat cgattacgcg aggtaacgct acttcaaaac cttcacccag caagttaaaa
    24541 cacagtagca ccaaaagtgt gatgaacatc gttgcatggg catattgcac gttacggaag
    24601 gcaaaaaaga gcacgccggt aataaccagt agcaccagct gcccttccag tgacggtaca
    24661 aaccacagca caggaatgcc aatggcgata ccgaccagcg taccaataat ccttaacttc
    24721 aggcggtggc gcgtggcgtt atagtttggc tggcagacaa acaaacttgt cagcaagatc
    24781 caatacccgt gatgcattcc ggttatttga atgatggcgt agccgaagca caacaccagc
    24841 gacattctta ccgcatgacg gaagagggcg gattccggcg tgaagtgacg gctaagacgc
    24901 agccagatat cactcaaccc gtgcgggctg tcatcagcga gctcattttc gtcattatta
    24961 tggggtagtg cctgggcctg ttctgattca attgttgcca gttgggcatc aatggcgcgt
    25021 aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg cgggtgcgcc gttatcgcgc
    25081 atccgctcca gcgcagcatc aatatgcgta aaagcgcgct caaaatgcgg atcatgttga
    25141 taaggctgac gcagcaaaat acagcgtgac agttgctggc acgcctggcc ctgcatcgac
    25201 atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa aatgttcacg caatgtttga
    25261 tactgaatat gagaagagct ggcacgctcg tgaatatcct gcgcgacaaa gtaataatgc
    25321 agcgtgcgac gcgttccccg ttgaccacga tcgccacgta agcgggtcag cagcgagagt
    25381 ttcgtctgat tcaatgtcgc catcagttgt ccgttggcga gagccaaatc gtacagcggt
    25441 gcctggcttt catcttcaat atcaggatca aacatgcgcg acttgagctc aagataacgc
    25501 gccagttgtt catagcaacg cgccaggttg tcctgcagcg ggcggaccgg gaacagcaga
    25561 tgaccaataa gtgtcaggac gttgtaccag acggcaccgg ccagcagata aatcggctgc
    25621 tgataccagt gctcataaag tgatgttccc aacatagtgt aaatggcgat cagcaatgca
    25681 ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga gcaaaatgaa gccgctggta
    25741 gagagcgtta agccaatcgc aaatagccag ggccagggaa acagcaattc tactgaggcc
    25801 gaggcgataa aaaagcagaa cagcgtaatg atgaggttac gcaaacgtcc cgccagtcgg
    25861 tcatcgagat cggtcagcgc cgctgccacc atccccagcg ttagcggaat cgtcagtttt
    25921 acatcaccca gccaccacgg aaacgctgtg gttccacaaa gcgcaataaa aatacgcgcg
    25981 taatacagcc aggcgctgtt ccaggtatag cgtttgagca aaggacttag cataaaggcc
    26041 atattagtgg attattcaaa acgacgacgt gcattggctt cacgcgcagc ttgcgctgtt
    26101 tctaccgata ccacgcgacg accaaccggc catagtgcaa tagcggcaat tttaaagttc
    26161 gcaatgccga caggaatgcc aatgattgaa atacattgtg cgatgcccgt tgcaatgtgc
    26221 atcaggcata accaccagcc aaagaaaatc agccagaaaa tattcaatac cgtaccgcca
    26281 gtattcagca gcacattttt gccagccggg ttcagttcat cgacatgaat agcttcattg
    26341 ccataaggca ccagagacag tttagtgatc tcccagcagg atcgtgtcag cggtaaggta
    26401 aaaatcagca caatactgac cagagtcgcc aacagccagc ccagagtggt ggcaaatccg
    26461 ccaagcacaa agttcagaat gttcaaaacg gtacgcataa aacctcgctt tactgtggtt
    26521 ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg agcacgtttt ctctgacggt
    26581 tacactgata agaaataatt tcgtgtggat ctacagagtc atggaactga aagcgacaac
    26641 gcttggaaaa cgtctggcac agcaccctta cgatcgggcg gtgatcctca atgccgggat
    26701 taaagtctcc ggcgatcgcc acgaatacct tattcctttc aatcaattac tggcgattca
    26761 ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta ctgccggacg aaaaagtggt
    26821 gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt taccatcatc ttgatgctca
    26881 ctggcagcgg tggagtggcg agatgagcga aattgcgtct ggtgttttac gccagcaact
    26941 ggatttgatt gccacgcgca ctggagaaaa taaatggctg acgcgtgagc aaacctctgg
    27001 cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg ttgccggtta accgactgga
    27061 agaattcgat aactgccgtg aggcgtggcg taaatgtcag gcctggttga aagatattga
    27121 aagcgctcgg ttgcagcata accaggcgta taccgaagcc atgcttaccg agtatgcgga
    27181 ttttttccac caggtcgagt cttcaccgct gaatccggcg caggcccggg cagtcgttaa
    27241 tggcgagcat tctctgttag tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt
    27301 ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct
    27361 ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgt attcgcgaac ggctacatac
    27421 cgaagacatt accgcacgca cgtttcatgc gctggcgctg catattattc agcagggcag
    27481 caaaaaagtt ccgatagtca gcaaactgga aaatgatacc gctgcccgtc atgaactctt
    27541 tattgctgag tggtgcaagc aatgcagcga aaagaaagcg caggcgaagg gctggcggca
    27601 atggctgacg gaagaaatgc agtggtcagt gccagaaggt aacttctggg atgatgaaaa
    27661 attacagcgt cgtcttgcct ctcgcctcga tcgctgggta agtctgatgc ggatgcacgg
    27721 tggtgcacag gcagaaatga ttgccagtgc acccgaagag attcgcgatc tgttcagtaa
    27781 acgtatcaag ttgatggccc cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa
    27841 cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg attctggaga aaggtcgctt
    27901 tatcagcccg tggaagcata ttctggttga tgaatttcag gatatctcac cgcagcgggc
    27961 agcgttgtta gcggcattac gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga
    28021 tgactggcag gcgatttacc gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca
    28081 tgaaaacttt ggtgaaggcg atcgctgtga tttagacacg acttaccgtt ttaacagtcg
    28141 tatcggtgag gtggcaaacc ggtttattca gcagaaccca ggccagttga aaaagccgct
    28201 aaacagctta accaatggag acgaaaaagc cgtcacgtta ttggatgaga gtcaacttga
    28261 cgctttgctg gataagctct ctggttatgc caaaccggaa gagcgcattc ttatcctggc
    28321 gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt
    28381 gcaaatcgac tttatgacca ttcatgccag caaagggcaa caggcggatt acgtcatcat
    28441 cgttggcttg caggagggaa gtgatggttt tccggctgcg gcgcgggagt cgattatgga
    28501 agaggcgcta ctgccaccgg ttgaggattt cccggacgct gaagaacggc ggttaatgta
    28561 cgtggcgctg acccgggcac gccatcgggt atgggcactg tttaacaaag agaatccctc
    28621 tccctttgtg gaaatactga aaaatctgga tgtgccggtg gcgagaaaac cgtaagaaac
    28681 aggtggcgtt tgccacctgt gcaatattac ttcagacggt ccgcgagata acgctgataa
    28741 tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg actggattat gaagtctgcc
    28801 gttgccacgt tggtggcgac cggaatgttc cataccgtcg ccagacgcag caaggctttc
    28861 acgtcaggat cgtgcggcac ggcatttagt ggatcccaga agaaaatcaa tacatcaatt
    28921 ttcccttctg agatcaatgc gccaacctgc tggtcacccc ccattgggcc actcaacatc
    28981 gcgttgacgt tcatgccggt cgcgcgggaa attaagttac cggtagtgcc tgttgcatac
    29041 agtacgtgtt gttccagtaa cggttgatgc cgttccaccc agctcatcag catttgtttg
    29101 cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt
    29161 tccataatgt acatccgtag ttaactttcc tacagattac tgtaagcact tatcgctgca
    29221 agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt
    29281 ccgcccactg caggaaagta tttcgcgttt gtgggtcagc cagtttaaac caatacttca
    29341 gccgttgttc tgtgagcacc tgagactgcg gtggaatagc atccagctcg gaaacacccg
    29401 agagcagcgt actgtcatct gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg
    29461 cggatttgtt atagcgttca acctcgacct cataatcaat cgtttttgcc gttgaggtaa
    29521 tggcgagaat atccagcttt accggaatcg gcgtcgcatc gccatccaac aattcaagga
    29581 gcggcgcggc atcaaaatgg ttagcttccc gctcattctc caggcgaggc aggcgaaaat
    29641 tgacctggtt gatgagctgg gtattaaaac tgaccaccag cggtggggag atatacagcc
    29701 gttcttcact attggaaaga tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc
    29761 cattatccag ttcaatgctg tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt
    29821 cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg
    29881 gcaaacataa tgcaatcaag gtggtcacga tgccggtttt cataggtagc tcctgtcaaa
    29941 agaccgcccg aataataatg tgtcaaaatt tttctttaat catacttact aacatataga
    30001 catatttccc gcttttgctc tcattcattc gtattagctg catggttggc atgtcggctt
    30061 tgtcgtacac tttgttaaac agtctggagg aaataatgaa agaaaccgat attgcaggca
    30121 ttttaacgtc cactcacacc attgcgctgg tgggggcaag cgacaaaccc gatcgtccca
    30181 gctatcgcgt gatgaaatat ctactcgacc aggggtatca cgtcattccg gtttcgccaa
    30241 aagttgccgg caaaacgctg ctgggccagc aggggtatgg cacgctggca gacgttccgg
    30301 aaaaggtcga tatggtcgat gttttccgta attcagaagc ggcgtggggc gtggcacagg
    30361 aagctattgc cattggcgcg aaaaccctct ggatgcaact gggggtgatt aatgagcaag
    30421 cggcagtact ggcacgggat gccgggttaa atgtggtgat ggatcgctgc ccggctattg
    30481 agatccctcg cctgggcctg gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg
    30541 attcgttcag ggattagtta cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg
    30601 ccagctcatc catggatggc tgttcaggat gttcatcctg caattcgcta cttagctggg
    30661 cttcagccag gtaggtatga accggtaggc cgttatcgtc ctccatcacc acgtgatacc
    30721 acggagcggc gcgaagctcg tcattcaccg ccagctcatc aggcgacggt tcagagagcg
    30781 aataaaccgg gtcgatatcc acgaccactc cgagataacc taacagggaa tggcggacct
    30841 gctggccgat accgaatttg ctggcaatca tagtcacctc ccgggaaatc tttacctttg
    30901 atataagggt aaaattccac atttcaagtt acatgacgcg acaggcaaac cctttcagat
    30961 atagcccttc cgggtaggta gcgatcaccg gatgatcggc tgcctgacgg aactgctcta
    31021 taaattgtac atcacggccg gcatcaattg cggcatccgc gatgattttc tgaaataaat
    31081 cgctggtcat cagaccggaa caggagaaag tcaggagaat accgccttca ttcagcaact
    31141 gaatcgccag catgttgata tctttatagc cacgacacgc gcccatcaac tggcttttat
    31201 tctcaacaaa cttcggcggg tccatcacga taacgtcaaa tttttcaccg cgatcgcgat
    31261 aagtacgcag caatttaaag acatcatcac ggacaaactc agccttgctc agatccagtt
    31321 tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc ctgggaggta tcaacgctga
    31381 caacctggct gcaaccgccc atcagtgccg atacggcgaa accaccggta taggagaaac
    31441 agttcagcac acgtttattt tcaacgtagc ggcgggtagc caggcggcta tcacgctggt
    31501 ccaggtagta gcccgttttg tgtccgtgct gaatatccac cagcagtttc atgccgtgtt
    31561 cttcaatcgg cagcagggca ggtggcaact cgccggtgac gaggccctgg gtcagctcca
    31621 tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta aatcgcacat tccgggtaca
    31681 gcgtttgcag ggcactaatt aatgccgcgc gctgatattc tgcgccagca ctcagcagtt
    31741 gcagcaccag aaaattaccg aaacgatcga tagtaatacc cggcaggcca tcagattctc
    31801 cggcgattaa acgatagctg tcgaggccat ctttttgcgc cagccagtca cgccattttt
    31861 gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat agactcagac gggtcaaacg
    31921 tccagacgcg cgcccggatt tgcgaagctg gtgaataagc gccgcgtgct aaccattttc
    31981 cctgatgatc aacaatatcg atggtttcac cgaggctggc tttaccttcc atgcgggcaa
    32041 cggccccgga aaagacccac ggatggcgac gaagtaatga tttttcgcgc cctttggcta
    32101 acactaaacg tacactcata atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt
    32161 ccggagtttt ccatggattt gcaacgcgtc cattaaggat aaggagagca gcaatgtcga
    32221 aagtctgcat aattgcctgg gtttacgggc gagttcaggg cgtaggattt cgctacacca
    32281 cacagtatga agcgaaaaga ctgggtttaa ccgggtacgc caaaaatctt gacgatggca
    32341 gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga aaaattaatg cagtggttaa
    32401 agagtggcgg cccgcgttct gcgcgtgtgg aacgggtgct tagcgagccg catcatccct
    32461 cgggggaatt agccgatttt cgtatccgtt aaatacattt taccggtttt ggcaggccag
    32521 caattttggt ggcttgctta gccggacctt tcggaaacag tcgatataaa tagcggctgt
    32581 tacctttttc ttcgccaaat ttattcgcca tcgcttttac cagcatacga atcgccggag
    32641 aagtattgaa ttccagatag aaatcacgca caaaacgcac cacttcccag tgttctggcg
    32701 acagcgaaat cccttcgttc tctgcaatca ccacagccag cggctcactc cactggctgc
    32761 tttctttgag atagccttcg gtatccgttt ctatctcttt accttcgaag atcagcataa
    32821 ttattactac cttaatcaga ctggcggcag tgtaacaaaa aacaaagccc cgcataaagc
    32881 gaggctatga aagtgttagc gggtgagatt aatcgcggct agcgaagccc agaatgctca
    32941 gcaggctgac gaagatgttg tacagcgaaa catacaggct aaccgtggca cgaatatagt
    33001 tcgtctcacc gccatgaatg atgttgctgg tttcaaacaa gatagcgcca gaggagatca
    33061 gaatgaagac cgcgctgatc gccagatgca gagcaggcag ttgcaggaag atattagcaa
    33121 ccataccaat cagcaccacc acaatacccg ccatcagcat accgccgagg aacgacatat
    33181 ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa cactaacgcc gttccgccca
    33241 gtgccatagc gattacgtca cccattccga cagacagata ggtgttcaga ataggtccga
    33301 ggatataacc cagaaaaccg gtaaaggcga atgcggagat aatcccggtc ggcttattcg
    33361 ccgttttata ggtcaggaac atcaaaccat acatacccac cagcgtcaga atcagacccg
    33421 gagatggcag catcagcacc gtactggcag ttgcggtaat cgccgaaaag gccagcgtca
    33481 ggctcagcag aaaataggta ttacgcagca ccttatgggt gctaagcagt gatgtacggt
    33541 catgtgaaga actaacaata cgatccattg agtcactctc tatgacagat gtaattaatt
    33601 aagcagcata atgataatgc gtaagggcac ccagaagttt tacccatctt tacgcatttg
    33661 atctggaaca ggtttaacag cggattatca ggtcattaag caaatataac gccctgagaa
    33721 tttcgacagg caaaagaaaa aggggttagc atttagctaa ccccttatct tatttggcgg
    33781 aagcgcagag attcgaactc tggaaccctt tcgggtcgcc ggttttcaag accggtgcct
    33841 tcaaccgctc ggccacactt ccggaatgac gcgcactata aacatcccga tgcggcgtgt
    33901 aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa gtgcattgat agcgtgaaat
    33961 aacagcagat tgatcatttc atcaccatga attccttctc ttttactcgt ttagcaaccg
    34021 gctaaacatc cccaccgccc ggccaaaaga aaaataggtc catttttatc gctaaaagat
    34081 aaatccacac agtttgtatt gttttgtgca aaagtttcac tacgctttat taacaatact
    34141 ttctggcgac gtgtgccagt gcagaaggat gagctttcgt tttcagcatc tcacgtgaag
    34201 cgatggtttg ccttgctaca gggacgtcgc ttgccgacca tgtgcgcccg gtgtcctgcc
    34261 ggtgtcgcaa ggagaagaga cgtgcgatat gaataacgag gaaacatttt accaggccat
    34321 gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat tgtagtctgg ctgccacgtc
    34381 gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg gcgctggaga acaaaccccg
    34441 cattccggtg gtatggatcc acggtctgga atgcacctgc tgtaccgaat cttttatccg
    34501 ctccgctcac ccactggcga aggacgtcat cctttccctg atttccctcg attacgacga
    34561 tactttgatg gctgccgccg gaacccaggc ggaagaagtc tttgaagaca tcatcgcgca
    34621 atacaatggc aaatatatcc tcgcagtaga aggtaatccg ccgctgggcg agcaggggat
    34681 gttctgtatc agcagcggtc gaccgtttat tgagaaactc aaacgtgccg ctgccggagc
    34741 cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc tgcgtgcagg ccgcgcggcc
    34801 caatccgacg caggcaacgc ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt
    34861 acctggctgc ccgccgatcc cggatgtgat gagcgccatc attacttaca tggtgacctt
    34921 tgatcgcttg ccagatgtcg acagaatggg ccgtccattg atgttctatg gtcagcgaat
    34981 ccacgataaa tgctatcgcc gcgcccactt cgacgccgga gagttcgtcc agagttggga
    35041 tgatgacgct gcccgcaaag gttactgcct gtacaaaatg ggttgcaaag ggccaaccac
    35101 ctataacgcc tgttcctcca cccgctggaa tgatggcgtt tctttcccaa tccagtctgg
    35161 tcacggctgc ctgggctgtg cggaaaatgg tttctgggat cgaggttcgt tctacagccg
    35221 cgtggtcgat attccgcaaa tgggtaccca ttccaccgcc gataccgtcg gtttaaccgc
    35281 acttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc gccagcgccg ttgaccagcg
    35341 cagacgccat aaccagcaac ctacagaaac cgaacatcag ccaggcaatg aggataaaca
    35401 ggcatgagca ctcagtacga aactcaggga tacaccatca ataatgccgg acgccgcctg
    35461 gtggttgacc cgattacgcg catcgaaggc cacatgcgct gcgaagtgaa tattaacgat
    35521 cagaatgtga tcaccaatgc cgtttcctgc ggcaccatgt ttcgcgggct ggagatcatc
    35581 ctgcaagggc gcgacccgcg cgatgcgtgg gcgttcgttg aacgtatctg cggcgtctgt
    35641 actggcgtac atgccctggc ttcagtttac gccatcgaag atgccatcgg tattaaagtg
    35701 ccggacaacg ccaatatcat ccgtaacatt atgctggcaa cgctctggtg ccacgatcac
    35761 ctggtgcact tctatcagct tgccgggatg gactggatcg atgtgttaga tgcgctgaaa
    35821 gccgacccgc ggaaaacctc cgaactggcg caaagtctct cctcatggcc gaaatcatcc
    35881 cctggctatt tcttcgacgt acaaaatcgc ctgaagaaat tcgttgaagg cgggcagttg
    35941 gggatcttcc gcaatggcta ctgggggcac ccgcagtaca aactgccgcc agaagctaac
    36001 ctgatgggct ttgcccacta cctcgaagct ctcgatttcc agcgtgaaat tgtgaaaatc
    36061 cacgcggtct ttggcggtaa aaacccgcat ccaaactgga ttgtcggcgg gatgccttgc
    36121 gccattaaca ttgacgaaag cggcgcggtc ggggcggtca atatggaacg cctgaacctg
    36181 gtgcagtcga ttatcacccg cacggcagac ttcattaaca acgtgatgat ccccgacgcc
    36241 ttagccatcg gtcagttcaa caaaccgtgg agcgaaatcg gcacgggtct ttctgataaa
    36301 tgtgtcctca gctacggcgc attcccggat attgccaacg actttggtga gaaaagtctg
    36361 ctgatgcctg gcggcgcggt gattaacggc gacttcaaca atgtgctgcc agtggatttg
    36421 gttgatccgc agcaggtgca ggagtttgtc gatcacgcct ggtatcgtta tcccaacgat
    36481 caggtcgggc gtcatccatt cgatggcatt accgacccgt ggtacaaccc cggcgatgtc
    36541 aaaggcagcg ataccaacat tcagcagctg aatgaacagg aacgctactc gtggatcaaa
    36601 gcgccacgct ggcgcggtaa cgcgatggaa gtggggccgc tggcacgcac gttaatcgct
    36661 tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc gcatgatgtc ggcgttgaac
    36721 ctgccgcttt ccggtatcca gtcaacgtta ggccgcattt tgtgccgcgc gcacgaagcg
    36781 cagtgggccg caggtaagtt gcagtatttc ttcgacaagc tgatgaccaa cctgaaaaac
    36841 ggcaatctcg ccactgcttc cacggaaaaa tgggaacctg caacctggcc gacagagtgc
    36901 cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag gccactgggc cgccattcgc
    36961 gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca cctggaacgc cagcccgcgc
    37021 gatcccaaag ggcagattgg cgcttatgaa gcggcgctga tgaacaccaa aatggcgatc
    37081 cccgagcaac cgctggagat cctgcgtact ctgcacagct ttgacccgtg cctcgcctgt
    37141 tcaacacacg tgctgggcga cgacggtagc gagctgatct ccgtgcaggt gcgttaacag
    37201 cgaaggagaa tcatcatgca acagaaaagc gacaacgttg tcagccacta tgtctttgaa
    37261 gcgccagtcc gcatctggca ctggttgacg gtgttatgca tggcggtgtt gatggtcacc
    37321 ggatacttta tcggcaagcc gctaccttcc gtcagcggcg aggcgacgta tctgttctat
    37381 atgggctaca tcaggttaat tcacttcagc gccgggatgg tttttaccgt ggttttgctg
    37441 atgcggatct actgggcttt tgttggcaat cgatactccc gcgagctgtt tatcgtgccg
    37501 gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa tccgctggta tctgtttctg
    37561 gcaaaacgtc cgagtgccga tataggccat aatcccatcg cccaggcggc gatgttcggc
    37621 tatttcctga tgtcggtctt tatgatcatc actggttttg cgctgtacag cgaacacagc
    37681 cagtacgcta tttttgcgcc gttccgttat gtggtggaat ttttctactg gacgggtggc
    37741 aactcaatgg acattcacag ctggcaccgg ctggggatgt ggctgattgg cgcgtttgtg
    37801 atcggtcatg tctacatggc actgcgtgaa gacatcatgt ccgacgacac ggtgatctcc
    37861 accatggtca acggctaccg tagccacaaa tttggcaaaa taagtaacaa ggagcgttca
    37921 tgagcgagca acgcgtggtg gtcatggggc tgggcaacct gctgtgggcc gatgaaggct
    37981 tcggcgtgcg ggtggcggaa cggctgtatg cccattacca ctggcccgag tatgtggaga
    38041 ttgtcgatgg cggtactcag ggactgaact tgctggggta tgtcgaaagc gccagccatc
    38101 tgttgattct cgatgccatt gactacgggc tggaacctgg aacgctgcga acctatgccg
    38161 gagaacgcat tccggcttat ctcagcgcga agaaaatgag cctgcatcag aacagtttct
    38221 ccgaagtgtt ggcgctggcg gatatccgcg gacatctgcc agcacatatt gccctcgtcg
    38281 gtctgcaacc cgcaatgctc gacgactacg gcggtagcct gagcgaactg gcacgggagc
    38341 aactgcccgc tgcggaacag gcggcgctgg cgcagctggc tgcgtgggga attgtgccgc
    38401 aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct gtcgatggaa aattacgaag
    38461 gcgttcgctt gcgccagtac cggatgacac aggaggagca gggatgagca acgacacgcc
    38521 atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg acgccagtca gtgaatcccg
    38581 tcttgacgac tggcttacgc aagcgccaga cggcgtggtg ttattaagca gtgacccgaa
    38641 acgcacgcca gaggtcagcg ataatccggt aatgattggc gaattactgc gcgagtttcc
    38701 cgactataca tggcaggtgg cgattgctga ccttgagcag agcgaagcca tcggcgatcg
    38761 ctttggcgtc tttcgctttc ctgccacttt agtgtttacc ggcggaaact atcgcggcgt
    38821 gctgaatggt attcacccgt gggcggaact gataaacctg atgcgcgggc ttgtcgaatc
    38881 gcagcaggag cgtgcctcat gagcgaaact tttttccatc tgctggggcc aggaacgcaa
    38941 ccgaacgatg acagtttcag catgaatcca ctgccgatca cctgtcaggt gaatgatgaa
    39001 ccgagtatgg cggccctgga gcaatgtgct cacagcccgc aggtgattgc gctgttaaac
    39061 gagttacaac atcaactaag cgaacgccaa ccgccgttgg gcgaggtgct ggcagtcgat
    39121 ctgttaaatc tcaacgccga cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa
    39181 gtgtcagtgc gcattcagca ggctgacgac agtgaaagtg aaatacagga ggcgatcttc
    39241 tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt tgctggagga caaactggag
    39301 gctggctgcg cgccgctggc gttgtggcag gcggcaacgc aaaatctctt gccgacagat
    39361 tcgctgttac cgccgcccat tgatggcctg atgaatggcc taccgttggc gcatgagtta
    39421 ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca ttaatctgac gcaattaccc
    39481 atcagcgagg ctgatcggct ttttctctca cgtctctgtg ggccgggaaa tattcagatt
    39541 cgtaccattg gctatggcga gagctatatc aacgccacgg ggttacgcca tgtctggcat
    39601 ttacgctgta cggacacctt aaaaggcccg ttactggaaa gttatgaaat ctgcccaata
    39661 ccggaagtgg tgctggcagc gccagaagat ttggtcgact ctgcgcagcg gcttagcgag
    39721 gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag acggtaagta tcgctttcag
    39781 tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag agtttcagga taaagaggga
    39841 gatctaccat tatcgggtta tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt
    39901 cattgattta tcgcgctggc agtttgctct gaccgcgctg tatcactttt tatttgtacc
    39961 ccttaccctg gggctgattt ttttgctggc tatcatggaa accatttacg tggtcaccgg
    40021 caaaacaatc taccgcgata tgacgcgctt ctggggtaag ctcttcggta tcaattttgc
    40081 tcttggcgtg gctaccggcc tgaccatgga gtttcagttt ggtactaact ggtcattcta
    40141 ttccaactat gtgggcgata tttttggcgc accactggcg atggaagcat taatggcctt
    40201 cttcctcgaa tccacctttg tcgggctgtt cttcttcggc tggcagcggc tgaataaata
    40261 ccagcacctg ctggtgacgt ggctggtggc gttcggttca aatctctctg cattgtggat
    40321 attgaatgcc aacggttgga tgcaataccc gaccggtgcg cattttgata tcgacaccct
    40381 gcgtatggag atgaccagct tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt
    40441 tgtgcacacc gtaatggcgg gctacgtgac cggggccatg tttattatgg cgatcagcgc
    40501 ctggtattta ctgcgcggac gggagcgcga tgtcgcatta cgttcgtttg ccatcggttc
    40561 cgtcttcggt actctggcga ttatcggtac cctgcaactc ggagacagtt ctgcgtatga
    40621 agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa ggggagtggc aaacggaacc
    40681 tgcacctgca ccgttccatg tggttgcctg gccggaacag gatcaagagc gtaacgcctt
    40741 tgccatcaaa attcccgcgc tgctagggat cctcgccact cactcattag ataaacccgt
    40801 gccgggtctg aagaatttga tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc
    40861 ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag ccgcatgtgt tgcaggcatt
    40921 ccgggaactg gaaggtgacc tgggctacgg catgttgctc tcccgctatg cgccggatat
    40981 gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc
    41041 gccggtattc tggagtttcc gcatcatggt gggctgtggt tccctgctgc tactggtgat
    41101 gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa
    41161 aatggcgctc tggagtttgc cgttgccgtg gattgcgatt gaagccgggt ggtttatgac
    41221 cgagtttggt cgtcagccgt gggcgataca ggacatctta ccgacatact ccgcgcactc
    41281 cgctttaacc acaggacaac tggctttctc actgatcatg atcgtagggc tttacaccct
    41341 gttcttaatc gccgaagtct acctgatgca gaaatatgcc cgtctggggc cgagcgcgat
    41401 gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa
    41461 acattgcgct tcatctggtg gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc
    41521 gacggatttg acatggggat cggctgtctg ctgccgctgg tggcgcgtaa tgatgatgaa
    41581 cgccggatag tgataaacag cgttggtgca cactgggaag gcaaccaggt ctggttgatc
    41641 ctcgctggtg gggcattatt tgccgcctgg cccagagtgt atgcagcggc gttttccggc
    41701 ttttatgtgg cgatgatcct ggtgctgtgc tcactgttct tccgcccgct ggcctttgat
    41761 tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt gggacgccgg tctggtcatc
    41821 ggcagtctgg tgccgccggt agtcttcggt atcgccttcg gcaacttgtt gctcggcgtg
    41881 ccgtttgcct tcacaccgca attacgcgtg gagtatcttg gcagcttctg gcaactgctg
    41941 acgccattcc ctttattgtg cggattgctc agccttggga tggtgatttt gcaaggtggc
    42001 gtctggttac aactgaaaac tgttggtgtg attcatctgc gttcacagct ggccaccaaa
    42061 cgcgctgcac tgttggtgat gctgtgcttt ttgctggcgg gttactggct gtgggtcggt
    42121 attgatggct ttgtactgct cgcccaggat gctaacggtc cttccaatcc gttaatgaaa
    42181 ctggtggcag tgctacctgg tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg
    42241 atcttcccgc tgctggggtt cttctgccca ttgctgacgg tgatggcgat ttatcgtggt
    42301 cgcccgggtt ggggattttt aatggcgtca ttgatgcaat ttggcgtgat tttcacggca
    42361 ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga gtccaatctc cagcctgacg
    42421 ttgtgggaca gcacttccag tcagctgacg ctgagcatta tgttggtaat cgtgctgata
    42481 tttttgccca ttgtgttgct ctacactctc tggagctact acaaaatgtg gggacgcatg
    42541 acaacagaaa ctctccgccg taacgaaaac gagttgtact aaggagcagg aacaatgtgg
    42601 tattttcttt ggttcgtcgg cattttgttg atgtgttcgc tctccaccct tgtgttggta
    42661 tggctggacc cgcgtctgaa aagttaacga acgtaggcct gatgcggcgc attagcatcg
    42721 catcaggcaa tcaataatgt cagatatgaa aagcggaaac atatcgatga aagcgatctt
    42781 aatcccattt ttatctcttc tgattccgtt aaccccgcaa tctgcattcg ctcagagtga
    42841 gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt catggtgtgc gtgctccaac
    42901 caaggccacg caactgatgc aggatgtcac cccagacgca tggccaacct ggccggtaaa
    42961 actgggttgg ctgacaccgc gcggtggtga gctaatcgcc tatctcggac attaccaacg
    43021 ccagcgtctg gtagccgacg gattgctggc gaaaaagggc tgcccgcagt ctggtcaggt
    43081 cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca ggcgaagcct tcgccgccgg
    43141 gctggcacct gactgtgcaa taaccgtaca tacccaggca gatacgtcca gtcccgatcc
    43201 gttatttaat cctctaaaaa ctggcgtttg ccaactggat aacgcgaacg tgactgacgc
    43261 gatcctcagc agggcaggag ggtcaattgc tgactttacc gggcatcggc aaacggcgtt
    43321 tcgcgaactg gaacgggtgc ttaattttcc gcaatcaaac ttgtgcctta aacgtgagaa
    43381 acaggacgaa agctgttcat taacgcaggc attaccatcg gaactcaagg tgagcgccga
    43441 caatgtctca ttaaccggtg cggtaagcct cgcatcaatg ctgacggaga tatttctcct
    43501 gcaacaagca cagggaatgc cggagccggg gtggggaagg atcaccgatt cacaccagtg
    43561 gaacaccttg ctaagtttgc ataacgcgca attttatttg ctacaacgca cgccagaggt
    43621 tgcccgcagc cgcgccaccc cgttattgga tttgatcatg gcagcgttga cgccccatcc
    43681 accgcaaaaa caggcgtatg gtgtgacatt acccacttca gtgctgttta tcgccggaca
    43741 cgatactaat ctggcaaatc tcggcggcgc actggagctc aactggacgc ttcccggtca
    43801 gccggataac acgccgccag gtggtgaact ggtgtttgaa cgctggcgtc ggctaagcga
    43861 taacagccag tggattcagg tttcgctggt cttccagact ttacagcaga tgcgtgataa
    43921 aacgccgctg tcattaaata cgccgcccgg agaggtgaaa ctgaccctgg caggatgtga
    43981 agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt acgcaaatcg tgaatgaagc
    44041 acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc attcagttac ctgaatgctc
    44101 tgaggctgat gacaaacgaa gaactgtcta atgcgtagac cggaaaaggc gttcacgccg
    44161 catccggcca ctttcagttt tactctttct cggagtaact ataaccgtaa tagttatagc
    44221 cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt gaggatagcg cctttaatat
    44281 tgacgcctgc ctgttccaga cgctgcattg acaaactcac ctctttggcg gtgttcaagc
    44341 caaaacgcgc aaccagcagg ctggtgccaa cagaacgccc cacgaccgcg gcatcactca
    44401 ccgccagcat cggcggcgta tcgacaatca ccagatcgta atggtcgttc gcccattcca
    44461 gtaattgacg catccgatcg cgcatcagca gttcagacgg gttaggtggc acctgaccgc
    44521 gagtaatcac atcaaagcct cctttgccaa aatgctggat cactttgttg agctcatctt
    44581 tacctgccag atattccgac aagccatgtt cattactcac ggtaaacagg ttatgcgaat
    44641 aaccacggcg taagtcggca tcaataaata acactttttg atcggactgg gcgatcaccg
    44701 ctgccagagt tgaactgaca aacgttttac cactgtctgg cgtcgcaccg gtgatcatca
    44761 gaatgttatt ctccgtctcc atcatagcga aatgcagact ggttcgtagc gcacgtacgg
    44821 cttccacagc agaatccgcc gggttatcca ccgccaggaa ggggatattt ttagtacgat
    44881 ggcgctgctg attagaaaat aaatttttct tacgcagacg ggtgcattta tccagccact
    44941 cggacattgg gatagtggca taaacgctga tgccgtgctc ttccagttgt tccggggctt
    45001 ctacaccacg acgcaacatc gcacgcgcca gcacggcacc cacagaaata aacaggccaa
    45061 gaataaaacc aagcaccaca ttcaacgctt ttttcggttt cactggctgc ggctgagtga
    45121 ctgccgggtc gataatccgc acgttaccaa tggcactgga tttcgaaata ctcaactcct
    45181 gctggcggtt aagtaattgc agatataccg cacggcccgc ttctacgtca cgacttaaac
    45241 gcaacacttc ctgttgggtg gaaggcattg ccgatacccg cttattcagg cgtttgcgtt
    45301 cttgctccag cgtctggcgt ttttccagca gcgcacgata agttgggtga tctttcttat
    45361 acagctggga gatctctgcc tcgcggaagg tcagctcatt gagttgatta tcaacgttca
    45421 caatctgctc aagaacggct ttggcttcca ggttaaggtc aaccgaatcg cgctgctggc
    45481 gataaacgtt gagtttttct tccgcctggt ccagctcgct gcgcacttca ggtaactggc
    45541 gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg gcgagcgata ttctgttgca
    45601 aatagttgtt agcgatgctg ttcagaatac gagtaatcaa ctggggatca tcaccagtca
    45661 tggtaagttc cagcatcccg ctttctttac tgcgttcgct aacggtaaag gtttcctgca
    45721 atgcgttaat cgcttccagt tcggtacgct ggctcaggac aaactgtgtt cctggtttgg
    45781 ccttaatgtc cgcgatagtc agcgcaacgc catctttttc cagacgctgg ccgaccatac
    45841 cattgacggt gaactcttca ccttccagtg tatagtggcc gttttcccca accgtgagtg
    45901 tcagttgctg atcctgacca ttcagttgtg gaatatgcat ccagctgatc gccagctcac
    45961 ctggtttttc tttggttaat ctcgcccagc cgcgacccac aatcggaaaa tacttctgct
    46021 caactatgtc gcgcagattc agttcagcaa tggttttacc gagaatcatg cgcgattgca
    46081 gcagttggat ctccggtgca gactcgggcg atgagttagg gatcatatcg ctcaggccgc
    46141 tgagaatggc gttgccctgt ttttgctcaa cctggaccag agtatctgcc tgataaattg
    46201 gtgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa taacgcggtc acgctgataa
    46261 taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc tcattttcct
    46321 gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat aaactcaaat
    46381 taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatacc gtagacatgt
    46441 tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc cagccattgc
    46501 ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg agcaatatgt
    46561 tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt gagcttacgt
    46621 ccggcatgtc catcaagaga tacgccatga ttagcggcaa catctgccgc tgtcgcatct
    46681 gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc cggcagacgt
    46741 ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca aaccaccagg
    46801 attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct gtcaggtcat
    46861 gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga gccagcggcg
    46921 cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg agtatcattg
    46981 ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc tgcttgtttt
    47041 gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc ataccctcag
    47101 cgttacccag cgcttccgcc agggtcatac cgctgcggtc cattttcagc gtgctctgtt
    47161 tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata aataaaatat
    47221 cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc gcatagaggt
    47281 tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct gcatctgcgg
    47341 tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg gtaatcggct
    47401 gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgagcgg aacgccgcga
    47461 cgctgacatc gacttgcggg ctttcaatga ctgaatccag acgcgctgta atctcgttgc
    47521 gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg tagaaaatcg
    47581 caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc cctgcggggg
    47641 tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atccccaatg ccgatacgat
    47701 attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata atcggtttgg
    47761 cgcgcaactg atcaatcaat cctggcgtga cgggatagat attgaccatt ttgtccaggt
    47821 catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa ttctgaccag
    47881 gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa aacttaaaaa
    47941 tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt acgtcaggtt
    48001 attgtccaac cggtacttct cgctctgccg tcattggata gagctggtat ttgcgtgaca
    48061 gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg ttgcggttag
    48121 gtccaatggt catcaagtcg aatggaatcg agatataaaa acctttacta aagccgcctt
    48181 cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc gccacgccgc
    48241 tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc ttttgccaga tattgcccaa
    48301 cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc gtcacgaaac
    48361 ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc ccagtcacgc tgcttcacgt
    48421 agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaagc agctcggaac
    48481 cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag aaaccattgc
    48541 ctaagtcagc aaaatagttc gcctgcaagt tgttgagata aacgtcattg cgaacgtagt
    48601 cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggcaacagc gaagacttaa
    48661 acttgtcgta gttgttgtaa atattggtga aaataccgcc atcaagcagc aggtggtcgg
    48721 taaaccagta gcgggcactg gacatcagcc ccagctggaa catatagaaa tcttccgggc
    48781 cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct tcacgaatac
    48841 ggtagccccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt tgaagttgct
    48901 ctgattgacc aggcgctgta ccagccagct gtttgcgcag gctggctacg tcggtttcgg
    48961 tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca acgccttgcg
    49021 gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagaa tcacggtatt
    49081 tgtattgttg accagacata tacagcgtct tatcgcgcag ctgaatttcc ggtgcttcaa
    49141 aacctgcgtt gtacttcagc gcggtaagtt ggttcgctac tgtggtgtac tgcaatcctt
    49201 cagattcagg cgcaggttga tatgccggtt ttggcgtatc gcgcagggca gggcgcagat
    49261 cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt gttaccgcgt tcataactca
    49321 ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc gacgttgaaa tggcttgcct
    49381 gaggcagttt gccagcgaaa tcattctggt aattgttgcc atcgtattcg agtttcagac
    49441 gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc gggccacgaa
    49501 agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga caatatttat
    49561 cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag ccccatgcca
    49621 tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatac tcaccgtcaa
    49681 acagacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc cagtaacctt
    49741 cttccgacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga tcgccgctga
    49801 aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg gtgccttcca
    49861 gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta tcccggtagt
    49921 tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac aatcctgtgc
    49981 cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact tcagcatgac
    50041 aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc ttcatcagtc
    50101 aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat atttttcggg
    50161 cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt ccacatgtct
    50221 tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa tcaccataac
    50281 gttattcttg tccgcgcctg caaggcgggg gtgatcttgc agataatcgg taacgctacg
    50341 gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag taatagtcac
    50401 cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc tgttctcatc
    50461 cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat tgagattgag
    50521 taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt ctgcttccca
    50581 ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt tcgccgcaga
    50641 gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt gtgtcaccaa
    50701 ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgccag gcagataaat
    50761 agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc tggcaatgaa
    50821 atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac gggaataacc
    50881 cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc gacccagaat
    50941 gtgttttgcc actgtttttc tggccgcgtg gaagtcacct cttcctgcca gacattacac
    51001 agcacattgc gacccgcaat attcagcgtc tctaatccag caaaggaaaa agtagaactc
    51061 agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt ccagcggctg
    51121 ccatctttta ttgccagcgc gttacgcaga gggtcctgcc cggaatttgt cacttccagc
    51181 aaattattat taagtttgac ggtttttaaa agacgtccat tgtgggtaac cagcatggcg
    51241 ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac cacaaagatt
    51301 cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg gatctgctga
    51361 tccgctacgg tgatatcctg attatcgaaa aggctggcgc gaaatgtatc gaccatactt
    51421 tgctggctgt acgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat aagagggcgc
    51481 acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc ggtacgaata
    51541 tcgcaaccgg gtatcattta actttaattt cgacgcgtta aagcagtatt agcgtgtagt
    51601 ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg ctgcaacaac
    51661 tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg tgccgacagc
    51721 gccagcagcc acgccaccag tggtagtagc agtcgtggtt gcgccggtag tggtcgttgt
    51781 ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt gcttcagggg cagcaaacgc
    51841 agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt tcataacttt
    51901 tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct actttgagta
    51961 taacatcctt gtgaaatctc cctttcctca taccgcttgc actatatact gcgggtagga
    52021 aaggcgcgca gagggaaata taagattgtt aactatcccc ctctggaaat acgacttgtg
    52081 agcataagat aaatcttagc aaaatacaaa ttaaagat
//
LOCUS       DAMZII010000019        49716 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZII010000019 DAMZII010000000
VERSION     DAMZII010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.019"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            66..1505
                     /gene="norV"
                     /locus_tag="POA77_001073"
     CDS             66..1505
                     /gene="norV"
                     /locus_tag="POA77_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDI6364541.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1502..2635
                     /gene="norW"
                     /locus_tag="POA77_001074"
     CDS             1502..2635
                     /gene="norW"
                     /locus_tag="POA77_001074"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDI6364542.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKIDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2763..5015)
                     /gene="hypF"
                     /locus_tag="POA77_001075"
     CDS             complement(2763..5015)
                     /gene="hypF"
                     /locus_tag="POA77_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDI6364543.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGAMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5168..5695)
                     /gene="hydN"
                     /locus_tag="POA77_001076"
     CDS             complement(5168..5695)
                     /gene="hydN"
                     /locus_tag="POA77_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDI6364544.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5844..6854)
                     /gene="ascG"
                     /locus_tag="POA77_001077"
     CDS             complement(5844..6854)
                     /gene="ascG"
                     /locus_tag="POA77_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDI6364545.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7114..8573
                     /gene="ascF"
                     /locus_tag="POA77_001078"
                     /pseudo
     CDS             7114..8573
                     /gene="ascF"
                     /locus_tag="POA77_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
     gene            8582..10006
                     /gene="ascB"
                     /locus_tag="POA77_001079"
     CDS             8582..10006
                     /gene="ascB"
                     /locus_tag="POA77_001079"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDI6364546.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10120..10539)
                     /locus_tag="POA77_001080"
     CDS             complement(10120..10539)
                     /locus_tag="POA77_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6364547.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10610..11365)
                     /locus_tag="POA77_001081"
     CDS             complement(10610..11365)
                     /locus_tag="POA77_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364548.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRVD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(11408..11878)
                     /gene="hycI"
                     /locus_tag="POA77_001082"
     CDS             complement(11408..11878)
                     /gene="hycI"
                     /locus_tag="POA77_001082"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDI6364549.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(11871..12281)
                     /gene="hycH"
                     /locus_tag="POA77_001083"
     CDS             complement(11871..12281)
                     /gene="hycH"
                     /locus_tag="POA77_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDI6364550.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12278..13045)
                     /gene="hycG"
                     /locus_tag="POA77_001084"
     CDS             complement(12278..13045)
                     /gene="hycG"
                     /locus_tag="POA77_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDI6364551.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13045..13587)
                     /gene="hycF"
                     /locus_tag="POA77_001085"
     CDS             complement(13045..13587)
                     /gene="hycF"
                     /locus_tag="POA77_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDI6364552.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(13597..15306)
                     /gene="hycE"
                     /locus_tag="POA77_001086"
     CDS             complement(13597..15306)
                     /gene="hycE"
                     /locus_tag="POA77_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDI6364553.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15324..16247)
                     /gene="hycD"
                     /locus_tag="POA77_001087"
     CDS             complement(15324..16247)
                     /gene="hycD"
                     /locus_tag="POA77_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDI6364554.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16250..18076)
                     /gene="hycC"
                     /locus_tag="POA77_001088"
     CDS             complement(16250..18076)
                     /gene="hycC"
                     /locus_tag="POA77_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDI6364555.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18073..18684)
                     /gene="hycB"
                     /locus_tag="POA77_001089"
     CDS             complement(18073..18684)
                     /gene="hycB"
                     /locus_tag="POA77_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDI6364556.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(18809..19270)
                     /gene="hycA"
                     /locus_tag="POA77_001090"
     CDS             complement(18809..19270)
                     /gene="hycA"
                     /locus_tag="POA77_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDI6364557.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19482..19832
                     /gene="hypA"
                     /locus_tag="POA77_001091"
     CDS             19482..19832
                     /gene="hypA"
                     /locus_tag="POA77_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI6364558.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            19836..20708
                     /gene="hypB"
                     /locus_tag="POA77_001092"
     CDS             19836..20708
                     /gene="hypB"
                     /locus_tag="POA77_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDI6364559.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            20699..20971
                     /gene="hypC"
                     /locus_tag="POA77_001093"
     CDS             20699..20971
                     /gene="hypC"
                     /locus_tag="POA77_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDI6364560.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            20971..22092
                     /gene="hypD"
                     /locus_tag="POA77_001094"
     CDS             20971..22092
                     /gene="hypD"
                     /locus_tag="POA77_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDI6364561.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            22089..23099
                     /gene="hypE"
                     /locus_tag="POA77_001095"
     CDS             22089..23099
                     /gene="hypE"
                     /locus_tag="POA77_001095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDI6364562.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23173..25251
                     /gene="flhA"
                     /locus_tag="POA77_001096"
     CDS             23173..25251
                     /gene="flhA"
                     /locus_tag="POA77_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDI6364563.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(25288..25641)
                     /locus_tag="POA77_001097"
     CDS             complement(25288..25641)
                     /locus_tag="POA77_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDI6364564.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(25673..25852)
                     /locus_tag="POA77_001098"
     CDS             complement(25673..25852)
                     /locus_tag="POA77_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364565.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            25928..28489
                     /gene="mutS"
                     /locus_tag="POA77_001099"
     CDS             25928..28489
                     /gene="mutS"
                     /locus_tag="POA77_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI6364566.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEEISPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28595..29251
                     /gene="pphB"
                     /locus_tag="POA77_001100"
     CDS             28595..29251
                     /gene="pphB"
                     /locus_tag="POA77_001100"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI6364567.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISTGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(29302..30069)
                     /locus_tag="POA77_001101"
     CDS             complement(29302..30069)
                     /locus_tag="POA77_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6364568.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHLVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            30265..31173
                     /locus_tag="POA77_001102"
     CDS             30265..31173
                     /locus_tag="POA77_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6364569.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            31170..32432
                     /locus_tag="POA77_001103"
     CDS             31170..32432
                     /locus_tag="POA77_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDI6364570.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            32429..33067
                     /locus_tag="POA77_001104"
     CDS             32429..33067
                     /locus_tag="POA77_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDI6364571.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            33072..33848
                     /locus_tag="POA77_001105"
     CDS             33072..33848
                     /locus_tag="POA77_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDI6364572.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            33937..35301
                     /locus_tag="POA77_001106"
     CDS             33937..35301
                     /locus_tag="POA77_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDI6364573.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(35340..35576)
                     /locus_tag="POA77_001107"
     CDS             complement(35340..35576)
                     /locus_tag="POA77_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364574.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(35587..37014)
                     /locus_tag="POA77_001108"
     CDS             complement(35587..37014)
                     /locus_tag="POA77_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDI6364575.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(37014..37607)
                     /locus_tag="POA77_001109"
     CDS             complement(37014..37607)
                     /locus_tag="POA77_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDI6364576.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDKFGLEHPHARRWQGLPQARNFSQENE"
     gene            37754..38161
                     /locus_tag="POA77_001110"
     CDS             37754..38161
                     /locus_tag="POA77_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6364577.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(38282..39274)
                     /gene="rpoS"
                     /locus_tag="POA77_001111"
     CDS             complement(38282..39274)
                     /gene="rpoS"
                     /locus_tag="POA77_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDI6364578.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(39337..40476)
                     /gene="nlpD"
                     /locus_tag="POA77_001112"
     CDS             complement(39337..40476)
                     /gene="nlpD"
                     /locus_tag="POA77_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDI6364579.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(40616..41242)
                     /gene="pcm"
                     /locus_tag="POA77_001113"
     CDS             complement(40616..41242)
                     /gene="pcm"
                     /locus_tag="POA77_001113"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDI6364580.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(41236..41997)
                     /gene="surE"
                     /locus_tag="POA77_001114"
     CDS             complement(41236..41997)
                     /gene="surE"
                     /locus_tag="POA77_001114"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDI6364581.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(41978..43027)
                     /gene="truD"
                     /locus_tag="POA77_001115"
     CDS             complement(41978..43027)
                     /gene="truD"
                     /locus_tag="POA77_001115"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDI6364582.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(43024..43503)
                     /gene="ispF"
                     /locus_tag="POA77_001116"
     CDS             complement(43024..43503)
                     /gene="ispF"
                     /locus_tag="POA77_001116"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDI6364583.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(43503..44213)
                     /gene="ispD"
                     /locus_tag="POA77_001117"
     CDS             complement(43503..44213)
                     /gene="ispD"
                     /locus_tag="POA77_001117"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI6364584.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(44232..44543)
                     /gene="ftsB"
                     /locus_tag="POA77_001118"
     CDS             complement(44232..44543)
                     /gene="ftsB"
                     /locus_tag="POA77_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDI6364585.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(44737..45060)
                     /locus_tag="POA77_001119"
     CDS             complement(44737..45060)
                     /locus_tag="POA77_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDI6364586.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(45110..45715)
                     /gene="cysC"
                     /locus_tag="POA77_001120"
     CDS             complement(45110..45715)
                     /gene="cysC"
                     /locus_tag="POA77_001120"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDI6364587.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(45715..47142)
                     /gene="cysN"
                     /locus_tag="POA77_001121"
     CDS             complement(45715..47142)
                     /gene="cysN"
                     /locus_tag="POA77_001121"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDI6364588.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(47144..48052)
                     /gene="cysD"
                     /locus_tag="POA77_001122"
     CDS             complement(47144..48052)
                     /gene="cysD"
                     /locus_tag="POA77_001122"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDI6364589.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            48304..49341
                     /gene="iap"
                     /locus_tag="POA77_001123"
     CDS             48304..49341
                     /gene="iap"
                     /locus_tag="POA77_001123"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDI6364590.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        11696 a        13565 c        13053 g        11402 t
ORIGIN      
        1 actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg
       61 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt gggtcaacgt gactgggaag
      121 tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagctac aacagctacc
      181 tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat
      241 ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc
      301 atgcagaaga ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc
      361 cgatctactg tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt
      421 ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc aaacagctca
      481 tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg
      541 acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
      601 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc
      661 tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac
      721 cagtcgatat gatagccact tcccacggcg tggtatggcg cgataacccg acgcaaattg
      781 tcgagctgta cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg
      841 acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa
      901 ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
      961 tgactaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga
     1021 tgatgccgaa aatcgccggg ctggtggaag agatgactgg tttacgcttc cgtaacaaac
     1081 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc
     1141 gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc
     1201 aggacgctct ggagttatgt cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
     1261 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga
     1321 cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
     1381 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc ccggataact
     1441 tcctctgccc ggaatgctcc ctcggcaaag acgtctttga cgaactggca tcggaggcaa
     1501 aatgagtaac ggcattgtga tcatcggttc gggcttcgcc gcccgccaac tggtgaaaaa
     1561 tattcgcaaa caggacgcca ctattccatt aaccctgatt gccgccgaca gcatggatga
     1621 gtacaacaaa cctgacctca gccatgttat cagtcagggg caacgtgccg atgaccttac
     1681 ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cacctgtttc cacaaacctg
     1741 ggtgacggat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc agtggcaata
     1801 cgacaagcta gtactggcaa ccggtgccag tgcctttgtc ccgcctgtgc ctgggcgtga
     1861 gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga
     1921 tgcccgacgc gtgttgattg ttggcggtgg tttgattggt agcgaactgg cgatggattt
     1981 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt
     2041 aatgccaccg gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct
     2101 gttgttgaaa tctcagttgc aggggctgga aaaaatagat tctggcattc tggcaacgct
     2161 ggaccgccag cgcagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
     2221 aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
     2281 tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa ttaacggtca
     2341 ggtattgccg ttcctccagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
     2401 cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt
     2461 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga
     2521 acgccaggga atggtggctc gcggtgttga cgatgctgac cagcttcgcg cctttgtggt
     2581 cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccga tgtaggtggg
     2641 ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc
     2701 atgtgtaggc cggataaggc gtttacgccg catccggcaa tggtgtccaa atgcaacacg
     2761 ttttatccgt tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc
     2821 agagacaaac cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg
     2881 agataatgcg ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc
     2941 agcgtagtga taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc
     3001 agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt cagccactgc
     3061 tgccagaaag tggcgagatc cagttgattg tccaccagcg gcattgtcac cggatgcgtc
     3121 actccgtggc acgaagctgc gagtgcctcc agagcacaag ccgcttcacc ttcataactt
     3181 aacgtggctg gcgcacagcc cagtgccgcc gccacagcat cgaacaaccg cccacacgat
     3241 gacgccagcg gcgcgttaat tccacgctca atggcccgcg ccagcacgct ccagttttgc
     3301 tgttgcacac ttgctgtttc agaataattc tgccactccg gcacaaagcg caggcactgc
     3361 gccagcaggt ttcgccacgg ctgcttcgcc gccaaatcgc cacccggaag cgccactgca
     3421 ggcaagccgc ccaggtgctc acattcgcga tagttcaccc gcagacactc gccgccccac
     3481 aaagcgccgt tctcccccat accgatacca tcgagcgtca aagcaatgac atcaccgcca
     3541 tccagcggcc actgatgctc tgccagacac gccgctgcgt gggcatgatg atgcagcacc
     3601 gtttgcgtcg gcatattcat ttcgctcgcc cactggctgg agacatagcc cggatgcacg
     3661 tcatgcacaa cgtattgcgg agtgaaatcg tagatgtttt gcatcaggcg taacgcttcg
     3721 cgccactgca tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct
     3781 tgttcaccgc gcaccaggca gaaggtgttt ttcagatccg cgccaagaca cagcacaggc
     3841 ggaacatttt taaagcccgg aggcaaaacc agcgcatccg gcacataccc gcgtgaacgg
     3901 cgcagcattt cgccgctttc gcgcaccacc gaatcatcca tccgctgcac gatgtcgcgg
     3961 ttatgtatca agaatccgtc ggcaatgccc tgcaaatcct ccagcgcctg ttcgttgctg
     4021 atagccggtg gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc
     4081 tgtaacagca aatgctggag cgggttcgca ggcaacattg ccccgacttc gttaaggtca
     4141 ggggcgatat catcacaaag ctcaggaacg tattttttat ccaccagcac aatcggcgcg
     4201 gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac cgtcagccac tggcaacatg
     4261 accgccagcg gtttcgccgg gcgatgtttg cgcgcccgaa gtgtcgccac cgcgttactg
     4321 ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc
     4381 atttttaact gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg ttcaccatga
     4441 cttacccatt caagatgtgg gccacactcc gggcaggcca ccggctgggc gtggaagcga
     4501 cgatcgagcg ggtcgcggta ctctttgtca caagccggac acaacggaaa cgccgccatc
     4561 acggtaaacg ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg gccgcagtgg
     4621 gtacagttga taaacggata acgataacgc cgttcgcctg gggtattcat ttcggcaaga
     4681 caggcagggc aagtagcggc atcgggaaca atttgcgtat tcatggtgcc gcctgtgctc
     4741 tggcgtatag tgaactcggt gggcagttgt gaccagatgt acggctcacg ctcgacgcta
     4801 tcaatacgcg ccagcggcgg gcagtgctga tgcaattgaa caagaaacgt ttccgggtct
     4861 tccagcagcc ggacttctac gccatcgccg tcattacaga catcgccgtg aagatttaat
     4921 tgctgtgcca gctgccagac aaacggacga aaaccgacgc cctgcacttt gcctcgaata
     4981 cgcagttgga caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt
     5041 gcgtaacccg ccctgccgga cacgactgcg tcgtatccgg cagtctcagg tcggcgatac
     5101 cgccagctcc gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctat
     5161 aagattttta gaacatcagc gccgtacggc gacgtttttc tgcgctcagt tgttcaagtt
     5221 tattacgatc gacacaaatc agcgcatggg tcgggcaagc cgccatacac gccgggccgt
     5281 cttcgcgatg gttgcacagg tcgcatttat tggcttcagc tttgtcagcc cgtacattca
     5341 gccccgcgcc gctgttgcgg atcaccggac gtaccaccac ttccatcgcg ccatacgggc
     5401 aagccaccac gcaggttttg caaccaatgc aacgttcctg catcacatga acaaaccctt
     5461 tatcacggct gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac
     5521 ggcagactgt cgccgtggaa atgttcacac ctttaatgac atggattcgc ggtaaaaagg
     5581 tttccggggt cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt
     5641 cacaggtacg gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat
     5701 tctccagcaa tgacagttaa tgcgccgata cattcacaaa tcatgccagt ttttaatttg
     5761 ctgttattta aggaaattaa tttctgtaat gcaggaaaaa agatgtcatc gacactagtg
     5821 acgatgacat gtgatgacaa tgtttatcgc gaaagggcaa tgagtgagtc gcggcggatc
     5881 agttttccgc tgaaggtttt cggcggtgag aaatccccgc catcgagcat aaaaatcagc
     5941 cgtccaataa tttcctgaat catctctgtt accggaattt ttacgctgga gagcgccgga
     6001 acggtgtagg gggcaattgc gatatcatcg aatccgataa ccgacacctg ctctggcacc
     6061 gctacgccgc gctcgtgcaa cgctttgatc gcacctatcg ccatatcgtc gttactggca
     6121 actaacgcgc taaatttagc cccacgttcg agcaacgttt ctaccccttc ggccccgctg
     6181 gcgggcgtcc atttaccgtt agcgataagt ttttcattga gcgcaatacc atgctgcgac
     6241 agcgcgtctt tatacccggc aagacgttca acgctggtgg gggaatccat cgagccggta
     6301 aggaaagcaa tctcctgatg tccggcgttt atcaactctg ccacggcatt aaagctggtc
     6361 tgtttatgat cgcaccagac gctatggctg ctgtttttgc gcaggcggcg attaagcacc
     6421 attatcggct gactgtgcgc gtcaatgatg tcatcaatct catccacgct taaaaaacgc
     6481 gggtaaatca taatggcgtc gcagcgcaaa tccagcagat actgaatcgc ctggcgctct
     6541 tcttcggcgc tgtgtttacc atctgccaat agcaactgcc gccctttctc ttccgccatt
     6601 cgcgcggcat gaaacagtaa ttcactaaaa taaatgccgt gataaagcgt gttggtcact
     6661 accagcccca gcgtctgagt actcttcgcc gacagattgc gcgccagcaa atttgggcgg
     6721 taaccgctct cttctaccgc ctgaaacacg cgatctttag tctcctggct gacgtagcca
     6781 ttacctgaaa gcacgcggga aacggtcgct tttgaaaccc cggcgcgctt cgccacttcc
     6841 agcatcgtcg tcatcatttt catcccttta cacgcaatca acgcagtgta ctgcaccgtt
     6901 tgccgattgt ccttgcgcaa tcggcgggaa aaatattcag gtgaccggtt tcacaaatat
     6961 aaaaaatgaa caattaactc tcttgcttat ttagtgacaa ctattcatga ttttgtgaaa
     7021 ccggtttcct aattccgttt cagcatcggc atttttccgt tacgtcgact gataacaact
     7081 acatctaccc tactgataac aggataaaat ccgatggcca aaaattatgc ggcgctggca
     7141 cgctcggtga tagcggcact gggcggcgtt gataacatct cggcagtcac gcactgtatg
     7201 acgcgtttgc gctttgttat caaagatgac cagcttatcg acagcccgac gttaaaaacc
     7261 atccccggcg tgctcggcgt ggtacgtagt gacaaccagt gtcaggtgat tatcggcaat
     7321 accgtttcac aagcctttca ggaagtcgtc agcctgctgc cgggagatat gcagcccgca
     7381 cagcccgtgg gtaaacccaa actcacgctg cgtcgcattg gtgcggggat cctcgatgcg
     7441 ctgatcagca ccatgtcacc gctgatcccg gcgattatcg gcggatcgat ggtcaaactg
     7501 ctggcgatga tcctcgagat gagcggcgtg ttgacaaaag gatcgccgac cttaaccatt
     7561 ctgaatgtga ttggtgacgg tgctttcttc ttcctgccgc taatggtcgc ggcatctgcc
     7621 gccatcaaat ttaaaaccaa tatgtcgctg gcgattgcca tcgcaggtgt gctggtacat
     7681 ccgagcttta ttgaactgat ggcgaaagcg gcccagggtg aacatgtcga atttgccctg
     7741 attccggtca ccgcggtgaa atatacctac acggtgtgat cccggcgctg gtgatgacct
     7801 ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac accggctgtg accaaaaact
     7861 tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc
     7921 cgattggtat ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc
     7981 tgggctggct ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga
     8041 tgcaccgcgt ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga
     8101 tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg
     8161 cgtggaaaac gaaaaacccg gaactgcgcc agacggcgct ggctgcggcg gcatcagcca
     8221 ttatggcggg gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc
     8281 ttatcgccag tcttatcagc ggttttattt gcggcgcggt tgccggtatg gcggggcttg
     8341 ccagccactc aatggcagcg ccggggttat ttaccagcgt gcaattcttc gatccggcga
     8401 atccaatgag catcgtctgg gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta
     8461 tcctcacact attgctcggc tttgaggata ttcctgttga ggaagcggct gccgaggcgc
     8521 gaaagcatca gagcgcacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa
     8581 aatgtcagta tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga
     8641 aggtgcgttc cgtgaaggtg gcaaaggact gaccacggtc gatatgatcc cgcacggcga
     8701 gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt
     8761 ttatcccagc catgaggcga cggattttta tcatcgttat aaagaagata tcgccctgat
     8821 ggcagagatg ggattcaagg ttttccgtac ctcaattgcc tggagccgcc tctttccgca
     8881 gggcgatgaa atcacgccca atcagcaggg cattgctttt taccgttctg tctttgaaga
     8941 gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc cacttcgatg tgccgatgca
     9001 tctggtcact gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta
     9061 cgccagaacc tgctttgaag catttgatgg tctggtgaaa tactggctaa ccttcaatga
     9121 aatcaacatt atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga
     9181 aaatcaggat caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc
     9241 caccaaaatc gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg
     9301 taacttctac ccttacagct gcaaaccgga agatgtctgg gcggcgctgg agaaagatcg
     9361 ggaaaacctg ttttttatcg atgtgcaggc gcgcggcgcg tatccggctt actctgcccg
     9421 cgtatttcgc gaaaaagggg taaccatcaa caaagcaccg ggcgatgatg aaatcctgaa
     9481 aaacaccgtc gattttgtct ctttcagcta ttacgcctcg cgctgcgcct cggcggagat
     9541 gaacgccaac aatagcagtg cggcgaacgt agtgaaatcg ctgcgtaacc cgtatctgca
     9601 agtgagcgac tggggctggg gtattgatcc actcggtctg cgtatcacca tgaacatgat
     9661 gtacgaccgt tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga
     9721 acttgctgcc aatggcgaga ttaacgatga ctatcgcatc agctatttac gcgaacatat
     9781 ccgcgcaatg ggcgaagcga ttgcagatgg cattccgctg atgggctaca ccacatgggg
     9841 ctgtattgat ttagtttccg cctctacggg tgaaatgagc aaacgctacg gttttgtcta
     9901 cgtagaccgt gacgacgcag gcaacggcac gctgacgcgc acgcgtaaga aatcgttctg
     9961 gtggtataaa aaagtaattg ccagtaatgg ggaagattta gagtaagtaa ccgtgccgga
    10021 tgcggcgtga acgccttatc cggcctacat agttcgtgcg acatgtaggc ccgataagcg
    10081 cagcgcatcg ggcaatctgg ggtttggcat cagtcaaaat caaagaaacc attccggctt
    10141 aacgccaaat cgggctgaaa gtgccttaat gtgggatatt gtcagggatc gttgtccaga
    10201 caaaatctga ctgaccaggg atttagagcc gatttcgttc ttcaaatccg cataggtcag
    10261 attatgttga tcaatcaacg tgcgaagtaa cgccacgcca accggcattg ccgcaacggc
    10321 tttgtcgaac tctgcaaact tttcgttgtt gttttcatac tctgcaatgc ggctcgccag
    10381 aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa tattcgacca gttccagtgc
    10441 ttcacgataa tcatcttctg agtcactgcc tcccagaaag gggactgcat tgaccagttc
    10501 tcttgttgct ttaacagcgc gtgcagcatt agcagtcatt ctttgttctc tctgtagtaa
    10561 cgtgacgtct gtctgcacgt aatccccctc atagccactc tgcaaaaagt cagttctggt
    10621 tcaccccata aagcacatcc ccctttatca agcaacgctg cgtctgcgcc agatcttccg
    10681 tttgcgggtc gcgcccggcg atgggtttga ggacgagttt acggttgggg gtataagtaa
    10741 aacgcgtgag cgcgggatag cttaacttgc caaaccagcc gtgaacacgc tgccatttgg
    10801 cgtcttttac ctgatacaga ctggttatcc agtaaccgga atcaaactgc atatccagca
    10861 attgcgtgtg gccattcctt tgtaaatcca gcaaatcatc aatgccagtt tcacttgcgg
    10921 tataaaaacc atcggactga aaaacacacg ggcgaccttg ctgattaaag gtcatcagaa
    10981 tcaaaaacgc gttaggtgcc agaccatttc ctgaaatacc ccgccagata accagatcct
    11041 gaatgccgtt acgatcaaga tcgacacggt aaagttgcgc attacccgcc atattttgca
    11101 gcacccccac aggcacagac caggatttac cggcgcgatc tttaccgttc aattgccatt
    11161 cactatcatc atttggcatc ttttcaagcg tcacgccagc aacgccgact tttctaaaat
    11221 gccagttttg ctcggcatca taaggcatgg tcaggagagg tttcagcgcc cgcttttgcg
    11281 cgtattcgct gtcactcatc ggcagatatt cggtaaagct ggtggcctgt gcaggcagca
    11341 ctaagctcaa taaaaaagcg cacatccctg ttctgatgtt caattccctg aattccttaa
    11401 tgaaaaacta ctctacttcc accgctaact gcgcaaatcc accatttcct tcccagcctt
    11461 ccagtcgttg ataaacggtt tcaaccgcat ctttaatcgg ctgggtcatc gggtagtaaa
    11521 agccgacgat atccggctgg atgccgagga aaatcacttc gccaatatct tctttcaact
    11581 ggtcgataag gtagttcaac ggcatgttat gggtagtcat cataaacatc tcggcgatat
    11641 catccgggtc gatgatgcgg atctcgccgg ggtttagccc catatcagtg gcgtcgacaa
    11701 tcagcagtcg cgtagggcgc agttcacgga tggcgacgat gtcgttttcc ggtgcactac
    11761 cgccgtcaat caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg
    11821 ggcctgcgcc atcatcgccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc
    11881 agtctcctca ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttagc
    11941 agcattgtac tccattcctg ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg
    12001 ttggccagca tcggcacatg gttaatgtcg atgacgatct caccatattt cggcacgcct
    12061 tccattttgc ggcgggcttc gctgcccgcc tccagagtgg caatccatgc cagatattca
    12121 tcccacgggc aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc
    12181 gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata
    12241 aatttacggc tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcta
    12301 caacatgatt cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc
    12361 agcgtgcaac ctgttcttcg ccctgcccta actgtgtcag gaagtcatcg gcaatctgac
    12421 gaccgtaacg ataacctgcc aggcggcgtg cttcgcgatc cactttcaca cgcagcggct
    12481 gcaccatatc gccatgcagg atttccgccg gttgttcatc ctgctcaccc gggccacggg
    12541 cgtgaatttt ctgctccagc aggccgagcg ccattgcaaa gccatacagc gtggcggcag
    12601 gcgttggcgg gcaaccaggg atgtagacat ctaccgggac gattttatcc gtaccgcccc
    12661 acacgcagta gagatcgtgg aagatcccgc cactgttacc gcaggcaccg taggagatac
    12721 agattttcgg gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg
    12781 tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc
    12841 cgaagcgttc tgcatcaaac agcggcgaaa gcgtggcgaa aatttcgatt tcgcagccgt
    12901 tgcagccgcc gcagtccacg cggtaaacgt aggcagaacg tttgattttt ttcagtaacg
    12961 acgccttcat gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt
    13021 cacgggggcc taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat
    13081 acggtcggac ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc
    13141 gcggtggttt tccgcgcggc tgtcaccatt gtgcttaagc agcgcaatgg cgtagtcgat
    13201 ctctttctgg acggcgaaag gacgattgca gacgcgacag ctgcacagca caaaacggga
    13261 ctgttgcagg aaatcttctt tcttccacac cgccagttcg tactcttgcg acagtttgat
    13321 cgccgccgtc gggcaaactt cttcgcagcg tccacagaag atgcagcgcc cgagattaaa
    13381 ctcccacgca agctctcctg tggcgaggtc ggtttcaacc gttaaagcgt ttgacgggca
    13441 ggcattgacg caggccgcgc agccgatgca ctgctgcggg ttctgctctg gcttaccacg
    13501 gaagttttta tcaaccgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt
    13561 gatgactttt ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg
    13621 ctcaatgctg taacgctcga gttctttgta cggcaccact ttgctcttct tcttacgtac
    13681 atcgaccacc gtcatgcggt cggtacagga gtagcaaggg tccaggctac cgataatcag
    13741 cggcgcatca gaaacggtgt tgccacgcag catgtagcgc agggtcggcc agttcgcgta
    13801 ggtcgcggca cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg
    13861 gatatcgtcg ccgcgcggcg cttcggcaaa gcccagcgcg aaacggtgcg gaatgtaggt
    13921 aaagccttcc accatcaacg gaccgcccgg caggttatcc agaccgtagt cgatcatgtt
    13981 cagcgcggta tagacctcgt tgattcgcac tttcagacgg gaaataacgt cgcagccctg
    14041 ctcgctgtgg acttccattg gcagcaggcc ataaccaaca aacgggtgat cggcgcgggt
    14101 atcgcgagcg tgaccgctgg cacggaccat cgggccaacg ttactgaagt cgcgggcaat
    14161 ttccgggtcc agacgaccaa tgccgacggt gcgctgttcc atgttcggcg tactcagcag
    14221 cacatccacc agctcctgca cttcacgacg catctgttgc gccagctggc gggtctggat
    14281 catgtcatct ttcaacagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt
    14341 acgcgcaccg gtgaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg
    14401 cataaagccg gagtcaaagc cggtaaagtg gcaggcgaga ccgaggttga gcagatgcga
    14461 gtgcaggcgt tccacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg
    14521 aatacccatc gcgttttcta ccgacgtggt gtaggcggtg ctgtgggcaa agccgcagat
    14581 cccgcacaca cggtcagaga ggaaggtcac ttcgttgtaa cccatacggg tttccgccag
    14641 tttttccatg ccgcgatgaa cgtagaacag acggtagtcg gcgtcgataa tgttttcgcc
    14701 atcgacgaac agacggaagt ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg
    14761 cacgacgttg tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt
    14821 agtcggtgcc ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc
    14881 cggccagtca tccggcagca ccagacgacg ttcatccggc agaccaaccg gaatcaaacc
    14941 gtacatatcg cgcacttcgc gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga
    15001 cggatactcc ggtttgttgg cgtcaacttc gacgcgaacc gttacccaac acttggtgcc
    15061 cttctccatc gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt
    15121 accaaacagc accgacagcc agccaccctg cttgtagtag agaaactcca ccacttccgg
    15181 caggtagttc accttcacgg tcacggtcag ctgatctttg gtctgccagg cgtggtccag
    15241 cacgacgccc ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc
    15301 agacatgctc aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca
    15361 aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg
    15421 ttttcgaaca gcgcgataac caggacgccg acgaccagtt tgacgatggc aatcaccagc
    15481 gccagcagca gcccaccggc ggtgaaggtt tccatctgtc cccacgggat aaatacgccg
    15541 acgaacatct gcagcaccac cagctgtttc aggctgatac cccatttcat gacgccaaag
    15601 ccgctgccgc tgtattcaga gagcgggcct tcctgcagct cctgctcggc ttccgccagg
    15661 tcgaacggca gtttgcccat ttcgataaag gtggcgaacg cacaggcaca aagcgccagt
    15721 accagcggga tgctctggct cagcggccag tgataaacgg tgtcggtgat gttgctgatg
    15781 ttggtggaac cggcaacctg tgcggcgacc cacagaccaa gcagcagcat cggttcgacc
    15841 agcacgccaa gcatcgcttc acggctcgcg ccgatagcgg taaacgggct accggtatcc
    15901 agaccggaaa tggcaaagaa gaaacgcgcg atggcaaaga gatacagtaa ggtgattaaa
    15961 tcacccagtt gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc
    16021 agcatgacgc ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg
    16081 ccaacgctct ggcggcccag cagtttgaaa atgtcacgat actcctgcaa cacacccggc
    16141 ccacgacggt tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca
    16201 acggcaaata acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    16261 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    16321 acgcactacc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    16381 gtttcagcac tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca
    16441 ccattgattt ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca
    16501 ggcggtcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    16561 gcaatgtgat catgggttga gaaacggtgg tgttagcagg ctccagcggc agaggtacag
    16621 cagcagagag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    16681 ccagtgccac tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt
    16741 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    16801 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    16861 cgccactatt gctcagtttg aaaaaggatt gatagataac ccattccccg gcaaacccgt
    16921 tcagcggcgg cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg
    16981 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    17041 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    17101 gaccaccgac cagaccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc
    17161 cagcgcccag ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagcaggc
    17221 gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg atcatgccga
    17281 gcaccagcag cgcgatcccc caccacagcg gcgcgttacc acccagcagc gacagggtta
    17341 aaatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag
    17401 aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gccagcaggc
    17461 caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca
    17521 tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag caagcaatcg
    17581 ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc
    17641 tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac atgccgaggt
    17701 tgctggcaat gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc
    17761 acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac agaccgcaaa
    17821 ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg tagcttacca
    17881 gcgacagcgc accgctcacg ccaaccgcgc cagtcagcac agtgaagccc gcggctgccg
    17941 tatacaggct accaaccgcg ccgccaattc cggctatcca gccacttagc gtcttttgaa
    18001 aagaaaagag aaatgccaga acagcggcgg caataaacca cgccacgccg ctattgatca
    18061 gggaaattgc gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc
    18121 aaagtccgta ttaaaagtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt
    18181 atccaccaga tgcagggctt tggtcgggca catccgcacg caggccggac cttgttcatc
    18241 aaaactgcaa aggtcacatt tgacggcgat cgcgcgaata cccggcaccc agtcaagcag
    18301 cgtactgata cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc
    18361 cggaatatcc agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc
    18421 gcacagcttg cagcttacgc acaggctttc attcaactgc acggccccat caacgcgggt
    18481 gatggcgtta accgggcaga ccaccgcgca gggtgcatct tcacagtggt gacagagctg
    18541 cggcgcagat tctttttcat tcagcatcac tctcaggcgc ggcattgatt gcaggccgtg
    18601 ctggcgatgc gtctctgaac aggcggcctc acaagtgtgg cagccgatac agagcgtgga
    18661 gtcagcaatt acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa
    18721 acatgccgat gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta
    18781 acgcctgcaa aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt
    18841 ggcttaaatt caccggcaca ttgttttcaa tagcggtgta taagctgtcg taaacgccag
    18901 cgattttttc gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat
    18961 caatcatgcc gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt
    19021 ttgtttcgac gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa
    19081 tgcgaaaatg ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt
    19141 gatgcaggcg cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc
    19201 actggtcctg taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc
    19261 aaatagtcat tgtcaggtta cccgtttaac agagatgcca gctttaagca ttttctgtgc
    19321 caacttttaa tttattgtta ttaaagagat ttttaagcta aagatgaatt tcgtcgccgt
    19381 gtcgacgtgt catttcgaca tcatcgacat tattcaccgc agggataatc aacactggca
    19441 caattattgc ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct
    19501 gccaacgggc actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa
    19561 ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt
    19621 gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac
    19681 aagaggccga atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg
    19741 tccgccgctg tccacagtgt catggtgaca tgctgcagat tgtggcagac gacggtttac
    19801 agattcggcg gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg
    19861 aaggcaacct gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc
    19921 catttgcccc ggcggcacgc ccgaagatga aaatcaccgg cattaaagcg cctgaattta
    19981 cccccagcca gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg
    20041 caccgggcat gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata
    20101 accgtctggc tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc
    20161 tggtttccag cccaggttcc ggtaaaacca ccctgctgac ggaaacctta atgcgcctga
    20221 aagacagcgt tccgtgcgcg gttattgaag gtgaccagca aaccgtgaac gatgccgcac
    20281 gcattcgcgc taccggcaca ccagcgattc aggtgaacac cggtaaaggc tgccatcttg
    20341 acgcacagat gattgccgac gccgcaccgc gtctgccact ggacgataac ggtattctgt
    20401 ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca
    20461 aagtggcggt gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcatatgt
    20521 ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct gttgccgtat ctcaactttg
    20581 acgttgagaa gtgcctcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta
    20641 tttccgccac cagcggtgaa gggatggacc agtggctgaa ctggctggag acacagcgat
    20701 gtgcataggc gttcccggcc agatccgcac cattgacggc aaccaggcga aagtcgacgt
    20761 ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca
    20821 gccgcgcgtg ggccagtggg tactggttca cgttggcttt gccatgagcg taattaatga
    20881 agccgaagca cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccggatgt
    20941 cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg
    21001 gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc
    21061 gccgaacgcc ctctgcggat tatggaagtg tgtggcggtc atacccacgc tatctttaaa
    21121 ttcggcctcg accagttact gccggaaaac gttgagttta tccacggtcc gggttgtccg
    21181 gtgtgtgtac tgccgatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa
    21241 gtcatcttct gtacctttgg cgacgccatg cgcgtaccag ggaaacaggg atcgctgttg
    21301 caggcaaaag cacgcggtgc cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa
    21361 ctggcgcagg agaatccaac ccgtaaagtc gtattcttcg gcttaggttt tgaaaccacc
    21421 atgccgacca ccgccatcac cctgcaacag gcgaaagccc gcgatgtgca gaatttttac
    21481 ttcttctgcc agcatattac gcttatcccg acgctgcgca gtttgctgga acagccggat
    21541 aacggtatcg acgcgttcct cgcgccgggc cacgttagta tggttatcgg cactgatgcc
    21601 tataatttta tcgccagcga ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt
    21661 gatctactac aaggcgtggt catgctggtg gagcagaaaa tagcggccca cagcaaggta
    21721 gagaatcagt atcgtcgagt ggtaccggat gccggtaacc tgctggcgca acaggcgatt
    21781 gccgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct
    21841 tctggcgtac acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg
    21901 gcaccgcagc aggtctgcga tgacccgcgc gcacgttgtg gtgaagtatt aacgggcaaa
    21961 tgtaagccgc atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcatttggt
    22021 gcactgatgg tctcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag
    22081 agtgaagcgt gaataatatc caactcgccc acggcagcgg cggccaggcg atgcagcaat
    22141 taatcaacag cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc
    22201 aggcacgtct tgatctggcg cagctggtag cggaaggcga ccgtctggcg ttctccaccg
    22261 acagttacgt cattgacccg ctgttctttc ctggcggtaa tatcggcaag ctggcgattt
    22321 gcggcactgc gaatgacgtt gcggtcagtg gcgctattcc gcgctatctc tcctgtggct
    22381 ttatcctcga agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag
    22441 aaaccgcgcg cacggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg
    22501 gcgcggcaga taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc
    22561 attggggcgc acagacgcta accgcaggcg atgtgttgct ggtgagcggc acgctcggcg
    22621 accacggggc gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca
    22681 gcgactgcgc ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag
    22741 cgctgcgtga tgccacccgt ggtggtgtaa atgcggtggt tcatgagttc gcggcagcct
    22801 gcggttgcgg tattgaaatt tctgaatcag cgctgccggt taaacctgcc gtgcgcggcg
    22861 tttgcgaatt gctgggactg gacgccctga actttgccaa cgaaggcaaa ctggtgatcg
    22921 ccgttgaacg caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggga
    22981 aagacgcggc gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt
    23041 atggcgtgaa acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat
    23101 aaaattctaa atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc
    23161 tggaagaaca aaatgtcata tacaccgatg agtgatctcg gacagcaagg gttgttcgac
    23221 atcactcgga cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa
    23281 ctggtaaagc gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact
    23341 caacgtgcgt cttattacgc atcgcgtgaa aaagacaccc ccattaaata tgaagacgaa
    23401 actgttctgg cacacggtcc ggtacgcagc attttgtcgc gccctgatac gctgcattgc
    23461 agttacgaag aattttgtga aacctggccg cagctggccg caggtgggct atacccaaaa
    23521 tttggtcact attgcctgat gccactggcg gcagaagggc atatttttgg tggctgtgaa
    23581 tttattcgtt atgacgatcg cccctggagc gaaaaagagt tcaatcgtct gcaaacattt
    23641 acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac
    23701 tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat taccaacgcg
    23761 gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac
    23821 tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa caaactcaac
    23881 atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag cgaagtcgat
    23941 gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc
    24001 cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacacctg gggcaaccag
    24061 attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa
    24121 ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttact gcgccagatt
    24181 gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg
    24241 aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt
    24301 gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa
    24361 atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag
    24421 ctgattgccc gtgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg
    24481 aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg
    24541 gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga taaaagctcc
    24601 ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta
    24661 ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtgcgt
    24721 ctaatcgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc
    24781 gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg
    24841 gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc
    24901 aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt
    24961 aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg
    25021 ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc tgccgcaacg
    25081 gttgtcgccc aggagggcga agatgaatat cagttgattg ttcgcgtgct gaaagaaacc
    25141 aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc
    25201 ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt
    25261 gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc
    25321 cggcagttct ggcaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg
    25381 acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc
    25441 aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca
    25501 tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga
    25561 cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg
    25621 agagatacgc ttgccggaca tgctgcctcc acctcattaa gatgtattta tattacatct
    25681 taatcttaaa gggcactatg actctaaaga agaaaggttc gccaaccgac gcaattttgc
    25741 gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc ggaaccagca
    25801 tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    25861 tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca
    25921 taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct
    25981 caagctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga
    26041 actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg
    26101 cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg cggtggaaaa
    26161 ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac aaattggcga
    26221 tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac
    26281 catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg ctatctggca
    26341 ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt ttcgcctgag
    26401 cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc cggcggaact
    26461 gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg gcctgcgccg
    26521 tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg
    26581 cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg
    26641 ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata ttcgttctat
    26701 caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc gtaacctgga
    26761 aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc tcgactgcac
    26821 cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    26881 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt
    26941 gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc tggctttacg
    27001 aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac tgccggagct
    27061 gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg agaagatggg
    27121 cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac cgccggtgct
    27181 ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg agtggcgcgc
    27241 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    27301 cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca ttcaaatcag
    27361 ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc tgaaaaacgc
    27421 cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg
    27481 caaagcactg gctctggaaa aacagcttta tgaagaactg ttcgacctgc tgttgccgca
    27541 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggtgaacct
    27601 ggcggaacgg gcctataccc tgaactacac ctgcccgacc tttattgata aaccgggcat
    27661 tcgcattacc gaaggccgcc atccggtggt tgaacaggtg ctgaatgagc cgtttatcgc
    27721 caacccgctg aatctgtcgc cgcagcgtcg catgttgatc atcaccggtc cgaacatggg
    27781 cggtaaaagt acctatatgc gccagaccgc gttgattgcg ctaatggcct atatcggcag
    27841 ctacgtaccg gcgcaaaaag tcgagattgg cccgattgac cgtatcttta cccgcgtagg
    27901 cgcggcagat gacctggctt ccgggcgttc gacctttatg gtggagatga ccgaaaccgc
    27961 caatatttta cataatgcca ccgaatatag tctggtattg atggatgaga tcgggcgtgg
    28021 aacgtccact tacgatggtc tgtcgctggc gtgggcgtgc gcggaaaatc tggcgaataa
    28081 gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacacagt taccggagaa
    28141 aatggaaggc gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt
    28201 tatgcacagc gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct
    28261 ggccggcgtg ccaaaagagg ttattaagcg cgcacggcaa aagctgcgtg agctggaaag
    28321 catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt tgctgtccgt
    28381 accagaagaa atttcgcctg cggtcgaggc actggaaaac ctggacccgg attcactgac
    28441 tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat aaaaattccc
    28501 gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa aaataccctg
    28561 tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat
    28621 caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt
    28681 attacaatcc cgcttacatc aactctcttt tttccccgaa accgacttac ttatttccac
    28741 aggcgataat attgatcgtg ggccggagag tcttgacgtc ctgcgcctgc taaaccagcc
    28801 ctggtttatc tccgttaaag gcaaccacga agcaatggcg ctggatgcgt tcgcgaccgg
    28861 cgatggcaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    28921 gcaaaaagaa gctacagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    28981 cactaacgac aacataaaat atgtcatcgc acatgcagat tatccgggga gtgaatatct
    29041 ctttggtaaa gaaatagcgg agagcgaatt actctggcct gttgatcgtg tgcagaaatc
    29101 gcttaatggc gagttacaac aaataaacgg cgctgattat tttatatttg gacatatgat
    29161 gtttgataac attcagacgt tcgctaacca gatttatatt gataccggat cgccgaaaag
    29221 tgggcggctg tcattttata aaataaggta gtctcatgct tcttctgtga agcatgagta
    29281 accagatgtt attgcaggcc attattcatt tttcgctacc agcaaagaga gatcctgctt
    29341 cgccagcgcg cgactggcac tctccggcaa accgtcgtct gtaataatct gatcaaactc
    29401 gcttaatggt aacgccagcc atgtcgccac ctgaccatat ttcgtcgcat cacagaccaa
    29461 aactcgctgg cggctggcac tggcaatcgc ccgtttcacc gtgactttat cttccgctgg
    29521 cgtagaaatc ccccgcacac tccatgacga tgcagaaata aaagcctgat caatcatcag
    29581 gctgcgcagc atggtcgcag cggcttcccc gacacaggaa cggttttccc gacacactgc
    29641 accgccagtg tgaataattg tgcaattact gttgtcgagc aagtagttcg caataacgaa
    29701 atcgtttgtg accacagtca gtgactccat gtgaatcaga tgctgtgcta tcgctaacgt
    29761 ggtcgttccc gcatccagat agatacaact tcccggctga acaagacttg ccgccagctt
    29821 gccaatagcc gctttttgcg tcattgccag cgcagttttt accagatgag aaggttcatg
    29881 cgccacgcgt cccggagact ggacgcctcc ggacaccagc acaacggctc cctgctgctc
    29941 cagtttttgt aaatcccgac gaatggtcat atgtgacaca ttcattctgt ccgttagttc
    30001 agcaatactg acaatgcctt tttcagctac catctcaagg atgatttggc gacgctctac
    30061 gggtatcaac ttttgctcct tcctttgtcc tgctgacatt ctacgctatt tgcctgcgaa
    30121 acgtgcgcgg cgcaactaac gcttagttca cataaaataa cacacaatgt taatttatgt
    30181 gaatcagctc accatatcgt tatcttccag cgcttatatt cacaatatca aacaaaatat
    30241 cacttaaatt aacaaggaga gcagatgaaa acgggatctg agtttcatgt cggtatcgtt
    30301 ggcttagggt caatgggaat gggagcagca ctgtcatgtg tccgcgcagg tctttctacc
    30361 tggggcgcag acctgaacag caatgcctgc gctacgttga aagaggcagg tgcttgcggg
    30421 gtttctgata acgccgcgac gtttgccgaa aaactggacg ctctgctggt gctggtggtc
    30481 aatgcggccc aggttaaaca ggtgctgttt ggtgaaacag gcgttgcaca acatctgaaa
    30541 cccggtacgg cagtaatggt ttcttccact atcgctagtg ctgatgcgca agaaattgct
    30601 accgctctgg ctggattcga tctggaaatg ctggatgcgc cagtttctgg tggtgcagta
    30661 aaagccgcta acggtgaaat gactgtcatg gcctccggta gcgatattgc ctttgaacga
    30721 ctggcacccg tgctggaagc cgttgccgga aaagtttatc gcataggtgc agaaccggga
    30781 ctaggttcga ccgtaaaaat tattcaccag ttgttagcgg gcgtacatat tgctgccgga
    30841 gccgaagcga tggcacttgc agcccgtgcg gggatcccgc tggatgtgat gtatgacgtc
    30901 gtgaccaatg ccgccggaaa ttcctggatg ttcgaaaacc ggatgcgtca tgtggtggat
    30961 ggcgattaca ccccgcattc agccgtcgat atttttgtta aggatcttgg tctggttgcc
    31021 gatacagcca aagccctgca cttcccgctg ccattggcct caacagcatt gaatatgttc
    31081 accagcgcca gtaacgcagg ttacgggaaa gaagacgata gcgcagttat caagattttc
    31141 tctggcatca ctctaccggg agcgaaatca tgatcaagat tggcgttatc gccgatgatt
    31201 ttaccggcgc gacggatatc gccagttttc tggtggaaaa cggtctacca acggtacaaa
    31261 ttaacggtgt tccaacaggt aaaatgccgg aagcaatcga cgcactggtg atcagcctga
    31321 aaacgcgctc ctgtccggtg gttgaagcca cacagcaatc gctggcggct ctgagctggt
    31381 tgcaacagca aggttgcaaa cagatctatt tcaaatactg ctctactttc gacagtacgg
    31441 cgaaaggtaa tatcggcccg gttaccgatg cattaatgga tgctctcgac acgccgttta
    31501 cggtcttctc tccggccctg ccggtcaacg gacgtacggt ttatcagggg tatttgttcg
    31561 taatgaatca actgctggcc gaatccggga tgcgccatca cccggtaaat cccatgaccg
    31621 acagctatct tccccgtctg gttgaagcgc aatccacagg gcgctgcggc gtcgtttcgg
    31681 cacatgtttt cgaacaaggt gtggatgccg ttcgtcaaga gctggctcgc ttacagcaag
    31741 agggctaccg ctacgcggtg cttgatgcgc tgaccgaaca ccatctggaa attcagggag
    31801 aagccttgcg cgatgcccca ctggtaacgg gcggttctgg tctggcgatt ggcctggccc
    31861 ggcagtgggc gcaagaaaac ggtaaccagg ctcgcgaagc agggcatccg ctcgctgggc
    31921 gcggcgtagt gctctccggt tcatgctctc aaatgaccaa ccgccaggtg gcacattacc
    31981 gtcaaattgc accagcccgt gaagttgatg tggcacgctg cctctcaacc gaaactctgg
    32041 ccgcttatgc acacgaactg gcagagtggg ttctgggcca ggaaagtgta cttgctccac
    32101 tggtttttgc caccgccagc actgacgcat tggcagcaat tcaacagcaa tacggtgcac
    32161 aaaaagccag tcaggcagta gaaactctat tttctcaact agcggcgcgg ttagcagcgg
    32221 aaggcgtgac acgctttatt gtcgcaggcg gtgagacctc cggcgtagtc acacagagcc
    32281 tgggcataaa agggtttcat attggcccaa ccatttcccc cggcgtgccg tgggtaaacg
    32341 cactggataa gcctgtctca ctcgccctta aatctggcaa cttcggtgat gaagcctttt
    32401 tttcacgagc ccaaagagag tttttatcat gagcgatttc gcaaaagtag agcagtcttt
    32461 gcgagaggag atgacgcgga ttgccagttc attctttcag cgcggctatg caaccggttc
    32521 ggctggcaat ctgtcgctgc ttttacctga cgggaattta ctggcgacac cgacaggttc
    32581 atgcctgggc aatctcgatc cgcagcggct ttccaaagtc gccgcggatg gcgaatggtt
    32641 aagtggtgac aaaccctcga aagaggtgct ctttcatctg gcgctgtatc gcaacaatcc
    32701 gcgctgtaaa gcggtggtgc atttgcacag cacatggtcg acggcgcttt cctgcctgca
    32761 agggctggac agcagcaacg ttattcgtcc gttcacacca tacgtggtga tgcggatggg
    32821 aaatgtcccg ctggtgcctt attaccgacc gggcgataaa cgcatcgcac aggatctggc
    32881 ggaactggca gcagacaatc aggctttttt actggcaaat catggcccag tggtttgcgg
    32941 tgaaagcctg caagaagccg ccaacaatat ggaagagctg gaggaaacgg caaagctgat
    33001 ttttattctc ggtgaccgcc cgatccgtta tctgaccgca ggtgaaattg cggaattaag
    33061 gagttaatgc aatgcctcgt tttgcagcta atttatccat gatgttcacc gaagtgcctt
    33121 ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga tgctgtggaa tttctgtttc
    33181 cctatgacta ctccaccctg caaatccaaa agcaactgga gcaaaaccat ctgacactgg
    33241 cgctgttcaa taccgctccc ggagatatta atgcggggga atggggatta tccgcccttc
    33301 ccggacgtga gcacgaagca cacgcagaca tcgacctggc actggaatat gcattggcgc
    33361 tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc cgctggcgaa gacgctgaac
    33421 ggtaccgggc agtatttatc gataatctcc gctacgctgc cgaccgcttt gcgctacacg
    33481 gcaagcgaat attagttgaa gcactcagcc ccgacgttaa gcctcattat ctcttctcca
    33541 gccagtatca ggcactggct attgttgaag aggttgcgcg agataacgtg tttattcaac
    33601 tagatacttt tcatgcgcaa aaagtagatg gcaacctgac gcatttaatc cgcgactacg
    33661 ctgggaaata tgcgcacgta caaattgccg gactacctga tcgacatgaa ccggacgatg
    33721 gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat cagggctgga
    33781 tcggttgtga atataaacct cgtggcctca ccgaagaagg gcttggctgg tttgacgcct
    33841 ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca tctacggatg
    33901 gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac attgttatgc
    33961 attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa ggtacaacca
    34021 ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat accggcgggc
    34081 gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt cactattatt
    34141 attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc agagtcactg
    34201 gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct gactctggca
    34261 gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct ttattattct tgcgccaatc
    34321 atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct gcctgtcgct
    34381 ggaatcatgc taactgttca tgtggcggta ccgccgcatc caggccctgt cgccgcagcg
    34441 gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat ttctattccc
    34501 gtagggattg ttggctactt tgcagcgaaa ataatcaata cgcgccaata tgcgatgtcg
    34561 gtggaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc aacaaaatta
    34621 agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt tattcctatc
    34681 gcgattatca tggcgggtac ggtttccgca acactgatgc cgccttcgca tcccctgctt
    34741 ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct ggtgctggca
    34801 ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga cattatgggc
    34861 tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg ggtatttggc
    34921 aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca atatgctaca aatgattgac
    34981 ctgcctctgt taccagccgc atttattatt tcactggctc tgcgtgcatc gcaggggtca
    35041 gcaaccgtag caatcctgac aaccggcggg ttactctcag aagcggtgat gggattgaat
    35101 ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg cgcgtcacat
    35161 attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt agcagacggt
    35221 ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt aattacctgg
    35281 tgcgtatggg cggtaatttg accttaataa aaaggtccga tgggcatcgg accttttatt
    35341 taacgcttac cttccaccag cagcggcggg atgctcggca ccattggcgc gtcatcaata
    35401 tctttctgcg tcatgcggaa cgcttcggga taatgttcgc ggctggtacg gcgcagcggt
    35461 tcggtatcgc gccaggtata aaggcaatgc tggcactgat ataccgtcca gacatctttc
    35521 accggcgatt tcgccatcac ttcaatctgt tcatcggcac aacgtggaca aatcatcttc
    35581 ttctccttat ttacgtgcag ccagcatagc ggtcagtttt tcagcccagg ctttggtttc
    35641 cggtaaatcc accaccggtt ggctgtagtg accacggttg tccggggcga caggcgtagt
    35701 ggcgtcgata atcagcttgt cggtgatccc cgcagggctt gagcctggat cgagttccag
    35761 cacggacata ttcggcaact gcaccaaatc ccctgccggg ttcactttcg aggagagcgc
    35821 ccacatcacc tgcggcaggt tgaacgggtc aacgtcttca tcgaccataa tcaccatctt
    35881 cacgtagccc agaccatgcg gcgtggtcat tgcgcgcagg cccaccgcgc gggcaaagcc
    35941 gccgtagcgt tttttggtgg agataatcgc cagcaggcca tgggtgtaca tggcgtttac
    36001 cgcctgcact tcagggaact cggctttcag ctgctgatac agcggcacgc aggtggctgg
    36061 ccccatcagg tagtcgattt cggtccacgg cataccgagg tacagcgatt cgaaaatcgg
    36121 cctggtgcgg taagagactt tatcgatgcg caccacggtc atgttacgcc cgccggagta
    36181 gtgcccggta aactcaccga acggcccttc gatttcgcgt ttacggcttt cgataacccc
    36241 ttcgaggatc acttctgaac cccacggcac atcaaaaccg gtcagcgggg cggtggcgat
    36301 cgggtacggg ctttcacgca gcgcgcctgc catttcgtac tcggactgat cgtatttcag
    36361 cggcgtggcc cccatcagcg tgatgatcgg atcgttaccg agcgtgatcg caatcggcag
    36421 atcttcaccg cgctcttctg ctttatgcag atgcagggcg atatcgtgca tcggcaccgg
    36481 ttgcaggccg agcttacgct tgcccttcac ttccatgcgg tagatgccga cgttctgctt
    36541 gccgaagtta tctgggtcga gcggatcgcg ggaaaccacg caggctttgt cgagatagaa
    36601 accgccatcg ccatcgttta aacgaaacag cggcaggata tcgaacaggt taatctcttc
    36661 gccatcgacg gtgttctgcg cccaggctgg attggcgcgg cgctccgggg caatcgggaa
    36721 gttatcccag cggcggataa actcatcaat ctgctttttg accggggtgt ttggtggcag
    36781 gccaagggaa atcgcgtggt tctgccagga accgatggta ttcatcgtca cgcgggcatc
    36841 ggtaaagccg cgaatattat caaaccacag cgcgggcgcg ccgtcgccga tacgcccggt
    36901 ggcgttagct gctgctgcca gatccggctc agcgttcacc tcttcgctga ttttcagtaa
    36961 ctggccgtgg tcatcaagcg cctgtaaaaa gctgcgtaaa tcatcaaatg ccattattca
    37021 ttctcctgag aaaaattccg ggcctgcggc aatccttgcc agcgcctggc atggggatgt
    37081 tcgaggccaa atttatccag cacgcgggct accacatggt ggacaatgtc atctaccgtt
    37141 tcgggatggt tataaaaggc aggcatcggc ggcaccatcg ccacgcccat gcgtgaaagt
    37201 gcgagcatat tttcgagatg gatggtgcta agcggcattt cacgcggcac cagcaccagt
    37261 ttgcggcctt ctttgagcac gacgtccgcc gcgcgcccta ccaggccatc ggcgtaacca
    37321 gcgcggatac cggcgagcgt tttcatactg cacggaataa cgatcatgcc gtcggtacga
    37381 aaagaaccgg atgagatgat cgccgcctga tccgccgggt tatggctgaa gtctgcgagg
    37441 gcagcaacat cgcgagcgct gtaaggcgtt tccagttcaa tggtggtttt cgcccacttc
    37501 gacatcacca gatgagtctc gacattcggc atctcccgca gcgcttgcag taatgccaca
    37561 ccaagaggcg caccggtagc ccctgtcatc ccgacgatca gtttcattgc ttatcctctt
    37621 aattgttcgt atacgaacgt tattattaac atactccttc atttccacag cggcaaggat
    37681 tgaaaagtaa caacatcttc ttcgtcacta aaaaaccgct atgatagcga cagagaatta
    37741 gcccggagtt tacatggcgt tacgaaataa agcgttccat cagttacgac agctttttca
    37801 gcagcacacg gctcgctggc agcacgagtt acctgacctg accaaaccac agtacgcggt
    37861 gatgcgcgcc attgctgata aacctggtat cgaacaggtg gcactgatag aagcagctgt
    37921 cagcaccaaa gcaacgttag cagaaatgtt ggcaagaatg gaaaatcgtg gcttagtcag
    37981 acgtgaacat gaccccgctg ataagcggcg acgctttgtc tggttgactg ccgaaggcga
    38041 aaagatactt gctgcggcga tacccattgg tgacagcgtg gatgaggaat ttttggggcg
    38101 tttgagtgga gaagagcagg agctgtttat gcagctggtg cgcaagatga tgaacgcata
    38161 gggggcaaat ttccggatgc gacgctatcg cgtcttatcc agactacaaa tacaccgact
    38221 caccaaccga aagaaaaaag gccagcctcg cttgagactg gcctttctga cagatgctta
    38281 cttactcgcg gaacagcgct tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac
    38341 ggcgcaggcc ttcaacctga atctggcgaa cacgttcacg ggtgaggcca atttcacgac
    38401 ctacatcttc cagtgttgcc gcttcgtacc ccagcaaacc gaatcgacgt gccagcactt
    38461 cacgctgttt ggcgttcagc tcgaacagcc atttgacgat gctctgcttc atatcatcat
    38521 cttgcgtggt atcttccgga ccgttctctt tttcatcggc caggatgtcc agcaacgctt
    38581 tttcggaatc accacccagc ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca
    38641 tacggctgac gtcatcaact ggcttatcca gttgctctgc gatctcttcc gcacttggtt
    38701 catggtccag cttatgggac aactcacgtg cggttcgcag gtaaacgttc agctccttta
    38761 cgatgtgaat cggcaaacga atagtacggg tttggttcat aatcgcccgt tcaatcgtct
    38821 ggcgaatcca ccaggttgcg tatgtcgaga agcggaaacc acgttccggg tcaaacttct
    38881 ctaccgcgcg gatcagcccc aggttgccct cttcgataag gtccagcaac gccagaccac
    38941 gattgccata acggcgggca atttttacca ccagacgcaa gttactctcg atcatccggc
    39001 ggcgagaggc gacatctcca cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg
    39061 ttaacagtgg tgaataacca atctcaccaa ggtaaagctg agtcgcgtcc aacacacgct
    39121 gtgtggctcc ctgcgataac agttcctctt cggccaaatc gttatcactg ggttcctctt
    39181 ctactaaggc cttttcgtca aaaacctcaa ctccgttctc atcaaattcc gcatcttcat
    39241 ttaaatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc gtgatccctt
    39301 gacggaacat tcaagcaaaa gcatggttcc gccgatttat cgctgcggca aataacgcag
    39361 cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc
    39421 ggtgctaccc atggttgcta ttttttgccc cgccttcact tcttgttgtt cccggaccag
    39481 cattgtgtcg ttatgggcgt aggcactcag gtaatcatca ttatgtttga tgataatcag
    39541 attaccgtag ccgcgcagcg cgttaccagc ataaacaacg cggccatctg cggtcgcgat
    39601 aattgcctgt cctttgctgc ctgcgatatc aatccccttg ttgcccccct cagaagcgcc
    39661 aaaggtttcg atcactttgc cctcagtcgg ccagcgccag gtggagatag gcgtactggt
    39721 tgatgtactg ctgacagttg gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt
    39781 gaccgtggtc gcagctggct tgttgttcgg caacattttg ttagcactct gttcacccga
    39841 agattcagaa tacgtaattg tcggttgcga cgcaacagca acggtggaat tttgtgcagg
    39901 cttgatcaca actccttgct ctgctgcgtc ggcctgggta atggcatttc cgccagtgat
    39961 tggcgtaccg gaagcattac ccacctgcaa ggtctgacca acgttcagcg cgtatggtgc
    40021 ctgaatattg ttgcgctgag caaggtcacg gaaatcgttg ccagtaatcc aggcgatata
    40081 gaaaagtgtg tcgccttttt tcacggtata ggtactgccg ctataactgc ctttcggaat
    40141 gttcccatac tgacggttat agacgatgcg tccgttttcc atctgtaccg gctgctgagc
    40201 tactggctgc actggctgga tttgcggttg ttgagtagcc tgaatttgtg gctgctgtac
    40261 cggctgaatt tgcggttgct gggctgtaga cgtcgtcccc attttcggcg gcggcgtaat
    40321 caacatacca gaattggtat ttgcaggcgc attgccatta acggagctga ccggtgccgg
    40381 tggatttgaa gtgtcagaac agcctgccag ccatagcgaa accagtgaca aagccgcaat
    40441 gcggcgaacg gtgaattttg ggcttcccgc gctcatttat cccccaggaa aaattggtta
    40501 ataaccagtg acataattac cgtgcaaggc accctactga acactggaaa agatgttcac
    40561 gatacgctga cctgcggcaa aataaccagg aaaaatccag gtatttcctc acgttttaag
    40621 ccagctcacc cttcactaaa gggacaaagc gcacggcctc cacggtatcg ataataaatt
    40681 cgcctccccg acgacgcacc cgtttcaaat actggtgctc ctcccctacg ggtaagacga
    40741 gaatcccgcc ttcgtccagc tgcgtcatta gcgcagttgg aatttccggc ggtgccgccg
    40801 taacaatgat agcgtcaaac ggcgcgcgtg cctgccaacc ttgccatcca tcgccatgac
    40861 gggttgaaac attatgtaaa tcaagatttt tcaggcggcg acgcgcctgc cactgcaaac
    40921 ctttaatccg ttcgaccgag caaacatgct ggacaagatg cgccaggatt gccgtttgat
    40981 atcccgaacc ggtgccaatt tccagcaccc gcgactgcgg cgtcagctcg agtaattcgg
    41041 tcattcgcgc caccatatac ggctgcgaaa ttgtctgccc ctgacctatc ggcaaggcga
    41101 tattgtccca ggctttttgt tcaaacgctt catcaacgaa tttttcacgc ggcacggcag
    41161 caagtgcatt cagcacctgc tcatcctgaa taccttgcgc acgtaattga tccagaagtg
    41221 cttgtacgcg tctgcttacc attgcgtgcc aactcccacg ctgtttaacc agtctgaaac
    41281 cacatcttgc gcgctatgcg cagttaaatc cacatgcagc ggcgtgatgg agacatagcc
    41341 ctcatctacc gcagcaaaat cggtccccgg accggcatca catttaccgc ccggcgggcc
    41401 aatccagtac agcgtattgc cgcgcggatc ttgctgcggg atcacctgat ctgccggatg
    41461 tcgtgtaccg cagcgcgtca cgcgaatacc tttgatttgg tccagcggca aatccggtac
    41521 atttatatta agaatacgcc ctgttcgcag cggttctttg cacagtgcac gcagaataga
    41581 acaggtaact gccgcagcgg tgtcgtaatg tttatgcccg tctagcgaga cggcaagcgc
    41641 cggaaaacct aaatggcgac cttccatcgc ggcggctacc gtaccggaat aaataacatc
    41701 atcccccaga ttcggcccgg cgttaatccc ggacacaaca atgtccgggc gcggacgcat
    41761 cagagcattc acgccaagat agacgcaatc ggtcggggtt cccatttgca cagcaatatc
    41821 accattttca aaggtaaacg tgcgcaggga ggattccagt gtcagagaat ttgaagcgcc
    41881 gctgcggtta cgatcggggg cgaccacctg aacgtcagca aactcacgca aggctttcgc
    41941 cagcgtttgt ataccgggtg catgtacccc gtcatcatta ctcagcaata tgcgcataat
    42001 cacctgttgt gttgataagt tccctgacaa cgctggttgc aaaactaccc gccggaagcc
    42061 agaaacggat ctctacggtg acgtcatccc accaattcca gcttaattgt tgcggataca
    42121 gcagcatcgc tctgcgcgcg gcttcaactt tttcgcgcac cagtaaagct tgtaattcag
    42181 tttctgcggc gacagctgct tgttcgaatg ccagcgcttc acgctgagtt ccccattcgc
    42241 cactgcccgg caatgcggcg gttatcatca gctctttatc gttgacgcga cgctgtaatt
    42301 ccgccagttc ttcggtggtt gcgacaaacc agctaccacg tccggctaat tgtagcgcat
    42361 cgccgtcaac aacttgatta acgtctgctt ttttgaggcg ctcagcaaca atctgattaa
    42421 acaacgcact gcgggctgcc gacaaccaaa aactccgttt attgcgatcg cgcaccggag
    42481 tattggtttg cgcccagcgc tgcgccccct gcaagttgct accgccaatc ccaaaacgtt
    42541 gggcaccgaa gtagttcggt acacctttta cgcaaatatc gatcagacgt tgttcaacgt
    42601 catcgcgatt gctcacttcg cgcagaacca gggtaaaggc gttacctttc agcgcgccta
    42661 aacgcagctt gcgcttgtgc cgcgcatact ccagcacctg gcagccttcc agttgaaagg
    42721 cgctcagatc gggcatttcc ttgcctggca cgcgagcgca taaccactgt tccgtaacag
    42781 catgtttgtc tttttgccca gcgaagctga cttcacgggc atgaattttc aggaatttcg
    42841 ccagtgcatc cgccacaaaa cgggtattgc agccgttttt gaggattcta accagaatat
    42901 gctcaccttc accatcaggc tcaaagccca aatcttccac caccacaaag tcttccggat
    42961 tggctttcag cagcccggtg ccttgcggtt taccgtggag gtaagtgaga ttatcaaact
    43021 caatcatttt gttgccttaa tgagtagcgc caccgcttca caggcaatcc cttccccacg
    43081 tccggtaaat ccaagttttt ccgtagtagt ggctttcacg ttaacatcat ccatatggca
    43141 gccgagatct tcggcaataa acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc
    43201 ctgagcgatg atagtgacat cgacgttgcc cagggtataa cccttcgcct gaatacgacg
    43261 ccaggcttcg cgtagcagct cgcggctgtc ggcaccttta aatgccggat cggtatccgg
    43321 gaacagcttg ccgatatccc ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg
    43381 gagcgccacg tcgccatcag aatgcgccag caatcctttt tcgtaaggaa tgcgtacgcc
    43441 accaatgata attgggcctt caccgccaaa ggcatgtacg tcaaaaccgt gtccaattcg
    43501 cattatgtat tctcctgatg gatggttcgg gtgaggtaaa actcggccag tgccaaatct
    43561 tccgggcgcg tgactttaat gttatccgca cggccttcga ccaactgagg atggaatccg
    43621 caatattcca gcgccgaggc ttcgtcggta atagtcgcgc cttcatttag agcgcgcgtc
    43681 agacagtcat gtaacagctc acgagggaaa aattgcggcg tcagcgcgtg ccataagccg
    43741 ttgcgatcaa cggtatgagc aatggcattt ttgcccggtt cggcacgttt catcgtatcg
    43801 cgcactggtg cggctaggat ccctcccgtg cggctggttt cgctcaacgc caacaatcgc
    43861 gcgaggtcat cctgatgcag acaaggacga gcggcgtcat gcaccaatac ccactgcgcg
    43921 tcgccagcgg ctttcaaacc tgccagcacg gaatcggcac gctcatcacc gccatctaca
    43981 acggtgattt gcggatgatt cgccagagga agttgtgcaa aacggctatc gccaggactt
    44041 atggcaatga cgacacgttt cacccgggga tgcgccagca gcgcatgcac cgagtgttca
    44101 agaatggttt gattaccgat tgagagatat tgcttaggac attccgtttg cattcgacgg
    44161 ccaaatccgg ccgccggaac cacggcgcaa acatccaaat gagtggttgc catgttaatt
    44221 cccgggctga tttatcgatt gttttgcccc gcagactgtg cgcgcttcga cgcgtcaggc
    44281 accagacgat aaaaagtttc gcccggcctg gtcatgctga gttcattacg cgcacgctct
    44341 tcgagcgcct cctggccgcc attgagatcg tcaatttcgg caaaaagttg atcgtttcgc
    44401 gctttaagtt tcgcgtttgt agcttgctgt gccgccacat catcattgac gcgggtatag
    44461 tcatgtatac cgttcttacc gaaccacagc gaatactgta gccagaccag aatagccagc
    44521 aacagcagcg ttagtttacc catcctgccc cctgaaaaac ggcatcatca tcccatgcat
    44581 ccgaagacga ctctacatcc tctgttgggg ataccgcgac aacgcgggca aatgtaccac
    44641 atttgtccat tgttacgtat acccagggcg tgcagaacat aatctcatta ttagttacgg
    44701 tttgaattat gaacagagga gacgggaaag tacaaattag cccagtagcc acataaacag
    44761 tgcgccaaac ataatgccta ctgtcaccag ggtgaaaaca atactgtagc gtagctttcc
    44821 gtccatcaat gaatgcagcg caatccccac cactaccgcg acgggcatca gcgccagaaa
    44881 gaaaggccag gtgtagataa agaagaacag cgtgttagag ccataaatca acatcggcat
    44941 cgccagcgca aataaccagg agataaaacc gaccacggca ccaggcagtg accatgtggt
    45001 ttcttcatcc tcagtaaggc tgtcgttatt tgttagtgta atgttatggc tattacgcat
    45061 atttgatcct gttactttga cgaaccgggc atggaaaccc ggtggtgtct caggatctga
    45121 taatatcgtt ctgtctcaac agatctaata attgctgtac caaatttgtt actaattgtt
    45181 caccattgag atgaatttct gccgattcag gcgcttcgta aacagaatct attcccgtaa
    45241 agttgcgcag ttcaccggca cgcgctttct tatataagcc ttttggatcg cgggcttcgc
    45301 aaatcgccag cggcgtatcg acaaacactt cgataaagcg cccttctcct acgcgttcgc
    45361 gaaccatctg gcgttcggcg cggtgtggcg agataaatgc ggtcagcact accagtccgg
    45421 cttcaaccat caaattcgcc acttcaccga cgcgacggat attttcttta cgatcggcat
    45481 cgctaaaacc gagatcgctg cataatccgt ggcgaacatt atcgccatcc agcagatacg
    45541 tactgacgcc gagtttatgt aacgcctcct ccagcgcccc ggcgaccgtt gatttaccgg
    45601 acccggagag gccggtaaac cacagcacta caccacgatg accgtggtgt agctcgcgtt
    45661 gttgcacagt gaccggatgg ctatgccaga cgacgttttc gtcatgcagc gccattattt
    45721 atcccccagc aaatcgcgcg cgccccagtg cggaaagtgg cgacgaacca gagcattcaa
    45781 ttccagttcg aatgcactga attcagatgg cgcagcagtt gcctggctaa ctggctcgtg
    45841 caccataccg gcacccacgg tcacattgct caggcgatcg ataaaaatca gcccacccgt
    45901 caccggattt tgttgataac gatctaacac cagcggctcg tcaaaagtga gatccacgag
    45961 gccgatccca ttcagtggca ggttttcaac ttcacgctgg gtaaggttat taatatcaac
    46021 ctgatagcga atgccatcaa cacgcgcgcg cgtcttctta ccggcaattt tgatgtcgta
    46081 actctgccct ggagaaagcg gctgttccgc catccatacc acatccaccg acgcgctctg
    46141 caccgccggt aacgcttcgt ctgccgccag cagcagatcg ccacggctga tgtcgatctc
    46201 atccgtcagc accagggtga tcgcttctcc ggcaaaggct tcttcgcgat caccatcaaa
    46261 agtcacgatc cgcgcgacgt ttgattccac accagaaggc agcactttta cacgttgccc
    46321 gacttccacg cgaccggatg ccagcgttcc ggcgtaacca cgaaaatcga gattcgggcg
    46381 attaacgtac tgcaccggga agcgcattgg ctgagcatcc accactcgct ggatctccac
    46441 ggtttccagc acttcgagca gtgtcggacc gctgtaccac ggcatacttt cactttgcga
    46501 agccacgttg tcgccttcca gcgcagagag cggcacaaag cgaatatcca gattgccagg
    46561 taactgttcg gcgaaagtca ggtagtcttt acgaatgcgg gtgaatgtct cttcactgta
    46621 atccaccaga tccattttgt tgatcgctac gaccagatgt ttgatcccca gcagcgtgga
    46681 gataaaactg tgacgacggg tttgatcgag cacgccttta cgggcatcga tcagtaagat
    46741 cgccagctca cacgttgagg cgccggtcgc catattgcgg gtatactgct cgtgcccggg
    46801 ggtgtcggcg ataataaatt tacgcttctc ggtagagaaa tagcggtagg ccacatcaat
    46861 agtgatgccc tgttcgcgct cagcttgcag gccgtccacc agcagagcca gatccagttt
    46921 ttcgccctgg gtgccgtgac gcttactgtc gttatgcagc gatgagagct gatcttcgta
    46981 gatttggcgg gtatcgtgca gcaggcgacc aatcagggta cttttgccgt catcgacgct
    47041 accacaggtc agaaaacgca gcaggctttt atgttgttgc gcaatcatcc aggcttcgac
    47101 gccgccttca ttggcgattt gttgtgcaag tgcggtgttc atcttaaaaa tacccctgac
    47161 gttttttcag ctccatagac cccgcctggt cgcggtcaat cacgcggccc tgacgttcac
    47221 tggtggtaga aaccagcatc tcttcgatga tctccggcag tgtttgtgca tttgactcca
    47281 ccgcaccggt cagcggccag cagcccagcg tacggaaacg caccatccgt tttttaatca
    47341 cttcgcccgg ttgcaggtcg atacggttgt catcaatcat catcaacata ccgtcgcgtt
    47401 ccagaaccgg acgttccgca gcgagataca gcggcacaat gtcgatattt tccagccaga
    47461 tgtattgcca gatatcctgc tcggtccagt tagagagcgg gaaaacgcgg atgctttcgc
    47521 ctttgttaat ctgcccgtta tagttgtgcc acagctccgg gcgctgattt ttcgggtccc
    47581 agcgatggaa gcgatcacgg aaagagtaga tacgctcttt agcacgggat ttctcttcgt
    47641 cgcggcgcgc accaccgaag gcggcatcaa aaccgtattt attcagcgcc tgcttcaggc
    47701 cttcggtctt cataatatcg gtatgtttcg cgctgccgtg cacgaatgga ttaatcccca
    47761 tcgccacgcc ttccgggttt ttatgcacca gcagctcgca gccgtaggct ttagcagtac
    47821 gatcgcggaa ctcatacatc tcgcggaatt tccagccggt atcgacatgc agcaacggga
    47881 aaggcagcgt acctggataa aacgccttgc gcgccagatg cagcatgacg ctggaatctt
    47941 taccgataga gtagagcatc accggatttg agaattctgc cgccacctcg cgaataatgt
    48001 ggatgctttc cgcctccagt tgccgcaggt gagtaagtcg tatttgatcc ataaccgttc
    48061 ctttgcaata ccgctatttt cttgccatca gatgtttcga ctatagggag cgtaagagaa
    48121 cgaatgaaat taccaattag aatgagtagt tccttaacgg aataacgatt tggcaaagct
    48181 aatatcaaaa agtgcttaag gcaccggatt tcgggcgttt aggaagattt gaaattgttt
    48241 tagcgcagcg gcagtttcat actatggcgg taaaaaaatt tgcatggtat ttaaggactc
    48301 actatgtttt ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt atgctttatt
    48361 ctccccgtac acgcctcgtc acctaaacct ggcgattttg ctaataccca ggcacgacat
    48421 attgctactt tctttccggg acgcatgacc ggaactccag cagaaatgtt atctgccgat
    48481 tatattcgcc aacagtttca gcaaatgggt tatcgcagtg atattcggac atttaatagt
    48541 cggtatattt ataccgcccg cgataatcgc aagaactggc ataacgtgac aggaagtacg
    48601 gtgattgccg ctcatgaagg caaagcgccg cagcagatca tcattatggc gcatctggat
    48661 acttacgccc cgctgagcga tgctgacgcc gatgccaatc tcggcgggct gacgttacaa
    48721 ggaatggatg ataacgccgc aggtttaggt gtcatgctgg aattggcaga acgcctgaaa
    48781 aatacgccta ccgagtatgg tattcgattt gtggcgacca gcggcgaaga ggaagggaaa
    48841 ttaggcgctg agaatttact caagcggatg agtgacaccg aaaagaaaaa tacgctgctg
    48901 gtgattaatc tcgataactt aattgttggc gataaattgt atttcaacag cggtgtaaaa
    48961 actcctgaag cagtaaggaa attaacgcgc gacagggcgc tggcaattgc gcgcagtcac
    49021 ggaatagccg caacgaccaa tccgggtttg aataaaaatt atccgaaagg cactgggtgt
    49081 tgtaatgacg cagaaatatt cgacaaagcg ggcattgctg tactttcggt ggaagcgact
    49141 aactggaatc ttgggaataa ggatggttat cagcaacgcg caaaaacagc cgcattccct
    49201 gcgggaaata gctggcatga cgtaagactg gataatcaac agcatattga taaggcactt
    49261 cctggaagaa tagaacgtcg ctgccgtgac gttatgcgga taatgctacc tctggtgaag
    49321 gagttggcga aggcgtcttg atgggtttga aaatgggagc tgggtgttct accgcagggg
    49381 cggggaattc taagtgatat ccatcatcgc atccagtgcg cccggtttat ccccgctggc
    49441 gcggggaact cttactgctt ggtatgcgga atcacaccct gaacggttta tccccgctgg
    49501 cgcggggaac actgaagcat caaacatttg gtggaccaaa cggacggttt atccccgctg
    49561 gcgcggggaa cactgtacgc ggcgagtttt agcgacaggt catcccggtt tatccccgct
    49621 ggcgcgggga acacggatct gccagcgcct ctgcggggcg gtaaaccggt ttatccccgc
    49681 tggcgcgggg aacacactaa gcatacataa ctgttt
//
LOCUS       DAMZII010000020        48587 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZII010000020 DAMZII010000000
VERSION     DAMZII010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.020"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            121..2760
                     /locus_tag="POA77_001124"
     CDS             121..2760
                     /locus_tag="POA77_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDI6364591.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAEQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2757..2942
                     /locus_tag="POA77_001125"
     CDS             2757..2942
                     /locus_tag="POA77_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDI6364592.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2947..3276
                     /locus_tag="POA77_001126"
     CDS             2947..3276
                     /locus_tag="POA77_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDI6364593.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3448..4353)
                     /gene="feaR"
                     /locus_tag="POA77_001127"
     CDS             complement(3448..4353)
                     /gene="feaR"
                     /locus_tag="POA77_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDI6364594.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4589..6088
                     /gene="feaB"
                     /locus_tag="POA77_001128"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             4589..6088
                     /gene="feaB"
                     /locus_tag="POA77_001128"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDI6364595.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(6146..8419)
                     /gene="tynA"
                     /locus_tag="POA77_001129"
     CDS             complement(6146..8419)
                     /gene="tynA"
                     /locus_tag="POA77_001129"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDI6364596.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8667..10712)
                     /gene="paaZ"
                     /locus_tag="POA77_001130"
     CDS             complement(8667..10712)
                     /gene="paaZ"
                     /locus_tag="POA77_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDI6364597.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            10997..11926
                     /gene="paaA"
                     /locus_tag="POA77_001131"
     CDS             10997..11926
                     /gene="paaA"
                     /locus_tag="POA77_001131"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDI6364598.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            11938..12225
                     /gene="paaB"
                     /locus_tag="POA77_001132"
     CDS             11938..12225
                     /gene="paaB"
                     /locus_tag="POA77_001132"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDI6364599.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12234..12980
                     /gene="paaC"
                     /locus_tag="POA77_001133"
     CDS             12234..12980
                     /gene="paaC"
                     /locus_tag="POA77_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDI6364600.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            12995..13492
                     /gene="paaD"
                     /locus_tag="POA77_001134"
     CDS             12995..13492
                     /gene="paaD"
                     /locus_tag="POA77_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDI6364601.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13500..14570
                     /gene="paaK"
                     /locus_tag="POA77_001135"
     CDS             13500..14570
                     /gene="paaK"
                     /locus_tag="POA77_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDI6364602.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14567..15334
                     /locus_tag="POA77_001136"
     CDS             14567..15334
                     /locus_tag="POA77_001136"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDI6364603.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15334..16122
                     /gene="paaB"
                     /locus_tag="POA77_001137"
     CDS             15334..16122
                     /gene="paaB"
                     /locus_tag="POA77_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDI6364604.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16124..17551
                     /gene="paaC"
                     /locus_tag="POA77_001138"
     CDS             16124..17551
                     /gene="paaC"
                     /locus_tag="POA77_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDI6364605.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     TRLARPVVVIDKMAGKVVTIAAAAVNPDSATHKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17541..17963
                     /gene="paaI"
                     /locus_tag="POA77_001139"
     CDS             17541..17963
                     /gene="paaI"
                     /locus_tag="POA77_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDI6364606.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTVQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTIIGEA"
     gene            17963..19168
                     /gene="paaJ"
                     /locus_tag="POA77_001140"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17963..19168
                     /gene="paaJ"
                     /locus_tag="POA77_001140"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDI6364607.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19195..20508
                     /gene="paaF"
                     /locus_tag="POA77_001141"
     CDS             19195..20508
                     /gene="paaF"
                     /locus_tag="POA77_001141"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDI6364608.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20609..21559
                     /gene="paaX"
                     /locus_tag="POA77_001142"
     CDS             20609..21559
                     /gene="paaX"
                     /locus_tag="POA77_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDI6364609.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            21541..22131
                     /gene="paaY"
                     /locus_tag="POA77_001143"
     CDS             21541..22131
                     /gene="paaY"
                     /locus_tag="POA77_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDI6364610.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKLTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            22361..23221
                     /gene="pdxI"
                     /locus_tag="POA77_001144"
     CDS             22361..23221
                     /gene="pdxI"
                     /locus_tag="POA77_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDI6364611.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23285..25591
                     /locus_tag="POA77_001145"
     CDS             23285..25591
                     /locus_tag="POA77_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDI6364612.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDSYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25762..26367
                     /locus_tag="POA77_001146"
     CDS             25762..26367
                     /locus_tag="POA77_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI6364613.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECDQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNALLVILMLFCTILTEFCGLLAQTINGIRSFAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLIWTAINRCRSVLLMSAER"
     gene            26367..27263
                     /locus_tag="POA77_001147"
     CDS             26367..27263
                     /locus_tag="POA77_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6364614.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            27279..29036
                     /locus_tag="POA77_001148"
     CDS             27279..29036
                     /locus_tag="POA77_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDI6364615.1"
                     /translation="MENSRIPGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            29026..30342
                     /locus_tag="POA77_001149"
     CDS             29026..30342
                     /locus_tag="POA77_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDI6364616.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(30393..30998)
                     /gene="azoR"
                     /locus_tag="POA77_001150"
     CDS             complement(30393..30998)
                     /gene="azoR"
                     /locus_tag="POA77_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI6364617.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31199..35101
                     /gene="hrpA"
                     /locus_tag="POA77_001151"
     CDS             31199..35101
                     /gene="hrpA"
                     /locus_tag="POA77_001151"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDI6364618.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            35373..36173
                     /locus_tag="POA77_001152"
     CDS             35373..36173
                     /locus_tag="POA77_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI6364619.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36370..37809
                     /gene="aldA"
                     /locus_tag="POA77_001153"
     CDS             36370..37809
                     /gene="aldA"
                     /locus_tag="POA77_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI6364620.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37851..38852)
                     /gene="gap"
                     /locus_tag="POA77_001154"
     CDS             complement(37851..38852)
                     /gene="gap"
                     /locus_tag="POA77_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6364621.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            39041..39571
                     /gene="cybB"
                     /locus_tag="POA77_001155"
     CDS             39041..39571
                     /gene="cybB"
                     /locus_tag="POA77_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDI6364622.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39816..39989
                     /locus_tag="POA77_001156"
     CDS             39816..39989
                     /locus_tag="POA77_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364623.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(40061..40210)
                     /gene="hokB"
                     /locus_tag="POA77_001157"
     CDS             complement(40061..40210)
                     /gene="hokB"
                     /locus_tag="POA77_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDI6364624.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40609..42249
                     /gene="trg"
                     /locus_tag="POA77_001158"
     CDS             40609..42249
                     /gene="trg"
                     /locus_tag="POA77_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDI6364625.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42287..43210)
                     /locus_tag="POA77_001159"
     CDS             complement(42287..43210)
                     /locus_tag="POA77_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6364626.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            43427..44770
                     /locus_tag="POA77_001160"
     CDS             43427..44770
                     /locus_tag="POA77_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6364627.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            44995..46650
                     /gene="opgD"
                     /locus_tag="POA77_001161"
                     /gene_synonym="mdoD"
     CDS             44995..46650
                     /gene="opgD"
                     /locus_tag="POA77_001161"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDI6364628.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            46790..47014
                     /locus_tag="POA77_001162"
     CDS             46790..47014
                     /locus_tag="POA77_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDI6364629.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            47077..47613
                     /gene="rimL"
                     /locus_tag="POA77_001163"
     CDS             47077..47613
                     /gene="rimL"
                     /locus_tag="POA77_001163"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDI6364630.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDVYDDVNLYARIIDS"
     gene            complement(47608..>48587)
                     /locus_tag="POA77_001164"
     CDS             complement(47608..>48587)
                     /locus_tag="POA77_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364631.1"
                     /translation="RKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAGG
                     WIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSEI
                     SQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARVS
                     ASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKAG
                     IEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESRI
                     SGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
BASE COUNT        11989 a        11508 c        12878 g        12212 t
ORIGIN      
        1 ttgaaaaatt catcaacaac tatgcttagt gtaggcgcaa ccttcaactg aacggttaaa
       61 catgccacaa tacccgtatt gaatgcttaa tttttcgcta aatcaggata ttaactaccc
      121 atgctgggta aatataaagc cgttctcgcg ctgttattac tgattattct tgtgccgttg
      181 acgctgctga tgacgctcgg gctgtgggtt cccacgctgg cgggcatctg gctaccgctc
      241 gggacacgta ttgcattaga tgaaagccca cgcattacgc gtaaaggttt aatcattccc
      301 gatctccgtt atctggtggg agattgtcag cttgcgcata tcaccaacgc cagcctttca
      361 catcccagcc gctggttatt gaacgtcggc atggtagaac ttgattctgc ttgtctggcg
      421 aaattgccgc agacggagca atcgccagct gctccaaaaa ccctcgcgca gtggcagtcc
      481 atgctgccta acacctggat caatatcgat aaactgattt tttctccctg gcaggaatgg
      541 cagggaaaac tctctctcgc attaacctct gatatccagc aactgcgtta tcagggcgaa
      601 aaagttaaat ttcaaggcca gctgaaaggg caacaactta cagtcagcga actggatgtc
      661 gtcgcgtttg aaaatcagcc gccggttaaa ctggtggggg aatttactat gccgctcgtg
      721 ccggatggac ttcctgtaag tgggcatgct actgcgacgt taaacttgcc gcaggaaccg
      781 tcactggtgg atgcagagct ggactggcag gaaaatagcg ggcaattgat tgtgctggca
      841 cgggataacg gcgatccgtt gctcgatttg ccgtggcaaa ttactcgtca acaattgacc
      901 gtaagcgatg gtcgctggag ctggccgtat gcaggttttc ctttgagtgg ccgactgggt
      961 gtcaaagtcg acaactggca ggcagggctc gagaacgctc tggtcagcgg gcgactgagt
     1021 gtgctgaccc aggggcaagc gggtaaaggc aacgcggtgc ttaattttgg tccaggaaaa
     1081 ttaagcatgg aaaaaagtca gatgccactg caactgaccg gtgaagcgaa acaggcggac
     1141 ctcattttat atgcccgctt acccgcgcag ctaagtggaa gtctgactga cccaacgctg
     1201 gcctttgagc ccggcgcgtt attacgttcg aagggaagag tcatcgattc gctggacatc
     1261 gatgaaatcc gctggccttt agcgggtgta aaagtcaccc aacgtggtgt tgacggacgt
     1321 ttgcaggcca ttttgcaggc gcatgaaaat gaactgggcg atttcgtgct gcatatggat
     1381 gggctggcga atgattttct ccctgacgct ggccgctggc agtggcgcta ctggggaaaa
     1441 ggcagtttta caccgatgaa tgccacctgg gatgtcgcag gaaaaggtga gtggcatgac
     1501 agcacgatta cgctgaccga tctctccacc ggtttcgacc agttacaata cggtacgatg
     1561 acggtagaaa agccgcgatt aattctcgac aagcccgtcg tctgggtacg tgacgcacag
     1621 catccctcct tcagcggcgc gctgtcactg gacgccgagc aaacgctgtt cactggcggc
     1681 agtgtgttac cgccatcaac cttaaaattt agcgtcgatg ggcgcgatcc gacgtatttc
     1741 ctctttaaag gcgatttaca tgctggtgag attggcccgg ttcgggtaaa tggtcgctgg
     1801 gacggtattc gtctgcgcgg taacgcctgg tggccaaaac aatcactgac tgtattccag
     1861 ccgctggtgc cacccgactg gaagatgaac ttacgcgatg gtgaactgta tgctcaggtt
     1921 gcattttctg ctgcgcctga acaaggattc cgcgcgggag gacacggcgt gttgaaaggc
     1981 ggtagtgcct ggatgccaga taatcaggtt aacggtgtcg attttgtcct gcctttccgt
     2041 tttgccgatg gagcctggca tctggggact cgcggccccg ttacgttgcg aattgccgaa
     2101 gtgattaatc tggtgacagc gaaaaatatt acggctgatt tgcaagggcg ttatccgtgg
     2161 actgaagaag aacccttgct gttgactgat gttagcgtcg atgtgttagg cggtaacgta
     2221 ctgatgaaac aattacgtat gccgcaacat gacccggcgc tgttgcggct gaataatctc
     2281 tcatccagcg aactggttag cgccgtcaat ccgaaacaat tcgccatgtc cggggcattt
     2341 agtggtgcat tgccgttatg gctaaacaac gaaaaatgga tagtgaaaga tggttggctg
     2401 gcaaatagcg ggccaatgac cttgcggctg gataaagaca ccgcagatgc ggtggtgaaa
     2461 gacaatatga ctgcgggttc agcaattaac tggttgcgct atatggaaat tagccggtca
     2521 tcgacaaaaa ttaacttaga taatctcggt ttattaacca tgcaggccaa cattacaggt
     2581 accagtcgcg ttgatggtaa aagcggtacg gtaaacctta attactatca tgaagagaat
     2641 atttttacgc tgtggcgcag tttacgcttt ggcgataatc tccaggcatg gttggagcag
     2701 aacgcacgtc tgccgggaaa tgactgtccg caaggaaaag agtgtgagga aaaacaatga
     2761 aaattttact ggctgcgttg acgtcatctt ttatgctggc tgcctgtacg ccgcgtattg
     2821 aagtcgctgc gcccaaggaa ccgatcacta tcaatatgaa cgttaaaatt gagcatgaga
     2881 tcatcatcaa ggcagacaaa gatgtcgaag agctgcttga aactcgtagc gatcttttct
     2941 gaggcgatga tgaagaaaac attacttctt tgtgcgtttc ttgttgggct ggtaagcagc
     3001 aatgtaatgg cattgactct ggatgaagcc agaactcagg ggcgggtagg tgaaacattt
     3061 tatggttatc tggttgcgct gaaaacggat gctgaaacag agaaattagt aaccgacatt
     3121 aatgccgaac gtaaagcgag ttaccaacaa ctggcaaagc aaaataatgt gtctgtagat
     3181 gatatcgcga aactcgccgg gcaaaagctc gtagctcggg ccaaaccggg ggaatatgta
     3241 caagggataa acggtaaatg ggtgcgaaaa ttttaatagc aaaaccgcaa tatcgggata
     3301 acgctacagg agaaagcgat tgatctattt tcctgaaaca aggtgaatat tcaaaaactc
     3361 ctgtcaaatt gcctttcgcc ctgaaaaatg catagtcaaa tatctgtttt aactaattgg
     3421 cgttgcagta cacacaacgc caattagtta gcggaattta cgtcgatact cgcctggcgt
     3481 catcccaaag cgttgcttaa ataccgttga aaaatgactc tggtcagaaa atccccaatg
     3541 aaagccgatg cctgccagtt tttcatcatc tgcggcatgg cgaatcgcat ctgcacaaaa
     3601 atcgagacga cggttacgaa tatattgcgc gactaccaaa cctttatcgg caaacattcg
     3661 gtacaaacta cgtactgaca tacctgtctc tccggctatc cactccgggc gtaatatctc
     3721 ttcgcgaata ttatcgtcta tcaacgtaac cactttttga aactgacgtt cacgacgagg
     3781 ttgaacagat tcccgctgat gaagtaccgg gcgcagcaga cacaccatcg cctgtagcgc
     3841 agcttcactt tctgtttcag aaagtgccgg attattcatg ctctcctgta acaggcgatg
     3901 actgagttgc accatgggta agtcagcgtc cagtctttct gcgcagacag gtttttgatg
     3961 gggaaaatat tgttccagca gagtgcgtgg caaaagtaat gaaatctgtt tagaagactc
     4021 ctgccagtaa agcgaacagg ggcgtgaggc atcgagtaac gtaatatcgc cagcgccaat
     4081 ctgcacctga cgctcatcct gctccattat tgcctgacca ctaagctgaa aaacggtgta
     4141 aaaccaggca tcgtcgctgc cttttacctc ctgccaggtg cgggataaat tcaccccgct
     4201 ggttgtcacg gtactcagct ttagtccttt ggcaaaatgc gtgtccagta cacccgtgta
     4261 acgctcagtc agcaggcgtc cggtaaaatt tccgcatacc tgattgattt gggaaagcca
     4321 ttgctgaaac tcattatcca ttgcggggtt catggcacgt tttcgctctg tgaaatgtat
     4381 ttttattgtt gcatttgtgt tgcaataaac gaagctaatg agcctgacta taggaaataa
     4441 gtcttgtcag gcatagagac ataagcggtt attgtcacga tttgcggagc ttgtcacagc
     4501 tgacaaagcg aatgtcacag cgaaaaaagt gacttttctt gtcgctgcgt acactgaaat
     4561 cacactgggt aaataataag gaaaagtgat gacagagccg catgtagcag tattaagcca
     4621 ggtccaacag tttctcgatc gtcaacacgg tctttatatt gatggtcgtc ctggccccgc
     4681 acaaagtgaa aaacggttgg cgatctttga tccggccacc gggcaagaaa ttgcgtctac
     4741 tgctgatgcc aacgaagcgg atgtagataa cgcagtcatg tctgcctggc gggcctttgt
     4801 ctcgcgtcgc tgggccgggc gattacccgc agagcgtgaa cgtattctgc tacgttttgc
     4861 cgatctggtg gaacagcaca gtgaggagct ggcgcaactg gaaaccctgg agcaaggcaa
     4921 gtcaattgcc atttcccgtg cttttgaagt gggctgtacg ctgaactgga tgcgttatac
     4981 cgccgggtta acgaccaaaa tcgcgggtaa aacgctggac ttgtcgattc ccttacccca
     5041 gggggcgcgt tatcaggcct ggacgcgtaa agagccggtt ggcgtagtgg cgggaattgt
     5101 gccatggaac tttccgttga tgattggtat gtggaaggtg atgccagcac tggcagcagg
     5161 ctgttcaatc gtgattaagc cttcggaaac cacgccactg acgatgttgc gcgtggcgga
     5221 actggccagc gaggctggta tccctgatgg cgtttttaat gtcgtcaccg ggtcaggtgc
     5281 tgtatgcggc gcggccctga cgtcacatcc tcatgttgcg aaaatcagtt ttaccggttc
     5341 aaccgcgacg ggaaaaggta ttgccagaac tgctgctgat cacttaacgc gtgtaacgct
     5401 ggaactgggc ggtaaaaacc cggcaattgt attaaaagat gctgatccgc aatgggttat
     5461 tgaaggcttg atgaccggaa gcttcctgaa tcaagggcaa gtatgcgccg ccagttcgcg
     5521 aatttatatt gaagcgccgt tgtttgacac gctggttagt ggatttgagc aggcggtaaa
     5581 atcgttgcaa gtgggaccgg ggatgtcacc tgttgcacag attaaccctt tggtttctcg
     5641 tgcgcactgc gacaaagtgt gttcattcct cgacgatgcg caggcacagc aagcagagct
     5701 gattcgcggg tcgaatggac cagccggaga ggggtattat gttgcgccaa cgctggtggt
     5761 aaatcccgat gctaaattgc gcttaactcg tgaagaggtg tttggtccgg tggtaaacct
     5821 ggtgcgagta gcggatggag aagaggcgtt acaactggca aacgacacgg aatatggctt
     5881 aactgccagt gtctggacgc aaaatctctc ccaggctctg gaatatagcg atcgcttaca
     5941 ggcagggacg gtgtgggtaa acagccatac cttaattgac gctaacttac cgtttggtgg
     6001 gatgaagcag tcaggaacgg gccgtgattt tggccccgac tggctggacg gttggtgtga
     6061 aactaagtcg gtgtgtgtac ggtattaatc tggttcgctc ataagtaaaa aacggcacct
     6121 ggtgccgttt ttttgtctga aacaatcact tatctttctt cagcgcccct agcgttggcg
     6181 tttcgtcaaa gaagttccat ggtttaagca gagtatgtac ccattcggtc ggcataatcg
     6241 gccactcttc ggcgcgggcc acatgtgtgg tgccggtggt catccagaca acggcgtcgg
     6301 tgttgtccag cgactcgtta tctttactgt attgtccaag accggtgtca tgagtagaac
     6361 ggttcggata tttgccttcc gggaaacgct cgccaggatg ataacgcgtt acccaaagct
     6421 gcttgtccat aaagcttaaa cgatgataga tccactcgtc cggcgcgaac tgggcacctt
     6481 ttgctaccgg gtgagtacca cctgcataag gaataatttg ataggaaact ggattgccca
     6541 tgcggttctc tttgttcggg ttactcaaca gacgaatcgt gcccggatca aacttctgcg
     6601 cggcatcctg ttcattgccg atgttgtact gattaacttg catggtactg gtgcgtgggc
     6661 caccggcagt attcggtttt accactgggt ccatcgccac caggctgtta ttctcgccat
     6721 ctacatccag atcgaggcgg aaattataaa tatgttggtg tgtagtaccc acgatattgt
     6781 gatcgataag cgtgccgtag cgcgtgtcat ctttcgccgt ctcatcgtgc atggttttcg
     6841 ctttaacacc tttcaccgct tcgatgcccg tagcaccggc atcaatgcca atagtgccgt
     6901 tttcatggaa gatccagtca aaaatgtagt catagttacc cactgtactg atccagcgca
     6961 ccactaactc ccggcgttcg gtactgacgt tgggctggcc catttcctga tgcttatact
     7021 ccggcccagc ataacgttca aataccgcga tagcgcgagg gatctccatc ggcacgccag
     7081 tgtagtcggc gatggtttca ttaaggagca ctgcgttaga cggggcatct ttaccacgag
     7141 caattggtga ggttagcgtg cccataccgt agtcaccaga gtccagatac gctttaaagt
     7201 accagccaat atcaggatca ccgtaaggca caatcatgcc gccgagagaa ccttcgtaca
     7261 tgactttgcg tttggtgcca ttgtcgttat aagtcacggt ggagatcatc ggcccgacgc
     7321 gagagttcat gctgaggtga aaatcccagt tccgccagtg aatcatatcg ccagtaatgg
     7381 tgtaattttt accttcaggc tcaatgattt gcataggctt aactgccgga gcaacgcggt
     7441 cacggctatc aaatgggcgt gcggtcattg gcaccggaac taccggacct tcttcaatct
     7501 taacgatttt tttctgttct aaatcaacga ccgccaccag gttttcgatg ggatgtgccc
     7561 agtagttgcc atcaccgaca tcaagatagc tgatgacttt gagcaaccgg gcatcttgtt
     7621 tcaggccatc tttaccatcg aaataaccta cggtcagcgg cgtggtaatc acttttttcg
     7681 catcagtaat accgcgtttc ttcacggcag cggcaaattc ttcactgttg ttaataatgt
     7741 tctgcacact ggcgaaatca tccagcaaca ccataccgtg ggcgtcttta atgggttgcc
     7801 aggagagcag tttgttgttt tgcagatcca ccaccgcttc gatgatatgt ttgccgtcga
     7861 gcataatgac gtcggctttg cgcggctggt caaccggttt gttttccagc gcaaacgccc
     7921 agacagcttc tttatctggc ggtagcaggg agatctcagt aaaacgggta ttgggtttga
     7981 agtccgcgga agctttaaca atttcaacgg cctgtttaat ttcgtccgca gttagcgcat
     8041 taagtgggtg agggcgcttt tctacctgaa aggtttgatc cagaccggac tggaaaacat
     8101 cgttaataaa ggtgtcagaa acccaggcct tattgtcttt catcactacc ggcacttgca
     8161 gtgccagagg ttgaccatta acaattgctg tttgcgcacc aggcttcact ttcacgtacg
     8221 cgccatcttt aatcagggta aagatctggg cgtagtcgtc ccactgcaca tcggcaccaa
     8281 attctttaag cgtttcatcc attggcacca tatgcgcttc accaccgtgg gcaaataccg
     8341 gcgcttgcca ggcgaaactt aaggcgactg ccaacgccag ggttgtttta cgggcagaat
     8401 acagagaggg gcttcccatt attaacctcg tcagatgttg tgttcttgtt agcaaccgcg
     8461 ctctgtgggc ggtttagtca ggttcacatt atcagtactg atgcaaaggg gattgcctgc
     8521 acctgccagg ttgtttggca ggtgtgccag cttttcatac agtgggtgcc ctgaaaatag
     8581 atgtacacat catgcataat gtgacaacgt cacaaaactt agtgaaataa aagggcaact
     8641 attggccgtt gcccttcatt caccgattaa tcgacaaaat caccgtgctg cctggccacc
     8701 agcgtcagaa ttgaatacag cgccaccggg gtttgatgct gattgaatac ctctaccgcc
     8761 cattccacca cacctgttgg tttttcttct gcgctacgct gttttttcag cgtcttgcgc
     8821 ttacaggtga gacgcacctg gatggtatcg cctggcttta cgggttcgat aaagcgcaag
     8881 ttttccagcc cataattagc aatgaccgga ccgacaccgg catcgacaaa cagacccgca
     8941 gccgcagaaa gcacaaaata cccatgcacc acacgctcac cgaaaataga ttcggcagca
     9001 gcaatcttat ccatatgcgc atagaaatga tcgccgctga ggcaagcaaa gttaacaata
     9061 tcggcctctg tcattgtgcg gcggggagtc agcaggctgt cgcctggttg tagctcctca
     9121 aaatatttgc ggaacggatg aatacgatct tcttcgactt tcgcaccacg cacccactgt
     9181 ttactgatag ctgcaagcat cgtcggacta ccctgaacag cggttcgctg catgtaatgt
     9241 ttcaccgctc gtaaaccacc taattcttca ccgcctcctg cgcgaccagg cccaccatgt
     9301 aacagttgtg gcagtgggga gccatgcccg gtggattctt ttgccgattc ctcattgagg
     9361 atctgaattc gcccatgcgt acgtgccgcg tcggtaataa actgacgcgc aatttgcgga
     9421 tcagccgtca ccagcgttcc cgcaaggcta ccgccgcctg cacaagccag ttgcagagca
     9481 tgtcgctggt tttgtgctgg catcagcgtt gcgacagggc caaaggcttc tgttgcatgt
     9541 accgccggtg tttcatccgg ctgcggacag tacaataagg ttggcgggaa gaaggcaccc
     9601 gcagcagata aatccgcctg accaccgagg cgaatctcgc atcctgcagc cagcaatatg
     9661 ttcacttttt cctgcacatc ggcacgctgc tcagcattta ccagtgcgcc cattttcacg
     9721 ccttcctgag caggatcacc gaccacgact ttctgtaatc gcgcaaccag agcatcactg
     9781 acagcattaa ccaatgcctg cggcacaata atccgccgga ttgccgtaca tttttgcccg
     9841 gcttttgtgg tcatctcacg cacaacttca cgaataaaca gcgcaaactc cggttgatcc
     9901 ggggtgacat cttcgcccag tacgcagcag ttcagggaat cagcttccat agtgaagggg
     9961 atagatttgg cgacgatatt tggctgaact cgcagcatct gtccggtcgc cgctgacccc
    10021 gtgaaagtca ccacatcctg gctgtccaga tgatccaaaa ggtcgcccgc actaccgcag
    10081 atcagactaa ttgcgccttc gggaacaaga ccactatcga caattgattt caccatcgcc
    10141 tgagtcagtt gggccgtcgc ggtagctggt ttgatgatgg ctggcattcc gcccagccac
    10201 gttggtgcca gcttttccag cattccccag caggggaagt taaaggcgtt aatatgcact
    10261 gccacgcctg actttgaggt cagtacatgg cgcgcggcaa atccaccttc tttcgataag
    10321 gggatcaatt catcttccgg ccacagcgta tcgtcaggca gctcccggct accgaggctg
    10381 gcgtaagtaa ataacgtccc aatgccacct tcaatatcaa cccaactgtc tgcccgcgtt
    10441 gcgcctgttt gcgcagaaag agcatagaag cgctcttttt cactcagcag atgtttagcg
    10501 accgctttaa gcatcgccgc acgttcgata aaggtcatcg cgcggagggc gggggcacct
    10561 ttttcaatgg caaactggcg ggcagccgcc atatcaagac cttcactggt cacttcccat
    10621 aacgcctcgc cgctaatagc gtggtgaatc aaacggctac ggccccggcc agactgccag
    10681 gtaccggata agaaactggc taactgctgc atcgctactc tccagatgtt tcacatttat
    10741 gttgctaata gttaaatcgc gaatcataaa aagcaagggt tcttttaacg aaatgttaac
    10801 tatgcgatct gtatagcaac tgcggaaaac attaatgcac tgataaataa tgatttataa
    10861 aaatagggtg cgaaatccgt cacagttcaa gcatacaaaa tttgtgattt tagttaacca
    10921 ttgtgtaact ttcataaaac aatgtgattc gtgtttttaa ttaattcacg aaaactggaa
    10981 tcgtaaaggt gatgacgtga cccaagaaga acgctttgag caacggatag cccaggaaac
    11041 ggctatcgag ccacaggact ggatgcccga tgcttaccga aagacactga tccgccagat
    11101 tgggcagcat gcgcactccg agattgttgg tatgttgcct gaaggtaact ggatcactcg
    11161 cgcaccaacc ttgcggcgta aagccattct gttggccaaa gtgcaggatg aagccggtca
    11221 tggtctgtat ctctatagcg ccgccgaaac actgggctgc gccagagaag acatctacca
    11281 aaaaatgctc gacggacgga tgaaatattc ctccatcttt aattatccga cattgagttg
    11341 ggctgatatc ggcgttatcg gctggctggt ggatggcgca gcgatcgtca atcaggtggc
    11401 gttatgtcgc acttcttatg gcccgtatgc cagagcaatg gtgaaaatct gcaaagaaga
    11461 gagttttcac cagcgtcagg ggtttgaagc ctgcatggca ctggcgcagg gcagcgaagc
    11521 tcaaaagcag atgttgcaag acgccattaa ccgtttctgg tggccagcct taatgatgtt
    11581 cgggccaaac gatgataact cgccaaacag cgccagaagt ctcgcctgga aaatcaaacg
    11641 tttcaccaac gacgaactcc gccagcgttt cgtggataac accattccac aggttgaaat
    11701 gctcggtatg accgttcctg acccggatct gcattttgac actgaaagcg gtcactaccg
    11761 ctttggtgag atcgactggc aggagtttaa cgaagtaatt aacggtcgcg gaatttgtaa
    11821 tcaggagagg ctcgacgcca aacgtaaagc ctgggaagaa ggtacctggg tacgggaagc
    11881 agcgctggcc catgcacaaa aacaacttgc ccgtaaggtc gcataaggag attcaaaatg
    11941 agtaatgttt actggccgtt atacgaagtt ttcgtgcgtg gcaaacaggg cttatcacac
    12001 cgccatgttg gcagtttaca tgctgccgat gagcggatgg cactggaaaa tgcccgtgat
    12061 gcttacaccc gtcgtagcga aggatgttca atttgggtgg tgaaggcgag tgaaattgtt
    12121 gcctcgcaac cggaagaacg cggtgaattt tttgatccgg ctgaaagcaa ggtctatcgc
    12181 catccaacgt tttacaccat ccctgatggc attgagcaca tgtgaggtcg gaaatgaatc
    12241 agttaacggc ttacaccttg cgcctgggcg ataactgcct ggtgctctcc cagcggttgg
    12301 gtgaatggtg cggtcatgca ccggaactgg aaatcgatct cgcactggca aatattggac
    12361 tcgatttatt aggccaggca cgcaacttct tatcgtatgc cgctgaatta gcgggagaag
    12421 gcgatgaaga taccctggcc ttcacccgag acgagcgcca gttcagcaac ttattgttgg
    12481 ttgaacagcc aaacggcaat tttgccgaca ccattgcacg ccagtatttc atcgatgcat
    12541 ggcatgtggc gctctttacc cgtctgatgg agagccgtga tccgcaactg gcggcgattt
    12601 ctgccaaagc aattaaagaa gcgcgttatc acctgcgttt tagtcgtggc tggctggagc
    12661 gactgggcaa tggtactgac gtatcagggc aaaagatgca gcaggccatc aacaagttgt
    12721 ggcgttttac cgccgaactg ttcgatgccg acgagattga tattgcactg agtgaagagg
    12781 gtattgcggt tgatccacgc actttacgcg cagcgtggga agccgaagtt tttgccggga
    12841 tcaacgaagc cacattgaac gtaccgcaag agcaggcgta tcgcactggc ggtaaaaaag
    12901 gactgcatac cgaacacctt ggacccatgc tggcagaaat gcagtatctc cagcgtgtct
    12961 tgcccggtca gcaatggtaa cagaggagat gggtatgcaa cgtctggcca ccattgcacc
    13021 gccacaggtt catgagatat gggcgctact cagccagatc ccggacccgg agatcccggt
    13081 gctgaccatt accgatttag gcatggtgcg taatgtgaca cagatgggag aaggatgggt
    13141 gatcggcttt acgccgactt attccggttg tccggcaacg gaacatttga ttggcgcgat
    13201 acgtgaggca atgacaaccc acgggtttac ccccgttcag gttgtgctgc aactcgaccc
    13261 ggcatggacc accgactgga tgacccccga tgcccgtgag cgtctgcgac agtatggcat
    13321 tagcccgccc gccggacaca gttgccatgc ccatttgccg ccagaagtac gttgcccgcg
    13381 ctgcgccagc gtccatacca cacttatcag tgaatttggt tccacggcct gcaaagcatt
    13441 gtaccgctgc gatagttgcc gcgaaccttt cgattatttc aaatgtattt gaggatgcca
    13501 tgacaacgtt tcattcctta acagtggcaa aagtcgagcc ggaaacccgt gatgcggtga
    13561 ccattacctt tgcggtgccc cagcctttgc aggaggcgta tcgctttcgc cccggtcaac
    13621 atttgacctt aaaagccagt cttgatggtg aagaattacg ccgttgttac tccatttgcc
    13681 gcagctacct gcctggcgaa attagtgtgg cggtgaaagc cattgaaggc ggacgtttct
    13741 cccgctatgc ccgcgaacac atccgccagg gtatgacgct ggaggtcatg gtgccgcagg
    13801 ggcatttcgg ctatcagcca caggccgaac gccagggccg ctatctggct attgcagcag
    13861 gatcaggtat tacgccaatg ctggcgatta tcgccaccac tttacaaacc gagcctgaaa
    13921 gtcagttcac cctgatctac ggtaaccgta ccagccagag catgatgttt cgccaggcac
    13981 tggcagacct gaaagacaaa tatcctcagc gtttacagtt gttgtgcatt ttcagtcagg
    14041 aaaccctcga cagcgatctg cttcacgggc gtattgacgg tgaaaaatta cagtcacttg
    14101 gggcctcgct cattaatttt cgtctttatg atgaggcatt tatttgtggt ccggcggcga
    14161 tgatggatga gacggaagcc gccttaaaag cactgggaat gccagataaa accattcatc
    14221 tggagcggtt taatacgcct ggcacgcgcg tcaaacgtag cgttaacgtg caaagtgacg
    14281 gacaaaaagt gactgtacgt caggatgggc gggatcggga aatcgtgctt aatgccgacg
    14341 atgaaagcat tctcgatgcg gcattgcgcc agggggctga tctgccctat gcctgcaaag
    14401 gcggcgtctg tgcgacctgc aaatgcaaag tgctgcgtgg caaagtggcg atggaaacca
    14461 attacagtct ggaaccggat gaactggccg caggttatgt gttgagttgc caggcactgc
    14521 cgctgaccag cgatgtggtg gttgactttg acgcgaaggg gatggcatga gcgaactgat
    14581 cgtcagccgt cagcaacaag tattgttgct gacccttaac cgtcccgccg cacgtaatgc
    14641 gctaaataat gcccttctga cgcaactggt aaatgaactg gaagctgcgg ctaccgatag
    14701 cagcatttcg gtctgtgtga ttaccggtaa tgcacgcttt tttgccgctg gggccgatct
    14761 caacgaaatg gcagaaaaag atctcgcggc caccttaaac gatacacgtc cgcagctatg
    14821 ggcgcgattg caggccttta ataaaccact tatcgcagcc gtcaatggtt acgcgcttgg
    14881 ggcgggttgc gaactggcat tgttgtgcga tgtggtggtt gccggagaga acgcgcgttt
    14941 tgggttgccg gaaatcactc tcggcatcat gccaggcgca ggaggaacgc aacgtttaat
    15001 ccgtagtgtc ggtaaatcgt tagccagcaa aatggtgctg agcggagaaa gtatcaccgc
    15061 tcagcaagca cagcaggccg ggctggttag cgacgtcttc cccagcgatt taaccctcga
    15121 atacgcctta cagctggcat cgaaaatggc acgtcactcg ccgctggcct tacaagcggc
    15181 aaagcaagcg ctgcgccagt cgcaggaagt ggctttgcaa gccggacttg cccaggagcg
    15241 acagttattc accttgctgg cggcaacaga agatcgtcat gaaggcatct ccgctttctt
    15301 acaaaaacgc acgcccgact ttaaaggacg ctaatgatgg aattcatcct cagtcatgta
    15361 gaaaagggcg tgatgacact aacgctcaac cgcccggaac gcctgaacag ttttaatgat
    15421 gagatgcacg cacaactggc agagtgcctg aaacaggtcg agcgcgacga cactatccgt
    15481 tgcctgttac ttacaggtgc cgggcgcgga ttttgtgctg gtcaggatct taacgatcgt
    15541 aacgttgatc ccacaggccc cgcaccggat ttagggatgt cagttgaacg tttctataac
    15601 ccactggtac gacgcctggc aaaactgcca aaaccggtga tctgtgcagt caatggcgtg
    15661 gcggcagggg caggcgcaac actggcactg gggtgcgaca tcgttattgc tgcccgttca
    15721 gcaaaattcg tcatggcgtt tagtaagtta ggcttaatac ccgattgcgg tggaacctgg
    15781 ttactgccac gcgttgctgg gcgagcgcgc gccatggggc tggcactgct ggggaatcaa
    15841 ctgagtgctg aacaggcgca cgaatggggg atgatctggc aggttgttga tgatgaaacg
    15901 ctggcagata ccgcgcaaca gctggcacgg catctggcga cacaaccgac atttggtctt
    15961 ggacttatca agcaagcgat aaatagcgct gaaaccaata cgctcgatac gcaactggat
    16021 ctggaacgtg actatcagcg acttgccgga cggagcgcag attatcgtga aggtgtcagt
    16081 gcgttcctgg ctaaacgctc accgcagttc acggggaaat agcatgatga taaatgtgca
    16141 aactgtggca gtgattggga gcggcaccat gggggcaggc attgctgaag ttgccgccag
    16201 tcatggacat caggttttac tgtatgacat ttctgctgaa gcgctgaccc gcgcaatcga
    16261 cgggatacac gcgcggctaa attcacgcgt gacgcgggga aaactgaccg ctgaaacctg
    16321 tgaacgcaca ttgaaacgcc tgatcccggt gaccgatatt cacgcgctgg cagcagcgaa
    16381 tctggttatt gaagcggcgt ctgaacgtct ggaagttaaa aaagcgctct ttgcacaact
    16441 ggcggaagtt tgcccgccac aaacgctatt gaccactaac acttcgtcaa tctctataac
    16501 cgcgattgct gcggaggtaa aaaatcctga acgtgttgcg gggctgcatt tttttaaccc
    16561 ggcaccggtg atgaagttgg tggaggtggt cagtgggctg gcaacggcag cggaagttgt
    16621 cgagcagttg tgtgaactga cattgagttg gggtaagcag ccagtgcgct gtcattcgac
    16681 ccccggattt atcgttaacc gtgttgcgcg tccttattat tccgaggcct ggcgggcact
    16741 ggaagagcag gttgctgcac cagaagtgat tgacgctgca cttcgcgatg gcgctggttt
    16801 cccgatgggg ccgctggaat taaccgatct gattggtcag gacgtcaatt ttgctgtcac
    16861 ctgttcggtg tttaacgctt tctggcagga gcgtcgtttt ttaccttcgc tggtgcaaca
    16921 ggaactggtg attggtggac ggttgggcaa gaaaagtggg ctgggcgtgt acgactggcg
    16981 cgcggaacgt gaggcagttg ttggcctgga agcggtaagc gacagtttta gcccaatgaa
    17041 agtagaaaag aaaagtgacg gtgtcacgga aattgacgat gttttattga ttgagacaca
    17101 aggcgagacg gcacaggcgc tggcaacacg actggcgcgc ccggtggtag ttatcgacaa
    17161 aatggcgggc aaggtggtga ccattgctgc ggcagcggtg aacccggact cagcgaccca
    17221 caaggccatt tattacctgc aacagcaggg caaaacagtg ctgcaaattg cagattaccc
    17281 aggaatgctg atttggcgaa cggtagcaat gatcatcaat gaagcccttg atgcgctgca
    17341 aaaaggcgtg gcctctgaac aggatatcga taccgccatg cgtcttgggg tgaattatcc
    17401 atatggcccg cttgcctggg gagcgcaact tggctggcag cgaatattaa ggctcctgga
    17461 aaatctacaa catcactatg gcgaagaacg ctatcgccca tgttcattgc tgcgccaacg
    17521 ggcgcttctg gagagcggtt atgagtcata aggcctggca aaatgcccat gcaatgtatg
    17581 agaacgatgc ctgcgcgaaa gcgcttggca tcgacattat ctcaatggat gaaggctttg
    17641 ctgtagtgac catgaccgtc actgtacaaa tgcttaatgg tcatcaaagt tgccacggcg
    17701 ggcagctatt ttcactggct gatactgcct ttgcctacgc ctgcaatagc caggggttgg
    17761 cagctgtcgc ttctgcctgc acgattgatt ttttacgtcc aggctttgcc ggagacacct
    17821 taactgcgac tgcgcaggtt cgtcatcagg gcaagcaaac cggtgtttac gacatcgaaa
    17881 ttgttaacca acaacaaaaa acggttgcgc tgtttcgcgg taaatctcac cgcatcggcg
    17941 gcaccattat aggagaagcc tgatgcgtga agcctttatt tgtgacggaa ttcgtacgcc
    18001 aattggtcgc tacggcgggg cattatcagg tgttcgggct gatgatctgg ctgctatccc
    18061 tttgcgggaa ctgctggtgc gaaacccgcg tctcgatgcg gagtgtatcg atgatgtgat
    18121 cctcggctgt gctaatcagg cgggagaaga taaccgtaac gtagcccgga tggcgacttt
    18181 actggcgggg ctgccgcaga gtgtttccgg cacaaccatt aaccgcttgt gtggttccgg
    18241 gctggacgca ctggggttcg ccgcacgggc gattaaagcg ggcgatggcg atttgctgat
    18301 cgctggtggc gtggagtcaa tgtctcgggc accgtttgtt atgggcaagg caaccagtgc
    18361 attttctcgt caggctgaga tgtttgatac cactattggc tggcgatttg tgaacccgct
    18421 catggctcaa caatttggaa ctgacagcat gccggaaacg gcagagaatg tagctgaact
    18481 gttaaaaatc tcgcgagaag atcaagatag ttttgcgcta cgcagtcagc aacgtacggc
    18541 aaaagcgcaa tcctcaggca ttctggctga ggagattgtt ccggttgtgt tgaaaaacaa
    18601 gaaaggcgtt gtaacagaaa tacaacatga tgagcatctg cgcccggaaa cgacgctgga
    18661 acagttacgt gggttaaaag caccgtttcg tgccaatggg gtgattaccg caggcaatgc
    18721 ctccggggtg aatgacggag ccgctgcgtt gattattgcc agtgaacaga tggcagcagc
    18781 gcaaggactg acgccgcggg cgcgtatcgt agccatggca accgccgggg tggaaccgcg
    18841 tctgatgggg cttggtccgg tgcctgcaac tcgccgggtg ctggaacgcg cggggctgag
    18901 tattcacgat atggacgtga ttgaattgaa cgaagcgttc gcggcccagg cgttgggtgt
    18961 actacgcgaa ttggggctgc ctgatgatgc cccacatgtt aaccccaacg gaggcgctat
    19021 cgccttaggc catccgttgg gaatgagtgg tgcccgcctg gcactggctg ccagccatga
    19081 gctgcatcgg cgtaacggtc gttacgcatt atgcaccatg tgcatcggtg tcggtcaggg
    19141 catcgccatg attctggagc gtgtttgagc atatcaacct gcgagtaccc tacaatgata
    19201 accaatacaa agcttgaccc gattgaaacc gcgtctgttg atgagttaca ggcgttgcaa
    19261 acacagcgtc tgaaatggac gctcaaacac gcgtatgaaa atgtcccgat gtatcggcgc
    19321 aaattcgacg cagcaggcgt acatcctgat gatttcaggg aactttcaga cctgcgtaaa
    19381 tttccctgta ccaccaaaca ggatctgcgc gacaactatc cctttgacac ctttgccgtg
    19441 ccgatggaac aagtggtgcg cattcatgct tcttcgggaa ccacaggtaa accgacagtt
    19501 gtcggttata cgcaaaacga tattgataac tgggccaata ttgtagcgcg ttctttgcgt
    19561 gcggcagggg gctcgcccaa agacaaaatt catgttgcct atggttacgg cctgtttact
    19621 ggtgggctgg gcgcgcacta tggtgccgaa cgtctgggcg ctacggtgat cccgatgtct
    19681 ggcgggcaga cggaaaaaca agcgcaactg atccgcgatt ttcaaccaga tatgatcatg
    19741 gttacgccat cttattgcct taacctgatt gaagagctgg agcggcagtt gggcggtgat
    19801 gccagcggtt gctcgctgcg ggttggcgtg tttggtgctg agccgtggac acaggccatg
    19861 cgtaaagaga ttgaacggcg tctggggatc accgcactgg atatttatgg cctgtcagaa
    19921 gtgatggggc cgggggtggc gatggagtgt ctggaaacta ccgacggtcc aactatttgg
    19981 gaagatcatt tctatcctga aattgttaat cctcatgacg gcacaccgct tgccgatggt
    20041 gaacatggcg aactgttatt caccacgctg accaaagaag cattgccggt cattcgttac
    20101 cgcacgcgtg atttaacccg actgttacca ggaacggcgc ggactatgcg ccgcatggat
    20161 cgcatcagcg gacgcagcga tgacatgttg attattcgcg gtgtaaatgt ctttccgtca
    20221 caactggaag aagagattgt caaattcgaa catttatcgc cgcattacca actggaggtg
    20281 aaccgccggg ggcatcttga ttcactttct gtgaaagtgg agttgaaaga aagtagcctg
    20341 acattgacgc atgagcagcg ttgccaggta tgccaccagc tgcgccatcg gattaagtcg
    20401 atggtgggga tctctaccga tgtgatgatc gttaactgtg gcagtatccc gcgttcagaa
    20461 ggcaaggcgt gtcgggtgtt tgatctgcgc aatattgttg gtgcctgacg tgtcgtctgg
    20521 ccctggtgag gtaaagcgtc agggccagaa gtcgatacga cctgtgctat gattcataaa
    20581 tcacaacaat aacaacagac tgaatcgaat gagtaaactt gatactttta tccaacatgc
    20641 tgtaaacgct gttccggtca gtggcacatc tttgatctcc tctctgtatg gtgattcgct
    20701 ttcccatcgt ggtggtgaaa tctggcttgg tagtctggct gctttgctgg aagggctggg
    20761 atttggtgag cgtttcgtgc gcactgcttt gtttcgtctt aataaagaag gctggctgga
    20821 tgtttcccgc atcgggcgac gcagtttcta tagcctcagt gataaaggct tgcgcctgac
    20881 gcgacgggca gaaagtaaaa tttatcgcgc agagcaacct gcatgggatg gtaaatggct
    20941 cctgttgctc tcggaaggtt tagataaagc gacgctggct gatgtcaaaa agcagttgat
    21001 ctggcaaggt tttggcgcac tggcacccag cctgatggca tcgccgtcgc aaaaactggc
    21061 ggatgtacag acacttttgc atgaagcggg ggtggcggat aacgtgattt gttttgaagc
    21121 gcaaatacca ctggcgcttt ctcgcgcagc actgcgtgcc agagtagaag agtgctggca
    21181 tttaactgaa caaaatgcca tgtacgaaac ctttattcag tcattccgcc cgctggtgcc
    21241 gctgttaaaa gagacggcag acgagttaac cccggagcgt gcatttcata ttcagctttt
    21301 actgatccat ttttatcgcc gtgtcgtcct taaagaccca ttgttgccgg aggagttgct
    21361 tccggctcac tgggcagggc atacggcgcg tcagctgtgt atcaacattt atcagcgcgt
    21421 agcgcctgct gctttagcgt tcgttagtga aaaaggtgaa acctcagtcg gtgaactgcc
    21481 tgcgccggga agcctgtatt ttcaacgttt tggcggcttg aatattgaac aggaggcgtt
    21541 atgccaattt accagataga tggtctgact ccggttgtgc cagaagagag ctttgtccat
    21601 ccgacagcgg tattgatcgg cgatgttatt ctcggcaagg gcgtttacgt tgggcctaat
    21661 gccagcctgc gtggcgattt tggtcgtatc gtggtgaaag atggcgcgaa cattcaggat
    21721 aattgcgtta tgcacggttt tcccgagcag gatactgtcg tggaagaaga tgggcatatt
    21781 ggtcatagcg ctatccttca cggctgcatt atccgccgca atgcattagt gggaatgaac
    21841 gcggtagtga tggacggtgc ggtgattggc gagaacagca ttgttggcgc atccgcattt
    21901 gtgaaagcca aagcagaaat gcctgctaat tacctgattg tcggcagccc ggccaaagcg
    21961 attcgtgaac ttagtgagca ggagttggca tggaaaaagc agggtacgca tgagtaccag
    22021 gtgctggtga cacgctgtaa gctgacgttg catcaagtcg aaccattgcg ggaagttgaa
    22081 cctggcaggc agcgcctggt atttgatgag aatctgcgac cgaaacagta acgtccagat
    22141 actgaacatt ctaattttta gcatgtgata cctaaaccgc aacgctgata caggttgcgg
    22201 ttttttattg ccggatgcgg tacttgtcgc gttttgtttt gtgtttttca ggacaataga
    22261 gcaactcatc cagtaatctt gtttacgccc tcgcattatt tatctctttt cgtttctata
    22321 ctgatttttc ttaatccgtt ttattacagg gcagggtgcg atgagcagca atacatttac
    22381 tctcggtaca aaatccgtta accgtcttgg ttatggcgcg atgcaactgg caggtcctgg
    22441 ggtttttggc cccccaagag atcgccacgt cgctataacc gtgctgcgcg aggcgctggc
    22501 attgggcgtc aatcacattg ataccagcga cttttatggt ccgcacgtca ccaatcagat
    22561 tatccgcgaa gcgctttatc cttactctga cgacctgaca attgtcacta aaattggtgc
    22621 gcggcgtgga gaggacgctt cctggttgcc cgccttttct ccggcagagt tgcaaaaagc
    22681 ggtgcacgat aatctacgta atctcggcct ggacgtactg gatgtggtta acctgcgcgt
    22741 tattatgggg gatggtcatg gcccagcgga aggatcgatt gaggccagcc tgaccgtgct
    22801 ggcagagatg caacaacaag gcttggtaaa acatattggc ctgagcaacg tcacaccgac
    22861 gcaggttgca gaggcgcgca agattgccga aattgtctgt gtgcaaaacg aatacaacat
    22921 cgcgcaccgt gctgatgatg caatgattga tgctttggcc cacgatggca ttgcctacgt
    22981 gccgttcttc ccgctcgggg gctttacacc gctgcaatcc tctacgctgt cggatgttgc
    23041 tgcgagcctg ggtgcaacac ccatgcaggt ggcgctggcg tggctgttac aacgttcacc
    23101 gaatattttg ctgatcccag ggacgtcttc tgttgcgcat ttacgggaga atatggctgc
    23161 tgaaaaattg catctttctg aggaagtgtt gtctacgttg gatggtattt cgcgagaata
    23221 acgaatatac aaaagggaaa gatgcatttc ccttttgttt cttttttaat ggcatggagt
    23281 gcatatgttt aaggccagga tttgcggctg gataggttta cttcccctgt ttatgctgag
    23341 tcttccagta caggcggaac ttcgatgtgt tgcaaataca gttgatattg aaccattttt
    23401 ttctgcggct accgctgaag ataaacaaca agttgaacaa gctatcaaca gtagcgtgaa
    23461 tcttgtcccc ttcggtttat ctgcatcgga ctggaaagtg catcgtggcg atttagtggt
    23521 agaaggtaat atagagagta atcaaaaatt gattgttctt ggtaatttga cagtcaaagg
    23581 taatatttcc actttctctc tttctaatcc atgggttatt ctcggtaacg tgacggcaac
    23641 gaatatagtt acggattcac cactgttaat taccggttcg ataaatgcaa gtggactggt
    23701 atttatcgac tcatattacg ataatccgtc tacgattaag gggagtatta atgcgcgtgg
    23761 gatatttatc aatgacataa ttgcacctgt tgttgcgtct tcgacaaata gtgaattcat
    23821 ggtccgtgcg agtgacaaaa atgacactga aaatgtcaaa aaagcgctga tgataataaa
    23881 tcctgatgca tattattggg gtctaataaa tgatgaagat gctctgaaag aaatttttaa
    23941 gcgaagcaat attcgcatgg cagggaatgt ctgtaatcag atgaaaaaag aagcgctgtt
    24001 tcgccctaag ccttctcctg agttagtgca ggaattgcaa atgctggatg aaggcaacgt
    24061 tgctgcattt gaaggacgag atattgcgac atttgatctt gccataatgc gaactctccc
    24121 aaggcttaaa ggaatttcag ctaacctacg caaacaactt attaatagca atgatgaaca
    24181 aacgattgaa agtatggcga gatatatgcc tgacaatgag attctggagt tgaccgatca
    24241 gcaattgggt taccagcctg ttgttctggg gttgctcgac cgtgaaccgc tctctgtcga
    24301 aataatgacg cgaatgagtc gtctacctga tggtgtcggt ccgttgaatc ttgcgctacg
    24361 tgaaaatctc cctctggata tcgtaatgac tctggcgaaa agagattggg atatgatcat
    24421 tcaggagctt tataaagatg cgtggttatt gcctgaatcc attattgata gctatatccg
    24481 cagtgatgat tcctctattc gtcaggtcgg tgctggagga caactcacct acaatcaggc
    24541 aatgcagttg gcgaacgatt catcaaacaa tgttgtcaca agcttagcgt tcaagctggc
    24601 agagatgaaa caccatggtc aattattgcg gatgacgcca caagagagtg acaaagttgc
    24661 agcctattta taccaaaaat tcgagaatga cgatgatcta atacgtgtgt tatttttagc
    24721 attgccagat aacttacagt ttaattttgt taaaaggatg gagaaaaaat cccctgccta
    24781 cttttgctgt cgggatatgc aggtaattca ctctgacgct gctctacaac ggttattgac
    24841 acgtttcaac gatcctgagg ggtggagtaa tctggcgaaa aatcagtatc tgagcacgtc
    24901 gatgaaacaa aaaatttggc aacgtgcttt atcgcatcgg aaaaataatc cgaaagcaga
    24961 ttcagctgcg tatgaaacca gcgctgatat gattttgtct gagcttatca gccacggtga
    25021 agttgatgat cagatgctat taaatgccac cgccctgata cgtttggaag actgggactt
    25081 tttagagagt gcattagtta gttgggataa tttacccgct gttgtcctca aggaattaca
    25141 gcaaaatacg ccacgcaatg atatttgggc gaagtttttt ctgaggcagg aaaacagctc
    25201 ccgtgcgcag gttgatgaag cattacgcgt ctattatgca ctcgaccccg atgcgttagc
    25261 acaactggat gtactggcaa aacaaccgga tcgtatatgg tggagtacac tggcgaaaag
    25321 caatctcaca tttttcaagt tcggcgcact taacaaccgc cacacaccgc ctgcagtact
    25381 ggcggcagaa attgatcccg agtggtggat tgtggcgatg aataatcccc gttttccagt
    25441 tgatgtatta aaggcgaggc tgaaacgtga tcctttgctg gcgttagagc ttgttaaccc
    25501 tgaactagat ttagtccgcc agctggcgct taacggtaag acgcgcgcaa tacgggaaca
    25561 agcgatgaga aaacttgatg agttgtattg atccacaacg taatgtttct taactatttg
    25621 attaattggg gataatcatt cctggcagtg agtccccaat accttgatat attctgaatt
    25681 tttaatgaag cggcgtgttg cgatatctcc gtcaggggaa ttgatgcacc atagcgcaaa
    25741 ccgaattatc aaggattgat aatgacgctc taccagataa aaccgctctt tcagtcgctg
    25801 ttaaggccga cgatgttttg gctttataag caccacgtta cagcgaatca catcactctt
    25861 gctgcactgg cgctttctct tcttaccgga ttgctgttag tgttggtggc acaacccatc
    25921 ctctttttgc tattgcccat cgtgcttttt atccgtatgg cgctcaatgc actggatggc
    25981 atgttggcgc gtgagtgcga tcagcaaaca cgtctggggg cgattttgaa tgaaactggc
    26041 gatgttattt ccgatattgc gctctattta ccgtttttat ttttaccgga aagtaacgca
    26101 ttactcgtaa tactcatgtt attttgcacc atattgactg agttttgcgg tttactcgcg
    26161 cagacgatta acggtattcg cagttttgcc ggtccatttg gcaaaagcga tcgcgcgtta
    26221 atatttggtc tgtgggggct tgccgttgcc atttatccac agtggatgca atggaataat
    26281 cttttatgga gcattgcttc aattctgctt atctggactg cgattaatcg ttgtcggagt
    26341 gtgcttctta tgagcgctga aagataatgc tggaaaaatc tctggcaaca cttttcgcgt
    26401 tgttaatttt agcaacgctg ataaaccgtt ttctgctatg gcggttaccg gagagaaaag
    26461 gggatgaagt tacattacgt attcgtacct ggtggggtat cgtcatttgt ttttcactgg
    26521 tgatttcagg tccacgctgg atgacgttga cgttttttgc gctgataagt tttctggcat
    26581 tgaaagaata ttgtacgctt atatccatac attttccgcg ttggctatat tgggttattc
    26641 ctcttaacta tttgcttatc ggttttaatt gctttgagct gtttctgtta tttatacctt
    26701 tggctgggtt tctaatatta gccaccgggc aagtttttgt gggggacccc tccggttttc
    26761 tgcataccgt gagcgccatt ttttggggct ggataatgac cgttttcgcc ttgagtcatg
    26821 ccgcctggtt attaatgttg ccaaccacaa atatccaggg cggggcgtta ctggtgttat
    26881 ttcttttggc attaaccgag tcaaacgata ttgcacagta tttatggggg aaatcctgcg
    26941 gcagaagaaa agtggtcccc aaagtcagcc cgggaaaaac gctggaaggt ctggtggggg
    27001 gcgtaatcac caccatgatt gcgtcactga ttattggacc gttgctgaca ccgctaaata
    27061 cattacaggc attattagcg ggtttgttaa ttggtattag tggtttttgc ggcgatgtcg
    27121 tgatgtcagc catcaaacga gatattggtg ttaaagatag tgggaaacta ttaccaggac
    27181 atggcggact ccttgacagg attgactcat taattttcac cgctccggta tttttttatt
    27241 ttattcgcta ctgctgttac tgaaggaaaa tagagaaaat ggaaaattca cgcatccctg
    27301 gggaacattt ttttaccagc agtgataata cagcgttgtt ttatcggcac tggcccgctt
    27361 tacagcccgg ggctaaaaag gtcatcgtct tatttcatcg cggtcatgaa cattctggtc
    27421 gtctacaaca tctcgttgat gaactggcga tgccagatac tgttttttat gcctgggatg
    27481 cccgagggca tggacaaact tcggggccgc gtggttatag cccatctctt gcgcgttcag
    27541 tgcaggatgt cgatgaattt gttcgttttg ctgccagcga tagccaggtc ggactggatg
    27601 aggtggtagt gatcgcgcaa agcgtcggcg cagtgctggt tgccacatgg gttcatgatt
    27661 atgcgcctgc aattcgcggg ctggtgctgg cttctccggc ctttaaggtt aaattgtatg
    27721 taccgctggc acgtcctgcg ctggcgttat ggcatcgtct gcgtggtctg ttttttatta
    27781 attcctatgt gaaaggacgc tatttgaccc acgatcggca acgggtggcg agtttcaata
    27841 atgatccgct gatcacacgg gcgattgccg ttaatatctt gctcgatctc tacaaaacgt
    27901 ctgaacgtat tattagagat gcggcggcga ttacgctccc cacgcaactt ctgatatcag
    27961 gcgatgacta tgtggtgcat cgccaaccgc agattgattt ttatcagaga ttacgtagcc
    28021 ctctgaaaga gctgcatctg ctgccaggct tttatcacga cacgttgggt gaagagaaca
    28081 gggcgcaggc atttgaaaaa atgcaaagct ttattagtcg tttatatgct aacaagtcac
    28141 aaaaatttga ttatcagcat gaagaccgca ctggaccatc agcggatcgc tggcggctcc
    28201 tttcaggtgg acccgtgcca ttatcgccgg ttgatttggc gtatcgcttt atgcgaaagg
    28261 cgatgaaatt gttcggggcg cactctgcgg gcctgcatct cggaatgagc accggctttg
    28321 attcaggcag ttccctggat tatgtctatc aaaatcaacc gcaaggcagt aacgcattcg
    28381 ggcgcttaat tgacaaaatc tacctgaaca gtgttggctg gcgcggtatt cgccagcgta
    28441 aaacccattt acaaatactg attaaacaag ccgttgccga tctccacgcc aaaggtttag
    28501 ccgtccgcgt ggttgacatt gccgcagggc atgggcgcta tgtactggat gcgctggcaa
    28561 acgagcctgc cgtaagcgat attttgttac gtgattacag tgagttaaat gttgcacagg
    28621 ggcaagcgat gattgcccaa cggggaatgt ctgggcgggt gcgttttgaa cagggcgatg
    28681 cgtttaaccc ggaggaactc agcgcgttaa ctccgcggcc tacgctggcg attgtctctg
    28741 gcctgtatga gctttttccc gaaaatgagc aggtaaaaaa ctcactcgca ggtcttgcca
    28801 atgccatcga accgggcggc attctcatct acaccgggca gccgtggcac cctcaactgg
    28861 agatgattgc cggggtgtta accagtcata aagatggtaa accgtgggta atgcgcgtgc
    28921 gttcgcaagg ggagatggat tcactcgtgc gtgatgccgg atttgataaa tgcacacaac
    28981 ggattgatga gtggggtatt tttacggttt cgatggcggt gcgtcgtgat aactgaacgt
    29041 cggaacgtat tgctacaagg cgctggctgg ttattgttgc tggccccgtt tttcttcttc
    29101 acctatggat ctcttaatca gttcaccgcg gttcaggacc ttaacagcca tgatatcccc
    29161 agtcaggtat tcggttggga aacggcgatc ccttttcttc cctggactat tgttccttac
    29221 tggagtctgg atcttttata tggattttcg ctgttcgttt gtagcacgac attcgaacag
    29281 cgccgacttg tccaccggct tattctggca acggtaatgg cctgctgcgg ttttttgctc
    29341 tatccgctga agtttagttt tatccgtcct gaagtgagtg gggtgacggg atggctattt
    29401 tcgcaacttg aactgtttga tctgccttat aaccagtctc cttcgctgca tattattctc
    29461 tgctggctac tttggcgtca ctttcgtcag catctggctg tgaggtggcg taaagtctgc
    29521 ggcggatggt ttttactcat cgccatttcg acgctgacga catggcagca tcattttatt
    29581 gatgtcatca cagggctggc ggtaggtatg ttgattgact ggatggtgcc cgtcgaccgt
    29641 cgttggaatt atcagaaacc tgatcaacgt cgaatcaaaa tagcactgcc atatgtcgta
    29701 ggcgcgggct cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
    29761 gtctggttat gttggccagt attatcgcta ctcattattg gccgggggta cggtgggctt
    29821 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
    29881 acattgccct ggcgcattgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
    29941 ccggtgagca aaatgactgc tggtgtttat ttaggggcgt ttccacgaca tattccggca
    30001 cagaatgcgg ttctggacgt cacctttgaa ttccctcgcg gacgagcgac aaaagatcga
    30061 ctctatttct gtgtaccgat gctggatctg gtggttccgg aagaggggga gctccgacag
    30121 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgcg
    30181 ttgggattat cgcgcagtgc gctggtagtg gcggcatggt tgttatgtta cggacactgt
    30241 aaaaccgttg atgaagcgat tagttatatt cgagccagac gttcgcatat tgtgcttaag
    30301 gaagatcaca aagcgatgct gaaattatgg gaaaacaggt aagtggattg agatgtggac
    30361 tgaactacag tccacatcaa gatcgtgtcc ggttatgcag caacaatgct gtcgatggcc
    30421 gctttcgcgt cagactgcgc tttcgctgcc atttccggac cgtatgcgat cccttcggcg
    30481 aagacaaatt tcacatcggt aatgccgata aagccgagga acgtggacag atacggcgtc
    30541 accaggtccg ttggtccatc tttgtggatc ccaccgcggc tggtaataac gatggctttt
    30601 ttacccgtta ccagaccttc cggaccgttc tcggtatagc ggaaagtaac gcctgcgcgt
    30661 gcaaccaggt caaaatagtt tttcaactga gttgagatgt tgaagttata catcggtgcc
    30721 gcaataacga taacgtcgtg ggctttcagc tcggcaatca gctcatcgga aagcgccaga
    30781 gcttcctgct gacgcggagt cagcggcgca tcgctcggac gcagtgcacc aaccagttcg
    30841 ccatccagta ccggaatcgg atttgcagcc aggtcgcgaa cggtgatttc atcagcggag
    30901 tgcttttcgc gccattgttc aacaaaataa tcggacaact gattagactg agagtaccct
    30961 gccaggatgc tggatttaag aactaatacc ttgctcatgg tgtttcctta tagatgtttg
    31021 aatgggcgat gccccgttgc ttgctgacac tttattcaca atcctgccac agagatagcg
    31081 caataaatcg aagcctatgt tcgaatttat tgaacaacgc atagaaagcc gcgatgtggt
    31141 actctatatc tatcatttaa aagaaaatta atcaggcaga ctactgccca ctaacgttat
    31201 gacagaacaa caaaaattga cctttacggc cttgcagcag cggctggatt cgctgatgct
    31261 gcgcgacaga ctgcgttttt ctcggcgtct gcacggtgtt aagaaggtta aaaatcctga
    31321 tgcacaacag gccattttcc aggagatggc gaaagagatt gaccaggcgg cagggaaagt
    31381 cctgctgcgt gaagcggcac gaccggaaat tacttatcct gacaatttac cggttagtca
    31441 gaaaaaacag gacattctcg aagcgattcg tgatcaccag gtggtgatcg tcgccgggga
    31501 aacgggttct ggtaaaacga ctcagttacc gaaaatctgt atggagctgg ggcgcgggat
    31561 taaagggctg atcggccata cccagccgcg tcgcctggcg gcaagaacgg tggcgaaccg
    31621 tattgcggaa gagctgaaaa cggagccggg cggttgcatc ggttacaaag tgcgtttcag
    31681 cgatcacgtt agtgataaca cgatggtcaa gctgatgacc gacggtatcc tgctggcgga
    31741 gatccagcaa gaccgcctgc tgatgcagta cgacaccatc attattgacg aagcacacga
    31801 acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa gagttgctgc cgcggcgtcc
    31861 tgacctaaaa atcattatca cttccgcgac tatcgacccg gaacgctttt cgcgccactt
    31921 taataatgcg ccgattatcg aagtctccgg tcggacctat ccggtggaag tgcgctatcg
    31981 accgattgtt gaagaagccg acgacaccga gcgcgatcag ttgcaggcga tttttgacgc
    32041 cgtagacgaa ctgagtcagg aaagccctgg cgacattctg atctttatga gtggtgagcg
    32101 ggaaattcgc gataccgccg atgcgctgaa caagctaaat ctgcgtcata ccgaaatctt
    32161 gccgctttat gcgcggcttt cgaacagcga acagaaccgc gtgttccagt cgcacagcgg
    32221 acggcgcatt gtgctggcga ccaacgtcgc ggaaacctcg ctgaccgtac cggggattaa
    32281 atacgttatc gaccccggta cagcacgtat cagccgctac agctatcgca ccaaagtgca
    32341 gcgtttgccg attgaaccca tttcgcaggc gtctgccaat cagcgtaaag gccgctgtgg
    32401 gcgtgtgtcc gaagggatct gtattcgtct ctattccgaa gacgatttcc tttcgcgccc
    32461 ggagtttacc gatccggaga ttctgcgtac caacctggcc tcggttattt tgcagatgac
    32521 cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc gaagcaccgg ataaacgcaa
    32581 tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg atcaccacgg atgaacaggc
    32641 cagcgcctat aaactgacgc cgctcggtcg ccagctctcg cagttgcctg tcgatccacg
    32701 tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc gtgcgtgagg cgatgattat
    32761 cacgtccgcg ctttccattc aggatccgcg cgagcgtccg atggataaac agcaggcatc
    32821 ggacgaaaaa catcgtcgct tccacgacaa agagtctgac ttcctcgcgt ttgtgaatct
    32881 gtggaattat cttggcgagc agcaaaaggc gctttcttcc aacgccttcc gtcgcctgtg
    32941 tcgtaccgat tatctcaact atctgcgcgt gcgcgaatgg caggatatct acacccagtt
    33001 gcgccaggtg gtgaaagaac ttggcattcc ggttaacagc gaaccggcgg agtatcgcga
    33061 aattcacatt gcgttgctga cgggtttact ttcccatatc ggcatgaaag atgccgataa
    33121 acaagaatat accggcgcac gtaacgcgcg tttctccatc tttcccggtt ctggtttatt
    33181 caaaaaaccg ccgaaatggg taatggtggc ggaactggta gaaaccagcc gcctgtgggg
    33241 gcgcattgct gcgcgtatcg acccggaatg ggtggagcct gtcgctcagc atttgattaa
    33301 acgcacctac agcgaacctc actgggaacg ggcgcagggc gcggtgatgg caacggaaaa
    33361 agtcactgtt tatggtttgc cgattgttgc cgcgcgcaag gtcaactaca gccagatcga
    33421 tccggcgtta tgccgtgaac tctttattcg ccatgcgctg gtggaaggtg actggcagac
    33481 gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc gaagtggaag agctggaaca
    33541 caaatcacgt cgccgcgata ttctggttga tgacgaaacg ttgtttgagt tctacgacca
    33601 gcgcatcagc cacgatgtaa tctccgctcg tcacttcgac agctggtgga aaaaagtcag
    33661 ccgcgaaacc cctgatttgc tcaactttga aaaaagcatg ttgatcaaag agggcgcaga
    33721 aaaaatcagc aagctggatt acccgaactt ctggcatcag ggcaatctca agctgcgttt
    33781 gagctatcag tttgagcccg gcgcggatgc tgacggtgtg accgtacata ttccgctgcc
    33841 gttacttaac caggttgagg aaagcgggtt tgaatggcag atccccggtc tgcgccgcga
    33901 actggtgatt gctctgatta aatcgttgcc gaaaccggta cgccgtaatt ttgtacccgc
    33961 gccaaactat gccgaagcgt ttttaggccg cgtcaaaccg ctggagttac cgttgctcga
    34021 cagccttgag cgcgagttac ggcggatgac cggcgttacc gttgaccgcg aagactggca
    34081 ctgggatcag gtgcctgatc acctgaaaat taccttccgc gtggtggatg acaaaaacaa
    34141 gaagctaaaa gaagggcgct cgctacaaga tctgaaagat gcgctgaaag gcaaagtgca
    34201 ggaaacgcta tctgcggtgg cggatgacgg tatcgagcag agcggcttac atatctggag
    34261 ttttggtcag ctgccggaaa gctacgaaca gaagcgtggc aactacaaag tgaaggcgtg
    34321 gccggcgctg gtggatgagc gcgacagtgt ggcgatcaaa ctgtttgata acccgctgga
    34381 gcaaaagcag gcaatgtgga acggtcttcg ccgtctactg ctgctgaata ttccatcgcc
    34441 aatcaaatat ttacatgaaa agttaccgaa caaagccaag ctgggactgt actttaaccc
    34501 gtatggcaaa gtgctggagc tgatcgacga ctgtatctcc tgcggtgtgg ataaattgat
    34561 cgacgccaat ggtggccctg tctggacgga agaaggcttt gctgcgctgc atgaaaaagt
    34621 gcgtgccgaa ctgaacgaca cggtggtgga tattgcgaag caggtcgagc aaatccttac
    34681 ggcagtgttc aatatcaaca aacgtctgaa agggcgggtg gatatgacca tggcgctggg
    34741 gctttctgac attaaagcgc agatgggcgg gttggtatat cgcggttttg tcactggtaa
    34801 cggcttcaaa cggctgggcg acacgctgcg atatttgcag gcgattgaaa aacggctgga
    34861 aaaactggcg gttgatccac atcgcgaccg tgcgcagatg ctgaaagtcg aaaacgtcca
    34921 gcaggcgtgg cagcaatgga tcaacaaact gccgcccgca cgtcgtgagg atgaagacgt
    34981 gaaagagatc cgttggatga tagaagagtt gcgcgttagt tacttcgctc aacaacttgg
    35041 tacgccttat cctatttcgg ataagcgtat tttgcaggcg atggagcaga ttagcggtta
    35101 acactgctat ttgcctgata aagaaaaaac ccggtaagca tttagcgccg ggttttttat
    35161 taattctaaa acggtaaggg taaaaattca ggaattcaga aaaatacaat tctctgctgc
    35221 aagatgaata atgtttatct acagcatttc cttaaaagat atgtcaggct tgcggagtgg
    35281 cggttaagga aatacgactt cctcctttca gagtgctccg cttctcacta ttatctcacg
    35341 cagtattctt aagggaacga taaggaggaa ccatgaacat taccccgttt ccgacgcttt
    35401 cgccggcaac tatagatgcc ataaatgtta tcggacagtg gctggcgcag gatgatttct
    35461 ccggtgaggt gccgtatcag gccgattgcg tgatccttgc aggcaatgcg gttatgccga
    35521 ctatcgacgc ggcatgtaag attgctcgcg atcagcaaat tcctttactg attagtggtg
    35581 gtatcggtca ctcgacaact tttttgtata gcgccatcgc acagcatccg cactacaaca
    35641 ctatccgcac cactggcaga gcagaagcga ccatcctggc ggatatcgct catcagttct
    35701 ggcacattcc gcatgaaaaa atctggattg aagaccagtc aacaaactgc ggtgaaaacg
    35761 cacgctttag catcgcgcta ttgaatcagg ccgtagaacg agttcatacg gctatcgttg
    35821 ttcaggaccc caccatgcag cggcgcacga tggcgacgtt ccgccgtatg actggggaca
    35881 atcccgatgc accacgctgg ttaagttatc ccggattcgt tcctcagtta ggaaataacg
    35941 cagacagtgt aatctttatt aatcagttac aaggattatg gccagttgag cgttatctct
    36001 cactactcac tggcgagctg ccgcgtttac gcgatgatag cgatggctac ggtccccgcg
    36061 ggcgagattt tatcgttcac gttgattttc cggcagaagt catccatgca tggcaaacgc
    36121 tgaaacatga tgcggtgctc atcgaggcga tggaaagtcg ctcgttacgt taaaaattgc
    36181 ccgtttgtga accacttgtt tgcaaacggg catgactcct gacttttatt tctgcctttt
    36241 attcctttta cacttgtttt tatgaagccc ttcacagaat tgtcctttca cgatgccgtc
    36301 tctctgatga ttgatgttaa ttaacaattc attcaccgaa aacaaacata taaatcacag
    36361 gagtcgccca tgtcagtacc cgttcaacat cctatgtata tcgatggaca gtttgttacc
    36421 tggcgtggag acgcatggat tgatgtggta aaccctgcta cagaggctgt catttcccgc
    36481 atacccgatg gtcaggccga ggatgcccgt aaggcaatcg atgcagcaga acgtgcacaa
    36541 ccagaatggg aagcgttgcc tgctattgaa cgcgccagtt ggttgcgcaa aatctccgcc
    36601 gggatccgcg aacgcgccag tgaaatcagt gcgctgattg ttgaagaagg gggcaagatc
    36661 cagcagctgg ctgaagtcga agtggctttt actgccgact atatcgatta catggcggag
    36721 tgggcacggc gttacgaggg cgagattatt caaagcgatc gtccaggaga aaatattctt
    36781 ttgtttaaac gtgcgcttgg tgtgactacc ggcattctgc cgtggaactt cccgttcttc
    36841 ctcattgccc gcaaaatggc tcccgctctt ttgaccggta ataccatcgt cattaaacct
    36901 agtgaattta cgccaaacaa tgcgattgca ttcgccaaaa tcgtcgatga aataggcctt
    36961 ccgcgcggcg tgtttaacct tgtactgggg cgtggtgaaa ccgttgggca agaactggcg
    37021 ggtaacccaa aggtcgcaat ggtcagtatg acaggcagcg tctctgcagg tgagaagatc
    37081 atggcgactg cggcgaaaaa catcaccaaa gtgtgtctgg aattgggggg taaagcacca
    37141 gctatcgtaa tggacgatgc cgatcttgaa ctggcagtca aagccatcgt tgattcacgc
    37201 gtcattaata gtgggcaagt gtgtaactgt gcagaacgtg tttatgtaca gaaaggcatt
    37261 tatgatcagt tcgtcaatcg gctgggtgaa gcgatgcagg cggttcaatt tggtaacccc
    37321 gctgaacgca acgacattgc gatggggccg ttgattaacg ccgcggcgct ggaaagggtt
    37381 gagcaaaaag tggcgcgcgc agtagaagaa ggggcgagag tggcgttggg tggcaaagcg
    37441 gtagagggga aaggatatta ttatccgccg acattgctgc tggatgttcg ccaggaaatg
    37501 tcgattatgc atgaggaaac ctttggcccg gttctgccgg tggtcgcatt tgacacgctg
    37561 gaagatgcta tctcaatggc taatgacagt gattacggcc tgacctcatc aatctatacc
    37621 caaaatctga acgtcgcgat gaaagccatt aaagggctga agtttggtga aacttacatc
    37681 aaccgtgaaa acttcgaagc tatgcaaggc ttccacgccg gatggcgtaa atccggtatt
    37741 ggcggcgcag atggtaaaca tggcctgcat gaatatctgc agacccaggt ggtttattta
    37801 cagtcgtaat gagtaaaaga ggcggaggtt ttttcctccg cctgtgcgcg tcagagttta
    37861 gcgaattttt cgagggtgcg aataagctgc gtgacgaagc catattcgtt atcgtaccag
    37921 gcgaccgttt tcaccagttg taaatcgccc acggcggtaa tttccgtttg cgtggcatca
    37981 aacaccgaac cgaaatggct gccaatgata tcggaagaga ctatttcttc atcggtataa
    38041 ccaaatgact cgttattggt ggttgcttgt ttaagtgcgt tattcacctc ttcggcagtc
    38101 actttttttc cgagaatgga caccagctca gtgaccgaac ctgttttcac cggcacgcgt
    38161 tgcgcatgac ctttcagttt gccgctcagt tccgggatca ccagaccaat ggcttttgcc
    38221 gcccccgtag tgtggggaat gatattttct gccgctgcgc gtgaagcacg taaatcttta
    38281 ccacgtgggc catccaccag tgactgggtg ccagtatagg catgaatggt cgtcatcgtg
    38341 ccgacttcta tcccgaaact gtcatgcaag gctttggcca tcggcgcaag acagttagtg
    38401 gtgcatgacg ccacggaaac aatggtgtcg ttgccatcca gagtgtcgtc attgacgtta
    38461 taaacgatag ttttcatttc accggcaggg gcggaaatca acaccttctt cgcaccagca
    38521 tcaagatgcg cctgcgattt ctcggcggag gtataaaagc cagtacattc gacaatgatt
    38581 tctgcacctt tcgctttcca cggaatattt ttagcctctt tttcggcgta aaccgcgata
    38641 cttttcccat caacgataag tgaatcttcc gtaaaatcaa cgctccaggg gaatggtccg
    38701 tagtttgaat catgtttcag caggtaggcg agaatttttg gggaagtgag atcattaata
    38761 gcgacaacgt ctatgttgct tttgacttca agtaatcgac gcaacaccag tcgaccgata
    38821 cgaccaaaac cgttaatacc aactttactc atggttttct cctgtcagga acattcggat
    38881 gaaaattgat cctttccagg cttagaccag gattgtggga tgggcaatct ccattcttac
    38941 agtgaaacgt aacgtactga aaacgggtga acaatattta atgaaatttt gagaaaagcc
    39001 cgttatgtta acggaaaatt atgttaaagc aggaaatgtt atggaaaata aatactcaag
    39061 gttacaaatc agcattcact ggctggtctt tttgctggtt atcgcagcgt attgcgcaat
    39121 ggagtttcgt ggtttcttcc cacgtagcga tcggccactc atcaacatgg ttcatgtttc
    39181 ctgtggtatc tcaattctcg tgctgatggt ggttcgtctt ttgttaagac tgaaataccc
    39241 aaccccgccg attataccta agccaaaacc gatgatgacg ggactggcgc atttgggaca
    39301 tttggtgatt tatctcctgt ttattgcgct gccagtgatt ggtttggtga tgatgtataa
    39361 ccggggcaac ccgtggtttg cgtttggttt gacgatgcct tacgcttcag aggccaattt
    39421 cgaacgggtt gatagcttaa agtcgtggca tgagacgctg gcgaatctgg gatattttgt
    39481 catcgggttg cacgctgcgg ctgcactggc acaccactat ttctggaagg acaacacact
    39541 tctacgcatg atgccgcgta aacgttcctg aaggatattt aaagaaaacg cctgtactaa
    39601 aaccgacccg tggtacaggc gaagaatacg ggtctacatc ggaagcgcct attatattta
    39661 tttgtatgac aaataaaacc ataatccttg cccatacgtc catctggctt atttttaatc
    39721 aattcacccg atctttgatc tcatcaacgg tatcaaaata aaccagcgta taacgttcaa
    39781 atcgaacata agattcgata aaccatggag gttatatgaa aaaactggca cttattttgt
    39841 ttatgggaac gcttgtttcc ttttatgccg atgccgggcg caaaccctgt tctggttcga
    39901 aaggggggat ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat ggttctatta
    39961 gtgcctcgaa aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat
    40021 ctgctagtca gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca
    40081 tgagcgcagc aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg
    40141 tcaggagtgt gaatgtcaga atcgtaatgc agataatgag cagacacacc accagagggt
    40201 tgtgcttcat agcctttctc cttgccggat ggcgggtaag aggctaagat ctgaattgct
    40261 aggttcattc gttggcctcg gttgatagaa atatcggtcg gggccttcgt ctttctgatt
    40321 cccggttagc ctgaaaacag aaagtctcag gcacccgcag gcatcctatg aggtttcctt
    40381 agggacgaaa ataatcactt cacgaaattg cgtgctgttt tccagaattt ttcgtcattc
    40441 gggttagcca gtttagccat tcgttactct cttcattcca atagcattaa ttttctctgc
    40501 aataattgtt gtaaaaatgt gacgcaaaga ggtttttggt cataagtaat taccgtcaag
    40561 tgccgatgac tttctatcag gagtaaacct ggacgagaga caacggtaat gaatacaact
    40621 ccctcacatc gattaggttt tttgcatcac atcaggctgg ttccgttatt tgcctgcatt
    40681 cttggcggta tcttagttct attcgcatta agttctgctc tggctggcta tttcctctgg
    40741 caggccgatc gcgatcagcg tgatgttact gcggagattg agattcggac cgggttagcg
    40801 aacagttcag attttttgcg ttcagcccgg atcaatatga ttcaggccgg ggctgcaagt
    40861 cgtattgcgg aaatggaagc aatgaagcga aatattgcgc aagccgaatc ggagattaaa
    40921 cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa
    40981 gccctcgaca ctgaattaaa tcaacgcttt caggcttata tcacgggtat gcaacctatg
    41041 ttgaaatatg ccaaaaatgg catgtttgaa gcgattatca atcatgaaag tgagcagatc
    41101 cgaccgctgg ataatgctta taccgatatt ttgaacaaag ccgttaagat acgtagcacc
    41161 agagccaacc aactggcgga actggcccat cagcgcaccc gcctgggtgg gatgttcatg
    41221 attggcgcgt ttgtgcttgc cctggtcatg acgctgataa catttatggt gctacgtcgg
    41281 atcgtcattc gtccactgca acatgccgca caacggattg aaaaaatcgc cagtggcgat
    41341 ctgacgatga atgatgaacc ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta
    41401 cagcaaatgc agcattcact ggggatgaca gtagggactg ttcgacaggg tgcggaagag
    41461 atttatcgtg gcaccagcga aatttcagct ggcaatgcgg acctgtcatc tcgcaccgaa
    41521 gaacaagcgg cggctatcga acaaactgcc gctagcatgg agcaactcac tgcgacggtg
    41581 aaacagaatg cggataacgc gcatcatgcc agcaaactgg cgcaagaggc ttctattaaa
    41641 gccagcgatg gcgggcagac ggtttccggt gtagtaaaaa cgatgggcgc tatctctaca
    41701 agttcgaaga aaatttccga gatcaccgcc gtcatcaaca gtattgcttt ccagacgaat
    41761 attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg gtgagcaagg ccgtggattt
    41821 gccgttgttg ccagcgaagt acggacactc gcaagccgca gcgcccaagc ggcgaaagag
    41881 attgaaggct tgatcagtga atcagtcagg ttaattgacc tggggtcgga tgaggtggca
    41941 acggcaggga aaaccatgag cactattgtt gatgccgtcg cgagtgtcac acatatcatg
    42001 caggaaatcg ccgccgcctc ggatgaacaa agtagaggca taacgcaggt tagccaggcg
    42061 atttctgaaa tggataaggt gacgcaacag aatgcttctc tggtagaaga ggcctcagcg
    42121 gcggcggtgt cccttgaaga acaggcggca cgattaactg aggcggtgga tgtattccgt
    42181 ctgcacaaac attctgtgtc ggcagaacct cgcggagcgg gtgaaccagt tagtttcgct
    42241 acggtgtgaa aatgttcaag gagggatcga cagatccctt cacctttcag aacggcattg
    42301 attttcgaat agcgttaatc atcaactggc aaccagaaga gaacgtcgca tctacgcggg
    42361 tcagtattcc aatcggttcg cctgcaccat gtcccggaac aggcagggcc accagcgtgg
    42421 catgacgcag gtcgtctttt acagcgccag aagggacaaa ccacacgtaa tcgtattcaa
    42481 ccgtaagttg acgagatagc gaagcagaca gcgtttcgat acaacccgaa ggaattttac
    42541 agccctggct ctgcactaat gcatctgaat gctggcgtgg cgcagtgcct tctggtgata
    42601 caacgaccgg ccattccagc acccggctta gcgttacgtt ctcctgaagt agcgggtgat
    42661 tagggcggac aaccagcttc aacgattcaa gaaacagcag ttcgtaatta agcccggtca
    42721 tcagttcagg atctgacatc cgaccaatgc cgatatcgat ttccccggtt tttaaacccg
    42781 ccagaatcat agggttactc attgtcgcaa cttgcaaggt cgtctctttt tgttgctgat
    42841 gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc agtaggtagt gcaccaaccc
    42901 tgacgacatc attattaaga ccttctttac gatgaagcga ctgtccggca gtgttgatgg
    42961 cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc
    43021 cctgacgacc acgctcaaac aagcgagcgc cagtcagctg ctccagttca ttcaatgtct
    43081 tagagagcgc aggttgactc aaattaaggg tttcagccgc gcgccccaaa gttccttgtt
    43141 gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat gcgctgacta aacagactat
    43201 ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat
    43261 tatgataact tgattgcaaa atattattaa caattaaagc aattatgtta cagcaaaatg
    43321 gataatattg atgttttcgc ggcgagatca cagtttgtaa attcttcccg ccagagtgaa
    43381 tgcggttacc tacactccag attactgacc actggaggca gacactatgg cgaacagcat
    43441 cacggcggat gagattcggg aacagttttc gcaggcaatg tcagccatgt accagcaaga
    43501 agttccgcaa tatggcacgc tgctggaact ggtagctgat gtgaatctgg ctgtgctgga
    43561 aaacaatcct caactgcacg aaaaaatggt aaatgcagac gagctggcgc gactgaatgt
    43621 tgaacgtcat ggggcgattc gcgttgggac tgcacaagag cttgctactc ttcggcggat
    43681 gtttgccatt atggggatgt acccggtgag ctattacgat ctctcgcagg caggggtgcc
    43741 ggtacattcg acagcatttc ggcccattga tgatgcttct ctggcgcgta atcccttccg
    43801 cgtttttacc tccttactcc gccttgagct tatcgagaac gaaattttgc gccagaaagc
    43861 ggcggagatt ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac tgttagagga
    43921 atatgagcag cagggcggtt ttaacgaaac acaggcacag gagtttgtgc aggaagccct
    43981 ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa gaaacctatc gcgccttgca
    44041 caacgaacat cggttgattg ctgatgtggt ctgttttcct ggatgccata tcaaccacct
    44101 gacgccacgt acgctggata ttgaccgggt gcagtcgatg atgcctgaat gcggaattga
    44161 acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta ccgattttac tacgccagac
    44221 cagctttaaa gcactggaag agacggtgtt gtttgcgggg cagaaacagg gcacgcatac
    44281 cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag ggcgacaact
    44341 gtatgatgat cttctgcgta acgctggaac cgggcaggat aatctcactc accaaatgca
    44401 tttacaggaa accttccgca cttttcctga cagtgagttt ttaatgcgtc agcaagggct
    44461 ggcatggttc cggtaccgtc tgacgccttc aggtgaggcg catcgtcagg cgattcatcc
    44521 cggagacgat ccacagccct taattgaacg tggttgggtc gcggcgcaac ccattaccta
    44581 tgaagatttc ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac
    44641 gcagacacgc agtcacggta atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc
    44701 ggttttggat gagttccagc tttaccagga agcggaagaa cgcagtaaac gtcgctgtgg
    44761 tttgctttaa attctgacca tccgcctttg caaaaatttg cctgatttta caaacgaatc
    44821 aggctcatcc catcgacata aaaaaaatgc cgatttatgc atattctctc agttcaacaa
    44881 ttggattatt aataaatatt gtctagagtg agcggtcata aataagcact ttcttgccgc
    44941 tgaaaacgac cagcgcggga ccattcacaa caccagaagg actcactttc aggtatggat
    45001 cgtagacgat ttattaaagg ttcaatggct atggccgccg tgtgcggtac cagcggcatt
    45061 gcttctcttt tttctcaggc ggcattcgcg gcagattctg atattgccga cgggcaaacc
    45121 cagcgttttg acttctccat tctacagtca atggcgcacg acttagcgca aacagcgtgg
    45181 cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca ggcttataac
    45241 agtattcaat acgacgccga aaaatcgctc tggcataacg ttgagaaccg tcaactggac
    45301 gctcagttct tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat
    45361 ccagcaacac atctggcgcg tgaaattcac tttcgcccgg agttgttcaa atacaacgat
    45421 gcgggtgttg atacaaaaca attagaaggg caaagcgatc tcggctttgc cggttttcgc
    45481 gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat catttctcgg cgcgagttat
    45541 ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg cccgcggcct ggcgatcgac
    45601 acttacactg acagtaaaga agagttcccc gactttaccg ccttctggtt tgatacggta
    45661 aaaccggggg caactacctt taccgtttat gcgttgctcg atagcgccag cattactggt
    45721 gcctataagt tcactatcca ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac
    45781 ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc cgatgaccag tatgttcagc
    45841 tgcggtacta atgaacgtcg gatgtgcgat acaattcatc cgcaaattca tgactctgat
    45901 cgtctgtcca tgtggcgggg caacggcgag tggatttgcc gtccgctgaa taatccgcaa
    45961 aaattgcagt tcaatgctta caccgacaac aacccgaaag ggtttggttt attgcaactg
    46021 gatcgtgact tctcccatta tcaggacatt atgggctggt ataacaaacg cccaagtctg
    46081 tgggtggagc cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga aatcccaaca
    46141 acgggcgaaa cgctggataa cattgtctgc ttctggcagc cagaaaaagc tgtaaaagca
    46201 ggtgatgagt ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc
    46261 ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga aggttgggca
    46321 ccaggtgaac actatcccga aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt
    46381 gatctgaaag ctgccgcacc aaaaggcatt gagccggtga ttacgctttc cagtggggaa
    46441 gcgaagcaaa tcgaaattct ctatattgaa cccattgatg gttatcgtat tcagtttgac
    46501 tggtatccga cttcggactc cactgatccg gtcgatatgc ggatgtatct gcgttgtcag
    46561 ggcgacgcta tcagtgaaac atggctgtat cagtatttcc cgccagcgcc ggataaacgt
    46621 cagtatgttg acgaccgcgt gatgagttaa tcgttttttc ttcggcacct tcttcgggag
    46681 gtgccgtctg gttaaacacg atcccgctcg catttttccc taagttaaat gagtaatctg
    46741 atggtgtgta tttcagatac accttgtcag ccactaacag ggagtgcgta tgtttccaga
    46801 ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga
    46861 taaacacaat aaacttgatc atgaaattgc cagaaaggaa ggttccgacg gtcgagggta
    46921 caatgcggaa gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa
    46981 aatcctgcag caggagagcg tcaaagaggt gtaaactttc ctaagccgcc agccaggcgg
    47041 cttttttaac aacagcatgg attgactgga gataagatga ctgaaacgat aaaagtaagc
    47101 gaatcacttg aattacatgc tgttgcggaa agtcacgtca cacctcttta tcagttaatc
    47161 tgtaaaaata aaacctggtt acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa
    47221 gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa
    47281 atgttcatga ttttcaaaga agatgaactt atcggcgtta tctcgtttaa tcgtattgaa
    47341 ccactgaata aaactgctga aatcggctac tggctggacg aatctcatca ggggcagggg
    47401 atcatttctc aggcgctgca ggcattgatt catcattacg ctcagtctgg tgaacttaga
    47461 cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat
    47521 ggttttatcc ttgaaggttg cctgaaacag gctgagttcc tgaatgatgt ctatgatgat
    47581 gtgaacttat acgcgcgtat tatcgattca taataaccct gccagcggcg ttcgcgtaat
    47641 gcgctcctcg ccgttaatga cttttggacc acggacatga accgtgtcgc catcaaaggc
    47701 ttcaacgacg gcgtggtcgg ttaactccac atggttttca attatcaccg cacctgaaat
    47761 acggctctca ccttgtatta caacgtgctc gtcgagcaga atcggtccac cacgtaccac
    47821 ggcatttccg ccaatcaaga catgatgttt taacacacaa ttaccttcca caatggcata
    47881 ttccgccacc tgcgaactgt aatgaatcgt cggaatggca tcttcttcta tgccagcttt
    47941 cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc cagacgttgt tctcttcatt
    48001 accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag cataccggac
    48061 gacggcatcg ccataaattt gtgcctggtg gacaatacgc gaggcgctaa cccttgcgcg
    48121 atcataaatt tggagcagga gttgatggtc gggcgttaag ccttgtgctg cgacgatcat
    48181 agaatgttga ttaattaagg cgtgaccaaa tattctacat tgcccgcaaa ccagtgagtc
    48241 atgaatggtg acgctgtcac ttatatatgc accctggctg atttcactat tatcgatcca
    48301 gacattatcc gttgcgtaaa cctctcccca cagcacgctg gtgccggtga tgcgcgtatt
    48361 accggaaatc accgctccgc cgaacgcaat agcgttctgg tcgtaaatcc agcaatttcc
    48421 ttcctgtgct aacaccgttt cgcgatcgat ccagccgcca gcggttcctg ctatcacatc
    48481 gttaaaatcg ctaatggcga taatctgccg taataacaca ttttttttag tgccatcttc
    48541 ctgataacta aaggctcgct gttcttcact tagacgatat ttgcgca
//
LOCUS       DAMZII010000021        45043 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZII010000021 DAMZII010000000
VERSION     DAMZII010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.021"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            263..1033
                     /locus_tag="POA77_001165"
     CDS             263..1033
                     /locus_tag="POA77_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6364632.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1024..1497
                     /locus_tag="POA77_001166"
     CDS             1024..1497
                     /locus_tag="POA77_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDI6364633.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1605..2144
                     /gene="dnaT"
                     /locus_tag="POA77_001167"
     CDS             1605..2144
                     /gene="dnaT"
                     /locus_tag="POA77_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDI6364634.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2147..2884
                     /gene="dnaC"
                     /locus_tag="POA77_001168"
     CDS             2147..2884
                     /gene="dnaC"
                     /locus_tag="POA77_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDI6364635.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2933..3427
                     /locus_tag="POA77_001169"
     CDS             2933..3427
                     /locus_tag="POA77_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDI6364636.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3681..5972
                     /gene="opgB"
                     /locus_tag="POA77_001170"
     CDS             3681..5972
                     /gene="opgB"
                     /locus_tag="POA77_001170"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDI6364637.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6111..7133)
                     /gene="lgoD"
                     /locus_tag="POA77_001171"
     CDS             complement(6111..7133)
                     /gene="lgoD"
                     /locus_tag="POA77_001171"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDI6364638.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7272..8186
                     /locus_tag="POA77_001172"
     CDS             7272..8186
                     /locus_tag="POA77_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6364639.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVLVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8401..9762
                     /locus_tag="POA77_001173"
     CDS             8401..9762
                     /locus_tag="POA77_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364640.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9811..11475)
                     /gene="tsr"
                     /locus_tag="POA77_001174"
     CDS             complement(9811..11475)
                     /gene="tsr"
                     /locus_tag="POA77_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDI6364641.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11594..12040)
                     /gene="hpaR"
                     /locus_tag="POA77_001175"
     CDS             complement(11594..12040)
                     /gene="hpaR"
                     /locus_tag="POA77_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDI6364642.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12312..13601
                     /locus_tag="POA77_001176"
     CDS             12312..13601
                     /locus_tag="POA77_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDI6364643.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13598..15064
                     /gene="hpaE"
                     /locus_tag="POA77_001177"
     CDS             13598..15064
                     /gene="hpaE"
                     /locus_tag="POA77_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDI6364644.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15066..15917
                     /gene="hpaD"
                     /locus_tag="POA77_001178"
     CDS             15066..15917
                     /gene="hpaD"
                     /locus_tag="POA77_001178"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDI6364645.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15927..16307
                     /locus_tag="POA77_001179"
     CDS             15927..16307
                     /locus_tag="POA77_001179"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDI6364646.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16375..17178
                     /gene="hpaH"
                     /locus_tag="POA77_001180"
     CDS             16375..17178
                     /gene="hpaH"
                     /locus_tag="POA77_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDI6364647.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17189..17977
                     /gene="hpaI"
                     /locus_tag="POA77_001181"
     CDS             17189..17977
                     /gene="hpaI"
                     /locus_tag="POA77_001181"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDI6364648.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANAQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            18152..19528
                     /gene="hpaX"
                     /locus_tag="POA77_001182"
     CDS             18152..19528
                     /gene="hpaX"
                     /locus_tag="POA77_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDI6364649.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19538..20428
                     /gene="hpaA"
                     /locus_tag="POA77_001183"
     CDS             19538..20428
                     /gene="hpaA"
                     /locus_tag="POA77_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDI6364650.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20679..22241
                     /gene="hpaB"
                     /locus_tag="POA77_001184"
     CDS             20679..22241
                     /gene="hpaB"
                     /locus_tag="POA77_001184"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDI6364651.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22259..22771
                     /locus_tag="POA77_001185"
     CDS             22259..22771
                     /locus_tag="POA77_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDI6364652.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGNLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23164..25314
                     /gene="btsT"
                     /locus_tag="POA77_001186"
     CDS             23164..25314
                     /gene="btsT"
                     /locus_tag="POA77_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDI6364653.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25364..25567
                     /locus_tag="POA77_001187"
     CDS             25364..25567
                     /locus_tag="POA77_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI6364654.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25578..26534
                     /gene="yjiA"
                     /locus_tag="POA77_001188"
     CDS             25578..26534
                     /gene="yjiA"
                     /locus_tag="POA77_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDI6364655.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(26612..29152)
                     /locus_tag="POA77_001189"
     CDS             complement(26612..29152)
                     /locus_tag="POA77_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HDI6364656.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(29504..29668)
                     /locus_tag="POA77_001190"
     CDS             complement(29504..29668)
                     /locus_tag="POA77_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDI6364657.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            29845..31257
                     /locus_tag="POA77_001191"
     CDS             29845..31257
                     /locus_tag="POA77_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6364658.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(31499..32443)
                     /locus_tag="POA77_001192"
     CDS             complement(31499..32443)
                     /locus_tag="POA77_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6364659.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            32910..34142
                     /gene="mdtM"
                     /locus_tag="POA77_001193"
     CDS             32910..34142
                     /gene="mdtM"
                     /locus_tag="POA77_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDI6364660.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            34183..35463
                     /locus_tag="POA77_001194"
     CDS             34183..35463
                     /locus_tag="POA77_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI6364661.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRYAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            35579..36730
                     /locus_tag="POA77_001195"
     CDS             35579..36730
                     /locus_tag="POA77_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDI6364662.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            36740..37507
                     /locus_tag="POA77_001196"
     CDS             36740..37507
                     /locus_tag="POA77_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364663.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            37504..37761
                     /locus_tag="POA77_001197"
     CDS             37504..37761
                     /locus_tag="POA77_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDI6364664.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            37826..38685
                     /locus_tag="POA77_001198"
                     /pseudo
     CDS             37826..38685
                     /locus_tag="POA77_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
     gene            38753..39931
                     /locus_tag="POA77_001199"
     CDS             38753..39931
                     /locus_tag="POA77_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364665.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(39944..40498)
                     /gene="kptA"
                     /locus_tag="POA77_001200"
     CDS             complement(39944..40498)
                     /gene="kptA"
                     /locus_tag="POA77_001200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDI6364666.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            40748..41431
                     /locus_tag="POA77_001201"
     CDS             40748..41431
                     /locus_tag="POA77_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364667.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            41428..41889
                     /locus_tag="POA77_001202"
     CDS             41428..41889
                     /locus_tag="POA77_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDI6364668.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            41902..43074
                     /gene="iadA"
                     /locus_tag="POA77_001203"
     CDS             41902..43074
                     /gene="iadA"
                     /locus_tag="POA77_001203"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI6364669.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            43139..44050
                     /gene="hypT"
                     /locus_tag="POA77_001204"
                     /gene_synonym="qseD"
     CDS             43139..44050
                     /gene="hypT"
                     /locus_tag="POA77_001204"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDI6364670.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(44043..44435)
                     /gene="iraD"
                     /locus_tag="POA77_001205"
     CDS             complement(44043..44435)
                     /gene="iraD"
                     /locus_tag="POA77_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDI6364671.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(44432..44515)
                     /gene="idlP"
                     /locus_tag="POA77_001206"
     CDS             complement(44432..44515)
                     /gene="idlP"
                     /locus_tag="POA77_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDI6364672.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT        10568 a        11814 c        11510 g        11151 t
ORIGIN      
        1 ttaacattaa aaaacgtgcc cggttatcta ttatcctgat tcgtaaaggc caaacaataa
       61 attcattatg atgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      121 ttatcaccga gcttctcccg ataaccagga ttaatcagcg caattttgat cttaacctac
      181 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      241 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      301 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      361 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtt gaaagttcta tctcttcgaa
      421 cgccatagtg ctgacaacca ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      481 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttctggcgga
      541 gcaccatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg
      601 ttacccaaga tggctggtag ccttaatggt tggcctttct tgtgcctgtt tctgtaaact
      661 caataaaggt ggctgggatg gtgccgtcat caccttcttt gccagtacgg ccgcgatgta
      721 tatccgccag ctgctggcac aacgtcatct tcatccacaa atcaactttt gccttaccgc
      781 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
      841 caccattgcg atggccgcca gcgttctgct gctggtgccg ggatttccgt tgataaatgc
      901 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
      961 tctgctgaca ctggctacct gcgtcggcgt ggtgatggca ctgacgattt gggggctacg
     1021 cggatgggtg tgatcgagtt tctgtttgca ttggcgcagg atatgatcct cgccgccatt
     1081 ccagcggtcg gctttgcgat ggtgttcaac gttcccgttc gggcattacg ctggtgtgcg
     1141 ctgcttggcg cgattggtca tggttcacga atgatcttaa tgaccagcgg gctgaatatt
     1201 gagtggtcaa cctttatggc ttctatgctg gtcggcacca ttggtattca atggtcgcgc
     1261 tggtatctgg cgcatccgaa agtgtttacc gtggcggctg ttatccccat gttccctggc
     1321 atatcggctt ataccgcaat gatttcggcg gtaaaaatca gccagttagg ctacagcgag
     1381 tcattgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcatta
     1441 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1501 tgcctcgctt aaagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1561 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1621 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1681 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     1741 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagcg
     1801 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc tgctcccgtt gctgtaccca
     1861 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgactttatc cgcctggcgg
     1921 cgctatgggg cgtggcacta agagagccgg tgaccaccga agaactgacc tcattcattg
     1981 cctactggca ggcggaaggt aaagtctttc accatgtaca gtggcaacaa aaactggcgc
     2041 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2101 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2161 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2221 gggtgaagaa ctactggcct ggcagaaaga acaaggagcg atccgctccg ccgctctcga
     2281 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2341 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2401 aagcaaagcg cgccagtacg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2461 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2521 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2581 cttcaggaat agcggtacca gtgaagaaca actgcttaac gatctgagca acgttgatct
     2641 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     2701 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     2761 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     2821 cagtttgtgg gtgatcttca actgggatag ctaccgtagc cgggtaacag gtaaagagta
     2881 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     2941 aactgttaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3001 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3061 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3121 gaaccaggcc ttaagcgcag ataacatgaa caacgccgca ggcattctgc aatactgcgc
     3181 gaagcaaaag ctggcgtcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3241 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggatg gcattcaggg
     3301 tttgctgaaa accaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3361 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3421 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3481 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3541 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3601 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3661 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     3721 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acattaacgg
     3781 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     3841 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     3901 gcaaatacat cctgccgggt atcggcattg tgctggggct gacagcggtg ttcggtgcgc
     3961 tgggctggat cctgcgccgt cgtcgccatc atccgcacca ttttggttac agcctgctgg
     4021 cgctcttact ggcgctgggt tcggtggacg ccagcccagc atttcgtcag ataacggaac
     4081 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4141 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4201 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4261 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4321 gcatggtggc ttctcagtgc ggcataccgc tgtttgcccc ctttgaaggc aacgcctccg
     4381 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4441 gttatcagaa ctatttcgtg cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc
     4501 tgaagtcgca tggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtggccg
     4561 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4621 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4681 atacccatca cccggatggt tttatctcgc gtacctgtaa ccgcaaaaaa tatgattttg
     4741 acggtaagcc gaaccagtca ttcagcgcgg taagttgcag ccaggagaat atcgcggcgt
     4801 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg
     4861 accatttagc gatgaacaac acggcgtgga aataccttaa taagcaggat cgcaataacc
     4921 tgttttttgt cattcgtggc gacaagccgc agcaagagac gctggcagtg aagcgtaaca
     4981 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5041 gtcgtagcag tttatccggg cagtcaatgt cggaaatctt cctcaatatc aaagagaaaa
     5101 cattggcgtg gaagccggat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5161 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5221 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5281 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt
     5341 gctataagat ggcacaactc tgggctccgg aactggcact ctccaccgac tggtgtgtct
     5401 cgcaagggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggcagg
     5461 gcaaaacggc atttaaagat acggtcatcg acatggcgcg ttataaaggc aatgtcgata
     5521 cgctgaaaat tgttgataac gatattcgct acaaagccga cagtttcatc ttcaacgtcg
     5581 ccggtgcgcc agaagaggtg aaacagttta gtgggatttc ccgtccagag tcgtggggcc
     5641 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     5701 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     5761 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     5821 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     5881 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     5941 tggaaattaa agttgtggaa cgtgaagggt aatttctgac tgatgtgtag gtcgcataag
     6001 gtgcgaccta cacatcttct ccggcctaca aaacatcatt gagaaacaga aaaaaacagc
     6061 gcccaacact tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta
     6121 attacaccct taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6181 gtggcgaacg gatagcgatg ggttaacatc atgtcggcag tgatttttcc ttccgccatc
     6241 agtcgaccga ctttagcaaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
     6301 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag gccgacaaat
     6361 accaccgtgc cgccgtgacg aatcaaattc acggtgttat tcatcgcatg ttgattacct
     6421 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgtag ctgcgcgtca
     6481 aaatcttcgg ctgacgggtc cagtaaaggt aattccagac gcgttgccac atgttcacgg
     6541 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6601 gcgcccagac cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct
     6661 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgctgc cgcctgcggg
     6721 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     6781 ccatcctgat gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta
     6841 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     6901 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaaca
     6961 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     7021 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     7081 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7141 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7201 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7261 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7321 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7381 tgtacaacat cagccgtact acagtgcgtc atattctcag ccacttacgc gaatgcggcg
     7441 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7501 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7561 cgatgataaa ccagcgccag ttacgcccag gggaaacgtt ttctgaactg caactggctc
     7621 gggccgcggg tgtcagcccg gttctagtaa gagaatatct tttaaaattc ggccgttata
     7681 atcttattca cagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     7741 ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     7801 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc
     7861 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     7921 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     7981 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     8041 ttgccgtcga tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     8101 ccacccttgc gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta
     8161 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8221 cacggtttta tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt
     8281 cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     8341 tcaaccttgc gtaatgcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     8401 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     8461 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     8521 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     8581 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     8641 gctttgctct ccgtgttttc actcgcttac gggattgcgc aacttccttg cggcccacta
     8701 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     8761 caggcaatgt ctggcatggt gcacaacttt acgcagttcg tgttggtgcg tatcggtatg
     8821 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     8881 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc
     8941 gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt
     9001 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     9061 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     9121 cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg tacaatgtgg
     9181 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     9241 cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct
     9301 atccctttcc tgtttggggc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     9361 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc
     9421 tgttctgccg cctttacgct ggtcgtacca caagcgacaa catccatgac ggcggttctg
     9481 ctgattggca tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc
     9541 cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc
     9601 ttcatctgcg cctcttttgc gccgatcatt actggtttta ttgttgatac cacccactca
     9661 ttccgtctgg cactaatcat ctgcggttgc gtcaccgcag cgggggcact ggcgtacatc
     9721 ttcctggttc gtcagccgat caacgaccca cgtaaagatt aatctcctta tgcccgataa
     9781 cattttgctt atcgggcatt ttcatggcga ttaaaatgtt tcccagttct cctcgctatc
     9841 tgccacggcc attttacgcg gcgcagctgg cgtcacggtt tttaccacag ccgatgtttc
     9901 acgctgctgt tgctgttgtt gctgaatccg gaacactgcc acagcttcgg tcaggcgact
     9961 ggcctgctct tccagcgcgg cggcggcagc ggcagattct tccaccagcg cggcgttctg
    10021 ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg
    10081 ctcatcagaa gcagaagcaa tttcgcccat aatgtccgtc acgcgggtca cggcgctgac
    10141 aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc
    10201 caccgagtct tcaataaggc ttttaatttc acgagccgcc tgagcgctac gctgggccag
    10261 attacgcact tctcccgcaa ccaccgcaaa accgcgccct tgctcacccg cacgcgctgc
    10321 ttcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt caattacgct
    10381 gataatatcg gcgattttct gcgaactggt ggagatatcg cgcatagtct gcaccacgtt
    10441 atctaccact ttgccgcccc gttgcgccgt ttcagaagca cttaatgcca gatggctggc
    10501 ctggcgcgca ttctcggcgt tctgtttcac cgttgcggtc agttgctcca tgctggctgc
    10561 cgtctcttcc agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt
    10621 agcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt
    10681 acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga
    10741 gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgga tgctgtcaat
    10801 caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac
    10861 cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt
    10921 gttatcgctg acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg
    10981 cttctcgaaa ccgtcttgat atccctgggt cggctgatca aagaactcgt tgattttgcc
    11041 tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt
    11101 gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatccgc
    11161 ccagtttttt tccgcctgtt tcagcgaaat actggcactc tgcatcagct cagcaacggt
    11221 tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt
    11281 gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtggattgct gctggcgaat
    11341 ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaga acagaccgcc
    11401 tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt
    11461 gatacgtttt aacatggttt ctctttcctg tggaccgatg caagattttc ggcctggaaa
    11521 gcaaaaactt tatgaaaagg tgtaacccgc catccatgga aaaaccaggc gctggttatc
    11581 agatactaaa aagttattca ttatcgcccg ggatggcttc ttgtcgggaa ttgcccagag
    11641 caataaattc ctctaacaga tgggttaact gttgcatttt ctcggcagta aactgagctt
    11701 caatctgccg ataggcttct tcaatctgtg tctgagcgcg gttatacagc gcctgcccct
    11761 ctttggttag cgagatatag agcttccgct gatcattgat cggctttaat cgcaacacta
    11821 aaccgtcgcg ttccatccgc gtaaggatcc cggtcaggct tgggcgcaaa atgcaggcgc
    11881 gatacgccag atcgtgaaaa tccattgatg ggctttccgc cagaatacgc acgatgcgcc
    11941 actgttgctc agtcaaatta tgccgtttaa cgattgggcg aaaataactc atcgccgctt
    12001 ctcgcgcctg gagcaacgca atggttagag agtcgtgcat tatctttccc ctgaaaaatt
    12061 caaaatcatt aatatagaaa cagttaatca tcacgttacc gggactgcgc tgcccctgca
    12121 attcttcatt ttatcaaaat tgagcatgtt ttgttaactt gctgatattt aatattttat
    12181 tatctttaaa tgtaaatagt ttgttaatta gatcacattt acatcactta cttttgccaa
    12241 actacgcaat cgggaataaa agtaatcatt aacatattaa tgattaaaat gatcgaatcc
    12301 gaggagtggt aatgaaaggc actatcttcg ccgtagcgtt gaaccatcgc agccaacttg
    12361 atgcatggca ggaagcgttc cagcaatccc cctacaaagc cccgcctaaa actgcggtct
    12421 ggtttattaa accgcgcaat acggtgattg gttgcggtga accgattccc tttccacagg
    12481 gtgaaaaggt actgagcggt gcgactgttg cgctgattgt gggaaaaacg gcgacgaaag
    12541 tacgtgaaga agatgcggca gagtacatcg ccggatatgc gctggctaac gacgtcagcc
    12601 tgccggaaga gagcttttac cgcccggcaa tcaaagcaaa atgtcgtgat ggattctgcc
    12661 ccattggcga aaccgtggct ctcagcaatg tcgataatct gaccatctat accgagatca
    12721 acgggcgtcc tgccgatcac tggaataccg ccgatttaca acgtaacgcc gcgcagttgc
    12781 tgagcgccct gagcgaattt gccacactga atccaggcga tgccattctg ctcggcacgc
    12841 cacaggcgcg cgtggaaata cagccaggcg atcgcgttcg tgttctcgca gaaggtttcc
    12901 cgccgctgga aaatccggta gtggacgaac gtgaagtgac cacgcgcaag agcttcccaa
    12961 cgcagccaca cccgcacggc acgctgtttg ccctcggcct gaactacgcc gaccacgcca
    13021 gcgaactgga atttaagcca ccggaagaac cgctggtgtt cctgaaagcg ccaaataccc
    13081 tcactggcga taaccagacc tccgtgcgtc caaacaatat tgaatacatg cactatgaag
    13141 cggagctggt ggtagttatt ggcaaacagg cgcgtaacgt cagcgaagcc gatgccatgg
    13201 attatgtcgc gggctacacc gtgtgtaacg actacgccat tcgcgactat ctggaaaact
    13261 actaccgccc taacctgcgg gtcaaaagcc gcgacggact gacgccgatg ctttcaacca
    13321 tcgtgccgaa agaggcgatc ccggacccgc ataatctgac ccttcgcacc ttcgtcaacg
    13381 gcgagttacg ccagcaaggc accaccgccg atctgatctt cagcgtgccc ttcctgatcg
    13441 cctacttaag cgaatttatg accctgaatc cgggcgacat gatcgccacc ggcacaccaa
    13501 aaggcttatc tgacgtggtg cctggcgatg aagtggtggt ggaagtagaa ggcgtgggcc
    13561 gcctggtgaa ccgaattgtg agtgaggaaa cagcgaaatg aaaaaagtaa atcattggat
    13621 caacggtaaa aatgttgcag gtaacgacta cttccagact accaatccgg caacgggtga
    13681 agtgctggcg gatgtggcct ccggcggtga agcggagatc aatcaggcgg tagcggcagc
    13741 gaaagaggcg ttcccgaaat gggccaatct gccgatgaaa gagcgcgcgc gcctgatgcg
    13801 ccgtctgggc gatctgatcg accagaacgt gccggagatc gccgcgatgg aaaccgcgga
    13861 cacgggcctg ccgatccatc agaccaaaaa tgtgttgatc ccacgcgctt ctcacaactt
    13921 tgaatttttc gcggaagtct gccagcagat gaacggcaag acttatccgg tcgacgacaa
    13981 gatgctcaac tacacgctgg tgcagccggt aggcgtttgt gcactggtgt caccgtggaa
    14041 cgtgccgttt atgaccgcca cctggaaggt cgcgccgtgt ctggcgctgg gcaataccgc
    14101 ggtactgaaa atgtcggaac tctccccgct gaccgccgac cgcctgggtg agctggcgct
    14161 ggaagctggt attccggcgg gcgtgctgaa cgtggtacag ggctacggcg caaccgcagg
    14221 ggatgcgctg gtccgtcatc atgacgtgcg tgccgtgtcg ttcaccggcg gtacggcgac
    14281 cgggcgcaac atcatgaaaa acgccgggct gaaaaaatac tccatggaac tgggcggtaa
    14341 atcgccggtg ctgatttttg aagatgctga tattgaacgc gcgctggacg ccgccctgtt
    14401 caccatcttc tcgatcaacg gcgaacgctg caccgccggt tcgcgcatct ttattcagca
    14461 aagcatctac ccggaattcg ttaaacgctt tgccgaacgc gccaaccgtc tgcgcgtggg
    14521 cgatccgaac gatccgaata cccaggttgg cgcgcttatc agccagcagc actgggaaaa
    14581 agtctccggc tatatccgtc tcggcattga agaaggcgca accctgctgg cgggcggccc
    14641 ggataaaccg tccgacctgc ctgcgcacct gaaaggcggc aacttcctgc gcccaaccgt
    14701 gctggcagac gttgataacc gtatgcgcgt cgcccaggaa gagattttcg ggccggtcgc
    14761 ctgcctgctg ccgtttaaag acgaagctga aggcttacgc ctggcaaacg acgtggagta
    14821 cggcctcgcg tcgtacatct ggactcagga tgtcagcaaa gtgctgcgtc tggcgcgtgg
    14881 cattgaagct ggcatggtgt tcgtcaacac ccagaacgtg cgtgatttgc gccagccgtt
    14941 tggcggcgta aaagcctccg gcaccgggcg tgaaggcggt gagtacagct tcgaagtgtt
    15001 cgcggaaatg aagaacgtct gcatttccat gggcgaccat ccaattccga aatggggagt
    15061 ctgatatggg taagttagcg ttagccgcca agatcacgca cgtaccgtcg atgtatctct
    15121 ctgaactgcc agggaaaaac cacggttgcc gccagggcgc gatcgacggg cataaagaga
    15181 tcagcaagcg ttgccgggaa atgggcgtcg ataccattat cgttttcgat acccactggc
    15241 tggtcaacag tgcttatcac atcaactgtg cagaccattt tgaaggcatc tacaccagca
    15301 acgagctgcc gcattttatc cgcgatatga cctacaacta cgagggcaac ccggagttgg
    15361 ggcagcttat tgccgatgaa gccttaaagc tcggcgtgcg ggcaaaagcg cacaacattc
    15421 ccagcctgaa actggaatac ggcacgctgg taccgatgcg ctacatgaat gaagacaagc
    15481 acttcaaagt ggtctccatt tcggctttct gcacggttca cgattttgct gacagccgca
    15541 agctgggcga agcgattctg aaagcgatcg aacagtacga cggcaccgtg gcggtccttg
    15601 ccagcggttc gttatcgcac cgctttattg acgatcagcg tgcagaagaa gggatgaaca
    15661 gctacacccg cgagttcgac cgccagatgg acgagcgcgt ggtgaagttg tggcgcgaag
    15721 gccagttcaa agagttctgc aatatgctgc cggagtacgc cgactactgc tacggcgaag
    15781 gcaatatgca cgacacggtg atgctgctgg ggatgctcgg ctgggataaa tacgacggca
    15841 aggtggagtt tatcaccgag ctattcccaa gctctggcac cggtcaggtt aacgctgttt
    15901 tcccgcttcc cgcgtaagga accaccatgc cgcactttat cgtggaatgt actgaaaata
    15961 ttcgtgaaga agcccgctta cctgagctgt ttgccagtgt taacactgca ctggcggcaa
    16021 cggggatttt tccgcttggg ggaatacgta gccgggcgca ctggatcgat acctggcaaa
    16081 tggctgatgg caagcacgat tacgcctttg tgcatatgac gctaaaaatc ggcagcgggc
    16141 gcagcctgga aagccgtcag gaagtgggcg aaatgctgtt tgatctgatt aaaacgcact
    16201 tcgcctcact gatggagagc cgttacctgg cgctttcatt tgagattgca gagctgcacc
    16261 cgacgctgaa ttttaaacaa aacaacgtgc acgcgttgtt taaataaatt tgcaccagct
    16321 tccaggccgg atgataaggc gcagccgcat ccggcaatgc cacaggatat cgctatgttc
    16381 gataaacaca cccacaccct gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa
    16441 cagatccgcg cgatctcgct ggattacccg gagatcacca tcgaagacgc ttacgcggtg
    16501 cagcgtgaat gggttcgact gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc
    16561 ggcctgacct cgaaagcgat gcaggccagc tcacaaatca gcgagccgga ttacggtgcc
    16621 ctgctggatg acatgttctt ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg
    16681 ccgcgcattg aagtggagct ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc
    16741 acgctgttcg acgtctacaa cgccacggac tatgtgatcc cggcgctgga gctgatcgac
    16801 gctcgctgcc acaacatcga tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt
    16861 tctgataacg ccgccaacgc cggagtgatc ctcggcggtc gtccgatcaa accagacgaa
    16921 ctggatctgc gttggatctc cgccctgatg tatcgcaatg gcgtgattga agaaaccggc
    16981 gtcgccgctg gtgtgctgaa tcatccggca aacggcgtgg cctggctggc gaacaaactc
    17041 gcaccctatg acgtacaact ggaagccggg caaatcattc tcggtggttc gttcacccgc
    17101 ccggttccgg cgcgtaaggg cgacaccttc cacgtcgatt acggcaacat gggttccatt
    17161 agctgccgct ttgtttaagg agagaacgat ggaaaacagt tttaaagcgg cgctgaaagc
    17221 aggccgcccg cagatcggat tatggctggg gctgagcagc agctacagcg cagagttact
    17281 ggccggagca ggattcgact ggttattgat cgacggtgag cacgcgccga ataacgtgca
    17341 aaccgtgctc acccagctac aggcgattgc gccctacccc agccagccgg tggtacgtcc
    17401 gtcgtggaac gatccggtgc aaatcaaaca actgctggac gtcggcacac aaaccttgct
    17461 ggtgccgatg gtacaaaacg ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc
    17521 ccccgccggt attcgcggtg tgggcagtgc gctggcccga gcctcgcgct ggaatcgtat
    17581 tcctgattac ctgcaaaaag ccaacgctca aatgtgcgtg ctggtgcaga tcgaaacgcg
    17641 tgaggcaatg aagaacttac cgcagattct ggacgtggaa ggggtcgacg gcgtgtttat
    17701 cggcccggcg gatctgagcg ccgatatggg ttatgccggt aatccacagc acccggaagt
    17761 acaggccgcg attgagcagg cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat
    17821 cctgatcgcc aatgagcaac tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc
    17881 cgtcggcgtt gacaccaccc tgctcgcccg cgccgctgaa gcgctggcag cacggtttgg
    17941 cgcacaggcc acgaccgtta agcccggcgt gtattaatgc cggatgcgct gcacttatcc
    18001 ggcctacact cgcaccaaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa
    18061 gccgtaccaa atcgcggacc ggataaggcg tttgtgcgca tccgccataa accttgtcgt
    18121 accctacaaa aatcccatta gaggaagaaa aatgagcgac acctcacctg ccataccgga
    18181 gagtatcgat ccggcgaatc agcataaagc gctgactgcc ggacaacagg cggttattaa
    18241 gaagctgttt cgccgcctga tcgtctttct gttcgtgctg tttatcttct cgttccttga
    18301 tcgcatcaac atcggctttg ccggactcac gatgggacgc gacctcggtc tgagcgccac
    18361 catgtttggc ctcgctacca ccctgttcta tgccgcttat gtcatcttcg gcattcccag
    18421 caacattatg ctgagtattg tcggtgcgcg gcgctggatc gccaccatca tggtgctctg
    18481 gggcatcgcc tctactgcca ccatgtttgc cactggcccc accagcttgt acgtactgcg
    18541 tatactggtt ggcattaccg aagccggctt tctgcctggc attctgctgt atttaacctt
    18601 ctggtttccg gcctatttcc gcgcccgtgc caacgccttg tttatggttg caatgccggt
    18661 aacgacagcg ttgggatcgc tcgtttccgg ctacattttg tcgctggatg gcgtaatggc
    18721 attaaaaggc tggcagtggc tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt
    18781 catggtgtgg ttctggcttg atgactcacc ggacaaagct aagtggctga cgaaagaaga
    18841 caaaaaatgc ctgcaagaga tgatggataa cgatcgtctg acgctggttc agccagaggg
    18901 agccatcagc caccacgcca tgcaacaacg cagtatgtgg cgggagatct tcactccggt
    18961 ggtgatgatg tataccctgg cgtatttctg cctgaccaac acacttagtg cgatcagcat
    19021 ctggacaccg cagatcctgc aaagttttaa tcagggcagc agtaatatca ccatcggcct
    19081 gctggccgcc gtaccgcaga tttgtaccat tctcgggatg gtctactgga gccgtcactc
    19141 agatcgccgc caggaacgaa ggcatcacac cgcccttcct tatttgttcg ctgccgcagg
    19201 ttggttactg gcttcggcaa ctgatcacaa catgatccag atgctgggga tcattatggc
    19261 ttcgaccgga tcattcagcg caatggcgat tttctggaca acaccggatc agtccatcag
    19321 cctgcgggca cgagcgatcg gtattgcggt gatcaacgcc actggcaaca ttggttcagc
    19381 gttaagtccg tttatgatcg gctggttgaa agatctgacc ggcagcttta acagtggatt
    19441 gtggtttgtt gccgcgctgc tggtgattgg tgcggggatt atctgggcga ttccaatgca
    19501 gtcctcccgt ccgcgagcga ccccgtaagg aacgacgatg tgtgaccgtc agattgccaa
    19561 tattgatatc agcaaagagt acgatgaaag cctgggcacg gacgatgtgc attatcagtc
    19621 gttcgcccgc atggcggcct tttttggccg ccatatgctg ccacatcgcc acgaacagta
    19681 ctttcagatg catttcctca atagcggaca gattgagcta cagcttgacg atcatcgcta
    19741 ctcggtggaa gcgcccctgt ttgtcctgac gccaccgtca gtacctcatg cgtttattac
    19801 ggagtctgat gctgacggtc atgtattgac ggtacgggaa gatctgatct ggcccctgct
    19861 ggaagttctt tatccgggca ctcgggaaac cttcggcctg ccggggattt gcctgtcact
    19921 ggcagataaa cccgacgaac tggcggcgct ggaacactat tggcaactga tagagcggga
    19981 atcggtagaa caactgcctg gacgggaaca caccctgacg ttactggcac aggcagtgtt
    20041 caccctactg ctgcgtaacg caaaactcga cgaccatgcc gccagcggaa tgcgcggaga
    20101 attaaaactg ttccagcgtt ttcatatgct tattgaaagc cattttcatc agcactggac
    20161 agtaccggat tacgctaacg aactgcatat caccgaatca cgcctcacgg acatctgccg
    20221 ccgctttgcc aaccgtccgc caaaacggtt gattttcgac aggcagctac gagaagccaa
    20281 gcggctgctg ctgttttctg ataacgccgt gaacaatatt gcctggcaac tcggttttaa
    20341 ggatccggct tattttgcgc gcttttttaa tcgcttagtc ggttgctcgc ccagtgctta
    20401 tcgtgccaaa aaagtacctg tgacgtgaaa ttcccttagc cttaacggga aaccaggcac
    20461 cacctgctat tcccctttct tcgcctggag caaggggatt tagtaacgtt aaaaatcgct
    20521 aaaagcgacc tcgatcacaa aaccgtaccc gacctgaaaa gtaccagcca ttgtcccaaa
    20581 agtgtcttcc cgtaatcgcc cctgcaagct tcaattgaat aacaaaaaca acacacaaaa
    20641 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    20701 ccagtaccca acgtccgttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    20761 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    20821 cggcagcgtc tgttgcccaa ctgtacgacg cgctacacaa accggagatg caggactctc
    20881 tgtgctggaa caccgacacc ggtagcggcg gctataccca taaattcttc cgcgtggcga
    20941 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgagtggtca cgcctgagct
    21001 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    21061 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    21121 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgatcgt catttgccga
    21181 ccgataaagt gaaagacgtt tacatcaagc tggaaaaaga gactgacgcc gggattatcg
    21241 tcagcggtgc gaaagtggtt gccaccaact cggcgctgac tcactacaac atgattggct
    21301 tcggctcggc acaagtgatg ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa
    21361 tggatgccga tggcgtgaaa ttaatctccc gcgcctctta tgagatggtc gcgggtgcta
    21421 ccggctcgcc gtacgactac ccgctctcca gccgctttga tgagaatgat gcgattctgg
    21481 tgatggataa cgtgctgatc ccatgggaaa acgtactgat ctaccgcgat ttcgatcgct
    21541 gccgtcgctg gacaatggaa ggcggttttg cccgtatgta tccgctgcaa gcctgtgtgc
    21601 gcctggcagt gaaactcgac ttcattacag cactgctgaa aaaatcactc gaatgtaccg
    21661 gcaccctgga gttccgtggt gtacaggccg atctcggtga agtggtggcg tggcgcaaca
    21721 ccttctgggc attgagtgac tcgatgtgtt ctgaagcgac gccgtgggtc aacggggctt
    21781 atttaccgga tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga
    21841 agatcaaaaa cattatcgaa cgcaacgtta ccagtggcct gatctatctc ccttccagtg
    21901 cccgtgacct gaacaatccg cagatcgacc agtatctggc gaagtatgtg cgcggttcga
    21961 atggtatgga tcacgtccag cgcatcaaga tcctcaaact gatgtgggac gccattggca
    22021 gcgagtttgg tggtcgccac gaactgtatg aaatcaacta ctccggtagc caggatgaga
    22081 ttcgcctaca gtgtctgcgc caggcacaaa gctccggcaa tatggacaag atgatggcga
    22141 tggttgatcg ctgcctgtcg gaatacgacc agaacggctg gactgtgccg cacttgcaca
    22201 acaacgacga tatcaacatg ctggataagc tgctgaaata acgcagcagg aggttaagat
    22261 gcaattagat gaacagcgcc tgcgctttcg tgacgcgatg gccagcctgt cggcagcggt
    22321 aaatattatc accaccgagg gcgacgccgg acaatgcggg attacggcaa cggccgtctg
    22381 ctcggtcacg gatacaccac catcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa
    22441 cccggttttt cagggcaacg gcaatttgtg cgtcaacgtc ctcaaccatg agcaggaact
    22501 gatggcacgc cacttcgcgg gcatgacggg catggcgatg gaagagcgtt ttagcctctc
    22561 atgctggcaa aaaggtccgc tggcgcagcc ggtgctaaaa ggttcgctgg ccagtcttga
    22621 aggtgagatc cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg tggagattaa
    22681 aaacatcatc ctcagtgcag aaggtcacgg gcttatctac tttaaacgcc gtttccatcc
    22741 ggtgatgctg gaaatggaag ctgcgattta agctccagcc ccttcacgta ttcgtcgaag
    22801 gggcattttt attgtgatct caatcacata tccacccgat gaataattct tgcccaaccg
    22861 ctcaccgcca ttcctgaccg cttacgtccc taaaataacc actcagttat ttaccttact
    22921 ttacgcgcgc gtaactctgg caacatcact gcaggatagc ggtcaattta cctcctcaaa
    22981 cgcaacgcaa acctagaacg gcttcggcct atgattaaca aattactcta tgaattttgg
    23041 tttgcaggaa ggcagcaagt gagtgaatcc ccgggagctt acaacagtaa gtgacagggg
    23101 tgaacgaacg caactgccgc acctgtaagc caaaagacga cgagtaaaat gccaggtttt
    23161 actatggata cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt
    23221 gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc
    23281 gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtacatcgcc
    23341 cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt
    23401 ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc
    23461 ggtgctggtc cgctggttgg tccggttctc gccgcgcaga tgggctacct acctggcacg
    23521 ctgtggctgc tggcgggggt agtactggcc ggtgcggtac aggactttat ggtgctgttt
    23581 atctcctctc gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatgggacca
    23641 gtaccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat ccttgccgtc
    23701 ctggcgctga ttgtggttaa agccctggcc gaaagcccgt ggggtgtttt caccgtttgc
    23761 tcaaccgtac cgattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt
    23821 gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta cttcggtggc
    23881 gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc
    23941 ttcgcgctga ttggctatgc gtttgtttcc gcactgctgc cggtgtggct gatcctcgca
    24001 ccgcgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgctgggt
    24061 attgtggtgc tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact
    24121 ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg
    24181 gtatctggct tccacgcgct gatctcttcc ggtacgacgc cgaaactgct ggctaacgaa
    24241 accgacgcgc gtttcattgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg
    24301 gcgctggttg ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct
    24361 gctggccttg gcattaccat gcctaacctg catgaaatgg gtggcgagaa tgcgccgatc
    24421 atcatggcgc agctgaaaga cgttaccgca cacgcggcgg caaccgtcag ctcctggggc
    24481 ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc
    24541 ctgaaccgtg caggtggtgc gccaacgctg gcggtaggta tcgctcacgt gttccacaaa
    24601 gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg
    24661 ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc gttttatgct gcaagacctg
    24721 ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgccgg tatcatcggt
    24781 actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg
    24841 ggcggcgtta agagcctgtg gccgctgttc ggtatttcca accagatgct ggcagccgta
    24901 gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta
    24961 actgttgttc cggctgtatg gctgcttatc tgcaccacct gggcgctggg tctgaaactg
    25021 ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag
    25081 attgctaacg gtactgacct gacggcgcag cagattgcca acatgaacca catcgttgtg
    25141 aacaactaca ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc
    25201 ttctacggtt tcaaaacctg gcttgcggta cgtaacagcg acaaacgtac tgacaaagaa
    25261 actccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccgtgt
    25321 ttagccccgc ttcggcgggg ctttgttctt atcagagtga actatgtttg gtaacttagg
    25381 acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc cagactatga
    25441 caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga
    25501 attcttccgc gaacgccaga acgcacgcta cggcggcgat ggtaaaggcg gtatgcgctg
    25561 ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg
    25621 cgggcaaaac caccctgctg cgccatattc ttaatgaaca acacggctac aagattgccg
    25681 tgattgaaaa cgaattcggc gaagtctccg ttgatgatca attgattggt gatcgcgcaa
    25741 cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggagg
    25801 acgcgctact cgacctgctg gataatctcg acaagggcaa tattcagttc gatcgtctgg
    25861 tcattgaatg caccggcatg gccgatcctg gcccgattat tcaaaccttt ttctcccatg
    25921 agattttatg ccagcgctac ctgctggacg gcgtgattgc gctggtggat gcggtacatg
    25981 ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggtgggctac gccgaccgta
    26041 ttctgctgac taaaaccgac gtcgcaggcg aagcagaaaa actgcgtgaa cgtctggcac
    26101 gcatcaacgc ccgtgcaccg gtctacaccg tcacccacgg cgacatcgac ctgggtctgc
    26161 tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa ccgcgtttcc
    26221 attttatcgc ggacaaacaa aacgatattt cgtcgattgt ggtggaactg gattacccgg
    26281 tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg gcggataaac
    26341 tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg ctgttccagg
    26401 gcgtccagcg cctctacagc gccgactggg acaggccatg gggcgatgaa acaccgcata
    26461 gcacgatggt gtttatcggg attcagttgc cggaggatga gattcgggcg gcgtttgcgg
    26521 gactcaaaaa gtaaccagcc ctccaacatc acaatacctg agactttatc accctcatat
    26581 tcctccagaa tatgagggct ttcgggctta attatctctg aacgtgcaac acttcctgcg
    26641 actccttatg cgcctccaca tcctgcttcg ttaaccagag cgacttacgg ccaaaatttt
    26701 cgtacatttt cagctgatct tcatagtgct tatcaactgt tccatcggga gcaataaacc
    26761 cactctgacc gggtgcgacc acatcccagg caagcacagg acgatcgctt gtcgttggtg
    26821 agaaaacaat catatcgttt tctgttccac ggttttgata ctccgcctga tgacgcgttt
    26881 cttccgctgc ggcctgcggt acaccaaaga aattatttgc ccggaacgtt aaggccattg
    26941 caggtgtttt ccagttactc acattattgc catagcgttt ggaaagagtc tcccaggtat
    27001 cttccagcgc agccaacaca acctcctgct gtggtttccc agcaaacaga tcaaccgcct
    27061 gtgggattgg tgatttgtct ccctgcaccg cctcatacaa aatttttgct ccaacactta
    27121 tattcagcga accagttggg ccgtcctggg ttgtttcgta gccactggcg ctgtaccact
    27181 tatcaaatgg cataggtacg gcagccacta cggtacgctt caacatactg gtcagccaaa
    27241 cgttcaggat ggcagagcct ggctgctgcc aggttttacc atcatcatta agcaaattga
    27301 tgccatccca acgtgttaat gtttctacca actgacgacg cggatcgctc tgtgtcaaac
    27361 cagatgtcgc tgcttgcaga gtaggtaaaa aaagcctcag gttaagatcc tgacgactgg
    27421 tttggcgaat aacatcccat gcctgatcag cagttaagcg tggcttttgc tcaagcagtc
    27481 ggtcgatctc cgtaacgcga tctgcaccac cccacaaaaa ggcaaacaga tctgaagcgg
    27541 gataatcttt ttggggagaa ttgttccagt tagcaatata tcccgactgg gggttataca
    27601 ccttagggtt catttcaaaa ggcaatagcc ctttccagtc ccattttccc gtaccaggaa
    27661 cgggtaatcg cggatcatgg cctgattgac gatctggata agcaccagta tgaacataac
    27721 caatattgcc gtttacatca gcatagtacc agttgatggt cagtgcttgt ttcgctgcct
    27781 gctgtgtcca ctcctgccaa tttttggcct tcatctgatg agtccaggcc agcaaagacg
    27841 ccacctcttt accatcccat gcgcgggatt tagcgtaagc cgtttgtgtc gtctggtcag
    27901 tttggagaat gttgccatgc accgtacgcc agacagtaaa ggtctctgcc tgaccatttt
    27961 tcaccgtaat ggtttcctca cggcttaaca ttttcaccca cttaccatta tgcaagtagt
    28021 agcctggttt ctctgccgac agccgttcag caaaaatatc gacatcatcg ccgaaacctg
    28081 ccgttgatcc ccaggaaatc acaccattat gaccaaaaac cagcccagga taggcaaatg
    28141 gtgtattgcc agtgacatca taaccagcac cgtgcagacc aataccataa gtatacgcag
    28201 gcgcatacca gccaaactgc ggaccattta ccatgattgc tttcgcatcc tgggctttgc
    28261 ttttgccgat cacccacata ttgctggtcg ttggataccc cgccagacca ttggcaccac
    28321 cctgtgcaaa ttgtgcagca atagtttccc ggttcttccc tgctgttaac gccagcagtg
    28381 cgccatccgc cccttttgct ggtcggtcaa gcattggtgc aggtaaatcg tagcgtggca
    28441 acagagctgc tgtttgcgag ttttgctgat taaatttaag tgggtagtta ctctcttgta
    28501 cggcaatagt ggttggcgct gatgggttta ccagccattt caactgatta aataccgcca
    28561 tgccttgtga tacaccatat ttatctttta aagccgttag cagtgccaaa ttatcaattt
    28621 cgctagtgct atcagagaag cggtttgcca tggtgcccac aaatatcatc gcgacatcaa
    28681 acggttccca gcgcttagga gtaaagccaa atgtattaaa ctgttttggt aagagcgtct
    28741 ctggattggt atttacctta tcaatccagg cattcattcc atcagcgtag ccttgcagaa
    28801 tggacatatc ctctggggaa agggcagcaa tttgcgcccg gatagcatcc ggccagtagt
    28861 tacgacggat atctttatca aatttcacaa aatctttgcc aagcacttcc gcgacagtcc
    28921 cttgagtact gcgacgtgcc atttccatct gaaaaaggcg atcttgtgct actacatagc
    28981 catagccata aaataggtgc catgtatcat tggcataaat atgcggcatg ccgtattcat
    29041 cgcgaacaat ctttatctca cttgacgact gctcagccag tgcaggtaag ctccaataat
    29101 acatcaggga agcagtaaca cagttcacga tcatacgatt tctatttttc attgtatcct
    29161 ctggcaggtg tataattagc gaattgatac tagcaacgaa gcttcggtta tctatttgca
    29221 gagaattgtt tcattatgaa ctgtgagcgc gataactaat ttttgataca aaaatttcat
    29281 taatgtgatc taaaacgaat cgctttattc accgctgatg attttgttct attccttttt
    29341 gtgacagatc ccccggacgc atagatgatg acccccagag agacagctca ggtcattatc
    29401 tgtatgtaaa tcagaaaaat atgttgtaaa cgacttttat ggttggattt tgtagcaatt
    29461 ttgaatgcag acaggcagca tttgccaaca cgccgcccat agttcactcc acatcctccc
    29521 tgcgtaaccc aatatccctc aaccgttcat cactcatctg ctgtaacact cgtcgggtct
    29581 gcatttgccg ccgccaacgc ctcaccgcat gccagagttg cacaagtctg ataaacggcg
    29641 ttttggcttt gttttcgtga aattccatct gctgcctcct catctttcag aggcttcatc
    29701 atccgccttc tggcagttca caatacagat gcaaatatta gttttattta acatacagac
    29761 aggttaaaac agcatctgca tccggttttt tagcctcatc tgtaccggtt tttctttctg
    29821 tatgggccaa ataaggaata cagcatgacg cgttatcaac atctggcgac tctacttgcc
    29881 gagcggattg agcaagggct gtatcgtcac ggggagaaat tgccgtcggt gcgcagctta
    29941 agtcaggagc acggcgtcag catcagcacc gtgcaacagg cgtatcagac gctggaaacg
    30001 atgaggctca tcactccgca gccgcgttcg ggttattttg tcgcacaacg taaagcccag
    30061 ccgcctgtac cgccgatgac gcgtccggtg cagcgcccgg tggaaattac ccagtgggat
    30121 caggtgctgg atatgctgga ggcgcatagc gacaattcca ttgttccgtt aagcaaaagc
    30181 acgccggatg tcgaaacgcc cagcctgaaa ccgctgtggc gtgagctaag ccgggtggtg
    30241 cagcataatc tgcaaaccgt tctcggttat gacttgttag ccggtcagcg agtgttgcgg
    30301 gagcagattg cccgcctgat gctcgacagc ggctcggtgg tcaccgccga tgacatcatc
    30361 atcaccagcg gctgccataa ctcgatgtcg ctggcgttaa tggcggtgtg taaaccgggc
    30421 gatattgtcg cggtcgaatc tccctgttat tacggttcga tgcagatgct gcgaggcatg
    30481 ggcgtgaaag tgattgaaat cccaaccgat ccagaaactg gcatcagcgt tgaagcactg
    30541 gaactggcgc tggaacagtg gccgattaaa ggcatcattc tggtgccaaa ctgtaataat
    30601 ccgctgggat ttattatgcc ggacgcacgc aaacgggccg ttctctctct cgctcagcgt
    30661 catgatattg tgatttttga agatgatgtc tacggcgaac tggcaacgga gtatccgcgc
    30721 ccgcggacca tccattcatg ggatatcgac gggcgagtgt tgttgtgcag ctcgttcagt
    30781 aaaagtattg ctccaggcct gcgcgtgggt tgggtcgcac cggggcgtta tcacgataaa
    30841 ctgatgcata tgaaatacgc catcagcagc tttaatgtgc cgtccacgca aatggcggcg
    30901 gcaacgtttg tgctggaagg tcactatcat cgccatatcc ggcggatgcg gcagagctat
    30961 cagcgcaatt tggcgcttta tacctgctgg atacgggaat attttccctg cgaaatctgt
    31021 attacgcgcc cgaaaggcgg atttttactg tggatagaat tgcctgaaca ggtcgatatg
    31081 gtctgcgttg cgcggcagct gtgccgcatg aatatccagg tggcagcagg ctcgattttc
    31141 tcggcttccg gcaaataccg taattgtcta cgcatcaact gcgctttgcc gctcagcgaa
    31201 acctatcgcg aagcactaaa gcaaattggc gaggccgtgt atcgggcaat ggaataaatt
    31261 cgaattagtg gcgaagcacc tgatttcctc tcacgaatcg cctgtaactc gttgtatctt
    31321 tgcagggtca tcgccacgtt ccagcatggc ttaggcaaag tgtgcttcgt tcataccgga
    31381 tgcggcgtga acgccttatc cggcctacaa accgcgcaaa ttcaatagat tgcagaggaa
    31441 atgtaggtct gataagcgta gcgcatcagg cagctttgcg tttgtcatcc gtctctgatt
    31501 agtattcgat ccatggatcg cgcttgattt caggcaaggg ctgctctaat gcctgcattt
    31561 gctctgtcga aagtccggta atcttttgta tccattcagg atctgcccca ttctgcaaca
    31621 acaatctaag gaggcgttgt tccccttctt ctttcccctt ttccatcccc aacagccatc
    31681 catcattata aattcgctct gcaatcgtca ttattcgctc cctgtgttgt ggcattcgac
    31741 gggtaagttc gctgataaac ttcttaaaac gcgcttcatc gccagtcagt aaaatgtaat
    31801 ttaacagcgc agttatctgg ctgtcattag cacactctgt aaccagtaat attaccagtt
    31861 gatcgataag ccccatcaga tcgcgctggc gaatatgctt ttggatcaac tccaacaggg
    31921 cgactctgcg gtgctgcaca atctcgtcgt ctggcacgac agtgacatcc accagcggga
    31981 acgctgcgct ataaagcttc cgtgcggtgg tcgggtcagc aaattcgtcc agccagcaca
    32041 gggaccaggg ataaggacta cggctaccgt gataaaacag catcgggatg accagcggta
    32101 gcggccggcg tttatcatgc tcgatatggc gctgcatcac cgccatggaa tatcgcatca
    32161 ggcgaaaggc catatggata tcctcgcggc tctgatgttc aatcactacg taaatataac
    32221 catcaccttc acgggtcttt accgaccaca gaatatcgga gtgtagcgcc cgcaattttt
    32281 catcgacgaa gctggcggat tccagtttta agctgtcgag atcgcacagt tcacgtaaat
    32341 ctttgggtaa gtggatctcc ataaaatccc gcgcggtgtc agggtgcgtg agaaaggatt
    32401 taaataatgc gtcatgcggc gtgctggttg tgaagtttgt catggcgatc cgtcacctgt
    32461 agataagatg gcccgactgt agcgactaac aaaaccattc tcacccggca atttctcgtt
    32521 ccccgaggcg ctttccaggc tatttgtgtt cctctgcaaa cgatacattt ttccggaaac
    32581 aggctcgcgt tttacatttc ctgtacccgg cagatattcc cctttccacc gcgctctttt
    32641 gattaaccgc aaattttaat tacacttaag gtgtatattt tctatgcaac ccatcaatta
    32701 aagaggtgta atgtgctgat gactatttgt aatcgttata cattctgacc tggagtcaga
    32761 aggtatttct ctgtctgtgt gttcacaggc agtgtggttg attacatgaa attcagtaca
    32821 tttgtccttc tcgcctgcct ttcgtcatta ccgacggtat tgaatttcgt tttccccgtt
    32881 ggggttctcc ggacaaggag ttgtttgtta tgccacgttt ttttgcccgc catgccgcta
    32941 cgctgttttt cccgatggcg ttgattttgt atgactttgc cgcctatctt tcgacggatc
    33001 tgatccagcc ggggatcatt aatgtggtcc gtgattttaa tgccgatgtc agtctggctc
    33061 cggctgccgt cagtctctat ctcgcggggg gtatggcgtt acagtggctg ctggggccgc
    33121 tttccgacag gattggccgc aggccggtgc tgattaccgg ggcgctaata tttacccttg
    33181 cctgcgctgc gacaatgttc acaacgtcta tgacacagtt tcttatcgcg cgtgcaattc
    33241 agggcaccag tatctgtttt attgctaccg ttggttatgt cacggtgcag gaggcgttcg
    33301 gacagacaaa agggattaag ttgatggcga ttatcacctc catcgtactg attgcgccga
    33361 ttattggccc gctttccggc gcagctctga tgcactttgt gcactggaaa gtcctttttg
    33421 ccatcattgc ggttatgggg tttatctcat ttgttggctt actgctggcg atgccagaga
    33481 cggtgaagcg cggcgcggtt ccatttagcg ccaaaagcgt cttgcgcgat tttcgtaatg
    33541 tcttttgcaa ccggctgttc ctctttggcg cagcaaccat ctctttaagc tatatcccga
    33601 tgatgagttg ggtggctgtc tcgccggtga tcctgattga cgcaggcggc ttaacaactt
    33661 cgcagttcgc ctggacacag gttccggtgt tcggcgcggt gattgtcgcg aatgccatcg
    33721 tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat ctggcgtgct gtacccattc
    33781 aactggtcgg cctcgcgctg ttgattatcg gcaatctgct gtcaccgcac gtctggctgt
    33841 ggtcggtgct gggcaccagt ttgtatgctt tcgggattgg tttgatcttc ccgaccttat
    33901 tccgctttac gcttttttcc aataacttac cgaaaggaac cgtctccgca tcgctgaata
    33961 tggtgatcct gatggtgatg tctgtctcgg tcgaaatcgg ccgctggctg tggtttaacg
    34021 gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg cgttatcgtc gttttcaccc
    34081 tggcaggatt gctcaatcgc gtgcgccaac atcaggctgc cgagctggcg gaggagcggt
    34141 gatttttgcg cgatcctgcc gtcaggctct attcttaacg ttatgaataa actcattgaa
    34201 ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta tcgccgctgc tacctttgtc
    34261 gttacgctgt ttttgccgcc caatttttgg gtgagcggcg tgaaggcgat tgctgaagcg
    34321 gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg cgctgtttcg ccgcgtgccg
    34381 attccgatca tttctcgtca tacggcgatt atcccgcgta ataaagaccg gattggcgaa
    34441 aatctcggcc agttcgtgca ggaaaaattt ctcgataccc agtcgctggt ggcattgatt
    34501 cgacgtcacg aaccggcgtt gttgattggc aactggttta gtcagccgga gaacgcccgc
    34561 cgcgttggtc agcatctgtt gcagatcatg agcggttttc ttgaactgac cgatgatgcg
    34621 cgtattcagc gcctgcttaa gcgcgcggtc catcgggcga ttgataaggt cgatctttcc
    34681 ggcaccagtg cgttgatgct ggagagtatg accaaaaacg atcgtcatca ggtgctgctg
    34741 gatacgctga tcgcgcagtt gattgctctt ctccagcgcg ataaatcgcg caagtttatt
    34801 gcccagcaaa ttgttcgctg gctggagagc gagcatccgc tgaaagccaa aattttgcct
    34861 actgaatggc tgggcgaaca tagcgcggag ttggtttctg acgcggtgaa ttctttgctt
    34921 gatgatatca gccgtgatcg tgcgcatcag atccgttatg cgtttgatcg cgccactttt
    34981 gctctgatcg acaagttgaa aaacgatccg gaaatggcag cgcgagccga tgccgtaaaa
    35041 agttatctga aagaagatga agcttttaac cgctatctca gtgaattgtg gggggattta
    35101 cgggagtggc tgaaagcgga tatcaacagt gaagattctc gtgtgaaaga acgtatcgcg
    35161 cgggcgggtc aatggtttgg cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta
    35221 aatggtcacc tggaacaagc cgcgcaccgc gtcgcgcctg agttttccgc attcctgacg
    35281 cgccacatca gcgatacagt aaaaagctgg gatgcgcggg atatgtcacg gcaaatcgag
    35341 ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg gtacgctggt tggcggttgt
    35401 attgggctaa ttttatattt gttgtcgcag ctcccggcct tgttccccct cggcaatttt
    35461 tagaaatcca tgaaaaacta agtggctaac cgcgagagag atcaaataaa ccacactaaa
    35521 agaatggtaa taatgtggcc tcttttcatt atctcccgtg gtacggggaa ggaaaatcat
    35581 gtcacttatc accgatctac ccgccatttt cgatcagttc tctgaagctc gccagaaagg
    35641 ctttctcacc gtcatggatc tcaaggagcg cggcattccg ctggttggca cttactgcac
    35701 ctttatgccg caagagatcc cgatggcagc cggtgcggtt gtggtttcgc tctgttccac
    35761 ctctgatgaa accattgaag aagcggagaa agatctgccg cgcaacctct gcccgctgat
    35821 taaaagtagc tacggcttcg gcaaaaccga taaatgcccc tacttctact tttcggatct
    35881 ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg tatgaataca tggcggagtt
    35941 taagcctgtt catgtgatgc agctgccgaa cagcgttaag gacgatgcct cgcgtgcatt
    36001 atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat
    36061 tagcgaagat gctctgcgcg atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc
    36121 taatttttat catcttgggc agttaaatcc tccggcgctt agcggcagcg acattctgaa
    36181 agtggtttac ggcgcaacct tccggttcga taaagaggcg ttgatcaatg aactggacgc
    36241 gatgaccgcc cgcgttcgtc agcagtggga agaaggccag cgacttgacc cgcgtccacg
    36301 cattttaatc accggctgcc cgattggcgg cgcagcagaa aaagtggtgc gggcgattga
    36361 agagaatggc ggctgggttg tcggttatga aaactgcacc ggggcgaaag cgaccgagca
    36421 atgcgtggca gaaacgggcg atgtctacga cgcgctggcg gataaatatc tggcgattgg
    36481 ctgctcctgt gtttcgccga acgatcagcg cctgcaaatg ctcagccaga tggtggaaga
    36541 atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg tgccatacct acgcggtgga
    36601 atcgctggcg attaaacgtc atgtgcgtca gcagcacaac attccttata tcgctattga
    36661 aacagactac tccacctcag atgtcgggca gctcagtacc cgtgtcgcgg cctttattga
    36721 gatgctgtaa ggagtggcag tgacatattc gattggcatt gattctggtt ctaccgccac
    36781 caaagggatc ttactggcag acggcgtgat tacgcgccgt ttcctcgttc caacaccctt
    36841 tcgtccggca acagcaatta ctgaagcctg ggaaactctg cgcgaagggt tagagacaac
    36901 gccgtttctg acgctcaccg gctatggacg gcaactggtg gattttgccg ataaacaagt
    36961 gacggaaatc tcctgtcacg ggctgggcgc acggtttctt gcgccagcaa cgcgcgcggt
    37021 aatcgacatc ggtggtcagg acagcaaagt gattcagctt gatgatgacg gtaacctgtg
    37081 cgatttcctg atgaatgaca aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc
    37141 gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt accgaaaatg tcacgccgca
    37201 tgccatcacg agtatgtgca cagtgtttgc cgaatcagaa gtgatcagcc tgcgctcagc
    37261 gggcgtcgcg ccagaagcga ttctcgccgg agtgattaac gcgatggcgc ggaggagtgc
    37321 caatttcatt gctcgtctct cctgtgaagc gccgattctg tttactggtg gcgttagtca
    37381 ttgccagacg tttgcccgga tgctggaatc tcacctggga atgccggtaa atacccaccc
    37441 tgatgcgcaa tttgctggcg caattggcgc ggcggtaatt ggtcaacgag tgaggacacg
    37501 ccgatgaaag agtttttatt tttatttcac tccacagtcg gcgtcataca aacccgcaaa
    37561 gcgttgcagg cagcgggcat gacttttcgc gtcagcgata ttccccgtga tttacgcggc
    37621 ggctgcgggt tatgcatttg gctgacctgt ccccccggcg aggaaataca atgggtgatc
    37681 cctgggctga ctgagtcaat ttattgtcag caggatggtg tctggcgctg cattgcacat
    37741 taccgggttt ctccgcgtta aggattcatt aaggtcgcta cgtatacttc tcgatatctt
    37801 attgatttgc cttaaaggga ttaccgtgac aaaaagtatt tcgttaagta aacgcatctc
    37861 cgttattgtg attctttttg ccattgtggc agtgtgtact ttcttcgtgc aatcttgcgc
    37921 ccgtaaaagc aatcatgcag catcatttca gaattaccac gccaccattg acggcaaaga
    37981 aattgcaggc ataacgaata atatctcttc gctgacctgg tcggcacaaa gcaatactct
    38041 ttttagcact atcaacaaac ctgccgccat tgttgaaatg accaccaatg gtgattttat
    38101 ccgcacaatt cctctggatt ttgttaagga tctcgagacg atcgaataca ttggcgataa
    38161 tcagtttgtc attagcgatg agcgcgatta cgccatttat gtgatttcac tgaccccaaa
    38221 ctcggaagta aaaatcctca aaaaaataaa aatcccgctg caagactccc caaccaattg
    38281 cggttttgaa gggttggctt attctcgcca ggatcacact ttctggtttt ttaaagagaa
    38341 aaatccgatt gaggtctaca aagttaacgg gctgttaagt agcaatgaat tacacatcag
    38401 caaagacgag gcactacaac ggcaatttac tttagatgat gtgtccggcg cagaatttaa
    38461 ccagcaaaaa atacgcttct ggtgctgtct catgaatctc gcgcactcca ggaagtaacc
    38521 ctggttggcg aggttatcgg tgaaatatca ctaaccaaag gtagtcgagg tttgtcgcat
    38581 aacatcaaac aggccgaagg gattgcgatg gatgcctctg gtaatattta tatcgttagc
    38641 gaaccaaatc gtttttatcg ctttacccct aaatcatcgc attaacaggc tggcatattt
    38701 cacctttttg ttattaactt gttaacaaca aaaatgacat acaaggttaa acatgccttc
    38761 gtcctcgcat cccgtagaac gcttttcttt cagcaccgcg cttttcggga tgctggttct
    38821 gaccctaggt atgggattag gtcgcttcct ttatacgcct atgttgcccg tcatgatggc
    38881 ggaaggggcg ttttcattta gccagctctc gtggattgcc agcggcaact atgcggggta
    38941 tctggctggc agtctgctat tttcgtttgg cgcatttcac cagccgtcgc gcctacgccc
    39001 atttctgttg gtttctgccc tggcgagcgg gttgttgatc ctcgcaatgg catggttgcc
    39061 gccatttatt ctggtgttat tgattcgctt cctggcgggt gtcgccagcg ccggtatgct
    39121 gatttttggt tcgacgctga ttatgcagca cacgcgccat ccttttgtgc tggcagcttt
    39181 gttttctggc gttggcattg gcattgcact gggcaacgaa tatgttctgg caggcctgca
    39241 ttttgcctta tcctcgcaaa cgttatggca aggcgccggg gcgctttctg gcatgatgct
    39301 gattgcactt acgcttttaa tgccctcgaa aaaacacgcc atcgcaccaa tgccattggc
    39361 aaaaacggag caacagataa tgagctggtg gttactggct attctgtacg gccttgcggg
    39421 atttggttat atcatcgtcg cgacttacct gccgctcatg gcgaaagatg caggctcacc
    39481 attgttaacc gcccatctct ggacgttagt cggcttatca attgtgccag gctgctttgg
    39541 ctggctatgg gcagcaaaac gttggggggc tttgccttgc ctgacggcga atttgctggt
    39601 gcaggctatc agtgtgctgc ttactctcgc cagcgactct cctctcttgc ttatcattag
    39661 tagtatcggt tttggcggta cctttatggg cacgacatct ctggtgatga ctatcgcccg
    39721 ccaactgagc gttccgggaa atctcaatct tttaggcttt gtgacgctca tttacggcat
    39781 tggacaaatc ctcggcccgg cgctgaccag tatgctcggc aatggaacat cggcgcttgc
    39841 cggcgccact ctctgcggcg cggcggcgtt gtttattgct gcgcttatca gcacagtaca
    39901 attatttaag ctgcaagtgg tcacttcatg aggagtgttt cccttaccac tcaagaaact
    39961 caacagcaac cgttgatgtc agccagaccc cgttttccgc ttgccagaaa gggatgcctc
    40021 gtttcgccat ttcctgtgct ttgacggtta agataaccgg cgatccgtgt cttgctccca
    40081 ctttgcgtgc cgttgcttca tcagcagaga gatgtacgta atgacgctcc cctgcaatca
    40141 gcccctgttt ttttatctcg tcaaggaaac ggcttgccgt accgtggtag agaaactgtg
    40201 gcggtgtttt ttcggcaaag gaaatggcga cctgcgaagt cgaatgcccc tgaaccgcgc
    40261 ggatgcacct accatcactg gaataactaa aacgcttttt atcgctggtg gcaaccacag
    40321 tatccagcaa tgcacgggtt aaccgcttgc ccgctttttg ggcacaaagt atgagcttat
    40381 cgatatccgc ccatccttca cggtcaagta cgataccaat cgcctccggt ttgtgtcgga
    40441 ggacaaaact taaaaacttg ctcgtatccg ccaactcttt ttcgttgtac tttgccatcg
    40501 cgttttcgtc cctggatttt ctgtttttat ttactgatgt cattgtcact gggtttatga
    40561 attttctgca actcaatcct gcgcagagcc ttttctacac aacgtaattt ttccgctatg
    40621 caaaaaacgc atagcaagaa acatacttgc attttcccca tacccacatg gctgataagg
    40681 tttttcagct ccaggaattt tcctggctca agcaacacac aaacacaaaa aaacaatggg
    40741 gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag aaaaagtggg
    40801 cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggag ttttttccgg
    40861 gacagacagc tggtggcgcg tttttgattt ttccgttctg aacggatcgt ttgggcaact
    40921 gcctggtgcg aatggagcca ccacctcatt tcgcggggcg ggtggcgcgg gggctaaaga
    40981 tggctttctc tttgcgctgg agctggcacc gtcagtcatt ctctctctgg ggattatttc
    41041 aattaccgat ggtcttggtg gattacgcgc cgcgcagcag ttaatgacgc ctgtgctaaa
    41101 accgttgttg ggcattccag ggatctgctc gctggcgctg atcgctaacc tgcaaaacac
    41161 tgatgccgcc gccggtatga ccaaagagct ggcgcaggaa ggggaaatta ccgaacgcga
    41221 taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc atcatcacca actacttctc
    41281 ttccggcgtc gcggtgttcg cctttctggg cacgtccgta atcgtacctt tagccgtcat
    41341 cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc tggcttaact tcgaagaacg
    41401 tcgcaaccca acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat
    41461 atgtttatcg atggcgcgcg ccgtggcttt actatcgcca cgaccaacct gctgccaaac
    41521 gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct cgactgggtg
    41581 gggcatatct gtgaacccgt aatggcgctg tggggattac ccggtgaagc ggcaaccgtc
    41641 cttttagccg cattaatgag tatgggcggc gcagtcggtg ttgccgccag tctggcgacc
    41701 gccggcgcat taaccggtca cgatgtcacc gttttactgc ccgcgatgta tttaatgggc
    41761 aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc caaatattac
    41821 ccgcatatca ttacggtctg cgtgattaac gcattgctgt cgatctgggt tatgcagctt
    41881 attgtttaac aaggagttac catgattgat tataccgcag ccggttttac cctgctgcag
    41941 ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc
    42001 aaaattatcg ccgttgccag caatatccct tctgacattg taccggactg cacggttgtc
    42061 gatctcagcg ggcagatcct ctgtcctggt tttattgatc aacacgtcca tttgattggt
    42121 ggtggcggcg aagcaggtcc cacgacgcgc acgccagaag tggcgctaag tcgcctgacg
    42181 gaagcgggcg tcacgtcagt ggttggtctg ctgggcaccg actctatctc tcgccacccg
    42241 gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg
    42301 accggcgctt atcatgtccc ttcccgcacc attacaggtt ccgtggaaaa agacgtggcg
    42361 attatcgatc gtgtgattgg cgtgaaatgc gccatctctg atcaccgttc tgccgcaccg
    42421 gacgtttatc acctggctaa tatggcggcg gaatcccgcg ttggcggttt gctcggcggt
    42481 aaacctggcg tcaccgtgtt ccacatgggc gacagcaaaa aggcgttaca gcctgtctat
    42541 gacctgctgg aaaactgcga tgtgccgatc agcaagttgc tgccgaccca cgttaaccgc
    42601 aacgttccgt tgtttgagca ggcgctggag ttcgcgcgca aaggcggcac tatcgatatc
    42661 accagcagca ttgacgaacc ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg
    42721 ggtattccgc tggcacgcgt caccctcagc tccgacggca acggtagcca gccgttcttc
    42781 gatgacgaag ggaatttaac ccatatcggt gttgccggtt ttgaaacgtt gctggaaacc
    42841 gtgcaggtgc tggtcaaaga ctatgatttc agtatcagcg atgccctgcg cccgctcacc
    42901 agtagcgtag ccggtttcct taacctgacc gggaaaggcg aaattctgcc aggcaatgat
    42961 gctgacttgc tggtcatgac gccagaactg cgcattgagc aggtatacgc tcgcggcaaa
    43021 ctgatggtca aagacggcaa agcctgcgtg aatggaacgt ttgaaacggc ttaaacggtt
    43081 gtcagccgcc cgaatggacg tagaatgccc gcaagggcgg ctgacagagt aaaacgtaat
    43141 ggatgactgt ggtgcgattt tgcataatat tgaaaccaaa tggctttatg attttctgac
    43201 cctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt cgcaacgtct cgcaaccggc
    43261 attcagccgc cgcatccgtg cgctggaaca ggcgattggt gttgaattgt ttaaccgcca
    43321 ggtaacgccg ctgcaactct cagaacaagg gaaaatcttc cattcgcaga tccgccatct
    43381 gttgcaacag ttagagagca atctggcaga gctgcgtggc ggcagcgatt acgcgcaacg
    43441 aaaaatcaag atagccgctg cacactctct ttccctcggg ctgttaccgt ccattatcag
    43501 ccagatgccg ccgctcttta cctgggcaat tgaagctatt gatgtcgatg aagcggtcga
    43561 taaactgcgt gaagggcaaa gtgactgtat tttttccttt cacgacgaag atttgctgga
    43621 agcgccgttt gaccacattc gcttatttga atctcaattg ttccctgtct gcgccagtga
    43681 cgaacacgga gaagcacttt ttaacctcgc gcagccacac tttccgttac tgaattacag
    43741 ccgcaactcc tacatggggc gattgattaa tcgcaccctg acgcgccaca gtgagttaag
    43801 tttcagcacc ttttttgtct cttcgatgag cgagctttta aagcaggttg ccctcgacgg
    43861 ctgtgggatt gcctggctgc cggagtacgc catccaacaa gaaattcgca gcgggaagct
    43921 cgttgtactg aatcgggacg aactggtgat cccgattcag gcctatgcat accgaatgaa
    43981 tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt
    44041 gcttagctga cattctccag catcgcacta caccaaccgc cattatccca gaccagatca
    44101 aacgttagcg gttcaccgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg
    44161 cagtacgctt ctttaaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt
    44221 gcagaacgca gctgacggca ataccagctc tggagatgtt tgtcgctctg gttatgcact
    44281 tgccactctg gcatgcctga aacaagtaat gacggtaatg ccgaatgagg agaattaaaa
    44341 agagtgagga gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt
    44401 tcaggtaaaa cagcctgaag tgattggcgc atcattttgc gcactcctga cgtttagcaa
    44461 catatgcggc ttgccctgaa caccgggcac cactgtattg atgctcattt tccatagcca
    44521 ttcccttttg ttcattacat ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag
    44581 tataaaacct tacttgccta tgaatatcta ctgacttttc aggaaaaaag tcatgatttg
    44641 ataaaaatca tcaaactcaa aaaaaaaccg ctagtatatt ctgaatattc cgaatagtta
    44701 cgtagatgaa aatacttgtc accgctgcta cttaattccg gtgggaaatt atttatttcc
    44761 gcataatcag ccatgtatta tttgaaacaa ttagtttgta aataatataa attgataaca
    44821 tatcaaatac atcaaaaata ccattcacag gcattattaa tatagacatc aatatagcaa
    44881 catcaaattc caattagtaa taagatatgc tttttaattg catgaaaata atttcaaatg
    44941 agcgtttttt gatactttag agtgtttcct accctttaat atcaccgtgt agagttgata
    45001 tcactacaac agaaacatgc atgtcatttg tttacttctc ttt
//
LOCUS       DAMZII010000022        44764 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZII010000022 DAMZII010000000
VERSION     DAMZII010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.022"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            217..1311
                     /gene="gcvT"
                     /locus_tag="POA77_001207"
     CDS             217..1311
                     /gene="gcvT"
                     /locus_tag="POA77_001207"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI6364673.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1335..1724
                     /gene="gcvH"
                     /locus_tag="POA77_001208"
     CDS             1335..1724
                     /gene="gcvH"
                     /locus_tag="POA77_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI6364674.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1843..4716
                     /gene="gcvP"
                     /locus_tag="POA77_001209"
     CDS             1843..4716
                     /gene="gcvP"
                     /locus_tag="POA77_001209"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDI6364675.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4982..5725
                     /locus_tag="POA77_001210"
     CDS             4982..5725
                     /locus_tag="POA77_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6364676.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5782..7215)
                     /gene="bglA"
                     /locus_tag="POA77_001211"
     CDS             complement(5782..7215)
                     /gene="bglA"
                     /locus_tag="POA77_001211"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDI6364677.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7260..7571
                     /locus_tag="POA77_001212"
     CDS             7260..7571
                     /locus_tag="POA77_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDI6364678.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7735..8394
                     /locus_tag="POA77_001213"
     CDS             7735..8394
                     /locus_tag="POA77_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI6364679.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8590..9570)
                     /gene="ygfZ"
                     /locus_tag="POA77_001214"
     CDS             complement(8590..9570)
                     /gene="ygfZ"
                     /locus_tag="POA77_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDI6364680.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9813..10079
                     /gene="sdhE"
                     /locus_tag="POA77_001215"
     CDS             9813..10079
                     /gene="sdhE"
                     /locus_tag="POA77_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDI6364681.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10060..10467
                     /locus_tag="POA77_001216"
     CDS             10060..10467
                     /locus_tag="POA77_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDI6364682.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10507..11028)
                     /gene="fldB"
                     /locus_tag="POA77_001217"
     CDS             complement(10507..11028)
                     /gene="fldB"
                     /locus_tag="POA77_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDI6364683.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11140..12036
                     /gene="xerD"
                     /locus_tag="POA77_001218"
     CDS             11140..12036
                     /gene="xerD"
                     /locus_tag="POA77_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI6364684.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12061..12771
                     /gene="dsbC"
                     /locus_tag="POA77_001219"
     CDS             12061..12771
                     /gene="dsbC"
                     /locus_tag="POA77_001219"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDI6364685.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12777..14510
                     /gene="recJ"
                     /locus_tag="POA77_001220"
     CDS             12777..14510
                     /gene="recJ"
                     /locus_tag="POA77_001220"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI6364686.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14601..15699
                     /gene="prfB"
                     /locus_tag="POA77_001221"
     CDS             join(14601..14675,14677..15699)
                     /gene="prfB"
                     /locus_tag="POA77_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI6364687.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15709..17226
                     /gene="lysS"
                     /locus_tag="POA77_001222"
     CDS             15709..17226
                     /gene="lysS"
                     /locus_tag="POA77_001222"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6364688.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17269..17817)
                     /gene="idi"
                     /locus_tag="POA77_001223"
     CDS             complement(17269..17817)
                     /gene="idi"
                     /locus_tag="POA77_001223"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI6364689.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17872..17943
                     /locus_tag="POA77_001224"
     CDS             17872..17943
                     /locus_tag="POA77_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364690.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17940..18065)
                     /locus_tag="POA77_001225"
     CDS             complement(17940..18065)
                     /locus_tag="POA77_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364691.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18067..19515)
                     /gene="uacT"
                     /locus_tag="POA77_001226"
     CDS             complement(18067..19515)
                     /gene="uacT"
                     /locus_tag="POA77_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDI6364692.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19951..21870
                     /gene="ygfT"
                     /locus_tag="POA77_001227"
     CDS             19951..21870
                     /gene="ygfT"
                     /locus_tag="POA77_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDI6364693.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21870..22358
                     /locus_tag="POA77_001228"
     CDS             21870..22358
                     /locus_tag="POA77_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI6364694.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22394..23761)
                     /gene="ghxQ"
                     /locus_tag="POA77_001229"
                     /gene_synonym="ygfQ"
     CDS             complement(22394..23761)
                     /gene="ghxQ"
                     /locus_tag="POA77_001229"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDI6364695.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23797..25113)
                     /gene="guaD"
                     /locus_tag="POA77_001230"
     CDS             complement(23797..25113)
                     /gene="guaD"
                     /locus_tag="POA77_001230"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDI6364696.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25131..26531)
                     /gene="xanQ"
                     /locus_tag="POA77_001231"
     CDS             complement(25131..26531)
                     /gene="xanQ"
                     /locus_tag="POA77_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDI6364697.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26696..29566)
                     /locus_tag="POA77_001232"
     CDS             complement(26696..29566)
                     /locus_tag="POA77_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDI6364698.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRSEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29563..30342)
                     /locus_tag="POA77_001233"
     CDS             complement(29563..30342)
                     /locus_tag="POA77_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDI6364699.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30393..31721)
                     /gene="ssnA"
                     /locus_tag="POA77_001234"
     CDS             complement(30393..31721)
                     /gene="ssnA"
                     /locus_tag="POA77_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDI6364700.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31724..34822)
                     /gene="ygfK"
                     /locus_tag="POA77_001235"
     CDS             complement(31724..34822)
                     /gene="ygfK"
                     /locus_tag="POA77_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDI6364701.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35144..35722)
                     /gene="mocA"
                     /locus_tag="POA77_001236"
     CDS             complement(35144..35722)
                     /gene="mocA"
                     /locus_tag="POA77_001236"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDI6364702.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            35825..36595
                     /gene="yqeC"
                     /locus_tag="POA77_001237"
     CDS             35825..36595
                     /gene="yqeC"
                     /locus_tag="POA77_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDI6364703.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36643..38268
                     /locus_tag="POA77_001238"
     CDS             36643..38268
                     /locus_tag="POA77_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDI6364704.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38309..39241)
                     /gene="arcC"
                     /locus_tag="POA77_001239"
     CDS             complement(38309..39241)
                     /gene="arcC"
                     /locus_tag="POA77_001239"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6364705.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39289..40674)
                     /gene="hyuA"
                     /locus_tag="POA77_001240"
     CDS             complement(39289..40674)
                     /gene="hyuA"
                     /locus_tag="POA77_001240"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDI6364706.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40727..41938)
                     /locus_tag="POA77_001241"
     CDS             complement(40727..41938)
                     /locus_tag="POA77_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDI6364707.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAISIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41996..43192)
                     /gene="dpaL"
                     /locus_tag="POA77_001242"
     CDS             complement(41996..43192)
                     /gene="dpaL"
                     /locus_tag="POA77_001242"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDI6364708.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43250..44437)
                     /gene="ygeW"
                     /locus_tag="POA77_001243"
     CDS             complement(43250..44437)
                     /gene="ygeW"
                     /locus_tag="POA77_001243"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDI6364709.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMFLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10858 a        11265 c        11823 g        10818 t
ORIGIN      
        1 tttttggttt tttattctgt tgcgattttg gcgtttttta accataagct aatgtgatga
       61 tcaattttac cttatggtta acagtctgtt tcggtggtaa gttcaggcaa aagagaacga
      121 ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg tggtgataat ttcaccatga
      181 aaaagttgtc agccccgctt attcaatgag gacaagatgg cacaacagac tcctttgtac
      241 gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc acggctggat gatgccgctg
      301 cattacggtt cgcaaatcga cgaacatcat gcggtacgta ccgatgccgg aatgtttgat
      361 gtgtcacata tgaccatcgt cgatcttcgc ggcagccgca cccgggagtt tctgcgttat
      421 ctgctggcga acgatgtggc gaagctcacc aaaagcggca aagcccttta ctcggggatg
      481 ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct actactttac tgaagatttc
      541 ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc tctcctggat tacccaacac
      601 gctgaacctt tcggcatcga aattaccgtt cgtgatgacc tttccatgat cgccgtacaa
      661 gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg atgctcagcg tcaggcggtg
      721 gaagggatga aaccgttctt tggcgtgcag gcgggcgatc tgtttattgc caccactggt
      781 tataccggtg aagcgggcta tgaaattgcg ctgcccaatg aaaaagcggc cgatttctgg
      841 cgtgcgctgg tggaagcggg cgttaagcca tgcggtctgg gcgcgcgtga cacgctgcgt
      901 ctggaagcgg gcatgaatct ttatggtcag gagatggacg aaactatttc tcctttagcc
      961 gccaacatgg gctggactat cgcctgggaa ccggcagatc gtgacttcat cggtcgtgaa
     1021 gccctggaag tgcagcgtga gcatggcaca gaaaaactgg ttggtctggt gatgaccgaa
     1081 aaaggcgtgc tgcgtaatga actgccggta cgttttaccg atgcgcaggg caaccagcat
     1141 gaaggcatta tcaccagcgg gactttctcc ccgacgctgg gttacagcat tgcgctggca
     1201 cgcgtgccgg aaggtattgg cgaaacggcg attgtgcaaa ttcgcaaccg tgaaatgccg
     1261 gttaaagtga cgaaacctgt ttttgtgcgt aacggtaaag ccgtcgcgtg atttactttt
     1321 ttggagattg attgatgagc aacgtaccag cagaactgaa atacagcaaa gaacacgaat
     1381 ggctgcgtaa agaagccgac ggcacttaca ccgttggtat aaccgaacat gcccaggagc
     1441 tgttaggcga tatggtgttt gttgacctgc cggaagtggg cgcaacggtt agcgcgggcg
     1501 atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga catttatgcg ccagtaagcg
     1561 gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc ggaactggtg aacagcgaac
     1621 cgtatgcagg tggctggatc tttaaaatca aagccagcga tgaaagcgaa ctggaatcac
     1681 tgctggatgc gaccgcatac gaagcattgt tagaagacga gtaacggctt tattcctctt
     1741 ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac cctcactctc ttcgtaagga
     1801 gagaggttca caattcactg cacgtttcag gaaccatcgc tcatgacaca gacgttaagc
     1861 cagcttgaaa acagcggcgc ttttattgaa cgccatatcg gaccggacgc cgcgcaacag
     1921 caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg cgctgaccgg ccagattgtg
     1981 ccgaaagata ttcagcttgc gaccccgccg caggttggcg caccggcgac cgaatacgcc
     2041 gcactggcag aactcaaggc tattgccagt cgcaataaac gcttcacgtc ttacatcggc
     2101 atgggttaca ccgccgtgca gctaccgccg gttatcctgc gtaacatgct ggaaaatccg
     2161 ggctggtata ccgcgtacac tccgtatcaa cctgaagtct cccagggccg ccttgaagca
     2221 ctgctcaact tccagcaggt aacgctggat ttgactggac tggatatggc ctctgcttct
     2281 cttctggacg aggccaccgc tgccgccgaa gcaatggcga tggcgaaacg cgtcagcaaa
     2341 ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc atccgcaaac gctggatgtg
     2401 gtccgtactc gtgccgaaac ctttggtttt gaagtgattg tcgatgacgc gcaaaaagtg
     2461 ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg ttggcacaac cggtgaaatc
     2521 cacgactaca ccgcgcttat tagcgaactg aaatcacgca aaattgtggt cagcgttgcc
     2581 gccgatatta tggcgctggt gctgttaact gcgccgggta aacagggcgc ggatattgtt
     2641 tttggttcgg cgcaacgctt cggcgtgccg atgggctacg gtggcccaca cgcggcattc
     2701 tttgcggcga aagatgaata caaacgctca atgccgggcc gtattatcgg tgtatcgaaa
     2761 gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga ctcgcgagca acatatccgc
     2821 cgtgagaaag cgaactccaa catttgtact tcccaggtac tgctggcaaa catcgccagc
     2881 ctgtatgccg tttatcacgg cccgattggc ctgaaacgta tcgctaaccg cattcaccgt
     2941 ttgaccgata tcctggcggc aggcctgcaa caaaaaggtc tgaagctgcg ccatgcgcac
     3001 tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg gcgtactgac gcgtgccgaa
     3061 gcggctgaaa tcaacctgcg tagcgatatt ctgaacgcgg ttgggatcac ccttgatgaa
     3121 acaaccacgc gtgaaaacgt aatgcagctt ttcaacgtgc tgctgggcga taaccacggc
     3181 ttggacatcg acacgctgga caaagacgtg gctcacgaca gccgctctat ccagcctgcg
     3241 atgctgcgcg acgacgaaat cctcacccat ccggtgttta atcgctacca cagcgaaacc
     3301 gaaatgatgc gctatatgca ctcgctggag cgtaaagatc tggcgctgaa tcaggcgatg
     3361 atcccgctgg gttcctgcac catgaaactg aacgccgccg ccgagatgat cccaatcacc
     3421 tggccggaat ttgccgaact gcacccgttt tgtccgccgg aacaggctga aggttatcag
     3481 cagatgattg cgcagctggc tgactggctg gtgaaactga ccggttacga cgccgtttgt
     3541 atgcagccga actccggcgc acagggcgaa tacgcgggcc tgctggcgat tcgtcattat
     3601 catgaaagcc gcaacgaagg gcatcgcgat atctgcctga tcccggcttc tgcgcacgga
     3661 actaaccccg cttctgcaca tatggcagga atgcaggtgg tggttgtggc gtgtgataaa
     3721 aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg aacaggcggg cgataacctc
     3781 tcctgtatca tggtgactta tccttctacc cacggcgtgt atgaagaaac gatccgtgaa
     3841 gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc ttgatggcgc gaacatgaac
     3901 gcccaggttg gcatcacctc gccgggcttt attggcgcgg acgtttcgca cctcaacctg
     3961 cataaaactt tctgcattcc gcacggcggt ggtggtccgg gtatgggacc gatcggtgtg
     4021 aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg tgcaaatcga aggcatgtta
     4081 acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg cctctatcct gccaatcagc
     4141 tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa aagcaagcca ggtggcaatc
     4201 ctcaacgcca actatattgc cagccgcctg caggatgcct tcccggtgct gtataccggt
     4261 cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc gcccgctgaa agaagaaacc
     4321 ggcatcagcg agctggatat tgccaagcgc ctgatcgact acggtttcca cgcgccgacc
     4381 atgtcgttcc cggtggcggg tacgctgatg gttgaaccga ctgaatctga aagcaaagtg
     4441 gaactggatc gctttatcga cgcgatgctg gctatccgcg cagaaatcga ccaggttaaa
     4501 gccggtgtct ggccgctgga agataacccg ctggtgaacg cgccgcacat tcagaacgaa
     4561 ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg cagtattccc ggcaggtgtg
     4621 gcagacaaat actggccgac ggtgaaacgt ctggatgatg tttacggcga ccgtaacctg
     4681 ttctgctcct gcgtaccgat tagcgaatac cagtaattca ctgattcgac tatcttctaa
     4741 aggcgcttcg gcgccttttt agtcagatga caaagtacaa aagcgctcag acagtcccct
     4801 cacccctttg gggagagggt tagggtgagg ggaacaggcc ggcactggcg cgaatattta
     4861 ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa gcgtgccaac attgaagatt
     4921 gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct cggaaaaaca
     4981 aatggctata gcacttgtga ccggtggcag tcgcggcatc gggcgggcaa ctgcattact
     5041 gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc acgcggcgca
     5101 ggaagtgatg aacttaataa cgcaagccgg tggcaaagca ttcgtgctcc aggcggatat
     5161 cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg atgaaccgct
     5221 agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg aaaaccttac
     5281 cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc tctgctgccg
     5341 cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta tcgtcaatgt
     5401 ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg cggcatcgaa
     5461 aggggcgatt gatacgttaa ccaccggact atcgctggaa gtcgccgcgc aggggatccg
     5521 cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg gcggcgagcc
     5581 tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac aggcagaaga
     5641 ggtcgcgcag gccattgtct ggctactaag tgataaagcc tcttacgtca cgggaagttt
     5701 tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg caacaagtat
     5761 tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca cctcttggta
     5821 ccagttaaag ctcttcttac gtgaacgcga catatcgcca gcaccgtcgt catgtttatt
     5881 cacatagata aagccgtagc gtttgctgta ctgcccggtg gtgaacgata cgcagtcgat
     5941 gcagccccac ggtgtgtagc ccatcagatc cacgccatcg taagtcaccg cttttttcat
     6001 ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc tgccatcttc
     6061 ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc tctgataacg
     6121 ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc agccccagtc
     6181 agacgcttta acatacgggt tcggtacgct gccttcgaaa ccagagatcg catcgccggt
     6241 gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat aatcgcaggt
     6301 gccttcacgc agtacatcga gatcgccgtc ttccattttg atgttaaatc cgcgacgctc
     6361 ccactcgttc aacacatagg acgggtaata gccgcgtagc tgcacatcgg taaagacgta
     6421 gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacagc agtagggata
     6481 gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc gacgcgcagc
     6541 tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc acctgataca tcgtctcttc
     6601 cgggttctca tgctcggtat acaccacgcc ggagcagcag taaccgaaca gcggtgcacg
     6661 ccagttacgc tggttgttaa tttcgttgaa ggtcatccag tatttgactt tatgcttgta
     6721 gcgttcgaat accacttccg cgaaacggac aaagaaatca acgactttac ggttggtcca
     6781 gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg ttatcaccgg
     6841 ttcgatgttg tatttcagca gttcatcgaa catatcatcg tagaacttca gcccttcttc
     6901 gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg aagtccgaaa
     6961 acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac cataaaaatc
     7021 aacggcttca tggtttggat agtatttgcc tggcaagact tctttggtga tttcgcgcgg
     7081 cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggccctttgc cgcctttgtt
     7141 ccagccgcct tcgacctgat gagcggcaac tgcgccgccc cataagaaat ctttcggtaa
     7201 ggtgagtttt ttcactatca taggctccag atgagtcaat tcttacagga aatagtacca
     7261 tgcagccaaa cgacatcact ttttttcaac gtttccagga tgatattctg gctgggcgta
     7321 aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat gtgcttcgtg
     7381 tcgggcgttt tgaagatgac ggttattttt gcacgattga agtcaccgca acctcaaccg
     7441 taacgctgga tacgctgacg gaaaaacatg cagagcagga aaatatgacc ctgactgaac
     7501 tgataaaggt cattgccgac atctatcccg gtcagacaca gttttatgtg attgaattta
     7561 aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat ttcatgattt
     7621 ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac tggaactatt
     7681 ctcagttacg ctggagaggt atacatgtgc gtgtatgttt ctccggagta agttatggtt
     7741 cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa cagcgtcagt
     7801 cacggcattg ggttggtgtt tggtatcgtt gggctggtgt tgctactggt tcaggcggtg
     7861 gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg cagtatgatc
     7921 ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc aaaaatgtgg
     7981 ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta cacgccgttt
     8041 ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat ctggagcctg
     8101 gcattgctgg gtattctgtt taaactgacc atcgcgcacc gattcaaaat tttatctctg
     8161 gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat ggcagttaag
     8221 ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc gctcggggtg
     8281 attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg cttcgtgctc
     8341 ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc gtaatgggta
     8401 tgcctttgta ggcctgataa gacgcgccag cgtcgcatca ggcatcggag cgcaaccgcc
     8461 ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg ataaaggcat
     8521 caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg cctgatgcga
     8581 tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg tattcgcatc
     8641 gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga cctgtaccac
     8701 gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac gccagttctc
     8761 gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcgc tacctgccag
     8821 caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca tctcttgtcc
     8881 ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg tcgcctgtgg
     8941 gataaactgc ccgctgttgg cggcatcaat caccgggaaa cccgcttcaa tgtttaatgc
     9001 cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt
     9061 agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa accatagcaa
     9121 agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc taaagagatt
     9181 tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct cgtcgtctgg
     9241 cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct gcggttcacg
     9301 cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac gtaaattgct
     9361 ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt ctgccatctg
     9421 gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc cagtaatggt
     9481 ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag aagccgtagg
     9541 ctgacgggga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt aacggcgtat
     9601 taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac gcgatctgtg
     9661 ccttctgcga ggcgacttcc aaaaaaatga agccattgaa ttgttgtgaa ttaatttcgc
     9721 gatcgcgatg gtgtttactg atattcccgt aaaagaaggc gaaaaacacg ttacaatagc
     9781 cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa gcccgcattc
     9841 attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg tttttcgaac
     9901 atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg ctggaatgtg
     9961 acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat gcagaactgg
    10021 aaatgatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg gcaatctgat
    10081 ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct ggttgccgct
    10141 gttattttac tcatgccctg gccactcagt tacaccccgt tatggatggt gttactttcg
    10201 ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta atgctcgcca gggggaaatt
    10261 cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggtgcat cgtcaaagca
    10321 ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg taaacggcaa
    10381 catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt acggcggatt
    10441 ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc taagccgtca
    10501 gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc agctctgaat
    10561 acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca cgaacagttg
    10621 cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg gccagtagcc
    10681 gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg catcgaggaa
    10741 ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaacaattt taccttcaag
    10801 gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcctggattt caccaaaatc
    10861 ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg gggagtcgtc
    10921 cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt tttctgccgc
    10981 catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa cgtaagcttc
    11041 tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca cggcacaata
    11101 aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga tctggcacgc
    11161 atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac
    11221 gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg gttgacgctg
    11281 gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga gggcgggtat
    11341 aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca gtatctttat
    11401 cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa attgccccag
    11461 cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc accattaatt
    11521 gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc taccgggctg
    11581 cgtgtctctg aactggtcgg actgacaatg agtgatatca gcctgcgtca gggcgtagtg
    11641 cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt taggtgaaga ggcggtttac
    11701 tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt gtcaattgac
    11761 gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg gcaccgcatt
    11821 aaacattatg ctgtgctggc gggtatcgac agcgaaaagc tgtcaccgca tgtattgcgt
    11881 cacgcttttg ccacccattt attaaatcat ggtgcggatt tacgcgtggt gcagatgcta
    11941 ctgggccaca gcgacctctc caccacgcaa atttatacgc atgtcgctac cgagcgtctg
    12001 cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac gggaagattt
    12061 atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggttt tgctcaggct
    12121 gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag cgatattcag
    12181 cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggcgtgtt gtacatcacc
    12241 gatgatggta aacatatcat tcaggggcca atgtatgacg ttagcggcac ggctccggtc
    12301 aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt
    12361 tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac ctgtggttac
    12421 tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac cgtgcgttat
    12481 cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa agctatctgg
    12541 tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca
    12601 gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct tggcgttagc
    12661 ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca gccgccgaaa
    12721 gagatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata attcgcgtga
    12781 aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg cccgctgaat
    12841 tgcctccctt gctgcgccgt ttatacgcca gccggggcgt gcgcagtgcg caagaactgg
    12901 aacgcagtgt taaaggtatg ttgccctggc agcaactgag cggcgtcgaa aaggccgttg
    12961 agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggc gattttgacg
    13021 ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt ggttgcagca
    13081 atatcgacta tctggtacca aaccgtttcg aagacggtta cggcttaagc ccggaagtag
    13141 tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac ggtatttcct
    13201 cccatgcggg cgttgaacac gctcgctcgt tgggcattcc ggttattgtt accgatcacc
    13261 atttgccggg cgaaacatta cccgcagcgg aagcgatcat taaccctaac ttgcgcgact
    13321 gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg atgctggcgc
    13381 tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca attcctaacc
    13441 tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg ccgctggacg
    13501 ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc
    13561 cggggattaa agcgctgctg gaagtggcaa atcgcgatgc gcaaaaactc gccgccagcg
    13621 atttaggttt tgcgctgggg ccacgtctca atgctgccgg gcgactggac gatatgtccg
    13681 tcggtgtggc gctgttgctg tgcgacaaca tcggcgaagc gcgcgtgctg gcaaatgaac
    13741 tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaagtt gaagccctga
    13801 ccctgtgcga gaaactggag cgaagtcgcg acacgctacc cggcgggctg gcaatgtatc
    13861 accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgcatcaaa gagcgttttc
    13921 accgtccggt tatcgccttt gcgccagcag gtgatggtac gctgaaaggt tccggtcgct
    13981 ccattcaggg gctgcatatg cgtgatgcac tggagcgatt agacacactc taccctggca
    14041 tgatgctgaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa gaggataaat
    14101 tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggac ccttcgctat
    14161 tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc atggaagtgg
    14221 cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg ctgtttgacg
    14281 gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag gtgatggtcg
    14341 aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat accgctctct
    14401 ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa gctcgatatc aacgagtttc
    14461 gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag cgtcatcttc
    14521 tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat tttgactagc
    14581 tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc attcaggacc
    14641 tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa gaaagagcgt
    14701 ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc cgaacgcgca
    14761 caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct cgaccaaatg
    14821 aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa
    14881 gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact ggcgcagctt
    14941 gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgctacct cgatattcag
    15001 gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg tatgtatctg
    15061 cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg
    15121 gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg ctggctgcgt
    15181 acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg cggtcgtcgc
    15241 cacacatcgt tcagctctgc gtttgtttac ccggaagttg atgatgatat tgatatcgaa
    15301 atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg cggtcagcac
    15361 gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc caaccgggat cgtgacccaa
    15421 tgccagaacg accgttccca gcacaagaac aaagaccagg ccatgaagca gatgaaagcg
    15481 aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat ggaagataac
    15541 aaatccgaca tcggctgggg cagccagatt cgttcttatg tccttgatga ctcccgcatt
    15601 aaagatctgc gtaccggggt agaaacccgc aacacgcagg ccgtgctgga cggcagcctg
    15661 gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat gtctgaacaa
    15721 cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag
    15781 aagctggcga acctgcgcga gcaggggatt gccttcccga acgatttccg tcgcgatcat
    15841 acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact ggaagcgctg
    15901 aacatcgaag tcgccgttgc tggccgcatg atgacccgtc gtattatggg taaagcgtct
    15961 ttcgttaccc tgcaggacgt tggcggtcgc attcagctgt acgttgcccg tgacgatctg
    16021 ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat cctcggcgcg
    16081 aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac cgagctgcgt
    16141 ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca ggatcaggaa
    16201 gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg taacaccttt
    16261 aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg cggctttatg
    16321 gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg tccgtttatc
    16381 acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga actgtacctc
    16441 aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac
    16501 gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta catggcttac
    16561 gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct ggcacaggat
    16621 attctcggta agacggaagt gacctacggc gacgtgacac tggacttcgg taaaccgttc
    16681 gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga catggcggat
    16741 ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca cgttgagaag
    16801 agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga agcacatctg
    16861 attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc gcgtcgtaac
    16921 gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg tgaaatcggt
    16981 aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttctt ggatcaggtt
    17041 gctgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta cgttaccgca
    17101 ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg tatggtaatg
    17161 ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat gcgtccggta
    17221 aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt aattgtgctg
    17281 cgcgaaagca gacaacaatt ttcttgcttc actattggct gcctgcatca ccatccacgg
    17341 actgaacgcc cacggtgtgg catcaatacc gtgtaataca tctgctaaat cacaccattg
    17401 ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg cggcaaatac
    17461 cggacacact tcattttcca caatgccatt cggatcggtg gcgcgatagc gaaagtcagg
    17521 atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac ggcggatcac
    17581 cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa accgagttag tccacacgcc
    17641 aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgtcctt tggcattaaa
    17701 cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc gtgtgtgcgg catacttttc
    17761 cagcgtacca gtgggaactc cctgtgcatt caataaaatg acgtgttccg tttgcataat
    17821 ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga aatgattaac
    17881 caagtgagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt aaaaaccatt
    17941 taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac acagtcagaa
    18001 atgacaggaa tagagagagt aaaaagcaac agcagactga atatagcgcg cattaaaaaa
    18061 ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc acatacgtac
    18121 tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag catgatgctg
    18181 atagccatta aagaagacat tcagcacaac ggcgctaagg gttgcgagca taatgccgct
    18241 atgcagcaac ggttgcagta cggccggtaa cttagaaaag aaatcgtgag agagcgtcgg
    18301 agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt tggtggtgta
    18361 gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca tcaccagccc
    18421 agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt ttggcaccat
    18481 gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat gaacgcgcgt
    18541 cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt taaacgtgcc
    18601 gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt gtgaagagag
    18661 tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga taaacacgat
    18721 gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca taccaaatga
    18781 catcggcgtg acaatcgcaa accatgaagc atcatgtagc ccggataaat tgacttcatt
    18841 catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga cgttggacat
    18901 aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacagcaa aggagatacc
    18961 taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc agtcaatacc
    19021 cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca gtggcgggaa
    19081 taaaggcatc aagcgaccga taagtggcgc taataatgtg gtgataaaac ccgcggcgat
    19141 agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg ctatcattgg
    19201 tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca taaagcggcc
    19261 gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg agctaatgag
    19321 catcgcaata gcttcttttg agaggcccag tcggtcacca atcataagag gaacagcgac
    19381 tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct ttcctggtga
    19441 taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg gaagttggga
    19501 atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc aaccgtttac
    19561 gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag gaaggggagt
    19621 tagagacagg aattgcggag gcaatcacat aatcgtggtt aaaccacgaa aatgagaaga
    19681 gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta atcattctgg
    19741 catgtcgcac tctcgcattt aatcgttttt atctggatag cgctcttttg atcggcaatg
    19801 ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt cgcaaaatta
    19861 taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga aatttgagaa
    19921 gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc tgaagctgcg
    19981 gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa tcaagaaaac
    20041 tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa aggccaggct
    20101 gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gtgttacggc ttgtccggtt
    20161 aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg tattggctgt
    20221 aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac gattgcgcag
    20281 aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga agtctgccca
    20341 acacaggcgt tacgactgat ggacgataaa gggttacagt ggataaaggt ggctcgccag
    20401 cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag tgcagcgttg
    20461 ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg gaaaacccac
    20521 tttggcgaaa tctattgcgg gctggatcca ctgcaagcga cttatgagag tgaccgctgt
    20581 gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa cgctattccg
    20641 gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact ttgccaccag
    20701 accagttcct taccagaaat ctgcggcagg gtatgcccac aggaccgtct ttgtgaaggc
    20761 gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga acgctacatc
    20821 accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt tccccgtagc
    20881 gaaaaagtgg cggtgattgg cgctgggcca gcagggttag ggtgtgctga tattctggcg
    20941 cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg tatgctgact
    21001 tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg agagatattc
    21061 accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gccgcgatat tacctttagc
    21121 gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg gatgatgcga
    21181 gcagatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt cctgactgcc
    21241 catacccgcc agctcatggg attgccggag tctgaagagt atccgctgac ggacgtggaa
    21301 ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt gcggacttcc
    21361 atccgcctca atgccgccag cgtgacctgc gcgtatcgtc gtgatgaagt cagtatgccg
    21421 ggctcgcgca aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca gttcaatgtt
    21481 caaccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg cctgattcgt
    21541 accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt agcgggttca
    21601 gagtttgaat tgcccgccga tgttctgatt atggcctttg gtttccaggc gcatgccatg
    21661 ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca aaccggtgac
    21721 gtcgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg tgatgcagtt
    21781 catggcgcgg atctggttgt cactgcaatg gccgcaggaa ggcaggcggc gcgcgatatg
    21841 ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt aatccggctg
    21901 actgtattgg ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct tcagaacagg
    21961 agttgaatgc cgatgtcttt ttaccccggc tgaaggtaca gcgactggac agcatcagtg
    22021 cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc cccgtggggg
    22081 cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt ggctgtcaga
    22141 gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtctttgccg ggggataccc
    22201 ggcaacaaat cgtgaaatgc gatctctgtg aacagcggga agaggggcca gcctgcgttg
    22261 aatcttgccc aacacaggcg ctgcagttgc ttactgaaag agaactcagg cgagtccgcc
    22321 agcagcgtat tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg cgctgaaagg
    22381 cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt gcggcagtaa
    22441 tgatcaccgt accaatattc agcttttgcc attcgcgtgc aaagacgcgg cctacgacca
    22501 gtgtcacaaa gcccagcata ataccggtaa cgatattaca agtcagaacg atgaacacgg
    22561 cacacaccag gccagccata gcgtcaataa aatcattgaa atccagcttc gagacgttac
    22621 ttaacatcag caaacccacg tacatcagag cgggtgcagt ggcgtaacca gggatcaaaa
    22681 atgacagcgg tgataagaac agaatcagca ggaataacgc ccccactacg gttgcggtca
    22741 accctgtttt acccccggcg gcggttcctg ccgctgattc gatataaacc gctgcgggcg
    22801 ctgcgcccac cagaccggag aatattgaac ttactgagtc actggtcagg gctttgccgc
    22861 cattgatgat ctggttgtct ttatccaaca aattcgcctg accggcgacg gcacggatgg
    22921 tgccagtggc gtcgaacact gcggtcatca ccaatgccag tacactcgga agtacggttg
    22981 gctggagtgc gcccataata tcgaggctga aaatcagaga cttaccatct tcgccagtca
    23041 gacttggcat cgccaccaga ccgtggtatt tcaccgctgg gtcaaagatt aagccgataa
    23101 tcgaaattgc gataatcacc aacaagatcc cgccgggtac gcggcacttc tccagaccga
    23161 agatgaccgc cagccccagc aagctcatca tcaccgggaa ggaggtaaac gcaccgagcg
    23221 ccactggcaa gccttcaatc gggtttttga taaccatacc cacaccgtta gcagcaatca
    23281 gcagcagaaa caggccgata ccgatacctg taccgtgagc gatacccatc ggtaaattac
    23341 gtaagatcca ggtacgcaca ccggttacgg aaatagcggt gaagatgacg cccatcagaa
    23401 agaccgcgcc cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg
    23461 taaatgccgt caaggaaatc gcacaaccaa tcgccattgg caaattggcc cacaacccca
    23521 tcagcaacga gccgaagccc gcgaccagac aggtggcaac aaacacagct gcgggaggaa
    23581 aacctgcttt gcccagcatt cccggaacga cgataacgga ataaaccatg gccagaaagg
    23641 tcgttaagcc agccagtact tcctgacgaa cggtactgcc acgagcggta attttaaaat
    23701 aattatccag cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg tctccagaca
    23761 taatgatgtc ctcacgagga tgttaaagtt ttattattag ttgcgttcgt acaccagacg
    23821 accatcaacg taggtgcggt agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa
    23881 tttgtcgact aaagaaacag agttgtcata gcgcagctgc tgtagcggag tggcggtggg
    23941 ttccatcacc acgaaatcag cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag
    24001 gcccagagat ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg
    24061 atagccttgt aattgcaata ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt
    24121 ggttccggca ccgatatccg ttcccatgcc cactttaact ttcttctgcc atgctttttt
    24181 caagttgaat aagccgctgc cgaggtaaag gttggaggtc ggacagaaag caatgctgga
    24241 tttggtttcg ctgagacgat cccactcttt ttcttcgaga tggacgcagt gagcaaagac
    24301 acagttttta ccggtcaggc cgtactgatg gtaaacatcc agataaccat catggtcagg
    24361 ataaagcgat ttcacccagg caatttcatc tttgttttca cagagatggg tatgtaccca
    24421 cgtatccgga tattcttctt tcaggcgttg cgccatcgcc atctgttcag gagatgaggt
    24481 cggggcgaag cgtggcgtaa tcgcatatag cagacgacca tttttgtgcc agcgttcaat
    24541 cagttctttg ctttggtgat agctgctttc ggcagtgtcg agcagataat ccggtgcgtt
    24601 gcggtccatc atcaccttac cggcaatcat acgcatattg atatgactgg cggcttcaaa
    24661 cagcgcatca acagattgcg gatgaacagt gccaaacacc agcgccgtgg tggttccgtt
    24721 acgtaaaagc tgcttgatga agaatgccga catttcgcgg gcgtactcta aatcctcata
    24781 acgacgttca gtagggaagg tgtgtttatt caaccactcc agcaattgtt caccataggc
    24841 ccccaccatt tcactttgcg gataatggat atgtgtatcg acaaagcccg gtactatcag
    24901 tttgccgcga tagtcgcgca cgcgaatagt gtcaggaatt tgatgctttc cgtcttccca
    24961 ttcgccaaac cattccactt ttccctgttt aatgagtaat aaaccatcct caataaaccg
    25021 cagcgcagag gcaatctctt ccgggttatc gaccgtacgg gtgacatcaa taaaactgcc
    25081 tcgtaccgct ttcaacgtgt gttctcctga catcattgac tcctttactg ttaatccatc
    25141 tcttccgctg aggtaatacc aggcagaacg ttttcctgtc ggtagccacc agggaggata
    25201 atatttaata aaatcgcagt taacccgcca gcacaaatag ggttttcaac tagtacataa
    25261 atagaggctg gcaatatttt aaaaatttcg ggatcgtagg agacgccaag tcctaaacct
    25321 aaagaagtgg cgacaataag tgtttctcgg cgctttaaac cgttggtgat gattatgcga
    25381 atcccagcga tggcaatcat ggaaaacatc aacgtcattg cgcctcccag aactgccgag
    25441 ggaatggtcg tgaagaagcc gccaatcatc ggaaacaaac cgaggataac cagcattacc
    25501 gcgatggttc gcccgacata acgtgaagcg acgccagtca tctgaataac cccattattt
    25561 tgcgcaaacg tggttaaggg taatgacccg acagcggagg cgataacaga aaccagaccg
    25621 tctgccagca cgccgccttt cagtcgggac tgatactctt ctccctgaat gggtcggcgg
    25681 gaaaccattg ccgtggcggt gatatcgccg actgcttcca gcacgctaag cagataaatc
    25741 gtgccaacca ccaggaactg atggaagcta aaactaaagc cgtatttgaa cggatgcggg
    25801 atggtgatta acggcaaatt gcgcatactg ctgaaatcca ccatgcccag gcataacgat
    25861 gcgatatagc cgacacatag cccaatggcg atccctccca tgcgtagcaa cggactgcga
    25921 cagcagttaa agccgatcac cacaattaaa accaataaac caacgccgag atgttcgtaa
    25981 ttgccgaacg taccgctgct tttggctgca aatcctccac caaaatcgat aatgccgact
    26041 ttaatcaggc ttaagccgat catcagtacc acaataccgc tgacggtagg cgtaataacc
    26101 cgacgtaaat agggcaagat aaatgaagat ccgacaacca gaaatgcgcc aacgaaggag
    26161 acgccgagaa gcgacgacat gattaactct tcgtgaaaac cgtcgctttt catgctgctg
    26221 cccagcgcaa tcatgaccgt aacaaatgaa aaattgactg actgaattga gagtaggcca
    26281 gaaccgacga tgccgtagcg gtttacttgt aaccaggtac caataccaga ggcgatcatc
    26341 gccatagaaa caagataggc agttgtttca gcggaaagct gtaaggccgc ccccacgatt
    26401 aacgcggggg ttaccatcgg aacgaaaatt gccaacagat gggtaatggc accaacgaga
    26461 gcctgatgaa agggagggcg atcctccagt tcaaatataa ggtcagaacc tgcattgttt
    26521 atatcagaca tcccttcact ccttttaaag taaatttgat catctgaatt agtaaaataa
    26581 attcccacga atttggggcg caggacgcct tgagaacggc ttatctcatt ttcgcagtca
    26641 ctatttagtc atcgtccaaa cagagaaaca ggccgccgaa gcggccattt attatttata
    26701 ttttttccag cgcagtgagt attttctccg gtgtgaaatg ccattcgcgt aaccagatgc
    26761 cgcatgcatc gtgaattgcg gtagcaatcg ccggagctgc gccatttaca ccgatttccg
    26821 agatcgattt cgccccgaac gggccgactt tatcgtcgct cggcaccagc acagcgcgga
    26881 aatcacgcgg aatgtcacca attttcggtg cgccgtaact gcgtaaatca cgcgttaacg
    26941 ggtgaccttc ggcgtcataa atgatctctt cgctcatact gtggccgata gctcgcaggg
    27001 tagcaccgta gatttgtccc aatgctaatt ccgggttgac cggtgtaccg cagtccagca
    27061 aggcgtagaa tttatccagg cggatttcac ccgtacgcgt gttgacggca acttcagcga
    27121 agtttgcgcc atacgggaag gcgaaatcag gcgtgatata actgccagtt cccaccagtg
    27181 aaccaaagcc ggtgccggtt tcgcctttat gagcaatatc cccgaagctg acttcgcctt
    27241 tcttgccgcg cacgacgccc ggcgttgcca gttgaacatc tgccactgcc tcacccaaca
    27301 tttgcgcgcc gtggaacaga attttctccc gcagattttc cgctgccaaa cgcgccgcgt
    27361 tacctgagaa gcaagtaccg gacgaggcat atgcgccttt atcaaacaac gcatgatcgg
    27421 tatcaccgga gataacatgc acgtcctgcg gtgggcagtg cagcacttct gctgccagtt
    27481 tcgtcactac agtatccaga ccagtaccaa tatccgcacc gccagaatga acgataaagg
    27541 tgccgtccga ttccagtttg atcatgcagt tagcctgatc gatatccggg atacccgatt
    27601 tctgcatgat aatggcgacg ccgcgaccga tgtgccagtc accattttgc ggtttcggtg
    27661 acgaccattg gatcatctcg cgaccctgac gcaggatctc ttctagtgca cagctggcgg
    27721 cggaaggaac ggaggtcggc gctttacctt caccgattgc accgagaatt ttcagctctt
    27781 gcccttcgtg tacccggtta cgttcgataa tttccagttg gtcgatctgt aactgttcag
    27841 ccagttccgc taatgccatg gtgatagcga agttaccttt cggtgcgcca taaccctgat
    27901 aagcaccatt tgggcaaatg ttgctgtagt aggtggtgac ctggaaatcg acgttatcgc
    27961 acggatataa cggcagcgac agcgccggtc cgttacacgg tacggtgagt gagtggttgc
    28021 cgtaagggcc agtgttggcg cggaaatcca tcttcactgc cgtcaggcga ccatcttttt
    28081 tcgctcccag tttgacggtg actttcgcga cgtgacgaga ggtgttagca ataaactctt
    28141 cttcacgggt gtagcggaac aataccggac gcccggtcac gcaggttgcc caggcgcaca
    28201 cttcttccag caggatgtcc tgtttggaac caaaaccgcc gccaactcgc tctttaatga
    28261 catgaacttt atgctgtttc atgcccacga ggcgcgcgac ctggcggcgt aagtgccatg
    28321 gtacctgggt ggaggcgtgg ataaccagac gatcgccgtc catccgggta aagcagatat
    28381 gtgtttcagt cgggcactgc tgtgcctgcg ttgagttata ggttcgctca atgatcacat
    28441 cggcatcggc aaagcctttg tccatatcgc caatatgacc atgaatactg gcggcgatat
    28501 ttttgcgagg gcgagaaccg atcgggaagt tgatgatcat atgctcgctg cgctgggctg
    28561 catggctgtt atcgtcttcc agagtatctg gcgcaccagc aacatacacc accggttcat
    28621 cgtgcacgac aggcgcatct tccgccattg cttcgtcgat cgacattacc ggcttaagca
    28681 cttcatattc aacgtcgatg agcttcaatg cttcgagcgc aatttcttca ctttctgcga
    28741 cgaccgcagc aacacgatcg ccgacgtgac gcattttctt gccgaacata cggcggtcaa
    28801 gcggtgacgg ttccggtgcg ctctgaccac ccggggtata gtagatatcc gggcaattca
    28861 ggtgagtaat aacgtgaacg acgcccggta aggcttcagc tttgctgaca tccagatgag
    28921 taatcagtgc gtgagcgtgt gggctacgta acattttaat gacgcaggcg tcagccgtta
    28981 cgcggtcttc aacatagcag ggtttcgcct gcaccatttt cgcggcatca gttttaggat
    29041 aatgcttgcc aatgacttca aggtcgtcac ggaaagtcgg agcgatatca atggtggcct
    29101 gcggattatt tttacgtgca accgccagtt caatgacctg atagtattgt tgccagcctg
    29161 catcgcggct gaacaaacca gaaagcgcgt cgtcgatctc ttcgcgggta ggtgcggcga
    29221 tgcgatcgag aagatcggtg ataatcagag ctgcagctgg atcgttataa ccagactgca
    29281 ccacgccaac atcaaccatt gcctgttgaa ccagacttaa ctcgttccat ttgcccaaag
    29341 attctgcggt acgaatatct gccttctcta actgtgcggc aataagcaag gacgcgttaa
    29401 cgatattacc gttaaagatt attgcgtcag aaccggcaaa cccgaagcca tcatcactgt
    29461 tgcgtacaga gtgcattccc atgttaaaca acagcttttg cacgttttcg cctggattaa
    29521 cggttagctc ctgaggcgcg ccatttaaag taaagtggat gatcatacgg cttcctcccc
    29581 agcctgttgg cagtcggcat acaggtcggc taccagaact cccgtgatat agcgtttata
    29641 ggccacgctg ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag cctgttcaag
    29701 tgcattgcct tccagatttt gtttttcgac atcatgcaga cgcagtgctt tactggcgac
    29761 gccatccagc gcaattcgca taccgtcgtg gtctgtcatt gcaacggctg ccgtcacgac
    29821 ggttaaacct gcctgagaac ggctaatttt gcgggtcgca caggtgcgat acggatcttt
    29881 aatgataatt tcggttagca ggcgatcgca tgggcaggcc aggtagtcct cgattgacag
    29941 cgtttcgccg ttgccaaaaa ccagttcagc atccagtgcc aacaggacgg gaagcagcac
    30001 cgactcttcc tggcgggcgg cgatttcacc accaatggtc gactgattac gaacatggcg
    30061 tgagtaaaca aaaccgaggg cttcacgcag cgctgcagga ataaatcgcg catcacgcag
    30121 tggctgcaag cgagacattg cgccaatccg cagtgcaccg ttatcccagt caacccagtc
    30181 cagttccaga tcctgtaagg aaatggcaat ctttttatcg gtacgggttg gtgtagcatt
    30241 gagtttgctg cccccggcga accagacggc ttcatcctgg tagcggcgct tcagttccag
    30301 cgcctgttcg acagagtcgg gcctgaaaaa ttgttcaatc atcttctttc cttatttaac
    30361 cctcttccct gcgggaagag ggcatctgtc atttatgcca gcgcatccat ccgacgccac
    30421 atactggcag cggcttttct ggcttgtgca taaatggaat cgcaatcgaa gttaaactga
    30481 cggtcttcat agaccatcac accattgacc atcacgctgt gaacgctgcc tgagcccata
    30541 ccgaaagcga tatgcccggc gatattgtct gccagcagcg gcgtcggtga gttgtaatcg
    30601 caaatggtta aatcagcttt gtaaccggct tccagaagtc caaatttcgc gccaaagttg
    30661 cggctcatca gttcgttacc gttagtcagg gctttggcaa aactgtcagg ccacagcgga
    30721 ccacccgcat cgcgatgttt aaagaaggca aatttcatct cttcaaacat gtccgaacca
    30781 atgccgtccg ttcccaacgc cagattgcgg atgtcgctaa ggtgatggtt gtagccgaca
    30841 tggttgttca tgtttgaacg ggcgttatgc accaggaacg catcgcgctg attgagtagg
    30901 gcgatgtcat ctttcgacaa gtacagccca tgagcgacca gcgttttgct atcgatgaga
    30961 tcgaattgcg ccagtcgtgc cagcaggtct ttgccgtacc agtggtgact gtaggaaacg
    31021 tcgtaaaggt cttccgcagc gtgaatatgc aaaccacggc ctgtggcttt cacggcttca
    31081 cgtagcatct ccagaccggc atctggcacg gtaaacggcg cgtgagcacc gatatgtgct
    31141 tccaccagat acggctcggt tgctgctttc ttcgcctcat caatctgacg ggcgaagcgg
    31201 atgttttctt ctacaccttc ctgcaactct ttgatgccgt tgttacggtc agtagtttca
    31261 aaacaggtca tcgcgcgcag gccaactttt aaaaatgcgt cgcgcaatgt ggagagcgac
    31321 ccgccgatat acgccggaga ggcatggtga tcgataaccg atgtacatcc gctcttaatc
    31381 gcttccaggg aacaaatcag cccgctgtaa tagagcgact cttcatcaag ggcgcgatcg
    31441 agccgccacc agagattttt cagcgttgag atgaaatccg ggcagggggc gatgtttgcc
    31501 ataattccgc gggaaagccc cgagtaaaaa tggttgtgcg agcagacaat tcccggcatc
    31561 acaatccgac catgcatctc tttgtagctg gcatcggggt agcgttgcgt cagggcatcg
    31621 ccgatagcga caatcacatc attttcgatg gcgatatcaa cgccttcctg cactttcgcc
    31681 gggtgtaact gtactgcggt gacattcttc agaatcaaca tgattacacc tccacgcggc
    31741 ccagcagata atgatgatgc tggtggacat ggctgatgat gcggcacata tcgttcagct
    31801 ccggtggtac gttgtcgaac tggccttcgc tgtcgatgtt cagcacccag ctttggttat
    31861 tcagacgtac gcgtacccga caatcttcca ccaggaagcc tgggttgctg ctgttatcaa
    31921 agtcttgcga caggctgaag acggtgattt tgtctttgta cggtttaccg ttccacggac
    31981 agaactgagc gcagttgccg cattcgttac agtaagcgtc gaggtgcagc gtctggaaac
    32041 ggttctggaa gcctgggacc gcaatggaga cgttggcgcg gttcgggcag acatccacac
    32101 acttgctgca aacgtagtta cattcgaggc agcgagcggc ttcctgggcg acaaacgcat
    32161 cacgatcgtc actgttcacc agcgtgatag agatatcgcc tttacgttga tagatttccg
    32221 ccggattgac gttgttccag tatttattgt tctggtggga acggatattt tcccgactaa
    32281 ggatggcatc ggtcgcccga cgcgcggttc cgacagcagc gacaatggag gatggtccgc
    32341 gctgtacgtc gccgatcata aagacgtcag tcagacgagt ttcgccatta tggtcgacgt
    32401 ctggccagcc gtttttgtcc agcggtacgc ccatcgcatt cagggcttca gtatcctgct
    32461 gttcaccgat ggcggtgatc aggctgtcta catgcagtgt tactgtttcg ttggtttcaa
    32521 ccggacggcg acgacctttc tcatcgggtt cgccaagcga cataacgcgc aaggttaagg
    32581 tgccatcagc atcgaaacat tccggattat tcaggaaacg gaactccacg ccgtcgtgca
    32641 acgcttcttc atactcttcg cgccatgcgg gcatctcttg cagtgaacga cggtaaacga
    32701 tcgttgcttt ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc attgcggtgt
    32761 tacccgcccc gacaacgacc acatgtttgc ccagcttgag cgctgtaccc ttgttgtatt
    32821 cacgcaggaa ggggagtgat ttccagacat tttggttgtc gcccgccagt ttcacaccgc
    32881 tatttttatc agtgccggtg gcaatcagaa catagtggaa gccctgattt tttaactgtt
    32941 caacggtcaa atcgggtgag cagccatact caaatttcac gccgtgatcg gcaacaaaat
    33001 cgatatcgtg ctgaattaac tcagccggaa tacggaactg aggaatgata tttttcacca
    33061 cgccgcccgc attggcttcg cgttcaaaca gcgtaaccgg atggcccgct ctggcaagga
    33121 agtaacctgc tgccagaccc gccggacctg caccaatcac ggcaaccgga tggcgtgaac
    33181 cagaaccggc tggtttgtgc cagcgttgct tatattcatc ccaacctttt tccagcgcga
    33241 cttttttcag ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta cagttgtatt
    33301 ggcactggtg atcgcaaata tgaccggtaa tggcgggcag agcgttgcgt tggtagatga
    33361 gttccagcgc gtcggcatag cggtgttcgc caagcagacg gatgtattcc ggaatatctt
    33421 gcttgatagc gcaggcagta acacaggggg caacgtagca atcggtcagc ggcaggtctt
    33481 ctgccacttc aatacgctct tctggcttcc agtgtttctg ggtgtattcc atggttaacg
    33541 catctgctgc cagtctgttc agtcgttcga cgtcaacatg gtcaagtccc caggcgtcgg
    33601 agccttccag ctcgcgcatg caggcactta agcgcaaata gccgccaggt ttcagcaggt
    33661 cggttgccat agtaataggg cgaatacctg tatcaaaaat atcgcggata gtcagctgac
    33721 tggcaccacc ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag agaactgctg
    33781 caacattgat ggagagcggg aacagcgcac ggcctgacat atacatctct tcaccaggca
    33841 gtgcgccttt attgttgatg gtgccgagag tgttagtcag ttttacgcca aagccgagtg
    33901 atttttcttt tgccagtgcc atcaggcgtt ccagcatttc cagtgcttgc gtcagcttga
    33961 ggtcgtgatc aaatgactct tcttttaagc ctatgtaacc gaaaccgcag acatcgagga
    34021 tctcacgaac acgcgcgtat cccagtaagg tcgggttaag tttcacaaag gtgttgagcc
    34081 ctttttcttc cagcatgtag cggcaaatgg cttcgatttc atgcggagga cagccgtgca
    34141 tggtggagag ggtgacgccc tgcaccatac tggtggggat gcgagcgggt aaggcttgca
    34201 agctttcgcg tttttcctgc aatccgtggc gggctaaaaa tgcgtcatcc tggagtaatt
    34261 tattcagcgt atcgcgatat tgcgcgaatt tcggatggtc agatgcgtcc atcatattgt
    34321 cgatgaactg ttgcattggc ggttgcttaa taccttcgag gttgtaaccg acgctcatat
    34381 taaagatgaa cgatttaccg gaatcagaag gctggaacat cgcttcgaga aggtgcaggg
    34441 caaaccaggc tttgaggtat tcatcccagg ctttaagcag ggtaaattcg gtagaccatt
    34501 cggtgttaaa gcactcgtct tcggcatcga tacagggctt ttccagctcc aggcggtcaa
    34561 gaatttggac ggtttttagt tcgatgaagc gtccgccagt cagccaggaa gtgacaatat
    34621 tttgcgcgag ctgcgtgtgc gggccagcgg cagggccgac gggagtggca caggtttcac
    34681 cgaagacgct aacagtttta ccttttacag gtgagtaaaa ctgttgctcg ggaataccaa
    34741 agattgagcg ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc tcaaacggaa
    34801 tgggacgcat aatatccccc ataaccctct ccttagctta tacagatatc attgagtgac
    34861 cggtgttgag aaatccggtg gtttaggggg atagagcaac aatcgcacca ctcttgccag
    34921 gtttggcggg aaatataaaa aaatgtgagg cggctcgaaa cttaaaggct ttttgtgaat
    34981 gaactcacag gtgccaaatg agaattaaaa tcactcctga aaattattgt tgtcagccca
    35041 tttatcactt tttctctttt ctcaaaatca gtgattcctt atcattatga taaagagtgc
    35101 tggagagatt aaatatttga acgcaatcgt ttaactacat tttctaaatt ttagtatacc
    35161 ttttttgcgc tgtaataaag tcgtccggag tatcaatatc taaaattatt tctgcatttt
    35221 ctatctcgac ggaataatga gctcccataa ggagtgcctg acgcatattg gtgacattgg
    35281 gtcgttggat tgcctgcatc aagcatggtt ttgacactaa aatcggatgg ccggggatgc
    35341 cattatggag tggcagtatt gcgccatcat ttcgtaacga ccagattttt ctaaaaatat
    35401 cgatggtgag ggttggcatg tcaccgtggg tgagaaaaca atgttctgtt tgcactgcgg
    35461 gtactgcggc ctttactgat gttagtaaac cctgcgcata atctgggttg tggataatag
    35521 tgatattgct ctggttcgca taacgttcgt gcagttcatt accacgatag ccggtgacta
    35581 aaataattcg gctacaaaac tgcaacgcat ttttgatact tgtatcaaga attgtcccct
    35641 gttgccaggg taacatcatt ttccattgcc ccattcttga tgataatccg gcagcggtaa
    35701 ttatacagtc gatggctgac atagtggttc cattatttat aggggctaat aacgcctgat
    35761 aattgcgtta cttcctgatg gattaagttg gggataattt atcccagaga ggtcataaag
    35821 actcgtgaaa agtatagttg acccatcggc attagtcatt gatttaggtg cgcagaaacg
    35881 tcccactgtg atttctgttg ttggtgctgg ggggaaaacc agcttgcttt tttggctggc
    35941 agaactgctc caggctagcg gcaggcgtgt gttaatcacg acgacgacac atatgtttat
    36001 gccaacgtct cactggcccg tggttttctg tcgtgatccc gccatgcttc ctcatgcgtc
    36061 tcttacatcc cccatttcat tttgttttca cagctggaaa gcgaaccagg gaaaagtgca
    36121 gggatttacg ccagaagcga ttgatgcact ggtgcaacga ccagagtgtg acgtaattct
    36181 cattgaagca gatggctcgc gtggaatgcc gttaaaagcg cctgatgagc acgaaccttg
    36241 catacccaaa agcagttgct gcgtgattgc tgtgatggga ggacatactt tgggcgcgaa
    36301 agtgagcaca gaaaatgtcc atcgctggtc gcagtttgct gatattaccg ggttaacacc
    36361 tgatgcaacc ttgcaactga gcgatctcgt tgcgctggtt cgccaccctc agggggcgtt
    36421 taaaaacgta ccgcaaggtt gtcggcgagt ctggttcatt aaccgttttt ctcaatgtga
    36481 gaatgcgatt gcacaaagcg agctccttca accgctgcaa caacacgacg tagaggcaat
    36541 ctggctgggc gatatacaag aacatcctgc aatcgcgcgc agatttgtga attagcggcc
    36601 cgggtattcc gggttactga aaggatatcc attgaggttg gtatgaatat tttcacagag
    36661 gctgcaaaac tcgaagagca aaattgtccg tttgcgatgg cgcaaattgt tgatagccga
    36721 ggctcgactc cccgccattc tgcacaaatg ttagtgcgcg ccgatggttc tatcgtcggt
    36781 acaattggtg gcggaatggt tgagcggaag gtgattgaag agtcacttca ggcattgcag
    36841 gaacgtaagc cgcgattatt ccatggacgt atggctcgta acggtgcgga tgctgtcggg
    36901 tcagattgtg gaggtgcaat gtcagtattt atcagcgtcc atggtatgcg tccacgtctg
    36961 gtgttgattg gcgcggggca tgtcaaccgg gcgatagccc agagtgcggc gctattagga
    37021 tttgatattg ccgttgccga tatttatcgc gaaagcctca atcctgaact attccctcca
    37081 tcaaccacgc ttctccatgc tgagtcgttt ggtgcagcag tggaagcact ggatattcgc
    37141 cctgataatt ttgtcctgat tgccacgaat aatcaggatc gtgaagccct cgacaaactc
    37201 attgaacagc ccattgcatg gttggggttg ctggcaagtc gccgcaaggt tcagcttttc
    37261 ctgcgtcaat tgcgtgagaa aggcgtggct gaagaacata ttgcccgttt acatgcgccc
    37321 gttggttaca acataggtgc ggaaacgccg caggagatcg ccatcagcgt gctggcagaa
    37381 atattacagg tgaaaaataa cgcgccgggt gggctgatga tgaaaccttc tcatccttcc
    37441 ggacaccagc tggtggtgat tcgcggtgcg ggagatatcg ccagtggtgt ggcgctacgt
    37501 ctgtatcatg cgggttttaa agtgatcatg ctggaagtgg aaaaaccgac agtgattcgt
    37561 tgtaccgtgg cgtttgccca ggccgtgttc gatggcgaaa tgacggtcga aggcgttact
    37621 gctcgcctgg caaccagctc tgcggaagcg atgaaactta ccgaacgcgg attcatccct
    37681 gtgatggcag atcccgcctg ttcattgctt gatgaactga aaccgctttg cgtggtggac
    37741 gctattctgg cgaaacagaa tttgggaacg cgggcagata tggcaccagt aacaatcgcg
    37801 cttgggccgg gctttactgc agggaaggat tgtcatgcgg taattgaaac aaatcgcggg
    37861 cactggctcg gtcaggtgat ttactctggt tgtgcgcagg agaataccgg tgttcctggc
    37921 aatattatgg ggcataccac ccgacgggtg atccgtgctc ctgctgcagg cattatgcga
    37981 tccaacgtga aattaggcga tctggtgaaa gagggcgatg tgattgcctg gattggtgag
    38041 catgaaatta aagcaccgtt gacggggatg gtgcgtggct tgttgaacga cggcctggca
    38101 gtggtcggtg gttttaaaat tggtgatatc gatcctcgtg gtgaaacggc tgatttcacc
    38161 agcgtttctg ataaagcccg ggcgattggc ggcggcgtac ttgaggcgtt aatgatgttg
    38221 atgcatcagg gcgtgaaagc gacaaaagaa gtgctggaag tggcttaaat gaaaatggcc
    38281 ggatggtatt acccacccgg ccttaaaatt actgacaaat caccgttccg gttttgcctt
    38341 caatcccctc tttcgctttg ctcaatacgg taatcagtgc ttcacgacct gcgcgagagc
    38401 gagcaaacga ggcggcagct tccactttag gcagcataga acctttcgca aaatgacctt
    38461 cttcgataaa gcgttcagca tcacttaatg acaggcgatc gagccactgt tcattctctt
    38521 taccgaagtt aatggccact ttttctaccg cagtcagaat gatcagcata tcggcatcaa
    38581 tcatttctgc taaacgggcg ctggcccagt ctttatcgat aaccgcgctg gcaccacgca
    38641 gatggttacc ttcacgaata acaggaatac caccaccgcc aacggtgatc accacctggc
    38701 ctgcatctac cagagcttta accgtttctt tttcaataat atcaaccggt tttggcgagg
    38761 caacaacacg gcgatagccg cgaccagcat cttctttcag agtgtaaccc tgttttgtca
    38821 gttgctctgc ttcttgctcg gtaaagaacg aaccgatcgg cttggtcggg ttgaggaatg
    38881 ccggatcgtt agcgtcgact tcgacctgag taaccagcgt tgctaccggt ttattaatgc
    38941 cccgagaaag cagttcttct cttaaggcgt tttgtagatc gtaaccaata taaccctggc
    39001 ttaaggcaac gcagacagac atcggcagca ttggcgagtg cgcttcagtt tttgcagcag
    39061 cttcaaaggc ctggttaatc atgcccacct gcggcccgtt accatgagtg acgataactt
    39121 catgtccttg ggcaattaaa tcaacaattg cctgagaagt aatttttacc gctttcattt
    39181 gtccggccag gtcatcgccc agcgcattcc cgcccagggc gagaacaatt ttcttactca
    39241 tttttcctca ctcatcattc ttgattgtgt atagatccct gacagtggtt agagcacggg
    39301 agggacaaac ggtttgcgtc gcaggaaacg acctcgcccg gctttgcctg taaaggtgcc
    39361 gtcacagaaa atcgtttcac cacgggataa ggttctgaca atcgcgccct gacaggtaaa
    39421 accctcccat ggcgagtagt cggcgttgtc gtggagatgg cgatgctgaa tttgttggct
    39481 ctgacgtggg tcgataatca ccacgtcgcc atcggaaccg ggcgctaata atcctttttg
    39541 cggccacagg ccaaacagcc tggcgggcat tgcgctggtt aattcaacaa agcgttccgg
    39601 tgttatacgt cccgtcatca cgccactgga aaacagtaac tgcatgcggt tttccacacc
    39661 gggtaagcca tttgggcagc gactgaaatc gcctttagaa atttgcaggc gttgagccat
    39721 cgagaaggtg cagtgatcgg tcgccaccac gtcaatcgca ccatcgctga tgccacacca
    39781 cagtttgtcc tgctcgcgta cgttacgcag cggtgggcta agaatgaact tcatgccgtc
    39841 ttctgtatcg taactgcgtt cgtccaacag gagatattgt gggcaggttt caacccagac
    39901 tggctggtga ttcgcacggg caagacgcag ataatccaga cctaagccgt tagacaggtg
    39961 cacgatatag agcggggcgt taccggcaat ttgtgccagg ttaatcatgc gggcgatggc
    40021 ttccgcttcg cattccagag ggcgactcaa ggcatgatag cgcggcgcgg ttaaccctgc
    40081 ggcgataaat tccgcccgct tgctggcgat agccgcatca ttttccgggt gcacggtggt
    40141 cagcgcgccg gattcatgca gacggcgtaa tgcctgcaaa acctcgtcat cgttgagttt
    40201 gtattgatag gttaaataga gtttaaaact gctcagtcct tcctcgacca tcatcgggat
    40261 ttcgtcgagg attgcgtgat taatgtgctg gatcacaccg tgaaagctgt aatcgatgac
    40321 cgctttatgg gcggcataac cacgataaac ctccagttga tggcgtaacc gacagccgtt
    40381 tgggccaaat cccatatggt caataatggt tgttgtaccg ccacacgcag ctgcgcgggt
    40441 accggtaaaa aaatcatcac aactgcgcgc gatgccgaca tcaatattga aatgcgtatg
    40501 gacatccacg ccgccaggga aaacgtaaca gccagtggca tcaatttctt catacgggag
    40561 ctgcggcgaa atattgttgc ccaactggcg aacaatcccg ctttcaatca gcaaatcctg
    40621 tttggcttgt ccatctgcgt taacgacagt gccgtttttg atcaatacgc gcatagcaaa
    40681 ctccaaaaac cctccggcga accggagggg aataagtgaa gagtaattac tctgttgcca
    40741 gccagcttaa cgggattgca gcgtacatcg cggcacaggt caccaggtga gatttccagg
    40801 ttttttcgtt aggtgcatgc gcttcaggtt ctttacccgg gccaaagccg atcaccggaa
    40861 tgccgtgacg gcccatgata gatacgccgt tagttgagaa ggtccactta tcaacaaccg
    40921 gcgctttgcc aaacagccct tcgtaggcat tcaccagtgc tttaacggtg aagtgatctt
    40981 cttccacttt ccaggtcggg aagtagcatt cggttgggta aaccaggcca gtccaggacg
    41041 gacggtcgta gttgtacata gaaacaaccg cgttagcttt ctgtactgca ggcagggcgc
    41101 ggatttcgtc cagcgcgcct tcccaggttt cgccccaggt cagacggcgg tcaatagaga
    41161 tggcgcagct atctgctaca gcgcaacggc ttggggatgt gaagaagatt tcagaaacgg
    41221 tgagggtgcc tttgccgagg aattcgtcat aacccagacg ttgggagagt tcttgtaatt
    41281 cgccaagaat cggacccatt ttgaaaatgg cgttgtcacc gcgttctggc gcagaaccgt
    41341 ggcagctaac accctgaaca tcaatacgaa tttccatacg accgcgctga ccacggtata
    41401 tctggcagtc ggttggttcg gtactgacca caaattccgg gcgaatgccg gattgttcaa
    41461 taatgtactg ccagcacaga ccgtcgcagt cttcttcctg cacagtaccg gtaaccagca
    41521 gggtatattc atcttccaga ccgaggtctt taatgatttt accggcataa accatagatg
    41581 ccatgccgcc ttcctggtcg gaagtaccgc gaccaccgat cagctcatca gtttccatgc
    41641 cttcgtacgg atcgaagtcc cagtttttga tgttgccaat gccgacggta tcgatatgag
    41701 cgtccattgc caccagacgc gggccgtggc cgatataacc gagaacgttg cccatcgggt
    41761 cgatttcaac tttatcgaag ccgacttttt ccatctcttc tttaatacga tgtactacgc
    41821 gtttctcgtc gcagctttca ctgggaatag caaccatgtc gcgcaggaag cgagtcatat
    41881 ccgcctggta atcttttgct ttttcaagaa tcagtttgaa tggaatattc ttagccatta
    41941 tttttctcca gattttttac tccccgtttt gcagggagca gttccaatag ggtgattaag
    42001 gtgctacagc gtgtttgcct tcccagacaa cttcgcggta gtgcttcacg tcggtgtcgc
    42061 cttcagtgct gataaccagc accacggcat ctttgttcag cgccagtttt tccatcaggc
    42121 tttgacgttg cgggtgataa tgaaccgctg cgagaacgcc caaaccgaca gcgccggatt
    42181 caccggagat gatgcgcggg tcgttgccgt acggattacc cagcacgcgc atacctaatg
    42241 cggcaacgct gtcctggcag gagatgaatt gggtggcgca gttacgtagg atttcccagc
    42301 ccagcgggtt aggttcgcca caggccaggc ctgccatgat ggtggccata tcaccgccaa
    42361 cgttgacgat gtcgcctttg acgccggagc gataaataca gtcagctttg tcaggttcaa
    42421 caataatgct gtgcagattt tgcgggctat agacgtcgac cagataaccc agcacaccac
    42481 cggccattgc tccgacaccg gcttgcagca gaacgtgcgt cggggttacg cccatttcac
    42541 gcatttgctc gacggcttca tctgccaggg ttgcgtagcc ttgcatgatc caggttggga
    42601 ttttggtgta accttcccat gccgtgtcct gtaccacttc ccagccgtgc tgctgcgcgt
    42661 gttgcatggt caggcgaacg gtatcgtcat agttcatatc tgtgacgatg cactcggcac
    42721 cgaggttcag aatggcgtca acgcgttcct gagcagaacc tttcggcatg taaatcaccg
    42781 cattctgtcc gagttgctgc gctgcccacg ccacaccgcg cccgtggttg ccgtcggtgg
    42841 tggtcgcgaa agtcattttt tcgccgatgg catttttcag gtgctcaaat gacagcgttt
    42901 cgatatcaag atgatatttt tcacacaata attgagcgat ggcgtacgca ccgccgagca
    42961 ttttgaaggc gttcagaccg aatcgttttg attcgtcttt gacaagaatt ttcttcacac
    43021 caaaaaggtt tgcgagatcg tccagcgcac aaagcggtgt tgggcgataa ccagctattt
    43081 tctggtggaa ctggcgcgcc agtttggcct ggctttgcga aaagagcggt gatgtttcgc
    43141 cgttgaaaaa tttgttatcg gcgatatcaa tcttcaatga gaaaacggac atatcctatc
    43201 cttttggcct ctggaaatag agggccgacc agctgatcgg cccgaggggt tatttcacgc
    43261 gttcttgcgc ttctttcagc agttgctcga gcagtgcacc tggtttggcg tatttacggg
    43321 acaggatcat cgcggcgatg atataaggct tccagctggc ttctttgtag gttgcgatac
    43381 ggtatttttc gaatacgcct tcagtcactt caccttcttt acaggatacg ccgctgatat
    43441 cagctggcag gcagtgcatg tacagggctt cgccatcacg ggtcagttcc atcatctctt
    43501 cagtacaatg ccagtctttg tgttgcgcgt tctgtgccag acactgtttt tccagtgctt
    43561 ttaagccttc gtgatcgtta gcgcgcagca attcggtacg ctgttccatc actttgtaag
    43621 gtgcccatga cttcggataa acgatgtctg catctttgaa cgcttcttcc atgctggtga
    43681 cctgacggaa gctaccgcca gatgctttag cattgttttt cgccacttca accacatccg
    43741 ggatcaggtc gtagccttcc ggatgggcca gggtgacatc cataccgaag cgagtcatca
    43801 gaccgatgat gccttgtggt acagagagcg gtttgccata gcttggagag taggcccagg
    43861 tcatggcgat ttttttacct ttcaggtttt ccagtgaacc aaagtgttca cgtaaccacg
    43921 cgaggtcagc cattgactga gtcgggtggt caatatcgca ttgcaggttc actaaagccg
    43981 gacgctgtgg cagtacaccc tgcttgtaac cgtcatcaag tgcagcgcca acttcacgca
    44041 tataggcgtt gcctgcgccc agaaacatat cgtcgcgaat accaatagcg tcggcgcaga
    44101 aggagatcat attggcggtt tcacgcacgg tttcgccgtg agcgatttgt gatttgcctt
    44161 cgtcgagatc ttgttgtgca aggccgagca ggttaagcgc ggaagcataa gagaagcggg
    44221 tacgggtgga gttgtcgcgg aatacggaaa tacctaatcc actattaaag actttggttg
    44281 agatgttttc agcacgcagt gcttttaatg ctgcggcaac gtccagtact tgtttcagtt
    44341 catctggcgt ctgttcccac gttaacaaaa aatctttctc gtgaaggtga gaggtcagcg
    44401 aattgatatc cttaatcagc tcattaacag ttttcatcat aaagtcccgg tagtggaatg
    44461 acaggcaaag tggtttattt cactgtgact tgccaaagtg attggtgttt tcacagctat
    44521 acctgggact aacaataagt gtgccagaat tatccacggg gctgcaaaag actaatttga
    44581 cggataatgt gtggggttga tcacaaattg atgccagctt ccggttgttt gtaaaatacg
    44641 aaaacgtttg gcttttttta tcgcgttatt tatttgctgc taagtatcag aaataagata
    44701 aagggacagg ctatctctct tcagtttgat aaactcaccg ccgtaattct catattgata
    44761 taga
//
LOCUS       DAMZII010000023        44402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZII010000023 DAMZII010000000
VERSION     DAMZII010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.023"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(68..1432)
                     /gene="mnmE"
                     /locus_tag="POA77_001244"
     CDS             complement(68..1432)
                     /gene="mnmE"
                     /locus_tag="POA77_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI6364710.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1538..3184)
                     /gene="yidC"
                     /locus_tag="POA77_001245"
     CDS             complement(1538..3184)
                     /gene="yidC"
                     /locus_tag="POA77_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI6364711.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3187..3444)
                     /gene="yidD"
                     /locus_tag="POA77_001246"
     CDS             complement(3187..3444)
                     /gene="yidD"
                     /locus_tag="POA77_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI6364712.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3408..3767)
                     /gene="rnpA"
                     /locus_tag="POA77_001247"
     CDS             complement(3408..3767)
                     /gene="rnpA"
                     /locus_tag="POA77_001247"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI6364713.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3784..3924)
                     /gene="rpmH"
                     /locus_tag="POA77_001248"
     CDS             complement(3784..3924)
                     /gene="rpmH"
                     /locus_tag="POA77_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI6364714.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4154..4234)
                     /locus_tag="POA77_001249"
     CDS             complement(4154..4234)
                     /locus_tag="POA77_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364715.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4531..5934
                     /gene="dnaA"
                     /locus_tag="POA77_001250"
     CDS             4531..5934
                     /gene="dnaA"
                     /locus_tag="POA77_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI6364716.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5939..7039
                     /gene="dnaN"
                     /locus_tag="POA77_001251"
     CDS             5939..7039
                     /gene="dnaN"
                     /locus_tag="POA77_001251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI6364717.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7039..8112
                     /gene="recF"
                     /locus_tag="POA77_001252"
     CDS             7039..8112
                     /gene="recF"
                     /locus_tag="POA77_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI6364718.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8141..10555
                     /gene="gyrB"
                     /locus_tag="POA77_001253"
     CDS             8141..10555
                     /gene="gyrB"
                     /locus_tag="POA77_001253"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI6364719.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10795..11193
                     /locus_tag="POA77_001254"
     CDS             10795..11193
                     /locus_tag="POA77_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDI6364720.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11308..12120
                     /gene="yidA"
                     /locus_tag="POA77_001255"
     CDS             11308..12120
                     /gene="yidA"
                     /locus_tag="POA77_001255"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDI6364721.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12166..12822)
                     /locus_tag="POA77_001256"
     CDS             complement(12166..12822)
                     /locus_tag="POA77_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364722.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13100..13789
                     /gene="dgoR"
                     /locus_tag="POA77_001257"
     CDS             13100..13789
                     /gene="dgoR"
                     /locus_tag="POA77_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDI6364723.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13786..14664
                     /gene="dgoK"
                     /locus_tag="POA77_001258"
     CDS             13786..14664
                     /gene="dgoK"
                     /locus_tag="POA77_001258"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDI6364724.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14648..15265
                     /gene="dgoA"
                     /locus_tag="POA77_001259"
     CDS             14648..15265
                     /gene="dgoA"
                     /locus_tag="POA77_001259"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDI6364725.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15262..16410
                     /gene="dgoD"
                     /locus_tag="POA77_001260"
     CDS             15262..16410
                     /gene="dgoD"
                     /locus_tag="POA77_001260"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDI6364726.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16485..17822
                     /locus_tag="POA77_001261"
     CDS             16485..17822
                     /locus_tag="POA77_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364727.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17819..18883)
                     /gene="cbrA"
                     /locus_tag="POA77_001262"
     CDS             complement(17819..18883)
                     /gene="cbrA"
                     /locus_tag="POA77_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDI6364728.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            18984..20198
                     /locus_tag="POA77_001263"
     CDS             18984..20198
                     /locus_tag="POA77_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDI6364729.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20200..20532)
                     /locus_tag="POA77_001264"
     CDS             complement(20200..20532)
                     /locus_tag="POA77_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI6364730.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20772..20844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20838..21251
                     /gene="ibpA"
                     /locus_tag="POA77_001265"
     CDS             20838..21251
                     /gene="ibpA"
                     /locus_tag="POA77_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDI6364731.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21363..21791
                     /gene="ibpB"
                     /locus_tag="POA77_001266"
     CDS             21363..21791
                     /gene="ibpB"
                     /locus_tag="POA77_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDI6364732.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21987..23648
                     /locus_tag="POA77_001267"
     CDS             21987..23648
                     /locus_tag="POA77_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDI6364733.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     SGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23645..24361)
                     /locus_tag="POA77_001268"
     CDS             complement(23645..24361)
                     /locus_tag="POA77_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6364734.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24657..26273
                     /locus_tag="POA77_001269"
     CDS             24657..26273
                     /locus_tag="POA77_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6364735.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGLAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26273..27087
                     /locus_tag="POA77_001270"
                     /pseudo
     CDS             26273..27087
                     /locus_tag="POA77_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27084..27977)
                     /locus_tag="POA77_001271"
     CDS             complement(27084..27977)
                     /locus_tag="POA77_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6364736.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAALKNTDQHPTDASPHN"
     gene            28144..29859
                     /locus_tag="POA77_001272"
     CDS             28144..29859
                     /locus_tag="POA77_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDI6364737.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29856..31349
                     /locus_tag="POA77_001273"
     CDS             29856..31349
                     /locus_tag="POA77_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI6364738.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31396..31845)
                     /locus_tag="POA77_001274"
     CDS             complement(31396..31845)
                     /locus_tag="POA77_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364739.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31955..32302
                     /locus_tag="POA77_001275"
     CDS             31955..32302
                     /locus_tag="POA77_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDI6364740.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32292..32654
                     /locus_tag="POA77_001276"
     CDS             32292..32654
                     /locus_tag="POA77_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDI6364741.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32651..33148
                     /locus_tag="POA77_001277"
     CDS             32651..33148
                     /locus_tag="POA77_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI6364742.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33156..34340)
                     /gene="emrD"
                     /locus_tag="POA77_001278"
     CDS             complement(33156..34340)
                     /gene="emrD"
                     /locus_tag="POA77_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDI6364743.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLRLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(34759..34848)
                     /gene="tisB"
                     /locus_tag="POA77_001279"
     CDS             complement(34759..34848)
                     /gene="tisB"
                     /locus_tag="POA77_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDI6364744.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35413..35511
                     /gene="ivbL"
                     /locus_tag="POA77_001280"
     CDS             35413..35511
                     /gene="ivbL"
                     /locus_tag="POA77_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDI6364745.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35617..37305
                     /gene="ilvB"
                     /locus_tag="POA77_001281"
     CDS             35617..37305
                     /gene="ilvB"
                     /locus_tag="POA77_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDI6364746.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37309..37599
                     /gene="ilvN"
                     /locus_tag="POA77_001282"
     CDS             37309..37599
                     /gene="ilvN"
                     /locus_tag="POA77_001282"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI6364747.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37674..38264
                     /gene="uhpA"
                     /locus_tag="POA77_001283"
     CDS             37674..38264
                     /gene="uhpA"
                     /locus_tag="POA77_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDI6364748.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38264..39766
                     /gene="uhpB"
                     /locus_tag="POA77_001284"
     CDS             38264..39766
                     /gene="uhpB"
                     /locus_tag="POA77_001284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDI6364749.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39776..41095
                     /gene="uhpC"
                     /locus_tag="POA77_001285"
     CDS             39776..41095
                     /gene="uhpC"
                     /locus_tag="POA77_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDI6364750.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            41233..42624
                     /gene="uhpT"
                     /locus_tag="POA77_001286"
     CDS             41233..42624
                     /gene="uhpT"
                     /locus_tag="POA77_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI6364751.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42669..>44402)
                     /gene="adeD"
                     /locus_tag="POA77_001287"
     CDS             complement(42669..>44402)
                     /gene="adeD"
                     /locus_tag="POA77_001287"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDI6364752.1"
                     /translation="ISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIK
                     GRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
BASE COUNT        10213 a        11436 c        12062 g        10691 t
ORIGIN      
        1 ataacaggag tgctaacaat gaagcgaagc cgcatctgac agtcagaata cggcttcgta
       61 agcgcggtta cttaccaata cagaagctgg agaaaatccg ccccagcagg tcatcagaag
      121 taaattcccc ggtgatttcg cttaagttct gctgtgccag gcgcaactct tccgccagca
      181 gttcgcccgc ccaggcacct aacagttgcg ctttaccttg ctgcaaatgc tctgccgcct
      241 gttccagcgc ctgtaggtgg cgacggcgcg ccaggaagcc gccttccatg ttggtgtcaa
      301 agcccatgct ctgtttgaga tggttacgca gcacatccac accttcacca gtccttgccg
      361 agagacgaat taacgcgtga ccgttcactt cactcattcc cagcgtttcg ccggtgatat
      421 cggctttatt gcgcaccacg gtgatcggca gtttcgctgg cagacgggcg ataaattccg
      481 gccagatctc tgccggatca acggcgtctg tcgtggtgcc atcgaccata aacagcacgc
      541 ggtcggcctg ttcaatttcc tgccacgcgc gctcgatacc aatacgttcc acttcgtcac
      601 tggcttcacg tagcccagcg gtatcgatga tatgcagcgg cattccgtca atgtggatat
      661 gctcacgcag cacgtcacgt gtggttccgg cgatatcagt tacgattgct gcttcacggc
      721 ccgccagcgc gtttaacagg ctcgatttac cggcgttagg acgtccggca atcaccacct
      781 tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca tcgagatcgg
      841 ccatcacgtc attaagctgg gcttcaattt tgccgtcgga gaggaaatcg atctcttcat
      901 cagggaaatc aattgccgct tcgacgtaaa tgcgcaagtg ggtgagggct tctaccagat
      961 gattaacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg gccgcctgtt
     1021 ccgaactggc gtcgataaga tcggcaatcg cctcggcctg ggctaagtca agtttatcgt
     1081 taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg ggaatggtca
     1141 gaatgcgttt taacagcagg tcgaggatca ccggaccgcc atgaccttgc agttccagca
     1201 catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc tgatcgagca
     1261 cgctgccgtc ggcgtcttta aacggaagat aatcggcgta gcgcggctta ggcagtttac
     1321 ccagcacggt ttcggcaact tcacgggctt tgaagccgga gatgcgcagg atgccaacgc
     1381 cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc atgatgttcc
     1441 tgttgctttg tgtggcggat gcgcggtgct tatccgccct acgaaaagaa aaaaggcggt
     1501 caactgaccg cccttatttt agcgaaaact caccgaatca ggattttttc ttctcgcggc
     1561 tatgcaggcc acgtttttcc agaccacggt aaatcagctg ctgctgaata atggttacca
     1621 ggttgctgac gatatagtac agcaccagac ctgacgggaa ccacaggaag aacacggtga
     1681 agatgaccgg cataaaggtc atgatcttct gctgcatcgg gtcggtcacg gtggtcggcg
     1741 acatcttctg aatgaagaac atcgttacgc ccatcaggat cggcaggatg tagtacgggt
     1801 cctgtgcaga caggtcgtgg atccacagtg caaacggtgc ctgacgcagt tcaacggaac
     1861 ccatcagcat gtagtacaac gccaggaaga ttggcatctg gatcagcagc gggaagcagc
     1921 cgcccagcgg gttaaccttc tcagctttgt acagcgccat catttcctgg ctgatacgct
     1981 gtttgtcatc gcccagacgc tcacgcattg cctgaatctt cggctgcagc atacgcatct
     2041 tcgccatgga ggtgtactgc gctttggtca gcgggtacat gatgccacga acgataaagg
     2101 tgatgatgat aatggagaag ccccagttac ccacaaagct atggatccat ttcagcagtt
     2161 tgaacagcgg ctgagagatg aaccacaacc aaccgtaatc aacggtcaga tccaggtgcg
     2221 gagcaacagc tgccattttg tcctggattt ccgggccaac ccacagggtg ctgttcatcg
     2281 cgccagtctg accaggctga accagtaccg gctgagattt atagccgata gcggcgatgc
     2341 cgttacccag attagcggta tagaagttgt tggtaccgtc gttatgcggg atccacgccg
     2401 tcgcgaaata ctgttgcagc atcgccaccc aaccaccttt cgaagagatg ttcaggtttt
     2461 cgttatcggc aatggtatcg aacttgtatt tctcatactt ctcgtcaggc gtggagtacg
     2521 ccgcgccacg gaaggtgtgc agtgcgaagt tgctgcttcc ggtatcgaga tgcggtggca
     2581 gagtgatgga ttgcttcaac tgaccaaacg aggagatttc cagcggtttc tcgccagcgt
     2641 tctgcacgtt gtagttgacg ttgacagcgt aatcaccacg tttcaggaca aacgttttgg
     2701 taaacgtgtt gcctgccgcg tcggtatacg tcatcggcac ctgcagttcg ttttgacctt
     2761 cagccagcac ataagcgtct ttttcaacgt tatacagcgg acgcgggccg ttagccgggt
     2821 tatccgggcc atcacgaccg gtcagaccgc tctgtgcctg ataaataaac tgcggtgaag
     2881 tttccagcag ctggaacggc tgggtagagt tcagctcttt cgggtaagca ggcagcagag
     2941 cttgctcaac atcaccacca cgggtgttga tggtcagatc aagcacgtcg gtcttaaccg
     3001 agatcagttt cccctggcca ctggccggta cgccctggtc ggcggcgcta cccgctgcgg
     3061 tggtcgttgt ctgcgtggtc tgttgggcct gaggttgcgg gtttttatcc tgctcccagg
     3121 cttgccagat catgaaagac acgaacagca aagcgatgac taaaagattg cgttgcgaat
     3181 ccatcgttag tgttctctgg tatcaaatgg tccgggcggg acgggatcgt caccaccagg
     3241 gtgtaaaggg tggcatttta atacgcgttt caccgtcaac caactgcctt ttatcactcc
     3301 aaacctgcgc aatgcctcaa ttccgtagct tgaacaggtt ggagtgaaac gacaatgcgg
     3361 cccgagtagc ggactaatca ggcgttgata gacccgaatg agggctatca ggacccgcga
     3421 gccaggcgac agtggcggcg ccataatttt tccaacgctt ccgagagagc acggttatcg
     3481 aggtcggcaa cccctttttt cgccaccacc acgaaatcca tagccgggag ttcatgttgg
     3541 cgcagacgga agctttcacg cgtcagacgt ttaatccgat tgcgttcatg ggcgcgtcga
     3601 acgtttttct tggcgactgt aagaccgata cggggatgcc ccagcgaatt caggcggccg
     3661 agaatggtaa tttgcggcgt gccagcccgt tgtggctgct ggaagacgaa tgtgaattga
     3721 ctgggagtta acaagcgtaa ctccctggga aatgcgagct taaccactca ggggttagct
     3781 ttattactta gaaacggtca gacgagcgcg gcctttagca cgacgacgtg ccagaacctg
     3841 acgaccattt ttagtagcca tacgagcacg gaagccgtga gaacggttgc gcttcagtac
     3901 agacggttga aaagtgcgtt tcatggcgat ttctacctaa acttgaataa attcaatggc
     3961 tttattggat atccgccgaa aaatgaaacg atggacaccg aagccatggg tgattaaaga
     4021 ggccggattg taataattgt acactccgga gtcaattctc tttccttatt taccgcgctt
     4081 ttccgcacct tttcgcaggg aaaatgtacg acctcacacc agtgaaaacc agcatggcgc
     4141 gccgggtgga ggattatacg ggctgatggg taaagcgcaa ggatcgtcct ggatctttat
     4201 tagatcgatt aagccaattt ttgtctatgg tcattaaatt ttccaatatg cggcgtaaat
     4261 cgtgcccgcc tcgcggcagg atcgtttaca cttagcgagg tctggaaagt cctgtggata
     4321 aatcgggaaa atctgtgaga aacagaagat ctcttgcgca gtttaggcta tgatccgcgg
     4381 tcccgatcgt tttgcaggat cttgatcggg catataaccg cagacagcgg ttcgtgcgtc
     4441 accctcaagc agggtctttt cgacgtacgt caacaatcat gaatgtttca gccttagtca
     4501 ttatcgactt ttgttcgagt ggagtccgcc gtgtcacttt cgctttggca gcagtgtctt
     4561 gcccgattgc aggatgagtt accagccaca gaattcagta tgtggatacg cccattgcag
     4621 gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt cctcgattgg
     4681 gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat
     4741 gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc acaagcggca
     4801 gtgacgagca acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg
     4861 ccttctacgc gctcaggttg ggataacgtc ccggccccgg cagaaccgac ctatcgttct
     4921 aacgtaaacg tcaaacacac gtttgataac ttcgtcgaag gtaaatctaa ccaactggcg
     4981 cgcgcggcgg ctcgccaggt ggcagataac cctggcggtg cctataaccc gttgttcctt
     5041 tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa cggcattatg
     5101 gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg
     5161 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat
     5221 gcactgctga tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt
     5281 ttccacacct tcaacgccct gctggaaggt aatcaacaga tcattctcac ctcggatcgc
     5341 tatccgaaag agatcaacgg cgttgaggat cgtttgaaat cccgcttcgg ttggggactg
     5401 actgtggcga tcgaaccgcc agagctggaa acccgcgtgg cgatcctgat gaaaaaggcc
     5461 gacgaaaacg acattcgttt gccgggcgaa gtggcgttct ttatcgccaa gcgtctacga
     5521 tctaacgtac gtgagctgga aggggcgctg aaccgcgtca ttgccaatgc caactttacc
     5581 ggacgggcga tcaccatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag
     5641 gaaaaactgg tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa
     5701 gtcgcggatc tcctttccaa gcgtcgatcc cgctcggtgg cgcgtccgcg ccagatggcg
     5761 atggcgctgg cgaaagagct gactaaccac agtctgccgg agattggcga tgcgtttggt
     5821 ggtcgtgacc acacgacggt gcttcatgcc tgccgtaaga tcgagcagtt gcgtgaagag
     5881 agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc gtaaacctat
     5941 gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt
     6001 aggtggtcgt cctacgctac cgattctcgg taatctgctg ttacaggttg ctgacggtac
     6061 gttgtcgctg accggtactg atctcgagat ggaaatggtg gcacgtgttg cgctggttca
     6121 gccacacgag ccaggagcga cgaccgttcc ggcgcgcaaa ttctttgata tctgccgtgg
     6181 tctgcctgaa ggcgcggaaa ttgccgtgca gctggaaggt gaacggatgc tggtacgctc
     6241 cgggcgtagc cgtttttcgc tgtctaccct gccagcggcg gatttcccga acctcgatga
     6301 ctggcagagt gaagtcgaat ttaccctgcc gcaggcaacg atgaagcgtc tgattgaagc
     6361 gacccagttt tctatggcgc atcaggacgt tcgctattac ttaaacggta tgctgtttga
     6421 aaccgaaggt gaagaactgc gcaccgtggc aaccgacggc caccgtctgg cggtctgttc
     6481 aatgccaatt ggtcaatctt tgccaagcca ttcggtgatc gtaccgcgta aaggcgtgat
     6541 tgaactgatg cgtatgctcg acggcggcga caatccgctg cgtgtgcaga ttggcagcaa
     6601 taacattcgt gcccacgttg gcgactttat tttcacctcg aaactggtgg atggtcgctt
     6661 cccggattac cgtcgcgttc tgccgaagaa cccggacaaa catctggaag caggctgcga
     6721 tctgctcaag caggcgtttg ctcgcgcggc gattctctct aacgagaaat tccgcggtgt
     6781 gcgcctgtat gtcagcgaaa accagctgaa aattactgcc aacaacccgg aacaggaaga
     6841 agcggaagag atcctcgacg ttacctatag cggtgcggag atggaaatcg gcttcaacgt
     6901 cagctatgtg ctggatgttc tgaacgcgct gaaatgcgaa aacgtccgca tgatgctgac
     6961 cgattcggtt tccagtgtgc agattgaaga tgccgcatca cagtcggctg cctatgttgt
     7021 catgccaatg agactgtaat gtccctcacc cgcttgttga tccgcgattt ccgcaatatc
     7081 gaaaccgcgg atctcgcttt atctcccggc tttaactttc tggtaggtgc caacggcagt
     7141 ggcaaaacca gcgtgctgga agccatctat acgctcggcc acggtcgggc gtttcgcagt
     7201 ttgcagattg gtcgcgtcat tcgccatgag caggaggcgt ttgttctcca tgggcgatta
     7261 cagggcgaag agcgcgagac ggcgattggc ttaaccaaag acaaacaggg cgacagcaaa
     7321 gtccgcatcg acggtacaga cgggcataag gtcgcggaac tggcgcacct gatgccaatg
     7381 cagttgataa cgccagaagg gtttacttta ctcaacggcg gccccaaata cagaagagca
     7441 ttcctcgact ggggatgctt tcacaacgaa cccggatttt tcaccgcctg gagcaatctc
     7501 aagcgattgc tcaagcagcg caatgcggcg ctgcgccagg tgacacgtta cgaacagcta
     7561 cgcccgtggg ataaagaact gatcccgctg gcggagcaaa tcagcacctg gcgcgcggag
     7621 tatagcgccg gtatcgcggc tgatatggcc gatacttgta agcaatttct ccctgagttt
     7681 tctctgactt tctctttcca gcgcggctgg gagaaagaga cagaatatgc tgaggtgctg
     7741 gaacgtaatt ttgaacgcga tcgccagcta acctacaccg cgcatggccc gcataaagcg
     7801 gacttacgca ttcgcgccga cggtgcgccg gtggaagata ccttatcgcg tgggcagctt
     7861 aagctgttga tgtgcgcctt acgtctggcg caaggagagt tcctcacccg tgaaagcggg
     7921 cggcggtgtc tctacctgat agatgatttt gcctctgagc ttgatgatga gcgtcgcggg
     7981 ctgcttgcca gccgcttaaa agcgacgcaa tcacaggtct ttgtcagcgc gatcagtgct
     8041 gaacacgtta tagacatgtc ggacgaaaat tcgaagatgt ttaccgtgga aaagggtaaa
     8101 ataacggatt aacccaagta taaatgagcg agaaacgttg atgtcgaatt cttatgactc
     8161 ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat
     8221 cggcgacacg gatgacggca ccggtctgca ccacatggta ttcgaggtgg tagataacgc
     8281 tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc acgccgataa
     8341 ctctgtctct gtacaggatg acgggcgcgg cattccgacc ggtattcacc cggaagaggg
     8401 cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa
     8461 ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc
     8521 gcaaaaactg gagctggtta tccagcgcga gggtaaaatt caccgtcaga tctacgaaca
     8581 cggtgtaccg caggctccgc tggcggttac cggcgagact gaaaaaaccg gcaccatggt
     8641 gcgtttctgg cccagcctcg aaaccttcac caatgtgacc gagttcgaat atgaaattct
     8701 ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc gtctgcgcga
     8761 caagcgtgac ggcaaagaag accacttcca ctatgaaggc ggcatcaagg cgttcgttga
     8821 atatctgaac aagaacaaaa cgccgatcca tccgaatatc ttctacttct ccaccgaaaa
     8881 agacggtatt ggcgtcgaag tagcgttgca gtggaacgat ggcttccagg aaaacatcta
     8941 ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag gcttccgtgc
     9001 ggcgatgacc cgtaccctga acgcctacat ggacaaagaa ggctacagca aaaaagccaa
     9061 agtcagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt ccgtaaaagt
     9121 gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg aggtgaaatc
     9181 ggcggtagaa cagcagatga acgaactgct ggcagaatac ctgctggaaa acccaaccga
     9241 cgcgaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg
     9301 cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact
     9361 ggcagactgc caggaacgcg atccggcact ttccgaactg tacctggtgg aaggggactc
     9421 cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa
     9481 gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag atgctctctt cccaggaagt
     9541 ggcgacgctt atcaccgcgc ttggttgtgg tatcggtcgt gacgagtaca acccggacaa
     9601 actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct cgcacattcg
     9661 tacgctgctg ttgaccttct tctatcgtca gatgccggaa atcgttgaac gtggtcacgt
     9721 ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac agtacattaa
     9781 agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggacggcg caacgctgca
     9841 caccaacgcc agcgcaccgg cattggctgg cgaagcgtta gagaaactgg tgtctgagta
     9901 caacgcgacg cagaaaatga tcaaccgcat ggagcgtcgt tatccgaaag caatgctgaa
     9961 agagcttatc tatcagccga cgctgacgga agccgacctc tctgatgagc agaccgttac
    10021 ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg gcagccagtg
    10081 gaagtttgat gtccacacca atgccgaaca aaacctgttt gaaccgattg ttcgcgtgcg
    10141 tacccacggt gtggatactg actatccgct ggatcacgag tttatcaccg gtggcgaata
    10201 tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag atgcgtttat
    10261 cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag caggcgctgg actggctggt
    10321 gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa ggtctgggcg agatgaaccc
    10381 ggaacagctg tgggaaacca ccatggaccc ggaaagccgt cgtatgctgc gcgttaccgt
    10441 taaagatgcg attgctgccg accagttgtt caccacgctg atgggcgacg ccgttgaacc
    10501 gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatatcgata tttaatggcg
    10561 ctaaccatgc gagcgtgcct gatgcgctac gcttatcagg cctacgaaaa tcttgcaatg
    10621 tattgaattt tcatgatttt gtaggccgga taaggcgttc acgccgcatc cggcaagaag
    10681 ctataagaaa agggcggaga tcatctccgc cctttttatt tctgcaatcc gcaattatca
    10741 tcccgcaaat ctatactttt tgggtcaaca cgtaacgctt tacaggtgat tatcatgggg
    10801 ctttttgatg aagttgtcgg tgcctttctg aaaggcgatg cggggaaata tcaggctatt
    10861 ttaagttggg ttgaggagca gggcggcatt caggtgctgc tggaaaaact gcaaagtggc
    10921 ggcttagggg ccattctctc aacctggctg agtaatcaac agggcaatca atcggttagt
    10981 ggcgagcaac tggaatcggc gctcggcaca aatgcggtgt ccgatctcgg gcaaaaactt
    11041 ggcgtggata ccagtacagc ttccagttta ctggcagagc aattgccgaa gattattgat
    11101 gcgctctcac cgcaaggtga agtgtcacca caagccaata acgatctgct ttccgcaggt
    11161 atggaactgc tgaaagggaa actcttccgc taagcaaatg gggagcatgc aacccgcgtt
    11221 gcatccccct tttccctcgg gtgttgtact gatttttgag cggaatcgcg ttagcatggg
    11281 tcaggaacca atctacctgg ggaactcatg gctatcaaac tcattgctat cgatatggat
    11341 ggcacccttc tgctgcccga tcacaccatt tcacccgccg ttaaaaatgc gattgccgca
    11401 gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc gcccttatgc aggcgttcac
    11461 aactacctga aagagctgca tatggaacag ccgggcgact actgcattac ttataacggc
    11521 gcgttggtac agaaggccgc cgatggtagc accgtggcgc aaaccgctct cagctatgac
    11581 gactaccgtt tcctggaaaa actctctcgc gaagtcggtt ctcatttcca cgccctggac
    11641 cgcaccacgc tgtatacagc aaatcgcgat atcagctact acacggtgca tgaatccttc
    11701 gttgccacca ttccgctggt gttctgcgaa gcggagaaaa tggaccccaa tacccagttc
    11761 ctgaaagtga tgatgattga tgaacccgcc atcctcgacc aggctattgc gcgtattccg
    11821 caggaagtga aagagaaata taccgtgctg aaaagtgcgc cgtacttcct cgaaatcctc
    11881 gataaacgcg ttaacaaggg tacgggtgtg aaatcactgg ccgacgtgtt aggtattaaa
    11941 ccggaagaaa tcatggcgat tggcgatcag gaaaacgaca tcgcaatgat tgaatatgca
    12001 ggcgtcggtg tggcgatgga taacgctatt ccttcggtga aagaagtggc gaactttgtc
    12061 accaaatcca accttgaaga tggcgtggcg tttgctattg agaagtatgt gctgaattaa
    12121 tcagtggacg ggcaaacagc ataaacgcgt ttgcccgctt actgattatt ggtttactgc
    12181 atatacaacc agaaattgca gcggtgcagt aatgatgtcc agcgtaacag tcactggcag
    12241 taatgctgcg gaaacaccat ccggaatgcc tctgggtgaa tagtattcat aaaatcgcac
    12301 tttaaatgga tggtagaacg ccatcacctt tgagtaatcc atgtttttat ttttctcatg
    12361 gattgtgcct tttaggtttt ccagtgacct ggtacagtgt tgaacaccac aggcaaaacc
    12421 ataatgcgtt gccagtgcct tttcttcttc gttattccag tagtagtaaa gcgatattgt
    12481 gccggtaaat ttctttttcc ccgcatttaa aacaaatctt gcgtcatcgg caacctgaat
    12541 attgtgccgg ttaagtgctg gatcattgag catcttaacg atgtcatcgg caccatcagt
    12601 gataaggtaa tccagcgatt cccctacaaa aacatagcct tttgttccat tactatcttt
    12661 tgcctgcgat agcccagtga tggtgtctga ctgcgattga tactttcctg aactaccctt
    12721 tgcggtatgg gaaaccagag catacgagat acagcctgaa agcatcagcg ccagtgggaa
    12781 taaaccggca gccttaaaaa atcccttaaa attcaacttc atgattaatc ctttaaacga
    12841 gtagctaagc cgttgaaagc atactcttct ttccctcctg tttagcgcct gatatccctt
    12901 ttcagatttc tgcccgacgc atgtcatttt tttatgcatt gttctttttg tgatctaaat
    12961 tgtagtacaa caatataagt ttgtactaca ttacgcacac ggcaaacgcg aacgtcatca
    13021 cgctggtact acaaagttgc cgcgttatgc atcgatcggg gtaaagtaga gaagaacata
    13081 cagagcacaa ggactctcca tgactctcaa taaaaccgat cgcattgtca ttacgctggg
    13141 taaacagatc gttcacggca aatacgtacc tggctcgcca cttccggctg aggcggagct
    13201 ctgtgaagag tttgcaacct cgcgcaacat catccgtgag gtgttccgtt cgctgatggc
    13261 gaagcggctg attgaaatga aacgttatcg cggggcgttt gtggcaccgc gtaaccagtg
    13321 gaattacctc gacactgacg tactgcaatg ggtgttggaa aacgactacg acccacggct
    13381 tatcagtgcc atgagcgaag tgcgaaatct ggtggaaccg gcgattgccc gttgggcagc
    13441 agagcgcgcg acctccagcg atctggcgca gattgaatcg gcgctgaacg agatgattgc
    13501 caacaatcag gaccgcgaag cgtttaacga agcggatatt cgctaccacg aggcggtgct
    13561 gcagtcggtg cataacccgg tgttacagca acttagcatt gcgatcagtt cgctgcagcg
    13621 ggcggttttt gaacgaacct ggatgggcga tgaggccaac atgccgcaaa cgctccagga
    13681 acataaggcg ctgttcgatg cgatacggca tcaggacggc gatgcggcag agcaggcggc
    13741 gcttaccatg atcgccagct cgacacgaag gttaaaggaa atcacatgac agctcgctac
    13801 atcgcaattg actggggatc gaccaatctt cgcgcctggc tttatcaggg cgaccactgc
    13861 ctggagagca ggcaatcaga agcaggcgtc acgcgcctga acggaaaatc tccggctgcg
    13921 gtgttagcag aagtcacgac cgactggcgt gaagagaata cgccagtggt aatggcagga
    13981 atggtcggca gcaatgtcgg ctggaaagtt gctccgtatt tatctgttcc tgcccgtttt
    14041 tcgtctattg gcgaacaatt aacgtctgtt ggcgacaata tctggattat tcccggatta
    14101 tgcgtctctc atgacgataa ccacaatgtg atgcgcggcg aagaaacaca attgatcggc
    14161 gcgcgaactc tggctccttc ctctctttat gttatgcccg gtacgcattg caaatgggtg
    14221 caggccgata gccagcaaat caacgatttt cgcaccgtga tgaccggtga actacatcat
    14281 ttactgttaa atcactcatt gattggcgca ggtttgccgc cgcaggaaaa ctctgccgat
    14341 gccttcgcgg ctggccttga gcgcggcctt aatgcgcccg ccatattgcc gcagcttttt
    14401 gaagttcgcg cctcgcatgt gctgggaaca cttccccgcg aacaggtcag cgaatttctc
    14461 tctggtttgt tgattggcgc agaggttgcc agtatgcgcg actatgtgac ccatcaacac
    14521 gccatcaccc ttgtcgccgg aacatcgctg accgcgcgct accagcaagc ctttcaggcg
    14581 atgggttgcg acgtgacggc ggtggcgggc gacacggcat ttcaggctgg tataaggagc
    14641 atcgctcatg cagtggcaaa ctaaacttcc gctgatcgcc attttgcgcg gcattaagcc
    14701 cgacgaggcg ctggcgcatg ttggcgcggt gattgacgcc gggttcgacg cggttgaaat
    14761 cccgctgaat tccccacaat gggagcaaag cattcccgcc atcgttgatg cgtatggcga
    14821 caaggcgttg attggcgcag gtacggtact gaaacctgaa caggtcgatg cgctcgccag
    14881 gatgggctgt cagctcatcg ttacgcccaa tatccatagt gaagtgatcc gccgtgcggt
    14941 gggctacggc atgaccgtct gccccggctg cgcgacggcg accgaagcct ttaccgcgct
    15001 cgaagcgggc gcgcaggcgc tgaaaatatt tccgtcatcg gcttttggtc cgcaatacat
    15061 caaagcgtta aaagcggtat tgccatcgga catcgcagtc tttgccgttg gcggcgtgac
    15121 gccagaaaac ctggcgcagt ggatagacgc aggttgtgct ggggcgggct taggcagcga
    15181 tctctatcgc gccgggcagt ccgtagaacg caccgcgcag caggcagcag catttgttaa
    15241 ggcgtatcga gaggcagtgc aatgaaaatc accaaaatta ccacgtatcg tttacctccc
    15301 cgctggatgt tcctgaaaat tgaaaccgat gaaggcgtgg tcggttgggg cgagcccgtg
    15361 attgaaggcc gcgcccgtac ggtggaagcg gcagttcacg agctgggtga ctatttgatt
    15421 ggtcaggatc cttcgcgcat caatgactta tggcaagtga tgtatcgcgc cggattttat
    15481 cgtggcggtc caatcctgat gagcgccatt gccgggatcg accaggcgtt atgggatatc
    15541 aaaggcaaag tgctgaatgc gccggtctgg caactgatgg gcggcctggt tcgcgacaaa
    15601 attaaagcct acagttgggt cggcggcgat cgtccggcgg atgttatcga cggcattaaa
    15661 accctgcgcg aaatcggctt cgataccttc aaactgaacg gttgtgaaga actggggcta
    15721 attgataact cccgcgcggt agatgcggca gtcaacaccg tggcacaaat tcgtgaagct
    15781 tttggcaatc agattgagtt tggtcttgat ttccacggtc gcgtcagcgc gccaatggcg
    15841 aaagtgctga ttaaagaact ggagccgtat cgcccgctgt ttattgaaga gccggtgctg
    15901 gcggaacaag ccgaatacta cccgaaactg gcggcacaaa cgcatattcc actggcggcg
    15961 ggtgagcgca tgttctcacg tttcgatttt aaacgcgtgc tggaggcagg cggtatttcg
    16021 attctgcaac cggatctctc ccatgcaggc ggtattaccg aatgctacaa aattgctgga
    16081 atggcagaag cctatgatgt gacccttgcg ccgcactgtc cgctcggacc gattgcactg
    16141 gcggcttgcc tgcatatcga ctttgtttcc tataacgcgg tacttcagga acaaagtatg
    16201 ggcattcatt acaacaaagg cgcggagtta ctcgactttg tgaaaaacaa agaagacttc
    16261 agtatggttg gcggcttctt taaaccgtta acgaaaccgg gcttaggtgt ggaaatcgac
    16321 gaagctaaag ttattgagtt cagtaaaaat gccccggact ggcgtaatcc gctctggcgt
    16381 catgaagata acagcgtagc agagtggtaa ttcctgccac gtaagcccct catcgggcac
    16441 taaaacagca atacaaaaat ataaccctct gtaaattaca gggcatggtg agcggcttcg
    16501 ctatgcccaa aatctggaga aagctcgcta tggatattcc cgttaatgca gcaaagccgg
    16561 ggcgtcggcg ttatctgacg ctggtgatga tctttattac ggtggtcatt tgttatgttg
    16621 accgcgctaa cctggccgtg gcttccgccc atattcagga agagttcggc attacgaaag
    16681 cggaaatggg ctatgtattt tcggccttcg cctggcttta tacgctatgc cagatccccg
    16741 gcggttggtt tttagatcgc gtaggttctc gcgtgactta ttttattgcg atatttggtt
    16801 ggtcagtggc gactttattc cagggctttg ccacgggatt aatgtcatta attggtctgc
    16861 gtgcgataac cggtattttc gaagcgcccg ctttcccgac caataaccgg atggtgacca
    16921 gctggttccc ggaacatgaa cgcgcttccg ccgttggttt ttatacgtct ggtcagtttg
    16981 tcggtctggc gtttttgact ccgctgctga tctggattca ggagatgttg agctggcact
    17041 gggtgttcat tgtcactggt ggtatcggca ttatctggtc gctgatttgg tttaaggttt
    17101 atcagccgcc gcgcctgacc aaaggtatca gcaaagctga actggattac attcgtgatg
    17161 gcggcggtct ggtggatggt gatgcgccgg tgaagaaaga ggcgcgtcag ccgttaacag
    17221 ccaaagactg gaaactggtg ttccatcgta aactgatcgg cgtttatctt gggcaatttg
    17281 cggtggcttc tacactgtgg tttttcttaa cctggttccc gaactattta acccaggaaa
    17341 aaggaatcac ggcgctgaaa gcaggcttta tgaccacggt gccattcctc gcggcgtttg
    17401 tcggcgtcct gctctctggc tgggtcgcgg atctgctggt acgtaagggc ttttcactgg
    17461 gctttgcgcg taaaacgccg attatctgcg gcttgctgat ctccacctgc attatgggcg
    17521 ctaactacac caacgatccg atgatgatta tgtgcctgat ggcgctggca ttcttcggca
    17581 acggttttgc ttcgattacc tggtcgctgg tctcttctct ggcaccgatg cgcctgattg
    17641 gtttaaccgg cggcgtgttt aacttcgccg gtggtctggg cggcatcacc gttccgctgg
    17701 tggtggggta cctggcgcag ggttacggtt tcgcacctgc actggtttat atctccgccg
    17761 tcgcgttgat tggcgcgctc tcttacatcc tgctggtggg cgatgtgaag cgcgttggct
    17821 aatctttcaa ctgtggaata tgcgccatac cactgcgcat aatccacttt cttaaagccg
    17881 gtgcggtcag gcatcggctt tttactatct tgccgaagag ttttaaacgc agtttgcggg
    17941 tggcgcgcca gtaagcggta ttgagcttct ctggctgctt cagtaacacc gaacgcagaa
    18001 tgtctgcgct atccagcgca tagctaatcc cctccagcga actggcgctg ataaatcctg
    18061 ccgcttcgcc aatcagaaag gcgttgtcct taccgcagac aaaatcctgc cagcgtgagg
    18121 gaaacagcac cgtgcatttt tcgcttttca ccgccttacc aaactggaac tgaaaggcgc
    18181 tcattttctc tttcagcgtc gtgaaacgcg tctgaccgtc tttcattgga taagcaccgc
    18241 caaagataaa ataaccgtct ttgctgatac tccatgaata acagttagtt atcgaattat
    18301 caaagatgca ggagtagaac ggcaccggat gtttctctgc gaaccactgc tggatagcga
    18361 catatttacg gatttgatga tccgggtaga gatgtcgccg caccatcgag tttgcgccat
    18421 cggcaccgac cagatagcgg gcagtaatat gttgctccca gccgtctgca cgaaaaatga
    18481 catgccattt atcatcctca cgccagattt ttcggcacag gctatcgtga taaacctcaa
    18541 cgctggcggg gatcagcgat ttcatccaca agtcgaaagc atggcgatta atattgatat
    18601 agcttcgctg gtagttacgc gtgagcgatg cggcgacgtc gacagttttg acgctgaaaa
    18661 tctgcgggtt ggcgatcaca tcgacaggaa gcgtcagtcc atcgcgaata aaagatcgct
    18721 gcgcgtccgg tgccagcaga ccgccgcaag gtttgctgaa accttcagta ccacactggt
    18781 gctttttatc cagcgcgatc acctgcattt tgcctgctaa ttttcgcgcc aacgccgatc
    18841 ctgccgggcc gaggccaata atcgccacgt cgaaatgttc catcttttca ctcacattca
    18901 tcacgatggc aaaagactat tgtctgaaag tgaagtcctt gtgaagtatg atggcgggaa
    18961 ccgttttgta tcaggaccgc gccatgaaac agataacctt tgctccccgt aatcacctgc
    19021 tcaccaatac caatacctgg acgcccgaca gccagtggct ggtatttgac gtgcgtcctt
    19081 ctggggcgtc gtttaccggc gagaccattg agcgtgtgaa tatccatacc ggcgaggtcg
    19141 aggttatcta tcgcgcgtca cagggcgcac acgtcggcgt ggtgaccgtt catccgaagt
    19201 cagagaaata tgtctttatt cacggccctg aaaatcctga tgaaacatgg cattacgact
    19261 tccatcatcg acgcggagtc attgctgaag gcggcaaggt gagcaatctc gatgcaatgg
    19321 atattaccgc gccgtatacg ccaggagcgc tgcgcggcgg cagccatgtg catgtcttta
    19381 gcccgaacgg tgaaagggtg agctttacct ataacgacca tgtaatgcat gaactcgatc
    19441 cggcgctgga tttgcgaaac gtcggtgttg ctgcgccgtt tggcccggtc aacgtacaaa
    19501 agcagcatcc gcgtgaatac agcggtagcc actggtgcgt gctggtgagt aaaaccacgc
    19561 ccacgccgca gcctggcagc gatgaaatca atcgtgctta tgaagaagga tgggtaggaa
    19621 atcacgcgct ggcgtttatt ggcgacacac tttcgccaaa gggcgagaaa gtgccggagc
    19681 tgtttatcgt tgagttaccg caagatgaag ctagctggaa agcagcaggt gatgcgccgt
    19741 taagtggaac ggaaaccacc ctgcccgcgc caccgcgcgg cgtcgtgcag cgacgtttaa
    19801 cctttaccca ccatcgggct tatccggggt tagtcaacgt cccgcgccac tgggtgcgct
    19861 gtaatccgca gggtacgcaa atcgcgtttt taatgcgtga tgataacggc attgtgcaac
    19921 tgtggcttat ctcgccacag ggcggcgagc cgcgccagtt aacccataac aaaacggata
    19981 ttcagtctgc atttaactgg catccgtcag gagaatggtt gggctttgtg ctggataatc
    20041 gaattgcttg tgcccatgcg caaagcggcg aggttgagta tttaaccgaa aaccacgcca
    20101 atccaccttc tgcggacgcc gtggtcttct cgccggatgg tcagtggctg gcgtggatgg
    20161 aaggcggcca gctgtggatc accgaaactg atcgctaaat cagttatcag gcattctggc
    20221 cggtggaata accgcattgg tggcttgcgc gttggcttcg cttttttcaa cgcgtgagcg
    20281 caccgagctg tctttgcgga acacatccca cggcagtaaa agcgtgtcca tcacagcggt
    20341 aaacggcata tcgagaatcg ccagcgactt agtgccccag ttggtttcat cgtcgccaat
    20401 catggttgcg ctggcgcggg tgccgggata cgttccttct ttccctccgg tatgggacat
    20461 cacgctggag catcctccga gtaatgtcat tccgctgcat atcatgaacg ctaacaacac
    20521 atttcttatc ataataaatt catctgttga tcgtgggtgt tggcctgatg agttatagcg
    20581 atcccttgct gaaaataaca tcatcattac gtcgcactgt ggcggctatc gcactttaac
    20641 gtttcgtgct gccccctcag tctatgcaat agaccataaa ctgcaaaaaa aagtccgctg
    20701 ataaggcttg aaaagttcat ttccagaccc atttttacat cgtagccgat gaggacgcgc
    20761 ctgatgggtg ttctggctac ctgacctgtc cattgtggaa ggtcttacat tctcgctgat
    20821 ttcaggagct attgattatg cgtaactttg atttatcccc gctttaccgt tctgctattg
    20881 gatttgaccg tttgtttaac cacttagaaa acaaccagag ccagagtaat ggcggctacc
    20941 ctccgtataa cgttgaactg gtagacgaaa accattaccg cattgctatc gctgtggctg
    21001 gttttgctga gagcgaactg gaaattaccg cccaggataa tctgctggtg gtgaaaggtg
    21061 ctcacgccga cgaacaaaaa gagcgcacct atctgtacca gggcatcgct gaacgcaact
    21121 ttgaacgcaa attccagtta gctgagaaca ttcatgttcg tggcgctaac ctggtaaatg
    21181 gtttgctgta tatcgatctc gaacgcgtga ttccggaagc gaaaaaaccg cgccgtatcg
    21241 aaatcaacta attccctaag gccgcctggc gcggcctgac atctccatgc tcgccgtcag
    21301 ggagcatatg cgaatcttcg gatttgcagg tacttactcg cttcttagaa ggagaaatga
    21361 ctatgcgtaa cttcgattta tccccactga tgcgtcaatg gatcggtttt gacaaactgg
    21421 ccaacgcact gcaaaacgcc ggtgaaagcc agagcttccc gccgtacaac attgagaaaa
    21481 gcgacgataa ccactaccgc attacccttg cgctggcagg tttccgtcag gaagatttag
    21541 agattcaact ggaaggtacg cgcctgagcg taaaaggcac gccggagcag ccaaaagaag
    21601 agaaaaaatg gctgcatcaa gggcttatga atcagccatt tagcctgagc tttacgctgg
    21661 ctgaaaatat ggaagtctct ggcgcaacct tcgtaaacgg tttactgcat attgatttaa
    21721 ttcgtaatga gcctgaaccc atcgcagcgc agcgtatcgc tatcagcgaa cgtcccgcgt
    21781 taaatagcta actagctatt ctctttgccc cgccattctg gtggggcttt ttttgtcttt
    21841 cttcttcaac aatgcgttaa atttcgcctg tttaagatat ttcggcacgt tctttcccgt
    21901 gataatgtgc gctataaggc aaatctgctt caccgattca gccataatcc gtagcaatta
    21961 aaatgttatc tcaagagaaa tggacgatga gtgatatagc attaacggtc agtattctgg
    22021 ctttggtggc agtcgtcggt ttgtttatcg gcaacgtcaa atttcgcggc ataggattag
    22081 gtattggcgg cgtgctgttt ggtgggatca tcgtcggcca ttttgtttct caggcgggga
    22141 tgacattaag tagcgatatg ctgcatgtta ttcaggaatt tggcctgatc ctgttcgttt
    22201 atactatcgg gattcaggta gggccgggct tctttgcctc attgcgcgtc tccggattac
    22261 gcctcaacct gtttgctgtt ctgatcgtca tcatcggtgg tctggttacc gccatcctgc
    22321 ataaactgtt tgatattcca ctgccggtag tgctggggat tttctccggt gcggttacca
    22381 atacgccagc gctgggggca gggcagcaga ttttgcgcga cctgggtaca ccaatggaaa
    22441 tggtcgatca gatggggatg agttacgcga tggcgtatcc attcggcatt tgcgggattt
    22501 tgttcaccat gtggatgttg cgggttattt tccgcgtcaa tgtcgagaca gaagctcagc
    22561 agcacgagtc ttcacgcacc aatggcggcg cgctgatcaa gactatcaat attcgcgttg
    22621 agaaccctaa cctgcatgat ttagccatta aagatgtacc gattctcaac ggcgacaaaa
    22681 ttatctgctc gcgtctgaaa cgcgaagaaa ccctaaaagt tccttcgcca gataccatta
    22741 ttcaactggg cgatttgctg catctggtgg gtcagccagc ggatttacat aatgcgcaac
    22801 tggtgattgg tcaggaggtc gatacttcgc tgtccacgaa aggcactgat ttgcgcgtcg
    22861 agcgtgtggt ggtcaccaat gaaaacgtgc tcggaaaacg tattcgcgac ctgcacttta
    22921 aagaacgcta tgacgttgtt atctcgcgcc tgaaccgtgc cggggtcgaa ctggtcgcca
    22981 gtggcgatat cagcctgcag ttcggcgata tcctcaatct ggtggggcgt ccgtccgcaa
    23041 ttgatgccgt tgccaatgtg ctggggaatg cgcagcaaaa actgcaacag gttcagatgc
    23101 tgccagtgtt tattggcatc gggcttggcg tattgttagg ttctattccc gtctttgtgc
    23161 ctgggttccc ggccgcgttg aaactggggc tggcgggcgg tccgctgatt atggcgttga
    23221 tcctcgggcg tatcggcagt atcggcaagc tgtactggtt tatgccgcca agtgccaacc
    23281 tcgcgctgcg ggagctgggg atcgtgctgt tcctctcggt cgttggtctg aaatctagtg
    23341 gggattttgt aaataccctg gtcaatggcg aagggctaag ctggattggt tatggtgccc
    23401 tgatcaccgc cgttccgctg attactgttg gcattctggc gcggatgtta gccaaaatga
    23461 attacctgac catgtgcggg atgctggcag gttccatgac cgatcctccg gcgctggcgt
    23521 ttgctaataa tcttcatcca accagcggtg cggcggcgct ctcttacgcc actgtctatc
    23581 cgttagtgat gttcctgcgc attatcaccc cccaattact ggcggtgctc ttctggagta
    23641 tcggttaact cttttccggg tggagtcgcc gtaaatggta ctccacctgg tattcgctgg
    23701 catttctgaa catcactgaa taattcaaaa actccccgct ctcgctatat gacaacgagg
    23761 tgatccgcag cagtggcgtg tgttccgcta ccttcatttg ccgcgccagc agtctatcgg
    23821 cgagcactgg cgtcaggctt tcataattac cgccaatcaa aatcccgcac tcttgttcaa
    23881 tatattcaaa cttcgaccct tccaaatgtt gcagcgaaag attacggaac agttttaccg
    23941 gcatatagct gtcttccagc atcagcggtt tcccttccac aaaacgaacg cgacgggaga
    24001 agtagatctg ctcgttgatt tgaatccgta actggctggc aatggccgga ggcgcaggca
    24061 tgatttcaaa aatcagcacc tggctggtga ccgtttttcc ctgccgtttt aacacctcca
    24121 ccagcccggt caggctggcg gtttgatgca gtacatcttt gcgcaccagg taagtgccgc
    24181 tgccgtggcg gcggaccacc aatccccacg ctaccagcag gtcaatggct ttacggatgg
    24241 tcatccgcga taccgcaaat tcttccgcca actttttttc accgggaaga aggctattta
    24301 gcgtgaaatc tgcggaatta agtcgaattc ttaaccgctc cgcaatgctt ttgtagatca
    24361 tctctagatc tcttttttgc ctgcaagcac gggtaatgcg cccacaagtt ctgcttaatc
    24421 gattgaaaaa acatcttttt tttaaagatg tgttcgatgc tgtgtacctg tgctcacaga
    24481 tgtctacttt ttcgcgaaaa cgtagatctc taccgcccaa cgaaaagcat gaaagcgatc
    24541 acgaatccca ttgggtcgtc atgttctgcc gatcgcatct tcctatcctc gctccaggcc
    24601 tgccgcataa ccaatcaggc ttcctactta cagaattgag aaaagaggat gtggaaatgc
    24661 tcagtcaaat tcaacgcttt ggcggcgcga tgttcacgcc agtgctgctg tttcccttcg
    24721 ccgggattgt ggtgggtctt gccatcttgc tgcaaaaccc gatgtttgtc ggggaatcac
    24781 tgaccgatcc gaacagttta ttcgcgcaaa tcgtacacat tattgaagag ggcggttgga
    24841 cggtattccg taatatgccg ctgatttttg ctgtcggttt acccattggc cttgctaagc
    24901 aagcgcaggg gcgtgcttgt ctggcggtga tggtgagttt cctgacctgg aactatttca
    24961 tcaacgcgat gggaatgacc tggggaagct acttcggcgt cgatttcact caggacgcgg
    25021 tggcaggtag cggtctgaca atgatggccg ggattaaaac cctcgatacc agcattatcg
    25081 gcgcaattat catttccggc attgtgacgg cgctgcataa ccgtctgttc gataaaaaac
    25141 tgccggtttt tctcggcatt ttccagggga cgtcttatgt ggtgattatc gccttcctgg
    25201 tgatgatccc ctgtgcctgg ctgacattgc tcggctggcc aaaagtacaa atggggattg
    25261 aatctctgca agcgttcctg cgttcggcgg gtgcacttgg ggtctgggtt tacaccttcc
    25321 tcgaacgtat tctgatccca accggtttac accacttcat ctacggaccg tttatctttg
    25381 gtctggcagc tgttgaaggc ggcattcaga tgtactgggc gcagcatctg caagagttca
    25441 gtctgagcgc cgagccgctg aaatcgttgt tcccggaagg cggttttgcc ctgcacggta
    25501 actcaaaaat ctttggtgcc gtgggcattt ctttagcgat gtacttcact gccgcaccgg
    25561 aaaatcgggt aaaagtggcg ggcttgctga ttcccgcaac cttaaccgcc atgctggtgg
    25621 gaattaccga accgctggaa tttaccttcc tgttcatttc accgttgctg tttgcggtac
    25681 acgccgtgct ggcggcctca atgtcgaccg taatgtatct ctttggtgtg gtgggcaaca
    25741 tgggcggagg tctgattgac caggttttac cgcaaaactg gatcccgatg ttcagcaacc
    25801 acgcggatat gatgctgacc caaatcgcca ttgggttgtg ctttaccctg ctgtacttcg
    25861 tggttttccg caccctgatt ctgcagttca acatgtgcac gccgggacgt gaagatgcgg
    25921 aagtgaaact ctactcaaaa gccgaataca aagcctcgcg aggccaaacc accgcggcag
    25981 agccaaaaaa agagctggat caggctgccg gtatcctgca agccctgggc ggggtcggca
    26041 atatctccag cattaacaat tgtgcgacgc gtctacgcat tgcactgcat gacatgtcac
    26101 aaacgctgga tgacgaagtc tttaaaaagc tgggagcgca cggcgtcttc cgtagtggcg
    26161 atgccattca ggtgatcatt ggtctgcatg tatcccagct gcgtgaacag ctcgatagct
    26221 taattaattc tcatcaatca gcagaaaatg ttgccattac ggaggcagta taatgaccaa
    26281 attctcagtg gttgtcgcag gcggtggaag cacctttacg ccaggcatcg tgttgatgct
    26341 cctggcgaat caggagcgtt tcccgcttcg tgcactgaaa ttttatgata acgatggcgc
    26401 gcggcaggaa gtgattgccg aagcctgtaa agtcatcctt aaagagaaag cgccggacat
    26461 tgcgtttagt tacaccaccg atcctgaagt ggcgttcagc gacgttgatt ttgtcatggc
    26521 gcacatccgc gtcggcaagt acccgatgcg cgaactggat gaaaaaatcc cactgcgcca
    26581 cggtgttgtt ggtcaggaaa cttgcggacc cggcggaata gcgtacggca tgcgttccat
    26641 tggcggcgtc ctggaactgg tggattatat ggaaaaatat tcaccaaatg cctggatgct
    26701 caactactcc aacccggcag ccattgtcgc agaagccacg cgtcgtctgc gcccgaatgc
    26761 gaaaatcctc aacatctgtg acatgccaat cggtattgaa agccggatgg cgcaaattgt
    26821 tgggctgcaa gatcgcaaac agatgcgcgt gcgctactac ggcctgaacc actggtggtc
    26881 ggcgatatcc cgcagtttca gaaagggtta atgagtcagc aagtggcggt ggaaaaactg
    26941 gtcgtggacg cctgggaaca gcgttcatat cagcacctgt ggcaggcgat tacgttgtcg
    27001 aaaacggtac cgagcgcctc ggtcgccaaa gctattctgg atgagttgct ggaggccaac
    27061 aaagcgtact ggccagagtt acgttaattg tgtggactgg catccgttgg gtgctggtcc
    27121 gtattcttta gcgcggctga cggcgcataa ccaaatttat gtttaaaggc tttgctgaaa
    27181 tgaaaggagt cgaagaagtt gagcgtatca gcgacttcgc caaccgagtt tccttgttgc
    27241 tttaatagcg atagcgccag atccagacgc gcatcgaggt aatattcttt cggcgtcttg
    27301 ccggtatagc gtaaaaacag acgacgcaac caggcttcgc tacaggggat cgtggcagcc
    27361 atatcagcta cgctccagcg ttgttgcaga ctggcatgta aagtggcaat taatttttca
    27421 atttgccgcc gttgtggatc ttttttacca tctgcgtaca taagacaaat ccactgataa
    27481 ataattttcg tcagaaaagc gactgccaga ttatttttta ttgcttctgg tgaagttatt
    27541 aactcagcaa cttcggtgag ttcctgatta taaatttcgc cattataaat aacgctttgc
    27601 tgaccaacgg gaatatccat catactggtg ggggtaaatt ccatccagta ctgttcccag
    27661 actaaacctt cacagtgata agagtgaata tccattggct ttaagaatat aatacagttg
    27721 ccatgcagtg ttatttgttc gccattcttt aaaagaattt tcccacatcc ttgtacggta
    27781 tacaccgcaa cccatgagtt ggcgataagc ggtttcttct tgtcccgcgg ccagatcacc
    27841 cggtaatgct cgtcggcgta ggtatgccag agcgagatca gagaaattcc gctggagatc
    27901 acattttcgt taatcaataa gggaatattg tacggagttt cgttttttac atctgaactt
    27961 tgcaattttc cattcatttt aatatccctc cggcgaatta tgctcacaac ctgaacttaa
    28021 cagaaaacaa tccgttcagg gatacgacgt ttgtatttta agaatctgac tgcctgaccc
    28081 gacgcatttt acctctccct atattcatgc gtccgggaca taaacataaa taagggctat
    28141 gagatgaatt cgttacaaat cttgagtttt gtcggtttta cgctgctggt ggcggtgatc
    28201 acctggtgga aggtccgcaa aacagatacc ggatcgcaac aaggctattt tcttgccgga
    28261 cgttcactaa aagcgccggt tattgccgct tcgttaatgc taaccaacct ttccacggaa
    28321 caactggtcg gcctttccgg gcaggcctac aaaagcggca tgtcggtgat gggctgggaa
    28381 gtgacttcag cggtgacgct gatcttcctc gcgctaatct ttttaccgcg ctatctgaag
    28441 cgcggcattg ccaccatccc cgattttctg gaggaacgtt atgataaaac gacgcgtatt
    28501 atcatcgact tctgcttcct cattgccacc ggcgtctgct ttctgccgat tgttctctac
    28561 tccggcgcgt tggcgctcaa cagcctgttt cacgtcgggg aatcgctaca gatttctcac
    28621 ggtgcggcta tctggctact ggtaattttg cttggtctgg cgggaatttt gtatgcggtg
    28681 atcggcggac tgcgcgcaat ggcagtggcg gactccatca acggtattgg gctggttatc
    28741 ggcgggttga tggtgccggt atttggcctg atcgcgatgg gcaagggcag ctttatgcag
    28801 ggcattgagc aactcaccac cgttcacgcc gagaaattaa actcaatcgg tggcccgacc
    28861 gatcccttgc cgattggcgc ggcatttacc ggtttgattc tggtgaacac cttttactgg
    28921 tgtacaaatc agggcatcgt gcaacgcacg ctggcgtcaa aaagcctggc ggaagggcaa
    28981 aagggggcgc tgttaacggc ggtgctgaaa atgctcgacc cgctggtact ggtgctgcca
    29041 gggttgattg cgtttcatct gtatcaggat ttaccgaaag ccgacatggc ctacccgacg
    29101 ctggtcaata acgttctgcc agtgccaatg gtgggtttct tcggcgcggt gttatttggt
    29161 gcggtgatca gtaccttcaa cggctttctg aatagcgcca gtacgttatt cagtatgggt
    29221 atttaccgtc gcatcattaa ccagaatgcc gagccgcagc agctggtcac cgtcgggcgc
    29281 aaatttggtt tctttatcgc tatcgtttcg gtgctggtcg cgccgtggat cgccaacgcg
    29341 ccgcaggggc tgtatagctg gatgaaacag ctcaacggca tttacaacgt gccgctggtt
    29401 accatcatca ttatgggctt tttcttcccg cgcatcccgg cgctggcggc aaaagtggcg
    29461 atggggattg gcataatcag ctacatcacc atcaactatc tggtgaagtt cgacttccat
    29521 ttcctctatg tgctggcctg tacgttctgc atcaacgtgg tcgtgatgct ggtgatcggt
    29581 tttatcaaac cgcgcgccac gccgttcacc ttcaaagatg cgtttgcggt ggacatgaaa
    29641 ccgtggagaa acgtcaagat cgcgtcaatt ggcatcctgt tcgcgatgat tggcgtctat
    29701 gccgggctgg ctgaattcgg cggctacggt acgcgctggt tagcgatgat cagttatttc
    29761 attgccgccg tagtgattgt ctacctgatt tttgacagct ggcggcatcg tcacgaccca
    29821 gccgtaacct ttactcccga cgcgaaggat agcctatgaa acgccccaat tttctgttca
    29881 tcatgaccga tacccaggcc accaatatgg tcggttgcta tagcggtaaa ccgctgaata
    29941 cgcaaaatat tgatagtctg gcggcggaag gtattcgctt taattccgcc tacacctgtt
    30001 caccggtttg tacgcctgca cgcgccggac tatttaccgg tatctacgct aaccagtccg
    30061 gcccgtggac caacaacgtc gcgccaggca aaaacatctc cactatgggg cgctacttta
    30121 aggatgccgg ctatcacacc tgttacatcg gcaaatggca tctcgacggt catgactatt
    30181 tcggcactgg cgagtgtccg ccggagtggg acgctgatta ctggttcgat ggggcgaact
    30241 accttagcga actgacggaa aaagagatca gcctgtggcg caatggccta aacagcgttg
    30301 aggatttaca ggcgaaccat atcgacgaaa ccttcacctg ggcgcaccgc atcagcaatc
    30361 gggcggtaga ttttctgcaa cagcccgcgc gcgccgagga acccttcctg atggtggttt
    30421 cgtatgatga gccgcatcac ccgttcacct gtccggtgga gtatttagag aaatacgctg
    30481 atttttacta cgatctgggc gagaaagctc aggatgacct ggcgaacaaa ccggaacatc
    30541 accgcttatg ggcgcaggcg atgccatcgc cagtcggtga tgacgggctt tatcaccatc
    30601 cgctctattt tgcctgtaat gactttgttg atgaccaaat cggacgggtc atcaatgcct
    30661 taacgccaga gcaacgtgaa aatacgtggg tcatttatac ttccgatcac ggcgaaatga
    30721 tgggcgcaca taagctgatc agtaaaggag cggcgatgta tgacgacatc acccgtattc
    30781 cgctgatcat ccgttcgccg caaggggagc ggcgacaggt cgatacgcca gtcagtcata
    30841 tcgatttact gccgacaatg atggcgctgg cagatattga aaaaccagag attctgccgg
    30901 gggaaaatat ccttgccgtg aaagagccac gcggtgtaat ggtggaattt aaccgctacg
    30961 agattgagca tgacagcttt ggcggtttta ttccggtgcg ttgctgggtg acggatgact
    31021 ttaaactggt actcaacctc ttcaccagtg atgaacttta cgatcgccgt aatgacccaa
    31081 atgaaatgca taacctgatc gatgatatcc gttttgcaga cgttcgcagc aaaatgcatg
    31141 acgccttatt ggattacatg gacaaaattc gcgatccgtt ccgcagttac caatggagcc
    31201 tgcgtccgtg gcgtaaagat gcactgccgc gctggatggg ggcatttcgt ccacgcccac
    31261 aagatggcta ttcgccggtg gtacgtgact atgacaccgg cctaccgacg caaggagtga
    31321 aagtggaaga gaaaaaacag aagttctgac gataatgccc gctgatgaac acccggcggg
    31381 cattattcag gctgcttaaa ccagtgaacc gccggtcagg aaggaaaaaa tcaggaatat
    31441 cagcaaaatg ctgcacacga tgccgatacc aaaaagcaag gtatggaaag ccagcgtccc
    31501 gccgccaaaa accagcgcgc cgcgaatgcc ccagcgattt tccttacgca taccgccaat
    31561 aaatgcgcaa agtagcgcag caatggccag tgcgataccg ctttgatcga ggattttatc
    31621 gacatctacg tcctcttttt tctccacggt ggtaattttt tcgcctttta ttccggcgag
    31681 aagacctctt ttaatttcag ccgttttgtc cgccacgatg ctttcaaacg ttggcggtgg
    31741 tgcggaaaaa gggccgaatg aaaagtggat aatccccatc attaaagcga tggcaccgaa
    31801 cagcatgccc aatgaactga ttttattttg agctatgata cccatctgac ctccttgtag
    31861 cggatggcga aagcatatca ggaaacgggc ggacgaaatt cttaaaaaaa aaccgctaaa
    31921 ggtagagcgt tagtgaaatt gcaaaggagc aacaatgaaa atttcccgcc ttggagaagc
    31981 gcccgattac cgcttctcgc tggctaatga gcgtactttt ctggcgtgga tccgtaccgc
    32041 actaggattt ctggcggcgg gggttgggct tgatcaactt gcgccagatt tcgccacgcc
    32101 cgtcattcgt gaactgctgg cgttgttgtt gtgtctgttt tccggcgggt tagcgatgta
    32161 tggctacctg cgctggttac gcaatgaaaa agcgatgcgt ctgaaagagg acctgcccta
    32221 caccaacagc ttattaatca ttagcttaat attgatggtc gtggcggtga tcgttatggg
    32281 actggtgttg tatgccggat agccgcaaag ccagacgcat tgccgaccca ggactgcagc
    32341 cggagcgcac atcactggcg tggtttcgta ccatgctggg ctacggcgcg ttgatggcgt
    32401 tggctatcaa acacaactgg caccaggcgg gcatgttatt ctggatttcg attggcattc
    32461 tcgccatcgt ggcgctgatc ctctggcact acacccgtaa tcgcaattta atggatgtca
    32521 cgaatagcga tttttcccaa tttcacgtag tccgtgacaa atttttgatc tccctcgcgg
    32581 tgttatctct cgcaatactg tttgctgtaa cgcatataca tcaacttatc gtatttattg
    32641 agagagtcgc atgacaggaa gtcaggtcat tgatgctgaa gaggacaggc ataaactggt
    32701 tgtagagtac aaggatgctc tgcaacctgc cgatttttat cacaatttca aacagcgggg
    32761 catccgctcc gtacagctca ttccctatct tgaatttgat gaccggggcg atctgacggc
    32821 cgcttcggtg acagcagagc tgtgggggaa atttttaatt gccctgttcg agtgttgggt
    32881 gcgggcagat atcagtcgta tctcgattga gctttttgac gccaccttac aaaaatggtg
    32941 tggcagcgaa aacccgcaac cgcgccgtga ctgccaggcg tgcgactggc atcgcctgtg
    33001 cccgcacgct cgccaggaga cgccagacag cgtgctttgt gcgggctatc aggcgtttta
    33061 ttcttactcc gcaccgcata tgcgggtgat gcgtgatctg ataaagcagc atcgttctcc
    33121 catggagctg atgacgatgc tgcggtgacg tgcgcttaaa cgggctgccc ctgatgcgac
    33181 atccgcgtcg ccagcggcag ccagcacagc acgatcaaca atcccattaa ggtcatcaac
    33241 aaccccaggc tgccctgacc ggtttgcggc aacatcgcag agagcgacgc cagcacgccg
    33301 gaaccaatgt tttgcagacc gccgaccagc gcgccagccg tgcccgccag gaaggggaac
    33361 ggctccatcg cgccgctggt cgccagcgga aacagcatcc cggcaccgaa aaagaacagc
    33421 gcggcgggaa cgagcagcgt ccagacattc atcacgccaa accagtcggg gatccacatc
    33481 agcaagccag ccagcaggca gcagataacc gactgccaca ttaacgtgga gaagcgttta
    33541 ttgggacgtc cggcaaacca tgcgccaaaa aacgctgccg gaatcggcag aataaacaaa
    33601 atactgaccg tcatactgct cagccttaac accgcgccca tcagcacgcc ggagcaggct
    33661 tcaaaggcgg caatcccggc cagaccgcca atcagcatca gcaaataaca gttaaaaccg
    33721 ctgttaccga aaagcgtttt ataactggta agcaggcgcg tgcgcggtgc atcgaccgga
    33781 cgcgtttccg gcatccagcg ggccatactg aaggtcacac cagcacaaag aaccaacaaa
    33841 aagagataac aggcgcgcca gttccacatc gtatccagca gaccgccgat tagcggtgcg
    33901 agcaacggac tgacgagaat ccccatgttt aacaggctgt tagcatggcg caactgtgtc
    33961 cgttcatata aatctcgcgg caaagtacgc gccattacgc cgccaacgcc ggtgcccatc
    34021 ccctgcatcg cgctggcggc aatcaacacc gtcaaactgg aggtcgtgac cgcgaccagc
    34081 gttgccagca taaaaatgga cattccgacg aggatcaccg gtcggcggcc cacgcggtcg
    34141 gaaatcgggc cataaaacag ctgtgagaca ccgtaagtca gcagataagc gcccattacg
    34201 ctctgcaccg ccccttcacg gacgttgaga tcgcgcgcca tatcggcaat agctggaata
    34261 taaatggttt gcgccatctg accgacggcc acgagtaata ccaacatcaa taacaaattg
    34321 acgtttcttt gccttttcat tatcacggat gcttttataa aaataattag aataagtcac
    34381 tacgcattcc cataaatgcg ggagaggaat ctatcacatt gatgaatgtt agccagatat
    34441 acgccaggaa tggagaattg tttagcggca gaatatgtaa acaaaagcgg caataaatgt
    34501 tgccgggagg gattacgtgc gaactgtaac cctctcccca aaggggcgag ggggaaacca
    34561 ccgttccctt tcagaaaatg agctaaacat tgcacgggac ggtcccctcg cccctttggg
    34621 gagagggcta gggtgagggg cacaggcagc gcaggtgcaa acattctgct ggtagaagaa
    34681 gcccccaact ccaacccgca cgctaaatat aaaaggggag cggttacccg ctcccctttg
    34741 gtgcgacttg aatctgaatt acttcaggta tttcagaaca gcatcaagca gttgcagtgc
    34801 tgcaacaatg agtttgagga taagaatggc gatatccacc aggctcatac gcgtctcctg
    34861 tggttcagga gcactgccag ctgacgtacc tttccgctgc ctgttgccag ctgttgtgca
    34921 cgcggtatct taaggagaaa cgttgtgctt gttgacataa cacagtgtgc tcacgcgggg
    34981 ttaaggcgct gacggcacca cccgtttcag ccaggactta gtgcgccggg taacgatgcg
    35041 gcccccgcat aacacattgc gtacagtgat attatagaag tttactgtat aaataaacag
    35101 taatatttgg acaaaacgca aactgtgatc gatactcaac tatgccctaa atacgtcaaa
    35161 attcgtgccg aaattgcgcg ttctgcgcgg aacacgtata ctttcagtgt tgacataata
    35221 cagtgtgctt tgcggttacc agccgcaggc ggctgatgaa acctcgctcc ggcggggttt
    35281 tttgttatct gcaattcagt acaaaacgtg atcagcccct caattttccc ttgctgaaaa
    35341 attttccatt gtctcccctg taaagctgtg cttgtataaa tattgttaaa cacaaaacca
    35401 acaaggtccc caatgactac ttccatgttc aacgcaaaac tactaccaac tgcgctatcc
    35461 gccgcagtgg tcgtcgtgcg tgtggtggtg gtcgtcggca atgcgccgta gggactggaa
    35521 caacacacga ttccaaaacc ccgccggcgc aaaccgggcg gggtttttcg tttaagcacc
    35581 tcccggaaag tcggcccaga agaaaaggac tggagcatgg caagttcggg cacaacatcg
    35641 acgcgtaagc gctttaccgg cgcagaattt atcgttcatt tcctggaaca gcagggcatt
    35701 aagattgtga cgggcattcc gggcggttct atcctgcctg tttacgatgc cttaagccaa
    35761 agcacgcaaa tccgccatat tctggcccgt catgaacagg gcgcgggatt tatcgctcag
    35821 gggatggcgc gcaccgacgg taaaccggcg gtctgtatgg cctgtagcgg accgggtgcg
    35881 actaacctgg tgaccgccat tgccgatgcg cggctggact ccatcccgct gatttgcatc
    35941 accggtcagg ttcccgcctc gatgatcggc accgacgcct tccaggaagt ggacacctac
    36001 ggcatctcta tccccatcac caaacacaac tatctggtca gacatatcga agaactcccg
    36061 caggtcatga gcgatgcctt ccgcattgcg caatcaggcc gcccaggccc ggtgtggata
    36121 gacattccta aggatgtgca aacggcagtt tttgagattg aaacacagcc cgctatggca
    36181 gaaaaagccg ccgcccccgc ctttagcgaa gaaagcattc gtgacgcagc ggcgatgatt
    36241 aacgctgcca aacgcccggt gctttatctg ggcggcggtg tgatcaatgc gcccgcacgg
    36301 gtgcgtgaac tggcggagaa agcgcaactg cctaccacca tgactttaat ggcgctgggc
    36361 atgttgccaa aagcgcatcc gttgtcgctg ggtatgctgg ggatgcacgg cgtgcgcagc
    36421 accaactata ttttgcagga ggcggatttg ttgatagtgc tcggtgcgcg ttttgatgac
    36481 cgggcgattg gcaaaaccga gcagttctgt ccgaatgcca aaattattca tgtcgatatc
    36541 gatcgcgcag agctgggtaa aatcaagcag ccgcatgtgg cgattcaggc ggatgttgat
    36601 gacgtgctgg cgcaattgat cccgcaggtg gaagcgcaac cgcgtgcaga gtggcaccag
    36661 ttggtagcgg atttgcagcg cgaattcccg tgtccaatcc cgaaagcgtg cgatccatta
    36721 agccattacg gcctgatcaa cgccgttgcc gcctgtgtcg atgacaatgc gattatcacc
    36781 accgatgtgg gtcagcatca gatgtggacc gcgcaagctt atccgctcaa tcgcccacgc
    36841 cagtggctga cctccggtgg gctgggcacg atgggttttg gcctgcctgc ggcgattggc
    36901 gcggcgctgg cgaacccgga tcgcaaagtg ttgtgtttct ccggcgacgg cagcctgatg
    36961 atgaatattc aggagatggc gaccgccagt gaaaatcagc tggatgtcaa aatcattctg
    37021 atgaacaacg aagcgctggg gctggtgcat cagcaacaga gtctgttcta cgagcaaggc
    37081 gtttttgccg ccacctatcc gggaaaaatc aactttatgc agattgccgc cggattcggc
    37141 ctcgaaacct gtgatttgaa taacgaagcc gatccgcagg ctgcattgca ggaaatcatc
    37201 aatcgccctg gcccggcgct gatccatgtg cgcattgatg ccgaagaaaa agtttacccg
    37261 atggtgccgc caggtgcggc gaatactgaa atggtggggg aataagccat gcaaaacaca
    37321 actcatgaca acgtgattct ggagctcacc gttcgcaacc atccgggcgt aatgacccac
    37381 gtttgtggcc tttttgcccg ccgcgctttt aacgttgaag gcattctttg tctgccgatt
    37441 caggacagcg acaaaagcca tatctggcta ctggtcaatg acgaccagcg tctggagcag
    37501 atgataagcc aaatcgataa gctggaagat gtggtgaaag tgcagcgtaa tcagtccgat
    37561 ccgactatgt ttaacaagat tgcggtgttt tttcagtaac cgctcaaggc ttgaacaaca
    37621 tcgcgcttat cgttaaggta agcgcgtttt tttttacccg ccaggacaag accatgatca
    37681 ccgttgccct tatagacgat cacctcatcg tccgctccgg ctttgcgcag ctgctggggc
    37741 tggaacctga tttgcaggta gttgccgagt ttggttcggg gcgcgaggcg ctggcggggc
    37801 tgccggggcg cggtgtgcag gtgtgtattt gcgatatctc catgcccgat atctccggtc
    37861 tggaactgct aagccagctg ccgaaaggta tggcgacaat aatgctctct gttcatgaca
    37921 gtccggctct ggttgagcag gcgcttaacg cgggggcgcg cggctttctc tccaaacgct
    37981 gtagccctga cgaactgatt gcggcggtgc atacggttgc tacgggcggc tgttatctga
    38041 cgccggatat tgccattaaa ctggcatccg gtcgtcagga cccgctgacc aaacgtgaac
    38101 gccaggtggc ggaaaaactg gcgcaaggaa tggcggtgaa agagattgcc gccgaactgg
    38161 gcttgtcacc gaaaacggta cacgtccatc gcgccaatct gatggaaaaa ctgggcgtca
    38221 gtaacgacgt agaactggcg cgccgcatgt ttgatggctg gtgatgaaga cgttgttctc
    38281 ccgcttaatt accgttattg cctgcttttt tatcttctct gccgcatggt tttgcctgtg
    38341 gagtatcagc ctgcacctgg ttgagcgccc tgatatggcg gtgctgttat ttccgtttgg
    38401 tctgcgtctg gggctaatgc tgcaatgccc gcgcggatac tggccggtgc tgctgggcgc
    38461 ggagtggctg ctgatttact ggctaacgca ggcggtcggt ttaacccatt ttccgttatt
    38521 gatgatcggt agtttactga cgttactgcc cgtggcgctg atctcgcgct atcgccatca
    38581 gcgtgactgg cgcaccttgc tgttacaggg tgcggcgtta acggcggcgg cgttgttgca
    38641 gtcgctgccc tggctttggc acggcaaaga gtcgtggaat gcgctgttgc tgactttaac
    38701 tggcggcctg acgctggccc cgatatgtct ggtgttctgg cactatctcg ccaataacac
    38761 ctggctgccg ctcggcccgt cactggtttc tcaaccgatc aactggcgcg ggcggcatct
    38821 ggtctggtac ttgctgttgt ttgttattag tctctggctc cagttgggat tgccggacga
    38881 actgtcgcgc tttacgccat tctgcctggc gctgccgatt atcgcgctgg cctggcacta
    38941 cggttggcaa ggggcgctga tcgcgacgtt gatgaacgcc atcgcgctga tcgccagtca
    39001 aacctggcgc gatcatccgg tggatttatt gctctcgttg ctggtgcaaa gtctgacagg
    39061 gttgttactg ggcgctggca tccagcggtt gcgtgaactt aaccagtcgc tgcaaaagga
    39121 actggcgcgc aatcagcatc tggctgaacg gttgctggaa accgaagaga gcgtgcgtcg
    39181 tgatgtggcg cgtgagctgc atgatgatat cggtcagacc atcactgcta ttcgtactca
    39241 ggcgggcatt gttcagcggc tggcggcaga taacgccagc gtgaagcaga gcgggcagct
    39301 catcgaacaa ctatcgctgg gcgtttacga cgcggtgcgc cgtttgttgg gtcggttacg
    39361 tccgcgccag ttggatgatc tcaccctgga gcaggccatc cgctcactga tgcgggaaat
    39421 ggagctggaa gggcgcggta ttgtcagcca tctcgaatgg cgaatcgatg aatcagcatt
    39481 aagtgaaaac cagcgcgtga cgctgtttcg tgtctgccag gaagggctga acaacattgt
    39541 gaaacatgct gatgccagcg cggtcacgct gcaaggctgg cagcaggatg aacggttgat
    39601 gctggttatt gaagacgatg gcagcggttt gccgccgggt tccgggcaac aaggttttgg
    39661 cctcaccgga atgcgcgagc gcgtaacggc gctgggtggc acattacaca tttcctgtct
    39721 gcacggcacg cgtgtcagcg tttctctacc tcaacgctat gtctaaggtt tgatgatgtt
    39781 gccgtttctg aaagcgcctg ccgatgcgcc attaatgact gataaatatg aaattgatgc
    39841 ccgctatcgc tactggcgtc ggcatattct gctgaccatc tggctgggtt atgcgctgtt
    39901 ttacttcacg cggaaaagtt ttaacgccgc cgtaccagaa atccttgcta acggcgtgct
    39961 cagccgtagc gatatcggcc tgttagcgac cctgttttac attacctatg gcgtgtcgaa
    40021 gtttgtctcc ggcattgtca gcgatcgctc aaatgcccgt tattttatgg ggatagggct
    40081 tatcgccacg ggcattatca acattctgtt tggcttctcg acgtcgctat gggcgtttgc
    40141 cgtgctctgg gtgctgaacg cctttttcca gggctggggt tcaccggtgt gtgcgcgtct
    40201 gttaacggcc tggtattcac gtaccgagcg cggcggttgg tgggcattat ggaacacggc
    40261 gcataacgtc ggcggcgcac tcattcccat tgtgatggca gcggctgcgc tgcattacgg
    40321 ctggcgtgcc gggatgatga ttgctggttg tatggcgata gtcgtgggga tttttctctg
    40381 ctggcggcta cgcgatcgcc cgcaggcgtt aggtttaccg gcggtcggtg aatggcgaca
    40441 cgacgcgctg gaaattgctc aacaacaaga aggggcaggg ttgacgcgta aagagatcct
    40501 caccaaatat gtgttgctga atccgtatat ctggctgctt tcgttttgct atgtgctggt
    40561 ctatgtggtc cgggcggcga tcaacgactg gggcaatttg tatatgtccg agacactggg
    40621 cgtcgatctg gtcacggcga atacggcagt gacgatgttt gaactgggcg gatttatcgg
    40681 tgcgctggta gccggttggg gctcggacaa attgtttaac ggcaaccgag ggccgatgaa
    40741 tttgattttc gccgccggaa ttttgctttc agtcggctcc ctgtggctga tgccatttgc
    40801 cagctacgtg atgcaggcaa cctgcttctt caccattggt ttttttgtct ttggcccaca
    40861 gatgttaatc ggtatggcgg cggcagagtg ttcccacaaa gaggcggcag gggcggcgac
    40921 ggggtttgtc ggcttgtttg cttatctggg ggcgtcgctt gctggttggc cgctggcgaa
    40981 agtactcgat acctggcact ggagcggatt ttttgtggtt atctctatcg ccgccgggat
    41041 ttccgcactg ctgttactgc cctttttgaa cgcccagaca ccgcgcgaag cgtgatgcat
    41101 ctcacctttt cacttcatat ccggcaaaac taagaaattt tccaggtttt gcctggacgc
    41161 tatctcaggc ctgatttgct gctgattttt acaatgcatg cctcacgcag gtattcattt
    41221 caggagtaac ccatgctggc tttcttaaac caggttcgca agccgaccct ggaccttccg
    41281 ctcgaagtgc ggcgcaaaat gtggttcaaa ccgttcatgc aatcctacct ggtggtcttt
    41341 atcggctacc tgacgatgta cctgattcgc aagaacttta acatcgcgca gaacgacatg
    41401 atctcgacct acgggttgag catgacgcag ctggggatga tcggcctggg tttctccatc
    41461 acttatggcg tgggtaaaac gctggtttcc tactacgccg acggcaaaaa caccaaacaa
    41521 ttcctgccgt tcatgctgat cctgtctgcc atttgtatgc tgggcttcag tgccagtatg
    41581 ggcagcggct cggttagcct gttcctgatg attgccttct acgccttaag cggctttttc
    41641 cagagtaccg gcggttcgtg cagttactcc accatcacca aatggacgcc gcgtcgtaaa
    41701 cgcgggacat tcctcggttt ctggaatatt tctcacaacc ttggcggtgc aggcgcagca
    41761 ggtgtggcgc tgttcggggc aaattacctg ttcgatggcc atgtcatcgg catgtttatc
    41821 ttcccgtcga ttatcgcgct gattgtcggt tttatcggcc tgcgttacgg cagcgactcc
    41881 ccggaatctt atggcctcgg caaagctgaa gaactgttcg gcgaggagat cagcgaagag
    41941 gacaaagaga cagaatctac cgatatgacc aagtggcaga tctttgttga gtatgtgctg
    42001 aaaaacaaag tgatctggct gctgtgcttc gccaacattt tcctctatgt ggtacgtatt
    42061 ggtatcgacc agtggtcaac cgtatacgcg ttccaggaac tgaaactctc taaagcggtg
    42121 gcgattcagg gctttacgct gtttgaagct ggtgcgctgg tcggtacgct gctgtggggc
    42181 tggctctctg acctggcgaa cggtcgccgt ggcctggtgg cctgcatcgc gctggcgctg
    42241 attatcgcca cgctcggtgt gtatcaacat gccagcaacg aatatatcta tctggcttct
    42301 ctctttgcgt tgggtttcct ggtctttggc ccgcaattgt tgattggtgt ggctgctgtt
    42361 ggctttgtac ctaaaaaagc gattggcgct gccgatggta ttaaaggcac ctttgcttac
    42421 ctgattggtg acagctttgc caagttaggt ctgggaatga ttgccgatgg gacgccggta
    42481 ttcggcctta ccggctgggc aggcaccttc gccgcacttg atatcgccgc gattggttgt
    42541 atctgcctga tggcgatagt ggcggtgatg gaagaacgca aaatccgccg cgagaaaaaa
    42601 attcagcagt tgacagtggc ataaacgtaa ctggtgactt ttgcccggca tgacgccggg
    42661 cttttttatt attccgtgac ttccagcgta gtgaaggcaa acttctcgcc atcaaatagc
    42721 ccctgactgg ttagttttag cgcggggatc actggcagag aaagaaacgc catctgaata
    42781 aacggctcat cgggtaacgg accgcattca cgggcggcgg ctttcaaggc gtcaatttgt
    42841 tccgccagca actgcgccgt gtcggtgctc atcaggccgg caatgggcaa cggcagatga
    42901 ctttggacct ggccgttacg taccacgcac agcccgccgc catcctgaat cacctgattg
    42961 accgccagcg ccatctcttc ggcactgcga ccgatcacca caatattatg gctgtcatgg
    43021 ctgaccgtcg cagccagcgc accttcattc agtccaaagc cgccaagcaa accacaagcc
    43081 ggagccagcc gttgcccgta acgctcaagt acggcaataa agctcacatc atcgcgatca
    43141 aaaccatttt cgctgtagac gctggagtgg gagtgcgtaa tcaattcgtt atggatgacg
    43201 tcaatgaccc gatagcgttt tccgggcgta aattgcaggg caaagtcgct ggcggaaact
    43261 ggctggcggg caatggtgtt gccatatggc ggagcggatt gtgccagtct cgccgactct
    43321 tccgcctgta aggtttgcgc atcaatcggc tcgcctttca ccagtacctg ctgcaccgtg
    43381 accttacgcg catcgctcaa caggacgata tcggcctgct tgccgggtgc cagtaagccg
    43441 aggtgattca gaccaaagtg gcgcgccgtc gaccagctgg cgacgcgata tgccacatgc
    43501 agcggcacat tgtgttgttc gatcaggcgg cgaattaagg catcgatgtg tccttcatgg
    43561 gcgatctccc acgggttacg gtcatcggta cagagcatgc attgcgggct gttaaattcg
    43621 ttgatcaacg gtgccagcgc gttgagattg cgggcagcgg acccttcgcg gatcatcaac
    43681 gacatgccga gttgtaattt ccggcgtcct tcttccagct gataactttc gtggcagttt
    43741 tcaatacccg cagtaatata ggcgttaagt tctttaccac ccaaacccgg gcagtgaccg
    43801 tccagcgtca ggtggcgaaa tgcatccagt ttatcgagca gcgcattctg cccgctaatt
    43861 acgccagggt agtccatcat ttctgcaagg ccggtaacct gcggatggtc ccgccaggcg
    43921 agcatctgtt caagggtaaa actggcaccg ttaacatcgc agccttccag ggcgggtacg
    43981 caagagctga cctgtaagta ctggttttgc cttgcctgtt cggcacagcg ggcaaaccag
    44041 gcgaatccgg cttctcccat cacgttgacg atttcatgag ggtcgcaaat aacggtcgtc
    44101 aggccgcgcg gcagggtagc ggtttcaaaa gtgaccggcg tcatcatgct ggattcaata
    44161 tgcaggtgag catcaataaa ccctggcacc gccgttgcgc cgcgagcatc aatccgctgc
    44221 aaagccggag catcagcgta ttctgcgcca acaccggcaa tgtaacgtcc tttaatcaca
    44281 attgggccgg aaatttctcc gccattgatc aggtcgagaa tagagacatt atcaataata
    44341 taatcggcaa cagcgtcgcc acgggaaacg gctaacaatt cctggtattc agcccggcta
    44401 at
//
LOCUS       DAMZII010000024        43001 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZII010000024 DAMZII010000000
VERSION     DAMZII010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.024"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(699..1175)
                     /locus_tag="POA77_001288"
     CDS             complement(699..1175)
                     /locus_tag="POA77_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDI6364753.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1234..2523)
                     /gene="bioA"
                     /locus_tag="POA77_001289"
     CDS             complement(1234..2523)
                     /gene="bioA"
                     /locus_tag="POA77_001289"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI6364754.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHTPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARNAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2610..3650
                     /gene="bioB"
                     /locus_tag="POA77_001290"
     CDS             2610..3650
                     /gene="bioB"
                     /locus_tag="POA77_001290"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI6364755.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3647..4801
                     /gene="bioF"
                     /locus_tag="POA77_001291"
     CDS             3647..4801
                     /gene="bioF"
                     /locus_tag="POA77_001291"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDI6364756.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4788..5543
                     /gene="bioC"
                     /locus_tag="POA77_001292"
     CDS             4788..5543
                     /gene="bioC"
                     /locus_tag="POA77_001292"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDI6364757.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5536..6213
                     /gene="bioD"
                     /locus_tag="POA77_001293"
     CDS             5536..6213
                     /gene="bioD"
                     /locus_tag="POA77_001293"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI6364758.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6792..8813
                     /gene="uvrB"
                     /locus_tag="POA77_001294"
     CDS             6792..8813
                     /gene="uvrB"
                     /locus_tag="POA77_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDI6364759.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9005..9913)
                     /gene="yvcK"
                     /locus_tag="POA77_001295"
     CDS             complement(9005..9913)
                     /gene="yvcK"
                     /locus_tag="POA77_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDI6364760.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10178..10322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10310..11299
                     /gene="moaA"
                     /locus_tag="POA77_001296"
     CDS             10310..11299
                     /gene="moaA"
                     /locus_tag="POA77_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI6364761.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11321..11833
                     /gene="moaB"
                     /locus_tag="POA77_001297"
     CDS             11321..11833
                     /gene="moaB"
                     /locus_tag="POA77_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDI6364762.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11836..12321
                     /gene="moaC"
                     /locus_tag="POA77_001298"
     CDS             11836..12321
                     /gene="moaC"
                     /locus_tag="POA77_001298"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI6364763.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12314..12559
                     /gene="moaD"
                     /locus_tag="POA77_001299"
     CDS             12314..12559
                     /gene="moaD"
                     /locus_tag="POA77_001299"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDI6364764.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12561..13013
                     /gene="moaE"
                     /locus_tag="POA77_001300"
     CDS             12561..13013
                     /gene="moaE"
                     /locus_tag="POA77_001300"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDI6364765.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13150..13854
                     /locus_tag="POA77_001301"
     CDS             13150..13854
                     /locus_tag="POA77_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6364766.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14057..14704
                     /locus_tag="POA77_001302"
     CDS             14057..14704
                     /locus_tag="POA77_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6364767.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            14774..15487
                     /locus_tag="POA77_001303"
     CDS             14774..15487
                     /locus_tag="POA77_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6364768.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15523..16479)
                     /locus_tag="POA77_001304"
     CDS             complement(15523..16479)
                     /locus_tag="POA77_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDI6364769.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16479..17720)
                     /gene="clsB"
                     /locus_tag="POA77_001305"
     CDS             complement(16479..17720)
                     /gene="clsB"
                     /locus_tag="POA77_001305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDI6364770.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17717..18478)
                     /locus_tag="POA77_001306"
     CDS             complement(17717..18478)
                     /locus_tag="POA77_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI6364771.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18611..19021
                     /locus_tag="POA77_001307"
     CDS             18611..19021
                     /locus_tag="POA77_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDI6364772.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18983..20089)
                     /locus_tag="POA77_001308"
     CDS             complement(18983..20089)
                     /locus_tag="POA77_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6364773.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20100..21233)
                     /locus_tag="POA77_001309"
     CDS             complement(20100..21233)
                     /locus_tag="POA77_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6364774.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21226..22962)
                     /locus_tag="POA77_001310"
     CDS             complement(21226..22962)
                     /locus_tag="POA77_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6364775.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22955..23950)
                     /gene="hlyD"
                     /locus_tag="POA77_001311"
     CDS             complement(22955..23950)
                     /gene="hlyD"
                     /locus_tag="POA77_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDI6364776.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23953..24624)
                     /gene="cecR"
                     /locus_tag="POA77_001312"
     CDS             complement(23953..24624)
                     /gene="cecR"
                     /locus_tag="POA77_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDI6364777.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24853..26217
                     /gene="rhlE"
                     /locus_tag="POA77_001313"
     CDS             24853..26217
                     /gene="rhlE"
                     /locus_tag="POA77_001313"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDI6364778.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(26449..26931)
                     /gene="ybiA"
                     /locus_tag="POA77_001314"
     CDS             complement(26449..26931)
                     /gene="ybiA"
                     /locus_tag="POA77_001314"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDI6364779.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            27051..29201
                     /gene="dinG"
                     /locus_tag="POA77_001315"
     CDS             27051..29201
                     /gene="dinG"
                     /locus_tag="POA77_001315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDI6364780.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29229..30191
                     /gene="ybiB"
                     /locus_tag="POA77_001316"
     CDS             29229..30191
                     /gene="ybiB"
                     /locus_tag="POA77_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDI6364781.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30332..31417
                     /gene="hcxB"
                     /locus_tag="POA77_001317"
     CDS             30332..31417
                     /gene="hcxB"
                     /locus_tag="POA77_001317"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDI6364782.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31646..31906)
                     /locus_tag="POA77_001318"
     CDS             complement(31646..31906)
                     /locus_tag="POA77_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6364783.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32171..32437)
                     /gene="ybiI"
                     /locus_tag="POA77_001319"
     CDS             complement(32171..32437)
                     /gene="ybiI"
                     /locus_tag="POA77_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDI6364784.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32511..33188)
                     /gene="ybiX"
                     /locus_tag="POA77_001320"
     CDS             complement(32511..33188)
                     /gene="ybiX"
                     /locus_tag="POA77_001320"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDI6364785.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRITTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQNALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33230..35512)
                     /locus_tag="POA77_001321"
     CDS             complement(33230..35512)
                     /locus_tag="POA77_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDI6364786.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(35776..36036)
                     /locus_tag="POA77_001322"
     CDS             complement(35776..36036)
                     /locus_tag="POA77_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6364787.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36312..37238
                     /gene="rlmF"
                     /locus_tag="POA77_001323"
     CDS             36312..37238
                     /gene="rlmF"
                     /locus_tag="POA77_001323"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDI6364788.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(37235..39457)
                     /gene="ybiO"
                     /locus_tag="POA77_001324"
     CDS             complement(37235..39457)
                     /gene="ybiO"
                     /locus_tag="POA77_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDI6364789.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVSKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPPPGEPTL
                     "
     gene            complement(39574..40296)
                     /gene="glnQ"
                     /locus_tag="POA77_001325"
     CDS             complement(39574..40296)
                     /gene="glnQ"
                     /locus_tag="POA77_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDI6364790.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(40293..40952)
                     /gene="glnP"
                     /locus_tag="POA77_001326"
     CDS             complement(40293..40952)
                     /gene="glnP"
                     /locus_tag="POA77_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDI6364791.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(41091..41837)
                     /gene="glnH"
                     /locus_tag="POA77_001327"
     CDS             complement(41091..41837)
                     /gene="glnH"
                     /locus_tag="POA77_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDI6364792.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(42241..42744)
                     /gene="dps"
                     /locus_tag="POA77_001328"
                     /gene_synonym="pexB"
     CDS             complement(42241..42744)
                     /gene="dps"
                     /locus_tag="POA77_001328"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDI6364793.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT        10274 a        11033 c        11461 g        10233 t
ORIGIN      
        1 acgataattt cttactggtg atagtatact attattctgg ttaatcgctg tcaaattgat
       61 gattttggtt agtattgcaa taacgaaggg tttgatccgg taagactggc taggcaaggc
      121 tataccaaaa aatcagtgta ggttcagaaa acgatgcacc atacaatcat caataatttc
      181 aaattgtcca ttgtgcataa atcataaaga aaaccgcagc acgtcgtatg caagaacgtg
      241 ctgcggttgg ctggtgaact ttcgatagtg cgagtattga atgatttcca gctgttgccg
      301 attttactat gttttcagta gaacacttag acaaaactga ggcacacaaa tctttgcact
      361 ggattgcaag gctttgtgct tctctggagt gcgacatgtt tgataacaaa aaattagcgc
      421 aagaagacaa aaatcacctt gcgctaatgc tctgtctcag gtcactaata ctatctaagt
      481 agttgattca tagtgactgg atatgttgtg ttttgtagca tcatgtagtc tattttttag
      541 actaaagata ttgtaacaca ttgatattac tggtttttaa tgtttcacgt tcagcttttt
      601 tatactaact tgagcgaaac gggaaggtaa aaagacaaaa agttgttttt aataccttta
      661 agtgatacca gatggcattg cgccatctgg cagagtgatt aactaaacat cgcagtaatc
      721 gaggcgcttg ccagagagtg gaaatgaacg ttaaacccga ccatcgcgcc gctggcacct
      781 tcatcgacat caatacgttc tatatccagc gcgtgaacgg taaaaatgta gcgatgagtt
      841 tcgcctttcg gcggtgctgc gccatcgtac ccggttttac caaagtcggt acgcgtctgc
      901 aaaacgccgt ctggcattgc taccagacca gagccaaacc cttgcggtaa tacgcgggta
      961 tcagcgggta agttaacaac tacccagtgc caccagccgg agccggttgg cgcatccggg
     1021 tcatagcagg tgacgacaaa acttttcgtt cccgcaggaa catcatccca cgccagatgc
     1081 ggtgaaatat tatcgccatc gtaacccatg ccgttaaaga catgacgatg cggcaactta
     1141 tcgccatcgc gcagatcatt actgatgagt ttcatgaacc ctcctttcta gtttgcagaa
     1201 agtgtagcca gaaacccaca cgctgacttc ccgttattgg caaaaaaatg tttcatcctg
     1261 caccgcacgg ttaaccgctg cggtcagacg ctgcaactgt tgcgggagaa taatataggg
     1321 cggcatcagg taaatcagtt tgccaaaagg ccggatccag acaccctgtt cgacaaagaa
     1381 tttttgcagc gccgccatat tcaccggacg agttgtttcg actacgccga ttgcccccag
     1441 cacgcgcaca tcggcaacca tttcggcatt acgggcgggg gcaagttgct cgcgcagctg
     1501 tacttcaata tccgccacct gttgctgcca gtcgccagat tcgagaatcg ccaggctggc
     1561 gtttgcagcc gcgcaggcca gcggattgcc cataaaagtt ggcccgtgca taaagcagcc
     1621 ggcttcgccg ttactgatgg tttctgcaac ctcgcgcgtg gtgagtgtgg cggaaagggt
     1681 cattgtgccg ccggttaagg ctttaccgag gcacaaaatg tccggcgcga tttctgcata
     1741 ttcacaggca aacagtttgc cggtacgacc aaatccggtg gcgatctcgt cggcaatcag
     1801 caagatacct tcgcgatcgc acattttgcg gattcgtttt aaccattccg gatggtacat
     1861 gcgcatcccg cctgcgccct ggacaatcgg ctcaatgatc accgccgcga tttcatgacg
     1921 atgcgccgcc atcaggcggg caaagcccac catatcgcgc tcatcccatt cgccatccat
     1981 gcggctttgc ggggcgggag caaacaggtt ttctggcagg tagcctttcc acagactgtg
     2041 cattgagtta tccggatcgc acaccgacat cgcgccaaag gtatcgccat gataaccatt
     2101 gcggaaggtc agaaaacgct ggcgcgcttc gcctttggct tgccagtact gcaacgccat
     2161 tttcatcgcc acttccaccg ctacggaacc ggagtccgca agaaaaacgc actccagcgg
     2221 ttgcggcgtc atcgccacca gtttgcggca cagctcaatg gctggcgtat gggtgatacc
     2281 gccaaacatc acatgcgaca tggcatcaat ttgcgacttc atcgccgcat taagctgtgg
     2341 gtgattgtag ccgtggatcg ccgcccacca ggacgacata ccgtcaacca ggcgtctgcc
     2401 gtcagacaaa attagctcgc aaccttcggc gctcgccacc ggataaaccg gtagagggga
     2461 ggtcatggat gtgtatgggt gccagatatg gcgttggtca aaggcaagat cgtccgttgt
     2521 cataatcgac ttgtaaacca aattgaaaag atttaggttt acaagtctac accgaattaa
     2581 caacaaaaaa cacgttttgg agaagcccca tggctcaccg cccacgctgg acattgtcgc
     2641 aagtcacaga attatttgaa aaaccgttgc tggatctgct gtttgaagcg cagcaggtgc
     2701 atcgtcagca tttcgatcct cgtcaggtac aggtcagcac gttgctgtcg attaaaaccg
     2761 gagcttgtcc ggaagattgc aaatactgcc cgcaaagctc gcgctacaaa accgggctgg
     2821 aagccgagcg gttgatggaa gttgaacagg tgctggagtc ggcgcgcaaa gcgaaagcgg
     2881 caggatcgac gcgcttctgt atgggcgcgg cgtggaagaa tccccacgaa cgcgatatgc
     2941 cgtacctgga acaaatggtg cagggggtaa aagcgatggg gctggaggcg tgtatgacgc
     3001 tgggcacgtt gagtgaatct caggcgcagc gcctcgcgaa cgccgggctg gattactaca
     3061 accacaacct ggacacctcg ccggagtttt acggcaatat catcaccacc cgcacttatc
     3121 aggaacgcct cgatacgctg gaaaaagtgc gcgatgccgg gatcaaagtc tgttctggcg
     3181 gcattgtggg cttaggcgaa acggtaaaag atcgcgccgg attattgctg caactggcaa
     3241 acctgccgac gccgccggaa agcgtaccaa tcaacatgct ggtgaaggtg aaaggcacgc
     3301 cgcttgccga taacgatgat gtcgatgcct ttgattttat tcgcaccatt gcggtcgcgc
     3361 ggatcatgat gccaacctct tacgtgcgcc tttctgccgg acgcgagcag atgaacgaac
     3421 agactcaggc gatgtgcttt atggcaggcg caaactcgat tttctacggt tgcaaactgc
     3481 taaccacgcc gaatccggaa gaagataaag acctgcaact gtttcgcaaa ctggggctaa
     3541 atccgcagca aactgccgtg ctggcagggg ataacgaaca acagcagcgt ctggagcagg
     3601 cactgatgac cccggacact gacgaatatt acaacgcggc agcactatga gctggcagga
     3661 gaaaatcaac gcggcgctcg atgcgcggcg tgctgccgat gccctgcgtc gccgttatcc
     3721 agtggcgcaa ggagccggac gctggctggt ggcggatgat cgccagtatc tgaacttttc
     3781 cagtaacgat tatttaggtt taagccatca tccgcaaatt atccgtgcct ggcagcaggg
     3841 tgcggagcaa tttggcgtcg gtagcggcgg ttccggtcac gtcagcggtt atagcgttgc
     3901 gcatcaggcg ctggaagaag agctggccga gtggctgggc tattcgcggg cactgctatt
     3961 tatctctggt tttgccgcta atcaggcggt cattgccgcg atgatggcaa aagaggaccg
     4021 cattgtcgct gaccggctta gccatgcctc attgctggaa gctgccagtt taagcccgtc
     4081 gcagcttcgc cgttttgctc ataacgatgt cactcatttg gcgcgattgc ttgcttcccc
     4141 ctgtccgggg cagcaactgg tggtgacaga aggcgtgttc agcatggacg gcgatagtgc
     4201 accactggcg gaaatccagc aggtaacgca acagcacaat ggctggttga tggtcgatga
     4261 tgcccacggc acgggcgtta tcggggagca ggggcgcggc agctgctggc tgcaaaaggt
     4321 aaaaccagaa ttgctggtag tgacttttgg caaaggattt ggcgtcagtg gggctgcggt
     4381 gctttgctca agtacggtgg cggattatct gctgcaattc gcccgccacc ttatctacag
     4441 caccagtatg ccgcccgctc aggcgcaggc attacgtgcg tcactggcgg tcattcgccg
     4501 tgatgagggg gatgcacggc gcgaaaaact ggtgtcactc attgcgcgtt ttcgtgccgg
     4561 agtacaggat ttgccgttta cgctggctga ttcatgcagc gccatccagc cattgatcgt
     4621 cggtgataac agccgtgcgt tacaactggc agaaaaactg cgccagcaag gctgctgggt
     4681 cacggcgatt cgcccgccaa ccgtacccgc tggtactgcg cgactgcgct taacgctaac
     4741 cgcagctcat gaaatgcagg atatcgaccg tctgctggag gtgctgcatg gcaacggtta
     4801 ataaacaagc cattgcagcg gcatttggtc gggcagccgc actctatgag caacacgcag
     4861 atctacagcg ccagagtgct gacgtcttac tggcaatgct cccacagcgt aaatacaccc
     4921 acgtactgga cgcgggttgt ggacctggct ggatgagccg ccactggcgg gaacgtcacg
     4981 cgcaggtgac ggccttagat ctctcgccgc caatgcttgt tcaggcacgc cagaaggatg
     5041 ccgcagacca ttatctggcg ggagatatcg aatccctgcc gttagcgact gcgacgttcg
     5101 atcttgcatg gagcaatctc gcagtgcagt ggtgcggtaa tttatccacg gcactccgcg
     5161 agctgtatcg ggtggtgcgc cccggaggcg tggtggcgtt taccacgctg gtgcagggat
     5221 cgttacccga actgcatcag gcgtggcagg cggtggacga gcgtccgcat gctaatcgct
     5281 ttttaccgcc agatgaaatc gaacagtcgc tgaacggcgt gcattatcaa catcatattc
     5341 agcccatcac actatggttt gatgatgcgc tcagtgccat gcgttcgctg aaaggcatcg
     5401 gtgctacgca tcttcatgaa gggcgcgacc cgcgaatatt aacgcgttcg caattgcagc
     5461 gattgcaact ggcctggccg caacaacagg ggcgatatcc tctgacgtat catctttttt
     5521 tgggagtgat tgctcgtgag taaacgttat tttgtcaccg gaacggatac cgaagtgggg
     5581 aaaaccgtcg ccagttgtgc acttttacaa gccgcgaagg cagtaggtta tcggacggca
     5641 ggttataaac cggtcgcctc tggcagcgaa aagaccccgg aaggcttacg taatagtgac
     5701 gcgctggcgt tacagcacaa cagcagcctg cagctggatt acgcaacggt aaatccttac
     5761 accttcgcag aacccacctc gccgcacatc atcagcgcgc aagagggcag gccgatagaa
     5821 tcattggtaa tgagcgccgg attacgcgcg cttgaacaac aggctgactg ggtgttagtg
     5881 gaaggtgctg gcggctggtt tacgccgctt tctgacactt tcacttttgc agattgggta
     5941 acacaggaac aactgccggt gatactggta gttggtgtga aactcggctg tattaatcac
     6001 gcgatgttga ctgcgcaggc aatacaacac gccggactga ctctggcggg ttgggtggcg
     6061 aacgatgtta cgcctccggg aaaacgtcac gctgaatata tgaccacgct cacccgcatg
     6121 attcccgcgc cgctgctggg agagatcccc tggcttgcag aaaatccaga aaatgcggca
     6181 accggaaagt acataaacct tgccttgttg tagccatcct gtatttggtt aaattgcgag
     6241 cgagatcgcg tcttcgattg actgcaattt aaccaattaa attctaaaat aatcacgaaa
     6301 aaaattttac ttccgcctca tgcggcgaat gtgggaattg cccaggcggc gggggatagg
     6361 ggctggagac agttatccac tattcctgtg gataaccatg tgtattagag ttagaaaaca
     6421 cgaggcaagc gagagaatac gcggcttgca cgcgaattgg cgttaaagac ggctcaaaga
     6481 aatatctttt attttttaac tggttagata aatgcaatgg cagtcactga acaggcatct
     6541 cttgccataa aactgtcatc actcatcttg acaaatgtta aaaaagccgt tgctttgggg
     6601 ataacccggt aaggccggag ttttatctcg ccacagagta aattttgctc atgattgaca
     6661 gcggagttta cgctgtaaca gaaatattat ggtgatgaac tgttttttta tccagtataa
     6721 tttgttggca taattaagta cgacgagtaa aattacatac ctgcccgccc aactccttca
     6781 ggtagcgact catgagtaaa ccgttcaaac tgaattccgc ttttaaacct tctggcgatc
     6841 agccagaggc gattcgacgt ctcgaagagg ggctggaaga tggcctggcg caccagacgt
     6901 tacttggcgt gactggctcc gggaaaacct tcaccattgc caatgtcatt gctgaccttc
     6961 agcgcccaac catggtactt gcgcccaaca aaacgctggc ggcccagctg tatggcgaaa
     7021 tgaaagagtt cttcccggaa aacgcggtgg aatatttcgt ttcctactac gactactatc
     7081 agccggaagc ctatgtaccg agttccgaca ctttcattga gaaagatgcc tcggttaacg
     7141 aacatattga gcagatgcgt ttgtccgcca ccaaagcgat gctggagcgg cgtgatgtgg
     7201 ttgtggtggc gtctgtttcc gcgatttatg gtctgggcga tcctgattta tatctcaaga
     7261 tgatgctcca tctcacggtc ggtatgatta tcgatcagcg cgcgattctg cgccgactgg
     7321 cggagctgca atacgctcgt aatgatcaag cattccagcg tggtactttc cgcgttcgtg
     7381 gcgaggtgat agatatcttc ccggcagaat cggatgacat tgcacttcgc gtggaactgt
     7441 ttgacgagga agtggaacga ttgtcgttat ttgacccgct gaccgggcag attgtttcca
     7501 ctattccacg ttttaccatc tacccgaaaa cgcactacgt cacaccgcgc gagcgcatcg
     7561 tacaggcgat ggaggagatc aaagaagagc tggccgccag acgcaaagtg ctgttggaaa
     7621 acaacaaact gctggaagag cagcggctga cccagcgtac ccagtttgat ctggagatga
     7681 tgaacgagct gggctactgt tcggggattg aaaactactc gcgcttcctc tccggtcgtg
     7741 gaccgggtga gccaccgccg acgctgtttg attacctccc tgctgatggg ctgctggtgg
     7801 tcgatgaatc tcacgtcacc attccacaaa ttggcggcat gtatcgcggt gaccgggcgc
     7861 gtaaagagac actggtggag tacggcttcc gcctgccatc agcgctggat aaccgtccgc
     7921 ttaagtttga agagttcgaa gcattagcgc cgcaaaccat ctatgtttcg gcgacgccgg
     7981 gtaattacga gctggaaaaa tccggcggcg atgtggtgga tcaggtggtg cgtccaaccg
     8041 gattgcttga cccgattatc gaagtgcggc cggtggcgac acaggttgat gatcttcttt
     8101 cggagattcg tcagcgagcg gcaattaacg aacgcgtact ggtcaccaca ctgaccaagc
     8161 ggatggcgga agatcttacc gaatatctcg aagaacatgg cgagcgcgtg cgttatcttc
     8221 actcagatat cgacaccgtc gaacgtatgg agattatccg cgacttgcgt ctgggtgagt
     8281 tcgacgtatt ggtagggatc aacttactgc gtgaaggtct ggatatgccg gaagtttcgc
     8341 tggtggcgat cctcgacgct gacaaagaag gcttcctgcg ttccgaacgt tcgttgatcc
     8401 agaccattgg tcgtgcggca cgtaacgtta acggtaaagc gattctctac ggcgataaga
     8461 tcaccccatc aatggcgaaa gcgattggcg aaaccgaacg tcgccgcgag aaacagcaga
     8521 agtacaacga ggaacacggc attacgccgc aaggcttgaa caagaaagtg gtcgatatcc
     8581 tggcgttggg gcagaacatt gctaaaacca aagcgaaggg cagaggaaaa tcgcgaccga
     8641 ttgttgagcc ggataatgtg ccgatggata tgtcgccgaa agcgttgcag cagaaaatcc
     8701 atgagctgga agggttgatg atgcaacacg cgcagaatct ggagttcgaa gaagcggcgc
     8761 aaattcgtga ccagttgcat cagctgcgtg agctgtttat cgcggcatcg taacaggata
     8821 gcgaagaaga ctgatgacaa acggaaaaca gcctgatgcg ctacgcttat caggcctaca
     8881 ttttctccgc aatatattga atttgcgcgg tttgtaggcc ggataaggcg ttcacgccgc
     8941 atccggcata aacaacgcgc actttgtctt caataggaag ccggaatttt ccttcggatt
     9001 tccgttaacc taaagcctgt aacgcctttt ccagcgcgtt atgtaacaac tggcggtcat
     9061 gacgatacgg aatatcgctg gcctccagta cctcctggat cacaatccgc tctttcaccg
     9121 ccgagacatc cacttttggc ccgacgatga ccgcatcaat gaccttttta ccaacatact
     9181 gctccataat tgccagcttg ctttccagct tcaaattagc cgcaggtaaa ctcaactcac
     9241 gccccagatt gccgatataa accatcggcg ctggcgtgcg gcgtaatgcc tgggcgattt
     9301 ccttcagcag cagaattggc atcaggctgg tataaaaact gccaggccca ataatgatga
     9361 gatccgcttc attgatagcg tgaaccgcct cacgcgttgc gggtacatta ggcgttaaca
     9421 ataactcttg aatcggcgta gttaactggt cgatattgac ctcgccgtaa acttcatgcc
     9481 cctgatcgtc aatcgccatc agatcgacgg gatgctctga cattggaatc aaatgcgtat
     9541 ccactttcag cagattacga attaaattga tggcttccag aggccgcacg ctaaggtgat
     9601 ccagcgcctt taacatcaag tttccgagat tatgaccgga aagttcgcca ttgccgccaa
     9661 aacggtattc aaacatcgcg gaggcgacgc tcggttccgt tatcagctgg ttgaggcagt
     9721 tgcgcatatc gccccaggca atgccgcctt ctgaacggcg aatacgcccc gtcgagccac
     9781 cattatcggt ggtggtgacg atacccgtta aacgagaacc caaagacgaa agtgatgaga
     9841 gaacgcgtcc cagtccatgc cctccgccga gagcaacgac acgatcaaga tcagccagcg
     9901 tacgattgcg catatagttt cctggagtca gattatccgc gctacagtag cgcaaattgc
     9961 ggggaaacag caattaacct gccaatattg aggatacaaa ctattttctt ctacctctaa
    10021 aggacgatgc acgctatgcc tccctgatga tgtatatcaa agtaaaaccg ccattttccc
    10081 ttattctgta gcgaaatagc acgatcatga cgctatatac atgattacat agcgaaagtg
    10141 tggatggtaa aaatctcatt tacacgctag tatcggcata accactaaac actctagcct
    10201 ctgcacctgg gtcaactgat acggtgcttt ggccgtgaca atgctcgtaa agattgccac
    10261 cagggcgaag gaagaaatga cttcgcctcc cgtatctgga aaggtgtaca tggcttcaca
    10321 actgactgat gcatttgcgc gtaagtttta ctacttgcgc ctgtcgatta ccgatgtgtg
    10381 taactttcgt tgcacctact gcctgccgga tggctacaaa ccgagcggcg tcaccaataa
    10441 aggctttctt accgtcgatg aaattcgccg ggttacgcgc gccttcgcca gtctgggcac
    10501 cgaaaaagtg cgcctgacag gcggtgagcc gtctttacgc cgcgacttta ccgatatcat
    10561 cgccgctgtg cgggaaaacg acgctatccg ccagattgcg gtcaccacca atggttaccg
    10621 tctggaacgc gatgtggcga actggcgcga tgcgggactt actggcatta acgtcagtgt
    10681 cgacagtctg gacgcccgcc agtttcacgc tattaccggg caggataaat tcaaccaggt
    10741 catggcgggg attgatgctg catttgaggc cggttttgag aaggtcaaag tcaataccgt
    10801 gctgatgcgt gatgttaatc atcaccagct cgacaccttt ctgaactgga tccagcatcg
    10861 ccctatccag ctgcgtttca tcgaactgat ggaaacgggc gagggcagcg agctcttccg
    10921 taagcatcac atctctggtc aggttctgcg tgacgagcta ctgcgtcgcg gctggatcca
    10981 ccaattacgt caacgcagcg acggtcccgc gcaagtcttt tgccatccag attacgccgg
    11041 agagattggc cttatcatgc cgtatgaaaa agacttctgc gccacttgca accgcctgcg
    11101 cgtttcctcc attggtaaac tccatctctg cctgtttggt gaaggcggcg ttaacctgcg
    11161 cgatctgctg gaagacgata cccagcaaca ggcgctggaa gcgcgtattt cagcggcgct
    11221 gcgggagaag aaacagaccc atttcctgca tcaaaacaac accggtatta cgcaaaactt
    11281 atcgtacatt ggcggctaaa acgtcaaaag gagagatcag atgagtcagg taagcactga
    11341 atttatcccg acccgtattg ctattcttac ggtttctaat cgtcgcggtg aagaagacga
    11401 tacctccggt cactatctgc gcgattcggc gcaagaagcg ggccatcacg ttgtcgataa
    11461 agccattgtg aaagaaaacc gctacgctat tcgcgctcag gtatctgcgt ggatcgccag
    11521 cgacgatgta caagtggtat tgattacggg tggtactggc ctgacggaag gtgatcaggc
    11581 tcccgaagca ttgctgccgt tgttcgaccg tgaagttgaa ggttttggtg aagtgttccg
    11641 gatgttgtct tttgaagaga ttggcacttc cacgttgcaa tctcgtgcgg tagcgggcgt
    11701 cgccaacaaa acgctgattt tcgccatgcc gggttcgacc aaagcgtgcc gtaccgcatg
    11761 ggaaaatatc atcgcgccgc agctggatgc ccgtacgcgt ccgtgtaatt tccatccaca
    11821 tttgaagaaa taagtatgtc gcaactgacc catatcaacg ccgctggcga agcgcacatg
    11881 gtggatgtct ccgccaaagc ggaaaccgtg cgtgaagcgc gggcggaagc ctttgtcacc
    11941 atgcgcagcg agacgctggc gatgattatt gatggtcgcc accacaaagg cgacgtattt
    12001 gccactgcgc gtattgccgg tattcaggcg gcaaaacgca cctgggatct gatcccgctc
    12061 tgtcatccgc tgatgctcag caaagttgaa gtcaatttac aggccgagcc ggagcacaat
    12121 cgggtgcgta tagaaacctt atgccgcctg accgggaaaa ccggtgtcga aatggaagca
    12181 ttaaccgcgg cctccgtggc ggcgctgacc atttatgaca tgtgcaaagc ggtgcaaaaa
    12241 gatatggtga ttggtccggt acgtttgctg gcgaagagcg gcggcaagtc gggtgacttt
    12301 aaggtggaag cggatgatta aagttctttt tttcgcccag gtgcgcgagt tggtgggaac
    12361 agatgcaacc gaagtggctg cggatttccc aactgttgaa gcgttacgcc agcacatggc
    12421 tgcgcagagc gatcgctggg cgctggcgct ggaagatggc aaattactgg ctgccgtcaa
    12481 ccagacgctg gtgagttttg accatccgct gactgacggc gacgaagtag ctttcttccc
    12541 gccggtaacc ggaggttaag atggcagaaa ccaaaattgt tgttggtccg cagccgttca
    12601 gcgtaggaga agagtacccg tggctggcgg agcgtgacga agacggtgcg gtagtcacct
    12661 ttactggtaa ggtgcgcaac cataacctgg gcgacagcgt caaagcatta accctcgaac
    12721 actatccggg gatgactgaa aaagcactgg cagaaattgt tgatgaagcg cgtaaccgct
    12781 ggccgctggg gcgcgtcact gtgattcacc gcatcgggga attatggccg ggcgatgaaa
    12841 tcgtttttgt cggtgtcacc agtgcgcatc gcagcagtgc gtttgaagcc gggcagttta
    12901 ttatggatta tctcaaaacc cgcgcaccgt tctggaagcg cgaagccacg ccggaaggcg
    12961 accgctgggt tgaagcccgg gagagcgatc agcaggcggc aaaacgctgg tagttttttg
    13021 ttagccggat aaggcaccgc tgcgtccggc aatttcaccg ctcagtacat acgtttacgc
    13081 tgcgtcgatg cacagcctca tcattttgca gtatccttaa gatattcctt atatcttcag
    13141 gagatcgtca tggacagatt cccacgttct gattcaatcg tacaaccccg ggctggcttg
    13201 caaacttata tggcgcaagt ctatggctgg atgaccgttg gcttgttgct gaccgcattt
    13261 gttgcctggt atgcggctaa ttccgcggcc gtgatggagc tgttgttcac taaccgtgtc
    13321 tttttaatcg gtctgatcat cgcgcaatta gcattggtta ttgtgttatc agcgatgatt
    13381 caaaagctga gcgcaggtgt aacgacgatg ctctttatgc tttattcggc gctgacgggt
    13441 cttacgcttt ccagtatatt cattgtctat accgctgctt ctatcgccag tactttcgtc
    13501 gttactgccg ggatgttcgg cgcaatgagc ctgtacggtt acaccacgaa gcgcgattta
    13561 agtggctttg gcaatatgct gtttatggcg ttaatcggca ttgtgctggc atcgctggtc
    13621 aacttctggt tgaaaagcga agcattgatg tgggcagtta cctacatcgg cgtgattgtc
    13681 tttgtcggat tgacggcgta tgacacgcag aaactgaaaa atatgggtga gcagattgat
    13741 acccgcgaca cgtcgaacct gcgcaaatat tccattcttg gcgcgttaac cttgtatctg
    13801 gacttcatca acctgttcct gatgttgttg cggatcttcg gcaaccgccg ttaaattctc
    13861 ttcttcctgc tgatcccggc ctggagccgg gatttttttc ggattttata aagactattc
    13921 cgctcattta catgagaact gaatcgcggc aaatgccaga ctatgtccac tacgtaattt
    13981 cagggacaga taacgcattt tgaacagaca atccactgct actcaattac agcaaaaaag
    14041 tgcttcgctc agcaaaatgc tgcttctcat ttggtggtta acagcagctg gagtatgggc
    14101 tacggcaggc tgtgcctgga tatttcagca tttgcattgg gttacgtcac aaaagtctcc
    14161 aggtgctttt gtcttcgctt tggctgcgct gataataggt ggtctctggt taaacaacgc
    14221 cctgaaaaga ctgaatgtat caggcgcatg tctggtctat ctgctaataa tccttcttgt
    14281 tggctttctc ttcgccagcc tttatacctt ttcgagcata gcgacggtaa tgggaattac
    14341 gggagggatg tttgccgcaa tggcgctgat ttgctcttgt agtaataggg ttatcccacc
    14401 agtcagacag ttatattcat atattttctg tggtttgagt attgcttttg ttgttaacct
    14461 gatccttacc agcagttttt ccgtgtggtt agcgagtatt ttgactgtct ttatctgggg
    14521 cattacggct gcctgtgaag caacaacact ggaagatttg atccgtgttg ccgacacctc
    14581 tgaaatttca ggttctcttc gctgtatcgt tcctggggca ataacactct attttagtat
    14641 tttaagcgtc ctctttagaa ttactgtaac agttcttgaa tttattaatg gtttagtgtg
    14701 gtagtgcgaa ttgacgagtt ttattgcgtt aattggaata gataatgata tttaacttaa
    14761 ggatataaaa ataatgaatt cgcgctcagc aaataacata actaatgatt ttcagcacga
    14821 agccaggata ttaaacagca tatggctcat taccgcttta ggtttggtga caaccgcagg
    14881 actggcctgg gtagcgaagt atctcgaaat tacggcaaca aaatatgatt caccgcaaat
    14941 gtatgtcgcc ataggattat tattactttg tatgtatggc ttaagtaagg atatcaacaa
    15001 gataaatgcc gtcatcgcag gcgtaatata tctattttta ctctctttgg tggcgattgt
    15061 cgttgctagt ttagctcctg tacctgccat tatcgtcgtg ttcagcactg cgggcgcgat
    15121 gtttttaatc agtatgctgg ccggtttatt atttaatgtt gatcctggtt ctcaccgttt
    15181 tatcattatg atgacgttga cagggttggc cctggtgatc atcatgaatg ccgcattaat
    15241 gagtgaacga cccgtttggg taataagttg cttaatgatt gtgttatggt caggcattat
    15301 ctcgcatgga cgaaataagc tccttgaatt ggcggggaaa tgccatagtg aagagttgtg
    15361 gagtccggtt cgttgcgctg ttacaggtgc attaacactc tattactatt ttatcggctt
    15421 ctttgggata cttgccgcga tagctataac gcttgtctgg caaaggcata cgcgtttttt
    15481 tcattagtcg taaatgcaga tactaaagaa cttaactaaa cttcacatcg ccgcttcatt
    15541 tttcgcccgt agcttcttcg cctggctttc cagtagcagg taacagatca gcgccagcag
    15601 cagcgggata aagtaataca ggacgcgata agcgagtagg gcggcgataa ttgtgccttt
    15661 ggatgtatgc tccccagcca gtagtgcgat aaacaccgct tccagcacac cgatccccgc
    15721 cggaatatgc acgatgacgc cagcaatact actaaccagt aacacgccca gtacaaagaa
    15781 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15841 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15901 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15961 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    16021 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    16081 ggtggtaata ctgagcgaga aaatccgcgt aatagtgctg cccggtaacc ctagtcggga
    16141 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    16201 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16261 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcccgaccgc
    16321 actaagcagc gcaacgcgat tgtagtcgcg gatgaccttc cagacctctt cccagtccac
    16381 ttttttggcg tagaccacca gcaacacaat caccgcgata aaaaacagcc aggtgaggat
    16441 cttctttgct aagcgccagc gcgggtgtga tttactcatc agggttttac ccccgtgttt
    16501 tcagtttcta cccgatcctg cgtttccatt gtcggttgtg cgggcggatc aacctgcgcc
    16561 agacgtggtg tgtgtgccgg aagccagcca accagcgccg ggaagtggcg taaaaagtgg
    16621 aacgccagca cgcttttggt caggttccac caggtgcgtt tcggcagcat ggtctcatcg
    16681 acctgctgac aatctgcggc aataatgccg ttcagattat cgcgcagcgt ctggttaaaa
    16741 tgacgatcgt ggatgatgac atttgcttcg agattcagtg acaaactgag cggatcgaga
    16801 ttactggacc ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc gtggagcggg
    16861 cggcggcggt actcaaatac ctgaacgccg cctttaacca gatagttata cagcaagcgc
    16921 gcaccgactc tgacaatcgg catatccggt tcgccctgaa tgatcagttt gatccgcacc
    16981 ccgcgccgtg ccgctttacg caaggcgtgt aaaaatcgat agccggggaa gaagtaggcg
    17041 ttggcgataa tgacttctcg ccgcgcctga gtgagcattt tcagatagtg acgttcaata
    17101 tcatcgcgat gttcttcgtt atcgcgccag accagcaata cctgcgcttc tcccggctgg
    17161 cggttctctt ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct ctgtccaggc
    17221 aggttttcca gctcaaactg gagaatatct tcgacaatcg gcccttcaag gcgtaccgcg
    17281 taatcctgtt tagcctctgg accgtagctg gacatatgct cggcggagta attcagcccg
    17341 ccaataaagg cgatacgcgc gtcgatcacc acaattttgc gatgcatccg gcgaaacaca
    17401 ttggtgcgca taccaaaaag gcgagggcgg ggatcgtagt agcgaaatac cacgccagct
    17461 gccgtcagtt cattgacaaa ctcatcgctg agatccggcg aaccgtagcc atccagcaag
    17521 acttccgctt taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag ctgtttgccg
    17581 acgtcatcct caaaccagat aaacgtttca agaatgatgc gttcttgtgc ctcgccaatc
    17641 gccttaaaca ccgcgggata atattgctcg ccgttttcca gcaactggat cttattgcct
    17701 tcgcgccagc tacatttcat aaatgaatct ccgcacttaa aggggcatga tcagaaaggt
    17761 gtcgccatgt ccgcagcggc aacgcggttg gcgcgctggc gctggcattt ttgacgtaga
    17821 tcctgtccag tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg cgtccgtggg
    17881 cgcgggtaaa aatctcatcc agtccggcct gcacttttaa cggatgatta gctttttgcc
    17941 gccagtcatt gaaatcaccc gccaccaata ccggttcgcc gtccggtagc tcattcaccc
    18001 attcggcgag catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc agatgtacgc
    18061 acatcacatg aatcgctttt ccggtcatcg gtggcacaat gcggcagtag agcacgccgc
    18121 gcttttccgc accatcgacc gaaacatcgc gattctcata atgttcaatg ggataacgcg
    18181 acagtacggc gttgccgtga tgcccttccg ggtatacggc attgcgaccg taggcaaaat
    18241 cgctccacat cgtgtcggcg agaaactcgt agtgcgaggt atcgggccag ttttccacat
    18301 gcagcggatg aacttcgtgc gcgcccatca cttcctgcag gcaaacaata tcggcgctga
    18361 cggtacgcac cgcgtcgcga agttccggca aaatgaagcg tcggttaaac gcggtaaaac
    18421 ctttgtgaat gttgatggtg agcaccttga acgaaaattg ttgtgtttga tcgggcataa
    18481 ttttcctgtc tttgcctctt atctcattga aatagtgtag tcggcgtcac aaaaaggtgc
    18541 ggtcttacgg aattttccgt aaagttcggt actctgagta agtagagata aattcttcag
    18601 gagagaagcc atgaagtggc aacaacgtgt tcgtgtcgca actggtctaa gttgctggca
    18661 gattatgttg catttactgg tagtggcgct gctggtggtg ggctggatga gtaagactct
    18721 ggttcacgtc ggcgtgggat tatgcgcact gtattgtgtc acggtagtga tgatgttggt
    18781 gtttcagcgc caccccgagc aacgctggcg tgaggtggca gacgtgctgg aagagctgac
    18841 cacgacctgg tattttggcg cagcgctgat tgtgctgtgg ctgttgtccc gtgtactgga
    18901 aaacaacttt ttgctggcaa ttgcagggct ggcaatcctt gccggcccgg cggtagtgtc
    18961 tttgctggcg aaagataaga agttacatca ccttacgtct aaacatcgcg tacgccgctg
    19021 accctgtcgt ggccgttatc accagtagcg gccacaaact attccacaca atatccagac
    19081 tcgcatcctt caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata gggttaatcc
    19141 acgtcaggtt ttgcagccat actggcatgt tttccaccgg agaaacgtaa ccggaaagga
    19201 gaatggcggg catcataaaa acaaacacgc cgataaacgc ctgctgttgt gtcgagcaga
    19261 gtgatgaaat caacagaccg aatcccacca gcgataaacc gtaaatcacc atcgtaaagt
    19321 agaacagcgc cagcgatccg gcgaagggga tttgatacgc ccagatacca atcgccagca
    19381 caatggtggc ctggaaggtg gcgacaatta acgccggtac ggctttgccg atgaagatct
    19441 gccaggtggt gagcggcgaa accagtagct gatcgagcgt accttgttca cgttcgcggg
    19501 cgacggaaag tgaagtgacg atcattacgc cgatagtggt gatcatggcg atcagcgacg
    19561 gcaccacaaa ccatttgtag tcaagattcg ggttatacca gttgcgtacc accagctcgc
    19621 tgttgttagg tttcggtttt ccttccagca gctcctgctg ataatttttg acgatctgtt
    19681 gcaggtagtt ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg atcaactgca
    19741 acggcgcggt ctggaaggta tccagtttgc gcgagaagtc agccgggaaa cgcaccagta
    19801 gtaacgcctt ttgtgtgtcg atggttgggc ggatctcctg tgggcttttc agcagcagca
    19861 catgagtaaa ggcgctggcg cgggcaaaac gttgggtcag ctccaccgaa tgctcgccgt
    19921 tatcttcatc gtagatggcg atggtggcgt tagtcacttc cagcgtggcg gcgaacggga
    19981 acagaatcac ctgaataagc acaggtaaaa tcagaatcgc gcgggtttgc ggttcgcgca
    20041 gcagcgactg caactctttg cggattaacg tccataagcg atgaaacatg ctcttctccc
    20101 taatccagcc gacgtttggt tttcagccac gtcaggccga taaacatcac cgccgaagcg
    20161 atcaaaaaca gcacgtttac caccagcacc actggaatat tcccggcgag gaacaggctt
    20221 tgcagggtgc tgacgaaata acgagcggga ataatgtacg tcaccgcgcg gatcaccgcg
    20281 ggcatactgt cgatctgaaa aataaagccg gaaagcataa tcgacggcag aaaagcggcg
    20341 ttcagggcga cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat cagcagcccc
    20401 atccccaggg tactgagtaa aaacaggctg gagataaaaa acagaatcag cagcgacccg
    20461 cgatacggca cgccgagaat aaacactgac accagcatac acagcaacat cgccagcatc
    20521 ccgagaaagt aataagggat cagcttacac agcagcagtt ccgtgcgggt aatctccgta
    20581 gagagcagag cctccatggt gccgcgttcc cattctcgcg ccaccaccag cgaggtgaga
    20641 atcgcgccga tgaccgtcat gataatggtc accgcaccgg ggataatgaa gtgctggcta
    20701 atcgccgccg ggttaaacca gtagcgggtt tgtacatcaa taagcggttc aaaagtctgc
    20761 ccgttgtcct ccgctcgctg catttgccag atctgccaga tcccttcgac atacccctgt
    20821 acaaagttag cggtattcgg ctcactgccg tcggtgatca cctgaatcgg tgcggtggcg
    20881 ttggcgcgct ccatctgttc cgcaaaatcc accggaataa ccaccagacc gcgaattttc
    20941 cccgcctgca ttttggcgat cagttcctga cggttatcgc tgatggtggc gtcgatgtag
    21001 ggcgaaccgg tcatggtgtg ggtgaaatcc agcgccgctt cgctacgctg ttccagtaaa
    21061 atcccgaccc gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat aaacagcagc
    21121 aacagcggga tcactaccgc aatcagccag ctactcggat cgcgaacgat ctgccgcgtc
    21181 tctttgacgc acagcgcccg cacgcgacgc caggaaagga tcgggttact cattgctatg
    21241 ctccttatcc cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg gatcgggttg
    21301 ctcatcgtta gccgactgtg ctttcaaatc gtccggcgtg ccgctggcga ttaatttccc
    21361 gcggtacacc aggccgatgc ggtcgcaata ttccgcttca tccataaagt gggtggtgac
    21421 catcaccgtg acgcctttct ctaccatgct gttgatgtgc agccaaaatt cacggcgggt
    21481 gaggggatca acgccggacg tcggttcgtc gagaaacaga atgtccggtt catgcatcag
    21541 cgagcaggcc agtgccagcc gctgtttaaa acctaatggc agttcatcgg tggcgtggga
    21601 ggcgatactt ttcaggccga acgcctcgct catacgggag attttttcgt tctgcgcccg
    21661 accgcgtaag ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg tcaggttacc
    21721 gtagagcgaa aacttttgcg ccatatagcc gagatgctgg cgcgctttac cagaactctc
    21781 tttcagatcc atccccagca ccagcgcctg gccggaagtc ggcaccagca aaccgcacat
    21841 catcttaaag gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa aaatctcccc
    21901 acgtttaacg gcaaagttga cgtgatcggt ggcggcaaaa tcaccaaatt tcttggtcag
    21961 ttctttcgct tcgatcaccg tctcgccggg tgtgccttct accgtatgca atattgcacc
    22021 cagcggcgat tctgaggttc cggcaccacc cagcaaatca ataaatgcat cttcaaaacg
    22081 cggcgtagtt tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa tatcgtctgg
    22141 tgtggcctct tttttgagga tcagacgtac cgatttcccc tgaatcatgc cgtcgctgac
    22201 ctgcggcagt ttcaatgccc gttgcaacag tttgcggttg ccctcgtgtg gactggtcat
    22261 cagaaagctg cgtccggcca tggtttgtgt cagggctttt ggttctccct gatacagcaa
    22321 ctcgccttcg ttcatcagta acacgtcacg gcactgctcg gcttcgtcga gatacgaggt
    22381 actccagagg attaacatcc cttcgcccgc cagctcatgc accatctgcc acagttcgcg
    22441 ccgtgagata gggtcaacgc cgacgccggg ttcatcgagc agcaacactt tcggttcgcc
    22501 caccagggta caggccaggc cgagtttttg tttcatccca ccggagagct tgcccgccag
    22561 gcgtccggta aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag tttgcttacg
    22621 tgcctcgccg gtgacgctgc gcaaatctgc gtaaagattg aggttctcca tcaccgtcag
    22681 atcttcatac agaccaaatt tctgcggcat atagccgagc acggcgtgca gcgcgccgtc
    22741 gtttttgatt ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt tcagtaatcc
    22801 cgccagcatc cgcattagcg tggttttacc tgcaccatcc ggccccacca gccccgtcac
    22861 ataaccggcg tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca tgcccggaaa
    22921 gcgtttttcc aggccgttca gcgtgataac ggcatcattc atgtcctgcc tcgttaccga
    22981 attgcaccgt caccggcatt ccctggcgta acgcatcatc ggcgtcggtc accacaatac
    23041 gcaggcgata gacgaggtcg gtacgcagat ccggtgtttc gacggttttc ggggtaaatt
    23101 cagcagtcgg cgaaacgaaa ccaatctgcc cgtgataggg cttgtccggg cgaccatcgg
    23161 tataaagcag cactttgcgc cccggctggg cctggtcaag attacgttcg tcaacatagg
    23221 cacgtaccca caccggacgc gttagtgaaa cggtaaacac cgtgccacct tcattgagga
    23281 ccgtgcctgg ctccaccgcg cgcgttaaca gcgtgccatc agacggggct atcaacgttg
    23341 agtcctgcaa attcaactcc gcctgcgcca gttgcgcctg cgcctgttcg aggctggctt
    23401 tcgcctgggc gatgtcctgt tcacggttac cggaacggta ctgacgcaat ttatcctgtg
    23461 ctgatttcag cgttgcctgc gcctggtcgc gcgaggagcg ggcattttcc aggtcgtttg
    23521 ccgaaatggt gcggcttttc cacaaccctt gctggcggtt atagaagttc tgcgcatagt
    23581 cataggcggc ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct tcatcgcgat
    23641 acccggcaag catcaggtca tactgcgcct gtgccaccga aacacccgct ttcgcctgca
    23701 tcagggcaat ctcatacggc ttgtgatcca gttcgcccag cacctggccc gctttgatag
    23761 catcaccttc gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa ctaagattca
    23821 ccgtacgaat atccacgttg ccatacagcg tcaggccgtt atcctggcgg ctttgatacc
    23881 accagtagcc tccggcaacc acggcggcaa gtaccactac cgccaatccg atcacgacag
    23941 gttttttcat cactacaaac tcctttgcga taatccttgc aaaatcaggt cgatatgaca
    24001 ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg aacgcggtcc agccggtacg
    24061 taacagaatt gtttctttgc caagacggaa cgccagaatc tcaccaatca acgcatgagt
    24121 atggaggatc atgcgggtat cgttggcgtc gcagccggtc caggcggcaa tcaggcgtgt
    24181 caggtggctg tgtagcgggc taatcacctg ttcgtgcacc aggtgatagg ctgccgtggg
    24241 agagagctgc tcacgggaga taaacttgct gaggttgacg gtgtcatcct gggtgagcag
    24301 cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg atggctgcgc gatcaggctg
    24361 tggttgtgcg aacaagcgtt cggcctcttc ggcgtgcgga cggaactgct cgccaataaa
    24421 atcggcaatc cactgggcgc aggcgaggta caaatcttct ttcgaaccga agtagtaggt
    24481 gatggcagcg atattctgcc cggcctgggc ggctatctcg cgggtggtgg cgttcattcc
    24541 atattcacca aactgcgcca gtgcggcagc aatcagctgt tttttcgcct gttcaccctt
    24601 gattgtcatg gcaggattat tcatcgcaca gtccattctt aatcaaatga ttgattaaga
    24661 ttatgactcc ataggggagt tgtccagtat ggctaagaat tttagctacg ccagtcacag
    24721 ggataattta tgcgctgcgt cacaaaaact gctacactcc gctccctcat gacattgtgg
    24781 tttttgtcat tttccttttc agtatctccc tgaaaactac accggtaacg gtcggggcgg
    24841 ttcggagtag ttatgtcttt cgattctttg ggtttaagcc ctgatatcct gcgcgccgtt
    24901 gccgagcagg gttaccgtga acccacccct attcagcagc aggcgatccc tgcggtgctg
    24961 gaaggccgcg acctgatggc tagcgcccag accggtaccg gtaaaacagc gggctttacg
    25021 ctgccgctgt tgcaacacct gatcactcgc cagccgcacg ccaaagggcg tcgtccggta
    25081 cgtgcgctca ttcttacccc gacccgtgaa ctggctgcgc agattggcga aaacgtccgt
    25141 gattacagca aatatttgaa cattcgttcg ctggtggtgt ttggcggtgt cagcattaac
    25201 ccgcaaatga tgaagctgcg cggcggcgtt gatgttctgg tggcaacacc gggacgtttg
    25261 ctggacctgg aacatcaaaa tgcagtgaag ctggatcagg ttgaaatcct cgtcctcgat
    25321 gaagctgacc gcatgctcga catgggcttt atccacgata tccgtcgcgt gttaacaaaa
    25381 ctgccagcga aacgccagaa cctgctgttc tctgcgacct tctctgacga tattaaagcc
    25441 ctggcggaaa aactgttgca caacccgctg gaaatcgaag tggcacgccg caataccgcg
    25501 tctgatcagg tgacccagca cgttcacttt gtcgataaga aacgcaaacg cgaattgctg
    25561 tcgcacatga ttggtaaagg gaactggcag caggtgctgg tgtttacccg taccaaacac
    25621 ggcgctaacc atctggctga gcagctcaat aaagatggca tccgtagtgc ggcgatccac
    25681 gggaataaat cgcaaggtgc gcgtacccgt gcgctggctg attttaaatc gggtgacatt
    25741 cgcgtactgg tggcgaccga tatcgctgcg cgcggtctgg atatcgaaga gttgccgcac
    25801 gtcgtcaact atgaactgcc aaacgtaccg gaagattatg tccaccgtat cgggcgtacc
    25861 ggtcgtgcgg ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga acacaaactg
    25921 ctgcgtgata tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat tccgggctat
    25981 gagccggacc cgtcaatcaa agccgaaccg atccagaacg gtcgccagca acgtggcggc
    26041 ggcggtcgtg ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg ggaaggtggc
    26101 gcaaaatctg caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg cgatgccaaa
    26161 ccggcaggcg aacaacaacg tcgccgccgt ccgcgtaaac ctaccgctgc gcagtaatct
    26221 tttatgccgg gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc
    26281 tacgcttatc aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc
    26341 ggataaggcg tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga
    26401 acacgggaaa aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat
    26461 agccaattgt tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt
    26521 accaccgtct ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt
    26581 tgccagcaag agcgcacgca gttctggatg ctgttcgaat ttagcgcgaa gtgctttccg
    26641 catcacctgt tctttgaccg actcccagtt tttacgcaga ggcttagaac gatcgcgccc
    26701 catgcgtgcg gcgaccatag gggaagaaac ccgacggatc tcttcgcggt atttttcatc
    26761 aaggaatttt tgtgcctgaa aatagtgttc tgaggtgggc caggttttcc cgtcaacctt
    26821 gatgggccag gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt
    26881 tatgatggtg tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca
    26941 ttatgttggt ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa
    27001 ccgaaaaatg ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa
    27061 ccgccgcgct taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg
    27121 actttattcc ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg
    27181 gagaagaagg gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct
    27241 atttgatccc cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg
    27301 ccaacgtggc gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca
    27361 ttcccgatct taaattcact gccgcttttg ggcgtgggcg ctacgtttgc ccgcgtaatc
    27421 tgacggcgct cgccagcact gagcccacgc aacaggatct gctggcgttt cttgacgacg
    27481 aactgacgcc aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg
    27541 acacttataa atgggatggt ctgcgcgatc atactgatat cgctatagat gacgatctct
    27601 ggcgtcgttt aagtaccgac aaagccagct gcctcaaccg caactgttac tactatcgtg
    27661 aatgcccgtt ttttgttgct cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc
    27721 atgcgctggt gatggcggcg atggaaagcg aagccgtact acctgacccg aaaaatctgc
    27781 tgctggtgct ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga
    27841 gtgccgaaat caccgcgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg
    27901 ctacctgcat ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt
    27961 tgaatgcgca ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc
    28021 tctacatgcc tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg
    28081 aagtgctgga gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg
    28141 agttatttct taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc
    28201 ggttgatttt gcagatgaac cgcgcgttgg ggatgttcga ggcgcaaagc aaactctggc
    28261 ggctggcgtc tctggcgcaa tcttccggtg caccggtgac caaatgggcg acgcgggaag
    28321 agcgcgaagg gcagctgcat ctctggtttc actgcgtggg aatacgtgtc agcgatcagc
    28381 tggaaaggct gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt
    28441 cgctgaacag tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc
    28501 gttttgtggc gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc
    28561 ggatgcgcgt tgagccttcc atcgacaacg aagagcagca tattgccgaa atggcggcct
    28621 ttttccgtaa gcaggtggag agcaaaaaac atctcggtat gttggtactg tttgccagcg
    28681 gacgggcgat gcagcgcttt ctcgactatg tgacggattt acgcctgatg ctgctggtgc
    28741 agggcgatca gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg
    28801 agcgtagtgt gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc
    28861 tgctcagcca ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga
    28921 tcaccgaagg ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc
    28981 cgagcgcctc gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct
    29041 ggggcgaagt ggttatctac gacaaacgct tgctgaccaa aaattatggc aagcgactac
    29101 tggatgcatt accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa
    29161 agaaagaaaa aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg
    29221 agtcgtaaat ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc
    29281 gcgatttaga ccgggatact gcgcgcggtc tgtatgccca tatgctcaat ggtgaagtcc
    29341 ctgaccttga gttgggcggc gtattgattg cgctacgtat caaaggggaa ggggaagcag
    29401 agatgctcgg cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag
    29461 gcaagccgat gccgattgtc atccccagtt acaacggcgc gcgtaaacag gccaacctga
    29521 cgccgttgtt ggcgattctc ctgcacaaac tcggttttcc tgtggtggtt cacggggtta
    29581 gcgaagatcc aacccgcgtg ctgactgaaa ccatttttga attgatgggt attacgccaa
    29641 cgttgcacgg cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgccag
    29701 tcggcgcgtt ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc
    29761 gtaacagtgc acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcgctgc
    29821 gtctttccag cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata
    29881 tcggcgggcg ggcgctacta atgcatggta ctgaaggtga agtgtatgct aatccgcagc
    29941 gctgcccgca gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg
    30001 accctgctgg tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga
    30061 ttgagcgttg ccttgctggc agcgaaccga ttcccgaatc gctgaaaatc cagatggctt
    30121 gctgcctggt ggctacgggt gaagcggcaa ctatcagcga cggcctggcg cgcgttaatc
    30181 aggcatttta attcttttct cagccggatg agccatgtca tccggctttt accccaccgc
    30241 aatatgaaat tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac
    30301 aacaaacaca taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca
    30361 gacgctgcac agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc
    30421 gaaattagtg gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat
    30481 tggcatgatc ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca
    30541 tgccaaaatc gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg
    30601 tcaggtcgcg gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat
    30661 tgctgccgtg gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca
    30721 gtgtgcagcg gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt
    30781 cgcaccgttc cacggtcgcg acagccgctt tggcaccaat ccgttctgtg tggttttccc
    30841 tcgtaaagat aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa
    30901 aacccgcgtc gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa
    30961 cggcgtgccg acgaccaatc cggcggtaat gcaggagtcg ccgttgggtt cgctgttgac
    31021 ctttgccgaa cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct
    31081 ttccggcggt aaaacgacgc atcaggaaac gttacaaacc agtcccgatg ccattcttaa
    31141 ctgcatgacc actatcatca tcaacccgga actcttcggc gcgccggatt gtagcgcgca
    31201 gaccgaagcc tttgccgagt gggtgaaagc ctcgccgcat gatgatgata agccgatttt
    31261 gctaccgggc gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt
    31321 ggatgcggga agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga
    31381 gacgttgcag gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga
    31441 cgggcaaaca agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa
    31501 cagacaggga aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg
    31561 attttgtagg cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc
    31621 gtaaacgcct tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat
    31681 tgttgttggt ggcggaagtg atgctgtatc cactggcacc ggctgccgcg gctttctcag
    31741 ccagtttcgc ttccagggca tcgagggtgg atgcgccatc ggcagaaacc acgccgattt
    31801 tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc ggcaaacacg ccaaatgaca
    31861 gagttgaaag agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc
    31921 aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac
    31981 gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca
    32041 tactgataaa ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac
    32101 agcagcgtta aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg
    32161 ccagttgtag ttaacgtaac tggctgtctt tcgaacctct gcgattatat cctgtatacg
    32221 ctggtttttg taaatctttc tcctgctgac aatgaatgca taagcgcacg ccaggaatgg
    32281 cttcccgacg ggcttgcggg atgggggcac cgcactcttc acattcatcc aggctttcgc
    32341 cacgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt
    32401 cgttgacggc gtcatcgtta gcccaaccgg atgccatatc gtcctcccca tatcaatgct
    32461 tgtacagtta agtgtagcta atccagggac gaacctgggc agttcaagca tcagatctcc
    32521 gaccattccc gcagcagatt atgataaaga ttaagcagcg acaggatctc ttcactttcg
    32581 ccgtagcggc ttttcagcga ctgaatattg ttgtccagtt caaacagcat ggcgcgcttt
    32641 ttatcatcac ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt
    32701 acgggtgtca cgcaatgcag gctgctggag ggatacaaca cgagatcgcc tgccgggagt
    32761 tttacccgat gttgtccgaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt
    32821 ggatcgctta aaaacagcgt ggcagaaagg tcagtacgca tccagccgtt ttgtggatgg
    32881 ctgcgtactg cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac
    32941 agcggcgtgg aaagggtacg cggcaaggcc gcggcaaaga acaaagcatt ttggttaacc
    33001 gcgttcagca cctcattttg cagggcggcg tataacgtgc tgcgggtgtc gacctgttga
    33061 ttgttcttaa cttgcgcacc ttgtgcgccg gtggtgatgc gtccatccac ccattcggct
    33121 tgttccagtt gttcgcgaaa ccgggcgacg tcctgtggcg ataacacgcc gggaatgtgg
    33181 tacatcatac aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca
    33241 tattggctgt gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt
    33301 tgattgaggc gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat
    33361 tgcgattaac ccgatacccc agtttggcat cggcgaccca gtaaccttcg gtaaacgctg
    33421 gcgttcccac cgcgccgtct gaacctttat gcatactgcc gatatagcgt gcgcccgcgc
    33481 caacagagat atcatctgtt gcctgatact ggctccataa ggtgaaggcg tgctccgggg
    33541 tatacggcag cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt
    33601 tttgctgggt atagccgcca atcacctgcc acgcgggagt gatattcccg gccacggata
    33661 tctcatagcc ttcgacacgt ttcttaccgt attgcgagta agttccgtca tcattttgct
    33721 caacttcatt ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca
    33781 gaacctgcca tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc
    33841 ggttggcact gttaccgctg ccagactgcg caagggcgaa gttgttgccg cccggaggct
    33901 gctgggaaac ggcatagtta atatagacat taccgttttc cgtcaggtga tacagcgccc
    33961 cggctttcca gttcaccaga ttgcccgact tggcggtgtc gacggtggtg accggagaac
    34021 cttttgccac accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg
    34081 cactgtcata ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac
    34141 gggtgatttg cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt
    34201 ttgcgccgtt gcgcgtcagg ccgccgggat gaatgctgct gtcaggatga taaatattta
    34261 ccgcgggtaa cgtcaccgga ttaacgccgt agttagtctg cgtttcacgg gtaaattcca
    34321 cgccggtact gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct
    34381 ggttggtgag aattttatta ctcacatctt tggtattcgc cgtgcgtgac caggtccagc
    34441 tattcacatc gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca
    34501 ggtaatcctg ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga
    34561 tgtcgtgctc aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg
    34621 tgccgtaaaa gttatgagta tcaacttttc cggaatgatt cagagccgct gttcccgcag
    34681 atggggcaga ataacccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct
    34741 gggtgacatg cagataatta agatacaaac gattcgctgt accaaggcca aaagcgatag
    34801 aaggggcgac gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgcgtttttt
    34861 cgcccatcac attcaggcgc accgcagtgg tatcaccaat gacctgattg acgtccagcg
    34921 tgccgcggcg gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc
    34981 gcggctgctt gctgatcata ttgatcgagc ccgtcggcgc gctgcgcccg tagtcggtgc
    35041 cggaaggccc tttaatcact tcgacctgct cagtattgaa ggtgtcgcgc gagacgctgc
    35101 cgatatcgcg aatgccatca atataaatac tgttagaggt atcagcaccg cgcatataaa
    35161 tggcgtcgcc agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccgggga
    35221 cgtttttgag cgcgtcggta aggttggttg cgccctgatc tttaatcact tgttcagaaa
    35281 tcaccgtcat cgtgcgggta gtatccgcta ccggacgcga gaatttcgga tcggcagatt
    35341 gttgtggcgc ataaagcgaa ggcgttgatg cttcgacaac cagcgtgtca tccgcgttag
    35401 tttgcccttc ggcggcgagt gcctgagcca caggcgtgat gccaatacaa agaccggcaa
    35461 agaacgtgag cgaatgaaat tgtctggcag ggaaattgcg attgttttcc attttgcagg
    35521 tgactttttt tatatgttga aattttcact gccacgagaa agcctctctg cggatgcgat
    35581 gccactgtta atctcgtgat agtgttcgtg caagtgataa taattttgat aagcattatc
    35641 acttggcgcg attttctatc acccattgcg cgaaaaagaa atacatttat ttacattgcg
    35701 ttcatatttg atgttttttg attacaaaac aggcgaaaac tgcatccaga ggcgaatgca
    35761 gttacgttgg tggggttatt tgtagatggt tgcagtaccg aacatctggt catttccgcc
    35821 tgcggaatta atgacataac ctttcgcgcc ttgctcgcgc gctttttctg ccagtttatc
    35881 ctccagatcg cttaggtttg atgcgccagt tgccgaaacg gtaccggcgg gccgtagctg
    35941 accgctgtcc aggttactca tcggttgcgc ggcataagcc gtgagagaga ttccgctcag
    36001 gacggtggca atcagtagtg tgaggcactt tttcataatg acatcctcat aaatacaacg
    36061 ggtagtgcct gataagtgta ggtcgcctgg gcgatgaaaa tgacgcaaaa aattgacaga
    36121 gatgtgctta ttttttgaac gatatatttt tacaaaataa gacaaatcaa tgacatgcat
    36181 aatgctgatt tttatgatga attgccataa accatagcca tgttaaggtt ttggggaatg
    36241 ataaacgcag ccagatattt accctgtcgc gttatgcggg tatcttacgc cgctgcttaa
    36301 aggagaatgc tatgtccgcc cagaaaccgg ggttgcatcc gcgcaaccgt catcacagcc
    36361 gctacgatct cgccacgctt tgtcaggtca atcctgaact caggcaattc ctcacgctta
    36421 cacccgccgg ggagcaaagc gtagactttg ccaatccgct ggcggtgaag gcgctcaata
    36481 aggcgttgct ggcccatttt tacgccgtag cgaactggga tatccccgac ggttttctct
    36541 gcccaccagt accgggccgg gcggattata ttcatcacct tgccgattta ctggctgaag
    36601 caagcggaac aattccagcg aatgccagca ttctggatat cggcgttggt gcgaactgta
    36661 tttatccgct gattggcgta catgaatatg gctggcgttt taccggtagt gaaaccagca
    36721 gccaggcgtt aagcagtgcg caggcgatta tcagtgctaa tccggggctt aaccgcgcca
    36781 ttcgtctgcg tcggcaaaaa gagagtgggg caatttttaa cggcatcatc cataaaaacg
    36841 agcaatacga cgcgaccttg tgtaacccgc cattccacga ttccgccgct gcagcgcggg
    36901 caggtagtga gcgtaaacgc cgtaacctgg ggctgaacaa agacgatgca ctgaactttg
    36961 gtggtcagca acaggagttg tggtgtgaag gtggtgaagt cgcctttatc aaaaagatga
    37021 ttgaagagag caaaggcttc gcgaagcagg tgatgtggtt tacctcgctg gtatctcgtg
    37081 gtgaaaactt accgccgttg tatcgtgctc tgaccgacgt gggcgcggtg aaggtggtaa
    37141 aaaaagagat ggcccagggg caaaagcaga gtcgctttat tgcctggacc tttatgaacg
    37201 acgagcagcg ccgccgtttt gttaatcgcc aacgttaaag cgttggctcc cccggcggcg
    37261 gttcagccgg ggccgcgccc ggagcaggca gcacctgata agtttgcact ggcgcgcgaa
    37321 cgcccgccag gtcgaaatgt tttttcacct ggctgtcgag ggcaaagcgt accgtccact
    37381 gtttgagtgg cagcgtggtg aacgaaacac gcagtgtaaa cgcggtattg cttaagccga
    37441 caatcccggc aaaattcggt tcaccaataa tcagcccgcg aatttcttcg ttttccatca
    37501 attccgctac cgcatctttc agtgcctgat tggctttatc agcatcttca tggcgatcaa
    37561 catcgtagtt tgccactacc gaaccaatac cgcggacgaa gttagcaaag gtggttatcg
    37621 aagaccacgg aatgatgtga tacgccccgg tatcctggcg cacgcccacg gagcgaatcg
    37681 acatccgttc cactgtgccg gtcaacggcc cgatagttac caaatctcca gtgttcatgc
    37741 cgttttcaaa ctgaataaat accccggtga taatatcttt caccagcgtt tgcgaaccaa
    37801 acgagatagc cagacctaat gccccggcac ctgccagcaa tggcgcgata ttgacgccga
    37861 tttccgacaa cacaatcatg atggtgatgg tactgataat caccgccagc gcgttacgga
    37921 acagcgtcag cagggtacgc gtacgggcgc tgggtagcgg gcggccatga atatccgaag
    37981 ccagccggtt ttcgatcaaa ctggcgagca ccgtccagcc aaccgccgag aaaaaaagaa
    38041 taagtgcgat acggatcagg atatctacgg ttttctgccc cgcgccgttt tgcagccagt
    38101 tccagaaatc gaacaatccc catgcgctca acagcaacat taccgccacg cagacggtca
    38161 gaatacgcgc cgttttcagc gccgccgaaa gccagccatt cagccgtttt tgcaactctg
    38221 gatagttacg ctgagtatgt ggcgagagag tgatggtttt tgccagccag cgggaaaaca
    38281 taccggaaac aaacgctgca ataccaataa tcgccaggct gcgcaccgtt gcacccatca
    38341 taaatttcag gctgttgccc ggatcgaaca acgaaaagaa aaacagcacg ataaaatagg
    38401 cgcttgccag ccagtgccac accagcgcaa aggcgcggat aaacaggctg aaaaaggcca
    38461 gcgaatgctc cgcaaagttg agcaaatgct gggtaatctc ttttttatta cgaaagatca
    38521 ggtacaacgc ccagacggtc atgcacagca taatgatgac gttcgccagc gcacctatct
    38581 gtacattcac ctgattagag ataatcggca cggccacaat caggccataa cctatcaaac
    38641 tgcttaacca gctcaggcga cgactccagt aacgggcgct ctcgtcctga atcgtgaacg
    38701 ggcgcagttc agccacgttt gggcaaaaaa tcaggcgtag tacggctttg aaaaattcaa
    38761 tcagggcaaa ggcgttgaga aacaaacttt gttggaaagc gatggtgcga ctgcccgcat
    38821 tcaggttgtc gcttaatacc tggccgacaa acaatgtcag tgccagtaac agtaggtcga
    38881 taataaacgc cccgataatc atcgccggaa gctgcagcca gttgctgcgt tcgcgatttt
    38941 tttgccgcgc ccactggccc attttgcgat acagcggcag tgcgcacaga cgaatcagcc
    39001 agtaaaaacc aaacactaat accgctaaca ttgaaaaatg ggtcaacgca ttgctgaagg
    39061 tttgtggatt aaacggctta tgcggggagc cggtgatatt gcgataaagt tgtccgaaac
    39121 gggcggaaag ggcttcacca taatggcggc tgacttcggt gaccttttgc agcacggttt
    39181 gctcttcaac cagcgtcggc ggcacgatct tcgagaccgg ttccgcaggg ggcgtcgcgg
    39241 cgacagtacg caactggtcg atcaattctt tacgcgaggt gtcgttatcc agcacatccg
    39301 ccagtgcgcc ataggccgct tttttttgtt cgatatccgg ttccggggcc ggttcagtcg
    39361 ttgagtccgt tgtcgttgtg gttgtaacgc cgggtatgga taccgcgtgg gcaggtgcgc
    39421 ccagcaggca gaagaggatg aacaggatcc accgcatgac tcctccagtg agaaaacaga
    39481 gcaaaaagat aagtatagat gctgggggag gggcgatttt aaatgagagg aatctggtgt
    39541 gcctcccttt cgggtgaaag ggaggcagag aagttaagag acgtgctgca aaaattcctg
    39601 caagcgctgg ctcggcgggt tcttgatcaa tacctgcgga tcgccatctt ccgcaatccg
    39661 gcctttgtcg ataaagatca gccgcgaagc tactttctcg gcaaaaccga tttcgtgggt
    39721 cacgatcacc atcgtcatcc cttcttcagc cagatcctgc ataaccttca gcacttcatg
    39781 gcgcagttcc gggtcaagag cggaagtcgg ttcatcaaac agcatcattt tcggcttcac
    39841 cgccagcgcg cgggcaatcg ccacacgctg ctgttgacca ccagaaagtt cggaagggta
    39901 gtgatgtgca cgttctgcca gaccgacttt cgccagcagc tcacgcgcca gtttttccgc
    39961 ctcttctttg ttcgcgccac gcacgcgtag cggaccaaac atgacgtttt ccagcgccgt
    40021 cagatgcggg aagaggtaaa actgctggaa caccataccc gcttcctggc gaatcaggcg
    40081 ctcgtcaact ttcggatcgt taaccttcag gccatcgaca atcagatcgc cggaggtgat
    40141 ttcttccagt ttgttgatgc agcgcagcag ggtcgattta ccggaaccgg acggcccgat
    40201 aatcaccacg acttcgccct gagcaatgtt caaatcgata ttgtgcagca cctgggttgg
    40261 accaaaatgc ttggagacgt ttttaaattc aatcacagga ttttcatcct tctttccaga
    40321 cgacgcagaa taaagctcag caccagggta ataatcagat agaacaccgc cacggcgctc
    40381 cagatctcaa gggcgcggaa gttaccggca ataatttctt gcccctgacg ggtcagttcc
    40441 gccacgccga taacaataaa cagcgaggtg tctttaatgc tgatgatcca ctggttaccc
    40501 agcggcggca gcatacgacg cagtgccagc ggtaaaatga cgtagcgaat ggtttcccaa
    40561 cgtgaaagac cgagcgccag tcctgcttca cgaaaacctt tgtgaataga cagcaccgca
    40621 ccacgcgtga tttccgcaat gtacgcgccg gagttgatca tgatggtgac caccgccgca
    40681 gtaaatgggt cgatgcgtaa gtcgttaaac gccatcggca gggcgaaata gataaacatc
    40741 acctggacga cgataggtgt gccgcggatc acttcaataa agaccagcgc gacgtggttg
    40801 gctatccaac caccgaaggt gcgtgcaaaa cctgccagca atccgattac cagaccgcct
    40861 gccagaccga ggaccgaaat ccacagggtc attttggcac cttcaatcag aagcggaatg
    40921 gcaggccaga tggcactcca gtcaaactgc atatgttgtt cctgttaccg tggtgtaaat
    40981 aatcaaattc agggtaatta atggccccgg agcgggtttg cgcccctcac cctaatcctc
    41041 tccccatagg ggagagggaa ctgccagtgc gttttacagg tgtagcgtta ttatttcggt
    41101 tcagtaccga accatttttt gtagatttcg ttgtaagttc cgttctcgcg cagggttttc
    41161 aacgcgccgt tgactttgtc acgcagctcg tcgctacctt tcgggaacgc aataccgtat
    41221 tgctgcgctt ccagagagtc acctaccgct ttgaactgac cgttaccggc ggttttgatg
    41281 aagtacagaa tgtttggcgt atcgtgcaga acggcgtctg cgcggttggt gcccagttcc
    41341 atataggcgt tatcgatgtt cgggaactga cgcagatctt tagttttgat gtttgctttc
    41401 gcgtaatcaa cggagccagt accgctcttc acagcaacca ctttcccgtc gagatctttc
    41461 acgcttttca catcgttatt gttagctttc accatcacta acaggccgct tttgtagtag
    41521 ccgtcagaga aatcgatcgc ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc
    41581 agatcgacgt ttttggtttg cagtgccgga atgatcccac tgaaatccat cggcttcagt
    41641 tcgtaatcca gcttcagctc tttagcgatg gcagcccaca gatcaacgtc aaagcccaca
    41701 tatttatcgc cctgtttaaa ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat
    41761 tttttatccg cggcatgaga agaaaccgca aaagccaggg tcagtgcagc cagtgaaact
    41821 tttaatacag acttcatagc atttcctttt ttctatccac gggacgatcc cctgcgtgac
    41881 attcacatat atgaaaaaat cgtgccagtt ttgcaactcc ttgatttttc gagatgcggc
    41941 aaaaacgtga tgcacgattt atagggcaat accctgaaga tgcaccattc tggggcacca
    42001 atctggtgcg ctaaaattgt gcactcaaca cagtgattat ttagcgtaaa acctgatgaa
    42061 aaaacaatct ttatgtaacg attgcgtgat gatgtggata caaaaaattt acttaatcag
    42121 ctggagatag cagatggatg cactaaataa gtgcgttgag gtgggctgca aaacaaaacg
    42181 gcctcctgtc aggaagccgc ttttatcggg tactaaagtt ctgcaccatc agcgatggat
    42241 ttattcgatg ttagactcga taaaccacag gaatttatcc aggtcgcgag acgcggcggt
    42301 caggatatct gcggtgtcgt catctttcgc ttcgccaatc gctttgcgta cgtcattagc
    42361 gacgattgcg taacggtcag ccagttcttt caggtgatcc tgaacgttgt ggatgtccag
    42421 cgggtaactt ttcagcgggg ttttgctgtt gataacttga gtggtcccca gagctacacc
    42481 gcccagctgc actgcacgtt ctgccatggt atccagatga tcgatcagtg cggtgcggaa
    42541 gccatccagc atttcatgta cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg
    42601 tttggtaatc aaagaaagat caataaactg gataacctgg cgattcagca actctactgt
    42661 tgcttttttc tcgctgtcgg agacatcgtt gcgggtataa agcagattgg tcgcttttga
    42721 tttaactaat ttagcggtac tcataatttc atatcctctt gatgttatgt cccagtaatt
    42781 aacgagacta agtatagcac cggctatgtg ttccgctatt ctggctgttc ctatcacact
    42841 aatagtggta acaagcgtga aaaacaaaac taataaagat tcaatgagtt agatatattg
    42901 ataagaacaa ttcttattta tctgctcgcc aggaattttc ggaataaata tgcttttgca
    42961 taattcgata tataaagacg gtgtagagga aaagtagcga g
//
LOCUS       DAMZII010000025        42594 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZII010000025 DAMZII010000000
VERSION     DAMZII010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.025"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(52..348)
                     /locus_tag="POA77_001329"
                     /pseudo
     CDS             complement(52..348)
                     /locus_tag="POA77_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
     gene            complement(562..1848)
                     /gene="thrC"
                     /locus_tag="POA77_001330"
     CDS             complement(562..1848)
                     /gene="thrC"
                     /locus_tag="POA77_001330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI6364794.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1849..2781)
                     /gene="thrB"
                     /locus_tag="POA77_001331"
     CDS             complement(1849..2781)
                     /gene="thrB"
                     /locus_tag="POA77_001331"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI6364795.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2783..5245)
                     /gene="thrA"
                     /locus_tag="POA77_001332"
     CDS             complement(2783..5245)
                     /gene="thrA"
                     /locus_tag="POA77_001332"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDI6364796.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5271..5390)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="POA77_001333"
     CDS             complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="POA77_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDI6364797.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5605..6291)
                     /locus_tag="POA77_001334"
     CDS             complement(5605..6291)
                     /locus_tag="POA77_001334"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI6364798.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6691..6831)
                     /locus_tag="POA77_001335"
     CDS             complement(6691..6831)
                     /locus_tag="POA77_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364799.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6927..7643
                     /gene="arcA"
                     /locus_tag="POA77_001336"
     CDS             6927..7643
                     /gene="arcA"
                     /locus_tag="POA77_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDI6364800.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7703..9055)
                     /gene="creD"
                     /locus_tag="POA77_001337"
     CDS             complement(7703..9055)
                     /gene="creD"
                     /locus_tag="POA77_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDI6364801.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9113..10537)
                     /gene="creC"
                     /locus_tag="POA77_001338"
     CDS             complement(9113..10537)
                     /gene="creC"
                     /locus_tag="POA77_001338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDI6364802.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10537..11226)
                     /gene="creB"
                     /locus_tag="POA77_001339"
     CDS             complement(10537..11226)
                     /gene="creB"
                     /locus_tag="POA77_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDI6364803.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11239..11712)
                     /gene="creA"
                     /locus_tag="POA77_001340"
     CDS             complement(11239..11712)
                     /gene="creA"
                     /locus_tag="POA77_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDI6364804.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11923..12792
                     /gene="robA"
                     /locus_tag="POA77_001341"
     CDS             11923..12792
                     /gene="robA"
                     /locus_tag="POA77_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDI6364805.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="POA77_001342"
     CDS             complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="POA77_001342"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDI6364806.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13488..14009
                     /gene="yjjX"
                     /locus_tag="POA77_001343"
     CDS             13488..14009
                     /gene="yjjX"
                     /locus_tag="POA77_001343"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDI6364807.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14094..14420)
                     /gene="trpR"
                     /locus_tag="POA77_001344"
     CDS             complement(14094..14420)
                     /gene="trpR"
                     /locus_tag="POA77_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDI6364808.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14510..16447)
                     /gene="sltY"
                     /locus_tag="POA77_001345"
     CDS             complement(14510..16447)
                     /gene="sltY"
                     /locus_tag="POA77_001345"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDI6364809.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16658..18325
                     /gene="ettA"
                     /locus_tag="POA77_001346"
     CDS             16658..18325
                     /gene="ettA"
                     /locus_tag="POA77_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDI6364810.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKYYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18632..19864)
                     /gene="nadR"
                     /locus_tag="POA77_001347"
     CDS             complement(18632..19864)
                     /gene="nadR"
                     /locus_tag="POA77_001347"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDI6364811.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19885..21267)
                     /gene="radA"
                     /locus_tag="POA77_001348"
     CDS             complement(19885..21267)
                     /gene="radA"
                     /locus_tag="POA77_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI6364812.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21316..22284)
                     /gene="serB"
                     /locus_tag="POA77_001349"
     CDS             complement(21316..22284)
                     /gene="serB"
                     /locus_tag="POA77_001349"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDI6364813.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22390..23034
                     /locus_tag="POA77_001350"
     CDS             22390..23034
                     /locus_tag="POA77_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDI6364814.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23062..24078
                     /gene="lplA"
                     /locus_tag="POA77_001351"
     CDS             23062..24078
                     /gene="lplA"
                     /locus_tag="POA77_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDI6364815.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKSPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24110..24259
                     /locus_tag="POA77_001352"
     CDS             24110..24259
                     /locus_tag="POA77_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364816.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(24534..25253)
                     /gene="deoD"
                     /locus_tag="POA77_001353"
     CDS             complement(24534..25253)
                     /gene="deoD"
                     /locus_tag="POA77_001353"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6364817.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25333..26556)
                     /gene="deoB"
                     /locus_tag="POA77_001354"
     CDS             complement(25333..26556)
                     /gene="deoB"
                     /locus_tag="POA77_001354"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6364818.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26608..27930)
                     /gene="deoA"
                     /locus_tag="POA77_001355"
     CDS             complement(26608..27930)
                     /gene="deoA"
                     /locus_tag="POA77_001355"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDI6364819.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28057..28836)
                     /gene="deoC"
                     /locus_tag="POA77_001356"
     CDS             complement(28057..28836)
                     /gene="deoC"
                     /locus_tag="POA77_001356"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6364820.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29094..30644
                     /locus_tag="POA77_001357"
     CDS             29094..30644
                     /locus_tag="POA77_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDI6364821.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30616..31479
                     /locus_tag="POA77_001358"
     CDS             30616..31479
                     /locus_tag="POA77_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDI6364822.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31592..32374)
                     /locus_tag="POA77_001359"
     CDS             complement(31592..32374)
                     /locus_tag="POA77_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6364823.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(32371..33444)
                     /locus_tag="POA77_001360"
     CDS             complement(32371..33444)
                     /locus_tag="POA77_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDI6364824.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(33566..33727)
                     /locus_tag="POA77_001361"
     CDS             complement(33566..33727)
                     /locus_tag="POA77_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDI6364825.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(33854..34459)
                     /gene="osmY"
                     /locus_tag="POA77_001362"
     CDS             complement(33854..34459)
                     /gene="osmY"
                     /locus_tag="POA77_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDI6364826.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(34852..36441)
                     /gene="prfC"
                     /locus_tag="POA77_001363"
     CDS             complement(34852..36441)
                     /gene="prfC"
                     /locus_tag="POA77_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI6364827.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(36532..37209)
                     /gene="yjjG"
                     /locus_tag="POA77_001364"
     CDS             complement(36532..37209)
                     /gene="yjjG"
                     /locus_tag="POA77_001364"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDI6364828.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(37224..37670)
                     /gene="rimI"
                     /locus_tag="POA77_001365"
     CDS             complement(37224..37670)
                     /gene="rimI"
                     /locus_tag="POA77_001365"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6364829.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(37639..38052)
                     /gene="holD"
                     /locus_tag="POA77_001366"
     CDS             complement(37639..38052)
                     /gene="holD"
                     /locus_tag="POA77_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDI6364830.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            38155..39186
                     /gene="rsmC"
                     /locus_tag="POA77_001367"
     CDS             38155..39186
                     /gene="rsmC"
                     /locus_tag="POA77_001367"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDI6364831.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            39487..39573
                     /locus_tag="POA77_001368"
     tRNA            39487..39573
                     /locus_tag="POA77_001368"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39521..39523,aa:Leu,seq:cag)
     gene            39602..39688
                     /locus_tag="POA77_001369"
     tRNA            39602..39688
                     /locus_tag="POA77_001369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39636..39638,aa:Leu,seq:cag)
     gene            39723..39809
                     /locus_tag="POA77_001370"
     tRNA            39723..39809
                     /locus_tag="POA77_001370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39757..39759,aa:Leu,seq:cag)
     gene            complement(39848..40084)
                     /locus_tag="POA77_001371"
     CDS             complement(39848..40084)
                     /locus_tag="POA77_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDI6364832.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            40225..41013
                     /gene="fhuF"
                     /locus_tag="POA77_001372"
     CDS             40225..41013
                     /gene="fhuF"
                     /locus_tag="POA77_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDI6364833.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(41051..41728)
                     /gene="bglJ"
                     /locus_tag="POA77_001373"
     CDS             complement(41051..41728)
                     /gene="bglJ"
                     /locus_tag="POA77_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDI6364834.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(41686..42411)
                     /locus_tag="POA77_001374"
     CDS             complement(41686..42411)
                     /locus_tag="POA77_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6364835.1"
                     /translation="MLPGCCKNGIFISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10143 a        11626 c        10703 g        10122 t
ORIGIN      
        1 ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg
       61 atgtttgcct ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg
      121 cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca
      181 ccagccgtgg tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgatcgcc
      241 tatctgtaat tttactgacg ggactaacgt aatttccgca gcctatgctg ccatgggagc
      301 gaccagaacc agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt
      361 ttatcattgc ccgttatggg tcgatatccg gagattacgc gatgagaacg acggttgtta
      421 ttctccgcaa tcctaatctc taagttaccg catttattga gaatttctcc tttttccctg
      481 tcgtttttct ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct
      541 gattgagata atgaatagat tttactgatg attcatcatc aatttacgca acgcagcaaa
      601 atcggcgggc agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg
      661 cagatccaac gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc
      721 ggtgccgagg aacaagccat attcgcctgg attcaactgg tcacgcagcg cacgatacgc
      781 tacggcagcg tgcggctccg aggtgtagcc cagttctttt aactcacgca ttgtctgttg
      841 cgtggtttca tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg
      901 gaacaactct tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa
      961 cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc
     1021 gttcacgttg gtcgcagcaa taaaacgttt caccggcaga ccgagtgact tcgccagcag
     1081 acccgccgtc aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc
     1141 ttcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa atctgcgcca gcaaacggct
     1201 gatgttaatc gagttagctg agtttaaccc cagcgccact ttcagttctt catcatcaaa
     1261 cgcctgcttc accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat
     1321 attgccgccc aacgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata
     1381 gaggataacc actttcacat tcggtaaacc gtagaaagca tgagccactg ccgctccggt
     1441 atcaccggag gtcgcggtca gaatggtcac tggcttatcg cccgcaatat gggtcagcat
     1501 ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc
     1561 cagacaaccg acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg
     1621 cacgcgctct tccaggattt cctgcgggat ttcatcacca ataaacgccg agaggatctt
     1681 cgcactgcgg gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc
     1741 cggcaggtca tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg
     1801 cgcaaagctg acctgctcgt tgtgatcttt cagattgtag agtttcattt agttttccag
     1861 tactcgtgcg cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag
     1921 gtagttctta cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacatagagc
     1981 gaacaaggtc gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcgaccgc
     2041 ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc
     2101 tttcatcagc ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc
     2161 cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct
     2221 ggcttctgcc gtcgagactt taatccccgg atacgccagc acccacagcc actcatcaaa
     2281 ccctggcact tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag
     2341 aaaacacggt gccacgttgt cgtaatgaat gctgccggag attcgtcctt ccagctcgcc
     2401 catcaaagcc agcaaacgag tgtcattaag cggcttgcca cagtgttcat tcatcgccat
     2461 cagcgccgcg accaccgaac aggcgctgga gcctaagccc gaaccgattg gcatattctt
     2521 ttccagagtc atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca
     2581 ctgataaacg atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt
     2641 gttgagactg aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc
     2701 aacaggtgtc accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc
     2761 cggggcataa actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat
     2821 cggcaaagac accggcagct gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg
     2881 gcagcggctg ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga
     2941 acagcggatc attaccatcc acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa
     3001 tattgccaac atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa
     3061 agagatcgtc gagctgtgac agattcgcca taaaagcggc aacatcaccc tcagcgttaa
     3121 actctgcggg cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt
     3181 cacgagcgag aatcaatagc ttacgcgcta catccatacc agaaagatca tctcgcggat
     3241 ccggttcggt ataacccatt tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt
     3301 cgtctaactt gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcatca
     3361 attcatcacc agcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa
     3421 cgttggtgtc atagaggaat ttacgccgcg atttttccgc cgcatgacgc aacagatggt
     3481 agtaatccat cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc
     3541 gcaagaagtc ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg
     3601 ggttcagcag atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt
     3661 tggcttgcgc cagttcttcc tgccagtttt ccagatttag gccatgcaca ttggtgagca
     3721 gtgccttcga gttggcaaca ccgcagacac gtaagtcgat atgtttattc ttcagccagc
     3781 tttgttgacg cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa
     3841 acacttcgat aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg
     3901 tcgcatcatc gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg
     3961 cgacaatgtt gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca
     4021 aggtgcgcat accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg
     4081 gctccagtaa gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagccc
     4141 gcacacagtc gctttgcgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa
     4201 tcagcaccac ggaaatacgg gcgcgtgaca tcgcggcaaa gacgcgcgcc gccatgccga
     4261 ccattccttt catccccggg ccggaaacgc tgaacattgc catgttattc agattggaaa
     4321 tgcccttgac cggtaattcg tcttcatcac gactggcacc aatgagcgta cctggtgctt
     4381 gaggatttcc ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg
     4441 tgcgggggtg aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca
     4501 tcgacttcaa caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa
     4561 cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt
     4621 agtcggaacc gttgcgtcca agtaccacca gttcgccttt ttcattaccg gcggtgaaac
     4681 ctgccatcaa caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact
     4741 cagcaatatc gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat
     4801 cgataacggt aacgttgtga ccacgcgctt ctaacacacc ggccataatg gcgatcgaca
     4861 ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac
     4921 taatgccatg caggacatgt tttatttggg caaattcctg atcgacgaaa gttttcaatt
     4981 gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac
     5041 gttcggcatc gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg
     5101 ccaccaggtg gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc
     5161 tggcattgct ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg
     5221 taccgccgaa cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag
     5281 cccgcactgt caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt
     5341 acctgtggta atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt
     5401 gtactctgta atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga
     5461 cctaagtcaa taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca
     5521 gacactcttt ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct
     5581 gaaaaatact tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa
     5641 acggtgcaac attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc
     5701 gaccagtttt atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg
     5761 taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa
     5821 tgttgctaat tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc
     5881 cgccaccatc ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc
     5941 ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa
     6001 cagcggcacc agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt
     6061 ggtggcgaca gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat
     6121 attatcaata atatcaccag atccatgtgc gacccagcgg gtggctggct ccagatgtgc
     6181 ctgactatcg acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc
     6241 cccaatattt tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact
     6301 cttcttgacc aaataaccgc gaacagggtg atcactgtca acttattacg cggtgcgaat
     6361 ttacaaattc ttaacgtaag tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa
     6421 ctatttcctg actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta
     6481 cttaggataa ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg
     6541 tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat
     6601 gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt
     6661 tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac
     6721 agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt
     6781 gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg
     6841 atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga
     6901 tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg
     6961 agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg
     7021 aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga
     7081 tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc
     7141 aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg
     7201 gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga
     7261 ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc
     7321 gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga
     7381 tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact
     7441 tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc
     7501 gtgagctgaa accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt
     7561 tcgaatctac gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct
     7621 tctgtggtga tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta
     7681 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt
     7741 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgggtcacaa
     7801 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
     7861 cagagttgag cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca
     7921 tgctgttgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
     7981 tcagactggc gattatccat gccacggtaa aaccggtatg ttcagaaagc gccagcaaga
     8041 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaagcgtt
     8101 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagcgcaatc agcagaatgg
     8161 catacttagt cgcccggtca gttaattggt attgatcggc tggcgtcgtc actgcgacgc
     8221 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
     8281 tatttgcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt
     8341 tggctggcaa aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
     8401 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagggcca
     8461 tattcagctt caggttctgc ttgcgccagt ccccttcggg taaagggata tgcacgccct
     8521 gcccgccttg ctctaacccg gtaccgggtt cgatggtcag cgctgttccg ttaacttcag
     8581 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aatggcttgc
     8641 ctaaggtgat atttggtgcg tcgagttcgc taagacgcga aacatcgaaa tcggctttta
     8701 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttcaacgt
     8761 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
     8821 gctccacggt tttatcgtct tcctgcaccg tataaagctc ggtcacagga atagcgatga
     8881 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
     8941 tacggtaatc ggcacgttcg acaatcacct gccgaatcag cattatcgga atcaacaaca
     9001 gcaatactgc accaaacagg gtggtcattt tccagaacag gggggatttc aacatagtct
     9061 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
     9121 agtgacggtg aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
     9181 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
     9241 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
     9301 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
     9361 cgcttagcgt tatacgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc
     9421 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcgtaacat
     9481 gtagagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcggaata
     9541 atgccgccac atcaacagca gtcaaaacga cttcctgacg attctccagt cttgcctggc
     9601 gtagtaacgt ttccaccagt gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa
     9661 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
     9721 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
     9781 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
     9841 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
     9901 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc
     9961 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    10021 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg
    10081 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    10141 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa
    10201 cggctttatt tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat
    10261 attcattgcg cactttgtta atgccaccga tattggcgcg aaacgggcga tgttgtagct
    10321 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    10381 ccagctccgc cagcaacgtt gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc
    10441 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    10501 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc
    10561 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    10621 caacgtttta atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    10681 gctatccatc agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    10741 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    10801 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt
    10861 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    10921 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    10981 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    11041 gccgctgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc
    11101 tttatccagc accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    11161 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    11221 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    11281 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    11341 agcatcataa aagcgcacga cctgtaacga cttaaagacc agggacgtac gttttttgaa
    11401 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    11461 ttgctgacaa gaaatggccg catcggaggt atcttccgcc aggcccaatc cccctttaat
    11521 acccccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    11581 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    11641 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    11701 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    11761 aataacatat tgattaagaa caaaatctca atacttttat ttccgtcaag ccctaaaaca
    11821 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    11881 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    11941 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    12001 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    12061 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    12121 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    12181 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    12241 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    12301 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    12361 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    12421 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    12481 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    12541 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    12601 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    12661 tgcatgccaa tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    12721 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    12781 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    12841 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    12901 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    12961 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    13021 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    13081 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    13141 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    13201 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    13261 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    13321 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    13381 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    13441 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    13501 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    13561 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc
    13621 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    13681 tacttccaga ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt
    13741 tcagttgggt ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc
    13801 cgttacctgc ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt
    13861 cgcgttatac cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg
    13921 ccgggaaact cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt
    13981 ttcacaatgc cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca
    14041 ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg
    14101 cttttcagca acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg
    14161 ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctgg
    14221 ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc
    14281 tcatctggcg tcagcatcag atttaacaac ggtaaatgga gatcgttttg gtaggcattc
    14341 ttaagcaggt cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa
    14401 tagggtgatt gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc
    14461 cccggttgta ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac
    14521 gtccccattc cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag
    14581 cgtcataagc cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga
    14641 caaatgccac tgcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgccctg
    14701 gtccggcgtt ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat
    14761 attgcagata actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc
    14821 tactataacc gggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct
    14881 gcatcaggcc gctggcccct accggtgatt tcacttttgg attccaggcg ctctcctgac
    14941 gggcaatcgc catcgcatag ctttgcggga tctccttacc gctggtgtag cgtttgaaaa
    15001 gatcgttgta agccagcggg aatcgctctt ccagatgatc ccatagcttc ccggcgatcg
    15061 ttgcctgaac gctaagatcc caccattggt tgttgaaagc ataccgcgcc agttgagcct
    15121 gctctgtttt tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga
    15181 gattccagta catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt
    15241 caacattctg cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg
    15301 caaccatcgg gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag
    15361 cttcacgtcc gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt
    15421 ttgcttccat cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa
    15481 gtgccattcg cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat
    15541 cgcgccattt cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat
    15601 cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca
    15661 tcagccgtgc attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt
    15721 gacgggtaaa atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt
    15781 tcgccagtga aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca
    15841 gcactgttac caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg
    15901 ccagcggatc ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag
    15961 gctggctctt gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc
    16021 cggtgttcca tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg
    16081 ggctaaaggc taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag
    16141 attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca
    16201 ccgccggttg attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg
    16261 gataatcctt cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg
    16321 cctgcttgat ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc
    16381 tgctgaccgt cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt
    16441 tttccacaag cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag
    16501 atttatgcta accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact
    16561 gcgaaggtga tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa
    16621 actcatcatc acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat
    16681 gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag
    16741 tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct
    16801 gctgcgcatt atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga
    16861 catcaagatt ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga
    16921 gtccattgaa gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta
    16981 tgcgctgtac gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct
    17041 ggaagagatc attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc
    17101 ggatgcgctg cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg
    17161 tcgtcgcgtg gcgttgtgcc gcctgcttct ggaaaaacca gacatgctgc tgctcgacga
    17221 accgaccaac cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt
    17281 cgaaggcacc gttgtggcga ttactcacga ccgttacttc ctcgataacg ttgcaggctg
    17341 gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct
    17401 ggagcagaaa gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc
    17461 gatcgagaaa gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa
    17521 agcacgtctg gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac
    17581 caacgaactg tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa
    17641 cctgcgtaaa tactacggcg atcgcctgct gattgatgac ctgagcttct cgatcccgaa
    17701 aggggcgatc gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat
    17761 gatctccggt caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact
    17821 ggcgtcggtt gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt
    17881 ttccggcggg ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt
    17941 tggccgcttt aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg
    18001 tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct
    18061 cgacgaacca accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct
    18121 ggagttcccg ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc
    18181 cacgcacatc ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac
    18241 tgagtacgaa gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat
    18301 caagtacaag cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat
    18361 ccggcctaca aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg
    18421 taagcgtcgc atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg
    18481 cctacaacag ccttagtatc cgcaaattcg tagcgggtca cccgtgctga ttcttttaaa
    18541 ccttgtttaa tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt
    18601 tatacctcgc ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca
    18661 ccaactccac gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt
    18721 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    18781 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    18841 ccagatcgaa acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt
    18901 actttttgca gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg
    18961 catatttcac tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg
    19021 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt
    19081 cccacgcact ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc
    19141 tcgactcgcc accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa
    19201 tatattccca gtagcggaac ggattttcgc ggatctgcgc accgctgata ctcataaagg
    19261 tacgtttcgg atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat
    19321 cggcttcttc cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt
    19381 tgatgccgtt gctccacaca tcccagccgt gcggatacgg ctccatgccc tcttcgttga
    19441 aagcatgaat gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat
    19501 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    19561 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    19621 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    19681 gtcggggaaa ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc
    19741 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    19801 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    19861 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    19921 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    19981 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg
    20041 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    20101 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc
    20161 ggttaccttc acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc
    20221 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    20281 cacgcggcgc ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    20341 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    20401 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    20461 gacgcccagc tcattcaccg cgccgaagcg atttttatgg ctgcgcaagg tgcgaaaacg
    20521 ggagtcggca tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg
    20581 gccagccagc gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt
    20641 tttggcgaag cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    20701 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg
    20761 ctcttcttcg gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt
    20821 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    20881 agtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt
    20941 ggatttcccc gcacctgggt taccgccaat cagaatggcg cttcctggca ccacgccgcc
    21001 gcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    21061 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    21121 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    21181 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    21241 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    21301 atggcgggcg agcaattact tctgattcag gctgcctgag aggatgcaga atacccccat
    21361 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    21421 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    21481 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt
    21541 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    21601 cagttcattg gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa
    21661 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    21721 ttgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    21781 agcgcctttc agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg
    21841 catcgcccgt tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc
    21901 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    21961 gcgcaggtgc gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg
    22021 cgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    22081 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    22141 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    22201 cacttcatca ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag
    22261 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    22321 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    22381 gggtggaaga tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg
    22441 ttctgtcttg ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac
    22501 cagcgacaac gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg
    22561 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    22621 ctcgatcagt taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    22681 gatcttatcg cccgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt
    22741 aaaaaagcgg gcggctattt taaccagcag attgtagaac caattgccgg gaaaaacgga
    22801 ccgctcggct atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag
    22861 gtggataaca ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta
    22921 gtgctgaccc gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg
    22981 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    23041 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    23101 ccgtggttta acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc
    23161 gttctgtttc tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg
    23221 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc
    23281 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    23341 ccggagtacg ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc
    23401 gtcagcgccg aagcgtccgg gcgtaacgat ctggtggtga aaaccgccga aggtgaccgc
    23461 aaagtctcag gatcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg
    23521 ctgctcaatg ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg
    23581 gcggcgaaag gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    23641 gggatcaccc atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc
    23701 gagcgtgtag aagcggaaat catctccccg gacaaatcgc cagacttgcc aaacttcgcc
    23761 gaaacctttg cccgtcagag tagctgggaa tggaacttcg gtcaggctcc tgcattctcg
    23821 catctgctgg atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa
    23881 ggccatatca cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc
    23941 ctcgccgggc gactgcaagg cggcctgtac cgcgcggata tgctgcaaca agagtgcgaa
    24001 gcgctgttgg ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata
    24061 gcgggggcgg taaggtaact cgctgattaa aataccaaag gaagggaata tgatcgatct
    24121 ggaaaaccag gagcgggaga ttatcaatat aatgttaagc cagcgtattt cctggctagc
    24181 cgccgtgcgt atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc
    24241 gattaattct ctaaaataga tcgaaaagac ggaacggttt agctcaaaca tcaaaagcaa
    24301 aatggcagaa atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc
    24361 tgaacataaa tgaatagatt cgtgaataga tacttgaata gattttattt tctatccata
    24421 tgaaaaataa tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg
    24481 gagcagtctc ccactccggc ttcacacgac aatcgccttt cagcgaaaca caattactct
    24541 ttatcgccca gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga
    24601 cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt cagacacggt acagatggtc
    24661 agggctttcg cgccaaattc tgcagcgacg ccgtagatac cagccgcttc catttccacg
    24721 ccgaggatgc cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg
    24781 tcagcggaga acaggttacc aacgcgagca tcaatgccca gtgctttagc cgcgtctacc
    24841 gcgttacgca ccatgtcaaa gtcagcgata gcggcaaagt catgatcttt aaaacggatg
    24901 cggttaactt tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg cagttttacg
    24961 tgcggcagaa ctgcgccaca ggaacccacg cggataattt tcttcacgcc gaaatcggtg
    25021 atcagttctt tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa
    25081 attttgcggc ctttataagt accggtgaag cccagcatac cgcgaacgtt gttcacttca
    25141 cgggcatctt caaggaaagt ttcagcaata tactttgcac gcagcgggtc gcctggcatc
    25201 aaaactacgt cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta
    25261 tccttatcta catgttttca ccctctccct gagggagagg gccggggtga gggcatcaag
    25321 ccgcacccat tatcagaaca tggctttgcc atattccata tcagaagtac caaaatattt
    25381 tgccagagtc tggccgatat ccgcgaaggt ttcacggtga cccagtgagc ccggttttac
    25441 tttcgggccg taaaccagta ccggaatgtg ttcacgcgtg tgatcagtac cggtccaggt
    25501 cggatcacaa ccgtggtcag cagtgaggat caggatgtcg tcatcgcgca gcagagacat
    25561 cagctccggc agacggcggt cgaacagttc cagacccgcc gcataacctg cgacgtcgcg
    25621 acggtggccc caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc
    25681 cgcttctttc atctctttga tggtggcgtc aaacagcgcg tccaggccag ttgctttcac
    25741 tttcttggtg ataccgcagt tggcgtagat gtctgcaatt ttaccgacag aaaccacctg
    25801 accgtgtttt tcatcaacca gtttctgcag cacggtcggt gctggcggct caacagccag
    25861 gtcgtgacgg ttaccggtac gctggaagtt accggctttg tcgccaataa acggacgagc
    25921 gataacacga ccgatgttgt agccgccgtt ggtcagctct tcacgggcga tttcgcacag
    25981 ttcgtagagt ttatccaggc caaaggtttc ttcgtggcag gcaatctgga acacggagtc
    26041 agcggaggta tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gctgatccag
    26101 aatgaccgta ccggaagagt ggcagttacc gagataacca ggcaggttag cgcgttcaac
    26161 cagtttatcc agcagctctt gcgggaagct gttttcgtga tcggagaagt atccccactc
    26221 aaacagaacc gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga
    26281 catttcgtgc gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg
    26341 aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc agacgggtca gatttggcag
    26401 attgagcggg cctttacgac cgttatcagc ttcgccttta gcacaagctt ctgcgatatg
    26461 acccagggtg tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc
    26521 gaatgagtcc agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca
    26581 aatttaaatg atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt
    26641 gctttccggt gctttatcgg caagtttaat tgccgctttc accgctttcg ccgcttcctg
    26701 ccagctgttt tcgtctttcg cgtggataac cgccagcgga cgctgaccgt ctacctggtc
    26761 gcccagacgc gccatatcag taaagccgac gctgtaatca atggtgtcag atgcctgacg
    26821 acgaccgccg cccattgcaa ccactgccat ccccagcgcg cgggtatcca tttcactgac
    26881 aaaaccttcg gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc
    26941 gtagttctca acgaagtcgg ttgggccttt ttgtgccgct accatacgac caaagacttc
    27001 tgccgcttta ccgttgtcca gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt
    27061 cgccagtttg ccggagataa gcatctccac gcacagcgcc atcgtgacat caaacagacg
    27121 cgggttacga tattcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc
    27181 tgcactggag gccagtacct gattcatatc ggtgagcagc gcggtggtgc gcacgccagc
    27241 gccgttagcc acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat
    27301 aaacgcgccg ctacccactt tcacgtccat caccagcgca tccagacctt ccgccagttt
    27361 cttcgccagg atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcacg
    27421 ggtcgcgtag aaacgtttat ccgccggagc cagtgagctg gtctggccga taatcgctac
    27481 gccgacgtct ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg
    27541 gatggattcc agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cggagatcat
    27601 cggaatatag ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tcacatcgcc
    27661 gacgccgccg gtggagtgtt tatcaacaat cgggccattc agatgcaggc ttttccagtc
    27721 gagaacggtt cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat
    27781 atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc
    27841 gcgaataccg ttgataaaga aacgaatttc ttcatcgctt agcgcatgac catcacgttt
    27901 tttacgaata atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc
    27961 ggcttttgcc ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc
    28021 tgataagcgc agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc
    28081 gtcaccgtgg cccagcgctt tcaacaggct tgccagcagg ctggaagcac caaagcggta
    28141 gtgacgcgca tctgcccagt cagcaccgaa cagttcatct gcgatggcga gatatttctg
    28201 cgcatcttcc gcagtacgca cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc
    28261 catatcacgg atcacttcca tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac
    28321 tttaccggta gaggttttga tgaagtccgc acccgctttg atggagattt cagacgcttt
    28381 acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt
    28441 cgctgccgcg caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgttacc
    28501 cgccatcagc gcgcggtacg ggaacacaac gtcaacttca tcagcaccgt aggcgattgc
    28561 cgcacgggtt tctgccagcg cgatttcgat gtcatcgtta ccgtgtggga agttggttac
    28621 cgtagcgatt cggatttccg gggtgccctg ctctttcagc gttttgcgag caatcgggat
    28681 aaagcgagga tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca
    28741 cagagcaatt actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt
    28801 cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa
    28861 taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata
    28921 tacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca
    28981 aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac
    29041 atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca
    29101 cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta
    29161 gcccggagca gaagcgccat tttctggctc tggaagcaga aaacaatctg ccttatccgc
    29221 agcttcctgc cgaagcccgc cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag
    29281 gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgcccgtttt ctggcgaacg
    29341 gttccgaatg gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga
    29401 ccattcttta ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg
    29461 atgcgttgtt gcaaccgtat gttagaattc taacacaaga cgagatcgat gttcgaataa
    29521 aacgtttctg gcgttacctc gacagaaccc tgccagacgc ctttatgcac gccaatatcg
    29581 gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc agacgcagag ttgaagcagg
    29641 tttcaccgaa cctgaccttt atctacgatc ctgaaatcac ccctgatgac ctgctgctgg
    29701 aagtggcgaa gaacatctgt gaatgtagca aaccgcacat cgccaacggt ccggtgcatg
    29761 ataaaatttt cacaaaaggg ggctacggga ttgtgagctg ttacaactca ctgccgctgg
    29821 cgggtggtgg cagcacgctg gtacgcctta acctgaaagc cattgccgag cgcagcgaat
    29881 cgctggatga cttctttacg cgcactctac cgcactactg ccagcagcag atcgccatca
    29941 tcgatgcgcg gtgtgaattc ctctatcaac aatcacactt ctttgagaat agcttcctgg
    30001 tgaaagaagg gctgattaac cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg
    30061 cggaagcggt taacttgctg tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag
    30121 ccgccgcaaa tgaagtgggt tatcgcatca gcgcgcaact ggcggagttt gtcgccaata
    30181 cccccgtgaa atatggctgg caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt
    30241 ccgatatcgg caccacgccg ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca
    30301 cccatctgca aactgtcgcc ccgcatcatg cttattatta ttccggcatc agcgacattc
    30361 tgacgctcga cgaaaccatc aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg
    30421 cctttaaagc cggaatgcgt gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg
    30481 ttaccggtta tatggtgcgt ttgtcggact tagaaaaata tcgcgccgaa ggttcacgca
    30541 ccaacaccac ctggctgggc gaagaagccg cacgcaacac tcgtattctg gaacgccagc
    30601 cgcgcgtgat aagccatgaa cagcagatgc gctttagtca gtaagattat ccccttctcc
    30661 tgcgttgacg ggccaggcag tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc
    30721 aaaaactgtc acaatccgtg gacgatggga cgttgcaatg actgtgggga gtgcgtgcca
    30781 cagtgtccgc atcaggcgtt gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt
    30841 tgcgagcagt gtgatacctg cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa
    30901 tccatgagcg tggacgaagt gcttagccat gtccgcaaag cggtgctgtt tattgaaggg
    30961 ataacggtaa gcggcggcga agccacgacc caactgccgt ttgtggtggc gctgtttaca
    31021 gccatcaaaa acgatccgca actgcgccac ctcacctgcc tcgtggacag taacggtatg
    31081 ttgagcgaaa ccggctggga aaaattactc ccggtgtgcg acggcgcgat gctcgatctc
    31141 aaagcgtggg ggagcgaatg tcatcagcaa ttaaccggac gcgataatca gcagattaag
    31201 cgcagcatct gtttgctggc agagcgcggt aagctggcgg aactgcgttt gctggtaatt
    31261 ccagaccagg tggattattt gcatcacatc gatgagctgg cgacgtttat caagagactt
    31321 ggcgatgttc cggttcgcct gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa
    31381 agctgggcga gcgccacgcc ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc
    31441 ggggtgagcc ggttgatatt tccggcgctc tatttgtgac atgccggata cggcctacgg
    31501 ttcggcacaa acctgtaggc atgataagac gcgacaagcg tcgcatcagg cattatgcgc
    31561 cgactgccgg atgctgccta cgaagggcaa actacccact aacgctaaac accgcatacg
    31621 tattattaag caacacttcg gcaatctcat ccgccggttc cgagcgcaac tcgcacagca
    31681 cggcaaatac ccgggcggcc tgttccgggc ggttagcctg cccctgaaaa ccattgagcg
    31741 gcatatccgg cgcgtcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat
    31801 cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca
    31861 gctgtacaaa ccgttcggcc tgttgcaggc tgccggaaaa accgtgaacc acgccagtgc
    31921 gcgataaatc gtggcgttta agatgcatcg ccagtttgtc gtgtgtgcgc cgggaatgaa
    31981 ggatcaccgg cagatcgtag cgtttcgcca gtttcagctg ttcgtcgagt aaccactgct
    32041 gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca
    32101 ccttcgccgg acgcctttcc agcgcctgct gtagctgatc aagagacaca tcgctatgtt
    32161 tttccaacat ccccggatgc aaccccaatg cggcatacag cggttgatat ttttccgcta
    32221 acgcctgcac gcgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc
    32281 ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc actaaacggc gggaaatcaa
    32341 aatggcagtg ggtatcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg
    32401 gtcgttcgcc tgcggagcat cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc
    32461 aggcggaata acgatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc
    32521 tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag
    32581 tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt
    32641 cggtggataa atttcgaata tccgcagctt gcccggtggt ttctcaataa attgctgaat
    32701 gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc
    32761 acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac
    32821 agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc
    32881 atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaaa
    32941 gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa
    33001 gtaattaggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc
    33061 aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag
    33121 ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg
    33181 ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc
    33241 cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc
    33301 gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca
    33361 caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag
    33421 cgttacaggt attcgctgcc ccacctctgc cttcctgttt ttgttgtgaa agaatgacaa
    33481 agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga
    33541 taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac aaactcacca
    33601 ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca
    33661 gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac
    33721 gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc
    33781 actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac
    33841 gaattacgac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    33901 ctttggcgat actttcagca cggtcgctct gtgcctggga atcgacggta ccggagagct
    33961 gaaccacgcc gtcggtggtt tcaactttca catgacggga agggacgata tcgtccgcca
    34021 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    34081 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacccct tcaacgcctt
    34141 tcgccacttt cactgcctct tcggcctgag cctggctttc aacgaaaccg ctcagggtca
    34201 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    34261 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    34321 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgtgcgttg ttttccgcgt
    34381 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    34441 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctggca
    34501 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    34561 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    34621 atcggtagaa tgacaatcga cggctttaga tactgatatc tacgcattga acggtattta
    34681 acgccgtcag aaatgtcatt actttgttaa atatagttca caattttgaa accgctcggg
    34741 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    34801 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    34861 gcgggtctgg tggaactgaa cgtcgggata acgttcctgc gccaggcgca ggttgaccat
    34921 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    34981 acgcttgaac tcttcgaatt tcttcgcgtc cgcacattct acccagcggg cagtggcaac
    35041 gttgactgat tcatatactg cttcaacgtt gtattcgctc ttcaggcgcg ataccaccac
    35101 atcaaactgc agcacaccaa cagcaccaac gatcaaatcg ttgttggaga tcggacggaa
    35161 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    35221 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    35281 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatagtgc cgtggttgtg
    35341 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcgc ccgccataaa
    35401 ggtcagcgcg tcggagataa ccacatcttt cgcagtacgc acctggcgca gcttcatgcc
    35461 tttttcatat ttaccggata ctacgcgcat aaacgccacg cggtcgcggt gtttcgggtc
    35521 catgttggcc tgaattttaa atacgaagcc ggtaaatttg tcttcgctcg cctctacggt
    35581 acgggtatca gtctgacgcg gcatcggcgc aggagcccac tccaccaggc catccagcat
    35641 atgatcaaca ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    35701 aaggaacagt tctttgtcga actcgttaga cgcgcctttc accagttcca gttcgtcacg
    35761 tagctgctgt gccagatctt caccaaccgc agcgtcgaga tccgggttat tcagcccttt
    35821 aacaatacgg acttcctgaa tcgtgtggcc tttaccgctc tgatagagat aggtttcgtc
    35881 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaatcggcc aggtgatcgg
    35941 tgcgcagccg attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    36001 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    36061 catcagctta cgggtacgat cttcaacacc ttttgcagcg tcgataacca tcaggcagca
    36121 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    36181 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    36241 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    36301 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    36361 agtcttaccg gcatccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttg
    36421 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    36481 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    36541 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    36601 tggttgctcg cgatggtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    36661 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    36721 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    36781 ggaaatcacc agcaaatcga agtaatcacg caggcccgtg cgttccagac gcacttgctg
    36841 caaggcgcta aagccgttgg tgatgatgcc gattttggcg ttgccacgaa tggcgttaag
    36901 cagagaaacc gcgcccggca gcggcgtgca gatttccgcc atcgcattaa taaaggcatc
    36961 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcaaaac gcccgtgctg
    37021 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    37081 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    37141 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    37201 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    37261 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    37321 ctttcgtaca gggcaatggc ggcagcgttt gaagcacgga cttccagcca tagtgtcgcc
    37381 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgcccttcc caatccctga
    37441 cgctgatagt caggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    37501 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    37561 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    37621 tggtaagccg ccggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    37681 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aaatcggtga gcgccggtga
    37741 tgccacctga gcgccttcca gtgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    37801 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cctggtcggg
    37861 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    37921 gtttgccacc atcaccagac ggacgtgtgc cgggatcgca atggcaatct ccccctgcaa
    37981 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    38041 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    38101 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    38161 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    38221 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    38281 cgtgctcata cccagcaatt ccaccactgg caggtattaa gccgccagat gggggataac
    38341 gcccgtttca gtctggtcgc cacggcggat gacgtcgcag attgcgatac gctgatttac
    38401 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    38461 ccggtgggga cggatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgccgag
    38521 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    38581 tattttggtc gtctggaaaa acagccggta tttgatgccg ataaattctg gggcgaatac
    38641 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    38701 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    38761 gtcggctgtg gcgcgggggt gctttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    38821 ctcaccttgt gcgatgtctc tgcgccagcg gtagaagcca gccgcgcaac acttgcggcc
    38881 aactgtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggtcgtttt
    38941 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    39001 caaacgctga ttcgcggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    39061 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    39121 atcgcgcaaa ccgggcgctt caaggtgtat cgcgccatta tgacccgcca ggcgaagaaa
    39181 ggttaagtat cccgccgaat actgtgtcgg atgcggcgta aacgccttat ccgacctaca
    39241 aaacctgcac gttagccctt cgtaggccag ataagacgcg cccgcgtcgc atctggcatc
    39301 tgcacccaaa taccgtgtcg gatgcggcgt gaacgcctta tccgacctac aaaacctgca
    39361 cgctagtttc ctgatggaca tttttccagc aattacacct ccggcgataa ttaactattg
    39421 acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga
    39481 tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg
    39541 gacgtggggg ttcaagtccc ccccctcgca ccaaaaacca cgttgatatt gctcgcactg
    39601 ggcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgttc ttacggacgt
    39661 gggggttcaa gtcccccccc tcgcaccaaa cgaggcgata tcaaaaaaag taagatgact
    39721 gtgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc cttacggacg
    39781 tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcctttctt gtttcttcct
    39841 tgatttttca tccctgattc aaattcatta tcatcagcat gatggcagca ataagcgcca
    39901 ggcacgatgg tagcaaaacg taatgccgta actgtttgtg atacagcatc accgacgtca
    39961 atactaatcc catcagaatg acaatccgcc actgatccgg tgataaatcc gcatacatca
    40021 gccctgcaat cagcaatcct ggcagcaaca ctccccagcc actgcccagc gtacgttgca
    40081 acatggtttc atctctttca ttgataatga taaccaatat catatgataa tttttatcat
    40141 ttgcaagcca gagaaatccc ttgctatcgg gtaaacctat cgctaggatt agcaatcatt
    40201 atcatttaga ttactatccc gattatggcc tatcgttccg caccgctcta tgaagatgtt
    40261 atctggcgaa cgcatctcca gccacaggat gctgggcttg cacaagccgt acgtgcgacg
    40321 atcgccgaac atcgtgaaca tttgctggag tttatccgcc tggatgaacc cgctccgctt
    40381 aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct actcgcggtc
    40441 tattccgatc atatctatcg caaccaacca acgatgatcc gcgagaacaa accgctgatc
    40501 tcactatggg cacaatggta tatcggcctg atggtgccac cattaatgct ggcgctactg
    40561 acgcaggaaa aggcattaga tgtgtcgccg gaacatttcc atgctgagtt tcacgaaacc
    40621 ggccgtgccg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac accacattcg
    40681 ccgcagcagc gaatggaaag gttaatcagc caggcgctgg ttccggttgt gcaggcacta
    40741 gaagcgaccg gagagatcaa cggcaaactt atctggagta ataccggtta tttgatcaac
    40801 tggtatctta ctgagatgaa gcaactgctc ggcgaggcga cggttgaatc gctgcgccat
    40861 gccctctttt ttgagaaaac gctcacgaac ggtgaagata atccactctg gcgtaccgtg
    40921 gtactgcgcg atggcctgct ggtgcgccgc acttgctgcc agcgttatcg cttaccggat
    40981 gtgcagcaat gtggcgattg tacgctgaaa tgaaagcact gccggggaag taaacccggc
    41041 ataatgcgga ttaataggga tgcaacacat tacttgcttc gcaatgccgc atacataaca
    41101 gaatatctgc ggcctccaac aaccctgcat cagaactgac gcccagtttc gacatcacat
    41161 taaatttatg tgcacggatc gttttgatat tgcgtttaag ctgctcggca atttgtgtca
    41221 ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt aacgtccggc
    41281 tttggttaat gtaccactga ttgttcagtc ggtcggtcgc ctgacgtaca ccatttaatg
    41341 acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat acaccgtcca
    41401 gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat atcatcatcc gcaataacta
    41461 aacgccgggt acgcggaaac ttaatcgcca gttcagtcag gcaagataat ccctctctgc
    41521 gctcacttct catggcagaa aaagaaaaaa tgaccgccgc aaatgagatc cgcgacatgg
    41581 catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggtta cccgcaaact
    41641 ttctgaataa actctcaata ccgatactgc tcatgacgca tttttctatg agtgcgacat
    41701 ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag aaggcaagtt
    41761 aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt tgctgtgaat
    41821 tgccagtcga cgcatcgcac tgtttttttg ggcgctgata gttttattac ttttcttaag
    41881 caatgaagct atctggttga tgccccatcc tttacctaat agccgcagta ccttgcgctc
    41941 tgaaagagtg agaatgacgg agcgatagct aaaatcatga gtttcattca ttgccggaga
    42001 ggtcagcatg gcgctgatac gctctgcgtg cgtattgccg gaacgtacgg tcttgaccag
    42061 attctcaatg ggttcaacat cagacaataa cgtcgccgta gggcacatga gcaattctac
    42121 tgcctgggaa taccaggagc gtgacaccat gaaaacccag tgaatttccc gatattgtga
    42181 gattaaagaa taataatgtt cacaaacaga acgtggatct tcactttcac cggctaaatc
    42241 agcaatgact aatccggaac gacgtaattg taataaggta aggtcctccg cagaggcgct
    42301 ggatattatt tcatattcag ggaagtgagt cctcatgacc tcttttagcc ctgcttgcat
    42361 aacaggtatt ttactgataa aaattccatt tttgcagcat cctggcaaca tatttcctcc
    42421 acatccactg tctaaaaatt atgatggttt attcaggata ttcattcctt aaatgtttcc
    42481 agcaaattaa tagattaagc gaataaatta tccttagcat tcactttcat atcagcatac
    42541 tcctgctgca caacaactcc actgcatgtg tcagaaggtt agcatcaaat gata
//
LOCUS       DAMZII010000026        42087 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZII010000026 DAMZII010000000
VERSION     DAMZII010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.026"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            371..1588
                     /gene="alaA"
                     /locus_tag="POA77_001375"
     CDS             371..1588
                     /gene="alaA"
                     /locus_tag="POA77_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDI6364836.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1672..2271
                     /gene="yfbR"
                     /locus_tag="POA77_001376"
     CDS             1672..2271
                     /gene="yfbR"
                     /locus_tag="POA77_001376"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDI6364837.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2330..4162)
                     /locus_tag="POA77_001377"
     CDS             complement(2330..4162)
                     /locus_tag="POA77_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI6364838.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4249..4899)
                     /gene="hxpA"
                     /locus_tag="POA77_001378"
     CDS             complement(4249..4899)
                     /gene="hxpA"
                     /locus_tag="POA77_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDI6364839.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4910..5404)
                     /locus_tag="POA77_001379"
     CDS             complement(4910..5404)
                     /locus_tag="POA77_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDI6364840.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5487..5942)
                     /locus_tag="POA77_001380"
     CDS             complement(5487..5942)
                     /locus_tag="POA77_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDI6364841.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6280..7482
                     /gene="ackA"
                     /locus_tag="POA77_001381"
     CDS             6280..7482
                     /gene="ackA"
                     /locus_tag="POA77_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI6364842.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7557..9701
                     /gene="pta"
                     /locus_tag="POA77_001382"
     CDS             7557..9701
                     /gene="pta"
                     /locus_tag="POA77_001382"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6364843.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9891..11411
                     /gene="yfcC"
                     /locus_tag="POA77_001383"
     CDS             9891..11411
                     /gene="yfcC"
                     /locus_tag="POA77_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDI6364844.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11444..11986)
                     /gene="yfcD"
                     /locus_tag="POA77_001384"
     CDS             complement(11444..11986)
                     /gene="yfcD"
                     /locus_tag="POA77_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDI6364845.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12044..12595)
                     /gene="yfcE"
                     /locus_tag="POA77_001385"
     CDS             complement(12044..12595)
                     /gene="yfcE"
                     /locus_tag="POA77_001385"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDI6364846.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12651..13295)
                     /gene="yfcF"
                     /locus_tag="POA77_001386"
     CDS             complement(12651..13295)
                     /gene="yfcF"
                     /locus_tag="POA77_001386"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDI6364847.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIEEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13431..14078
                     /gene="yfcG"
                     /locus_tag="POA77_001387"
     CDS             13431..14078
                     /gene="yfcG"
                     /locus_tag="POA77_001387"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDI6364848.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14135..14497
                     /gene="folX"
                     /locus_tag="POA77_001388"
     CDS             14135..14497
                     /gene="folX"
                     /locus_tag="POA77_001388"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDI6364849.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14518..15411
                     /locus_tag="POA77_001389"
     CDS             14518..15411
                     /locus_tag="POA77_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI6364850.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15459..16361)
                     /gene="rpnB"
                     /locus_tag="POA77_001390"
     CDS             complement(15459..16361)
                     /gene="rpnB"
                     /locus_tag="POA77_001390"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDI6364851.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16558..17331)
                     /gene="hisP"
                     /locus_tag="POA77_001391"
     CDS             complement(16558..17331)
                     /gene="hisP"
                     /locus_tag="POA77_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDI6364852.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17339..18055)
                     /locus_tag="POA77_001392"
     CDS             complement(17339..18055)
                     /locus_tag="POA77_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6364853.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18052..18738)
                     /gene="hisQ"
                     /locus_tag="POA77_001393"
     CDS             complement(18052..18738)
                     /gene="hisQ"
                     /locus_tag="POA77_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDI6364854.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18828..19610)
                     /gene="hisJ"
                     /locus_tag="POA77_001394"
     CDS             complement(18828..19610)
                     /gene="hisJ"
                     /locus_tag="POA77_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDI6364855.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19831..20613)
                     /gene="argT"
                     /locus_tag="POA77_001395"
     CDS             complement(19831..20613)
                     /gene="argT"
                     /locus_tag="POA77_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDI6364856.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20879..21448)
                     /gene="ubiX"
                     /locus_tag="POA77_001396"
     CDS             complement(20879..21448)
                     /gene="ubiX"
                     /locus_tag="POA77_001396"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDI6364857.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADIETHLVMSQAARQTL
                     SLETGFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21543..23060)
                     /gene="purF"
                     /locus_tag="POA77_001397"
     CDS             complement(21543..23060)
                     /gene="purF"
                     /locus_tag="POA77_001397"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI6364858.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLKDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23097..23585)
                     /gene="cvpA"
                     /locus_tag="POA77_001398"
     CDS             complement(23097..23585)
                     /gene="cvpA"
                     /locus_tag="POA77_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDI6364859.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23844..24506)
                     /gene="dedD"
                     /locus_tag="POA77_001399"
     CDS             complement(23844..24506)
                     /gene="dedD"
                     /locus_tag="POA77_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDI6364860.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24496..25764)
                     /gene="folC"
                     /locus_tag="POA77_001400"
     CDS             complement(24496..25764)
                     /gene="folC"
                     /locus_tag="POA77_001400"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI6364861.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25834..26748)
                     /gene="accD"
                     /locus_tag="POA77_001401"
     CDS             complement(25834..26748)
                     /gene="accD"
                     /locus_tag="POA77_001401"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDI6364862.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26904..27563)
                     /locus_tag="POA77_001402"
     CDS             complement(26904..27563)
                     /locus_tag="POA77_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6364863.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27646..28458)
                     /gene="truA"
                     /locus_tag="POA77_001403"
     CDS             complement(27646..28458)
                     /gene="truA"
                     /locus_tag="POA77_001403"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI6364864.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28458..29471)
                     /locus_tag="POA77_001404"
     CDS             complement(28458..29471)
                     /locus_tag="POA77_001404"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6364865.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29537..30673)
                     /gene="pdxB"
                     /locus_tag="POA77_001405"
     CDS             complement(29537..30673)
                     /gene="pdxB"
                     /locus_tag="POA77_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDI6364866.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30772..31767
                     /gene="flk"
                     /locus_tag="POA77_001406"
     CDS             30772..31767
                     /gene="flk"
                     /locus_tag="POA77_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDI6364867.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31764..32942)
                     /locus_tag="POA77_001407"
     CDS             complement(31764..32942)
                     /locus_tag="POA77_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364868.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRFILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33235..34455)
                     /gene="fabB"
                     /locus_tag="POA77_001408"
     CDS             complement(33235..34455)
                     /gene="fabB"
                     /locus_tag="POA77_001408"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDI6364869.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34614..36620
                     /gene="mnmC"
                     /locus_tag="POA77_001409"
     CDS             34614..36620
                     /gene="mnmC"
                     /locus_tag="POA77_001409"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDI6364870.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36741..37019)
                     /locus_tag="POA77_001410"
     CDS             complement(36741..37019)
                     /locus_tag="POA77_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDI6364871.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37053..37601)
                     /gene="epmC"
                     /locus_tag="POA77_001411"
     CDS             complement(37053..37601)
                     /gene="epmC"
                     /locus_tag="POA77_001411"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDI6364872.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37601..38410)
                     /locus_tag="POA77_001412"
     CDS             complement(37601..38410)
                     /locus_tag="POA77_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6364873.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLSEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38410..39234)
                     /gene="mepA"
                     /locus_tag="POA77_001413"
     CDS             complement(38410..39234)
                     /gene="mepA"
                     /locus_tag="POA77_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDI6364874.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39238..40323)
                     /gene="aroC"
                     /locus_tag="POA77_001414"
     CDS             complement(39238..40323)
                     /gene="aroC"
                     /locus_tag="POA77_001414"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI6364875.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40358..41290)
                     /gene="prmB"
                     /locus_tag="POA77_001415"
     CDS             complement(40358..41290)
                     /gene="prmB"
                     /locus_tag="POA77_001415"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6364876.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41456..42007
                     /gene="smrB"
                     /locus_tag="POA77_001416"
     CDS             41456..42007
                     /gene="smrB"
                     /locus_tag="POA77_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDI6364877.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
BASE COUNT        10385 a        11436 c        10523 g         9743 t
ORIGIN      
        1 agtttcacac cttgccccca ttacgacacc tcggaaatca tcacgctgtt aaactctccg
       61 taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg cattttcaca
      121 caattatctt acagataaaa aaaccagact tacaattaag aatcagaaca attcactata
      181 taacattgca tgtaaagcat atacacctaa ttattttgtc attattaagt tattaacagc
      241 acaatcgagc cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg
      301 ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag
      361 gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat
      421 ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa
      481 actgaacatc ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga
      541 cgtgatacgc aacctgccta ccgctcaagg gtattgcgat tccaaaggtc tttactccgc
      601 gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga
      661 tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa
      721 cagcggggac gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc
      781 gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga
      841 cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc
      901 aaataaccca accggcgcgg tatattccaa agagctttta atggagattg tggagattgc
      961 acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc tttacgacga
     1021 cgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg
     1081 actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc
     1141 gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg
     1201 tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag
     1261 tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat
     1321 caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa
     1381 aatcgacgcc aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt
     1441 gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt
     1501 ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg
     1561 tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc
     1621 agcgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag
     1681 agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc gctcatgcgc
     1741 aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg
     1801 ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta
     1861 ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa
     1921 tacttcaatt cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa
     1981 ctggtcgata tggttccgga agagctgcgg gatatctttg cgccgttaat tgacgagcat
     2041 gcatatagcg atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg
     2101 aaatgtctgg aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg
     2161 gaagcgacgc ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt
     2221 cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga
     2281 gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa
     2341 acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca
     2401 acttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg
     2461 aagaaaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat
     2521 aaggcgacac ccccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg
     2581 tcgcggtatt agagataaat agcccaataa ccgccgacaa gacaaacaaa caccccagca
     2641 tcatatgtgg cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac
     2701 ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg
     2761 gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca
     2821 ggcaggcgat gatagcggca acaggattag gaatttcatc tgtcagcatt aacgccacca
     2881 ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca
     2941 cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca
     3001 gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg
     3061 aaccttccag cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac
     3121 gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct
     3181 ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct
     3241 ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa
     3301 gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa
     3361 aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat
     3421 catccagccg ttgacctatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc
     3481 cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct
     3541 gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca
     3601 gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt
     3661 cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc
     3721 cgctaatcag cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac
     3781 gcatggcaac gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa
     3841 acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc
     3901 ccgccacttt gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat
     3961 cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata
     4021 cttcggggac cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg
     4081 catccatacg cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa
     4141 cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct
     4201 gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat
     4261 aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc
     4321 gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc
     4381 cgccaggcca gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc
     4441 aagccccagc agctgcgcgc ctaacagata cgcatcaggt tctggttttc cgcgcttcac
     4501 tcgctcagcg gttacaaaca cctctggtgc gggaagccca gctattttat ggcgcgctcg
     4561 cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg
     4621 attgagtaag gcgattgccc ccggaagcgc ggtaatacct tcggtttccg tggcctcgat
     4681 gtgctccaga cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg
     4741 gcgcaaagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa
     4801 cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag
     4861 cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga
     4921 taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat
     4981 taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaaccg tgagttccgg
     5041 cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac
     5101 gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct
     5161 ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata atgtcgatga
     5221 tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta
     5281 atccgtaacc acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat
     5341 cgagcatagt catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt
     5401 ccattgttga ctcctgtatc actatactac ggtgaaaaaa aagaaggctg agtatgcctt
     5461 cttttatatg cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt
     5521 ttgaaggcgc gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt
     5581 gccaacacct gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt
     5641 gcagggggta atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa
     5701 cttaaggcga acagcgcagt ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc
     5761 cagcagagag taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg
     5821 ataacgcgat tttcgacaaa gaccggggca aggcgttttt ccagcggcca cgtctttgag
     5881 taatgctgtc cccggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac
     5941 atgcttcacc tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc
     6001 gggacaacgt tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct
     6061 gaaggcctaa gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc
     6121 tgaaaattac gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt
     6181 gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc
     6241 tgacgttttt ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg
     6301 gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa
     6361 gagtaccttt ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa
     6421 atggacggca ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc
     6481 aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct
     6541 atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag
     6601 tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac
     6661 ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct
     6721 gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg
     6781 tacaacctgt acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc
     6841 tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc
     6901 acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac
     6961 acctctatgg gcctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc
     7021 gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac
     7081 aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc
     7141 tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc
     7201 caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct
     7261 gttgtattca ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc
     7321 aaactgggcg tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc
     7381 aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa
     7441 gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca
     7501 gctggcggtg ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt
     7561 cccgtattat tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg
     7621 gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc
     7681 agccgcgtac cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt
     7741 ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca
     7801 atcagaaaga tgtactgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg
     7861 ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc
     7921 tgaactacga aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca
     7981 ctgacacccc ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggcggtg
     8041 ccaaaaacac caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac
     8101 aggggcgtac tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa
     8161 acaatgttga tccggcgaag ctgcaagaat ccagcccgct gccggttctc ggcgctgtgc
     8221 cgtggagctt tgacctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga
     8281 ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac
     8341 gcagcattcc gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag
     8401 accgtcctga cgtgctggtg gccgcttgcc tggcagccat gaacggtgta gaaatcggtg
     8461 ccctgctgct gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg
     8521 ctttcgctac cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga
     8581 gcctgcagag cttcaacctg gaagttccgg ttgacgatca cgaacgtatc gagaaagttc
     8641 aggaatacgt tgctaactac atcaacgctg actggatcga atctctgact gccacttctg
     8701 agcgcagccg tcgtctgtct ccgcctgcgt tccgttatca gctgactgaa cttgcgcgca
     8761 aagcgggcaa acgtatcgta ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg
     8821 ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca
     8881 accgtgttgc agcgtctcag ggtgtagaac tgggtgcagg gattgaaatc gttgatccag
     8941 aagtggttcg cgaaagctat gttggtcgtc tggtcgaact gcgtaagaac aaaggcatga
     9001 ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg
     9061 aacaggatga agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc
     9121 gtccgccgct gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct
     9181 tcatgctgct gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga
     9241 ccgctgaaca gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta
     9301 tcgaaccgcg cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg
     9361 tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg
     9421 acggtccgct gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga
     9481 actctccggt tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca
     9541 ccacctacaa agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg
     9601 gtatgcgcaa gccggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca
     9661 ccatcgcgct gactgcgatt cagtctgcac agcagcagta atctcgtcat catccgcagc
     9721 tttgcgctgc ggatatctga accggaaata atcactattt ccggtttttt attctcttaa
     9781 tctgcattaa tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat
     9841 ttcactttgc atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa
     9901 tcactgaatc caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt
     9961 ttgttgctat tttaaccagc cttgccacct gggtagttcc ggtggggatg tttgacagtc
    10021 aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact
    10081 catttcgcat tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca
    10141 cgacgggcga tgaacgcccg ggcctgatga acttcccgtt tgaaggatta acctcaggat
    10201 cgaaatacgg gacagctgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg
    10261 gcattgtgat gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc
    10321 gcgggaatga aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg
    10381 tatttggtat gggagaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc
    10441 ggctgggcta tgacagtatt accaccgtcc tggtgaccta tattgccacg caaatcggtt
    10501 ttgccagttc gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt gccggcgttc
    10561 cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt tatcgccact atgattggcc
    10621 tgatctttac catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc
    10681 atgagtccga ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct
    10741 ttggtgactg gctggtattg attgtcctga ccgccgtaat ggtctgggtg atttggggcg
    10801 tgatcgttaa tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg
    10861 tgattggcat catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt
    10921 catcctttac cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca
    10981 aagggatttt gctgctggtc ggtaatggtg aagcgggtga tgccagcgtg ttaaatacca
    11041 tcctcaacag cattgccaat gccattagcg gtctggacaa cgcggtcgcg gcctggttta
    11101 tgttgctctt ccaggcggta tttaatttct tcgtgacgtc cggttctggt caggcggcgt
    11161 taaccatgcc gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg
    11221 tgctggcatt ccagtttggt gatggcttca gtcacatcat ttacccaacc tcagcttcgt
    11281 taatggcaac tctcggtgtg tgcagggtgg acttccgtaa ctggctgaag gtgggcgcga
    11341 ccctacttgg actgctgttt attatgtcca gcgtcgtagt gatcggcgct cagttgatgg
    11401 gctaccacta aaaatgttca gagccgcaat tgcggctctt ttttcattct gccgtttcag
    11461 tctctacggc ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt
    11521 ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaacttcac
    11581 tgacttcatc ttcctgtagg gcgaagggac cgtgagagac gcagctgaac aacgcgcccc
    11641 agacacggca atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg
    11701 caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct
    11761 gaaccacacc gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac
    11821 gacgctggac cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac
    11881 gcagacactg tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat
    11941 tgacaatatc cacccattca gtacttgcca aacgacgctg ttccattatc aagaaacctt
    12001 ctttttctgg cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga
    12061 cctgcgcaat gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca
    12121 tgccataact cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaaatgga
    12181 aaatctcccc ccgctgttct gccactggca gatgggtatg accgtacacc agcacatcgt
    12241 tctggtttaa agcaggtaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac
    12301 gttgtttttc cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcagcattt
    12361 gatccacttc gctgtcgcag ttgccgcgca cagcgataac cttatgtgcc acttcattaa
    12421 gccgttcagc gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat
    12481 tcaacacgtc gccaaggatc accagccact gggcaccgct ttgggcaaac aactccagaa
    12541 cacgttccgt cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc
    12601 actcctcgtc gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat
    12661 tgcttcgccg aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca
    12721 taatccacca ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc
    12781 gccagatcag tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtgctaac
    12841 aaatgttctg ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc
    12901 gcttttttcg cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga
    12961 tcgctgcgca gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga
    13021 taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactcctc aatggcagaa
    13081 gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccataa
    13141 ccttgccacg tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac
    13201 gacaggcctt tttcctgcaa cgccacccag gcggataaca cataagggga gaaaaagtgg
    13261 gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct
    13321 ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc
    13381 cgcgctttct atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc
    13441 tctatttcgc gccgacaccc aatggtcaca aaattacgct gtttctcgaa gaagcagagc
    13501 ttgattatcg cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt ccggaatttt
    13561 tgcgcatttc gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg
    13621 gcgaaccgct aagcctcttt gagtctggtg ccattttgtt gtatctggct gagaaaacag
    13681 gactcttttt gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc
    13741 aggtaggcgg actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc
    13801 aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac
    13861 tgaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata
    13921 ttgcctgctg gccgtgggtt aatgcctgga ctcgccagcg aattgacctc gcaatgtatc
    13981 cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc
    14041 taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt
    14101 atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg
    14161 tataaagaac cttcgtttgc gtacgtttat cggaattaag gaagaagaaa ttaacaaccg
    14221 tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga
    14281 agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga
    14341 gaataaccgt ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga
    14401 acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc
    14461 cgattcggta tccatgacct taagctggca gcgttaaccg ccatatcggg aggctgcatg
    14521 aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg
    14581 gagctgggcc atcaaatcac ggtagtgacg cgtaacccac agaaagccag ttccgttctc
    14641 ggccctcggg tgacgctatg gcaagggcta gccgatcaaa gcaacctcaa cggcgttgat
    14701 gcggtaatca acctggccgg agaaccgatt gctgataaac gctggactca cgagcaaaaa
    14761 gagcgtctct gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgcc
    14821 agcgacacgc caccgtcggt actcatttcc ggctcagcaa cgggctatta tggcgactta
    14881 ggtgaagtgg tggttaccga agaggaaccg ccgcataacg aatttaccca taaactctgc
    14941 gcccgctggg aagaaattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg
    15001 cgtaccggcg tagtgctggc accggatggc ggtattctcg gtaaaatgct gccgccgttt
    15061 cgtcttggcc tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatc
    15121 gatgatatgg tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat
    15181 atggtttcgc cctacccgat acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg
    15241 catcgcccgg ctattttgcg cgtccccgca accgccattc ggctgttaat gggcgaatct
    15301 tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt
    15361 gcgtttcgtt ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta
    15421 ctgcaaacat ccgccagtta actcccggtg ttacagggtt agtggctttg cgctataaga
    15481 tcgtctggcg atagtcgggt caccatcata actaactctc tgtctaaacc tctatccagc
    15541 atctcctgag caatacgcag ggcttcttcg cgtttgccca gaattaatcc atcattccgc
    15601 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    15661 tctgctatct caccaataaa tgcacggaaa cgctgggcat ctcccgtttg taatacataa
    15721 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    15781 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    15841 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    15901 aaagccgatg aatatatttt acgggcaatc gcaggctcag caaattcatc aagccagcag
    15961 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    16021 agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata acgcatcatg
    16081 cgaaaagcca ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc
    16141 actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcgtca
    16201 tcaataaaac tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc
    16261 gcgggaagat gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta
    16321 aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca
    16381 aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc
    16441 tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag
    16501 ctgactcgca aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta
    16561 tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg
    16621 ctccggcgcg ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac
    16681 atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag
    16741 ttgctgcata atacgtaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc
    16801 atcaaacagt aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg
    16861 ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt
    16921 tgccagatac ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat
    16981 cggcgcttcc atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa
    17041 taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg
    17101 accgtctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact
    17161 cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc
    17221 gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcatg
    17281 ttcgccgtag cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc
    17341 agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc
    17401 tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag
    17461 gttgatacgt ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg
    17521 taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg
    17581 caatacgcag cgcagaaggc aaaataatgc aacgatacat tttaaaagtc gagaagccat
    17641 aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct
    17701 cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc
    17761 ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat
    17821 agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga
    17881 ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga
    17941 ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc
    18001 gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc
    18061 agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac
    18121 agtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt
    18181 acttttgcct gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag
    18241 tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat
    18301 catcgccgga aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc
    18361 cgcctctata tgccctttcg gcactgccat aaacgcgcca cggaacgttt cggtaaaata
    18421 agcaccgtag ataaaaccga gagtaataat accagcgacc atcggatcga tatcaatctg
    18481 cccgacgccc atcgcctccg tcaccgtgtt tagtgcaatc tgcaaaccgt agaaaatcag
    18541 cagcatcaac accaagtccg gcacgcctcg aatcagcgtg gtgtaccctt cgaaaatcag
    18601 ccccgaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc
    18661 gagcactaca gaactgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc
    18721 tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc
    18781 tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta gccaccataa
    18841 acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg
    18901 gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg
    18961 gtccctacgc caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta
    19021 ccgacaggtt gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca
    19081 atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca
    19141 atgccttttg gtgcccaatg ttcgttaccg aacgtctcct gggtggtgcc ctgcaatacg
    19201 cctacccgct tgcctttcag cgactcgact gtcggctgaa tgtcggaatt tttcgccacc
    19261 accagacgag aatcggcagc gtacagtttg tcggtgaagg caatttcttg ctgacgtttt
    19321 tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt
    19381 aacgcatcca gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc
    19441 tttgccagat cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc
    19501 gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa cgccgcagtt
    19561 gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca
    19621 actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat
    19681 tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt atttttcatt aaaaatgctt
    19741 aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca
    19801 ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata
    19861 ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa
    19921 ggcagccgtc agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta
    19981 ttttttgtct tttactgatg agccagcaaa ggcgaaatct ttaccagcag gttgcttgag
    20041 gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag
    20101 atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca
    20161 ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt
    20221 cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc
    20281 ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga
    20341 cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact
    20401 agcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc
    20461 aaagccaaca aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt
    20521 tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac
    20581 taacaaagac agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt
    20641 tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct
    20701 tgacgacaac agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg
    20761 aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca
    20821 aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt
    20881 atgcgccctg ccagcgggca aagagatctt caggaagggt tatcgcaaac tggtcaagaa
    20941 cacgattaac cgtctgattt atcacatcat caagggactg tgggcgatga taaaacgccg
    21001 gaacgggagg cataatcacc gcaccgattt ctgctgcctg agtcattaaa cgcagatggc
    21061 ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca
    21121 catctgccgc acgggtcagt aaaccatcgg tgtagctatg gacaatgccg gaaagggttt
    21181 tgattgaaca gggtaaaata accatcccca gcgtctggaa agaaccggaa gagatgctgg
    21241 cggcaatatc gcgcgcatcg tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa
    21301 aacccgtttc gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga
    21361 tatctgcgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac
    21421 cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac
    21481 tttgcaggat ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg
    21541 catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg
    21601 gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg
    21661 acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga
    21721 acggcgtcga tcagatcttt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg
    21781 cgaatttcat caacttcgcg accgtgagcg ataagctccg tggcgctcgg catatcaata
    21841 ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct
    21901 ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg
    21961 tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc
    22021 acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt
    22081 ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca
    22141 cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca
    22201 attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg
    22261 atgaacgagt ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga
    22321 ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc
    22381 ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg
    22441 accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc
    22501 ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatac
    22561 gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc
    22621 gggtagtggc ggaaattgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg
    22681 gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg
    22741 ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc
    22801 tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata
    22861 ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct
    22921 ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg
    22981 cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg
    23041 ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac
    23101 gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa
    23161 ctgaattgcg ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag
    23221 gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag
    23281 acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg
    23341 aagttcacga tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaatccca
    23401 tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga
    23461 ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg
    23521 cggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag
    23581 accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa
    23641 aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa
    23701 atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg
    23761 gcgtaaacgc cttatccgac ctacggttct aacactgccc aggcctgata agacgcgcca
    23821 gcgtcgcatc aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag
    23881 ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc
    23941 ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga
    24001 taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca
    24061 cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc
    24121 ggttctggct tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc
    24181 ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata
    24241 gtggtcggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct
    24301 tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga
    24361 tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc
    24421 ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact
    24481 aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg
    24541 tgcgacagtg tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat
    24601 tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag
    24661 cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac
    24721 cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac
    24781 cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc
    24841 atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg
    24901 tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc
    24961 gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg
    25021 cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata
    25081 gttccactca acgccccgac gttgtaacag tgcacctttt tcctgtgcca catcagcaat
    25141 ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgga agatgcctgc
    25201 tttctcgcga ccaatacttt cgcgatctgg acccagccag tcggtatgat ccagcgcaat
    25261 actggttact accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag
    25321 ccctacttcc agaatcacca cgtcaagttg tgcctgcttg aacagccaca acgccgacag
    25381 cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa
    25441 agaggcggtg tgggccgatt ccggcaattc ctggccctgc acacgtacgc gctcggtata
    25501 acgcaccaga tgcggtgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga
    25561 ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg
    25621 cgctggtttc agaacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc
    25681 gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc
    25741 ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa
    25801 gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac
    25861 cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa
    25921 cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat
    25981 ggcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac
    26041 ggtctgttcg ataacacgcg gaccggcaaa gccaattaac gctttcggtt cagcgatgtt
    26101 gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac
    26161 ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat
    26221 ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat
    26281 cagcgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg caccaacaac
    26341 agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc
    26401 atacagagtg cctttcatca ccaccagcgc atctttttcg ccagtttctt tctgcgcaga
    26461 tgccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag
    26521 ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc
    26581 tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc
    26641 gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc
    26701 cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc
    26761 tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc
    26821 ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag
    26881 tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacga
    26941 ataaattcga aaaccccagg caatattgaa acaaaaataa tgccaacaat catcagctta
    27001 aggttgctct gaatgaaggg gagcgtgccg aagaaataac ccgcgtaggt aaaaatcagt
    27061 acccacaaca gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc
    27121 attcccgcaa caaacggcgc gaacgttctg acgatcggta caaaacgggc gagaataatc
    27181 gttttgccgc catgtctctc gtaaaactga tgagtcttat caagataact gcgacgaaag
    27241 attttcgaat ccgggttact gaacagtttg tcaccaaata accgtccaat ggtatagttc
    27301 actgcatcac cgacaatagc ggcaatcaac ataagcacca ccatcatatg gacattgaga
    27361 tcgttagttt ccagcgacgc caatgcacca gcaacaaaca gcagcgaatc accaggcagg
    27421 aatggcgtta ccaccagccc cgtttcgcag aacagaatca aaaacagaat ggcataaacc
    27481 cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg
    27541 atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg
    27601 taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa
    27661 tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag
    27721 atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa
    27781 ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat
    27841 attcctgacc atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc
    27901 gtgacgcgtg acattaatat gcataacgtt acgccacggg gttcgggact ggcactgcac
    27961 cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg
    28021 ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag
    28081 ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa
    28141 atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac
    28201 gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca ccacttgccc
    28261 ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc
    28321 cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg
    28381 ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag
    28441 ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact
    28501 gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc
    28561 ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc
    28621 cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac
    28681 gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca
    28741 tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc
    28801 aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc
    28861 ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc
    28921 ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc
    28981 tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt
    29041 aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc
    29101 cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg
    29161 ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct
    29221 tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc
    29281 cagtcgaatt cagcggcatc ctgcacggtg attgttttac caccaaagcg cagttgttcg
    29341 cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc
    29401 agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag
    29461 ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga
    29521 gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca
    29581 gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct
    29641 caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg
    29701 gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat
    29761 cgagcgggcc atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg
    29821 caacgtgctg ttcatgccca ataaacttgc tataggcttc aaacacttgc gtagtaccgc
    29881 gtgctttacc ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca
    29941 gcaactccac gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct
    30001 ggccttcatt caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt
    30061 taatcagaat cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg
    30121 ttttgtacgg cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa
    30181 ctaattcatc cagcgagcgg aaatcaccct catccccacg gtcagcgcga ggcggatcgc
    30241 aaagcaatgt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac
    30301 caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca
    30361 tcagtagtga ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg
    30421 aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg
    30481 cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg
    30541 aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac ggggattgga cgcccgggaa
    30601 ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa
    30661 caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca
    30721 taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa
    30781 cctatttccg gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc
    30841 gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa
    30901 gtgacctcac tgacgcaaca gcaaagagca gaactgtggg cgggtatcag gcacgatatt
    30961 ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg
    31021 gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta
    31081 ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac
    31141 tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg
    31201 ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac cgaccgtcct
    31261 ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg
    31321 ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt gaaagatggc
    31381 gagttaattc cagcgaaact gtttaaccat ctggtgacct ggctacaggc gcgtcagacg
    31441 ctaagccagc aaaatacgcc gacgctggaa tcactacaga tgacgctaaa acaaccttta
    31501 gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg tctttctgcg
    31561 caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg
    31621 gttaaaggga ttgatccgcg tgttatgcaa ccgctgctta atccttttcc accgatgatg
    31681 gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc
    31741 ctgatgctgg tctggctggt tcgttaaccc cgacgaaatg acagtatcgt gacaataata
    31801 cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc
    31861 agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg
    31921 cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca
    31981 ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca
    32041 cctggggctt gccagagcaa caacaagccc accgtttcta caagcagaga gacaatcgcc
    32101 actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat
    32161 gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag
    32221 agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct
    32281 ggtttccaga taaacccgac aacgctccac agcgatggac gttctcccgc cagggccggt
    32341 actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc
    32401 gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggagc accaacagcg
    32461 agggcaccgt aaatcgccat tccattccat gacatcactt tgccagagtg ttttgcccct
    32521 acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc aaacccaaga
    32581 ataaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg caaaatcgcc
    32641 gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt
    32701 tttgcgccat attgatcggc cagtcgcccg gcatagccac gcgtcagcac cgtagccaga
    32761 aactgaatcc cgacggcaat cccgaccatg gtattgccat agcccagatc atgatgaaca
    32821 aacagcggga taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa
    32881 gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc
    32941 atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt
    33001 cagggttaca gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa
    33061 agcgggaagt ttgcctggaa caggcggcga ttgtcaatga tgtgaaaaag ggaaccatca
    33121 ggttcccttt tgcgttagtg ccggaggccg cattagcgcg taacgtcgga tgcgacgctg
    33181 gcgcgtctta tccgacctac tccgactact ccgactactc cggcctactg cgaattaatc
    33241 tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat
    33301 aacggtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacctg cagcctgctc
    33361 gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga
    33421 gtagatagct tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc
    33481 agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagtt ctttcacgtc
    33541 gccaaccgga gtcgaagtac catgagagtt caggtaatcg attggggtat caacgccgtg
    33601 catcgccatc ttcatgcagc gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc
    33661 atcagaggtt gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc
    33721 gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg
    33781 gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc
    33841 acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa
    33901 cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga
    33961 agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag
    34021 gcaggcagaa acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag
    34081 gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc
    34141 agaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag aaaggcccgc
    34201 atctgcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg
    34261 cacaactttg cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct
    34321 acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag
    34381 agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat
    34441 cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca
    34501 atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga aatttgcgca
    34561 gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac
    34621 actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag
    34681 attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc
    34741 tgggaggcaa ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtggtag
    34801 cagagagcgg cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt
    34861 ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat
    34921 ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt
    34981 gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc
    35041 tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc
    35101 aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag
    35161 cgaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc
    35221 cgggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggacg
    35281 ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgcgggg
    35341 tgatggaaca gacattaccg ctcccctgct ccgcgccgtg gtttaaccgc acgggcagca
    35401 gcaaacggga agcggcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgc
    35461 tattacggcg cggctggcag gtaacgcttt attgcgcgga tgaggccccc gcactgggtg
    35521 cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa
    35581 accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta
    35641 aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata
    35701 aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa
    35761 atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag
    35821 gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg
    35881 gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt
    35941 tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc
    36001 atcaaatcag ccgattcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg
    36061 tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg
    36121 gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc
    36181 gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga
    36241 ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc
    36301 gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt
    36361 atgaggcaac actcgtggaa tatgcgtcgt tggcggagca gaaagatgag gcggtaagcg
    36421 cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg
    36481 ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg
    36541 ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta
    36601 aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct
    36661 tatccgacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat
    36721 ggtgctccag acgccgcaac ttactgctgt gacgcctgct gaaacaggtt ttcccacata
    36781 tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga
    36841 ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct
    36901 gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg
    36961 gcatggtcaa ccataccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc
    37021 ttctttcctc attgtaaaac gtggcgctgg ctttagttga gcgcttccgg ccacgggaac
    37081 tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc
    37141 gggcgttcag gaatgccgtt tgctaggtaa tccatcacct gcgcatgtac ccgccgttga
    37201 aacaccacac gatctggttc gaagtcgcct tccagattgt cgcaactgac gttaaacgga
    37261 tatcccgccg ccacacagaa caaccagtcg agtgcctgcg gcttcacttc aacatcttca
    37321 aactggcttt gcgtttgggc atcacgccca tccgggcaat accagtagcc gaaatcaacc
    37381 agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca
    37441 taaaagccgt gggcaaagac aattcggtta tacggtactt ccgcatcagc aggaagatag
    37501 atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta
    37561 aaaatttcaa tcaactgctc atagtggtgt gtactgttca ttagttcatc cccaaccagt
    37621 ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga
    37681 taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccagccgt gaacccatgc
    37741 gcgcgccgag gaactgcccc accagcatca caaagcccgt cgcccaaatc actttgccgc
    37801 cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag
    37861 ccgtggcttt ggcgaggttg aatccgcaca gcgtaacgaa ggccagcgcg taaaacgatc
    37921 cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga
    37981 agggtaaacc gtacatccgg cgctggcgat cttcttcact cagctttggc atcagcaaga
    38041 aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa
    38101 cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt
    38161 tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc
    38221 cgccgcaggc ttgcagttta ttggttgcca acgcgttagc gggagacatt cccgctgcca
    38281 tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca
    38341 gcatggcgac aaaaaagaga actcccagca acagcgggga aaccataaac aggctattaa
    38401 acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg
    38461 cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggtgcaaacc agctttgcag
    38521 ttctgccccg caaccatcgc ctggtggcgg taaaggttga tcttcacact ccagactatc
    38581 ggcaggacaa cgtaatcgta catgcatatg cgcacgatgc tggaaccagg gtcgcacttt
    38641 gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt
    38701 aacaaaaatg cgtgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc
    38761 tggcttccac agcgtcggaa caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg
    38821 cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac
    38881 atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag caggcatccc
    38941 catatcgcca atcagcaccg tacccatgcc cagattgctc acctgtctac tcagacgctg
    39001 gataaacatc accaggtccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg
    39061 ataatgttcg gactgtatcg gcagcgtgtc agcgccgacg atacagccat tagaaaaact
    39121 gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc
    39181 caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta
    39241 ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta gcaggtgatc
    39301 cattaaaacg atcgccagca tcgcttcagc gatcggcacc gcgcggatcc cgacacaggg
    39361 atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc
    39421 cggcacggta atgctggagg tcggtttcag cgccatatgg gcaatgattt gctgcccgct
    39481 gctgataccg ccgagaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc
    39541 gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc
    39601 tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac
    39661 cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga
    39721 gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tatccgggtc
    39781 cgggcagaag aacggatttt gttcaacctg cgaccagtct ttgatttcca gcggaatgtc
    39841 gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcagcga gatatttttt
    39901 ggcaatcgcc cctgccgcta cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc
    39961 gcgataatcg cgcagtccgt atttttgttc gtaggtgtaa tcggcatggc ctggacggaa
    40021 aacgtcctta atcgcactat aatcctgaga acgctggtcg gtattttcga tcaacaagcc
    40081 aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agaattttga cctgatccgg
    40141 ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacggtcga ggtcatgttg
    40201 caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc
    40261 cagcccatgc gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc
    40321 catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat
    40381 attcacgggc cgccagcagc tgctctttgg tgagcataaa cacaccatcg ccgccgttat
    40441 caaactccag ccaggtgaac ggaacatccg gatattgttc cataagatgt accatgctgt
    40501 tgccgacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg tttccaagaa
    40561 tgcgacgtgt cagtttaagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac
    40621 gatattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa
    40681 tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt
    40741 tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga
    40801 tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc
    40861 cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt
    40921 tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa
    40981 attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga
    41041 cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc
    41101 gcatatcttc cgggatatcc agcggcagat agagcgatgg taacaccagc tgtacggctt
    41161 catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta
    41221 ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa
    41281 ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg
    41341 aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat
    41401 cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa
    41461 aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac
    41521 tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt
    41581 gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt
    41641 tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt
    41701 tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg
    41761 tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga
    41821 acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac
    41881 accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta
    41941 cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gagtggttgc cgccggagtt
    42001 gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata
    42061 tgtgataatt caataaattg caattca
//
LOCUS       DAMZII010000027        41450 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZII010000027 DAMZII010000000
VERSION     DAMZII010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.027"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(6..839)
                     /gene="fdhD"
                     /locus_tag="POA77_001417"
     CDS             complement(6..839)
                     /gene="fdhD"
                     /locus_tag="POA77_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI6364878.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1033..4083
                     /gene="fdnG"
                     /locus_tag="POA77_001418"
     CDS             1033..4083
                     /gene="fdnG"
                     /locus_tag="POA77_001418"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1618..1620,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI6364879.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVLEDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4096..4998
                     /gene="fdoH"
                     /locus_tag="POA77_001419"
     CDS             4096..4998
                     /gene="fdoH"
                     /locus_tag="POA77_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDI6364880.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            4995..5630
                     /gene="fdoI"
                     /locus_tag="POA77_001420"
     CDS             4995..5630
                     /gene="fdoI"
                     /locus_tag="POA77_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI6364881.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5627..6556
                     /gene="fdhE"
                     /locus_tag="POA77_001421"
     CDS             5627..6556
                     /gene="fdhE"
                     /locus_tag="POA77_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDI6364882.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSAINPFLFPGEGE"
     gene            complement(6886..7128)
                     /locus_tag="POA77_001422"
     CDS             complement(6886..7128)
                     /locus_tag="POA77_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364883.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7347..7565)
                     /locus_tag="POA77_001423"
     CDS             complement(7347..7565)
                     /locus_tag="POA77_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDI6364884.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8170..8460)
                     /locus_tag="POA77_001424"
     CDS             complement(8170..8460)
                     /locus_tag="POA77_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6364885.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8461..8772)
                     /locus_tag="POA77_001425"
     CDS             complement(8461..8772)
                     /locus_tag="POA77_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6364886.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            9001..9909
                     /locus_tag="POA77_001426"
     CDS             9001..9909
                     /locus_tag="POA77_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6364887.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(9973..10914)
                     /gene="fabY"
                     /locus_tag="POA77_001427"
                     /gene_synonym="yiiD"
     CDS             complement(9973..10914)
                     /gene="fabY"
                     /locus_tag="POA77_001427"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDI6364888.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10959..11396)
                     /gene="dtd"
                     /locus_tag="POA77_001428"
     CDS             complement(10959..11396)
                     /gene="dtd"
                     /locus_tag="POA77_001428"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI6364889.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11393..12265)
                     /locus_tag="POA77_001429"
     CDS             complement(11393..12265)
                     /locus_tag="POA77_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDI6364890.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12259..12858)
                     /gene="yihX"
                     /locus_tag="POA77_001430"
     CDS             complement(12259..12858)
                     /gene="yihX"
                     /locus_tag="POA77_001430"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDI6364891.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(12957..13742)
                     /locus_tag="POA77_001431"
     CDS             complement(12957..13742)
                     /locus_tag="POA77_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6364892.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(13776..14672)
                     /gene="yihV"
                     /locus_tag="POA77_001432"
     CDS             complement(13776..14672)
                     /gene="yihV"
                     /locus_tag="POA77_001432"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDI6364893.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            14840..15736
                     /gene="yihU"
                     /locus_tag="POA77_001433"
     CDS             14840..15736
                     /gene="yihU"
                     /locus_tag="POA77_001433"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDI6364894.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            15760..16638
                     /gene="yihT"
                     /locus_tag="POA77_001434"
     CDS             15760..16638
                     /gene="yihT"
                     /locus_tag="POA77_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDI6364895.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            16655..17896
                     /gene="yihS"
                     /locus_tag="POA77_001435"
     CDS             16655..17896
                     /gene="yihS"
                     /locus_tag="POA77_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDI6364896.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18034..18936
                     /locus_tag="POA77_001436"
     CDS             18034..18936
                     /locus_tag="POA77_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDI6364897.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19135..21171
                     /gene="yihQ"
                     /locus_tag="POA77_001437"
     CDS             19135..21171
                     /gene="yihQ"
                     /locus_tag="POA77_001437"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDI6364898.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            21217..22602
                     /locus_tag="POA77_001438"
     CDS             21217..22602
                     /locus_tag="POA77_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364899.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            22645..24048
                     /locus_tag="POA77_001439"
     CDS             22645..24048
                     /locus_tag="POA77_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364900.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            24116..24808
                     /gene="ompL"
                     /locus_tag="POA77_001440"
     CDS             24116..24808
                     /gene="ompL"
                     /locus_tag="POA77_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDI6364901.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYNFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(24899..26164)
                     /locus_tag="POA77_001441"
     CDS             complement(24899..26164)
                     /locus_tag="POA77_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364902.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(26266..27246)
                     /locus_tag="POA77_001442"
     CDS             complement(26266..27246)
                     /locus_tag="POA77_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDI6364903.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(27254..27964)
                     /locus_tag="POA77_001443"
     CDS             complement(27254..27964)
                     /locus_tag="POA77_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDI6364904.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSSVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(28181..30004)
                     /gene="typA"
                     /locus_tag="POA77_001444"
     CDS             complement(28181..30004)
                     /gene="typA"
                     /locus_tag="POA77_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDI6364905.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            30377..31786
                     /gene="glnA"
                     /locus_tag="POA77_001445"
     CDS             30377..31786
                     /gene="glnA"
                     /locus_tag="POA77_001445"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDI6364906.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            31960..33009
                     /gene="glnL"
                     /locus_tag="POA77_001446"
     CDS             31960..33009
                     /gene="glnL"
                     /locus_tag="POA77_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDI6364907.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            33021..34430
                     /gene="glnG"
                     /locus_tag="POA77_001447"
     CDS             33021..34430
                     /gene="glnG"
                     /locus_tag="POA77_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDI6364908.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            34542..34652
                     /locus_tag="POA77_001448"
     CDS             34542..34652
                     /locus_tag="POA77_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDI6364909.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(34842..36215)
                     /gene="hemN"
                     /locus_tag="POA77_001449"
     CDS             complement(34842..36215)
                     /gene="hemN"
                     /locus_tag="POA77_001449"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI6364910.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(36404..36913)
                     /gene="yihI"
                     /locus_tag="POA77_001450"
     CDS             complement(36404..36913)
                     /gene="yihI"
                     /locus_tag="POA77_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDI6364911.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            37495..38127
                     /locus_tag="POA77_001451"
     CDS             37495..38127
                     /locus_tag="POA77_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI6364912.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(38509..41295)
                     /gene="polA"
                     /locus_tag="POA77_001452"
     CDS             complement(38509..41295)
                     /gene="polA"
                     /locus_tag="POA77_001452"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI6364913.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT        10086 a        10784 c        10305 g        10275 t
ORIGIN      
        1 tttaattaat tgctcaaacg ctgcggatgg gtataaaccg ttgcccgacc cggcttacaa
       61 aaacccacca gcgtcagatt acaacgctca gccacttcta cagcaagcgt ggtcgcggca
      121 gacaccgcaa acaaaatctc tacgccgcac atcgccgact tttgcaccat ttcataactg
      181 gcacgactgg aaaccagtac cgcaccttgc tgccagcttt ccccttcttg tgaccggcgg
      241 cctaacagtt tgtccagcgc cacatggcga cccacgtctt catgcccgcc gaccagttcg
      301 ccagatggca acatccaggc agcggcgtga gtacaaccag tcagttgccc cactggctga
      361 aaatcgttga gatgacgtaa tgcatcatcc agtttgttga gatcaaacgt ctgggtgaac
      421 ggtagcggct gcaccggttt tccgatgtca ttaagttgct ccacgccgca tacgccacag
      481 cccgtacgtc ccgccagcgc ccggcggcgc tccttcaacc ccataaagcg gcggctggaa
      541 agctcaattt gtacttcaag accattacag gaaggaacga catccatgcc gaagatatcg
      601 cgcggacttt ctattatccc ctcggaaagc gaaaaaccga gcgcaaagta ctcaaggtct
      661 ttgggcgacg ccatcatcac cacatgcgaa atgccgttgt agaccagcgc aacgggaact
      721 tcttccgcga cctcatctaa ccgtgggtgt tgtaaatcat cacgccgcca taactcaatt
      781 tgacgaacac ctgtgatatt tgtcacattt tcgatttctt tttgctgtgt ttttttcact
      841 ttctcttaac cagataagaa cacacacacc aacattatgg tattctgtta caaacccttc
      901 ctggatggag ggaaattgag ccaattctgg acctttgcgg ccccttccgc aaagaaaaat
      961 aactcccact ccctgcacac gcagcaagcg aatgtaaatg ggacgtgaca atgtcgaaac
     1021 aaggagcaat ccatgcaggt cagcagaagg cagttcttta agatctgcgc tggcggtatg
     1081 gcaggcacca cggcagcggc actgggtttt gcacccagcg tagcgctcgc ggaaacccgg
     1141 cagtataaac tgctgcgcac ccgcgaaacc cgtaatacct gcacctattg ttccgtaggc
     1201 tgtgggctgt tgatgtacag cctcggtgac ggagcaaaaa acgccaaagc atctatcttc
     1261 catatcgaag gcgatccgga tcacccggtc aaccgcggtg cactttgtcc gaaaggcgct
     1321 ggcctggtgg atttcatcca ctccgaaagc cgtctgaagt ttccggaata ccgtgcgcca
     1381 ggttctgata aatggcagca aatcagttgg gaagaggcgt ttgatcgcat cgccaaactg
     1441 atgaaagaag accgcgatgc taactacatt gcgcaaaacg ccgaaggcgt gactgttaac
     1501 cgctggctct ccaccgggat gctgtgtgct tccgcgtcga gtaacgaaac cggctattta
     1561 acgcaaaaat tctcccgcgc gctgggtatg ctcgcggtcg acaaccaggc gcgtgtctga
     1621 cacggaccaa cggtagcaag tcttgctcca acatttggtc gcggtgcgat gaccaaccac
     1681 tgggtcgaca tcaagaacgc caacctcgtc gtggtgatgg gcggtaacgc cgctgaagct
     1741 cacccggtcg ggttccgctg ggcgatggaa gccaaaattc acaatggcgc gaagctgatt
     1801 gtgatcgatc ctcgctttac gcgtacggcg gcggtggctg actactatgc ccctattcgt
     1861 tccggtactg acattgcttt cctgtcaggc gtattgctgt acctgctgaa caatgaaaaa
     1921 ttcaaccgcg aatacaccga agcctatacc aacgccagcc tgatcgtgct tgaggattac
     1981 ggctttgaag atggcctgtt caccggctat gacgcggaaa aacgcaagta cgataaatcc
     2041 tcctggactt acgaactgga cgaaaacggc ttcgccaaac gcgataccac gctgcaacat
     2101 ccgcgctgcg tgtggaactt gctgaaacag cacgtttccc gctacacgcc agatgtggtt
     2161 gaaaacatct gtggtacgcc aaaagacgcg ttcctgaaag tctgcgaata catcgcagaa
     2221 accagtgctc acgataaaac tgcctcgttc ctgtatgccc tcggctggac gcaacactcc
     2281 gttggtgcgc aaaacattcg tacgatggcg atgatccagc tgctgctcgg caatatgggg
     2341 atggccggcg gcggcgttaa cgccctgcgc ggtcactcca atattcaggg gctgacggac
     2401 ctggggctac tgtcgcagag tctgccaggt tacatgacgc tgccaagcga gaagcagacc
     2461 gatctgcaaa cctaccttac cgccaacacg ccaaaaccgc tgctggaagg ccaggtaaac
     2521 tactggggca actacccgaa attcttcgtc tctatgatga aggccttctt tggtgataaa
     2581 gcgacggcgg aaaatagctg gggctttgac tggttgccga agtgggataa aggctacgac
     2641 gtcctgcagt acttcgagat gatgaaagag ggcaaggtca atggctatat ctgccagggc
     2701 tttaaccctg ttgcctcatt cccgaacaaa aacaaagtga tcggttgtct gtcgaaactg
     2761 aagttcctcg tcaccatcga cccactgaac actgaaacct ctaacttctg gcagaaccac
     2821 ggtgagctga acgaagttga ctcgtcgaag atccagaccg aagtgttccg tctgccatcg
     2881 acctgcttcg cagaagagaa cggttcaatc gttaactcag gtcgctggtt gcagtggcac
     2941 tggaaaggtg cggacgcccc ggggattgcg ctgactgatg gcgagatcct ctccggtatc
     3001 ttcctgcgct tgcgcaagat gtatgccgaa cagggtggcg cgaacccgga ccaggtgctg
     3061 aacatgacct ggaactacgc cattccgcat gagccgaagt cggaagaagt agcgatggag
     3121 agcaacggta aggcgctggc cgatattacc gatccggcaa ccggtgcggt tatcgtcaag
     3181 aaaggccaac aacttagctc gttcgcccaa ttgcgcgatg acggtacaac ctcctgtggc
     3241 tgctggattt tcgccggtag ctggacgccg gaaggcaacc agatggcgcg tcgtgataac
     3301 gccgatccgt ctggcctcgg taacacgctg ggctgggcat gggcatggcc gcttaaccgc
     3361 cgcattctgt ataaccgcgc ctccgcagat ccgcagggta acccgtggga tccgaagcgt
     3421 cagttgctga aatgggacgg cactaagtgg accggctggg atattccgga ctacagcgca
     3481 gcgcctccgg gtagcggcgt cgggccgttt atcatgcagc aggaaggcat ggggcgtctg
     3541 tttgccctcg ataagatggc ggaaggtccg ttcccggaac actacgagcc gtttgaaacg
     3601 ccgctgggaa ctaacccgct gcatccaaac gttatctcga acccggctgc acgtatcttt
     3661 aaagacgacg ccgaagcatt gggtaaagcc gataagttcc cgtatgtcgg aaccacctat
     3721 cgtctgaccg agcatttcca ctactggacc aaacacgcgc tgttgaacgc gattttgcaa
     3781 ccagagcagt ttgtggaaat cggggagtcg ctggcgaata aacttggcat tgcccagggc
     3841 gataccgtga aagtctcctc caaccgtggc tatatcaagg ccaaagcggt ggtgaccaaa
     3901 cgtattcgta cactgaaagc gaacggcaaa gatatcgata ccatcggtat tccgattcac
     3961 tggggctatg aaggcgtagc taaaaaaggc tttattgcca atacgttaac gccattcgtt
     4021 ggtgatgcga acacgcagac gccggagttt aagtccttcc ttgtgaatgt ggaaaaggtg
     4081 taacggagac gacatatggc ttatcaatct caagatatca ttcgtcgttc cgcgactaac
     4141 ggtctgaccc ccgcgcctca ggcgcgggac ttccaggaag aagtggcgaa actcatcgac
     4201 gtcaccacct gtatcggctg taaagcctgt caggtggcgt gttcagagtg gaacgacatt
     4261 cgcgataccg tcggcaataa cattggggtg tacgacaacc ccaatgattt aagcgccaaa
     4321 tcgtggacgg taatgcgctt ctcggaagtg gagcagaacg acaaactgga atggctgatc
     4381 cgcaaagacg gctgtatgca ctgttccgat ccaggctgcc tgaaagcgtg cccggcggaa
     4441 ggtgcaatta ttcagtatgc caacggtatc gtcgacttcc agtccgagca gtgcattggc
     4501 tgcggttatt gcattgcggg ctgtccgttc gacattccgc gcctcaaccc ggaagacaac
     4561 cgcgtctaca aatgtacgct gtgcgttgac cgcgtggtgg ttgggcaaga accagcctgc
     4621 gtgaagacct gcccaacggg cgcgattcac ttcggtacga aagagtcgat gaaaacgctg
     4681 gcgagcgagc gcgtggcgga gctgaaaacc cgcggttacg acaatgcggg cctgtacgat
     4741 ccggcaggcg tcggtggtac acacgttatg tacgtgctgc accatgctga caagccaaat
     4801 ctgtatcatg gcttgccgga gaacccggaa atcagcgaaa ccgtgaaatt ctggaaaggc
     4861 atctggaaac cgctcgcagc tgttggcttt gcggctacct tcgcagccag tatcttccac
     4921 tacgtgggtg tcggtccgaa ccgtgcggat gaggaagaga ataatctgca cgaagagaaa
     4981 gacgaggagc gcaaatgaaa cgacgtgaca ccatcgtgcg ctacaccgcg ccggaacgta
     5041 tcaaccactg gatcaccgcc ttctgcttca tcctggcggc agtgagcggg ctgggctttt
     5101 tgttcccgtc cttcaactgg ttgatgcaaa tcatgggcac accgcagctg gcgcgaattc
     5161 tgcacccgtt tgtcggcgtg gttatgtttg cctcgttcat catcatgttt ttccgttact
     5221 ggcatcacaa cctaatcaat cgggatgata tcttttgggc gaagaatatt cgtaagatcg
     5281 tcgtcaacga ggaagtaggt gacaccgggc gttataactt cggtcagaaa tgcgttttct
     5341 gggcggcgat tattttcctg gttctgctgc tggtgagcgg cgtgattatc tggcgtcctt
     5401 attttgcgcc tgctttctca atcccggtga tccgattcgc gttaatgctg cattcatttg
     5461 ccgcagtggc gttaattgtg gttatcatgg tgcatatcta cgccgccctt tgggtgaaag
     5521 gcacgattac cgccatggtg gaaggatggg ttaccagcgc ctgggcgaag aaacatcacc
     5581 cgcgctggta ccgtgaggtc cgcaagacaa cggaaaagaa agctgaatga gtattcgcat
     5641 aatcccgcaa gatgagctgg gttcgagcga gaaacgtacg gcggatatga ttccgccgtt
     5701 attgttccct cggctcaaga atttatacaa ccgccgcgcc gagcgtctgc gcgagctggc
     5761 agaaaataat ccgctgggtg attacctgcg ctttgctgcg cttatcgccc acgcccagga
     5821 agtggtgctg tacgaccatc cgctggagat ggatctgacc gcgcgcatta aagaagccag
     5881 cgcacagggc aagcccccgc tggatattca cgttctgccg cgtgataagc actggcaaaa
     5941 gctgctgatg gcgctgattg ctgagctgaa acctgaaatg agcggcccgg cgctggcagt
     6001 gattgagaat ctggagaagg catcaactca ggagctggaa gatatggcca gcgcactgtt
     6061 tgcctctgat ttctcatccg tcagcagcga taaagcgccg tttatctggg ctgcactgtc
     6121 gctctactgg gcgcagatgg ccaatctgat ccccggcaaa gcccgcgctg aatacggcga
     6181 gcaacgtcaa tattgcccgg tttgtggttc tatgccggtg tccagcatgg tgcaaattgg
     6241 caccactcag ggtctgcgtt acctgcactg caacctgtgt gaaaccgaat ggcacgtagt
     6301 gcgcgtaaaa tgcagcaact gcgagcagag cggcaaactg cattactggt cgctggatga
     6361 cgaacaggcc gcgattaaag ccgaaagctg cgatgactgt ggcacttacc tgaagattct
     6421 ttatcaggaa aaagaaccga aagttgaagc cgtggcagat gacctcgctt ctctggtact
     6481 ggacgcgcga atggagcaag aaggctatgc ccgcagtgcc atcaacccgt tcctgtttcc
     6541 gggtgaaggg gagtaatctc tgacgttttc cgggtggcgc tggtcgcccg gattacagct
     6601 catagggtgt gacgacacca tctctcgtat tccgcgttac ccattcaaga tcatgccgca
     6661 attgtctgtt tgcctcgttc ttccctccaa cacatcgata aggttatggg tatgtcactg
     6721 atgacaagcg tatgattgcc tgatgcgctg cgcttatcag ccctaccaat aaacttcaat
     6781 atattgaatt tgcatgactt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     6841 caacgcgcac gttgtcagca atctgattga catcattttt tcccattacc attcctcgcg
     6901 cagcttacgc tgatattcga caccatcttc ctcacaccct tgccagatgc caggaacact
     6961 ggttcttgct gtttgcgatt gatttatcag gtattgatct accgcttccc ttaataggtc
     7021 cgcacgagga agattacgct gcacctcgag atcatcaagt tgcttaatca cctcattcga
     7081 taaatcgagt aaaattctgc ccatatctat acctgccaga gagttcataa atatctcgcc
     7141 aatatcattt ttgaatctat ggagagaaaa gtacccttgc cgaatcttta aagaaagctc
     7201 atttacgcat cattttttat ttttgcgagg cgttttccag atacgatgta acagttgcag
     7261 ttattataag ttcaagtaaa acagccttgt attaatacca gaataagggc atctgttttt
     7321 ttatattcaa gaatgaaaat tttttttcat tccttatgct ccttacactg atcatcaaca
     7381 ccaactacct cccccttttg cctgcctccc tttgctttac gcaccggaaa ttgctgccgc
     7441 tccagatatt gctcaaccgc ttcgcgtaaa attgcagatc gtgaaacctt acgccagccc
     7501 cgaagtttgt tcagccgttt aattgcctct tctgataaat gaagaaaaac cgtattcatc
     7561 gccattacca ccaccttaca tatattactc attaatgtat gtgcgaaaat tacgccggaa
     7621 aatcatttaa aaaaagcgga tttttgcatg ttgttttgtt tttgcgaggt gttttccaga
     7681 aataataaga gtgttgcagt tgtttcattt attactcaaa aatggtttag ttatcaccca
     7741 ggatgagtta tggatgctat gaatatatta tattcaggga tgtttatttt ttgattggat
     7801 ctttatttgc ataatctgaa tggtgtaccc gaaaaatgtc agcatcctaa cagcacccgg
     7861 atttaaggtg aaattaatca tccatcacag accggtcagt actgccacca accggtcttt
     7921 acttgttcat cgttataatt ttgttggtaa cagttcggga aacaaggaat tccttccgga
     7981 accccccggt atcatccgat agcattgacg tcaatcatca gcgcgacgat gagttttttt
     8041 aatcgcatca tagggtggcg acgttcagca cgattacggg tcatagcaaa actcctgggc
     8101 aggcgaccac tatgccgctt tcacaggagt tctgaatgct agtgctttct gtatggcata
     8161 accatgcctc taacggttaa gtgcttgcag cacatgttgc ggatcattgg caatcgctcg
     8221 cagcaaagcc tttgcaggcc cggtcggtga acgtcgcccc tgctcccagt tacgcaacgt
     8281 gtctacgctt acagaaataa gatcggcaaa tttagattgt gacaaccctg aagcgcggcg
     8341 tatttctttg attttcatag catcaacttc aaaagtccgc gaaggctgtc gcgttccttc
     8401 aataatttca ttcatctggg ccatgctgtg agtcaaccgc tcaaaaagtg ccttatccat
     8461 ttaccacctc gtatttaaca accggagcat tgctttttcc tggggagaca gatcatcttt
     8521 aataccctta cgataaataa gcagtaatct gatctcataa tgtttaacct gatgaaagta
     8581 aataaccctg acaccagcac gtttcccctt tcctcctgaa acccatcgaa cctttcttaa
     8641 cccaccagtt tcaggtatta cctcaccata gtcaggattc aaggccagga aaaactgaaa
     8701 acgactgaat tcatcgtcgg tcagcagttt ttgaacatct tcagtaaaaa tttccgtttc
     8761 gatgaagagc ataattatac gccattggct tagagtaaca atcctttttg tacgtcattg
     8821 acgtaccctt gaaaccacta gcttactgat agtgctccag caaaaccacg tctctgccgg
     8881 gtttgtcttt agatttgcga ggcgctttcc agatttttca cgcccgtgaa tttccctgta
     8941 tccatccagc aaaaacgggt gtataagact gtacattcat acagcaaaat aagttctact
     9001 atggcactgg aaaaaggaat tgcagagtta gtcgaaaagt ttatcgctgc gggtcgcccc
     9061 tcatcgcgtc aacaaagtat tgcgcagcgc agggagggct atatcgccag cgctgtcctt
     9121 gcgggtgaga cagaaacgcg ggtagatatt cagactatcg aacttgaagg gatgacctta
     9181 cgaatagtgt cgccgctgaa tgccccgaca ttactcccga cgataattta ttactacggt
     9241 ggctgttttg ttagcggcgg gttcgcaact cacgacaacc agctgcgcca gttagcctat
     9301 tacgggcaat gtcgggttat tgccgtccag tatcggctgg caccggaaca tactttcccc
     9361 gccgcccatg atgatgcgca acgaggtgca gaaatagtct ggcaacacgc cgaacgactc
     9421 ggcgttgata aacaacgtat aactcttgcc ggtgacagtg cgggcggaca tctggcgctg
     9481 gtaactgctt tgcgattaaa gcgtgctggc gagtggcaac cggcacaact catcttaatt
     9541 tatcctatgc ttgatgcgac tgcgcatttc gaaagctaca tacgcaatgg tcacgattac
     9601 atcatcacac gcgacacttt actgagcggc tttgagatgt atctgccagg cattgaacgc
     9661 aggcatcctg aagccagccc tctttggcgc gatgatttca atggcttgcc acctgtacat
     9721 atcattaccg ctgaatacga cccactttgc gatgaaggtg aagcgttgta tcaccgcatg
     9781 acagggcaag gagtgcaatg cacctgccaa cgctaccttg gcgttatcca cggatttttt
     9841 cagttaggtg gtatcagcga ggccgcgcga tcggcactac gggatgttgc ctggagagcg
     9901 ggaaggtaac ttcggacctg ggccctgaaa aggttttcag attgaaaacc ttttcagtca
     9961 agtaaaacgc ccctactctt cttcgttccc gccctcttca tacgggccaa atggcttcgc
    10021 gggcagaacg atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc caaagatttc
    10081 aacttgcatc tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa
    10141 ggcaccaagg tcggctaccg catgaggttt accgctaatc ggcttgctgt agcggatatg
    10201 cgcatccgcc agaataatcg ttccgccgag atggcgttcg cgcagcatca gccagataag
    10261 cccccaaccg gtgagcgttg ccagtgagaa taaactcccg gcaaacagcg tatggtgcgg
    10321 attctgatta ccggtttccg gcatggtggt gataaatttt tgcccggtat attgttgaat
    10381 acgcacgccc attttttcac taagcgggat atgttcgtac cacgcctgtt gcagctgcgc
    10441 gcaccagtcg ccgcgatgca aaatatcatc cagagtggca acgggtttaa tcatcaaaaa
    10501 atggcgaatc ggcgtggtag ttggggtggt gatctccccc tgattaatga atcccagctt
    10561 ggcgaaaaac tccaccgcgt cttcacgggc gctacaggtc acgcgcttaa cgccttcctg
    10621 acgcgccacc gactccaggg tcatcgccat cagcgtgcct aaccctttgt cctgcacgtc
    10681 cgggtgaacg gccataaagc gaatggacgc ttcattgtcg gcattaatat acaatcggcc
    10741 taccgccacc agattaccct gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca
    10801 cgcgtcgcgt tccgaacctt ttggttgatg caggggctta cgcaacattt cccagcgaaa
    10861 ctgatagtaa cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat acatagcgat
    10921 actctctctt gtttcccgtg acaaccctgg aagctggctc atacctgcaa ccagaatgtc
    10981 acggggccat cattgaccag cgatacctgc atatccgcag cgaagcgtcc tgtttgcgtg
    11041 ttcatctctt gctgacggca gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc
    11101 ggtgatgcac ctttggagaa acttgggcgc atcccccgtt cggtatctgc ggcgagggta
    11161 aactgggaaa ccaccagcac actgccgccc gcctgttgca cgttgaggtt catcttgcct
    11221 tcggcatcgc taaagatgcg gtagccgagc acacgctcgc acagacggtt tgctttctgt
    11281 tcgtcgtcat ccttttcgac acccaataac accaaaagtc ccgcgccaat ttcgcccgtc
    11341 acttctccct ccacggtgac gctggcacgg gttacgcgtt gaattaatgc aatcatggtt
    11401 cgtcgtcttc ttcttgttca gctgcttgtt ttagtttgcg gtattccccg agagtgacag
    11461 taatttccgc gccaagcaag acgatacacc atgtccagta gacccaaaca aagagaatgg
    11521 ggatcaccgc cagcacgccg taaatgagct gatatgaggg gaacatggtg atataaagcg
    11581 cgaaaccttt ctttcctgct tcgaacagga gcgcggcgac aaacgcgccg acaatcgcgt
    11641 cgcggttagg tacgcggatg gtaggaacaa tgctgtacag caaccagaag gagatccacg
    11701 acaacagcag cggaaaaata cgcagcacgt tatcgatgac agtattgaga tcgctcgccc
    11761 agcgcaggga gagcaaatag gaactgatcg ccagactggc ccctgccagc agcggcccca
    11821 gcgttaaaat catccagtac acggcgaacg aataaatttt tggtcgcgct cgtttactgc
    11881 gccagatggt attcaacgcg ctatcgatgg agtacatcag caataacgcg gtgacgatca
    11941 gcccgcacgc cccaacggcg gtcatcttgt tggaattggc aacaaattgt tcgatatacc
    12001 gctgaataac atcgccagta gcaggcagaa agttggcaaa aataaagtga cgtaactgaa
    12061 tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg
    12121 gcactaatga gagcaacgac acataggcaa ggttacctgc cagggttgtc atgttgtcct
    12181 catcaatgcg ttgccagagt agttttagcc aggcccatag tggacgggta cggtgcctgg
    12241 ctttgtcctg aatggttttt agcataacac cttcgcgaaa tagtccggga tggtggtttt
    12301 atctttcacc agaatactgg taatgcccag ctgattggct ccttctatat tatcggcgtt
    12361 atcgtcgaaa aagaccgtat cgctgggtga aaaaccttcc gcctgcaaaa catgctggta
    12421 aattcgtgct tcaggtttgc gcatccccag atcttgcgac agatagatat ggtcagcagc
    12481 atcacgaatt tccgggtatt cttccggcca gaaggtggta tgcaggcggt tggtattgga
    12541 aagcaccacc acgcgatgcc cctgctcacg cagtttatgc atgatggcga tcacttccgg
    12601 gcgcagcgca acaaacaccg cctgccagcc gtgagagaac tgctcgtagc ttagcggtag
    12661 agccatctca tgacacagcg cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg
    12721 ctgatgaaac gcctccccca tatgaaaact cttcttaagc gatgccagcg gaatacgcgt
    12781 taaatcgctc caggctccca gcacacggtt aaagtcgata tcgacaatca cattacctaa
    12841 atcaaagata tagagcattt ttgcctcctt tcgcgccaca agaaattaac tgtagcggga
    12901 aaggatgggt ttggctatgt ggcaaggtat gaagccaggt taacggtcac aaaaatctac
    12961 gcgtcttcct ggggaagaat gatttcaatt tggctgtctt gtaagcgtga ttttagcgca
    13021 gcgggcggca gctcgtcggt aaagagcgca gtgacctgtg ccacgttacc aatttcaacc
    13081 gccgccgaag catgatactt agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc
    13141 atcatcgttt tcaccacgtt agcttcgttt acatcaaact ccatcaacgc gccatcgctc
    13201 tcaatcgccc caacgcttgt taccagataa tcagcacgaa aatcagccac aaaggacgcc
    13261 gctgaagggc caatgatccc gctattatga gagcgcaacg taccgccggg caccatcact
    13321 tcaaagcgcg ggttgtggta aagaatatgc gccacacgca ggctgttggt gattatccgc
    13381 aaatgattat ggttaagtaa cgcccgggca acatgctcaa cagtcgtacc aatggtgata
    13441 aatattgttg aaccatcagg aatatagtct gccaccgctt cggcaatcgc ttttttttcc
    13501 tcggtttgcg aaacctcacg ctgctcgaac gccgtattaa cgacgctgga agcccgaccc
    13561 gcgccaccgt gatggcgcgt aatcaggcct tgctcgctta atttacgaat atcccggcgg
    13621 accgtctgcg tggagacatc cagcagattt gccagctcat caatattcat atacccacgc
    13681 tcggcgatca gcatgaggag ttgatcgtgc cgcgggttac cggttagttc ggtaaggctc
    13741 atgaaaattc ctcgaaaaac catcaccctg gcattttata caaaaagtga caaaaaagat
    13801 cgggtttgat cacagtcagg gatcccggct cgtccaccgg gacgtgtgca ttttaacgcc
    13861 gctacaccgc tggcgaagcg gactgactcc gctaaatccc cacttgttgc cagcgccacc
    13921 gccaaagcgc cgtgaaaaac atcacccgca cctgtggtat ctaccacatc aactttgaag
    13981 gccggttgat gctgacgccc accattttcc agccagtcgc agcctgcgct accctgggtc
    14041 acatagacat gaccatttgt gagcgtttgt gcctgtttta gcgcactggc catctctttc
    14101 acgcccgtta agcgcgccag acccggttct gaaaaggccg cgtgatcgct taatgccacc
    14161 agctcactga tatcctgcgg cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga
    14221 cgggccaggg tgaaggcttt tttagcgccg tcgtgccagc gtacatctgc cagcacaaca
    14281 tcccactgag agaaatcaat ttcctccaac cactctgcgt caggcagcag atccgggctg
    14341 gggtagttaa tgattatccg ctcgcctttg gtatccacca tgatggcgga ttgcgaagat
    14401 ttcgcctggt tataccgttt ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc
    14461 agcaggctgt tgcccgtgtc gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc
    14521 agccttgccg ccgcaaccgc tgccgtcgcc gctggcccgc caccaacttc cgtataattt
    14581 ctcgccacgt atttaccgct ctccgtcggt aacccttcca cgtaatagat gcgatccatc
    14641 acggttatac ctacacaagc aacacgaatc atggtcattc cttaaacatt ttgggatgcg
    14701 ttcattttaa tttcaccaat tgtttaaaaa gtgacggctg tcaatttttt gacgataaat
    14761 tacaaatacg atcaaaaaca gacaagcaaa gcatgtcata aatgacaaaa agtgacatca
    14821 ctgtattcag gagagggtta tggcagcaat cgcgtttatc ggtttaggac aaatgggttc
    14881 gccaatggcg agcaatttat tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc
    14941 cgaagctgtg cggcatctgg tagacaaagg tgcgactccc gccgccaacc cggcgcaggc
    15001 cgctaaagat gccgaattta tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt
    15061 gttgttcggt gaaaacggcg tttgcgaagg cttatctacc gatgcgctgg tcattgatat
    15121 gtccaccatc catccgctgc aaaccgataa attgattgcc gatatgcaag ccaaaggctt
    15181 cagcatgatg gatgttccgg taggccgtac ttctgcaaat gccattaccg gtactctgtt
    15241 actgctggct ggcggcaccg ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat
    15301 gggcagtgag ttgatcaact ctggcggtcc gggcatgggg atccgcgtta agctcatcaa
    15361 caactacatg agcatcgcgc tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc
    15421 cctgaatctt cccttcgatg ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg
    15481 tcacttcacc acttcctggc caaacaaagt cctcagcgga gatctttctc ccgccttcat
    15541 gatcgatctt gcccataagg atcttggcat cgcccttgat gtcgccaacc agctgcatgt
    15601 gccaatgccg ctgggggccg cctcacggga ggtttatagc caggcgcgcg cagcgggtcg
    15661 cggtcgccag gactggtccg ccattctgga acaggtccgt gtcagtgccg ggatgactgc
    15721 caaagtaaaa atgtaacgac tggataaagg aataaatgaa tgaataagta caccatcaac
    15781 gacattacgc gcgcatcggg cggttttgcc atgctggcgg tcgatcagcg cgaagccatg
    15841 cgcatgatgt ttgccgcggc tggggcaccc gctccggtag ccgatagcgt tttaactgat
    15901 ttcaaagtta acgctgcaaa ggccctctcg ccttatgcct cggcgattct ggtagatcaa
    15961 caattctgct accgccaggt ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt
    16021 gtcgccgccg atgagttcat tcctggcaac ggtattccgg tcgatagcgt ggttattgac
    16081 cgcaaaatca atccgctaca gatcaaacaa gacggtggca aagccttaaa actgctggtg
    16141 ctgtggcgta gtgatgaaga tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa
    16201 ctgtgccact cacacggtct ggtaagcatc attgagccag tcgtccgccc accgcgtcgt
    16261 ggcgataaat tcgatcgcga acaagcgatc atcgatgccg ccaaagagct gggcgacagt
    16321 ggcgctgacc tctacaaagt tgaaatgccg ctttatggca aaggtccgca acaagagctt
    16381 ctctgtgctt cacaacgtct gaatgaccat atcaatatgc catgggtgat cctctcttcc
    16441 ggtgtcgacg aaaaactgtt cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg
    16501 ggattcctgg caggccgtgc agtctgggca tcggtcgtcg gtttaccaga caacgagctg
    16561 atgctgcgtg acgtttgcgc accgaaatta caacaacttg gcgatatcgt cgacgaaatg
    16621 atggctaaac gccgctaaga tgaggataaa aggaatgaaa tggtttaaca ccctaagcca
    16681 caaccgttgg ctggaacagg aaaccgaccg catctttgat tttggtaaaa attcagtagt
    16741 gccgactggt tttggctggt taggcaataa agggcaaatc aaagaagaga tgggcaccca
    16801 tctgtggatc accgctcgta tgttacacgt ttattccgtt gctgcggcga tgggtcgacc
    16861 tggcgcttac tcgttggttg atcacggtat caaagccatg aacggcgcac tgcgtgataa
    16921 aaaatatggc ggctggtatg cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca
    16981 gggctatcaa cacttctttg ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc
    17041 ggaagcgcgc aagctgctcg attacaccat tgaaattatc gagaaatatt tctggagcga
    17101 agaagagcag atgtgcctgg aatcctggga cgaagccttc agcaaaaccg aagagtaccg
    17161 cggcggcaat gccaatatgc acgcggtgga agctttcttg attgtttatg acgtcactca
    17221 cgacaaaaaa tggctggatc gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc
    17281 cagaaataat cattatcgcg ttaacgaaca cttcgatacc cagtggaatc cgctgccgga
    17341 ttacaacaaa gataacccgg cgcatcgctt ccgcgcgttc ggaggtacac caggccactg
    17401 gatcgaatgg ggccgtttaa tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca
    17461 accaccagca tggctgctag aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc
    17521 ctgggcaccc gatggcgcgg acgggattgt ttataccgtt gactgggaag gaaaaccggt
    17581 ggtccgcgaa cgtgtacgtt ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta
    17641 caccgtcact ggcgatcgcc agtatgaaac ctggtatcaa acatggtggg agtactgcat
    17701 taagtacctg atggactatg aaaatggttc ctggtggcag gagctggatg cggacaataa
    17761 ggtcaccacc aaagtctggg acggcaaaca ggatatttat cacctgctgc attgcctggt
    17821 gatcccgcgt atcccgttag cgcccggcat ggctccagcg gttgcagcgg gtctgctgga
    17881 tattaatgcg aaataactga tctgaaaata gggtgcgggt tttcccctca ccctaaccct
    17941 ctccccaaag gggcgagggg accgtccggt gcagtttttg ggtttgacat cagcctcacg
    18001 ggaggctgta tgtcgttaat taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt
    18061 ggacagaccg tttcacttgc cgcgggggat tatcacgcga ccattgtgac cgtcggtgct
    18121 ggtctggctg aattaacctt tcagggttgt catttagtca tcccgcacaa acccgaagag
    18181 atgccgctgg cgcatctggg taaagtgctt attccgtggc cgaaccggat cgccaacggc
    18241 tgttaccgct atcaggggca ggaatatcag ttacccatta atgaacatag ttcaaaagcc
    18301 gctattcacg ggctgcttgc ctggcgagac tggcaaatta gtgaattaac cgccaccagc
    18361 gttaccctga cagcttttct gccgcccagt tacggctatc cgtttatgct ggcttcacaa
    18421 gttgtttatt cgctcaacgc acacacgggt ctttccgttg agatcgcaag ccagaatatc
    18481 ggcactgtcg cagcgcctta tggcgtgggt attcatactt atttaacctg caacttaaca
    18541 tctgttgatg aatatctgtt ccagcttccg gcaaaccagg tttatgccgt tgatgaacac
    18601 gtaaatccga caacgcttca ccacgttgat gagctggatt taaatttcac gcaggcgaaa
    18661 acaatagccg cgacaaaaat cgaccacacc tttaaaaccg ctaatgatct gtgggaaata
    18721 accattaccc atccacaaca ggcgctttcg gttagccttt gttgcgatca accctgggta
    18781 caaatttata gcggtgaaaa attacagcgt cagggactgg cagttgaacc tatgagttgc
    18841 ccgcctaacg cctttaattc aggtatcgac ttattattac tggaaccagg aaaaccgcac
    18901 cgcctgtttt ttaacattta cggacagaga aaataacttg tattgattag cagtcaaaat
    18961 cattgaagaa catttcactc gctaaaggca acattatgta tcttcaattt attaagttaa
    19021 aaacagctca gcttctgtaa agtttttata tatcagtttt atgagaatca ttttacataa
    19081 gcctgtaata acaggaaggc aacaatctcg ttctcaactc tatggagatt agttatggat
    19141 acgccacgtc cacagttatt agattttcaa tttcatcaga ataacgacag ttttacccta
    19201 cattttcaac aacgtcttat tttaacccat agcaaagata atccttgttt atggattggc
    19261 tcaggtatag cggatatcga tatgttccgc ggtaatttca gcattaaaga taaactacag
    19321 gagaaaattg cgcttaccga cgccatcgtc agccagtcac cggatggttg gttaattcat
    19381 ttcagccgtg gttctgacat tagcgccacg ctgaatatct ctgccgacga tcagggccgt
    19441 ttattgctgg aactacaaaa cgacaacctt aaccacaacc gtatctggct gcgccttgcc
    19501 gctcaaccag aggaccatat ctacggctgc ggcgaacagt tttcctactt cgatctgcgc
    19561 ggcaaaccgt tcccgctatg gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat
    19621 gtcacctggc aggccgactg caaagaaaat gcgggcggcg actattactg gactttcttc
    19681 ccacagccta cgtttgtcag cacgcagaag tattactgcc atgttgataa cagttgctat
    19741 atgaacttcg actttagtgc cccggaatac catgaactgg cgctgtggga agacaaagca
    19801 acgctgcgtt ttgaatgtgc tgacacatac atttccctgc tggaaaaatt aaccgccctg
    19861 ctgggacgcc agccagaact gcccgactgg atttatgacg gagtaacgct cggcattcag
    19921 ggcgggacgg aagtgtgcca gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc
    19981 aacggcatct gggcgcagga ctggtccggt attcgtatga cctcttttgg caaacgcgtg
    20041 atgtggaact ggaagtggaa cagcgaaaac tacccgcaac tggattcacg cattaagcag
    20101 tggaatcagg agggcgtgca gttcctggcc tatatcaacc cgtatgttgc cagcgataaa
    20161 gatctctgcg aagaagcggc acaacacggc tatctggcaa aagatgcctc tggcggtgac
    20221 tatctggtgg agtttggcga gttttacggc ggcgttgtcg atctcactaa tccagaagcc
    20281 tacgcctggt tcaaggaagt gatcaaaaag aacatgattg aactcggctg cggcggctgg
    20341 atggctgact tcggcgagta tctgcccacc gacacttacc tgcataacgg catcagcgcc
    20401 gaaattatgc ataacgcctg gcccgcgctg tgggcgaaat gtaactacga agcccttgaa
    20461 gaaacgggca agctcggcga gatccttttc tttatgcgcg ccggttctac cggtagccag
    20521 aaatactcca ccatgatgtg ggcgggcgac cagaacgtcg actggagtct cgacgatggc
    20581 ctggcgtcgg ttgtcccggc ggcgctgtcg ctggcaatga ccggacatgg cctgcaccac
    20641 agcgacattg gcggttacac caccctgttt gagatgaagc gcagcaaaga gctgctgctg
    20701 cgctggtgcg atttcagcgc cttcacgccg atgatgcgca cccacgaagg taaccgtcct
    20761 ggcgacaact ggcagtttga cggcgacgca gaaactatcg cccattttgc ccgtatgacc
    20821 tccgtcttca ccaccctgaa accttacctg aaagaggctg tcgcgctgaa tgcgaagtcc
    20881 ggcctgccgg ttatgcgccc gctgttcctg cattacgaag acgatgcgca cacttacacc
    20941 ctgaaatatc agtacctgtt aggtcgcgac attctggtcg ctccggtgca tgaagaaggc
    21001 cgtagcgact ggacgctcta tctgccggag gataactggg tccacgcctg gacgggtgaa
    21061 gcgttccggg gcggggaagt taccgttaat gcgcccatcg gcaagccgcc ggtcttttat
    21121 cgcgccgata gcgaatgggc ggcactgttc gcgtcgttaa aaagcatcta atcacatccg
    21181 cccgtgggga aactcatggg caaagggaga acatcgatga gtcacatcac aacggaagat
    21241 ccagcaactt tacgcctgcc ctttaaagag aaactctctt acggtattgg cgacctggcc
    21301 tctaacatcc tgctggatat cggtacgctt tatcttttga agttttatac cgacgttctg
    21361 gggctgccag gcacctatgg cggcattatc tttttgattt caaaattctt tactgcgttt
    21421 accgatatgg gtaccggcat tatgttggat tcccgacgca agatcggtcc aaaaggtaag
    21481 ttccgtcctt ttattctgta tgcgtcattc ccggtcacct tactggcgat cgccaacttt
    21541 gtcggcacac cgtttgatgt caccggtaaa acggtgatgg ccactattct gtttatgctt
    21601 tacggactgt ttttcagcat gatgaactgc tcctacggcg caatggttcc cgctatcacc
    21661 aaaaacccca acgaacgcgc ctcactggcg gcatggcgtc agggcggcgc tacgctgggc
    21721 ctgctgctgt gcacggtggg attcgtgcca gtgatgaatc ttatcgaggg taatcagcaa
    21781 cttggctata tcttcgccgc cacgctgttt tcactgttcg gcctgctgtt tatgtggatc
    21841 tgctactcag gcgtgaaaga gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag
    21901 cctggcctgt tgcaatcttt ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt
    21961 attgccaacc tctgcacctt aggggcgttt aacgtcaagc tcgccattca ggtctattac
    22021 acccagtacg tacttaacga tcccatcctg ttgtcgtata tgggattttt cagcatgggc
    22081 tgtattttca tcggcgtgtt cctgatgcct ggcgcagtca gacgttttgg taagaagaag
    22141 gtctatatcg gcggcctgct gatttgggtg ctgggcgatc tgctcaacta tttcttcggc
    22201 ggcggctcgg tcagctttgt ggcgttctcc tgcctggcgt tcttcggctc agcgtttgtt
    22261 aacagcctga actgggcgct ggtttccgac accgtcgagt acggcgagtg gcgcaccggc
    22321 gtgcgttcgg aaggcacggt ctacaccggc ttcaccttct ttcgcaaagt gtctcaggcg
    22381 ctggctggtt tcttccccgg ctggatgctg acgcaaattg gctatgtgcc gaacgtcgca
    22441 caggctgacc acaccattga agggttacgc cagttgatct tcatctaccc aagcgcactg
    22501 gcggtagtca ccattgtggc gatgggttgc ttctacagcc tgaacgagaa gatgtatgtc
    22561 cgcattgtgg aagagataga agcccgtaaa cgcacggcgt aataataata aacgacgccc
    22621 tgcggggcgt tataaggagt gattatgtct gaccataatc cactgacatt aaaactgaat
    22681 ctgcgggaaa aaatcgccta tggtatgggc gacgtcggtt cgaatttaat gctctgcatc
    22741 ggtactctgt atctcctcaa attttatacc gatgaattag ggatgcctgc ttactatggc
    22801 gggattatct ttctggtggc gaagtttttt accgcgttta ccgatatgct caccggattt
    22861 ttactcgact cgcggaaaaa tattgggcct aaaggcaaat ttcgtccctt tattttatat
    22921 gcagcagttc cggcggcgtt aattgcaacg ctccagttta tcgccaccac cttttgtttg
    22981 ccggttaaaa cgacgattgc caccgcgctg tttatgatgt ttggactttc atacagtctg
    23041 atgaactgct cgtatggtgc aatgatcccg gcaattacca aaaacccgaa tgagcgcgcg
    23101 cagctcgcgg cttaccgcca gggtggtgca accatagggc tattgatttg taccgtggcg
    23161 tttattccgt tgcagtcgct tttttctgac tcaaccgtcg gttatgcctg tgcggcactt
    23221 atgttctcca ttggcggctt tatttttatg atgctgtgct acagaggcgt caaagagcat
    23281 tatgtggaca cagcgccaac cggacataaa gccagtattc tcaaatcttt ttgcgcgata
    23341 tttcgtaatc caccattgct ggttttatgc attgctaacc tgtgtactct ggcggcattt
    23401 aatatcaaac tggcgattca ggtctattac acccagtatg tgctgaatga tattaattta
    23461 ttgtcgtgga tgggattctt cagtatggga tgcatcctca tcggcgtatt actggtgcca
    23521 ttaactgtaa aatgttttgg taaaaaacag gtttatctgg ctggcatggt gctgtgggcg
    23581 gtgggtgata tactgaatta tttctgggga agtaactctt tcactttcgt catgttctct
    23641 tgtgtcgcct tttttggcac ggcctttgta aacagcctga actgggcact ggtaccagat
    23701 accgtagatt acggtgaatg gaaaaccggt attcgtgccg aaggttctgt ttataccggt
    23761 tataccttct ttcgtaaaat ttctgccgca cttgctggct tcttgccagg cattatgctg
    23821 acgcaaattg gttatgttcc caacatcgcg caaagcgatg cgacattaca gggtttgcgt
    23881 cagctcattt ttatctggcc ttgtgcactg gcaattattg ctgcattgac gatgggattc
    23941 ttttacacac tcaatgaaaa acggtttgca ttgattattg aggaaatcaa ccaacgcaaa
    24001 aataaagaga tggcgacaga agaaaaaacg gcttccgtaa cgttataaat gtcaatagcc
    24061 gctatttcca tcctggtggg tggcggcctc cctacgttta aaaaatggac ttattatgaa
    24121 aaagattaat gcaataattt tattatcttc tctgacctct gcctcggtat ttgctggcgc
    24181 atacgtcgaa aacagggagg catataatct tgcctccgat cagggcgagg ttatgctacg
    24241 tgtgggttat aacttcgata tgggcgcggg tattatgtta accaatacct acaactttca
    24301 gcgagaagat gaactaaaac atggatataa cgaaattgaa ggctggtatc cgttatttaa
    24361 accaaccgat aaattaacca tccagcccgg tggcttaatt aatgataaga gtatcggttc
    24421 gggtggtgct gtttatctgg atgttaacta caaatttact ccgtggttta acctgacggt
    24481 gcgtaatcgc tataaccata ataattacag ctctacagat ttgaacggcg aattggataa
    24541 taacgacacc tatgaagttg gcacctactg gaactttaaa ataacagaaa aattttccta
    24601 tacatttgaa ccacattact tcatgcgagt gaatgacttt aatagtagca acgggaaaga
    24661 tcatcattgg gaaatcacta acaccttccg ttaccgtatt aatgaacact ggctccctta
    24721 tttcgaattg cgctggttag accgcaacgt cgaaccgtac caccgcgagc aaaaccagat
    24781 ccgtatcggg acgaagtatt tcttctgatg tgaaactgcc ggatgcggcg taaacgcctt
    24841 atccggccta cggttgatac ggttttcgtt ggccggatta agcacatccg gccattaatt
    24901 aagcttcagc cgccagcatc tctttcttac gttggctaat acgcttaacc agcattacgg
    24961 aaaccaccgc accacagaat gcgaagcagg ccataatgcc aaacacgatt ttgtagccaa
    25021 taatccccgg atttaaatcc agaatgtagc catacagact gaagcagaac atcgccgggg
    25081 cgtaaccaat aaagctaccc aacgccatcg ccgcgcctgt tttattttca gcaatttttg
    25141 cttcgccgat aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg ccaaagccca
    25201 gcgtacatgc catccctaaa tagaccggca tactttcgtg cggcagcata atcaacagta
    25261 cgagcgcagc ggtactgatg ataaaggtgt agcatagata tttactcggc gatttcagga
    25321 ttttatctga aatcatgcca ccaatcggtc caccaatcat tttcagacag tattggttaa
    25381 tgatgccgta agcccccacc agcgcaacgg gcaatagata gatgtttttc aggaatggaa
    25441 tgaagaatgt caggccacag taaaccgcgt aaacgaagaa gacgttaaaa gcgataagcc
    25501 agatagtttt gtctttcagc accgaggtca tactggtgtt tttcgatgct ccgtcttctt
    25561 ttttcacctc gacggacggt gcctcttctt tgtcattcag aacaaagaaa atgataatgc
    25621 ctacggcaat cacaataagg gaatagaacc agatgcctgc tttaaaaccc aataagccac
    25681 tgccaaacca ggtaaatact gccaacgcag aaaatgccac cacggtatcg acaatgccac
    25741 gccctgtttc gaagaagcca aacaaccgac cttgttgttc gctattgccc aatcgactta
    25801 ccgacttgag caagaccggc cagttcatca tgtcgcaagt aacgccaaat agcgcccaga
    25861 caaagaggat gccccaatac cccggcattg tcgtcaaata aacacccagt aatcctgtcg
    25921 caatgagtga gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa taaatagaca
    25981 gaaaaaaacc cactgtcgtg acaaatgagt ttaccgacat agcattacca atttgaccat
    26041 tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc gacggtaatt
    26101 tataaattgt accgccacac aatgttaata gactaaataa cgcccatttc tttttcgtga
    26161 gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa gataccgacc
    26221 attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt ggttcagaag
    26281 atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta tctgattaat
    26341 tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg gcaatggtgt
    26401 ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa agcggaacgc
    26461 cggcgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg gctcatcatc
    26521 acccaggcat ataaggtgcg ttaataacgc tttgaacggc atatcaccct gacctgaaat
    26581 acaggcttta tgacccaggc cacccggttc tttaacgatc aaggcatctt tgatatggac
    26641 ctgggtaata tgcggcgcca tcgtttttaa agcgtcgatg ggatgctcat ttgcattgat
    26701 catgttcgca aaatcaaaca gtaaggaaag agattccatc tcactttctt tgaccagcga
    26761 caccagctca tggcttttta aatcttcatg ctgttcgatc gtaaaagtca ggccgctgtc
    26821 ctgatacgtt tcccgtacat aggcaatgtc gttagcgatg atcgataata cgtcgcgcaa
    26881 attaccttca taacgtggat aaaaacgtac ggacgatgcc ccagttttca acgcaatggc
    26941 gacggcttcg tcaatagatg ccttatctga ggcgctggtt tcaatatgaa tatcaaggtt
    27001 cagacggcgg gctttatctc caaaggcaga gagttcttta tcgtccatat taccaagaga
    27061 aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat
    27121 atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa aggcataagc
    27181 atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt
    27241 caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa
    27301 tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgctg
    27361 gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga
    27421 aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact gaagaaaatg
    27481 agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata tatgtttcgt
    27541 gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag agttcagttg
    27601 atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta cggcttttct
    27661 cggtaaaccc ccaggaaggt tcccgccctt gctcaattgc ggcccgctga aagctcgccg
    27721 acagctccgg gctgtaatta aatcgcggct gagtcacgaa ccacccttta cgatccttac
    27781 ggaagatttt ggattctgcc tcgaggttca gcaacgcctg gcgcagcgtc atacgtttaa
    27841 tgcccagcag ctctcccagc tctctttctg aagggagttt tccacccggc gtagtgatgc
    27901 catcttttaa ccaccgatcc agtttctctt ttgcattttc tacggtggat tgatttcccg
    27961 tcattgttta atccttttgg tttaaaccag atgaggcatc ttacgcttac gccaaaaaag
    28021 aaagcaatta acggcgtatg actttgcaga taattgtgac aaagctctct attgacgtaa
    28081 ccacagactt ataagggaga tactcgcatt gctgagtgct cacgacaggc aaaaaaaaac
    28141 ctgccagcga tggcaggttt tttataacta aaaaacgaaa ttaatcgtct ttcggtgcgc
    28201 ggttggcgcg acgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg
    28261 gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc
    28321 ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt
    28381 tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata ccgataatct
    28441 gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga
    28501 acagcgcgaa cgcgaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct
    28561 gacccacttc acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag
    28621 taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca
    28681 cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac
    28741 gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc agagtcacgt
    28801 tttcatatgg ctcttgttta cgaccgtcga tttcacggaa gataactttc ggacgggata
    28861 ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg tgtagttcgc
    28921 cacgaccaga aacgcggaac gcatcagcgt cttcggtttc ttctacgcgc agcgcaacgt
    28981 tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt
    29041 ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa
    29101 cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca
    29161 gttcgccaag gcccgtgatc gccacgatat cgccagcttc cgccagatcg gtttcgatac
    29221 gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt ttgccttcgc
    29281 tatcgatgat agtgacctgt tggttcggct tcactttacc gcgcttgatg cggccaatgc
    29341 cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt
    29401 caaggtcaac gtccggcgca ggaacgtgat caacaatcgc ctgatacagc ggggtcatgt
    29461 cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga
    29521 tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat
    29581 ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct
    29641 tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa
    29701 atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac
    29761 cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag
    29821 cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca
    29881 cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta
    29941 ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga
    30001 tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta
    30061 caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct
    30121 ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caacatggtg cttaatgttt
    30181 ccattgaagc actatattgg tgcaacattc acatcgtggt gcagcccttt tgcacgatgg
    30241 tgcgcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt
    30301 ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac catgaccaat
    30361 ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt
    30421 gaagtttgtt gatttgcgct tcaccgatac taaaggtaaa gaacagcacg tcactatccc
    30481 tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat
    30541 tggcggctgg aaaggcatta acgagtccga catggtgctg atgccagacg catccaccgc
    30601 agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca tccttgaacc
    30661 tggcaccctg caaggctatg accgtgaccc gcgctccatt gcgaagcgcg ccgaagatta
    30721 cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct
    30781 gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta tcgacgatat
    30841 cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc accgtccggc
    30901 agtgaaaggc ggttacttcc cggttccacc ggtagactcg gctcaggata ttcgttctga
    30961 aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc
    31021 gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga
    31081 aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac
    31141 ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtctct
    31201 gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc
    31261 gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc
    31321 gaccaccaac tcttataagc gtctggtccc gggctatgaa gcaccggtaa tgctggctta
    31381 ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg
    31441 tcgtatcgaa gtacgtttcc cggatccggc agctaacccg tacctgtgct ttgctgccct
    31501 gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa
    31561 aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct
    31621 ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt
    31681 cactgacgaa gcaattgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg
    31741 tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta
    31801 ctacccggcg caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
    31861 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
    31921 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
    31981 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaacctg
    32041 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
    32101 tttggtacac cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
    32161 gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt catcgacggg
    32221 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
    32281 atggctccga tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag
    32341 gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
    32401 ttacgtggcg cggcgcagct actcagcaaa gcgttacctg acccatcact actcgaatat
    32461 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttgggg
    32521 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtagctga acgcgtggta
    32581 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
    32641 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
    32701 aatgcgctac aggcgctggg gccggagggt ggtgaaatca ttctgcgtac ccgcaccgcg
    32761 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
    32821 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
    32881 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
    32941 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
    33001 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
    33061 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
    33121 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
    33181 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
    33241 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
    33301 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gccgtcgcgc
    33361 tggttgagcg cgccatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
    33421 acggcccaac gaccgatatc atcggcgaag cgccagccat gcaggacgtg ttccgtatta
    33481 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
    33541 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg cgccaaagcg ccgtttatcg
    33601 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
    33661 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
    33721 gtacattatt cctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
    33781 gcgtgctggc agacggtcag ttttaccgcg ttggcggcta tgcgccggtg aaagtggatg
    33841 tgcggattat cgctgccact caccagaatc tcgaacagcg agtgcaggaa ggtaagttcc
    33901 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
    33961 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
    34021 gcgtagaagc gaagttactg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
    34081 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
    34141 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
    34201 cttcgcaaat gcaaccggac agctgggcga cgcttcttgc gcagtgggca gacagagcgc
    34261 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
    34321 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
    34381 ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga ttcacagctt
    34441 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttacgcttta ctgttccgat
    34501 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
    34561 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
    34621 tgtgtgccgc aatgattggg ctgtttagtt gagtttcaga ttgctgatga agtgctcttt
    34681 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaagcaata ttttcaacag
    34741 gttttgtagg cctgataagc gtagcgcatc aggcaatctt gttctcagca cattgatggc
    34801 gctttcgttt tctactttgt aaacgaagcg ccattcacta tttaaatcac ccgtgagaac
    34861 tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat gttgcggatc
    34921 agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac cagcccatct
    34981 tttgctaacg gggcgagcag cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc
    35041 cactgtttct caataggggc gtaatccaga cggaagttgc agatgagcga cttaatcaca
    35101 tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc attgccttgt
    35161 tcatccactt gctgatagta ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca
    35221 atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg agtggtgtag
    35281 ccctggaagt tacgatgcag cacgccttca cgctgggcca ccgccagctc gtcatccgga
    35341 cgggcaaagt gatccatacc gataaactga tagcccgatt gcgtcaggaa ggcgatggtt
    35401 tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc
    35461 tgagcagcaa aaatggtcgg cagatgcgcg tagttaaaga cactcagacg atcggggttc
    35521 agctccgcca cacgtttcag ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg
    35581 taaatcaggt cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca
    35641 aagatgaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt gttgaagtcc
    35701 tgcacgccca tgctcaggcg attaaagcct tcggcgcgta aatgatcgag tacatccagt
    35761 tcgatttccc gcggatcgac ttcgatcgaa atctccgcat cggcattgaa ctggaagttt
    35821 tcgcgcagca gcttcatcag gcggctgatt tgcgctttat tcagatacgt cggcgttccg
    35881 ccgccccagt gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt
    35941 tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt aacaatctta
    36001 ttgcaaccgc agaagtaaca aagcttatgg cagaacggga tatgtacgta gagagataat
    36061 ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa
    36121 aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata tttctggatc
    36181 agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga tggcgtctct
    36241 ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg cgcagccact
    36301 tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa catataaccg
    36361 gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg ccccgcagca
    36421 gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga
    36481 ggccgagttt ctgcatcaac tcatcaatac gatccagttt agcatccacc caggattgct
    36541 cttcggcact cagggtttcg cctgcttcca gacgttccag cagcgcatcc agacgctcat
    36601 ccgtttccag taattccaac tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt
    36661 tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat aggggtttta ctgccaatac
    36721 gtggatcttt tggtgcgttc tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc
    36781 ggctgcccgg cgcatgacca cgacgttttt tctgacgctt gcgatcacga gcttcctgat
    36841 ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag
    36901 atgatggttt cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga
    36961 atctaacaga aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg
    37021 actcataacc cttaacgggt cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc
    37081 tttagccgga acaatccgtg ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt
    37141 cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg accctcatcc tgagtcattg
    37201 ttcctgttag cgtcctcgct ctatgggtgc tactttacgc ctttgcttta aacaaacaat
    37261 caaccgccat cagtatttgc acattttcag agttttatat aaaagcaatt cactgattta
    37321 tatagcttat ttgacatgca tcgcagaatt ttctctgcta tttcgcataa agactatcgg
    37381 caatgtctca caaagcgtat ggcttttact tacaacgctc acgccgaaaa atccgccttt
    37441 tattatggtt caggtataat cgccacaaag ctttgactga cggagacgac cgctttgact
    37501 aatttgaatt atcaacagac gcattttgtg atgagtgcgc ctgatattcg ccacctacct
    37561 tccgataccg gaattgaagt ggcttttgca ggccgttcca acgcaggtaa atccagcgcg
    37621 ctgaacacgc tgactaacca gaaaagcctg gctcgtacct caaaaacccc agggcgcacc
    37681 cagcttatca acctgtttga agtggctgac ggcaagcgtc tggttgactt gcctgggtac
    37741 ggttatgcgg aagtcccgga agagatgaag cgcaaatggc agcgtgcgct cggcgaatac
    37801 ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa tggatattcg ccatccgctg
    37861 aaagatttgg atcagcagat gattgagtgg gcggtagaca gcaatatcgc cgttctggtg
    37921 ctgctgacca aagcggacaa actggcaagc ggcgcacgta aagcgcaatt gaatatggtg
    37981 cgtgaagctg tactggcgtt taacggtgat gtgcaggttg aaacgttttc ttcgttgaag
    38041 aaacaaggcg tggacaagct gcggcagaaa ctggatacct ggtttagcga gatgcagcct
    38101 gtagaagaaa cgcaggacgg cgaataattt tcttgcctta atgcttgtgc cggatgtggc
    38161 gtatccggcc cgtaaattca atagattaca attctaccgt tggcctgata aacatagcgc
    38221 atcaggcatt acggatcttt tctttcgccc aataaaaaac gccccagtca ttactgactg
    38281 gggcggctaa aatattcagc caaatccgat tacgtgaagt aaaaggtctg aaagatagaa
    38341 catcttacct ctgtacccta cgcgactaac tttactcttt tttgttcagt tcagaaagca
    38401 ctttttgtaa ttttttttca gttgttgcat aggaatttct aatagccatc acaaaacgtg
    38461 acagcttatg ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg
    38521 atcccagttt tcgccactcc ccacttccac cagcaacggc acatccagac gggtacagtt
    38581 ttccatcagt tgatgaatct gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa
    38641 taccagttca tcgtgtacct gcatgatcat acgtacacgc ggttgctcag cctgtaacca
    38701 cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg gcggttccct gcattggcgc
    38761 gttaatggct gcacgttcag ccgctgcacg acgagcacca ttgctggatt tgatatccgg
    38821 cagatacaga cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg cctgagcacg
    38881 ggtgcgttcc atatactcca gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt
    38941 ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac tcatgccata
    39001 aatcagacca aagttgatcg ctttcgcgct acggcgttgc tcgctggtga cggtttccag
    39061 tggcaaacca aacacttctg ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc
    39121 ggtcagcaag cctttgtcac gcgaaagatg cgccataatg cgcagttcaa tctgcgagta
    39181 gtccgctgag acaatcacat aatcctctgg cgcaataaac gcctggcgga tacgacgacc
    39241 ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg gttgacgata aacgtcccgt
    39301 tgcagttact gcctggtgat aagaggtatg cacacgcccg gttttcgggt tgatcatcag
    39361 cggcagcttg tcggtgtagg tcgatttcag cttcgccaga ccacgatact ccagaatcac
    39421 ttttggcaac ggatagtcca gcgccagttc ttccagtacc tcttccgacg ttgacggcgc
    39481 gccacccggc gttttcttca gcggtttaat accctgtttt tcaaagagaa tggtttgtaa
    39541 ctgcttggtg gaagaaaggt taaattcctc acctgcaatt tcatgcgctt tcttttccag
    39601 ctcagccaga cgaagggtga gctcttcaga atgattgtgc agcactttcg gatcgatctt
    39661 cacaccgtta cgttcaatgc gtgaaagcac tggcaccagc ggcatttcga tattctcgaa
    39721 gacgttcaac ggccctttgt gtttttgcag atccggccac attttcagat gcaactgcaa
    39781 ggtgacatct gcatcttcgg cggcgtaacg tccggcttct tcgagggcaa tctggttaaa
    39841 ggtcagttga tttttgcctt taccagcaat ctcttcaaaa gtgatggttt tgtgcttcaa
    39901 ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca acgctattga gaatgtagga
    39961 ctccagcatg gtatcaaacg caatcccacg cagttcaatg ccgtagttcg ccagaatacc
    40021 gcgatcgtat ttcaggtttt gcccgacctt cagcgccttt tcatcttcca gcagcggttt
    40081 tagcaactcg agtgcacgct cgcgagagat ttgatcgggc gcatcaagat aatcatgagc
    40141 aaccggaata tatgccgcta cgcctggctc gatagcaaaa gaaagcccga ccaggttagc
    40201 agagatgtta tcaaggctgt cggtttcggt atcaaatgca aataccggcg ctttttccag
    40261 cttcgcaatc cacgctttca gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga
    40321 aatcaccgtt gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct gtggcttcgc
    40381 ggctggtttt gcccctttgg cctgtaacca tttgcccgct tcgacatcag cagtccagcg
    40441 tttgaactca tactttttga acagccccaa caactcttcc gctgccggtt gctgcacttc
    40501 cagttgttca caggtcagct ccagttcaac gtcggtttta atcgtcgcca gctggtatga
    40561 gagataagca acttctttgt tttgctcgag cttcgctgcc attgttttcg cgccacggaa
    40621 gctcaaccca gcaatttttt ctggctcggc atacagcgta tccagtccgc caagaccttg
    40681 cagcaatgcc tgcgcggttt tttcaccgac gcccggtacg ccaggaatgt tatcagagga
    40741 gtcacccatc agcgccagga aatcgatgat cagttctggc ggcacgccgt acttattcac
    40801 cacctcttcc ggtccgagga tggtattcgt catggtattg ataagcgtaa tatttggcgt
    40861 caccagctgc gccatatctt tatcgccagt gctgatcagc accggacgcc cggctttttc
    40921 ggcttcgcgc gccagagtac cgataacgtc gtccgcttct acgccagaaa ccgccagcag
    40981 cggcagtccc atcgctttaa ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc
    41041 cggcattggc gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc
    41101 cttggcgtca aagaccaccg ctgcatgcgt cggtttatat tgcatgatca gactgcgcag
    41161 catgttgagg acaccataca tcgcaccggt cggctcgcct gcgctgttag tcagcggggg
    41221 aaacgcgtga tatgcgcgat aaagataaga tgaaccatct acaaggataa gtggattttg
    41281 ggggatctga accataatgt ccgtgcctgt ttatcagatt atgggtaaag gatgccacag
    41341 acaggataaa aacatgagtt tttcaccgct tttcgtgaaa atcttttgta gatcttctgg
    41401 atcgctcgca aatcgtcatg tggataactt tgtgcataaa ttatttacat
//
LOCUS       DAMZII010000028        40328 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZII010000028 DAMZII010000000
VERSION     DAMZII010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.028"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            140..718
                     /locus_tag="POA77_001453"
     CDS             140..718
                     /locus_tag="POA77_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDI6364914.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITLKTSEFVLEADHTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            715..1074
                     /locus_tag="POA77_001454"
     CDS             715..1074
                     /locus_tag="POA77_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDI6364915.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            1061..1969
                     /locus_tag="POA77_001455"
     CDS             1061..1969
                     /locus_tag="POA77_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDI6364916.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLTERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAHAVMVAYAMGGDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVENALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTEWSVTNGGTDE"
     gene            1962..2567
                     /locus_tag="POA77_001456"
     CDS             1962..2567
                     /locus_tag="POA77_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDI6364917.1"
                     /translation="MNSLLPSGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            2564..4294
                     /locus_tag="POA77_001457"
     CDS             2564..4294
                     /locus_tag="POA77_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6364918.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGTLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRHYVDQQIEIHE
                     QSRRHPSASLTEKGFVRLYSGVESNDETVAATPKAVKIAMDNASARLAKDRNGSDIPN
                     PALFVQNLGLKTTVDKAASAIQPGDYGIGLAYLKTMGTKSQFFAYGTAVGYPEVPTHG
                     AGFQACYNDNRRAQIYVANDGKMYCRFSMLSKIADDETPWNQVFTSAHYPEASVSVKG
                     MVQLGNDVNSVSENVAATLKAVKIAMDNASARLAKDRNGADIPDPTLFIQNLGLKPTV
                     DKAESALQQGAYGIGSNNNYEMGEVSQFLAYSGNADEVPSNGAGFQAAYNKNRRAQIF
                     ITGAGEMYHRFSGSDTVKDNTTLWRRGVCEDEFSFGSNYHRITGGVLKQFFNSYFSGA
                     TGVVNKEYQVNFPTPFARQCWYVIPVFRSSHGGSVEGVAITAITATGFTLSITGDNGG
                     WNIGFIAEGV"
     gene            4294..4728
                     /locus_tag="POA77_001458"
     CDS             4294..4728
                     /locus_tag="POA77_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI6364919.1"
                     /translation="MSYFYSPSRNAFYNSELKSDYYDALDAWPDDCIEVSDAVYQEFY
                     LGYREGYKMVAGTDNQPSWEERPPLTHEEQVEQAEMMKQMRINEANNLINEKQWPSKL
                     QLGRLNEMETTRFNAMLDYLELLENINVSDAPDIVWPLSPEV"
     gene            4861..6033
                     /locus_tag="POA77_001459"
     CDS             4861..6033
                     /locus_tag="POA77_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDI6364920.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLTDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            6043..6558
                     /locus_tag="POA77_001460"
     CDS             6043..6558
                     /locus_tag="POA77_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDI6364921.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKSTVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            6613..6915
                     /locus_tag="POA77_001461"
     CDS             6613..6915
                     /locus_tag="POA77_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDI6364922.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            6930..7049
                     /locus_tag="POA77_001462"
     CDS             6930..7049
                     /locus_tag="POA77_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDI6364923.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            7042..10119
                     /locus_tag="POA77_001463"
     CDS             7042..10119
                     /locus_tag="POA77_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6364924.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATIQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGASRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLSSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAQVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGTVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMTPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAMVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            10116..10601
                     /locus_tag="POA77_001464"
     CDS             10116..10601
                     /locus_tag="POA77_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6364925.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGMIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            10598..11698
                     /locus_tag="POA77_001465"
     CDS             10598..11698
                     /locus_tag="POA77_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDI6364926.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVISITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            11789..12007
                     /locus_tag="POA77_001466"
     CDS             11789..12007
                     /locus_tag="POA77_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI6364927.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(12243..13928)
                     /locus_tag="POA77_001467"
     CDS             complement(12243..13928)
                     /locus_tag="POA77_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDI6364928.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            14198..14575
                     /locus_tag="POA77_001468"
     CDS             14198..14575
                     /locus_tag="POA77_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDI6364929.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(14605..14862)
                     /gene="grxA"
                     /locus_tag="POA77_001469"
     CDS             complement(14605..14862)
                     /gene="grxA"
                     /locus_tag="POA77_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDI6364930.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            15022..15309
                     /locus_tag="POA77_001470"
     CDS             15022..15309
                     /locus_tag="POA77_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDI6364931.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            15293..16015
                     /gene="nfsA"
                     /locus_tag="POA77_001471"
     CDS             15293..16015
                     /gene="nfsA"
                     /locus_tag="POA77_001471"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDI6364932.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            16076..16978
                     /gene="rimK"
                     /locus_tag="POA77_001472"
     CDS             16076..16978
                     /gene="rimK"
                     /locus_tag="POA77_001472"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDI6364933.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            17066..17542
                     /locus_tag="POA77_001473"
     CDS             17066..17542
                     /locus_tag="POA77_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDI6364934.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            17893..19005
                     /gene="potF"
                     /locus_tag="POA77_001474"
     CDS             17893..19005
                     /gene="potF"
                     /locus_tag="POA77_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDI6364935.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            19100..20233
                     /gene="potG"
                     /locus_tag="POA77_001475"
     CDS             19100..20233
                     /gene="potG"
                     /locus_tag="POA77_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDI6364936.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIVSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            20243..21196
                     /gene="potH"
                     /locus_tag="POA77_001476"
     CDS             20243..21196
                     /gene="potH"
                     /locus_tag="POA77_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDI6364937.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            21193..22038
                     /gene="potI"
                     /locus_tag="POA77_001477"
     CDS             21193..22038
                     /gene="potI"
                     /locus_tag="POA77_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDI6364938.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            22098..22586
                     /locus_tag="POA77_001478"
     CDS             22098..22586
                     /locus_tag="POA77_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDI6364939.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            22627..23754
                     /gene="rlmC"
                     /locus_tag="POA77_001479"
     CDS             22627..23754
                     /gene="rlmC"
                     /locus_tag="POA77_001479"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDI6364940.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(23929..24660)
                     /gene="artJ"
                     /locus_tag="POA77_001480"
     CDS             complement(23929..24660)
                     /gene="artJ"
                     /locus_tag="POA77_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDI6364941.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(24952..25620)
                     /gene="artM"
                     /locus_tag="POA77_001481"
     CDS             complement(24952..25620)
                     /gene="artM"
                     /locus_tag="POA77_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDI6364942.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(25620..26336)
                     /gene="artQ"
                     /locus_tag="POA77_001482"
     CDS             complement(25620..26336)
                     /gene="artQ"
                     /locus_tag="POA77_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDI6364943.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(26343..27074)
                     /gene="artJ"
                     /locus_tag="POA77_001483"
     CDS             complement(26343..27074)
                     /gene="artJ"
                     /locus_tag="POA77_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6364944.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(27092..27820)
                     /gene="artP"
                     /locus_tag="POA77_001484"
     CDS             complement(27092..27820)
                     /gene="artP"
                     /locus_tag="POA77_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDI6364945.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(28038..28553)
                     /locus_tag="POA77_001485"
     CDS             complement(28038..28553)
                     /locus_tag="POA77_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6364946.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            28679..29002
                     /locus_tag="POA77_001486"
     CDS             28679..29002
                     /locus_tag="POA77_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDI6364947.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            28999..29829
                     /gene="amiD"
                     /locus_tag="POA77_001487"
     CDS             28999..29829
                     /gene="amiD"
                     /locus_tag="POA77_001487"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDI6364948.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(29826..30839)
                     /locus_tag="POA77_001488"
     CDS             complement(29826..30839)
                     /locus_tag="POA77_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6364949.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(30938..32368)
                     /locus_tag="POA77_001489"
     CDS             complement(30938..32368)
                     /locus_tag="POA77_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDI6364950.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(32379..33380)
                     /gene="ltaE"
                     /locus_tag="POA77_001490"
     CDS             complement(32379..33380)
                     /gene="ltaE"
                     /locus_tag="POA77_001490"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDI6364951.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(33417..35135)
                     /gene="poxB"
                     /locus_tag="POA77_001491"
     CDS             complement(33417..35135)
                     /gene="poxB"
                     /locus_tag="POA77_001491"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDI6364952.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHAEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(35268..36236)
                     /gene="hcr"
                     /locus_tag="POA77_001492"
     CDS             complement(35268..36236)
                     /gene="hcr"
                     /locus_tag="POA77_001492"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDI6364953.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(36248..37900)
                     /gene="hcp"
                     /locus_tag="POA77_001493"
                     /gene_synonym="priS"
     CDS             complement(36248..37900)
                     /gene="hcp"
                     /locus_tag="POA77_001493"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDI6364954.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(38044..38943)
                     /gene="lysO"
                     /locus_tag="POA77_001494"
     CDS             complement(38044..38943)
                     /gene="lysO"
                     /locus_tag="POA77_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDI6364955.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(39438..40133)
                     /gene="aqpZ"
                     /locus_tag="POA77_001495"
     CDS             complement(39438..40133)
                     /gene="aqpZ"
                     /locus_tag="POA77_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDI6364956.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9768 a        10203 c        11021 g         9336 t
ORIGIN      
        1 tttattaaac caaatcgcct catcatgaga actgggacag tttactatct actgaattgt
       61 ttctaaagag aattgtatca agttccagac aattacaatc cattttggac acgcccaata
      121 tgtggcaatt ttttaagcta tgaacgcaca attaaccgaa atcatgcgcc ttatcaccaa
      181 cctgatccgc actggggtag tcaccgaagt ggaccgggag aactggcttt gccgggtgaa
      241 aacgggcgac cttgaaacca actggatcag ctggctgacg ctgcgtgccg ggaatgcccg
      301 cacatggtgg cgaccatcgg aaggtgaaca ggtggtgctg ctgagtctgg gcggaaatct
      361 tgaaaccgcc tttgcgctgc ccgccatcta ttcgaatcag ttcgcaccac cgtcgacgtc
      421 ggcggacgcc tgcgtgacag aacatcctga cggtggctgg tttgaatacg aacccgccac
      481 cgggcgctgg tatgtcagag gcatcaaatc aatggtcatt gaggccgctg acaacatcac
      541 cctgaaaacc agtgagtttg tgctggaggc tgaccacacg cgtattaaca gcgaagtggt
      601 gatcaatggt ggcgttaccc agggcggcgg tgcaatgagt tctaacggaa ttgtggttga
      661 tgcacatcag catactggcg tcctgaaagg cggcgacaca accggaggcc cggtatgacg
      721 ctttatagcg ggatgaacaa taccagcggc aaagctatta ctgatattga ccatctgcgc
      781 cagtcggtgc gggacattct gctgacaccg cagggtagcc gcattgcccg ccgggaatat
      841 ggttccctgc tgtcggcact gatagaccag ccacaaaatc cggcgttacg cctgcaggtc
      901 atgtcggcag tgtatgtggc gctgagtcgc tgggagccac ggctgacgct ggattccatc
      961 accatcaaca gcaactttga cggttctatg gtggtggagc tgaccgggcg gcggaataac
     1021 ggtgtgcctg tgtccctttc cgtatcaaca ggagcagaga atggcagtga ttgacctttc
     1081 gcagttgcct gcgccgcaga ttgtggatgt gccggacttt gagacgctgc ttaccgaacg
     1141 caaggcagaa tttgtggcgc ttcatccgaa agatgagcag gaagcagtga tccgcacgct
     1201 ggaactggaa tctgaacccg tcaccaaatt gttgcaggag aatgcttacc gtgagttgct
     1261 tctgcgccag cgcattaacg aagccgcgca tgctgtgatg gtggcttacg cgatgggcgg
     1321 cgatcttgac cagctcgctg ccaactacaa cgtgaaacgc ctgacggtga cgcctgctga
     1381 taatgacgct gtgccacccg ttgcggctgt gatggaaagc gatgaagcgt tacgcctgcg
     1441 tgtgcctgcg gcttttgaag ggctttcggt tgcggggcca accgcagctt atgaatttca
     1501 tgcccgaagc gccgacggtc gggtggcgga tgccagtgca accagcccgg cacctgcaga
     1561 ggtggtactg actgtcctta gccgcgaagg cgacggaaca gcagaaaaag acctgctgga
     1621 tgtggtggag aacgccctga acagtgagaa cgtccgcccg gtggctgacc gtctgacggt
     1681 tcgcagcgca gaaatcatac cgtaccgcgt ggaagccacc atttttctct atccggggcc
     1741 ggaagcagag ccggtaatgg cagcggcaaa agccagcctg cagaagtaca tcgccagtca
     1801 gacgcggctt ggtcgggata ttcgccgtag cgccatcttt gccgccctgc atgttgaggg
     1861 tgtgcagcgt gtggagctgg cttcgccgct ggcggatgtg gttttgaaca aaacgcaggc
     1921 ggcatcatgt acggagtgga gcgtgaccaa cgggggaacg gatgaatagt ctgctgccat
     1981 cggggtcaac ttcactggag cgccgactgg cgcagacctg tagcgggatt tctgatctgc
     2041 aggtgccgct gcgtgacttg tggaatccgg ctacctgccc ggtcagcttc ctgccttatc
     2101 tcgcctgggc gttctctgtg gatcgctggg acgaaggctg gacagaaagc gtcaagcgcc
     2161 aggtggtgaa ggatgctttt tatattcatc agcataaagg gaccaccagt gccgtgcggc
     2221 gggtggtgga gccgttcggc tttctgatcc gcattattga gtggtggcag accggagagg
     2281 caccgggcac gtttcgtctg gatatcggcg tgcaggacca gggcatcact gaagatacct
     2341 atctggaact tgagcggctg ataagcgatg ccaaaccatg tagccgtcac atgatcggca
     2401 tgtccatcaa tctgcagacc agcggtccgc attgggtggg ggccgccagc tatcttggcg
     2461 aagaaatcac gatctatccg tatatcaacg aaacaattat ttccggcggc accgcgcatg
     2521 aaggtggggc ggtccatgtt attgacacaa tgagagtgaa tccatgagca caaaatttta
     2581 taccctgctg acggatattg gcgcggcgaa acttgccagc gccgccgcgc tcggtgtgcc
     2641 tttaaaaatt acccatatgg cggtcggcga tggcggcgga acattgccaa cgccggacgc
     2701 aaagcagaca gcattggtaa atgagaaacg ccgggctgcg ctgaatatgc tctatatcga
     2761 cccgcagaac agcagccaga ttattgccga acaggtgatc ccagaaaacg agggcggttg
     2821 gtggatacgt gaagtgggcc tgtttgatga gtccggggca ttgattgccg tgggcaactg
     2881 cccggaaagc tataagccac aactggctga aggcagcggg cgcacccaga ccgtgcgcat
     2941 ggtgctgatt accagcagta cggacaatat caccctgaaa atcgaccctg ctgtcgtgct
     3001 ggcaacccgt cattatgtag atcaacaaat agaaattcat gagcaatcgc gccgccatcc
     3061 ttctgcgtct ttgacagaaa agggatttgt acgtctgtat agcggtgtgg aaagcaatga
     3121 tgaaacagtc gctgcaacgc caaaagcagt gaaaatagcg atggataatg ccagcgccag
     3181 actggcaaaa gaccgcaatg gttctgatat tcccaatccg gcactgtttg ttcagaatct
     3241 gggattgaaa acgactgttg ataaagctgc atcagcgatt cagccaggag attatggaat
     3301 tggcctggct tatctgaaaa ccatgggaac caaatcgcaa ttttttgctt atggtacagc
     3361 tgtcggctat ccagaagtgc caacacatgg tgctggtttc caggcctgtt acaacgacaa
     3421 tcgacgtgca cagatttatg tcgctaatga tggcaagatg tactgtcgat tcagcatgtt
     3481 atcgaaaatt gccgatgatg aaacaccgtg gaatcaggtg ttcaccagtg cacattatcc
     3541 agaagcatca gtgagtgtta aaggaatggt gcaactgggg aacgatgtaa acagcgtgtc
     3601 agaaaatgtt gcggcgacat tgaaagcggt aaaaattgcg atggacaacg ccagcgcgag
     3661 actggcaaag gaccgcaatg gtgctgatat tcctgacccg actttgttta ttcagaatct
     3721 gggacttaaa ccgacggttg ataaggctga aagtgctctg caacagggag catacggaat
     3781 cggtagcaat aataactacg aaatgggtga ggtttcacag tttcttgcat attcaggtaa
     3841 tgcagatgaa gtgccatcga atggtgcagg ttttcaggct gcgtataaca agaacagacg
     3901 cgcgcagatt ttcatcaccg gagcagggga aatgtatcac cgtttcagcg gctctgatac
     3961 ggttaaagac aatacaacac tatggcgtcg gggtgtttgt gaagatgaat tttcatttgg
     4021 cagcaattat caccgaataa ctggcggtgt gctgaagcag ttttttaaca gttatttttc
     4081 cggggcaact ggtgtggtga ataaagagta tcaggtgaat tttccgacac catttgcccg
     4141 tcagtgctgg tacgtgatcc ccgttttccg ttcgtcacac ggtgggagtg ttgagggtgt
     4201 cgccattaca gctattactg caactggctt tactctgagc atcaccggag ataacggtgg
     4261 atggaatatt gggtttattg cggagggggt ctgatgagtt atttttacag tccatcaaga
     4321 aatgcatttt ataacagtga actgaaatct gattattacg atgctctcga tgcgtggccg
     4381 gatgactgta ttgaggtaag tgatgcagtc tatcaggagt tttatcttgg ttatcgggaa
     4441 gggtacaaaa tggttgcagg cactgataac cagccatcat gggaagaacg tccaccattg
     4501 acccatgagg aacaggtgga acaggcagaa atgatgaaac agatgcgcat taatgaggct
     4561 aacaacctga ttaatgaaaa acaatggcca tcaaaattac agcttggcag gctgaacgaa
     4621 atggagacaa ccagatttaa tgcgatgctg gattatcttg agttacttga aaatattaat
     4681 gtaagtgatg ccccggatat agtctggcct ttatcaccag aagtctgaat acatccccgc
     4741 gtctgcgggg attcctgtat ccgccgttgt gccatttttc atacaaacca cataacgtgc
     4801 atcctgctcg tatcaaccag aacataggca gaccccctct actaccggag agactgcctt
     4861 atggctcagg attaccacca cggggtgcgc gttgttgaag tcaacgaagg cacccgatcc
     4921 attaccacgg tgagcaccgc catcgtgggt atggtctgca cgggcgatga tgccgatgca
     4981 aaaatgtttc ctcttaataa acccgtgctg atcactgatg tgctgactgc cagcggtaaa
     5041 gcgggtgagt ccggtacgct ggcccgttcg ctggacgcca tcgctgacca ggcaaaaccc
     5101 gtgaccgttg ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac gaccaatatc
     5161 atcggcgcag tgactgctga aggtaaaaaa acaggcatga aagccctgtt atctgcccag
     5221 tcacagctcg gcgttaaacc gcgcattctc ggcgtgccag gtcacgacac gaaggcggta
     5281 gctactgagt tgctgagcgt ggcgcaaagc ctgcgtgggt ttgcttacct gtcagcgtat
     5341 ggttgcaaga cggtgcagga agcaatcact taccgtgaaa acttcagtca gcgtgaagga
     5401 atgctgatct ggcctgactt tactggctgg gacacggtgc tgaatgccga agcaacggca
     5461 tatgccaccg cccgtgcgct tggtctgcgc gccaaaattg acgagcagac cggatggcac
     5521 aaaagcctgt ccaacgtggg cgtgaacggt gtcaccggaa tttctgcaga tgtgttctgg
     5581 gatctgcagg acccggcaac tgatgcggga ctgcttaacc agaacgacgt caccacgctt
     5641 gtgcgcaagg atggtttccg cttctggggt tcccgctgtc tgagcgatga tccgcttttt
     5701 gctttcgaaa actacactcg cacggcgcag gtgctgacgg acacaatggc agaagcgcac
     5761 atgtgggcgg tggacaaacc gctgaacccg tcgctggcgc gcgacattat cgagggtatc
     5821 cgcgccaaaa tgcgcagcct ggtaagtcag gggtatctca ttggtggtga ttgctggctg
     5881 gacgagtcgg tgaacgacaa agacaccctg aaagccggaa aactcaccat cgattacgac
     5941 tacacgccag tgccgccact tgaaaacctg atgctgcgtc agcgcatcac cgatcagtac
     6001 ctggtgaatt tctccagcca ggtcagcgcg taaggggaca acatggcttt accacgcaaa
     6061 ttaaaacacc tgaacctgtt taatgacggg aacaactggc agggaatcgt tgagtcgctg
     6121 acgctgccga aattcacccg caaatatgag aagtatcgcg gcggcggaat gccgggggcg
     6181 gtggatgtgg atctgggact ggatgacagt gcactggata cagaattttc cattggtggt
     6241 actgaactgc tgctgtttaa gcagatgggc aaatccacgg tggatggcat ccagctgcgc
     6301 tttaccggct ctatccagcg tgacgatacc ggggaagtgc aggccgtgga gcttgtcgtg
     6361 cgtggacgtc acaaagaagt ggattccggc gagtggaaga cgggcgaaag caacaccacc
     6421 aaagtgacca gtaccaacag ctacgcgaag ctgaccatca atggtgaggt gctctatgaa
     6481 gtggacctta tcaacatggt ggaaattgtg gacggtgtgg acctgatgga agcgcaccgc
     6541 aacgccctcg gcctctgata tatctgaacg gcgcgggata ccgcgccaga acccaattga
     6601 caggacagca aaatgagcga taagcagact gaaaagacca ttcaactgga tacccccatc
     6661 aagcgcggta aaacagaaat caccgaaatt gtgctgcgta aaccgcagtc cggtgcgctg
     6721 cgcggtacac gcctgcaggc cattatggat atggatgtaa acgcgatgat gaccgtgatc
     6781 ccccgcatct ccagtccggc actgactgca caggaaattg cagagatgga cccggcagat
     6841 ctcactgcca tgtcggttga ggttgtcact tttttgttga agaagtcggt gcttgccggt
     6901 ttaccgacag cctgacggtt gacgatctgg tggcagatat cgccaccatt tttcactggc
     6961 cgccatccgt tactgacgtt atgccgctga ccgaagtgct ggaatggcgg tataaagcga
     7021 ttcagagaag cggggccaac gatgagtgat aacaacctgc gtctgcaggt cattcttaat
     7081 gcggttgaca agctcacccg cccatttcga tctgcgcagg ccagttcaag agaactggct
     7141 gctgctgtca aaaaatcccg cgatgcaata aagcagcttg atcaggccgg gagcagtctg
     7201 gacagcttcc gaaagctgca ggcagaaaat cagaaattag gcgacaggct gaactatgcc
     7261 cgccagcgtg caaatttgct cagtcaggaa ctgggagcga tggggccgcc ttcgcaacgt
     7321 caggttgttg ctctgggccg tcaacggctg gctgttcagc gcctggaaga acgccagaaa
     7381 aagctgcagc agcagacggc gcttgtgcgt gctgaactgt accgggcggg aatttctgcg
     7441 aaagatgatg cgggagcaac tgcccgttta gcccgtgaaa catcacgtta taaccaggaa
     7501 ctgtcgaaac aggaggcgcg gctgaagcga ctgggggaag ctcagcgcag gatgaatgca
     7561 gcgcgtgcca gttatgcccg ttcgctggag gtgcgtgatc gtattgcagg tgccggagcc
     7621 actaccacgg cggcagggct ggcaatgggc gcgccagtga tggcggcagt aaaaagctat
     7681 accagcatgg aagatgccat gaaaggtgtg gcaaagcagg tcaatggtct gcgtgacgat
     7741 aatggcaacc gcactgcacg tttttatgaa atgcaggatg ccatcaaggc tgccagcgaa
     7801 cagttgccga tggaaaacgg tgcggtagac ttcgctgcac tggttgaagg tggtgcgcgc
     7861 atgaacgtcg caaaccctga cgacagctgg gaagaccaga aacgtgacct gctggccttc
     7921 gccagcacgg cagcaaaggc ggcaacagcc tttgagctgc cagcggatga actgtcagaa
     7981 agtctgggga aaatcgccca gctctacaaa atacctaccc gcaatattga acagctcggc
     8041 gatgcactga actatctgga tgataacgcc atgtcgaaag gggcggacat cattgatgtg
     8101 atgcaacgtc tgggcggtgt ggctgaccgt ctggattatc gtaaagcggc ggcactgggt
     8161 tccacctttc tgacactggg cgctgcgccg gaggttgcag ccagtgcagc aaacgcgatg
     8221 gtgcgtgaat tgtccattgc caccatacaa agcaagagtt tctttgaagg gatgaatctg
     8281 ctgaaactca atcctgaagt gattgaaaag cagatgacga aggatgcgat gggaactatc
     8341 cagcgtgtgc tggagaaggt gaacgcactg ccgcaggaca agcgtctgtc tgccatgact
     8401 atgttgtttg gcaaagagtt tggcgatgac gcggcgaaac tggcaaacaa cctgccggaa
     8461 ctgcagcgtc agttaaaact gacagcgggc aatgatgcgc ttggctccat gcagaaagaa
     8521 tccgacatta acaaggattc actttctgcg cagtggttgc tggtcaaaac cggagcgcag
     8581 aacaccttca gcagcctggg cgaaacgctg cgccagccgc tgatggatat tctgtacacg
     8641 gtgaaaagcg tcacgggggc gtcgcgtcgc tgggtggaag ctaacccgga actgacaggc
     8701 acactgatga aagcatcggc tgttgtggct gcggttaccg tcggcctcgg caccttagcg
     8761 gtggcgctgg ctgcagtgct ggggccgctg gcagtcatcc gtctgggatt ctctgtgttg
     8821 ggtatcaaaa cgttatcttc cgttacggca gcagtaactc gaaccagcag cgcgttgtcc
     8881 tggctggctg gcgcaccact ggcactgctg cgacgcgggc ttgcttcatc cggcaacgcc
     8941 gcaggtttac ttactgcgcc gttgtcgtct ttgcgccgca cggcatcact gacgggaaat
     9001 gtcctgaaaa ctgtagcagg tgcgcaggtt gcacttttgc ggtctggatt atccggttta
     9061 cgtgctgttg ctgtgatgtt tatgaatcct ctggcggtac tgcgcggtgg actggccgcc
     9121 gcaggcacgg tgctgcgagt actggcatct ggtccactgg cgatgctgcg cgttgccctg
     9181 tatgccgtat ctggtctgtt aggtgctctg ctcagtccga taggtcttgt ggttactgca
     9241 ctggcgggcg tggcgctggt tgtctggaaa tactggcaac ccatcaccgc atttcttggt
     9301 ggcgtggtgg aaggattcaa agcggcggca ggtcccgtca gtgcagcatt cgaaccgctt
     9361 aagcccgtgt tccagtggat tggcgacaaa gtacaggcgt tgtggggctg gtttactgat
     9421 ctgctgacgc ccgttaagtc gacctctgcc gaactgcaga gtgcagcggc aatggggcgg
     9481 cgattcgggg aggcactggc ggaagggctg aatatggtca tgcatccgct ggactccctg
     9541 aaatccggcg tttcctggtt gctggagaag ctcggcattg tcagtaaaga ggccgcaaag
     9601 gcaaaactgc cggaaagcgt gacgcgtcag caacctgcga cggtgaatgc agacggtaaa
     9661 gtgatgacgc catcgggtgg ttttccatca tggggatatg gctttgcggg gatgtatgac
     9721 agcggcgggt atatcccgcg cgggcagttt ggcatcgtcg gtgaaaacgg gccggaaatt
     9781 gttaacggcc cggcaaatgt gaccagccgg agaaatacag ctgcactggc tgccgttgtt
     9841 gccggaatga tgggcgttgc tgccgcgcct gcagagcttc caccgttgca tcctttggca
     9901 cttcccgcga aaggcggcga agcgatggtg agtcgtgcag ccactgtgcc gctcgttcaa
     9961 cggattgagg caccgacgca gatcatcatt cagacgcagc caggacaaag tgcgcaggat
    10021 attgcgcggg aggtggcacg ccagcttgat gaacgtgaac gcaggctgaa ggcaaaggcc
    10081 aggagtaact acagcgatca ggggggatac gacgcatgat gatggtgctg ggattgtacg
    10141 tgtttatgct gcgcaccgtt ccgtatcagg aactgcagta tcaacgcagc tggcgacatg
    10201 cggcaaacag tcgggtaaac cgtcgtccgt ccacgcagtt tctgggaccg gaaaacgaca
    10261 tgctgacgct ttccggtgtt cttatgccgg aaataacagg cggcaggctg tcgttgctgg
    10321 cactggagca gatggcagaa caggggaaag catggcccct gattgaaggc agcggcatga
    10381 tttatggcat gtatgtgatt gagggactga atcagactaa aacggagttt ttccgcgatg
    10441 gtatgccgcg ccggattgag tttaccctgt cgctcaaacg ggtggatgaa tccctgtccg
    10501 atatgttcgg tgatctcagt gcgcagctga ataatttgca ggatacggca acgtctgcct
    10561 taagcgatat cagtaaaacg gtgggagggc tgctgtcgtg aatttcagct ctgaactgct
    10621 taacaaaggc aacaaaactc ccgcattcag catcagtatt gaggggaagg atatcaccac
    10681 tgtgctggat aaccgcctga tgagtctgac gttgacggac aatcggggct ttgaagcaga
    10741 ccagcttgat ctggagctgg acgacgccga cggaaaaatc gtgctgccgc gccgtggtgc
    10801 ggtcatcacg ctggcgctgg gctggaaggg gcagccgctt ttcccgaaag gggcattcac
    10861 ggtggacgag attgaacaca ccggcgcacc ggatcgcctg actatccggg cgcgaagtgc
    10921 tgattttagg gaaacgctga atacccgccg tgaaaaatcg tggcacaaga ccactgtcgg
    10981 ggaagtggtg aaggaaatag ccgcgcgtca taagctgaag atggcactgg gtaaagacct
    11041 gtcggataag cccgtggagc atatagacca gactaatgag agtgacggca gttttctgat
    11101 gcggctggcg cgccagtacg gtgccatcgc gtcggtgaaa aatggcaatc tgttattcat
    11161 ccggcaggga cagggcaaaa gcgccagcgg taaaccactg ccggtgatct caatcacacg
    11221 taaggacggc gacagtcacc gctttaccct ggcagatcgc ggagcctaca cgggcgtaat
    11281 tgccagttgg ttgcataccc gcgaacccgc gaagaaagaa agcaccacgg tgaagcgtaa
    11341 gcgcaggact aagaagcaga agaaagagcc ggaagcgaag cagggcgatt acctggtggg
    11401 tacggatgaa aacgtgctgg tacttaatcg cacttatgcc aaccggagca acgccgaacg
    11461 ggcagcgaaa atgcagtggg aacgcctgca acgcggcgtt gcatcattct cgctacaact
    11521 ggcggaaggg cgggcagatc tctacacaga aatgcctgtg aaggtcagtg gctttaaaca
    11581 gccgatagat gatgcggaat ggaccattac gactctgacg cataccgtca gccccgataa
    11641 cggttttaca accagtctgg agcttgaagt gaggattgat gatttcgaaa tggaatgatt
    11701 cttcgcaatg gagaactttt aagtttgcaa aatggaataa tgcggtatca ttattgtgaa
    11761 tttagcaaaa atggggagaa ctcgaaaaat gatgatttgc ccactgtgtg gaagtgccgc
    11821 ccatactcgc agcagttttc aggtatcttc attgaccaaa gagcgttaca accagtgcca
    11881 gaatattaac tgcagtcata cttttgtaac ccatgaaact tttgttcgtt cgattgcaac
    11941 gccaaaagag tcaaatccgg ttcagccgca tccaatgaaa tcaggacagg tggcgctctc
    12001 tctttgacgc tgccgccatt ttgtcgccat cgttaaaaaa cagtgcttct aacgtcatga
    12061 ttttaaacgg cataaatttc aggcaacaaa aaacccatca accttgaacc gaaatggcgg
    12121 ggttgatggg ctccacaaaa tggggacatc aaagaaaagc agtggcacta attaagactg
    12181 atgccctgcg gaaaagttct gcggttgtgc aaaaaaattt cattttcagg gcaacttcag
    12241 ttttatccta atcctggcca taccatgacg atgattgtcc ctgccagcgt cagcaggacg
    12301 ttggcgattg cgtaggtgcc cgcgtagccc agcgcaggga tgttactgcg agctgtatca
    12361 ctgatgatct ccattgccgg cgcgcaggtg cgtgcgccca tcattgcgcc gaacaacagt
    12421 gcgcggttca ttcgcaatac ataagcaccg aacaagaaac agataaccac gggcacaaga
    12481 ctgacaatta atccggcaat caacatctga ccgccaatcg cgcccaggcc gttattaata
    12541 ccgctaccgg cgctcagacc aacgcctgcc ataaacacca tcaagccgaa ctctttcacc
    12601 atgcttaatg caccttgcgg aatgtaaccg aaggtcgggt ggttagcacg cataaagccc
    12661 agcataattc cggcgaataa caacccggca gcgttcccca tgccgaaact gaatgtgctg
    12721 aactggaagg tgatcatccc gatcatcagc ccaataacaa agaaggcgca aaatgccagc
    12781 aggtcagtga cctggctgtg aatcgagata aagccgatgc gatcggcgat ggtttttacg
    12841 cgacgggcat caccgctgac ttgtaaaacg tcacctttgt taagcacgac gttgtcatct
    12901 atcggcatct caatctggct acgaatgacg cggttaagga agcaaccgtg atcggtcaac
    12961 ttcagttgtg cgagacgttt acctacagcg ttatggtttt taacgaccac ttcttcagtg
    13021 acgatacgca tgtcgagaag gtcacgatcg aaaacttctt taccgttacg gaagctggga
    13081 tcgagtcggg catgggcgtc gggatagcct accaacgcta tttcatcgcc catttgtagc
    13141 acggcatcac cgtctggatt tgccagaatc ccgttacgtc gaatacgttc aatgtagcag
    13201 ccggtttgtc gataaatacc cagttcacgc agatttttgc cgtcggtcca ggccaccagc
    13261 tccgggccga cgcgataggc gcggatcacc ggtaaataaa ccttacggtt ggcatcagtg
    13321 tccaggccac gttcgcgggc gatttgctgg gcgctggtct gtaagtcctg atgctgcaat
    13381 ttcggcaagt aacgcgcacc aacaatcaaa ctcaccagac cgattaaata ggttaaggca
    13441 tacccgaggc tcagattatc cagtgccagt gagagctgcc tgctttccat gccggaatga
    13501 cgcagtgtat cgccagcacc gaccagaacc ggtgtcgacg tcatagagcc tgctaacata
    13561 ccggccgtca ggccaatatc ccagccaaac agcttaccta accctaaggc gatcaccagc
    13621 gcactgccaa ccatcaccag tgctaacatt aggtaatttt tcccatcgcg aaaaaaaatg
    13681 gaaaaaaagt tcggtccggc ttcgaccccg acgcagaaaa taaacagcat aaagccaaga
    13741 ttaagcgcat cggtgttaat gctgaaatgt tgttggccta ataacagcga tacgactaaa
    13801 acgccaatgg aattacccag ttggatcgaa ccaagtcgta actttccgag acatagccca
    13861 agcgcgagga ccacaaataa taacagaatg taattcccat ttaacaattc ggcgacgttt
    13921 atattcacgg aggctaactt cttgtttact agtaagctgt tgaaagaaat ggtaatttac
    13981 gataatgttt tttaccagaa ttcagggcgc agattcattc agcgcaccta aacgatagta
    14041 aagtaacaat atattttact agtgtaatca cattaggtat caacggctat atgaattgcg
    14101 ttggcctata ttagcatgga atgcgaagcg gctttatctt actgaacgcc acactggcga
    14161 aaaatgtgtt cgatagacgc agtgtcagga ggaacgagtg aaacataaac aacgttgggc
    14221 gggggcaatc tgctgttttg tcctcttcat tgtggtgtgc ctttttctgg cgacgcacat
    14281 gaaaggcgct tttcgggctg ccgggcatcc tgaaatcggc ttgctatttt tcattcttcc
    14341 tggagcagtc gccagcttct tttcacagcg tagagaagtc ctgaaacctc tgtttggcgc
    14401 aatgctggcg gcaccctgtt cgatgctcat tatgcggctg tttttttcac cgacgcgctc
    14461 attctggcaa gagctggcat ggttactaag cgcggtgttc tggtgtgcgc tgggggcact
    14521 gtgtttctta tttatcagta gtttgtttaa accacagcac agaaaaaatc agtaaagccc
    14581 tcaacgcgag ggcttgtcag acgatcaggc gtccagattt tctttcaccc atgcagcaaa
    14641 atcggtatag ccgccgatat gttgctgatc gacaaaaatc tgcggcacgg tttctacggg
    14701 tttacctgcc ttttgttgta gatcttcttt agtgatccct tccgcacgaa tatctacata
    14761 ctgatactga aaatcatcgc gttcattgct caatttctca gccagatctt ttgcacgcac
    14821 acagtaaggg caacccggac gaccaaaaat aacggtttgc attatttctc tcctcataga
    14881 tttatgcctg taatgatcac gctaaaatgt attcgctgaa agtaggttta acctgttgca
    14941 ttaattgcta aaagctataa ctgttaaaca caatacagtg aaaagtttta gactgaaggc
    15001 tcactttgca gagggaagcg tatgcgcgcg atcggtaaat tgcctaaaag cgtgttgata
    15061 ctggaattta tcggaatgat gttgctggcg gtggcgctgc tgtcggtaag cgactccctg
    15121 tcgctgcctg agccattttc tcggccagaa gtgcagattc tgatgatttt tctcggtgtt
    15181 ttgctcatgc ttcccgctgc ggtggtggtt attcttcagg tggcaaaacg tcttgcccca
    15241 cagctgatga accgtccacc gcaatattca cgttcagaaa gagaaaaaga taatgacgcc
    15301 aaccattgaa cttatttgtg gccatcgctc cattcgccat ttcactgatg aacccatttc
    15361 cgaagcgcag cgtgaggcga ttattaacag cgcccgtgcg acgtccagtt ccagtttttt
    15421 gcagtgcagt agcattattc gcattaccga caaagcgtta cgtgaagaac tggtgacgct
    15481 gaccggcggg caaaaacacg tagcgcaagc ggcggagttc tgggtgttct gtgccgactt
    15541 taaccgccat ttacagatct gtccggatgc tcagctcggc ctggcggaac aactgttgct
    15601 cggtgtcgtt gatacggcaa tgatggcgca gaatgcatta atcgcagcgg aatcgctggg
    15661 attgggcggg gtatatatcg gcggcctgcg caataatatt gaagcggtga cgaaactgct
    15721 taaattaccg cagcatgttc tgccgctgtt tgggctgtgc cttggctggc cagcggataa
    15781 tccggatctt aagccgcgtt taccggcctc cattttggtg catgaaaaca gctatcaacc
    15841 gctggataaa ggcgcactgg ctcagtatga cgagcaactg gcggaatatt acctcacccg
    15901 tggcagcaat aatcgccggg atacctggag cgatcatatc cgccgaacaa tcattaaaga
    15961 aagccgccca tttattctgg attatttgca caaacagggt tgggcgacgc gctaaaaccg
    16021 ccacgtcgat gtatgatacg cgggcttttg accaggtctg acagagaggt gcagggtgaa
    16081 aattgccata ttgtcccggg atggaacgct ctattcgtgt aagcggctgc gtgaagccgc
    16141 tatacagcgc ggtcacctgg ttgaaattct tgatccgctt tcttgctaca tgaacataaa
    16201 tcctgcggcg tcttctattc actacaaagg ccgcaagtta ccccattttg acgcagtgat
    16261 cccgcgtatt ggcaccgcca ttacctttta tgggacggcg gcactgcgcc agttcgagat
    16321 gctggggagc tatccgctca atgagtcggt cgccattgcc cgggcgcgtg acaaattgcg
    16381 ttccatgcaa ctgctggcgc gtcagggcat cgacctgcct gtcacgggca ttgcgcattc
    16441 gccggatgat accagcgatt taatcgacat ggtcggtggt gcgccgctgg tggtcaagct
    16501 ggttgaaggc acgcagggaa ttggcgtcgt gttggcggag acgcgtcagg cggcggaaag
    16561 cgtgattgac gctttccgcg gtctgaacgc gcatattctg gtgcaggaat atatcaaaga
    16621 ggcgcaaggg tgcgatatcc gctgtctggt tgttggtgat gaagtggtcg ctgcgattga
    16681 acggcgagcg aaagagggcg attttcgttc caatttgcat cgtggcggcg cggcaagcgt
    16741 cgccagtatc acaccacagg agcgtgaaat cgcgataaaa gccgcgcgaa cgatggcgct
    16801 ggacgttgct ggtgtggata ttctgcgtgc taatcgcggg ccgttggtga tggaagtgaa
    16861 tgcgtcgcca gggctggaag gaatagaaaa aacaaccggt atcgacatcg cgggtaaaat
    16921 gatccgctgg atcgaacgcc acgctacgac ggaatattgc ctgaaaacgg gtggttagtc
    16981 gcaatcacat tactgatcat ggttttgcct gcgctttttg cgtaagctgt gccggtcttt
    17041 ttatcggaag aggttgtaca aaattatgac atcgctggtc gttcctggtc tggatacgtt
    17101 gcgtcaatgg ctcgatgacc tggggatgag tttttttgaa tgtgataact gtcaggctct
    17161 gcatctgccc catatgcaga atttcgacgg tgtctttgat gccaaaatcg atctgatcga
    17221 taacacgatc ctgttttctg ccatggcgga agtccgacct tcagccgtat tgccgctggc
    17281 ggcggattta tctgccatca atgctagttc gctgaccgtg aaagcatttc ttgatatgca
    17341 ggatgataat ctgccaaagc tggtggtttg ccagtcttta tccgttatgc agggcgtaac
    17401 ctatgagcag tttgcatggt tcgtgcgtca gagcgaagag cagatttcga tggtcattct
    17461 tgaagctaat gcccatcaac tgctgttacc gactgacgat gaagggcaaa acaacgttac
    17521 cgaaaactat ttcctccact gataactcct ttcgagcacg cagtcgctgg tgcagtggct
    17581 gcgcgctgca aaattatctg ctgtttttaa ccttttctta aagattgttt cacttatctt
    17641 gtgtcgattt ggctttatca catagagcaa atacgcataa aaatttgtta aataccgttt
    17701 tttaatccga gctatagtct caaaccctgg ctaaagttat tcttgcgatg cttttatata
    17761 gcgagcagtg ctggccggga gagagttctc ttttcttaca ccgcgccgat aaaaaatatg
    17821 cacgtttatt gcatatcttt cagtgtgaca acttttgttc gtttgttaac gaactttcag
    17881 aaggaaagag atatgaccgc cttaaataaa aaatggctat cgggtctggt tgcgggtgct
    17941 ctgatggccg tctctgtcag cacgctcgcg gctgaacaaa aaacactcca catttataac
    18001 tggtctgatt atatcgcccc ggacacggtg gccaattttg aaaaagaaac cggtattaaa
    18061 gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat ggcggggagc
    18121 accggctttg acctggtggt gccgtcagca agctttcttg aacgccagct gacagcggga
    18181 gttttccagc cgctggacaa aagcaaattg ccggagtgga agaatctcga cccggaactg
    18241 ctgaagttgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta tatgtgggcg
    18301 acgaccggga ttggctataa cgtcgataaa gtcaaagcgg tgctgggcga aaacgcgccc
    18361 gtcgatagct gggacttgat cctcaagcct gaaaatctgg aaaaactgaa aagctgtggc
    18421 gtctctttcc tggatgcgcc tgaagaagtt tttgctaccg tgctgaatta tctcggcaaa
    18481 gatcccaaca gcactaaagc agatgattac accggaccgg caaccgatct gctgttaaag
    18541 ctgcgcccga acattcgtta tttccattca tcgcaataca ttaacgacct ggcaaacggc
    18601 gatatttgcg tcgctatcgg ctgggcaggc gatgtctggc aggcgtcaaa ccgcgcgaag
    18661 gaagcgaaga atggcgtgaa tgtctctttc tcgattccga aagaaggggc gatggcgttc
    18721 tttgatgtct tcgccatgcc tgcggatgcc aaaaacaaag acgaagccta tcagttcctg
    18781 aattacctgc tgcgcccgga tgtagtagcg catatttccg accatgtgtt ctatgccaac
    18841 gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc gggtatttat
    18901 ccgcctgcgg atgttcgtgc gaagctgttc actctgaaag tgcaggaccc gaaaatcgac
    18961 cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc agccttagat
    19021 gccgggcggg cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat
    19081 tcaatgccgg agagctgccg tgaatgacgc tatccctcgc ccccaggcga aaacccgtaa
    19141 ggcgctgacg ccgctattag aaatccgcaa cctgaccaaa tcctacgatg gccaacatgc
    19201 ggtggatgat gtcagcctga ccatctacaa aggtgaaatc ttcgcgctac tgggcgcatc
    19261 cggctgtggc aagtccacgc tgctgcgtat gctggcaggt tttgaacaac cttctgccgg
    19321 acagataatg cttgatggcg tcgatttgtc acaggttccg ccttacctgc gccccatcaa
    19381 tatgatgttt cagtcttacg cgctgtttcc gcatatgacc gtggaacaga acatcgcttt
    19441 tggcctgaaa caggacaaac taccgaaagc ggaaattgtc agccgggtca atgagatgct
    19501 cggcctggtg catatgcagg agttcgccaa acgcaaaccg catcagcttt ccggtggtca
    19561 gcgacagcgt gtggcgctgg cccgaagcct tgccaagcgc ccgaaactat tactgctcga
    19621 cgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga
    19681 tattctggag cgtgtcggcg tgacctgtgt gatggtcact cacgatcagg aagaggcgat
    19741 gacgatggcg ggacgtatcg ccattatgaa tcgcgggaaa tttgtccaga ttggcgagcc
    19801 agaagagatc tacgaacatc caaccacccg ctacagtgct gaatttatcg gttcggttaa
    19861 tgtctttgaa ggtgtactca aagagcgtca tgaggatggg cttgtgcttt attcgcctgg
    19921 gctggtgcat ccactgaaag tcgacgcgga tgcctcggtg gtcgataacg tgccggtaca
    19981 tgtggcgctg cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa
    20041 cttcgcggtg ggggaggtga tacacattgc ctatctcggc gatctttcgg tgtatcacgt
    20101 tcgtctgaaa agtgggcaga tgattagcgc ccagctacaa aacgcccatc gtcatcgtaa
    20161 agggttaccg acctggggcg acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt
    20221 gctgacggtt taaggagcaa agatgaatac acttgaacct gctgcccagt cgaaaccgcc
    20281 gggcggattt aagctgtggt tgtcgcagct gcaaatgaag catgggcgca aactggtcat
    20341 tgcgttgcca tatatctggt tgatcttgct gtttctgctg ccatttctga ttgtctttaa
    20401 aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga tggagtgggc
    20461 tgacgggcaa ctgtccatca ctcttaatct cggtaatttt ctgcaactga ccgacgatcc
    20521 gctctatttc gatgcttatc tccagtcgct acaggtggct gcgatttcga cattttgctg
    20581 tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa
    20641 tattttatta ctactggtga tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc
    20701 ctggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt ggctgggggt
    20761 tatcgatcaa ccgctgacca ttcttcatac caatctggca gtttatattg gcattgttta
    20821 cgcctacgtg ccgtttatgg tactgccgat ttataccgcg ttgattcgta ttgattattc
    20881 gctggtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct ttactgtgat
    20941 cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta tcccggctgt
    21001 gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga tcgggcgcgt
    21061 gctatggcaa gagtttttca ataaccgcga ctggccggtg gcctcggcgg tagcgatcat
    21121 catgttgctg ctgctaattg tgccgataat gtggttccac aaacaccagc aaaaaagcgt
    21181 gggagaacac ggatgaataa tttaccggta gttcgttcac cctggcggat tgtgattttg
    21241 ctgctgggct ttacctttct ctacgcgcca atgctgatgc tggttatcta ttcgtttaac
    21301 agctcgaagc tggtgacggt gtgggccggc tggtcaacgc gctggtatgg tgagttattg
    21361 cgcgatgacg cgatgatgag tgcggttggt ttaagcctga caattgctgc ctgtgcggca
    21421 acggcggcgg cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt tggcagattt
    21481 cgcggatcaa atggctttgc ctttatgatc accgcgccgc tggtgatgcc agatgtcatc
    21541 acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg ctggcctgcg
    21601 gaccgcggta tgctcaccat ctggctggcg catgtcactt tttgtacggc ttatgtggcg
    21661 gtcgttattt cttcaagatt gcgggaactg gatcgctcga tagaagaagc agcgatggat
    21721 ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat catgcccgcg
    21781 atcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt gatcgccagc
    21841 tttgtttccg ggccgggagc caccacgtta ccgatgctgg tcttttccag cgtgcggatg
    21901 ggggtgaatc cggaaatcaa cgcccttgca acgttaattc tcggtgcggt cggaattgtc
    21961 ggatttatcg cctggtatct gatggctcgc gcagaaaaac agcggatacg cgatatccag
    22021 cgtgcaagac atggctgaag acactaaaat ttgccaacct ggctacataa tgccgcgcat
    22081 gccgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa acatcttcat
    22141 ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc attattatga
    22201 tccgtggcct cgacgtgctg atgattttca atacgctggg cgtgcgcggt attggcgagt
    22261 tcattcatcg cagcgtacaa acctggagtt taacgctggt ctttttaagc agtctggtgc
    22321 tggttttcat tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct
    22381 ggctatatct gctgacacaa atcaccgccg caagttactt gtgggcggct tcgctggggt
    22441 acggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc
    22501 tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt ccctcgacca
    22561 gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg atgtgaaggt
    22621 caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg
    22681 cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct gctcgccgat
    22741 tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa
    22801 gccaaaatgg tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct gcatcgcgac
    22861 ggcacaccag aagacctttg tgactgcccg ctttatcctg cctcatttgc gcccgttttt
    22921 gcggcgctaa aaccgtttat cgcccgtgcg gggctgacac cctacaacgt ggcgcgtaag
    22981 cgtggcgaac tgaaatacat tctgctgact gaaagccaga gcgatggcgg catgatgctg
    23041 cgctttgtac tgcgttctga aaccaaactg gcacaactgc gtaaggcgct gccgtggtta
    23101 caggaacaac taccgcaact gaaagttatt accgtcaata ttcagccggt acatatggcg
    23161 attatggaag gggagacgga gatctacctt actgaacaac aggcgctggc agagcgtttt
    23221 aatgacgtac cgctgtggat ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc
    23281 agccagctgt acgctaccgc gcgcgactgg gtacggcagc tgccagttaa acatatgtgg
    23341 gatctgttct gcggtgtggg tggctttggt ttacactgcg cgacgcctga catgcagtta
    23401 accgggatcg aaattgcatc agaggccatt gcctgtgcaa agcagtcagc cgctgaactg
    23461 ggcttaacgc gtttgcaatt tcaggcgctg gactccactc agtttgccac cgctcagggg
    23521 gaggtgccgg agctggtgct ggttaatccg ccgcgccgcg gcattggtaa accgctatgt
    23581 gattatctct caacaatggc accgcgtttt attatctact ccagctgtaa cgcccaaacc
    23641 atggcgaaag atatccgcga actgccaggt taccgcatcg aacgggtaca gctttttgat
    23701 atgttcccgc acaccgcgca ctatgaagtg ctgacgctgc tggtgaagca ataaaaaagc
    23761 cgcaggtgcg gcttcagatt gctgatcagg tgcgctttgt tcatgccgga tgcggcgtgt
    23821 acgccttatc cggcctacaa aatcatgcta aatcaataca ttgcagagtt gatgtgggcc
    23881 ggataagcgc agcgcatcag gcaatcttac ttttgtcatc agtctcaatt actgcggaaa
    23941 ccactggtca ttgattttct gatatgtacc gtcagcttta attgctgcca gcgcgttatt
    24001 cagtttttcc agcagagctt tgttatccgg acgtacagcg atgcccagac cggtgccgaa
    24061 gtattgcgga tcggtcactt tttcagtagc aacgcccagt tgcggattgg ttttcagcca
    24121 ttcgtttacc accgctgtgt caccaaatac cccatcaata cgaccatttt tcagatcgat
    24181 aaaggcattc tgataactgt cataggagac agttttcact tcggggtgct gatcctgaat
    24241 atatttctgg tgcgtggtgc cgttttccat cccaatacgt ttgcctttca gatcggcaaa
    24301 cgttttgtag gtatcttttt tggcaatcac gacggctgag ttttcatagt agggcgtggt
    24361 aaacgatacc tgcttgctac gctcgggcgt gatatccata ccggagatta cggcgtcata
    24421 ttttctgaat ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa aagtacattc
    24481 tgcctgcatt tgtttgcaca aggcttttgc cagatcgata tcaaagccga caatctcatt
    24541 attagcacct atagattcaa agggtggata ggtggctgaa acgccaaaat tgattttctc
    24601 tgcggcagaa gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta actttttcat
    24661 gatggaactc ccgtctgtca atcttatgat ttttggccgt gtctgcggca tgggataaca
    24721 atgccatcaa gtgaatttat atgcaataaa tatgattaaa taatttaaat gaaataaaaa
    24781 aagacggata ccttagtgag ttgtccgtct tcattataag aatttatgca ctatgtaggc
    24841 cggataaggc gtccacgccg catccggcac aggcaccgtg ctgatgtctg atgcgacgct
    24901 ggcgcgtctt atcagaccta caaacccccc cggcgaatgt acgcagctac attaattccg
    24961 ccgttcgaat gccagtgctt tgcgctcgat cagacgcatc atcagcgtca gcaggccgtt
    25021 gacgaccagg taaataatcc ctgccgcacc gaacaccatt acatcgtagg tgcgtccgta
    25081 caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca gagaggtact
    25141 tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa aggcatacgg
    25201 cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg actgccactg
    25261 accttctggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt
    25321 cagcgataac gcaatcagcg cacataacca cggttctgac aacaaatgcc acagtgccgg
    25381 atactcctgc aaagtcggaa actggcccgg cccgtaataa atcaggaaga tctgcaccag
    25441 cagcggcgta ccggtaaaca gcgtgatata accccgcacc agccacacca gcaccggcgt
    25501 tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc
    25561 aacggttagc gtcaggctgg tgtgtagccc tttcatcagt tcgggtaaat actcaaacat
    25621 tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga
    25681 ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt aaatggttcc
    25741 tgggtacgag tagcgatgct ttttgtttgc agcattaaat cattcacact aatcaaactg
    25801 accagcgcgg tatctttcag cagcaccagc cactggttac cgaggccagg cagcgcatga
    25861 cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag ccccagcgcc
    25921 tgaccagact cccactgacc caccggcacc gctttcaacg cgccccgaag cgtttgcgag
    25981 gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg gctaacgtcg
    26041 aagttctcaa tgtccatctg cactgggatc tgcacgaacc caagattgat agtgaagcca
    26101 tccgaaagcg tcagcagcag ctgcgaggag ccaaaataga taaacagcac caccagaatt
    26161 tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac aggacgccat
    26221 tttgccgact cccataccgc aaagaacatc gccagcgcca gcccgacaat caatgcacaa
    26281 acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc attcatcagg
    26341 aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt cttcactttt
    26401 tccagcgcag tgttgagttt ctgctgcagc tcagtgttgc cctgacgtac cgcgatgccg
    26461 aggccagtgc cgaagtaatc tttatcggtc actttgtcgc ccaccgccgc cagtttcggg
    26521 ttatctttca gccactcagt gaccactgcg gtgtcaccga agacgccgtc gatacgcccg
    26581 ttttgcagat ccagttttgc gttctggtag ctgtcatacg gaacggtagt gatttccggg
    26641 tgcttatcca taatgaattt ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct
    26701 ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc agagttgtca
    26761 tagtacgggg tggtaaacag tacctgcttt tcacgctccg gagtgatatc catgcccgcc
    26821 atcactgctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa cgcctggtta
    26881 gagaaagtac aggttgcatc aatctcttta cacagtgcct gtgccaggtc gacgtcaaaa
    26941 ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc ttcggtagca
    27001 aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc
    27061 agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt ttaaatgctt
    27121 cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga ccattttcca
    27181 tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg acgatcacct
    27241 gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc gtaatttccg
    27301 ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc atcaacgcac
    27361 gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa cgatcgctat
    27421 aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc tgatctttac
    27481 tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc
    27541 acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga atcgctttgt
    27601 cagagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg
    27661 gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg ctggggccaa
    27721 gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg
    27781 cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt atactggcag
    27841 tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc aatatttctg
    27901 ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat aacgggggcg
    27961 gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt tacggggttt
    28021 attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc cgccgtggca
    28081 tgaacgctac cgcctaagta acgcacatcg tcgatgaccc aacactggcc ttcctgaatc
    28141 attaacactt catcctgcca accctggtca ccctgtttga gatccacgcg caatggaatg
    28201 ttacgggcat cacgattagg gatagtcgat gcactggcaa cgtgggcgct atctggcaag
    28261 gtggttcgac tggagaatgg atcgttggtc agtagttccc gatggttatt atcccgggag
    28321 gcatcgctaa gcagtgtcgc cagtttgtcg ctcagataag ggcgcaaggc ggtgatgtcg
    28381 ttgctgcggt gcaaaatgcg gtagtcataa aattgctggg ccacgttatc cgggcctcct
    28441 tcaacgcaag gaccactgcg tgtgccgtta tctttataag ctggagtgac tgtggtgcag
    28501 gcactgagga gcagtgcgca ggggataagc attgtcaatt tgctgtagcg cataatgatt
    28561 tccttataag cgatcgctct gaaagcgttc tacgataata atgatatcct ttcaataata
    28621 gcgtatcagt ctgataatgc ttttgagatc gaaggcttag caaacaagga gatcgatcat
    28681 gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt gcggtgtggt
    28741 gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgccg gtatccgcga
    28801 tatcgttggc ggacgttccg gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc
    28861 ctttgaggaa ttaggctccc aggcgcgggc gctgggggcc gatgccgtcg tcggtattga
    28921 tatcgactac gaaacggtcg ggcaaaacgg cagtatgctg atggttagcg tcagcggtac
    28981 ggcggtgaaa acgcgtcgat gagaagagtc ttctggctga tcgctgccgc tctgttactg
    29041 gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct tgatacccga
    29101 cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta caccgcagat
    29161 gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca ttatctggtc
    29221 cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt gccggaacaa
    29281 gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct taacgacacc
    29341 tctattggca ttgagctgga aaaccgtggc tggcaaaaat cggccggagt gaaatatttt
    29401 gccccgtttg aaccggcaca gattcaggcg cttattccac tggcgaaaga tattattgcc
    29461 cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa
    29521 gacgatccgg ggccattatt tccctggcag caactggcgc agcaggggat tggtgcctgg
    29581 ccggatgcgc agcgggttaa cttttacctt gccgggcgcg cgccgcacac accggtagat
    29641 accgcgtcat tgctggagct tttggcgcgc tacggttatg acgttaaacc tgatatgaca
    29701 ccgcgcgagc agcggcgcgt gattatggca ttccagatgc atttccgccc gacgttatat
    29761 aacggcgaag cggatgcaga aactcaggcg attgccgaag cattgctgga gaaatacggg
    29821 caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt ctcgatacct
    29881 tcatccaggg tgatgaccgg ctgataacct aactcttcct gcgcacgcgt aatatccagc
    29941 gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc ttttgctgac
    30001 ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta ggggacggaa
    30061 cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct gcgcagtgtg
    30121 cgatgctcgc cgttggtgat gttgtacaca cgcccggaag gtagcttatc gcaggcttcc
    30181 tggcttgcca gccacattgc gtgcacggca ttttcatagt aggtcatatc gaccagcgca
    30241 ctgccgccat gcggtaacag aatactgccg tagtggtgca tcatatgcgc cagacgggga
    30301 ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt
    30361 tgtggattcg cctgcgaaag catattgatc acttcttcgc tggctgcttt gctgcgggca
    30421 aactcgttgg cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa
    30481 tcgaagtaca gggaggggga agagatatga ataaagttac gcacacccca ggcgacagcc
    30541 cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc
    30601 cagggtgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag catcacttta
    30661 gcttgtgatg aaaccagctc ggtcagatcc gccggaacaa actctgcgcc cattttttcc
    30721 agcaatttgc ccattgcctc gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat
    30781 aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag taccttcata
    30841 tcaatccact gttgttgaga aaataacgtg cgcattcttc cgtgatttcc cccatgatgc
    30901 aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca
    30961 gtctggcgat ttgttttgcc attccgcgaa aaataaacag atgcgcgggg atcatcaata
    31021 accagtaaaa cagccccggc ataccgtgcg gatgccagaa agcgcggaca tcgatagtac
    31081 gatagtcgcc tttatcttcc aggctaaaac acagtcgtcc cagccccggc gctttcatgc
    31141 caaataacaa cgtaagttgt ttttccggtt caacgacaat cactttccag ctatctaccg
    31201 catcgccagt ctgtaaatat tcgcgctccg ggcggccttt cgccagctta tgaccgatcg
    31261 cgcggtccat caacgcccgt gtctgccaca aaatattgcc aaagaaataa cgctctttac
    31321 cgccgatttg gttcactacc tgccataaag cagcaaggct ggcggacgtt ttaacggtaa
    31381 accccgcctg tttggcaaaa taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt
    31441 cgtagcccca gtcgctggag ttgaccagtt tttcctcctc tttcaacgtg ctacgtaccg
    31501 cgtcatcgaa agcgatcagc cgttgtggga tgagtgcacg tagcgcggta tcatccgcca
    31561 gcagatcgtg tttcagcccc tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa
    31621 tcacattgag aaaccacacc gaaatccagc gggtggggag ggggatgggg atcaaccagc
    31681 ggcgcttacc gctcaccgcc ataaaatgtt caaactgttg ctgataactg agcacctctg
    31741 gtccggcggc ttcgaagatg cggtgttcgc tggccggatg atctaacaac gccaccagat
    31801 agtgcagcaa gttttccagc gcgatgggcg tggtgcgtga acgtacccag cgtggcggcg
    31861 ttaacactgg caggttgtag accatatcgc gcatgacttc gaacgccgct gaacctgcgc
    31921 caacgataat tccggcccga agttcggtca caggtacatt cgcttcacga agaatgtccg
    31981 ccgtagcctg acgagcacgc agatgatccg actgctcatg tggcggggcc tgcaacgaac
    32041 tgagaaagat taattgctta actggtactt cacgtagcgc atcgcggacg ttgagagcca
    32101 cctggcgctc ctgagcgata aaatcgccgc cttcgcccat gctgtgcacc agaaaataga
    32161 cggtatcgat atcctgcaac agggccggaa ggttatccgg ccagctgaga tcgactttat
    32221 ggcaactgac gtttgccagt tgcagctttg caagcctgtc gacatgacgt gccgccgcca
    32281 ggatctgatg cccttgctgg ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg
    32341 caccgagaac taaaatgcgt tgcggcacgt ctctctcctt aacgcgccag gaatgcgcgc
    32401 cagtgggcgg cgacttccgc cagttgttcg cgcgagacgt caagatgcgt caccaggcgg
    32461 acaatcggcg aggcgttaat cagcacgttt ctcgctttca tgtattcgcc taacgcggca
    32521 gcattttctt ccccgacgcg aacaaacagc atattagtgt cctgacgcat cacatccgcg
    32581 cctgcttcac gcagttgctc cgccatccag gcggcgttgt cgtgatcttc ctgcaaccgt
    32641 gcgacgttat ttttcagggc atacatcccg gcggcagcca gaatcccgga ctgacgcatc
    32701 ccgccaccgg ccattttccg ccagcggatg gcacgtttaa tgtaatcacg attaccgacg
    32761 agtaatgaac cgactggcgt cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa
    32821 tattgcgtga tctctttcag ttcgcagccg taagccacca cggcattaaa gatgcgcgca
    32881 ccgtcaacat gcagcgccag attgcgctcg cgggtaaatt cccatgcttc tttcaggtat
    32941 tcacgcggca gcactttgcc gttgtgggtg ttttccagac tgagtaattt ggtgcgggcg
    33001 aaatggatat cgtcgggttt gattttcatc gccactttat ccagcggtag cgtgccgtcg
    33061 gcagccgcgt cgatgggttg cggctgaata ctgcccagca ccgctgcgcc accggcttca
    33121 aacagatagt tatgcgcggc ctgaccgaca atatactctt cgccacgttc gcagtgactg
    33181 agcagagcga ccaggttggc ctgagtgccg gtaggcagaa aaatggcggc ttctttaccg
    33241 gaaagctctg ctgcatagtc ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg
    33301 tccccaaccg gggcggccat catcgcttcg agcatggcgc ggctcggtcg ggtaacggta
    33361 tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca ccctttttac
    33421 cttagccagt tcgttttcgc cagttcgatc acttcatcgc cgcgtccgct gatgattgcg
    33481 cgcaacatat acaagctaaa tcctttggcc tgttcgagtt tgatctgcgg tggaatggct
    33541 aactcttctt tggcgaccac cacatccacc aacaccggac cgtcgataga gaaggcgcgt
    33601 tgcagggctt catcaacttc agacgctttt tctacacgga tacccgtaat gccgcacgct
    33661 tcggcaatgc gggcaaagtt tgtgtcgtgt agttcggtgc cgtcagtcaa atagccacca
    33721 gctttcatct ccatcgccac aaagcccagc acgctgttgt taaagacgac aattttcact
    33781 ggcagtttca tctgcactac tgagaggaaa tcgcccatca acatgctaaa accgccatcg
    33841 ccgcacatgg cgaccacctg acgttctggc tctgtcgcct gcgcacccag cgcctgcggc
    33901 atggcgttag ccatcgaacc gtggttaaac gaacctaaca ggcgacgctt gccgttcatt
    33961 ttcagataac gtgccgccca taccgttggc gtgccgacgt cacaggtaaa gatggcgtca
    34021 tcggcggcaa aatgactaat ttgttgcgcc agatattgcg ggtgaatggc tttctcgctc
    34081 ggtttagcta aatcgtccag ccctttgcgg gcgtcgcggt aatcttccag cgctttatcc
    34141 agaaacttgc gatcggtttt ttcttccacc agtggcagca atgcccgcag ggttgatttg
    34201 atatcgccga ccagcgccat atccaccttg ctgtgagcgc cgatgctggc tgggttgata
    34261 tcaatctgaa tgattttagc atcggtcggg tagaaggcgc ggtagggaaa ttgcgtgccg
    34321 agcagcacta acgtatcggc attcatcatg gtatggaaac ctgacgagaa gccgattaat
    34381 cccgtcattc cgacatcata cggattatcg tattcggcat gctctttacc gcgcagggca
    34441 tgaacaatag gcgctttaat tttcccggca aactcaacta actctttatg cgctcccgcg
    34501 cagccgctgc cacacatcag ggcgatattg ctggaataac gcagcagttg cgccagtttg
    34561 cgtaactctt cttcttccgg cgtcacgact ggctgtggcg catgatacca gtgggtagtt
    34621 gccccttctg gcgcaggttt taacgccacg tcgcctggta acacgacaac cgaaacgcca
    34681 cggttaagca ccgctttgcg catggcaatc gccagtactt gtgggatctg ctccgggctg
    34741 gaaaccagct cgcaatagtg gctacattcg cggaataact cttgcgggtg ggtttcctgg
    34801 aaatagccgc tgccaatttc gctggaggga atatgagcgg caatagccag taccggaacg
    34861 tgattgcggt ggcaatcgaa gaggccgttg attaagtgca ggttgccggg gccgcacgat
    34921 ccggcacaga ccgccagttc tcctgaaagt tgtgcttcag cgccagcggc aaacgccgcc
    34981 acttcttcat ggcgggtgga catccactcg atggtgccca tgcgattaag actgtcacta
    35041 agaccgttca gggagtcgcc tgtgactccc cagatgcgtt tcacgcctgc cgattcgagt
    35101 gttttggcga tataagctgc aaccgtttgt ttcatggttc tccatctcct gaatgtgata
    35161 acggtaacaa gtttagttca tctgacggag ggggaaggga tgggagagaa aggaggcact
    35221 aacggttaaa tagcccgatg acaggaatat catcgggcat aaggcgatta tgcgagaacc
    35281 aaatccccct gcggatggca ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg
    35341 gtcagcgtca ttgtgctgct caccgtatat tcaccggaaa ccactttcgt cttacagcag
    35401 ccgcaaacac ccgcacggca ggcagcgaca accggaacgt tattgctttc caacgcctcc
    35461 agtagcgtgg tgccaaccgg ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc
    35521 agaccgctgg tcgccgcttc cgctactggg gtgaagaatt tctctttaaa gaaacgcgtc
    35581 acgccgagcg ctttcacttc ctgctctacc caatccatat acggagccgg gccgcaggtc
    35641 atcacggtac gtgaagctaa gtcaggaacg cgagtcagca gttcgcgagt gagacgacca
    35701 gcgataaagc cttcggtaac gttattttcc gccaccagcg ttaccggata gttacgccac
    35761 tcatcggcga aaataacatc ctgcggcgta cgcacgttgt agatcacctg tacatcggcc
    35821 tgtggacgat tcttcgccag ccagcgacgc atcgacataa tcggcgtgac gccgcagcct
    35881 gccgccagca gcaggaattt atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg
    35941 tccgaaagcc agagataatc accgcgtttt acatcgcgcg tcagccactg ggagccgaca
    36001 ccgtcatcaa tccgccgcac ggtcagggtg atgtattcac tcacgcctgg cgtggaggaa
    36061 atggtgtaag cacgcagcgt ttccgctgag tttcgtacgc tgaccagcgc atattgcccg
    36121 gcgcgatatg ggtagtaatc gtggcaaatc agggaaatcg tccacacatc cggcgtttct
    36181 tgcgtaatgt gatgaacctg catccgccac gggcattgat tcgttggcat cgtcatcgac
    36241 aaactcctta cgcgctcaac agttgcttca tgtcttcttc aacagtggtg atagaacgca
    36301 ggccgaattt ctcgttcagc accgccagca ggtctggtgt caggaaacca ggtgcagtcg
    36361 ggccggtgac gatatttttc acgcccagag aaagcagcgt cagcagaatg acgatcgctt
    36421 tctgttcaaa ccaggagagc accagcgaca gcggcagatc gttgacaccg cagcccagtt
    36481 tctctgccag agtgacagcc agaataatcg ctgagtaagc atcgttacat tgacctgcat
    36541 ctaccagacg cggcagacct tcgatatcgc caaactcaag tttgttaaag cgatatttac
    36601 cacaggcgag ggtcaggatc aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt
    36661 agtggcgctc gccgcgtgcg ccgtcacagc caccaagcag gaagatatga cgcagttttt
    36721 cacggctcac cagatcaatc agcgtatccg ctgcgccaag cagcgtctgg cggccaaagc
    36781 ccacggtgat caggtgcgga atttcgctgt acgggaagcc cgccatttgt tgcgcctggg
    36841 cgatgaccgc agaaaaatct tcgccatcca gatgacgcac acccggccag ccaacgatgc
    36901 tgcgggtcca gatacgatcg tcataagcgc ctacggttgg gtcgatgatg cagttcgagg
    36961 tcatcacgat ggggccaggg aaacgagcga actccacttg ctgattctgc cagccgctgc
    37021 cgtagttacc gaccagatgc ttgaatttac gcagctccgg gtagccatgc gcaggcagca
    37081 tttcgccgtg ggtgtaaaca ttaacgcccg tgccttcggt ctgttccagc aggttgtaga
    37141 gatctttgag atcgtgaccg gaaatcagga tgcatttacc cgccgtcgct ttgacgttga
    37201 cctgggttgg cgtcgggtgt ccgtatttac cggtttcgcc tgcatccaga atgctcatca
    37261 ctttgaagtt catctggccg atttccattg aacactcaag aagcgcgttc atatcggcag
    37321 gccaggtccc cagccacgcc atgattttat gatactgggc ataaatatcg ttgtcgtatt
    37381 gaccgagaac atgcgcgtgt tccatatagg ccgccgcacc tttcaggcca tacaggcaca
    37441 gcagacgcag gccgagaatg ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt
    37501 ctgctgcctg acgttgcagc tcgccgagat cgtcgctcac cagttgcagg tcagccatcg
    37561 ggttatcgac gcgcgcgttg gcatctacag ccaggcattg cgctttcagc gcctcgcgca
    37621 gggcaatcgc ttcacgagcg tagccgacaa tacgcggaga atcgaagtta acgttggtca
    37681 gggttgagaa aaaggcacgt ggcgcgaagc tgtcaacatc gtggttgatg atgccgtatt
    37741 cacgcgcttt taccgcccag gcagaaagcc cttgcagcgc cgcgatgagt aaatcctgaa
    37801 ggtcagaagt ttccgccgtt ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg
    37861 ccggagtacg gatagtttgt tcacattgca cacaaaacat gatcacacct tttaaagtta
    37921 tatttaatat acatgtttaa ggttaagacg cttaacgcgg ggataaaagg gatttttcat
    37981 gcaactttaa gggagattga tttagcgcaa ttttggcggc agggatctac cgccagagag
    38041 gtattacgca gagaaaaagg cgatgaggat cggcactaac aggctaagaa taaaaccgtg
    38101 aacaattgcc gccgggacca tatccagccc gccagtacgt tgaagaacgg gcagggtgaa
    38161 atccattgat gtggcaccgc ataagcccag tgcagtagag cggctgcggc gaatcagccc
    38221 agggatcaac ataatagcaa tcagttcacg ggccagatca ttaaaaaacg ccgcgctccc
    38281 gattaccgga ccaaaagatt cggtcaataa aataccggaa agagaatacc agccgaaacc
    38341 ggaggccatt gccagcgcgg tattgatggg gagatcaaga ataaaggcgt taattaaacc
    38401 accaattaat gaactgacaa ccaccaccac ggcgacaatc attccccggc gattaaggac
    38461 aatctgcttt aaggtcatgc cattattgcg caactgaata ccaacgagga aaagtagcaa
    38521 aattaacgtg tattcactgg cttcggtcgc gtgttgtaag aaagccagtc cactcagacc
    38581 aatggcaaaa ccaatcacta ctacgccgca cagttttagc gactccagcg ccatcgcaat
    38641 acgcgacggg agtttttctt gctgatggtg gttgcgccac ggcaggcctc gctccagcca
    38701 catcagggcg gcaatattac acagtaaaat aacggtaata ctgacggcag aataatgcag
    38761 aatcgccaac aggttactgg cgaggttatc gagaaacgcc agactgatac ccataaaaaa
    38821 gagaataagg taaaccatcc agcttaatag ctgattaata acttttaacg cagcttgttg
    38881 gcgaagcgga atgaggtaac ccacaatcag gggaaccaga atgattaaca gcccagaaaa
    38941 catgaaaatc cagtccttgc aaagatgaag tcgaaatgcg cgatgacaca ctactgaaag
    39001 cggaaggacg agtaaagttg caattaaaag gaaatgttat gcataaggag cagtagagta
    39061 ttcgttttca tttaaagata ttcttgcgct ttaattacaa actgcaccga tgttggtggc
    39121 gtcaaaatcg ccgaggcgtt ccctgaaggc cggggcagcc cacatggatg tgggctgagg
    39181 gcgcgtttta cagggatgtt acctcgcgcc cgacccggta gccgtaaggg ataagtcgag
    39241 ggcaccgcgc agcggcgatt ttgttcgcca gagcccgggg gtgcatgggg cggcggcgat
    39301 tggccgcccc ctgcgcgctc cttgcgccag tggcaatatg tggcttagct catgaaagga
    39361 gcgcaacaag atgatgaatc aacatataac aacatcttaa aaaaaggcct gacattacgc
    39421 caggccttct gcgttaatta atcacgcttt tccagcaggg tccggtaaat cagaccaccg
    39481 ataatgccgc cgacaattgg caccacccag aagaaccaca gttgttctaa tgcccagccg
    39541 ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga
    39601 atactaatta agtgaatcag ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc
    39661 ggcgcgaatt tgtcggttgc gccgtggatc accaacagga aacctgcact caataccagt
    39721 tcaactacca gcgcggaaag catggaataa ccgcctggtg aatgctcgcc ataaccgtta
    39781 gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac cactggcaat taaatacagc
    39841 agcgccgctg caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt
    39901 gccggaaaac gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca
    39961 gaaatatgac caacagcaaa ggccatcgtc agaacggtca gaccgaacgc cagcgccacg
    40021 ccggcaaaac caatgcctaa ttccgggaag cctgcggcca gtacagcact accacagcca
    40081 ccaaaaacaa gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc
    40141 tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt
    40201 gacgacggaa ataatgcgcg gctattttaa aaacgaaggc gagtcattca ccagataaat
    40261 aaatccagta aatttgattt agggcaacag cgggttgccc catatagtca tttgtctgat
    40321 tgacagtg
//
LOCUS       DAMZII010000029        39810 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZII010000029 DAMZII010000000
VERSION     DAMZII010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.029"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(99..774)
                     /locus_tag="POA77_001496"
                     /pseudo
     CDS             complement(99..774)
                     /locus_tag="POA77_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(919..1527)
                     /locus_tag="POA77_001497"
     CDS             complement(919..1527)
                     /locus_tag="POA77_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDI6364957.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGNNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1711..2028)
                     /gene="metJ"
                     /locus_tag="POA77_001498"
     CDS             complement(1711..2028)
                     /gene="metJ"
                     /locus_tag="POA77_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDI6364958.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2305..3465
                     /gene="metB"
                     /locus_tag="POA77_001499"
     CDS             2305..3465
                     /gene="metB"
                     /locus_tag="POA77_001499"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDI6364959.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3468..5900
                     /gene="metL"
                     /locus_tag="POA77_001500"
     CDS             3468..5900
                     /gene="metL"
                     /locus_tag="POA77_001500"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDI6364960.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            6249..7139
                     /gene="metF"
                     /locus_tag="POA77_001501"
     CDS             6249..7139
                     /gene="metF"
                     /locus_tag="POA77_001501"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDI6364961.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEIYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7468..9648
                     /gene="katG"
                     /locus_tag="POA77_001502"
     CDS             7468..9648
                     /gene="katG"
                     /locus_tag="POA77_001502"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDI6364962.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9742..10647
                     /gene="yijE"
                     /locus_tag="POA77_001503"
     CDS             9742..10647
                     /gene="yijE"
                     /locus_tag="POA77_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDI6364963.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10674..11291)
                     /locus_tag="POA77_001504"
     CDS             complement(10674..11291)
                     /locus_tag="POA77_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDI6364964.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11566..12669)
                     /gene="gldA"
                     /locus_tag="POA77_001505"
     CDS             complement(11566..12669)
                     /gene="gldA"
                     /locus_tag="POA77_001505"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDI6364965.1"
                     /translation="MDHIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFT
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12680..13342)
                     /gene="fsa"
                     /locus_tag="POA77_001506"
     CDS             complement(12680..13342)
                     /gene="fsa"
                     /locus_tag="POA77_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI6364966.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13354..15855)
                     /gene="ptsP"
                     /locus_tag="POA77_001507"
     CDS             complement(13354..15855)
                     /gene="ptsP"
                     /locus_tag="POA77_001507"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6364967.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16164..17243
                     /locus_tag="POA77_001508"
     CDS             16164..17243
                     /locus_tag="POA77_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDI6364968.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17258..17578
                     /locus_tag="POA77_001509"
     CDS             17258..17578
                     /locus_tag="POA77_001509"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI6364969.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17629..19926
                     /locus_tag="POA77_001510"
     CDS             17629..19926
                     /locus_tag="POA77_001510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6364970.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19892..20770
                     /locus_tag="POA77_001511"
     CDS             19892..20770
                     /locus_tag="POA77_001511"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDI6364971.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20772..21113
                     /locus_tag="POA77_001512"
     CDS             20772..21113
                     /locus_tag="POA77_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI6364972.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21100..21951)
                     /locus_tag="POA77_001513"
     CDS             complement(21100..21951)
                     /locus_tag="POA77_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6364973.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22166..23899)
                     /locus_tag="POA77_001514"
     CDS             complement(22166..23899)
                     /locus_tag="POA77_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDI6364974.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24082..26733)
                     /gene="ppc"
                     /locus_tag="POA77_001515"
     CDS             complement(24082..26733)
                     /gene="ppc"
                     /locus_tag="POA77_001515"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDI6364975.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27085..28236)
                     /gene="argE"
                     /locus_tag="POA77_001516"
     CDS             complement(27085..28236)
                     /gene="argE"
                     /locus_tag="POA77_001516"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDI6364976.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28390..29394
                     /gene="argC"
                     /locus_tag="POA77_001517"
     CDS             28390..29394
                     /gene="argC"
                     /locus_tag="POA77_001517"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI6364977.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29402..30178
                     /gene="argB"
                     /locus_tag="POA77_001518"
     CDS             29402..30178
                     /gene="argB"
                     /locus_tag="POA77_001518"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6364978.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPMVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30239..31612
                     /gene="argH"
                     /locus_tag="POA77_001519"
     CDS             30239..31612
                     /gene="argH"
                     /locus_tag="POA77_001519"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6364979.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31879..32796
                     /gene="oxyR"
                     /locus_tag="POA77_001520"
     CDS             31879..32796
                     /gene="oxyR"
                     /locus_tag="POA77_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDI6364980.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32779..34179)
                     /gene="sthA"
                     /locus_tag="POA77_001521"
     CDS             complement(32779..34179)
                     /gene="sthA"
                     /locus_tag="POA77_001521"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDI6364981.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34510..35160
                     /gene="fabR"
                     /locus_tag="POA77_001522"
     CDS             34510..35160
                     /gene="fabR"
                     /locus_tag="POA77_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDI6364982.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35160..35519
                     /locus_tag="POA77_001523"
     CDS             35160..35519
                     /locus_tag="POA77_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDI6364983.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35559..36659)
                     /gene="trmA"
                     /locus_tag="POA77_001524"
     CDS             complement(35559..36659)
                     /gene="trmA"
                     /locus_tag="POA77_001524"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDI6364984.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36773..36963
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37028..38872
                     /gene="btuB"
                     /locus_tag="POA77_001525"
     CDS             37028..38872
                     /gene="btuB"
                     /locus_tag="POA77_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDI6364985.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38817..39674
                     /gene="murI"
                     /locus_tag="POA77_001526"
     CDS             38817..39674
                     /gene="murI"
                     /locus_tag="POA77_001526"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI6364986.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9047 a        10019 c        11012 g         9732 t
ORIGIN      
        1 agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt aatcatccaa ggaaagctta
       61 tcagaagggt gatgactcca attagtagaa catatatttt cgataaaagc accgatgact
      121 ttttcatgaa gttctacaga acgcgagaaa cagatggttc tgcgggccag acgtttgatt
      181 cgggttctca gggtcagatt gttacgctca atgtgctgag taaaaatctt accagtcaga
      241 tgcttctcct tcggtaactt tctagcgtaa ctaccccatt cgtcagtggt caacatgccg
      301 atggtaaaag ggtaagcagc gcccgcaatt cacgacaggt ttcgtcagtc cgggggccaa
      361 aagtgtaggc cagaacacca ccagttttcg tgttgtacac gtaccagagc cagtgctgtc
      421 gcgctttact gccgacaaag ctccattgct gactaagttt gcagatgagt gccacatcag
      481 catgagcgac cggggatgac gttattcttc gtggcgagag ttttttaaag ttcggatgac
      541 tgtattaatg ccgattttca gcgtacgggc agtatcgcga accccggcac cgttgaaagt
      601 catttcagcg atttggtctt taactcccgg tttacggggc tcgtaacagt aactgagctg
      661 aaatacccgg tgacagtcac ggccgcggaa tcgatcatgt ctttttgggt tctgcccgtg
      721 acggtaaacc tgaacggact ggcaacgggg gcaatgaagg ttaactgtga ccatgaaaca
      781 accttaaaag cgagtattat atccttaatt caactaattg gaggcatcac cgtttaccgg
      841 agcgacagct tgcggaaagg tcgtgcaact acaaagcaac aaacagacta cggcgggaat
      901 tatttttttc atcatccatt accacgaaaa cagcggccac tctttggcaa ccgtggtgag
      961 ttgtggcgcg tcgaaaacgg cttgtggtga aactaccgtt tcctcaagcg gaatattatt
     1021 accgtagcgt tctttgagct tcgcctggac cagcgggttg ctgagatcaa actctttcaa
     1081 cttctgctgt tcgcccacgc gcattcccag cgcgttctgg taaaccttcc acaccacttc
     1141 cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcatacggtt taccgagata
     1201 acgttttgcc gtttgcgcca gtttttgctg ttgttcaaca ctcagtccgc cttcaacgcg
     1261 gcgaacgacg tatttgccct tttcaccatg ggcgatccac tgcttgagcg gggtgtattt
     1321 caccgggccg accgcttcaa aaacgtaggg ctttttgttg cgtatcacca gcataccggt
     1381 gtggctgtaa tcggaatggg ttgccagttg gatcgcttta ctttgcgatg agcgagagat
     1441 ttgaaagatg atgtcgccag tttgcggctg ccaggcaaag gcaggtacag aaaccagcag
     1501 gctgaggatc agcagcctgt ttttcatagt taaacgtcca tgtataaaaa gcggtgggtc
     1561 gcagacaacg tgctcgttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa
     1621 gttcgtgcaa attcaataaa ttgcaatatg acgtaggcct gataagcgta gcgcatcagg
     1681 cggttccact ccgcgccgct cttttccgct ttagtattcc cacgtctccg ggttaatccc
     1741 catctcacgc atgatctctt ttgccgcttc cgggatttcg tcgctgcgct ctttacgcag
     1801 atcggcatca tccggcaaag gttgcccggt aaaggcatgc agaaacgctt cgcacagcag
     1861 ctcgctgttg gtagcgtgac gcaggttgtt cacctgacga cgcgtgcgtt catcggtgag
     1921 gatttttaac accttaagag gaatggaaac cgtaatcttt ttgacttgtt cactcttctt
     1981 gccgtgctca gcgtatgggc tgatatattc gccgctccat tcagccatga gatacttaat
     2041 cctcttcgtc aataaattga gaccagacca cacaatttat gtgggtactg accgtaaacc
     2101 cgcatagttt accgtacagg cgttaccgtg acatcgtgta atgcacctgt cggcgtgata
     2161 atgcatataa ttttaacggc tatttgggat ttgctcaatc tatacgcaaa gaagtttaga
     2221 tgtccagatg tattgacgtc cattaacaca atgtttactc tggtgcctga catttcaccg
     2281 acaaagccca gggaacttca tcacatgacg cgtaaacagg ccaccatcgc agtgcgtagc
     2341 gggttaaatg acgacgaaca gtatggttgc gttgtcccac cgatccatct ttccagcaca
     2401 tataacttta ccggatttaa tgaaccgcgc gcgcatgatt actcgcgtcg cggcaaccca
     2461 acgcgcgatg tggttcagcg tgcgctggca gaactggaag gcggtgctgg tgcagtactt
     2521 actaataccg gcatgtccgc gattcacctg gtaacgaccg tctttttgaa acctggcgat
     2581 ctgctggttg cgccgcacga ctgctacggc ggtagctatc gcctgttcga cagcctggcg
     2641 aaacgcggtt gctatcgcgt gttgtttgtt gatcaaggcg atgaacaggc attacgggca
     2701 gcgctggcag ataaacccaa actggtactg gtagaaagcc caagtaatcc attgttacgc
     2761 gtcgtggata ttgcgaaaat ctgccatctg gcaagggaag tcggggcggt gagcgtggtg
     2821 gataacacct tcttaagccc ggcattacaa aatccgctgg cattaggtgc cgatctggtg
     2881 ttgcattcat gcacgaaata tctgaacggt cactcagacg tggtggccgg cgtggtgatt
     2941 gctaaagacc cggacgttgt cactgaactt gcctggtggg caaacaatat tggcgtgacg
     3001 ggcggcgcgt ttgacagcta tctgctgcta cgcgggttgc gaacgctggt gccgcgtatg
     3061 gagctggcgc agcgcaacgc gcaggcgatt gtgaaatatc tgcaaaccca gccgttggtg
     3121 aaaaaactgt atcacccgtc gctgccggaa aatcaggggc atgaaattgc cgcgcgtcag
     3181 caaaaaggct ttggcgcaat gttgagtttt gaactggatg gcgatgagca gacgctgcgt
     3241 cgtttcctgg gcgggctgtc gttgtttacg ctggcggaat cattaggggg agtggaaagt
     3301 ttaatctctc acgccgcaac catgacacat gcaggcatgg caccagaagc gcgtgctgcc
     3361 gccgggattt ccgagacgct gctgcgtatc tccaccggta ttgaagatgg cgaagattta
     3421 attgccgacc tggaaaatgg cttccgggct gcaaacaagg ggtaaaaatg agtgtgattg
     3481 cgcaggcagg ggcgaaaggt cgtcagctgc ataaatttgg tggcagtagt ctggctgatg
     3541 tgaagtgtta tttgcgtgtc gcgggcatta tggcggagta ctctcagcct gacgatatga
     3601 tggtggtttc cgccgccggt agcactacta accagttgat taactggttg aaactaagcc
     3661 agaccgatcg tctctctgcg catcaggttc aacaaacgct gcgtcgctat cagtgcgatc
     3721 tgattagcgg tctgctaccc gctgaagaag ccgatagcct cattagcgct tttgtcagcg
     3781 accttgagcg cctggcggcg ctgctcgaca gcggtattaa cgacgcagtg tatgcggaag
     3841 tggtggggca cggtgaagtg tggtcggcac gcctgatgtc tgcggtactt aatcaacaag
     3901 ggctgccagc ggcctggctt gatgcccgcg agtttttacg cgctgaacgc gccgcacaac
     3961 cgcaggttga tgaagggctt tcttacccgt tgctgcaaca gctgctggtg caacatccgg
     4021 gcaaacgtct ggtggtgacc ggatttatca gccgcaacaa cgccggtgaa acggtgctgc
     4081 tggggcgtaa cggttccgac tattccgcga cacaaatcgg tgcgctggcg ggtgtttctc
     4141 gcgtaaccat ctggagcgac gtcgccgggg tatacagtgc cgacccgcgt aaagtgaaag
     4201 atgcctgcct gctgccgttg ctgcgtctgg atgaggccag cgaactggcg cgcctggcgg
     4261 ctcccgttct tcacgcccgt actttacagc cggtttctgg cagcgaaatc gacctgcaac
     4321 tgcgctgtag ctacacgccg gatcaaggtt ccacgcgcat tgaacgcgtg ctggcctccg
     4381 gtactggtgc gcgtattgtc accagccacg atgatgtctg tttgattgag tttcaggtgc
     4441 ccgccagtca ggatttcaaa ctggcgcata aagagatcga ccaaatcctg aaacgcgcgc
     4501 aggtacgccc gctggcggtt ggcgtacata acgatcgcca gttgctgcaa ttttgctaca
     4561 cctcagaagt ggccgacagt gcgctgaaaa tcctcgacga agcgggatta cctggcgaac
     4621 tgcgcctgcg tcaggggctg gcgctggtgg cgatggtcgg tgcaggcgtc acccgtaacc
     4681 cgctgcattg ccaccgcttc tggcagcaac tgaaaggcca gccggtcgaa tttacctggc
     4741 agtccgatga cggcatcagc ctggtggcag tactgcgcac cggcccgacc gaaagcctga
     4801 ttcaggggct gcatcagtcc gtcttccgcg cagaaaaacg catcggcctg gtattgttcg
     4861 gtaagggcaa tatcggttcc cgttggctgg aactgttcgc ccgtgagcag agcacgcttt
     4921 cggcacgtac cggctttgag tttgtgctgg caggtgtggt ggacagccgc cgcagcctgt
     4981 tgagctatga cgggctggac gccagccgcg cgttagcctt cttcaacgat gaagcggttg
     5041 agcaggatga agagtcgttg ttcctgtgga tgcgcgccca tccgtatgat gatttagtgg
     5101 tgctggacgt taccgccagc cagcagcttg ctgatcagta tcttgatttc gccagccacg
     5161 gtttccacgt tatcagcgcc aacaaactgg cgggagccag cgacagcaat aaatatcgcc
     5221 agatccacga cgccttcgaa aaaaccgggc gtcactggct gtacaatgcc accgtcggtg
     5281 cgggcttgcc gatcaaccac accgtgcgcg atctgatcga cagcggcgat actattttgt
     5341 cgatcagcgg gatcttctcc ggcacgctct cctggctgtt cctgcaattc gacggtagcg
     5401 tgccgtttac cgagctggtg gatcaggcgt ggcagcaggg cttaaccgag cctgatccgc
     5461 gtgacgacct ctccggcaaa gacgtaatgc gcaagctggt aattctggcg cgtgaagcgg
     5521 gttacaacat tgaaccggac caggtacgtg tggaatcgct ggtgcctgct cattgcgaag
     5581 gcggcagcat cgaccatttc tttgaaaatg gcgatgaact taacgagcag atggtgcaac
     5641 ggctggaagc ggcccgcgaa atggggctgg tgctgcgcta cgtggcgcgt ttcgatgcca
     5701 acggcaaagc gcgtgtgggc gtggaggcgg tgcgtgaaga ccatccgctg gcatcactgc
     5761 tgccgtgcga taacgtcttt gccatcgaaa gccgctggta tcgcgacaac ccgctggtga
     5821 tccgtgggcc tggcgcgggg cgcgacgtca ccgccggggc gattcagtcg gatatcaacc
     5881 ggctggcaca gttgttgtaa tttcagaaat ttaataatgc ccggtactca tgttttcggg
     5941 tttatggttt ctaatgaaat atattgaatt atcataggat taggccggat taagcgttta
     6001 cgacgaatcc ggcaagaagc aataagtaca tggttagttt atatttgcag tccggtttgc
     6061 tttgcatacc ggattttctt tttcttacca tcctgaagtt ttttcatctt ccctgatttt
     6121 tcctcaccat cattggtcat ttttcggttg acgcccttcg gcttttcctt catctttaca
     6181 tctggacgtc taaacggata gatgtgcaca acacaacata taactacaag cgattgatga
     6241 ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag agcctggcag
     6301 aagtccaggg gcaaattaac gtttcgttcg agtttttccc gccgcgtacc agtgaaatgg
     6361 agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag tttgtatcgg
     6421 tgacctatgg cgcgaactcc ggcgagcgcg accgtacgca cagcattatt aaaggcatta
     6481 aagatcgcac tggtctggaa gcggcaccgc atcttacttg cattgatgcg acgcccgacg
     6541 agctgcgcac cattgcacgc gactactgga ataacggtat tcgtcatatc gtggcgctgc
     6601 gtggcgatct gccgccggga agtggtaagc cggaaatata tgcttctgac ctggtgacgc
     6661 tgttaaaaga agtggcagat ttcgatatct ccgtggcggc gtatccggag gttcacccgg
     6721 aagcaaaaag cgctcaggcg gatttgctca acctgaaacg caaagtcgat gccggagcca
     6781 accgcgcgat tactcagttc ttctttgatg tcgaaagcta cctgcgtttt cgtgaccgct
     6841 gcgtatcggc gggtattgat gtggaaatta ttccgggaat tttgccggtc tcgaacttta
     6901 aacaagcgaa gaaatttgcc gatatgacca acgtgcgtat tccggcgtgg atggcacaaa
     6961 tgtttgacgg gctggacgac gatgccgaaa cccgcaaact ggtgggagcg aatattgcca
     7021 tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc tatacgctta
     7081 accgcgctga aatgagttac gcgatttgcc atacgctggg tgttcgacct ggtttataaa
     7141 tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag cacaaaatgc
     7201 tgcctcgaaa tgagggcggg aaaataaggt tgtcagcctt gttttctccc tcattacttg
     7261 aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg acataatcaa
     7321 aaaagcttaa ttacgatcaa tttgacctac atctctttaa ccaacaatat gtaagatctc
     7381 aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt gtatcgtaac
     7441 ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca taacacgaca
     7501 gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcggg ggcgggcaca
     7561 accactcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca acattctaat
     7621 cgttctaacc cgctgggtga ggactttgac taccgcaaag aattcagcaa attagattac
     7681 tacggcctga aaaaagatct gaaagccctg ctgacagaat ctcagccgtg gtggcctgcc
     7741 gactggggca gctacgccgg tctgtttatt cgtatggcct ggcacggcgc ggggacttac
     7801 cgttcaatcg atggacgcgg cggcgcgggt cgtggtcagc aacgttttgc accgctgaac
     7861 tcctggccgg ataacgtaag cctcgataaa gcgcgtcgcc tgttgtggcc aatcaaacag
     7921 aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcgggtaa cgtggcgctg
     7981 gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt ctgggaaccg
     8041 gatctggatg ttaactgggg tgatgaaaaa gcctggctga cccaccgtca tccggaagcg
     8101 ctggcgaaag caccgctggg tgcaaccgag atgggcctga tttacgttaa cccggaaggc
     8161 ccggatcaca gcggcgaacc gctttctgcg gcagcagcta tccgtgcgac cttcggcaac
     8221 atgggcatga acgacgaaga aaccgtggcg cttattgcgg gtggtcatac gctgggtaaa
     8281 acccacggtg ccggtccgac atcaaatgta ggtcctgatc cagaagctgc accgattgaa
     8341 gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga tgccattacc
     8401 tctggtctgg aagtagtctg gacccagacg ccgacccagt ggagcaacta tttcttcgag
     8461 aacctgttca agtatgagtg ggtacagacc cgcagcccgg ctggcgcaat ccagttcgaa
     8521 gcggtagacg caccggaaat tatcccggat ccgtttgatc cgtcgaagaa acgtaaaccg
     8581 acgatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa gatctctcgt
     8641 cgtttcctca acgatccgca ggcgttcaac gaagcctttg cccgtgcctg gttcaaactg
     8701 acgcacaggg atatggggcc gaaatctcgc tacatcgggc cggaagtgcc gaaagaagat
     8761 ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca ggacattatc
     8821 gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagctggt atcggtggcc
     8881 tgggcttctg cttctacctt ccgtggtggc gacaaacgcg gtggtgccaa cggtgcgcgt
     8941 ctggcattaa tgccgcagcg cgactgggat gtgaacgccg cagccgttcg tgctctgcct
     9001 gttctggaga aaatccagaa agagtctggt aaagcctcgc tggcggatat catcgtgctg
     9061 gctggtgtgg ttggtgttga gaaagccgca agcgccgcag gtttgagcat tcatgtaccg
     9121 tttgcgccgg gtcgcgttga tgcgcgtcag gatcagactg acattgagat gtttgagctg
     9181 ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggacgt ttccaccacc
     9241 gagtcactgc tgatcgacaa agcacagcaa ctgacgctga ccgcgccgga aatgactgcg
     9301 ctggtgggcg ggatgcgtgt actgggtgcc aacttcgatg gcagcaaaaa cggcgtcttc
     9361 actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga tatgcgttac
     9421 gagtggaaag cgactgacga atcgaaagag ctgttcgaag gtcgtgaccg tgaaaccggc
     9481 gaagtgaaat acacagccag ccgtgcggat ctggtatttg gttctaactc tgtcctgcgt
     9541 gcggtagcgg aagtttacgc cagcagcgat gcccacgaga agtttgttaa agacttcgtg
     9601 gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc tgaccttctt
     9661 cagcgactgt ctttcaggca gtcgctgaag tttctttacc ggcgtatagt gtccacagga
     9721 aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact ggcaatcagt
     9781 ggcctggttg tgctcacact tatctggagt tatagctgga ttttcatgaa gcaagtcacc
     9841 agctacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc tctcgtttta
     9901 ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga caccgtttaa atacacctta
     9961 gccattgccc tgttacaaac ctgcgggatg gttggcctgg cgcagtgggc gctggtcagc
    10021 ggcggagcgg ggaaggtggc gatcctaagc tataccatgc cgttctgggt ggtgattttc
    10081 gccgcgttgt ttctcggtga acgcctgcga cgtgggcaat atttcgcgat actgattgcc
    10141 gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc aatgaaaagt
    10201 gccatgctgg caatcctctc cggcgtcagt tggggggcga gcgcgattgt tgctaaacgc
    10261 ctatacaccc gtcatccgcg cgtggattta ttatcgttaa catcctggca gatgctctat
    10321 gcggcgctgg tgatgagtgt ggtcgcttta ctggtgccgc aacgtgaaat tgactggcag
    10381 cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacggcgct ggcgtggagc
    10441 ttatggttgt ttgtattgaa aaacttgcct gccagtattg ccagcttaag cacgctggcc
    10501 gttcccgttt gcggcgtact cttttcctgg tggctgctcg gtgagaatcc gggggccgtt
    10561 gaaggtagcg gtattgtgct gattgtgctg gcactggcgc tggtgagccg taagaaaaaa
    10621 gaagctgtca gtgtaaaaag catctgaatt ttttcttcat gtggggcgat cgcttattta
    10681 acaaaataac gataatgccc caccatccgc cagctaaaca gcacatcttc ttcctgcgcg
    10741 cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc atctgacacc
    10801 accccaatat gtgccagtcc gttatccagt cgccaggaga caatatcgcc cgcttgatag
    10861 tcactggggt tcttgctgat ggggcgtgtt ttatcgtggc gggtaaacca ggtttccaga
    10921 ttaggcaccc gacggtgatc gatgttgctg tccgggcgct ttaactgcca tttttgcggg
    10981 tactcggcaa aattcttcgc catatcttcg tgcaccagtt tctgcaaatc gaccttctgg
    11041 ctgcgcaatg cacggatcac cacatcggaa cataccccgc gttcttgcgg gacatcaccg
    11101 ccaggatagg taagctgcat atacgccgga tcgtaaaata gcgtgctgcc gatttgctgt
    11161 ctggcaccgt ctgcgatggc aaggttggta ttggcctgga tttgcaccac ggttggtgga
    11221 acggcgggag attttaagga gtggctggta aatgccgtta gcaggctgag cagcgccagt
    11281 gaagctttca tctggttcgt ccgtgaaaat aagagtcaca gattcgacct tccgggctaa
    11341 cgattgttct gtggagaaaa tgtccagaag cggataaaaa actcctgaac aacgtgggag
    11401 tttcagatta ctggcacaga gtgccggatg cggagtgaac gccttatccg gcctacaaaa
    11461 gcacgcatat tcaatatatt gcagagattt cgtaggcttg ataaacgtag cgcatcaggc
    11521 aattttgcgt tcaaactccc ggacaagccg ggagtttgga gtaggttatt cccactcttg
    11581 caggaaacgc tgaccgtact ggtcggctac cagcagagcg gcgtaaacct gatctggcgt
    11641 cgcgccgcca ggcatgttgt gaatggtttc accttctgca catgccgctt ctgccacaat
    11701 tcgcattttc gccgggacat cttctttaat atccagttga gcgagagtta ttggcaaacc
    11761 taccgcatgg ctaagtgcag ctacggtttc gatttcctca accggtgcgt tttccagaac
    11821 cagctgcgtc agcgtaccaa atgccacttt ttcaccgtga taatagtgat gcgcgtccgg
    11881 gatagcggtc aggccgttat gcaccgcgtg agccgccgcc agcccgccgc tttcaaaacc
    11941 aacgccgctc aaataggtgt tcgcttcaat cacgcgctcc agcgccgggg ttactacatg
    12001 ctgttcggcg gcaagcatcg ctttttcgcc ttcttccagc agggtgttgt agcacagttc
    12061 agccagtgcc agcgccgcct gggtgcactt gccgcccgcc atggtggtcg cgccgctacg
    12121 agagcaagca cgcgcttcaa accaggttgc cagcgcatcg ccgatacccg ctgctaacag
    12181 acgtgcaggt gcgcccgcga cgattttggt gtcgacaatg accatattcg ggttatttgg
    12241 caacagcaga tagcggtcaa actcaccctc atcggtgtag ataacagaca atgcgctgca
    12301 cggtgcatcg gtagaggcga tagtcggtgc gatcgctacc ggaacaccca tgaaatgtgc
    12361 cagtgctttg gcagtatcga gggtttttcc gccaccgata ccgagaattg cgccacactg
    12421 cgcagtctcc gcgatgccac gcagacggtc gatctcattt tgcgaacatt caccgccaaa
    12481 cggcgcaatt tctactacca gtccagcatc tttaaagctt ttctcgacag tggattgagt
    12541 aaaacctaaa acaaatttgt cacccaccac taaccagcgt tctgccagcg gcttcaggta
    12601 ttcgcccaga cgattaatca catcagcgcc ctggatgtat ttacccggtg attgaataat
    12661 gtggtccata attgctcctt tagagatgag tagtgccaaa tgcggcattc cagtcgtgtt
    12721 cgaacttctc tatagctgac tctaccgcag gggtgttgag catttgttgc gctacatcta
    12781 agggcagggt gatggattca catcctgcca gtaaacagtc cagcgcctga cgcggcgttt
    12841 taaagctggc cgccagcacc atgctttctg gtgcgtgcat ttctaacagc gcttgcagct
    12901 cctgaaccgt acgaatgccg tccccgccct gggcatctac gcggttaaca tacggagcaa
    12961 cgtattttgc ccctgccagc gcggccagta acccttgtgc agcactgtac actgcggtgc
    13021 caagcgtagt aataccctct tttttcagca tcttaatggc ggccagacct tcggaagtca
    13081 ccgggatttt caccacaatg cccggaatgg cgtcgcgcag gcgcttcgct tcttccacca
    13141 taccctgcgc gtcacgactc atagtctgag caaacagaat gccctcatca ccaatcgctt
    13201 tttgcagacg cggcagaact tcccatatgg actccttgct ggcagcgata atgctcgggt
    13261 tagttgtcac cccggcaatg gggaatatgc gtgccagacg ttcgacttct gcgacgttag
    13321 cggtgtccag atacagttcc atgatgtttc cttttacagt tccagttcat gttgcagcag
    13381 gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg ttacggaatt cttcgtgcat
    13441 gatgcggcga gcgaggcgcg agaaaatgcg catgtgctga tcgcccgcag cgtgtttgtt
    13501 cagggttaac ataatgatga attgcgcttc atcatcgccc cagcgcaccg gcgcttgcag
    13561 acgcgccacg ctgatggtgg attgctcaat gtgttctgat ttgctgtgtg gaatggcaaa
    13621 actaaagccc agaccggtag agaaaacggc ctcgcgcgcc cataagtcgg cttccagttt
    13681 acgcggatag cggcagcggc ccgccagcag caggttatcg gtcatgcctt tgagcacttc
    13741 ttctttgctg cgccagtcac tttccagcgt gatgcactcg gcggtgacca gcggtgcgtc
    13801 ctgttgggtc atgcggaatt gcgccagcag gtgttccact tccagcgaag tacggcaggc
    13861 cattgcttgg ttgagcaact tgcggcactc acggctatca agttgcgcca tccgcgcttt
    13921 cgccgccgga attgatggtg cgctcatgct gagttcatcc agccctaagc cgaccagcaa
    13981 cggcagcacg gaacctttcg ctcccagctc accgcacaga ccaatccatt tgccctggcg
    14041 atgcaccgcc tgcacggcgt aatcgagcgc ccgcaagaat gccggattca ggctgttgta
    14101 gtgacgagta accttagcgt tatcgcgatc cactgccagc agatattgcg tcaggtcatt
    14161 actaccaata ctaaagaaat cagtctcttc gcagcattga tcgatgatga acatcaccga
    14221 cggtacttcc agcataatgc caagctggat cttctcatca aacggaatgt gttcgttacg
    14281 cagttgctgt ttggcttccg ccagtttttc tttcacccat aagatctctt ccattgagga
    14341 gatcatcggg atcatgattt tcaggctgcc gtgagcggag gcgcgcagga tcgaccgtag
    14401 ttgtgtggtg aacagcgagg cgtattcttc ataaatacgc acagcgcgat aaccgaggaa
    14461 cgggttagct tctgccggaa tgttcagata atcaacgggt ttgtcgccgc caatgtccat
    14521 agtgcgcaca ataatgctgc gtccgttggc agactccagc gcctgacaaa aaatgttgta
    14581 cagctcgctt tcgcccggtg cactggtgcg atccatatag agcatttcag tgcggaacaa
    14641 accaacgcct tccgcaccat tgccgaatgc ggcctgcgct tccacggagt gagcgatgtt
    14701 agcggcaatt tcaatgcgga taccgtcagc ggtacgggct tgttgggtca gccagacacg
    14761 ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc tgataataac gagctacggc
    14821 ttctcctggc tcaaccacaa tcgccccagc gttgccgtcg atataaatcg tttgatgctg
    14881 ccacggagta agggcatcaa tatctacacc aaccagtgtc ggaatgttga acgaacgggc
    14941 aagaattacg gtatgtgagg tggtgccgcc acttttcagt aacagacctt tgaggtgatt
    15001 tttatccagt tcgaggaact ggctgggagt cagttcatca gccatacaaa tggcgggctg
    15061 cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag atttgctgga gtaactggaa
    15121 gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag ctgctgctgg aacgggcgaa
    15181 ctcttcgcaa aagtgattcg cactggtgac aatcgcttcg gcgcagctta atccggcgct
    15241 gacgcctgcc agtaaatgtt cgcgcaggga agtatcgcca gccagggatc ggtgcgcttc
    15301 cagaatcgcg ctggtggcac cgtcgctatc cagcagacga aactcaatgt ttttcagtac
    15361 cagcgtcagg ccgttttcca gtgcggattg ctcggcgtca acgcttttgg ctgcgggaag
    15421 attacccagc gcattgagat ctaaagaaga gatcggcgtc agaatgccgc ctgcactacc
    15481 gctgcacacg gtgcgggcgc ggataatttg cggattcaga ttggtcagtg aaaccggcag
    15541 tggttccagt tcgtcagatt taacttccgc cagcggcgca tcgcagtggg ggaattcatc
    15601 gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg tcggccccgg aaatcagtaa
    15661 ctggcagtta tcgcccgcca gcgtatcggt gccaatcagc gcaagggcgc ttttggcgtt
    15721 gcccttgcgg tcagtgcgca ggttatgcca ctcaatttgt gatgaaaaag tattacacag
    15781 cgtttcaacg tggcttgccg gacgtgcatg tacgccgtta ggtagctcac aaataaattc
    15841 cacaatcagg gccattgcct ctctcccggt acggttttct gtttacagga taagagcgca
    15901 cggcaacggc ctgccatgtg acaaatctgc caaaagctgg acaaatgtaa tgtaactgtc
    15961 aatttgcgac gcgtctcaca agacgctgtt ttgcggcatg cttccggttt atcgcaagtt
    16021 atgaggcgga tcgcattttt gtactgatat tacaaaagtc cagtaaatgg cctttttatc
    16081 cactgtttgc tccgcctgcg attgcctatt gtttcctcca tcaaaaatat ggacatgggc
    16141 tttcgcaagc ccgggagcag cttatgaatg agttggtgca gatcctgaaa aatacccgtc
    16201 agcatttaat gacgggcgtt tcacacatga ttcccttcgt ggtatcgggc ggtattttgc
    16261 tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc ggatgccgta gccgatccaa
    16321 atctgaaaaa actgtttgat atcggcgttg cgggcttgac gctgatggtg cctttcctcg
    16381 cagcgtacat cggttattcc attgcagagc gttctgcgct ggctccgtgc gctatcggtg
    16441 cctgggttgg taacagcttt ggtgcgggct tctttggtgc actgatcgcc gggattatcg
    16501 gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca taaagttctg cgctcggtga
    16561 tgcctatctt cattattcct atcgtcggca cactgattac cgcaggcatc atgatgtggg
    16621 gcttgggcga gcctgtaggg gcgttgacca acagcctgac tcagtggctt caggggatgc
    16681 agcagggcag cattgttatg ctggcggtga tcatgggtct gatgctggcg ttcgatatgg
    16741 gcggtccggt taacaaagtg gcctatgcct tcatgctgat ttgcgttgct cagggtgttt
    16801 ataccgtggt ggccatcgct gccgtgggta tttgtatccc gccgctgggg atggggctgg
    16861 cgacgctgat tggtcgtaaa aatttctccg cagaagagcg cgaaaccggc aaagcggcac
    16921 tggtgatggg ctgtgttggc gttactgaag gggcgattcc tttcgccgct gccgatccgc
    16981 tgcgtgtcat tccttccatc atggtcggtt cagtttgtgg tgcagtaact gcggcgctgg
    17041 tcggtgcgca gtgctatgca ggctggggtg gtctgattgt gctgccggtg gttgaaggca
    17101 agctgggtta tatcgcagca gtggctgtcg gagcagtggt gacggctgtt tgtgtgaacg
    17161 tgctgaaaag tctggcgcgt aaaaatgggt cttcgactga tgaaaaagaa gacgacctgg
    17221 atttggattt tgaaattaat taattgagga acgagccatg acgaaaatta ttgcagtaac
    17281 cgcatgtcct tcaggtgttg cccataccta tatggcagca gaggcgctgg aaagtgccgc
    17341 gaaagcgaaa ggctgggaag tgaaagtaga aacccagggg tcaattggtc tggaaaatga
    17401 actgactgcg gaagatgtgg cgagcgctga tatggtgatt ctgaccaaag atatcggcat
    17461 caagtttgaa gagcgttttg ctggcaagac catcgtgcgc gtcaacatca gcgatgcagt
    17521 aaaacgtgct gacgccatta tgagcaaaat tgaagctcac ctggcgcaaa ccgcttaagt
    17581 tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg agttcctcat gacgaatcgt
    17641 atctctcgcc tcaaaactgc actgtttgcc aatacccgtg aaatctcgct ggagcgggcg
    17701 ctgctttata ccgccagcca tcggcaaacc gaaggcgaac cggtgatatt gcgccgggcg
    17761 aaagcaacag cgtatatcct tgaacatgtt gaaatttcga ttcgtgatga agaactgatt
    17821 gccggtaacc gcaccgtaaa accgcgcgct gggattatgt cgccggaaat ggacccttac
    17881 tggctgctga aagagctgga tcaattcccg acgcgtccgc aggaccgctt tgctatcagt
    17941 gaagaagata aacgtatcta ccgtgaagag ttgttcccgt actgggaaaa acgttcgatg
    18001 aaagatttca tcaacgggca gatgacagat gaagtaaaag ccgcgaccag cacgcagatt
    18061 ttcagcatca accagacaga taaaggccag gggcacatta ttattgatta cccacgcctg
    18121 ctgaatcacg ggctggggga gctggtggcg cagatgcagc aacattgtca gcaacagccg
    18181 gagaatcact tttatcaggc cgcgctgtta ctgctggaag cctcgcagaa acacattctg
    18241 cgttacgctg aactggcgga aacaatggcg gcaaactgca cagatgccca gcgtcgcgaa
    18301 gaactgctga ctattgcgga gatctcccgc cataacgccg aacataagcc gcagacgttc
    18361 tggcaggcgt gccagttatt ctggtacatg aacatcattc tgcaatacga atccaacgcc
    18421 agttcgctat cgttggggcg cttcgaccag tatatgttgc cgttctatca ggcatcttta
    18481 acccagggcg aagatccggc gttcctgaaa gaactactcg aatctttatg ggtgaaatgc
    18541 aacgacatcg tgctgttgcg ctccaccagc agcgcgcgtt atttcgcagg tttcccgacc
    18601 ggctataccg cactgctcgg cgggttaacc gagaacggac gtagcgcggt gaacgtgctt
    18661 tcgttccttt gccttgacgc ctatcaaagc gtgcaattac cgcaaccgaa cctcggcgtg
    18721 cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa ccgccgaaac cattcgcctc
    18781 ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg taccagcgtt cctcaaccgt
    18841 ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg gctgtgtgga attatctatt
    18901 cccggcagaa cctacggctt gcatgacatc gcgatgttta atctgctgaa agtgatggaa
    18961 atctgcctgc atgaaaatga aggcaatgct gcgctgactt atgaaggttt actggagcag
    19021 atccgcgcca agatcagcca ctacatcacc ctgatggttg agggcagcaa tatttgtgat
    19081 atcggccatc gcgactgggc acctgtaccg ctgctctcgt cttttatcag cgattgtctg
    19141 gaaaaaggtc gcgatattac cgatggcggc gcgcgttata acttctccgg cgtacagggg
    19201 atcggtatcg ccaacctgag cgattctctc catgcgttga aagggatggt ttttgagcaa
    19261 cagcgtttaa gttttgacga attgctgtcg gtattaaaag ccaactttgc aacgccagaa
    19321 ggcgaaaaag tccgtgctcg cttaattaac cgcttcgaga aatacggtaa cgatatcgac
    19381 gaggtggata acatcagcgc cgaactgtta cgccactact gcaaagaagt ggaaaaatac
    19441 cagaacccgc gcggtggtta cttcacgccg ggatcgtata ccgtttctgc tcacgtcccg
    19501 ttgggatcgg tggttggcgc gacgccagac ggtcgttttg ccggggaaca actggcagac
    19561 ggcggcttgt cacctatgct gggccaggat gcacaagggc caacagcggt actgaagtca
    19621 gtcagtaagc tcgataatac gctgctgtct aacggtacgt tgctgaacgt gaaattcact
    19681 ccggcgaccc tggaaggtga agcgggatta cgcaaactgg ccgacttctt acgggcgttt
    19741 acccagctta agttgcagca tattcagttt aacgtggtga acgccgacac gttgcgggaa
    19801 gcgcaacagc gcccacaaga ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc
    19861 ttctttgtcg aactgtcgaa ggagatccag gatgacatca tccgccggac agcgcatcag
    19921 ctgtaacgtt gtggaaacgc gccgcaatga tgtggcgcgc attttcaaca ttcagcgtta
    19981 ttcactgaat gacggcgagg gcattcgtac ggtggtcttt tttaaaggct gtccgcatct
    20041 ttgcccatgg tgtgctaatc cggagtcgat ctccggcaaa atccagacgg tacgcagaga
    20101 ggcgaaatgt ctgcactgtg cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc
    20161 gtttgaacgg attggtcgcg atatcagcct tgacgcgctg gaacgggaag tgatgaaaga
    20221 tgacattttc tttcgcacgt ccggcggcgg cgtcacgctt tctggcggcg aagtgttaat
    20281 gcaggcggag tttgctaccc gttttttaca gcggctgcgg ttgtggggcg tgtcatgcgc
    20341 cattgaaact gccggagacg cgccagccag caagctgtta ccactggcga aattgtgcga
    20401 tgaagtgctg ttcgatttaa aaattatgga cgcgactcag gcgcgggatg tggtgaagat
    20461 gaacctgcca cgcgtgctgg agaatctgcg tttgctggtg agtgagggtg tcaacgtgat
    20521 cccgcgttta ccattgatcc ctggtttcac gctcagccgg gagaatatgc agcaggcgct
    20581 ggatgtactg atcccgctga aaatcaggca gatccatctg ttaccgtttc atcagtacgg
    20641 cgaaccgaaa taccgcctgc tggggaaaac atggtcgatg aaagaggtgc ctgcgccgtc
    20701 gtcagccgat gtgacaacga tgcgcgaaat ggcagaacgg gccggatttc aggttaccgt
    20761 gggaggttaa aatggcatac ctggtggcag taaccgcctg cgtaagcggc gtggcgcata
    20821 cttatatggc ggcggaacgg ctggaaaagt tgtgccagtt agagaagtgg ggcgtcagca
    20881 ttgaaactca gggcgcgctg ggaacggaga atcgtttagc ggacgaggat atccgtcggg
    20941 cggatgttgc tctgttgatt acggatatcg agcttgccgg tgccgagcga tttgaacatt
    21001 gccgctatgt gcaatgcagc atctacgcat tcctgcgtga gccgcagcgg gtaatgagcg
    21061 cggtgcgcaa agtgctttct gcgccgcagc aaacccatct tattctggag tagtcggttt
    21121 ttctgtcagt tggctgtggt actgccgacg atattccgac ggcgagcgtt cggtgttttt
    21181 acgaaacaga cggcagaagt agttgctgtc gacaaagccg caggcgtgcg ccacttcttt
    21241 taccttcagg tcatagcctt tcaataacgt cttagcgtgc tccagtcgcg tgtgattcag
    21301 gtattcgtta aaaccaatgg cccccgtttt ttgaaacagg tgcgagaggt aatttggcga
    21361 aatatagaat gcctgtgcga cagattcgcg ggtaagcgcg gaggcatagc gttcgtcgat
    21421 ataatcgcga atagcttcga atagagcctg gctgcgcgag gcggtctgga tttggctgcc
    21481 gagcaaatcg cggcagtggc taagcaggct ggcgacgata agccttgccg tttgctgctc
    21541 ctgcggctgc atttgcattt cattgagcgt ttgtagcaga aaagaaccta tgcgtgggcc
    21601 gcgccgggcg acgtgttgct tcgccagatt ttgatattgt ttgccatccc attgcacgac
    21661 gctgaagccg agttgctgtt tgccaaacag cacgctaaag gtagtagcgg gggcttgcca
    21721 ttgtggaaaa ttccagccac cagcgggaac gtacagcaca tcgccgggaa ctaacgttgc
    21781 tccagcgccg gtatcaacaa actcaccttc cagcactatt tccagccgtg gaaaatcgac
    21841 ctgatacgca agatcgggcg ccggaccgtt actgctggca aagtaaatct ggcgcaggga
    21901 cagcgggccg ttgatcaggc gggagagcag atagctgacg tcgtgataca tgttattccc
    21961 ttaggtatgt agacgggaaa acagtaactg gcattattaa aaaaggccac ctgaaaagtg
    22021 tggcctgagg ggagttcgat gtaaacattg cggcagttgt attgccggat gtggcgtaaa
    22081 cgccttattc agtctaccgc cgcgatctgt aggccggata agacgcgtca agcgtcgcat
    22141 ccggcaaata gcgcctggct gataattact gattacctac ctggtcgcca tacggcagag
    22201 tgtcgtaatc gatcagcgcg tttttcttgt acgggttacc aatccagcgg gtagtttctt
    22261 tgaactgcgg attcaccact gaacgggttg gatcgtaacc gtcgtaagat aagcccgcca
    22321 aatctgacca ggtgtggatc agttccgcca ggctgtattt acgatcgaca tcctgtgaga
    22381 aatcacgggg atgagtcgct tgccattttt ccgacgtcca cagcaggaac ggaatggtgt
    22441 acatgtggcg cgtcgggttg tcttcgttac gcccctgagt tttgtgcggc ggcgtgtcgt
    22501 aaacctcttc accgtggtca gagaagtaaa ccaggaatcc gttcgggtct gccgctttaa
    22561 agtctttaat caggctggca accacatgat cgttatacag gttagcgttg tcataatcgt
    22621 tatatgattc cagctcttct gcgcttaatc ctggcggaac atgatcggta ttgccatcaa
    22681 acttgccctg attttccggg tagcggtatt tgtatttgat atgcgtaccc agcagatgaa
    22741 cgatgatcag tttcttcggc gcagggtcat tcagcacatc ctggaacggc ttcagcacgt
    22801 tggtgtcgta ttcacgcgca ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg
    22861 tctggcgcga aaataccgtc agcatggtat tgcgggcggt catcgtctgc tggttggtga
    22921 tccagaaggt tttataaccc gcctgtttca tcatgttcat cagcgacggc tgcgtcagat
    22981 acagatccgg gttcttttca ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt
    23041 acggacgaga cgtaactacg ttattaaaca cggtcagatt cggatcggtt ttatgcagcg
    23101 catccagctc cggcgtggtt tcacgcggat aaccgtacag actcatgcgt ccgcgctggg
    23161 tcgactcgcc aatcaccagc actaaagtgc gcggttcgtt acccgattca tctttgaaat
    23221 tagccagcgg cggcaaggca ttattttcat tcagtagctt tgttagcgag tttagttgct
    23281 gacgatactg ataatagccg gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg
    23341 ccaggttatc caacgttttc tcaaacggct tgtttttgat aaacgtattc atggcgatcg
    23401 gatgcagaat caagccataa agcagggcaa aagagacaat ataacgccac ggctttggaa
    23461 tatagaccgg gcgcagacgt gtccacagca gaactgccat tgccgtatag gccagcgcga
    23521 taagcacaat tttcaggctg aaatactggc ttaaatactc gctggcttcg ttggtgttgg
    23581 tttcgaacat cacaaacaga acgctctgcg agaactcctg cccgtagatg acgtagtagc
    23641 atagcgccgc cagagaggcc gcccatagca ccacgccgat tactgcggca ataattttaa
    23701 tccgcttcgg aaagaggaat accgggatca accacagcga gctgaataac agcgagtcgc
    23761 gaatgccgtt ggtgccacta taaccactga tgtaaataat ggcctgtagc agagtagaga
    23821 aaaaccaaaa gtagagcagt gcccaaccca gggctttcca gctaaaaagg ggtttagcct
    23881 ggacttctgt ggaatgcata gtaagaacct gtcttgaaaa aaatatcgcc gaatgtaacg
    23941 acaattcctt aaggatatct gaaggtatat tcagaatttg aataaaatgc agacagaaat
    24001 atattgaaaa cgagggtgtt agaacagaag tatttcagaa aaccctcgcg caaaagcacg
    24061 agggtttgca gaagaggaag attagccggt attacgcata cctgccgcaa tcccggcaat
    24121 agtgaccatt aacgcttgtt cgacgcgagg atccggttcc tggccttctt tttctgcctg
    24181 gcgggagcgg tgcagtaact cggcctgcaa tacgttcagc gggtcggtgt aaatattccg
    24241 tagctgaata gactctgcaa tccacggcag atcggccatt agatgggaat cgttggcaat
    24301 cgccagcacc actttgatgt cttcttcttg caggttgcgt aactctttac ctaacggcca
    24361 cagtgctttg tctaccaggc gttggtcata gtattccgcc agccacaggt ctgctttggc
    24421 gaagaccatc tccagcatgc cgagacgcgt cgagaagaat ggccaatcgc ggcacatggc
    24481 ttccagctcg ctctgtttgc catcttcgac cactttttgc agcgccgtac ctgcacccag
    24541 ccaggcgggg agcatcaggc ggttttgcgt ccaggcaaaa atccacggta tggcgcgcag
    24601 cgactcgacg ccgccggttg ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc
    24661 caattcttgt tccggcgtag cggagcggaa gtaaggcaca aaatctttgt tttcacgtac
    24721 gtagccacgg tagagatcgc aggagatgac tgacagttca tccataatgc gacgccagct
    24781 ctctttcggc tcaggtggtg gcagcaggtt ggcttccaga atcgccccgg tgtacagcga
    24841 caggctgctg acggtgattt ctggcagacc atatttaaag cggatcatct cgccctgttc
    24901 agtcacgcgc aggccacctt tcaggcttcc tggcggttgt gacagcagcg ccgcatgagc
    24961 aggtgcgccg ccgcgaccaa tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc
    25021 cgctttttcg caggttttga ttaatgcatc ctgtgcctga tattgcgccc aggaagctgc
    25081 catcactccc gcatcttttg ctgagtcgga atagccaatc atcaccatct gtttgccctg
    25141 aatcaggcca cgataccagt caatattgag cagctgggtc atgacatcgt tggcgttgtt
    25201 cagatcatcg agggtttcaa acagcggagc aaccggcatc gcaaacccga tacccgcttc
    25261 tttcagcagc aggtggacag ccagtacgtc ggacggcgtt ttcgccatcg agatcacgta
    25321 ggcggcaatg gagccttgcg gtgcttcggc aatcacctgg caggtatcga gcacttcgcg
    25381 cgtttcggcg cttggttgcc agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg
    25441 gatcaggaac gcctgtttgt cggcctctga ccagctttcg tagtcgccga taccgaggta
    25501 gcgggtcagc tcgcccagcg cttcggtatg acgcgtgctc tcctgacgga tatcaatacg
    25561 gaccagcggt acgccgaaac atttcacgcg gcgcagggtg tcgagcagat cgccgttggc
    25621 gataataccc atgccacacg cctgaagtga ctggtagcaa gcgtagagcg gttcccacag
    25681 ttcttcgttt tgtgtcagca gcccttctgg ttttggcagt tcttcgcctt tcaggcgcgc
    25741 ttccagccat gcctgtgtcg ccatcaggcg agaacgcagg tttttcatca gatagcgata
    25801 cggttctgcg gcaccttctt cgccaaccag cgccagcagt tcaggggtcg cttcaaccat
    25861 cgacagttca gaaaccagca cctgaatatc tttcaggaac aaatcggtgg ctttccagcg
    25921 gctgagtagc aggacgtggc gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc
    25981 gccgcccatc cacgaagtaa aacggaccgg aacaaattcg acgggcagtt tgtagccgag
    26041 gttctcttcc agttgttcgt tcagttcgcg caggtaattt ggtacgcctt gccacaggct
    26101 gttttccact acggcaaagc cccatttggc ttcatctacc gggcttggac gcagcttacg
    26161 gatttcatcg gtatgccatg actgggcgat taactggcgc aggcgacgca tcagctggtt
    26221 gtgttcgtag tcagcgatat ctttgttatc gagctgtttt aagcaggcgt tcacttccac
    26281 cattttgtgg atcagtgtac gacgggtaat ttcggtaggg tgagccgtga ggaccagttc
    26341 cagcgacagc gattccactg cttttttgat ggtgtcttcg ctcagttccg gctggttttt
    26401 cagtttacgc agggtgcggg cgatcacttc cgggttgctg gcagcttcgc ctttcggcga
    26461 aatgctgtgg tattgctcgg cggtgttggc caggttcagg aactgactaa acgcacgcgc
    26521 aacgggcagc agctcgtcgt tcgacaaatt ttgtaaggtg gtgagcaact cctggcggtt
    26581 agcatcattg ccagcgcgtg aagatttcga caacttacgg atagtttcta cgcgttcaag
    26641 aatgtgttct cccaacgcat ccttgatggt ttctcccagc actttgccga gcatactgac
    26701 attactacgc aatgcggaat attgttcgtt catattaccc cagacacccc atcttatcgt
    26761 ttgatagccc tgtatccttc acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg
    26821 tcgtctttta taaagccacg taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca
    26881 aacgaataaa tagcaggaat ttacgtcatt aaattcacga cgctttaaat aagcgtaact
    26941 tatggaaatg ttaaaaaatc gccccaagta acaccaaagg tgtaggtcgg ataagatgcg
    27001 caagtatcgc atccgacatt attgcggcac tggagtttga caacagtgcc ggatgcggcg
    27061 cgagcgcctt atccggccta cgttttaatg ccagcaaaaa tggtgaatta cctgggttat
    27121 cagttcgcgg gtgggcttga taaaccgtgt ttccagatat tcatcaggtt gatgagcctg
    27181 attaattgag ccaggcccca acaccagcgt cgggcataac gtttgaataa acggcgcttc
    27241 ggtacagtaa ttcaccactt cggtttttgc tccgagcaat ttctcaacca cttcaaccag
    27301 ttgatgattc ggtgggcatt catagccagg gatcggcgga tgcagctcgt cgaccgtcag
    27361 acgacccggc cagcgttcgc tcaccggagc caatgcatcg ttgagcaaac cattaagttc
    27421 attgagtgtc atgccaggca gcggacgaat atccatatgc aactcacagc aagcgcaaat
    27481 acggttagaa gcgtcgccac cgtgaatatg cccgaggttg agcgtagggt atggcacggt
    27541 aaacgcttcg tagtgataac gttctttcag gttatcgcgc aattgcaaaa tatgcccgat
    27601 ggcgtcgtgc attagttcga tagcgttaac tccgcgtgct ggatcgctgg agtgccccga
    27661 ctggccctga atacggatgg cgttagagat atgaccttta tgtgcgcgta ccggttgtag
    27721 tgacgtcggt tcgccaatga tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata
    27781 acgcgctccg gccatactgg tttcttcatc agcagtcgcc agaatgtaga gcggtttttt
    27841 cagtttcgtg acgtcgacat cgcgtagcgc atcaaggata aacgcaaaaa agcctttcat
    27901 gtcggcggtg cctaagccgt aaagcttgcc gtcatgctcc gtcagtgtaa acggatcacg
    27961 cgtccagcga ccgtcatcaa atggtaccgt atcggtatgc cccgccagca acaagccgcc
    28021 agccccctgt ccgatacttg ccagcatatt aaatttgttg cgagttcctg gcacaggctg
    28081 cacttccaca ttgaagccta aatctttaaa ccagtccgcc agcagagtga ttaaatctgc
    28141 attgctttga tcgagtgcct cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg
    28201 gtaaatctcg ataaatggcg gtaatttgtt tttcattgtt gacacacctc tggtcatgat
    28261 agtatcaata ttcatgcagt atttatgaat aaaaatacac taacgttgag cgtaataaaa
    28321 cccaccagcc gtaaggtgaa tgttttacgt ttaacctggc aaccagacat aagaaggtga
    28381 atagccccga tgttgaatac gctgattgtg ggtgccagcg gctacgctgg cgcagagcta
    28441 gtgacctatg taaatcgcca tccgcatatg aacataaccg ctttgactgt ttcagcgcaa
    28501 agcaatgatg cgggaaagtt aatctccgat ttgcatccgc agctaaaagg catcgttgat
    28561 ctgccgttgc agccgatgtc ggatatcagc gagtttagcc caggggtgga cgtagtgttt
    28621 ctcgccaccg cccatgaagt tagccacgat ttagcgccgc agtttcttga agcgggctgc
    28681 gtggtgttcg acctttccgg cgcgtttcgt gttaacgacg ccaccttcta tgaaaaatat
    28741 tacggcttta cccatcaata cccggaactg ttggaacagg cagcctacgg tctggcggag
    28801 tggtgcggta ataaattaaa agaagcgaat ttgattgcgg tgccgggctg ttatccgacg
    28861 gcggcacagc tggcgctgaa accgttgatt gatgccgatc ttcttgacct caatcagtgg
    28921 ccggtgatca acgccaccag cggcgtgagc ggtgcagggc gtaaagcggc catttcaaac
    28981 agcttttgtg aagttagcct gcaaccgtat ggcgtcttta ctcatcgcca tcaaccagag
    29041 atcgccacac acctcggtgc tgacgttatc ttcaccccac atctgggcaa tttcccgcgc
    29101 ggcattctcg aaaccattac ctgccgcctg aaatcgggtg tgacccaggc gcaagtcgcg
    29161 caagtgttac agcaggcgta tgcccataaa ccgctggtgc ggctgtatga caaaggcgtt
    29221 ccggcgctga aaaatgtcgt tgggctgcca ttttgcgata tcgggtttgc cgttcagggc
    29281 gagcatctga ttattgtggc gaccgaagac aacttactga aaggcgcggc ggcacaagcg
    29341 gtacagtgcg ccaatattcg tttcggctat gcggaaacgc agtctcttat ttaagggtgc
    29401 aatgatgaat ccattaatta tcaaactggg cggcgtactg ctggatagtg aagaggcgct
    29461 ggaacgtctg tttagcgcac tggtgaatta tcgtgagtca catcagcgtc cgctggtgat
    29521 tgtgcacggc ggcggttgcg tggtggatga gctgatgaaa gggctgaatc tgccggtgaa
    29581 aaagaaaaac ggcctgcggg tgacgcctgc tgatcagata gacattatca ccggagcact
    29641 ggcgggaacg gcaaataaaa ccctgttggc atgggcgaag aaacatcaga ttgcggccgt
    29701 aggtttgttt ctcggtgacg gcgacagcgt caaagtgacc cagcttgatg aagagttagg
    29761 tcatgttgga ctggcgcagc caggttcgcc taagcttatc aactccttgc tggagaacgg
    29821 ttatctgccg atggtcagct ccattggcgt aacagacgaa gggcaactga tgaacgtcaa
    29881 tgccgaccag gcggcaacgg cgctggcggc aacgctgggc gcggatctga ttttgctctc
    29941 cgacgtgagt ggcattctcg acggcaaagg gcaacgtatt gccgaaatga ccgccgcgaa
    30001 agcagaacaa ctgattgagc agggcattat tactgacggc atgatagtga aagtgaacgc
    30061 ggcgctggat gctgcgcgca cgttgggccg tccggtagat atcgcctcct ggcgtcatgc
    30121 ggaacagctt ccggcactgt ttaacggtat gccgatgggt acgcggattt tagcttaagt
    30181 tttgttagcc ggaggcgcag ctttccggca ttgaatttca aaataaggaa acagagttat
    30241 ggcactttgg ggcgggcgtt ttacccaggc agcagatcaa cggttcaaac aattcaacga
    30301 ctcactgcgc tttgattacc gtctggcgga gcaggatatt gttggctctg tggcctggtc
    30361 caaagccctg gtaacggtcg gcgtgttaac cgcagaagag caggcgcaac tggaagaggc
    30421 gctgaacgtg ttgctggaag atgttcgcgc caggccacaa caaatccttg aaagcgacgc
    30481 cgaagatatc catagctggg tggaaggcaa actgatcgac aaagtgggcc agttaggcaa
    30541 aaagctgcat accgggcgta gccgtaatga tcaggtagcg actgacctga aactgtggtg
    30601 caaagatacc gttggcgagt tgctgacggc taaccggcag ctgcaatcgg cgctggtgga
    30661 aaccgcacaa aacaatcagg acgcggtaat gccaggttac actcacctgc aacgcgccca
    30721 gccggtgacg ttcgcgcact ggtgcctggc ctatgttgag atgctggcgc gtgatgaaag
    30781 ccgtttgcag gatgcgctta agcgtctgga tgtcagccca ttaggctgtg gcgcgctggc
    30841 gggaacggcc tatgaaatcg accgtgaaca gttagcaggt tggctgggct ttgcttcggc
    30901 gacccgtaac agtctggaca gcgtttctga ccgtgaccat gtgttggaac tgctttctgc
    30961 tgccgctatc ggcatggtgc atctgtcgcg ttttgctgaa gatctgattt tctttaacac
    31021 cggcgaagcg gggtttgtgg agctttcgga ccgcgtgact tccggttcat cattaatgcc
    31081 gcagaagaaa aacccggatg cgctggagct gattcgcggt aaatgcggcc gggtgcaggg
    31141 ggcgttaacc ggcatgatga tgacgctgaa aggtttgccg ctggcttaca acaaagatat
    31201 gcaggaagac aaagaaggtc tgttcgacgc gctcgatacc tggctggact gcctgcatat
    31261 ggcggcgctg gtgctggacg gcattcaggt gaaacgtcca cgttgccagg aagcggctca
    31321 gcagggttac gccaacgcca ccgaactggc ggattatctg gtggcgaaag gcgtaccgtt
    31381 ccgcgaggcg caccatattg ttggtgaagc ggtggtggaa gccattcgtc agggcaaacc
    31441 gctggaagat ctgccgctcg acgagttgca gaaattcagc ccggtgattg acgaagatgt
    31501 ctatccgatt ctgtcgctgc aatcgtgcct cgacaagcgt gcggcaaaag gcggcgtctc
    31561 accgcagcag gtggcgcagg caattgcttt tgcgcaggcg cggttggggt aagaacattt
    31621 atatgtataa atttgagcct ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat
    31681 cctggagatc cgcaaaagtt cacgttggct ttagttattc gagttgagaa actctcgaaa
    31741 cgggcagtga cttcaagggt taaaagaggt gccgctccgt ttctgtgagc aattatcagt
    31801 cagaatgctt gatagggata atcgttcatt gctattctac ctatcgccat gaactatcgt
    31861 ggcgatggag gatggataat gaatattcgt gatcttgagt acctggtggc attggctgaa
    31921 caccgccatt ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg
    31981 caaattcgta agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa
    32041 gtgttgttca cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag
    32101 gtgaaagtcc ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcat
    32161 attggtttga ttcccacggt tggaccgtac ctgctaccgc atattatccc gatgctgcac
    32221 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
    32281 caactggaca gtggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca
    32341 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg
    32401 tgggcgaacc gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg
    32461 gaagatggtc actgtttgcg cgatcaggca atgggtttct gttttgaagc cggggcggat
    32521 gaagatacac acttccgcgc gaccagcctg gagacgctgc gcaacatggt ggcggcaggt
    32581 agcgggatca ctttactgcc tgcgctggct gtgccgccgg agcgcaaacg cgatggggtt
    32641 gtttatctgc cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct
    32701 ggctcaccgc tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc acgaatggat
    32761 ggccatttcg ataaagtgtt aaaacaggcg gtttaaaccg tttaacgcag ctacccgata
    32821 ggcttccgcc atggtcgggt agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc
    32881 gccacctttc tgttccataa tcgcctgacc gatatgaata atttcggcag cgcgctcgcc
    32941 aaagcagtga atacccagaa tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc
    33001 cacgttcatg ccgacgattt gtgcgcgtgc cagatgttta aactgggcgc ggcccacttc
    33061 atatggcact ttcatcgcgg tcagctgctg ttcggttttg cccacagagc tgatttccgg
    33121 gatggtgtaa atgccggtag ggatatcttc aatcagatgc gcggtggctt cgccttttac
    33181 cagcgcctgc gcggcaatgc gcccctggtc ataggccgct gatgccaggc tcggataacc
    33241 aatcacgtcg cccaccgcgt aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt
    33301 cagctgtccg cgactgtcag tttccagccc aatgttctgt aacgccagcg aatcggtatt
    33361 accggtgcga ccgttggcat agagcaggca gtcagctttc agttttttac ccgacttcaa
    33421 atgcatgatc acaccatcgt cacagccttc gatcttctcg tactcttcgt tgtgacgaat
    33481 cactacgcca ctgttccaga agtgatagga gagagaatct gacatctctt gatcgagaaa
    33541 tgccagcagg cgatcgcggg tgttgatcag atccactttt acatccatac cgcggaagat
    33601 cgacgcatat tcacagccga tcactccagc accgtaaatc agcacatggc gcggttcgtg
    33661 gtgcatgcta agaattgagt cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt
    33721 tggatgatat ggacgagagc cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac
    33781 gctgccgtcc gggcaatcca gcgccaacgt atgctcgtca acaaagcgag cgtttccctg
    33841 caatatttca cagtgattac gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat
    33901 cacgttatcg gcatggttaa ggatatcggc aaaagaagag cggagcagtc gggaatggtc
    33961 gctgtaaagt gggttttgat tgaattctat aatgcggctg acggcgtgac ggagagcttt
    34021 cgacgggatg gtgccccagt gggtgcaacc gccgccaaca ttttgataac gctcgataac
    34081 tgcgacgcgc gcaccttgct taaccaggcc cattgcagcg ccttcgccgc cggggccgga
    34141 acctattact atggcatcgt aatcgtagga atgtggcatg gtagggctta cctgttctta
    34201 tacataaaag caacagaatg gtaacatttt atcgcgggta agccaattga tccccgtcat
    34261 ttatctggct atatcctgag cggcctttgc tttgtctgtt tcttactttt gccctgacgt
    34321 tttattggat ttttatcgac gatactctcc gtttaagcgg caggtttccg ctgtacgtaa
    34381 aagaaccggc caaagaattg cagtaaatat gttttattgc gttaccgttc attcacaata
    34441 ctggagcaat ccagtatgtt cattctctgg tatagtgcca gcagtacttt tggcaaggat
    34501 tcagacatcg tgatgggcgt aagagcgcaa caaaaagaaa aaacccgccg ttcgctggtg
    34561 gaagccgcat ttagccaatt aagtgctgaa cgcagcttcg ccagcctgag tttgcgtgaa
    34621 gtggcgcgtg aagcgggcat tgctcccacc tctttttatc ggcatttccg cgacgtagac
    34681 gaactgggtc tgaccatggt tgatgagagc ggtttaatgc tacgccaact catgcgccag
    34741 gcgcgtcagc gtatcgccaa aggcgggagt gtgatccgca cctcggtctc cacatttatg
    34801 gagttcatcg gtaataatcc taacgccttc cggttattat tgcgggaacg ctccggcacc
    34861 tccgctgcgt ttcgtgccgc cgttgcgcgt gaaattcagc acttcattgc ggaacttgcg
    34921 gactatctgg aactcgaaaa ccatatgccg cgtgcgttta ctgaagcgca agccgaagca
    34981 atggtgacaa ttgtcttcag tgcgggtgcc gaggcgttgg acgtcggcgt cgaacaacgt
    35041 cggcaattag aagagcgact ggtactgcaa ctgcgaatga tttcgaaagg ggcttattac
    35101 tggtatcgcc gtgaacaaga gaaaaccgca attattccgg gaaatgtgaa ggacgagtaa
    35161 tgaaacaagc aaatcaagat agaggtacgc tgctgctggc gttggttgct ggcttatcga
    35221 ttaatggtac tttcgcagcg ctgtttagct ccattgtgcc attttctgta ttcccgatta
    35281 tttccctggt gctgacggtt tactgcctgc atcaacgtta tcttaatcgc accatgccgg
    35341 taggcttgcc gggtctggca gctgcctgtt ttattctcgg cgtactgctg tacagcacgg
    35401 tagttcgtgc ggaatatccg gatatcggct ctaacttctt cccggcagta ctctccgtca
    35461 ttatggtgtt ctggattggc gcgaagatgc gtaaccgtaa gcaggaagtt gctgagtaat
    35521 cggtattatg ccggatgctg gcgcatccgg catgggtttt acttcgcggt cagtaatacg
    35581 ccgcactcca tatggtgcgt gtaggggaac tgatcaaaca gagccagacg ttcgaccttg
    35641 tgcgtctggc ttaatgtttc cagattcttg cataacgttt ccggattgca ggagatatac
    35701 agaatacgtg gatacgcctg caccattttc tcggtttcac tgtccagacc gctgcgcggt
    35761 gggtcgacaa aaatggtttc gcactgataa ctcttcaagt ctatcccttg caggcgatta
    35821 aactcgcgta caccattcat cgcctgagta aattcttctg ccgccatacg aataatttgt
    35881 acgttatcaa tatggttagc tgcgatgttg tattgcgcag cagcaaccga cggcttagcg
    35941 atttcggtgg ctaatacccg atcaaaattg cgcgccagtg ctaatgaaaa gttaccgttg
    36001 ccgcagtaca gctccagtaa atcgcctttt gagcctttgg ttacgtccag cgcccattcc
    36061 agcatctgaa tattcatcgc cgcgttcggc tgggtaaaac tgttttctac ctgacggtag
    36121 atcatctctt tccctgcgac cggcagacgt tcatcgatgt aatcttgatc cagctcgatt
    36181 ttggttttcg ttgcccgacc aatcagatgc acattcagat tctgcgcgcg cagtgcatcg
    36241 cgcagggctt ccgcttcctg acgccactca tcatccagct ttttatggta caacagggaa
    36301 accaccgcct gattactcag cgtggtgagg taatcaatct ggaacaactt gtggcgcaga
    36361 acgggattat tacgcacacc cgcaatcatc gccgtcatca actggttgat gagttcactg
    36421 gcagcgggga agctatccac gcggatgcgg cttttggttt gttgatcgaa aatgatgtga
    36481 tacaggtcat cgccatcgtg ccagatgcgg aactccgcac gcatccggta atgactgacc
    36541 ggcgagcgaa acacttccgg aaccaggtca gaaaacggtg ccatcatact ttgcaaacgt
    36601 accacttttt cggctaactg cgcttcatac tgttctgttg gaaggtgttc gggggtcatg
    36661 atgtatcctg aaaaattaaa gtacgcggcg attgtaggga ttgctcatca gatgtccaga
    36721 tcttgatgaa ttcctatttg tgagctacgt ctggacagta acttgttaca acctgtagca
    36781 tccacttgcc ggtcctgtga gttaataggg aatccagtgc gaatctggag ctgacgcgca
    36841 gcggtaagga aaggtgcgat gattgcgtta tgcggacact gccatccggt gggaagtcat
    36901 catctcttag tatcttagat acccctccaa gcccgaagac ctgccggcca acgtcgcatc
    36961 tggttctcat catcgcgtaa tattgatgaa acctgcggca tccttcttct attgtggatg
    37021 ctttacaatg attaaaaaag cttcgctgct gacggcgtgt tctgtcacag ccttttccgc
    37081 ttgggcacag gataccagcc cggatactct cgtcgttact gctaaccgtt ttgaacagcc
    37141 gcgcagcact gtgcttgcac caaccaccgt tgtgacccgt caggatatcg accgctggca
    37201 gtcgacctcg gtcaatgatg tgctgcgccg tcttccgggc gtcgatatca cccaaaacgg
    37261 cggttcaggt cagctctcat ctatttttat tcgcggtaca aatgccagtc atgtgttggt
    37321 gttaattgat ggcgtacgcc tgaatctggc gggggtgagt ggttctgccg accttagcca
    37381 gttccctatt gcgcttgtcc agcgtgttga atatatccgt gggccgcgct ccgctgttta
    37441 tggttccgat gcaataggcg gggtggtgaa tatcatcacg acgcgcgatg aacccggaac
    37501 ggaaatttca gcagggtggg gaagcaatag ttatcagaac tatgatgtct ctacgcagca
    37561 acaactgggg gataagacac gggtaacgct gttgggcgat tatgcccata ctcatggtta
    37621 tgatgttgtt gcctatggta ataccggaac gcaagcgcag acagataacg atggtttttt
    37681 aagtaaaacg ctttatggcg cgctggagca taactttact gatgcctgga gtggctttgt
    37741 gcgcggctat ggctatgata accgtaccaa ttatgacgcg tattattctc ccggttcacc
    37801 gttgctcgat acccgtaaac tctatagcca aagttgggac gccgggctgc gctataacgg
    37861 cgaactgatt aaatcacaac tcattaccag ctatagccat agcaaagatt acaactacga
    37921 tccccatttt ggtcgttatg attcgtcggc gacgctcgat gagatgaagc aatacaccgt
    37981 ccagtgggca aacaatgtca tcgttggtca cggtagtatt ggtgcgggtg tcgactggca
    38041 gaaacagact acgacgccgg gtacaggtta tgttgagaat ggatatgatc aacgtaatac
    38101 cggcatctat ctgaccgggc tgcaacaagt cggcgatttt acctttgaag gcgcagcacg
    38161 cagtgacgat aactcacagt ttggtcgtca tggaacctgg caaaccagcg ccggttggga
    38221 attcatcgaa ggttatcgct tcattgcttc ctacgggaca tcttataagg caccaaatct
    38281 ggggcaactg tatggcttct acggaaatcc gaatctggac ccggagaaaa gcaaacagtg
    38341 ggaaggcgcg tttgaaggct taaccgctgg ggtgaactgg cgtatttccg gatatcgtaa
    38401 cgatgtcagt gacttgatcg attatgatga tcacaccctg aaatattaca acgaagggaa
    38461 agcgcggatt aagggcgtcg aggcgaccgc caattttgat accggaccac tgacgcatac
    38521 tgtgagttat gattatgtcg atgcgcgcaa tgcgattacc gacacgccgt tgttacgccg
    38581 tgctaaacag caggtgaaat accagctcga ctggcagttg tatgacttcg actggggtat
    38641 tacttatcag tatttaggca ctcgctatga taaggattac tcatcttatc cttatcaaac
    38701 cgttaaaatg ggcggtgtga gcttgtggga tcttgcggtt gcgtatccgg tcacctctca
    38761 cctgacagtt cgtggtaaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg
    38821 ctaccaaact gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc
    38881 ccaccgtgct ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc
    38941 atctcttacc ggatctccat tacatttatg ctttcgataa cgtcgctttt ccgtatggcg
    39001 aaaaaagcga agagtttatt gttgagcgag tggtggcaat tgtcaccgca gtgcaagaac
    39061 gttatcccct tgcgctggct gtggtcgctt gcaacaccgc cagtaccgtt tcacttcctg
    39121 cattacgcga aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg
    39181 cacgtctgac ggcaaatggc attgtcggat tactggcaac ccgcggaact gttaaacgtt
    39241 cttatactca tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct
    39301 cggcagagat ggttgagttg gctgaagcga agctgcatgg cgaagatgtt tctctggatg
    39361 cactaaaacg tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat
    39421 tgggttgcac ccatttccct ctactacaag aagaactctt acaggtgctg ccagagggaa
    39481 cccggctggt ggattctggc gcagcgattg ctcgcagaac ggcctggctg ttagaacatg
    39541 aagctccgga tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag
    39601 aagctgaaca attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg
    39661 cagttttagg ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct
    39721 tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac
    39781 gccgccgggt cagcggggtt ctcctgagaa
//
LOCUS       DAMZII010000030        38929 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZII010000030 DAMZII010000000
VERSION     DAMZII010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.030"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            122..1375
                     /locus_tag="POA77_001527"
     CDS             122..1375
                     /locus_tag="POA77_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6364987.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMATALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1772..2107
                     /locus_tag="POA77_001528"
     CDS             1772..2107
                     /locus_tag="POA77_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDI6364988.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            2200..2283
                     /locus_tag="POA77_001529"
     CDS             2200..2283
                     /locus_tag="POA77_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6364989.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2383..3204
                     /locus_tag="POA77_001530"
     CDS             2383..3204
                     /locus_tag="POA77_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6364990.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3243..3572)
                     /gene="mdtI"
                     /locus_tag="POA77_001531"
     CDS             complement(3243..3572)
                     /gene="mdtI"
                     /locus_tag="POA77_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDI6364991.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3559..3924)
                     /gene="mdtJ"
                     /locus_tag="POA77_001532"
     CDS             complement(3559..3924)
                     /gene="mdtJ"
                     /locus_tag="POA77_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDI6364992.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4336..5370
                     /gene="tqsA"
                     /locus_tag="POA77_001533"
     CDS             4336..5370
                     /gene="tqsA"
                     /locus_tag="POA77_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDI6364993.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5395..6783)
                     /gene="pntB"
                     /locus_tag="POA77_001534"
     CDS             complement(5395..6783)
                     /gene="pntB"
                     /locus_tag="POA77_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDI6364994.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6794..8326)
                     /gene="pntA"
                     /locus_tag="POA77_001535"
     CDS             complement(6794..8326)
                     /gene="pntA"
                     /locus_tag="POA77_001535"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDI6364995.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8850..9794
                     /locus_tag="POA77_001536"
     CDS             8850..9794
                     /locus_tag="POA77_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6364996.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9980..11362
                     /locus_tag="POA77_001537"
     CDS             9980..11362
                     /locus_tag="POA77_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6364997.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11399..12121
                     /gene="folM"
                     /locus_tag="POA77_001538"
     CDS             11399..12121
                     /gene="folM"
                     /locus_tag="POA77_001538"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDI6364998.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12118..12453)
                     /locus_tag="POA77_001539"
     CDS             complement(12118..12453)
                     /locus_tag="POA77_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDI6364999.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12582..13301
                     /gene="rstA"
                     /locus_tag="POA77_001540"
     CDS             12582..13301
                     /gene="rstA"
                     /locus_tag="POA77_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDI6365000.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13305..14606
                     /gene="rstB"
                     /locus_tag="POA77_001541"
     CDS             13305..14606
                     /gene="rstB"
                     /locus_tag="POA77_001541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDI6365001.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14682..15611
                     /gene="tus"
                     /locus_tag="POA77_001542"
     CDS             14682..15611
                     /gene="tus"
                     /locus_tag="POA77_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDI6365002.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15608..17011)
                     /gene="fumC"
                     /locus_tag="POA77_001543"
     CDS             complement(15608..17011)
                     /gene="fumC"
                     /locus_tag="POA77_001543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI6365003.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMFEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGR"
     gene            complement(17154..18800)
                     /gene="fumB"
                     /locus_tag="POA77_001544"
     CDS             complement(17154..18800)
                     /gene="fumB"
                     /locus_tag="POA77_001544"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI6365004.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18999..20174
                     /gene="manA"
                     /locus_tag="POA77_001545"
     CDS             18999..20174
                     /gene="manA"
                     /locus_tag="POA77_001545"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDI6365005.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20275..21783
                     /locus_tag="POA77_001546"
     CDS             20275..21783
                     /locus_tag="POA77_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDI6365006.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21828..23093)
                     /gene="uidC"
                     /locus_tag="POA77_001547"
     CDS             complement(21828..23093)
                     /gene="uidC"
                     /locus_tag="POA77_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDI6365007.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23132..24505)
                     /gene="uidB"
                     /locus_tag="POA77_001548"
     CDS             complement(23132..24505)
                     /gene="uidB"
                     /locus_tag="POA77_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDI6365008.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24502..26313)
                     /gene="uidA"
                     /locus_tag="POA77_001549"
     CDS             complement(24502..26313)
                     /gene="uidA"
                     /locus_tag="POA77_001549"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDI6365009.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(26702..27292)
                     /gene="uidR"
                     /locus_tag="POA77_001550"
     CDS             complement(26702..27292)
                     /gene="uidR"
                     /locus_tag="POA77_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDI6365010.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27521..28288)
                     /gene="hdhA"
                     /locus_tag="POA77_001551"
     CDS             complement(27521..28288)
                     /gene="hdhA"
                     /locus_tag="POA77_001551"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDI6365011.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28400..29428)
                     /gene="malI"
                     /locus_tag="POA77_001552"
     CDS             complement(28400..29428)
                     /gene="malI"
                     /locus_tag="POA77_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDI6365012.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29603..31195
                     /gene="malX"
                     /locus_tag="POA77_001553"
     CDS             29603..31195
                     /gene="malX"
                     /locus_tag="POA77_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDI6365013.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            31205..32377
                     /gene="malY"
                     /locus_tag="POA77_001554"
     CDS             31205..32377
                     /gene="malY"
                     /locus_tag="POA77_001554"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDI6365014.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARRDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32481..33482
                     /gene="add"
                     /locus_tag="POA77_001555"
     CDS             32481..33482
                     /gene="add"
                     /locus_tag="POA77_001555"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDI6365015.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSSVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(33517..34557)
                     /locus_tag="POA77_001556"
     CDS             complement(33517..34557)
                     /locus_tag="POA77_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6365016.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34800..34925
                     /gene="blr"
                     /locus_tag="POA77_001557"
     CDS             34800..34925
                     /gene="blr"
                     /locus_tag="POA77_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDI6365017.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            35198..35413
                     /gene="ydgT"
                     /locus_tag="POA77_001558"
     CDS             35198..35413
                     /gene="ydgT"
                     /locus_tag="POA77_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDI6365018.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            35499..35939
                     /locus_tag="POA77_001559"
     CDS             35499..35939
                     /locus_tag="POA77_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDI6365019.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            36016..36597
                     /gene="rsxA"
                     /locus_tag="POA77_001560"
     CDS             36016..36597
                     /gene="rsxA"
                     /locus_tag="POA77_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDI6365020.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            36597..37175
                     /gene="rsxB"
                     /locus_tag="POA77_001561"
     CDS             36597..37175
                     /gene="rsxB"
                     /locus_tag="POA77_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDI6365021.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            37168..>38929
                     /gene="rsxC"
                     /locus_tag="POA77_001562"
     CDS             37168..>38929
                     /gene="rsxC"
                     /locus_tag="POA77_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDI6365022.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAIQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPR"
BASE COUNT         9477 a        10063 c         9515 g         9874 t
ORIGIN      
        1 aagttcaata ttcatattta aaacatctta tttgagatta ttaatatatt agacagaaca
       61 attcgatttt cctaacctat gtataagcct gatccacagg catatttagc aaggatttca
      121 agtgagccgt actacaactg ttgatggcgc tccggcaagc gacactgaca agcaaagcat
      181 ttctcagcca aatcaattta ttaaacgcgg tacgccgcaa tttatgcgcg tcaccctggc
      241 gctgttctct gccggactgg caacatttgc acttctctat tgtgtgcagc ctatccttcc
      301 ggtgctttcg caggagtttg gcttaacgcc cgcgaacagt agtatttcac tgtccatttc
      361 cacggcgatg ttggctattg gtttgctgtt tactggcccc ctatccgatg ccattggtcg
      421 caaaccagtg atggccacgg cgctactgtt ggcctccatt tgtacgttac tttcgacaat
      481 gatgaccagc tggcacggca ttttgattat gcgcgccttg attgggcttt cgttaagtgg
      541 cgtggcagct gttggcatga cttatcttag cgaggaaatc caccccagtt tcgtggcctt
      601 ttcgatgggg ttgtatatca gcggcaactc aattggcggc atgagcgggc gcttaattag
      661 tggtgtcttc acggactttt tcaactggcg aattgctctg gcggcaatcg gttgtttcgc
      721 gctagcctcg gcgttgatgt tctggaaaat cctccctgaa tcacgccatt ttcgcccgac
      781 ttcgctgcga cctaagacgt tgtttatcaa ctttcgtctg cactggcgtg accggggatt
      841 accgttattg ttcgcagaag gctttttgct gatggggtcg ttcgtcacgc tgtttaatta
      901 catcggctat cggttgatgc tttccccctg gcatgtcagt caggccgtgg ttggcttatt
      961 atcgctggct tatttgaccg gtacatggag ctcacccaaa gccggaacca tgaccacccg
     1021 ctacgggcgc ggtccggtga tgttgttttc gacgggggtt atgctgtttg gtttactgat
     1081 gaccttattc agctcgctgt ggctgatctt tgccggaatg ttactcttct cagcaggatt
     1141 cttcgcagcc cactcagttg ccagcagctg gattggcccc cgcgcaaaac gcgctaaagg
     1201 ccaggcctcc tcgctgtatc tgttcagtta ctatctgggg tcgagtattg ccgggacgct
     1261 gggtggtgtt ttctggcata actatggctg gaacggcgtc ggcgcattta ttgctctgat
     1321 gctggtcatt gctctgctgg tcgggacgcg tttgcatcgt cgtctgcacg cctgaaaaat
     1381 aagcccggac tgcggtaact acacgtccgg acttattgct tgctcaaacc aacgttaata
     1441 gccatcctaa aatagacgaa gcgccagcca attcccgcag cgcgtctagc gtcatcagga
     1501 ttataagtac ccaaataaac ggattcattt tgctgtgtgt catttattac tgatgcgcag
     1561 ttattctacc gctttgtaag tagtaaaata gttaacccga tcaagactac tattattggt
     1621 agctaaattt cccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt
     1681 gtacatatcg ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat
     1741 agttatttca acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat
     1801 gaaaaaagta ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc
     1861 tgcagagact gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccggcgaa
     1921 aacgacacat cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa
     1981 aaagcatcat aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc
     2041 gaagaaacac gccgggaaac acagccatca gcaaccggca aaacctgctg cacaacccgc
     2101 agcgtaagtt ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta
     2161 agcgggtaac gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt
     2221 gagttcattc ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc
     2281 tgactgtagt tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga
     2341 gggtttgctt ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct
     2401 gtagtgttgg gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt
     2461 gccaaatcgg caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca
     2521 gtgaatgaca cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc
     2581 aatttatgta cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta
     2641 ttgacacctc caaagggtaa agccgataaa gcagtggcgc tgcgttttgt gtcaaataaa
     2701 ggtctttggc gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag
     2761 cggttaaaag cagatgggga tggttggatt gttcctcccg cagctgcacc atgggacttc
     2821 ggcttgattg tgctgcgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga
     2881 gataaagccg cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga
     2941 tatcctgaag atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg
     3001 gcgcaaacgt cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct
     3061 ctgatgttgc ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc
     3121 gcgaaagatc gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag
     3181 ctggatcaac tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt
     3241 catcaggcaa gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct
     3301 ttacgattta accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca
     3361 aacccgcccc acaatgcata agcgacagac aagtcgatcc cttttacagc ctgagaaagc
     3421 gcactaaagg cagccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag
     3481 ccgtcagaaa atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc
     3541 caggcggcgt gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc
     3601 tttacgggta cctgatttga tcaacacaat cccggcgacc agggtggtta acccggcaat
     3661 tttcatcagc gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat
     3721 accgatacct tcccacagcg cataagctac gcctaaggcg atctttttaa cggcgaaaga
     3781 gagaaatata tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc
     3841 actgacgctc gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccaggcc
     3901 taataaaatc caataaatat acattgtcct tctcctgcaa gagaattatt ttaattttcg
     3961 cttaattcag cgaaaccaaa gtaaagtggt caagccccgc gcatagggca agcttaggca
     4021 gaagaaagga ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg
     4081 aggtacgaag atgggtaaat gttgaaaata acgtcctgaa caaattgtcc ataatattac
     4141 aattatccgc agtgttgctt ctcgtcatcg cggatgataa ttgtcctcgg tagttgaaca
     4201 cgcctgattt gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag
     4261 cggtttgatt ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa
     4321 aaaacgggga caagcatggc aaagccgatc atcacgctca atggcctaaa aatcgtcatt
     4381 atgttgggaa tgctggtcat tattctctgc ggtatccgtt ttgccgccga gatcatcgtg
     4441 ccgtttattc tcgcattatt tattgctgtt attcttaacc cgctggtgca acacatggtc
     4501 cgctggcgag tgccgcgtgt actggcggtg tcgattttga tgaccatcat cgtgatggcg
     4561 atggtgttgc tattagctta tctgggttcc gcgctcaacg agttgacgcg gacgttaccg
     4621 caatatcgca actctattat gacgccgctg caagctcttg agccgttgtt gcaacgcgta
     4681 gggattgacg tctcagttga ccagctggcg cattacattg atccgaacgc ggcgatgacg
     4741 ttgctcacca acttattgac gcagttatct aatgccatgt catcaatatt tttattgctg
     4801 ctgacggtgc tgtttatgct gctcgaagtg ccacaattgc ccggaaaatt tcagcaaatg
     4861 atggcgcgtc cggttgaagg gatggcggcg attcaacgtg caattgacag tgtttctcat
     4921 tatctggtgc tgaaaacagc catcagcatc atcaccggcc tggtcgcctg ggcgatgctc
     4981 gccgcactcg atgttcgctt cgcttttgtc tggggattgc tggcctttgc gcttaattac
     5041 atccctaata ttggttcagt tctcgcggca atccccccta tcgctcaggt actggtgttt
     5101 aatggcttct acgaagcgtt gctggtgctg gcgggatatc tgctgattaa tctggtcttc
     5161 ggcaatattc tggagccgcg catcatggga cgtgggctgg ggctttccac attggtggta
     5221 tttttgtcgt tgattttttg gggatggttg ttaggaccgg tgggtatgct gctttccgtg
     5281 ccgttaacaa ttattgtcaa aattgcgctt gaacaaacag cgggaggtca aagcatcgcc
     5341 gttctgttaa gcgatctcaa taaagagtga cggcctccgc agaggccgtc agggttacag
     5401 agctttcagg attgcatcca cgctggcttt ggcgtcacca aacagcatgt gggtgttttc
     5461 cttgaagaac agcgggtttt gcacaccagc atagccagtg ttcatcgaac gtttaaagac
     5521 aatcacgttc tgcgctttcc acacttccag cacaggcata ccagcaatag gactcttcgg
     5581 atcatcctgc gccgccgggt taaccgtatc gttagcacca atcaccagta cggtatcggt
     5641 atcagcaaag tcatcgttga tctcgtccat ttccagcacg atgtcatacg gtacttttgc
     5701 ttcagccagc aatacgttca tatgtccagg caaacgcccc gcaaccgggt ggataccgaa
     5761 acgcacgttg ataccacgag cgcgcagttt ctcggtaatt tcagcgacag gatattgcgc
     5821 ctgcgcgact gccatgccgt accccggagt aatgatcact gaatgggagt ttttcagcag
     5881 ttccgctgtc tcttctgcgg tgatttcgcg gtgctcgccc acttcctgat catcgccagt
     5941 agaagagccg tcggtgccga aaccacccgc aataacgctg ataaaggaac ggttcatcgc
     6001 cttacacata atgtaagaaa ggatagcgcc cgaagaaccg accagcgcac cggtcacaat
     6061 cagcaggtcg ttgctgagca taaagcccgc agccgcagcc gcccagccgg agtacgagtt
     6121 cagcatcgac accaccactg gcatatctgc accaccgatg gaggcgacta aatgccagcc
     6181 gaataccagc gcaatggcgg tcattatcag caatgccagc acttgcaggc cgacgctatc
     6241 cgtgcgaaca aatacaatca gcagcaggaa ggaaacgacc agagccgcca ggttcatttt
     6301 gtgacggttt ggcagcatca acggtttaga cgaaatcttg ccacacagtt tgccgaacgc
     6361 caccaccgaa cccgtgaacg ttaccgcccc gatgaagata ccgaggaaca cttccgtcag
     6421 gtgaatattg accagaatcg gtgccattcc cgcgtcatga tgcagataac tgttaaagcc
     6481 aaccagcact gccgccagac ccacgaagct atgcaggatc gccaccagtt ctggcatttc
     6541 ggtcatttca actttcttcg ccagacggat accaattgcc ccaccaatga ccatcgccag
     6601 caagatccag ccaacattac ccgtatccgg tccaaaaatg gttgcgatta acgcaatcgc
     6661 catcccggcg ataccgaagt tgttaccctg gcgagacgtt tcatgtttcg aaagaccggc
     6721 cagactgaag ataaacagga tcgcggcaac aatgtatgca gctgtaacta atcctccaga
     6781 catatgttac cccttaattt ttgcggaaca ttttcagcat gcgctgagtc acggtgaagc
     6841 caccgaaaat attaatgctg gctataagca ccgcgataaa actaaggaag ctaacccagc
     6901 cgccctggcc aatctgcaac agtgctccga caacaataat ccctgaaatc gcgttggtga
     6961 ccgacatcaa cggtgtatgc agcgcatgcg atacattcca caccacgtaa taaccgacaa
     7021 cgcaggccag cgcgaaaacg gtgaagtgcc caagaaactc tttcggcgca acgcttgcca
     7081 gccaaccgaa aaggatgatt gccagcgcca tcaatgcgta tttacgccac ggtgagcagg
     7141 cacatttttc ctcagttttc acttccggtg ccgctttttg tgccgcctgc ggctgagctg
     7201 atacctgaat cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg gtcacgccgc
     7261 gaatcaccac atcatcaaaa tcaacagtga tattgccgtc tttctctttg cacaacagtt
     7321 tcagcagatt aacgaggtta gtaccgtaaa gctgtgagga ttgcgtaggc agacggcccg
     7381 gaagatcggt ataaccaatc actttgacac cattttccgt agtgaagatt tcacccggca
     7441 cggtgtattc acagttgcca ccgttttggg ctgccaggtc gacaatcaca ctgcccgcct
     7501 tcatggagtc aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg
     7561 cggtggtgac aatgatatcg acctcttttg cctgggcggc aaagagttcc atttccgctt
     7621 tgatgaacgc gtccgacatc actttggcat agccatcgcc gctgcccgct tcctctttaa
     7681 aatccagctc gaggaattcc gcgcccatac tttgaacttg ttctttcact tccgggcggg
     7741 tgtcgaatgc acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccagacctg
     7801 caacacccgc accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtaattt
     7861 gcccggtaaa gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga
     7921 tgttcgccat cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagagt
     7981 ccatcgccat cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg
     8041 caggccagat aaaactcacc agcgttgtcc caggattcag taacgcaatt tcatcatcta
     8101 acggcgcatt gaccttcaga atgatctctg actgccagac gctattccct tctacaattt
     8161 cagcgcccgc ttgcacaaac gctttatcgt caaaacttgc cagttgaccc gcgccgctct
     8221 ctaccgcgac ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa
     8281 cacgggtttc attggttaac cgttctcttg gtatgccaat tcgcatgata ttcccttcca
     8341 tcggttttat tgatgatggt ttgcctgtgt caggagccac acaagccgct catgtacgag
     8401 ctaaatgtta ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta
     8461 tgcctgtgat ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat
     8521 tcagccgtga tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc
     8581 gacggtcggg taaaacgaga catcgccccg gcacgaatca ctacttaaca ttaaattaac
     8641 ttatacaatt cagttgcttc agtagtaatg atgctgatac ggctgttttt taagcataga
     8701 cggtcatttg agcaggatta aaattggctt aaggaatgtg atatgaaaaa tgacgcagac
     8761 agttacaccg tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc
     8821 tggtttgcgc aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg
     8881 cactgctttc tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag
     8941 cggcagcggt taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg
     9001 aagcggtgaa agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg
     9061 tcgacacttc tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag
     9121 ccgatgctga aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt gtcgaactgc
     9181 cgaaagatca ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc
     9241 gtagccagcc agaagtcaat gatgccatca ccaaagcagc aaaagcgaaa ggtgcctact
     9301 ctttctacat tgttcgtcaa atcgatgcca accagggcgg caaccagcgt attactgcat
     9361 tcatctataa aaaagatgct aagaaacgta tcgtccagag cccggatgtg atcccggcag
     9421 attccgaagc aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga
     9481 tcccaggtgt tgcgactacc gcatcaccaa gttctgaagt cggtcgtttc tttgaaaccc
     9541 agtcatcaaa aggcggacgt tacactgtca cgcttcctga cggcactaaa gtcgaagaac
     9601 tgaacaaagc gacagcagcg atgatggtcc cgttcgacag catcaaattc tctggcaact
     9661 acggcaacat gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta
     9721 agtattacca catcacccgc cagtggcagg aacgtggtaa taacctgacc gtcagcgcag
     9781 atctgtataa ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt
     9841 tttagtcaca aatttccccg cgaccattgc atgcagacac atctcttcgt aaaatcccgc
     9901 gcttcagtgg caaactccat ttttatgcgt tttgccctaa tatttattct tcttatcacg
     9961 ttttaatcac tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt
    10021 attaagctca atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc
    10081 cagcccggca gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc
    10141 ctttgccatg ctgatcctca cgcgcattcg tcccgaactt gacggcggta tctttaccta
    10201 tgcccgcgaa gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg
    10261 cgcagtcatc gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac
    10321 ggacacgccg gaattgcgcc tgttcggcga tggtaatacc tggcaatcga tagtcggtgc
    10381 atcggcttta ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag
    10441 cattaacctg gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg ttgtgctggc
    10501 gatgatgatg ttcaaactgg ataccttcaa gctcgacttc accggacttg cacttggcgt
    10561 acccgtgtgg gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt
    10621 ggaaggtgcc gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg gcaaagcgac
    10681 actgctggcg gttctctccg ctctgggcgt ttacttgctg gtaacgctgc tttcgctggg
    10741 cgtggtggca cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt
    10801 ggaaatgatg ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg tttccgtttg
    10861 cggcgcgtac ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg ctgccactca
    10921 taaagcattc ccacgcattt ttgcgcgcca gaacgcacaa gctgcgccat ccgcctcgct
    10981 gtggttgacc aacatctgcg tgcaaatttg tctggtactc atctggctga ccggttccga
    11041 ttacaacacg ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg
    11101 cgcgttcttg ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg tcggtgcctg
    11161 catttatggc ttatggttat tgtatgcctc tggtccaatg cacttgctgt tatccgttgt
    11221 tctgtatgcg ccggggctgc tggttttcct gtatgcgcgc aaaacgcata cacatgataa
    11281 tgtactgaac cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc
    11341 gacctggatg ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat
    11401 gggtaaagcc cagcccttgc caatattaat tactggcgga ggtcgtcgca tcggcctcgc
    11461 cctcgcctgg catttcatta atcaaaagca accggtgatt gtcagctatc ggacgcacta
    11521 tccagccatt gatggactga ttaaggcagg tgcgcagtgt attcaggctg atttttcgac
    11581 caacgacggt gtgatggcgt ttgccgatga agtactaaaa agcacccatg gtctgcgtgc
    11641 tattttgcat aacgccagtg cgtggatggc ggaaaaacca ggtacaccac tggccgacgt
    11701 actggcttgc atgatgcaga tccacgttaa taccccatac ctgctcaacc atgcgctgga
    11761 aagattactg cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt
    11821 ggtggagcgc ggtagcgaca aacatgttgc gtatgctgca agcaaagcgg cactggataa
    11881 tatgacccgc tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgcgcc
    11941 atcgctgatc ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa
    12001 atcactgatg aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact
    12061 taccagctgc tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc atctgcgtta
    12121 atgcagcttt atccagcata taatcaacac ccatgcgctt atcccccaac acgcaacaga
    12181 accaacaaaa gcggcgggca agcgcatcat tccggtgaaa taccacagcg acaccaggta
    12241 aacaaaatag ggaataatcg accacatact aaaaatgatg gttgcgcgta aggcttcaat
    12301 gccgcgttcg ctggcaacaa tataatgcgc gataagcgca aaggtcggga aaagtggaat
    12361 cagcccggcg atataataat ttttcgtttt tgctaaaaca ccaatcaaca gcactaccag
    12421 cgcaccgagc gcggctttga ttaccagccc catcttttta ccttaacact tccataacaa
    12481 gtcatcagta gaatacctga tgaaaacttg tttagaaacg attgatagta agtaaaaaca
    12541 gcgcggtgta ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact atcgtatttg
    12601 tggaagatga tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa catgatatgc
    12661 aggttaccgt agaaccgcgc ggcgaccagg ccgaagaaac cattttgcga gaaagtccgg
    12721 atttggtgtt actcgacatc atgctaccag gcaaggacgg catgaccatt tgtcgtgatt
    12781 tacgcgcaaa gtggtctgga ccgattgttc ttctaacctc tctcgatagc gatatgaacc
    12841 acatcctggc actggaaatg ggtgcctgcg actatattct caaaacgacg ccccctgctg
    12901 ttttgctagc gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca ctgaccaaag
    12961 gtcttcagga aacgtctctg actccctaca aagccctgca tttcggcacg ttgaccatcg
    13021 atcccatcaa ccgcgtagtc accctggcta acactgaaat ctcgctctcg acagctgatt
    13081 tcgaattatt gtgggaatta gctacccatg ccgggcaaat catggaccgc gatgcattgc
    13141 tgaaaaattt acgcggcgtc agttatgacg gactggatcg tagcgtggac gtggctattt
    13201 cgcggttaag aaaaaaactg ctcgataacg ccgcagaacc ttatcgcatt aaaactgtgc
    13261 gtaacaaagg ctatcttttt gcgcctcatg catgggaata agcgatgaaa aaactgttta
    13321 tccagtttta cctgttattg tttgtctgct tccttgtgat gtctctgctg gttgggttgg
    13381 tgtacaaatt taccgccgaa cgcgcgggca aacagtcgct ggatgatttg atgaacagtt
    13441 cgctgtatct gatgcgcagc gaattgcgtg agatcccccc acacgactgg ggtaagacgc
    13501 tgaaagagat ggatttaaat ctctctttcg atctgcgtgt cgagccactg agtaaatacc
    13561 atcttgatga tatttccatg caccgactgc gtggcggcga aattgtcgcc ctggacgatc
    13621 agtacacgtt tttgcagcgt atcccgcgca gccactacgt gctggcagtt ggtcctgttc
    13681 cttatcttta ttatctccat cagatgcgat tgctggatat cgccctgatc gcttttattg
    13741 ctatttccct cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa
    13801 aactggaagc agcggcgcaa cgatttggcg atgggcatct caatgagcgt atccactttg
    13861 atgagggttc gagctttgaa cgacttggta tcgcatttaa ccagatggcg gacaatatca
    13921 acgccttgat tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac
    13981 cgttagtgcg cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc
    14041 aggcgttgaa tcgtgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg
    14101 cccgactcga tcgcccacaa aacgagcttc atcttagcga accagacctg ccgttgtggc
    14161 tgtcaacgca tctggcagat attcaggcag taacgcccga taaaacggta cggataaaaa
    14221 cgctcgtgca aggccattat gcggcgttgg atatgcgctt aatggagcgc gtgctggata
    14281 atttgctcaa taacgccctg cggtactgcc attcaacggt tgaaaccagc ctgctactgt
    14341 cggggaatag agcgacatta attgttgagg atgatggccc agggattgcc ccagaaaacc
    14401 gcgaacatat ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc tcaaccggcg
    14461 gctgcgggct ggggctggca attgtccact ctatagcact ggcaatgggc ggtacggtta
    14521 attgtgacac cagcgaactg ggtggtgccc gcttctcgtt tagctggccg ttatggcata
    14581 acatcccgca atttacctct gcctgacatt acgcgcacga tggtcaagtc accaccactg
    14641 tgctataaaa taagtatgtt gtaactaaag tggttaatat tatggcgcgt tacgatctcg
    14701 tagaccgact caacactacc tttcgccaga tggaacaaga gctggctgca tttgccgctc
    14761 atcttgagca acacaagcta ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag
    14821 aggatgagca taatccgctt aatcgtattg aggtgaaaca acatctcggc aacgacgcgc
    14881 agtcgctggc gttgcgtcat ttccgccact tatttattca acaacagtcc gaaaatcgca
    14941 gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc
    15001 aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata
    15061 tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg catcgtcatt
    15121 tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca
    15181 ctttacgctt tggttgggct aataaacata tcattaagaa tttgcatcgt gatgaagtcc
    15241 tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg
    15301 agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca cagaacgcga
    15361 agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag
    15421 atcaaaaaca agtccaacac gcctgcccta caccgctgat tgcactgatt aatcgggata
    15481 atggcgcggg cgtgccggac gttggtgagt tgttaaatta cgatgccgac aatgtgcagc
    15541 accgttataa accacaggcg cagccgcttc gtttgatcat tccacggctg cacctgtatg
    15601 ttgcggatta acgcccggct ttcatactgc cgaccatctg ttctggccgt acccagcggt
    15661 caaactcggc ttcgctaaga taccccagcg caagggccgc cgcttttaag gtcagccctt
    15721 ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg gtgttaagcg
    15781 cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga ttaggttcaa
    15841 tgcccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt
    15901 gcaggaaatt atggatcacc atcggacgga agacgttcag ttcaaagtta ccggaagcgc
    15961 cacccatgtt gatcgccacg tcgttcccca ttacctgaca gcagagcatg gttaatgctt
    16021 cacactgggt tgggttcact ttccccggca tgattgagct gcccggctca ttttccggga
    16081 ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcga
    16141 ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agggcatcac
    16201 aggtcgccag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg
    16261 ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca gtacccaccg
    16321 ctgtaccgcc aagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat
    16381 tatgctcgaa catcgctacc cagccggaaa tctcctgccc cagtgttagc ggcgtggcgt
    16441 cctgcaagtg ggttcgaccg atttttacga tatcggcaaa tgcacgcgat ttttcactca
    16501 gcgtctgtgt cagggtttta agctgcggaa tgagttgctt gcgcagcgcc agcagcgccg
    16561 caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttc acgtcgtcgt
    16621 tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg gcccggttag
    16681 ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc cagatagcca
    16741 gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc
    16801 tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct gcgcgcttgg
    16861 ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct
    16921 ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca atcgccccca
    16981 tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgtt taattttttc
    17041 tttctgtttt gctttcgtta agcaactttt agctcactta ttatttacta tttgataaca
    17101 aatgtttagt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc
    17161 acacagcgag tgcactgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc
    17221 acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt
    17281 tccggatatt caacacattc caggctctta atacttccct gcgccaatac agcggccgga
    17341 ccaccgatac tgccaaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg
    17401 ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca
    17461 taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc
    17521 gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg cagcccttcg
    17581 ccattatcca tccgctcttt cagcttggcg tgggcaatat cacgaccgac gataatcgtg
    17641 ccattcagcg acaagcgcgt agaaacggga tactgcgaca actgtgcgag gatctctttc
    17701 atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttacg cagctcttca
    17761 gggatatatt tacctggatt atgttccagt ttttcgatcc agatcccctg acggttgatc
    17821 ttcgctttga tattacggtc agcagagcag gaaacaccca taccaaccgg acaggatgcg
    17881 ccgtgacgtg gcaggcgaat cacgcggatg tcgtgagcga agtatttacc accaaactgc
    17941 gcacccagac cgagattttg cgcttcgatc agcaattctt tttccagttc cacatcgcgg
    18001 aacgcctgac cgtgctcatt cccttccgtt ggcagttcat catagtattt cgcggaagcc
    18061 agtttcaccg ttttaaggtt cgtttctgca gaagttccac caataacgaa cgcaatatga
    18121 tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta atttttcagt
    18181 ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt tgccgaacca
    18241 ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata aagatcgatc
    18301 tgcgctggta gattggtgcc agtattcacc tctttataca tatccagcgg cgcgttttgc
    18361 gagtagcgca gattatcttc gatataagtg ttatagacac cgcgcgccag cgccgcttca
    18421 tcaccaccac cggtccatac acgctgccct tttttaccaa caataatcgc agtgccggta
    18481 tcctgacagg ttggcagaac acctttcgcc gcgatgtcgg agttgcgcag gaattgcagc
    18541 gccacatatt tatcattttc gctggcttcc gggtcacgca gaatgtcggc cacctgttgt
    18601 tggtgagccg gacgcagcat aaatgacgca tcatgaaacg cctgacgcgc caacagagtt
    18661 aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt
    18721 tcgctggtta gcaggtaata ctcagtatca tcttttttga gtggaaaagg agcctgataa
    18781 tgaaagggtt tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc
    18841 gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt tttaacaaaa
    18901 actgagacta gtacgacttt ttgcggctcc aggttacttc ccgtaggatt cttgctttaa
    18961 tagcgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag
    19021 tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc
    19081 cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacgag
    19141 tgcagaatgc cgctggagat atcgtttcac tgcgtgatgt gattgagagt gataaatcga
    19201 ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat
    19261 tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacacaat tctgaaatcg
    19321 gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag
    19381 atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt
    19441 ttcgtgagtt ttccgagatt gtctccctac tccagccagt cgcaggtgca catccggcaa
    19501 ttgctcactt tttacaacag cctaatgccg aacgtttaag cgaactgttc gccagcctgt
    19561 tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gcgctcgaca
    19621 gtcagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca
    19681 gcggcctgtt ctccccgctg ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt
    19741 tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga
    19801 actccgataa cgtgctgcgt gcgggtctga cgcctaagta cattgatatt ccggaactgg
    19861 ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac
    19921 aaggtgcaga actggacttc ccgattccag tggatgattt tgccttctcg ctgcatgacc
    19981 ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc tgcgtcgaag
    20041 gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt
    20101 ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt
    20161 acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc
    20221 ttataacacc ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat
    20281 aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca
    20341 tggtatacag gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg
    20401 caactcaaac tgacagctcc tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc
    20461 ggcgtattca gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg
    20521 tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg
    20581 cttgcccagc ttaaaaaact gaacctgatc ccgtcgatgg catcaattca aaccacgctg
    20641 gttaataacg aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag
    20701 attaactcgc gcattggtta cagcggtgat tccagttccg atatttcgct caagccactg
    20761 aattacgagc aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgcg
    20821 gacagagacg gcaaagctat ctccctttcc ggggaggcgc aaagtggtcg gatagacgca
    20881 gttaacgaat acaaccagaa agtgcagttg acctttaata atctgaaaac cgacggttcc
    20941 agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg
    21001 accatttcgg tggaaggcaa agaactggca ctgctggaag gcatggagat cagcggtaaa
    21061 tcggatctgg tcaatgatgg taaaacgatc aatagccaac tggattactc gctaaacagc
    21121 ctgaaggtac agaatcagga tctgggcagc ggcaagctga ctttaaaagt cggccagatt
    21181 gatggtgaag cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg
    21241 gcacagccag aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc
    21301 tttagcgccc tgccgctgat gttgaaaggc gatccggtga ttactatcgc gccgctaagc
    21361 tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca
    21421 acgactaaag aagcgccgca aacgctggcg caggaagtag atcgttcggt taaatctctg
    21481 gatgcgaaac tgaccattcc ggtggatatg gcaactgagt ttatgactca ggtagcgaag
    21541 ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca
    21601 tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc
    21661 ctgcaatata ctaacggtca gataacgtta aacgggcaga aaatgccact ggaagatttc
    21721 gttggtatgt ttgcaatgcc ggcattaaat gttccggtcg tacccgctat tccgcagcag
    21781 taattcacag ccaaaacgcc ggagattttt ctctccggcg ttatttttta cttcagcata
    21841 aagtcatact gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg
    21901 ccatcgcgct tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc
    21961 ccttcgctga cccgatagtt cataccacct aagatacccg cgccttcaat ccgcacatct
    22021 cgtttactgg attcatagcc gcgagtcaca atcggtgcaa atatcagtgt aaattgcggc
    22081 gttaaacgat agttgacgcc caattgccag gactgcattc cttcacggtt acgatcaaga
    22141 cttaatgaaa agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc
    22201 aaataaccac cactgccaaa tacgcttccc cacggcgcat tccaggtcaa ttgtccacta
    22261 accaacgccg tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa
    22321 aagccgagca actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga
    22381 atactttgta tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg
    22441 gtatcatcat gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc
    22501 gtctggctgt tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat
    22561 actcgtgaac gccacattcc ccacagatca atatttggtg ttaaagccca ataacctaat
    22621 gctccttcag aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga
    22681 taaggtaagc taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt
    22741 tcactgggtt ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga
    22801 taccaccggg tactcatatc agcacgagca cctaatttat aaacataata cgccccgcct
    22861 tcaacgccaa taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc
    22921 catgcgtaaa tatctcttcc cgcgccagca ctgggcttat ccgccctgcg caattcgttt
    22981 ttcagtctga tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcata agcagaggta
    23041 aggccagagg cagccgtcag gcaaataacg gccatggcca ctatttttct catagttgca
    23101 cctttgatgt tctgattatt tttattgaat attaattagt gatatcgctg attaattgct
    23161 gctgcacttt tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga
    23221 gcggataaaa ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa
    23281 ttgatgtgcg gatgcccata ataacttccg gcgtttgcac ctgattggcg atatatccgc
    23341 ttaatcccaa aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa
    23401 atgagaatag tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt
    23461 ctacggtatc agcttccagt gcccacatca cagtcatagt aacgccctga ccaattgaag
    23521 cgatggccaa cgcaaccaac gccaccggca gtgaccagac ggaaacccag aagaatagca
    23581 gataaccgca ggttcccagc aaagcgccaa tcaggaaggt attcttttta ccgatcctcg
    23641 cgaccatccc cggcaccagc ggtgccgatg ccacagtacc aaccaggttt tgcaccagta
    23701 ccagcacagt gaacagcccg gtatcattta acacatagcg cacgtagaac aacgacgagg
    23761 cgctgaccgc gaaggtcgaa atcagcacac acagcgcacc gatgcacaac ataaacagcg
    23821 ggcgattccg tttcagggtt tgcagactga tcttcaatga cggctgcgcc acgatacgta
    23881 ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag caccattccg gcaatcgcca
    23941 gcacaatcgt ccagaaatgg tataccgaca ccatctcttc cgggctggag ttcttaatgc
    24001 tcggtcctat cagaaatgcc aggcagacaa aggttaatga agcggcaatc ccgcgagccg
    24061 cgcccagacg ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag
    24121 gaatattcac caggctgtag caaagcccga ggcccatgta ggtcaaatat gcatacacca
    24181 ctttgctacc atggctccag tcggtcggca cccagaatac cagcacgctg aagatcatta
    24241 acggcgcagt accgaagagt aaaaacgggc ggaattttcc ccagcgggta ttcacactgt
    24301 ccaccactcg tccggcaaag acgtcggcga aggcatcgaa tacccgcacc agtaacagca
    24361 tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga
    24421 gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg
    24481 tgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt
    24541 catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc ggtcgcgagt
    24601 gaagatccct ttcttgttac ctccaacgcg caatatgcct tgcgaggtcg caaaatcggc
    24661 gaaattccat acctgttcac cgacgacggc gctgacgcga tcaaagacgc ggtgatacat
    24721 atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcagccc
    24781 ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt tctcctgcca
    24841 ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc tttggacata
    24901 ccaaccgtaa taacggttaa ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg
    24961 agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt
    25021 tgcttccgcc agtggcgcga aatattctcg tgcaccttgc ggacgggtat ccggttcgtt
    25081 ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca gctctttaat
    25141 cgcctgtaag tgcgcctgct gagtttcccc gttgactgcc tcttcgctgt acagttcttt
    25201 cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagcagcagt
    25261 ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt cagcgtaagg
    25321 gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt ggtcgtgcac
    25381 catcagcacg ttatcgaatc ctttgccacg caagtccgca tcttcatgac gaccaaagcc
    25441 agtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg ccactgaccg
    25501 gatgccgacg cgaagcgggt agatatcaca ctctgtctgg cttttggctg tgacgcacag
    25561 ttcatagaga taaccttcac ccggttgcca gaggtgcgga ttcaccactt gcaaagtccc
    25621 gctagtgcct tgtccagttg caaccacctg ttgatccgca tcacgcagtt caacgctgac
    25681 atcaccattg gccaccacct gccagtcaac agacgcgtgg ttacagtctt gcgcgacatg
    25741 cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg gtgtagagca ttacgctgcg
    25801 atggatcccg gcatagttaa agaaatcatg gaagtaagac tgctttttct tgccgttttc
    25861 gtcggtaatc accattcccg gcgggatagt ctgccagttc agttcgttgt tcacacaaac
    25921 ggtgatacgt acacttttcc cggcaataac atacggcgtg acatcggctt caaatggcgt
    25981 atagccgccc tgatgctcca tcacttcctg attattgacc cacactttgc cgtaatgagt
    26041 gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa cctttcggta taaagacttc
    26101 gcgctgatac cagacgttgc ccgcataatt acgaatatct gcatcggcga actgatcgtt
    26161 aaaactgcct ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc accaacgctg
    26221 atcaattcca cagttttcgc gatccagact gaatgcccac aggccgtcga gttttttgat
    26281 ttcacgggtt ggggtttcta caggacgtaa cataagggac tcctcattaa gataaaaata
    26341 ctggtcaacc tttaatctga ttagttaacc aattctgtgt tgcacacagt tatctgtgag
    26401 aaacccatgg aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa aaaccaaaaa
    26461 ctgcaatacc cacagcgtga tattgatcgc attaatcttt aaaacggcgt tgacagaagc
    26521 cataaaataa gcaaccgaac gttcgttcga gtatattggg aacgataaac tctcatgaca
    26581 tatggctacg gtgaatattt ttggggggga attctccgat cccggctaga ttgtattttg
    26641 tacagggaaa ttaaagtatg aaacgcgggc acagttatca ccagcccgcg aaatgagagg
    26701 atcaggatgc ggttaagata ccgccaatca tcgcgcgtaa tccctgagca aacgacgctt
    26761 cacgcgccag atcttccgct tcgatatcgg ccagtacacc gtaggtcaga tccagcagta
    26821 aacgtgaagt cattgacgtg ttgatgtctg ggcttatctc gcctttttgc tgggcatcac
    26881 gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt atttttaagc atggcggcga
    26941 cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt tgcccaaagg
    27001 cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga atcccttcaa
    27061 tcggttcccg gaaacgggcc agcgccctct cctggtcctg taagataatc gcctgaatca
    27121 aggcttcttt ggagatgaaa tggtgataga gcgtcccggg actaatggcg caggatttac
    27181 agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct ctggcagcat
    27241 tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc attaaaccat
    27301 tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat cattgctgtt
    27361 gaatagtgcg aaggcacact ctattcgtat gcttttcatc cagctattgg ttaaccaata
    27421 gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca cgctatcccg
    27481 gtaaacaaca catacggctg tttaccggtt cgttagtgta ttaattgagc tcctgtaccc
    27541 caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc gagcaaagga
    27601 acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta
    27661 acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt aatattgccc
    27721 ccggcgcaat gccattcacc cgaatatttt tttcacccag gtcaaatgcc atatttctga
    27781 ccagatgact ggccgcagct ttagatgatg cataggaagt catgtttata tttttatttt
    27841 ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc atttctggcg
    27901 caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca cggcgaaaat
    27961 ctgccattgg catatcaaac ggtttaggac caccgccacc ggcgttgtta accaggatat
    28021 caactttacc cagcttactg acggcaaagt ctgccagtgc agagagttcc tgttcggaag
    28081 taatatcaca acggcaggca aatgcctgac cacccagttg ttgaatttcg tctacaacat
    28141 ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct gtcgcgaatg
    28201 taatggcgat ttctttacca atacctgcac ccgcacctgt gatgatggcg cattttccgt
    28261 cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag taaaaataaa
    28321 aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggac actaccaggt
    28381 atagcagata aagagaggat tatttcgccg caatgagccg ggcgggaata ataagattgc
    28441 gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt gtggtaccaa
    28501 gttcccgcgc aggcgtgctg gcccaggtaa cgggtatatc atcaagtgtg gttggtgtcg
    28561 catcggtaaa tgccgccagc gaaacttgtt gctcaaagta acgatcgact ccgctttccc
    28621 cgctttgccg ccctgctttg agcaaaccaa accatgcccc catcgcaata gtttcgttat
    28681 agcaaaccac agcactgatg gtcgggttat gacgtaaaag cgccgtgata gcttccgcgg
    28741 cttgcttctg gctggatgtg cactccagca cccaatcgct gtgaaacggc aggccaaatt
    28801 ttagtagagt tgcacaatag ccccccaccc gttctgcacg ggttaatgag gaactttgcc
    28861 ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag atgctccgtc aacaactgtg
    28921 cagcctgcat gttgtccggg cgaaccgtat caacatcatc aagataactg gcacgggaag
    28981 cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg ctgcttcctg
    29041 cagccccggc aattaccaca ccatccacac cctgattcag taacagtgaa aaccgctgtg
    29101 ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc tgcgcttcca
    29161 gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg cgcagataaa tcacggacga
    29221 tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctggcga ttgcgcacaa
    29281 atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag attcgccctt
    29341 tgccactaag caccagcgaa acggtactta ccgacacgcc cgcagccagc gcaacatcat
    29401 gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt atgtgacaga
    29461 taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta atgtgattta
    29521 tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg aatccagatt
    29581 gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca cgctgtggga
    29641 gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt cgttctgcgg
    29701 cattatgctc ggcattggta gttctcttag cagccatgat gtcataaccc tgatcccggt
    29761 cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg gctcgtttgc
    29821 ttttagtttc ctgcctgtca tgttctgtat cgccatcccg ctgggcctgg cacgcgaaaa
    29881 taaaggcgta gcggcattcg ctggcttcgt cggttatgcg gtaatgaacc tcgcggtaaa
    29941 cttctggttg accaataaag gcattctgcc aaccacggat gccgcggttc tgaaagccaa
    30001 taacatccag agcattcttg ggatccagtc gatcgatacc gggatcctcg gtgcggtgat
    30061 cgccggtatt atcgtctgga tgctgcatga gcgtttccat aatatccgcc tgccggatgc
    30121 gctggcattc ttcggcggta cgcgcttcgt accaattatc tcctcgctgg tgatgggcct
    30181 tatcggcctg gtgattccat tagtctggcc gattttcgcc atgggtatta gcggcttggg
    30241 ccatatgata aacagcgcgg gtgatttcgg accgatgctg tttggtaccg gtgaacgtct
    30301 gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta ccgacgcagg
    30361 cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct tccaggcgca
    30421 attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc tttcgcaagg
    30481 taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg ttagctatgt atcactgcgc
    30541 gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga tcgcctgtgt
    30601 cgttggcggc acgaccgaac cgctggaatt cctgttcctg ttcgtggcgc cagttctgta
    30661 tgtcatccac gcgctgttaa ccggcctcgg cttcactgtc atgtctgtgc tcggcgtcac
    30721 catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt tgcatggtct
    30781 gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg tttactacgt
    30841 catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg atagcgaagt
    30901 tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt acaacgttcc
    30961 tgcaatcctc gaagcattag gcggtgccga caatattgtc agcctcgata actgcattac
    31021 ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac tgaaggacaa
    31081 tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta tcgggccaca
    31141 agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccggg cataaggata
    31201 agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta cacagtggga
    31261 ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt cagacatgga
    31321 ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc acggcgtatt
    31381 tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact ggttttccac
    31441 ccagcattac accgcaattg atacgcagac ggtggtgtat ggcccttctg tcatctatat
    31501 ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga tccacacgcc
    31561 cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa tgcccgttgc
    31621 tttagagaag caggctgacg actggttttg cgatatgggc aagctggaag ccgtgttggc
    31681 gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg ggaaagtgtg
    31741 gacgtgcgat gagctggaga tcatggctga cctgtgcgag cgtcatggtg tgcgggttat
    31801 ttccgatgaa atccatatgg atatggtctg gggcgagcag ccgcatattc cctggagtaa
    31861 tgtggcgcgg cgagactggg cgttgctgac gtcgggctcg aaaagtttca atattcccgc
    31921 gctgaccggt gcttacggga ttatagaaaa tagcagcagc cgtgatgcct atttatcggc
    31981 actgaaagga cgtgatgggc tttcttcccc ttcggtactg gcgttaactg cccatatcgc
    32041 cgcctatcag caaggtgcac cgtggctgga tgccttacgc gtctatctga aagataacct
    32101 gacgtatatc tcagataaaa tgaacgccgc gtttcctgaa ctcaactggc agatcccgca
    32161 atccacttat ctggcctggc ttgatctacg tccgttgaat attgacgaca acgcgttgca
    32221 aaaagcgctt atcgagcaag aaaaagtcgc gatcatgccg gggtatacct atggtgaaga
    32281 aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt tcgaaactgg aaaaaggtgt
    32341 ggctggatta ataaacgcca tccgcgctgt tcgctaaccc caattgcgca acgtaaaaaa
    32401 tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg cacttttata
    32461 ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat atccatcgcc
    32521 accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag tataatatct
    32581 cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt gccaacgaac
    32641 ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc gcctctcttg
    32701 atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac ggcctgcact
    32761 atgtcgagct gcgtttttca ccaggctaca tggcaatggc acatcagctg cctgtagcgg
    32821 gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg
    32881 cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag caagagctgg
    32941 aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt gatgaacttg
    33001 gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata
    33061 ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg attcgtgaac
    33121 tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggcg ctgatggatt
    33181 ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt cagaccagct
    33241 ccgtagcaga gctggctgca catccgctga aaacgttcct tgagcatggc attcgtgcca
    33301 gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa tataccgttg
    33361 ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca ggcacagatt aatggtctgg
    33421 aaatggcttt cctcagcgct gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt
    33481 aacaaaatgg atggtgcaaa ttgcaccatc catttttcat gcaaggcaca aagtcgcgcg
    33541 atgtttggcg gattcgatgc ccagctcaat caactccatt acctggattg cctggcttgc
    33601 cggaaccgga ttttcaccat cgccatttaa cgcatcacga atagccgcat agtaagccgg
    33661 ataattccca ggcaccgtca acagcgtttc ttcgacacgt tcctcacctt ccacgcgggt
    33721 aagtacgcca tcacgcatat cgtagcccca gtcttcctgc ggtagacgct cgccattttt
    33781 cagacgttct tcctgtggat cgaggccata tttcacataa ctgcctcggg atccatgcac
    33841 gatataccgt gctgactcag cggctgccag catggtaccg tgtaaaatga ctcgccgctg
    33901 tggataggac aagatggcgt ggaaataatc ggtcgactgc gctccgggcc gtaactgtgc
    33961 caaatctacc gtcatgctga ccggtaaacc aaaaagcgta atggcctgat caagaagatg
    34021 tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc tgttcacgcc aacgatcgcg
    34081 tacctgcgga cggaagcggt caaaatgaga ctcaaagtaa gcaacttcac ccagcacgcc
    34141 ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc cagcgacggt tatggaatac
    34201 agacagcaca cgccccaggc tttttgccag cgcatccagc tctcgcgctt gtgacagtgt
    34261 cacggtaaag ggtttatcaa cgaccacatg tttacccgcc tcaagcgccg ctttggctaa
    34321 cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg tctatgttgg gatcgttaaa
    34381 cagatgcttc ggctcagaga caaccgtaac cgttggccag tcggctttta cttttgtttc
    34441 atcactgctg gagattaccg ccagttccag cccgggcgtg cccgcaatca ggggcgcatg
    34501 gaaggttttg ctcgcataac catacccaat caacccaaca cggatgttgt cgctcatgtc
    34561 attgcctctc attgaagtat gatggctatt tgacactatc ctttacccac gctcaacagt
    34621 ttaataacct gccagcaata agggatgttg tttaacttaa gtcaaaaaaa tagcgaattt
    34681 tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa aatcttgctg gagctattat
    34741 tgctaagtaa catttacccc ctgaagttaa tggatcaatc aagagagatg tgggctgtaa
    34801 tgaatcgtct tattgaatta acaggttgga tcgttcttgt cgtttcagtc attcttcttg
    34861 gcgtggcgag tcacattgac aactatcagc cacctgaaca gagtgcttcg gtacaacaca
    34921 agtaagctct gcacttgtgg agcgacatgc tgcccgtccg ggtgcatgtt ttcacttgtc
    34981 ggatattaaa ccaggaattt attatcttgt ttgatgttgt tggtgattgt cagggatagt
    35041 aaagcgcgtt actcttttca gagcagtttt tcatccctga atatttgcca tcagcgcatt
    35101 cttattattg taataacaca agcgttcact tttggtgaaa ttttatcatc actaataata
    35161 cctacccctt tggtattaat tattttatgg accttttatg actgttcagg actacttatt
    35221 aaaatttcgc aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta
    35281 caccctgacg gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc
    35341 agagctggtt tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca
    35401 ctatgtccaa taaagaaagt agcgatcggt tataaaagcc ttataataac gcccctaaaa
    35461 ttagatttgc ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac
    35521 gtattggagg ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga
    35581 cgctggcgtt gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg
    35641 gcggacaagc actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca
    35701 tgtggtatta cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta
    35761 aacactatat tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt
    35821 cacccgtgga agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat
    35881 tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat
    35941 aaccttacag ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt
    36001 atttcaggcc gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat
    36061 aactttgtac tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg
    36121 gaaaccgcga tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc
    36181 gcctggctta tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg
    36241 gcatttattc tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc
    36301 agcccggtgc tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca
    36361 gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg
    36421 tacggttttt ccgccgctat cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc
    36481 gaacgccttg ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt
    36541 accgcaggtc ttatgtctct ggcctttatg ggctttagtg gtttggtgaa gttgtaatga
    36601 atgctatctg gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc
    36661 tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg
    36721 aaatcttacc gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg
    36781 aagccatcag ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga
    36841 tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag
    36901 agctaacgcc tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta
    36961 aatgtattca ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg
    37021 taatgagtga tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca
    37081 tctcgttgca accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc
    37141 ccgtgcgtat cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat
    37201 aaaatctggg atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt
    37261 acacccctgc gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt
    37321 ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt
    37381 acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct
    37441 attgcgcccc actctacggc tcatccttca gctttagctg aattaagcgt gattattgat
    37501 gccgatggtg aagactgctg gatcccacga gacggctggg ccgattatcg ctctcgccgt
    37561 cgcgaagagt taatcgagcg cattcatcag tttggtgttg ccgggctggg cggtgcggga
    37621 ttcccgacag gcgttaaatt gcagggcggc ggagataaga ttgagacgtt gattatcaac
    37681 gctgctgagt gcgagccgta cattaccgcc gatgaccgtt taatgcagga ttgcgcggct
    37741 caggtcgtag agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc
    37801 ggcattgaag ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct
    37861 cacgatattt ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta
    37921 acctacattc tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta
    37981 ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg
    38041 attaccgagc gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg
    38101 gcacggctgg ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc
    38161 gatcaaatgg tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc
    38221 ccggtcgtaa agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca
    38281 caggaagaac aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt
    38341 ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat
    38401 aacattgctg attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc
    38461 ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag
    38521 cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa
    38581 gcggctcgcc ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat
    38641 gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt
    38701 gtgattaaag cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt
    38761 aaagcgcaag ccagagcgat acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt
    38821 gctgatccac gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg
    38881 gaacagcaac aggctaatgc ggaaccagaa gaacaggtcg atccgcgca
//
LOCUS       DAMZII010000031        38654 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZII010000031 DAMZII010000000
VERSION     DAMZII010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.031"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            90..2333
                     /gene="pdeF"
                     /locus_tag="POA77_001563"
     CDS             90..2333
                     /gene="pdeF"
                     /locus_tag="POA77_001563"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDI6365023.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMIWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2372..3913)
                     /gene="ppx"
                     /locus_tag="POA77_001564"
     CDS             complement(2372..3913)
                     /gene="ppx"
                     /locus_tag="POA77_001564"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDI6365024.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3918..5984)
                     /gene="ppk1"
                     /locus_tag="POA77_001565"
     CDS             complement(3918..5984)
                     /gene="ppk1"
                     /locus_tag="POA77_001565"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDI6365025.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6155..6793)
                     /gene="purN"
                     /locus_tag="POA77_001566"
     CDS             complement(6155..6793)
                     /gene="purN"
                     /locus_tag="POA77_001566"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI6365026.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6793..7830)
                     /gene="purM"
                     /locus_tag="POA77_001567"
     CDS             complement(6793..7830)
                     /gene="purM"
                     /locus_tag="POA77_001567"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI6365027.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8155..8781
                     /gene="upp"
                     /locus_tag="POA77_001568"
     CDS             8155..8781
                     /gene="upp"
                     /locus_tag="POA77_001568"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI6365028.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8867..10156
                     /gene="uraA"
                     /locus_tag="POA77_001569"
     CDS             8867..10156
                     /gene="uraA"
                     /locus_tag="POA77_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI6365029.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTMI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10206..10952
                     /gene="hda"
                     /locus_tag="POA77_001570"
     CDS             10206..10952
                     /gene="hda"
                     /locus_tag="POA77_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDI6365030.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11090..11449)
                     /gene="arsC"
                     /locus_tag="POA77_001571"
     CDS             complement(11090..11449)
                     /gene="arsC"
                     /locus_tag="POA77_001571"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDI6365031.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11470..12933)
                     /gene="bepA"
                     /locus_tag="POA77_001572"
     CDS             complement(11470..12933)
                     /gene="bepA"
                     /locus_tag="POA77_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDI6365032.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13146..14207
                     /locus_tag="POA77_001573"
     CDS             13146..14207
                     /locus_tag="POA77_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6365033.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14245..15093)
                     /locus_tag="POA77_001574"
     CDS             complement(14245..15093)
                     /locus_tag="POA77_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDI6365034.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VIGWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15115..17127)
                     /gene="hyfR"
                     /locus_tag="POA77_001575"
     CDS             complement(15115..17127)
                     /gene="hyfR"
                     /locus_tag="POA77_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDI6365035.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17157..17570)
                     /gene="hyfJ"
                     /locus_tag="POA77_001576"
     CDS             complement(17157..17570)
                     /gene="hyfJ"
                     /locus_tag="POA77_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDI6365036.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMNFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17563..18320)
                     /gene="hyfI"
                     /locus_tag="POA77_001577"
                     /pseudo
     CDS             complement(17563..18320)
                     /gene="hyfI"
                     /locus_tag="POA77_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18317..18862)
                     /gene="hyfH"
                     /locus_tag="POA77_001578"
     CDS             complement(18317..18862)
                     /gene="hyfH"
                     /locus_tag="POA77_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDI6365037.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFEPTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAEQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18872..20587)
                     /gene="hyfG"
                     /locus_tag="POA77_001579"
     CDS             complement(18872..20587)
                     /gene="hyfG"
                     /locus_tag="POA77_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDI6365038.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRMMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20577..22163)
                     /gene="hyfF"
                     /locus_tag="POA77_001580"
     CDS             complement(20577..22163)
                     /gene="hyfF"
                     /locus_tag="POA77_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDI6365039.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLI
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(22168..22818)
                     /gene="hyfE"
                     /locus_tag="POA77_001581"
     CDS             complement(22168..22818)
                     /gene="hyfE"
                     /locus_tag="POA77_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDI6365040.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22830..24145)
                     /gene="hyfD"
                     /locus_tag="POA77_001582"
                     /pseudo
     CDS             complement(22830..24145)
                     /gene="hyfD"
                     /locus_tag="POA77_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24157..24267)
                     /locus_tag="POA77_001583"
     CDS             complement(24157..24267)
                     /locus_tag="POA77_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDI6365041.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(24284..25231)
                     /gene="hyfC"
                     /locus_tag="POA77_001584"
     CDS             complement(24284..25231)
                     /gene="hyfC"
                     /locus_tag="POA77_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDI6365042.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKMFKRQEIAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25242..27260)
                     /gene="hyfB"
                     /locus_tag="POA77_001585"
     CDS             complement(25242..27260)
                     /gene="hyfB"
                     /locus_tag="POA77_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDI6365043.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVAHFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFSGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27260..27877)
                     /gene="hyfA"
                     /locus_tag="POA77_001586"
     CDS             complement(27260..27877)
                     /gene="hyfA"
                     /locus_tag="POA77_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDI6365044.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28130..28600)
                     /gene="bcp"
                     /locus_tag="POA77_001587"
     CDS             complement(28130..28600)
                     /gene="bcp"
                     /locus_tag="POA77_001587"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDI6365045.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28600..29172)
                     /locus_tag="POA77_001588"
     CDS             complement(28600..29172)
                     /locus_tag="POA77_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDI6365046.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29318..30196
                     /gene="dapA"
                     /locus_tag="POA77_001589"
     CDS             29318..30196
                     /gene="dapA"
                     /locus_tag="POA77_001589"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI6365047.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30213..31247
                     /gene="bamC"
                     /locus_tag="POA77_001590"
     CDS             30213..31247
                     /gene="bamC"
                     /locus_tag="POA77_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDI6365048.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31460..32173
                     /gene="purC"
                     /locus_tag="POA77_001591"
     CDS             31460..32173
                     /gene="purC"
                     /locus_tag="POA77_001591"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI6365049.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32327..32554
                     /gene="vapB"
                     /locus_tag="POA77_001592"
     CDS             32327..32554
                     /gene="vapB"
                     /locus_tag="POA77_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDI6365050.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32554..32952
                     /gene="vapC"
                     /locus_tag="POA77_001593"
     CDS             32554..32952
                     /gene="vapC"
                     /locus_tag="POA77_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDI6365051.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33099..33962
                     /locus_tag="POA77_001594"
     CDS             33099..33962
                     /locus_tag="POA77_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDI6365052.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33977..35992
                     /gene="tmcA"
                     /locus_tag="POA77_001595"
     CDS             33977..35992
                     /gene="tmcA"
                     /locus_tag="POA77_001595"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDI6365053.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLQVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36066..36764
                     /gene="ypfH"
                     /locus_tag="POA77_001596"
     CDS             36066..36764
                     /gene="ypfH"
                     /locus_tag="POA77_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6365054.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36874..37074)
                     /locus_tag="POA77_001597"
     CDS             complement(36874..37074)
                     /locus_tag="POA77_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDI6365055.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37102..38229)
                     /gene="dapE"
                     /locus_tag="POA77_001598"
     CDS             complement(37102..38229)
                     /gene="dapE"
                     /locus_tag="POA77_001598"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDI6365056.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38233..38589)
                     /locus_tag="POA77_001599"
     CDS             complement(38233..38589)
                     /locus_tag="POA77_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDI6365057.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9413 a         9980 c        10163 g         9098 t
ORIGIN      
        1 atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat acacctcctt
       61 caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac
      121 gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc attttcgccc
      181 acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctttgg
      241 cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg atcaccttag
      301 ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca ttaacggcta
      361 attttattat cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta
      421 acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg cagattgtgt
      481 ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga
      541 gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc aattatcagg
      601 ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt cgggtagtgc
      661 gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt gatgccaaag
      721 tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg ttgctgttat
      781 gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgc
      841 tgcccctgat gatatgggga gcgatgcgct atggttataa gctgatttcg ctgctctggg
      901 cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct
      961 ataccacgca gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt
     1021 atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag cggcttgcgt
     1081 atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg ttgcgtgatg
     1141 ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg gttaagaact
     1201 atggcatcat gctgcggatt caatacaagc aaaaactttc tcactggctg tcacccttgc
     1261 tggaaccggg tgaagatgtt tatcagcttt cgggtaacga tctcgcgctg cgactgaata
     1321 cagaatcgca ccaggagcgc attaccgcac tggatagcca tctcaagcaa tttcgtttct
     1381 tttgggatgg catgccgatg caaccgcaga ttggcgtcag ctactgctat gtgcgctcgc
     1441 cagtgaatca tatctacctg ctgctgggag agctaaatac ggtcgccgaa ctttccatcg
     1501 tgaccaacgc cccggaaaat atgcagcgtc gcggggcaat gtatttgcaa cgcgaattga
     1561 aagataaagt cgcgatgatg aatcgactac agcaggcgct ggaacacaac cattttttcc
     1621 tgatggccca gccgattacc ggtatgcgtg gtgatgttta ccatgaaatt cttctgcgca
     1681 tgaaaggtga gaatgatgaa ctgatcggcc ccgatagctt cttgccggtc gcgcacgaat
     1741 ttggtttatc gtcgagtatc gacatgtggg tcattgagca tacgctgcaa tttatggctg
     1801 aaaacagagc gaagatgccc gctcaccgtt ttgctattaa tctgtctcca acctcggtat
     1861 gtcaggctcg ttttcctgtt gaagtcagtc agttgctggc taaatatcag attgaagcgt
     1921 ggcaacttat ttttgaagtc accgaaagta atgctctgac caatgttaag caggcgcaaa
     1981 tcaccttgca gcatcttcag gaattaggct gccagattgc gattgatgat ttcggcaccg
     2041 gctacgccag ctatgcacgg cttaaaaatg tgaatgccga tctgcttaaa attgacggca
     2101 gttttatccg caatattgtg tcaaatagtc tggattatca gatagtggca tcgatttgcc
     2161 acctggcgcg aatgaagaaa atgcgggtag tggcagagta cgttgaaaac gaagagatcc
     2221 gcgaggcggt gctctctttg gggatcgatt atatgcaggg ttatcttatt ggtaagccgc
     2281 agccgttaat tgatacgttg aatgaaatcg aacccattcg cgaaagtgcc tgaataatgc
     2341 gggccgacat ttcttgtcgg cccgcaaagc attaagcggc gatttcaggt gtactttctt
     2401 cttcaatttt caaccgccag ccagccacgc cttcccagta ttcttgctcc ttttccagat
     2461 caagcagtac cagcgcattc tgactaaacc agtcatgcgg gaaacgcagt gtccagtgac
     2521 tgtcatcagt aatcagtgtc aatgttggcg gtgtggtggt tgcctgacgt tgattgttta
     2581 gtaatacgcc aaggcgcaat agctgtatca gtggcaggaa ctgtttcttc ttaaacaagg
     2641 taaagcgcgg cagatcgtcg agcttaatcg ctttacggtg atagcgcacc agtgtcgcca
     2701 tcatcagctg ctgttcctga ttaaaacccg gcaagtcact gttttgcaga atataagcgg
     2761 agtggcgatg caaaccgctg tggttgatat tcaacccgac ctcatgcagc atggcggccc
     2821 atcgcagtag cgcctccagt tgcggatgcg ccagcttcgg ttgctgttcc cgccactgtt
     2881 cgtacatttg catagtggta tccagcaccc gtcgggcctg ctcgctgtcg atgtgatact
     2941 ggttggcgag gctgctggcg gtgcgactac gcacatcctg atgacggaaa cgtccttcca
     3001 tttcatacag tacgccttcg cgtaaagccc cgtcagaaag gcgcagttca cggatggcta
     3061 aagcatcaaa cacaccgcat aaaatcgcca gtccaggaac gaagactgtt ttccgctctt
     3121 cggaaagacc cggtaaactc agcgatgcga aattacggtg acgtaaaact tcttttacca
     3181 gtttttccag acgttccggg gtaattatcc cgtctttctc gcccatttcc attaacactt
     3241 catgggcggc ttttatggta ccggaagcgc ccattgccac gttccagccc tgaatacgga
     3301 attgccaggt taaagtttcc agtttttgtg ctgccgccat acgagcgcgc tgaaaattct
     3361 ctttattgat gaccccgcca gggaagtaaa gctgggcaaa gctgacacaa cccatccggc
     3421 ggctttcaac gagaataggt tcgaaatttt caccaatcac cagttccgta gatccgccgc
     3481 caatatcaat aaccagtttg cgacctttct ccggttgggt atgttccacg cccataaaaa
     3541 tcagacgggc ttcttcatta ccggaaataa tttcaatcgg gtagggaatg accttttccg
     3601 cgcgtttcag aaagtcagtg gcgttcagcg cctgacgcag cgtatgggtg ccgactatac
     3661 agacgctggc aggagaaaac ccttgtagcc gttcggcaaa cagcgacaga cagttcaaac
     3721 cgcgcgtcat tgcctcttca ctcaacatat tatctggccc caggccgtcc gccagatgca
     3781 cccgctgttt caggcggcca ataatctgca tggcaccatc taccacacgg gctatgacca
     3841 tgtgaaaact gtttgaacca agatcgaccg cagcaaactc ctgcggacga ggggatttat
     3901 cgtgtattgg catagggtta ttcaggttgt tcgagtgatt tgatgtagtc gtaaatcgcc
     3961 aactgcgccc gtactttgcg gcgattgccg cggggaacgt agcgattact gagttcttta
     4021 tcgatataac gtgctttgac cgtatcgctg aacaatatgt cgatgatgtc cagcacccgc
     4081 tgcttcaggc gcggatcgag cagcggtgtc gccacttcaa tacgataatc aatattgcgc
     4141 gtcatccagt cggcggaaga aaggtagacc tttttatcgc cgccattttc aaaaatataa
     4201 acccggtcat gttctaggta acggtcaaca atactgatgg cacgaatgtt gtcgctaatg
     4261 ccttccagat tggggatcag cgaacacatt ccgcgaacca gcagattaac cggtacgccg
     4321 gagctggagg ccgcatacag acgatcaacc aggcctttat cgacaaggtt atttagcttc
     4381 agggtgatac cgctgggcag cccttgctgc gcgttggcga tttcgcggtc taccatttca
     4441 tataacagac ggcgagagtt ttgcggtgac accattaaat aatcaaatgt caccgggcgg
     4501 tatgggtttt caataaagtt aaatacccgc cgtacttcgt tggtgatacg cgcatcggcg
     4561 gtcagcaatg aatagtcagt ataaagacgc gcggtttttt cgttaaagtt cccggtcccg
     4621 atgtgtgcgt aacgcaccac ttcaccgttt tcttttcgtg aaatcaggaa cagtttggcg
     4681 tgaattttca gccccggcgc agagaagata acgtgcacgc ctgcttcggt caggcgcttc
     4741 gcccagtgaa tgttagcttc ttcgtcgaaa cgcgcctgta actcaaccac cacggtgact
     4801 ttcttaccgt tatgtgcggc gtggatcatc gagtcgatga tgcgtgaatc tttcgccacg
     4861 cggtaaatat taattttaat cgccagtacg ctcgggtcga acgaagcctg acgcagcagt
     4921 tccagcacat gctcaaaggt gtgataagga taatagagca acacatcgcg ttcgcgaatg
     4981 gcatcaaaac cattgcggaa ctgggcttta tcaaaccaaa tatggcgtaa acgcggcagt
     5041 ggtttgttca ccagattggc tttgccgaca ttggggaaat taataaagtc tttaaaatta
     5101 tgataacgac cgccggggac gatggagtcg tagcgggaaa tagtcagttt ttcgcgtaac
     5161 acttcaacca gcgcattggg catatcgcgc tgataaacaa aacgcaccgg ctcagcagtt
     5221 aaacgctgct tgagactgga agacatcaac tccatcaggc tggcttccat ctcatgcact
     5281 aaatcgtatt cggcatcgcg ggtcatcttc attgaatagg cattcagcgc gtcataatca
     5341 aagaagcctt tgaaaatatc atcaaggcag taacgcagaa tgttatccag aagaatcatc
     5401 ggcttgcgtc gacgcggcgc ttctggcggt aaattcacaa agcgcggcac tttatctgat
     5461 gggatctcca gcagcgcgta acggatggta tcgccacgga taatttccac cgccagatag
     5521 gtgtaatcat ctttcaggaa ctgcactaag tcagtgtcag gattgattaa aatcggcgta
     5581 atgtgctgac gcagatactg cttaaaataa tgacgcagcc agttttgttg attgacggag
     5641 agctggcgtt cattaatcag gaagatctgg ttacgcgcca tctccagcag cagctcgttg
     5701 tagaggccgt cgaattcctg atcggctttc agcacccggg actgaatttt gcccagtaaa
     5761 tggcgggaat gagagttgga gccttgttct tcgctaataa tgatgcgtcg cttcagttca
     5821 gcgaagcgga ctttatagaa ctcatcaagg ttattggaat agatccccag gaaacgcatc
     5881 ctttcaatca gcgggttaga tttgtccgcc gcttcctgaa gcacgcgttc attgaacgat
     5941 aaccaactga gctctttttc gatgtatagc ttttcctgac ccattaccac ttttactccg
     6001 ttttattcac gggacactgc ctggatatta tggcgagcaa tattggcaga tgtccaacta
     6061 tgccagaaaa gggggaaaaa aactgctgga gcttgcgatt tacaggtgaa ttgaaaaacg
     6121 ccagcggcag agctggcgct ttaactacgg ggcattactc gtcggcagcg tagccctgcg
     6181 gcggcagacg ttgaccatcc agccacgcgg cgttttcgtg cattttcaga cgaccatcgg
     6241 caaaccagct aatcaccagt ggataaatag cgtgttcctg ggtttgcacg cgggcggtga
     6301 tgtcatcttc cgtatcacca gcaaataccg ggactttcgc ctgtaaaata accgggccac
     6361 cgtccagttc atcggtgacg aaatgcaccg atgtaccgtg ctcttcatca ccattttcca
     6421 gcgcctgacg atgggtgtgt aatccgggat atttcggcag cagagaaggg tgaatgttca
     6481 gcaaacgccc ggcatagtgg gagacaaacg ccgggctgag aatgcgcata aaaccagcca
     6541 gcacgaccac atcgggtgcg tacatgtcga tttcatgaat caactcccgg tcataggctt
     6601 cacgactgtc aaacgcgctg gcgatgagcg tatgcgttgc aatacccgcc tggcgggcgc
     6661 gttcaaggcc gaacgcgtcg gccttattgc tgaaaactgc ccgtacggtg cctttaattt
     6721 tgttagtttt acaggcgtca ataattgcct gtaaattact tccgttgccg gaaataagca
     6781 ccacaatatt cattattcga taaccacgcg ttgttcggaa tcagaggctt tgatgatacc
     6841 gattttccac gcgttttcac cgttggagtt tagcagggcg agggctttgt ccacttccgg
     6901 agcaggcagg gcgataatca tcccgacgcc gcagttgaag gtgcgataca tttcatggcg
     6961 ctcaacgtta cctgccgttt gcagccagtt gaacacttcc ggccactgcc aggaagattc
     7021 atcaatcact gcctgagtat tatctggcaa tacgcgtgga atgttttccc agaagccgcc
     7081 gccggtcagg tgcgcaatgg catgcacatc gaccttttca atcaactcca gcactgactt
     7141 cacgtaaatg cgggtcggtg ccagcagatg atcggctaat ggcttaccat caagttcggt
     7201 ggtttgcgga tcacaaccgc tgacttcaag aattttgcgc accagcgaat agccgttcga
     7261 gtgcggaccg ctggaaccga gtgcaatcag cacatcgccg tcgctgactt tagagccgtc
     7321 gatgatttct gatttttcta ccacgccaac gcagaaaccc gcgacatcgt aatcctcacc
     7381 gtgatacatc cccggcattt ctgccgtttc gccacccacc agtgaacagc ccgattgcag
     7441 acatccttcc gcgataccgc tgatcaccgc tgaagcggta tcaacatcca gttttccggt
     7501 tgcgtaatag tcgaggaaaa acagcggctc tgcaccttgc accaccaggt cattaacgca
     7561 catggcgacc agatcaatac caatggtgtc gtgacgtttt aagtccatcg ccagacgcag
     7621 cttggtacct acgccgtcag taccggaaac cagcacaggt tcacgatatt tttgcggcaa
     7681 tgcacacagc gcaccgaagc cgcccagacc gcccatcact tccggacgac gcgttttctt
     7741 cactacgcct ttgattcttc caaccagagc attacccgcg tcaatatcaa caccggcatc
     7801 tttgtagcta agagaggttt tatcggtcac tgcttgggtc cccacgcgtt acttgcggta
     7861 gaaaaataaa attcggcgca attctaacag ggaaagcaaa cgtttgcgag actgctttac
     7921 acaacctttt tgcacgtctt ttgcccaggc acgcggcgaa agaagacttg tgccagggta
     7981 aaggttagtt ttcggatgga ataatcttct ttcataacca tttgaatata aaataacttt
     8041 atctcaaacc gttatcattt tgactaaagt caacgaaaag aatattgccg ccttgaagaa
     8101 aggaggtata atccgtcgat tttttttgtg gctgcccctc aaaggagaaa gagtatgaag
     8161 atcgtggaag tcaaacaccc actcgtcaaa cacaagctgg gactgatgcg tgagcaagat
     8221 atcagcacca agcgctttcg cgaactcgct tccgaagtgg gtagcctgct gacttacgaa
     8281 gcgaccgccg acctcgaaac ggaaaaagta actatcgaag gctggaacgg cccggtagaa
     8341 atcgaccaga tcaaaggtaa gaaaattacc gttgtgccaa ttctgcgtgc gggtcttggt
     8401 atgatggacg gtgtgctgga aaacgttccg agcgcgcgca tcagcgttgt cggtatgtac
     8461 cgtaatgaag aaacgctgga gccggtaccg tacttccaga aactggtttc taacatcgat
     8521 gagcgtatgg cgctgatcgt tgacccaatg ctggcaaccg gtggttccgt tatcgcgacc
     8581 atcgacctgc tgaaaaaagc gggctgcagc agcatcaaag ttctggtgct ggtcgctgcg
     8641 ccagaaggta tcgctgcgct ggaaaaagcg cacccggacg tcgaactgta taccgcatcg
     8701 attgatcagg gactgaacga gcacggatac attattccgg gcctcggcga tgccggtgac
     8761 aaaatctttg gtacgaaata aagaataaaa ataattaaag ccgactttaa gagtcggctt
     8821 ttttttgagt aaagcgccta taacacataa tacagaggat aatactatga cgcgccgtgc
     8881 tatcggggtg agtgaaagac cgccactttt acagacaatc ccgcttagtt tgcaacattt
     8941 gttcgccatg tttggtgcaa ccgtcctggt gcccgtctta tttcatatta acccggcgac
     9001 tgtactgtta tttaacggta ttggaacgct gctgtatctc ttcatctgta aagggaaaat
     9061 tccggcttat cttggttcca gctttgcctt tatttcaccg gtattgttac tgttgccgtt
     9121 agggtatgaa gtcgcgctgg gcggctttat tatgtgcggc gtgctgttct gcctggtttc
     9181 ttttatcgtg aagaaagcgg ggaccggctg gctggacgtg ctgtttccac ctgcggcaat
     9241 gggcgcaatc gttgccgtca tcggtctgga gctggcgggc gtagctgccg gtatggcggg
     9301 tttactcccg gctgaagggc aaacgccaga ctccaaaacc atcatcatct ctattaccac
     9361 cctggcggtc acggttttag gttccgtgct gtttcgtggt ttcctggcaa ttatcccgat
     9421 tttaattggc gtgctggtgg gctacgcgct ctctttcgca atgggaattg tcgataccac
     9481 gccgattatt aatgctcact ggtttgcgct gccaaccctc tatacgccgc gcttcgagtg
     9541 gtttgccatt ctgactattc tgccagcggc gttagtggtt attgccgaac acgtagggca
     9601 cctggtagta acggctaata tcgtcaaaaa agatctgctg cgcgatccag gtctgcaccg
     9661 ttcgatgttt gctaacggcc tgtcgaccat gatctccggc ttctttggct ctacgccaaa
     9721 taccacttac ggagaaaaca ttggcgtgat ggcgatcacc cgtgtttaca gtacctgggt
     9781 tatcggtggg gcggcgattt tcgctatcct gctttcctgc gtcggtaaac tggctgccgc
     9841 tatccagatg atcccattgc cggtgatggg cggcgtttcg ctgctgcttt atggtgtcat
     9901 cggtgcttcc ggtattcgtg ttttgatcga atcgaaagtg gattacaaca aagcacagaa
     9961 cctgatcctg acttccgtga ttttgatcat cggcgtcagt ggcgcgaagg taaacatcgg
    10021 cgcggcggag ttaaaaggta tggcgctggc gaccatcgtc ggtatcggct taagtttgat
    10081 tttcaaactg atcagtgtgc tgcgccctga agaagtggtg ctggacgcag aagacgcgga
    10141 tataacagac aaataatgta ccgaccgggc agtgttgctg cccggttcaa acatcatggg
    10201 attctgtggt aaacttctcg cgattttgtg aaatcctggt tgaggtatct ctgaacacac
    10261 cggcacagct ctctttgcca ctttatcttc ctgacgacga aacctttgca agtttctggc
    10321 cgggggataa ctcctcttta ctggccgcgc tgcaaaacgt gctgcgtcag gaacatagcg
    10381 gttacatcta tctctgggca cgcgaaggcg cggggcgcag ccatctgctg cacgcggctt
    10441 gcgcggaatt gtcgcagcgt ggcgatgcgg tgggctatgt cccgctggat aaacgcacct
    10501 ggtttgttcc ggaagtgctc gacggtatgg agcatttgtc gctggtctgt atcgacaaca
    10561 ttgagtgtat tgcaggcgat gagttgtggg agatggcgat tttcgatctc tacaatcgaa
    10621 ttctggaatc gggcaaaaca cggttgttga tcaccggcga tcgtccaccg cggcagttga
    10681 atctgggatt accggatctc gcgtcgcgac tcgactgggg gcagatctac aaattgcagc
    10741 cactttctga tgaagataag ttgcaggcgc tacagttacg cgcgcgtttg cgtggttttg
    10801 aactgccgga agatgtgggg cgtttcttgc tgaagcggct cgacagagaa atgcgcacgc
    10861 tatttatgac gttggatcag ttggatcgtg cgtcgattac cgcgcaacgt aagctgacca
    10921 ttccgtttgt gaaagaaatt ctgaagttgt agataaggtg tttattgtcg gatgcggcgc
    10981 gaacgcctta tccgaactac gcgaagttaa tccatagtat tcgtaggccg gataagacgt
    11041 ggcaagcgtc gcatccggca atgtgcttca actagcgatg gagcacatct taacccacga
    11101 tttccagtac ctgctccggt ggacgaccaa tccgcgcttt gccattcgcg accacaatcg
    11161 ggcgctccat cagcttcgga ttatccacca ttgcctgaat cagcgcctct tcgctaagcg
    11221 aactgtcagc caggttcagt tctttataaa gatcctcttt ctggcgcatc aattcacggg
    11281 cgctattcat ccccagcatt ttcagcaaat cgcgcagcgt tgccgcatca gcgggtgtct
    11341 caaggtagag caccacttct gactccacgc cgttttcttt cagcaggttc agcgtttccc
    11401 ggctctttga acagcgtggg ttatggtaga tttttacctg tttggtcatt gttcttcctt
    11461 taatgcgaat tacatcttgg tataaggctt aaagcgttcc tgcagctggc gcaactggtc
    11521 gatgcgcgca tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg aactggcgct
    11581 actcaacagc gaaatggcct gatcgagtcg tccggcgagc gcataacctt ctgctcgcgc
    11641 agccagctcc tgatcacggt tatttagcgc ggcttccgcc tgtgccagca aatcccagcc
    11701 gttgctgtca tctttattat taaaggtgta gcgattcaga atattggccg cttcttgtgg
    11761 ttgaccgcct tgcagataag cgttcgccag gttgagctgc aacaccggat tggtgcgtaa
    11821 atcgcgggca tttttcagac gattgatcgc ctcattggct ttgttttgcc caagatcgat
    11881 atcagtagcc agatcgagat accatgcgtt gccaggttct gccgccagta acggttgcag
    11941 cgttttacgc gcctcgtcgt atttattggc ttccatcgcc tgtaaagcac gaccatattg
    12001 cgccgctcgt tgctgacgaa cgtttccttt cgcccattca tccagcaaat cactggtgag
    12061 ctggttacgt ccggaattat acatccccag tgtgcgcgct ttcgccagat agaaatcttc
    12121 cgacgactgc accaccatcg ggcgcatctg attagcacgg ttgcgggcat ctgccagacg
    12181 actttccggc aacgggtgag tcaataaaat ttccggcggg cgcgaggagt aacgcgcctg
    12241 atcgagtaat ttttcgagga aggttggcat cgcctgcgga tcgaatcccg agcgttgcag
    12301 cacctgaata ccaatgcggt ccgcttcctg ttcattttgc tgggtgaaac tgatcatccc
    12361 ctgacgcgtt cccgccagtg taccggtcag cgccgccatc cccgcctgcg gactggccat
    12421 cgccagtaaa atagaaccta acgcgccgac ccaggtcagc ggcgcgctgc gctgctgatc
    12481 ttccatcgct cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg ccataactga
    12541 agccagttga ctttcgttat cggaataacg gaacagggca gagtgcagca ccacgttgcc
    12601 gccaaagaaa gcaaaggcgt taatttcgtc gttgttgatc agaaaaaaat gaaacggtgt
    12661 cttaaccgaa ttggcatgcg aaaccagacg catccccagc gaattaatat attgcgttaa
    12721 cagcgggtca ttaattaacg gcgcgctgcc gcgtagctgg cggacataat agtcgcccat
    12781 ctgcatttcc tgaccaatgg aaagcgtgct tcctgcggag gttcccatat ccggcaaggt
    12841 gtctgcgctg tcggcaaacg ccggggctac ctgaccaatg gtcattgcag caatgagggt
    12901 tgcaaccagg ttttttttca actgcctgaa cataacctct atcctgtatt tctgaagata
    12961 gtcatttgac cgatactgtg aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg
    13021 aagattagcc cactcaaatg acttttcagt ttcctgaatc cgtcataaaa agcgaagtct
    13081 tttttgtgac atttcgatac aattgcggat cgcaatcccg ctcttgaggt tcagggaagg
    13141 tctttatgct cgaaatgttg atgcaatggt atcgccgccg ttttagcgac ccggaagcga
    13201 ttgccttgct ggttatttta gttgccggat ttggcattat ctttttcttt agtggcctgc
    13261 ttgctccgtt gctggtggct attgtgctgg cctatttgct ggaatggcca accgtgcgcc
    13321 tgcaatctat tggctgctcc cgccgctggg cgacgtcgat tgtattggtg gttttcgtcg
    13381 gtatattgct actgatggcg ttcgtggtac tgcctatcgc ctggcaacag ggcatctact
    13441 taatccgcga tatgccgggg atgctcaata agctttctga ctttgccgcc acgttgccgc
    13501 gccgctatcc ggcgttaatg gatgcgggca ttattgatgc aatggccgaa aatatgcgca
    13561 gtcggatgct gactatgggc gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt
    13621 tgctgaccat agccgtctat ctggtgctgg tgccattgat ggtcttcttc ctgctgaaag
    13681 acaaagagca gatgctgaac gccgttcgcc gggtgctgcc gcgtaaccgt ggactggcag
    13741 gacaggtgtg gaaggagatg aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg
    13801 agatgatcgt agtagggatc gccacctggc tggggttctt gctcttcggg ctgaactatt
    13861 cgctgctgct ggcggtgctg gtcggcttct cggttcttat tccgtacatt ggcgcatttg
    13921 tggtgaccat tccggtggtt ggcgtggcgc tattccagtt tggcgcaggt actgaattct
    13981 ggagctgctt cgcggtgtat ctgattattc aggcgctgga cggcaacctg ttagtaccgg
    14041 tgttgttctc cgaagcggtt aacctgcatc cgctggtgat tattttatca gtagtgatct
    14101 tcggtggttt gtggggattc tggggcgtat tcttcgccat tccattggcg acgctgatca
    14161 aagccgtgat ccacgcctgg cccgatgggc aaatcgcgca agaataatct tcagcccgac
    14221 gaactgtttc gccgggctga tttttcaggg ttcctgacgt aaataaatag cccgataaca
    14281 aatactcacc agcactgcac cgccgataat attacccagc atcaccggga gcagattggc
    14341 ggtaataaaa tggctgaccg tcagtgccgg aaaattgtct gcgctactgt gcgccagttg
    14401 ccagaaggag gtaggggcga aatggcgaat ggcaatggcg aacggaatca caaacaaatt
    14461 ggcgatacag tgctcaaagc cactggcgac aaacagggtg atgggcaaaa tcatggcgac
    14521 gattttgtcg cataacgaac gcccgcaata actcatccac agcgccaggc aaaccatcag
    14581 attgcacata atgccgaggc tgacagattc agtaaatgta tgatgcattt tgccctcggc
    14641 gcagtgtaaa accgcgactc cccacatcgc gttttcactc atcaccagcc cggaaaacca
    14701 gattaacaaa ctgaaacagg caatacctgc cagattaccg caggccacca gaagtgcgtt
    14761 aatcagccaa gttcgccaac caataacgcc ccgacttttt gccatcaccg tcattaccga
    14821 cgaggtgaac aggctggtgc cgcaaactgc cagcaaaata aagccgagtg taaagcaaag
    14881 gccgcccacc agatgggtta atgcctgtga agacggggca tctgcgataa cggaaaggta
    14941 aaaagtaaat ccgatcgcca taaacacgcc agcggacatc gccattaaaa agaccgacac
    15001 tttactgcgg gcaattttat gggcggcaat ccgttcagcg attgccgctt ttctggcgct
    15061 caactgcaag tcgaaagaga gtttgtttct catgcatact cctgcaaaag cagattacaa
    15121 cacctcgcga accgagatcc ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat
    15181 ccctaatcgt gtcgccgcgc cacggggccc ggcaacaatg ccattggttt ctcgcaatac
    15241 ctgaacaatg cgctgacgtt cttcttcgtc attctctggc gtcgtcgggt gcagcaactg
    15301 cgccattgaa ctgcgaagcg ctgaatcttc atttagcgtc ggcagtaaac ggctttgtcg
    15361 gacattaaga tgtaaattca gactgttacc gcgagtcagc agtaccgctc gctcaatcac
    15421 gttttccagc tcgcgcacgt tgcccggcca gtcccacgac atcaactggc gtagcgcctc
    15481 ggtcgggatg gcgtcaattg aacgattcat atggcgcgcc attttttgcg tgaaatgctt
    15541 tgctaaaaga gggatatctt ccggacggtc gcgtagcggc ggcaattcca gtgggaagac
    15601 attcaggcga taaaagagat cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc
    15661 acggttggtg gcggcaatga ctctgacatt caccgggatc gttctactcc cgccgagacg
    15721 ctcaatctcc cgttcctgca atacgcgcag cagtttaggc tgaagttcta acggcagatc
    15781 gccaatttca tcgagaaaca acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt
    15841 attaatcgca ccagtaaacg cccctttgtc atgaccgaat aactcacttt ccagaagact
    15901 ggcggggatg gcagcgcagt tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg
    15961 atggatcgct ctggcgatca cctctttgcc ggttccggtt tcaccgcaaa tcaacaccgt
    16021 actgtcgctc ttcgccacaa tatctacctg ctggagtagg tcttccattg cctggctttg
    16081 atagataatg tcaccgatgc ccagattcga aaggagctgc tcgctaagct ggtggttttc
    16141 ttgctgcaaa ctttcctgca aatcggtcat gctacgccag gcatcggcat tgccaacggc
    16201 aatagcgatg cgatcggcta tgtgttgtag tagctggcag ttttcctcac taaagagagt
    16261 ggaagaggta tgcgccagca acaatgcacc cggtgtatgg ttgccaaagg taagcggtat
    16321 aaggagcgca gattcgcagc cgttagatgc aagtaagagt aataacgggt cgcgttgcca
    16381 gagaaacaga tcgtctgcac ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt
    16441 cagcaataca ctgtcgccag gcatattgcg tggcagacac gcacaatgtg aggcagaaag
    16501 atcgctgcac cacaggctaa acttctcgtt ctttcgatga tcgcccagta ccatactgac
    16561 tgaagccaga ccgaaaaaat gatggatctc acgagcgacg tcagcgatca gatcgtcgag
    16621 atcaagatgt gacagcactg cattagtaat atcaaccagt acccgatgat gatcgcgatc
    16681 tttctccagc aaacgcagcg ccacctgctg ttcttcatca ttgagccatg attgcagaac
    16741 cggggtgatt atccgcgcca gccagttaaa cagtgtgcaa tcctggggag taaaacggct
    16801 gccgtgggta cgaataaagc gtagctccgc cattccacta ctgccgagaa ggacggcgtg
    16861 acgcgaataa tcgaccatct cgccctgata ctctgttgtt gccgtcacat cgctgccatc
    16921 atcatggtag cggtaataga agttgctttt gagaacatgc agttcaataa gttgtaccgg
    16981 gctgaacggc ggcggcatcg ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg
    17041 tgctaaccgc tccgcgagca ttccgtttac cgcttcaatt gttattcctt gtggcggggc
    17101 aaacatcgcc tcgtctgaca tagccatcaa cagtccttat taatgagaaa tccttctcac
    17161 gcaatttttc tggccatcag atagatggcc ggttcctgca caatttcatc caacagatga
    17221 atgaattgaa tgctacgcgc ctgccacaca gacgtcgcgt catccgccag tggcgcgaat
    17281 gcccgggtca acagggcggt gtggctggcg tcaataacaa tctcaccaaa attcatcacc
    17341 cccagcatct tacgtcgggc ttgctcctct tcgacctggg caagccaatc ttcgtattcc
    17401 gtcagtgggc aggggaaggc gacattcaga caatcaatca cgccaacgtg atgaccgata
    17461 gccagcgagt aatacattac ctgagagctg tgttccggca tctcgtcgct actggcgaca
    17521 aacttttttc gcagcgtcca gaaaacaatt tccccgcact cttcagtcat gtaacccccg
    17581 gagtacgcga aagagttgct gaacgatact attgagacgt ggatcgtcgg catcgcgcaa
    17641 ccaggtgtta acccgatttc ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca
    17701 aatttctcgc ccctgacgat agcccgccag ccgccgcgct tctcgctcaa tggcgatacg
    17761 ctgtgatggc gggatctgcg gccacaacgg ttgcatagtc accccggtgg gatcgcgata
    17821 atccaccgcg tgaatcttct gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt
    17881 ggcggccggt gttggcgggc agccggggat ccaaacatca atggggacaa tggtgtcgct
    17941 gccgccccag acgctgtaga gatcgtggaa aatccgccgc cggcaccgca cgcgccgtag
    18001 gaaacacaga ttttatgatc gggggcagac tcatacgccc gaagtgcagg catacgcatc
    18061 gcccgggtga ctgcgcctgt aaataacaaa atatcggcgt gacgcggtga tgaaataacc
    18121 ttaatgccaa aacgttctgc gtcgaatact ggtgtgatgg cggcaaaaat ttcgatttca
    18181 caggcgttgc agccgccgca atcgacgcga taaacgtaag ccgagcgacg gatatcctgt
    18241 agcaaatgct gcttcatctt ttgcgtttgc tcgtccagcg tgatgggctg gctgacatat
    18301 tgtgtaagca ctggactcat agctgctcct tagccaccag cggtacatct gtatcgtcgt
    18361 tgagcagcgt cgcgcgttgt ttgcactccg ggcagacgct cgcttgcgcc cataacattt
    18421 cgcggttttg tggcgcattt tgttgctctg ccaacaattc agcagccagt gcgacggttt
    18481 tttgctgggc aaacgggcgt tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg
    18541 tatagagatc ggctttattg gtgacggtcg gttcaaagtt attggtaagc tggatggctc
    18601 tggtcgggca cacttcttca caacgtccgc agtaaataca acgcctcaga tagagctgcc
    18661 aggtgcgcga attttgctgg tcgtcggtct ggatagtcag cgcatttgcc ggacaagcac
    18721 aggcgcaggc accgcaggca atacattggc tgggcatcag gtccggtttc ccgcgaaagc
    18781 ctgggctgac ctccagtggc gcgaagggat atttcaccgt cgcggttccg gcgcgcataa
    18841 tagttttcag taacttcagc atcttctgtc cttacttcag cggcgaacgg ttacgatcaa
    18901 tgccgtagcg ttcgatctct ttatacggca cggtttttga ctggcgcttg cgcacatcta
    18961 ccagcgtcac acggtcggta caggagtagc agggatcaag gctaccgata atcagcggtg
    19021 cgtcagaaac ggtattgccg cgcagcatgt aacgcaacac cggccagttg gcgtaggtgg
    19081 cggcacgaca acgccagcgg aacaattttt ggttatcacc gagcatgctc cagtgcacgt
    19141 cttcgccgcg tggcgcttca acaaagccca gcgcgaatgc gtgaggttta tagctaaagc
    19201 cttcggtcag cagtggggta tccggcatgt tgtcgagggc aaattccagc attgccagcg
    19261 aatcaaacgt ctctttgaca cggaccatca tgcgggagaa gacatcgccg ccggtaaagg
    19321 taaacagtgt ttttggaata ttgccgtagt cggcgtaggg gtgatcaaag cgcaaatcac
    19381 gggcaaaacc actgccgcgg atcagcggcc ctacagggct ataatcacgg gcgatttgtc
    19441 ggtcgagaat gccaatgccc tgagtgcgtt gttccatatt cggcgtggca agcagcatct
    19501 ctaccagctc cgacacgtcg gcgcgcatct cgcgcaccag tttcagcgtt tgcagacgtt
    19561 gctctttgag aatatcgcgg cgaacaccac caatcagatt cagaccgtag gttttacgcg
    19621 acccgatcag caattccgcc atcgtcatcg acttttcccg cacgcggaaa aattgcataa
    19681 agccggtatc aaaaccaacg aaatggcagg agaggccaag gttaagcaaa tgactgtgta
    19741 gccgttcgac ttccagcaga atcgagcgaa tagtatgtgc tcgttgcggc acctcaatcc
    19801 ccagtgcatt ttcaaccgaa ttggtatagg ccacactgtg ggcaaaaccg caaatcccac
    19861 acacgcggtc cgataagaag gtcacttcgt tgtagcccat ccgcgtttct gccagtttct
    19921 ccatgccgcg atggacataa aacaggcggt aatcagcatc gacaatttgc tcgccatcca
    19981 caaacaagcg gaagtgaccc ggttcatcgg aggtgatatg cagcgggccg acagggatca
    20041 cccgcgcatc gctgttgccc tcattgataa acgcatacgt ttcggaatct gtcgtcggtt
    20101 cagggcgcag tcgataatcc atcgcatctt tgcgcagcgg atgcatatct tccggccagt
    20161 catcgggcaa caccaggcga cgctgatccg gcaggccaac cggaatcagc ccgtacatat
    20221 cgcgaatttc tcgctcgccc cagaccgcgg cagggacgcg cggtgtgact gacggaaact
    20281 cccgactgtc ggcatcgacc agtgccttca ccacgatcca gcatttttcg gccccttcca
    20341 ttgaaagggc ataataaacc gcgtaatgac cgttaagtgt ccgctcgtcg ttgccaaaca
    20401 ggactggaag ccagccatca tgttgataat aaagataatg tacaacgtca ggcagcagat
    20461 tgattttcac cgtaatggtg acctgttcag gcgtttgtcg ctcttcatcc agcaccgcgc
    20521 cggggaactg cgttttcagg gcggcgagaa tcgcttcgcc acgatttgac gatgaattaa
    20581 cgttcactgt gtttctccga aacagatgcg gtgcctgaag gcaaaaaatc atgccaggtg
    20641 ctacgttgtg caggcaggtc gtgcgtccct gagaggacta tagtggaagc gcccgccagg
    20701 atcctgatga caggttgtgg aatatgcgtt cccatcgcca gcatcatgac cagcagaatc
    20761 accattggcg aggtggtcaa ccagccgaga tcaccccggt taacggcctg cggcggtttc
    20821 gccattaaca cccgcgctgc catccgtacc aggcccgcca gcaccagcgt taacagcaat
    20881 aacagcagga cgataatcag caggtgatta cgtgccagtc cggcggtaat ggtcataaat
    20941 tcgctaagaa aaatgttgaa gggcggcatc cctgccagcg ccagcgccag cgcaccgccg
    21001 ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc cacagacgac gttgagatcg
    21061 cgcgtgccgt acttgagcag tacattgccg gaaccgcaga acagcagcgt ttttgccaga
    21121 ctgtggttta agatgtgcag cagcgcggca aaaattccca gcggcccgcc aatgcctagc
    21181 gccaccgcga ccagccccat gttctccacg ctggagtacg ccagcagacg cttaatgtcc
    21241 cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa gatgagcaac
    21301 aaccggttgg ggaaatcgct gccgatggct tggcaaataa tgatgtaata gcgaatcagc
    21361 accaacagcg cgcagttcag caatacggcg gagagcaggg cgctgaccgg actcggcgct
    21421 tcactgtgag catccggcag ccaggcgtgc atgggaaata gcccggtttt ggtaccaaag
    21481 ccaattagca caaacacaaa ggccaacagt attaatgttg ggtcaagcaa ggacgattgc
    21541 ttaagaacct cgctccagaa tatcgccatt tctgcctgcg gcataacgct ggcggcgttg
    21601 gcgtatacca gcacggtacc gaacagacca aaagcgacac caacagtaca aataatgatg
    21661 tacttccatg ctgattccag cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag
    21721 cttaaggtgg tggcttcgat cgccgcccac atcacaatca ggttattgct ggtaacaacc
    21781 agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag tgtaacgggt
    21841 gaaagctcgc cgtgtgccac ttcatgacgc atgtagccaa tcgagtaaac accggtgaga
    21901 aagccaatca caccaagaat ggcgaggaac aaaccgccca gaccatcaat atgaagccac
    21961 agtcccgcag cgaatatttc tcctgcatca gcggcagttt ggaccaccca gagtgccaga
    22021 atcagcagca gtgtgatccc taagctatgt aatacagtca ccgtacaagt cgcagagagc
    22081 ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca gcggcgtgag caggagtaaa
    22141 gcgaacatca cagaataact catctcatta tcccttcagc gcggtcaagt tgttcacgtc
    22201 cagcgtgccg tgggtacgcc atatttttct tgccagtaac accatcacaa tgacggcgaa
    22261 gatggcgtcg gtagcgatac ctatttccac cagttccggt gctcgccagg caagaagcgc
    22321 cagcaccaga tgggagccgt tttccatcag gcagtaacca aaaatttgcc gcaggatatt
    22381 gcgctggctg acaatgcaca gcaggccaag cagaaaatga cctaacgcta ccgccagcgc
    22441 cggtttcagc ccggtagcca tcggtagctt cacgggctga acgacaaatg cgcaaagcag
    22501 gacaattaac gccgcgagca gtgccatcat tgccggaccg aataacgctt tttccgggat
    22561 gttctgggga atatttcgtg cagcgtaagt catgattaac ggtaccagca gcactttggt
    22621 gataaaggcg ctggcggacc agatcagcag ttgctctgcg gcgaacaggc acgagagagt
    22681 ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa aatccgcatg acaggcgata
    22741 gcttttgacg ctaatcacaa acagcgatgt cagcatcatc agtcccgcca gattatttac
    22801 gatcatagaa ccggtcattc atcatctcct tactgcaacc aggtcgcggc gattacgctg
    22861 gaaatcagcg acatcacaat cagtacaatc aacaccaggc gcattgaccc tggcagcggt
    22921 gcggcatcgg cgacggcctc gctcggtttg ccaggcacaa cgcgaccaaa ccagcgaata
    22981 aaccaggcga aactggcgac cgattcaatc atcagcagaa tcatggcggg cagcaggatc
    23041 cagtactcca ctgacaacgc aaaaccggca gcaaacagcg ggaatttact gaagaagccg
    23101 ttgaacggcg gcacgccggt aatcgccagc gctgccacgc agaaacccac gcctggcaac
    23161 ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc
    23221 agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg
    23281 atgctaccct ccagcgccag ccgcgagccg aagatggaga gcgacaagcc gaagaacatc
    23341 cagccaagtt gagtgatggt cgaccaggcc agcaaccgct tcatatcctg ctgtggcaaa
    23401 tacatcagaa agccataaag aatggtgacc agtgccatga ccatgccaac gccgccaatc
    23461 acatgcggga tattgccgcc gtcgataatt gcgcgggcaa aaatgtaaac gcccactttc
    23521 accatcgatg cggcgtggag ataggcgctg atcggtgttg gcgcttccat tgcatccggt
    23581 agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatcccgcca
    23641 taaaccagat aacgtgcgtc gccgtgtaac tcgctcattg cgctaagcgc aaacgttccg
    23701 gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgaaccgat atgggtgata
    23761 agcaacgctt ttagtgctga acgctgtgct ttatcgctct ggtaataact gatcaacgcc
    23821 caggagcagc cgccctaatt tcaaaaaaca acaactgacc gagcagcgtc gaggagagta
    23881 ccagtcccgc catcgcgccg ataaacacca gcaaaaatgc gtaataacga ttcgtgccgt
    23941 tatgcgggtg ttcgcgattt ttatccgtca gataacccgt ggagtagatc gtgaccagca
    24001 aaccgagaaa caccaccaca aacagaatca gcgtactcac gcgatcaatg accaggccaa
    24061 acaatgccac atcccccacg ttgaccaacg tcaacgtgac ggcaacttta tcggcctgat
    24121 aaaacgcgga gatcagcgac aacatgcaca gcgtggtcag tgcggcgaac aaaaccccca
    24181 ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa aaggcagcat
    24241 taacgtcgtc agagcaagat tttccatatt ccgtcagtgc tccttacaga ccggttaacc
    24301 agaagaccca tgcaagcgca gcaaggctga agccaagcca ggtcacatgg tgaatgagta
    24361 aaaaacgccc gcgtgccagc gtgttttctg cgattgaggc cagtacaaaa atcagcaaaa
    24421 ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc gagagaaagt tcttgcgcgc
    24481 gcccaaaggg cagaaacagg gccacaaaca gtgctgccat cacgacctgt ttcagcccca
    24541 gcccccattt cgctagcgcc agcccggcac cggaatattc ggtcagcggg ccttcctgta
    24601 attcctgttc tgcttcagca acatcaaagg gaatttttcc catctcaatg aagcaggcaa
    24661 aaccacaggc cagtaacgcc agtacggtgg ttagcggcga gttccagccc atcgccagcg
    24721 tattgctgat catctcgata tgcgtggaac ctgctatcag cgccaatacc agcagtgaga
    24781 gaataagcat tggttcgacc agaatgccga gcgtcaactc gcgactggca ccgactcccg
    24841 caaacggact tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac agggcaagaa
    24901 gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta ataaacagtg
    24961 gtaaggccat cgccagcacc agcatgctgc tgattaatac ccacggcatc aggcggaaca
    25021 tcagacctga agatgtcggc gcaatttcct ggcgtttaaa cattttgtgg atatcgcgat
    25081 aatcctgcca gatccccggc ccgcggcggg agtgcatacg cgcgcgtatc tgccgggaaa
    25141 tacccgtaaa aagtggggtt agcatcagca gaatcacggc ctgtgctaac gcaaaaataa
    25201 ccagatatcc gtcgcaaaga gtttgtctca tggtgatttc cttagacggc aatagcgatt
    25261 agcagcacaa ccagtgcggc gaccacatac aggcaataga gacgaaagtc gccgctttgc
    25321 agatgctgta tttctttggc cagccgttgg gtggcgctca cgatggggcg gatcacccgc
    25381 tcatcccaga agggttctgt gctctgagcc ctggcggtga cgtgcgcaaa acctttattt
    25441 agcctcagcg tagggtcgag ctgttgtcgt agacgaaata acgcagagaa gaccacacgc
    25501 agcggctgca tcacgccatt gcctgacggg gccatcgcat tttcccagcc gtagccgcat
    25561 gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca cagccagtaa
    25621 aggccaggca gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg ggtgacctgt
    25681 gtatgaaacg tgccgggtac aagtgctatt ccgctggcga cagtgacggg aggggtattg
    25741 gtaaacgcat gggcaatatg cattatcttc ggtgccagcc aactggcact aagcgcaatt
    25801 aatacgcaga gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg cacttcctgt
    25861 gcctcttcag cgtgtgtact gcgcggcgca ccactgaaag taataccgta catttttacg
    25921 aagcacatta ctgccagccc accagtgact gccagcatta ccatagcaat aggacccgca
    25981 agttgtagcg ctacggcttc cacacgactt agtgagaaca gcgactgcca ggtgtaccat
    26041 tcgctgataa aaccattcag cggaggaatg gctgatatcg cgaggcaacc aatcaggcat
    26101 gctgcggctg tccacggcat ccgtttcgcc agtgccccca ttttttccat gtcgtgggta
    26161 tgcaaacgcg aaataatcgc gcccgcgccg agaaatagca gccctttgaa cagcgcatgg
    26221 ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg gtcgtgcagt
    26281 gacagaccga ccatcgccac accgactgcc agcaaaataa tgccgacgtt ttcgacggta
    26341 ctccaggcca gcagccgttt gatatcctgt tccgccagcg catacagcac gcccaggagc
    26401 gcggagattg cgccgatcgc catcaccaga atgccccacc acagcggcaa acccgtttgc
    26461 gccagcaggt ccatcgctac tttcaggatg ccgaaaatac cgattttgac cattacgcca
    26521 gacatcaacg ccgaagcgtg cgatggtgcg gcagggtgag cgcgcggcaa ccagctgtgc
    26581 aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa caccgccgac
    26641 gccagccccg gagaaagtga aagcgtgcgg aaactggcaa aatcgaggct gccgctttcg
    26701 cgccccatca gcaagaaggc gatcataatc agcaccgatc cggcgtgggc gataaaaaag
    26761 tagagcatgc cagcgttgat cgacgtttta tcctgcctgg caatgaccag aaaccaggaa
    26821 gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat caccagcagg
    26881 gcaaccatcg atgcgatgaa aagattcata aagaagccca tcgccgccgc gcctttgccc
    26941 tcgtattcgc gcatataagt caatgaatag agcgaacaaa ccaccaccag caaggagata
    27001 accagcacca taaatgccga gaggctatcc attcgcaggg ttaaatcggc aaagtcaaaa
    27061 ggggtggcaa aatgggcgac taaagttaca ccagcatgta attgcgtaat tccgctgatg
    27121 atgccaatca cgccgcccac cagcgatgtg atgccggaaa gcttaatagc cagtctgtcc
    27181 agaccgagta aaaacagcga agccagactg gcaaacagat agaggatcag cgaccaggtt
    27241 aataattgca gggcatccat tagtgttgct cctgagtgag ggaaagggga tcctccccgc
    27301 cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt catctgacgt tgcaggctat
    27361 cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg aacgcaggcc ggaccttctg
    27421 gcaagaaatc acacaggtcg catttcaccg cgacggtctg gacacccggt tcccagcgca
    27481 gcaacgcatg ttggggcagc acgttttctt cgccgtcttt gagtgagcct tcagcctgaa
    27541 aaacatattg cgcatgggca ttcaccggac ggcttcctga agcgctgatt gcgccaaatg
    27601 ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct ttcgttgagt tggatcgcat
    27661 catccctctg agagatggca ttgaccgggc agacctgcag gcaaggggct tcctcacagt
    27721 gatgacacac gacaggggca gtgattgttg acgtcttcgc cagggccagg cgcgggtgtt
    27781 gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc gagacaggta ttacatcctg
    27841 tacaccacag tggttcggcc accacaaagc ggttcatgtc ggctccttga aaatgatatg
    27901 aaacattgca tttcatattg cataaaggat gccattctga ttaactcaat atattcaata
    27961 agttatgtat tgactggatt ctgaacggta caaaaatgat ggtcattaac gttcagcaaa
    28021 aaatggattc gacacccgtg tcgcagggga aagcgagaat gtgatcttga tcacgagaag
    28081 tgaggtatgc tggccgcaag cgcagccagc acggaatgga gcaaagtaat caggcgtgtt
    28141 ctttcagcca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg tcaaagacat
    28201 gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatgcca tcgtaggttt
    28261 tgcccatgaa ggacttttca ccccagacgc cgaattgttc gcacacctgg tggtcctcat
    28321 cagacaggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt ttttcgggtt
    28381 tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc atgttatcgc
    28441 gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag aaataaacca
    28501 gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga tccggcaagc
    28561 taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc catcctgttc
    28621 atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt ctgtacatag
    28681 ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt gggcggttat
    28741 ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt tgcgtgcgcg acaccagctc
    28801 cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga
    28861 gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg gacgcgccgt
    28921 cgtgcgcttc atcacgatta aaagatccag ttcggcacct ttcaacggta acgttgattc
    28981 aatcagagta atggcattcc atgaaccgga aagcagcata ataaacgtga actcttctcc
    29041 cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat gacgggtgat
    29101 ggtgttcaca attccagggc gatcggcacc caacgcagtg atcaccagat aatgttgcga
    29161 tgacagtgtc aaactggtta ttcctttaag gggtgagttg ttcttaagga aagcataaaa
    29221 aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc cataccaaac
    29281 gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa gtattgtcgc
    29341 gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat
    29401 tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca ctggcgagtc
    29461 cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg agctggctga
    29521 tgggcgcatt ccggtaattg ccgggaccgg cgctaacgct actgcggaag ccattagcct
    29581 gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc cttactacaa
    29641 tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgagc atactgacct
    29701 gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc cggaaacggt
    29761 gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag ggaacttaac
    29821 gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga gcggcgatga
    29881 tgcgagcgcg ctggacttca tgcaattggg cggtcatggg gttatttccg ttacggctaa
    29941 cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc attttgccga
    30001 ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg tcgaacccaa
    30061 tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg atacgctgcg
    30121 cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca
    30181 tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcaa aagtcgcgcc
    30241 tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt tctgactcac
    30301 gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg cttgcggagc
    30361 ttcatgcccc ggctggaatg attttgccgg tgacctccgg tgattatgca atcccggtga
    30421 ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc cagccgctgg
    30481 cactggtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg ctggtggaaa
    30541 atggtcgtgg caatactctg tggccgcagg tggttagcgt gctgcaggcg aaaaactaca
    30601 ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta caatggaacc
    30661 gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt
    30721 atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa ccggttgcag
    30781 acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc gccggtctgg
    30841 ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc actatggacg
    30901 tacaaagtgc agctgatgac accggtttac cgatgctggt cgtacgcggg ccgttcaatg
    30961 tggtttggca acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg accgacagca
    31021 cccgttcgca gggtaacatg gccgtaactt ataagccgct gtctgacagc gactggcagg
    31081 aactgggcgc gagcgatcca ggcctggcat ccggtgacta taaactgcag gttggcgatt
    31141 tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg actcagagtc
    31201 agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa tacagggctg
    31261 gaatcatccg gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacgc
    31321 gattttagcg gtaattcgca cgaaatttgt ttgtcggacg tagttcggat aaggcgttca
    31381 cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa acgttaattc
    31441 acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta aagcgaaaac
    31501 cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc
    31561 aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca acaagttcaa
    31621 ctacttcatc atgagcaaac tggctgaagc gggtatcccg actcaaatgg agcgtctgct
    31681 ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt gtgtcgtgcg
    31741 taaccgtgct gctggctctc tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa
    31801 cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga tggtcaacga
    31861 atcttactgc gaaacctttg gctgggtgag caaagagaac ctggcgcgta tgaaagagct
    31921 gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc tgattctggt
    31981 cgacttcaag ctggagtttg gcctgtacaa aggtgaagtg gtattgggcg atgagttctc
    32041 cccggacggc agccgcctgt gggacaaaga aacactggag aaaatggaca aagaccgttt
    32101 ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc gtcgcccgcc gcctgggtgt
    32161 acagctggac tgatttttct ggttgcttgt ttctttagcc ggagggtact ccggctaaat
    32221 gtctttgata cccccgctac aatacttctc attatctccc tttgacagga aagtcatacc
    32281 aggccaatat gtatatacat aatgtatatc ccaaaaaggg ggggctatgg aaactactgt
    32341 atttttaagt aatcgcagtc aggccgtcag attaccgaaa gcggtcgctc tgccagaaga
    32401 cgttaagaaa gtagaaatta ttgcgattgg tcgtacaaga atcatcactc ccgccggtga
    32461 gtcctgggac agttggtttg atggcgaaaa tgtgagtgcg gattttatgg atatccgtga
    32521 tcaacccgca atgcaggaaa gggaatcatt ttgatgctga aattcatgct ggataccaat
    32581 atctgtattt tcactattaa gaataaacca gtacatgtca gagagcggtt taacctgaat
    32641 tctggccgga tgtgtataag ttctgtaaca ttgatggagc ttatctatgg ggcagaaaaa
    32701 agtcagatgc cagaacgcaa tttggcggtt attgaaggtt tcgtatctcg tttggtagtg
    32761 cttgattatg atagtgctgc tgcgacgcat accgggcaaa tcagggccga gcttgcccgg
    32821 cagggaaggc ctgttggacc ttttgaccag atgatagcgg gacatgctcg cagtcgcggt
    32881 ttgattgtgg ttaccaacaa tacccgagag tttgagcgag tggcgggaat cagactagaa
    32941 gactggagct aacattctct tttacttcta acattcgtaa attatataac gtggcatgaa
    33001 gccttaattc ctccgccatt tcatcccgct ttcctgtggt aatcttcctc tgaaccacct
    33061 acgatcattt ctataatgaa tatattgttg aggtatctat gcgttggcaa gggcgacgtg
    33121 aaagtgacaa tgttgaagac aggcgcaaca gctccggtgg tccgtctatg ggcggtcctg
    33181 gttttcgcct gccaagcggc aaaggcgggc tgattttact gttagtcgtg ctggttgcag
    33241 gctactatgg tgttgattta accgggttaa tgactggaca gccggtttcc caacaacaat
    33301 caacgcggtc cattagccct aatgaagacg aagctgcaaa attcacctcg gtgattctgg
    33361 caaccacgga agacacctgg ggacaacagt tcgagaagat gggtaagacc tatcagcaac
    33421 cgaagctggt catgtaccgt ggaatgacgc gcaccggctg cggggctggt cagtccatta
    33481 tggggccgtt ctattgcccg gcggatggca cagtttatat cgatctctcc ttctatgatg
    33541 acatgaaaga caaacttggc gcggatggcg attttgccca ggggtacgtt atcgcccatg
    33601 aagtcggtca tcatgtgcag aaactgttag gcatcgagcc gaaagttcgt caactgcaac
    33661 aaaacgcgac gcaggcggaa gtgaaccgct tatctgtgcg tatggaactc caggccgact
    33721 gttttgccgg tgtctggggg catagtatgc agcagcaagg cgttctggaa actggcgatc
    33781 tggaagaggc gctgaatgcg gcgcaggcca tcggcgatga ccgtttacaa cagcaaagtc
    33841 aggggcgagt agtaccagac agtttcacac atggcacttc tcagcaacgc tacagctggt
    33901 ttaaacgtgg tttcgacagc ggcgatccgg cacaatgcaa tacttttggt aaaagcattt
    33961 aatttccggg gcagggatgg ctgaactgac tgcgctttac acattaacag cgcaaatgaa
    34021 acgtgaaggg atccgccgct tgctggtgtt gagcggggaa gagggttggt gttttgatca
    34081 tgcgcttaag ttgcgtgatg ccttacctgg cgactggctg tggatttcgc cgcagccaga
    34141 tgctgaaaac cactgttcac cctcggcgct acaaacttta cttgggcgcg agttccggca
    34201 tgcggtattc gatgcccgcc acggctttga tgccgctgcc tttgcagcac ttagcggaac
    34261 gttgaaagcg ggaagctggc tggttttgtt actccctgta tgggaagagt gggaaaacca
    34321 acctgatgcc gactcgttgc gctggagtga ttgccctgac cctattgcga cgccgcattt
    34381 tgtccagcat ctcaaacgcg tacttacggc ggataacgac gctatcctct ggcggcaaaa
    34441 ccagccgttc tcgttggcgc attttactcc ccgtactgac tggcaccccg cgaccggcgc
    34501 accacaacca gaacaacagc aactcttaca gcagctactg accatgccgc cgggcgtggc
    34561 agcggtaacg gctgcgcgtg ggcgcggtaa atcggcgttg gcagggcaac tcatttctcg
    34621 tattgcgggc agtgcgattg tcaccgcgcc cgcaaaagcg gcaacggatg tactggcaca
    34681 atttgcgggc gagaagtttc gctttattgc gccggatgcc ttgttagcca gcgatgagca
    34741 agccgactgg ctggtggtcg acgaagccgc agccatacct gcgccgttgt tgcatcaact
    34801 ggtatcgcgt tttcctcgaa cgttgttaac cactacggtg cagggctacg aaggcaccgg
    34861 acgtggtttt ttgctgaaat tttgcgctcg ctttccgcat ttacaccgtt ttgaactgca
    34921 acaaccgatc cgctgggcac agggatgccc gcttgaaaaa atggttagtg aggcactggt
    34981 ttttgacgat gaaaacttca cccatacacc acaaggcaat atcgtcattt ccgcatttga
    35041 acaaacgtta tggcgaagcg agccagaaac gccgttaaag gtttatcagt tattgtctgg
    35101 tgcgcactac cggacctcgc cgctggattt acgccggatg atggatgcac cagggcaaca
    35161 ttttttacag gcggctggcg aaaacgagat tgccggggcg ttgtggctgg tggatgaggg
    35221 cggattatct caagaactca gtcaggcggt atgggcaggt tttcgtcgcc cgcggggtaa
    35281 tctggtggcc cagtcgctgg cggcgcacgg cagcaatcca ctggcggcga cattgcgcgg
    35341 aaggcgggtt agccggatag cagttcatcc ggctcgtcag cgggaaggca cagggcggca
    35401 acttattgct ggagctttgc aatatacgca tgacctcgac tatctttcgg tgagttttgg
    35461 ttacaccggg gagttatggc gtttctggca acgctgcggt tttgtgctgg tgcggatggg
    35521 taatcatcgg gaagccagca gcggttgcta tacggcgatg gcgctgttac cgatgagtga
    35581 tgcgggtaaa cagctggctg aacgtgagca ttaccgttta cgtcgcgatg cgcaagctct
    35641 cgcgcagtgg aatggcgaaa cgcttcctgt tgatccacta aacgatgcca tcctttctga
    35701 cgacgactgg cttgaactgg ccggttttgc tttcgctcat cgtccgctat taacatcgtt
    35761 aggttgctta ttgcgtctgc tacaaaccag tgaactggca ttaccggcgc tgcgtgggcg
    35821 tttacagaaa aacgccagtg acgcgcagtt atgtaccaca cttaaacttt caggccgcaa
    35881 gatgttacag gttcgccagc gggaagaggc cgcgcaggcg ctgttcgcac ttaatgatgt
    35941 tcgcactgag cgtctgcgcg atcgcataac gcaatggcaa ttttttcact gactccttca
    36001 attttctccc atggtcattg tgagtcagcg gcgtaaaatt aatgacatca gttaaggagt
    36061 tcgccatgaa acatgaccat tttgttgttc aaagcccgga taaacctgcg caacagttgt
    36121 tactgctttt ccacggtgtc ggggataacc cggtggcaat gggggaaata ggcagttggt
    36181 ttgcaccgtt gttcccggat gcgctggtgg tgagtgtcgg tggcgcggag ccgagcggta
    36241 atccggcggg gcgtcagtgg ttttcggtgc agggtattac ggaagataat cgccaggcgc
    36301 gtgtggatgc catcatgccg acgtttattg agacggtgcg ctactggcag aaacagagcg
    36361 gagtaggggc taacgccacg gcgttaatcg gtttctctca aggcgcgatt atggcgctgg
    36421 agagcattaa agccgaaccg ggccttgctt cacgcgtcat tgcttttaac ggacgctatg
    36481 cgagcctgcc ggaaactgcg tctacagcca cgacaattca cctgattcat ggcggtgaag
    36541 atccggttat cgatctggcg catgcggttg cggcgcagga agcgttaatc agtgccggtg
    36601 gtgacgtgac gctggatatc gtggaggatt taggccatgc gatcgacaat cgcagtatgc
    36661 aatttgccct ggatcacttg cgttatacca ttccgaaaca ttatttcgac gaagcgctaa
    36721 gtggcggtaa gcctggcgat gatgatgtga ttgaaatgat gtaaaagaca gattacctgg
    36781 cctgataaga cgcgtgagcg tcgcatcagg catcgttcac cattgccgga tgcggcgtta
    36841 acgcctaatc cggcctacgg ttgagcactc agcttacttc ttcggttgat cctttttcgg
    36901 ccagtcgtcg tcatcgtccc acttatcgtt gaaatcacga tgcgggggaa gctccggctt
    36961 gttggcgaga aatttcttat ggtcgacgcg cttgagatct ttaatcacgt tcagcaggac
    37021 gcctaccaaa aagacaatca ccagaatcca ccaatattta gccagccagt ccatgcttat
    37081 ttcctcttgc agaaccactc atcaggcgac gagctgttcc atgatacgtt gatacatacg
    37141 ggcaagtagc tgcaggtcgg cagcgttcac acattcatta attttatgaa tagtggcatt
    37201 gaccggcccg agttccacca cctgcgcccc catgcgagca ataaagcgcc cgtcggacgt
    37261 tccgcctgtg gtcagtagct gcggtttaat ttcattatag tgctcaaccg cgttaacgac
    37321 cgcatccacc agtttaccgc gcgcggtcaa aaatggctgc ccggaaagcc accagtccac
    37381 cgtataacgc agttgatgtt tttcaagcag ggcaagcacc tgtgctttga tcatctcatc
    37441 agtcagttcg gtgctgaagc ggaagttaaa ctgcacaaac agttcacccg gaataacatt
    37501 gttactgccc gttcccgcct gaatattagc aatctgcata ctggtcgccg ggaaaaattc
    37561 attgccctga tcccactcaa tagccactaa ttcattaagg aaaggtgctg cgcgatgtac
    37621 tggattgtca gccagatgcg ggtaggccac atgcccctga acgccatgaa tggtaaggtt
    37681 gcaggttaac gatccgcgac gaccattttt caccacatca ccaaccactt cgatactcga
    37741 cggttcgccg accaggcagt aatcgagacg ctcattacgt gccatcaacg cttcgacgac
    37801 ttttaccgta ccgttgtggg cactggcttc ttcatcagag gtgatcagaa atgccagtcg
    37861 ccccgtatgg ttgggatgtt gtgcgacaaa acgttctgcc gctaccacca tcgccgccag
    37921 cgagcctttc atatctgccg caccgcgtcc gaataacatg ccatcacgaa tggtgggttc
    37981 aaacggcgga ttgatccaac gatcggcgtc gccaggcggc accacgtcgg tatgcccggc
    38041 aaaggccaac gtttcacctt gcccacgcca tgcccaaaaa ttctgcgtat cggcaaaatc
    38101 catgcgttca acggtaaaac cgatcgcctg caaacgttca atcaacaaag cctggcatcc
    38161 tgcgtcatca ggactcaggg aagggcggcg aataagctgt tgtgtcagct caataaccgg
    38221 gcacgacata gactacacct catggaaaaa ttgctgataa ctggaatcac tgaaacccag
    38281 cagcataggc ttaccaggcg cgcagagcaa tggacgtttg ataattgcag gcatttcagt
    38341 cattaatgcc gccgcagagg ccgcatcggt gattttattg cgggtggttt cgtccagttt
    38401 acgccaggtt gtaccacggg tgttcagtaa cgcttcccag cctaattcgt tgataaaacc
    38461 gttcaataat tcgctgtcca gcccatcgac gcggtaatca tgaaaacgat agtcgatgtt
    38521 attggcttcc agccaacggc gagccttttt aatggtgtca caatttttga tgccgtaaag
    38581 tgtaaccata atgaatcctt tttcgatcta tgagaataaa tactcattta agggtgaaaa
    38641 cattttgcct gcaa
//
LOCUS       DAMZII010000032        38371 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZII010000032 DAMZII010000000
VERSION     DAMZII010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.032"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            276..1844
                     /gene="cydA"
                     /locus_tag="POA77_001600"
     CDS             276..1844
                     /gene="cydA"
                     /locus_tag="POA77_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI6365058.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1860..2999
                     /gene="cydB"
                     /locus_tag="POA77_001601"
     CDS             1860..2999
                     /gene="cydB"
                     /locus_tag="POA77_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6365059.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3014..3127
                     /gene="cydX"
                     /locus_tag="POA77_001602"
     CDS             3014..3127
                     /gene="cydX"
                     /locus_tag="POA77_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDI6365060.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3127..3420
                     /gene="ybgE"
                     /locus_tag="POA77_001603"
     CDS             3127..3420
                     /gene="ybgE"
                     /locus_tag="POA77_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDI6365061.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3570..3974
                     /gene="ybgC"
                     /locus_tag="POA77_001604"
     CDS             3570..3974
                     /gene="ybgC"
                     /locus_tag="POA77_001604"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI6365062.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3971..4663
                     /gene="tolQ"
                     /locus_tag="POA77_001605"
     CDS             3971..4663
                     /gene="tolQ"
                     /locus_tag="POA77_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDI6365063.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4667..5095
                     /gene="tolR"
                     /locus_tag="POA77_001606"
     CDS             4667..5095
                     /gene="tolR"
                     /locus_tag="POA77_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDI6365064.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5160..6392
                     /gene="tolA"
                     /locus_tag="POA77_001607"
     CDS             5160..6392
                     /gene="tolA"
                     /locus_tag="POA77_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDI6365065.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6525..7817
                     /gene="tolB"
                     /locus_tag="POA77_001608"
     CDS             6525..7817
                     /gene="tolB"
                     /locus_tag="POA77_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDI6365066.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7852..8373
                     /gene="pal"
                     /locus_tag="POA77_001609"
     CDS             7852..8373
                     /gene="pal"
                     /locus_tag="POA77_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDI6365067.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8383..9174
                     /gene="cpoB"
                     /locus_tag="POA77_001610"
     CDS             8383..9174
                     /gene="cpoB"
                     /locus_tag="POA77_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDI6365068.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9339..9414
                     /locus_tag="POA77_001611"
     tRNA            9339..9414
                     /locus_tag="POA77_001611"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9372..9374,aa:Lys,seq:ttt)
     gene            9550..9625
                     /locus_tag="POA77_001612"
     tRNA            9550..9625
                     /locus_tag="POA77_001612"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9583..9585,aa:Val,seq:tac)
     gene            9628..9703
                     /locus_tag="POA77_001613"
     tRNA            9628..9703
                     /locus_tag="POA77_001613"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9661..9663,aa:Lys,seq:ttt)
     gene            9853..9928
                     /locus_tag="POA77_001614"
     tRNA            9853..9928
                     /locus_tag="POA77_001614"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9886..9888,aa:Val,seq:tac)
     gene            9932..10007
                     /locus_tag="POA77_001615"
     tRNA            9932..10007
                     /locus_tag="POA77_001615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9965..9967,aa:Lys,seq:ttt)
     gene            10154..10229
                     /locus_tag="POA77_001616"
     tRNA            10154..10229
                     /locus_tag="POA77_001616"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10187..10189,aa:Lys,seq:ttt)
     gene            10263..10338
                     /locus_tag="POA77_001617"
     tRNA            10263..10338
                     /locus_tag="POA77_001617"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10296..10298,aa:Lys,seq:ttt)
     gene            10369..10494
                     /locus_tag="POA77_001618"
     ncRNA           10369..10494
                     /ncRNA_class="other"
                     /locus_tag="POA77_001618"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10743..11786
                     /gene="nadA"
                     /locus_tag="POA77_001619"
     CDS             10743..11786
                     /gene="nadA"
                     /locus_tag="POA77_001619"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDI6365069.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11824..12543
                     /gene="pnuC"
                     /locus_tag="POA77_001620"
     CDS             11824..12543
                     /gene="pnuC"
                     /locus_tag="POA77_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDI6365070.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12540..13481)
                     /gene="zitB"
                     /locus_tag="POA77_001621"
     CDS             complement(12540..13481)
                     /gene="zitB"
                     /locus_tag="POA77_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDI6365071.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13595..13975)
                     /locus_tag="POA77_001622"
     CDS             complement(13595..13975)
                     /locus_tag="POA77_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDI6365072.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14291..15343
                     /gene="aroG"
                     /locus_tag="POA77_001623"
     CDS             14291..15343
                     /gene="aroG"
                     /locus_tag="POA77_001623"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDI6365073.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15501..16253)
                     /gene="gpmA"
                     /locus_tag="POA77_001624"
     CDS             complement(15501..16253)
                     /gene="gpmA"
                     /locus_tag="POA77_001624"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI6365074.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16458..17498)
                     /gene="galM"
                     /locus_tag="POA77_001625"
     CDS             complement(16458..17498)
                     /gene="galM"
                     /locus_tag="POA77_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDI6365075.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17492..18640)
                     /gene="galK"
                     /locus_tag="POA77_001626"
     CDS             complement(17492..18640)
                     /gene="galK"
                     /locus_tag="POA77_001626"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDI6365076.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18644..19690)
                     /gene="galT"
                     /locus_tag="POA77_001627"
     CDS             complement(18644..19690)
                     /gene="galT"
                     /locus_tag="POA77_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDI6365077.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19700..20716)
                     /gene="galE"
                     /locus_tag="POA77_001628"
     CDS             complement(19700..20716)
                     /gene="galE"
                     /locus_tag="POA77_001628"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDI6365078.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20977..22449)
                     /gene="modF"
                     /locus_tag="POA77_001629"
     CDS             complement(20977..22449)
                     /gene="modF"
                     /locus_tag="POA77_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDI6365079.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPEGDLYRYALTKIN"
     gene            complement(22517..23305)
                     /gene="modE"
                     /locus_tag="POA77_001630"
     CDS             complement(22517..23305)
                     /gene="modE"
                     /locus_tag="POA77_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDI6365080.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23434..23583
                     /gene="acrZ"
                     /locus_tag="POA77_001631"
     CDS             23434..23583
                     /gene="acrZ"
                     /locus_tag="POA77_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDI6365081.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23749..24522
                     /gene="modA"
                     /locus_tag="POA77_001632"
     CDS             23749..24522
                     /gene="modA"
                     /locus_tag="POA77_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6365082.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24522..25211
                     /gene="modB"
                     /locus_tag="POA77_001633"
     CDS             24522..25211
                     /gene="modB"
                     /locus_tag="POA77_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI6365083.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25214..26272
                     /gene="modC"
                     /locus_tag="POA77_001634"
     CDS             25214..26272
                     /gene="modC"
                     /locus_tag="POA77_001634"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDI6365084.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26273..27091)
                     /gene="ybhA"
                     /locus_tag="POA77_001635"
     CDS             complement(26273..27091)
                     /gene="ybhA"
                     /locus_tag="POA77_001635"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDI6365085.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27246..28241
                     /gene="pgl"
                     /locus_tag="POA77_001636"
     CDS             27246..28241
                     /gene="pgl"
                     /locus_tag="POA77_001636"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDI6365086.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28282..29235)
                     /locus_tag="POA77_001637"
     CDS             complement(28282..29235)
                     /locus_tag="POA77_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6365087.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29419..30471
                     /locus_tag="POA77_001638"
     CDS             29419..30471
                     /locus_tag="POA77_001638"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDI6365088.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30547..31980
                     /locus_tag="POA77_001639"
     CDS             30547..31980
                     /locus_tag="POA77_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6365089.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32163..34424
                     /locus_tag="POA77_001640"
     CDS             32163..34424
                     /locus_tag="POA77_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDI6365090.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34658..35941)
                     /locus_tag="POA77_001641"
     CDS             complement(34658..35941)
                     /locus_tag="POA77_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDI6365091.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36076..37146)
                     /locus_tag="POA77_001642"
     CDS             complement(36076..37146)
                     /locus_tag="POA77_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6365092.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(37124..37342)
                     /locus_tag="POA77_001643"
     CDS             complement(37124..37342)
                     /locus_tag="POA77_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI6365093.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(37382..37549)
                     /locus_tag="POA77_001644"
     CDS             complement(37382..37549)
                     /locus_tag="POA77_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365094.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENISDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            37792..>38371
                     /locus_tag="POA77_001645"
     CDS             37792..>38371
                     /locus_tag="POA77_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365095.1"
                     /translation="MSYFLRKKWMVNLSGSGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQRGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPELHEN
                     IRICYPYGNYEYDNIIYVEKKINILGAVVTYAQ"
BASE COUNT         9217 a         9339 c        10034 g         9781 t
ORIGIN      
        1 gaaaggcggt aatttagcta taaattgatc accgtcgaaa aatgcaaatt tgcttcaaca
       61 aaaacctgtt tattgtaagg attttgcggc gtaatatata cgtgggatca atttgagttt
      121 ttattaacat gtttgcaacc tttctttacg ccgtttttgt gtgcattcac atggtatgat
      181 gaaagtgttc aaacaaattt ctattggggc atgcgtgtga ccctttctaa cggggttcac
      241 tctcggagtc ttcatgcgat gagcaaggag tcatgatgtt agatatagtc gaactgtcgc
      301 gcttacagtt tgccttgacc gcgatgtacc acttcctttt tgtgccactg acgctcggta
      361 tggcgttcct gctggccatt atggaaacgg tctacgtcct ctccggcaaa cagatttata
      421 aagatatgac caagttctgg ggcaagttgt ttggtatcaa cttcgctctg ggtgtggcta
      481 ccggtctgac catggagttc cagttcggga ctaactggtc ttactattcc cactatgtag
      541 gggatatctt cggtgcgccg ctggcaatcg aaggtctgat ggccttcttc ctcgaatcca
      601 cctttgtagg tctgttcttc ttcggttggg atcgtctggg taaagttcag catatgtgtg
      661 tcacctggct ggtggcgctc ggttcaaacc tgtccgcact gtggattctg gttgcgaacg
      721 gctggatgca aaacccaatc gcgtccgatt tcaactttga aactatgcgt atggagatgg
      781 tgagcttctc cgagctggtg cttaacccgg ttgctcaggt gaaattcgtt cacactgtag
      841 cgtctggtta tgtgactggc gcgatgttca tcctcggtat cagcgcatgg tatatgctga
      901 aaggtcgtga cttcgccttc gctaaacgct cctttgctat cgctgccagc ttcggtatgg
      961 ctgctgttct gtctgttatt gttctgggtg atgaatccgg ctacgaaatg ggcgacgtgc
     1021 agaaaaccaa actggctgct attgaagccg agtgggaaac gcaacctgcg cctgctgcct
     1081 ttactctgtt cggcattcct gatcaggaag aggagacgaa caaatttgcg attcagatcc
     1141 cttacgcact gggcatcatt gcaacgcgtt ccgtggatac cccggttatc ggcctgaaag
     1201 agctgatggt gcagcatgaa gaacgcattc gtaacgggat gaaggcgtac tctctgctcg
     1261 aacaactgcg ttctggttct accgaccagg cggttcgtga ccagttcaat agcatgaaga
     1321 aagacctcgg ttacggtctg ctgctgaaac gctatacgcc aaacgtggct gatgcgactg
     1381 aagcgcagat tcaacaggca accaaagact ccatcccgcg tgtagcgccg ctgtactttg
     1441 cgttccgtat catggtggcg tgtggcttcc tgctgctggc aatcatcgcg ctctctttct
     1501 ggagtgtcat ccgcaaccgc attggcgaga aaaaatggct tctgcgcgcc gcgctgtacg
     1561 gtattccgct gccgtggatt gctgtagaag cgggctggtt cgtggctgaa tatggccgcc
     1621 aaccgtgggc tatcggtgaa gtgctgccga cagctgtggc gaactcgtca ctgaccgcag
     1681 gcgatctcat cttctcaatg gtgctgattt gcggcctgta taccctgttc ctggtggcag
     1741 aattgttctt aatgttcaaa tttgcacgcc tcggcccaag cagcctgaaa accggtcgct
     1801 atcactttga gcagtcttcc acgactactc agccggcacg ctaagacagg agtcgtcaaa
     1861 tgatcgatta tgaagtattg cgttttatct ggtggctgct ggttggcgtt ctgctgattg
     1921 gttttgcagt cactgacggt ttcgacatgg gggtgggcat gctcacccgt ttcctcggtc
     1981 gtaacgacac cgagcgtcga attatgatta actccattgc accacactgg gacggtaacc
     2041 aggtttggct gatcaccgcg ggcggcgcac tctttgctgc ctggccgatg gtctatgccg
     2101 ctgcgttctc cggcttctat gtggcgatga tcctcgtgct ggcgtctttg ttcttccgtc
     2161 cggtcggttt tgactaccgc tccaagattg aagaaacccg ctggcgtaac atgtgggact
     2221 ggggcatctt cattggtagc ttcgttccgc cgctggtaat tggtgtagcg ttcggtaacc
     2281 tgttgcaggg cgtaccgttc aacgttgatg aatatctgcg tctgtactac accggtaact
     2341 tcttccagtt gcttaacccg ttcggcctgc tggcaggcgt ggtgagcgta gggatgatca
     2401 ttactcaggg cgcaacctat ctgcaaatgc gtaccgtggg cgaactgcac ctgcgtaccc
     2461 gtgcaacggc tcaggtggct gcgctggtga cactggtctg tttcgcactg gctggcgtat
     2521 gggtgatgta cggtatcgat ggttatgtcg tgaaatcgac aatggaccat tacgcagcct
     2581 ctaacccact gaataaagaa gtggttcgtg aagctggcgc atggctggtt aacttcaaca
     2641 acacgccaat tctgtgggct attccggcac tgggtgtggt tctgccgctg ctgaccatcc
     2701 tgactgcacg tatggataaa gccgcgtggg cgtttgtgtt ctcctccctg acgctggcct
     2761 gcatcatcct gacagccggt atcgcaatgt tcccgtttgt gatgccgtcc agcaccatga
     2821 tgaacgcaag tctgacaatg tgggatgcaa cttccagcca gctgacgctt aacgtcatga
     2881 cctgggttgc ggtggttctg gtaccgatca ttctgctcta caccgcctgg tgttactgga
     2941 aaatgttcgg tcgtatcacc aaagaagata ttgaacgtaa cacccactct ctgtactaag
     3001 taaggagcta aaaatgtggt atttcgcatg gattctggga acgcttcttg cctgttcgtt
     3061 tggggtaatc accgcgctgg cgcttgaaca cgtcgaatca ggcaaagccg gtcaagaaga
     3121 catctgatga gtaagattat cgcgactttg tatgcggtaa tggacaagcg ccccctgcgg
     3181 gcgctttcct tcgtgatggc gcttctgtta gcaggatgta tgttttggga cccatctcgt
     3241 tttgccgcga agaccagtga actggaaatc tggcatggtt tattgctgat gtgggccgtc
     3301 tgtgctggtg tgattcacgg cgtgggcttt cgtccgcaga aggttctttg gcaagggatt
     3361 ttttgcccat tgcttgccga tattgttctc attgtcgggc tgattttctt cttcttttaa
     3421 atcagaattc tctttaaaaa attatgggcc gctccaggcc cataaatttt tacgctccct
     3481 taacttgccc tcattcccaa acctcaatcg cgcgcgtata gtagcagcgt ttaaaagctc
     3541 taacttttgt tgcattaccg ggatgtaaag tgaatacaac gctgtttcga tggccggttc
     3601 gcgtctacta tgaagatacc gatgccggtg gtgtggtgta ccacgccagt tacgtcgctt
     3661 tttatgaaag agcacgcaca gagatgctgc gtcatcatca cttcagtcag caggcgctga
     3721 tggctgaacg cgttgccttt gtggtacgta aaatgacggt ggaatattac gcacctgcgc
     3781 ggctcgacga tatgctcgaa atacagactg aaataacatc aatgcgtggc acctctttgg
     3841 ttttcacgca acgtattgtc aacgccgaga atactttgct gaatgaagca gaggttctgg
     3901 ttgtttgcgt tgacccactc aaaatgaagc ctcgtgcgct tcccaagtct attgtcgcgg
     3961 agtttaagca gtgactgaca tgaatatcct tgatttgttc ctgaaggcta gccttctggt
     4021 taaacttatc atgttgattt tgattggttt ttcaatcgca tcttgggcca ttattatcca
     4081 gcggacccgt attcttaacg cagcggcgcg cgaagccgaa gcgtttgaag ataaattctg
     4141 gtctggaatc gaactctctc gcctctatca agagagccag gggaaacggg ataatctgac
     4201 tggttcggaa caaatctttt acagcgggtt caaagagttt gtgcgcctgc atcgtgccaa
     4261 tagccatgcg ccggaagccg tagtggaagg ggcgtcgcgt gctatgcgta tctccatgaa
     4321 ccgtgaactt gaaaatctgg aaacgcacat tccgttcctc ggtacggttg gctccatcag
     4381 cccgtatatt ggtctgtttg gtacggtctg ggggatcatg cacgccttta tcgccctcgg
     4441 ggcggtaaaa caagcaacac tgcaaatggt tgcgcccggt atcgcagaag cgttgattgc
     4501 gactgcaatt ggtctgttcg ccgcaattcc ggcggtaatg gcttataacc gcctcaacca
     4561 gcgcgtaaac aaactggaac tgaattacga caactttatg gaagagttta ccgcgattct
     4621 gcaccgccag gcgtttaccg ttagcgagag caacaagggg taagccatgg ccagagcgcg
     4681 tggacgaggt cgtcgcgatc tcaagtccga aatcaacatt gtaccgttgc tggacgtact
     4741 gctggtgctg ttgctgatct ttatggcgac agcgcccatc atcacccaga gcgtggaggt
     4801 cgatctgcca gacgctactg aatcacaggc ggtgagcagt aacgataatc cgccagtgat
     4861 tgttgaagtg tctggtattg gtcagtacac cgtggtggtt gagaaagatc gcctggagcg
     4921 tttaccacca gagcaggtgg tggcggaagt gtccagccgt ttcaaggcca acccgaaaac
     4981 ggtctttctg atcggtggcg caaaagatgt gccttacgat gaaataatta aagcactgaa
     5041 cttgttacat agtgcgggtg tgaaatcggt tggtttaatg acgcagccta tctaaacatc
     5101 tgcgtttccc ttgcttgaaa gagagcgggt aacaggcgaa cagtttttgg aaaccgagag
     5161 tgtcaaaggc aaccgaacaa aacgacaagc tcaagcgggc gataattatt tcagcagtgc
     5221 tgcatgtcat cttatttgcg gcgctgatct ggagttcgtt cgatgagaat atagaagctt
     5281 cagccggagg cggcggtggt tcgtccatcg acgctgtcat ggttgattca ggtgcggtag
     5341 ttgagcagta caaacgcatg caaagccagg aatcaagcgc gaagcgttct gatgaacagc
     5401 gcaagatgaa ggaacagcag gctgctgaag aactgcgtga gaaacaagcg gctgaacagg
     5461 aacgcctgaa gcaacttgag aaagagcggt tagcggctca ggagcagaaa aagcaggctg
     5521 aagaagccgc aaaacaggcc gagttaaagc agaagcaagc tgaagaggcg gcagcgaaag
     5581 cggcggcaga tgctaaagcg aaggccgaag cagatgctaa agctgcggaa gaagcagcga
     5641 agaaagcggc tgcagacgca aagaaaaaag cagaagcaga agccgccaaa gccgcagccg
     5701 aagcgcagaa aaaagccgag gcagccgctg cggcactgaa gaagaaagcg gaagcggcag
     5761 aagcagctgc agctgaagca agaaagaaag cggcaactga agctgctgaa aaagccaaag
     5821 cagaagctga gaagaaagcg gctgctgaaa aggctgcagc cgacaaaaaa gcagcagaaa
     5881 aagcggctgc tgaaaaggca gcagctgata agaaagcagc ggcagaaaaa gccgccgcag
     5941 acaaaaaagc ggcagcggca aaagctgcag ctgaaaaagc cgctgcagca aaagcggccg
     6001 cagaggcaga tgatattttc ggtgagctaa gctctggtaa gaatgcaccg aaaacggggg
     6061 gaggggcgaa agggaacaat gcttcgcctg ccgggagtgg taatactaaa aacaatggcg
     6121 catcaggggc cgatatcaat aactatgccg ggcagattaa atctgctatc gaaagtaagt
     6181 tctatgacgc atcgtcctat gcaggcaaaa cctgtacgct gcgcataaaa ctggcacccg
     6241 atggtatgtt actggatatc aaacctgaag gtggcgatcc cgcactttgt caggctgcgt
     6301 tggcagcagc taaacttgcg aagatcccga aaccaccaag ccaggcagta tatgaagtgt
     6361 tcaaaaacgc gccattggac ttcaaaccgt aatcgcgatg ttgactgttc ggacggtcaa
     6421 catcaggcac cggttgccac ggggttctgg tagttttgtg tattttagtt tgttaacatt
     6481 ctgctaaatt atcgtgggcc atcggtccag ataagggaga tatgatgaag caggcattac
     6541 gagtagcatt tggttttctc atactgtggg catcagttct gcatgctgaa gtccgcattg
     6601 tgatcgacag cggtgtagat tccggtcgtc ctattggtgt tgttcctttc cagtgggcgg
     6661 ggcctggtgc ggcacctgaa gatattggcg gcatcgttgc tgctgacttg cgtaacagcg
     6721 gtaaatttaa tccgttagat cgcgctcgtc tgccacagca gccgggtagt gcgcaggaag
     6781 tacaaccagc tgcatggtcc gcactgggca ttgacgctgt agttgtcggt caggtcactc
     6841 cgaatccgga tggttcttac aatgttgctt atcaacttgt tgacactggc ggcgcaccgg
     6901 gtactgtact tgctcagaac tcgtacaaag tgaacaagca gtggctgcgt tatgctggtc
     6961 ataccgccag tgatgaagtg tttgaaaaac tgaccggcat taaaggtgcg ttccgtactc
     7021 gtattgccta cgttgttcag accaacggcg gtcagttccc gtatgaactg cgcgtatctg
     7081 actatgacgg ttacaaccag tttgtcgttc accgttcacc gcagccgctg atgtctccgg
     7141 cgtggtcacc agacggttct aaactggctt atgtgacctt cgaaagcggt cgttccgcgc
     7201 tggttattca gacgctggca aatggcgctg tacgtcaggt ggcttcattc ccgcgtcaca
     7261 acggtgcacc tgcattctcg ccagacggca gcaaactggc attcgccttg tcgaaaaccg
     7321 gtagtctgaa cctgtacgta atggatttgg cttctggtca gatccgccag gtgactgatg
     7381 gtcgcagtaa caataccgaa ccgacctggt tcccggatag ccagaacctg gcatttactt
     7441 ctgaccaggc cggtcgtccg caggtttata aagtgaatat caacggcggt gcgccacaac
     7501 gtattacctg ggaaggttcg cagaaccagg atgcggatgt cagcagcgac ggtaaattta
     7561 tggtaatggt cagctccaat ggtgggcagc agcacattgc caaacaagat ctggcaacgg
     7621 gaggcgtaca agttctgtcg tccacgttcc tggatgaaac gccaagtctg gcacctaacg
     7681 gcactatggt aatctacagc tcttctcagg ggatgggatc cgtgctgaat ttggtttcta
     7741 cagatgggcg tttcaaagcg cgtcttccgg caactgatgg acaggtcaaa ttccctgcct
     7801 ggtcgccgta tctgtgataa taattaattg aatagtaaag gaatcattga aatgcaactg
     7861 aacaaagtgc tgaaagggct gatgattgct ctgcctgtta tggcaattgc ggcatgttct
     7921 tccaacaaga acgccagcaa tgacggcagc gaaggcatgc tgggtgccgg cactggtatg
     7981 gatgcgaacg gcggcaacgg caacatgtct tccgaagagc aggctcgtct gcaaatgcaa
     8041 cagctgcagc agaacaacat cgtttacttc gatctggaca agtacgatat ccgttctgac
     8101 ttcgctcaaa tgctggatgc acatgcaaac ttcctgcgta gcaacccgtc ttacaaagtc
     8161 accgtagaag gtcacgcgga cgaacgtggt actccggaat acaacatctc cctgggtgaa
     8221 cgtcgtgcga acgccgttaa gatgtacctg cagggtaaag gcgtttctgc agaccagatc
     8281 tccatcgttt cttacggtaa agaaaaacct gcagtactgg gtcatgacga agcggcatac
     8341 tccaaaaacc gtcgtgcggt actggtttac taagagaatt gcatgagcag taacttcaga
     8401 catcaactat tgagtctgtc gttactggtt ggtatagcgg ccccctgggc cgcttttgct
     8461 caggcaccaa tcagtagtgt cggctcaggc tcggtcgaag accgcgtcac tcaacttgag
     8521 cgtatttcta atgctcacag ccagctttta acccaactcc agcaacaact ttctgataat
     8581 caatccgata ttgattccct gcgtggtcag attcaggaaa atcagtatca actgaatcag
     8641 gtcgtggagc ggcaaaagca gatcctgttg cagatcgaca gcctcagcag cggtggtgca
     8701 gcggcgcaat caaccagcag cgatcaaagc ggtgcggcgg catcaacgac gccgacagct
     8761 gatgctggta ctgcgaatgc tggcgcgccg gtgaaaagcg gcgatgcaaa cacggattac
     8821 aatgcagcta ttgcgctggt gcaggataaa tcccgccagg atgacgcaat ggtggcattt
     8881 cagaatttca tcaaaaatta ccctgattca acttacctgc caaacgccaa ttattggctg
     8941 ggtcagttaa actacaacaa gggtaaaaaa gatgatgcgg cgtactattt tgcttcggta
     9001 gtgaaaaact atccgaagtc accaaaggct gctgatgcga tgtttaaagt cggcgtcatc
     9061 atgcaggaca aaggtgacac cgcaaaagcg aaagccgtat accagcaggt tatcagtaaa
     9121 taccctggta ctgatggcgc taaacaggca caaaaacgtc tgaacgcgat gtaatgcata
     9181 acacacgacc agaagtcgca ttatttctgg tcgtgtcgtg cgaatcataa gcagttgagt
     9241 gatctgcatc gaaatttttg ttgcgctcaa gtctgaaatc agtaatatat gccgccgttg
     9301 ccacgggata tcaaacaaac cgaaagcaac gaaaaagtgg gtcgttagct cagttggtag
     9361 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatcgct
     9421 aaggtggaag cggtagtaaa acgtgaagga taacgttgca tcagcaacgg cccgaagggc
     9481 gagacgaagt cgagtcatcc tgcacgaccc accactaaca tagttagttg tagtatccag
     9541 cgtagtattg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
     9601 ggttcgatcc cgtcatcacc caccaccggg tcgttagctc agttggtaga gcagttgact
     9661 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccagttttag catcgaagac
     9721 agatgttaag cgtgtaggat aacgttgcgt cagcaacggc ccgtagggcg agcgaagcga
     9781 gtcatcctgc acgacccacc actaattacg gtgggttcgg tggaagtagt ttgtagtatc
     9841 cagcgcagta tcgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
     9901 ggcggttcga tcccgtcatc acccaccact cgggtcgtta gctcagttgg tagagcagtt
     9961 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatt ttaacatcaa
    10021 actcagatgt taagcgtgaa ggataacgtt gcgccagcaa cggcccgtag ggcaagcgaa
    10081 gcgagtcatc ctgcacgaca caccaatctt aaagattggc cccgagtaga aatctttcag
    10141 gtaacacccg tatgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
    10201 cgcaggttcg aatcctgcac gacccaccat cctgaatgat taaagcagca taatcccgca
    10261 aggggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga
    10321 atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga
    10381 tactcaaaga ttcgaacctg cagcaggttt gagttgagcg cagcgaaaca acggagccgc
    10441 tcgcggcgac ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc accaatgtaa
    10501 aaaagcgccc taaaggcgct tttttgctat tcaggcatcc tcaatttcac tttgtaaacc
    10561 tgatgacatc gtcagagctt actgtgcaag caactctacg tcggtggaat taggcgtaat
    10621 tcgagtgact tttccccacc attcgactat cttgtttagc atataaaaca aattacaccg
    10681 ataacagcga atattacgct aatgtcggtt ttaacgttaa gcctgtaaaa cgagatggta
    10741 agatgagcgt aatgtttgat ccagacacgg cgatttatcc tttccccccg aagccgacgc
    10801 cgttaagcat tgatgaaaaa gcgtattacc gcgagaagat aaaacgtctg ctaaaagaac
    10861 gtaatgcggt gatggttgcc cactactata ccgatcccga aattcaacaa ctggcagaag
    10921 aaaccggtgg ctgtatttct gattctctgg aaatggcgcg cttcggtgca aagcatcccg
    10981 cttctacttt gttagtcgct ggggtgagat ttatgggaga aaccgccaaa attctcagtc
    11041 cggaaaaaac aattctgatg ccgacacttc aggctgaatg ttcactggat ctcggctgcc
    11101 ctgttgaaga atttaacgca ttttgcgatg cccatcccga tcgtactgtc gtcgtctacg
    11161 ccaacacttc tgctgcggta aaagcgcgcg cagattgggt ggtaacttca agcattgccg
    11221 tcgaacttat tgatcatctt gatagtttgg gtgaaaaaat catctgggca cccgacaaac
    11281 atctggggcg ttacgtgcaa aaacagacgg gtggagacat tctatgctgg cagggtgcct
    11341 gtattgtgca tgatgaattt aagactcagg cgttaacccg cttgcaagaa gaatacccgg
    11401 atgctgccat actggtgcat ccagaatcac cacaagctat tgtcgatatg gcggatgcgg
    11461 tcggttccac cagtcaactg atcgctgctg cgaaaacatt gccacatcag cggcttatcg
    11521 tggcaaccga tcggggcatt ttctacaaaa tgcagcaggc agtgccagat aaagagttac
    11581 tggaagcacc aaccgcaggt gagggtgcaa cctgccgcag ctgcgcgcat tgtccgtgga
    11641 tggccatgaa tggccttcag tccatcgcag aggcattaga acaggaagga agcaatcacg
    11701 aggttcatgt tgatgaaagg ctgcgagaga gggcgctggt gccgctcaat cgtatgctgg
    11761 attttgcggc tacactacgt ggataacgaa taataaggcg taacgttacg ctttggggga
    11821 aagatggatt tttttagtgt gcagaatatc ctggtacata taccaatagg ggcaggcggt
    11881 tatgatctct catggatcga agcggtaggc acgatcgccg ggttgctgtg tattggcctt
    11941 gccagtctgg agaagatcag caactacttc tttggcctga tcaacgtcac cttgtttggc
    12001 attattttct ttcagattca gctgtatgcc agcctgctat tacaggtgtt tttctttgcc
    12061 gcgaatattt acggttggta tgcgtggtcg cgacaaacca gtcagaacga ggcggagttg
    12121 aaaattcgct ggttgccatt gccgaaggca ctcagctggt tggcggtttg cgttgtttcg
    12181 attggtctga tgacggtatt tatcaatccg gtgtttgcat ttttgacccg cgtggcagtc
    12241 atgatcatgc aagcattagg attacaggtt gtgatgcctg aactgcaacc ggacgctttc
    12301 ccgttctggg attcatgcat gatggtgtta tctatcgtgg caatgattct gatgacgcgt
    12361 aagtatgtgg aaaactggct gttgtgggtg attattaacg tgattagcgt cgttattttt
    12421 gcacttcagg gcgtttacgc catgtctctg gagtacatca tcctgacctt tattgcgctc
    12481 aacggcagcc ggatgtggat caacagcgca cgtgaaagag gctcacgcgc gctgtcccat
    12541 taatggtgat gatgtgaatg gccagacacc ccttcattca ggtggcagtc tggcccatga
    12601 caaggttgat attccatctg aatggtggcg tgctcaatct gatagtgatc catcaggtag
    12661 tgttggatct gatccaacaa ggcatcgtga tcgtgtggcg ggatcacctg cacatgcaac
    12721 gtcatcaccg gcttctcgcc taccatccat acatgtacat ggtgcacatt gcggacttcc
    12781 gggatttccc ggcacatacg acgcttcagt tcagcgatat ccagcgatac cggtgcacct
    12841 tcaagtaatt cattcacgct atctttcaac agtcgccacg cgctacgcag aaccagaagc
    12901 gacaccagta tcgagagaat ggggtcagca ggtgtccagc cagtccagat aataatcaac
    12961 gcggcaataa tagccccaac cgaacccagc aggtccccga gcacatgcag tgccgctgct
    13021 cgtacgttga ggtttttctc ttcgctgccg tgatgaagta accagaaaga aagtatattt
    13081 gccagcaacc cagccacggc aattgccatc atcatgccgc cctcgaccgg acgcggcgtg
    13141 cggaagcgtt ctatcgcctc ccagacaatc aaaatggtaa ttaccacgag ggctatggca
    13201 ttcacaaacg ccgcgagcgt ggtcagtctt agccaaccga aagtgtggcg aatggtggga
    13261 ggacgacgtg aaaattgcac ggcgagtagg gcgaaaagca gggcggcagt atcggtcaac
    13321 atatgacccg catcggccag caatgccaga gaaccagaaa gaaaaccacc aacgacttct
    13381 accagcataa acccggcagt cacgccgaaa gcatacaaca agcgacgagc attattatct
    13441 tctggcaggt gtgaggacgt gtgtgagtgt gagtgcgcca tgacgagtca tcccttaatt
    13501 attctcactt aactttatga catcatactg cttttagaag tgaaaaatta aaagggagag
    13561 actccgctct cccattattg gctattttgc agggttactg cgtggtaccg tcggttttgg
    13621 tatcgacatc attattgatg ccatcaccgg tttgtacctt tttattgata tccggacagc
    13681 gaccatcttt gcacatggtg ttcttgtgct cttcatcttt ggtcattccg tcattgttca
    13741 ttgaagaacc atccgaatgc agcattgtgc caccagaacc ggtatttacc ccgttattgt
    13801 cgacgttatt tggcgcgaca ttttcacggg cgtcaggggc tacctggccc gcatcagctg
    13861 cggcgtttgc ctggccgtta ttagtttgcg ctccgctatc ggcagccagt gcggcaccgc
    13921 tggcaaggct tagagtggca gtcagaaata atgtggccag ttttgtcatt ttcataggat
    13981 gctcctgtta tggtcgttat gtcggataac ctcttccaac agtgcatttg caggtgaata
    14041 taaggcattg gtttaagatt tcagccaggt tatgaaacgc agcagagaat cttgaaataa
    14101 ttaacaaaca aaggagttac agttagaaat tgtaggagag atctcgtttt tcgcgacaat
    14161 ctggcgtttt tcttgctaat tccaggatta atccgttcat agtgtaaaac cccgtttaca
    14221 cattctgacg gaagatatag attggaagta ttgcattcac taagataagt atggcaacac
    14281 tggaacagac atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact
    14341 tcctcctgtc gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggttgc
    14401 ccatgcccga aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc tgttggttgt
    14461 gattggccca tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca ctcgcttgct
    14521 ggcgctgcgt gaagagctga aagatgagct ggaaatcgta atgcgcgtct attttgaaaa
    14581 gccgcgtacc acggtgggct ggaaagggct gattaacgat ccgcatatgg ataatagctt
    14641 ccagatcaac gacggtctgc gtatagcccg taaattgctg cttgatatta acgacagcgg
    14701 tctgccagcg gcaggtgagt ttctcgatat gatcacccca caatatctcg ctgacctgat
    14761 gagctggggc gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg aactggcatc
    14821 agggctttct tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta aagtggctat
    14881 cgatgccatt aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca
    14941 ttcggcgatt gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa
    15001 agagcctaac tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg
    15061 cctgccagca caggtgatga tcgatttcag ccatgctaac tcgtccaaac aattcaaaaa
    15121 gcagatggat gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat
    15181 tggcgtgatg gtggaaagcc atctggtgga aggcaatcag agcctcgaga gcggggagcc
    15241 gctggcctac ggtaagagca tcaccgatgc ctgcatcggc tgggaagata ccgatgctct
    15301 gttacgtcaa ctggcgaatg cagtaaaagc gcgtcgcggg taaggtttaa ttgtcggatg
    15361 cgccgtcaga gtggcgtatc cgatgaatca ccacaggcct gataagtcgc gcagcgtcgc
    15421 atcaggcaat gtgctccatt gttagcaaca aaaatgccga ctcacttgca gtcggctttc
    15481 tcattttaaa cgaatgacgt ttacttcgct ttaccctggt ttgcaaccgc cgctgctttc
    15541 gctgcgatct cgtcagcatt acccagataa tagcgtttca gcggtttgaa attctcgtcg
    15601 aactcataca ccagcggcac gccagtcggg atattaagct caagaatctc ttcttcgctc
    15661 atgttatcaa gatatttcac cagcgcacgt aaagagttac cgtgtgcagc gatgatcacg
    15721 cgctcaccgc tcttcatacg cggcagaata gtttcattcc agtaagggat cacgcggtca
    15781 atggtcagcg ccaggctttc cgtcagcggc agttcttttt cgctcagttt cgcgtaacgc
    15841 ggatcgtgac ccggataacg ctcatcatct ttagtcagtt ccggcggagt cactgcaaaa
    15901 ccacgacgcc actgtttcac ctgctcgtcg ccatactttt cagcagtttc cgctttgttc
    15961 agaccctgca acgcaccgta gtgacgttcg ttcagtttcc aggatttctc aacgggcagc
    16021 catgcctgat ccagttcgtc cagcacattc cacagggtat ggatagcgcg tttcagcaca
    16081 gaggtgtaag caaagtcaaa gctgtaacct tcctctttca gcagcttacc tgctgctttt
    16141 gcttcgctta cgcctttctc agacagatcc acgtcgtacc aaccggtgaa acggttttct
    16201 ttgttccact gactttcgcc atgacgaacc agaaccagct tagttacagc catatactta
    16261 ctcctcaaat cattttttaa tgataataat tctcattata ttgccgtgac gaagcaacag
    16321 caatgcttac gcataaccat agcgaaaata gtgtcgcagt gtaaggttgt tgtgaatatt
    16381 gagttgcaaa tatgtcggtg tttgctggtg atttgagcaa tataaggata aagccctcat
    16441 tacgagggct tcaggtgcta ctcagcaata aactgatatt ccgtcaggct ggaatactct
    16501 tcgccaggac gcaggaagca gtccggttgc ggccattcag ggtggtttgg gctgtccggc
    16561 agaaactcgc tttccagtgc caggccttgc cagtcggcgt aaggttcagt tcctcttgat
    16621 ggcgtgccgc cgaggaagtt gccggagtag aattgcagag ccggagcggt ggtgtagacc
    16681 ttcagctgca atttttcatc tgctgaccag acatgcgccg ccactttctt gccatcgcct
    16741 ttggcctgta acaagaatgc gtgatcgtaa cctttcactt tgcgctgatc gtcgtcggca
    16801 agaaactcac tggcgatgat tttggcgcta cggaaatcaa aagacgttcc ggcgacagat
    16861 ttcaggccgt cgtgcggaat gccgccttca tcaaccggca gatattcgtc cgccagaatc
    16921 tgcaacttgt gattgcgcac gtcagactgc tcgccgtcaa gattgaaata gacgtgatta
    16981 gtcatattca ccgggcaagg tttatcaact gtggcgcgat aagtaataga gatacggtta
    17041 tcgtcggtca gacgatattg caccgtcgcg ccgagattac ccgggaagcc ctgatcgcca
    17101 tcatctgaac tcagggcaaa cagcacctga cgatcgttct ggttcacaat ctgccagcga
    17161 cgtttgtcga acccttccgg gccgccgtgc agctggttaa cgccctgact tggcgaaagg
    17221 gtcacggttt caccgtcaaa ggtataacgg ctattggcga tgcggttggc ataacgacca
    17281 atagaggccc ccagaaacgc ggcctgatcc tgatagcatt ccgggctggc acagccgagc
    17341 agcgcctcgc ggacgctgcc atcggaaagc ggaatacggg ctgaaagtaa agtcgcaccc
    17401 cagtccatca gcgtgactac catccctgcg ttgttacgca aagttaacag tcggtacggc
    17461 tgaccatcgg gtgccagtgc gggagtttcg ttcagcactg tcctgctcct tgtgatggtt
    17521 tacaaacgta aaaagtctct ttaatacctg tttttgcttc atattgttca gcgacagctt
    17581 gctgtacggc aggcaccagc tcttccggga tcagcgcgac gatacagccg ccaaatccgc
    17641 cgccggtcat gcgtacgcca cctttgtcgc caatcacagc tttgacgatt tctaccagag
    17701 tgtcaatttg cggcacggtg atttcgaaat catcgcgcat agaggcatga gattccgcca
    17761 tcaactcgcc catacgtttc aggtcgcctt gctccagcgc gctggcagct tcaacggtgc
    17821 gggcgttttc agtcaggata tgacgcacgc gtttcgccac gattgggtcc agctcatgtg
    17881 caacagcgtt gaactcttca atggtgacat cgcgcagagc tggctgctgg aagaaacgcg
    17941 caccggtttc gcactgttca cgacgggtgt tgtattcgct gccaaccagg gtacgtttga
    18001 agttactgtt gatgatgacg acagccacac ctttcggcat ggaaactgct ttagtcccca
    18061 gtgagcggca gtcaatcagc aaggcatgat ctttcttgcc gagtgcggaa attagctgat
    18121 ccatgatccc gcagttacag ccaacaaact ggttttctgc ttcctgaccg ttaagcgcga
    18181 tttgtgcgcc gtccagcggc agatgataaa gctgctgcaa tacggttccg accgcgactt
    18241 ccagtgaagc ggaagaactt aacccggcac cctgcggcac attgccgctg atcaccatgt
    18301 ccacgccgcc gaagctgttg ttacgcagtt gcagatgttt caccacgcca cgaacgtagt
    18361 tcgcccattg atagttttca tgcgcgacaa tgggcgcatc gagggaaaac tcgtcgagct
    18421 gattttcata atcggctgcc attacgcgaa ctttacggtc atcgcgtggc gcacagctga
    18481 tcacggtttg ataatcaatc gcgcagggca gaacgaaacc gtcgttgtag tcggtgtgtt
    18541 caccaatcaa attcacgcgg ccaggcgcct gaatggtgtg agtggcaggg tagccaaatg
    18601 cgttggcaaa cagagattgt gttttttctt tcagactcat ttcttacact ccggattcgc
    18661 gaaaatggat atcgctgact gcgcgcaaac gctctgctgc ctgttctgcg gtcaggtctc
    18721 gctgggtctc tgccagcatt tcataaccaa ccataaattt acgtacggtg gcggagcgca
    18781 gcagaggcgg ataaaagtgc gcgtgcagct gccagtgttg attctcttcg ccattaaatg
    18841 gcgcgccgtg ccagcccata gagtagggga aggagcactg gaagaggttg tcataacgac
    18901 tggtcagctt tttcaacgcc agtgctaaat cgctgcgctg ggcatcggtc aaatcggtga
    18961 tccgtaaaac gtgggctttg ggcagcagta gcgtttcgaa cggccaggca gcccagtaag
    19021 gcactacggc taaccagtgt tcggtttcga caacggtacg gctaccgtct gccagctcgc
    19081 gctgaacata atccaccagc attggcgatt tctgcccggc aaaatattct ttttgcaggc
    19141 ggtcttcgcg ctcagcttcg ttaggcagga agctatttgc ccaaacctgt ccgtgcggat
    19201 gcgggttaga gcagcccatc gccgcacctt tgttttcaaa gacctgcacc cacgggtacg
    19261 tttttcccag ctctgcggtt tgctcctgcc aggttttgac gatttccgtc aatgccgcaa
    19321 cgctcagttc tggcaacgtt ttactgtgat ccggtgaaaa gcagatcacc cggctggtac
    19381 cgcgcgcgct ctggcaacgc atcaaaggat cgttgctttc tggcgcatct ggcgtgtcag
    19441 acatcaaggc cgcaaaatca ttagtgaaaa cgtaagtccc ggtgtaatcg gggtttttat
    19501 cgcctgtcac ccgcacatta cctgcgcaga ggaagcaatc tggatcgtgc gcaggtaaca
    19561 cctgtttggc tggcgtttcc tgcgccccct gccaggggcg cttagcgcgg tgcggtgaaa
    19621 ccagaatcca ttgcccggtg agcgggttgt agcggcgatg tggatgatca acgggattaa
    19681 attgcgtcat ggtcgttcct taatcgggat atccctgtgg atggcgtgac tgccagtgcc
    19741 aggtgtcctg cgccatttca tcgagtgtgc gcgttacgcg ccagttcagt tcgcgatcgg
    19801 ctttgctggc gtccgcccag taggccggaa ggtcgccctc gcgacgcggt gcaaaatgat
    19861 agttaaccgg tttgccgcag gctttgctga aggcattaac cacgtccagc acgctgctgc
    19921 ctacgccagc gccgaggttg tagatgtgta cgcctggctt gttcgccagt ttttccatcg
    19981 ccacgacgtg accgtccgcc agatccatta cgtggatgta atcgcgtacg ccagtaccat
    20041 cttcggtcgg ataatcgtta ccaaaaatcg ccagcgagtc gcgacggcct acagcaacct
    20101 gggcgatgta tggcatcagg ttattcggaa tgccttgcgg atcttcaccc atatcgcccg
    20161 acggatgcgc gccaaccggg ttgaagtagc gcagcagggc aatgctccag tccggctggg
    20221 ctttttgcag gtcggtgagg atctgttcca ccatcaattt gcttttgcca taagggcttt
    20281 gcggtgtgcc ggtcgggaag ctttcaacgt atggaatttt gggctgatcg ccataaacgg
    20341 tggcggagga gctaaaaata aagtttttga cgttagcggc gcgcatggcg ctaatcaggc
    20401 gcagagtgcc gttgacattg ttgtcgtaat attccagcgg tttttgtacc gattcgccaa
    20461 cggctttcag cccggcgaag tggatcacgg tgtcgatagc gtgatcgtgc aggatctcgg
    20521 tcatcaacgc ttcgttacga atatcgcctt caacaaacgt tggatgtttg ccgcctaaac
    20581 gctcgataac aggcagtacg ctgcgcttac tgttacagag gttatcaaga atgatgacat
    20641 catgaccgtt ttgcagtaat tgcacacagg tatgacttcc aatgtaaccg ctaccaccgg
    20701 taaccagaac tctcataatt cgctccatta ggcttatggt atgaaataac catagcataa
    20761 caaagatgcg aaaagtgtga catggaataa attagtggaa tcgtttacac aagaatttag
    20821 ccgtttttta tgcgcgatta agtgattata aaacagaggg tttatgaatg attgcgcttt
    20881 ttatctgaaa aaagacgcgg tttcatgcct gcatgcgtcg aactgtaggc cggagagggg
    20941 gcttacgcca catccggcac agtcagcatt accaactcag tttatttttg tcagcgcata
    21001 gcgataaagg tcaccttccg gcacgaactc aagacgatgg gtaatacagg caggcgcatc
    21061 ttcagcgtgg tgcgaaacaa acaacaattg cgtttcacct tcgctaatca gcacatcaac
    21121 aaaacggcgg ataagttggc gattgagcgg atcaagcccc tgtagtggtt catcgagaat
    21181 aagcaatgtc ggatgtttca ccagcgcgcg gacgatcagc gccagacgct gctgtcccca
    21241 ggaaagacta tggaacggaa cgtcagccgt gcgtttatca atgccgagaa tatccagcca
    21301 ctgctgcacc agtttttgct ggcgatccga aacggcctga taaatgccaa tcgaatcaaa
    21361 atagccagaa agaatcacat tacgcacggt agtgctgacc cggtaatcca gatgcaaact
    21421 actgctgacg taaccgatat gctttttgat atcccagatg gtttcgccgc tgccgcgacg
    21481 tcggccgaaa agcgtcaaat cgttgctgta accttgcgga tgatcgccag taatcaggct
    21541 taataacgtc gattttcccg caccatttgg cccgacaatt tgccagtgct cgccaggatt
    21601 cacctgccag ctaaggttat taagaatggg gcgatcgtta taagaaacca cgccattatt
    21661 cagcacaatg cgcggttcgt tggtgggtaa ggcgtgacgt gctgaaggtt catccggctc
    21721 cggcagttgc acaccttcaa gctgttcgct atgcgccagt tgcgcgacga gtgcttgctg
    21781 gagcagttcc tctttagcgc ccgtttccgc taacgtacaa tccgccagca cgccagcaaa
    21841 ctggacaaat tccgggatct catcgaagcg attgagcacc agtaccagag taataccgga
    21901 ctgatgtaac gaggcgagtc gctcagccag ctgctgacgt gaggcaacat ccaggccatc
    21961 gaacggctca tcaagaatca acaagtcagg ctccgacatc agcgcctgac acagcagggt
    22021 ttttcgcgtc tcgccagtgg aaaggtattt aaagcgtcgg tcgaggaggg cggtaatacc
    22081 gaactgctgc gccagttgca tgcaacgcgg tgcatccttt acttcatcct gaataatctc
    22141 agccgtagtg cgtccggtgt catcttcgcc agggccgagc atatcggtgt tattccgctg
    22201 ccattcgtcg ctgacgagtt tttgcaattg ctcgaaggag agacgagtga tgtgggaaaa
    22261 ctggctttgc cgttcacctt tcaaaagcgg aagttccccc gccagcgcgc gggccagcgc
    22321 cgatttcccg cttccattcg aaccgacaaa cgcccaacta tcacccgcgt ttaacgttag
    22381 ctgaggcaat tgcagcgttt ttgtgtcgct aagacgaaac gtgccttgca aaatttgcaa
    22441 cgatgacatt ttatatccca ttttgtgcag cgattactga cagggatacg tgtttcataa
    22501 caaattgtca acacgcttag cacagcgtgg cgataatcac gctgtcggca ttaaagtagg
    22561 ccgtgacatt ctgcccttgc tgaagagaag tcgcttcatt taccggcact gtggcgcaca
    22621 gtgtttgccc gtcgggtagc gccattaata cttcgcactg ctctgcgccg cgctcaatat
    22681 gactaataat acccggtaat tggttgtcag cgttttgcgc gaccgcctcg tcctgagtaa
    22741 tacctaccca cggcgctttt agcaatatca acacttcttt gccttcatcc agccccagac
    22801 gcgcgccgct ttgtgcggta attgcgactt tcaggcgtgt ttttccgtca gccagtaaga
    22861 catcaacatg ctgttgaacg tcatcatgat cgcgggcggt gatggtgccg aaccactggt
    22921 tacgggcgct ggtttgcagt gaaaaacgtg agatcgcggc cagcaggctg ttcagcggca
    22981 gggcgtcatc gtcacttaac acatcaaagg ctttttgctg gatttgcgcc agtaagtcat
    23041 agagctgaat cagtcgctga ccatagcggg tcagtactgc gccgccgcca cctttaccgc
    23101 ctgttgcgcg ctcgaccaga atatgctcac ttaactgatt catctcgtta atggcatccc
    23161 aggcgctttt atagctaata ccggcatctt tcgctccctg gctaatggaa ccggaaagcg
    23221 caatgtgttt tagtagcgaa atgcggcgcg ggtcggcgaa taatttttgt tggagcttaa
    23281 gggtgagaag gatttcggcc tgcataacaa tgtcctggca aaagtcttat tgtgacggaa
    23341 aacgaacgcc acgcaaagct gaccgcacaa aaggggagtg cttttctgtg cttagcggtt
    23401 agaatagtct catgactata tctggagttg accatgttag agttattaaa aagtctggta
    23461 ttcgccgtaa tcatggtacc tgtcgtgatg gccatcatcc tgggtctgat ttacggtctt
    23521 ggtgaagtat tcaacatctt ttctggtgtt ggtaaaaaag accagcccgg acaaaatcat
    23581 tgattccctg aatgcccgct tagtcgggca tttctttttc tcaacttcct gcttttcctg
    23641 ccgatatttt ttcttatcta cctcacaaag gttagcaata actgctggga aaattctgag
    23701 ctagccgtta tattgtcgcc tacataacgt tacattaagg ggttaccaat ggctcgtaaa
    23761 tggttgaact tgtttgccgg ggcggcactc tctttcgctg ttgctggcaa tacactggca
    23821 gatgaaggga aaatcacggt gttcgccgcc gcatcactga ctaacgcaat gcaggacatt
    23881 gctacgcagt ataaaaaaga aaaaggcgtg gatgtggttt cttctttcgc ttcgtcatct
    23941 actctggccc gtcagattga agcgggagcg cctgcggatc tgtttatctc tgccgatcag
    24001 aaatggatgg attatgcggt tgataaaaaa gcgatcgata cggcttcgcg ccagacactg
    24061 ctcggcaata gcctggtcgt tgtagcaccg aaagccagcg agcagaaaga tttcatcatc
    24121 gacagcaaaa ccaactggac ttcgctgctg aatggcggtc gcctggcggt tggcgatccg
    24181 gaacatgttc ccgctggcat ttatgcaaaa gaagcactgc aaaaactggg cgcatgggat
    24241 acgctctctc cgaaactggc tccggcggaa gatgttcgtg gcgcgctggc gttggtcgaa
    24301 cgcaatgaag cgccgctggg catagtttac gggtctgacg cagttgccag caaaggggta
    24361 aaagtggtcg ccaccttccc ggaagattcg cataaaaaag tggaataccc ggttgctgtt
    24421 gtggaagggc ataacaatgc gacagtgaaa gcattttatg attatttgaa agggccgcag
    24481 gctgcggaaa tctttaaacg ttacggattt acaaccaagt aatgatactg accgatccag
    24541 aatggcaggc agttttatta agcctgaaag tttcttccct ggctgtgctg tttagcctgc
    24601 cgtttgggat cttttttgcc tggttactgg tgcgttgcac gtttccgggc aaagctctgc
    24661 tcgacagcgt actgcatcta ccgctggtgt taccgcccgt ggtcgtcggt tacttattat
    24721 tagtttcgat gggacggcgc gggtttatcg gtgaacgtct gtatgactgg tttggcatta
    24781 ctttcgcctt tagctggcgc ggcgcggttc tcgctgccgc cgtcatgtcg tttccgctga
    24841 tggtgcgggc aattcgtctg gcgctggaag gggttgatgt caaactggaa caggccgcaa
    24901 gaacactggg ggccgggcgc tggcgcgttt tctttactat cacgataccg ctgaccttac
    24961 cgggaattat tgttggtacg gtactggctt ttgctcgttc tctcggtgag tttggtgcaa
    25021 ccatcacctt tgtgtcgaac attcctggtg aaacgcgaac cattccttct gccatgtata
    25081 ccctgatcca gacccccggc ggggaaagtg gcgcggcgag actgtgcatt atttctattg
    25141 cgctggcgat gatctccctg ttgatttcag aatggctggc cagaatcagc cgtgaacggg
    25201 cggggcgcta atcatgctgg aactgaattt ttcccagacc ttgggcaacc attgcctgac
    25261 cattaatgaa acgctgcccg ccaatggcat cactgctatc tttggcgtct ccggtgccgg
    25321 aaaaacttcg ctgattaacg ccatcagtgg actgacgcgc ccgcaaaaag ggaggattgt
    25381 cctcaatggt cgggtactaa atgatgccga aaaaggtatc tgcctgacgc cagaaaagcg
    25441 tcgcgttggc tatgtttttc aggatgcgcg gctgtttccg cattacaaag tgcgtggcaa
    25501 tctgcgctac ggcatggcga aaagcatggt cgatcagttc gataagctgg tggcgctttt
    25561 aggcattgaa ccgttgcttg accgtttacc aggcagcctg tccggaggcg aaaaacagcg
    25621 cgtagcgatt gggcgggctt tgctgacggc accagaattg ctgttgctgg atgaaccgct
    25681 ggcgtcactg gatattccgc gtaaacgcga actgttgcct tatctgcaac gtctgacgcg
    25741 ggaaatcaac atcccgatgt tgtatgtcag ccattcgctg gatgagatcc tccatttggc
    25801 agacagagtg atggtactgg aaaacggtca ggtgaaagcc tttggcgcac tggaagaagt
    25861 ctggggtagt agcgtgatga atccgtggct gccgaaagag caacaaagta gcattctgaa
    25921 agtgacggtg cttgagcatc atccgcatta cgcgatgacc gcgctggcgc tgggcgatca
    25981 gcatttgtgg gtcaataagc tggacgaacc gctgcaagct gcgctacgta tccgcattca
    26041 ggcttccgat gtttctctgg ttttacaacc gccgcagcaa accagcattc gtaacgtatt
    26101 gcgggcaaaa gttgttaata gttatgacga caacggccag gtggaagtgg agctggaagt
    26161 cggcggtaaa acgctgtggg cgcgtatcag cccgtgggcc agggatgaac tggcgatcaa
    26221 acctggcctg tggctgtacg cgcaaattaa aagtgtgtcg ataaccgcct gattaaatca
    26281 ggtggctata aatgaactgg gcaatgctgt cggtggtgtt atcaccaatc acaatgttgg
    26341 cgcgcgcttt taccgcgtca tcggcgttgc ccatcgccac gcctgtacca gcggcttcca
    26401 gcatactgat atcattaaag ttatcgccga atgccacgac gttttccatc gaccaacctt
    26461 gcgcctcaac ccatttcgtc aaacgtttac ctttgctgtt gccgccgcgt gcaatatcaa
    26521 cctgatcgtg ccaggaccat tcacactcca gtcccagttc atgttcgaca tgcttaccaa
    26581 aatgctgcaa ttgcggcagg tcatcgtgcg tcagggcgaa cttccatacg gcgttaactt
    26641 gttgcgccgt ttcagccaga gaagcgactt gtgtgaaagt cggacgctgt tccggcggca
    26701 gggtttgcgc ccagttagac gtgcgaatga catgcccggt cgggtgctca tagaccattg
    26761 catcatcgac atacatcaga ccgtgaatgt ggtgttcatt cagcatctca atgagttgca
    26821 gggctttatt aacgggcatt gggtccgctt ccagcacggt ttttgcatga taatcataca
    26881 aataggtgcc attacagcaa atagcaggtg tatccagcgc cagcgcctga taaaaaggat
    26941 gaatagcgac gtgatggcga cctgtgacga tgattaattg atagcctgct tcgcgagcgc
    27001 gggccagggc ttctatcgat gaaggaagca gggtcttttt cggggtcaat aaggtgccgt
    27061 ctaagtcgag agcaatcacg cgtgtggtca tggcgtattc cagattaagg ttaagaattt
    27121 tcgtctgcgc gaatggtaca ccgataccac tatcggacaa aattctgcgt tttaattcag
    27181 cattcaccgc caaaagcgac taattttagc tgttacagtc agttgctaaa tgcaaaggag
    27241 cattcatgaa gcaaacagtt tatatcgcca gccctgagag ccagcaaatt cacgtctgga
    27301 acctgaatca tgaaggcgca ctgacgctga cacaggttgt cgatgtgccg gggcaggtgc
    27361 agccgatggt ggtcagcccg gacaaacgtt atctctatgt tggtgttcgc cctgagtttc
    27421 gcgtcctggc gtatcgtatt gccccggacg atggcgcact gacctttgcc gcagagtctg
    27481 cgctgccggg tagtccgacg catatttcca ccgatcacca ggggcagttt gtctttgtag
    27541 gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga agatggcctg ccagtgggcg
    27601 tcgtcgatgt ggtcgagggg ctggacggtt gccattccgc caatatctca ccggacaacc
    27661 gtacgctgtg ggttccggca ttaaagcagg atcgcatttg cctgtttacg gtcagcgatg
    27721 atggtcatct cgtggcgcag gaccctgcgg aagtgaccac cgttgaaggg gccggcccgc
    27781 gtcatatggt attccatcca aacgaacaat atgcgtattg cgtcaatgag ttaaacagct
    27841 cagtggatgt ctgggaactg aaagatccgc acggtaatat cgaatgtgtc cagacgctgg
    27901 atatgatgcc ggaaaacttc tccgacaccc gttgggcggc tgatattcat atcaccccgg
    27961 atggtcgcca tttatacgcc tgcgaccgta ccgccagcct gattaccgtt ttcagcgttt
    28021 cggaagatgg cagcgtgttg agtaaagaag gcttccagcc aacggaaacc cagccgcgcg
    28081 gcttcaatgt tgatcacagc ggcaagtatc tgattgccgc cgggcaaaaa tctcaccaca
    28141 tctcggtata cgaaattgtt ggcgagcagg gactactgca tgaaaaaggc cgttatgcgg
    28201 tcgggcaggg accaatgtgg gtggtggtta acgcacacta accgctgatt taaccggcgc
    28261 agtctctcct gcgccggtgt attaacctat ctcctgtaac gcatgtctct ggcgttcgac
    28321 gatattggtc cacaaattgt ctttatcgtc agtccacaaa ttaatcagca aggcaaaaaa
    28381 gcgttcagct gccggagaaa gcacggcatc tttgcggcga ataatcccca atgtccgacg
    28441 tataacgggt tccaccagtg ggataccaat aatcgaagaa tagggcgcat ggggcattgc
    28501 cagaccagga agcgccgaaa tccccagtcc tgcctccact agtcctaatg acgtcgaaag
    28561 atgacgcact tcgtaaaacc agtccagctt ccagggcttg tcggctagct gttgttctat
    28621 caacaatcgg ttgccgctgg aggaacgtac gccaatcatt ttgtaaccca ctaactcctg
    28681 ccattctaca agctgctttt tggccaatgg atggtcacgc cgacaggcca gcacgaatgg
    28741 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
    28801 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
    28861 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
    28921 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
    28981 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
    29041 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
    29101 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
    29161 tttagcggca ttgttaaatg aactggactc tgccagtatg acaaatgcct tcatacttga
    29221 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggtcgt
    29281 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
    29341 gctaaagtca ttagagatgc tttgtcctta atgtaaccat atcgcaataa gttatgtttt
    29401 taaattgagg gcattattat gaaaaaaata ccctgcgtga tgatgcgagg tggaacctcg
    29461 aggggcgcgt tcttgttagc ggaacattta cccgaagatc aaacgcagcg cgataaaata
    29521 ttgatggcaa ttatgggttc cggtaacgat ctggaaattg acggtattgg cggcggtaat
    29581 ccgctgacca gtaaagtcgc cattattagc cgttccagcg atccgcgtgc tgatgtcgat
    29641 tatctgtttg ctcaagttat tgtccatgag caacgtgtcg ataccacgcc taattgtggc
    29701 aatatgctgt ctggtgttgg ggcattcgcc attgaaaatg gtttgattgc agcgacatca
    29761 ccggtcaccc gtgtgcgtat ccgcaacgtc aatacgggta cgttcatcga agctgatgtg
    29821 caaacgccaa atggtgttgt cgagtacgag ggtagtgcca gaattgacgg cgtaccgggt
    29881 actgccgcac cggttgcgct cactttcctg aatgccgctg gaaccaaaac cggaaaagtt
    29941 ttcccgactg ataatcagat tgattatttt gacgatgtcc cggtgacctg tatcgatatg
    30001 gcgatgccag tcgtcattat tccggctgaa tatctgggaa aaacaggtta tgaattaccg
    30061 gcggaactgg atgccgacaa agcattatta gcccgcattg aatctatccg tctacaagcg
    30121 ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta tccctaaacc tgtgcttatt
    30181 tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt ttatgccgca ttcttgccat
    30241 cgcgcgctgg cgataaccgg tgctattgct atttccagta gttgtgcatt ggaaggcacc
    30301 gtcacccgac aaatcgtccc ttctgtagga tacggcaata tcaatattga acaccccagt
    30361 ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg ccacgacgtt acgcgcatct
    30421 gttattcgga cgaccagaaa aatattttcc ggtgaagttt atcttccctg aaaaaattcg
    30481 ttgtcaagat aaggataatc aataaaggac ttctgtatga gtcatacaga aagaacagga
    30541 ttttaaatga ataagaaatc gttatggaag ctaattctga tattagcgat cccatgtatt
    30601 attggtttta tgccagctcc ggcaggatta agcgaactgg cgtgggtgct ttttggtatt
    30661 tacctggcgg ccattgtggg gctggttatc aagcctttcc cggaacctgt cgtactgtta
    30721 attgccgttg ctgcctccat ggtggtggtc ggtaacttat ccgacggtgc gtttaagacc
    30781 accgccgtat taagcggtta ctcttcaggt actacctggc tggtgttctc ggcgtttacc
    30841 ttaagcgccg catttgtgac caccggttta ggtaaacgta ttgcctatct gctgattggt
    30901 aaaatcggta acaccacgct gggtctgggt tacgttacgg tattcctcga tctggtactg
    30961 gctccggcaa caccgtctaa caccgcgcgt gcgggcggta ttgtgttacc gatcatcaac
    31021 agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc cgcgtcgtgt cggacattac
    31081 ctgatgatgt ccatttacat ggtcaccaaa accaccagct atatgttctt taccgcaatg
    31141 gcggggaaca ttctggcgct gaaaatgatc aacgacattc tgcacctgca aattagctgg
    31201 ggtggatggg cactggccgc cggattgccg ggcatcatta tgctgctggt caccccactg
    31261 gtgatttata ccatgtatcc gccagaaatt aagaaggtgg ataataaaac catcgctaaa
    31321 gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct cggtgtcttt
    31381 gtgctggcgc tactgggctg gattttcagt aagtctctgg gggttgatga atccactgtg
    31441 gcaatcgttg ttatggcgac catgctgctg ctgggtatcg ttacctggga agacgtggtt
    31501 aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg attaagctcc
    31561 ttattatcga aagttaaatt cttcgaatgg ttagctgaag tctttaaaaa taacctggca
    31621 tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat tatcgtgcgt
    31681 tatttcttcg cttccggtag tgcctatatc gttgctatgt taccggtatt tgccatgctg
    31741 gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt ctccaactcc
    31801 tatggcggca tggttactca ctatggcggc gcggcaggtc cggtcatctt tggcgtgggt
    31861 tataacgata ttaaatcctg gtggttggtc ggtgcggtac tgacgatatt aaccttcctg
    31921 gtgcatatca ccctcggcgt gtggtggtgg aatatgctga tcggctggaa catgctgtaa
    31981 atattctcgt catacttcaa gttgcatgtg ctgcgtctgc gttcgctcac cccagtcact
    32041 tacttatgta agctcctggg gattcactcg cttgtcgcct tcctgcaact cgaattattt
    32101 agagtatatc catttattat ctttctgcgc acttcacggt gcgcagatat ctggagcatt
    32161 tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag taataacgaa ataattgccg
    32221 aagaacattt caccggcgaa attaaaaaag aagaagccaa aaaaggcact attgcctggt
    32281 ctattctctc ttcgcataat acgtccggaa atatggataa acttaaaatt aagtttgatt
    32341 cattagcctc tcacgatatt acctttgttg gtattgtaca gaccgctaaa gcgtccggaa
    32401 tggaacgttt cccgctgccg tatgtgctga ccaactgcca taactcactc tgcgccgtcg
    32461 gcggcaccat taacggtgat gaccatgttt ttggtttatc ggcggcccag cgttatggcg
    32521 gtatttttgt gcctccgcac attgcggtca tccatcaata tatgcgtgag atgatggcag
    32581 gcggcggcaa aatgatcctc gggtcagaca gccacacccg ttacggtgca ttagggacaa
    32641 tggcagtcgg tgagggtggc ggtgagttgg taaaacagct gcttaatgac acctgggata
    32701 tcgactatcc gggcgtggtt gcggtgcacc tgaccggaaa acctgcgccg tatgtggggc
    32761 cacaggatgt ggcgctggct atcattggcg cggtgttcaa aaacggttac gtcaaaaaca
    32821 aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc taccgatttc cgtaacagcg
    32881 ttgacgtgat gaccacggaa accacctgtt taagctccgt ctggcaaact gatgaagaag
    32941 tgcataactg gctggcgctg cacggtcgcg gccaggatta ctgccagctt aaccctcaac
    33001 cgatggcgta ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg
    33061 cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac actgaaccag aacctgaccg
    33121 acattctgcg tgagattgaa attgagtccg aacgcgtggc gcacggtaaa gccaaactct
    33181 cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct
    33241 gttctggcgg taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct
    33301 gtggcaatga caccttctcg ctggcagttt acccgtcatc acagccggtg tttatggatc
    33361 tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg ggcaatcatc agaaccgcgt
    33421 tctgcggccc atgctttggc gcgggcgata cgccaatcaa caacggtttg agtattcgcc
    33481 acaccacgcg taacttcccg aaccgcgaag gctctaagcc agctaatggg cagatgtcag
    33541 cggtggcgtt gatggacgct cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa
    33601 cctctgccag cgaacttgat tgctgggaca acgtgccgga gtacgccttc gatgtaacgc
    33661 cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc aactcagcaa ccgctgattt
    33721 acggaccgaa cattaaagac tggccggaat tgggtgcgct gactgacaat atcgtcctga
    33781 aagtgtgctc gaagatcctc gacgaagtga ccaccaccga cgaactgatt ccttccggtg
    33841 aaacctcttc ttatcgttca aatccgattg gtctggcgga gtttaccctg tcacgccgcg
    33901 atcccggtta tgttggcaga agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg
    33961 ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc
    34021 atattgatcc gctgcaaact gaaattggca gcatggtata tgcggtgaaa ccaggcgatg
    34081 gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg
    34141 ccgaagagta cgcgactaaa cgctaccgtt ctaacgtcat caactggggg atgttaccgc
    34201 tgcagatggc ggaagtgcca acctttgaag tgggggatta catttacatc cctggcatta
    34261 aagcggcgct ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc
    34321 cggtaacgga aattacgctc tatatggaaa gtctgactgc tgaagagcgc gagattatca
    34381 aggcgggtag tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat
    34441 gtaggtcgca ttcggcactt attgtcggat gcgatgcttg cgcatcttat ccgacctacg
    34501 aatcgcatcg aatctgtagg ccagataagg cattttcgca gcatccggca cttattgtcg
    34561 gatgcgatgc ttgcgcatct tatccggcct acaaatcgca tcgaaccgta ggccggataa
    34621 ggcgtttacg ccgcatccgg caaatagtta attgctctta cttcttcgcc tctgcaacca
    34681 ctttactacc cacgccgcgg ttattgtatt cccacatgcg gttgtagtta gtgtcattca
    34741 gattgcgctg tatttcgtcg ttatcatcta cgctgccggt attacccgca aacggacgat
    34801 tagagatcac cgcatcggcc cacggtttag ccgtgttaaa accttcgttg atggcgctat
    34861 cacggatcac cacctgaccg ttggtattgg catcaacatc cagcgagcgg cccagttgcg
    34921 ccacaccatc accgaaagca ttgaaacggc tgtttacggc gaggaaaccg tagtaaatgt
    34981 tggacagcgt agccggtgca aacacatacg cttcttgctg agtacgtgag ttcaccacgc
    35041 ggaattcggt gttatcgaac accactgcgc cgcgaccaga aacgatatcc acatcccctt
    35101 caatgtagct gttggtcacc agcgtacgcg gctgacggtt ggtttccaga cggttctgca
    35161 caccgctgtt ggtgacaaag aaggtgttct gacgaccgag aatgttaacg ttgttaatct
    35221 gtacctggtc accatcagta cgcagtgcca ccgccggatg gttacctgca tctacgctat
    35281 cgcccagcgt gttttcgatg gtcagatttt gcagttgcag gccattgttt tgtgaccaga
    35341 agaccgcaga gcagagaaca ccgatactgt cgctgcgttt gctctggcag ctatcgtaca
    35401 tataccacgc tggtttacct ggcatatatt tgccgcgcgg gttgacgtcg tgacgccagt
    35461 cggcagggct catgccacca tcaagggaaa gcccaatctt cacatcaatc ggtttttcac
    35521 ctgtaccgta cagagtaatt ccacccggag cggcagggac atataccgtt ccctgatact
    35581 caccaggcat cacggcaata tactggcgct tgttggtacg cttgataatt gccgcatcta
    35641 ccgccgcctg aatcgtggta tgcgttacac cttgagtgcc cgccgggccg acaacaaagt
    35701 caggttgcgc aggcagggta atcggggaag gattccacgc tgcagcacct ggtgtcaggg
    35761 atgcaaaata gtgttgagca tcgaaattct gcgcttcttt tgccgacaga atcgggcgag
    35821 aagaggtacc aggcgcggtt tgatcagaag gacgttgatc gggcggggtt gagctacagg
    35881 cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg ggaaactgaa aatgtgttca
    35941 caggttgctc cgggctatga aatagaaaaa tgaatccgtt gaagcctgct tttttatact
    36001 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
    36061 gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccctgcctc tgtcatcacg
    36121 atactgtgat gccatggtgt ccgacttatg cccgagaaga tgttgagcaa acttatcgct
    36181 tatctgcttc tcatagagtc ttgcagacaa actgcgcaac tcgtgaaagg taggcggatc
    36241 cccttcgaag gaaagacctg atgcttttcg tgcgcgcata aaataccttg atactgtgcc
    36301 ggatgaaagc ggttcgcgac gagtagatgc aattatggtt tctccgccaa gaatctcttt
    36361 gcatttatca agtgtttcct tcattgatat tccgagagca tcaacatgca atgctgttgg
    36421 gatggcaatt tttacgcctg ttttgctttg ctcgacataa agatatccat ctacgatatc
    36481 agaccacttc atttcgcata aatcaccaac tcgttgcccg gtaacaacag ccagttccat
    36541 tgcaagtctg agccaacatg gtgatgattc tgctgcttga taaattttca ggtattcgtc
    36601 agccgtaagt cttgatctcc ttacctctga ttttgctgcg cgagtggcag cgacagggtt
    36661 tgttgttata tggccttcag ctattgcctc tcggaatgca tcgctcagtg ttgatctgat
    36721 taacttggct gacgccgcct tgccctcgtc tatgtatcca ttgagcattg ccgcaatttc
    36781 ttttgtggtg atgtcttcaa gtggagcatc aggcagaccc ctccttattg ctttaatttt
    36841 gctcatgtaa tttatgagtg tcttctgctt gattcctctg ctggccagga ttttttcgta
    36901 gcgatcaagc catgaatgta acgtaacgga attatcactg ttgattctcg ctgtcagagg
    36961 cttgtgtttg tgtcctgaaa ataactcaat gttggcctgt atggcttcag tgattgcgat
    37021 tcgcctgtct ctgcctaatc caaactcttt acccgtcctt gggtccctgt agcagtaata
    37081 tccattgttt cttatataaa ggttaggggg taaatcccgg cgctcatgac ttcgccttct
    37141 tcccatttct gatcctcttc aaaaggctac ctgttactgg tcgatttaag tcaaccttta
    37201 ccgctgattc gtggaacaga tactctcttc catccttaac cggaggaggg aatatcctgc
    37261 actcgcgtac ccatcgacga actgtttcaa ggcttcttgg gcgtcgctgg cgtgcgttcc
    37321 actcctgaag tgtcaagtac atcgcaaagt ctccgcaatt acacgcaaga aaaaaccgcc
    37381 atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttacg tctgcatgtg
    37441 ctatctgcgc ccacagcatc cagtggtcat agcagtcgct gatgttctcg gcttcgataa
    37501 ctctgttgaa tggttctcca ttccattcac ctgtgactcg gaagtgcatt tatcatctcc
    37561 ataaaacaaa acccgccgta gcgagttcag ataaaagaaa tcctcgtcag tgcgaggatg
    37621 ctgttcattg ctgctataca cttttttgct ctcaacgtaa gcggtagccc attctgttgg
    37681 gttggtgcag ttgcttttag gaaatgctat ttacccctta aatttcggct gaaagagcta
    37741 aaatccatgc aaaaaattta cgcaattttg tgtattattg tgcagtaagt aatgagctat
    37801 tttctgcgca aaaaatggat ggtaaatttg tccgggtcag gaaaaatttt atgggcgcta
    37861 aacatgaaaa aagattcgta tccttatttg atttgcatga cagtttcagg gctgatcttt
    37921 attttccttt tcttctggtg gcgggcagat atctacaggg tcacgtttct taatcagagt
    37981 atatcccact attacattct gtttagcatg ggaatagctt ttctgttatc tctgttttgg
    38041 gttaagaagg ggatagtaaa acaaagaggc tggaagagtc tgtcagcata ccttaaggtt
    38101 tatgcaggga tgtgcatatt tgctggattt tttctgatta taccccttac gacactaact
    38161 tattttttgc ctggagagac atcgtcttat gttgcaccgt atcggtatac ttccggtagt
    38221 tcaaaaagtt gttctggagc tgaggtggat gaccccgaac tacatgagaa tattcgcatt
    38281 tgctatccgt atggcaatta tgagtacgat aatattatct atgttgaaaa gaaaattaat
    38341 atattaggtg cggtagtgac atatgcacag a
//
LOCUS       DAMZII010000033        38283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZII010000033 DAMZII010000000
VERSION     DAMZII010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.033"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            124..1782
                     /locus_tag="POA77_001646"
     CDS             124..1782
                     /locus_tag="POA77_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDI6365096.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1779..2735)
                     /locus_tag="POA77_001647"
     CDS             complement(1779..2735)
                     /locus_tag="POA77_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDI6365097.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2886..4001
                     /gene="macA"
                     /locus_tag="POA77_001648"
     CDS             2886..4001
                     /gene="macA"
                     /locus_tag="POA77_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDI6365098.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3998..5944
                     /gene="macB"
                     /locus_tag="POA77_001649"
     CDS             3998..5944
                     /gene="macB"
                     /locus_tag="POA77_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDI6365099.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6017..6241)
                     /gene="cspD"
                     /locus_tag="POA77_001650"
     CDS             complement(6017..6241)
                     /gene="cspD"
                     /locus_tag="POA77_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDI6365100.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6564..6884
                     /gene="clpS"
                     /locus_tag="POA77_001651"
     CDS             6564..6884
                     /gene="clpS"
                     /locus_tag="POA77_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDI6365101.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6915..9191
                     /gene="clpA"
                     /locus_tag="POA77_001652"
     CDS             6915..9191
                     /gene="clpA"
                     /locus_tag="POA77_001652"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDI6365102.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9535..9622)
                     /locus_tag="POA77_001653"
     tRNA            complement(9535..9622)
                     /locus_tag="POA77_001653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9586..9588),aa:Ser,seq:gga)
     gene            complement(9876..10094)
                     /gene="infA"
                     /locus_tag="POA77_001654"
     CDS             complement(9876..10094)
                     /gene="infA"
                     /locus_tag="POA77_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI6365103.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10379..11083)
                     /gene="aat"
                     /locus_tag="POA77_001655"
     CDS             complement(10379..11083)
                     /gene="aat"
                     /locus_tag="POA77_001655"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDI6365104.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11125..12846)
                     /gene="cydC"
                     /locus_tag="POA77_001656"
     CDS             complement(11125..12846)
                     /gene="cydC"
                     /locus_tag="POA77_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDI6365105.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12847..14613)
                     /gene="cydD"
                     /locus_tag="POA77_001657"
     CDS             complement(12847..14613)
                     /gene="cydD"
                     /locus_tag="POA77_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDI6365106.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLAEWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14736..15701)
                     /gene="trxB"
                     /locus_tag="POA77_001658"
     CDS             complement(14736..15701)
                     /gene="trxB"
                     /locus_tag="POA77_001658"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI6365107.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16246..16740
                     /gene="lrp"
                     /locus_tag="POA77_001659"
     CDS             16246..16740
                     /gene="lrp"
                     /locus_tag="POA77_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDI6365108.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16875..20981
                     /gene="ftsK"
                     /locus_tag="POA77_001660"
     CDS             16875..20981
                     /gene="ftsK"
                     /locus_tag="POA77_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6365109.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPV
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVIIRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21136..21747
                     /gene="lolA"
                     /locus_tag="POA77_001661"
     CDS             21136..21747
                     /gene="lolA"
                     /locus_tag="POA77_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDI6365110.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21758..23101
                     /gene="rarA"
                     /locus_tag="POA77_001662"
                     /gene_synonym="mgsA"
     CDS             21758..23101
                     /gene="rarA"
                     /locus_tag="POA77_001662"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDI6365111.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23192..24484
                     /gene="serS"
                     /locus_tag="POA77_001663"
     CDS             23192..24484
                     /gene="serS"
                     /locus_tag="POA77_001663"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6365112.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24723..27167
                     /gene="dmsA"
                     /locus_tag="POA77_001664"
     CDS             24723..27167
                     /gene="dmsA"
                     /locus_tag="POA77_001664"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDI6365113.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27178..27795
                     /gene="dmsB"
                     /locus_tag="POA77_001665"
     CDS             27178..27795
                     /gene="dmsB"
                     /locus_tag="POA77_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI6365114.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27797..28660
                     /gene="dmsC"
                     /locus_tag="POA77_001666"
     CDS             27797..28660
                     /gene="dmsC"
                     /locus_tag="POA77_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDI6365115.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28696..29322)
                     /locus_tag="POA77_001667"
     CDS             complement(28696..29322)
                     /locus_tag="POA77_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6365116.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29637..30785
                     /locus_tag="POA77_001668"
     CDS             29637..30785
                     /locus_tag="POA77_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365117.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30995..32425
                     /locus_tag="POA77_001669"
     CDS             30995..32425
                     /locus_tag="POA77_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6365118.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32426..33334)
                     /locus_tag="POA77_001670"
     CDS             complement(32426..33334)
                     /locus_tag="POA77_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6365119.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            33434..34024
                     /locus_tag="POA77_001671"
     CDS             33434..34024
                     /locus_tag="POA77_001671"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDI6365120.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNKGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(34106..34846)
                     /gene="pflA"
                     /locus_tag="POA77_001672"
     CDS             complement(34106..34846)
                     /gene="pflA"
                     /locus_tag="POA77_001672"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDI6365121.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35038..37320)
                     /gene="pflB"
                     /locus_tag="POA77_001673"
     CDS             complement(35038..37320)
                     /gene="pflB"
                     /locus_tag="POA77_001673"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6365122.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37375..38232)
                     /gene="focA"
                     /locus_tag="POA77_001674"
     CDS             complement(37375..38232)
                     /gene="focA"
                     /locus_tag="POA77_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDI6365123.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9443 a         9334 c        10180 g         9326 t
ORIGIN      
        1 cgcgattatt cgctcatttt tcagacattt gccatgctta aatgtgatgt catcacgtat
       61 tagcaaggcc tttcccgtta tactgccagc gtaaaggata agtcacatat ttctggaggg
      121 gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg tttgtcgttg
      181 atgctcgaac aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc cagcttgctg
      241 gacgccttaa ccctgctgct atcgccagaa tcagatctct accattttga gcgcgacgat
      301 ttctggttcc cgccgggaga tatcaacggg cgagaacatc atctgcatat tattttgacc
      361 ttccgcgaat cgctgccagg ccgacatcgg gttcgccgtt atcggccgct ggaagcgtgc
      421 tggacgccat gcaccgatgg ctatcaccgt attttttatc gtctggaagg ggagagtgcg
      481 gaagacggca gcgtgatgac actgcgcagt tttctcgata aagacggaca tccgattgat
      541 gtcgaggata ttaacgatca ggcacgccat ctggtgcgtt taatgccggt gctgcgcttg
      601 cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt tcccaatgtg
      661 gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt tgtcctcaca tccgcaaaat
      721 ctctctgatg gacagattcg tcagggactt tccgcaatgg tgcagttgct tgagcattat
      781 ttctctgagc agggggccgg acaggcgcga tatcgtttaa tgcggcggcg ggccagcaat
      841 gagcaacgaa gctggcgcta tctggacatc atcaaccgga tgattgaccg acctggaggg
      901 cgctcgtatc gggttatttt gctcggcctg tttgctactt tattgcaggc aaaaggcaca
      961 ttgagactgg ataaagacgc gcgtccgtta ttgctaattg aagatccaga aacccgttta
     1021 caccccatta tgctttcagt tgcctggcat ctgttgaatc ttctgccatt gcagcgcatt
     1081 gccaccacca actcgggtga gttgctttcg ttaacgccgg tagagcatgt ttgccgactg
     1141 gtacgtgagt cctcgcgcgt tgccgcctgg cgtctggggc cgagtggctt gagtaccgaa
     1201 gatagccgac gcatatcctt tcacattcgt tttaaccgtc cgtcatcgct gtttgctcgc
     1261 tgctggttgc tggtggaagg ggaaacggaa acctgggtta tcaatgaact ggcgcgccag
     1321 tgtggacacc atttcgatgc cgaagggatc aaggtcattg agtttgccca gtccgggcta
     1381 aagccactgg ttaaatttgc ccgccgaatg gggattgaat ggcatgtact ggtcgatggc
     1441 gatgaagcag ggaagaaata tgccgctacg gtacgcagcc tgttgaataa cgatcgggaa
     1501 gccgaacgag aacatttaac ggcgttaccg gcgctggata tggaacattt tatgtatcgc
     1561 cagggatttt ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatgt accgatgaat
     1621 ctgcgcaaaa ttatctcgaa agcgatccat cgctcttcca aacccgatct tgccattgaa
     1681 gtggcaatgg aggccggacg tcgtggtgtg gactccgtac cgacgctgct gaaaaaaatg
     1741 ttctcacgcg tgctgtggct ggcgcgcggt cgcgcggatt aaccgcgaaa catcgtggcc
     1801 atttgtggct gaatagcgtc gagcatctca tagcgccgac ggtattcagc ccgtttttta
     1861 ctggcgattt cggcaatctc ttttcgtgct atctgtgctg gaaggcggta atggcgttca
     1921 gcatcacata cgccgccaac cgattcccag aaagcgttgt aatcggcatg gatcttgcct
     1981 tctttatcac gataacgcag gctgcggtaa atatgcgttt cattgctgac ggcaataatc
     2041 tgctctacct gcaaacgttg ggcaaacaga caggccgctt ccatcacgag gcgtttggga
     2101 aatagcccgt ggcaggcttt cgtcgcattc tggatttcct gatgtggaat ttcccatttt
     2161 gcgccttgca gtccgccaat aaacatcgtt cttttcccct gatattcaca cagggtaaac
     2221 gtgatctctg ccagaggaat accttcgctg ttgcgaaaca ggattgtgct gtcaccttct
     2281 ttatccattg agatcatcat ggtcagctca agcgtgaact gctcgccgtt tttgccttcc
     2341 agtttagcca gttgcagccc gggggtattc aaatataagc tgaattcttc cgccgacata
     2401 catccgcgga gtaacgcata atggtaacgt aacgcctcca gcaattgctt acggctaaga
     2461 ttcgccgcaa ggtaagggcg atgcagacgc acaggcagtc gcggttggcg cgttaacaac
     2521 acattgagat taggccagtg ggaaagttcg ttcatccact caacgcttaa acgcggcata
     2581 atcaacgagc gcagcaaaaa tttctggcga aaactacggc gatgccagaa tttacccggc
     2641 cgacactgtc cacgtgccag actaagaaaa agtgacaggc tgctgagaga ttcagatggc
     2701 gtaaaggtcc gttcagttag ctgcgacata ttcatgaaat caatggttat acatgacgtc
     2761 gatttcacca ttgcgtatct taaccaaaca tcaatagtgt gattactaac gtaaatttta
     2821 gggttttgtt gatatttcgt tgaagttaat gacccggatt ggcatatgga gtattcagaa
     2881 tatttatgaa aaagcggaaa accgtgaaga agcgttacgt tattgcgctg gtgatagtca
     2941 tcgccggact gattacgtta tggagaattc ttaacgcacc cgtgccgact tatcagacac
     3001 tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc
     3061 tgcgtaaggt tgacgtgggc gcgcaggtca gcggtcagtt gaaaactctg tcggtggcga
     3121 ttggcgataa agtaaaaaaa gaccagcttt taggggttat tgatcctgaa caggctgaaa
     3181 accagattaa ggaggtcgaa gcaacgctga tggagctacg tgcgcagcgg cagcaggcgg
     3241 aagcggagct gaaactggcg cgggtgacgt attcccgtca gcaacgtctg gcacaaacgc
     3301 aggcggtttc acagcaggat ctcgacaccg ccgcgacgga gatggctgtg aaacaggcgc
     3361 aaattggcac cattgacgcg caaatcaagc gcaatcaggc ctctctcgat acggctaaaa
     3421 ccaatctcga ttacactcgc atcgttgccc cgatggccgg ggaagtcacg caaatcacca
     3481 ctctgcaagg ccagacggtg attgccgcac aacaagcacc gaacattctg acgctggcag
     3541 atatgagcac catgctggta aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc
     3601 cggggcaaaa agcctggttt acggtacttg gcgatccact gacgcgctac gaggggcaaa
     3661 tcaaggatgt actaccgacg ccggaaaagg ttaacgacgc tattttctat tacgcccgtt
     3721 ttgaagtccc caaccccaat ggtttgctgc ggctggatat gactgcgcaa gtgcatattc
     3781 agctcaccga tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc gatccggttg
     3841 gcgataatcg ttataaagtc aaattgttgc gtaatggtga aacacgcgag cgtgaagtga
     3901 cgattggcgc acgtaacgat accgatgttg agattgtcaa agggcttgaa gcgggcgatg
     3961 aagtggtgat tggtgaggcc aaaccaggag ctgcacaatg acgcctttgc tcgaattaaa
     4021 ggatattcgt cgcagctatc ctgccggtga tgagcaggtt gaggtgctga agggcatcac
     4081 cctcgatatt tatgccggtg agatggtagc gattgttggc gcttcaggtt ccggtaaatc
     4141 gaccctgatg aatattctcg gctgtctgga taaggccacc agcggcacct atcgcgtcgc
     4201 cggtcaggat gttgccacac tggacgccga tgcgctggcg caactgcgcc gcgagcattt
     4261 cggctttatt ttccagcgtt accatttact ttcgcattta accgccgagc agaacgttga
     4321 agtacccgcc gtctatgccg gtcttgagcg gaaacagcgg ctgcttcgcg cccaggggtt
     4381 actgcaacga ctggggctgg aagaccgtac agagtattat ccggcacagc tttcgggtgg
     4441 tcagcaacag cgcgtcagca tcgcgcgggc attgatgaac ggcggtcagg taattcttgc
     4501 cgatgaacca accggcgcac tggacagcca ttctggcgaa gaggtgatgg cgatcctgca
     4561 tcagctgcgc gatcgcgggc atacggtgat tatcgtcacc cacgatccgc aggtcgctgc
     4621 tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccat
     4681 tgaaaaagtg aatgttgctg gcgggacgga gcctgttgtc aacacggtgt ctggctggcg
     4741 gcagtttgtc agcggtttta acgaggcgct gacgatggca tggcgggcgc tggcagcgaa
     4801 taaaatgcgt actttactga ccatgctggg gattattatc ggtattgcgt cggtggtttc
     4861 cattgtcgtg gtgggtgacg ccgccaaaca aatggtgctg gcggatattc gttctattgg
     4921 tacgaatact attgatgtct atcccgggaa agattttggc gatgacgatc cgcaatatca
     4981 gcaggcgctg aagtacgacg acttaatcgc catccaaaaa caaccgtggg tcgcctcagc
     5041 cacacctgcc gtctcgcaaa acctgcgcct gcgttataac aatgttgatg ttgctgccag
     5101 tgccaatggc gtgagcggcg attattttaa tgtctatggc atgaccttca gtgaaggaaa
     5161 cacctttaat caggagcagc tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac
     5221 tcgccgccag cttttccccc ataaagcaga tgtggttggc gaggtgattc tggtcggcaa
     5281 tatgcccgcc agagtcattg gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa
     5341 agtgctgcgt gtctggctac cttacagcac gatgtccggg cgagttatgg gccagtcgtg
     5401 gctgaactcc attactgtca gggtaaaaga aggatttgac agcgccgagg cggaacagca
     5461 actcacgcgt ttactttcac tgcgccacgg aaagaaggat ttctttacct ggaacatgga
     5521 cggcgtcttg aaaactgttg aaaagaccac acgtacttta caactgtttc tgacgctggt
     5581 ggcggtgatt tcgctggtgg tgggcggtat tggtgtaatg aatattatgc tggtgtcagt
     5641 gaccgagcgg acgcgggaaa ttggcattcg catggctgta ggtgcgcgag caagcgatgt
     5701 tttgcaacag ttcctgatcg aagccgtact ggtttgcctg gtcggtggcg cgttgggaat
     5761 aacactgtca ctgttaattg ctttcacctt gcagcttttc ttacccggct gggagattgg
     5821 tttttcaccg ttggcactgc tgctggcgtt tctctgctcg acggtcaccg ggattttatt
     5881 tggatggtta cccgcacgaa atgcggcacg actggatcca gtagatgctc tggcacgaga
     5941 gtaatttttg agataaaaat gccagccgat cgggctggca ttttgccttt aggatgtaca
     6001 caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca ataacactgg
     6061 cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca gcttttagcg
     6121 ttctgtaacc atccatctga atggtggaat aatgagcgaa aatatcttcg ccgccgcctt
     6181 cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta cccttttcca
     6241 tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg ggtcagagag
     6301 ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag ggcgagccgt
     6361 caagcgtttg acaggggcca gggggcaggt atgaatcaaa aatttgaagc agttaacgct
     6421 attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt atctatcatg
     6481 tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct gccgataacc
     6541 gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac tttgatcaac
     6601 tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa gtgatattag
     6661 tcaatgatga ttacactccg atggagtttg ttattgacgt gttacaaaaa ttcttttctt
     6721 atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg aaggccattt
     6781 gcggagtctt taccgccgag gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga
     6841 gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat
     6901 tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg gctttcgcca
     6961 gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg gcgctgctca
     7021 gtaacccatc tgcccgggag gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc
     7081 aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt gaagaggagc
     7141 gcgacacaca gccgacgctg agttttcagc gtgtactgca acgtgcggtc ttccatgtcc
     7201 agtcctccgg tcgcaatgag gttaccggtg caaacgttct ggtcgctatc tttagcgaac
     7261 aggagtcgca ggcggcatat ctgttgcgta aacatgaagt cagccgtctc gatgtggtga
     7321 attttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat cctggcagcc
     7381 agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc acgacgaacc
     7441 tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat tggtcgtgag aaggagctgg
     7501 agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgctg gtgggggaat
     7561 ctggtgtcgg taaaaccgcg attgcggaag gtcttgcctg gcgaattgtt cagggcgatg
     7621 tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttct ctgttagcgg
     7681 gcactaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag cagctggagc
     7741 aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt gcgggggcag
     7801 cgtctggtgg ccaggtcgat gcggctaacc tgatcaaacc gttgctctcc agcggtaaaa
     7861 ttcgcgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag aaagaccgtg
     7921 ctctggcgcg tcgcttccag aaaattgata ttactgaacc gtcgatcgaa gaaactgttc
     7981 aaatcatcaa tggcctgaaa ccgaagtatg aagcgcacca cgacgtgcgt tataccgcaa
     8041 aagcggtgcg tgcagcggta gagctggcgg tgaaatacat taacgatcgt catctgccgg
     8101 ataaagccat tgacgttatc gacgaagcgg gcgctcgcgc acgcctgatg ccggtaagca
     8161 aacgcaagaa aaccgttaat gtggcggata ttgagtccgt ggtggcccgt attgcgcgca
     8221 ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc ggcgatcgcc
     8281 tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa gccattaaga
     8341 tggcgcgtgc aggtttaggt cacgaacata aaccggttgg ttcgttcctg tttgccggcc
     8401 ctaccggggt cgggaaaaca gaggtgacgg tacagctttc gaaagcgttg ggcattgagc
     8461 tgctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt ctgattggtg
     8521 cgcctccggg atacgttggt tttgatcagg gaggtttgct gactgatgcg gtcatcaagc
     8581 atccacatgc ggtgctgttg ctggacgaaa tcgagaaagc gcaccctgac gtgttcaata
     8641 ttctgttgca ggtgatggac aacggtacgc tgaccgataa caacggacgc aaagcggact
     8701 tccgtaacgt ggtgctggtg atgaccacca acgccggggt acgtgaaact gagcgtaaat
     8761 ccattggtct tatccaccag gataacagca ccgatgcgat ggaagagatc aagaagatct
     8821 ttacaccgga attccgtaac cgtctcgaca acattatctg gtttgatcat ctgtcaaccg
     8881 acgtgatcca tcaggtggtg gataaattca tcgtcgagtt gcaggttcag ctggatcaga
     8941 aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag aaaggttacg
     9001 accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc
     9061 tcgccaacga actgctgttt ggttcgctgg tggacggcgg tcaggtcacc gtcgcgctgg
     9121 ataaagagaa aaatgagctg acttacggat tccagagtgc acaaaagcac aaggcggaag
     9181 cagcgcatta atctgattgt taggtaggtt ggtcaagtcc gtaatctcga aagaggttac
     9241 ggactttttg tttatggggt ggaggaggtt cagacccttt ttttaatgat gatggtaagt
     9301 tgttgataat tagtgctgcg ggaaggtaag gataaaaaag ggtgctgcag gagaatggga
     9361 tggttttgct ttattaacaa cgggctaaac gtgtagtatt tgagttcact gccgtacagg
     9421 cagcttagaa atcgacgggg tgcggtaaaa cctttgcgaa cgcgttcact gccgtacaga
     9481 cagataaaat gcgaaaaaaa agctcgcact ttcgtacgag ctcttcttta aatatggcgg
     9541 tgaggggggg atttgaaccc ccgatacgtt gccgtataca cactttccag gcgtgctcct
     9601 tcagccactc ggacacctca ccaaattgtt ttgctaccaa acctcatggg tggcaacggg
     9661 gcgctactat agggagttgg agtaaaacgg tcaagaagaa ttttaatgat aattattgtt
     9721 tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta cggacttata
     9781 cttccaaagt acttcataca tatcacaaaa taaaaaggcc ggttaaaccg accttttact
     9841 cgttctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa gacaatgcgg
     9901 cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt caggatgcgg
     9961 atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc gttttctaac
    10021 tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat ttcaatattg
    10081 tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc ggcaagataa
    10141 tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa caggttcggc
    10201 acattactcc gaaaacacac ggctaagccg caccaaaagc gcaacgtata agggagcggt
    10261 gagataaacg atgggcgtta cctgacgcga aaaaatcctt atcggcagcg gggtaatgag
    10321 cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc gaaaacattc
    10381 attcttgtgg tgaaaacaag catcgtggta cccagaaatt attcggcaat cgtccgaggc
    10441 gcatttgatt gagataatta aggtaatccc ggcggggaat ttcgcaggca ccaagcgatg
    10501 ctgtgtgatc gttaaggacc tggcagtcga taagcttacc gccatgaccg ataaattcct
    10561 cacagaatac cagaagcgcc gttttagacg cattttccat ccggctgaac atggactcgc
    10621 cacaaaatag cgttccctgg gccacgccgt acataccgcc gacaagctca tcttcacgcc
    10681 agacttcaat ggagtgggca tggccgagtt cgtgaaggcg atggtaggct tcgaccacgc
    10741 cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcaatg acctgaccaa
    10801 aagcgtaatt catcgtgaca cgatagggcg agcgtttatg aaatcgcttc atactacggc
    10861 tgatatgcag tgattctggc catagcaccg cgcggggatc gggcgaccac cagaggatgg
    10921 ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta agccattaac aggcgcgcag
    10981 ggctaagatc gcccccaagt gccagcaggc cgttaggctc acgtaatgcg ccttccgggg
    11041 aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca aaactccacg
    11101 caagtcggat cgttcaataa tagcttacaa accctgcttg aactggtaat aacgcccctg
    11161 tctggcaagc agctctgcgt gagtaccttg ctcaataatt tgcccgttgt ccatcactat
    11221 tatttgttgg aaacgagaga gtccgcgaag tcgatgggtg accattaaca ccgttttctc
    11281 acgcatcatt tctgcaagca attcaaggat ctggctttcg gttgtggcat ctaagccttc
    11341 ggtaggttca tccagcaaca ccagtggcgc atcatgtaac agcgcacggg cgatagccag
    11401 acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac tgttgagacc
    11461 tgcatcctcg agcagcttct ccaggccaac gcgacgcaag ctctccgcca gagcctcatc
    11521 actactgcca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa acagatgcac
    11581 tcgctgagga acaacgctga tggtctgtcg tagagccgct tcattcaggc tggctatggg
    11641 gctatcgtta agcaaaatct cgccctgttg cgggtcccaa gcgcgggtca gcagttgtaa
    11701 cagtgttgat ttgccgcatc cggttcgccc gagaatcgca atatgttccc cggcgtttac
    11761 ctgaagagaa atccctttaa gtgcctgttg ggattgctcc ggataagtga actgaacatc
    11821 ccgtaatgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga aggtgacttc
    11881 cggtttttga tccgttaaat cagtgatccg aacggcagag gcaatgactt gccccagatg
    11941 ctgaaatgca cccgttactg gtgccagtgc ttcaaacgcg gctaacgcgc agaagacaaa
    12001 cagggcaatt aacgcgccgg gttgagcatt gccgccaacg ccgccagacg ccatccacag
    12061 catcaggatc accgctaacg cgccaatgag tagcattatc gcttgcgaca atgcggtcag
    12121 ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta gttgcgtgcg
    12181 ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc aggccgtcag
    12241 ttgttggcga tactgcccgc gaagatgagt cagattttgc ccggtgcttt ttcccgcacg
    12301 ataaaacagc ggtggcatca ggaaaagcgt cagtaacata atgccgccca gcgtaaaggc
    12361 gagggtgaaa tcaaggaaac ttaacccgat tgtcaccacc ataatcacca caaaagcgcc
    12421 caccagcggc gagataacgc gcagataaag atgatcgagc gtatcaacat ccgccaccac
    12481 gcgattgagc aattcgccct gacgatagcg cgccagtccg gcaggggaga ggggcagcaa
    12541 tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt cgtgacttac
    12601 cagacgttca aaataacgcc cggcagtacg ggtgattgct gcgccacgca cgcccgcagc
    12661 gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg aggccgagag
    12721 gaaccagccg gaaagtgtca acagaccgat actggcgagc agcgtgacaa ttgccagcac
    12781 gataccaaga cttaacatcc atttatgacg tttatacagt gccagatagg gtagcaaagc
    12841 gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc caccagccac
    12901 acttaattcc gcgtaacgtc cttgctcaat aatccggcca tcctgcataa cccaaatgac
    12961 atcccactca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct ggcgcagaga
    13021 ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc tggcagcggg
    13081 ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg ccacgcgctg
    13141 cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggtgtatcaa cgccttgtgg
    13201 caggagcggt agaaactcgc tgacccaggc gttatccagc gctgcttgta attcttgttc
    13261 gctggcatca ggtcgcgcca gtagtacgtt atcccgcaat gttgctgccg gtaattgtgg
    13321 gttttgccca acccaggaga gatgtttacg ccatgattct ggtgataaat cgcgtaattc
    13381 tatcccgttg attcgtaatg atccctgata tgagagaaaa ccagaaagcg cgttcagcag
    13441 tgagctttta cctgaaccgc tgcgaccaac caacactgca cgttggcctg ctggcaaagt
    13501 aaagttcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca gatcctcggc
    13561 ctcaatggtc accggatcgg tcgatgctaa ttccacctcg ccacgctgcg gatgggcgag
    13621 cggggtttcc ataaacgttt tcagactgtc agctgcacca acagcctggg ctttagcatg
    13681 ataaaacgta ccgagatcgc gcaatggctg gaaaaactct ggcgcaagga tcagggccag
    13741 aaaacccgca gccagcgtca caccggtatc gtagtgacca aaatccagct cgccgagata
    13801 ggaaaaacca aagtagaccg ccaccagagc aattgacagc gaggtaaaaa attcgagaat
    13861 gccggaggat aaaaacgcca gccgtagcac ttccattgtc cgttggcgga aatcttccga
    13921 agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca atgtttccat
    13981 gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa agttacgccg
    14041 gttagcatcg gcagccccca tcccaaccag cgccataaac aacggaatta acggtgctgt
    14101 gcccagcaga atgagcgccg cagcccagtt agaggggaag atcgccacca caatcagcaa
    14161 cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat catgcatatc
    14221 gtcaatttgc tcgagtacca acgtcgccca gctccccgca ggtttaccct gaatccacgc
    14281 tggccctgct tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc ggatatgctg
    14341 cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgcgcgca gtacaaaggt
    14401 cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct ccataatcat
    14461 atgttgcaga attcgcgcca tgaaccaggc ttgggcaatg atcaatatgc cgcttacaaa
    14521 gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct gttttaacca
    14581 gcgggttaac tctttttgac gggatttatt cattgcacgc ttagcaggtg agttatcaga
    14641 attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata gtcgcatggt
    14701 gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc taaaccatcg
    14761 aggtagcgtt ccgcatcaag tgctgccatg cagcctgtac cggccgaagt aatggcctga
    14821 cgataaatat gatccatcac gtcacccgcg gcaaagacgc cagggatgct ggtctgggtg
    14881 gcattaccat gaatacccga ctgcactttg atgtagccgt tttccagttc cagctgccct
    14941 tcgaaaatgg cagtattcgg gctgtgaccg atagcaacaa acagaccagc aacgtcgagt
    15001 gactcgatgt tatcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac acccatttga
    15061 tcgccggtca cttcttccag cgtacggttg gtgtgcagaa tgatgttgcc gttctccact
    15121 ttatccatca gacgcttaat aaggattttt tccgcgcgga aaccgtcacg gcggtgaatc
    15181 agatgcactt ccgaagcgat gttagacaga tacagcgcct cttcaaccgc ggtattgccg
    15241 ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt tgcacaagca
    15301 gaaaccccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg tgcagaagct
    15361 ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt cagacggaac
    15421 ggacggtttt gcagatccac cttgttgata tgatcaaaaa tgatctcagt ttcaaacttg
    15481 gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt tggatcgcca
    15541 ggccagtttt ccacttccgt ggtggtggtc agttggccgc ctttttccat gccggtaatc
    15601 agcacaggtt gcaggttggc gcgcgccgcg tagacagcag cggtgtatcc cgccgggcct
    15661 gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc atagttgttg
    15721 gcagacaatg ggcaggattg tagggaattt acagacgtaa aaaaagagta tgacgatttt
    15781 gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc aaattctccc gtcattatca
    15841 cctctgctac ttaaatttcc cgctttataa gccgattaaa tgatgaataa acgcccctgt
    15901 taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa tcccctggtg
    15961 ttttgcgaaa acattcgagg aagaaaaaaa acagtattct tatatgcgca taaccatgca
    16021 tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca gacgccattc
    16081 gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag gcattgcgcg
    16141 ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac agtgatgttt
    16201 cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt agatagcaag
    16261 aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag
    16321 gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc aacgccgtgc
    16381 cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac ggcgctgctt
    16441 aacccccatt atctggatgc atcacttctg gtattcgttg agattactct gaatcgtggc
    16501 gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga aattcaggag
    16561 tgtcatttag tatctggtga tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca
    16621 gcttaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg
    16681 acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa gacgcgctaa
    16741 cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc atacagcaac
    16801 agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac
    16861 ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca ttgacaaagt
    16921 taagtagcgg ccgtcgcctt ctggaagcgt tgctgatcct tattgtcctg tttgccgtct
    16981 ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg caaacggcct
    17041 ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggctggca gatacgctgt
    17101 tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc ggttgttggt
    17161 ttgcctggcg tcatcagtcc agtgacgaat acattgatta ttttgccgtt tcgctacgca
    17221 tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc aacgctgacg
    17281 atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact acgctacaac
    17341 cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggct gcgggcctga
    17401 cgctgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc tggattttaa
    17461 acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg
    17521 aatatgaaga tgacgaagag tatgaagatg aaaaccacgg caaacagcat gaatcacgcc
    17581 gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg gttggcggaa aaattcatta
    17641 atccgatggg gcggcaaaca gacgctgcgt tgttctccgg taagcggatg gatgatgacg
    17701 aagagattat ctacactgca cgcggtgtgg ctgccgaccc ggacgacgtc ctattttcgg
    17761 gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaac ggtgcgccaa
    17821 ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc gacacaaagc tgggctgcgc
    17881 cggttgaacc tgtgactcag acgccgcctg ttgcctctgt tgatgttcca ccttcgcaac
    17941 ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtt attgctcctg
    18001 caccggaagg ttacccacag cagtcacaat atgcgcagcc tgcagtgcaa tataatgagc
    18061 cgctgcaaca accagtacag ccgcagcagc cgtattatgc acctgcagct gaacaacctg
    18121 cgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcaaccg tattacgcaa
    18181 ctgcgccgga acagcctgca caacagccgt attatgcccc tgtgccagaa caaccggtgg
    18241 caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc
    18301 agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa cagccgcaac
    18361 ctgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca aaacccgcgc
    18421 gtccgccgct ttactacttt gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac
    18481 ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg atcaaatctt
    18541 cgctgaaagc accttctgtt gcagcagtac ctccagtaga agccgctgcc gctgtttctc
    18601 cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg
    18661 cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa gaggggattg
    18721 gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgccgtgaa ttggcgtctt
    18781 acggtattaa gctgccctca cagcgtgcgg cggaagaaaa agcccgtgaa gcccagcgca
    18841 atcagtatga ttctggcgat cagtacaacg atgatgaaat cgatgcgatg cagcaggatg
    18901 aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag tatcaacatg
    18961 atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt
    19021 ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg aatccgttct
    19081 cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac
    19141 cgttgtttac gccaattgtt gaacctgtac agcagccgca acagccggtt gcgccacagc
    19201 agcaatatca gcagccgcaa cagccggttg cgccgcagcc gcagtatcag cagccacaac
    19261 agcaggttgc gccgcagcca caatatcagc agccgcaaca accggttgcg ccacagccgc
    19321 agtatcagca gccgcaacaa ccggttgcgc cgcagccgca gtatcagcag ccgcaacaac
    19381 cggttgcgcc gcagcagcag tatcagcagc cacaacagcc agttgcgcca caaccgcagg
    19441 ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg cataaaccga
    19501 cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg gagccggtag
    19561 atacctttgc gcttgaacaa atggctcgcc tggtggaagc gcgtctggct gatttccgta
    19621 ttaaagccga tgttgtcaat tactctccgg ggccggttat cattcgcttt gaattgaacc
    19681 tggcaccggg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt gcccgttcac
    19741 tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat gtaggtctgg
    19801 agttaccgaa taaaaaacga caaaccgttt atctgcgcga agttttggat aacgccaaat
    19861 tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc ggtgagccgg
    19921 tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc ggttccggta
    19981 aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag ccagaagatg
    20041 tacgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa ggcattccgc
    20101 atctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg cgctggtgtg
    20161 ttaacgagat ggagcgtcgt tataaactga tgtctgcgct gggtgtgcgt aatctggcgg
    20221 gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca gacccgtact
    20281 ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa ccatacattg
    20341 tggtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa gtggaagagc
    20401 tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta ctggcaactc
    20461 agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg acccgtatcg
    20521 cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct ggcgcggaat
    20581 cactgctggg gatgggggat atgctctact ctgggccgaa ctccacgttg ccggtacgtg
    20641 tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac tggaaagcgc
    20701 gtggtcgccc acagtatgtt gatggcatca cttccgacag tgaaagcgaa ggtggcgcgg
    20761 gcggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg cagtttgtca
    20821 ctgaaaaacg caaagcgtca atttctggcg tacagcgtca gttccgcatt ggttataacc
    20881 gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa caggggcaca
    20941 acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat tgccggataa
    21001 ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt tcttctttcc
    21061 tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg gagtgacgta
    21121 atttgaggaa taatgatgaa aaaaattgcc atcacctgtg cattactctc aagcttagta
    21181 gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga taaagtcagc
    21241 agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa
    21301 ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat gacacaacct
    21361 gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc gttcgttgag
    21421 caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat gctgattgcc
    21481 cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga tgactttgtc
    21541 ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt gggacgtgat
    21601 ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag ttatcaactg
    21661 aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc gccgcaaggc
    21721 gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt cgctcgattt
    21781 ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt tagcacagta
    21841 tatcggccag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta tcgaagccgg
    21901 gcatttacat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa ccctcgctga
    21961 agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca cctctggcgt
    22021 gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac cgcaatgcag gtcgccgcac
    22081 tattcttttt gttgacgaag tgcaccgatt caacaaaagc cagcaggatg catttctgcc
    22141 acatattgaa gacggcacta tcacttttat tggcgcaacc actgaaaacc cgtcgtttga
    22201 gcttaattcg gcactgcttt cccgtgcccg tgtctatctg ttgaaatccc tgggtacaga
    22261 ggatattgag caagtactaa ctcaggcgat ggaagacaag acccgtggtt atggtggtca
    22321 ggatattgtt ctgccagatg aaacacgacg cgccattgct gaactggtga atggcgacgc
    22381 gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtca atgatagcgg
    22441 taagcgggtc ctgaagcctg aattattgac cgaaatcgcc ggtgagcgaa gcgcccgctt
    22501 tgataacaaa ggcgatcgct tttacgatct gatttccgca ctgcataagt cggtacgtgg
    22561 tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt attaccgccg gtggcgatcc
    22621 gttatatgtc gcgcgtcgct gtctggcgat agcgtctgaa gatgtgggta atgctgatcc
    22681 acgagcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg gcccggcgga
    22741 aggtgaacgc gccattgctc aggcgattgt ttacctggcc tgcgcgccaa aaagcaacgc
    22801 tgtctacact gcgtttaaag ccgcgctggc ggatgctcgc gaacgtccgg attatgacgt
    22861 gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct acgggcagga
    22921 atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt acttcccgcc
    22981 ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag gcaagattgg
    23041 cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac gctaccgtta
    23101 atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg tggccacatc
    23161 tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc tgcgtaatga
    23221 gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg tagataagct
    23281 gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc tgcaagcgga
    23341 gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag atatcgagcc
    23401 tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag ccgagctgga
    23461 tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc ctgcagatga
    23521 agtgccggta ggtaaagacg aaaatgacaa cgtcgaagtc agccgctggg gcaccccgcg
    23581 tgagtttgac tttgaagttc gtgaccatgt gacgctgggt gaaatgcact ctggcctcga
    23641 ctttgcagcc gcagttaagc tgaccggttc ccgctttgtg gtaatgaaag ggcagattgc
    23701 tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac agcatggcta
    23761 cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta cgggtcagct
    23821 gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag cagacaccag
    23881 taactatgcg ctgatcccaa cggcagaagt tccgctgacc aacctggtac gcggtgaaat
    23941 cattgatgaa gatgatctgc caattaagat gaccgcccac accccatgct tccgttctga
    24001 agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt tcgacaaagt
    24061 tgaaatggtg cagatcgtgc gcccggaaga ctcaatggcg gcgctggaag agatgactgg
    24121 tcatgcagaa aaagtcctgc aactgctggg cctaccgtac cgtaaaatca tcctttgtac
    24181 cggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga tcccggcaca
    24241 gaacacctac cgcgagatct cttcctgctc caacgtttgg gatttccagg cgcgtcgtat
    24301 gcaggctcgt tgccgcagca agtcggacaa gaaaacccgt ctggttcata ccctgaacgg
    24361 ttctggtctg gctgttggtc gtacgctggt tgcagtaatg gaaaactatc agcaggctga
    24421 tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg aatatattgg
    24481 ctaataccca atttttctga atctaaaaag cgcctgcggg cgcttttttt gtctcccttt
    24541 gataccgaac aataattact cctcacttac acgtaatact actttcgagt gaaaatctac
    24601 ctatctcttt gattttcaaa ttattcgatg tatacaagcc tatatagcga gctgctatag
    24661 aaataattac acaatacggt ttgttactgg aatcaatcgt gagcaagctt gagtgagcca
    24721 ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggtgagtcgc cgtggtttgg
    24781 taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca ttacctttta
    24841 gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa aaggttatct
    24901 ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg
    24961 gtgaaatcaa atatgtcgaa acggacaata ccggcgatga caattacgac ggcctgcacc
    25021 aggttcgcgc ctgtctgcgt ggacgttcca tgcgtcgccg tgtctataat ccggaccgcc
    25081 tgaaatatcc gatgaaacga gtcggggcgc gcggtgaagg caaatttgag cgcattagct
    25141 gggaagaagc ctacgacatc atcgcgacca atatgcagcg cctgatcaaa gagtacggca
    25201 acgaatccat ctatctgaac tatggcaccg gtacgctggg cggcaccatg acccgctcct
    25261 ggcccccggg aaataccctg gtcgcgcgcc tgatgaactg ctgcggcggt tatctgaacc
    25321 attacggtga ctactcttcc gcgcaaattg cggaaggttt gaactatacc tacggcggct
    25381 gggcagatgg taacagcccg tcggatatcg aaaacagtaa actggtagtg ctgtttggta
    25441 ataaccctgg cgaaacgcga atgagtggcg gtggggtgac ttactatctt gaacaggcac
    25501 gccagaaatc taatgcccgc atgatcatca tcgatccgcg ctataccgac accggtgccg
    25561 ggcgcgaaga tgagtggatc cctattcgtc cgggaacaga tgccgcactg gttaacggtc
    25621 tggcgtacgt catgatcact gaaaacctgg tggatcaggc attcctcgat aaatattgcg
    25681 ttggctacga tgagaaaacc ctgccagcca gtgcgccgaa aaatggccac tataaagctt
    25741 atattctggg tgaagggcca gatggcgtgg ctaaaacacc ggaatgggcc tcacaaatca
    25801 ccggtgttcc ggcagacaaa atcatcaaac tggctcgtga aatcggcagt accaaaccgg
    25861 cgtttatcag ccagggatgg ggcccgcagc gtcacgctaa cggtgaaatc gcaacccgtg
    25921 ctatctcgat gctggcgatt ctgaccggta acgttggtat taacggaggc aacagcggcg
    25981 cgcgtgaagg ttcatacagc ttaccgtttg tccgtatgcc gaccttggaa aacccgatcc
    26041 agaccagcat ttcgatgttt atgtggaccg atgccattga acgtggcccg gaaatgacgg
    26101 cgctgcgtga tggtgtacgc gggaaagata agctggatgt gccgatcaaa atgatctgga
    26161 actatgccgg taactgcctg attaaccagc attctgaaat caaccgtacc catgaaatcc
    26221 ttcaggatga taagaagtgc gagctgattg tggttatcga ctgccacatg acctcatcgg
    26281 cgaaatatgc tgacatcctg ctgcctgact gcaccgcttc cgaacagatg gactttgcgc
    26341 tggatgcatc ctgcgggaat atgtcttacg tgattttcaa cgatcaggtg attaaaccgc
    26401 gctttgaatg taagaccatc tatgaaatga ccagcgaact ggcaaaacgt cttggcgttg
    26461 agcaacagtt tactgaaggc cgtacacagg aagagtggat gcggcatctg tatgcccagt
    26521 cgcgggaagc gattcctgaa ctgccaacgt ttgaagagtt ccgcaagcag gggatcttta
    26581 aaaagcgcga cccacaaggg catcacgttg cttataaagc cttccgtgaa gatccgcagg
    26641 caaacccact gactacgcca tcgggcaaaa ttgagattta ttcgcaggcg ctggctgaca
    26701 ttgccgctac ctgggaattg ccagaaggcg atgtgatcga tccactgccg atctacacgc
    26761 cgggctttga aagttatcag gatccgctga acaaacagta tccgctgcag cttacgggtt
    26821 tccactataa atctcgcgtt cactcaactt acggcaacgt tgatgtgctg aaagcggctt
    26881 gccgtcagga aatgtggatc aacccgcttg atgcgcaaaa acgcggtatc aacaacggcg
    26941 ataaagtcag gatctttaac gatcgtggtg aagttcatat tgaggcgaaa gtgacgccac
    27001 gaatgatgcc gggtgtggtc gcactgggtg aaggtgcctg gtatgacccg gatgcaaaac
    27061 gtgtcgataa gggcggttgt attaacgtac tgaccactca acgtccgtct cctctcgcta
    27121 aggggaatcc gtcacataca aaccttgttc aggttgaaaa ggtgtaagga gtaaccgatg
    27181 acaacccagt atggattttt tattgattcc agccgttgca ccggttgcaa aacctgcgag
    27241 ctggcctgta aagactacaa agatttgacg ccagaagtca gcttccgccg catttatgaa
    27301 tatgctggcg gcgactggca ggaagataac ggtgtctggc accagaacgt gtttgcctac
    27361 tatctgtcga tttcatgtaa ccactgcgaa gatccggctt gtactaaagt ctgcccgagc
    27421 ggtgcgatgc ataaacgcga agatggtttt gttgtggtcg atgaagatgt atgcattggc
    27481 tgccgctact gtcatatggc ttgcccgtat ggcgcaccgc aatataacga aacgaaaggc
    27541 catatgacca aatgcgatgg ttgttatgac cgtgttgcgg agggtaaaaa gccgatctgt
    27601 gttgaatctt gtccgctgcg ggcgctggat ttcggcccta tcgacgagct gcgtaaaaaa
    27661 catggcgatc tggcggccgt tgcgccgttg ccgcgagctc actttaccaa accgaatatt
    27721 gtgatcaaac ccaatgccaa tagccgcccg accggggata ccactggcta tctggcaaac
    27781 ccgaaggagg tgtgagatgg gaagtggatg gcatgaatgg ccgctgatga tcttcacggt
    27841 cttcgggcaa tgtgtagcag gtggttttat cgttctggct ttggcgctgc tcaaaggcga
    27901 cctgcgagca gaagcccagc agcgtgttat cgcctgcatg tttggtttat gggtgctgat
    27961 gggcattggc tttatcgcct ctatgctcca tcttggttca ccaatgcgcg cttttaactc
    28021 gctcaaccgg gtaggggctt cagcactcag taacgaaatc gccagcggtt cgatcttttt
    28081 tgccgtaggc ggcatcggct ggttgctggc aatgctgaaa aagctgtcac cggcattgcg
    28141 tacgctgtgg ctgatagtga cgatggttct tggcgtcatc tttgtctgga tgatggtgcg
    28201 tgtgtataac agcattgata ccgttccgac ctggtacagc atctggacgc cgatgggctt
    28261 cttcctgacg atgtttatgg gcggcccgtt actgggttac ctgttgttga gtctggctgg
    28321 cgtcgatggc tgggcgatgc gtctgctgcc agccatttct gtactggcac tggtggtgag
    28381 tggcgtgatg tcagtgatgc agggcacaga gctggcaact attcatagtt ctgtgcagca
    28441 ggcagcagcg ctggtgccgg actatggtgc gctgatgtcc tggcggatcg tgcttttggc
    28501 cgttgccctg tgcttgtgga ttgcaccaca gctaaaaggt tatcagcctg cggttccgct
    28561 actatccgtc tcattcattc tgcttctggc aggggaatta atcggtcgcg gcgtattcta
    28621 tggtttgcat atgaccgtgg ggatggccgt cgcaagctaa tcagtaacaa ccggggtttc
    28681 ggccccggtt ctcttttatt tctgcttcgt taacgtgtca taactggtca tcaaattacg
    28741 ataatcagga atatggttag agaacaacgt cgccagtcct tcaatatcat tacgccagtc
    28801 acggtgcagc tcgcacgcca cgccgaacca tgtcatcaat tgcgcacctg cttgcgacat
    28861 gcgatcccat gccgaatgcc gggtaatttc attaaaggtc cctgacgcgt cagtcacaac
    28921 aaagacatca aacccttctt caatggctga aagcgccggg aatgccacgc aaacttcggt
    28981 taccacaccg gcaataatta actgtttttt acctgtcgct ttgactgctt ttacaaaatc
    29041 ttcgttatcc caggcgttaa tatttcccgg gcgagcaatg taaggtgcat cggggaattg
    29101 tgctttcagt tctggaacta atggaccgtt aggaccggtt tcaaaactgg tggtaagaat
    29161 agtaggtaag ttgaaatact tagccaggtc acccagcgcc agcacgttat ttttaaactt
    29221 atcgggttcg atatcccgta caagggaaag taaaccagcc tgatgatcaa caagcaaaac
    29281 ggcagcatca tttttatcaa gacgaacata cggtttggtc atcctcttct cctttcgaga
    29341 gtcagtggta cgtctgaaat cggtcagacg tgatgcattg ctctgaaagc atagacggga
    29401 aatatgagtt tgctgtgacc atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt
    29461 tagatcaata aaaacaatca gatgagatta atcatctctt cagcattagc gttatgaatg
    29521 agtaatataa atcgataata ccgccgctgc gatatgcgcg tattttcacc cattgacaat
    29581 gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt
    29641 ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga
    29701 tcgcggtgtt aaatacactc gtgccgcttt ggctcgccca ggaacacatg tccacatggc
    29761 aggtaggcgt tgtcagctca tcctatttta ccggcaacct tgtcggtaca ttgctgacag
    29821 ggtatgtcat taagcgcatt ggctttaacc gcagctatta tctggcctcc ttcatttttg
    29881 ccgctggctg tgccggcctt ggcctgatga ttggattctg gagctggttg gcttggcgtt
    29941 ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt tgagagcgcg ctgatgtgca
    30001 gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg
    30061 gaacgttttt aggccagtta ctggtcagca aagtttcaac cgagctgatg tccgtattgc
    30121 cgtgggttac aggtttgacg ttggcaggga tcttaccgct gttgtttacg cgtgtgctga
    30181 atcagcaggc tgaaaaccat gattcgacgt caattacgtc aatgctaaaa ctccgtcagg
    30241 cgcggcttgg cgtgaatggc tgcattatct caggaatcgt tctgggatct ctatatggcc
    30301 tgatgccgct gtacctcaat cacaaagggg tgagcaatgc cagcattggt ttctggatgg
    30361 cggtactggt cagtgcgggt atccttggac aatggccgat tggacgtctg gcggataagt
    30421 ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt cattctcggc agtatcgcga
    30481 tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc
    30541 tatatccggt ggcgatggca tgggcttgcg agaaagttga acatcatcaa ctggtggcga
    30601 tgaaccaggc cttactgttg agctatactg tgggaagtct gcttggcccg tcatttaccg
    30661 ctatgctaat gcagaatttc tccgataatt tattgtttat catgatcgcc agcgtatcgt
    30721 ttatctattt gctgatgctg ctgcgcaacg ccggtcatac gccgaaaccc gttgctcacg
    30781 tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac actctgcttt tttgtttctt
    30841 ctatctgact tgctttattc caaattttat tcgtttaaaa ataaaatgtg cagcaggtta
    30901 taattttgca tttcgctatt tccgcacttc ttatttgccg cgcataatcc ctcgttttac
    30961 agatgcccct ttaattttgg cgaaggattt gtctatggct gggaatgttc aggaaaaaca
    31021 gttgcgatgg tacaacattg cgctgatgtc ttttatcact gtctggggtt ttggcaacgt
    31081 tgttaataac tatgccaacc aggggctggt ggttgttttt tcatgggtgt ttatctttgc
    31141 actctatttc acaccttatg cgctaattgt tggtcagtta ggctcgacct tcaaagatgg
    31201 gaagggcggg gtcagtacct ggattaaaca cacgatggga cccggactgg cttatctcgc
    31261 cgcgtggacc tactgggtgg tgcatattcc ctatctggca caaaaacccc aggcaattct
    31321 gattgcgctc ggttgggcga tgaaaggcga cggttcgcta atcaaagaat attcagtcgt
    31381 agcgttacag gggttaacgc tggtgctgtt tatcttcttt atgtgggttg cttcacgcgg
    31441 tatgaaatcg ctgaaaatcg tcggttctgt ggcagggatt gcgatgtttg ttatgtcgct
    31501 cctgtatgtg gcgatggcgg taaccgcgcc tgcgattact gaagtgcata ttgcgaccac
    31561 aaacattacc tgggaaacgt tcattcctca tatcgacttt acctacatta ccactatttc
    31621 aatgctggtt ttcgcggttg gcggagcaga gaagatttct ccttacgtta atcaaacgcg
    31681 caacccagga aaagaatttc caaaagggat gctatgcctg gcggtgatgg ttgcggtttg
    31741 tgccattctg ggctcactgg cgatggggat gatgtttgac tcgcgtaata tcccggatga
    31801 cttaatgact aacggtcaat attacgcctt tcagaagctg ggcgagtatt acaacatggg
    31861 taatacttta atggtgattt acgccattgc gaataccctg ggacaagtag ctgcgctggt
    31921 attctcgatt gatgccccgc ttaaagtgct attaggtgat gctgacagca aatatattcc
    31981 agccagttta tgtcgtacca acgcttctgg tacgcccgtt aatggctatt ttctgaccct
    32041 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
    32101 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
    32161 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
    32221 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctttgc
    32281 ctgcttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
    32341 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
    32401 ggcgcgtaaa gcgaatagta aataattatt gtggtcggtg acgctgactc tggcagatct
    32461 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
    32521 gataatagat ctggaagcct gaacgctctg tgctccagtc ttccagtaca cattctaaac
    32581 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
    32641 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
    32701 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
    32761 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
    32821 ctggcgttgc tgctacacat aatttcactg gcgggccgat cgccacgctg atcatatctt
    32881 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgacaa
    32941 gagagtcgtc agttgtgagc tcgactttaa tatcaggata ttcacgtgtg aaaccaacca
    33001 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
    33061 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
    33121 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
    33181 gattgaataa gcgaatttta agacgttgtt ccagtgtttt aatactatgg ctaatggcgg
    33241 acgaggataa gcctaattca tcgcccgctg cacgaaagct ttgattacgg gccacggcaa
    33301 agaaagtggc aaagtcagac atattcatcc gcattagtga aaatccttca ttaactcatc
    33361 ctgaatcatc acgtttacag gattatgccg cctgcgtaaa gtgcgctcca gaacttaacg
    33421 tggaggtaaa attatgcagt ctgaacgtat ttatttggta tgggcccatc ctcgtcatga
    33481 ttcattgacc gcacatattg ctgatgcgat ccatcagcgg gcaatggagc ggaaaataca
    33541 ggtgacggaa ctcgatttat atcggcgtaa tttcaacccg gtgatgacgt cggaagatga
    33601 accagactgg aagaatatgg ataaacgtta ttctccagag gttcatcagc tttattcaga
    33661 gctgcttgaa catgacacgt tagtggtggt ttttcctctc tggtggtaca gcttcccggc
    33721 aatgctaaaa ggatatattg acagagtatg gaataagggg ctggcttatg gagatgggca
    33781 caaattacca ttcaataaag ttcgttgggt ggcgctggtt ggaggagaca aagaatcatt
    33841 tgtccagatg ggctgggaaa aaaatataag cgattatttg aaaaatatgt gcagttatct
    33901 tggtattgaa gatgccgatg tcactttctt gtgtaataca gtggtattcg atgggcaaga
    33961 acttcatgcg agctattatc agtcgttatt atctcaggta cgggatatgg tagatgcact
    34021 ataaaatgtg ttaaaaacgc tgtagcagaa tgaagcgcgg aataaaaaag cggcaactca
    34081 ataaagttgc cgctttacgg ggaaattaga acattacctt atgaccgtac tgctcaagaa
    34141 tgcctttcac gcgttccatg gtctctttct taggtggttt aacaccgtcg agtttgtact
    34201 cttcacccat tgccacccat ttgtgtttgc ccagctcgtg gtaggggaga agctcgattt
    34261 tctcaacgtt gcccatatca cgggtaaatt caccgaggcg atgcgctgaa tcgtcatcgt
    34321 cagaccagcc tgggacaaca acgtagcgga tccacacctt cacatttttg ttcgccagat
    34381 atttggcgaa ctccagcgtg cggtgattgg aaactccaac cagattttgg tggatctcgt
    34441 cgttcatctg tttgagatcg agcattacca ggtcggttac ttccagcagt tcatcaatca
    34501 ccggatcgta acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg ccttcttttt
    34561 tgcaggcgcg gaaccagtca cgaacaaact cagcttgtag gattgcctca ccgccggatg
    34621 cggtaacgcc gccgccggaa gcgttcataa agtggcgata ggtcaccacc tctttcatta
    34681 aatcttcaac ggtaacttct ttaccgccat gcgtatccca ggtgtcgcgg ttatgacaat
    34741 acaggcagcg catcaggcag ccctggaaaa aggtgataaa gcgaatacct gggccgtcta
    34801 cggttccaca ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc
    34861 agatgtggcc catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt
    34921 tggcagtcac cttagagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc
    34981 cactttcgtg gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta
    35041 catagattga gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt
    35101 gaaacgtact gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc
    35161 catcgcgtcg agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc
    35221 gatggatgct tcgtggtgga agtaaccatc catcagacca gccaggttgg tcttacgaac
    35281 ttcgtcgtct ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt
    35341 agcgtaagca aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc
    35401 acgaccgtgc atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg
    35461 ggtgttacca gttttcttac catacacaac gttagaagtg atggtcagaa cagactgagt
    35521 cgggatagcg tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag
    35581 gtcaacagcc aggtcatcta cacgcggatc attgttacca aactgcgggt attcgccttc
    35641 gatttcgaag tcgatagcaa gaccgtcttc gtcacgaatc ggtttaactt tcgcatattt
    35701 gattgcagac agggagtcag cagcaacgga cagaccagcg ataccacacg ccatggtgcg
    35761 gataacgtca cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat
    35821 gtagtggatg atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc
    35881 catgcgctcc atcacttcat cgtagttcag gagatcgcct ttgatcggtt cagacttcgg
    35941 accaacctgc attttcagtt tttcgtcaac gccgccgttg attgcgtaca gcatggtttt
    36001 cgccaggttt gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac
    36061 gcagcaagcg atagcatagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc
    36121 atactgcaga gaagaggtgt cgatggacac tttagcggcg aatttcttga agttcagcgg
    36181 cagtttttca gaccacagaa tggtcatgtt cggttccgga gacggaccca tggtgtacag
    36241 ggtgttcagg aaacggaagc tgtttttggt aaccagggta cgaccgtcga ggcccatacc
    36301 accaatagat tcggttgccc agatcgggtc gccagagaac agttcatcgt attccggagt
    36361 acgcaggaag cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc
    36421 ttgttcggtg atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga
    36481 ggtacgaccg aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa
    36541 gtaagtccac tggatagctt cctgagcgtt ggtagccgga ccagagatgt cgtagccgta
    36601 tttcgcagcc atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg
    36661 cagacggata gtctgttcca ggtttacgcc gttttccaga tcagtctgca gagaggtgaa
    36721 ctgagcgtat ttgtctttca tcaggtagtc gataccgtac agcgcaacgc gacggtagtc
    36781 accgatgata cggccacggc cataagcatc tggcagaccg gtcagaacac cggatttacg
    36841 gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta
    36901 ttcagtgaag atttttttga tcatcgggtc cagttcgcgg ttgtacgctt tgcaggaacc
    36961 ttcgatcatt ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg
    37021 cagaccaaca actttttcca acgctttgtt gatgtaacca gcgtcgtgag aggtgatggt
    37081 agaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca gtttaacgcc
    37141 ttccattact ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc
    37201 gtcaccctcg tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg
    37261 ccagtcacct ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat
    37321 gtaacaccta ccttcttaag tggatttttt atttactgcg tacttcgaca accattaatg
    37381 gtgatcgttt tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccgcc
    37441 gataatatta ccgatcgtaa ccggaatcag gttatcagtg atgaagttca tcacggtcag
    37501 gtgagaaaaa ttttccggtg cagaaccgac agcggtccag aattccggag atgcgaagtc
    37561 gcggattaca atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct
    37621 ggcaacaaac atcgcgaccg gcagcaccat aatgaacgct ttgtccatca ggctacggcc
    37681 agaataactc atccatactg ccagacatac catcaggttt gccaggatac caaggcagac
    37741 ggcctcaata aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca
    37801 ttgaccattt gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc
    37861 gccgaccagg ttgccaaaat agacatttag ccagtttttc gccaactgac cccaggtgat
    37921 gcgcccactc gccttagcaa caacaatcaa cactgtggaa gtaaagagat cggctccgca
    37981 gacaacacaa agaatcagcc ccagagagaa gcaaatgccg ccaaccagtt ttgccatgcc
    38041 gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat
    38101 gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata
    38161 gacacccgcc tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt
    38221 gtcagctttc acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt
    38281 ata
//
LOCUS       DAMZII010000034        38018 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZII010000034 DAMZII010000000
VERSION     DAMZII010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.034"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            124..1488
                     /locus_tag="POA77_001675"
     CDS             124..1488
                     /locus_tag="POA77_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365124.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="POA77_001676"
     CDS             complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="POA77_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDI6365125.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2275..3186
                     /gene="panE"
                     /locus_tag="POA77_001677"
     CDS             2275..3186
                     /gene="panE"
                     /locus_tag="POA77_001677"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI6365126.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3149..3739
                     /gene="yajL"
                     /locus_tag="POA77_001678"
     CDS             3149..3739
                     /gene="yajL"
                     /locus_tag="POA77_001678"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDI6365127.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="POA77_001679"
     CDS             complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="POA77_001679"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI6365128.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5447..5689
                     /gene="xseB"
                     /locus_tag="POA77_001680"
     CDS             5447..5689
                     /gene="xseB"
                     /locus_tag="POA77_001680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI6365129.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5689..6588
                     /gene="ispA"
                     /locus_tag="POA77_001681"
     CDS             5689..6588
                     /gene="ispA"
                     /locus_tag="POA77_001681"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDI6365130.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6613..8475
                     /gene="dxs"
                     /locus_tag="POA77_001682"
     CDS             6613..8475
                     /gene="dxs"
                     /locus_tag="POA77_001682"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDI6365131.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLGAAGMEAKIKAWLA"
     gene            8530..9504
                     /gene="yajO"
                     /locus_tag="POA77_001683"
     CDS             8530..9504
                     /gene="yajO"
                     /locus_tag="POA77_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDI6365132.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="POA77_001684"
     CDS             complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="POA77_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDI6365133.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="POA77_001685"
     CDS             complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="POA77_001685"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDI6365134.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="POA77_001686"
     CDS             complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="POA77_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI6365135.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="POA77_001687"
     CDS             complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="POA77_001687"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI6365136.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="POA77_001688"
     CDS             complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="POA77_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI6365137.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVILMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="POA77_001689"
     CDS             complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="POA77_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDI6365138.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13814..14353
                     /locus_tag="POA77_001690"
     CDS             13814..14353
                     /locus_tag="POA77_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI6365139.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14652..15536
                     /gene="tsx"
                     /locus_tag="POA77_001691"
     CDS             14652..15536
                     /gene="tsx"
                     /locus_tag="POA77_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDI6365140.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15713..16060)
                     /locus_tag="POA77_001692"
     CDS             complement(15713..16060)
                     /locus_tag="POA77_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDI6365141.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16189..17160)
                     /gene="secF"
                     /locus_tag="POA77_001693"
     CDS             complement(16189..17160)
                     /gene="secF"
                     /locus_tag="POA77_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDI6365142.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17171..19018)
                     /gene="secD"
                     /locus_tag="POA77_001694"
     CDS             complement(17171..19018)
                     /gene="secD"
                     /locus_tag="POA77_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDI6365143.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19046..19378)
                     /gene="yajC"
                     /locus_tag="POA77_001695"
     CDS             complement(19046..19378)
                     /gene="yajC"
                     /locus_tag="POA77_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI6365144.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19401..20528)
                     /gene="tgt"
                     /locus_tag="POA77_001696"
     CDS             complement(19401..20528)
                     /gene="tgt"
                     /locus_tag="POA77_001696"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI6365145.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20584..21654)
                     /gene="queA"
                     /locus_tag="POA77_001697"
     CDS             complement(20584..21654)
                     /gene="queA"
                     /locus_tag="POA77_001697"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI6365146.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21747..22328
                     /gene="acpH"
                     /locus_tag="POA77_001698"
     CDS             21747..22328
                     /gene="acpH"
                     /locus_tag="POA77_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDI6365147.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFVNLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22333..24150)
                     /gene="malZ"
                     /locus_tag="POA77_001699"
     CDS             complement(22333..24150)
                     /gene="malZ"
                     /locus_tag="POA77_001699"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDI6365148.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(24306..25679)
                     /gene="proY"
                     /locus_tag="POA77_001700"
     CDS             complement(24306..25679)
                     /gene="proY"
                     /locus_tag="POA77_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDI6365149.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25755..27074)
                     /gene="brnQ"
                     /locus_tag="POA77_001701"
     CDS             complement(25755..27074)
                     /gene="brnQ"
                     /locus_tag="POA77_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDI6365150.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSVYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27481..28776)
                     /gene="phoR"
                     /locus_tag="POA77_001702"
     CDS             complement(27481..28776)
                     /gene="phoR"
                     /locus_tag="POA77_001702"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDI6365151.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28834..29523)
                     /gene="phoB"
                     /locus_tag="POA77_001703"
     CDS             complement(28834..29523)
                     /gene="phoB"
                     /locus_tag="POA77_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDI6365152.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29713..30915
                     /gene="sbcD"
                     /locus_tag="POA77_001704"
     CDS             29713..30915
                     /gene="sbcD"
                     /locus_tag="POA77_001704"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDI6365153.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30912..34055
                     /gene="sbcC"
                     /locus_tag="POA77_001705"
     CDS             30912..34055
                     /gene="sbcC"
                     /locus_tag="POA77_001705"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDI6365154.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQTSGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34181..35365
                     /gene="araJ"
                     /locus_tag="POA77_001706"
     CDS             34181..35365
                     /gene="araJ"
                     /locus_tag="POA77_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDI6365155.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35508..36416)
                     /gene="mak"
                     /locus_tag="POA77_001707"
     CDS             complement(35508..36416)
                     /gene="mak"
                     /locus_tag="POA77_001707"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDI6365156.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36541..37452
                     /gene="rdgC"
                     /locus_tag="POA77_001708"
     CDS             36541..37452
                     /gene="rdgC"
                     /locus_tag="POA77_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDI6365157.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37530..37949)
                     /locus_tag="POA77_001709"
     CDS             complement(37530..37949)
                     /locus_tag="POA77_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365158.1"
                     /translation="MYDLLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLQHKTHNIAALKIIARRSE"
BASE COUNT         9391 a        10232 c         9676 g         8719 t
ORIGIN      
        1 gaatgcaaaa aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa
       61 cactctgttt acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg
      121 gtaatgaacg attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc
      181 gtattctcgt tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc
      241 atggctctgc aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg
      301 actcaggccg tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca
      361 ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac
      421 tccatctggg gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc
      481 gttatggcgc tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt
      541 atcggcgtga gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact
      601 cacaaacttg ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt
      661 gcgttgacca tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga
      721 atggtgaaag gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt
      781 ggcattatgt gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta
      841 gctgatgcgg ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc
      901 gcctttggct cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa
      961 gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa
     1021 acgcagttct ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg
     1081 gaagcccttc tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg
     1141 atgggtgtct actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc
     1201 tggattgacg gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca
     1261 gtgtggctgg cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt
     1321 gaaatcccgg cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa
     1381 ggcatcaaag aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc
     1441 aaagtgacga atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga
     1501 attgcctgct gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag
     1561 gccgaatcag gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat
     1621 cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga
     1681 cagcctgcaa atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg
     1741 cctgcacttt cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct
     1801 caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct
     1861 ccggtacatc cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat
     1921 ccagcaactg attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt
     1981 cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc
     2041 tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag
     2101 atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat
     2161 aaccgacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac
     2221 aattaaggga tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa
     2281 attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag
     2341 ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt
     2401 gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc
     2461 accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc
     2521 ctcgcgtcca cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc
     2581 atcgaagagt tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc
     2641 cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca
     2701 cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac
     2761 gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg
     2821 attaatccac tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa
     2881 gaaattatgc agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact
     2941 tcagcagaag atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc
     3001 tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt
     3061 tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt
     3121 gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc
     3181 tggtagtgaa gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa
     3241 agtcaccact gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt
     3301 gaagctgctg gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt
     3361 actgcctggt ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac
     3421 cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac
     3481 cgtgctggtg ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa
     3541 agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa
     3601 attgctgacc agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct
     3661 gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat
     3721 ttataattat tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt
     3781 tacaaccaca aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata
     3841 gagcgcctgc agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg
     3901 gtcgagatcg ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc
     3961 ttcgactttc agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc
     4021 gttcgggccg aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc
     4081 gatttcgcgg atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc
     4141 gaatttctct tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga
     4201 gataacaccg caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc
     4261 caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga
     4321 gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc
     4381 gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat
     4441 catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac
     4501 gaccggttcg aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca
     4561 cagataatgc gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa
     4621 acagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa
     4681 accaccggaa atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc
     4741 ttcgtagcgg cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac
     4801 ttccggattg gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac
     4861 gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca
     4921 gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg
     4981 catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt
     5041 cagggcgtcg cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc
     5101 ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc
     5161 ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa
     5221 caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta
     5281 tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta
     5341 acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt
     5401 ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa
     5461 tgaggctccc gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct
     5521 ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct
     5581 ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc
     5641 tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg
     5701 cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca
     5761 ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt
     5821 aagcgtctgc gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac
     5881 acgctggacg cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat
     5941 gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag
     6001 tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt
     6061 ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa
     6121 ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg
     6181 gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca
     6241 ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct
     6301 ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac
     6361 atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa
     6421 cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg
     6481 gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc
     6541 tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt
     6601 aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     6661 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     6721 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     6781 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     6841 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     6901 accatccgtc agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac
     6961 gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct
     7021 gccgagaaag aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc
     7081 gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     7141 gtcctcaacg acaacgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg
     7201 gcgcagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc
     7261 tctggcgttc cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg
     7321 gtagtgcctg gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt
     7381 cacgatgtgc tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag
     7441 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc
     7501 actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc
     7561 ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac
     7621 aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca
     7681 cgtaaattcc cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt
     7741 gccgccggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg
     7801 caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc
     7861 gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac
     7921 ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa
     7981 tgtcgccaga tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac
     8041 ccgcgcggta acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa
     8101 ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca
     8161 gacgcggcga aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa
     8221 ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta
     8281 gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat
     8341 cgtaaaccag tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact
     8401 caggaagaaa tgcgcgccga actcggcctc ggtgccgccg gtatggaagc caaaatcaag
     8461 gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg
     8521 agtggcagca tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc
     8581 ctcggctgta tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa
     8641 gaaagcagcc gtcccataat taaacgtgca ctggaaggcg gcataaattt ctttgatacc
     8701 gccaatagct attccgatgg cagcagcgaa gagatcgttg gtcgcgcact gcgggatttc
     8761 gcccgtcgtg aagacgtggt cgttgcgacg aaagtgttcc atcgcgttgg tgatttaccg
     8821 gaaggattat cccgtgcaca aattttgcgc tctatcgacg acagcctgcg acgtctcggc
     8881 atggattatg tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag
     8941 acgctggaag ctctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca
     9001 tcaatgcacg cttcgcagtt tgctcaggca ctggaactcc aaaaacagca cggctgggcg
     9061 cagtttgtca gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg
     9121 ctgccactgt gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg
     9181 cgactgacgc gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa
     9241 aatctctata aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacaggcgtc
     9301 agcgaagaac tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg
     9361 ggcattgccg caccgattat cggaacttcg cgcgaagaac agcttgatga gctattgaac
     9421 gcggtggata tcactttgaa gccggaacag attgccgaac tggaaacgcc gtataaaccg
     9481 catccggtcg taggatttaa ataagagtcc aggcctgatg agacgcaaca agcgtcacat
     9541 caggcactgg tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac
     9601 aggatacctg cggagatcac cccggcgaca atatcatcga tcatgatccc catgccgcca
     9661 tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa
     9721 atgacgaatc cggcagtgac ccactgccag tcattggtcg gcaatgccat gagcgtgatc
     9781 cacatgccga taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc
     9841 gccgtttgat gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag
     9901 agctgccagg gtaaaaaggt catcagatac cagaaaggga tcgccgccag cgatcccatc
     9961 gtcccaggaa cgatcgggct taatccactg ccgaatccga cagcaagcag atgccacgga
    10021 ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc
    10081 tttccagtca aaggtgacag gttcgccgtc acggataaaa caaagccctt cgatatcggc
    10141 ggtcatctgc ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc
    10201 gccacggttc agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc
    10261 ccagcgcagc gcctgttcag gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa
    10321 tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat
    10381 cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa
    10441 aatacgcggc gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc
    10501 aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt
    10561 cacatagatc cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac
    10621 aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag
    10681 ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc
    10741 ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc
    10801 tgccagatcg cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc
    10861 atggaggaag tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc
    10921 ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact
    10981 tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc
    11041 ctcagcaggt aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg
    11101 cctggatatc actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg
    11161 acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg
    11221 gccactttgt atggcacatc gctacgttta gacagctcgt acagcgcaat gcgcagtact
    11281 gctttttcta cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg
    11341 tcgaggtatg cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca
    11401 acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg
    11461 gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca
    11521 ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat
    11581 ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc
    11641 gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc
    11701 ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt
    11761 gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag
    11821 tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc
    11881 atcttttacc tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt
    11941 gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt
    12001 agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga
    12061 ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta
    12121 aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt
    12181 cggctaattt ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt
    12241 taggtgcgat atagacaatc agctcatcga ctaaacccgc ctgcagcaat gcgccagcga
    12301 gcgttggccc cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca
    12361 gtataaccag atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg
    12421 gccactcact agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa
    12481 tgcgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga
    12541 gattttgttg cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta
    12601 aggctggatc atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc
    12661 gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg
    12721 ccatcgccgt gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg
    12781 tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt
    12841 ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt
    12901 taggatcttg catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc
    12961 acggtggcgt acgaccatga tggctacagg gttcgagtgt gacataggcg gtcgcacctt
    13021 tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgcacgtt
    13081 ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt
    13141 tgggatgcgt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact
    13201 cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt
    13261 cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt
    13321 gctccatcac cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca
    13381 gctgcgattt aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt
    13441 ccagcgcccg cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt
    13501 tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat
    13561 tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt
    13621 caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg
    13681 tggttaacag aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg
    13741 agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag
    13801 aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag
    13861 ccttagcgcc tgtgtgcagc agagcgaagt gcgacagatg aaacacagcg tcagcacgct
    13921 gaaccaggag atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct
    13981 gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact
    14041 ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg
    14101 caccaccctg acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg
    14161 aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca
    14221 gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt
    14281 acaattaaag gggatttctg tggatcagtt aggctttgta cgtatccacg acattcaacc
    14341 tgttatgcag taaacgtatt tcgggacgat tttgtgcgtc ccgcaacata tttccccgtc
    14401 attttgttac tctgcttaca tcacctggat tgatagtaaa agtttgcaac aagggcgaaa
    14461 gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat
    14521 agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta
    14581 tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca
    14641 gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt
    14701 cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc
    14761 agagcgttaa cgttgtcgga agctatcaca cccgttttgg accgcagatc cgcaacgata
    14821 cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg
    14881 atgcgccggt attcttcggc ggtaactccg atgccaaagg tatctggaac cacggctctc
    14941 cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta
    15001 gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta
    15061 ataaagatgg tcgccagagc acctggtata tgggtctggg taccgacatc gatactggtc
    15121 tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag
    15181 cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg
    15241 atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt
    15301 taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat aactctatcg
    15361 cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt
    15421 actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact
    15481 tcaacgttcg ctctaccggc tggggtggtt acctggttgt aggttacaac ttctgattat
    15541 gaaaatgccg ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg
    15601 ccggatgcgg cgtaaacgct ttgtccgacc cacaaaagca cgtaaattca atatattgct
    15661 gaaataacgt agtcctgata agcgtagcgc atcaggcaat tttacgtttt aatcacttct
    15721 tcttgttcat catcgctttc aggtcagcga atgggttgta cttcgcttca ccgacatctt
    15781 tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg gtatatttcg
    15841 aatgctcatg atcgtggcaa tagagacaaa gcaattccca gttactgcca tcttccgggt
    15901 tattggtatg gtcgtgatca atgtggtgaa cagtaagttc acgcaggttg gaatagacaa
    15961 actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt tcgcgatagc
    16021 cgctttctaa ccgcgcatag ttttttggga tgatagccat gaaaagttac ctggatgaaa
    16081 tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatctcca tacatcaaaa
    16141 aaaatcccga tcttctgacc gggattttca acatcaacgg gaacttgatt aaggcagaat
    16201 tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt cgcgcttcat
    16261 acccagtttc agagccaacg cagatgccac atagatggaa gatgcagtac cgatggaaac
    16321 accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga ggtacagcat
    16381 caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg tctgggtcaa
    16441 ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt tttcacgaat
    16501 acggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca tcaacgatgc
    16561 cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac
    16621 gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc cactcaaagc ggaaacctac
    16681 gtacacgagg atagacagca gcgctgccat caacgccatc gcaccggttt gcgcaaggtc
    16741 tgcccccacg ctcggaccga cgaactcaat acgcttcact gctgcattct gattggtgga
    16801 ttcgttaatc accttcagaa cctggctgcc cagcacctga ccgccggttt cgccttcagc
    16861 aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca ttggctcttc
    16921 aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt
    16981 aatttcgata accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat
    17041 aataacgata gcggcgatta acagcagacc agagatgccg aaagcccagt agtcccagcg
    17101 cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac
    17161 atcgcactcc tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga
    17221 tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa
    17281 tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg
    17341 tggtgatgtt cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct
    17401 gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc agtacgttcg
    17461 catcgaccgc caccgcaagg gttaagacga tacccgcgat acctggcata ctcagcgttg
    17521 cgcctggcaa cagcgacata atgccgacga ttaagatcaa gttggcaatc agagcactgg
    17581 tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac
    17641 cggcaaggca agcctccagc ccctgttcaa tgttctgcat acccagggtt gggccaatgg
    17701 tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca
    17761 gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta cccagacgag
    17821 actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccatttg
    17881 catctttctt accgctgtct ttgtactcca caaacagggt tgccatcggt ttgccgatgt
    17941 tgtccttagt gaagttagac atgatgttac caccagcgct atcgagcgag atgttaacct
    18001 gcggttggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca ccggtcagaa
    18061 ttacgcgttt gtacagcaca actggctgac cttcgcgggt ctgtttcact tcagagtcgc
    18121 ccggtacgcg accggatgcc gcagcggcct ggtcaacgtt ggtgtttacc agacggaatt
    18181 ccagcgttgc cgtcgcaccc agaatctctt tcgcacgcgc agtgtcctga atacctggca
    18241 gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc acgccaagtt
    18301 ggtttacacg gttacgcagg atattgatgt tctgctgcac cgcatattca cgcgcttcac
    18361 tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggcta ctaatcacca
    18421 ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga
    18481 aagtaatgct caggccgtag ttgttttctt tacgaacggt ggtatacggg atgccctttt
    18541 cacgcagatc gctgcgcagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt
    18601 ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg
    18661 gctcagcgtg aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga
    18721 cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcagctgg gtgtcagtgg
    18781 agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt
    18841 cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg
    18901 caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac
    18961 cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg
    19021 caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg
    19081 cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt
    19141 agccgttttc cgctactttg gttacgcgac caaccaggcc accgttcgtc agaacttcat
    19201 cacctttggc aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac
    19261 gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg
    19321 ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta
    19381 aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc
    19441 tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc
    19501 gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc gcctaatatt
    19561 tcgttgcaac ggtcaagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa
    19621 tcacactcag gatcgagtgg gccagtatcg ctcttatact tcgcattgcg gattttcacc
    19681 acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca
    19741 aacatatcga taccacgacg tacgccttca accaggtctt ctggtttacc aacgcccatc
    19801 aggtaacgcg gtttgtctgc cggaatttgc gggcatacat gctccagaat gcggtgcata
    19861 tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa accgatatct
    19921 accagacctt taacagaaat atcacgtaaa tcttcgtaaa cgctgccctg aatgatacca
    19981 aacagcgcat ttttgtttcc gagactgtca aaacgctcac ggctacgctt cgcccaacgc
    20041 agagacatct ccatggagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca
    20101 tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccattga tttttcagga
    20161 tcgaggaaaa tcggatcgcc gttgatcggg ttacggaagt gcacgccctg ttcggtgatt
    20221 ttacgaatat cgccaaggct gaagacctgg aaaccgccgg agtcggtgag gatcggcccc
    20281 ttccactgca taaaatcgtg cagatcgccg tgcagtttca tgatttcctg gcccgggcgc
    20341 agccacaggt ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttcc
    20401 ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact
    20461 acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagttca
    20521 aatttcattt tttctccact acgtcagaaa aacagtccaa cgttttaaac cagcgccgcg
    20581 gaattactcc ccgacgcgct cattaattgc ctgcggattg tacgtgataa acatcgcatc
    20641 accgtaacta aaaaagcgat atttctcttc taccgctgct ttataggcat tcatggtgtg
    20701 ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc gattccggca ggtggaagtt
    20761 tgtcaccagc gcatcgacca ctttgtactg gaagcccgga tagataaaga tttgggtatc
    20821 gtcaaagaac ggttcgataa gatcgttttt cgccgcctga gccgcgcttt ccagcgaacg
    20881 tactgaggtg gtgccaaccg caatcacccg gttaccgcgc gctttcgccg ccagtaccgc
    20941 gtctaccaca tcctgcggca cttcagcgta ttccgagtgc atgatgtgat cttcaatggt
    21001 gtcgacgcgc accggctgga aggtgcccgc accaacgtgc aacgtcacaa acgccatctc
    21061 cacgcctttg gcgcgtaatt tttccagcaa aggttcgtca aaatgcagac ctgcggtcgg
    21121 ggctgcaacc gcgcccggtt tttcgctata aacggtttga taaagttcgc ggtcagcgtc
    21181 ttcgtccgga cggtcgatat acggcggcag cggcatatgg ccgatgctgt tgagaatatc
    21241 cagcaccgag cgttcatcat taaattcgac ttcaaacagt gcgccgtggc gcgcggtcat
    21301 tgttgcgtta atactttcgt catcgcccag cagcagttct gcgccaggtt ttggcgcttt
    21361 cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc atccgttcaa ccagcacttc
    21421 aatcttgccg ccgctggctt tacgcccaaa caggcgcgcc gggatcacgc gggtattatt
    21481 aaaaaccaga agatcgccgg ggttgagctt atcaagtaaa tcggtgaaag taccgtgcgt
    21541 cagcgcgccc gtcggcccgt ccagcgacag taaacgacag ctactgcgtt caggcatggg
    21601 atagtgggca atcagggatt cgggcaattc aaaggagaaa tcggtaacgc gcatgacact
    21661 gactcagact aaaataagag gcgggtagtc tagtgccggg gcgctctccc tgcaaccttt
    21721 accccctttt ggatagaata taatcgatga attttttagc tcacctgcat ttagcccatc
    21781 tcgcggaaag ctcgctttcc ggcaatttac tggctgattt cgtacgcgga aatcctgaag
    21841 aaagttttcc gcccgacgtc gtggctggca ttcatatgca tcgacgtatc gacgtattaa
    21901 ccgacaatct gccggaagtc cgcgaagcac gggaatggtt tcgtagtgaa actcgccgcg
    21961 ttgcgcctat tacgctggat gtcatgtggg atcactttct ttcccgccac tggtcgcagc
    22021 tgtcgccgga ctttccgcta caggaatttg tctgttatgc ccgcgagcaa gtgatgacga
    22081 ttttgccgga ctcaccgcca cgttttgtca atctgaacaa ttacttgtgg tcagagcagt
    22141 ggctggtgcg ctatcgcgat atggatttca tccagaacgt gttaaacggc atggcaagcc
    22201 gccgcccacg tctggatgcc ctgcgtgact cctggtacga tttagacgct cattatgacg
    22261 ccctcgaaac ccgcttctgg cagttttatc cgcggatgat ggcgcaggcg tcacacaagg
    22321 cgttataaaa cgttagttca tccataccgt agccgaaatg gcaggcaaag ccagaatccc
    22381 gtttgtcagt tgcccatggc cttctttgcg ttgccattgc gcgacattaa gaaacggtga
    22441 cgcggggagc accacttcac aggcctcgcc acggttgatt gccaccagta cacgttgctg
    22501 attcagcacg cggacaaata ccactacgtt atcttccgca tacagcacct gacagccgcc
    22561 acgacgcagc gcctgacttt tcttacgcag cgcaatcatt cgctggtaca gcgcgaataa
    22621 cgccgtatcc tgcttttcca cctgccaggg gaacggttta cggcaaaacg gatcgttttt
    22681 gccatccagt cctacttcat caccgtaata aatgcacggt acaccaggcc aggtgaacag
    22741 ccagaccacc gccagcggca ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc
    22801 agtatcgtgg ctgtcgagct gattaaacat acgtaattgt tgttgatgag aaagccctgc
    22861 gcggtaatta tccatccagg ccatacaggt ttgggcatca atttgctgcg gatcgtaaga
    22921 gatatcggta ttggcaagaa atccccacaa cgggaatgtg aagccacgat agttcatggc
    22981 ggcatcttcc acatcggcct gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat
    23041 atacgcttcc ggctgggttt ctttcgccgc ttcggtgatc ccagcaacgt gctgcatatt
    23101 attgcgcgcc ccacccgcct cccccagcat atgcaccaca tccagccgcc agccgtccat
    23161 actccacggc gctttcagcc aatggcggac aatactgtct tccccgcgat aaatttcatt
    23221 caccagactt tccgactgat aatccagctt cggcaagctg gcatagccaa gccagtcgag
    23281 cgccgtgcca tcatcactaa acgagtacca gtcgcgccag ggcgattcag ggttgtgaca
    23341 agctcccccc gtgccacgat tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt
    23401 aaacacgccg tccagcacca gccgcattcc cagctgctgc gtattgtgtc gcaaacgcag
    23461 caacgcccca tcaccgccaa actgcggatc gacatggcga taatcctcgg tatcgtattt
    23521 atgtacgctg ggagctttaa acaccggatt gagatacagc gccgtcacgc caagcttttt
    23581 cagatacggc agtttttcgc ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc
    23641 cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg atctcttgtc cggctgcatg
    23701 atggtaatag acatgatcct gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg
    23761 gaagatctga taaaaaatct gatccgcagc ccattgtggg ccgatatccg gtacatcgac
    23821 ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag ccctgcggtg taaaccaacg
    23881 ctggcgatcg tgccacagca gtttgaaact gtaacgccgc cggggttgtc cgctggagag
    23941 atcaatcgcc gcacgccatg cggtgacgcc aggctgctgc tgactgcgct gcttatgcat
    24001 cgatactgac atttcttcgt tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc
    24061 ttcgcccgtc agccacagtg taatgagcag ttgatctttg ctttgtttaa caaatggggg
    24121 caccggcagg tgccatgcat ttaacatcat aattcccctg tgatgaaaat ctgatcacct
    24181 tgccatagcc taatgcagga tcactcatca tctgaaggat atttggggga ggaggacggg
    24241 ggcggataac tttcggaata gccggatcag gatttcctcc tgaaccggct tacagaacgc
    24301 aagtattatt ggttttcagc cagctgacga tcgtggcggc gtttaaacat ccagccaatc
    24361 aacagcacaa caatccacgc gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa
    24421 ccaatcaacc cgataataaa gagcaggaaa atcagcccgc cgatggtcgt tgctacccca
    24481 cccggcactt taaatttcag cgccttaact tcttctggcg gcaaacggcg acggaaggca
    24541 atttgcgaca gcaggatcat aatccacacc cacaccgtgg cgaaggttgc cagcgaagcg
    24601 atcaccagga agacgttttc cggcatgatg tagttcagat acaccgcaaa cagcagcgcg
    24661 gtagtcatca ccagcaccgt aacccacgga ataccgcgac gcgacgtttt gctgaaaatt
    24721 ttcggcgcgc tgccctgctc tgccataccg tggagcatac ggcctacgcc aaatacgtca
    24781 ctgttaattg ccgacagcga agcagtcagc acaacaaagt taagaatgct ggcggcaaag
    24841 gtaatgccca tatgctggaa cgtcagcacg aacgggctac cggcagtgcc aacctgattc
    24901 cacgggtaga tagacataat gacgaacagc gtaccgacgt agaacaccag aatacgcatc
    24961 ggcacggagt taatcgcacg cggtatcgat ttctcaggat ctttcgcttc accggcggta
    25021 atcccgataa tttcgatccc accgtaagca aacatcacca tttgcaacga catcaccatc
    25081 ccaagccagc cgttactgaa gaagccgccg ttgctccaca ggttatgaat accggtcggt
    25141 tgcccgccgt tgccaatccc ccagatgatg atgccgaaac cggcgacaat catgatgatg
    25201 atggtggcga ctttaaagaa cgagaaccag aattccagct caccgaagac cttcacgctc
    25261 atcaggttta cggcgcaaat gatcagcacc acgctcagta cccaaatcca gtgcggcacc
    25321 gtcgggaacc agacacccat atagatacca aaagcggtca catcggcgat ggcgacaata
    25381 aggatttcaa agcaataggt ccagccggta atgtaacctg ccagcgggcc gaggttttcc
    25441 tgcgcataac gcgagaaaga gctggctgcc gggttatgta ccgacatttc ccccagcgca
    25501 cgcataatga tatacgccgc gataccaccg ataatatagg ccaacaacac gctcggaccg
    25561 gccattttga tggcgtctgc cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt
    25621 gccataaagc gtatgtggcg ggtacttagc ccacgcttta gcttgttctt actttccatc
    25681 aattcctaac ccatcaacac aataaaaaac cacggggcat cgcagccccg tggttaaaac
    25741 aaatgttcag tgatttagtg agcgctggag gtcacctgac gacctgccgc gcgatcccag
    25801 ataatggcca gaaccaccat caccactgtt ggcattaacc acgccagacc ttgttcggcc
    25861 agcggtaaac gctgcgccca ggacggtaag atatcgctga atgcagatgc tttgatcccg
    25921 tcgagaatac caaaaagcag gctgataaac atcggcggag caatcacgcg ggacgaatta
    25981 tgccaccatg agcgtgtaaa acttaataca accagtgcga tacacggcgg ataaatagcg
    26041 gtcagcaccg gtacggagat ctggatcagc tggcttaagc cgaggttaga aaccaccatc
    26101 gagaagccgc cgaggataaa caccagcgta cgataagaga gcggtacgta ctgggcaaag
    26161 aattctgcac aagcacaggt caggccaact gccgttacca ggcaggcgat gaagattaac
    26221 gccgccagca ggaagctacc gccgccgcca aaggtgtgct gaacgtaagc atgcagaata
    26281 gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt ctgaacccag acggaacagc
    26341 gccaggtaca gcagagtcag accaacaccc gccatcaggc cagcccagac ggtataacgg
    26401 gtcagcagac gcgcttcggt aacgccacga gaacgcgccg cgttaacaat aacgatacca
    26461 aacaccattg cgcccagcgt atccatggtc agatagccgt taacgaagcc gttagaaaac
    26521 gcagcgtttt gataagcctc agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg
    26581 gcaacagaca ggatgaccag cgcgataatt ttcagcggcg caaggaagtt gcccacggta
    26641 tccagcagct tgcccggata gaccgaaacc agaataacga tagcgaaata gaccaggctg
    26701 tagataaaca gcggcagcgc ggaatcacca gtcagcggcg caatccccac ttcaaaggaa
    26761 acggtagctg tacgcggcgt agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc
    26821 agcagtacgc cagcgacttt accgattggc gtgctgaggc tgtcaacacc gccgccaact
    26881 tttgccagcg ccactaccgt tagtaccggc agaccaacgg cagtaatgag gaagccgaat
    26941 gccgcagtcc agacgtgttc gcctgcctgc aagccgacca ttggagggaa aataatgtta
    27001 cctgcgccga cgaacaacgc aaatgtcata aagcccagag cgatgatatc gcgcgatctt
    27061 aattgatggg tcataaatca tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt
    27121 gccaatccct ggggatgata cagcggtata tttaaacaat tttagcggga aatactccca
    27181 accaggcgcg gcgaagaata gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc
    27241 acctgaagcg caatttaaac gcttataacg ttttaaagca aggagggatc taaaaaccag
    27301 aggtatatag cgataagaaa tcaccaacca gtataaaaca ctatttccgt gaaaaagaca
    27361 tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga
    27421 ctgcaaagaa aataagccag catcggctgg cttatggcaa taaaagatga caaaggcgga
    27481 ttaatcgctg tttttggcaa ttaaacgttc cgggataaca aaactgaaac gtgttccttt
    27541 tcctactgta ctctcaatat tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat
    27601 cgctaaccct aatccgctac cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa
    27661 acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc ggtccgttat cttcaacgct
    27721 aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg atatgcgtgc cttctggagt
    27781 atgattcacg gcgttataga ccaggttcga aatcgcactg cgtagctgat cttcgttgcc
    27841 agacactttg aggccgttat ctatctcaaa ggtaaatgtc tgttttttct gactcagagt
    27901 ctgagcctca cgctcaacaa cgcgcagcat catcggcaca tcaacctttt cattgagcaa
    27961 atgcgtcggt gcggcttcta ttttcgacag cgtcagcaac tgcttcacca gcccttccat
    28021 ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg
    28081 ctgctcatcc atcatctcca ggtaaccctg taacacggtc aatggcgtac gtaactcatg
    28141 gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt tgatgcattt gcgtgacatc
    28201 acgcgccacc atcagcaact gtttgtgggt ataaggcatg acgcgaattt ccagatgccg
    28261 cccggtgttg agcaccagat tgagcgggcg agaaaaatca cgcgttttca gatattgcgt
    28321 aaactccggg taacgcagca ggttaaggat gttctgcccg ttatcttccg gccagcgcaa
    28381 accaagaatt tgttgcgcca gaccgttaca ccagaaaata ccgccctctt cagtggtcag
    28441 caccactgcg tcgggcagcg actccgcgcc gctacgaaag cgtttaatca gattgcccag
    28501 ttcacggcgg cgttttttat ttcgcagctg catctggtgt aagccgtata gtagcggttc
    28561 ccagctacca cgccccggtg gcggggtcat actacgatcc acccacagcc accatgaaag
    28621 gcgcaataaa ttccagaaat gccagataag cagtcccgtt accgatgcca gcaaaaacca
    28681 gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg aagcagcaaa gtagcagctc
    28741 cagcaccagc cttttccacg acagccgttc cagcacgtaa gatactccag ttaagaaatc
    28801 ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc
    28861 tgtaccgcgc acggtctgca ccatgcggtc atgcccgcct ggctccagtg ctttacgtag
    28921 gcgacgaatg tggacatcga ccgtacgatc ttccacatac acgttagttc cccagacgtg
    28981 gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag
    29041 tttaaattct gtcggcccca tctccagcgg ctcttcgccc gccatcactc ggtgagatgt
    29101 cgggtcgaga cttaatccct gcatctcaat cacctcttcc accgccattg gcgaaatacg
    29161 gcgcattacc gctttgattc gcgccaccag ctccttcggc gaaaacggct tggtgatata
    29221 gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa
    29281 catcaccact ggaatatccc gggtcatcga ctcgcgcttg aggtgtttga tgaactggat
    29341 accggagccg ccaggtaaca tccagtcaag gagaattaaa tccggccagg gttcattcag
    29401 ttgattcaca gcactgtcat aatcttccgc ttcgaccggc tgaaagccat tttgttcgag
    29461 cacgaagcag accatttcgc gaattggagc ttcatcttct acgaccagaa tacgtctcgc
    29521 catgatttgc cctgttgtaa taaataggtt gcgatcatta atgcgacgtc attatgcgtc
    29581 agatttatga cagatttatg aaaagctcgt cgcacatatc ttcaggttat tgatttccgt
    29641 ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa tcgcgcccat gctttttcgc
    29701 cagggaatcg ttatgcgcat ccttcacacc tcagactggc atctcggcca gaacttctac
    29761 agtaaaagcc gcgaagctga acatcaggct tttcttgact ggctgctgga aacagcacaa
    29821 acccatcagg tggatgcgat tattgttgct ggtgatgttt tcgataccgg ctcgccgccc
    29881 agttacgccc gcacgttata caaccgtttt gttgtcaatt tacagcaaac tggctgtcat
    29941 ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc
    30001 atggcgttcc tcaatacgac cgtggtcgcc agcgccggac atgcgccgca aatcttgcct
    30061 cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc cgtttttacg tccgcgtgac
    30121 attattacca gccaggcggg gcttaacggt attgaaaaac agcagcattt actggcagcg
    30181 attaccgatt attaccaaca acactatgcc gatgcctgca aactgcgcgg cgatcagcct
    30241 ctgcccatca tcgccacggg acacttaacg accgtgggtg ccagtaaaag tgacgccgtg
    30301 cgtgacattt atattggcac gctggacgcg tttccagcac aaaactttcc accagccgac
    30361 tacatcgcgc tcgggcatat tcaccgcgca cagattattg gcggcatgga acatgttcgc
    30421 tattgcggct ctcccattcc actgagtttt gatgaatgcg gaaagagtaa atatgtccat
    30481 ctggtgacat tttcaaacgg caaattagag agcgtggaaa acctgaacgt accggtaacg
    30541 caacccatgg cggtgctgaa aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg
    30601 cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg aaatcactac tgatgagtat
    30661 ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat cattgcctgt cgaagtattg
    30721 ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc
    30781 ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc tggcactgga agaactggat
    30841 gaatcgcagc agcaacgtct gcagcatctt ttcaccacga cgttgcatac cctcgccgga
    30901 gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg
    30961 aatggaagat tgatttcacc cgcgagccgt tcgccagcaa cgggctgttt gctattaccg
    31021 gcccaacagg tgcggggaaa accaccctgc tggacgccat ttgtctggcg ctgtatcacg
    31081 aaactccgcg tctctctaac gtttcacaat cgcaaaatga tctcatgacc cgcgataccg
    31141 ccgaatgtct ggcggaggtg gagtttgaag tgaaaggtga agcgtaccgt gcattctgga
    31201 gccagaatcg ggcgcgtaac caacccgacg gtaatttgca ggtgccacgc gtagagctgg
    31261 cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa agataagctg gaactgacag
    31321 cgacgttaac cgggctggat tacgggcgct tcacccgttc gatgctgctt tcgcaggggc
    31381 aatttgctgc cttcctgaat gccaaaccca aagaacgcgc ggaattgctc gaggagttaa
    31441 ccggcactga aatctacggg caaatctcgg cgatggtttt tgagcagcac aaatcggccc
    31501 gcacagagct ggagaagctg caagcgcaga ccagcggcgt cgcgttgctc acgccggaac
    31561 aagtgcaatc gctgacagcg agtttgcagg tacttactga cgaagaaaaa cagttactta
    31621 ccgcgcagca gcaagaacaa caatcactaa actggttaac gcgtctggac gaattgcagc
    31681 aagaagccag ccgccgtcag caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc
    31741 aacctcaact ggcggcgctc agtctggcac aaccggcacg aaatcttcgt ccgcactggg
    31801 aacgcatcgc agaacacagc gcggcgctgg cgcatactcg ccagcagatt gaagaagtaa
    31861 atactcgctt acagagcaca atggcgcttc gcgcgagcat tcgccaccac gcggcgaatc
    31921 agtcagcaga attacagcag cagcaacaaa gcctgaatac ctggttacag gaacacgacc
    31981 gcttccgtca gtggaacaac gaactggcgg gttggcgtgc gcagttctcc caacaaacca
    32041 gcgatcgcga gcatctgcgg caatggcagc aacagttaac ccatgctgag caaaaactta
    32101 atacgcttgc ggcgatcacg ttgacgttaa ccgccgatga agttgctagc gccctggcgc
    32161 aacatgccga gcaacgccca ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc
    32221 cccaacaaaa acgtctggcg cagttacagg tcgctatcca gaatgtcacg caagaacaga
    32281 cgcaacgtaa cgccgcactt aacgaaatgc gccagcgtta taaagaaaag acgcagcaac
    32341 ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat caaaacgctg gaagctcaac
    32401 gtgcacagtt acaggcgggt cagccttgtc cactttgtgg ttccaccagc cacccggcgg
    32461 tcgaggcgta tcaggcgctg gagcctggcg ttaatcagtc tcgattactg gcgctggaaa
    32521 acgaagttaa aaagctcggc gaagaaggtg cggcgctgcg cgggcaactg gatgcattaa
    32581 cgaagcagct tcagcgcgat gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac
    32641 ttactcaaca atggcaagcc gtcacggcca gcctcaatat caccttgcag ccacaggacg
    32701 atattcaacc gtggcttgat gcacaagatg agcacgaacg ccagctgcgg ttactcagcc
    32761 aacgacatga attacaaggg cagattgccg cgcataatca gcaaattatc cagtatcaac
    32821 agcaaattga acaacgccag cagcagctct taacggcatt ggcgggttat gcactgacat
    32881 tgccacagga agatgaagaa gagagctggt tggcgacacg tcagcaagaa gcgcagagct
    32941 ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg tattcagcag ctgacgccga
    33001 ttctggaaac gttgccgcaa agtgatgatc tcccgcacag cgaagaaact gtggcgctgg
    33061 ataactggcg gcaggttcat gaacaatgtc tcgcattaca cagccagcag cagacgttac
    33121 agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc ccaggcgcag tttgacaccg
    33181 cgctacaggc cagcgtcttt gacgatcagc aggcgttcct tgcggcgcta atggatgaac
    33241 aaacactaac gcagctggaa cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc
    33301 aaacgctggt cactcagaca gcagaaacgc tggcacagca tcaacaacac cgacctgacg
    33361 ggttggctct cactgtgacg gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa
    33421 agttgcgtga aaacaccacg agtcaaggcg agattcgcca gcagctgaag caggatgcag
    33481 ataaccgtca gcaacaacaa accttaatgc agcaaattgc tcaaatgacg cagcaggttg
    33541 aggactgggg atatctgaat tcgctaatag gttccaaaga gggcgataaa ttccgcaagt
    33601 ttgcccaggg gctgacgctg gataatttag tccatctcgc taatcagcaa cttacccggc
    33661 tgcacgggcg ctatctgtta cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg
    33721 atacctggca ggcagatgcg gtacgcgata cccgtaccct ttccggcggc gaaagtttcc
    33781 tcgttagtct ggcgctggcg ctggcgcttt cggatctggt cagccataaa acacgtattg
    33841 actcgctgtt ccttgatgaa ggttttggca cgctggatag cgaaacgctg gataccgccc
    33901 ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat cggtgtgatt agccacgtag
    33961 aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa aaagatcaac ggcctaggct
    34021 acagcaaact ggaaagtacg tttgcagtga aataactatt cagcaggata atgaatacag
    34081 aggggcgaat tatctcttgg ccttgctggt cgttatcctg caagctatca ctttattggc
    34141 tacggtgatt ggtagccgtt ctggtagttg tgatggtggt atgaaaaaag tcattttatc
    34201 tttggctctg ggcacgtttg gtttggggat ggccgaattt ggcattatgg gcgtgctcac
    34261 ggagctggcg cataacgtag gaatttcgat tcctgctgcc gggcatatga tctcgtatta
    34321 tgcgctggga gtggtggtcg gtgcgccaat catcgcactc ttttccagcc gctactcact
    34381 caaacatatc ttgttgtttc tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct
    34441 ctcttcgtct tacctgatgc ttgccattgg tcgcctggtt tccggctttc cgcatggcgc
    34501 attttttggc gtcggcgcga tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc
    34561 cgccgtggcg gggatggttt ccgggatgac agtcgccaat ttgctgggca ttccgctggg
    34621 aacgtattta agtcaggaat ttagctggcg ttacaccttt ttattgatcg ctgtttttaa
    34681 tattgcggtg atggcatcgg tctatttttg ggtgccggat attcgcgacg aggcgaaagg
    34741 aaagctgcgc gaacaatttc actttttacg cagcccggcc ccgtggttaa ttttcgccgc
    34801 cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc catacatgat
    34861 gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag ttgggctagg
    34921 gatggtgttg ggaaatatgc taagtggcag gatttcagga cgttattcac cactgcgcat
    34981 tgcagcagtg actgacttta taattgtact ggcactgctg atgctctttt tctgcggcgg
    35041 catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat ttgccctttc
    35101 agcacctctg caaatattat tactacaaaa tgccaaaggc ggagagttat taggtgccgc
    35161 aggtgggcaa atagcgttta accttggtag cgccgtcggc gcatattgcg gtggtatgat
    35221 gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgc tttcgtttgc
    35281 tgcgatgtcg tcattgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatagtcc
    35341 ggtgctggcg aaaccactgg ggtagattgc agtcacgttg cgcgttgctt attgccggat
    35401 gcggcgtgaa cgccttatcc gccctacgcg gttccggcac attttgtagg cctgataaga
    35461 cgcggcaagc gtcgcatcaa gcatgatgcg ccaattgcct acgtttttta ctcttgtggc
    35521 cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt acgcaccggc
    35581 gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt ttgatataaa
    35641 cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg
    35701 acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc
    35761 ggatcgcttt cttcaaccag gcggataatt tcactgcctt tcagcgcatg tccgctcaaa
    35821 cgacgataat cagtcgcgaa tcccgtgccc gaaataaagg tttcaataca accttgttta
    35881 ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc
    35941 ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgcccg cccattgaat
    36001 gccacgcccg cgccgcatcc cgtgccgata atcacggcaa atactgtctg cgctcccgct
    36061 gccgcgccat ctactgcttc tgaaaccgcc agacagttgg cgtcatttgc cagccgcact
    36121 tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa
    36181 ttggcattct tcaccacacc ggtgtaaggc gaaattgagc caggaatgcc catacctacc
    36241 gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata
    36301 gtttgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca
    36361 tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat acgcacggta
    36421 ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa
    36481 ggccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc
    36541 atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca
    36601 gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg
    36661 gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc
    36721 aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc tccggtgatt
    36781 aaacaggcgc ttgaagcgaa aatcgccaaa ctggaagcgg aacaggcgcg taagctgaag
    36841 aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc gcgtgctttc
    36901 agccgtttta gccagacaat gatgtggatc gacacggtta acggtttgat tatggtggac
    36961 tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg
    37021 ttaccggttg taccattgag catggaaaac ccgattgaac tgacgctgac cgaatgggtt
    37081 cgctccggta gtgcggcaca gggcttccag ctgcttgatg aagccgagct gaaatcgttg
    37141 ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc
    37201 aatcacattg aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt
    37261 cagtttgtga tgtgcgacga tggttcgctt aagcgtctga agttctgcga cgagctgcgc
    37321 gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc
    37381 ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa
    37441 gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc
    37501 cggcgtacga agccacacca ggcatataat tattcgctac ggcgggcaat aatttttagc
    37561 gcagcaatat tatgcgtttt atgctgtaac tgatctaatc gcctcatccc tattcgcctg
    37621 tcagcagcac gcagaatatt cactaacccc catttatcgc tggtaaattg ttttgttaac
    37681 aactccgctt ttggggtgtc aatatattca cgcaataaat cagaaattga acaaatatcc
    37741 gtcaggtaag gataccagca agtttcaccg tgaaaattac gcactccacc gttacctgca
    37801 ataaagtctt tcgggtaatc ccagatcacg tttttatcca acgtgatcca ataacgcggt
    37861 aaatcggtgc ttccaccatt ttggctgcgc atgggataac gggtacagtg aatttgcagg
    37921 ttgatcgttg gcgtcagtaa gtcatagatc tcccgctgta acttgctcca gggggcgttg
    37981 tgtcatcagt tccgcaaaaa atacctggag atcttcac
//
LOCUS       DAMZII010000035        37860 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZII010000035 DAMZII010000000
VERSION     DAMZII010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.035"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="POA77_001710"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="POA77_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HDI6365159.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAVAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="POA77_001711"
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="POA77_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HDI6365160.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPETTHQTRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPQGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="POA77_001712"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="POA77_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HDI6365161.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="POA77_001713"
     CDS             2861..3529
                     /locus_tag="POA77_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HDI6365162.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVSSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="POA77_001714"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="POA77_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HDI6365163.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="POA77_001715"
     CDS             4072..5904
                     /locus_tag="POA77_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HDI6365164.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPGGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLISWHRELAHGK"
     gene            5894..6244
                     /locus_tag="POA77_001716"
     CDS             5894..6244
                     /locus_tag="POA77_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HDI6365165.1"
                     /translation="MESKEHTPAIVVTEIGDCISTWNEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWQAARKSPLLVGIACDREKLIVHYKNLPASAPLFTLMYQQDNHTRRSIGNNA
                     ARLVKGIPFRECHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="POA77_001717"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="POA77_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HDI6365166.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="POA77_001718"
     CDS             6563..6985
                     /locus_tag="POA77_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDI6365167.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="POA77_001719"
     CDS             6988..7620
                     /locus_tag="POA77_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDI6365168.1"
                     /translation="MDKQTLQETVNQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="POA77_001720"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="POA77_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HDI6365169.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /locus_tag="POA77_001721"
     CDS             8112..8387
                     /locus_tag="POA77_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDI6365170.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRISADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="POA77_001722"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="POA77_001722"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HDI6365171.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHIPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="POA77_001723"
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="POA77_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HDI6365172.1"
                     /translation="MNNSELETLIRNILREQLVPATPRTQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNYEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDANDSWVTCEQLMPMLPIVKVNDFDSALTLALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10799..11911
                     /locus_tag="POA77_001724"
     CDS             10799..11911
                     /locus_tag="POA77_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDI6365173.1"
                     /translation="MNTFSLQTRLYSGPGSLKVISRFSHKHIWIICDVFLARSPLINT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     IVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYARLAKTCQLCPDNANDTASLNALIQHIEQLKTTCKLPTLANALKEKKAEWS
                     LCIPDMVQAALADATLRTNPRAADVSAIAELLEELL"
     gene            11908..13251
                     /locus_tag="POA77_001725"
     CDS             11908..13251
                     /locus_tag="POA77_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HDI6365174.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRLQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13254..13808
                     /gene="pduT"
                     /locus_tag="POA77_001726"
     CDS             13254..13808
                     /gene="pduT"
                     /locus_tag="POA77_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HDI6365175.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13808..14158
                     /gene="pduU"
                     /locus_tag="POA77_001727"
     CDS             13808..14158
                     /gene="pduU"
                     /locus_tag="POA77_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HDI6365176.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14163..14606
                     /locus_tag="POA77_001728"
     CDS             14163..14606
                     /locus_tag="POA77_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDI6365177.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPIIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTNAVNNHGVTELFALLHTEDVCR"
     gene            complement(14707..15036)
                     /locus_tag="POA77_001729"
     CDS             complement(14707..15036)
                     /locus_tag="POA77_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDI6365178.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15208..16266)
                     /locus_tag="POA77_001730"
     CDS             complement(15208..16266)
                     /locus_tag="POA77_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6365179.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="POA77_001731"
     CDS             complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="POA77_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDI6365180.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="POA77_001732"
     CDS             complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="POA77_001732"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDI6365181.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18431..19858
                     /gene="sbcB"
                     /locus_tag="POA77_001733"
     CDS             18431..19858
                     /gene="sbcB"
                     /locus_tag="POA77_001733"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDI6365182.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAIPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19901..20128)
                     /locus_tag="POA77_001734"
     CDS             complement(19901..20128)
                     /locus_tag="POA77_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI6365183.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20142..21200)
                     /gene="yeeE"
                     /locus_tag="POA77_001735"
     CDS             complement(20142..21200)
                     /gene="yeeE"
                     /locus_tag="POA77_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDI6365184.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="POA77_001736"
     CDS             complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="POA77_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDI6365185.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="POA77_001737"
     CDS             complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="POA77_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDI6365186.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23004..23933)
                     /locus_tag="POA77_001738"
     CDS             complement(23004..23933)
                     /locus_tag="POA77_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6365187.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23979..24803)
                     /locus_tag="POA77_001739"
     CDS             complement(23979..24803)
                     /locus_tag="POA77_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6365188.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            25230..25280
                     /gene="hisL"
                     /locus_tag="POA77_001740"
     CDS             25230..25280
                     /gene="hisL"
                     /locus_tag="POA77_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDI6365189.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25256..25379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25426..26325
                     /gene="hisG"
                     /locus_tag="POA77_001741"
     CDS             25426..26325
                     /gene="hisG"
                     /locus_tag="POA77_001741"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI6365190.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKELGASSILVLPIEKMME"
     gene            26331..27635
                     /gene="hisD"
                     /locus_tag="POA77_001742"
     CDS             26331..27635
                     /gene="hisD"
                     /locus_tag="POA77_001742"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI6365191.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARVLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            27632..28702
                     /gene="hisC"
                     /locus_tag="POA77_001743"
     CDS             27632..28702
                     /gene="hisC"
                     /locus_tag="POA77_001743"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI6365192.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPSAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLASWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            28702..29769
                     /gene="hisB"
                     /locus_tag="POA77_001744"
     CDS             28702..29769
                     /gene="hisB"
                     /locus_tag="POA77_001744"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDI6365193.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGLQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            29769..30359
                     /gene="hisH"
                     /locus_tag="POA77_001745"
     CDS             29769..30359
                     /gene="hisH"
                     /locus_tag="POA77_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI6365194.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30359..31096
                     /gene="hisA"
                     /locus_tag="POA77_001746"
     CDS             30359..31096
                     /gene="hisA"
                     /locus_tag="POA77_001746"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI6365195.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31078..31854
                     /gene="hisF"
                     /locus_tag="POA77_001747"
     CDS             31078..31854
                     /gene="hisF"
                     /locus_tag="POA77_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI6365196.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDETRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            31848..32459
                     /locus_tag="POA77_001748"
     CDS             31848..32459
                     /locus_tag="POA77_001748"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDI6365197.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLGERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32555..33532)
                     /gene="wzzB"
                     /locus_tag="POA77_001749"
                     /pseudo
     CDS             complement(32555..33532)
                     /gene="wzzB"
                     /locus_tag="POA77_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
     gene            complement(33679..33771)
                     /locus_tag="POA77_001750"
                     /pseudo
     CDS             complement(33679..33771)
                     /locus_tag="POA77_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            33941..34945
                     /locus_tag="POA77_001751"
     CDS             33941..34945
                     /locus_tag="POA77_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HDI6365198.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFVDERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(35011..36177)
                     /gene="ugd"
                     /locus_tag="POA77_001752"
     CDS             complement(35011..36177)
                     /gene="ugd"
                     /locus_tag="POA77_001752"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDI6365199.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKELNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAQRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(36426..37832)
                     /gene="gndA"
                     /locus_tag="POA77_001753"
     CDS             complement(36426..37832)
                     /gene="gndA"
                     /locus_tag="POA77_001753"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI6365200.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         9777 a         9750 c         9398 g         8935 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcaggtgcc gtagctgcgc gtaacgtcgg tgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccggaaa ccactcatca aacacgagag acggctatgg cagaaaaaag
      481 ctgcacgtta acggaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aaacgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgg gatactaaag gcggtgccgg
      721 acatggctca ttgatcctct taggtggcaa cgatgtttct gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 acagggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaagg
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cgcccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcatgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccgatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggc tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccctggcg caaggcggtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtctc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtgattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggcggaaa ccaccggaat taaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggctcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcttggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaaa
     5461 tacccgttgg caaaagtaga aagcctgttc catttacgcc atgaagacgg aagcgttcag
     5521 ttcttcccta ctccgctgtc ccctcatgta tttgcccgcg tttgtgtggt gaaacctgac
     5581 gaactggtgc ctattcccgg cgatttaaca ctggaaaaag tacgtgctgt tcgccgcagt
     5641 gcgaaagagc gcgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagtccaggc
     5701 ggaaatattc gcgatattcc gttcgtcgtt ctggtgggcg gttcttccct ggatttcgaa
     5761 gtcccgcagt tagtcaccga tgcattggcg cattatcgat tggtcgccgg acgggggaat
     5821 atccgtggta gtgaaggccc gcgcaatgcg gtagctaccg ggctactgat ttcctggcac
     5881 agggagttgg ctcatggaaa gtaaagaaca tacgcccgct attgtcgtta cggaaatagg
     5941 tgactgtatc agtacatgga atgaggtgct tcttggcatt gaagaggaag gcatcccctt
     6001 tcgcattcaa catatcccgt caggtgaagt catcgatagc gcctggcagg ctgctcgtaa
     6061 gtcccccctg ttggttggca tagcctgcga ccgggaaaag ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gatgtatcaa caagataacc atacccggcg
     6181 cagcatcggc aataacgctg cccgcctggt aaaaggcatt cccttcaggg aatgccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg caactggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cagtgatggt ccgtggcgat gttggcgcag
     6421 taaaagccgc cgttgacgca ggaagcgccg ccgcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttact aaggagcaca gcgtgaagca atcactggga ttacttgaag tcagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatagcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgaac
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcaa cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgcg gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagtca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gttattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaac tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcagcgattc agatacacga agcgttagcc tggggattgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat tgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcaaccgat ctaccttcat cgtgatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtttta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaact tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccggat cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agtgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc tgcgctggaa aacgcgatcg atagcgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcgagccg
     8761 catgcatttc gcccgcacag tggctcgacg ggctgaaaga cgtctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgtctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacatacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaaac ttgctgatac cgtgcaaccc ggcgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggca aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgctcaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgcgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgttgatgaa gcggtctgcg cagcacacca ggccttttta
     9541 cgctttcaac agtgtccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcacttgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacctgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccaatg atagttgggt tacttgtgaa caactgatgc caatgctgcc cattgtgaag
    10501 gtcaacgatt ttgatagcgc tctcaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactcgca gaacgtctca cggctcaacc ttgccgcacg cgttatgcaa
    10621 acctcaatct ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgacc ggagaaggga caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggaccg ggaagtctca aggtaattag
    10861 ccgtttctct cataaacaca tctggattat ttgcgacgtc tttctggcac gctcaccact
    10921 cattaacacg ttgcatcagg cgctgcccga cagtaatcgt cttagtattt ttagcgaaat
    10981 aacgcccgac ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgtg gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcgattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtaccggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctctgat
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcgctgccaa
    11641 acgctacgcc cggctggcga aaacgtgcca attatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaaa acaacctgta agctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca ttatgcatac cagatatggt
    11821 tcaggcggca ctggcggatg caacgttgcg taccaacccg cgtgcggctg atgtctccgc
    11881 gattgcagaa ctgctcgagg agttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagttca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgttgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 ccaatgttga aagtggatca acagttgatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagagaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttacccagc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tagtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa accaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gattgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcggt taactaccaa
    12781 cagctcgcca cgccgcaact gttactgaca gcgctcacct gttcagagtg caacgtctgc
    12841 gccagtgtcg cctgtccggt aggaatttca ccaatgcgca taaaccgcct gcttaagcag
    12901 gaattacgcg cacaaaatct ccgctacgaa ggtgccttaa atcctgcaga tcccatggca
    12961 aattatcgac tgattcccgt aaaacggctg gtaaccagac ttggcttaac cccgtggtat
    13021 caggatgcgc cattgtctga acaggtgcct cagcccgaaa aagtgacgtt attgttacgc
    13081 cagcacatcg gtgccagcgc aattgcgtgt gtacaaaaag gcgatcgcgt cgttcatggg
    13141 caatgtgtag gtcaaattcc acacggcaca ttaggtgccc ccattcatgc cagtattgat
    13201 ggcatggtca gcgatgtgac agaaaacgcg attacgcttg tgagaggtta accatgtcga
    13261 aagcggtagg tattttagaa cttagcagta tcgcgaaagg gatggaaacc ggcgacgcca
    13321 tgctgaaaag cgccaacgtt gagttactgg tcagcaaaac gctatgcccc ggaaaatttc
    13381 tgctgatgct cggtggcgat gtgggcgcgg tgcaacaggc catcaacagc ggagcgtccc
    13441 ttgctggtga aatgctggtc gacagtcttg tcctgccgaa tattcatccc agcctccttt
    13501 ctgcaatcag cggactcaac catgttgaac agcaccgggc tgtcggtgta gtcgaaacct
    13561 ggagtgtcgc cgcctgcatt gacgctgccg attgtgctgt caaagctgca aatgtcattc
    13621 ttgttcgcgt acatatggct tttggcattg gcggtaaatg ctacatggtg gttgctggcg
    13681 atatttcaga tgttaataac gcggtctctg tcgccagcga gcgtgcgggg gaaaaaggtt
    13741 tgttggtcta tcggtcggtg attgcccgtc cacatgaagc catgtggcgt cagatggtgg
    13801 agggataatg acaactcagc acacaactga acggatgatt caggaatatg tccctggtaa
    13861 gcaggtcacg ctggcacatg tcattgctaa tccagggaag gatctgttta agaaattggg
    13921 tttaccagac accatttccg ctattggtat tctgacaatt acccccagcg aagcatctat
    13981 catcgcatgc gatatcgcca caaaatcagg cgctgtagag atcggttttc ttgatcgttt
    14041 taccggtgcc gtagtgctga ctggtgatgt ttctgcgatc gaatatgcac ttagccaggt
    14101 tacccgtacg ctgggtgaaa tgatgcgctt taccgcctgc cccattacgc ggacctgacg
    14161 ttatgcaacg tatgatgctc atcggcccca gccagtgcgg aaaaacgtca ctcacgcaat
    14221 gtctgcgtgg cgaaaatatt cattatcaga aaacgcaggc aatcgtctgg tccccgacca
    14281 ccattgatac accaggcgaa tacctcgaaa accgcagcct ctacagtgca ctgttggtca
    14341 gtgcctgcga agcggacatt atcgcgctgg tgcttaatgc ggacgcaccg tggtcacctt
    14401 tttcgccagg ctttacctcg tcaatgaatc gcccgatcat tggagtagtc accaaagcag
    14461 atttagccag catggaacaa atatctttgg tgaaatgttg gttacgggaa gccggagcgc
    14521 acaacgtgtt agtaaccaat gcggttaata accatggagt tacagaactt tttgccttgc
    14581 tgcatacaga agatgtctgt cgctaatttc gataggttat cagatgggaa tgatgactgt
    14641 gatacacaga aaagaatcaa tgagcaatga gggttgccag gcaaccctca ttgaataaaa
    14701 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
    14761 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
    14821 atagtcacct ttcatgctag cgatgatgcc ctggattgct tcaacgctca tccccggctt
    14881 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
    14941 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
    15001 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
    15061 gtcggattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
    15121 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
    15181 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
    15241 tagcaactca agctgatgtg ccgtctccat gttcagccac acataaccat agattggtgt
    15301 ttccacaacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
    15361 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
    15421 aaggctcagc agtatttgct gcatcatgtg ctgggtgtca cgaagttttt gcgcgttcaa
    15481 taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact ccagcataca
    15541 aaccagattg cgattaatgg tctggatacc ttcatatatc gattttggaa tacgagtttc
    15601 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcaaga gtttttgtag
    15661 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gataaacccg
    15721 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
    15781 ccagataccg gtaaacaaca ttgcgagtaa agagccgagg atcacatcgc cgcttcgcca
    15841 taacgccgta tcaatttcac ctgtcgggga acccacaaca attgccagcg tcaccccaat
    15901 caataatcct tgatacggtt tcttgcccag tgccagccaa ccgcaaagaa acatagccgc
    15961 cgcgcaccag actaacatca gcggtaacga aattaactcc agttgcagag cgataagacc
    16021 taatatcgaa cccaacaccg taccgccaat acgttcgaag gctcgaggga caacgttacc
    16081 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
    16141 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
    16201 atgcacaatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
    16261 acgcacgcag aaactccgta atgagattaa aagcgctcat aactcatctg tcggccagaa
    16321 ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc
    16381 gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaaagagt ggtcatcgcg
    16441 tcaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
    16501 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
    16561 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
    16621 aaatcatcac cgactacacg agctaccgcc accgcatact gaccacctga aatttctgtc
    16681 agaatgacac cctcactgtt ttcgggaagc gtaaagtaac ccggcaccgt cacgacggtg
    16741 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagat agcaacccac
    16801 tccttcggca caatattttt gctatctacc cacatcatca actgctcaaa acctttcttt
    16861 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt atcttcctgc
    16921 ttaatctcgt agttcatgac gcccccatta atactgtttt tatatacagt atagttgcaa
    16981 aattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
    17041 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcgag aaaacatgct
    17101 gccttcccca acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc
    17161 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gttctttacc
    17221 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagta ccatccagaa
    17281 ctcttgttct gttcccaggg cgatattttc tttatcgcca taccagatgc gttccgtacc
    17341 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
    17401 taattttctt gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat
    17461 gagacgacgc tgcccatcta cagccgaagc aatgaggtta aaaccggcac cagaagtatg
    17521 acccgttttc aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg
    17581 ctgggtgata ccgttccagg tgagactttt ctcactgtac atatgataaa actcgggctc
    17641 gccgtggatg atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg
    17701 tgcatccaga ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
    17761 gttgttcatc atttcaacaa actgccgttg cccaccggca atatagtcag ccagagcaac
    17821 acaagcgtca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
    17881 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca
    17941 cgcatcgcgc cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg
    18001 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
    18061 attacccgcg gtgaggatct gaccggtggt gtaatccatc agtacccagg acccggcatg
    18121 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
    18181 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
    18241 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
    18301 aatgtgacta ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
    18361 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg
    18421 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
    18481 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
    18541 tagcgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg cggatgacta
    18601 tttaccccag cctggagcag tattaattac cggtattacc ccgcaggaag cacgggcgaa
    18661 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac
    18721 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
    18781 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
    18841 gttggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
    18901 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gtattgaaca
    18961 tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg cgaaactggt
    19021 aaaaacgcgt cagccacgcc tgtttgaata tctctttacc catcgtaata aacacaaact
    19081 gatggcgttg attgatgttc cgcagatgaa acccctggtg cacgtttccg gaatgtttgg
    19141 ggcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatcctg aaaatcgcaa
    19201 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga
    19261 cacattgcgc gagcgtttat ataccgcaaa aaccgatctt ggcgataacg ccgccattcc
    19321 ggttaagctg gtgcatatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc
    19381 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc taaaaattct
    19441 gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
    19501 tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg acgccgatcg
    19561 tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
    19621 ttttgttgat aaacggattg aaaagctgtt gttcaattat cgggcgcgca acttcccggg
    19681 gacgctggat tatgccgagc agcaacgttg gctggagcac cgccgccagg tattcacacc
    19741 cgagttttta caaggctatg cagaagagat tcagatgctg gcgcagcagt atgccgacga
    19801 taaagaaaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctagtt
    19861 tgaagcctcc gccgctgata cggcggagga caatgttggg tcaggctttt tgaacggtga
    19921 tgctccacgc agcatcgcca atctgctgat aatcggtgat ggcatgtcct tcttctgccg
    19981 cccattgtgg gattgcttct gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc
    20041 cgctggccat tcctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg
    20101 tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt
    20161 gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg
    20221 agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt
    20281 ccattaccga tagagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct
    20341 aacccgctac gtactgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct
    20401 ttggctgcaa taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg
    20461 tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct
    20521 aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg
    20581 atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg
    20641 gttcggcgcg gcggtaaggt cgcgactttc agttttggct tcttcaactc tttcatcacc
    20701 acccagagcg ttatcaccag caaaacggta accagcggcc acacagacaa attaaaagtc
    20761 tcagcaatag aattatgttc agtactgtag tgctgcaagg tttgatttaa accactggca
    20821 tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg
    20881 atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt
    20941 cccagcccga agatataccc acctataaca gtaccaagcc acgggaacgc cccggcttcg
    21001 taagtcagta ggcccgcctg aattaacgca aaaaccccca cgctttgtac cgaaattgca
    21061 atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacct
    21121 gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg
    21181 cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc
    21241 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
    21301 tgaggatgtc gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt
    21361 tattattaga aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct
    21421 cttggtgacg caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat
    21481 tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag
    21541 gaacagatac tggaagtgat ctttcagcgt cttgttacgc ttctcacgga tccagaactg
    21601 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc
    21661 cgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
    21721 gttcattgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac
    21781 gccgtcacga cccattacgt acatcagacg cgcaacacct gcgtgcgccg ccataccgga
    21841 cgccagtacg gtaatggtgg agaagatcag cgcaccaacc tggaatgctt tacctgcaac
    21901 atacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
    21961 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag
    22021 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat
    22081 gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac
    22141 atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc
    22201 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
    22261 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
    22321 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcactagag cttcaaaata
    22381 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
    22441 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc
    22501 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
    22561 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
    22621 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
    22681 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
    22741 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
    22801 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
    22861 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
    22921 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
    22981 aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
    23041 acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagccct
    23101 gcggtctgtt cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
    23161 ttctcgatta acgcgccact atcgagaaac cgttgcgcca gataacgggg taaatagccg
    23221 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct
    23281 tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc
    23341 acaatcgccc gatatcgctt aataatacga cggtttaacg gctcttcttc cagagctaac
    23401 ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac
    23461 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
    23521 agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga
    23581 tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
    23641 ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
    23701 cgcacagtat gcagaacttc ccgccctttc tctaataaca tttttccggt acgggtgaat
    23761 ttagcgcggt gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga
    23821 acggtgtaac tcagagcgga cggcgtctta tagagtttgg ccgacgccgc cgcaaaactg
    23881 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc
    23941 cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc
    24001 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
    24061 ttacctttgc cgctgtccag actatttctg aactgcggag gctccagccc cagtaaacgg
    24121 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
    24181 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
    24241 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
    24301 tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt accgggtaaa
    24361 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc
    24421 ggggtagttt ctttcaccgt gccttgcgcg tcgccataga cagatgttga gctggtaaaa
    24481 ataatgcggg gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
    24541 tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag cgcatcgaca
    24601 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
    24661 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
    24721 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
    24781 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
    24841 cgctagcgta tcaaaactga caattcattc tatgaatgaa atcgaaactg ttagcaaata
    24901 taccattgcc tgatgcgctc cgcttatcag tcctacaaaa acatcaatat attgaattta
    24961 ggcaacattt gtagaccaga tatggcattc acgccgcatc cggcaggaac aaagcccact
    25021 ttagttactg aacacaatgt tcttgagggt tttaaagaca tattttcacg attacttttc
    25081 taacgtaatc actccaggcc ccctcctttc cccgctcatt cattaaacaa atccattgcc
    25141 ataaaatata taaaaaagcc cttgctttct aacgtgaaag tggtttaggt taaaagacat
    25201 cagttgaata aacattcaca gagactttta tgacacgcgt tcaatttaaa caccaccatc
    25261 atcaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt cagatcttcc
    25321 agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt
    25381 gcgcgattga taacggttca gacaggttta aagaggaata agaaaatgac agacaacact
    25441 cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg cgaattgctg
    25501 gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat ggcagaaaac
    25561 atgccgattg atattctgcg cgtgcgtgac gacgacattc ccggtctggt aatggatggc
    25621 gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct taaccgccgc
    25681 gcccagggtg aagatccacg ctactttacc ctgcgtcgtc tggatttcgg cggctgccgc
    25741 ctttcgctgg caacgccggt tgatgaagcc tgggacggcc cgctctcctt aaacggtaaa
    25801 cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa aggcatctct
    25861 tttaaatcct gcttactgaa cggttctgtt gaagtcgccc cgcgtgccgg actggcggat
    25921 gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct gcgcgaagtc
    25981 gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat ggaagaatcc
    26041 aaacagcaac tgatcgacaa actgctgacc cgtattcagg gtgtgatcca ggcgcgcgaa
    26101 tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat cgccctgctg
    26161 ccaggtgccg aacgcccaac tattctgccg ctggcgggtg accaacaacg cgtagcgatg
    26221 cacatggtca gtagcgaaac cctgttctgg gaaacaatgg aaaaactgaa agagctgggt
    26281 gccagttcaa ttctggtcct gccgattgag aagatgatgg agtgatcgcc atgagcttta
    26341 acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg ttaatgcgcc
    26401 cggcgatctc cgcttctgaa agcattaccc gcactgttaa cgatattctc gataacgtga
    26461 aagcacgcgg cgatgaggcc ctgcgggaat acagcgcgaa gtttgataaa accacggtta
    26521 ccgcactgaa ggtgtctgct gaggaaattg ccgccgctag cgaacgcctg agcgacgagc
    26581 taaaacaggc gatggcggtg gcagtaaaga atattgaaac cttccacact gcgcaaaaac
    26641 tgccgccggt agatgtagaa acgcagccag gcgtgcgttg ccagcaggtc acgcgcccgg
    26701 tcgcttcagt tgggttgtat attcctggcg gctccgcccc gctcttctca acggtattaa
    26761 tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt agtgttgtgc tcaccgccgc
    26821 cgattgccga tgagatcctt tatgcggcgc agttgtgcgg tgtgcaggac gtgtttaacg
    26881 tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg ccgaaagtgg
    26941 acaaaatctt cgggccgggt aacgcctttg tcaccgaagc gaaacgtcag gtgagccagc
    27001 gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg gtgattgctg
    27061 acagcggcgc tacgccggat ttcgtggctt ctgatttgct ttcccaggct gaacacggcc
    27121 cggactcaca ggtgatttta ctgacgcccg ctgctgatat ggcgcgtcgc gttgccgaag
    27181 ctgtcgaacg ccagttggca gaactgccgc gtgccgaaac cgcccgtcag gcactgagcg
    27241 ccagccgcct gatcgtgact aaagatttag cgcagtgcgt agagatctcc aaccagtacg
    27301 gcccggagca cctgatcatt cagacccgca acgcccgcgt actggtcgat ggcattacca
    27361 gtgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggtgat tacgcctccg
    27421 gcaccaacca cgttctgccg acttacggtt acaccgccac ctgttccagc ctcgggctgg
    27481 cagatttcca gaagcgcatg accgtgcagg aactgtcgaa agaaggtttc tccgcgctgg
    27541 cttcgaccat tgaaacattg gccgccgccg agcgcctgac cgcccacaaa aatgccgtta
    27601 ctttgcgtgt taacgccctt aaggagcaag catgagcacc gtgactatta ccgatttagc
    27661 gcgtgaaaac gtccgcaacc tgacaccgta tcagtcagcg cgtcgtctgg gtggtaacgg
    27721 cgatgtctgg ctgaacgcca acgaataccc ctctgccgtg gagtttcagc ttactcagca
    27781 aacgctcaac cgctacccgg aatgccagcc gaaagcggtg attgaaaatt acgcgcaata
    27841 tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag gtattgaact
    27901 actgattcgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc cgccaacgta
    27961 cggcatgtac agcgtcagcg ccgaaaccat tggcgtcgag tgccgcacag tgccgacgct
    28021 ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg taaaagtggt
    28081 ctatgtttgc agccccaaca acccaaccgg gcaactgatc aatccgcagg attttcgcac
    28141 tctgctggag ttaacgcgcg gtaaggcgat tgtggttgcc gatgaagcct atattgagtt
    28201 ttgcccgcag gcctcgctgg cgagctggct ggcggaatat ccgcacctgg ctattttgcg
    28261 cacactttcg aaagcttttg ctctggcggg gcttcgttgc ggatttacgc tggcaaacga
    28321 agaggtcatc aacctgctga tgaaagtgat cgccccctac ccgctctcga cgccggttgc
    28381 cgacattgcg gcccaggcgt taagcccgca gggaatcgtc gctatgcgcg aacgagtgac
    28441 gcaaattatt gcagaacgcg aatacctgat tgccgcactg aaagagatcc cctgcgtaga
    28501 gcaggttttc gactccgaaa ccaactacat tctggcgcgc tttaaagcct ccagcgcagt
    28561 gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac aaccctcttt
    28621 aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg tcattgacgc
    28681 cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg cgatggaacc
    28741 ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact cgcctttgaa
    28801 ccgggcgtga tcccggagct gctaaagctg caaaaagcgg gctacaagct ggtgatgatc
    28861 actaatcagg atggtcttgg aacacaaagt ttcccgcagg cggatttcga tggcccgcac
    28921 aacctgatga tgcagatctt cacctcgcaa ggcctacagt ttgatgaagt gctgatttgt
    28981 ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact ggtggagcgt
    29041 tatctggctg agcaagcgat ggatcgcgcc aacagttatg tgattggcga tcgcgcgacc
    29101 gacattcaac tggcggaaaa catgggcatt actggtttac gctacgaccg cgaaaccctg
    29161 aactggccaa tgattggcga gcagctcact aaacgagacc gttacgccca tgtggtgcgc
    29221 aacaccaaag agacgcaaat tgacgtccag gtgtggctgg atcgtgaagg tggcagcaag
    29281 attaataccg gcgttggctt ctttgatcac atgctggatc agatcgccac ccacggcggt
    29341 ttccgtatgg aaatcaacgt caaaggcgac ctctatatcg acgatcacca caccgtcgaa
    29401 gataccggcc tggcgctggg tgaagcgtta aaaattgccc ttggcgacaa acgcggtatt
    29461 tgccgctttg gttttgtgct accgatggac gaatgccttg cccgctgcgc gctggatatc
    29521 tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgcgt gggcgatctc
    29581 agcaccgaaa tgatcgagca cttcttccgt tcactctcat acaccatggg cgtgacgcta
    29641 cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag agagcctgtt caaagccttt
    29701 ggtcgcaccc tgcgccaggc catccgcgtg gaaggcgaca ccctgccctc gtcgaaagga
    29761 gtgctgtaat gaacgtggtg atccttgata ccggctgcgc caatctgaac tcggtgaagt
    29821 cagccattgc gcgccacggt tatgaaccaa aagtcagccg tgacccggac gtcgtgttgc
    29881 tggccgataa actattttta cccggcgttg gcactgcgca agcagcgatg gatcaggtgc
    29941 gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg ggcatctgct
    30001 tagggatgca actgctgggg cggcgcagcg aagagagcaa cggcgtcgat ttgctaggca
    30061 tcatcgacga agacgtgccg aaaatgaccg actttggtct accgctgccc catatgggct
    30121 ggaaccgcgt ttacccgcag gcaggcaacc gcctgtttca ggggattgaa gacggcgcgt
    30181 acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc gcccagtgta
    30241 attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc gtacagttcc
    30301 acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg gagatgtgat
    30361 gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc agggcgatta
    30421 cggcaaacag cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt acgccacgca
    30481 gggtgccgaa gtgttgcatc tggtggatct gaccggggca aaagatccgg ctaaacgtca
    30541 aatcccgctg attaaaaccc tggtggcggg agttaacgtt ccggtgcagg ttggcggcgg
    30601 cgtgcgtagc gaagaagacg tggcggcgtt actggaagcg ggcgttgcgc gcgtggtggt
    30661 cggctccacc gcggtgaaat caccagaaat ggtgaaaggc tggtttgaac gtttcggtgc
    30721 cgatgcctta gtgctggcgc tggatgtccg tattgacgag caaggcaaca agcaggtggc
    30781 agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg aaacctatct
    30841 gcccgtcggc ctgaaacatg tgctgtgtac cgatatctcg cgcgacggca cgctggcagg
    30901 ctctaacgtc tctttatatg aagaagtgtg cgccagatat ccgcaggtgg catttcagtc
    30961 ctccggcggt attggcgaca ttgatgatgt ggcggccctg cgtggcactg gtgtgcgcgg
    31021 cgtaatagtt ggtcgggcat tactggaagg taaattcacc gtgaaggagg ccatcgcatg
    31081 ctggcaaaac gcataattcc gtgtctcgac gttcgtgatg gtcaggtggt gaaaggcgta
    31141 cagtttcgca accatgaaat cattggcgat atcgtgccgc tggcaaaacg ctacgccgaa
    31201 gaaggtgcag acgaactggt gttctacgat atcaccgctt ccagcgatgg ccgtgtggta
    31261 gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttctg cgtggcgggt
    31321 gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga taaaatttcc
    31381 atcaactccc ctgcgctggc ggatccgacg ctaattactc gcctggcgga tcgctttggc
    31441 gtgcagtgta ttgtggtcgg tattgatacc tggtacgacg ctgaaaccgg taaatatcat
    31501 gtgaatcaat ataccggcga tgaaacccgc acccgcgtca ctcagtggga aacgctcgac
    31561 tgggtacagg aagtgcaaaa acgtggagcc ggagaaatcg tcctcaacat gatgaatcag
    31621 gacggcgtgc gtaacggtta cgacctcgag cagctgaaaa aagtacgtga agtttgccac
    31681 gtcccgctga ttgcctccgg tggcgcgggg accatggaac acttcctcga agccttccgt
    31741 gatgccgacg ttgacggcgc gctggcagct tccgtattcc acaaacaaat aatcaatatt
    31801 ggtgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg ttaacagaac
    31861 aacaacgtcg cgaactggac tgggaaaaaa ccgacggact gatgccggtg attgtgcaac
    31921 acgcggtatc cggcgaagtg ctaatgctgg gctatatgaa cccggaagcc ttagacaaaa
    31981 ccatcgaaag cggcaaagtc accttcttct cgcgcactaa acagcgactg tggaccaaag
    32041 gcgaaacgtc gggcaatttt ctcaatgtgg tgagtattgc cccggactgc gacaacgaca
    32101 ctttactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc agcagctgct
    32161 tcggcgatac cgctcaccag tggctgttcc tatatcaact ggaacaactg ctcggcgaac
    32221 gcaaatctgc cgacccggaa acctcttaca ctgccaaact gtatgccagc ggcaccaaac
    32281 gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggccgcg actgtacatg
    32341 accgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg gtgctgttgc
    32401 aggatcagga tctggactta acgacggtga ttgagaacct gcgtaaacgg catcagtgag
    32461 ttgcggggta agcggatgcg atgttgttgt cgcatccggc aaaaaaacgg gcaaggtgtc
    32521 accaccctgc ccattttctt taaaaccgaa aagattactt cgcgttgtaa ttacgcagag
    32581 cgttacgccc caacacaatc cccgcgccaa ccatgcagcc cagcagcacc gccagaatca
    32641 aggtaattgc ctttttcggg ctatcgcgac gaataggtaa cgtcggtttc attacatagc
    32701 ggtaagcatg aatatcaagt ttatcaacgt caagattgtc gatatccagc aggttttgac
    32761 gagtctgata gtagtttgat gagaacacca acggacgagt cgcctcgtgc ttaatcatcg
    32821 actccagcgc ttcgctcccc aaaaggaaca tagtatccta ggttacgtcc tgtgtctgct
    32881 gaatctgcgg ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac
    32941 ggatacgcag atctttctgc tcctgcgcaa ccacttcctg ggtcttcaga gaatcctgca
    33001 agtttttcat ttgcagcgcg atgttgtctt tcaggtcttg ttccagttct tcatttacct
    33061 ggtcgtcaac ttgctggata tactgcgcca gctgtttctg cgcaccttca ggtgtttcac
    33121 ccacataaga aaccgctaaa ggtaatgact gatttttaac ggtaggctca atggtcagtt
    33181 tttcaggttc ttcctgatta tccagcgttt ctgctaatgc agaaaatgtc gtgctgaaac
    33241 gaccaattaa catcgactgg atctcagaca ctttcggcgc tgccgaacca taaatcacat
    33301 ttatggcatt gttataacca gcgatttgcc ccacatcagg ttgagtaacg atagctgtag
    33361 aagtccactt ttctttggca acggccagat accctatagc cagggcaata gcgacaatga
    33421 cagagatgat aattgtcatc ttgccacgcc acaactgcac cagtaaatca atcaaatcaa
    33481 tctgttccgg gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc
    33541 taacggacga aataatttta agtcgccata gtttagcgat aatcgccatg aatgctatag
    33601 gataaatgat aaaaataatg aattattaca aagggagcat aagagaatca gggcgaaaat
    33661 cgccctgata acaggatgtt agtcactgcc aaagagatcg cgggtgtaga ccttatctgc
    33721 cacatcctta agctcttctg ccatacggtt ggagataatg acgtcggctt gtatagtcag
    33781 cttagaacag ctttgattgt aaaatgagca cgttaccaga aaaaacagcc aggtttagaa
    33841 ctgataccga ttatcttttt ttcttgtcta acggtatgat ttaactttca gttatgctag
    33901 atgaaaattg gctatattct agcctgagcg aggattataa atgaaatttc tggttacggg
    33961 agctgctggc tttatcggtt tctatgtaag taaacggctt cttgcagctg gtcatcaggt
    34021 tgtaggcatt gacaacttaa atgattatta cgatgtcaac ctcaaacaag cacgacttga
    34081 tttactcaag cacgacaatt tcagttttta taaaattgac ctggccgatc gcgagaaaat
    34141 ggcggcactg tttgtagacg agcggttcga tcgcgtaata aacctcgctg cgcaagccgg
    34201 tgtacgttac tcccttgaaa atcccaatgc atatgcagat gcaaacctga ttggattcct
    34261 gaacatacta gaaggatgtc gccataataa tgttcagcat ctactttacg cttcctccag
    34321 ttctgtttat ggcatgaacc gcaagatgcc tttctctaca gatgactctg tagatcatcc
    34381 tgtttcgctt tatgcagcaa ctaaaaaagc gaatgaactc atggctcata cgtattctca
    34441 tttgtatagc ttaccgacca cagggctgcg tttctttacg gtttatggtc catggggacg
    34501 tccggatatg gcattattta aattcactaa agccatgctg gaaggtaaaa gcattgatgt
    34561 ttacaacttc ggcaaaatga agcgtgactt tacttatatt gatgatattg ccgaagctat
    34621 tattcgctta caggatgtta ttccagaaaa agatccacac tgggctgtag aaacaggctc
    34681 gccagcaaca agttcagcac catatcgtgt ctataacatt ggtaatagtt cgcctgtgga
    34741 gttgatggac tatatcaatg ccttagaaga ggctctgggt attgaagcca acaagaatat
    34801 gatgcctctc caacccggtg atgtactgga aaccagtgct gatacaaaag cactgtatga
    34861 cgtaatagga ttcaaacctg aaacgtcagt taaagagggg gtaaagaact ttgtagaatg
    34921 gtatcgtaac ttctataaag tttgatttta caaaaccata agaaaaggcc ctaatatatt
    34981 agggcctttt cttagaatga aacaaaataa ttaatcattg ccaaacaagt cgcgcgtata
    35041 aactttatct gctacatcag ccagatcggc agacatacgg ttagaaataa taacatcagc
    35101 ttcttgtttg aacgcatcca gatcacgtac cacgcgcgac cggaaaaaat cgtcctcttt
    35161 catcgctggc tcataaacga ttactggcac acctttcgcc ttgattcgct tcataatacc
    35221 ctgaatcgat gaagcacgga aattgtccga accactcttc ataatcagac gatagacgcc
    35281 aacaactttc ggtttacgtg caaggataga atcggcaata aagtcttttc gcgtgcgatt
    35341 ggcatcgaca attgctgaga tcaagttatt cggtactgac tgatagtttg ccagtaactg
    35401 cttagtatct ttcggcagac aataaccacc atatccgaat gacgggttgt tgtagtgatt
    35461 accgatacgc gggtcaaggc atacaccctc aataatctgg cgggaattaa gccccagact
    35521 ttcagcgtaa ctatctaatt cattgaaata agccacacgc atcgccagat aagtattagc
    35581 aaaaagttta atcgcctcag cttcggttga gtcagtaaac aacgttggta tatcttgctt
    35641 aatggcgcct tcctgtaata acgctgcaaa acgttgagcg cgatcggatt gctcgccaat
    35701 cacaatgcgt gatgggtgta agttatcata aagtgctttc ccttcacgta aaaactccgg
    35761 cgaaaagatc acattttcaa taccaaaccg ttctttaatg gactctgtaa aaccgacagg
    35821 gatagttgat tttataatca ttacagcgtc gggattaatt tctgtcacat cacggatgac
    35881 cgcttccacg cttgaggtat taaaataatt tgtttttggg tcataatcgg taggcgtggc
    35941 aataataacg taatcggcat ttttatacgc gtcatactta tctgtcgtag cgcggaaatt
    36001 gagctcttta gtcgccagat actcttcaat ctccttatca acaatcggtg actgcttctt
    36061 gttaagcatg tccactttgg cctgaacgat atccagtgca accacttcgt ggttttgcgc
    36121 aatcagaata ccatttgaaa gaccaacata acctgttcct gaaattgtta ttttcattag
    36181 ctctgacttc ttccggttaa acatttagag tggtcattaa tccaccacca cagcttcatc
    36241 tactgcggga tttttaacgc tgatgtagat gaactgtcaa ggcagcgatc ctgctgtgcg
    36301 gctctgtatt atatcgcttt tttaatcata aattataaaa aaaggccata acctgccgct
    36361 ttgtataata aaaaagcccg gagggtttct ccgggccttg ctttgattaa ttgatttaaa
    36421 tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta
    36481 tgcgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcagaacgg
    36541 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtacc
    36601 gcataagcga cgacatcacg cagcgcctgc tggtagtcat cggcaatttg cttgaagtac
    36661 ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
    36721 aggaactgcg cacgaatgat gcagccagca cggaaaatct tcgcgatttc accgtagttc
    36781 agatcccagt tgtactcttc agacgcagcg cgtagctgag agaagccctg ggcgtaagaa
    36841 acgattttgc ccagatacag tgcacggcga actttttcga tgaactcagc cttgtcgcct
    36901 gctgactgag cttgcggacc agagagaact ttagatgcgg caacgcgctg atctttcaga
    36961 gaagagatgt atcgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
    37021 agcgcactct ggctggtcca tttaccggta cctttgttag ccgcttcatc cagaatcaca
    37081 tcaaccaggt agttaccctc ttcatctttt ttggtgaaga tatctttggt gatgtcgatc
    37141 aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccag ttcttcgttg
    37201 gaaagattca ggccaccttt aagcagagaa taggcttcag caatcagctg catatcaccg
    37261 tattcgatac cgttgtgaac catcttcaca tagtggcctg cgccatcggc accaatatag
    37321 gtaacgcacg gttcgccatc ttcagcaacg gcggcgattt tggtcaggat cggcgcaacc
    37381 agttcatagg cttctttctg cccaccaggc ataatagaag gacctttcag cgctccttct
    37441 tcaccgccgg aaacaccggt accaatgaag ttaaagcctt cggcagaaag ctcacggtta
    37501 cgacggatgg tatcctggaa gaaggtatta ccaccatcaa taatgatgtc acctttatcg
    37561 aggtatggct taagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
    37621 aggatgcgac gaggcgtttc caaagattca acaaattctt tcaccgtata gtaaggaacc
    37681 agtttcttgc ctggattttc ggcaatcact tcttccgtct tctcacggga acggttgaaa
    37741 atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc
    37801 ataccgacga cgccgatctg ttgctttgac attgtttact cctgtctgcg gggaagtagt
//
LOCUS       DAMZII010000036        37150 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZII010000036 DAMZII010000000
VERSION     DAMZII010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.036"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..3124)
                     /gene="rhsB"
                     /locus_tag="POA77_001754"
     CDS             complement(<1..3124)
                     /gene="rhsB"
                     /locus_tag="POA77_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDI6365201.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDS
                     LDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRY
                     RYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAERWRYDERGWLTDISHISEGHRVAV
                     HYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVDFTRDRLHRETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRD
                     YTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQ
                     HRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWD
                     GDRLTTIQNDR"
     gene            complement(3284..3496)
                     /gene="rpmE"
                     /locus_tag="POA77_001755"
     CDS             complement(3284..3496)
                     /gene="rpmE"
                     /locus_tag="POA77_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDI6365202.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            3699..5897
                     /gene="priA"
                     /locus_tag="POA77_001756"
     CDS             3699..5897
                     /gene="priA"
                     /locus_tag="POA77_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI6365203.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            6053..7078
                     /gene="cytR"
                     /locus_tag="POA77_001757"
     CDS             6053..7078
                     /gene="cytR"
                     /locus_tag="POA77_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDI6365204.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            7170..8129
                     /gene="ftsN"
                     /locus_tag="POA77_001758"
     CDS             7170..8129
                     /gene="ftsN"
                     /locus_tag="POA77_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDI6365205.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            8222..8752
                     /gene="hslV"
                     /locus_tag="POA77_001759"
     CDS             8222..8752
                     /gene="hslV"
                     /locus_tag="POA77_001759"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI6365206.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            8762..10093
                     /gene="hslU"
                     /locus_tag="POA77_001760"
     CDS             8762..10093
                     /gene="hslU"
                     /locus_tag="POA77_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDI6365207.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            10160..11086
                     /gene="menA"
                     /locus_tag="POA77_001761"
     CDS             10160..11086
                     /gene="menA"
                     /locus_tag="POA77_001761"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI6365208.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            11179..11664
                     /gene="rraA"
                     /locus_tag="POA77_001762"
     CDS             11179..11664
                     /gene="rraA"
                     /locus_tag="POA77_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDI6365209.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(11749..11994)
                     /gene="zapB"
                     /locus_tag="POA77_001763"
     CDS             complement(11749..11994)
                     /gene="zapB"
                     /locus_tag="POA77_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDI6365210.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            12419..13264
                     /gene="glpF"
                     /locus_tag="POA77_001764"
     CDS             12419..13264
                     /gene="glpF"
                     /locus_tag="POA77_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDI6365211.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            13287..14795
                     /gene="glpK"
                     /locus_tag="POA77_001765"
     CDS             13287..14795
                     /gene="glpK"
                     /locus_tag="POA77_001765"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI6365212.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            14931..15941
                     /gene="glpX"
                     /locus_tag="POA77_001766"
     CDS             14931..15941
                     /gene="glpX"
                     /locus_tag="POA77_001766"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI6365213.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            16038..16784
                     /gene="fpr"
                     /locus_tag="POA77_001767"
     CDS             16038..16784
                     /gene="fpr"
                     /locus_tag="POA77_001767"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDI6365214.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(16789..17217)
                     /gene="uspD"
                     /locus_tag="POA77_001768"
     CDS             complement(16789..17217)
                     /gene="uspD"
                     /locus_tag="POA77_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDI6365215.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(17244..17543)
                     /locus_tag="POA77_001769"
     CDS             complement(17244..17543)
                     /locus_tag="POA77_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDI6365216.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(17755..18195)
                     /locus_tag="POA77_001770"
     CDS             complement(17755..18195)
                     /locus_tag="POA77_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6365217.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            18296..18895
                     /locus_tag="POA77_001771"
     CDS             18296..18895
                     /locus_tag="POA77_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDI6365218.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            19003..19770
                     /gene="tpiA"
                     /locus_tag="POA77_001772"
     CDS             19003..19770
                     /gene="tpiA"
                     /locus_tag="POA77_001772"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI6365219.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(19825..20580)
                     /gene="cdh"
                     /locus_tag="POA77_001773"
     CDS             complement(19825..20580)
                     /gene="cdh"
                     /locus_tag="POA77_001773"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDI6365220.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(20687..21676)
                     /gene="sbp"
                     /locus_tag="POA77_001774"
     CDS             complement(20687..21676)
                     /gene="sbp"
                     /locus_tag="POA77_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDI6365221.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKVQKEHFANGGTFDQISKR"
     gene            complement(21995..22957)
                     /gene="pfkA"
                     /locus_tag="POA77_001775"
     CDS             complement(21995..22957)
                     /gene="pfkA"
                     /locus_tag="POA77_001775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI6365222.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(23138..24040)
                     /gene="fieF"
                     /locus_tag="POA77_001776"
     CDS             complement(23138..24040)
                     /gene="fieF"
                     /locus_tag="POA77_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDI6365223.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(24189..24689)
                     /gene="cpxP"
                     /locus_tag="POA77_001777"
     CDS             complement(24189..24689)
                     /gene="cpxP"
                     /locus_tag="POA77_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDI6365224.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            24839..25537
                     /gene="cpxR"
                     /locus_tag="POA77_001778"
     CDS             24839..25537
                     /gene="cpxR"
                     /locus_tag="POA77_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDI6365225.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            25534..26907
                     /gene="cpxA"
                     /locus_tag="POA77_001779"
     CDS             25534..26907
                     /gene="cpxA"
                     /locus_tag="POA77_001779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDI6365226.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(27013..27687)
                     /gene="yiiM"
                     /locus_tag="POA77_001780"
     CDS             complement(27013..27687)
                     /gene="yiiM"
                     /locus_tag="POA77_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDI6365227.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(27836..28819)
                     /gene="kdgT"
                     /locus_tag="POA77_001781"
     CDS             complement(27836..28819)
                     /gene="kdgT"
                     /locus_tag="POA77_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDI6365228.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(29079..29699)
                     /gene="sodA"
                     /locus_tag="POA77_001782"
     CDS             complement(29079..29699)
                     /gene="sodA"
                     /locus_tag="POA77_001782"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDI6365229.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            29984..31018
                     /gene="rhaT"
                     /locus_tag="POA77_001783"
     CDS             29984..31018
                     /gene="rhaT"
                     /locus_tag="POA77_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDI6365230.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(31015..31881)
                     /gene="rhaR"
                     /locus_tag="POA77_001784"
     CDS             complement(31015..31881)
                     /gene="rhaR"
                     /locus_tag="POA77_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDI6365231.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(31937..32773)
                     /gene="rhaS"
                     /locus_tag="POA77_001785"
     CDS             complement(31937..32773)
                     /gene="rhaS"
                     /locus_tag="POA77_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDI6365232.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            33061..34530
                     /gene="rhaB"
                     /locus_tag="POA77_001786"
     CDS             33061..34530
                     /gene="rhaB"
                     /locus_tag="POA77_001786"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDI6365233.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            34527..35786
                     /gene="rhaA"
                     /locus_tag="POA77_001787"
     CDS             34527..35786
                     /gene="rhaA"
                     /locus_tag="POA77_001787"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDI6365234.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLSWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            35961..36785
                     /gene="rhaD"
                     /locus_tag="POA77_001788"
     CDS             35961..36785
                     /gene="rhaD"
                     /locus_tag="POA77_001788"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDI6365235.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            36795..37109
                     /gene="rhaM"
                     /locus_tag="POA77_001789"
     CDS             36795..37109
                     /gene="rhaM"
                     /locus_tag="POA77_001789"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDI6365236.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8824 a         9838 c         9786 g         8702 t
ORIGIN      
        1 tcctgtcgtt ctgtattgtg gtcagccggt cgccgtccca gccgtaccag gtcacttgcg
       61 gtttccgtga cagcgacatc cagcccgtca ggtcccgttc acgccgccat acccgttttg
      121 ccaccctgcg gcccagcggg tcgtaaagat agcgactttc gaccagcggc tcttcatatt
      181 gtgtccgcgt gtagtgcacc agccggtgct gactgtcgta atggtaccgg tgagtccgct
      241 catcatccgt gcggataacc ccttccggga tgaggtcggt tttctctgtc agcctgccgt
      301 gacggtcata ccggtaaaga tagtgcgcgt cacgggcgat acggttatcc ggccacatgc
      361 tgagggtgct gtccgggtgc agctccgggt cgggcaggcg gttgcccgcc gggtccgtgg
      421 catacgggat gcggatatcc agattcgctg cggtggtgtg aacgccggtc agcctgccgg
      481 tggtgctgta gctgtaactc cgggtctggc gcgggctgct gatgcggatg agttcgccgt
      541 tgtcgttcca ggtgtaatcg cggtcatact gcaggctgtt caggtgctga cgctgtaact
      601 gcccggcagg ggtgtaagcg gtggtgagtt cataacgacc gaagctgcgc agcgtttccc
      661 ggtgcaggcg gtcgcgggtg aaatccacca gcggtgtgtc gccgagcttc atgcctgcca
      721 ggtaaccgct gccgtaggtc agccattcca cggcgggcag gctgtccggt atacagcggt
      781 tcgccagtcc ctgcgcgttg tacgcatgtc tggtctcatg ctgccagagc agtgcttccg
      841 tctgcgggtg atgcaccgtc tggcgctcac cggtcaggcg gcctttctca tcatacctgt
      901 aatgcaccgc tacccggtgc ccttcgctga tatggctgat gtctgtcagc cagccacgtt
      961 cgtcataccg ccaccgctct gcggtttcgc cattcacggt gcggtgcgtg aggcggtctg
     1021 cttcgtcata gtgccagtgg gtgaccagcc cctcatcctc gctgcggata agtttgccgg
     1081 tcaggtcgtg gtgataacgc tgtgtgcggc cgtcaaagcc ggtttcctgt atcagccggt
     1141 cgagtacatc gtaacggaag gtggtgtggc tgccgttttc actggtcagg cggatgaccc
     1201 gtccggcagc atcgtattcc atactgcgcg tcagaccgcc ctgcgtggta cagatggctt
     1261 ttccccacgc atcgtactgt gtcccgtttc tgctgccgtc cggggcaatg acggtggtca
     1321 ggtcaccggc ggcgttgtat tcataccgcg tttcatggcc ctgcgtgtct ttcacggcaa
     1381 ttaactgtcc acggctgtcg tatgcgcggt actgactcag cccttcctcg cggtgcaccg
     1441 ccgtcacctg accaaaacgg tcatggtcat agcgggttac ataaccggaa cagtcagtga
     1501 agctcagcaa ctgaccgtaa cggctccacg tcatggtttt ccggctgccg gtggcatcgt
     1561 ccgttgcgca ggggaggtca ctgtgtggat tatcataacg gtagcgggtg atatcgccgt
     1621 caggggcggt ttcctgaatc agacgtccgt actcatcata tttgcggcgc atttccagcc
     1681 cgtctggccc ggtggctgac gttaactggc tgtggtggtt atagtaaaac gccgatgccc
     1741 tgccatccgg cgtggtgatg cgcgtgatga ggcccgtcac cacatccggg ctgtactctg
     1801 ttgtccggcc tgcggcatcc gtctgtgcct taagcctgcc caccgcatca aactgactct
     1861 gcgtgacgct gccgtccgcg tgttcctttt tcaccacccg cttcagcccg gcttcgccct
     1921 gcgtgtgcag cacttcacgg cggtccaggc tgtcggtgat ggtgatgtgg tctttctcat
     1981 actgatacgt gtagcttaag cctgccgggt taagctgttc tgtcacccgc ccgtcgctgt
     2041 cgtaacggta acggatttcc ggccggcccg tgtgacggtg cgccaccatc cggccccggt
     2101 atttatcatc gtaagtaaag ctgcgcacct gtttgccact acggtcatac accaccgcca
     2161 gttcgccgcg tggcgtccag ccatagcgca ccagcggcgc ggcaggtaaa ttctccgggt
     2221 attccgggtc gtgcgtcagc cacacggcag acagacggat gccgttgtcc cggccatatt
     2281 cggtgtaacc cggcagggta tcaggaaaag cggacggttc cgtcccgccg gaaatggcct
     2341 gctgccgggc ttcttctgcc cgctgcgcct gcgtggtcag taccagccgg aagtgacgcc
     2401 cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc accggcggct tcgcggtgga
     2461 acgtctgtgt gcgcccgaag cggtccacca gcccggtcag tacccggtac ggcggcagcg
     2521 gcgcaggcag cacctcatcc gcttccggca cccgctcaca ccagccgagc agccaccacg
     2581 gcccctgcgg actgtttgtc gccagataac gatgcggact taagcggagt tcttccggca
     2641 gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccacg ccgccgcgca
     2701 ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg tgctcaaaat
     2761 acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc tgtaagcgga
     2821 tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc gtttttgtcc
     2881 ggtaactgct gtaggtgcgg gagaggatga acggcagcgg gccgggcagg gcgatgtcgg
     2941 tttcaccggg aaggaccttt gcaccgagca ggggattgac cggatggccg gacgtcactc
     3001 cgccggggca caccgaacag gccacgccgg tgggggcacc gatgcgcacc ccggctgaac
     3061 cctggacaat gctaccgcca tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc
     3121 tcatcgtaaa atccttatga aataaataag aaccagaagc ctagcgccag tcaacaacag
     3181 gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc aaattacagg
     3241 cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg ctgcccggga
     3301 tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca cgctgtttgc
     3361 cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg
     3421 agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg tatttcgggt
     3481 gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc cagccctaac
     3541 gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt acgaaggcgc
     3601 gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct acggcaatgt
     3661 gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc cacgttgcct
     3721 tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg acggttaaag
     3781 ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg gttgtggtgt
     3841 cagttagcga tgtcagtgag ctgccgctta acgagctgaa agcggtagtg gaagtgctgg
     3901 acgttgagcc ggtatttact cactccgtct ggcggttact gctctgggcg gcagattact
     3961 atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg cgccaggggc
     4021 ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggc caggcggtgg
     4081 atctgaacag cctgaaacgc tcccccaagc aacaacaggc gctggcggcg ttacggcaag
     4141 gcaaaatctg gcgcgaccag gtcgccacgc tcgaatttaa tgatgccgcg ttgcaggcgc
     4201 tacgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc gactggcaaa
     4261 cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc accgccgttg
     4321 gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc gttaccggtt
     4381 ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg
     4441 cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt tttcgtgaac
     4501 gttttaatgc ccccgtggaa gttctgcatt ccggcctgaa cgacagcgag cggctttcgg
     4561 cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt
     4621 ttacgccgtt taaaaatctc ggcgtgattg tcattgatga agagcacgac agctcctaca
     4681 agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg
     4741 agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc
     4801 agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa
     4861 ttcaacacgt gctggattta aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa
     4921 tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt cttaatcgtc
     4981 ggggttttgc gcctgcgcta ctgtgccacg actgcggctg gattgccgaa tgcccacgtt
     5041 gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac cactgtgaca
     5101 gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg gtacccgtgg
     5161 ggctgggcac cgaacagctt gaacagacgc ttgcgccgct gttccctgat gtgcccattt
     5221 ctcgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa ctggcggaag
     5281 tacatcgcgg cggtgcgagg attttgatcg gcacacaaat gctggcgaaa ggtcaccact
     5341 ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt tctgccgatt
     5401 ttcgctcggc agagcgtttc gctcagcttt acacccaggt cgctggtcgt gccgggcgtg
     5461 cgggtaaaca aggcgaagtg gtgctgcaaa cgcaccatcc agaacatcct ctgttgcaaa
     5521 cgttgctcta taaaggctac gacgcctttg ccgaacaggc gctggctgag cgacggatga
     5581 tgcaactgcc gccatggacc agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc
     5641 atgcgccgct gttcctgcaa caactgcgta atctgatcct ctccagcccg ctggcagacg
     5701 acaaactgtg ggttctcggt ccggttccgg ctctggcacc taagcgtggt ggccgctggc
     5761 gctggcagat cttgttgcag cacccttccc gcgtgcgctt gcaacacatc attagtggta
     5821 cgctggcgct tattaataca ataccggatt cccgcaaggt gaaatgggtg ttggatgtcg
     5881 atcccatcga aggctaatgc gcaaacgttg cgaggcggat cgaaaaattc aatattcatc
     5941 acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga
     6001 tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc
     6061 gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac
     6121 agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca cccgtaatcg
     6181 ggttgaaaaa gcggcccggg aagtgggtta tttaccgcaa cctatggggc gcaacgtcaa
     6241 gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc ccttctttag
     6301 cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg tgctgattgg
     6361 cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca tcaccaagca
     6421 aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca ttgaggaaca
     6481 gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca ccggaactgg agctacctac
     6541 agttcatatc gacaatctga ccgccgcatt tgatgcagta aattatctat atgagcaagg
     6601 gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc actaccgcct
     6661 gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc aatacatcgc
     6721 ccgtggcgac ttcaccttcg aagccggaag caaagcgatg cagcagctgc ttgatcttcc
     6781 acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg cactttctca
     6841 ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt ttgataacat
     6901 cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc gttacgaaat
     6961 cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg ttggcagtgg
     7021 ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt taccttaaag
     7081 taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac atggcgggct
     7141 gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta cgccgcagcc
     7201 aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga aatctgcctg
     7261 cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt atcggtggtc
     7321 tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc cagaaagtga
     7381 ccggtaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa gagctggaaa
     7441 gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc
     7501 cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc
     7561 agcagccaac gcagctggtt gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc
     7621 aaacgctaca acgccagcgt caggcgcagc aattagcgga gcagcaacgc ctggctcagc
     7681 agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcacaa gccgcgccgg
     7741 tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac caggatctgc
     7801 tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg ccggttgctc
     7861 gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc
     7921 agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag ctggcgttcg
     7981 aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc attggcccgg
     8041 tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa
     8101 actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat ctataattgc
     8161 attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa ggggtcagct
     8221 cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca
     8281 ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa
     8341 cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc tgttcgaact
     8401 gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg ttgagctggc
     8461 gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga
     8521 tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct
     8581 tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgtgcac tgttagaaaa
     8641 tactgaactg agtgctcgtg aaattgctga aaaggcgttg gatattgcag gcgacatttg
     8701 catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt aaggatctcc
     8761 catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca tcatcggcca
     8821 ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct
     8881 caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg
     8941 tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa
     9001 agttgaagcg accaaattca ccgaagtggg ctacgtcggt aaggaagtgg attctattat
     9061 tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg
     9121 ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc cacctgctaa
     9181 aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc aggcattccg
     9241 caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc ttgccgcagc
     9301 accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca gccagctgca
     9361 gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga
     9421 cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa
     9481 gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa
     9541 aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga
     9601 cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga
     9661 ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc
     9721 ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag gcgctgacca ccagcgactt
     9781 cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag cactgatggc
     9841 gactgaaggc gtaaatatcg agtttaccga ctccggtatt aagcgcatcg cggaagcggc
     9901 atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga
     9961 gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa ctatcatcat
    10021 tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag atctgagccg
    10081 ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct gaaaggcccc
    10141 atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg cgtggctgga
    10201 aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg ggacagcgct
    10261 ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactga ttaccgccgg
    10321 gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa aaggcagcga
    10381 taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga
    10441 gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggt tggcactggt
    10501 tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg gcgggttgtc
    10561 gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata tcggtctggg
    10621 tgatatttcc gtactggttt tctttggctg gttgagtgtc atggggagct ggtatttaca
    10681 ggctcataca ttgattccgg cactgatcct tccggcgacc gcatgcggcc tgctggcaac
    10741 ggcagtactg aatattaata acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa
    10801 cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat
    10861 gggctcactg gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg
    10921 gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga tgcgggaaat
    10981 ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt tactgactaa
    11041 cctgctgttt attttaggga tattcctaag ccagtgggca gcataactga caaatatcaa
    11101 ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa attctcgcag
    11161 caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat ctatcaagaa
    11221 gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc gtttggcgga
    11281 caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct gctcgaacag
    11341 aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc
    11401 gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt catttacggc
    11461 gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc gatggcggca
    11521 attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc
    11581 ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat tattctttca
    11641 gaagatccgc tggatattga atgatagaaa aggcaccgca aggtgccttt tttctgcgtt
    11701 acctgttggc ctacacagta aagaaattac gcgaaagatg aagcgtaatc agacctcttc
    11761 catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg
    11821 gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacag
    11881 tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat
    11941 cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct
    12001 cctgaattgc aaggcgttga tggataaaaa tcctcgtccc gattaccggt gacgccttaa
    12061 taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc gctgtccagc
    12121 ggcacgcctt gcagattacg gtttgccaca cttttcatcc ttctcctggt gacataatcc
    12181 acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac atgcatcatg
    12241 tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacgaagc acacacattt
    12301 taagttcgat atttctcgtt tttgctcgtt aacgataagt ttacagcatg cctacaagca
    12361 tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg atccgattat
    12421 gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta ccgggttgtt
    12481 gattttcttc ggtgtgggtt gcgttgcagc actaaaagtc gctggtgcgt cttttggtca
    12541 gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc tgaccgcagg
    12601 ggtttccggc gcgcatctta atcccgctgt taccattgca ttgtggctgt ttgcctgttt
    12661 cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt tctgtgctgc
    12721 ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga ctcatcacat
    12781 tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt accctaatcc
    12841 tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta ttctgatggg
    12901 gctgatcctg gcgttaacgg acgatggcaa cggtgtacca cgcggccctt tggctccctt
    12961 gctgattggt ctactgattg cggtcattgg cgcatctatg ggcccattga caggttttgc
    13021 catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tggctggcgg gctggggcaa
    13081 tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt tcggccctat
    13141 cgttggcgcg attgtaggtg catttgccta ccgcaaactg attggtcgcc atttgccttg
    13201 cgatatctgt gttgtggaag aaaaggaaac cacaactcct tcagaacaaa aagcttcgct
    13261 gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt gcgctcgacc
    13321 agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc attagcgtgt
    13381 cgcagcgcga atttgagcaa atctacccaa aaccaggttg ggtagaacac gacccaatgg
    13441 aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc gatatcagtt
    13501 ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt gtctgggaaa
    13561 aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt accgcagaaa
    13621 tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat accggtctgg
    13681 tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat gtggaaggct
    13741 ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg tggcttatct
    13801 ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct cgtaccatgt
    13861 tgttcaacat ccataccctg gactgggacg acaaaatgct ggaagtgctg gatattccgc
    13921 gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact aacattggcg
    13981 gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag gccgcgctgt
    14041 ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact ggctgcttta
    14101 tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg accaccatcg
    14161 cctgcggccc gactggcgaa gtgaactatg cgttggaagg tgcggtgttt atggcaggcg
    14221 catccattca gtggctgcgc gatgagatga agttgattaa cgacgcctac gattccgaat
    14281 atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca tttaccgggc
    14341 tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat tttcggtctg actcgtgggg
    14401 tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag acgcgtgacg
    14461 tgctggaagc gatgcaggcc gactctggta tccgtctgca cgccctgcgc gtggatggtg
    14521 gcgcagtagc aaacaatttc ctgatgcagt tccagtccga tattctcggc acccgcgttg
    14581 agcgcccgga agttcgcgaa gtgactgcat tgggtgcggc ctatcttgct ggtctggcgg
    14641 tcggtttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgaa cgcgagttcc
    14701 gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa aaagcggtca
    14761 aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtaa atgccggatg aagcgtttat
    14821 gccgcatccg gtagtcctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    14881 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    14941 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    15001 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    15061 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    15121 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    15181 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    15241 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    15301 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    15361 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctagcta
    15421 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    15481 ttccggatgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    15541 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    15601 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    15661 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    15721 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    15781 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    15841 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    15901 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    15961 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    16021 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    16081 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    16141 aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    16201 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    16261 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    16321 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    16381 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    16441 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    16501 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    16561 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    16621 aaagcgcgat tggtctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    16681 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    16741 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    16801 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    16861 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    16921 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    16981 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    17041 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    17101 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    17161 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    17221 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    17281 atctcggcct gacatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    17341 ttaatcttgt aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct
    17401 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    17461 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    17521 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    17581 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    17641 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    17701 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt
    17761 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    17821 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    17881 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgcccagt tacccgccag
    17941 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    18001 gcgcttaaca gttactgccg ctgttggcca aagcaagcac acaaggcaaa acgccgcggt
    18061 ctgaatatcg agtaaatttt tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    18121 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    18181 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    18241 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    18301 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    18361 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    18421 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccta gcctgccact
    18481 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    18541 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    18601 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    18661 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    18721 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    18781 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    18841 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    18901 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    18961 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    19021 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    19081 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    19141 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    19201 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    19261 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    19321 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    19381 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    19441 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac
    19501 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    19561 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    19621 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    19681 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    19741 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    19801 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    19861 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    19921 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    19981 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    20041 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    20101 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    20161 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    20221 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    20281 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    20341 tggcatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    20401 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    20461 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    20521 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    20581 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    20641 acccggtcac gagaaaccac cgggtttgat aattatcctg gggaaatcag cgtttgctga
    20701 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cactttcgtc cagccgccga
    20761 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcca
    20821 cctcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    20881 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    20941 cgaccaccga cacggttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    21001 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaagcg atcagtacat
    21061 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    21121 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    21181 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    21241 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    21301 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    21361 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    21421 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    21481 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    21541 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    21601 taagaagctg aatatccttt gccataacgt tggttgccgc cagcaaaaat gttaacccta
    21661 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    21721 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    21781 gggggttaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    21841 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    21901 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    21961 tcagggtaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    22021 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    22081 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    22141 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    22201 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    22261 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    22321 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    22381 gaaccacaac gaattcacag ccaccggcaa tggccgcagc caacgtcaga tcgccacaat
    22441 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    22501 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    22561 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    22621 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgatcccac
    22681 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaagc
    22741 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agaaacgctg taacggtcta
    22801 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc attacttcca
    22861 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    22921 caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa ctttggaatg
    22981 caaaataaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    23041 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    23101 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    23161 cctccctggg tacgacggaa caggggtcct gatggataat tacatccgat cccggaaaac
    23221 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    23281 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    23341 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    23401 cctcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    23461 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    23521 cgtaccagga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    23581 aatgtagcat atcagcccgc accgcctggc tttgcgtccg cctcaccacc caacgctgaa
    23641 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    23701 gatctgtcat cggtgttgga gatatcagat gttgaatacc cgtcaaaaac aggaatagtg
    23761 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    23821 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccaat aaattcgtca
    23881 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    23941 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    24001 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    24061 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    24121 taaaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    24181 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    24241 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    24301 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    24361 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    24421 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    24481 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    24541 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    24601 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    24661 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    24721 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    24781 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    24841 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    24901 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    24961 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    25021 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    25081 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    25141 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    25201 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    25261 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggcactga
    25321 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    25381 aagccaggaa gtgctgggca aacgcctgac gcctttcgac cgcgctatcg atatgcacat
    25441 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    25501 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    25561 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    25621 ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt
    25681 gagcagcatg ttgaagcgga actggcgaac gatccgccca acgatttaat gtggtggcga
    25741 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    25801 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    25861 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    25921 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    25981 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    26041 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    26101 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    26161 gaacttgaag cggggccaca ggaattcctt gccgctggtg ccagttttaa ccagatggtc
    26221 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    26281 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagtggt
    26341 gaaagcaagg aactggagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    26401 gatctgttgg tgatgtcccg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    26461 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcagatgggc
    26521 aagtcgttga cggttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    26581 ctggaaagtg cgctggaaaa tattgttcgt aatgctctgc gttattccca tacgaagatt
    26641 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    26701 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    26761 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    26821 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    26881 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    26941 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    27001 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    27061 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    27121 gtgatattga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    27181 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    27241 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    27301 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    27361 tgcctctccc cagcggaaaa tatcgcccat ataaacatta ctttccgtca ggccgtcggt
    27421 tgagaggttt tcaccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    27481 cgcccagtag agataatgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    27541 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    27601 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    27661 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    27721 accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca
    27781 atagcaaacc ggacgaacgt tagttcatcc ggctgtggag attaaggaag caggtttatt
    27841 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    27901 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    27961 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    28021 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    28081 atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga
    28141 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    28201 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    28261 ggttccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    28321 caaacgaggc aatccctgca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    28381 acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag
    28441 cgtaaagtcc gccattggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    28501 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    28561 cccacgcgac ggcaattttt gttaccacca gcgtaccgga tttacgcaat accgttcccg
    28621 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    28681 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    28741 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    28801 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtca
    28861 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    28921 agtgatcaaa accacatcaa ttgaaacact gttttatttt tatcggatca ttgtttttat
    28981 tttgaaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    29041 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    29101 gccgctgctt cgtcccagtt caccacgttc cagaactctt taatgtagtc cggacggcgg
    29161 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    29221 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    29281 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    29341 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    29401 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    29461 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    29521 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    29581 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    29641 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    29701 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggttcatta
    29761 tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt
    29821 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    29881 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    29941 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    30001 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    30061 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    30121 gattattctg ccgtgggcca tcagcgccct gttactaccg aatttctggg cgtattacag
    30181 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    30241 taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    30301 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    30361 tgtgctgatt aacaccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat
    30421 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaaaatgg gcattaaagc
    30481 cgaagagttc aacctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    30541 cggtatgtct tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcactggg
    30601 cgtcgatcca ctgtatgtcg ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat
    30661 catcaacctc ggtttctgct tcattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    30721 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcag cactgggcgg
    30781 gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca
    30841 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    30901 cgggctggtg ctgaaagagt ggaacaatgc aggtcgccgt ccggtaacgg tgttgagcct
    30961 cggttgtgtg gtgattattg tcgccgccaa catcgtcggc atcggcatgg cgaattaatc
    31021 tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac
    31081 caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag
    31141 gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca gatattgatt
    31201 gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc
    31261 ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat
    31321 cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg
    31381 gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt tagcaaacga
    31441 cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc
    31501 cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc
    31561 ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag
    31621 atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg
    31681 ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac
    31741 cagctcacaa aaatcatgtg tatgttcggc aaaaacatct tgcggataac ggtcagccac
    31801 agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc
    31861 caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac
    31921 gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata tcacgcggtg
    31981 accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc
    32041 gataggcgat atcagtaacg ctggcctcgc tgtggcgtag tagatgtcgg gctttcatca
    32101 gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta agctgccgat
    32161 gtagcgtacg cagcgaaaga gaaaattgct ccgccacggc atcccaattc acctcatcgg
    32221 caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt
    32281 tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac
    32341 tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc
    32401 gcacctgctg caatacgctg tggttaacgc gccagtggga aggatactgc ccatccagct
    32461 cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca
    32521 gcacattagt cagacacaga ttatcggtat gttcatacag atgccgatca tggtcgcgca
    32581 caaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg
    32641 tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct
    32701 gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat
    32761 gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt
    32821 caggttctta ccttaaattt tcgacgggaa accacgtaaa aaacgtcgat ttttcaagat
    32881 acagcatgaa ttttcaggaa atgcggtgag catcacacca ccacaattca gcaatttgtg
    32941 aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt cagtagcgag
    33001 aaggtcgcga attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt
    33061 atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg
    33121 gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaaaaat
    33181 gggctgcata gccagaacgg ttatgtcacc tggaatgtgg atagcctgga aagtgccatt
    33241 cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggattgat
    33301 acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt
    33361 gcttatcgcg atagccgctc caatggccta atggcgcagg cacagcaaca actcggcaaa
    33421 cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcaactg
    33481 cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctgatg
    33541 ccggattact tcagctatcg cctgaccggc aagatgaact gggagtacac caatgccacc
    33601 accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc
    33661 ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg
    33721 atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc
    33781 gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc tggcacctgg
    33841 tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaac
    33901 atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta
    33961 tggctgcttc agcgagtgct tcaggaacgg caaatcaacg atctcccggc gcttatcgcc
    34021 gcgacacagg cacttccggc ctgtcgcttc atcatcaatc ccaatgacga tcgctttatt
    34081 aatcctgaag cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc
    34141 ccggaaagtg atgctgaact ggcgcgctgt attttcgaca gtctggcgct gctgtatgcc
    34201 gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt tctcgcaact gcatattgtc
    34261 ggcggcggct gccagaacac gctgctcaac cagctatgcg ccgatgcctg cggtattcgg
    34321 gtgatcgccg ggcctgttga agcctcaacg ctcggcaata tcggcatcca gttaatgacg
    34381 ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac cgcgaatctg
    34441 accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca gattcactct
    34501 acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg
    34561 cgaaacagcg tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc caacttgatc
    34621 gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg
    34681 aaggttcgct gaccggtggg attcaagcta ctggtaatta tccgggcaaa gcgcgtaatg
    34741 ccagtgagct acgtaccgat ctggaacagg ctatgcggct gattccgggg ccgaaacggc
    34801 ttaatttaca tgccatctat ctggaatcag atacgccagt ctcgcgcgat cagatcaaac
    34861 cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta
    34921 acccctcctg cttttcgcat ccgctaagcg ccgatggctt tacgctttcc catgccgacg
    34981 acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt tcggcctatt
    35041 ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat ggtatgaaag
    35101 atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg gatgaggtga
    35161 tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca
    35221 ttggcgcaga gagctacacg gttggctcca atgagtttta catggggtat gccaccagcc
    35281 gccagactgc gctgtgcctg gacgccgggc acttccaccc gactgaagtg atttccgaca
    35341 agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagc cgtccggttc
    35401 gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt gccagtgaga
    35461 ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct tgacttcttc gatgcctcta
    35521 tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc ctgctgcgtg
    35581 cgttgctgga acctaccgcc gagctgcgca agttggaagc ggcgggcgat tacactgcgc
    35641 gtctggcact gctggaagag cagaaatcgt tgtcgtggca ggcggtctgg gaaatgtatt
    35701 gccaacgtca cgatacccca acgggtagcg aatggctgga gagcgtgcgg gcttatgaga
    35761 aagcgatttt gagccagcgt gggtaaacac tgccggatgc ggcgcgagcg ccttatccgg
    35821 cctacaggtc ggcaacagtt gtaggcctga taagacgcga cagcgtcgca tcaggcattg
    35881 attgccggat gcggcacgag cgccttatcc ggcctacggg tcgtgcatct gacaacaccg
    35941 aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga
    36001 tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaacggc ggcaacctga
    36061 cgctgcgcct ggatgacgcc gatatcgcgc cttataaaga caacttccat gcacaaccgc
    36121 gctatatccc gctcagccag cccatgcctt tactggcaaa tacaccgttt attgtcaccg
    36181 gctccggcaa attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa
    36241 aagtcgacag cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc
    36301 caacttctga acttccggct cactttctct cccactgcga gcgcattaaa gccaccaacg
    36361 gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac
    36421 tggaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc gagtgtctgg
    36481 tggtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg
    36541 gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc ttccacggcg
    36601 tcttcggcag cgggtcgacg ttggatgaaa cattcggttt aatcgacacc gcagaaaaat
    36661 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg
    36721 aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagccagt gcgctggcgc
    36781 tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg
    36841 aagagtatca gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc
    36901 acggtgcgca taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg
    36961 tagagattga atctgaagag cgctggaatg cggttgccag tactgatgtt tgccaacgtt
    37021 ggtggaaata tatgaccgac gtcatgcccg cgaacccgga taacagcccg gtgagtagcg
    37081 aactgcaaga agtgttttac ctgccgtaat tgcctaatgt gccggagacg cctccggcac
    37141 aatattttta
//
LOCUS       DAMZII010000037        36841 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZII010000037 DAMZII010000000
VERSION     DAMZII010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.037"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            80..1219
                     /gene="arnB"
                     /locus_tag="POA77_001790"
     CDS             80..1219
                     /gene="arnB"
                     /locus_tag="POA77_001790"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDI6365237.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1223..2191
                     /gene="arnC"
                     /locus_tag="POA77_001791"
     CDS             1223..2191
                     /gene="arnC"
                     /locus_tag="POA77_001791"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDI6365238.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2191..4173
                     /gene="arnA"
                     /locus_tag="POA77_001792"
     CDS             2191..4173
                     /gene="arnA"
                     /locus_tag="POA77_001792"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDI6365239.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYATDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4170..5060
                     /locus_tag="POA77_001793"
     CDS             4170..5060
                     /locus_tag="POA77_001793"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDI6365240.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5060..6712
                     /gene="arnT"
                     /locus_tag="POA77_001794"
     CDS             5060..6712
                     /gene="arnT"
                     /locus_tag="POA77_001794"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDI6365241.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6709..7044
                     /gene="arnE"
                     /locus_tag="POA77_001795"
     CDS             6709..7044
                     /gene="arnE"
                     /locus_tag="POA77_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDI6365242.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7044..7430
                     /gene="arnF"
                     /locus_tag="POA77_001796"
     CDS             7044..7430
                     /gene="arnF"
                     /locus_tag="POA77_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDI6365243.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7424..7690)
                     /gene="pmrD"
                     /locus_tag="POA77_001797"
     CDS             complement(7424..7690)
                     /gene="pmrD"
                     /locus_tag="POA77_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDI6365244.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7800..9155)
                     /gene="menE"
                     /locus_tag="POA77_001798"
     CDS             complement(7800..9155)
                     /gene="menE"
                     /locus_tag="POA77_001798"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI6365245.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9152..10114)
                     /gene="menC"
                     /locus_tag="POA77_001799"
     CDS             complement(9152..10114)
                     /gene="menC"
                     /locus_tag="POA77_001799"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI6365246.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10114..10971)
                     /gene="menB"
                     /locus_tag="POA77_001800"
     CDS             complement(10114..10971)
                     /gene="menB"
                     /locus_tag="POA77_001800"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6365247.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10986..11744)
                     /gene="menH"
                     /locus_tag="POA77_001801"
     CDS             complement(10986..11744)
                     /gene="menH"
                     /locus_tag="POA77_001801"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI6365248.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11741..13411)
                     /gene="menD"
                     /locus_tag="POA77_001802"
     CDS             complement(11741..13411)
                     /gene="menD"
                     /locus_tag="POA77_001802"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI6365249.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13500..14795)
                     /gene="menF"
                     /locus_tag="POA77_001803"
     CDS             complement(13500..14795)
                     /gene="menF"
                     /locus_tag="POA77_001803"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI6365250.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGETAFLGSSPERLWRRRDNALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14874..15179)
                     /gene="elaB"
                     /locus_tag="POA77_001804"
     CDS             complement(14874..15179)
                     /gene="elaB"
                     /locus_tag="POA77_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDI6365251.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15234..15695)
                     /locus_tag="POA77_001805"
     CDS             complement(15234..15695)
                     /locus_tag="POA77_001805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6365252.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15760..16677
                     /gene="rbn"
                     /locus_tag="POA77_001806"
     CDS             15760..16677
                     /gene="rbn"
                     /locus_tag="POA77_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDI6365253.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16865..18085
                     /gene="elaD"
                     /locus_tag="POA77_001807"
     CDS             16865..18085
                     /gene="elaD"
                     /locus_tag="POA77_001807"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDI6365254.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSEDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSEFMGEMSNNNIKVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            18497..19103
                     /locus_tag="POA77_001808"
                     /pseudo
     CDS             18497..19103
                     /locus_tag="POA77_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19206..19709
                     /locus_tag="POA77_001809"
     CDS             19206..19709
                     /locus_tag="POA77_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDI6365255.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(19776..21233)
                     /gene="nuoN"
                     /locus_tag="POA77_001810"
     CDS             complement(19776..21233)
                     /gene="nuoN"
                     /locus_tag="POA77_001810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDI6365256.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(21240..22769)
                     /gene="nuoM"
                     /locus_tag="POA77_001811"
     CDS             complement(21240..22769)
                     /gene="nuoM"
                     /locus_tag="POA77_001811"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDI6365257.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(22933..24774)
                     /gene="nuoL"
                     /locus_tag="POA77_001812"
     CDS             complement(22933..24774)
                     /gene="nuoL"
                     /locus_tag="POA77_001812"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDI6365258.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(24771..25073)
                     /gene="nuoK"
                     /locus_tag="POA77_001813"
     CDS             complement(24771..25073)
                     /gene="nuoK"
                     /locus_tag="POA77_001813"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDI6365259.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(25070..25624)
                     /gene="nuoJ"
                     /locus_tag="POA77_001814"
     CDS             complement(25070..25624)
                     /gene="nuoJ"
                     /locus_tag="POA77_001814"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDI6365260.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(25636..26178)
                     /gene="nuoI"
                     /locus_tag="POA77_001815"
     CDS             complement(25636..26178)
                     /gene="nuoI"
                     /locus_tag="POA77_001815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDI6365261.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(26193..27170)
                     /gene="nuoH"
                     /locus_tag="POA77_001816"
     CDS             complement(26193..27170)
                     /gene="nuoH"
                     /locus_tag="POA77_001816"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDI6365262.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(27167..29893)
                     /gene="nuoG"
                     /locus_tag="POA77_001817"
     CDS             complement(27167..29893)
                     /gene="nuoG"
                     /locus_tag="POA77_001817"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDI6365263.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(29946..31283)
                     /gene="nuoF"
                     /locus_tag="POA77_001818"
     CDS             complement(29946..31283)
                     /gene="nuoF"
                     /locus_tag="POA77_001818"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDI6365264.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(31280..31780)
                     /gene="nuoE"
                     /locus_tag="POA77_001819"
     CDS             complement(31280..31780)
                     /gene="nuoE"
                     /locus_tag="POA77_001819"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDI6365265.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(31783..33585)
                     /gene="nuoC"
                     /locus_tag="POA77_001820"
     CDS             complement(31783..33585)
                     /gene="nuoC"
                     /locus_tag="POA77_001820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDI6365266.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAVIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(33679..34341)
                     /gene="nuoB"
                     /locus_tag="POA77_001821"
     CDS             complement(33679..34341)
                     /gene="nuoB"
                     /locus_tag="POA77_001821"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDI6365267.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(34357..34800)
                     /gene="nuoA"
                     /locus_tag="POA77_001822"
     CDS             complement(34357..34800)
                     /gene="nuoA"
                     /locus_tag="POA77_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDI6365268.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(35431..36369)
                     /gene="lrhA"
                     /locus_tag="POA77_001823"
     CDS             complement(35431..36369)
                     /gene="lrhA"
                     /locus_tag="POA77_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDI6365269.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         9053 a         9993 c         9306 g         8489 t
ORIGIN      
        1 gctccaaatg gcaaaatata cacaacactc tttatagcaa atataagtgg acaggtattc
       61 aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt tcgcgaccag caatgggcgt
      121 ggaggaactc gctgcagtta aagaggttct cgaatccggt tggatcacaa ccggtccgaa
      181 gaatcaggcg cttgagcaag ccttttgcca gttgacggga aatcagcatg ccatcgcggt
      241 cagttcagcc accgccggaa tgcatatcac gctaatggcg ttggaaattg gcaagggcga
      301 tgaagtgatt acgccttccc tgacctgggt ttcaaccctc aatatgattt ccttgttggg
      361 tgcaacgccg gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc ctgaagcgat
      421 cgagtcagcc ataacgccac gcactaaagc catcattccg gtgcattatg ccggtgcgcc
      481 agcagatatt gacgccattc gcgccattgg cgaacgttac ggcatcgcag ttatcgaaga
      541 tgctgcccat gccgtcggta cgtattacaa agggcgacat attggcgcaa aaggtaccgc
      601 tattttttca tttcatgcca ttaaaaatat tacctgtgcg gaaggtggcc tgattgtaac
      661 tgataatgaa aaccttgccc gccagctacg gatgctgaaa tttcacggtc tgggtgtcga
      721 tgcctatgac agacaaacct ggggccgtgc accgcaggct gaagtcttaa caccgggcta
      781 taagtacaat ctgaccgata ttaacgccgc aattgccctg acacagttag caaaattaga
      841 gcacctcaac acccgtcggc gcgaaattgc ccagcaatat cagcaagcac tggcagctct
      901 cccctttcag ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc atctgtttat
      961 tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg aagcgttaaa
     1021 agaaagaggc attggtaccg ggttacattt ccgcgccgct cacacacaaa aatattatcg
     1081 cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg aatagcgaac gcatctgttc
     1141 tttgccgctg ttcccggata tgactaccgc cgatgccgac cgtgtcatca cagcccttca
     1201 gcaactcgca ggacaataag ccatgtttga aatccaccct gttaagaaag tctcggtggt
     1261 tattcccgtt tataacgagc aggaaagctt accggaatta atcaggcgca ccaccacagc
     1321 ctgtgaatcg ttggggaaag agtatgagat cctgctgatt gatgacggca gtagcgataa
     1381 ttccgcgcat atgctggtcg aagcctcaca agcggagaac agccatattg tgtctatttt
     1441 gcttaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc acgttaccgg
     1501 cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa tcccccgcct
     1561 ggtggcaaaa gccgatgaag gttacgacgt ggtagggact gtacgccaga accgccagga
     1621 cagctggttt cgtaaaacag cttcgaagat gattaaccgg cttattcagc gcaccaccgg
     1681 caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc
     1741 gatgttgcac tgccatgaac gtagcacctt tatcccgatt ctggcgaata tcttcgcccg
     1801 ccgtgccatt gaaattccag tacatcatgc cgagcgtgag tttggtgaat ccaaatacag
     1861 ttttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca caacgccgct
     1921 acgtatgctg agtctgctcg gcagcattat tgcgattgga ggttttagca ttgcggtgct
     1981 gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg tctttatgct
     2041 atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat tactcggtga
     2101 atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt
     2161 tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat
     2221 atgggatgcc tcggtattga agctctgctg gctgccggtt acgaaattag cgccattttt
     2281 acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctcg tctggcagcg
     2341 gaaagaggca ttccggttta tgcgacggat gacgttaatc atccgctgtg ggtggaacgc
     2401 attgcccaac tgtcgccaga tgtgattttc tctttttatt atcgccatct tatttgcgac
     2461 gaaattttgc agctcgctcc cgcaggtgca tttaatctgc atggttcgct gttaccaaaa
     2521 tatcgtggtc gcgcgccgct gaactgggtg ctggtcaacg gtgaaacgga aactggcgtt
     2581 acattgcacc gaatggtgaa acgtgccgat gccggggcca ttgtggcgca actgcgcatt
     2641 gccattgcgc cagacgatat cgctattacg ctgcatcata aattgtgcca tgccgcgcgc
     2701 cagctactgg aacagacatt acccgccatt aaacacggta atattctgga aatcgcccag
     2761 cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg atgacagttt ccttgaatgg
     2821 cataaaccgg catccgtact gcacaacatg gtacgtgccg ttgccgatcc gtggccgggt
     2881 gccttcagct atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt tcatcctcat
     2941 gccagcaaag cacagccggg gagcgtgatt tctgttgcgc cactgctgat tgcctgtggc
     3001 gatggcgcgc tggaaatcgt caccggacag gcgggcgacg gcattactat gcagggctcg
     3061 caattagcgc agacgctggg cctggtgcaa ggttcacgct tgaatagcca gcctgcctgc
     3121 accgcccgac gccgtacccg ggtactcatc ctcggggtga atggctttat tggcaaccat
     3181 ctgacagaac gcctgctgcg cgaagatcat tatgaagttt acggtctgga tattggcagc
     3241 gatgcgataa gccgttttct gaatcatccg cattttcact ttgttgaagg cgatatcagt
     3301 attcattccg aatggattga gtatcatgtc aaaaaatgtg atgtcgtctt gccgctggtg
     3361 gcgatagcca cgccgattga atatacccgc aacccgctgc gcgtatttga actcgatttt
     3421 gaagagaatc tgcgcattat ccgctactgc gtgaagtacc gtaagcgaat catcttcccg
     3481 tcaacttcag aagtttatgg gatgtgtagc gataaatact tcgatgagga ccattctaat
     3541 ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt cggtatcaaa acaattactt
     3601 gatcgggtga tctgggccta tggcgaaaaa gagggtttac agttcaccct cttccgcccg
     3661 tttaactgga tgggaccgcg actggataac cttaatgcgg cacgaatcgg cagctcccgc
     3721 gctattacgc aactcattct caatctggta gaaggttcac cgattaagct gattgatggc
     3781 ggaaaacaaa aacgctgctt tactgatatt cgcgatggta tcgaggcgtt ataccgcatt
     3841 atcgaaaatg cgggaaatcg ctgcgacggt gaaattatca acattggcaa tcctgagaac
     3901 gaagcgagca ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa acatccgctg
     3961 cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagcag ctactacggc
     4021 aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca ccgctgcctg
     4081 gactgggagc cgaaaattga tatgcaggaa accatcgacg aaacgctgga tttcttcctg
     4141 cgcaccgttg atcttacgga taaaccatca tgaccaaagt aggcttacgc attgatgtcg
     4201 atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg agtaagcata
     4261 atattcaggc cagcattttt ttcagcgtcg gcccggacaa tatgggccgc catctctggc
     4321 gactggtgaa gccacagttt ttgtggaaga tgctgcgctc aaatgcggca tcgctttatg
     4381 gctgggatat tttactggca ggtacggcct ggccaggtaa agagattggt catgccaatg
     4441 ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg cctgcacgcc tgggatcacc
     4501 atgcctggca agcccgtagc ggtaactggg atcggcaaac aatgatcgac gatattgcac
     4561 gcggtcttcg cactctggaa gagattatcg gtcaaccggt aacctgttct gccgctgcgg
     4621 gctggcgtgc cgaccagaag gtgatcgaag caaaagaagc gttccatttg cgctacaaca
     4681 gcgattgtcg tggggccatg ccgttccgtc cattgctcga atcaggaaac cctggcactg
     4741 cgcaaattcc ggtgacctta cccacctggg atgaagtgat tggtcgggat gtgaaagcag
     4801 aagattttaa cggttggtta ctcaaccgca tcctgcgaga taaaggcacg ccggtttata
     4861 ccattcatgc agaagttgaa ggctgcgctt atcagcataa ttttgtggat ctcctcaaac
     4921 gcgcagctca ggaaggcgtg acattttgcc ctttaagcga actgttatca gagacgttgc
     4981 cgctcggaca agttgttcgc ggaaatattg ccggacgtga aggctggctg ggttgccaac
     5041 aaattgcggg tagtcgctga tgaaatcggt acgttacctt atcggcctct tcgcgtttat
     5101 tgcctgctat tacctgttac cgatcagcac gcgtctgctc tggcaacccg atgaaacgcg
     5161 ttatgcggaa atcagtcgag aaatgctggc atccggcgac tggattgtgc cccatctgtt
     5221 agggctacgt tatttcgaaa aacccattgc cggatactgg attaacagca ttgggcaatg
     5281 gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga ccctgttaac
     5341 tgccgcgctg gtgacctggt ttactctgcg cttatggcgc gataaacgtc tggctctact
     5401 cgccacagta atttatctct cattgtttat tgtctatgcc atcggcactt atgccgtgct
     5461 cgatccgttt attgcattct ggctggtggc gggaatgtgc agcttctggc tggcaatgca
     5521 ggcacagacg tggaaaggca aaagcgcagg atttttactg ctgggaatca cctgcggcat
     5581 gggggtgatg accaaaggtt ttctcgccct tgccgtgccg gtattaagcg tgctgccatg
     5641 ggtagcgacg caaaaacgct ggaaagatct ctttatttac ggctggctgg cggttatcag
     5701 ttgcgtactg acggttctcc cttggggact ggcgatagcg cagcgggagc ctgacttctg
     5761 gcactatttt ttctgggttg agcatattca acgctttgca ctggatgatg cccaacatag
     5821 agctccgttc tggtactacg tgccggtcat cattgccggt agcctgccgt ggctgggatt
     5881 actccccggt gcactgtaca caggctggaa aaaccgcaag cattccgcaa ccgtctattt
     5941 gttgagctgg acgataatgc cgctgctgtt tttctccgtc gctaaaggta aattgcccac
     6001 ctatattctt tcctgctttg catctctggc aatgctgatg gcgcattacg ctttgctggc
     6061 agcaaaaaat aatcctctgg cgctgcggat taatggctgg attaacatcg cttttggcgt
     6121 cactggcatt attgccacat ttgtggtctc cccgtgggga ccaatgaaca cgccggtgtg
     6181 gcaaaccttc gagagctata aagtcttttg tgcctggtcg attttttcgc tatgggcatt
     6241 tttcggctgg tacaccttaa caaacgtcga aaagacctgg ccttttgccg cgctttgccc
     6301 gctggggctg gcgttgctgg taggattttc aattcctgac agagttatgg aaggaaaaca
     6361 tccgcaattt tttgtcgaga tgacacaaga atcacttcag ccaagccgct atattcttac
     6421 tgatagcgtc ggtgttgccg caggtctggc atggagcctg caacgcgatg acatcatcat
     6481 gtatcgccag acaggtgagt tgaaatacgg ccttaattat ccggatgcga aagggagatt
     6541 tgtcagcggt gatgagttcg caaactggct taatcaacat cgtcaggagg ggattattac
     6601 tctcgtgctt tcggttgacc gcgatgaaga tatcaacagt ctcgccattc cgcccgcaga
     6661 tgccatcgat cgtcaggagc gtctggtgct gattcagtat cgtcccaaat gatctggcta
     6721 acattagtct ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa acaggcaacc
     6781 tgctttgtgg cgataaacaa acggcgcaaa catatcgtgc tgtggctggg actggcgctg
     6841 gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct tgcagaacgt accggtaggc
     6901 attgcctacc cgatgttaag tctgaatttt gtctgggtga cgctggctgc agtaaaactg
     6961 tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat tggtggcatt
     7021 gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg attattcagc gtcattattg
     7081 cctcagttgc gcaattaagc ctgggttttg cggcaagtca tctgccgccg atgacgcact
     7141 tatgggattt tatcgcagct ctgttagctt ttggcctgga tgcccggata ctgttactcg
     7201 ggctgctggg ttatctgctt tccgtttttt gttggtataa aacgctacat aaacttgcgc
     7261 taagcaaagc ctatgccttg ctcagtatga gttatgtgct ggtatggatt gcttcaatgg
     7321 tacttcccgg ctgggaaggg actttttcgc tgaaagcact actgggagta gcctgtatta
     7381 tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca acgttactga gttttccctg
     7441 ccgctgtaca ctgccgttcc cactcatcgg ggctataact gcttgccgag aggactttca
     7501 ccgtatgcag cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc gagagtaaat
     7561 cccccacttt cacggcgaag tcactcttca cttcggcgat catttttatc gcgccgccag
     7621 catcgcagag cataagtaca tgtctgttat cttgtttatt gcagcacgat tttttgacca
     7681 gccattccat tgcattatcc tgtttgctaa gagttttcac atcaatttac agattaacag
     7741 ataaactctt tatcaacggc aatggaatgg cagaaatgag aagtgaaacg gagggttaat
     7801 tattgctgac gttgcaccca ctcttttagc gcctgacgtg aaattttaat accgccgttt
     7861 ttcagctccg gcggtaaagt cagccagcgc accggttgtt gaaaacgagc cagcttatct
     7921 ttcacccatt cgccaagatc aacggtttgc tggtcatact ccaccaccgc taccggccga
     7981 tgaccaaact ccttgtcggc aacgggaacg ataaacacct gcaaaaccgc aggatgtgca
     8041 gcaataacac gctcgacttc ttccggctga ataccctcac cgccactgaa gaacagattg
     8101 tctaaacgtc cgacaatggt cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg
     8161 taccagcctt cgtcattaac cagtgaaacc agttgcccgt tacgccagta accttctgcc
     8221 atactggcag cccgcagcca cacttcatta ttaacgattt tcacttcccg acccggcagc
     8281 ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac acaccgtgga ggcaaactcg
     8341 gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg
     8401 accgggatag ccgcgccgcc aagtaacacc gctttcaggg aaacggaact gcggttaacc
     8461 agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag tacagcctgc cagcatttgc
     8521 tccaatggct gtttatcacg caccgtcatc cgcgcaccag cgtataacca gcgccacata
     8581 attccctgac cggagacgtg aaacagcggt aaagagagca accagtcatc gtgatcgcca
     8641 aacggaatca acgataacac cccttcggca ctggcaagat gggcctgata agtatggaca
     8701 gcggctttcg gcaacccggt agaacccgag gtcaacgtca ttgagcacag acgcgtcggc
     8761 tgccacgtag cggcatgtgc gccttcaacc cgctgaatgt gcagcgacgt taacgccgga
     8821 aacgtgtttt ccccatccgg caccagcgca aattgcagcg tcagattggg cagcaattct
     8881 tcaagcaacg gttgcggcag ctgagggttc acgggcaaca cccgcgcccc acattgcagt
     8941 aacgccagcc aggcgagcag cgtttgcggc gtattccacg cccgcaacat cacgccgctg
     9001 ccctcaacaa cgccctgcgc cgcaaagcct gaggctaatt catcgacgcg agcacaaagc
     9061 tcgcgccagt taagttgctc gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc
     9121 cgccagtgac gccacggcca gtcagagaag atcataacaa ccgctccagt gcatcaactt
     9181 ccacgacagg cagcgtgcta cccggccagc gacgtacctg ctgcgcctgc atcagatcca
     9241 gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc ggcaatccgc gccagttgcg
     9301 ttaagcctaa gctcgattca atggaagaac tgatcaccgc cgtcagcccc agcgcgtgcg
     9361 ccgcctgtac ctgctcgcgt actttctcca gactgcccgt gagcgtgggt ttgataacta
     9421 ccgcgcgcac gccctcttca gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc
     9481 aggcaatggc aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg
     9541 gctcttcgag aaacgcgatg cggtggcgat aatccgggtt aacatatttg gcaaactgct
     9601 ggcctttcag cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga tccggaattg
     9661 cctccagcaa cagattaacc accatgccgt cgcgcacggc ttcgtacaat cccactttga
     9721 ccttcgccac tttctcgcct ggcatatcag caagtttgag gatcagatcg tcaggatcgc
     9781 cattacacag tggtgccgca cggtaattgg ccgcctgcgg gagcgtatct gccagctccg
     9841 ccaatgcaca gcttacgcca aaggcaacgg aaggcatctg cggtagctcg caatcgcctg
     9901 ccagccagtt atttacccag gcaagcagca cgctttgcgc ctcttcccag gtttcctggc
     9961 tgaagcccgg cagtggggag atctcccccc acccttcgcg ctcgccctca cgcaggcaaa
    10021 cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg
    10081 ggatctgcca gcggtatacc tgcgcgctac gcattacgga ttccgtttaa atttgctgaa
    10141 atcaggctga cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta
    10201 gaacagcatg gtggcgttgc ccgccagctc ttgcagcccc gcctgcccgt cacagtcggc
    10261 gttcagcgca gctttcaggc agcgcagcgc catcgggctg ttttgcagca tttcgcggca
    10321 ccaacggacg gtttcttttt ccagatccgc cagcggtacc acggtgttga caaggcccat
    10381 atccagcgcc tgttttgcgt cgtactgacg gcacaggaac cagatttctc gcgctttttt
    10441 ctgcccgacg atacgagcca tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt
    10501 cgggccagtc tgaccgaaga tggcattatc tgctgcgata gtcaggtcgc acatcatgtg
    10561 cagaacgtga ccaccgccga tggagtagcc agccaccatc gcgacaaccg gtttcggaca
    10621 ggtacggatc tgacgctgga agtccagcac gttcaagtga tgtacgccgg aatcatcttt
    10681 atagccgccg taatcaccac gcactttctg gtcaccaccg gagcagaacg ctttatcgcc
    10741 tgcgccagtc agaatgatca cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg
    10801 gatcatctct tttaccgtca gaggacggaa ggcattgcgc acctgcggac gattaatggt
    10861 gatttttgcg ataccgtcgg tggatttttc ataacgaatg tcctcgaaac cttcggagca
    10921 gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca ggataaatca tattgtgtcc
    10981 tttagtcaga aacgcaagat ctgcgccaga cttgcgatta cgccagcggg attttcccga
    11041 tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg cagccagttc cgccgccagg
    11101 gcgcggaatt tgctgtcacg ttcaccacat aaataataaa acgcaaatgt gcgggcacta
    11161 aggttagcac gtaaatcagg ctggacggcg agagaagtcg cctccagcat ggcggcaagg
    11221 gttgcgccat tattgttgct gcgcagcgcc accagctccc ggcgttgatc gtcattgagc
    11281 gaggcaaaaa caggctgttg ataccagtcg gcaaataccg ccgttaacgg ttctgtgcaa
    11341 aaacgctgcg cccattggcg atcggaacgc tgacgttccg cacgttgttc agcattttgc
    11401 agccccggat gcccgccttc gacaataacc ccacaaagcc ccgccagccc ctggcaagcc
    11461 gccatcatcg ccacccgtcc gccaagcgag taccccacca gccagaagtc aaggatgttg
    11521 taactaacca aggttttacg cagtaagtcg gtgacatcat caaatccatc gacgctaatc
    11581 gccgccgaac caccatgacc tgggagatca acatacaacc gtgagtagtc ggcaaacgcc
    11641 tcgcccactt cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag
    11701 ggtaaacctg gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg
    11761 ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg
    11821 tggtggttgg cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg
    11881 gacgatgata tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca
    11941 tcagatagaa acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc
    12001 cgttgttgtt caccacaatt aataccagcg gcgcagaaac ctgacgcaat aacgccagcg
    12061 cgttgagatc gtaaagtgcg gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg
    12121 cccgctgaac gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc cctcggttgc
    12181 tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt accaccaggc
    12241 tgttaccaac aaacaattgc ccctgttcag gcagatagtc gcagatgcga tgcgccagtt
    12301 gcgcttcgcc aaacgcatca cggcgggcaa taaccgcctg cattgcctgt tccgccaggc
    12361 gcgggatttc aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg
    12421 caatattggc aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt
    12481 catcaacaat ccagtactct tctggttcac agcttgcctg ccattgcagg agccgtttcc
    12541 ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt
    12601 tggcattgcc taaccaaaga tcggcacacg gcagcggctg cccggtttgt gacagcacat
    12661 cgccaatcag cggccagcca agagtttgcg cccacagggc aacttttttg ccctcttccg
    12721 cactcatgcg cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct
    12781 gtttttcact ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt
    12841 cacccagacg ctgttgccag ctaagcccgg tatcgtccat ttcgccatac agcggttcag
    12901 caaacgggca gttgatatgg actcccccca catgaagcgt accgagagcg tggtcgatgg
    12961 tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg cggcagtgaa atactgtgcg
    13021 tggggtgaga ggcgaacatt cccggctggc gaattgcctg attcgcgccg cagtcaatta
    13081 gctccggcgg gcgatcggcg gttaacagga tcagtttttc tccggttaac ccagcttcaa
    13141 tcagtgccgg atagagattt gctaccgccg tgccggaggt cacaatcacc gccaccggct
    13201 gcttgctgac tttcgccagc cccagcgcca gatgtcccag tccacgctca tcgaaatggg
    13261 tgtggtgaat gaatgtggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc
    13321 caggggcgat acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg
    13381 cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta
    13441 agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac
    13501 tattccatct gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct
    13561 tgctcggggt cggaaccacg gacaatgccc gcgccagcat ataatcgcac gacattgccg
    13621 ctaatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga gagatagccc
    13681 gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc
    13741 agatcgcgcg gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca
    13801 tcatccgctt tgttgagtga agtccagata cagcggcgaa gatgctgcac tttacgcaga
    13861 cgcagtacct gcggcggtaa aacatccagc gtctgggtat cggcctgtaa tcgttggcag
    13921 atatcttcca ccaccagcat gttctcgcgc tggtttttat catccgccat cagccactca
    13981 cctaactgct gcgcctgctt atcatcagga tgatttgcta ccgttcccgc cagcgcttca
    14041 gtacgcagcg cgttgtcacg ccgccgccat aaccgttccg gtgaagagcc aagaaaagca
    14101 gtttcgccat caaaggccat gtaaaaatgg tagcaattca gattcagtcg acgactggca
    14161 gccatcatcg ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc
    14221 accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc
    14281 cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag
    14341 ggcttgatac tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg
    14401 gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg tcgccattcc
    14461 aggcggggta aaagtaaatt gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt
    14521 aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa
    14581 cgggtaatcg cgcccaggac ggcagcttct tcatcaccat tacgttgttg ccagtagaat
    14641 tgcgggtacg tttgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg
    14701 aaaggaatat cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc
    14761 agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct
    14821 cacattatac ggggtactac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg
    14881 cgtgccagca acagtcctag taccagccca acggccgcgc ccacaccaat tccttgccag
    14941 ggtttttcgt ggacgtagtc atcagcacga taaactgcct gcttcgcccg atagtaataa
    15001 ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc tgcacgcgct
    15061 ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt
    15121 gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg attagacatt
    15181 ctcgttctcc atttgcgtaa aacctgtcac taactataga caagggttgt tgattacgcc
    15241 tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata gacctccgtc
    15301 acagggataa aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa
    15361 acaggcttat caggccagtg atgcgtacat gtttcgagtg ttttactcat cagttgctgc
    15421 cccacttttt cgccacgcaa cgcttcactg acaatcaccc gacctataac gaccggctca
    15481 agatcatcat cacttttcag aatcctcgca tatgccacca gttcatcatt tttccaacca
    15541 agaatatggc gattatcccc cgtcaaatca tcaccatcga tatcctgata agggcaattc
    15601 tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg cataaagctg ggaaaccgat
    15661 aattcagagt gatgcagatc ttgccattca atcataatgc tctcctttta gatgtatgcc
    15721 ttatactagc cggaatattt tttgaaacgt gatgaactca tggaattaat ttttttaggt
    15781 acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct taacctgcaa
    15841 catccgaccc agagcggact ttggttgttt gactgcggtg aaggcaccca gcatcagcta
    15901 ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc
    15961 gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa
    16021 cccttaacga tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt
    16081 agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc
    16141 gatgatggcc tgcgcaaagt aaccgcttat ccgctggaac acccactgga atgttatggc
    16201 tatcgtattg aagaacatga taaaccgggt gcattaaatg cccaggcatt aaaagctgct
    16261 ggcgtgccgc ctggtccact gtttcaggaa ttaaaagcgg gcaaaacaat caccctggaa
    16321 gatggaaggc agattaacgg cgcagattac ttagctgctc cagtgccagg taaagcgctc
    16381 gctattttcg gcgataccgg cccctgcgat gccgcacttg atctggctaa aggtgtcgat
    16441 gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc
    16501 catagctcta cacgccaggc tgcgacactg gcccgtgagg ctggagtcgg tatgctaatc
    16561 attacccacg tcagctcgcg ctatgatgac aaaggttgtc agcacctgtt acgtgaatgc
    16621 aggtcaattt tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc
    16681 tgcttattat taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg ctacatatat
    16741 ccacccacac gggcatattg agcgattttt gatatttatt catctcctca cgcactatat
    16801 tcagagcaat aagttgcctc tatttatctt gattcagcac aacctttgca actaaaagga
    16861 gcccatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaacgc aactcagtca
    16921 gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc
    16981 gttatccgga gatgaagaaa gcattgagtt actgcataat attgcgttag gctatgatga
    17041 atttggaaaa aaagctgaag atattcttta ccatattgtt agaaacccaa caaatgagac
    17101 cctatcgatt atccgactta tcaaaaatgc ttgtttaaaa ttatataatt tggcacatgc
    17161 cgcaaccaac tcccccctta aaccagctgg tccggataat tcagaggatc tcctgttcaa
    17221 aaagctattc tccccttcga aattaatgac aattatcggt gatgaaattc ctcttatatc
    17281 tgaaaaacag tcgctttcga aggtgctttt aaatgatgag aataatgaac tgagtgatgg
    17341 tacaaacttc tgggataaaa atcgtcaatt aaccacagat gaaatagatt gctatcttca
    17401 gaagatcgcc gctaatgcaa aaaatactga agtcaattat cctactggtc tctacgtccc
    17461 ctactccacc agaactcacc tggaagacgc tctcaatgaa aatattaaga gcgatccatc
    17521 atggccgaaa gcagtccagt tattccccat aaatactggc ggacactgga tattagtttc
    17581 gctacagaaa atagtaaatg aaaaaaataa tacacaacaa ataaaatgcg tcatattcaa
    17641 ctcattgcgc gcactaggcc atgataaaga aaattcactt aagcgtgtca ttaatagttt
    17701 taattctgaa ttcatgggag aaatgtcgaa taataatata aaagttcatt taactgaacc
    17761 cgagataata tttttacatg ccgatcttca gcaatattta agccaaagtt gcggtgcatt
    17821 tgtgtgcatg gcagcccagg aagtgattga acaaagggaa agcaattctg acagcgcccc
    17881 ctatacgtta ttaaaaaact atgctgacag atttaaaaaa tattcagcag aagagcagta
    17941 cgaaattgat tttcaacata gactggtaaa cagaaattgt tatttagata aatatggcga
    18001 tgcacgaatc aatgcttctt atacacaatt agaaataaaa cactcacaac ccaaaaatag
    18061 agcatccggc aaaagagtga gttaacatca ttgttaatta attcgaccag tcagcaaata
    18121 tgctgactgg tcgaacatag tttgctaaac agtcatcact gactgatgtc agtcacacca
    18181 tccgccagtt caatcttcgg gtatttccag cgtatctcgg cacgcgattg ctggtctgtt
    18241 gatgtagtgg attgttgttg ctgacttttc ttaccctcag agttctgccc cctgtcaact
    18301 caatctgcca ataaataaca taattatatt tttccttata tgaattcctt tttaaaatag
    18361 cgaagccact gtaaaagata ctttttgtat agatctaaca accgtaataa aacaaacact
    18421 cataactcat tataaataat gataaaataa cgtcttttat tgataattta tatatgaaaa
    18481 aaaatccttt tgttatatta tttctgattt cactccctct gattattttt accaaccctg
    18541 ggtcactgca ttatcgccaa cgccccgaca cgtcagtcct gagcaattag aaaaaacagt
    18601 tcgttatctt acacaaactg tgcatccacg taatgccgac aatattgata atctgaatag
    18661 gtccgtagag tatataaaag agttctttgt cagtagcggt gccagagtta ccgcgcaggg
    18721 cgtccccgat tggctattac gactctgcgc ctggtagcca ggattaccct tacccgcaat
    18781 gtcctggctt tatcccgatc ggggggactt tattgcctgg tcggcagaat acaggatatc
    18841 aacgccgttc gtcaggtaaa aacggcattg ttatcatccc gggatttatc tgtttattct
    18901 atgaatgccc catggtttat tcccggtatt gatttctctg accacctgaa ttattggcaa
    18961 cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac
    19021 ttgcccggtg atacagcaga cagattgaat tatcagaaaa tggctcagat ggcgttataa
    19081 ccttgttaca caacagtaaa taataaaaat ttctcgaggc gggtccagaa acagcgacat
    19141 gtacatttta ttttccctcg tctgttttaa gatgcgaaac ataaaattca atggaggaaa
    19201 aataaatggg aatgattggt tattttgcag aaattgactc agaaaagatt aaccaactgc
    19261 ttgagtctac tgaaaaacct ttgatggata atatacatga cacgctttca ggattacgcc
    19321 gactggatat tgataaacgc tgggattttt tacactttgg gctgacaggc acctctgctt
    19381 ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag
    19441 atggcataga tggcttcctg ggattgacct ggaatcagga gttagccgcg acaatcgacc
    19501 gactggaatc actggatcga agtgagctgc gtaaacagtt ttccattaag cgactcaatg
    19561 aaatggagat ttatccggga gtgacctttt cagaagagtt agaggggcaa ctctttgctt
    19621 cgatcatgct tgatatggaa aagctgatta gcgcgtatcg acgtatgctg cgacaaggga
    19681 atcatgcgct gacggttatt gttgggtgat ggtgtcaggg caccaaaaaa aaccgccgaa
    19741 catgtcggcg gctttctgac ttacaaactg atagcttaca tcagcggcat tgctaaccgc
    19801 acaatgctaa tcagcggttg tggccataca cccagcacca gtaccaacag tgcagagatc
    19861 agcaccacga taccgcctgc gctgtactgc cagtttgacg gtgcatcgcg acccggttgt
    19921 tccggggcgt gcaggtacag gctcaccgcc acgcgcaggt agtagtaaag gccgattgcc
    19981 gaaccgacaa ccacggcgcc caccagccac cacaagtgtg cctggacacc gaccgccagc
    20041 acgtagaact taccaataaa gcccagcgtc atcggaatac cggccagaga cagcatcatc
    20101 accgtcatca ccgccgcgag gatcgggcga tgccagaaca gaccgcggta agagaacagg
    20161 gaatcagcat ccgggccacg gtacgggctg gacatcaggc tgaccacgcc gaacgcgccg
    20221 aggctgctga acagataacc ggccaggtaa actcctaccg cttccatcga catctcgccc
    20281 gtttgcagcg caatcagcgc caccagcaga tagccgaggt gagagataga tgagtaaccg
    20341 agcagacgtt tgatattggt ctggctcagc gccatcaggt taccgaagat gatagaggca
    20401 aaggcgataa tcgccagcac tacccgaatc gcttcgctgt cacccaccgg tgcgtagagg
    20461 aacagacgca tcaccacacc gaagatagcg attttgctcg ccgtcgccag gaaagtggaa
    20521 accggcgcag gcgcgccctg gtatacgtct ggcgtccaca ggtggaacgg caccagagag
    20581 agtttgaagc cgaggccaac aatcatcagt ccgaaacctg ccagcaacag cggctcgttg
    20641 agcataccgt cgccgaggtt tttacccagc gcgacaaacg acaggtcgcc agactgtgca
    20701 tacaccagcg ccataccaaa cagcaggaaa gaagacgctg cggcagaaag gatggtgtat
    20761 ttgatactgg cttccagcga acgtttctgg cggaaagcgt aaccgaccag gccaaacagc
    20821 ggcaaagaga tcagttcaat accgaggaac aaagacgcca gatggttggc attcgccagc
    20881 aggatcccgc ccagcgcggc aattaacacc aacaggtaga actcatcctt gttgtcgtta
    20941 tagccttcaa gccacgggta ggcgaaagta caggtggcga ggctcgccaa caataccagc
    21001 ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc catagcgccc
    21061 gcctggccga caaaccagag cgaaaccagc gccgcgttaa gcccaataac cgagagcgta
    21121 gcgttgagga aatgattgcg tcgccacgca atggagagca tcacaaccac caccgtcaag
    21181 ccgacgatca gcaacggtag cagtgcgatc aggttttgtg gagttattgt catggcgatt
    21241 tacggccttg tagtagtaac ggaattaaca aaccactgct ggatattgcc aatcgcggag
    21301 tgcgaggtat ccagaatcgg ctgcggatag aagcccagca gtaccagcag caccaccagc
    21361 aacaggatca taaacagctc acgcagcgac atccctggca gttcctggct ggcaatctgg
    21421 cttttcgctt taccgaagta agcgcgatgc aacatcgcca gcgaataaac agatgcaaag
    21481 accagcccaa aggtagagat aacggtaatc accgggacaa cctggaagct gccgaacaga
    21541 atcataaatt cgccgacgaa gttaccggtg ccaggcatcc caagcgttgc caccgcaaag
    21601 aacagcgaca gtgctggcag ccatttcatc ttgctccaca gaccgcccat catgcgcatg
    21661 tcgcgggtat ggatacgttc ataaagctga ccacaaagaa taaacagacc cgccgccgac
    21721 aagccgtgcg caatcatctg gattaccgcg ccctggtagg ccaactggct gccggtgtag
    21781 atagcaatca gcacgaagcc catgtgggaa accgaggtgt aggcgatcag acgtttgata
    21841 tcggtctggg cgaaagccat ccacgcaccg tagaagatgc cgataacccc cagccacata
    21901 gcgattggcg caaactctgc cgatgcgttc gggaacagcg gcagggagaa acgcagcaaa
    21961 ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa cggaaccggc ggtcggagcc
    22021 tgagagtgcg catccggtag ccagccatgc agcggaacca ccggcatttt gactgcgaag
    22081 gcgatgaaga agcccagcat taacaggtat tccacaccac tggacattgg cgtattcagc
    22141 agctcttcat agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc
    22201 aggatggcaa tcaacatcac caggccactc gcctgggtgt aaatgaagaa cttggttgcc
    22261 gccgtgatac gcgttttacc gtcagaggct ttatgccccc acagtgcgat caggaagtac
    22321 atcggcacca gcatcatttc ccagaagaag aagaacagga acatgtcgat ggcaaggaac
    22381 acgccgataa cgccgcccag gatccacatc aggttgaggt ggaagaagcc ctgatatttt
    22441 tcgatctctt tccacgaaca tagtaccgcc agcacaccga gcagaccggt cagcacgacc
    22501 atcagcagcg acagcccgtc aatggcgaga tgaatagaga taccaaaacg cgggatccac
    22561 ggcatgtcga attcagactg ccactgcgga attccggcgg attgcgtcag tgaataaccg
    22621 ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc
    22681 cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc acaggaagcc gccaataaag
    22741 ggaattaata ttagccaggg tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc
    22801 aggaccggat tttcaacgaa ttctcacgac agaaaattct cttcggactg ggggtagtgt
    22861 cggatgcacc gcgaaggcgg cttatccgac ctacgttctt atatgcccag gggcaatccc
    22921 acaatcctta actcaacgca gtaccatcaa cagtgccagc accacgaccg caccgatgct
    22981 catggatgcc acataccagc gcagatagcc gttctcactt aacagcagac ctttacctgc
    23041 aaagcgggaa aggacagccg ggatgttcat cattgagttc agcggatcgc gtttcagcaa
    23101 ccaggcaata cccaggaacg gcttgacgaa cactttgtca tacagccagt caaatcccca
    23161 ggcgttgtac caccaggtgc ccagcagacg gcccggcgca ctgttggcga tggaggtcac
    23221 cagagtacgt ttacccagcc acagccaggc tgccagcaga atgccgacca ccgcgaccac
    23281 gccagaggta atttccaggg tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag
    23341 cacgccctgc agcggcggta caatcagtgc gccaacgaag gtggaaagga tcagcagcac
    23401 aatcagcggc aggctgtgag ttaccccttt cacggcgtga gcgtgaattt gttcttttcc
    23461 gtggaagacg atgaaaatca tacggaaggt gtagagcgag gtcataaacg caccgaccag
    23521 acctgccacc atcagattga tatgaccatt cgccatcgca cccgcgagga tctcatcctt
    23581 actgaagaag cccgcagtga ccagcggtag tgccgacagt gctgcgccgc ccaccaggaa
    23641 gcagagataa accagcggaa tagatttacg cagaccgccc atcttgaaga tgttctgttc
    23701 gtgatggcag gccagaatga cggaaccgga tgccaggaac agcagcgctt taaagaacgc
    23761 gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat
    23821 gtagccaatc tggctcatgg tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag
    23881 cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca atacccacca gatgcagaac
    23941 ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc aggtagacac ccgcggttac
    24001 catcgttgcg gcgtggatca gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag
    24061 ccatgtctgc aacggcaact gcgcagattt accgaccgca ccgcccagca gcatcagcgt
    24121 cgcccacatc agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc
    24181 gcggaagttc agggtgccca gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac
    24241 gtcacccaca cgggtcacga cgaacgcttt cattgccgct gcgccattct tcggatcggt
    24301 gtaatagaaa ccgatcagca gataggagca caggcccacg ccttcccagc cgaggtacat
    24361 cagcagcagg ttatcggcga gaaccagaac taccatgctg gcgatgaaca ggttggtgta
    24421 ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac caggaggcgt acatgtgaat
    24481 aaggaaaccc acaccagtga ccaccgagag catggtcagc gacaggccgt ccagcaccag
    24541 gttaaaaccg atgttaaagt cgcctaccga catccacgtc cacagcggct ggctgtatga
    24601 ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc
    24661 cacagagcca acgccgacga tcgccgaaac gttttcagac cagcgcccac gggagaacgc
    24721 cagcaggacg aagccaatca atggcaaaat aatggttaag gcaagcatgt tcatccgcgc
    24781 atctcactta ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg cagcagcagc
    24841 gcaaggccga tactcgcttc tgccgccgcg aggctgatgg cgagaatgta catcacctga
    24901 ccgtcggtct gaccccagta gcttccggcg accacaaagg ccagcgcgga ggcgttaatc
    24961 atgatttcca gaccaatcag cataaacagc agattgcgac ggataaccag accggttaag
    25021 ccaagaacga ataagattgc cgcgaggatc agtccatgtt gtaaggggat catgcgtgct
    25081 cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct cagcacttca cccgcacgct
    25141 cttcacgacc gacgtggaag gccacaacca gacccgcgag cagcagcata gaagccagtt
    25201 ccaccgccag tacgtaaggc ccgaacagcg taatacccac tgctttagca ctgattggcg
    25261 taccgtcgat accctgatca ttaacaccaa ggatggcgta aacaatcacc accagcatga
    25321 tggccgacaa aattgccgga ccaatccaca cctgcggttt cagccactgg cgttcctgtt
    25381 cgatttctga accgcccagg ttgagcatca tcaccacgaa cacgaacagc accataatgg
    25441 cacccgcgta gacgataatt tccagcgcac cggcgaagta agcgcccagt gagaagaaca
    25501 ccccggaaat cgccagcagc gaaataatca ggtacagcag tgcgtgtacc ggattggtat
    25561 gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa aaagcgaact
    25621 ccattgcccc tctccttacg gtaacaggct cttgacgtcg ataggcttgg cttcgttctc
    25681 tgcttcgccc ttatctttgc cgtcgattgc catacctgcc atccggtaga agttatattc
    25741 cgggtatttg cccggaccgg agatcagcag atcctctttt tcgtaaacca gatcctggcg
    25801 cttgtattcc cccatttcga aatccggggt taactgaatc gccgtggtcg gacaggcttc
    25861 ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt ccgggtacca
    25921 gcgaccgtct ttggtttctg ctttttgcag cgagatacag ccgaccgggc aggctaccgc
    25981 gcagaggtta caggctacgc aacgctcttc gccgtccggg tcgcgggtca gaacgatacg
    26041 accacgataa cggggcggca gatagaccgg ctcttccggg tacattcgcg tttcgcgttt
    26101 ggcgaacgcg tgcaggccga tcatccagat actacgaacc tgggtgccga aacctactaa
    26161 caattctttt aaggtcatgg tcttattgcc ccttattgcg cctgccagag aatgacagcc
    26221 gccgttacca gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt
    26281 acctggtcat aacgcggacg cggtaacgac gcacgaatca aaatgaacat catcataaag
    26341 aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca acgggccttg ccagccaccg
    26401 aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc cgatgtactc acccacgaag
    26461 aacagaccga acttcatacc ggaatattca atgtggtaac catccgccag ttcctgctcg
    26521 gcttccggct ggtcaaacgg gtgacggtga cataccgcca cgcccgcgat ggcaaaggta
    26581 ataaaaccaa agaattgcgg gataacgttc cacacatgcg cctggctgtt gacgatgtcg
    26641 gtcatgttga atgaaccggc ctgcgccacc acgcccatca gggaaagccc gaggaacact
    26701 tcgtagctca gggtctgcgc agaagcacgc atcgcaccca gcaacgagta tttgttgtta
    26761 cttgaccagc ccgcaaacag caccgcgtaa accgccagac ctgccatcat caggaagaac
    26821 aaaatcccga tgttcaggtc ggcaaccacc caacccggac tgactggcac aatcgcaaag
    26881 gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg taaagatgac gcgatccgag
    26941 aatttcggga tccagtcttc tttaaagaac attttgatca tgtccgcaac cagctggagc
    27001 gaaccgcccc agccaacacg gttaggtccg taacggttct ggaacagacc cagcaggcga
    27061 cgttcgccaa agctcatgaa tgccccgcag gtgacaacca ccagcaggat caccaccgct
    27121 ttgaggatgg tcagcaggat ctcaatcagt tccggtgata tccaactcat tgttgtgcct
    27181 ccttgagatc ctcaagatgc gcgccagcca gcaccggagc aataccggac atacccatcg
    27241 gcaagcccac ctgccctgcc gtcagccctt cagcgatttc aaccggcagc gtgaccgtgt
    27301 tgccatcgta actaaaggag acgtgtgtac ctgcgttcac acctaacttc gcggcatccg
    27361 ctgggttgag tttgatgtac ggctgcggca tacggctctg gaagaccgga gcacgctgtg
    27421 acaattcatc gctgccaaac aggtggtaat acggcgcgat acgccatttc ccgtcctgcg
    27481 gctggaagcg tgccggtacg ctggtgaagt aatccagacc attttcgctg gtttcaaaca
    27541 gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc
    27601 acgcctgcgg ggagttccag cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg
    27661 tcggctggtt gttaccttcc atcgagaagg tgaacatggt gtcaatatcc tgcggctgac
    27721 gcggctcatg aacgctgata ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt
    27781 cacgggccag tttctgccca cgaatacgga atgtcgcatc cggcgcagca tctttgatac
    27841 ctgccagttc cgggattttc gccacaacag cgtcaatcac atggtcgagc tgcgtccagt
    27901 ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa ccagcgccag ctttccagca
    27961 tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg gaagaaacgt tgggcgcggc
    28021 cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct ggcagcagaa agtaccagat
    28081 gggcgttttc cataatcgct gtgcgttgat gatcaaccac catcaccagc ggcgctttag
    28141 ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg cagatcgttt tccagcacca
    28201 ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc ttcttcaagc gaaccgccac
    28261 ccataatgcc cagccccatg ctgttgacgg aacgggcaat catggtgata ccaacgtcag
    28321 cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac
    28381 cggcgttcgt cccggagata atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga
    28441 cgtcgatttt gctttgcagc tcaggttcga taccgtcaac cgctggcgca gagttatcca
    28501 gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc
    28561 acgccgcgat atcatccaga cgggtgtcat caacgttggt aacaaacagc ggatgcttcg
    28621 cacgttgacc gatgttgagg attgccgcaa tctgccagtc agccactttc tgtgctgccg
    28681 ccatttcgcg cgctttacct ttcacagcct gacgcactgc cagcgcgacg cgcgcgccgg
    28741 tctgggtaac gtcttcgccc agcaccagta ccgcatcgta agattcgatt tcgcgcagag
    28801 ccggagtata aatgccgcct tcacgcagca ctttcagcgc cagttgcaga cgttcctgct
    28861 caccgtgagc gataccggtg tagaagtttt cttcgcccac cagttcacgc agcgcaaagt
    28921 tgctttccac gctggcacgc ggagaaccaa taccgatcac tttcttcgac tgacgcagaa
    28981 tatctgccgc gccctgcatt gcctgttcgg cgttgagggt aatgaaatca tcgccacgac
    29041 gctgtactgg ttgacgcgga cgatctttca ggttgacgta accgtaaccg aaacgaccac
    29101 ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg gttttcgata cgacgcagtt
    29161 cgccgtaacg ttcaccgggg ctgatgttac agccgatgga acattgctgg cagatgctcg
    29221 gcgcaaactg catatcccat ttacggttgt aacgctcgga gtgcgttttg tcggtaaata
    29281 cgccggtcgg gcaaatttcg accaggttac cggaaaattc gctttccagc gtgccgtctt
    29341 ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac gcccagatcc gtaccgtcag
    29401 cgtaatcttt gtagtaacgc acgcagcggt agcaggcgat gcagcggttc atttcgtgag
    29461 agatgaatgg ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg tagcgacgga
    29521 agctgtgtcc ggtcatcacg gtcatatcct gaagatggca gttaccgcct tcttcacata
    29581 ccggacagtc gtgcgggtgg ttggtcatta accactcgac cacactttca cggaactgtt
    29641 tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc atacaggaca
    29701 tcaccaggcg accacgcgtg tcttccgcgt tttggtattg cttcaccgca cactggcggc
    29761 aagcaccgac gcttcccagc gccggatgcc agcaaaagta aggaatatca aggcccagag
    29821 acagacaagc ttccagcagg ttgtccgctc cgttgacctc gtattctttg ccgtctacat
    29881 gaattgtagc cattagcatg cttccagttt tctcagtcag agactgagcg ttaatcgaaa
    29941 ttcggttacc agcgctcttt cagcaggttc ggctgaatcc cattaatcaa atgggtattg
    30001 ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga aatatttgat agcgctctgt
    30061 aacggctcca ctgcaccagg tgcgtgggca cagaaagttt tacccgggcc taagaatcga
    30121 cacagttgct caagtgtttc gatatcgccc ggctgacctt caccacgctc cagcgcacgc
    30181 agaattttca cgctccacgg cagaccgtcg cggcacggcg tacaccagcc gcaggactca
    30241 cgggcgaaaa actcttccag gttacgcacc agcgacacca tgttgatctc atggtcaacc
    30301 gccatcgcca gcgccgtacc cagacggctg cccgctttac cgatactttc gaattccatc
    30361 ggcagatcaa ggtgcgcttc ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct
    30421 ttaaatttca gaccatcacg cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg
    30481 gtgccgaacg gcagttccca cagtcccgga tttttcaccc gaccggagaa gcccatcagc
    30541 ttggtgccag catctttact tttcgagatg ttctgatacc actccacgcc gttagcgagg
    30601 atcgccggaa cgttacacag ggtttcgacg ttgttgacac aggtcggttt gccccatacg
    30661 ccggaggttg ccgggaaggg gggtttcgag cgtgggttag cacgacgccc ttccagggag
    30721 ttgattaacg ctgtttcttc cccgcagatg tagcgccctg ccccggtatg gacgaacagt
    30781 tcgaagtcga aacctgttcc cataatgttt ttgccaagca agcccgcttc agtggcttcg
    30841 gcaatggcgc ggcgcagatt tactgccgct tcgatatatt cgccacgcag gaagatgtag
    30901 ccacggtaag ctttcagcgc aaacgcggag atgagcatac cttccaccag caggtgcggc
    30961 agttgctcca tcagcaggcg gtctttatag gtgcctggct ccatttcatc ggcgttacac
    31021 agcaggtaac ggatgttcat ggattcgtct ttcggcatca ggctccattt caggccggta
    31081 gaaaagcccg cgccaccgcg ccctttcaga ccagcgtctt ttacctgatt aacgatttcg
    31141 tccggagaca gcccggtcag cgccttacgc gcgccttcgt aaccgttttt gctgcgatat
    31201 tcgtccagcc acactggctg tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg
    31261 ggagtacgga taatgttttt catttatacc gctccagcag ttcagggatc gcttccgggg
    31321 tcagatgcgc gtgagtatcc tcatcgatca tcatgtttgg ccctttatca cagttcccca
    31381 ggcagcaagt tggcagcagc gtaaagcgac cgtcaaatgt cgtttgccct ggtttgatgt
    31441 ttagcttttt ctcgagcgcc gcctgaatac cctggtaacc gttgatatga cagaccacgc
    31501 tgtcgcaata acggatcaca tggcggccaa ccggctggcg gaagatctgg ctgtagaacg
    31561 ttgccacacc ttcgacgtcg cttgccggaa tacccagcac atcggcgatc gcgtggatcg
    31621 caccatccgg cacccagcca cgctgcttct gaacgatttt cagcgcttca atggacgccg
    31681 cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc ttcacgctct gccgcactca
    31741 gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat aattagcggt ccacatctga
    31801 cataacaaaa tcgatactgc ccagataaac aatcaggtca gacaccaggc taccgcggat
    31861 caccgccgga atttgctgca aatgcgcata actcggcgtg cggatacgag tgcgataact
    31921 catggtgcta ccgtcgctgg tcaggtagta actgttgatc cccttggtcg cctcaatcat
    31981 ctggaaagat tcattggcag gcatcaccgg cccccacgac acttgcagga agtgggtgat
    32041 cagggtttcg atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc
    32101 cgctttgaac gggccttccg gcatgttgtt gaggcactgc tcaagaatgc gcagactctg
    32161 gcgcagctct tccactttca gcattacgcg ggtgtagcag tcagaaacac caccaccaac
    32221 cgggatttca aagtcgaagt tttcatagcc agaataagga cgcgccttac gcacgtcgaa
    32281 gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc cactccagcg cctctttcgc
    32341 gccataggcg gcaacgccct gggaacgacc tttcagaatg gtattttgca gcgccgcttt
    32401 ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac tcacgcagca ggcgatccca
    32461 gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt gcatacggaa
    32521 acccgtgatc gcttccacca gatcgtaaat tttctgacga tcggtaaagg cgaagaacac
    32581 gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg tacagcaggt ggctgttgat
    32641 acggaacagt tcggagagca taacgcgaat gacgttaacg cgatccggca cggtgatccc
    32701 ggccagtttc tctaccgcca gcacgtaagg catttcgtta acgcagccgc cgaggtattc
    32761 aatacggtcg gtatacggaa tgtagctgtg ccaggactgg cgctcgccca ttttctccgc
    32821 accacggtgg tggtagccga tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg
    32881 taaaacgata cggaaagccc cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat
    32941 gaagtcctcg ttttcggtgc cgcgcttcat cccccactct tccggtttga aggtcagggc
    33001 ttccatctcc agatcctgtt tggctttggt cagctcaaac ggcgagaatt cggtagcgcg
    33061 cgccgggtaa tctttacgca gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg
    33121 caggttcggg tgaccgtcga aagtaatgcc aaacagatcc caggtttcac gctcatacca
    33181 gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc
    33241 caccttcagc atgatgtcgc ggttacgatc gatagaaatc agatggtaga aaacggaaaa
    33301 atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc
    33361 aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt ccagtaattg
    33421 ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc
    33481 cggcccaaaa cggttgcgca gttcgccaat caccggatca tcaagatgat cgcgggtctg
    33541 ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc accattgcaa atggtccgtg
    33601 gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc
    33661 tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg
    33721 ttcaccgcgc ttgcgttcgc gctctgattg catattggcg cgataaacgc cctgatcgcc
    33781 aaccacccag gagagcggac gacgttcttt gccgatagat tcctgcaaca gcatcagtgc
    33841 ctgcatgtac gcttcaggac gcggcgggca gcccgggata tacacatcaa ccgggatgaa
    33901 tttatcgacg ccctgcacaa cggaataaat atcgtacata ccaccagagt tggcacaggc
    33961 acccattgag ataacccatt ttggttccag catctggtca tacagacgct gaataaccgg
    34021 tgccattttg gtaaagcagg ttcctgcaac caccatcagg tcagcctgac gcggcgaagc
    34081 acgcaatact tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat
    34141 ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa attgagtttt tacgacccca
    34201 gttaaccatg tcattgagct tgcccataaa cacgtttttg ttaacttctt gctccagagg
    34261 gtcggttacg atctcctgct tttgcagggg gtaacggtcg ttctcaccgt tgggatctat
    34321 gcgggtgagc gtataatcca tcttaatgcc tcgcggttag cgttgacgat tagcgatact
    34381 gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc
    34441 aatacgcacc agataaacca gacctgccag taacacaaaa ataaaaattg cagcttccac
    34501 aaagcctacc cagccgcttt cgcggataga ggttgaccat gcgaacagat acagcgcttc
    34561 aacgtcgaag ataacgaaga acatggccac cagataaaac ttggcggaca ggcgtaagcg
    34621 ggcggagccg accgagtcga taccggattc aaacggcacg tttttcgacc tcgcgcgtgc
    34681 gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa cacaggccaa tggcaacgat
    34741 aagaaagata gcgaatgccc agtgatgagc gatgacttca gtggatgttg acatactcat
    34801 tgcttactca tcaaaagtag cgccagattc actgctcttc acggcagatg gacgccacat
    34861 cgattcatgg ggaggaataa aaaaaacctt acaatcactg tagaaattct tttatacagc
    34921 taattgatgt ggtcttttac tcctttctat aaccttttgt caactttaac aaaagtttct
    34981 tcacattagt ttacataata tcaacaccat tagcatttaa tgccctttca ccccagatcc
    35041 ttgacgactc caggataatt agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa
    35101 aatacgggtc tattttgaca ggaatttgtg tcgattcctc cccccaaaag agagtatttt
    35161 cttgatctgt gacacgcttt tgtcattcca taacaaaaac gcagcaacaa atttacgtat
    35221 tttttaacat cattgtagca ggtgattttt ttcaggcgat tatttgtgcg ttcgggacgt
    35281 gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc tgcactatgc gaggaaggat
    35341 ttttactaaa aaaaagccgc tggggtttaa aacaccccca gcggctcgtt ttttacacta
    35401 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    35461 atcatcccct tccggagcag acattgggct gtattgccac ggattatggt agctctccat
    35521 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    35581 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca ggtccgggct
    35641 catcatctca accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    35701 cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat taagcgtcgc
    35761 cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcgcc
    35821 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggttc gcaggttcaa
    35881 tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    35941 catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac
    36001 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    36061 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    36121 acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    36181 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    36241 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    36301 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc
    36361 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    36421 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    36481 aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc
    36541 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    36601 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    36661 gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    36721 agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggtc
    36781 attgcgaatt atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa
    36841 t
//
LOCUS       DAMZII010000038        34050 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZII010000038 DAMZII010000000
VERSION     DAMZII010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.038"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            11..751
                     /locus_tag="POA77_001824"
     CDS             11..751
                     /locus_tag="POA77_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6365270.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            786..3386
                     /gene="htrE"
                     /locus_tag="POA77_001825"
     CDS             786..3386
                     /gene="htrE"
                     /locus_tag="POA77_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDI6365271.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3403..3972
                     /locus_tag="POA77_001826"
     CDS             3403..3972
                     /locus_tag="POA77_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6365272.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3987..4589
                     /gene="yadL"
                     /locus_tag="POA77_001827"
     CDS             3987..4589
                     /gene="yadL"
                     /locus_tag="POA77_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDI6365273.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4616..5212
                     /locus_tag="POA77_001828"
     CDS             4616..5212
                     /locus_tag="POA77_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6365274.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5265..6539
                     /locus_tag="POA77_001829"
     CDS             5265..6539
                     /locus_tag="POA77_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDI6365275.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            6652..7446
                     /gene="panB"
                     /locus_tag="POA77_001830"
     CDS             6652..7446
                     /gene="panB"
                     /locus_tag="POA77_001830"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI6365276.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7458..8309
                     /gene="panC"
                     /locus_tag="POA77_001831"
     CDS             7458..8309
                     /gene="panC"
                     /locus_tag="POA77_001831"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6365277.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8383..9285)
                     /gene="rpnC"
                     /locus_tag="POA77_001832"
     CDS             complement(8383..9285)
                     /gene="rpnC"
                     /locus_tag="POA77_001832"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDI6365278.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSRKDVAEMTNLSLAEIDKVINLI"
     gene            9559..9939
                     /gene="panD"
                     /locus_tag="POA77_001833"
     CDS             9559..9939
                     /gene="panD"
                     /locus_tag="POA77_001833"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI6365279.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9943..11172)
                     /locus_tag="POA77_001834"
     CDS             complement(9943..11172)
                     /locus_tag="POA77_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI6365280.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11236..11676)
                     /locus_tag="POA77_001835"
     CDS             complement(11236..11676)
                     /locus_tag="POA77_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6365281.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11781..12551)
                     /locus_tag="POA77_001836"
     CDS             complement(11781..12551)
                     /locus_tag="POA77_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6365282.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12548..13474)
                     /locus_tag="POA77_001837"
     CDS             complement(12548..13474)
                     /locus_tag="POA77_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6365283.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13583..14245
                     /gene="can"
                     /locus_tag="POA77_001838"
     CDS             13583..14245
                     /gene="can"
                     /locus_tag="POA77_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDI6365284.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14286..14822)
                     /gene="hpt"
                     /locus_tag="POA77_001839"
     CDS             complement(14286..14822)
                     /gene="hpt"
                     /locus_tag="POA77_001839"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI6365285.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15028..17418
                     /gene="gcd"
                     /locus_tag="POA77_001840"
     CDS             15028..17418
                     /gene="gcd"
                     /locus_tag="POA77_001840"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDI6365286.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17465..19015)
                     /gene="cueO"
                     /locus_tag="POA77_001841"
     CDS             complement(17465..19015)
                     /gene="cueO"
                     /locus_tag="POA77_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDI6365287.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19181..19528
                     /locus_tag="POA77_001842"
     CDS             19181..19528
                     /locus_tag="POA77_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDI6365288.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19634..20500
                     /gene="speE"
                     /locus_tag="POA77_001843"
     CDS             19634..20500
                     /gene="speE"
                     /locus_tag="POA77_001843"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDI6365289.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            20516..21310
                     /gene="speD"
                     /locus_tag="POA77_001844"
     CDS             20516..21310
                     /gene="speD"
                     /locus_tag="POA77_001844"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDI6365290.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21348..21710)
                     /locus_tag="POA77_001845"
     CDS             complement(21348..21710)
                     /locus_tag="POA77_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDI6365291.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(21885..24482)
                     /gene="acnB"
                     /locus_tag="POA77_001846"
     CDS             complement(21885..24482)
                     /gene="acnB"
                     /locus_tag="POA77_001846"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDI6365292.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            24837..26594
                     /locus_tag="POA77_001847"
     CDS             24837..26594
                     /locus_tag="POA77_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDI6365293.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(26665..28089)
                     /gene="lpdA"
                     /locus_tag="POA77_001848"
     CDS             complement(26665..28089)
                     /gene="lpdA"
                     /locus_tag="POA77_001848"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6365294.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28414..30306)
                     /gene="aceF"
                     /locus_tag="POA77_001849"
     CDS             complement(28414..30306)
                     /gene="aceF"
                     /locus_tag="POA77_001849"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDI6365295.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30321..32984)
                     /gene="aceE"
                     /locus_tag="POA77_001850"
     CDS             complement(30321..32984)
                     /gene="aceE"
                     /locus_tag="POA77_001850"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDI6365296.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLKRMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33145..33909)
                     /gene="pdhR"
                     /locus_tag="POA77_001851"
     CDS             complement(33145..33909)
                     /gene="pdhR"
                     /locus_tag="POA77_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDI6365297.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8515 a         8871 c         8217 g         8447 t
ORIGIN      
        1 gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg taacctgtat
       61 ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg taatatataa
      121 aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc cgttgcttgt
      181 ccagagttgg ttagatactg gcgatgacaa cgctgagcct ggcagtatta cagtcccttt
      241 tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat
      301 gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt ttaacgtact
      361 ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc
      421 atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa atccctctga
      481 agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt
      541 gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag ctagcggtaa
      601 acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca tgaaagtcac
      661 tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta atgattttgg
      721 tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc cgcaggaagc
      781 ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt atcgctacgt
      841 tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa tatgaccata
      901 ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt gaaggtaacc
      961 ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct attattaacc
     1021 aaagcattac atttattgaa attgaaggaa aaaaaaacgc acaagcctgt atcacactta
     1081 agaatttatt gcagtttcat ataaattctc ccgatataaa taacgaaaaa gccgttctgc
     1141 ttgccaggga tgaaacactc ggcaattgcc ttaatttgac ggaaattatc cctcaggctt
     1201 ctgttcgtta tgacgttaac gagcaacgtc tggatataga cgttcctcag gcatgggtaa
     1261 tgaaaaatta ccaaaactat gttgatccat cgttatggga aaatggcatt aatgcggcca
     1321 tgttgtcata caacctcaac ggatatcata gtgaaactcc tggtcgaaga aatgacagca
     1381 tttatgctgc ttttaacggt gggatgaatt taggtgcatg gcgactgcgt gcctcaggca
     1441 actacaactg gatgaccgat tctggcagta attatgattt taagaatcgg tatatacagc
     1501 gtgatatcgc ctcgctgcgt tctcaactca ttcttggtga gtcttataca acgggcgaaa
     1561 cctttgattc tgtcagtatc cgcggcattc gtttatacag cgacagccgc atgttgcctc
     1621 cgacattagc gagttttgcg cctatcattc atggcgttgc taataccaac gccaaagtca
     1681 ctattacgca aggtggctat aagatttatg aaacgactgt gccgccaggc gcgttcgtca
     1741 ttgatgatct gagtccgtca ggttacggca gcgatcttat tgttactgtc gaagaatccg
     1801 atggctcaaa gcggacattc tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg
     1861 gcgttggacg ttgggatatt agcggcggcc aggtcttaaa agatgatatt caggatgagc
     1921 ctaatttatt tcaggccagc tactactacg gtctgaataa ctatctaacg ggttatactg
     1981 gtattcagat taccgataat aactataccg ctggtttgtt aggtcttggt ctgaatactt
     2041 cagtcggtgc attttctttc gatgtgactc actccaatgt tcgtataccg gatgataaaa
     2101 catatcaggg acaaagttat cgtgtttcct ggaacaagtt attcgaagaa acaagtactt
     2161 cactgaatat cgcggcctat cgctattcga cacagaatta ccttggtctt aatgatgcac
     2221 taactctaat tgatgaagtg aaacatcccg aacaagatct tgaaccgaaa tccatgcgta
     2281 attactcgcg catgaaaaat caggttacgg tcagtattaa ccaaccgcta aaatttgaga
     2341 aaaaagatta cggttcattt tatctttccg gaagttggtc cgattactgg gcttccggac
     2401 aaaatcgtag caattactct attggctaca gtaacagtgc atcctggggc agctacagtg
     2461 tcagtgccca gcgttcatgg aatgaagacg gcgacactga cgatagcgtt tatctcagtt
     2521 tcaccattcc tattgaaaaa ttacttggca ctgaacaacg gacttcaggt ttccagagta
     2581 ttgatactca aatgagcagt gactttaagg gtaataacca actcaacgtc agtagcagtg
     2641 gctatagtga taacgctcgc gtcagttata gcgtaaatac tggctatacg atgaataaag
     2701 ccagcaaaga tttgagttat gttgggggtt atgccagcta tgaatcaccg tggggaaccc
     2761 tggcaggttc agtttctgca aatagcgata acagtcgtca agtgtctctc agcaccgacg
     2821 gtggttttgt attgcatagc ggcggcctga ccttcagtaa tgacagtttt agcgactccg
     2881 atacactggc ggtagttcag gctccaggtg ctcaaggagc gcgaataaat tatggcaaca
     2941 gtactatcga tcgatggggt tatggtgtca ccagcgctct ttctccttat catgaaaacc
     3001 gtatcgcgct ggatatcaac gatcttgaga acgatgttga attaaaaagt accagtgcag
     3061 tagctgtacc gcgtcagggt tcagtcgtct ttgctgattt tgaaaccgtg caagggcaat
     3121 cagccattat gaacatcaca cgaagtgatg gtaaaaatat tccatttgct gcagatattt
     3181 atgatgagca aggcaatgtc attggtaatg ttggacaggg tggacaagca tttgttcgtg
     3241 gtattgagca gcagggaaat atcagcatta aatggctcga agaaagtaaa cccgtaagtt
     3301 gtcttgcgca ttatcaacaa agttcagaag cagaaaaaat agcgcaatct attattctga
     3361 atggaattcg gtgtcagatt cagtaactac aagggacgtc aaatgataaa aataacgcca
     3421 cataaaatag cgatactgat gggaatatta ttatcaccat cagtatttgc aacggatatt
     3481 aatgtagagt ttacagccac tgtcaaagcg acaacctgta acatcacact tactggtaat
     3541 aacgtcacga atgatggcaa taataactac acattgagaa tccctaagat gggtctggat
     3601 aagatcgcga ataaaacgac agaatctcag gctgatttta aactggttgc tagtgggtgc
     3661 agcagtggca tcagttggat tgataccact ctgaccggaa atgcatcatc aagctcacct
     3721 aagcttatta taccgcagtc tggtgattca tcttcgacga caagtaatat cggtatgggt
     3781 ttcaaaaaac ggactactga tgatgccact ttccttaaac ctaacagtgc ggaaaagata
     3841 cgctggagca cagacgagat gcagcccgat aagggtcttg aaatgaccgt tgcgctacgt
     3901 gaaacagatg cagggcaagg cgtaccgggg aattttcgtg cactggcaac gtttaatttc
     3961 atctatcaat aagaaggata attaagatga catttaaaaa tttacgttat ggattatcca
     4021 gcagtgtcgt tttggctgcc tcattgttca gcgtaccctc ttatgcggca acagacagca
     4081 ttgggctgac cgtgattact accgtagaaa taggtacttg tactgctaca ttagtaaatg
     4141 actctgatca ggacatttct gttgttgact ttggcgatgt atatatttct gaaatcaatg
     4201 ccaagaccaa agttaaaaca ttcaaactca aattcaaaga ctgtgcgggt atccccaata
     4261 aaaaagcaca aataaaatta accaagcgag ccacatgcga aggtactgct aatgacggtg
     4321 cggggttcgc aaatggatcc acagccgcag ataaagcaag tgccgtcgcc gttgaagtct
     4381 ggagcaccga aactccggca acaggtagcg caacacaatt tagttgtgtg acaccagcat
     4441 ctcaagaggt cactatctcc aatgcagcca acgcggttgt ttattatccg atgagtgctc
     4501 gactggtcgt cgaaaaaaat aaaaccgtaa gcaatgtcac tgcgggtaaa ttttctgcac
     4561 ctgccacatt tacagtaacc tataactaac agactgacag atccaggaac tatatatgca
     4621 tcccactcaa cgtaagctga tgaagagaat aattctgttt ctgtcactac agtttcccat
     4681 tacctatcca gccatcgctg ggcaggatat tgaccttgtt gccaatgtaa aaaacagcac
     4741 ttgcaaaagc ggaatcagta accagggtaa tattgatctt ggcgtcgttg gggtgggata
     4801 tttttcagat aatgttactc ctgaaagcta tcaaccaggt ggaaaagagt tcactgtcac
     4861 agtatcagac tgtgcattac agggaactgg cgatgtgctt aatcagttac atattgattt
     4921 tagagccctt agcggtgtca tggctgcagg ttcaaggcaa atatttgcca atgaagtcgc
     4981 aaccggggct aaaaatgtcg gtgtcgttct cttttccatt caggacccga ccaatatatt
     5041 caatgttatt agttcagcag gaaattctcg ttccgtgtat ccagtaatga cttctgccct
     5101 gcataattct tcatggaaat tttatgccag aatgcaaaaa atcgatcccg cgctggatgt
     5161 catatctggc caggtaatga gtcatatatt agttgatgtg tactacgaat aatagatcaa
     5221 aacaaataac tgcgactgtt acagcagtag atggtggagt gataatgaag aaattcttca
     5281 gacattttct ttttttaatt ctgtgcttga gttgttatac cgcgagtgct ggcactgatg
     5341 ataacgtagg atatattgtt gggaatagtt acggcgttgg tccgtcagat caaaaatgga
     5401 gagaaacggg gccgaatggc gatgctactg tcatattcag atatgcaaca agtacaaata
     5461 atttagtttt ctacaaaccg acgcagcttg gacctacagg tgtaaaatta cagtggagtc
     5521 agttagatac cgcttctggt ggtggttttc tttattgcaa cagaagtgat agcacaagtg
     5581 gtagcgcaat gcgtattgaa aatgcaatgg ttgactcagg taaaatgtat ggctcccata
     5641 aattatttaa tacatcagtt cctggtttgt attacacatt attaatttca aacatgtggt
     5701 cagcttacgg taccgtaact aacgttagtt cacctgggat atacattggt gactctgcag
     5761 aacaatattt ttcgtggtat aatccaagcg aagacgtgtt atattggagt tgcaataatg
     5821 cgaatagcac ccgtaaatac tgggctgtag gtggtattta tcagaccctt acaattgaat
     5881 tctatacaga tacaaacttt gatccaacag taactcaaca gatcaaatta tccagctcat
     5941 caaattatct gtattcattt aaagcctatg gcgcaggcca aggtataaat gagcatagtt
     6001 attttatcaa aatcgatttc gatctgctca atgtcaagtt gactaatccc acttgtttta
     6061 ccgctatgct tagtggaact tctgtgactg gttctacggt aaaaatgggt gaatatagtg
     6121 cagaacaaat cagaaacggt gccacaccgg ttccttttga tatttcactt caaaactgcg
     6181 ttcgtgtgac taatattgag acaaaattag tttcaacaaa ggttggtact gaaaacgggc
     6241 aactccttgg taatactctc acaggtaatg acgcagcaaa aggagtcggt gtactcatag
     6301 aaggtttagc aactagtaaa aatcctctaa tgacattgaa acctaatgat tcaaattctg
     6361 tttataaaga ttacgacccg agaggcaaag acgatacaac aggaggagtt tacccggatc
     6421 aagatacagg tataacatac cctctccatt tccaggccac gctacaacag gatggaacta
     6481 taccaataga agctggtgag tttaaagcca ccagtacttt ccaggtaacc tacccttaat
     6541 aagtccaccg caccgccatc atctggcggt gcggtaattg ataaatcatt gcccccacca
     6601 atgtgactga taaaatgcca ggcaacacga catcatttat caggacacgt tatgaaaccg
     6661 accaccattg cttcacttca gaaatgtaag caggataaaa agcgtttcgc gaccatcacc
     6721 gcttacgact acagcttcgc gaaactattt gctgatgaag gacttaacgt catgctggta
     6781 ggcgattcgc tgggcatgac ggttcagggc cacgactcca cccttcccgt taccgtcgag
     6841 gatatcgcct atcataccgc cgccgtacgt cgcggcgcgc caaactgcct gctgttggct
     6901 gacctgccgt ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt
     6961 atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta
     7021 aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact taggtttaac cccacagtca
     7081 gtgaatattt tcggtggcta caaagttcag gggcgcggcg atgaagcggg cgatcaactg
     7141 ctcagcgatg cattagcctt agaagccgct ggggcacagc tgctggtgct ggaatgcgtg
     7201 ccggttgaac tggcaaaacg tattaccgaa gcactggcga tcccggttat tggcattggc
     7261 gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg acgccttcgg cattaccggc
     7321 ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct
     7381 gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc
     7441 cattaaggag tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc
     7501 gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg
     7561 atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta
     7621 ttttcgttaa cccgatgcag ttcgaccgcc cggaagatct ggcacgttat ccacggacct
     7681 tgcaggagga ctgcgagaag ctaaacaaac gtaaagtgga tttagttttc gccccttcgg
     7741 taaaagagat ctacccgaac ggtactgaaa cccatactta cgttgacgtt cccgggcttt
     7801 cgaccatgct ggaaggcgcg agccgtccgg ggcattttcg cggcgtttcg actatcgtca
     7861 gcaagctgtt taacctggtc cagccggaca tcgcctgctt cggtgaaaag gactttcagc
     7921 aactggcgct gatccgcaaa atggttgccg atatgggctt cgatattgag attgtcggtg
     7981 tgccaattat gcgcgccaaa gatggccttg cgctcagttc ccgtaatggt tatctgacgg
     8041 cggaacaacg caaaattgcg cccggtctgt acaaagtttt aagttcaatt gccgacaaat
     8101 tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat tgcggggcaa gaactgaatg
     8161 aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc cgacacattg ctggaagttt
     8221 ctgaaaccag caaacgggca gtaattctgg tagccgcctg gcttggcgat gctcgcctga
     8281 tcgacaacaa aatggtcgag ctggcgtaat acttaactgg cgctacggct gatggcgcca
     8341 gttattaatt taccccacgt catctgtcat aacacaggta acttaaataa ggttaattac
     8401 cttatcaatc tcagcaagag ataaatttgt catctctgca acgtctttcc gagacattcc
     8461 tttacttaga agacgcaggg cgaattcttg cctttcctcc tgtcttccct gctgaatccc
     8521 cttctcaatc cctttctctt caaaccactg cgccagcgtc atcatagact cccctcccgt
     8581 ttccctgtct ctcagcacac cataaaacaa atccgcttgt tcagtatgac cgcgttgcag
     8641 catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt caatcagcgt
     8701 gaccagttgc tccagcaata acattaagtc gcgctggcga atatgttttt gcagtagttc
     8761 gagaatcgca atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg tgatatccac
     8821 cagcgggaaa ggactgttat agacgcgtcg tgccagctcc ggcgagtaaa atatatcaaa
     8881 ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagta tcggcaccac
     8941 cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcagcaa tagaataacg
     9001 catcatgcga aaggccattt tcttatccgg cttgctttgg tgttcaatca caacatgcag
     9061 ataaccggga ttgccctgca tttgcacgga atagagcacg tccgtgctgt gtcctttcag
     9121 gctctcttca atgaaactcc ccgactctaa atgaagcgtg ttgaggtcac aaagttctcg
     9181 taattccact ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcat gcattaaaaa
     9241 ttgtttaaat accgcgtcat gcggtgtggt actcggtgca tccatcttcc ctgtcttccg
     9301 tgttgtcttg cgtgaaggca gtatccagtc aaggcgcgcc gtcgtcagcc gcctctttag
     9361 tgtttaccga gcagcgttca gagaaatttc aattcgttac aggcgataca tggcacgctt
     9421 cggcgcggta cgcaaactgc tgacgcaaat gccatcagat gagtagacac tcatcaaaaa
     9481 tcgggcaata ctgcgtgaga atttcctcaa agcaggcacc gcctgcttcg ttaacgacag
     9541 ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca ccgcgtgaaa
     9601 gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgaccagga ttttcttgac
     9661 gcagccggta ttctcgaaaa cgaagccatc gatatctgga atgtcaccaa cggcaagcgt
     9721 ttctccactt atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt taacggtgcg
     9781 gcggcccact gcgccagcgt cggcgatatt gtcatcatcg ccagcttcgt taccatgcca
     9841 gatgaagaag ctcgcacctg gcgacccaat gtcgcctatt ttgaaggcga caatgaaatg
     9901 aaacgtaccg cgaaagcgat tccggtacag gttgcttgat tgttatccct gcggctggtt
     9961 actcaccagc cgcgacatcg tctccagcga atccgttctt aagatataaa gtcgttttag
    10021 taacaacgga ttatcccccg gttttacttt gcctttcatg gttgtcaccg ccaggtgaaa
    10081 tccggcatcg tttgctgcct tcacggcttt gtcattaaat ccgccaaacg gatacgaaag
    10141 ataccagaca tgcggattaa attgcgccag agcgcggcgt gaacgtgcaa aatcaaacag
    10201 aatattgtgc tcactacggc tcagtaatat gggtcggcga tagccatcca cccgatgcaa
    10261 aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt tcgttaagct cagaaacgct
    10321 cataaattgc agcgattttg ggttccactt ctgcgggtga cgtttgatgc gcgaggtaac
    10381 aacaaacgcc gtcgccttca tgccatattg tttcaataca ggatacgcat agcggctcac
    10441 cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat tgatcttatt
    10501 cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt cgcgcagcca
    10561 ggccatttgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc gaaaacgggt
    10621 gttttcttca tcgcgcagaa tatggtgata ggttagcacc ggcaggccat tatcgggttg
    10681 ggcatccagc gcgctgatat aggcgagtcg atcgccaata cggatctgat accaggtttg
    10741 atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg ccagtacccc
    10801 aaatggcgca ctgcccgcac tcggcgcgtt atagaccggc gtatctttcc aggtaattaa
    10861 gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt tttgtcgccc
    10921 ctgaactggc tcgagatgcc ctttatcgat aaacccttta ccaaagccaa aattaaatgc
    10981 gtaataactt gcggcagtgg gctccacggc aatgatttgc ccggcccgaa tattccccac
    11041 ggtcaccatc ttgtcgccaa tttgcgccca gatcgcagca ttttcgatgg tttgcatata
    11101 acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga gaataacagc
    11161 ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta gcaaaagcgc
    11221 tataagaatc gtgggttaat actcatacaa attatgtttg aggacgaaag gcggattttt
    11281 ttgttgttgg tgccaaagac tactcacctc cggcccaccc agttcgacaa tacgctcgcg
    11341 aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta acggtaacgt
    11401 gacaccagaa atcacttcgc aacgggagtg tttgtggctt aataatgaag ccacgcggta
    11461 cggtggcgct ccggctatat cggttaagaa gatgacaccc tcaccagagt ccgcttcatg
    11521 cagagcatcg cacatcatgc ggctgagcat attagagctt aacccacgcc agaaattcac
    11581 ggcccggcac tgaagaagcg ccccatgttt tttctccagc gcatccagta tctcttgcgc
    11641 ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta gccgaaaatc
    11701 agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc tcccccaaat
    11761 ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat cagcgaccaa
    11821 cagatcaaat aaaacgccac aataaagacc accagtacgc caaaagtagt gaccagcgga
    11881 acatcattga taccgaggaa gccgtagcgg aaaccactga tcatataaac gattgggttc
    11941 aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gtgagtaaaa gaccccaccc
    12001 aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg
    12061 gcaaacacac cgttcagcaa acccgcaagg gagaacaaca ccgccgtgag caccagggtt
    12121 aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga aattgccgtc
    12181 accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc ggcaataatg
    12241 acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa cttggcacca
    12301 aaaaatgatg acgcaacgtt ggcgtaggca ttggtgatca ccgacatcat gatcagcccc
    12361 ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga accaatcagg
    12421 ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag cgtctgcacc
    12481 cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc cacccagtaa
    12541 agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac agctcttcca
    12601 gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt aactgcgtaa
    12661 atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca
    12721 gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga tcgagaataa
    12781 aggtttccga tttcagcttc gccagcagcg ccttcatcga ggtattttcc accagctcac
    12841 cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg tagtgtgtgg
    12901 tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac attgagcggc
    12961 gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta ggttcatgca
    13021 ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata cgcgcacgtt
    13081 cgttgcgttt tccccataga tcgagttgtt taagatattt ttcgctgcgg atgtacgcct
    13141 ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc acggtttcaa
    13201 acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg ttcacgacat
    13261 ctttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt accagagagc
    13321 tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc gcataaaaat
    13381 cacccgcttc gacctgcaaa tctattccac gaagcgcctg aacgccgcct ggataggttt
    13441 ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct tacgttctta
    13501 cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa cgtaattatt
    13561 tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca ataatgcact
    13621 atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg cacaagcgca
    13681 aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag aacgtttaac
    13741 cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg tcattcacac
    13801 cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactagaag ttgaacacat
    13861 tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa acccggaact
    13921 ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac atagctcatt
    13981 gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga acgtcatgga
    14041 acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac gcgggcagaa
    14101 agttaccatt cacggctggg cctatggcat tcacgacggc ttgctgcgtg atctggatgt
    14161 cacagcgact aaccgcgaaa cccttgagca acgttaccgt cacgggattt ccaacctcaa
    14221 gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat ccggcaactt
    14281 cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg gtaacgctgt
    14341 gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc gataaattca
    14401 accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat cgccaacgac
    14461 ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc cgagtcgatg
    14521 atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc tttgaggatt
    14581 ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat aaagtcgact
    14641 tcatgagata cctgaacttc acggcacaga tccgccataa acataaatga gccacgcagc
    14701 agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat ctgacgaccc
    14761 agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac agtatgtttc
    14821 atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc
    14881 aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa tctgttaata
    14941 aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg ctactaaaat
    15001 attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc gcgacgatta
    15061 ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact cattggcgga
    15121 ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggtct tgtgatgctc
    15181 ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctgta cgcagccctg
    15241 ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg ggcgctgact
    15301 ccgcgcagcg acattctggt cttcttcggc atctggctga tcctgccgtt tgtctggcgt
    15361 cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact gctgattagc
    15421 ggtggtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg caccttaagc
    15481 gccaatgcca cacctgcgga agctatctca cccgttgccg atcaggactg gcctgcctat
    15541 ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc cgataacgtc
    15601 cataatctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc gaacgatccg
    15661 ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta cctgtgtacc
    15721 gctcaccagc gcctgtttgc gctcgatgcc gccagcggca aagagaaatg gcattacgat
    15781 cctgagctga aaactaacga gtctttccag cacgtaacct gccgtggcgt ctcttatcat
    15841 gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt
    15901 cttccggtca atgatggtcg actgattgcg attaacgctg aaaacggcaa actgtgcgaa
    15961 accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac caaaccgggt
    16021 ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat ggccggttca
    16081 gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac
    16141 accggggagc tgctgtgggc ttttgatccc ggcgcgaaag atccgaacgc aatcccgtct
    16201 gacgaacaca cctttacctt taactcgcca aactcctggg caccagcggc ctatgacgcg
    16261 aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg gggcggtaac
    16321 cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc cactaccggg
    16381 aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga tcttccggca
    16441 cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat ttacgctccg
    16501 gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg gcgaactggt ggttccggca
    16561 ccggaaaaac cggttcccca aggtgcagcg aaaggcgatt acgtaacccc aactcaaccg
    16621 ttttctgaac ttagcttccg tccgacgaaa gatttgagcg gtgcggatat gtggggagcc
    16681 accatgtttg accagctggt gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt
    16741 ttcaccccgc catctgaaca gggtacgctg gtcttcccgg gtaacctggg gatgttcgaa
    16801 tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc aatggcactg
    16861 ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc cgatggagca gccgaaagat
    16921 gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc gtatggtgtc
    16981 acgctcaacc cgttcctctc accgtttggt ctgccgtgta aacagccagc atggggttat
    17041 atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg
    17101 caagacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg tatgccgatg
    17161 ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgctgctac ggcagataac
    17221 tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggccg tttaccagcg
    17281 ggtggtcagg cgacgccaat gacctatgaa gtgaatggca agcagtatgt ggtgatctcc
    17341 gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc ttatgcgctg
    17401 ccggatgatg tgaagtaacc ataatgcccg gagaaatccg ggcatatttc cgaatacggt
    17461 ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt
    17521 gcgccatata agcatgttct ttcggtgcgt cgtgattaaa cttcaccagc acttcactga
    17581 cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc
    17641 cattttctga caagatacgg aactgggtgc cgtgaatatg gaacggatgc agcatcatgt
    17701 cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct
    17761 tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc
    17821 cgtggttcat atgattcata ttaccgtgcc ccatatggcc catcatctgg ctgtgatcca
    17881 ttccagccat cgcctgatcg ccatatttct ccattagcat ctgcataccc atcatatcga
    17941 gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg
    18001 caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa
    18061 tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg
    18121 gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct
    18181 cgcccatcag caccggcagt tcgctcacct tcactggttc aggtagcaga ccaccgtcgc
    18241 tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac
    18301 agccattgag caaacgcagg cgcagccaac cacgcggggc agcgtgttgc gggtagattg
    18361 caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt
    18421 gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc ggaacatcat
    18481 cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca attaccacca
    18541 gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt ggatggaacc
    18601 agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
    18661 taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg
    18721 tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt
    18781 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
    18841 aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
    18901 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc cacagcggca
    18961 aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta
    19021 tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac
    19081 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
    19141 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
    19201 agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga
    19261 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
    19321 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
    19381 gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag acagctaccg
    19441 cgatctcagc ggcattggca ttcctgtcgc taaaaaatgc aaagccctgg cccgcgattc
    19501 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
    19561 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
    19621 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
    19681 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat
    19741 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
    19801 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
    19861 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
    19921 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtatcgtt
    19981 ctgccgtcag tatctaccca accataacgc cggtagctac gacgatccgc gctttaagct
    20041 ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
    20101 ctccgactgc accgatccta tcggtcccgg cgaaagcctt ttcacttcgg cattttatga
    20161 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
    20221 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
    20281 tggcttttat caggcggcga ttccgaccta ttacggcggt atcatgactt ttgcatgggc
    20341 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
    20401 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
    20461 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
    20521 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
    20581 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
    20641 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
    20701 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
    20761 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
    20821 agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag
    20881 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
    20941 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
    21001 cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga
    21061 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
    21121 ggtggatgtg aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
    21181 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
    21241 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
    21301 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
    21361 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
    21421 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
    21481 ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg tgtattcatg ccctgcccgt
    21541 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
    21601 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
    21661 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
    21721 tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat cgcagcctta
    21781 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
    21841 caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattacac cgcagtctgg
    21901 aaaatcaccc catcggcttt ttcggtgtac tgagaaagct ggttgaagtt cagataacga
    21961 taagtatcaa cggcagtttt atctacttgc gcaacgtagg tctggtactc ttccggcgtc
    22021 ggcagtttgc caatcagcgc cgcaacagcc gccagttctg cagaagccag gaagacattc
    22081 gcgccagtcc ccagacggtt cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg
    22141 tctgctacac gcgcctggtt acccatacac agagaacagc ccgggatctc gatacgagcc
    22201 ccgctcttac cgaagacgct gtagtagcct tcttcagtca actgtgcggc gtccatacgg
    22261 gttggcggtg ccacccacag gcgggtcggc aactggcctt tatgcgcatc cagcagttta
    22321 cccgcagcac ggaagtgacc gatgttagtc atgcaagaac cgataaatac ttcgtcgatc
    22381 ttctcgccct gtaccgcaga cagcggacgc gcgtcatccg ggtcgttcgg agcacacagg
    22441 attggctctt taatatccgc cagatcgatg tcaatcactg ccgcgtattc cgcatctgca
    22501 tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat acgacgttcg
    22561 agggtacgac gatcgccata accttccgcg atcatccact tcagcaggac gatgttagag
    22621 ttcaggtatt cgatgatcgg ttctttgttc agcttgatag tacaaccagc ggcagaacgc
    22681 tcagcggacg catcggtcag ttcaaatgcc tgctcaactt tcagatccgg cagaccttca
    22741 atttccagaa tgcgaccaga gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga
    22801 ccttgtttga tcgcatacag cgggatcgcg tgtaccagat cgcgcagggt gatgcccggc
    22861 tgcattttgc ctttgaagcg caccagaacg gattccggca tatccagcgg cattacgcct
    22921 gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc gatcgggaaa
    22981 cgggtatggg agtcacctcc ggtaccgacg gtatccggca gcagcatacg gttcagccag
    23041 gagtggatta cgccgtcacc cggacgcagc gacacaccgc cacggttcat aatgaagtcc
    23101 ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt gtggcagaaa
    23161 gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag ttcatcacgg
    23221 gtcatcgggc cggtggtgtc ctgagagcct acagaagtca ttttcggttc gcagtacgcg
    23281 cccggacgaa tgcctttcac gccacaggca cggcctacca ttttctgcgc cagcgagaag
    23341 ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactgtg cggcagacca
    23401 agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcgggat acggccgcca
    23461 gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgcct
    23521 gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caatcacgtc gcccatgttc
    23581 aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt gttaaagaag
    23641 atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga
    23701 atatcatcgc ccataaacca cagaacggag ttagtggcgg atttacgcga agaacccgta
    23761 cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc ttcgatttgc
    23821 ttgatcggac caacaacacc aggctggtct ggctcaatac cttcacgggc gtttttcagc
    23881 atcgccagcg cgtgcagtgg gatatccggg cgtgaccacg catccggtgc cggagaaagg
    23941 tcatcggtgt tagtttcgcc agtgactttg aagacggtaa cggtcagttt ttcagccagc
    24001 gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcattacctg cttcgcatat
    24061 tcgttgcctg ctttcgcttt ctcttctacg tcatagaagt tatcgaacat cagcagtgtg
    24121 tgagaaagtg ctttggcagc gatcggtgcc agtttggcat catccagcgc gtcgatcagc
    24181 ggatgaatgt tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc
    24241 agcagagggg atttggcttc gcctttcgcg atagcagcca ggaagcctgc tttgacatag
    24301 gcggcttcat cgacgccagg gggaacacgg ttggttaaca gatctaacag gaattcttct
    24361 tcgcccgcgg gcgggttttt cagcagctct acaagtgcgg ccatttggtt tgcatccagg
    24421 ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc
    24481 acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg
    24541 tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt
    24601 gacgatgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga
    24661 tggtctagag attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct
    24721 ttctcaacga atcccaagat tagtcaaaat ttaaactacc gcctctttat actcggattc
    24781 acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga
    24841 aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg
    24901 ccgcctctac cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat
    24961 cgacacaaca gactgcgcct gacatcaccg cagttacgct tcctgcaacg gtttccgcac
    25021 cgcccgtaac gcccgccgtc gtcaaatccg cattcagcac tgcacaaatc gatcaatggg
    25081 ttgcgcctgt cgcgctgtac cccgattctc tgctttcaca agtgttaatg gcatcaacct
    25141 atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg
    25201 atgctgctat tcaggcagta tctgaccagc cgtgggacgc cagcgttaaa tcactggtgg
    25261 cctttccaca attgatggca ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg
    25321 atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg cggcaactgg
    25381 cgcaacaaac cggctcgctg aagtcatcaa ccgaacagaa agttattacc acaacgaaga
    25441 aagctgtacc ggtaacacag acagtcacgg ctcccgtcat accatccaat accgtttcaa
    25501 ctgccagccc catcattaca gagcctgcaa caaccgtcat ttccattgag cccgccaatc
    25561 ctgatgtggt ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata
    25621 ctgcgtatcc gccggtttat ctgccaccac cagccggaga accgtttatt gacagctttg
    25681 tgcgcggatt cggctatagc atgggtgttg ctaccacgta cgcactattc agcagcatcg
    25741 actgggacga cgacgatcat gaccatcatc atcatgacga tgatgattat catcaccacg
    25801 atggcggtca tcgtgacggt aatggctggc aacacaacgg cgacaacatc aatatcgacg
    25861 tcaacaattt caaccgtatc accggtgagc atcttactga taagaatatg gcatggcggc
    25921 acaatccaaa ctaccgtaat ggtgtgccct atcatgatca ggatatggca aagcggtttc
    25981 atcaaaccga tgtcaacggc ggaatgagtg ccacgcagct acctgctcca acacgcgaca
    26041 gccagcgtca ggcggcagca agtcagtttc agcaacgaac acacgccgcc cccgtcatta
    26101 cacgagatac ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa cactatggga
    26161 gctatgacga cttccgcgac ttcagccgtc gccaacccct gacccagcaa caaaaggacg
    26221 ccgctcgtca gcgttatcag tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa
    26281 tgcagactaa cccacagaac cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg
    26341 caactcccga acagcgccag gtatttaagg aaaaagtaca gcagcgccca ctgaaccaac
    26401 agcaacgtga taacgcccgc cagcgtatac aatcggcatc acctgaacaa cgtcaggttt
    26461 ttcgggaaaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    26521 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
    26581 gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    26641 ccggatgttc cggcaaacga acaattactt cttcttcgct ttcgggttcg gcaggtcggt
    26701 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    26761 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    26821 acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac ggtgagattc
    26881 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
    26941 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    27001 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    27061 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    27121 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    27181 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tccacttcca cgccagcttt
    27241 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    27301 acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    27361 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    27421 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    27481 cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc
    27541 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    27601 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    27661 aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt
    27721 gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    27781 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    27841 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    27901 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    27961 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    28021 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    28081 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    28141 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    28201 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    28261 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    28321 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    28381 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    28441 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    28501 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    28561 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    28621 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    28681 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    28741 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    28801 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    28861 cgacaacgaa ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    28921 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    28981 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgagtaa catgcgggat
    29041 catcacccag ttacggctca ggttcgcacc agaaattttc tggatacggc ccagttccac
    29101 ttcttcgatt tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcctgggat
    29161 accgccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    29221 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    29281 aacgccaaac tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt
    29341 ttctgcaaat tcagatttgc cttccgcttt cgcagccggt gctgctgccg gagcttcagc
    29401 ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc
    29461 gccttcaact tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac
    29521 tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag ctttgtcgcc
    29581 ttctacggta atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc
    29641 ggtcacttca acttcgtcac cgccgatatc cggaacgtta acttctttca cgccagccgc
    29701 tggtgcaggg gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc
    29761 ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt
    29821 cactttgatc tctttcacgg tgccagcaaa cggagccgga acttccatag acgctttatc
    29881 gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat
    29941 ttcggtcact tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc
    30001 agccgctggt gctgctgccg gagctgcttc tttcttctct tctgcctgag caggtgcagc
    30061 gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc
    30121 gccaacagag actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga
    30181 ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt
    30241 gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat
    30301 agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg
    30361 aatttggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg
    30421 cccagcgccg ctacaaccac gtaggaagca tcaacttcga agtggtgacg caggttctca
    30481 cggctgtcgg aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg
    30541 taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg
    30601 tcgttcatca cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc
    30661 cagcgttcac aatcctgacc atctcgcgcc agttcggtga aggaggtcac gctataaacg
    30721 tcagaaccta cgccgtaatc tttcgccagg atctctgccg cttcacggac gtgacgcagg
    30781 atggaaccgg agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg
    30841 tagatacctt tacggatacc ttcctcagca ccttccggca ttgccggcat gtggtagttt
    30901 tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt tttcaccgta catacgcttc
    30961 agaccgtcat gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag
    31021 ttcgggatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcaccg
    31081 ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca
    31141 gccgcccagc acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaac
    31201 ggaatcatcg gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct
    31261 gcgcccaact cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa
    31321 gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc
    31381 tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga
    31441 cgatctttga tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg
    31501 gtagagatct ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc
    31561 tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc
    31621 tgtgcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcagtttt
    31681 tcgatatctg catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc
    31741 atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg
    31801 taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc ctgcgctttc
    31861 ttgaatgcag cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgc
    31921 tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat atttaccgaa gaagtgttca
    31981 cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc
    32041 atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc
    32101 cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg
    32161 atcttgccgt taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaag
    32221 accaggttat ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg
    32281 tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca
    32341 cggtgttcca gatatttcag gaatttagcc tggtaaatag caccaatcgg ccccagaccc
    32401 atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag
    32461 aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct
    32521 tccaggaaag cacgagcgta cacgcccggg gagatgtggc cctggaagta aaccaggtcg
    32581 ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt
    32641 gcggaagact ggaaggacgc catatggccg cccagttcga ggtctttttt cgacgcacgc
    32701 agcaccgtca tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga
    32761 ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgatacct
    32821 gtgcctgcgg ctacgtttac accgcctttg cgggcttcag caagcagttg gtcgatcaga
    32881 tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg
    32941 cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct
    33001 atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat
    33061 aagacaggtt ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct
    33121 ggataatttt taccagaaaa atcactaatt ctttcgttgc tccagacgac gcagagaacg
    33181 ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt tcttcgataa aggccagatg
    33241 gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg
    33301 ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt
    33361 ctggcggaca ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac
    33421 attgtgggcc gcttcggtga cggcaatctg atactggagt acggcgtttg attccgcgtc
    33481 cagatcgcca gactgctgcg ccagctctat ggcgtggtgg agttcacgga tgcgttcctt
    33541 gtcttcatcg gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg
    33601 tgtttcgagc aagtcatact gtgactcagg atggtcggag agcagctcca ccagcggatc
    33661 gctgaagctt tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa
    33721 caagcccttc gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa
    33781 ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag
    33841 gatcaaaaac tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct
    33901 gtaggccatg agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca
    33961 tgtctgtgac gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa
    34021 tcatgaaact gtgcacattt taacaacttg
//
LOCUS       DAMZII010000039        33293 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZII010000039 DAMZII010000000
VERSION     DAMZII010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.039"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            94..1047
                     /locus_tag="POA77_001852"
     CDS             94..1047
                     /locus_tag="POA77_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6365298.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(1122..1208)
                     /locus_tag="POA77_001853"
     tRNA            complement(1122..1208)
                     /locus_tag="POA77_001853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1172..1174),aa:Leu,seq:gag)
     gene            complement(1223..1555)
                     /gene="secG"
                     /locus_tag="POA77_001854"
     CDS             complement(1223..1555)
                     /gene="secG"
                     /locus_tag="POA77_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI6365299.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1783..3120)
                     /gene="glmM"
                     /locus_tag="POA77_001855"
     CDS             complement(1783..3120)
                     /gene="glmM"
                     /locus_tag="POA77_001855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI6365300.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(3113..3961)
                     /gene="folP"
                     /locus_tag="POA77_001856"
     CDS             complement(3113..3961)
                     /gene="folP"
                     /locus_tag="POA77_001856"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI6365301.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(4051..5985)
                     /gene="ftsH"
                     /locus_tag="POA77_001857"
     CDS             complement(4051..5985)
                     /gene="ftsH"
                     /locus_tag="POA77_001857"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI6365302.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(6085..6714)
                     /gene="rlmE"
                     /locus_tag="POA77_001858"
     CDS             complement(6085..6714)
                     /gene="rlmE"
                     /locus_tag="POA77_001858"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDI6365303.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6840..7133
                     /gene="yhbY"
                     /locus_tag="POA77_001859"
     CDS             6840..7133
                     /gene="yhbY"
                     /locus_tag="POA77_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI6365304.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7289..7765)
                     /gene="greA"
                     /locus_tag="POA77_001860"
     CDS             complement(7289..7765)
                     /gene="greA"
                     /locus_tag="POA77_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI6365305.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            8013..9446
                     /gene="dacB"
                     /locus_tag="POA77_001861"
     CDS             8013..9446
                     /gene="dacB"
                     /locus_tag="POA77_001861"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI6365306.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9486..10658)
                     /gene="cgtA"
                     /locus_tag="POA77_001862"
     CDS             complement(9486..10658)
                     /gene="cgtA"
                     /locus_tag="POA77_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDI6365307.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10674..11639)
                     /locus_tag="POA77_001863"
     CDS             complement(10674..11639)
                     /locus_tag="POA77_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6365308.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11766..12023)
                     /gene="rpmA"
                     /locus_tag="POA77_001864"
     CDS             complement(11766..12023)
                     /gene="rpmA"
                     /locus_tag="POA77_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI6365309.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12044..12355)
                     /gene="rplU"
                     /locus_tag="POA77_001865"
     CDS             complement(12044..12355)
                     /gene="rplU"
                     /locus_tag="POA77_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI6365310.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12614..13585
                     /gene="ispB"
                     /locus_tag="POA77_001866"
     CDS             12614..13585
                     /gene="ispB"
                     /locus_tag="POA77_001866"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDI6365311.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13813..14091
                     /gene="sfsB"
                     /locus_tag="POA77_001867"
     CDS             13813..14091
                     /gene="sfsB"
                     /locus_tag="POA77_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDI6365312.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14139..15398)
                     /gene="murA"
                     /locus_tag="POA77_001868"
     CDS             complement(14139..15398)
                     /gene="murA"
                     /locus_tag="POA77_001868"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6365313.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15453..15707)
                     /gene="ibaG"
                     /locus_tag="POA77_001869"
     CDS             complement(15453..15707)
                     /gene="ibaG"
                     /locus_tag="POA77_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDI6365314.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15867..16160)
                     /gene="mlaB"
                     /locus_tag="POA77_001870"
     CDS             complement(15867..16160)
                     /gene="mlaB"
                     /locus_tag="POA77_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDI6365315.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16160..16795)
                     /gene="mlaC"
                     /locus_tag="POA77_001871"
     CDS             complement(16160..16795)
                     /gene="mlaC"
                     /locus_tag="POA77_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDI6365316.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16814..17365)
                     /gene="mlaD"
                     /locus_tag="POA77_001872"
     CDS             complement(16814..17365)
                     /gene="mlaD"
                     /locus_tag="POA77_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDI6365317.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17370..18152)
                     /gene="mlaE"
                     /locus_tag="POA77_001873"
     CDS             complement(17370..18152)
                     /gene="mlaE"
                     /locus_tag="POA77_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDI6365318.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18160..18969)
                     /gene="mlaF"
                     /locus_tag="POA77_001874"
     CDS             complement(18160..18969)
                     /gene="mlaF"
                     /locus_tag="POA77_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDI6365319.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19179..20156
                     /locus_tag="POA77_001875"
     CDS             19179..20156
                     /locus_tag="POA77_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDI6365320.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20170..21156
                     /gene="kdsD"
                     /locus_tag="POA77_001876"
     CDS             20170..21156
                     /gene="kdsD"
                     /locus_tag="POA77_001876"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDI6365321.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21177..21743
                     /gene="kdsC"
                     /locus_tag="POA77_001877"
     CDS             21177..21743
                     /gene="kdsC"
                     /locus_tag="POA77_001877"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDI6365322.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21740..22315
                     /gene="lptC"
                     /locus_tag="POA77_001878"
     CDS             21740..22315
                     /gene="lptC"
                     /locus_tag="POA77_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDI6365323.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22284..22841
                     /gene="lptA"
                     /locus_tag="POA77_001879"
     CDS             22284..22841
                     /gene="lptA"
                     /locus_tag="POA77_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDI6365324.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22848..23573
                     /gene="lptB"
                     /locus_tag="POA77_001880"
     CDS             22848..23573
                     /gene="lptB"
                     /locus_tag="POA77_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDI6365325.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23621..25054
                     /gene="rpoN"
                     /locus_tag="POA77_001881"
     CDS             23621..25054
                     /gene="rpoN"
                     /locus_tag="POA77_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDI6365326.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25077..25364
                     /gene="hpf"
                     /locus_tag="POA77_001882"
     CDS             25077..25364
                     /gene="hpf"
                     /locus_tag="POA77_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDI6365327.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25482..25973
                     /gene="ptsN"
                     /locus_tag="POA77_001883"
     CDS             25482..25973
                     /gene="ptsN"
                     /locus_tag="POA77_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDI6365328.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26019..26873
                     /gene="rapZ"
                     /locus_tag="POA77_001884"
     CDS             26019..26873
                     /gene="rapZ"
                     /locus_tag="POA77_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI6365329.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26870..27142
                     /gene="npr"
                     /locus_tag="POA77_001885"
     CDS             26870..27142
                     /gene="npr"
                     /locus_tag="POA77_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDI6365330.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27356..27988
                     /gene="yrbL"
                     /locus_tag="POA77_001886"
     CDS             27356..27988
                     /gene="yrbL"
                     /locus_tag="POA77_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDI6365331.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27985..28713)
                     /gene="mtgA"
                     /locus_tag="POA77_001887"
     CDS             complement(27985..28713)
                     /gene="mtgA"
                     /locus_tag="POA77_001887"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDI6365332.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28710..29363)
                     /gene="elbB"
                     /locus_tag="POA77_001888"
     CDS             complement(28710..29363)
                     /gene="elbB"
                     /locus_tag="POA77_001888"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDI6365333.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29593..31929)
                     /gene="arcB"
                     /locus_tag="POA77_001889"
     CDS             complement(29593..31929)
                     /gene="arcB"
                     /locus_tag="POA77_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDI6365334.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32025..32954)
                     /locus_tag="POA77_001890"
     CDS             complement(32025..32954)
                     /locus_tag="POA77_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI6365335.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT         8332 a         8930 c         8006 g         8025 t
ORIGIN      
        1 tcggaattaa agtaaaagtt aaatgaaata aagtaaattt cagcgcattc tatttttact
       61 ttatgttttt atgctcagta aaaggaatga ccgatgaatg gtgaatattt tgtacgcctc
      121 gcattacata ctctgaaatg cactcaaaaa gatttagcaa gccacttagg tgtttcctca
      181 acacaaatta gcaagtggaa aaaaggggaa cacatgtccg ctgatatgga aaaaaagttc
      241 agaaatatta cacaaatagg agattattct cctcaactag ttgaatggac gggctcagtc
      301 gcgaatgctg agaaatggga tcgacttatc cattttcttg ctcgacgagc tatggacgat
      361 gctgaaacgg gttatataac acaacttctt actgacgaag atggtttttt ggttgaagaa
      421 acaattgatg tattaaagcg aataggtttt cctacacctc tttcctttcc tgaaggactt
      481 aatattgacg acgataatgc tgatgaagaa gaggcttttt gggagatatt agagagcaat
      541 gctcattgtt ctgtaattaa tgatatttat catgctctga atgatgttta tggtttctac
      601 atagcatatg ttgatgagtt gattcaggac gacgatttag atgtctattc aagcgaagcc
      661 atcaatattc agagttcatt gatatcactt gctgcatgca aaattgaaat tgatactcct
      721 gttgcgtcaa actttaaaga atttcgttat agagttaaaa aagactacga aaattggttg
      781 aatcaactca agatgatggc attcagggcg ggaattccgt taagagccga acttctggaa
      841 atggtttata acaccgctga ccaacttagc gttgcagcag aagccgaacg tttcgatttc
      901 aataaatctc gcattcatcc agatatatac atgaacgaga tccttactgg catgagaata
      961 attcaccaag tgcttccggt aataatgcaa aagttagaaa ttacagattt caaactagat
     1021 gaaacagatt tatgcctggg taaatagtta tcaaattaag aatacttagc ctggcttata
     1081 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     1141 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     1201 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gctggagcag
     1261 ctggttgagt ttgttcggtt ttcaccggtg cactcagatt ttcccattcg ctacctttat
     1321 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     1381 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     1441 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     1501 gaccaacaag gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca
     1561 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     1621 atctttccca ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     1681 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     1741 aaaggcgaca atcgccgcct ttttagccag ttgtctaacg ctttaaacgg cttttactgc
     1801 atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac
     1861 gcgaattaac ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc
     1921 agcttcaacc tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc
     1981 tgcggtgtaa cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     2041 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     2101 gtcaccggta gtagttttat ccagtaggat cacatgaccg gaattctctg caccgatacg
     2161 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     2221 tggaattcct aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac
     2281 agcgccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     2341 atcgccatcg actttattgc cttcatggtc gaccataatc acgcgatcgc catcgccgtc
     2401 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     2461 ggtagccccc acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac
     2521 gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca
     2581 atccaccaca atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     2641 ctcgatatag cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc
     2701 aacgcagctg atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag
     2761 tttggtgccg tcgatagaga agaatttaat cccattatcg tagaacgggt tatgcgatgc
     2821 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     2881 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     2941 tgactccagc atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc
     3001 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     3061 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     3121 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     3181 ctttgacgtc atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg
     3241 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     3301 ttcgtgacat acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     3361 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     3421 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     3481 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     3541 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     3601 aaagggagcg gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt
     3661 ctggtttgga tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     3721 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     3781 actcgccacc aacgtcaatg atcgtcgcgc cagcattgat catcagattc gcatgtttca
     3841 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     3901 ggatccccat tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca
     3961 tgatgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     4021 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     4081 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     4141 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     4201 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     4261 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     4321 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     4381 tttcgctttc gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag
     4441 tggacccaat ttctcagaga agccccactg ggtcaccatg ttacgtgcca gattggtcgc
     4501 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccataga tgatctcttc
     4561 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     4621 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     4681 cgtcactttg tgcaccggat cgtgttcagg caccaggcga ccgataatcg catgacccgc
     4741 ttcgtggtaa gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     4801 acccatcatg attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     4861 gccacgagca gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa
     4921 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg gtacgcgacg
     4981 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccga ccacaacctg
     5041 acggtcgaaa cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc
     5101 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     5161 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     5221 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     5281 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     5341 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     5401 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     5461 gctcggctcg cgcagatact ctaccagttc agcaacttct tcttttgctt cgtcgcagcc
     5521 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     5581 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     5641 gacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     5701 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     5761 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     5821 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     5881 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     5941 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     6001 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     6061 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     6121 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     6181 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     6241 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     6301 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     6361 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     6421 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     6481 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     6541 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     6601 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     6661 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     6721 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     6781 tatatgggag atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga
     6841 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     6901 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     6961 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct
     7021 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     7081 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     7141 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     7201 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     7261 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     7321 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     7381 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     7441 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     7501 caaaaataac gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca
     7561 gcttggcttc gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt
     7621 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
     7681 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
     7741 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
     7801 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
     7861 aaaaaatacc gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat
     7921 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
     7981 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
     8041 tgaccagctg tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac
     8101 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
     8161 ttgattacca cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc
     8221 tggcggcgtt gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag
     8281 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
     8341 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
     8401 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
     8461 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg
     8521 accgcaactg tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta
     8581 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag
     8641 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
     8701 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
     8761 atggagccag ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct
     8821 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
     8881 aacagtcggc cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga
     8941 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc
     9001 gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
     9061 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
     9121 tgatgcaggt gttgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
     9181 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
     9241 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
     9301 tcattaccac agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag
     9361 tagaaccggc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
     9421 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt
     9481 gctgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca
     9541 tcccagtcct catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc
     9601 cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca
     9661 atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag
     9721 atcagataat atttatcttc ccagcccagc gcttcagcaa tcgctttcgc tttctcttca
     9781 gcttctgcct tatccagcag atcgatctta ttgaacacta accaacgtgg tttcgccgcc
     9841 agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg
     9901 gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc
     9961 ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata
    10021 tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga
    10081 gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc
    10141 gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc
    10201 agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact
    10261 gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc
    10321 tgaccgtgtt tggtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta
    10381 cctaccggca ctttaattgt gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg
    10441 ccattctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc
    10501 aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg
    10561 ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct
    10621 gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg
    10681 cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga
    10741 ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg
    10801 tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac
    10861 agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc
    10921 atcgcttccg ccagggcgcc atatcctacc aaggtattca gtccgcaaaa aattaaacat
    10981 gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca
    11041 attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa
    11101 accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact
    11161 ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc
    11221 accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc
    11281 aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa
    11341 gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg
    11401 gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc
    11461 aacctcttct tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa
    11521 aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc
    11581 ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata
    11641 ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt
    11701 tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga
    11761 aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat
    11821 ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg
    11881 ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg
    11941 ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag
    12001 ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag
    12061 taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac
    12121 gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga
    12181 ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca
    12241 gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg
    12301 tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa
    12361 actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag
    12421 ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa
    12481 aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcactcta
    12541 tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag
    12601 cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg
    12661 tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc cagctgatca atcagttagg
    12721 ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc
    12781 acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat
    12841 ccacacggcg actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa
    12901 agctaccgcc aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta
    12961 tacccgcgcg ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc
    13021 agaagccgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc
    13081 ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga
    13141 ggctgccgcg cagtgttccg ggattctggc tggctgtacg ccggaagagg agaaaggcct
    13201 gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta
    13261 caatgccgat ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg aaggtaaacc
    13321 gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg
    13381 taccgccatc gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa
    13441 cgcttgtgga tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat
    13501 cgcagcatta caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca
    13561 catcgctgtt caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg cgcgggatga
    13621 ttccagtaaa ttccataaaa acactaattc agcgctataa cacctgaaaa aaagggcgat
    13681 gaatctttat atattctgaa tattcacact ctttacagga actttttaga gcaataggcc
    13741 atcaggagta tagtgatgcc cgacagaaga agtgttctga atgaaagcga ctaacttaag
    13801 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    13861 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    13921 acgctggcaa acgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    13981 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    14041 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    14101 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    14161 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    14221 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    14281 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    14341 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    14401 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    14461 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    14521 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    14581 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    14641 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    14701 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    14761 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    14821 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    14881 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    14941 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    15001 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    15061 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    15121 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    15181 tcaatatgca cagaaccatt acgttctact ttcgccccca gctggcttag cagcttcatt
    15241 gatgtatcga cgtctttcag ttttgggacg ttctggatct ctaccggttc ttccgccagt
    15301 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    15361 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    15421 ttcatatccg ctaccggcta atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    15481 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    15541 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    15601 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    15661 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    15721 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    15781 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    15841 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    15901 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    15961 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    16021 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    16081 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    16141 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    16201 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    16261 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    16321 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    16381 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    16441 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    16501 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    16561 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    16621 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    16681 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    16741 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    16801 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    16861 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    16921 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    16981 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    17041 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    17101 gttgttcaat ttccagcgtt acgcgcggca gataggtttt cgggtccagc gtaatatccg
    17161 ccacccgacc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    17221 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    17281 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    17341 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    17401 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    17461 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    17521 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    17581 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    17641 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    17701 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    17761 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    17821 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    17881 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    17941 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    18001 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    18061 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    18121 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    18181 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    18241 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    18301 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    18361 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    18421 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    18481 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    18541 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    18601 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    18661 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    18721 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    18781 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    18841 cgatggcccc atgatcgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    18901 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    18961 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    19021 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    19081 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    19141 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    19201 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    19261 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    19321 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    19381 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    19441 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    19501 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    19561 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    19621 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    19681 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    19741 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    19801 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    19861 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    19921 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    19981 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    20041 tgctgtgctg gcggcgctcc ccacaaccgg gccgtggtgt aggggtatta ttaactggcg
    20101 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    20161 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    20221 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    20281 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    20341 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    20401 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    20461 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    20521 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    20581 gcgcgcgccg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    20641 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    20701 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    20761 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    20821 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    20881 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    20941 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    21001 acgccggggg gaatacgcgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    21061 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    21121 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    21181 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatggcaa
    21241 aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca gatggcctga
    21301 tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    21361 ttcgttgtgc gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    21421 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    21481 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    21541 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    21601 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    21661 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    21721 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    21781 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    21841 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    21901 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    21961 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    22021 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    22081 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    22141 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    22201 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    22261 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    22321 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    22381 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    22441 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    22501 tcgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    22561 caacgttcta ccagatgcag gacaacggta aacccgttga aggtcacgct tcccagatgc
    22621 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggtcg
    22681 atagcaacat taagggcgat aagatcactt acctggtgaa agagcagaaa atgcaggctt
    22741 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggataaaa
    22801 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    22861 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    22921 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    22981 ctttctatat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    23041 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    23101 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    23161 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    23221 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    23281 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    23341 ttgccggtgt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    23401 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    23461 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    23521 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    23581 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    23641 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    23701 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    23761 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    23821 ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    23881 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    23941 tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    24001 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    24061 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    24121 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    24181 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    24241 taaaaccacg ccgtggctgg aagaggccag attgatcatt agcgatcatc tcgatctgtt
    24301 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    24361 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    24421 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacagt
    24481 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    24541 caataacgct cgcaatgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    24601 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    24661 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    24721 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    24781 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    24841 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    24901 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    24961 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    25021 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    25081 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    25141 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    25201 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    25261 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    25321 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    25381 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    25441 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    25501 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    25561 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc
    25621 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    25681 atggtattgc aattccgcat ggcaaactgg aagaagatac tctgcgtgcc gttggcgttt
    25741 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac
    25801 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg
    25861 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    25921 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    25981 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    26041 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    26101 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaaccctg gccgatcgcg
    26161 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    26221 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    26281 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    26341 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    26401 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    26461 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac aatggtcttt gagtctttcg
    26521 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    26581 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataagccc gtcgccgcgt
    26641 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgagc
    26701 tatggttacc tatgctggaa accaacaacc gtagctacct gacggtcgcc attggttgta
    26761 ccggcgggaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    26821 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    26881 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    26941 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    27001 tgaagccaac agcgttattg cgctgctgat gttagattct gccaaaggac ggcagattga
    27061 agttgaagcg accggcccac aggaagagga agcactggca gccgttatcg ccctctttaa
    27121 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    27181 cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt
    27241 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agaggcattg tttaggtttt
    27301 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    27361 tcgcttatct gaacaaaatc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    27421 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    27481 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    27541 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    27601 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg agcaatgccg
    27661 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    27721 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    27781 tgagtcagag gttatcccgg tcgtctgcga caacattggt gaaagcacat tgattccact
    27841 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    27901 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    27961 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    28021 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    28081 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    28141 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    28201 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    28261 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    28321 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    28381 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    28441 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    28501 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    28561 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    28621 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    28681 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    28741 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    28801 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    28861 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    28921 gggaaatcga aaattttcgg cagcatcgct ggggcaatac acataaaacc aagcggtttt
    28981 ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    29041 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    29101 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    29161 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    29221 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    29281 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    29341 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    29401 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    29461 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    29521 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    29581 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    29641 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    29701 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    29761 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    29821 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    29881 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    29941 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    30001 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    30061 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    30121 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    30181 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    30241 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    30301 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    30361 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    30421 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    30481 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    30541 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    30601 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    30661 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    30721 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    30781 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    30841 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    30901 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    30961 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    31021 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    31081 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    31141 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    31201 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    31261 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    31321 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    31381 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    31441 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    31501 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    31561 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    31621 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    31681 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    31741 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    31801 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    31861 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    31921 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    31981 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    32041 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    32101 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    32161 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    32221 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    32281 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    32341 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    32401 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    32461 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    32521 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    32581 gcatccggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    32641 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    32701 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    32761 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    32821 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgtagcgtc
    32881 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    32941 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    33001 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    33061 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    33121 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    33181 caattaaggc agtataaaat gctggttttc tcgtcagttc aaggcaggat aagggttaac
    33241 acgcctttat gacagtcagg aattgactgt ttctctaacg acttcccttt tag
//
LOCUS       DAMZII010000040        32744 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZII010000040 DAMZII010000000
VERSION     DAMZII010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.040"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            7..591
                     /locus_tag="POA77_001891"
     CDS             7..591
                     /locus_tag="POA77_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6365336.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            671..1366
                     /locus_tag="POA77_001892"
     CDS             671..1366
                     /locus_tag="POA77_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6365337.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1396..3912
                     /locus_tag="POA77_001893"
     CDS             1396..3912
                     /locus_tag="POA77_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6365338.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3923..5014
                     /locus_tag="POA77_001894"
     CDS             3923..5014
                     /locus_tag="POA77_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6365339.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQTNDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFNITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5057..5917)
                     /gene="rsmI"
                     /locus_tag="POA77_001895"
     CDS             complement(5057..5917)
                     /gene="rsmI"
                     /locus_tag="POA77_001895"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI6365340.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5981..8017
                     /gene="lpoA"
                     /locus_tag="POA77_001896"
     CDS             5981..8017
                     /gene="lpoA"
                     /locus_tag="POA77_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDI6365341.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7975..8370
                     /locus_tag="POA77_001897"
     CDS             7975..8370
                     /locus_tag="POA77_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDI6365342.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8390..8980
                     /gene="diaA"
                     /locus_tag="POA77_001898"
     CDS             8390..8980
                     /gene="diaA"
                     /locus_tag="POA77_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDI6365343.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8990..9565
                     /gene="yraP"
                     /locus_tag="POA77_001899"
     CDS             8990..9565
                     /gene="yraP"
                     /locus_tag="POA77_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDI6365344.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9679..10719)
                     /locus_tag="POA77_001900"
     CDS             complement(9679..10719)
                     /locus_tag="POA77_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6365345.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10792..11427)
                     /locus_tag="POA77_001901"
     CDS             complement(10792..11427)
                     /locus_tag="POA77_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDI6365346.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11555..12073
                     /gene="yhbO"
                     /locus_tag="POA77_001902"
     CDS             11555..12073
                     /gene="yhbO"
                     /locus_tag="POA77_001902"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDI6365347.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12053..12496)
                     /locus_tag="POA77_001903"
     CDS             complement(12053..12496)
                     /locus_tag="POA77_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDI6365348.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12547..12849
                     /gene="yhbQ"
                     /locus_tag="POA77_001904"
     CDS             12547..12849
                     /gene="yhbQ"
                     /locus_tag="POA77_001904"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDI6365349.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12836..13339)
                     /locus_tag="POA77_001905"
     CDS             complement(12836..13339)
                     /locus_tag="POA77_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6365350.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13333..13857)
                     /locus_tag="POA77_001906"
     CDS             complement(13333..13857)
                     /locus_tag="POA77_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDI6365351.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14066..15061
                     /locus_tag="POA77_001907"
     CDS             14066..15061
                     /locus_tag="POA77_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6365352.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15070..15948
                     /locus_tag="POA77_001908"
     CDS             15070..15948
                     /locus_tag="POA77_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6365353.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            16154..17161
                     /locus_tag="POA77_001909"
     CDS             16154..17161
                     /locus_tag="POA77_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDI6365354.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17279..18523)
                     /gene="mtr"
                     /locus_tag="POA77_001910"
     CDS             complement(17279..18523)
                     /gene="mtr"
                     /locus_tag="POA77_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDI6365355.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18677..20566)
                     /gene="deaD"
                     /locus_tag="POA77_001911"
     CDS             complement(18677..20566)
                     /gene="deaD"
                     /locus_tag="POA77_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDI6365356.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20559..20639)
                     /gene="yrbN"
                     /locus_tag="POA77_001912"
     CDS             complement(20559..20639)
                     /gene="yrbN"
                     /locus_tag="POA77_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDI6365357.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20746..21630)
                     /gene="nlpI"
                     /locus_tag="POA77_001913"
     CDS             complement(20746..21630)
                     /gene="nlpI"
                     /locus_tag="POA77_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDI6365358.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21739..23874)
                     /gene="pnp"
                     /locus_tag="POA77_001914"
     CDS             complement(21739..23874)
                     /gene="pnp"
                     /locus_tag="POA77_001914"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI6365359.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24121..24390)
                     /gene="rpsO"
                     /locus_tag="POA77_001915"
     CDS             complement(24121..24390)
                     /gene="rpsO"
                     /locus_tag="POA77_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI6365360.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24539..25483)
                     /gene="truB"
                     /locus_tag="POA77_001916"
     CDS             complement(24539..25483)
                     /gene="truB"
                     /locus_tag="POA77_001916"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6365361.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25483..25884)
                     /gene="rbfA"
                     /locus_tag="POA77_001917"
     CDS             complement(25483..25884)
                     /gene="rbfA"
                     /locus_tag="POA77_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI6365362.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26048..28720)
                     /gene="infB"
                     /locus_tag="POA77_001918"
     CDS             complement(26048..28720)
                     /gene="infB"
                     /locus_tag="POA77_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI6365363.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28745..30232)
                     /gene="nusA"
                     /locus_tag="POA77_001919"
     CDS             complement(28745..30232)
                     /gene="nusA"
                     /locus_tag="POA77_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI6365364.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30260..30712)
                     /gene="rimP"
                     /locus_tag="POA77_001920"
     CDS             complement(30260..30712)
                     /gene="rimP"
                     /locus_tag="POA77_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI6365365.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30919..30995)
                     /locus_tag="POA77_001921"
     tRNA            complement(30919..30995)
                     /locus_tag="POA77_001921"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30959..30961),aa:Met,seq:cat)
     gene            31343..32686
                     /gene="argG"
                     /locus_tag="POA77_001922"
     CDS             31343..32686
                     /gene="argG"
                     /locus_tag="POA77_001922"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI6365366.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
BASE COUNT         8006 a         8844 c         8148 g         7746 t
ORIGIN      
        1 ttataaatga ataaagttac aaaaacagct attgtgggct tacttgcgct tttcgcaggt
       61 aatgccgctg caaccgatgg tgaaatagtt tttgatggcg agattttaaa atctgcttgt
      121 gaaatcaatg actctgataa gaaaattgaa gttgctcttg gtcactataa tgccgaacag
      181 tttcgttctg ttggcgatcg tagcccaaaa attccattca ctattccttt agttaactgc
      241 ccagtgactg ggtgggagca cgataacggc aacgttgaag cgtctttccg tttatggctg
      301 gaaacgcggg ataatggcac cgtccctaac ttccctaatc tggcaaaagt gggctctttt
      361 gctggcacag cggcaacagg tgtgggtatc cgtattgacg atgcggaaag cggaaacctt
      421 atgccactga atgctatggg gaatgataac acggtttatc agatcccagc ggactccaat
      481 ggtattgtca atgttgacct catcgcttac tacgtatcaa ccgttgaagc atcagaaatc
      541 accccagggg aagcagacgc tgtcgttaac gtaacgctgg attaccgtta atacgttacg
      601 gcgttatctg acctttcaga taacgccctt ttccttcctc tttctcgttg tatcaggttg
      661 aaaaataact atgtcaaaac gaacattcgc ggtgataata accttgttgt gtagcttctg
      721 tatcggccag gcgcttgcag ggggtatcgt tttacagcga acgcgagtga tctatgatgc
      781 cagccgcaaa gaggctgcgt tacctgtcgc aaacaaaggc gcagaaacgc cttatttact
      841 gcaatcatgg gtagataata tagatggtac aagccgtgcc ccatttatta taaccccacc
      901 gctatttcgt cttgaagctg gcgatgactc atcactgcga attattaaaa cagctgataa
      961 cctgcctgaa aataaagagt cgctgttcta cattaatgtt cgtgccattc cagcaaagaa
     1021 aaaatcagat gatgttaatg ctaacgagtt gacgctggta tttaaaacac ggatcaaaat
     1081 gttttatcgc cccgcacacc tgaagggacg ggtaaacgat gcgtggacat cactggaatt
     1141 taaacgtagt gaccattcac tcaatatata taacccaact gaatattacg tcgtatttgc
     1201 cggactggca gtcgataaaa ccgatctcac aagcaaaatt gaatatatcg cgcccggaga
     1261 acataaacag ttaccacttc ctgcatctgg cggaaagaac gtgaaatggg ctgcaatcaa
     1321 tgattatggc ggcagttccg ggacagaaac tcgtccactg caataacaaa tataaaaaac
     1381 acaggtcatc agggaatgcc acaacgacac caccagggac ataaacgcac accgaaacag
     1441 ttggcgctca tcatcaaacg ctgtttgccg atggtgctca ctggcagcgg catgctttgc
     1501 actaccgcta acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt
     1561 ggtatgcaaa caactgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact
     1621 tatcaggttg atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc
     1681 gccaatgcag agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt
     1741 attaaggtgg atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg
     1801 cttgaacaaa tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat
     1861 ttgagcattc cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt
     1921 tgggacgatg gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt
     1981 taccgccagg gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt
     2041 ggcccctggc gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg
     2101 aacactatca gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg
     2161 ggagaaagcg ccaccagcgg cagtattttt tccagctaca cctttactgg cgtgcaactc
     2221 gcttccgacg ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtacgcggt
     2281 atcgcaaaca gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc
     2341 aacgtgccag cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat
     2401 ttagaagtca cgattgaaga aagtgacggt acgcaacgtc gctttatcca gccttattct
     2461 tcattaccca tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat
     2521 cgcgctgatg caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat
     2581 ggtttgaata atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg
     2641 ctggggatcg gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac
     2701 agagctgaca cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtacgcag
     2761 tacatcaaag atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc
     2821 aacgatggct attttagttt tgatgaagcc aatacccgta attgggacta taacagtcgc
     2881 caaaaaagtg aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat
     2941 gcctccggtt cgcagcaaga ctattggggc aataacgata aaaacaggaa tatctcagtt
     3001 ggggtttccg gtcagcaatg gggaattggt tacagcctga attatcaata cagtcgctac
     3061 acggatcaaa ataatgaccg cgcactctct ttgaatctca gtattccgtt agaacgctgg
     3121 ttaccgcgta gccgggtttc ctatcagatg accagccaga aagatcgccc aacccaacat
     3181 gaaatgcgtc ttgatggctc actgctggat gatggtcgcc tgagctatag cctggaacaa
     3241 agtctggatg acgataacaa ccataacagt agcctgaacg ccagttaccg ttcaccttat
     3301 ggcaccttca gtgccggata cagctacggt aatgacagca gccaatacaa ttacggcgtt
     3361 accggcggcg tggttatcca tcctcatggc gtgacgctct cgcaatatct gggcaacgct
     3421 tttgcgctta tcgatgctaa tggggcttct ggcgtgagga tacaaaacta tccggggatt
     3481 gctactgatc cctttggcta tgcagtggtt ccttatctca cgacttacca ggaaaaccgt
     3541 ctctcggtag atactacgca gctgcccgat aacgtcgatc ttgagcaaac aacacagttt
     3601 gtggtgccca acagaggtgc aatggtagcg gcgcgtttca acgccaatat cggttatcgc
     3661 gtacttgtta cagtcagcga tcgcaacggt aaaccgttgc cctttggcgc tcttgccagc
     3721 aacgatgaga cggggcaaca aagtatcgtc gatgagggcg gcatactata tctctctggg
     3781 atatcgagta aatcacaaag ctggactgta cgctggggaa atcaggcaga tcaacaatgt
     3841 cagtttgctt ttagtacacc ggattcagaa ccaacaacct ctgtattaca aggcacggcg
     3901 cagtgccatt aaggataaaa aaatgaaaag agcgcctctt ataacaggac ttttgttgat
     3961 atccacatcc tgcgcttatg cctcctcagg agggtgtgga gctgacagca ctagcggtgc
     4021 gacaaattac agcagtgtgg ttgatgatgt tacggtgaac cagacagata acgtgacagg
     4081 acgggagttt acctctgcca cgctaagtag cactaactgg caatacgcct gtacctgctc
     4141 tgcgggtaag gcagttaaac ttgtctatat ggtcagccct gtacttacca ccactggaca
     4201 tcaagcagga tattacaaac tcaatgatag tctggatatt aaaaccacat tacagacgaa
     4261 tgacattcca ggactcgtga ctgaccagac cgtttctgtt aacacccgat tcacacagat
     4321 aaaaagcaac accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc
     4381 tcgttatgga cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc
     4441 atttctcggc tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcatgggt
     4501 cgatggaatg ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca
     4561 gggaaatctc accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa
     4621 ttttggattc atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac
     4681 tccagtagac tttaatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt
     4741 gcaaatgcgc atcgacggta caactggggt agtagaccag tacaacctgg tcgccagacg
     4801 aagaagttca gacaatgcgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt
     4861 tgcaaatatt ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccat
     4921 caacatgcgc gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca
     4981 gggcaccgcc accattaccg tcatcgtgcg gtaaaagaag acgttgcgtt ttgattaacg
     5041 caacgcttcg gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct
     5101 tcacgccgtg aatttctgcc gccagcgccg ccgctttttt caacggcagt tctgcctgta
     5161 gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt
     5221 cgataatcag caccatttcg cctttgcgac ggttttcatc ttcttttacc cacgccagca
     5281 gctcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa
     5341 ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt
     5401 gggtggattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac
     5461 ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac
     5521 ccgcggcgct taacgcagtg atagcagcac acggcccggg tagcggcacc acgcaaatcc
     5581 ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg
     5641 catcggaaac cagcgcaatg ttctgccctt cttgcagctt cgccagcagc gtttcggctt
     5701 tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca
     5761 gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct
     5821 ctaacgcacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct
     5881 ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt
     5941 aatattgagc attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc
     6001 tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg
     6061 tggcacccat actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc
     6121 tgccttttat cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt
     6181 actcgccatt cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa
     6241 ccaactaccg caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga
     6301 gattaaactg gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc
     6361 tgccgattta gaacaaaacc agcaagcccg ttactggcag gcaaaaatcg atgccagcca
     6421 ggggcgtcct tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc
     6481 gaaagaaaag cagcagaata ttgacgccac ctggcaggcg ctctcctcca tgactcagga
     6541 acaggcgaat acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct
     6601 gcagcgcgtc tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga
     6661 ctggcaaaaa cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa
     6721 cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cactgaatgg
     6781 ccaggcagcg gtatttggtc gcactattca gcaaggcttt gaagcggcga aaaatatcgg
     6841 cactcagcca gtggcagctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc
     6901 tcagccgcaa accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac
     6961 cggtgaacag cctgcagccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc
     7021 ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc
     7081 acttagccag atcttaagcc aggttcagca ggatggcgcg agtattgtgg tcggtccgtt
     7141 gctgaaaaat aacgttgaag agttgctgaa gagcaacact ccgctgaacg tactggcact
     7201 gaaccagccg gagaatatcg aaaatcgcgt caatatttgt tacttcgcgc tttcaccgga
     7261 agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt
     7321 gctgatccca cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca
     7381 gaaactaggc ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc
     7441 gggtgttaac ggcggttctg gtattgcttt aacgggtagc ccgattactc ccagagcgac
     7501 aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca
     7561 gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat
     7621 cgcttttatc aaaccgatga tcgccatgcg taacggtagc cagagcggtg caacgctgta
     7681 cgccagctcc cgcagtgcgc aagggaccgc tggcccggat ttccgactgg agatggaagg
     7741 cttgcagtac agcgaaatcc cgatgctggc aggcggtaat ctaccgttaa tgcagcaggc
     7801 actcagcgcg gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc
     7861 ctggtcgctg gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg
     7921 taataccgga agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct
     7981 acagtaccaa caaggtcagg tagtccccgc cagttaacca ccaaacagac cggcgatgcg
     8041 tgggaagtac aagcgcgtcg ctggctggaa ggcaaaggac tgcgatttgt cgccgctaac
     8101 gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt
     8161 gtcgaggtac gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc
     8221 agcaaacaac acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt
     8281 tttgatactg tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg
     8341 attaaggatg cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag
     8401 aattaaagct tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc
     8461 ggatgccatc tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat
     8521 cctctgttgt ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat
     8581 caaccgtttc gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt
     8641 tgtcttaacg gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg
     8701 ggcgctgggt catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga
     8761 tattgttaaa gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg
     8821 atatgacggc ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc
     8881 ttcgcatcgt agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga
     8941 tctgatcgat aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt
     9001 atcgccaatc gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt
     9061 agtgggtacc gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca
     9121 ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat
     9181 taagaaagaa acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca
     9241 gtcaccaaac gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc
     9301 caacgaagtg tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa
     9361 cgatacgtgg atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc
     9421 gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga
     9481 acgtgaagcg aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac
     9541 cacagcgttt acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc
     9601 gtcttatcag gcatacaacg atgcaaaccg tagatcggat aaggcgttta cgccgcatcc
     9661 gacatgtttt ccctcaaatc agaacaacag cgccagcccg ccgacaatca caccagacac
     9721 tgccaccatc gcccctgtca gccataaggc tttcgccggg aacgctttgc gcagcataat
     9781 cagtgacggc aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc
     9841 catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa
     9901 caagcatcct gccaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc
     9961 ggcatggggg aataaccaga cgcgagcggc acccaacacc agtactgcaa ggatgtaaac
    10021 cgggatcgta ctccagaaaa gcgtccatag cgccctgccc cagcggctaa aaaaaccgcc
    10081 ctgtgcttcc ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca
    10141 tttttgcacc agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc
    10201 cgcaaaaccc cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg
    10261 attgcccatc cagaatgcca gcgcaccgcc catcgacacc tgttggcgac gcattcccgc
    10321 cgcgaccgga gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag
    10381 cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag
    10441 cacctgaatc aacgaaccga ggatcacccc cagcactgcc gctttccata ccgcgaggaa
    10501 atagatcatc gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat
    10561 agatttaccg atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca
    10621 tttcacatac cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg
    10681 aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt
    10741 atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc
    10801 tcttttgcgt aattctgaag agcttaaaat cgtcacacct tcatgcccgg gccgcatcgc
    10861 ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggtaa
    10921 taaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag
    10981 taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcgatta atgcttcttc
    11041 catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact
    11101 gaccaccagc atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag
    11161 cgtgtaatcg gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag
    11221 acaacaaaac acaatgtcga tgggatcggt gacctgcgcc agcgcgtcag tcagttgcgg
    11281 atcatgggga ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat
    11341 ggcgttaact ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt
    11401 tgcgcctgta atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc
    11461 tccacggctc atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgggaaa
    11521 ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat
    11581 cactgatgaa tttgaagatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg
    11641 acacgaagtg attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga
    11701 agccagcgtg accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct
    11761 gctgctaccg ggcggccatt caccggatta tctgcgtggg gacaaccgtt ttgtcacctt
    11821 tacccgtgat tttgtgaata gtggcaaacc ggtgtttgcc atctgtcacg gcccgcagtt
    11881 gctgatcagc gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat
    11941 tgatgttaaa aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca
    12001 gctggttacc agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct
    12061 gctcggtgcc tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg
    12121 tgaatttgat ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg
    12181 gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga
    12241 tctcaccttt aaactgcaca ccgcgaatta acgctaccgt tttcggctga ccgtttaccg
    12301 ttccggcaac tgccgcctgc ggcccgctca tctgcgcgtg gcgcgttttt tcttccgtca
    12361 aaatgtagaa ggcgactttc tgcgcatcaa aaagataaaa ggcattagcg caccagagtt
    12421 ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccagccaa cggctgatgg
    12481 cgatgagtgt ttccattgct gttctctttt atactgtggg cctgacctta acatgcgatg
    12541 cagacgatga caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc
    12601 gggatcacca cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa
    12661 gcactgcgcg ggaaaggaga actgacgctg gcgttttctg ctccagtggg cgatcgttcg
    12721 ctggcgttac gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg
    12781 gtagcggaag gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa
    12841 agcgattgaa atgctcgtga tactcaacca ggccggtaac gccattcagc gcgtcatccg
    12901 ctaaacgatg tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat
    12961 gcgccgccag ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg
    13021 cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc
    13081 cctgtccacg gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt
    13141 cgccctgcac atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg
    13201 tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt
    13261 ccgcatcact ttcgaatgag cgacgtagca gggcatcaat acccggcgca tcaatgggaa
    13321 tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt
    13381 cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat
    13441 ttgctccagt tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc
    13501 ttcaatcacc agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat
    13561 catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac
    13621 caccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat
    13681 acttaaccag cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg
    13741 ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg
    13801 tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag
    13861 ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt
    13921 ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa
    13981 atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt
    14041 gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc
    14101 gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac
    14161 caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag
    14221 ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga
    14281 cggttacgcc cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct
    14341 gatcctcgcc gacctcgcca tgctggaata cgccgccgag cgttatccgc atattgagcg
    14401 ccacgtatcg gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca
    14461 ttttgacgtt gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacaact
    14521 ggcacgggtc acacctgtac cactggaagt ctttgctttc ggcagcctgt gcattatgtc
    14581 ggaaggtcgt tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtaggcgc
    14641 gtgttctccg gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct
    14701 gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac gcaggttatc cgacgctatg
    14761 taaagggcgt tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct
    14821 caataccctg gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga
    14881 aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat
    14941 cgaccgttgt aaggccgatc cgcaaaactt cataccgcaa agcgcgtgga tggagacgct
    15001 cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg
    15061 agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa agagacgctg
    15121 gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg
    15181 gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc
    15241 gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc atcctctgaa
    15301 ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc
    15361 ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac
    15421 tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg
    15481 ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagctgggc
    15541 attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc
    15601 gcccgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc
    15661 attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc
    15721 aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct ggcatccatg
    15781 caggggctgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc
    15841 gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc aaacagcgac
    15901 tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt aaatatgctc
    15961 gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg
    16021 taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca
    16081 gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac
    16141 aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat
    16201 tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc
    16261 tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc
    16321 cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct
    16381 ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg
    16441 cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag
    16501 tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc
    16561 ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg
    16621 cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag
    16681 cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga
    16741 tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga
    16801 aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gatagcaacc gcgacgctga
    16861 gtttctgttt acctcaatgc agcaagcctt tgtgaagttg cgccgtggcg aaaccgggca
    16921 actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac agtatggtgt
    16981 gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc atggcttgca
    17041 gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga ttttcgacca
    17101 ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata
    17161 gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat
    17221 aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt
    17281 actgatacac cggcagtaaa ttaaagctcg ataaaatatg gaccagtgcg ttgccaacgc
    17341 caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat ttcgggctgc
    17401 caaagcgttt acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag
    17461 ctgctaaacc agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca
    17521 caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga
    17581 aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg
    17641 ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc
    17701 cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact
    17761 ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca
    17821 tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca
    17881 tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc aatgtcatca
    17941 gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc gcaggctgca
    18001 catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc cccagcacaa
    18061 tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg
    18121 caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat
    18181 gcagaattga accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa
    18241 tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa atctttggtg atagtgtcaa
    18301 aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca
    18361 tacagaacca ggtgaagatc agcgccgcca ttgaccagaa gaaccacgcc ccggacatga
    18421 ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc
    18481 caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct tctctccagt
    18541 gaaaaatagt gcgactgcgt tgttatgtat tgcactgtac cagtacacga gtacaaaaga
    18601 cagaaaaaaa gccccgatgg ttcaaatcgg ggctgtatat attattttac agactgtgtt
    18661 cgctgttcag cgatgattac gcatcgccac cgaaacgacg acgaccggta gagtcatcac
    18721 gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacgaccac
    18781 cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgctcgcca ccgaaaccac
    18841 gaccaccgcc acgacgctca ccgccagtat gcggctgcgc atcgcccagc aactgcatat
    18901 tcatcggctt gttcagaatg cgagtacgcg taaagtgttg cagtacttca cccggcatac
    18961 ctttcggcag ttcgatagtg gagtgagaag caaacagctt aatgttaccg atgtaacggc
    19021 tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca acaccatcat
    19081 cgcggcccac ttcaatgcgg taaagctgca tgtcaccaac atcacgacgt tcacgacgcg
    19141 gacgatcttc acggtcgcca cgtgggccac ggtcgttacg atcgcgcgga ccacggtcat
    19201 cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca atcagagtac
    19261 gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc tgctcttcac
    19321 cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt
    19381 ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg
    19441 cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca
    19501 gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg
    19561 tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca
    19621 ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca
    19681 gacgaccatc tttcaggcgt tccagtgtct gctcacgcag cgcctggttc atgtcaccgt
    19741 tcagcgcggc actgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcat
    19801 ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca
    19861 gtgcttcgtt tttacgcata ccccagacag tccagtagct ctggctgatg tcaggacggg
    19921 tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc
    19981 gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct
    20041 gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat
    20101 ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca
    20161 gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt
    20221 cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat
    20281 ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct
    20341 gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt
    20401 ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac
    20461 actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag
    20521 gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc
    20581 tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt
    20641 gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta
    20701 aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga
    20761 ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg
    20821 ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc
    20881 caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc
    20941 agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct
    21001 aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc
    21061 gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agtttctgct cggcgagata
    21121 aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc
    21181 ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta
    21241 gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt
    21301 gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat
    21361 tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa
    21421 cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag
    21481 gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc
    21541 gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc
    21601 aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga
    21661 acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc
    21721 ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc
    21781 aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac
    21841 ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac
    21901 gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac
    21961 aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat
    22021 ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc
    22081 tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag
    22141 ggcacggata acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt
    22201 gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg
    22261 ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat
    22321 gatctctttg gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg
    22381 ggaacctgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga
    22441 cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc
    22501 tttgattggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga
    22561 agaagaaccg ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc
    22621 catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg
    22681 acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg
    22741 gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc
    22801 ggtacccagc gttgcagtga ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc
    22861 gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg
    22921 accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc
    22981 gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc
    23041 gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg
    23101 gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt
    23161 taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca gttcattgat
    23221 gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca gcatctggtc
    23281 ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag taccggcaac
    23341 aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc
    23401 attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac cagacagaga
    23461 cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg ggttaacaga
    23521 aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat
    23581 cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc cttcacgacg
    23641 gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag
    23701 tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa caaataccgc
    23761 ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc
    23821 cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat
    23881 atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg
    23941 catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc
    24001 cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat
    24061 tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa
    24121 ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt
    24181 caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg
    24241 gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag
    24301 tgctacctga acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat
    24361 tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag
    24421 gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac
    24481 gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagat ggttaacatt
    24541 acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc
    24601 ccataccgat aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg
    24661 caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat
    24721 tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact
    24781 ccgcagccgg aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg
    24841 tcaccatccg ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg
    24901 cgccacaacc gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg
    24961 agcagtgaat ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa
    25021 cggtaatcgg gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt
    25081 ttttaccctg atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga
    25141 aagtatccag cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga
    25201 tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc
    25261 gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca
    25321 acatgccagt tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa
    25381 tccgtttcac tttttgcagc gcatcattgc tggacatacc ctgcgattta tccagcaaca
    25441 aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc
    25501 gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat
    25561 gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag
    25621 gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat
    25681 gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata
    25741 cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc
    25801 tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg
    25861 cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc
    25921 ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag
    25981 cgtcacatca ggcaatccat gccggatgcg gcataaacgc cttatcccgc atggaaccct
    26041 aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc
    26101 cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt
    26161 cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt
    26221 tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc
    26281 ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa
    26341 tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca
    26401 ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg
    26461 cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg
    26521 tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt
    26581 cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct
    26641 tcaggacgat gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga
    26701 gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg
    26761 caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta
    26821 cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac
    26881 ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc
    26941 ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga
    27001 ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa
    27061 cttccgcctg cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag
    27121 atacgtgtac gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga
    27181 gttcgttctt aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca
    27241 ccaccggtac ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc
    27301 cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca
    27361 ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt
    27421 caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg
    27481 ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca
    27541 caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt
    27601 ccagctcgtt ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg
    27661 cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt
    27721 tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt
    27781 cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcaggg
    27841 aagaaccttt acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat
    27901 cagcttttga ttcagcgtgt ttgttacctt tcttcggacg cgctgctttc gcgttacgac
    27961 cacggccacg gccgccttcg acttcacgat cgctttcgtc ttcggcctgg cgagcatgtt
    28021 gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt
    28081 tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt
    28141 tacgacgcgc ttcttcttca gctttacgct tgagctctgc agcttcctgc tcacggcggg
    28201 ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt
    28261 ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    28321 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    28381 cttccgcttc acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc
    28441 gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg
    28501 taccaggaat gttaagggtg ctgcgtgttt tacgctgcag cgtcaatttg tccgggcctg
    28561 aatttttctg attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt
    28621 cagcagactt ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct
    28681 gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag
    28741 tttattacgc ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg
    28801 ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat
    28861 cttccagcgt acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt
    28921 caaggttcag cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg
    28981 caatggtggc cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa
    29041 ggccttcgat ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga
    29101 agccttcttc taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg
    29161 tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca
    29221 cgttgagttc ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa
    29281 tcgcctgcgc caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca
    29341 ccacgataga agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat
    29401 catcccacag gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac
    29461 gcgcgccacg catacctaca caggcaccta ccggatcgat acgtttatcg ttggttttca
    29521 ccgcgatttt cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt
    29581 cgccgatttc tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag
    29641 tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc
    29701 ggtcgccagg gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag
    29761 cattgttgcc cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga
    29821 tgatttcacc ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca
    29881 ctttctgcac gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact
    29941 caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt
    30001 caagggtgat ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg
    30061 tgtcaaaatc accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt
    30121 ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta
    30181 gcgccttttc attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct
    30241 tttcacctca tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca
    30301 gcgcgaacac ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt
    30361 tgataacgcc ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct
    30421 cttctccgac aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg
    30481 aggagacttc caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta
    30541 cctggtggct cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga
    30601 tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg
    30661 cctcaactgg cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc
    30721 ccaagacata aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc
    30781 cgataaatcg gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt
    30841 tccagaagga ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa
    30901 aaggactcta agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat
    30961 gagcccgacg agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg
    31021 ttttgggtaa aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc
    31081 tgcaggcatt atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat
    31141 gaatatccag ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaagc
    31201 gcattatttt acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg
    31261 aaaactcatt tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt
    31321 taattaagca gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg
    31381 tattggtatc gctttttccg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca
    31441 aaagggagcg gttccttatg catatactgc aaacctgggc cagccagacg aagaggatta
    31501 tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg
    31561 ccgcaaacaa ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac
    31621 caccggcggc ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggtaccat
    31681 gctggttgct gcgatgaaag aagatggcgt gaatatctgg ggtgacggta gcacctataa
    31741 aggaaacgat atcgaacgtt tctatcgtta tggtctgctg accaatgctg aactgcagat
    31801 ttacaaaccg tggcttgata ctgactttat tgatgaactg ggcggccgtc atgagatgtc
    31861 tgaatttatg attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cctactccac
    31921 agactccaac atgcttggtg caacgcacga agcgaaggat ctggaatacc tcaactccag
    31981 cgtcaaaatc gtcaacccaa ttatgggcgt gaaattctgg gatgagagcg tgaagatccc
    32041 ggcagaagaa gtcacagtac gctttgagca aggtcatccg gtagcgctga acggtaaaac
    32101 ctttagcgac gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggcct
    32161 gggcatgagc gaccagattg aaaaccgtat catcgaagcg aaaagccgtg gtatttacga
    32221 ggctccgggg atggcactgc tacatatcgc ttatgaacgt ctgttgaccg gtattcacaa
    32281 cgaagacacc attgagcagt atcatgcaca tggtcgtcag ttgggccgtc tgctgtacca
    32341 ggggcgttgg tttgactccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc
    32401 cagccagatc actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct
    32461 gaataccgtc tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga
    32521 ctcggtgttc tcgccagatg atcgtattgg tcaattgacc atgcgtaatc tggatatcac
    32581 tgatacccga gagaaacttt ttggttatgc caaaactggc ctgctctcct cctctgccac
    32641 ttcaggcgtg ccgcaggtgg agaatctgga aaacaaagtt aagtaatatc atagctttcg
    32701 tgctaaaggc cgcaaatgcg gccttttttt acggctaaga gaaa
//
LOCUS       DAMZII010000041        32588 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZII010000041 DAMZII010000000
VERSION     DAMZII010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.041"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..615
                     /locus_tag="POA77_001923"
     CDS             <1..615
                     /locus_tag="POA77_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDI6365367.1"
                     /translation="LAIGAHPDDIELGCGASLARLAQKGIYIAAVVMTTGNSGTDGII
                     DRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISALEDIIKNQIPSDVEIMRVYT
                     MHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSFMPQVFESVKEEYFTVKLAA
                     LKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            625..1821
                     /locus_tag="POA77_001924"
     CDS             625..1821
                     /locus_tag="POA77_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6365368.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1799..2380
                     /locus_tag="POA77_001925"
     CDS             1799..2380
                     /locus_tag="POA77_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6365369.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2382..3155
                     /locus_tag="POA77_001926"
     CDS             2382..3155
                     /locus_tag="POA77_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDI6365370.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLYQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3341..3616
                     /gene="frmR"
                     /locus_tag="POA77_001927"
     CDS             3341..3616
                     /gene="frmR"
                     /locus_tag="POA77_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDI6365371.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3651..4760
                     /gene="frmA"
                     /locus_tag="POA77_001928"
     CDS             3651..4760
                     /gene="frmA"
                     /locus_tag="POA77_001928"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDI6365372.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4853..5686
                     /gene="fghA"
                     /locus_tag="POA77_001929"
     CDS             4853..5686
                     /gene="fghA"
                     /locus_tag="POA77_001929"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI6365373.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIIVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5811..6350)
                     /locus_tag="POA77_001930"
     CDS             complement(5811..6350)
                     /locus_tag="POA77_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDI6365374.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6452..7663)
                     /gene="mhpT"
                     /locus_tag="POA77_001931"
     CDS             complement(6452..7663)
                     /gene="mhpT"
                     /locus_tag="POA77_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDI6365375.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7839..8852)
                     /gene="dmpG"
                     /locus_tag="POA77_001932"
     CDS             complement(7839..8852)
                     /gene="dmpG"
                     /locus_tag="POA77_001932"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDI6365376.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8849..9799)
                     /gene="mhpF"
                     /locus_tag="POA77_001933"
     CDS             complement(8849..9799)
                     /gene="mhpF"
                     /locus_tag="POA77_001933"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDI6365377.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9796..10605)
                     /gene="mhpD"
                     /locus_tag="POA77_001934"
     CDS             complement(9796..10605)
                     /gene="mhpD"
                     /locus_tag="POA77_001934"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDI6365378.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10615..11481)
                     /gene="mhpC"
                     /locus_tag="POA77_001935"
     CDS             complement(10615..11481)
                     /gene="mhpC"
                     /locus_tag="POA77_001935"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDI6365379.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11499..12443)
                     /gene="mhpB"
                     /locus_tag="POA77_001936"
     CDS             complement(11499..12443)
                     /gene="mhpB"
                     /locus_tag="POA77_001936"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDI6365380.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12445..14109)
                     /locus_tag="POA77_001937"
     CDS             complement(12445..14109)
                     /locus_tag="POA77_001937"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDI6365381.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTVGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14186..15133
                     /gene="mhpR"
                     /locus_tag="POA77_001938"
     CDS             14186..15133
                     /gene="mhpR"
                     /locus_tag="POA77_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDI6365382.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQDRKELIEMLASRPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15210..16292
                     /gene="lacI"
                     /locus_tag="POA77_001939"
     CDS             15210..16292
                     /gene="lacI"
                     /locus_tag="POA77_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDI6365383.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16415..19489
                     /gene="lacZ"
                     /locus_tag="POA77_001940"
     CDS             16415..19489
                     /gene="lacZ"
                     /locus_tag="POA77_001940"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDI6365384.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19541..20794
                     /gene="lacY"
                     /locus_tag="POA77_001941"
     CDS             19541..20794
                     /gene="lacY"
                     /locus_tag="POA77_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDI6365385.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSIRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(20873..22054)
                     /gene="cynX"
                     /locus_tag="POA77_001942"
     CDS             complement(20873..22054)
                     /gene="cynX"
                     /locus_tag="POA77_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDI6365386.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQVVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSAQPTIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22060..22530)
                     /gene="cynS"
                     /locus_tag="POA77_001943"
     CDS             complement(22060..22530)
                     /gene="cynS"
                     /locus_tag="POA77_001943"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDI6365387.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22561..23220)
                     /gene="cynT"
                     /locus_tag="POA77_001944"
     CDS             complement(22561..23220)
                     /gene="cynT"
                     /locus_tag="POA77_001944"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDI6365388.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23330..24229
                     /gene="cynR"
                     /locus_tag="POA77_001945"
     CDS             23330..24229
                     /gene="cynR"
                     /locus_tag="POA77_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDI6365389.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24269..25552)
                     /gene="codA"
                     /locus_tag="POA77_001946"
     CDS             complement(24269..25552)
                     /gene="codA"
                     /locus_tag="POA77_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDI6365390.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINICFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25542..26801)
                     /gene="codB"
                     /locus_tag="POA77_001947"
     CDS             complement(25542..26801)
                     /gene="codB"
                     /locus_tag="POA77_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDI6365391.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27038..28924)
                     /gene="prpE"
                     /locus_tag="POA77_001948"
     CDS             complement(27038..28924)
                     /gene="prpE"
                     /locus_tag="POA77_001948"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDI6365392.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARCIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(28964..30415)
                     /gene="prpD"
                     /locus_tag="POA77_001949"
     CDS             complement(28964..30415)
                     /gene="prpD"
                     /locus_tag="POA77_001949"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDI6365393.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30449..31618)
                     /gene="prpC"
                     /locus_tag="POA77_001950"
     CDS             complement(30449..31618)
                     /gene="prpC"
                     /locus_tag="POA77_001950"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDI6365394.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(31663..32553)
                     /gene="prpB"
                     /locus_tag="POA77_001951"
     CDS             complement(31663..32553)
                     /gene="prpB"
                     /locus_tag="POA77_001951"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDI6365395.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
BASE COUNT         7559 a         8993 c         8573 g         7463 t
ORIGIN      
        1 cttgctattg gcgcacatcc tgacgatata gaattaggct gtggcgcatc gcttgctcgt
       61 cttgcgcaaa aaggaattta tatcgcagcc gtggtgatga ctaccggcaa ctctggcaca
      121 gatggaataa tcgatcgcca tgaagaatcg cgcaacgccc taaagatatt agggtgccac
      181 caaactattc atcttaattt tgctgacacc cgcgctcatt tacagctcaa tgatatgatt
      241 tccgccctgg aagacatcat taaaaatcaa attccttctg atgttgaaat catgcgggta
      301 tataccatgc atgatgccga ccgccatcag gatcatctcg ctgtttatca agcttcaatg
      361 gttgcctgcc gcactattcc acaaattctc ggctacgaaa ccccaagtac ctggctttca
      421 tttatgcctc aggtttttga atccgttaaa gaagaatatt tcacagtcaa acttgctgca
      481 ttaaaaaaac ataaaagcca ggaacgacgt gattatatgc gtcatgatcg cctgcgtgca
      541 gttgcacaat ttcgcgggca acaggtcaat agcgatctgg gtgaaggctt tgtaattcat
      601 aaaatgattc tttgagcaaa ttgcatgaaa acctggatat ttatctgtat gtccatagca
      661 atgttgctat ggtttttaag tacgctaaga cgtaaaccca gtcaaaagaa aggctgtatt
      721 gacgccatta tacctgcgta taacgaaggc ccgtgtctgg cgcagtcact ggataatcta
      781 ctgcgtaacc cttatttttg ccgggtaatt tgcgttaacg acggctccac ggacaatacc
      841 gaagcggtca tggcggaagt caaacgcaaa tggggcgacc gctttgttgc cgtcacgcaa
      901 aaaaataccg gtaaaggtgg tgcgctgatg aatggcctca attatgccac ctgcgaccag
      961 gtttttttaa gtgatgccga cacctatgtt ccgcccgatc aagacggaat gggctatatg
     1021 ctggcagaaa ttgagcgcgg tgctgatgcc gtaggcggca ttccctctac tgcgttgaaa
     1081 ggcgcggggc tgttaccgca catccgcgcg accgtaaagt tgccgatgat tgttatgaag
     1141 cgcacgctac agcagctcct gggcggcgca ccgtttatta tcagcggtgc ctgcgggatg
     1201 ttccgtactg atgtattgcg taagttcggt ttctcggatc gtactaaagt cgaagacctt
     1261 gatctcacct ggacattagt ggcaaacggc taccgtattc ggcaggcgaa tcgctgcatc
     1321 gtatacccac aggaatgcaa cagtccgcgt gaggagtggc gtcgctggcg gcgttggatt
     1381 gtgggatacg cggtctgtat gcgcctgcat aaaagacttt tatttagccg cttcggtatc
     1441 ttcagtatat ttcctatgct gttggttgtg atttatggcg ttgggattta tctcactacc
     1501 tggtttaatg aattcatcac caccgggccg catggtgtgg tgttggcaat gtttccgctt
     1561 atctggatcg gcgtagtttg tgttattggt gcttttagcg cctggtttca tcgttgctgg
     1621 ttgttggtgc ctttagcgcc gctttccgtt gtgtatgtat tattagctta tgccatctgg
     1681 attatttatg gacttattgc cttttttact ggacgcgaac ctcagcgcga caaacccacc
     1741 cgctattccg cactggtgga agcgtcaacc gcttattccc aaccttctgt cacaggaact
     1801 gaaaaactat ctgaagctta atgttaaaga gaaaaatatt cagattgcag accaggtaat
     1861 tattgatgaa actgcaggtg aagttgttat cggcgcgaat acccgtattt gtcatggtgc
     1921 cgttattcag ggtccggtag tgattggcgc aaactgcctg ataggtaatt atgcgtttat
     1981 tcgtcctggc acaataatca gcaatggtgt aaaaattggt tttgccaccg aaattaaaaa
     2041 tgcggttatt gaagcggaag caacgattgg tccgcaatgt tttattgccg actcggtagt
     2101 tgcaaaccag gcatatttgg gcgcacaagt acgtaccagt aatcatcgtc tggatgaaca
     2161 gcctgtgtct gttcgaactc cagagggaat tatcgctacc ggatgcgata aattaggttg
     2221 ttatatcggg cagcgttcac gccttggcgt acaagttata attttgcctg ggcgaattat
     2281 ttccccgaac acgcaacttg gcccgcgcgt gattgtagaa cgtaatttac ctgccggaac
     2341 ttattcactc cgacaagaac ttatccgtac aggagattaa aatgataaaa cggacgttat
     2401 tagcggcggc catttttagc gcattgcccg cttatgccgg gttaacttcc attaccgcgg
     2461 gctacgattt taccgattat tctggcgatc atggcaaccg taatttagcg tatgctgaac
     2521 tggtggcgaa agttgaaaac gcaacgctgc tttttaatct ttaccagggg cgtcgtgatt
     2581 atgaaactga aaatttcaat gccactcgcg gtcagggtgc ggtctggtat aaatggaata
     2641 actggctgac aacccgaacg ggtattgcct ttgcggataa tacgccggtc tttgcccgcc
     2701 aggattttcg tcaggatatt aacctggccc tgttgccaaa aacgcttttc acgaccggtt
     2761 atcgctacac taaatattac gatgatgtcg aagtcgatgc ctggcaaggc ggcgtatcac
     2821 tctatactgg cccggtgatc accagctacc gctataccca ttatgactcc agcgatgcag
     2881 gtggtagtta tagcaatatg atttccgtgc gtctgaatga cccgcgcggc actggttata
     2941 cgcaactatg gctaagccgc ggaacaggcg cttacaccta tgactggacg ccagaaacac
     3001 gctacggcag catgaagagc gtcagtttgc aacgtattca accgctgact gagcaactta
     3061 atctcgggct gacggcaggt aaagtgtggt acgacacccc aaccgatgat tataacggtc
     3121 tgcaacttgc agcccatctg acctggaaat tctgattcct tctgccgccc gctatccggg
     3181 gcggccttcc ctgccgatta gcccccccct ttcctctttg ttttccgacc acattcaccg
     3241 gataaatttt attctccagt gttatatact ataggggggt atgcattgac atatagcata
     3301 cccccctata gtatattgcg tgcagatgat gaggtgcgaa atgcccagta ctccggaaga
     3361 gaagaaaaag gtccttactc gagttcgtcg tattcgaggg cagattgatg ctctggaacg
     3421 gtcgctggag ggtgatgccg aatgccgtgc catactccaa cagatcgctg ccgttcgggg
     3481 cgcggctaac gggctgatgg cagaagtgct tgaaagccat atccgggaaa cgtttgaccg
     3541 aaatgactgc tacagccgcg aagtcagcca atccgttgac gacactattg aactggttcg
     3601 agcctatctt aaatagctga atctattacc atattgagga agagcgagag atgaaatcac
     3661 gtgctgccgt tgcatttgct cccggtaaac cgctggaaat cgttgaaatt gacgttgcac
     3721 caccgaaaaa aggtgaagtg ctgattaaag tcacccatac cggcgtttgc cataccgacg
     3781 catttaccct ctccggggat gacccggaag gtgtattccc ggtggttctc ggtcacgaag
     3841 gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg
     3901 tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta
     3961 acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt gatgccagac ggcaccaccc
     4021 gtttttctta caacgggcag ccgctttatc actacatggg gtgctctaca ttcagtgaat
     4081 acaccgtggt cgcggaagtg tctctggcca aaattaatcc agaagcaaac catgaacacg
     4141 tctgtctgct gggctgtggc gtgaccaccg gtattggcgc ggtgcacaac acagctaaag
     4201 tccagccagg tgattctgtt gccgtgtttg gtcttggcgc gattggtctg gcagtggttc
     4261 agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat cgataccaac ccgaagaaat
     4321 tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa cccgaatgac tacgacaaac
     4381 cgattaaaga tgtcctgctg gatatcaaca aatggggtat cgaccatacc tttgaatgca
     4441 tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt
     4501 cggtgatcat cggggtggca ggttctggtc aggaaatctc cacccgtcca ttccagttgg
     4561 tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac
     4621 cgggtatggt tgaagatgcg atgaaaggtg atatcgatct ggaaccgttt gtcacgcata
     4681 ccatgagcct tgatgaaatt aatgacgcct tcgacctgat gcatgaaggc aaatccattc
     4741 gaaccgtaat tcgttactga tttcccgcag gtataccccg tccacttcag acggggttct
     4801 taatactctc cccgggcagc cgtccggggg attaaccgtg agataatgac tgatggaact
     4861 cattgaaaaa catgccagct ttggcggctg gcaaaatgtg tatcggcatt attcccaatc
     4921 attgaaatgt gaaatgaatg tcggcgtcta tctcccgcca aaagccgcaa gtgaaaaatt
     4981 accggtgctg tactggcttt cgggcctgac ctgcaatgag cagaatttca ttactaaatc
     5041 ggggatgcag cgttacgcgg ctgagcacaa cattattatt gttgcgccgg acaccagtcc
     5101 gcgaggcagt catgtcgcag atgccgaccg ttacgatctc gggcaaggtg ccgggtttta
     5161 cctgaacgcg acgcaagcgc cgtggaatga acattacaaa atgtatgact atatccgcaa
     5221 cgagctgccg gatttagtga tgcatcattt tccggcaacg gccaaaaagt ctatctctgg
     5281 tcattctatg ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag atgaatatgt
     5341 cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc
     5401 ctttgccgca tatcttggtg aaaataaaga tgcctggttg aattacgatc cggtgagtct
     5461 tatttcacaa ggtcaacgcg ttgcggaaat catggttgat caggggttga gtgatgattt
     5521 ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa
     5581 gacgttaatc cgttatcacg agggttacga ccacagctat tattttgtct ccagttttat
     5641 tggcgagcat attgcctacc acgccaataa actgaatatg cgttgataat agtgcacgac
     5701 tgccggatgc ggcgtgaacg ccttatccgc ctacatttcg cccgcaggcc gtaggcatga
     5761 taagacgcgc atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt ttaccacatc
     5821 aaatcatcag gcactttgaa gtcggcatat gggtcgtctt catcctgctc ttcggcgctg
     5881 agcgcgctgt gcaacactat actgctggca tcacgctgag ctattttatc ggcaacgctg
     5941 gcgggaataa tggcgtattc accttcgcta ttgttatcga ccaacaagcg agcaatcgcc
     6001 agacgaccat taatcaactg cgcctgtgtg agcttatcga caaaaatctt cttaatcaga
     6061 ttgccgtcag tgaagttaaa accaatatcg ccattggcaa tggtgattcg gttcatttca
     6121 atgagctgct tcacctgagc tttatattct ttcgccaacg cagcttgttt ttgttgctcg
     6181 ctaagctgtt tatcacgctc aagctgtgcc tttttatttt cttctaccgc cgcccgagct
     6241 tcacgcgcct gaacgcgcga ttttttcgcc gttctctcca ccttcgccgc ttttttgctg
     6301 gtgactaatc ctgctttgag caactgctct tgtaaggtaa gttttgccat ctttgtttcc
     6361 aaacccgctg aataattttt agcgagtata cctgtaaacg ccgtgggtgt attgggggca
     6421 actccctttc gcccctgctt aataaggcgc gtcaggcatc ggcacacggc tgcattcttg
     6481 atctccgact catcaaaata aacaccgcca acccagcaac aagaataccc ggtgcagaag
     6541 cggccattac gccgaccgtg ccagtgccta atgccagcat tttcccggcc agtaacggac
     6601 cgctcatagc ccccagacgc cctaccgcca cggctgttcc cacacctgtt gcgcggatct
     6661 gcgaactgta aaacaacggt gccagggcat acaaaacgct ttgcccacct gtcgcaaaca
     6721 accccgcgac aaatcccgcc agcaacatac cgttaaatga cgacaccgtt ccaagtgcca
     6781 gcagcgaagc taacatgccg ctataaatca gtagcgacat ggtcactgga cgcagcttat
     6841 ccatcaatgc gcccaacatt aacgtcccgc ttgccgcccc catttgcagg gcaaacatca
     6901 cccctgccgc ctgcgatggc tggaatcctt gctccaccaa aagtagcggt agccagttga
     6961 tcaacatata aaccaccagc agagtgaaga aataacacaa ccacagcagc agcgtcgcgg
     7021 ttgccgtttc tggcgcaaat aaggcacgca gtggtggcgc agcctgtttt tcgccagcga
     7081 aaaccgctga ctccggcagc cagcgcatta atagcggcac cagaatcaac ggcaccacac
     7141 cacctaccca aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg
     7201 ccagcgccgc gccaatggga acaccgcaat acatcaggct cactgccgtc ccacgaaaac
     7261 gtggacccgc ggcttcagac gtcagggcga taagattcgg caacgccgcc cccagcccga
     7321 caccggtcat cagccgcgca aagaccagtg aggggaaatc ccaggcaatc gccgttgcca
     7381 gtgagaacaa accaaacagc gcaactgagc caatcaaaat acgcttacga ccataacggt
     7441 ctgccagcat cccgccaacc aacgcgccgg gtagcaaacc gagtattccg gcgctaaata
     7501 tccagcccat ttgcatttta tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc
     7561 cagccgcctg aagatccagc ccttccatca gagcgaccaa aaaacaaagc ccgatggtca
     7621 gcatcaggcg ggatgaagat gatgaagggg tacgagtcga cataagatga acctcattgc
     7681 gagtgcaggt gaatcgcgac tgatacattg ccagccgcgt aatcagaact atttaatgac
     7741 tgcctacggg cgaaatgtag gccggataag gcgttcacgc cgcatccggc tgtcgtgctg
     7801 ggatgggcat acaaaatgca cgttatcggc agtcatcatt atttgttgtt gcgcagatcc
     7861 agcgccacgt cgacgatcat atcctcctgg ccgccaacca tccggcgttt gcccagctca
     7921 acgagaatat ccaccgcact taagccataa cgcgccgccg ccgtttcaca gtgacgcagg
     7981 aagctcgagt aaacaccagc gtatcccagc gccagcgttt cgcgatcgac tcgtaccggt
     8041 cgatcctgca acggacgcac caggtcgtcg gcggcatcca ttaacgcata gagatcggtc
     8101 ccatgctgcc agcccagttt atccgcggcg gcaataaaca cttccagcgg tgcgttacct
     8161 gcgcccgcgc ccattcccgc gaggctggcg tcgattcggt cgcagccctc ttccaccgcc
     8221 gcgatagagt tcgccacgcc aagactcagg ttatggtgag cgtgcatgcc agtttgcgtt
     8281 tctggtttca gctctgcttt cagggcgcgg aaacggtcac ggatatcgct catgttcatc
     8341 gcaccgccag aatccaccac ataaatacag gtcgcaccgt agccttccat cagctttgcc
     8401 tgcttggcga gattctccgg cgtggtcata tggctcatca tcagaaaacc aacggtgtcc
     8461 attccgagct cgcgggcata ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc
     8521 gttgccacac gaaccacccg cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg
     8581 ccgattcctg gcagcaacaa cgtcgcgatt ttggcgtgct tcaccacatc cgccgctgct
     8641 tcaatccatt caaggtcgct atgtgcgccg aaaccatagt taaagctgga accttgcaaa
     8701 ccgtcgccgt gggccacttc aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc
     8761 tggcgaacgt tttccagcga atactgatga cgaatggcgt gcataccgtc acgcaatgtg
     8821 acgtccgaga tataaagttt tttaccgttc atgccgcttc tcctgccttg cgcgccagtg
     8881 actgggccat tttttccgct gtcgccagcg cactggaagt cataatatcg aggttgcccg
     8941 cataggcagg cagataatgc gctgcgcctt cgacttccag ccagaccgct gttttcagcc
     9001 cggagaactg acccacgccc ggcaaattaa ccggtttatc ctgcgggata acttcaaact
     9061 gcacgcgctg tttcaggcga tatcccggta cgtaagcctg caccgcctca gccatttcat
     9121 tgattgaggc ttcgatatca tcttgtgaag cttcgtcgct caatacatac accgtgtcac
     9181 gcatcatcaa cggtggctct gctgggttaa gcacaataat cgccttccct tttgccgcgc
     9241 cgcccaccac ttcaatggct cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg
     9301 tgccaggtcc ggcagattta ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg
     9361 ccacgcgtga aactgccgcc accattggaa tggtggcctg gccgccgcag gtgaccatgt
     9421 tgacgttcag ttgatcgacg ttcgcctcga ggttaaccac cggcacgcag taagggccga
     9481 tggcagcagg cgtcaggtca attaagcgaa tatccggttt cgcttcgcgt aaagcggcat
     9541 cgtttttcac atgagcaccg gcgctggtcg catcaaatac aatgtcgata tcagcaaatt
     9601 caggcatgtt catcagcccg atcacccctt catgggtagt ggcgacgccc atacgtctgg
     9661 cacgcgccag accgtcggac tgaggatcaa tgccgaccat caccgccatc tccagatgct
     9721 gaccgtgacg caaaatttta atcatcagat cggtgccaat gttgccagaa ccgataatgg
     9781 cgactttacg cttactcatg acagacttcc ttttggggct gcgcttgaaa atgtcgcagc
     9841 aactgaacct atgccttcaa tatgggcttc aaaacgatcg cccgcattca ctgccaccat
     9901 cggacctaat gccccggtaa gaatgatatc tccggcgcgc agcggttcac ccaggctggc
     9961 cattttgcgt gccagccaga cggcggcatt aagcggatgt cccaggcatt cgctgccgcg
    10021 cccgctggaa acctcttcgt tattacgcgt catcttcatg gcgcagtttt tcaggtctaa
    10081 ccccgccgga cgttgcgccg gaccgccgat gacatacacc ccgcaggaag cgttatctgc
    10141 cacggtatcg acaaactgaa tcgaccagtc gcgaatgcgg ctacccacca cctccagcgc
    10201 cggaagtacc cattcaatgg cgttatacaa ttcgtcgaag gtgatatcgg ttgcgggcaa
    10261 atcgcggttc aacaccagtg cgatctccgc ttcaatgcgg ggttgcagaa cacgggaaaa
    10321 aggaatgatt tcgttatcgc cataacacat gtcggcaaat aatgtcccga aatccggttg
    10381 atcaacgccc agttgttgct gcactttcgg atgcgtcagg cccactttac gccctaccac
    10441 gcgacgcccc tgcgcaacgt catattgcac atttatgtgc tgaatggcgt aggccgcttc
    10501 agcgttatcg ataccaatca gatcgcgcag cggcgcaatc gcttcgccct gctctgcggc
    10561 gcggcgtaaa tccgccgcca gttgctcaag agtatgcttc gtcatgacca ttccttaagg
    10621 gcgtgcgagg aaattcagca ccaattgatt gaaagcgtcg gcatgttccc actgcgccca
    10681 gtgaccacag tcgcggaaga tatgcagttc agaaccggca atgccggaca gcagacgcag
    10741 acccgcatcc atcggcacaa agcggtcgtt gcgcccccac acaatcaggg tttgcgcttt
    10801 gatttccgcc agacgtgggc caaaatccgg gaactgtttc ggattagctt ccaggctctt
    10861 aacgaagttt tccaggtgat cgcggcgcga cagcatatta ttcaggcgcg cttcaaacag
    10921 ggcgtcggtc aaatcgctgg tatcgaaaac gaagatatcc atcatcagct tcaggttttc
    10981 gatagtcggc tgacgataaa gctgattcag tcgcttaata ccttcggttg gcatcggcgt
    11041 aaacaaactc atgccgcccg taccgccgcc catcagcacc agtttgccga cgcgctccgg
    11101 ccagttaagg gtgaacgcca cagaactatg gccgcccatc gagttgccca gcaggtggat
    11161 tttggcgata tccagttgat ccaccacgct tttcaggatt cgtgcattaa gatccgatcg
    11221 tgaaccacta ttaacgatcg aatcgctttt gccccaaccc ggacaatcca gcaggatcac
    11281 ccgatagccc gcctctacca gcggatcgat attgcggctg aagttcgccc agccagtagc
    11341 acccgggccg gaaccatgca gcaggacaac ggtttcgtcg ccttgtccgc agtcattaaa
    11401 atggatgcgc agcgttttac ccgcttcttc tacattcaga aaacggctgg tggcggcttc
    11461 ggtttgtggc tgataactca tcatcttctc ctgcatattc agttctctgt tctggcgctt
    11521 aacgagccaa atccggcaat ccactccggg attgggcggt aataacgccc ttcgctacgc
    11581 cagttgccaa acgcagaaat agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc
    11641 gactttccgg caatagcgga aagctcttcg ttactgacgg catccagttc ctgtatgcgt
    11701 ccctgctcca gcaaagtcat gaactggtta tcccaaatcg ggttgagcgg atgcagagtt
    11761 ctctgatcct caacaaactt ctcagcggcg ctaatcaccc gttgctgacg caattcgcgc
    11821 tcactggcgg gtaaatcttt cccgctcccc aacagacggt cgcgcatatg ggcatcggct
    11881 ttcgccagtt cgggcaccgg cggctgatgg gaaagcccac cggaacccag gaacagcacg
    11941 cgtttattga gagtgctggt gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg
    12001 aaaccgggca gcggcgtggc gacaccgttg atgaacacag gcagaactgg caccttatcc
    12061 agcccaccga gcaggaactc cagcggctgg gcgaacccgt ggtccacctg catacagtaa
    12121 gaaacggcaa gatcgatccc gctcttcatg acagcatgcg cacaggcctc cgccagctcc
    12181 acaggcacgg gcagctctcc tgccgcactg ccgaaatcac caattgccgt cgctccaacg
    12241 cctaaacaga acggtggcat cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac
    12301 agcaccacca gttcagggga gaaagccgca atacgctcgc gggcgctggc aatcacgcca
    12361 ttgacctcat cgagtacctc ttgcgccggg tcgacatacc ccaccaacgg cgagtgggaa
    12421 agacagtgaa gataagcgtg catatcaggc taccttttcg acagaaacgt cggcatcagg
    12481 gcgggtcagc gtcatcaccg acgccagttt attcagcgta ttgcccaggg tttgcggaat
    12541 ggcggtggcg gcaacaaagc gatccgggcg catcaccacc agcgaagcat tgtgttgcgc
    12601 gaaccagcta cgcagacgac cttgcgtatc gcccacgcgt agcacgccgt cgtggttatc
    12661 ctgtgcggta tgaatttgca cttccggcac cacctgaatg aagcgtgtgc ccaacgcgcg
    12721 ccactgctgg atttgctcgt cgctcatccc ccacagtgga ttgcatcccc agccaattac
    12781 cgcgaagttc gcgccaatcg cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac
    12841 tttcggctga ataaacatct tgccgaccgg agagtgcttc gcttcgccct cacgtaccag
    12901 cgcaccgccg taatattgcg gcatcggctt gaagcgcatt tcgaggaagt agcgttttac
    12961 tggcggcaga taattcagta gccaggaaac gccgtcacgt aacgtaccct gccagcgttt
    13021 cggcggagcc agcacgttgc ccaccgtcac ggacaggtca atcatcgctt tggcgtgatc
    13081 gcgacgttct tgttgatagg tatcgagcag cgcatcgcgg gctttcccct ggataaccaa
    13141 cgccagtttc caggcgaggt taaaggcgtc gcgcatacca ctgttatagc cctgcccctg
    13201 ccacaccggc atgatgtgcg cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg
    13261 ttgcgccagt cgcgcgttat gggtgtagac acgctggcga atcaattcaa cattgtccgg
    13321 attaggcagc actttgctta acagcttgcg catattttgc ggctcacgca gctgctcttc
    13381 ggtttctccc ggcatcacca taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga
    13441 aacatacggg cgcaccggat cgcaacacaa atagatatgc ggcgtactta acggatcgtt
    13501 ggcgatatct accacaatcc actgatttgg cgcagtttta ccttcaaacg gcacattcag
    13561 agtgcgacgg acaaaacttg ctccaccgtc acaggctacc agccactggg ctttgactat
    13621 ttcccgctgc ccttctgccg ttttcaggtg caaggtcact tcgtcatttt gctggctgaa
    13681 ggcctccagc tcgcgggaaa acaagcagcg cacattagga aaacgcgaca ccccttccag
    13741 catcaccgca tcgacctgcg gctgaataaa ggcgttacgg cgcggccagc caaattcatc
    13801 ggtcattggc tgaatatcag caaaacagcg gcctttcggg gtgagaaaac gcatcgcgtg
    13861 ccacggcgta gtgtgcggca gaacattctc gaccaggcca accgactgca tggtgcgcag
    13921 cgcctcgtca tcaataccaa tcgcacgcgg gtagtcgatc aacttatcga gtttctccac
    13981 caccagcacg tcaatgccca tctgaccgag atagttcgcc atcatcagcc cgaccggacc
    14041 ggcacctgcg atcgccacct gaacgctatg gttaacagca ggctggatgt cagggtgttg
    14101 tattgccatt tcagtacctc acgactcgga caaaatgccg ttgcgcgcac agtacggcgc
    14161 aacttatttt gttaaaaaca tgtaaatgat tttttattgt gcgctcagta taggaagggt
    14221 gttttcggct acaatcaaaa catgcccgaa tgtgcaccag gtgcacaacg ttgttttaac
    14281 tatagaaatg tcaattaata tgcagaacaa tgagcagacg gaatacaaaa ccgtgcgcgg
    14341 cttaacccgc ggtctaatgt tattaaatat gttaaataaa cttgatggcg gtgccagcgt
    14401 cgggctgctg gcggaactca gcggcctgca tcgcaccact gtgcggcgac tgctggagac
    14461 gctgcaggaa gagggatatg tccgccgtag cccctccgat gatagttttc gactgaccat
    14521 caaagtgcgg caattaagcg aaggatttcg tgacgaacag tggatttctg cactggcggc
    14581 cccactgctg ggcgatctgt tgcgcgaagt ggtatggccg acagatgtgt ccacgctgga
    14641 tgttgatgca atggtggtac gcgaaaccac tcaccgtttc agccgcttat cctttcaccg
    14701 ggcaatggtc gggcgacgtt tgccgcttct gaaaaccgcc tcgggcctga cctggctggc
    14761 cttttgcccg gaacaagacc gcaaggaatt aatcgaaatg ttagcctccc gccccggtga
    14821 tgactatcaa ctggcacggg aaccgttaaa gctgcaagcc attctggcgc gcgcgcgcaa
    14881 agagggttac ggacagaact accgcggctg ggatcaggag gagaagattg cctctatcgc
    14941 cgtaccgctg cgcagtgaac aacgggtgat tggctgtctg aatctggtgt atatggcgag
    15001 cgcaatgacc attgaacagg cagcggaaaa gcatcttccg gcgctacaac gggtagcaaa
    15061 acagatcgaa gaaggggttg aatcgcaggc tattctggtg gccggacggc gaagcggcgt
    15121 gcatttacgt tgacaccatc gaatggcgca aaacctttcg tggtatggca tgatagcgcc
    15181 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgctatac gatgtcgcag
    15241 agtatgccgg tgtctcttat cagaccgttt ctcgcgtggt gaaccaggcc agccacgttt
    15301 ctgcgaaaac gcgagaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
    15361 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
    15421 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
    15481 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
    15541 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
    15601 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
    15661 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
    15721 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
    15781 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
    15841 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
    15901 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
    15961 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
    16021 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
    16081 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
    16141 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
    16201 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    16261 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    16321 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtgaaatt
    16381 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc
    16441 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acacaactta atcgccttgc
    16501 ggcacatccc cctttcgcca gctggcgtaa tagcgaagaa gcccgcaccg atcgcccttc
    16561 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc
    16621 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
    16681 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac
    16741 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa
    16801 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc
    16861 ggcgtttcat ctgtggtgca acggtcgctg ggtcggttac ggtcaggaca gtcgtttgcc
    16921 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt
    16981 gctgcgctgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat
    17041 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc
    17101 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg
    17161 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc
    17221 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtagtt atgccgatcg
    17281 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct
    17341 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg
    17401 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc
    17461 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga
    17521 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg
    17581 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta
    17641 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga
    17701 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg
    17761 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca
    17821 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg
    17881 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga
    17941 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg
    18001 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg
    18061 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg
    18121 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta
    18181 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt
    18241 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca
    18301 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga
    18361 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt
    18421 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc
    18481 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg
    18541 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt
    18601 gacgctcccc tccgcgtccc atatcatccc gcaactaacc accagcgaaa cggatttttg
    18661 catcgagctg ggtaataagc gttggcaatt taaccgtcag tcaggccttc tttcacagat
    18721 gtggattggc gatgaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc
    18781 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga
    18841 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gctcggcaga
    18901 tacacttgcc gacgcggtgc tgattacgac cgctcacgcg tggcagcatc agggaaaaac
    18961 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt
    19021 tgatgttgaa gtagcgagcg atacaccgca tccggcacgg attggcctga cctgccagct
    19081 ggcgcaggtc gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga
    19141 ccgccttact gcggcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc
    19201 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc
    19261 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat
    19321 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg
    19381 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtgtcgg cggaattcca
    19441 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg
    19501 caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac
    19561 aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt
    19621 cccgtttttc ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat
    19681 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc
    19741 tgacaaactc gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt
    19801 tgcgccgttc tttattttta tcttcgggcc actgttacaa tacaacattt tagtaggatc
    19861 gattgttggt ggtatttatc taggcttttg ttttaacgcc ggtgcgccag cagtagaggc
    19921 atttattgag aaagtcagcc gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg
    19981 ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcaccg tcaataatca
    20041 gtttgttttc tggctgggtt ctggctgtgc actcatcctc gccgttttac tctttttcgc
    20101 caaaacggat gcgccctctt ccgccacggt tgccaatgcg gtaggtgcca accattcggc
    20161 atttagcctt aagctggcgc tggaactgtt cagacagcca aaactgtggt ttttgtcact
    20221 gtatgttatt ggcgtttcct gcacctacga tgtttttgac caacagtttg ctaatttctt
    20281 tacttcgttc tttgctaccg gtgaacaggg tacgcgggta tttggctacg taacgacaat
    20341 gggcgaatta cttaacgcct cgattatgtt ctttgcgcca ctgattatta atcgcatcgg
    20401 tgggaaaaat gccctgctgc tggctggcac tattatgtct atacgtatta ttggctcatc
    20461 gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc
    20521 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc
    20581 gacgatttat ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt
    20641 actggcgggc aatatgtatg aaagcatcgg tttccagggc gcttatctgg tgctgggtct
    20701 ggtggcgctg ggcttcacct taatttccgt gttcacgctt agcggccccg gcccgctttc
    20761 cctgctgcgt cgtcaggtga atgaagtcgc ttaagcaatc aatgtcggat gcggcactgg
    20821 gcacatccga caattattta tagctccatc atctggcgaa acaagcgtcg catcatacct
    20881 ctttgaccca cagctgcgga aaacgtgctg gtgcaaaacg cagggttatg atcatcagcc
    20941 caacgacgca cagcgcatga aacgcccagt ccatcaggta attgccgctg atactacgca
    21001 gcacgccaga aaaccacggg gcaagcccgg cgatgataaa accgatcccc tgcataaacg
    21061 ccaccagctt gccagcaata gtcggttgcg cagagtgatc gagcgccagc agcaaacaga
    21121 gcggaaacgc gccgcccaga cctaacccac acaccatcgc ccacgatacc ggcaattgca
    21181 gcggcaacca gataaagccg cagaacccca ccagttgtaa caccagcgcc agcattaaca
    21241 gtttgcgccg atcctgatgg cgagccatag caggcatcag caaagctcct gcggcttgcc
    21301 caagcgtcat caatgccagt aaggaaccgc tgtactgcgc gctggcacca atctcaatat
    21361 agaaagcggg taaccaggca atcaggctgg cgtaaccgcc gttaatcaga ccgaagtaaa
    21421 cacccagcgt ccacgcgcgg ggagtgaata ccacgcgaac cggagtggtt gttgtcttgt
    21481 gggaagaggc gacctcgcgg gcgctttgcc accaccaggc aaagagcgca acaacggcag
    21541 gcagcgccca ccaggcgagt gtttgatacc aggtttcgct atgttgaact aaccagggcg
    21601 ttatggcggc accaagtcca ccgccgccca tcagagccgc agaccacagc cccatcacca
    21661 gtggcgtgcg ttgctgaaac cgccgtttaa tcactgaagg catcaccacc tgaatgatac
    21721 cgatccccac cccaccaagc agtgcgctgc taagcagcag cacactttgg gggtaaagct
    21781 cacgcatcaa tgcaccgacg gcaatcagca acagactgat ggcgacactg cgacgttcgc
    21841 tgacatgctg atgaagccag cttccggcca gcgccagccc gcccatggtg accaccggaa
    21901 gagcggtcaa cagggcagcc acgctaaagc tcattccgct cgcctggcgc aattgcggta
    21961 gcagtggccc gacggaggtg agcagtggtc gcatattaag accaatcagc accagaacca
    22021 gcagcatcgt gaacgtcttt tgatgattgc gcatggctgt cagaacggct tggtcggcag
    22081 atatttacca tctaaggtaa tgaccgcacg ttcgccacct tccgggtccg ccactttctt
    22141 aacgtcgagt ttgaagttaa tcgcgctgat aatgccatcg ccaaacttct catgaaccaa
    22201 cgctttcagg gttgtgccgt acacctgcaa catttcatag aaacgataca tcgttgggtc
    22261 ggttggaata cggtcatcaa tgcagccacg cagtggaatc atctgcaaca gtagaatggc
    22321 gtcttcgtcg agatccagct tcgccccgac caggcgggcg gcgtcggcag gaagcgcttg
    22381 ctgccccagc aaagccgcgg ttacgaaggc ttctgccaga ccggtaccgt cggcaatctc
    22441 ggcaaatgag agatcttttt tagctttgct gagcaaaatg gcatcggcaa gatcaagacg
    22501 aatattgcgg ttaatttgtg actgaatcat ggtggatctc ctgatggttt aaaaataagg
    22561 ttacgctgcg gtcggttggc gtagcggtat ggcacaaacg cgaggattag cggccagtgg
    22621 cacaaactgg cgggttgcgc cgtcaaaagc tgcgatgctg ccgctttcaa tgtcgtagac
    22681 ccagccgtgc agggcgatcc gcccctcttc gagcgccagg cgcaccgatg gatgagtttg
    22741 caaattagcc aactgagcaa tgacgttttc acgtaccatc gccgcagctt ttgacggtaa
    22801 atcggaatgc gggcgcgcct cattaacgac gcgggctgaa tcggcataac gtagccagtg
    22861 ggagacggca ggcatatggt ccatgcactg acagctggca atggcggtca tcgcgccaca
    22921 gttggaatga ccacaaatca caatgtcaga tacccgaagc gcagcgacgg catactccac
    22981 cgaagcagaa acgccaccgg gttccggccc gtaggaaggg acgatattgc ccgcgttgcg
    23041 aataacgaac agatcgccag gctcacgttg cgtcaccagc tcagggacca gacggctgtc
    23101 ggagcaggag ataaaaagtg tgcgcgggct ttgctgtgtc gccagctgtt taaacaaggc
    23161 ttcccgcttc ggaaatgcct cgcgctggaa tttaaggaat ccatcaataa tctctttcac
    23221 cgttaacctc tgtctgtctc tggaatgaga ggcagactac tcgccgattg tcataaggta
    23281 aaagtctcat ttatgatgag ttctattgga tttacttata ggttggcgaa tgctctctcg
    23341 acatatcaat tattttcttg ccgtggctga acatggcagc ttcacccgtg ccgccagtgc
    23401 gttgcacgtc tctcagcctg cgctttccca gcagattcgc cagttagagg agagtttagg
    23461 cgtgccgctg tttgaccgta gcgggcgaac gattcgtctc accgatgcag gagaagtctg
    23521 gcgacagtac gccagccggg cgttacagga actgggagcg ggtaaacggg cgattcatga
    23581 tgttgccgat ctgacgcgag gatcgctgcg tatcgccgtc acccccacct ttacaagcta
    23641 ctttatcggc cctttaatgg cggatttcta tgcgcgctac cccggcatca cgctccagct
    23701 acaggaaatg tcgcaggaga aaatcgaaga tctgctttgt cgcgacgagc tggacgttgg
    23761 gattgccttc gcgcctgtgc attcgccgga gctggaggca attcctttac tgacagaaag
    23821 tttagcgtta gtcgtggcgc aacatcatcc gctggctgcc tgtgaacagg tggcgttgag
    23881 tcgcttgcat gatgaaaaac tggtcctgct cagcgcggaa tttgccacca gagagcaaat
    23941 tgaccactac tgcgagaaag cggggctaca tccacaggtg gtcattgagg cgaactcaat
    24001 tagcgcggtt ctggagctga ttcgccgcac ttccctttcc acattgttac cagcagcgat
    24061 tgccacacaa catgacgggc ttaaagcgat ttctcttgcc ccgccactac tggagagaac
    24121 ggcggttttg ttgcggcgga aaaatagctg gcagacagcc gccgcgaagg catttttgca
    24181 catggcgttg gaggaatgtg cggatgttgg cgagaatgaa tcacggtagc cactgacaca
    24241 ggtcagtggc tcttcaacac agaggaaatt accgtttgta atcgatggct tctggctgct
    24301 ccagatatac ggtggtttgt gccggttgtg tactggcaat caccttgccg ccacgtaccg
    24361 aataacgtac cggaacctga cggcgcagcg catcaaatcc attttcagcc ggcaggataa
    24421 tcaggtttgc gctgtttccg gcggcaatgc cgtaatcctg caaattcaat gttctggcac
    24481 tgtggtgggt gattaaattc aggccatcgt taatctgccc gtagcccatc aactggcaaa
    24541 catgcagccc catatgcagc acttgcagca tattcgccgt tcccagcgga taccacggat
    24601 cgaagacatc atcgtgacca aagcagatgt taatgccgga ctccagcatc tctttaacgc
    24661 gcgtgatgcc gcgacgcttt ggatacgtat cgaaacgtcc ttgcagatga atattgacca
    24721 gcgggttggc gacaaagtta ataccggaca ttttcagcaa gcggaacagg cgtgaggtat
    24781 acgccccgtt ataggagtgc attgccgtgg tgtggctggc ggtgactcgc gcgcccatgc
    24841 cttcacggtg cgccagggca gcaacggttt cgacaaagcg cgactgctcg tcatcgatct
    24901 catcacagtg aacgtcgatg agacggtcgt atttttgcgc cagggcgaag gttttatgca
    24961 gcgactccac gccgtattca cgggtaaatt caaaatgcgg aatcgccccc actacatctg
    25021 cccctaagcg taacgcctct tccagcaacg cttcaccgtt gggatacgac aaaatccctt
    25081 cctgagggaa ggcgacaatt tgcagatcaa tccacggcgc gacttcctgc ttcacttcca
    25141 gcattgcttt cagcgcagtt agcgttgcat ccgaaacatc gacatgggta cgcacatgct
    25201 gaatgccgtt ggcaatctgc catttcagcg tttgccaggc gcgttgtttc acatcgtcat
    25261 gggttaataa cgctttgcgc tcggcccagc gttcaatgcc ttcaaacagc gtgccggact
    25321 gattccagtt cggttgtccg gcggtttgcg tggtgtccag gtgaatatgc ggctccacaa
    25381 acggcggtat aactaaacct tgttcggcat ccaggctgtt ttcagttatg ggcatcacgc
    25441 cggattgcgc atcaatggcg ctgatttttc cgtcctgcag atgaatctgc cacagcccct
    25501 ctttgcctgg taaccgggcg ttaataattg tttgtaaagc gttattcgac actgttaacc
    25561 tccacatgcg tcattgctgc tgtcgtttta cgattcaaaa tcgggttaag gatcagatag
    25621 ctcagcgcgc cacctaatac cgcgttgacc ggaacaattc ccggtaacca gtggcctgcg
    25681 gcaatcccca gcgcgaccgc cagaatcgcc acccaattga cactcatcat acgcgtggtc
    25741 gcaaagtgct catagcggcg acggttcatc agatagtcgg cgatgatcac gccccctacc
    25801 ggaggaatag ctgccgaaag gaaggtcaac cagccgacaa aattgttata cagccataat
    25861 gcgcagaccg taccgataat accgttgatt accgaaaggg ttttgctcga cataccggta
    25921 atgttggcga aacctaaacc cgacgcatag agtgcgttat cgttggtggt ccagatattc
    25981 agccccagca ccacaatcgc gggcagcagc agtccctgag caatcatcac atcagagata
    26041 tctgccatcc ccagtgccgc agcgcccgct gcaccgaaaa taaacatcaa cgagttgccg
    26101 aggaaaaagg ccaccatcgc caccagcacc gccagtttgg cattgcgacc aaaccggaca
    26161 aagtcagcgg tgagcgtacc cgcactgata aatgacccca caaccagcgc cagcgcgaca
    26221 ttgaaatcta acggttgtgc gggaacgacc gcttttaagg cgtccaggcc gcccatgccg
    26281 ttaacggcca gccacacgga ataaccgcca aggcaggcaa tagccggaac cgcaatcacc
    26341 gaaagaaccg tcagcgccga aatgccaaag aagacggtga cggtcatcag taaaccggaa
    26401 acggcaatca gcaaattaat atccagcccg gttgccttgc ccaccggaat agcaaacatc
    26461 gccacgccaa cgccaaacca gccaacctga gttccgccca gtagcagtga aggcagccat
    26521 gagcctttaa caccaaacga gaagcgagca agaagatgag tggtcaggcc ggtttttgcg
    26581 ccaatgtaac cgagaaatga agtgtaaata ccgaggagaa gattaccgat gagaactgcg
    26641 aggaagaaat catgatagct aagaccggtt ccgagagtgc cgccggtcca catactggcg
    26701 gaaaagaagg ttaatcccag catgacgaac gtcaatgcca ataccccttt ccgcgccgac
    26761 tgcgggactg gcccctggct aaagttgtta tcttgcgaca cgaaattccc cctttttgct
    26821 gtttgaaacc caaaaaatcc gccgcattct attcatctga agataaaaag caatcgtttt
    26881 cgtggggaaa tatatttttt atatgaatga gtgcaaaaag gtgggggaaa aattatcggt
    26941 gtaaaaacag tgggtaggca taataagacg cggtaagcgt cgcatcaggc atcggcgcat
    27001 ggtgtcggat gcgccacgcg acgcatccga caatacctta ctcttccatc gcctggcgga
    27061 tctgatccaa cgacgctgga tcatcaatgg tcgtcagatc tccaggatcg cgtccttcgc
    27121 aaatcgcctg gatcgtgcgg cgcagcattt ttccggatcg cgtttttggc aattgcgaga
    27181 caaaccagac gtgcgccggg cggccaaagt tgccaatctg gctgtccacc agcgccataa
    27241 tcgccttctc ttgcgaatgc gccacatcac gatcttccag actgtcgctc tctttcggaa
    27301 tgacaaacgc taccgccacc tgccctttca gcgcatcttt caccccaacc accgccactt
    27361 cggcaacgcc cggatgactg gaaatactct cttcaatctc gcgcgtgccc agccgatgcc
    27421 cggcaacgtt aatcacatcg tcagtgcgcc cgagaataaa gtgataaccg tcagcatcgc
    27481 ggatgcccca gtcaaaggtg gcgtacaccg gacgggaaaa cagcgaccag tacgtcttca
    27541 caaagcggtc gtcatcgccc cagatggtct gaatacaccc cggcggcagc ggcccttcca
    27601 ccaccagcat ccctttctcg ttgacgccac acggttcgcc ggtgacttca ttaagcaact
    27661 gcacgttata gccatacatc ggcacgccgg ggcttcccag acgcgtcggc ctgtcgtcca
    27721 gaccgcgagc aatcgccata atcggccagc cggattcggt ctgccagtag ttgtcgatga
    27781 ccggcacatc cagcgtattg ctcacccaac tggcggtcgg ctcgtccagc ggttctccag
    27841 ccagatagag cacttccagc gacgagagat cgtgtttgcg aatttcagcg gtagggaatt
    27901 ttttcagcac gcgaatggcg gtcggcgctg agaacatccg gctaacctga tatttctcga
    27961 cgattttcca ccacacgccg cagtccggcc aggtcggcaa tccttcgtaa acgatagtcg
    28021 ccatccccgc cagcagcggt gcgtaaacga tatacgaatg ccccaccacc cagccgatat
    28081 ccgatgcgca aaagaacacg ctgcccgctt tgccgccaaa aatggtgtcc atcgaggtcg
    28141 ccagcgccac cgcatatccg ccgacgtcac gctgcacgcc tttaggtttg ccggtcgtgc
    28201 cggaagtgta gagaatgcag gaggtttcgt tggattccag ccacgccacc ggtacccgcg
    28261 cgccgatgtg ttgatggcgc aacgacgcga aatcgacatc ccgcccgctg acgcgcgcca
    28321 ttttcgccag cccgcgatcc accagcaaaa catggcgtgg ctggtgctgc gcctgactta
    28381 tcgcatcgtc gagcaatttt ttatagggaa tgattttgcc accgcgcgct ccggcatcag
    28441 ccgagacaat cagcaccggt ttagcgtcat caattcgcgc cgccacgctg tgcgaggcaa
    28501 aaccaccaaa taccaccgaa tgaatagcac caatgcgcgc gcaggccagc agagtaatat
    28561 gcgcttcggc aatcatcggc atatacacca gcacccgatc gccgcgctgc acgcccagtg
    28621 agcgcaacat tgaggccacc gcgttcactt cgtcatgcag ctggcgaaag gtaaaggtgc
    28681 gctcttcttc tgtttccgaa gagacggcaa tcaatgccag tgcctctggc tgtttctcca
    28741 gccagcggtc gatagcgttg tggcacaaat tggttcggcc ttcacaaaac caacgggcaa
    28801 acggcggatt gctgtggtcg agcgtttgcg taaagggcgt ctgccagtca atacaccggg
    28861 cctgctcggc ccagaactgc tccggttcgt taatcgaacg ctgataaaat tcgctaaaag
    28921 acataatgct ctcctgttga acttacgcct taccgccgtt tacttaaatg acgtacaggt
    28981 cgagatactc attgaccggc atctgttcca ggcgagttct gtcgagagaa acctccagaa
    29041 tgcgctgctg ttggcgagtc gggaactggc gcgcgagatt gattttgaat ttatcgacca
    29101 gtttcggaat accatcctga cggcggcgag catgaccaat ggggtactcc accaccactt
    29161 cttcaaatcg tgtgccgtcg gtgaactcaa gggtaatggc attggcgatg gcgcgttttt
    29221 ccgggtcgtg gtagtcagcg gtaaatgccg gatcttcaaa gcaattgatc ttctcgcgca
    29281 gggcgtcgat gcgtttatct tgcgcaacgt tgtcctcgta atctgccgcc gttaagcgtc
    29341 cgaatagcag cgggatcgcc accatgtact gaatgcagtg atcgcggtct gccgggttat
    29401 tgagcggccc ttttttgtcg atgatgcgaa tacaggcttc gtgggtgcga atggtcactt
    29461 tttcgatatc cgccgccgtt ttgcctgctg cctgcatctg ttcatagagc gtcatcgccg
    29521 cttcaactgc cgtctgggag tggaactccg ccgggaagga gattttgaac agcacattct
    29581 ccatgacgta agaaccgtac ggacgctgga agcggaacga ttcaccttta aaggagacgt
    29641 cgtagaagcc ccataccggc gcggtcaggg ctgacggata acccatttcg cccgttttcg
    29701 ccatcagtgc cagacgtacc gcgcgggaag tggcatcgcc cgccgcccag gatttacgcg
    29761 tgccggtgtt cggcgcatgg cgataggtgc gcagcgactg accgtccacc cacgccagcg
    29821 aaacagcgtt gagaatttcc tcgcgggtca ggccgagcat ttcggcgacc acggcggtgg
    29881 aagccacttt aactaacaga acgtggtcga ggccaacgcg gttaaaggag ttttccagcg
    29941 cgatgcagcc ctgaatttca tgggctttga tcattgcggt cagcacctgt ttcatggtca
    30001 acggcgcttt gccgctggcg accgcgttgc gcgaaagcca gtccgccgtt gccagaatgc
    30061 cgccgaggtt gtcggaagga tggccccact ccgccgccag ccaggtatcg ttgaaatcga
    30121 gccagcggat catcgcgccg atgttaaatg ccgcctggac ggggtcgagc tggaactgag
    30181 ttccggggac gcgcacgccg ttaggtacga cagtgccggg gacaattggc cccagcagtt
    30241 ttttacaggc cgggtattcg agagcttcca gaccgcagcc gagcgtgtca agcaggcagt
    30301 aatgtgcggt gtcgtaggct actttagagc tgatttcgta gttcatgacg taatcgacga
    30361 tatcaacgat ttcacgatca aattccgggc ggatgttgtt gatttgagct gacattgggt
    30421 acgtttcctt attgttattc gtagaggttt actggcgctt atccagcgcg acaaacgggc
    30481 ggtcttccgg tccaacataa ttggcggaag gacggataat tttgttgtcc tgacgttgtt
    30541 caatgatgtg cgccgcccag ccggtgacac gggcgatgac aaacagtggt gtgaacatct
    30601 cggtgggaac gcccatcatg ttgtaggaaa cagcagagaa ccagtcgaga ttggggaaca
    30661 tctttttgct ctcccacatc accgtttcca ggcgatcggc gatgttgtac atcttcaacg
    30721 agccgccttc ctgcgagagc tgcttcgcca cacgtttgat cacctggtgg cgcgggtcgg
    30781 cgatggtata aaccggatga ccaaaaccaa tgaccacttc tttgttttct acccgcttgc
    30841 ggatatcggc ttcggcttcg tccggcgttt cgtagcgttg ctggatctcc agcgacactt
    30901 cattcgctcc gccgtgtttc ggcccgcgca gtgcgccaat cgcgccaata atcgcggaat
    30961 acatatcaga gccagtgccc gcaatcaccc gactggtaaa ggtggaggcg ttaaactcgt
    31021 gttcggcgta cagcactagc gaaatatgca tcgccttttc ccagctttgc gatggctttt
    31081 cgccgtgcag cagatgcagg aagtgaccac cgatggagtc gtcatcggtt tccggctgga
    31141 tgcgttcgcc gttgtgactg tagtgatacc aataaaggag aatcgagcta agtgatgcca
    31201 gcagtttgtc ggcaatatcc cgcgcgccag agacagtgtg cccctctttt tctggcagtg
    31261 tgcagccgag cgcggaaacg ccggtgcgca tgacatccat cgggtgcgac gccgccggta
    31321 aggcttccag cacggtacgc acgttagccg gtaaaccacg cagggctttc agtttcgttt
    31381 tgtaagcggc gagttcgtca cgggtcggca gtttgccgtg gatcagcaga tgagccactt
    31441 cttcaaattc gcaatgttcc gccagatcaa gaatatcgta gccacggtaa tgcaggtcat
    31501 tgccactttt acccacggtg cagagcgccg tattgcccgc cggaacgccg gaaagtgcca
    31561 ccgatttttt cggtttaatg acatgggtac tgttttgcag gatcgttgtg tcgctcatat
    31621 tgtcctcgtc atttgtaggg tcgagatatt tttcgttgtg ttttatttca cctggctacg
    31681 ggcaaacagg tcgtcgagct tctcttcgta ctggtagtag ttgatgcttt cgtacagttc
    31741 gttgcgggtc tgcatggtgt cgatgacgct tttctgcgtg ccttcctggc gcaggacgtt
    31801 gtagacatgt tcagcggcgc ggttcatggc gcggaacgct gaaagcgggt acagcgccat
    31861 tgcgacatgg gcgctgcgta attcgtcggt ggtaaacagc ggcgtggcac caaattcggt
    31921 aatgttggcg aggattggca cctgcaccgc atcggcaaac tggcggtaca tggcgagttc
    31981 ggtaatcgcc tccgggaaca acatctcggc acccgcttca acataggcct gtgcacgctc
    32041 gatcgccgca tccagcccct ctaccgccag cgcatcggtg cgcgccatga tcacaaaatc
    32101 aggatcggtt ttcgcatcca ccgccgcgcg gatccgatcc accatctcct ctttcgagac
    32161 gatcgctttg ttcgggcgat gaccgcagcg tttcgcacca acctgatctt caatatgcaa
    32221 tcctgccgca ccggctttaa tcatcgattt caccgtacgc gccacgttaa aggccgaaga
    32281 accaaaaccg atatccgcat ccaccagcag cggcagcgaa caaacgtcgg tgatacggcg
    32341 aatgtcggtc agcacatcat caagggtaga aataccgaga tcgggcagcc ccagcgaacc
    32401 tgccgccacg ccaccgccgg agagataaat tgcctgatat ccggcacgct gcgccaacag
    32461 cgcatgatta gcgttgatgg tgccaacaat ctgcaatgga ttttctttag acagtgcagc
    32521 gcgaaacgct ttacctggag agtgtagaga catagcccat cctttgttat caacttgtta
    32581 cttgcgtt
//
LOCUS       DAMZII010000042        32505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZII010000042 DAMZII010000000
VERSION     DAMZII010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.042"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            218..1591
                     /gene="aroP"
                     /locus_tag="POA77_001952"
     CDS             218..1591
                     /gene="aroP"
                     /locus_tag="POA77_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDI6365396.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1634..2488)
                     /gene="ampE"
                     /locus_tag="POA77_001953"
     CDS             complement(1634..2488)
                     /gene="ampE"
                     /locus_tag="POA77_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDI6365397.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2485..3036)
                     /gene="ampD"
                     /locus_tag="POA77_001954"
     CDS             complement(2485..3036)
                     /gene="ampD"
                     /locus_tag="POA77_001954"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDI6365398.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3124..4017
                     /gene="nadC"
                     /locus_tag="POA77_001955"
     CDS             3124..4017
                     /gene="nadC"
                     /locus_tag="POA77_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDI6365399.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4220..4660
                     /gene="ppdD"
                     /locus_tag="POA77_001956"
     CDS             4220..4660
                     /gene="ppdD"
                     /locus_tag="POA77_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDI6365400.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4670..6055
                     /gene="gspE"
                     /locus_tag="POA77_001957"
     CDS             4670..6055
                     /gene="gspE"
                     /locus_tag="POA77_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDI6365401.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6045..7247
                     /gene="hofC"
                     /locus_tag="POA77_001958"
     CDS             6045..7247
                     /gene="hofC"
                     /locus_tag="POA77_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDI6365402.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTEKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7282..8325)
                     /gene="guaC"
                     /locus_tag="POA77_001959"
     CDS             complement(7282..8325)
                     /gene="guaC"
                     /locus_tag="POA77_001959"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6365403.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8481..8525)
                     /locus_tag="POA77_001960"
     CDS             complement(8481..8525)
                     /locus_tag="POA77_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365404.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8550..9170
                     /gene="coaE"
                     /locus_tag="POA77_001961"
     CDS             8550..9170
                     /gene="coaE"
                     /locus_tag="POA77_001961"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI6365405.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9170..9913
                     /gene="zapD"
                     /locus_tag="POA77_001962"
     CDS             9170..9913
                     /gene="zapD"
                     /locus_tag="POA77_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDI6365406.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLLLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9923..10120
                     /gene="yacG"
                     /locus_tag="POA77_001963"
     CDS             9923..10120
                     /gene="yacG"
                     /locus_tag="POA77_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDI6365407.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10220..10609)
                     /gene="mutT"
                     /locus_tag="POA77_001964"
     CDS             complement(10220..10609)
                     /gene="mutT"
                     /locus_tag="POA77_001964"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDI6365408.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10669..13374)
                     /gene="secA"
                     /locus_tag="POA77_001965"
     CDS             complement(10669..13374)
                     /gene="secA"
                     /locus_tag="POA77_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI6365409.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13436..13948)
                     /gene="secM"
                     /locus_tag="POA77_001966"
     CDS             complement(13436..13948)
                     /gene="secM"
                     /locus_tag="POA77_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDI6365410.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="POA77_001967"
     CDS             complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="POA77_001967"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDI6365411.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="POA77_001968"
     CDS             complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="POA77_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI6365412.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="POA77_001969"
     CDS             complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="POA77_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI6365413.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="POA77_001970"
     CDS             complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="POA77_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDI6365414.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="POA77_001971"
     CDS             complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="POA77_001971"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6365415.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19413..20888)
                     /gene="murC"
                     /locus_tag="POA77_001972"
     CDS             complement(19413..20888)
                     /gene="murC"
                     /locus_tag="POA77_001972"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI6365416.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20942..22009)
                     /gene="murG"
                     /locus_tag="POA77_001973"
     CDS             complement(20942..22009)
                     /gene="murG"
                     /locus_tag="POA77_001973"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6365417.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="POA77_001974"
     CDS             complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="POA77_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI6365418.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23250..24566)
                     /gene="murD"
                     /locus_tag="POA77_001975"
     CDS             complement(23250..24566)
                     /gene="murD"
                     /locus_tag="POA77_001975"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6365419.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="POA77_001976"
     CDS             complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="POA77_001976"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI6365420.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25645..27003)
                     /gene="murF"
                     /locus_tag="POA77_001977"
     CDS             complement(25645..27003)
                     /gene="murF"
                     /locus_tag="POA77_001977"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6365421.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27000..28487)
                     /gene="murE"
                     /locus_tag="POA77_001978"
     CDS             complement(27000..28487)
                     /gene="murE"
                     /locus_tag="POA77_001978"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDI6365422.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="POA77_001979"
     CDS             complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="POA77_001979"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDI6365423.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="POA77_001980"
     CDS             complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="POA77_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI6365424.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="POA77_001981"
     CDS             complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="POA77_001981"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI6365425.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="POA77_001982"
     CDS             complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="POA77_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI6365426.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7703 a         9142 c         8030 g         7630 t
ORIGIN      
        1 tgagctttac aaacggtttc tttttaagca actcatcttc aaccatgcat aaagcgggtg
       61 cattcgctgc cgcataccat tattcttgat ctgacggaag tctttttgta acaattcaaa
      121 cttctttgat gtaaacaaat taatacaaca aacggaattg caaacttaca cacgcatcac
      181 tgcgtagatc aaaaaaacaa ccaccgcacg aggtttcatg atggaaggtc aacagcacgg
      241 cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc
      301 gatagggacc gggttattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat
      361 tatcctgggt tacgccattg ctggttttat cgcctttctg atcatgcgtc agctgggtga
      421 aatggtggtc gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg
      481 cagttttgcc ggtttcgcct ctggctggaa ctactgggta ctgtacgttt tagttgccat
      541 ggctgagctg actgccgtgg gtaaatacat tcagttctgg tatccggaaa tccccacctg
      601 ggtttctgcc gccgtattct ttgtggtgat taacgccatc aacctgacca acgttaaagt
      661 gtttggcgag atggagttct ggtttgccat tatcaaagtt atcgcggtgg tagcgatgat
      721 catcttcggc ggctggctgc tattcagtgg caacggcggc ccgcaggcga ccgttagcaa
      781 cctgtgggat cagggtggtt tcctgccgca cggcttcacc gggctggtga tgatgatggc
      841 gattatcatg ttctcgttcg gtggtctgga actggtgggg atcaccgcag cagaagctga
      901 taacccggag caaagtatac cgaaagcaac taaccaggtt atctaccgca tcctgatttt
      961 ctatattggt tcgttagccg ttctgctctc actgatgccg tggacccgcg ttaccgccga
     1021 taccagtccg tttgtgctga tcttccacga gttaggcgat acctttgtgg cgaatgcgct
     1081 gaacatcgtg gtactgactg cggcgctctc cgtgtacaac agctgcgtat attgcaacag
     1141 ccgtatgctg tttggtctgg cacaacaggg taatgcgcca aaagcgctgg cgtctgtcga
     1201 taaacgtggt gtaccagtaa ataccattct ggtgtctgca ctggtaacgg cgttgtgcgt
     1261 actgattaac taccttgccc cagagtccgc tttcggactg ttaatggcgc tggtggtatc
     1321 tgcactggta atcaactggg cgatgattag cctggcgcat atgaaattcc gtcgcgccaa
     1381 gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg gtaactggat
     1441 ctgcctgctg tttatggcgg cggtactggt gattatgctg atgaccccag gaatggcgat
     1501 ttcggtatac ctgatcccgg tatggctgat cgtgttaggt atcggctatc tgtttaaaga
     1561 gaaaaccgcc aaagccgtaa aagcgcatta atctctctac gccctcaccc gtacagggtg
     1621 agggcaataa tctttacacc aacgccccgt aaatcgtcag tagtgcaatc accaccacga
     1681 ccacgaacga ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc accttatcga
     1741 catgcggttc acgcgccaga gagaactgcg ccagacgcgt taacacctga tactgcgaag
     1801 tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc tttctcacca tgaccgatca
     1861 aggcatatac cacacccgca agacgaaccg gcacccaatc cagtacatga agcacggcat
     1921 caatgccgga ctgtaaacga tgatgcggcg tctgatatcg cgccagccag tattgccatg
     1981 cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcagcc
     2041 agaacagcgg tgcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac
     2101 gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag
     2161 ccatcgtggc acgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat
     2221 gaagacgaac tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagtagcg
     2281 tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg
     2341 caataatggt catgcctaac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg
     2401 cttcaagacg atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta
     2461 acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg ctgaccagca cacgaaaccg
     2521 tgcccaatca aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc
     2581 cgtcatgttt ttagcgatat ccggatagca atcaatcagt gcccgcgtaa ccgccgcaag
     2641 ctgttgatac tgcgcatcgg tatacgccag cgtatcggtg ccttcaagct caatcccaat
     2701 agaaaaatca ttgcagcgtt cgcgcccctg atactgagag actcccgcat gccatgcacg
     2761 tttatcgaaa ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga
     2821 gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt
     2881 gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac
     2941 caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg
     3001 gcgcgcgcca accagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc
     3061 taatacccgg ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt
     3121 aacatgccgc ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt
     3181 aatctcgata tccccggcgc ggtggcccag gcgctgcggg aagatttagg cggaacagtc
     3241 gatgccaaca atgatattac ggcaaaactt ttaccggaaa attctcgctc tcatgccacg
     3301 gtgatcaccc gcgagaatgg cgtcttttgc ggcaaacgct gggttgaaga ggtgtttatt
     3361 caactggcag gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaat
     3421 gccaatcaat ccttgttcga acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc
     3481 actgcgctta attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc
     3541 gaattgctgg aaggcaccaa cacgcagttg ttggatacgc gcaaaacctt acccggcctg
     3601 cgttcagctc tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt
     3661 tctgatgcct tcctgatcaa agaaaaccat attattgcct ccggctcagt gcgccaggcg
     3721 gtcgaaaaag cgtcctggct gcacccggat gcgccagtag aagtcgaagt agagaatctg
     3781 gaagaacttg atgaagccct gaaagcagga gccgatatca tcatgctgga taacttcgaa
     3841 acagaacaga tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct
     3901 ggcaacgtca ctgacaaaac actgcgtgaa tttgccgaaa cgggcgtgga ctttatctcc
     3961 gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt caatgcgttt tcgctaatag
     4021 ttgacagatt tatccgctcc atcgcgggcg gataatctcc ccttccccaa ctttcttcgt
     4081 aacgcctcgc aaatttcttg tctttcgctg caacgaaata aaaaacctcg gaatctgctt
     4141 tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt ttgccaaagt agcgccaact
     4201 aaatcaagga gcgaaacaga tggacaagca acgcggtttt acacttatcg aactgatggt
     4261 ggttattggc atcattgcca ttttaagcgc cattggtatt cccgcttatc aaaactacct
     4321 gcgcaaagcc gcactcaccg acatgctaca aacctttgtg ccttaccgta ccgccgtaga
     4381 gttgtgcgcg ctggaacatg gtggattaga tacctgcgac ggtggcagca atggcattcc
     4441 ctcgcctacc accacccgct atgtttcagc catgagtgtg gcaaagggcg tggtgtcgct
     4501 gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg acaccgggtt gggataacgc
     4561 aaacggcgtc accggctgga cgcgcaactg caatattcaa agtgacagcg cattgcatca
     4621 agcctgcgaa gatgtcttcc gctttgatga cgccaactaa ggagcggcaa tgaatattcc
     4681 acagctcact gccctgtgtc tgcgttatca tggagtcttg ctggatgcca gcgaagaggt
     4741 ggttcatgtt gcggtagtcg atgcaccttc gcatgagcta ctggacgcat tgcatttcgc
     4801 taccaccaaa cgtattgaga tcacctgctg gacgcgccaa caaatggaag gtcacgccag
     4861 tcgcacacaa cagacattgc ccgtagctgt tcaggagaag catcagccca aagcagagtt
     4921 gctaactcga acgttacaat ctgcgctgga acaacgcgcg tctgatattc atatcgaacc
     4981 agcggacaat gcctaccgca tccgcttgcg tatcgacggc gtattgcatc ctttaccgga
     5041 tgtttcaccg gatgccggag tcgcattaac cgccagatta aaagtgctgg gaaacctgga
     5101 tattgcggaa catcgcctgc cgcaggacgg gcaattcact gtcgaactgg caggaaacgc
     5161 cgtctcattt cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt
     5221 gttacagcag gtgagccagg cactggatgt caacacgctt ggaatgcagc cgttacaact
     5281 ggcggacttt gctcatgcct tgcaacaacc acagggactg gtgctggtaa ctggccctac
     5341 aggcagcggc aaaacggtca cgctttatag tgccctgcaa acgctgaata ccgctgacat
     5401 taatatttgt agcgtcgaag atccggttga gatccccata gccggactaa accagacgca
     5461 aatccatccg cgtgccggac tcacctttca gggcgttttg cgtgcgttat tgcgccagga
     5521 tcctgacgtc atcatgatcg gagagatccg cgatggcgaa acagcagaga tcgctattaa
     5581 agcggcgcaa actggtcacc tggtgttgtc taccctacac actaattcca cctgcgaaac
     5641 gctggtacgt ttacagcaaa tgggagtcgc ccgctggatg ctctcatcag cgcttacgct
     5701 ggtaatagcc cagcgtctgg tacgtaaact ttgcccacat tgtcgccagc agcaagggga
     5761 gcccatccat attccagtca atgtatggcc gtcgccgctg ccccactggc aggcacccgg
     5821 ttgtgtacat tgctaccacg gtttttatgg tcgtacggcc ttatttgaag ttctgcccat
     5881 aacgccggtc attcgtcagc ttatttccgc taataccgac gttgaatcgc tggaaacgca
     5941 cgcacgacag gcgggtatgt gtacgctttt tgaaaacggc tgcctggccg tggagcaagg
     6001 cttaaccacc tttgaagagt taatccgcgt actggggatg ccgcatggcg agtaagcaac
     6061 tctggcgctg gcatggcata accggcgacg gcaatgcgca agatgggatg ctatgggcag
     6121 agagccgtgc tttgctgctc atggcactac agcaacagat ggttacccca cttagcctga
     6181 agcgaatcgc catcaattct gcgcagtggc gaggagataa aagcgcggaa gtcattcatc
     6241 aactggcgac gctactcaaa gcagggttaa cgctttctga agggctggct ctgctggcgg
     6301 aacagcatcc cagtaagcaa tggcaagcgt tgctgcaatc gctggcgcac gatctcgaac
     6361 agggcattgc tttttccaat gccttattac cctggtcaga ggtatttccg ccgctctatc
     6421 aggcgatgat ccgcacgggt gaactgaccg gtaagctgga tgaatgctgc tttgaactgg
     6481 cgcgtcagca aaaagcccag cgtcagttga ccgaaaaagt gaaatcagcg ttgcgctatc
     6541 ccatcatcat tttagcgatg gcaatcatgg tggttgtggc aatgctgcat tttgttctgc
     6601 cggagtttgc cgctatctat aagaccttca acaccccact accggcacta acgcaaggga
     6661 tcatgacgct ggcagacttt agtggcgaat ggggctggct gctggtgttg ttcggctttc
     6721 tgctggcgat agccaataag ttgctgatgc gccgccctac ctggcttatc gcgcggcaga
     6781 aattgttgtt acgaatcccg attatgggtt cactgatgag gggacaaaaa ctcacgcaga
     6841 tctttacgat tctggcgctg acacaaagtg caggcattac ttttttacag ggcgtagaga
     6901 gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact tctgacacaa atccagcacg
     6961 atatcagtaa cggtcatccc atctggctgg cgctaaaaaa taccggggag tttagcccgc
     7021 tctgtttgca attagtgaga acaggagagg catccggctc gctggacctc atgttagaca
     7081 acctcgccca tcatcatcgg gataacacaa tggcgctggc ggataacctc gcagccttac
     7141 tggaaccggc gttgctgatc ataacgggag gaattatcgg tacgctggtg gtggcgatgt
     7201 atctgccaat tttccattta ggcgatgcga tgagtgggat gggataacgc tggcgtgttg
     7261 ctccacacca gcgttggaag attacaggtt gttgaagatg cggttttctt gttcctgcac
     7321 acggataaat gtggtgcgct tggtcagttc tttcaggcgt gaagccccaa cgtatgtaca
     7381 agctgaacgc aggccgccca aaatatcacg cgcggtattt tcaaccgggc ctcgcagcgg
     7441 cagcttaacg gttttacctt ctgctgcgcg atattccgca acgccgccaa cgtgacgttt
     7501 catcgcagac tcggagctca tgccgtagaa cagcataaat ttctcgccgt tctcctcaac
     7561 gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg ccaagcatga cgaaatcggc
     7621 accgccgcca aaggcttttg cgacatcgcc cggcgtggtg cagccaccgt cgctgacgat
     7681 cattccgccc agaccgtgcg ccgcatcggc acattcgatt accgcagaaa gctgcggata
     7741 accgacgcct gttttgacgc gagttgtaca aacagaacct gggccaatgc caactttaac
     7801 gatatcagca ccggagagga taagctcctc acacatttca ccagtcacta cgttgccagc
     7861 acaaatggtt ttggtcggcc acgcttcacg cgctttcgca acgaactgca cgaagtgttc
     7921 ggaataacca ttcgccacat caatacaaac aaagtttaat gccgggttca ggtcgagaat
     7981 ctgtttagtt ttttcgaaat ccgcatcaga cgtaccggta gaaaccatca catgtttcag
     8041 cacatcagcg gaagaattgt tgataaacgc ttgccactct tcgacagaat agtgtttatg
     8101 cacagcagtc aaaatatcaa aagaagccag cgcagaggcc atagaaaatg tacctacggt
     8161 gtccatattt gcggcgataa tcggcacgcc ggaccagctc tgacctgaat gtttgaaggt
     8221 gaattgacgt tccagttcaa catcggaacg gcttttaaga gtggagcgtt tagggcggat
     8281 gagaacgtct ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg gattcctggg
     8341 gttaatggct ataagggtaa gagcgcaact ccagtgacgc tatcatacgc actaatcaat
     8401 gcggcgcaag actgcgaaat tgtctttttt tacgctacaa tcccataaat ttacctggta
     8461 aatagtgagc gaacgtttca tcaatttcgc tttcgtgcgc tcatttttaa cgttttgata
     8521 atcatgatga attcccggga atataaatta tgaggtatat agttgcctta acgggaggca
     8581 ttggcagtgg caagagtacc gttgccaatg cgtttgctga tctcggaatt aacgtcattg
     8641 atgccgatat tattgcgcgt caggtggttg aaccaggtgc acctgcgcta catgccattg
     8701 ctgatcactt tggcgaaaac atgattgctg ctgatggaac attgcagcgc cgggccttgc
     8761 gcgagcggat cttcgccaac ccggaagaga aaaactggct taacgacctg ctgcatccgc
     8821 tgattcagca agagacgcaa caccagatcc agcaagcaac ttccccctat gtactgtggg
     8881 ttgtgccatt gctggtagaa aactcactgt ataaaaaagc gaatcgagtg ctggtggtgg
     8941 atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta actcgcgaac
     9001 atgtcgaaca aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc gtggcagatg
     9061 acgtcattga taataacggc gcaccggatg ctatcgcatc ggatgttgcc cgcctgcacg
     9121 cacactattt gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa tgcagaccca
     9181 ggtccttttt gaacatccac taaatgaaaa aatgcgtaca tggctgcgca ttgagttttt
     9241 gattcagcaa ctcaccgtta atttacccat cgttgaccac gctggcgcgc tgcatttctt
     9301 ccgtaatgtc agtgaattac tggatgtttt cgagcgcggc gaagtccgca ctgagctgtt
     9361 gaaagaactt gaccggcagc aacgtaaact ccagacctgg attggcgtgc ctggcgtgga
     9421 ccagagccgt attgaagcat taattcagca gttaaaagcg gcggggagcg tattaatttc
     9481 cgcgccgcgt atcgggcaat ttctgcgtga agatcgtttg attgctctgg tgcgtcagcg
     9541 actgagcatc ccaggcggct gttgcagctt tgatttacct acattgcaca tttggctgca
     9601 tctaccccag gcgcagcgcg acagccaggt agaaacctgg attgccagcc tgaacccgct
     9661 cacccaggca cttaccatgg tgctggattt aattcgccag tcggccccct tccgtaaaca
     9721 aaccagcctg aatggttttt atcaggataa cggtggcgat gccgacttgc tgcgcctgaa
     9781 tctgttgctc gattcacagc tttatccgca aatttccgga cataagagcc gttttgccat
     9841 tcgttttatg ccgctggaca ctgaaaacgg acaggtaccg gaacgtctgg atttcgaact
     9901 ggcctgttgc taaggagtga aaatgtcaga aactattacg gtgaattgcc caacctgcgg
     9961 gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca ttttgctcca aacgttgtca
    10021 gctgatcgac ctcggagaat gggctgctga agaaaaacga atccccagca gtggcgatct
    10081 ttccgaaagc gatgactgga gcgaagagcc aaagcagtga catttgtctg atgccgcacg
    10141 taggcctgat aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg
    10201 cgtgaacgcc ttatccgacc tacagacgct taagcttcgc aattaccggt tcattggctg
    10261 gcggaaaatc atcggcatta agaccgacta gcgacatcca ctcaccgggt tgcccttctt
    10321 taccccacgg ctccccttcc cagctttcga ccagccagaa ccacagagtt atatgcctgt
    10381 ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgactt
    10441 cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc gcccatttca attttaccgc
    10501 cgggaaactc cagtttattc gccatgtgcg catctgctgc gcgacgcgtt ataaagattt
    10561 cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt gtcttaaacc
    10621 tataaaaaag gcgcagaatc ctgcgccttt tacttcaact gttagctttt attgcaggcg
    10681 accatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac
    10741 tttgcgctca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    10801 gctaagctgc tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    10861 ctcaacctct tcaggcatac gtacctggac tttgctcagc gtgctgataa cttcatattt
    10921 taacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    10981 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgctgccag
    11041 gtgctctttc cacagggagt caagcgtttg cagcatgacg cctttctcga agtgacgcat
    11101 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    11161 gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cggcaattgg
    11221 taaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    11281 ttccagcgac tgtggcggaa tgtaggcatc aatggtggct ttgaacacat cttcacgaat
    11341 gctgttgatg gtttcgctca catcgctgac atccagcagt tcgttacgct gggagtaaat
    11401 ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    11461 gttacggctt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc
    11521 aatggcttcg cctggcttca tacccagttt acgcatcatg ccggatactc ggtcggaagc
    11581 aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc
    11641 ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt
    11701 accgatgata tgcaggccac ctgcttccag taccgcatcg tgacgtacct gccagtcggc
    11761 tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca
    11821 gctaccaccg agcacaatat ctgtaccacg acccgccata ttggtcgcga tagtcaccgc
    11881 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag
    11941 gacgttgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat
    12001 ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    12061 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    12121 cattggacgg ttggtcggaa caacgacggt atccagcttg tagatggagc taaattcgaa
    12181 agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt
    12241 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    12301 ttccacagcc tggtgcagac catcggacca gcgacggccc tgcatggtac gaccggtgtg
    12361 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcgc gggtaaacag
    12421 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    12481 cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag
    12541 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    12601 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    12661 gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc
    12721 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    12781 acgttcttca gggctgaacg ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt
    12841 cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg
    12901 caggttgata ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    12961 acgttgcgcc aggtagtcgt tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt
    13021 caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat
    13081 gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa
    13141 gacgcgctta cttgcctcac gtaccacggc gaaagcttcc gggatcagat tttccagcac
    13201 ttcgcctttt tccagacgtg cacgaaactc tgcggttttc cctttcagtt cttcgtcgga
    13261 gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    13321 cagggtgcga tcgttacgac taccgaaaac tttagttaac aatttgatta gcataataaa
    13381 atctcaaacg ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagg
    13441 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    13501 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    13561 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    13621 gcaaagattc ttcagcaacg ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga
    13681 ttaccgtgcg aatggcatgt tgatgccagt aatcaacgga atagttcgaa ttcgggcggc
    13741 gtgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    13801 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac
    13861 ctaaactcgc cgcaaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    13921 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcatat tcaaacgtat
    13981 ttagcgcaca aatcttatca ttaaagcctg cagtccacag cacgaatgaa gataagttgt
    14041 gcggaaaagt gcttaagaaa acagcgttcg catcgttttt tatagctctt ataaaattca
    14101 aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt cgaattttat
    14161 acgacagtat aaatgtcgtt atgccagtac agctgaaggc gctttgaagg ccaacggcag
    14221 ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgtttcgcca ggacagcctg
    14281 cagcagtttg ttattcagtg catgaccgga tttataagcg gtaaatgcac caataatatt
    14341 gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtgacgca caaattcgtc
    14401 ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc
    14461 gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac gcatgaaacc
    14521 gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt tcatcgcata
    14581 gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa
    14641 accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa cagtctcttt
    14701 gatgcgaaca aattttttgg cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta
    14761 tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa cttcgataac
    14821 aatgttatcg atgcccaagc ccgcgagagc agcattgagg tgctctacgg ttgaaatccg
    14881 tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag atttggcatc
    14941 ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtgttggc
    15001 cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga cacccgtcgc
    15061 ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc aaattaccta
    15121 tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc ctcgaaaccc
    15181 aaattccagt caattcttaa tcagcttgct tacgcaggaa tgctgggata tccagataat
    15241 ccggctcttt cgcagtttgc ggcgcattgt cattcacgac tttagcaacc ggcttctgct
    15301 cctgggtcag cggagccatc ccatgctgct ggtagcgatc catcactggc tgctgaacct
    15361 gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct gtcgcaacaa
    15421 cggttacgcg cagctcgtca ttcatatccg ggtcaagaga agtaccgata accacagtcg
    15481 cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca tccagacgca
    15541 ggtcgaagcc cgccgtgatg ttaaccagca cgccgcgcgc gccagacagg tcgatatctt
    15601 ccagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct tcaccgctcg
    15661 ccacgccaga acccatcatt gcgtagccca tctcagacat tacggtgcgt acgtctgcaa
    15721 agtccacgtt catcaaaccc ggacgagtaa tcagttcagc gataccttgc acagcgcctt
    15781 tcagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg cccagaactt
    15841 tcagcagttt gtcgttcggg atagtgatca gagagtccac atgcttggac agttcagtga
    15901 tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa gttgaaaggc ttagtgacga
    15961 cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactggt gctgcacctg
    16021 taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct tccagcgccg
    16081 cacgcaatgc atcgcgatcc tcatcagccg cattgcggcc aacttctgga ttagcgccag
    16141 cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc gctgttttac
    16201 gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaatg cgctcgcgca
    16261 ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt
    16321 cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct gtcgcctgag
    16381 gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa ctattgagtc
    16441 gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct tcaccgttaa
    16501 gatgtgactc tttcccatag tgaagcaatc ccaccgccgt cgaataatac ggctcctgag
    16561 cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca
    16621 cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccggtt aatacaatgc
    16681 ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac tgcaatatct
    16741 cttcgttgac caggttgagc agctcggtat agcgcggctc gatcacctct gccagggtct
    16801 gacgttgcag actccgcggc ggacgaccac ctacgctcgg cacttccacg ctctcatctt
    16861 ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg
    16921 gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca taaggaatta
    16981 ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat atccattgta ccaccaccga
    17041 tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa taacttgatg
    17101 ccagtccggc aaatatcagt tggtcaactt tcagcccaca acgttcaacc gctttgacga
    17161 tgtttttcgc catatcgttg tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc
    17221 ccgaaagtcc taccggattc ttgatccctt cctgatagtc aatcgcatac tcttgcggga
    17281 tcacatgcag cacacgatgc tcatcgcgca cacgcaccga tttcgcggta tggacgacgt
    17341 tttccacatc ttcttgcgtc acttcttctt cagaaatagg caccatacca atttcattct
    17401 ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga caatctgcca
    17461 tcaattctgc ctggtcaatg gcgcgttgta cgcacttgac cacggattcg aggtcgttca
    17521 ccccgccttt atccatacca cgtgacgggc agctgcccac gccaatgata ttgaccatac
    17581 cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatc tccagtccta
    17641 ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct gattttgttg
    17701 ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag agtcataacg
    17761 caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta aaaccggata
    17821 aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat tgagcttaat
    17881 atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt
    17941 aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca cttcattggc
    18001 gctgccttcc gggccataca gcattggaag cacctgcttg ctggtgcgat ctggcggcac
    18061 gctgaaggtg tttccttccg cgtctaccat gtgttgatca ttccaccgcg caatcggcac
    18121 atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc tcacctgctt
    18181 aatccacggc aggcgttgtt ctatttgcgt ctggatgatg ttgacatcct gggtcataaa
    18241 ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac gcgtgtaatg
    18301 gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt ccatccagcc
    18361 caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga aaaggatccc
    18421 cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt
    18481 cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc aactgcgaga
    18541 agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc atacccggtg
    18601 aggtattggc ttccagcaga taaaactgtc catcgctgtc cagcataacg tcaatacgtc
    18661 cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc aaattggcct
    18721 cttgtgacgc ttccagacct gcggggcaga aatactgtgt ctcatcagag agatacttcg
    18781 cctcataatc atagaaggtt ccggccggtt gaatacgtat tgacggtaaa atttcttcac
    18841 cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat acttcttcat
    18901 cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact acttttgaca
    18961 ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaaccc agagcagaaa
    19021 tttctgctaa ctgcttatcg ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc
    19081 acggcgcgac cggtaaaccg gcaccttgcc acagcagttt gctgcgtagt ttatccattg
    19141 aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc
    19201 cctgcagcgt gccatcttca ccgccgcgac cgtgtagcgc gataaacact ttctgaaagc
    19261 ccatcgactt cagttgcgtc acgtcgactt ctttcgggtc gacaggatac gcatcaatac
    19321 cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact tcccgctcag
    19381 cggaagtccc gcccaacagg accgcgattt tatcagtcat gttgttcttc ctccggagtt
    19441 tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccgatatt accagccccc
    19501 tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc tacctgcgca
    19561 gggtccggca ccagaatggg atcaattttc ccacgtccac gaattgtgcg acacagcgaa
    19621 cggctgtccg ctcccggaat tggcgcttcg ccagccggat acacttccag catcaacagg
    19681 gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa
    19741 cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcgcg cgccgcttta
    19801 atggtggcgt caacttccgt cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg
    19861 cttttaccat tcactggctc cagcgggaat tcaccgagga aatcaaaacg gcgcccagtc
    19921 ccctggaagc tctccagcgc acgtaaaata gcctcgtcat caattccctc ttccgtagca
    19981 accgcaaccg cagctgcggc gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg
    20041 cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga
    20101 taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc
    20161 actcgcggta acaattcgcg gatcaccgga tcatcaacac acatcaccgc acgaccgtaa
    20221 aacggcaggt tgtgcagaaa attaataaaa gtctgtttta aattctcaaa gtcgccctgg
    20281 taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc
    20341 aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg ccccaaacgc
    20401 gcatgaaccc ccgccgcttt taccagcccg ccgttaacga aggttgggtc gagccccgct
    20461 tctgcgtaga tgctggaaac catcgcggtg gttgtcgttt tgccgtgcgt tcctgcaatg
    20521 gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg gatcaccgga
    20581 atacgcgctt catgagctgc gacaatttcc gggttatcgg cagaaatcgc gctggaaaca
    20641 acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat cgtcgcccca
    20701 agattcatta actgttgcgt gaccggattt ggcgctaaat cggaaccact gatctgataa
    20761 ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccgat gccgacaaaa
    20821 tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc caattgttgt
    20881 gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg catcgctaca
    20941 attacgcccg ggcagcccgg ctcacttcat ttgccactcg ctcggtggca tccggaatgg
    21001 atgcagcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac ccggccaggg
    21061 tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct gcgcccgctt
    21121 tttccagcgg tagcgcattc cagtattgct ggcggtcttt atgttgaaac ggcacaaaca
    21181 acgccggtag tcctgccgcg gcgatttcac tcaccgttaa agcaccggag cggcaaacga
    21241 cgacatccgc ccacgcatac gccgccgcca tatcatcaat aaattccgtc actttatgct
    21301 gcggttgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct ttaccgctct
    21361 gatgccagat agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa
    21421 gaatgcgtgc gccctgggaa ccacccacta ccagcacacg aaccggacct tcacgtccag
    21481 ccaaacgttg ctgcggcaac ggcagcgcca gcacatcggt acgcaccggg ttacccacca
    21541 cttccgcatt agggaaagcg cctggaaacg cctgcatcac tttggtggca atcttcgcca
    21601 gccatttatt ggttaagccc gcaataccgt tttgttcatg aagtacaacc ggaatgccta
    21661 acgaccacgc ggccagacca cctggacctg atacatagcc gcccataccg agtaccacgt
    21721 caggtttgta cgctttcata atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg
    21781 gcgcagctat cagtgctttt ataccttttc cacgcagacc agagatacga atgaaatcaa
    21841 tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc cccagccagc
    21901 gaacttgcca accctgagcc attagatggt gcgcaaccgc cagtcccggg aatacatgtc
    21961 caccggttcc gcctgccatc accattaatc gctttccttg accactcatc gtgaacctcg
    22021 tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca gcatcatgat
    22081 ggctgtcgac ataatcagta agctcgaacc accgtaactg atcagcggca atgtcagacc
    22141 tttggtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga agctaaacca
    22201 gatgccaata gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg
    22261 gccaatcgac atcgcgcgaa aagcgacgaa gaataccatt aaaagtgcca gcaccacacc
    22321 gacatacccc agttcttcgc cgataatggc gaaaataaag tcagtgtgcg cttccggcag
    22381 atactccagt ttttgtaccg agttacctaa accttgtccc caaagttcgc cgcgaccaaa
    22441 cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc acgggttcca
    22501 gaatgcggta acacggcgga tacggtacgg ttcggcgagt atcagcaaca caaccgctga
    22561 aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca ggaacaacat
    22621 cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg ccagcagtaa
    22681 cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac
    22741 ttcgtcgcct ttacgcacca gatagttggc gatataacaa aacagcgaca gttttgtcag
    22801 ctccgcaggc tggatacgca gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga
    22861 gctacccact accagcacga tcatcagcag gatgatagat ccgagcagca tcgtggcact
    22921 gtagcgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa tgaatgccag
    22981 gatcagatag acgccatcac gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc
    23041 tatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcaaggc cgaaggtcag
    23101 ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc gcgagcccat
    23161 cacccagcct tttagcgccg tggagatcca gaccaggata ctgaatcctg gcaggcgcgg
    23221 cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag acgggcaaac
    23281 tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc cggggagagc
    23341 agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatcgcctg ttccatagtt
    23401 tcggtttgtt ctgccacttc cgggcgtagc gccgccagct gcgcaccgtc acgaccaaaa
    23461 cagtacaaac gcacgttgtc gccattcaga tagcgcgcca gtggactgaa gtccgccgat
    23521 ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cctgcaagcc attcaacgcc
    23581 gcttccgtac tgccgacgtt agtggctttc gaatcgttaa tccagcgtac gccattgtgc
    23641 tccagcacca cttcaaagcg atgcggcaga ccggtgaagg tggttaacgc tttcaggctg
    23701 ctggcgcgcg gcaaaccagc tgcgtctgcc agtgccagtg ctgccagcgc attggtgtag
    23761 ttatgctgcc cggaaagttt catctctttc acgttcagca ctttctcgcc tttcactcgc
    23821 agccaggttt ctccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg
    23881 acgcagcgtt catcagcacc gcgaatcggc attgttaagg catcatcagc attgacgaca
    23941 caaactttcg cattttcgta aatacgcaat tttgccgcac ggtattgctg caaaccaaac
    24001 ggatagcggt ccatatgatc ttcagtcacg ttcagaatgg tcgccgccac cgcctgtaag
    24061 ctggaagtgg tttccagctg gaagctcgac agttccagca cgtacagttc gcactcagca
    24121 tcaagcagca tcaacgctgg caggccaata ttgccaccca caccaacgtt aacccccgcc
    24181 gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc
    24241 gccacaatcg gtgcttgtgc ttcgcggcag aacagctcga tatcgccaac gatttcgatt
    24301 ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca
    24361 atcagatctg ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct
    24421 tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca
    24481 ccgcgagcga ggaaaaagtc cacgcaggaa agcccagtga ggcccaggcc gataatgacg
    24541 acatttttac cctgataatc agccatgatt aacgtacctt cagcgttgcc agaccaatca
    24601 gaaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt
    24661 tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa
    24721 aggagccgac ctgcaggatg acagaaagcg tttctaccac gaacacgccc cccataatca
    24781 ccagcaggaa ttcctgacgt agcagtacgg cgataatgcc taacgcacca cctaacgcca
    24841 gcgaacctac atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca
    24901 gtcctgcccc gactatcgcc gtgcagacaa tgaccagttc ccctgcgtgt cgcagatacg
    24961 gtatatgcaa gtagctggca aagttcatat tgccggtcgc ccacgccacc agcgcaaaac
    25021 caccggcgac aaatacggtc ggcataattg ccaggccgtc gagaccatcg gtcaggttta
    25081 ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagct
    25141 gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg
    25201 caaggtacag ggcgaaggcg acacccagcg caatgaccga catccagaaa tacttccaac
    25261 gagcgatcaa ccctttggtg tctttacgca ccactttgcg ataatcatca acaaagccaa
    25321 taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag
    25381 cccacagcag tacggagatc actatcgccg tcaggatcat aatcccgccc atggtcggcg
    25441 taccgcgctt gctgaagtgt gattcaggac cgtcgttacg caccacctga ccaaaggaaa
    25501 gtttttgcaa atgagcaatc atacgcgggc ccatccacaa tgagatgaac agcgcggtca
    25561 gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt
    25621 tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac
    25681 ctcttccatg gcggcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc
    25741 aatcagtgat ttaagacgcg taattaacgc agttttatca gcaaaatgtt cgccaacgcc
    25801 gctggcggtg ctgatagcat ggctttgttt acccacgctt aacacgcggt caataccagc
    25861 agcttttgcc gcctcgccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc
    25921 catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct gaacagcggc
    25981 ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag
    26041 ttggatgggg aacagacggc ctggaacagc tttcagattt gccagccccg ctttgatagc
    26101 atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt
    26161 gtgacgcccc ggcaacggca gcagaacatc gacgctaccg gttggggttt gtagggtaaa
    26221 ttccgtaccg tgcgaggtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt
    26281 gggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt
    26341 gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt
    26401 cgctttcgcg acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt
    26461 gaccagcgca gcttccgggc gagtcagact cacagtccag gctatttcgc cctgatggtt
    26521 cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg
    26581 tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa
    26641 aatcgccgcc gtcatctctt taacggaggt tttgccggag gaccccgtca gagcaaccac
    26701 gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc
    26761 cttgacgatt aactgcggca ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc
    26821 gccgccagct ttcgcctggt cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc
    26881 aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat
    26941 atctgcacct tgcagttcac cgttgagaat gtcggtaagt tggctaaggg ttacgctaat
    27001 catgcaatca cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga
    27061 ttgccaacaa tctggtaatc ttcatggcct ttgcccgcga ccagtaccac atcattctct
    27121 ttagcctgca taacggcgca agtcaccgct tcagcacggc cttccatcac tttggcatgt
    27181 ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc
    27241 gggttatcgt ccgtcaccac cgccacgtca gcaaactctt cggcaattgc gcccatcagt
    27301 ggacgcttac ctttatcgcg atcgccacca cagccaaaga cgcaccacag cttgcccgca
    27361 cagtgcaggc gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc
    27421 accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc
    27481 agacgcgcgg cggttttcag cagatcagcc agtggatagc cgagtgccag cagtgtcgcc
    27541 agcgcgagca gcaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca
    27601 tcgccccaac ttgagctaaa gcgaatcgtc gcaccgctgt cgtgatagtt cacgtcgatc
    27661 gcttttaacc agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaacc
    27721 gcgtccggca gttttgccag ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc
    27781 tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca
    27841 ccatgataat caaggtgatc gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat
    27901 gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg
    27961 ccctgctcca ccagccccgc cagctcatgc tgaacatcga ctgcggaacc ggtagtattt
    28021 tctgtcggga tcactttccc aagcagaccg ttaccaacgg tgcccattac cgcgctggtt
    28081 tcgccaagca gttggctcca ctgcgccagc agctgggtag tcgtggtttt gccgttggtg
    28141 cccgttacgc ctacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt
    28201 gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg
    28261 atttcaccgt cggtcgcctc atctttcgcc tctgcaataa tggcagccac accttgcgct
    28321 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    28381 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    28441 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    28501 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    28561 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    28621 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    28681 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    28741 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ctttcacgcc
    28801 gccgccgcct ggtagcgcca cgctttccat catatgcacc acggtgcgaa caatggattc
    28861 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    28921 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    28981 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    29041 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    29101 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    29161 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    29221 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    29281 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    29341 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    29401 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    29461 accggattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    29521 caagcgttca tcaatactca acgccaggtt gtgcgccgcc tggctgtcag tagaagaaat
    29581 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    29641 aagccattta tcgaaactct tctcaacgcc ctcaatcccc tgactatcga cgttagtaaa
    29701 gccgatgagg tgagcagtca cttcgccgga tggataataa cggcgagatt cttcacgcag
    29761 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt ttacctgacg
    29821 cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag
    29881 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    29941 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    30001 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    30061 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    30121 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    30181 aataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    30241 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    30301 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    30361 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    30421 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa
    30481 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    30541 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    30601 ttctgtcact ctgctgatca tgcgttcgtc ctctctgcga tacgcagaac tgaactacgg
    30661 gcacgaggat tctcagccac ctcttcttcg cccggcatta acttgcctaa tgctcgcagc
    30721 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    30781 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    30841 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    30901 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgagtcgcg
    30961 ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg
    31021 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    31081 aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt
    31141 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    31201 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac accaagatcg
    31261 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    31321 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    31381 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    31441 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    31501 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    31561 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    31621 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    31681 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    31741 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    31801 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    31861 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    31921 ttgaccggca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    31981 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    32041 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    32101 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    32161 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    32221 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    32281 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    32341 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    32401 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    32461 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaa
//
LOCUS       DAMZII010000043        32360 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZII010000043 DAMZII010000000
VERSION     DAMZII010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.043"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(160..288)
                     /locus_tag="POA77_001983"
                     /pseudo
     CDS             complement(160..288)
                     /locus_tag="POA77_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(367..461)
                     /locus_tag="POA77_001984"
     tRNA            complement(367..461)
                     /locus_tag="POA77_001984"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Sec,seq:tca)
     gene            754..2136
                     /locus_tag="POA77_001985"
     CDS             754..2136
                     /locus_tag="POA77_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDI6365427.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2146..4464
                     /gene="yicI"
                     /locus_tag="POA77_001986"
     CDS             2146..4464
                     /gene="yicI"
                     /locus_tag="POA77_001986"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDI6365428.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4517..6226)
                     /locus_tag="POA77_001987"
     CDS             complement(4517..6226)
                     /locus_tag="POA77_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI6365429.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6347..7738)
                     /gene="xanP"
                     /locus_tag="POA77_001988"
     CDS             complement(6347..7738)
                     /gene="xanP"
                     /locus_tag="POA77_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDI6365430.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8018..9223
                     /gene="gltS"
                     /locus_tag="POA77_001989"
     CDS             8018..9223
                     /gene="gltS"
                     /locus_tag="POA77_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI6365431.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9226..10092
                     /locus_tag="POA77_001990"
     CDS             9226..10092
                     /locus_tag="POA77_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365432.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10077..12158)
                     /gene="recG"
                     /locus_tag="POA77_001991"
     CDS             complement(10077..12158)
                     /gene="recG"
                     /locus_tag="POA77_001991"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI6365433.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12164..12853)
                     /gene="trmH"
                     /locus_tag="POA77_001992"
     CDS             complement(12164..12853)
                     /gene="trmH"
                     /locus_tag="POA77_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDI6365434.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12860..14968)
                     /gene="spoT"
                     /locus_tag="POA77_001993"
     CDS             complement(12860..14968)
                     /gene="spoT"
                     /locus_tag="POA77_001993"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI6365435.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14987..15262)
                     /gene="rpoZ"
                     /locus_tag="POA77_001994"
     CDS             complement(14987..15262)
                     /gene="rpoZ"
                     /locus_tag="POA77_001994"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI6365436.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15317..15940)
                     /gene="gmk"
                     /locus_tag="POA77_001995"
     CDS             complement(15317..15940)
                     /gene="gmk"
                     /locus_tag="POA77_001995"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI6365437.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16198..17880
                     /gene="ligB"
                     /locus_tag="POA77_001996"
     CDS             16198..17880
                     /gene="ligB"
                     /locus_tag="POA77_001996"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDI6365438.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17877..18494)
                     /locus_tag="POA77_001997"
     CDS             complement(17877..18494)
                     /locus_tag="POA77_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDI6365439.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18786..19610)
                     /gene="dinD"
                     /locus_tag="POA77_001998"
     CDS             complement(18786..19610)
                     /gene="dinD"
                     /locus_tag="POA77_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDI6365440.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19844..20707)
                     /locus_tag="POA77_001999"
     CDS             complement(19844..20707)
                     /locus_tag="POA77_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDI6365441.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20834..21550
                     /gene="rph"
                     /locus_tag="POA77_002000"
     CDS             20834..21550
                     /gene="rph"
                     /locus_tag="POA77_002000"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDI6365442.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21616..22257
                     /gene="pyrE"
                     /locus_tag="POA77_002001"
     CDS             21616..22257
                     /gene="pyrE"
                     /locus_tag="POA77_002001"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI6365443.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22294..22890)
                     /gene="slmA"
                     /locus_tag="POA77_002002"
     CDS             complement(22294..22890)
                     /gene="slmA"
                     /locus_tag="POA77_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDI6365444.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22997..23455)
                     /gene="dut"
                     /locus_tag="POA77_002003"
     CDS             complement(22997..23455)
                     /gene="dut"
                     /locus_tag="POA77_002003"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDI6365445.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23433..24653)
                     /gene="coaBC"
                     /locus_tag="POA77_002004"
     CDS             complement(23433..24653)
                     /gene="coaBC"
                     /locus_tag="POA77_002004"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI6365446.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHLSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24825..25493
                     /gene="radC"
                     /locus_tag="POA77_002005"
     CDS             24825..25493
                     /gene="radC"
                     /locus_tag="POA77_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI6365447.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25710..25946
                     /gene="rpmB"
                     /locus_tag="POA77_002006"
     CDS             25710..25946
                     /gene="rpmB"
                     /locus_tag="POA77_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI6365448.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25967..26134
                     /gene="rpmG"
                     /locus_tag="POA77_002007"
     CDS             25967..26134
                     /gene="rpmG"
                     /locus_tag="POA77_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6365449.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26232..27041
                     /gene="mutM"
                     /locus_tag="POA77_002008"
     CDS             26232..27041
                     /gene="mutM"
                     /locus_tag="POA77_002008"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI6365450.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVVTKHAQRATFYCRQCQK"
     gene            complement(27080..27559)
                     /gene="coaD"
                     /locus_tag="POA77_002009"
     CDS             complement(27080..27559)
                     /gene="coaD"
                     /locus_tag="POA77_002009"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI6365451.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27567..28844)
                     /gene="waaA"
                     /locus_tag="POA77_002010"
     CDS             complement(27567..28844)
                     /gene="waaA"
                     /locus_tag="POA77_002010"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDI6365452.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLKPYLPPKTH"
     gene            29257..30315
                     /gene="rfaQ"
                     /locus_tag="POA77_002011"
     CDS             29257..30315
                     /gene="rfaQ"
                     /locus_tag="POA77_002011"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDI6365453.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30312..31436
                     /locus_tag="POA77_002012"
     CDS             30312..31436
                     /locus_tag="POA77_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6365454.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31429..32226
                     /gene="rfaP"
                     /locus_tag="POA77_002013"
     CDS             31429..32226
                     /gene="rfaP"
                     /locus_tag="POA77_002013"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDI6365455.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7650 a         8028 c         8677 g         8005 t
ORIGIN      
        1 atggcagtct gattattggt gaagtttata gtcagttttt ttcgccagcg gttaaagaaa
       61 tagaatagtc gtataaaaaa gggtgttctg gggcgagtaa aacaacatct tcaccttaat
      121 ggcgatacag ttggctgaga gtcagcaggg ggggcaaatc acctgcaaat aaacacggcg
      181 catactggcg tgaatatcgc ggatgcgttc gaggacagaa gaaccgacag gcggattctt
      241 cagccgctca tcgtccatca caaaacctta gaccaggtat tcctgaagcc gattgaatat
      301 tgagcagtag aaaatatctg gattgacagt attacaaggg gttggagaac gtcaggagag
      361 gcattttggc ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg
      421 gatttgaagt ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg
      481 gaggcggggc gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc
      541 cgcctaatac acgcagcgta ttatcacgaa agcattatag ttcatataaa acctatattt
      601 tacaatgata tatgagcgaa attaacgcta ccacgatcac ataaaacaag aatcataaat
      661 taataaccag atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg
      721 tctcagcacc cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa
      781 gagaaaattg gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg
      841 ttatatatga tgttctttta taccgatatt tttggcattc ctgccggttt tgtcggaacc
      901 atgtttttgg tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc
      961 gatcgaacgc gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca
     1021 ttcgggatcg tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg
     1081 atctatgcag caattactta caccctactt accttacttt ataccgtcgt caatatccct
     1141 tactgcgcat tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc
     1201 tggcgttttg tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg
     1261 gttaatttaa ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt
     1321 tccgtggtgg cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa
     1381 gcaccaccta caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag
     1441 tggcggattg tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg
     1501 gcgatgatgt attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc
     1561 accacttatt gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg
     1621 aaatgtaaag tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg
     1681 atgttctttg ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt
     1741 gtgttgcatc aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac
     1801 ggcgagtggt gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg
     1861 ctcaaactgg ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga
     1921 tatgatgcgg cagaaaaagc gcagaacagc gccacgatta gcatcattat tgcgctattc
     1981 acgattgttc cggcgatctg ttatttgctg agcgcgatta tcgctaaacg ctactactca
     2041 ctcacgacgc acaatctgaa aaccgttatg gaacagctgg ctcagggtaa acgccgttgc
     2101 cagcaacaat tcacctctca agaagtgcag aactaaggaa cggcaatgaa aattagcgat
     2161 ggaaactggt tgattcaacc tggcctcaat ttgattcacc cgcttcaggt gttcgaggtt
     2221 gaacagcagg ataatgaaat ggtggtctat gctgcccccc gtgatgtgcg tgaacgtacc
     2281 tggcagcttg atactccttt atttacgttg cgctttttct ccccacagga aggtattgtc
     2341 ggtgtgcgga ttgagcattt tcagggggcg ctgaataacg gtcctcatta tccgctcaat
     2401 attttgcagg acgtgaaggt cacaatcgaa aacacagaac gttatgctga gtttaaaagt
     2461 ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt cactggattt tctgcgcaac
     2521 ggcgaacgta ttaccggtag tcaggtgaaa aataatggct acgtgcagga cacgaataat
     2581 caacgcaatt atatgtttga gcggcttgat cttggcgttg gcgaaacagt ttacggtctg
     2641 ggagagcgct ttactgccct ggtgcgcaat ggccagacgg tagagacctg gaaccgggac
     2701 ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt tctacatgac taaccgtggt
     2761 tatggggtac tggtcaatca tccccagtgt gtctcttttg aagtgggatc ggagaaagtc
     2821 tccaaagtgc agttcagcgt tgagagtgaa tatctcgaat actttgttat cgacggcccg
     2881 acgccgaaag cggtacttga tcgttatacc cgctttactg gtcgtccggc gctgccgccc
     2941 gcgtggtcct tcggcctgtg gctaaccact tcatttacca ccaactacga cgaagcgacg
     3001 gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc cgctgcatgt tttccacttt
     3061 gactgtttct ggatgaaagc cttccagtgg tgcgattttg agtgggaccc gctgactttc
     3121 cctgacccgg aagggatgat ccgccgcctg aaagcgaaag gactgaaaat ctgcgtctgg
     3181 attaacccct atatcggtca aaaatccccc gtctttaaag agttacaaga gaaaggctat
     3241 ttactcaaac gcccggacgg ttcgctatgg cagtgggata aatggcagcc aggtctggcg
     3301 atttatgact ttaccaatcc ggatgcctgc aaatggtacg ccgacaaact gaaaggtctg
     3361 gtcgcgatgg gcgttgattg ctttaagacc gactttggcg aacgtatccc aactgatgtt
     3421 cagtggtttg acggttccga tccgcagaaa atgcataacc attatgcgta catctacaac
     3481 gaactggtgt ggaacgtgct caaggacacc gttggtgagg aagaagctgt cttgtttgcc
     3541 cgctcggcct ccgtcggtgc gcagaaattc ccggtacact ggggtggcga ttgttacgct
     3601 aactacgaat caatggcgga aagcctgcgc ggtggtttgt ctattggcct ttcaggtttt
     3661 ggcttctgga gccacgatat cggcggcttt gaaaataccg ctccggcgca cgtttacaaa
     3721 cgctggtgcg cgtttggttt gctctccagc catagccgtt tacacggtag caaatcttat
     3781 cgtgtgccgt gggcctacga tgatgagtcc tgtgatgtgg tgcgcttctt cacgcaactg
     3841 aaatgccgca tgatgccgta tctgtatcgt gaagctgcgc gtgcgaacgc gcggggtacg
     3901 ccgatgatgc gggccatgat gatggagttc ccggacgatc cggcttgtga ttaccttgac
     3961 cgtcaataca tgttaggcga caacgtgatg gttgcgccgg tgttcactga agcgggcgat
     4021 gtgcagttct acctgccgga aggtcgctgg acacacctgt ggcacaacga tgagctcgac
     4081 ggtagtcgct ggcataaaca gcagcacagc ttcctgagtc tgcccattta tgtgcgtgat
     4141 aacaccctac tggcgctggg caacaacgat caacgtcccg attacgtgtg gcacgaaggc
     4201 acggcattcc acctctttaa tctgcaagac gggcatgaag ccgtctgtga agtgcccgct
     4261 actgacggat cggtgatctt tactttaaaa gcagcacgta ctggcaacac gattactgtg
     4321 actggtgcgg gcgaggcgaa gaactggacg ctgtgcctgc gcaatgttgt gaaagtaaat
     4381 ggtctgcaag acggttcgca ggctgaaagt gagcaggggc tggtggtgaa gcctcaaggg
     4441 aatgcgctga caattacgtt gtaatctatt gatggaatgc cgggtacgtc tgagtcgtac
     4501 ccggcgagat tgatgtttac cgcaccggct ccgtcgatgt aacttcaccg ctggaaacga
     4561 tgccagcatt catggtttga gtcacttgct gctttgcggc gttcacggca tgcaattgcc
     4621 cgctaaccgt aggtttcaac gggacattgg cctgaatatc gccactggcg gtaagctgaa
     4681 tattgccgtc gccagtcaac ggtaatttag gccatcccca ctgttgcaaa atattcaccg
     4741 gcacaccgcg tccattcagg ctgatatgtg tctgacgttg tggcgtttgt gaaacactgg
     4801 cggtggcttc cagaatgcct ttttcagtaa atgcactcag ttcgctgata ttcaccgtgc
     4861 tgctgttggc ggtcagcgcc agcgacgggc gacgaacatc aacacgattg aatgtcgcgg
     4921 cggcggcatt cagattcgcc gagccactcc agacgcccca tttatgatcg gtaaccagcg
     4981 tcaggttggc accgtaacca tcgagcgcgg tgagctgcca cgggaagtca gggtcgatat
     5041 caatgatcag attgcggctg gcgctaaatc tcttcagttg caggctgttt aaccaaccgg
     5101 gtgtcgtttc catccacaac tgttgccagt ttttcggcaa ggtatattcc agcccggcaa
     5161 ttgccacatc atcaaggatc aacgttttcc cgtcacgcag ccagttccct gacgttctga
     5221 ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc tacgccctgc ggggaaaatt
     5281 ccgcgtttat aatcgggtca aataaatgca gcgaaccata aatgaactcg ctagcgttca
     5341 tcgacagttt gccttcctgt gtctgccagt catctttact gaaggtcatg ttgcgcaagc
     5401 tgagatcgag gtcggtcacc gcccagtccg gaccttgcaa acgagcatcg atcacttcca
     5461 ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc aaagaagtcg gttagcgatt
     5521 tttcgctttg tagacgtata tccgccatgc gcaggttttc cacttgccag ctgccgtcgg
     5581 cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat gtcggcaccc aggttagtca
     5641 gcgtaacgcg atcgttatca atactgcctt caatcagtac attggtggca ggaacatcat
     5701 tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt cgtacccagc actttaccgg
     5761 cttttggtga ccacggaact acgccgccat ttacccgctg cgcgctcagt ttccattcgc
     5821 tattcgggct attaaacgcc atatcacgca gttgcagacg atcggctttg aacggtagcg
     5881 gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag caggatggta tcgacatggc
     5941 gtggttcggt cagttgccga ctgcttagcg caatgtcgac actttttgcc accagggtcg
     6001 cgggctggcc atcacggcca aacgtgacgt tctccagcac gatatgagat ggcgcggaaa
     6061 aacggtgatc catcgccccg aaggccagat gatagtcgct attctcggaa acccatgcgc
     6121 tgatatgttc tgctccccag cgggtttgca gaagaaaata gaggccagcg atcaccacta
     6181 acagagcgat gagaatgtag agaagcagct tcccaataaa tttcatggtc ttccatcccg
     6241 tgaaatgcac ataagggagt tatgcacgat ttacgcgcaa tcctcaaggc gggaatggtg
     6301 aaagaggtga gactgcggcg gtaattatct taccgccgcc agtgaattac tgtttttctg
     6361 gtgggaaaat cagattcagc acgatggcag taataccgcc cgcggcgatc ccggaggaga
     6421 gcaggttttt cagccattca ggggcaaact gcaaaatcag cggctgctga gacacgccca
     6481 gaccaaccgc cagcgacagc gcgataatca gaatcgcccg acggttcagc ggctcacgag
     6541 aaacgatacg cacaccggag gcggcgatgg tgccaaacat tacaagcgtt gcgccgccca
     6601 gaaccggttc tggaatgtgt tgtacaaaac cgctcactgc cgggaacaga cccagcacga
     6661 tcaacatcag cgcgacgaca aaaccgacat agcggctggc aacaccagtc aactggatca
     6721 ctccgttgtt ttgcccgaag caggagttcg ggaaggtgtt aaacaccgcc gaaacaaacg
     6781 agttcaggcc gtttgccagc acgccacctt tcaggcgttt catgtacagc ggaccggaca
     6841 ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc aatggtttcc agcgaagtga
     6901 tcataaagac cagcatcagc ggcagcagca gactccattc aatgccaaga ccgtaataga
     6961 gcggcgttgg caccataatc agttcttgcg tcatcggttc gttgctttct ggcaacatgc
     7021 ccataaacca cgccagcgca tatccggccg ccatcgcaat caccagtgag gccacgcgta
     7081 agtaagggtt acgttgacgg ttaagcagga taattaaggc taagaccacg cctgccagca
     7141 gcagattttt cggtgcgccg aaggtgttat cgctcatggc tgcgtaaccg ccgccaatgg
     7201 acgttaaccc aacctgaatt agcgacaggc cgataatcat caccacaacg ccagaaacca
     7261 gcggcgtaat aatgcggcgc gccagatgca gaacgcggga gatcaccatc tcggtgcaac
     7321 ttgccagcat caacgtgccg aacaaagccg ccatcatggt aggaacatca gcaccaccgg
     7381 ttttcagcgc ggtaccgccc ataatcagcg gggcaacaaa gttgaagctg gtgccctgaa
     7441 tagacaacag cccggagcca accggacccc aggccttaat ttgaataatc gatgccacac
     7501 cggaggcaaa cagcgacata ctaataatgt gttgcgtgtc ttgtgccggt aaacccagcg
     7561 cctggcagat taatagcgct ggcgtgatca ccgcaacgaa catcgccagc agatgctgac
     7621 aggcggcaaa cagggtttga ggaagcggcg gacgatcttc aagacggtaa atcagttcgc
     7681 tgttttgagt ctgcgcaacc ggttgcgcat tttctgactc gagggtggaa acagacatcg
     7741 aaaacaatcc cgtggtagca aagcgggcat tttagctgac cgaaagagaa aagcaaacgt
     7801 ttgccacatc ctcatctaaa tatttaaatg agataaatgc acttttttat ataacttttg
     7861 gtatttttct gcttaaaatc catgccattt gttcattaac agctgagaat ttgacccagg
     7921 aaagcataac tcgatacccg tcatagttca tgaagttgcc actgcgcgat gaagtatgac
     7981 gagtatgaaa gagtgatgcg gatacaaagg agtaactatg tttcatctcg atactttagc
     8041 aacgcttgtt gccgcaacgc tgacgttgct gctcgggcgt aagttggtcc attccgtctc
     8101 ctttttgaag aaatacacca taccggaacc tgttgcgggt ggtttgttgg tggcgctggc
     8161 gctactagta ctgaaaaaaa gcatgggctg ggaagtcaac tttgatatgt ccctgcgcga
     8221 tccgttaatg ctggctttct tcgccaccat tggcctgaac gccaacattg ccagtttgcg
     8281 tgccggtggg cgtgtggttg gcatcttctt gattgtggtt gttggtctgt tggtgatgca
     8341 aaatgccatt ggcattggta tggctagcct gttagggctt gatccgctga tggggctgtt
     8401 ggccggttct attacgcttt ccggcggtca cggtacgggc gctgcgtgga gtaaattgtt
     8461 cattgaacgt tatggcttca ccaatgcgac ggaagtggcg atggcctgtg caacgttcgg
     8521 tctggtgctg ggcggcttga ttggcggtcc ggtggcgcgc tatctggtga aacactccac
     8581 cacgccgaac ggtattccgg atgaccagga agtcccgacg gcgtttgaaa agccggatgt
     8641 gggccgcatg atcacctcgc tggtgctgat tgaaactatc gcgctgattg ctatctgcct
     8701 gacggtgggg aaaattgttg cgcaactttt ggctggcact gcttttgaac tgccgacctt
     8761 cgtctgtgta ctgtttgttg gcgtgattct gagcaacggt ctgtcaatga tgggctttta
     8821 ccgcgtcttt gagcgtgcgg tatccgtgct gggtaacgta agcctgtcgt tgttcctggc
     8881 gatggcgctg atggggctga aactgtggga gctggcttcg ctggcgctgc cgatgctggc
     8941 gattctggtg gtacagacca tcttcatggc gttgtatgcc atcttcgtta cctggcgcat
     9001 gatgggcaaa aactacgatg cggcagtgct ggctgcgggt cactgtggtt ttggcctcgg
     9061 tgcaacgcca acggcaatcg ccaacatgca ggcgatcact gaacgctttg gcccgtcgca
     9121 catggcgttt ttggtggtgc cgatggtcgg tgcgttcttt atcgatatcg tcaatgcgct
     9181 ggtgattaag ctgtatttaa tgttgccgat ttttgccggt taaaaatgaa aaagcgtcgg
     9241 aaggaaccgg aaacgctacg tgaacactgt cgccatattt ttggtgatga gccgccagtg
     9301 ctctgtgtat gggaaacgga atttgattac gcagatgccg aactcaaggc gctggcggct
     9361 aaagagtggc agcagatcag cgaatgggat ttgagtgctt actatgtact caatctcgtt
     9421 tataacgaac cgatgcaaat agagttgttt cgctatctgt ttccgctctg cctggcgcaa
     9481 tggcatgaaa ccgttttagc tggcggttat ggtgatcact tcgaagaatc cttaatgaag
     9541 gcattgtgtc ggccctatct ctggcaggag atgatgaatg cttcccaacg ccagcaggta
     9601 cggcaatttt tactggacac cgcgttgcag cgtatggata acgagcgtgg tttcaataac
     9661 gttctttgct ggctggcggt gttcaatact ctgggtgggg ccgcgccgtt aattcgttct
     9721 ctctggtcgc gctggtgggc gctggatacg ccaggaaaag cggtgtgtgc tattcagtat
     9781 gccgcgcatc taatttatcc catagaagcc aatccgttat ggtcgcaaga atggataggc
     9841 tgggggcatc cattaggcca taaagatggc tggtcctctg ataatcgcgc ttttcttcgc
     9901 caaatgttaa cacctgaaat gattgtggct ggcgtacagg cagcggctga aattctgcgt
     9961 ggtgaaccgg aaggcgcaat ggcggcgcga attgcacagg atgcgtatga ggcaatggat
    10021 atcctcacta tccagattga agatttactg cgtgacttgt cttgtgatga gtctggtcat
    10081 gcgttggagt aacgttccgt ctccggcatc cagcgttcta tcagggcttt tgcttgttgt
    10141 gggtaacgtt cgtgaatatg tcgcgccagg cgctgaactt ccgggatcat cgcctgatcg
    10201 cgcagtaaat ccgccacttt aaactcagca ttacccgtct gacgcgtacc taacagttca
    10261 cccggtccgc gaatctctaa gtctttctgt gcaatcacaa aaccgtcgtt gctgtcgcgc
    10321 agcacttgca gccgaatttg cgcggttttg gagagcggcg ttttgtagag cagcacgcag
    10381 tgagaagcca ccgcaccacg acctacacgc ccgcgaagct ggtgtaactg cgccagaccc
    10441 agacgctccg ggttttcgat aatcatcaga ctggcgttag gcacatcaac gccgacttca
    10501 ataacggttg tggcaaccag caggtgtagc tcaccttgtt taaacgacgc catcaccgcc
    10561 tgtttctcga caggtttcat ccgcccgtgt accaggccaa cgttcaactc tggtagcgcc
    10621 agtttcaact cttcccaggt agcttccgcc gcctgcgctt ccagcaattc cgactcttca
    10681 atcaacgtac aaacccagta cgcctgacga ccttcagtta tgcaggcgtg gcgcacgcgg
    10741 tcaatgatgt cggtacggcg ggtatcagga atagcgaccg tagtcactgg cgtgcggcct
    10801 ggcggcagct catctatcac cgaggtatcg agatcggcat acgccgtcat tgccagcgtg
    10861 cgggggatcg gcgtggcggt catgatcaac tgatgcggat ggaagccttg ctgctggcct
    10921 ttctcccata acgccagacg ctgatgcacg ccaaagcgat gttgttcgtc gataatcacc
    10981 agcgccagac cgttaaactg cacctgttcc tggaagatgg cgtgcgtacc aacaatcatc
    11041 tgcacctgac cgctggcgat ggcttcctgc tgagacagtc gcgctttgcc tttctgctta
    11101 ccggcgagcc agcccacttc gataccgagc ggttcaaacc agttgcggaa gttattggcg
    11161 tgctgttcgg cgagtaattc ggttggtgcc atcaatgcca cctgtttgcc gtgggcaatc
    11221 gcacgcagcg cggcgagggc ggcgaccagc gttttaccgg aacctacatc gccctgtacc
    11281 aggcgcatca tcggcacatc cagcgccata tcgcgctcga tctccgccac tacgcgtgcc
    11341 tgtgcgcccg ttggcttgaa cggtaaggct gcgaggagtt tatttttcag cgcatcattg
    11401 gcgctcagcg gctgggcatg aaaacgctgt gctccggcac gtaaggctaa catgctgagg
    11461 ttgtgcgcca gcagttcttc cagaatcaga cgacgttgcg ccggatgctg cccggtttcc
    11521 agatcgctaa gctgtagcgt cggtggcggg cggtgcaaag tgcgcaacgc ttccggtagc
    11581 gtcatcattc cttgtgacag ttccggcggc aggagttctt caatggcgca ggtgtcgagc
    11641 agatccagcg cctggtcggt taatttacgc agcgtggcct gctttacgcc ttccgttgtt
    11701 ggataaaccg gcgtgagcgt ttcctgtaat tctggcgtgc tgagatcgcc ctgcacgcgg
    11761 tattccgggt ggatcatctc cgcaccatat ttaccgcgct ttgcttcgcc ataagccagt
    11821 acacggcggc ccgtcgccag gctatttttc attgccgcgc tgaaattgaa aaagcgcatg
    11881 gtgaggatgc cggaaccgtc gctgatctgg caggtcatca tccgccgacc gccgaaggag
    11941 atattgcagt tcagcacttc gccttccacc gtggcataaa cgcccggcag tagttctccg
    12001 atggggtaga gatgggtgcg atcttcgtag cgcaggggaa ggtgtaagag tagatcctgc
    12061 acggtatgca ggttgatttt cgccagtttg ttactaagtg ctgcgccaac gcccgttagg
    12121 gaactgagtg ggacagcatc taacaggcga cctttcatgg cacttaccct gcagcctgca
    12181 tagtagccca ccagtcggca tcagcttcga tctcgccttg ctgattgacg tggggataag
    12241 gcaggccttt gcgttttgcg actttcgcca gcaccggata gccgccttca aacaacaggc
    12301 gttgttgctc tgcttccggc aacatgctgt tttcacgcag gtacatgcct gcattttgcc
    12361 gctgacgctg ggcttcgtaa agaatgaggg ctgaggcaac ggaaacattc agcgactgca
    12421 ccatgccgat catcggaatg atgatgtcct gatccgccag ggccaatgct tcctgcgtga
    12481 tgcccgtttt ctcctgtccc atcaaaatgc aggtcgggcg agtgtaatca atttcgcgga
    12541 aatcgacagc gttatcagaa agatgggttg ccagaatctg catgccccgg cctttgagat
    12601 gagcgacggc atcgccaatg gtgcggtgtg ttttcacctg tacccagctg ttactacccg
    12661 ccgctgccga agccatggtg cgcatgcggc taccaggcca gacggcgtga acttcatgta
    12721 cgccaacggc atctgcggta cgaataatcg cagaaacgtt atgaggtttg tggacctgct
    12781 ccatgcagac ggtcagatca ggctgccgcc tggcgagcat ttcgcagatg cgtgcataac
    12841 gtgttgggtt cataaaacat taatttcggt ttcgggtgac tttaatcacg tctggcatca
    12901 cgcggatttt gcgcatgata ttcgccagat gcacacggtc acgggcggtc agacgaataa
    12961 aggcgctgta gacgcgacca tctttctctt ccgtattcaa actttgaata ttcgaagtcg
    13021 tggtgttaat tgccgccgtc aggtttgcca gcgcgccctg atgattgaac atctccacct
    13081 tgatttcggt gatgaactcc tgcgccgtct ctttatccca ttccacagcc ataaacttct
    13141 ctggctcttt ctggtagcca cggatattac ggcaggattc atggtggatc accagacctt
    13201 taccggggct gacgtgggcg ataatcgggt cgccaggaat agggcggcag catttcgcaa
    13261 aggtgatcag cacgccatcg gcacctttaa tgggcagatg tccgtggctt tgggttgccg
    13321 gtggaatgga ggcgtcccca tgttgcagat ttttcgcgac caccacgctc attgcgttac
    13381 caagtccgat ttctgccagc agatcgtcaa gcgttgccag cttcatgcga tccagctcgc
    13441 gctgaatatt ttcctgcggg atttcattca gcttacggct accacccaaa gcatggttga
    13501 gcagacgacg gcccaggctt acagaatcat cacgcttgag gtttttcagc aactgacgaa
    13561 ttttggcgcg cgcttttgag ctaacgacaa agttcagcca cgcggcattc gggcgagcgc
    13621 ccggtgcggt aatgatttca acggtttgac cgctggtaag cggctgcgac agcgggtaag
    13681 gctggcggtc aacgcgtgcg cccacgcagg catgaccgat atcggtatgc actgcataag
    13741 cgaagtcgac gggcgttgca ccggcaggca gctcgacaat tcgcccttcc ggtgtgaaaa
    13801 cgtaaatctc atccgggaag agatcggatt taacgctctc gataaattca aacgaactac
    13861 cggcgctctg ttgcagctcc agcaggcttt gcatccagcg ctgggcgcgg atttgcgcgg
    13921 tggtgctggt ttcgccgtgc tctttataag cccagtgcgc ggcaacaccc atctccgcca
    13981 tctgatccat atcttcggta cggatctgga cctcaaccgg cacaccgtgc gggccgatca
    14041 tcgaggtgtg caaagactga tagccgttcg cttttggaat ggcgatgtag tctttcacgc
    14101 gacccggacg cggcttgtac aggctatgca tttgcccgag cacgcgataa caggtgtcag
    14161 aatcattgac gatcacgcgg aaagcgtaga tgtccatgat tgagtgaaaa cgctgctctt
    14221 tgagcaccat tttgcagtaa atcgaataaa gatgcttttc gcgaccactg acgcggcacg
    14281 gtattcccgc ttcctgcaaa cgcccttcga tttcagagag gattttttgg atcatctctt
    14341 tacggttgcc gcgcgcggct ttcaccactt ctttaattac gcggtaacga ttgggataca
    14401 gcgcctcaaa acccagctct tcgagttcgg ttttaatgtg gtggatacct aaacggtgcg
    14461 ccagcgggct ataaatttcg agagtttcac gggcgatgcg gcgacgtttg tccgggcgaa
    14521 gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag tttgatgagg atgacgcgga
    14581 tatcctgcac catcgccata atcatcttgc gaaagttttc ggcctgcgcc tctttcttat
    14641 cgcggaactt gagtttatca agtttcgaca ccccctctac cagctcggcg acgcttttac
    14701 caaaaagctg ttccatatcc tggtaggtgg cgggagtatc ttcaatcacg tcatgcagca
    14761 gcgccgccat cagcgtttca tagtcgagtt tcatctcggc cagaatgcag gcaaccgcta
    14821 ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg cccctcgtga gcatcacgtg
    14881 caacgagata cgcctgccgc agacgcttga tttggtcttc cggcaggtag gtttgaatca
    14941 gttgattcag gctttcaaac agatacaagg gcgacccgct ttgtgattaa cgacgacctt
    15001 cagcaatagc ggtaacggct tgtaattcag cggcttcctg ctcttgctgt tcctggcgtt
    15061 cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc gatttcgcgc agcgcgatta
    15121 cagtggtttt atcgttttct tccggtacca gcggatcctt tccgcctacc tgcatctgac
    15181 gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt accaattttc tctacagcgt
    15241 cctgaacagt tacgcgtgcc atacttaaaa agctccacag gtgaagaaat gactgggcat
    15301 gatactgaaa tcaggttcag tctgccaaca atttgctgat taaagcgtca tgacgctgct
    15361 tttggcggct catgcgcaga cgttcggcgc gaataatggt cttcaaatcg gtcaacgcgg
    15421 tatcgaagtc atcattcaca atcagataat catattcggc gtaatggctc atttctgcaa
    15481 cagcttgcgc catacgcttt gcaatgacct cttcgctgtc ctgaccgcga ccgcgtagac
    15541 ggcggtccag ttcaattttg gacggcggta aaataaagat actccgcgcg tgcggcattt
    15601 tctggcgaat ttgctgcgcg ccctgccagt cgatatcgag aaaaacatcg acaccggtcg
    15661 ccagtacttg ctcaatggcc tcacgcgaag tgccatagta attaccaaaa acttctgcgt
    15721 gttcgaggaa cgcatctctg ctaatcattt ctttaaattc atcatgatta acaaagaaat
    15781 aatgttcacc gtggacttca ccaggacgcg gttggcgtgt ggtgtgtgaa acagaaacct
    15841 gggtgtcata caacggttgg gtttttaata aagcctgaat caggctggat ttacccgcgc
    15901 cactgggggc agaaacaata taaagcgtgc cttgagccat gagtatcttt cgtatgtgat
    15961 tagcgaaata aagcctacat acgagcttat tatacacggc gcggccatgt gacgtagccc
    16021 ttgctcacac tttttgcgca aagttgttgc gttttgcgta gctgtttccg catttgcctg
    16081 tgatttactg caataaagac gattctcagg aagcgtcctc gcagactccg tttttgccac
    16141 tcggctttgc cttcaccacg ttgtatatct gccagcctga ctacagcgga ggtgaagatg
    16201 aaagtatgga tggcgatatt aataagtatc ttgtgctggc aatcatctgt gtgggcggtc
    16261 tgtccggcct ggtcgccagc cagagcacag gaagaaattt cccgcctgca acagcaaata
    16321 aaacagtggg acgatgacta ctggaaggaa ggaaaaagtg aggtggaaga cggtgtttac
    16381 gatcagttaa gcgcccgtct tacgcagtgg caacgctgct ttgggagcga gccccgcgac
    16441 gtgatgatgc cgcctttaaa tggtgcggtt atgcatccgg ttgcccatac gggtgtacgc
    16501 aaaatggtgg ataaaaacgc attaagtctg tggatgcgag aacgtagcga tctttgggtg
    16561 cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa actgaacaaa
    16621 gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga cgcagaaagt tagcttaatt
    16681 tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gtacgcttca gggggaaata
    16741 tttctccagc gcgaggggca tatccaacaa caaatggggg gaataaatgc ccgcgcaaaa
    16801 gttgctggcc tgatgatgcg ccaggacgat agcgacacgc tgaattctct gggcgttttt
    16861 gtctgggcat ggccggatgg accgcagtta atgaccgatc gtttaaagga gctggctacc
    16921 gcaggtttta ctctaacgca gaggtatacc cgtgcggtta aaaatgctga tgaggttgcg
    16981 cgcgtacgca atgagtggtg gaaagcgaaa ttacccttcg tcaccgatgg cgtggttgtg
    17041 cgaggggcga aagaacccga atcccgccat tggctaccag gccaggcaga atggctggtg
    17101 gcctggaaat atcaacctgt agctcaggtt gccgaagtga aggcgattca gtttgcggtg
    17161 ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac ctgtcatgct ggatgataaa
    17221 aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga tattgcgcct
    17281 ggtgatcaga tcctcgtcag ccttgccggt cagggtattc ctcgcattga tgatgtggtc
    17341 tggcgcggtg cagaacgtac aaaaccgaca ccaccagaaa accgctttaa ctcgttgacc
    17401 tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt ctggctgggg
    17461 tcaaaacagg ttctggggct ggatggcatt ggtgaggccg gctggcgcgc gctgcatcag
    17521 actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca attacagaac
    17581 acgccgggga tcgcgaaaag taaaagtgcg cagctatggc atcagtttaa tctggctcgt
    17641 aagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg ggcggcgctt
    17701 aatgccagtg atgaacggtc ctggtcgcaa cttttattta gcacggagca gttctggcag
    17761 cagctgccgg ggactggatc ggggcgcgcc agacaggtta ttgaatggaa ggaaaatgcg
    17821 caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt tgaaccttag
    17881 tgtccttcgt ggctgtagta aaagaccggt aatcccagct taaggcgtag cgccagtaag
    17941 cgggcaaaaa agccgaatac cagggtggag ataattacca catcatggtt agaaacatag
    18001 tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata caactctttc
    18061 tggaatacca gtgggatacg tttacagaac atatcgcgca aaacgccgcc aaacacgccg
    18121 gtggttaccg ccgcgacaac ggcaataatt gggccgtgcc ccatatccag tgcaacctgt
    18181 gccccgatga tagaaaagac caccagtccg agcgcatcga gcaccagaaa cactttgcgc
    18241 aggtaaggca ttacaggagc aacgatagtg gtgagtactg cggcggtagc gacgataatc
    18301 acatattccg ggtgtttgac ccagccgagc ggatagtggc ccagcagaat atcgcgcact
    18361 gaccctccgc caattgcggt ggcggtcgca ataataatta cgccaaatgt atccatgcgc
    18421 cgtcgtccgg ccgccagcgc ccccgtcatg gcttcggcag taatgccaac caaatacaaa
    18481 atgtgtaaca gcatggttct ctccctaaaa atctgtctgg cagggtagcg atttgtgcgc
    18541 attgtcacga ttgagatttt ttaaggtgag gggaaattga taacttgtag ttatgtgaaa
    18601 tcgctatttt ctgtagcgta gatgcattct tctgacttct tgtctgagaa aatgcataag
    18661 aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc aatgtttagc cattgtgttc
    18721 accgcacctg gactttggct tacgcatcgg gtatctcaat caacaaaaag agtttttagt
    18781 ttctgctatt tcttctctgt aatttttaca ctgttttcca actgcttgat gctgacctgc
    18841 ggggtcggca actcctcagg catggttccg ccaagttcct gaatggtttg cctcaccttg
    18901 ctacccacgt caaagtgtgt ggtattagct tgctgttttg aattcacgcc atcccgcttg
    18961 agtttttctt ctgtttgggt agctcgaaag agattagccg ccagttctgt tgaacccata
    19021 tgatccagga tcttctgact ctttttcagc cccttccgct gatggatggc tttctgatct
    19081 aatccaccat acagcccccg gtaaccatga ttctgaaata tggcgaaatc gatagctgtc
    19141 gctaccccag cttgctgcgc tgcctcaacc aattgtttgt tatgttcttt caattcattt
    19201 ctcagaaaaa gacgcttttc gtcttcacga agttgcctga aagcttcatc gtcagccagc
    19261 tcctgccgtc gggtctggat agcaaaataa gtttgccctg ccgcaataac cggtttcgca
    19321 gggtcgccgt tttgtactac caggtaacag gcatagcggg agagatgaac atcttcaagt
    19381 tctcgttgag cccctgaacc taatacaacc attttggtgg tttcaacgaa atggtcagat
    19441 gcagcttgat tgctggcttc gcacgcttga gtcgcgcggg ccagtacctt ttgaaaattg
    19501 cgccagtccc gataatccag taacggtgcc aattctctag ctgaccaaaa ttcttgccct
    19561 tcagtgccat aatgccgtat ttcttcaaaa ggttgatgat gttcgttcat tgcccactcc
    19621 atgtgatatc gattgcaaag aaagtaaatc tgtaactgta tttatataca gttgtgagta
    19681 tctcacgttc tcagtgtgct gggcaaaaaa acatcgcaaa aaaacatcgc aaaatctgga
    19741 gcaggcacgc aaaacagaat tgaagttgcg gggaagaaaa atgccgctct gtttgagtgg
    19801 gcggcatttg tgaaggctga aaagtaagaa ttggcgttac gagttattca atgttctgaa
    19861 tctgctcgcg catctgctca atcaacactt tcagctcaat cgcggagttt gtcacttcgg
    19921 cattgataga cttcgacgca agagtgttcg actcgcggtt gaattcctgc atcataaagt
    19981 ccagacgacg accaacggct tcttttttct tcagaatgtt gtaggtctct ttgacatgcg
    20041 cttccaggcg gtccagttct tcggcaacgt caattcgttg tgccagcaga accagctcct
    20101 gctccagacg gttgttttcc aactgaacct gagcatcttc cagcttcgcg accagacgct
    20161 cacgctgcca ttgcaggatt tccggcatat gggcgcggac tttgaccact tcggcggtga
    20221 cgccttccag acgctgctcg atcaatgctt tcagtgcctg accttcggtt tcgcgcgcga
    20281 caataaagtc gtccagcgta ccatccagcg ccgcgagaat ttcagcggca atggcgtcaa
    20341 gatcctgctc ctgggctgcc atcacgcccg gccagcgtag aatatcaacc gggttgattt
    20401 ccccttcgtc actctgcatt tttacccagt tcgcggcagt taccagctgt ttagccagtt
    20461 tttcgttgag gatcagctca ccttgcgcgc taacatctgg ctcatagcgc agggtacatt
    20521 ccactttacc gcgcgtcagg cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac
    20581 ggaactgctc cggcagacga aagtaagttt ccagataacg ctggtttacc gagcgcattt
    20641 cccaggttgc gctcccccat tcacccttga tttcacgccg ggcgtaggcg gtcatactgc
    20701 ggatcataga cgttcctgtt tataaaagga gaggtggaag gattatagcc atcgatgcct
    20761 tgtcaggata ggaataaccg ccggaagtcc gtataatgcg cagccacatt tgtttcaagc
    20821 cggagatttc aatatgcgtc cagcaggccg tagcaataat caggtgcgtc ccgttaccct
    20881 gactcgtaac tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa
    20941 agtgttgtgt accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca
    21001 gggctggatc accgcagagt acggcatgct gccacgttct acccacaccc gtaacgctcg
    21061 tgaagcggcg aaaggtaagc agggtggacg cacaatggaa atccagcgtc tgatcgcccg
    21121 tgctcttcgc gcggcagtag atttgaaagc gctgggtgag ttcaccatta cgctggactg
    21181 cgacgtgctt caggctgatg gtggcacgcg taccgcgtcg attacgggtg cctgcgtggc
    21241 gctggcagat gcgctacaga agctggtgga aaacggcaag ctgaaaacca atccgatgaa
    21301 agggatggta gccgcagttt ctgtcggaat tgtgaacggc gaagcggttt gcgatctgga
    21361 atacgttgaa gactctgccg cagagaccga catgaacgta gtgatgaccg aagacgggcg
    21421 catcattgaa gtgcagggga cggcagaagg cgagccgttc acccatgaag agctactcac
    21481 cttgttggct ctggcccgag ggggaatcga atccattgta gcgacgcaga aggcggcgct
    21541 ggcaaactga tttttaaggc gactgatgag tcgccttttt tttgtctgta gaaaagtaag
    21601 atgaggagcg aaggcatgaa accatatcag cgccagttta ttgaatttgc gcttggcaag
    21661 caggtgttaa agtttggcga gtttacgttg aagtccgggc gcaaaagccc ctatttcttc
    21721 aacgccgggc tgtttaatac cgggcgcgat ctggcactgt taggccgttt ttacgctgaa
    21781 gcgttggtgg attccggcct tgagttcgat ctgctgtttg gccctgctta caaagggatc
    21841 ccgattgcca ccactaccgc cgtggcgctg gcggagcatc atgaccttga cctgccgtac
    21901 tgctttaacc gcaaagaggc aaaagaccac ggtgaaggcg gcaatctggt tggtagcgcg
    21961 ttacaaggac gcgtaatgct ggtagatgat gtgatcaccg ccggaacggc gattcgcgaa
    22021 tcgatggaga ttattcaggc taatggcgcg acgcttgctg gcgtgttgat ttcgcttgat
    22081 cgtcaggaac gggggcgcgg cgagatttcg gcgattcagg aagttgagcg tgattacaac
    22141 tgcaaagtga tctctatcat caccctgaaa gacctgattg cttacctgga agagaagccg
    22201 gaaatggcgg aacatctggc ggcggttaag gcctatcgcg aagagtttgg cgtttaaaga
    22261 aactcgccgg atgaaaagtc atccggcgtc atattactgc aactgtgccg caattagcgg
    22321 ccagcgggcg tcaaaatcat ccgtcgggcg gtatttaaat tcgctgcgga caaaacgtga
    22381 cagcatacct tcacagaagg ccaggatctg gcttgccagc agggtttcat cggtggtgta
    22441 accttcaccc tcacgcattc tcttttcacg caatacctga cgcagctgcg cttcaatacg
    22501 ctcgaacagc tggttgatgc gcccttgcag gcgatcctgt tcaaacatta gcgcatgacc
    22561 agtgaggatg cgggtcaggc caggattacg ctcaccaaaa ccgagaagca gcaacacaat
    22621 cagacgcagg cgcgctgtgg tgtctttctc atctttcaga atcagattga tgcgagtaat
    22681 caggctatct tcgataaact caatcaggct atcgaacatg cgggtcttac tggggaagtg
    22741 gcgatacagt gccgcttcgg aaacgccgac agaggcggcc agttttgccg tcgtgatacg
    22801 ttggcttcca tcgctggatt ccagcatcag cgccagagac tgaagtattt cctcgcgacg
    22861 gttccttttc gcagtttgtt tttctgccat gttacaaaat acccctgaaa ataagcactt
    22921 gccaggcggc acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta
    22981 ttcggatgcg tatgtgttac tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg
    23041 tggcgtcgaa atcttccacc agattaaatt cagcctgtac taccggaaca aaaatcatct
    23101 gggcgatgcg ttcgccaggt tgaatggtga agctgtcctg accacggttc cacacggaaa
    23161 tcatcaactg gccctgatag tcggaatcga tcaatcctac caggttacca agcacgatac
    23221 cgtgcttatg tcccaatccg gagcgcggca gcatcattgc cgccagtgaa ggatcggcaa
    23281 tatgaatcgc cagcccggtc ggaaccagcg tagtgtcacc cggagccagt tctacggcgt
    23341 cgtcgagaca ggcacgcagg tcaagtccgg cagagccaga ggtggcataa gtcgggagcg
    23401 gaaattcctt cccaacgcgc gggtccagaa tcttaacgtc gatttttttc atcataacgg
    23461 gtcacgatct cgtcgagtaa taattggcca aggagctctt tgcgctcaag cggtaagact
    23521 ttatctccgt cctgccagaa aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt
    23581 ggctgggaaa catcgttcgc gcagatcaga tcaaggtttt tacggatacg tttttgccgg
    23641 gcgtattctt ccacattatt tgtttcggcg gcaaatccaa cgacgtaggg tcgatggtct
    23701 tttagtgcgg caacgcctgc gacgatatcg gggtttttaa ccatttttat tgttaattca
    23761 tcaccctgcg tggcctgctt tttgattttc tctggggcca cggtagctgc gcgataatcc
    23821 gccacggcgg cgcagccgat aaaaatattt tgctgctgta cagaagcatt cacggcggct
    23881 tccatttcca gcgcggtcat cacatcaaca cgtttaacaa acggtggcgt cggtagtgaa
    23941 accggacctg ataccagcgt gacgttcgcg ccacgacggg cagcggcggc ggcgatagca
    24001 aaacccatct tgccggagct gtgattagag atataacgca cgggatcgag cggttcacgc
    24061 gtcgggccgg cggtaatcat aatgttcaga tgtttcaggt cgttgacggg cgaaagatgc
    24121 gctaccgcca tatccacaat ggttaacgga tcgagcattc gcccaggacc gatatcacca
    24181 caagcctgac tgccactgtc tggcccccag atgagcaaac cacgggaagc aagcacctct
    24241 aaattatgct gcgtggcagc ggcacggtac atctgctggt tcatggcggg gagcacggct
    24301 acaggcgcag gtgtagccag acaaatcgtc gataccaggt cattcgccat tccggcagca
    24361 acacgggcaa tcaaatctgc cgtggcaggg gcgagaatca ctaaatcagc ccatttaccc
    24421 agctcaatat ggcccatagc ggcttcggct gccgggtcca gcagactgtc ggaaacggga
    24481 taaccagaaa ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc ttcggtcatg
    24541 gctacgcgga cgtcggcccc gcgatcgcgc aaacgacgca ccagttcagg ggttttatag
    24601 gcagcaatac cgccgctaac gccgagaacg atttttttac cggccaggct catgatgatt
    24661 tttcctgttg ggtgacacca gaagttggcg attttatcac aatacttttg ttgtcgtgcc
    24721 ttcacctgag aatcactttg cgaggcgctt tccaggattg aaaactggcc gtcgatttaa
    24781 cggaacggct atgacaggat gcgagcacca caaaggaggt gaaggtgaaa aacaattcac
    24841 agctgttgat gccgcgcgaa aaaatgctga agtttggtat tagcgcctta acggatgtcg
    24901 agctgctggc gctatttctg cgtaccggaa cgcgcggtaa agatgtatta accctggcaa
    24961 aagagatgct ggagaatttc ggctctcttt atggcttgtt aacctctgaa tatgaacaat
    25021 ttagtggcgt tcatggaatt ggcgtggcga aatttgccca gttaaagggg attgctgaac
    25081 tggcgcggcg ttactacaac gtgcggatgc gtgaagaaag ccctttactc agcccggaga
    25141 tgacgaggga atttttacaa agccagctca cgggtgagga gcgggagatc tttatggtga
    25201 tctttctcga ctcccaacac cgggttataa cgcatagccg tcttttttcc ggcacgctaa
    25261 accatgttga agtccatcct cgggaaatta tccgcgaagc gataaaaata aacgcctcgg
    25321 cgctgatcct tgcacataat cacccttcgg gttgtgctga acccagtaaa gcggataaac
    25381 tcattactga acggataata aagagttgtc agttcatgga tttacgcgtg ctcgaccata
    25441 tcgtgattgg gcgtggagag tatgtttctt ttgccgaacg cggctggatt taacccgcta
    25501 tgcgcgatcc ttcgggatct ttgtctgttc gggacttgag cacatcgctg agtcagcgta
    25561 tactacgcca cctttgagaa tctcgggttt ggcatttggg cctggcaatc gagagttcac
    25621 atagaactgc gatgaccggg ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct
    25681 cgagctaatt tgatttttgg agaatagaca tgtcccgagt ctgccaagtt actggcaagc
    25741 gtccggtgac cggtaacaac cgttcccacg cactgaacgc gactaaacgc cgtttcctgc
    25801 cgaacctgca ctctcaccgt ttctgggttg agagcgagaa gcgttttgtc accctgcgcg
    25861 tatctgctaa aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt ctggctgaac
    25921 tgcgtgcccg tggcgaaaag tactaagtac ttagaggaaa taaatcatgg ctaaaggtat
    25981 tcgtgagaaa atcaagctgg tttcttctgc tggtactggt cacttctata ccactacgaa
    26041 gaacaaacgt actaagccgg aaaaactgga actgaaaaaa ttcgatccag ttgttcgtca
    26101 gcacgtgatc tacaaagaag cgaaaatcaa ataattctcg ctttgatgta acaaaaaacc
    26161 tcgctccggc ggggtttttt gttatctgct tgcccccata ttgattgcat ctgttcattc
    26221 ctggagatgc tatgcctgaa ttacccgaag ttgaaaccag ccgccgcggc atagaaccgc
    26281 atctcgttgg tgcaaccatt cttcatgcag tggtgcgcaa cggacgcttg cgctggccgg
    26341 tttcagaaga gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag cggcgggcta
    26401 aatatctgct gctggagctg cctgagggct ggattatcat tcatttaggg atgtctggca
    26461 gcctgcgcat ccttccagaa gaacttcccc ctgaaaagca tgaccatgtg gatttggtga
    26521 tgagcaacgg caaagtgctg cgctacaccg atccgcgccg ctttggtgcc tggctgtgga
    26581 ccaaagagct agaagggcac aacgtgctgg ctcatcttgg accggagccg cttagcgacg
    26641 atttcaatgg tgagtatctg catcagaagt gcgcgaagaa aaaaacggcg attaaaccgt
    26701 ggctgatgga taacaagctg gtggtagggg tagggaatat ctatgccagc gaatcactgt
    26761 ttgcggcggg gatccatccg gatcggctgg cgtcatcact gtcgctggca gagtgtgaat
    26821 tgttagctcg ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggt ggtacaacgc
    26881 tgaaagattt tctgcaaagt gatggtaaac cgggctattt cgctcaggaa ttgcaggttt
    26941 acgggcgaaa aggcgagccg tgtcgggtgt gcggtacgcc gattgtggtg actaaacatg
    27001 cgcagcgggc aacgttttat tgtcggcagt gccagaagta attcatgcgc gccggatggc
    27061 ataccatccg gcataaacgc tacgctaact tcgccatcag cgcctgatgg acattctccg
    27121 gcaggaaatg ggtgacatcg ccctgatggc gcgccacctc tttcaccaac gatgaagaga
    27181 taaacgacca ctctttcgac ggcatcagaa acacactttc cagttccggc attaagtggc
    27241 gattcatatg cgccagctgc atttcatatt caaaatccgc caccgcacgc aggccacgaa
    27301 tcagcaccgt agcgtgttga ttacgggcga agttcgccat caaatcacta aacccgacca
    27361 cttccacgtt ccccagatgc gcggttgcct gctgtgccag cgccacacgc tcttccaggg
    27421 taaacatcgg ttttttactg gggctggcgg caatcgccag aataacgtga tcgaacattt
    27481 gcgtggcgcg cgtcacgata tcgatatgac cattggtaat gggatcgaaa gtacccggat
    27541 aaatcgcccg tttttgcata acaacctcaa tgcgttttcg gtggcaggta aggtttcagc
    27601 agttgaagca gacgctgtag cgcgccctgg ttttgataca gtacttcaac ggcatgacgg
    27661 ccatagaaac tacggtaatc ggcgtcggtg agtaaagagg acacctcttt tgcaagcgta
    27721 gtggcatcgg taacggtaat cagcccgctt gcctgctcca gccgcgcgca aatgtcttta
    27781 aagttaaaag tatgcggccc catcaatacc ggaatagcgt gtgcggcagc ttccagcgga
    27841 ttatgcccac cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac
    27901 agtaacatca actcgcccat cgtatcgcca actacaacct gcgtgctggt ggaggggact
    27961 tcccctgaag agcgtgtgat atagcttagt ccagcctggc ggacaagatt aatcgcatcc
    28021 gggaagcgtt ctggatgacg gggtaccagg atgagcaata aattcgggaa ttgctgtaac
    28081 aatgcctgat gtgcggcgat caccacactc tcttcgcctt cgtgagtgct ggtggcaatc
    28141 cataccgggc ggtgtggtgc ccactggcgg cgcagcgtca cggctttagc agccaactgc
    28201 ggcgttacag aaatatcgaa tttcaggcta ccggtaacgg tcacctgatt attttttgcg
    28261 cccagcgcca caaaacgtgc accatcttct tcattttgcg cagcaatcag cgtaatacga
    28321 cgcagcaagc ggcggacgaa tttacccagt ttggcataac ctgcggccga gcgggcagag
    28381 agtcgcgcgt tagcgatcac cagcggaatt ttacgtttat gtagcgccgc aatcaggtta
    28441 ggccatagtt cggtttccat aatcaacacc agtttggggt cgactttatt caggaaacgg
    28501 ttgagcgcat cgggcaggtc atacggcaga taaacgtgct gaacatcctt cccgaaagcc
    28561 gattgtacgc gctccgaacc ggttggcgtc atggttgtaa cggtaatcgg taaatcagga
    28621 taacgatgac gcagcgcgcg caccaacggg atcgccgcca gagtttcacc gacggagacg
    28681 gagtgcagca taatgccgcc tggttttagc ggatggcggt aaaaaccgta acgttcaccc
    28741 cagcgttttc gataggccgg agccttacgt ccgcgcaccc agagccgtat ccagatcagc
    28801 ggctgaataa ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa tagctgactt
    28861 atggatgtgc tggggattct atgtatttag ctgtggcttt accattactt ttcccgtttt
    28921 tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt
    28981 ttatgcgatt cattgatttc tgctgtacgt gcaggaagga atcgcagagc aacagtgaaa
    29041 gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca acaatgtgct
    29101 taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg tcaatatcag
    29161 tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt tttctgcttc
    29221 accttaatcg gataatctca acaaaaagag ttacttgtgg ataagccatt tcgaagaatt
    29281 ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt cattagttcg
    29341 ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga caccatcccg
    29401 atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa aaaagcaaaa
    29461 gcctcagaaa aaattgccaa cttttttcat ctcatcaagg tattacgtgc caataagtat
    29521 gaccttatcg tcaatctcac cgatcaatgg atggttgcta tactggttcg cttattaaat
    29581 gcccgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg gcgtaaaagt
    29641 ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt atccgtgctg
    29701 accccattgg gagttgattc gttggtgaag cagacaacca tgagttaccc gcctgcaagc
    29761 tggaaacgta tgcgtcgcga acttgatcac gctggtgttg gacaaaatta tgtggttatc
    29821 caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt
    29881 gatgccttac atgctcgtgg ttatgaagtt gttctgacgt ccggcccaga taaagacgat
    29941 ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac ggcgctggct
    30001 ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct gtttattggc
    30061 gttgattccg caccggcgca tattgccgct gcagttaata cgccgctgat atcgctgttt
    30121 ggtgcgacag atcatatttt ctggcgtccc tggtcaaata acatgattca attctgggcg
    30181 ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg
    30241 gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc ctccacgaca
    30301 ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt ggcggtttgc
    30361 agcgtgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat gttcgggttt
    30421 atacccagtc gtgggaaggc gaatgccctg atgtatttga actgatcaaa gtgccggtta
    30481 aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac
    30541 gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg gacgtttatt
    30601 atgccgctga tgtttgttat gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc
    30661 tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa cagggtaagc
    30721 cgacacagtt gctgatgctg acagataagc aaatcgccga tttccagaaa cattatcaga
    30781 ctgaagcgga gcgttttcat attctgccac cggggattta tcctgatcgt aaatatagcc
    30841 agcagccagc caatagccgt gaaatcttcc gtaagaagaa tggaataacc gaacaacaat
    30901 atttattgtt gcaggtcggt tcagacttca cgcgtaaagg tgtcgatcgt tccattgaag
    30961 cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt gttgggcagg
    31021 ataaaccgcg aaaatttgag gcgctggcag aaaaacgcgg cgtgcgcagt aatgttcact
    31081 ttttctcggg gcgcaatgat gtctcggaat taatggcggc ggcggattta ttactgcatc
    31141 ctgcctacca ggaagcggcg ggaattgtgc tgctggaagc gattactgca ggattaccgg
    31201 tactaacaac agccgtttgt ggctatgcgc attatattgt cgacgctaat tgcggcgagg
    31261 ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa gcgttaacgc
    31321 aatcttcatt gcgccaggcc tgggcggaaa atgcgcgaca ttatgctgat acacaagatt
    31381 tatatagtct gccagagaaa gcggcggata tcataacggg tggtctggat ggttgaactt
    31441 aaagagccgt ttgccacgtt atggcgcggc aaagatcctt ttgaggaagt taaaaccttg
    31501 cagggtgagg tatttcgtga actggaaact cgccgtactc tgcgctttga aatggcgggc
    31561 aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat caaaaattta
    31621 ctctcattgc ggatgccagt attaggcgct gaccgcgaat ggaatgcgat tcatcgactg
    31681 cgggatgtcg gcgttgatac tatgtatggg gtggcatttg gcgaaaaagg catgaatccg
    31741 ctgaccagaa cttcatttat tattaccgaa gatctgacac caaccataag tcttgaagat
    31801 tacagtgctg actgggcgac taaccctcca gatgttcgcg taaagcgtat gcttattaag
    31861 cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccaccg tgactgttat
    31921 atctgtcatt tcctgctgca cttgcctttt tccggtaagg aagaggagtt aaaaatttcg
    31981 gtaattgacc tgcaccgggc gcagcttcgc acgcgcgttc cacgtcgttg gcgggataaa
    32041 gatcttattg ggctttattt ttcttcgatg aatatcggcc tgactcagcg ggatatctgg
    32101 cggtttatga aagtgtattt tgccgccccg cttaaagaca ttctcaagca ggaacaagga
    32161 ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat tcgaaaatcg
    32221 ttgtaacccg taacttattt ttgccaaaat tttggataca gaataaatat gtctcaactc
    32281 aatgatagtg acatcatcct ttttgagtat aattttcatt atcaaaatat aagatctaaa
    32341 aatactcttg atatagcatt
//
LOCUS       DAMZII010000044        32125 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZII010000044 DAMZII010000000
VERSION     DAMZII010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.044"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(46..921)
                     /locus_tag="POA77_002014"
     CDS             complement(46..921)
                     /locus_tag="POA77_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365456.1"
                     /translation="MTKRIPVAEIVEALSKWFDVSRYDALKDLTLEQIYAELERRMFV
                     YKARQQWETLDDKHRNAVIHHDAMIHSGRVLLEDKWISESHMLSHSYAVRPMTRNSLF
                     NYGRAMYRLENTPQEENVSVSSDYISEYLKQGGLNPANKMLIEIDLEEASSDDLAEHL
                     KVLISQWQKHLKVPKPPEKDFRFGHKTFQKILDYKIIPLMDLIAWEQLNNQKIKYPVL
                     AGILHPDMRYARGSEQIKDTDYPLAHGFLSNDNYFKSLNDFFIKNNLVKNSPILDVIA
                     MNDKPETKKKTRDIH"
     gene            1462..2724
                     /locus_tag="POA77_002015"
     CDS             1462..2724
                     /locus_tag="POA77_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6365457.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGVRKMLALNINPVQQRAAERGSRTPDKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKRIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQGLIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRLAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMSHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(2918..4222)
                     /gene="ybtS"
                     /locus_tag="POA77_002016"
     CDS             complement(2918..4222)
                     /gene="ybtS"
                     /locus_tag="POA77_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDI6365458.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCLARLHINADGTQATFIDDAGEQQWAVDSITDCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGGRRLCEWVKEASTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCLPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDMRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4250..5530)
                     /gene="ybtX"
                     /locus_tag="POA77_002017"
     CDS             complement(4250..5530)
                     /gene="ybtX"
                     /locus_tag="POA77_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDI6365459.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LMIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRAGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAMLIMALSFPLWRITEPTRTAPI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTFMMAPGHLLLPILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGVFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5523..7325)
                     /gene="ybtQ"
                     /locus_tag="POA77_002018"
     CDS             complement(5523..7325)
                     /gene="ybtQ"
                     /locus_tag="POA77_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDI6365460.1"
                     /translation="MKDNNPADNLAWRVNWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLQLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIERFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(7312..9024)
                     /gene="ybtP"
                     /locus_tag="POA77_002019"
     CDS             complement(7312..9024)
                     /gene="ybtP"
                     /locus_tag="POA77_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDI6365461.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VSQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            9281..10240
                     /gene="ybtA"
                     /locus_tag="POA77_002020"
     CDS             9281..10240
                     /gene="ybtA"
                     /locus_tag="POA77_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDI6365462.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPATLALWVPDISADIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRVM"
     gene            10431..16538
                     /gene="irp2"
                     /locus_tag="POA77_002021"
     CDS             10431..16538
                     /gene="irp2"
                     /locus_tag="POA77_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDI6365463.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCITDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPIRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQQQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWFDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEQSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     TTVEKTKRPRPVRRRQRRI"
     gene            16626..26117
                     /gene="irp1"
                     /locus_tag="POA77_002022"
     CDS             16626..26117
                     /gene="irp1"
                     /locus_tag="POA77_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDI6365464.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHAMEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDQPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSNETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFNLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEITHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGRCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEIG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDTPTEPAKPTPVAGGNPA
                     LERQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLK
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26114..27214
                     /gene="ybtU"
                     /locus_tag="POA77_002023"
     CDS             26114..27214
                     /gene="ybtU"
                     /locus_tag="POA77_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDI6365465.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLNSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLL
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27211..28014
                     /gene="ybtT"
                     /locus_tag="POA77_002024"
     CDS             27211..28014
                     /gene="ybtT"
                     /locus_tag="POA77_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDI6365466.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELQENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGNHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            28018..29595
                     /gene="ybtE"
                     /locus_tag="POA77_002025"
     CDS             28018..29595
                     /gene="ybtE"
                     /locus_tag="POA77_002025"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDI6365467.1"
                     /translation="MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFSLLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDHYRHSAQ"
     gene            29726..31747
                     /gene="fyuA"
                     /locus_tag="POA77_002026"
     CDS             29726..31747
                     /gene="fyuA"
                     /locus_tag="POA77_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDI6365468.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6962 a         9628 c         8843 g         6692 t
ORIGIN      
        1 gttcagggga tgtttataag atatgaatac aggggaagtt cagggttagt ggatgtccct
       61 tgttttcttt tttgtttctg gtttgtcatt cattgcgatg acatcaagaa taggcgagtt
      121 tttcaccaga ttgtttttta tgaaaaaatc attcagtgac ttgaaataat tgtcattgct
      181 taaaaaaccg tgggcaagcg gataatcagt gtctttgatt tgctcgctac ctctggcata
      241 acgcatatcg gggtgtagga taccagccaa tacagggtat ttgattttct ggttgttgag
      301 ctgttcccaa gcgatgaggt ccatcagagg gataatcttg taatcaagta tcttctggaa
      361 tgttttatga ccaaaccgga aatctttttc cggtggttta ggcactttca ggtgcttttg
      421 ccactgactg ataagcactt tcaggtgttc agccagatcg tcactgctgg cttcttccaa
      481 gtcaatttca atcagcatct tgttcgctgg gttcagacct ccctgtttca ggtattcaga
      541 tatgtaatcc gaactcacag aaacattttc ttcttgaggc gtattctcca gccgatacat
      601 tgcccgtcca tagttaaaca gtgagttcct tgtcattggc ctgacagcat agctatgaga
      661 cagcatgtgg ctttcagaga tccatttatc ctccagcaag accctgccag agtggatcat
      721 cgcatcatgg tgaataacgg cattacgatg cttatcgtcc agtgtttccc attgctgtct
      781 ggctttatag acaaacatgc gcctttccag ctccgcataa atttgttcca gagtgaggtc
      841 tttcaacgca tcatagcggc taacatcaaa ccatttgctt aatgcttcta ctatctccgc
      901 gacaggtatt ctttttgtca ttcttttccc taaaaatgtt gatgttcatt acattgacgt
      961 taacgcatcc ggcagcatag agaaagggaa taatgatagc tggcgctctc agcatcagtt
     1021 atagagtgtt cttctgtctt tagttatctt tccgggggta aacatatcgg gtaatagcag
     1081 taatctgttg aatcagtgat tgaagatttg tagcagaaca gggagatcgt tcagtgaggt
     1141 tcagtgaagc gtgttgtcat aactaattga tagtaattga ctttatggtg atatggcatt
     1201 aaaaatgctg gtaaatatga tggtaaaaac agacgccatt ttttgaaagc cactaacagc
     1261 aggagataca gatgaagttc gagtccagtc agaggagcca atttgctgtt ttcatgcatc
     1321 cttgcgaatc attatatgat gttggttcaa caggttagcg tgaaaactct tcccggtgca
     1381 ttttgatttt accctccgca tcgggaaaaa ttggtggtca aatctggggt caggttagtt
     1441 cgataatgga gcgaccccca tatgtccctt accgacgcga aaatccgcac tctcaaacct
     1501 tctgataaac cctttaaagt ctccgattcc cacggtctgt atctgctggt caagccgggt
     1561 ggctcacgtc actggtatct caagtaccgt attaacggta aagaatcgcg cattgcgctg
     1621 ggtgcctatc ctgccgtctc cctctctgat gcgcgacagc agcgtgaagg tgtccgtaaa
     1681 atgctggcgc tgaatatcaa cccggtacag cagcgggctg ctgaacgtgg ctcacgaaca
     1741 ccggataaag tttttaaaaa cgtggcgctg gcgtggcata aaagtaaccg gaaatggtcg
     1801 cagaacaccg ctgaccgtct gcttgccagc ctgaacaatc acatctttcc ggtcattggc
     1861 aacctgcctg tatcagaact taaaccccgt catttcattg acctgctgaa aaggatcgag
     1921 gaaaaaggtc tgctggaagt tgcgtcccgc acgcggcagc acctgagtaa cataatgcgc
     1981 catgcggttc atcaggggtt aatcgatacg aacccggcag caaatcttgg cggcgtgacc
     2041 acacctcctg tcagacggca ctatcctgcc ctgccactgg agcggctgcc tgaactgctt
     2101 gaacgtattg gggcatatca tcagggtcgt gaactgacgc ggttggccgt cctgctgatg
     2161 ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc gctggtcaga gattgatttc
     2221 acaaaccgag tctggacgat acccgcgacg cgagaaccca ttattggagt gcgttattcc
     2281 ggccgcgggg ccaaaatgcg aatgtcgcat atcgtccccc tctcagaaca gtccatcgcc
     2341 attctgaaac agattaagga tatcaccggt aataatgaac tgatcttccc cggcgaccat
     2401 aacccgtata agccaatgtg tgaaaacacg gtcaataagg cactgcgggt gatgggctac
     2461 gacacgaaaa aggatatctg cggtcacggc ttccgggcaa tggcatgcag tgcgctgatg
     2521 gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga tgagtcatca ggagcgcaat
     2581 accgtgcgca tggcttatat tcataaagca gagcacctag aagcccgcaa agcgatgatg
     2641 cagtggtggt cggattatct ggaagcatgc cgagaatctt atgcaccgcc ttatacaatt
     2701 ggtaaaaata agtttatccc atagtatact aacgaatccc acactctggc aaattaccac
     2761 cacctctaca ggaggtggtt ctatatagac ttttatcaac ttcatgacga aaaacaccta
     2821 ttttatccaa aaagaagaca atagagtcac agaggaatca aagagcgact caattgtctt
     2881 tggacagtct ggttgtgagg ttagaaaaca gttactccta caccattaaa tagggcgcaa
     2941 tgctcgctaa tttctcccgg gtttccgtca gttcgcgttc cggtgtagat tgcgcgatga
     3001 ttcccgcccc cgcttgtatc cagcagcgct ggctgtcctg aaatacggaa cgcagaacta
     3061 gcgccgcatc gaagcgcata tcgtccagca gcagaattgc gccggaatag agctctcgcg
     3121 gcgtcttctc aatttgcata atcgcgttca gggcagcgtt tttagggata cctgaggcgg
     3181 taatcgacgg aaacagcacg gtaaacgcat cccaggcatc cttgttttcc gcaagctgac
     3241 cgctcacgcc ggaccccaga tgctgaacgc tgccgcgctg gcgaaccgac attaaatctt
     3301 caaccaccac gctgcccggc aggcaaacgg cctccagttc agcaatcgct tctttgacag
     3361 aaaggatatg ctcaagcacc tctttactgt cgtgcagcag ttctgcctct ttcgccttat
     3421 tatgctccgg gtttcccatg cgatcgcggg tgcccgcaag cggttcagtg accactttat
     3481 tgcccgtcac tgacatcacc agttccgggc taaagcccag cgcttcgcgt ccttcctgac
     3541 ggaacataaa cgagcgcaca ggtgtatttg cctgccgccc gtataacagc gtggcgggca
     3601 tatcaatccg cgatggcagg ggaatagcgc gcgagacaat cactttgaca tactcgccac
     3661 ggcggatctc cgcaacggcg cgtgcaacct gttgtttata cgcctcgcca ttgagggcgg
     3721 tatccaccgc cagtggtgcg ttctgcgtcg ttgtactggc ctcttttacc cactcacaca
     3781 aacgtcggcc cccgtcggcg gagtccgcat aaacggtgac atttcccttt tcaaaaataa
     3841 gttcttcacg gggaacggtt aacgtcagca gcggccactc cccggcgtta aaggcaatcc
     3901 cccgcgcatg cgccgcaaag ttgaacccaa cctgtccata aacccgacgt cctttcacct
     3961 gaggatgtgc cataaaacga cgcgcgcagt cggtaatgga atccaccgcc cattgttgct
     4021 ccccggcatc atcaataaat gtcgcctgcg ttccgtcggc attaatgtgc aaccgtgcca
     4081 ggcacccttt gcctaaatac caacagggtt gacgctcata gacataacac tcttcttctg
     4141 cgaactggcg taaaacgcca gaaatcgtcg gcagccattg ttcctctggt aacgccaggt
     4201 gtaaaaattc actgattttc atcccttacc tctctgtgtt attcccggat caatcaattg
     4261 cgcttgtcat cagttctctt gccgaatgca gccggatata agcaacccac gccgccagca
     4321 acgtgaatac cccggcaaac aggaagcagg ccgcatagcc aaaatgttga gcaacaacgc
     4381 cgccgataac accagccagg atggcgattg ccgcgtcagt acattgaaag agggtgaagt
     4441 cgacaccggc ctgcaaaggc gatgaaagcg acatcagcgt ggcgtaaaga cagaccagcg
     4501 cgcaggccag cgaaatggac tggacaatga ccagacactg gagaatcggc agcaacagat
     4561 gacccggcgc catcatgaac gtcatcacca ccgccagcag cgcgatcccc tggacgccat
     4621 aagccgtcaa cagcgctctg ccgggtgagg tgtatttcat cagtaatccg ccggccagcg
     4681 ttcctgctat gcccgctgca atattgccgc cgctgaacag cgcgcccaac gcagacatgc
     4741 tcaacccatg atccaacaac agcggcgcca gaagaggcag cacaaaccgc atacctgaat
     4801 tcagcattaa taccagcagt aagcccaggc gcgcctgctt cctccttagc gcataaccta
     4861 acgccggaac atgcgggata ggcgctgttc gcgtcggctc cgtaatgcgc cacagcggga
     4921 atgacagcgc cataatcagc atcgccatca tcagcatggc gacaggccag ccggatgctg
     4981 ccgacaacat taggaacacc ccgccgccgc acatcattcc cagatagctg ccgccgacct
     5041 gcacgctgtt tccccagccg taacccgcgc gagtcagttg atcgacgcaa aagccgtcgc
     5101 tggcgatatc aatggtggaa gcgaccgtgc ctgccaccat aaacagaaca aataacgcga
     5161 cgatttgcgt atcgggaaat cctccttctc gcccaaacca gccaatcgct gcggcaatca
     5221 tcaggatcgc cgctagcgcg acctgaccac gcaggattaa catccgcgaa cggcgttcct
     5281 ggctaccggg cggaagccgc cagcgttcga tccacggcgc ccaaataaac ttcagcgccc
     5341 agggcagcat gaacagggtt gtcgcaccgg caagcgccag cgatccgcca gcagcgcgca
     5401 ccagcgcggg taaggactgc atagacagcg ccgataccag actctgcgtc acatagacgc
     5461 cggcaatagc aaaaatcacc cgcccggtcg tcaacgtcag cggtttcaca ttcgactgaa
     5521 catcactcat gcacccactc tccagacgcg gaaaccccgt cgtcgcgcca cacgcgcgcg
     5581 gccatttgcg cctgccacag cgcctgataa cgtccatgat gtgagagcaa ttgtgcatga
     5641 gtaccctgct caaccacctg tccctcttcc atcaccagaa tgtttccggc cccggcgatg
     5701 gtggataaac ggtgagcgat gataatcacc gtccggttgt gaaccaggtt atcgatcgct
     5761 ttttgcaccg ccagctcgct ttcaatatcc agcgcggcag tcggttcatc gagaatgacg
     5821 accggcgcgt ttttcaataa cgctctggca atggaaatcc gctggcgctc gccgcccgat
     5881 agctggccgc ccatctctcc cattggcgtc agccagcctt gcggaagacg ggaaataaac
     5941 tcaaggcact gcgccgcgcg ggcggcctct tctacctcct gccgcgtcgc ctgtgggcgc
     6001 gcgatacgga tattcgccag cagcgtgtca tcaaacagcc agacatcctg gaaaacaaca
     6061 gaaatcaggc tattgagctg ttccggcgtc aggcggcgaa tatcgacgcc gccaatagaa
     6121 atctgccctt gctgcggatc ggcatagcgc attaacagtt tggtgaccgt agttttgcct
     6181 gcgccggagg cacccaccag cgcgctcata ctggctgccg ggaacgtcaa agaaacatga
     6241 ttaagcgcgt ggccgtcgcc ttcttcgtag cgatagctga cgttgtcaaa gcggatatcg
     6301 taacgttccg gcatctcgct ttgctctgcg acgggaagcg gtgctatcgc cataaaccgc
     6361 tcaatccgtt gcagggcgct ggcgatcagt tccacaaccg aggtgtagct gataaacatc
     6421 gccatcggtt ctgcgaagcg cataatcatc gcgacggcgg caatcaaaaa ggcgaggttc
     6481 agagtgcccg tcactaccca gacgatcccg gataacacca ccacctgtag gcccaactcc
     6541 acgacgctgg cgatcagcat cgtcgcgcca gcgccctgac ggtgagtgcg ggtctgtaag
     6601 ttttccagcg cattgaaatg cgccagcagc gcccggcttt tatcggcatc gctgccgcag
     6661 gtgcgcaata ccatcatccc ctgagcaaat tcaacgatat cgccgctcag acgctggtgc
     6721 gcttccccca gcgtctgcat ttgtcgccgc atcgccgggc ggcgccagta ataaaacggc
     6781 accagcagag ggaagatcag caacatcacc agccccagcc gccagtctat ccacaatgtc
     6841 gccagcgacg ccgtcagcgg cgtgacaatg gtgagcagca aaatattggc tatcgcaata
     6901 acataattga ggttttcatc cacgctgccc agcagcaagg cgttcatttc acccgccctg
     6961 ccgcgctgga gcttctccag cggcacgcgg cgtaactgct cgccaagttg caggcgcaac
     7021 tcatgggtgg cctgcgccag atgaccacgg tattcaaagc ccaggccata ccagcgtagc
     7081 accagcgtca caattgcggc gacgctgaag gccatagccc agttaagaag ttgcggcgcg
     7141 tctccccgta acagcgcatc aatgatcgga taaagacagg caaaggcgat cccctgcatg
     7201 aatgccgcca gcaacagcgc cagcatactg cgtcgcagca tcctggcctg actgccaacg
     7261 ctggagataa gctggcgcca gttgacgcgc caggccaggt tatccgcagg attattgtct
     7321 ttcattttcc accacctctt cctgtgttcc acccggcacc caatgctgcg cctgctgata
     7381 gtgttgccag agccgttgat acaggccgcc ctgcgccaac agttgcgtat ggttccccat
     7441 ttccctgagc tgtccgtcgg aaaacagcaa tatcacatcg gcctgagtca ccattgagag
     7501 acgatgcgcg accatgatga ccgtccggcc acgcatggcc gccgcgagcg ccttgataag
     7561 cgccgcttcg ttttccgggt cagcaaacgc cgtcgcctca tcgagcacca ggatggggcg
     7621 atcctgcaaa agcgcccggg cgatagtaat gcgctgccgc tggccgccgg agagaaatac
     7681 cccacgttcc ccgactcgtg tgttgtaacc ttctggcaga gcgctaataa aatcatgggc
     7741 ctgcgccact ctggccgccg ctattaccgc ctccagcggc gtatccggcg cgcccagacg
     7801 aatgttattg gctatcgtgt cggcaaaaag gaagttgtcc tgagacacaa acgagagttg
     7861 cttcatcagg gtgtccgtct gcatatcacg cagatccacg cctccaatac ggatatggcc
     7921 tttgtccggg tcggcgtaac gcagcagcaa gcgcgccacg gtgctttttc cggcgccgct
     7981 tggcccgacc agcgccacaa tttgcccggc aggcacatga aagctcacct cctgcaacgc
     8041 ggcgccagta cgcgcttgcg gataatgaaa gctcacctgc tcaaaggtaa tgctcgcctc
     8101 ttgcggctgc tggtcagact gcggcagcga taactccggc atcgcgagaa cctgataaat
     8161 acgctgaatg cttaaacgcg tttgcgcgac caggttattg agcatcatca ttggcattac
     8221 ggcttcggcc attccgctgc ccagcagtaa aacggccacc cacgcgataa aatcgaaact
     8281 gccatagtgc agcaggccgt atcccgacca gatcaggaca aacagggtcg ggagaggatt
     8341 cagaatcgag aaggaaaaac gcgctgaaaa accggctttg cgataccagg ttttgagcac
     8401 atcgacccac tcttcaaggg ctcgttgata gcgcaaaaaa ctggtgctgc cgctatcaaa
     8461 cgttctcacc acgggcatgg cctgcacgaa ctcaatcacc gccgcgctaa cctgctcgcg
     8521 cgccttatga taacgctgag ccatatcttc cgaacgctgg cgggcgagca cgagaacaac
     8581 cgatccaaac gccagtaccc ccagcgtcgc gattgccagc cgccagtcca gccagaacaa
     8641 gataacgatt gtcgccagcg gcatgatgat cgcgcgggca tagagcggcg tgctgtcggc
     8701 cacaaaaata tgtaacgact tcacgtcatc cagcatcact ttcgccactg acccgctgcc
     8761 catttgctgt aacacgccag gcggaagctg caatgcttta cgggctaacc tgctgcgcag
     8821 aatgttctcc agatgaaaag ccgcgtaatg agaggtgttg aacgcctgta agcgcagaac
     8881 aaaagttaag accacgcagc cgatcacgcc gcccagcggc caggccggaa tagcgtcagg
     8941 cgtcgcgcga atgtcacgca ggctccacgc tagaaaaagg agcgccccca ggctggttaa
     9001 tgccgccagc cccgccagcg ccatagctaa aataaccttc ctgcgaaccg gtgtaatcac
     9061 ctgccagaga ggaaatcggg acagcgattc agtattggat gattgagatg acatgggagt
     9121 aactgaattt cctgatgaat ttttacggac gccaaaaacg acaattcagg gtggcgttct
     9181 gagaattaat gtgaataata accattatca ataactcatc taccccattc ggttctattt
     9241 atacccgcat tggtctaagc cacagggaga taaccaggtc atgacggagt caccgcaaac
     9301 gcaatctgaa atctctattc accagttggt ggtcgggaaa cctgcaaacg atggcaatat
     9361 tcctgctcag tgtgaactgc tgcgctgttc gttgcaggaa ggtatggata ttttgctctg
     9421 gaggggccat tttgcgcggc ctgagacgtt gcagctacac gacgatctgg gcaggattaa
     9481 cttctcgtgc atcctggaag gcacatcgcg ctttgcgatt cagggactcc ggcgccacac
     9541 cgactgggag cttgcgcgca atcgccatta cattacccat acgccagact gtcgcggcag
     9601 cgcctcctat tgtggccgat tcgagagcat taccctctcc ttcagccctg caaccctcgc
     9661 gttgtgggtg ccggatatta gcgcggacat taagaataag atcgactccc actgctgttg
     9721 ccagcagcag cgttgtaatg ctgaaaccca cttaacggct caggccttgc gtcacgcgct
     9781 gatgcgcatg cacggcggct tcagtcatga acaaaagccg tcgacgctat ggttgcaggg
     9841 gcaaagtctg gtcatgctca gtctggtgct ggatgaacat cgcgaagacg ccagttgcct
     9901 ctcctgccac ttcaacccga tggaacgcca gaaactgctg cgggcgaagg atctgctgct
     9961 ggctgatctg acgcaagcgc cgggcgtcgc ggagctggcc agagagtccg gtctgagcgt
    10021 tctcaaaatt aagcgaggct tccgcgttct gttcaacaac agcgtctatg gcctgtttca
    10081 ggctgaacga atgcaggaag ccagaagacg cctggccaac ggcaatacct ccgtcatgac
    10141 ggtggcggcc gatctgggct atgccaacgc cagccatttc tctgccgcct tccagaaaca
    10201 gtttggcgta accccttcga cctttaaacg cgtgatgtaa ccggggtcgc gcccccctaa
    10261 actcctccct gacagaggcc cggatacccc atccgggcct ctgttctttc cgcctgttac
    10321 caacccgaaa cgggcataaa taaacccgtt tcgggtagca tccaattgtt aataataatt
    10381 attctcatat gagcaatgct tttcggtaag acgtgccatc aggaggaaga atgatttctg
    10441 gcgcaccatc tcaggattcg ctgttaccgg acaaccgcca cgcggctgat taccaacaat
    10501 tacgcgagcg gctcatacag gaactgaatt taacgccgca gcagttacat gaagagagca
    10561 acctgatcca ggccggcctg gattccataa gattgatgag atggttacac tggtttcgta
    10621 aaaatggcta ccgccttacc cttcgcgagc tgtatgccgc ccccacgctg gcggcatgga
    10681 accagttaat gctcagccgg tcgcctgaga acgcggaaga agaaacgccg cccgacgaat
    10741 catcctggcc gaacatgacc gaaagtaccc ccttcccatt gacgccagta cagcacgcct
    10801 acctgacggg ccgcatgccg gggcagacgc ttggcggcgt gggttgccac ctgtatcagg
    10861 agtttgaagg ccattgtctg acggcgtcgc agctggagca ggccatcacg accttgctgc
    10921 aacgccaccc aatgctgcat atcgcctttc gccccgacgg gcagcaggtc tggctaccgc
    10981 aaccttactg gaacggcgtc accgttcatg atttacgcca taacgacgct gaaagccgcc
    11041 aggcctatct ggacgcactg cgccagcgcc tgagccaccg tcttttacgc gtggaaatcg
    11101 gcgaaacgtt tgattttcag ctgacgctct tgccggacaa tcgccaccgc ctccatgtca
    11161 atattgacct gctgattatg gatgcctcca gctttacgct tttcttcgat gagcttaacg
    11221 ccctgctggc cggagaatcg ctgccggcta tcgacacccg ctatgatttc cgctcgtatt
    11281 tgctgcacca gcagaagatc aatcaaccac tgagagacga cgcgcgcgct tactggctgg
    11341 cgaaagcatc gacgcttccc cccgcgcccg tcttgccgct ggcctgcgaa cccgccacgc
    11401 tacgtgaagt ccgtaatacc cgacgccgca tgattgtccc ggcaacacgc tggcacgcct
    11461 ttagcaaccg ggccggcgag tatggcgtga cgccgacaat ggcgctggcg acctgttttt
    11521 ctgccgtgct ggctcgctgg ggcggcctga cgcgactgct gcttaacatc accttattcg
    11581 accgccagcc gctgcacccg gcggttggcg cgatgcttgc cgacttcacc aatattcttc
    11641 tgctggatac cgcctgcgat ggcgataccg tcagcaacct ggcgcgtaaa aaccagctca
    11701 cgtttacgga ggactgggag catcgccact ggtccggcgt cgaattactc cgtgaactca
    11761 aacgccagca gcgctacccc cacggcgccc cggtggtatt taccagcaat ctggggcgtt
    11821 ccctctacag cagccgcgca gaatcgccgt tgggcgagcc ggaatggggc atctcgcaaa
    11881 cgccgcaggt ctggatagat catctggcgt tcgagcatca cggcgaggtc tggctacaat
    11941 gggacagcaa cgacgcgctg ttccctccgg cgttagtcga aacattgttc gacgcctact
    12001 gccagttgat taaccaactc tgcgatgacg aaagcgcctg gcaaaagccg ttcgcagata
    12061 tgatgcccgc cagccagcgc gcgatacgcg aacgggtcaa cgccaccggc gcccccattc
    12121 ccgaaggctt gctgcatgaa ggcattttcc gtatcgctct gcaacagccg caggcgctgg
    12181 cggtaacgga catgcgttat cagtggaatt atcatgagct gacagactat gcccgccgtt
    12241 gcgcgggcag gttaatcgag tgcggggttc agcccggcga taatgtggct atcacgatgt
    12301 cgaaaggcgc aggacaactt gttgcggttc tggccgtcct gctggccggg gcggtttacg
    12361 ttccggtttc gctggatcag cctgccgcac ggcgcgagaa aatctacgct gacgccagcg
    12421 tccggctggt gctcatttgt cagcacgacg ccagcgccgg gtcagacgat attcccgtcc
    12481 ttgcctggca gcaggccatt gaggcggagc cgatcgccaa cccggtagta cgcgccccca
    12541 cgcaaccggc ctacattatc tacacctccg gctctaccgg tacgccgaaa ggggtagtca
    12601 tttctcaccg gggagcgctt aacacctgtt gcgatatcaa tacccgctat caggttggcc
    12661 cgcatgacag ggtgctggcc ctctccgccc tacattttga tttatcggtt tacgacattt
    12721 ttggcgtact gcgcgcgggc ggcgcgctgg tgatggtgat ggaaaatcaa cggcgcgatc
    12781 ctcacgcatg gtgtgagctg atccagcgcc atcaggtcac gctctggaac agcgtcccgg
    12841 cgctgttcga tatgctgctg acctggtgtg aaggtttcgc cgacgccacg ccggaaaacc
    12901 tgcgcgcagt gatgctttcc ggcgactgga tcgggcttga cctccccgcc cgttatcggg
    12961 ccttccggcc acaaggacaa tttatcgcga tgggcggcgc caccgaggcg tctatctggt
    13021 ctaacgcctg cgaaattcac gacgtccccg cccactggcg ctccatccct tacggttttc
    13081 cgctaaccaa ccaacgctac cgggtggtgg atgaacaggg ccgggactgc cctgactggg
    13141 tgccgggtga attatggatt ggcggcattg gggtcgcgga aggctatttc aacgatcccc
    13201 tgcgtagcga gcagcaattt ttgacgctcc cggacgagcg ctggtatcgc accggcgatc
    13261 tcggctgcta ctggccagat ggcacaatcg agttcctcgg tcgtcgcgac aagcaggtca
    13321 aagtcggagg atatcgcatc gagctgggcg aaatcgaaag cgcgctcagc cagctggcgg
    13381 gggtgaaaca agcaaccgtt ctggcgatcg gcgaaaaaga aaaaacgctg gcggcatacg
    13441 ttgttcctca aggcgaggct ttttgcatta ccgatcatcg gaacccggca ctgccgcagg
    13501 cgtggcacac gcttgcggga acgttgccct gttgcgccat ctcgccagag atctccgcag
    13561 aacaggtagc cgatttcctt cagcatcgcc tgctaaaact gaagccgggt cacaccgctg
    13621 gcgccgatcc tctccccctg atgaactcac tcgctatcca gccgcgctgg caggccgtgg
    13681 tggaacgctg gttagcattt ctggtgacac aacggcgact gaagcccgct gctgaaggtt
    13741 atcaggtctg tgctggtgaa gaacgcgagg atgagcaccc gcacttcagc ggacatgatt
    13801 taacgttatc gcaaattctt cgcggtgccc gtaacgaact gtcgttactg aacgacgcgc
    13861 agtggtcgcc ggaaagcctg gcctttaacc atccggccag cgccccgtat attcaggaac
    13921 tggcgacaat ttgccaacag cttgcacagc gcttacagcg cccgatacgc ctgcttgagg
    13981 tgggaacccg cactggccgc gccgcagaat cgctgttagc acagctcaac gccggacaga
    14041 ttgagtatgt cgggcttgag cagagccagg agatgctgct gagcgcccgg cagaggctcg
    14101 ccccctggcc tggcgcccgt ctgtccctct ggaatgcaga cacgcttgcg acgcacgctc
    14161 actcggcgga cattatctgg cttaataacg ccctgcatcg tctgctgccg gaagatcccg
    14221 ggctccttgc gacattacaa cagcttgccg ttcccggcgc gctgctctac gtgatggagt
    14281 ttcgccagtt aacgccgtcc gccctgctca gcacgctcct gttaaccaat gggcagccgg
    14341 aggccttgct gcataacagc gccgactggg cggcattatt tagcgcggcc gccttcaact
    14401 gtcagcatgg cgatgaggtc gcggggttac aacgcttcct cgtacaatgt cctgacaggc
    14461 aggtgcgccg cgatccccgt caacttcagg ccgccctcgc tggacgtctg ccggggtgga
    14521 tggtgccgca acggatcgtc ttcctcgacg ccttaccgct gacggctaac gggaaaattg
    14581 actaccaggc gctgaagcgt cgtcataccc ctgaagcgga aaacccggcc gaagcggatt
    14641 taccccaggg cgacattgaa aaacaggttg ccgccctctg gcagcaactc ttatcaactg
    14701 gcaatgtcac cagagaaacc gacttcttcc agcaaggcgg cgatagcctg ctggcgaccc
    14761 gtctgaccgg gcaacttcat caggcaggtt atgaagcgca attaagcgac ctgtttaatc
    14821 atccccggct ggcggatttt gccgccacac tgcggaaaac cgacgtcccg gtcgaacaac
    14881 cattcgtcca ctcccctgaa gatcgctacc agccctttgc gcttaccgac gtgcagcagg
    14941 cttacctggt ggggcgtcag ccgggctttg ccctgggcgg cgtcggctca catttctttg
    15001 ttgaatttga aattgccgat ctggacctca cccggctgga gacggtctgg aaccgattaa
    15061 tcgcccgcca cgatatgctg cgcgccatcg tgcgtgatgg acagcaacag gtgctcgaac
    15121 agacgccccc ctgggtgata cccgcacaca ccctccatac gcctgaagag gcgttgcggg
    15181 tgcgcgaaaa actggcgcat caggtactca accccgaagt gtggccggta ttcgatctcc
    15241 aggtcggata cgtggacggg atgcctgccc gcctgtggct gtgtctggat aacctgttgc
    15301 ttgacggtct cagcatgcag atcctgctgg cggagctgga gcacggctac cgctacccgc
    15361 aacagctgct tccgccgctg cccgtcacct tcagggatta tctgcaacaa ccctcgctac
    15421 agtcgcccaa tccagattct ctggcatggt ggcaggcgca gcttgatgat attcctccgg
    15481 cgcctgcgtt gccgctgcgc tgcttgcctc aggaggttga aacaccgcgc ttcgcccgcc
    15541 tgaacggcgc gctggacagc acgcgctggc atcggctgaa aaaacgggcg gctgacgccc
    15601 atctcacccc gtcggccgta ctgttgtcgg tgtggtcaac ggttctctct gcatggagtg
    15661 cacagcctga gttcacgctt aaccttacgc ttttcgacag gcgaccgctg cacccgcaaa
    15721 tcaaccagat tctgggcgat ttcacctcgc tgatgctgct gagctggcat cccggcgaaa
    15781 gctggctgca cagcgcgcag tcactacagc agcggctgag ccagaacctc aaccaccgcg
    15841 atgtgtcagc catccgcgtg atgcgtcaac tggcgcaaca gcaaaacgtg cctgccgttc
    15901 cgatgcccgt cgtctttacc agcgcgctgg gctttgagca ggataacttc ctcgcccggc
    15961 gtaatctgct caaaccggtc tggggcatct cccagacgcc gcaggtctgg ttcgatcacc
    16021 agatttatga atccgaaggc gaactgcgct ttaactggga ttttgtcgcc gcgctgtttc
    16081 ctgccgggca ggtggagcgc cagtttgaac agtattgcgc attgctaaac cgaatggccg
    16141 aggatgaaag cggctggcaa ctgccgctcg ccgcgctggt gcctcccgtt aaacacgcag
    16201 ggcaatgcgc agagcgctca ccgcgcgtat gccctgagca atctcagcca cacattgcgg
    16261 cggacgagag caccgtcagc ctgatttgcg acgccttccg cgaggtggtt ggcgagtctg
    16321 tcacgcccgc agaaaacttc tttgaggcgg gcgcaacgtc gctgaatctg gtgcaactgc
    16381 acgttttgtt acaacgtcac gaattttcca ccctgacgtt gcttgacctc ttcacccacc
    16441 cttctcctgc tgccctggcc gattatctgg ccggcgtcac cacggtggag aaaacaaaac
    16501 gacctcgccc tgttcgccgt cgtcagcggc ggatatagcg cgaagcaaac tgattttccc
    16561 cggaacgcca tcgcgaacgc atggcgttcc attgactttg tgaatcttag gaaacgggac
    16621 cgattatgga taacttgcgc ttctcttctg cgccgacagc agattccatt gatgcatcga
    16681 tcgctcaaca ctacccggac tgcgaacctg tcgcggttat cggctacgcc tgccattttc
    16741 ctgaatcgcc ggatggcgaa acgttctggc aaaatctgct ggaaggtcgt gaatgcagcc
    16801 gacgctttac gcgcgaagag cttctggccg tcggtctgga tgccgccatc attgacgatc
    16861 ctcattatgt caatatcggt acggtgttag acaacgccga ctgcttcgac gccaccctgt
    16921 ttggctattc gcgacaggaa gcggagtcga tggacccgca gcagcgccta tttttgcagg
    16981 cggtctggca tgcgatggaa catgccggtt atgcccccgg cgccgtcccc cataagaccg
    17041 gcgttttcgc ctcttcccgg atgagtacct accccggtcg cgaagcattg aacgtgacag
    17101 aagtcgcgca ggtaaaaggt ctgcaatctc tgatgggcaa tgataaagac tatattgcca
    17161 cccgcgccgc gtacaaactc aacctgcacg gcccggcgtt atcggtacag accgcctgct
    17221 ccagctcgct ggttgccgtg catctggcct gtgaaagcct gcgcgcaggc gaatccgata
    17281 tggcggttgc cggcggcgtg gcgctctctt tcccccagca ggcaggctac cgctaccagc
    17341 ccggaatgat tttctctcct gatggtcact gtcgtccctt tgacgcctcg gctgagggca
    17401 cctgggccgg taacggtctc ggctgcgtgg tgctgcgtcg cctgagagac gcgctgctgt
    17461 caggcgatcc gattatctcg gtgatcctct ccagcgcggt caacaacgac ggcaacagaa
    17521 aggtcggcta taccgcccct tccgtcgcag ggcaacaggc agtcatcgaa gaggcgttaa
    17581 tgctggcggc catcgacgac aggcaggtag gttacattga aacccacggc accggcacac
    17641 cgctgggcga cgcgattgaa attgaagcgt tacgcaacgt ctatgcgcct cgcccgcagg
    17701 atcagcgctg tgcgctcggt tccgtgaaaa gtaacatggg ccatctggat accgcggcgg
    17761 gcattgccgg actgctgaaa accgttctgg cagtcagtcg cgggcaaatt cctcccttac
    17821 tgaattttca cacccccaac ccggcgctga aacttgaaga gagccccttt accataccgg
    17881 tgtcggcaca ggcatggcag gacgaaatgc gctatgcggg cgtctcctcc tttggtattg
    17941 gcggcaccaa ctgccatatg atcgtcgcct cgctgcccga cgcgctcaac gcgcgcctcc
    18001 ccaatacgga tagcggcaga aaaagtaccg cgctgctgct cagcgccgcc agcgacagcg
    18061 cgttgcggcg gctggcgacg gattatgccg gggcgctgag agagaatgcg gatgccagct
    18121 ctctggcctt cacagccctg cacgcgcgcc gtctcgatct ccccttccgc ctggcggcgc
    18181 cattaaaccg tgaaaccgcc gaggcgctca gcgcctgggc cggtgagaaa tcgggggcgc
    18241 tggtttacag cggccacggc gccagcggca agcaggtgtg gctgtttacc ggccagggct
    18301 cgcactggcg cactatgggt caaacgatgt accagcactc aacggcgttt gccgacacgc
    18361 tggatcgctg tttttccgcc tgtagcgaaa tgctcacgcc gtcactgcgc gaagcgatgt
    18421 ttaaccccga ttcggcgcaa ctggacaata tggcctgggc gcagccggcg attgtcgcgt
    18481 ttgaaatcgc gatggcggcg cactggcgtg ctgaaggact gaagccagac ttcgccattg
    18541 ggcattccgt cggtgaattt gccgctgctg ttgtctgcgg acactatacg attgaacagg
    18601 tcatgccact ggtttgtcgg cgcggcgcgc taatgcagca gtgcgcaagc ggcgcgatgg
    18661 tggcggtatt tgcagacgaa gacacgctga tgccgctggc tcgccagttt gagctggatc
    18721 tcgccgccaa taacggtacg caacatacgg tattttccgg accggaagcc cgtctcgcag
    18781 tattttgcgc cacgctctcg cagcatgaca ttaactatcg tcgcctgagc gtaaccggtg
    18841 cggcgcactc cgctttactg gagccgatac tcgatcggtt ccaggacgcc tgcgcgggac
    18901 tgcacgcgga gccggggcaa ataccgatta tttccacgct caccgccgac gtcattgatg
    18961 agtcaacgct caaccaggcg gattactggc gccgacacat gcgccagccg gtgcgtttta
    19021 tccagagtat tcaggtggcg catcagctcg gcgcccgcgt ttttctggag atggggcccg
    19081 atgcccagtt ggttgcttgc gggcagcgcg aataccgcga taacgcatac tggatagcca
    19141 gcgcccggcg taacaaagag gcgagcgatg tcctcaatca ggccctgctc cagctttacg
    19201 ctgccggcgt cgccctaccg tgggccgacc tgctggcggg cgatggacaa cgtatcgctg
    19261 cgccatgtta tccgtttgat actgagcgtt actggaaaga gcgcgtctcc ccggcctgcg
    19321 agcctgccga cgcagcgctg tctgccgggc tggaggtggc gagtcgcgcc gcgacagcgc
    19381 tcgatctccc tcgcctggaa gcgcttaaac agtgcgccac gcgactgcac gccatctacg
    19441 tcgatcaact ggtacaacgc tgtaccggcg atgccattga gaacggcgtg gacgccatga
    19501 ccatcatgcg ccgtggacgt ctgctgcccc gctaccagca gctactccag cgcctgctga
    19561 ataactgcgt ggtcgacggc gattaccgct gcaccgacgg gcgatacgtc cgcgcccgcc
    19621 ccattgaaca tcaacagcgg gaatcactgc tgacggaact tgccggttat tgtgaaggtt
    19681 ttcaggctat tcccgacacc atcgcccgtg ccggcgatcg gttatatgaa atgatgagcg
    19741 gcgcggaaga accggtggcg attatcttcc cgcaaagcgc ctccgacggc gtggaagtgc
    19801 tgtatcagga attcagcttt ggccgctatt tcaaccaaat cgccgccggg gtattacgcg
    19861 gcattgtcca gacgcgtcag ccccgccagc cgttgcgtat tcttgaagtt ggcggcggaa
    19921 ccggcggcac caccgcgtgg ctgctgccgg aactcaacgg cgttccggca ctggagtacc
    19981 atttcaccga tatctcggcg ctgttcaccc gtcgcgccca gcagaaattc gccgactatg
    20041 attttgtgaa gtatagcgag ctggatctcg aaaaagaggc gcagtctcag ggtttccaag
    20101 cacagtctta cgatcttatc gtggcagcga acgtgattca cgccacccgc catattggcc
    20161 gcacgctcga taatctgcgc cccctgctca agccgggcgg gcgcctgctg atgcgcgaaa
    20221 tcacccagcc aatgcgtctg tttgacttcg ttttcggccc gctggttctt ccgctacagg
    20281 atctcgacgc ccgcgaaggt gagttattcc tcaccaccgc tcagtggcaa caacagtgcc
    20341 gccacgccgg attcagcaaa gtggcgtggc taccgcagga tggcagcccg accgccggga
    20401 tgagcgaaca tatcattctc gccacgctgc ccggtcaggc ggttagcgcc gtaacattca
    20461 ccgcgccatc agaacccgtg ttggggcagg cgctgacgga taacggtgat tatctcgccg
    20521 actggtctga ttgcgcaggt cagcccgaac ggtttaacgc ccgctggcag gaggcctggc
    20581 gtctgctttc acagcgtcat ggcgacgctc tgcctgtgga accgcccccc gtcgccgccc
    20641 cggagtggct ggggaaggtt cgcttaagct ggcaaaacga agccttttcc cgcggtcaga
    20701 tgcgcgttga agcccgtcat cctgctggcg agtggctgcc gctatcgccc gccgcgcctc
    20761 ttcctgcgcc gcagacgcat tatcaatggc gctggacgcc cctcaacgtc gccagcattg
    20821 accaaccgct tacctttagc ttcagcgccg gtacgcttgc gcgcagcgac gagctggcgc
    20881 aatacggcat cattcacgat ccgcacgcct cttcgcgact gatgattgtt gaggagagcg
    20941 aggatacgct ggccttagcg gagaaagtga tagcagcgct caccgccagc gcagccggat
    21001 tgattgtggt tactcgccgc gcgtggcgag tcgaggaaaa tgaagcactc tctgcatccc
    21061 atcacgcgct atgggccttg cttcgcgtcg cggccaacga acagccggaa cggttgcttg
    21121 ccgccatcga tctcgccgaa aacaccccgt gggaaacgct gcatcaaggg ttgagcgcag
    21181 tctcactatc acagcgctgg ctcgccgcac ggggtgacac cctttggctc ccttcactgg
    21241 cgcccaatac gggatgcgcc gctgaattac cggcaaacgt gtttaccggc gatagccgct
    21301 ggcatctggt gaccggagcg tttggcggat taggccgcct tgccgtgaac tggctcagag
    21361 aaaaaggggc gcgacgcatc gccctgctgg cgccgcgcgt ggatgagtca tggctacgcg
    21421 acgtggaggg cgggcagacg cgcgtctgcc gttgtgatgt gggcgatgcc gggcaactgg
    21481 ccacggttct tgacgatctg gcggccaacg gcggcattgc cggagcgatt catgccgctg
    21541 gcgtattggc tgacgcgccc ttgcaggagc ttgatgacca ccagctggct gccgttttcg
    21601 cggtaaaagc gcaggcggca agccagctgt tgcaaaccct gcgcaaccac gacggacgct
    21661 atcttattct ctactcttcc gctgccgcca ccctcggcgc gccgggtcag agcgcccatg
    21721 cgctggcctg cggctacctg gacgggctgg cccagcagtt ttccaccctt gatgcgccga
    21781 aaacgctctc tgtcgcctgg ggcgcatggg gagaaagcgg tcgggcggcc acgccggaaa
    21841 tgctggcgac gctcgccagc cgaggtatgg gcgcgttaag cgatgccgaa ggctgctggc
    21901 acctggaaca ggcggtgatg cgcggcgccc cgtggcgact ggcgatgcgc gtttttaccg
    21961 acaaaatgcc cccgttacaa caggctctgt ttaacatcag cgccacagaa aaagccgcaa
    22021 cgccggtcat tcctcctgct gatgacaacg cctttaacgg cagcctgagc aatgaaacag
    22081 cggtgatggc atggctgaaa aagcggattg cggttcagct aaggctgagc gatccggcgt
    22141 cactgcatcc aaaccaggat ctgttgcaac tcggcatgga ctcgctgctc ttccttgaac
    22201 tcagtagcga tattcagcac tacctgggcg tacgcatcaa tgcggaacgg gcgtggcagg
    22261 atctgtctcc tcatggactc acgcagctta tctgttctaa gccagaggcg acgcctgccg
    22321 cttcgcagcc ggaagtgttg cggcacgacg ccgacgagcg ttatgcgccc ttccctttga
    22381 cgcccattca gcacgcctac tggctggggc gaacccacct cattggctat ggcggcgtcg
    22441 cctgtcacgt cctgtttgag tgggataaac gccacgatga gttcaatctc gccatactgg
    22501 agaaagcatg gaaccagctc atcgcacgcc acgatatgtt gcgtatggtg gttgatgccg
    22561 acgggcagca gcgaatcctg gcgacaacgc cggagtatca catcccgcgt gacgatctgc
    22621 gcgcgctttc cccggaagaa cagcgcatcg cgctggaaaa acggcggcat gaactgagct
    22681 atcgcgtttt gcctgccgac cagtggcctc tttttgagct ggtggtcagc gaaatcgacg
    22741 attgccatta ccgtctgcat atgaacctcg accttttgca gtttgatgtg cagagtttta
    22801 aagtcatgat ggacgacctg gcgcaggtct ggcgcggtga aacgctggcg ccgctcgcta
    22861 ttaccttccg tgattatgtg atggctgaac aggcgcgccg acagacatcg gcatggcacg
    22921 atgcctggga ttactggcag gaaaaactgc cgcaactgcc cttagcgcca gagctgccgg
    22981 tggttgagac gcccccggaa acgccacact tcaccacctt caaatcgacg atcggcaaga
    23041 cagaatggca ggccgtgaaa cagcgctggc agcagcaagg cgtcacaccg tctgccgcgc
    23101 tgctcacgct gtttgccgcc acccttgagc gctggagccg taccacaaca tttacgctga
    23161 acctgacgtt cttcaatcgc cagccgatcc atccgcaaat caaccagttg attggtgatt
    23221 ttacctccgt cacgctggtt gattttaact tctcagcgcc ggtgacgttg caagagcaga
    23281 tgcaacagac ccaacagcgc ctctggcaaa acatggcgca cagtgaaatg aacggtgttg
    23341 aggtgatccg tgagctgggc cgcctgcgcg gatcacaacg tcaaccgctg atgccggtag
    23401 tgtttaccag tatgctgggg atgacgctgg aaggcatgac tatcgatcag gcgatgagcc
    23461 atctgttcgg cgaaccctgc tatgtattca cgcaaacgcc gcaggtctgg ctggatcatc
    23521 aggtcatgga gagcgacggc gagttgatgt ttagctggta ctgcatggac aacgtgctgg
    23581 aacccggcgc tgccgaggcg atgtttaatg actattgcgc catcctgcaa gccgtcatcg
    23641 ccgcccctga aagcctgaag actctcgcca gcggcatcgc cgggcacatt ccccgccgac
    23701 gctggccgct gaacgcgcag gcggactacg acctgcggga tattgagcag gcgacgctcg
    23761 aataccccgg catccggcag gccagagcgg aaataaccga acagggcgcg ttgacgctgg
    23821 atatcgtgat ggccgacgat ccgtcgccat cagcggcgat gcctgatgag cacgaactta
    23881 cccaactggc gctgccgttg cctgagcagg cgcagcttga tgagctggag gcgacctggc
    23941 gctggctgga ggcgcgtgcg ctacagggga tcgcggctac gctaaatcgt cacggcctgt
    24001 ttaccacgcc ggagatcacc catcgcttta gcgcaatagt acaggcgctg tccgcgcaag
    24061 cgtctcacca gcgtctgctg cgccagtggc tacagtgtct gacggaaaga gagtggttaa
    24121 tccgcgaagg tgaaagctgg cgctgccgca ttccgctcag cgagattcct gagcctcagg
    24181 aagcgtgccc gcaaagccaa tggagccagg cgctggcgca gtatctggaa acctgcatcg
    24241 cccggcacga cgccctcttc tccgggcggt gttctccgct ggaattgctg ttcaacgagc
    24301 agcatcgcgt taccgacgcg ctgtatcgcg acaaccccgc cagcgcctgt ctgaatcgct
    24361 ataccgcgca gattgccgcc ttgtgcagcg cagaacggat tctggagatt ggcgccggaa
    24421 ccgcagccac taccgcgccg gtgctgaagg ccacgcggaa cacgcggcag tcgtaccact
    24481 tcacggacgt ctccgcgcag ttcctcaatg acgccagagc ccgtttccat gatgaatcgc
    24541 aggtgtctta tgccttgttc gacatcaacc agccgctgga tttcaccgcc cacccggagg
    24601 cgggttacga cctgatcgtt gccgtcaatg tgctccacga cgccagccat gtcgtccaga
    24661 cgttgcgcag attaaaactg ttgctgaaag ccggcggacg tttgctgatc gttgaagcga
    24721 cggagcgaaa cagcgtattc cagctggcga gcgtgggctt tattgaggga ttaagcggat
    24781 accgcgattt ccgccgccgg gatgagaaac cgatgctcac ccgctccgca tggcaggagg
    24841 ttctcgttca ggccgggttt gcaaacgagc tggcgtggcc cgcgcaggaa tcgtcgccgc
    24901 tgcgccagca tctgctggtg gcgcgttcgc ctggcgtaaa tcgcccggat aaaaaagccg
    24961 tgagccgcta tttacagcag cgctttggca ccggtctgcc cattttacag atccggcaaa
    25021 gagaagcgtt atttacgccg ctgcatgccc cgtctgatac gccgactgag ccagccaaac
    25081 ccacgccagt tgccgggggg aatccggcgc tggaaagaca ggtggctgaa ctctggcaat
    25141 cgctgctgtc tcgccccgtg gcaaggcatc acgacttttt cgaactgggt ggcgacagcc
    25201 tgatggcgac aaggatggtc gcgcagctaa accggagagg gattgctagg gctaaccttc
    25261 aggatctgtt cagccattcg acgctgagcg acttctgcgc ccatctacag gcggctacgt
    25321 caggagagga caacccgata cccctttgcc agggcgacgg tgaggaaacc ctgtttgtct
    25381 tccacgcttc agacggcgat atcagcgcct ggctgccgct cgctagcgcg ttgaacaggc
    25441 gcgttttcgg cctgaaagca aaatcgccgc agcgctttgc cacgctcgac cagatgatcg
    25501 atgagtatgt cgggtgcatc cgtcgtcagc agcctcacgg cccttatgtg ctggcgggtt
    25561 ggtcgtatgg cgcgtttctt gcggcgggcg ccgcacagcg cctgtacgcc aaaggcgagc
    25621 aggttcggat ggtgttaatc gatcccgtgt gccgacagga tttctgttgc gaaaaccggg
    25681 cggccctgct gcgcctgtta gccgaaggac aaacgcctct ggcactgccc gaacatttcg
    25741 accagcagac gcccgacagc cagcttgccg actttatcag cctcgctaaa acggccggta
    25801 tggtgtcgca aaacctgacg ctgcaagcgg cagaaacgtg gctcgacaac atcgcgcatc
    25861 tgctgcgttt actgactgag catacgccgg gcgaaagcgt tccggtcccc tgtctcatgg
    25921 tgtatgccgc cgggagaccc gcgcgctgga cgccagcaga aaccgagtgg cagggctgga
    25981 taaacaacgc cgacgacgct gtgattgaag ccagccactg gcaaatcatg atggaagccc
    26041 cccacgttca ggcttgtgcg caacacatta cgcgctggct ttgcgcaacc tcaacgcaac
    26101 cggagaacac gttatgatgc cgtccgcctc cccaaaacaa cgcgtactga ttgtgggcgc
    26161 caaatttggc gaaatgtacc tgaatgcctt tatgcagccc ccggaggggc tggaactggt
    26221 cggcctgctg gcgcagggaa gcgcccgttc aagagagctg gctcatgcgt ttggcattcc
    26281 gctgtatacc tcgccggaac agataaccag gatgccggat atcgcctgta ttgtcgtgcg
    26341 ttcgaccgtc gccggaggaa ccggtacgca gcttgccaga cactttctga cgcgcggcgt
    26401 acacgtcatt caggagcatc ccctccaccc ggatgacatc agttccttac aaacgctggc
    26461 gcaggaacag ggctgctgct attgggtaaa cacgttttat ccgcatacgc gcgcgggacg
    26521 cacatggctt cgcgatgctc agcagctgcg tcgctgcctg gctaagacgc cgccggtagt
    26581 ccacgccacc accagccgac agttgctcaa ctccactctg gatttgttgt tgctggctct
    26641 gggcgttgat gccgccgccg tggagtgcga cgtggttggc agctttagcg attttcactg
    26701 tctgagactc ttctggccgg agggagaagc ctgcctgctg cttcagcgct atctcgatcc
    26761 tgacgatcca gatatgcata gccttatcat gcaccgcctg ctgctgggct ggccggaagg
    26821 tcatctttcg ctggaggcga gctacggccc cgtcatctgg tcatccagcc tgtttgtcgc
    26881 agaccatcag gagaacgccc acagcctcta ccgcagaccg gagatcctgc gcgatctgct
    26941 gggtctgacg cgcagcgcgg cgcccctgag ctggcgcgac tgctgcgaaa ccgtcgggcc
    27001 ggagggcgtc agctggttgc tacaccagtt gcgcagccat ctggcgggcg aacatccacc
    27061 agcggcctgc caaagcgtac atcagatcgc gttatcacgt ttatggcagc agattttacg
    27121 taagaccggc aatgcggaga tcagacgcct gacaccgccg caccacgacc ggttagccgg
    27181 tttctacaac gatgatgata aggaggcgct gtgacgcaat ctgcaatgtg catcccgctg
    27241 tggcccgccc ggaacggcaa tactgcgcat ctggtcatgt gccctttcgc tggcggcagc
    27301 agtagcgcgt ttcgccactg gcaagctgag caactggctg attgcgcgct ttctctggtg
    27361 acctggccgg ggcgcgatcg ccttcgccat ctggaaccgc tcagaagcat tacacaactg
    27421 gcggcactgc tggcgaacga gctggaagca tccgtatcgc ctgacacgcc gcttttactc
    27481 gccgggcaca gcatgggggc gcaggtggcg tttgaaacct gccgacttct ggagcaacgg
    27541 gggcttgcgc cacaaggact gattatttcc gggtgccatg ccccgcatct gcattctgaa
    27601 cgccagctca gccatcgcga tgatgccgac tttatcgctg agctgataga cattggcgga
    27661 tgttctcctg aactgcagga aaaccaggaa ttaatgtcgc tgtttcttcc tcttctgcgc
    27721 gctgattttt acgccaccga gagctatcac tacgactcgc ccgacgtctg tccgccgctg
    27781 cgcacgcctg cgctgttatt gtgcggcagc cacgatcgcg aagcctcctg gcagcaggtc
    27841 gatgcctggc gtcagtggct gagccacgtt acaggcccgg tggtgattga cggcaatcat
    27901 ttctatccca ttcaacaagc ccggtccttt tttacgcaga ttgtccgcca ttttccccac
    27961 gcattttctg caatgaccgc gttgcaaaaa cagcccagta cttcagaaag gtgacgcatg
    28021 aattcttcct ttaaatctct gattgaacag tatcccttac ccattgccga acagttgcgc
    28081 cactgggcgg cccgttatgc ctcgcgaatt gccgtcgttg atgcaaaggg gtcgttaacc
    28141 tacagcgcgc ttgatgcaca agttgacgaa cttgccgcag gtctgtcatc actgggtttg
    28201 cgttcggggg agcatgtaat tgtgcagctt cccaacgaca acgcgtttgt taccctgctg
    28261 ttctccttgt taagactggg cgttatcccc gtgctggcga tgccctcgca acgggcgctg
    28321 gatatcgacg cgctgattga gctggcgcaa cccgtcgctt acgttattca cggggaaaac
    28381 cacgcagagc tggcccgaca gatggcgcac aaacacgcct gcttgcgtca tgttctggtc
    28441 gctggagaga ccgtgagcga cgattttacg ccgctcttct cccttcacgg tgagcgacag
    28501 gcatggccgc agcctgatgt ttccgccacc gcgttgttgt tgctctcagg cggcacaacc
    28561 ggcacgccca aactcatccc gcgccgacat gccgactata gctataactt cagcgcttct
    28621 gctgaactgt gcggcatcag ccaacagagc gtgtatctcg ccgtcctccc ggtggcgcat
    28681 aactttccgc tggcctgccc cggtattctg ggaacgcttg cctgcggcgg aaaagtggtg
    28741 ctgaccgaca gcgccagctg tgatgaggtg atgcctttaa tcgcgcagga aagagtgact
    28801 cacgtcgccc tggttccggc gctggcgcaa ttatgggtgc aggccaggga gtgggaagac
    28861 agcgaccttt cgtcgctgcg cgtcattcag gcaggcggcg cccggctcga cccgacgctt
    28921 gctgagcagg ttatcgccac ctttgactgt accctgcaac aggttttcgg tatggcggaa
    28981 ggcctgctct gttttacccg gctggacgat ccgcatgcca ccattctcca cagccagggg
    29041 cgcccgttgt cccctctgga tgaaatccgc atcgttgatc aagacgagaa cgacgtcgcg
    29101 ccgggcgaaa ccgggcaatt gttaacgcgc ggcccttata ccatttcggg ctattaccgc
    29161 gcccctgccc acaacgcgca ggcctttacc gcgcaagggt tttaccgcac aggcgacaat
    29221 gtcaggctgg atgaggtagg gaacctgcac gttgagggac gcataaaaga gcagatcaac
    29281 cgcgccggag aaaaaatagc cgcggctgaa gtggaatcgg cactgctgcg tttagcggaa
    29341 gtgcaagatt gcgcggtggt cgccgcgccg gacacgctgc ttggcgagcg gatttgcgcg
    29401 tttatcatcg cgcagcaggt gccaactgac tatcagcagt tgcgtcaaca actgacccgt
    29461 atggggctca gcgcgtggaa aattcctgac caaatcgagt ttctggacca ctggccgctc
    29521 accgccgtcg gcaagataga caaaaaacgc ctgacggctc tcgccgtcga ccattatcgc
    29581 cattctgccc aataagcgca aaccgacccg aaacaggttg aaataaaccc gtttcgggta
    29641 gcaccactat tagaaatagt tatcattttc aattcaccat tgtcggtatt tttggcgttt
    29701 cgccgtctta cagggactca caacaatgaa aatgacacgg ctttatcctc tggccttggg
    29761 gggattattg ctccccgcca ttgctaatgc ccagacttca cagcaagacg aaagcacgct
    29821 ggtggttacc gccagtaaac aatcttcccg ctcggcatca gccaacaacg tctcgtctac
    29881 tgttgtcagc gcgccggaat taagcgacgc cggcgtcacc gccagcgaca aactccccag
    29941 agtcttgccc gggctcaata ttgaaaatag cggcaacatg cttttttcga cgatctcgct
    30001 acgcggcgtc tcttcagcgc aggacttcta taaccccgcc gtcaccctgt atgtcgatgg
    30061 cgtccctcag ctttccacca acaccatcca ggcgcttacc gatgtgcaaa gcgtggagtt
    30121 gctgcgaggc ccacagggaa cgttatatgg caaaagcgct cagggcggga tcatcaacat
    30181 cgtcacccag cagccggaca gcacgccgcg cggctatatt gaaggcggcg tcagtagccg
    30241 cgacagttat cgaagtaagt tcaacctgag cggccccatt caggatggcc tgctgtacgg
    30301 cagcgtcacc ctgttacgcc aggttgatga cggcgacatg attaaccccg cgacgggaag
    30361 cgatgactta ggcggcaccc gcgccagcat agggaatgtg aaactgcgtc tggcgccgga
    30421 cgatcagccc tgggaaatgg gctttgccgc ctcacgcgaa tgtacccgcg ccacccagga
    30481 cgcctatgtg ggatggaatg atattaaggg ccgtaagctg tcgatcagcg atggttcacc
    30541 agacccgtac atgcggcgct gcactgacag ccagaccctg agtgggaaat acaccaccga
    30601 tgactgggtt ttcaacctga tcagcgcctg gcagcagcag cattattcgc gcaccttccc
    30661 ttccggttcg ttaatcgtca atatgcctca gcgctggaat caggatgtgc aggagctgcg
    30721 cgccgcaacc ctgggcgatg cgcgtaccgt tgatatggtg tttgggctgt accggcagaa
    30781 cacccgcgag aagttaaatt cagcctacga catgccgaca atgccttatt taagcagtac
    30841 cggctatacc accgctgaaa cgctggccgc atacagtgac ctgacctggc atttaaccga
    30901 tcgttttgat atcggcggcg gcgtgcgctt ctcgcatgat aaatccagta cacaatatca
    30961 cggcagcatg ctcggcaacc cgtttggcga ccagggtaag agcaatgacg atcaggtgct
    31021 cgggcagcta tccgcaggct atatgctgac cgatgactgg agagtgtata cccgtgtagc
    31081 ccagggatat aaaccttccg ggtacaacat cgtgcctact gcgggtcttg atgccaaacc
    31141 gttcgtcgcc gagaaatcca tcaactatga acttggcacc cgctacgaaa ccgctgacgt
    31201 cacgctgcaa gccgcgacgt tttataccca caccaaagac atgcagcttt actctggccc
    31261 ggtcgggatg cagacattaa gcaatgcggg taaagccgac gccaccggcg ttgagcttga
    31321 agcgaagtgg cggtttgcgc caggctggtc atgggatatc aatggcaacg tgatccgttc
    31381 cgaattcacc aatgacagtg agttgtatca cggtaaccgg gtgccgttcg taccacgtta
    31441 tggcgcggga agcagcgtga acggcgtgat tgatacgcgc tatggcgcac tgatgccccg
    31501 actggcggtt aatctggtcg ggccgcatta tttcgatggc gacaaccagt tgcggcaagg
    31561 cacctatgcc accctggaca gcagcctggg ctggcaggcg actgaacgga tgaacatttc
    31621 cgtctatgtc gataacctgt tcgaccgtcg ttaccgtacc tatggctaca tgaacggcag
    31681 cagcgccgtc gcgcaggtca atatgggtcg caccgtcggt atcaatacgc gaattgattt
    31741 cttctgatta ttgtaaaagg gataccgaaa aggtatccct tttacaccac tagttaaaac
    31801 cagtaactca gcagagtcgc aaaaaatatt aatccatagt gattatttaa acaatgaaat
    31861 tgcgattagg acaaatagat ttaactttct cgttcctttc tctccttata ctaaagaaat
    31921 aatcatatca aaataaaaat tcacaacagt gcaacattaa aaacacaacc aacaaacaat
    31981 cctatataca aggcacatct ccagaatata aaagcacaga caaacaacct aaaaaaacaa
    32041 cccgaattaa taaaaccttt acaattacac accctcaact caaaacaatt tcgaaaactc
    32101 aaagatttca tcggcaaaaa cagtc
//
LOCUS       DAMZII010000045        32055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZII010000045 DAMZII010000000
VERSION     DAMZII010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.045"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(40..927)
                     /gene="rarD"
                     /locus_tag="POA77_002027"
     CDS             complement(40..927)
                     /gene="rarD"
                     /locus_tag="POA77_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI6365469.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(979..1446)
                     /locus_tag="POA77_002028"
     CDS             complement(979..1446)
                     /locus_tag="POA77_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6365470.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNHALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1611..2480
                     /gene="pldA"
                     /locus_tag="POA77_002029"
     CDS             1611..2480
                     /gene="pldA"
                     /locus_tag="POA77_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDI6365471.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2613..4442
                     /gene="recQ"
                     /locus_tag="POA77_002030"
     CDS             2613..4442
                     /gene="recQ"
                     /locus_tag="POA77_002030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI6365472.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4506..5126
                     /gene="rhtC"
                     /locus_tag="POA77_002031"
     CDS             4506..5126
                     /gene="rhtC"
                     /locus_tag="POA77_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDI6365473.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5188..5808)
                     /gene="rhtB"
                     /locus_tag="POA77_002032"
     CDS             complement(5188..5808)
                     /gene="rhtB"
                     /locus_tag="POA77_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDI6365474.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5920..6942
                     /gene="pldB"
                     /locus_tag="POA77_002033"
     CDS             5920..6942
                     /gene="pldB"
                     /locus_tag="POA77_002033"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDI6365475.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6950..7750
                     /gene="yigL"
                     /locus_tag="POA77_002034"
     CDS             6950..7750
                     /gene="yigL"
                     /locus_tag="POA77_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDI6365476.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7826..8725
                     /gene="bioP"
                     /locus_tag="POA77_002035"
     CDS             7826..8725
                     /gene="bioP"
                     /locus_tag="POA77_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDI6365477.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8613..9566)
                     /gene="metR"
                     /locus_tag="POA77_002036"
     CDS             complement(8613..9566)
                     /gene="metR"
                     /locus_tag="POA77_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDI6365478.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9689..11950
                     /gene="metE"
                     /locus_tag="POA77_002037"
     CDS             9689..11950
                     /gene="metE"
                     /locus_tag="POA77_002037"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI6365479.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11990..12805)
                     /locus_tag="POA77_002038"
     CDS             complement(11990..12805)
                     /locus_tag="POA77_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI6365480.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13067..13828
                     /gene="udp"
                     /locus_tag="POA77_002039"
     CDS             13067..13828
                     /gene="udp"
                     /locus_tag="POA77_002039"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDI6365481.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13968..15395
                     /gene="rmuC"
                     /locus_tag="POA77_002040"
     CDS             13968..15395
                     /gene="rmuC"
                     /locus_tag="POA77_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDI6365482.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15490..16245
                     /gene="ubiE"
                     /locus_tag="POA77_002041"
     CDS             15490..16245
                     /gene="ubiE"
                     /locus_tag="POA77_002041"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDI6365483.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16259..16864
                     /gene="ubiJ"
                     /locus_tag="POA77_002042"
     CDS             16259..16864
                     /gene="ubiJ"
                     /locus_tag="POA77_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDI6365484.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16861..18501
                     /gene="ubiB"
                     /locus_tag="POA77_002043"
     CDS             16861..18501
                     /gene="ubiB"
                     /locus_tag="POA77_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDI6365485.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18580..18849
                     /gene="tatA"
                     /locus_tag="POA77_002044"
     CDS             18580..18849
                     /gene="tatA"
                     /locus_tag="POA77_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDI6365486.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18853..19368
                     /gene="tatB"
                     /locus_tag="POA77_002045"
     CDS             18853..19368
                     /gene="tatB"
                     /locus_tag="POA77_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDI6365487.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19371..20147
                     /gene="tatC"
                     /locus_tag="POA77_002046"
     CDS             19371..20147
                     /gene="tatC"
                     /locus_tag="POA77_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDI6365488.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20189..20971
                     /gene="tatD"
                     /locus_tag="POA77_002047"
     CDS             20189..20971
                     /gene="tatD"
                     /locus_tag="POA77_002047"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDI6365489.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20968..21456)
                     /gene="rfaH"
                     /locus_tag="POA77_002048"
     CDS             complement(20968..21456)
                     /gene="rfaH"
                     /locus_tag="POA77_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDI6365490.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21623..23116
                     /gene="ubiD"
                     /locus_tag="POA77_002049"
     CDS             21623..23116
                     /gene="ubiD"
                     /locus_tag="POA77_002049"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDI6365491.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23162..23863
                     /gene="fre"
                     /locus_tag="POA77_002050"
     CDS             23162..23863
                     /gene="fre"
                     /locus_tag="POA77_002050"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDI6365492.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24055..25218)
                     /gene="fadA"
                     /locus_tag="POA77_002051"
     CDS             complement(24055..25218)
                     /gene="fadA"
                     /locus_tag="POA77_002051"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDI6365493.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25228..27417)
                     /gene="fadB"
                     /locus_tag="POA77_002052"
     CDS             complement(25228..27417)
                     /gene="fadB"
                     /locus_tag="POA77_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDI6365494.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27607..28938
                     /gene="pepQ"
                     /locus_tag="POA77_002053"
     CDS             27607..28938
                     /gene="pepQ"
                     /locus_tag="POA77_002053"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDI6365495.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28938..29552
                     /locus_tag="POA77_002054"
     CDS             28938..29552
                     /locus_tag="POA77_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDI6365496.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29591..31042
                     /gene="trkH"
                     /locus_tag="POA77_002055"
     CDS             29591..31042
                     /gene="trkH"
                     /locus_tag="POA77_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDI6365497.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31054..31599
                     /gene="hemG"
                     /locus_tag="POA77_002056"
     CDS             31054..31599
                     /gene="hemG"
                     /locus_tag="POA77_002056"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDI6365498.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            31977..>32055
                     /locus_tag="POA77_002057"
     rRNA            31977..>32055
                     /locus_tag="POA77_002057"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7467 a         8170 c         8775 g         7643 t
ORIGIN      
        1 agtgggtagg ctctttaaac ggcctaccca tgttaattct taactttttc tacgctgcgt
       61 ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggcga aagtcaccat
      121 cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg
      181 gccaatgtac tggaaaaagc ctaacgttga gagacgcaag cgcgtggcag cggcggtaaa
      241 acacaacagc ggtacggtag tgacaatacc ggcggcgatc agcagtaaat tcagcgacat
      301 cgggttttgc cccatatggc tggttgagct gtcggcaata gcaaacaggt aaattgccgc
      361 cacgggcagc agccacatgg tttcgattaa catgccggtt tgcgcttcaa cggcaatctt
      421 cttgcgtacc agaccgtaga aggcaaaact aaatgccagt cccagcgcga taataggtag
      481 cgaaccaaaa gtccacagct ggactaacac gccacatatc gccagaatca ccgccagcca
      541 ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg
      601 gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa
      661 cagtagccag ttgccaccaa tcagcacggc agagactgcc agcataaaaa ttttctgtgg
      721 cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat
      781 aaagaaaaac gaccagatca cgcgatgcgt caggatttca tcggcgggca cgtagtaaat
      841 caacttgaag tacgctggcg ctatacccca aataaaataa gcggcaagag cgagtaatac
      901 gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt
      961 ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag
     1021 ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag
     1081 gctactggta gcagtaaaac gctcgcccct gcctgggcgc agataatcaa cgcgaagatc
     1141 aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg
     1201 ggttaaggta cttcccacgc acaccagacc ggcggcgaca tccagcgccg acgcaatgac
     1261 cccgccgtgc aaaatgcttt gcgcccagtt gcccaccatc attggctgat ttttaaaggc
     1321 cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgt ggttaaatgg
     1381 catgtgataa acaaacatct cacccactaa tttcagggct tgttcagcag tcagtacggc
     1441 agacatatca tccttacact tcattggtta atgaaatgtt gattttatgc ttctttgttg
     1501 ttggtttcta ctttaggaag ggataactaa cggcaatgga gttaagtatg taaaatagcc
     1561 cgcagaaaaa cattcacctt atcaataatt cgttacggag aacacgaccg atgcggactc
     1621 tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa
     1681 cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc
     1741 aggagcatga caatccgttc acgctctatc cttatgacac caactacctc atctacacac
     1801 aaaccagcga tctgaataaa gaagcgattg ccagttacga ctgggcggaa aatgcgcgta
     1861 aggatgaagt aaagtttcag ttgagcctgg catttccact gtggcgtggg attttaggcc
     1921 cgaactcggt gttgggtgca tcttatacgc aaaaatcctg gtggcaactg tccaatagcg
     1981 aagagtcttc accgtttcgt gaaaccaact acgaaccgca attgttcctc ggttttgcca
     2041 ccgattaccg ttttgcaggt tggacgctgc gcgatgtgga gatggggtat aaccacgact
     2101 ctaacgggcg ttcagacccg acctcccgca gctggaaccg cctttatact cgcctgatgg
     2161 cagaaaacgg taactggctg gtagaagtga agccgtggta tgtggtgggt aatactgacg
     2221 ataacccgga tatcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg
     2281 gtgatgcggt gctcagtgcg aaaggacagt acaactggaa caccggctac ggcggcgcgg
     2341 agttaggctt aagttacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg
     2401 gctatggcga atcgctcatc gactataact tcaaccagac ccgtgtcggt gtgggagtta
     2461 tgctaaacga tttgttttga tgaacggttg agttgttggc aaatctggaa tccagcatcc
     2521 aggattaccc tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc
     2581 gcctgttttt atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg
     2641 agtccggagc taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgccccggcc
     2701 aggaagaaat tatcgacact gtgctttccg gtcgcgattg cctggtcgtc atgcccaccg
     2761 gtggcggaaa atccctttgc tatcaaattc ctgccttatt gctaaacggc cttaccgtgg
     2821 ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga tcaactgcaa gccaacggcg
     2881 tggcggcggc gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag
     2941 gctgccgcac cgggcaaatt cgtctgcttt atatcgcgcc ggaacgcctg atgctggata
     3001 actttcttga gcatctggcg cactggaatc cggtgttatt agccgttgat gaagcgcact
     3061 gtatctccca atggggccac gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc
     3121 agcggttccc gacgctgccg tttatggcgc tgaccgccac agccgacgac accacgcgcc
     3181 aggatatcgt gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc
     3241 gtccgaatat tcgctacatg ctgatggaga aattcaaacc gctcgatcag ttgatgcgct
     3301 acgtgcagga acagcgcggt aagtccggca ttatctactg caacagccgg gcgaaagtag
     3361 aagacaccgc tgcgcgcctg caaagcaagg gtattagcgc ggcggcctat catgccgggc
     3421 tggaaaataa tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgac ctgcaaattg
     3481 tggtggcgac ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc
     3541 actttgatat tccgcgcaat atcgaatcct attatcagga aaccggtcgc gccgggcgtg
     3601 atggcctgcc tgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc
     3661 gttgtctgga agagaagccg caggggcagt tgcaggatat cgagcgccac aaactcaatg
     3721 cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg aactattttg
     3781 gcgaagggcg tcaggagccg tgcggaaact gcgatatctg cctcgatccg ccgaaacagt
     3841 acgacggttc aaccgatgct cagattgccc tttccaccat tggtcgtgtg aatcagcggt
     3901 ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt atccgcgact
     3961 atggtcatga caaactgaaa gtctatggca tgggccgtga taaaagccat gaacattggg
     4021 tgagcgtgat ccgccagctg attcacctcg gcctggtgac gcaaaatatt gcccagcatt
     4081 ctgccctcca actgacagag gccgcgcgtc cggtgctgcg cggcgaatcc tctttgcaac
     4141 ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca
     4201 actatgatcg caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa
     4261 gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt gattgagatg gctgaacaga
     4321 tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc aagctggaac
     4381 gctttggcaa accgtttatg gcgctgattc gtgcgcatgt tgatggcgat gacgaagagt
     4441 agtcagcagc ataaaaaagt gccaggatga agactccgta aacgtttccc ccgcgagtca
     4501 aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc
     4561 ccggtcccga tttctttttt gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga
     4621 tgatgggcgt gctgggcatt acctgcggcg taatggtttg ggctgggatt gcgttgcttg
     4681 gcctgcattt gattatcgaa aaaatggcct ggctgcatac gctgattatg gttggcggtg
     4741 gcctgtatct ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcag
     4801 tttctgcacc tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt
     4861 tactgaccaa tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt
     4921 ttgtcggtga taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg
     4981 aaacgctggc gtggtttacc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg
     5041 gttatcaacg tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg
     5101 gcattcattt gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg
     5161 cgtttacgcc gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc
     5221 caccagcata aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt
     5281 aatccataga gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac
     5341 cacaatagtg gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat
     5401 gaattgcgga aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa
     5461 aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat
     5521 tgcaccagcg gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca
     5581 cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac
     5641 cagcacaata tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg
     5701 ataaccgtgg ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct
     5761 taaaatgatc gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg
     5821 gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa
     5881 aaaaaacgat tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac
     5941 tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg
     6001 cgtcagcgtg atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt
     6061 tttcgcgcac agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac
     6121 gtaaaatatg cggaactggc ctatgacctg ttccatttgg ggtttgatgt cttaatcatc
     6181 gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt
     6241 aatcgcttta atgattatgt tgatgatctg gcggcattct ggcaacagga ggtccagccc
     6301 ggtccgtggc gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta
     6361 tttctgcaac gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg
     6421 atcgtgattc gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat
     6481 ccacgtttcc gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct
     6541 atcaacgtac tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat
     6601 gacccaacga ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct
     6661 ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttgcaggct
     6721 gaagaggaac gcgtggtgga taaccgcatg catgaccgtt tttgtgaact ccgcaccgcc
     6781 gcgggccatc ctgtcgaagg aggacggccg ttggtaatta aaggtgctta ccatgagatc
     6841 ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac
     6901 aggcataact cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt
     6961 tgttgcgtct gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc
     7021 caaagaaact ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg
     7081 tcaccacgtt gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac
     7141 ctccaatggt gcgcgcgttc acgatctgga tggtaatctg atttttgctc ataacctgga
     7201 tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa
     7261 cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt
     7321 taaagaagcg gtgttcaaat atgcgctgta tgagcctgga ttactggagc cggaaggcgt
     7381 cagcaaagtg ttcttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat
     7441 taacgctcgt tggggagatc gcgtcaacgt cagtttctct accttaacct gtctggaagt
     7501 gatggcgggc ggcgtttcaa aaggccatgc gctggaagcg gtggcgaaga aactgggcta
     7561 cagcctgcaa gattgtattg cctttggcga tggaatgaac gatgccgaaa tgctgtcgat
     7621 ggcgggtaaa ggctgcatta tgggcagcgc gcatcagcgc ctgaaagatc tgcatcccga
     7681 gctggaagtg attggtacta acgccgaaga cgcagtgccg cattatctgc gtaaactcta
     7741 tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata
     7801 ctcgttaatc aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc
     7861 cttctccttt agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt
     7921 gctggtgcgc gttggcctgg cggcactcgt ttttctgccg tttctgcgta cccgtggcaa
     7981 tagcctgaaa acagtcggcc tgtatatgct ggtgggcgcg atgcagcttg gcgtgatgta
     8041 tatgctgagt ttccgcgctt atctctatct gacggtttcc gaactgctgc tgttcaccgt
     8101 actgacgccg ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg
     8161 gggatatgct tttagtgcct tgttggcggt gattggtgcc gggattattc gctatgatca
     8221 ggtcaccgac catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc
     8281 catcggcatg gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc
     8341 ctttgcgtgg ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg
     8401 aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt
     8461 ggtggcttcc gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg
     8521 aacgctggga attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat
     8581 ctggcaccaa cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc
     8641 actgtgggtg catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag
     8701 gcgtgattgc gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg
     8761 cgcacggcgg cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga
     8821 ccctggcgct caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg
     8881 gcaaccatct gaatcaacaa taaggtgtta tcgacgcttt ttagtgacgg actgacgcct
     8941 gccggctgaa gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat
     9001 agcgtctcgc tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca
     9061 ggtgctaaca ccagacgcac ttcatagtcg aacatcggcg aataatgcag accactgcgc
     9121 ggcagaatat cggacgtcat taccagatcc agctcgccct gttgcaacgc gggctgcggg
     9181 tcaaacgtca cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt
     9241 tctaacgcgg gcgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc
     9301 gtctgctgcg gttcattgca ggcttgcagt gcctggctaa tttgcggcag cacctggttt
     9361 gccagttgca acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca
     9421 aatagccgga agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc
     9481 gtctgatgca acgtcgccgc agcggctgcg agagagccgc agttccgcaa cgcttgtagc
     9541 gttttcaggt gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc
     9601 ttgaggaata tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaatact
     9661 aaaaattcca taaggggcat atcatataat gacaattctt aatcacaccc tcggtttccc
     9721 tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg cggggaactc
     9781 cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt gctcgtcact gggatcaaca
     9841 aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt gcctggtacg atcatgttct
     9901 gaccaccagt ctgctgctgg gtaatgttcc gccacgtcat cagaacaaag acggttcggt
     9961 agatatcgac accctgttcc gtattggtcg tggacgtgcg ccgactggcg aacctgcggc
    10021 ggcagcggaa atgaccaaat ggtttaacac caactatcac tacatggtgc cggaattcgt
    10081 taaaggccaa cagttcaaac tgacctggac gcagctgctg gaggaagtgg acgaggcgct
    10141 ggcgctgggc cacaacgtta agcctgtgct gctggggccg gttacttacc tgtggctggg
    10201 gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg aacgacattc tgccggttta
    10261 tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc
    10321 cgcgctggta ctggaactac cacaggcgtg gctggacgca tacaaacccg cttacgacgc
    10381 gctccaggga caggtgaaac tgctgctgac cacctatttt gaaggcgtaa cgccaaatct
    10441 cgacacgatt actgcgctgc ctgttcaggg tctgcatgtt gacctcgtac atggtaaaga
    10501 tgacgttgct gaactgcaca agcgcctgcc ttctgactgg ttgctgtctg cgggtctgat
    10561 caatggtcgt aacgtctggc gcgccgatct taccgagaaa tatgcgcaaa ttaaggacat
    10621 tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca gccccatcga
    10681 cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct tcgccctaca
    10741 aaaatgccat gaactggcac tgctgcgcga tgcgctgaac agtggtgaca cggcagctct
    10801 ggcagagtgg agcgccccga ttcaggcacg tcgtcactct acccgcgtac ataatccggc
    10861 ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga atgtctatga
    10921 agtgcgtgcc gaagcccagc gtgcgcgttt taaactgcca gcgtggccga ccaccacgat
    10981 tggttctttc ccgcaaacca cggaaattcg taccctgcgt ctggatttca aaaagggcaa
    11041 tctcgacgcc aataactacc gcacgggcat tgcggaacat atcaggcagg ccattgttga
    11101 acaggaacgt ttgggactgg atgtgctggt acatggcgag gccgagcgta atgacatggt
    11161 ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg caaaacggtt gggtacagag
    11221 ctacggttcc cgctgcgtga agccaccgat tgttattggt gacgttagtc gcccggcacc
    11281 gattaccgtg gagtgggcga agtatgcgca atcgctgacc gacaaaccgg tgaaagggat
    11341 gctgacgggg ccggtgacca tactctgctg gtcgttcccg cgtgaagatg tcagccgtga
    11401 aaccatcgcc aaacagattg cgctggcgct gcgtgatgag gtggccgatc tggaagcggc
    11461 agggattggt atcatccaga ttgacgaacc ggcgctgcgc gaaggtttac cactgcgtcg
    11521 tagcgactgg gacgcctatc tccagtgggg cgtagaggcc ttccgtatca acgccgccgt
    11581 ggcgaaagat gacacacaaa tccacactca catgtgttat tgcgagttca acgacatcat
    11641 ggattccatt gcggcgctgg atgcagacgt catcaccatc gaaacctcgc gttccgacat
    11701 ggagttgctg gagtcgttcg aagagtttga ttatccgaat gaaatcggtc caggcgtcta
    11761 tgacattcac tcgccaaacg taccgagcgt ggaatggatt gaagccctgc tgaagaaagc
    11821 ggcaaaacgc attccggcag agcgcctgtg ggtcaacccg gactgtggcc tgaaaacgcg
    11881 cggctggcca gaaacccgcg cggcactggc gaacatggtg caggcggcgc agaatttgcg
    11941 tcgggggtaa aatccaaacc gggtggtaat accacccggt cttttctcat tacagcgact
    12001 tcttcccacc atactgctta aaccattcca gcatacgctg ccagccatct ttcgcagatt
    12061 cggcatgata gctcgggcga taatcagcgt tgaatgcatg cccggcatcc gggtacacga
    12121 taatctctgc tttcgcatta gcagcccgca gcgcctggcg catggtttca acgctctcct
    12181 gcggaatgct gttatccaga ccgccatata agccgagaac cggcgcgtta agatcggttg
    12241 cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt ttgccgtacc
    12301 acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac
    12361 cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac
    12421 tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc
    12481 taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag
    12541 gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg atatgttcat
    12601 gcacgccaaa aatttcctgc actacaatga ccactggcag ttggccatcg ctttgctttg
    12661 gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca
    12721 caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgtaa
    12781 atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa
    12841 tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca
    12901 gtcctgcaga atgaagagtg atttatgtga tttgaatcac ttttggtggg taaatttatg
    12961 caacgcattt gcgccatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt
    13021 gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga
    13081 tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc
    13141 tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc
    13201 atctcaccgc gaattcacta cctggcgtgc agagctggat ggtaaacctg ttatcgtctg
    13261 ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg
    13321 cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg
    13381 tgatgtcctg gttaccacgg cgtctgtccg tctggatggc gcgagcctgc acttcgcacc
    13441 gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa
    13501 atctattggc gcgacaactc acgttggcgt gacagcttct tctgatacct tctacccagg
    13561 tcaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga
    13621 agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaactc tgctgaccat
    13681 gtgtgcaagc cagggcctgc gtgccggtat ggtggcgggc gttatcgtta accgcaccca
    13741 gcaagagatc ccgaatgctg agacgatgaa acaaaccgaa agccaagcgg tgaaaatcgt
    13801 ggtggaagcg gcgcgtcgtc tgctgtaatc ctcttctcct gtctgaaggc cgacgcgttc
    13861 ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg gacgtttgta
    13921 cagcacaatt ctatgttgtg cgggtaagta atgaggctgg aggcgttgtg gatttctcaa
    13981 tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt
    14041 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
    14101 taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgtgaattgc
    14161 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
    14221 aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga
    14281 ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgctaaccgt atttttgagc
    14341 acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc
    14401 tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac
    14461 aagaacgcca taccctgacc cacgaaattc gtaatctaca gcaactcaac gcgcaaatgg
    14521 cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga taacaaaacc caggggaact
    14581 ggggtgaagt ggtactgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
    14641 atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
    14701 tgcggttgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
    14761 aacgctattt caacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg
    14821 cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
    14881 tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
    14941 ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
    15001 cgactacgct gctggtggcg ttgcgtacta ttgccaacct gtggcgttat gagcatcaaa
    15061 gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt
    15121 tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc
    15181 aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc
    15241 gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
    15301 gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
    15361 aacgcgatga tgaatataat cagcagtcgc gctagcccat taggggtagt taagccgggt
    15421 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
    15481 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
    15541 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
    15601 gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat
    15661 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
    15721 acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
    15781 gaatccatgc tcaaaatggg acgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
    15841 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
    15901 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
    15961 cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag
    16021 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
    16081 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
    16141 caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
    16201 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
    16261 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
    16321 ctcacccgcg ctgaaaacag cccgctcgcg tctgctgggt aaagtattgc gcgtggaggt
    16381 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg
    16441 cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact
    16501 tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt gagctggaag tgcagggcga
    16561 tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga
    16621 actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg
    16681 aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat
    16741 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
    16801 ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa
    16861 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
    16921 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
    16981 ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc
    17041 ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat
    17101 ctttttccac cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt
    17161 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
    17221 tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc
    17281 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg
    17341 ccggtcatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg
    17401 ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg
    17461 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgcaatttt
    17521 gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg
    17581 atggtgatgg agcgcattta cggcattccg gtgtctgatg ttgcggcgct ggagaaaaac
    17641 ggcactaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt
    17701 cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac
    17761 ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
    17821 gataaacgct atctggcaga aaactttatc gccttcttta atcgcgacta tcgcaaagtg
    17881 gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga agagttcgaa
    17941 tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt
    18001 ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg
    18061 caactggtgt tactccagaa aaccctgctc tatgtcgaag gggtaggacg ccagctttat
    18121 ccgcagctcg atttatggaa aacagcgaag cctttcctgg agtcgtggat taaagatcag
    18181 gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
    18241 atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta tttacagcac
    18301 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
    18361 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
    18421 cctgagtggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
    18481 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg
    18541 cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
    18601 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
    18661 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
    18721 ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag
    18781 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
    18841 caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc
    18901 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt
    18961 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
    19021 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacaccc
    19081 gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac
    19141 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg
    19201 aaagataatg aagctgcgca tgagggcgta acgcctgccg ctgcacaaac gcaggccagt
    19261 tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct
    19321 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
    19381 aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca
    19441 ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
    19501 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
    19561 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
    19621 cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac
    19681 gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcat
    19741 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
    19801 gggtacaggt atccaccgac atcgccagct atttaagctt cgttatggcg ttgtttatgg
    19861 cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg atggggatta
    19921 cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg
    19981 ggatgctgct gacaccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact
    20041 gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg
    20101 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
    20161 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
    20221 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
    20281 gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
    20341 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
    20401 tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga
    20461 atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt
    20521 tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc
    20581 ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct
    20641 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcgtg gaatttatat
    20701 cggcattacc ggttgggttt gcgatgaacg acgcgggctg gagctgcggg aattgttgcc
    20761 gttgattccg gcggagaaat tgctgatcga aactgatgcg ccgtatctgc tcccgcgcga
    20821 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
    20881 acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgccaa
    20941 tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
    21001 gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg
    21061 ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc
    21121 cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat
    21181 caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg
    21241 ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattcgacaa acaggtagtt
    21301 gggaaacaat ggctcactga ctgcagtacg ttttccacgc acgatttttt ccagggtgat
    21361 catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg
    21421 cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg
    21481 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
    21541 cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
    21601 gggcgtagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt
    21661 gacgctgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcatct
    21721 ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga
    21781 aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt
    21841 ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc
    21901 gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca
    21961 gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt ggtgcgccct gccaacaaaa
    22021 aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc attatgacct gctggccgga
    22081 agatgccgcg ccgctgatta cctgggggct gacagtgacg cgcggcccgc ataaagagcg
    22141 gcagaatctg ggcatttatc gccagcagct gattggtaaa aacaaactga ttatgcgctg
    22201 gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccgggcga
    22261 acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac
    22321 ccccgttccg gatacacttt cagagtatgc gtttgccgga ttgctgcgcg gtaccaagac
    22381 cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct
    22441 ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg accacaccgg
    22501 ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga
    22561 agatgcgatt taccattcca cctataccgg gcgtccgcca gatgagcccg cggtactggg
    22621 cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa cagttcccgg aaattgtcga
    22681 tttttacctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga tcaaaaaaca
    22741 gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    22801 gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt
    22861 gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg tagaaaatac
    22921 gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct
    22981 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    23041 agatccagat gttgtcgcgc atattgacgc catctgggat gaactggcta tttttaacaa
    23101 cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg
    23161 catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg
    23221 tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt
    23281 gatggatgag cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt
    23341 tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg
    23401 catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga
    23461 tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc
    23521 gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg
    23581 gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt taaagcaccc
    23641 tggtctgcaa gtggtgccgg tggttgaaca accggaagca ggctggcgtg ggcgtactgg
    23701 taccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg atatctatat
    23761 tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc
    23821 gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc
    23881 ccctgacagg cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgtc
    23941 gcatccggca attggtgcac gacgcctgat gcgacgctgg cgcgtcttat caggcctaca
    24001 ttggtgccgg gtcggtagac cggataaggc gttcacgccg catccggcaa gtggttaaac
    24061 ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg ccagaccaaa
    24121 ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca
    24181 gcccagcgga tgacccagcg cgatcgcacc accattgagg ttgatcttct cgtcaatctg
    24241 ctccattagc ccgagatctt taatgcacgg caggatctgc gcggcaaagg cttcgttcat
    24301 ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc
    24361 cggaaccggg ccgtaaccca taatcgatgg gtcacaacca acgaccgcca tcgaacgcac
    24421 acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc
    24481 agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt ttactggatc
    24541 aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    24601 gtaattaaac tgcttcagga cgccgtcggc atcgtgacca ccagttggga tgatttcatt
    24661 tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc
    24721 atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgctg ttaagcccat
    24781 catgcccgcc gctttggcga cattgcggct caggccgggg tgaaaatcga cgccgtgact
    24841 catcggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg catcgccagt
    24901 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac
    24961 ggtaaccgcc gggacagagt gcggcacttc tgccagcagc gccgcgttac gggcgatatt
    25021 aaaaccctgc tccagcgtct gctgcacaca accccagtaa atatcgtcga gggccgccgc
    25081 ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttctgc
    25141 acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    25201 aatgacaacc tgttccattg tgactcctta agccgttttc aggtcgccaa ccggacgggc
    25261 tggctcaacc ggaggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg
    25321 cacttcatac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct
    25381 accgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta
    25441 gaccagcgcc atatccgctt ccgccggagt ggcgataatg ccttcctcca gacagcgcac
    25501 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    25561 cttcggctgg ctcacttctg ccagcaggtc ttcaacggcg gcgtcttctt ctttcttcgg
    25621 cttacctttg ctgtcttctt tataacgcca gaaaccgagg ccgttcttct gaccaaagcg
    25681 gttggcatca aacagcgcgt cgatggcatc gcggtaatct ttctgcatcc gctgcgggaa
    25741 gcctgctgcc atgacagcct gagcgtgatg cgcggtatca atgcccacga catccagcag
    25801 atatgccgga cccataggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    25861 atccgcgccg tcgcgcagca gctggctgaa accggcgaaa tacgggaaca gcacgcggtt
    25921 aacaaagaag ccggggcagt cgttaaccac aatcggcgtc ttgcccatct tgctcgccca
    25981 ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa
    26041 cggcatccgg tgtaccgggt taaaaaagtg cattccgcag aagttttccg ggcgttccag
    26101 cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatccgg
    26161 gcgtactttt tgttcggttt ctgccagtac ggcttttttc actttcgggt tttcgacaac
    26221 cgcttctacc acaacatcca cgcggtcaaa cccggcgtag tcgagcgttg ggtggattgt
    26281 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag
    26341 cagtttcgcg gcttcggtca tgccgagggt taacgacttg tcgttgatat ctttcatgac
    26401 aaccggcacg cctttccacg cagactggta agcgatgccg ccgcccataa tgcctgcacc
    26461 cagcaccgcg gcctgtttcg gggtttcaac gtctttggtg agtttcttcg ctttgccttt
    26521 tacatattga tcgttaagga aaatgccgac cagtgcgcgg gcttcgttgg tatgcgccag
    26581 cgggacaaaa cttttgtttt ccaggtttaa ggcttcttca cgaccaaaac gggccgcagc
    26641 ttcaatggtt tttactgcgg tgatgggggc cggataatgt ttccccgctg tttgtgcgac
    26701 catcccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagtggttc
    26761 cagcttcggc tgacgttttg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaac
    26821 cgcctttgcg ccttcaacca gtttttctgc tttgactacg ccatccacca gaccgatttt
    26881 cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc
    26941 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    27001 ttccggcaga ccgatgcgca gatccggcgt cgccagacga taatcggtcg ccagcacgca
    27061 ttcgcagcca ccgcccagcg catagccgtt ggcggcagca atggtcggca ccggcagatc
    27121 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    27181 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    27241 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    27301 gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag
    27361 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    27421 agtctcctga atccgcaagg tgatctggtc gtaccagatg agtcgaagtg tgtattttgt
    27481 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    27541 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    27601 taaaaaatgg aatcactggc ctcactctat aaaaatcata tagctacctt acaggaacgg
    27661 actcgcgatg cgctggcgcg cttcaagctg gatgcgttac ttattcactc cggcgaactg
    27721 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    27781 tgggtgccgg taactcaggt gccaaactgc tggctgctgg tggatggcgt gaacaagccg
    27841 aaactgtggt tctatctgcc ggttgattac tggcacaacg tcgaaccgct gccgacctct
    27901 ttctggactg aagacgtgga agtgatcgcg ctgccgaaag ccgatggcat tggtagcctg
    27961 ctgcctgctg cgcgcgggaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa
    28021 ctgggtattg aggccagcaa tatcaacccg aaaggggtga tcgactacct gcattactac
    28081 cgctccttca aaaccgagta cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc
    28141 aacggtcatc gcgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaac
    28201 attgcctatc tgaccgcgac cggtcatcgt gataccgacg taccttacag caacattgtg
    28261 gcgctcaacg aacacgctgc ggtgttgcat tacaccaaac tggaccacca ggcaccggaa
    28321 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc ggctgacctg
    28381 acccgtacct ggtcggcaaa aagtgacaac gactacgcac agctggtgaa agacgtaaat
    28441 gatgaacaac tggcgctgat cgctaccatg aaagcaggcg tcagctatgt ggattaccac
    28501 atccagttcc atcagcgcat cgccaaactg ctgcgtaaac atcaaatcat caccgatatg
    28561 agtgaagaag cgatggtcga aaacgatctc accggaccgt ttatgccgca cggtattggc
    28621 catccgctgg gcctgcaggt gcatgacgta gccggtttta tgcaggatga tagcggtacg
    28681 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc
    28741 atggtgttaa ccatcgaacc gggtatctac ttcattgaat cgctgctggc accgtggcgt
    28801 gaagggcagt tcagcaagca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    28861 ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca acgtggaaaa catgacccgg
    28921 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    28981 aagagatcaa aaagagccgc ttcataacga tgttggcgca taccgatggc gttgaggcgg
    29041 cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg
    29101 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    29161 cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc gtcggggaaa
    29221 ttaccgctgt ggtagtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga
    29281 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc
    29341 cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    29401 tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg
    29461 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa gctggcggat tttagccgtg
    29521 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    29581 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt
    29641 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    29701 cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgctgt ggtggccgaa
    29761 ccgcaaagag aaaggcgaac ttaaatcccg tgaggggttt ctgatagtgg tgctgttctg
    29821 gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    29881 gattaccgat gcgttttttg aatctttctc tggcctgacc accaccgggg ccactacgct
    29941 ggtggggctg gattcgctcc ctcacgccat cctcttttat cgccagatgc tgcaatggtt
    30001 tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg
    30061 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    30121 aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    30181 gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    30241 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg acagcccgac
    30301 tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt
    30361 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    30421 tatcggcgta cagtttacgc tggtggttat ttgtacactc gtactgtggt ttcataatgt
    30481 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtat cgatggcgac
    30541 aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    30601 tttatgttca gcttttatcg gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat
    30661 ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa
    30721 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    30781 ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    30841 cacgggcgtg gatgactttt ctgcctttgc ctcagttgtt gcgacattga ataacctggg
    30901 gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    30961 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    31021 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    31081 agggacggac aaacgcgcga gattgcctcc tacctggctt cggaactgaa agaactgggg
    31141 atccagacgg atgtcgccaa tgtgcaccgc attgaagaac cacaatggga aaattatgac
    31201 cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt
    31261 gtcaaaaagc atgcgacgcg actgaattcg atgccgagcg ccttttattc cgtaaacctg
    31321 gtggcgcgca aaccggagaa gcgtactcca cagaccaaca gctacgcgcg gaagtttctg
    31381 atgaactcgc aatggcgtcc cgatcgctgt gccgtcattg ccggggcgct gcgttaccca
    31441 cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa
    31501 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    31561 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgcg
    31621 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    31681 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    31741 ctcaacagag aaaagtaaaa aaaatgcttg actctgtagc gggaaagcgt attatgcaca
    31801 ccccgcgccg ctgagaaaaa gcgaagcggc actgctcttt aacaatttat cagacaatct
    31861 gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga cgaaaaatga ataccaagtc
    31921 tcaagagtga acacgtaatt cattacgaag tttaattctt tgagcatcaa acttttaaat
    31981 tgaagagttt gatcatggct cagattgaac gctggcggca ggcctaacac atgcaagtcg
    32041 aacggtaaca ggaag
//
LOCUS       DAMZII010000046        31985 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZII010000046 DAMZII010000000
VERSION     DAMZII010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.046"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..555
                     /gene="cmtB"
                     /locus_tag="POA77_002058"
     CDS             112..555
                     /gene="cmtB"
                     /locus_tag="POA77_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDI6365499.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            583..1971
                     /gene="cmtA"
                     /locus_tag="POA77_002059"
     CDS             583..1971
                     /gene="cmtA"
                     /locus_tag="POA77_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI6365500.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDKFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1986..3263
                     /locus_tag="POA77_002060"
     CDS             1986..3263
                     /locus_tag="POA77_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6365501.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3260..4225
                     /gene="yggF"
                     /locus_tag="POA77_002061"
     CDS             3260..4225
                     /gene="yggF"
                     /locus_tag="POA77_002061"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI6365502.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4247..4756
                     /gene="fumE"
                     /locus_tag="POA77_002062"
     CDS             4247..4756
                     /gene="fumE"
                     /locus_tag="POA77_002062"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDI6365503.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4753..5466
                     /locus_tag="POA77_002063"
     CDS             4753..5466
                     /locus_tag="POA77_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDI6365504.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5438..6115)
                     /locus_tag="POA77_002064"
     CDS             complement(5438..6115)
                     /locus_tag="POA77_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI6365505.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6109..6786)
                     /locus_tag="POA77_002065"
     CDS             complement(6109..6786)
                     /locus_tag="POA77_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI6365506.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6774..7481)
                     /locus_tag="POA77_002066"
     CDS             complement(6774..7481)
                     /locus_tag="POA77_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6365507.1"
                     /translation="MHPFTSLTLWALVACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7482..8060)
                     /locus_tag="POA77_002067"
     CDS             complement(7482..8060)
                     /locus_tag="POA77_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6365508.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8083..8514)
                     /locus_tag="POA77_002068"
     CDS             complement(8083..8514)
                     /locus_tag="POA77_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6365509.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8886..9905
                     /gene="epd"
                     /locus_tag="POA77_002069"
     CDS             8886..9905
                     /gene="epd"
                     /locus_tag="POA77_002069"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDI6365510.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9955..11118
                     /gene="pgk"
                     /locus_tag="POA77_002070"
     CDS             9955..11118
                     /gene="pgk"
                     /locus_tag="POA77_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI6365511.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11333..12412
                     /gene="fbaA"
                     /locus_tag="POA77_002071"
     CDS             11333..12412
                     /gene="fbaA"
                     /locus_tag="POA77_002071"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDI6365512.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12770..13630
                     /gene="mscS"
                     /locus_tag="POA77_002072"
     CDS             12770..13630
                     /gene="mscS"
                     /locus_tag="POA77_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDI6365513.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13769..14404
                     /gene="argO"
                     /locus_tag="POA77_002073"
     CDS             13769..14404
                     /gene="argO"
                     /locus_tag="POA77_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDI6365514.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14497..15237
                     /locus_tag="POA77_002074"
     CDS             14497..15237
                     /locus_tag="POA77_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDI6365515.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15404..16300
                     /locus_tag="POA77_002075"
     CDS             15404..16300
                     /locus_tag="POA77_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6365516.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16297..17775)
                     /gene="scpC"
                     /locus_tag="POA77_002076"
     CDS             complement(16297..17775)
                     /gene="scpC"
                     /locus_tag="POA77_002076"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDI6365517.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17799..18584)
                     /gene="scpB"
                     /locus_tag="POA77_002077"
     CDS             complement(17799..18584)
                     /gene="scpB"
                     /locus_tag="POA77_002077"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDI6365518.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18595..19590)
                     /gene="meaB"
                     /locus_tag="POA77_002078"
     CDS             complement(18595..19590)
                     /gene="meaB"
                     /locus_tag="POA77_002078"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDI6365519.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19583..21727)
                     /gene="scpA"
                     /locus_tag="POA77_002079"
     CDS             complement(19583..21727)
                     /gene="scpA"
                     /locus_tag="POA77_002079"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDI6365520.1"
                     /translation="MSNEQEWQQLANKELSRWEKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            complement(21931..22824)
                     /gene="argP"
                     /locus_tag="POA77_002080"
     CDS             complement(21931..22824)
                     /gene="argP"
                     /locus_tag="POA77_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDI6365521.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22966..23151
                     /locus_tag="POA77_002081"
     CDS             22966..23151
                     /locus_tag="POA77_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365522.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23207..23866
                     /gene="rpiA"
                     /locus_tag="POA77_002082"
     CDS             23207..23866
                     /gene="rpiA"
                     /locus_tag="POA77_002082"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDI6365523.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24122..25354
                     /gene="serA"
                     /locus_tag="POA77_002083"
     CDS             24122..25354
                     /gene="serA"
                     /locus_tag="POA77_002083"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6365524.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25583..25642
                     /gene="ibsC"
                     /locus_tag="POA77_002084"
     CDS             25583..25642
                     /gene="ibsC"
                     /locus_tag="POA77_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDI6365525.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25975..26034
                     /locus_tag="POA77_002085"
     CDS             25975..26034
                     /locus_tag="POA77_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6365526.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26135..26737)
                     /locus_tag="POA77_002086"
     CDS             complement(26135..26737)
                     /locus_tag="POA77_002086"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI6365527.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26758..26941)
                     /gene="ssrS"
                     /locus_tag="POA77_002087"
     ncRNA           complement(26758..26941)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="POA77_002087"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26983..27312)
                     /gene="zapA"
                     /locus_tag="POA77_002088"
     CDS             complement(26983..27312)
                     /gene="zapA"
                     /locus_tag="POA77_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6365528.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27480..28058
                     /gene="ygfB"
                     /locus_tag="POA77_002089"
     CDS             27480..28058
                     /gene="ygfB"
                     /locus_tag="POA77_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDI6365529.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28084..29409
                     /gene="pepP"
                     /locus_tag="POA77_002090"
     CDS             28084..29409
                     /gene="pepP"
                     /locus_tag="POA77_002090"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDI6365530.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29406..30584
                     /gene="ubiH"
                     /locus_tag="POA77_002091"
                     /gene_synonym="visB"
     CDS             29406..30584
                     /gene="ubiH"
                     /locus_tag="POA77_002091"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDI6365531.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30607..31809
                     /gene="ubiI"
                     /locus_tag="POA77_002092"
                     /gene_synonym="visC"
     CDS             30607..31809
                     /gene="ubiI"
                     /locus_tag="POA77_002092"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDI6365532.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQVSEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31883..31966)
                     /locus_tag="POA77_002093"
     CDS             complement(31883..31966)
                     /locus_tag="POA77_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365533.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7657 a         7942 c         8604 g         7782 t
ORIGIN      
        1 attcactgtt ttcaaaacat cggtttccct gctcaattgc tttcattaaa ccgcacgagt
       61 attttatttt taccgcctga cggtacccta aaaataaaat aaggataatt tatgcggctt
      121 agtgattatt ttccagaatc atcaatctca gttatacatt cagcaaaaga ttggcaggaa
      181 gctatcgatt tctcgatggt atcattgctg gataaaaact atatcagcga gaattacatt
      241 caggcaatta aagattccac cattaacaat ggcccttatt atattctcgc accaggcgtg
      301 gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa ccgggatgtc tttgacatta
      361 cttgaacaag gtgtttattt tccggggaat gacgaaccaa ttaaattact catcggactc
      421 tctgctgccg atgccgattc gcacattggc gctattcagg cgttaagtga attactgtgc
      481 gaagaagaaa tactcgaaca actcttaaca gcatcatcag aaaaacaatt agcggacatt
      541 atcagccgcg gataattact ctctcctcta caggataata ttatggaaaa caagtctgct
      601 cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa tggtcatccc caatattggt
      661 gcttttattg cctggggctt cataactgcg ttatttattc ccaccggttg gctgccgaat
      721 gaacatttcg ccaaaattgt cggcccgatg attacctatt tattgcccgt gatgattggt
      781 tctactggtg gtcatctggt cggcggtaaa cgcggggcgg tcatgggcgg aataggtact
      841 attggtgtga tcgttggcgc agagatcccg atgttccttg gctcaatgat catggggccg
      901 ctcggtgggt tggtcataaa atatgtcgat aaggcactgg aaaaacgcat acctgccggt
      961 tttgagatgg ttatcaataa cttctcatta ggtatcgcgg ggatgctcct ttgtctgctg
     1021 ggttttgaag tgatcggccc ggcggtgtta attgccaata ctttcgtcaa agagtgtatc
     1081 gaggcgctgg tacatgcggg ttatctgcct ctgctgtcag tcatcaatga accggcgaaa
     1141 gtgcttttcc ttaataatgc gatcgatcag ggcgtctatt atccgctggg aatgcaacag
     1201 gcttcggtta acggtaaatc catcttcttt atggtggcct ctaacccagg tccgggcctg
     1261 gggctgctgc tggcgtttac cttgtttggt aaagggatga gtaaacgttc tgcgcccggg
     1321 gcgatgatta ttcacttcct cggtgggatc cacgaactgt atttcccata tgtgctgatg
     1381 aagccgctga ctattattgc catgattgcg ggcggtatgt ctggtacctg gatgtttaac
     1441 ttactggacg gtggtctggt ggctggccca agtccggggt ctatctttgc ttacctggca
     1501 ctgacgccga aaggctcgtt cctggcgaca attgccggtg ttacggtagg taccctggtg
     1561 tcctttgcta ttacttcgct gatactgaag atggaaaaaa cggtggaaac ggagagcgaa
     1621 gataagtttg ctcagtcagc caatgcggtt aaggcgatga aacaagaggg tgcattctcg
     1681 ttaagcaggg ttaagcgtat cgcctttgtt tgcgatgcgg ggatgggctc cagtgcgatg
     1741 ggcgcgacca ccttccgtaa acgcctggaa aaagcggggc tggcaataga agtaaaacat
     1801 tacgcgatag aaaacgtgcc tgcggatgcc gatatcgtcg tcactcacgc cagtctggaa
     1861 gggcgcgtga aacgtgtgac ggataaaccg ctgatattga ttaataacta tattggcgat
     1921 ccaaaactcg acactttatt taatcaatta accgccgaac ataaacactg attacagagg
     1981 taaaaatgaa aaccaaagtt gctgctattt atggcaagcg ggatgtccgt ctgcgcgaat
     2041 ttgaactgcc agaaattacc gataatgaac tgttagtgag tgtaatttct gacagcgtct
     2101 gtttatcgac ctggaaagcg gcgttactcg gtagtgaaca taaacgcgta cccgacgatt
     2161 tagaaaatca tccggtcatt accgggcatg aatgtgccgg ggttattgtc gaagtgggta
     2221 aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt
     2281 taccaagcgg atattcagca ggctacagct acgaatattt tggcggtaac gccacctata
     2341 tgattattcc cgaaatcgcc attaatttgg gctgcgtatt accgtatcac ggctcttatt
     2401 ttgctgcggc gtcgctggca gagcctatgt gctgcattat tggtgcttat catgccaatt
     2461 atcacaccac gcaatatgtt tatgagcatc gcatgggcgt caaacctggc ggcaatattg
     2521 cactgctggc gtgtgcgggt ccgatgggca ttggcgctat cgattacgcc attaacggtg
     2581 gcatacaacc gtcacgggtg gtggtggtcg atatcgacga caaacgcctg gcgcaggtgc
     2641 agaagctgct gccggtggat ctggcggcca gtaaaggcat tgagctggtg tatgtgaata
     2701 ccaaagggat gagcgatccg gtccagacgc tgcgggcgct gacaggagat gtcgggttcg
     2761 atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga gatggctgac gaattactgg
     2821 cggaagatgg ctgtctgaac ttctttgccg ggccgacgga taaaaacttc aaagtgccgt
     2881 ttaatttcta caacgtccat tacaacagca cgcacgtcgt cggtacatcc ggcggttcaa
     2941 cggacgacat gaaagaggcg attgcgttaa gcgccacggg gcagttgcag ccgtccttta
     3001 tggtcacgca tatcggtggg ctggatgcgg tgccagatac cgtgctcaat ctgccggata
     3061 tccctggcgg taaaaaactc atttataacg gcgtgaccat gccgctcacc gccattgccg
     3121 attttgccga aaaaggcaaa accgatccgc tgtttaaaga gctggcgcgg ctggttgagg
     3181 aaacgcacgg catctggaat gagcaggccg agaaatatct gctggcgcaa tttggcgttg
     3241 atattgggga ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca
     3301 ggcagcgttg gctgcctggc cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct
     3361 ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct tttcgtggac gagtggttat
     3421 cggcgaagga gaaattgacc atgcgccgat gctgtggatt ggcgaagagg tgggcaaagg
     3481 cgatgggcca gaagtcgata tcgcggttga cccgattgaa ggcacgcgga tggtagcgat
     3541 ggggcagagc aatgcgctgg cggtcatggc tttcgcccct cgagatagcc tgttacatgc
     3601 gcccgatatg tatatgaaaa agctggttgt taatcgactg gctgccgggg cgattgatct
     3661 gtctttgcct ctggcggaca acttgcgcaa cgtggcgcgg gcgttaggca aaccgctgga
     3721 caagctgcgc atggttacgc tggataaacc gcgcctgagt gccgcgattg aagaagcaac
     3781 acagctgggc gtgaaggttt tcgctctgcc ggatggcgat gtcgccgcca gcgtgctgac
     3841 ctgctggcag gataatcctt acgacgtgat gtacaccatc ggcggtgcgc cagaaggcgt
     3901 gatttctgcc tgtgccgtta aagcattagg tggggatatg caggccgagc tgattgactt
     3961 ttgtcaggct aaaggggatt acacggaaaa tcggcagatt gcagagcagg agcgcaagcg
     4021 ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg ctcgatgaac tggtgagggg
     4081 gaacgatatc ctctttagcg ctacgggcgt gacgggcggc gagttggtga acggtatcca
     4141 acagacggcg aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg
     4201 taatataata gactccctgc attgatggtg atctatcgga agaccaatgg cgacgctgac
     4261 agaagatgat gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac
     4321 tgcgcattct attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa
     4381 cgatgaagag atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga
     4441 cgatattgat gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta
     4501 tgccgatatc acgcactttt cccagtactg gcattacctg aacgaacagg atgaaacgcc
     4561 cggatttgcc gatgacatga cctgggattt tatcagcaac gtcaatagca taacctgcaa
     4621 tgcgacgctc tatgacgcgt taaaagcgat gaagttcgcc gatttcgccg tctggtcaga
     4681 ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa
     4741 ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc tgaacgtgca agcgcagtac
     4801 catgatgaag agatagaacg cgtgcataaa ccgttgctgc gtatgctggc ggccttacag
     4861 acggtaagtc cgcagcgacg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa
     4921 tctaccttaa ctaccttctg ggaatatctc gcgcaacaag accctgaatt acccgctatc
     4981 cagacgctcc cgatggatgg ttttcaccat tacaatagct ggctggatgc gcatcaattg
     5041 cgccccttca aaggcgcacc agagacattt gacgttgcga aactggcgga aaatctgtgc
     5101 caggtagtgg aaggggattg tacgtggccg cagtatgatc gacaaaagca tgatcctgtt
     5161 gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gttgttgctg
     5221 gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt tatcaacgcg
     5281 ccagctactg cgttgcggga acgactggta gggcgcaagc tggcgggagg ggtatcgctg
     5341 gcagacgcag aagcttttta tgaccgtacc gacgggccga atgttcgccg ggtgctggaa
     5401 gagagtctgc ctgcaaattt gaccttaatg atgaccgcta cgggtgaata tcgtctggtg
     5461 gattaacgtt cctgattaga ccatcttcca gtcgcaccac acgagagaaa tgacgccggg
     5521 taaatgcggc gtcatgactg attgctacga cgctcgtgcc tcgctggcgg cacttctcca
     5581 gccagctttc aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc tcgtccagca
     5641 gcaatagtgg cggagagagt gcttccaggc aggcgacggc gaccatccgt cgctgcgcac
     5701 tatgcagatc caacgggtgc gcgttggcag tgtctgctaa ctgacaacat tgcagggcag
     5761 catgggtacg ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga
     5821 tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc ttcctgaaac agtacgccaa
     5881 cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc ctgttgttgc agcataaccg
     5941 taccggctgt gggcgtaaga agacctgcca ttactcgtaa cagcgtcgat tttcccgcgc
     6001 cattgtcgcc ggtcagcgcc aaccattcac cttgtttgag ttgcagagaa atgtcgcaaa
     6061 gacaatccgt tgccgcgcca ggccagcgat aagatatctt atttaacgtt aacatagtgg
     6121 ctggagcgct ccgttttgca attgccacgc ttgctggcag tagttcagga aaggtgtgcg
     6181 atgacgttca aaaagaataa tcagcgaacc acgttcaaac gcccagtgtt gtaagcgctg
     6241 caacagcatt tcgctggcct gcggagtcag gcggctaaag gcttcatcaa gaattaagag
     6301 ctttggctgc atcgcgatgg cgcaggcgat gaccacgcgc tgtgtctctc caccggagag
     6361 tgttgccggg tgacgatggc gcaacggctg gcactcggtc agagtcaggg cggcatcaat
     6421 ccgcgccata atctctgctt cagcaagaca cagattttct ggcccaaagg caacttcttc
     6481 ttccacgcta aaggtacagc cggagagctg caaatagggc gattgctgga cgagctggat
     6541 ggtggcggat tgttcattaa gcgggagatg cccaatgggc gtaccgagga gcgtccccgt
     6601 gcctgttatc tctcctggca gaaagtccgg ataccagccc gccatcagct gtgccagcgt
     6661 acttttgcca ctgccgttgt caccaaagat ggcgaccatc cccggtttga catagtgaaa
     6721 gtcataacag aacggaggat gcgttgagtg tgtcgggcaa taacggaact gttctaacgt
     6781 aaccatatcc acgctccgaa ttctgtcagc atcagcaaga tcatggtgta acgcgctacg
     6841 cgttgtaggg tgctatcggc tggtgcccac aacgttgttc gtcggttgtg gatccgaaat
     6901 gcccgcatat ccagcgccgc accacggaca gccagatcgt taagcgcatt gtgtgttagc
     6961 ggaataatta acgcgggcat cgcacgtaaa cgctgatacc agccctcatc cagcggcacg
     7021 ccgcgggcac gctgcgcttc atgaataatc gctaattgcc gttttaactg ctcaacgacc
     7081 aataacggcc cggcaaacag ataagcgacg cctggcggca ggcgagaagc aaacagagcg
     7141 cgaataaaac attgcacggg gacgtactgc atccacaact gtgaggttga aacaatcgcc
     7201 agaatccgca accagagcgt aatagcatgc gaccaccgtt ctggcgagcg tggcgtgccg
     7261 cttagccatt ctggtagcca gccaccgtgt accagccaca gcccggctcc cagcgagaac
     7321 atcagccaga cgacatattt tgcccgccgc cgcgtggcct ttagcgcaat aaggcagaaa
     7381 aaggtcgccg cgctatagat cggcaaaatg gtctgcgcgg gcaggataag cgtggtgcag
     7441 gccaccaatg cccacagtgt taatgaggta aatggatgca tttagcgcac ggcagccatt
     7501 gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca gtaaaagcca gacaatcacc
     7561 gctgtaagaa ttttatcgac caggttcgcg cctatcaccg taatcgccac cgactccacc
     7621 aggttttgcc ccatcgagtg catccaggca acgaacaaat cggctccgct accggtgaca
     7681 ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg ttaccgcaag cgtaataatc
     7741 acgccgctga caacgacttt tggcagggtg cgaaaccaac ccgctcgcgc caggcaaccg
     7801 gcgaccaggc cgatgaccat cgcgacgggg gcaaatgcgg cggcgatcgg gtcggtcagc
     7861 aatccccaga gtaaattggt cagtaagccg gttagcatcc cgatcaccgg accaagcagt
     7921 accgcgctaa taagggtgcc gatggagtcg agaaatatag gtaatttgac catgctggcc
     7981 agttgcccgc cgatcatatt gatggcgata gatataacga tcaacaccag tgcctgactg
     8041 gaaaagtggc tacgcgccat aagaaaacct ctctgaaagt agttatttaa cgggggaaat
     8101 atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg ctaaacgcca gttcctcaag
     8161 gctaccgatc actagttcaa gcgtggtccg cacggtacag ccgggcatca tatgaccgcc
     8221 aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga tgctcaccac tctgttccag
     8281 cgtgccgttt agtgagatca cttcgaatgt accgttcagg agcgtggtgc cttcttgccc
     8341 ggcatagcgc aaggcgacgt cagtcaggct accggtacag cctgcaatcc aggcggcatg
     8401 aagttgctgc tgctgtgcaa aagcgtgtag ctgggaaaaa acttcctgcc cggggagcag
     8461 gcgcagtgcg taaaagcgtg cggaggaaga atatgggtag ggcaatgtca tcattggagt
     8521 actccgaaac tcggacgtat tgcggataat atcgacgctg catcagtcat attctgatat
     8581 aagtcagggt ttgtgacggc acaacgaaaa gattttgcta cgcttattcg tgattctgca
     8641 aagtggaata aattccacag gggcggggct gaaaacgtga cgtaagtcag atatttgttt
     8701 tctttgactg gacaatcatt ccttttattc cacgtttcgc ttatcctagc tgaagcgttt
     8761 cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag gtaaggtttc
     8821 cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa ccttgcagga
     8881 gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt aatgtggttc
     8941 gtgctttgta tgaatcagga cgccgtgcgg aaattaccgt ggtggcaatc aacgaactgg
     9001 cggatgctgc gggcatggcg catttgttga aatatgacac cagtcatggc cgttttgcat
     9061 gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc cgcgtattgc
     9121 atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta gtcctcgact
     9181 gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc ggggccaaaa
     9241 aagtgctctt ttcacatcct ggcagtaacg atctcgacgc gaccgttgtt tacggcgtca
     9301 atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt accacgaatt
     9361 gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc ggcactgtga
     9421 ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat cctgacctgc
     9481 gtcgcacccg ggcagccagc cagtcgatca ttccggtcga tactaaactg gccgccggta
     9541 tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta cgtgtgccaa
     9601 ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta aaagccaatg
     9661 aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata gttgactata
     9721 cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc attgtcgatg
     9781 gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg tgcgataacg
     9841 aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact gttgctttca
     9901 ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt caccatgtct
     9961 gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg tgcggatctg
    10021 aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc ttctctgccg
    10081 accatcgaac tggccctgaa acaaggcgca aaagtgatgg taacttccca cctgggtcgt
    10141 cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa ctacctgaaa
    10201 gacaaactgt ctaacccggt tcgcctggtt aaagattacc tcgacggcgt tgacgttgct
    10261 gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa gaaagacgac
    10321 gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga cgcattcggt
    10381 actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga cgttgcgtgc
    10441 gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa agaacctgct
    10501 cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac cgttctggac
    10561 tccttgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa cacctttatc
    10621 gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt tgacgaagct
    10681 aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgcgt agcaaccgag
    10741 ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc tgacgagcag
    10801 atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa gaatgcgaaa
    10861 accattctgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg caaaggtact
    10921 gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct ccatcgctgg cggcggcgac
    10981 actctggcag caattgacct gttcggcatt gctgacaaaa tctcctacat ctccactggc
    11041 ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc gatgctcgaa
    11101 gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt tcagcgcgct
    11161 tttagagcac gcattttttc atactctaaa taattcgagt tgcaggaagg cgacaagaga
    11221 gtgaatcccc aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac
    11281 atgcaacttg aagtatgatg agtataaggc ccgacgatac aggacaagag acatgtctaa
    11341 gatttttgat ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga aagttttcca
    11401 ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta ctgactccat
    11461 caacgccgta ctggaaactg ctgctaaagt taaagcgccg gttatcgttc agttctccaa
    11521 cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc agggtgctgc
    11581 tatcctgggc gcgatctctg gtgcgcatca cgttcaccag atggctgaac attatggtgt
    11641 tccggttatc ctgcacactg accactgcgc gaagaaactg ctgccgtgga tcgacggtct
    11701 gctggacgcg ggtgaaaaac acttcgcagc taccggcaag ccgctgttct cttctcacat
    11761 gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa atctgctcta aatacctgga
    11821 gcgtatgtcc aaaatcggca tgactctgga aatcgaactg ggttgcaccg gtggtgaaga
    11881 agacggcgtg gacaacagcc acatggacgc ttctgcactg tacacccagc cggaagacgt
    11941 tgattacgca tacaccgaac tgagcaaaat cagcccgcgt ttcaccatcg cagcgtcctt
    12001 cggtaacgta cacggtgttt acaagccggg taacgtggtt ctgactccga ccatcctgcg
    12061 tgattctcag gaatatgttt ccaagaaaca caacctgccg cacaacagcc tgaacttcgt
    12121 attccacggt ggttccggtt ctactgctca ggaaatcaaa gactccgtaa gctacggcgt
    12181 agtaaaaatg aacatcgata ccgataccca atgggcaacc tgggaaggcg ttctgaacta
    12241 ctacaaagcg aacgaagctt atctgcaggg tcagctgggt aacccgaaag gcgaagatca
    12301 gccgaacaag aaatactacg atccgcgcgt atggctgcgt gccggtcaga cttcgatgat
    12361 tgctcgtctg gaaaaagcat tccaggaact gaacgcgatc gacgttctgt aagatattcc
    12421 tttctgctta tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc aaaattgcct
    12481 gatgcgctac gcttatcagg cctacacgta tcctgcaata tattgaattt gcaggttttt
    12541 gtaggccgga taaggcgttc acgccgcatc cggcatgaca aagcgcacat tgatatctgt
    12601 ctgagccccg ctctgcgggg attttttcgc caaaagcaag gtgattcaga tgagaaatct
    12661 gtgatctatt tggcaaaatt atgctttatt gtttaccctt gtcagactgc ccgtcataag
    12721 gcggcggagt gtatttctcc attttgagtc agttgaaaag gaatattgaa tggaagattt
    12781 gaatgttgtc gatagcataa acggcgcggg aagctggctg gtagctaacc aggcgctgct
    12841 gctaagttat gcagtaaaca tcgtggcggc actcgcgatc atcatcgttg gtttgattat
    12901 cgcgcggatg atttccaacg cggtgaatcg cctgatgatc tcccgtaaaa tcgatgccac
    12961 tgttgctgat tttctttctg cattagtccg ttacggtatt atcgccttta ctctaatcgc
    13021 tgcactggga cgcgtgggtg tacaaaccgc gtccgtcatt gctgtactcg gtgccgcagg
    13081 cttagctgtt ggtctggctt tgcaggggtc actgtctaac ctggccgctg gcgtgttact
    13141 tgtcatgttc cgcccgttcc gtgccggaga atatgttgac ctcggcggcg tagccggtac
    13201 tgtgctgagt gtgcagattt tctccaccac catgcgtact gcagacggta aaattatcgt
    13261 tattccgaac ggtaaaatta ttgccggaaa tattattaac ttctcccgcg agccagttcg
    13321 ccgtaacgaa tttattattg gcgtggcgta tgattccgat atcgatcagg ttaagcagat
    13381 cctgaccaat attatccagt ctgaagatcg cattttgaaa gatcgcgaaa tgactgtgcg
    13441 cctgaacgaa cttggtgcat cgtcgattaa tttcgtggtc cgcgtctgga gcaacagcgg
    13501 cgatctgcaa aacgtgtact gggatgtgct ggagcgtatt aaacgtgaat ttgatgccgc
    13561 cggtatcagc ttcccgtacc cgcaaatgga tgtgaacttt aagcgggtga aagaagacaa
    13621 agctgcgtaa tcaacgctga ggccagataa tactctctgg cctctctctt attagttttt
    13681 ctgattgcca attaatatta tcaatttccg ctaataacaa tcccgcgata tagtctctgc
    13741 atcagatact taattcggaa tatccaacgt gttttcttat tactttcaag gtcttgcgct
    13801 tggcgcagct atgatcctac cgctcggtcc acaaaatgct tttgtgatga atcagggcat
    13861 tcgtcgtcag taccacatta tgattgcctt actttgtgct atcagcgatt tggtcctgat
    13921 ttgcgccggg atttttggtg gcagcgcgtt attgatgcag tcgccgtggt tgctggcgct
    13981 ggtcacctgg ggcggcgtag ccttcttgct gtggtatggt tttggcgctt ttaaaaccgc
    14041 aatgagcagt aatattgagt tagccagtgc cgaagtcctg aagcaaggca gatggaaaat
    14101 tatcgccacc atgttggcag tgacctggct gaatccgcat gtttacctgg atacttttgt
    14161 tgtactgggc agccttggcg ggcagcttga cgtcgaacca aaacgttggt ttgcgctcgg
    14221 gacaattagc gcctctttcc tgtggttctt tggtctggct attctcgcag cctggctggc
    14281 accgcgcctg cgcacggcaa aatcacagcg cattatcaat ctggtagtgg gatgtgtcat
    14341 gtggtttata gccttgcagc tggcgagaga cggtattgct catgcacaag ccttgttcag
    14401 ttaggcgcta tctgatggaa aaataaaaca gaggcgctaa gcttgcctcc agaggtcctg
    14461 aattttgggc aagtcaatta acacggagag actaacgtga agttcaaagt tatcgccctg
    14521 gcggcattaa tgggtattag cgggatggca gcgcaggcta acgaattgcc ggatggaccg
    14581 catattgtca cctccggtac ggcaagcgtg gatgcggtgc cagacattgc cactcttgcg
    14641 attgaagtta acgtggccgc gaaggatgcc gctactgcca agaaacaggc agatgagcgc
    14701 gtcgcacaat acatttcctt ccttgaactc aatcagatcg cgaaaaaaga tatcagctca
    14761 gcgaacttac gcacccagcc agattatgat tatcaggatg gtaaaagtat cctgaaaggc
    14821 taccgcgctg tgagaacggt ggaagtcacg ctccgtcagt tagacaaact gaattccttg
    14881 ctggatggcg cgctgaaggc gggtcttaac gaaattcgtt ctgtgtcgct gggcgtggcg
    14941 cagccggatg cctataaaga caaagcgcgt aaggcagcga ttgataacgc gattcatcag
    15001 gcgcaggaac tggcgaacgg ctttcatcgt aaactggggc cggtatatag cgtgcgctac
    15061 catgtttcca actatcagcc cagcccaatg gtgcggatga tgaaagccga tgccgcgccg
    15121 gtgtccgccc aggaaactta cgagcaggcc gctattcagt ttgatgatca ggtcgatgtg
    15181 gtcttccagt tagaacctgt ggatcaacaa cccgctaaaa cacctgcagc acaataatac
    15241 ttgtagcccg acgtattcgt cgggctaatg cctttacccg atattgcctc ctttttctgt
    15301 aattttctgt aaaaccatga cgtaatcacc tatctttgct cgcggtttca tcaaggatag
    15361 gctgactgaa acggttaagc ggacaaggat gcgggggagg gggatggaca tttttatttc
    15421 gaaaaagatg cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga
    15481 aaaaatccat atgacggctt cgccatttgg taaaagcatt gccgcgctgg aagaacaaat
    15541 tggctatacg ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggacagga
    15601 actgtatcaa aaactgttcc cggtttatca acggctttct gcgattgata atgaaatcca
    15661 taactcagga cgtcgttcac gagaaattgt gattggcata gataatactt atccgacaat
    15721 tattttcgat cagttgatta gtctcggaga taagtatgaa ggcgtgaccg cccagccagt
    15781 tgagttcagc gaaaacggcg ttatcgataa tctttttgac cgccagttag attttattat
    15841 ctccccacag catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaact
    15901 accaccgctg cgattaggct ttctcgtttc tcgccgttat gaagagcgtc aagagcaaga
    15961 gctgcttcag gagctgccct ggctacaaat gcgctttcag aaccgggcca acttcgaagc
    16021 aatgattgat gcgaatatgc gaccttgtgg gatcaatcca accattattt atcgtcccta
    16081 cagttttatg gccaaaatca gcgccgtgga gcgcggacat tttctgacgg tgatcccgca
    16141 ttttgcctgg cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc
    16201 gatgtatatg caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatgct
    16261 tcagcatatt gctgaagatc gtgacgggac aaaccattaa cccagcatcg agccggttgc
    16321 aattaaatta cggtgtaagt cgaagacgtg gctaagatcg tggtgaatat gtccgccggg
    16381 cgcattttcc agatagcgat gcagataatc ccgatacata ggatgtgcac aattatcaat
    16441 gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga tcggcgatcc cttgttcggt
    16501 gatgatcact ttgacgctgt gttcgctgtg atcaacatgg ctgcacattg gcacgacggt
    16561 tgagatcttg ccttctttag caatcgacgg agccataaag atcgacagat acgcgttgcg
    16621 ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg accccggcta cgtgcgttga
    16681 gttggcatgc ccgtaaatat caaactccag gccgacgttc agagcgatga cgcccagacg
    16741 acggatgatt tccgggttat tggaaatctc ctgcggacgc aacacaatgc ggctggcaaa
    16801 gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc gagattgtca ggctggaggc
    16861 gctggccccg ctgattttgc cggtttccag taaatgcacc accgattcct gtagcacttc
    16921 cgaatacatc ataaacggag gaatttccgg gttttccccc agacgcgcca ttaccgcatt
    16981 attgatattg cccacgccac tttgcagcgg cagaaattcc ggcggaatac gcccatgcgc
    17041 catttcctgc aataagaacg tgaccacgtt atcggcaatc tgctggcaca tgggattttg
    17101 cttatccagc atattaccgg cgtcgggcaa gttggtttcc acgacggcga caatcttttt
    17161 cggatcgatt tgcacatagc gggtaccgac gcgatccatt gcatggaaga tcgacacgct
    17221 attgcgccgc ggtggcgcgc cagggatcac aatatccgcc agttctgcaa cgcgcggatc
    17281 gtgatagtta ttgagttcaa tgatcacttt cttcgctcgc agcagccagg tcggcgcatt
    17341 accgatcccg ctggttaacc agactcgacc atccggtgcc agtgccgatg cttcaatgac
    17401 ggcaacatca atgtcgccga agaaaccgta attgaccatt tgcgccactt cgctcaaatg
    17461 caggtcaacg aaactcaccg cgccctgatt gatcttttta cgtaaaccgg acgatgtttg
    17521 atatggcgca cgccaggaaa cagcatcggc gtcagaaagt acatcgtcag cggcggcgct
    17581 gattgacgcc cccgtcagaa ggcgaatttg atacggcttt ttggcctcat gctgttcgtt
    17641 agctctgcgg gcaatcgcgg tgggtagggc tttcggcgaa cccgccgggg taaagccgct
    17701 aaatgccacc atgtcgttat gctggataat ttctgccgct tcattggcgg tcatccttgt
    17761 ccactgagtt tccattttta ctccttcgtt cgcagggatt aatgaccaac gaaattaggt
    17821 ttacgttttt cgaggaaagc gttcatccct tcctggtaat cttcgctgtc ataaaccgcg
    17881 cggcgcatcc cctgaatacg ttcaaattca tcggagttca tggtgtgcgc ttcgcccagt
    17941 acacgcagct cttctttgat aacagcaatg gctaacggcg ctttctcaga gatgtggtgc
    18001 gccatttgta aggtgaaatc ttccagttct tccacttcca caacatggtt gaggatgccg
    18061 acagccaacg cgcgctgggc ggtgattggc gaagcggtaa aaatcagttc tttgacaatg
    18121 tggaagcccg cgtcgcgggt caggttgtga atgccgacca ggttatacgg gacgccgagg
    18181 tttacaggcg tcattgagaa ggttgaggta ctggcggcga taatcagatc ggaactcatg
    18241 atcatttcaa atgcgccacc ccaaacacta ccttccacca tcgaaatgat cggtttcggg
    18301 aatttttgga tcatgcgggt gatttgacgc aatggatcat cataggagag cggatcgcga
    18361 ccgccagacg gcagttcgtg aatatcgtgg cctgcggaga agactttgga tccactcggc
    18421 gcgcgcaaaa tgatacagcg aatttccggc cggttgagat cgcttaatgc ctgcataaga
    18481 tcatcaataa agactttact taaggcatta agtttgcggc catagttaaa ctcaatgacc
    18541 gccactttgt tgatagtgac aacgttaaca tactgataag acataaaaat tcctttaatc
    18601 aaaatattgc gtctggataa attcactgag ctgccgcagg ccggtgcgcg gtgagagcgt
    18661 attgtttttg accgctaaaa gcgtctggcg gtaatagcga tcgaaatctt cattcgcgaa
    18721 caggtgattc agtacttctt cttcggtctg cttacgcagc cattccaccg attgttgttg
    18781 ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt ttgaagtcga tgatggcgtg
    18841 ccagatctca tcgattccac gtttttccag tgcgctacaa gtcagaaccc gtggctgcca
    18901 ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg tacatatgcc gggcaatggc
    18961 gacattggta tggttatcgc catcgtcttt gttgataacg agcagatcag ccacttccat
    19021 cagccctttt ttaatgccct gcagatcatc gccgccaccg gcaatttgca acgagataaa
    19081 acagtccacc atgcgggcga cttccgtttc cgactgcccg acgccaaccg tttcgacaat
    19141 cactacgtca taacccgccg cttcgcataa cagcattaat tcccgcgctc gctgactggc
    19201 accgcccaga tgaccggagg atggtaccgg gcgaataaac gccgcttcgg cacgcgccag
    19261 gtcattcatg cgggttttat ccccgagaat gctaccgcca gtgaccgggc tgctgggatc
    19321 gaccgcaata accgcgacct ttaatccctc acgaatcaac aacatgccaa aggcttcaag
    19381 aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc agtcgcaggg cgttaccgca
    19441 gtacggcata atggcatcaa gcagctgcgt acttagtgcc tgatgacgcg ggtgacggct
    19501 ttccaccagc gtcatggcct gggcgagtgt ggcacgctca ccctgacgta agcggcgaat
    19561 actttctgcc agcgtggctt cattaatcat gatgctggct tatcagattc agtacgccgc
    19621 gcacgctgtc gagcataggt gtacctggac cataaatcgc cgccacgccg cgctcttgca
    19681 ggaaggcgta atcctgcggt ggaatgacgc caccggcgac tacgcagata tcttcgcgtc
    19741 cccatttttt cagcgcttcg accagttccg ggatcagcgt tttatgaccg gcagccagtg
    19801 aggatgcgcc cactacgtga acgtcgtttt ctacggccag gcgggcgatc tcttcaggtg
    19861 tagagaacat cgggcttaaa tctacgtcga aaccgagatc ggaataggcg ctggcgatca
    19921 ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc gatcagaatg cgcgggcgac
    19981 gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat ggcatcgaac tcggaggccg
    20041 atttctcaga ctgatgatag ctttgcgcaa tcacgccggt aacacactgg cttggcacca
    20101 gataacggtc gaaagcgact tccagcgcat cggaaatttc acccagggtg gcgcgaacgc
    20161 gagcggcatt aacagcggca gccagcaggt tttcgttatg ctgtgcggcg tgagtcaggg
    20221 cgttcaacgc ggcggttacg gcggcatcat cacgggtggc gcgaatgcgt tccagcgaag
    20281 caatttgctc gttacgcacc atcacgttgt cgatctcaag tacatcggtt tcgtcttcgt
    20341 gatccagttt gtacttgttg acaccaacga tgacacgctt gccctggtcg atcagcgact
    20401 gttcgcgcgc tgaggcctct tcgatcattc gttttggcag acctgcttcg atcgctttcg
    20461 ccatgccacc ggcttcgtcg atctgttgga taatagctct ggcttgtttg acgatttgat
    20521 cggtcagcga ctcaatgtaa taggatccgg ccagtggatc gacggtgcgg cagagttctg
    20581 attcttcctg gatgatgatc tgggtgttgc gggcaatgcg tgctgagaaa tcggtaggca
    20641 aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg agtaccgccc agcgtcgcag
    20701 ccagcgcttc aatggtggtg cggataacgt tgttatacgg atcctgttca gtcaggctcc
    20761 agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt cggatcctgt gcgccaaatc
    20821 cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa catggcgacg ttcataaaca
    20881 gatccatgcc gataccgaag aagaacgaca ggcgaggagc gaagtcatca attttcagtc
    20941 cggcagagat tgctgctttg atgtactcaa tcccatcagc gagcgtaaat gctacctgct
    21001 gcacgcagtt ggcacccgct tcacccatgt ggtaaccgct gatactgatg gtattaaatc
    21061 gcggcatgtt gccggaacac caggcgatga tgtcggcgat aatgcgcatt gacggttttg
    21121 gcgggtaaat ataggtgttg cggcagaggt actctttgag gatatcgttc tgaatggtgc
    21181 cggtcagttt atcaggtgta acaccttgct cttctgcggc gacgatataa aacgccagta
    21241 ctggtagcac tgcgccattc atggtcatcg aaaccgacat tttatccagc gggatctggt
    21301 cgaacaggac tttcatatct tccacggtgt cgatagcgac gcccgctttg ccgacgtcac
    21361 ccgccacgcg tgggttatcg gagtcgtagc cacggtgggt ggcaaggtca aacgcaacgg
    21421 aaagaccttt ttgcccggcg gccaggttac ggcgataaaa agcgttggac tcttttgctg
    21481 ttgaaaaacc agcatactga cggatggtcc acggttgggc ggtatacata gtggcacgcg
    21541 ggccacgaac gtagggcggc aaaccaggaa gggtacctgt cacctccaga ttatcgagat
    21601 cggcttcggt atacagcggc ttgatggcga tcccttccgc ggtttgctga accagcgagt
    21661 cgacagtttt ctcccaacgg ctcaattcct tgttggcaag ctgttgccac tcctgctcgt
    21721 tagacattat ttgctccgta aaacgcaatc aaaatgaggg tatttgcgct tacggtgagc
    21781 tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca gacgtcgttt tttgccgcct
    21841 gagatttgtc tattttgtga tgtgattcaa ggaatacaaa aagaagtgag gacaacgcct
    21901 gatatgtgct atcaggcatt atttgatgga ttaatcctga cgaaggactt tgtgaccata
    21961 atcgagtaac gcatcagtga ctttacgcat catgcggctt tcaggagcaa agcggtgcca
    22021 gtagagcatc cgtcgttgaa atagcccagg cgttaagtca atcagttcac cgctggccag
    22081 ctctttctcg atttgcaggt gcgggatcat acagcaggtg gtgccctggc gagcaagttg
    22141 tacgaacgct tctgaagaat taacgatatg gcagggcacg ctgcctggag gcagatcgaa
    22201 gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg tcaaacgcga ccactggcgc
    22261 tttcagtaat gccgaacgcg ttacgccgtt agggaaatat ttttcggcaa agggttttga
    22321 gctgacgaac agatagtcga gcgcaccaag tttatcgaca agacaactcg gcagcgcctg
    22381 atgttgaata ctcacagcgc cgaccacttc gccgcggcgc agacgttcct gagtgcgggt
    22441 ttcatcttct acctgcaagt tgaggcggat aggcgaatca gccaacacag gagccagtgc
    22501 aggaagcaac cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca gcagcggagt
    22561 cgaaccggtt tgttcatcgc ccagccactc ttcttccagc aactccacct ggcgcagcag
    22621 tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt acggtacgca ccaacagcgg
    22681 ctgcccgaac atattttcca gttgcttaat gcgctgtgag acggctgatt gtgtaatgca
    22741 cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc accgcatcca gtgcctgtaa
    22801 tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc tggtaaatgc tttttcctgc
    22861 actatgacac aattttgtgt caggttgcat ataccgaagc cgtcggcggg taacatttgt
    22921 attcgtggtt aaacgcgcag cttaaaagat cagggatttg cgctaatgca tttcgctcag
    22981 cgagttcgcg ccctggttgt gttgaatggc gtggcgttat tgcctctgtt cgcaccgtgg
    23041 agcggcatac ccagaccgtt gtatgcttta tttgcggggc gaaaggggat gcccgccatt
    23101 gcgcgatatt ttatggatga gttaaccacg cggcttgcca acggggtctg aatcgctttt
    23161 tttgtatata atgcgtgtga aatttcatac cacaggcgaa acgatcatga cgcaggatga
    23221 attgaaaaaa gcagtaggat gggcggcact tcagtatgtt cagcccggca ccattgttgg
    23281 tgtaggtaca ggttccaccg ccgcacactt tattgacgcg ctcggtacaa tgaaaggcca
    23341 gattgaaggg gccgtttcca gttcagatgc ttccactgaa aaactgaaaa gcctcggcat
    23401 tcacgttttt gatctcaacg aagtcgacag ccttggcatc tacgttgatg gcgcagatga
    23461 aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg gcgctgaccc gtgaaaaaat
    23521 cattgcttcg gttgcagaaa aatttatctg tattgcagac gcttccaagc aggttgatat
    23581 tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg gcacgtagtg cagtggcgcg
    23641 tcagctggtg aaactgggcg gtcgtccgga ataccgtcag ggcgtggtga ccgataatgg
    23701 caacgtgatc ctcgacgtcc acggcatgga aatccttgac ccgatagcga tggaaaacgc
    23761 cataaatgcg attcctggcg tggtgactgt tggcttgttt gctaaccgtg gcgcggacgt
    23821 tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg aaatgatctg acgggggaac
    23881 ctcccccgtt aaaaaaattc tcttcattaa atttggtgac atgtgtcacg cttttaccag
    23941 gcaattgtcg attgctctaa ataaatcctc taaaccagca tattcatcca agaattacct
    24001 ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat attgccgcaa tattattttt
    24061 tgatatgttg aaaggcggat gcaaatccgc acacaacatt tcaaaagaca ggattgggta
    24121 aatggcaaag gtatcgctgg agaaagacaa gattaagttt ctgctggtag aaggcgtgca
    24181 ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc aacatcgaat ttcacaaagg
    24241 cgcgctggat gatgaacaat taaaagaatc catccgcgat gcccacttca tcggcctgcg
    24301 atcccgtacc catctgactg aagacgtgat caacgccgca gaaaaactgg tcgctattgg
    24361 ctgtttctgt atcggaacaa accaggttga tctggatgcg gcggcaaagc gcgggatccc
    24421 ggtatttaac gcaccgttct caaatacgcg ctctgttgcg gagctggtga ttggcgaact
    24481 gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa gcgcaccgtg gcgtgtggaa
    24541 caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag ctgggtatca tcggctacgg
    24601 tcatattggt acgcaattgg gcattctggc tgaatcgctg ggaatgtatg tttactttta
    24661 tgatattgaa aacaaactgc cgctgggcaa cgccactcag gtacagcatc tttctgacct
    24721 gctgaatatg agcgatgtgg tgagtctgca tgtaccagag aatccgtcca ccaaaaatat
    24781 gatgggcgcg aaagagattt cgctaatgaa gcccggctcg ctgctgatta atgcctcgcg
    24841 cggtactgtg gtggatattc cggcgctgtg tgatgcgctg gcgagcaaac atctggcggg
    24901 ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat agcgatccat ttacctctcc
    24961 actgtgtgaa ttcgacaacg tccttctgac gccacacatt ggcggttcga ctcaggaagc
    25021 gcaggagaat atcggcctgg aagttgcggg taaattgatc aagtattctg acaatggctc
    25081 aacgctctct gcggtgaact tcccggaagt ctcgctgcca ctgcatggtg gtcgtcgtct
    25141 gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg ctgaacaaaa tcttcgccga
    25201 gcagggcgtc aacatcgccg cgcaatatct acaaacttcc gcccagatgg gttatgtagt
    25261 tattgatatt gaagctgacg aagacgttgc cgaaaaagcg ctgcaggcaa tgaaagctat
    25321 tccgggtacc attcgcgccc gtctgctgta ctaattcccc ttctctgaaa attaacgggc
    25381 aggtcactga cttgcccgtt tttttatccc ttctccacac cgttgccgcg ttgcgcattc
    25441 tactttgcga gtctgctcca gaaaataaat caaagtcaga tcaatgcgtt gtgtttattt
    25501 aatggggatt tacgatggcg ggacagcatg gggctgtaac ggtaaagccc tcaccgaagc
    25561 gagggcttaa aggagaaggg ttatgatgcg acttgtcatc atactgattg tactgttact
    25621 gataagtttc ggagcttatt aacagtcagt ctcaggggag gagtcatcct cccttaccct
    25681 tactcactaa attaggtcaa agaatcaacg atgtcaatca gggcgatgcg gggtgcatcg
    25741 ccctgaaccg aactttttga tagccacttc atcgcccacg tttactgccg taatagatcc
    25801 tttttcccaa aaattatctc tgttttgcat ggggtttttc gagcacgctc gaagaaaaga
    25861 tttaaaggta gatcaatgcg ttgtggttat ttaaagagag cttacgatag ctgggcagca
    25921 tggggctgta acggtaaagc cctcaccgaa gcgagggctt gaaggagaag ggttatgatg
    25981 cgacttgcca tcatactgat tgtactgtta ctcataagtt tcccagctta ttaacagtca
    26041 gtcacagggg aggagcaatc ctcccttacc cttactcact aaattaggtt aaagaatcaa
    26101 cgatgtcaat cagggcgatg caggtgtatc gcccttacca ctcccagact ttcgacggtg
    26161 taaccaccgc aggaagaggg atatcccact cttcaacggg gagtttttcc accaactgac
    26221 aatcatgcgc ataacccacc ggttgcgttt tatagtgctg ccagttttgt aaggtccgat
    26281 cataaaaacc accgcccatt cccaggcgct gaccgtactc atcaaaggcg accagaggtg
    26341 tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg cacatccagt tttggctcat
    26401 ggatcttcaa cctgttcatc accagttcgc tttgcggatg gtaattcagg aacagcaaat
    26461 taccggcact aaagggatgc aaaactggaa gatatacgcg cttaccggcg cgccagagtt
    26521 gttctattag cggctgggtg tcgagttcac catcaaaaga gaggaataca gcgaccgtat
    26581 gcgccatcac caccggggga taagtcatca tccgggtagc ggcttgttga cccatttcct
    26641 gctgttgttc cggcgttaac gcacgacgac gttgccgaat cattttgcgg atttcttgtc
    26701 gggataatgt cagtgggagt tctggtagtt gcgtcatggc tggtgccaga taagaaggga
    26761 atctccgaga tgccgccgca ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc
    26821 gtcgcagttt taaggcttct cggacggacc gagcatgctc accaaccgcg gagcgccaca
    26881 ttcttgtggt atgaaatatc ggctcagggg actggcccgc ttgcgaacat ctcagagaaa
    26941 ttttgtcttc acggttactc taccacagta aaccgaaaag tgtcattcaa agttttggtt
    27001 agttttttcg gtgatgcgac cttgttcaag taacgcttgt tctatggtct gctgcagcat
    27061 ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc ttcgcttttt cttgcgctaa
    27121 ctcatagctg atattcaatg cggcaatgaa gaccaactgt tcagtatttg tgactctagt
    27181 gcgttctttc agatcttgca accgttggtt cagatcgtcc gctgcctgat tcaacgcatc
    27241 cctttggtca ggcgggcagt tcacacgcag tgaacggcca aaaatttgga tatcgacggg
    27301 ttgtgcagac atgccacctt cctgctgatt gactgcgctg ccttcgtctt tagacccatg
    27361 gtctgcgaag gggcgacact atagctaccc tgatgagaag agacaagccc ttttctggtc
    27421 caccagggcc caaagtggta gcatatcatg aatattcctc cctttgacga cgaatgctta
    27481 tgtctataca gaacgaaatg cctggttaca acgaaatgaa ccagtatctg aaccaacaag
    27541 ggacgggtct gacccctgct gagatgcatg gtttaatcag tgggatgata tgtggcggta
    27601 acgatgacag ctcatggcta ccgctacttc acgacctgac gaacgaaggc atggctttcg
    27661 gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg
    27721 atgacggctt cctttttcag ctttatctgc ctgatggcga tgatgtcagc gttttcgatc
    27781 gggctgatgc attggcaggt tgggtcaatc acttcctgct tggtcttggc gttacgcaac
    27841 cgaagctgga taaagtgacc ggcgaaaccg gtgaagctat cgacgatctg cgtaacattg
    27901 cgcaactggg ttacgacgaa gacgaagatc aggaagagct tgaaatgtcg cttgaagaga
    27961 tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga cacctttact catccgcaac
    28021 cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa acaaaaaacg taaggagagt
    28081 gttatgagtg agatatcccg gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa
    28141 atgcaacccg gcagcgccgc gctgattttt gctgcaccag aagtaacacg tagcgccgac
    28201 agcgaatacc cctatcgtca gaacagtgac ttctggtact tcaccggctt taacgaaccg
    28261 gaagcggtgc tggtgctgat taaaagcgat gacactcata accacagcgt tctgtttaac
    28321 cgcgttcgcg acctgacggc ggagatctgg tttggccgtc gcttaggcca ggatgccgcg
    28381 ccagagaaac tgggcgttga ccgcgcactg gcattcagcg aaatcaatca gcaactttat
    28441 caactactta acggcctgga tgtggtttac catgcccagg gcgaatatgc atatgctgat
    28501 gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt cgcggcaaaa tctcaccgca
    28561 ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcgcca
    28621 gaagagattg ccgtactccg ccgcgcggga gaaatcaccg ccatggcaca tacacgggcg
    28681 atggaaaaat gccgtccggg aatgttcgag taccatctgg aaggcgaaat tcaccacgaa
    28741 tttaaccgcc acggcgcgcg ctatccgtcc tataacacca ttgtcggcag cggtgaaaac
    28801 ggctgcattc tgcactacac cgaaaacgag agtgaactgc gcgacggtga cctggtgttg
    28861 attgacgcgg gttgtgaata caaaggttac gcgggcgata ttacccgcac cttcccggtc
    28921 aacggcaaat tcacccaggc ccagcgtgaa atctacgaca ttgtgctgga gtctctcgaa
    28981 accagcctgc gcctgtatcg tccgggaact tccattctgg aagtcactgg tgaagtggtg
    29041 cgcatcatgg ttagcggcct ggtaaaactc ggcatcctga aaggtgatgt tgatgaactg
    29101 atcgctcaga acgcccatcg tcctttcttt atgcatggcc ttagccactg gttaggactg
    29161 gatgtccatg acgttggcgt ttatggtcag gatcgctcgc gcattctgga accggggatg
    29221 gtactgaccg tagagcctgg tctgtatatc gcgccggatg cggatgtgcc agaacaatat
    29281 cgcggtatcg gcattcgtat tgaagacgac attgtgatta ccgaaaccgg caacgaaaac
    29341 ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc agctgcgaga
    29401 aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta
    29461 tttcccggtt aagtcacggg gcgctgccgg tacatttgat tgaagcgacc gcgccagagt
    29521 cacatgctca tccgggcttt gatggacgag ctattgcgct agcggcgggg acctgtcagc
    29581 aactggcgcg catcggcgtc tggcaatctc tggctgattg cgcaaccgcc atcactaccg
    29641 tgcatgtcag cgatcgtggt catgctggat tcgtcaccct cgccgcagaa gattaccaac
    29701 tggcggcgct ggggcaggtt gtcgaattgc ataatgtcgg gcaacggctg tttgcattgc
    29761 tgcgaaaagc acctggcgta atgctgcatt gccccgatcg cgtagctaac gttgcccgta
    29821 ctcagagtca cgttgaagtg acgctggaga gcggcgagat gctgacgggc cgcgtgctga
    29881 tagccgctga tggcacccat tcagcgttag ccaccgcgtg cggcgttgat tggcagcagg
    29941 agccttacga acaactggcc gtgattgcca acgttgcgac ttccgttgcg cacgaaggcc
    30001 gcgcttttga acgcttcacg caacatggcc cgctggcgat gttgccgatg tctgacggac
    30061 gctgttcgct ggtctggtgt catccactgg aacggcgcga agaggtgctg tcgtggagcg
    30121 acgagaagtt ttgccgtgaa ctccagtcgg cctttggctg gcgacttgga aaaattaccc
    30181 acgccggtaa acgcagtgct tatccgctgg cgttaacccg cgccgccaga gctattaccc
    30241 atcgtaccgt gctggtgggc aatgcggcgc aaactctgca cccgattgcc gggcaagggt
    30301 ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac cctaactcag gcgcatgagc
    30361 gcggagaaga catcggtgat tacggcatat tgtgccgcta tcaacagcgt cgacagagcg
    30421 atcgcgaagc aaccatcggc gtcacggaca gcctggtaca tctttttgcc aaccgttggg
    30481 caccgctggt tgtcgggcgc aacatcggac tgatgacgat ggaattattc accccggcac
    30541 gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg ttgaaggcgg ttttaaggaa
    30601 taaacaatgc aaagtgttga tgtagccatt gttggtggcg gcatggtggg gctggcggtt
    30661 gcctgcggct tacaggggag cggcttacgc gttgccgtac tggagcagcg cgtaccggaa
    30721 cctctggcgg cggatgcacc accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa
    30781 aaattactca cccgtcttgg cgtctggcag gacattctct ctcgtagggc cagttgttat
    30841 cacggtatgg aagtgtggga caaagacagt tttggtcaca tttcgtttga cgatcaaagc
    30901 atgggctata gccatcttgg gcatatcgtt gaaaactcag tgattcacta cgcgctgtgg
    30961 aacaaagcgc agcagtcgtc agatatcact ctgttggccc ccgcagaatt acagcaggtc
    31021 gcctggggag aaaatgaaac tttcctgacg ctgaaagatg gcagcatgtt aacggcgcgt
    31081 ctggtgattg gcgcggacgg tgctaattcc tggttgcgca acaaagcaga tattccgctg
    31141 actttctggg attatcagca tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat
    31201 gatgcggtgg cgcggcaggt tttccatggc gaaggcattc tggccttttt accgcttagc
    31261 gatccgcatc tttgctcaat tgtctggtca ctgtcgccag aggaagcgca gcggatgcag
    31321 caggtaagtg aagatgaatt taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc
    31381 ttatgcaagg ttgagagcgc gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag
    31441 tttgccgcgc accgtctggt gctggtgggc gacgccgcgc ataccattca cccgctggcg
    31501 gggcagggag taaatctcgg ctttatggat gctgcagagc tgattgctga actgaaacgg
    31561 ttgcatcgtc aggggaaaga catcgggcag tacatttacc tgcgccggta tgagcgtagc
    31621 cgcaagcaca gtgcggcgct gatgctggct ggtatgcagg gattccgcga tctgttttcc
    31681 ggtaccaatc cggcgaaaaa actgctgcgt gatattggtt tgaaactggc cgacacgctt
    31741 cctggcgtta aaccacaact tatccgccag gcaatgggat taaacgattt gcctgaatgg
    31801 ctgcgttaaa aatttctctt ctgctgttta tttgataccc atcacacttt catctcccgg
    31861 ttttttcgcc gggagatttt cctcatttga aataaactaa tttcacctcc gttttcgcat
    31921 tatattttct aatgtcacga atttttgatt tagtgttttt tgacattttt tcagctctta
    31981 atatt
//
LOCUS       DAMZII010000047        31324 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZII010000047 DAMZII010000000
VERSION     DAMZII010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.047"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="POA77_002094"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="POA77_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6365534.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="POA77_002095"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="POA77_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI6365535.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="POA77_002096"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="POA77_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI6365536.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="POA77_002097"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="POA77_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI6365537.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="POA77_002098"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="POA77_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI6365538.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="POA77_002099"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="POA77_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI6365539.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="POA77_002100"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="POA77_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI6365540.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="POA77_002101"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="POA77_002101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI6365541.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="POA77_002102"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="POA77_002102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI6365542.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /locus_tag="POA77_002103"
     CDS             12889..13428
                     /locus_tag="POA77_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDI6365543.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="POA77_002104"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="POA77_002104"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDI6365544.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="POA77_002105"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="POA77_002105"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDI6365545.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="POA77_002106"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="POA77_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDI6365546.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="POA77_002107"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="POA77_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDI6365547.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="POA77_002108"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="POA77_002108"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI6365548.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEVDVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="POA77_002109"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="POA77_002109"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDI6365549.1"
                     /translation="MSATKLTRREQRARAQHFIDTLKGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="POA77_002110"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="POA77_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDI6365550.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="POA77_002111"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="POA77_002111"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDI6365551.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="POA77_002112"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="POA77_002112"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI6365552.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="POA77_002113"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="POA77_002113"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDI6365553.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /locus_tag="POA77_002114"
     CDS             22607..23197
                     /locus_tag="POA77_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDI6365554.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="POA77_002115"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="POA77_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDI6365555.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /locus_tag="POA77_002116"
     CDS             23669..24364
                     /locus_tag="POA77_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDI6365556.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24797)
                     /gene="zraP"
                     /locus_tag="POA77_002117"
     CDS             complement(24366..24797)
                     /gene="zraP"
                     /locus_tag="POA77_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDI6365557.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGMGYGGCGGGGHMGMGHW"
     gene            25035..26411
                     /gene="zraS"
                     /locus_tag="POA77_002118"
     CDS             25035..26411
                     /gene="zraS"
                     /locus_tag="POA77_002118"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDI6365558.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26408..27733
                     /gene="zraR"
                     /locus_tag="POA77_002119"
     CDS             26408..27733
                     /gene="zraR"
                     /locus_tag="POA77_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDI6365559.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27730..29019)
                     /gene="purD"
                     /locus_tag="POA77_002120"
     CDS             complement(27730..29019)
                     /gene="purD"
                     /locus_tag="POA77_002120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI6365560.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29031..30620)
                     /gene="purH"
                     /locus_tag="POA77_002121"
     CDS             complement(29031..30620)
                     /gene="purH"
                     /locus_tag="POA77_002121"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI6365561.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31236..>31324
                     /locus_tag="POA77_002122"
     rRNA            31236..>31324
                     /locus_tag="POA77_002122"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7419 a         8101 c         8552 g         7252 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtggggg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagc
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttcaacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gttcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcgac
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gcctttcagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggc gctctgtttc tcactgcctg
    23761 tagtcacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc
    24481 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    24541 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc
    24601 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    24661 gtctgctgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    24721 gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    24781 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    24841 ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    24901 gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    24961 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc
    25021 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    25081 gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc
    25141 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    25201 ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc
    25261 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    25321 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    25381 attccccgca ggaaatgcag cagttacatc ctggagatga agaagcgtgg cggcggatcg
    25441 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    25501 ctggaatgca ccggatgcgc catatgcagc agtatgccgc aacaccacaa gcaattttca
    25561 tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    25621 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc
    25681 gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg
    25741 tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaatcca ctttcctcaa
    25801 ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    25861 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagttgctgg
    25921 aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc
    25981 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    26041 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    26101 atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggtca
    26161 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag
    26221 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg
    26281 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc
    26341 aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    26401 cacaaggatg acgcacgata atatcgatat tttggtggtg gatgatgaca ttagccactg
    26461 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    26521 gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg
    26581 aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat
    26641 tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    26701 tggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga
    26761 aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca
    26821 gttcggtatg gtcggtaaaa gctcggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    26881 cgcgccatcg gaagccacgg tactgatcca cggcgattcc ggcaccggta aagagctggt
    26941 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    27001 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt
    27061 tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt
    27121 tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca
    27181 ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat
    27241 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    27301 ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    27361 tatccctctg ctggctggcc attttctgca gcgctttgcc gagcgtaatc gcaaggcggt
    27421 aaaaggtttt acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat
    27481 tcgtgagctg gaaaacgcgg tggaacgggc agtggtgctg ctgaccgggg aatatatttc
    27541 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga
    27601 tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcactgg agaaaacggg
    27661 cggcaacaaa actgaagccg cccgtcagtt agggatcacg cgtaaaacgt tattggcaaa
    27721 actgtcgcgt tagttctgct cgcgttcgat agcacgccag ccgatatctt tccggcagaa
    27781 gcagtcgtcc cagtggatat cggtcattaa ggcataggcg cgtttctgcg cttctgccac
    27841 ggtatgaccc agcgcggtga cgcacagtac gcgtccgccg ttggttacca cctgctcgtc
    27901 atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag
    27961 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    28021 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    28081 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    28141 atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag
    28201 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    28261 ccagataata cgttccatgg tgcgctgatg aacgtcatcg gttactaccg gcgcggggga
    28321 gtaagcgccc atcccgccgg tgtttggtcc ggtatcttta tcgcctacgc gtttgtgatc
    28381 ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc
    28441 ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc
    28501 cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc
    28561 tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata
    28621 cgccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg
    28681 ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg
    28741 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc
    28801 ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg
    28861 gatatcggtc acgccaatag caacgttttg cagcgcgggt tccagtgcag tgcctgcatt
    28921 acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc
    28981 gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg
    29041 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact
    29101 tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg
    29161 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact
    29221 tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    29281 tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc attggatttc
    29341 acgaacttcg ccactttcca gcagaacagc gcatcgcgca gttcctgttc ggtcggctga
    29401 cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc
    29461 agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg
    29521 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    29581 gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca
    29641 tctagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    29701 gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc
    29761 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    29821 tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag
    29881 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    29941 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct
    30001 tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg
    30061 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    30121 agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    30181 acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata
    30241 tcgatgttct caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac
    30301 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    30361 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    30421 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct
    30481 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    30541 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    30601 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    30661 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    30721 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    30781 attttgacag tggttaccgt gggaaaaata cagaaattac attgatattg tggataactc
    30841 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30901 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgcctcca tcgacacggc
    30961 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    31021 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg agctgaaagc
    31081 cgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    31141 gattcttaac gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa cacgtaattc
    31201 attacgaagt ttaattcttt gagcatcaaa cttttaaatt gaagagtttg atcatggctc
    31261 agattgaacg ctggcggcag gcctaacaca tgcaagtcga acggtaacag gaagcagctt
    31321 gctg
//
LOCUS       DAMZII010000048        30465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZII010000048 DAMZII010000000
VERSION     DAMZII010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.048"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..628
                     /locus_tag="POA77_002123"
     CDS             <1..628
                     /locus_tag="POA77_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI6365562.1"
                     /translation="SMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAG
                     DTYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDPEELA
                     NIFNKKHKEHMDDRFIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTK
                     QKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            890..2512
                     /locus_tag="POA77_002124"
     CDS             890..2512
                     /locus_tag="POA77_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI6365563.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLNEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2878..3945
                     /locus_tag="POA77_002125"
     CDS             2878..3945
                     /locus_tag="POA77_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6365564.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3942..6677
                     /gene="rbbA"
                     /locus_tag="POA77_002126"
     CDS             3942..6677
                     /gene="rbbA"
                     /locus_tag="POA77_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDI6365565.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6677..7801
                     /locus_tag="POA77_002127"
     CDS             6677..7801
                     /locus_tag="POA77_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6365566.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7860..8135
                     /locus_tag="POA77_002128"
                     /pseudo
     CDS             7860..8135
                     /locus_tag="POA77_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8132..8491
                     /locus_tag="POA77_002129"
     CDS             8132..8491
                     /locus_tag="POA77_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDI6365567.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8611..9012)
                     /gene="nikR"
                     /locus_tag="POA77_002130"
     CDS             complement(8611..9012)
                     /gene="nikR"
                     /locus_tag="POA77_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDI6365568.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9018..9824)
                     /gene="nikE"
                     /locus_tag="POA77_002131"
     CDS             complement(9018..9824)
                     /gene="nikE"
                     /locus_tag="POA77_002131"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDI6365569.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9821..10585)
                     /gene="nikD"
                     /locus_tag="POA77_002132"
     CDS             complement(9821..10585)
                     /gene="nikD"
                     /locus_tag="POA77_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDI6365570.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(10585..11418)
                     /gene="nikC"
                     /locus_tag="POA77_002133"
     CDS             complement(10585..11418)
                     /gene="nikC"
                     /locus_tag="POA77_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDI6365571.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11415..12359)
                     /gene="nikB"
                     /locus_tag="POA77_002134"
     CDS             complement(11415..12359)
                     /gene="nikB"
                     /locus_tag="POA77_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDI6365572.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12359..13933)
                     /gene="nikA"
                     /locus_tag="POA77_002135"
     CDS             complement(12359..13933)
                     /gene="nikA"
                     /locus_tag="POA77_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDI6365573.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14044..14631)
                     /gene="acpT"
                     /locus_tag="POA77_002136"
     CDS             complement(14044..14631)
                     /gene="acpT"
                     /locus_tag="POA77_002136"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDI6365574.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14686..15735)
                     /locus_tag="POA77_002137"
     CDS             complement(14686..15735)
                     /locus_tag="POA77_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6365575.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15867..17084
                     /locus_tag="POA77_002138"
     CDS             15867..17084
                     /locus_tag="POA77_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365576.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLALPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17088..17645)
                     /gene="dcrB"
                     /locus_tag="POA77_002139"
     CDS             complement(17088..17645)
                     /gene="dcrB"
                     /locus_tag="POA77_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDI6365577.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGNKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17718..18383)
                     /locus_tag="POA77_002140"
     CDS             complement(17718..18383)
                     /locus_tag="POA77_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDI6365578.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            18604..18849
                     /gene="tusA"
                     /locus_tag="POA77_002141"
     CDS             18604..18849
                     /gene="tusA"
                     /locus_tag="POA77_002141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDI6365579.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18951..21149)
                     /gene="zntA"
                     /locus_tag="POA77_002142"
     CDS             complement(18951..21149)
                     /gene="zntA"
                     /locus_tag="POA77_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDI6365580.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPENATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21223..21849)
                     /locus_tag="POA77_002143"
     CDS             complement(21223..21849)
                     /locus_tag="POA77_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDI6365581.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21990..22349
                     /locus_tag="POA77_002144"
     CDS             21990..22349
                     /locus_tag="POA77_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDI6365582.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22352..22621)
                     /locus_tag="POA77_002145"
     CDS             complement(22352..22621)
                     /locus_tag="POA77_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDI6365583.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22611..23207)
                     /gene="rsmD"
                     /locus_tag="POA77_002146"
     CDS             complement(22611..23207)
                     /gene="rsmD"
                     /locus_tag="POA77_002146"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDI6365584.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23357..24853
                     /gene="ftsY"
                     /locus_tag="POA77_002147"
     CDS             23357..24853
                     /gene="ftsY"
                     /locus_tag="POA77_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI6365585.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24856..25524
                     /gene="ftsE"
                     /locus_tag="POA77_002148"
     CDS             24856..25524
                     /gene="ftsE"
                     /locus_tag="POA77_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDI6365586.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            25517..26575
                     /gene="ftsX"
                     /locus_tag="POA77_002149"
     CDS             25517..26575
                     /gene="ftsX"
                     /locus_tag="POA77_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDI6365587.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26820..27674
                     /gene="rpoH"
                     /locus_tag="POA77_002150"
     CDS             26820..27674
                     /gene="rpoH"
                     /locus_tag="POA77_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDI6365588.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27946..29049
                     /gene="livJ"
                     /locus_tag="POA77_002151"
     CDS             27946..29049
                     /gene="livJ"
                     /locus_tag="POA77_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDI6365589.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            29199..29465
                     /locus_tag="POA77_002152"
     CDS             29199..29465
                     /locus_tag="POA77_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI6365590.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            29472..29999
                     /locus_tag="POA77_002153"
     CDS             29472..29999
                     /locus_tag="POA77_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6365591.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(29996..30379)
                     /gene="panM"
                     /locus_tag="POA77_002154"
     CDS             complement(29996..30379)
                     /gene="panM"
                     /locus_tag="POA77_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDI6365592.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7434 a         7711 c         8289 g         7031 t
ORIGIN      
        1 ttctatgcga aatatggagg gtaataaaga tagccgtatt aataaaaatg tcgtcgttct
       61 ggctggaaat catgaaatca attttaatgg taactatact gcacgactag caaatcataa
      121 actctctgcc ggtgatacgt ataacttaat taaaacgctc gatgtttgta attatgactc
      181 agaaaggcaa gttttaacca gtcatcacgg aataataaga gatgaagaaa aaaaatgtta
      241 ttgtctcggt gctttgcagg ttccatttaa tcagatgaaa aatcctactg atcctgagga
      301 attagccaat atttttaata aaaaacataa agaacacatg gatgatcgtt ttatccatct
      361 aattagaagc aatgctatag catccacacc tgtatatgac aattatttta ataacactac
      421 tgctttcaga ccaaaacctg aagatatctt caaatgtgga cagacgctca aaaaaactaa
      481 acagaaatat ggccattatg gattaggggt agatcagcat caaaaaattg ataattatac
      541 catggggcta aattcctgga agatagctcc taacgaacgt ggtgataaaa aaggtgtccc
      601 agggcttagt tgttttcaac cacattaaaa ctccatgagt gctgtggtat atctttttaa
      661 tcctgccagt atttcatagg ttttcttaat cgattgtaga ggcatgttct gatgacgcct
      721 ctacaatctt tagggacttt ctctgctatg agataagaaa tttcatatta tgctttaagg
      781 ctgaatgatt tgtgcaaaat ataattattt ttccatcgaa catgaggtgt atgtcataag
      841 attaaatgtt aactatgcat tcctcgcggt gaattatatg aggtaaaaga tgaaaattgg
      901 aactgtggca ggcactaacg acagtacaac gacaatcgca acaaatgata tggtgcaaga
      961 gcatgttact aacttcacta aagaactgtt tgggtatatt gcaaatggta taggtgatga
     1021 tatatcaagt attgccagga caatgctcgg tgaagttgtg gaaaaaattg atgattggca
     1081 aatagaacgt ttccaacaat ctattcgaga tgacaaaatt tcatttacca tacaaacgaa
     1141 tcattcagaa aaatatagta tgctatcggg catgcgcgct catattctac gtagggataa
     1201 taattatcag tttattgtga caattaatag tataaattac ggctgctatc ttgataatac
     1261 cgatattaat tggtgcagca ttgtttattt acttaataat atgacagtaa atgatgatgc
     1321 taatgatgtt gcagttactg agtcgtacaa acccatctgg aactggaaaa tatcgcaata
     1381 caatgtattt gatattaaat tcgaaacaat gataaaaccc cagttcgctg acagaaccta
     1441 tttctccaat tgttcacctg ttgatcccac aagtaccaga ccaacctatt ttggcgatac
     1501 cgatggctca gtcggtgcag ttttgtacgc gctttttgca acgggacatt tgggaatcat
     1561 ggctgaagga gaaaatttcc ttagccagtt gcttaatata gaagatgaag ttctcaacgt
     1621 attgctaagg gagaacttta atgagcaact tgacactaat gttaatataa taatcagtat
     1681 cctgaacaga agagacattg ttttagaaag tcttcagcct tatttggtta ttaataaaga
     1741 cgcagtcacg ccatgtacgt ttctgggcga ccagactggc gatcgattta gtaatatctg
     1801 tggcgataaa tttatcatcg atttattaaa acgcataatg agtatcaatg aaaacgttca
     1861 tgtattagct ggaaatcatg aaacgaattg caatggtaac tatatgcaga acttcacgcg
     1921 catgaaacca cttaatgagg atacctacgc tgggataaaa gattatcctg tatgtttcta
     1981 tggccccaaa tacaaaataa tggcaaatca tcatgggata acttttgatg atcagcgaaa
     2041 acgttacatt ataggaccga taacagtctc tagtgatgaa atgaccaatg ctctcgatcc
     2101 ggtcgaatta gcggcaatta tcaataaaaa gcaccatgct gtaattaatg gcaaaaagtt
     2161 taaaaatagc cgggctatct cttgtcgatc ctttaatcgt tatttttctg tttcgacaga
     2221 ttacagacct aaacttgaag cactattagc atgctcacaa atgttaggta taaatcaagt
     2281 tgttgcacat aacggtaatg gtggaagaga atgtataggt gaaacgggga cagttttagg
     2341 tcttaacgcc cgtgacagta aacatgctgg aagaatgttc agcatgcata attgccaaat
     2401 taaccctggt gcagggcctg agataaccac tccctggaag tcttaccagc atgaaaaaaa
     2461 cagaaatgga cttatgcctt tgattagaag gcgcacaatg ttacagctct gaaccataga
     2521 caacatgaat attaatgtta ctatctggtt gagactgttg ataaaacgta aaaaggaatg
     2581 ctctgttcct gcaactgaac aggcgtgaaa cgcgatccaa caggatggat accgttttgt
     2641 cagccagttt tatagatcac acttatgagt atgcgtgttt catttacggc caggaatgct
     2701 gatgatgaca acattactac ccgttttcac taagccttcc ccgttggcgc tcaatgctct
     2761 gcgcgctggt cgtatttgtc gtttccttct tatcccggat ggaagaatcc gctgaacgtc
     2821 gttcggcggc agttgtaaca cgcgtcgttt gcttacaggg attatggcgg gccgattatg
     2881 gataagagta agcgccatct ggcgtggtgg gttgtcgggg cactggcggt ggctgccgtc
     2941 gtggcgtggt ggctgttgcg cccggcgggt gtgccggaag gctttgctgt cagcaatggg
     3001 cgcattgaag cgacggaagt ggatatcgcc agcaaaattg ccgggcgtat cgacaccatt
     3061 ctggtgaaag aaggccagtt tgttcgcgaa ggtgaagtgc tggcgaagat ggatactcgc
     3121 gtgttgcagg aacagcgact ggaagccatc gcgcaaatca aagaggcaca aagcgccgtt
     3181 gccgccgcgc aggctttgct ggagcaacga caaagcgaaa ctcgcgccgc gcagtcgctg
     3241 gttaatcaac gccaggcaga actggactcc gtagcaaaac gtcatatgcg ttcccgttca
     3301 ctggcccaac gaggggctat ttctgcgcaa cagctggatg acgaccgcgc cgccgctgag
     3361 agcgcccgag ctgcgctgga atcggcaaaa gctcaggttt ctgcttctaa agccgctata
     3421 gaggcggcac gtaccaatat cattcaggcg caaacccgcg tcgaagcggc acaagccact
     3481 gaacggcgca ttgccgcaga tatcgatgac agcgaactga aagccccgcg tgacggacgc
     3541 gtgcagtatc gggttgccga gccaggggaa gtgctggcgg caggcggtcg ggtgctgaat
     3601 atggtcgatc tcagcgacgt ctatatgacc ttcttcctgc caaccgaaca ggcgggcacg
     3661 ctgaaactgg gtggtgaagc ccggctgatc ctcgatgccg cgccagatct gcgtattccg
     3721 gcgaccatca gttttgtcgc cagtgtcgcc cagttcacgc caaaaaccgt cgaaaccagc
     3781 gatgaacggc tgaaactgat gttccgcgtc aaagcgcgta tcccaccgga attactccag
     3841 cagcatctgg aatatgtcaa aaccggttta ccgggcgtag cgtgggtgcg ggtgaatgaa
     3901 gaacttccgt ggcctgacga cctcgtggtg aggttgccgc aatgacgcat ctggaactgg
     3961 ttcccgtccc gcctgtcgcg caactggcgg gcgtgagcca gcattatgga aaaaccgttg
     4021 cgctgaacaa tattactctc gatattccgg cccgctgtat ggtcgggctg attggtccgg
     4081 atggcgtcgg gaagtcgagc ttgttgtcgt tgatttccgg tgcccgcgtc attgaacagg
     4141 gcaatgtgat ggtgctgggc ggcgatatgc gcgacccgaa gcatcgccgc gacgtctgcc
     4201 cgcgcatcgc ctggatgccg caggggctgg gcaaaaatct ctaccacacc ttatcggtgt
     4261 atgaaaatgt cgattttttc gctcgcttgt tcggtcacga taaagcggag cgggaagtcc
     4321 gaattaatga gctgctgacc agcaccgggt tagcaccgtt tcgcgatcgt ccggcaggta
     4381 aactctccgg cgggatgaag caaaaactgg ggctgtgctg cgcgttaatc cacgacccgg
     4441 aactgttgat ccttgatgag ccaacaacgg gggttgaccc gctctcccgc gcccagttct
     4501 gggatctgat cgacagtatt cgccagcggc agagcaatat gagcgtgctg gtcgccaccg
     4561 cctatatgga agaggccgaa cgcttcgact ggctggtagc gatgaatgcc ggagaagtgc
     4621 tggcaactgg cagcgccgaa gagctgcggc agcaaacgca aagcgccacg ctggaagagg
     4681 cgtttataaa tctgttaccg caagcgcaac gccaggcgca tcaggcggta gtgatcccac
     4741 cgtatcaacc tgaaaatgca gagattgcca tcgaagcgcg cgatctgacc atgcgttttg
     4801 gttccttcgt tgccgttgat cacgtcaatt tccgcattcc acgcggggag atttttggtt
     4861 ttcttggatc gaacggctgc ggtaaatcca ccaccatgaa aatgctcacc ggactgctgc
     4921 ccgccagcga aggtgaggcg tggctgtttg ggcaaccggt tgatccaaaa gatatcgata
     4981 cccgccgtcg ggtgggctat atgtcgcagg cgttttcgct ctataacgaa ctcaccgtgc
     5041 ggcaaaacct tgagttacat gcccgtttgt ttcacatccc ggaagcggaa attcccgcgc
     5101 gagtggctga aatgagcgag cgttttaagc tcaacgacgt tgaagatgtt ctgccggagt
     5161 cattgccgct cggcattcgc cagcggcttt cgctggcggt ggcggtgatt catcgcccgg
     5221 agatgttaat cctcgatgaa cctacttctg gcgtcgatcc ggtggcgagg gatatgttct
     5281 ggcagttgat ggtcgatctc tcgcgccagg acaaagtaac catttttatc tccacccact
     5341 ttatgaacga agcggaacgt tgcgaccgca tctcactgat gcacgccgga aaagtgctcg
     5401 ccagcggtac accgcaggaa ctggttgaga aacgcggagc cgccagtctg gaagaggcat
     5461 ttatcgccta tttgcaggaa gcggcaggac agagcaacga agccgaagcg ccgcccgtga
     5521 tacacgacac cacccacgcg ccgcgtcagg gatttagcct gcgccgtctg tttagctaca
     5581 gccgccgcga agcactggaa ctgcgacgcg atccggtacg ttcgacgctg gcgctgatgg
     5641 gaacggtgat cctgatgctg ataatgggtt acggcatcag tatggatgtg gaaaacctgc
     5701 gctttgcggt gctcgaccgc gaccagaccg tcagtagcca ggcgtggacg ctcaatctct
     5761 ccggttcccg ttactttatc gaacagccgc cgctcaccag ttatgacgag cttgatcgtc
     5821 ggatgcgtgc gggcgatata acggtggcaa ttgagatccc tcccaatttc gggcgcgata
     5881 tcgcgcgtgg tacgcctgtg gaactcggcg tctggatcga cggagcgatg ccgagccgtg
     5941 ccgaaacggt aaaaggttac gtgcaggcaa tgcaccagag ctggctacag gatgtggcga
     6001 gccgacaatc cacacccgcc agccaaagcg ggctgatgaa tattgagacg cgctatcgct
     6061 ataacccgga cgtaaaaagc ctgccagcga ttgttccggc ggtgatcccg cttctgctga
     6121 tgatgatccc gtcaatgcta agcgccctta gcgtggtgcg ggaaaaagag cttgggtcga
     6181 ttatcaacct ttacgtgacc cccaccacgc gtagtgaatt tttgcttggt aaacagttgc
     6241 catacatcgc gctggggatg ctgaactttt tcctgctctg cggcctgtcg gtgtttgtgt
     6301 ttggcgtacc gcataaaggc agtttcctga cgctcaccct ggcggcgctg ctgtatatca
     6361 tcattgccac cggaatgggg ctgctgatct ccacctttat gaaaagccag attgccgcca
     6421 ttttcggaac ggcgattatc acgttgatcc cggcgacaca gttttccggg atgatcgatc
     6481 cggtagcttc gctggaaggg cctggacgtt ggatcggcga ggtttacccg accagtcatt
     6541 ttctgactat cgcccgcgga acgttctcga aagcgctgga tctgactgat ttgtggcaac
     6601 tttttatccc gttgctgata gccatcccgc tggtgatggg cttaagtatc ctgctgctga
     6661 aaaaacagga gggatgatgc gccatttacg caatattttt aatctgggta tcaaagagtt
     6721 gcgcagtctg ctcggtgata aagcgatgct gacgctgatt gtcttctcgt ttacggtgtc
     6781 ggtgtattcg tcagcgaccg ttacgccagg atcgttgaac ctcgcgccga tcgccattgc
     6841 cgatatggat caatcgcagt tatcgaaccg gatcgttaac agcttctatc gcccgtggtt
     6901 tttgccaccg gagatgatca ccgccgatga gatggatgcc ggactggacg ccgggcgtta
     6961 tacctttgcg ataaatattc cgcctaattt tcagcgtgat gtcctcgccg gacgccagcc
     7021 ggatattcag gtgaacgtcg atgccacgcg catgagccag gcatttaccg gcaatgggta
     7081 tatccagaat attatcaacg gtgaagtgaa cagctttgtc gcgcgctacc gtgataacag
     7141 cgaaccgttg gtatcgctgg aaacccggat gcgctttaac ccgaaccttg atcccgcgtg
     7201 gtttggcggg gtgatggcaa tcatcaacaa cattaccatg ctggcgattg tattgaccgg
     7261 atcggcgctg atccgcgagc gtgaacacgg cacggtggaa cacttgttgg tgatgccgat
     7321 aacgccgttt gagatcatga tggcaaaggt ctggtcgatg gggctggtgg tgctggtggt
     7381 gtcgggatta tcactggtgc tgatggtgaa aggcgtgctg ggtgtaccga ttgaaggctc
     7441 gatcccgctg tttatgctgg gcgtggcgct cagtctgttt gccaccacgt caatcggcat
     7501 ttttatgggg acgatagcgc gttcaatgcc gcaactgggg ctgctggtga ttctggtgct
     7561 gctgccgctg caaatgcttt ccggtggatc cacgccgcgc gaaagtatgc cgcagatggt
     7621 gcaggatatt atgctgacca tgccgacgac acactttgtt agcctcgcgc aggccattct
     7681 ctaccggggt gccggattcg aaatcgtctg gccgcagttc ctgacgctga tggcaattgg
     7741 cggcgcgttt ttcaccatcg cgctgctgcg attcaggaag acgattggga caatggcgta
     7801 aggaagggaa acttgacttc atactgtgat gtcataaagt gaagtcataa gggaatgtca
     7861 tgtaaacaca ggtacaggct ttacgaaaga aacaaaaaaa cacactggat cagctattta
     7921 aatcaccttt acctcaagga atcaaatgga ctgatataga atctctggtt aaagcgttag
     7981 gcggagaaat taaggaaggt agggggtcgc gctgtaagtt catactaaat atgagcgttg
     8041 cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc
     8101 gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat
     8161 ggagattgcc gggcaacctg ctgttatcac ttacgtgcca gaactgaatg catttcgtgg
     8221 caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca
     8281 aaaagaaggg gaactttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga
     8341 gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag
     8401 tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga
     8461 aacactgaat gagcgtttga atcatttata aattttagcg gcgtacatta cgcagaatat
     8521 ctgcctttac aggatctatc tatgcaactg aacttaattt tataaattaa gttcagttaa
     8581 tatcttttct ttcctcgttt cagaaataac tcaatcttcc ttcggcaagc actgcaaatg
     8641 cccgtgccgc acgccgcgct gggcgataac gtcatcggca aaatgctgca cgtcgcccat
     8701 atcacctttc agcacggcga tttccagaca gtcgtcgtga ttgatatgca catgcagcgt
     8761 ggcgacggag agatcgtggt gatggtgctg ggtggagaca atgcggctgg ctaagtcgcg
     8821 tttttcgtgt tcatacacat aggagagcac cgcgaaacct tgcgtgccgt gctgctgagt
     8881 ggcctcctgg gccagagcgc tacgcagaat atcgcggata gcttcggaac gattgttata
     8941 accacgacgc tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt
     9001 gactcgttgc atttgcgtta aaccttttct gtggtgcgac ggcgcacggg gaatgcgggt
     9061 aataccgcgt tttgtagcac acgtccggcg tcagaggaaa aggttaattt ctctcccacc
     9121 acctgggttt cgacgatttg tccgttgtcc atcaccatca cccgctgaca gaagcgttcc
     9181 accaggcgta agtcgtgggt gatgaacagg caggcggtgc caaactgttg ttgtagcttt
     9241 ttcagcaggc gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac tgcttcatcc
     9301 agaatcagta gtttcggttc gaccgccagc gcgcgagcca ggcagacgcg ctggagctgg
     9361 ccgccgctta actgcggtgg gcgcttgtcg agcacgctgt cgtcgagatc gaccgccttc
     9421 agcatttcgc tggcgcgcgc cagttgttcg gattttttca gtgatagcag gtggcgcatc
     9481 ggttcacgca ggatctcgcg cacggttttg cgcggattca cggcgctgat ggagtcctga
     9541 aataccatct gaatatcgcg gcggaacgct ttacgctggg cgcgattgag tttcgccagc
     9601 ggttcgccgc gccagctaat attcccttgt gagggagact ctaagcctac cagcagccgc
     9661 gcgagggtgc tttttccgca gccgctgcgt cccagcaggg cgacggtttc gccgcttttc
     9721 agggtcaggg aaacgttatt cagcaccgcc tgatgttgat gttttccgct aaatccaccg
     9781 tgggcatagt gatgggaaag gccgcagacg ttaagtaagg tcatgatgcc agctccatac
     9841 cgtagagggc gagatgagcg gaaaccaggc tgcgcgtcac cgtatgtttg ggggcgttaa
     9901 acagcgtttc tacatcgccc tgttcgacaa ttttaccttg tgacatcacc gccacgtcat
     9961 ccgccagacg cgccacaacg cccatatcat gggtgaccag cagcattccc ggcgcttgtt
    10021 tttgcataat gctttccagc agatcgagga tgcgcgcctg tgctaccacg tcgaggtcgg
    10081 tggtcggttc atcggcgatg ataaacggtg attcacacag caccgccatc gcaatcatca
    10141 tgcgctgcaa catgccgccg ctcatctcga acgggtacag cttcagcacg cgcgcggcgt
    10201 tttccagccc caccgcttct atggcagcgg taagcgtagc gtcatcggcg ggtttcccta
    10261 acgccaggca ggtttcccgc gcgtgggtgt gcatggtgtg cagtggatta aaggcgctgc
    10321 gcgggttctg catgatggtg gcaattttga tgccgcgcag ggcgcaaggc gaaaccggtt
    10381 tgccatcggc taaaatttcc cccgccgtct ggcgaacgcc agcgggcaaa atgcccagcg
    10441 tcgcggcgca ggttaatgat ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc
    10501 cgcgttgcag ggttaacgat acaccgtgta ccagcggctg cgcggcctgt agcgcgatat
    10561 tacgtagttc aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat gaggatccag
    10621 atgatcgcgc agtgcgtcac ccaccaggtt aaaggccatc acgctgataa acagcgccag
    10681 ccccggccag aacatttgca gcggctgggt ccagatatac tggcgcgcgt cgttaatcat
    10741 cacgccccat tcggcggtcg gcgcggtcac accgaggcca aggaaagaca tccccgcgac
    10801 gtgcagcatc atatggccga tatccagcgt tgccagcacc agcagcgaag ggatcaccgc
    10861 gcctgccaga tgatcgacaa acacccgcac atggcccgca ccggaaagcc gtgacgccag
    10921 cacaaactcg cgttggcgta gtgaaatcac caggctgcgc accatgcgtg cataccacgc
    10981 ccagtgcgac agggcgatgg cgataattac gttggtcagc ccggtgccga gcacgccaac
    11041 cataaagaac gacagaatcg aggtcgggaa ggtcataaac atatcggcga cgcgcatggt
    11101 ggcctgatca acgcgtccgc caatcagtcc ggcgctgccg ccaatcacca gccctaatgt
    11161 cagcaccagc agcaggcagg ccattaccga accgagcgac acgcgggtcg ccgccatcag
    11221 ccgcgagaaa atatcacgcc ctaagtgatc ggtgcccagc cagtgctgcg catccggcga
    11281 aagcaggcgc gacggcagat caatagcctg agggtcatac ggcagccacc actggctggt
    11341 aagcgcaatc agcgccagca gggcgataat gatcagcgcc aggcgtaccg accagcggga
    11401 agagaggaaa aagttcacgc gtgcgctcct tcatgacgac gaatgcgcgg gtccagcgcg
    11461 gcgttgagca aatcgacaat caaattacag accacaaaaa ccaccaccat catcagcgta
    11521 aagcactgga tcaccggata gtcacggtta aaaatcgccg acaccgcata gcgcccgacg
    11581 cccggccagg caaagatgtt ttcgataatc atcgtcccgc cgatcagttc gccgatgtgc
    11641 atccccacgg cggtgatcat cggcagcgag gcattgcgca ggatgtggcg acgttcggtc
    11701 tgtttgtcgt tcaggccgcg cagacgcgcc caggtgacgt gacgctgacc ggcgacgtcc
    11761 agcatactgg cgcgcagtaa acgcgcgtta atcgccagcg acataaaggc aatggaaacc
    11821 gcaggcaaaa tgatgtgctg ccagccgccg taacccatcg cgggtagcca ttgcagatac
    11881 accgaaaacg ccatcaccag taaaaacgcc agccagaagt taggcatcga cacgccaaga
    11941 aacgcgatga aacgtacggc gaaatccggc agacggtcgc ggtggcgcgc cgcccagata
    12001 ccgagcggta cggaagtgag cagaattaat accagcgccg cacctgcaag ttccagcgtg
    12061 gcgggcagga agttcagcat atcgtccagt accgggcgtt ggctggcgaa tgagatacca
    12121 aagtcaagat gcagcgcctt ccacaaccag gtgccgtact ggacgtacag cggctgatcc
    12181 agtcccagca tggtgcgggt agaggccagc atctccggcg tcggcggcag gttagacaga
    12241 cgcaaataat cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc
    12301 gaggcggcga gcaccatcgg gatcagcagc agaaagcggc gtaatacgta acgcaacatt
    12361 aaggtttcac cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga
    12421 tgttacccag ctccggtttt gataccacca tcatcgagat gtagctgata ggcagataaa
    12481 ccgcctcgtc atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt tgcgtttcgt
    12541 catgggtcgc cagcacttcg ccgatctctt tatcaatcag cggcttgtcg gctaatcctt
    12601 gctgtgcctg gaagtcagcg tgcgacggta cgcgcattga actcaggaag gcgtgtggat
    12661 cgtatggcgc gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag
    12721 catagatact gctctcttct tcgccaatca gcgagacatc tgcgccaatc tggcgcatat
    12781 cagcctgaat gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt
    12841 caatgcgcag cggctgacca tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac
    12901 cggctttttc cagcaactct ttcgctttct gcggatcgta ctggcgcggt ttcaggccga
    12961 gattagcgta aggcacagaa ggggcaaaca gggtgtcggc gacctgctgg gtgccatata
    13021 acgcgttatc aatcagcgat tttttgttta ccgcgtaatt aagagcttca cgtactgcca
    13081 gctcgttggt gggggctttg gcggtattga gcgccagcat cacggtttcg atcggctgtg
    13141 acagttgggt gtggtaagcc ggattctggc taaagcgggc gaaggtatcg agcggtaata
    13201 acccttcgtt tccgtacagc aggtcgatat cgccagtttc aaacgccacc gcgcgggtag
    13261 tcgggtccgg gatgacgtta aaggtgatct ttttaatcgc tggcttttcg ccccagtagt
    13321 tttcgttacg gacgaagaca tcgtactgat tcagtttcga ttcctgcaaa atccacggtc
    13381 cggtgccaat cggcgcttta attccgttca tggtttcatg gtttttaaac tgcgagggag
    13441 cgataaaacg gaaaggacgg ggcagggcca gttcttgcag gaaaggatag taggcgcttt
    13501 tcagggtaat ttgcagctct gttttactga gtgctttaac atcaacaatc tggtttgcca
    13561 gctccagcca ggcgtgacgt tgacggttat cgagcactgc gcggaagttt tctgccgccg
    13621 cctcggcatc gaacggttca ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc
    13681 aggttttacc atcttctgaa tgagtccagc tttttgccag ccacgggatc accgaaccgt
    13741 ctgcctgata tttcaccaat ggttcataaa ccatgctctg ggcgaacatc tggttaggcg
    13801 tgtaaaggtg cgggtttagt ggcccgacat tcaccggcca ggcggtggtg atttcatctg
    13861 gtgcagcggc atggacgata aaagacgcac aagccagcag cgcaaataga gtgcggcgga
    13921 gtgtggagag catggtaacc ccaatggatt aaaatagatg gcggaaataa gtatgacgat
    13981 tttaagtatt cgtcatactg attacctgtt ctggatcaat agatgggcag tcgggtgggc
    14041 ggatcagtta actgaatcga tccattgcac actgtcggcg gtgagcgtaa agggagtagg
    14101 ggtgcagatc gccaggctta aattttcaag ctgacaatgg ctgactgaca gcgaggagtg
    14161 ataggtgctg tctacgctga cgatttgcca ggcgctgccg ccacgctgtt taacgatggc
    14221 ttctttgcgc gtccagatgc gccagaacat ttccagttgc tgatcaggat gcacggcgtc
    14281 catctcagcg tgttccccga ggctgaatac ggcgttcgcc agccagcgcc agttggcgcg
    14341 cgggcgaatc acttcgatat cgcagccgac ttcgccttca tcactcaaca gcagggcgat
    14401 atcgtcaccg ctatggctta agttgaacca tagcggcgtt tccggcgcaa atgcaggttt
    14461 gccttgttcg ccatagatga tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg
    14521 ccccgccagc cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg
    14581 cagtggagct gcgcttaagg tcgaaacttt ccccagaact atccgataca tatcagggcc
    14641 aacgtttaat ggaaaatgaa agtgcgtatc gtatcacttg tcgcctcatc ccggtaaccg
    14701 acttttcggt ctgcctggcc ccagtaaaat cgccagtttg ctaccgcctt tggtggtttc
    14761 catccagatt ttacacacac tggttaatgg caccgaaagt agcatcccta ccgggccgag
    14821 cagccatccc caaattaaca atgaaagaaa taccaccatg gtggacatcc ccaggcgatg
    14881 gcccatcatc cgtggctcta aaatattgcc gatgaccata tggacaacta aaaacaatgc
    14941 gccgaccaga atacattcgt aaataccatt aaacagcagc acctgaatca ttggcggtac
    15001 ggcggaaatt accgcgccga tattgggcac gtagttaagc aaaaacgcca gtactgccca
    15061 catcagcgca aactgcaccc ccatcagctc cagccccagc cagacgatga cacctgtcca
    15121 taaactgagt agcgtcttta acgccagata atgcgaaacg cctttaagtg cgcggtgtaa
    15181 tcctgcgatg tgaatctgtg ggttattcag cgcaaaacgc attttgtaag ggacgtggcg
    15241 cacttcaaac agcataaaaa ctacggtcat caccagcaaa agcacgctcg ccattgcccc
    15301 ggaaagcccg gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg
    15361 cagcattcgc tccggcgaca tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa
    15421 aagtttgcgc gtcagctcct tattaaactt cggcagcata gagataaatt cgttaaacga
    15481 tgccgccagt acgccgacca gcgcggttag tgtgatcagc atcaccacca ccacaatcgt
    15541 aatggcaacg gggcgtttta ctccccgtcg aataaaccag gtaacgagcg ggttgaggac
    15601 gatggcaaaa aacagcgcca gtaacagctg cacaatgata tctgccgctg cgtgaatgcc
    15661 cgcgaggatc actaccagcg aggccagctt gagcagaatg tgcatgcccg ttttatcggg
    15721 ttgaggggtt tccattgggg cttccttgtg actttttgta ttaagtgtag cgggagtcac
    15781 gccagcaata ttctgattct tactgctgat tttcacgtca gcacatggta aaaatgaaac
    15841 actgttgtaa aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg
    15901 gattgcgcct gaatttgcgc attgtctcca ttgtcatgtt taacttcgcc agctacctca
    15961 ccatcgggtt gccgctcgct gtattaccgg gctatgtcca tgatgtgatg ggctttagcg
    16021 ctttttgggc aggattggtt atcagcctgc aatacttcgc caccttgctg agccgccctc
    16081 atgccggacg ttacgccgat ttgctgggac ccaaaaagat tgtcgtcttc ggtttatgcg
    16141 gctgcttttt gagcggtctg ggatatctga cggcaggatt aaccgccagt ctgcccgtca
    16201 tcagcctgtt attactttgc ctgggacgcg tgatccttgg gattgggcaa agttttgccg
    16261 gaacgggatc gaccctgtgg ggcgttggcg tggttggctc gctgcatatc gggcgggtga
    16321 tttcgtggaa cggcattgtc acttatgggg cgatggcgat gggtgcgccg ttaggcgtcg
    16381 tgttttatca ctggggcggc ttgcaggcgt tagcgttaat cattatgggc gtggcgctgg
    16441 tggccatttt gttggcgctc ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc
    16501 cgtttcgcgc ggtgcttggg cgcgtctggc tgtacggtat ggcgctggca ctggcttccg
    16561 ccggatttgg cgtcatcgcc acctttatca cgctgtttta tgacgctaaa ggttgggacg
    16621 gtgcggcttt cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc
    16681 ctaacggcat taaccgtatc ggcggcttaa acgtagcgat gatttgcttt agcgttgaga
    16741 taatcggcct gctactggtt ggcgtggcga ctatgccgtg gatggcgaaa atcggcgtct
    16801 tactggcggg ggccgggttt tcgctggtgt tcccggcatt gggtgtagtg gcggtaaaag
    16861 cggttccgca gcaaaatcag ggggcggcgc tggcaactta caccgtattt atggatttat
    16921 cgcttggcgt gaccggacca ctggctgggc tggtgatgag ctgggcgggc gtaccggtga
    16981 tttatctggc ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa
    17041 aacggcctcc ggaacacgtc cctgaggccg cctcatcatc ttaaaactta ctgaataacc
    17101 agcgtattaa tgatgttttc tgcggttgtc tgcgcttttt gctgatcgtc agctggcagc
    17161 gtaatttgca tggttagcag ttgattaccc acgttaccca gaataacaga agagtacgcc
    17221 gtctggcctt tcgcggagat aatactgtct aactgctgca ttttgttacc tttcagctca
    17281 atggctttat tggttaccac ttgcagctgc ggatcgcggc tacgttgctg atcttccaga
    17341 cgcttcgcca gcaccgccag atcttctttc ggatcatcgc ccatgatgac gatgactgct
    17401 ttctgaccgg tggcgtcgga ccagacatgc atgttattgg cctgcgttcc cagcttaccg
    17461 ctctggtcgg tcatatccgc tggcagcgag aaacttaact tgccatcaag caggttgacg
    17521 ggattcccgg tagcgttact ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta
    17581 tcatcacagg ccgcaagccc cataaccagc aggccaattc cgacatattt aaccagattg
    17641 cgcattgact tcttcctttc gataaacggc cataacggct cattcatcca tcttatcaca
    17701 actctgataa cgaaccttta actcgcctgc aaagcgttga tttcggattt atctgccagt
    17761 cttttcaaca gcatattgag taatacgcca tacattggca ggaagaaaac gatactgatt
    17821 aacactttga aacagtaatc gaccagcgcg atttccatcc agtgttcagc cataaaggca
    17881 tccgggctac gccagaaggc aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac
    17941 agtgtggacg ctgtcggtgc cagccaccag cggcgactct gacgcaggcg gttaaaaacg
    18001 tgcacgtcga ggatctgtcc cagcgcgtag gccatgaaac tggcggtggc gatacgggcg
    18061 acaaacaggt tgaagtgggc gagtgcgccg aatccctgcc atgaacccat atagaatagc
    18121 gacgagatga cgtaggagat cagcaatgcc gggatcatca ccgcaaagat gatacgacgg
    18181 gccagcggtg cgccaaaaat acgtacggtc aggtcggtag cgagaaagat aaacggaaag
    18241 ctaaacgcgc cccaggtggt atggaaacca aaaatggaga cgggaagctg aaccagatag
    18301 ttactggagg tgatcaccag cagatgaaat aacgataacc agaacaacgc tttatagcgt
    18361 tgagtttgcg agaaaacgtt catattgtac ctttttgatt aaccattggg gtgagggaac
    18421 ccaatacgta cgacacgtct gttaccttaa cataataacg tgcttaccct tttttcgagc
    18481 cgccgcatga tactgcttta cgttgacaat gcaatggtta attttcacgc aatcgttaac
    18541 ctggtttgct tacggactca cagggcgtaa actagcgccg tttttttatg tgatgagaag
    18601 aaaatgaccg atctcttttc cagccctgac cacacactcg acgcacttgg cctgcgctgc
    18661 ccggaacccg taatgatggt gcgcaaaaca gtgcgcaata tgcagccagg tgaaacgttg
    18721 ctgattattg ctgacgatcc ggcgacgacc cgcgatatcc ctgggttttg tacctttatg
    18781 gaacacgaac tggttgccaa agagacggat ggactgcctt atcgttattt gattcgtaaa
    18841 ggcggttgat ggggaaagat tggcttcgat gccgcctttt cccctcaccc taaccctctc
    18901 cccagaggag cgaggggacc gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg
    18961 cgcaacaatc ttaacgcatt cgctgtcacc agcaccgtag cacccgtatc tgccagcacc
    19021 gccagccata gtccggtcat tcctaacagc gtggtgacga ggaagatccc tttcagcccc
    19081 agcgcaatag tgatgttctg gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt
    19141 tgcaccagac cgcgcaggtg gttatgggtt aatgctgcgt cggcggtttc cagcgccacg
    19201 tctgtgccgc tgcccattgc aatcccgatg gcggcagctt tcatcgctgg cgcgtcgtta
    19261 ataccgtcac cgaccatcgc cagcggcgca tgttgattca gctcggtcac cgctttgact
    19321 ttatcttccg gcaacaggcc cgctttaaac tccagcccca gctccccggc aattgccgcc
    19381 gctgcgcgtg gattatcgcc ggtgaggatc acccctttga cgcccagcgc gttcagttca
    19441 ctgatggcag ttgcagcatc ggcgcgcagg gtatcctgta acgcaatgac acccagcacg
    19501 tcatcgttac gtactaccag cactaccgtt tgcccggcgc tttccagttc gttaattaaa
    19561 ccagcaaatg catcagcggg atgtttcccg gcagcgcaaa tcaatacgcg ctcaccgtta
    19621 acctgcgctt caatgccaga cccgaccagc gcccgctgtg attcggcggc gggaatggcg
    19681 agtgcagcaa cctgtgcttc gcgcacgatg gcctgcgcca gtggatgcgt cgcgccttgc
    19741 tcgaccgccg ccgccagtgt cagcagttca gattcactaa tacccgttgc cggatgaatc
    19801 gcggtaacgc gcggtttacc gacggtcagc gtaccggttt tatcaaacgc cacctgggta
    19861 acacgaccta gctgctccag cgccgcaccg cctttaatta acgccccacg acgtgccgct
    19921 gctgccagcc cggaggtaat cgccgcgggc gtggagataa ctaacgcaca cgggcagcca
    19981 atcagcagca gcgtcaggcc tttataaatc cactcttgcc agctggcggc aaacagcagc
    20041 ggcggcacca gcgttaccag cagggcgacg gccataattg ctggcgtata aatgcggctg
    20101 aaacggtcga taaaccgctc aatgggagcg cgacgctctt cggcttcttc gatcagtttc
    20161 agaatccggt caatggcgct ggctcccggt tcggacaata cttccaacgt caccagacgg
    20221 tctacgctgg tggcgcctgc aggaacttta tcgcccgtcg cacgctccac cggaatggat
    20281 tcgccggtca gggcgctttc atcaaaactg gcaaagggtg agagcagttt accgtcggca
    20341 ggcaaacgcc cacccgccgc aacttcaatc acatcgccag ggcgcaggct gttaattgcc
    20401 acctcttcac gctcaccgtt acgcaggcgc gtggcgtttt ctggtttcag cgccattaac
    20461 gcgctgacgc cctgacgcgc gcggctggcg gcccaacctt ccagtcgttc accaatcaaa
    20521 aacagcagca acaccatcgc agcttcagcc gttgcgccaa taaacagcgc accaatagcg
    20581 gctacgctca ttaacgtttc aatggcgaag taactgcctg atttgatcag ccgtaatgcc
    20641 tgacgagcaa tcgggtacag cccaaccagc gtggtcgcga taaacgccag ttgcccgaac
    20701 ggatgattga actgctccag accccagctg attgccatca tcacgattag cgtaatcagc
    20761 gacagattct ctttcaggcg tgatgcttgc ggttcttcgg cggcctgttc atcgcgcagg
    20821 gaatagcctg ctttttgcac cgcagattca acctgggcac ggatgtcatt gtcggcatca
    20881 accaccagtt tttcggtggc gaacaacacc tgtacctgat tcacgcctgc aagctggcgc
    20941 acggcatttt ctaccttgcg cgcacaggcg gcgcagtcca tgccgctgac tttccagcta
    21001 tagcgggtgc cggagacgtt ttcagagaga gttggcgtgc tggaacatgc gccgtcgcag
    21061 caacagtcgt tggcgttctg taccgtggtt agcggtttga acgcagcaaa ttgaggggct
    21121 ttcttgccgt gattgtcagg agtcgacatg gcatcctccg gttaagtttt ttctcattaa
    21181 ccgaaggata cactctggag tcgactccag agtcaagttt tatcagagat acagcgagcg
    21241 gacgatcagg aagtgaccgg caaagtagca ggccgcagca atcgcgttat ccgcacggaa
    21301 gcgtcggcga tagtgactac ccagccagac aaagttactg ataaacagca acgaagcgcc
    21361 gacaaacgca gagagcgccg gagcggtcgg acggaagaac cacagttcac ctgccagcca
    21421 caccatcacc agcgtcatgc cgataaacgt gcagataggc caacgatact cttccagccg
    21481 cgtccagata atcgccagta acagcgcgcc cagaaccagc agcaccagtg gcagaggcca
    21541 gaagaaagag agcgtcatct gactggcgaa atagatggtg tacagcaggt gcgagaggaa
    21601 aaacgcgccg atggcgtaca tcagacgttg acgtggcaac agggttagcg catcgcccag
    21661 cagtgaggcg cacagccccg ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg
    21721 ccaggccagt aacagcagga gaagaagggt taatggttta aatacccagc gttgccaggt
    21781 tggcccacga tacgatgcat ccacagatag ccatgcggaa agacagacag cgataaacga
    21841 ccaaagcatc ttagttcctt gaattcgtta ttttttcgta agcggaatgc ctacggtctt
    21901 gcttccagtt tagtggcgag ggtggacgga tgacaacacc ataactggca gggtatgctt
    21961 aattccgcat tttccgatga gggataaaga tgagcaaacc acctcttttc tttattgtta
    22021 tcattggctt aatcatcgtc gccgcatcgt ttcgttttat gcaacaacga cgggaaaaag
    22081 ctgataatga tatggcacca ctccagcaaa agctggtgtt ggtgagcaac aagcgggaaa
    22141 aaccgattaa cgatcgccgt tcgcgccagc aggaagtgac tccggcaggc accagtatgc
    22201 gctatgaggc aagcttcaaa ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg
    22261 cccagcagta ccacgccctg acagtgggcg ataaaggtac gctgagctat aaaggaacgc
    22321 gctttgtcag ctttgtaggc gaacaataag gttatttttt aactttaaat ttcttctgcc
    22381 agaccagcag ttcaaacacg ccgaaaagga aaatccgtac cttttcggcg gtggtcatct
    22441 gcgggccatc tttcggtaaa gtggatttca atagcgccag ctgcatacca tgcatcagca
    22501 cggtaaaaat cagcgccacg ttaacgaaaa tattcagcgg acgtgggaag ggatgcacca
    22561 ggttgaggat taaaaatccc caaacacaga gcattaacaa acgaccaata ttaatcagca
    22621 tcactttctc cttgtgcttc gcgttgatac agccgataag ccacctgacc cgccactttt
    22681 tcccgatgta atgaccagtt tgctggaaca gtgggcagac cgttttcgac ttcgctttcg
    22741 acataaatca gggcttcgtc agccagccag ccgttatctt ccagtaaatt tatcgtctct
    22801 tctaacaagc cacggcggaa cggtggatcg acaaacacga tattatgcgg tgtgcctttt
    22861 tgcgccagga atgacattgc gttgctgttc accacgcgtg cattgcctgc ttttagtgtc
    22921 gccagattct taattaactg ctgagaaacc gcgcgatcca tctcaatcaa cgtggccccc
    22981 gcagcatagc gcgacaacgc ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga
    23041 cattgggcgt caacaatgac cggagccagc cagttaaaca acgtttcgcg tacgcggtcg
    23101 gtggtggggc gcagacctgg gctatcagga accgggagtt tacggcctcg ccactgcccg
    23161 ccaataatgc ggatttggcc gctgccggaa tgattcggtt ttttcatgat aaattgctca
    23221 atccgccaga taacacatac ttgcaggcgg tgatgtgaat taagttaagt gatagactat
    23281 ttcatcattt ttttagctgc tatgtacata gcgttaacgc tgtgccatga agcaacagcg
    23341 aggagtgtag tcgcaaatgg cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt
    23401 tggtcaaaaa gagcagaccc cggaaaaaga gacagaagtt cagaatgaac aaacggttgt
    23461 agaagaaatc gttcaggcgc aagagcctgt gaaggcttct gaacaagccg tcgaagagca
    23521 gccgcaggcg catactgagg ccgaggcgga aacttttgct gccgacgttg tggaagtcac
    23581 tgaacaggtt gctgaaagtg aaaaagcgca gcctgaagcg gaagtcgttg cacaaccgga
    23641 accggtcgta gaagaaacgc cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc
    23701 ggaagacgtc aacgccgaag aggtttcgcc agaagagtgg caggctgaag cggaaaccgt
    23761 agagattgtc gaagcggcgg aagaagaagc ggctaaagaa gaaatcaccg acgaagagct
    23821 ggaagcacag gcgctggctg ccgaagcggc agaagaagcg gtgatagtgg ttcctccggc
    23881 agaagaagag cagccggttg aagaaattgc ccaggagcag gaaaaaccga ccaaagaagg
    23941 tttcttcgcg cgcctgaaac gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt
    24001 tatcagcctg ttccgcggta aaaaaatcga cgatgatctg tttgaggagc tggaagaaca
    24061 gctgttgatc gccgatgtgg gcgtggaaac cacacgtaaa ataatcacca atctgacgga
    24121 aggcgcatcc cgcaagcagc ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga
    24181 gatgggcgag attctggcga aagtcgatga gccgctgaat gttgaaggta aaacgccgtt
    24241 cgtgatcctg atggtgggcg tcaacggtgt gggtaaaacc acgacgattg gtaagctggc
    24301 gcgtcagttt gagcagcagg gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc
    24361 agcagcggtt gaacagcttc aggtctgggg tcagcgcaac aatattccgg tgattgccca
    24421 gcataccggt gcggattccg cctctgttat cttcgacgcc attcaggcag ctaaagcgcg
    24481 taatatcgac gtcctgattg ccgataccgc cggacgcctg cagaacaaat cgcacctgat
    24541 ggaagagttg aagaaaatcg tccgcgtgat gaagaaactc gacgttgaag cgccgcatga
    24601 agttatgctg actattgatg ccagtaccgg gcagaatgcg gtaagccagg ccaaactgtt
    24661 ccacgaagct gttggcttaa ccggcatcac gctaacgaaa ctggacggca cggcgaaagg
    24721 cggggtaatt ttctcggtgg ctgaccagtt tggtatccct atccgctaca ttggtgtcgg
    24781 cgaacgtatt gaggatttgc gtccgtttaa ggcggacgac tttatagagg cactttttgc
    24841 ccgagaggat taacaatgat tcgctttgaa catgtcagca aggcttatct cggtgggaga
    24901 caggcgctgc agggcgttac gttccatatg cagccgggtg agatggcgtt tctgaccggt
    24961 cattccggcg cagggaaaag taccctcctg aagctgatct gtgggattga gcggcccagc
    25021 gccgggaaaa tctggtttag cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg
    25081 tttctgcgcc gccagattgg catgattttc caggatcacc atctactgat ggaccgtact
    25141 gtctacgata acgtggcgat cccgctgatt atcgctggtg ccagcggtga cgatattcgt
    25201 cgccgggtgt cggcggcact ggataaagtc gggctactgg acaaagcgaa gaacttccct
    25261 attcagcttt ccggcggtga acaacagcgt gttggcattg cccgcgcggt ggtgaataag
    25321 cccgcggtac tgctggcgga cgaaccgacc ggtaacctgg acgacgcgtt gtcggaaggg
    25381 attctacgtc tgtttgaaga gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac
    25441 gacatcaacc tgatttcacg gcgttcctat cgcatgctca ccctgagcga tggtcacttg
    25501 catggaggcg tgggccatga ataagcgcga tgcaatcaat catattcggc agtttggcgg
    25561 gcgtcttgat cgcttccgta aatcggtcgg cggctctggc gacggcggtc gtaacgcccc
    25621 gaaacgcgcg aaatcctcgc caaaaccggt aaatcgtaaa accaacgttt tcaacgaaca
    25681 ggtgcgctat gccttccacg gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt
    25741 tttaacggtg atggttatcg ccatctccct gacgctgccc agcgtctgtt atatggtgta
    25801 caaaaacgtt aatcaggcgg cgacgcagta ttatccgtca ccgcaaatca ctgtttactt
    25861 gcaaaaaacg ctggacgatg acgccgctgc gggcgtggtg gcacagttgc aggccgagca
    25921 aggcgtggag aaagtgaact atctttctcg tgaagacgca ctgggcgagt tccgtaactg
    25981 gtctggtttt ggtggtgcgc tggatatgct ggaagaaaac ccgcttccgg cagtggcggt
    26041 ggtgatcccg aaactcgatt ttcaggggac ggaatcactc aatacgctgc gtgatcgtat
    26101 cacgcagatt aacggcattg acgaagtgcg gatggatgac agctggtttg cccgtctggc
    26161 ggcgttgacc gggctggtcg ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc
    26221 agtgttcctc gtcatcggca acagcgtacg tctgagcatc tttgctcgcc gtgactccat
    26281 caacgtgcag aaactgattg gtgcgacgga tggtttcatc cttcgcccgt tcctgtatgg
    26341 tggcgcactg ctgggatttt ctggcgcatt gttgtcatta attttgtcag aaattctggt
    26401 gctgcgattg tcatcggcgg ttgcggaagt ggcacaggtt ttcggaacga agtttgatat
    26461 caatggttta tcgttcgatg aatgcctgct attgctgctg gtatgctcga tgattggctg
    26521 ggtggcagcg tggcttgcca cggtacaaca tttacgccac tttacgcctg aataataaaa
    26581 gcgtgttata ctctttccct gcaatgggtt ccgtagcagg gaaagagatc ccgttgtctc
    26641 ttcccggtat ttcatctcta tgtcacattt tgtgcgtaat ttattcacaa gcttgcattg
    26701 aacttgtgga taaaatcacg gtctgataaa acagtgaatg ataacctcgt tgctcttaag
    26761 ctctggcaca gttgttgcta ccactgaagc gccagaagat atcgattgag aggatttgaa
    26821 tgactgacaa aatgcaaagt ttagctttag ccccagttgg caacctggat tcctacatcc
    26881 gggcagctaa cgcgtggccg atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc
    26941 tgcattacca tggcgatctg gaagcagcta aaacgctgat cctgtctcac ctgcggtttg
    27001 ttgttcatat tgctcgtaat tatgcgggct atggcctgcc acaggcggat ttgattcagg
    27061 aaggtaacat cggcctgatg aaagcagtgc gccgtttcaa cccggaagtg ggtgtgcgcc
    27121 tggtctcctt cgccgttcac tggatcaaag cagagatcca cgaatacgtt ctgcgtaact
    27181 ggcgtatcgt caaagttgcg accaccaaag cgcagcgcaa actgttcttc aacctgcgta
    27241 aaaccaagca gcgtctgggc tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac
    27301 tgggcgtaac cagcaaagac gttcgtgaga tggaatcacg tatggcggca caggacatga
    27361 cctttgacct gtcttccgac gacgattccg acagccagcc gatggcaccg gtgctctatc
    27421 tgcaggataa atcatctaac tttgccgacg gcatcgaaga tgataactgg gaagagcagg
    27481 cggcaaaccg tctgaccgac gcgatgcagg gtctggacga acgcagccag gacatcatcc
    27541 gtgcgcgctg gctggacgaa gacaacaagt ccacgttgca ggaactggct gaccgttacg
    27601 gtgtttccgc cgagcgtgtg cgccagctgg aaaagaacgc gatgaaaaaa cttcgcgctg
    27661 ccattgaagc gtaatttcta ctactaagca gagaaccttg gatgagaatc cggggttttt
    27721 gttttttggg cctctgcaat aatcagtttc cccctccggc aaaacgccaa tccccacgca
    27781 gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatcggg tatgttttag
    27841 cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt gctgaacaac
    27901 aacatcacaa cacacgtaat aaccagaaga atggggaacc tcaggatgaa catgaagggt
    27961 aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct ggcagaagat
    28021 attaaagtcg cggtcgtggg cgcaatgtcc ggtccggtgg cccagtacgg tgaccaggag
    28081 tttactggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat taaaggcaac
    28141 aaactgcaaa tcgtgaaata tgacgatgcc tgtgacccaa aacaggcggt tgcggtggcg
    28201 aacaaagtcg ttaacgacgg cattaaatat gtgattggtc acctctgttc ttcatcaacg
    28261 cagccagcgt cagacatcta tgaagatgaa ggcattttaa tgatcacccc agcggcaacc
    28321 gcaccagagc tgaccgctcg tggctatcag ctgatcctgc gcaccaccgg cctggactcc
    28381 gaccaggggc cgacggcggc gaaatatatt cttgagaagg tgaaaccgca gcgtattgct
    28441 atcgtccacg acaaacagca gtacggcgaa ggtctggcgc gtgcggtcca ggacggcctg
    28501 aagaaaggca atgctaacgt ggtgttcttt gatggcatca ccgccgggga aaaagatttc
    28561 tctacgctgg tggcgcgtct gaaaaaagag aatatcgact tcgtttacta cggcggttat
    28621 cacccggaaa tggggcagat cctgcgtcag gcacgcgcgg cagggctgaa aactcagttt
    28681 atggggccgg aaggtgtggc taacgtttcg ctgtctaaca ttgcgggcga atcagcggaa
    28741 gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa acccattgtt
    28801 gacgcgatca aagcgaaaaa acaggaccca agcggcgcgt tcgtctggac cacctacgcc
    28861 gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga aatcgccaaa
    28921 tacctgaaag cgaactcggt tgataccgtg atggggccgc tgacgtggga tgagaaaggc
    28981 gatctgaaag gctttgagtt cggcgtattt gactggcacg ccaacggcac ggcaaccgat
    29041 gcgaagtaac agatagataa atgaaaatgc ccgatgcgct gaactgccat cgggtttttt
    29101 tgtgtttgtc gaaatggttg tagaaagatt aaacaatcca ggatatgctt aatgtacggt
    29161 gacataccgt acatattata cggagcagga gagttcacat gtctgctgtt aaaaagcagc
    29221 gtatcgatct gcgtttaact gacgatgaca aaagtatgat tgaggaagcg gcagcgatat
    29281 ctaatcagtc cgtcagccaa tttatgttga acagcgcttc gcagcgggct gcggaagtga
    29341 ttgaacagca tcggcgggtg atcctcaatg aggaatcctg gacgcgggtg atggatgcgc
    29401 tgagtaatcc accgtcacct ggtgaaaagc taaaacgtgc ggcaaaacgt cttcagggaa
    29461 tgtaataatt tatggatgat ctgacgatag agattctgac cgatgatgca gattatgatc
    29521 tacagcgatt cgactgcggc gaggaagcgt taaatctctt tctgacgaca catctcgttc
    29581 gtcaacatcg caacaaaatt ctgcgagcgt atatcctttg tcgcaacact ccagaacgtc
    29641 aggtgctggg atattacaca ttatgcggca gttgttttga acgagccgca ttgccctcga
    29701 aatcgaaaca gaaaaaaatt ccctacaaaa atattcccag cgttactctt gggcgtctgg
    29761 caattgatcg ttcattacag gggcagggat ggggagcaac actggttgct catgccatga
    29821 aagtcgtctg gtcagcctct ttagcggtag gtattcacgg tctttttgtc gaggcgctga
    29881 ataaaaaagc ccatacgttt tatcaatcgc tgggctttat cccgttggtc ggagaaaacg
    29941 aaaatgcgtt atttttccca accaaatcca tcgaactgct tttcacacag agcgattaac
    30001 gcttctccca gccgccctgt tgcgccgtaa atcccagtgc ctgcataaac gccgtcatca
    30061 caccgcgatc ttccacgcct gcatccgcca tccaccagca tgaaacgcca ggattgttac
    30121 gcaaaacctc ttccagcaga tattgcccca caccgcgacg gcgggtgact tcccgcacgc
    30181 acagggaatc cagtgctccc tcggtgccgc ttaaagttac ccgcacggcc gctagcaggc
    30241 gctcgttaaa acgcgcggcg tagatacggt ggttatcgtc aacctgtaaa gaggaagggg
    30301 aatactccgg ccagatcttt tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc
    30361 gaatgatggt cagcttcatg gggaacccgt gtaaatcaca aaagtggaac cagtgtagcg
    30421 aaataattta atcggaggct ttctcttttt tatttctttt ggcag
//
LOCUS       DAMZII010000049        30092 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZII010000049 DAMZII010000000
VERSION     DAMZII010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.049"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            82..3816
                     /gene="yfaL"
                     /locus_tag="POA77_002155"
     CDS             82..3816
                     /gene="yfaL"
                     /locus_tag="POA77_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDI6365593.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSVSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHNPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(3944..4666)
                     /locus_tag="POA77_002156"
     CDS             complement(3944..4666)
                     /locus_tag="POA77_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDI6365594.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4813..7440
                     /gene="gyrA"
                     /locus_tag="POA77_002157"
     CDS             4813..7440
                     /gene="gyrA"
                     /locus_tag="POA77_002157"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDI6365595.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7589..9277
                     /locus_tag="POA77_002158"
     CDS             7589..9277
                     /locus_tag="POA77_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDI6365596.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQAQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9274..9897
                     /locus_tag="POA77_002159"
     CDS             9274..9897
                     /locus_tag="POA77_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDI6365597.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9831..14435
                     /locus_tag="POA77_002160"
     CDS             9831..14435
                     /locus_tag="POA77_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDI6365598.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLANADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVNVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKDARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGV
                     VDEMIYALQPEIAPNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSER
                     RVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQ
                     GRAYLRSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLT
                     LHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGG
                     ENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPWGGVINTGSRLIPLSLAWRSLA
                     DHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQA
                     LGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRG
                     TKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAW
                     DKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQSTIERALAMNWLAKYMATMPP
                     VVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKMAQQS
                     NIPVTVERQLYRLIPGEEEMSFILQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVE
                     VPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRH
                     LLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            14436..16085
                     /locus_tag="POA77_002161"
     CDS             14436..16085
                     /locus_tag="POA77_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDI6365599.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16090..16866
                     /locus_tag="POA77_002162"
     CDS             16090..16866
                     /locus_tag="POA77_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDI6365600.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17141..19990
                     /gene="rcsC"
                     /locus_tag="POA77_002163"
     CDS             17141..19990
                     /gene="rcsC"
                     /locus_tag="POA77_002163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDI6365601.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20190..20840)
                     /gene="rcsB"
                     /locus_tag="POA77_002164"
     CDS             complement(20190..20840)
                     /gene="rcsB"
                     /locus_tag="POA77_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDI6365602.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20857..23529)
                     /gene="rcsD"
                     /locus_tag="POA77_002165"
     CDS             complement(20857..23529)
                     /gene="rcsD"
                     /locus_tag="POA77_002165"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDI6365603.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24268..25371
                     /gene="ompC"
                     /locus_tag="POA77_002166"
     CDS             24268..25371
                     /gene="ompC"
                     /locus_tag="POA77_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6365604.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25483..26538
                     /gene="apbE"
                     /locus_tag="POA77_002167"
     CDS             25483..26538
                     /gene="apbE"
                     /locus_tag="POA77_002167"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDI6365605.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLVVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            26612..27676
                     /gene="ada"
                     /locus_tag="POA77_002168"
     CDS             26612..27676
                     /gene="ada"
                     /locus_tag="POA77_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDI6365606.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27676..28326
                     /gene="alkB"
                     /locus_tag="POA77_002169"
     CDS             27676..28326
                     /gene="alkB"
                     /locus_tag="POA77_002169"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDI6365607.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28402..30045
                     /gene="yojI"
                     /locus_tag="POA77_002170"
     CDS             28402..30045
                     /gene="yojI"
                     /locus_tag="POA77_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDI6365608.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         7305 a         7364 c         8192 g         7231 t
ORIGIN      
        1 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
       61 taaaatgatg gttagatgaa tatgcggatt atctttctac gcaaggagta tttatctcta
      121 ctcccgtcaa tgattgcatc tcttttctct gctaacggtt tcgcggcggc cattgattta
      181 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
      241 acgcaggact ggagtgtcgc cgatgggcaa tggctggttt tttcggatat gaccaataac
      301 gccagcggag gagccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat
      361 gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg
      421 gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttctggtaac
      481 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaatgatac tggtgccgtc
      541 gatttacgtg tcactaacgc catgtttcgc aataatatcg ctaatgatgg caaaggtggc
      601 gcaatttata ccattaataa tgacgtctat ttaagtgatg ttatttttga taacaaccag
      661 gcatatacat caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat
      721 aatagcgtca gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat
      781 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
      841 attgatattt ctgttgatga cagctacagc cagaacggcg gcgtgttagt ggatgagaac
      901 aacagcgcag caggctatgg agatggtcct tccactgcgg cgggtggctt tatgtatctc
      961 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
     1021 gagaatgacg gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc
     1081 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
     1141 ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
     1201 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
     1261 gcagagctaa acgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag
     1321 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
     1381 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctgtcg
     1441 ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg
     1501 ctggaaggcg acgcggcaga tctcgccgct ctccaggacg atccgcagtc gatcgtgtta
     1561 aacggcggtg tgctcgatct ctctgatttc tccacctggc agagcggcac atcatacaat
     1621 gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
     1681 cttgcaggtg gcgacaattt gcatatcggc ggcgacggga aagatggcgt ctacgtggtg
     1741 gtcgatgcga gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca
     1801 caaatcgcct ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccactat
     1861 aaccgccagg ttatctttac cgataagcaa caagaaagcg tgatggagat tactgccaat
     1921 gtcgatactc gctctacgac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
     1981 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
     2041 gggcagcatc aggatgaggg tagcacattg actaaaacgg gggcgggtac gctggagctg
     2101 accgccagcg gtacaacgca gtcggcagta cgtgtcgaag aaggcaccct gaaaggcgat
     2161 gttgcggata tctttcctta tgcttcgtca ctgtgggtcg gtgatggggc gacgttcgtt
     2221 actggcgcgg atcaggatat tcagtctatc gatgctactt ccagcggcac tatcgatatc
     2281 agcgatggta cggttttgcg tctgaccggg caggatactt ccgtcgccct taatgcctca
     2341 ttgtttaacg gcgatgggac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag
     2401 cttaatacca accttgaaac tgacagcctg acctatcttt ccgacgtgac ggttaatggc
     2461 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
     2521 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
     2581 gatgactcgg taagcgatca attggtgttg aacggtaata ctgctggcaa cacaactgtg
     2641 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
     2701 ttcgcagccg atcccacgca gtttcaaaac aatgcgaagt tcagtctggc aggcagcggc
     2761 tacgtcaata tgggagcata tgactacacg ctggtggaag ataacaacga ctggtatctg
     2821 cgatcgcaag aagtaacgcc accattgcca cctgatccag accctgaccc aacacccgat
     2881 cctgacccaa cacccgatcc aatccccgct taccagccgg tgctgaatgc caaagttggc
     2941 ggttatctca ataatctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcat
     3001 gcaggtggca atggtcagac gctgaattta cgtgttatcg gcggacgtta tcattacacg
     3061 gcggcagggc aactggctca gcatgaagac acttctacgg tgcagcttag cggtgacctg
     3121 tttagcgggc gctggggcga tgatggcgag tggatgcttg gggcggttgg tggctacagt
     3181 gataatcagg gcgagagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac
     3241 ggttatgccg ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa gcagggggcc
     3301 tggctggata gctggctgca atacgcgtgg tttaacaatg atgtttccga acaagacgat
     3361 ggcacagatc attaccactc gtcggggatt atcgcctcgc tggaagccgg gtatcagtgg
     3421 ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag
     3481 caggatgatt ttaccgccgc taaccgtgcg cgcgtgtcac aatcgcaggg tgatgatatt
     3541 cagatgcggc tgggtttaca cagcgaatgg cgtaccgctg ttcatgtcat accaacatta
     3601 gatctgaatt attatcacaa tccccattcg acggaaattg aagaggatgg cagcactatc
     3661 agtgacgatg cggtgaagca acggggtgaa ataaaagtgg gagtcacggg caatatcagt
     3721 cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag
     3781 atggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg gcacggttgc
     3841 cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc tgataagacg
     3901 cggcaagcgt cgcatcaggc attggctcgg ccaaacaaca acctcactta ttctgcgtgt
     3961 gcagcatata gttcacatcc acgccggggc cgagtttaaa agtattagtg agcgggttgt
     4021 aatgtagccc ggttatatgt cgctctttaa ggcttgtctg atctacccag ccaagcagtt
     4081 ctgccggttt aataaacttc ttcacatcat gcgtgccttt gggcaccatg cgcaaaatat
     4141 attccgcgcc aaccaccgcc atcagccatg acttgccgtt gcggttaagc gtcgagaaaa
     4201 agacatcgcc gcctggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg
     4261 ggacgtgctc cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg
     4321 cgtgctcttc cacggtttcc tgcacataat ccacctgaat gccgctttcc agcgcgtgca
     4381 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc
     4441 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
     4501 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcaacg
     4561 gtttgaactc accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
     4621 cttcgtggtc tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc
     4681 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
     4741 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
     4801 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
     4861 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
     4921 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
     4981 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
     5041 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
     5101 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
     5161 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
     5221 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc
     5281 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
     5341 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
     5401 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
     5461 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
     5521 cgcggcaagg tgtatatccg cgctcgcgca gaagtggaag ttgacgccaa aaccggtcgt
     5581 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
     5641 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
     5701 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
     5761 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
     5821 ttgcaccatg gtcagccgaa gatcatgaac ttgaaagaca tcatcgcggc gtttgttcgt
     5881 caccgccgtg aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
     5941 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
     6001 atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgc tggttgctaa tccgtggcag
     6061 ctgggcaacg ttgccgcgat gctcgaacgt gctggcgacg atgctgcgcg tccggaatgg
     6121 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
     6181 gcgattctgg atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac
     6241 gaatacaaag agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat
     6301 cgtttgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
     6361 cgtcgtactg aaatcaccgc caacagcgca gacattaacc tggaagatct gatcactcag
     6421 gaagatgtgg tagtgacgct ctctcaccag ggctacgtta agtatcagcc gctttctgaa
     6481 tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
     6541 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
     6601 ggtcgcgtct attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt
     6661 cgtccgatcg tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca
     6721 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag
     6781 aaaactgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
     6841 gttgacggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
     6901 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
     6961 aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc
     7021 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc
     7081 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc cattaaggtt
     7141 actgaacgta atggtttagt ggttggcgcg gtacaggtag atgactgcga ccagatcatg
     7201 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtaggc
     7261 cgtaacaccc agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg
     7321 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgatgg cagtgccgcg
     7381 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
     7441 ttttacttct tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct
     7501 gcaaagtctg ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa
     7561 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
     7621 gttctatggt cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt
     7681 gcaatatgct ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct
     7741 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
     7801 caaagatctc ctcaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
     7861 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgtcgta ttgtctatga
     7921 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc
     7981 agcgctgtgg cacgataaac aaggccatct ttcacattat atggtgctga tccagcgcag
     8041 tggtttaagc aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag
     8101 caaaacggaa atcagtagca tcaagataaa tagtgaaact gttcctgttt atcagttgcg
     8161 ctataacggc aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc
     8221 cagcacggat atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg
     8281 tgatttgttg agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc
     8341 tgaaaaaacg ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta
     8401 ccagcggtta atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca
     8461 cagttttgtg gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc
     8521 ggtctggaac agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg
     8581 tattgccgaa gagatgcttt cgcacatcag tcaggaaaac gacaagttga atggggcgtt
     8641 agacggtgcc gcggggctgt gctggtatgc agactcaaaa ttgcaaaccc cgctgtttgt
     8701 cggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
     8761 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccgataagcc aggctcagca
     8821 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc
     8881 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat
     8941 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac
     9001 actgaataaa aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
     9061 ctatatcaat ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc
     9121 gaagaatctc gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
     9181 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
     9241 ctggcagtgg ctacccataa cctggcagcc gctatgaggc acgggctgct ggcgctgatt
     9301 tgctggctgt gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg
     9361 tttcgcgcct ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc
     9421 tggtatcagc aggattgtgc gggcctggtg cgatttgcgg cgaacgagac gctgaaagtt
     9481 cacgatagta aatggctaaa aagtaacggt ttctctagcc agtatttgcc gccagagatg
     9541 acgctaacac ctggacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc
     9601 ggcccctacg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
     9661 ataaaccagg cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
     9721 atggtctgga tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac
     9781 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc
     9841 aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag
     9901 cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt
     9961 ccagtaacta tgcgccgccc gccgggggaa cattcttttt gcttgctgac agcagtttta
    10021 gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc
    10081 agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat
    10141 ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ctgggcgacg
    10201 ggctgaacaa tacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtaa
    10261 tgcagcgtac tttctcttct cagtcacggc agaatgtgac tcaggcatta cccgaattac
    10321 atctcggcaa tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttccccgc
    10381 tgaaaaaata tcccttggtg gaacagttcc gttatccact atggcaggct aaaccggtcg
    10441 agccgcagca aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg
    10501 gtaacattta tattcctctc ggccaacaag agccgggact gtacctcgtc gaggcgatgg
    10561 ttggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag
    10621 tgtcaggcaa cgagcttctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg
    10681 gctcagagat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca
    10741 gcggtacctt gcagttacaa catatatcgc cagaacgttc atacattctg ggtaaggatg
    10801 ctgaaggcgg tgtttttgtc tccgagaact tcttctacga aagcgaaatc tacaacaccc
    10861 gcttgtatat ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag
    10921 tgatgggccg cgagttccac gatccgttgc attcatcccc catcgtcagc gccccggcga
    10981 agctttcggt gctggacgct aacggcagtc tgttgcaaac cgtcaatgtc acgctggatg
    11041 cgcgcaatgg cgggcaggga agtttccgcc tgccagaaaa tgccgtagcc ggaggttatg
    11101 agttacgtct tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg gcaaactaca
    11161 tcaagccaca tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa actggcgaag
    11221 cggtcagcgg caaactgcaa ctgctttacc cggatggtga gccggtaaaa gatgcccgcg
    11281 tgcagttaag tttgcgcgct cagcaattat caatggtcgg taacgatttg cgttatgccg
    11341 gacgttttcc cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagc ggtcatgtgg
    11401 cgttaaatct ccccgccgcc gataaaccga gccgctattt gttaaccgtc tccgccagtg
    11461 acggcgcggc gtatcgcgtc accaccacca aagagatcct cattgaacgc ggcctggcgc
    11521 attactcttt aagtaccgcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt
    11581 atgccgcgct ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag
    11641 accgcacgag ccatagcgga gagctaccgt caggcggcaa atcctttacc gtcaatttcg
    11701 ctaaacctgg caactacaat ctgacattac gcgataaaga cggcttaatt ctcgctgggt
    11761 taagtcatgc cgtcagcggt aagggcagca cggcgcatac tggtacggta gatatcgtgg
    11821 cggataaaac gctgtaccag ccaggcgaaa ctgcgaagat gctgattacc tttccggagc
    11881 caattgatga agcattattg acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt
    11941 cgcatccggc aaactggctg acgttacaac gtttaaacga tacccagtat gaagcccggg
    12001 ttccagtgag caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaatg
    12061 gtcagtacag ttttcagaac gccgggatca aagttgccgt tcctcagctt gatatccggg
    12121 tgaaaacgga caaaacccat taccagcctg gtgaactggt caatgtcgaa ttaacctcgt
    12181 cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat gaaatgatct
    12241 acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta
    12301 acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg
    12361 agccggttgc gcctggcgca actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc
    12421 cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag
    12481 gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc
    12541 gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa
    12601 atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa ccggcggcag
    12661 gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag
    12721 gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctgacgc
    12781 agaacattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg caagtgcagg
    12841 acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa cagcagaaaa
    12901 atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg agtaatatcc
    12961 ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg
    13021 atggaccatg gggtggcgtg atcaacaccg gtagccgtct gatcccgctc agtctcgcct
    13081 ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag atgattcagg
    13141 ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc tggtggggtg
    13201 aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac tggcaggcca
    13261 gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc gacagttatg
    13321 ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg tgggcacagg
    13381 agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc gcccggcgcg
    13441 gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac agcctgatcc
    13501 tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc atgacgttgc
    13561 tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat gcgtgggata
    13621 aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat agcggtggtg
    13681 acgctaccca gacggccgct attttaagtg gtttgaccgc tgagcaatcc actattgagc
    13741 gcgcgctggc catgaactgg ctggcgaaat atatggcgac gatgcctcca gttgttttgc
    13801 ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa gactggcgtt
    13861 gggttggtca gggcgtgccg gacattctct cttttggtga cgaattatcg ccgcaaaatg
    13921 tgcaggtccg ctggcgtgag ccggcaaaaa tggctcaaca aagtaacatt ccggtgaccg
    13981 ttgaacgcca gttgtatcgg cttatccctg gtgaagaaga gatgagcttt attctgcaac
    14041 cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt acgcttacca
    14101 gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg ccgggagccg
    14161 acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg aaacagcagg
    14221 ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg gtgccggtga
    14281 aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa aaagggcaat
    14341 tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa agtgttgcgg
    14401 cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga atcgtctggc
    14461 tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa ctggtgctgc
    14521 gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc aaagcctcga
    14581 cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat gtttacgcct
    14641 ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac tccccggaag
    14701 aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg gtacgttcct
    14761 gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg gggcaatact
    14821 ggcaacagcg tcaggcacct gcgtggttag cgtctcttac tacgctgaag ccagaaacct
    14881 cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa aacaaagctc
    14941 aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggagtcgca tccatgctcg
    15001 gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag gaaatccgtc
    15061 agggtggttt tgccggctgg ttaaccgatg gtacaccgct gtgggctact ggcagtggca
    15121 ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg ccagtgccga
    15181 cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga
    15241 aaatcacagc ggaaaagagt acgacggcag taaaacccgg cgtacttaat gggcgctatc
    15301 gtgtgacgtt taccaatggt aaccacatta cgtttgtcag ccacggtgag acgaccttac
    15361 tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag tacgttgcca
    15421 gagttctgga tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg
    15481 cgattcgcac ttttttacag caaaacgcca atcgggaagg cgattgcctg accatccccg
    15541 acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg cgaacgatga
    15601 ccgcctggac gcaggatctc atctatgcag gcgatccggt tcattatcat ggcagtcgcg
    15661 ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag ggggagcgtt
    15721 acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg ggagctccac
    15781 gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa atgccgcagt
    15841 ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt gcggtctgtc
    15901 gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt attcgtaact
    15961 tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg gcctttgatg
    16021 gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa ttattaatgg
    16081 actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc gcctgtcgct
    16141 cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg gcatccggca
    16201 gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg
    16261 gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg
    16321 ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca gagagttgaa
    16381 tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag cgtgcaggtc
    16441 atcagcccgg atgggcaaag ccgacaaaaa atgcagtttt actcaacgcc gggtacagga
    16501 acgattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac cgatctcgat
    16561 ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt gctgaaaaac
    16621 agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt cgccatgcca
    16681 gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg cagcgaaacg
    16741 gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa tgagaaacag
    16801 gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa aagttttgac
    16861 tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga ctgctgggcg
    16921 atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca tttcttattt
    16981 ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg gtttctaagc
    17041 gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac cgctacctta
    17101 accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc ttgcttcttt
    17161 tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag tgctctggct
    17221 gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc gagaatcgga
    17281 aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc aacgcacctc
    17341 tgatgtgatg aaagagctga agtacatcgc cgaaaatcgc ttatcggcag aaaacggtgt
    17401 gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac cgctgtttgc
    17461 cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt cattggcgtg
    17521 gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc gggtattttt
    17581 aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc cagtggaacg
    17641 cgataccgcg ttgaaagctt tgcatgaacg catcaataaa tatcgaaatg caccacaaga
    17701 tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg tcgggtattt
    17761 ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg gtgtcgagca
    17821 gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg ttaccattct
    17881 tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa ttaagggcga
    17941 tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc gggagctggt
    18001 gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc cggttgataa
    18061 ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg tgcttgccgg
    18121 agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc cggcggaaag
    18181 cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg cctccgcgcc
    18241 agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta acgaactggc
    18301 gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc aaattatctg
    18361 tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc tgcaaatcag
    18421 cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg tggatgtttc
    18481 ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg aacaggcgag
    18541 ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc cgctgtatgg
    18601 cattatcggt aaccttgatc tgttgcaaac caaagagtta ccgaaaggcg tcgatcggct
    18661 ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg atattctcga
    18721 tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt caccgcgtga
    18781 agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc agttaggctt
    18841 gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga tgcgtttaca
    18901 gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg gctgtatagt
    18961 tttgcatgtc cgcgctgatg gcgattatct ctctatccgt gttcgcgata ccggcgtggg
    19021 gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg gaacgggcgt
    19081 acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac tgatcagcat
    19141 gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt ttaccgtgcg
    19201 tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt tgagtggtaa
    19261 acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa ccagtttgca
    19321 gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg aagatgtgtt
    19381 aattactgac gaggtagtga gtaaaaaatg gcagggtaga gcggtagtga ccttctgtcg
    19441 tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca gtgtggctgc
    19501 cccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg agagcgacga
    19561 tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataatgacg atatgatgat
    19621 tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt tgggatcgtt
    19681 gggctatcaa tgtaaaaccg cgaatgatgg tgtcgatgcg cttaatgtac ttagcaagaa
    19741 tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt accgcttgac
    19801 gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg ctaatgcgtt
    19861 ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctgt cgaagccggt
    19921 aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca ggaaatcgcg
    19981 ggaatcgtag gacggataag gtgtttatgc cgcatctggc attcagtaca aatgccagat
    20041 gcgacgctga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat aatgggaatc
    20101 gtaggccgga taaggcgttt acgctgcatc cggcaatcgg tgcaaatgcc agataagaca
    20161 ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact taaggtcaca
    20221 gaagagagat aattcagcag ggcgatatcg ttctcgacac ccagcttcat catcgcagat
    20281 ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat ctcggtcacc
    20341 aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag acgcttgtca
    20401 ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg ggtaaatttc
    20461 ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc ttgtttcagc
    20521 acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt gttgttcatg
    20581 gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat taaggtaatg
    20641 ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg cgcatccagt
    20701 ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac attcacccac
    20761 tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc atcggcaata
    20821 attacgttca tattgttcat gtaataggct accttgctac agcaagctct tgacataact
    20881 gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa tcagatgttc
    20941 cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata cgcctttaag
    21001 acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag tatacagcct
    21061 cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg catggagttg
    21121 cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca gttgcacttc
    21181 aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga cgcacaattg
    21241 accaggccca atttcccgta cgccagactc atcatcgctt aaaagcaagc cagaggcagt
    21301 aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta atctttcatc
    21361 gggtgtgata caggttgcac cccaattttc taactggcga gtgacaatat tccgaatttc
    21421 tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt cttcctcttc
    21481 aacttccggg tcagctgcga gcattttgat atgcacagag tagcgtgtac caagcccatc
    21541 ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc tcagccagaa
    21601 tgccagcggg tccgccttgc catagcgatc gttttgggtc tggttgataa acgggaagtg
    21661 caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc ggaacgtcag
    21721 gcggtcttcg gaggactcat cctgatcaac ctcaagggtg atttttccca attgcgttga
    21781 ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc gatcgccgcg
    21841 gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt tacgcttgat
    21901 ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga acagcaccgt
    21961 ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttcat caaccagccg
    22021 caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga gtttagccgc
    22081 gctctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt
    22141 catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt tcttgtttac
    22201 cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg cgacctggct
    22261 gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa tcccctgatg
    22321 ctgttccgcc atggtggtga tgttttgcag attcaaatgc ggcagcaaat gatcggcaat
    22381 tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc cgagcggcag
    22441 cagtgagact atctcttcat taattgcccg taaaatgcgc aattcgttat tgaccgccgt
    22501 gctgggtaga ctttctgtac tgcggctgga gaaatggcgg aatgtggtat agccaaataa
    22561 cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt gcaacgtatc
    22621 cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag agttgagtgc
    22681 cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg tcccttcttt
    22741 ctcattatca ttgtctcccg ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg
    22801 catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg ttgccagatg
    22861 tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg
    22921 gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca acatttctgc
    22981 acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg tgagatcttt
    23041 gaggggcagg gttgagatca gcaccagact gttatcctga ccattcaggt aatacatcga
    23101 ccacggtaca ttttctgcgc cccacaatgt gtccagatag gtggacatcc gttgagtcat
    23161 ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc ggcgcggttt
    23221 ttccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg
    23281 ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac
    23341 gcgcttttgg atagcgtggg taatgtcgac aatctggtaa cttttatctt tcagccaggc
    23401 gttaacggcg ctttgtacca ttacccccat cgtcaccagt aacacaatga tcaacagtaa
    23461 aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt
    23521 ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc agtataaagg
    23581 gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc ccgtgatatt
    23641 tatcatcctg cattggggga aatagatgta caagcgccat tttgctgtac aaataaccta
    23701 caaaaagccc aacaaaaata attgcaaata aacaaagatt gctggaaatt atgctgatgt
    23761 taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca cagaataatg
    23821 aaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaataggggt
    23881 aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat agcgggagtt
    23941 attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata cttaagaata
    24001 agttattgat tttaaccttg aattattatt gcttgatgtt aggtgcttat ttcgccattc
    24061 cgcaataatc ttaaaaagtt cccttgcatt tacattttga aacatctata gcgataaatg
    24121 aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt tttggggaga
    24181 atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa aaaagcaaat
    24241 aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct cctggtccca
    24301 gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga cggcaacaaa
    24361 ttagatctgt acggtaaagt agacggcctg cactatttct ctgacaacaa agatgtagat
    24421 ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac tgaccagctg
    24481 accggttacg gccagtggga atatcagatt cagggcaaca gcgctgaaaa cgaaaacaac
    24541 tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtaggttc tttcgactac
    24601 ggtcgtaact acggcgttgt ttacgacgta acttcctgga ccgacgtact gccagaattc
    24661 ggtggcgaca cctacggttc tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc
    24721 taccgtaaca ctgacttctt cggtctggtt gacggcctga actttgctgt tcagtaccag
    24781 ggtaaaaacg gtagcgtaag cggcgaaggc atgaccaaca acggtcgtgg tgctctgcgt
    24841 cagaacggcg acggcgttgg cggatctatc acttatgatt acgaaggctt cggtatcggt
    24901 ggtgcgatct ccagctccaa acgtactgat gatcagaaca gcccgctgta catcggtaac
    24961 ggcgaccgtg ctgaaaccta caccggtggt ctgaaatacg acgctaacaa catctacctg
    25021 gctgctcagt acacccagac ctataacgca actcgcgtag gttccctggg ttgggcgaac
    25081 aaagcacaga actttgaagc tgttgctcag taccagttcg acttcggtct gcgtccgtct
    25141 gtagcatacc tgcagtctaa aggtaaaaac ctgggtacca tcgctggtcg taactacgac
    25201 gacgaagata tcctgaaata tgttgatgtt ggtgctacct actacttcaa caaaaacatg
    25261 tccacctacg ttgactacaa aatcaacctg ctggacgaca accagttcac tcgtgacgct
    25321 ggcatcaaca ctgataacat cgtagctctg ggtctggttt accagttcta atctcgattg
    25381 atatcgaaca aagggcctgc gggccctttt ttcattgttt tcagcgtaca aacacagttt
    25441 tttggtgtac tcttgcgacc gttcgcatga ggataatcac gtatggaaat aagctttacc
    25501 cgcgtggcac tgctggctgc cgcgctcttt tttgttggtt gcgatcaaaa accacaaccc
    25561 gccaaaaccc acgctactga agttaccgtt cttgaaggca aaaccatggg taccttctgg
    25621 cgtgccagca tcccgggcat tgatgccaaa cgcagtgccg aacttaaaga aaagattcag
    25681 acccagctgg acgccgacga tcagctgctt tcgacctata aaaaagattc cgcgctgatg
    25741 cgctttaacg attcgcaaag tttatcgccg tggccggtaa gtgaagcgat ggccgatatc
    25801 gtcaccacct cgctgcgcat tggcgcgaag accgatggcg cgatggatat aaccgtcggg
    25861 ccgctggtga atctgtgggg ctttggcccg gaacaacagc cggttcaaat tccgagccag
    25921 gaacagatcg atgcgatgaa agccaaaacc ggcttacagc acctgacggt cattaatcag
    25981 tcgcatcagc aatatctgca aaaagacctg ccggatttaa atgtcgatct ctctaccgtc
    26041 ggcgaaggtt atgcggcgga tcacttggca cgcttgatgg agcaggaagg gatttcccgc
    26101 tatctggtgt cggtgggcgg cgcgctgaac agccgtggta tgaacggtga aggccagccg
    26161 tggcgggtag cgattcaaaa accaaccgat aaagaaaacg cggttcaggc tgtggtggat
    26221 atcaacggtc atgggattag cacctctggc agctaccgta actattacga actggacggc
    26281 aaacgtcttt cccatgttat cgatccgcaa accgggcgtc ccatcgaaca caatctggta
    26341 tccgtgacgg tgattgctcc gacggcgctg gaagccgatg cctgggatac tggcttgatg
    26401 gtactcgggc cggagaaagc caaagaggtt gttcgccggg aagggctggt ggtttatatg
    26461 atcaccaaag aaggcgatag ctttaaaacc tggatgtcac cacagtttaa aagcttcctt
    26521 gtcagcgaaa aaaattaaag cgcaagattg ttggtttttg cgtgatggtg accgggcaga
    26581 ctaaaggcta tccttaacca gggagctgat tatgaaaaaa gccacatgct taactgacga
    26641 tcaacgctgg caatctgtct tagcccgcga cccgaatgcc gacggcgaat tcgttttcgc
    26701 cgtgcgtacc acaggcatct tttgccgtcc gtcttgccgc gccagacatg ctttgcggga
    26761 aaacgtctcc ttctacgcaa atgccagcga ggcactcgcc gctggctttc gcccctgcaa
    26821 acgttgtcag ccagaaaaag ccaatgccca gcaacatcgg ttggataaaa tcacccacgc
    26881 gtgtcgactg ctggaacagg aaacgcctgt aacgttggaa gccttagccg accaggtggc
    26941 gatgagtcca tttcatctac atcggttgtt taaagcgact accggaatga cgcctaaagc
    27001 ctggcaacag gcctggcgcg ctcgccgttt gcgcgaatcg ctggcgaaag gggagagcgt
    27061 gacgacgtct attcttaacg ccggattccc cgacagcagc agttactatc gcaaagctga
    27121 cgaaacgctg ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg
    27181 ttacgcgctg gctgattgtg agctgggtcg ttgcctggtg gcagaaagcg agcgggggat
    27241 ttgcgcgata ttgctgggcg atgatgacgc gacactaatc agcgagttgc agcagatgtt
    27301 tcccgctgcc gacaacgcgc ctgccgatct gatgtttcag caacatgtgc gtgaagtgat
    27361 cgccagcctc aatcaacgcg atacgccgct gacgttaccg ctggacattc gcggcactgc
    27421 ttttcagcaa caagtctggc aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta
    27481 tcagcaactg gctaacgcca tcggcaaacc gaaagcggta cgggccgttg ccagcgcctg
    27541 tgccgccaac aagctggcta tcataatacc ctgtcatcgg gtggtccgtg gtgatggcac
    27601 actttccggt taccgctggg gcgtgtcgcg taaagcgcaa ctgctgcgcc gcgaagctga
    27661 aaatgaggag aggtaatgtt ggatctgttt gccgatgctg aaccgtggca agagccactg
    27721 gcggctggag cggtaatttt gcaccgtttt gcttttaacg ctgcggagca actaatccgc
    27781 gatattaatg acgttgccag ccagtcgccg tttcgccaga tggtcacccc cgggggatat
    27841 accatgtcgg tggcgatgac caactgtggg catctgggct ggacgagcca tcggcaaggt
    27901 tatctctatt cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccacagagt
    27961 tttcataatt tatgtcaacg tgcggctacg gcggcgggct atccagattt ccagccagat
    28021 gcttgtctta tcaaccgcta cgctcctggc gcgaaactgt cgctgcatca ggataaagac
    28081 gaaccggatc tgcgcgcgcc aattgtttct gtttctctgg gcttacccgc gatttttcaa
    28141 tttggtggcc tgaaacgtaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg
    28201 gtggtatggg gcggtgaatc gcggctgttt tatcacggta ttcaaccgtt gaaagcgggg
    28261 tttcatccac tcaccaccga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa
    28321 gaataaaaat aagaattatt attgctgtgc gcgcgaagat gtttaaactg cgggctgtaa
    28381 ttcattgtcc gggttttctg catggaactt cttgtacttg tctggcggca gtatcgctgg
    28441 ccatttatca gtgtgatggc gctaagcctc gccagtgcgg cattaggcat tggcttaatt
    28501 gcttttatca atcagcgcct tatcgaaacg gcggatacca gtctgctggt attgccggag
    28561 tttctgggat tattgctgct gttgatggca gtcactctcg gatcgcaact ggcgctcacc
    28621 actttggggc atcacttcgt ttaccgactg cgtagtgaat ttatcaagcg gattctggat
    28681 accaacgtcg agcgcattga acaactcggt agcgcctcgt tgctggcggg attaaccagc
    28741 gatgtgcgca atatcaccat tgcttttgtg cgtcttccgg aactggtgca ggggatcatt
    28801 ctcactatcg gttccgcggc atatctgtgg atgctgtcgg gcaaaatgtt gctggtaacg
    28861 gctatctgga tggcgatcac catctggggt ggttttgtac tggtggcgcg ggtgtacaaa
    28921 catatggcga ccctgcgtga aaccgaagac aagctgtata cggattttca aactgtactc
    28981 gaagggcgca aagagctgac tctgaaccgg gaacgcgccg agtatgtgtt taacaacctc
    29041 tacattcctg atgcgcaaga gtatcgccac catattatcc gcgcagacac cttccatctt
    29101 agcgccgtga actggtcaaa catcatgatg ttgggcgcaa tcggcctggt gttctggatg
    29161 gcgaacagtc tcggttgggc tgataccaac gttgccgcga cctattcgtt gacgctttta
    29221 ttcctgcgta cgccgctgct ttcggcggtt ggcgcattgc cgacgctgct gacggcgcag
    29281 gtggcgttta acaagctgaa caaattcgcg ctcgcgcctt tcaaagcaga gtttccgcgc
    29341 ccgcaggcgt ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt tgcttatcag
    29401 gataacgcgt tttccgttgg tccgattaat ctcaccatca aacgtggcga gctgctgttt
    29461 ctgattggtg gcaacggtag cggaaaatcg acgctggcga tgttgttgac gggcttgtat
    29521 cagccacaaa gcggcgaaat cttgctggat ggcaaacctg tcagcggcga acaaccggaa
    29581 gattatcgca aactgttttc ggcagtgttt accgatgtct ggctgtttga tcaactgctg
    29641 gggccggagg gtaaacccgc taacccgcaa ctggttgaga agtggctggc gcagctgaaa
    29701 atggctcata agcttgagtt aagcaacggg cgtattgtta acctgaagtt atcaaaaggg
    29761 cagaaaaaac gcgtggcgct gttgctggcg ctggcagaag aacgcgatat tatcctgctg
    29821 gatgaatggg cggcggatca ggatccacac ttccgtcgtg agttttatca ggtgttgctg
    29881 ccgctgatgc aggagatggg taaaactatt ttcgccatca gtcatgatga tcattacttt
    29941 atccacgccg accgcctgct ggaaatgcgc aacgggcaac ttagcgagct gacgggcgaa
    30001 gagcgcgatg ccgcttcgcg tgatgccgtt gcccggacgg cataatatta cgacgctgac
    30061 ctggcggact gctgccgtca ggtcaatatg ct
//
LOCUS       DAMZII010000050        29782 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZII010000050 DAMZII010000000
VERSION     DAMZII010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.050"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(53..1882)
                     /gene="glmS"
                     /locus_tag="POA77_002171"
     CDS             complement(53..1882)
                     /gene="glmS"
                     /locus_tag="POA77_002171"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI6365609.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2044..3414)
                     /gene="glmU"
                     /locus_tag="POA77_002172"
     CDS             complement(2044..3414)
                     /gene="glmU"
                     /locus_tag="POA77_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI6365610.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3765..4184)
                     /gene="atpC"
                     /locus_tag="POA77_002173"
     CDS             complement(3765..4184)
                     /gene="atpC"
                     /locus_tag="POA77_002173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI6365611.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4205..5587)
                     /gene="atpD"
                     /locus_tag="POA77_002174"
     CDS             complement(4205..5587)
                     /gene="atpD"
                     /locus_tag="POA77_002174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI6365612.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5614..6477)
                     /gene="atpG"
                     /locus_tag="POA77_002175"
     CDS             complement(5614..6477)
                     /gene="atpG"
                     /locus_tag="POA77_002175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI6365613.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6528..8069)
                     /gene="atpA"
                     /locus_tag="POA77_002176"
     CDS             complement(6528..8069)
                     /gene="atpA"
                     /locus_tag="POA77_002176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI6365614.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8082..8615)
                     /gene="atpH"
                     /locus_tag="POA77_002177"
     CDS             complement(8082..8615)
                     /gene="atpH"
                     /locus_tag="POA77_002177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI6365615.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8630..9100)
                     /gene="atpF"
                     /locus_tag="POA77_002178"
     CDS             complement(8630..9100)
                     /gene="atpF"
                     /locus_tag="POA77_002178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI6365616.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9162..9401)
                     /gene="atpE"
                     /locus_tag="POA77_002179"
     CDS             complement(9162..9401)
                     /gene="atpE"
                     /locus_tag="POA77_002179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI6365617.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9448..10263)
                     /gene="atpB"
                     /locus_tag="POA77_002180"
     CDS             complement(9448..10263)
                     /gene="atpB"
                     /locus_tag="POA77_002180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI6365618.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10272..10652)
                     /gene="atpI"
                     /locus_tag="POA77_002181"
     CDS             complement(10272..10652)
                     /gene="atpI"
                     /locus_tag="POA77_002181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDI6365619.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11269..11892)
                     /gene="rsmG"
                     /locus_tag="POA77_002182"
     CDS             complement(11269..11892)
                     /gene="rsmG"
                     /locus_tag="POA77_002182"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI6365620.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11956..13845)
                     /gene="mnmG"
                     /locus_tag="POA77_002183"
     CDS             complement(11956..13845)
                     /gene="mnmG"
                     /locus_tag="POA77_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI6365621.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14224..14667)
                     /gene="mioC"
                     /locus_tag="POA77_002184"
     CDS             complement(14224..14667)
                     /gene="mioC"
                     /locus_tag="POA77_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDI6365622.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14757..15215)
                     /gene="asnC"
                     /locus_tag="POA77_002185"
     CDS             complement(14757..15215)
                     /gene="asnC"
                     /locus_tag="POA77_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDI6365623.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15367..16359
                     /gene="asnA"
                     /locus_tag="POA77_002186"
     CDS             15367..16359
                     /gene="asnA"
                     /locus_tag="POA77_002186"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDI6365624.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16364..17815)
                     /gene="viaA"
                     /locus_tag="POA77_002187"
     CDS             complement(16364..17815)
                     /gene="viaA"
                     /locus_tag="POA77_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDI6365625.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17809..19305)
                     /gene="ravA"
                     /locus_tag="POA77_002188"
     CDS             complement(17809..19305)
                     /gene="ravA"
                     /locus_tag="POA77_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDI6365626.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19528..21396
                     /gene="kup"
                     /locus_tag="POA77_002189"
     CDS             19528..21396
                     /gene="kup"
                     /locus_tag="POA77_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDI6365627.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21563..21982
                     /gene="rbsD"
                     /locus_tag="POA77_002190"
     CDS             21563..21982
                     /gene="rbsD"
                     /locus_tag="POA77_002190"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI6365628.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21990..23495
                     /gene="rbsA"
                     /locus_tag="POA77_002191"
     CDS             21990..23495
                     /gene="rbsA"
                     /locus_tag="POA77_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDI6365629.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23500..24465
                     /gene="rbsC"
                     /locus_tag="POA77_002192"
     CDS             23500..24465
                     /gene="rbsC"
                     /locus_tag="POA77_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDI6365630.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24490..25380
                     /gene="rbsB"
                     /locus_tag="POA77_002193"
     CDS             24490..25380
                     /gene="rbsB"
                     /locus_tag="POA77_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDI6365631.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25506..26435
                     /gene="rbsK"
                     /locus_tag="POA77_002194"
     CDS             25506..26435
                     /gene="rbsK"
                     /locus_tag="POA77_002194"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6365632.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26439..27431
                     /gene="rbsR"
                     /locus_tag="POA77_002195"
     CDS             26439..27431
                     /gene="rbsR"
                     /locus_tag="POA77_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDI6365633.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27397..28824)
                     /gene="mdtD"
                     /locus_tag="POA77_002196"
     CDS             complement(27397..28824)
                     /gene="mdtD"
                     /locus_tag="POA77_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDI6365634.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28847..29539)
                     /locus_tag="POA77_002197"
     CDS             complement(28847..29539)
                     /locus_tag="POA77_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI6365635.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7164 a         7646 c         7603 g         7369 t
ORIGIN      
        1 agttataaaa aagggccacc ggtcggcagc ccttttttat ctatgatagt gattactcaa
       61 ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga
      121 catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca
      181 catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg
      241 cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt
      301 ccaacagttc gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca
      361 gcgggccgtg tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt
      421 tcagcttcaa tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg
      481 cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagacagca
      541 tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg
      601 catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag
      661 tggtgaatgc tttagtggat gccacgccga tttccgtacc cgcgttggtc atcagcgcca
      721 gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt
      781 aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg
      841 acaaggtgat catcaggctg ttacgacgca cggcagattt gcgatagcgg aattcagagg
      901 cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtaacgg gaaaccatac
      961 cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt
     1021 cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa
     1081 gggtgttttt gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa
     1141 tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg
     1201 cgccagtttt atcgaagatg tttaccgaac ggcgagtaat ttccgcaata tcgccctctt
     1261 caaggaagat aaagcgacgg gtcaccggca gcagcgccag ctggtcagaa gcgataaagt
     1321 tttcgcccat acccagacca atcaccagcg gactaccaga acgtgccgcc agcagggtat
     1381 ccgggtgacg ggagtccatg atcactgtac cgtacgcacc acgcagttgc gggatagcac
     1441 gcagaacagc ctcacgcaga gtaccgccct gtttcagctc ccagttcacc agatgggcaa
     1501 ttacttcggt gtctgtttca gaaacgaagg tatagccacg cgcttttaac tcttcgcgca
     1561 gcggttcatg gttttcgatg atgccgttat gcaccaccac aatgtgttca gaaacatgcg
     1621 gatgcgcatt cacttctgaa ggttcaccgt gggtcgccca gcgagtatga gcaatacccg
     1681 taccgccatg cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc
     1741 gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc
     1801 cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga
     1861 tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct
     1921 gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta
     1981 ctgcttgtgg gagggagatt atgttatctc ctcatcccat gtgaccggat tacccggcca
     2041 gaatcacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct
     2101 gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc
     2161 gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc
     2221 gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc
     2281 gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac cagctttcga
     2341 gcctttaccc agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg caccttccag
     2401 caactcagca ccaggacgca gtcgggcaaa cgggccaata gtacaggccg ctgccagatt
     2461 cgcgtcttcc acaacggtat acggactgat ttcgcaatca tcaccaatca cgctgttttt
     2521 aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat
     2581 aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca aatcaaaacg
     2641 cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta
     2701 aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg
     2761 ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt
     2821 gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc
     2881 tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt
     2941 ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata
     3001 accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc
     3061 acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat
     3121 gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg
     3181 ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc
     3241 gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac
     3301 atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat
     3361 gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt
     3421 cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt
     3481 catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt
     3541 gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt
     3601 aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg
     3661 tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa
     3721 gccagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg
     3781 gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg
     3841 taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg
     3901 gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg
     3961 ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt
     4021 ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag
     4081 atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt
     4141 tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa
     4201 ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg
     4261 cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt
     4321 ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct
     4381 gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca
     4441 gttcatccat acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg
     4501 actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac
     4561 ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc
     4621 tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt
     4681 catccgcagg tacgtatacc gcctgtacgg aggtgataga accagttttg gtggaggtga
     4741 tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag
     4801 gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt
     4861 caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg
     4921 tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata
     4981 ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac
     5041 gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga
     5101 gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc
     5161 ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag
     5221 agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac
     5281 cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg
     5341 ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca
     5401 gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa
     5461 cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta
     5521 cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag
     5581 tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat
     5641 ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc
     5701 tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg
     5761 ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag
     5821 cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt
     5881 cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga
     5941 catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta
     6001 ggcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat
     6061 gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga
     6121 gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag
     6181 cagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac
     6241 caggtagccc acgcgtttaa cgtcgcgttc ttccaggtat gggtgcttat attccagatt
     6301 accgtgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc
     6361 catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat
     6421 cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag
     6481 cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg
     6541 gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca
     6601 ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga
     6661 gcggcttcga agctgccaat tttcgacagt tcaacatccg ccaggtaacc acgttctgct
     6721 gcgaacagaa ccagagactg ctgcgcaacg gacatcggcg catactgttt ctgtttcagc
     6781 agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat
     6841 gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg
     6901 gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc
     6961 gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca
     7021 tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg
     7081 ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct
     7141 tcaacgtatt cggcgttaac acgtgcagca cgctccagca gacgagagtg gaggtagaaa
     7201 acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg
     7261 taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca
     7321 cggaagtatt cgcccattgc gcaaccggca tacggtgcca ggtattgcag tgcagcggat
     7381 tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta
     7441 cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata
     7501 ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc tgtctgacgg
     7561 tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta
     7621 taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct
     7681 tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga
     7741 gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta
     7801 cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca
     7861 gagtcgcgct cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca
     7921 ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata
     7981 gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt
     8041 tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct
     8101 gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg
     8161 ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga
     8221 cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa
     8281 atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaga
     8341 acatccggga gcgcgttaag acgaccattt tcagccatta cccgaatcag gttctgaccg
     8401 ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt ttctggcgca
     8461 agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc
     8521 agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg
     8581 gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag
     8641 cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct
     8701 tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt
     8761 tacgctcggc ttcaatttct gcctgcgcct gggccacgat tttagtacgt tcctgttctg
     8821 cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta
     8881 cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa
     8941 ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt
     9001 cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa
     9061 acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag
     9121 ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc
     9181 acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata
     9241 aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat
     9301 ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca
     9361 gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt
     9421 cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac
     9481 gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc
     9541 ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa
     9601 aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccaagtgaaa ctggtttgga
     9661 cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg
     9721 ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag
     9781 aatcaggata aatacgccca atgccataga cagcgttacg ttcacgtccg cagacggaac
     9841 cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg
     9901 cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc
     9961 aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac
    10021 cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct
    10081 acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt
    10141 gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc
    10201 cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc
    10261 catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc
    10321 accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc
    10381 accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc
    10441 acccggcctt tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg
    10501 ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg
    10561 aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga
    10621 gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag
    10681 gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt
    10741 caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg
    10801 tttaaatatt tttccagtgc atacaattgc gacttttctg ctaaccctgt tcgatcatga
    10861 aaaactgtaa ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc
    10921 atgttcgcgc acagcatatt tatttacttg gcaaatgatg cctttgcaag tttatgatat
    10981 ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa aaccacccat
    11041 tgacattttt aataatgttt taacagccaa tgatggttct tagcgccgat ttttagcaga
    11101 ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg
    11161 atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg
    11221 ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt
    11281 tgctttaatc accaccagat gacgttcgcc atccagggct ggaacctgaa gtttaaccac
    11341 tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt
    11401 cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt
    11461 cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc
    11521 taccctgctc tgtactggtt caatattctc cagtttaagc tcatgttgca cctgacgaag
    11581 gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat
    11641 agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg
    11701 cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc
    11761 gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat
    11821 aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca gtaaggagag
    11881 tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca
    11941 ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag
    12001 cagaatggag atggccgcag gcgtgacgcc agaaatacgc gaagcctggc cgatagaggc
    12061 tggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta
    12121 atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg
    12181 ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc
    12241 gtctgtcaat gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg
    12301 acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt
    12361 cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc
    12421 acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccactaggcc
    12481 cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgta gcatcagacg
    12541 atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc
    12601 atcaactagt acgccgagat acgcctgaga acgtgccgga gcccaacctt ctttgtcagc
    12661 agacagacgg gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc
    12721 ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt
    12781 cggtttcagg tcgcgaggat cgaagaagtc atactcaatg gcataacccg gacgcacgat
    12841 cttcgcgttt tccatcccct gcatagagcg gacgatttgc atctgcacat cgaacggcag
    12901 gctggtggag ataccgttcg gataaatttc attagaggtc agtccttccg gttcaaggaa
    12961 gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg acgggcagta
    13021 gcgtgggccg acaccttcga tcacccctgc atacattggg ctacgatcga ggttactgcg
    13081 gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg
    13141 ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc
    13201 cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg
    13261 accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc
    13321 agcacggcca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga ggaacgtccc
    13381 aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac
    13441 cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg
    13501 ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg
    13561 ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga tacccgcttg
    13621 atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt
    13681 cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt
    13741 caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg
    13801 acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca
    13861 taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct
    13921 tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc
    13981 tattgtgatc tcttattagg atcgcactgc cctgtggata acaaggatcc ggcttttaag
    14041 atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc
    14101 tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg ttctttggat
    14161 aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta
    14221 tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat
    14281 gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt
    14341 gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat
    14401 accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata
    14461 gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taatcagcca
    14521 gattcccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc
    14581 cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct
    14641 gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt
    14701 acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag
    14761 ggcttgatgg tacgcatgat cgggttttgc aggacgatca atgtctcggt ggactgaatt
    14821 tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac
    14881 atcactttta taaagatgct gtagtggccg gttgtgtaat aggcttcagt gacttcatca
    14941 aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa tataatgccg
    15001 ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta
    15061 atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca
    15121 aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg
    15181 ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc aaattgatga
    15241 ttcattctat tttagccttc ttttttaatg aatcaaaagt gtgttaggct ttttattgaa
    15301 tgattattgc atgtgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta
    15361 taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac
    15421 ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc
    15481 cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa
    15541 gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt
    15601 cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc
    15661 cttcgccccg atgaagagcg tctttctccg ttgcactcgg tctatgttga ccagtgggac
    15721 tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag
    15781 gcgatctggg cgggaattaa agcaaccgaa gctgcagtta gcgaagagtt tggtttggca
    15841 ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg
    15901 gatctcgatg ccaaagggcg tgagcgggcg attgcgaaag atcttggcgc ggtattcctc
    15961 gtcgggattg gcggcaagct gagtgatggt catcgtcacg acgtgcgcgc accggattat
    16021 gatgactgga gcaccccgtc agagctgggc catgcgggtc tgaacggcga tattctggta
    16081 tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc
    16141 gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg
    16201 catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct
    16261 cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg tggtgtatgg
    16321 ccagctgctg ttcgcgagaa cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc
    16381 tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga
    16441 tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct
    16501 gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa
    16561 tcaccaccgc gtcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa
    16621 aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt
    16681 cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat
    16741 agcagcgccg gttttcagcg agagcaatgc gcatcaaggc caggcagaac gctttcgcac
    16801 actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata aacggcccgc
    16861 gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt tcacgccacg
    16921 actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat
    16981 actccagttc cgttatccct agtgtcgcca gttctggcgg caggagacgt aaaatatcat
    17041 cgctttgttg cagaccatca acctgctcag gaacagttgc cggttcgcgc accatggtgc
    17101 ggaaggcttc catctgcgca tcgttacgcg gtattgattt ggcttcccgg gaacgcccca
    17161 gctgttctgc caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa
    17221 tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga cgaccagctg
    17281 cggtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa
    17341 cttcacttaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac gtcgttgctt
    17401 gcacgatcag acttaaacgc caacgctgga ggaaaagcgt atgtaacgct gaagtgatcg
    17461 tgctgttagc atcaaccaac tgtcgggctt gttctgccca tggggaattc agacgatgca
    17521 gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt
    17581 gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgac
    17641 tgcgcagcgc ctcacgccag cggggaacat catcagtgat tgccgccttc agtcgtggga
    17701 atttttcaaa gaagactgcc agctgcggtg aggccagcag cgcgatgatc atctcttcga
    17761 tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg
    17821 tgcctggcga atctgtgcgc cgacatcctg taggctggct tcaatgcgac ctaaccagtc
    17881 gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct tgcgccagtc
    17941 actttcaagc tcttccagtt gttgttttat ttcacctggc agaccttcag ccgatgaacc
    18001 gggaagtgcc agcgtactgc cttgtaaact tacatcgcgt acaacaagat gttgggcgct
    18061 atccacttcc agattcaatt tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc
    18121 accaccttta ctcagccact gttccagcgc gttacgctca aaggagatat gaaccacttc
    18181 catatcatgc aattttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt
    18241 aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa
    18301 ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgccaag
    18361 gcgagtcaac atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg
    18421 ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc
    18481 taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata atcggatcgc
    18541 ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg
    18601 cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg
    18661 ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc
    18721 atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca
    18781 cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc
    18841 gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac cgttgcggaa
    18901 ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat
    18961 ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg
    19021 cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa
    19081 gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc
    19141 gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc
    19201 cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact
    19261 gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc
    19321 tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata
    19381 gtctcaaagg gggggtattt ttctttgagc caggttaatg tggccgcatt taggagtacg
    19441 attttgccgt taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca
    19501 tttcatattt caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg
    19561 attaccctcg cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca
    19621 cttcgtgaat gtttgtccgg ccagtttggt tttggcgttg aacgcgatgc cgtgtttggc
    19681 tttttatcgc tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc
    19741 gtgatgcggg cagataacgc cggtgagggg gggatcctga cgttgatgtc gcttgccggg
    19801 cgtaatacgt cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagc
    19861 tttttctatg gtgaagtcgt cataacaccc gctatttcgg tgatgtcagc cattgaaggt
    19921 ctggaaatcg tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc
    19981 actttattat ttatgattca aaaacatggc acggctatgg tcggtaagct gtttgcgccg
    20041 atcatgctga cctggttttt gattctggca ggactggggt tacgtagcat tattgctaac
    20101 ccggaagtgc tgcatgcgct gaatccaatg tgggcggtgc atttcttcct tgaatacaaa
    20161 acggtttctt ttattgcatt aggggcagtg gtgttgtcga ttacgggggt cgaggcgctg
    20221 tatgctgata tggggcactt tggtaagttc cctattcgcc tggcgtggtt caccgtcgta
    20281 ttaccttcct tgacccttaa ttacttcggt cagggagcgc tgttgttaaa gaacccggaa
    20341 gcgattaaga acccgttctt cctgttggca ccggactggg cgctgatccc gctgctgatc
    20401 atcgccgcac tggcgacggt aatcgcctcg caggctgtca tctctggcgt cttctcattg
    20461 acgcgtcagg cggtacgtct gggatatttg tcgccgatgc gcattattca cacctccgaa
    20521 atggagtcag ggcaaatcta tattccgttt gtgaactgga tgctctatgt cgcggtcgtg
    20581 attgtgattg tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg
    20641 accggaacca tggtgctgac gtctattctc tcgactactg tggcacgtca gaactggcac
    20701 tggaataagt attttgttgc gctgatcctg attgctttcc tttgtgtcga tattccattg
    20761 ttcaccgcta acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtact
    20821 gtgatgttta tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg
    20881 catgaacatg gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcctgtt
    20941 cgcgtgcccg ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg
    21001 atgcataacc ttaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgc
    21061 accgaagacg cgccatatgt ccataacgtc cgtcgggtac agatagaaca actgtcgccc
    21121 actttctggc gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt
    21181 ttccaccgct gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg
    21241 tcgcatgagt cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg
    21301 tacttgctgc tgcaacgtaa tgcgctgcgt gcaccagatc aatttgaaat tccgccaaac
    21361 agggttatcg aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttctccata
    21421 agggggcgtt tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat
    21481 ttccgtaacg tcacgatggt tttcccaact cagtcaggat taaactgcgg gtcagcgaaa
    21541 cgtttcgctg atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc
    21601 gatgatctcc cgtctgggac ataccgatac gctggtggtg tgtgatgctg gtttacccat
    21661 ccccaaaagt acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca
    21721 ggtgctgggc gtcgtcacaa atgaaatgca ggtcgaggct gccattatcg cggaagagat
    21781 caaacaacat aatccgcaac tccacgaaac gttgctcact caccttgagc agctgcaaaa
    21841 acaccaggga aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac
    21901 cgcagaaagt caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct
    21961 ctgtgctggc gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg
    22021 ataaagcctt cccgggcgta aaagccctct cgggcgcagc gttaaatgtc tatccgggcc
    22081 gcgtgatggc gctggtgggc gaaaacggcg cgggtaaatc caccatgatg aaagtgctta
    22141 ctggtatcta tgctcgcgat gccggcacgc ttttatggct ggggaaagaa acgacattta
    22201 ccgggccgaa atcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga
    22261 tcccgcagtt gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg
    22321 gcaaaattga ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc
    22381 tgcgctttaa aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg
    22441 aaatcgccaa agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg
    22501 cgctgaccga taccgaaacc gaatccctgt tccgcgtcat acgcgagctg aaatcgcaag
    22561 gccgcggtat tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg
    22621 ttaccgtttt tcgtgacggg caatttattg ctgagcgcga agtggcatca ctgaccgaag
    22681 attcgctgat tgagatgatg gtgggccgca agctagaaga tcaatatcca cacctggaca
    22741 aagcgccggg agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg
    22801 tctcttttac tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc
    22861 gtaccgaact gatgaaagtg ctctacggcg cactaccgcg caccagcggt tacgtcaccc
    22921 tggatgggca tgaagtcgtt acccgttcac cgcaggatgg cctggcaagc ggcattgtgt
    22981 atatctccga agaccgtaaa cgtgacggtt tagtgttggg catgtcagta aaagagaaca
    23041 tgtcgctgac agcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg
    23101 aacagcaggc tgtgagtgat ttcattcgtc tgtttaatgt gaaaacaccg tcgatggaac
    23161 aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga
    23221 tgacacgccc caaagtgttg atccttgatg aacctacccg tggcgtagat gtcggcgcga
    23281 aaaaagagat ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg
    23341 tgtcatcgga gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag
    23401 ggcatctcag cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg
    23461 ctgtgggcaa gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct
    23521 ggtcgccgtt atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg
    23581 gtgctgatcg cgattgtctc gacgttaagc ccgaactttt tcaccatcaa taacttattc
    23641 aatattctcc agcaaacctc ggtgaacgcc attatggcgg tcgggatgac gctggtgatc
    23701 ctgacgtcgg gcatcgactt gtctgtaggt tctctgttgg cgctaaccgg cgcagttgct
    23761 gcatctatcg tcggcattga agtcaatgcg ctggtggctg tcgctgccgc tctcgcgtta
    23821 ggtgccgcaa ttggtgcggt aaccggggtg attgtagcga aaggtcgcgt tcaggcgttt
    23881 atcgctacgc tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt
    23941 agcccagtga ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt
    24001 cgtccgctgg gcgtaccgac gccagtctgg atcatgggga ttgtcttcct cgcggcctgg
    24061 tacatgctgc atcacacgcg tctggggcgt tacatctacg cgctgggcgg caacgaagcg
    24121 gcaacgcgtc tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt
    24181 ggtctgctgg catcgctggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc
    24241 acggcgggga ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt
    24301 ctggcgggcg gaaaaggtcg cattgttggg acgttgatcg gcgcattaat tcttggcttc
    24361 cttaataatg gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg
    24421 gtggtgattt tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac
    24481 atcttgaata tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc
    24541 accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc cacgcttaac
    24601 aacccgttct ttgtatcgct gaaagatggc gcgcagaaag aggcggataa gcttggctat
    24661 aacctggtgg tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtgcaggac
    24721 ttaaccgttc gcggcaccaa aattctgctg attaacccga ccgactccga cgcagtaggt
    24781 aatgctgtga agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca
    24841 acgaaaggtg aagtggtgag ccacattgct tctgataacg tactgggtgg caaaatcgcc
    24901 ggtgattaca tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct gcaaggcatt
    24961 gctggtacat ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt tgctgctcac
    25021 aagtttaatg ttcttgccag ccagccagca gattttgatc gcactaaagg tttgaacgta
    25081 atgcagaacc tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa
    25141 atggcgctgg gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc
    25201 ggatttgacg gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact
    25261 atcgctcagc tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg
    25321 aaaggcgaga aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag
    25381 ttttaatcag gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt
    25441 aatataaaga aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc
    25501 cgaatatgca aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc
    25561 ttaatcttca atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg
    25621 catttggcgg caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg
    25681 cgtttattgc ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca
    25741 ctgataacat tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc
    25801 tgatttttgt taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg
    25861 ccctttcccc ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat
    25921 taatgcagct ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa
    25981 ataagactat cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg
    26041 cgctggtgga cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg
    26101 ttgaaaatga tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta
    26161 ctgtactgat tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc
    26221 gcgttcctgg attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg
    26281 gcgcgttaat cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc
    26341 atgctgccgc tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg
    26401 aagagatcga cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg
    26461 cccgcctggc gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg
    26521 tcagtgaagc gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat
    26581 cagccctggc gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg
    26641 ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac
    26701 gcggttatag tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc
    26761 tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc
    26821 aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg
    26881 ctccgttcga tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag
    26941 caacgcaata tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg
    27001 ataaaactcc ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc
    27061 tcagcattcc tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg
    27121 ccatgcgcca actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg
    27181 ctatggctgt tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata
    27241 tcgcggtgat tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca
    27301 ctatccacca accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga
    27361 taacccagcc gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg
    27421 gttcggctta gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag
    27481 catgaacatt gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa
    27541 ttgttcgaca gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac
    27601 agcaacgcct aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct
    27661 ggcgttgtca tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga
    27721 catagccatc cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga
    27781 ttgcaaagag aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg
    27841 gcgatagccc agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa
    27901 cgctgtcggt gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa
    27961 catcaatggc ataaggaacg gtacacaacc ggtccccaga cgggttgcaa tattgcctac
    28021 gataccgatc gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt
    28081 gtgtcgcgca tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa
    28141 ggcaatccag ctggcgacaa tcctttcccc gaatagctct attccgcttg agaagagaac
    28201 aaggctgagg ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa
    28261 attgggcata tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa
    28321 aaatatccag tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat
    28381 tggccccacc agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg
    28441 ataagcgcgc agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg
    28501 aataacccgg aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc
    28561 caatgtgaac agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag
    28621 ccatccgctt accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg
    28681 catcgcgaga ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat
    28741 agtggcatca agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat
    28801 actgcgcttc tttttatcgc tcattcaatg tcctgttatc gggttgtcac ttatcaggtg
    28861 agcgtagcag cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat
    28921 cgacaatcgc ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt
    28981 aagtgtgata aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca
    29041 tttcatagat atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt
    29101 tctcttttaa tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg
    29161 tgccaaccgt tgcagccagt aggcaagctt gcggctccag acagatacgc ataaccagaa
    29221 agtgatcgat gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat
    29281 caagaaaatt ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa
    29341 ccatcccttt tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa
    29401 attgctcgcc cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta
    29461 agatgcgttg cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact
    29521 gttgtgcgct taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc
    29581 accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac
    29641 ggggcttttg ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc
    29701 cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
    29761 gtcagcgggg ttctcctgag aa
//
LOCUS       DAMZII010000051        29627 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZII010000051 DAMZII010000000
VERSION     DAMZII010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.051"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..198)
                     /locus_tag="POA77_002198"
     CDS             complement(<1..198)
                     /locus_tag="POA77_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDI6365636.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGI"
     gene            complement(201..920)
                     /gene="sanA"
                     /locus_tag="POA77_002199"
     CDS             complement(201..920)
                     /gene="sanA"
                     /locus_tag="POA77_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDI6365637.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1070..1954)
                     /gene="cdd"
                     /locus_tag="POA77_002200"
     CDS             complement(1070..1954)
                     /gene="cdd"
                     /locus_tag="POA77_002200"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI6365638.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2084..2779)
                     /locus_tag="POA77_002201"
     CDS             complement(2084..2779)
                     /locus_tag="POA77_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDI6365639.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2776..3174)
                     /locus_tag="POA77_002202"
     CDS             complement(2776..3174)
                     /locus_tag="POA77_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDI6365640.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3413..4360
                     /gene="dusC"
                     /locus_tag="POA77_002203"
     CDS             3413..4360
                     /gene="dusC"
                     /locus_tag="POA77_002203"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDI6365641.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(4733..4816)
                     /gene="yohP"
                     /locus_tag="POA77_002204"
     CDS             complement(4733..4816)
                     /gene="yohP"
                     /locus_tag="POA77_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDI6365642.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5040..6476
                     /gene="mdtQ"
                     /locus_tag="POA77_002205"
     CDS             5040..6476
                     /gene="mdtQ"
                     /locus_tag="POA77_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDI6365643.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            6529..7290
                     /locus_tag="POA77_002206"
     CDS             6529..7290
                     /locus_tag="POA77_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6365644.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(7420..7998)
                     /locus_tag="POA77_002207"
     CDS             complement(7420..7998)
                     /locus_tag="POA77_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6365645.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            8168..8755
                     /locus_tag="POA77_002208"
     CDS             8168..8755
                     /locus_tag="POA77_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDI6365646.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            8929..9861
                     /gene="pbpG"
                     /locus_tag="POA77_002209"
     CDS             8929..9861
                     /gene="pbpG"
                     /locus_tag="POA77_002209"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDI6365647.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9899..11614)
                     /gene="dld"
                     /locus_tag="POA77_002210"
     CDS             complement(9899..11614)
                     /gene="dld"
                     /locus_tag="POA77_002210"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6365648.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            11810..14107
                     /gene="bglX"
                     /locus_tag="POA77_002211"
     CDS             11810..14107
                     /gene="bglX"
                     /locus_tag="POA77_002211"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDI6365649.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14318..15235
                     /gene="osmF"
                     /locus_tag="POA77_002212"
     CDS             14318..15235
                     /gene="osmF"
                     /locus_tag="POA77_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDI6365650.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            15242..16399
                     /gene="yehY"
                     /locus_tag="POA77_002213"
     CDS             15242..16399
                     /gene="yehY"
                     /locus_tag="POA77_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDI6365651.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            16392..17318
                     /gene="yehX"
                     /locus_tag="POA77_002214"
     CDS             16392..17318
                     /gene="yehX"
                     /locus_tag="POA77_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDI6365652.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            17323..18054
                     /gene="yehW"
                     /locus_tag="POA77_002215"
     CDS             17323..18054
                     /gene="yehW"
                     /locus_tag="POA77_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDI6365653.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(18035..18142)
                     /locus_tag="POA77_002216"
     CDS             complement(18035..18142)
                     /locus_tag="POA77_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDI6365654.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(18202..18933)
                     /gene="mlrA"
                     /locus_tag="POA77_002217"
     CDS             complement(18202..18933)
                     /gene="mlrA"
                     /locus_tag="POA77_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDI6365655.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            19155..20840
                     /gene="btsS"
                     /locus_tag="POA77_002218"
     CDS             19155..20840
                     /gene="btsS"
                     /locus_tag="POA77_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDI6365656.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            20837..21556
                     /gene="yehT"
                     /locus_tag="POA77_002219"
     CDS             20837..21556
                     /gene="yehT"
                     /locus_tag="POA77_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDI6365657.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            21603..22073
                     /locus_tag="POA77_002220"
     CDS             21603..22073
                     /locus_tag="POA77_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDI6365658.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(22114..22575)
                     /locus_tag="POA77_002221"
     CDS             complement(22114..22575)
                     /locus_tag="POA77_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDI6365659.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(22700..24700)
                     /locus_tag="POA77_002222"
     CDS             complement(22700..24700)
                     /locus_tag="POA77_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6365660.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAVECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(24697..25833)
                     /locus_tag="POA77_002223"
     CDS             complement(24697..25833)
                     /locus_tag="POA77_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6365661.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQVDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(25826..28105)
                     /locus_tag="POA77_002224"
     CDS             complement(25826..28105)
                     /locus_tag="POA77_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365662.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(28116..29204)
                     /locus_tag="POA77_002225"
     CDS             complement(28116..29204)
                     /locus_tag="POA77_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI6365663.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         7046 a         7577 c         8035 g         6969 t
ORIGIN      
        1 aattccgaag ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg
       61 ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca
      121 gcctttccat gcttcgcgga aacaaaatat cggaataagg aaaaaaacgg caacgacaaa
      181 aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg
      241 ggtgtgacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg
      301 accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg
      361 aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca
      421 taacactgag cctgaatccc catatgcagc gcaataaata atgcccgctc acagtggaaa
      481 cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa
      541 tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg
      601 attaaatctt tgcgcatggt catcggctca ttataacttt gcaatgcgtt atcgccgctc
      661 agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag
      721 cgataatact gattaattac gccagtacga taatattttg ctgttccgag caccacaccg
      781 acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc
      841 atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg
      901 aggaacacgc gctttaacat gcaggagact cgatctaagg aaaatttcca tcaggctact
      961 ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga
     1021 gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa
     1081 gcactcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact
     1141 gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat
     1201 aacccttgag attcaacaga attaacgctc cctgcaacgg tggcagagtc gggttgaatg
     1261 cggcgttttc agcgtagctg ccactgaaaa tacgaccgtc tttacattcc agcgcgacac
     1321 cgcttggcga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag
     1381 aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa
     1441 tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc
     1501 ggcccggcag atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc
     1561 agtgaccaca aggcgtgtag ttaacggtga tggctgcaag cgctttttca ccactcaacc
     1621 aggcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc gcaccaataa
     1681 attccatatt ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat
     1741 taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca
     1801 gcgcgtcttc gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca
     1861 aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa
     1921 gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg
     1981 gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca
     2041 acgcgtgaaa cttacatcaa aaatgcgcga cgcatcgcaa attttagccc attactgcca
     2101 gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg
     2161 ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg
     2221 cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat
     2281 taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc
     2341 tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg
     2401 atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag
     2461 tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc
     2521 gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt
     2581 gcagcagatc gtttagcact tcgctacctt taaagtagct gtcgtaagag atgccggtca
     2641 gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact
     2701 tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc
     2761 accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg
     2821 taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga
     2881 aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc
     2941 aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca
     3001 tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct
     3061 gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata
     3121 aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa
     3181 caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat
     3241 taagaaatca gcgcttattt tttcgctccc cggcattacg caatgcgcaa cgaactcagg
     3301 catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgt agacctttaa
     3361 tccgctgacc ataggcgcta acatagcgcc tcattatttt gcgggtgatg atatgcgtgt
     3421 gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt
     3481 taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt
     3541 aaaagttttt catcgcattt gccctgagct acaaaacgcc agccggacac catccggtac
     3601 gctggtgcgc gtgcagttgt tgggtcagtt cccacaatgg ctggcagaga acgccgcccg
     3661 tgcggtggaa ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt
     3721 caacggtagc ggtggtgggg cgacgttact caaagatcct gaactcatct atcagggtgc
     3781 aaaagcgatg cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg
     3841 ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggtgctac
     3901 ggagctggtg gtgcatggac ggacaaaaga gcagggttac cgcgcggagc atattgactg
     3961 gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca acggcgaaat
     4021 ctgggactgg cagagcgcgc aacaatgcat tgcgatcagc ggctgcgacg cagtgatgat
     4081 tggtcgcggg gcgctcaata ttcccaacct gagccgggta gtaaaatata acgaaccgcg
     4141 aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg
     4201 cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga
     4261 atacgatgaa gcaacgggat tatttcagca tgttcgggtg ttgaataatt cccctgatat
     4321 tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaaccatag tgatgttttt
     4381 tgacaatgtg ttagctcttt atcaataggc cgcacggttg tttggtggcg tcaaaattgc
     4441 cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgttttac
     4501 agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagccgagg gcaccgcgca
     4561 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgatt ggccgccccc
     4621 tgcgcgctcc ttgcgccagt gattatatga agcaatgcac atgacaggag cgcaacagga
     4681 tgagagactg aaatattctc gttattttcg gacctgatga cattaattaa ttttaaaata
     4741 tcatcttaaa tacgccagtc accaccagta gcccaatcag gaaaataatc aatacagccc
     4801 agagtataat tttcattggc gttcctttat ttgtgagtgc tgaataagta taggaaaaaa
     4861 cgaagccctt tgccgttatc cataaacgat taagataaat cggaagatat cattcactta
     4921 gtgtatattt ttctggatat gtcgttttat tgctactgat gtattgccac gcgaaactaa
     4981 aaaatatatc gcaacacacg gtaatctccg cgcacctttt cttacctgat tattccccca
     5041 tgaatcgtga ttccttttat cccgccatcg cctgttttcc gctgttactg atgctggccg
     5101 ggtgtgcacc tatgcatgaa acccgccagg cgttaagcca gcaaacgcct gctgcacaag
     5161 ttgacaccgc attacccacg gcgctgaaaa atggttggcc agacagccaa tggtggctgg
     5221 agtatcacga taatcaactc acttccttaa ttaacaatgc actacagaac gcaccggata
     5281 tgcaggtggc tgaacagcgc attcagctag ctgaagccca ggcgaaggca attgctactc
     5341 aggatggtcc gcagatcgac ttttctgcgg atatggagcg gcaaaaaatg tcggcagaag
     5401 gcttaatggg gccgtttgct ctgaacgatc cggccgcagg tacgaccggt ccgtggtaca
     5461 ccaacggaac ttttggctta acggcgggct ggcatctcga tatctgggga aagaatcggg
     5521 cggaggttac tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc gagcaaaccc
     5581 gccaattgct ggctggcagc gtagcccgcc tgtactggga gtggcaaacc caggcggcgt
     5641 taaacacggt cttgcagcaa atagaaaaag agcagaacac cattatcgcg accgatcgcc
     5701 agctatatca gaacgggatt acttcttcag ttgaaggtgt ggaaaccgat attaatgcca
     5761 gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat gaaaattatt gaggcacggt
     5821 taagcgcact tacaaataac cagacaaagt cattgaagct taaaccggtc gcgttgccga
     5881 aagtggcaag ccagcttcct gatgaactgg ggtactcctt actggcccgg cgggcagatt
     5941 tgcaggcggc gcactggtac gttgagtcat cgctaagcac cattgatgcg gcaaaagcgg
     6001 cattttatcc tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg cacttaagcg
     6061 atctgttccg tcattccgcg cagcaaatgg gcgttacggc aggcctgacg ctacccattt
     6121 tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc aacttgtcta
     6181 tcgccagcta caacaaagcg gtggttgaag cggtgaatga cgtggcgcgg gcagccagtc
     6241 aggttcagac acttgcggag aaaaaccagc atcaggcgca aattgagcgc gatgccttgc
     6301 gtgtggtagg tcttgcgcag gcgcgcttta acgcgggcat cattgctggt tcccgcgtca
     6361 gcgaagccag aatccccgcg ctgcgtgagc gggccaatgg cctgttattg caagggcagt
     6421 ggctggatgc ttccattcaa ctcactggtg cgttgggcgg gggttacaaa cgctgatgat
     6481 atgcgccttc tatacttaac gtttattcag cgttaagtgg agaactcgat ggcacaggtt
     6541 gcgattatta ccgcctccga ttcggggatc ggcaaagagt gcgcgttatt actggcgcag
     6601 caggggtttg atattggcat tacctggcac tcagatgaag aaggggcaaa agataccgcg
     6661 cgtgaggtag ttagccacgg cgtacgtgcg gagatcgtgc agctggatct cggtaaactg
     6721 ccagaaggtg cgcaggcgct ggagaaactc attcaacggc tggggcgtat tgatgtgctg
     6781 gtgaataatg cgggcgcaat gaccaaagcg ccgtttcttg atatggcttt cgatgagtgg
     6841 cgcaagattt ttaccgttga tgtcgatggt gcatttttat gctcacaaat tgcggcccgc
     6901 cagatggtga aacaagggca gggcggtcgc atcatcaaca ttacgtcggt acatgaacat
     6961 acgccgctgc cggatgccag cgcctacaca gccgctaaac atgcgctcgg cgggttaacc
     7021 aaagcaatgg cgctggagtt ggtcaggcat aagattttgg tgaacgcagt cgcacctggg
     7081 gcgatcgcta cgccaatgaa cggcatggat gatagctacg tgaagcccga cgcggagcct
     7141 tcgattcctc tgcggcgttt tggcgcaacg catgagattg ccagcctggt agcgtggctg
     7201 tgctcagagg gcgcaaatta caccaccggg cagtcgttga tagtggatgg cggctttatg
     7261 ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg tgcgtggtta tttcaatggg
     7321 taaaattttg gtaggcatga taagacgcac aagcgtcgca tcaggcatgg tgcaccggat
     7381 gtcggatgcg gcgcgaacgc cttacccagg gcaatggttt tacgcctgat gatccggctt
     7441 tttcttcccg cgccgtttca gccaccagcg cacgcccacc accagaacca cgaccagaat
     7501 caaccagacc cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg
     7561 accaccagcg taaccaatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt
     7621 cagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac
     7681 ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc gttggataag
     7741 tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc
     7801 aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccgccaa gcgccacaga
     7861 aagtaccacc agcgggaact ttaatagccc ctgatgcgcc gcaacgcctc ccagcaaagt
     7921 cacggtttca ccttccgcca ggctaccgat caccagcgcg gcataaccat attgtgagat
     7981 aagtgtattg agatccatag gccagtctgt ctccctttgg ttgccagtaa gcatacaccc
     8041 tgtgaatcat tcacgctgaa aaaggacgtt catcagacag tgaaaattac atgggcgaac
     8101 aaaaaataat cacaagctga cttatacttg aactgtcagg ttcatgaact cacaacaagg
     8161 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat
     8221 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
     8281 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
     8341 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
     8401 tatgctggct ggggtcgcgg tgatggggcg ggtcatctgg tggatggcgc gcaattaccc
     8461 gcagcgtccg tcacttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
     8521 tttaagcggt ctggtggcgc tttatccgct ggtctggttg tgtgcgctgg tcggcacggt
     8581 ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc tgaatatcaa
     8641 caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc tggtactgga
     8701 agtgctgctc gccctaaccg ttatactatg gggctacggg tatcgcctgt tctagaaagt
     8761 tctttgacat tgcattgctg gcgtaaatgt cagcaatgca gcatttcctc acgattctcc
     8821 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact
     8881 ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt
     8941 cgagtttctt tatttagcct ggccctgatg ctggctgtgc ctttggcacc gcaggccgtt
     9001 gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg
     9061 attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg
     9121 attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg
     9181 gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc
     9241 gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca
     9301 gaaaaccgcg cggcggcaag ccttgcacac cattatcccg gtggttacaa agcctttatt
     9361 aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct
     9421 accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc
     9481 agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat ggcaaccttc
     9541 tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta tcgcgataac
     9601 tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg tctggtgatg
     9661 cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat
     9721 acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa agtgatgcct
     9781 gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg
     9841 cagacggcac agaacgatta agtgaattcg gatggcgata ctctgccatc cgtaattttt
     9901 actccacttc ctgccagttt ttccgtttac tggttttacc gatccccgga ttcatgctgt
     9961 tggtcggatc gttctcgcga tagaacttct gcaacgtctc cggtgcttta tacaaatgac
    10021 cgacgttatg ctcggcaggg tattgcgcgc cgcgctgctg tagcagttcc agcatctgtt
    10081 cttttaacgc atgcacatcc acgccttttt tcactatgta atcctgatgg aagacatagc
    10141 acataaaatg gccgtaatag agcttgtgca ccagctggct gtcgatctcc ggcggtaaat
    10201 gctcatacca ctcggtgtcg ttacgccgca gagcgatatc caacgccaga atgtcttcga
    10261 cttcatcgga atgcaccgcc tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta
    10321 aaaacgcttt gctgccttcc tccggcgtac agacaaagaa atcgccttcg gcctgtttga
    10381 aataatccac caaccacgat ttggcttcgc ccacgccatc gcccgccatt tttaacagca
    10441 gatgatgctc gtatttatcg cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca
    10501 ggtgaccgaa tttttgcatc gcacggtcag taaaatgcgg acggaagaat ttcactttct
    10561 ccagcatcgc atcggtgcgt cccttgagat taaagaagaa cggcatcttg tcggtgccta
    10621 acttatcaat catcaggaag gtgtctttgc cgtatttttc cgcaatatcg tagatatccc
    10681 ggtgcatata ttccccggca accggcagat tttcgaagtt agccagaata tgacggcgga
    10741 tttcggtcag cacttccggc tggttggtgc cgatataaaa cacctgctga tttttttccg
    10801 cttcgaaggt atctagacgt accgcaaaga cggccagctt cccggcgcaa ccagaagatt
    10861 caaataaccg atccggatcg gcgttataac gtgcgggcgt gtcggcttca atatcacgaa
    10921 cgcggtggac atagtcataa tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt
    10981 tgatgcgatc atcatccagc ttgctaagga tctgctccgg cgtttcgccc agatcaatcc
    11041 ccagatggtt caccagcgtc agtttgccgt cttcatttat acgcgcgaat aacgacattt
    11101 cggtatacgc cgggccacgt tgcaccagcg agccgccgga gttgttacaa ataccgccga
    11161 taaccgatgc gcctatacac gacgatccaa tcaccgagtg cggttcgcgt cccagcggtt
    11221 tgagggcttt ttccagcgaa tagagcgtgg tgcccggata ggccagcacc tgttcgccct
    11281 tgccaagaac gtgcagcttg tcgagacgca gggtgctgat gatcacgata tcgcgatcat
    11341 agtcgttacc gtttggcgtc gatccttcgg tcaggcctgt attggcagcc tgcatcagaa
    11401 taattttgtc ggcggtgaca caggctttca gcacccgcca caattccagt agtgagccag
    11461 ggaaaacgac agccagcgcg tcgccctgac cagaacggaa gcccttgcga tagcgggccg
    11521 tttttgcggg atcggtgagc aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa
    11581 aggctttatt atcagttgtt gtcatggaag acattttcca ctccttgtgg tggcgaaaaa
    11641 atatcacaaa ctagaatagc gccttgtatg acaattcagc gagtgaatat tttgaaaatt
    11701 cagacaataa actgagcatt ctttttctct ctccatcatg cttatggcaa actacggttt
    11761 ttgcaccatt ttcgctgcct cgtcgggcaa cagaggaaga aaaatccata tgaaatggct
    11821 atgttcagta ggaatcgcgg tgagtctggc cctgcagcca gcactggcgg atgatttatt
    11881 cggcaaccat ccattaacgc ccgaagcgcg ggatgcgttc gtcaccgaac tgcttaagaa
    11941 aatgacagtt gatgagaaaa ttggtcagct gcgcttaatc agcgtcggcc cggataaccc
    12001 gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt
    12061 aacccgtcag gatatccgcg ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat
    12121 tcctcttttc tttgcttacg acgtgctgca cggtcagcgc acggtgttcc cgattagcct
    12181 cggtctggcc tcgtctttta acctcgatgc agtgaaaacg gtcggacgtg tctctgctta
    12241 tgaagcggca gatgatggcc tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga
    12301 tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat acgtatctca cctcaacaat
    12361 gggtaaaacc atggtggaag cgatgcaggg taaaagcccg gcagatcgct actcggtgat
    12421 gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac
    12481 cgtcgatatg agtccgcagc gcctgtttaa tgattatatg ccgccgtaca aagcggggct
    12541 ggacgcaggc agcggcgcgg tgatggtggc gctgaactcg ctgaacggca cgccagccac
    12601 ctccgattct tggctgctga aagatgttct gcgcgaccag tggggcttta aaggcatcac
    12661 cgtttccgat cacggtgcaa tcaaagagct gattaaacat ggcacggcgg cagacccgga
    12721 agatgcggtg cgcgtggcgc tgaaatccgg aatcaacatg agtatgagcg acgagtatta
    12781 ctcgaagtat ctgcctgggt tgatcaaatc cggcaaagtg acgatggaag agctggacga
    12841 tgctgcccgc catgtactga acgttaaata tgatatggga ttgtttaacg acccatacag
    12901 ccatttgggg ccgaaagagt ctgacccggt ggataccaat gccgaaagcc gcctgcaccg
    12961 taaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac
    13021 gttaccgctg aaaaaatcgg ccaccattgc ggtggttggg ccactggcgg acagtaaacg
    13081 tgacgtgatg ggcagctggt ccgcagccgg tgttgccgat caatccgtga ccgtactgac
    13141 cgggatcaaa aatgccgtcg gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt
    13201 taccagtgac aaaggcatta tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga
    13261 tccgcgttcg ccgcaagaga tgattgatga agcggtgcag acggcgaaac aatctgatgt
    13321 ggtggtggct gtagtcggtg aagcacaggg gatggcgcac gaagcctcca gccggaccga
    13381 tatcactatt ccgcaaagcc aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc
    13441 gctggtgctg gtgctgatga acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc
    13501 tgatgcgatt ctggaaacct ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt
    13561 attgtttggc gattacaacc cgtccggcaa gctgccaatg tccttcccgc gttctgtcgg
    13621 gcagatcccg gtgtactaca gccatctgaa taccggtcgc ccgtataatg ccgacaagcc
    13681 gaacaaatac acttcgcgtt attttgatga agctaacggg gcgttgtatc cgttcggcta
    13741 tgggctgagc tacaccactt tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa
    13801 gcgtgacggc aaagtgactg ccagcgtgca ggtgacgaac accggtaagc gcgagggggc
    13861 cacggtagtg cagatgtact tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca
    13921 gctgaaaggc tttgagaaaa tcaccctgaa accgggcgaa actcagactg tcagcttccc
    13981 gatcgatatt gaggcgctga agttctggaa tcaacagatg aaatatgacg ccgagcctgg
    14041 caagttcaat gtatttatcg gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt
    14101 gctgtaatgc tttagtaagg gcgcgacgtt tgcgcccttt gtaggccgga taaggcgctc
    14161 gcgccgcatc cggcaggaaa cgccggaccg aatatgcctc ttaagtattt atctgattct
    14221 gcaactgaat ccttccgctc aagctaaccc cgccattatc aactatgctt ttctcttaat
    14281 tcgctgaaaa caacagtaaa agaatgagga aagcatcatg ccactcttaa agctctgggc
    14341 aggttcactg gttatgttgg cagccgtgag cctgccgcta caggcggctt cccccgttaa
    14401 agtcggttca aaaatcgata ccgaaggtgc gctactcggc aatatcattt tgcaggtgct
    14461 ggaaagccac ggcgtaccga cggtgaataa agtgcaactc ggaacgactc ctgtggtgcg
    14521 cggggcgatt acttccggtg aactggatat ctatccggaa tataccggca atggcgcttt
    14581 cttctttaaa gatgaaaacg atgcagcgtg gaaaaacgcg cagcaaggtt acgagaaagt
    14641 caaaaagctc gattcggagc acaacaagtt aatctggctg acgcccgcgc ctgcaaataa
    14701 cacctggact atcgccgtgc gtcaggatgt ggcagagaaa aataaactca cttcgcttgc
    14761 cgacctgagt cgttatctgc aagagggcgg caccttcaaa ctggcagcct cggcagagtt
    14821 tatcgaacgc gccgatgcgt tacccgcgtt tgaaaaagcc tatggcttta agctcggtca
    14881 ggatcagttg ctgtcactgg ctggtggcga cacggcggtg acgatcaaag ccgctgccca
    14941 gcaaacctct ggcgttaatg ctgcaatggc ttacggcact gacggcccgg tagcggcgct
    15001 ggggctgcaa accttaagcg atccgcaagg cgtgcaacct atctatgcgc ctgcaccagt
    15061 ggtgcgtgaa tcggtgttga aggagtatcc gcaaatggca cagtggctac agccagtctt
    15121 cgccagcctc gatgcaaaaa cattgcagca actgaatgcc agcattgcag ttgaaggact
    15181 ggatgccaaa aaagtggctg ccgactacct gaaacaaaaa gggtggacga agtaatttcc
    15241 cgtgacttat ctacgtatta atcctgttct ggcgctgctg ctgttgctga cggtaatcgc
    15301 agcggcgctg ccgtttatca gttacgcgcc taatcgttta gtttcgggtg aggggcgtca
    15361 tctctggcag ctgtggccgc aaacgatctg gatgctggtg ggcgttggtt gcgcctggct
    15421 gacggcctgt tttattcccg gtaaaaaagg cagcatttgt gcactcattc tggcgcaatt
    15481 cgtcttcgta ttgctggtgt ggggcgcggg aaaggcggcg acacaactgg cgcaaaatgg
    15541 cagtgcgctg gcgcgtacca gcctcggcag tggtttctgg ctggctgcgg cgctggcatt
    15601 gctggcctgt agcgatgcca tccgccgaat ctccacgcat ccgctgtggc gctggttgtt
    15661 gcatatgcag attgccatta ttccgctgtg gttgctgtac tccggcacgc ttaacgatct
    15721 ctcactaatg aaagaatacg ccaaccgtca ggatgtgttt gacgacgcgc tggcacaaca
    15781 tctgacgttg ctgtttggtg cggtgctgcc tgcgttagtg attggtgtgc cgttgggcat
    15841 ctggtgctac ttttccactg ctcggcaggg ggcaattttt tctctgctca atgtcattca
    15901 gaccgtgcct tcggtggcgc tctttggcct gttgattgcg ccgcttgccg cgctggtgac
    15961 ggcctttccg tggctgggga agctcggcat agcaggaacc ggaatgacac ccgcactgat
    16021 tgcgctggtg ctctatgcct tgctgccgct ggtgcgcggc gtggtagtcg gtttgaacca
    16081 gatcccgcgc gatgtgctgg agagcgccag agcgatgggc atgagcgggg cgcagcgatt
    16141 cctgcatgtt cagttaccac tggcgttacc ggtatttctg cgcagcctgc gggtggtgat
    16201 ggtgcaaact gtaggtatgg cggtgattgc ggcgttaatc ggcgcaggcg gttttggtgc
    16261 gctggttttc caggggctgc taagcagcgc cattgattta gtgttgctgg gggtgatccc
    16321 ggtaattgtt ctggcggtgc tgatcgatgc cctgttcgat ttgcttatcg cactgctgaa
    16381 ggtgaaacgt aatgattgaa tttagccatg tcagcaaact gtttggcgca caaaaagccg
    16441 ttaacgatct taatctcaat tttcaggaag ggagtttttc ggtgctgatt ggcacatctg
    16501 gctccggcaa atccaccacc ttgaaaatga ttaaccgcct ggtggaacat gacagcggag
    16561 agatccgctt tgccggagaa gaaattcgct cgctgccagt actggagttg cgccgccgga
    16621 tgggctatgc cattcaatct attggcctat tcccccactg gagcgtggcg caaaacatcg
    16681 ctaccgtgcc gcaattacaa aaatggtcgc gggcacggat tgacgatcgt atcgacgaat
    16741 taatgacgct actggggctg gagtcaaatt tgcgtgagcg ttatccgcat cagctttccg
    16801 gtggtcagca gcaacgtgtg ggagtggcgc gcgcactggc tgccgatccg caagtcttac
    16861 tgatggatga accttttggc gcactggacc cggtaacgcg cggcgcgttg caacaagaga
    16921 tgacgcgcat tcaccgtttg ctggggcgca ctattgtgct ggtcactcat gatattgatg
    16981 aggcgctacg gctggctgaa catctggtat tgatggatca cggtgaagtg gtgcagcagg
    17041 gcaatccgct gacgatgctg actcgtccgg cgaatgattt tgtccgccag ttttttggac
    17101 gtagcgaact gggtgtgcgt ctgctttcgt tacgtagtgt ggcggattac gtgcgtcgcg
    17161 aagaacgggc agaaggtgag gcactggcag aagagatgac gctacgcgat gcgctctccc
    17221 tgtttgtcgc gcggggttgc gaggtgctgc cggtggtgaa cacgcagggc cagccttgcg
    17281 gtacgctgca ttttcaggat ctgctggtgg aggcgtaagc gtatgaagat gttgcgcgat
    17341 ccgctgttct ggctcattgc tttgtttgtg gcactgattt tctggctgcc ttacagccag
    17401 ccgctgtttg ctgccttgtt cccacaactg ccacgacccg tttatcagca agaaagtttt
    17461 gcagctctgg cactggctca tttctggctg gtgggaattt cgagtttgtt tgcggtgatc
    17521 attggcactg gtgccggaat tgctgtcact cgcccgtggg gcgcggaatt tcgcccactg
    17581 gtggaaacta ttgccgccgt tggacagact tttccgcctg tcgcagtgct ggcgattgcc
    17641 gttccggtga tcggctttgg tctgcaacca gcgattatcg ccttgatcct ttacggtgtg
    17701 ctgcccgtcc tgcaggcgac acttgccggg ctgggagcga tcgatgccag cgtgacagaa
    17761 gttgcgaaag gtatgggaat gagtcgtggt cagcgactgc gtaaggtcga gctaccgctg
    17821 gcggctccgg tgattctggc gggcgtgcga acttcggtga ttatcaacat tggtacggcg
    17881 acgatcgcct caacggtagg ggccagcacg ctgggtacgc ccatcatcat cgggcttagc
    17941 ggatttaata ccgcgtatgt gatccagggg gcgttactgg tggcactggc ggcgatcatc
    18001 gcagaccgcc tgtttgaaag gctggtgcag gcgcttagcc agcacgcaaa ataaaggtat
    18061 aacctgcgag catgacgcca ccaattccgc cgaacgccat aaacaggaac agggcgatga
    18121 ccccaatttt agctatgcgc ataatgcact ccttatgtta acgaaaggat tgtacagtaa
    18181 agcgcatttg ttaacgaatc attaaatgcc gagtgggaaa atatcatggc cttgttcttg
    18241 ccaactggtg agttgctgct gttgggcgga ggttcgattt tcaccgcacc acaccagcaa
    18301 tgtacgacct tcgaatagtt cagggcgtag ttgattgagc gagtgggcga ggacatcaat
    18361 gcgccatcct tgttgactgg caatccagcc ctccagccac agacgggtgg tatcctgaat
    18421 attccagcca accaccagcg catctttacc ctgtttttta cgtgccgaag ccagacaaat
    18481 ggcgatgtag ttgatcagta cgccgtcgag gatcgccagc agcgcctgga gagtcggttg
    18541 ttggcattga agtcgtcggc gcagaggaat aaacagatgt gtggtgagtg tctgggcggg
    18601 gtaatcctga ccgcgctctt tgatccacgt tcgcaggcta tgcagattgc cgctttgcag
    18661 gtaggtcagt aatgtttctt gctgatcgcg ccagccgttc tgcacatcaa cattttcatt
    18721 actgagcagc attttaactt tgctgacctg cacgccgttg tcgatccagc gtttgatctc
    18781 gcggatacgg tcaatatcgg catcgttgaa cagtcgatga ccgccgtctg tccgttgcgg
    18841 tttcagcaac ccgtaacgcc tctgccacgc gcgtaacgtg acagggttaa tatcacaaag
    18901 caacgccact tcaccaattg tgtaaagcgc catcgtctca cccttgctcg cgaggtcccg
    18961 gtttaacttt agacgcagtt ttgcgaacca ggtagttttg cccgtttttt gtgcatctat
    19021 agggtgattt tatttttgcc aggcgatttt gagtgatcgt actcacgaat tctcattttt
    19081 ctgcaagagt tcaaagaaag ttaaacgcag gcaatgtatg ttacgcgttt taaagggaag
    19141 tgtggtttgc gggtatgtac gattttaatc tggtgttgct gctgcttcag cagatgtgcg
    19201 tttttttagt cattgcgtgg ttaatgagta aaacgccatt attcataccg ttaatgcagg
    19261 tcacggttcg tctgccgcat aaatttctct gctacatcgt cttttccatc ttctgcatca
    19321 tgggcacctg gtttgggttg cacattgacg attctattgc caatacccgt gcgataggcg
    19381 cggtaatggg cggcttactc ggcggtccgg tcgtcggtgg gctggttggt ctgaccggcg
    19441 gcttacatcg atattcgatg gggggcatga ccgcgctaag ttgcatgatc tcaaccatcg
    19501 ttgaaggatt gctcggcggc ctggtacaca gcatcctgat ccgtcgcggg cgcactgata
    19561 aagtctttaa ccccattacc gccggtgccg tcacgttcgt cgctgaaatg gtgcaaatgc
    19621 tgatcatcct ggcgatcgcc cgaccttatg aagatgcggt gcgtctggtg agtaatattg
    19681 ctgcgccaat gatggtcacc aataccgtcg gcgcggcgct gtttatgcgt atattgctcg
    19741 ataaacgcgc gatgtttgaa aaatacactt cggctttttc tgccactgcg ctgaaagtgg
    19801 cagcctcgac ggaaggcatt ttgcgacagg ggtttaacga agtgaacagc atgaaagtgg
    19861 ctcaggtgct gtatcaggag ctggatattg gtgcagtcgc gattaccgat cgagagaaat
    19921 tgctggcctt taccggaatt ggtgacgacc accatttacc cggcaaaccg atttcttcga
    19981 cttacacctt aaaagcgatt gaaaccggtg aagtggtcta cgctgatggc aacgaagtac
    20041 cttatcgttg ctctttgcat ccgcaatgca aactggggtc gacgctggta attccgttgc
    20101 gtggcgaaaa tcagcgggtg atgggcacca tcaaattgta tgaagccaaa aaccgtttat
    20161 tcagttcaat caaccgcacg ctgggcgagg ggattgcgca actgctttcg gcgcagatcc
    20221 tcgccgggca atatgagcgg caaaaagcga tgctcaccca gtcagagatc aaactgcttc
    20281 acgcccaggt gaacccccat tttttgttta atgcgcttaa caccattaaa gcggtgatcc
    20341 gtcgcgacag cgaacaggcc agccagctgg tgcagtatct ttccactttt ttccgcaaaa
    20401 acttaaagcg gccttcggag tttgttactc tcgccgacga aattgaacat gtgaacgctt
    20461 atctgcaaat tgaaaaggcg cgcttccagt cgcggttgca ggtcaacatt gctattccgc
    20521 aagaattatc ccagcagcaa ttgcccgcgt ttaccctgca accgatagtg gaaaacgcca
    20581 ttaaacatgg gacatcacaa ctgctggata cagggcgagt ggcaatcagc gcccgacgtg
    20641 aggggcaaca tttgatgctg gagatcgaag acaatgccgg tttgtatcaa ccggtaacca
    20701 atgccagtgg gctggggatg aatctggtgg ataagcgttt acgtgaacgg tttggcgatg
    20761 actatgggat aagcgtcgcc tgtgagcctg atagttacac ccgaataacg ttacgactac
    20821 catggaggga cgaggcatga ttaaagtctt aattgtcgat gatgaaccgt tagcacggga
    20881 gaacctgcgt gtatttttgc aggagcagag cgatattgaa atcgttggag agtgttcaaa
    20941 cgccgtggaa gggatcggcg cggtgcataa actgcgcccg gatgtgctgt ttctcgatat
    21001 ccagatgccg cgcatcagtg gtctggaaat ggtggggatg ctcgacccgg aacatcgtcc
    21061 gtatattgtt tttctcaccg cgtttgacga atacgctatt aaagcctttg aagaacatgc
    21121 ctttgattat ctgctgaagc caattgatga agcgcgactg gagaaaacgc tggcgcgttt
    21181 gcgtcaggaa cgcagcaagc aggatgtttc gctgttaccg gaaaatcaac aggcgctgaa
    21241 atttatccct tgtacggggc atagtcggat ttatttgctg caaatgaaag atgtggcatt
    21301 tgtcagcagt cggatgagcg gtgtctacgt taccagccac gaagggaaag agggctttac
    21361 cgaattgaca ttacgtaccc tggaaagtcg tacaccacta ctgcgctgcc atcgtcagta
    21421 tctggttaac ctcgcgcatt tacaggagat tcgtctggaa gataacggcc aggccgagtt
    21481 gattttgcgt aatggcttaa ccgtgccggt cagccgccgt tatctgaaaa gcttaaaaga
    21541 ggcgattggc ctgtaaaaga ctgctaaaat ggctttttgc ctcatcaaca cctgaaggcc
    21601 tcatgctaag taacgatatt ctgcgcagcg tgcgctacat tttgaaagcc aataataatg
    21661 acctggtgcg tattctggcg ctgggtaatg tcgaagccac cgcggaacag atcgccgtct
    21721 ggctacgtaa agaagacgaa gagggttttc agcgttgtcc ggacattgtt ttgtcgtcat
    21781 tcctcaatgg cctgatttat gaaaaacgcg gcaaggatga gtctgctccg gcactggagc
    21841 cggaacgtcg cattaataac aacatcgtgc tgaaaaaatt acgcatcgcg ttttcgctga
    21901 aaaccgatga cattctggct atcctcaccg aacagcagtt ccgcgtttcg atgccggaaa
    21961 ttacagcgat gatgcgcgca ccggatcata aaaacttccg cgaatgcggc gatcaatttt
    22021 tacgttattt tctgcgtgga ctggcagcgc gccagcatgt gaagaaaagc taagacgggt
    22081 atggcggcca tgcgaaacat ggccgccgac agattatttc acttctttaa aaccagcggc
    22141 tttcatcacc agttccattt gcgccatagt gatacctttt ttggcatctt cagcagaaac
    22201 gttgattcct gaaataccct gcagggcttt aaaatccact ttttccatat cgatagtcac
    22261 gttttcctgc gcgtaggtat ctgtataggt taatttttct tcaacacccg cgatgttttt
    22321 gtatttggcg cttaacggct caagtgtctt ggcagcgtct tctttggtgg ttgcaccaat
    22381 agaagcaaat tgaattttgg tttcagaaga ttgcttaagc accttgtcac ctttgtagac
    22441 ataggtaatg gcaatttcag tgccgttcag attggcgctg aatttcttcg attcttcttt
    22501 gtcaccgcag ccagcaagag agaaaaccag aacagatgca acaacgaggg aaaacagctt
    22561 attgaaagcc ttcatgtaaa actccatttt atttaatcaa gaaactggtg actctcacca
    22621 ggggctatat agaatatgcc taataccgtg acgtgagcag tccggaactg gagtagaact
    22681 cttagtaaaa agcactattt catccttgtt gctgaagcat ggggaataat tgttcgcaaa
    22741 gtaaaacacc gttattcatt gcttctaccc gtgcctcgct ttctgtatta cgaaattgtc
    22801 ccaacacatg tgccagccga taaaaaccga ccgcggagag gtcattcgcc agcaactctg
    22861 cctgactaat agcgctctgt tcctgatagc gccagccgtt atggagcagt tgaataagta
    22921 acgcctggca gcgcatcagc aactgatgag cggtagacgg cacaggcaaa acgctggcag
    22981 aaggtagcgg tgccacaggc gcagtttctg cgtccagcgc ccaggcgcgg gtttttgtca
    23041 tcatcacccg tggttccagt gtcaattgcc catcaacaaa actgacaaag ccagaaacca
    23101 gactcacggg gtcgtctgtt tgttgcaaaa gcgccgccat gcgttcaacg gcaaaaggag
    23161 aacaggcaga tgccggaagg gataacgtca ggagattatc ttcaccttcg ccgctgatta
    23221 cctgcgcatc cagcgtctgg cggctgctat cccaaccgag cgaaatacac tcagcgaccg
    23281 gcagaataaa taagttatcg acctgattaa gaggccgtat gcaggcgggg ggacgctggc
    23341 gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa cggcgcgctc aacatttgcc
    23401 aggcatcagg cgacagcggc acaacgctgc ttaagcggtt gcgggtagct aacagcagct
    23461 cgccatcggc actgcgtttt gctgcttgtg aaacaatttg cccacccgcc agtgcgccag
    23521 cctgaaaact aaacagccga cgcgtagctg ccggtgagtt ttcctgttca cttcgcggcc
    23581 aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc ggtaaaccag atgcgtaaac
    23641 cataatgctc aatatcctgc cagcaacgca tacctaaaga caccagccgc agatgatcaa
    23701 gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg ccacggcaca ggaggaactt
    23761 caccaacact gtcgcgccgg gccatctctt gtgcgcaatt taatcgactg tttaatgccg
    23821 caagctgacg taagcattct acggcatgat agtggctggc gcgagtgtgg aaggcatcaa
    23881 cgcttgcccg tagctgtcgt agtgattcac tcacccagcg ccagttgcag gcctctgccg
    23941 cctgcaatgt gcggttgaat gctgcctcgt agtgaataag cggctggctg atgccgccaa
    24001 gccataatgc ctggcttaat tgctgaacat attgacgaca cgtttggcct tcttcgctgg
    24061 caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat ctgccagatt aaatggttaa
    24121 attctgcttg ctgcgctttg gcctcgacga aggcctgcac agccagtacg acatgttcgc
    24181 aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat actgctacgg gaataaaaac
    24241 gcacatcgct catcggtaag cgggcagagg gaatttcgcc cggcgcacag aacaactcaa
    24301 tggtgatgcc tttagcgacc agcgcctgcg cgcgtttgcg ggtggcatcg ggcagggtag
    24361 ccagttcttc cagccagatt gccggatccc actcttcttc tttttccgtg ggctggacgg
    24421 tggcacaaag tcgttgataa cttaacacca gcatcacgcg atgacggcac atgccgctgg
    24481 ccccgcagct gcactgagcc tctttcagtg cctggctgtt cgccagctgg gtacggacac
    24541 cgtcactgaa ggtggcgatt aaagcgccgt tctcatggct gatttccggg acgttgccat
    24601 tttccagttc cttaagactg cgcttaacaa aaccggcatt gcttaacgcc gtcagtgcct
    24661 gcggtgtcag ttctaataat tccggacgta gtgaattcat gactgaagat tctccgcaag
    24721 ccatgatgcc agctcgcccg gcgtcatggc ggctatttgt gcgccgacat taaccagcgc
    24781 ctgggccgta tcgcggtcat agcaaggcgt tgcggtgcta tcgagcgctg ccagtcccag
    24841 cactttgatg ccgctctgga cacacttttt cacctgatgc gtcagcaatg atgatgaacc
    24901 cccttcgtaa aaatcgctca cgaggataat gacgcttttt gctggttgtt caataagttg
    24961 ccgaccatac tccacggcac tggcgatatt ggtcccgccg cccaactgta ctttcattaa
    25021 taactctacc ggatcggcaa cgtctgccgt gagatcaacc acgcttgtgt caaacgccac
    25081 cagatgggta cgaatgccgg gtaactgcca caaacaggcc gccatcaccg cagagtggat
    25141 caccgagtcc accatcgatc cgctttgatc aaccagtaag accagttgcc attgttcgct
    25201 ttggcgttta atgcggctgt taaagcgggg ggattcgata tacaacttgc cgtgttgcgg
    25261 gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa tcaaagtttc gcgccagtgg
    25321 aattaatgag cggcgacggc gatcgcggac accagaaaaa gcctgacgaa cttcctttgc
    25381 cagtcgcgcc ataatttctt caacaacctg gcgcactatc tggcgggcgg cagccagtac
    25441 ttcgggattc atcagatgtt tggtgtgcaa aacggcgcgt agcaggcttt ccgaaggctg
    25501 catacgttcc agcacgtcga gattcgtcac cacatcttca atgccgtagc gcagtacggc
    25561 atcgctttcc agtcgctcaa tcacctgttg cggaaacaga gtgtgaatac tgttgatcca
    25621 ctcaggggtg gtgagatttg agccacctaa tccaccagag cgttcaccac gctggagccg
    25681 ttcaggatcg cgcccataca gccactccag cgcgtggtct acctgccggg cgttgtcatc
    25741 cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt aatcgccagc gttgtagctc
    25801 acgggtggtc agaagatcgt tcagttcaga catggaaaac tccccagttt tgcagcgatg
    25861 ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc ctgtggtgga caatgcaacg
    25921 gcatttgcag cgccgaaacg ggaagttgcg ccagttgata atgctcaagc acctgatgcg
    25981 ccagcgtccc gcgttctcgt ggtggtagcc aggccatcgc cgcgcgtaaa tcgggcaggg
    26041 cattgataaa atcggcatca cttagttgat ttaaatgact gctgaaaccg gcgataaatg
    26101 tcggctgaca ggccagttga tggcgggcca gcgccagcaa accgtgcagc gcttcgccgg
    26161 attgtgccgg ggataactgc gccagcatcg tcagcgccgc ttcggcactg gcgttaggat
    26221 gttccagacg catcagtgcg ccatgagccg cgccgcgatc cagcgccggg gcatgaattg
    26281 cctgactgcg tcgctccagt aaggctaccg ccgcagaaag agaaatgccg gggagttgca
    26341 caccgctatg tagatcgcgc aggatatgac acaacgcttg ccagctatgc aggtgagcgt
    26401 gaaactcctt ttcatccggt ctgccgttag attcacacag ccacagcgtg cgatcgatag
    26461 ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat accgctaatt tcatccagcc
    26521 gccataaggc atatagcact tccagcgcct ggcccatttc ggcaaattga ttttcctggg
    26581 cgattaataa tgtgagttgc tccagtaatt gttgactgaa ggacgccaga cccgctaacg
    26641 ccgcctggct aagacatgtg gtgatactgc cgattccacc cgcgtccagc atatcggctt
    26701 ctaatttatg gcgtgcggct tcctgaagtg ttgcaccaaa gcatgcggcc tcaatcaatg
    26761 ccgcatgctg gctaagcggg cgggttaatt tccagtgttc ttcaccgttg cctgcaagtg
    26821 tcagtgtact tccctgctgg cgtacaatcc cagggatctc cagtattgcc agccgatgta
    26881 acacctgact ttgcgccagc ccatcggggg taaagcgatt taaggtaagc tccgccggaa
    26941 gagagattgc ggtacgttcc agttcgcagg tgacatcttt tggcagaggc ggttgtggcg
    27001 tggaaggggc aagtttaccg aatccgtcac ccgctaatgt gtctatcaac gttagcagaa
    27061 tcggatcggt atcgggatga ataacgccgc gatagctcca cggcaacggt gcattcaggg
    27121 cttctttaat caacgagcct gctatggcat ccagccagtc agtgcgtaac ggtagtgtat
    27181 gaccgcgcaa ctgtgccagt gccattgcat gcagatgtgc ggcagccata tccgcggtcg
    27241 aagcaggcaa gttgtgctgg cgcaaacggg tgagaaccgt ttttagcagt tgttcaccgg
    27301 cctgctgtaa gccccactgc cagcaccagt tttgccagac cggggcaggc ataccggaaa
    27361 ggtatcctgc cagcacatca aggcgctttt cactgtaggg cgtgagataa caacctgtaa
    27421 tggcatctgc cagcgagggc aattccggcg tgttaatgtc ctgcgggcat tcgcgccaca
    27481 tttttgccag tgccggagcg tgccagccac cgcagacgac taacacgtcg ccattatttt
    27541 gctgcaccgc ccatgcaatc cagcgagcca taaaggcttc gcgctgacga ttgagtgcat
    27601 cgccggggaa atcgccccgc agttgggcaa aatagtgcgc cagcgcagag ggtaatgcag
    27661 tttgctggct ttcatcttcg aacaagtgat cccacagggt atcgctgtta tccatgcggg
    27721 tggcacgcag cagtaaggcc tggctttcat cttgcgtatc aggcgaatcg tcctcttctt
    27781 cactctgcgc ccagcaaggc aaatcgatga agtaagtttg tgcctgaata cgacgtgcgg
    27841 cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg cgtccaggca ccacgcccgg
    27901 gggctgcacc gtcctgatac tggcaataac tgtaaatagc taccggaagc tggtgggaaa
    27961 aaaaaagttc gtctacccgg tcattaaaat cagccgggcc ttcaatcaac acgtatcgtg
    28021 gccgctggct ttcgattaac gatttcacca gccgggcgca ggccggacta tgatgccgga
    28081 tgccgacaat taacggctcg ctcatgcctc tttcctcacg gcaggcggtg gcgggcttga
    28141 taataagcct gccagtgagc ttctttatgt gtggcgacac gctgttcgaa gtaacggcgc
    28201 agacgagcgc gatcttcttc gttatctttg acgatggttc cggcaataca gtccaccagg
    28261 tcggcaggct cccccgcgcg attcgctaaa aaccaggcgc ggacgcctac ggcgtgggcc
    28321 acgttcacgg cttcagcggt ggacataatc gctgtcagtg tatccattga cgttttcttt
    28381 tcgccgttgg cacgcagatc gcggaaggtg cgtaccagta actccagtac tgcgtcaggc
    28441 actttatgcg gaataccgct atgggccagc aaacgcgccg aagcactggc gaccagttcc
    28501 agctcctggg cgaaatccat aatcggaaag acggtttcaa aatcgaagcg acgctttagc
    28561 gcggcactca tctcattgac gccgcgatcg cgggtattgg cagtggcaat aatattaaag
    28621 ccttcccgcg catagagctg actggcttca ccggtgagtt ccggcaccgt catcaccctg
    28681 tcagaaagca tacctaacag acagtcttgt acttccagcg gtgtacgcgt tatctcttca
    28741 aaacggacga ttttgccgtc gcgcattccc tggtaaagcg gggcagggac cagtgcttcc
    28801 gttgacggac catggttgat aagcagggcg tagttccagc cgtacttgat ttgatcttcg
    28861 gtagtagatg cgccgccctg gatggttaac ccggcatcgc cgctaatagc ggttgccagt
    28921 aattcagaca ggagagattt tgcggtgcca ggttccccca ccaacatcaa accgcgcccg
    28981 gtggcaagag tgactaacat gcgttcgaca gcggagggac taatcaccac tttacggcta
    29041 atattttgcg cgctatcgcc aaggataaaa gcacgggccg caggcaaact taactgccaa
    29101 ccgggcgggc aaggtgcgtt atcattttgt tttaattttg ccagttcatc ggcgtataac
    29161 acagcagcag gcggacgctg cagatgatta ttctgcggtg acatatattc atccctgaaa
    29221 tgtattaatg aaataaacga tattttacgc gcaataaatg tttggcgcat tagttgtttg
    29281 agcgaaaaat atctggaaag cagatcacag taaataaaaa aacctgctgg tgaggcaggt
    29341 tcttttattt actactttca ggcctaacac cacagcctgt gcaacatcgt gttccaacga
    29401 acgaattata accacggctg gaccccgttg caattaagat aatgagattt ataatccaga
    29461 ccgttgggac gttgcgccgg atcgaatcta tttcaagtcc tggctaccgg acgggtggtg
    29521 ctgcaggtgg tgccctgatc cggagtgcaa catggcgact caacaaacgt aaaccagtat
    29581 cgacaggcag tgtaagggta aggtgctggt gttgcgctcc ttaataa
//
LOCUS       DAMZII010000052        29180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZII010000052 DAMZII010000000
VERSION     DAMZII010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.052"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(206..1681)
                     /gene="ampG"
                     /locus_tag="POA77_002226"
     CDS             complement(206..1681)
                     /gene="ampG"
                     /locus_tag="POA77_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDI6365664.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1725..2303)
                     /locus_tag="POA77_002227"
     CDS             complement(1725..2303)
                     /locus_tag="POA77_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6365665.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2608..2925
                     /gene="bolA"
                     /locus_tag="POA77_002228"
     CDS             2608..2925
                     /gene="bolA"
                     /locus_tag="POA77_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDI6365666.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3269..4567
                     /gene="tig"
                     /locus_tag="POA77_002229"
     CDS             3269..4567
                     /gene="tig"
                     /locus_tag="POA77_002229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI6365667.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4813..5436
                     /gene="clpP"
                     /locus_tag="POA77_002230"
     CDS             4813..5436
                     /gene="clpP"
                     /locus_tag="POA77_002230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI6365668.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5562..6836
                     /gene="clpX"
                     /locus_tag="POA77_002231"
     CDS             5562..6836
                     /gene="clpX"
                     /locus_tag="POA77_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDI6365669.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            7024..9378
                     /gene="lon"
                     /locus_tag="POA77_002232"
     CDS             7024..9378
                     /gene="lon"
                     /locus_tag="POA77_002232"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDI6365670.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9587..9859
                     /gene="hupB"
                     /locus_tag="POA77_002233"
     CDS             9587..9859
                     /gene="hupB"
                     /locus_tag="POA77_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDI6365671.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10051..11922
                     /gene="ppiD"
                     /locus_tag="POA77_002234"
     CDS             10051..11922
                     /gene="ppiD"
                     /locus_tag="POA77_002234"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6365672.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAQQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12073..12444
                     /locus_tag="POA77_002235"
     CDS             12073..12444
                     /locus_tag="POA77_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDI6365673.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12538..12936
                     /gene="fadM"
                     /locus_tag="POA77_002236"
     CDS             12538..12936
                     /gene="fadM"
                     /locus_tag="POA77_002236"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDI6365674.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12988..13683)
                     /gene="queC"
                     /locus_tag="POA77_002237"
     CDS             complement(12988..13683)
                     /gene="queC"
                     /locus_tag="POA77_002237"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI6365675.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARARKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13748..15448)
                     /locus_tag="POA77_002238"
     CDS             complement(13748..15448)
                     /locus_tag="POA77_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDI6365676.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15548..16366
                     /gene="cof"
                     /locus_tag="POA77_002239"
     CDS             15548..16366
                     /gene="cof"
                     /locus_tag="POA77_002239"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDI6365677.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16519..16977
                     /gene="decR"
                     /locus_tag="POA77_002240"
     CDS             16519..16977
                     /gene="decR"
                     /locus_tag="POA77_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDI6365678.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            17007..18779
                     /locus_tag="POA77_002241"
     CDS             17007..18779
                     /locus_tag="POA77_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI6365679.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            18772..20553
                     /locus_tag="POA77_002242"
     CDS             18772..20553
                     /locus_tag="POA77_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI6365680.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDVSEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYLQMYQLQLAGEELAASVREEESLSA"
     gene            20734..21072
                     /gene="glnK"
                     /locus_tag="POA77_002243"
     CDS             20734..21072
                     /gene="glnK"
                     /locus_tag="POA77_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDI6365681.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21102..22388
                     /gene="amtB"
                     /locus_tag="POA77_002244"
     CDS             21102..22388
                     /gene="amtB"
                     /locus_tag="POA77_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDI6365682.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22437..23297)
                     /gene="tesB"
                     /locus_tag="POA77_002245"
     CDS             complement(22437..23297)
                     /gene="tesB"
                     /locus_tag="POA77_002245"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDI6365683.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23515..24087
                     /locus_tag="POA77_002246"
     CDS             23515..24087
                     /locus_tag="POA77_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDI6365684.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24118..24429)
                     /locus_tag="POA77_002247"
     CDS             complement(24118..24429)
                     /locus_tag="POA77_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDI6365685.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24592..24688
                     /gene="ffs"
                     /locus_tag="POA77_002248"
     ncRNA           24592..24688
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="POA77_002248"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24808..25161
                     /locus_tag="POA77_002249"
     CDS             24808..25161
                     /locus_tag="POA77_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDI6365686.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25203..26753)
                     /gene="pdeB"
                     /locus_tag="POA77_002250"
     CDS             complement(25203..26753)
                     /gene="pdeB"
                     /locus_tag="POA77_002250"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDI6365687.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26917..27387)
                     /locus_tag="POA77_002251"
     CDS             complement(26917..27387)
                     /locus_tag="POA77_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDI6365688.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLALAESTS"
     gene            complement(27503..28054)
                     /gene="maa"
                     /locus_tag="POA77_002252"
     CDS             complement(27503..28054)
                     /gene="maa"
                     /locus_tag="POA77_002252"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDI6365689.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(28226..28444)
                     /gene="hha"
                     /locus_tag="POA77_002253"
     CDS             complement(28226..28444)
                     /gene="hha"
                     /locus_tag="POA77_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDI6365690.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28470..28844)
                     /gene="tomB"
                     /locus_tag="POA77_002254"
     CDS             complement(28470..28844)
                     /gene="tomB"
                     /locus_tag="POA77_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDI6365691.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7343 a         7001 c         7865 g         6971 t
ORIGIN      
        1 aattatcatt atggtgacaa ttgggtgggg gaaaataaag actcattagc tgaatcgtgt
       61 aagaaaaaat agaattcata aattgcgtca ataatttcca aacgctaagc cgcacaaaag
      121 aacaaatatt attaagcgat actgatgttt atttagccgt aataattacg gcgagtgatt
      181 actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat
      241 ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag
      301 caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca
      361 cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat
      421 attcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa
      481 ccagcagcaa aataagcccc ggtacggcag cggcgacgga gaatagatag aatgtcgacc
      541 agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc cctacagcgg
      601 aaagcgctga gagcagggca aattgagttg cggaaaatga cttattacac agcgtcatta
      661 acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg
      721 cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg
      781 caccttgtaa aatgccgaaa atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa
      841 tcccaccgta caatgcgccc acaatggtcg ccagtaagcc aagcgttttg ttaaccacgc
      901 ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg
      961 cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc
     1021 gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa
     1081 tggtgtcggt cggttctggt gcaagcaacg tcgcgataat acaagggatc aacagcgccg
     1141 ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac agggctaagc
     1201 cgccggaaac cagcatccct aaacggtaac ccagcacgct gattgccgcg cccgcaccac
     1261 gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggccg
     1321 agcaaaaagc gatcaccacc gccagtgccg ccatccagcg gagttgggtg ccaggttcga
     1381 gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc
     1441 gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa
     1501 aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga
     1561 ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac
     1621 ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca
     1681 tgggcgtcta caggcccggt ttgcaccggg ccagagggca gaaattaacg cgcgttctgc
     1741 ttgataaatt cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca
     1801 ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag
     1861 ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata
     1921 tccgctttgg tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg
     1981 ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc
     2041 atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta
     2101 acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca
     2161 ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg
     2221 gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac
     2281 gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa
     2341 aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc
     2401 atcatgttga cgaaataatc gcccctggca aaagaaacac tgatgcgagg cctgtgtttc
     2461 aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat
     2521 ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca
     2581 tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta
     2641 agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc
     2701 ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa
     2761 cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc
     2821 gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc
     2881 gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac
     2941 tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt
     3001 tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg
     3061 tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat
     3121 aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat
     3181 tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc
     3241 gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct
     3301 tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga
     3361 gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat
     3421 gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat
     3481 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac
     3541 ttatgttccg ggcgaataca agctgggtga agacttcact tactctgtag agtttgaagt
     3601 ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga
     3661 agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg
     3721 gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc
     3781 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca
     3841 gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt
     3901 caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc
     3961 gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga
     4021 attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg
     4081 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat
     4141 cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgattg acagcgaaat
     4201 cgacgttctg cgtcgtcagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga
     4261 actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct
     4321 gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat
     4381 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa
     4441 caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt
     4501 actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca
     4561 ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg
     4621 tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
     4681 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
     4741 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
     4801 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
     4861 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
     4921 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
     4981 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
     5041 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
     5101 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
     5161 ttgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
     5221 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
     5281 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
     5341 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
     5401 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
     5461 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
     5521 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
     5581 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
     5641 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
     5701 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
     5761 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
     5821 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
     5881 gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
     5941 tgctggctga aacgctggcg cgcctgctgg acgtcccgtt caccatggcc gacgcaacca
     6001 cgctgaccga agccggttat gtgggcgaag acgttgaaaa catcattcag aagctgttgc
     6061 agaagtgcga ttacgacgta cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg
     6121 acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg
     6181 tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg
     6241 ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt
     6301 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
     6361 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
     6421 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
     6481 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
     6541 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
     6601 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
     6661 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
     6721 tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
     6781 gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa
     6841 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
     6901 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
     6961 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
     7021 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
     7081 gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt
     7141 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
     7201 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
     7261 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
     7321 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
     7381 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
     7441 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
     7501 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac
     7561 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
     7621 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
     7681 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
     7741 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
     7801 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
     7861 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
     7921 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
     7981 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
     8041 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
     8101 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
     8161 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
     8221 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
     8281 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
     8341 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
     8401 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
     8461 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
     8521 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
     8581 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
     8641 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
     8701 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
     8761 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc
     8821 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
     8881 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
     8941 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
     9001 gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt
     9061 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
     9121 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
     9181 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
     9241 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
     9301 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag
     9361 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
     9421 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
     9481 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
     9541 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
     9601 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
     9661 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
     9721 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
     9781 cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact
     9841 gaaagacgcg gtaaactaag cgttgtcccc ggtggggatg tgacgaagtt caagggcgca
     9901 tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa
     9961 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    10021 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    10081 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    10141 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    10201 agccgtgggc agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg
    10261 ggcgatcaat actccgagct ggcagcgaat gaaggctata tgaaaaccct gcgtcaacag
    10321 gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg
    10381 ggtatcagtg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    10441 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    10501 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc
    10561 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    10621 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    10681 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    10741 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    10801 gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc
    10861 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    10921 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt
    10981 aacggcggcg atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct
    11041 ggtctgaaag aaaaagggca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    11101 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    11161 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa
    11221 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgctggc
    11281 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    11341 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    11401 atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag
    11461 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcattggtt
    11521 cagcacaaca aagctcaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    11581 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    11641 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    11701 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    11761 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    11821 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    11881 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    11941 aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    12001 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    12061 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt
    12121 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac aacggtagaa
    12181 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    12241 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    12301 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    12361 tttaaaacgg tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    12421 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    12481 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    12541 caaacacaaa tcaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc
    12601 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    12661 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    12721 cgcccagcgg tattaagtga cctgttaact attaccagtc agttgcagca attaaacggt
    12781 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    12841 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    12901 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    12961 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    13021 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    13081 cgcacaatga ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg
    13141 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca
    13201 catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag
    13261 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc
    13321 ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    13381 aatattacgc cctgggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg
    13441 caccggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc
    13501 aagaacctta cgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    13561 tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    13621 tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt
    13681 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    13741 cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    13801 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    13861 gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc
    13921 ggttttcctc atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg
    13981 ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat
    14041 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg
    14101 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    14161 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca
    14221 gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg ccagtacgga atagtccagc
    14281 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    14341 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    14401 ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc
    14461 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctgtttccca
    14521 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    14581 gcaaatggta ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc
    14641 gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    14701 ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat
    14761 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    14821 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    14881 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    14941 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    15001 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    15061 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    15121 gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc acatcttgct
    15181 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    15241 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    15301 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    15361 ccagttcgct gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat
    15421 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    15481 ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc acagagtgga
    15541 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    15601 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    15661 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    15721 ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    15781 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    15841 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    15901 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    15961 gccactcggc agagtcacca agatctgctt ctgtggcggt cacgacgatc ttacacgctt
    16021 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    16081 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    16141 acatttaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    16201 aatgttaggc agcgtcggta gcgggtttat tatgggcaat gcgatgccgc aactgcgcgc
    16261 ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct ctcactattt
    16321 gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag atcccttcca
    16381 gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    16441 aaaccgcaca gaataaattg tcgtgatttc acctttaaaa tagaattaaa agagaaaaaa
    16501 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
    16561 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct
    16621 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    16681 ctggatccgg aaaaaatagg cctcggcctg accgcttttg tgctgataaa aacgcaacat
    16741 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    16801 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    16861 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    16921 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    16981 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    17041 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    17101 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    17161 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
    17221 tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    17281 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    17341 gcacgggtga cctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgccgccg
    17401 gagaaggggt gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga
    17461 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
    17521 cgatcatgat taagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg
    17581 cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
    17641 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    17701 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgacaatc tatatcgcga
    17761 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    17821 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    17881 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
    17941 gtgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    18001 gtggcgaact tgatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    18061 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    18121 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    18181 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    18241 tggcggtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt
    18301 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    18361 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga agtgggcgag cgtggcgtga
    18421 tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg agcgttatta gtcaacgcgg
    18481 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
    18541 tgcataacct gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt
    18601 cggcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
    18661 gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    18721 aactggaggc ggcgctcgac gacgttccgg aaattcgcga ggaggccgtc gatgcgtagt
    18781 tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcggaaa
    18841 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg
    18901 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg
    18961 gttgcagggc tggcggcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
    19021 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    19081 gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    19141 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    19201 gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcaat gttcagcctc
    19261 gactggcgaa tggcgctggt ggcgataatg attttcccgg tagtgctggt ggtaatggtg
    19321 atatatcaac gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    19381 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag
    19441 gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc gaggatgcaa
    19501 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    19561 ctttgtggct tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
    19621 ttatatgcgt ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
    19681 cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    19741 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagcggcac catcgaagtc
    19801 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    19861 gtgccttcgc gtaactttgt ggcgctggtc gggcataccg gcagtggcaa aagcaccctc
    19921 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    19981 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    20041 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tgggacgtga tgtctccgaa
    20101 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg cagcatgagt
    20161 gatggtattt atacgccgct gggcgagcag gggaataatc tctccgtcgg gcaaaagcaa
    20221 ctgctggcac tggcacgcgt gttggtggag acgccgcaaa tcctgatcct tgatgaggca
    20281 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggcggtgcgt
    20341 gaacatacca cgctggtggt gattgctcac cgcttatcaa ctattgttga tgccgacacc
    20401 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    20461 gcccagggac gctacttgca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    20521 agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    20581 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    20641 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    20701 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
    20761 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    20821 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    20881 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
    20941 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    21001 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    21061 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    21121 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    21181 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    21241 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    21301 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    21361 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa
    21421 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
    21481 gctcgtttgc ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct
    21541 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    21601 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
    21661 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    21721 gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta
    21781 ctgccattct ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    21841 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    21901 ggatcttcgg tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg
    21961 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt
    22021 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct
    22081 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    22141 gctgtatcat gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag
    22201 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    22261 ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg
    22321 taccggaaga gcaggagcga gaagggctgg atgtcaacag tcacggcgag aatgcctata
    22381 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    22441 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagtacgcc gtcctgggta
    22501 taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg aggtgctctc cacgctatac
    22561 agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc aatggtggca
    22621 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    22681 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    22741 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    22801 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttaagcact
    22861 ggcggcagca ggtgcgccag cgattgggcg atttgcgttt ccgaaggcag gccatcaggc
    22921 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc ctggaaagag
    22981 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    23041 ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact atcgccaggg
    23101 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt ctcttttgca
    23161 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    23221 ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt cagtaaattt
    23281 tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag ccaaagtgag
    23341 ttgagtataa cgcaaatttg ctactggtcc gatgggtgca atggtctgaa ttacgggcta
    23401 attacaggca gaaatgcgtg atgtgtgcca cacttgttga cgttactatt ttgttaacca
    23461 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa atgaatgaaa
    23521 ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    23581 agcgcggata ttcagacgcc agccccggct gcaaatacgt ctatttcagc aacacaacaa
    23641 cctgctatcc agcaaccgaa tgtctccggt accgtctgga tccggcagaa agtcgcactg
    23701 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcaccgtca
    23761 aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt
    23821 ctgccattta atccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    23881 accgtgaatg acaaactggt atttatcacc gataccgttc agccggtgat caaccagggc
    23941 ggaactaaag ccgacctgac attggtgccg gtacagcaaa ccgccgtgcc ggttcaggcc
    24001 agcggtggtg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    24061 gcagttccag ctcctacgca atattaagcg acgttaaccc tctccgcccg gagagggtta
    24121 gtagttccag cgataacgct gcaagtcgat ttgcccgctt cccgatacca tcacaccttc
    24181 tgccagtaat gcctgtcgct gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg
    24241 gcgattaacc acccggtgcc agggtaaggt gctgccttca gggagacgct ttaacacacc
    24301 gcccacctgg cgcgcggcgc ggggcgatcc cgccagtttc gccacatcac cgtaagtggt
    24361 gacatagcct tcgggaatag cggcgacgat ttgccagacg cgttggggaa atgaatcttc
    24421 tttttccatc ttttcttcct gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg
    24481 aagtcgcatt gcgcaagaaa ccagcatctg gcacgcgatg ggttgcaatt agccggggca
    24541 gcagtgataa tgcgcctgtg cgttggttct caacgctctc aatgggggct ctgttggttc
    24601 tcccgcaacg ctactctgtt taccaggtca ggtccggaag gaagcagcca aggcagatga
    24661 cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt tctgcctccc accgtttcgt
    24721 caaaaaaccc caacatggct aaactttaac cacaactgac gtcgcaagaa ttgtctggct
    24781 gcgcagtacg cttcggaggt atgtctgatg aagtatgttg atggttttgt ggttgccgtt
    24841 cctgccgata aaaaggatgc ctatcgggaa atggccgcta aggctgcgcc attgtttaaa
    24901 gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg
    24961 accgattttc gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt
    25021 gaataccctt caaaagaggt ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg
    25081 atgaaagagt tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggcggattc
    25141 gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa
    25201 attcacaaat gttgctcggc ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag
    25261 agccagccct gaccgtagtg cacgccatgc tggcgtagcc actcttcttg tttactggtt
    25321 tcgattccct ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc
    25381 gtgacatttt tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca
    25441 tccagatcct gtaaataact taaacttgaa tatcccgtac caaaatcatc aagataaatt
    25501 tcatggcccg cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac
    25561 tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa cctgatagtg attgatcatt
    25621 tcacgcagca attgcgggat tttttccgag gtgagcacgg tggattcaag attgatcgaa
    25681 atatgctgct gtggatgctg acgcagccag tcgcccatat cttcaaagac gcttcttata
    25741 atcagtagcg tcaatggctc agaaaggccc gtttgctgtg ccagcggaat aaaactatct
    25801 ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt
    25861 ttgccattgg ctaaggagac aatcggctga tagtgcacgc aaatatcacg attttcgata
    25921 gcatcctgca gccgatgatg cggtgactgg atacggcgca aaatacgcag cacaaacatc
    25981 gctgccagca ggccaatcac caacccgagc ggtaaccaaa taaagacttg ccgatgccag
    26041 cctttctgca acattttcgt tgaagcccag gtgatgatcg aaatattcat ctccgggaag
    26101 ggcaggatat cgtaaatgat tccattattt tcgatatgct caccgggtgt tttttgtagc
    26161 ctggtaataa ttccctgagc aatttcatcg ctgctggtta tgacaacgtt atgggcattg
    26221 ccaataatgg cggcatcaat ttgccatgag ctatagggaa tgacatcaat aaaggaagcg
    26281 gggtcgatca tgacgacata atgtgccgtt cccatggcga ccatgtaacg gataatgcct
    26341 aaatcgttat gcgatgttaa ccagacacga taaccatctt tcgaaatttt acctggctcg
    26401 gggaaggtat cgggcggact ttcatgctcc agagacgaac actggggaac gttgttatcg
    26461 atataaacca cttcctgaat atagcgataa ctgtaagata cccgacgcat ttccatgaga
    26521 tgggcttcgc tacaggcagc gccttgccat ctttccagct cttgcagcgc atctttccct
    26581 tgtgtcgcca ccttattggc ccgaatagcg acgcgggagg aataggtatc cagctcttca
    26641 ataaacgatg tttctacctg ctgatgggcc agccagatgc ttaagccgac aggcagcaat
    26701 actgaaagaa tcagtactcc cgaaataagg ccgaccagat gtcgtgttct caccagcata
    26761 tccttattaa cacagtgtag ggaaagtata tctgattgtg gaaaaaggct ggcaggagat
    26821 aaggaggtag agctggtatt tcatgaagtg atgggtggaa acgatcattg tcatttgacc
    26881 aggcgcagag ctttacgcct ggtatgtaac cctaacttaa gatgttgatt cggcgagagc
    26941 gagggtaaaa atatcgtaaa aagacagttc acctttacgg acgatcattt tttgcagttg
    27001 ttccttatga acatccgcga cacgcggaga gttcaagatg gcttcaacca gcgcggcagg
    27061 tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat
    27121 atcttcataa cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa
    27181 taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca tcaccgccag
    27241 ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac ggataaaact
    27301 gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc
    27361 ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg
    27421 gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatttac cacaattttg
    27481 cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga ttaccgccca
    27541 cgacaacgtt gttcgggaca tcttttgtga caactgcacc tgaggctacc acgacgttat
    27601 caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg ttattaccga
    27661 tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg atgggatgtg
    27721 ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg cgaatagggc
    27781 agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat
    27841 agccatagtc acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaatagat
    27901 cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa
    27961 taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag cgatacaact
    28021 caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc
    28081 ggcaacacag taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgataa
    28141 agatcacata gggtatgtct tcatggcgaa aaagtataaa attcttaata aacagccggt
    28201 tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag gaagggatct
    28261 tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg atctgcggct gagtaaaata
    28321 ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc tccagcgtgt
    28381 caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa ggtttttcgg
    28441 acatacttct acccatggtt gtaataattc taacaagata aactcgcagg attctctttc
    28501 gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt ctgaagatcc
    28561 tgcatattaa taccataact actgaacaac ataaaggtgt catccagata ttcgtcgatc
    28621 tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc gaaggtcgca
    28681 atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc gttaacccag
    28741 ccatgattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc acagagaaac
    28801 ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg tccccttctt
    28861 agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct ggacttgccc
    28921 tctcgtttgt tagcttaact ataagccact ctttgcaggt tttcatcgca ttattacgaa
    28981 aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc actaacccgc
    29041 agcaggtaaa agcagtctgc tcgaactgtt cataacattt caggattatc gccagatgaa
    29101 attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag gaatgaataa
    29161 agaattaccg caaataatta
//
LOCUS       DAMZII010000053        29068 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZII010000053 DAMZII010000000
VERSION     DAMZII010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.053"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            313..1179
                     /gene="folD"
                     /locus_tag="POA77_002255"
     CDS             313..1179
                     /gene="folD"
                     /locus_tag="POA77_002255"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI6365692.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1181..1393
                     /gene="ybcJ"
                     /locus_tag="POA77_002256"
     CDS             1181..1393
                     /gene="ybcJ"
                     /locus_tag="POA77_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDI6365693.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1501..2022
                     /locus_tag="POA77_002257"
     CDS             1501..2022
                     /locus_tag="POA77_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6365694.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2058..3443)
                     /gene="cysS"
                     /locus_tag="POA77_002258"
     CDS             complement(2058..3443)
                     /gene="cysS"
                     /locus_tag="POA77_002258"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI6365695.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3617..4111
                     /gene="ppiB"
                     /locus_tag="POA77_002259"
     CDS             3617..4111
                     /gene="ppiB"
                     /locus_tag="POA77_002259"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDI6365696.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4114..4836
                     /gene="lpxH"
                     /locus_tag="POA77_002260"
     CDS             4114..4836
                     /gene="lpxH"
                     /locus_tag="POA77_002260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDI6365697.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4954..5463
                     /gene="purE"
                     /locus_tag="POA77_002261"
     CDS             4954..5463
                     /gene="purE"
                     /locus_tag="POA77_002261"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI6365698.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5460..6527
                     /gene="purK"
                     /locus_tag="POA77_002262"
     CDS             5460..6527
                     /gene="purK"
                     /locus_tag="POA77_002262"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI6365699.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(6722..7615)
                     /locus_tag="POA77_002263"
     CDS             complement(6722..7615)
                     /locus_tag="POA77_002263"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6365700.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7612..8427)
                     /locus_tag="POA77_002264"
     CDS             complement(7612..8427)
                     /locus_tag="POA77_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6365701.1"
                     /translation="MTIIHPLLASCSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8438..9697)
                     /locus_tag="POA77_002265"
     CDS             complement(8438..9697)
                     /locus_tag="POA77_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6365702.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9707..11374)
                     /locus_tag="POA77_002266"
     CDS             complement(9707..11374)
                     /locus_tag="POA77_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6365703.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11691..12740
                     /gene="allD"
                     /locus_tag="POA77_002267"
     CDS             11691..12740
                     /gene="allD"
                     /locus_tag="POA77_002267"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDI6365704.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12762..13997
                     /gene="allC"
                     /locus_tag="POA77_002268"
     CDS             12762..13997
                     /gene="allC"
                     /locus_tag="POA77_002268"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDI6365705.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14008..14793
                     /gene="allE"
                     /locus_tag="POA77_002269"
     CDS             14008..14793
                     /gene="allE"
                     /locus_tag="POA77_002269"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDI6365706.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14922..16067)
                     /gene="glxK"
                     /locus_tag="POA77_002270"
     CDS             complement(14922..16067)
                     /gene="glxK"
                     /locus_tag="POA77_002270"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDI6365707.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16089..17390)
                     /locus_tag="POA77_002271"
     CDS             complement(16089..17390)
                     /locus_tag="POA77_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDI6365708.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTVLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17447..18808)
                     /gene="allB"
                     /locus_tag="POA77_002272"
     CDS             complement(17447..18808)
                     /gene="allB"
                     /locus_tag="POA77_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDI6365709.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILHGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18868..20322)
                     /locus_tag="POA77_002273"
     CDS             complement(18868..20322)
                     /locus_tag="POA77_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDI6365710.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTVEKTGEQKTIG"
     gene            complement(20492..21370)
                     /gene="glxR"
                     /locus_tag="POA77_002274"
     CDS             complement(20492..21370)
                     /gene="glxR"
                     /locus_tag="POA77_002274"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI6365711.1"
                     /translation="MKLGFIGLGIMGTPMAVNLARAGHQLHVTTIGPVAEELLALGAV
                     NVETARQVTESSDIIFIMVPDTPQVEEVLFGENGCAKTSLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21469..22245)
                     /gene="hyi"
                     /locus_tag="POA77_002275"
     CDS             complement(21469..22245)
                     /gene="hyi"
                     /locus_tag="POA77_002275"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDI6365712.1"
                     /translation="MLRFSANLSMLFQEYDFLERFDKAAQCGFRAVEFMFPYDYDIEV
                     LQHKLSSNNLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAVAIRYARALGNKKI
                     NCLVGKTPVGVSAEQVHTTLVENLRYAANMLAKEGILLLIEPLNHFDMPGFHLTGTQQ
                     ALALIADIGSNNIKIQYDIYHMQRMEGELAQTMTTWANKIGHLQIADNPRRGEPGTGE
                     INYDFLFDLIEKSDYDGWVGCEYKPLTTTEAGLSWINPYR"
     gene            complement(22258..24039)
                     /gene="gcl"
                     /locus_tag="POA77_002276"
     CDS             complement(22258..24039)
                     /gene="gcl"
                     /locus_tag="POA77_002276"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDI6365713.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMNQYRVPVVVEIILERVTNISMGNE
                     LDNVMEFEDIADNAADAPTETCFMQYK"
     gene            complement(24129..24944)
                     /gene="allR"
                     /locus_tag="POA77_002277"
     CDS             complement(24129..24944)
                     /gene="allR"
                     /locus_tag="POA77_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDI6365714.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25022..25504)
                     /gene="allA"
                     /locus_tag="POA77_002278"
     CDS             complement(25022..25504)
                     /gene="allA"
                     /locus_tag="POA77_002278"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDI6365715.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25734..26660
                     /gene="allS"
                     /locus_tag="POA77_002279"
     CDS             25734..26660
                     /gene="allS"
                     /locus_tag="POA77_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDI6365716.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26729..27823
                     /gene="mnmH"
                     /locus_tag="POA77_002280"
     CDS             26729..27823
                     /gene="mnmH"
                     /locus_tag="POA77_002280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDI6365717.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27939..28303
                     /locus_tag="POA77_002281"
                     /pseudo
     CDS             27939..28303
                     /locus_tag="POA77_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28824..28997)
                     /locus_tag="POA77_002282"
     CDS             complement(28824..28997)
                     /locus_tag="POA77_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365718.1"
                     /translation="MAKIVSENDPDTKKVWCKYGKIPGQGDSVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
BASE COUNT         7187 a         7842 c         7205 g         6834 t
ORIGIN      
        1 gcaaaatata ttcaacaaaa atatagacct gaagcggtaa attaccaggc tgaaaattct
       61 ttttatattg tcaggtattt cttaaattat cttaatcctt aaacaaggaa ataaatcagt
      121 tccagattta caacgccatc atggacgaaa aatgaagctt tcaatctcag cgacctcagc
      181 gacggtgcgc ctaaccttcg caagaggtcg cttcacgcga taaatctgaa acgaaacctg
      241 acagcgcgcc ccgcttctga caaaatagac gcatcccctt cgacctacgt aacagatgga
      301 atcctctctc tgatggcagc aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc
      361 tctgaagttg ctcaaaaagt tcaggcgcgt attgcagccg gactgcgggc accaggactg
      421 gccgttgtgc tggtgggtag taaccctgca tcgcaaattt atgtcgcaag caaacgcaag
      481 gcttgtgaag aagtcgggtt cgtctcccgc tcttatgacc tcccggaaac caccagcgaa
      541 gcggagctgc tggagcttat cgatacgctg aatgccgaca acaccatcga tggcattctg
      601 gttcaactgc cgttaccggc gggtattgat aacgtcaaag tgctggaacg tattcatccg
      661 gacaaagacg tggacggttt ccatccttac aacgtcggtc gtctgtgcca gcgcgcgccg
      721 cgtctgcgtc cctgcacccc gcgcggtatc gtcacgctgc ttgagcgtta caacattgat
      781 accttcggcc tcaacgccgt ggtgattggc gcatcgaata tcgtcggtcg cccaatgagc
      841 atggaactgt tgctcgcagg ttgcaccact accgtgactc accgcttcac taaaaatctg
      901 cgtcatcacg tagaaaatgc cgatctgttg atcgttgccg ttggcaagcc aggctttatt
      961 cccggtgact ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa tcgtctggaa
     1021 aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt
     1081 acgcctgttc ccggcggcgt tggcccgatg acggttgcca cgctgattga aaacacgcta
     1141 caggcgtgcg ttgaatatca tgatccacag gatgagtaac atggcgacat tttctttagg
     1201 taaacatccg cacgttgagc tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg
     1261 cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggtg cggttgaaac
     1321 gcgcaaacgc tgcaaaatcg tcgccggtca gacagtgagt tttgcaggtc acagcgtaca
     1381 ggttgttgcc tgatcccttt cccggcctgt atcaacaggc cggttctctt ttcagacttt
     1441 tgccctctac gccccgttca tccatgctga tagcatcgac attttgccga agcgaccatc
     1501 atgccaaccg ttattacgca cgccgccgtt cccctatgca ttggtttagg gttgggttcg
     1561 aaagtcatcc ctccgcgtct gttatttgcc ggaatcatcc tggcgatgct gcccgacgcc
     1621 gacgttttgt cgtttaaatt tggcgttgct tacggcaatg tttttggtca tcgcgggttt
     1681 acccattcgc tggtgtttgc gtttgttgtc ccgctgttat gtgtgctgat tggacgccga
     1741 tggttcaggg ctgggctgat tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac
     1801 agcttgctgg attcggtaac cactggcggt aagggcgttg gctggctgtg gccgtggtca
     1861 gatgaacgct ttttcgctcc ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt
     1921 tacaccacgc cgtacgggca tcaggtgata atttctgaat tgatgtgggt gtggttgccg
     1981 gggatggtgc ttatgggaat gttgtggtgg cgcaggcgat aaccggatgc gaaaactcgc
     2041 atccggcaac agcgcaatta cttacgacgc caggtggtcc cttgcgggcc atcttccagc
     2101 acgatcccca tctcgttaag acggtcacgc gccgcatctg ccgccgccca gtctttcgct
     2161 ttacgggcat ccagacgctg ttgaattaac gcttcaatct cagccacttc gctgtcgtct
     2221 gcctgcgcgc cgctttgcag gaacgcttcc ggttcttgct ccagcaggcc caatacagcg
     2281 gaaagtttac gcaggtgaga tgccattgca ttcgccgctg ccatatcttc tgctttcaga
     2341 cggtttactt cacgcgccat atcaaacagt acggaatagg cttccggggt gttgaaatcg
     2401 tcgtccatcg cttcaataaa gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt
     2461 ttatctgtgc cgcgcagcgc agtgtagaga cgctccagcg ccgcacgcgc ctgcttcagg
     2521 ttctcttcgc tgtagttcaa ctggctgcga tagtggcccg acatcaggaa gtaacgcacg
     2581 gtttccgcgt cgtagtattt cagcacatcg cgcacggtaa agaagttacc cagcgatttg
     2641 gacatcttct cgcggtcaac catcaccatc cccgagtgca tccagtagtt cacatactgg
     2701 ccatcatggg cacaggtgga ctgcgcgatt tcgttctcgt ggtgcgggaa catcaggtct
     2761 gaaccgccgc cgtggatatc aaagtggtta cccagctgct tgcagttcat tgccgaacat
     2821 tcaatgtgcc agcctggacg gcccgcgccc cacggagacg gccagctcgg ttcgccctct
     2881 ttcgacatct tccacagaac gaagtccatc gggttgcgtt tgtcgtcgac cacgtcaacg
     2941 cgcgcacctg cctgcagctg gtcgagatcc tgacgcgaca gcacgccata tgtcggatcg
     3001 gtcgggacgt cgaacatcac gtcgccgttg tccgccacat aagcgtgacc tttggcgatc
     3061 agttgttcag tgagttcaat aatttctgcg atatggtgcg tcgcgcgcgg ctccatatcc
     3121 gggcgcagaa tgttcaaagc atcaaaatct ttgtgcattt cggcgatcat gcgatccacc
     3181 agcgccacaa agctttcgcc attttcattg gcgcgtttga tgattttgtc gtcgatatcg
     3241 gtaatgttgc gcacatactt cagtttatag ccgaggaaac gcagatagcg cgcaaccacg
     3301 tcaaaagcaa caaaggtacg cccgtgaccg atatgacaga gatcgtaaac ggtgattcca
     3361 cacacataca tgccgacttc cccggcgtga ataggcttaa attcctcttt ttggcgtgtc
     3421 agagtattga agatttttag catcgaagat tccgtttaga catgtgtggg taattgagtt
     3481 gcgtataata cccatatttc ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg
     3541 cggttatgct ataacaccac cctatatatg acccgaactg ggttgaagca ccaatcaaac
     3601 ggaacaggat gcaaaaatgg ttactttcca caccaatcac ggcgatattg tcatcaaaac
     3661 ttttgacgat aaagcacctg aaacagttaa aaacttcctg gactactgcc gcgaaggttt
     3721 ttacaacaac accattttcc accgtgttat caacggcttt atgattcagg gcggcggttt
     3781 tgaaccgggc atgaaacaaa aagccaccaa agaaccgatc aaaaacgaag ccaacaacgg
     3841 tctgaaaaat acccgtggta cgctggcaat ggcccgtact caggctccgc actctgcaac
     3901 tgcacagttc ttcatcaacg tggttgataa cgacttcctg aacttctctg gcgaaagcct
     3961 gcaaggttgg ggctactgcg tgtttgctga agtggttgaa ggcatggacg tggtagacaa
     4021 aatcaaaggt gttgcaaccg gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt
     4081 tatcattgaa agcgtgaccg ttagcgagta atcgtggcga cactctttat tgcagatctt
     4141 catctctgcg tggaagaacc ggcgatcacc gccggttttc tgcgtttttt agcgggggaa
     4201 gcccgcaagg ccgacgcgct gtatattctt ggcgatctgt ttgaagcatg gattggcgac
     4261 gacgatccca acccactcca tcgccagatg gcggcagcga tcaaagcggt gtccgattcc
     4321 ggcgttccct gttatttcat tcatggcaac cgtgattttc tgctcggcaa acgctttgcc
     4381 cgtgaaagcg gcatgacatt attgccggaa gaaaaggtgc tcgaacttta tggtcgccgg
     4441 gtgttgatta tgcatggcga cacgctgtgc accgatgacg cgggttatca ggcttttcgc
     4501 gccaaagtcc acaaaccctg gctgcaaatg ctattcctcg ccctgccgtt gtttgtgcgc
     4561 aaacgcattg ccgcgcgaat gcgagcgaac agtaaagaag ccaacagcag taaatcgctg
     4621 gcgatcatgg acgttaacca aaacgcggta gtcagtgcga tggaaaaaca tcaggtgcaa
     4681 tggctgatcc acggacatac ccatcgcccg gcggtacatg aacttatcgc caaccagcaa
     4741 cctgcttttc gcgtggtact cggtgcctgg catacggaag gttcgatggt gaaagtcacg
     4801 gcggatgacg ttgagctgat tcattttccg ttttaaaaaa cccacaactt tgctgatttc
     4861 acagccacgc aaccgttttc cttgctctct ttccgtgcta ttctctgtgc cctctaaagc
     4921 cgagagttgt gcaccacagg agttttaaga cgcatgtctt cccgcaataa tccggcgcgt
     4981 gtcgccatcg tgatggggtc caaaagcgac tgggctacca tgcagttcgc cgccgaaatc
     5041 ttcgaaatcc tgaatgtccc gcaccacgtt gaagtggttt ctgctcaccg cacccccgat
     5101 aaactgttca gcttcgccga aagcgccgaa gagaacggtt atcaggtgat tattgcgggc
     5161 gcaggtggcg cagcgcacct gccaggcatg attgccgcca aaacgctggt gccggtgctg
     5221 ggcgtgccag tacagagcgc cgcactgagc ggtgtcgata gcctctactc catcgtacaa
     5281 atgccgcgcg gcattccggt gggtacgctg gcgattggta aagctggcgc ggcaaacgcg
     5341 gcattactgg cagcacaaat tctcgcgact cacgataaag agctacacca gcgtctgaat
     5401 gactggcgca aagcccagac cgacgaagtg ctggaaaacc cggacccgcg aggtgcggca
     5461 tgaaacaggt ttgcgtcctc ggtaacgggc agttaggccg tatgctgcgt caggcaggcg
     5521 aaccgttagg cattgctgtc tggccggtcg ggctggacgc tgaaccggcg gcggtgcctt
     5581 ttcaacaaag cgtgattacc gctgagatcg aacgctggcc ggaaaccgca ttaacccgcg
     5641 agctggcgcg tcatccggcc tttgtgaacc gcgatgtgtt cccgattatt gccgaccgtc
     5701 tgactcagaa gcagcttttc gataagctcc acctgccgac cgcaccgtgg cagttacttg
     5761 ccgatcgcag cgagtggcct gcggtgtttg agcgtttagg tgaactggcg attgttaagc
     5821 gtcgcactgg tggctatgac ggtcgcggtc aatggcgttt acgtgccaat gaaaccgaac
     5881 agttaccggc agagtgttac ggcgaatgta ttgtcgagca gggcattaac ttctctggtg
     5941 aagtgtcgct ggttggcgca cgcggatttg atggcagcac cgtgttttat ccgctgacgc
     6001 ataacctgca tcaggacggt attttgcgca ccagcgtcgc ttttccgcag gccaatgcgc
     6061 agcagcaagc gcaagccgaa gagatgctgt cggcgattat gcaggagctg ggctatgtgg
     6121 gcgtgatggc gatggagtgt tttgtcaccc cgcaaggtct gctgatcaac gaactggcac
     6181 cgcgtgtgca taacagcggt cactggacac aaaacggtgc cagcatcagc cagtttgagc
     6241 tgcatctgcg ggcgattacc gatctgccgt taccgcaacc ggtagtgaat agtccgtcgg
     6301 tgatgatcaa cctgattggt agcgatgtga attatgactg gctgaaactg ccgctggtgc
     6361 atctgcactg gtacgacaaa gaagtccgtc cggggcgtaa agtggggcat ctgaatttga
     6421 ccgacagcga cacatcgcgt ctgactgcga cgctggaagc cttaatcccg ctgctgccgc
     6481 cggaatatgc cagcggcgtg atttgggcgc agagtaagtt cggttaactg gtgctctatt
     6541 cttgccggat gcggcgtaaa cgccttatcc ggcctaccga tccggtaccc attgtaggcc
     6601 tgataagatg cgtcaagcat cgcatcaggc attgtgcacc aattgccgga tgcggcaccg
     6661 gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc acaaatgtct aatgcctacg
     6721 actacagcga aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg
     6781 ccccaatcca cgcgggttta ccacggcttc tgacataacc acttaccgcc gttaccttcg
     6841 gccccatcga accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa
     6901 tggcgcgttg ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca
     6961 ccagcccatc ggcgttaatc tgctcggcaa gcaaggcagc ggcgagatct ttatcaatca
     7021 ccgcttcact ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca
     7081 ccacatgccc ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt
     7141 gcggagacgc caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag
     7201 ccgcttccag tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct
     7261 gcaaaaacgc cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg
     7321 gcatctgcgg ctgtgcgctc aagctctgcg ccagcatata gccaatcatc ccctggcttt
     7381 ccgccaccag cacatccagc ggatacggtt ctacctcttt ccacgccaga ttctgtaacg
     7441 ccagcagccc cacctgcggc ccgttgccgt gaacaatcgc cagccgataa gaacgggcca
     7501 ggcgtgccag cgcgggtaca gcactggcga tattgcgata ttgattttcc gccgtcagcg
     7561 cctcaccgcg ctggagtaag gcgttgcccc caagagctac aaccagtgtt ttcatggttt
     7621 tccttgtaat aattgttggc caagccagaa ccccagcagc gtatctgccc ctgacgtatg
     7681 ccccagcgcc agcagcgaat caatcgcaac agcggtacgc ttcgggcaac tcagagcatg
     7741 aacaaagtgc aggagtggcg aggcgaaata tccttgcgcg gcataacgta aataactgac
     7801 gctcaccgct gtggtaacga gttgaagatt gtcggaacag gcaaaaaacg ggcgaccggc
     7861 gcgcgcatct aaagcgccat aataccaggc cgccagcagc attccgctca acgtgtcatc
     7921 atgacttggc gttaatccgg ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc
     7981 attgagcgcg gcctgaaaac agtgacgaaa ctggcgtaat tcagcgggca gcggatcgct
     8041 tgccgccaac gccagtggtc cgaaaagtcc ggtttcctcc gcgcgttgca tccatgcagc
     8101 tgcaagcggt tgaggatgcg aaggcggcgt aatacgcagc aaacaataac gctgtggctg
     8161 tttaaccgtg aaacgcccga ggcgaatccc ttgagccaca acctgtggtc gttcattgcc
     8221 gcataatcca ccgcataacg catcgaattg cgcgcggcga agcacccatc ctccggggcc
     8281 gaaaccacta ccctgacgat gcagcgttaa cagttcgccg ctttccgtca tcaagttaat
     8341 cgcccgtcgc cacactcccg ctaaccgcca ggactggcga taattcggtg cactacagct
     8401 ggcaagcaga ggatggatga tcgtcattgc gcgttcctca accaatcccc atgctttcag
     8461 ccagtgcttc cagcgcctgt tcaaaacacg ccagcggtgc ccgcacagtg cctgcgccaa
     8521 tctgcccgat ccccgcctct ttatgggcga taccggtatt gatgagtggc gtaatgccgg
     8581 tttctaccac gcgacgaatg tccagtccca ggcacgcgcc ctgaaaatcc cagcctggga
     8641 tctgcaactg catattgcgc tcaaggtaaa tttccgccat ctcttcagat accgctctgg
     8701 ccgcttccat gccacccgca ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg
     8761 cacctccgat accaaaggtt tcggtaatcg cgctatcgcc catatccggg ttcgcctgct
     8821 cctgcgagaa gccggtgaaa aacagacctt gcggagtgtt tacaggcgca gtaaaccagc
     8881 gttcgcccag cccgcttacc cgaatcccga acatattgcc gttgcgggtc attgccgtga
     8941 cgatgctgcc tgcgcggatc atcgcgccag catccatcgc cgccttgcag taagccatcg
     9001 cgaggttgag gaagaactga tcggtcacgc tgaggaaatc catcacttcg gcgatgtgct
     9061 gtttatcatg atcgaggcga gcaatttgtg gggccagcgc acgcattaac agtgcagagg
     9121 aagcaatatt gcgttgatgg aactcatcgc ccatcgtaat gccctgcgcc atcatcgccg
     9181 tgagatcgat accgcgctcc atgcgcccca gcgccgcgct taatactggc attaacacat
     9241 cgcgcatcca gcgatggcga gtcaggacat cttcgccgta agcgccaaaa cgcatcactt
     9301 tgccgatacc ttcgttgagg ttgcagtacg cccggttgcc gtcggtcacg ttctcgacca
     9361 ccagcatcgg catactggca gaagtaatac cgcccattgg accgacggca ttcacatggt
     9421 gacaaggaat gaagttcact tccccctgct ccagtattgc cagcgcctgc gcttcatctt
     9481 tcgcccagcc ttcgaacaga catgcgccca cgcacgcccc tttcatgggt ccggtcatct
     9541 cctgccagcg cattggcggc ccggcgtgaa gcagtgtttt gccctcgttt agttcgctga
     9601 taagtgaaga agccggttgc acatccagcc agtgtggacg agcgcgacga atttgttcga
     9661 taaccgcagc attggcttgc gccactgatg taaacatggg aaccccttat tgcaaacgtt
     9721 ctaataaacg agccagtttt ttattgccgc cggcgactgg cgaccattgg taatgcacaa
     9781 ccggtttgct ggcgctttgt agctccagcg caaagctgcg taatccgatg ttaatcacgg
     9841 cgacgttttc cagtaatgac ggtgtgtgtt gctgagtggc aggcgaaagc ggacgaatca
     9901 gtgccgccgc cagcaaggtg gcttccggca gcgaactcac taccgcgatc cctgcatctt
     9961 ccagcgtggc gatttgctgc gagcggcatt gcgggtcacg ttcagtgcct gtcaccgtgg
    10021 caatggcata cagtggttga ttgtctgaac gcgcggcaca ggctttttgc caggcgctta
    10081 ccagcgaggc ggcaggatcg gcggtcgcac cgaagccaat cacgacatca agcagcaaca
    10141 cgcgcacttg cggtttagcg ccgagatcgg caattaactg attgcgtaag gctgggtcga
    10201 tcatcggatg gggacgcccg acggtgtaga aatcatcgcc gaggtcaata atctggtgac
    10261 catcggcgtc gagcatcatg ccgtgatgat gggtatcgtc ggcttccacg ccaaggtgcc
    10321 cggcaagtaa tcccgccgct tcggcagcca gcgtaccgcc ggtatacaaa ccgcaaataa
    10381 atccgctgct ggcaggcgtt atcgcgttac gtcgcgccgt aacgcgtgaa agcaggcaag
    10441 ccaggcgtgc ggcctcatcc agcgaggagg caaaccagac attctcgtcg caggccaccg
    10501 ccggggtata acctaaaaac agcgccaccg tcggtttgcc ggttgctttc atggcattaa
    10561 caattttcag acgcacagct tcggcaggcg gttttgaaac aaatgccagc acttcgcttt
    10621 tctcgtctgc actgagcatt tccagcgccg tcagcgcact gatgccgccc acttcacggc
    10681 tgaggtcgcg cccgccaagg ccaatcgcgt gagtaattcc ctcccctgcc agcgcaatct
    10741 gtgagcacag ctcctgaatc ccggtaccgg aagcgccaat gacgccaatg ttgccttccg
    10801 gcatcacgtt agcaaaagcc agcggcgtgc cggcaatcat cgacgtaccg cagtccggcc
    10861 ccatcaccag caagcctttt tctcgcgcgc gggttttaag ttggatttca tcttccagcg
    10921 tgacgttatc ggagaacatc atcacgttga ggttgcgatc cagcgcctga ttcgccagct
    10981 ccgccgcata ctcgccagcc actgaaatca gcgccagact ggcatcggat aatttctgac
    11041 aggcactgtc ccagcgacgc acctgcgtca aggactggct gctgcctgac ccctgcgcca
    11101 gttgttttag cgcctcttca agctgctgca taatcgcctg cgcgatcccc gcatccgccg
    11161 cttcgctacg aattgccacg caaatatcgt tcggcgtggc gttattaaaa tcgtcatgcc
    11221 agaaacctgt ggtatctaat aacgctttat tggcgggcgt acccatcatt acggaaacat
    11281 catcaacatt ttctgattcg ctgagttttc gtgaaataat cattaaactg accgaatcct
    11341 gaaaacaccc ttttttaata aaggcgtgga tcataacaac tccttaaaaa ttgcacccat
    11401 ggcaattgca cgagcagtgt taatgtggcg attacggtat cgatgacaac aagtaaataa
    11461 ggtgatgaat atcacacagg aattattaac aaaagaattc aaataaccgc caggctatta
    11521 cgcattattt tttaataagc gtggttgttt taaaaatcag gttaaaagtg agcggattat
    11581 caaataaatt ctaactatat ttttttgcct gtctggatca cataatccag atattttccc
    11641 tgtatgttaa ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca
    11701 gtcgggaaac actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg
    11761 agcacgctgc aaccgtggct gaagtattgg tttacgccga tgccagaggg atccactctc
    11821 atggcgcggt gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg
    11881 aaccggagtt tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg
    11941 ccgccggaca ggtcgcggcg aaaatgggta tggaacatgc catcaaaacc gcccagcaaa
    12001 atggcgttgc ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg
    12061 tgcagcaggc agcccgcgcc ggattaattg gtatttcgat gtgccagtcc gatccaatgg
    12121 tggtgccgtt tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc
    12181 cgggagaagg cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa
    12241 aagtgctcga cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa
    12301 acggtgcacc aacaaccgat ccgttcgcgg tacatgctct gctccccgcc gccgggccaa
    12361 aagggtatgg cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt
    12421 tcgggcgaca ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat
    12481 tacatatcgt tattaacccg aactttttct tctccagcaa attatttcgt caacatctta
    12541 gccagaccat gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt
    12601 atcccggaca ggatcaggat attaaacaac gcaaagccgc cgtcgaaggc atcgaaattg
    12661 ttgatgatat ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga
    12721 aaaatccctt tgcgcaataa ttattgagac aggactttct tatgattaca cattttcgtc
    12781 aagccataga agaaacgctg ccctggcttt cctcttttgg cgctgaccca acgggtggta
    12841 tgacccgttt actttattcg ccggaatggc tggaaactca gcagcaattt aaaaaaagaa
    12901 tggcagcaag cgggctggaa acacgtttcg atgaagtggg gaatttatat ggtcgcctga
    12961 gtggcaccga atatccacaa gaagtggttc tgagcggttc gcatatcgat accgtggtta
    13021 acggcggtaa cctcgacggg caattcggcg cgctggcggc gtggctggca attgactggc
    13081 tgaaaacgca atacggcgcg ccgctacgta cggtcgaagt ggtggcgatg gcagaagaag
    13141 aaggcagccg cttcccgtat gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc
    13201 ctgacgacgt gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg
    13261 cttgcggatt tactcttccg aacgccccac taactccgcg tcaggatatt aaagcctttg
    13321 ttgaactgca tattgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg
    13381 tgaatgcaat tgtcgggcag cgtcgttata cggtaacgct gaacggcgaa tcaaaccatg
    13441 caggcaccac gccgatgggt tatcgtcgtg atacagttta cgctttcagt cgcatttgcc
    13501 atcagtcggt cgaaaaagcg aaaaggatgg gcgatccgct ggttctgacc tttggcaaag
    13561 tagagccgcg cccgaatacg gtaaatgtgg tgccgggtaa aaccacgttc accattgatt
    13621 gtcgtcatac cgacgctgcc gtgctgcgcg atttcaccca acagttagaa aacgacatgc
    13681 gggcgatttg cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac
    13741 ccgtgccgat gaataaggag ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac
    13801 tgaattaccg ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg
    13861 taccaacctg catgattttc attcccagca tcaacgggat cagccataac ccggcggaac
    13921 gcaccaatat taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac
    13981 ttgcctggca gaaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc
    14041 gatgatttac tggctaaccg tgcgattgtt aaacacggta atttcgcact gttaaccccg
    14101 gatggtctgg taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc
    14161 acgccaaagc tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggtggc
    14221 aaccaacagg gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat
    14281 atcactgcca aagccgaagg caaaacattt gccttaagcg aaggtggcta tctttattgc
    14341 ccaccaggct ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta
    14401 tataagcgcc gctatgttcc ggtagaaggc catgcaccgt ggctggtttc tggcaatgcc
    14461 agcgaactgg aacgcattca ctatgaaggt atggatgatg tgatcctgct ggattttctg
    14521 cccaaagagt taggttttga tatgaacatg catatcctct cttttgcacc aggtgccagc
    14581 cacggttata tcgaaacaca tgttcaggaa cacggtgcct atattctttc cggtcagggg
    14641 gtttataacc tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc
    14701 gcttattctt tacaggctgg ttatggtgta gggcgtggtg aagcgttcag ctatatttac
    14761 tcgaaagatt gcaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta
    14821 gctatgttgg attttgccac ggtttatacc gtggcttttt ttgtagtaaa ttcaggtaaa
    14881 aaaagatgcg atataaatcg catccgcttt aaaggtaagg gtcagttttt aattccctga
    14941 cctattttaa tggcgcaggc aatatttcgt gcgctgttga agagattggt ttcaccgctg
    15001 gcgagcactt cggctaaagg tgccagacgt ggcaaaatgc tgaataccgc atcaatgccg
    15061 tactggtgca ccacttccac gccatcaccc aatacgccag caatcccaat caccggtaca
    15121 ttaaactgct tcgccaccga cgccacaccc agcggcgctt taccgcctgc cgtttgcgag
    15181 tcgatgcgcc cttccccggt aatcaccagt gctgcgccct gcactgcctg cgcaagattg
    15241 accgcattca acacaatctc aatacctggt ttaatatccg cattgagaaa taccgccgcc
    15301 gcaataccca taccgcccgc agcgccgccg cccgccatct ggcagacgtt gatttcagtt
    15361 tgctgttgta aaacacgggc gtaattttgc agcccctgtt ccagctcttc gaccatttcc
    15421 ggcgttgccc ctttttgcgg gccaaacacc gccgccgcgc cgcgtgcccc taccagcggg
    15481 ttatcaacgt cacatgctac ttcaatatgg caattcgcca gacgcggatc gcattcatcc
    15541 atctcaatgc ttgccacgcg cgctaaatta ccgccgttag ccaccagcac ctgaccgtcg
    15601 gcatcaagga aacgcacacc gagcgcctgc gccatgccca taccaccgtc gaccgtcgca
    15661 ctgccaccaa tgccgagaat aatatggcga atgccgttat ccagcgcatg acgaattaac
    15721 tcccccgtac caaaactgga ggccagcaac ggattacgct tttcaggcgc gaccagcatc
    15781 aggccacttg ctgccgccat ctcaatcacc gccgttttcc cgtcgccggt aaggccataa
    15841 aaagcattca ctttttcgcc catcggcccg cagacttcaa gcgtcacgat gttgccgccc
    15901 gtcgcggcga ccatcgcatc caccgtccct tcgccgccat ccgctatcgg caaacagata
    15961 tagttcgcat cgggaaagag ggtcgaaaac ccggctttaa ttgcctgaca acatttttct
    16021 gcacttaagc tctctttaaa agagtctggc gcaatgacaa tcttcattat caatcctttt
    16081 tcaacaaatt attcgatgcg ttcccacgga ataagattat ccataagaac agccagtaaa
    16141 atcccgacca gtaagccgtt actgagcaga ggacgcagcg ttaatggaag gtcttgcaga
    16201 tacacaggcg gtaatgccat taaaaatatg ccgacaaata acggcaatgc gagtcgataa
    16261 atattgcgag cggtaaacgt tatttgctgg ctaaacacca gcgcggaaaa gagtaaaggc
    16321 agataagaaa ccagcatgac cgcactactc acgggtaaag ggatactgca aaacagtcgt
    16381 gtgagtgccg gaaccagcgc caccagaagg caaataacgc tgccataaat aaatgaacgc
    16441 cgcgtgtaat cgccagtctg ggttaataaa ccaatggatg aaacaaacgg tgaaaatgga
    16501 attaccgcca gaggtacggt tatcagtgtc ataaatccgg tcgccacaaa gctacgacga
    16561 taacgcgtat tccccgcgcc ctgctgcgga taaaaaacat ccgtgccccg aatcgcaccg
    16621 taggtattgc tgatatttac cagacctgta atcaccgccg tcagaataat tcccggcgac
    16681 aaagcaccgc cactgccgag cgggaaccac tgccagtgca actcaccgga gagcgagtgc
    16741 gaagaaggaa agcaaaagta ccacaacaac cagccggtta tggtgccgac cagcaggcca
    16801 taacgggcaa aacgttgcgg gaggaagata atcatcccca gtaccaggca catcaccgcc
    16861 accgagagcg cgaacggcgg taactgaatt ttaaaattcg ggtcggctat gccaaacggc
    16921 agcccgagca tacctttgaa aaagatagtg gtcagctgcg cgcccagcat caacataaac
    16981 aagaccatca ccgacggcgt aaataaccgc gccaggcgat ggcctaatcc gctaaaacca
    17041 atcaacaccg tcagcacgcc ggagagtgca atccccactg ccaggctggt ggcgatatcg
    17101 ttgatcggtg tcccgcgcga tgcttcgcca agggtgatag taaggatggt tccccaccac
    17161 aggccacctg gcccttccat aatagcgcga cgatgaccgc agaacgcctg agcgaagcag
    17221 gccagtgcgg tagcaagaaa agcatattgc gtgagcgtaa gcaggctact ttgcggcaac
    17281 tgaaaagcag aaagtagcgt aggaggaacc acaaccgtgt tgcaaaaaat aaagaaaaac
    17341 cactgaaatc ctgataacag gctttcgcgg ctcactgcaa aattgaacat aaacacacct
    17401 taaccggaga atgcccgccg caaggacggg cattgcaggg gccagattac tgctgatgtt
    17461 taaggataaa ttgacctttc ggcgcaacag ggaagccctg ttcaatgtcg taaatcacat
    17521 caccatgtaa gatggttttc gtgatacgcg cgccaatggt acggccaaca tacgggctga
    17581 ctttgtggcg atattccaga tcgtcattgg taagaacata gctgctattc ggctgaatga
    17641 agacgaagtc ggcatctttt cctggggcga tacggccttt ttgctgcaga ccgaaaatat
    17701 ctgctgcgtt agtcgccatt aatttgccga acattggcag agacattccg cgtttctgta
    17761 ccgcttcatc gaacatcacg tccatacagt tttgcaggcc ggcaataccg ccccatgctt
    17821 ccatgatgtt gccggctttc atttccggag ggcatggtga gtggtcggaa accaggcagt
    17881 cgatttcacc gttaaacagt ttttcccaca tgcctttctg gttttccaga tcgcggatcg
    17941 gcggtgaaca cttcgccaga gtgccgattt cttcgaactg atcggtatcc agtacaaagt
    18001 aatgcgggca ggattcacaa gtaacgtcct gaccttcctg acgtgcacga gtcacttctt
    18061 caacgccttc cgggctgctg atatggcaaa cgtgcagacg gcaaccggca actttcgcca
    18121 gatacagcac gcggcgaatc gcttccactt cggtaaatac cggacgcgaa gccacatagt
    18181 catgggcagt tacgcgacct tcacgtttcg cttcttcgcc cagtgcgtca cagatcagcg
    18241 cgttttcgca gtgcaccagc accggctgcc ccagttcgcc cagcttctgc gcacctttga
    18301 agaactgcca gtcgtttaca tcacggaagt cgttgtcgat accgcgatcg ccacaggtcg
    18361 caacgaagca tttgaagccg acaacgccca cttcatccag ctcatgcaga cggtcgatgt
    18421 tgtaagacac caggccaccg agttgtgccg catcgatagt cagcttgcct ttagcggcat
    18481 cgaacttcag ttcaattgaa gcgcggtcaa ccgttgcagg cagctggttg agcggcattt
    18541 cgatcatggt ggtgatacca ccttttgccg ctgcgcgagt accggtttca taaccttccc
    18601 agtggctacg acccggttca gaaatatggg tgtgcgcatc aaccatgccc ggcgaaacca
    18661 ccagaccaga cgcatccata acttcttttg catcgcccag atcctgaccg atagcagcaa
    18721 ttttcccgcc tttaacggcg atatctacaa cgcgagcttc gttttctaaa ataacggtgc
    18781 cgtttttaat gattaaatca aaagacataa caaactcctt gtaaaataca gattggtttt
    18841 taactcaatg ttcaatatcg ggattaatta acctatggtt ttttgctctc ctgttttttc
    18901 tactgttgta cgtttcttta ataaggcgta ggccgcaaag gcgacgatga cgccgacaaa
    18961 ccatgaaaca cgcgataacg gttccataaa gggaataaac ttaccgccaa gagataaaat
    19021 aacggccacc agagttactg aaaacgcggt gaggttaaaa ccgttatcgt aatatttata
    19081 atcgccaggt gcggtgtaca gttcatcaag attaatttgc ccacgcatca ccacaaaata
    19141 atgtgccatc atgacaccaa ttaccggacc cagcattccg ccgatgatat cgaggaacag
    19201 ataaatactg tcctgatttt ccattaattt ccacgggcag atcagcaggc tgataatact
    19261 ggcaatcagt acgccgtttt tataggtcag ttttgtcggt gcaatggcgg caatctgata
    19321 accggccgga ataatattac cggtggcgtt agtggaaata gtcgtcatca gaataaccag
    19381 taccgcaaag aacgaggcaa acaggctgtc ccaacgctga acaatatcca gcacgttcca
    19441 ggtgtcagcg ccgtagtgaa tactggctcc ggcaataata cataccccag cgaccgcaaa
    19501 cagaatatag gccacaacta aacccagcgt ttgccccagc gcttgctcac gaaacgagtg
    19561 ggcgttttgc gtaaagtcgg atgcgctcac cgccggtgcc gcccagaccg caactaccgc
    19621 gttaatcacc accaggaaca agaatccact gttttctgct ttctgaatac cgctcggaat
    19681 gtagtcaaag attggaccga tcccgaccag tgaaatcgcc caaatcgcca tgccgccgaa
    19741 aacgatatag atgcacgggt taagaatggc agtgaattta tttaaaactt tgccaccgcc
    19801 aaaaccaata ccaacgttaa ccagccagaa gagtaagaaa gtaattaagc ccggtagcga
    19861 aaggcctaac aaggtaaaat caccaccgag agttaaaaat cccggccaga ttttgccaat
    19921 caggatcaag caggccagtg atccggcgta acattgcagg ccaaaccaca taatggcagc
    19981 aataccacct cttaataatc cgggaaacag tgcgccacgt acgccgtaag aagcacgcag
    20041 gatcatggca aacggtacgc cgtatttact gcccgcagca ccgtttaata ccattaccgc
    20101 ggcaatgaaa aaggcgctga ggataattgc cagcataata ctaaaggtag acaagccgag
    20161 aataaaaaag ccgccgacca tcacataatt gggaacgtta tgaaccgaac ccatccataa
    20221 ggtaaaatag ttaaatgttt tccaggtccg ctggctttgc gttttcggca ataaatcttc
    20281 gctatagccg cgttgctgga atagctttct ctgatgttcc ataaatccct cttaatttca
    20341 cgtatcgaat aacgttgttt taatctagct atgaggaaca aataaaataa aaaatattat
    20401 ttttaatact gaagcaacat ctttggctgc tttctttttt aatattgctg gccgattcat
    20461 tttctgatat cggccatttt tattgcgggt atcaggccag tttatggtta gccattaatt
    20521 ccagcgcctg cactaacgca gagtgatcca actggctgcc accgtttgcc gcacaggtat
    20581 taaataactc ctggcaggtc gcagtgtttg gcaggttcag cgcaagtgct ttcgcacttt
    20641 gcagtgccag gttgagatct ttctggtgca gagcgatttt gaagcccgga ttaaaggtgc
    20701 gtttaatcat acgctcgcca tgaacttcca gaatacgtga ggaagcaaag ccgcccatca
    20761 gcgcctggcg cacacgtacc gggtccgcac cggcttttga agcaaatagc agggcttcag
    20821 aaaccgcttc aatattgagc gccacgataa tctgatttgc cactttgcag gtttgaccat
    20881 cgccgttacc gcccacgagg gtgatatttt taccgagcag ttcaaacagc ggtttaacac
    20941 gttcaaatac cgcttcatca ccgccaacca taatcgacaa cgtcccttca cgcgcaccga
    21001 tttcaccgcc ggagactggc gcatcgagat aatcgccgcc cagttcattc acctgacgag
    21061 cgaaacgctt agtttcaatc ggggaaatgg agctcatatc aacaatggtt ttgcccttca
    21121 gcgaagtttt tgcacagcca ttttcaccaa acaatacttc ttcaacctgc ggcgtatctg
    21181 gcaccataat aaagataatg tcagaagatt cagttacctg gcgcgcggtt tcaacattta
    21241 ctgcgcccag cgccagtaat tcttccgcaa ccggaccaat ggtcgtcaca tgcagttgat
    21301 ggcctgcgcg tgccaggtta actgccatcg gtgtgcccat aatacccagg ccaataaatc
    21361 ccagtttcat aattaacctc ttgtgaatat cgctgtttct gaattcagac aacacgatcc
    21421 cctgccggca tagcggcaaa aaatgcattg tctgaatcgt ttttcgtttt agcggtatgg
    21481 attaatccat gataatcccg cttccgtggt ggtcaggggt ttatattcgc accctaccca
    21541 accgtcataa tcagactttt caattaaatc gaataaaaag tcgtaattta tttccccggt
    21601 gccaggttcg ccacggcggg ggttatcggc aatctgcaaa tgaccgattt tatttgccca
    21661 tgtagtcatg gtttgggcta attccccttc catgcgttgc atgtggtaaa tatcgtactg
    21721 gatcttgata ttgttactgc caatatcggc aatcagcgcc agtgcttgtt gtgttccggt
    21781 caaatggaag cccggcatat caaagtgatt aagtggctca atcagcagca aaataccctc
    21841 ttttgccagc atatttgccg catagcgcag gttctctacc agagtggtat gtacctgctc
    21901 tgctgatacg cccacaggtg ttttaccaac cagacagtta atttttttat tacccagtgc
    21961 acgggcataa cgaatcgcaa cggctacacc atcacgaaat tcctcttccc ggccaggaat
    22021 acaggctatt ccgcgttcac ccgccgccca gtcacccgca ggtaaattat gcagcgtatg
    22081 ctccaggtta ttactgctga gcttgtgctg caaaacttct atgtcgtagt cataggggaa
    22141 cataaattct acagcgcgaa atccgcactg cgccgcttta tcaaagcgct caagaaaatc
    22201 atattcctgg aataacatcg acaagttagc agagaaacgt aacattaatt acctccctta
    22261 cttatattgc ataaaacagg tttcggttgg cgcgtccgct gcgttatcgg cgatatcttc
    22321 aaattccatc acattatcga gttcgttacc catcgaaata ttggttacgc gctcgagaat
    22381 gatttccacc accacaggta cacggtattg attcattaat gctttcgcct gttcaaatgc
    22441 cggagcaata tcttccggtt tgaaaacacg aatcgcttta caacctaaac cttccgctac
    22501 ttttacgtgg tcaacaccgt agccattcac ttcactagag ttgatattct cgaaagcgag
    22561 ttgcacgcag tagtccatgt caaaggcacg ttgtgactga cgaatcaggc cgagataggc
    22621 gttattcacc agcacatgga tgtacgggat gttgaactgt gcgccaacgg ctaactcttc
    22681 aatcaggaac tggaagtcaa agtcgccaga aatcgccacc acattgcgtt tcggatcagc
    22741 ggcacaaacc cccagcgccg ccggaatcgt ccagcccagc gggcctgcct gcccacagtt
    22801 gatccagtgg cggtctttaa agacgtgcag catttgcgca gcggcaattt gcgacagacc
    22861 aatggtggtg acatagcaaa catcacgtcc aaaggctttg ttcatctctt catacacgcg
    22921 ctgcggtttc accggaacgt tgtcgaagtg tgttttacgc aacaaagtac gtttgcgctg
    22981 ctggcactca gcaacccact ctttacggca tggcagacgc cctgcttttt gcatttcctg
    23041 cgccacttca accagtaaag tcagggcagc tttagcatca gagacaatcc ccaaatccgg
    23101 gcacagcacg cgaccaattt gcgtcggctc aatatcgata tgaacgattt tgcgcccttc
    23161 ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa cggttaccga taccaaacac
    23221 catgtcggac gccagcaacg ttgcgttacc gtaacgatgc gcggtttgca gacccaccat
    23281 cccggccatc agttcatgat cgtccgggat acagccccag cccatcagcg ttgggatcac
    23341 cggaacgctg gtcagttcag caaactgttg taacagtgca gctgcgtcag cattaattac
    23401 cccgcccccg gcaacaatca ccggacgttc ggcctggatt aacatttcta cagctttttc
    23461 gatctgcata cggctggcag caggtttgta gaccggcagc ggttcgtaca tatcaggatc
    23521 aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc accagtaccg gacccggacg
    23581 accagaacgc atcaggtgaa atgcctgttg caacacacga ggcaccagcg ccgcttcacg
    23641 tactgtaacc gccattttac ttaccggctt ggcgatagct tcgatatcaa cagcctggaa
    23701 atcttcttta tgcagacggg cgcgcggagc ctggccagta atacacagaa taggaatgga
    23761 atcagcagaa gcagaataga gcgcggtgat catgtccgtg cccgcaggac cggaagtccc
    23821 cagacatacg ccgatattcc ctgccgttgc gcgggtataa ccttccgcca tgtgcgaagc
    23881 accttccaca tgacgcgcca gaatgtgacg aataccgccg tgcttacgca tcgctgagta
    23941 gaacggattg attgcagctc ccggaacgcc gaaggcggtg gtgatacctt ctttctccag
    24001 cacatacatt gccgcgtcaa cggctctcat ttttgccatt ttattcctac ctctattaat
    24061 tggaaaaatt ttccaacttt caaattttgt caaaaaaacc cgctccgcag agcgggatgc
    24121 gacagacact atggatgtgc tttcagtccc aacgccgtgc tgatatcgcg ggcggtgtct
    24181 ctgaccagct caccctgact gacaaaacga tcttctgtca gtcttgatga aggcccggag
    24241 atggagatag cggcaacaac gctaccgaca tcatcgtaaa ttgctgaagc tatgcaattc
    24301 agacctacaa catgctcttc tttatctacg gtatagccca gttcacgcgc ttgttccagg
    24361 tccttcagca aggtgggcat atccacaagc gtagttggcg taaactgctg caaaccggtt
    24421 tgcagaatga tgctcatcaa ctcctcttcc gccagcggat aaagcagcgc tttgcccgca
    24481 ccggaagcat gcagcggcag acgactgccc agtggcgcac acatcctgac cattgattta
    24541 cactctaact gaccaattaa tactgcttca ttgccgttac ggatcgcgac attgaccgtt
    24601 tcgccggaaa gtaacatcag gcggcgcata aacggcccgg cgacggagag gacatcgcgg
    24661 ttatggatgt acgccgcacc gacgttaaag acacctaatc ctatatgcca ccagcctaat
    24721 tgactgtcct gatagacaaa atccgctgcc tgtaaaacct tcagcaagcg aaaggtcgtg
    24781 gagagcggca aatccagatt gagagaaata tcgctaaccg acgaacttcc cccacttttt
    24841 tccagatatt gcagaatcgc aattccccgc tctaacgcct gtgcgccctt ctgtgccaca
    24901 ggctccgcct gtcctggcct gccgcgccgt ctaacttccg tcatacctac gttaactccg
    24961 acctacaagt gagcataaac aatgaacaat tagtttatct tatgcaagtc ggttgcaggc
    25021 gtcacgcaaa acagagttcc tgttcaggaa tactttcaac atcacagttg tcactgccgc
    25081 cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc gaaaagtggg tgatgccaga
    25141 cgttacgatg gtaattcact ccctgttcgc cgttggtgat aaacgcccgc agcgttgaca
    25201 ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa cacttcacct ttcatcggga
    25261 taaaggcctg agtgcccagc ggatgacgtt cgagttcgta aatggtcagc ggcagattcg
    25321 ccggttgcgc gcggttaatg ctgataagcg tacggtcttg ctcaagaatc tcaaccagcg
    25381 ccaaatcgtg gtaacgctcc accaggccat tgttaatatg gaaaaaatcc cgttgctgag
    25441 tttcgattac gtcgccataa gcactaaagg cttcctgact taacggtaat acctgaagtt
    25501 tcatcttttt gtccttgtaa cataattccg ttcaaccatc gccgaccggt cagcttggtt
    25561 tcaataacag gcaatctaaa accttaagag aacaacatca atacaaaagt tggaatattt
    25621 ttccaaaact agatttagtc cacaaattga acagggttat catgtaaaaa tactgataaa
    25681 tcagaggata agaaaatcac gcaacttgcg gatttaaaag gggatacctg aagatgttcg
    25741 atccagaaac cttgcggact ttcattgcgg ttgctgaaac aggaagtttt tcaaaagcgg
    25801 cagaacgatt atgtaaaacc acggcgacga tcagttatcg cattaaactt ctggaagaga
    25861 ataccggagt agcgctgttt ttccgcacga ctcgcagcgt gacgttgaca gcggctggcg
    25921 agcatctact ttcccaggcc agagactggc tgagctggct ggaaagtatg ccaagcgagc
    25981 tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat tgtcatcaac aacctgctct
    26041 acaaccccca ggccgtcgcc cagttgctgg cgtggctgaa tgaacgttac ccctttaccc
    26101 agtttcacat ctcccgacaa atctatatgg gcgtctggga ttcgctatta tacgaaggtt
    26161 tttcactggc tatcggcgtc acgggaaccg aggcgctggc aaataccttt agtcttgatc
    26221 ccttaggatc ggtgcaatgg cgctttgtca tggcggcgga tcatccgctg gcgaacgttg
    26281 aagagccgct aacagaagcg cagttgcggc gctttccggc ggtcaatatt gaagacagcg
    26341 cccgcacctt aaccaaacgc gtcgcctggc gattgcctgg gcaaaaagag attattgttc
    26401 ctgatatgga aacgaaaatc gccgcccatc ttgcgggcgt tggcattggt tttttgccaa
    26461 aatcgctttg ccagtcaatg atcgataatc aacaactagt tagccgggta atcccaacga
    26521 tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt tggcagcggc aaagcggtag
    26581 aagatattgt gaccttgttt acccagcgca ggccggaaat cagcggattt ttagaaattt
    26641 tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg ttggcgcgta aaatagcgcg
    26701 caagctgatt tgaggttcac ttttaactat gcaagagaga cacacggaac aggactatcg
    26761 tgccctgctg attgctgata cgcccattat tgatgttcgc gcccctatcg agtttgagca
    26821 aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat aacgatgaac gcgccgccgt
    26881 tggcacctgc tataaacagc aaggctcaga cgcagcgctg gcgctggggc ataaactggt
    26941 ggcgggtgaa attcgtcagc agcgcatgga cgcctggcgg gcagcgtgcc tgcaaaatcc
    27001 gcaaggtatt ctctgctgcg cccgtggcgg tcagcgctca catattgtgc aaagctggtt
    27061 gtatgcagcg gggattgatt atccgctggt ggaaggcggt tataaggcac tgcgccagac
    27121 cgcgattcag gcgactattg aactggcaca aaaaccgata gtgctgattg gcggttgtac
    27181 tggcagcggt aaaacgctgt tagtgcagca acagccgaac ggtgttgatc tggaagggct
    27241 ggcgcgccat cgcggttcgg cgtttggtcg cacgttacaa ccacaactta gccaggcgag
    27301 ttttgaaaac ctgctggctg ccgaaatgct gaaaaccgac gcccgtcagg atttgcgcct
    27361 gtgggtgctg gaagacgaaa gccggatgat cggttcgaat cacctgccgg aatgcctgcg
    27421 cgagcgaatg actcaggcgg cgattgcggt ggtagaagat ccgtttgaga tccgtcttga
    27481 gcgcctgaac gaagagtatt tcttgcgtat gcatcatgat tttacccacg cttatggcga
    27541 cgaacagggc tggcaggaat attgcgaata cctgcatcac ggactttcgg cgattaagcg
    27601 tcggctgggg ttacagcgct ataacgaact cgctgcccag ctggatacgg cgctgacaac
    27661 gcaactcacc accggcagca ccgacggtca tctggcctgg ctggtgccgt tacttaaaga
    27721 atattacgac ccaatgtatc gctatcagct tgagaaaaaa gcggaaaaag ttgtctttcg
    27781 cggtgagtgg gcggaagtgg cggaatgggt taaggcgcgg taatgaggga cgtatttgta
    27841 ttagtcgcga cttgatctga cacatggcct tgaaaggttg agagttaccg gttttgatat
    27901 gagtgtcgaa tccttatcca aaacaagagg taactctcat gcttcatact gccaatcccg
    27961 tcatcaaaca caaagccggt ttgctcaatc tggctgaaga actcagcaac gtatcaaaag
    28021 cctgtaaaat catgggcgtt tcgcgtgata cgttttatcg ttaccgcgaa ctggtcgctg
    28081 aaggcggtgt ggatgcgcag attaatcgta gtcgccgcgc tcctaacctt aagaaccgta
    28141 ccgatgaggc aactgaacag gctgttgttg attacgccgt cgctttcccg acacatggtc
    28201 agcaccgggc cagcaacgag ctgcgtaaac agggggtttt atctccgaca gtggtgtccg
    28261 ttctgtctgg ttactccata accttgagaa tctcaaaaga cgatactgaa caaattttat
    28321 caggtgacgt tccgtaaaaa gttgtatgga gttctcgatg cagtcggcgc gactatcagt
    28381 acccccgctg aaccttggac tatcgggccg ccgtactggg gcatgtcacc ctggcgtgcc
    28441 gctggttttc cgctaatggt ttatttcctc tttctttccc ggcaagaatt tattttaatc
    28501 cggaaggatt atttattttc agatagggac tcacattaaa cacgtaacat tttaattaat
    28561 gcatggctga atatgtatta cacaatgtca gatatcgtaa aagaccagtg caattctatg
    28621 taaactctct gactgttcat tttatttgtt gtttcagggt cggaactcac cctcaataaa
    28681 caactgaata cgagtagata ttcatcgtct gagctatatg gctttacaca atagccgaca
    28741 tgtcttttct cagtcaggaa ttttactttt cctcactacg aagttgacga cttcgatatg
    28801 ggatagactc ttaattcaag caattactct gcacaagcat caggcaatcc agctccaata
    28861 tttgttataa aataatgcgt aacattaatt tcaccaacaa aaaaaaggtt tacgctatcc
    28921 ccttgccctg gtattttacc atatttacac cacacttttt ttgtatctgg atcattctct
    28981 gatacgattt tcgccacttg ttccctggcc ctcaccgtca atgttttctt aaacctcccc
    29041 ccatcatcga ttgttaggga aaaataag
//
LOCUS       DAMZII010000054        29046 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZII010000054 DAMZII010000000
VERSION     DAMZII010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.054"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(92..1756)
                     /gene="glnS"
                     /locus_tag="POA77_002283"
     CDS             complement(92..1756)
                     /gene="glnS"
                     /locus_tag="POA77_002283"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDI6365719.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1959..3905)
                     /gene="nagE"
                     /locus_tag="POA77_002284"
     CDS             complement(1959..3905)
                     /gene="nagE"
                     /locus_tag="POA77_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDI6365720.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4238..5038
                     /gene="nagB"
                     /locus_tag="POA77_002285"
     CDS             4238..5038
                     /gene="nagB"
                     /locus_tag="POA77_002285"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6365721.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5098..6246
                     /gene="nagA"
                     /locus_tag="POA77_002286"
     CDS             5098..6246
                     /gene="nagA"
                     /locus_tag="POA77_002286"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6365722.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGQVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6255..7475
                     /gene="nagC"
                     /locus_tag="POA77_002287"
                     /gene_synonym="nagR"
     CDS             6255..7475
                     /gene="nagC"
                     /locus_tag="POA77_002287"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDI6365723.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7523..8275
                     /gene="nagD"
                     /locus_tag="POA77_002288"
     CDS             7523..8275
                     /gene="nagD"
                     /locus_tag="POA77_002288"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDI6365724.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8532..10196
                     /gene="asnB"
                     /locus_tag="POA77_002289"
     CDS             8532..10196
                     /gene="asnB"
                     /locus_tag="POA77_002289"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDI6365725.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10577..10653
                     /locus_tag="POA77_002290"
     tRNA            10577..10653
                     /locus_tag="POA77_002290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10611..10613,aa:Met,seq:cat)
     gene            10663..10747
                     /locus_tag="POA77_002291"
     tRNA            10663..10747
                     /locus_tag="POA77_002291"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10697..10699,aa:Leu,seq:tag)
     gene            10771..10845
                     /locus_tag="POA77_002292"
     tRNA            10771..10845
                     /locus_tag="POA77_002292"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10803..10805,aa:Gln,seq:ttg)
     gene            10880..10954
                     /locus_tag="POA77_002293"
     tRNA            10880..10954
                     /locus_tag="POA77_002293"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10912..10914,aa:Gln,seq:ttg)
     gene            10970..11046
                     /locus_tag="POA77_002294"
     tRNA            10970..11046
                     /locus_tag="POA77_002294"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11004..11006,aa:Met,seq:cat)
     gene            11095..11169
                     /locus_tag="POA77_002295"
     tRNA            11095..11169
                     /locus_tag="POA77_002295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11127..11129,aa:Gln,seq:ctg)
     gene            11207..11281
                     /locus_tag="POA77_002296"
     tRNA            11207..11281
                     /locus_tag="POA77_002296"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11239..11241,aa:Gln,seq:ctg)
     gene            complement(11435..12610)
                     /gene="ubiF"
                     /locus_tag="POA77_002297"
     CDS             complement(11435..12610)
                     /gene="ubiF"
                     /locus_tag="POA77_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDI6365726.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGESWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            12756..14180
                     /gene="miaB"
                     /locus_tag="POA77_002298"
     CDS             12756..14180
                     /gene="miaB"
                     /locus_tag="POA77_002298"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI6365727.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14333..15373
                     /locus_tag="POA77_002299"
     CDS             14333..15373
                     /locus_tag="POA77_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI6365728.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15370..15837
                     /gene="ybeY"
                     /locus_tag="POA77_002300"
     CDS             15370..15837
                     /gene="ybeY"
                     /locus_tag="POA77_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI6365729.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15927..16805
                     /gene="corC"
                     /locus_tag="POA77_002301"
     CDS             15927..16805
                     /gene="corC"
                     /locus_tag="POA77_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDI6365730.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16830..18368
                     /gene="lnt"
                     /locus_tag="POA77_002302"
     CDS             16830..18368
                     /gene="lnt"
                     /locus_tag="POA77_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDI6365731.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18446..20323)
                     /locus_tag="POA77_002303"
     CDS             complement(18446..20323)
                     /locus_tag="POA77_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI6365732.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSQLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20778..21686
                     /gene="gltI"
                     /locus_tag="POA77_002304"
     CDS             20778..21686
                     /gene="gltI"
                     /locus_tag="POA77_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDI6365733.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21856..22596
                     /gene="gltJ"
                     /locus_tag="POA77_002305"
     CDS             21856..22596
                     /gene="gltJ"
                     /locus_tag="POA77_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDI6365734.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22596..23270
                     /gene="gltK"
                     /locus_tag="POA77_002306"
     CDS             22596..23270
                     /gene="gltK"
                     /locus_tag="POA77_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDI6365735.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23270..23995
                     /gene="gltL"
                     /locus_tag="POA77_002307"
     CDS             23270..23995
                     /gene="gltL"
                     /locus_tag="POA77_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDI6365736.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24113..25048
                     /gene="rihA"
                     /locus_tag="POA77_002308"
     CDS             24113..25048
                     /gene="rihA"
                     /locus_tag="POA77_002308"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDI6365737.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25132..26802
                     /gene="hscC"
                     /locus_tag="POA77_002309"
     CDS             25132..26802
                     /gene="hscC"
                     /locus_tag="POA77_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDI6365738.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26862..28313)
                     /gene="djlC"
                     /locus_tag="POA77_002310"
     CDS             complement(26862..28313)
                     /gene="djlC"
                     /locus_tag="POA77_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDI6365739.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28310..29017)
                     /locus_tag="POA77_002311"
     CDS             complement(28310..29017)
                     /locus_tag="POA77_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI6365740.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7127 a         7551 c         7351 g         7017 t
ORIGIN      
        1 aaaaacgccg cagagtgcgg cgtttgggat tgtttgctat ttcttgccgg atgcgacgta
       61 aatgcctcac ccggcctggt agaacttaaa attactcgcc tactttcgcc caggtatcac
      121 gcagcccaac ggtgcggtta aataccggtt tttccgctgt ggaatggcgg ctgtcgaggc
      181 agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct ttcagcgacg
      241 gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat
      301 ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt tcaaccggca
      361 gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca tctgccggat
      421 ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg ccttcggcat
      481 ctttctccac gcgttctgcc ttaatgacgt aagcattacg cagacgcact tctttaccca
      541 gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa
      601 tctcaccgct aaacggcacc tggcggctac ccatttccgg tttgttcgga tggttcggca
      661 tggtaaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc ggatcgataa
      721 ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat tccagcgatg
      781 ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag
      841 aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc
      901 agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac atcacagtgt
      961 attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt
     1021 ccagcaccca gtcgtacagg cgacggttgt cctggaactc aagcgtacac agagagtgcg
     1081 taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc
     1141 acttgttgcc agtctggtgg tgttcagcaa atttaatacg gtacagcacc ggatcgcgca
     1201 tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa
     1261 aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt
     1321 acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg
     1381 tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct
     1441 gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcta
     1501 cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta gtgtcgtcga
     1561 aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt
     1621 tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg
     1681 tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta gtcgggcggg
     1741 cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc
     1801 ttataagcgg gacaggctga caactgttag ttttttaaaa ttgataatct aacaaggatt
     1861 atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt gtcattgttg
     1921 gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat ttcatacagc
     1981 ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact gaaatcgtcg
     2041 atattgctgc aaaccaccgg gctaatcatc gagcgggcgt tagcgttcag gtaatccaga
     2101 tccatttcca gaatcggttg ccctgcgctt acctgcgcac cctcttccac cagacgttta
     2161 aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat ctccgcgcct
     2221 ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg
     2281 gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc caccgctttg
     2341 ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt aatcggcgat
     2401 accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac aggcgcggca
     2461 gttgccggag ttgcttcagc tgacgcagct gctaccggac cgcgggcaac gactttcttc
     2521 atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc
     2581 agttttacta caccagaagc acccagacgt ttacacatcg tatcgttaac gcgggcagag
     2641 tcagccactg taaggcgcag acgggtgata caggcgtcaa ttgctttcag gttgtcagtg
     2701 ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc agtgttgctg
     2761 ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt tttcaggttg
     2821 aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac aacacccatc
     2881 accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg
     2941 atagcccccg cagagaaaga gaagccagca tggataccca gcagtgttgc cacaaacagg
     3001 ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa catgaacagg
     3061 aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga aagcagcatc
     3121 ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag cgccgcaccc
     3181 ggcagaccga acatcataat cgggaagaag ccggacatga acatccccgc ggtgccgtca
     3241 ccggcataga agcggttgat atcaccgtgg aaaaccgtac ccgccgcgtt ggtgaattca
     3301 ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg gatcagcaga
     3361 cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat ccactcgcct
     3421 cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc cgccagcacc
     3481 aggcagaaga agccggtggc aatcggcaca aagcgtttgc cgccgaagaa actcaggaag
     3541 tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag accggtaatg
     3601 atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc tttggttaac
     3661 acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg
     3721 gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg
     3781 gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag tgccgccacc
     3841 ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata
     3901 ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta tttttatttt
     3961 gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg ctacagagtg
     4021 tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa
     4081 atatcgttat catcactccc ttttactggc taaagcagaa aacttatttt atcattcaaa
     4141 aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat
     4201 ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc ccctgactac
     4261 cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa
     4321 accgactgcc gatcgtccgt ttgtactggg cctgccgact ggcggcacgc cgatgaccac
     4381 ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc acgttgtcac
     4441 cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct actacagctt
     4501 tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca accttctcaa
     4561 cggcaacgcc ccggatatcg acgccgaatg ccgccagtat gaagaaaaaa tccgttctta
     4621 cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg catttaacga
     4681 accggcgtct tctctggctt ctcgtactcg tatcaaaacc ttgactcatg acactcgcgt
     4741 cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg ccctgactgt
     4801 cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg gtagccagaa
     4861 agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga ccatcagctg
     4921 tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca tggagctgaa
     4981 agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt
     5041 gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg ggtcagaatg
     5101 tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga ccacgcggtt
     5161 gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc gccagagatc
     5221 gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt gcagttaaac
     5281 ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga aacgctggaa
     5341 atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac gcttatcacc
     5401 accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat
     5461 ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc
     5521 acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa
     5581 aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc
     5641 agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc gacgttgaaa
     5701 gaagcgaaag ccggtttccg cgcggggatt acctttgcca cccatctgta caacgcgatg
     5761 ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga agctgacatt
     5821 tattgcggta ttatcgctga tggcctgcat gttgattacg ccaacattcg taacgctaaa
     5881 cgccttaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc aggtgccaac
     5941 attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact ttgtgtggat
     6001 gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg taatctggtc
     6061 gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt
     6121 gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtcaagtagc caacctgacc
     6181 gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga ggtcgtaact
     6241 caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt gatctcgtaa
     6301 aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca atctcgcgga
     6361 ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc
     6421 ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg ggccgccgcg
     6481 ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc
     6541 acgacgccac catcaccctg tttgatctca gcagcaaagt gctggctgaa gaacattacc
     6601 cgctgccgga acgtacccag caaacgctgg aacatgccct gttgaatgcc attgctcagt
     6661 ttattgatag ctaccagcgc aaactgcgcg agctgatcgc gatttcggtg atcctgccag
     6721 ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag gtagaaaact
     6781 gggggctggt agaagctctg gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata
     6841 tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc gaagactcca
     6901 ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg cgcattttta
     6961 ttggccgcaa cggtaacgtc ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac
     7021 gctgccactg cggcaacttt ggctgcctgg aaactatcgc cgccaacgct gccattgaac
     7081 aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg gacgactgca
     7141 ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag tctggcgtcg gaagtaattg
     7201 agtatgtcgg tcgtcatctg ggtaaaacca tcgccattgc tatcaactta tttaatccgc
     7261 aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc cctgctattg
     7321 aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg gtggtacgtt
     7381 ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca
     7441 acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg gcgcttattg
     7501 ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata tcgacggcgt
     7561 gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg ggattatgga
     7621 taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc aagatctggc
     7681 gaaccgcttt gccactgccg gtgtcgatgt gccggatagc gtgttttata cctctgcgat
     7741 ggcgactgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg tgggcgaagg
     7801 cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga accctgattt
     7861 tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag cggcctattt
     7921 cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc gcggttttta
     7981 tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca aaccgttcta
     8041 tgttggtaag cccagcccgt ggatcatccg cgcggcatta aacaaaatgc aggcgcattc
     8101 ggaagaaacg gtgattgtcg gcgataactt gcgcaccgac attctggcgg gcttccaggc
     8161 gggtctggag acgattctgg tgctttctgg tgtttcatcg ctcgacgata tcgacagtat
     8221 gccgttccgc ccaagctgga tttacccgtc ggtcgctgaa atcgacgtta tctgaatagc
     8281 aaccacgtct cagggcgtgg tttcttattt ttacccatca ttaaaatcat cccattcaat
     8341 aaaactcgcc aaaaattatc gatccatcaa taaatacatc accacaccct cccctttttc
     8401 acaaatcagc aatcaccatt acgtttttta tttttgcact gccaaatcgc ttgcgcacgg
     8461 tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat accaggttaa
     8521 cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac gcagttgagc
     8581 tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg
     8641 gtatttatgc cagcgataac gccattctcg cccacgaacg tctgtcaatt gttgacgtta
     8701 acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggcg gtaaacggtg
     8761 aaatctacaa ccaccaggca ctgcgcgccg aatatggcga tcgttatcag ttccagaccg
     8821 gatctgactg cgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg
     8881 acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgca tatctgattg
     8941 gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgagcac ggtcagctgt
     9001 atgtggcctc agaaatgaaa gccctggtgc cagtttgccg cacgattaaa gagttcccgg
     9061 cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat cgcgactggt
     9121 tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt caggcactgg
     9181 aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgctg ctttctggtg
     9241 gtctggattc ctcaattatt tccgctatca ccaagaaata cgcagcccgt cgcgtggaag
     9301 atcaggaacg ctctgaagcc tggtggcccc agttacactc ctttgctgta ggtctgccgg
     9361 gttcaccgga tcttaaagca gcccaggaag tggcaaacca tctgggcacg gtgcatcacg
     9421 aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt taccacatcg
     9481 aaacttatga tgtgaccact attcgcgctt caacaccgat gtatttaatg tcgcgtaaga
     9541 tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg
     9601 gttatcttta cttccataaa gcgcccaacg ctaaagaact gcatgaagag acggtgcgta
     9661 aactgctggc cctgcatatg tatgactgcg cgcgcgccaa caaagcgatg tcagcctggg
     9721 gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta
     9781 acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt
     9841 ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc tccgatggcg
     9901 tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt tctgatcagc
     9961 aactggaaac tgcccgcttc cgcttcccgt acaacacgcc aacctcaaaa gaagcgtatc
    10021 tgtaccggga gatctttgaa gaactgttcc cgcttccgag cgccgctgag tgcgtgccgg
    10081 gcggcccgtc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag gcgttcaaga
    10141 aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa
    10201 tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct tgttgattct
    10261 gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac tcatcactgt
    10321 aggccagtat ttcatagatt aatttcccac caccattgtt gaattccgaa tttcatcctg
    10381 tatgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat aattgacgaa
    10441 tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa
    10501 aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta
    10561 tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact cataatgatg
    10621 gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt ttgcgggagt ggcgaaattg
    10681 gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc
    10741 cgcaccatta accagaaagc gttgtacgga tggggtatcg ccaagcggta aggcaccggt
    10801 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcttc ttcgagtaag
    10861 cggttcaccg cccggttatt ggggtatcgc caagcggtaa ggcaccggtt tttgataccg
    10921 gcattccctg gttcgaatcc aggtacccca gccatcgaag aaacaatctg gctacgtagc
    10981 tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag
    11041 ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt
    11101 atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta
    11161 ccccagccaa tttattcaag acgcttacat cgtaagtgca cccagttggg gtatcgccaa
    11221 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
    11281 acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat
    11341 gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac
    11401 ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg
    11461 cctgacgttt caacacgcca gcacgctccg ccgccattaa cccgagatta cgcataaaac
    11521 gcagtggtgg cagattattg ctgaatccgg cataaaacag atccataccg ctttgcataa
    11581 tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gattggataa ctggcccacg
    11641 attcgccgta gctgcgggcg ttgaccagaa catcaatcag ggcatcgaca tcacgataac
    11701 caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg
    11761 caagccctgg ctgcacatac tgcaacgcat ggcgacgcgt cagcggaaac gcaccagcgg
    11821 caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctgtaact
    11881 gcgccatgtt catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg
    11941 atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggagta aattgctgcc
    12001 aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatacac gactgcgcat
    12061 actgccaggc atgaacgcca atacccgcca tctgccgcac ctgcgaattt gcgccgtcgg
    12121 caccaatcac cagcttcgcg cggatcgttt ctccaccctt cagttccagc tcctgaagat
    12181 catcatggcg atgcagcgca atcagtgagc ctggcacacg taacgttact ttcggatgcg
    12241 cttccagcgc ctgccacagc gcctgttgca ggacggtgtt ttccaccata tagccaagca
    12301 gcggcagctt aagttcagcg gcgtcaaaca tcacatgcgc cgtttcccac tcccacgttt
    12361 ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt
    12421 taagcaatga taccgatgcg gcgctgatcg ccgagatccg cacgtccggt tggctatcag
    12481 cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca
    12541 gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt
    12601 gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc
    12661 ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc
    12721 tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa
    12781 cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca
    12841 cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct
    12901 gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt
    12961 taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag
    13021 gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc
    13081 accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca
    13141 gcttcccgga aatcgagaag tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg
    13201 cgtttgtctc catcatggaa ggctgcaata aatattgtac ctactgcgtg gtgccttaca
    13261 cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg
    13321 ctgctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa cgtgaacgcc tggcgtggtg
    13381 aaaactacga cggcaccacc ggatcgtttg ccgatctgct gcgtctggtt gctgcgatcg
    13441 acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca
    13501 tcgaagtgta ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacaaagcg
    13561 gttccgatcg cattctgaac ctgatgggcc gtactcacac ggcgctggag tacaaagcga
    13621 tcatccgtaa actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg
    13681 gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca
    13741 atttcgacat gagctacagc tttatcttct ctgcacgtcc ggggacgccc gccgccgata
    13801 tggtggatga tgttccggaa gaagagaaga agcagcgtct gtatattctg caagagcgca
    13861 ttaatcagca agcgatggcg tggagccgcc ggatgctcgg caccacccag cgtattctgg
    13921 tagaaggtac atcgcgtaag agcatcatgg agctttccgg gcgtacggaa aataaccgcg
    13981 tggtcaactt cgaaggcacg ccggatatga tcggtaaatt cgtcgatgta gaaattaccg
    14041 acgtctaccc gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc
    14101 gcatggcaga aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg
    14161 tgggttatta tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct
    14221 ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct
    14281 tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat
    14341 agacactcgc gaaatcaccc tggagccagc agacaacgcg cgtctgttga gcctgtgcgg
    14401 cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg
    14461 cgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct
    14521 gcgtagcctg tatgtcgata cagccccgat gcgcggtcag attcaggata tcgaaccgga
    14581 acagatccac cttgcgatta aagaagcgcg agtactggag caaagtgcgg agagcgtgcc
    14641 ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc
    14701 aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc
    14761 ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca
    14821 ggagattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt
    14881 cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt acgacgcgct
    14941 gtttgaaatg ctgggctttg agaaagttga gaaactgatt gagcgcaacg ttattgaagt
    15001 cgcgccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga
    15061 gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc
    15121 aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg
    15181 cttacgtcac gccatcgaag tgctggccga tgtcgaagag atcagcttta acttcttcca
    15241 cagcgaagac gtggttcgtc accctgtggt ggcgcgtatc gttaacgcct atgaagcctg
    15301 ggaagaagcc gaacaaaaac gaaaagcggc gctggcggca gaacgcaagc gcgaagaaca
    15361 ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg
    15421 ttaccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa
    15481 gaatcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct gaatctgacc
    15541 tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc
    15601 atggaaatgt cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct
    15661 caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg
    15721 catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga agccctcgaa
    15781 acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt
    15841 gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta
    15901 actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga
    15961 taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga
    16021 aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg
    16081 aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca
    16141 tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc
    16201 ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc
    16261 acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag
    16321 ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag
    16381 accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat
    16441 tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactaatt gttggtgaga
    16501 ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct
    16561 ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta
    16621 gcgatgaaga ggttgacact atcggtggtc tggtgatgca ggcatttggg catcttccgg
    16681 cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg gccgacagtc
    16741 ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagccg aagctggatg
    16801 aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc
    16861 gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt
    16921 acgacgtctg gcctgcggcg attatttcgt tgatggggct tcaggcgttg acctttaacc
    16981 gccgtccact ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg
    17041 gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca
    17101 tcttcctggt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg
    17161 tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gccccttctc
    17221 tctggcaagt gaccgagttt ctgcgcggtt gggtactgac aggcttcccg tggttacagt
    17281 tcggctatag ccagattgat ggcccgttaa aagggctggc accgataatg ggcgtggaag
    17341 ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca
    17401 actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca
    17461 tccaatggtt taccccacaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata
    17521 ttccgcaatc actgaaatgg gatggagatc agcttcttaa tacgctgaag atttactaca
    17581 acgcaacggc tccgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa
    17641 ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgcgata
    17701 aaggtagctc gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg
    17761 atacctacaa caccatcatc acactgggta aaggtgcacc gtacagctac gaatcagccg
    17821 atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc
    17881 tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata
    17941 tccagccgcc gctgtcggta aatggtattg agcttactgc ggccatttgc tacgagatca
    18001 ttcttggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg ctgactatct
    18061 ccaacgatgc gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa
    18121 tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg
    18181 tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttga
    18241 ccactaacgt gacgccgacc accggactca caccgtacgc acgaaccggc aactggccgc
    18301 tgtgggtgct gacggcactg tttggttttg ccgctgtgct gatgagtctg cgagcacgta
    18361 aacgttgatg tgcctgatgt atcctgcatg agcaaaacgt actttctcat tgagctttgg
    18421 ggcatggtta tcatgcccct ttttcttacg gttcagggat acaagccaca ttgccatatt
    18481 ggggatgacg aataattgcc tgcggatcaa gcgagcgcgt cctgtccagt tggatcaacc
    18541 cgttcttcgc cataacaaat atttttctac catccgctga aaaacgaagg tgattaacgt
    18601 agctggcagg ataaacgcca ataatctgcc cggcattaat attgagcagg atgacataac
    18661 cttgcaggcc atatgacacc aacgctaaat actgaccgtc cgggctaata ccctcaacgg
    18721 aataaacgct cctgctacac cattgcgtca taatgctggc ttgttgatcc cctacgctta
    18781 tacgtgaaat cccctggctg tcggcaaaaa ttacatcccc gttttcaggt cgaaaacgca
    18841 tcgccgatat cgttcctcgc gcatcactag ccggatagct ttctttctga tgcgtgatgg
    18901 tattgatgcg tacaatgtca cctctgacca taccgggtat caacaatatt ttttcatccg
    18961 gggaaagagc cataacccag gcaccaggtt tattactcgt ccccgtcccg tcgtatttct
    19021 ccagcgggat atccgccaca tgctgataag tcaccagatc aatcgctgac acactattac
    19081 tggtgcgatt catcacccag gcttttgtgc catcacgcga gaggataagg ctgtcaggat
    19141 gtgcaccggt ggtaatcgac tgaataatct cccctgtcgc cacgttaatg accgataacg
    19201 cattcgcttc aaatgaagtc gcataaatca acttttcatc cgggcttaat gccagcgatc
    19261 tgacgccctg atctttacag ccaccacaat gtttgactga cttttctttc gctatgggcg
    19321 ctgtaaaggt atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg ctgttcgtgt
    19381 tttcaattgc ggcatacaac atattaccat cccgactgat cgccatatca tggatatcgg
    19441 caatctcagc gagcaaatca ccactggcct gttcacgact taccggagcg gataatgttt
    19501 ccctgagggc gttgcgttcc tccgctaact gctgcttttt ttgccgtcgc tcttgttcaa
    19561 tctcctgtgg ataaaacgct tcacgcaggc tttgcctgac ttgtaaaact gactcatcca
    19621 tctgctgctg cgcaagccag atagccccgg tcagaagaag actgcccgca acaataatcc
    19681 cgccttccgt aacgagtcgg ttttgcccag ataaacgcgc gctcagccaa cccaacgcgc
    19741 cacccgcaat caggccgcca atgtgacaag cattatctat tcctgtttgc cgggcaccat
    19801 acaacagtgt cagcgccacc atacccaaca gagaaaatac tcgccgctgg tttttatggt
    19861 atgtgcccgc acctgaagca aactgtgttg ctatcgctgc accagctatt cccagaatcg
    19921 ccccggatgc cccaacgctg atgactatct gctcactttc gcgtaacgca taacaggcgc
    19981 tgatgagccc accaaagacg ccggagaata accaggtaat caggagtcgc catttcccca
    20041 gaatctgttc cgccacgcct cccacgacca ggaatgcaaa catattcatc agcagttgag
    20101 aaacactact gtgcagaaag atactgctca ataagcgcca gggttcaccc gacaaagtaa
    20161 gaggcgcaac gtttgcaccg agatggataa gtgcggattg atcgacctct tcataactca
    20221 tccattgtgc gagataacaa aatatcccaa tacagagtgc aaaaaggata agggttattg
    20281 cgggtaattg aacatttaac ggcttaaccg aagatgcaga cataaggtgt ttcttccttg
    20341 aaaagacagg taaaaagcat ttttcccgtg tgcagttatc atcccggcaa acgtcccatg
    20401 ccgaaaagat aattctgtcc gaaatatgac tttttcagcc taaaattaca atcgtaatta
    20461 attcgcccct aagtattttt tggcatgtaa gttgctttat ttactgtgta agcgatgaaa
    20521 tcagccgcga cgcaaccagg ttgcaccacc acaaatcagc ggtagcacca aaaaggtgcg
    20581 gtttaagaat tttgcttcgg aatggtgcgg cgcatctcgc aaaaacaaac aataaaacag
    20641 caatatcttt acatttagcc aaactaaatg ttacctaaca tgcacaacac tgcacaataa
    20701 agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga
    20761 agataaagga gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc
    20821 tttccgcagg actggcacag gcagatgacg ccgccccggt agcgggcagt acgctggaca
    20881 aaatcgccaa aaacggtgtg attgtcgtcg gtcatcgtga atcttcagta cctttctctt
    20941 attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg
    21001 aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct
    21061 cacaaaaccg tattccgctg ctgcaaaacg gcacttttga ttttgaatgt ggttctacca
    21121 ccaacaacgt cgaacgccaa aaacaggcgg ctttctctga cactattttc gtggtcggta
    21181 cgcgcctgct gaccaaaaag ggcggcgata tcaaagattt tgccgacctg aaaggcaaag
    21241 ccgtagtcgt cacttccggc actacctctg aagttttgct caacaaactg aatgaagagc
    21301 aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc cgcaccctgg
    21361 aaagcggtcg tgccgttgcc tttatgatgg atgacgctct gctggccggt gaacgtgcga
    21421 aagcgaagaa accagacaac tgggaaatcg tcggcaagcc gcagtctcag gaggcctacg
    21481 gttgtatgtt gcgtaaagat gatccgcagt tcaaaaagct gatggatgac accatcgctc
    21541 aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa aatccaattc
    21601 cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag
    21661 aaccgaatga caaggcactg aactaattac aagaaccagg ggcggaaatt ccagccctct
    21721 cgattgttac gtagcacgga cagactatac gcctgatggt cgttccccat cgggcctgaa
    21781 aaccgcaata cgctgggtaa caatcttcga gggtagcagt taacgctgct accctctttt
    21841 ttctggagta gatttatgtc tatagactgg aactggggta tttttttaca acaagccccg
    21901 ttcggcaaca ccacctatct tggttggatc tggagtggtt ttcaggtgac gatcgctttg
    21961 tcgatctgcg cctggattat cgctttcctc gtcggttcat ttttcggcat tttacgtacc
    22021 gttccaaacc gttttctctc cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg
    22081 ccgttgattg tgcaattctt tacctggtat ctggtgatcc cggagctgtt gccggagaaa
    22141 atcggcatgt ggtttaaggc cgagctggat cccaatattc agtttttcct ttcatccatg
    22201 ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag
    22261 tcgctcccgc gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct accgcaggct
    22321 taccgctatg tactgctgcc taatgcttat cgcgttatcg tcccgccgat gacctccgag
    22381 atgatgaacc tggtgaaaaa ctccgccatc gcctcgacta tcggtctggt ggatatggca
    22441 gcgcaggcgg gtaaattgct cgactactcg gctcacgcct gggagtcgtt taccgccatt
    22501 acgctggcgt atgtattgat taacgcggtt atcatgctgg tgatgacgct ggttgaacgt
    22561 aaagttcgcc tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca
    22621 ttgttccttc cctgccatat ctgctcgacg ggctggtgat cacgctgaaa atcaccgtca
    22681 cggcagtcgt gattggtatt ttgtggggca ccatgctggc ggtgatgcgc ttgtccagct
    22741 ttgcgcccgt agcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag
    22801 ttatggtttt gctgtggttt tacctgatcg tgccgggttt tctgcaaaac gtgctgggat
    22861 tatcgccaaa aaatgatatc cgtttaattt cggcgatggt ggcgttttcg atgtttgaag
    22921 cggcctacta ttcagagatt atccgtgctg gtattcaaag tatttctcgt ggtcagtcga
    22981 gcgccgcgct ggcgttggga atgactcact ggcagtcgat gaaactgatt attctgccgc
    23041 aggcgttccg cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata
    23101 cctcactggt gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg
    23161 agcgtgatgg tacgcaggtc gagatgatcc tgtttgccgg atttgtttat ttcgttatta
    23221 gccttagcgc gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct
    23281 gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt
    23341 gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt tccggaaaat caacgctgat
    23401 taaaaccgtc aacggcctcg aaccggtgca gcaaggtgaa atcaccgtcg atggtatcgt
    23461 ggttaacgac aagaaaaccg atctggcaaa gctgcgttcc cgagtcggga tggtgttcca
    23521 gcattttgag ttgttccctc atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa
    23581 agtgcttaaa cgcgataaag cgccggctcg tgaaaaagca ttgaaattgc tggagcgcgt
    23641 gggactttcc gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcagcagcg
    23701 tgtggcgatc gctcgcgcat tgtgtatgga tcctattgcg atgctgttcg acgaaccgac
    23761 gtcggcgctg gatccggaga tgatcaacga agtgctggac gtgatggtgg aactggcgaa
    23821 cgaaggaatg accatgatgg tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa
    23881 tcgggtgatc tttatggatg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt
    23941 cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact
    24001 ctggcgcgca tacccaagtg cgcgccatct cacgctttat cgtttcgccc ctcgtatctg
    24061 ttacatcctg gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact
    24121 gccaattctg ttagattgcg acccaggtca tgacgacgct atcgcaatag ttctcgccct
    24181 cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga
    24241 aaaaacctta cgcaatgttc ttcgtatgct gaccttgctc aatcgcaccg atattccggt
    24301 agcaggcggc gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg
    24361 cgaaagcggt ctcgacggcc cggcattacc ggaaccggcg ttcgcaccgc aaaactgtac
    24421 ggcggtagag ctaatggcga aaacgctgcg tgaaagtgcg gaacctgtca cgattgtgtc
    24481 taccggaccg caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa
    24541 aattgcccgt atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc
    24601 tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc
    24661 ggtggtgatg gccggtctgg atgttactca caaagcacaa atccacgttg aagacactga
    24721 gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt
    24781 cctcgaatat cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg
    24841 caccatcgcc tggctgttga aaccggagtt gtttaccact gttgagcgct gggttggcgt
    24901 ggaaacacag gggaaatata ctcagggtat gacggttgtt gattattatt atctgactgg
    24961 caataaaccg aatgccaccg taatggtcga tgttgatcgt cagggctttg ttgatttact
    25021 ggctgatcgt ctgaaatttt acgcttaaca cacgtcattg attaagatca ccacgcgtta
    25081 cctgggtggt cttaatctgt ttatctcttt ttctttcaag atataaatgc aatggataat
    25141 gcagaactcg ccattggtat cgatctcggt actaccaata gtttaattgc cgtctggaaa
    25201 gacggtgccg cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt
    25261 agcatggatg aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg
    25321 catccggata aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg
    25381 ttaggcagcg acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa
    25441 gaagatgccg aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct
    25501 tatttcagcg atgaacaacg caagcatacc cgtttagcag cggagttagc cgggttaaat
    25561 gcggtacgct taattaatga acccacagca gctgcgatgg catatggcct gcatacgcaa
    25621 caaaataccc gctcgctggt ttttgatctc ggtggcggca cgtttgacgt cacggtgctc
    25681 gaatacgcca cgccggtgat tgaagttcac gcctccgctg gcgataactt cctcggtggc
    25741 gaagatttta cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc
    25801 acgcttaacg agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc
    25861 aatcaatcgc cattgcacat tcgctggcag taccaagatg aaacgcggga gtgcgaattt
    25921 tacgagaacg aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt
    25981 gaacaggcgt tgcgcgatgc acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt
    26041 ggcggcgcgt cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta
    26101 ccgtatcaaa gttacgaccc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc
    26161 tgccgcttac gcagtgaaga tattgaagag gtgatcctca ctgatatttg cccttactcg
    26221 ttgggcgttg aagttaaccg ccagggcatt tccggcattt tctcgccaat tattgaacga
    26281 aacaccacag taccggtgtc gcgtgtagaa acttactcga ccatgcaccc ggaacaggat
    26341 tctattacgg ttaacgtcta tcagggagaa aaccacaaag ttaaaaataa cattctggtt
    26401 gaatccttcg atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt
    26461 agttatgata tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag
    26521 tccagagtga ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg
    26581 acgcggttat ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa
    26641 gccaaactgg aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg
    26701 gtgatcaccg attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc
    26761 aggcggcggg cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg
    26821 gcgtaaatgc tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg
    26881 attttgccga gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta
    26941 atgctctgtg gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca
    27001 tcgacatctt catcctccgg ctcgagtttt actttcgtca acagcaaccg ggtgataatg
    27061 gcctctaatg gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct
    27121 tgtttcggaa acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt
    27181 gaatcttctt ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg
    27241 aagaataacg actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt
    27301 tccgcccata acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct
    27361 tgcgcatcta ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg
    27421 caatgacgct tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactctcc
    27481 cagtatgcgc agagctcagc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca
    27541 cactggtaat accgggcatc ttcgctttct ggttcttccg cttgccactg tttggctaca
    27601 ggaatgagtt ccgccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt
    27661 aactttgcat catcaggaat gaaccacgga ccttccatcg ccaaaaacgc agtgacatag
    27721 tcaggatggt aacggcagca acgttccagc gcgaagtaat attcaacagt ctggttctgt
    27781 aacgcgacgg gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc
    27841 tcaagaaaag cctccctttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag
    27901 ttcaaacgct ctgccagcaa agaagcacaa tttaatgaaa gatatcgcgc ttctatagcg
    27961 attgcacaca gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc
    28021 tgaataaatt tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc
    28081 ctgaatgcac gtagtatttc atgttcagcg gcggcatctt tatcttcatt atctgcttcc
    28141 ccgacaggat ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac
    28201 ccctgcggat cggtttcggg atgacataac ggtaagcgag caagataagc ctggcggata
    28261 atgtctattt gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc
    28321 tcacatcctt aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg
    28381 tggcatagaa acgatcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat
    28441 cgtatttgta cagggttagc gcctcgcgta gcgaagtgtt gtcttcgcac tgagaagatt
    28501 gccagtagag acgtcctaac gaataggcgg aaaaatagtg catccagctg tgatagtaat
    28561 ggtgagctcg tacatagacg cgcgattgca gccataaact ctcttcttcc gtcagccagt
    28621 tgttgagcac accgacccgt gacagaaacc ccattcgaac ataatcccag gctttgattc
    28681 ccccttctcc acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct
    28741 ccgagccacc agcaattaat gctttccact cttcagggga agagcgatgc catgtcagat
    28801 ataagccagc taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta
    28861 aatcatcacg attatttatt ccccaggatg atgagagatc gaattctttt ataccggtat
    28921 acatttttgg cgagtcatgc gcgggtttgc aaccctgatt aagtatttcc atgggcgcag
    28981 aaagcgcaaa aagaagatat tgttcttcct tattcatttg ttaataatcc cttaagtgca
    29041 ttaact
//
LOCUS       DAMZII010000055        28290 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZII010000055 DAMZII010000000
VERSION     DAMZII010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.055"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="POA77_002312"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="POA77_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6365741.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="POA77_002313"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="POA77_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI6365742.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="POA77_002314"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="POA77_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI6365743.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="POA77_002315"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="POA77_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI6365744.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="POA77_002316"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="POA77_002316"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDI6365745.1"
                     /translation="MLHTLHRSPWLTDFAALRRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="POA77_002317"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="POA77_002317"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDI6365746.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="POA77_002318"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="POA77_002318"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDI6365747.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="POA77_002319"
     CDS             complement(4598..5320)
                     /locus_tag="POA77_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6365748.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="POA77_002320"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="POA77_002320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6365749.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="POA77_002321"
     CDS             6520..6738
                     /locus_tag="POA77_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDI6365750.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="POA77_002322"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="POA77_002322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6365751.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="POA77_002323"
     CDS             complement(7472..7672)
                     /locus_tag="POA77_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDI6365752.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="POA77_002324"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="POA77_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDI6365753.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="POA77_002325"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="POA77_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDI6365754.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="POA77_002326"
     CDS             10169..12082
                     /locus_tag="POA77_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6365755.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="POA77_002327"
     CDS             12082..13104
                     /locus_tag="POA77_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6365756.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="POA77_002328"
     CDS             13098..13316
                     /locus_tag="POA77_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDI6365757.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="POA77_002329"
     CDS             13370..14239
                     /locus_tag="POA77_002329"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDI6365758.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="POA77_002330"
     CDS             complement(14294..14698)
                     /locus_tag="POA77_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI6365759.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="POA77_002331"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="POA77_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDI6365760.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="POA77_002332"
     CDS             15683..17773
                     /locus_tag="POA77_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6365761.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="POA77_002333"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="POA77_002333"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDI6365762.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQSLLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="POA77_002334"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="POA77_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDI6365763.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /locus_tag="POA77_002335"
     CDS             complement(19741..20343)
                     /locus_tag="POA77_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDI6365764.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="POA77_002336"
     CDS             complement(20333..20500)
                     /locus_tag="POA77_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDI6365765.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="POA77_002337"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="POA77_002337"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDI6365766.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="POA77_002338"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="POA77_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDI6365767.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="POA77_002339"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="POA77_002339"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDI6365768.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="POA77_002340"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="POA77_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI6365769.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="POA77_002341"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="POA77_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDI6365770.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cobA"
                     /locus_tag="POA77_002342"
     CDS             26709..28082
                     /gene="cobA"
                     /locus_tag="POA77_002342"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6365771.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6856 a         7598 c         7132 g         6704 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 accacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gccgcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcatttact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 acacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgacctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 cgcgccaaaa gagaatgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcatccc catgcgcggc tttagcaact gccatctaca aactatgctg
    12181 ccgcgtctgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct tgcgtggagt gaaaaccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcacgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatga ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg cagccgtcac gccgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcactgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg acgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcataatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggagaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccacgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggct tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggccacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtacc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaacatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaagcg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggaa cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctct cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactacgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacgta gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28261 acgagcagtc tgcttatatt catataatca
//
LOCUS       DAMZII010000056        28014 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZII010000056 DAMZII010000000
VERSION     DAMZII010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.056"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            370..2793
                     /gene="pgaA"
                     /locus_tag="POA77_002343"
     CDS             370..2793
                     /gene="pgaA"
                     /locus_tag="POA77_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDI6365772.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2802..4820
                     /gene="pgaB"
                     /locus_tag="POA77_002344"
     CDS             2802..4820
                     /gene="pgaB"
                     /locus_tag="POA77_002344"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDI6365773.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4813..6138
                     /gene="pgaC"
                     /locus_tag="POA77_002345"
     CDS             4813..6138
                     /gene="pgaC"
                     /locus_tag="POA77_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDI6365774.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6140..6553
                     /gene="pgaD"
                     /locus_tag="POA77_002346"
     CDS             6140..6553
                     /gene="pgaD"
                     /locus_tag="POA77_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDI6365775.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6603..7391)
                     /gene="phoH"
                     /locus_tag="POA77_002347"
     CDS             complement(6603..7391)
                     /gene="phoH"
                     /locus_tag="POA77_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDI6365776.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8011..9282)
                     /gene="efeB"
                     /locus_tag="POA77_002348"
     CDS             complement(8011..9282)
                     /gene="efeB"
                     /locus_tag="POA77_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDI6365777.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9288..10415)
                     /gene="efeO"
                     /locus_tag="POA77_002349"
     CDS             complement(9288..10415)
                     /gene="efeO"
                     /locus_tag="POA77_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDI6365778.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10473..11303)
                     /locus_tag="POA77_002350"
     CDS             complement(10473..11303)
                     /locus_tag="POA77_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDI6365779.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11845..13353)
                     /gene="putP"
                     /locus_tag="POA77_002351"
     CDS             complement(11845..13353)
                     /gene="putP"
                     /locus_tag="POA77_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDI6365780.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13512..13721)
                     /locus_tag="POA77_002352"
     CDS             complement(13512..13721)
                     /locus_tag="POA77_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365781.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13776..17738
                     /gene="putA"
                     /locus_tag="POA77_002353"
     CDS             13776..17738
                     /gene="putA"
                     /locus_tag="POA77_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI6365782.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17778..18416)
                     /gene="rutR"
                     /locus_tag="POA77_002354"
     CDS             complement(17778..18416)
                     /gene="rutR"
                     /locus_tag="POA77_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDI6365783.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18704..19795
                     /gene="rutA"
                     /locus_tag="POA77_002355"
     CDS             18704..19795
                     /gene="rutA"
                     /locus_tag="POA77_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDI6365784.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19795..20487
                     /gene="rutB"
                     /locus_tag="POA77_002356"
     CDS             19795..20487
                     /gene="rutB"
                     /locus_tag="POA77_002356"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDI6365785.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20499..20885
                     /gene="rutC"
                     /locus_tag="POA77_002357"
     CDS             20499..20885
                     /gene="rutC"
                     /locus_tag="POA77_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDI6365786.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20893..21693
                     /gene="rutD"
                     /locus_tag="POA77_002358"
     CDS             20893..21693
                     /gene="rutD"
                     /locus_tag="POA77_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDI6365787.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21703..22293
                     /locus_tag="POA77_002359"
     CDS             21703..22293
                     /locus_tag="POA77_002359"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDI6365788.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22304..22798
                     /gene="rutF"
                     /locus_tag="POA77_002360"
     CDS             22304..22798
                     /gene="rutF"
                     /locus_tag="POA77_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDI6365789.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            22819..24147
                     /gene="rutG"
                     /locus_tag="POA77_002361"
     CDS             22819..24147
                     /gene="rutG"
                     /locus_tag="POA77_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDI6365790.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24230..24403)
                     /locus_tag="POA77_002362"
     CDS             complement(24230..24403)
                     /locus_tag="POA77_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6365791.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24776..25372
                     /gene="wrbA"
                     /locus_tag="POA77_002363"
     CDS             24776..25372
                     /gene="wrbA"
                     /locus_tag="POA77_002363"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI6365792.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25393..25620
                     /locus_tag="POA77_002364"
     CDS             25393..25620
                     /locus_tag="POA77_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDI6365793.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25658..26899)
                     /gene="agp"
                     /locus_tag="POA77_002365"
     CDS             complement(25658..26899)
                     /gene="agp"
                     /locus_tag="POA77_002365"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDI6365794.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGAMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27192..>28014)
                     /locus_tag="POA77_002366"
     CDS             complement(27192..>28014)
                     /locus_tag="POA77_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365795.1"
                     /translation="NITACDDVFSEYCLDDENMSIQPEQINTPGISDLDTGIDLSGIS
                     FIQSEINKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGGHKTQKQVYEEFIKIVD
                     KQTIEALVDTAFYNLREDVKTLFGVNLQSDNKIQGQSSLMSWSFLFFKKQFIDSCLNN
                     EKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSICSLFIVNSNMASISL
                     GNESFSTDDDLEYGYLINAGNHYDVYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT         6892 a         7210 c         7019 g         6893 t
ORIGIN      
        1 tcatagatac attaggaata acaattaaat ccgtgagtgc cgtagcgcag cctttcatca
       61 ggactttcga aaaatccgaa atcatgcatc ggaatttact gatttaatta ttttaatcct
      121 aatttatttt gaaaaaggca ttgggattta tgccgtattc ctgaagatcc tcatcattgg
      181 aatggatttt cgggcgagaa aaggatttta tatggacact ctgctcatca tttcttcttc
      241 tcatcatcaa caattcacgt ctctcttccg cgtttaataa cggattatga ggtgcaaaaa
      301 tatctttctt ttcagttacc tgtaattaga tacagagaga gattttggca atacatggag
      361 taatacagga tgtattcaag tagcagaaaa aggtgcccga aaaccaaatg ggctttgaaa
      421 cttcttactg ccgcattttt agcagcgagt cccgcggcga agagtgctgt taataacgcc
      481 tatgatgcat tgattattga agctcgcaag ggtaatactc agccagcttt gtcatggttt
      541 gcactaaaat cagcactcag caataaccaa attgctgact ggttacagat tgccttatgg
      601 gccgggcaag ataaacaggt tattaccgtt tacaaccgct accgtcatca gcaattacca
      661 gcgcgtggtt atgcagctgt cgccgtcgct tatcgtaacc tgcaacaatg gcaaaactcg
      721 cttacactgt ggcaaaaggc gctctctctg gagccgcaaa ataaggatta tcaacgggga
      781 caaattttaa ccctggcaga tgctggtcac tatgatactg cgctggttaa acttaagcag
      841 cttaactctg gagcaccgga caaagccaat ttactcgcag aagcctatat ctataaactg
      901 gcggggcgtc atcaggatga attacgggcg atgacagagt cattacctga aaatgcatct
      961 acgcaacaat atcccacaga atacgtgcag gcattacgta ataatcaact tgctgccgcg
     1021 attgacgatg ccaatttaac gccagatatt cgcgctgata ttcatgccga actggtcaga
     1081 ctgtcgttta tgcctacgcg cagtgaaagt gaacgttatg ccattgccga tcgcgccctc
     1141 gcccaatacg ctgcattaga aattctgtgg cacgataacc cagaccgtac tgcccagtac
     1201 cagcgtattc aggttgatca tcttggcgcg ttattaactc gcgatcgtta taaagacgtt
     1261 atttctcact atcagcgatt aaaaaagacg gggcaaatta ttccgccctg ggggcaatat
     1321 tgggttgcat cggcttatct caaagatcat cagccgaaaa aagcacagtc aataatgacc
     1381 gagctctttt atcacaagga gaccattgcc ccggatttat ccgatgaaga acttgcggat
     1441 ctcttttaca gccacctgga gagtgaaaat tatccgggcg cgctaactgt cacccaacat
     1501 accattaata cttcgccgcc tttccttcgg ttaatgggca cgcctacgag catcccgaat
     1561 gatacctggt tacaggggca ttcgtttctc tcaaccgtag caaaatatag taatgatctt
     1621 cctcaggctg aaatgacagc cagagagctt gcttataacg caccaggaaa tcagggactg
     1681 cgcattgatt acgcgagtgt gttacaagcc cgcggttggc ctcgtgcagc agaaaatgaa
     1741 ttaaaaaaag cagaagtgat cgagccacgt aatattaatc tggaggttga acaagcctgg
     1801 acagcattaa cgttacaaga atggcagcag gcagctgtct taacgcacga tgttgtcgaa
     1861 cgtgaaccgc aagatcccgg cgttgtacga ttaaaacgtg cggttgatgt acataatctt
     1921 gcagagcttc gtatcgctgg ctcaacagga attgatgccg aaggcccgga tagtggtaaa
     1981 catgatgtcg acttaaccac catcgtttat tcaccaccgc tgaaggataa ctggcgcggt
     2041 tttgctggat tcggttatgc cgatggacaa tttagcgaag gaaaagggat tgttcgcgac
     2101 tggcttgcgg gtgttgagtg gcggtcacgt aatatctggc tcgaggcaga gtacgctgaa
     2161 cgcgttttca atcatgagca taaacccggc gcgcgcctgt ctggctggta tgattttaat
     2221 gataactggc gtattggttc gcaactggaa cgcctctctc accgcgttcc attacgggca
     2281 atgaaaaatg gtgttacagg caacagtgct caggcttatg ttcgctggta tcaaaatgag
     2341 cggcgtaagt acggtgtctc ctgggctttc actgattttt ccgacagtaa ccagcgtcat
     2401 gaagtctcac ttgagggtca ggaacgcatc tggtcttcac catatttgat tgtcgatttc
     2461 ctacccagtc tgtattacga acaaaataca gaacacgata ccccatacta caaccctata
     2521 aaaacgttcg atattgttcc ggcatttgag gcaagccatt tgttatggcg aagctatgaa
     2581 aatagctggg agcaaatatt cagcgcaggt gttggtgcct cctggcaaaa acattatggc
     2641 acggatgtcg tcacccaact cggctacggg caacgcatta gctggaatga cgtgattgat
     2701 gctggcgcaa cgctacgctg ggaaaaacga ccttatgacg gtgacagaga acacaactta
     2761 tacgttgaat tcgatatgac attcagattt taaggataaa tatgttacgt aatggaaata
     2821 aatatctcct gatgctggtg agtataatta tgctcaccgc gtgcattagc cagtcaagaa
     2881 catcatttat accgccacag gatcgcgaat ctttactcgc cgagcaaccg tggccgcata
     2941 atggttttgt agcgatttca tggcataacg ttgaagacga agctgccgac cagcgtttta
     3001 tgtcagtgcg gacatcagca ctgcgtgaac aatttgcctg gctgcgcgag aacggttatc
     3061 aaccggtcag tattgctcaa attcgtgaag cacatcgagg aggaaaaccg ctaccggaaa
     3121 aagctgtagt gctgactttt gatgacggct accagagttt ttatacccgc gtcttcccaa
     3181 ttcttcaggc cttccagtgg cctgctgtat gggcccccgt cggcagttgg gtcgatacgc
     3241 cagcggataa acaagtaaaa tttggcgatg agttggtcga tcgagaatat tttgccacgt
     3301 ggcaacaagt gcgagaagtt gcgcgttccc ggctcgttga gctcgcttct catacatgga
     3361 attctcacta cggtattcag gctaatgcca ccggcagctt attgcctgta tatgtaaatc
     3421 gtgcatattt tactgaccac gcacggtatg aaaccgcagc agaataccgg gaaagaattc
     3481 gtctggatgc tgtaaaaatg acggaatacc tgcgtacaaa ggttgaggta aatccacacg
     3541 tttttgtttg gccttatggc gaagcgaatg gcatagcgat agaggaatta aaaaaactcg
     3601 gttatgacat gttcttcacc cttgaatcag gtttggcaaa tgcgtcgcaa ttggattcca
     3661 ttccgcgggt attaatcgcc aataatccct cattaaaaga gtttgcccag caaattatta
     3721 ccgtacagga aaaatcacca caacggataa tgcatatcga tcttgattac gtttatgacg
     3781 aaaacctcca gcaaatggat cgcaatattg atgtgctaat tcagcgggtg aaagatatgc
     3841 aaatatcaac cgtgtatttg caggcatttg ctgatcccga tggtgatggg ctggtcaaag
     3901 aggtctggtt tccaaatcgt ttgctaccaa tgaaagcaga tatttttagt cgggttgcct
     3961 ggcaattacg tacccgctca ggtgtaaaca tctatgcgtg gatgccggta ttaagctggg
     4021 atttagatcc cacattaacg cgagtaaaat acttaccaac aggggagaaa aaagcacaaa
     4081 ttcatcctga acaatatcac cgtctctctc ctttcgatga cagagtcaga gcacaagttg
     4141 gcatgttata tgaagatctt gccggacatg ctgcttttga tggcatattg ttccacgatg
     4201 atgctttgct ttcagattat gaagatgcca gtgcaccggc tatcacggct tatcagcaag
     4261 caggctttag cgggagtctg agcgaaattc gacaaaaccc ggagcaattt aaacagtggg
     4321 cccgctttaa aagtcgtgcg ttaactgact tcactttaga acttagtgcg cgcgtaaaag
     4381 ccattcgcgg tccacatatt aaaactgcac gaaatatttt tgcacttccg gtaatacaac
     4441 ctgaaagtga agcctggttt gcacagaatt atgctgattt cctaaaaagc tatgactgga
     4501 ccgctattat ggctatgcct tatctggaag gtgtcgcaga aaaatcggct gaccaatggt
     4561 taatacaatt gaccaatcaa attaaaaaca tccctcaggc taaagacaaa tctattttag
     4621 aattacaggc acaaaactgg cagaaaaatg gtcagcatca ggctatttct tcgcaacaac
     4681 tcgctcactg gatgagccta ttacaactga atggagtgaa aaactatggt tattatcccg
     4741 acaattttct gcataaccaa cctgaaatag accttattcg tcctgagttt tcaacagcct
     4801 ggtatccgaa aaatgattaa tcgcatcgta tcgtttttta tattatgtct ggtgttatgc
     4861 atacccctat gcgtagcgta ctttcactct ggtgaactga tgatgaggtt cgttttcttc
     4921 tggccgtttt ttatgtccat tatgtggatt gttggcggcg tctatttctg ggtctatcgt
     4981 gaacgccact ggccgtgggg agaaaacgca ccagctcccc agttgaaaga taatccgtct
     5041 atctccatta tcattccctg ttttaatgag gagaaaaacg ttgaggaaac catacacgcc
     5101 gctttagcac agcgttatga gaacattgaa gttattgccg taaatgacgg ttcaacagat
     5161 aaaacccgtg ccatcctgga tcgcatggct gcacaaattc cccatttgcg ggtcattcat
     5221 ctggcgcaaa accaggggaa agccattgcg cttaaaaccg gagctgccgc ggcgaaaagt
     5281 gaatatctgg tgtgcattga tggcgatgcg ttattagacc gcgatgcggc ggcatatatt
     5341 gtggaaccga tgttgtacaa cccgcgtgtg ggtgccgtaa ccggtaatcc tcgtattcga
     5401 acacgttcta ccctggtggg taaaattcag gttggcgagt attcctcaat tattggtttg
     5461 atcaagcgaa cccagcgtat ctatggaaac gtatttaccg tttccggtgt tattgccgca
     5521 tttcgtcgca gcgccctggc agaagtgggt tactggagtg acgatatgat caccgaagat
     5581 attgatatta gctggaagct gcagttgaat cagtggacga ttttttacga gccacgggcg
     5641 ctgtgctgga tattaatgcc tgaaacgtta aaagggctgt ggaaacagcg cctgcgctgg
     5701 gctcagggcg gtgcagaagt attcctcaaa aatatgacaa ggttgtggcg caaagaaaac
     5761 tttcgaatgt ggccgctgtt ttttgaatac tgcctgacga caatatgggc cttcacctgc
     5821 ctggtcggtt tcattattta cgcagtccaa cttgccggtg taccgttaaa tattgaattg
     5881 acacatatcg ctgcgacaca tactgccgga atattattgt gtacgttatg tttactgcaa
     5941 tttattgtca gcctgatgat cgagaatcgc tatgagcata atctgacttc atcgcttttc
     6001 tggattattt ggttcccggt tattttctgg atgctgagcc tggcaacgac attggtatca
     6061 tttacacgag tcatgttgat gcctaaaaag caacgcgccc gttgggtaag tcccgatcgc
     6121 gggattctga gaggttaata tgaacaattt aattattacg acccgacaat caccagtacg
     6181 tttactggtt gattatgttg ccacaaccat cttgtggaca ttatttgcgt tgttcatatt
     6241 cttattcgcc atggatctgc tgacgggtta ttactggcaa agcgaggcca gaagccgact
     6301 tcagttctat tttttgctgg cagtggcgaa tgccgtcgtg ttaattgtct gggcgctgta
     6361 caataagctg cgttttcaaa aacagcagca tcatgcagcc taccaatata cgccgcaaga
     6421 atatgcagag agcttagcaa tacctgatga gctctatcag caactacaaa aaagccatag
     6481 gatgagcgta cacttcacca gccaggggca aataaaaatg gttgtttcag aaaaagcgct
     6541 agtccgggca taaacaccca aaacaaagcc cggttcgccc gggctctgca ccgataatac
     6601 acttaactgt atgcatgcag cgtacgttgg caaagtgccg aacgtacgca atcctctttt
     6661 ccgaaccgga cgatcccaac catttcatct tcttcgaaac gttccagcgc gtcacttaat
     6721 ccggagcaca cgccgcgagg caaatcgcat tgcgtgatat caccgttgac gataaccgtc
     6781 acgttctccc cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac attctgcgcc
     6841 tcgtcaagaa tgacgactgc attttcaaag gtacgtccac gcatataggc gaacggcgca
     6901 atttccacct tccctatttc cggtcgcagg cagtactgca taaaggaagc ccctaagcgc
     6961 cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc gatatctcca
     7021 ggtaagaagc caagatcttc atcggcttgc agaactggac gggtgacgat aatcctgtcg
     7081 acatccttat gtatcagggc ttctgccgct tttgctgcgc tgatccaggt ttttccgcac
     7141 ccggcttcgc ccgtggcgaa tatcagctgc ttactctcaa tagccttcag atagtgcaat
     7201 tgcgcttcat ttcgcgcgag gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg
     7261 gcttctacgc cgcccatctg cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg
     7321 ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac gagctttgat cactgctttt
     7381 tgtcttccca tggagagcac cttgagttgt ttgtattcat cacacgcgcc gttggcagcg
     7441 cgattatgcg cacgaacatc agagggttgg cttccttgta agccatagtt tgcttttgga
     7501 taaaatgccg aaaaacggct acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa
     7561 ggatgaggtt gaaaatgcaa agtgacgaga tgactaccgg aggagaaaac tccgcgagtg
     7621 gtggcgcgtt gattatctaa aacatgtcca gtacaggacg ttaccatccg cgatctccat
     7681 agtgactgac tatcactgcc gggaacttcc gctgctactt aataagtaca acagatctcg
     7741 catttattgc aacaatatat ttacttatat ttaactataa aacaccattt cagtgacatt
     7801 agtttctact ggaaagatga cagagtgatg acagatatga aaaaagctgt gtgctttcag
     7861 caggatttgc atcaggcatc agtgcacgat cgccgtatgc ggcgtgaacg ccttatccgc
     7921 ctaccgaacc cgcaccgatt gtaggcataa gaagacgcgc aagcatcgca tcaggcatct
     7981 gcgcaagaac gccttatccg tctaaaaaca ttaaacccgc aataacgcgc ttccgagata
     8041 atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa ccgccgccga taggtttaac
     8101 gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc ctttttccag
     8161 atcgtgttgg taacagacaa acagtagccc catatccagt tgcccggagt tggtgacgcc
     8221 cagtgaataa ctgtagccac gacgcagcat caggctggac tcactctccg ccgtgcgggg
     8281 attcgccagc cggatatggc tgtccagcgc aatcaccttc ccttccggat cgctggcgta
     8341 atcgggaaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct tatcacgacc
     8401 aaaaatcgtc tgctgttctt tcagcggagt tctgtcccaa aattccactc gaaactgaat
     8461 caagcgtact gcctgatagc tgccaccgat tgtccacgct ggctcctgct gatctgccgt
     8521 tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg cagtgccgtc
     8581 tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgccg cgtgatcgga
     8641 aataaaccct tcccgcttcc agcgcacgct gagcaaatcc ggcgtatgtt tgatgatatc
     8701 gcgcagtgca tggatcaccg tgtcctgggt gttggcgcaa atttgtagca acacatcacc
     8761 atgacataac gccgcatcca gcgagtcgtt gggaaaacgc gtcatcttct gcaacttttt
     8821 cggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca ccgataacgt
     8881 gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga gtggtggcag
     8941 gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt
     9001 caacaagcga aacaaccgtt caagatcggc tttatcgctg gcaagcacat caaacgccac
     9061 cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac cataaaacgg
     9121 ctgtttctca ttgcgtgcat ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc
     9181 atgagcgacc ggacaacttc ccgccagcgc cagcgcacca atgcctttca gtaaacgtcg
     9241 gcgtgacggt tcattcacgc cgtttttatc ttcatactgc ataatgctta atccaatccc
     9301 agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggtcc tttcagtgca
     9361 ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt acggtatttc
     9421 gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccaacag ttccgggttg
     9481 gctttttgca gttgtggacg cagcagatcg acaattttct gcgagccttc aacgttagcc
     9541 tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctgat tttgctggct
     9601 gccacttcct caatcagtcc ggctgcaccg ccgaccactt ttgaaggtgg gaaagccagt
     9661 tcactgatgc gtttttgcaa atcgaccaca tcggtataaa gctggtcagc gtactgatcc
     9721 atgcctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa accggtgaat
     9781 tttggatcag cggctttttg ctcgtaatca tcttcacggg cgtcaatgct gccatccaga
     9841 tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt cggcgcatac
     9901 agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg
     9961 gtcaccagct gcgtggtttc cgccatgaca tacgctttat atgcagtaat tgcaccacca
    10021 agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt gacgatcaac
    10081 ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg ctgtaaattc
    10141 gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac caccatcacg
    10201 cctttgagga tctcccactc cagcgccttc tggctgtggt tctgaataat gaactgtgtt
    10261 ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatcggtcac ggtcaccttg
    10321 acctgcggca cattagcggc gttagccata aaagcagaag agaacagcgc agccacgctc
    10381 aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct ttaaagtatg
    10441 ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct
    10501 gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa ccagacggcg
    10561 acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac
    10621 agcgagtgag ttgagagcac cgcactcata tcgaaggcga tttcctgaaa gtggttccac
    10681 aatccggctt catgaaatgc gcgaatggca ccagctgcca gccctgcggc gacgaagaga
    10741 ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc gccccagtag
    10801 agcaggaagc ctagcaccac ggcagtagca agaccgagca ttgcacccag cggcggccag
    10861 atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag cccttccctt
    10921 gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc acgctgcaat
    10981 gcgctatcga ctgcctgctc cagttgtact ttgacgttgc gcgacacttt gcgcatccag
    11041 aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc aaacagttcc
    11101 tgctcttttt gcggaaattc gccggtggtt tcgttaatga agatccccaa gcccaggcac
    11161 aacgcagcgg caagcaacac gccaatccac atcacaccaa tccatcggcc tcgctgggta
    11221 cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac
    11281 ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga gtcaatctat
    11341 gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac ggtaaagaaa aatacaagcg
    11401 gaatgtttat aaaatgatgt tgaaatgtaa acaaagagag gtaatgccag gggatataaa
    11461 cgattattcc aggtacaaag atgggattgt ttacaacgag ccatgtgttc ttgccggatg
    11521 ccgcgtaaac gccgtatcca gccgacaaaa ctgtccagac tcaatgcatc agagcttcca
    11581 tgtaaaactg ataagcgggt gtatcaagca atttcgtcca tccgcgatta tcaaatctaa
    11641 ctttttgccc ggaacataat aacagctcgg gtgttggtgg cgtcaaaatc gctgaagcgt
    11701 tccccgaagg ccgggacagc agggggcggc ggcgattggc cgccccctgc gcgctccctg
    11761 caccattcaa tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa
    11821 caaaaccgcc gcaggctaag tcccttagct ttcctgcaac cgtgacggcg gagccgaatg
    11881 atagtgcgca tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag
    11941 caaactaaac actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtagag
    12001 tcccagccag ccgaactgct tccagacgat aaccgtcagc gcaccgatga tcatccccgc
    12061 cagcgcaccg ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa
    12121 cgccgcgcca aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt
    12181 tgccgccagc gcaatcgcca ccagcgccac caccagcacc atcacacgcc ctacccacac
    12241 cagctctttc tggctggcat gtttacgcag aaacgctttg tataaatctt cggtaatcgc
    12301 actggagcac accagcagct ggcagcttaa cgttgacatt accgccgcca gaattgccga
    12361 cagcagaatc ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg
    12421 ctcggcgttc tggtttaccg cgcctgctac cgccgggtgc tcattaaagt aagcaatccc
    12481 aaagaagccg acagccaccg cccctgccag gcagaggatc atccaggtca tactgatacg
    12541 acgcgcatgg acaatgctgt ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg
    12601 ctgcccgaag taacccagcc cccaacccat cagtgagata atggcaacaa agttcagacc
    12661 tttgagcata tcaacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa
    12721 gccaccgaca ctgataatga cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct
    12781 ggcctgtaca gtatcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat
    12841 cgtcgccgca gctcccgccc acagagccgt ttcgtagctc atgccaaagg tactttcaaa
    12901 cagacgcgcg cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac
    12961 cagcgcggag ataatgcgca aaatgcggct tttatcttca aagcgcccgg tgaaataatc
    13021 cggcagggtt aaggcgttat tgttgtattc ggtatgcaca cgcaaccgcc cggccaccag
    13081 cttccagttg atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc
    13141 ggaaagaaaa accgcgcccg gcaaccccat taacagccag ccgctcatat ccgacgcacc
    13201 cgccgataat gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa
    13261 gtttttcgtt gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca
    13321 aaatgtcacc aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg
    13381 caatgtcgaa acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta
    13441 ccagggcgac cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt
    13501 tacattaaat tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt
    13561 tttgcggttg cacctttcaa aaatgttaac tgccgcagag aaaaagtctg agttattttt
    13621 ttaatccctg tcatatcgat ttcttttatt aacatttcat tcatttttaa gcttgctacg
    13681 caggtcacat ttaacatggt tgcacaaagt tgcaacatca tggatatttc acgataacgt
    13741 taagttgcac ctttcagaac aacaggagta atggcatggg aaccaccacc atgggggtta
    13801 agctggacga cgcgacgcgt gagcgtatta agtctgccgc gacacgtatc gatcgcacac
    13861 cacactggtt aattaagcag gcgatttttt cttatctcga acaactggaa aacagcgata
    13921 ctctgccaga gctacctgcg ctgctttctg gcgctgccaa tgagagcgat gaagcaccga
    13981 ctccggcaga ggaaccacac cagccattcc tcgactttgc cgagcaaatt ttgccccagt
    14041 cggtttcccg cgccgcgatc acagcggcct atcgccgccc ggaaaccgaa gcggtttcta
    14101 tgctgctgga acaagcccgc ctgccgcagc cagttgctga acaggcgcac aaactggcgt
    14161 atcaactggc cgataaactg cgtaatcaaa aaaacgccag tggtcgcgca ggtatggttc
    14221 aggggttatt gcaggagttt tcgttgtcat cgcaggaagg cgtggcgctg atgtgtctgg
    14281 cggaagcgtt gttgcgtatt cccgacaaag ccacccgtga tgcgttaatt cgcgacaaaa
    14341 tcagcaacgg taactggcaa tcacacattg gtcgtagccc gtctttgttt gttaatgccg
    14401 ccacctgggg gctgctgttt actggcaaac tggtttccac ccataacgaa gccagcctct
    14461 cccgctcgct gaaccgcatt atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg
    14521 atatggcgat gcgcctgatg ggcgagcagt tcgtcactgg cgaaaccatc gcggaagcgt
    14581 tagccaatgc ccgcaagctg gaagagaaag gtttccgtta ctcttacgat atgcttggcg
    14641 aagctgcact gactgccgca gatgcacaag cgtatatggt ttcctatcag caggcgattc
    14701 acgccattgg taaagcgtcc aatggtcgtg gcatctatga aggtccgggc atttcaatca
    14761 agctgtcggc gctgcatccg cgttacagcc gcgcccagta tgaccgggta atggaagagc
    14821 tttacccacg tctgaaatca ctcaccctgc tggcgcgtca gtacgatatt ggtatcaaca
    14881 ttgacgccga agaggccgat cgtctggaga tctccctcga tctgctggaa aaactctgtt
    14941 tcgagcctga gctggcaggc tggaacggta tcggttttgt tattcaggct tatcaaaaac
    15001 gctgcccact ggtgattgat tacctgattg atctcgccac ccgcagccgt cgccgtctga
    15061 tgattcgcct ggtgaaaggc gcgtactggg atagtgaaat caagcgtgcg cagatggacg
    15121 gccttgaagg ctatccggtt tatacccgca aggtgtatac cgacgtttca tatctcgcct
    15181 gtgcgaaaaa gctgctggcg gtgccgaatc taatctaccc gcagttcgcg acgcacaacg
    15241 cccatacgct ggcagcgatt tatcaactgg caggtcagaa ctactatccg ggtcagtacg
    15301 agttccagtg cctgcatggt atgggcgagc cactgtatga gcaggtcacc gggaaagttg
    15361 ccgacggcaa acttaaccgt ccgtgtcgta tttatgctcc ggtcggtacc catgaaacgc
    15421 tgttggcgta tctggtgcgt cgcctgctgg aaaacggtgc taacacctcg tttgttaacc
    15481 gtattgccga cacctctttg ccactggatg aactggtcgc cgatccggtc actgctgtag
    15541 aaaaactggc gcaacaggaa gggcaaactg gattaccgca tccgaaaatt cccctgccgc
    15601 gcgatcttta cggtcacggg cgcgacaact cggcagggct ggatctcgct aacgaacacc
    15661 ggctggcctc gctctcttct gccctgctca atagtgcact gcaaaaatgg caggccttgc
    15721 caatgctgga acaaccggta gcggcaggtg agatgtcgcc cgttattaac cctgcggaac
    15781 cgaaagatat tgtgggcttt gtgcgtgaag ccacgccgcg tgaggtagaa caggcgctgg
    15841 aaagtgcggt taataacgcg ccaatctggt ttgccactcc tccggttgaa cgcgcggcga
    15901 ttttgcatcg cgctgccgtg ctaatggaaa gccagatgca gcaactgatt ggtattctgg
    15961 tgcgtgaagc cggaaaaacc ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt
    16021 ttctccacta ctatgccgga caggtgcggg atgatttcgc taacgaaacc caccgtccat
    16081 tagggcctgt ggtgtgtatc agtccgtgga acttcccgct ggcgattttc accgggcaga
    16141 tcgctgccgc actggcggca ggtaacagcg tgctggcaaa accggcagaa caaacgccgc
    16201 tgattgccgc gcaagggatc gccattttgc tggaagcggg tgtaccccct ggcgtggtgc
    16261 aattgctgcc aggtcagggg gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc
    16321 gcggggtgat gtttaccggt tcaaccgaag tcgctacgtt actgcagcgc aatatcgcca
    16381 gccgcctgga cgctcagggt cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg
    16441 cgatgattgt cgattcttca gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg
    16501 cgttcgacag tgcgggtcag cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga
    16561 ttgccgacca cacgttgaaa atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc
    16621 cgggtcgcct gaccaccgat atcggtccgg tgattgatag cgaagcgaaa gccaatattg
    16681 agcgccatat tcagaccatg cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa
    16741 acagcgaaga tgcccgtgaa tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac
    16801 tggatgactt tgccgaattg caaaaagagg tctttggtcc ggtgctgcat gtggtgcgtt
    16861 acaaccgtaa ccagctacca gagctgatcg agcagattaa cgcttccggc tatggtctga
    16921 cgcttggtgt ccatacgcgt attgatgaaa ccatcgccca ggtcactggc tcggcccatg
    16981 ttggtaacct gtatgttaac cgtaatatgg tgggcgcagt ggtgggtgtg cagccattcg
    17041 gcggcgaagg gttgtccggt accgggccga aagcaggcgg tccgctctat ctctaccgtc
    17101 tgctggcgaa tcgcccggaa agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt
    17161 atccggtcga tgcacagttg aaagccgcat tgactcagcc gctaaatgca ctgcgggaat
    17221 gggcagcaaa tcgtccagaa ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg
    17281 caggaacaca acgattgctg ccggggccga cgggtgaacg caacacctgg acgctgctgc
    17341 cgcgtgagcg cgtgttgtgt attgccgatg atgagcagga tgcgctgact cagctcgccg
    17401 ccgtgctggc ggtgggcagc caggtactgt ggccggatga cgcgctgcat cgtcagctag
    17461 tgaaggcatt gccatcggca gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa
    17521 ccgctcaacc gtttgatgcg gtgatcttcc acggtgattc ggatcagctt cgcgcattgt
    17581 gtgaagccgt tgccgcgcgg gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg
    17641 aaagcaatat ccttctggaa cggctgtata tcgaacgttc gctgagtgtg aataccgctg
    17701 ccgctggcgg taacgccagt ttaatgacga tcggttaaac aggtagccgg aggatgttac
    17761 aacctcctcc ggcatcttta acgtggtcga atcccctcaa taataatccg ctgcacgttt
    17821 tcaaccgttt gattgaaaaa tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc
    17881 tgaggggcga aatcggcgta atgttgagtg gaagcccaaa tcataaaaat caaatgctgc
    17941 ggatcaatcg gcgcgagttt gccgcttttg acccaaccgg caatcagcgc cgatttctca
    18001 tcaattaatg ccttcaaatc gcccgtcagt tcatccatta acagcggcgc gcctgccagc
    18061 atctccatac agaacaggcg cgaagcctgc ggataatcgc gggagacttc cagcttcaga
    18121 cggatgtact cttttatcgc cgccagcgga gcgaaatctt cacgaaacgc ttttaacggt
    18181 gccagccaga tatcgagaat ctgccgcagc acggcaatat acagcgcctc ttttgacgga
    18241 aagtaataca gcagattggt ttttgaaaca cccgccaact ctgcgatctg ctccagcctt
    18301 gtgccgtgaa aaccgaattg tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc
    18361 ttcgcgctta ctgcgcgcga acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc
    18421 tctcccctct tgtagattgg caacaggata gcaaaacccc cgcccggcct cgaccttctg
    18481 cactctcatc gcgcacagat gcatgtttta tgtgcaactg ttttgaccgt ttagtccact
    18541 ttttaccaga tattttagac aacccaatgt taacacatta ataacattca ctttttaaaa
    18601 actggcatcc gctttgcaaa caagccatta cgcggctgaa aaggatatgc aggatgcagc
    18661 gccccgtctg actttcactt tacgagacga agagaggttg atgatgaaaa ttggcgtatt
    18721 cgtacctatt ggcaacaacg gctggctcat ttcgacccac gcgccgcagt acatgccgac
    18781 ctttgaactg aataaagcca ttgtgcaaaa agcggagcac taccatttcg atttcgccct
    18841 gtcgatgatc aaactgcgtg gctttggcgg caaaactgag ttctgggatc acaaccttga
    18901 gtcgttcacc ttgatggcgg ggctggcggc cgtcacctcg cgcattcaga tttacgccac
    18961 tgctgccacc ttaacgttac ctccggcaat cgtcgcccgt atggccgcaa ccatcgactc
    19021 catctctggc gggcgttttg gcgtcaacct cgtgactggc tggcaaaagc ccgagtatga
    19081 gcagatgggt atctggcctg gcgatgacta tttctcccgt cgttacgact atctcaccga
    19141 atatgttcag gtgctgcgcg acctgtgggg cacggggaaa agcgatttta aaggcgattt
    19201 tttcaccatg aatgattgtc gcgtcagtcc gcaaccgagt gtccccatga aagtgatctg
    19261 cgccgggcaa agcgacgctg gcatggcgtt ctccgcccag tatgccgatt tcaacttctg
    19321 tttcggcaaa ggcgtaaata cgcccacggc tttcgccccg accgctgcgc ggatgaaaca
    19381 ggccgcagag caaaccgggc gcgacgttgg ttcttatgtg ttgtttatgg tgattgccga
    19441 tgaaaccgac gatgccgctc gcgccaaatg ggaacactac aaagcgggcg cggatgaaga
    19501 ggcgttaagc tggctaaccg aacaaagtca gaaagatacc cgctccggta ctgataccaa
    19561 cgtccgtcag atggccgatc ccacttcggc ggtaaacatc aatatgggga cgttagtcgg
    19621 ttcttacgcc agtgtcgcgc gcatgttaga tgaagtcgca agcgtgcctg gtgccgaagg
    19681 cgtgctgttg accttcgatg attttctgtc gggaatcgaa accttcggcg agcgcattca
    19741 accactgatg cagtgccgcg cccatctacc tgcgctgact caggaggtgg catgatgaca
    19801 accttaaccg ctcgaccgga agccattacc ttcgatccgc agcaaagtgc gctgatcgtg
    19861 gtggatatgc aaaacgctta tgccacgcca ggcggctact tagatctcgc cgggtttgat
    19921 gtctcaacca ctcgcccggt cattgccaac attcaaaccg ccgtgaccgc agcgcgagcg
    19981 gcagggatgc tgatcatctg gtttcaaaat ggctgggatg aacagtatgt cgaagctggc
    20041 ggacccggct caccgaattt tcataaatcg aacgccctga aaaccatgcg taagcagccg
    20101 cagctgcagg ggaaattgct ggcgaaaggc tcctgggatt atcaactggt ggatgaactg
    20161 gtgccgcagc ctggcgatat tgtgctgccg aagccgcgct acagcggttt cttcaataca
    20221 ccgctggaca gcattttgcg cagccgtgga atacgccatc tggttttcac cggtatcgct
    20281 accaacgtct gcgtcgaatc gacgctacgc gatggctttt ttctggagta tttcggcgtg
    20341 gtgctggaag acgcaacaca ccaggcgggg ccggaatttg cacagaaagc cgcgttgttc
    20401 aatatcgaaa ccttttttgg ctgggtcagc gacgtcgaaa cgttctgcga cgcgctttct
    20461 cccacatcct ttgctcgtat cgcttaagga gtttaacgat gccaaaatcc gtaattattc
    20521 ccgctggcag cagcgcaccg ctggccccct tcgttcccgg cacgctggct gatggcgtgg
    20581 tgtatgtctc cggtacgctg gcttttgatc aacataataa cgtgctgttt gccgatgacc
    20641 caaaggcgca aacccgccac gttctggaaa ttatccgcaa ggtgatcgag acggcgggtg
    20701 gcacgatggc ggatgtgacc ttcaacagca tctttattac cgactggaaa aattacgccg
    20761 cgattaacga aatctacgcc gagttttttc cgggtgataa accggcgcga ttctgcattc
    20821 agtgcggact ggtaaaacct gacgcgctgg tggaaatcgc cacaattgcg catatcgcca
    20881 agtgaggccg cgatgaaact ttcactctca cctccccctt atgctgatgc gcccgtagtg
    20941 gtgttgattt cgggtcttgg cggtagcggc agttactggt taccgcaact ggcggtgctg
    21001 gagcaggagt atcaggtagt ctgttacgac cagcgcggca ccggcaataa tcccgacacg
    21061 ctggcagaag attacagtat cgcccagatg gcagcggaac tgcatcaggc gctggtagcc
    21121 gcagggattg agcattacgc ggtggtcggc catgcgctcg gtgcgctggt gggaatgcag
    21181 ctggcgctgg attatcccgc gtcggtaact gtgctgatca gcgttaacgg ctggctacga
    21241 ataaacgccc atacgcgccg ctgttttcag gttcgcgaac gattactgta tagcggcggc
    21301 gcgcaggcat gggtggaagc gcagccgttg ttcctctatc ccgccgactg gatggcggcc
    21361 cgcgcacctc gccttgaggc agaagacgcg ctggcactgg cgcattttca gggcaaaaat
    21421 aatttactgc gtcgacttaa cgccctcaaa cgcgctgact ttagtcacca tgcggatcgc
    21481 atccgctgcc cggtgcaaat catctgcgcc agtgatgatc tgctggtgcc atcagcatgt
    21541 tccagtgagc ttcatgccgc cctgcccgat agccagaaaa tggtgatgcg ctatggcgga
    21601 cacgcctgca acgtgaccga ccccgaaacg tttaatgctc ttttactcaa cgggcttgcc
    21661 agcctgttac atcaccgcga agccgccctg taaggaattg ctatgaacga agccgttagc
    21721 ccaggtgcgc ttagcaccct gttcaccgat gcccgcactc acaacggctg gcgggagaca
    21781 cccgtcagcg atgagacgtt acgggagctt tatgccctga tgaaatgggg gccgacatca
    21841 gctaactgtt ctccggcacg gatcgtgttt atccgcactg tagaaggaaa agaacgtctg
    21901 cgcccggcgc tttccagcgg caatctacaa aaaaccctga ccgcgcccgt caccgctatc
    21961 gttgcctggg acagtgaatt ttatgaacgg ttaccactac tgtttcccca cggtgatgcc
    22021 cgcagttggt ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagctcc
    22081 atgcaggcgg cctatctgat cgtcgcttgc cgtgcgctgg gactggatac cggcccgatg
    22141 tcgggttttg accgtcaata cgttgacgac gctttttttg caggcagcac gctgaagagc
    22201 aatctgctga ttaatatcgg ctatggcgat agcagcaaac tttatgcgcg cctgccacgt
    22261 ctgtcctttg aagaagcctg cgggctgttg taaggagcaa actatgaaca tagttgatca
    22321 acaaaccttt cgcgatgcga tgtcctgtat gggcgcggcg gtcaatatca tcaccacgga
    22381 cggtccagcc gggcgcgccg ggttcaccgc cagcgccgtc tgcagcgtga ccgatacgcc
    22441 gcccacatta ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggtat tcaatgaaaa
    22501 tcgaacgctg tgtgtaaata cactgagcgc cgggcaggag ccgctttcaa atctttttgg
    22561 cggcaaaacg cccatggaac accgctttgc cgccgcccgc tggcagacgg gtgtgaccgg
    22621 atgcccgcaa ctggaagagg ctctggtttc gtttgactgc cgtatcagcc aggtggtgag
    22681 cgtcggcacc cacgacattc tgttttgcgc catcgaagcg attcatcgtc acaccacacc
    22741 ctacgggctg gtgtggttcg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc
    22801 tccttaagga gactgctgat ggcaatgttc ggttttcctc actggcagtt aaaatcgacc
    22861 tctacagaga gcggcgtggt cgcgccggat gaacgattgc cgtttgctca gacggcagta
    22921 atgggcgttc aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg
    22981 gggctggatc ccaatctttc cattttaatg tcggggatcg gcacgttact gtttttcttt
    23041 attaccggcg ggcgcgtccc cagttatctc ggctccagcg cggcttttgt cggcgtggtg
    23101 atcgccgcga cgggctttaa cggtcagggc attaacccga atatcagcat tgcgctgggc
    23161 gggattatcg cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaatcggt
    23221 acgcgctgga ttgaacgact gatgccgccg gtggtgacgg gtgcggtggt gatggcgatt
    23281 ggcctgaact tagcgcctat cgcagtgaaa agtgtatcgg cttcggcctt tgatagctgg
    23341 atggcggtaa tgacggtgct gtgtattggg ctggtggcgg tgtttacccg tggcatgatc
    23401 cagcggttat tgattctcgt aggattaatt gtcgcctgtc tgctgtatgg cgtgatgacc
    23461 aaccttttag ggctgggaaa agccgtcgat tttactctcg tcagccatgc tgcctggttc
    23521 ggtctgccac atttttccac tcctgctttt aatggtcagg caatgatgct gattgcgccc
    23581 gtggcggtga ttctggtggc ggaaaactta ggtcatctca aggcagtcgc cgggatgacc
    23641 ggacgcaata tggacccgta tatggggcgg gcattcgtcg gtgatgggct ggcgacgatg
    23701 ctttctggtt ctgtcggtgg cagcggcgta accacctacg ccgagaatat cggtgtaatg
    23761 gccgtgacaa aagtctactc gacgctggtg tttgtcgctg ctgccgtcat cgccatgctg
    23821 ttgggctttt caccgaaatt tggcgcgctg attcatacca ttccggccgc ggtgattggt
    23881 ggtgcatcaa ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat atgggtacaa
    23941 aaccgtgtcg atttaagcca gaacggtaat ttgattatgg tcgcagtaac cttagtgctg
    24001 ggcgcgggtg attttgctct cacgctgggc ggttttacgt tgggagggat tggtacagca
    24061 acctttggcg cgattttact caatgcgttg ttaagcagaa agttggttga cgttccaccg
    24121 ccagaagttg ttcatcagga gccataaaca gaaaaccgcc gggattaccg gcggtgatga
    24181 ctttttgacg agcctaagaa aaaaatgcat tcgtcattga ttagcccggc tagttgtcgc
    24241 ttttgccgtg actgctcttg ccaccttttt tacctgcttc agatgcgcgc tgcgggtcat
    24301 tcttgaaatt acccccgctg tgctgtccac cttttttacc tgcttctgat gctctttcgc
    24361 ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc cattactgac ctccgtctgg
    24421 gtatagacat catattgcat agaattaacg cggcgtatgg cggctgccat attttcgcta
    24481 cgtgggacta agcttagtga actgacgcgg gcagacccgc gtttggtaat atcctgcaac
    24541 agcttcttag cgagaataga tatcatctca aataattatc cataagccgc tcttatgatt
    24601 aaaaaaacgc gatgagataa aaatgtgtca ttttgcgaca aaattacgtg cttgtgaaag
    24661 ttgttataaa tcaaataagt ggttgtgaaa tttgcactct gaaaaggacg tcttatcttt
    24721 aaataagtgg tagcgaatcg ctacggaata gagataacac gaggagtggt tagaaatggc
    24781 taaagttctg gtgctttatt attccatgta cggacatatt gaaacgatgg cacgcgcagt
    24841 cgctgagggt gcaagcaaag tcgatggcgc agaagttgtc gttaagcgtg taccggaaac
    24901 catgccgccg caattatttg aaaaagcagg cggtaaaacg caaactgcac cggttgcaac
    24961 cccgcaagaa ctggccgatt acgacgccat tatttttggt acacctaccc gctttggcaa
    25021 catgtccggt caaatgcgta ccttcctcga ccagacgggc ggcctgtggg cttccggcgc
    25081 actatacgga aaactggcga gcgtctttag ttccaccggt actggcggcg gtcaggaaca
    25141 aactattact tcaacctgga cgacccttgc gcatcacggc atggtaattg tccccattgg
    25201 ctacgcagcg caggaattat ttgacgtttc acaggttcgc ggcggtacgc cgtacggcgc
    25261 aaccaccatc gcaggcggtg acggctcacg ccagccaagc caggaagaac tgtctattgc
    25321 tcgttatcaa ggggaatatg tcgcaggtct ggcagttaaa cttaacggct aatcttcaac
    25381 aggaggatac gcatgccaac tcaagaagcg aaagctcatc acgtcggtga atgggcatct
    25441 ctgcgcaata cgtcgccgga aatagccgag gccattttcg aagtcgccgg gtacgacgaa
    25501 aaaatggcgg aaaagatttg ggaagaaggt agcgatgaag tgttagtcaa agcctttgct
    25561 aagaccgata aagattcgct tttttggggc gaacagacaa tcgaacgtaa aaacgtttaa
    25621 ccagcgactc ccccgcttct cgcgggggag ttttctgtta tttcaccgct tcattcaaca
    25681 cgctatcaaa tttatccatc gggcagaaac cgttggcatc tatcgggcaa ccgcttaatt
    25741 caagcgtcac acgctgagca ggtgcctgca gggttaacgc atcggcatta cgtaactgtt
    25801 ccgcactctg atacacatat tcaattttca tcaaatcgcg attggctttg ctgtcatgcc
    25861 aacgctggaa aacgattttg ccgccaatcg gcgtgcgttc gttctggtca tgcaactgat
    25921 acggtttgaa atccagcgcc gttaacagag aggcaatgtt ggagtcgtgc ccaaccaaca
    25981 ctgtaatttt cggtgcgctg gtgcgatcgg tgaccagagc tttgtcgata taactgacca
    26041 gcggtttcgc aacattgcgc gccacttccg gtgaggtaaa caggctgtcc tggtagccgt
    26101 ttttaagctt cgacaacacc ttccactgct ggtcagattt gatttctccc caggccacct
    26161 gatccatcgg aaaaccttcg taatattgca aagtaaacgc atctaccagc gagttgccga
    26221 ctttcagcgg cccggaaaca cctggttctt gttgatactt cgcgctaaag gtatttttgc
    26281 catccaccag cgaacactgt tgtttctctt tacaggcagg ggaatcttta tagttaacga
    26341 ttttttccag tagctggtag ctgtcggtaa gctggagttt gctgagctct ttctccattg
    26401 ccgccaccgc ctgttcactg aatgcggcgg aatcatcggt gatcaccggg ttaaaggttg
    26461 ggtccatagc gcccattttt tcctggtgat gcacaggaat atcacacccc gggaatgcgc
    26521 cggtaataaa gaactgtgcg gtcgcaacgg tacgttgcag actattggca taggcataaa
    26581 cggtgtacgg cggcgggcat tcccccgatt tcaccatccc ctgctctgcc agccattcac
    26641 gcatgtaatg gcccatatac acttcgagca cgccaccttt ggtggtgagt tgcccaccgg
    26701 ggacgtccca ttctggccat ttattcggcg tcgactgctc cagcacactg ccattgttcg
    26761 ccagcggcgc acgtaagtta tggcggctca tcatgagcac ttgctgtagc tgatagcctt
    26821 ccggtacggt ttgtgcctga gcgtttgaag cgagtaaaac tatccctgcc acagctgcgg
    26881 cgattagcgt tttgttcatt cctggcacct cttttgttat caatcactaa agagtgtgac
    26941 agaaatatga cttttttccg ggaacaggtt cgcaaaatgg cttgttgaac gcctgttttt
    27001 ctatatacat cttcaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc
    27061 ctcgcgtacg ccgctgataa ttgcacctgt ggctaaacag aatatctgcg gcgttaccaa
    27121 tacagagaca atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata
    27181 tgcctttgcc attaaattgc ataacgattt aaatagtcga gttgcgcatt catttcctta
    27241 ttttttaact tataagcctg agcaaaaagt tcaggaggga ggtaaacatc ataatgattg
    27301 ccagcgttta ttaaataacc atactcaagg tcgtcatctg ttgaaaagga ttcattcccc
    27361 aaggagattg atgccatatt actattaaca ataaataatg agcaaatgct atctgaaaga
    27421 acagcaagaa aattcttcac cgctttaatc ctatccgacg tgtcggtatc taaagcaagc
    27481 aagttcttgt tatcattaaa tataaactcg ggtaaatgca ggatacattt ttcgttattc
    27541 aagcaactat caatgaattg ttttttaaaa aacagaaagc tccatgacat aagactactc
    27601 tgcccctgaa ttttgttgtc agattgtaga ttaacgccaa ataacgtctt tacatcttcc
    27661 ctgagattat agaatgccgt atcaacaaga gcctcgatcg tttgtttatc aacaatttta
    27721 ataaactctt cgtatacttg cttttgcgtc ttatggccgc ccagcgccca tgaatactca
    27781 tgttctttta aaaccagtat ggctcttaac agacaatatc catcgccatc tactggagcg
    27841 tattttaatc catatgtttt attaatttca ctctgaataa aagatatgcc agacaaatca
    27901 ataccagtat ctaaatcaga tattcccggc gtatttatct gttctggctg gatactcata
    27961 ttttcatcat ctaaacaata ctcagaaaaa acatcatcac acgcagtaat gttt
//
LOCUS       DAMZII010000057        27659 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZII010000057 DAMZII010000000
VERSION     DAMZII010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.057"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..106
                     /locus_tag="POA77_002367"
     CDS             <1..106
                     /locus_tag="POA77_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDI6365796.1"
                     /translation="LLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(209..1459)
                     /gene="icd"
                     /locus_tag="POA77_002368"
     CDS             complement(209..1459)
                     /gene="icd"
                     /locus_tag="POA77_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI6365797.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1631..2284
                     /gene="rluE"
                     /locus_tag="POA77_002369"
     CDS             1631..2284
                     /gene="rluE"
                     /locus_tag="POA77_002369"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDI6365798.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2294..2755
                     /gene="nudJ"
                     /locus_tag="POA77_002370"
     CDS             2294..2755
                     /gene="nudJ"
                     /locus_tag="POA77_002370"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDI6365799.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2809..3915
                     /gene="mnmA"
                     /locus_tag="POA77_002371"
     CDS             2809..3915
                     /gene="mnmA"
                     /locus_tag="POA77_002371"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI6365800.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3951..4592
                     /gene="hflD"
                     /locus_tag="POA77_002372"
     CDS             3951..4592
                     /gene="hflD"
                     /locus_tag="POA77_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDI6365801.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4596..5966
                     /gene="purB"
                     /locus_tag="POA77_002373"
     CDS             4596..5966
                     /gene="purB"
                     /locus_tag="POA77_002373"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI6365802.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6134..6805
                     /gene="phoP"
                     /locus_tag="POA77_002374"
     CDS             6134..6805
                     /gene="phoP"
                     /locus_tag="POA77_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDI6365803.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6805..8265
                     /gene="phoQ"
                     /locus_tag="POA77_002375"
     CDS             6805..8265
                     /gene="phoQ"
                     /locus_tag="POA77_002375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDI6365804.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8341..9462
                     /gene="roxA"
                     /locus_tag="POA77_002376"
                     /gene_synonym="ycfD"
     CDS             8341..9462
                     /gene="roxA"
                     /locus_tag="POA77_002376"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDI6365805.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9608..10837)
                     /gene="pepT"
                     /locus_tag="POA77_002377"
     CDS             complement(9608..10837)
                     /gene="pepT"
                     /locus_tag="POA77_002377"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6365806.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11087..12223
                     /gene="potA"
                     /locus_tag="POA77_002378"
     CDS             11087..12223
                     /gene="potA"
                     /locus_tag="POA77_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDI6365807.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12207..13064
                     /gene="potB"
                     /locus_tag="POA77_002379"
     CDS             12207..13064
                     /gene="potB"
                     /locus_tag="POA77_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDI6365808.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13061..13855
                     /gene="potC"
                     /locus_tag="POA77_002380"
     CDS             13061..13855
                     /gene="potC"
                     /locus_tag="POA77_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDI6365809.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13852..14898
                     /gene="potD"
                     /locus_tag="POA77_002381"
     CDS             13852..14898
                     /gene="potD"
                     /locus_tag="POA77_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDI6365810.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14956..15417
                     /locus_tag="POA77_002382"
     CDS             14956..15417
                     /locus_tag="POA77_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDI6365811.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            15414..16190
                     /locus_tag="POA77_002383"
     CDS             15414..16190
                     /locus_tag="POA77_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDI6365812.1"
                     /translation="MKKFSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEIARKA
                     ELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTREPRL
                     IMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPTPPLT
                     YYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTIANMG
                     YQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16329..17150)
                     /gene="cobB"
                     /locus_tag="POA77_002384"
     CDS             complement(16329..17150)
                     /gene="cobB"
                     /locus_tag="POA77_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI6365813.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17166..18077)
                     /gene="nagK"
                     /locus_tag="POA77_002385"
     CDS             complement(17166..18077)
                     /gene="nagK"
                     /locus_tag="POA77_002385"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDI6365814.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18106..19350)
                     /gene="lolE"
                     /locus_tag="POA77_002386"
     CDS             complement(18106..19350)
                     /gene="lolE"
                     /locus_tag="POA77_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDI6365815.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19350..20051)
                     /gene="lolD"
                     /locus_tag="POA77_002387"
     CDS             complement(19350..20051)
                     /gene="lolD"
                     /locus_tag="POA77_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDI6365816.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20044..21243)
                     /gene="lolC"
                     /locus_tag="POA77_002388"
     CDS             complement(20044..21243)
                     /gene="lolC"
                     /locus_tag="POA77_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDI6365817.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21505..22578
                     /locus_tag="POA77_002389"
     CDS             21505..22578
                     /locus_tag="POA77_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI6365818.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22706..26152
                     /gene="mfd"
                     /locus_tag="POA77_002390"
     CDS             22706..26152
                     /gene="mfd"
                     /locus_tag="POA77_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI6365819.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26296..27258
                     /gene="ldtC"
                     /locus_tag="POA77_002391"
     CDS             26296..27258
                     /gene="ldtC"
                     /locus_tag="POA77_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDI6365820.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27341..27598)
                     /gene="bhsA"
                     /locus_tag="POA77_002392"
     CDS             complement(27341..27598)
                     /gene="bhsA"
                     /locus_tag="POA77_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDI6365821.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6763 a         7149 c         6994 g         6753 t
ORIGIN      
        1 tctactcaaa agcagtcaag agtgtcctcc aggagaaagc aaaataacaa ataaatgttt
       61 gcataatagt tgcattataa aaatatgccc atatgggctc aagtaaaggg ttttatataa
      121 atatgctcga tatgctatta ataataccta cataaataaa acaacgggcg tgttatacgc
      181 ccgttgcaat attaaacaca tgtagtgatt acatgttctt gatgatcgca tcaccaaatt
      241 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      301 tcgcattgat tgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      361 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      421 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      481 ttgcaccagg ggcgataccg ataccgccaa cctgcgctgc cagggcgtca gaaatgtagt
      541 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      601 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggtg ttcgggtttt
      661 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaactcttca cgcgccagct
      721 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      781 gggtcacaga gtcacggtcg ttggcaattg cgtattcaat cgcggcacgg accagacgtt
      841 tggtgccttc ttcagaacac ggcttgatac cgataccgca atgttccggg aagcgaattt
      901 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtctg
      961 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
     1021 tcagttcagg gtgtttaacc gggcttggag tgccttgata gtaacgtacc ggacgcaggc
     1081 agatgtagag atccagttcc tggcgcaggg caacgttcag agagcgaata ccgccaccaa
     1141 caggagtggt cagcggacct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1201 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1261 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1321 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1381 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1441 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1501 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1561 gatttgtaat tggcttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1621 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1681 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac ctgaaaacca
     1741 gcccacgcgt gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1801 agccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1861 tcttgaccgc gatagcgaag ggttactggt gctgaccaat aacggcgcgt tgcaggcgcg
     1921 tttaacccag ccgggtaaac gcaccgggaa aatctattat gtgcaggtgg aaggtattcc
     1981 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     2041 gcccgccggc gcggagctgg ttgacgaacc agcgtggtta tggccgcgga atccaccaat
     2101 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2161 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2221 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2281 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2341 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2401 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2461 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2521 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2581 tcatgacagc gatatcgact gctgtcgttg ggtcagcgcc gaagagattt taaaggcgtc
     2641 aaatcttcgt tcgccgctgg tggcggaaag tatccgttgt tatcaaagcg ggcaacgcta
     2701 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2761 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2821 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2881 ttgcaacaac agggatatca ggttgaaggc ctgtttatga agaactggga agaagacgac
     2941 ggtgaggaat attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag
     3001 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     3061 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa
     3121 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3181 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3241 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3301 caaagcctgt tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3361 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3421 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3481 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3541 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3601 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3661 gtcgggttga ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggcactatg
     3721 cgttgcacgg taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg
     3781 gacgatgatc gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag
     3841 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3901 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3961 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     4021 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     4081 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4141 tcgggctgga aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgccg
     4201 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4261 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4321 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4381 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4441 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4501 tcggcggcgg acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4561 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4621 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4681 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4741 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4801 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4861 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4921 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4981 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     5041 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     5101 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5161 accgtatgga gcgccagtac cgccagctta atcaggtgga gatcctcggc aaaatcaacg
     5221 gcgcggtcgg taactataac gcccatatcg ccgcttaccc ggaagttgac tggcatcagt
     5281 tcagcgaaga gttcgtcacc tcgctgggta ttcagtggaa cccgtacacc acccagatcg
     5341 aaccgcacga ctacattgcc gaattgtttg attgcgttgc gcgcttcaac accattctga
     5401 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5461 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatt gacttcgaaa
     5521 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5581 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5641 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5701 taaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gctgagccaa
     5761 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga
     5821 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5881 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5941 tcaccatggt tgatgagctg aaataaactc gtatcagtgc cggatggcga tgctgtccgg
     6001 cctgtttatt aaaattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     6061 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6121 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6181 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6241 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6301 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac tgccgattct
     6361 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6421 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6481 gcggcgtaat agcggcctgg cttcacaggt catttcgcta cccccgtttc aggttgatct
     6541 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6601 cattatggaa acgttgatac gcaataatgg caaagtggtg agcaaagatt cgttaatgct
     6661 ccaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6721 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtg attactaccg ttcgcggcca
     6781 gggctatttg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctca
     6841 ctgcgggtac gttttctgtt ggcaacggct gcggtagtac tggtgctttc gcttgcctac
     6901 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6961 cgtggcgaga gcaatctgtt ctataccctt gcgaagtggg aaaacaataa gttgcatgtc
     7021 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     7081 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaagatgat ccagcctgac
     7141 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7201 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7261 gcggagatga cccactcggt ggcagtaaac gtctacccgg caacatcgcg gatgccaaaa
     7321 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7381 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7441 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7501 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7561 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7621 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7681 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7741 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7801 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7861 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7921 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7981 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     8041 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     8101 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8161 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8221 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccatacttc
     8281 acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac
     8341 atggaatacc aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc
     8401 ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg
     8461 gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa
     8521 tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca
     8581 ttactggtac aggcagtaaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc
     8641 cgtgaactac cggactggcg tattgatgat ctgatgatct ctttttctgt acctggcggc
     8701 ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggacgtcgt
     8761 cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc ggatctgtta
     8821 caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga tattctttat
     8881 attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg
     8941 ggttttcgcg cgccgaatac gcgggaactg attagcggat ttgccgatta tgtgctgcaa
     9001 cgtgaactgg gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg
     9061 gatgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag
     9121 ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat
     9181 atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct acgatgcgct gaaacaaggt
     9241 gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc
     9301 aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt
     9361 gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcaatgctc
     9421 gcggcgctgg taaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga
     9481 tgcggcgctg attgcgcctt atcgtgccta catgcactga acgcctgtag gcacgataag
     9541 tatgttgacc agtatcaata taacggtttc atcccggtaa ttggcagtcc acatacgcat
     9601 cgggcaatca cgacttccgt tgcgccgtta actcggcaat acggacaatc acctgcaccg
     9661 ctttttccat accttccaga gtcacaaact catgcttacc atgatagttg taaccgccag
     9721 tgaacaggtt cgggcacggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc
     9781 ggatcggttt cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga
     9841 taatatgcgg atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca
     9901 gttcaatgta gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt
     9961 tacgtttacg cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg
    10021 ctcggtcaac ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca
    10081 tttccgggct ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat
    10141 ttaccatcac tcctttcgcc gtgcccggat gaacattgtt accgacaatt ttgatattga
    10201 ccgatgcggc gttgaagttt tcaaactcca gttcacctac gccaccaccg tcaacagtgt
    10261 aagcccagcg ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt
    10321 catccggggt aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg
    10381 ccagcgcggt catgatttct gcaataccgg ctttgtcatc ggcacctaac aaggttttac
    10441 catcggtagt aatcagcgtc tgacccagta gctgatgcag caccgggaac ataaccggtg
    10501 ataaaacttc atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt
    10561 gcggattcac atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa
    10621 tcgccgggat attgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac
    10681 ttaaggtcac attgataagc cccatctctt cgagctgctc tttcagtaga tgcaataact
    10741 tccattggcc ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag
    10801 acacgtagtt caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga
    10861 caacattatt aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa
    10921 gcgggaatat ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca
    10981 aagattggcg attcaagcgg tttgccgtag aattaccgcc ctcattaaga gtcaccaagg
    11041 tggttaacca caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa
    11101 aaaattgaat aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa
    11161 atgctttgat ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt
    11221 cctcacgctg cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg
    11281 tctggaaact gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc
    11341 ggcggaaaac cgctatgtga acactgtttt ccaaagctac gcacttttcc cccacatgac
    11401 cgtgttcgaa aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac
    11461 gccccgcgtg atggaagccc tgcggatggt gcaactggaa accttcgctc aacgtaaacc
    11521 gcatcagctc tctggtggtc aacagcaacg cgtcgccatt gctcgcgcgg tggttaacaa
    11581 gcctcgtctg ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca
    11641 aatgcagaac gagctgaaag cgttacagcg taagcttggt attaccttcg tctttgtcac
    11701 tcacgatcag gaagaagcgc tcaccatgtc agacaggatt gtggtgatgc gcgagggtcg
    11761 tattgagcaa gatggtacac cgcgcgaaat ttacgaagag ccgaaaaacc tgtttgttgc
    11821 cggcttcatt ggcgaaatca atatgtttaa cgccactgtc atcgaacgtc tagacgagca
    11881 gcgcgtacgc gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga
    11941 accggggcaa aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa
    12001 cgacgacaac cacgctgaag ggctgattgg ttacgttcgt gagcgtaact acaaaggcat
    12061 gacgctggag tcggttgttg aactggaaaa tggcaagatg gtgatggtca gcgaattctt
    12121 caatgaagac gaccctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt
    12181 agaaagctgg gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga
    12241 ttgtcactat tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg
    12301 gcaccagctt tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt acgctggata
    12361 actacacgcg tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg
    12421 cgctgatcgc caccctcgcc tgcctggtgc tgggctaccc gtttgcctgg tttctggcga
    12481 agctaccaca caaggtgcgt ccgctgctgc tgtttctgct gattgttccc ttctggacca
    12541 actcactgat tcgcatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg
    12601 agtttttgct ctggctgggc gttatcgaca caccaattcg tatcatgttc acgcccagtg
    12661 cggtgattat cggtctggtt tacattctgc taccgtttat ggtgatgccg ctgtactcca
    12721 gtatcgaaaa actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt
    12781 tacagacttt tatccgtatc attattccac tgacgatgcc gggaattatt gccggatgtc
    12841 tgctggtgat gctgccagcg atgggcctgt tctatgtatc cgacctgatg ggcggtgcga
    12901 aaaacctgct gatcggtaac gtcatcaagg tccagttcct caatattcgt gactggccgt
    12961 ttggtgcagc caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact
    13021 ggcgcgcttc tcgtctgctg aataaaaagg tggaactcga atgatcggtc gactgcttcg
    13081 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
    13141 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
    13201 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
    13261 ggtgttttcg gcgacatttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
    13321 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
    13381 agatattgtg atggcgattt ctctgctggt gctgtttatg ctgctgggta ttcagcttgg
    13441 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccgtttgtgg tggtgacggt
    13501 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
    13561 cagtgaattt accattctgc gaaaaatcat tctgccactg gcaatgcctg cggtggcggc
    13621 gggctgggtg ttaagcttta ccctgtcgat ggacgatgta gtagtttctt cgttcgtcac
    13681 cggaccaagc tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
    13741 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggtaattgc
    13801 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
    13861 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
    13921 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
    13981 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
    14041 accatgtacg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
    14101 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
    14161 ttaacgaact tcagcaatct cgatccagac atgctcaaca agccgtttga cccgaataac
    14221 gattactcca ttccgtatat ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg
    14281 gatccgaaat ctgtcaccag ctgggccgat ctgtggaagc ctgagtacaa aggcagcctg
    14341 ctgttgaccg acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc
    14401 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
    14461 ccaaacgtcg cagcgtttaa ctccgataac ccagctaacc cgtacatgga aggcgaagtt
    14521 aacctcggca tgatctggaa cggttctgct ttcgttgcac ggcaggcggg tacgccaatt
    14581 gacgtggtgt ggccgaaaga aggcggcatt ttctggatgg acagcctggc gatcccggca
    14641 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
    14701 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
    14761 ttaagcccgg aagtggcgaa cgataaaacg ctctacccgg atgctgaaac cattaaaaat
    14821 ggtgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
    14881 ctgaaagcag gacgttaatt cagcaagcgg gcggtggcat taccgcccgt tttttattta
    14941 gcaaggatgt ggctaatgag tcaggacagt aaagtgttat cccgcgcttt tctcggtatc
    15001 ggtctgatat tgctcgttat ttctgcaatc atcttttatt accattttac ttttaccaaa
    15061 agcgcggttc atacagaagg aatcattgtt gatgccgttt ggtacaacaa tcactcaaac
    15121 gacgtcgatg ataatggttc ctggtatcca gtggttgcct ttcgccctac gccagattac
    15181 acgctaattt tcaattcgaa tatcggtagt gatttttatg aagatagcga aggtgacaga
    15241 gtcaatgtct attatccgcc agggcatcca gaacaagccg aaataaacaa tccctgggtt
    15301 aattttttta aatggggctt tgttggcata gcaggcatta ttttcggtag tgtcggtctg
    15361 ataatcacgc tcccctccac caaaaaatct cgcaggaaac ggaagtcctg cccatgaaaa
    15421 aattcagttt actgatactc ctgcctctgg tagcaaccag taaaccattg attccaataa
    15481 tgaagacact ttttacggat gttaccggta ctgtaccgga tgctgaagag atcgcacgta
    15541 aagccgagct ttttcgccaa caaacgggaa ttgcaccgtt tattgtgatt ttacccgaca
    15601 ttaataatga agccagtctc agacaaaatg gtaaagcgat gctggcgcat gcgtcatctt
    15661 cattgagtaa tgtaaaagga agtgttctgt tactatttac tacccgcgaa ccacggttaa
    15721 ttatgatcac caacggccag gttgaaagtg gtctggacga taaacatctc ggccttctga
    15781 tagaaaatca cacgctggct tatttaaatg cagatctctg gtatcaggga atcaataatg
    15841 cattggctgt tctacaagca cagatattaa aacaaccgac gccgccactg acgtattatc
    15901 cgcatccagg acaacagcat gaaaatgctc cccccggcag caccaacaca ttaggtttta
    15961 tcgcctgggc tgcgacgttt atcttatttt cctggatctt ttgctacacg actcgtttta
    16021 tttatgcgtt aaaatttgca gtggcgatga ctattgccaa tatgggttat caggctttat
    16081 gtttgtatat cgataatagc tttgcgatta ccagaatatc gccactctgg gcagggctaa
    16141 ttggtgtctg tacgtttatt gccgcattgt tattgacgag taaacgataa aagaggccgg
    16201 aaaagtgcgc gtgtattatg ccggatgggg cgcgaacgcc ttatccggcc cacaaaaccc
    16261 gcaaattcaa ttaattgcgt ccccttgtag gcctgataag cgtagcgcat caggcaatgc
    16321 ttcctgcttt ataatccctt cagcaacttt tcaacaaact caggcaccac ctggcttgcc
    16381 gggccgtaat atttctcggc aaattcatta ccgacctgac ttggttcgag attcagttcc
    16441 acggtgtgcg cgccatgcag tttcgcttcg tgaacaaacc cagccgccgg ataaacatgc
    16501 ccggaagtac caatggcaat gaaaatatca gccatcgaca acgccatata aatttcatcc
    16561 atgccgagtg gcatttcgcc aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg
    16621 caacaatggc atttatcttc tggcgtaacg tctccggtcc agtcgagaac ctgaccactt
    16681 tgcgaacaac gaactttcag caattcccca tgcatatgaa tgacattggt attacctgcg
    16741 cgttcatgca ggttgtctat attctgcgtt accagcaaaa agcgatcgcc gagggcgtct
    16801 tgcagtttag ccagcgcaag atgcgcggcg ttaggctgaa tttctggctg ctgcagctgt
    16861 cgacgacggg cgttataaaa cgcttgcacc agttcaggat cgcgatcgaa accttccggc
    16921 gttgccacat cttcaacccg atgttcttcc cacaggccat ctgcggcgcg aaaggtacga
    16981 atacctgatt ccgcagaaat ccctgcccct gtcagtacga gtactcttgg tttttccatt
    17041 gcttccggca ccactttatc tctgaaaaaa atacgctgac gcaaacgctc gcgcaggcgg
    17101 cgtttatttt tacgaaaacg acttaaccga tgaccccgac gcgacagcat agcaacctct
    17161 gttgtttaat cggttagatg taggaaggcc gcaccacgca ttcctcccgc atcaccgtgg
    17221 cgcgcgcgtt caatgcgcgg aacacgagct acaggtaaga gatgacgagg cagcctgtcc
    17281 gccagttgcg ttgtgattgc cgggaaattc gataagccac caccaatgac gaccaggtca
    17341 gggtcaacaa tggtcaggat atttcccaga caaaccgcta ataaatccag ataacgctca
    17401 acgtgcgccc ttgcctgctc atcgccttga tcataaagcg caataatttc gggagcctgc
    17461 aacggttgat gataatagtg ttgatacagc cacgcaaaac cgcgaccaga cagataattt
    17521 tcaatgcagc catgctgacc acagccgcag cggcgtaacg ggaaatccaa ccccatcatg
    17581 gttaacgcat caaccggcag acgcatatgg ccaaactcgc cggtaatgta gcttttaccg
    17641 gtaatcggtt tgccgttgaa aatcagcccg ccgccaacgc cggtgccgag aatcaacccc
    17701 atcaccagtg gatattgagt aaattcatca tcccaggctt ctgaaagggc aaaacagttg
    17761 gcatcgttat caaggcgtac atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt
    17821 ttaccgctgg cagcggggac attggcggca tacagcgtac catcttctgt ttccggcatt
    17881 cccggaatac cgatgccgac agaaccttta cagccaaaac gttggtcagc ttcagctacc
    17941 agctcacaca ctgcatctaa aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt
    18001 tcccactgca actgccgacc gctatcaaac acgccaagcg caatttttgt tccaccaata
    18061 tcaaacccgt aatacattgc cgctccttgt tttaatgtac tgcctttact ggccgctaag
    18121 gactcgcgca gggtcaatat tactggcgcg ccgcgccgga taccaacttg ccaaaagact
    18181 cagcaacaat gctgtgacca gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag
    18241 gaaatcaata aaatagatat cgctggagag gaactgatga ccgatcaact tttcaatcca
    18301 ctcaataatc ggggtaagtt gcagtgaaac caccacaccg ataatcaccc cacacaggct
    18361 gccgaacagc cctgccagca atccatacca gacaaagatg gcgcgaatta aaccatcttt
    18421 cgcccccagc gttcttaata ctgcgatatc gccactcttg tctttcaccg ccatcactaa
    18481 ggtggagacg atgttgaaac aggccacgcc aatcaccagt accatcgcca gatacataat
    18541 ggcgcggatc atctggatat cgcgatacat atagccgtaa gtaccaatcc aacttttaat
    18601 ataaacatag ctgttggtca cttcacccgc atcgcgtacc agcttattgg cgttgaaaac
    18661 atccgtcatt ttaagggcaa tacctgacac gctggaaccc atatcaagat attgttgggc
    18721 atccgccagc gggatcatgg caaaactgtg atcgagttga ccactcaact gcaaaatacc
    18781 ggcaacgtgc aaacgcacac gttttggctg catcagttta tgctcgggat tcgagttggg
    18841 gatcataatc gacacccaat ccccctgctt cactttcagc gcatccgcca cgcctttgcc
    18901 gatgataatt tgctgttcgc ccgctttaaa attgcgccac gcatcgccct ggacaaacga
    18961 gggtaatgcg ctcagacgct gttcctgttg cgggttaacg cccttcacct ggattgcgcg
    19021 aagattcgcg ccactttcca ccagcccggt gaaattgata tacggcgcgg cagcggcaat
    19081 acccggcact ttttgcacgt gatccagtgc ttcctgccag ttagtccacg gctgatccac
    19141 cgcctcgatt tcaccatgcg gcaccaccgc cagaatacgg ttgttcagtt cgcgttcaaa
    19201 gccgttcatc gcgcttaagc cgacgatcaa taccgccacg ccgagggcaa tgccaatggt
    19261 agaaataacg gagatcagcg acaccatgcc gccgcgccgc cgtccgcgac taaaacgcag
    19321 gccaattaat aacgataaag gcatcgccat tactccgccc ccatcaggct cagttccgcc
    19381 gtcagacgac catcacgcat ttctaactgg cggctcatac gtttcgccag ttgcaggtcg
    19441 tgagtaacca ccaggaaggc ggtgccctgc aagcgattca attccccaag caactggaag
    19501 atgctgtcgg cgttacgcgc atcgaggtta ccggtaggtt catccgccag taccaggcgc
    19561 gggttattca ccagcgcacg ggcaatcgcc acacgctggc gttcgccgcc agaaagttca
    19621 gatgggcggt gattcgcacg atgatccagc cccaccgctt ttaacatctc aagtgcacgg
    19681 ctgttgattt cagcgggctt tttcttgcca atcagcagcg gcatagccac gttttccagg
    19741 gcggtaaaat ccggcagcag gtggtgaaac tgataaataa agcccagctt ctggttgcgc
    19801 agctctgctt tcgccgccga agacagtttg ctcatcggtt gaccgttaaa gatcacatcg
    19861 ccggaggttg gcgtatccag cccgcccaac aagtgcagca aggtgctttt accggaacca
    19921 gagctaccga cgatcgccat catttcacct tcgccgacgc tgaaactgac attgtgcagc
    19981 acatcggttt gcacactgcc ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg
    20041 atcttattca taacgtaaag cctcagcggg ttgagtggcg gcagcgcgcc atgaagggta
    20101 aagcgtagac agcagcgcga tagccatcgc caccagcgca ataacaatga cctgtaaagg
    20161 ttcgatagcg accggcagcg ccgcgccatc aagcaggacg ccgattatcg gcatcagatt
    20221 atttaactgg ctggcaagca gggcgccaag cgccgctccg aggatcgcac cgataatccc
    20281 ggcgctggcc ccttgcacca taaagaccat catgatttgt cgcggagtta agccttgcgt
    20341 ttgcaggatc gctacttcgc cctgcttctc cattaccatc agccctagtg aggtaataat
    20401 gttaaacgcc gcaacggcga caatcaggct cagcagtaaa cccatcatat ttttttccat
    20461 gcgtacggcc tggaacaact cgcctttacg atcacgccag tcctgccatt tgctgccttc
    20521 aggcagtttt tgctgactta atgagtcgac tttcagcggc tcatccagcc acaaacgcca
    20581 gccggtaata ttgcctgccg gataacgcat cagacgcgag gcatcctcaa tattcaccag
    20641 catttcattg ccatcgactt cactgttggc ggcgaaagtg ccaatcacat tgaacaggcg
    20701 ctgacttgga atacgcccca tcggcgtgaa ctggctggca gatggtacca tcatgcggat
    20761 ttgatcaccg cgattaacgc ctagctgtga ggcaagttgt tcgccgagga tgacattata
    20821 tttccccggc tcgaggtcag tttgtttcac attgaccaga tacggcgtta gtggatcttt
    20881 ttgcgccggg tcgataccga gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac
    20941 cacatcaccg gtagtaatag gtgcgacgcg attaacgccg tccagtttga ctgccgtttc
    21001 cgggagttgc tgcgggttaa gagagccatg ctcagaagag agaattgcct gtggcatcag
    21061 gccaaggatg ttgttttgca gctcgcgctc aaagccgttc atcactgaca atactgtgac
    21121 cagcgccatc accccgaggg taatgccgat ggtagaaagc caggagacga aacgaccgaa
    21181 gcgatccgct gcacgcccac gcatgtaacg caggccaata aatagagcga caggttggta
    21241 catgaaatcc gtctggttgc tgtagcaaag acacgagtat ataagcgaaa gcaagatggg
    21301 taaacgtctg aactgtaagt gatcgtcagg gctaatctga aaagcccccg taaatcaatc
    21361 ggctaaattg ccatgtttta tcatcaatct tgccgcctga tcttaatctg aaaaatcatc
    21421 caatacagac agatacccat tacacctcgg gtcataaccc gcaggatcaa ccgcatgtaa
    21481 attaaaaaag agcgtgttgt cctgatgaaa caaaaagagc tatggattaa ccagatcaaa
    21541 gggttatgta tttgtctggt ggtgatttat cactcggtca ttacctttta tccgcatctg
    21601 accacttttc agcatccgtt atcggaagtc ctgagcaaat gctggatcta tttcaatctt
    21661 taccttgccc cctttcgtat gccggttttt ttctttattt ccggctattt gattcgccgc
    21721 tatatcgaca gcgtaccgtg gggaaattgt ctcgataaac ggatctggaa catcttctgg
    21781 gtgctggcgc tttggggcgt ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg
    21841 gcacctgagc gcgatttaag caatgcctcc aatgccgctt atgccgattc caccggtgag
    21901 ttcctgcacg ggatgatcac cgccagcacc agcttgtggt atctgtatgc gttaattgtc
    21961 tatttcgtgg tatgtaaaat ttttagccgt ctggcgctgc cactattcgc cttgtttgta
    22021 ctgctgagtg tggcggttaa tttcgttccc acgccgtggt ggggaatgaa cagtgtgatc
    22081 cgcaatttgc tttattacag ccttggcgca tggtttggcg caacaataat gacctgtgtt
    22141 aaagaggtgc cgttgcgccg ccatctgctg atggcttctt tgcttaccgt tctggcggtc
    22201 ggtgcctggt tgtttactat ctcgctgctg ttgtcgctgg tatcgattgt ggtgatcatg
    22261 aagctgtttt atcaatacga gcaacgattc ggtatgcgct ccaccagcct gctgaatgtg
    22321 attggctcca acaccattgc tatctacacc acccatcgca ttctggttga aatattcagc
    22381 ttaactctgc ttgcgcaaat gaacgcagca cgctggtcgc cgcaagtcga actgacactc
    22441 ctgctggttt atccctttgt cagtttgttc atctgtactg ttgcgggctt gctggtaaga
    22501 aaactttcac agcgtgcatt cagcgatctg ttgttctccc cgccttcact gcccgctgcc
    22561 gtcagttact ctcgttaaac catcgcccca gccggggcga ttcaatttgc taaaccatgt
    22621 cgattacgga taatgaacag cattgcttat cagagatatg cccccatatg ttgaggcata
    22681 tcctaacgag aatctgacaa ccgttatgcc tgaacaatat cgttatacgc tgcccgtcaa
    22741 agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca gcctgtgcaa cgctggtagc
    22801 ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt gcaccagata tgcaaaatgc
    22861 tctgcgtttg catgatgaaa tcagccagtt caccgatcag atggtgatga atctggcgga
    22921 ctgggaaact cttccctacg acagtttttc gcctcatcag gacattatct cctcgcgcct
    22981 ttccaccctt taccagctac cgacgatgca gcgtggcgta ctgattgttc cggtgaatac
    23041 gcttatgcag cgcgtttgcc cacacagttt tctccacggt catgcgctgg tgatgaaaaa
    23101 aggtcagcgc ctgtcacgag atgcattacg aacccaactg gacagcgccg gttatcgcca
    23161 tgttgaccag gtgatggagc acggcgaata cgccacgcgc ggcgcgttgc tggatctctt
    23221 cccgatgggg agtgagctgc cttatcgtct tgatttcttt gatgatgaaa tcgacagcct
    23281 gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa gtagaagcga tcaatctgct
    23341 gcccgcgcac gaatttccga ccgataaagc ggcaattgaa ctgttccgca gccagtggcg
    23401 cgataccttc gaagtgaagc gcgatccgga acatatttac cagcaagtga gtaaaggcac
    23461 attacctgcc gggatcgagt actggcagcc attgttcttc agcgaaccac tgccgccgct
    23521 gttcagttat ttccctgcca ataccttgct cgtgaatact ggcgatctgg aaaccagtgc
    23581 cgaacgtttc caggctgaca cgctggcgcg ttttgagaat cgcggcgtcg atccgatgcg
    23641 cccgctgttg ccaccacaat cgctctggct gcgggtggac gagctcttct cagagctgaa
    23701 aaactggccc cgggtgcagc taaaaactga acatttaccg acaaaagccg cgaatgccaa
    23761 tttaggtttc cagaaactgc cagacctggc cgttcaggcg caacaaaaag cgccgctgga
    23821 tgcgctgcgt aagttcctcg agactttcga cggtccggtg gtgttctcgg tagaaagtga
    23881 aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt aaaattgctc cgcaacgcat
    23941 tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg atgattggcg ctgccgaaca
    24001 tggttttgtc gataaggtgc gtaatctggc gctgatctgc gaaagcgatc tgctcggtga
    24061 acgtgttgcc cgccgtcgtc aggattctcg ccgcaccatc aaccccgata cactgatccg
    24121 taaccttgcg gagctgcata ttggtcagcc ggtggtccat ctggagcacg gtgtcgggcg
    24181 ctatgccgga atgaccacgc tcgaagcggg cggcattact ggcgagtatt tgatgctcac
    24241 ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca ctgcatctga ttagccgtta
    24301 cgcaggtggc gcggaagaaa acgccccgct gcataaactt ggcggcgatg cgtggtcacg
    24361 cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg gcggaattgc tggatatcta
    24421 cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac gatcgtgagc agtatcagtt
    24481 gttctgcgac agcttcccgt ttgaaaccac gccggatcag gcgcaggcca ttaatgcggt
    24541 acttagcgac atgtgtcagc cgctggcaat ggatcgtctg gtgtgcggcg atgttggctt
    24601 tggtaaaaca gaagtggcga tgcgcgccgc tttcctggca gtagataacc acaagcaggt
    24661 ggcggtgttg gtgcctacca cccttctcgc gcagcagcat tacgacaact tccgcgaccg
    24721 tttcgccaac tggccggtac gtatcgaaat gatctcccgt ttccgcagcg ccaaagagca
    24781 gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat attctgatcg gtacgcacaa
    24841 actgctgcaa agtgacgtca agtttaaaga tttaggcctg ctgattgtcg atgaagaaca
    24901 ccgcttcggg gtgcgtcata aagagcgtat taaagcgatg cgcgcgaacg tggatattct
    24961 gacgcttact gcaacgccga tcccacgtac gctgaatatg gcaatgagcg gaatgcgtga
    25021 cctgtcgatt atcgccacgc cgcccgcccg tcgtctggca gttaaaacct ttgtccgtga
    25081 gtatgacagc ctggtggtcc gggaggcgat cctgcgtgaa attttgcgcg gaggacaggt
    25141 ttattatctc tacaatgatg tggaaaacat tcagaaagcc gccgaacggc tggcagaact
    25201 ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg cgcgagcgcg aactggaacg
    25261 ggtgatgaat gatttccatc atcaacgttt caacgtgctg gtttgtacaa ccattatcga
    25321 aaccgggatc gacatcccga cagccaacac cattatcatt gaacgcgcgg atcacttcgg
    25381 tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt tcacatcatc aggcatatgc
    25441 atggttgctg acgccgcatc caaaagcgat gactaccgat gcacaaaaac gtcttgaagc
    25501 gattgcctcg ctggaagatc tcggggcagg ttttgcgctg gcaacgcacg atctggagat
    25561 tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc tcaatggaaa ccatcggttt
    25621 ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca ctgaaagccg gacgcgagcc
    25681 gtcgctggaa gatctcacca gccagcaaac agaagtcgag ctgcggatgc cgtcgctatt
    25741 gccagatgat ttcattcctg acgtgaatac gcgtttgtcg ttctataaac gtattgccag
    25801 cgccaaaacg gaaaacgaac tggaagagat caaagtcgag cttatcgatc gcttcggcct
    25861 gctgccggat ccggcgcgta ccctgctgga tgttgcccgt ctgcgccagc aagcgcagaa
    25921 actggggatc aggaagctgg aaggtaatga gaaaggcggg gtgatcgaat ttgccgagaa
    25981 gaatcacgtt aatccggcct ggttgattgg tttgctgcaa aaacagccgc agcattaccg
    26041 ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg agtgagcgga aaacgcgtat
    26101 cgaatgggta cgccagttta tgcgtgaact ggaagagaac gcgatcgctt aatggtaaat
    26161 cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact tccctgcact
    26221 atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata accttatatt
    26281 tattatggat ttttggtgat gatcaaaacg cgtttttctc gctggctaac gttttttacg
    26341 ttcgccgctg ccgtggcgct ggcgctaccg gcaaaagcca acacctggcc gctgccgcca
    26401 gcgggcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga cggtggttct
    26461 ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca ggctaaccct
    26521 ggcgttgatc cttacgtacc gcgcgcgggt agcgtgttaa cgatcccgtt gcaaacccta
    26581 cttccagatg cgccgcgcga aggcattgtg atcaatattg cggagctgcg tctctattac
    26641 tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca gttaggtggt
    26701 gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa cccaacctgg
    26761 acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt gcctgcggta
    26821 gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc ggcctatggc
    26881 ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg ggtaagttct
    26941 ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt caccccaggc
    27001 accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa cggtgcgcgt
    27061 ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca gctgctgcca
    27121 attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga cgctgaagtg
    27181 atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg tcatcaagtg
    27241 agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc atgtcgcggg
    27301 gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa attactgctg
    27361 ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt gcgcccatct
    27421 catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc gcgttagcac
    27481 tgattgtacc gactttttgt tggccttctg gcgttgactg aacttcgaca gccgcaaagc
    27541 tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg tttttcataa
    27601 tagtggcctt atgcagatga atgacgtcgc aagcaattac ttaacgatcg ataactaaa
//
LOCUS       DAMZII010000058        27606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZII010000058 DAMZII010000000
VERSION     DAMZII010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.058"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..226
                     /locus_tag="POA77_002393"
     CDS             <1..226
                     /locus_tag="POA77_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6365822.1"
                     /translation="SVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIA
                     DIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            238..831
                     /gene="rclC"
                     /locus_tag="POA77_002394"
     CDS             238..831
                     /gene="rclC"
                     /locus_tag="POA77_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDI6365823.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(990..1118)
                     /locus_tag="POA77_002395"
                     /pseudo
     CDS             complement(990..1118)
                     /locus_tag="POA77_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            1421..2278
                     /locus_tag="POA77_002396"
     CDS             1421..2278
                     /locus_tag="POA77_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6365824.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKPTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(2399..6652)
                     /gene="fdeC"
                     /locus_tag="POA77_002397"
     CDS             complement(2399..6652)
                     /gene="fdeC"
                     /locus_tag="POA77_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDI6365825.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAANTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAAIYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            7768..7869
                     /locus_tag="POA77_002398"
     CDS             7768..7869
                     /locus_tag="POA77_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365826.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            8232..8495
                     /locus_tag="POA77_002399"
     CDS             8232..8495
                     /locus_tag="POA77_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6365827.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            8495..8635
                     /gene="ykgO"
                     /locus_tag="POA77_002400"
     CDS             8495..8635
                     /gene="ykgO"
                     /locus_tag="POA77_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDI6365828.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(8670..8897)
                     /locus_tag="POA77_002401"
     CDS             complement(8670..8897)
                     /locus_tag="POA77_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365829.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            9720..10262
                     /gene="ecpR"
                     /locus_tag="POA77_002402"
                     /gene_synonym="matA"
     CDS             9720..10262
                     /gene="ecpR"
                     /locus_tag="POA77_002402"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDI6365830.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            10337..10924
                     /gene="ecpA"
                     /locus_tag="POA77_002403"
                     /gene_synonym="matB"
     CDS             10337..10924
                     /gene="ecpA"
                     /locus_tag="POA77_002403"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDI6365831.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            10982..11650
                     /gene="ecpB"
                     /locus_tag="POA77_002404"
                     /gene_synonym="yagY"
     CDS             10982..11650
                     /gene="ecpB"
                     /locus_tag="POA77_002404"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDI6365832.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            11676..14201
                     /gene="ecpC"
                     /locus_tag="POA77_002405"
                     /gene_synonym="yagX"
     CDS             11676..14201
                     /gene="ecpC"
                     /locus_tag="POA77_002405"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDI6365833.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            14191..15834
                     /gene="ecpD"
                     /locus_tag="POA77_002406"
     CDS             14191..15834
                     /gene="ecpD"
                     /locus_tag="POA77_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDI6365834.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLLIKPRDFSISIISDEYT
                     QTPSREGYVGSGEPALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            15803..16513
                     /gene="ecpE"
                     /locus_tag="POA77_002407"
     CDS             15803..16513
                     /gene="ecpE"
                     /locus_tag="POA77_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDI6365835.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVRQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            16826..17155
                     /locus_tag="POA77_002408"
     CDS             16826..17155
                     /locus_tag="POA77_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI6365836.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(17403..18017)
                     /locus_tag="POA77_002409"
     CDS             complement(17403..18017)
                     /locus_tag="POA77_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI6365837.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            18435..19124
                     /gene="paoA"
                     /locus_tag="POA77_002410"
     CDS             18435..19124
                     /gene="paoA"
                     /locus_tag="POA77_002410"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDI6365838.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            19121..20077
                     /gene="paoB"
                     /locus_tag="POA77_002411"
     CDS             19121..20077
                     /gene="paoB"
                     /locus_tag="POA77_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDI6365839.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            20074..22272
                     /gene="paoC"
                     /locus_tag="POA77_002412"
     CDS             20074..22272
                     /gene="paoC"
                     /locus_tag="POA77_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDI6365840.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            22282..23238
                     /gene="paoD"
                     /locus_tag="POA77_002413"
     CDS             22282..23238
                     /gene="paoD"
                     /locus_tag="POA77_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDI6365841.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNWELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            23289..23627
                     /locus_tag="POA77_002414"
                     /pseudo
     CDS             23289..23627
                     /locus_tag="POA77_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(23754..23829)
                     /locus_tag="POA77_002415"
     tRNA            complement(23754..23829)
                     /locus_tag="POA77_002415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23794..23796),aa:Thr,seq:cgt)
     gene            24069..24983
                     /locus_tag="POA77_002416"
     CDS             24069..24983
                     /locus_tag="POA77_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365842.1"
                     /translation="MSSETSLRSQLELPNDQQIDVLAAILDKENEQATSARSMSTEPQ
                     EVLEKHPGITPFKDLVSKASEIVLTKNGNDIAQCLRSILENKNDYLNHRDEAVSLSIA
                     LNISQYIAPRFRALPPSPKRTSDDEPNQLSIDRQVIDMHWTWIQGQKKAVGAEEFEHL
                     FQREEFDWILAEKFARLNWTTDVKIRALQINVNLQWELAQYQSKKHRDSWRKLQKGDV
                     RGGKLHSLGIAQVTERLEVSVTKRRNLAKHIPHWVNVWLASRISDKSYMSLAQIMRFM
                     TGESHDEKRLKSSLKAVLQRLDSYEKKM"
     gene            complement(25490..25954)
                     /locus_tag="POA77_002417"
     CDS             complement(25490..25954)
                     /locus_tag="POA77_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6365843.1"
                     /translation="MKKSLRIQFGERVKELRVATGMSQEAFADRCGFARSYMSRIERG
                     GSNASLDAIEVLASALSVEPWQLLVSDSSEENDPELLVPYAADGTCFNPELASSRDGS
                     FAVGDKAAQKRFGSFIEALEYLRSMETAKWRRPNSAGNWGIVSAVKWDKLRK"
     gene            26193..26690
                     /locus_tag="POA77_002418"
     CDS             26193..26690
                     /locus_tag="POA77_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365844.1"
                     /translation="MAGSDFILPMIAGAVLSWASYSMVAHVTSKRQISTLRAISWGVL
                     GTLSLVLGLSAFAAVSAELPGEKQNALCLINEPECYAKAHHNPVKKCKQVMDEKATFR
                     HVWLDSEEQPVFSTYLWHDKSKQTIQIFGQQAKAINSLGIQTPLQYFCVFNANTGEVI
                     AASFE"
     gene            complement(26848..27405)
                     /locus_tag="POA77_002419"
     CDS             complement(26848..27405)
                     /locus_tag="POA77_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6365845.1"
                     /translation="MATFGYGRVSTTQQDTENQRLELEQAEWQIDYWFADIVSGKVPA
                     MQRKAFSEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRALAERNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTQAGLSRAKAEGKKLGRPSKIAPEARRAIIEK
                     KNSGTSISALAREYGVSRATIAALL"
BASE COUNT         7192 a         7018 c         6623 g         6773 t
ORIGIN      
        1 atctgtttta tttgcaacac tattatctgg cgttatggca ttttccacca atgcagatga
       61 taaaataatt ctgaaacata tcagcgtctc gtcagtatca gcatcaccga cagttctgga
      121 ggataccatt gctgatatag ccagaaaata taatgcttca tcctggaaag tcacatcgat
      181 gcgaattgat aataattcaa ccgcaacagc agtattgtat aaataaggat gttcacaatg
      241 gaaaaatacc tgcacctgtt aagtcgggga gataaaattg gcctgacatt gattcgtctg
      301 agtattgcaa ttgtttttat gtggattggg ttattaaagt ttgtccctta cgaggcagac
      361 agcattacac cattcgtcgc aaacagtcca ctaatgtcgt tcttttatga acacccggaa
      421 gactataaac agtatctgac tcacgaaggc gaatacaaac cagaagcaag ggcatggcaa
      481 tcggccaata atacctatgg tttttccaac ggtcttggcg tcgtggaggt gattattgct
      541 ctgctggttt tggctaatcc tgtcaatcgc tggttaggtt tattgggagg gctgatggca
      601 tttaccacac cgttggtaac actctcattt ttaatcacca ccccggaggc atgggtaccc
      661 gcattgggtg acgctcatca tggtttccct tatttatccg gtgctggtcg cctggtattg
      721 aaagatactc tgatgctggc aggtgcagtc atgataatgg cagattcggc gcgggaaatt
      781 cttaaacaac gcagtaatga atccagttca acgttaaaaa ctgaatattg ataatccgaa
      841 cactgtctgt tgttcacttt ttcaggcgtc aggccccctt tctgatgatg aagcctgacg
      901 ccatattacc ggagatatga cagagtgaag atcaatatca ctccctgtta aaaggttttt
      961 gtcgtcttag gtaaaataca tgattaatat caatcatgta tttttacagc aagacaacca
     1021 cgaacaaatt caggattaaa atgttttacc gactccccaa cataacccaa atcaagcgaa
     1081 ctaatttgtg ccatttcttt atctgtcagt gagaaatcga tttttgatgg gcatctgcaa
     1141 tactctgaag cagactatac ggaaaattcc actgtcagtc cctccattag gcatgaacaa
     1201 tgagtctacg ttaaaacgta acctcaaagt agtatgtgga ttttgatatc acttatgcaa
     1261 aaaattcatt aataatgtag gactgaaacc tctctatttt cggggacaac gaagcagacg
     1321 ctaccagtgc ttttgccttc gcccttgcta tttttgatac acttagggcc cagggtataa
     1381 cgaaaatgtg cgatatgaca aagaattaac ggagaatgag atgatcaggc agaagattct
     1441 acagcagctc ctggagtgga ttgagtgcaa tcttgagcac cctatttcaa tcgaagatat
     1501 cgcacagaaa tctggctaca gcagacgcaa catccagctt ctgttccgaa atttcatgca
     1561 tgtgcctttg ggagaataca ttcgcaaacg aaggctttgt cgtgccgcca ttcttgtccg
     1621 gctcaccgcg aaatctatgc ttgatattgc actctctttg cattttgatt cacagcagtc
     1681 attcagccgc gaatttaaaa agttattcgg ctgctctccc cgtgaatacc gccaccgtga
     1741 ttattgggat ctcgcaaata tcttcccttc ttttttaata cgtcaacagc aaaagacgga
     1801 gtgtagatta atcaactttc ctgagacacc tatttttggc aactcattta aatatgacat
     1861 tgaagtgtcg aataaatcac cggatgaaga agtcaaacta cgacgtcatc atttagccag
     1921 atgtatgaag aattttaaga cggatatcta tttcgtttcc acgtttgaac cgtcaacaaa
     1981 atcggtcgat ttgctcacgg ttgaaacttt tgctggtacg gtatgcaaac caaccgattc
     2041 agagatgccg aaagagtgga caattaaccg aggactgtat gcctctttcc gctatgaagg
     2101 tgagtgggag cactaccctg aatgggcgcg aaacctctat ctgatggaat tacccgccag
     2161 ggggttagcc agagtgaacg gcagtgatat tgagcggttt tattacaatg aagatttcgt
     2221 agaaaaggat agcaatgatg ttgtttgcga aatttttatc cccgttcgtc cggtttagtt
     2281 ttcgctatat ctaaatgaat ttataacgag actactgtca ggaaattcaa taaataatgg
     2341 cgttgaagat gtcaacgcca ttattgttta ttagaatgtt actttcatat tcttaaaatt
     2401 atttcatcgc ctcctcgtcc tttgccgtat cggcatccac cgcggtaatg gtcaccgtca
     2461 ctggcttcat ttcgtacccg ccataggtca gggcgttaaa tgtgaacgta tagacaccag
     2521 gcttgctggt actaaattta cgcgtgattt ttccgctcac gaaatggtca tttttcgacg
     2581 gcaggaactg ataatccttc tccgtcaccc cttccggcgt ctgaatgtcg acccacatat
     2641 tcccgccaac ggggttgttt gccgacatca gtgtcacctc cagcttcgca gtcgatttcc
     2701 catccgccac cagagattgc atatcagatg atgcggtgag taccgcgttt ttatcatccg
     2761 caacgaattt agattcggca gttttcgttg atacctgacc gttggtctga ctcacttgcg
     2821 ccttgagtgt gtatatcgcc gcaacagtag tggtagcggt gaaaatagcc tgcccttgct
     2881 cattcgttgt cgcagtgcca ttcggttcca ggtttaacga agccgggctg gcagtcagcg
     2941 tcacttcgct atctttcacc aaattgttat tggcatcagt caccgtaact ttatacgttt
     3001 gtttggcttt gccgtccgcc agcgcatagt tgcccatcac ctcaataccg gagacggttg
     3061 cggtagcctt atcggctaca aacgttatag tctgcgtatt cgaaggctgg ctattccccg
     3121 ccgatgccgt gatctcatac gttccggcag tcactgaaac cacctgcaac tctgctttcc
     3181 cctcatcgtt ggctttcacc cagacattat cgcctgtaag cggcttgaca ccccggggca
     3241 gattaaaggt caccaggctc cctggaacag ggttaccatt tttatcctca acggtcgccg
     3301 tcagcgtaaa ggcatctttg ccgtttgcta ctttttgaac ggcggtgtct acctgcaggt
     3361 ttgcctgacc ggtgctggca tccgcgttga atttcaccgt gaccgtcttc tgagagtttt
     3421 tcaccgcggc cgcgatttca agttcccctg caacggttga tatcaactca atatccgctt
     3481 tacctgccgc attggttgtt actgtattcc ccgtcttgct ggtcacaccc tgcggtaaat
     3541 tcagcgtaac ttcttgcccc tgcaacgggt taccatagct gtccacgacg gtcagggtta
     3601 tctggttagc agactgtcca ttagccagtt ggttattaac cttcactgtc atatcaccaa
     3661 tctcagcctt agatgcgtca cctgcaacat tcagcaccag tggctgagca acggcatttt
     3721 ggccgttcac tcgcggcatc acagacaact gacccgcggc agatcccagc gttaaggtcg
     3781 acacgtagac cccattacct ttctctgtcc agtttcctgc tgaaggacgc tcactccccg
     3841 tgttggcggc accgctaaac actggtgcat ctggcttcat cccggtgacc gggttcccgt
     3901 acgcatcctt cacggtgaag gtgagttccg tcgtcgtttt gacggtcgga gccttattgt
     3961 ccgcaacaag cgtagagttc gctgatgaca tctctccggc gatgaccgtc aactgcgcgg
     4021 cttcggtggc tgctggctgg ccgttgaata gcggcatgac acgaagctcg cccgtctttc
     4081 cgcctgtagt taacgtagca acataggaac cgtcaccttt ttcggtccaa ctggaaacgg
     4141 tcgccccttc agaggcggtc cccgtcaaac ttgccgacaa cgaaagacca ctgatagcgt
     4201 tgccatgcgc atctttcgcc accagcgtca cggttgtact ttcgccggct tttacgtgat
     4261 cttctgcgac agagactttc gactggtttg aagataacgc atcagccacc acggttactt
     4321 ttgccgcatt tgctgccgca tcctgtccat ttagcttcgg cataacttct aattcacccg
     4381 ccgtagtgcc gagagaaatc tgcgcagtcc aggtcccgtc gccattatcc gtccagcctg
     4441 atgccgtaga acctacagca gctgcgcccg ataatgacgg tgcatccgga ttgagaccag
     4501 ttacagggtt gtcattagca tcttttgccg tccagattgc cgtaactgta ccgccaacca
     4561 ccggtttatc aggattcagt gtgatggacg aatgtgctgc atcaagcggc ccggcaacaa
     4621 acttcagcgt ttgttgtaat gtgcccagct gatactgctc gctgaaagca cgcacaacaa
     4681 cgtttccggc acgggtcgaa gaaaccgtgg cgctgtaaac gcctggtttt atttccgaaa
     4741 tggcaccaac ggttacacca ttagtgtctt gcggaacaaa tcgcaagcgg ctggcttctc
     4801 ccgtcaccgg attaccctcg gagtccaccg cagtcaacgt caacgtatag gcttgctgac
     4861 catcagcaac cacgtcacct gacggctcgt tagcgctcag ggtggagttt gccacatcca
     4921 tcatcgtggc ccgcagttct gcagtgacgg ttttgctcat gccatcaaca ctaacagtaa
     4981 tcgttgcctc acctgactgc gttccagtag taaagacaga ctgatacacc cctgcttcag
     5041 tttcggtgaa ctcacccagt gttggctttg cctgtgattt agtggccttc agggtacgag
     5101 tcacaatatt tccagccggt ttgaaagtta gttcagtctt gatctgatct ttcatgcccg
     5161 tgactggctg gccctcggcg tcgcgcagag acagcaccag cggtctttgc tcattaccgt
     5221 tagcaagcat ttgaatacgg ctctgaccgt caagcgttaa cgccgtgcga tcggcgctca
     5281 taccagctcc ggtaatgacc acctctgtct gcacgcgttt tgaggtattg cctttgttat
     5341 cgtaggcaac cgcagaaatc gcataataat tgtctttgcc tggacgataa gccgggagcg
     5401 ttacttgcca ctgactaccc tgaccggtaa ttttgccacc ttcagccagt aatgacggcg
     5461 cttcccactg cacatttttc agtccgtgag ttgctttact gaccacaagc cccagggaaa
     5521 gtgtctgacc gcccttacct tcaatacgct caggcagagc aatacggatc acttcagatt
     5581 tgcggtactc aagaacgatg ttgttattac gctcaaccag gtcatagcgg ctgcctgcca
     5641 gtacccgacg ctcgcgaatg ctatccgtat cgagttgttt ctccagaggt tcgccaattc
     5701 ggtagttaac ttccaggcca aagcgagtgt cattctcacc gctcttgccc tgcttatgcc
     5761 cggcgctcag tgtcagaaga ggcactggcg tataggtcac ctcggcagaa atagcatgcg
     5821 ggtctttctg gcgcttatct ttaccaaaca gcccgacttc atcgccataa tactgttcat
     5881 acatcaggct tgcgccaagc tgcggccagg caggtaaata gccctctgcg cggatatccc
     5941 aaccattcgc cgggcgttcc tgataatcct caatatccgg cgattttttc cagcccgaag
     6001 cccgaatata accattggcg ctcagtttca gataatcgcg ccagtattcc gcaccaacac
     6061 caatgcgggt atgactacgg gataaatcat ggtcgataaa ggtgttcacc cccgccatcc
     6121 agtcatttcc tgaaaaatga cgccagccaa aaccaatatt tgactgagta cgatcgtcgg
     6181 tacgatgtat tgctccctga gtgaacaaca tatttgttgg cgtatcataa atcggataaa
     6241 gcatttccag cgaagaatcc ttcagcgaga aatctttatc gacattcagt ttgacgcgcg
     6301 cagtaccata tttcccgagc cactcctgta tttcctggtt agctttagct gtggccattc
     6361 cggtaataaa gttacgtgtc gcatcgctat ctggctgact gcttaaaaat gtcccggcat
     6421 ttgcggcaaa cgacgcgaca tttttctcca cgttattatc agcagttacc gtagtatttc
     6481 ccatgctcaa ccgtggctga accgcatgct gcgcacgtgc cgccattact ggggtaaagg
     6541 tgacagcgag tggaaaaaga acctgaacag agatatttgc ccacgccacg cagcgggcca
     6601 gaactgaata acgaaatcgt ggttgtttat gacctgtttt ataacgcgac attgagataa
     6661 tttcctgatg aacgaattaa cgaacatagg cagacacaaa taatggcgtc tgcttgtcag
     6721 aaaaaccggg gatttcccga tttccttttc cgcagaaaag gaaatacctt tatgtaagaa
     6781 agtgcaccgc cacctaatat atccagtgtc tgtagccact catcaggtgg ttgggcgcgg
     6841 gattatattt ttatttatgg gtgaaaattt agctttttgt gcaagtcatt aaatagacac
     6901 ctaagttata ttttttactc tgcggacagt gaataattca ttattcagag tgaataataa
     6961 tggcctgcct ttaattaaca actattggaa atacgtcgaa gtaggtatat acgccagaat
     7021 aaatcgatat attttttatg gatattcatt ttccttcatc ggactgtaaa ttgcggagat
     7081 ttcgaacttg cactacccat cgcaacactc aaacagattt atgaatttcg ttcataaccc
     7141 ctgacaaatc ctgccgtcta atcgagcttt tccctttacg ttatgcttct catgaacgag
     7201 aacacaactt attcatgaat taaccattcc ggataaacta tggaatcaaa ttcaggcatc
     7261 gaaaggaact ggctggtttc cgttaataga ttaaatatct attactctcc cgagagtaaa
     7321 actatcaaat caatgagtaa ccgatacagc ctcacaaatt ctttcagata attaacccgt
     7381 cactgcggca cgttctctca taaggcgtgc ctgcagtgta atccagtatg tatccggcat
     7441 agttgacggt ttaatagaac tcaattcctt tcccaggtta ccaacaacga tatcttgtat
     7501 aacaccccag gagatacgac attatgatga aatgaggtat gctcctcagc gccaatcaac
     7561 gcccggactg ggccagtctt gagatcagac tcattcccgg aactgacttc aggcgacata
     7621 tatcaatgac gttggtgcaa taaaagatag ccgatgtaat acatctattc aaatttgtga
     7681 tacatgtcaa aaaattggtt gaccaaactc gttatttata taagggcact tacgaagtgc
     7741 gctctttttt aaagcgagga agtaccaatg aaagagaata aagtacaaca aatcagtcat
     7801 aaactgatta atatcgttgt ttttgtcgca attgtagaat acgcctattt atttctccat
     7861 ttctattaat aacggcaata aactgttcac ttcagtgata tttaaaatat gcatcctctc
     7921 cctttttgta agtaattatt atatccgtgg gagaggaata cacgctgtca ggtaatcaat
     7981 catactgcga taaatcatcg gccagtaaag tggagataac ctccattgtc gaaaaatcca
     8041 tactctcagc aaaaccatca tcaatcactc atccaggcgt ttatgggagc gtcaccaatg
     8101 gctgctaaca atgccagagt tccccgttgc gaaaattcca catccacaaa gagtcacagg
     8161 gattgggtgt tgaaatgatc cggatgagca tgtatcttta tggttatgtt ataacataac
     8221 aggtaaaaat gatgaagccc aatatccatc ctgagtatcg tactgtggtg ttccacgaca
     8281 ccagtgttga tgagtacttt aaaatcggct cgactatcaa aacagaccgt gagattgagc
     8341 tggatggcgt aacgtatcca tacgtgacaa ttgatgtctc ttctaaatcg cacccgttct
     8401 atacagggaa gctgagaaca gtggcatcag aaggaaatgt tgcacgattc acccaacgtt
     8461 ttggtcgttt tgttagcacg aaaaaggggt cgtgatgaaa gttcttaact ctctgcgtac
     8521 cgcaaaagaa cgccatccag actgtcagat tgtgaagcga aaaggacggc tatatgtgat
     8581 ttgtaaatct aatccacgtt ttaaggccgt tcagggtcgt aagaaaaaac gttgattcaa
     8641 aattcgacgg attaacgata tttgtctgat taataatcag atcggattaa tgttggtgtg
     8701 tttataacac caacattaat tttcctgggg atatattcct cctgttcatt tgaggccaac
     8761 tgcctgacgt ttctctccga atattccatt atcttaatgt tgacttgttg accagcttcg
     8821 cccctgtatg ctggcatcaa ctctctttta gactgaacac gccactcagt ctcctccctt
     8881 tgcggcgcag cctgcatttt cactcaaact gttaagatga taaatgtggt aaatctgttg
     8941 gtactaacat aaaaacgttt acgccacagg aacagtctga tccaccggta accccgtcgc
     9001 cgacgttcga gtgccagtta gagtaacgca cacagataac tgcactcagt gccctgacaa
     9061 agaggccatc gttcctgtga cagctggcag ccttcgttta acttcactta atctggctct
     9121 tgggggctta ccgaacagat gacgtacata cgcccgttca attttccatc acttattgta
     9181 atgaacacct ataaccattt tgtgcggcat gttaatccat taaaatttct tactgattgg
     9241 caaatcatct tcaatgacag ctcattatag ttttatattc tatcccttac ccttaaaact
     9301 tgttttttta ctagtccatc acacagcgca ttaagactat tcctaacact tcagggcaaa
     9361 gttcctgacc aatataaaat gcaagtaaga attgaacgtt atattgccaa taaccttatg
     9421 aaaccaaatg tctttttctt cttatcaaaa aagcaatatt ttcagttttt ctaaatattg
     9481 acttaaccat tgaattcctt ttccgttcac atattgacac tcatcgggaa aaaaacataa
     9541 atttaagccc aatcgaaaat aattaaactt aatctcgttt aacctttatt gatatgtact
     9601 acgtatctta tttacttccg gtttactaag gaaactgaat gcacctgtaa aaattacagg
     9661 tttggaaagt agtgacatgg caaagtgatt acagcaggga ctatgaggtt aaaaaccata
     9721 tggaatgtca aaaccgttct gataaataca tctggtctcc ccatgacgcc tacttctata
     9781 aaggactatc tgaactgatt gtggatatcg acagattaat ttatctatcg ttggagaaaa
     9841 ttagaaaaga tttcgtgttt atcaatctca atacggattc tttatctgaa tttataaacc
     9901 gtgataatga atggttatcc gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa
     9961 agtcagaagc cttagcaaat tattggtatt acaacagcaa tattaggggc gtggtatacg
    10021 caggactgag tcgtgatatt agaaaagaac tggcctatgt gattaatggc aggttcctga
    10081 gaaaagatat taagaaagat aaaatcacgg accgggaaat ggaaattatc cgcatgacgg
    10141 cccagggaat gcaacctaaa tcgattgcca gaattgaaaa ttgtagtgtg aagacagtgt
    10201 atacccatcg gcgtaatgct gaggccaagc tgtactcaaa aatatataag ttggttcagt
    10261 aaactccagg caagttagtt ttaaaaaatg actcactggg acatcacgtc ctcaattcaa
    10321 ctcgggaaga aatgcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac
    10381 cggcatgggt gtggcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcggc
    10441 aacagccaaa aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt
    10501 ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa ggtctatttg acgtggctat
    10561 cgagggtgac tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacatt
    10621 aacccagttg gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc
    10681 agtcgaaaaa actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa
    10741 ccttagcccg ctggctaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt
    10801 caccttctcc atcatcagcg gtaccaccaa tggtaccacc gcagtaacag attacagcac
    10861 tctaccggaa ggcatctgga gcggcgacgt tagcgtacag ttcgacgcga cctggaccag
    10921 ttaatctctc tgatgtacca gcaggggtag cccccctgct ttattcctgg acggaattgt
    10981 tatgaaaaag caccttctgc ctctcgctct gctgttttcc ggaatatctc cggcccaggc
    11041 gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc tgagcaaaac
    11101 gatcaaaaac agtaccgaca gtggtcgcct tatcaatatc cgtctcgaac ggctctcttc
    11161 accgcttgac gacgggcagg ttatctcaat ggacaagccg gatgagttgc tactcactcc
    11221 cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc
    11281 cgcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc
    11341 gcagcgcgat aatgccaacc gcagcgctgt ggccactgct tccgcccgca tcggcaccat
    11401 tctggtcgtc gccccccgcc aggcgaacta ccactttcag tacgccaacg gctccctgac
    11461 aaatacagga aatgcgacgc tgcggatcct cgcctacggc ccttgcctga aagccgccaa
    11521 cggtaaggag tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg
    11581 cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc
    11641 cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag
    11701 gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg
    11761 ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg
    11821 cgcttcagga cggcatgagc gtcccgctct atattcatct cgccggtagc cagggtcgcc
    11881 aggacgatca gcgaatcggc agcgctttta tctggctgga tgatggacag ctacgcatcc
    11941 ggaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc
    12001 agctgatggc tctggccaac gccccgttca atgaggccct taccatcccc ctgactgaca
    12061 acgcgcagct ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag
    12121 cgctgggcac cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc
    12181 tcagcagtaa tctgagctat aacttgggcg tctataacaa ccagttgcgt aacggcggga
    12241 gcaacacatc cagctatctg tcgctgaata acgttactgc gctgcgcgaa caccatgtgg
    12301 tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa ttatataaag
    12361 cgatgtatga acgcgatttt gccggtcacc gatttgccgg tggaatgctc gacacctgga
    12421 acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct
    12481 ggggaaacca ggccagctcc accatcttcg acagcagcca gtcagccacg ccagtgatcg
    12541 cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc
    12601 agaacttcac tatgggcaat catgaagtgg atacccgggg tctaccgtac gggatttacg
    12661 atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag caaacgcccc cagcgggtca
    12721 ataagctgtt tagccggggg cgcggcgtcg gtgcaccact ggcgtggcag gtatggggcg
    12781 gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gctaaagaga
    12841 gttggctagc aggtgcctcg acctccggct cattgagtac gcttagctgg gcggcaacgg
    12901 gatatggata cgataatcag gcggtgggtg aaacccgtct gacgctgccg cttggggggg
    12961 cgatcaacgt taacctgcaa aatatgctgg ccagtgacag ctcatggagc agcatcggca
    13021 gcatcagcgc cactctaccg ggaggcttta gttcgctgtg ggttaaccag gaaaaaaccc
    13081 gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca
    13141 acctgaactc actgtggtcg aagctgggca cattcagcat cagctacaat gatgaccgcc
    13201 gttacaacag ccattattac acggcagatt actatcaaaa tgtctacagc ggtacctttg
    13261 gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc aacgccaata
    13321 cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga
    13381 tgacccatca aaacggctac accatggcaa acctgtcagc acgcaaacag tttgatgaag
    13441 gaaccattcg cactgttggt gccaatctgt cacgtgccat ctccggcgat accggtgatg
    13501 acaaaaccct cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc
    13561 tgaacgtcaa tagcgcggcg gacggctaca tcaataccaa cttgaccgcc aatggcagcg
    13621 tcggctggca gggtaaaaac atcgctgcca gcgggcggac tgatggcaac gctggggtga
    13681 tattcaacac cgggctggag gacgacggtc agatcagcgc caaaatcaac gggcggattt
    13741 tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg
    13801 agttacagaa cagcaaaaac tcactcgaca gctacgatat cgtcagcggc cgcaaaagcc
    13861 atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta
    13921 ccgtctccgg tcgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca
    13981 accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggac gtggataaga
    14041 aatatcccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gcactggaac
    14101 tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt
    14161 gggcggcggt gacgcagaca ggagaagaga atgagagtta acctactaat aacgatgata
    14221 attttcgcgc taatctggcc agtaactgag ctcagagcgg cagtgagcaa aacaacctgg
    14281 gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc
    14341 gtcacgcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta
    14401 aaatacaatg gttcaggaac catctatcag caaagcctcg gctacattga taacggttac
    14461 aacaccggcc tctataccaa ctggaagttt gatatgtggc tggaaaattc gccggtttca
    14521 tctcctttaa ctggcttacg ctgcatcaac tggtacgcag ggtgtaatat gaccaccagt
    14581 cttatcctgc cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc
    14641 ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt accagtatat gcaacaaatg
    14701 cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac
    14761 gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc
    14821 acccatacga aagcagcaaa tctgcggtta ataaataccc actctctggc ggaggtattt
    14881 atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg gacacagacc
    14941 atcggcagcc tttcaggatt aagttgtaag atggtcaact ataccctgca aacaaacgga
    15001 ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc
    15061 gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat
    15121 accgcctatt actacacctt caacgagatg aagagcgcag actcgatcta tgttttcttc
    15181 tcgagcaact tctttaagca gatggtgaac ctggggatca gcgacatcaa caccaaggat
    15241 ctgttcaact ttcgctttca gaacacgaca tcaccggagt ctggctggta tgaattttct
    15301 acctccaaca cgctgcttat caaaccccgt gatttcagca tcagtattat ctccgatgaa
    15361 tatactcaga caccgtcgcg ggagggatat gtcggcagcg gcgagccggc actcgatttc
    15421 ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc
    15481 ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag
    15541 gcgaaagtac cgttcccggc gacgctttcc tttattaccc gcaacggagc tacaaaaacc
    15601 tacgatgccg ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg gctaaccaca
    15661 ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg
    15721 attccaatgg ataacgccat ttcgctgcgt acggtagatg ataacggctg gtttggcgaa
    15781 gtcagcgctt caggagaaat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct
    15841 gctgacagcg gtctgtatgc tggcggcacc tttgacacag gcgatttcgg tcggcaatct
    15901 gacattttcg ctgccgtccg agactgactt tgtcagcaaa cgtgtagtga ataacaacaa
    15961 aagcgcacgg atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt
    16021 gcgcacccga ccggtggatg gtgaactgct tttcgccccc cgccagctgg cgttgcaggc
    16081 tggtgagagc gagtatttta aattttacta tcatgggcca cgggataacc gcgagcgcta
    16141 ctaccgggtc tcatttcgcg aggtccccac tcgtaaccag acaagacgca gcccaaccgg
    16201 cggcgaggtc agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg
    16261 tcaggttcag tttaaatggt ccttcgacaa ggtgacagga acagtaagta acactggcaa
    16321 cacatggttt aagctactga ttaagccagg atgtgattcg accgaagagg aaggcgatgc
    16381 ctggtatcta cgtccgggag acgtggttcg tcagcctgag ttacgtcagc cggggaatca
    16441 ttatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc
    16501 ttcggcggac taagacttat ccatcggtcg agggaagaat tccacctcag agctccaaaa
    16561 atcgtttaaa tgatgtggaa gcgatcgtca atgtcacctg taacgatggg ctttctcata
    16621 tgcacaccat agtgttcagg agacacggaa agatatcagg cagcctgcta acgagtggtt
    16681 taatcacgaa ttagtacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat
    16741 cgaaagttcc ccagccaacc gcggtacgtt cttacatacg atgtaccgct gttctcttta
    16801 cgatttatag ctgtactggt gaattatgag caatctgaat ccatgcatga cgtgtggtgc
    16861 ctgttgtgca tttttccgcg tctcttttta ctgggccgaa gccgacgatg ctggcggtac
    16921 cattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtaccaa
    16981 tcagaaaaac ccccgatgta ttgcccttgc aggaaccccg ggcaaaaatg cctgctgcac
    17041 gatatataaa aatcgatcgt ccacatgcag agaattcgcc atgtctggtg agaacggagt
    17101 cgtcaatgag gcttgcaatc gtgcaagggc taaatacggg ctgacaccgt tataaacata
    17161 caataattaa ttgcactgcc ccgccagcga taatagcggg gcttcgtttt tcaggggtaa
    17221 caaaacccga tacttcttct atttgccagc aacaatgcct ctcttctgta gcgttcttcg
    17281 gatctacatc atcctgaagt agcgcgatat actcactgac tatctgaatc agaatataaa
    17341 aagcaatgtt tttaacctat aaaaatggcg ctgtatttgc gccattttta tcattcaatg
    17401 cattatctgt ttgagcctaa agggatctca gggtctggct catgagtaat tctgtttcgt
    17461 aaatctctgc gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagaa
    17521 acattctcat accacgggag atcaaacaga ggtggcgtca gtcccatgag agggaatgaa
    17581 atcatatgaa caaaaagttg ggctaaagca cctgccagta agccctgcca gagtttaatt
    17641 tttggaaata cttcagcgac cacacaataa ccgacagcaa acactatcga aaagataatg
    17701 tgcgtaacac caacccagtt aaagacatgc ccggcaaagg tataaacagc cgcattggga
    17761 tctgtcagcc ccaaccagtc tcgaagaaaa atatacggtg gattgagaaa attacgcgag
    17821 caatcaattt ggcctgcagc cctgattaat gattccgggc cacacgctgc attaaacata
    17881 tccaccgggc tacgtggcgg caatggaact tcagcccccc acttcacgaa tgcggaaaca
    17941 acgccagcaa tcagcccaat gaatgcagca agaccataac gtctgcggtt cggtggagtt
    18001 tgttcaaata tattcatatc taccctgctt gtaccattat gttatacacc tcttcaggag
    18061 tattcataaa acaaggcaaa tgtaaagaac tgtattgttt tgtataacaa gatggtttcc
    18121 taatcgccaa tgaatataag ctccatcatt tctccctatt tttatattag aagtgacaga
    18181 gatttgcagg gtgatgcaga gctgaaaatc acaggtttcc ttattggttt ttgcatcgta
    18241 caactaaagc aataaaccag ctcccatcat catcaacatc ccggcgaaca tgaatttatt
    18301 agccgggctc atagctgcat tcgcgtttta agaataatcc tcctgctgtc gccgactatg
    18361 cttaacattt aaaaaagcat cagcactctc gcaacgcact cttattttcc cctttagaat
    18421 accggaggcc tggtatgagc aaccaaggcg aataccccga agataatcgg gttgggaagc
    18481 acgagccgca cgatttaagt ttgacccgtc gcgatctgat taaagtgagc gccgcaacag
    18541 cggcgaccgc cgtggtttat cctcattcta cgctggcggc aagcgttccg gcagctacac
    18601 ccgcgccaga gataatgccc ctgacactga aggtgaacgg caaaaccgag cagcttgagg
    18661 tggatacccg aaccacgcta ctggacgctt tgcgtgaaaa tctgcatttg atcggtacca
    18721 agaaaggttg cgatcacgga cagtgcggag cctgtaccgt gctggtcaat ggtcgcaggc
    18781 ttaatgcctg cctgacgctt gcagtcatgc atcagggggc cgagatcacc accattgaag
    18841 gcctgggttc gccagataat cttcacccca tgcaggcggc ctttatcaag catgatggct
    18901 tccagtgcgg ctactgcacc tccgggcaaa tttgctcatc agtagcggtg ctaaaagaga
    18961 ttcaggacgg cattcccagt cacgtcacgg tcgatttggt ttccgctcca gaaacaactg
    19021 ccgatgagat ccgtgaacgt atgagcggca acatctgtcg ctgtggtgca tacgctaaca
    19081 tccttgccgc cattgaagat gctgcggggg agataaaatc atgaaggcgt ttacctatga
    19141 acgagtgaac accccagccg aggcggcact tagcgctcag cgcgtacccg gcgcaaaatt
    19201 tatcgcgggc gggaccaatc tgctggacct gatgaagctg gaaattgaaa cgcccaccca
    19261 ccttatcgat gtgaacggcc tcgggctcga taagattgaa gtgaccgacg cgggtgggct
    19321 gcgcatcggc gcactggtac ggaacaccga cctggcggct cacgagcgcg tgcgtcgtga
    19381 ttacgcggta ctctcccgcg ccctgctcgc tggcgcatct ggtcagttac gtaatcaggc
    19441 aaccaccgca ggtaatctgc tccagcgcac gcgctgcccc tatttttacg acaccaatca
    19501 gccctgcaat aagcgcctgc ccgggagcgg ctgcgcggcg cttgaaggct ttagccgtca
    19561 gcacgcggtg gtaggcgtaa gtgaagcctg catagccacc catccgagcg atatggcggt
    19621 cgcaatgcgg ttgctggatg cggtggtgga aaccatcacg ccggagggaa agactcgcag
    19681 tatcacactg gctgattttt atcacccacc ggggaaaacg ccgcacattg aaaccgccct
    19741 gcttcccggt gagcttatcg ttgcggtgac gttacctcca ccgctcggcg gaaaacatat
    19801 ctaccgtaag gtgcgcgatc gcgcctccta cgcctttgcc ctggtatcgg tcgcggcgat
    19861 tattcagcct gacggcagcg ggcgcgtcgc gctgggcgga gtagcacata agccctggcg
    19921 cattgaggct gcggatgctc agctatccca gggggcgcag gccgtatatg acacgctgtt
    19981 cgccagcgcc catcccaccg ctgaaaacac ctttaaactc ctgttggcga agcgaacgct
    20041 tgcctccgta ctggctgaag cgagggcaca ggcatgaaat ttgataaacc cgcaggggaa
    20101 aacccgatcg atcagctgaa ggttgtcggt cgtccccatg accgcatcga cggaccgctg
    20161 aaaactaccg gcacggcacg ctacgcctac gaatggcatg aagaggcccc caacgccgcc
    20221 tatggctata tcgtcggttc cgccattgcc aaaggacgcc tcaccgccct tgatacggac
    20281 gccgcgcaaa aagcgccggg cgtactggct gtcattaccg ccagtaacgc cggggcactc
    20341 ggcaaaggcg acaaaaacac cgccaggctg ttaggcggcc ccactattga gcactatcat
    20401 caggccattg cgctggtagt ggccgagacc ttcgaacagg cgcgagcggc ggcctcgctg
    20461 gtgcaggcac actatcgccg taataaagga gcttactccc tggcggacga aaaacaggcc
    20521 gtcaatcagc cgccggaaga cacgcccgac aaaaacgtcg gtgactttga cggggctttc
    20581 acctccgctg cggtgaagat tgatgctacc tacacgaccc cggaccagag ccatatggcg
    20641 atggagccgc atgcctcgat ggccgtctgg gatggaaata agcttaccct ctggacctca
    20701 aatcagatga ttgactggtg ccgcaccgat ctggcaaaaa cgctgaaagt tcccgtggag
    20761 aatgtgcgta ttatctcccc gtatatcggc ggagggtttg gcggcaagct gttcctgaga
    20821 agcgatgcgc tgctggcggc cctcgccgcc cgagcggtga aacgtccggt taaagtgatg
    20881 ctcccccgcc cctctattcc caataacacc acgcaccgcc ccgccaccct tcagcacttg
    20941 cgtatcggtg ccgaccagag cgggaaaatc accgctatct cacatgaaag ctggtctgga
    21001 aacctgcccg gcggcacgcc ggaaacggcg gtacagcaaa gcgaattact ctacgccggg
    21061 gcgaatcgtc ataccggcct gcggctcgcc acgcttgatt tgccggaagg gaacgccatg
    21121 cgtgcgcccg gcgaagcccc cggtctgatg gcgctcgaaa tcgcgatcga cgaactggcg
    21181 gaaaaagcgg gcatcgatcc cgtcgagttt cgcatcctga atgacactca ggttgacccc
    21241 gccgacccga cgcgctgctt ctctcgccgt cagcttatcg agtgcttgcg caccggagcg
    21301 gataaatttg gctggaagca gcgcaacgcc acacccggac aggtgcgcga cggggagtgg
    21361 ctagtcggcc acggtgttgc ggcgggcttt cgcaataatc tgctggaaaa atcgggggct
    21421 cgggttcacc tcgaacaaaa cggcaccgtt accgtagaaa cggacatgac cgacattggc
    21481 accggcagct acaccattct ggcccagacg gcagcggaaa tgcttggcgt accgctggag
    21541 caggttgcgg ttcacctcgg cgattccagt ttcccggttt ctgcgggttc tggtggacaa
    21601 tggggcgcga atacctccac ctccggcgtt tacgccgcct gtatgaagct tcgcgaaatg
    21661 attgcctcgg cagtcgggtt tgatcctgag cagtcgcagt ttgccgacgg caagattacc
    21721 aacggtaccc gaagcgccac gctacatgaa gccaccgcag gcggcagact gacagcggaa
    21781 gagagcattg aattcggaac actgagcaaa gagtaccagc agtcgacctt tgccgggcat
    21841 tttgtggagg tcggcgtgca tagcgcgacg ggagaagttc gggtccggcg tatgctcgct
    21901 gtgtgtgctg caggacgcat cctgaatccg aaaactgcgc gcagccaggt cattggcgca
    21961 atgactatgg gcatgggtgc ggcactgatg gaggagctgg cggtggatga ccgtttgggc
    22021 tacttcgtta atcacgatat ggcggggtat gaggtgccgg tccatgcgga tatcccaaaa
    22081 caggaggtga ttttcctgga tgataccgac cccatatcct ccccgatgaa ggccaaaggt
    22141 gtcggtgagt tgggcctgtg cggcgtgagc gcggctatcg ccaacgcggt gtataacgcc
    22201 accggtattc gggtacggga ttatcccatc actctggata agctgctcga taagctgccg
    22261 gatgtggttt aaggagaaac aatgtcatac ccgctttttg ataaagacga acactggcat
    22321 aagccagagc aggcgtttct caccgatgac caccggacca ttctgcgctt cgccgtagag
    22381 gcgctaatgt ccggtaaagg agcggtgctg gtgacgctgg tggagataca cggcggcgcg
    22441 gcgcgcccgc tcggggcgca gatggtggtt cgcgaagatg gtcgttactg cggttttgtc
    22501 tctggcggct gcgtggaggc cgctgccgct tttgaagcgc tggagatgat gggctcaggc
    22561 cgcgatcgcg aaattcgcta tggcgaaggt tcgccgtggt ttgacatcgt tctgccctgc
    22621 ggcggtggga tcacgctgac gctccataaa ctacgctcgg cacagcctct gctcgccgtg
    22681 ctgaaccgtc tggaacagag aaaaccggcg gggctgcgct acgatccgca agcacaatcg
    22741 ttggtgtgcc tgcccacgca aacccgaacg ggctggaatc tcaatggctt tgaggtgggg
    22801 ttcaggccat gcgtcaggct gatgatttac ggacgttctc ttgaggcgca ggcaaccgcg
    22861 agtcttgcag cagccacagg ctatgacagc catatcttcg atctttttcc ggcctcagcc
    22921 agcgctcaga tcgataccga cacggcggtc attttgctgt gccatgatct caactgggag
    22981 ctgccagcat tgcaggccgc gcgagaagca aaaccctttt atctcggcgc attgggcagc
    23041 catcgaaccc acacgttacg tctgcaaaag ctccacgagc tgggatggtc cagggaggag
    23101 acagcgcaaa tccgggcccc cgtcgggata ttccccaaag cccgggatgc gcatactctg
    23161 gcactctccg tgctggcaga aatcgcctct gtacgtctcc atcatgagga ggattcatgc
    23221 ctgcccccgt cgtcctgatc cttgcggccg ggcgtggaga gcgctttctc gcctccgggg
    23281 gaaataccca taagtgtatc ggctggcgtc agtccccgga ggttgcgcct tatcgctggc
    23341 catttgaaga aaacgggaga actttcgacc ttgcgattga accgcagatt acgactaatg
    23401 atctgcgtct gatgttgagg ctggctcttg ccggcggagg aataacaatt gccactcagg
    23461 aaactttcag gccatatatt gaaagcggta agcttgtatc gctgcttgat gactttcttc
    23521 cacaatttcc gggcttctat ctgtatttcc cacagcgtcg caatattgca ccaaagctcc
    23581 gcgccctgat tgactacgtc aaagaatggc ggcagcaatt ggtttaaata gctgcacctg
    23641 cactgcctga tgtcagaaca gtattttgat gaattgccaa ggttacaatg gcacaaatgc
    23701 ggcacaggag gaaacgtggt gtatttaaat atggggtaac tctttgattt taatggtgcc
    23761 gataatagga gtcgaaccta cgaccttcgc attacgaatg cgctgctcta ccaactgagc
    23821 tatatcggct ttggggcgcg taaccataaa gcattaatca atgatgaaat cgcattgtag
    23881 gacagttttg taggagagcc aatgtcctct acagagcact aaaaaaactc atgacgtaaa
    23941 atagcctaaa ataaaaataa ttataaaaca atatgttatg ttaccgttta aaaatagcca
    24001 accagaagca ttgaaaagcc taagttcaca aaataatgtc ccgcttattc aactaaggag
    24061 tgggaattat gtcatcagaa actagtttac gtagtcagct tgagcttcca aatgatcagc
    24121 aaattgatgt ccttgctgcg attctcgaca aagagaacga acaggcgaca tcagcccgta
    24181 gtatgtctac cgaacctcaa gaagttcttg agaaacaccc cggtattact ccttttaaag
    24241 atctcgtttc aaaagcatca gagatcgttt tgacaaaaaa tggtaatgat atagcgcaat
    24301 gtctacgctc tatccttgaa aacaaaaatg attacctaaa ccatcgagat gaagcagtgt
    24361 cattgtcaat tgcgttgaat atttcacaat acatagctcc gagatttcgt gcgcttcctc
    24421 catcacctaa aagaacgtct gatgatgaac caaaccaatt aagcattgac cgccaagtca
    24481 tcgatatgca ctggacatgg atacaaggac aaaaaaaagc ggtaggtgca gaagagtttg
    24541 agcacctatt tcagcgtgaa gaatttgact ggatcttggc agaaaaattt gcgcgtctaa
    24601 attggacaac tgacgtaaaa atcagggcat tacagatcaa tgtaaacctt caatgggagt
    24661 tagcacagta tcaatctaaa aaacaccgtg attcatggcg gaaacttcaa aaaggagatg
    24721 tccgaggtgg caagttgcat tcattgggga ttgcacaagt cacggagaga cttgaagttt
    24781 cagtaacaaa acggagaaat ctagcaaaac atattcctca ttgggttaat gtttggcttg
    24841 cttcccgcat atccgataaa tcatacatga gtttagcgca gataatgaga tttatgacag
    24901 gggaaagcca cgatgagaag aggcttaaat catcattaaa agctgtttta caacgtttag
    24961 attcatatga gaaaaagatg tgaactcaaa atttgaatga aacgatagcc ttgaaaaaca
    25021 atttaccggg tttatgcgcg attagtttac ccagtatggg agctatctct tttgccataa
    25081 aacccgcgta aatcatagtt ctttgtatga ctagggtatt gcttagcttt ctaacttttg
    25141 taactaatta cccaataaca gggtcgcgac tttgtcgcga cgggctgctt acgcagcctg
    25201 agttatcata gaagaacaac tctcgggaag ggtgaagatt cggagcaaaa taaaactggc
    25261 aaaaactcac ggagtgagtt tattttttga atccaccaca aaaatcataa caacttgaat
    25321 ctaataataa aaaacattaa aagcgatacg taacgcctct gtaacgcacc cgtgtttcat
    25381 gattatctat atacagccga aagaatcaaa agtcctaggg tggacagcat ccattatggg
    25441 gatgcaatgt ataaatccta tttgccaaat ttatatcctt atactggcac tacttcctaa
    25501 gcttatccca tttaacagca gacacaattc cccaattgcc cgcagaattc ggcctacgcc
    25561 actttgccgt ctccatgcta cggagatatt caagggcttc gataaacgag ccaaaacgct
    25621 tctgagcggc tttatctcct actgcaaatg aaccatcgcg actactcgcc agttcgggat
    25681 taaagcacgt cccatccgcc gcatagggaa ccagtagttc tggatcgttt tcttcggatg
    25741 agtcggatac taacaactgc cacggttcaa cactgagagc gctagccagc acctcaatgg
    25801 catcgagaga tgcattagaa ccgccacgtt caatgcggct catatagcta cgtgcaaacc
    25861 cacaccgatc cgcaaacgct tcctggctca ttccggtggc aacgcgcaac tcttttaccc
    25921 gctcgccaaa ctgtattctt agggattttt tcatgcactg aatgtgcaat tttgaccact
    25981 gtaaggccac gctccatatg agacatttcg aatctaacga tccctattgt gagcacaatc
    26041 accctcacga gaagtctgta tcgctacaat caaagattca aaatgaatac tattagtgac
    26101 aaaacaagcc aatatcgtat gatggctgtc acaggttggg tgtcttcagc gctcactatg
    26161 agatgtgatg ttgtttggca aatgaggaaa tcatggcggg atcagatttt atattaccta
    26221 tgatagcggg agccgtgctc agttgggcca gttacagcat ggtcgcgcac gttacatcaa
    26281 aacggcagat cagcactcta cgagcaatct catggggtgt tctgggaacg ctctcactag
    26341 tgctgggatt aagtgcattc gcagctgtta gcgctgaact cccgggagaa aaacaaaatg
    26401 ccttgtgctt gattaacgag ccagagtgct atgcaaaagc acatcataac cccgtcaaaa
    26461 agtgcaaaca ggtaatggat gagaaagcga catttcgcca tgtgtggcta gatagcgaag
    26521 agcaaccagt tttcagcacc tacctctggc acgacaagag caaacaaact attcaaatat
    26581 tcgggcaaca agctaaggcg attaatagcc ttgggattca aacaccactc caatattttt
    26641 gtgtattcaa cgccaacacc ggggaagtca tcgcggcttc atttgagtaa ccactataag
    26701 ccccccgaac ttataagtgg gggcattcaa cctcgtcgat cttctttgct gctctaaccg
    26761 gaaacggtgt cacaatcttt ctttcgtttt gaatctctca cgctaaccaa cccaaaactc
    26821 ggggttgcaa tgaaagttta gaagcattta aagcagcgcg gctatagtcg ctctggaaac
    26881 accgtattca cgcgctaacg cactgatact ggtcccgcta ttctttttct ctataatcgc
    26941 cctacgggct tctggtgcga ttttcgacgg tctgcccagc ttctttcctt cagcttttgc
    27001 tcgtgacagc cccgcctgag tgcgttcaat aagtaggtca cgctccattt ccgcaacggc
    27061 tgccagcata gacaacagaa gcttaccggc agcagaagtg aggtcagtag ttccaagctg
    27121 gtggacgatc accttgatat tacgctctgc cagtgccctg accgtctgga gaacatcaat
    27181 cgcatcacgt cccagacggt ctagttttgc aactaccaac gtttcaccat cgcgaatttt
    27241 gctaagcatt tcagaaaacg ctttacgttg catagccgga accttcccac tgacaatatc
    27301 ggcaaaccaa tagtcaattt gccactcagc ttgctcaagc tccagtcgct ggttctcagt
    27361 atcctgctgc gttgttgaaa cacgcccgta gccaaaagta gccattgtta tctcctcatc
    27421 tgacccctgc cagaaaacga ggccaacatt aggatggcct gccagaaaag ccaaggctat
    27481 ttttccaaca gtgtgaaggc gtgcgattgg gaggtgccag aaaacggtcg atttcttaca
    27541 tcatggtttc tattgactac acaatcaaag tggttatgtt ctaatcgaaa cgtaaacatt
    27601 tcttta
//
LOCUS       DAMZII010000059        27455 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZII010000059 DAMZII010000000
VERSION     DAMZII010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.059"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(102..860)
                     /gene="chbG"
                     /locus_tag="POA77_002420"
     CDS             complement(102..860)
                     /gene="chbG"
                     /locus_tag="POA77_002420"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDI6365846.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVMDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(873..2225)
                     /gene="celF"
                     /locus_tag="POA77_002421"
                     /gene_synonym="chbF"
     CDS             complement(873..2225)
                     /gene="celF"
                     /locus_tag="POA77_002421"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6365847.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2330..3172)
                     /gene="chbR"
                     /locus_tag="POA77_002422"
     CDS             complement(2330..3172)
                     /gene="chbR"
                     /locus_tag="POA77_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDI6365848.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3180..3530)
                     /gene="chbA"
                     /locus_tag="POA77_002423"
     CDS             complement(3180..3530)
                     /gene="chbA"
                     /locus_tag="POA77_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDI6365849.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3581..4939)
                     /gene="chbC"
                     /locus_tag="POA77_002424"
     CDS             complement(3581..4939)
                     /gene="chbC"
                     /locus_tag="POA77_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDI6365850.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(5024..5344)
                     /gene="chbB"
                     /locus_tag="POA77_002425"
     CDS             complement(5024..5344)
                     /gene="chbB"
                     /locus_tag="POA77_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDI6365851.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(5643..5981)
                     /gene="osmE"
                     /locus_tag="POA77_002426"
     CDS             complement(5643..5981)
                     /gene="osmE"
                     /locus_tag="POA77_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDI6365852.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            6183..7010
                     /gene="nadE"
                     /locus_tag="POA77_002427"
     CDS             6183..7010
                     /gene="nadE"
                     /locus_tag="POA77_002427"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI6365853.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            7240..8127
                     /gene="cho"
                     /locus_tag="POA77_002428"
     CDS             7240..8127
                     /gene="cho"
                     /locus_tag="POA77_002428"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDI6365854.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(8087..8662)
                     /gene="ves"
                     /locus_tag="POA77_002429"
     CDS             complement(8087..8662)
                     /gene="ves"
                     /locus_tag="POA77_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDI6365855.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(8865..9350)
                     /gene="spy"
                     /locus_tag="POA77_002430"
     CDS             complement(8865..9350)
                     /gene="spy"
                     /locus_tag="POA77_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDI6365856.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(9680..10648)
                     /gene="astE"
                     /locus_tag="POA77_002431"
     CDS             complement(9680..10648)
                     /gene="astE"
                     /locus_tag="POA77_002431"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDI6365857.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGALSHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(10641..11984)
                     /gene="astB"
                     /locus_tag="POA77_002432"
     CDS             complement(10641..11984)
                     /gene="astB"
                     /locus_tag="POA77_002432"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDI6365858.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     FHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(11981..13459)
                     /gene="astD"
                     /locus_tag="POA77_002433"
     CDS             complement(11981..13459)
                     /gene="astD"
                     /locus_tag="POA77_002433"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDI6365859.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGDVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(13456..14490)
                     /gene="astA"
                     /locus_tag="POA77_002434"
     CDS             complement(13456..14490)
                     /gene="astA"
                     /locus_tag="POA77_002434"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDI6365860.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(14487..15707)
                     /gene="astC"
                     /locus_tag="POA77_002435"
     CDS             complement(14487..15707)
                     /gene="astC"
                     /locus_tag="POA77_002435"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDI6365861.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            16153..16959
                     /gene="xthA"
                     /locus_tag="POA77_002436"
     CDS             16153..16959
                     /gene="xthA"
                     /locus_tag="POA77_002436"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDI6365862.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            17126..17836
                     /locus_tag="POA77_002437"
     CDS             17126..17836
                     /locus_tag="POA77_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6365863.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            17841..18518
                     /gene="ydjY"
                     /locus_tag="POA77_002438"
     CDS             17841..18518
                     /gene="ydjY"
                     /locus_tag="POA77_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDI6365864.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            18533..19240
                     /locus_tag="POA77_002439"
     CDS             18533..19240
                     /locus_tag="POA77_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6365865.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            19240..19788
                     /locus_tag="POA77_002440"
     CDS             19240..19788
                     /locus_tag="POA77_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6365866.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            19798..20964
                     /locus_tag="POA77_002441"
     CDS             19798..20964
                     /locus_tag="POA77_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6365867.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQMGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            20937..22472
                     /locus_tag="POA77_002442"
     CDS             20937..22472
                     /locus_tag="POA77_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6365868.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            22472..23125
                     /locus_tag="POA77_002443"
     CDS             22472..23125
                     /locus_tag="POA77_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6365869.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            23192..24499
                     /gene="ynjE"
                     /locus_tag="POA77_002444"
     CDS             23192..24499
                     /gene="ynjE"
                     /locus_tag="POA77_002444"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDI6365870.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(24508..25128)
                     /locus_tag="POA77_002445"
     CDS             complement(24508..25128)
                     /locus_tag="POA77_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI6365871.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            25215..25622
                     /gene="nudG"
                     /locus_tag="POA77_002446"
     CDS             25215..25622
                     /gene="nudG"
                     /locus_tag="POA77_002446"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDI6365872.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(25588..25860)
                     /locus_tag="POA77_002447"
     CDS             complement(25588..25860)
                     /locus_tag="POA77_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDI6365873.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            26096..27439
                     /gene="gdhA"
                     /locus_tag="POA77_002448"
     CDS             26096..27439
                     /gene="gdhA"
                     /locus_tag="POA77_002448"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDI6365874.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6710 a         7271 c         6822 g         6652 t
ORIGIN      
        1 ttttgcgttt gtcagcagtc tcaagcggcg cagttacgcc gcctttgtag gaattaatcg
       61 ccggatgcaa ggttcacgcc gcatctggca aacatcctca cttacacatc ccgataactc
      121 cccaaccgat aaccacgctg agcgatagca cctttcaacg acgctgatgt caacacatcc
      181 agctccgtta agcgtgggaa acagtaagca ctctgacgga tagtattatc gataaacgcc
      241 ggatgacaca tcacctccag cgaacgatca ccccgatggc ttgcgtcatc catcacttgc
      301 aggaacagtg cctcgctaat ctcttcacca taaaacgcgc tactgaatcc ctgagaactg
      361 cgcagattag ccggtaaatc accagcgtta gatagcggct gacggtcgat gcgcaacgca
      421 atcccctctt cagccgcaaa cctggcaaca atcgggaaaa tctgcgggaa catatgcaca
      481 tgatgatggc tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac
      541 tgactggcaa gctcctgagt aatttcttcc agcggtaaag catcttcttc tgccaactgc
      601 cagatccatt ttcccagcac accatcgcgg gttaaccccg gcatagctgt cagtggcttg
      661 cccatagtaa ggacaaagtg catccctatg gccagactcg gttcatcacg actcaactgc
      721 accgcatggt caatagcctg cccattcacc agcgccgtcg ttgatgtgac aatcccattg
      781 cgacaggcct cgataatgcc gtagttctga cctttgctta agccaaaatc atcggcatta
      841 acaatcagta agcgttccat aatcagcctc ggttaatgtg cttttttaag ctctgcgatg
      901 cagtcggcaa aatttggcag ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc
      961 tcagcatcgc gatcggaatg caccaacgga ctaaggttta gcgccagtaa cacatcgtta
     1021 aattctccgc taagtgcggc gttgctggca gcaatctcga agcctttaat ggtgtgaatc
     1081 agccccatca ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc
     1141 cccagcttac aggtcatttc taccgcccag tctgccggaa tattatcaat atgcccatga
     1201 tgcgggatat taacgtaatg ttcagcttgc ttgtcgttgt agatagcgtt gatcacttcg
     1261 cacgctgcat cagagtaata agccccaccg cgctgttcca gttctttcgg cttaactttc
     1321 aactccggat ttttatacag ctcaaaaagt tgtttctcga ctttctgtac tacctgtgct
     1381 cgtgcgccgc ctttgtagta ttcgcccatt tcaatagcca gcatctcttt ctgcttgaag
     1441 taatacagca gataagaaca tggcagcaga tttaacgaac gaattaagcc ctcactaaat
     1501 ggcagatcga aaatattttt tacagaggac gcttttaact gccctgacgc cacaccatca
     1561 agcaattcgg caaagcgcga cttgccattt accagcacat ccttaatgaa caccatatgg
     1621 ttgaggccga acagatcgat agataaatca tcgctgtctt tcagcatcag aacatcgcga
     1681 ataaacatct tcatgccgat cggaatatta cacacgccga taaagcgttt aaatccggta
     1741 tgacgataaa cggcttcagt gaccattccc gccgggttag tgaagttaat cacccatgca
     1801 ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga
     1861 cctttaaaca gaccgcccgc gccgttggtt tcctgaccaa gataaccatg acttaatgga
     1921 atacgttcat ccagttcgcg cgccggtaat tggcctacgc gcagttgggt agtaacgaaa
     1981 tcagcatctt tcaatgcttc gcggcgatcc agcgttttat aaagcttcat cgggacgcca
     2041 gcgttatcaa tcatccgttg gcagagttca aaaatgatat ccagtttctc tttaccatct
     2101 tcgacatcca ccagccataa ttcgctgacc ggcaattcgt gataacgctt aataaatcct
     2161 tccagtaact ccggggtata gctgctcccg ccaccaatag tgacgacttt taatttctgg
     2221 ctcataattt ctcccttcag tacagaatac tgatatctgg catatctgcc cccccggaca
     2281 taaataatcc agcaacagga cagatatgtg aattgtcagg tataacgact tactgattaa
     2341 attcagtcaa tttcttacga tagctcttag gcgtaaagga cgttaatttc ttaaacgttt
     2401 taataaacaa actcgggcta ctataaccgg cctcaaacgc aatatccgtc acggaatagt
     2461 tggtcatttc cagttgtttt ttggcaaaat taatacggat ctcattaata atctgcattg
     2521 gtgttttgcc ataatatcgt tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca
     2581 acgtcaccat attctccagc gccgattcac taaactgctc tttatcatgc atcttttcta
     2641 ccgtactttt cagccactgc ggtacatcat caatcacctg ttcttcgcgg taatgacgta
     2701 aacggttaat gacataaaaa gtaaccatct caacaaactc ttccagccct gtttcgcgga
     2761 aattcaatga agaaatcact gtttccacat aggtgagaaa agcgttattg gtccggtata
     2821 cctgcgaagc gacaaagcaa taaggcaaca acggcaggta atgctgctca aaaaagcgtt
     2881 tactgatccc aacgttcaat atacgcgtgg caccaaactc ataaaaactt tgatggtgcg
     2941 aacctaacgg aataaaaaca aaatcgcccc gttccagtaa cacgcgctta ccgttaatct
     3001 cctggaaata acgcccggtt aataccagag taaattcata atagtcgtgc tgatgcagcc
     3061 cgctgatact ctcagttttg ttatagataa acacatggaa gtttttgcca ttaaacaact
     3121 gctgttctcg ggcagtggca atttccggcg cgttaatcac tggctgcatc atcgactcct
     3181 tatgccttca gtttttcatg aagctcaatt aattcagtaa tcagttcacg cgcaagcatg
     3241 gacgtcatta aatgatcctg agcgtggacc agcaccagac tcactttcat cttaccttcg
     3301 cccgcatcgc cttcaatcag tttcgtctgt accagatgcg cttcattcaa tgccatccgt
     3361 gactgatcca tcatggcttt tgctgcagca aaatcgccct gcttcgcctg tttcagtgcc
     3421 gcatacgcca ggctgcgcgc ttgtccggag ttgatgataa gccccatcac cacttcttcc
     3481 agctcttcag cttctgtttg cgtatcagga atattatcga gatccatcat acatcgttcc
     3541 tctctcttct taccggcaag attacccgta ccggcatcga ttaaaatttc agggcgttag
     3601 cgatatcttc ttcgctctct tctttatcaa tcgcattttg tgctttgtta gccaccacaa
     3661 caaagggcag ataaattaag gttgcgatgc caaggttgaa gagtgcgacc agcaatgcgg
     3721 cgacgctacc gttggtgtta aagaaggctc ccagaccggt tggcatggtc cacggtgcaa
     3781 tattggtcac cggaggaatg atgcccatgt agtacgctgc gagggtgatt gccgccagaa
     3841 tcggttgtac cagtacaaac gggataaaca tcaccgggtt catgataatt ggcagaccaa
     3901 acagaatcgg ttcgttaatc tggaagatgc cggacggcag cgccagtttt gccacctgac
     3961 gataatcagc acggcgagag gcgataaaga tagccaggat caggcctaaa gtcgcaccac
     4021 tgcccccaag gaaaataaag gagtccagca tcggcttggc ccagatatgg aaggtcttac
     4081 cggctgccag cgccgcttcg acggaaccat attgctgata ggtcgcgata ttttccagcg
     4141 cccacggcgt cataatgccg ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga
     4201 agaaccagag cagtggaaca aagatcacat aggcccagcc caccacgcta cccaacgatg
     4261 ccagtggggt tgagatggta tccataatga tctgatggaa gttggtgccc caggtattca
     4321 acgcccaggc aataatcccc atcacggaaa gaataataaa gccgggaatt aatgccgaga
     4381 aggaacgcga tactgaagca ggtacgctgt cgggcagttt aatgacccaa ttgcggcgga
     4441 caataaaggt aaacatttct gccaccacca ggccaataat aatcccggag atgatattcg
     4501 ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc gacactatat ggggtgacgg
     4561 tcataaatgc tgcaacggat aacaacccag ccgccagcgc atcgacttta cgctcttctg
     4621 ccagcgccat gccaataaag aacggtgcca ttaaagacat tatccctaat gttccgttat
     4681 atacgttgcc gccaatacct ttcaggccat taagtgtttc aatggttgag gcatctaggc
     4741 gaatacctaa ggaataaaaa aatgacccct ccccaaagct tagaaaaacg ttgttaatta
     4801 atacaaacat ggcccccgca agggttaacg gcattaagcg aataaagcca tttttgattg
     4861 cattaacgtg tggctgcttt cctattttaa ctgcaaaagg gaggagtacc ttttcaagcg
     4921 atgcaataac attactcata gaaaaatacc cttaaaaacc gcaatttaaa tattgcggta
     4981 ttgatttatg aaataactct ttgacgggaa aatttaaaat aaattaattt gctgcggctt
     5041 ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc atcgactttg ccataaagca
     5101 gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat
     5161 aagcaatctg cggccctaat aacacgacat cggcattctg acctttttca ccagccagtg
     5221 tttccggaaa tgcttcaata atgaccggaa cttcatattt ttctgcctgt gcgcgcattt
     5281 ttgataccag taaagaggta gacatgcccg cagaacaaaa cagataaatg tgtttctttt
     5341 ccataaaact gccctcatcg acgattatct gtcagccaga cactccgcaa gccttaacct
     5401 gcttccatgc tctgggtaac ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt
     5461 atacggtata gacctgagca atagtatttc tttgacctta taaattgttt ctccttgacc
     5521 tgtggttatg accctcttca catttcgggc aaataattcg cgaagataat taagaactca
     5581 ggccaaaaaa aaccggcgca atggccggtt tccgttgtta ctcaagcttt cagacgaatt
     5641 gattacttcg cagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg
     5701 atgacatgtc cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga
     5761 cccaggatgt aggtctggca agtaccgcga gcatggatca tgctcacttc agacgaaggt
     5821 ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca
     5881 ggctgtacaa actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat
     5941 accgccgctg cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat
     6001 cagcgtgtta gataagcctg gaatacattg ggcgcttttt caagcccgtg aacgaaacgg
     6061 ctccgctttc agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg
     6121 tcgtttcagt agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt
     6181 caatgacatt gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgctg
     6241 aagaggaaat tcgtcgtagt atcgattttc tgaaaagcta cctgcaaact tatccgttca
     6301 ttaaatcact ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt
     6361 gccagatggc gattaatgag ctgcgcctgg aaaccggcaa cgaatcactg caatttattg
     6421 ccgtacgcct gccctatggt gttcaggccg acgaacaaga ttgccaggat gccattgcct
     6481 ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg gccagcgaac
     6541 aggcattgcg ggaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc
     6601 gtgagcggat gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtgggca
     6661 ccgatcatgc agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacag
     6721 acattaatcc gctgtatcgt ctcaacaaac gtcagggtaa acagttactg gcggcattag
     6781 gttgcccgga acacctttat aagaaagcgc caacggccga tctggaagat gatcgccctt
     6841 ctctgccaga tgaagtggca ctcggcgtga cctatgacaa tatcgacgac tatctggaag
     6901 ggaaaaacgt acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac
     6961 ataaacgccg tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac
     7021 ataatggttt gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg aacgccttat
     7081 tcgacctaca aaactatgca aattcaatat attgcaggag tcgaataggc ctgacaggcg
     7141 tagcacttca gacggtgtaa cctttgtcat cgacccgcct cttttttaat cgcttcccgc
     7201 ctgttacact ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct
     7261 ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc
     7321 cttaatgact tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg
     7381 ccgctctata tcggcaaaag cgttaatatc cgcagtcggg tgctttctca tttacgcacc
     7441 ccggatgaag ccgccatgct acggcaatcc cggcgaatta gctggatctg caccgcaggc
     7501 gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat
     7561 aaacggttgc gccgcaatcg ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat
     7621 gtggtgtatg ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc
     7681 aataggcgcg cagctttgca agcattgcag agcatcgctg atgaacaaaa actttgttat
     7741 ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa
     7801 cgttgcgccg gagcatgctg cggtaaagag agccatgagg aacatgcgct acgcttgcgc
     7861 cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc aaggtgcggt ggcactgaaa
     7921 gaacagcacc cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg
     7981 gttaattcgc tggaagaagc gacaacgtta attcggacac ccgccgggtt tgatcacgac
     8041 ggttataaaa ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat
     8101 ccggcgaatg accagcgagc cagttgattt cactgaacaa cagtttacct tgcggttgta
     8161 aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcaatt
     8221 tatcgcccag ttgccacgcc ccgttaatca caaacaccac cccgccgcgt gagccaaagg
     8281 tagtaaacgt acgttcggca attctgactt tcgccttaca gacatccagg cgtgtcatga
     8341 tgttgaaatc catcgacatc tgccctgccg tcagtttcgc ttttaccacc tggtccgctg
     8401 caaaggcaaa aggctgtaac ggttttaagg tatggttaaa gcggtctgcg ctttcaagga
     8461 acatctcgcc gccttccagc aacgtcacta tcctttccat gccgggaaac aaagaaaact
     8521 ccccattcgc cgcgatggat gcaatgctgg cgcgccagta aaaatcacgt tttgccggtg
     8581 ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg ccacagattc accgacattt
     8641 tacgcatatc aaagtattcc atactcgctc ccggcccgtt cttttcattg ttatcgttca
     8701 ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg ttagtggtga tcaggctttt
     8761 tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa
     8821 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca
     8881 ggcattttac cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat
     8941 tgctttttct gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc
     9001 agcatgttag ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta
     9061 tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc gttcttccag cggcggacgt
     9121 ttcatctggt cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc
     9181 aggttcaggt ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcatc
     9241 atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc
     9301 gcgccaagag ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc
     9361 cttcctttca gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac
     9421 tttactgact ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac
     9481 acttttgcgc caattatgga gaaaaaaaga aaatttgatg gagagtgatg agagaataat
     9541 acaacacgat gattttgcag agattatgaa gaactatacc ggatgactgg tgataaataa
     9601 agcaaataac caggattaat ctgtattaat ttataagaaa gcaacttaat acccgcagaa
     9661 tgatttctgc gggtaagtat tagcttattt tttcgagcat taatcccgcg cgtaatccca
     9721 acgctaccaa cggattaggg aataacacat actctacatc atgggttacg gtaaaacgtt
     9781 cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg cataaaattc agcgtgtcac
     9841 ttgccatatg catttcgaag gacggcgagt ggcgagtaat ttgcgaaacc acccgataac
     9901 ggagcggcgg tgttctcacg ataccgacac tctcaccaga tagcagcgca gcaattgcgc
     9961 tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt
    10021 ccagcgtaca ggccagcgcg ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag
    10081 gttcctgatg gaacaccagc gcctccagcc ccgccgcacc cagccacgtc agaaatttct
    10141 cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg cggatgcaag gagccacgaa
    10201 ttgcggtatg tagatcaagg tgccagcgca cagattcttt gcccaggtca taaaaatctt
    10261 ccaggcactg ttccagttcg cgcgcccgac aggtttctcc gctttcagca aatagctgcc
    10321 aacgaccgcc aaacattcga ttcatatcgc tatggcaata acgtttccct tgcttcagcg
    10381 caggaggatt cccgaggatc accagcaacc gccaacgtaa cgggatctcg ccgtgagata
    10441 acgcgccaag caacgcgtcc agcatctcca caggtgccgt ctcattaccg tgtattcccg
    10501 ctgaaatcac cagtgcgcct tgcggtggcg ttaatggcgt cagttccagc acaccatcgc
    10561 ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt gataaccggt tttttacccg
    10621 ttaaggtcag agcaagaaaa ttatccattg ccccctccct cgcgctggaa cggataaacc
    10681 gaaccgagat tcagtaattg gctcaataca tccagtgctt cccgccctcc gcgcagcaat
    10741 tgcgggtcgg ccagatcggc agcagtaagg cgatcgcggt agtaacgatc cacccagtca
    10801 ttgagcgcat taaacagcgt atcgttcatc atcaccgccg gattcaccgc ccggcgttct
    10861 tcttctgtca ataccacccg caaccgcagg cacgccgggc cgccgccatt cgccatgctt
    10921 tcacgtaaat caaagacttt tagttcgcta atcgggttgt cagcggcaag gagttcattg
    10981 agataacccc atactccggc gtgttcccga cactcctgag gcagcaccaa catcatggaa
    11041 ccatcatcgc ggctcaacag ttggctgtta aacagatagg tagacaccgc atccgacacg
    11101 gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt tcacccgcgc acgcaggttt
    11161 gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac ctggcggtta
    11221 ctcacggcaa tcacgtcatt atgaaaaacg ccctggtcga taacgtccgg gttttgctgg
    11281 gcgaaaatca cctgttgggg attcacctga ttcagccttg ccaccgcctc gctggcttcg
    11341 cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc tcgcccgtag
    11401 acaaaaagtt gcatacccgg ctcaccgtaa tgaccgccga gacgattgtg gtttgccgcc
    11461 ccctcatcac cgagcaacgc tacctgcggc aacgccgaat gaaagctaaa tttctcttcg
    11521 tcgttaaaaa tcgcttttaa aagtgattcg gtgacgtgcg cttccagcga gcggtgaaat
    11581 ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc ggcagatggc
    11641 gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct ggaaagccag
    11701 tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact gaatcccaac
    11761 tgacgcagca ccggaataaa cggacgctcg tgcggcggga tcacggcctg ggggaatccc
    11821 gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgcctgtcg cgggttagac
    11881 acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggccagc gtaatgatgc
    11941 gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc acctcatcgg
    12001 aaaaatccag cccggggtta agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg
    12061 ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg ttaccggatg
    12121 caccaatgcc gccgaatggc gcggtactgg cagcaccagt aagcggtttg ttccagttaa
    12181 caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg ggggaaacca
    12241 gaccgcaaga gagtccgaag cgagtgttat tcgccattag aatcgcttcc tcgaaagaat
    12301 cataacgcca gacgcgcaat aacggtccga acacctcttc atctggtacg ccagcaacgc
    12361 ctgtcatttc aatgatcccc ggcgtcagca acgatgtctc tgattgtaat aagcgcggcg
    12421 caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc acctgctgtg
    12481 cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg
    12541 gcgttaatcg ctggctgacg gcaaccagac gagcaagaaa cgcatcgccc tgcgctccgc
    12601 ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca aacgccgact
    12661 gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt agcggattat
    12721 taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg cgatgcagct
    12781 ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca
    12841 gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag tacgcccggc ggcaagccag
    12901 cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg ctgggtttaa
    12961 agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc aaatgaccag
    13021 ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc aggctcgccg
    13081 caccgtccgg catttcacta cgctgctcgc cggtacgagc gtgatacgcc ttaattgata
    13141 tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc ggcttgccgg
    13201 tttctcgggc aatgattgcg gttaattcgc ctttattcct ttccagcaat ccggcaaagc
    13261 gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga aacgccgcac
    13321 gggctgcccg acaagcctgc tcgacctgag cggcatcggc atcattgcct tgccataaca
    13381 catcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac
    13441 cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg
    13501 atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt
    13561 tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat tggcgaccag
    13621 gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca gccgactttt
    13681 acgaatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc
    13741 gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg
    13801 cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg
    13861 atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcggc
    13921 gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg gtgaatagcc
    13981 gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact tgtcgcgaaa
    14041 agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca
    14101 gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa
    14161 cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca acgtgccgac
    14221 gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatggcac aaatccccgc
    14281 cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcac ttttgggcag
    14341 ttcgccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc
    14401 gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc
    14461 tgatcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa
    14521 gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt gagcgcaggc
    14581 gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt cgccgcttcc
    14641 tgagagatct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc aatcagcaaa
    14701 cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt aagacgctca
    14761 acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc
    14821 agcactttgc ctgccaccgc cgaggccagc ggattaccgc cataggtggt gccatgagtg
    14881 ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac cgggaaaccg
    14941 ccgcccagcg ctttggcggt agttaacaga tcaggcgtca cgccgtagtg catataggca
    15001 tacagttccc cggtgcgccc gacgccggtt tgtacttcat caaaaatcaa cagcgcattg
    15061 tggcggttac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg caccacaccg
    15121 ccttccccct ggatgggttc gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg
    15181 gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg tgcaaaatcc
    15241 tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc atgaaacgca
    15301 tttttgaacg ccacgatgcc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc
    15361 gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg
    15421 gcaaacgtgg catcgatcaa ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg
    15481 ttgccggtat gccagaactt actcgcctgt tcgttcagcg cctcacgcag ttccggatgc
    15541 gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttcccctgc
    15601 tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg agcgtaaaca
    15661 ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca
    15721 gtaaataatt cgttatttat atgttaacaa taagtaatgt ttgcgctgta aatgtagatt
    15781 gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa ctggaaatca
    15841 aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac gtttgcgatg
    15901 tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa
    15961 ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca gcgcgcacca
    16021 aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta
    16081 acaacaggcg gtaagcaacg cgaaattctg ctaccatcca cgcactcttt atctgaataa
    16141 atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac
    16201 cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa
    16261 gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa cgtgttttat
    16321 cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc gattgccgtg
    16381 cgtcgcggtt ttcccggtga cgacgaagaa gcgcagcggc gaattattat ggcggaaatc
    16441 ccctcgccgc tgggcaatgt caccgtgatc aacggttact tcccgcaggg tgaaagccgc
    16501 gaccatccga taaaattccc ggcaaaggcg cagttttatc agaatctgca aaactatctg
    16561 gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa tatcagccct
    16621 acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa
    16681 tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg ggggttggtc
    16741 gataccttcc gccatgcgaa tccacaaaca gcagatcgtt tctcatggtt tgattaccgc
    16801 tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg
    16861 ctggcagaat gttgtgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg
    16921 tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcagctc tcctggctca
    16981 aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg
    17041 cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat
    17101 caagtttgat ataggaaact ccacgatgaa cgctgagcgt aaatttcttt tggcctgtct
    17161 tatttttgcg ctggtcattt acgctatcca cactttcggt ttattcgatc tgctcaccga
    17221 tttaccccac ttacagacac tcatccgcca gagcggattg ttcggttata gcctctatat
    17281 tctgttattc attattgcca ctctctttct gttaccagga agcatactgg tgatcgctgg
    17341 aggaatagtt tttggcccgc tcttagggac actactctca ttaattgccg ccacgctcgc
    17401 ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt gatttactgc tgaaatacgt
    17461 tggtcatagc catacctttc aggccattga aaaaggcatt gcgcgtaacg gtattgattt
    17521 tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg
    17581 attaaccaca atcgccttct ggccttatac ccttatttcg gcactcacga ccctgcccgg
    17641 tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgcgctt
    17701 tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact
    17761 ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac ttactcaccc
    17821 aaaaaatgaa ggatagaacg atgtcgcaac attattcagt gtcatggaaa aaaggactgg
    17881 ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg
    17941 cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgta gatgccaata
    18001 accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac gacactcgtc
    18061 acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg gcttatgcga
    18121 ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc gaaaacatga
    18181 cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt tcggtcaact
    18241 ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt aatggcaaga
    18301 aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa acgggttgcc
    18361 tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac acttatggtg
    18421 cggtggaaaa acgcggcgaa gtgaaattca aaggcaatgc ctcagttctc ccggcggata
    18481 acacgctggc aacggttacc tttaaaatca ccgaataaag ccaggataaa ggatgatgaa
    18541 gatacaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt tgttcgctat
    18601 gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca gcgttgcggc
    18661 gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg gcgcactggc
    18721 ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc tacctgcgtt
    18781 tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct tgctgtcgtg
    18841 gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga tgggccgcga
    18901 agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt atggacggct ttttcactcg
    18961 ctacggcaaa cacaccatcc tggtctgtcg gttattgcct tttgtccctt tcgatccaat
    19021 cagctatgct gccggtctga cttcaatacg ttttcgctcg ttttttatcg ccaccgggct
    19081 tggtcaatta ccggcgacta ttgtttattc ctgggcgggc agcatgttaa caggcggtac
    19141 tttctggttt gtcaccggac tgtttattct gtttgccctg accgtggtga tttttatggc
    19201 gaagaaaata tggcttgagc gccagaagag gaatgcctga tgggtttacc gccgcttagc
    19261 aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg
    19321 ctaagcccga ttcgctggtg ggggcggatc ccgtttatct tttatttggt gtcaatgttt
    19381 gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc gcttgtcagc
    19441 gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc
    19501 gccgagcgta ccggcagcac cgataaactg ctggcagtgg ctgactggcg gcaaagcccg
    19561 ctcttcagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag cgtaacgccg
    19621 ccaacggtcg atgatgccct gcgcacccga ctggctgcgc attttgacgc tcaggcgctc
    19681 accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg
    19741 gacattcccg ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg agagaagatg
    19801 cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctctggcaac acatgccagt
    19861 gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc
    19921 ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa aacccattac
    19981 gctataaacc tgaagattgt tcgtctggcg gatgccgcag acgcggtgaa gcgcattcag
    20041 accgaagctg ctgccggacg taaaacgggc ggctcggtgg atctgctctg ggtgaacggc
    20101 gaaaacttcc gcaccttaaa agaggccaat ttattacaaa tgggctgggc ggagactctg
    20161 cccaactggc gctatgtcga cacacagctg ccggtgcggg aagatttttc agtgcctaca
    20221 gaaggggctg aatcgccctg gggcggcgca caactgacgt ttatcgcccg ccgcgatgtt
    20281 acgccacagc caccacaaac gccgcaagcc ttactggagt ttgctaaagc caatcccggc
    20341 acggttacct atccgcgccc accggacttt accggcacgg cgtttcttga acagttgctg
    20401 attatgctga cgcccgatcc cgccgcgtta aaagttgccc cggataaagc cactttcgcc
    20461 cgcgtcactg cgcccttgtg gcaatatctt gacgcgctac atccctattt atggcgcaaa
    20521 ggaaaggatt tcccgccttc gcccgcgcgg atggatgctc tgctgaaagc cggcacattg
    20581 cgcctgtcgc tgacctttaa ccctgctcat gcccagcaaa aaatcgccag cggcgatttg
    20641 cctgcaagca gttacagttt tggttttaac caggggatga ttggcaacgt gcatttcgtc
    20701 accattcctg ctaacgcaaa tgccagtgct gcggcgaagg tggttgccaa cttcctgctc
    20761 tcacccaatg cgcaactgcg taaagcggat cccgctgtct ggggcgatcc ttctgttctc
    20821 gatccgcaaa aactgcctga tgggcagcgc gaaacattgc aatcaagaat gccgcaagat
    20881 ctgccgccgg tactggctga accgcacgca ggttgggtaa atgcgctgga acaagaatgg
    20941 ctacaccgtt acggtacgca ttaatctttt tgctgtgggc gatggtggcg gtgatttatg
    21001 caccactgat cccggcagct ctcacgctga tatcgcctgc tttgtcgttg acacactggc
    21061 aggcgttatt tgccgatcca cagttaccgc aagcattact ggcaacgctg gtgtcgacaa
    21121 ccatcgcggc ggtcggggcg ttgttgattg ccctgctggt gattgtggcg ctgtggcctt
    21181 ggccgaaatg gcagcgtatg tgtgcccgtc tgccgtggct gctcgccatt ccccatgtgg
    21241 cttttgccac cagcgccctt ctgctctttg ctgacggagg gctgctttat gactatttcc
    21301 cgtatttcac tccgctaatg gacaaattgg gtattgggct gggcctcacc cttgcagtga
    21361 aagaaagcgc atttctgctg tggatcttag cggcagtatt gagcgaaaaa cggctgttgc
    21421 agcaggtcat tgtgctggat tcgctgggct acagccgctg gcaatgcctg aactggctgc
    21481 tgttgccctc cgtcgcacct gcactggcaa tggcgatgtt ggcgattgtt gcctggtcgc
    21541 tttcggtcgt ggatgtggcg attattcttg ggccaggtaa tccaccgacg ctggcagtaa
    21601 ttagctggca gtggttaact cagggcgacg cggatcaaca aacgaaaggc gcactcgcca
    21661 gtctgctgct gatgctgtta ctcgccgcct acgttttgct gagctatctg ctatggcgca
    21721 gctggcggcg cactattccc cgcgtagatg gcattcgcaa gcctgccaca cctttattac
    21781 caggcactac gctggcgagt tttttaccct taaccggtgt gctgtgtgtg gttctgctgg
    21841 cgatcctcgc ggatcagtcg acgatcaata gtgaagcact catcaacagc ctgacaatgg
    21901 ggctggtggc gacattcatc gctttgctcc tgttactgct gtggctggaa tgggggccac
    21961 agcgtcgcca gttgtggctc tggttgccca ttctattgcc cgctctgcca ctggtggcgg
    22021 gccagtacac gctggcgcta tggctgaacc tggatggaag ctggacggcg gtggtctggg
    22081 ggcatctgct gtgggtaatg ccgtggatgc tgtttatcct gcaacccgcc tggcagcgca
    22141 ttgattcacg gttaattttg attgcgcaaa cactgagctg gtcgcgggcc aaaatcttct
    22201 tttacgtgaa atgtccgctt atgctacgcc ctacgctgat tgccttcgcg gtgggatttt
    22261 cagtcagtat tgcgcagtat atgccgacgc tgtggctggg cgcggggcgt tttccgacgc
    22321 tcaccactga ggcggtggca ttaagcagcg gcggcagcaa tggtattttc gccgcccagg
    22381 ctttatggca actgctatta ccgcttatta tttttgccct gaccgcgtta gtcgcaaaat
    22441 gggtaggtta tgtcagacaa ggactccgct aatgctctgc gtgaaaaatg tttcgctacg
    22501 tttaccagaa agccgcttgc tgacaaacgt taactttacg gtggataaag gtgacattgt
    22561 cacgttaatg gggccgtctg gctgtggaaa atccactctg ttttcatgga tgattggtgc
    22621 actggctgga cagttttctt gtacaggtga gctatggctc aatgagcaac ggattgacat
    22681 gctacccacc gcacagcgtc agattggcat tcttttccag gatgcactgt tatttgacca
    22741 gttcagcgtc gggcaaaatt tactgctggc gctaccgtcg acgctcaagg ggactgccag
    22801 gcgcaatgcc gtgaaagacg cgcttgatcg agcgggactg gctgaaactt atcaccagga
    22861 ccctgcgaca ttatcaggcg gtcagcgagc gcgggtggcc ctgctgcgcg cccttctcgc
    22921 tcagccaaaa gcgttactcc tggatgagcc attcagccgt cttgatgtgg ctctgcgcga
    22981 taattttcgc cagtgggtgt tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt
    23041 aacgcacgat ctccaggatg ttcctgctga tagttctgtt ctggatatgg cgcagtggtc
    23101 agaaaattac aacaaactgc gataacgcaa agtttttctc aatgcgtcag ttcagaatgg
    23161 cgcactcaaa actacaatgt cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct
    23221 ggcaatggct ttagggctgg cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct
    23281 tacacttgac cagcttcaac aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt
    23341 ttataacggc tggccacaaa ccttaaatgg cccttctggt catgaacctg ccgccttaaa
    23401 cctctctgcc agctggctcg acaaaatgag caccgaacag ctcaacgcgt ggatcaagca
    23461 acataacctg aaagccgatg ccccggtggc gctgtacggt aatgacaaag atgtcgacgc
    23521 cgtcaaaacg cgactgcaaa aagcaggttt tacgcatatc tccattctga gtgacgcgct
    23581 aagcgaacct tcccgtctac aaaaactgcc gcactttgag cagctggttt atccgcaatg
    23641 gctgcacgac ctgcaacaag gtaaagaggt tacggcgaaa cctgccggtg actggaaagt
    23701 cattgaagcg gcctggggcg ctcctaagct ttaccttatc agccatattc ccggtgctga
    23761 ctacatcgat accaacgaag tggaaagcga accgctgtgg aacaaagttt ctgatgaaca
    23821 actaaaagcg atgctggcaa aacacggcat tcgccatgac accacggtca ttctgtacgg
    23881 gcgtgacgta tacgctggag cgcgtgtggc gcaaattatg ctctacgcag gcgtgaaaga
    23941 tgtgcgtctg ctggatggcg gctggcaaac ctggtccgac gcgggactgc ctgttgagcg
    24001 aggaacgcca ccgaaagtga aagcggaacc ggatttcggc gtgaagatcc cggcacaacc
    24061 gcagttgatg ctcgatatgg aacaggcccg tggactgctg catcgccagg atgcatcgct
    24121 ggtgagcatt cgttcgtggc cagaatttat cggtacgacc agcggttaca gctatattaa
    24181 accaaaaggt gaaatagccg gagcacgttg gggacacgct ggtagcgact cgacgcatat
    24241 ggaagatttt cataacccgg atggcaccat gcgcagcgcc gatgatatta ccgctatgtg
    24301 gaaagcgtgg aatatcaaac cagagcagca agtttcattc tactgcggca ccggctggcg
    24361 cgcgtccgaa acctttatgt acgcacgcgc catgggttgg aagaatgttt ccgtgtatga
    24421 cggcggctgg tacgaatgga gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg
    24481 cccggacagt agcaaataac attgcaatta ctgacgctgg agcgacttca gcgtcagata
    24541 cccactccac acccgcgtaa aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa
    24601 ccacgcaaac catgcaggga ataaacatcc cagcacaaac agtaagatcg tttctgtgcc
    24661 ttcggttaat ccccccagat agtaaaacga cttatgtgca taaccggggt tatcaatctg
    24721 atgttttgct gccagtgcag caaaggcgag aaaactgctg cccgtgccga taaacgcaaa
    24781 caacaaccag ccgcctgcca gcgcattttg ctccggtgca gcaagaataa agccaaacgg
    24841 caccagcgcg taaaagagaa aatcgagaga aatatcaaga aagccgcccg cgtcggtaag
    24901 ctctctgcgc cgcgccagcg caccgtccag accatcaagc agcctgttca acaaaatgac
    24961 gactagcgcc gccagatacc agcccagcgc cagaaacggc agagccagca cgccgatggc
    25021 aaatcccact aatgttaaac catctggcgt aatgcccggt ttatcaagaa cccgcacgca
    25081 ctgatgcaac aacggtttaa tccggggatg aagatggcgg tctagcacag gcactcctta
    25141 aatataaagc ctttctgatt gagcaacagt gcggatatta tggcattttt cgcttatctg
    25201 cccgtgtgta atttatgaaa atgattgaag ttgttgccgc catcattgaa cgtgatggca
    25261 aaattttact cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg gagtttgccg
    25321 gtggtaaagt cgagccggat gaaagccagc ggcaggcgct ggtgcgtgag ttacgcgaag
    25381 aactgggcat cgaagcaact gtgggtgagt atgttaccag ccatcagcga gaagtttcgg
    25441 gccggattat ccatcttcat gcctggcacg tacccgactt ccacgggacg ttacaggcac
    25501 atgaacatca ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat ccgctggccc
    25561 ctgctgacat tccattatta gaggcgttta tggctttacg cgccgccaga ccagcggatt
    25621 agtgctaagg gttttgtcat cccgctggca ttgcagcagt attccctcgg ctttaattac
    25681 cgcgccttca gaataatttt tatcctgata aacgcagcac tgagtacagg gctgcgctga
    25741 ctgcccgcct gaactgaaca cttctggcgg tacgtttacc tccacgtccg gacgataatg
    25801 cgggttagcc agtgcaatta atggaaatgc taatactacg gcgaacaatg ctcgactcac
    25861 agggaagtcc ttaacgttat tgtctctgct actgataacg gtagccgggt ggcaaaactt
    25921 tagcgtctga gttatcgcat ttggttatga gattactctc gttattaatt tgctttcctg
    25981 ggtcattttt ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc
    26041 acatcacata aacaacataa gcacaatcgt attaatatat aagggtttta tatctatgga
    26101 tcagacatat tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac
    26161 cgagttcgcg caagccgttc gtgaagtaat gaccacactc tggccttttc ttgaacaaaa
    26221 tccaaaatat cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca
    26281 gtttcgcgtg gtatgggttg atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt
    26341 gcagttcagc tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa
    26401 cctctccatt cttaaattcc tcggcttcga acaaaccttc aaaaatgccc tgactaccct
    26461 gccgatgggc ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga
    26521 agtgatgcgt ttttgccagg cgctgatgac tgaactgtat cgccacctgg gcgcggatac
    26581 cgacgttccg gcaggtgata tcggggttgg tggtcgtgaa gtcggcttta tggcggggat
    26641 gatgaaaaag ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg
    26701 cggcagtctt attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat
    26761 gctaaaacgc cacggtatgg gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa
    26821 cgtcgcccag tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc
    26881 agactccagc ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct
    26941 tatcgaaatc aaagccagcc gcgatggtcg agtggcagat tatgccaaag aatttggtct
    27001 ggtctatctc gaaggccaac agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc
    27061 cacccagaac gaactggatg ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc
    27121 cgtcgccgaa ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc
    27181 aggcgtactg tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa cgtcaggcct
    27241 tgaaatggcg caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt
    27301 gcatcacatc atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca
    27361 aaccaactac gtgcagggcg cgaacattgc cggttttgtg aaggttgccg atgcgatgct
    27421 ggcgcagggt gtgatttaag ttgtaaatgc ctgat
//
LOCUS       DAMZII010000060        27361 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZII010000060 DAMZII010000000
VERSION     DAMZII010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.060"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..677
                     /locus_tag="POA77_002449"
     CDS             <1..677
                     /locus_tag="POA77_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6365875.1"
                     /translation="NNLLSLSGQNEQNALQITTLESQIQTQAADFDNRIYQMELQRLE
                     LQKELVNTDVEGEIIIRALSDGKVDSLSVTVGQMVNTGDSLLQVIPENIENYYLILWV
                     PNDAVPYISAGDKVNIRYEAFPSEKFGQFSATVKTISRTPASTQEMLTYKGAPQNTPG
                     ASVPWYKVIATPEKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWMLSPFYDMKHSA
                     TGPIND"
     gene            652..2766
                     /locus_tag="POA77_002450"
     CDS             652..2766
                     /locus_tag="POA77_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HDI6365876.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDREL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVCVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMGPISLETNGLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQI
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMN
                     ITRVIIAHRETTLRTVDRVISI"
     gene            complement(2936..3247)
                     /gene="cvaC"
                     /locus_tag="POA77_002451"
     CDS             complement(2936..3247)
                     /gene="cvaC"
                     /locus_tag="POA77_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HDI6365877.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVC
                     L"
     gene            complement(3225..3461)
                     /gene="cvi"
                     /locus_tag="POA77_002452"
     CDS             complement(3225..3461)
                     /gene="cvi"
                     /locus_tag="POA77_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HDI6365878.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            3622..4399
                     /locus_tag="POA77_002453"
                     /pseudo
     CDS             3622..4399
                     /locus_tag="POA77_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            4401..4670
                     /locus_tag="POA77_002454"
     CDS             4401..4670
                     /locus_tag="POA77_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HDI6365879.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            4667..5647
                     /locus_tag="POA77_002455"
     CDS             4667..5647
                     /locus_tag="POA77_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDI6365880.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESIRLNTEVV
                     SLRKDAGHRVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASSLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPQAERFTGEGAIKFVDASQEKYDVIITCTGYEMPDYSFIRKRTA"
     gene            5723..6103
                     /locus_tag="POA77_002456"
     CDS             5723..6103
                     /locus_tag="POA77_002456"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI6365881.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            6100..6447
                     /gene="tnpB"
                     /locus_tag="POA77_002457"
     CDS             6100..6447
                     /gene="tnpB"
                     /locus_tag="POA77_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6365882.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            6497..7771
                     /locus_tag="POA77_002458"
                     /pseudo
     CDS             6497..7771
                     /locus_tag="POA77_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            7811..8077
                     /locus_tag="POA77_002459"
                     /pseudo
     CDS             7811..8077
                     /locus_tag="POA77_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(8064..8515)
                     /gene="def"
                     /locus_tag="POA77_002460"
                     /pseudo
     CDS             complement(8064..8515)
                     /gene="def"
                     /locus_tag="POA77_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            complement(8624..12757)
                     /gene="hbp"
                     /locus_tag="POA77_002461"
     CDS             complement(8624..12757)
                     /gene="hbp"
                     /locus_tag="POA77_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-binding protease autotransporter Hbp"
                     /protein_id="HDI6365883.1"
                     /translation="MNRIYSLRYSAVARGFIAVSEFARKCVHKSVRRLCFPVLLLIPV
                     LFSAGSLAGTVNNELGYQLFRDFAENKGMFRPGATNIAIYNKQGEFVGTLDKAAMPDF
                     SAVDSEIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVDRNNAPSLDFHAPRLD
                     KLVTEVAPTAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTKMGGAYSWLT
                     GGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEGGALEWSFNSSTGAGA
                     LTQGTTTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSN
                     GSTWTGAGIVVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVL
                     NQQADNKGQVQAFSSVNIASGRPTVVLTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQ
                     LKAADYGAVLANNVDKRATITLDYALRADKVALNGWSESGKGTAGNLYKYNNPYTNTT
                     DYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNTAGYLFHGQLKGNLNV
                     DNRLPEGVTSALVMDGAADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHS
                     VLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTIQADNSSVTLGDSRVFI
                     DKNDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVN
                     ISSDSAVLGNSTLTSTALNLNKGANALASQSFVSDGPVNISDATLSLNSRPDEVSHTL
                     LPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQML
                     YSLFNGYRNIWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTT
                     LTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSNKDTLDIPLVS
                     APEATADNLFRASTRVVGFSDVTPILSVRKEDGKKEWVLDGYQVARNDGQGKAAATFM
                     HISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADR
                     KHELGSMDLFTGVMATYTDTDASADLYSGKTKSWGGGFYASGLFRSGAYFDVIAKYIH
                     NENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNW
                     NDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKD
                     AAGEHQINGRKDSRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF"
     gene            12928..13119
                     /locus_tag="POA77_002462"
                     /pseudo
     CDS             12928..13119
                     /locus_tag="POA77_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(13087..13302)
                     /locus_tag="POA77_002463"
                     /pseudo
     CDS             complement(13087..13302)
                     /locus_tag="POA77_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            13398..13655
                     /locus_tag="POA77_002464"
                     /pseudo
     CDS             13398..13655
                     /locus_tag="POA77_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13723..14739
                     /locus_tag="POA77_002465"
                     /pseudo
     CDS             13723..14739
                     /locus_tag="POA77_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            14786..15999
                     /locus_tag="POA77_002466"
     CDS             join(14786..15077,15077..15999)
                     /locus_tag="POA77_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6365884.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNNRRLLGRLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    15067..15183
                     /locus_tag="POA77_002466"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            16044..16187
                     /locus_tag="POA77_002467"
                     /pseudo
     CDS             16044..16187
                     /locus_tag="POA77_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            16309..16674
                     /locus_tag="POA77_002468"
     CDS             16309..16674
                     /locus_tag="POA77_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6365885.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVTPPV"
     gene            16674..16936
                     /locus_tag="POA77_002469"
                     /pseudo
     CDS             16674..16936
                     /locus_tag="POA77_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706936.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            17141..18152
                     /locus_tag="POA77_002470"
                     /pseudo
     CDS             17141..18152
                     /locus_tag="POA77_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18730..19320
                     /locus_tag="POA77_002471"
     CDS             18730..19320
                     /locus_tag="POA77_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6365886.1"
                     /translation="MSRIFAYCRVSTLEQTTENQRREIESAGFNVRPQRIIEEQISGS
                     VAASERPGFNRLLDRLESGDVLIVTKLDRLGRNAMDIRKTVEQLTESGIRVHCLALGG
                     VDLTSSAGKMTMQVISAVAEFERDLLLERTYSGIARARDAGKRFGRPPLLNEEQKQKV
                     LERIKSGASISAIAREFRTTRQTILRVRAKQQTVTV"
     gene            19367..19525
                     /locus_tag="POA77_002472"
                     /pseudo
     CDS             19367..19525
                     /locus_tag="POA77_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19675..20673
                     /locus_tag="POA77_002473"
     CDS             19675..20673
                     /locus_tag="POA77_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6365887.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGVCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            20673..21710
                     /locus_tag="POA77_002474"
     CDS             20673..21710
                     /locus_tag="POA77_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6365888.1"
                     /translation="MSVGAIETSRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGSGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTTASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            21710..22471
                     /locus_tag="POA77_002475"
     CDS             21710..22471
                     /locus_tag="POA77_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6365889.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVACVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            22528..23715
                     /locus_tag="POA77_002476"
     CDS             22528..23715
                     /locus_tag="POA77_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365890.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(23793..24050)
                     /locus_tag="POA77_002477"
     CDS             complement(23793..24050)
                     /locus_tag="POA77_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI6365891.1"
                     /translation="MEKKTISDYTEAEFLEFVRKICRAEGETEADDDKLVDEFRRLAE
                     HPDGSDLIYYPRDDREDSPEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(24051..24284)
                     /locus_tag="POA77_002478"
     CDS             complement(24051..24284)
                     /locus_tag="POA77_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6365892.1"
                     /translation="MEVGKDPELLKQFKNQNKVLVTKGKSSFVPESERVGERERFELH
                     HIKRVTDGGAVYDIDNLRVVTPKHHIEIHRGNK"
     gene            24380..24651
                     /locus_tag="POA77_002479"
                     /pseudo
     CDS             24380..24651
                     /locus_tag="POA77_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24812..24991)
                     /locus_tag="POA77_002480"
                     /pseudo
     CDS             complement(24812..24991)
                     /locus_tag="POA77_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(25037..25204)
                     /locus_tag="POA77_002481"
                     /pseudo
     CDS             complement(25037..25204)
                     /locus_tag="POA77_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            25201..26031
                     /locus_tag="POA77_002482"
                     /pseudo
     CDS             25201..26031
                     /locus_tag="POA77_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
     gene            complement(26075..26723)
                     /locus_tag="POA77_002483"
                     /pseudo
     CDS             complement(26075..26723)
                     /locus_tag="POA77_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            26780..27211
                     /locus_tag="POA77_002484"
                     /pseudo
     CDS             26780..27211
                     /locus_tag="POA77_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
BASE COUNT         6784 a         6932 c         6942 g         6703 t
ORIGIN      
        1 aaaacaacct tctcagtctg agcggacaaa atgaacaaaa tgccctgcag ataaccactc
       61 tggagagtca gattcagact caggcagcag attttgataa tcgtatctat cagatggaac
      121 tgcaacgact cgaattgcag aaagaactgg ttaacactga tgtggaaggc gaaatcatta
      181 tccgggcgtt gtctgacggg aaagttgact ccctgagtgt cactgtaggg caaatggtca
      241 ataccggaga cagccttctg caggttattc ctgagaacat tgaaaactat tatcttattc
      301 tctgggtccc gaatgatgct gttccttata tttcggctgg tgacaaagtg aatattcgtt
      361 atgaagcctt cccctcagaa aaatttgggc agttctctgc tacggttaaa actatatcca
      421 ggactcctgc gtcaacacag gaaatgttga cctataaggg agcacctcaa aatacgccgg
      481 gtgcctctgt tccctggtat aaagtcattg cgacgcctga aaagcagata atcaggtatg
      541 acgaaaaata cctccctctg gaaaatggaa tgaaagccga aagtacacta tttctggaaa
      601 aaaggcgtat ttaccagtgg atgctttctc ctttctatga catgaaacac agtgcaacag
      661 gaccgatcaa tgactaacag gaatttcaga caaattataa atctgcttga tttgcgctgg
      721 caacgtcgtg ttccggttat tcatcagacg gagaccgctg aatgtggact ggcctgccta
      781 gcaatgatat gcggtcattt tggtaagaat attgacctga tatatcttcg ccggaagttt
      841 aatctctctg cccgtggagc aacccttgca ggaatcaatg gaatagcgga gcaactgggg
      901 atggccaccc gggctctttc actggagttg gatgaacttc gagtcctcaa aacgccgtgt
      961 attctccact gggatttcag tcacttcgtc gttctggtca gcgtaaagcg taaccgttat
     1021 gtactgcatg atccggccag gggcataaga tatatcagcc gggaggaaat gagccgatat
     1081 tttacaggcg ttgcacttga ggtctggccc ggaagtgaat tccagtcgga aaccctgcag
     1141 acccgcataa gtcttcgttc actgattaac agtatttacg gtattaaaag aacgctggcg
     1201 aaaattttct gtctgtcagt tgtaattgaa gcaatcaatc tgctaatgcc ggtggggaca
     1261 cagctggtta tggatcatgc tattcctgcg ggggacagag agctactgac gctaatttct
     1321 gctgctctta tgttttttat attactcaaa gctgcaacga gtacgctgcg cgcatggtct
     1381 tcactggtta tgagcacgct catcaatgta cagtggcagt cggggctgtt cgatcatctt
     1441 ctcagactac cgctggcgtt ttttgaacgc cgaaaattag gtgatatcca gtcacgtttt
     1501 gactcccttg acacattgag ggccacattt accaccagtg tgatcgggtt cataatggac
     1561 agcattatgg ttgtcggtgt ttgtgtgatg atgctgttat acggaggata tctcacctgg
     1621 atagttctct gctttaccac aatttacatt tttattcgac tggtgacata cggcaattac
     1681 cgacagatat cagaagaatg tcttgtcagg gaggcccgtg ccgcctccta ttttatggaa
     1741 acattatatg gtattgccac ggtaaaaatc caggggatgg tcggaattcg gggggcacac
     1801 tggcttaata tgaaaataga tgcgataaat tcgggtatta agctaaccag gatggatttg
     1861 ctcttcggag gaataaatac ctttgttacc gcctgtgatc agattgtaat tttatggctg
     1921 ggagcaggcc ttgtgatcga taatcagatg acaataggaa tgtttgtagc gtttagttct
     1981 tttcgtgggc agttttcgga aagagttgcc tctctgacca gttttcttct tcagctaaga
     2041 ataatgagtc tgcacaatga gcgcattgca gatattgcat tacatgaaaa ggaggaaaag
     2101 aaacctgaaa ttgaaatcgt tgctgatatg gggccaatat ccctggaaac caatggttta
     2161 agctatcgtt atgacagtca gtcagcaccg atattcagtg ctctgagttt atctgtagct
     2221 ccgggggaaa gtgtggctat aactggtgct tccggtgcgg gaaaaaccac attaatgaaa
     2281 gtactatgtg gactatttga acctgatagc gggagggtac tgataaatgg tatagatata
     2341 cgccaaattg gaataaataa ttatcaccgg atgatagcct gtgttatgca ggatgaccgg
     2401 ctattttcag gctcaattcg tgaaaatatc tgtggttttg cagaggaaat ggatgaagag
     2461 tggatggtag aatgtgccag agcaagtcat attcatgatg ttataatgaa tatgccaatg
     2521 ggatatgaaa cattaatagg tgaacttggg gaaggtcttt ctggcggtca aaaacagcgt
     2581 atatttattg cacgagcctt ataccggaaa ccaggaatat tatttatgga tgaggcaacc
     2641 agtgctcttg attcagagag tgaacatttc gtgaatgttg ccataaaaaa catgaatatc
     2701 accagggtaa ttattgcaca cagagaaaca acgttgagaa ctgttgatag agttatttct
     2761 atttaaacca tagaggaatt acaagcgtat gaggaatatt tcttcctgtt ataattcctc
     2821 gttatgctca gatatctgtt ggaggtggaa tggaagatag acaatccacc aagaagaaat
     2881 atcattctgt gtggattgtc caataactgt tctttcttat attaaataat actatttata
     2941 aacaaacatc actaagatta tttggactcc aattacacaa tcttcccgca gcatagttcc
     3001 atgcttctga aggtatccct tcgggttttt gcttaattgt tccccctaaa ccggatggag
     3061 acattgcagg attaggtttg tgagtggatg catagtcata tattgcacct ccagccacac
     3121 ccccagcagc tgctccaatt cctcctgcaa caaattgccc ggatagtgtt cctatagcca
     3181 tcgcaatatc acgccctgaa gcaccaccag aaacagaatc taattcattt agagtcagag
     3241 ttctcatatg atctcctttt tatcttatcg gatattgaat aataattatc accaacaaag
     3301 taacatattg cagacattaa tgcagagaag caaaatgtat gcatggataa aaagtccttt
     3361 cctctaaaaa cacaatcata tatagctaat gcaatatata ttgcggtggc atttattata
     3421 aatgcaaata acaactctaa ttttgttctt tttctatcca ttacttttta tcccattact
     3481 ttctatccca ttaccacaca aacactaacg ataatgatta tcgttaacat agtcaagagt
     3541 gaagggtagg aggccctcaa ccccctataa ggggtccgct tggaaaacgg atttccccac
     3601 gtcaagagaa ttgatttgaa cgagtggctt cgctacaaac agctcccgtt tgtgtgtgaa
     3661 aaacccctct caacagatct caactctgca cgatatctac aggagattgc tccaaaaggc
     3721 agtatcgtgt cttttaacta ccgatataac cccgtcatcg acatgataat gcgtctgaga
     3781 aatgagaaga aattaggtga actgcatttc ttctcagcag aatttaataa aaattctgca
     3841 ctgacccgcc atcacctgac ctggcgagac tccgcacaac agagcaaaag cagcggtgct
     3901 ctgggcgatc tatcctgtca tctgcttgat ttattttgtt ttatagggga aagcccggta
     3961 gtggttcatg ggatcaaaac ggtaaagggg acacgtgggt acaaaatcag atggtcaggt
     4021 tgaagtggat gataacggct atgtaatggg cagttcagaa aagggagctt atttcagagt
     4081 tcatgccagt aaatcggaaa cagatcataa tttgggactg catatacagc ttgtatttga
     4141 aaatggtgaa atcagatatt caacccacca tgaaaatcgc ctgctcctga ttctatttaa
     4201 tgatacgaat acggaaacaa ttggcttcga cgctctgaaa cgcttgcctg atccaccacg
     4261 ggagcttcca ttctggtctg attcgtttat tcatcttcat gacgactggt gtgctcttat
     4321 taaatatggt cactcgtctc ctaagctggc ggacctgtca tccggccttc atatacagga
     4381 aataattgag gcattctgat atggatacca ttcttttaac cgggctgttt gctgcatttt
     4441 tcacaacgtt tgcgttcgct ccccagagca taaaaactat caggacacgc aataccgaag
     4501 gtatctccgt tgttatgtat atcatgttcc tgacaggtgt aatctcatgg atagcgtatg
     4561 gcattatgcg atctgatttt gccgtactga tcgccaatat tgtcacactg tttctggctg
     4621 caccagtgct tgtaataaca cttatcaacc gcaggaaaaa acacgtatga atattgcaat
     4681 catcggcgcg ggtcctgccg gcatcatttc tgcccgtaat gcgattaaag caggtcactc
     4741 cgtggttctg tttgagaaga atacccgaat tggggggatc tggaaccctt ggagtggtgg
     4801 cgcttaccgt aatgcctgta tgcagaactc tcgttataca tttcattata ctggctttcc
     4861 ccctggcgat atcgatgaat ttccaggagt ggaacaggta ttcagatatc tttcggccgt
     4921 ggccggagag gatgccctcc gtgagtcgat ccgactgaat actgaagttg tttcactcag
     4981 aaaagacgcc ggacaccggg tgatccgctg cgcttctgaa ggaaaagaca cggaagacat
     5041 ttttgaccgg gtcatcattg caacaggtga actctggcaa ccccgccggc cccccctgcc
     5101 aggtgaggaa aacttctccg gaacgttgat cacgtcgaga gattatcagg agccagaggc
     5161 atttaaagga aaaaatatcc tcatcattgg cggcggtgtc agcggtgcgg atattgcctc
     5221 agaccttgtt ccctttgcca gaagcgtaag tctgtccgtc aaaaagatgg gactttatct
     5281 gccgagacaa ttcccgactg gcccgaatga catgatgcac tcctatctgg gcaggtgtct
     5341 gctgagccaa atgaattatg aagattttat cggttatctc gacaccatga tgcctgacta
     5401 catgcaggcc taccgagcat ccagtctgtt gcccgacatg gcgaacaata acgcggtgca
     5461 tgttaacgaa aaaatcatac cgaatgtggc cgccggtctg ataaaagtta aaccccaggc
     5521 agagcgtttc accggagaag gcgctattaa gttcgtcgac gcatctcagg aaaaatatga
     5581 tgtaataata acctgtacag gctatgaaat gcctgattac tcgttcattc gtaagcgtac
     5641 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt
     5701 agtgggaacc aaagtgtcag atatgcagaa aaatgtgact cccggcaggc gaaaaggctg
     5761 ccctaattat cctcccgaat ttaaacagca gctcgttgct gcctcctgtg aacccgggat
     5821 atccatctca aaacttgctc ttgaaaatgg cattaacgcc aatctgttgt tcaaatggcg
     5881 acaacaatgg cgcgagggaa agctgctatt accttcttca gagagccccc agctacttcc
     5941 tgtgactctc gatgcagctg ccgaacagcc agaatcgctt gcagaggacc cggaaaccct
     6001 cagtatcagc tgtgaggtaa cgttccggca cgggacgctc cgcttcaatg gcaatgtcag
     6061 cgaaaagctc ctgactctgc tgatacagga actgaagcga tgatcccgtt accttccggg
     6121 accaaaattt ggctggttgc cggtatcacc gatatgagaa atggcttcaa cggcctggct
     6181 gcgaaagtac agacggcgct gaaagacgat cccatgtccg gccatgtttt cattttccgg
     6241 ggccgcagcg gcagtcaggt taaactgctg tggtccaccg gtgacgggct gtgcctcctg
     6301 accaaacggc tggagcgtgg gcgcttcgcc tggccgtcag cccgtgatgg caaagtgttc
     6361 cttacgcagg cgcagctggc gatgctgctg gaaggtatcg actggcgaca gcctaagcgg
     6421 ctgctgacct ccctgaccat gctgtaaatc tctttatcct ggttgtcaca gaataagccc
     6481 ggtaaaatac gggcttatga acgacatctc ttctgacgac atcttcctgc tgaaacagcg
     6541 cctggccgaa caggaagcgc tgatccacgc cctgcaggaa aagctgagca accgggagcg
     6601 cgaaatagac catctgcagg cgcagctgga taaactccgc cggatgaact tcggcagtcg
     6661 ttccgaaaaa gtctcccgcc gtatcgcaca aatggaagcc gatctgaacc ggcttcagaa
     6721 agagagcgat acgctgactg gtagggtgta tgacccggca gtacagcgtc cgttgcgtca
     6781 gacccgcacc cgtaagccgt tccctgaatc actaccccgt gacgaaaagc gactgttgcc
     6841 tgcggcgccg tgctgcccga actgcggcgg ttcactgagc tatctgggcg aggataccgc
     6901 cgaacagctg gagttgatgc gtagcgcctt ccgggttatc cggacggtac gggaaaaaca
     6961 tgctccatgc cgatgatacc ccggtccagg tactgctgcc gggtaataag aagacgaaga
     7021 ccgggcggtt gtgggcgtat gttcgtgatg accgcaatgc cgggtcagcg ttggcacctg
     7081 cagtgtggtt cgcttacagc ccggacagga aaggcatcca tccgcagact catcttgctt
     7141 gcttcagcgg tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg tatcgcaatg
     7201 gtcggataac ggaagctgcc tgctgggctc atgcccgccg aaagatccac gatgtgcacg
     7261 tccgcatccc gtcagcactg acggaagaag ccctggagca gatcggtcag ttgtacgcca
     7321 tagaggcgga tataagggga atgccggcag agcagcggct tgctgaacgt cagcgaaaaa
     7381 cgaaaccgct gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag accctgtcgc
     7441 gacactcaga gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt
     7501 actatgcgaa cgatggctgg gtggaaatcg ataacaacat cgctgaaaat gccctgcggg
     7561 cggtcagtct gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtggcgggg
     7621 agcgctactg tacagcctga tcgggacgtg caaactgaat gacgtggatc cagaaagcta
     7681 ccttcgccat gtgcttggcg tcatagcaga ctggccggtc aaccgggtca gcgaactgct
     7741 tccgtggcgc atagcactgc cagctgaata acacatcccc gtcaatacgg ccctcgctgt
     7801 acgcttacca ataaataata tgcttatcga cctgatggct gcaatgtcgc ataaagactg
     7861 gctgagtcgt cgtcaacgac aaaaacaggg aattgaacgt gcccatactt taggtaagta
     7921 tcggggcaaa caggcagatc gggaacgaca tcagaaagta atgtactacc ggcagatcaa
     7981 gaaactgagc atccgtgaaa cggcagaggt gacaggctat agtacttccc aagtttgtcg
     8041 cattcaggcg ctctataaag aggttaaacc tgattaaaaa atagccttaa cgtacaatat
     8101 tttccgtttc ccaaacggag cccaatacag atggctaaca gaccgtctgc ttccagttca
     8161 aatggtttac cgtcgcggtc cagggcgcga attttaactt tctctgcgcg tggcactaaa
     8221 gctcgttgtt cagggattga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct
     8281 aaaagctccg gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaattaca
     8341 atgatacgtt gatggatatc aacctgggtt gccgccaggc caatactttt tctgcgtaca
     8401 tcgtctcgaa catatcatcg acgatacgct gaatttctgc attcacttct tctaccggtt
     8461 tagcaacttt gcgaagccgc tcgtccggaa tatgtaacac ttgcaaaact gacataaatc
     8521 tccagagatg tgttcaggag ttagaaagat tatttcttct actgtaccgt aatcagataa
     8581 tcgcagcaaa gggacaaagc aatagtccct ttgctgcaca gcatcagaat gaataacgaa
     8641 tattagcgtt tatcgcatca tctgtgttgt atttaccaaa tgcagagcgt tcaacttcca
     8701 gccccagacg cgtattgtcg ccaaaccggg catttaaccc cacaccgtaa agcatacgac
     8761 tgtcttttct gccattaatc tgatgttctc ccgctgcatc cttcagatga acgtcagcac
     8821 tgtccgtcag atcgaactca taatgcaggc cggcacgggc tgtcagactc cagtccttac
     8881 cactgaaggt tttaccggaa acaacgccgg ttctgcctac cagaggatta acgctgttac
     8941 gacgcattga gacatccatt ccactgtcgt tccagttaaa tgtttggccc tgcagtcttc
     9001 cccagaccag ttccgcctga ggttcaacaa acgtcgtatc tgtcagatga taacggtatc
     9061 cgacttctgc acctgcatac agtgaatggc tgcggaagtt ctgtttacca gctccggcaa
     9121 agttcaggtc atatttgttt tcattgtgaa tatatttggc aatcacatca aagtaagcgc
     9181 cggaccggaa cagaccactg gcatagaaac caccacccca tgattttgtt ttaccgctgt
     9241 acaggtctgc tgacgcatct gtgtcagtgt aggtggccat cacgccggta aacaggtcca
     9301 tacttcccag ttcgtgctta cggtcagccc ccatctgcag cagggtatag tggtcagtga
     9361 aaccgccatc agcagagccg gaaccgttca gcagacgcac ccacgtaccg gcttcgccat
     9421 taatatccct caaatcgccc atgcgtttgt tcaggttgtt aacttcagtg atgaagttgt
     9481 tatagctgat gtgcatgaat gtggcggcag ccttaccctg gccgtcgtta cgtgcaacct
     9541 ggtaaccatc gaggacccac tcttttttcc cgtcctcttt tctgacacta aggatggggg
     9601 tgacatcact gaatcccaca acccgtgttg atgccctgaa cagattatca gctgtcgctt
     9661 caggtgcgct gaccagtgga atatcaagcg tgtccttgtt agaaggtttt ttcaggaagt
     9721 taacccagat gctgttgtca tgacctgttg ccgacttgtt tatcaccagt ttgtctgcct
     9781 tgttaaggtc tgtacgcatg acaaatgctg actgaaccgc gtccagatta tctgttgtca
     9841 gtgtcgtgaa cggtgatgtt cccccgttaa aaccgactat tgtccggtta agtttcatat
     9901 ttcctgccgt ggagtttccg ttcatcgacc actgggtgtc tgtcatgctg acggtggcat
     9961 ccggtgcatt caggctcccg ctccagatat tgcggtaccc gttaaacagg ctgtacaaca
    10021 tctgattctg aagagtcagg tcaggactca gttccccttc ccctccgagg gtgacagtcc
    10081 ctttatcctg aacattgata ttacctgaca acatactgta cggccccacg ttcaggcggg
    10141 catcgtctcc cttcaggttc catgaaccgg cataatcgta tacaggtaaa agtgtgtgag
    10201 atacctcatc aggacggctg ttcagactca gggtggcatc agaaatattc actggaccgt
    10261 cagaaacaaa actctgactg gccagagcat ttgctccctt gttcagattc agggcggtac
    10321 tggtcagtgt tgagttcccc agaacggcac tgtctgagga gatattcacg gtactgttgt
    10381 tcgcctgtat tccgccattg aatatatcat tgatattcag cactgactga ttatccaggt
    10441 tgacggtgcc gttgaagaca cttttatctg catctttagt tgcaacagat gtgccttctt
    10501 caagggtaaa ggctgttccc tggccatcgt ttttgtcgat aaatacccgg ctgtcgccca
    10561 gcgtgacgct ggagttatct gcctggatgg ttgtgttcag tgtggcattg cgaccaagac
    10621 caaaatcagt gttcttcagt gacagcctgt caaaggtaaa actgcggttc tcccagtcct
    10681 cctgactgaa tgacgtaggc tgagtcagaa ccgaatggtc tccactggca gccagtttgt
    10741 cagccacaga ctgagtattg tatgcatgga taaccggatg cccctgcagc gtcagacgcc
    10801 cgttttcctg ggtgaatgta ccggagatat ccgcagctcc gtccatcacc agagcactgg
    10861 taacgccttc aggcaggcga ttgtccacat tcagattgcc tttcagttgt ccgtgaaaca
    10921 gataccctgc agtattgaaa cggtcagcta ccagtttctg tgcatccccc tgactgtgcc
    10981 ccacaaactc ccaggtggcg ttgctgctct ggtccgtggg gaaataacca taggtgctct
    11041 gcttcaggat gaagtaatcc gtcgtatttg tgtacgggtt attgtattta tataaatttc
    11101 cggcagttcc tttacctgat tccgaccagc cattcagtgc tactttgtca gcccgcaggg
    11161 catagtccag cgtgatagtg gcccgtttat caacgttatt cgccagcacg gcaccataat
    11221 ctgccgcctt caactgatga aacgtcagac tgttaccatt aacatccagt gtgcccccac
    11281 gatatcccca tgagacggta tccggattta cctgccgctc atcagtcagt accacggtcg
    11341 gccggccact ggcaatatta acactgctga acgcctgcac ctgtccttta ttgtccgcct
    11401 gctggttcag tacaaccttt ccgtccccga ccttcaggcc accttcatta atacctgtac
    11461 cctgtaccgt cagcgtacct tcaccaattt tatgcaggtt atcgccctta acaccattaa
    11521 cctgccagtt tacggacacc ccgttgtcca caacaatacc ggcaccggtc caggtacttc
    11581 cgttagaggt tgttactgtg taattatcac ggaacgtcag ggaacccgcc ccctgagaaa
    11641 ccgaatcctt aaggttaatc tgaccattct gcccctgaaa tatcaggttc ttaccagcat
    11701 tcaggtcatt tccctgctgc ccgtgcatgg catatgtggt ggttccctgt gtcagcgcac
    11761 cagctccggt actgctgtta aagctccact ccagtgcacc accttccgat gttctgaacg
    11821 tgaccggggc atcattgtct tcattaaatt tctgcccgat aaaatccagt ggaataacag
    11881 cccagttatt tcccctgccc cccgcgccat tccccgcagt aagaacaccg accagcaccc
    11941 atttattctg aacagtatca aaagcaaata aaggcgaacc gctgtcaccg gcctcgccat
    12001 aaatgggcat tgcaccatta agtttgtaat caaaaaccag acctgaactg gtgctaatca
    12061 tttctccatt ctgataggat gacaggctac cgacagtccc gccggtcagc caggaatatg
    12121 cacctcccat ttttgtcagc tgtccgttac tgtccttaat atactgagta ccagacccca
    12181 gacgataaaa aacaggatag cgctccttat ccagatatgc gccagccact gccccctgcg
    12241 ccgtcaccgc agtaggggca acctctgtca ccagtttatc cagccggggg gcatgaaaat
    12301 ccagtgacgg cgcattattc cggtccacga tattgtaacg gttttcacca tcaccaaagc
    12361 taacgtttgt atatcccccg ttatgtttca cgctggcgat atactgcggg tttatcagtg
    12421 tcgccacacc gatttccgaa tccacagcac tgaaatcagg catagctgcc ttatccagcg
    12481 taccgacaaa ttctccctgc ttattataaa tagcgatatt cgttgccccc gggcggaaca
    12541 tccccttatt ttcagcaaaa tcacgaaata actgataccc gagttcatta ttgaccgttc
    12601 ccgcaagact tcctgcagag aatagtaccg ggatcagtaa taaaaccggg aaacacagac
    12661 gtctgacaga cttatgaaca catttcctag caaactcaga tacggcaata aagccccggg
    12721 ccacagcgct gtagcgaaga gaataaattc tgttcatttt taattactcc tgagaaaaat
    12781 aaaatcagta ctactcaggc ataacggata atcaccatca tcctgaggaa tccccagaaa
    12841 aaggagacag gcatagcgtg atgtgaacta ctgattcttt cacaaattca gggagagcac
    12901 cgttatgcct gcacataaag tatgccagaa ccggtggcga atgtgtgcta tgcacaaatg
    12961 gtgaaacagt tcctgagtcg tgacccgctc gaatgcgtgc tgtgtggcgg ccggatggta
    13021 taccggcgtg ccatagcggg gctgaatgtg gacgggctga agaaaaacgc gcgggatatc
    13081 agtctgataa ggattacttt ccagccggct gatggctgac gggcgtaatc ccattcgttc
    13141 ggccagcgca gactttgtta accccgcttt ttctctcatt tcgtataaca tttctaccat
    13201 ctccagttct ttgtcagcat cttcataccc tttaacagct tctggagtat taagcagttt
    13261 ttgctttacc tggctgaacg gaacactttt tactttcgtc atttcatctc cttcaggcgc
    13321 atccttgaag tggtttactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga
    13381 acaacacagg tgctccaatg aaaaaaagaa atttcagcgc agagtttaaa cgcgaatccg
    13441 ctcaactggt tgttgaccag aactacacgg tggcagatgc cgccaaagct atggatgttg
    13501 gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac
    13561 caaaagcctc tccgataaca ccagaacaaa tcgaaatacg tgagctgagg aaaaagctac
    13621 aacgcattga aatggagaat gaaatattaa aaaaggctac tgtagattca attggtcaac
    13681 gcaacagtta tgtgaaaaca tggggttgcg gaggtttttt gaatgagacg aacatttaca
    13741 gcagaggaaa aagcctctgt ttttgaacta tggaagaacg gaacaggctt cagtgaaata
    13801 gcgaatatcc tgggttcaaa acccggaacg atcttcacta tgttaaggga tactggcggc
    13861 ataaaacccc atgagcgtaa gcgggctgta gctcacctga cactgtctga gcgcgaggag
    13921 atacgagctg gtttgtcagc caaaatgagc attcgtgcga tagctactgc gctgaatcgc
    13981 agtccttcga cgatctcacg tgaagttcag cgtaatcggg gcagacgcta ttacaaagct
    14041 gttgatgcta ataaccgagc caacagaatg gcgaaaaggc caaaaccgtg cttactggat
    14101 caaaatttac cattgcgaaa gcttgttctg gaaaagctgg agatgaaatg gtctccagag
    14161 caaatatcag gatggttaag gcgaacaaaa ccacgtcaaa aaacgctgcg aatatcacct
    14221 gagacaattt ataaaacgct gtactttcgt agccgtgaag cgctacacca cctgaatata
    14281 cagcatctgc gacggtcgca tagccttcgc catggcaggc gtcatacccg caaaggcgaa
    14341 agaggtacga ttaacatagt gaacggaaca ccaattcacg aacgttcccg aaatatcgat
    14401 aacagacgct ctctgggaca ttgggagggc gatttagtct caggtacaaa aaactctcat
    14461 atagccacac ttgtagaccg aaaatcacgt tatacgatca tccttagact caggggcaaa
    14521 gattctgtct cagtaaatca ggctcttacc gacaaattcc tgagtttacc gtcagaactc
    14581 agaaaatcac tgacatggga cagaggaatg gaactggtca gacatctaga atttactgtc
    14641 agcgccggcg ttaaagttta cttctgcgat cctcagagtc cttggcagcg gggaacaaat
    14701 gagaacacaa atgggctaat tcggcagtac ttgaaccgcc ccggaaatcc tggagactaa
    14761 actccctgag aaagaggtaa acaggatgac taaaaatact cgtttttccc ccgaagtccg
    14821 tcagcgggcg attcgtatgg ttctggaaag tcaggatgaa tatgactcac agtgggcggc
    14881 aatttgttcc attgctccaa agattggctg tacgccggag actctgcgtg tctgggttcg
    14941 ccagcatgag cgggataccg ggggcggtga tggtgggctc accagcgctg aacgtcagcg
    15001 tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agtaacgata tccttcgcca
    15061 ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaaat gatgccactg
    15121 ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagcgaact gcatattgcc
    15181 ccgtcaacgt attaccattg tcagcaacag cgacatcatc cggataaacg cagtgcccgt
    15241 gcgcagcacg acgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag
    15301 gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag ggtggccaga
    15361 tgtacagtgg cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag
    15421 gtccgtacga ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc
    15481 gtggcagaac gacctgacca gctgtgggtg gctgatttta cttacgtcag cacatggcag
    15541 ggcttcgtct atgtggcgtt tatcattgat gtgtttgccg gatacatcgt ggggtggcgg
    15601 gtctcatcgt ctatggaaac gacattcgtg ctggatgcgc tggagcaggc gttgtgggcc
    15661 cgtcgtccgt ctggcaccat ccatcacagc gataaaggct ctcagtatgt gtcactggcc
    15721 tatacggagc gactaaaaga agccggatta ctggcatcaa cagggagtac aggcgactcg
    15781 tatgacaacg cgatggctga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
    15841 aagagctgga aaaaccgtgc agaagtggaa ctggccatac taacgtgggt ggactggtat
    15901 aacaatcgac gattgctggg aaggctgggc catactcctc cggcagaagc agaaaaagct
    15961 tattatgctt ccatcggaaa cgatgatctg gcagcctgag ttcacagata aaacactctc
    16021 caggaaaccc ggggcggttc aactttccta aaaagacatg tcttgcccaa tatactcaac
    16081 atgaactaga tctggttgct gctcagctaa acaacagacc gagaaagaca ctgaagttca
    16141 aaacaccgaa agagataatt gaaaggggtg ttgcattgac agattgaatc tacaaccgcg
    16201 ctcttgatgt cagactccct cgagtaggca gcctggcggc tgcggcttgt catggcctgg
    16261 aattaccgtt ataaaaaaag ataatgtcat tgtctttcag gtagttatat ggcccgttca
    16321 gctaaacccc gtaaacgcaa acccgcacca caaagaagca aacttccccg ctatgttgtg
    16381 aaacttcatc cggatgattt ttttgacgaa gaagacgctg aagttctgcg ctttgataat
    16441 tttgacgatg ccgttgagtg ctgcgctgac ctgggtattc cgttctttct ggatgcagga
    16501 aacaaaaagc tggtcttctg gtttgttcgt gtcgatgacg aagggtatcc ggaaatagcc
    16561 cgctgtacgg agcgggagtt tgcaaccatt cttgccggta tcagtgccgg tggtatgtac
    16621 tgcccggaat gcggcacagt tcactggccg gatggcgtta ccccacccgt ctgatgcttc
    16681 cccgttttgc cgatattttt cagcagggta accgctggct taactggctg gagaaacagc
    16741 cggaaggttc agtgcgtccg gtggtgactg agtcagtgac aaaaatcatg gcatgcggga
    16801 ccacgctgat gggctacacg caatggtgct gttcgtcacc ggactgttgc cacaccaaaa
    16861 agatctgctt ccggtgtaaa gccgctcctg tccgaactgc ggggtgaagg ctggcgcaca
    16921 gtggatacag tatcttgtat gtaataggac ttgatggcta gctaccatca tatgaagcag
    16981 agtgagcttg tcccagccct gaacagtcag actttgatta gcagcaaaag ccctctgctt
    17041 cacctttcac gccagcatta acattgaacg gttgccaaga gcgatgactc tgcatgtaca
    17101 aggggaatag gcgtgatggt ttggtgaaag gagaatattc atgtttcagt taggtattga
    17161 tgtcagtaag aaaacgctgg atttgtgcct gctgcgcgaa ggcgtaaaag gcagcgttaa
    17221 aacccgaaag cttcagaatg atattaatgc tgtcaacgcg gtaatagcct ggctcagtaa
    17281 gcaacattgt aaacctgaag atgtccatat catcatggaa gccactggtg tttatcatga
    17341 atcactggct tacgggcttc acaaagccgg gggccggata tcacttgcca acccacacag
    17401 ggcaagagag tttgcccggg gtatgggaat gctgaccaaa aatgaccggg ttgatgctta
    17461 tatgctggct tgctatggcg cgcttaaagc accggagcca tggcttcctc caccggaaga
    17521 agtccgccat ctcagtgcac tgctccgcag acgtgatgct cttgtggctg acagcactcg
    17581 cgaaaaaaaa caggctggaa aaatacctgg caacagacac accttctgtt atagctgaat
    17641 ccgtagaggc aatgattggt cagttgaacg cagaaattaa acgtctggac agactgatcc
    17701 gttcacacct taaccagcat cctgaattaa aaaaggattt tgacctgctg acgtcaataa
    17761 aatccgtagg ttatcagtta ggtctgaata tgcttgtcat cctgcggagc catgatttcg
    17821 ccacagcaga acaggcggct gcatttttag gtgtcgttcc cgttgaaaaa tattccggca
    17881 cctcggtcag agggtgtccg aaactgtcaa aaattggtcc gccggaaatc cgggcgaaac
    17941 tgtatctggc ttcgttgtgt ggtctgagat ttaacccatt gatgaaagcg atgtatgagc
    18001 gattatgcct gagaggtaag ggcaaaatgt gtgccattgg tgcgctgatg agaaaactcg
    18061 tgcactggtg ctatggcgta ctgaagaccc aaaaaccgtt tgatgtgggc tatttgtctt
    18121 catatgcgcg tagcgcatgt aaggcggcat gatgtagtta catatggtac aggagtaaca
    18181 ccgccgaagc ccggcgtaag ccggtacgta ttgataggtt tcacctatcc catccctgcc
    18241 ctgcgaggcc atgcgggctt tcagcgatga tgaagtacag gtaagccctt ttaaccccgt
    18301 tagcgcggct acgccgcgct gtatcgggga cttagtgctg acagcttgct ggcagtactt
    18361 agggggctgt acgtcggagt tcaagcgatt tttcttgaca gaacacggta gctgctaagc
    18421 catacccgct ttcagccatg agagagcttc tgtgcgcggt cggagtggtc ccgacgaggg
    18481 tttacccgaa gtcgggacgt atctccgcgt tagcggggtc agtttggaat tcggaaaata
    18541 aaccatgtta agactatttt ttagccaaag ggctttcagg aatcgccaga atggcgggac
    18601 aacagtggtt ttggtgtccg aaatcgaccg gtttcggaca ttgttaaaca gatatatccg
    18661 aaagtcagcg aaattacttt cggacatgca ttataatatg tacacccatt tcgtacagaa
    18721 gatctcacta tgtctcgaat ttttgcctat tgtcgggttt ccactctgga acagacgacc
    18781 gaaaatcaac gtcgggaaat cgaaagcgcg ggttttaacg tcagacccca acgaataatc
    18841 gaagaacaaa ttagtggatc agtagcagcc agtgaacgtc caggttttaa ccggttgctt
    18901 gatcgtctgg aaagtggcga tgtattgata gtcacaaaac tggatcgcct cggtcgtaat
    18961 gcaatggata tcaggaaaac agtggagcaa ctgaccgaat ctggcatcag ggtgcattgc
    19021 ctggcattag gtggtgttga tctgaccagt tcggcaggaa aaatgacaat gcaagtgatt
    19081 tcagcggtcg ctgaatttga acgagatctt ttacttgaac gaacttattc agggatagca
    19141 agagcacggg atgcaggaaa acgttttggt cgcccaccgc tattaaatga agaacaaaaa
    19201 cagaaggtac tcgaacgcat taagtctggg gcaagtataa gtgctattgc cagggaattc
    19261 cgaaccacac ggcaaacaat tctgagagtt agagcgaaac agcaaactgt tactgtatag
    19321 aaaatagtct tagcgtggga tattttccgt tttccaagcg gaccccaagg aagactacat
    19381 cgcgttcatg ccgaaaccga atgtaagaac ggcattgcat aatcttgcag tggcgatcga
    19441 acattacaat gaaaaccatc cgcacagtgc gttgggttat cgctctccgc gagaatatcg
    19501 acgtcagcgg gtaacattaa cttaagatac aacccctgtc tggaaataag ggggcaagaa
    19561 cacattgaca ctataaatat ggcattgtat aaaaccccgc gataataatg agtttcattt
    19621 gcattactcc catttgctgg tcatccactc gcctgtttac aaggatagcg tttaatgaag
    19681 aaggtccttt gcgcgttagg cttgatgttc accgccgtca gttccgcgct ggcaacaacc
    19741 taccccctga cgattgaaaa ctgcggctat caggagacct tcacccggcc gccggagcgc
    19801 gtggtcgcgc tggggcaaaa tacggtcgaa attctgctgc tgctcgggct gcaaaagcag
    19861 gtggtcgcca gcgccttctg gccgaccagc gttttaccgc agctggctga gcaaaacgcg
    19921 aagattaaaa ccttgacggt ggaaattccg agcctggaat cggtgctggc gcaaaatcct
    19981 gatttcgttc ccgcccagtt gccgctgctg ctggggccgg aaagtaaagt cgctaggcgt
    20041 gaagatctcg ccaccgtcgg cgtgaacagc tacgtctcac cgggggtgtg cgccaccaaa
    20101 aaggccaccg gcgatatgta cggcagccgg caaaagctgt gggatatgac ctggctgtat
    20161 caggaaatta gcgatttttc ccgcattttc aacgtcgaag atcgtggtca ggcgctgatt
    20221 gccgatttca aaaaacgtga agccgacctg cgccaggagt ttggtaaaag caaaaaggat
    20281 ctctccttcg tcttctggtt ctccagcgcc tcaccttcgg ccgatgccta tgtcggcggc
    20341 aaaaacagcg cctccggatt tatcgccagc gtgctgggcg gacataatgc gattacctcc
    20401 gaaaccgagt ggcccacggt gagctgggag agcattattg ccgccaaccc ggatgtgatc
    20461 gtggtcgcca gcctagatcg taaccgctgg gcgctggata aagccgagga gaagatcaaa
    20521 ttcctcaaaa gcgatcccgc cgtcagccag ctggaggcgg tgaaaaaagg ccatatcgtg
    20581 gtcatggacg gtcaggcgat gaatccgacc attcgcacgc tttacggcgc ggagcaggtg
    20641 ggcgagcagc tgagaaaaat ggggctgaac tgatgagcgt cggcgcgatt gagacgagcc
    20701 gcagcatact gctcaccggc tggtgtgtac tggcagcgat cgtgctggcg ctggtgatcg
    20761 ctgtgggcgt cagcgttggc gagctggcca tcccgctgca gaatgtcttt tatgctatca
    20821 gcaacagaac cggcttaacc gctgagccgc tcaaccgtat ctatgagagc gttatctggg
    20881 atttccgcct cagccgcgcg ctggtcgccg cctgctgcgg cgccggcctc gctatctgcg
    20941 gtgtcgtgct gcaaagcttg ctgaagaatg ccctcgccga accctacgtc ctcggcgtct
    21001 ccgccggggc ctccaccggc gcggtgtcga tcgtggtgct gggccttggc tctggcgcaa
    21061 tttccctctc ggcaggcgcc tttgccgggg ccttcgccgc cttcgccttt gtcgccttgc
    21121 tgactaacgg cgcgcgcggc ggcaatgagc gtacgattct tgcgggcgtc gccgcctcgc
    21181 agttatttaa cgccatcacg gcctatacca tcagcaccac cgccagcgcg cagcaggcgc
    21241 gcgatgtgat gttctggctg ctgggcagct tcagcggcgt ccgctggccc gaattccagt
    21301 tggtcatcgt ggtggtgctc gcgggtctgg cggtctgcct gtggtatgcc cgggcgctgg
    21361 acgcgttcac cttcggcgac gatgccgccg cctcgctggg gattgctgtg ccgcgcgtgc
    21421 gcctgatcct cttcaccact gcggcgctga tcaccgccac catcgtcagc atggccggct
    21481 caatcggctt tgttggcctg gtggtgccgc acgtgatgcg tttcttcttt ggcccgctgc
    21541 accgaacgtt gctgatcgcc agcgcgctgg caggggcgat attgatggta ctggcggaca
    21601 ttgcgtcgcg cctgctgatt gccccgcaaa gtctgcccgt tggggtagta accgccctgg
    21661 ttggggtgcc tttctttgcc gtgattatct accgctcaag gaataagtga tgagtatttg
    21721 cgctgaaaat atcacctgga aggcaggcaa aaaggtcatc gtcaataatg tctcgctgcg
    21781 ggtgccgcgc ggagaaacgg tcggactgct ggggcccaac ggctgcggca aatcctcgct
    21841 gctgcgcgtt ctggcgggcc tgcgccgccc ggatgcaggt cgcgtcaccc tcgacggcca
    21901 ggatatcgcc cggatggcga aaaagcagct cgcccgccgc gtggcttgcg tcgagcaaca
    21961 cggcatgacc gaggccaata tgcgggtgcg cgacgtcgtg cgcctgggac gcattcccca
    22021 ccactctccg ttctcaaact ggagcgctca ggatgacgaa gcgattgccg ccgcgctgca
    22081 gcgggtagcg atgctggaga aaagcgaaca gggatggtta agcctctccg gcggcgagcg
    22141 gcagcgggtg catatcgccc gcgcgctggc gcagagcccg agcgaaatcc tgctggatga
    22201 gccgaccaac catctggata tacaccatca gatgcagtta atgcagttga tcagcgagct
    22261 gccggtaacc agcattgtgg ccattcacga tcttaaccat gccgcgatgt tctgcgattc
    22321 gctgatcgtg atgcagcagg ggcagatcct cgccagcggg acgccggagg agattttgtc
    22381 cgaagcgctg ctgtgggacg ttttccgggt gaaaaccaaa atcgagatct ccccttacca
    22441 cggcaaaaag cacattcatt tcatcgttta ggttgggcgg atatgcctct tttctccctg
    22501 cgccctgcgg caaccctctg gcctccggtg ttactgggaa gccagttcgt ttttaatatt
    22561 ggcttttacg cggtcgtacc tttcctcgcc ctcttcctgc gcgacgacat gctgctctca
    22621 ggcggactta tcggcctgat cctcgggctg cgtaccttct cccaacaggg gatgtttatc
    22681 ctcggcggga cgctggccga ccgctatggc gctaaggcga tcattctcgc cggctgcgtc
    22741 gttcgcgtcg ccggttttct cctgctggcg tgcggggcgt cgctgtggcc cattatcctt
    22801 ggcgcctgcc tgaccggcgt cggcggcgcc ctgttctccc cgtcgataga ggccctgctg
    22861 gcgcgggccg gtacccacag ccaggccaac gggaaacgca gccgcgccga gtggtttgcg
    22921 ctgtttgcgg tatgcggtga actgggagcg gtgatcggcc cggtggccgg aggcgtgctg
    22981 agcgggatcg gctttcgaca tatcgccctc gccggcgcgg gaatatttct tctggcgctg
    23041 gcggtgctct ttttcggtct gcccgccgac gggcacacga caacaacgcg cagacgagtc
    23101 ccatggtgga cgcctttgcg ccagccgcgc tttgtcgcgt ttattctcgc ctacagctca
    23161 tggctgttga gctacaacca actctacctc gctctgccgg tggagatcca acgttccggc
    23221 ggccgcgagc aggatctggc gccgctcttt atgctggcct cgctgttgat tatcaccctc
    23281 cagctccctc tcgcccgctt cgcccggcga atgggcgccg tgcgcatcct gccggtgggc
    23341 tttctgctgc tgtcggcttc ctttgccagc gtggcgttat tcgccgccgc cccacccgcc
    23401 gaaagttggc tgcggttaat gccggcggct ggttttgtca cgctgctcac cctcggccag
    23461 atgctgttag tgcccgcagc caaggatctg atcccgctgt tcgccgagga gtcgacgctg
    23521 ggcgcccact acggagcgct cgccaccgca ggagggtgcg cggtgttagc cgggaattta
    23581 ctgctcggtc atttacttga ccaggcgctc attccgtcgc cgcaggcggt ttatccctgg
    23641 cttctgctgg cgctgtttcc gctgtgcagc gccgtcgccc tgcgggcgat ctgtcgcccg
    23701 ctggcggcaa catgacgcag agcgggtaac agcccgctct ggaagtgaac agctggtatc
    23761 catattttca gctaatcctt taacaaagat cctcagccct gtttaaagcc cggcttgccg
    23821 tttttggctc gccattcctt cacttctttt acgatacctt caggactgtc ctcacggtca
    23881 tcccttggat aataaataag atcagaacca tcagggtgtt cagctaatcg acgaaattca
    23941 tccactaatt tatcatcatc agcctcagtt tcaccttcag cccggcagat ttttctgacg
    24001 aactccagaa actccgcctc ggtatagtca gaaatcgttt tcttttccat ttacttattt
    24061 cctctgtgaa tttcaatatg atgcttgggt gtcactaccc tgagattatc gatatcataa
    24121 acagcgccac catccgtaac gcgcttgata tggtgcagtt cgaaacgttc tcgttcacct
    24181 acacgctctg attcaggaac aaacgaagat ttaccttttg taaccagaac cttattctga
    24241 tttttgaact gcttcagtaa ctccggatct ttccccactt ccatccacaa cgccttacgg
    24301 aaccacggca gcctggaggc tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa
    24361 ccatccagaa tgagttccca tgtcacacaa cctagagcac cagaaagtcc acaccagaat
    24421 ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttatc agtccgtatt
    24481 tacagacttt atcgccggtc atccctcctg cacggtctgt ttctgggaaa ccttcaacaa
    24541 aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc gtcgtttgat
    24601 ttatacgaaa cagaagcgga gatgaaggcg gatgacccga agtggtggtg attttcagcc
    24661 ccgaccgctg aaacgcctgt tcacaatgtc gcggtataaa aatcgaccat gcagagagag
    24721 aaaccaccgg agtggtggga aaagcagaaa ggctcacgtg gcataaggcg tggacagcgg
    24781 tatcgtctgg tttttcaatt tcctatacgt gaacacaatc tgcaattcgg tatacagcga
    24841 acctgccacc ataagtgttt ttataccact taactggctc agcaccgggg gcaggcttca
    24901 ttctgaattc atcgcgcaga cgtcctttcg agaaacacct gtcttcggca atctcctgcg
    24961 cacgtctggt gaccagctca tcattattca tggttcttgc atctccgtca gacgttttta
    25021 tatccggaca gcccctctat ggccacttct gtgcagtatg caatacacgc aggatataca
    25081 cttttcccca ctggctgtcg acctcataaa ccagaacaaa atgcgggtga ataaccagtt
    25141 cccgggtacc ttccacacga ccattgcggc ctttatacgg ctgcccggct aggctgctgg
    25201 ctgaacaccc gcaaggttca cactgatgac acaccagtaa aagtactggc accgggcagg
    25261 aagaaggcga aaacaggata tatctggacg tatgtccggg atgaccggaa tgccggttcg
    25321 ccagaacctc cggcggtctg gttcgcctac tcaccggacc atcagggtaa acatccggag
    25381 caacaccttc gtcccttccg gggtatcctg caggcagatg cgttcgcagg ttacgatcgg
    25441 ctgttcagtg ccgaacgtga aggcggcgcg ttgacggaag caggatgctg ggctcatgcg
    25501 cggcgcaaaa tccacgatgt atatatcagt accaaaagcg cgacggcgga agaagcactg
    25561 aaactaatcg gcgaactgta cgccattgag cacgaaatac gcgggttgcc ggtgtctgaa
    25621 cgcctggcgg tcaggcaaat gcagagtaaa ccgctactga cttccttgta taagctgatg
    25681 caggagaaag aacacacgtt atcgaaaaaa tgccgtctga gagatgcgtt ccggtatatc
    25741 aggaagcact gggttgcgtt gtgcaacttc tgtgatgacg gtctggcgga ggcggacaat
    25801 aacacagcgg aaagagcgct tcgtgcagtc tgtctcggaa agaaaaatta cgtgttcttc
    25861 ggtagcgatc acggcggcga gcgtggtgca ctgctgtacg ggctgatcgg cacctgccgt
    25921 ctgaacggta tcgatccgga agcgtatctg cgccatattc tgagcgtact gccggaatgg
    25981 ctttccaacc gagttgacga actcctgcca tggaacgtag tactcaccaa tatgaaccgc
    26041 cccgggtttc ctggagagtg ttttatctgt gaactcaggc tgccagatca tcgtttccga
    26101 tggaagcata ataagctttt tctgcttctg ccggaggagt atggcccagc ctttccagca
    26161 atcgtcgatt gttataccag tccacccacg tgagtgtggc cagctccact tctgtccggt
    26221 ttttccagct cttacggtgt attacctccg ctttgtaaag gccattgatg ctctccgcca
    26281 tcgcgttgtc atacgagtca ccagtacttc ctgttgatgc cagtaatccg gcttccttaa
    26341 gccgctgtgt gtaggccagc gatacatact gagaaccttt atcactgtga tggactgtgc
    26401 cggacggtcg acgggcccat aacgcctgct ccagagcatc cagcacgaat gtcgtttcca
    26461 tagacgatgc ggctcagttc acgattttca cgttccagct ctttcagacg ctgacgttca
    26521 gcggtggtga gcccgccatc accgcctccc gtatcccgct catgctggcg tacccagaca
    26581 cgcagagtct tcggtgtaca gccaatcttt ggggcaatgg aacaaattgc cgcccactgt
    26641 gagtcatatt cgccctgact ttccagaacc atacgaatcg ccctctgacg gacttcgggg
    26701 gaaaaacgag tatttttagt cattctgttt acctctttct cagggagttt agtctccagg
    26761 attcccgggg cggttcagta ataccggtga tctcgaggcc cgtttgcaga agaacgggag
    26821 tgttcgtttc cgtgaccgtc ggacgggaga gtttgtctgt acggattatg gtgacagggt
    26881 tgttttccgt aatcaccatg accgcaatgc gctggcagat aaactggatt tgattgcacc
    26941 tgtattgttt gagcgtgatc ccagaatggg ctttgaacca gaaggaaacg acaaacaatt
    27001 taaccaggtc tttgctgaaa tggttgcctg gcataatgtg acaggccgta ccggtcacga
    27061 agactacagg attacacgac cggatgtgga tcatcaccgg gaaggaagtg aacgctacta
    27121 ccgcgattat atcgcggcga acagcaacga tgacgcatca ctgcctccgc cggagcagga
    27181 taaacgctgg gaaccgccat ctcccgtatg acctgtgtcc cttagcgaaa atctcgtagc
    27241 ttctctctca atgagggcgg gtatttgaca tcctccacgc cctaaggacg tgggttcctg
    27301 cttcaacggg cggcatccgt catccgccaa tacattgaat agcagcagac acctggttag
    27361 g
//
LOCUS       DAMZII010000061        27309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZII010000061 DAMZII010000000
VERSION     DAMZII010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.061"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..561
                     /gene="tuf"
                     /locus_tag="POA77_002485"
     CDS             <1..561
                     /gene="tuf"
                     /locus_tag="POA77_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6365893.1"
                     /translation="KPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQ
                     KSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY
                     ILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIA
                     MDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            744..938
                     /gene="bfd"
                     /locus_tag="POA77_002486"
     CDS             744..938
                     /gene="bfd"
                     /locus_tag="POA77_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDI6365894.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1011..1487
                     /gene="bfr"
                     /locus_tag="POA77_002487"
     CDS             1011..1487
                     /gene="bfr"
                     /locus_tag="POA77_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDI6365895.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1484..1951)
                     /locus_tag="POA77_002488"
     CDS             complement(1484..1951)
                     /locus_tag="POA77_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6365896.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWPFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2330..2641
                     /gene="rpsJ"
                     /locus_tag="POA77_002489"
     CDS             2330..2641
                     /gene="rpsJ"
                     /locus_tag="POA77_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI6365897.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2674..3303
                     /gene="rplC"
                     /locus_tag="POA77_002490"
     CDS             2674..3303
                     /gene="rplC"
                     /locus_tag="POA77_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI6365898.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3314..3919
                     /gene="rplD"
                     /locus_tag="POA77_002491"
     CDS             3314..3919
                     /gene="rplD"
                     /locus_tag="POA77_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI6365899.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3916..4218
                     /gene="rplW"
                     /locus_tag="POA77_002492"
     CDS             3916..4218
                     /gene="rplW"
                     /locus_tag="POA77_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI6365900.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4236..5057
                     /gene="rplB"
                     /locus_tag="POA77_002493"
     CDS             4236..5057
                     /gene="rplB"
                     /locus_tag="POA77_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI6365901.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5074..5352
                     /gene="rpsS"
                     /locus_tag="POA77_002494"
     CDS             5074..5352
                     /gene="rpsS"
                     /locus_tag="POA77_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI6365902.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5367..5699
                     /gene="rplV"
                     /locus_tag="POA77_002495"
     CDS             5367..5699
                     /gene="rplV"
                     /locus_tag="POA77_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI6365903.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5717..6418
                     /gene="rpsC"
                     /locus_tag="POA77_002496"
     CDS             5717..6418
                     /gene="rpsC"
                     /locus_tag="POA77_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI6365904.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6431..6841
                     /gene="rplP"
                     /locus_tag="POA77_002497"
     CDS             6431..6841
                     /gene="rplP"
                     /locus_tag="POA77_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI6365905.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6841..7032
                     /gene="rpmC"
                     /locus_tag="POA77_002498"
     CDS             6841..7032
                     /gene="rpmC"
                     /locus_tag="POA77_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI6365906.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7032..7286
                     /gene="rpsQ"
                     /locus_tag="POA77_002499"
     CDS             7032..7286
                     /gene="rpsQ"
                     /locus_tag="POA77_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI6365907.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7451..7822
                     /gene="rplN"
                     /locus_tag="POA77_002500"
     CDS             7451..7822
                     /gene="rplN"
                     /locus_tag="POA77_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI6365908.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7833..8147
                     /gene="rplX"
                     /locus_tag="POA77_002501"
     CDS             7833..8147
                     /gene="rplX"
                     /locus_tag="POA77_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI6365909.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8162..8701
                     /gene="rplE"
                     /locus_tag="POA77_002502"
     CDS             8162..8701
                     /gene="rplE"
                     /locus_tag="POA77_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI6365910.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8716..9021
                     /gene="rpsN"
                     /locus_tag="POA77_002503"
     CDS             8716..9021
                     /gene="rpsN"
                     /locus_tag="POA77_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI6365911.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEERWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9055..9447
                     /gene="rpsH"
                     /locus_tag="POA77_002504"
     CDS             9055..9447
                     /gene="rpsH"
                     /locus_tag="POA77_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI6365912.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9460..9993
                     /gene="rplF"
                     /locus_tag="POA77_002505"
     CDS             9460..9993
                     /gene="rplF"
                     /locus_tag="POA77_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI6365913.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10003..10356
                     /gene="rplR"
                     /locus_tag="POA77_002506"
     CDS             10003..10356
                     /gene="rplR"
                     /locus_tag="POA77_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI6365914.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10371..10874
                     /gene="rpsE"
                     /locus_tag="POA77_002507"
     CDS             10371..10874
                     /gene="rpsE"
                     /locus_tag="POA77_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI6365915.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10878..11057
                     /gene="rpmD"
                     /locus_tag="POA77_002508"
     CDS             10878..11057
                     /gene="rpmD"
                     /locus_tag="POA77_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI6365916.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11061..11495
                     /gene="rplO"
                     /locus_tag="POA77_002509"
     CDS             11061..11495
                     /gene="rplO"
                     /locus_tag="POA77_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI6365917.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11503..12834
                     /gene="secY"
                     /locus_tag="POA77_002510"
     CDS             11503..12834
                     /gene="secY"
                     /locus_tag="POA77_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI6365918.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12866..12982
                     /gene="rpmJ"
                     /locus_tag="POA77_002511"
     CDS             12866..12982
                     /gene="rpmJ"
                     /locus_tag="POA77_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI6365919.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13129..13485
                     /gene="rpsM"
                     /locus_tag="POA77_002512"
     CDS             13129..13485
                     /gene="rpsM"
                     /locus_tag="POA77_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI6365920.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13502..13891
                     /gene="rpsK"
                     /locus_tag="POA77_002513"
     CDS             13502..13891
                     /gene="rpsK"
                     /locus_tag="POA77_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI6365921.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13925..14545
                     /gene="rpsD"
                     /locus_tag="POA77_002514"
     CDS             13925..14545
                     /gene="rpsD"
                     /locus_tag="POA77_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI6365922.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14571..15560
                     /gene="rpoA"
                     /locus_tag="POA77_002515"
     CDS             14571..15560
                     /gene="rpoA"
                     /locus_tag="POA77_002515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI6365923.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15601..15984
                     /gene="rplQ"
                     /locus_tag="POA77_002516"
     CDS             15601..15984
                     /gene="rplQ"
                     /locus_tag="POA77_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI6365924.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16091..16459
                     /locus_tag="POA77_002517"
     CDS             16091..16459
                     /locus_tag="POA77_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDI6365925.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16470..16895
                     /gene="zntR"
                     /locus_tag="POA77_002518"
     CDS             16470..16895
                     /gene="zntR"
                     /locus_tag="POA77_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDI6365926.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16951..17169
                     /gene="arfA"
                     /locus_tag="POA77_002519"
     CDS             16951..17169
                     /gene="arfA"
                     /locus_tag="POA77_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDI6365927.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(17166..17576)
                     /gene="mscL"
                     /locus_tag="POA77_002520"
     CDS             complement(17166..17576)
                     /gene="mscL"
                     /locus_tag="POA77_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI6365928.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17706..19082)
                     /gene="trkA"
                     /locus_tag="POA77_002521"
     CDS             complement(17706..19082)
                     /gene="trkA"
                     /locus_tag="POA77_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDI6365929.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19104..20393)
                     /gene="rsmB"
                     /locus_tag="POA77_002522"
     CDS             complement(19104..20393)
                     /gene="rsmB"
                     /locus_tag="POA77_002522"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6365930.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20439..21386)
                     /gene="fmt"
                     /locus_tag="POA77_002523"
     CDS             complement(20439..21386)
                     /gene="fmt"
                     /locus_tag="POA77_002523"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI6365931.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21401..21910)
                     /gene="def"
                     /locus_tag="POA77_002524"
     CDS             complement(21401..21910)
                     /gene="def"
                     /locus_tag="POA77_002524"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6365932.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22040..23164
                     /gene="dprA"
                     /locus_tag="POA77_002525"
     CDS             22040..23164
                     /gene="dprA"
                     /locus_tag="POA77_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6365933.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23136..23609
                     /locus_tag="POA77_002526"
     CDS             23136..23609
                     /locus_tag="POA77_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDI6365934.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23638..24180
                     /locus_tag="POA77_002527"
     CDS             23638..24180
                     /locus_tag="POA77_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDI6365935.1"
                     /translation="MAKSALFTVHNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24185..24757
                     /gene="tsaC"
                     /locus_tag="POA77_002528"
     CDS             24185..24757
                     /gene="tsaC"
                     /locus_tag="POA77_002528"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDI6365936.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24762..25580
                     /gene="aroE"
                     /locus_tag="POA77_002529"
     CDS             24762..25580
                     /gene="aroE"
                     /locus_tag="POA77_002529"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI6365937.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25577..25834
                     /locus_tag="POA77_002530"
     CDS             25577..25834
                     /locus_tag="POA77_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDI6365938.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25810..26364)
                     /locus_tag="POA77_002531"
     CDS             complement(25810..26364)
                     /locus_tag="POA77_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDI6365939.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26837..>27309
                     /locus_tag="POA77_002532"
     rRNA            26837..>27309
                     /locus_tag="POA77_002532"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7022 a         6463 c         7112 g         6712 t
ORIGIN      
        1 aagccgttcc tgctgccgat cgaagacgta ttctccatct ccggtcgtgg taccgttgtt
       61 accggtcgtg tagaacgcgg tatcatcaaa gttggtgaag aagttgaaat cgttggtatc
      121 aaagagactc agaagtctac ctgtactggc gttgaaatgt tccgcaaact gctggacgaa
      181 ggccgtgctg gtgagaacgt aggtgttctg ctgcgtggta tcaaacgtga agaaatcgaa
      241 cgtggtcagg tactggctaa gccgggcacc atcaagccgc acaccaagtt cgaatctgaa
      301 gtgtacattc tgtccaaaga tgaaggcggc cgtcatactc cgttcttcaa aggctaccgt
      361 ccgcagttct acttccgtac tactgacgtg actggtacca tcgaactgcc ggaaggcgta
      421 gagatggtaa tgccgggcga caacatcaaa atggttgtta ccctgatcca cccgatcgcg
      481 atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc gtaccgttgg cgcgggcgtt
      541 gttgctaaag ttctgggcta attacacgtt aattagtttt gaattgaaaa gggcgcttcg
      601 gcgccctttt tgctgcccgt aatttgagct cgaaatattt agtcgtaatt ttgctcgctg
      661 aactatggcg caaataaaac tgtgctattg taatcataac cattctcatt tacactttgc
      721 gcagaatttg aacgggagca atcatgtacg tttgtttgtg taatggtgta agtgacaaaa
      781 aaattcgcca ggcagtccgt cagtttcatc cgcaatcttt ccaacaattg cgtaagttta
      841 ttcctgtggg aaatcaatgc ggtaagtgca ttcgcgccgc gcgcgaagtg atgcaggacg
      901 agttgatgca gatgccagaa tttaaagaga ttgcctgaag accacgctta ttttttgaca
      961 tcccgctagc cccatctacg ctctaatagt ggaagcggag ggactataaa atgaaaggtg
     1021 atgttaaaat cataaattat ctcaataaac tattgggaaa tgagcttgtc gcaatcaatc
     1081 agtattttct ccatgcccgg atgtttaaaa actggggcct gatgcgtctc aatgacgtcg
     1141 aataccatga atccatcgat gagatgaaac acgctgataa atatatcgag cgtattttgt
     1201 ttttagaagg aatccccaac ttacaggatt taggcaaact ggggattggt gaagatgtcg
     1261 aggagatgct gcaatccgac ctgcgattag aactggaagg cgcgaaggat ctccgtgagg
     1321 ctatcgcgta tgccgacagc gttcatgact atgttagccg tgatatgatg atcgagatcc
     1381 ttgccgatga agaaggtcat attgactggc tcgaaactga gctggatctc atcggtaaaa
     1441 tcggtttaca aaattacctg caatcacaaa taaaagttaa agattaacgg cctgccagca
     1501 aactatccca acctacaacg aaacccgcag ccgccagaaa tggcccaaac ggcagcgggt
     1561 tttttaatga ctcttttcct ctcatgagca gaccaacaac tacggctccg caagcgaatg
     1621 aggcagcaag gaaaaccagt cgtggcaaga agggccaggt atgccaggca ccaagggcgg
     1681 cgagaaactt cacatcacca tagcctaatc cttctttatg acgcagtatg cgataccccc
     1741 agtaaatgac agcgaatgtg ccgtaaccaa taatcgcccc ccataacgca tcggctaaac
     1801 cgtcaggatt acaaacctga gagaacaaaa gacctgacca gagtaacggg caggtaaatc
     1861 tgtcaggtaa caagccatgc tttgcatccc ataaaaaaag taggacagat aaacaagcgt
     1921 acaaaatcag aaagggaagg gtagcgtcca taaaacatcc tctgtaattg gagtatcacc
     1981 atcgaataac tccggatgct cagcaacaga gaaatcgctg atttgttaac ctggttccgg
     2041 tgaaaaacgc tatcttcaca accttacaaa tttcgtggaa tacagctcgc aatctgcgcg
     2101 ttgcgattaa tttctacccg atgcttgcgt caggctcaga tttgcgtata atgcgcgggc
     2161 ttgtcgtagt tgacagcagg ttcaatctga acctcagtta gccgaatttg gctacctaac
     2221 aatgctccca atcggggagc tacgtaagaa cggttacact ctcccatcaa tcgtaatggg
     2281 tctgaggagt aatcattttc gtttataaaa taattggagc tctggtctca tgcagaacca
     2341 aagaatccgt atccgcctga aagcgtttga tcatcgtctg atcgatcaag caaccgcgga
     2401 aatcgtcgag actgccaagc gcactggtgc gcaggtccgt ggtccgatcc cgctgccgac
     2461 acgcaaagag cgcttcactg ttctgatctc cccgcacgtc aacaaagacg cgcgcgatca
     2521 gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt gagccaaccg agaaaaccgt
     2581 tgatgctctg atgcgtctgg atctggctgc cggtgtagac gtgcagatca gcctgggtta
     2641 atcaggtcat tgagcgattg agaggttgaa acaatgattg gtttagtcgg taaaaaagtg
     2701 ggtatgaccc gtatcttcac agaagacggc gtttctatcc cagtaaccgt aatcgaagtt
     2761 gaagcaaacc gcgttactca ggttaaagac ctggctaacg atggctaccg tgctattcag
     2821 gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc ctgaagctgg ccacttcgct
     2881 aaagctggcg tagaagctgg ccgtggtctg tgggaattcc gcctggctga aggcgaagag
     2941 ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg acgttaaaaa agttgacgta
     3001 actggcacct ctaaaggtaa aggtttcgca ggtaccgtta agcgctggaa cttccgtacc
     3061 caggacgcta ctcacggtaa ctccttgtct caccgcgttc cgggttctat cggtcagaac
     3121 cagactccgg gcaaagtgtt caaaggcaag aaaatggcag gtcagatggg taacgaacgt
     3181 gtaaccgttc agagccttga cgtagtacgc gttgacgctg agcgcaacct gctgctggtt
     3241 aaaggtgctg tcccgggtgc aaccggtagc gacctgatcg ttaaaccagc tgtgaaggcg
     3301 taaggagata gcaatggaat tagtattgaa agacgcgcag agcgcgctga ctgtttccga
     3361 aactaccttc ggtcgtgatt tcaacgaagc gctggttcac caggttgttg ttgcttatgc
     3421 agctggtgct cgtcagggta ctcgtgctca gaagactcgt gctgaagtaa ctggttccgg
     3481 taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt tctggttcta tcaagagccc
     3541 gatctggcgt tctggtggcg tgacctttgc tgctcgtccg caggaccaca gtcaaaaagt
     3601 taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg tccgaactgg tacgtcagga
     3661 tcgtctgatc gttgtcgaga agttctctgt agaagcgccg aaaactaagc tgctggcaca
     3721 gaaactgaaa gacatggctc tggaagatgt gctgatcatc accggtgagc tggacgaaaa
     3781 cctgttcctg gctgcgcgca acctgcacaa ggttgacgta cgcgatgcaa ctggtatcga
     3841 cccggttagc ctgatcgcct tcgacaaagt cgtaatgact gctgatgctg ttaagcaagt
     3901 tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga aggtgctgcg tgcaccgcac
     3961 gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca ccatcgtact caaagttgct
     4021 aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga aactgtttga agtcgaagtc
     4081 gaagtcgtta acaccctggt agttaaaggg aaagttaaac gtcacggaca gcgtatcggt
     4141 cgtcgtagcg actggaaaaa agcttacgtc accctgaaag aaggccagaa tctggacttc
     4201 gttggcggcg ctgagtaagt cggaggagta atacaatggc agttgttaaa tgtaaaccga
     4261 catctccggg tcgtcgccac gtagttaaag tggttaaccc tgagctgcac aagggcaaac
     4321 cttttgctcc gttgctggaa aaaaacagca aatccggtgg tcgtaacaac aatggccgta
     4381 tcaccactcg tcatatcggt ggtggccaca agcaggctta ccgtattgtt gacttcaaac
     4441 gcaacaaaga cggtatcccg gcagttgttg aacgtcttga gtacgatccg aaccgttccg
     4501 cgaacatcgc gctggttctg tacaaagacg gtgaacgccg ttacatcctg gcccctaaag
     4561 gcctgaaagc tggcgaccag attcagtctg gcgttgatgc tgcaatcaaa ccaggtaaca
     4621 ccctgccgat gcgcaacatc ccggttggtt ctactgttca taacgtagaa atgaaaccag
     4681 gtaaaggcgg tcagctggca cgttccgctg gtacttacgt tcagatcgtt gctcgtgatg
     4741 gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg taaagtagaa gcagactgcc
     4801 gtgcaactct gggcgaagtt ggcaatgctg agcatatgct gcgcgttctg ggtaaagcag
     4861 gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg taccgcgatg aacccggtag
     4921 accacccaca tggtggtggt gaaggtcgta actttggtaa gcacccggta actccgtggg
     4981 gcgttcagac caaaggtaag aagacccgca gcaacaagcg tactgataaa ttcatcgtac
     5041 gtcgccgtag caaataattt tagaggataa gccatgccac gttctctcaa gaaaggtcct
     5101 tttattgacc tgcacttgct gaagaaggta gagaaagcgg tggaaagcgg agacaagaag
     5161 cccctgcgca cttggtcccg tcgttcaacg atctttccta acatgatcgg tttgaccatc
     5221 gctgtccata atggtcgtca gcacgttccg gtatttgtaa ccgacgaaat ggtcggtcac
     5281 aaactgggtg aattcgcacc gactcgtact tatcgcggcc acgctgctga taaaaaagcg
     5341 aagaagaaat aaggtaggag gaagagatgg aaactatcgc taaacatcgc catgctcgtt
     5401 cttctgctca gaaggttcgc cttgttgctg acctgattcg cggtaagaaa gtgtcgcagg
     5461 ctctggatat tttgacctac accaacaaga aagcggctgt actggtcaag aaagttctgg
     5521 aatctgccat tgctaacgct gaacacaacg atggcgctga cattgacgat ctgaaagtta
     5581 cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat tatgccgcgt gcaaaaggtc
     5641 gtgcagatcg catcctgaag cgcaccagcc acatcactgt ggttgtgtcc gatcgctgag
     5701 actctggaga ctagcaatgg gtcagaaagt acatcctaat ggtattcgcc tgggtattgt
     5761 aaaaccatgg aactctacct ggtttgcgaa caccaaagaa ttcgctgaca acctggacag
     5821 cgattttaaa gtacgtcagt acctgactaa ggaactggct aaagcgtccg tatctcgtat
     5881 cgttatcgag cgtccggcta agagcatccg tgtaaccatt cacactgctc gcccgggtat
     5941 cgttatcggt aaaaaaggtg aagacgtaga aaaactgcgt aaggtcgtag cggacatcgc
     6001 tggcgttcct gcacagatca acatcgccga agttcgtaag cctgaactgg acgcaaaact
     6061 ggttgctgac agcatcactt ctcagctgga acgtcgcgtt atgttccgtc gtgctatgaa
     6121 gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt attaaagttg aagttagcgg
     6181 ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac cgcgaaggtc gcgtaccgct
     6241 gcacactctg cgtgctgaca tcgactacaa cacctctgaa gcgcacacca cttacggtgt
     6301 aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg ggtggtatgg ctgctgttga
     6361 acaaccggaa aaaccggctg ctcagcctaa aaagcagcag cgtaaaggcc gtaaataagg
     6421 agcgtcgctg atgttacaac caaagcgtac aaaattccgt aaaatgcaca aaggccgtaa
     6481 ccgcggtctg gcgcagggta cggatgttag cttcggcagc ttcggtctga aagctgttgg
     6541 ccgtggtcgt ctgactgccc gtcagatcga agcagcacgt cgtgctatga cccgtgcagt
     6601 taagcgtcaa ggtaagatct ggatccgtgt gttcccggac aaaccgatca ctgaaaagcc
     6661 gctggcagtg cgtatgggta aaggtaaagg taacgtggag tattgggttg ccttgattca
     6721 gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa gagctggccc gtgaagcatt
     6781 caagctggca gcagcgaaac tgccgattaa aaccaccttt gtaactaaga cggtgatgta
     6841 atgaaagcaa aagagctgcg tgagaagagc gttgaagagc tgaacaccga gctgctgaac
     6901 ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa gtggccagct gcaacagtct
     6961 cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta agactttact gaacgagaag
     7021 gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg tcgcgttgtt agcgacaaaa
     7081 tggagaaatc cattgttgtt gctatcgaac gttttgtgaa acacccgatc tacggtaaat
     7141 tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa caacgaatgc ggtatcggtg
     7201 acgtggttga aatccgcgaa tgccgtccgc tgtccaagac taaatcctgg acgctggttc
     7261 gcgttgtaga gaaagcggtt ctgtaataca gtacactctc tcgatacgaa taaacggctc
     7321 agaaatgagc cgtttatttt ttctacccat atccttgaag cggtgttata atgccgcgcc
     7381 ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc agtagtagtt gacattagcg
     7441 gagcactaaa atgatccaag aacagactat gctgaacgtc gccgacaact ccggtgcacg
     7501 tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt cgctacgcag gcgtaggcga
     7561 catcatcaag atcaccatca aagaagcaat tccgcgtggt aaggtcaaaa aaggtgatgt
     7621 gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt cgcccggacg gttctgtcat
     7681 tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac agcgagcagc ctatcggtac
     7741 gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag aagttcatga aaattatctc
     7801 tctggcacca gaagtactct aaggagcgaa tcatggcagc gaaaatccgt cgtgatgacg
     7861 aagttatcgt gttaaccggt aaagataaag gtaaacgcgg taaagttaag aatgtcctgt
     7921 cttccggcaa ggtcattgtt gaaggtatca acctggttaa gaaacatcag aagccggttc
     7981 cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc cgctattcag gtttccaacg
     8041 tagcaatctt caatgcggca accggcaagg ctgaccgtgt aggctttaga ttcgaagacg
     8101 gcaaaaaagt ccgtttcttc aagtctaaca gcgaaactat caagtaattt ggagtagtac
     8161 gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt aaaaaactca tgactgagtt
     8221 taactacaat tctgtcatgc aagtccctcg ggtcgagaag atcaccctga acatgggtgt
     8281 tggtgaagcg atcgctgaca aaaaactgct ggataacgca gcagcagacc tggcagcaat
     8341 ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct gttgcaggct tcaaaatccg
     8401 tcagggctat ccgatcggct gtaaagtaac tctgcgtggc gaacgcatgt gggagttctt
     8461 tgagcgcctg atcactattg ctgtacctcg tatccgtgac ttccgtggcc tgtccgctaa
     8521 gtctttcgac ggtcgtggta actacagcat gggtgtccgt gagcagatca tcttcccaga
     8581 aatcgactac gataaagtcg accgcgttcg tggtttggat attaccatta ccactactgc
     8641 gaaatctgac gaagaaggcc gcgctctgct ggctgccttt gacttcccgt tccgcaagta
     8701 aggtagggtt actaaatggc taagcaatca atgaaagcac gcgaagtaaa acgcgtagct
     8761 ttagctgata aatacttcgc gaaacgcgct gaactgaaag cgatcatctc tgatgtgaac
     8821 gcttccgacg aagaacgttg gaacgctgtt ctcaagctgc agactctgcc gcgtgattcc
     8881 agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc gtccgcatgg tttcctgcgg
     8941 aagttcgggt tgagccgtat taaggtccgt gaagccgcta tgcgcggtga aatcccgggt
     9001 ctgaaaaagg ctagctggta attgtcacca attgaatcac gggagtaaag acagatgagc
     9061 atgcaagatc cgatcgcgga tatgctgacc cgtatccgta acggtcaggc cgcgaacaaa
     9121 gctgcggtca ccatgccttc ctccaagctg aaagtggcaa tcgccaacgt gctgaaggaa
     9181 gaaggtttta ttgaagattt taaagttgaa ggcgacacca agcctgaact ggaacttact
     9241 ctgaagtatt tccagggcaa agctgttgta gaaagcattc agcgtgtcag ccgcccaggt
     9301 ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta tggcgggtct gggtatcgca
     9361 gttgtttcta cctctaaagg tgttatgact gatcgtgcag cgcgccaggc tggtcttggt
     9421 ggcgaaatta tctgctacgt agcctaatcg gaggaaaaaa tgtctcgtgt tgctaaagca
     9481 ccggtcgttg ttcctgccgg cgttgacgta aaaatcaacg gtcaggttat tacgatcaaa
     9541 ggtaaaaacg gcgagctgac tcgtactctc aacgatgctg ttgaagttaa acatgcagat
     9601 aataccctga ccttcggtcc gcgtgatggt tacgcagacg gttgggcaca ggctggtacc
     9661 gcgcgtgccc tgctgaactc aatggttatc ggtgttaccg aaggcttcac taagaagctg
     9721 cagctggttg gtgtaggtta ccgtgcagcg gttaaaggta atgtgattaa cctgtctctg
     9781 ggtttctctc atcctgttga ccatcagctg cctgcgggta tcactgctga atgtccgact
     9841 cagactgaaa tcgtgctgaa aggcgctgat aagcaggtga tcggccaggt tgcagcggat
     9901 ctgcgcgcct accgtcgtcc tgagccttat aaaggcaagg gtgttcgtta cgccgacgaa
     9961 gtcgtgcgta ccaaagaggc taagaagaag taaggtaaca ctatggataa gaaatctgct
    10021 cgtatccgtc gtgcgacccg cgcacgccgc aagctccagg agctgggcgc aactcgcctg
    10081 gtggtacatc gtaccccgcg tcacatttac gcacaggtaa ttgcaccgaa cggttctgaa
    10141 gttctggtag ctgcttctac tgtagaaaaa gctatcgctg aacaactgaa gtacaccggt
    10201 aacaaagacg cggctgcagc tgtgggtaaa gctgtcgctg aacgcgctct ggaaaaaggc
    10261 atcaaagatg tatcctttga ccgttccggg ttccaatatc atggtcgtgt ccaggcactg
    10321 gcagatgctg cccgtgaagc tggccttcag ttctaaggta gaggtgtaag atggctcaca
    10381 tcgaaaaaca agctggcgaa ctgcaggaaa agctgatcgc ggtaaaccgc gtatctaaaa
    10441 ccgttaaagg tggtcgtatt ttctccttca cagctctgac tgtagttggc gatggtaacg
    10501 gtcgcgttgg ttttggttac ggtaaagcgc gtgaagttcc agcagcgatc cagaaagcga
    10561 tggaaaaagc ccgtcgcaat atgattaacg tcgcgctgaa taacggcact ctgcaacacc
    10621 ctgttaaagg tgttcacacg ggttctcgcg tattcatgca gccggcttcc gaaggtaccg
    10681 gtatcatcgc cggtggtgca atgcgcgccg ttctggaagt cgctggggtt cataacgttc
    10741 tggctaaagc atatggttcc accaacccga tcaacgtggt tcgtgcaact attgatggcc
    10801 tggaaaatat gaattctcca gaaatggtcg ctgccaagcg tggtaaatcc gttgaagaaa
    10861 ttctggggaa ataaaccatg gcaaagacta ttaaaattac tcaaacccgc agtgcaatcg
    10921 gtcgtctgcc gaaacacaag gcaacgctgc ttggcctggg tctgcgtcgt attggtcaca
    10981 ccgtagagcg cgaggatact cctgctattc gcggtatgat caacgcggtt tccttcatgg
    11041 ttaaagttga ggagtaagag atgcgtttaa atactctgtc tccggccgaa ggctccaaaa
    11101 aggcgggtaa acgcctgggt cgtggtatcg gttctggcct cggtaaaacc ggtggtcgtg
    11161 gtcacaaagg tcagaaatct cgttctggcg gtggcgtacg tcgcggtttc gagggtggtc
    11221 agatgcctct gtaccgtcgt ctgccgaaat tcggcttcac ttctcgtaaa gcagcgatta
    11281 cagccgaagt tcgtctgtct gacctggcta aagtagaagg cggtgttgta gacctgaaca
    11341 cgctgaaagc ggctaacatt atcggtatcc agatcgagtt cgcgaaagtg atcctggctg
    11401 gcgaagtaac gactccggta actgttcgtg gcctgcgtgt tactaaaggc gctcgtgctg
    11461 ctatcgaagc tgctggcggt aaaatcgagg aataagtagc agatggctaa acaaccggga
    11521 ttagattttc aaagtgccaa aggtggctta ggcgagctga aacgcagact gctgtttgtt
    11581 atcggtgcgc tgattgtgtt ccgtattggc tcttttattc cgatccctgg tattgatgcc
    11641 gctgtacttg ccaaactgct tgagcaacag cgaggcacca tcattgagat gtttaacatg
    11701 ttctctggtg gtgctctcag ccgtgcttct atctttgctc tggggatcat gccgtatatt
    11761 tcggcgtcga tcattatcca gctgctgacg gtggttcacc caacgttggc agaaattaag
    11821 aaagaagggg agtctggtcg tcgtaagatc agccagtaca cccgttacgg tactctggtg
    11881 ctggcaatat tccagtcgat cggtattgct accggtctgc cgaatatgcc tggtatgcaa
    11941 ggcctggtga ttaacccagg ctttgcattc tacttcaccg ctgttgtaag tctggtcaca
    12001 ggaacgatgt tcctgatgtg gttgggcgaa cagattactg aacgaggtat cggcaacggt
    12061 atttcaatca ttatcttcgc cggtattgtc gcgggactcc cgccagccat tgcccatact
    12121 atcgagcaag cgcgtcaagg cgacctgcac ttcctcgtgt tgctgttggt tgcagtatta
    12181 gtatttgcag tgacgttctt tgttgtattt gttgagcgtg gtcaacgccg cattgtggta
    12241 aactacgcga aacgtcagca aggtcgtcgt gtctatgctg cacagagcac acatttaccg
    12301 ctgaaagtga atatggcggg ggtaatcccg gcaatcttcg cttccagtat tattctgttc
    12361 ccggcgacca tcgcgtcatg gttcgggggc ggtactggtt ggaactggct gacaacaatt
    12421 tcgctgtatt tgcagcctgg gcaaccgctt tatgtgttac tctatgcgtc tgcaatcatc
    12481 ttcttctgtt tcttctacac ggcgttggtt ttcaacccgc gtgaaacagc agataacctg
    12541 aagaagtccg gtgcatttgt accaggaatt cgtccgggag agcaaacggc gaagtatatc
    12601 gataaagtaa tgacccgcct gaccctggtt ggtgcgctgt atattacctt tatctgcctg
    12661 atcccggagt tcatgcgtga tgcaatgaaa gtaccgttct acttcggtgg gacctcactg
    12721 cttatcgttg ttgtcgtgat tatggacttt atggctcaag tgcaaactct gatgatgtcc
    12781 agtcagtatg agtctgcatt gaagaaggcg aacctgaaag gctacggccg ataattggtc
    12841 gcccgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
    12901 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
    12961 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
    13021 ggctagatta gccagccaat cttttgtatg tctgtgcgtt tccatttgag tatcctgaaa
    13081 acgggctttt cagcatggaa cgtacatatt aaatagtagg agtgcatagt ggcccgtata
    13141 gcaggcatta acattcctga tcataagcat gccgtaatcg cattaacttc gatttatggc
    13201 gtcggcaaga cccgttctaa agccatcctg gctgcagcgg gtatcgctga agatgttaag
    13261 atcagtgagc tgtctgaagg acaaatcgac acgctgcgtg acgaagttgc caaatttgtc
    13321 gttgaaggtg atctgcgccg tgaaatcagc atgagcatca agcgcctgat ggatcttggt
    13381 tgctatcgcg gtttgcgtca tcgtcgtggt ctcccggttc gcggtcagcg taccaagacc
    13441 aacgcacgta cccgtaaggg tccgcgcaaa ccgatcaaga aataatcggg gtgattgaat
    13501 aatggcaaag gcaccaattc gtgcacgtaa acgtgtaaga aaacaagtct ctgacggcgt
    13561 ggctcatatc catgcttctt tcaacaacac catcgtgact atcactgatc gtcagggtaa
    13621 cgcgttgggt tgggcaacag ccggtggttc cggtttccgt ggttctcgca aatccactcc
    13681 gtttgcagct caggttgcag cagagcgttg cgctgacgcc gtgaaagaat acggcatcaa
    13741 gaatctggaa gttatggtta aaggtccggg tccaggccgc gaatctacta ttcgtgctct
    13801 gaacgccgca ggtttccgca tcactaatat tactgatgtg actccgatcc ctcataacgg
    13861 ttgtcgtccg ccgaaaaaac gtcgcgtata acgcctcgtt ttccaggttt gttggagaaa
    13921 gaaaatggca agatatttgg gtcctaagct caagctgagc cgtcgtgagg gcaccgactt
    13981 attccttaag tctggcgttc gcgcgatcga taccaagtgt aaaattgaac aagctcctgg
    14041 ccagcacggt gcgcgtaaac cgcgtctgtc tgactatggt gtgcagttgc gtgaaaagca
    14101 aaaagttcgc cgtatctatg gtgtgctgga gcgtcagttc cgtaactact acaaagaagc
    14161 agcacgtctg aaaggcaaca ccggtgaaaa cctgttggct ctgctggaag gtcgtctgga
    14221 caacgttgta taccgtatgg gcttcggtgc cactcgtgca gaagcacgtc agctggttag
    14281 ccataaagca attatggtaa acggtcgtgt tgttaacatc gcttcttatc aggttagtcc
    14341 gaatgacgtt gtaagcattc gtgagaaagc gaagaagcag tctcgcgtga aagccgctct
    14401 ggagctggct gagcagcgtg aaaagccaac ctggctggaa gttgatgctg gcaagatgga
    14461 aggtacgttt aagcgtaagc cggagcgttc tgatctgtct gcggacatta acgaacacct
    14521 gatcgtcgag ctttactcca agtaaagctt agtaccaaag agaggacaca atgcagggtt
    14581 ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt tcgacgcacg
    14641 ccaaggtgac ccttgagcct ttagagcgtg gctttggcca tactctgggt aacgcactgc
    14701 gccgtattct gctctcatcg atgccgggtt gcgcggtgac cgaggttgag attgatggtg
    14761 tactacatga gtacagcacc aaagaaggcg ttcaggaaga tatcctggaa atcctgctca
    14821 acctgaaagg gctggcggtg agagttcagg gcaaagatga agttattctt accttgaata
    14881 aatctggcat tggccctgtg actgcagccg atatcaccca cgacggtgat gtcgaaatcg
    14941 tcaagccgca gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt agcatgcgta
    15001 tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat tcggaagaag
    15061 atgagcgccc aatcggccgt ctgctggtcg acgcatgcta cagccctgtg gagcgtattg
    15121 cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga cctggacaag ctggtcatcg
    15181 aaatggaaac caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg gcaaccattc
    15241 tggctgaaca actggaagct ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag
    15301 aagagaaacc agagttcgat ccgatcctgc tgcgccctgt tgacgatctg gaattgactg
    15361 tccgctctgc taactgcctt aaagcagaag ctatccacta tatcggtgat ctggtacaac
    15421 gtaccgaggt tgagctcctt aaaacgccta accttggtaa aaaatctctt actgagatta
    15481 aagacgtgct ggcttcccgt ggactgtctc tgggcatgcg cctggaaaac tggccaccgg
    15541 caagcatcgc tgacgagtaa ccggatcaca ggttaaggtt ttactgagaa ggataaggtc
    15601 atgcgccatc gtaagagtgg tcgtcaactg aaccgcaaca gcagccatcg ccaggctatg
    15661 ttccgcaata tggcaggttc actggttcgt catgaaatca tcaagacgac tctgcctaaa
    15721 gcgaaagagc tgcgccgcgt agttgagccg ctgattactc ttgccaagac tgatagcgtt
    15781 gctaatcgtc gtctggcatt cgcccgtact cgtgataacg agatcgtggc aaaactgttt
    15841 aacgaactgg gcccgcgttt cgcgagccgt gccggtggtt acactcgtat tctgaagtgt
    15901 ggcttccgtg caggcgacaa cgcgccgatg gcttacatcg agctggttga tcgttcagag
    15961 aaagcagaag ctgctgcaga gtaatctgaa gcaacgtaaa aaaacccgcc ccggcgggtt
    16021 tttttatacc cgtagcatcc ccacttatct acaatagctg tactcttttt gttcatcccc
    16081 tggagtattt atgtggttac ttgaccagtg ggcagagcgt catatagcag aagcgcaagc
    16141 gaaaggtgag tttgataacc tggcaggtag cggcgaacca ttgatactgg atgatgattc
    16201 tcacgtgcca ccggaattac gtgcggggta tcgcttgctg aagaatgccg gttgcttacc
    16261 gccagaactt gagcaacgga gagaagcaat tcagcttctg gatatcctga gaggtatccg
    16321 tcacgatgat ccgcaatatc aagaggttag ccgtcgattg tcattactgg aattgaagct
    16381 gcgacaagct ggattgagta ccgatttttt acgcggcgat tatgctgaca agttgttgga
    16441 caaaatcaac gataactagt ggagtatgta tgtatcgcat tggtgagctg gcaaaaatgg
    16501 cggaagtaac acctgacacg attcgttatt acgaaaaaca gcagatgatg gagcatgaag
    16561 tgcgtactga aggcgggttt cgcctgtata ccgaaagcga tctccagcga ttgaaattta
    16621 tccgccatgc cagacaacta ggtttcagtc tggagtcgat ccgcgagttg ctgtcgatcc
    16681 gcatcgatcc tgaacaccat acctgtcagg agtcaaaagg cattgtgcag gaaagattgc
    16741 aggaagtcga agcacggata gccgagttgc agagtatgca gcgttccttg caacgcctta
    16801 acgatgcctg ttgtgggacc gcccatagca gtgtttattg ttcgattctt gaagctcttg
    16861 aacaaggggc gagtggcgtt aagagtggct gttgattttt tgcactggca ggattacact
    16921 cgcgccgtaa aataaccaat tggagttttt atgagccgat atcagcatac taaagggcag
    16981 ataaaggata atgcgataga agcattacta catgatccct tattccgaca gcgcgtagag
    17041 aaaaataaga aggggaaagg cagttacatg cgaaaaggaa aacatggcaa tcgggggaac
    17101 tgggaggcca gtggcaagaa agtaaatcac ttttttacca ctggccttct gctttcaggc
    17161 gtttgttaag agcggttatt ctgctctttc agcaaatcac gaatttctgt cagtaatact
    17221 tcttctttag ttggtgctgg tgcggctgct ggttcttctt ttttccgatt cagtttgttg
    17281 attagcttaa tcgccataaa gatggcaaag gccacaatca gaaaatcaaa gacgttttga
    17341 atgaagacac cgtaatgcat cacaacagca gggatatccc cctgcgcatc gcgtagcgtg
    17401 acagcaaact gtttaaaatc gatcccgcca attaataagc ccagaggtgg catgatgata
    17461 tcggcaacca gtgaagatac aatcttcccg aatgccgcac cgataatgac acccaccgcc
    17521 aaatccacca cgttcccgcg catcgcaaat tcgcgaaatt ctttaataat gctcatgtta
    17581 ttctccctat gaagccgaca accataagtc taacaaatgt taagcctttt tcctgccagg
    17641 aatagattta attaatcctt gataatcaaa gggaaataat aaggcgtcat tagacgcctt
    17701 attaattaca agaagaaagg acttggctgg aagagtcttt cgacgtcggt aataaacttt
    17761 ttatctgtga ggaacataat tacgtgatcg ccttgctcaa tgcgcagatt gtcattggca
    17821 atcatcacgt cgtttccacg taccaccgct ccaataatcg ttcctggcgg tagcttgatt
    17881 tcgtcaatga ctctgccgac aacgcgtgat gtgctttcat caccgtgagc aacggcttca
    17941 atagcttctg ctacgccgcg gcgcaatgag gaaacaccaa caatatctgc ttttcgcaca
    18001 tggctaagca acgcagaaat agttgcttgt tgtggtgaaa ttgcaatatc gatcacgctc
    18061 ccctgaacca gatccacata agcgcgacgc tggatcaata ccatcacctt tttcgcaccc
    18121 atacgtttgg caagcatggc ggacatgata ttggcctcgt catcgttggt gacggcaata
    18181 aacagatcaa cttgatcgat atgttcttcg gccagcagtt cttgatccga cgcatcacca
    18241 aaaaagacga tcgtattctg caacttttcc gccagttcgg cagcgcgctg ctgattacgt
    18301 tcgatgagtt taacgctgta atctttttcc agacgacgcg ccagccctgc accgatatta
    18361 ccgccaccaa ccagcatgat ccgcttatac ggtttttcca gtcgctgtaa ttcactcatc
    18421 accgcgcgga tatgctgtga agcggcaata aagaacactt catcacccgc ttcaacaatg
    18481 gtcgaacctt gcggacgaat agggcgatcg tggcggaaaa ttgctgccac acgagtatcg
    18541 atatgtggca tatgttcgcg catggtcgaa agtgcgttac caatcagcgg gccgccataa
    18601 taggctttaa ccacagcgag gctgacttta ccctcagcga agttcaccac ctgcaatgcg
    18661 ccgggatact caatcagtcg gtaaatatta tcgataacca actgctctgg tgcgatcaga
    18721 tgatcaatcg gtacagcatc tgaatgaaat agcttatcgg catcgcgcac gtagtctggt
    18781 gagcggatgc gagcgatgcg attaggggtg ttgaaaagtg agtaggctac ctggcaggca
    18841 accatattgg tttcatctga actggttaca gcaaccagca tatcggcgtc gtcggcacct
    18901 gcctcccgca atacgcgtgg atgagagcca tgcccctgca cgactcgcag gtcaaattta
    18961 tcctgcaagg tccgcagacg ctcaccgttg gtatcgacaa cagtgatatc gttgttctcg
    19021 ccaaccaggt tttccgccag tgtgccgcca acctggccgg cacccagaat gataattttc
    19081 atcagtcgcg acccgttatc tcatcacttt ttgattagct tagcgtaaaa gaagccgtcg
    19141 ccctcttcgg caccaggcag attttgttta cccggttgct ctggtgttcc tgtttcgcaa
    19201 agttcggcat cagcggtacg ttgcaaaaag gctttaatct gcaggctatt ctcttccggt
    19261 aacaccgaac aggtggcata gaccagggtt ccaccggatt ttaaatgcgg ccaaatggcg
    19321 tcgagaattt cagactgcaa ttgcgcgagt tccgggatat cgcgatcgcg acgtaaccat
    19381 ttaatatccg gatggcgacg aatcacaccg gttgctgaac aaggcgcatc taataaaatg
    19441 cgatcaaact gttgctcgcc acaccattgg gaagggtagc ggccatcacc ttgtttcacg
    19501 gtcgctttca taccaaggcg ttttaaattg tcgtaaacgc gagagaggcg ctgttcgtca
    19561 atatcaaccg caacaacctg cgcttctggt gccacctcaa ggatatgcgt tgttttaccg
    19621 ccgggggcgg cacaaagatc cagaatgtgt tcaccgtttt gtggcgcaag ccaggtcatg
    19681 caaccttgtg ctgatgcatc ctgaacggta acccatccgt cttcaaaacc aggtagcgca
    19741 tgaacaggtg caggtgtttc cagccgtaca gcatcagggt aatccgcatg cgggaaacct
    19801 ttcattcctg cttcatccaa caatgcaagc cagctgtcgc gggaatgatg cgtacgatta
    19861 acgcgcagcc acattggcgg acgctggtta ttggcttcga cgatggattg ccactgctct
    19921 ggatacgctt tttgcagacg cttcagcaac caggaaggat gcagataacg tgcatcactg
    19981 gcattaaact cggctaataa ctcttcttgc tgacgctgga actggcgtaa tacaccgttt
    20041 atcaaccctt taagttgcgg acgcttaatt gcgatagcgc cttcaaccgt ttcagccagc
    20101 gcagcatgag gtggaatgcg ggtataaagc agttgataca aaccaaccat aatcaggtaa
    20161 tgcacggtcc gctgtttgcc ggtcatcgga cgggccatta acttattaat cagccagtct
    20221 aactgcgaaa gcgtacgcag tacgccaaag cacaactctt gaagaagtgc tttgtcttta
    20281 tcggaaactt tttgctggag cggtggcaga atgttgctta atgattgccc ttgctcgacg
    20341 acttgttcaa cggcctgggc cgccatgcta cgtaaattac gttgtttttt cataagtata
    20401 aaaacgcccg gcaagaccgg gcttagaaga gtggacaatc agaccagacg gttgcccgga
    20461 acaaaccatt cccgacgaga gttcaggagg tcttgcgcgc tcatcgcttt cttacccgca
    20521 ggttgtaacg agagcaggtt caggatgcca tcaccagtcg caacctgaat gccttgtttg
    20581 ttggcttcaa ggatcgttcc tggtgcagcg ttggttgccg tatcaatgac cgatgctttc
    20641 cagactttaa ccggctgtcc ttcaatttcc agccagctca ttggccatgg attgaaagcg
    20701 cgaatgcagc gttcaagctg tgctgccgaa agtgaccagt caatacgcgc ttcttcttta
    20761 ctcaactttt cggcgtaagt gacaagagtt tcgtcctgaa cttctggttt cgccgtgccg
    20821 tctgccagtt gttttaacgt ggtgataagc ccttgtgggc caagctctgc cagcttgtcg
    20881 tacagcgtac cactggtatc ttctgcagta atcgggcagg agagcttata gagcatgtca
    20941 ccggtgtcta aaccaacatc catttgcata atggtcacac cagtttctgc atcacccgcc
    21001 catagtgagc gttggattgg tgcagcgccg cgccagcgtg gcagcagtga accatgaacg
    21061 ttgatacagc caagacgcgg catctccagc acagctttag gcagaattaa accataggcg
    21121 acgacgacca taacatcagc ctgcagatcg gcgaccagtt gctggttttc ttgtggacgc
    21181 agggaaacag gttgaaaaac gggcagacct ttttcctcag ccagaacttt aatcgggctg
    21241 ggcatcagtt ttttaccgcg tcctgccggt cggtctggct gggtgaacac gccaacgacg
    21301 ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg caaagtcagg tgtacccgca
    21361 aaaataatac gtagtgattc tgacacgtta gttcttatcc ttaagcccgg gctttcagac
    21421 gatccagttt ttcaactttc tgacgaatac gttgttgttt caacggtgac agataatcca
    21481 taaacagttt gccgaccagg tgatccatct catgctgaat acagatggct aacagaccgt
    21541 ctgcttccag ttcaaatggt ttaccgtcgc ggtccagggc gcgaatttta actttctctg
    21601 cgcgtggcac taaagcacgt tgttcaggga tcgacaggca accttcttca atgcctgttt
    21661 cgccgctttt ttctaaaagc tccggattga ttaacaccag ccgttcgtca cggttttccg
    21721 aaacatcaat aacaatgata cgttgatgga tatcaacctg ggttgccgcc aggccaatac
    21781 cttcttctgc gtacatcgtc tcgaacatat catcgacgat acgctgaatt tctgcattca
    21841 cttcttctac cggtttagca actttgcgaa gccgctcgtc cggaatatgt aacacttgca
    21901 aaactgacat aaatctccag agatgtgttc aggagttaga aagattattt cttctattct
    21961 agacaaatcc ccctctgatt gacagcatca ctgaccaatc gcaaagattg ctaaggctgc
    22021 ttatggcagg gagataagga tggtcgatac agaaatttgg ctgcgtttaa tgagtatcag
    22081 cagcttgtac ggcgatgata tggtccgtat agctcactgg ctggcaaaac agtcgcaaat
    22141 tgatgcggtt ggattgcagc aaacagggct tacattgcgg caggcacaac gctttctttc
    22201 atttccgcga aagagtatcg aaagctcact ttgttggttg gagcaaccca accatcattt
    22261 aatccctgcg gacagcgaat tttatcctcc tcaacttctg gtgacgacag attacccagg
    22321 cgcactgttt gttgaaggag aactgcacgc gctgcattca tttcagcttg ccgtagtggg
    22381 gagtcgggcg cattcatggt atggcgagcg atggggacgg ttattttgcg aaactctggc
    22441 gacgcgtgga gtgacaatta cgagtggact ggcgcgtgga atcgatggtg tggcgcataa
    22501 agctgcctta caggtaaatg gcgtcagcat tgctgtattg gggaatggac ttaataccat
    22561 tcatccccgc cgccatgccc gactggctgc cagtttgctt gaacatggcg gagctctcgt
    22621 ctcggaattt cccctcgatg ttccacccct tgcttacaat ttcccacgaa gaaatcgcat
    22681 tatcagtggt ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca gtggttcgct
    22741 agtgacagca cgttgtgcgc ttgagcaggg gcgtgaagtt tttgccttgc caggtccaat
    22801 agggaatccg ggaagcgaag ggcctcactg gttaataaaa caaggtgcga ttcttgtgac
    22861 ggaaccggaa gaaattctgg aaaacttgca atttggattg cactggttgc cagacgcccc
    22921 tgaaaattca ttttattcac cagatcagca agacgtggca ttgccatttc ctgagctcct
    22981 ggctaacgta ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc
    23041 tgtgccagag gtagttactc aactactcga actggagtta gcaggatgga tcgcagctgt
    23101 acccggcggc tatgtccgat tgaggagggc atgccatgtt cgacgtacta atgtatttgt
    23161 ttgaaaccta tattcacaca gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg
    23221 atcttaccga cgcaggattt gatcgagaag atatctacaa tgccctgctt tggctggaaa
    23281 agcttgcgga ttatcaggaa ggactggcag aaccgatgca actggcctct gatccactct
    23341 ccatgcgtat ttatacccct gaggagtgtg aaagactgga tgccagctgt cgtggttttc
    23401 tgcttttcct tgagcagatt caggtgctca accttgaaac tcgtgaaatg gtgatagagc
    23461 gagtgctcgc gctggataac gcagagttcg aactggatga tctgaaatgg gtaatcctga
    23521 tggtgttgtt caatattccg ggctgcgaaa atgcgtacca gcaaatggaa gaattactct
    23581 ttgaagtgaa tgaaggtatg ctgcattaat catttcttta attcagcata agttgttatg
    23641 gcgaaatcag cactgttcac ggtgcataat aatgagtcct gcccaaagtg cggggctgaa
    23701 ctggttattc gatccgggaa acacggtccg tttcttggat gctcacagta tccggcgtgt
    23761 gactacgtcc gtcctctgaa atcttcagcg gatggacata ttgtcaaagt tctggagggg
    23821 caggtttgcc ctgcatgtgg cgcaaatctg gtattacgcc agggacgctt tggtatgttt
    23881 attggttgca gtaactaccc tgaatgcgaa cataccgaac ttatcgataa accggacgaa
    23941 acagcaatta catgccccca atgccggacg ggccatctgg tccagcgccg ctcccgttat
    24001 ggcaaaacat ttcactcctg tgatcgctac ccggagtgtc aatttgccat taacttcaaa
    24061 cccatagctg gagaatgccc tgagtgtcat tatccactac tcatcgaaaa gaaaaccgcg
    24121 cagggtgtaa aacacttttg tgccagtaaa caatgtggaa agccggtttc ggcggaataa
    24181 taacgtgaat aataacctgc aaggagacgc tatcgcagct gcgatagatg tcctcaatga
    24241 agaacgtgtc atcgcctatc caacggaagc cgttttcggt gtcgggtgcg atcctgatag
    24301 cgaaacagca gtgatgcgac tgttggagtt aaaacagcgt ccggttgata aggggctgat
    24361 tttaatcgcg gcaaattacg agcagcttaa accctatatt gatgacacca tgctgactga
    24421 tgcgcagcgt gaaaccattt tttcccgctg gccaggtcct gtcacctttg tctttcccgc
    24481 gcctgcgaca acaccgcgct ggttgacggg ccgctttgat tcgcttgctg tacgagtcac
    24541 cgaccatccg ttggtggttg ctttgtgcca ggcttatggt aaaccgctgg tttctaccag
    24601 tgccaacttg agtggattgc caccttgtcg aacagtagac gaagttcgcg cacaatttgg
    24661 cgcggcgttc ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg
    24721 cgatgccctg acgggtgaac tgtttcgaca ggggtaacat aatggaaacc tatgctgttt
    24781 ttggtaatcc gatagcccac agcaaatcgc cattcattca tcagcaattt gctcagcaac
    24841 tgaatattga acatccctat gggcgcgtgt tggcacccat caatgatttc atcaacacac
    24901 tgaacgcttt ctttcgtgct ggtggtaaag gtgcgaatgt gacggtgcct tttaaagaag
    24961 aggcttttgc cagagcggat gagcttactg aacgggcagc gttggctggt gctgttaata
    25021 ccctcaagcg gttagaagat ggacgcctgc tgggtgacaa taccgatggt ataggtttgt
    25081 taagcgatct ggaacgtctg tcttttatcc gtcctggttt acgtattctg ctcatcggcg
    25141 ctggtggagc atctcgcggc gtactactgc cacttctttc cctggactgt gcggtgacaa
    25201 tcactaatcg gacggtatcc cgcgcggaag agttggctaa attgtttgcg cacactggca
    25261 gtattcaggc gttgggtatg gacgaactgg aaggtcatga gtttgatctc attattaatg
    25321 caacatccag tggcatcagt ggtgatattc cggcgatccc gtcatcgctc attcatccag
    25381 gcatttattg ctatgacatg ttctatcaga aaggaaaaac tccttttctg gcatggtgtg
    25441 agcagcgagg ctcaaagcgt aatgctgatg gtttaggaat gctggtggca caggcggctc
    25501 atgcctttct tctctggcac ggtgttctgc ctgacgtaga accagttata aagcaattgc
    25561 aggaggaatt gtccgcgtga atcaggccat ccagtttccg gacagagaag agtgggacga
    25621 gaataaaaaa tgtgtatgtt ttcccgctct cgtgaatggc atgcaactga catgtgcgat
    25681 ctctggcgag agtctggcgt atcgttttac tggagatacg ccagaacagt ggttagcgag
    25741 ttttcgtcag catcgctggg atctggaaga agaagcggaa aacttaattc aggaacaaag
    25801 tgaagatgat caaggctggg tctggttacc ctgatccaga tattcgtcct tccatttcac
    25861 gtaattattc gcggaatagc gtaacccagc cttctcttca tcacttaacg ggcggatctg
    25921 tttgacgggg ctaccgagat acagatatcc gctctccagc cgtttatttt gtgggaccag
    25981 actacccgca ccaatcatca catcatcttc tactattgcg ccatcaagta aaattgagcc
    26041 catcccaacc aaaactcgat tgccaatggt gcagccgtgg agcatcacct tgtgaccaac
    26101 agtgacatct tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg atttatgagt
    26161 gacatgcaac atactgccat cctggatatt ggtgcgtgct ccgatctgta cataatgtac
    26221 atctccacga atcacaacga gcggccagat ccccacatca tcagccagac gaacgtcacc
    26281 aatcacgaca ctgctatcgt cgatcattac gcgctgaccg atttgtggaa aaagatcgcg
    26341 gtatgggcgt aaaacatcag acatacttac ctcagcaata aatgatttac taatgacttt
    26401 gggggcatta ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg ttttgtgtgc
    26461 gaatgcagca aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa aagtacttgt
    26521 gcaaaaaatt gggatcccta taatgcgcct ccgttgagac gacaacgtga aacacttcac
    26581 aagatggtcg taacaacgaa gagaaaaaat cctgaaattc agggttgact ctgaaagagg
    26641 aaagcgtaat atacgccacc tcgcgacagt gagctgaaag ccgcgtcgca actgctcttt
    26701 aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga
    26761 cgaaaaatga ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt
    26821 tgagcatcaa acttttaaat tgaagagttt gatcatggct cagattgaac gctggcggca
    26881 ggcctaacac atgcaagtcg aacggtaaca ggaagaagct tgcttctttg ctgacgagtg
    26941 gcggacgggt gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg
    27001 gtagctaata ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat
    27061 cggatgtgcc cagatgggat tagcttgttg gtggggtaac ggctcaccaa ggcgacgatc
    27121 cctagctggt ctgagaggat gaccagccac actggaactg agacacggtc cagactccta
    27181 cgggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg
    27241 tgtatgaaga aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt
    27301 aataccttt
//
LOCUS       DAMZII010000062        27029 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZII010000062 DAMZII010000000
VERSION     DAMZII010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.062"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(33..1487)
                     /gene="xylB"
                     /locus_tag="POA77_002533"
     CDS             complement(33..1487)
                     /gene="xylB"
                     /locus_tag="POA77_002533"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDI6365940.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGVRSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQPHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="POA77_002534"
     CDS             complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="POA77_002534"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDI6365941.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3247..4239
                     /gene="xylF"
                     /locus_tag="POA77_002535"
     CDS             3247..4239
                     /gene="xylF"
                     /locus_tag="POA77_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6365942.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4317..5858
                     /gene="xylG"
                     /locus_tag="POA77_002536"
     CDS             4317..5858
                     /gene="xylG"
                     /locus_tag="POA77_002536"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDI6365943.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5836..7017
                     /gene="xylH"
                     /locus_tag="POA77_002537"
     CDS             5836..7017
                     /gene="xylH"
                     /locus_tag="POA77_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDI6365944.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIILTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7095..8273
                     /gene="xylR"
                     /locus_tag="POA77_002538"
     CDS             7095..8273
                     /gene="xylR"
                     /locus_tag="POA77_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDI6365945.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8381..9205)
                     /locus_tag="POA77_002539"
     CDS             complement(8381..9205)
                     /locus_tag="POA77_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDI6365946.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9525..11555
                     /gene="malS"
                     /locus_tag="POA77_002540"
     CDS             9525..11555
                     /gene="malS"
                     /locus_tag="POA77_002540"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI6365947.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANVVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11733..12986
                     /gene="avtA"
                     /locus_tag="POA77_002541"
     CDS             11733..12986
                     /gene="avtA"
                     /locus_tag="POA77_002541"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDI6365948.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13138..13611)
                     /locus_tag="POA77_002542"
     CDS             complement(13138..13611)
                     /locus_tag="POA77_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI6365949.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13713..14561)
                     /gene="yiaJ"
                     /locus_tag="POA77_002543"
     CDS             complement(13713..14561)
                     /gene="yiaJ"
                     /locus_tag="POA77_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDI6365950.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14762..15760
                     /gene="yiaK"
                     /locus_tag="POA77_002544"
     CDS             14762..15760
                     /gene="yiaK"
                     /locus_tag="POA77_002544"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDI6365951.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15772..16239
                     /locus_tag="POA77_002545"
     CDS             15772..16239
                     /locus_tag="POA77_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI6365952.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16357..16830
                     /gene="yiaM"
                     /locus_tag="POA77_002546"
     CDS             16357..16830
                     /gene="yiaM"
                     /locus_tag="POA77_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDI6365953.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16833..18110
                     /gene="yiaN"
                     /locus_tag="POA77_002547"
     CDS             16833..18110
                     /gene="yiaN"
                     /locus_tag="POA77_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDI6365954.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18123..19109
                     /gene="yiaO"
                     /locus_tag="POA77_002548"
     CDS             18123..19109
                     /gene="yiaO"
                     /locus_tag="POA77_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDI6365955.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYDTSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTH
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19113..20609
                     /gene="lyxK"
                     /locus_tag="POA77_002549"
     CDS             19113..20609
                     /gene="lyxK"
                     /locus_tag="POA77_002549"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDI6365956.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20606..21268
                     /gene="ulaD"
                     /locus_tag="POA77_002550"
     CDS             20606..21268
                     /gene="ulaD"
                     /locus_tag="POA77_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI6365957.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            21261..22121
                     /locus_tag="POA77_002551"
     CDS             21261..22121
                     /locus_tag="POA77_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDI6365958.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22115..22810
                     /gene="araD"
                     /locus_tag="POA77_002552"
     CDS             22115..22810
                     /gene="araD"
                     /locus_tag="POA77_002552"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI6365959.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22842..22943)
                     /locus_tag="POA77_002553"
                     /pseudo
     CDS             complement(22842..22943)
                     /locus_tag="POA77_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(23157..23897)
                     /locus_tag="POA77_002554"
     CDS             complement(23157..23897)
                     /locus_tag="POA77_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDI6365960.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            24021..24995
                     /locus_tag="POA77_002555"
     CDS             24021..24995
                     /locus_tag="POA77_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6365961.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(24992..25993)
                     /locus_tag="POA77_002556"
                     /pseudo
     CDS             complement(24992..25993)
                     /locus_tag="POA77_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(26050..27018)
                     /locus_tag="POA77_002557"
     CDS             complement(26050..27018)
                     /locus_tag="POA77_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HDI6365962.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
BASE COUNT         6638 a         6966 c         6815 g         6610 t
ORIGIN      
        1 tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga
       61 tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt
      121 agatgcggct gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc
      181 gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg
      241 taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcacc
      301 ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg
      361 ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca
      421 tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc
      481 tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct
      541 gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcgaccca atccagacac
      601 gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag
      661 ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc
      721 gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca
      781 gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
      841 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
      901 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
      961 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     1021 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     1081 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     1141 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     1201 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     1261 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     1321 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     1381 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     1441 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     1501 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     1561 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat
     1621 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     1681 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     1741 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     1801 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     1861 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     1921 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     1981 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     2041 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     2101 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     2161 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaag ccgattttat
     2221 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     2281 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     2341 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     2401 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     2461 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     2521 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     2581 ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     2641 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     2701 caaacatgtc cgccccgttc cagcagaagg tgtgccagta gcaggcggca aaacgcaagt
     2761 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     2821 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     2881 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     2941 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     3001 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     3061 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     3121 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     3181 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     3241 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     3301 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa
     3361 cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg cgcgaaagta
     3421 tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgatt
     3481 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt
     3541 gtaaaagaag ccaaacaaga aggcattaaa gtactagctt acgaccgtat gattaacgat
     3601 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     3661 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     3721 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     3781 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
     3841 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
     3901 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
     3961 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
     4021 caaactatga cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc
     4081 gttgagttgg gcaatggtca ggaaccaaaa gcagatacca cactgaataa tggcctgaaa
     4141 gatgtgccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagacacg
     4201 gtagttaaag acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag
     4261 cgggggcgtg atttctctcc atgccgcgtg aattaattgg cttaggtgga gtcgttatgc
     4321 cttatctact tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata
     4381 acgtcagctt gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg
     4441 gtaaatcaac gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg
     4501 aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag
     4561 gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgta ctggaaaata
     4621 tcttcctggg taacgaaata acccacaatg gtattatgga ttatgacctg atgacgctac
     4681 gctgtcagaa gctgctcgca caggtcagtt tatccatttc accagatacc cgcgtgggag
     4741 atttagggct tgggcaacaa caactggttg aaatagccaa ggcacttaat aaacaggtgc
     4801 gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac
     4861 tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac
     4921 tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacggt cagcacattg
     4981 gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag
     5041 agttaaccgc gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg
     5101 aacatctgac ggcatggcat ccggttaatc gtcatattaa aagagttaat gatgtctcgt
     5161 tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg
     5221 agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg
     5281 atggcaaaca ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg
     5341 tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta
     5401 ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc
     5461 aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgaccttg
     5521 ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgctcgc tgtctgttac
     5581 ttaacccgcg catcctcatt cttgatgaac ctaccagggg tatcgatatt ggcgcgaaat
     5641 acgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct
     5701 cttccgaatt acctgaagtg ctcggcctta gcgatcgggt actggtgatg catgaaggga
     5761 aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat
     5821 tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca
     5881 tccggtggtt tttctgggct gaagtcactg aatttgcagg tcttcgtgat gattgcagct
     5941 atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt
     6001 aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg
     6061 ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt
     6121 ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattctg
     6181 acgttggttc tgggactgct tctcggtgcg tggaacggat ggtgggtcgc gtaccgtaaa
     6241 gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc
     6301 atcaccaacg gcacgactgt atcccccacc agcgccgcga tgtcacaaat tggacaaagc
     6361 tatctccccg ccagtaccgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt
     6421 tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tacagtctcc ggcctctacc
     6481 gcagtagtcg gtcgccaggc tttaaccgct atcatcgtat taggcgcaat ctggctgttg
     6541 aatgattacc gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt actcggcgga
     6601 atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctt
     6661 gaagcggcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt
     6721 aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca
     6781 ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc
     6841 agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct
     6901 tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa
     6961 ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt
     7021 tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca
     7081 gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata
     7141 aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggcg tcacaatcgg
     7201 aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt
     7261 taggagatgg cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg
     7321 tcgacgtccc cattgttggg gttggcggct cgtatcacct tgccgaaagt tacccacccg
     7381 ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag
     7441 agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggt aaacgttggg
     7501 ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg
     7561 tttatcaggg gttagaaacc gcgccagaga actggcaaca tgcgcaaaat cggctggcag
     7621 actggctaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgggcgc
     7681 ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga
     7741 ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg
     7801 ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata
     7861 aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct
     7921 caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta
     7981 atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct
     8041 ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgcc atgattcatg
     8101 ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga
     8161 tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat
     8221 atgacacaac gccaaaagag tatcgcgatg taaatagcga ggtcatgttg tagcgatgat
     8281 gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct
     8341 tatccgaccg acaaatcaga taaggctcaa ggaaatgcaa ttacatatgc gccgcgatta
     8401 accgttggtt atcctggtac attgcgaaca ggtagttatt ataactcttc cctttggtcg
     8461 agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca
     8521 gctgcgcacg cgatttacgg aacgaagagt aagccgggtg cgtattcagg ttagtgacat
     8581 aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg
     8641 cattggtaca acgtcctttc atgcatttca tgccgaacag attgttattg ttgcgcgcca
     8701 gcttcgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtaggga
     8761 taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact
     8821 tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gaccattggc
     8881 cctgatactg ttttgaaatc agccagttac gttctgcagt aatggctgca ttttggctgg
     8941 taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt
     9001 ttcgcaaatc aggaagtgaa ctactctttg cactattgcg agaatactct tgtttactgc
     9061 ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg
     9121 tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat
     9181 atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc
     9241 ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga
     9301 gcaccccaag gaatagtcca aatctgaaac tatgtcacgt gttaacgatt cagattggcg
     9361 ctaaatcgca gaaaatgtgg ggtttatcgc aaaattcagc cgttttttgc gcgagatcgc
     9421 tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt ctggcttatc
     9481 gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct
     9541 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
     9601 ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta
     9661 cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac
     9721 tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca
     9781 gagacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt
     9841 ccatccagaa cgccgccgaa ccggtagcaa acgtggtccg tgaatgcccg aaatgggatg
     9901 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
     9961 attacagcca gcaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta
    10021 ccagcaacgg tttactcctg ctggaacggg cagaaactga cgcccctgcc cctttcgact
    10081 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
    10141 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
    10201 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
    10261 tatggataag cgccccattt gagcaaattc acggctgggt cggcggcggt acaaaaggcg
    10321 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
    10381 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
    10441 gtattctctt tgatgtcgtg atgaaccaca ccggctatgc cacgctggcg gatatgcagg
    10501 agtatcagtt tggcgcgtta tatctttctg gtgacgaggt gaaaaaaacg ctgggtgaac
    10561 gctggagcga ctggaaacct gccgccgggc aaacctggca tagctttaac gattacatta
    10621 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata
    10681 tcggcgatta cgacaatcct ggattcgacg atctcactat gtcgctggcc tttttgccgg
    10741 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
    10801 atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa
    10861 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
    10921 agttgcccgc ctggcagcaa ctgaaaaccg aagccagcgc cgcgcttcgc gaatggaaaa
    10981 aagctaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg
    11041 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
    11101 attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc
    11161 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
    11221 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
    11281 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
    11341 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
    11401 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
    11461 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
    11521 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
    11581 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg caggataaag
    11641 caccgctcac tctccaacag accgatttgc accccagcaa atgtagcgtt attgttacct
    11701 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
    11761 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc
    11821 ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact
    11881 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt
    11941 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
    12001 agttgggttg ggatatcgaa gcacagaata ttgcactaac aaacggcagc cagagcgcgt
    12061 ttttctactt atttaatctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
    12121 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
    12181 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
    12241 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
    12301 atccaacagg caatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
    12361 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
    12421 tcagtgaagc gcgcccgcta tggaatccga atatagtgct gtgcatgagt ctttccaagc
    12481 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
    12541 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
    12601 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
    12661 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaagatcgct
    12721 gcctaattca taaaccagaa ggagccattt tcctctggct ttggtttaag gatttgccca
    12781 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
    12841 acaacttctt cccagggctg gacaaaccgt ggccgcatac gcatcaatgt atgcgcatga
    12901 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
    12961 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta ttaggcctac
    13021 atgatttctg caatatattg aatttgcaag aatttgtggg ccggataagg cgtttacgcc
    13081 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aaacgtttca
    13141 aacaggctgc tgccgtagcc gatgccgctg cactttctcg acatccatgc actgcaacgc
    13201 ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc acagatcgca
    13261 cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc
    13321 cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat gcccatgttc
    13381 gcggcttatc gcgttaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca
    13441 ggctactgcc gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc
    13501 tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca
    13561 ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcactcc
    13621 tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg
    13681 aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgccctg gtcgtccctg
    13741 acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga
    13801 tttttctccc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc
    13861 acccgcccat gaatattaaa caccggaaca gcaatacagg agacgccgag ttcgttttct
    13921 tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc
    13981 agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt ttcccagtat
    14041 gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc ggaacagtag
    14101 agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt gggttccagc
    14161 ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt ttcaccagtg
    14221 gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag cgaagacagc
    14281 gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc ggcgggcgcg
    14341 gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt actcttattt
    14401 aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc
    14461 tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc tttttcctgc
    14521 gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg tcctgatggt
    14581 agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac ttgattttta
    14641 tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata tatttgaaat
    14701 caagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa ggaagcctgg
    14761 gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa tttcacgcgg
    14821 cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca ccgaatccgg
    14881 cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg aaaacggcga
    14941 tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg aacagtggga
    15001 tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg ccattgaact
    15061 ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact ggatgcgcgg
    15121 cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct ggaccaactc
    15181 catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc attggcacta acccgctgat
    15241 cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga tgttctctta
    15301 cggcatgtta gaagttaacc gcctggcggg ccgtcagctc ccggtcgatg gtggctttga
    15361 tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc gcattttgcc
    15421 gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg ctactctcct
    15481 ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat acggcatttc
    15541 acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc gcgatgccaa
    15601 gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa accaggccat
    15661 ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta acggtattac
    15721 cgttgatgac agcgtgtggg ccaaaatcca ggcgttataa ggagataagt catgattttt
    15781 ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa ggcgctcgat
    15841 tttctgcgcg ctaccgattt caacgccctg gagccgggcg ttgtcgaaat cgacggcaaa
    15901 aatatttatg cgcaaattat cgatttaacc acacgcgaag cggtggaaaa ccgtccggaa
    15961 gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg gtgaagagaa aatcggcatt
    16021 gctattgata cgggaaataa taaagtcagc gaatcactat tagagcagcg cgatattatt
    16081 ttttatcatg acagtgaaca tgaatcattt atagaaatga taccgggcag ctacgcaatt
    16141 ttctttccgc aggatgttca ccgaccaggt tgtattttgc aaacagcctc tgagatacga
    16201 aaaattgtgg ttaaagtcgc attaacggcg ctgaattaat cgaataatcg tctacgccag
    16261 aacgactggg taatgttatt gctctaacta tattaataat ttaggttgct gcacatgcaa
    16321 cttgaattat gacagacata gcctcaagga atagctatga aaaaaatact tgaagcaata
    16381 ctggcgatta atctcgccgt actttcctgt attgtgttta taaacattat tttaagatat
    16441 ggttttcaga caagcatttt atctgtagat gaattgtcac gttatttatt tgtctggtta
    16501 acatttattg gcgcgattgt ggcttttatg gataacgccc acgttcaggt cacctttctg
    16561 gtggagaaac tctcccccgc atggcaacga cgagtcgctc tggttaccca ttccttaatc
    16621 ttatttattt gtggcgcact ggcctggggc gcaacgctaa aaacaattca ggactggagc
    16681 gattattcac cgatcctcgg tttacccatc ggcctgatgt atgccgcttg tctgcccacc
    16741 agcctcgtta tcgcattctt tgaactgcgt catttatatc aactcatcac gcgtagcaat
    16801 tctctaacgt cgccaccgca aggagcttaa tcatggctgt gctgattttt ctgggctgtc
    16861 tgttgggtgg tattgctatc ggtttgccta ttgcctgggc actgttgttg tgcggggcgg
    16921 cgttaatgtt ctggctggac atgtttgatg tccagatcat ggcacaaacg ctggtgaacg
    16981 gagccgatag cttctccctg ctggcgattc cgttctttgt gctggcgggt gaaatcatga
    17041 atgcgggcgg cttgtcaaaa cgcattgttg acctgccgat gaaactggtg gggcataaac
    17101 caggcgggct gggctacgtc ggcgtgctgg cagctatgat tatggcgagc ctttctggtt
    17161 ctgccgttgc ggataccgcc gccgttgccg ctctactggt gccgatgatg cgcagcgcca
    17221 actacccggt caaccgggcg gcggggctga ttgcctctgg cggcattatc gcgccaatta
    17281 ttcccccttc cattccgttt attatcttcg gcgtttccag cggattatcc atcagtaagc
    17341 tgtttatggc gggcattgcc cctggcatga tgatgggcgc aacgctgatg cttacctggt
    17401 ggtggcaggc cagtcgcctt aatttacccc gccagcaaaa agcaacgatg caggaaatct
    17461 ggcactcttt tgtctccggt atctgggcgc tgtttcttcc agtcatcatt attggcgggt
    17521 ttcgctccgg tctgtttacc cctaccgaag ccggggccgt tgctgctttc tatgcgctgt
    17581 ttgtcgccac agttatttac cgtgaaatga ccttcgccac actttggcat gtactcattg
    17641 gcgcggcgaa aaccacctca gtggttatgt ttctggtggc ctcggcacaa gtttccgcct
    17701 ggctgattac cattgctgaa ctgccgatga tggtttctga tttactgcaa ccgttggtcg
    17761 attcaccgcg tctgctgttt atcgtcatta tggtggctat cctgattgtc ggcatggtca
    17821 tggatttaac gccgacggta ttaattctta ccccggtatt aatgccttta gttaaagagg
    17881 caggaatcga tccgatttat ttcggtgtca tgtttatcat taactgctca atcggcttaa
    17941 ttacaccgcc tatcggtaat gtacttaacg ttatttcagg ggtggcaaaa ctcaaattcg
    18001 atgatgcagt cagaggcgtt ttcccttacg tcctggtttt atattcatta ttagtcgtat
    18061 ttgtttttat tcccgatctc atcatcctcc ctttgaaatg gattaattaa aaggaaaata
    18121 ttatgaaatt acgctctgta acctacgcat tattcattgc tggcctggct gcattcagca
    18181 catcttctct ggcggcgcaa tctttacgtt tcggttatga cacctcacaa accgactcgc
    18241 aacatattgc ggcgaaaaaa ttcaatgatt tattgcagga gagaaccaaa ggcgagctga
    18301 aattaaaatt gtttccggat agcaccctcg gtaacgcgca ggcgatgatc agcggcgtac
    18361 gtggcggcac catcgatatg gaaatgtccg gctcgaataa ctttgccggg ttatcaccag
    18421 tgatgaactt gcttgatgtc cctttcctgt tccgcgatac cgctcacgcg cataaaacgc
    18481 tcgacggcaa agtcggtgat gatctgaaag cctcacttga aggtaaagga ctaaaagtac
    18541 tggcctactg ggaaaacggc tggcgcgatg tcaccaactc gcgcgcaccg gttaaaaccc
    18601 acgccgacct gaaagggctg aaaattcgca ccaacaatag cccgatgaat atcgccgcat
    18661 tcaaagtctt tggcgctaac ccgatcccga tgccgtttgc cgaagtctat accgggctgg
    18721 aaacccgcac tatcgacgct caggaacacc cgatcaacgt cgtctggtca gcaaaatttt
    18781 tcgaagtgca gaagtacctt tctctgacgc accacgccta ttccccgctt ctggtggtga
    18841 tcaacaaagc gaagtttgat ggcttaagcc cggagttcca gcaagcgcta atttcatctg
    18901 cgcaggaagc gggtaactac cagcgcaaac tggttgccga agatcagcaa aaaatcatcg
    18961 acggcatgaa agaagcgggc gtggaagtca tcaccgatct cgaccgcaaa gcctttagcg
    19021 acgctctggg taatcaggtt cgcgacatgt ttgtaaaaga tgttccgcag ggtgctgatc
    19081 tactgaaagc cgtggatgag gtgcaataaa ccatgacgca atactggctg gggttagatt
    19141 gtggcggtag ctggctgaaa gccgggctgt atgaccgcga aggccgggag gcaggcgtgc
    19201 agcgcctgcc gctgtgcgca ttaagcccgc agccaggttg ggcagagcgc gatatggcag
    19261 aactgtggca atgctgcatg gctgtcattc gcgaactgct tacccattcc ggcgttagcg
    19321 gggaacaaat tgtcggtatc ggtatctccg cacaaggaaa aggcttgttt ttgctggata
    19381 aaaacaacaa gccgctcggt aacgccattt tatcctcgga tcgccgggcg atggaaatcg
    19441 ttcgtcgctg gcaggaagat ggcatcccgg aaaaactcta cccgctgacc cgacaaacct
    19501 tgtggaccgg gcatccggta tcgctgttac gttggcttaa agagcacgaa ccggaacgct
    19561 acgcgcaaat tggctgcgtg atgatgacgc acgactacct gcgctggtgt ttaaccggcg
    19621 tcaaaggctg tgaagagagc aatatttccg agtccaatct ctacaacatg agtcttgggg
    19681 aatatgaccc gtgcctcacc gactggctgg ggattgctga aatcaatcac gccctgccgc
    19741 ctgttgtcgg atctgccgaa atctgcgggg agatcaccgc tcagacagcc gtactgaccg
    19801 gtctgaaagc gggtacgccc gttgtcggcg gcctgtttga tgtggtttcc accgcactct
    19861 gcgccgggat cgaagacgaa tttaccctta atgcggtgat gggcacctgg gcggtgacca
    19921 gcggcataac ccgcggttta cgtgacggtg aagcgcatcc gtatgtctat ggtcgctacg
    19981 ttaacgatgg tgaatttatc gttcacgaag ccagccctac ctcttccggc aacctcgaat
    20041 ggtttaccgc acagtgggga gaaatctcgt ttgatgagat caaccaggcc gttgccagct
    20101 tgccgaaggc cggtggcgat ctctttttcc tgccgttcct gtacggcagc aacgccggac
    20161 tcgagatgac cagcggtttc tacgggatgc aggccattca cacccgcgca cacctgttgc
    20221 aggccattta tgaaggggtg gtgttcagcc atatgaccca cctcaaccgg atgcgcgaac
    20281 gttttactga tgtgcacacc ctgcgcgtca ctggcggccc ggcgcactcc gatgtctgga
    20341 tgcaaatgct ggcggacgtc agcggtctgc gtatcgagct gccgcaggtg gaagaaaccg
    20401 gctgttttgg cgcggcactt gccgcccgtg tcggcaccgg agtttatcgc gatttcagcg
    20461 aagctcaacg tgacttacag cacccggtac gcaccctgct gccggatatg accgcccatc
    20521 agctttacca acaaaaatac caacgctatc agcatctcat tgccgcactt cagggctttc
    20581 acgcccgcat caaggagcac atattatgag ccgaccactt ctgcaactgg cactcgacca
    20641 ctcatcactt gaagccgcac agcgcgacgt gacgcagtta aaagacagcg tcgatatcgt
    20701 cgaagcgggc accattctct gtttaaacga agggcttggc gcggtgaaag ccttgcgcga
    20761 acaatgcccg gacaaaatca tcgttgccga ctggaaggtc gccgacgctg gtgaaacgct
    20821 cgcgcaacag gcatttggcg caggcgctaa ctggatgacc atcatctgcg ccgcgccgct
    20881 cgcgacggta gaaaaaggcc acgcaatggc gcaacgctgc gggggtgaaa ttcagataga
    20941 gctgttcggt aactggacgc tggacgacgc ccgcgactgg catcgtattg gcgtgcggca
    21001 ggccatttat catcgcggtc gtgatgcaca ggccagcggg caacagtggg gcgaagccga
    21061 tctggcacgc atgaaggcgc tttctgatat cggtcttgaa ctttccatta ccggcgggat
    21121 tacccctgct gacctgccgc tgtttaaaga tatccgcgtg aaagcgttta ttgccgggcg
    21181 ggcgctggca ggcgcagcaa atccggcgca ggtggctggc gatttccatg cgcaaatcga
    21241 cgctatctgg ggaggcaagc gtgcgtaatc atccgttagg gatttatgaa aaggcgctgg
    21301 cgaaagatct ctcctggccg gagcggctgg tgctggcaaa aagctgcggt tttgattttg
    21361 tcgaaatgtc ggtggatgaa accgacgaac ggctttcacg tcttgactgg agcaccgcac
    21421 aaaggacttc gctggttgcc gcgatgatcg aaacgggtgt tgggatcccg tcgatgtgcc
    21481 tgtccgcaca tcgtcgcttt ccttttggta gccgtgacga cgcggtacgc cagcgagcac
    21541 gggagatcat gagcaaagcg attcgtctgg cacgcgatct cggcatacgc accatccagc
    21601 tggcgggcta cgacgtctat tacgaagacc acgatgaagg cacccggcaa cgttttgctg
    21661 aagggctggc gtgggcggtc gaacaggcgg cggcatcaca agtcatgcta gcggtggaga
    21721 ttatggacac cgcgtttatg aactccatca gtaaatggaa aaagtgggac gagatgctcg
    21781 cctcgccgtg gttcaccgtc tacccggacg tcggcaacct cagcgcctgg ggcaatgatg
    21841 ttcccgccga actgaaactg ggcattgacc gtatcgccgc gatccacctg aaagacaccc
    21901 agccagttac cgagcaaagt cccggacagt tccgcgacgt gccgtttggc gaaggctgcg
    21961 tcgatttcgt tggcatcttc aaaacgctgc ataaactgaa ctatcgcggt tctttcctga
    22021 ttgagatgtg gaccgaaaaa gccaaagagc cggtgctgga gattattcag gcgcggcgtt
    22081 ggattgaagc gcgtatgcag gaggctggat ttatatgtta gagcaactga aagccgacgt
    22141 gctggcggcg aatctggcgc ttcccgctca ccatctggtg acgttcacct ggggcaatgt
    22201 cagcgcggta gacgaaacgc ggcaatggat ggtaatcaaa ccttccggcg tcgagtatga
    22261 cgtgatgacc gccgacgata tggtggtggt tgagatagcc agcggcaagg tggtggaagg
    22321 tagcaaaaag ccctcgtccg acacgccaac gcatctggcg ctttaccgtc gctacgccga
    22381 aatcggtggc attgtgcata cccactcacg ccacgccacc atctggtcgc aggccgggct
    22441 ggatctcccc gcctggggca ccacgcacgc tgattatttc tatggtgcca tcccctgcac
    22501 gcgacagatg accgcagagg agattaacgg cgaatatgaa tatcagaccg gcgaagtgat
    22561 cattgaaacc ttcgaagaac gtggcaggag tccggcacaa atcccggcgg tgctggtgca
    22621 ttctcacggc ccgttcgcat ggggtaaaaa cgccgccgat gccgtgcata acgccgtagt
    22681 actcgaagaa tgcgcctata tgggcctatt ctcgcgccag cttgcgccgc agctccctgc
    22741 gatgcaaaac gaactgctgg ataagcacta cctgcgtaag catggggcca atgcctatta
    22801 cgggcagtaa tccctcacgc cggggcttca tcgccccggc actacgaatt gatatgttcc
    22861 ttgctgtaac gccgcttcca cgctgctggc gttaaaccgg tgtgcttgcg aaaaatctgc
    22921 cggaaatagc caacgtcatt aaaccgtatt gcatggagca ggccgacaac gtcgagtcac
    22981 tgcataacct gttgctgccc gccgatgcgc catttacgac atttgaaggc aagggattgt
    23041 tcagccataa gatcctgatt cagacgccag gttcccggta tgaacagagc aatccagagc
    23101 aacaaccttt gtggcattac gacggagcac cagccgcacc gacatccacc ggtgaattaa
    23161 aaacgccagc tcacgccggt gataaaggta aaactctcat tacgatcaat catcgggctg
    23221 ttggtaatct cttccggcag ctcgctgtat cctgcgctcg ccatcaatac gacgtgctct
    23281 cctatcgggt attttgccgt caggctgaca tagggcaccc aggcatcctg cgcggaataa
    23341 cttgccagac cgctacgacg ggactcactc tctgaaatgc catagtaata gtcactgaaa
    23401 ttctcgtcat aatagagaac gcccagcgca ggtgtcagcg acaggcgacc tatctgcatt
    23461 ttgtggaata ccgatagctc ccccacccag ccgttgctgt tatccagaac gtccgcagcc
    23521 gcagaggctt tcacggttcc ccaacgctcg tggtgatacc aggcaacccc cgccatagcc
    23581 gtactatcac gcttatcaag ctgttgcatg gcataatcgt cattatcggc agggtcaaat
    23641 tccagcggca tccaggatgc ggtcaggcta agttcatttt tttcactttg cgacagaata
    23701 aaacctaatg tggtctgacg aacataaaaa gaatcacctt cataactaat cagcggaatt
    23761 gcctttgtat tttcattata accgcgataa ggcgattcat tataagctgc accagcacca
    23821 atggataatt cagaagcagt tgcgcttgcc ataaaaggca acgcaaataa cgccacaata
    23881 ttgcgattaa ttaacatgtc cggtattcca ttttaaaata agataaaaaa gagtcggcat
    23941 agtaatgctt acaatttctt cggtgcaaga ttcatattta tatagtaata tataaaatta
    24001 tatataattg ggctgttgcg atgacaaaat tacaacttaa gtaccgggag ttaaaaatta
    24061 tctcggtaat cgctgccagt gaaaatatca gccatgccgc gactgtactt ggcatcgcac
    24121 aggccaacgt cagcaaatac cttgctgatt ttgaatcaaa agtgggttta aaagtctttg
    24181 accgaaccac ccggcagctg atgctcacac cttttggtac cgcgctgtta ccgtacatca
    24241 acgacatgct ggacagaaac gagcagctta ataattttat tgcagattat aagcatgaga
    24301 aacgtggtcg ggtcaccatc tacgccccaa ccggcataat tacctattta tccaaacatg
    24361 taatcgataa aattaaagat atcggtgata tcaccttatc gctaaaaacc tgcaatcttg
    24421 agcgtaatgc cttttatgaa ggcgtcgaat ttcctgatga ttgtgatgtt ttaatcagct
    24481 acgctccacc aaaagatgaa tcgctggttg ccagttttat tacccaatat gccgtaaccg
    24541 cttatgccag ccagcgctat cttgagaaac atcccattag ccgtcctgac gaactcgaac
    24601 atcactcctg tattttgatc gactcgatga tgatcgacga cgcgaatatc tggcgcttca
    24661 acgtcgccgg gagtaaagag gtgcgggatt atcgcgtgaa gggaaattac gtttgcgaca
    24721 acacgcaatc tgcgctggag ctggcacgaa atcacctggg gattgtgttt gcgccagaca
    24781 aaagtgtgca gagcgacctg caagacggca cgctggtgcc ctgctttcag cacccgtatg
    24841 aatggtggct ggatctggtg gctatcttcc gtaaacggga ataccagccc tggcgggtcc
    24901 agtatgtgct ggatgaaatg ctgcgtgaaa ttcgccacca gcttgctcag tcgcagcaac
    24961 tgcggcctga acaagcggcg gaaagcgaag attaatgatc gagataaagg taatgcaccc
    25021 agctggtcat gcgtaatagc actttgcgca tcaccgagac atggatgaaa tgatcagagt
    25081 agaccgccac ctgggcgtaa ataccgtctg gtaaagcgct caaatcagtg tgctcatcca
    25141 gttcaatggt cgcgataacg ccatctgaac ccggcgctgt gtttaacgtc tgtaaggtgc
    25201 cggtcgactg ataagctccg ccgggaacag ctggactaat ggctgccagc ttgccgctga
    25261 ataccttacc tggcagagca ttaaacacca cttccgcatc gtcgccaggg gccagtcgca
    25321 gcaaggagtt ctgacggaac tgcgccacaa tttgtcgttt ctgatcgggt ataaacacca
    25381 tcaccggacg tagcggcagc gacgcggcat aggtacccgg acgaatcagc acctgggtca
    25441 cataaccatc gctcggcgca cgcactatcg tctgctcaag gttatatttt gcttccgcga
    25501 gctgcgcttt caggctggcg atttgagaat gttcacccaa caccaggcta tccagctggc
    25561 tctggatctg tttttgttcc gccgccgatg actttacgga ggcttcctgc gccagataat
    25621 tttgccgcgc cacatcaata tcgcgttctg aaaacgggtt tactttcgcc tggctgccgc
    25681 gtgcgtaacg ctgatactct ttagcgaatt tatcccgcgt ggcctttgcc tgctgagtat
    25741 tcgccgccat ctcatctaac tctgcgccca acgcccgctg tttatgttct gcggtaacga
    25801 tatccgccat cagccgatcc acccgcgcct gataacgcgt cgggtccagt cgaaatagca
    25861 cctcaccttt tttaatcagc gtatttttct tatccgtcac ttcgatcacc acgcctgtca
    25921 cctgtgggac aacaggaata gaaataaccg ctttttgcgc tttgaaggta tacggatggt
    25981 tatagttcat taaggctttg ttgaataaat cagatttcgg gtaagtctcc cccgtagcgg
    26041 gttgtgtttt caggcaatac gcacgctttc aggcatacct gctttcgtca ttttgttcag
    26101 cgctcgtacc agggccatag cctccgcaac ctgaccatcg tagtcacgca gcgtcagtga
    26161 acccccgaac agctgtttta cccggtacat cgccgtttcc gctatcgagc gacggttgta
    26221 atctgttgtc catttccacc gcgcattact cccggtcatt cgctgattag ccactgcacg
    26281 gttacggtct gcatattcac cgggccagta acccgcacct tttcggggag ggataagcgc
    26341 gctgattttc ttacgccgca gttcatcgtg acatagccgg gtatcgtaag cgccatcggc
    26401 ggcggctgac ctgattttcc ggtgggtttg ccggattaac ccggggaagg cctctgagtc
    26461 cgtaacgttg ttcagcgaca ggtcagcgca gatgatttca tgtgttttac tgtcaacggc
    26521 gagatgcagc ttacgccaga tacggcggcg ttcctggcca tgctttttga ctttccactc
    26581 gccttcaccg aagaccttca gcccggtgga atcaattacc aggtgtgcga tttcaccccg
    26641 ggtgggcgtt ttgaaactga cattaaccga ctttgcccgc ctgctgacac agctgtaatc
    26701 cgggcagcgt agcggaacgt tcatcagaga aaaaatggaa tcaataaagc cctgcgcagc
    26761 ccgcagggtc agcctgaata cgcgtttaat gaccagcaca gtagtgatgg caaggtcaga
    26821 atagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatagc
    26881 ttcatcatcc agccagaaag ttatggagcc acggttgatg agggctttat tgtaggtggg
    26941 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagata
    27001 cgtgatctga tccttcaact cagcaaaag
//
LOCUS       DAMZII010000063        26919 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZII010000063 DAMZII010000000
VERSION     DAMZII010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.063"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            11..757
                     /gene="ycaL"
                     /locus_tag="POA77_002558"
     CDS             11..757
                     /gene="ycaL"
                     /locus_tag="POA77_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDI6365963.1"
                     /translation="MLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAK
                     AIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            930..1613
                     /gene="cmk"
                     /locus_tag="POA77_002559"
     CDS             930..1613
                     /gene="cmk"
                     /locus_tag="POA77_002559"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI6365964.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1724..3397
                     /gene="rpsA"
                     /locus_tag="POA77_002560"
     CDS             1724..3397
                     /gene="rpsA"
                     /locus_tag="POA77_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI6365965.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3557..3841
                     /gene="ihfB"
                     /locus_tag="POA77_002561"
     CDS             3557..3841
                     /gene="ihfB"
                     /locus_tag="POA77_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDI6365966.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4048..6312
                     /locus_tag="POA77_002562"
     CDS             4048..6312
                     /locus_tag="POA77_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDI6365967.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6349..8097
                     /gene="msbA"
                     /locus_tag="POA77_002563"
     CDS             6349..8097
                     /gene="msbA"
                     /locus_tag="POA77_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDI6365968.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8094..9080
                     /gene="lpxK"
                     /locus_tag="POA77_002564"
     CDS             8094..9080
                     /gene="lpxK"
                     /locus_tag="POA77_002564"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDI6365969.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9117..10349
                     /locus_tag="POA77_002565"
     CDS             9117..10349
                     /locus_tag="POA77_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDI6365970.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10401..10583
                     /gene="ycaR"
                     /locus_tag="POA77_002566"
     CDS             10401..10583
                     /gene="ycaR"
                     /locus_tag="POA77_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDI6365971.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10580..11326
                     /gene="kdsB"
                     /locus_tag="POA77_002567"
     CDS             10580..11326
                     /gene="kdsB"
                     /locus_tag="POA77_002567"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDI6365972.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11480..12373
                     /locus_tag="POA77_002568"
     CDS             11480..12373
                     /locus_tag="POA77_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDI6365973.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12350..13129)
                     /gene="elyC"
                     /locus_tag="POA77_002569"
     CDS             complement(12350..13129)
                     /gene="elyC"
                     /locus_tag="POA77_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDI6365974.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13265..14050
                     /gene="cmoM"
                     /locus_tag="POA77_002570"
     CDS             13265..14050
                     /gene="cmoM"
                     /locus_tag="POA77_002570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDI6365975.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14047..15369
                     /gene="mukF"
                     /locus_tag="POA77_002571"
     CDS             14047..15369
                     /gene="mukF"
                     /locus_tag="POA77_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDI6365976.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15350..16054
                     /gene="mukE"
                     /locus_tag="POA77_002572"
     CDS             15350..16054
                     /gene="mukE"
                     /locus_tag="POA77_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDI6365977.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16054..20514
                     /gene="mukB"
                     /locus_tag="POA77_002573"
     CDS             16054..20514
                     /gene="mukB"
                     /locus_tag="POA77_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDI6365978.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20775..22622
                     /gene="ldtD"
                     /locus_tag="POA77_002574"
     CDS             20775..22622
                     /gene="ldtD"
                     /locus_tag="POA77_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6365979.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22803..23351
                     /locus_tag="POA77_002575"
     CDS             22803..23351
                     /locus_tag="POA77_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDI6365980.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23378..24025
                     /gene="gloC"
                     /locus_tag="POA77_002576"
     CDS             23378..24025
                     /gene="gloC"
                     /locus_tag="POA77_002576"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDI6365981.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24247..25437)
                     /gene="aspC"
                     /locus_tag="POA77_002577"
     CDS             complement(24247..25437)
                     /gene="aspC"
                     /locus_tag="POA77_002577"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDI6365982.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25622..26710)
                     /gene="ompF"
                     /locus_tag="POA77_002578"
     CDS             complement(25622..26710)
                     /gene="ompF"
                     /locus_tag="POA77_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDI6365983.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6517 a         6589 c         7309 g         6504 t
ORIGIN      
        1 tactaaatta ctgctggcga ttgcgacctc tgcagcatta ctgacagggt gtcaaaatac
       61 ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata aagcagcaac
      121 attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa tggacagcgg
      181 caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa tcgccaaagc
      241 attgggtaac aatattaatg gcacgccggt caactataag gtttatatga ccagcgacgt
      301 caacgcatgg gcgatggcga acggctgtgt ccgtgtctac agtggcctga tggacatgat
      361 gaatgacaac gaaattgaag gtgttctggg ccatgaactg ggccacgtcg cgttgggtca
      421 ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg ccatttcagc
      481 taccagcgga gtggcttccc agctttcccg ctcacaattg ggtgatatcg cagaaggcgc
      541 tattaatgct aaatactccc gtgataaaga gtccgaagca gatgatttct cctttgatct
      601 gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa aactggctag
      661 tctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa cagagcgtgc
      721 gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct ttcgggctgg
      781 tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag acaaaactgg
      841 tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg cggttatgtt
      901 aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt attaccattg
      961 atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa gcattgcaat
     1021 ggcatctgct ggattcgggt gcaatttatc gcgtactggc attggcggca ttacatcacc
     1081 atgttgatgt tgcgtcagaa gatgcgctgg taccgctggc atcccatctg gatgtacgtt
     1141 ttgtgtcaac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc agcggcgaaa
     1201 ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca cgcgttcgtg
     1261 aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggcctgatt gccgatggcc
     1321 gcgacatggg aacggtggta ttccctgatg cgccagtgaa aattttcctt gacgcctcct
     1381 cggaagaacg agcgcatcgc cgcatgctac agttgcagga gaagggcttt agtgttaact
     1441 ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac cgagcggtag
     1501 cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta agcattgagc
     1561 aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca taagcgaccg
     1621 aatttgcagt acccccgttg caatggaatg gcagcgggta tgttaaacaa ccccatccgg
     1681 catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg aatcttttgc
     1741 tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta tcgttcgtgg
     1801 cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga aatctgagtc
     1861 cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc aggtaggtga
     1921 cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc tgctgtcccg
     1981 tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg aagatgctga
     2041 aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg agctgaacgg
     2101 tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg acactctgca
     2161 cctggaaggc aaagagcttg aattcaaagt aatcaagctg gatcagaagc gcaacaacgt
     2221 tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg atcagctgct
     2281 ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca ctgactacgg
     2341 tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca tggcctggaa
     2401 acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg ttaaagtgct
     2461 gaagttcgac cgcgaacgta cccgtgtatc tctgggcctg aaacagctgg gcgaagatcc
     2521 gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc gcgtgaccaa
     2581 cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc tggtacacgt
     2641 ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta acgttggcga
     2701 tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct ccctgggtct
     2761 gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca agggcgaccg
     2821 tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc tggacggcgg
     2881 catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg aagaagcagt
     2941 tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg acgcagaacg
     3001 tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca actgggttgc
     3061 tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg ctaaaggcgc
     3121 aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag catcccgtga
     3181 ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag ctaaattcac
     3241 cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag acgaagctga
     3301 cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct ccaacaacgc
     3361 aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt cgggattttt
     3421 attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta atcaagcact
     3481 aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa tttgccttta
     3541 aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca cccagcaatc
     3601 gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc atatggcctc
     3661 gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct ctttgcacta
     3721 ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac tggaaggaaa
     3781 atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata tttacggtta
     3841 agtttttact caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt tcatttctct
     3901 aatcaggaac tggaagctgc ctcgcagagt tttgagcagt tttcaccctt tcgttaaatt
     3961 cttctgaatg tgcctcgggg aacgcaaaat tcccaccaga caaccgctca acaaagttgc
     4021 acactttcca taaacaggga ggggtgcatg aaaataacga cagtcggcgt atgcataatt
     4081 tgcggaattt ttccgttgct gattttgccc caattgcctg ggacattaac ccttgcgttt
     4141 ctgactctct tcgcctgcgt actggcattt atccctgtta aaaccgtccg ttatatcgcg
     4201 ctgacgttgc tgtttttcgt ttggggcata ttatcagcaa agcaaatttt gtgggcagga
     4261 gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcactgcaac tgacggcatg
     4321 accactcatt acggtcaaat tactcatcta caaggtcgac gtatattccc tgcgccaggc
     4381 cttgtgctgt atggcgaata tcttccgcaa gcggtttgtg ctggacaaca atggtcaatg
     4441 aaactcaaag ttcgtgcggt tcatggccaa cttaatgatg gcggctttga tagccagcgt
     4501 tatgccattg cccagcatca accgctcacc ggccgctttc tgcaggcaag tgttattgaa
     4561 ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct gcaaccctat
     4621 ccgtggaatg cggttattct tggtttaggt atgggggaac ggttatccgt ccccaaagaa
     4681 atcaaaaata tcatgcgcga tactggaacg gcgcatttaa tggcgatatc gggattgcac
     4741 atcgcttttg cggcgttgct ggctgccgga ctcattcgca gtggacaaat ttttctgcct
     4801 gggcgctgga tccactggca aataccatta attggtggga tctgctgtgc tgctttttat
     4861 gcctggttga cgggaatgca acctcctgca ttgcgtaccg tggtggcgct tgctacgtgg
     4921 ggaatgctta agttaagtgg gcgacagtgg agtggctggg atgtatggat atgttgtctg
     4981 gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg gctctctgcc
     5041 gctgcggtcg cggcactgat tttttggtat cagtggtttc cctgtcctga gtggcaactg
     5101 ccgccggtat tgcgtgcagt tgtttccctc atccatctgc aactgggtat cacacttctg
     5161 cttatgcccg tgcaaatcgt catatttcat ggcattagtc tgacctcgtt tattgcaaat
     5221 ctattagcaa ttcccttggt gacatttatc acggttccgt tgatcctcgc cgcgatggtt
     5281 gtgcatttaa gcgggccgtt aatcctggag caagggttat ggtttcttgc cgaccggtct
     5341 ttggctttac ttttctgggg gttaaagagt ttgccagaag ggtggatcaa cattgctgaa
     5401 cgttggcaat ggctatcatt ttccccatgg ttcttactgg tggtatggcg attaaacgcc
     5461 tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctg gccgctatgg
     5521 caaaaacctc gacctgacga gtggcaggtg tacatgcttg atgtcgggca agggctggca
     5581 atggtgatag ccagaaacgg caaagcgatt ctctatgaca caggactggc ctggcccgaa
     5641 ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct tgaaccggaa
     5701 ggcgttattc tgagtcatga acatctggat caccggggag ggctggactc aatattgcac
     5761 acatggccga tgttatggat cagaagtccg ttaaactggg aacatcatca gccctgtgtg
     5821 cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg tgcactggcc tttacaagct
     5881 agcaacgata aaggaaataa ccattcctgt gtggttaagg ttgatgacgg gacgaatagc
     5941 attcttctaa ccggtgatat tgaagtcccc gctgaacaaa agatgctaag ccgttactgg
     6001 cagcaagtgc agacaacatt gcttcaggta cctcaccatg gcagtaatac ctcatcatcg
     6061 ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc gcgctataac
     6121 gcatggcgat tgccctctaa taaagttaag catcgctatc aacagcaagg atatcaatgg
     6181 cttgatactc cacatcaggg tcaagtgacg gtcaattttt cagcgcaagg ctggcggatt
     6241 agcagcctca gagagcaaat tttacctcgt tggtatcatc agtggtttgg cgtgccagtg
     6301 gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat gcataacgac
     6361 aaagatctct ctacgtggca gacgttccgc cgactgtggc caaccattgc gccttttaaa
     6421 gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga taccttcatg
     6481 ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg ctccgtgctg
     6541 gtgtggatgc cgctggtggt gatcgggctg atgattttac gtggtatcac cagctatgtc
     6601 tccagctact gtatctcctg ggtatcagga aaggtggtaa tgaccatgcg tcgccgcctg
     6661 tttggtcaca tgatgggaat gccggtttca ttttttgaca aacagtcaac ggggacgctg
     6721 ttgtcacgta tcacctacga ttccgaacag gttgcttctt cttcttccgg cgcactgata
     6781 acggttgtgc gtgaaggtgc gtcaatcatc ggcctgttca tcatgatgtt ctattacagt
     6841 tggcaactgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc gattcgcgta
     6901 gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg gcaggtgacc
     6961 accagcgccg aacaaatgct gaagggccac aaagaagtat tgattttcgg tggtcaggaa
     7021 gtggaaacga aacgctttga taaagtcagc aaccgaatgc gtcttcaggg gatgaaaatg
     7081 gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt ggcgctggcg
     7141 tttgttctgt atgcggcgag cttcccaagt gtcatggata gcctgactgc cggtacgatt
     7201 accgttgttt tctcttcaat gattgcactg atgcgtccgc tgaaatcact gaccaacgtt
     7261 aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat tctggacagt
     7321 gagcaggaga aagacgaagg taagcgcgtg atcgagcgtg cgactggcga cgtggaattc
     7381 cgcaatgtca cctttactta tccgggacgt gacgtacctg cattgcgtaa catcaacctg
     7441 aaaattccgg cagggaagac ggttgctctg gttggacgct ctggttcagg taaatcaacc
     7501 atcgccagcc tgatcactcg tttttacgat attgatgaag gcgaaatcct gatggatggt
     7561 cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct ggtgtcgcag
     7621 aatgtccatc tgtttaacga tacggttgct aacaacattg cttacgcacg gactgaacag
     7681 tacagccgtg agcaaattga agaagcggcg cgtatggcct acgccatgga cttcatcaat
     7741 aagatggata acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct ctctggcggt
     7801 cagcgtcagc gtattgctat cgctcgagcc ttgctgcgtg atagcccgat tctgattctg
     7861 gatgaagcta cctcggctct ggataccgaa tccgaacgtg cgattcaggc ggcactggat
     7921 gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac cattgaaaag
     7981 gcagacgaaa tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac gcataacgat
     8041 ttgcttgagc accgcggcgt ttacgcgcaa cttcacaaaa tgcagtttgg ccaatgatcg
     8101 aaaaaatctg gtctggtgaa tcccctttgt ggcggctatt gctgccactc tcctggttgt
     8161 atggcctggt aagtggcgcg atccgtcttt gctataaact aaaactgaag cgcgcctggc
     8221 gtgcccccgt accggttgtc gtggttggta atctcaccgc aggcggcaac ggaaaaaccc
     8281 cggtcgttgt ctggctggtg gaacagttgc aacagcgcgg tattcgcgtg ggggtcgtat
     8341 cgcggggata tggtggtaag gctgaatctt atccgctgtt attgtcggca gataccacaa
     8401 cagcacaggc gggtgatgaa cctgtgttga tttatcaacg cactgatgcg cctgttgcgg
     8461 tttctcccgt tcgttctgat gcggtaaaag ccattctggc gcaacaccct gatgtgcaga
     8521 tcatcgtaac cgacgacggt ttacagcatt accgtctggc gcgtgatgtg gaaattgtcg
     8581 ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg ccaatgcgtg
     8641 agcgagcggg gcgcttaaag tcggttgatg cggtaatcgt caacggcggt gtccctcgca
     8701 gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt accggtacgc
     8761 gttgtgacgt tgctcagctt gaacatgtag tggcgatggc ggggattggg catccgccgc
     8821 gcttttttgc cacgctgaag atgtgtggcg tacaaccgga aaaatgtgta ccgctggccg
     8881 atcatcagtc tttgaaccat gcggatgtca gtgcgttggt aagcgccggg caaacgctgg
     8941 taatgactga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat tggtggtatt
     9001 tgcctgtaga cgcacagctt tcaggtgatg aaccagcgaa actgcttacg caactaacct
     9061 cgctggcttc tggcaactag ttacgccgcg gcagcgttcg attgatggag tcataaatgt
     9121 cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgca caaggcctgt
     9181 taaacaaacc ccgccgtcga gcgtcgttgg aggatattcc ggcaacgatc tcccgcatgt
     9241 ccttgctgca aatcgatacc atcaatattg ttgcccgtag tccatatctg gtgcttttca
     9301 gtcgtctggg aaattatcct gcccagtggc tggatgagtc tctggcgcgt ggcgaattaa
     9361 tggaatactg ggcgcatgaa gcctgcttta tgccgcgtag cgactttcgt cttattcgcc
     9421 accgcatgct ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg atgcaggaac
     9481 atgaggcgga aattgcacag ttaattcagc atattcatga taaggggccg gtacgttcag
     9541 ccgattttga gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag ccgcataaac
     9601 ggcatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg cgcaacttcc
     9661 agcgcgttta tgatttaacc caccgtgtca tgcctgactg ggatgatgag cgcgatctcg
     9721 tttcgcaaac agaagcagaa atcatcatgc tggataacag tgcgcgtagc ctgggaatat
     9781 tccgcgaaca gtggctggca gattactatc ggctgaaacg tccggcactg gcggcgtggc
     9841 gcgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa ttgggcaatc
     9901 tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctggccgga aagctcactg
     9961 caacgcacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc aaacgcgcag
    10021 agcagctttt tgattttagc taccggctgg agtgctatac cccagcgccg aaacgccagt
    10081 atggctattt tgttctgcca ttattacatc gtgggcaatt agttgggcga atggatgcca
    10141 aaatgcatcg ccagacaggc atccttgaag ttatctctct gtggttacag gaaggcatta
    10201 aaccaacgac aatgctgcaa aaagggttac gtcaggcgat tactgatttc gcgagctggc
    10261 agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg tctctttact gattgccgca
    10321 ctggctggga aatagacccc gtcgcataaa tgattatgat aagatttatg cattcataac
    10381 ccgatccatc tggaggaact atggatcatc gtctgcttga aatcattgcc tgcccggttt
    10441 gcaacggaaa actttggtat aaccaagaaa aacaagaact tatttgcaaa ctggacaacc
    10501 tggctttccc gctgcgtgat ggcattccag tgttgctgga aacggaagcc cgcgtgctga
    10561 ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgct acgcgtcaac
    10621 gcgtctgccc ggtaaaccat tggttgatat taatggcaaa cccatgattg ttcatgttct
    10681 tgaacgtgct cgcgaatcag gtgccgatcg catcatcgtg gcaaccgatc atgaggatgt
    10741 cgcccgcgcc gttgaagccg ctggcggtga agtatgtatg acgcgcgccg atcaccagtc
    10801 agggacagaa cgcctggcgg aagttgtcga aaaatgcgca ttcagcgacg acacggtgat
    10861 cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc aggttgctga
    10921 taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc acaatgcgga
    10981 agaagcgttt aacccgaatg cggtgaaagt ggttctcgac gctgaagggt atgcactgta
    11041 cttctctcgc gccaccattc cttgggatcg tgatcgtttt gcagaaggcc ttgaaaccgt
    11101 tggcgataac ttcctgcgtc atcttggtat ttatggctac cgtgcaggct ttatccgtcg
    11161 ttacgtcaac tggcaggcaa gtccgttaga acacatcgaa atgttagagc agcttcgtgt
    11221 tctgtggtac ggcgaaaaaa tccatgttgc tgttgctcag gaagttcctg gcacaggtgt
    11281 ggatacccct gaagatcttg agcgcgttcg cgctgaaatg cgctaatttc acttcacgac
    11341 acttcagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat ttttcttttc
    11401 aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga aagcagtagc
    11461 ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt tactgggcga
    11521 aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt gggctttgta
    11581 tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc ccggaaaagc
    11641 ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc gtatgccgac
    11701 tatttatggt gtgatgacgc atgaagaaca ccccggcccc gatgtcctgc tactggagcg
    11761 gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg aacaactcaa
    11821 agaccaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg gttgcgtcgg
    11881 cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac atgttgaagt
    11941 gctatggacc acgctcaatc agttcaataa caccggtctg acgatgcagg ataagcggat
    12001 cctgtttcgc actcgcgaat gtctcccggc attatttgaa ggctttaacg acaattgtgt
    12061 gttgattcac ggtaacttct gtttacgcag catgctgaaa gattcgcgca gcgatcagtt
    12121 actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg aactgttccg
    12181 actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac gcgcgccagt
    12241 ggcggagtcg ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag ttgcgcaact
    12301 ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat cactcttgcc
    12361 gtggctcgcc tgacggacct ttcagccact gccagatgcg gccaagcgtt tcatagccaa
    12421 cacgatcgct atgcatcagc cagaccggag aagggattgc ccgttcccac ggattaagcg
    12481 gcgagtcgat ggccaactga ttcgccgggg ctggtagcgg atttaaccct tcctgctgaa
    12541 aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa ggggcgtcac
    12601 caatcgcctg tttcactgcc gcagcttctt cttcggtatc ttttggcaaa tccagggtga
    12661 taatttgctc gcgcggcacg cccagcgatt gcgcaactct ggcacctact tccgctgtac
    12721 tcaccgtatt ggtttttgct acgcctcccg tgaagatcag ttttgatccc ggattttcgc
    12781 gccataagcg aataccttca ttcaggcgtg gcagactgtt attgattaaa ttagagctcg
    12841 gtgcccactg tgggttccag gtataaccac ctccgagcac cacgatatag tccacttttt
    12901 gcgaattatt ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg tcggctaccg
    12961 gttgcaaact caataacaag agtgccagcc acccaatgct aataaaaatc ttcccggttt
    13021 tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataattaac aacatcagcg
    13081 gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc ctttctggtt
    13141 ccttttttaa ccatatagca agggatcgcc agcgatatta caccagacgg gttcattctc
    13201 gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac cggtggagaa
    13261 agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta acatttacgg
    13321 caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc acgtgctggc
    13381 ggaaatgggc ccgcaaaaac tgcgtgtgct ggatgctggc ggtggtgaag ggcagaccgc
    13441 aatcaaaatg gccgagcgtg ggcatcaggt cattttatgc gatctttctg cgcagatgat
    13501 cgaccgcgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc aatttataca
    13561 ttgcgccgct caggatgttg cttcgcattt ggaaacgccc gttgatctga tattgttcca
    13621 tgcggtgctc gagtgggtgg ccgatccccg cagtgtattg cagaccctgt ggtcagtgtt
    13681 gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt tgatgcataa
    13741 catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga aaaaacggac
    13801 gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg aagaagctgg
    13861 ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc gcgagaagca
    13921 ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt gccgtcagga
    13981 accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc agagcaagga
    14041 taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc cagaaaaaat
    14101 gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt tgccacgctg
    14161 aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc attccgccat
    14221 gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa taacgcgatc
    14281 aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc ggaagggaac
    14341 gcaatttacc gtctgacgcc gctcggcatc ggcattactg actactacat ccgtcagcgc
    14401 gagttttcta cgctgcgtct ttctatgcag ttgtcgattg tggcgggtga gctcaaacgc
    14461 gcagcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa tgtctatgcg
    14521 ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca acgtctgatg
    14581 gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa agactggcgg
    14641 gcggcgattt ccagctgtga attgttgctt tcggaaactt ccgggacgct gcgtgaattg
    14701 caggatacgc tggaagcggc aggcgacaaa ttgcaggcta atctgttgcg cattcaggat
    14761 gcgacgatga cccatgacga tctgcatttc gtcgatcgtc tggtgttcga tctgcagagc
    14821 aaactcgatc gtattatcag ttggggccag caatccatcg acttgtggat tggctacgac
    14881 cgccacgtac acaaatttat tcgtaccgcg atcgatatgg ataaaaaccg cgtctttgct
    14941 cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct aacttatgcc
    15001 aatgccgatc gtctgctgga tatgcgtgac gaagagatgg cactgcgcga tgaagaagtg
    15061 actggggaac ttcctgagga tctggaatac gaagagttta acgagatccg cgaacagctg
    15121 gcggcgatca tcgaagaaca acttgccgtg tacaaaacca gacaagtgcc gctggatctt
    15181 ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt tgacgttgcg
    15241 cgtattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt caccggactg
    15301 ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca tgtcatcgac
    15361 aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc cgttatttcc
    15421 ggcgctggat agcgccttac gttcaggccg ccatattggc ctcgacgaac tggataatca
    15481 tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt ataacgttga
    15541 gcttattcgc gcaccggaag ggttcttcta tttacgccca cgttccacca ctcttatccc
    15601 gcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt atctctatct
    15661 cagcccggaa cggctggcga atgaggggat ttttactcag caggaactgt acgacgaact
    15721 gctcaccctg gccgatgaag caaaactgct gaaactggtg aacaaccgtt caaccggttc
    15781 agacgttgac cgtcagaagt tgcaggagaa agtacgttct tcgctcaacc gtctgcgtcg
    15841 tttaggcatg gtgtggttta tgggccacga cagcagcaag tttcgcatta ccgaatcggt
    15901 gttccgcttc ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac agcgtcgcct
    15961 gattcgtgat ggcgaagcaa tgccgattga aaatcatctg caactcaacg atgaaaccga
    16021 agagaatcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa atttcgctca
    16081 ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga cgagctggtc
    16141 acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc gttcgttacg
    16201 gcgctgatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc cggggccacc
    16261 agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg ttattcgatg
    16321 ctcgacacca ttaactcgcg ccaccagcgc gtggtggtcg gtgtgcgtct gcaacaggtt
    16381 gccggacgcg atcgtaaagt ggatatcaag ccgtttgcca ttcagggact gccgatgtcg
    16441 gtgcagccga cacagctggt gaccgaaacc ctgaacgaac gccaggcgcg cgtgctgccg
    16501 cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa acagttcaac
    16561 tccattactg attaccactc gctgatgttc gatctgggca tcatcgcgcg tcgtctgcgc
    16621 tccgcatctg accgtagcaa attctatcgt ctgatcgaag cttcgctgta tggcgggatc
    16681 tccagtgcca ttacccgttc tctgcgcgac tacctgttgc cggaaaacag cggcgtgcgt
    16741 aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gtatgacgct ggaagcgatt
    16801 cgtgtcaccc agtcggaccg tgacctgttt aagcatctga tcagcgaagc taccaactac
    16861 gtggcggcgg actacatgcg tcacgccaac gagcgccgtg tccatctcga caaagccctg
    16921 gagtttcgtc gcgagttaca tacttcgcgt cagcaactgg cggctgagca gtacaaacac
    16981 gtcgatatgg cgcgtgagct ggcagagcac aacggtgccg aaggcgatct ggaagcggat
    17041 tatcaggcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca gcaggagaag
    17101 atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga gcaaaacgaa
    17161 gtggtggcag aagccatcga acgccaggaa gagaacgagg ctcgcgcgga agctgccgaa
    17221 ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct ggacgtccag
    17281 caaacgcgcg cgatccagta taaccaggcg attgctgcgc ttaatcgcgc caaagaactg
    17341 tgccatctgc cggacttaac cgccgacagc gccgccgaat ggctggaaac cttccaggcg
    17401 aaagagctgg aagcgactga aaaaatgctc tctcttgagc agaaaatgag catggcgcaa
    17461 accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa cggcccgctg
    17521 gcgcgtaacg aggcgtggga tgtcgctcgc gaactattgc gcgaaggggt cgatcagcgt
    17581 cacctggcag agcaggttca accgttgcgg atgcgcttaa gtgaactgga acagcgtctg
    17641 cgcgagcagc aagaagccga acgcctgctg gcagacttct gcaaacgtca gggcaagaat
    17701 tttgatattg atgaactgga agctctgcat caggagctgg aagcgcgcat tgcctctctt
    17761 tccgatagcg tgtctaacgc ccgtgaagag cgcatggcac tgcgccagga acaggaacag
    17821 ctgcagtctc gcattcagag tttgatgcag cgtgcgccgg tttggctggc agcgcaaaac
    17881 agtctcaacc agttgagcga gcagtgcggt gaagagttta cctccagcca ggacgtcacc
    17941 gaatatctgc aacagttgct ggagcgtgag cgagaggcga ttgttgaacg cgatgaagtg
    18001 ggcgcgcgca aaaacgctgt cgatgaagag atcgaacgtt taagccagcc tggcggctct
    18061 gaagatcagc gtctgaacgc gctggcggag cgttttggcg gcgtgctgct gtcagaaatt
    18121 tatgacgacg ttagcctgga agatgcgccg tacttctcag cgctgtatgg cccgtcacgc
    18181 cacgccatcg tggtgccaga tctgtcacag gtaactgaac acctggaagg cttgaccgat
    18241 tgcccggaag atctctattt gatcgaaggg gatccgcagt cattcgatga cagcgtgttc
    18301 agcgttgatg agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca gtggcgttat
    18361 tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg tattgaaagc
    18421 ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt tgatgtacag
    18481 aaaactcagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct ggcggttgcg
    18541 tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt cgaactggag
    18601 cgggcgttaa gtaatcatga aaatgataac cagcagcagc gtattcagtt tgagcaggcg
    18661 aaagagggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt ggctgatgac
    18721 agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca ggaagccgcg
    18781 cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgattgt ttcggtattg
    18841 cagagcgacc cggaacagtt cgaacagtta aaagaagatt acgcgtactc tcagcagatg
    18901 cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg tcgtgcgcac
    18961 tttagctatt ctgactcggc agaaatgctt agcggtaaca gcgatctcaa cgaaaaactg
    19021 cgtgaacgtc tggaacaggc ggaagcggag cgtacccgcg ctcgcgaagc gttgcgcggt
    19081 cacgcagcgc agttgagtca gtacaaccag gtgctggctt cgctgaaaag ttcttacgac
    19141 accaaaaaag agctactcaa cgatctgcaa cgtgaattgc aggatatcgg cgtgcgtgct
    19201 gatagcgggg cagaagagcg ggcgcgtatt cgccgtgacg agctgcatgc gcaactgagc
    19261 aataaccgtt cacgccgcaa tcaactggaa aaagcgctta ccttctgcga agcggagatg
    19321 gacaacctga cccgcaaact gcgcaagctg gagcgggatt actttgagat gcgcgagcag
    19381 gtagtgaccg ccaaagcggg ctggtgtgcg gtgatgcgca tggtgaaaga taacggcgtt
    19441 gagcgccgct tacaccgtcg tgagctggct tatctctccg ctgatgattt gcgctctatg
    19501 tcggataagg cattgggtgc gctgcgtctg gcggtggcgg ataacgaaca tctgcgcgac
    19561 gtgctgcgca tgtcggaaga tccgaaacgt ccggagcgta aaattcagtt cttcgtggcg
    19621 gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga tgatccggtg
    19681 gaagctatcg aacagatgga gatcgaactt agccgtctga ccgaagaatt aacctcccgt
    19741 gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa aaccattcag
    19801 cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc gtttggtcag
    19861 gtgaacagcg tgcgcctcaa cgtgaacgtg cgtgaaacgc acgccatgct gctggatgtg
    19921 ctctctgaac agcacgagca gcatcaggat ctgtttaaca gcaaccgtct gaccttctcg
    19981 gaagcgttgg cgaaattgta tcagcgtctt aatccgcaga ttgatatggg acagcgcacg
    20041 ccgcagacca ttggcgaaga actgctggat taccgcaact atctggaaat ggaagttgag
    20101 gttaaccgtg gttccgatgg ttggttgcgc gcagagtctg gtgcattgtc gaccggtgag
    20161 gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga agatgaatct
    20221 cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga tgaagcagcg
    20281 cgactggatg ctcgttctat cgccacgctg tttgaattgt gtgagcgttt gcaaatgcag
    20341 ctcatcatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta taaactggtg
    20401 cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg atttgcgccg
    20461 caactccctg aaacgcttcc aggaagtgac gaagcgcctt ctcaggcgag ttaaaattaa
    20521 ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttccgcact aaaaaagtgg
    20581 cacattacgg cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg
    20641 cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag
    20701 tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa
    20761 acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt
    20821 tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa
    20881 tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag
    20941 ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca
    21001 cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac
    21061 tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct
    21121 tccagcaaca gctggcagag gtggcgattg ccggtttcca gccgcagttt aataaatggg
    21181 tagagttact gaccgatcct ggtgttaacg ggatggcacg cgacgtagtg ctctctgatg
    21241 cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact cgctggctat
    21301 atagcagtaa accttatgca cttgcaacgc cgccgctctc ggtgattaac caatggcagc
    21361 tggcgctgga taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg cagcatccgc
    21421 aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa ccgtggcccc
    21481 aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta ccggcgttgc
    21541 gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg
    21601 atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa
    21661 ctaagccgat ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg
    21721 cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattggggg
    21781 cagatggtgc tattggcccg gcaacacgtg actggttaaa cgtaacgccc gcccagcgtg
    21841 ctggcgtgtt ggctctcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg
    21901 ggatcatggt taacattccg gcctattcgc tggtctacta tcagaacggc aatcaggtgc
    21961 tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgccc
    22021 ttaacaacgt agtggtaaac ccgccgtgga acgtacctcc aactctggca cgcaaagata
    22081 ttctgccaaa agtgcgcaac gatccgggat atctcgaaag ccatggctat acggtgatgc
    22141 gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct acaatcacgg
    22201 cctcgaattt accgttccgc ttccagcagg ctccaggccc acggaactcg ctggggcgct
    22261 ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc
    22321 tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg
    22381 atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt tctgatgcgc
    22441 tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg aatctctact
    22501 acctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat atttacaatt
    22561 atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt
    22621 aaatgaagta gttcggggaa atcagttgtc gtaataatta gcatgattgg gggcgattct
    22681 ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa
    22741 ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta gacttgatta
    22801 tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc gttgcactcg
    22861 gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc ccgcgcattt
    22921 tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag
    22981 gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca
    23041 aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag
    23101 gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc tattgatacc aacaatgaac
    23161 tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg
    23221 atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg
    23281 caggtggtgt aggatattat ccacgtagta actttgtgca tattgatacc gggccagcac
    23341 ggcactggta gtaattgctt aacgaaacag gggcagtatg aactatcgta ttattccggt
    23401 caccgcattc tcccagaact gttcattaat ctggtgtgaa caaacccgtc tggccgcact
    23461 ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca gcggcctgac
    23521 actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag cggcggaact
    23581 ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca
    23641 aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccaga
    23701 tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca
    23761 ttgccctggg catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat
    23821 ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca
    23881 taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt
    23941 tattccgggt cacggaccat tatccacact tggttatgaa cgcctgcata atcccttcct
    24001 gcaagacgaa atgcccgtct ggtaaggcac ataaaaaagc ccgcttttaa tgcgggcctg
    24061 gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct tatccggcct
    24121 acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa gcgtagcgca
    24181 tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt
    24241 taatgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat ctggtgtcat
    24301 cccggccaca tttacgcgac cagaagcaac cgcatatacg ccaaactctt cgcgcagacg
    24361 cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa
    24421 gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg
    24481 ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta acgcatcgtt
    24541 gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcgcg
    24601 aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg cagcaaccag
    24661 agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac
    24721 aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc
    24781 acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caactgagag
    24841 ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca
    24901 gccatggaac agcactacgt cgccagcctg agcttcgttc aggctgttaa tcagtgcatc
    24961 gaagtcaagg gtgtggtttt ctgcatcata ataagcgtat tcacgaactt ccagacctgc
    25021 agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca ctcgcttaac
    25081 gctggtattt tttgccagga aatcggcagc tacgcgtagt gcgccagtgc cccccggagt
    25141 ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc
    25201 ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc
    25261 attttcgagc agatactgtt cagccttttt cacgctggtc aataccgggg ttttgcccgt
    25321 ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa
    25381 cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac
    25441 gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa
    25501 attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga
    25561 aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct
    25621 attagaactg gtaaacgata cccacagcaa cggtgtcgtc agaacctacg cccagtttgt
    25681 tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt
    25741 agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt
    25801 ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt
    25861 gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat
    25921 tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg
    25981 cgtcgtactt cagaccagta gcccactgtt cagctttttt accgttgcca agaggttgag
    26041 cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt
    26101 attcgtagct gatagaaccg ccaacaccgt cgccgttaga acggcgtgca gtgtcacgct
    26161 cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg
    26221 agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag
    26281 tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt
    26341 agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac
    26401 gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt
    26461 cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac
    26521 gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggaaaaat
    26581 aatgcagacc aacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt
    26641 ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc
    26701 gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat
    26761 aagttctgtc aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct
    26821 gcaggcatct ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag
    26881 atttagtttc aaaaagaaaa aatatgtaac caaaagtaa
//
LOCUS       DAMZII010000064        26554 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZII010000064 DAMZII010000000
VERSION     DAMZII010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.064"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(76..1117)
                     /locus_tag="POA77_002579"
                     /pseudo
     CDS             complement(76..1117)
                     /locus_tag="POA77_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(1245..3206)
                     /gene="topB"
                     /locus_tag="POA77_002580"
     CDS             complement(1245..3206)
                     /gene="topB"
                     /locus_tag="POA77_002580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6365984.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(3211..4254)
                     /gene="selD"
                     /locus_tag="POA77_002581"
     CDS             complement(3211..4254)
                     /gene="selD"
                     /locus_tag="POA77_002581"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDI6365985.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(4371..4922)
                     /locus_tag="POA77_002582"
     CDS             complement(4371..4922)
                     /locus_tag="POA77_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDI6365986.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            5083..6939
                     /gene="sppA"
                     /locus_tag="POA77_002583"
     CDS             5083..6939
                     /gene="sppA"
                     /locus_tag="POA77_002583"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDI6365987.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            7106..8122
                     /gene="ansA"
                     /locus_tag="POA77_002584"
     CDS             7106..8122
                     /gene="ansA"
                     /locus_tag="POA77_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6365988.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            8133..8774
                     /gene="pncA"
                     /locus_tag="POA77_002585"
     CDS             8133..8774
                     /gene="pncA"
                     /locus_tag="POA77_002585"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDI6365989.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8867..10225)
                     /locus_tag="POA77_002586"
     CDS             complement(8867..10225)
                     /locus_tag="POA77_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365990.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(10342..11100)
                     /locus_tag="POA77_002587"
     CDS             complement(10342..11100)
                     /locus_tag="POA77_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6365991.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     IRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(11237..12217)
                     /gene="ydjG"
                     /locus_tag="POA77_002588"
     CDS             complement(11237..12217)
                     /gene="ydjG"
                     /locus_tag="POA77_002588"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDI6365992.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(12227..13174)
                     /locus_tag="POA77_002589"
     CDS             complement(12227..13174)
                     /locus_tag="POA77_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDI6365993.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(13179..14015)
                     /locus_tag="POA77_002590"
     CDS             complement(13179..14015)
                     /locus_tag="POA77_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDI6365994.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDANIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(14036..15079)
                     /locus_tag="POA77_002591"
     CDS             complement(14036..15079)
                     /locus_tag="POA77_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDI6365995.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(15096..16475)
                     /locus_tag="POA77_002592"
     CDS             complement(15096..16475)
                     /locus_tag="POA77_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6365996.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(16502..17578)
                     /locus_tag="POA77_002593"
     CDS             complement(16502..17578)
                     /locus_tag="POA77_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6365997.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(17948..18220)
                     /locus_tag="POA77_002594"
     CDS             complement(17948..18220)
                     /locus_tag="POA77_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDI6365998.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(18262..18675)
                     /gene="msrB"
                     /locus_tag="POA77_002595"
     CDS             complement(18262..18675)
                     /gene="msrB"
                     /locus_tag="POA77_002595"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6365999.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            19017..20012
                     /gene="gapA"
                     /locus_tag="POA77_002596"
     CDS             19017..20012
                     /gene="gapA"
                     /locus_tag="POA77_002596"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6366000.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            20096..20980
                     /locus_tag="POA77_002597"
     CDS             20096..20980
                     /locus_tag="POA77_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDI6366001.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(21031..21885)
                     /gene="yeaE"
                     /locus_tag="POA77_002598"
     CDS             complement(21031..21885)
                     /gene="yeaE"
                     /locus_tag="POA77_002598"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDI6366002.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(21975..22721)
                     /gene="mipA"
                     /locus_tag="POA77_002599"
     CDS             complement(21975..22721)
                     /gene="mipA"
                     /locus_tag="POA77_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDI6366003.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            23157..25091
                     /gene="yeaG"
                     /locus_tag="POA77_002600"
     CDS             23157..25091
                     /gene="yeaG"
                     /locus_tag="POA77_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDI6366004.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            25204..26487
                     /locus_tag="POA77_002601"
     CDS             25204..26487
                     /locus_tag="POA77_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDI6366005.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         6854 a         6827 c         6154 g         6719 t
ORIGIN      
        1 atgggcacaa ttcattgcag ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg
       61 caaaatttca ggcgtttatg agtatttaac ggatgatgct ccccagggaa catttcttat
      121 gggccaacgg catttcttac tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc
      181 ttcaagttca gcatccgtta actttctgcg atagcagcag atatgccagt aaagaaatcc
      241 cattagacta tttttttgat aatcttcttc gctttcgaac aactcgtgcg cctttcgaga
      301 agcaagcatt atataatgcc aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt
      361 gcttgatatc gagatcatcc atgataattc cgccgcccat attagcttcg ccgaggattt
      421 accggagcga ttattagcgc aatcagagat atagtctgag ggaaaaacag caaatttatt
      481 caacaaggcg ataacctgct ctggggcttc ctccatgttt gctttaaagg tattggctcc
      541 atggtcgcca gaaagaaaat gctccattaa ggcacaataa ctttcgctat cttcgatacc
      601 ccattgatcc tctaaagact cgcgtctttt acttatgata tcgatcgagt caaagggaag
      661 cacatgatat tggaaggtat ctttgccagg ttcaggcttt cgcggccaga actccagcgt
      721 ttcagaccat tgcttatgat agaaccgata aggtgcgatc aattgtagcg cctgtaactt
      781 ctcgatactg agcggctcaa tacctttagc ctgataataa tgcagttgtt ctttttttgc
      841 tttaaaaccg gcccgaacaa taagccccat cataattaat agataaagaa aagagcatcc
      901 cgcagtaatc aggcctcttt cattcaaacc gttggatgtt atcgctgcga atacaaacat
      961 tacagcgaca acacatgtta aatacccccc cccacttaca aagcagcatg gccttatttt
     1021 ctttaatcat ccgttcaaaa ttactattaa atatttccca gccattaaaa gaatacttct
     1081 cgctcccagg atggttttgt aataaaactt ttttcatcac attccctgtt attacatact
     1141 ttagaggcga taattttatc catgcaaaaa aaatatccaa cgaattttct tgatccgctg
     1201 gatatttcag aatatgactc gaatagcacg aaagattcgc tcgcttacgc tatcgccccg
     1261 cttccggctt catctgctgg cggacttttt ttcgcactac gtttacgcgg tgcagccttt
     1321 ttcttatcag cactgccacc actgcccgga gccacaatgc cgcgaaactg ccgcaccggc
     1381 gtacgtttgg cttgatcaat aagctgatat agcgtcccca ccagcggctg cataaagtcc
     1441 tgatagcgac actgcttttc gctgatttgc gtcagcaccg attcccagtg cgcggtcatg
     1501 tccggtcgcg tagccatttc tggcagcgaa tggaataacg cttttccggc gtcggtggag
     1561 tggatatagc gccctttttt ggtgaggaaa ccacgcttga acaacagttc aataatcccg
     1621 gcacgcgttg cttctgtccc cagaccatcg gtcgcacgaa ggatcttttt cagatctttg
     1681 tcctgcacaa agcgcgcaat cccggtcatc gccgaaagca gtgttgcatc ggtaaaatgg
     1741 cgcggcggct gggtttgccg ctctaccact tcaccttttt cacacagcaa ctcatcgcct
     1801 ttcgccacca caggcaatgg cgtgccgtcg ttttcttcat cgcgctcttt gctgcctaac
     1861 agcgcgcgcc aacctgcttc agcaagaaaa cgcgctttag cgacaaattt gcctttggca
     1921 atgtccagtt cgataacaca cttgcggaac actgcatccg ggcagaactg catcagatac
     1981 tgacgggcaa tcaggttata gaccttcgct tcgttctccg tcaggttgat cgcagaactc
     2041 cttgcggtcg gaatgatggc gtggtgcgca tcgacctttt tgtcatccca acagcggttg
     2101 cgtatatctg gatctaccac tggctgcggc aacagatctg gtgcatgaac gctgatggca
     2161 ttcatcaccg cgtggcgtcc ggcaaaatgt tcttctggca aatagcgaca atcggaacgc
     2221 ggataagtga ttaacttgtg tgtttcgtac agcttctggc agatatcaag cacgttctgc
     2281 gcgctcagac caaagcgttt tgccgcttca atctgcaacg ccgaaagcga aaacggcagc
     2341 ggcgcggatt ctgattcccg tttatcgtta tagctggtga caatagccgg ttgaccacta
     2401 atgcggttaa ccacatgctc cgccagtgga cgatgtaaca agcgcccttc ttcatcctgg
     2461 tacggttcac acgcttcgct cggttgccag atagcggtaa accgctcatc ggcaggtgtc
     2521 acgatatgcg ctttgacttc aaagaagtct ttcgccacga agttttcaat ctcttcatcg
     2581 cggcgcacca ccagcccgag cacgggtgtc tgcacgcgtc ccacggaaag tacgccctga
     2641 taaccggcat tgcgaccgag aatggtatac gcacgggtca tattgatgcc gtacagccag
     2701 tcggcacgcg ctcgcgccag cgcagaaacg cacaatggca caaactcact gttggaacga
     2761 agacggtcga tcgcccgctc aaccgcctgc gggttcaggt cgtttatcaa gcaacgctgc
     2821 acctgctggc gcttttccgg tgccagttgc agatagtcca gcacttcatc caccagcaat
     2881 tgcccttcac gatccgggtc cccggcgtga acgatttcgc tggcttcatg caggaagcgt
     2941 ttgatgacgt taagttgttt ggtcacggag ggtcggggct gtaattgcca cttttccggg
     3001 acaatcggca aatccgcaag attccagcgc gcatagcggc tgtcgtaggc gtctggctgc
     3061 gcctgctcaa gcaggtgacc gatacaccag gtcaccacct gaccattacc gcactcgata
     3121 aagccatcgc ctttacggtg cggtttgggc aggacatcag caatggcgcg cgccagactc
     3181 ggtttttcgg caataaacaa ccgcattgaa ttaacggatc tcaaccatgg cacgaccgcc
     3241 gcgcgctggc accagttcgc caatcgccgt cagttcaatg ccaaactcgg cggctgtagt
     3301 tttaacctca ttttctgctt ccggcatgac cgccagcagc aaaccgccag aagtttgcgg
     3361 atcgcacagc agatcgcgca cttcacgcgg catttcaccc atcagatgac cgtagctggc
     3421 aaagttacgt tcggtgccgc caggcactgc gcccaactta atgtactctt caacccccgg
     3481 caattttggg atcgcgtcat agtcaacgcg cgcctgcaca cctgcgccct gacacatttc
     3541 gctcaaatgg cccagcagac caaaaccagt aacgtcggtc atcgctttta cgccttcgat
     3601 gttggcaaag gacgcacctg cgatgttcat ccggcacatc acttccgtcg ccagtccctg
     3661 atgttctggt ttcaacagtg attttttctc agccgtggta agaacgccga tccccagcgg
     3721 tttcgtcagg aacagtttgc atccggcttg tgcggtactg tttttcttca cccgctcggt
     3781 cggtacgatc cccgttaccg ccagaccaaa aatcggctcc ggcgcatcga tggagtgacc
     3841 gccagccagc gcaatacccg cctgacggca tgcatagcgt ccaccttcgg ttacttcgcg
     3901 ggcaatttct ggggaaagtt tgttaatcgg ccagccgagg atcgcaatcg ccataatcgg
     3961 tttgcccccc atcgcgaaga tatcgctgat ggcgttagtc gccgcaatgc ggccaaaatc
     4021 gaaaggatta tcaacgatcg gcataaagaa gtcggtggta ctgataacgc tggtgccatt
     4081 gcccagatcg tacaccgccg catcgtcgcg ggtctcatta cccacaagca aattcggatc
     4141 aacaaacttc gcctgctcac tgtgcaggat ggtttccaac acttttgggg aaattttaca
     4201 gccgcaacca gctccgtggc tgtattgggt caaacgaatc gagttctcgc tcatggacat
     4261 ctcctgtcaa tgcaatccgg gtatggtaac cctcattccg tgaagtgata agtgagagtg
     4321 tctgaattcc tgcgcctttg ctcacaatcc agacagtttc gcgacaatta tcagaaataa
     4381 gttacaaacg gcgtcgggtc cgggacgtta atcgacgtag atgctttcaa ctgcggcgtg
     4441 ccgaggtaga gaaaaccgac aattttatcc tgctcacggc aaccgaatgc ttcacgcact
     4501 accggacttt cagttaatgc gccactgcgc cagatgccgc caaatccttg ggcaacggct
     4561 gccatttgca tcgccatgac cgcgcatccg gcagacattt cctgttccca tcgcgggact
     4621 ttatgatttt cttcgcattt cgccaccacc gtgatgatga gcggtgcgcg gaacggcgca
     4681 ttacgggctt tgtcgatagc tttgtcatca ctaccggcag caatcgcccc ctgttccagt
     4741 acggcgctga aacgctcgcg cccttcccct tcaatcacaa aaaaatgcca cggttgcatg
     4801 gacttatggt ccggcgcacg catacccgca cgcaggatgt tttgcagttg ttcacccgtt
     4861 ggcgcgggtt cagccaggcg ggaggcgcta cggcgattga tcaatagttc gagtgcatcc
     4921 atttgattaa ctcctgtcgt gatatttatt cacaaaatta acacgagagt ggattttgtt
     4981 acagcacagt ccgcaattcc tgctgacaag tgccggttgg gtcattacga taaccacatc
     5041 tattgcgcct gtgacaggtg tgaccttaag ttgggagaat acatgcgaac cctttggcga
     5101 tttattgccg gattttttaa atggacgtgg cgtctgctga atttcgtccg tgaaatggta
     5161 cttaacctgt tctttatttt cctcgtactg gttggtgtgg ggatctggat gcaggtcagt
     5221 ggtggtgatt cgaaagaaac ggccagtcgt ggcgcactgc tgctggacat ttctggtgtg
     5281 atcgtcgata aacccgacag ttctcagcgg tttagtaaat taagccgcca gctgcttggt
     5341 gccagttccg atcgtctgca ggaaaactca ctgtttgata tcgtcaacac cattcgccag
     5401 gcgaaggacg accgcaatat caccggtatc gtgatggatc tgaaaaactt cgcaggcggc
     5461 gaccagccgt ctatgcagta catcggcaaa gccctgaaag agtttcgtga cagcgggaaa
     5521 ccggtttatg ccgttggcga gaactacagc caggggcaat attatctcgc cagtttcgcc
     5581 aataaaattt ggctgtcacc gcaaggcgtg gttgatctgc acggttttgc taccaacggt
     5641 ctgtactaca aatcgttgct ggataagctg aaagtttcca cccatgtgtt ccgcgtgggt
     5701 acgtataaat ctgccgttga accgtttatt cgtgatgata tgtcaccggc agcgcgcgaa
     5761 gctgacagcc gctggattgg tgagctgtgg caaaactatc tgaatactgt tgccgctaac
     5821 cggcagatcc ctgctcagca ggtattccct ggcgcgcaag ggttgcttga gggtttaacc
     5881 aaaaccggtg gcgataccgc gaaatatgca ctggaaaaca agctggtcga tgcactggca
     5941 tcgagtgccg aaatcgaaaa agctctgacc aaagagttcg gctggagtaa gactgataaa
     6001 aattatcgcg ccatcagtta ttacgattac gcattgaaaa cgccggcaga taccggtgac
     6061 agcatcggtg tcgtcttcgc taatggcgca attatggatg gcgaggaaac tcaggggaat
     6121 gttggcggtg ataccactgc ggcacaaatc cgcgacgctc gccttgaccc gaaagtgaaa
     6181 gcgattgtcc tgcgtgttaa tagcccaggc ggcagcgtta ccgcgtctga agtgattcgc
     6241 gctgaactgg cagcagcccg ggcagcgggt aagcctgtgg ttgtatcgat gggcggcatg
     6301 gcggcatctg gtggttactg gatttccacg ccagctaatt acattgtggc taaccccagc
     6361 accctgaccg gttctatcgg tatcttcggc gtgatcacca ccgtagaaaa tagtctggat
     6421 tcgattggtg ttcatactga tggtgtctca acttcaccgc tggcggatgt ttctatcacc
     6481 agggcactgc cgccggaagc gcagcagatg atgcaattaa gcattgagaa tggctataaa
     6541 cgctttatca cgctggttgc tgatgcgcgt cattcgacgc cggagcaaat tgataaaatc
     6601 gcccagggcc acgtctggac cggtcaggat gcaaaagcta acgggctggt cgatagtctc
     6661 ggggatttcg atgatgcggt tgccaaagca gcagagctgg caaaagtgaa acagtggcat
     6721 ctggaatact acgttgatga accgaccttc ttcgacaaag tgatggacaa catgtctggt
     6781 tctgtccggg caatgttgcc agatgcgttc caggccatgt tacctgcacc gcttgcctcg
     6841 gtagcctcta ccgttaaaag tgaaagcgac aagctggccg cgtttaacga cccacaaaac
     6901 cgttatgcgt tttgcctgac ctgcgccaac gtgcgttaag tcttgtactg agtggccgac
     6961 agatcgtcgg ccacattatt ttttacctcg acgaatcctc ttcccgctgt ctcgccccat
     7021 atttccttat ccgcatagta tcaggtgcgc tcctcctgcc tcacgtatat acttttgctc
     7081 tttcgatatc attcatatca atatcatgca aaagaaatca atttacgtcg cctacacggg
     7141 cgggaccatc gggatgcagc gttccgagca gggttatatt ccggtgtcag gtcatctaca
     7201 acgccaactg gcgctgatgc cggaattcca tcgcccggag atgccagatt tcaccattca
     7261 tgaatatacg ccgttgatgg attcttctga tatgacgccg gaagactggc agcatattgc
     7321 tgaagatatt aaagcgcact atgacgacta tgatggtttt gtcattctac acggtaccga
     7381 cacgatggcg tataccgcct ctgcgctgtc gttcatgctc gagaatctcg gtaaaccggt
     7441 cattgtgaca gggtcacaaa tcccgctggc tgagttacgc tctgacggac aaattaatct
     7501 gctgaatgcg ttgtacgttg cggcgaatta tccgatcaac gaagtaacgc tctttttcaa
     7561 taaccgattg tatcgcggca accgcactac caaagcccat gccgatggtt ttgatgcgtt
     7621 tgcctctcca aaccttcctc cgttactgga agctggtatc catattcgtc gtttgaatac
     7681 gccacccgcc ccgcacggtg aaggggaatt aatcgttcat ccaatcacgc cacaaccaat
     7741 tggcgtagtg acgatttatc cagggatttc tgctgacgtc gtgcgcaatt ttctgcgcca
     7801 accggtgaaa gcattgattc tgcgctccta tggcgtgggt aatgcgccac aaaacaaagc
     7861 cttcctgcag gaattacaag aagccagcga tcgcggtatt gtggtggtca acctgacaca
     7921 atgtatgtcc ggtaaagtga atatgggtgg ttatgccacc ggtaacgccc tcgcccatgc
     7981 cggcgttatt ggcggtgcag atatgactgt agaagccacg ctaaccaaac tgcattacct
     8041 gctgagccag gaactggata ctgaaaccat tcgcaaggcc atgagccaaa acctgcgcgg
     8101 cgaactgacg ccggatgatt aaggagactg caatgcccca tcgcgccctg ttactggtcg
     8161 atttacaaaa tgatttctgt gctggtggcg cgctcgccgt gccggaaggt gacagtacgg
     8221 tagatgtcgc taaccgcctt attgactggt gccagtcgcg cggagaagcg gttatcgcca
     8281 gccaggactg gcatccggcg aatcacggca gttttgccag tcagcacggt gtagagcctt
     8341 atacgccagg ccaactcgac ggtttgccac aaaccttctg gccagatcac tgtgtgcaga
     8401 acagtgaagg cgcacaatta catccgttac tgaaccaaaa agcgatcgca gcggtgttcc
     8461 ataaaggcga aaatccttta gttgacagtt acagtgcctt ttttgataac ggccgtcggc
     8521 agaaaacctc tctcgatgac tggttacgcg atcatgaaat cgatgaattg atcgttatgg
     8581 gcctggctac tgactattgc gtgaagttta ccgtgctgga cgcgttacag ttaggttata
     8641 aggtgaacgt gattaccgat ggttgtcgtg gcgtgaatat ccagccccag gacagtgcgc
     8701 acgcgtttat ggagatgtca gcagctgggg caacgctata tacgctggca gactgggaag
     8761 agacgcaggg gtaattttac gctggcctac aattctgtac tggcattgta ggccaaataa
     8821 aacacgtcag tggcacatct ggcaattgat gccatcaacg aaagatttaa ttcacctcag
     8881 aaatctcttc caacgacacc ttccgcgttt cgatgccaaa aatggagaga accagcgcac
     8941 aaagcaataa catgacacca agtaccataa acaccgtgat cgacccataa tgtgttaata
     9001 atgccgcaac gccatagggc gtgaaaactg cgacgatccg tccgacggca ttaacgaaac
     9061 ccgaaccgcg caggcgtaaa tgcgttggcc aaagctccgg gatataaacc gccgacgcga
     9121 agcaaacata catgtataaa aagaagatca tcaccaggcc atagattaaa atcgcccact
     9181 ctgtagtctg aattgaatag atatagccta acacggcaat aataataagt aaggtggagc
     9241 caaataaccg acgaggaaaa tgatcaataa ttaatgccgc aataaatatt cctaccggag
     9301 cgccaatcat aataacagcg gtcattaata ttgatttatc gacatcaatg ccggagttaa
     9361 caaatatggt cggtatccat acggtgatgg tataaagcga gatgttcatt gcaattaaaa
     9421 cagtaatcgc gactaatgta cgtcgtaaca tttcaccttt aaacaggagc cagaaagtac
     9481 ccttgattac tttaaccttg ctgttgctct gatacgaagt aagcgggggt aaacgaatac
     9541 tcttctctct ttcaatttgc tgctctactt cacgaagttg gctttctgca ccggcgattt
     9601 gccctttccc tgccagccat cgtggcgact cgataaagta tttacctgag agaaaccagg
     9661 ctaacagtat gccaatacca cccagcagaa acattattcg ccaactaaaa aaagcgataa
     9721 ccaccacgcc tatcgccgca gacagcatgg gcgaccagtt accaacaaat gagagccgcg
     9781 cggaccattt tccacgcacc gtcgcgggga taaactccgt aaatgaggca tagccaacca
     9841 taatcagcgc ccccattcct gttcccatca ggaagcgaaa gaagatgagc cagtacatat
     9901 cagggacaaa agcggcccct gttgcagcaa taccgacgat gagaagattt atgcgaaacg
     9961 ccctgcgccg cccaaagtag tcaccaataa acccaccagt aagtgagccg atgaaataac
    10021 caaacattaa tgccgaggta aaggcggcat tgaggaaatt atttgaccat ccattgctta
    10081 ccagctttgc taagacgaca ttaccggagt aactcaaaaa ccctgttaat aacagactaa
    10141 agcttataat accaaatata cgataatgaa accgggccaa aggcaagcgg tccagtcttg
    10201 cgccaatttg atcatattgt tccattaggt gcctcggatt tgctaaaaag gtagcaattg
    10261 cggcaaattc acttgccgca tctttatcat cattttattt caaggcgcag gaacaaataa
    10321 tattgacaac gtgtgcttaa attaccacac cagctggata ttattgtctt tgcaaaatgc
    10381 tatccactct gcacccggtg atttatcagt tattatgtaa ttaatatgac taaaatcagc
    10441 taactggaca aaagctttgc gatcaaactt agagtgatca accaataacg caacttctgt
    10501 cgcctgacgg atcattgtct ttttgatttc agcttccgct tcattagagt ccagcgcgcc
    10561 gctgttaata tcaagacctt tacaactcat taccatgata tcgacatgat agcgcctgat
    10621 gatctcttta gtaattcttc cttgcagtga aagtgtattt ttgtttagtt ccccacccgt
    10681 tgaaacgact ttaatttctg actgagccag aacatgaatc gcttccgccg agttggttag
    10741 caacgtcagg ccactacggt cctgtaacaa tttgagcaat tccattacgg tactactgga
    10801 atcggctgcc atggtggttt tattgtcgat aaagggtagt gccttgcgtg caataagctg
    10861 cttctcttca taaaacgatg aagcgcgctt ataaaaatgg atattctccg tcaacatcgc
    10921 tgtatttaaa acagcaccac cataggttct ggtcaaaaag ccttcatctt ccagcttctc
    10981 aagatcgcgg cgaatggttt cttcggttac ctgaaaaatc ccactcaaat ttgagactgt
    11041 caccttttta tcgttggcaa ccatttgctt aattgcctga atcctgtctt ttgccgccac
    11101 gattacaccc ctgtatcttt ttacatcaca ttagcgcgat tatcgcataa ccgatgttta
    11161 ctttcaaaat aacctgtttg aatcacagat tttcatcaca gttttcacag aaacagaggt
    11221 gaatcgtgtt gagtatttaa cgctccaggg cctctgccat ttccctcatc aatgttgcgt
    11281 ctgcatccga taagttgata ttcagtgccg cgacattttc gcgtacctgt tccggtgcag
    11341 tagccccact aagaatggag attaaatcac tctgttttaa tatccacgcc agtgccagag
    11401 tgggaattgt gcactgataa cgagcacaaa gtggctgcca ctgttcaagc atatcaatca
    11461 ctttcagcat gttttcacgc tggaaccaga ctttatttgc ccgagcgccg cccggaacgt
    11521 aatcacgagt gatggtgccg gtcaacaatc cctgctctag cggggaataa acctgaacca
    11581 caatgccatt atcacgacat agtggcagca gttcgttttc cattgcccgg tcgaggatac
    11641 tgtatttcgc ctgaataata tccagttcac catattgcag atactcgcgg atatggtcag
    11701 catcgacgtt agcagcgcct atagcgcgaa ttttcccttc agactttaac tcattaagca
    11761 ctgcgacagt ttcagcgatc ggcgtaaaaa atggcggcac cgactgccag tgcgtcatgt
    11821 agatatcgat gtaatcaata cccagacgtt gcaagctcgc tgctacctct tcgcggatag
    11881 attccgggga aaggttttta tacaactgcc gatcgccaac tttgttgaat aaacttcctt
    11941 ttcgttccca gacaatgccg catttggttt ctactacaac ctgttcacgg ggcagttttt
    12001 ttaacgcctg accgacgata acttcactat tgccaaagtt atatcctggc gcagtatcaa
    12061 tcagattaat gccacaacga tgggcttcaa gaatcgtatc aatacatatt tgccgatcga
    12121 gatcgccatt ccatgcagga ccgccgccaa tggcccatgt ccccaacccc attcgcgaaa
    12181 gcgtaatatc cgttgtgcct aaaggtatct ttttcatttg ccttcattat ccttcgtatt
    12241 cttccagcaa ttgttcgacc agctttctgt tttttacgcc ggtggtggca ccgacgctta
    12301 gaaccgagat agccgccgtt gcattggcaa agcgtgcgca ttcacgcaga tttttgcctt
    12361 ctaacagtgc cgcaataaaa cctgaagcaa agttatcgcc cgcgccaatg gtgtcgatgg
    12421 cggttattcc tgcgaccgcc ggcaccttca tcgtcatgtc accacgcttg ataaagcagc
    12481 cgtctttacc cgttttaatc accaccgttt ttacgccgca cgcaagaaag cagtcagcaa
    12541 tttcatccag tgtctctttc ccggtgagta attttgcctc ggcaaaatta ggaaacagat
    12601 aatcgacata actcaatgct tcgcaaatat catccagcgt ttcattcaac cgcggtttga
    12661 tcatatcggc acagataatc atctgccggg ctttagcttg cgtaaaaatt tctgttagcg
    12721 ctttaccatc caatagtgga ctgttgaaaa tactggccag tgataataat ttcgcctgag
    12781 aaaaccgcgc aaaatcaaca tcgtcaatat tcaatttcca cagactgcca ttacggtttg
    12841 tgacaaacgt ccgctcgcca tcctccgtca ccagtccaac gttaatagag gtatctatgc
    12901 tgacgtcctg cttcaggctt tgaatatcaa tattctcttt gcggcaatgg tcgagaataa
    12961 attgccctgc ggcatcttta ccaatacgac tcattaatgc tgtgcgatgg cccagacgag
    13021 aaataattgt tgcttcattt attgcatcac ccccggtggt cattgcgatt ctttcaagag
    13081 gataagaatc cacatcaaag atatttttac tgaccggttg caatggaata tcaacaatag
    13141 cggcacctat acaaataacg tcgagattat ccatatcatt attccgcttt gccatcagaa
    13201 ccgaacagtt taattttttc caatgcccgt tcttttacag ctttacgtac ttcacgttct
    13261 aaatgcagga aaggctgatc ctggttttct ttcactgcaa ccattgcggc ctgacagagc
    13321 tccgtatgaa tgttgatttt cgcgataccc aacgaaattg cagttttaat gttggcatca
    13381 ctgatccccg atgcaccgtg caaaaccagc ggaacagaaa cggcatcgcg tacgcgtttg
    13441 acgacctcaa agttcaattg tggctctgac gtataaaccc catgctggtt gccgatggcg
    13501 acagccaatg aatcacagcc cgtacgttca acaaattcag ccgcctggtc aggatcggta
    13561 tagtgatagc ccgccagcgc ttcctcataa acggtttcat taccgacatg ccctaattcc
    13621 gcctctaccg gaatacccag cggatggaag aaatcgacag cctctttggt taaacgaata
    13681 ttttcttcga aatcaaacgc ggaagcatca cgcattaatg aattcatacc atgagtccag
    13741 gcgttatgaa taatctccat actccgacca tgatcccaat gagttattac cggcaccgtt
    13801 gctttttgtg ccattgatac catcatgtga gagaaatctt caaatgaggt gttaccgaca
    13861 aaacctgtac caaaagaaat aataaccggg gatttcgctt cttcggctgc gtcgataacg
    13921 cccatcaaca tttctgcatt ccatacgtta aaatgggcaa ttgcataatg tttatttgtg
    13981 gcatcgtttt cccaatatct gatatctgcg agcatgttct gttctcccgt attaattaat
    14041 cgctaatttt aataacgcct ttaataatgt cgcgtttgtt gttaactgac tcttcaaatg
    14101 cctgttgtac atcccgataa tcgtaaatat gcgtcaccat cgatttcaca tcgaatcgcc
    14161 ctgaagaaat agcttcaatc gtgaccggat aacgattggc atagcggaat accgtctgga
    14221 tagtgacttc gcgattgatt ttgaggaaat tgattgccga atcgccgggt acagtaccaa
    14281 caatcataat tttaccgccg cgcattacca gataaggtgc ctgtttaacg gtgaccgcag
    14341 aacccgctgt ttcgaaaaca atatctgcgc ccatgtcttc ggtaaattgc tgacagcgtg
    14401 caatagtgtc ctcttttgcg ccgttaataa ccactgtcgc accaagctgt tccgccattg
    14461 ccagacgttt ttccagtaca tcaacgacgg caatttccgt tgcgcccagg catttgcacg
    14521 cttgcaacgt catcaaacca atacaacctg ctcccagaat aattatcttc ttacccggtt
    14581 taacatctgc cagcatcgcg gcatgcatcc cgactgcggc aggctccacc agcgcccctt
    14641 ccatcgtgtc catattgtcg ggcagtttgt aagtaaagct ctccggatga cacagatagt
    14701 gcgttaatgc gccgcggtag ttgggttgtg tcgccataaa atcaacgtcc gggcagatgt
    14761 tatatttgcc ttccagacag taacgacagt gaccgcaagg aacgccaggt tcgatattta
    14821 cccgatcccc tggtttaaat ttgcgcacgc ggcttcccac agccacaacc gtcccggcgc
    14881 attcatgacc caggccaatt tcttgatttg ggtctttagg aggaataaac gggcctgatt
    14941 caaaaccatg tacatctgaa ccacaaatac cgacatattc tactttaatc aaaacttcat
    15001 cttctttagg cacaggtatt tctgctgaaa taattttcat tgtgcccggc acctgcaata
    15061 ttgcttttga atttttcatt ttgaatccac cagtattatt tattggctac tgcatcaata
    15121 cttaaacttt caacggagac acctttggtt tcaattccaa tggtggcgat agcaattgcg
    15181 acaataattg aaacagcccc cagaagaata aagactcccg ttacgccata actactgagc
    15241 agcactgcaa cggcataagg tgcggcaata ccactgattc gccctaccgc attcgccaga
    15301 ccggagccac ggagtttggc ctctgtcggc cagatttcag ggacatacac tgccgaggca
    15361 tagcaaacgt acatatagac gaaagtaatc aggaagaaac caatcaacgt tatgagcaac
    15421 atactggttt gcagtgaata gatatatccg agcaccgcaa tcaggattaa tagccccaca
    15481 cccattgttt tacgcggaat tttatccatc accagcatgg caataaaaat accaaatggc
    15541 gcgccaaaca tactcatggt atttaaaaca atcgagtctt ttaaattaat cccctgggtc
    15601 atgaatattg ttggcaacca gttaattagt gtgtactgca caacgttcat ggcaatcagc
    15661 acacaagagc ctaatatcac gcgtttcagt aatactcctg tcagtaaggc tgaatacggc
    15721 accgcttgtg gcgctttacc gtcatcagca ataaccacag gcggcaaagg tttacccgtc
    15781 tggcgtatga cgccttcttc tatactccgc atgactttct cggcttcctg ataccgtccg
    15841 cgcgattcca gccagcgcgg ggattcagga aagtagcgcc aggccagcgc cgtagcgata
    15901 agcgacaata ttgcagggat aagcagttgt acccgccagt tccactctgc actaatcagc
    15961 ggcgtgagtc ccatcgctat caatgaacag agcggatatg accagttgcc aataaaggaa
    16021 acccgacttg accacgttcc acgatttcta ccgggcatat attcggtgaa accagcaaac
    16081 agtgtaacca gtaaagctcc cagcccaacg cccatcacaa aacggcaggc aataagaaaa
    16141 tccatattcg gtgagaaagc accgacaacc atcgaggcaa tatgaatggc ctcatagagg
    16201 atgaacgcat ttctgcgacc ggttttgtcg ccaatgatgc cgcccaccag cgcaccgaga
    16261 aacattccgg cggtcgtgat tgctgagaat gtggctgtgg tggaattatc tgtccagccc
    16321 aacgctttca gctgcgcgag gatcaagcca ccaacggcat tactccagca gacaagcaag
    16381 ccaaacgcga cgatggcaaa cattgacgaa tgccagcggc aatccggtaa gcgatccagc
    16441 cgtgcaccac aatgcggttt tgttatctgt tccatgcgat aacgttcctt caggagagtg
    16501 gttattcatc aaagtcgtaa gtcatgatca ctttgattgc ggttttatcg accatcgcat
    16561 caaacccttc gcgccattgc gacaggccga tacgatgcgt gatcatcggt ttgactttga
    16621 tagcgccgct ggctaatagc ctgatagcgt tacgccatga ggtggagtca taggccatat
    16681 gcccaatgat gcttttgttc caggcggtaa tgtcattaat cgagaaatca agaggtttga
    16741 agcccattcc aacgcgtacc acttccccgt tcgggcggag catttcgatg gcttgtttca
    16801 gtgcgatatt ggcaccggag cattcaatca ccagtcccag attatctttg ccacaaattt
    16861 gctggcagcg cgccaccaca tcttcggtag aaccatttac tactgccgtc gcacccagtt
    16921 cttttgcaac cgggaagcgg accgccacat cttcttgcag accaacgacg acgatattta
    16981 ccgcccccat aattcgcgcc atttgtacgg agaacagccc gagtgggcca gtgccgatga
    17041 cgaccacatc ctgaccagga aggaatttcg attgctgcgc gatggatttg taggcattac
    17101 agatagggtc aagtacggct gcgtcctcat aatcaacacc atcagggatt tcccacaacg
    17161 catgacgatg aattttgaga atttcaccag gaaccagaca atatttggaa aaaccaccgc
    17221 cccaggtatt attatccaga ccaaggttta ctttttctgt acaacacaga aaatcacctt
    17281 gttcacaggc cggacaaacg ccgcaaacgt gaccgctgtt atccgacaca acgcgttgcc
    17341 ccactttcca gtctttgact ttttcaccaa cctgcgcaat acaacctgcg aactcatggc
    17401 cgcggataga gttaaactca tcagaaccgc tatcgacatt gtagtgcttc atgtctgcgc
    17461 cgcagattgc cgcggcttta atttcaatca ctacgtcttc cgggccacaa atgggttgtg
    17521 ggacatcaat catcttgtag ccgccaaagg ccttgccaaa ccgagccagt gctttcattg
    17581 cgtcatcctc tgtgttttgt tttgcttaga tggccatcat acttacccac atgatttttg
    17641 aggtcaacat taaatctgca aaacacagat aatgatctgc gttttacaac ttagatcaca
    17701 attacgcaga aaatggctgg gtctgttaca ggttggtgga aggcggaacg caaaaagagc
    17761 aaattcaaga gggattaccg gccaaatagg tctgagactt aagtccagga gaagtcattc
    17821 aggcgtgaac gcgacggcaa gagatcggac tgggacttag ccaggtcggg tcgagataat
    17881 tgagacgagt cgtggggtca ttgttggcta tccccttcat agccgcggca aaaaggggat
    17941 gaaccactta cttaaacatg gcaataggct ttgctgagat accaaaatct tcttttaact
    18001 gctgtttgct cttcatgacc atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg
    18061 tattatggcg ggcttgccac agcatcacca gttgcaggca attctctttt tgttcctcgg
    18121 tcaacgcaac gccatcaggc catttcccca gttcaacggc ggtcgacaaa cgctggtata
    18181 cctcaggcgt catgctgttg ataatgtcat caagattcat aatttttccg ctcctgtgga
    18241 ataagttgct gaatcgtttt ttcaaccgtt gatttcttcg ccgttttcgc catcggtaaa
    18301 gcgtaaagag gcagagttaa cacaataacg ttcgcctgtt ggttgcggcc cgtcgggaaa
    18361 gacatgcccc agatgggcat cacagttacc gcaacgaatt tctatgcgct gcattccatg
    18421 tgacaagtct ttgatataac gaatggattc ttcactcacc ggttcgtaga aactgggcca
    18481 gccacagccg gaatcatact tggtttcgga atgaaacagc ggggcatcgc agatcaaaca
    18541 gtgatatacg ccgtcacgct tgttatgcag taaacgaccc gtaaatggcg gctctgtccc
    18601 atgattctgc gttacgtaaa actgcatctc ggacaaattt tttttcagtt cttctgccga
    18661 aggtttatta gccatttgct cacatctcac tttaatcgtg ctcacattac gtgactgatt
    18721 ctaacaaaac attaacacca actggcaaaa ttttgtccta aacttgatct cgacgaaatg
    18781 gctgcaccta aatcgtgatg aaaatcacat ttttatcgta attgcccttt aaaattcggg
    18841 gcgccgaccc catgtggtct caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg
    18901 cttaggcaca ggattgattt gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag
    18961 gtttttgtaa ttttacaggc aaccttttat tcactaacaa atagctggtg gaatatatga
    19021 ctatcaaagt aggtatcaac ggttttggcc gtatcggtcg cattgttttc cgtgctgctc
    19081 agaaacgttc tgacatcgag atcgttgcaa tcaatgacct gttagacgct gattacatgg
    19141 catacatgct gaaatatgac tccactcacg gccgtttcga cggtaccgtt gaagtgaaag
    19201 acggtcatct gatcgttaac ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta
    19261 acctgaaatg ggacgaagtt ggtgttgacg ttgtcgctga agcaactggt ctgttcctga
    19321 ctgacgaaac tgctcgtaaa cacatcaccg ctggtgcgaa gaaagtggtt atgactggtc
    19381 cgtctaaaga taacactccg atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc
    19441 aggacatcgt ttccaacgct tcctgcacca ccaactgcct ggctccgctg gctaaagtta
    19501 tcaacgataa cttcggcatc atcgaaggtc tgatgaccac cgttcacgct actaccgcta
    19561 ctcagaaaac cgttgatggc ccgtctcaca aagactggcg cggcggccgc ggcgcttccc
    19621 agaacatcat cccgtcctct accggtgctg ctaaagctgt aggtaaagta ctgccagaac
    19681 tgaatggcaa actgactggt atggcgttcc gcgttccgac cccgaacgta tccgtagttg
    19741 acctgaccgt tcgtctggaa aaagctgcaa cttacgagca gatcaaagct gccgttaaag
    19801 ctgctgctga aggcgaaatg aaaggcgttc tgggctacac cgaagatgac gtagtatcta
    19861 ccgatttcaa cggcgaagtt tgcacttccg tgttcgatgc taaagctggt atcgctctga
    19921 acgacaactt cgtgaaactg gtatcctggt acgacaacga aaccggttac tccaacaaag
    19981 ttctggacct gatcgctcac atctccaaat aagttgagat gacactgtga tctaaaaaga
    20041 gcgacttcgg tcgctctttt ttttacctga taaaatgaag ttaaaggact gcgtcatgat
    20101 taagaaaatt tttgcccttc cggtcatcga acaaatctcc cctgtcctct cccgtcgtaa
    20161 actggatgaa ctggacctca ttgtggtcga tcatccccag gtaaaagcct cttttgcatt
    20221 acagggcgca caccttctct cgtggaaacc tgcgggtgaa gaagaagttc tgtggttgag
    20281 caacaacaca ccgttcaaaa atggcgtcgc tattcgcggt ggcgtaccgg tttgctggcc
    20341 gtggtttggt ccggcggcac aacaaggtct gcctgcgcac ggttttgccc gcaacctgcc
    20401 gtggacgctg aaatcacatc gtgaagatgc tgatggcgta gcgctgactt ttgaattgac
    20461 gcaaagcgaa gagacgaaaa aattctggcc gcacgacttt acgctgttag cgcatttccg
    20521 cgtgggtaaa acctgtgaaa tcgatcttga atcacatggc gaatttgaaa ccacctctgc
    20581 cctgcatacc tactttaacg tgggtgatat cgctaaggta agcgtcagtg ggctgggcga
    20641 tcgcttcatt gataaagtaa atgacgcgaa agaagatgta ctgaccgacg gtattcagac
    20701 cttccctgac cgtaccgatc gcgtgtatct gaatccgcaa gattgcagcg tgattaatga
    20761 tgaagcgctg aatcgtatta tcgccgtagg ccaccagcat catctgaacg ttgtcggctg
    20821 gaacccggga ccggcgcttt cagttagcat gggcgacatg ccggatgatg gctacaaaac
    20881 atttgtttgt gtagaaacgg cttacgcttc agaaacgcaa aaagtgacca aagagaaacc
    20941 tgcacatctg gcgcaatcca ttcgcgttgc gaaacgttaa tttgcgttaa tgttgtgtgc
    21001 cgggtgcgat gcatccggca cgtatcaaca tcacaccata tccagcgcag tttttccttt
    21061 tggtgccgga tacgctttat ccagcatggt taattccgct gacgaaagtt cgacctcaag
    21121 cacagccgca ttctgttgga catgggcaat cgtggccgct tttggaatcg ccatcacacc
    21181 ctgatggctg attacccacg ccaacaatac ttgtgccgcg ctgatattgt gagcatgtgc
    21241 aatttcgttg actaccgcgt tttttaacag tccattgcgc aaccgcccgg cctgggctaa
    21301 tggactgtaa gccatcaccg gcatctgctg ttgctggcac caggggagta gatcgtactc
    21361 aatccctcgt gaaccgagat ggtaaagcac ctgattagtg gcacactgat ttccccccgg
    21421 cagctgccag agttcctgca tatcagcatg atcaaggtta gaaacgcccc agcggcggat
    21481 ttttccctgg gcgatcaatt tttccatcgc tgccacagtc tcttcaaaag cgaaactgcc
    21541 agaccagtgt aataagtaga gatcgagata atcagtattg agacggcgta aactggcttc
    21601 gcatgcattt atcgcttttt gcccgccagc attccacgga tagactttag agacgagaaa
    21661 gacgttatcg cgcagaccgg ttaatgcttc cccaaccacc ttttcggcac cgccatcggc
    21721 atacatttcg gcggtatcaa tgagggttaa accgagctca atgcccgcgc gtagtgcagc
    21781 aacttctgtt ttgcgctgac tggcatcttc gcccatatac catgttccct gccctatggc
    21841 tggcagtgag acatcgccac taaattgaat cattttttgt tgcattgttt cctcccaggt
    21901 attgcaccac cgtaatgcaa aacagggcgt gacgccctgt ttttatgcac aaaatgccct
    21961 ggaaagatgc attatcagaa tttgtaggtg atcccggtag aaatcaggcc agtccaggat
    22021 ttatccacca tcgggctgtc agtaacttca tcagacagac gggtatagcg cgcggtaccg
    22081 taaacactcc agtcgccgag gaagttgtag ctggcgctca gctccaggta agggctccag
    22141 ctgtcgttcg ggttatagcc acgcagaccg ctgcgagcgg actctttgcg cgatacgcca
    22201 taatagtatt cgttctggtt ttcgctgttc cactgcacac caatacccgg agtcacggtc
    22261 aggccaccgt tggtgtaacg atacaaccag gccatatccc agacaatgcc gttgctgtta
    22321 tccagggtat cgccagccag ggtggtacgc aggtaaccgt actgggtaaa gtgagcataa
    22381 gacagaccag ccatcatggt gctcttacgg tcatccaggt gacgcatttg gtgatcacca
    22441 ctgtctttcg ctttgaagta aagcggcgac cagtaagcgg taattgaaag tttatccgtt
    22501 gcgtcattcc acaggtagta accaccacct aagccacgga accagaagtt atcgccttca
    22561 tagttgatta ccggtactgg gtaaacatcg gtatcgtaat ctttatatgg gtgttcaacg
    22621 acacctacgc ctgcgcccag ggaaaattta ccttcagcgt gcgctacgcc tgcagacgtt
    22681 gcgataagca ctccaagtgc cagaagtttg agtttggtca caattaatca ttccttaaac
    22741 aaatgtttag cgggcgacaa agtttacccg tcaatacatc gaaacccaac ctttttacgt
    22801 tttcattttt taaagtaact gtttaatttt cctgacgcgg atgacaccgc gcttacagcc
    22861 aaatgaattt agcgttactg gctaccctgg tctttacatt aattatgcaa aatttatgga
    22921 tgagttgttg atatgccatt gaaattaaga aagccgtgca ggcaagtttt ccatttgcca
    22981 tctacgctta attttgaagg tgtatcaccg ggcacgttgt tctcatcgtc gataaaatgg
    23041 catgagagtt gctgtgtttt agcaagagac gtcgttcagt ttacctcttc cgggagcctc
    23101 tactattcat atgaacggct cttaacctgt gctaaaaaac gaaaggacgg cataccatga
    23161 atatattcga tcactatcgc cagcgatatg aagctgccaa ggacgaagag ttcacactgc
    23221 aggagtttct taccacttgt cggcaagatc gcagtgctta tgccaacgcg gctgagcggc
    23281 tattgatggc tatcggtgag cctgtcatgg tcgatacagc ccaggaaccc agactttctc
    23341 gactcttttc taaccgggtc attgcacgtt atccggcgtt tgaagagttt tacggcatgg
    23401 aagacgcgat tgaacagatt gtctcttatc tgaaacacgc ggctcagggg ctggaagaga
    23461 agaaacaaat cctgtatctg ctggggcctg tgggtggggg taaatcatcg cttgctgagc
    23521 gactgaaatc attaatgcag ctcgtaccga tttatgtatt gagcgccaac ggtgagcgta
    23581 gcccggtcaa cgatcatccg ttctgtcttt tcaatccgca ggaagatgct cagattctgg
    23641 aaaaagagta tggcattcct cgccgttatc tcggcaccat catgtcgcca tgggcggcaa
    23701 aacgcctgca tgaatttggt ggcgatatca ctaagttccg ggtagtgaag gtctggccgt
    23761 caattctgca acaaattgct atcgccaaaa cggaacccgg tgatgagaac aaccaggaca
    23821 tctccgcgct ggttgggaaa gtcgatattc gtaaactcga acactacgcg cagaatgacc
    23881 cggacgccta cggctattcc ggtgcgctgt gccgcgccaa ccaagggatc atggagttcg
    23941 ttgagatgtt taaagcaccg ataaaagtgc tgcatccgct gttaaccgcc actcaggaag
    24001 gtaactacaa cgggacggaa ggtatctccg ccctgccgtt caacgggatt atcctcgccc
    24061 actcgaacga atccgaatgg gtcactttcc gtaataacaa aaacaacgaa gcctttctcg
    24121 accgtgttta catcgtgaag gtgccgtatt gcttgcgcat ttccgaagag atcaaaatct
    24181 acgagaaatt gcttaatcac agtgaattga ctcacgcccc atgcgcccct ggcacgctcg
    24241 aaacactgtc acgtttttcc attctttcgc gcctgaaaga gccagaaaac tccagcattt
    24301 attcaaagat gcgggtttat gatggcgaaa gtctgaaaga caccgatccc aaagccaagt
    24361 cgtatcagga atatcgtgac tacgccggtg tcgatgaagg gatgaacggt ctgtcgacgc
    24421 gttttgcgtt taagatcctc tcccgcgtgt tcaacttcga tcatgtagaa gtggcagcaa
    24481 acccggtcca tctgttctac gtcctggaac agcagatcga gcgcgagcag ttcccacaag
    24541 agcaggcaga acgctatctg gagttcctga aaggttatct gatcccgaaa tatgccgagt
    24601 ttatcggcaa agagatccag acggcctacc ttgaatccta ttccgaatat gggcaaaaca
    24661 ttttcgaccg ttatgttacc tacgcggatt tctggattca ggatcaggag tatcgcgatc
    24721 cggataccgg gcagctgttt gaccgcgagt ctcttaacgc cgagctggag aaaatcgaga
    24781 aaccggcggg gatcagtaat ccaaaagatt tccgcaacga gattgttaac ttcgtactgc
    24841 gcgccagagc gaataacagc ggacgaaatc cgaactggac cagctatgaa aaactgcgca
    24901 cggttatcga gaagaaaatg ttctccaata ccgaggagct gttgccggtt atctcgttta
    24961 acgccaaaac gtcaaccgac gagcagaaga aacacgacga ctttgtcgac cgtatgatgg
    25021 aaaaaggcta cacccgtaaa caggtgcgtt tactgtgcga atggtatttg cgcgtacgta
    25081 aatcgtctta acaaccctgg cccggtacac atgctaccgg gcctacaacg acagcgaacc
    25141 gtgggccaga gaaatggcaa cacaggcgta gcatacagtt ggcaaatgta gtacgggggg
    25201 catatgacct ggtttattga ccggcgtctg aacggcaaaa acaaaagcat ggtgaatcgc
    25261 cagcgttttt tacgccgtta taaagcgcaa attaaacagt cgatctccga ggccattaat
    25321 aagcgttcgg tgactgacgt cgacagcggc gaatccgtat ccattcccac ggaagatatt
    25381 agcgaaccga tgtttcatca ggggcgtggc ggtctgcgcc accgcgtgca tccgggcaat
    25441 gaccatttcg tccagaacga ccgaattgaa cgtcctcagg gtggcggcgg aggttccggc
    25501 agtggtcagg gccaggccag ccaggatggt gaaggtcagg atgaatttgt ctttcagatt
    25561 tcgaaagatg agtatcttga tttgctcttt gaagatttgg ccttaccgaa tctgaaacaa
    25621 aaccaacaac gccagctgac cgaatataaa acgcatcggg cgggttatac cgctaacggc
    25681 gttccggcaa atatcagcgt tgtgcgctcc ttgcagaact cactggcgcg acgcacagcc
    25741 atgacggcag gcaagcggcg ggaacttcat gcactggaag agaatttggc catcatcagc
    25801 aacagtgaac ctgcgcagct gctggaagag gaacgtctgc gcaaagaaat tgcagaatta
    25861 cgtgccaaaa ttgaacgcgt cccttttatt gacaccttcg atttacgtta caagaactac
    25921 gagaagcggc ccgatccctc cagccaggca gtgatgtttt gcctgatgga cgtttccggt
    25981 tcaatggatc aatccactaa agatatggct aagcgttttt atattctgct gtatctgttc
    26041 ctcagcagaa cgtataagaa cgtggaagtc gtatacatcc gccatcatac ccaggcgaaa
    26101 gaagtcgatg aacatgagtt tttctactcg caggaaacag gcggcaccat cgtttccagc
    26161 gccctgaaac tgatggatga ggtagtgaaa gagcgttata acccggcaca gtggaatatt
    26221 tacgctgcac aagcatcgga cggcgataac tgggccgatg actctccgct ttgccatgaa
    26281 atcctggcga aaaaattatt acctgttgtt cgttattaca gctatatcga aattacccgt
    26341 cgtgcacatc agacattgtg gcgagaatat gagcatctgc aatctacttt cgacaacttt
    26401 gcgatgcagc acatccgcga ccaggatgat atttatccgg tgttccgtga actgtttcat
    26461 aaacaaaatg caacagctaa agactaaaac tatcagccag gtcattatcg cctggctgat
    26521 ttttagctta ctgtaaatta tctcatttat taca
//
LOCUS       DAMZII010000065        26451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZII010000065 DAMZII010000000
VERSION     DAMZII010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.065"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            101..712
                     /locus_tag="POA77_002602"
     CDS             101..712
                     /locus_tag="POA77_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6366006.1"
                     /translation="MLRQFFLLTATTFLCGCGYHFGNDVDAYDLLPRPVGSKKFEIVA
                     PDDSIQSRMFAARFASGLTGKGFNISSHQPEYILRFSYSKTQENLQYSELPVTGITGY
                     VIEKKTTRKDKHGQTETDYDYKPVSGIVGTETVSQRHFWRRLDVEVYPAGKNAQQVLK
                     VSMQSNAPIPSDSVAYSAMIDALTGNLDAPLRSGNYVASVPWN"
     gene            complement(771..1193)
                     /locus_tag="POA77_002603"
     CDS             complement(771..1193)
                     /locus_tag="POA77_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366007.1"
                     /translation="MSLWLSIVTRATGLPDKRYLNDPVILRYYQQKIQKAPYALVGWT
                     LCFMATIGVSLGLDLSNQMLLLVLLILIFLMVVLQGRYQFMHPERCINAKFYQTVLDD
                     ASVSDIQKAQFEYLFVTKVDELTIGDLYRLYDFNRGGR"
     gene            complement(1216..1608)
                     /locus_tag="POA77_002604"
     CDS             complement(1216..1608)
                     /locus_tag="POA77_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366008.1"
                     /translation="MKSAILTTLMLVAVGSQPVFAAKCQFGACDADTVASSEILVSAA
                     QTDKDAVLKKLNDATSRSDVMSKRFESYLSNDRLKVNTDSTKKGPSNEIAKPRDELAP
                     VRQLKAIRPELVQTPIFQPIVAFYERLA"
     gene            complement(1634..2383)
                     /locus_tag="POA77_002605"
     CDS             complement(1634..2383)
                     /locus_tag="POA77_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HDI6366009.1"
                     /translation="MNKTIKASLFLLASCTALSMNAQTANTDVLPDAALKKLREVGLS
                     IEHIEPSPVKDIYTVISREGVSYVSKDGDYIFTGSLFHVNGKDVENTTEQAILKGVRE
                     FATKTKSIEYKSANEKYRLAVFTDITCGFCQKLHQDLQSYLDAGISIKFLAFPRAGLN
                     SVVAGNMAKIWCAAKPNDALDAAMSPTSTIPEGRPDEACLDTIKSHFQVASTIPLQGT
                     PTMVTLSGKPQLFTGWMSPENLVTRMGAEQK"
     gene            2531..3046
                     /locus_tag="POA77_002606"
     CDS             2531..3046
                     /locus_tag="POA77_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6366010.1"
                     /translation="MFIPPVDDVKPIPVPVEIYTQCITDASRFFGIDAELVFTLFDNE
                     GGKVGTFSRNKNGTYDIGPMQINSSNLPEIRDHFPSVTWRVLAYDACASFWVGTWWLY
                     RKIVDRKGNVFEGIADYNSKTPKVRATYIFNFMIKYNRRIQRRNGMDELYQWTQPKPQ
                     YNGHIVKNLPE"
     gene            3143..3709
                     /locus_tag="POA77_002607"
     CDS             3143..3709
                     /locus_tag="POA77_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366011.1"
                     /translation="MLQSIYKFLFNLCSSPQLPKNKDVDGILSHEEITAGLWGPLPSI
                     HVVLFLDFDGVVHKCQNESFERMHLIERLLDASPSMFIVISSSWRECGSITYLKSLFR
                     SPYRDRVIGATPSLYLPSGSIGVRAAECEDFVTRHKVKAFICLDDDATLFPPEYPHLF
                     RTDYYTGVNEKDLTDLIARYNVLMTRFY"
     gene            complement(3956..7945)
                     /locus_tag="POA77_002608"
     CDS             complement(3956..7945)
                     /locus_tag="POA77_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDI6366012.1"
                     /translation="MDYNIYTVGDIEFVWSALNGIALIFSQYTGVKAFLTTAAVLAGA
                     SLFYKTWLWLLNPTKAEVPIFSWILGLILFSMAIVRVDVTIESVKSGEVRNVDGIPIF
                     IAAMGTVTTNLSQGLLKDYKTAFDPLAPIDFAATTLDDDITLGPMIRFVKFLQWGGDS
                     QGYCSAFPEPASGLGAMNVCATVQSLAYNCLKGTQNSSAKIAGKETIFNDIFSANIVD
                     SMERINQAMKGSLKNASANIVGVNSSKSATCEEVWSTVKQVTSTPEARQTIALIGQTN
                     GILAPDEAGGAASGASFTDVMASANGMYGKAIGAYDATLSLFIMNELRNGASKYKTPL
                     GLASDMQLFEASLKRTNTMASQGQLWLQLSGAAIAFLEMFAYMVAPFALLMLLALGGN
                     GVAAAAKYLQLILFVNMWPITAVMVNAYVKKVATADLDTWSTLNSQSNAVTWMGLPGL
                     AETYSSYLSVASALYALIPVLTLFLMTQSIHPMMNAMKGVTPPAPVDSSHLTPKVWDA
                     PNSGKSSFGDVNRTALTSTGQGFSGGGAMDSSNFRLGTWNAGSSIASSQGQGAAFTSS
                     AMSAASNTFQQAFNQMSEVGRTGQSGQQFTSNLQSLKSFTDQAGANIANSVGAKHGIN
                     SQQLAGVVSNLLLNGSLGLGGEEKLGKGGGGGLAQKVMDSLGIKAGGQVGSGITKSNS
                     GNESLLTDLSKAITTQLTENSGLTDQMTKAAAQINSDQFAQTNAFKEAATKMNQSTQT
                     MAENLSTSLSTNASANTGMTLDSRQAINLDRFSDSIRNKNFSDEDVRNFARKNGLDEN
                     AFMEKFNSYNDTFKASTQLGSQLQRTDALVATTRDFSEQKIAIDTARGETAESNKQDL
                     RETSSLLKGLVADFGGNAQQMLPITNQLDRITGDASGINTIAQAQDRTPEHVNTSSVM
                     SADRVADVGQSMDAQAKAGLVQNQQNANQHVGGQTESGVTPYNVDKISKEDIQGIHQG
                     NVNKSYSEENQNVLSSLEKNTPVVTNETVGRVQGAGVDVQHANQTFDQLQAGSGGKHA
                     TSGMGTISDKLNNMYGDNQIRGISGNLPNYFGRIANDPTKKPDDKRAELAAQAVFTYG
                     ASTMATGAEKEQLKADTQNILSELSHYNVRWGMNDLQNIHEGFNMHNKASGSLDSVVR
                     ANLGEGGSGGGLVGQRTQTTTDLLVGNKIESNTERGVIGSTLLSGQESVARGLDVIGA
                     KPVNDMLSGMGILTTPSSLANDASNPQNMPSSLLGKVGNHLQMSDGVSEVTSRYKDMG
                     GDGIKAYETASQNSERAIRQQLTDDPRFGPDKADAFVSFMKSNLNNTNEPYQSRIEKA
                     KSWLNENKK"
     gene            complement(7954..9369)
                     /gene="trhH"
                     /locus_tag="POA77_002609"
     CDS             complement(7954..9369)
                     /gene="trhH"
                     /locus_tag="POA77_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhH"
                     /protein_id="HDI6366013.1"
                     /translation="MRADFHVCAKKLLLSLAVCTAMIAPNAFADNAMRNIFNGMMTTT
                     SPASFETATRTGVVGGSFSYRTTNVNTNLVSMSFPKASVGCNGIDVFLGSFSMINGDQ
                     LVQVARGIAQGAAIYAFNVAVSAICADCAATINDIQNKLQALNKFAKDSCNATYSFLT
                     ENVGPPSQFANAVSSGPASMLGTLNGLLPDFGSSMTKSPETVTSQVKAKDPEEFAEKF
                     SGNLFYMSFMDIDKGSMNIGGVTELSGYRLAEQLMSLVGTVIINWDAKGEKAGMEVRP
                     STMTVSDFIMGPPAGGSIKMLKCSPAPDPASARKSQCLVMSEVTDGGFKGLKDTISDL
                     LIQVQTKAISDVRISDDELRIISYIGIPTILDSLQTFDVPEGYAYIQDISAIAATSLV
                     INMLRQVEAKISAMNIPSESLSGRRQDLTRLSENLTNQVKAAYELTRSQVGNSSDVIS
                     AWDDRRLKRKAFMESLRGTGN"
     gene            complement(9359..10405)
                     /locus_tag="POA77_002610"
     CDS             complement(9359..10405)
                     /locus_tag="POA77_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6366014.1"
                     /translation="MTRLTLIPSKMLVLLSLFITGVHANEEQALIKDTPFVSGQAFKK
                     GFFWYDDPTRKTEEEITETKPPVASSTQPEQEEKIELNSKWLKDNMPQLLTKAMDNPT
                     PENLSRFYTAQRLMLDIGTRFSDKSKDYFLKNPMMSEKRRQPVEKVALDAHRTVVEKN
                     QQSVMKDIFTKSGLFFFFQSTCQFCHEESQILQFMENYYSVDILPVSMDGRPLQNGLF
                     QDFSVPNAQIIDQFKIREVPTIFLVSKDGSSAQRISEGMITAEELKNTIILAAKGMKL
                     IDDASFQSTLDIKRQYTIGEDGVITVNKSEMDSDPFLLQRIMDKKLEGYDMPTADPVN
                     YLNVGGSLGGAYAR"
     gene            complement(11017..11529)
                     /locus_tag="POA77_002611"
     CDS             complement(11017..11529)
                     /locus_tag="POA77_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366015.1"
                     /translation="MAKVDQTKANMLLSATKTMSLGANKNSIRILYGNHVAENIDFRS
                     EPQQNFKKGIRGWSRRTDNRRAFSAVIDVAVNYFGFDPIHPESSFRNIDFNRIMSVLH
                     NLGYFVCAPEVNKIIEILTSMKEIEELETCAKCKRPYIVPYTNDTHCPRCRLSSTSSA
                     IARYSQEELD"
     gene            complement(11532..12332)
                     /locus_tag="POA77_002612"
     CDS             complement(11532..12332)
                     /locus_tag="POA77_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trhR"
                     /protein_id="HDI6366016.1"
                     /translation="MSESDNITRNLLGKLLRTEISIIRSYRAFLMLLPLHGSSKYQTG
                     SPLLQRRLFGNGFGAMIDNAFEVETRPGSFLVPRSLSKEISWDKFFVAVVDGDTNVIR
                     EYDSEDTDFGIYNEGEKVTLLSGQEEFYNPRKIQQLRSKCVDIQNDYLMQVFFMSMLA
                     PEFVSIFFGLKPATVEAIKDVGMSSLKLINDIVLFPRTIPFTPGLGDDSATLKSKVFA
                     WAYEISADIRLGKVSDDLMELLRYDTMFTCHRQDVFNTLANKVLLKDY"
     gene            13090..13575
                     /locus_tag="POA77_002613"
     CDS             13090..13575
                     /locus_tag="POA77_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDI6366017.1"
                     /translation="MSKSKLLKSLEISRTLLRSQIDVLREEAEELITNYWIEWKERNH
                     IERNLPRDSKTLKKEYLGSYAPKVELIGNARKVTITWHQFSPYKNRPPSHMSKRISPM
                     KSGKYTKSCFVNHASWEYEMIEKTEGLLEPYRELLEFYHAAYIELGRKVRQYSKSEVA
                     Q"
     gene            13572..14294
                     /locus_tag="POA77_002614"
     CDS             13572..14294
                     /locus_tag="POA77_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366018.1"
                     /translation="MTDVTMNESEHKVPDVNTRSPHASDNSRRVVQSKNEHYGMATVT
                     ITPGTPDFNRFLTARNRSVIRGFDDVSIAISSLFRTVNAVKHPELVQAIQDWFNELHE
                     ENNLMKNNLERHIATIHIDESDPFFSSTEFSPFRFEPVQLNFNNQNTMRFYKHIFEMN
                     NLLTQMHKFNSLGQLPVSDYNVMAHNIIRSLNMYIERVKKTLNVSRRAKGAYSPDEFI
                     EKVKQYKSVQAYIAAELSGKRR"
     gene            14305..17340
                     /locus_tag="POA77_002615"
     CDS             14305..17340
                     /locus_tag="POA77_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDI6366019.1"
                     /translation="MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGE
                     QKYTVPDHPNPEVLKFIEYPTRPAGIQTFNEQSILSLYRDKLHSISMMLAISDGDIRE
                     DAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELR
                     PIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPSSQSLLDWA
                     RENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNV
                     YNDGNDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININN
                     YKTKPMHIIIVNGEVYLNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVE
                     PYDDERVVHYFIPGTYSENEIASIFRNGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPG
                     LFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVNDAIENTPQYGLQLVNGPD
                     ADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQVEEHDD
                     RSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQL
                     TYRDGSLDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDI
                     PEAPSVNEAASPPQTPLELPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGH
                     LLEETLDSDGEAYSDLIASDSTEAEYPATDPQSSDFAQLPRETALSVAPGDLDYSEGA
                     IKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALARLFAVSTHAEKKHEKT
                     QEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEIATML
                     HQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSG
                     AVVNTKCIAFNIPTLVAASDRAKVDIELIINKLKEVGNL"
     gene            17340..19424
                     /gene="traD"
                     /locus_tag="POA77_002616"
     CDS             17340..19424
                     /gene="traD"
                     /locus_tag="POA77_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284076.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HDI6366020.1"
                     /translation="MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDG
                     VNIAPIILYTAVFLLLVCLYRCKTAHPYLMAHWRVFQRQIMFISLKSLRTINKSNFFS
                     NERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHADRAYQIANLSSDKREIALPFV
                     LSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTGKTVLQRLL
                     SISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYT
                     NVSDLTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMS
                     VVNLTIKVMECCFARHYGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPI
                     EWIDTVGRLVDYSMSDPEHMSKMTAGIMPLFSRLTEQPLNELLSPSPNTLTSREIVTS
                     DGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGSRYNADDGDMSSHSRISIF
                     VDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLCNNYISL
                     RVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPK
                     ELLGQVPKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVG
                     LSYLNKYLRKLH"
     gene            19424..20527
                     /locus_tag="POA77_002617"
     CDS             19424..20527
                     /locus_tag="POA77_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366021.1"
                     /translation="MDEQITLTQNQIFSASLKVSKSRSLVKRRMQSLGLKFTESQDVR
                     NRLAGIEKGALKCVGQFCHDNDAESLSAMAIILSELFLLQGELSTSNEYGDHETSYWT
                     VAQGPCDEWVQSLLASENGRRTFNSFRITFDNSEERRSLVEKNAKMLGSYLLPYFVNF
                     TNAASAFITLPNSITFKQVQRNKPLIHPETTLSHILTIEDSAFLSRIKFKLISAIDRL
                     PDPSGQYANMFNHIMDRALLTHLNREQIDSPCVCKKVISTYADTMLTLPIFNTTITGK
                     YRHWTPWGINFVEFSRQAAKEKSCVYVPEPGQIHWKSPEHKELAEYSLINQIIPQQYH
                     WLLGVPTIWRSHYRDHSKRLDLFKEWRDANGCG"
     gene            20514..21176
                     /locus_tag="POA77_002618"
     CDS             20514..21176
                     /locus_tag="POA77_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HDI6366022.1"
                     /translation="MAADNSARAIFIRGLLLLGMMLYLIYSLGFQNTDELKQQITQEV
                     NASRSLISNDRWLSVIANSEATLSTLINDYKLIDYLNTILIPDTSTPARGMNYVAEKM
                     TSVNYTVAKNLPLLIYQSIFRWNLILGWLILFSPYLIAMLADGMYQWKLKRYVFGNVT
                     VQFYRIWFRAFWIIGALTFIYLSMPNMSLFNNIAQLFPPVALLILGIALNRLWSNFQK
                     LM"
     gene            21189..22370
                     /locus_tag="POA77_002619"
     CDS             21189..22370
                     /locus_tag="POA77_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDI6366023.1"
                     /translation="MFKRNNKKSPANIKESLDENADRFYKMFRIHTTSKVVISLLALA
                     AAGVSAFNIYDKYQESEGRMDHIAVVRISGAMGTGSEVGDGSVIATALAKAYNNPHAK
                     AVIIEAESGGGGPSDAIIIYRQINALRSHQQKIERVSHAESTSKPRNTSDHSASRSQA
                     DSNAELSKRNTLDVLSAGSGHFISDMENSYKPIIVSVKGICASACYYAVSPADAIYAD
                     SSALTGSIGVRMDHWNLSRVMDTVGVKNEPLTAGEFKDALDPYHPLSDATRDFMQKQI
                     LDAMHEQFIADVEQGRGKKLLSRPEADAVALYSGRVWTTQQAIRYGLIDGDLTPVEVR
                     SRLSQMYSTTIFKTYNEPQRSLRSALGMLMSLSTNVETLAGTTSRILDSVQATSYPTV
                     R"
     gene            22372..23100
                     /locus_tag="POA77_002620"
     CDS             22372..23100
                     /locus_tag="POA77_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDI6366024.1"
                     /translation="MDAILMVWRVIGISIIVLALIVLYCLMGFVSSAIAERRTARQIE
                     IYLPDDAEGLLSDLTLPVNATGTTQIDHVLIASHGLYVIEQKNYAGKLYGKLEESHWR
                     KWKSSGTLKLQNPFRQNYGHIRAIQSTLRASELECINVVIINGPCKFEGEKPDWLCMG
                     MEEFVRKVTERRQLNVFKPEAVSFIRGELKLKRKPPGLYTDLNHIHNVTTRYKTTMRL
                     EQRITYNLLRFSRLILSKMFRSAK"
     gene            complement(23127..23483)
                     /locus_tag="POA77_002621"
     CDS             complement(23127..23483)
                     /locus_tag="POA77_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366025.1"
                     /translation="MTTANVTTTTYRSDDIVPFRRPQGDLDNRYMPQAYALVRSWASK
                     PAEYGAGVLATYRQPVVNLGYKIKGTRVVLILVPIECEPVGVKMTDAALWPSLSIGEA
                     MRIMQEAWQNIPELNP"
     gene            complement(23570..23884)
                     /locus_tag="POA77_002622"
     CDS             complement(23570..23884)
                     /locus_tag="POA77_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366026.1"
                     /translation="MNTFLYVLVGWIIVLYLVNKVREKYSKPKTVNILVKRNGVYQEV
                     EAVVMPRSHNSDVGSGKTGNEAAPQWLNKEDDISELEYYSGGSLLKAARDFEAQQMEQ
                     KF"
     gene            complement(24808..25044)
                     /locus_tag="POA77_002623"
     CDS             complement(24808..25044)
                     /locus_tag="POA77_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDI6366027.1"
                     /translation="MSDLFSSESPVTLAQARTVAAGYQNVFIENLQPAGHFQIVIRDH
                     RDHDSQLVWRNWNYESGANDALNSYLQSHGLKAS"
     gene            complement(25090..25635)
                     /locus_tag="POA77_002624"
     CDS             complement(25090..25635)
                     /locus_tag="POA77_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366028.1"
                     /translation="MKTLNIQVNDIAAISALGGSMLADIIAAQYKVIDDTKDASEMAM
                     PCIAYGQIPANIGPGVFINRVYSMDSQSLSASQRYELFNGLATQAFTFFFQSGWYGAF
                     RVVSFRDNGPAGLLNIGIVHDKVLNEFCDDFPRANVSETGFNCGMTFDQIRMFISDLA
                     YQMPLYNNQDHFVKLAKHLAA"
     gene            complement(25716..26153)
                     /locus_tag="POA77_002625"
     CDS             complement(25716..26153)
                     /locus_tag="POA77_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366029.1"
                     /translation="MYKYDRNRQTFNLINALQPVYRNNGGGLFEFDRRKQHETADLIF
                     EALTFSPTPELELAKFVRLKSVRRIAQFDDGEVRYGVHVELDEAVNINSFRIFKDDNT
                     GGFDFYLFSVLGNNAELVQINLDASNIHLQDIFERSTGLCLSY"
     gene            complement(26203..26451)
                     /locus_tag="POA77_002626"
                     /pseudo
     CDS             complement(26203..26451)
                     /locus_tag="POA77_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856245.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7269 a         6235 c         5849 g         7098 t
ORIGIN      
        1 ctttacgcat ttcaacttgt tagttatcag cctgcgttcg attatactgg cacggtaaat
       61 cggaattatt cattaaagaa ccctttttgg aggatttaga atgttacggc aatttttctt
      121 actaacagcc acaacgttct tatgtggatg cggttatcat tttggaaatg acgttgatgc
      181 gtacgattta ctgccccgcc cggtaggcag caagaaattt gaaattgttg ctccggatga
      241 cagtattcag tcgcgaatgt ttgcagcccg gtttgcgagc ggattaacgg gtaaggggtt
      301 taatatttcc agccatcagc ccgaatatat tctcagattc agttacagca agacccagga
      361 gaatctgcaa tacagtgaac taccggtgac gggaataacc ggatatgtta tcgagaagaa
      421 aacaacgcga aaagataagc acgggcaaac tgagacggac tatgactata agccggtcag
      481 tgggatcgta ggaacagaga cggtatcaca aagacatttc tggcgtcgtc ttgatgtgga
      541 agtctaccct gcgggaaaaa atgcacagca ggtgctaaaa gtcagtatgc agagcaatgc
      601 gccgatccca tcggacagcg tggcgtattc cgcaatgatt gacgctttga ccgggaatct
      661 ggatgccccg ctgcgctccg gaaactacgt cgcctcggtc ccctggaact gatgaccggc
      721 actgtaacga ccaaccacaa attaactttt ctcacctgca tgccacgtct ttaccggcct
      781 cctctgttga aatcatagag tcggtacaga tcaccgatgg tcagctcatc cactttggtc
      841 acgaagagat attcaaactg agctttctga atatctgaga ctgacgcatc gtccagcacc
      901 gtttggtaaa atttggcatt gatacatctt tccggatgca tgaactggta ccgcccctgg
      961 agcaccacca tcagaaagat caatatcagc agaacgagaa gtaacatctg gttggaaaga
     1021 tccagtccca gcgacacacc aatggttgcc atgaaacaga gagtccatcc caccagggcg
     1081 taaggagcct tttggatctt ttgttggtaa taacggagga tcaccggatc atttaaatac
     1141 cgcttatccg gcaatcccgt tgcccgggtg acaatgctga gccaaagcga catcttctaa
     1201 cccccagcga cgcactcagg ccagacgttc atagaacgca acaatgggct ggaagatggg
     1261 tgtttgtacc agctcggggc gaatggcttt cagctgcctt accggcgcca actcatccct
     1321 cggcttagcg atttcattgc tagggccttt cttggtactg tcggtgttca cctttaacct
     1381 gtcattgctc agatacgact caaaccgttt cgacatcacg tcgctgcggg atgtcgcatc
     1441 attgagtttt tttaaaactg cgtctttatc tgtctgagca gcagatacca gaatctcact
     1501 gctggccaca gtgtcggcgt cgcaggcccc gaactggcat ttggcagcaa agacgggttg
     1561 agaaccaacc gccaccagca ttaacgttgt cagtattgca gatttcataa acacctcttg
     1621 cagtcgtgga aagtcacttt tgttcggcac ccatgcgggt caccagattt tccgggctca
     1681 tccaaccggt gaatagctga ggtttccccg acagcgttac catcgttggc gtaccctgaa
     1741 gcggaatagt tgaagctacc tggaaatgcg atttgatggt atcgaggcag gcctcgtcag
     1801 gccgtccttc cggaatggtt gatgtcgggc tcattgcggc atccagtgca tcattaggtt
     1861 ttgccgcaca ccatattttt gccatattcc ccgcgacaac ggagttgaga cctgcacgtg
     1921 gaaaggcgag gaatttgatt gagatcccgg cgtcgagata ggattgaaga tcttgatgca
     1981 atttctggca aaagccgcat gtaatatccg taaacactgc cagacggtat ttttcattcg
     2041 ccgacttata ttcgatggat tttgttttag tggcgaactc cctgacacct ttcagaatag
     2101 cctgctcagt tgtgttctcc acatctttcc cgttcacatg aaacaagctc ccggtgaaaa
     2161 tgtaatcgcc atctttgctg acgtagctca cgccctctct tgaaatgacg gtataaatat
     2221 cttttaccgg cgatggttca atatgctcga tagacaagcc cacttctctt aatttcttca
     2281 gtgcagcatc gggaagcacg tcagtgttcg ctgtctgggc attcatactc aaggccgtac
     2341 aggatgccag taaaaataat gatgccttga tagtcttatt catttcagct cctttaatgg
     2401 agtgttaata ctatcatgcg aaaaaatctg cctaattcgc ttaaggggaa aaattccccc
     2461 ctatagcaaa aaaaattttg acgacgatat gggagaatat ccagaacgta attccagcgg
     2521 agattatctc gtgtttatac caccagtaga cgatgtgaaa cctattcccg ttcctgttga
     2581 gatctacacg cagtgcatta ccgatgcctc ccgttttttc gggattgatg cggagttggt
     2641 ctttacgctt tttgataatg agggcggaaa agtcgggacg ttcagccgta ataaaaatgg
     2701 cacttatgat attgggccta tgcagatcaa ttcgtccaac ctgccggaaa tacgcgacca
     2761 ttttccatct gttacgtggc gggtgctggc ctatgacgcc tgtgccagtt tctgggtggg
     2821 cacctggtgg ctttaccgga agattgttga ccggaagggt aacgtatttg aagggattgc
     2881 cgattacaac agcaaaaccc ctaaggtccg ggcgacgtat atcttcaact tcatgatcaa
     2941 atacaatcgc cggattcagc gccgaaatgg aatggacgaa ttatatcagt ggacgcaacc
     3001 taagccccag tacaacgggc atattgtaaa aaatcttcct gaataaaata cgaccctact
     3061 ctccaatttt ttattagttg tttcgtagcc ataaacaatg cggctcttag agaatgaaaa
     3121 cgtcctgaga aaaaggttaa cgatgctcca gtcaatttat aaattcctgt tcaatttatg
     3181 cagttcaccg cagctgccca aaaataaaga cgtggatgga atattgtctc atgaagaaat
     3241 aaccgctgga ttatggggtc ctctgccatc aatccatgtt gttcttttcc tggattttga
     3301 tggtgtagtc cacaaatgtc agaatgaatc gttcgagagg atgcatttga ttgagaggtt
     3361 acttgacgcg agtccttcaa tgttcatcgt tatatcaagc tcatggagag agtgcggcag
     3421 tatcacttac ctaaagtcac ttttccgctc gccatacaga gacagggtaa ttggagcaac
     3481 cccatccctg tatctcccct caggaagtat aggagtccgt gcagctgaat gtgaggactt
     3541 cgtcaccaga cacaaagtaa aagcctttat ctgccttgat gacgatgcga ccctgtttcc
     3601 tccagagtac ccgcacctgt ttcgtacaga ttattacacc ggggttaatg aaaaggatct
     3661 aacggacctg atagccagat ataatgtgct gatgacccgc ttttattgaa aaagtaactt
     3721 ttccacaccc tccatacaac caaagcctct gatgcgctat ggcctaggca ctaaagcatc
     3781 agtcgattca gaaaagttac ttttctctca attttcattt tgaacagaag aaatgcacat
     3841 tttcttagtg tgccgggtga cctgtatttg gtgaggaaat ttgaatcagg gtgatgcaag
     3901 caaaaaaaga ccgtactcaa aagagagtac ggcctttatc agctactact tacttttact
     3961 tcttattttc attcagccag cttttagctt tctcaattct cgactgatat ggttcatttg
     4021 tattattcag gtttgacttc ataaagctca cgaatgcatc tgctttatcc ggcccaaaac
     4081 gtggatcatc ggtcagttgc tgacgaatcg cacgttctga gttttgtgat gcagtctcat
     4141 acgccttgat cccatccccc cccatatcct tatacctgct ggtcacctcg ctaacaccat
     4201 ctgacatttg caggtgattt ccaaccttgc ccaatagcga gctcggcata ttttgtggat
     4261 tcgaagcatc gtttgccaaa gatgaagggg tggtcaggat acccatacct gagagcatgt
     4321 cgtttacagg tttagctccg ataacgtcaa gtcctctggc aacactctcc tgccccgata
     4381 aaagcgtact accaataaca ccacgttctg tattggactc aattttatta ccaacaagga
     4441 gatccgtcgt ggtttgggtt cgttgcccaa cgagcccgcc gccagaacca ccttccccaa
     4501 gattcgccct cacaactgag tcaagagaac ctgaagcctt gttatgcatg ttgaaacctt
     4561 catggatatt ctggagatcg ttcatccccc agcgaacgtt atagtgactc agttcggaga
     4621 ggatattttg agtatccgct ttcagttgtt ctttctcagc gccggttgcc attgtactgg
     4681 cgccataggt aaacacagct tgtgcggcca gttccgcacg tttgtcatcc ggctttttgg
     4741 taggatcatt tgcaatcctg ccaaaataat tcgggaggtt gcctgatata ccgcgtatct
     4801 ggttatctcc gtacatatta ttcagtttgt cgctgatagt acccatgccc gaagtcgcat
     4861 gcttaccgcc cgaaccagcc tgtaactggt cgaaagtttg gttggcgtgc tgaacgtcca
     4921 ccccagcccc ctgaactctt cccacagttt cattcgtcac aacaggggta ttcttctcca
     4981 atgacgagag cacattctgg ttctcttcgc tataggattt atttacatta ccttgatgaa
     5041 tgccctgaat atcttctttg ctgatcttgt ccacattgta cggagttaca ccagactcag
     5101 tttgtccacc aacatgctgg ttagcatttt gttggttttg taccagcccg gcttttgcct
     5161 gagcatccat actttgcccc acgtcagcaa cacgatcagc gctcattacg ctggacgtat
     5221 ttacatgttc tggggtgcga tcctgcgcct gtgcaatcgt gttaataccc gaggcatcac
     5281 cagtgattcg gtcaagctga ttagtaatcg gcagcatctg ttgagcgtta ccgccaaagt
     5341 cagcaaccag ccctttaagc aggctggaag tttcacgcag atcctgttta ttgctctcag
     5401 ccgtttcacc tcgggcagta tcaatcgcta ttttttgctc actaaagtca cgtgtcgttg
     5461 ccaccagcgc atctgtacgt tggagttgtg aacccaactg agtgctggcc ttaaatgtgt
     5521 cattatagga attgaacttc tccataaagg cattctcatc aagaccattt ttccgtgcaa
     5581 aattacgcac atcttcatcg ctaaagtttt tattacggat agaatcggag aatctgtcca
     5641 ggttgatagc ctgtcttgag tcgagtgtca tgccagtatt ggcgctggcg ttcgttgaaa
     5701 gtgacgtcga caagttttcg gccattgtct gtgtggactg gttcattttt gttgcagctt
     5761 ctttgaatgc attggtctgt gcaaactgat cactgtttat ctgagcagcc gctttagtca
     5821 tttggtcagt cagtcctgag ttttcagtca attgagtcgt aatagcttta ctcaaatcag
     5881 ttaagagact ttcattccca gaattacttt tcgtgatccc tgatccaact tgcccccccg
     5941 cttttattcc taggctatcc attactttct gagcgagacc tcctcctcct ccttttccaa
     6001 gtttttcttc accaccaagt cccaaactgc cattgagcag aagatttgac accacgccag
     6061 ctaattgctg cgaattaatt ccatgcttcg cccctacgct attcgcgatg tttgcaccgg
     6121 cctggtcagt aaatgatttc aagctttgca ggttggaggt aaattgctgg cctgattgcc
     6181 ccgttcggcc aacttcactc atctggttga aggcctgctg gaatgtgtta cttgctgcgc
     6241 tcatggctga agaggtaaat gctgcccctt gcccctggct tgacgcgata cttgaaccgg
     6301 cattccacgt accgagtcgg aaattactgg agtccatggc cccgccacca ctgaatcctt
     6361 ggccggtgct tgtcagcgcg gtacgattca catccccaaa cgatgatttt ccgctgtttg
     6421 gcgcatccca cactttcggc gtgaggtggc tggagtccac tggcgctggt ggtgtgactc
     6481 ctttcatcgc gttcatcatt ggatgaatgg attgcgtcat caggaacagc gtcaatacag
     6541 ggataagagc atagagtgct gatgctactg ataaataaga gctgtatgtt tcggcaagcc
     6601 ctggcaaacc catccatgtc accgcgttac tctgactatt caatgtgctc catgtatcaa
     6661 gatctgcggt cgccactttc ttcacgtagg cattcaccat aaccgcagta ataggccaca
     6721 tatttacgaa aagaataagc tggagatatt ttgcagcagc ggccacacca tttcccccga
     6781 gagccaaaag catgagcaat gcaaatgggg caaccatgta cgcaaacatc tcaagaaacg
     6841 cgatggctgc accggacaat tgcagccaga gctgcccctg agatgccatg gtattcgtac
     6901 gctttaatga tgcctcaaac aactgcatat ccgaggccag cccgagtggg gttttgtact
     6961 tgctggcacc attacggagt tcattcatga tgaacaaact aagcgtcgcg tcataagcac
     7021 caatagcttt gccgtacatg ccattcgccg acgccattac atccgtgaag ctcgctcctg
     7081 atgctgcacc tcccgcctca tctggagcaa ggataccgtt tgtttgccca atcaatgcaa
     7141 tcgtctgccg agcctctggg gtgctcgtta cctgtttaac ggtcgaccag acctcttcac
     7201 aagtagcaga ctttgagcta ttcacaccga caatgttggc agaggcgttc ttcagcgaac
     7261 ctttcatcgc ctgattaatg cgttccatgc tatccacgat atttgcagag aaaatatcgt
     7321 taaaaatggt ttctttccca gcaatttttg cgcttgagtt ttgggttccc ttgagacagt
     7381 tataggccag tgattgtact gtcgcgcaca cattcatcgc acctaacccg ctggctggtt
     7441 cgggaaacgc agaacaatag ccctggctgt caccacccca ttgtaagaat ttaacgaaac
     7501 gaatcatcgg accaagagta atgtcatcat caagcgtcgt tgcggcaaag tcgatcggcg
     7561 ccagtggatc aaatgccgtt ttgtagtctt tgagtaagcc ctggctaagg ttcgtggtga
     7621 cagttcccat cgccgcaata aaaataggaa tgccatcgac atttctcact tcaccagact
     7681 taactgattc aatcgtcaca tccacacgaa caattgccat cgaaaataga atcagcccaa
     7741 gaatccatga aaatatcggc acttctgctt ttgtcgggtt caacagccaa agccacgttt
     7801 tataaaacag gctggctcca gccagtaccg ctgccgtcgt tagaaatgct tttacaccgg
     7861 tgtattgtga gaaaatcagg gcaatcccgt tcagggcgga ccacacaaat tcaatatcgc
     7921 caactgtata gatgttgtaa tccatatcag acctcagtta cctgtgccac gaagactttc
     7981 catgaacgct tttctcttta atcggcgatc atcccatgca gaaatgacgt cagagctgtt
     8041 gcccacctgg ctacgggtta gctcataggc agcttttacc tgattggtca ggttttcaga
     8101 taacctagtc aggtcctgcc gccgcccgga aagagattcg ctgggtatat tcatagcgct
     8161 gatctttgct tcaacttgcc gaagcatgtt aataacaagg ctggtggccg ctatcgcact
     8221 gatatcctgg atataggcgt agccttcagg gacatcaaac gtctgaagtg aatcaagaat
     8281 agttgggatg ccaatataag aaataatgcg taattcatca tcagagatcc tgacatcact
     8341 aattgccttt gtctgaacct gaataaggag gtccgagatt gtatccttca aacctttgaa
     8401 accaccatcc gtcacttcag acataaccag gcattgcgac tttctagctg aagctggatc
     8461 gggggcaggc gaacatttga gcatttttat ggaaccacca gccggtgggc ccatgatgaa
     8521 atccgatacc gtcatcgtcg acgggcgaac ttccatcccc gctttttcac ctttagcgtc
     8581 ccagttgata attacagtac cgacaagcga catcagctgc tcagcaagtc tgtagccaga
     8641 tagctcagtc acgccaccga tattcattga ccctttatcg atgtccataa atgacatata
     8701 gaaaaggttt ccactgaatt tttcagcaaa ctcttcaggg tcttttgctt ttacctggga
     8761 agtcacggtt tccggcgact ttgtcattga tgaaccaaaa tcaggcaaca gtccattaag
     8821 agtgccaagc attgaagcag ggccggaact gacagcattc gcaaactggc taggtggacc
     8881 aacattttca gtaagaaacg aataagtagc gttgcatgaa tctttggcaa atttattcag
     8941 cgcctgcaat ttattctgga tatcgttaat agtcgcagca cagtcagcac agattgccga
     9001 taccgcgaca ttgaatgcat aaattgcagc cccctgtgca ataccgcgag caacctgaac
     9061 cagctgatct ccgttaatca tactgaagga accaaggaaa acatcgattc cgttgcagcc
     9121 tacggaagct ttgggaaaag acattgacac taaattggta ttcacgtttg tcgtgcggta
     9181 ggaaaatgag ccgccaacca ctccggtacg ggtggccgtt tcaaaactag ctgggctcgt
     9241 agtcgtcatc atgccattga aaatattacg catggcgtta tcggcaaagg catttggcgc
     9301 aatcatcgcc gtgcaaacgg ctaacgataa aagtaacttt tttgcacaaa catggaaatc
     9361 agcgcgcata tgcacctcct aaacttccac ccacattcag atagttcact gggtccgccg
     9421 ttggcatatc gtacccttcc agtttcttat ccattattcg ttgcaataag aacggatcag
     9481 agtccatttc tgatttattc acagtaatta cgccatcctc gccaatggta tattggcgct
     9541 taatatctaa agtggactgg aaagaggcat catcaatcag cttcatgcct tttgcagcca
     9601 gaataatagt attcttgagc tcctcagcag tgatcatgcc ttcgcttatt cgttgcgctg
     9661 atgacccatc ttttgagaca aggaaaatag ttggaacttc acggatttta aattgatcaa
     9721 taatctgcgc attaggtaca gagaaatcct ggaacaaccc attctgtagt ggtctgccgt
     9781 ccatgctgac aggaagaatg tcgacagaat agtaattctc cataaactgg agtatttgac
     9841 tttcttcgtg gcagaactga caagtactct ggaaaaagaa aaacagcccg gatttagtga
     9901 aaatatcttt cataaccgac tgctgatttt tctcgaccac cgtacgatgt gcatcgagcg
     9961 ctaccttttc aaccggttgc ctgcgttttt cagacatcat tggattttta aggaaataat
    10021 ctttcgattt atccgaaaac cgcgtaccga tatccagcat taaacgttgt gctgtataaa
    10081 atctcgataa attttcaggg gtcggattat ccatagcctt agtcaaaagc tgaggcatat
    10141 tgtcctttag ccactttgaa ttaagctcaa tcttttcttc ttgttctggc tgggtcgaag
    10201 atgcaacagg aggcttggtc tctgtaattt cttcctcagt tttccttgtc gggtcgtcat
    10261 accagaaaaa gcctttcttg aaggcctgtc ctgatacaaa gggagtgtct ttgataaggg
    10321 cctgctcttc atttgcatgg accccagtaa taaacaaaga cagcagcact aacatctttg
    10381 aaggtattaa cgttaatcga gtcatttacc gatctcctca tggaatagag gtatgatggc
    10441 atagaaaaaa acttcatgag ctcggtaacg gggtagattt caaacgttaa aaagtcataa
    10501 atgtgactac tagaaagacc ctgaaagggc tgttgaactt ctgtgtgcag ggaaggtatg
    10561 ggagctaaaa aggtgggaaa atagttatca gcatagttaa ttgaatctga attcaattcc
    10621 agacgatact tttaaaaatt tctagatgag cgccgcattc gattgatttt cgaatcggga
    10681 attttcgata aattaaaaaa actgaggata tattatttgg caagaaaacg gtttaatgtg
    10741 tttatatcat atgcagtatt gacctaaaaa atgccatttc atgttagtca tacttatgca
    10801 gagaaaagtt aggcagaatt gactgggtaa tgagcaacta cggttgaaaa taatattagc
    10861 ataatcgttt cagttacgtt aggccggaag ctgtctgaag aaaacagcaa catgtagtct
    10921 attttgcagt gaagtaagta aagaaatttt gtagttaacg aagaaacatc cctgggaatt
    10981 ttgacctcta cttaaatcac tcacataaaa taacagctaa tctaactctt cctgggaata
    11041 ccgtgctatt gccgatgacg tggacgacaa tctgcatcga ggacaatgag tatcattggt
    11101 atacgggaca atataagggc gcttacattt cgcacaagtt tcaagctcct cgatttcttt
    11161 cattgacgtt agaatctcaa tgattttatt tacctcagga gcgcagacga aatatccaag
    11221 gttatgcagc acactcatga tgcggttgaa gtcaatattt cgaaaactgg attccgggtg
    11281 aataggatca aaaccgaaat agtttacagc gacatcaata accgcagaaa aagccctgcg
    11341 gttatccgtg cggcgactcc acccacgaat ccctttttta aagttttgtt gaggttcaga
    11401 cctgaagtct atattttcgg caacatgatt tccatagagt atccttatgg agtttttatt
    11461 ggcaccaaga gacatcgttt ttgtagcgct caataacatg ttagctttag tttgatcgac
    11521 tttagccata ttcaataatc cttcaagagc actttatttg ccaaagtatt gaacacatcc
    11581 tgacggtggc atgtaaacat agtgtcgtaa cgcagtaatt ccattaaatc gtcactaact
    11641 ttacccaaac gaatatcggc agatatttcg tatgcccaag caaacacctt tgattttagc
    11701 gtggcactat catcccccag acctggcgta aagggaattg ttcgtggaaa gagaacgatg
    11761 tcattaatta atttaagtga tgacatcccg acatctttta ttgcttcaac ggtggcaggt
    11821 tttaatccaa aaaagatact aacaaactca ggtgcaagca ttgacataaa gaaaacttgc
    11881 attaagtaat cattttgaat gtctacacac ttagaacgta gttgctggat ctttctcgga
    11941 ttataaaatt cctcctgccc gctgagcaaa gttacttttt caccttcatt gtaaatgccg
    12001 aagtcagtat cttcactgtc atattctcga atgacgttag tgtcgccatc aactacagct
    12061 acaaagaatt tgtcccaaga gatctcttta cttaaagaac gaggaacgag gaagcttcct
    12121 ggccgagttt caacttcaaa tgcattatca atcatagctc caaatccatt accaaatagt
    12181 cggcgttgga ggagtggtga tccagtttga tacttagaag atccatgaag aggcaataac
    12241 attaagaagg cacggtaact acgaataatc gaaatctcgg tacgtaatag tttaccgagt
    12301 aaattacgcg tgatgttatc tgattctgac atgtgcggat tgtccttgcg ccaatacaat
    12361 tgtacgaatc ttctacatga aattaaggtc tggattctga aggatgaaaa gtaacttttg
    12421 gaattttaat actgccgctt tgaaccttaa aactaacctt tcagccatta cccttacttg
    12481 taacactttt taaccaaact aaccagaatt ttatcaaaaa accctggcct tgctaacgaa
    12541 atgtgaaccg tataaaaaaa cctcaaagcc tgagtcgtta tcggcattaa gtagcaataa
    12601 ccttagggaa aaatgcgcgt aagcatgatt attttttcga ttgtttaaca tgactactac
    12661 atagggtcac tttttgctga aaagtgaccc tatgcaccat cgacataggg tcacttcttg
    12721 atgaaaagtg accctatgta atcacgacat agggtcactt tttttaaaag tggcactatg
    12781 cgtcaaaatc atagggtcac tttcctttaa aaagtggcac tagatagatg tataacttag
    12841 taggtaaaac agttcttttc agatcacgat ctcagcaata agcattgctg gaaaagttac
    12901 ttttcttcca cgataaaatg cggacctttt ggccgttccc gtttacatct gatgtcactc
    12961 actctcagtg cttcctccat tcttaaattt gccatgcaaa attctcgaaa aaattcacag
    13021 aaaaaagtcc cgttcgtgaa aaaaaattga aaatcacttc ggtatagtga tttcacatca
    13081 ggaggcccca tgtctaaatc gaagctatta aagtcattgg agatttcacg tactttattg
    13141 cgctcacaaa tcgatgttct tcgagaagag gctgaagaac tgatcaccaa ttattggatc
    13201 gagtggaaag aacggaatca cattgagagg aatttaccca gggatagcaa aactctaaag
    13261 aaggagtatc tgggttcata tgcaccaaaa gttgagctaa ttggtaatgc caggaaagtc
    13321 accattacct ggcatcagtt tagcccgtat aaaaacaggc cacctagtca catgtcgaaa
    13381 cggatttcac cgatgaaaag tggaaaatat acgaaaagct gctttgttaa tcatgccagc
    13441 tgggaatatg aaatgattga aaaaacggag ggattactgg aaccctacag agaattactt
    13501 gagttctacc atgctgcata tatcgaactg ggacgaaaag tccgtcaata ctcaaaatca
    13561 gaggtggcac aatgaccgat gtaacaatga atgaaagcga gcataaggta cctgacgtaa
    13621 atacccgctc ccctcatgcg tccgataata gccgtagggt cgtgcaaagt aaaaatgaac
    13681 attacggcat ggctacagta actattaccc caggcacgcc agactttaac cgatttctca
    13741 cggcacgtaa tcgttccgta ataagagggt ttgatgatgt cagtattgcc atttcatcct
    13801 tattcagaac ggtcaatgca gttaagcacc ccgaactagt gcaggcaatt caagactggt
    13861 tcaatgaatt gcacgaagaa aataatctaa tgaagaacaa tctggagcgc cacattgcca
    13921 ccatccatat cgatgaaagc gatccgttct tctcatcaac tgagttttca ccgttcagat
    13981 ttgaacctgt acagttaaac ttcaacaatc aaaacacgat gcgcttttat aagcacatct
    14041 ttgaaatgaa caaccttctg acacagatgc acaaattcaa ctctctgggt caattaccgg
    14101 ttagcgatta caacgttatg gcccacaata ttatccggag ccttaatatg tatatcgaac
    14161 gggtcaagaa aacacttaac gtttcccgcc gcgcaaaagg tgcttacagt cctgatgagt
    14221 tcatcgaaaa ggtaaagcag tataaaagcg tgcaagcata tattgctgcg gaactgtctg
    14281 gtaaacgccg gtaaggatta tttgatgaat ttcagggcac tcttcttaag tatgcaacga
    14341 gtttttggca ttttctccag aagggagaat gatgtatctg agttaatgat gaaggatgca
    14401 gcgaacttct cgccatttgc tcagattata ggagagcaga aatacactgt gccggaccac
    14461 ccgaatcctg aagtcctgaa attcattgaa tacccaacca ggcctgcggg gatacagaca
    14521 tttaatgagc agtctattct ttcgttgtac agagacaagc tgcactctat ttctatgatg
    14581 ctggcaataa gtgatggaga cattcgggag gatgcctata ccttcactaa tctggtgtta
    14641 aaaccattga ttgaatacat ccgctggatt catttacttc ctgcgtcaga gaatcatcac
    14701 cataatggta ttggaggatt gttgtctcac agcctggaag tggcgatgat ctctttaaaa
    14761 aatgccaatc actctgaatt acgtccgatt ggctatcagg atgaagaagt ggttcgccga
    14821 aaagtttatc tgtatgccgc ttttatctgc ggcctagtac atgatgcagg gaaggtatat
    14881 gaccttgata tagttagtct caacttgagt gaaacattaa catgggcgcc aagctcgcaa
    14941 agccttcttg actgggcacg agaaaacaat gttgttgaat atgaaataca ttggcgaaaa
    15001 cgtattcata accaacataa tatatggtcc agcgtatttc ttgagaggat tttagatcct
    15061 gtctgtatgt cattccttga ccgagtcaaa aaggagcgtg tctatgcaaa aatggttacg
    15121 gcacttaatg tctataacga cggcaatgac tttttatcaa aatgtgtaag aacttctgat
    15181 tattactcta caggtactga tcttaatgtg ttgagggacc cgataatggg tcttcgttcg
    15241 aatgatgctg cggctagggc aataggtacc attaagcata actttactag cattaatatt
    15301 aataactata aaactaaacc catgcacatc attattgtaa acggtgaagt ttacctcaat
    15361 gaaaacgcct ttctcgactt tgtactgagt gattttgcag cccataagtt caacttcccg
    15421 cagggagatg caggcaaaac agtacttgtc gaatctctgg tgctacgggg ttatgttgaa
    15481 ccttacgacg atgaacgtgt ggttcattac tttattcccg ggacatattc ggaaaatgaa
    15541 attgcaagca tcttccggaa cggtattggt aagctggaat tttataacct cttaaaactg
    15601 cgttggatcg ggttaatttt tgattcatat aaaatcccgg attcagttcc tggtctgttc
    15661 agcgttaatg cgaataaaga tttcatctat attgatgagc agaaaactgt aacggaatac
    15721 agaaggccgg tcccgggtcg tgagtctgtt accagagtaa cggacactgt caatgacgcc
    15781 attgagaata ccccacagta tggacttcag ctcgtaaacg gccctgatgc cgacagtaac
    15841 aatattatta gcagtgaaaa tactgaatcc attacggata gtttggaaga gtctggcgcg
    15901 gatatctcaa acgagatttt tgaaacgcaa gttgttacgg caattgatac tgccgaaacg
    15961 gtaaacgcgg atgagccaga acaggtagaa gagcatgacg accggagcca gattcatctg
    16021 gttgaacaac ttcatgaaat gctgttgagc gccccactgc cccatcatgc cgtcattaat
    16081 attgattccg taccttacct ggatctcgat gctgcgattg cgttaatccc gggtatagat
    16141 gaggcggcat tttgtaatgg gccatttttc cagttgacat accgtgatgg gtctctagat
    16201 ggtatgtgga tagtccgtga tgtaaacaat ctacgcctga tacagctggg ggataactgc
    16261 gccggtatgc aagtaagcac atcggaacca cgaaacacgt catcgctgaa atcactattt
    16321 gatacgtcaa tgtaccaacc gttggatatc cctgaagctc catcagtaaa cgaagctgct
    16381 tcgcctccac aaacaccgct tgaactcccc caaccacgac tgaatgcacc tgtggctgaa
    16441 gaggccagct cggtagctga acagactaac gcccactccg aaccggattc cgtcatcgct
    16501 actgagtatg agcaatatgg tcatttgctg gaagagaccc ttgatagcga tggtgaggca
    16561 tattctgatt taattgcctc tgacagtact gaggcagaat accccgccac tgatccgcag
    16621 agcagcgatt ttgcccaatt acctcgtgaa acggccctgt ccgttgcgcc cggtgatttg
    16681 gattactcag agggtgccat taagccacca gcacccgatg caaccggaaa agaaacaata
    16741 ctaacaagtc ctgaaccagc agaggatgtc cgagaaacgg tagccgccgt ggagaaagcg
    16801 agtcatcttt ctccggcatt ggctcgactc tttgctgtaa gcacccatgc tgagaagaag
    16861 catgaaaaaa cgcaggaacc ctcccctgtt aaagaggtta aaaatccaac ctcctctacc
    16921 acagttaagg ctcctatttc cattgaacca ccaggcgctg aagagaaaga ggcggtggaa
    16981 gaattcaccc tgttaaacga cggtgaagtc acagaactcg aatatgtcga gattgccacc
    17041 atgctccacc aaattctgac gaagttatct gggtcgttca aaagaaagag gaaaaaccgg
    17101 ttcatggttt tgacccagaa caccttttat ctgacgcagt catgtattga aaagtacgga
    17161 acgcagctaa atgcgcctga gctttttaac caacttcctc agtaccaggt cacatccggt
    17221 gccgtagtca atacaaaatg cattgcattc aacataccaa ctctggtcgc cgcatcagac
    17281 cgggcaaagg tcgatattga gttaattatt aacaagctaa aagaggtcgg gaatctgtaa
    17341 tgacaaaatc aaaaagaacc aacctgcacg ctcaggaaaa cttttacagg ccgatactgg
    17401 agtacagaag tgcctcagtg cttttaattt gcgccgtcat aatgctggtt atgggctttc
    17461 gttcagatgg ggtgaatatt gcccctatca ttctttatac cgctgttttc ctgcttttgg
    17521 tttgtttgta ccgttgtaaa acggcacacc cctatttgat ggcacactgg cgcgtatttc
    17581 agcgccagat aatgtttatt agcctcaagt cgctcaggac gatcaataaa agtaactttt
    17641 tctcgaacga gcgaaagtat cgccagctgg tacaagagta taaaaaaaat aaccgaccag
    17701 taccggatag aaaaacctac ttttgtaacg gctttgaatg gggtcccgag catgcagacc
    17761 gcgcatatca aattgctaat ctctcaagtg acaaaagaga gatagccctg ccatttgttc
    17821 taagccctat agcgcgacat tttgaaacaa tggccagatc catggggggc aataacgcca
    17881 tctttgctgt tgaccggcgg gcacccattt ttgtgaccga agataactgg ttcggacata
    17941 ccctcattac cggaaacgtc ggtaccggta aaacggtact tcagcgctta ctgagcatca
    18001 gcatgctgca tttgggccat gtcattgtgg ttattgaccc gaagaatgac gctgagtggc
    18061 gacagtcact gatggatgaa gcgtcagaac tggggttgcc attttataaa tttcacccag
    18121 cccagccgtc ttcatcggtg tgtattgatg tatgcaatgc ttacacaaat gtttcggacc
    18181 tcacctcacg acttcttagt ctggtttcgg ttcccgggga agttaaccca ttcgtgcaat
    18241 atgccgaagc tctcgtctca acggtgatca ctggtctgtc ttacacggat aaaaaaccgt
    18301 cgatatatct catccacaaa aacatgaaaa gccatatgtc tgttgtgaac ctaacgataa
    18361 aagtaatgga atgctgcttt gcacgacact acgggcctga tgtctggatg gaaaaggtga
    18421 aatacgcttc gaatgacact cttcaggttc gatttaagcg cctgacagaa tggtttaatg
    18481 cccattttct taattatgaa ggcgccgagc ccattgaatg gattgatacc gtcggtcggt
    18541 tagtggatta ctccatgtct gatcctgaac acatgtctaa aatgacggcg ggtattatgc
    18601 cgctatttag tcgactgact gaacaaccac tgaatgaatt gctttcgccg tctcctaata
    18661 cactcacttc gcgtgaaata gtcacttcag atgggatgtt cagtaccggc ggagttctgt
    18721 atatctcgct cgatggcctt tccaacccgg aatcagcccg ggccatttcg cagttgataa
    18781 tgtcagatct tacgtcttgt gcgggtagtc gttataacgc tgacgatggt gatatgtctt
    18841 cccattcaag aatcagtatt ttcgtcgacg aagcacactc tgccataaac aattcaatga
    18901 taaacttact cgcacagggt cgtgccgctc aaattgcatt atttatctgc acgcaaacta
    18961 tttccgactt tattgcagca gcgaacgctg agacggcaaa ccgtattaca ggtctatgta
    19021 acaactacat tagcctgcgg gtaaacgaca caccaacaca aacgctggtc gtagaaaact
    19081 ttggtaagag tccaattagt acaaatatgg tcacgtatac gacaggatct gaaaccactt
    19141 tgccgcataa taacttctct gggtcaatat cagaaagaaa gcaaacaacg ctggaagaaa
    19201 gtattccaaa agagcttttg ggccaggtcc ccaaattcca tattgtggcc agacttcagg
    19261 atggtcgtaa agttgtcggg cagatcccga ttgccgtaag tgaaaaagcg atgaaaccca
    19321 atacgacgtt actggagatg ttcctgaaac ccgccggaaa agtaactttg cgccagaatg
    19381 tgggtttaag ctatttaaat aaatatctaa ggaaattgca ttgatggatg agcaaattac
    19441 tctgacacaa aatcaaattt tcagtgcatc tctgaaagta agtaaaagcc gctcgctcgt
    19501 taagcggcgt atgcaatcat tagggttgaa gtttaccgaa tcgcaggatg tccgtaaccg
    19561 actggcgggg atagaaaaag gtgctttgaa atgtgtgggg caattttgcc atgacaacga
    19621 tgctgagtct ttatcagcga tggcgatcat cctttccgag ctttttctgc tgcaaggcga
    19681 actgtccacc agcaatgaat acggtgacca tgaaacgagt tactggaccg tagcacaagg
    19741 cccatgtgat gaatgggtcc agtctctgct ggcgagcgaa aatggtcgac gtacatttaa
    19801 tagctttcgt ataactttcg acaactccga agagcgccgc agcctcgtcg aaaaaaatgc
    19861 caaaatgctg gggagttatt tattgcctta ttttgtcaat tttacaaatg ctgcatctgc
    19921 gtttattacg ctgcctaata gcatcacgtt taaacaggtg caacgcaaca agccgctcat
    19981 tcaccctgaa accacattaa gtcacattct cacaatagaa gattcggcgt ttctctctcg
    20041 gatcaaattt aaacttatct cggcaattga ccgtctgccc gacccgtccg gtcagtatgc
    20101 caacatgttc aatcacatca tggatcgggc gctgctgacg cacctcaacc gggagcaaat
    20161 tgatagccct tgtgtgtgca aaaaggtcat ttccacctat gcagacacta tgctgacgct
    20221 cccgatattt aatacgacga ttaccgggaa ataccggcac tggaccccgt gggggattaa
    20281 ttttgtcgag tttagtcgtc aggctgcaaa ggaaaaatct tgtgtctatg ttcctgaacc
    20341 cggccaaatt cactggaaaa gtccggagca taaggaactg gcagagtaca gcctgatcaa
    20401 ccagataatc ccgcagcaat accactggtt gctgggtgtg ccgacaatct ggcgcagtca
    20461 ctatcgtgac catagcaaac gattagactt attcaaggag tggagggacg caaatggctg
    20521 cggataattc tgctcgtgca atattcatca ggggactgct tttactaggg atgatgcttt
    20581 atcttattta tagtctgggg ttccagaaca ccgacgagct aaagcaacaa atcactcagg
    20641 aggtgaacgc cagtcgtagc cttatttcta atgaccggtg gttatctgtt attgcaaata
    20701 gtgaggctac tttatccacc ctgattaatg actataagct gatcgactat ctgaatacga
    20761 tactgatccc ggacacatcg acacctgcac gtggaatgaa ttacgttgcc gaaaagatga
    20821 catcagtaaa ctacactgtg gcaaaaaatc tacccctgct gatttatcag tccattttcc
    20881 gctggaatct gatactcggt tggctgattt tgttttcacc atacctaatt gctatgctcg
    20941 ctgatggcat gtatcagtgg aaattaaaac gttacgtttt cggtaatgta accgttcagt
    21001 tttacagaat ctggttcagg gccttctgga taattggtgc attaaccttt atatacctat
    21061 caatgcctaa tatgtcactt tttaacaata tcgctcaact gttcccccct gtcgcattgt
    21121 tgatattggg aattgcactc aaccgtttgt ggtctaactt tcaaaaactt atgtaataag
    21181 gtggtcagat gtttaaacgt aataataaga agtcacctgc caatatcaaa gaatcgcttg
    21241 atgagaacgc tgaccgtttc tataaaatgt tccgaatcca taccacgtca aaagtggtta
    21301 tttcactgct ggcattagcc gccgcagggg tttcggcctt taatatttac gataagtatc
    21361 aggaatccga aggacgaatg gatcatatcg ccgtcgtgcg tatctcggga gcaatgggta
    21421 ccggttctga ggtcggcgat ggatctgtga tagccacagc actggcgaaa gcgtacaaca
    21481 acccacatgc caaagccgtc attattgaag ctgagtcagg tggcggcggt ccttcagacg
    21541 cgattattat ttaccgccag attaatgcac tacgaagcca ccagcagaag attgaacggg
    21601 tttcacatgc cgaatccaca agcaagcccc gtaacacgag tgatcacagt gcttcacgtt
    21661 cgcaggccga tagcaatgcc gaactgtcga aacgtaatac gctggacgtt ctctccgccg
    21721 ggagtgggca tttcatttct gatatggaaa acagctacaa gcctattatc gtcagcgtca
    21781 aaggaatttg tgcgtcagca tgctactacg ctgtgtcccc tgctgacgcc atttatgcag
    21841 acagtagcgc tctgaccggt tcgatcggtg tccgaatgga tcactggaac ctgtctcgcg
    21901 ttatggatac tgtcggcgtt aaaaatgagc cactcaccgc cggggagttt aaagatgcac
    21961 tcgaccccta tcatccactt tctgatgcca cccgcgattt tatgcagaag caaatcctgg
    22021 atgcaatgca cgaacaattc attgcggacg ttgagcaagg caggggtaag aaattattaa
    22081 gccgtccgga ggctgatgct gtcgcactgt attccggcag ggtatggaca acgcagcagg
    22141 cgatccggta cggactcatc gacggtgacc tgacacctgt cgaagtccgt tcacgcctgt
    22201 cccaaatgta ctcaaccact atcttcaaga cgtacaacga gccacaacgg tcgcttcgct
    22261 ccgcgctcgg gatgttaatg agtctgtcga ctaacgtcga aacgctggca ggaacaacct
    22321 ctcggatact tgattctgtt caggccacca gctatccgac ggtgagataa gatggatgca
    22381 attttgatgg tgtggcgagt gataggaatc agcattattg tcctagcact cattgtgctt
    22441 tactgcctga tgggatttgt atcgtcggcc attgctgaac gcaggacagc cagacagatt
    22501 gaaatatacc tgcctgatga tgcagaaggg cttctgagcg acttaacgct gccggtcaat
    22561 gccaccggca ccacgcagat tgatcacgtt cttatcgcct ctcatggact ctacgtcatc
    22621 gagcagaaga actatgcagg aaagctctat gggaagctgg aggagagcca ctggcgaaaa
    22681 tggaaatcgt cagggacact aaagttacaa aacccgttca ggcagaacta cgggcacata
    22741 agggccattc agtccacgtt acgcgccagc gagctggagt gtatcaatgt tgtcatcatt
    22801 aacggcccct gcaagtttga aggtgagaag ccagactggc tttgcatggg tatggaggag
    22861 ttcgttcgga aagtcactga gaggcgccag ttaaatgtat tcaagcctga agcagtaagt
    22921 tttatccgtg gagagctgaa gctaaagaga aagcctccgg gcctttatac tgatttgaac
    22981 catatccaca acgttaccac tcgatacaaa acaaccatgc ggctggagca gcgtattacc
    23041 tataaccttt tgcgcttttc tcggcttata ctgagtaaaa tgttcagatc ggcaaagtaa
    23101 aaaaaaccct gcaaatgcgg ggttttttat ggattcaact ccggaatatt ttgccacgct
    23161 tcctgcataa ttcgcatggc ctccccaatc gatagagacg gccataaagc ggcgtcagtc
    23221 atttttacgc caaccggctc acattcgata gggacaagaa ttaaaacaac gcgagtccct
    23281 tttatcttat acccgagatt gactaccggc tgtctgtagg tagccagaac tcctgcaccg
    23341 tactccgcag gtttgctggc ccagctccgc accagagcgt aagcctgcgg catatagcga
    23401 ttatccagat ctccctgcgg ccgacgaaaa gggacgatgt catcggaacg gtaagtagtg
    23461 gtggttacgt ttgccgttgt cattaatcaa ctcctcgcat ctgactttat tgaatctgaa
    23521 agtaaagggt ttcacgatca gttcccaatc gtggacactg atagtgtggc tagaattttt
    23581 gctccatttg ttgagcttca aaatctcttg cagccttcaa gagtgacccg ccactgtagt
    23641 actccagttc agaaatatca tcctctttat tcaaccattg tggggcggct tcattgccgg
    23701 tcttacctga acccacatcg ctattatggc ttcgaggcat cactacggct tccacttctt
    23761 gataaacgcc attgcgtttc actaggatat taactgtttt cggtttgctg tacttttcgc
    23821 ggactttatt aaccagatat agaacgataa tccagcccac cagcacgtaa agaaaagtat
    23881 tcatatgccc tcctttgtat gagtctacta taccaggttc cgttccagcc caaataggtt
    23941 cagtgcatga aaaataggct ttcccgtccg aatctgcaaa tccactctgg cgtgcctttg
    24001 ctgccctgcg tgagattttc tttccggttg cgtatgacag attccgtaaa gttactttct
    24061 atccgtcttg attaaaacca gatcctctga cttcaccatc caccagccag aaccacggac
    24121 gcaaaaccat tacgaaaaaa tccgtcaact ttaccccctg tcgatagccc cccgacaatc
    24181 gggtgcctgc accctgtatc aatcaccacc gcccccacat ccgccaccag cgtccccact
    24241 gtcgccccca acgccagtgt ctgagtagcc ggagattaag aagaaaccac tttcgcctgt
    24301 gttcgctcca ctcttactgg actgcgtaaa ctgacgccct ccgccctgga aacggtaaag
    24361 tgccttttta ttacggagac agaggtaaag acaaacagca acatagagga ttaatgcaat
    24421 attgagtaac atcacaaaac tcctgtgcgt catcgttaat ggactcggta tttatattaa
    24481 agtcccttgt tcttaaattt tgttaacacg ccccttttct cccgaccgca aattattatg
    24541 gagcgagtcc gtcacagacg acaccgcagt cgtcagccag acatatccac cacgcaggcc
    24601 atgaggtacc gaagatttct ttccggatgc cagatctctt tttagttccc agcatcatca
    24661 ccgcccccgg ctaaaccatc tgcatgaggc caggcttaca ccaccagctg cgggccccgg
    24721 acagcccaat gatccttttt ccgtatgcca gctctgagtg ctgagcccac aaaaaagaaa
    24781 cccggtgtta ccgggttctt gtgctaatca actggccttc agcccatggc tttgcaggta
    24841 gctattgagc gcatcattgg caccggattc ataattccag tttcgccaga ccagctggct
    24901 gtcgtggtcg cgatgatcac ggatgacaat ctggaaatgg cctgctggct ggaggttttc
    24961 gataaagacg ttctggtacc cagcggcgac agtgcgtgcc tgagccagtg ttaccggtga
    25021 ttcgctgctg aataaatcgc tcataagaat ttccttcata atcgccaggc cggagcctgg
    25081 ctaggggtgt caggccgcga ggtgtttggc gagtttaacg aagtggtctt ggttgttgta
    25141 caaaggcatc tgataggcca ggtccgaaat aaacatccgg atctggtcaa acgtcatgcc
    25201 gcagttgaag ccagtttcgc tgacattagc ccgtggaaaa tcatcgcaaa attcgtttaa
    25261 caccttgtca tggacaatgc cgatattaag gagtccggca ggaccgttat cacggaaaga
    25321 cacaacacgg aatgcgccat accagcccga ctggaaaaag aaggtaaacg cctgggttgc
    25381 aagtccgtta aagagctcgt aacgctggct ggcactcagt gactggctgt ccatgctgta
    25441 cacgcgattg ataaagacgc ccgggccgat gtttgcgggg atctgcccgt aagcaatgca
    25501 cggcatggcc atttcagatg cgtctttggt atcatcaatc actttatatt gcgcggcgat
    25561 aatatccgcc aacattgagc cgccaagtgc gctaattgct gcgatgtcat taacctgaat
    25621 atttaaagtt ttcatatttc acctcattgt ttttcttatt atttcatatt cagcctcgtg
    25681 gctaacaacc tgatgtgaaa tatttaataa attcatcagt aggacaaaca caaaccggta
    25741 gaccgttcaa aaatgtcctg caaatgaata ttgctggcat ccagattaat ctggacaagc
    25801 tctgcattat tacctaaaac agaaaacaaa taaaaatcaa acccgcctgt attatcatcc
    25861 ttaaaaatgc ggaaggaatt aatattaaca gcttcgtcta gttccacgtg aaccccataa
    25921 cgtacttccc cgtcatcaaa ctgtgcaatc cggcgcaccg atttgagacg gacaaatttc
    25981 gccagctcca gttcgggcgt tggggagaaa gtcagtgcct cgaagatcag atctgctgtt
    26041 tcatgttgct tcctgcggtc gaactcaaac aaaccgccac cattgttgcg atagaccggc
    26101 tgaagcgcat tgatcaagtt gaacgtctgc cggttcctgt catatttata catttatagg
    26161 tgctccagtc ctgccagtat ccaccagcag ggcataatgt gattagtgcc agttggcgat
    26221 cagctcttcg cattgcaaag aggagaaaca ctgctgggcc aataccggca gtacggcaat
    26281 gttgggtaca cggttgtgac accatgcgtt aaacagcatc tgccaggggc cagcgtagta
    26341 accgcccatt ttcatttttt atttcggagt atggcaggcg gaaatagcct gctgatagag
    26401 actttcattc tcggttccag agcgttgttg cagtgcagga taaataaagg a
//
LOCUS       DAMZII010000066        26347 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZII010000066 DAMZII010000000
VERSION     DAMZII010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.066"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            294..1220
                     /locus_tag="POA77_002627"
     CDS             294..1220
                     /locus_tag="POA77_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366030.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFEGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYVCPVQQIIDTPLIGYEYVLIQRILARELKFPSPIVGARKTNHGIIVRFSEELFQ
                     IFETMSWKERVEKQISRLPKNTALDVIKKLTEVTTIKYNHNGCFPLYTLPPDACFVIR
                     HTEVERLINLYKKRESHPISPSRMTTPLSRLFWLACKHNDIISPLLNHPYKLLSIFEQ
                     WASGDGIGEKLDAETLKNALKRGSPSSTSLSG"
     gene            1360..1545
                     /locus_tag="POA77_002628"
     CDS             1360..1545
                     /locus_tag="POA77_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6366031.1"
                     /translation="MTSYQLLRLRQVEQKTGLKRSQIYLYMKEGAFPRSIKIGPASVA
                     WLESEIDEWINKKLSDR"
     gene            1567..1749
                     /locus_tag="POA77_002629"
     CDS             1567..1749
                     /locus_tag="POA77_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366032.1"
                     /translation="MNINIPALINFDPAGIKKSTCMYISDLPGIVLYDTIPFTEYSAE
                     LLSTYSVKESISCFHP"
     gene            1734..2315
                     /locus_tag="POA77_002630"
     CDS             1734..2315
                     /locus_tag="POA77_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDI6366033.1"
                     /translation="MLPSLNHIILTVALQALREGNIHHCETMGFTYDEMNLLGCLSIN
                     DLITLSQAPLPLVDITIRHDVLQKLLASSHEENRRQEQLNRAVRLGGSIALMNRYFGV
                     GSRETCARRRLLGVSVPNGRTPIPDEETDAAIWHQWQKYRVENIDSPEALDAMMLVTE
                     NLSSCTAPSLTVVWNCITHCERKNTVRRIQRAR"
     gene            2305..2514
                     /locus_tag="POA77_002631"
     CDS             2305..2514
                     /locus_tag="POA77_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI6366034.1"
                     /translation="MPDEIDRDQAFNERCLEALIEQSRLRPTPTPSLQHCRLCGKAIP
                     EKRRQTLPGVTTCTDCQSILEKRRR"
     gene            2718..2885
                     /locus_tag="POA77_002632"
     CDS             2718..2885
                     /locus_tag="POA77_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366035.1"
                     /translation="MNDGSLLEKVVGHMEEKLTGNELEMLYAAADYRRAEIVMNQYFD
                     KVPGNVWKYVR"
     gene            3454..3687
                     /locus_tag="POA77_002633"
     CDS             3454..3687
                     /locus_tag="POA77_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366036.1"
                     /translation="MRKPVYYAYVAQESHPDSQGEKNTWWTKVGVAFPHHGKSGLNIV
                     LTPGIAVSGKLVLLEPREENNPPQNTGPIETVS"
     gene            3760..4470
                     /locus_tag="POA77_002634"
     CDS             3760..4470
                     /locus_tag="POA77_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6366037.1"
                     /translation="MLSTTAFLAIAMQCAASIHPSTAFDVARVESGFNPYAIAEIVPK
                     AGPSFGDQHIISHLPASRGDAEHILRRLSAQDRRYSVGLMQITSTNFRHYGVSATELL
                     DPCTNLSVFEHILRDCYRRGGTLKRALSCYYSGNFSTGQQPETAFSGTSYIQRIGYPP
                     GSSRYAVPGTREDKSSVAVPLKTAPASDSSRHHVFWPGVVVRGVPAKLRQEKIFIAPF
                     SAPSVRRSFSLSPREEEE"
     gene            4470..4763
                     /locus_tag="POA77_002635"
     CDS             4470..4763
                     /locus_tag="POA77_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDI6366038.1"
                     /translation="MILFRKLKAVVPPVSACTLTTFSATPALADGFQRANTVMQKVST
                     GLHGLAAITITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
     gene            4776..7514
                     /locus_tag="POA77_002636"
     CDS             4776..7514
                     /locus_tag="POA77_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HDI6366039.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLL
                     PVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDA
                     MKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLH
                     GLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTV
                     CYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGN
                     AVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGL
                     EVKEFAEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVS
                     QRLDLDVALDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAE
                     ISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYPGKRDKAPWGDAMALLRTP
                     SGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGREDSFS
                     PDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQ
                     LMKILCTRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQEN
                     GLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYR
                     ITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDD
                     IAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKR
                     GDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAKAL"
     gene            7532..8239
                     /locus_tag="POA77_002637"
     CDS             7532..8239
                     /locus_tag="POA77_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDI6366040.1"
                     /translation="MKTRFRTLILACVIASPLAHAGIPVAVDADPMRDVQWAQELKRW
                     METARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLSG
                     GSSGQFDALYEKYKIFDTCNTAQSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGE
                     INSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAERVK
                     QWNQQQLNAPTADLNNL"
     gene            8247..8489
                     /locus_tag="POA77_002638"
     CDS             8247..8489
                     /locus_tag="POA77_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HDI6366041.1"
                     /translation="MLSKKIILISTITLCFSIVGCKEEAKTTKWYRDHPDELKLAYEK
                     CQKSGDASDNCKNANEAHYQIKQLNAPTPDLNNLEE"
     gene            8493..9566
                     /locus_tag="POA77_002639"
     CDS             8493..9566
                     /locus_tag="POA77_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDI6366042.1"
                     /translation="MSGGLFVGVEKYLIGGITDATKRLMMSYSSMMMGLAAASATIYI
                     MWRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANLGGYLDGVIDAINGIKEGFSGSD
                     NIWQLLDTLWNKAKVLGKTLHDMDNSTYVKDEGMTAQFCVWLGIFALMILAGFVSMVA
                     EVMILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAGILTILFTALSLRIVVNYLNLV
                     LNKATKVADSSTIVELGAQVCLAGIFAAILVYISAKVAAALAGASATVSMQGLAAVGI
                     GAAAFGAGKLAGGAVKETKRSAQDNIQGWKDANAGKPGKPGGELGYNASKARKYAIEQ
                     MIARNEARRLAQSRESAVKKFNS"
     gene            9658..9795
                     /locus_tag="POA77_002640"
     CDS             9658..9795
                     /locus_tag="POA77_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366043.1"
                     /translation="MKRLIALALLCALSGCAQNTPPSAFSGKGEPVNTPQIMAELNSH
                     E"
     gene            9788..10471
                     /locus_tag="POA77_002641"
     CDS             9788..10471
                     /locus_tag="POA77_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDI6366044.1"
                     /translation="MSEQKLIAESRTFEQQMIERDKRATKAGFVVGGMGLLIAVLALV
                     AVVVMLPLKQTDVELYTLDNHTGRVEHVTRTSKTSLTATEAYQKAMAANYVKVRERYV
                     YPSLQDDYETVQVYNAPQVNDDYLALYAGKNAPDKVYKNGAHTVKVEILSNQITDATA
                     PDRVATIRYKKIIRRLADNSTRNEYWDARFTFHSNPDKEMSDAEREINYFGFTVTSWQ
                     TDREIRGGE"
     gene            10468..11376
                     /gene="virB9"
                     /locus_tag="POA77_002642"
     CDS             10468..11376
                     /gene="virB9"
                     /locus_tag="POA77_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HDI6366045.1"
                     /translation="MMKKLTFTALALMMCGAAWAAAIPQASRYDSRVQQVIYNPQNVT
                     VVNTKPGFMTTLVFDNDEAVISAKPGFDEAWEATPDANRVNVRPVALTQGAPGEDGNT
                     TQVVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQPAFQVSYRYPGEERDKASR
                     EATARQREWEQKQQQASVQKALNSAQTPRNWSYTKYPGKGSFNIVPDFAYDDGRFTFV
                     GFSPSKSIPSVTKELNGKEHVVNSSIQKKGDFTVLVIQEVTPRLVLRSGNAVVGLENS
                     GFGKVHAADGSTVSRQVERVEKPESN"
     gene            11412..12662
                     /locus_tag="POA77_002643"
     CDS             11412..12662
                     /locus_tag="POA77_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HDI6366046.1"
                     /translation="MNDENKMPVPDEAQAFPAHAEDDIATLEREARAKREAELLNAQD
                     DEEKDPVQPAVNKLKKRKRGKATAFLAIVAVTLIFLAWGGNWVYRNILWQPTEEKKQD
                     TAPQTNKTDYRQRNDLGMSTDTAEEEPEQQDNGQSSTGGTGQVAPAAPPELNKASFLI
                     RRDGSATTQNPVKTRQQEMTLTSATTTGQQDSSAATNTAAPPPGQSPAPLRRIPYNPD
                     LYIPENTSIPCSLDRRFVSDRAGKLRCTVTTDIWSASGNTKLIEKGTTASLLYRAIAE
                     EGMKHGQGRAFIIATKLRTRQPPYLDIPLVDTSAAGELGEAGVDGWIDSHFWERFGGA
                     LMVGMIPDIGAWASNSAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGE
                     IINLITGEDIDFSNIYTLRLKNDR"
     gene            12652..13677
                     /gene="virB11"
                     /locus_tag="POA77_002644"
     CDS             12652..13677
                     /gene="virB11"
                     /locus_tag="POA77_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HDI6366047.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELYTKINGVW
                     EQHAVPLSYEDCYNFARCLAKHHGDNIEDIKPGLSATLESGERCQVIVPPACERDTVS
                     ITIRKPSKVQIPHQSYIDAGFYNRVTGEEKTETHDEELIALYNTKNIPLFMEKCVEYG
                     KTLAVAGETSTGKTTYMKMLIGYIPVHLRISTIEDNPEITFFIHKNYVHLFYPSESSD
                     EKGSIVTPASLLRWNYRMNPDRILLTEVRGAEAWDFLKITGSGHEGSMTSIHAGSAKE
                     AIDGFITRCYENPQCAQLPYTFMLRKVLDSLDVIVSIDLDGNVRRMNDIYFRPIHRNQ
                     YFEEMKA"
     gene            13674..14072
                     /locus_tag="POA77_002645"
     CDS             13674..14072
                     /locus_tag="POA77_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HDI6366048.1"
                     /translation="MIKKLKVIMLAAIVTGCSSPPPPVPVEWDKAAAPLNTSLSQWSD
                     NNVIVPSPTVNGKWTSSISAQSFYDTIWTPAVFYAVAHSTRIVVTAQSGTDFFNAKNW
                     LRQNGAKGVIEYQPVFNCLTCRETTIYFSR"
     gene            14108..14413
                     /locus_tag="POA77_002646"
     CDS             14108..14413
                     /locus_tag="POA77_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6366049.1"
                     /translation="MKKTVIAATVLCLTAGTPIYSFAADPCEVVLCMYGKATGNSGGS
                     ECSSAERAFFKINAFKKHHRFNPGKTFDMRKEFLGECKAADPESISKILSKFGRVKG"
     gene            14447..14752
                     /locus_tag="POA77_002647"
     CDS             14447..14752
                     /locus_tag="POA77_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HDI6366050.1"
                     /translation="MINEDLFIKNIHSKNQDRISVAMVYDTLSKEAHRGCGLYYEIYE
                     SCFIGLLRDHLSELNEADANRLRRYAESKGTKIDDASYSEALEAERECRAEIYREQM"
     gene            15462..17321
                     /locus_tag="POA77_002648"
     CDS             15462..17321
                     /locus_tag="POA77_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HDI6366051.1"
                     /translation="MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFAS
                     MKSDERQKAPLWKKLIIAFSLLCFLSGTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRI
                     AFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLERELRTDVRTVKSLLPETLHYNPL
                     DYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILLYQSILAGE
                     GVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAG
                     FSLAEKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGK
                     IEELALLNAINSPEGFSLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRD
                     RVIDVPRPIAVYLSELKYHLSRSALEGLGAARDKGVHIIMDHQSIADLKDCPADLKGD
                     AVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDELRKAKTDNMLTETIDSERT
                     IRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVSPDIAAT
                     VAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF"
     gene            17331..18077
                     /locus_tag="POA77_002649"
     CDS             17331..18077
                     /locus_tag="POA77_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDI6366052.1"
                     /translation="MLISSFHERQTRNNEKIKQLLNFLKEETYSDFKTLMLLFGFRDH
                     KSLYSLLAKTERMGLIQKHVLESRTMKISLWGITSDGLAVVLTPDDKIFPPRFEPSKI
                     TGWTLEHHLDNQAARLILEKKGASGWINGDRATFLSQYQVKHRPDGLIILPGSTVIAI
                     ETERRLKTRARYQSIIASHLLARTRKLWIHVFYVIPDPQKKRALELLFDSIRHVIINH
                     QHVPLEERHRRVFRIYTLDELKRLSLDSYT"
     gene            complement(18271..19218)
                     /locus_tag="POA77_002650"
     CDS             complement(18271..19218)
                     /locus_tag="POA77_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDI6366053.1"
                     /translation="MTISLYTSRTSAPNTAAATSVSSLEQSGSSKTKFSKTRTDIYQT
                     VTDSIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMNIMLLWCSASKQGFSD
                     SRWMTYKQAQAAGGQVRKGEHGTTAIFYTTLEKENDAGEVEQIPMLKTFTVFNVQQID
                     GLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDEVWLPERHLFSD
                     AANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMADLGIEGE
                     VQHESYIASWLKALKNDKRYIFKAVSAASKAHSYLMDKL"
     gene            complement(19782..20143)
                     /locus_tag="POA77_002651"
                     /pseudo
     CDS             complement(19782..20143)
                     /locus_tag="POA77_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20285..21343)
                     /locus_tag="POA77_002652"
     CDS             complement(20285..21343)
                     /locus_tag="POA77_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366054.1"
                     /translation="MLFSYIYVPHKMEKMQAFIDFIFYEVWCKARVRGPFCLKLFEAN
                     AELYEVMEDFSSSDTQGAVFFYNHVEKIYGLFSSLTEVQIDQFKQWYQGNNDLEKICA
                     NDPSIQVVRYSDIAIHHKDIAEQLAVFFKGLYSQSLLDLAVLRAKIGDIHDHYQSFAA
                     VNKAGKCPFCGIGDIKGGNHSKREAYDHYLPKALYPFNSINFHNLAPACHECNSTYKL
                     SKDPIQSGALRRKAFNPFASVDHVIQLQITLQHANIDALEPADIIIQFGPDTLEEELE
                     TWKDLYGIEERYKAKVCAENDGKYWLTQVLDEWKEEGLSPTEFMRTLARQAKKKPYAE
                     CNFLKEPFLKACHRIGVF"
     gene            complement(21349..23133)
                     /locus_tag="POA77_002653"
     CDS             complement(21349..23133)
                     /locus_tag="POA77_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction system-associated AAA family ATPase"
                     /protein_id="HDI6366055.1"
                     /translation="MKLLRLKISDPSGFRSLPCGFEHYFRTEWDLQEELNQHEGFAPF
                     VCAGPNGSGKSNLLEALAAIFFQLEILRVRRSFLPEVLQSTDHDLSPISFELDYLIRV
                     PEEFRISGGQEWAKVSVWKNNGESVRFHWVNQSDFDTNADEVFKGSHADILLPQYVLG
                     YSSGENEILSLPFFKMRFVQFDEYWNALTRQLSYSGHPESRLAYLDSGFSQAILLCNL
                     LFQNETALQPFREDVGIEALREFRIIIRRSIPLAPEQLTSFASEDKNQHQSLDDILNS
                     NPALHVDMDEESGQSYHLNLMQLLEGDDKSSLVVSALKRCASLYYEDECNDTLILDYW
                     VNDATRQAFRENFNGSALALFQAFQVLLTLNLYKVSDNLKTDLYRSTSHYVSETVPTL
                     ASDERIMRFKFVRFTKQGVEEPMMLKELSDGEHQLLHSLGLCLLFRETNSLFLLDEPE
                     THFNPHWRASFITRMRQCLCNTENVEQEMLITTHTPFLISDSKPEKVLVFSKDKYSGA
                     VTISIPKYNTLGASINKITMNTFGKRETIGGHAQAVLDDLRRRFNEGIEDKETLITEI
                     DEQLGDSVEKVLLLKAIFDSDNPTNDEV"
     gene            complement(23130..24695)
                     /locus_tag="POA77_002654"
     CDS             complement(23130..24695)
                     /locus_tag="POA77_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6366056.1"
                     /translation="MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFK
                     KRIASSDTSKYRIVYKNELVVGFPIDEGVLGFQTKYPVGIVSPAYGIWKLKDESVCHI
                     PYLERYLRSSEARRLYASRMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKV
                     EGLIEQRKQLLQYLDDLLKSVFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQG
                     GKYKYIRMNNITPDGYWDFENLKYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAV
                     YDRDETVIIAGYLIRVRFDQQTNPWFVWGHLNSKFGKAKLFNLCRNIIGMANINAQEL
                     RAIPILKPPLELQNKFATIVEKAHAIKFRYQQSLADLETLYDVVSQKAFKGELELSRV
                     PIPTQIFFPVSGEEPEHGDAVQHIAVGNYLPVTNSLPSALDNAEARKSLIEEWLEAYI
                     AQLGNSSFSVHEFMMTAQARLAELYPDVDSKFGESDYEHIKAWVFKALAIGTLKQVFN
                     DADNCIELKAVLV"
     gene            complement(24692..>26347)
                     /locus_tag="POA77_002655"
     CDS             complement(24692..>26347)
                     /locus_tag="POA77_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDI6366057.1"
                     /translation="DLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKR
                     QDEGETSGEKYISKFAGTWIPPEERNRPVAEQHPIDKRTLRWSEFKKLQPEEMLQHVR
                     DKVFPFLKDLNGAESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDSQEKGQA
                     FQDIQGDVYEFLLSEIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFL
                     LGAYQYILTQLSLSQNLKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDI
                     DATMVRLGLMNLMMHGIDEPHIDYQDTLSKSYSEETKYDIVLANPPFTGSIDRGDINE
                     NLKLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKA
                     VITLPSGVFKPYAGVSTAILLFSKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNK
                     LEGYGDLQDIIQKYHSRDEASDTDRTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQ
                     YEQPEAILERLIQAEVGNVDKKVLNKVQSGILYELLELKGMIV"
BASE COUNT         7127 a         6462 c         6136 g         6622 t
ORIGIN      
        1 tatactagcc tgaactatat ttattcttct gcaatcaatg catacataac acaaatatca
       61 ctcaggtaca ctactcaaac cacgctggga tttttcctca agttataatc atcaccccga
      121 aaatcattcg gcatttactc attaaatagt caccccatag gcctgtacat gtttactcag
      181 aaatatacat ccttttctct gtcacaaacc ctctgattaa tcataaataa atacttgtga
      241 caccaatctt tttccttaac ggaacgaatt gttgtgtaga aggagataat attatgtcaa
      301 aaaaatatca gccattgctt ataactcatt atatgtcaac atgggtcact ataacggaag
      361 cagttgagat cactaccaaa gccataaaac aaaaaattac tcctagcgat atttatcgtc
      421 atgccttgag tggcaacatc ctactatcgg tctatttcca gtctcctgtg atacttaaga
      481 agatacaaac ttttaatgga aaaataaaat tcaggcagtt tgagggagac ctacttgata
      541 aactatgcat gcttgacagg gacggtttta tttatggtca aaatttaaga ctctgtacag
      601 aagccagata tgtttgtcct gtgcaacaaa ttatagatac ccccctgata ggctatgaat
      661 atgttttgat tcagcgcata ctggcacgtg aacttaaatt cccctcccca attgtggggg
      721 caagaaaaac taaccatggt attatagtaa gattttcaga ggagcttttt caaattttcg
      781 aaacgatgag ttggaaagaa cgcgtagaaa aacaaatttc cagactacct aaaaacacag
      841 cattagatgt cataaaaaaa ctaacagaag tgacgaccat aaaatataat cacaatgggt
      901 gttttcctct ttatacatta ccgcctgatg cctgttttgt tattcgccac accgaagtcg
      961 aaagactaat taatttatac aaaaaaaggg agagtcatcc catttcacct tcccgtatga
     1021 caacaccgtt atcacgcctg ttttggctgg cctgcaagca caatgatatt atcagcccac
     1081 tgctgaatca tccctataag ctcctttcta tttttgaaca atgggcatca ggcgatggca
     1141 tcggggaaaa actagacgct gaaacactga aaaatgcgct gaagcgtggt tcgccatctt
     1201 cgacatcctt atccggctaa taccatcccc atttgtaatg gccgacaacc ctaaataagg
     1261 gttttgcatc ccttatttag ggctcaccag ctttctctca ggcttccact gtgttttttt
     1321 attctccggc caccgtttat ttcagtcaca ggaggatata tgacatctta tcagttacta
     1381 cgcctcagac aggttgagca aaaaacaggg ctgaagcgtt cacagatata cctttatatg
     1441 aaagagggtg catttccccg ttcaataaaa ataggccctg ctagtgttgc ctggctcgag
     1501 tctgagattg acgagtggat caacaaaaag ttatctgacc gataaagacg caatgaggag
     1561 aagatgatga acattaatat cccggcactc ataaacttcg atcctgcagg tataaaaaaa
     1621 agcacctgca tgtatatttc agacctgccc ggtattgtgc tatatgacac tataccattc
     1681 actgaatatt cagctgaatt actgagtact tattcagtta aggagtcaat ttcatgcttc
     1741 catccctgaa tcatattata ttaaccgttg ctcttcaggc gctacgggaa ggaaatattc
     1801 atcactgcga aactatggga ttcacttacg atgaaatgaa tttgctcggt tgcttatcaa
     1861 taaacgatct tatcaccctc agtcaggcac ctttaccgct tgtcgatatt actatccgcc
     1921 atgatgtcct gcaaaaatta ctggcatcat cccacgagga aaacaggcgt caggaacaac
     1981 tgaacagagc tgtcaggctg gggggctcaa tcgcactgat gaaccgttat ttcggggttg
     2041 gctccagaga aacctgtgca cgccgccggt tactcggtgt ctccgttcca aacgggagaa
     2101 cacccattcc ggatgaagaa accgatgctg caatctggca tcagtggcaa aaatatcggg
     2161 tcgaaaacat tgattccccg gaggcgctgg acgccatgat gctggttaca gaaaaccttt
     2221 catcgtgtac tgccccctcg ctgaccgttg tctggaactg catcacgcat tgcgaaagaa
     2281 agaacacggt aaggagaata caacgtgcca gatgaaatcg atcgcgatca ggcgtttaat
     2341 gaacggtgcc tagaagcact gattgaacaa agccggttaa gacccacacc gacaccttcc
     2401 ctgcagcact gtcgattatg cggcaaggct attccggaaa aacgccgtca gacgctgccg
     2461 ggtgtaacaa cctgtactga ctgtcagtcg atactggaga aacgcagacg ttaaatctgt
     2521 atgtgcggag gagaatagct ccgcacattt taatatgcac cagccggctt gttttcaggt
     2581 tcccgtgcct ccatgtcatg gatgaataac ctgctcacat ctcctgctgt ggcaatcctt
     2641 ttttacagtc agtacaccag aggtcagaca tgaacaaacc ggcaaacaag atggaattcg
     2701 tcagaaaact gcggtaaata aatgacggca gcctgctgga aaaggttgtc gggcatatgg
     2761 aagaaaagct cacagggaat gagcttgaaa tgctttatgc cgcagcagat tatcgccgcg
     2821 cagagattgt aatgaaccag tactttgata aggttcccgg taatgtctgg aaatatgtca
     2881 gataactctg tgttttttat gcaacaggta cagacggata ccactgcggc atgaaaacga
     2941 atttcaccac ccggcccggt aagtatcggc ctaagtggcc aggaaacgtt taaccagatt
     3001 aaagaccgtc ccttacaaca aaagggacta ctgtattcct gtctttcacg ccttacacat
     3061 tattgtcgtt accaaattca gccacagaga cagcatacaa ccccatctga aatataaaga
     3121 aagtcttcct ccggatgttt aatttaacct gataataatc aacaaatatt attatcagcc
     3181 cgcccgtcat cattcatgca cacacacacc gtgccagtaa tacgttaatt tcacatactt
     3241 ttttatcatt ccttccggta tttattttcc cgttaaaatt tcattacccc tcctgcatcc
     3301 tgaccatgct ctttaacaat atggagcggg ctgtcacaca accataattc agggtgccgc
     3361 tgaatgttct cgctgacccg ttaaaaccgg aatgcaggat cggaatgtga tcgaagaaat
     3421 catcttcaac aattttaatt aaacgaggac actatgagaa agcctgttta ttacgcgtat
     3481 gttgcccagg aaagtcatcc ggactctcag ggtgagaaaa atacctggtg gacaaaggtc
     3541 ggagtcgcct ttccccacca cggtaaatcc ggtctgaata ttgtcctgac gcccggtatt
     3601 gccgtttccg gcaaactggt cctgcttgag ccccgggagg aaaataatcc accacagaat
     3661 acgggcccca tcgaaaccgt gtcgtaacca cccttaattc tgttcccacg gcatgcagta
     3721 tcagcagacc ctgcatgtca cttgtttact gaggtcacca tgctttccac cacagccttt
     3781 ctggcgatag ccatgcagtg cgccgccagc attcatccgt ccacggcatt cgacgtggca
     3841 cgggtggaat ccggttttaa cccttatgcc atcgctgaga ttgtcccgaa ggccggcccc
     3901 tctttcggcg atcaacacat tatttctcac ctgccagcca gccggggaga tgcagagcat
     3961 attctccgtc gcctgtcagc acaggatcgc cgttactctg tcgggctgat gcaaatcacc
     4021 agcaccaatt tccgccatta tggtgtgtcc gccacagagc tacttgatcc ctgtaccaat
     4081 ctgtccgtct tcgaacacat tctcagagac tgctaccggc gcggcggcac gctgaaaagg
     4141 gcgctgagtt gttattattc cggcaatttc agtaccgggc aacaacctga aacggcgttt
     4201 tccggaacga gctatatcca gcgaatcggt tatccgccgg gatcatcccg ctatgcggtc
     4261 cccggaaccc gtgaggacaa atcatcagtt gccgttcccc tgaaaaccgc ccctgccagt
     4321 gactcatccc gtcaccacgt gttctggccc ggagtcgtcg tccgtggcgt ccctgcgaaa
     4381 ctccgccaag aaaaaatctt catcgcacct ttctccgcgc cgtcagtgcg ccggagtttc
     4441 tccctgtcac cccgtgagga ggaggaataa tgatactttt ccgcaagcta aaagctgtgg
     4501 tcccccccgt ttcggcctgt acgctgacga ctttttctgc aaccccggca ctggccgacg
     4561 gatttcagcg ggcaaatact gtcatgcaaa aagtgtcaac cgggctgcat ggtctggcgg
     4621 caatcacgat caccgtggcc gtgatctgga ttggctataa gaccctgtgg aaaggcgaga
     4681 gcctgtcgca gtgcggctac atcatcattg gcggcatttt aattggcgga ggcagtgaaa
     4741 tcggcgctct gctgatgagc taagaggctg aacatatggc aaagctgctc aaggcaatga
     4801 aacgcccggc ggcgctgtgg ggggtgccaa tggtgcccct gctggctgtc acgggtgtaa
     4861 ccatcatcgt tgctatctgg acttccgtcg ctctcctgtt cttacttcct gtgcaatttc
     4921 tggtgatgaa gtcccttacc agaaatgaac cgatgcggtt caatctgatt gccgtatggc
     4981 tgagagcgaa gggaaagccg gtagcaaacc gtttatttgg cgcaacaaca tttatgcccg
     5041 ttgagcatga tgatgttgat atcaaggagt ttctcgacgc catgaaacta aatcagtgtg
     5101 ccaccatcaa gaagtacatc ccgtactcat cgcacattca tcagcatgtg gttcgttccc
     5161 ctaagagcga tctgtactgc acctgggaat tgatgggaac gccgttcgac tgcgagtctg
     5221 acgagtcgct ccagttcggg acgaaccagt tacacgggct aatccgctca ttcgaaggta
     5281 tgccagttac tttctatatc cataacgacc gcaatacgtt taccgacaat cttcataaag
     5341 attccggcaa cccgtatgcc gatgaagtta gccgtcttta ctacgcttct gtcggtctgt
     5401 tccggcgcaa ccgcctgttc ctgacggtct gctatcgccc gtttgtcagc cttgaaaaag
     5461 cagaacgcaa acggatgaaa gacagccaga aactaaagga gctggacggt gctttgctgg
     5521 aaatgctgga aataaaaagc acgctcgata ccgcgctgtc gcgctatggt gcgcgacctc
     5581 tcggtacgtt taccgaagga aacgcggtgt tttcgtcgca actggctttc tatgaatatc
     5641 tgctaacgca ccagtggcga aaagtgcgcg tcacccgtac cccggcctat gacgtgatgg
     5701 gggcggcggc gttgttcttt tcggctgaat ccgggcagat aaaccacgcc aacggtacgc
     5761 aatatttccg ggggctggag gttaaagagt tcgccgaaga gaccgccacg gggatgatgg
     5821 actcccttct ttacgccccc tgcgattacg tgatcacgca gtcctataca tgcatgtcac
     5881 gcgaggaagc gaagaaagcc attaagcgta cccgccgcct tttgatgagt gccgacgacg
     5941 atgctgtttc ccagcggctg gatctggatg tggcgcttga cctgctgaca tcggggaaaa
     6001 tcgcttacgg caagcaccat ttttccatca tggtgtattc cccgtcgctg gaaagcctgg
     6061 ttgctgatac gaatgaaatc agtaacgcgc tgaacaacat agggataacg cctgttccgg
     6121 cagaaatatc gctttcagcg gcgtatatgg cgcaactgcc aggcaactac aacctgaggc
     6181 cacgtaaagg ggagctgagt agccagaact ttgtggagct ggccgcgctc cataatttct
     6241 atccgggcaa gcgcgataaa gccccctggg gggatgcgat ggctttactg cgcaccccgt
     6301 caggcgatgg ctactatatc aacctgcata acacactggc agataaggac gaattcaacg
     6361 agaagaaccc ggccagtacc tgcatcctcg gtacgaacgg ttcgggtaaa accatgttga
     6421 tgacgttctt tgagatcatg cagcagaaat acgggcgcga ggatagcttc tccccggatg
     6481 ccaaaaccaa acggctgaca accgtttacc tcgataaaga ccggggcgca gagatgaaca
     6541 tccgggcgct gggtggccgt tactatcggg ttatcagtgg tgagtctaca gggtggaacc
     6601 cgttctcgtt gccagcaacc aaacgcaata tcaatttcat caagcaactg atgaaaatcc
     6661 tttgtacccg taacggctcg acgatctccc cacgcgatga acgccgtctg agtgatgcgg
     6721 tcaatgctgt gatgaatgac gagccgcaat atcgcgtcta cggcattacc cgaatgctgg
     6781 aaaatttgcc tgagccggct acgaaagaag cccaggaaaa cggccttagc attcggttgt
     6841 cacagtgggc gcagggcggt gagttcggct gggtgtttga taacgagtct gacacgttcg
     6901 atatcagcaa ctgcgataac ttcggcatcg acggaacgga gttcctggac gatgcaagcg
     6961 tatgtgcgcc aatctcgttt tatctgctgt accgcattac cagcctgctt gacgggcgac
     7021 gcctggtcat tttcatggat gaattctgga agtggttacg cgacccggtg tttaaggact
     7081 ttgcttataa caagctgaaa accatccgta agctgaacgg gatgctggtc gtcggtactc
     7141 aatccccggc agagatcatc aaagatgata tcgccccggc agtgattgag cagtgcggga
     7201 cacagattct ggcggcgaac cccaacgccg accgcgcaca ctatgttgac ggtatgaagt
     7261 ttgaaccgga agtgttcgac gtggtgaaag ggcttgaccc gcaagcccgc cagtatgttg
     7321 tggtgaaaaa tcagttcaag cgcggcgata ccaaacgttt tgccgctcgt gtcacgctcg
     7381 acctgtcggg tatcggcaga tacaccaaag tcatgagcgg cgatgccccc aacctcgaaa
     7441 tattcgaatc gatttaccgg gaaggaatgc agcctcatga atggctcgat acctacctgg
     7501 ctaaagcgct ctgacccaaa ggagatatac gatgaaaact cgctttcgta ctcttattct
     7561 ggcttgcgtc attgcaagcc cactggctca tgctggtatt cccgtcgctg ttgatgctga
     7621 ccctatgcgg gacgtgcagt gggcgcagga gttaaaacgc tggatggaaa cggccagaca
     7681 ctaccagtca caaatacagg catataaaga ccaactggcg acggccactg gcgttcgtga
     7741 tattgctgat tttgttgacc aggccaaagg cctgaaagct gaccttgaaa agctgcgtaa
     7801 gcccggacag gcactgaatg acctgcttct ctccggcggt tcctccgggc agttcgatgc
     7861 gttgtacgag aagtataaaa ttttcgacac ctgcaacact gcgcaatcgg ggagttatgc
     7921 caacgtatgc aagcagcagg tcatcaacaa ggctatccag cttgagcaaa ccgacgaggt
     7981 tcagaaccag gtaagccaga ccctggggga aataaacagt ctctccaaca gggtagcgtt
     8041 agccaaagat tcgaaagaat ctcaagacct tgcaaacagt attcagctaa aatctgtcat
     8101 gctgaacacg cttaccaccc aatgggaaat gagcgtgaaa gcagcagaga agcgcgaaaa
     8161 tgcgctagaa gcggagcgtg ttaagcagtg gaatcagcag cagttaaacg cgccaactgc
     8221 tgaccttaat aatttatgag gtatttatgt tatcaaaaaa aattatttta atttcgacga
     8281 tcaccctgtg cttttctatt gttggatgca aagaggaagc taaaacaaca aaatggtata
     8341 gggatcaccc tgacgaatta aaactagctt atgaaaagtg ccaaaagagt ggtgacgctt
     8401 ctgataattg caagaatgca aatgaagccc actatcaaat caagcagcta aacgcaccaa
     8461 ctccagactt aaataattta gaggagtaat atatgagtgg cggtcttttt gttggggttg
     8521 agaaatacct gataggagga attacagacg ccacaaagag attgatgatg tcgtactctt
     8581 ctatgatgat ggggttggca gcagcgtcag ctaccatcta catcatgtgg cgtggatacc
     8641 agactttggc tggtaagcta tctacgccta tggaagacac gatgtgggac atcatgagga
     8701 tggcgatcat cctgtccttc gtggctaatt taggcggcta cctcgatggc gttattgatg
     8761 ccataaacgg aataaaggag ggtttttctg gtagtgataa tatatggcag ttattagaca
     8821 ccctatggaa taaggctaaa gtgttaggga aaacgctaca tgacatggat aattctacat
     8881 atgtaaaaga tgagggaatg actgctcaat tttgtgtatg gcttggaata ttcgcactta
     8941 tgattctagc gggctttgtt tcaatggttg ccgaagtaat gattctacta ttaggaataa
     9001 ctgcacctat tttcatattc tgccttatgt atggtttcct ccgccccatg ttcaataact
     9061 ggttgcaaaa tatatttgca ggaatattaa caattctatt taccgctctc tcattgagaa
     9121 ttgtagtgaa ttacttgaat ctcgttctta ataaggcgac gaaggtagca gacagttcaa
     9181 caattgttga acttggtgct caagtctgcc ttgcaggtat tttcgctgca atactggtgt
     9241 atatttccgc taaggtagcc gcagctctgg ccggggcaag cgctaccgta tccatgcagg
     9301 ggctggccgc tgtaggtatc ggtgcggctg cttttggtgc cgggaagcta gccgggggtg
     9361 cagttaaaga aaccaaacgt tccgcccagg acaacattca gggctggaag gatgcgaatg
     9421 caggtaagcc cggcaagccc ggaggtgagt taggctataa cgcatctaaa gcgcggaagt
     9481 acgcgattga gcagatgata gcccggaacg aagcgcggag gctggcacag tcgagagaat
     9541 ccgcggttaa gaagttcaac agctaggcca gacgtctccc gtccatcccc ccgaaatatc
     9601 atcatatgaa aatataatta tattttcata tatctaattt agttgaggaa atcattgatg
     9661 aaacgcttga ttgcgctggc tttgctgtgc gcgctttctg gctgcgccca gaacacgcct
     9721 ccctctgcct ttagcggtaa gggagaaccc gtaaacaccc ctcaaattat ggcggagtta
     9781 aacagtcatg agtgaacaga aattaatagc tgaatcccgc acttttgagc agcaaatgat
     9841 tgagcgggat aaacgcgcca caaaagccgg ttttgttgtt ggcggtatgg gtctgctgat
     9901 tgcggtactg gcgctcgtcg ccgtggtcgt gatgttgccg ctgaaacaga cggacgttga
     9961 gctgtatacc ttggataacc acacaggacg ggtggaacac gttacccgga catcgaaaac
    10021 cagcctgacc gcaacagaag cctatcaaaa ggcgatggcc gcaaactacg tcaaagtgcg
    10081 tgagcggtat gtctatccct ctttgcagga cgactacgag acggtacagg tctacaacgc
    10141 tccccaggta aacgatgact atctggcgct ttacgccgga aaaaacgccc cagacaaggt
    10201 ttataagaac ggggcgcaca ccgtgaaagt ggagatcttg tccaaccaga tcaccgatgc
    10261 gacagcccct gatagggtcg ccaccatccg ctacaagaag attatccgcc gtctggctga
    10321 taacagcacc cgcaacgaat attgggatgc ccggttcacg ttccattcta accctgacaa
    10381 ggaaatgagc gatgcggaac gcgagatcaa ctacttcggc tttaccgtca ccagttggca
    10441 gaccgaccgc gaaatccggg gaggtgaatg atgaaaaagt taacgtttac cgcactggca
    10501 ctgatgatgt gcggcgcggc atgggctgcg gccataccgc aggcaagccg ctatgactcc
    10561 cgcgtgcagc aggttatcta taacccgcag aacgtcactg tcgttaacac caaacccggc
    10621 tttatgacaa cgctagtctt tgataacgat gaggcggtca taagcgccaa acctggcttt
    10681 gatgaggcgt gggaagccac gccggatgca aaccgggtca acgttcgccc ggtcgcgctc
    10741 acgcagggag cgcccggaga agatggcaac acaacgcagg tcgtgatccc cccgaacagc
    10801 cgggactggc ataccaatat gctggttgtc accagtaagc gcctgtacaa cgtcgagctg
    10861 aacgtcatcg atgataaatc ggcacaacaa cccgctttcc aggtgagcta tcgctatccg
    10921 ggcgaagaac gggacaaagc gagccgggag gcgacggcca gacagcgcga gtgggagcag
    10981 aaacagcaac aggccagcgt ccagaaagcg ctgaactcgg cgcagacgcc ccgcaactgg
    11041 agttacacca aatatccggg caaaggcagt ttcaacattg ttcccgattt tgcttacgac
    11101 gatggccgct ttacgttcgt gggcttctcc ccgtctaagt ccattccttc cgtcaccaaa
    11161 gagctgaacg gcaaagagca tgtggttaac agcagtatcc agaagaaagg cgatttcacc
    11221 gtactggtga ttcaggaggt gacgccacgc ctagtactgc gttccggtaa tgccgttgtg
    11281 ggtctggaaa atagcggatt tggcaaggta cacgccgccg atggttccac cgtatcccgg
    11341 caggtggaaa gagtggaaaa accggaatcc aattaaccta taaaaatatt tttataaaag
    11401 gaaacgtgca tatgaacgac gaaaacaaaa tgcctgtgcc ggatgaagcg caggcattcc
    11461 cggcacacgc tgaggatgat attgccacgc ttgagcggga agcccgcgct aaaagggagg
    11521 ccgagcttct caatgcgcag gacgacgaag agaaagaccc ggtacaaccc gccgttaaca
    11581 agctgaaaaa gcgcaaacgg ggcaaagcaa ccgcctttct tgccattgtc gcggtcacgc
    11641 ttatcttcct ggcatggggt ggtaactggg tttatcgcaa tatcctgtgg cagcctacgg
    11701 aagaaaagaa acaggacaca gccccgcaga cgaacaaaac cgattaccgg cagcgtaacg
    11761 atctcggtat gtccaccgat acagccgaag aagagccaga gcaacaggat aacggccaga
    11821 gcagcacggg gggaacaggg caggtggctc cggccgcccc gccagaactc aacaaagcca
    11881 gcttccttat tcgccgtgat ggttcggcca ccacacagaa cccggtaaaa acccggcagc
    11941 aggaaatgac gctgacatcg gcaacgacca ccggacaaca ggacagcagc gcggccacaa
    12001 ataccgccgc accgccgccg ggacaaagcc ccgcaccact gcgccgcata ccctataacc
    12061 ctgacctgta catcccggaa aatacctcga tcccctgctc actggatcgc cgctttgtat
    12121 ctgaccgggc ggggaaactt cgctgcacgg tcactactga catctggagc gccagcggca
    12181 acacgaagct gatagagaaa ggcaccaccg catcattact ttaccgcgcc atagcggaag
    12241 aaggtatgaa gcacgggcaa gggcgggcat tcatcattgc gacgaagcta cgcacccgcc
    12301 agccgcctta tctcgatatc ccgttggttg ataccagcgc ggctggcgag ctgggcgagg
    12361 ctggcgttga tggctggata gattcgcact tctgggaacg gttcggcggc gcgttaatgg
    12421 tcggcatgat ccccgatatt ggcgcatggg catccaacag cgcgggtaaa aaagaccgca
    12481 atacggacta tacggaaaac agccgccagg ctatggcaga tatggcacgt accacgctag
    12541 agaacagcat caatattccc ccaacgctct ataagaatca gggcgagatc atcaacctaa
    12601 tcacaggcga ggatattgat ttttccaaca tctatacact gaggttgaaa aatgaccgct
    12661 aagaatattt ccctcgaccg ctataaacag cgtttctttg gcgactttct agagcttccc
    12721 ggcctgacgg agatcgccgt caatcgtcca ggtgagctgt ataccaaaat caacggcgta
    12781 tgggagcagc acgccgttcc tttgtcctat gaggattgct acaactttgc ccggtgcctg
    12841 gcaaagcacc acggcgacaa tatcgaagat attaagccgg ggctgtcagc cacgctggaa
    12901 tctggcgaac ggtgccaggt gattgttccc ccggcctgtg agcgcgatac ggtgtctatc
    12961 accatccgta agccatcgaa ggtacaaatc ccgcatcagt catacatcga tgcgggattt
    13021 tataaccggg taacaggcga ggaaaaaacg gagacgcacg acgaggaatt aattgcattg
    13081 tacaatacaa aaaatattcc tctgtttatg gagaaatgcg tcgagtacgg taaaactctc
    13141 gcggtcgccg gggaaacaag caccgggaaa accacctata tgaaaatgtt gatagggtat
    13201 attcctgtac atcttcggat atcaaccatt gaggacaacc cggaaatcac gttctttatc
    13261 cataaaaact acgtccatct tttttatccg tcagaatcat cagatgaaaa aggttctatc
    13321 gttaccccgg cgagcctttt acgctggaat taccgtatga accctgaccg cattttatta
    13381 acagaggttc gcggtgcgga agcctgggac ttccttaaaa taacaggttc cggccatgaa
    13441 gggagtatga cctccattca tgcaggatca gcgaaagaag ctatagatgg gtttattacc
    13501 cgttgctatg aaaatccaca gtgcgcccag ctcccctata catttatgtt acgtaaggtt
    13561 ctggatagcc tggacgtgat tgtgagcatt gatttagacg gaaatgttcg ccgtatgaat
    13621 gacatctatt tccgtccaat tcatcgaaac cagtattttg aggaaatgaa agcatgataa
    13681 agaaacttaa agtcatcatg ttggcagcaa tagttaccgg atgttcatcg ccgccgcctc
    13741 ccgttcctgt ggagtgggat aaagccgctg ccccattaaa taccagttta tcgcaatgga
    13801 gtgacaataa cgttatagtc ccttccccga cagttaacgg taaatggaca tcatcaatca
    13861 gcgcacaaag tttctacgat acaatatgga ctccagcagt attttacgca gtcgcccatt
    13921 caacacgaat tgtcgtcact gcacaatccg gcactgattt ctttaatgcc aaaaactggc
    13981 ttcgccagaa tggcgcaaaa ggcgttattg aataccagcc tgtctttaat tgcctgacgt
    14041 gcagagaaac cactatttat ttctctcgct aatccattta agcaacacaa tctaataagg
    14101 atttatcatg aaaaaaaccg ttatcgcggc gactgtatta tgcctgaccg caggaacgcc
    14161 gatttattcc tttgccgctg acccgtgcga agttgttctt tgcatgtacg gaaaagctac
    14221 aggtaatagt ggtgggagtg aatgcagttc agcagaacgc gcatttttta aaatcaacgc
    14281 atttaaaaaa caccatcgtt tcaatccggg taaaacattc gatatgcgaa aagaattttt
    14341 aggggagtgc aaagccgcag accctgaatc aataagtaaa atcctttcaa aatttggcag
    14401 agtgaaagga taactgacat atccattttg atatgagagg ttcgaaatga taaacgaaga
    14461 tttattcatt aagaatatac actcaaaaaa tcaggacaga atatctgtag ccatggtata
    14521 tgacacgtta tcaaaagaag cccacagagg ttgtgggctg tattacgaaa tatacgaaag
    14581 ctgttttatc ggtcttttgc gcgatcatct ttcagaactt aatgaagctg acgcaaatag
    14641 attaagacgc tatgccgaga gtaaaggtac gaaaattgat gatgcgtcct acagtgaagc
    14701 actggaagct gaaagggagt gcagggctga aatataccgc gagcaaatgt aatcacacaa
    14761 aacaaaagtg tattgtacaa tacagagact aacaggcgac gggattttaa ggcttcatta
    14821 tttttggctt caatttcgaa gaaattgtta agacgaaaaa taatgaagtg gaacgtggac
    14881 agaaaattca atttcggaga aattgctaat tttcaggatc cacgtccacc cgcgaacatt
    14941 cgccccagcg aattgaagcg ggccgggggg agccgcaaaa attttgccgc cccagcgagc
    15001 aaaaaggcac gcccacgtgc cggtttttgt ggggggcttg ccccgcacac cgtcgcagcc
    15061 aggctatatt tcccgccccg gcggggaata tgccgattag gcgcgaccaa cccctttaaa
    15121 gcagcgttcc cattttttcg agcttgcgaa gaaaaaatag gctaaacgcg cgtcttaaag
    15181 gggttggtcg cgcgtagcgt gcgacggtgt gccgcctttg acccaggggg ttttggcgtg
    15241 gcgtccagcc gcgacattac ccccatgcga caggcaataa gggcgtccag cccgcatgta
    15301 ctggcgcacg ccgtttcgtg cagggaagca tgctttccgg tacatggccg cacagcggtt
    15361 accttcttta ccccatacgc tcgccccgtc ttttcgcccg acaccgtggg gcgaattgat
    15421 ggggcgatgt catggggcga atcgctgacg aaggaggcat gatgatttta aataagctta
    15481 ccgcttcact gtccccgata gtgaacggga tgctggcggt gctggctttt gtgcaacaga
    15541 aacaactggt gctggcatta ttagcggggc tgacgatgcc gttttttgca tcaatgaaaa
    15601 gcgatgaacg gcagaaagcg ccgctctgga aaaagctgat tattgctttt tccctgctgt
    15661 gttttctctc cggcacactg gctccggtcg ttatcggatc ctttcagtgg ctgtataaga
    15721 tcagacccac cagtgataat acggtactgg cgtggtcagt acggattgct ttcactgtaa
    15781 ccgggattat ttttcatatt atgcttcgcc ggatattcac accggaactg gataaaataa
    15841 agaaacgcct tgttaaaaag accacccttg aacgggaatt gcgcaccgat gtccgcaccg
    15901 taaagtccct gctgccggaa acactgcatt ataacccgct ggattatatc gacctgaata
    15961 aaggcatttt taccggcatg ggcagggaaa atgaaccaat gtatctcccg ttaaaagact
    16021 ggcagaaaca acacgcggat attatcggca ccacaggggc cggtaaaggg gtagccgccg
    16081 gcatattact ttatcaaagt attctggccg gtgaaggtgt ttttgttatg gaccccaaag
    16141 acgatgaatg ggcaccgcat ctttaccgca aagcctgcga ggatgcaggc aagccttttg
    16201 ccttaattga cctgcgaaaa cagcaatacc agttaaatct gattgaagat atcacgcccg
    16261 atgagctgga agaattgttt gttgcaggtt tcagcctggc agaaaaaggg caggaatctg
    16321 atttttatcg cattgatgac cgaaaagccg cccgtatagc cgcacaattt gtcagcgata
    16381 atccatcgac aacaatccgg gatgtttata acggtgatta cgttcagagt attgccgaaa
    16441 agatcaaagc ctttttcgga aaaattgagg aactggcatt actcaatgcc attaactcac
    16501 cagaaggctt ttcgcttaag cacatatttg atgagggcgg ctgctgttat gttgtcggct
    16561 ccatgcgaaa cagcaaaatt attaccgccc agcgcatgct actggttcgc ctttaccagc
    16621 tggcagaaaa gcgcgatcga gtaatagacg tcccccgccc cattgctgtt tatctttccg
    16681 aactgaaata ccatttatcc aggtcagcac tggagggatt aggggcagcg cgtgataaag
    16741 gcgtgcatat tattatggac caccagtcca ttgccgattt aaaagactgc ccggcagatt
    16801 tgaaaggtga cgccgttgtc ggtgcggtcg ttgaaaatgc caaattcaaa ctggtttacc
    16861 gggttatgga cccggataca gcggaatggg tagcgagaat gtcaggcact attctggtgg
    16921 atgacgaact ccgtaaggct aaaacagata atatgctcac ggagaccatc gacagtgagc
    16981 gcacaatccg ccaggccgaa cgtttcttta ttgacagcaa tatgatcctg aacctgcccg
    17041 attttgtcag ctttatcttt acgacaaaaa cgctaccttc tgcttcactg atctcgccga
    17101 taaaggtcag aaagcgccag ctaaaaactt tttcggtttc ccctgatatt gccgcaacgg
    17161 tagcccccgt gaagatcatg ctggattttt gcgaggatga taaacccgcc atgtcggatg
    17221 tgatgacaac acacccggct cttttcttcg acgaaagcga agtcaaaccg gtaaagccga
    17281 aacctgatga tgaagaacac ctgtccccgc tggattttta aggagcgctt atgctgattt
    17341 catcatttca cgagcgccag acgcgtaaca acgaaaaaat aaaacaactg ctgaacttcc
    17401 tgaaagaaga aacttacagt gattttaaaa ctctgatgct gttatttggt ttccgggatc
    17461 ataagtcgct ttattcgcta ctggcaaaaa cagagcgaat ggggttaatc cagaagcatg
    17521 tgcttgaatc acgaacaatg aagatttcat tatgggggat caccagcgac gggctggcgg
    17581 tggtgttaac cccggatgat aaaatctttc ctcctcggtt tgaaccatca aaaatcaccg
    17641 gctggacgct ggaacaccat cttgataatc aggccgccag gcttattctg gagaagaaag
    17701 gcgcgtcggg atggattaac ggcgaccggg ccacctttct cagccagtat caggtaaaac
    17761 atcgcccgga cgggttgatc atcttgcctg gcagcaccgt gatcgccatt gaaaccgagc
    17821 gtcgcctgaa aacccgagcc cgctaccagt cgattattgc cagccacctg ctggcccgta
    17881 cccgcaaact gtggattcat gttttttatg tcattcctga cccacagaaa aaacgggcac
    17941 ttgagctcct atttgacagc atcagacatg tcatcatcaa ccatcagcac gtcccgctgg
    18001 aagaacgcca ccgtcgggta tttcgcattt atacccttga tgaattaaag cgcttgtcat
    18061 tagacagcta tacgtaagct tttactcagg ccatcgtgat ggtctcaccg ggctggcggt
    18121 aataccgcca gcccggtata tttttattcc actgcgaggc gcagactgtt cggtactcgc
    18181 tatgctgcgt tccgcctcag tctgcgccct gcagcgttgc cggttaaaaa ccatgtaacc
    18241 gcattccctt gcggttgtct ggctccgtcc tcagagttta tccatcagat aagaatgcgc
    18301 tttagaggcg gcgctgaccg ctttgaaaat ataacgcttg tcgtttttca gcgctttcag
    18361 ccaggaagca atatagcttt catgttgtac ctctccctca atgccaagat cagccatcag
    18421 aaaggcactt cccagctccg cgacaagttc ttcctctgca taatccgcac tgccaaactt
    18481 ccctttcatt tcacggttca ggcgtttttt acccccgctc cagtgaacca gctcatgcag
    18541 accggtagcg tagaagttag ccgcatccga aaacaaatgg cgctccggta accagacttc
    18601 atcagttgat gggctgaaaa aggcgttttg tcctttctcg atgatgtttg cgccactctt
    18661 ccgtaacagg ttctcagcct gcggcaacgg gttaaatgta gcttccgggc tgactgtttc
    18721 agttgtcagg ggcaaaccgt cgatttgctg aacgttaaac acggtgaaag ttttcagcat
    18781 cggaatttgt tcaacctctc cggcgtcgtt ttccttttcc agggtcgtat agaaaatggc
    18841 tgttgtgcca tgttcaccct tgcgaacctg tccacctgct gcctgtgcct gtttgtaagt
    18901 catccagcgg gaatcgctga aaccctgttt agatgcgctg caccacagca acatgatatt
    18961 cattccgctg taagccgtgc cagtggcgta attggatggc agtccagaca tgccggatac
    19021 acgcagccac gggcaggacc acggttttac accggcttcc agcgctgcaa tgatgctgtc
    19081 ggtaacggtc tgataaatat ccgttctggt tttagaaaat ttcgtttttg aggagcctga
    19141 ctgctccagc gaggatacgc tggtcgccgc tgcggtgtta ggggcgcttg tccgggaggt
    19201 ataaagggaa atcgtcatgg ttttttctcc gtcagtgtgt gattttcgtc ttcgtatcgc
    19261 ctgacggttc gctttcccgg catcgttctg tcctgcaagg gcgcagtatg cgtgcaattt
    19321 tacccttgcg ggacgggttg tgttgggaaa cgattaaccg gaagcagata tgaagaacgg
    19381 aaaagcgcac gggagagaaa acctgcccct gcatagccgg gcttaacgcc ccttacttcg
    19441 cagcggtgaa attagattgg tggtaatgag tcgggaaatg gcattaacag gaggttcttg
    19501 ttgatgttag catgctttcc tgccggacga ggtgatactg ctaaccaggg gcgttttgtt
    19561 cttaaaatgc ctcgttccgg catcaatcac taccgagtcc ctgccgtccc cttattgagc
    19621 gccgcgtaca gtttattcac taccacccat agtgagcgat ttagtgaaac aggccacagc
    19681 gccgtaaccg cgggcatcat atgccgtagt ggcgatctgt cttattctca gtgcccctct
    19741 atcagtatgc gcccatgcaa actgacattc cccctgtatt atgttgacag ggagagacct
    19801 gccagcctac tggcgtgacg ttgcgacaga cccgttgcct cacacatgac ttccacagct
    19861 tcccgcttct ggtctgtcgt cagaactttc ggttcagggc cacctgcaac gcttctttat
    19921 ccatcatgac ttctgcaagc aactccttga ggtgggcgtt ctcttcttca aatgacttaa
    19981 gacgtttcac ctcaggcatt tccatgccat caaacttctt gcgccaagtg tagtacgcag
    20041 catcagagat agcgtgcttg cggcaaagct cccgggcaaa aactccagat tcagcttcgc
    20101 ggaggatact gatgatctgt tcgtcggaaa aacgcttctt cattatgatg tcctcatgtg
    20161 actaatgaag acgttactaa catcggtgta tgttaatcaa tcgggagcag ggaaaagcca
    20221 ccgtttgatg tttttttata acagtaagtt ataaacaaat tgatagcttg atatgtattg
    20281 agcctcaaaa aacacctatt cgatggcaag ctttaagaaa aggttcttta aggaaattgc
    20341 attcagcata aggcttcttt ttcgcttgac gtgctaatgt tctcatgaat tcggtaggac
    20401 tcaacccctc ctccttccac tcatctagca cctgagtaag ccagtattta ccgtcatttt
    20461 ctgcacaaac tttggcttta taacgttctt ctataccgta caagtctttc catgtttcta
    20521 gttcctcctc cagcgtatcg ggaccgaact gtataataat atcagcaggc tcaagcgcat
    20581 cgatatttgc atgttgtaga gtaatctgta attgaattac atgatccact gaggcaaatg
    20641 gattgaaggc cttacgacga agagcgccgc tctgaatagg atctttggat aacttatatg
    20701 tgctattgca ctcatgacag gccggagcaa gattatggaa atttatcgaa ttaaatggat
    20761 ataaagcttt gggcaaataa tggtcatagg cctcgcgctt gctatggttc ccacccttta
    20821 tatctccaat accacaaaaa ggacatttac ccgccttgtt cactgctgca aaagattgat
    20881 agtgatcgtg tatatcaccg atcttggcac gcagtacagc gagatcaagc agtgactgtg
    20941 agtataagcc cttaaagaaa acagcaagtt gttctgctat atctttgtgg tgaatggcaa
    21001 tatcggaata acggacaact tgtattgaag ggtcattagc acatattttt tccagatcat
    21061 tattcccttg ataccactgt ttaaactgat cgatttgtac ctcagtcagt gacgaaaaca
    21121 agccataaat tttctctaca tggttataaa agaaaacagc accttgtgta tccgaggaag
    21181 aaaaatcctc catcacttca tacaattctg cgttagcttc aaacagttta agacaaaaag
    21241 gccctcttac ccgcgctttg caccatacct cataaaaaat aaaatcaatg aatgcctgca
    21301 tcttttccat tttgtgtggc acatagatat atgaaaacag catcagtgct aaacctcgtc
    21361 attcgttgga ttgtcgctat caaaaatagc cttgaggagt aatacttttt ccaccgagtc
    21421 acccaattgt tcatcaattt ctgtgatcag agtttccttg tcttcaatgc cttcgttaaa
    21481 acgtctgcgt aaatcatcca gtactgcttg cgcatggcca ccgatagttt cacgtttgcc
    21541 gaaggtattc atagtaattt tattgattga tgcaccaagt gtgttgtatt taggtatgct
    21601 tattgtgacc gcaccgctat atttatcctt agaaaaaacc agtacttttt cgggtttact
    21661 atctgagata aggaaaggtg tatgagtagt gatcaacatt tcctgctcca cattctcagt
    21721 gttacataaa cattggcgca tccgagtgat aaaactggca cgccaatgtg ggttgaaatg
    21781 agtctccggt tcgtctaaca gaaacaggct gttggtttct ctaaacagta aacatagacc
    21841 taggctatga agcaattggt gttcaccgtc cgagagttcc ttgagcatca ttggctcttc
    21901 cactccttgt ttagtaaaac gcacaaactt gaagcgcata attcgttcgt cggacgccaa
    21961 cgttggtacc gtttcactga catagtgact tgttgagcga tacagatcag ttttgagatt
    22021 atcactcacc ttatacaaat tgagtgtcag caatacttgg aacgcctgga atagagccag
    22081 tgccgaacca ttaaaattct cgcgaaaagc ctgtcgagta gcatcattaa cccagtagtc
    22141 cagaattaga gtgtcgttac actcgtcctc gtaatacaag ctagcgcagc gtttaagcgc
    22201 gctgactacc aaggaggatt tgtcatcccc ttccagcagc tgcatcaggt ttaaatggta
    22261 actttgacca ctttcttcat ccatatccac atgcagagca gggttactat tcaaaatgtc
    22321 atcaagtgat tggtgctggt ttttatcctc gctggcaaac gaggtcaact gctccggcgc
    22381 aagaggaata ctgcgacgga taataatacg aaactcccgc aacgcctcta taccaacatc
    22441 ctcacggaaa ggttgaagcg cagtttcgtt ttgaaaaagc agattacaaa gtaaaatagc
    22501 ttggctgaaa ccactgtcaa gataggccaa cctcgactcc ggatggccag agtagctcag
    22561 ttgtcgagtc aaggcattcc agtactcgtc gaactgtaca aagcgcattt tgaaaaacgg
    22621 tagactgaga atttcattct cgccagacga gtagcccaac acatactgag gcaatagaat
    22681 atcggcatgg cttcccttga aaacttcatc agcatttgtg tcaaagtcac tctgatttac
    22741 ccaatggaaa cgcacagatt ccccgttgtt tttccacacg gagacttttg cccattcttg
    22801 tccgccagag attcgaaact cttctggtac tcgaattaga taatccagct caaatgagat
    22861 tggtgataaa tcatgatcgg tactttgcaa tacctcaggc aaaaaacttc gacggacccg
    22921 caaaatttcc aactgaaaga agatagctgc taatgcttca agcagattgg atttaccact
    22981 gccgttggga ccagcacaca caaaaggcgc aaagccttca tgttgattca attcttcctg
    23041 taaatcccat tcggttctaa aatagtgttc aaaaccacag ggcagactac ggaagccgga
    23101 cggatcagag atttttaggc gtaatagctt cataccaaaa ccgccttaag ttcaatgcaa
    23161 ttgtcagcgt cattgaatac ctgcttcagt gtaccaatag ccagagcttt gaacacccaa
    23221 gctttgatat gctcatagtc actttcacca aacttagaat caacgtccgg atataattcc
    23281 gccagccgtg cctgcgccgt catcatgaac tcatgtactg aaaacgatga gttgcccaat
    23341 tgcgctatat aggcctctag ccattcctcg atcagtgact tgcgagcctc tgcattatcc
    23401 aacgcggatg gtaagctatt agttacgggc agataattac ccactgctat atgttgtacg
    23461 gcatccccgt gttcaggttc ttcacctgat accgggaaaa atatctgtgt cggaataggc
    23521 acacgcgaaa gttctaattc ccccttgaaa gccttttggc taacaacatc atacagggtc
    23581 tcaagatcag caagactttg ctgatagcga aatttaatag catgcgcttt ctctacaata
    23641 gtagcgaatt tattttgtaa ctccagtgga ggcttaagaa tagggatggc ccttaactct
    23701 tgggcattaa tgttagccat accaataata ttacgacaaa gattaaataa ttttgctttt
    23761 ccaaactttg agttcaggtg cccccataca aaccaagggt tagtttgttg atcgaaccta
    23821 acacgaatga gatagccagc aataataaca gtttcatctc gatcgtaaac cgccgtttta
    23881 ccaaccaatt cttttgaatt ggtccggttg aaaacgagat ctcctttctg aagagaatat
    23941 ttatccagat ccttattatc tacatcgata tatttaagat tctcaaaatc ccagtaacca
    24001 tccggagtaa tgttattcat ccgtatgtat ttatattttc caccttgcgc tgatacactt
    24061 gtcccgtatc ttacatcgac agctaactca cctattgttg ttagttccca tccttttgca
    24121 ttttttaccg gatcactaaa catatcgacg aatacgctct ttagtaggtc atccaggtat
    24181 tgcaaaagtt gtttacgctg ttcaattagc ccttctacct tggccaataa attagctata
    24241 cgagcctgat cattgatggg aggaaaaggt acttccaaac taagaaaatc agacttcgtc
    24301 aagctacgtc tacgtgcaac aaccccttgc atacgagatg catagagtcg ccttgcctcg
    24361 gaggagcgca gataacgctc aaggtaaggg atatggcata ccgattcatc cttgagtttc
    24421 cagataccat aagctgggct aacgattcct accggatatt ttgtctggaa tccaagaact
    24481 ccctcgtcaa taggaaaccc aacaacaagt tcattcttgt atacaatacg atatttagac
    24541 gtgtctgaac tggcaatccg cttcttgaat ttgtctgatt ggtcaaccaa accatttttc
    24601 atggttattg ataggaccgg aagctctcga ttgcctgcac ggtggtgacc ggattgagtt
    24661 aatatgtcac caaggggaat agtcattcta ttcatactat catccccttt aactctagta
    24721 gctcgtacaa gatgccactt tgtactttat taagcacttt cttatccaca ttccccactt
    24781 ctgcttgaat tagtcgttcc agaatagctt caggctgttc gtactgcact tcttcaaata
    24841 tttcttcctt atagcgagac agcgaaagat cgtaattctc cgcttcaata tctattcttg
    24901 gcaccataaa acacttagct gtacgatcgg tgtcagacgc ctcatcacgg gaatggtatt
    24961 tttgaatgat gtcttgcagg tcgccgtagc cttcaagctt atttcgtttg tcatccaatg
    25021 agtaaccatc ggaagacatc tcataaaacc agacataatc ggtagcaggt ttggaaattt
    25081 tatcccccgg cccgaaaact ttggagaaca ataaaatggc agtacttact ccagcgtagg
    25141 gtttaaacac tccactgggc agagtaatga cagccttgag gtcgcagcgc tcaactaaag
    25201 tttgccgtag ttctttgaat gccttgcccg agccaaacaa tactccttga ggcacaatca
    25261 cacaggcggt accgcccttt ttcagcaaac gatagatgtt ctcaacaaac aaaagttcgg
    25321 ttttagtggt tgagagtttc aaattctcat taatatcccc cctgtctata ctgccagtaa
    25381 aaggcgggtt tgctagcact atgtcatatt tggtttcttc actatagctc ttgcttagtg
    25441 tgtcctgata atcaatatgc ggctcatcaa ttccatgcat cattaagttc attaggccta
    25501 aacgcaccat agtagcgtca atgtcataac catacagact ctcttgcaaa agaatccggg
    25561 cctttttggt cagagcggca gtcacagaag tgcgaaagaa cccatcttca tcatgagtag
    25621 atccttttga attatctctt tttaagtttt gagataaaga caactgagta aggatgtact
    25681 ggtaggcacc taacagaaac ccccccgtac cgcaggcagg gtcggcaata cgttgtccca
    25741 gttgcggctg gaccagatct gccatcaatt taataatgtg gcgtggtgtg cgaaattggc
    25801 cgttcttgcc tgcggtggcg atttccgaca gcaggaattc atatacatct ccttgaatgt
    25861 cttgaaaagc ttggcctttt tcttgagaat cccttttcat catctcgaag atttcgtcaa
    25921 tggccttcac tgcctccacc aacaatgctg actttgggat aatgaatact gcgtttttca
    25981 tgtgatgagt gaagttagat tcagcaccat tcagatcttt cagaaaagga aaaactttgt
    26041 ctcgcacatg ttgcagcatc tcttcgggct gcaatttctt gaactcgctc cagcgaagcg
    26101 tgcgtttatc tatagggtgt tgttccgcaa cagggcggtt tctttcctct ggtggaatcc
    26161 aagtaccagc aaacttagaa atgtatttct caccactcgt ttcaccttcg tcttgccttt
    26221 tatgatcgag ttcatctagc cgtttcataa acaacaggta ggtaatctgt tcaatggctg
    26281 tgagcggatt gctaatgccg ctactccaga atttatccca gaggcgtttg atcgcgcttt
    26341 gaaggtc
//
LOCUS       DAMZII010000067        26277 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZII010000067 DAMZII010000000
VERSION     DAMZII010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.067"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..388)
                     /locus_tag="POA77_002656"
     CDS             complement(<1..388)
                     /locus_tag="POA77_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366058.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLEC"
     gene            complement(427..1656)
                     /locus_tag="POA77_002657"
     CDS             complement(427..1656)
                     /locus_tag="POA77_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6366059.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1822..2184)
                     /gene="ssrA"
                     /locus_tag="POA77_002658"
     tmRNA           complement(1822..2184)
                     /gene="ssrA"
                     /locus_tag="POA77_002658"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2399..2881)
                     /gene="smpB"
                     /locus_tag="POA77_002659"
     CDS             complement(2399..2881)
                     /gene="smpB"
                     /locus_tag="POA77_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI6366060.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQMDKARIMKNAHR
                     "
     gene            3052..3489
                     /gene="ratA"
                     /locus_tag="POA77_002660"
     CDS             3052..3489
                     /gene="ratA"
                     /locus_tag="POA77_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDI6366061.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3479..3769
                     /locus_tag="POA77_002661"
     CDS             3479..3769
                     /locus_tag="POA77_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDI6366062.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(3831..4172)
                     /gene="bamE"
                     /locus_tag="POA77_002662"
     CDS             complement(3831..4172)
                     /gene="bamE"
                     /locus_tag="POA77_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDI6366063.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(4321..5982)
                     /gene="recN"
                     /locus_tag="POA77_002663"
     CDS             complement(4321..5982)
                     /gene="recN"
                     /locus_tag="POA77_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI6366064.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6068..6946)
                     /gene="nadK"
                     /locus_tag="POA77_002664"
     CDS             complement(6068..6946)
                     /gene="nadK"
                     /locus_tag="POA77_002664"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDI6366065.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7069..7662
                     /gene="grpE"
                     /locus_tag="POA77_002665"
     CDS             7069..7662
                     /gene="grpE"
                     /locus_tag="POA77_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI6366066.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(7717..9003)
                     /locus_tag="POA77_002666"
     CDS             complement(7717..9003)
                     /locus_tag="POA77_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6366067.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(9024..9815)
                     /locus_tag="POA77_002667"
     CDS             complement(9024..9815)
                     /locus_tag="POA77_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDI6366068.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            9982..11343
                     /gene="ffh"
                     /locus_tag="POA77_002668"
     CDS             9982..11343
                     /gene="ffh"
                     /locus_tag="POA77_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI6366069.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11480..11728
                     /gene="rpsP"
                     /locus_tag="POA77_002669"
     CDS             11480..11728
                     /gene="rpsP"
                     /locus_tag="POA77_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI6366070.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            11747..12295
                     /gene="rimM"
                     /locus_tag="POA77_002670"
     CDS             11747..12295
                     /gene="rimM"
                     /locus_tag="POA77_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI6366071.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            12326..13093
                     /gene="trmD"
                     /locus_tag="POA77_002671"
     CDS             12326..13093
                     /gene="trmD"
                     /locus_tag="POA77_002671"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI6366072.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13135..13482
                     /gene="rplS"
                     /locus_tag="POA77_002672"
     CDS             13135..13482
                     /gene="rplS"
                     /locus_tag="POA77_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI6366073.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13559..14041)
                     /locus_tag="POA77_002673"
     CDS             complement(13559..14041)
                     /locus_tag="POA77_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDI6366074.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14057..15283)
                     /gene="dgcN"
                     /locus_tag="POA77_002674"
     CDS             complement(14057..15283)
                     /gene="dgcN"
                     /locus_tag="POA77_002674"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDI6366075.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(15273..15791)
                     /locus_tag="POA77_002675"
     CDS             complement(15273..15791)
                     /locus_tag="POA77_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDI6366076.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(15941..16306)
                     /locus_tag="POA77_002676"
     CDS             complement(15941..16306)
                     /locus_tag="POA77_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDI6366077.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            16516..17586
                     /gene="aroF"
                     /locus_tag="POA77_002677"
     CDS             16516..17586
                     /gene="aroF"
                     /locus_tag="POA77_002677"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDI6366078.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            17597..18718
                     /gene="tyrA"
                     /locus_tag="POA77_002678"
     CDS             17597..18718
                     /gene="tyrA"
                     /locus_tag="POA77_002678"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDI6366079.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(18761..19921)
                     /gene="pheA"
                     /locus_tag="POA77_002679"
     CDS             complement(18761..19921)
                     /gene="pheA"
                     /locus_tag="POA77_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDI6366080.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(19943..20068)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(20020..20067)
                     /gene="pheL"
                     /locus_tag="POA77_002680"
     CDS             complement(20020..20067)
                     /gene="pheL"
                     /locus_tag="POA77_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDI6366081.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            complement(20171..20512)
                     /gene="raiA"
                     /locus_tag="POA77_002681"
     CDS             complement(20171..20512)
                     /gene="raiA"
                     /locus_tag="POA77_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI6366082.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(20783..21520)
                     /gene="bamD"
                     /locus_tag="POA77_002682"
     CDS             complement(20783..21520)
                     /gene="bamD"
                     /locus_tag="POA77_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDI6366083.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            21655..22635
                     /gene="rluD"
                     /locus_tag="POA77_002683"
     CDS             21655..22635
                     /gene="rluD"
                     /locus_tag="POA77_002683"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDI6366084.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            22632..23363
                     /gene="pgeF"
                     /locus_tag="POA77_002684"
     CDS             22632..23363
                     /gene="pgeF"
                     /locus_tag="POA77_002684"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDI6366085.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            23493..26066
                     /gene="clpB"
                     /locus_tag="POA77_002685"
     CDS             23493..26066
                     /gene="clpB"
                     /locus_tag="POA77_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI6366086.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6512 a         6141 c         6854 g         6770 t
ORIGIN      
        1 tgcattctaa tgagaataat aatagagttg tgctgtatat aaagccagag ttagttaata
       61 actttaaatt taatgcttca ggattttcac tgagaaagtg aaaggcataa actaagtata
      121 cagctaaagt agccgttcca attgttggtc caatatcgcc ttgatcatga ggttcaaggt
      181 ttacattgaa cttgaagatt agccattcaa atccccaagt aaaaaaaaca actaatatca
      241 tggctgcgaa taatttcgcg atgttatctg ctgatggatt catcagaaca gattgtttct
      301 gaaaaaattc acaaagtgag agtccgaaaa aaataaaaaa aagtggtctg gaactaaatt
      361 tttcaaaaac tttgaatagg gtttccattt tctttacctt caggattata ttagctattc
      421 tgatacttag gctaccagag catttgatct ttgagcttga acgtaatcac tccaccattg
      481 catcaaactc tgtcgctcta tcagatattc tgcacgattg tatgctgcga taatttcatc
      541 ttttttcgag tgggcaagcg ctgcctcaag aacttcagct ctgaatttac cagactcctc
      601 tgccgctgtt cgtgcaatag aacgcatacc gtgagctaca agctcgcctc cgaaccccat
      661 tcggatgata gctgcgttgg ctgtttgttc atgcatatga ttaagaggcg cttttatgct
      721 ggggaaaacc cattctctat gcccacttat tgatttcatt aattcaagga tgcgcaaagc
      781 ttctttactc aaaggaactt tgtgaagctt tttcattttc atgaaatcag caggaatgtt
      841 ccaaatgctg ttggttgtat caatatcaga ccaccttgcg cgaacggctt cacccggacg
      901 aacccatgtc aacaattgcc attcaattag catacgtgtt tccaaccgga ttgacgcatt
      961 cgtcaaagat tccataaacc ttggcaattc gcttggggga agggcaggca tattttgctt
     1021 ttttggttta ctgaatcttt gaccaaggtt gtcagccggg ttgaactcaa taagttcttc
     1081 agtagctgcc caccggaaga tttcattcag acgtgaaatg atacggcgta gagtttccaa
     1141 tacgcctcgt tgctcaatag gatcaaggtg ttgttttaag agcttaggtc ggatctcatt
     1201 gatagggaca ttacccagac cgggaaagac atttctctct aagctgcgcc agatgtctgc
     1261 tgcatggtct tgtgagatac ctgatgtctt taccttctca tctaaccatt tccgcgctac
     1321 ggcttggaga gtgtgctcag tagcactctt taatgccttc gccttatcgt tgttatggat
     1381 ttggggatca acaccatttg ccagaaagga gagatattca tcacgtaagg ctctggctct
     1441 tgcaagggta aggtgaggat atgtcccaag gctcattttg gttcttttct tgctcactgg
     1501 tactgcatac ctgaaatacc aatttttctt gcctcctttc gccaaaggag cgattcgtag
     1561 aatcagacca tcaccgtcaa acaagttgat ttctttatcg gctggcttgg tgcttttgat
     1621 ttcagtgtca gtgagcttct tagcgatttt tgccattttg ggaccctcgg tttttggacc
     1681 cttcttagtg ggtcccattc agggtgccat aactcgtagt tctcagcaat tctcactgga
     1741 cgacaataga cgtaaaaaag cccgcagagc ttgtgctgtg tgggcttagt agacttcatt
     1801 gaacttcaaa caactaaaaa gtggtggagc tggcgggagt tgaacccgcg tccgaaattc
     1861 ctacatcctc ggtactacat gcttagtcag tctttacatt cgcttgccag ctgcggacgg
     1921 acacgccact aacaaactag cctgattaag ttttaacgct tcaaccccag gcagggcttc
     1981 cacgcgatct cttttgggtt tgacctctct tgatccccgt cctaagagcg gaggctaggg
     2041 agagagggct ctaagcaggt tattaagctg ctaaagcgta gttttcgtcg tttgcgacta
     2101 ttttttgcgg ctttttacga ggccaaccgc ccctcggcat gcaccttggg tttcgcaaat
     2161 cccgtcgaat ccagaatcag ccccaatgtg taaaggtaag tataccagat ttatgagcgc
     2221 catgaccagc ctcaatggcg ttatcgttaa agatttagca cccatgtagc ctgattttta
     2281 ttcgattaag caatgggatg gcaacatttg tgtcggatgt gatagccaat aagatgttca
     2341 ttcgcgccgc cggagaggga ggcgcggtga ggaactggtc aataattgga gtgcaggttt
     2401 aacggtgggc gtttttcatg atacgtgctt tatccatctg ccattcgcgt tctttgatat
     2461 ctgaacgttt atcgtgctgt tttttacctt tggcgacgcc gattttcact ttgcaccagg
     2521 catttttcca gtacagggag agcgccacta cggtatagcc ttctcgattg acgcgaccgt
     2581 acaatgagtc cagttcgcgc tggttgagaa gtaacttgcg ggtacgggta ggatcgcaca
     2641 ccacatgcgt ggaggccacg gccattggcg tgatgttagc gccaaacaga aatgcctctc
     2701 cgtcacgcag aaggacgtag ctgtcgctga tattggcttt tcctgcgcgc agggatttaa
     2761 cctcccagcc ttgcagggca agtcccgctt cgaactcttc ttcgataaag tattcgtgac
     2821 gggcgcgctt gttaagcgcg atggtcgctg aaccaggttt atgtgctttt ttcttcgtca
     2881 taagcgtcgt gaatcatcgg taatctgaaa ttgaaaacac cccatatcaa tcctgcgggg
     2941 gacaaggctc tatcttagca tgaacccgat gttacccagc gccgggatag cgtttttttt
     3001 acagcaggat aaatgatatt atttgttgga tttttgttga tggaaattgt tatgcctcag
     3061 attagccgga ccgcactggt accctacagc gcggagcaaa tgtatcagtt agtgaatgac
     3121 gttcagtctt atcctcagtt tttgccgggt tgtaccggaa gtcggattct ggagtccact
     3181 cctgggcaga tgactgcggc ggtagatgtc tctaaggctg ggatcagcaa aacgtttact
     3241 acccgcaacc agttgaccag taaccaaagt attcttatga atctggtgga tgggccgttc
     3301 aagaaattga ttggtggatg gaagtttacg ccgctgagcc aggaggcgtg tcgtatcgag
     3361 tttcatctcg actttgagtt taccaataag ttgattgaac tcgcctttgg tcgcgtgttt
     3421 aaagagctgg cgtctaatat ggtccaggct tttacggttc gtgcgaaaga ggtttacagt
     3481 gccaggtaaa attgccgttg aggtggctta tgcgctacct gagaagcagt acctgcagcg
     3541 cgtgacgctg caggagggcg cgacggttga agaagctatt cgcgccagtg gcttgctgga
     3601 attgcgtacc gatatcgatt taactaaaaa caaagtcggc atttacagcc gtccggcaaa
     3661 actaagcgat agtgtgcatg atggcgatcg ggtggagatt tatcgccctc tcattgccga
     3721 tccgaaagag cttcgcaggc aacgagcaga aaaatcagcg aataaataac agacagaaaa
     3781 aaggtgctca ttgagcacct tttttaacgt ctttgagagc aactttatta ttagttacca
     3841 ctcagcgcag gtttgttatc aatattggtc aacacaccgc tactgttaaa ggtcagcgtc
     3901 agcgtttgct gcgttacacc ttcatgacct ggttgctggc ggaagacata gaaccaggta
     3961 ttcgtaccaa atggatcgga catcagcggt gtacccaatg cgtacgcaac ttgttgttgc
     4021 gtcatgccaa cacgtatttt ggatacgtcg ttagcggtca gatagttccc ctggttgatg
     4081 tcaggacggt aaaccactcg ctccagagtg gaacagcctg cggtcaacat caatagtact
     4141 gctgctgcag cagtcagcgt tttacagcgc atagtgattt gattcctttt cgggcccgag
     4201 cagtacgtgg ctcatctgta atatgccgat gataatagac ctttcatcac tttaaaacct
     4261 tttgctttcc ggtcttacgg cgttttgctg tttactctga ccgtgaagca ggaaaaaagt
     4321 ttacgctgca agcagttctt tcgcattcgc cagtgtatta cgtgtgactt cactgccacc
     4381 aagcaggcgc gccagctctt gtaaccgcgc ttttttattc agggattgca tatgcgtttc
     4441 tgtcatcgca ccatcggttt ctttgctgac aaaatagtgt tgatgaccac atcccgcgac
     4501 ttgtggcagg tgggtaacac acatcacctg agttgattcg ccgagttgac gcagcagttt
     4561 gccgacaact gccgctgttg gaccgctaat ccctacatcc acttcatcaa aaatcagtgc
     4621 cggggtttcc attttacgcg ccgtgatgac ctggattgcc agtgcgatgc gggacaattc
     4681 accaccggat gcgactttgg caataggctg cattggctga cctgggttgg tggttacccg
     4741 aaactcaata cgatcggcac cgtcagcgcc caggtgatgc tcgtcaaatt taacatcgat
     4801 cgtaaactgc ccatgcggca ttgagagcgc atgcatactg tcggtgatca gctgtgcaag
     4861 ttcttctgca tattgctggc gttgttggtg taatgcgcgc gcgatttcca gtgcctgctg
     4921 atgatgtttc gttaccgcca gcgcaagcgt ttcttgtgag tcggcctgat cgtccagttg
     4981 ctgctgttct tccagtagcg actggtaata ctgtggcaat gcctcagggc tgacgtgatg
     5041 tttacgtgcc agcgaaatct gttttgagat gcgctgttca agttcaaata gtcggttggg
     5101 atcgagatcc agacgatcgc agtagtggcg cagttcatcg ctggcttcag caatctggat
     5161 ggtagcttct tccagcatat caagtacgcc ggacagtttg ctgtccatgc caatcaattc
     5221 gctcaccagt tgtttagccg tgtaaagctg actttgcagg tttgcgtctt caccgtcggc
     5281 cattaatgcc aatgcattct ggctggtggt cagcaattga ccgctgttcg ccagacgttt
     5341 gtactcttcg tcgatttgtt caaactctcc gggctgcgga ttaaattcgt taagttcttt
     5401 taattggtat tgcagcagtt ccgcacgggc agcgcgttcc tgacttaact gttgatgatg
     5461 cgcgaggtca cggcagcttt gatgccacaa ctgataacgt gctgtcattt cctggagtag
     5521 agaggtttca ttggcatagc catcaagcag gaacttttgg tgctcgggtt tggtgagtaa
     5581 ctgatgagcg tgctgaccat ggatctgaat cagcaactga cccagttcgc gcagttgtga
     5641 cagaggaaca gctgtaccgt tgatgaaacc acgggagcga ccatcgctgc tgatcacgcg
     5701 acgaagcaaa cattcatgcc cgtcttcaag ctggttttct tccagccagc gcagagccgc
     5761 tggcgtatct ttcagagaaa aacgggcgca caggtcagcg cgagcagcgc cggtacgcac
     5821 catgtcggct tcagcgcgac caccgagaca aagaccgagg gcatctattg caatagattt
     5881 acccgcgccg gtctcgccag ttattacggt catgccgcta tgaaaatcaa tctcaagctc
     5941 acgaacgata gcaaagttgc tgatggtcag ttgtgccaac atagtcgttt tcctgtatga
     6001 aaaaccatta ctgttattgt gtacagtata aactggtttt atatacagta aagaggctgg
     6061 cgtaaaatta gaataatttt tttgaccagc cgagcttggt gcttaatgtg ttgaaataac
     6121 tgtaatcttt cggatgaatc agattcagat ggtaatcaca gcgacgaatc aggacatctt
     6181 caccttcctg aatcggcagt gctatctggc tgtcgcaact gatttccagg tcgttacggc
     6241 gatgcgaaaa acgcagacgg atcgtgctgc tgctgtttat gaccagtggt cgtgctgaca
     6301 acgtatgcgg gaacatgggc accagggtaa tcgcatccag agagggggtc agaataggac
     6361 cgcctgcaga gagggaatag gcggtggagc ctgttggcgt cgaaataatt agtccatcag
     6421 atcgctgaga aaacgcaaag atctcgtcga tatacacttc gaactcaatc atatgcgcca
     6481 ctttgcctgg atgaagcacc acttcattta tcgcggtgct gatgcgtttc tggcaatctt
     6541 gctgacagac ttgcgcttcc agcaaaaaac gtttctcgct gatgtagtgg ccttccagca
     6601 catcggctaa ctgttgctgg gcgttatcgg ggtcaaggtc agtcaagaaa cccaggttgc
     6661 cacggttgat tccaataact ttaatatcgt aacgggcgag tgtgcgcgcc gcgcccagca
     6721 tattaccgtc gccaccaacg actaccgcga gatcagctag ttgcccaatc tccgcgagcg
     6781 tgccagtttt cacattcttc agttgcagtt cgtgagcgat ttgttgctca acgatgacct
     6841 cgtaaccttt tgtgcacagc cagcggtaga gcatttcatg tgttgtcagt gcagtagggt
     6901 gccgtgggtg tcccacaatg ccaatacact tgaaatgatt attcattttt ccgaggtcct
     6961 tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac tgatccccat
     7021 aataagcgaa gttagcgaga tgaatgcgaa aaaaacgcgg agaaattcat gagtagtaaa
     7081 gaacagaaaa cgcctgaggg gcaagccccg gaagaaatta tcatggatca gcacgaagag
     7141 attgaggcag ttgagccaga agcttctgct gagcaggtgg atccgcgcga tgaaaaaatt
     7201 gcgaatctcg aagctcagct ggctgaagcc cagacccgtg aacgtgacgg cattttgcgt
     7261 gtaaaagccg aaatggaaaa cctgcgtcga cgtactgaac tggatattga aaaagcccac
     7321 aaattcgcgc tggagaaatt catcaacgaa ttgctgccgg tgattgatag cctggatcgt
     7381 gcgctggaag tggctgataa agctaacccg gatatgtctg cgatggttga aggcattgag
     7441 ctgacgctga agtcgatgct ggatgttgtg cgtaagtttg gtgttgaagt gatcgccgaa
     7501 actaacgtgc cgctggaccc gaatgtgcat caggctatcg caatggtgga atctgatgac
     7561 gttgcgccag gtaacgtact gggcattatg cagaagggtt atacgctgaa tggtcgtacg
     7621 attcgtgcgg cgatggtaac tgtagcgaaa gcaaaagcgt aatttctgtt ttcgtaataa
     7681 ttcacggccc tgcatgcgaa tgcagggccg ttttcgttac tccgccacac tctcgcgcag
     7741 cggttttaca ggaaagactt ttacctgctt aatcatattg tcctgaacgt cgagaatatc
     7801 gatatcgtac tcgccaatat gcacgcgggt gccagcgaca gggatctctt ccagagcttc
     7861 aagaatgacg ccattaaccg tgcgggcatc gtcttcaggt agatgccagt taaaggcttt
     7921 gttgatttcc cgcacgttgg cagtgccatc aataatcacc gaaccgtcgt tttgcggcgt
     7981 gacctcttcg gcaagtgttg gcgacatcga cgtagtgaaa tcgccgacaa tctcttccag
     8041 aatatcttca accgtcacca gcccctgaat gtctccatac tcgttgacga ccaggccgac
     8101 tttctttttg ttgcgctgaa atttcaccaa ctgcgtgctg agcggcgtac cttccgggac
     8161 aaaatagatc tcgtccgcgg cgcgtagcat ggtttctttg gtgaactctt ttttctccga
     8221 catcaaccgc caggcttcac gcacacgcag catactgatg gcgtcgtcca gcgaatcacg
     8281 gtagagcacg atgcgcccgt gaggtgagtg ggagagttgg cgcagaatcg atttccagtc
     8341 atcgttgata tcaataccga taatttcact gcgcggcacc atgatgtcat caacggtcat
     8401 tttttccaga tcgagcaccg acagcagcat atcctgattg cgacgggaaa tttgtgagcg
     8461 cgattcatgc acgatagtgc gcaactcttc tttgctcaaa gagccgctga ccacgatatc
     8521 ggttttgatg cccatcatgc gcattaacat gcgggtgata gcattcagca accagaccag
     8581 cggcatcatc aaaatttgca gcggagccag cagaaaacta ctcggataag cgactttttc
     8641 cgggtacagc gcggcaatgg ttttcggcaa tacctcagca aataccagta cgacaaaagt
     8701 cagcacacca gtcgcaattg ccacgcccgc atcgccgtac aaacgcatcc caacaatagt
     8761 gccgagcgcg gaggcaagaa tattgaccag gttattgccg attaacacca ggcttatcag
     8821 gcggtctggc ttacgcagca atttttcgac gcgtttggcc gagcgattac cctgtttcgc
     8881 catatgtcgc agacgatagc ggttgagggt catcattccg gtttcggacc cggaaaaata
     8941 ggctgaaatg accaccatga tgatcagaat aatgatcaac gtagtagtag aaatgtgttc
     9001 caggggaaac tccttttctg ggtttagctg attaactgct ggacaattcg gctgccgaag
     9061 taggccagtg tcagaattac cgcgcccgca acgttaaacc agacgacgcg gcgtccacgc
     9121 catccttcat ggtaatgtcc ccacagcagc acaatataga caaaccacgc cacgatagag
     9181 agcacagcct tgtcgatatt ttccatgcta aataggttgt gcatgtagaa caggccagtg
     9241 caaagcgtga gcgttagcag caccacgcca atctgcgtga tgtggaacat tttacgctcg
     9301 atactcatca atggcggcat ttcctggtta aatgccagct tcttgttctt cagttggtaa
     9361 tcaatccacg ccagttgcag cgcgtacagg gcggcgataa ttagcgtggc ataggaaaag
     9421 agcgataagc caatgtgcac cagcatccca ggcgttgctt ccagatgggt gatgtattca
     9481 ttgggcatga aggtcgccag cgccaggttg ataagcgcaa aggcatagac aatgggtagc
     9541 agcaaccagc cacgattgcg agaagccaca atggtcatta ccgtacagat catcaaactg
     9601 accaatgaac caacgttcag caggctgagg ttttgtccgc tatcaccgtc gggcaggatg
     9661 cgggcttcca gagcgattgc gtggcagacc agcgcaatga ccgcagaaat aatagccatg
     9721 cgccgccagc cgccgttttt ttgcagcaga ccgggaacaa tcagcgcaag actgacggag
     9781 taggcgacaa gcgcgagcag agcaaaaacg ggcatagtga tgtcgacagt ttgagcaata
     9841 agaaagagaa gcagtatagc gtcaggtgaa cgctgctcca accgttgcat aacaacaaag
     9901 acgccttcat gttatactgc ggcaaaatac tgatgatgtg tcgcgattgc ggccaaccgt
     9961 ttccacccca ggcgagagac aatgtttgat aatttaaccg atcgtttgtc gcgcacgctg
    10021 cgcaatatca gtggccgtgg acgcctcact gaagacaacg taaaagatac gctgcgcgaa
    10081 gtgcgcatgg cgctgctgga ggcggacgtc gctctgccgg tagtgcgtga gtttatcaat
    10141 cgcgtaaaag agaaagcggt tggtcatgaa gttaataaga gcctgacgcc ggggcaggag
    10201 ttcgtcaaaa tcgtccgtaa cgaactggtt gcggcgatgg gcgaagagaa ccagaccctg
    10261 aacctggcgg cgcaaccgcc tgcggtcgtg ctgatggcgg gcctgcaagg tgccggtaaa
    10321 acaaccagcg ttggtaaact cggtaagttc ctgcgcgaga agcacaagaa gaaagtgctg
    10381 gtggtttctg ccgacgttta tcgcccggcg gcaatcaaac agcttgagac gctggcagag
    10441 caggtgggcg ttgatttctt cccttctgat gttggtcaga agccggtaga tatcgttaac
    10501 gcggcgctga aagaagccaa actgaaattc tacgacgtgc tgctggtgga taccgctggt
    10561 cgtctgcacg ttgacgaagc gatgatggac gagatcaaac aagtccatgc gtcgattaac
    10621 ccggttgaaa ccctgtttgt ggttgacgcc atgaccggtc aggatgcggc caatacggca
    10681 aaagcattca atgaagcgtt accgcttacc ggcgtagtgt tgaccaaagt ggacggcgat
    10741 gcccgcggcg gtgcggcgct ctctattcgt cacatcactg gcaaaccgat caagtttctc
    10801 ggtgttggcg agaaaactga ggcgctggag ccgttccatc cggaccgcat cgcgtcgcgt
    10861 attctcggca tgggcgacgt actgtcgctg atcgaagata tcgaaagcaa agttgaccgc
    10921 gcgcaggcag agaaattagc cagcaagctg aaaaaaggcg acggcttcga tctcaacgat
    10981 tttcttgagc aactgcgcca gatgaaaaat atgggcggca tggctagcct gatgggcaag
    11041 ctgccgggca tggggcagat cccggataat gttaagtcgc agatggacga taaagtgctg
    11101 gtgcgtatgg aagccatcat caactcgatg acgatgaaag agcgcgctaa gccagaaatc
    11161 atcaaaggtt cgcgtaaacg ccgtattgct gccggttgcg gtatgcaggt gcaggacgtt
    11221 aaccgtcttc tgaaacagtt cgacgacatg cagcgcatga tgaagaaaat gaagaagggc
    11281 ggaatggcga agatgatgag aagcatgaag gggatgatgc cgccgggatt tcctggtcgc
    11341 taaatgacct tatgcgacag agttattaac tgctgattgc attttctcca gaaatcagta
    11401 aaattttcgg gcttttaata tgacaccgga ctccgttcct cgatggggtc cggttgtttt
    11461 attcacacaa gaggatgtta tggtaactat tcgtttagca cgtcacggcg ctaaaaagcg
    11521 tccgttctac caggttgttg tcgctgacag ccgtaatgca cgcaacggtc gcttcatcga
    11581 gcgcgttggt ttcttcaacc caatcgctag cgaaaaagaa gaaggcactc gcctggatct
    11641 ggatcgcatc gctcactggg ttggccaggg cgcaactatt tctgatcgcg ttgctgcgct
    11701 gatcaaagaa gtaaacaaag cagcttaatc tgtcacggtg gtcatgatga gcaaacaact
    11761 caccgcgcaa gcacctgttg atcccatcgt tttgggaaaa atgggttcgt cttacggtat
    11821 tcgtgggtgg ctcagagtgt tttcttccac cgaagacgcc gaaagcattt ttgactatca
    11881 gccctggttt atccagaagg cgggtcagtg gcagcaagtc cagctggaaa gctggaagca
    11941 ccacaatcag gacatgatca tcaagctgaa aggcgttgac gatcgtgatg cggcgaacct
    12001 gctgacgaat tgtgaaattg tcgtggattc atcgcagctg cctcagcttg aagagggtga
    12061 ctactactgg aaagacctga tgggctgcca ggtagtaacc actgaaggct acgatctcgg
    12121 taaagtcgtc gatatgatgg aaaccggatc taatgacgtt ctcgtcatta aggcaaacct
    12181 gaaagatgcg tttggtatca aggaacgtct cgtaccgttc ctcgatgggc aggttatcaa
    12241 gaaagtcgat ctcactactc gttcaatcga agtagattgg gatcctggtt tttaaaccac
    12301 cggataaacg gtaaaagacg gcgctatgtg gattggcata attagcctgt ttcctgaaat
    12361 gttccgcgca attaccgatt acggggtaac tggccgggca gttaaaaatg gcctgctgag
    12421 catccagagt tggagtcctc gcgacttcac gcatgaccgg caccgtaccg tggacgatcg
    12481 tccttacggc ggcggaccgg ggatgttaat gatggtgcaa cccttgcggg acgccattca
    12541 tgcagcaaaa gccgcggcgg gtgaaggcgc aaaggtgatt tatctgtcac cacagggacg
    12601 caagcttgat caagcgggcg tcagcgaact ggcaacgaat caaaaattga ttctggtgtg
    12661 cggtcgctac gaaggtatag atgagcgcgt gatccaaacc gaaattgacg aagaatggtc
    12721 aatcggcgat tacgttctca gtggtggtga gttaccagca atgacgctga ttgactccgt
    12781 ttcccggttt attccgggag tactgggaca tgaagcctcg gcaacggaag attcctttgc
    12841 tgaaggattg ctggattgcc cgcactatac gcggcctgag gtgttagaag ggatggaagt
    12901 tccgccagtg ttactgtcgg gcaaccatgc cgagatacgt cgctggcgtt tgaaacagtc
    12961 gctgggccgt acctggctta gaagacctga acttctggaa aacctggctc tgactgaaga
    13021 gcaagcaagg ttgctggcgg agttcaaaac ggaacacgca caacagcaac ataaacatga
    13081 tgggatggcg taagcccccg agatatcagt ttacccagga taagagatta aattatgagc
    13141 aacattatta agcaacttga acaagagcag atgaagcagg acgtaccttc cttccgtccg
    13201 ggtgataccg tggaagtgaa agtatgggtt gttgaaggtt ccaaaaaacg tctgcaggca
    13261 ttcgagggcg tggttatcgc tattcgtaac cgcggtctgc actctgcatt cactgttcgt
    13321 aaaatttcca acggcgaagg cgttgagcgt gtcttccaga ctcactctcc ggtagttgac
    13381 agcatttctg tcaaacgtcg tggtgctgtt cgtaaagcta aactgtacta cctgcgtgag
    13441 cgtactggta aggctgctcg tatcaaagag cgtcttaact aagatttcgc ttaagcgaca
    13501 tcctgttaag aagggctggc caattggctg gccctttttt tgtctgtttg ctcgtgtttt
    13561 aaggggttgt aatcaccact gcgacgcggc ggttctcggc gcggccctgg gcggtcttgt
    13621 tactggctat gggatatttt tttcctaaac cctgtgtggt gagattgctg cgtggaattt
    13681 gtccacccat agcccatgca tcggccacga cattcgcccg tttcaatgat aagccttcgt
    13741 tgtaactgtc ttcaccatag ttatcggtgt gtccatccat acgggcatgt gttagccctg
    13801 tcgaggccaa tttggctgcc atggtttgga tctgttgctg gctttccggg agcaatttgt
    13861 agtcattttt tgcgaacaga atggcatctg ataagcccag cgaccagtcg ccggcggatt
    13921 cagtaaatcc ataagattgc atagcggcga cttgctcagg agtaaacttt ccctgagggg
    13981 actggcagcc agtcagtatt agtgaggtga aaatcagggg tgctaccagg tgctttatca
    14041 tatcgatata tccttgttat ctcaccagct tttcggcacg ctggtgtttg gcctgataca
    14101 tattgtgatc ggcaagctct tgtaattttt ccgcagaggc gtgctcaatg gtcatcgcgt
    14161 aaccaatgct taatgtcatg gtggtctgat ggccattatg aagatcaaac gggagattaa
    14221 agatttgtgt cagtgctgag catatctgct gcacttcaga ttccgactgt acatcataga
    14281 gcaccatagc gaattcatcg ccgcccaggc ggtatgcttt atgtcgcagc ccgccaaatt
    14341 cagctaaccg ttttgcgatt tcaatcaaga ctctatcgcc cgtcgcatga ccccaggtat
    14401 cattgatata tttgaaatta tcgccatcaa gaaatagtaa cgccgacgtt tttcgggcat
    14461 cggaattgtt catcaacgta ttgatgccgc tacgaaacgc tgcgcggtta gccagcccgg
    14521 ttaatgggtc atgtagcgcg gtacgcagaa gctgcgcatt tttggcctgt aaacgaagct
    14581 gccactcttc catttcatcc agcagactgt tgaagtcgag agcgaagcgg tgaaactcag
    14641 cgatacgttc ttccgaaact cgtcgggaaa aattgcggtt ggaacgcaca tcatgtacga
    14701 catcggtgat atttttcagc gcttctacca ggccattgtg caaatggcgg gtgagggtaa
    14761 ttgcgatgcc tgatgccagc agaatacaac cggtcagtac ggcgagcgaa aaccagataa
    14821 aatggctgat tgaactgtcg cgagcggtta agcgtacttc gccaatggtt tcaccattgt
    14881 gacgaatcgg ctgaatgatg ggggcgggga agagccagtg gcttatgaaa ttgctgaaag
    14941 tatcgcctgg atccttacgg gtgtaatgcc aggacgccag aatattttgc tgcttatcac
    15001 gtacttctgc agttgaaaat tgcccttgct ggcccagcgc tgccagtgtt tcagttgctg
    15061 cagggccatc ggcaaaaacg acagctgctt ccagactgta agtcattgtt gctgctgtca
    15121 gtgccaggtt tttttgcgcg tactgtttca gggtcagcac cgaggtcacg gaaagcagca
    15181 accagatcag catcatagtg ataaatatgc tggtcatact gatgttgcgt aatgctcttt
    15241 taaacgtggg gcgcttatta agagaattat cgttatccat catttttctt ccgtgcgagc
    15301 attaagacat ccgggttgac ctttacaccg ctacgcgata aggcatccag gtttacggca
    15361 aatctgacgt cattattgtg gatgatcaga caaaaggcgc taccaataat gcactcggta
    15421 ttttgttcgg caattaataa taacgccttt gacggatatt gttcggttaa ttccatctgg
    15481 aatgtcggtg actcgttgcc aaaataaaaa ccattgcagc ctgaaatcat cgcctcttgt
    15541 tgagtgtgaa tgatgacagg cagataaggc aaagatgtcg cggcattctc ttgtagggcg
    15601 gtactaaaac gtgaagatga aaagatacat aactttggtg gccctgatag cgcaggccag
    15661 cgggtgtaac tgacaatacc ggaaaccatc atacgcacgt ttttcgcaac gtcagaaggc
    15721 tcctgagcga gtattggtgc tcccgtcagc aggagaatca gaagtaagaa cagtcggtga
    15781 gaaaaacgca ttcagggatc cccaccatct tcgctcgaaa tgcatcgata ttttttcaga
    15841 atgctcatta aaatatcatt ttttcatgcg atcgtcatca atgtgagaaa taaaacacaa
    15901 tgagtaaaat cttatgttta atttatttgt ggggttttaa ttaatttaat cgtattgtgc
    15961 tggcattttc atcagcccct ttttgccata cttcatgcag ttggctggca ttttcaacat
    16021 tgttgcagct ggcaggaaag cttttaccgg aaagtccccg ggcataagta aaatcggtct
    16081 ggcaagtttt cttttgtcct tcggcatatc ctttaaggta tagaccgcga tccgcctgtg
    16141 aatcgctaaa tgcatcgtca tcttttatgg cgctgcccga tatcgcatct tccataccaa
    16201 catcatacca gtcggtgctg gtcaaggttg gcgcgtgagt ataaggatca atctgacatc
    16261 cgctaaccag taaagccaac aagggggcga taaacttttt catcatttct ttctcctttt
    16321 tcaaagcata gcggattgtt ttcaaaggaa gtgtaaattt atctatacag aagtaagggt
    16381 tgaaattgcg actaaattgc ctgtgtaaat aaaaatgtac gaaatatgga ttgaaaactt
    16441 tactttatgt gttatcgtta cgtcatcctc gctgaggatc aactatcgca aacgagcata
    16501 aacaggatcg ccatcatgca aaaagacgcg ctgaataacg tacatattac cgacgaacag
    16561 gttttaatga ctccggaaca actgaaggcc gcttttccat tgagcctgca acaagaagcc
    16621 cagattgctg actcgcgtaa aaccatttca gatattatcg ccgggcgcga tcctcgtctg
    16681 ctggtagtat gtggtccttg ttccattcat gatccggaaa ctgctctgga atatgctcgt
    16741 cgatttaaag cccttgccgc agaggtcagc gatagcctct atctggtaat gcgcgtctat
    16801 tttgaaaaac cccgtaccac tgtcggctgg aaagggttaa ttaacgatcc ccatatggat
    16861 ggctcttttg atgtagaagc cgggctgcag atcgcgcgta aattgctgct tgagctggtg
    16921 aatatgggac tgccactggc gacggaagcg ttagatccga atagcccgca atacctgggc
    16981 gatctgttta gctggtcagc aattggtgct cgtacaacgg aatcgcaaac tcaccgtgaa
    17041 atggcctccg ggctttccat gccggttggt tttaaaaacg gcaccgacgg cagtctggca
    17101 acagcaatta acgctatgcg cgccgccgcc cagccgcacc gttttgttgg cattaaccag
    17161 gcagggcagg ttgcgttgct acaaactcag gggaatccgg acgggcatgt catcctgcgc
    17221 ggtggtaaag cgccgaacta tagccctgcg gatgttgcgc aatgtgaaaa agagatggaa
    17281 caggcgggac tgcgcccgtc tctgatggta gattgcagcc acggtaattc caataaagat
    17341 tatcgccgtc agcctgcggt ggcagaatcc gtggttgctc aaatcaaaga tggcaatcgc
    17401 tcaattattg gtctgatgat cgaaagtaat atccacgagg gcaatcagtc ttccgagcaa
    17461 ccgcgcagtg aaatgaaata cggtgtatcc gtaaccgatg cctgcattag ctgggaaatg
    17521 accgatgcct tgctgcgtga aattcatcag gatctgaacg ggctgctgac ggctcgcgtg
    17581 gcttaagagg tttattatgg ttgctgaatt gaccgcatta cgcgatcaaa ttgatgaagt
    17641 cgataaagcg ctgctgaatt tattagctaa gcgtctggaa ctggttgctg aagtgggcga
    17701 ggtgaaaagc cgctttggac tgcctattta tgttccggag cgcgaggcat ctatgttggc
    17761 ctcgcgtcgt gcagaggcgg aagctctggg tgtaccgcca gatctgattg aggatgtttt
    17821 gcgtcgggtg atgcgtgaat cttactccag tgaaaacgac aaaggattta aaacactttg
    17881 tccgtcactg cgtccggtgg ttatcgtcgg cggtggcggt cagatgggac gcctgttcga
    17941 gaagatgctg accctctcgg gttatcaggt gcggattctg gagcaacatg actgggatcg
    18001 agcggctgat attgttgccg atgccggaat ggtgattgtt agtgtgccaa tccacgttac
    18061 tgagcaagtt atcggcaaat taccgccttt accgaaagat tgtattctgg ttgatctggc
    18121 atcagtgaaa aatggaccat tacaggccat gctggcggcg cacgatggcc cggtactggg
    18181 gttacacccg atgttcggcc cggacagcgg tagcctggca aagcaagttg tggtctggtg
    18241 tgatggacgt aagccggaag cataccaatg gtttctggag caaattcagg tctggggcgc
    18301 tcggctgcat cgtattagcg ctgtcgagca cgatcagaat atggcgttta ttcaggctct
    18361 gcgccacttt gctacttttg cttatgggct gcatctggca gaagaaaatg ttcagcttga
    18421 gcaacttctg gcgctctctt cgccgattta ccgccttgag ctggcgatgg tcgggcgact
    18481 gtttgctcag gatccgcagc tttatgccga cattattatg tcgtcagagc gtaatctggc
    18541 gttaatcaaa cgttactata agcgtttcgg cgaggcgatt gagttgctgg agcagggcga
    18601 taagcaggcg tttattgaca gtttccgcaa ggtggagcac tggttcggcg attacgcaca
    18661 gcgttttcag agtgaaagcc gcgtgttatt gcgtcaggcg aatgacaacc gccagtaata
    18721 atccagtgcc ggatgattca catcatccgg caccttttca tcaggttgga tcaacaggca
    18781 ctacgttctc acttgggtaa cagcccaata ccttcattga acgggtaatt tcccctaact
    18841 ctttcaatgc tttttgcatt tccgctgatt caagattggc ctgaatatcc agataaaaca
    18901 tctcttccca tggattaccg tgaatcgggc gtgattccag acgggtcata atcagattgt
    18961 ggttgcgcag caccagcaac gcttcaacca gcgcaccggc ttgttgcccg gtcgccatta
    19021 acaacgtcgt tttcgccgga acctggtcag acacgttaat ggctttacgt gccaacacca
    19081 caaatcgggt gaagttttgt tgctgattcg cttcaatacg ctccagtacc tgcaaaccgt
    19141 acaaagtgcc gccagcttcg cttcccaatg cagcaacatg cggtgatttt gcctgtgcaa
    19201 ccttttccat tgccgcagac gtactttcgg tatattcaat cttccagtga ggataacgat
    19261 taaggaattt gctgcattgc tggaatggct gcggatggct gtagaccgta ttaatggtgg
    19321 ataaatcagt cgtgccggag accaacaaac aatggtcgat agttaacgtc atctcgccaa
    19381 caatcgacaa gctggtatgt tgcagcagat cgtaaacgtc gtttatggca ccggagctgg
    19441 tattttcaat cggtacgacg gcatagtcgg cctggccggt ttccacctga ttaaaaatat
    19501 cggcaaattt ggcgcagcca ctttcaatga attgctcaaa gtgacgggca gcatactggc
    19561 gcgccgcaag atgggaataa gaacctttgg ggccgagaaa agcgatgcgt gctgagtgcg
    19621 gattaatttt attgagatgt tgctggagca aagcctgctg agttaatacg gaatcttcaa
    19681 tgatgagctg gaacaggcga gtaatgtaat gggcgtccag atggtgcgct ttaccgagcg
    19741 taattaatct ttccagtaaa tcgcgttcac gatcaatatc tcgtaccggg cgatgcgaga
    19801 gcagtttggc ttttcctacc tcgacggcca attcgcgccg ctctgccagt aatgctaata
    19861 atttttcatc cagcgcgctg attttctctc gcagcgccag taacgggttt tccgatgtca
    19921 tagtgttgcc tttttgttat caataaaaaa ggccccccga tttgggaggc cttattgttc
    19981 gtcttcgcat tctgtttcac acgacgaaac gcctcccatt caggggaagg taaaaaagaa
    20041 tgcgaagaaa aacggtgtgt gtttcatgtt tgtttcctta agtgacttta gtacagtacc
    20101 cgtactgttt tcacgctgtc aacaaaaaac gcgcccgaag gcgcgttggc gatacactca
    20161 atataaagga ctactcttct tcaacttctt cgacgaagtt ggcgtctttc accgatgttg
    20221 cggcacgacg tgcttcgcct ttgtgctgca gtttattgag ctgccgttcc agcttgttga
    20281 tcaattcgtt aattgcagtg tacatatctt catgtttacc actggcaacc agaacgccgt
    20341 taggtgtatt gattgtggcg tcagcaacaa acccttgtgg ctctttggac agaatgatat
    20401 gtggattaat cagatgtgtt tgccattttt ccagtttggc gagacggtct gcgacatgtt
    20461 ggcggatggc cggagtaatt tccatttgtt tgctggtaat gttcattgtc ataaatttta
    20521 cctcttgtct tcccgtcttg gtgaattcag cataccgctc ctgatgtcaa aatgtgtgat
    20581 gggaatctca taattttgtc actttttgtc aacgaatctt ttttgtgaga aatcgcagga
    20641 agaggtgttt ttttacttga ggaatatctc aaagcgagcc atatttgatg agatcgatag
    20701 tgactaaatc gcttcagttt cacaactaac agaataaaaa aacggcagct caagggctgc
    20761 cgttttgtat ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
    20821 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
    20881 catcacgcgt agcctgggta tccgggtagt cgcgcaacat tccttctacg cggttaacga
    20941 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
    21001 gacgatcttt caggaatacc agacgtttgg tggcatcggt ggtgtactga ctgtttggat
    21061 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
    21121 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
    21181 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
    21241 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
    21301 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
    21361 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
    21421 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
    21481 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
    21541 aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac aatagcacac
    21601 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
    21661 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
    21721 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
    21781 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
    21841 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
    21901 ccgctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgacctg
    21961 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
    22021 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
    22081 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
    22141 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
    22201 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
    22261 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
    22321 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
    22381 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
    22441 gcttcggaag catttatctc catgctgcgt aagtttgacc gccaggcgct tcatgcaacc
    22501 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
    22561 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
    22621 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctaccaaaa ggtgttgcgg
    22681 cctgtagctc ttctcgtatc ggcggcgtga gcttgccccc gtatgactcg ctcaacctcg
    22741 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
    22801 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
    22861 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcccg
    22921 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
    22981 gaacggaagt cgccgccgct catgctggct ggcgtggact gtgcgcaggc gtgctggaag
    23041 agacggtttc ctgttttgct gataatccgg aaaatattct cgcctggtta gggccggcaa
    23101 ttggtccacg cgcgttcgaa gtgggggtgg aggttcgcga ggcgtttatg gcagtagacg
    23161 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
    23221 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
    23281 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
    23341 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
    23401 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
    23461 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
    23521 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caatttatcg
    23581 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
    23641 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
    23701 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
    23761 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
    23821 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcagcag
    23881 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
    23941 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
    24001 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
    24061 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
    24121 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
    24181 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
    24241 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
    24301 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
    24361 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
    24421 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
    24481 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
    24541 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
    24601 ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
    24661 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
    24721 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
    24781 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
    24841 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
    24901 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
    24961 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
    25021 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
    25081 aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
    25141 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
    25201 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
    25261 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
    25321 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
    25381 tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg gttggtgcgc
    25441 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
    25501 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaacattc
    25561 tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg gtcgacttcc
    25621 gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag gaacgcttcg
    25681 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc cataacttcc
    25741 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
    25801 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
    25861 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggttacgatc
    25921 cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa aacccgctgg
    25981 cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg gaagttaatg
    26041 aagaccggat tgtcgccgtc cagtaaatga taaaacgagc ccttcggggc tcgtttttgt
    26101 ctataagtta gacggaaaag actatattta agatgttttg cctgaaaaat gagcgaacga
    26161 taaagttttt atatttttcg cttgtcaggc cggaataact ccctataatg cgccacgact
    26221 gacacggaac aacggcaaac acgccgccgg gtcagcgggg ttctcctgag aattccg
//
LOCUS       DAMZII010000068        25766 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZII010000068 DAMZII010000000
VERSION     DAMZII010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.068"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2203
                     /gene="crfC"
                     /locus_tag="POA77_002686"
     CDS             <1..2203
                     /gene="crfC"
                     /locus_tag="POA77_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDI6366087.1"
                     /translation="SQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNIAE
                     RHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLIRH
                     TPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENGVA
                     FEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGLES
                     YPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAILA
                     VGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLANR
                     ARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLFAQ
                     PIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQVE
                     ESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEPLSEIR
                     TCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPGFH
                     FPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSP
                     TETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTRL
                     RESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            2200..3078
                     /locus_tag="POA77_002687"
     CDS             2200..3078
                     /locus_tag="POA77_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDI6366088.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            3342..4844
                     /gene="proP"
                     /locus_tag="POA77_002688"
     CDS             3342..4844
                     /gene="proP"
                     /locus_tag="POA77_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDI6366089.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4956..5045
                     /gene="pmrR"
                     /locus_tag="POA77_002689"
     CDS             4956..5045
                     /gene="pmrR"
                     /locus_tag="POA77_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDI6366090.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5021..6121)
                     /gene="pmrB"
                     /locus_tag="POA77_002690"
     CDS             complement(5021..6121)
                     /gene="pmrB"
                     /locus_tag="POA77_002690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDI6366091.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6122..6790)
                     /gene="basR"
                     /locus_tag="POA77_002691"
     CDS             complement(6122..6790)
                     /gene="basR"
                     /locus_tag="POA77_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDI6366092.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6787..8430)
                     /gene="eptA"
                     /locus_tag="POA77_002692"
     CDS             complement(6787..8430)
                     /gene="eptA"
                     /locus_tag="POA77_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDI6366093.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8534..9871)
                     /gene="adiC"
                     /locus_tag="POA77_002693"
     CDS             complement(8534..9871)
                     /gene="adiC"
                     /locus_tag="POA77_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDI6366094.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10008..10769)
                     /gene="adiY"
                     /locus_tag="POA77_002694"
     CDS             complement(10008..10769)
                     /gene="adiY"
                     /locus_tag="POA77_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDI6366095.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11094..13361)
                     /gene="adiA"
                     /locus_tag="POA77_002695"
     CDS             complement(11094..13361)
                     /gene="adiA"
                     /locus_tag="POA77_002695"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDI6366096.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13560..14468)
                     /gene="melR"
                     /locus_tag="POA77_002696"
     CDS             complement(13560..14468)
                     /gene="melR"
                     /locus_tag="POA77_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDI6366097.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14751..16106
                     /gene="melA"
                     /locus_tag="POA77_002697"
     CDS             14751..16106
                     /gene="melA"
                     /locus_tag="POA77_002697"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDI6366098.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16209..17630
                     /gene="melB"
                     /locus_tag="POA77_002698"
     CDS             16209..17630
                     /gene="melB"
                     /locus_tag="POA77_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDI6366099.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17769..18398)
                     /locus_tag="POA77_002699"
     CDS             complement(17769..18398)
                     /locus_tag="POA77_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDI6366100.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(18521..20167)
                     /gene="fumB"
                     /locus_tag="POA77_002700"
     CDS             complement(18521..20167)
                     /gene="fumB"
                     /locus_tag="POA77_002700"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI6366101.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20245..21585)
                     /gene="dcuB"
                     /locus_tag="POA77_002701"
     CDS             complement(20245..21585)
                     /gene="dcuB"
                     /locus_tag="POA77_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDI6366102.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22156..22875)
                     /gene="dcuR"
                     /locus_tag="POA77_002702"
     CDS             complement(22156..22875)
                     /gene="dcuR"
                     /locus_tag="POA77_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDI6366103.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(22872..24503)
                     /gene="dcuS"
                     /locus_tag="POA77_002703"
     CDS             complement(22872..24503)
                     /gene="dcuS"
                     /locus_tag="POA77_002703"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDI6366104.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24684..24914
                     /locus_tag="POA77_002704"
     CDS             24684..24914
                     /locus_tag="POA77_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDI6366105.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            24926..25198
                     /locus_tag="POA77_002705"
     CDS             24926..25198
                     /locus_tag="POA77_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6366106.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFSKHEFDKTREYDDIRS"
     gene            25425..25721
                     /gene="ghoS"
                     /locus_tag="POA77_002706"
     CDS             25425..25721
                     /gene="ghoS"
                     /locus_tag="POA77_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI6366107.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT         6248 a         6215 c         6689 g         6614 t
ORIGIN      
        1 aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt tactggaaaa
       61 aatgcctctc tctgtacccg gagacgacgc gccacaactg gctttaccct ggagtcagcc
      121 taatatcgcc gaacgtcacg cgatgctgaa taatgagttg cgtaaaattt cccgactgga
      181 aatggtgctg gcaattgtcg gtaccatgaa agcagggaaa tcaaccacca ttaatgccat
      241 tgttggtacg gaggttctgc ctaatcgtaa tcgcccaatg actgcgctgc cgacgcttat
      301 tcgccatacg cccgggcaaa aggaaccggt attgcatttt tcacatgtcg cgccaatcga
      361 ttgtttaatt caacaattac aacagcgcct gcgtgattgc gatattaagc atctgaccga
      421 tgtgctggaa atagataaag atatgcgtgc gcttatgcag cggatcgaaa atggcgtcgc
      481 tttcgaaaaa tattatctgg gtgcccagcc tatttttcat tgtctgaaaa gtttgaatga
      541 tttagtgcga ctggcgaagg cgctggacgt cgattttcct ttttctgctt acgccgccat
      601 tgagcatatt cccgtgattg aagtggagtt tgtccatctg gcggggctgg agagttatcc
      661 cggtcagttg acgttactgg atacccccgg gccaaatgaa gccgggcaac cgcatctgca
      721 aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta ctggcggtgc tggattatac
      781 gcaactgaaa tcgatctccg atgaagaggt ccgtgaggcg attttggcgg tggggcaatc
      841 ggtgccgctg tatgtgctgg tcaataagtt cgatcaacag gatcgtaaca gtgacgacgc
      901 cgaccaggtg cgggcactga tttccgggac gctgatgaaa ggctgtatta cgccacagca
      961 gatatttccg gtgtcgtcga tgtggggcta cctggcgaat cgggcgcgct atgagttagc
     1021 caacaacggt aagttaccag cgccagagca acaacgctgg gtggaagatt ttgcccatgc
     1081 cgcgctcggc aggcgctggc gtcatgccga tctggcggac ctcgaacata ttcgtcatgc
     1141 tgccgatcag ttgtgggaag attcgctgtt cgcccagcca attcaggcgt tgcttcatgc
     1201 cgcttacgct aacgcctcgt tgtatgcttt gcgatctgct gcgcataaac tgttgaatta
     1261 cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac gggttaaacg tcgcttgtga
     1321 acaattgcgg caaaatatcc atcaggtcga agaaagtttg cagctattgc aactcaatca
     1381 ggcgcaggtg agcggcgagg ttaaacatga aatcgagctg gccctgacct ccgccaacca
     1441 ctttctgcgt caacagcaag atgcgctgaa tgcgcagtta gccgccttgt ttcaggatga
     1501 ttcggagccg ttaagcgaga ttcgtacctg ctgtgagaca ctgttacaga cggcgcagaa
     1561 caccatcagt cgcgacttta cgctgcgttt tgccgagctt gaatccaccc tttgccgggt
     1621 gttaaccgat gttattcggc ccattgagca acaagtcaaa atggaattga gcgagtcagg
     1681 gtttcgtcct gggtttcatt ttcctgtttt tcacggcgta gttccccact tcaacactcg
     1741 ccagctgttc agtgaagtca tttcgcgcca ggatgcaacg gacgagcaga gcacgcgttt
     1801 aggcgttgtg cgtgagactt tttcgcgctg gttgaatcag cccgactggg gacggggaaa
     1861 tgagaaatcc ccgacagaaa cggttgatta cagtgtgttg caacgagctt taagcgcaga
     1921 agtcgatctt tattgccaac aaatggctaa agttctggca gagcaggtcg atgaatctgt
     1981 tacggcaggc atgaatactt ttttcgctga gttcgcttca tgtttgacgg aattacagac
     2041 gcgtttacgc gaaagtctgg ctctgcgtca acaaaatgaa tcggtggtca ggctgatgca
     2101 gcagcaattg cagcagactg tgatgactca cggctggatt tacaccgacg cccagctgtt
     2161 acgcgatgat attcaaacac ttttcacggc agaacgatat tgaccaagac gttacttgac
     2221 ggccccggtc gcgtgctgga gtcggtttat ccccgctttt tagtggatct ggcgcagggc
     2281 gatgatgccc gccttccaca agcccatcag cagcagtttc gtgaacgact gatgcaggaa
     2341 cttcttgcgc gcgtgcagct tcagacatgg acgaacggcg gcatgttaaa tgcgccgctt
     2401 agcctgcgac tgacaatggt ggaaaaactg gcgtcgatgc tggatcccgg tcatctggcg
     2461 ctgacgcaga tcgcgcagca tctggcgctg ctgcaaaaaa tggatcaccg ccagcactct
     2521 gctttcccgg agctccccca gcaaattgcc gccttgtatg agtggttttc agcccgttgc
     2581 cgctggaagg aaaaggcgtt aacgcaacga ggcctactgg tgcaggcagg tgatcagagc
     2641 gagcaaattt ttacccgctg gcgtgctggg gcgtataacg cctggtcgtt gcctgggcgc
     2701 tgttttatcg ttctcgaaga gttgcgttgg ggggcatttg gcgatgcctg tcgtctggga
     2761 agcccgcaag cggtggcgtt gttgctgggt gatttgcgcg tgaaagcgac acaacatctg
     2821 gcagagagta tcaatgcggc cccgaccacg cgtcactatt accatcagtg gtttgcctct
     2881 tcgaccgttc cgacgggcgg ggatcatgct gattttttaa gttggctggg aaagtggacc
     2941 acggcagata aacaacccgt ttgctggtca gtgacccaac gctggcaaac tgtcgcgctg
     3001 gggatgccac gactctgttc agcgcagcgt ctggcggggg caatggtcga ggaaatcttc
     3061 tctgtaaatt tggcgtaaat aatcagttac atcaatgagt cctaaacgaa atccatgtgt
     3121 gaagttgatc acaaatttaa acactggtag ggtaaaaagg tcattaactg cccaattcag
     3181 gcgtcaactg gtttgattgt acattcctta accggagggt gtaagcaaac ccgctacgct
     3241 tgttacagag attgcatcct gcaattcccg ctcccctttt gcggccgtcg cgctgatttt
     3301 tctggcgttt gcggaaatgg gccaactctg cgaggaaagc tatgctgaaa aggaaaaaag
     3361 taaaaccgat tacccttcgt gatgtcacca ttattgatga cggtaaactg cgtaaagcca
     3421 ttaccgcagc atcactgggt aatgcaatgg aatggtttga ttttggtgtt tatggttttg
     3481 ttgcttacgc attaggtaaa gtttttttcc cgggggctga ccccagcgtg cagatggttg
     3541 ctgcacttgc cacgttctcc gttccctttc tgattcgacc gcttggcggg ctcttctttg
     3601 gtatgttggg cgataaatat ggtcgccaga agatcctcgc tatcactatt gtgattatgt
     3661 cgatcagtac gttctgtatt ggcttaatac cgtcctacga cacgattggt atttgggcac
     3721 cgattctgct gttgatctgt aagatggcac aaggtttctc ggtcggcggt gaatataccg
     3781 gggcgtcgat atttgttgcg gaatactcac ctgaccgtaa acgtggcttt atgggcagct
     3841 ggctggactt cggttctatt gccgggtttg tgctgggtgc tggcgtagtg gtgttaattt
     3901 cgaccattgt cggcgaagcg aacttcctcg actggggctg gcgtattccg ttctttattg
     3961 ctctgccgtt agggattatc gggctttacc tgcgccatgc gctggaagaa actccggcgt
     4021 tccagcagca tgtcgataaa ctggaacagg gcgaccgcga aggtttgcag gatggcccga
     4081 aagtctcgtt taaagagatt gccaccaaac actggcgcag cctgttgaca tgtattggtc
     4141 tggtaattgc caccaacgtg acttactaca tgttgctgac ctatatgccg agttatttgt
     4201 cgcataacct gcattactcc gaagaccacg gggtgctgat tattatcgcc attatgatcg
     4261 gtatgctgtt tgtccagccg gtgatgggct tgctgagtga ccgttttggc cgtcgtccgt
     4321 ttgtgctact tggtagtgtt gccctgtttg tgttggcgat cccggcgttt attctgatta
     4381 acagtaacgt catcggcctg atttttgccg ggttactgat gctggcggtg atccttaact
     4441 gctttacggg cgttatggct tctaccttgc cagcgatgtt cccgacgcat atccgttaca
     4501 gcgcgctggc ggcggcattt aatatttcgg tgctggttgc cggtctgacg ccaacactgg
     4561 cggcctggct ggtcgaaagc tcgcagaatc tgatgatgcc tgcctattac ctgatggtag
     4621 tggcggtgat tggtttaatc accggcgtaa ccatgaaaga gacggcaaat cgtccgttga
     4681 aaggtgcgac accggcggcg tcagatatac aggaagcgaa ggaaattctc gtcgagcatt
     4741 acgataatat cgagcagaaa atcgataata ttgaccacga gattgccgat ttgcaggcga
     4801 aacgtacccg cctggtgcag caacatccgc gaattgatga ataagctgaa acggatggcc
     4861 tgatgtgacg ctgtcttatc aggccaattg aactcttaag gttcacttaa tctctgacgc
     4921 gcatactctc ctccaggtta acggaggaga gtgcaatgaa aaaccgtgtt tatgaaagtt
     4981 taactaccgt gttcagcgtg ctggtggtca gcagctttct ttatatctgg tttgccacgt
     5041 tctgatcttt cttcagccgt acccaggccc gcgtgccgga agtctcttgc cggttttgca
     5101 ggaaaaactg cccgtgatgc aactgtgtaa tgcggctgac aatacttaac cccagaccaa
     5161 tcccgccata acggctgtcc atacgtacaa acgctttact caactccccg catttactct
     5221 catcaatacc tggtccttca tcttcaactg ccatgaccgc tccgccatct tcttgcagct
     5281 taatcataat gttgctgcct tgcgggctgt aacgatgggc gttttctacc aggtttcgca
     5341 ataacatccg cagcagggtt gcatcaccct gaacggtgat gtcggcggcg ctctctggca
     5401 atagcagggt ttgctgtcgc tggtcgagca tggtactgag ttcgtcatac gaggggagaa
     5461 tgacatcttc cagcagtttt acatgttgat aattaccgga agaaaatgac tgtccggcac
     5521 gcgccagttg cagcagctgg gagacgctct ccatcatctg atcaagccgt gccactaacg
     5581 gtgctacatc aatgtgatgc gttttcgcca gcagttccag atgcaaacgc accccagcca
     5641 gtggcgttcg cagttcgtgc gcgacgtcag cggtaaacaa cctttcgtta tccagcgtgc
     5701 tggtcaggcg actgaccaga tcgtttaacg ccgaaaccac cgcttcgatt tcgagggtgg
     5761 cgctgtgaat ggcaatgggc gttaagttgt cggcagtgcg cgcttccagc tctttttgca
     5821 gctccgccag cgggcgggtg atgcggcgca ccgcctgata gcagataaat agcgtcaggc
     5881 tgaccataaa gacgccgggg acaatcaggc tggcgaccgc ctcgcggatc tcacgcatga
     5941 tgtggcgatc gttgttgcga ttgtcgcgta gcgcctgctc aaacagctga atctgctcgg
     6001 tactttcatg ccatagccag aagacgctga tcagctcaaa caccaacaaa atggccccga
     6061 tggtcaatat cagccgttgg cgcagcgata ttggtcggcg cagaaaatgc atcagattca
     6121 attagttttc ctcattcgcg accagcatat agccaaagcc gcgcacggtg cggatacggg
     6181 ctttgcccac tttgtcgcgc agattgtgga tatgcacttc cagggtgttg gtcgagggtt
     6241 cattgtccca gttatagatg tcgttgtaga gaatttcccg atgcaccgga ctgcctgctt
     6301 tgagcattaa ccgtgacagc agagcatatt ctttgggcgt cagaatcaac tcttcaccgc
     6361 ccatccatac ctgacggcga cccatattca gcgtcagatt gccaacaatc agctcacttt
     6421 cgccctgatt attatggcgt cgtagcaggg cgcggatacg ggcgtgtaac tcttccagcg
     6481 caaaaggctt caccagatag tcatcggcac cgacatccag cccagcgatt ttgtcggtca
     6541 gcgtatcgcg agcggtgagg atcagtactg gcagggtgta ttttttctgc cggatacggg
     6601 cgagaaaatg cagtccatct tcgtcgggta accctaaatc cagtaccacc aggctgtaat
     6661 gaccggcctc aaggctttgt tccgccatcc gcgcggttgt cacgccatcg cacgcgtagc
     6721 cttcagtttg cgccgccaga atcaatccct gcaataacag cgtatcgtct tcaacaatca
     6781 gaattttcat tcactcactc tcctgcaagt ttgcagaata tcatccgcag cctggtaata
     6841 cttcgtctca acgccagtta atcccaatag cgtggagaat aaattgtctt gtgaatagtg
     6901 ttgcgtttgc gcctgttttt gcaggcagtt ctggtcaacc tgataccgtt tttgataatc
     6961 ctccgacagc cacagcagca tcggcacctg tttttggcta tccggggcga tggcataagg
     7021 cagaccgtgc agatagatgc cattttcacc taacgattca ccgtggtcag aaagataaac
     7081 caggctggtg gtaaatttat cctgatgttc tttcagcaga ttaatcgctt tatcaacaat
     7141 atagtcgacg taaaccagcg tgttgtcgta agtgttcacc agttgctctt tgctacaggt
     7201 ctggatctca ttggtgtcgc aggttggggt aaatttcctg aactgcggcg gatagcggtt
     7261 gtaataggtc ggaccgtggc tgccgatggt gtgtaagaca atcacgccat caccctgcag
     7321 gttattgatg tactcttcca gcccgtggaa cagtacttcg tcatagcatt cgccgttgat
     7381 gcactgacca ggcagattca gcgcggtgac gttctggtga ggcacgcggt cgcaggcacc
     7441 tttacagccg ccatcgttgt cattccacag cacgttgatg cccgctcgct gaatgatatc
     7501 cagcacgcct tcctggtgct gtgccagctc ttctttgtaa tgctcacgcg gcatatccga
     7561 gaacatgcac ggtactgaaa ctgccgttgc cgtgccgcaa gatgcggtat tagggaaata
     7621 gaccacgtta tctttcgcca gccgcgggtt agtttcacgc gggtagccgt tgagggagaa
     7681 gttctccgcc cgcgaggttt cgccgacaat caggatggtc aaatttttac gtttttcgtt
     7741 ctgcattaac gggttgcggt gggcgtcttc accaattcgc accagcggta gatttgccag
     7801 tcgctgatgg gagtaccatg accagctggc aacaatgctg ttagaggggc ttaaggattt
     7861 caccagctct ttgttattgc ggaacaacga ggcgtagtct ttataaaaca gtgcggcgac
     7921 cagcaaaatc agtagtacag aaaccagaat attggctcca cggaaaagaa cactgcgcag
     7981 acgcgaggtg gcaggtttga tttttatcca gcaggcaatc agcgcagcaa gcacgccgct
     8041 gaatcccagc gttaataaca tttgcggtgt catcagcgca taactttctg ccggagtggt
     8101 atcaataata ttggcaatca tcgagcggtc gatgacgatg ccgtaagtca ttatgaaata
     8161 ttgtgcagcc gcgccaacca gaataaacag gcaggccagt ggtcgattaa gccataagaa
     8221 agagcttagt gtcaggacaa tattaatcac gctgaaagcg acgaccggca tcgacaagaa
     8281 aaccagtacg ttatgcagcg aatccagcgg cagcgcctgc aacacctgtt taaaaaaggc
     8341 aatattcagg cagatagaga tataaaaagc ggccaacaat agccaggcga gtaaattcaa
     8401 agagggtctt tttagtaggc gcttcaacat cacggtgttt ccatcgaaca aagtgcgcat
     8461 atgctcgcaa agcaaaatta agccaacctt aagttcttaa ggttgacttt tatgtttgct
     8521 ggattaaggg gaattaatct ttgcttattg gtgcatctaa gggatacggg tttttatgta
     8581 gccggttgta attcagcgca tacatggcgg tgatgaccat cagggtgaca aatgaccaca
     8641 taacctcttt cgctccggac cccaccacgg cccagatgca gtagaggaag gcaatggtag
     8701 taactgccag atatgccggg cgtgctttac caaagtgacc gtgtccgagc agcagtaacg
     8761 ccgcacaggt gtaaagatat ggcaccagtg taaagatgac cgagacggaa gaaaccagac
     8821 cgaactcttt ggtcgcgttt ggtgaaatgc tgctgagctg gaagatggtc atcaaaatac
     8881 cgacgataat cagccccgcc actggcgtac ccgctttatt tacacgggca aaaattggtg
     8941 ggaacagtcc gtcatcggca gcggctttcg ccgtttgacc cgccagcaac gtccagccgc
     9001 ccagtgaacc taagcaaccc gcagctgcgc agaaggaaac aatggccccg gcggtgtcac
     9061 ccaacgccat ccgtgcggca tcaccgaatg gcgaagcaga aacgcgcagt gcggcattag
     9121 ggatcatccc cataatcgcg gtggtagaaa gtacatagca aacggcggca atcaataccc
     9181 caccaatggt ggcgataggg acattgcgtt tcgggttttt caccacacct gcggcaacgg
     9241 aggcactttc cacaccgatg aacgaccaca gcgtaacgtt aagggtactt tgaattgcac
     9301 cgaaggtgcc caggccgctg acgttccatg ccgccatata ggtttcacca cggaaccaga
     9361 accagccaaa tacggcaatc ccgacgatgg ggatcagcgc cagcacggtg gcaactgcct
     9421 gcacacgggt gatcattttc ggaccgacaa tgttcagcag gacgaagatc cacagcacca
     9481 cgacgcaggt gatggttaat accaatggat ctttcagaat cgggaagaag taacttaaat
     9541 atcctacgcc aatgaccacc atggcgatat tgccgatcca gcaggccagc cagtagagga
     9601 cgttggtttg ataaccgaga aacgggccaa agcagcggcg ggcgtaagcg taagaaccac
     9661 caggacttgg gtcgaggaac gacattttgg cgtataccat cgagagcccc agtgcaccga
     9721 taatcgtcac caaccatcca taaatggcaa tcccgccagt agaggccagg tttgcaggta
     9781 acagaaaaac acctgacccc ataatattcc ccgacaccat cagggtgacg gggattaagc
     9841 ccactttgtg agcatcagca tccgaagaca taattaaact cctgcgaagg cgagcttcgt
     9901 gacaataaat tacgtcatat catacgcctg cattcgatag ttgaattatt atcgaccggg
     9961 ttatcgctga tgccgttatt aatatagctc ttcgttaagg ttaaaaatca ggcgacagtg
    10021 cgttctctgt gctgactaac ataatgcagc ggcgtcatac cgtagaagtc tttaaagaca
    10081 gaaataaagt acgacgtact gttgtagccg caggactgtg atacctgaga gatattttta
    10141 ccgtccatca ttaattcatt tacggcataa cgcatgcggc aggtggtgat tatttggcta
    10201 taactggtgt tttcgctttt caactttttc tttaacagac ttgggctaag acataaacaa
    10261 ctggctacca tactaagatt ccagtctttg tgaatatcgc tttcaataat ttgataaacg
    10321 ctgtcactta cacaattacg taacatatac atcattaatg aaaggaattt tttactgtcg
    10381 agaaaacggg atagcaccgt gaataataat gcgcgtgttc gttccttttc tgactcagtt
    10441 tctgcgggca tcatgctgtg ttgcgcggca acgcgaaaga cgtctggcgt caggcatggc
    10501 agggtgagta tgggcgtagc gctacgttgc catactggta tttgcgagag atctttattc
    10561 agaaaccgga ggtaatcttt gatgatgtcg tgactaatat gagcaaccaa agtgttgtta
    10621 agagaggaga cgtcgataat attattttca caatttaata acgccatatg gttagcttta
    10681 aggctaatag gttctttccc attcaccctt atccagacat ctttttcagt caataaaaca
    10741 atacaaggtt ggtcgctgca aatcctcata catgtactcc tgagtgcaaa taaaagacca
    10801 gttctcgcta aagcaaagcg ataccgggaa atggttatat aattgtatga tgaatataaa
    10861 catgcataaa aataacgagt tttactaata ttacaaattt ttatagtaat ttctcatgta
    10921 catatttttt tacaggataa ataacaggca aaaaaaagcg cggggtgggg ccgcgcaaaa
    10981 agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc aaaaggccta
    11041 aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag tggttacgct
    11101 ttcacgcaca taacgtggta aataccgtca ataatttcag tcccttcagt ttcgtgttca
    11161 aatccaggga aatggtggtc ccaggattgc agcgagcgta aataacttac ttgcggactg
    11221 tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg atacgggata
    11281 actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc gacattgttg
    11341 tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccatcggcag gccggaatag
    11401 gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat ggtgtcaccc
    11461 agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag ttccggcatc
    11521 acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag ggtgttaacc
    11581 agagtccccc atttcccacg ggttacgccc atagagaaca ggaacataat ttggaagtca
    11641 gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag cgccgccgga
    11701 acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat gctgacttta
    11761 attgggtcga gcatactcca gttatccgga atatctttga agccgtgcca gctttcgccc
    11821 ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc gtcagcaaag
    11881 tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg tttgaagaac
    11941 cagctaccgt cagcggtgaa ctctttatac agccgcgcca tcgcctgacg aaaatcaacc
    12001 gcttcgtcaa tcacttcttg tgtcagtgac aggccgctgt tgccgtccat catcgacacc
    12061 gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg catcatgtag
    12121 gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg aatataagaa
    12181 gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac ggtaggaccg
    12241 ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg gttgaaacgt
    12301 gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc cagcagatcc
    12361 tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac cacgcagtaa
    12421 gacggttttt gcccggcttt gtctttggtc agcgggcttt cactgatttt cttctgcaag
    12481 gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg gttgcggctt
    12541 ggcaccatat agaccggttt cgcgcctgtc agcatcaaac cttgttcgat ggatttatgg
    12601 cagttacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat gatggtgcgg
    12661 ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa tacgcgtgcg
    12721 gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc gagggaagtt
    12781 cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat agtagtcatg gtagaaacgt
    12841 ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc ccaggaatat
    12901 tcatggatgt cactatattt catcagcgcg ctaaacagtg gcggcaacag ctgctggcgg
    12961 tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc ttccagaatc
    13021 caggcgaatt catcgacaag ctccagcagg tcgcgatcca ttgcggcgag ggctttttcc
    13081 cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt accgatcaat
    13141 tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat caggcagtca
    13201 atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga tttaatcacg
    13261 gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc gacccaggtg
    13321 tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta cccggttatg
    13381 aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa gcgcgtgaaa
    13441 aacaaacatt tttgaaatat ttagttttat aaacaatacg ttatgtgatt attttaaatt
    13501 attttcactg cttattatcg acggctaaac tatttttttg gctgatactg atatcgtctt
    13561 tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg cggcgacatg
    13621 ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc tgccgtcagg
    13681 gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg gttgatgcgc
    13741 atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat ccccattgca
    13801 tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc ctgatcatag
    13861 ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg cgagacgctg
    13921 tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga gaggctaaat
    13981 cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc gttcggactg
    14041 tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt ggccagtgat
    14101 ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg ccaggagaga
    14161 aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt atctgttagt
    14221 tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt cactttttca
    14281 ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac ctgaccatgc
    14341 cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg ctggtattcc
    14401 gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat aaacgtatct
    14461 gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt gagggaggat
    14521 gactgcgagt gggagcacgg ttttcaccct cttcccagag aggcgagggg agcgtccgag
    14581 catcatgagg ccgaaaactc tgcttttcag gtaacttatt accataaact cagatttact
    14641 gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg gaggttttct
    14701 gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc atgatgactg
    14761 cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat attcttggtg
    14821 atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac attgatccta
    14881 cccgcttgga agagtcgcat attgtggtgc gtaagcttat ggattcagca ggggccagcg
    14941 gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat tttgtcgtgg
    15001 tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag gtctgtaagc
    15061 ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt atgcgcgcgc
    15121 tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc tgccccgatg
    15181 ccaccatgct caactatgtt aacccaatgg cgatgaatac ctgggcgatg tatgcccgct
    15241 atccgcatat caaacaggtc gggctgtgcc attcggtgca gggaacggcg gaagagctgg
    15301 cgcgtgacct caatatcgac ccagctacgc tgcgttaccg ttgcgcaggt atcaaccata
    15361 tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg cagttatgtg aatctctacc
    15421 cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccgaatatt catggcaata
    15481 ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac ttcgtcacgg
    15541 aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt cgtgaggatt
    15601 tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc gagcagctgg
    15661 cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat attaaaccgt
    15721 cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg agtgtgattt
    15781 acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt tgcgtggaag
    15841 tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg ctaccttcgc
    15901 atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc gaagctattc
    15961 ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat actgctgccg
    16021 tgctgggcat tgatgaaata tatgctcttg ttgacgacct gattgccgcc cacggcgact
    16081 ggctgccagg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc gggggattta
    16141 ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta acagcgaccc
    16201 gataccctat gagcatttca atgactacaa aactcagtta tggatttgga gcgttcggga
    16261 aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc gatgtcgtcg
    16321 ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg gatgctatta
    16381 acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt aagttcaaac
    16441 cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc tttagtgcgc
    16501 atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc ctctggggca
    16561 tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc acgctcgata
    16621 aacgtgagcg cgaacaactg gttccttatc cgcgtttttt tgccagtctg gcaggctttg
    16681 ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat cggggatttg
    16741 gctttcagat gttcactctg gtactgatcg ccttttttat tgtttcaacc atcatcactc
    16801 tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa ggaagccatc
    16861 tgacacttaa agccatcgtt gcgctaattt ataaaaacga tcagctttca tgcctcttgg
    16921 gtatggctct tgcttataat gtagccagca acattattac cggctttgct atctattatt
    16981 tctcatatgt tattggtgat gcggatttgt tcccctatta tctgtcgtat gcgggagctg
    17041 ctaacctggt gacgttagta ttcttcccac gcttagttaa atcattatcc cgacgcattt
    17101 tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg ttaatggcat
    17161 taatgagcta tcacaacgtc gtcctcattg tgattgcggg tattttgctg aatgtgggaa
    17221 cggcgctttt ctgggtatta caggtcatca tggtggcaga taccgttgat tacggtgaat
    17281 ataaactgca cgtacgctgt gaaagtatcg cttactccgt gcagactatg gtggtgaagg
    17341 gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt ggctatgtac
    17401 cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg attgctctac
    17461 caactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc ctcaatggcg
    17521 acacgctgcg caggatccag attcatctgc tggataaata tcgcaaagta ccgcccgagc
    17581 ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga cgtggacaat
    17641 agctaacgaa aatggcctga tgcgatatgt ttatcaggcc acgtcttaat gtaatgcttt
    17701 ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggca tcaacaaagc
    17761 gcacgttgtt atccggttat caagccaaag cgccgtagct ggcggcaatg aaagcgagcg
    17821 aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga acatatcgga ttgcgtgtcc
    17881 cactggtcgc cctgggtgcc gagaaaatca tccgctccct ggcccattgc cagcgccgcc
    17941 caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcagca gaccaggaac
    18001 gccaccattt tacgtccgcg cacgtacatc ccgcgcacga gaatttctcg tgccaccagt
    18061 gcaggcacca gcccctggaa aaaatgcccc agcttgtcat acggattacg gctcaacccc
    18121 agccattcct gcacctcaaa accaacgggg actttcgcgt aggtgtattg cccgccgacc
    18181 atcaggatga tggcgtgaaa gaaaatgagc gtatagagca gcggcgttaa cggataacgt
    18241 ttagcggtgg caagcagtag cggcacgaca ataatcaccg gtgtcacttc catcagccag
    18301 gtgagtttgt catgggccga aatgccggta taaatcagga ttaacgttag cgccagtgcg
    18361 ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca aatcagggaa
    18421 aatactgact attcaccagc aagcgtgaaa ttacaatcgc aaaagaggtg gcccaggggg
    18481 atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg cagttcgcgc
    18541 actgtttgtt gacgatttgc tggaagaagt cgttaccttt gtcatcgacc aggataaacg
    18601 ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc ggataagcga
    18661 cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg ccgatgctac
    18721 cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctgactg cggttacctt
    18781 tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacgtag gagtccatac
    18841 ggcctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt ttcgccggac
    18901 ccgcgtagta gatcgggtga tctttgatgt actgcggaag ttctttaccg gcgtcaatca
    18961 gctctttcag cttggcgtgt gcaatatctc ggcccacgat aatggtgccg gtgagcgaca
    19021 aacgagtgga taccgggtat tgcgaaagct gggcgaggat ctctttcatc gggcggttaa
    19081 ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag ttcttgtgga atgtactggc
    19141 ctgggttgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc gctttaatgt
    19201 tacggtcagc ggagcaggag acgcccatgc cgaccgggca ggatgcgccg tgacgtggca
    19261 gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc aaactgcgcg ccaagaccga
    19321 gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac gcctgaccat
    19381 gttcgttccc ttccgtcggc agttcatcgt aatagtgagc gcttgctaac ttgacggttt
    19441 tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac ggcgggcagg
    19501 ctgcagtacc gagggtacgc attttctcga cgaggaagtt tttcagtttg ccgggagtca
    19561 gcagggcttt ggtttcctgg tagagatacg ttttgttggc agagccgccg cctttcgcaa
    19621 cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc gcaggcaggt
    19681 tagtgccggt gttgacctct ttgtacatgt ccagcgccgc attctgtgaa tagcgcaggt
    19741 tatcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca ccgccgccgg
    19801 tccacacgcg ctggcctttt ttaccgacga tgatcgcggt gccggtatcc tggcaggtcg
    19861 gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagcgcc acgtacttgt
    19921 cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac ctgtttctgg tgtgccgggc
    19981 ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag cagggtcagg gcttctggtt
    20041 ccactttcag gatggtttcg ccgtcgaagt cggcaacgct aacgtaatcg gaagtgagta
    20101 gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata aagggtttgt
    20161 ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg tgccgcgtag
    20221 tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga agatccagcc
    20281 gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaagctgt ggttgatgac
    20341 gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca gatcgctcgg
    20401 ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga tgtatgccgg
    20461 atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag cctgagagtt
    20521 tacaaacttg gaaaccagca gcagaacaat ggcataggcc cacggatact ctttcaccat
    20581 ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt ctgccatcca
    20641 tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt cgttttttga
    20701 gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtca gcagcataaa
    20761 catctgaata accagtacca tcgacagcgg tttgccgccg aaggatggac gcaggtccga
    20821 atcagcacca agaagggcga ctacagcgat tgcaccgagg aaaatccaca ttgccagcca
    20881 gttgcttttc ggcagttttt tatcgagcag cgtcgcggta tcaccgtaaa catactcacg
    20941 gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat ctttaccgcg
    21001 gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa tggtgattgc
    21061 cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg acattaccca gcattgcaac
    21121 cagagacacg accgcaaccg acaccggact ggcgataatc cccatctgtg caccgataga
    21181 acttgccgcc atcggacgtt ccggacggat gttgttctta atggcgacgt cgtagatgat
    21241 cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagtg tacaggtcac
    21301 aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcagcttct cggcaatttg
    21361 cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcca ccaccgcaat
    21421 gataaccagc atgacatcaa ccggtggttt acctggctga aggtggaaga cgaagaccag
    21481 aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc tggcaccata
    21541 aaacagacat atcagtatta tgataagttg gatagtaaat aacatgtgtg aaccctcgcg
    21601 ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt gtatgaactg
    21661 ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa tacctcacag
    21721 taaatattgg ctgattaaag cagcggcaaa tttatgcact gtgataagcg gctttttcag
    21781 agagaaaaac tctctggcga tgggtttata gtttgtactg cgcagatgtt aatgttttgt
    21841 ttgctgttat cttattgata tttatcggtc tattttttgt tctcagggca gatatcatta
    21901 ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa atacacataa tgcgggtgac
    21961 ataatagtta attaactttt gttatcgttt tgaaattaaa aacaccgttc acctgaagag
    22021 atattaattt ttagcgatga tggagggata attatatttg atctggcaca agttttactg
    22081 atgaaggatg taacttgtgc caggggtatt ttgcattacg gtaataatta ttacttacag
    22141 ataaccagcg atccgttatt ggcaatattg tttcagtagt gagtagtgtt ctgcctgaat
    22201 acggtaacgg taaaccggac gccccgtgac gccataatgg atactggtga acaagatgtg
    22261 gcagttgacc agccagatga ggtatttacg gcaggaaaca cgcgagatgt taacctcgtt
    22321 agccagctcg tcggttgaaa actcatagtc ctgatgcgcg tcaatccact ggcacagcgt
    22381 gcgtaacgtc tgcggcgtta agccttttgg caagcgacga ggatcctgtt cgttggagct
    22441 gctgccgtgg attagctgat caagctcggc ctggtcataa tactgatgtt tttccagcgc
    22501 cattttcttt tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct ggaagggttt
    22561 gatcaggtaa tccacgacac cgtaatgcag cgaatcttta atggttgccg catcggctgc
    22621 ggaggagatg acaatcacat cacttttgca acgcgcgtta tgcaggacag gcagtaaatc
    22681 gagcccgttc tctttttgca tatagatatc gagcaatatc aggtcgatag gcgtatcgct
    22741 attgaagata atctctttgg ctttctccag cgtcgaggct gttccacagc attgaaagcc
    22801 tgggatttgt gctacgtatc ggcgattcag ctccgcgacc attgcgtcgt catcgataat
    22861 taatacattg atcatctgtt cgacctctcc ccgtcccagg gtatctggac aaaaaattgt
    22921 gtgaaaatcc cgggttccga ttccacggcg atgctgccgc cgagattttc tacctgttgt
    22981 ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg tcgagacacc tttgtcaaaa
    23041 atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat cgttaacttc gcagtgcagc
    23101 cagccgtgac ggtagtgcaa tgttacgcta atttcgccac cgggttccgg ccctaatgct
    23161 tccagcgcgt tttctatcag atttcccaat gtggtaatca gcgtcgcgac ctggtcctca
    23221 ctgccgctgt ctggcagctg gctttcactg tttaaaatca gcgtatggcc taaatcggtc
    23281 gcgcgattaa tcttgctgat taaaaaacca gcgataaccg gagatttgat cttacccagc
    23341 agagagccaa tctcttcctg atagttattg gctgttttga gaatgtaatc ttccaactgc
    23401 ttataactct tcagatgcaa taatccgaga atcacatgca atttattcat aaattcgtgg
    23461 gatcgttcac gaagtgcatc agcatagttg accagaccgt cgagtcgctg catcagttta
    23521 cgaacttcag ttttgtccct gaaggttgaa atggcaccga tgataacgcc attactgcgc
    23581 accggaacgg tgttgatcag taatagccga tctttaatcg taatctcttc gtcgcggcgc
    23641 ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct gtgaccatga gtggctgagc
    23701 gtcgacagtt tctcatcgtc ctgcgactta cggtaattca gcaattcttg tgcggcatcg
    23761 ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga cgccttcttt gatagactgc
    23821 aacatggcct ggcgctgctc aaacagcgtg gagatttcgt agggttccag gccgaaaagg
    23881 atttttttca gtaccttaac cagaatgcag gtgccaatca gtccgaccag catgccaaat
    23941 aataccgacc agataatgct ccagcgactg tcattgattt gttgggtcac gcggcttaac
    24001 tcaaggccga tcgccaccac gccaatttgt ttatgatttt catcgtagat gggggtaaat
    24061 acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga cattttcttt gccattcagc
    24121 gctttaagga tgtcatcacc tttaaatggc tgaccaatac gctgggcttc aggatgcgag
    24181 tagcgaagac tttgcatatc ggtaacgaca ataaacagca gatcgttgcg tttgcgcacg
    24241 gcttccgcga tggcctggat tccgctctcc tgcggttttt tctgcaagcc ctgacggatt
    24301 tccggcgagt cggcgagggt acgcgccact gccagtgcct tgttggctag cccgtctcgc
    24361 gtcatatcac tgatttgcga gaagtaaatc agatgcacca ccaatagcac cgagaacagt
    24421 accgcactga ccattaagat cactgtggta ctcaatttca tcggacgttt gcgtaacatg
    24481 cggtagggca atgaatgtct catcagcttc cttgtgtgac aaatttctta agcattatct
    24541 ctgatgaggc gggtaattca aagggagtaa gaatgattgg ctatataggg gaagagactc
    24601 tggcaacgga aactgccagt gctgtatgaa gattccaggg ctatgcttat agcgataatc
    24661 atactgatga gagagggaaa gtcatggatc aggcgctact ggacgagggt tatcgctgtt
    24721 ataccggcga aaagatcgat gtctatttca acactgcgat atgtcagcat tctggcaatt
    24781 gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa gccgtggatc atgccggatg
    24841 aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg cccgagcggc gcgctgaaat
    24901 accgtcataa ataagcgagg gtaaaatgga aatacgcgaa ggccacaata aattttacat
    24961 taatgacgaa cagggcaagc aaatcgctga aattgtcttt gtgccgaccg gagagaattt
    25021 agcgattatc gaacataccg atgtcgatga aagcctgaaa gggcagggga ttggtaaaca
    25081 gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa cgaaaaatta tcccactatg
    25141 cccattttcg aaacacgaat ttgataaaac gcgggagtat gatgatattc gcagttgatg
    25201 ggagagtaca gagtcacgat atttttcatg ctctccgcgg agtgatgcag gagagcatct
    25261 gaagggtagg gggatgcaca aagaatgggc agagagagcg tttttttgtc ccaagtcatc
    25321 ccctttactg agcaaaaaaa agaatatctc ctatatgaga atcatcaatc ggggttaata
    25381 agttttgcgt ccccagagcg tttaatattg ataggagtca tattatggaa ggtaaaaaca
    25441 agttcaatac ttatgttgtt tcttttgatt atccatcatc ttattcctca gtgttcttaa
    25501 ggttaagatc attgatgtat gatatgaatt tctcctctat cgtggctgat gaatatggga
    25561 taccacgaca attgaatgaa aactccttcg caataacgac atcgttagcc gcaagtgaaa
    25621 tcgaagattt aatcaggctt aaatgcttag acttaccgga tattgatttt gacctcaaca
    25681 ttatgacagt tgatgactat ttccgtcagt tttacaagta gccaggaagg ataagaagaa
    25741 cataaaaaat ggcactattc tctaaa
//
LOCUS       DAMZII010000069        25496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZII010000069 DAMZII010000000
VERSION     DAMZII010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.069"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            162..1748
                     /gene="prpR"
                     /locus_tag="POA77_002707"
     CDS             162..1748
                     /gene="prpR"
                     /locus_tag="POA77_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDI6366108.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(1849..2124)
                     /locus_tag="POA77_002708"
     CDS             complement(1849..2124)
                     /locus_tag="POA77_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6366109.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            2271..2942
                     /locus_tag="POA77_002709"
     CDS             2271..2942
                     /locus_tag="POA77_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDI6366110.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(2959..3204)
                     /locus_tag="POA77_002710"
     CDS             complement(2959..3204)
                     /locus_tag="POA77_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366111.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(3616..4431)
                     /locus_tag="POA77_002711"
     CDS             complement(3616..4431)
                     /locus_tag="POA77_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366112.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(4674..5723)
                     /gene="yahK"
                     /locus_tag="POA77_002712"
     CDS             complement(4674..5723)
                     /gene="yahK"
                     /locus_tag="POA77_002712"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDI6366113.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(5810..6766)
                     /locus_tag="POA77_002713"
     CDS             complement(5810..6766)
                     /locus_tag="POA77_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366114.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(6763..7734)
                     /locus_tag="POA77_002714"
     CDS             complement(6763..7734)
                     /locus_tag="POA77_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366115.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(7727..9211)
                     /locus_tag="POA77_002715"
     CDS             complement(7727..9211)
                     /locus_tag="POA77_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI6366116.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(9260..10243)
                     /locus_tag="POA77_002716"
     CDS             complement(9260..10243)
                     /locus_tag="POA77_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6366117.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(10497..10907)
                     /locus_tag="POA77_002717"
     CDS             complement(10497..10907)
                     /locus_tag="POA77_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDI6366118.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRDEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(11206..12588)
                     /locus_tag="POA77_002718"
     CDS             complement(11206..12588)
                     /locus_tag="POA77_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDI6366119.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(12598..13548)
                     /locus_tag="POA77_002719"
     CDS             complement(12598..13548)
                     /locus_tag="POA77_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDI6366120.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(13691..15109)
                     /locus_tag="POA77_002720"
     CDS             complement(13691..15109)
                     /locus_tag="POA77_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6366121.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(15109..16656)
                     /gene="fdrA"
                     /locus_tag="POA77_002721"
     CDS             complement(15109..16656)
                     /gene="fdrA"
                     /locus_tag="POA77_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6366122.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(16646..17509)
                     /locus_tag="POA77_002722"
     CDS             complement(16646..17509)
                     /locus_tag="POA77_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6366123.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(17549..18154)
                     /locus_tag="POA77_002723"
     CDS             complement(17549..18154)
                     /locus_tag="POA77_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDI6366124.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            18412..18909
                     /locus_tag="POA77_002724"
     CDS             18412..18909
                     /locus_tag="POA77_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI6366125.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            19001..19933
                     /locus_tag="POA77_002725"
     CDS             19001..19933
                     /locus_tag="POA77_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6366126.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(19975..21063)
                     /locus_tag="POA77_002726"
     CDS             complement(19975..21063)
                     /locus_tag="POA77_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6366127.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFINIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(21205..25188)
                     /gene="ehaA"
                     /locus_tag="POA77_002727"
     CDS             complement(21205..25188)
                     /gene="ehaA"
                     /locus_tag="POA77_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HDI6366128.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         6247 a         6784 c         5905 g         6560 t
ORIGIN      
        1 aattaaatgt ttaatttcaa tttgttagag ataaagtcat gaactaatcc gcaaatatgc
       61 gtttcagtta acgtttcagg caatgtttca ccgcgtttca ttgcaacaat tatgaaacaa
      121 gactaaaccc aatattcggt ttcttaactt tgcggtgcgc tatggcacat ccaccacggc
      181 ttaatgacga caaaccggtt atctggacgg tatctgtaac gcgcctgttc gagctgtttc
      241 gcgatatcag cctcgagttt gatcacctgg cgaacattac ccctattcag cttggctttg
      301 aaaaagcagt gacctacatc cgcaagaaac tggcaaacga acgctgtgac gccatcatcg
      361 cggctggctc taacggcgcg tacctgaaaa gccgcctgtc agtgccagtt attttgatta
      421 aaccgagcgg ctacgatgtg ttacaggcac tggcaaaagc cggaaaactc acctcttcta
      481 tcggcgttgt cacctatcag gaaaccattc cagcgctggt ggcgtttcaa aaaaccttta
      541 atctgcgcct cgatcaacgt agctacatta ccgaagaaga cgcccgcggg cagattaacg
      601 agctaaaagc taacggcact gaagcggtgg tcggcgcggg gctgattacc gatctggcag
      661 aagaagccgg aatgaccgga atttttatct attccgccgc caccgtgcgc caggcgttca
      721 gcgatgcgct ggatatgacg cgcatgtcgt tacgccataa cactcacgat gccacccgca
      781 acgccctgag aactcgttac gtgctgggcg atatgctcgg tcaatcacca cagatggaac
      841 aagtacggca gactattttg ctgtatgccc gctccagtgc agcggtgttg attgaggggg
      901 aaacggggac gggcaaagag ctggcggccc aggcgattca tcgggaatat tttgcccgcc
      961 acgatgcgcg acagggcaaa aagtcgcatc cgtttgttgc cgtcaactgc ggggcgatag
     1021 ccgaatcgct gctggaagcg gagctgtttg gctatgagga aggggcgttt accggctcgc
     1081 gacgcggagg tcgcgccggg ctgttcgaaa ttgcccacgg cggtacgctg tttctggatg
     1141 agattggcga aatgccgcta cctttgcaga ctcgcctgtt acgggtgctg gaagaaaaag
     1201 aggttactcg cgtcggcggg catcagcctg ttccggtgga tgtgcgggtc attagcgcca
     1261 ctcactgcaa tctggaagaa gatatgcggc aagggcagtt tcgccgtgat ctgttttatc
     1321 ggctgagtat tttgcgtctg caattgccac cactgcgcga gcgggtggcg gatattcttc
     1381 cgctggcgga aagctttttg aaagtgtctc tggcggcgct ctccgccccg ttttctgccg
     1441 cattacgcca gggattacag gcaagcgaaa ccgtgctggt gcactacgac tggccaggca
     1501 atattcgtga actgcgcaat atgatggaac gactggcgct gtttttaagt gtggaaccga
     1561 cgccggattt aacgccgcag tttatgcaac tgctactgcc ggagctggcg cgcgagtcgg
     1621 cgaaaattcc cgctccacgc ttactgacac cacaacaggc actggagaaa tttaaaggcg
     1681 ataaaacagc agcggcgaat tatttaggca taagccggac gacgttctgg cggcggctga
     1741 aaaactgaac tgccgaatgc agcgcgaacg ctttatccgg cataaatttc aatagatgat
     1801 gatcaccgat aaatttacgc atcggggatg tttacatagg tttcagaatt acttcttctt
     1861 ataaatattt gccgtgccgt ggatcttatt gtcagtttga ccagaggtca gtaccaacac
     1921 atcagcccct ttttcatccg cttttttgat caaatcctct ttcgcatctg cggtcgacat
     1981 ttcattactg gttgaaatat cacctatttt ttcatactgc gattcaactt tttcaaattc
     2041 cgctttggtc atcaattcag cggcatagac attggtaacg gcaaacgcta acgcaccgac
     2101 taacatttta gagattattt tcatattacg gctccagagt tgttatcaca ctatcaccca
     2161 cccacattat tgggttgatt aataattagt tcagtcaggg cataaacgcc agtaaccgga
     2221 aataagacac tttgtgatct ctctcgcacc cgattatttt tggtagaaat atgatgaagt
     2281 tacttcactt atttatggat gaaatcacta tggatccttt gcacgccgtt tacctgaccg
     2341 taggactgtt cgtgattact ttttttaatc cgggagccaa tctctttgtg gtagtacaaa
     2401 ccagcctggc ttccggtcga cgcgcagggg tgctgaccgg gctgggcgtg gcgctgggcg
     2461 atgcatttta ttccgggttg ggtttgtttg gtcttgcaac gctaattacg cagtgtgagg
     2521 agattttttc gcttatcaga atcgtcggcg gcgcttatct cttatggttt gcgtggtgca
     2581 gcatgcgccg ccagtcaaca ccgcaaatga gcacactaca acaaccgatt agcgccccct
     2641 ggtatgtctt ttttcgccgc ggattaatta ccgatctctc taacccgcaa accgttttat
     2701 tttttattag tattttctca gtaacattaa atgccgaaac accaacatgg gcacgtttaa
     2761 tggcctgggc ggggattgtg ctcgcatcag ttatctggcg agtctttctt agtcaggcgt
     2821 tttctttgcc cgctgtgcgt cgtgcttatg ggcgtatgca acgcgttgcc agtcgggtta
     2881 ttggtgcaat tattggtgta ttcgcgctac gcctgattta cgaaggggtg acgcagcggt
     2941 gagcagataa tttactctct acgtaatcaa cctgatttgc cgaatcccgg ccatcggcga
     3001 tgatagcccc cgtaatgcgt ggtccttatt tccgataagt ttacttgagt ctttagcttc
     3061 ccatttcagc ccactctccc ctgtgtgggc ttttttcatt ggtctgaccc gtcctgaata
     3121 gccaaacaat ggcaatgttc cactttgcag ggatgataat aaggggatct gattcagaag
     3181 agttttgaaa agttggaccg ccatcgaggc ctcgaacccc gtaccataca acaatcctgg
     3241 ttgtcgtctg ctcttcctga tgagctaatg gcggtttttg atatgagcct tacagcatat
     3301 tttctgtcag gcttgagtgc gatgcgtaag gtacagaaaa gaccccatac aaacatgatg
     3361 gaattatgat ttttttcata tgaatgtcaa cccacacatt cacaaaacat ctgcatgaga
     3421 tcatttttta tacaccacat agctgcgaaa ccactgctgg tgctttttta agcgaagcgt
     3481 cgctgatcac aacaaaatat cggcgaaaag gcggtggaat gccatttagc atacggatat
     3541 acagatgaaa tgattacctg ctttgcgatt cactctctgg tggaataaca acttgcaggt
     3601 ggaacccttt ctgaactaaa gagtgtagcg gattttaacg tgcttattta caggataata
     3661 tctgtcacct aattcctgca ctctattcga tatccattca tttaatcgtg cgtcatctct
     3721 gtatgttttt acgtccagaa cagagagcaa taacatcacc agtgattcag gaaaaacagg
     3781 gtggatattt tttaagtgta ataataatcc atgcgcaagc gcctgcttaa tgggcgcgtc
     3841 tgtctccgga atagacgaca tttcgcgaag ccatttttcc acacgcatac actctttccc
     3901 tttagggaaa attctcagca aaggcatatg gtgttcaaga agaatacatt tgcaatctgt
     3961 tcgcactgaa tcgataaact gcactaattt cttaagcccg gtaaagcgtc cagcctgcac
     4021 atttgcttta atatcaagaa acgcatcatc ctggtcatat acccaaactg tccctttttg
     4081 attttcaagt atgcgaaaaa caaacttaac cgggtgatca gggtaatcca tccagtcata
     4141 aaatccaggg aaatcattaa actgcacatt tatggtgagt gcgttatcgt ctaaccactc
     4201 acagtctcgt acggcagcca tgagatgaat gagtttgtcg tcatcgacta aatcatcacg
     4261 acaatcataa ggataatcat aatattgttt aatcaaaacc ttgaatatat cgacacagga
     4321 aaccagggag cttttgatat cctctggtaa ctgattgttt ttaaccgtat taagtatgct
     4381 ttgttgcgta tatggcatca aattattgtg cggagctttt aatgatatca tcgcgatctc
     4441 tcttaaataa aaataaacgt cgcagataat aaagttctca ttaaaccagg cactatcaga
     4501 aatcgctcat aactaacgtg agaagtattg tgtactggag ggagttaatt taacaatttg
     4561 aatttacgtt ataggtttta ctgctacttt gatattattt cgctctcctt aactaaaaag
     4621 ttaagtggag agcgaagtta atatgttaaa ccacagggta tttattaatt ttttcagtct
     4681 gttagtgtgc gattatcgat aacaaaacga tatttcacat caccacgcag cattcgctca
     4741 taggcttcat taatttgatc ggcccgaatc atctctatat cagccacgat gccatgttcg
     4801 gcgcaaaaat cgagcatctc ctgagtttct ggaatgccgc caatcataga accggctatc
     4861 gcacggcgtt tcatgatcag gttgaaaact tccggcgatt tatgcggtgt cgcaggcgca
     4921 ccaaccagcg tcatggtgcc atcacgcttc agcaaggtgg taaaatcgtc gagattatgt
     4981 ggcgcagcta ctgtattcaa aatgaaatcg aaactcttca gatgagccgc catctcatcg
     5041 gcattgcgtg agttaacaac ttcatcggcc cccagggctt ttgccgcttc gcgttttgcc
     5101 tcagaagtgg taaatgccac cacatgcgcc cccatcgcgt gggccagctt gatccccata
     5161 tgtcccagac cgccgatgcc gaccacgccc acttttttac ccggcccggc ctgccagtga
     5221 cgtagcggcg aatacgtggt gatccctgca cacaacaaag gagccaccgc cgccagctgc
     5281 tcttgcgggt gacgaatacg cagaacatat cgctcatgaa cgacgatctg ttgtgagtag
     5341 ccgcccagag tatggcccgg ttcgtccggc gtcggcgagt tataggtgcc ggtcatgtga
     5401 tcacagtagt tttccagccc gtcttcacac tcttcgcaat gtttacaact gtcgacaatg
     5461 cagccgacac cgaccagatc gcccggcgca tgtttttcta cctgatcacc aacggctacc
     5521 acacgcccca caatttcatg acccggtacg caggggtaaa ccgtccccgc ccactcggaa
     5581 cggacctggt ggagatcgga atggcagacg ccacagtaag cgatttcgat tttgacatca
     5641 ttcggtcccg gttcacgccg tgtgatatcc atcggttcaa gtggttgttt agcggaatat
     5701 gcaccaacag ctttgatctt cattgtgttt actcctgatt agctatgtgt atttggcgac
     5761 gcctgatatg tcatcgcggc aaaacgcgtc cattgaatat agccaatatt taaaccaccc
     5821 gatccagccc caacatgccg cgaacctgaa tgaaacagag cagcaacgtt ccccataagg
     5881 ccatcgtcag gtaggggctt ataccgagaa tattgaagcc gctttcaata atttgcagaa
     5941 gaaacagcgc cagcaccata ccaattatcc gcccgctgcc gccgtccgga ttaaccccac
     6001 cgagtactgc cgcgaggatt gacaccagca agtacgattc gccataagag gccttagccg
     6061 agttgagttt cgacatcatc aaaaatgccg ctaccgcaca aagcaaggcg gaaatgacat
     6121 aaacccaaat cagcacccga cgagtgttaa tgccggagta aagtgttgct cgttcgttag
     6181 acccaatcag gtaaatggct ttaccgagcg gcgttttctc cagcaatatc catagcccca
     6241 gtgccactgc ggcaaacaac cacatcggta gcggaatacc gaaccattgc gcatgattca
     6301 accacagcac ccatgaggga taattggcaa tggcgctacc acctgtcacc agaatattta
     6361 tccctttgag caacgtcatc atcccaagcg ttgcaagaat tggcgacact cgaataccag
     6421 caatcaatac gccgttacat aaaccgatta tcaccgccgc gccagcgccc gccagtaacg
     6481 tagcgacaac cgtagcaata cccggtggat actgagtcgc cacccacgcc atcaccagtg
     6541 aacaggcgtt agcagtggct ataattgaca agttaatgcc accgcaaagc atggtcaccg
     6601 ccatagccaa cgccagaatg cccagcacgg gcatttgcga agcgacggac tggaaattac
     6661 ttatcgacca gaaaatctcc ggcatagtaa tgctgaacgc ggcaacagtt agcaccagca
     6721 ggccgatcaa gtaaaactca acgttattac gccacgattt tttcatagca gactccggtt
     6781 ctgatgctga ctccacgccg tggcactaat gctggcaacg atgatgatgc cggtgatcaa
     6841 tgtttgccag taagacgaga ctcccaataa atttagcccg ttttgcatca ctgccaacaa
     6901 caccacgccg agcaaagtac cggttaacgt gccgcgcccg ccgagcaaac tagtgccgcc
     6961 aagcaccacc gcagccagta ctgtcagctc ataacccaga agagaatcgg gggcgacagt
     7021 catcaccgtc cacgactgca ctacacccgc agcgccagac atcaatccca tatagccata
     7081 gacgaaaagt tgcagtttca gcacgctaaa gccgatgcga ctggctgatt cgcggttccc
     7141 gccaagggca taaattttgc gccctacact ggtgaaattc atgataaacg ctgttagcag
     7201 caccaccgta atcatggcga tcagcggcag accaaggcca tagtcatagc catcagcatc
     7261 ggtgtacttg aacaacataa ccccctgctc aaaccacggc ggaaagtcgt acagccacac
     7321 acctttactc aaccataaca gcaggccata gaaaatattc agcgtcgaga tagtgatgat
     7381 gatagaaggc acccgtaggc gattaaccag taaggcgtta attaagccaa gtaaaatgcc
     7441 gatgcagccc gccagtgcaa acgcgacagc gaagttgcca ccaatttttt gcagcaacag
     7501 caccatgccg tattgcgcaa tgatggtcat tgctggaaac gaaatatcaa ttccaccgga
     7561 aatcaacacc acaaacaaac cacaggcgag aatagtcaac atggcatagt tattggcgag
     7621 atcgtacaaa ttaccgaatg tcagaaactc gtcgctgcgc caggcaaggc caacaaacag
     7681 caccacaatc agtaatgcca gccagaattc atggcgtttt ttcagttcag ccatttacca
     7741 cctccgcaat cctggcctga gaactgctgt caggcaggaa gctatgggtg atctgacctt
     7801 tttgcattac caaaatccgg tggctttgat accacgcttc ttcaatttca tcgcagatca
     7861 tcaataccgc aatgccatga gccgccagat cgctgataat gccgtatatt cctgctttgt
     7921 tggcgatatc aacaccgact gtcgggctat cgagaatcaa cagtcgtggc ccaatcgcca
     7981 gccatttggc gatcgatact cgctgcgcgt taccaccaga aagcgtattc accggaagat
     8041 gaggatcaga gactttgatg gtcaatgcct tgattagcct gtccaccagg tcacgaactt
     8101 tagtttcact cagaaagccg aagcgatctt ttaccttgtg aaaaacagtg ctaatgatgt
     8161 tgtcttcaat agattgcgcc atcaccaaac cgcgcgacat gcgatcttcc gaaacataac
     8221 caataccgtg gcggatagcg tcctggttac tatgcagtgt aaccggctgg ccgttgatga
     8281 ggatctcgcc agcatccggt cgggtcatac caaacagact cagacataat tccgtgcgcc
     8341 ccgcaccaag caggccaaca atcgatacca cttcgcccgc ttccacccgc aagttaatat
     8401 ttagatattc cccatgacga cttaaattac gcacctccag caccggggtg ctggattttc
     8461 cctgccacaa ctctcgcacc tgatattcaa actcctggcc ggtcatcagg aaaccgagtt
     8521 gttttgtggt catctctgca gccggaaaag tcccgaccag ctcaccatct ttcagcactg
     8581 aaatacggtc ggaaacttcc atcacttctt ccagacgatg actgacgaag accacgcaga
     8641 tcccgcgttc acgcaattga tgcaccacct gtaataaccc ttgcacttcc tgatgggtca
     8701 gcgaagcggt tggttcatcc atcacgatca accgtgcgtc ctgcgccagt gcacggcaaa
     8761 tagccaccag ctgacagcgg gcaattgaaa gctcagaaac gagtgtatcc agtggcaatg
     8821 tgacgttaat actggtcatt gccgcctgcg ccacctcacg cagacgacgg cggttaacaa
     8881 acagaccgtg gtgatagtga ttcacggcga tattctccca gacactcagg ttaggaaata
     8941 aagagaggtc ctgataaatc acctgaatcc cctgcgccac cgaagcggcg ggtgtcagct
     9001 ttgaccagtt tttcccacca agcgtaattt ccgctccctc atccgggcga taaacaccgg
     9061 acataatttt aatcaacgtc gatttaccac agccgttttg cccggcaagg cagtgcactt
     9121 cacccgacat aaagtcgaga gaaacatcgc gcagcgctcg cgtggcatga aacgttttgt
     9181 tgatatgacg aagggaaagg aaagtttcca taacatcccc tggcaataag aatcggggct
     9241 ttcgccccga agaaatggat taataaagtg agtcgatatt gtctttgttg accagtaata
     9301 ccttatggaa acggataatt cgcttatcca tatctacgtc ggccttaccc agattctgca
     9361 tttctaatcc aggtttgatc tcttcaccct tcagcaacgt gctggcgacg gctgcgagtg
     9421 cgtaacctgc agaggccgga tcgtaagtaa tcccttcggt gatatcacca cttttaatca
     9481 gtgatgccgc ctgtgaaggg atcatcatgc catagactgc gactttattt ttcgcccgtt
     9541 tctctttcac cgcacgtccc gcgccaatcg gaccgtttga accaaaggag acaaccgctt
     9601 tcaagtcagg ataggttttc atcaggtcca gtgtggtacg acgtgagtca tccacactct
     9661 cggcaaccgg catgcggcgg gtaacttcat gcatatccgg gtaatgctct ttctggtatt
     9721 tcaccagcaa gtcagcccat aagttatgct gcggcacggt caaactaccc acgtaaatca
     9781 catagccgcc cttgcccccc atgcgtttcg ccatatgctc aacatattca gcggcaaatt
     9841 tttcgttatc aatgatttca atatcccagt tagcacttgg ctgaccgggg gattcgttgg
     9901 tcagaaccac aattccggca tctcgcgctt ttttgaatac cggttccagc acgttggcat
     9961 cgtttggcac gatagtaatt gcattaacct tacgggcgat taaatcctca ataattttaa
    10021 cttgttgcgg agcatcagta cttgaaggcc ccacctgtga ggcattaaca ccaaaggctt
    10081 tacccgcctc aaccacacct tcgcccatgc gattaaacca cggcatacca tcgactttag
    10141 aaatattcac cacgactttt tccgctgcct ggagcggcgc agaaattagc gcagcgccta
    10201 ataacagcga agacaccata ttgataacaa aacgtttatt catcatttca gcctcattgc
    10261 agggtagtca ggaagtgttc gcccaaatta gcgcgctgga gagaataaga attttcgaat
    10321 atttttatgc agaataaaca gccctatttc aggcagctat taaaaatatg gttataaata
    10381 tggacaaaac gggcagcaac aataagtgtg acattcatca cgtaaaaaat agcaaaataa
    10441 acaaaattag cgatatttta tgtgtgtgag tttatttcgc gtagataaat agttatctac
    10501 acaacgtcaa taagggcata ttcttcaaat ataacgtcca tttctggtga atagcttcct
    10561 tctgcctgga aaaaacgacg gtgttcatta cgccaatatt caagactgag gtcaccttca
    10621 ccttccttac gggcaagctc cacggtgaca tcagaaaagc gcagtaaatg caggccaatc
    10681 actctgataa cacatacagg ctcatcacgg ctattttcta caatcttata ctcaccaatc
    10741 atcggaaatg cgttgtcttc aatacatccc gccagcgaac cacaggaagc tgttttaaca
    10801 ccagtcgcaa tcaacgttgc gaggtgatcg gccaacgctg ctgaatcgcc aaaagtacaa
    10861 aaggaagcat ggggatattt ttgtgagaat ttttctttta tggtcatggc aatatttttg
    10921 cgattacgga aaaacaaatc acatcttaat tactgttggt aaccatcagc aagagtatca
    10981 ttggtttttc gatactgaat cttttttatc cgaagcaggt cacatttatg gcctgataaa
    11041 tacctttgtt aaaactcact tttgtgtatt tctctcaaat aatcccctgg aaattatgcc
    11101 gcgagattat aaagttcagg atcattcatt agcaaaccat tcccgcccca ctccctgggg
    11161 cttaaatctc ttacaattca ccgttgcagg cgtttcaaat gcgcatcaac ctgccacact
    11221 cccccacacc agttgccctt tatggaacgt tgcggttctc ggcgagatac gcgccaccgc
    11281 ctcggcggaa caggaggcgt ccaccagcac aaagctggca tcatcctgcg cttttggcca
    11341 tacacgctcg cctttttcat tcagcggcaa tatatcgcca gtggctagaa acagcgaacg
    11401 ggagaggttc tgttcgttag gacgaatata gagctgcgcg tagagattgg ctttttccag
    11461 catgtcgccc agaccataag gcgaccagtg gtcgataacg ctgtcagtgc cagtcatcac
    11521 ttttacgcct ttgtcgtgca actgtttgag cggcatatgc agcgtgccaa tcggcaccgt
    11581 cgaggcgata gaaatttgct gcgccgccat ccgattcgcc agcgcatcga cctgttgttc
    11641 gttgagcgta gccagcgcaa aggcgtggct gatggtcagc ttacccttca gctgtggcgt
    11701 tttctctacc gtttcaacca tataattgat ggctgccacg cccgacggag tggtttcgtg
    11761 caggtgaata tcgacgcctt tgtcgtagtc cagcgcaatc tggaacatgg tgtcgaggga
    11821 tttttccatc gcgccatcaa cactggtcgg gtccaggccg ccgacgtaat gcgcccccgc
    11881 ctgcatcgct tcacgcatca aaggttcaga tttcgacagc agcaaaccgt gctgcgggaa
    11941 ggcgacgatt tcacactcaa agcccgcctg acgtcgcgcc agcaccgcct gcaaattttg
    12001 cagatttttc aggccggaaa ctggttcgat attgcagtga ctgcgggcaa tggtggtgcc
    12061 tttcgactgc aatagatcga tcagtttttc cgcccgctcc tgcgtatacg gttgtaactc
    12121 cggcagcatt ttctgctcga gtttgatcat gtcctggata gtggtgcctg ccggacgatt
    12181 gagcgagcgc cacggcccgc cgtagaaggt tttgtcgaga tgaatatgca tgtcgcgagt
    12241 ggtgggtagc atcaacttac cgccagcgtc atagtgcggc agcgtggcgt ccggatgctg
    12301 cttgttctcg cgcagggcga caattttgcc gtcctgaatc tccacggtct ggcgcgccgt
    12361 gcgggtctgc accgccacac cgttttcgta gtcaaaaccg gtttccagca gcacgttatc
    12421 gagatagtaa tgcgggtcag tgattttcat ggtgttgttc gcttcgcagt ttagggtgcc
    12481 agctaccgcc gcatgggcga gcggcacaga ggtaccaaac agcgcggcgg cggtgaccat
    12541 cttaccgctc tggctcagaa attcacggcg gctattgctt tctttcatcc tacgtcctta
    12601 cgttttaata atatgggtgc ccgtttcacc gcgcagcgcc gcaggcaggc attccggcgt
    12661 ggtgataatc acttctttgc cgccctgctc caggaacgtc aggctggcga tgatttttgg
    12721 caacatgctg ccgggcggaa aatgcccttc ctgcatatag cgagtcatgg tggcaatatc
    12781 cacccgatcc agcgcctgct gctgcggttt accaaagtgg atacagactt tttcgacgcc
    12841 ggtggtgatc accagaatgt cggcgtgaat ttcacgggcc agcagcgcgg tagagagatc
    12901 tttgtcaata accgcgtcca cgctttggta atcccccgct tcagtacgca ctaccggaat
    12961 tccaccgccg cccgcgccaa ttacgacaaa accttgttgg atcagcgcct taatggcggg
    13021 tgcttcgaca atacgtttcg gttccggcga ggcgaccacg cggcgatagc cccgtccggc
    13081 atcttcaaca aaacaccagt cagggtttgc cttttgtagt ttgtcacgct ggctttcact
    13141 gaagaatgca ccaatgggct tggtgggatg ggcaaaacct ggatcgtttt tatccacttc
    13201 cacctgagtt accacggtga cggctttttt ctcgccgtga cgcgccagcc ggttattcag
    13261 cgcctgttgg atcagatagc cgatgccgcc ctgcgtatct gccacacagt tcgccagcgg
    13321 cgttaagggc agcccttcgc gctcgtgggc aatctccgca cggcgtaaat ccagcccgac
    13381 ctgcggcccg ttaccgtggg tcagcacaat gtcgtaatcg gaagccagca tttccagcac
    13441 cgtatcggcc acggctttca ccgcctccgc ctgatgctca atcgactggc tggcgttatc
    13501 tttgataatg ctgttgccac caatggcaac gaccacaagc tctttcatgt tgttatgtcc
    13561 gctacagagg gtgattaggt tatgaccaac gccagactgc ctgatgcgct acgcttatca
    13621 ggcctacaat tctcgggcca tccgtaggcc ggataaggcg ttcacaccgc atccggcata
    13681 aacatgtaag tcagacgccg taacgttcgc accagccgag aatggctttc tcgaagcagg
    13741 cgaatggtgg atgcacaatg cccgcgccaa tcatgccgac gcccgcatct ttatgggcga
    13801 tggcggtgtt gatcaccggc agaatgccgc tgctacccac gcgggtgatg tcgattgccg
    13861 acggcacgcc cataaaaccg agcagcggaa tggtgacgtt ggggttttcg ccaagggtga
    13921 tttcgcgcat ctgacgggag aaatcgatag cctcttccac cgtgccgccc accagtgcga
    13981 cgattgcagg cgcggtcgcc atggcaaacc cgccaatacc gtaggtttct gtgatggcgc
    14041 tgtcgccgat atccagcccc gaatcttccg gcttataacc agcaaacatc gggccgatga
    14101 cctgctgcgc tgggccggta aaccactgcc ccggcaggcc gctgacccgc aggccgaact
    14161 cgacgccgtt acgcgccatg gtggtgacca cggtgctgta ttcgatgccg tgcgccgcat
    14221 ccatcgccgc tttacacatc gccatccacg tcgggccgga gaagtagtcg ctgctggcga
    14281 caaactcgaa cacttcgcgc tgttgctcga cggaatagcc tgcctgaatg atccccggcg
    14341 tcagtgcctg aatcagcagc gtcgtcccgg cgttattgcg gttatggcac tcatcgccca
    14401 tatgcagcgc ctgcgccagc attaagcgca gatcgatctc accgataatt ttcatcgcgt
    14461 cgcgcagcat tggcccctgc acatcgcgca tccagttgag gcggtcgatc acgctctgat
    14521 cgttggcccc catacgcaaa atcttcgcca tctgctcgct catattggta taagcgatgt
    14581 tgccgtaggt tttgtttttc acgatgtgca taaacatcga ggccgaggta acgcccgcca
    14641 tcgatcccac gcagtcgtgt tcgtggcacg gcgagaaggt gatctccccg gaggccgcca
    14701 gttcagtcgc ctcatcgaga tctttcgcca gcccttcgaa caccaacgcc ccggtgaccg
    14761 cgcctttcat cgcgccgcac attttttccc aggtgatcgg cggcccggcg tgaagaatgg
    14821 ttttcggcgt catgcccggc accacgttaa tcgcctgatc aaaaccaatc agcaccggat
    14881 gcgactggat aatgcgctcc agcgcctgct ggttagcggc ggcgattttg tctgccagcg
    14941 gcgaatcggc aatgttatcc agcgcctgca ccacctgcat attgccctgc cccggcggcg
    15001 tccagtcgag ttgagtcact tctacatgct gctttttcag gtcatcgcta aacatggcga
    15061 taccgacgtt aataacgttc aacggttggc taaacagtga ctggctcatc aggcttcctc
    15121 ccctttgcag ataaattcac gcgccagcaa tccggtattg gtgctgctgc tcgccagaat
    15181 cactccggca tcaagcagca tctggctttg ttgttctaat gatggcgtat cgagatcggt
    15241 gcccagcaca taggcgagaa tgatcaactc gcgtccttcg gcggcggcga tcgctttcgc
    15301 ttctttgatc gcctcgatgg tggagccgac cggatcttca tgcgatccaa atccgagcac
    15361 aaaatccatc acgatcaccg ccacttctgg atcgcgcgct tcttcgatca agcgactgat
    15421 gcggttggtg gggtcaatca ttggatgcgg cttgccgttg gtgaagtcgt catcgccaaa
    15481 gtcgaggaag gtgtgtttga tgctgcggtt gatatcttgc aggcggaatt caggatcggg
    15541 ctgaatgttg ctgtagacat cgccatgttt ttccatcacc gcgaacatgg tttcgtcgca
    15601 cagcgtgccg ccgcagaaca ggccacgaat gtatttctgc tgcggttgca gacgcgcacg
    15661 cacatcagca atcaacggct ggttaagcgt atgcaggtcg agattttcct gtttcacgcc
    15721 ggagagcatc accgctttta gcgccgcctc tttgctgccg cgggcaaact gtagcccctg
    15781 ctcatccacc ggcgtttcaa cgcgaccgag gaagcagacc accaccggct tgcggcaggc
    15841 acgtgcgcgt tccagcactt tgcgggccac cgcaggcgcg ggaggttttg aaatcagtgc
    15901 gataatttca gtttgcggat cgttttccag catcccgatg gcgtcgagca tcatcaggcc
    15961 gccgattttc tcgctcaggt cgcgcccgcc ggtgccaatc agttgtgaaa caccgccacc
    16021 aaattcatga atgcggacgc tcagctcctg gctgccggtg ccggatgcgc caacaatacc
    16081 gatattgccg cgacgcacgg cgttaccaaa acagagcgcc gcgccgttaa taatcgccgt
    16141 gccgcagtcc ggccccatca tcagcagccc tttttcgtgg gccaactgct tgagcgccag
    16201 ttcatcttca actgagacgt tatcggaaaa cagcatcacg ttaaggtcgt tttgcagcgc
    16261 ctgacgcgct tcgcgagcgg caaacagacc gttaaccgaa atcaccgcca ggttactttc
    16321 cgggatatgc tttttggcgc tggcaatagt ggcgtaacgc gcctcgtgcg agccgctttg
    16381 cgctttgctg ttgaacagtt cttcaatctc caccagcaac tgttcgttgt ccgcgcccgc
    16441 tttgccgttg atgacaatca tcaggtcgcc gtttttcgcc tgttccagct ccggcgtcag
    16501 cagtcctaaa tttttcagta cacctttgtt catttcggtc gccatcgcca caaatgcctg
    16561 ctcgacgccg tcgagtttat ttgcacgcgt ggagatagac atcagcgaga cagaatcaaa
    16621 ataggtattc ggtttaatga ctattttaac tgacataatt ccctccgtag gtttggctca
    16681 gcgcacaacc atcggccatg ccatacagca tgtcgcagcc ggaactggag ccaatatgtt
    16741 taattttttc tattgcctga gttgcgttcc cagggatgtc ataaataatg ttgtgaataa
    16801 aacgatgaat attttcccgg caacgttgtt gtaatgcggc ttccagcgtt atagcactta
    16861 ataatgtggt gttatttctg ccgcgctgca taccgagata aaattcctct ttgtattttt
    16921 ccgacggatg cccgggaata aataaaataa gcgccagacc tgttaaataa tcgtcggctg
    16981 atggcgtaag gccaatccct aacccaatca tactggcgac cgctgcggcg atattttgtt
    17041 tttcgtttac tgcctgaata actgcccgtc gtttaatatg tagttcttta tttaacgcct
    17101 gataaaacgg attatcgccg cgataattaa acagggtttc attttccccc aggtgctgat
    17161 gaataatgtc gtgccactgt agccaggcga taaggtgaaa attcacagcg gtcatttgca
    17221 aggttgggga cagccaacgc cgacaacgac tcatctctat atgaagatgt tgaccaaccg
    17281 taataccttg atcgtcaaac tgaaccttat caccatgccg gaacagatca tcaaagtgag
    17341 tgagtgccaa ccgacaactg tttggcgcat tgtcgtactc ttcacaaagc agcgtcagca
    17401 actgctggcg agccgggagt gcgatattta ccgctctggc aaagacctgt tcaacctgtc
    17461 cttgcccccg ctgcgccaga aaatccgcat ccgcagttag cgcccacaca caccgtcttt
    17521 ctggtgtgtt gatagcaagc ctcccggttt atgcacctgc ggcaatcagt aactgcgcaa
    17581 tctcttcaaa gccccgttcc cgcgccagtt ccagtggcgt tttgccatat ttatcggtca
    17641 gatgcgggct ggcaccgtgt tccagcaata actgcacaat cgcctgctgt ttaataccac
    17701 catcattaag cacaatcgct tccagtagcg gcgtccagcc tacatggttg gtctggttaa
    17761 cgttaatctc cgtatgcgcc aaaagctctt ttacaatact taaatggcct ttttcacagg
    17821 caggcgtcag gccgacaccg ccaaaacggg ttacgcaatt aagatcgggt ttagccggta
    17881 aaataattcg tagtagagtt aaatcatcgt tcagacagct aattaaaaaa ggatttaaac
    17941 aggtatgatc ttgcttatta atatccgctc cggcatcaat taatgcctga acgcaagcat
    18001 attgctgata taaacttgcc agcgtaattg ccgttttccc ctgacgatcg caggtattaa
    18061 tatcgacacc cagcgcaagg caggttttta ctttatcgat atcaccctgt tgtgcagcca
    18121 ataaatagtc ggcaggtaga ttttttatac tcattcacat tcccctggtt tttgtggctg
    18181 ttttcgatga attgagaata gcaacgataa tctgataaaa gaacccgctt aaatttaatt
    18241 cgctgtattt cgcctttttg ttgaatgatg ttcaacaatc ttttcgcagt gtgcggtagt
    18301 gcaatatttg tttctttgac ccgataacct ttcactcaat caggttttat ttctctatag
    18361 tctgcacatg taagagtagc tttcgtccat gtctaaccag aggggaacaa gatgaacgga
    18421 ctcacggcaa cgggtgtcac cgtcggtatt tgtgcagggc tttggcagtt agtgtcttca
    18481 catgtcgggc tctctcaggg atgggaatta ttaggaacca ttggcttcgt cgccttttgc
    18541 agtttttatg ccgcaggcgg cggtaaatcg gggtttatca gaagtctggc ggttaattat
    18601 tccgggatgg tgtgggcatt tttcgccgcg ctggctgccg gttggctggc ctctgtaagt
    18661 ggactgtccg cgttctgggc gagcgtaatt actaccgtac ccttctcggc ggtagtggtc
    18721 tggcaagggc gtttctggtt actgtccttt attcccggtg gctttctcgg catgacgctg
    18781 ttttttgcca gcgggatgaa ctggacggtg acgttactgg gttttctggc gggtaactgc
    18841 gtggggataa tttcagaata tggtggacaa aaattaagcg aagcgacgac taaacgcgac
    18901 gggtattaaa agttattcaa aaatgattat ctatctttaa ccgatacctc tacagtcata
    18961 gctggagaaa ttcatagtag ataatggcga gaatggttta atgaactcaa tttttaccga
    19021 agagaatctg ttggcattta ctaccgctgc gcgttttggc agcttcagta aagccgcaga
    19081 agagttgggt ttaaccactt ccgccattag ctacaccatc aagcgtatgg agacggggct
    19141 ggatgtggtg ctgttcactc gcagtacccg cagcattgag ttaacggagt ccgggcgtta
    19201 ttttttccgc aaagctaccg acctgctgaa tgattttcac gccatcaaac gcagtatcga
    19261 tactatttcg cagggcatag aagcacgtgt gcgcatttgt attaatcagc tgctttatac
    19321 gcccaaacac accgcacgct tgttacaggt gctgaaaaag cagtttccta cctgccagat
    19381 taccgtcacc accgaagtgt ataacggcgt ctgggatgcg attattaata atcaggccaa
    19441 tatcgccatc ggcgcacccg atacactact ggacggcggc ggtattgatt acactgaaat
    19501 tggcgcgatt cgctgggctt ttgctatcgc cccggatcac ccgttagcct ttgtgccgga
    19561 acccatcgcc gaaagccaac tgcgtctcta ccctaatatt atggtggagg acaccgcgca
    19621 tacgattaat aaaaaagtgg gctggttgct gcacgggcag gagtcaattc tggtgccaga
    19681 tttcaacacc aaatgtcagt gccagatttt aggtgaagga attggttttt tgccggatta
    19741 catggtccgt gaggcgatga cgcaatccct gctggttacc cgacaaatcc ataatccgcg
    19801 ccaggattcg cgcatgttac tggcgactca gcattctgct acgggtcagg tgacgcagtg
    19861 gattaaaaaa caatttgcgc caaatggcat attaacgggg atctatcagg atttactgca
    19921 tcgggagaat taatcgctgc caacctcccg atgcgctgtg aatagcggtt tacatcaacc
    19981 acctgctttc attacccatt cagagataaa tttattaccc ggtactggcg gagagtacaa
    20041 atagccctgc aaaaagtgaa cgcctttacc gatcattaaa tccgcctgct cctgggtttc
    20101 taccccttcc gccacgatac tcagaccagg cttacgcgct agttcgacaa tattgtccac
    20161 aatatgaccg gagatttcgt cgacactcgc catttgcaca aatgacttat cgatattaat
    20221 aaaatcgacc gggaacgcct gcaagtaacg ataggtcgca taacccgtac caaagtcatc
    20281 cagcgcaaag gtaatgttgt gctgatgaag gctgtcaaat atcgctctgg cttctggcgt
    20341 taccggaata ggattacgtt ccgttagctc gagaaccagc ttgattttat cgttacctaa
    20401 tttcttaacc aggttcagac actctttttc aaatcccgct gccaaaaaac aacccgccga
    20461 gacgttgatg ccaatatgga aattgtccgg cagcaaatgt tttaccggca tcagaatatc
    20521 cgcagtctgt ttcatcagtt ggcgggtcat tatgacaata agaccggatg actccgccag
    20581 aggaataaac tgatccggtg ggataattcc cgtttgtgga tgttcccagc ggacaagtac
    20641 ctcacagccc gtcagtaccc cagtctgcgc gcagaaaacc ggttggatcc acggtttgaa
    20701 ttcgtggttt tccaacgcca gactgatggc ttcaggcgtg acgatatggt gacaacggtt
    20761 atcagggata taaaaattat ttttatttcc cgtgccactg atgacttgcg gatgatattg
    20821 aaaaaagata tcccgccaga aggtaatatc actctggatg cctagttttt catacagctg
    20881 cttcttttga tgtgaaattg tctttgcact gcgatttctg tactgtgaga tttctgtaac
    20941 cgacattccc tccattagca gcttgagtaa aagccgttct ttttcggaca cgataccagg
    21001 caacgtcaaa ttcaccgttg ccccgaactc ttccaaaaaa acactcaaat catgcgaagc
    21061 cattgaacac accttgatta aatcttgtgc gccagctaaa tctgcgagtc ttcactctga
    21121 ctgataaagc tggctgcaag aaaatgacac agagagccga attatctccc gttcggctct
    21181 ctctcctttt actcattatt cagatcagaa ttgccactta atgccaacca ttgcagaggt
    21241 gtcattatat cccctatcgg caacctgaac gcccacattc ccccagacat tcaggttggc
    21301 atttagctgt ccttccaccc cggtttttat ctcagcaata tttcgggctc catcctgagt
    21361 gacggacacg ccatccatac tggtgctgaa atccttactg ttatgcagcc agttcacttc
    21421 tacaaacggc tggaactcgc gggatttacc gtcatccatt ttatggtggc tcttcagcca
    21481 ggtttttacg ccaagtcggg tttgaacatt gccatcaccg ttgctatgaa cgagggttcc
    21541 gttgctttcg cggtgcttat cggctttgac tcccatccag gtaacctgtg cttgcggctg
    21601 aacataccat tcattacgcg ttccctggct gccattaaat tcagctaatt tgtgtttgta
    21661 tccagcttcc agtgaagcgg taaatccttt tgatttatag gattcacttt gtaagtcatc
    21721 ccctttcact gtgttatcaa accagctgta ctgcgcccaa ctgtcgagat acgcgccatt
    21781 acgcgactcg tcatcggcat accaggtggc atagaggccg gtgctatatc cgttcacact
    21841 cgcttttgca cgataaccgg ttcgcgagga aatggtttta ctgtcgctgt tgccatatcc
    21901 cgccatgacc ccaacatgcc agctgtcgct gccgttttgg ctccactgcg cgacatcgcc
    21961 tcccagttgc agaacatagc gattgctttg ggttttcagc tggccgctgc catcacgcca
    22021 tttattatga ccaccttcat ggcgcatcca catagtggtt tgtttctgct cacccgtcac
    22081 catgtcggta tagtacgtat tacccagacg ctcatgcaga cgcgtggtga acattgtatt
    22141 cgctgctgca aggttcgcaa tgtagctacc cgcctccggt cgcaggtcat tatccacatt
    22201 cagatccgga cccggcgtag gtgtcgggtt agggttcggc tctggattcg ggtttggctc
    22261 cggattcggc atcgggtctg gctccggctg cagttcagga gaatcactgc cgctggtcag
    22321 ataccagtta ccactatttg ccccttgtcc acgcgcgaga gtgtagtcat acgcccccgc
    22381 aacgatacga ccagcctgaa caaattcgcc ctcagactta ccgtctacat ggataagttc
    22441 gataccgtta agggttttcg cacctgtacc gccagcgtta ttcaccgtaa cggcagtcgt
    22501 gccgctagta tcgccctcga caaccaattt atcggttact gagtcatcgc cattcagtac
    22561 cgtattcatt actagttgac cgttattacc gtggtagttc cccttcacca ctaacacatt
    22621 gccagcttgc tgtcctgaca ttccggtcat cagcgtaccg ttgttggtca gattgccgcc
    22681 cagggtgtaa ttcacagacg cagccgctct tttttcagcg ctattattca acgccagggt
    22741 accgttgttc tcaacgtttg cgctacccaa tccctgcgca tgacttacca ggacattagc
    22801 cgaattagca atgttcacat tcgccgtcag gttgctgtta tcgcccgcga tgtccagcgt
    22861 gccgccctga atattcagct ctccgctccc cgttaacgaa ccggttgaag tgcccccgtt
    22921 ggtaaccgtc agactgccgc catttaagct cagcagtgaa tcggcgctgc cattgagcgt
    22981 gcccacggtc tggctgtgac cattcatatc cagttcagtc tctgccgcca gacgcagttc
    23041 atgggtatta cccaacacgt tatcgttatt caacaaaagc gtcccactgc gcagatcggt
    23101 aacaccggta tagtcgttgt ctttgttaga aagcgatacg gtctgaccct tctggctgct
    23161 gaatgccagg tcaccactgc cggtaatctt tgcgctgaga tcggcggcat tctcgctttt
    23221 accgttagag ctcagcacca gagcatcgct gtcggtagcg tgtaaatcaa gctgggtcag
    23281 gccgtaaccg atatacagac cgtcattgtt taccccactc gtcagacgat agtcgtaagt
    23341 gccgacggcc gctttttgcc cattctgctg aatatcacgt tgggcactgc tggagaggat
    23401 ttgcccattt ttatcctgca attgcagatt gcccgcatcg cccagaactt ccgcaccttg
    23461 cgcatcaacc aacttaatgg tgctgttacc atcatcgact tcagtgagcg ataacgcaga
    23521 gtcaatatca cgagatactg agcttacaac gtcactgtca gaaacctgaa tcacaccctc
    23581 gccgcgcaaa tccagcgttt tactaacgtt gaccgtccct tctgtcatct gtgcacctgc
    23641 tgtcagggca ccaaaatcaa cggttccgcc ggagaatccc aaagtgctaa tggagtgagc
    23701 gcccttaccc actgttacct ggcttccctg acccgcagtg atcccgcctc tctgcagaaa
    23761 ctcagcattc ttttccaggt taaatgtcgt gttctggaac gccagtgaac cctggaacca
    23821 gtctgtataa gcaaactcat cgaaggagaa ttcgtgattc cccatgttta tttcaacaat
    23881 acctctacct tgaagatcgt tatcaaatac ccaatcttta ttggcattga tttgcagttt
    23941 gccatcaaca tccaggtccg cacggccgat gttcttgtct tcagaaataa ccgcgacagc
    24001 accagaagcg acatcaatat atccgctgaa tgcatagtta ttgccggtca gtgccactgt
    24061 cccactgtcg atacttattg taccattgcc ggaaatctta ttggcaaagg tcatgctatc
    24121 gtcagccttc aggtgtaatt tcccgcggtt gttgatctct gctgtaccta tattttgggt
    24181 gatccctaaa gcgccatttt gctcaatttt gactttacca ctaaaggcag agttatcacc
    24241 ggttaagtaa gtcgtgccat tgagcgcatt gatatagccc aggccctcgc tgccactgac
    24301 aatatccgcg gaaatagtcg cgtttttatc cgtgtggttt aaggtgattt tcttatcgga
    24361 tgtctttgcc cacacaaact caattttttc ggcatcaata atccctgcag ccttcgcagc
    24421 acttccttcc tgtgcaccta acgctaactc agagttggtg cttaaactaa ttttcggtgc
    24481 ggatatttta cctccatctg atacgactaa tgttgtatca gaacgttggt cgtaataacc
    24541 caggctaata tgttcacgag cattaacttc accaccgtta ttaaccgaca ggaactgatt
    24601 accgccatat ccgccgtaaa cgcccataaa gaaggtccct gcatttacct gactaccttc
    24661 gccgcttaca ttcccctgcg tataagtcag acgactggtg cccttaacat gaattttgcc
    24721 gccattggag acattaaaat agccttgatc atgtctcatg cctgagtttg actcatacaa
    24781 atcatcagca tataactcgc tgccagcatt ggagacattc aggaaccccg tacctttcag
    24841 caaaaagtta agcgggccat tgattgttac ctttcctccg gtaatatcca acccaccacc
    24901 agaagcgaga gtcagacgcg gacgggaagt atctgtcgta ttgattacca gattggcatt
    24961 gtcagtcacc gtaatacggt aatcgatgct ctggttctct ttgccaaaat caatagtttt
    25021 atcctggttt acgatgacat catcggcgaa taccagagat gaatataaac ttatcgcgct
    25081 aattaaaaca gcccgggact tttttccttt tactctgctc cccagctcag agcagacgct
    25141 ccaacagtta cgggacctgt tccactttag ccgatatatt ttattcatca caatctccag
    25201 caatacatcc aaataaaacc aagattgcat aaacagcaac gcattaaatg ccattctggt
    25261 tactgttcat cgcatataaa aattcaaaaa ttttgcatag cattggcaac cacatatctg
    25321 agtatgtagt tcagagggta ataaaatagc aagttactac tgaaaactta ttttaagtta
    25381 acactcttaa ctttatcatt acattcctcc cagtctctaa cacctgcgca ttatatgtat
    25441 ccaggaactc aaatcaactc aaaatataaa caaaagcagg aggaatacat ttaata
//
LOCUS       DAMZII010000070        25340 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZII010000070 DAMZII010000000
VERSION     DAMZII010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.070"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(8..301)
                     /gene="cas2"
                     /locus_tag="POA77_002728"
     CDS             complement(8..301)
                     /gene="cas2"
                     /locus_tag="POA77_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDI6366129.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(298..1221)
                     /gene="cas1e"
                     /locus_tag="POA77_002729"
     CDS             complement(298..1221)
                     /gene="cas1e"
                     /locus_tag="POA77_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDI6366130.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1218..1868)
                     /gene="cas6e"
                     /locus_tag="POA77_002730"
     CDS             complement(1218..1868)
                     /gene="cas6e"
                     /locus_tag="POA77_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDI6366131.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1850..2596)
                     /gene="cas5e"
                     /locus_tag="POA77_002731"
     CDS             complement(1850..2596)
                     /gene="cas5e"
                     /locus_tag="POA77_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDI6366132.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(2607..3662)
                     /gene="cas7e"
                     /locus_tag="POA77_002732"
     CDS             complement(2607..3662)
                     /gene="cas7e"
                     /locus_tag="POA77_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDI6366133.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWAMAEKGTEQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3674..4210)
                     /gene="casB"
                     /locus_tag="POA77_002733"
     CDS             complement(3674..4210)
                     /gene="casB"
                     /locus_tag="POA77_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDI6366134.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(4207..5769)
                     /gene="casA"
                     /locus_tag="POA77_002734"
     CDS             complement(4207..5769)
                     /gene="casA"
                     /locus_tag="POA77_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDI6366135.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEVEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRFVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVNLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5867..8566)
                     /locus_tag="POA77_002735"
     CDS             complement(5867..8566)
                     /locus_tag="POA77_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDI6366136.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCIEQRQDPEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            complement(8761..8913)
                     /locus_tag="POA77_002736"
     CDS             complement(8761..8913)
                     /locus_tag="POA77_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI6366137.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(9178..9912)
                     /gene="cysH"
                     /locus_tag="POA77_002737"
     CDS             complement(9178..9912)
                     /gene="cysH"
                     /locus_tag="POA77_002737"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI6366138.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(9986..11698)
                     /gene="cysI"
                     /locus_tag="POA77_002738"
     CDS             complement(9986..11698)
                     /gene="cysI"
                     /locus_tag="POA77_002738"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDI6366139.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(11698..13497)
                     /gene="cysJ"
                     /locus_tag="POA77_002739"
     CDS             complement(11698..13497)
                     /gene="cysJ"
                     /locus_tag="POA77_002739"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDI6366140.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            13816..14178
                     /gene="queD"
                     /locus_tag="POA77_002740"
     CDS             13816..14178
                     /gene="queD"
                     /locus_tag="POA77_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI6366141.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            14256..15527
                     /locus_tag="POA77_002741"
     CDS             14256..15527
                     /locus_tag="POA77_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6366142.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            15518..15778
                     /locus_tag="POA77_002742"
     CDS             15518..15778
                     /locus_tag="POA77_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI6366143.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            15795..16370
                     /locus_tag="POA77_002743"
     CDS             15795..16370
                     /locus_tag="POA77_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI6366144.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(16518..17378)
                     /locus_tag="POA77_002744"
     CDS             complement(16518..17378)
                     /locus_tag="POA77_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI6366145.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(17375..18154)
                     /locus_tag="POA77_002745"
     CDS             complement(17375..18154)
                     /locus_tag="POA77_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDI6366146.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(18132..19541)
                     /locus_tag="POA77_002746"
     CDS             complement(18132..19541)
                     /locus_tag="POA77_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6366147.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(19563..21017)
                     /locus_tag="POA77_002747"
     CDS             complement(19563..21017)
                     /locus_tag="POA77_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI6366148.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(21087..21872)
                     /locus_tag="POA77_002748"
     CDS             complement(21087..21872)
                     /locus_tag="POA77_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6366149.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            22191..23468
                     /locus_tag="POA77_002749"
     CDS             22191..23468
                     /locus_tag="POA77_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6366150.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQFSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            23495..24973
                     /locus_tag="POA77_002750"
     CDS             23495..24973
                     /locus_tag="POA77_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDI6366151.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTLAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         6092 a         6754 c         6216 g         6278 t
ORIGIN      
        1 gtctaactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca tccaaatcca
       61 ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg gtattggtcg
      121 cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc cagatcatct
      181 cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc agtagccaga
      241 ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg accatgctca
      301 tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga tggcaggcgg
      361 taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga cttcctcaat
      421 taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac aggctaatct
      481 gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct ttggcacaac
      541 cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt tgccactatg
      601 gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag atataccgta
      661 cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt cccagtcttt
      721 aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta atgcatacgt
      781 ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc gagctggcgg
      841 tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta gatcttccgt
      901 taaagccagc tttgcctggt agagtaattt atctgcccgc gcccctccgg gttgtccgga
      961 ggagtaaacg cgaacgcccg cttcaccgac ccagaccagt agcgtcccca ccgtggcggc
     1021 tagatgcacc gccgcgtggg aaactctcgt tcccggttcg agcataatgc aggcgaccga
     1081 tcccaccgga atgtgcgtgc ggatcccggt tttgtcgatc agcacgaaag cgccgtccag
     1141 tacgtcgatc tgaccgtact ggaggaagat catggaggtg cgatctttta acgggatcgg
     1201 actcagtggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag accacagccg
     1261 aacgcccggc ttttgccata tccctggcta agacgttgca gaaataaccc gggatcggtg
     1321 accgtaagca tccccgtata atccacacta ctgaactgga tcagttgccg ggagttttcc
     1381 cgccggagtt gctgttgtcg gtaggcatca accgaggtat ccagtagtgt aaaaccactt
     1441 ctttccccct gcgctgccag ccagtccagc gccgcctgtt gttgatgcaa ccagacatca
     1501 cttccttccg cctgtcccct cacctgccgt ttcgcctcca tcagcagatc gtggcgcttg
     1561 cccgatttac agattgttgg attcgcccgc aggttgaaac aaagttgttg tccggtacgc
     1621 agctcgggcg caaatgaccg gcattctatg gtgaacgtat cactttccgc cgggcgttcc
     1681 tgcgagagga caaaaaagcg aaacgcgccc tggagttctt cccgacgata aagaaattgc
     1741 ctttctttgc cgccagggaa aagatcccac agccactgat gcatcacata ttcaccgcga
     1801 tccaccagat gcagcaactg cgcaggcgaa agctggccgg tatgtaaggt tattcttgag
     1861 aggtacatgg ttcctccttg ctgagccacg gcccctgatt gatggtgcgt tccccaaaca
     1921 accactgctg acgatttaaa ggaacatccc gacggcgtaa tattttgctc gcgaccaggc
     1981 cgtcgtgttc cccttcccac cagcattcat cctgaagttt cgggagtgag actttcagtt
     2041 tatggaaatg atcctgatac tgttggtatg cattacataa ggcatcacac gcgttgcctt
     2101 cgagcagtaa cggcgcaaga ggtaatgcca gcggatgact ttttcgcccc agataaagcg
     2161 gaaaaactgg atggcgtaaa ccgtcctgca actgttcgag gctgtaaggc gcatcggctg
     2221 ttgttgccac cgccaccatc caccaggcat cggtgtagta gtcgcgccgg gagataatcg
     2281 cgctcagaag atcagggtcg ctcaactctt cgcgacggct gaaataacgc gctttacgca
     2341 cctcttttgg catctggata gtgtgataat cccgtgccca gcgcgggtta cggctggcgc
     2401 aaaccaccag tgaatagtgg cgattaaacg cgtttaagcg ttcggtatca tcacgccgaa
     2461 tccctacccc ggcagccagc agccccagca atgctgagcg cgaaggcagt tcatgggtat
     2521 gacgcacttc gccgggggca tcgacgcccc aggatgccat tggcccatga agctgaaaaa
     2581 tcagatattg gctcataagc cgccccttac gcgcagataa agtccagcac gtccttcatg
     2641 cttccctgtt tgttcatcac gtcaaaactt gcgcattcgg tcttctgttc gtagaccgta
     2701 ttcatgtttt cgcgaagcgt tgtaatacgc tgcaccgcca cctctaactg ccgggtgcca
     2761 ttaatgggtt catagaaagc cgccgccaga gaacgcggtt gttcggtacc tttttctgcc
     2821 atcgcccagg aggcgtaggc acggctggca aagctgttct gtttgccggt tggggagact
     2881 ttaagcgcgg cttccgtaaa ggcgcgtatg gtctgattag ctaacgcttc gtcaccgccg
     2941 aggttttcga ccagcagatc tttatcaatg cagatatagg tgtagaacag tgcagaaccg
     3001 aatccggttt caccaagatg cccggcaccg gcatcttcag aagcctggcg caaatcatca
     3061 acggcggtga aaaaatcatc ttcgacaatc gtttcactga caccaaatgc gtgcgctacc
     3121 tggcaggcgg cttcaacatt aaactcgggt ttattcgcca gcatacgacc aaacatggca
     3181 atatctaccg ccatacggtc tttgcgtaac aacgcgagat cttcctcttt tggcgcgcgc
     3241 ttttcttctg caagctgatg ggccagcgct tttacggcgt caaattctgc cgggctgata
     3301 tgaactaatt gttcagtttc ggcgttagtg agcggatctt ttggtttttt gtcgttttta
     3361 gctttcccaa gataatccgc aatttttgcc gcccattcga tggctttttt ctcttcgatt
     3421 cctttctcaa tcaggatagt tgccgcctca cgcgcaatac gcccactacg aataccaata
     3481 tggcccgcca gtgcctgttc aaaaagtgca gaagtgcgcc acgcacgttt cagactttgt
     3541 gaggaaacgc gcagacgcgt tgcgccaccc agtaccacag tttttggcgc accagtatca
     3601 tcacggttaa ggttggcagc agggtaagcg gttaacaaat gaagctgaat aaatgttgtc
     3661 atgagatagt cctttattga ttttgttcgt tatcggcgtc gccagcctgg taatattcca
     3721 gcgcccagcg gatacggata aattcggttg gccgctgctg gcggcggtga tgattgagca
     3781 ggtcgtcgct ctcctggcac cagcggaaga ccccctctgc cagagagtca agattgacgg
     3841 aaccgtttaa taatctgact gcgcgccgta actggcgcag taattcatcc ggtgttttta
     3901 ctgccgagag gcgagtaaaa cgcccctttg acattaccgc cgccagctgc gcggcaaaag
     3961 gctgccgttc atcaatggct ttaacattgg cgctaactgc ggctatcagc gctagtgcgg
     4021 tgatacgcca ctcaggttca tcctgccact ttatttgtct gttcttcaga aacaggcgaa
     4081 atccatccgt cagacaaaca tcattaaccg tcgtactacg ccgcagactg gcacgttcgc
     4141 cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt ggctttgtgc tcctctttta
     4201 caatactcat tcagcagcct cctgcttttt ctccctggcg gctttagcac tttgcttctc
     4261 cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg cgttccaaat taacgggctc
     4321 ataaggatta gtgaatacac gctcgtcaaa atcctgacgc gcgaataacc aaatttcctt
     4381 attccatttg ctgagtaatt catccgcatc ctgaccttct tcaatttggc gcacgaacct
     4441 cagaaagcga tgctgagttt tgttccagaa gtcgatatcc acaaaactaa aatcacccct
     4501 tgcacctttt ggatcagaga accatgcttc tttcaatgca ctccgtaaca gacttagaat
     4561 ccgtgaagcc gtttgcgcag ccagccgcag ctttggtatc tggccttctt ttttattgag
     4621 caatagaggg aaatggtgtt cgtaccaaca gcgcgctttc atgttgtcga aatcataacc
     4681 aaatccccac aggcccactt ttgcctgttt cagactgctg gcattaaaga gtttcaccac
     4741 cagcgcggga agttccgtat tgttttctga tttacccgtt tcgataaggc ctaaccagtc
     4801 acgccagatt aaaccgcccg gttgtggttt aacggagtaa aactcgccac cctctttaag
     4861 tggtacacgg taaggcgtta agggatgctg ccacatggca taattcgcac cgtaattttt
     4921 ggtcgtcatc aggctcagga gcgcgtcgtt ctgctcgccg caaatatcgc agttgccgac
     4981 tgtcgtggta ttaaaatcaa tacgaatacg ccgcggcatt ccccagtacg cctggagttt
     5041 attaacctga tcatcggtta ccaccgcacc ggccagttcg ctggtgcgcg tcgggccaag
     5101 ccaggggaaa accagatcgt caaatttttt gggtagcggt aaatcggctt catcctgcgg
     5161 catcacgttg agccacattt tgcgccacaa gggagtttgt tgattgccct gatattcctg
     5221 taattcaatc agcgtggtca tcggcccacc gccgcgtaag ccggtgcggt agcctttacc
     5281 acctgacggc gcatttaact gtagggagaa cagagctaac gcagaacaat gtgggcacag
     5341 gtattccgtt acaccacgct tgataaagtg gtctttatta aacttcgttg tttgagcgcc
     5401 gggaatctca ggcagtagtg aagcgacctg aactttatct cccgtgagcg cctcaaaatc
     5461 ctgcataaat gaaggggaat ctggcccaaa ctggaaagcg tgttctaagg acagcaatgc
     5521 ttcccgtagc ttttcaactt ccagtccgtc ttcccagata tcatcccaac gacgatgatc
     5581 ttttggcgca aagctggttt gtagtagccc cagcaaaaac tgccatgccg ctccctggag
     5641 atctgcccgt ggcgcagcaa tatcgacaac attttcatcc gccagatcga caggcgccag
     5701 tttgcctgtc gttccatctt tgaaacgaac aggcaaccac ggggttgtca gaagtgaaaa
     5761 cgagttcata cgcatcctct cgtaaaaaag accatcccag gtcggttgtc catcattcca
     5821 tccttttcgg tgtatcaact attcagagag ccctgtcgca ggcatctcaa ccaaccttgc
     5881 cagtcaatcc ttccatagcc gaatacccgc aactttcgtc atcggtgact aaaatcacgt
     5941 ttgccatctc cggatcttgc cgctgttcaa tgcaccactg cctgaacgca tccccttcca
     6001 gtaaagaaaa ctcatcccga tgttttttcc accagcttcg gcgcactctg acaacgctca
     6061 tttcccatgc gtgagcaccg gtggcataag gcttcaccac accgtcaata caggtagcca
     6121 gccacaggga aacagactcc tcagccagac gtgtcgacag tttttccggg aggtaatcgt
     6181 tgatattggc ggcatagccg ggcctgaagt tcagcacaaa ctttttagcc cttgcgcgat
     6241 cacagtaata tttgcccact tgctcctgct cgctgcgggc aaatccttcc ggcattacca
     6301 cgtcctcacc gtagacggat tcaataagaa gccgggctga gtgtggcatc tgaatagcac
     6361 cttgctcacg cagcacacgc tgcgtcagcc agattcgccc atgatcggga tagacaaatg
     6421 cactgttacg catggcactg ccaaaccatt cgtcgccagg agagtcgtcc cagacgggag
     6481 ccagaatcag caattcaggg ggggaacgct cgtcctttcc gtcacgcttt aactgaccat
     6541 taatatcgcg gatatgccgc tgtaatcgtc ctgctcgctg aatcagcaaa tcaacagggg
     6601 ccaggtcgga gatcatttcg tccaggtcaa tatccacgct ttgctcaatt acttgtgtac
     6661 aaacaatgac ttgcgacgct ctctgtaaac tacaatattt accaaagcgt gccagtgtct
     6721 ccgtttcaat tcgctggcga tcgctaaaag caaagcggct atgaaaaagt gaaaggctgg
     6781 aagcaggaat gacgccgcgg gcaagcagct gacgatgaac cttaatagcg tcatcgacag
     6841 aattccggat ccaggcgatg catttcccct gacttaccgc cgattcgata cgcgcaatac
     6901 actcttgttc actatgaagc caacccacgc tgacgctacg ctcaacgtct ttgcgcgtcg
     6961 ctacccggtg tgagttcaca tcggatttcg tgacatgcgt cagccagggg taatcatcct
     7021 tttccaggaa cggagcttct tgcaggccct ctgtaccccg cgcaaaggca gcgacgagtt
     7081 tgtcgcgctg ctgttgagat aacgtggcag aaagcaaaat aacgctgttt ccgccacgcg
     7141 cctgccgctc gatcaaccct tcaagaatgc acgacatgta agcgtcacaa gcgtggatct
     7201 catcagccag caggattttg ttactcagcc ccagaagccg cagattatta tgtttgaacg
     7261 gcatcaccgc catcatcgcc tgatccagcg tgccgacgcc aatttcagcc agcaacgcct
     7321 tcttgttact gtcggcaaac caggccgcac atccctgact gaacgtttgt tcatccggtt
     7381 cttctgaccc gactaaatca ccagaccaga gtgattcatt aaagcggtcc attaatgtgc
     7441 gggcactatg tgccagcacc aggctggggc gggattctgg cgaatagaaa gcaagccagg
     7501 ttttgaccag ccgatcgtac atggcattgg ccgttgccat tgttggcaga ccaaaaaaca
     7561 gcccctgagc tttccccgca gctatcaacc tgtgcgccag gataagcgcc gcttctgttt
     7621 tacctgcgcc agtcacatct tccagaataa ataactgcgg tccttgctgg ctgatatcca
     7681 gatccagcac cttttgctgt aacggtgtcg gattctcaat aaaaggaaac agcgtattaa
     7741 ttccgttgaa aggcgcgact tttgctttta gaggaaagac ggttaaggcg ttttgagcct
     7801 gaattaaagt tttctgccag taacctttaa tatccattgg gtgtgcgacg cgtggaaaaa
     7861 atcgcgttga cgaacccgtc cagtctgcga gcacgacggt tgcagagata taccaggaaa
     7921 ggtgttttat aagttcaatg ccctcatcat catcccagaa tgcggggatc tctatgagcg
     7981 gaaacagtgg tttgatttca aggagaaaat ctcgtgctgc agctttgtct tcaggcagaa
     8041 aattatccaa ttcatccata cgatcagggg gccgaccatg atgccctgtc gtcacgggca
     8101 tccacatttc tatcactcgc ctaagtttgc gcggagagag cgaagatgaa ggaagtaact
     8161 cctgacattc acttaaataa tgattccaca accagtagcc cagcgttgaa tgagagattt
     8221 tttcgtaatt ttttctggca ccttcctgaa tcttgagttc agggggcagg taaagttgtt
     8281 gaaaagagcg ggcaaatttt ccaatatcgt gccagcacag caaccaggcg aaaaattgag
     8341 ccgcctgttc cttgtcagaa atccctaatt gacgaaaata atcagccagc ccgaagcaat
     8401 ttcttttaac cattaaatag cccatcgcgg ctacatccag cgaatgccag caaagaaggt
     8461 gatagtcgtc gcccccgtct ttctcgccgc gtcgggtttt tccccagaaa tcaaaaaaag
     8521 tcacaatatt tttatccttc agtaaactta aaggatattt acgcatatat taaaaattat
     8581 ctgtgatata tatcattcaa taaaatattt acattatgaa taatcaaata attttcatcc
     8641 gtaatatata tgaataataa acataggcat aatattcaca taagcaatat aaattaaaaa
     8701 agggtgcagg agaccaaacc atcacaattc agtacgcggg gttttacccc cgcgtggtgg
     8761 ctacttcttc gattcgtaag cgagaactgc cttaaactcc ataccacgtt ctctgatact
     8821 caactcacac aacgagtcgc ctaccatcag cgtgaatccc agtactgtaa aacacagcac
     8881 tatgattgcc acaagggcat attttgtcag catgtcttgc ctccctgcaa agaagagact
     8941 aacattcgaa ttgtttgggt tcgaacgtaa gcctcaggtt gatagaaata tcgcctgggg
     9001 cttttgtcca tctggaacct cgcgaatgct taacgccaga cagcctcaag cacccgacgc
     9061 cattctatac ctgataattc ttccagcggc gttcacgttt aaaatcagga atactgctcg
     9121 caaaataaaa acgcccccat catttctgac agaggcgttt aatttatccg gcaatattta
     9181 cccttcgtgc aatccacatt ccctttttaa gccaaagaaa cgtgtttctt cttccgacat
     9241 gccaggttcc catttacggg ttgtatgggt atcaccgacc gataaatatc cttcatccca
     9301 taatgggtga tatttcaggc catgtttttg caggtactga taaatagttc ggttatccca
     9361 gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg gtaaattggc
     9421 gcggctgccg gattgttcgc ggcgcaggcc agcaaaccac gtttgcgcat tcagttcttt
     9481 cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa tgccttcaac
     9541 gccctgttcc cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt cggtagcgcg
     9601 gtacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc ggtaggtttc
     9661 cgggaacaga tagccggtgt cggtgaggat caccggaata tccgggtgaa tctgattcac
     9721 cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat attcaccggg
     9781 cagattatcc agcgcccagg caacgcggcc ttcagcgtcc agtttttcca gttcggcgtt
     9841 agtttctgcc agcgccagaa tgcgatctac cttcggcagt tcgttcaggg cgtttagatc
     9901 gagtttggac atagattcct cactgtttgc cttgcctgat gcgacgctca cgcgtcttat
     9961 caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga tcgagcaccg
    10021 ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt
    10081 tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg gtgatgtttt
    10141 ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc agattgtagc
    10201 gacccggtgc tttacccacc aggcccactt ccgccagcat cgcgcgacca caaccgttcg
    10261 ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc gccattaaat
    10321 tatcgatgtt gtcgataaaa gacggcagga aacgctccgc ttccgccatc gccagcgggc
    10381 aagtcgggaa tgacacgcag gccatcgagt tttcacgctg cggcgtgacg gcattcatta
    10441 aaccgctctc tttggcgatc ttctcgatct tcgctttctc gctttccggt acaccggcga
    10501 tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc gcgatctcca
    10561 gcaggccggt tttcagcgga cgccccggat aatcaaggat gcgaccattt tcgataaaca
    10621 gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatcg cctcgtccgg
    10681 tgaactcata tggacggatc ggttcaaatt tgatccccgc gcgacgctcc acttccgctt
    10741 taaacgtctc aacccccacg cgctccagcg tgtatttggt tttggcattt ttacgatcgg
    10801 ttcggttacc ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc agcgtatgct
    10861 ccagcggcag atagccaaac tcgctcgccg tgcgggcgta ggttttcttg ttgccgtgtt
    10921 cgatagaaag cccaccgccc accagcaggt tgaagcctac cagcttgccg ttttcggcga
    10981 ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc gttctgcggc gggatcacta
    11041 ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag gatcggttct tcatcagtag
    11101 tggcgacttt ttcctggtcg agccagatct ccgcatacgc gcgggtacga ggcaacagat
    11161 gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac tcgtaagggt
    11221 tcgaggtgca gagtacgtta cggttcatgt cgttagctgt cgccagcgca tcaagaccga
    11281 ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg tggaactgaa
    11341 acgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg gcaaatttgt
    11401 cgatcgcctg ccactgttta gtggtaatca ccccacccgg cagacgacag cgaagcagca
    11461 tcgcgtggcg cggctccagc ttctgttcag cacgttcggc gcggatgtcg cggtcatcct
    11521 gctgatacat gccgtggaag cggatcagca ggaagttgtc gcccttaaag ccgccggtca
    11581 gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca agcttcatgc
    11641 gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt tcgctcatta
    11701 gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt catccgccgc
    11761 ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct caacgtcttt
    11821 cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccatcattga tccagcgcca
    11881 cagctccgcg ccctgttcgc gcagtttgtc ttgtacgtaa actttttctt tttggtcgcg
    11941 cgaccaggca agatcgatac gtgtcagcac gccatctttg acgtagcgct gccactccac
    12001 ctgatacagg aagtcttccg taaagtgcgg attaccaaag aacagccagt ttttacctgg
    12061 cgcttcgtcg gcggcgcgtt gctgcataaa ggcgcggaac ggcgcaatac cggtgcctgg
    12121 gccaatcata atcaccgggg tttccgggtt agcgggcagg cgaaagttat cgttatgttc
    12181 gataaatacg cggacttcgc cctcttcttc cacgcgatcc gcgaggaagc tggaggcacc
    12241 accggcacgg gctcggcctt ccacgtcgta acgcaccaca ccaacggtga cgtgtacttc
    12301 gttctcgact tccgcctgcg aggaggcgat ggaatacagg cgcggggtca gcgggcgcag
    12361 cagattaatc agcgcttcgg catccagttg cgccggagag aaacgcacca tgtcgacaat
    12421 cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg gcagcagtgt
    12481 ttcgctgcgg gtaagcgtgg cgtaattctc aacaatgttg gcggtgttga cggtcagttc
    12541 gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg tgacaggttc
    12601 atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccggat cgttctgata
    12661 ccagacgccc agcgcgtcac ccggctggta acgcaggccc gagtcaccta agtcaatttc
    12721 gatatggcga acgtcttttt cagagttacg cccggtaatt ttctggttaa cagagaggct
    12781 agccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta ccgcgccagt
    12841 agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg
    12901 ggcgcgccac tcgctggcag cagcctggta ttcaacatcg gcatcgacac ggtcgagcag
    12961 gcgttcacca cccagttccg ccagcttgct gtcgaaatct ttcccggact ggcagaaaaa
    13021 ttcataagag ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca gctttggcgc
    13081 ttttttggag aacaggaact tatgcagcgc gacggcttcc tccggcggtt ccccttcccc
    13141 ttgcgttgac gtcactacga tgagcagttt ttcgctggcg atttgtttga atttatagtc
    13201 gcccgcgttc accagcttaa cgttcagttt tgctgctaat aaatcatcgc gtaatgcttc
    13261 agcaacccgg cgcgcattgc cggtttgcga ggcggagata atagttatac ccggcatttc
    13321 tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc tgattgagca cgccccagaa
    13381 atagccagaa acccaggcaa gctgggtggg agttaaatcg gtcgtggccg cctgaaggcg
    13441 tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg
    13501 tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat aaaacagaag
    13561 agaaggtaag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg ataactaata
    13621 accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaacct
    13681 aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa
    13741 gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc
    13801 tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac
    13861 cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg
    13921 gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct
    13981 gaactgaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta tctcaatgat
    14041 attccaggtc tggaaaaccc aaccagcgaa gttttagcaa aatggatttg ggatcaggtt
    14101 aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgtac cgcaggttgt
    14161 atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag taacgctatg
    14221 ttagggtgtt gtgttctgga tatctggggc atgacatgga agacgactgc gacattatta
    14281 ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga gcgggtttat
    14341 ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc ggcgggcggt
    14401 tatataccca tgcactcgcg gaactcctcc cgcaatttca cctgaccgcg cctcttgaac
    14461 gacgcatcac tcacgaaagc ctttccctgt taacgccgga tggtgcaacg acgttttcca
    14521 gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat ccgtggctgg
    14581 ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg tgcgacggtg gatgcgctgt
    14641 atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga cgatattctc cgcgcccgtt
    14701 atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg ttagtgactc
    14761 gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg gaaacatccg
    14821 ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg ttcagcggcg
    14881 ggatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa acgctctcgt
    14941 tagggattgt ttgcccactc tcttccctta cgcaaagtcg tgttccggca agcgagctgc
    15001 tggctcgctt taaaacgcat ccggcagtgc gcccgcttat caaaaacacg gaatcactgg
    15061 agtatggtgc gcatctggtg ccagaaggtg gcttgcacag tatgccggtg caatacgccg
    15121 gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg
    15181 tgcgcggcat ggatatggcg ctgactggcg cacaggcggc ggcacaaacg ctgataagcg
    15241 cctgccagca ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac gtcgagcgca
    15301 gcctgctatg ggatattcta cagcgttatc agcatgttcc ggcgcttttg caacgccctg
    15361 gctggtatcg ggcgtggcct gcgttaatgc aggatatttc ccgcgattta tgggatcagg
    15421 gtgataaacc tgttccaccg ctgcgccagt tattctggcg tcatttacgt cgtcatggcc
    15481 tgtggcatct ggcgggcgat gttatcagga gtctgcgatg tctgtagccc ataatctctg
    15541 gcgcgttgct gatgcgccgc acattgttcc ggctgactcc gttgagcgtc agatggtaca
    15601 acggttgatt aacgcctgtc cggcaggtct tttttcgctc acaccggaag gtgacttacg
    15661 cgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg acgaatccac
    15721 actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgct ttggataaat
    15781 aataaggata atttatgccc ctcttacacc tgctccgcca gaatccggtg attgctgccg
    15841 ttaaagataa tgccagcctg caactggcta tcgattctga atgccagttt atttccgtgt
    15901 tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa gattaagaac gccgggaaat
    15961 atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtc gttattcagt
    16021 ttttgaaact ggtgaccgag gcggacggca ttatcagtac caaagcctca atgttgaaag
    16081 cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt tattgttgat tcaatttcgt
    16141 ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag atcctgccag
    16201 gctgtatgcc caaagtgctg ggctgggtga cagagaaaat ccgccaaccg ctgattgccg
    16261 gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt gtcgtggcgc
    16321 tttccaccac gaataccggg gtctggacgt tagcgaaaaa attactttga cgggataatc
    16381 gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc acatccgaca ttcagcgcct
    16441 gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc caggccagaa ccgtaggcag
    16501 gatcatccgg caacacgtta ttggcaatct gcgtgaatgt ttgtcaccag tgcctccagc
    16561 accactttcc agtcatcaac cacgccaaca tctgcctgtg aaaataccgc agcgctggcg
    16621 tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa cgctgctgcg
    16681 ccagaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga aataccaatc
    16741 actttttcag catcaacgcc accgttcatc actcgcgccc gactgtagcc cacttcggct
    16801 cccagctttt ctgccagcat agcgatttcc tggttatctg cctctccgcc ctgccccacc
    16861 accagcaccc gtctggcctc agctaatggg tcgcgagtaa catttttcag atcctctgtg
    16921 ctaaccagcc agtcgggaag cgcacccggg acaatgttca gttgttgcat tccagagggc
    16981 aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg aaaggcatag cgggcgtttt
    17041 tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac gcttacggtt
    17101 ggaatatcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca ggccagccgg
    17161 gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa caacgtccgc aggcgttcgc
    17221 tgccactgtt ccaccaacgc atccagcacc tgttccgcca ctacaggctg aggttcaatc
    17281 tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg ccagccagct tgctattgcg
    17341 gcattctcct gatttattgt cacaattgcg atattcatgg ctgcatcctt tggcgtaaat
    17401 agtcctgcca tagcttttga gctttttctg ctaccgtttg cccatcaatc agcgtcgctc
    17461 cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcatt tcggcagcaa
    17521 cagttttacg gataatttct gctttccccg cagccattcg ctggcgcatt cccggtactg
    17581 gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga acccggcaac
    17641 aacgcaaacc atgttcagta cgctgttcga gggtgataaa cagcgcgtcg agagtgaaac
    17701 gctccacctg ggtgaagcag ggccagccca gcatttccgc cagcaaaaat ggcgtttgcc
    17761 cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga ttctgatgct
    17821 gccattcggc aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct gccgccgtct
    17881 ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag tgcaatgccc
    17941 gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt ctctgcgcca
    18001 acagcagcgc ggcagcggcc tgttcatcag caccgagtaa acttcgcagt agcgaaatat
    18061 ccggtccgct tttaccctga gccgccgcct gccactcttt ttccgccagc attccggcat
    18121 ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt tcccgccgcc
    18181 accagcggga gggctttagt ttctggtgcc catagccagg tcacaacaaa accaaccaac
    18241 aacactgtcg ccaggagcaa taaggtgact tgcattcccc actgcgccag cacccacggc
    18301 agcaggccag tacttaccgc cgcgccaagt cgactcatgg cggtggcaaa accgacgccc
    18361 agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag attactgact
    18421 gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact gccggaaggc
    18481 aaacaggcca ttactaccag cgttgccgcc agcagcaaaa aacttcccag caaaaatttg
    18541 cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg cgcccacaat taacaacgca
    18601 ttaagcatca ggctggcagt cagcgcatct tccagaccaa tagtctgggc gatagttggc
    18661 agccaggtat aaatcacaaa ccatgggatt acgaggcaga caaagaagac gctgttaaac
    18721 gccgtgcgcc gccagtaacg cgaagagaac aaggttttga tgtgtttatg ggtcgccgtt
    18781 accacttcat cgcccagtaa aacatgggga ccaaaatagc gatgcacgat agcgtgagct
    18841 tctgcaaaac gcccctggcg cagtagccag cgtggtgatt ctggcgttcc ccagcgtaat
    18901 aacgtaatca acaacgcggg cagagctgcc gatgccagta gccagcgcca ggcctccggg
    18961 ttttcggaaa taaagtgatg tccggcaata cttgccagca catagcctac ggtccacacc
    19021 acgctgaatg cgcccagcaa aataccgcga tggcggcgcg gggaaaattc agccagcaag
    19081 gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa tcaaaatgcg cagtccaata
    19141 agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag caagcgtaat cagcaaaaag
    19201 ctgaaggtga agattttttg ccgaccaata tggtcggaga tccacccaag aaccaggctg
    19261 ccaaggaaca aaccaaggag cgccgagccg ccgatcattc ccgccataaa cggcgtcagt
    19321 tgcatggcgg gcgtaagctg aataatggcg taaccaatga cgccgagaac ataaccgtcg
    19381 gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt gaaaacggtt aagcggtaaa
    19441 tcatccattc gcaccggtga agtgttcatc tttacccctt cattgtccgg taataagaac
    19501 ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca tcgcggaacg ctgcctgaca
    19561 cattattttt caatcggata gatagtaccc gtattcataa tgccattagg atcgaactgc
    19621 tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct ccagtgaacg
    19681 cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc cgaggcgaat ggtttcttca
    19741 cagatgatct tgttgagcgg attgtggtac ttgtcgattt cctcttccgg cttacagtca
    19801 acgacgttgt aatcgtagac gaagtacatg ttggtgccgt tctgatagct atgagaggaa
    19861 tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact cagtacgaat acggttaata
    19921 acgctttcgt agatttcgtg gatgcagctc cagcagccgg acacttcagt ggtaaagccc
    19981 atgttgccgg ttttgaggat ctgcacacgt tcggcagcca ctttatccgg tccccagttc
    20041 aggttgttaa accaggtttc gatcagtttg ctgtccacgc gctggcactg cgggtagcgg
    20101 gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa tgcgaggatt gccttcagcc
    20161 ataaagatca gcacacattt accgtcggca aaatgggtga agtgttgggt gccatcttca
    20221 gcgtcataca aacgagcgat cgacggacga tacccttcca ccatgacttc acgcaggatg
    20281 ttgaagccgg ttttcatgtc ttccaggata tagccgtaga agaggttgtt ttccggggtg
    20341 aatttaaaga ttttcactgt tacttcagtg atatagcaaa gtgcaccttc gttaccgatg
    20401 atgatgtggc gaatgtccgg gccagccgcg cggcgtggca cgtttttaat gcgtgtgacg
    20461 gtgccatcag ccaatactgc ttccagacca acgaccatat cttcgattgc gccgtagagt
    20521 gtggagaact gcccgatact gcgggttgct accaggccgc ccatctgcgc cagcggcttt
    20581 gactgcggag aatgccccgt ggtgtaacct ttttcacgca acgcattttc cagcacctcc
    20641 agcggaacgc cacattgcgc cgtcgcctgc atattctcaa tatcaatatt aatgatttga
    20701 ttcatggcgg agccgtcgag caccaccgag ttttccacaa cggtttccag cccaccttca
    20761 gtggcggaag cgccggtacg cggcacaccg ttaattttgt gcgcattcat aaaattcagc
    20821 acacgggaga cttgttctgt ggaaccgagt tttacgaccg ctgccggaat cggcaaagta
    20881 taaataccat gaatatccgg aaatttacga aaacggtcaa tactgttctt ctttaatact
    20941 gtttcatcgg taattacgcg atctgcacca acaatttcct ttagctggtc gacaatcgct
    21001 gcgcgagata aagacatagt aattccttcc tgataataaa aatggaataa tcattaataa
    21061 gagaaaacag aaaccaccgt tatggattag cgcactaaat aaccaccatc gaccactaat
    21121 aaatgcccat tgacataatt tgaagccgga cttgcgagga atacagctgc gcccattaaa
    21181 tcctgagtat cgccccaacg gtttgccgga atatgatcaa gaacgcgctg attggtttct
    21241 ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggggc gataccattt
    21301 acctgaatat tatattgacc tagctcatca caataagctt tggtgaaccc ggcaagagca
    21361 tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa caatgaacag
    21421 atattaataa ttttgccgct tttctgcggg atcataattt ttgcagcttc atagcttaac
    21481 tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc agtcggcacg accgaagtcc
    21541 agcaccttat tcagcttaca aataccggca ttgtttacca gaatatcaac tgtaccgaaa
    21601 cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat atccacctgc
    21661 atgaagtcca cctcaacacc ctgtttttca atcatttcct ttgtttcgcc gttatctttg
    21721 acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctgg
    21781 cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc
    21841 attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa
    21901 aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac
    21961 tggagataat ttttagcata acggtataac tgctaatgtg atcgtaatca cagtgtgata
    22021 ttcgtattgt caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg
    22081 ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa
    22141 gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca
    22201 actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg
    22261 acctggctta tttacgttat atttatcaaa ttcccatggc gaaatttatg ggattcagca
    22321 ataccgagat aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc
    22381 ccagcggcgt tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca
    22441 ttaccggatt actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta
    22501 ttcaggtcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg
    22561 cctcgttgct gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc
    22621 gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac
    22681 cggatgacag cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt
    22741 tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg
    22801 aagaaaaaca gtcattccag tttagcgaca tccttgccgt tttgcgtatc agcaccacct
    22861 ggtattgcag catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca
    22921 ccaactatct gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg
    22981 tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg cggcattatc accacctaca
    23041 gtaaagtgaa atcccctacc cgcgtgatcc aaatcctttc cgtactcggc ctgctggcgt
    23101 taactgccct gctcgtcacg aactctaacc cgcaatcggt cgcgatgggg attggcctga
    23161 ttttactgct gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccctggcg
    23221 aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat
    23281 tcctgccgga tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgcag
    23341 cagaagccta ccgcaatatg tggctgatgg gaatggcggc gcttgccatg gtgattgtct
    23401 ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca atggctagca
    23461 gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat
    23521 tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta acgtggtttg
    23581 cgaaggcaaa ggcttattac agccgatgca cacgccagat gccgatactg cagaacatcc
    23641 tgacgacgat ttatgggcat cattatgttt tgccggtcac gatttgatga gtcagtttgc
    23701 cgggaataaa gaagatattg tcggtattgg tctgggatcc atccgttgct gtcgtgcgtt
    23761 attgaaagcc gatggcacgc ttgctgcgcc gttgattagc tggcaggatg cacgcgttac
    23821 acgcccttac gaacacacta atcctgacgt ggcatatgtc acctcttttt cgggttatct
    23881 gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc aactattttg gtcagtggcc
    23941 ggtggattat aagagctgga catggagcga agatgctgcg gtaatggata agtttaatat
    24001 cccccgtcat atgctgtttg atgtgcaaat gcctggcacc gtcctcggac atatcacacc
    24061 acaagccgca ctggcgacac atttcccggc aggactgccg gttgtttgta ccaccagtga
    24121 taaaccggta gaagctctgg gggccggatt actggatgat gaaactgcgg taatttctct
    24181 tggcacttac atcgcattga tgatgaacgg caaagcactg ccgaaagatc cggtggcgta
    24241 ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa
    24301 aggtatgtgg acggtgagct ggctgcgtga catgttaggc gagtcgttaa ttcaggatgc
    24361 cagggcgcag gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc
    24421 tggctgtaat gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa
    24481 acgcgggatt atgatcggct ttgattccag catggattac gcatggatat atcgttcgat
    24541 attggaaagc gtggcgctaa cgctgaagaa caattacgac aatatgtgta atgaaatgaa
    24601 tcactttgcg aagcatgtga tcattactgg cggcggttcg aacagcgatc tgtttatgca
    24661 aatttttgcc gacgtgttca accttccggc acgccgtaac gccattaacg gttgtgcaag
    24721 tctgggagca gcgattaata cagcggtagg tctggggcta tacccggatt acgcaacggc
    24781 tgttgataag atggttcgcg tgaaagatat ctttataccg attgagagca atgccaaacg
    24841 ctacgacgcg atgaataaag gcattttcaa agacctaacc aaacatactg atgtgatcct
    24901 gaaaaaatcg tatgaagtga tgcatgggga attggggaat gtggattcga tccagagctg
    24961 gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc cggcctgaac atacctgaat
    25021 ggttatcccc gctgacgcgg ggaacataag ttcgacaact taaaaaatga taacaagtcc
    25081 ggtttatccc cgctgatgca gggaacataa taaaaacttt aactagttcg gtttatccct
    25141 gtttttacaa ggaatattaa ctacactcga tgtctttaga ataaaagaca atacgaatcg
    25201 cactttatca attttcactt aaagtatgaa ctggatatct ttattcataa gaactattca
    25261 tcaacagcgt atagaggcgg ttatgtaaaa ccactcatta gcctcaaaac aaagacaaac
    25321 ttccagattt attacttaac
//
LOCUS       DAMZII010000071        24614 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZII010000071 DAMZII010000000
VERSION     DAMZII010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.071"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..546
                     /gene="traX"
                     /locus_tag="POA77_002751"
     CDS             <1..546
                     /gene="traX"
                     /locus_tag="POA77_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HDI6366152.1"
                     /translation="VKKSPSITRIATRGIINIFVPLMETRLITNSVQHMLEQQKRRLD
                     RFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCCWSLLAIALFLSGM
                     LFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHERKVSEPEQGTFRD
                     FLNDRNGWRNATLIAVTSKQY"
     gene            643..2805
                     /locus_tag="POA77_002752"
     CDS             643..2805
                     /locus_tag="POA77_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDI6366153.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTQTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTSVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDKGSDDKDGIKG"
     gene            2870..3532
                     /gene="excA"
                     /locus_tag="POA77_002753"
     CDS             2870..3532
                     /gene="excA"
                     /locus_tag="POA77_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDI6366154.1"
                     /translation="MKDKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
     gene            complement(3604..3813)
                     /locus_tag="POA77_002754"
     CDS             complement(3604..3813)
                     /locus_tag="POA77_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDI6366155.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            4205..4381
                     /locus_tag="POA77_002755"
     CDS             4205..4381
                     /locus_tag="POA77_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366156.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(4446..4742)
                     /locus_tag="POA77_002756"
     CDS             complement(4446..4742)
                     /locus_tag="POA77_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366157.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            complement(5365..5517)
                     /locus_tag="POA77_002757"
     CDS             complement(5365..5517)
                     /locus_tag="POA77_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDI6366158.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            5809..7017
                     /gene="trbA"
                     /locus_tag="POA77_002758"
     CDS             5809..7017
                     /gene="trbA"
                     /locus_tag="POA77_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDI6366159.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPYIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            7036..8106
                     /gene="trbB"
                     /locus_tag="POA77_002759"
     CDS             7036..8106
                     /gene="trbB"
                     /locus_tag="POA77_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDI6366160.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            8099..10390
                     /locus_tag="POA77_002760"
     CDS             8099..10390
                     /locus_tag="POA77_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDI6366161.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(10427..13126)
                     /gene="nikB"
                     /locus_tag="POA77_002761"
     CDS             complement(10427..13126)
                     /gene="nikB"
                     /locus_tag="POA77_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HDI6366162.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(13137..13469)
                     /locus_tag="POA77_002762"
     CDS             complement(13137..13469)
                     /locus_tag="POA77_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HDI6366163.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            13702..14037
                     /locus_tag="POA77_002763"
     CDS             13702..14037
                     /locus_tag="POA77_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDI6366164.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(14123..14971)
                     /locus_tag="POA77_002764"
     CDS             complement(14123..14971)
                     /locus_tag="POA77_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6366165.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLSEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            15320..15517
                     /locus_tag="POA77_002765"
     CDS             15320..15517
                     /locus_tag="POA77_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366166.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            15551..15802
                     /locus_tag="POA77_002766"
     CDS             15551..15802
                     /locus_tag="POA77_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6366167.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(15833..16030)
                     /locus_tag="POA77_002767"
     CDS             complement(15833..16030)
                     /locus_tag="POA77_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366168.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEQ"
     gene            complement(16053..16991)
                     /locus_tag="POA77_002768"
     CDS             complement(16053..16991)
                     /locus_tag="POA77_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6366169.1"
                     /translation="MSQKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(17056..17322)
                     /locus_tag="POA77_002769"
     CDS             complement(17056..17322)
                     /locus_tag="POA77_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366170.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(17416..17850)
                     /locus_tag="POA77_002770"
     CDS             complement(17416..17850)
                     /locus_tag="POA77_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDI6366171.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(18579..19079)
                     /locus_tag="POA77_002771"
     CDS             complement(18579..19079)
                     /locus_tag="POA77_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI6366172.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(19541..20137)
                     /locus_tag="POA77_002772"
     CDS             complement(19541..20137)
                     /locus_tag="POA77_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366173.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDDAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(20134..20853)
                     /locus_tag="POA77_002773"
     CDS             complement(20134..20853)
                     /locus_tag="POA77_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDI6366174.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(20850..21284)
                     /gene="psiB"
                     /locus_tag="POA77_002774"
     CDS             complement(20850..21284)
                     /gene="psiB"
                     /locus_tag="POA77_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDI6366175.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(21339..23298)
                     /locus_tag="POA77_002775"
                     /pseudo
     CDS             complement(21339..23298)
                     /locus_tag="POA77_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(23357..23590)
                     /locus_tag="POA77_002776"
     CDS             complement(23357..23590)
                     /locus_tag="POA77_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDI6366176.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(23648..24175)
                     /locus_tag="POA77_002777"
     CDS             complement(23648..24175)
                     /locus_tag="POA77_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6366177.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         5991 a         6658 c         6127 g         5838 t
ORIGIN      
        1 gtcaaaaaat caccatccat aacccgcata gccacccggg ggattattaa catttttgtt
       61 cctctgatgg agactcgcct gataacgaat tctgtacagc atatgctgga acaacaaaaa
      121 cgcagactgg acagatttcg tcaggtaaat aataaaaagg cgcaactcca cctcacgtgg
      181 gaagaagctc tgcaggccag ccatatgtca gtagacgatc tggatcgtcg tttccgccgg
      241 cgcagaacag tatggcgatt ctgttgctgg agtctgctgg ccatcgcttt gtttctcagt
      301 ggtatgctct ttgccgcctc ttcattacct cttacgactc tggtacgcgc catcagcact
      361 cttgtgctga tactgtcagg cgtcgctctt tgtgcttcca gagcactgat tgttacatac
      421 cgcctctggc agttgcatga acgaaaagtc agcgaacccg agcaggggac tttccgtgat
      481 tttctgaacg accgcaatgg ctggcgcaat gccacgttaa ttgccgtaac cagtaaacaa
      541 tactgatcac gtccagatgc cagtaccact ggcatgtcgc catccccctt aattaaatcc
      601 tgtgtggcct ttgcccatac gcaattctat tccggagtta ctgtgaaaat tttacttcgc
      661 gcactctgtg caggccttgc tatatcctca ctgccggcaa tggcttcagt gacctatcag
      721 gatattgtgt ctgccgcaac caatcctgac gatctgtctc gccaggcgct ggtcacgata
      781 ttcggtgatg tagtcaccaa tccactgtca accagcgccc ccactctgat cggaagcatg
      841 ttcggggcgt tcaacagtat catcgccgta cttgccgttg tctggtttat gttcattggt
      901 atccgtcatg ttgtgcgttc ggggcatcag ggacaggtat tcagcaccgg acgcgatatc
      961 gtcggcacac tgagtgtcgt tgcaggtttt ctgatgatcg ttccaaccgg aaatggctgg
     1021 agtatcgctc agctcattat gttatggggc gcatcgataa tgggggttgg gtccgccaat
     1081 gtgatggtgc aacttgccgc tgataatatc gcaaatggtt attcaatgac tgtacagcca
     1141 gttcaggcat ccacacaaac agcagcgcgt ggcatttttg aaatggaact gtgtaagtat
     1201 gcggttaacg caggactaaa tgattttaat cagacggcca aatcctcgac ttcactgatg
     1261 acagaatcag ctaaaaccgc cagtggtaat tacactgtca cggtatccaa tggcagcggt
     1321 atctgtggtt cagccagcct gtcagtagaa ggaaacggaa caacagatca atcgacgatt
     1381 ggaaaattct ttaatccctt cagtaaaaac gaatacagcg gtgttatctc agcacaacgt
     1441 gcagccatgg ataatatgat tagcgacatg gacaacgctg ccagtgagtt tgtgacaaca
     1501 tttctggaaa aaagaaattc tggcaacgga acattgccgg atatcgagac acggattcag
     1561 agagcagccg acgagtatga acgtgctgta caaaaatcgc tacccactga taacggagag
     1621 caatccagaa aagaggctct taaatcttat ctcacaacct acgggtgggt aacgcttggc
     1681 gcatggtatc agacctttgc cactgctaac cagcgtctgg ccgagctggc tgacagagct
     1741 ccagctgtaa cttcaatgtc atcactggga gaagttggtg atactgactt attcagtgca
     1801 gtcatgagtg cctacaagac tcaattacag aacaccacct atacaccacc tctgggcact
     1861 gttacatcag ccaatgagca acgaatggcc aatgcgcaag atcctaagtc tgccttatct
     1921 gaaatgatga gcttctttgt ctatcgaacc aactggatag caacagagat aagtggcaca
     1981 ggaacaacaa gcgatcaggt caatccattg ataaagatga aaaatattgg tgattataca
     2041 ttaggaacaa cagagattct ctggagtgga tatacaacag ttagagttct cgcgacagta
     2101 ggtgataact ctattttcgg aaaggttgcc aatttattct ctggattacc taaaggatta
     2161 gttgctctgc tggatgctgc agctccccca atatatttct tgttgttcct gttgttttgt
     2221 gcgggttttt ccttatcaat ttacctacca tttataccat tcattttctg gatgacaggc
     2281 attgggaact ggatagtcag tgtattaata ggatgtaccg ccggacctct gtgggctgca
     2341 acacatctcg gaacatcaga agatagagga agtcgggcag cctacggtta tatatactta
     2401 attgatagta tgataaggcc gcccatcatg gtctttggtt ttttctttgc ttctgttgcc
     2461 atcatcgccg taggcacaat actgaatgca ttatttggcg ctgccctggt taacgtacag
     2521 ttcaattcgc tgaccggtat tttcagtctt gctggtttct tgctgatata cgccagaatt
     2581 tgtacaacct ctgtagctgc catatttgcc cttcaggcct acctacctga tcatgttatt
     2641 aatttccttg gtgggcgtga gggagccaac actcttggca gcatggcaaa ttctgttaaa
     2701 gaaatattta ttggaggaag tcgcaatatc cgacatactc ctggaatgaa aacagatcgt
     2761 cttaaagata aaggaagtga cgacaaggac ggaataaaag gataatataa atattgcaca
     2821 acctccccat cggcattaaa ataatagata gtacgagggg aggcattgta tgaaagataa
     2881 aaaagagaca cctgacagat ttccaacttg gtggttgtta tattatgtat taaggaaggc
     2941 ttacttcttt ttaggaatac ctttcttttt attttgcgca ctgacatcta cactgatgtt
     3001 atttagttct cgttattatg gtgacaacat tgaagattat gttgtcactt ttggtagttg
     3061 gttcttatta cttgcgccag gtatatggat gtattcacgt gcaaaaacaa ggagagaaaa
     3121 gataagaaaa gttgtacaaa caataaaaga gtcaggcttc tactctccag agaaggggta
     3181 tgaaggatta tctttaactc agggagctta tttcggtatt gaccttaaaa atggcacaat
     3241 gttgtatgtc cgtatctatc caggcaacat tatggatgtt atcggttttg acattcataa
     3301 tttcactcgt actgtaacag acgacaaaac actggaaatt catacgaaat acatcaacct
     3361 gccaatggtt cctattccat catggtgtac tcatccagaa accgcatcaa acaccatgca
     3421 cgctatggca agtcgaggat atgattaccc tgtcgacttt cctcgcctga ttcaggagaa
     3481 gcgtaaggag tgggagcaga ttgcaggcat gcctgtagcc gaagtatttt aagataagct
     3541 ccctccataa cagccggttc aaatgagccg gcttttgttc attacaacag ataacttatg
     3601 ccgttaccga taaattcgac caagtgcttt aatcgcagca atttttattt ccatatcatc
     3661 actttgactc aatctatgta attcacgagt aatgttctcc gcctgacacc ggccttcacc
     3721 taatgcatag atcgcagcaa gtgtaacgtc ttttgattta ctcttcatca tttcaaacaa
     3781 gaaagtatta gctatcgcag gaggcatgct cataatcaat cctcttcatg aaataaacaa
     3841 tggcactcac ttacaaacag gagtccacta tcgcaagcga ctcaacttag caatatctct
     3901 caatttgaat gaaaaaacca gatggcatgt gacagggaac cgtccgcatg atgtccgtga
     3961 ccatttaacc ccataagcta accttgtcac aaacgggcgc agaacagcct gccagacaga
     4021 caacaaaaca aaagtccaag ataaaacatg aataccggta tctcaatgcg aagaaataat
     4081 ctccgtagaa acccccatga gaaaaatatc atatgggaga aaatcgcaat tatcacagcc
     4141 agcgcattca ttattttcgc tgtgctctgg cattaattat tttcaacaca aaaaggaaaa
     4201 aacaatgaat ctggtagatg cctttgtcaa aaaagtaata tccgggccat ataaagaata
     4261 tggaaaatgg tggatagatg ttgaatacat ttcatggggc gttcccggga aaacaagact
     4321 gatgtttgag tcaaaggagc aggcactgga agttaaagaa gggtacaaat ttttaacgtg
     4381 agatctcttt ttgttaaagg gcaggaagcg gtaactccct gcccttagtt acgtttccag
     4441 gcttatcagt cgataagttc ccggatcagt tccagaacag caatcagcgc gtttgcaacg
     4501 gcaactactg ccctgagaac gatgattaac agattaatca tccgcttctc catcactgga
     4561 gaaagaaccc cgttgttcgc tcttacactg cctgtcacaa cggtttctat agtgtcagtt
     4621 atagacagtt cttctccgac cagagcacca aaaccagcac cacctgatgt tttggccagg
     4681 acttgtaacc tgttcttccc gctgaacaat atgccggaca gaacatgcct cagagctgac
     4741 atgccgagta tacacttatc cagtacttgc agaaatataa ttccagacgt actatctaca
     4801 ggtcagttat agacagttct tttccgacca gagccaccca ctctgtaacg gaacagtaaa
     4861 aaaaccgacc tcaccagtcg gtttttttac atctgttgtt tctcaaaatc cgcccgacat
     4921 gcccgtcaca ccggggaaac tcatgctttg aatcagaacc aaccatctca actttgccgt
     4981 ctcctgaaaa aactggcaca ttcagttcga taactaccca taaatacaga gagaaaatcc
     5041 gatgtcagaa aagtggagta aagaattcga ctcttgcttt caggtgggga gcatagaata
     5101 aattgccgat gctctcgaga aacgcagcgg aagcgaggaa aatgcgttag ccatgctggc
     5161 aatgacagcc ttcacactca tgacccgacg tggtatctgc gaaatgcaga acgtggcccc
     5221 ggatggcaaa ggcattcggc tggagctaat cggcttcagg ggaaatgaaa caataccgga
     5281 tacgcttcat tgaagaagaa agggcgcaat gaggtcagcc ttcgcaacaa agcggcggca
     5341 gaaaacctgc cgccgtgttg cccgttaacg tttaacttcg taggctaacg ttgccacaag
     5401 cactgtgttt ccctgctgga gccggagtcc gcaaagcgaa tccctcacca tccagacaaa
     5461 acacagaatc gtcacacaga tgacgattaa catcattaaa aacgttcgct gtggcatcat
     5521 agcccctcca ttgccttacg gcggttatcg ggctactata atgttgtcta ggcattacag
     5581 tagagccctt ggttgattga taaaaaaatc tccaggggct ttctgctgta taccgtccac
     5641 aaaacgcttt cggcatacag cctcaagcgc ccgcagctat tctaacgact ttcgtatctg
     5701 caacaatcct tttatcacac ggctaattcc tctggttagc tcttatcgcc tgcaaataaa
     5761 tttttaacct ttccgtttga tttaaattcc agaaatggag actttgtcat gtcttacaat
     5821 cgacagcctg tcgcagaaga tcctatgcag atatggggcg ctgttggtgt ccttcttatc
     5881 ctgctgctat tcgttatatg gttattcttg ccggaagtgg tttacgccag ctgcctgatt
     5941 cttcatacct tatggggact ggttgactgg ggaccatttc ataactatgc agcaccacga
     6001 tacaacctgc ttgccatgac cggtaacaac gccgccaaca taagctatag ccagtgggtt
     6061 aacgtcatgg agcagaccat aggtattctg tggatgtacc tcttacctgt gacactctgg
     6121 tgtttgtggg agtggtatca acatccggga caaagtcgtt tcacccgcag accagtagat
     6181 attacccgcc tgccatacat ctttgcttcg ttgtcccctg ctatcgcccc tgtactggca
     6241 gatggtgatc cggaaaaact ctttcatggt ggcaaacgac ctgaacggcg ggttgctctc
     6301 accccggaag catttgtcga gcagcatact ctcatcacca atatgcagct cgatgttgca
     6361 gctgcaagac gatgtttcat ggcccagttg ggtaaacctt taacgtcctg gaaagacatg
     6421 gcaccacatg aaaaagcgct ctttgccata tttggtcttc agtatttcct ggatgatcgt
     6481 aaggcagcat taaagctcat ggatacactg aacctctcat gcagaattaa aagcaaacgg
     6541 gacagtggga aattctgtac ccctgtttat tcactggcca aatcagcttt ccagagagtg
     6601 attaaaagca acggagcaca gcaatggctg aaacaacatc gctacgtccg cagtgggctg
     6661 gtctggctgt atgcacatga cctgcggctt acgccaccca actggatttg gctgaaaggc
     6721 gtggacagga cgctgtttta tgcattacac cgcgccaata ccacgaaagg atttattgaa
     6781 ggcgctggcg tggtggccgt tgcacgtgca gaggccgaag ccatgcgttt tggccttccc
     6841 tgccctgaac cctgtgttga tgaagcggtg gaaggcttac ggcgggatat gctcagtctc
     6901 ggtcttatct gggatgaacc acaaccagat cgcgaccgga aacgccgcat cctgaccaac
     6961 tggtccctca ccgacgacat cctgccccgc acacctgcta cagacaacga attctgatta
     7021 tccggagaac tgaaaatgac aatcgagtac tttgcaacac gtattgcaaa acatatttcc
     7081 gtgcaggcaa tctggccgga tggcaggaca gaaattattg ccgtccttcc acgggatgcc
     7141 ctgtcagggc tggtaacacg aaataatcaa ctgattcata tagcctttga cgccatgggc
     7201 acccgtaaca gagaaaccgt cctgattgat aaacaggagc ataacgctga ccagatactt
     7261 acagctattg attcctgcct gcgccttgaa tatctgatgg aacgccgctt cagcagaaca
     7321 cctttgttcc gtggcataat cgccagcgtt gtgctgttcg taatggcaac gattggcttc
     7381 tctctgttcc gctatgttga tcgtgtattc tgggatgata caacgcctga agcagtacag
     7441 acagcaggtg agccacgact gcttccccct cacctgaatc acactgtgcc attaaatgaa
     7501 ggcattcaac tcccggttcc cccgaaaaaa gacgtccagg taccggagaa aacaatcaca
     7561 agcaaaaatc cggaagccgc agctgcgcga cataatctgg ctactgttct gaagcggaac
     7621 gccgatcggg gcatgtttac catcaacctg tcatccggtc atgaacgaac cctgttcgca
     7681 tttctcgatc ctgcctgtcc taactgcaga cttctggagc cggccttaaa acgcctggcc
     7741 agcgacttca atgtcgttat ctacccggtt tcagttattg gtggagagga atccaccgac
     7801 cgtgtggcac cactgctgtg tgaaaaagat gctcagaaac gtgctgccgg ctggcaccga
     7861 ctgtattccg cagataacgg aatgatgacg ccatcagaag aaacaacgcc tgcagatgaa
     7921 acatgcctga aagcagccag agctgcgatt gatgttaaca atgtcgcctt ccgtaagttc
     7981 ggatttgccg gtaccccatg ggtgctgtcc gacaccggct ggcatctgcc tacaggaatt
     8041 ctgcaggaaa ctggcacgct taatttattc ctgaaaacca ccgacagcga gtctggccat
     8101 gagtgaacat cgcgttaacc ccgagctttt acatcgtaca gcctggggaa atcctgtctg
     8161 gaatgcactc cagagtctca atatctatgg cttttgcctg gtggcaagtc ttgtggccag
     8221 ttttatctgg ccacttgcat taccggcatg tctgttgttc acactgataa caatgctggt
     8281 atttagtctg cagcgatggc gctgccctct tcgtatgccc atgacgctgg aatgtgctga
     8341 cccgtcacag gacagaatga ttaaaagaag tcttttcagc ttctggccaa ccctgttcca
     8401 gtatgaggtc attctggaat caccggccag cggcatattc tatgttggtt atcaacgcgt
     8461 cagggatatt ggcagggagt tatggctgag tatggacgat ctgacccgcc atataatgtt
     8521 tttcgccacc accggtggcg gtaaaacgga aaccatcttt gcctgggcga ttaacccact
     8581 ttgctgggca agaggattca cccttgtcga tggcaaggca cagaatgata cggcaagaac
     8641 gatctggtat ctggccagac gctttggacg tgaagacgat gtggaagtca ttaacttcat
     8701 gaacggagga aaatcccgca gtgaaatcat tctgagtggt gaaaaaacgc gtcctcagtc
     8761 aaatacctgg aatcccttct gttacagcac cgaggcattc acagcggaga caatgcagtc
     8821 aatgctgccc cagaacgtcc aggggggcga gtggcagtcc cgggccattg ccatgaataa
     8881 ggcactggta tttggtacta aattctggtg tgtgcgcgaa ggcaaaacaa tgtcgctgca
     8941 gatgctccgt gaacatatga ctctcgaagg gatggccaaa ctttactgtc gtggcctcga
     9001 tgatcagtgg ccggaagaag ctatcgcacc gttacgtaac taccttcagg acgttcccgg
     9061 tttcgacctg tcgctggtca ggacaccatc agcctggacg gaagaaccca gaaaacaaca
     9121 tgcttatctc tccgggcagt tttctgaaac tttcagcaca ttcacggaag cattcgggga
     9181 tatttttgct gaagattccg gtgatatcga tatccgtgac agcattcaca gcgacaggat
     9241 tctgatggta atgatccccg ctctggatac atcagcgcat acaacgtctg ctcttggacg
     9301 aatgtttatc acccagaaaa gtatgattct cgcccgtgat ttgggctacc gcctcgaagg
     9361 aactgattcc gatgcactgg aagtcaaaaa atataagggg cgtttccctt atctgtgttt
     9421 ccttgatgaa gtaggggcat actatacgga ccgtattgct gtggaagcca ctcaggtgcg
     9481 ttcactggat tttgctctga ttttgatggc acaggaccag gaacgtatag aaggacagac
     9541 aacagccacg aatactgcaa cactgatgca gaacacgggg acaaaatttg ccggcaggat
     9601 tgtcagcgag ggtagtacgg ccagaacgct gaaaagcgcc gccggtgaag aagccagggc
     9661 gagaatgaac aacctgcagc gtcaggacgg catatttggt gaatcatgga ttgacagccc
     9721 acaaatcagc atcctgatgg aaagcaagat aaatgttcag gagttgattg aacttcatcc
     9781 tggcgagttc ttttctattt tccggggaga aaccgtacca tcagcatcat tcttcatccc
     9841 tgacgatgag aaaagctgca gcagcgatcc cgttgttata aacaggtata tcagcgtgga
     9901 tgcgccacgc ctggaccgtc tccgccgtct tgttccccgt actactcaac gtcgaatacc
     9961 gtcaccggaa aatgtcagtg ccatcattgg cgtcctgact gcaaaaccct ccagaaaaag
    10021 acgaaaaatt cgcacagagc cgcacacgat cgtggataca tttcagcaac gcattgcagg
    10081 acgtcaggcg gctatggcaa tgctggaaga gtacgacaca gatatcaacg cacgtgaaag
    10141 tgccttgtgg gaaacagcgg tgaatacgct taaaacgacc acacgggaag agcgacgaat
    10201 tcgctatatc acgctgaaca gaccagaact acctgaaaca aaagaggaaa atcagatatc
    10261 agtgagagca gaaagagcag gtataaacct gttaacgctt ccacaggaca ataaccaccc
    10321 gacagggcgt cctgtaaacg ggtttcatca taaaaaaacc aaccgtccgg actgggacgg
    10381 aatgtactga acagtggccg cgccggattc tggcgcggcc ttttttttaa cctggagaag
    10441 gcggttccca gcgtttatcc tgctccggcg gaggcagtga tgcgtcatcg ttgctgttcg
    10501 ccgcgatata atcgcggtag tagcgttcac ttccttcacg gtgatgatcc acgtccggtc
    10561 gcgtaatcct gtagtcttcg tgaccggtac ggcctgtcac attatgccag gcaaccattt
    10621 cagcaaagac ctggttaaat tgtttgtcgt ttccttctgg ttcaaacccc atgcgtggat
    10681 cacgcccgaa taataccggt gcaatcaaat ccagtttatc tgccagcgca ttgcggtcat
    10741 ggtgattacg gaaaacaact ctgtcaccat aatccgtaca gacaaactct cccgtccgac
    10801 ggtcacggaa acgaacactc ccgttcttct gcagacgggc ctcgagatca ccggtattac
    10861 cgtataccgg cgttccgccc ggttcacaaa gcacgacaca gcggtctgtc gtcgttgtgc
    10921 gtgacttgtc tttacggcga tcgcgataat cccagcctct caactgcgat acagcagccc
    10981 tgtctccctg agcagcctga atttcgaccc actgacggta agagggtgga taccatttcc
    11041 cgtcagcaat aagtttctgt cgctcatccc tgatgtcttc tttcagcctg atcaatgcct
    11101 gcattcgctg aacttctgca atgtgataat gcagcttacg taatgccgga tcatcgtact
    11161 gcgcacgaat gtgtgacttc cgcagacggc atgcctgatg aatttcccga caacgttccc
    11221 cataacgcag atccggttta cgccattgtt cacgccatgc cagatagcgg gcacgcaggt
    11281 cagcacgggc ctcagccctg gcttcacgtc gctgccggcg cagcattggg tcacgtttac
    11341 tgctgacccc gtacctgtca ctgacagcca gctccgggtt atagcgattt tcaggttgca
    11401 ccctgtcaaa caaatctgct ggagcactca caaaaggtcc ggcctgtggc tccgcacgcc
    11461 ccagtgtcag atcaggatga atgctgctgg ctttcaccgg cgtttgctca tgattaaaag
    11521 catcaacgac aacaagcccg tgatgttgtt tctgtagcat cagtccctgt tgtgcaaaca
    11581 actcatgaca acgctgccag gtaaactcac cctgtgtgat catttcttca atctgagggc
    11641 tacaatggct gatgctgtat ccccacagac tttctttgtc agaaaacagt tcgcgtttga
    11701 tagccccctg cggccccgta cctgtacgca tacggtgcca ttctttatcg gtcgcaggac
    11761 agctctccat acgacgacca cggaagcgtt ctgttggcgt aacctggcgg aaaatatctg
    11821 ttggtacagg ctgccagcca ccatcgagtt gattcacatt gtcgagcgtc agcagtgacc
    11881 atacgctgtc agcacgaacg ggagtctggt tatgatcata accgtcacag ataaccaggt
    11941 gaccatgctg aacacgcatc cataatcccg cttcagccag tgtgcggtga atagccaggc
    12001 agttttccag ccgtccttcc cgtgccattt ccggcagacg ttctccaaga tgacgacagg
    12061 catactgctg cagactttct gtttcagcga ttttttccgg gcgaacatca gctgcaatga
    12121 agtccggatt atagcgtcct ggtgcttcca cctggctgaa aatgtctttt ggcactggcg
    12181 tgtagtcacc catttttttc atgagttttt ctgcacacca ggagggacca aacgagtcaa
    12241 gctgtacacc ttccctgttg cgatcccagc catccatcac cagaaatttt ccgtccatct
    12301 gagacaggta cagaccatct tccgcaagac gacgatgcag ggataaccag tcatgtacag
    12361 gttcactgcg taaaccagcg accgctgtct gcgcaacata ctcacgaaaa gtttgttttt
    12421 ttccgcgtgt ccaggcgttc tggcgatcac gttcaacggc agttttgcga acgatacgat
    12481 ttcccggcgc atggacccag caaccgttat ccggagcaaa accatgcttc agttcaagct
    12541 cacggcaggc acgactgagt ttttcctgac tccatgataa ccggttcaga taacccgttt
    12601 cagggtgtac ccggttaaca gccacatgga cgtgcagatt atctgtatca gtatgcacgg
    12661 cagagacata ctgatggtcg gcaaggccga gtgatttcag cgtatgacgt acactgtcat
    12721 aaatctgttc cggacgcggg ctttcatgtg actgccagga aaggatgtag tgaaaaacag
    12781 ggtcagtgtc atcttttgca tagtgtgcct gccgggcaat gtactccata tctgcagcgg
    12841 cagtttcaag agaagtacag ttatgaaagc aggtgacacc ataaaagttg acccattcgc
    12901 agccgtcctt catgacatca accagcgcca cgaatgactc atttcgaaga cgtgtcgcat
    12961 aatcaacaag gcgactgaaa cggcttcggt gcggttgttc agcctgcgaa gaagaagata
    13021 aatccagttc ctcatcggtc atgtcatccc ggacagagac gtaggatacc aggtcttcga
    13081 acgaagactt gccgtccctt ctctttttcg gaatgactgc attcaccgga gaatcctcaa
    13141 ccttccatta actgtttacg caatgtattt gtaagttcgg ttatggcaac aagcacttct
    13201 gcgtattcct tgtcaccggt tctttttccc tgaacaaaca gatgcttctg catccttccc
    13261 agtctcatta actccttcag aaaagcatca tctgtacggg agttaattcg cttattcagt
    13321 gctgcatttc ggatgtaagc agatacagta agtccggaat cttcagcatt cttacagata
    13381 gtttcatcct catccggtga gaaccggacc gctctcagga tcgtcttttg ccgtctttca
    13441 ctaccacttc tttgacgttt atcactcacg gtcagctcct tactggggtg tcggggcgaa
    13501 gccctgacca ggaggcaatt gtataatcgc gcgtgcgcgg ttatacaatt gcacatcctg
    13561 tcccgttttt caggccatta tagcctgtta tttttgtgca tcatagtctt atgcagacgg
    13621 cagaggcagg gcgcagccct gccaggagtc agtttttgat gataaaatca gacttcaaca
    13681 tgaaacggag agaaagtgcc aatggcttct aaaaaatttt actcagatga tgacatccag
    13741 ctggcaaaag ctgctttatc ggaattgcca gatctcaccg ctcaacggaa aacattgcgt
    13801 gattttcttg atgccattcg tgatgacatt attgtcctgg taaggacaaa gggttataca
    13861 ctggcagatg ttcgtgacac gctcaaaaat gcagggtatg aagtaggaga gaaggcactt
    13921 cgtgacatta ttcgggaagc agaatcgaaa aaaccatccc gccgttcttc ctctaaaaca
    13981 gcacctaaaa agacagattc aggcaggaaa gatggcatcg atatgaacaa caattgatgt
    14041 gacaatgtca catcgtgttc tgagattatg atgattacag aaaaaaaagc cccggcataa
    14101 ataccagggc ttttctgttt aatcagactc gtggcggcaa tgcacctgga taatgccgtg
    14161 caataatatc attcagtttt tcaacaaggt cagaacgctt aaagacaatg tgtacggtcc
    14221 ctttctggta gccacgcacc gtaaaatatt ccccttctga cagcttaccg acattgtcgc
    14281 cgttcccgag aaattcacca tactgcgccc cgatactgtt cctgaagtca ggaacgttgc
    14341 gcccctccag cagataaaac ggacgtacca ggtcatccag tgcctcccgt ccgctgcagt
    14401 ccagcgtcac acgcccgtta ctccagcggt acagcaggtt ttcaataatg attttcttac
    14461 ccagacggca gggattattg gttctgtaat cccatgacaa tttccggtag acatcaatca
    14521 gtccctgttc aaatgtctgc atcttgcagg cattcagatg gcggaatgtt gccagtacat
    14581 tgtccagggt gatttcagga caggtgtcgc tcatcagctg gctgttccac tcatcacgct
    14641 ggcatgaact catgaaggtg tacatgcccg tatctgtcat caggcggtcc cagattttcc
    14701 ggtcggcaag acgggtgata ttccttttcg cttcttccgg tttatctatg cgacgaacgg
    14761 aatcagtcac gcagtcttca aacccgtaaa gaaaaatatc acctgacgca tcgagcagaa
    14821 tcttgcgcgc tgtcgccagg cattccatcg cctgcatgaa cagtgcaata ccttcgttac
    14881 gctgcgcgat gatgcggtcg atagccacag acggaataac atctgtcgtg ccggcagtat
    14941 tcaggtcagc agtgggtaaa gtctgattca tcacattgtc tcctcatcgt cacgactgcc
    15001 gggacagcca ggtcatttcc cggaggagcg aagctccttc tgccgtccgg gtcttcgggg
    15061 atttccccgc agtggacctt tccgacgggg gacgctcacc acgtcacggg gcagttgtcc
    15121 gggggcagac gcaaaagttt ttgacgcctg cggcaaaaag ttttgtggct ggtacatctc
    15181 ccccctggac gactgaaacg ggatgtggtc atgtatgtac ccccggaaag tccgtctgcg
    15241 gagaacaggc cgcagtgcgg cggcagacag gcagagacag ctgcgccatc aggcgcagtg
    15301 gccttacaca ccgtaaggtg tgcagggagc gccgcatacg gcgcgactga agaaaaacga
    15361 cggcacaaca atggcatgtg gtggtcactg gcacaccact cccgcacagg tgaaaacaga
    15421 gaaaaccgca cgacatttac aagcaaaaaa agtgaaattt cttcactttt ttacacttat
    15481 ttatcgcgct gatttggcaa ttgtgatgat aaaataaagt gaagtttttt cactttttca
    15541 aaggtgtttt atgaccggct gggaactgcg aatctggcga aaaagcatgc tctggtcacg
    15601 cgaaaaagcg gccagagaat ttggcgtgac gcagagaaca tggcacgcct gggaaaatgc
    15661 ggaacaggtg gacgtcaccg tctggaggac cacccaggca ctttcagtcc gtgacctgct
    15721 gcctcacatg caggggatgc gtaaggcaga tatcatcagg cgactggaaa atgaactggg
    15781 ggaaacggca gaggatgtgt gagcgtgatt cacagcctgg ctgaatgcca ggctactgct
    15841 catcaggaaa cagctttttc agttcttcac cggaaagtcc ggttgcctcc tgaatccgtt
    15901 ccggcgtcca gcccatatcc cgcagccggc aggctatttc ccgcgccact tcaagtgcgc
    15961 ctttttgaat aagacgttct gcaatcgtca tgctatttgc tctgctcctg ctgacaaaaa
    16021 agcccggcat tttgccgggc tgtgatgttt actcagtgag tgattttctt cagttcgtca
    16081 tcggaaagtc cggtagcctg cttaatctgc tccggcgtca tccccatttt ctgaagctgg
    16141 cacgcaatag ccagggcttt ttcaagcgct ccttccatgc gtccttccat gcgtccttcc
    16201 atgcgtcctt ccatcctgcc ctcctggcga cctttttgct caagacgttc tgcgatagtc
    16261 atcagtttat cctcatgctg cggcagacgc tcagccagcg cccgcataaa ttccggtgat
    16321 acagagtccc ctgcgttaac gatgtaatga atcagaacac gcacctgctc gtctgaaatt
    16381 tccaccatga cctgcgggag tttgtccagc agctccatca tgtcacgatg gcggatatgc
    16441 ttcatcagca gcgtcagtgc ggccatccgg cgatggttca tgatttcatt gtcatcaacg
    16501 acagtgatat caaccagcgg aaacggcttt gtgtatattt tagccgcaag tgccggattc
    16561 tcaaaacagt ccagccagtt catgctgtac gggtacgggc tgcgctcacc atgatagaac
    16621 agtacaggaa tcaccagtgg caacgttttg tgctgctcca gatgtctctg catcgccgcc
    16681 acactgtaac gtaacatgcg gaaaggcatg tacagatttt cggtgctctg gtgctcgatg
    16741 agcgtataaa catagccatc ttcacccccg gtggttttca cactccagag gatatcgctg
    16801 gcgtactgat gcaggtccgg ctcaacaaag gtggcgggtt ccagcttcag cgtggacagg
    16861 tcgcacaact gccggtactc cgccggaaga tgcagttcca gaaaatctct ggcgacgtcg
    16921 ggattcgcca ggaacgaccg gaacgcggca tcatgcggcg tgggcgtcgt ggtggttttc
    16981 ttctgactca cggttcctcc tggtgagatg tctgaacgta tcctgccgcc tgcacaggca
    17041 gacggcaagg gttaatcagt ggcagacccg ctcaatttca agcgcgcgga gcaggtggcc
    17101 ccattcgctg actttcatag cggggatacg cacccgcttc tgctcactgc agcgtctggc
    17161 gatatggccg ctcatcgcca ggacgtccgg gttaatctca tcctgtgcca gctcctgacg
    17221 tctgaagcgg gtatggcggt gcaggggttt attctcccgc aggcactctt cacgcagacg
    17281 ctttacatac tcggcggttg cgaagataat tgatggtctc atggcataat ctccatagat
    17341 agtgattgag atgccggact tcctggcggg gaagcgccgg cggagagagg cagggtttat
    17401 cgccctgcct ttttttcatt tcggacagcg acgcagtgcc agcgcgagcg ctttcatctt
    17461 ccagccttcc ggcgtgcggt acatcacctt ccagtagccg tcgatgaaca cataatggaa
    17521 gcgcacctct tcagtgcttt cagtggcaat agcctccctg aaagaacggt actcacgcgc
    17581 ctcactgtcc atccggaaac cactgtcacg tccgtaataa gccgtcacac cgtccaccgg
    17641 gttctgaagc gtatgcccgg cgggtttgtc gcaacgggga ccgaggcaac ggatatcacc
    17701 gtgttcaacc agggcttcgg cctgctgacc ggtggcatag tgggtatgca ggatgcgacc
    17761 gttccaggtc ggctccccgt gcaggtggca gtaaacggta cggaacaccc cgtcgcggca
    17821 aaaaacggaa atcgtgctgc tgactgtcat gaaaacgtct ccttctgctg aatcaggtcg
    17881 ccgggccgac cggttgctcc ccggaggggc gaaagcccct tctgccgtcc acttcttcgg
    17941 ggtttctccc cgcagtggac ctttccgatg gggcgtccgt caccacgtca cggggcagct
    18001 gtccgggggc ggacgcaaaa ttttttgccg ggtttatggc taaaaatttt gtggacggta
    18061 tatcttcccc tggactgctg aaacgggatg tggttactcc agcccccgga aagtccacag
    18121 cgggaaacag gccgcagggg tggcggcgga agggttgact gcgccatcag gcgcagtggc
    18181 cttacacacc gtcaggtgtg cagggagcgc cgtttccggc gcgacagggg cggcgcgacc
    18241 ggaacggtcg ctgcaagcct ggtttttcag acatcggacc gtagcggcag cccgaaaccg
    18301 cgccagcggg ttcggcggag actggcgggt gcgccagcag ctgcctgtcg gagtcgggcc
    18361 tgacgaccgg atgccgccag gcagccggga gccgaaggtg tccccgcctc cggaaatcag
    18421 aacaacagaa acgacataac agaaaaatgc ctcacaccat gacgggcagg aagggcagcg
    18481 ccgcaggcgc ggccctcctg catttcaggc aggcgtaagc ctgccagggt tttccggcgg
    18541 cctggcaggc catacccccg ccggagcggg ggagtaaatc accgacggaa cacatgaccg
    18601 tcaataaagg tgaaggagtc caggaataaa tcccgcgcat acgcctcata gtcaaaatag
    18661 agcgccaccg tctccggcac gtcagccagc aggccggtat cactggcgaa ctcaaccgca
    18721 aaagcctcct cactgtcagc ctcgccccac caggcatcgc ggaaggtgtc aaaatccgtc
    18781 tcaccggtat cctccaccca gagacgataa gcctcttcgc agccttcatc ccgtgccagg
    18841 cggaagcctt caacccaggc ccagttgata tggcattcag aggccatatt ccccgggaat
    18901 ccctcataat cctgaaacat cagttcagga tcggcttcat cctggtgaag agcacggcag
    18961 gcggcgaaaa agtcgcgctc atcatcaaac gtggtcaggt caaaccagcg tccggcgatg
    19021 cttccacagt tgtatttgtg ccaggttcca acgtatacag caggtgcaac aacagacatt
    19081 cccgaatcct ccatagtcac atgttctgcg acaccgggcg gtgccgtgcc tccataaaag
    19141 ccgggcgata aggcggggat gcaggggcgc aagggaagcc gcagcctgac gcacacggct
    19201 gacgggagtt gcagggtgag gccgcgaaac ctgagcggac gaacgaccgg aaaatcacgg
    19261 cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc gtacccttgc atccctgcca
    19321 gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcca cagagctgaa
    19381 accccacaag gccatgcccc gcatggccgg gtaaaagcgg agccgccggc gacttcctta
    19441 ttccggcgag tgcagaggac acgccgcatg acctccagac cacccctgat atgaaacgat
    19501 catgatcggc gtgtccctgc gtaaaatata catgcctggt tcaccggaga atatctgtga
    19561 tatccacaag ttttctgtcg cgcagccagt cctgcgcttc acgcaccaca caactgtccg
    19621 gtttcaccgc tttcagcgat gatgccagtc gcttgatcaa catcaccagt tcccgtgtct
    19681 gtatgtccgg cattgtctcc ggcggagcag ggggactctc atttccgacg ctcatcccat
    19741 gcgcttccag tgtggcacgg acaaaccagg cgtcatcgac cagttgctgt ccaatccagg
    19801 ccagatcggc atttctgtca cgggctgcca ttcgctcgcc cgacagcgcc ttgctgatat
    19861 ggctgcgcac cagattatcc aggagtgcct gcgcttcttc cggtgaaaag ccacctggta
    19921 tctctgtgcg caacagccct ttcctgtaca gaagctgcag cagatcaacc tgcgaccgca
    19981 cagcaactat cagtacataa cggacaacat tcgcactggc agtggtttct gtatcattca
    20041 gcagttcttc cggatcgttc cccccggtgt tgcagagcaa cggcaacaga ggtcgcatcc
    20101 acgcgggaac ctggtatttt tcgtctgatt tcatcatatt acgttctcca gcttgttcgg
    20161 gatcatccac tgctcaagtg cacgtacctg taccggtgca ccacgaccgg cctcaccggc
    20221 ttcaaaaatc agccgccaga gtggttcctc ctgccagaaa aaccggtcaa atgatgacag
    20281 tgatggaaaa cgcccgcacc acccccagaa aatcgtttcc agatcatgct cctcaacaac
    20341 accagaccag cgggacaacc agcttccgac cgtggcggga gtatgaaacg caaggtcaat
    20401 ctgcgcctga gcaaaacatg tctggtattc cagttcccgt cgttctgcct cacgttcccg
    20461 ctggcggctg tatttctgct ctgtcagcgt ggttttctga cgacgacgct caccttcacg
    20521 aaaaggcacg gacgggaaca gacggtgccc cgcatcggat ggcaggggaa gaacacagac
    20581 ttccccaaaa ctttcaatca gtttatccac ggcagccagc cacaggagac ggttgccatg
    20641 aaattcctcc ttcgtcagcg caggggcaaa aaagcggaga tcggtcagcg aaatccggcg
    20701 actgccattc aggcagcgaa agaaagcggt ggcataaggc catgctgaaa gtgtgcgtcc
    20761 ctgacgacga cgttgttctg tgacagccac cgcctgcatg gcggcctgtt gttctgcgct
    20821 gagggggatc agtgcccgtg aacgtgcgct catgcctgac ctccttctgc catcaggata
    20881 tggatgatgt cgttaacact gtatccctgc gcatccagtg tcgcggcaag cgccagcgtg
    20941 tgactgatcg cggcaggcgt gaagatatgt tgcacctgaa cgacggcgaa aggctgtcca
    21001 ccaccattca ccagaaccag catccagagc tgagagacgt ctcccggcga acataatgcc
    21061 aggtggaaac gttcgtgggc tggcgtgaaa cgaacctgga cggggaaaaa gcccccgaac
    21121 tcgctgccat actctccgtt catcatccag ttagccggtg cgtccacttt acgcatcacc
    21181 tcgtgagtga gattacggcg catatcgctt cccgcagcac ggatttcttc atattcctgg
    21241 gcattcatgg actgcagggc attcagtgtc agttcggtat tcatcatcgt atttctccct
    21301 ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc agccaggtta
    21361 tcggcgcgtt cagtggcatc cgtctgtgga cgtggtgaac acatccagcc aggcacccag
    21421 cggttgtctt tcatatggaa ctctgcatgt tcagcggcat cgcctttctt catcttctct
    21481 gcgtcccgtg cggcaccgga cagtccggca tcattcaggg ctgcgataat ctgcggcttt
    21541 ttcaggtgtc cgaagaagtt tgctttcgtc ggctgccacc agtcgcgcat gtgaaaaccg
    21601 atggcgctct ccagtgagtc aagcggactg cgtgacgtgc gaccacactc gcgggtctgg
    21661 acaccgttaa ggctgcatgc ggtgcagaaa ctgagcaggg ataacagcac ctcctgactg
    21721 agtgacagga acgtcgtcat gtcccgtgac catccctccg gtaacagggc cgcaagacgg
    21781 gctttttctg ccatcagcgg ccaggaatgc ggcaccttcc ttccctgacg gcgcatcgct
    21841 ggtcagcgaa taatgtttac attccaggct gatttgtgct ggtttactgt acgctccgct
    21901 gtcaaacaca ttcaggcaga gcgtccatgc cagcagtgcc agagatttgt ccggttgctg
    21961 catcagtgct gcctggactg ccagcgtgcg ttcggaagac attttcgtca gcaacggcag
    22021 actgatttcc tccactgacg ctttctcctg cacctgttcc gtgcggtcag cgtcatcagt
    22081 cgcgtcatct tcactgcgca actgcacacc acgctggaca catacgtttc cataacgcca
    22141 ggacaccacc acgccacttc cggcacgcat ctccggcgtc cacgccctga ccttcgccat
    22201 gcagcgcatc agcttcattt ctgcttccag caggtcggat tcctcacact ggttttccag
    22261 cgcgtcgtaa cgcgtcatca gttcgttcag gcgttcttct tctgcctccg tcagcaccgc
    22321 ttccggctcc ggcagacagc ggtatgttcc ggcatcctca cggcactcac cgacaggctc
    22381 catgcgtccg gcgcaccatt cccagccttc ggcttcccga aggtgttcag cgacagcctg
    22441 gagtttttcc agcagggcag catcgagcgc cacgcggtcg acataaccgt ccccgtcatc
    22501 gctgaacaaa tcagtacgca gttcgtcagg tgagaaagca tcagccccca cgaagcggaa
    22561 tttactgtta cctgccaccg ccacttcact ttcggtcacc agacgacgaa tggtctgtac
    22621 ttccggttta ccgccccatc ccgactggca ggcggcttca aacacctgca cctgacgcgc
    22681 ggtgtcgttc tccagcgcca gcgcctgaca gtgctctgtg gtgatgcggt cttctgccag
    22741 cgcatcgagg atgacaggcg caaggtctgc cagtttcagc attcgctgaa cgtggcgggg
    22801 cgaatagccc agcaaatcac cgatttgtgc aggcgttttg ccttcctgcg ccatcgcacg
    22861 gaatccggca atctgttcgg cagggtgcat atcccgacga tgaccgtttt cagtcattga
    22921 tgcagccgtc gccagctcct gcggaataac cttcacgcgt acaggccagt cagccggaat
    22981 gatgccacgc tctgccagca tgttgagtgc agccagtcgg cgaccacctg cggcgacacc
    23041 gtaacggtca ccaggcaggg tatgcacgac cagattctgc agcagtccga cgcccttaat
    23101 ggactccgcc agttcgctga cggactccgc agaatacggc accgtgcgca cattcagcgg
    23161 tgatttaatc agcgaggcca gcggcacgct cacttcctcc gtctgcgcca gcagggcaga
    23221 caggacagtt tcctgcgttt ttacagattt acggcttgct ttgcgggtgg tttctggttc
    23281 acacttcgtt acaggcataa tcatcccctt ataattgctt atgtcctgcc ggacgttcgc
    23341 gcgtccggca cctctcttac ttaagaatcc cgaaatccct gatgtactgg ttcatccagt
    23401 acccggcaca gtcctcaaaa ttccaggagc gccagatcaa cgtgccatcc tgacggacaa
    23461 ccaggcgaaa atgcgttccc tgatcatcct cgataaagac gttccggtac tgtgcggcaa
    23521 cggcttccgc ctgttcgcgg gtaaaggagc cgtccggcag tccctgaagt attctgaaat
    23581 attcgctcat cctcatctct ccggtgatgc cccgcgcagg gcggggcggt tatcacagtc
    23641 aggccagtca gaacgggata tcgtctgaaa acccgtaatc ctccccctcc ggcggttgcg
    23701 gctgaggctc tggctgcgcg gccttacgtc cacggccttt cgttttcgcg ccaccttttt
    23761 tcgtcgcgtc agcactctgt ggctgcccat tctgctgagg cttcggttgc gcctgagcgt
    23821 tctgctgtgg tgcacgtccc agcatctgca cggtgcccgt ggtcttaaca agaatttcag
    23881 tgacgtaacg ggtgataccg ttatcttccc agctacgggt gcgaagctga ccttcgatgt
    23941 agacctgcgc acccttgcgc agatattcac ctgccacttc cgcgagcttg ccgaacagca
    24001 ccacgcggtg ccattccgtc tgctcccgca tctcccccgt ctgtttgtca cgccagcttt
    24061 ctgatgtggc cacctgcagg tttgccactg cgcccccgtt ggggatgtaa cggacttccg
    24121 gatcattgcc cagacgcccg acgaggatga ccttgttgat accacgtgca ctcataagaa
    24181 cctcctgact gaaagatgtg agataaaccg gataccggcg acgggacgtt catgtgcccc
    24241 ttcagaggag cgaaagctcc tcctgccgtc cacttcacag gggtttctcc ccgcagtgga
    24301 cctttccgat gggggcccgt ttaccgcctc acgggacagc tgtccggggg cggacgcaaa
    24361 atttttagcc gggttcatgg ctaaaaattt tgtggccggt gcatctcacc ctggacagat
    24421 ggaacgggag ggggttacgt ctgccccccg gaaagtccac agcgggaaac aggccgcagg
    24481 ggtggtggca ggagggaaga ctgctgcgcc atcaggcgca gtttcctcac acaccgtcag
    24541 gtgtgcaggg agcgcaggtc acggcgcgac aggggcggcg cgaccggaac ggtcgctgca
    24601 agccgggttt tcac
//
LOCUS       DAMZII010000072        24362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZII010000072 DAMZII010000000
VERSION     DAMZII010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.072"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            56..574
                     /locus_tag="POA77_002778"
     CDS             56..574
                     /locus_tag="POA77_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDI6366178.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(619..2664)
                     /gene="dcp"
                     /locus_tag="POA77_002779"
     CDS             complement(619..2664)
                     /gene="dcp"
                     /locus_tag="POA77_002779"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDI6366179.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2801..3547
                     /gene="ydfG"
                     /locus_tag="POA77_002780"
     CDS             2801..3547
                     /gene="ydfG"
                     /locus_tag="POA77_002780"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDI6366180.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3636..4322
                     /gene="ydfH"
                     /locus_tag="POA77_002781"
     CDS             3636..4322
                     /gene="ydfH"
                     /locus_tag="POA77_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDI6366181.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4500..4703
                     /gene="ydfZ"
                     /locus_tag="POA77_002782"
     CDS             4500..4703
                     /gene="ydfZ"
                     /locus_tag="POA77_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDI6366182.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4739..6199)
                     /locus_tag="POA77_002783"
     CDS             complement(4739..6199)
                     /locus_tag="POA77_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI6366183.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6288..7655)
                     /gene="ydfJ"
                     /locus_tag="POA77_002784"
     CDS             complement(6288..7655)
                     /gene="ydfJ"
                     /locus_tag="POA77_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDI6366184.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(7713..8732)
                     /locus_tag="POA77_002785"
     CDS             complement(7713..8732)
                     /locus_tag="POA77_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDI6366185.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8744..9958)
                     /gene="rspA"
                     /locus_tag="POA77_002786"
     CDS             complement(8744..9958)
                     /gene="rspA"
                     /locus_tag="POA77_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDI6366186.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(10164..10490)
                     /locus_tag="POA77_002787"
     CDS             complement(10164..10490)
                     /locus_tag="POA77_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI6366187.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10625..10966
                     /locus_tag="POA77_002788"
     CDS             10625..10966
                     /locus_tag="POA77_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDI6366188.1"
                     /translation="MKITLSKRIGLLAILLSCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            11001..11561
                     /gene="speG"
                     /locus_tag="POA77_002789"
     CDS             11001..11561
                     /gene="speG"
                     /locus_tag="POA77_002789"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDI6366189.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11564..12274)
                     /locus_tag="POA77_002790"
     CDS             complement(11564..12274)
                     /locus_tag="POA77_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDI6366190.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            12382..12687
                     /locus_tag="POA77_002791"
     CDS             12382..12687
                     /locus_tag="POA77_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDI6366191.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12886..15312
                     /locus_tag="POA77_002792"
     CDS             12886..15312
                     /locus_tag="POA77_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDI6366192.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            15373..17796
                     /locus_tag="POA77_002793"
     CDS             15373..17796
                     /locus_tag="POA77_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDI6366193.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17807..18424
                     /gene="dmsB"
                     /locus_tag="POA77_002794"
     CDS             17807..18424
                     /gene="dmsB"
                     /locus_tag="POA77_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI6366194.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            18426..19280
                     /locus_tag="POA77_002795"
     CDS             18426..19280
                     /locus_tag="POA77_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDI6366195.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            19323..19937
                     /gene="dmsD"
                     /locus_tag="POA77_002796"
     CDS             19323..19937
                     /gene="dmsD"
                     /locus_tag="POA77_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDI6366196.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            20096..21388
                     /gene="clcB"
                     /locus_tag="POA77_002797"
     CDS             20096..21388
                     /gene="clcB"
                     /locus_tag="POA77_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDI6366197.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(21341..22036)
                     /gene="bioD"
                     /locus_tag="POA77_002798"
     CDS             complement(21341..22036)
                     /gene="bioD"
                     /locus_tag="POA77_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI6366198.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(22161..23381)
                     /gene="mlc"
                     /locus_tag="POA77_002799"
                     /gene_synonym="dgsA"
     CDS             complement(22161..23381)
                     /gene="mlc"
                     /locus_tag="POA77_002799"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDI6366199.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23516..>24362)
                     /locus_tag="POA77_002800"
     CDS             complement(23516..>24362)
                     /locus_tag="POA77_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6366200.1"
                     /translation="ELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
                     AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRR
                     DYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDH
                     PLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
                     LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHL
                     LNALR"
BASE COUNT         6242 a         6232 c         5864 g         6024 t
ORIGIN      
        1 cgagcataca gaaaaagcaa atcaccttta catataaaag cgtggacaaa aaacagtgaa
       61 cattaatagc gataaaattg tacaacttgc agataccgac actattgaaa acctgacatc
      121 cgcgttgagt caaagactta tcgcggatca attacgctta actaccgccg aatcatgcac
      181 cggcggtaag ttggctagcg ccctgtgtgc agctgaagat acacccaaat tttacggtgc
      241 aggctttgtt actttcaccg atcaggcaaa gatgaaaatc ctcagcgtaa gccagcaatc
      301 tcttgaacga tattctgcgg tgagtgagaa agtggcagca gaaatggcaa ccggtgccat
      361 agagcgtgcg gatgctgatg tcagtattgc cattaccggc tacggcggac cggagggcgg
      421 tgaagatggt acgccagcgg gtaccgtctg gttcgcgtgg catattaaag gccagaccta
      481 tactgcggtt atgcattttg ctggcgactg cgaaacggta ttagctttag cggtgaggtt
      541 tgccctcgcc cagctgctgc aattactgct ataaccaggc tggcctggcg atatctccag
      601 gacagccatt ggtggtgttt atatgttcaa gccacgatgt tgcagcatcg gcataatttg
      661 aggagcctta ccgcgccatt gtcgatacag gcgttccaga tcttcgctgt tacctctgga
      721 aaggatcgcc tcgcgaaaac gctgcccatt ttcacgcgtt aatccgccct gctcaacaaa
      781 ccactggtaa ccatcatcgg ccaacatttg cgtccacaga taagcgtaat aacctgcagc
      841 atatccgcca ccaaaaatat gggcgaaata actgctgcga tagcgtggcg gtatagcagg
      901 aagatccata ttttccgcca ccagcgcccg caattcaaaa tcatcgacat cctgcattgc
      961 ttcgttttct tccaggcaat gccagcgcat atcgagaagt gcggcgctaa gcagttcgct
     1021 catctcatac cctttgttga acaggctggc attacgcatt ttctgttgca gttcgtcagg
     1081 cattgctgcc ccgctctgat aatgccgggc gtagcgagcg aatacctgcg gatgcgttgc
     1141 ccagtgttcg ttgatttgcg acggaaattc gacaaaatca cgcggcgtgt tggtgccgga
     1201 aagcgtggca taacgctggc gggcaaaaag gccgtgcagc gtatgaccaa attcatggaa
     1261 taaggttatt acatcatccc agagtaacaa cgcaggctca ccggcagcgg gtttctgata
     1321 attgcaaacg ttataaatta ccggatgcgt ttcattaagc gttgattgct caacaaaatt
     1381 gcccatccat gcaccgccgc tttttgaatc acgggcgaag aaatcaccgt aaaataacgc
     1441 cagtcccacg ccattatgat caaaaatttc ccacacacga acgtcaggat ggtagacagg
     1501 aatatcaaaa cgttcgacaa acttaatacc gaagagctga ttcgcggtcc agaatacacc
     1561 ttcattcaac accgtgttta attcaaaata tggcttgagc tgcgcctcat caagatcaaa
     1621 tttctcccgc cgtacctgtt cggcataaaa tgcccagtcc cacggctgcg cgctaaaccc
     1681 gccttgctgc ttatcgataa ccgcctgtat ggaggctaat tcatcgctag cacgttgacg
     1741 cgccgctgga acaatttccc gcataaagtt gagtgctgct tcaggtgttt ttgccatctg
     1801 atcggcgatt ttccatgcgg cataatgagg aaaaccaagc agtttcgcct gctgtgcgcg
     1861 aatctctacc agacgttgaa tgatagcgcg ggtatcattg ccatcatttt tttccgctcg
     1921 cgtccagccc gcaataaaca gtttttcacg cgtcgcacga tcgcgcattt cggcaagcgc
     1981 cggttgttgg gtggtattca gcagcggaat cagccatttg ttatccagac ctttctcgcg
     2041 agccgcctct gccgccagcg caatctcttg ctcactcatt cctgccagct gcgcgatatc
     2101 gttcacaacc agaccgccgg atttatttgc tgcaagtaac cgctggttaa actggctggt
     2161 cagggtcgca gcttctgtat tcagtacttt taattttgct ttatcagctt gcgcaagttt
     2221 ggctccggca aggacaaaac gttgatgaat aacctccacc aggcggatgg attcactatc
     2281 aagccccagg gattcacggc gctgccagac agcatctacc cgcgcgaata attcaccgtt
     2341 cagatagata tcattagcca gttccgccag ttcagcggaa aactgttcat caagacgctg
     2401 taattcatca ttggtatgcg ccgcagtcat cgcaaaaaag acgctggtaa cgcgggtaag
     2461 taattctccg ctttgttcca gtgccagaat agtattgttg aaatcaggcg tttgcgggtt
     2521 aagcgcgatg gcagcaattt ctgcccgctt ttgctgcatt ccctcatcga atgccgggcg
     2581 atagtgatga ttggcaattt gatcaaaatg aggagccaga tacggcagtg tgctttgcac
     2641 aaggaaagga ttcattgttg tcattttctt ctcctgaacg cgaggtgttc catagcgtag
     2701 gcttactgat aacggagtgc aatcttgcaa accagtatta cccgctctta agcatcacgt
     2761 gctatgttat tgacacacaa aagcgttgag gaacagtgag atgatcgttt tagtaactgg
     2821 agcaacggca ggttttggtg aatgcattac tcgtcgtttt attcaacaag ggcataaagt
     2881 tatcgccact ggccgtcgcc aggagcggtt gcaggagtta aaagacgaac tgggagataa
     2941 tctgtatatc gcccaactgg acgttcgcaa ccgcgccgct attgaagaga tgctggcatc
     3001 gctccctgcc gagtggtgca atattgatat cctggtaaat aatgccggcc tggcgttggg
     3061 catggagcct gcgcataaag ccagcgttga agactgggaa acgatgattg ataccaacaa
     3121 caaaggcctg gtatatatga cgcgcgccgt cttaccgggt atggttgaac gtaatcatgg
     3181 tcatattatt aacattggct caacggcagg tagctggccg tatgccggtg gtaacgttta
     3241 cggtgcgacg aaagcgtttg ttcgtcagtt tagcctgaat ctgcgtacgg atctgcatgg
     3301 tacggcggtg cgcgtcaccg acatcgaacc gggtctggtg ggtggcaccg agttttccaa
     3361 tgtccgcttt aaaggcgatg acggtaaagc ggaaaaaacc tatcaaaata ccgttgcatt
     3421 gacgccagaa gatgtcagcg aagccgtctg gtgggtgtca acgctgcctg ctcacgtcaa
     3481 tatcaatacc ctggaaatga tgccggttac ccaaagctat gccggactga atgtccaccg
     3541 tcagtaattt ttatacccgg cgtaactgcc gggttattgc ttgtcacaaa aaagtggtag
     3601 actcatgcag ttaactcact cacaagcaag aacgaatgac cgtcgaaacg caacttaatc
     3661 ccacacagcc tgtcaatcag cagatttatc gtattcttcg tcgcgacatt gtccattgcc
     3721 tgattgcacc aggcacaccg ttgtcggaaa aagaagtttc tgttcgtttc aatgtgtcac
     3781 gccagccggt tcgtgaagcc tttattaaac tggcggaaaa cggcctgatt caaattcgtc
     3841 cgcagcgtgg cagctacgtc aacaaaattt ccatggccca ggtgcgaaac ggcagtttta
     3901 tccgtcaggc cattgagtgc gcggtggcgc gtcgggcggc gagcatgatt accgaaagcc
     3961 agtgctacca actggaacaa aatcttcacc agcaacgcat tgccattgag cgcaagcaac
     4021 tggatgattt ttttgaactt gatgacaact tccatcaact cctgacgcag attgccgact
     4081 gtcaactggc gtgggatacc attgagaacc tgaaagcgac cgttgatcgc gtgcgctata
     4141 tgagtttcga ccatgtttct ccaccagaaa tgctgttacg ccagcatctt gatattttct
     4201 ctgccctgca aaaacgtgat ggcgatgcgg tagaacgtgc aatgacgcaa catttgcagg
     4261 aaatcagcga atccgtgcgc cagattcgcc aggaaaacag cgactggttt agcgaagagt
     4321 aattcattta ctctcatccc atccgggatg agaggctttt ccccccacct tacggctcat
     4381 gcatgcatca aaaaagatgt gagcttgatc aaaaacaaaa aatatttcac tcgacaggag
     4441 tatttatatt gcgcccgtta cgtgggcttc gactgtaaat cagaaaggag aaaacaccta
     4501 tgacgaccta cgatcgtaac cgtaacgcaa tcaccactgg cagccgtgtt atggttagcg
     4561 gcaccggtca cactggcaag atcctgtcga ttgatactga aggtctgacc gctgagcaaa
     4621 tccgccgcgg aaaaaccgta gttgttgaag gttgtgaaga gaaactggca cctctggacc
     4681 tgattcgtct cggcatgaac taagtgtgtg aatgccgccg atggcggcat tgcttttttt
     4741 acttcacgga atatttcgcc acggtcgctt tcgcgccatg cgctaataaa gacaagtacg
     4801 cttccgtcac ttttgcagta aacaagctat tgtctggcaa atcatcacca aagattgcct
     4861 taatcgccag caatgactgg acgcgcgctt tcccttcggc actactttgt acagccttct
     4921 gaataacagg taacagtggg tcactgattt ctatcggatt tccctgttca tcaacaccac
     4981 cgacataacg catccaaccc gcgacgccca gcgccagcag atcgaacttg ctgtcatgcg
     5041 ccagatgcca gcgaacagaa tccaacatcc gctgtggcaa tttctggcta ccatccatcg
     5101 caatctgcca ggttcgatga cgtaacgccg ggttgctata gcgtgcaatt aatcggttag
     5161 cgtaatcttg caaatcaacg ccctgcactt tcagcgtcgg cgcttgttcc tgcaacatca
     5221 agccatacgc cgcatgacga taatgttcat cttccataca gtcattaatg tgctgatatc
     5281 cagcaagata acccagatac gccaggaatg aatgactgcc gttgagcatg cgcaacttca
     5341 tctcttcata aggcagtaca tcgctaacca gttctgctcc cgctttttcc cattccggac
     5401 gtccggcaac aaaattatct tctattaccc actggcggaa aggttcacag gcaacgccag
     5461 caggatcgcg cacaccggta agttgttcga ttttcgcaag agtatcctct gtcactgcgg
     5521 gcacaatacg gtccaccatt gttgatggga aagtcacgtt atcttcgatc cattgtgcca
     5581 gttttacatc aacggcttgc gcgtaggaag tgacaacgtc acgcataaca tgaccgtttt
     5641 ctggcatgtt gtcacatgac ataacggtaa atgcgggaag tcctgccgct ttacggcgag
     5701 ccagcgcctc aacaatcacc cctgttgctg ttttcggctg gtggggattt tgcacgtcgg
     5761 cagcgaccat cgggtgatcg agcattaact gtccggtcgc cggagagtgg aaataccctt
     5821 tttcggtgat tgtcagagag acaatcgcaa tttgcggttc acacatcgca gccagcacgg
     5881 tttctaagcc atctatttgt acgtgcaagg cttttttaac gacgccaacg acgcgagccg
     5941 tccacgcgtc ggccgacatt tccgcaacgg tataaagatt atcttgctgt tgtaaatcgg
     6001 caatttgctg ttcgccgccg attaagttga cctcataata tccccagtca ctgaaatgtt
     6061 ccgtagcaag aatatcggca tacacgccct gatgcgcacg gtgaaatgca ccaaagccta
     6121 aatgaacaat tcttggggcc aggttattac gatcataaac agggagtgtc gcttttgctg
     6181 ataacaaatt atttcccata acaattcctt aaatataaat atggcaagct atatgttttg
     6241 ttatatgaat aaaaatcccc tctccggtaa gagaagggat taagggttta cagacttctg
     6301 gaaggttgcg cagctcttac aacacgcggt tgatcttccg cagcgtcttc cagcgcactt
     6361 aaatcacggt ctttcacctc tggcattttc agcgcagaga ttaaaccaat cactgaatat
     6421 gccatgatca taatggcgat cggataccag gattccgtca tggtgcagaa aatacccgcc
     6481 aggataggac caaaaccgga agcgataaga ccaccaattt ctttagaaat agccatccgg
     6541 gtaaagcggt ttttacagcc gaacatttct gccatggtaa tgttttccag agcaaataat
     6601 cccagcaccg cacagttatg aatcacaatc agtgcaacca taatggtgct cggggcatag
     6661 cttttatcta caatgataga aagcattggc catgccagca caatcgcgga ggtattcata
     6721 ataatatacg ggatccggcg accaatttta tcggataacc aaccaaggaa cggaatggtc
     6781 ataaagccga gaatcgaact aatcatcaat gcatctgttg gaattgcttt gttaaacaat
     6841 aacgtctgca ctaaatagcc tgcaaggaaa gtctgaatta acccggagtt acccgcctga
     6901 ccaaaacgca gccctgttgc cagccagaag gatttgctct ggaacatgct accagcaggt
     6961 gcaggttttg ctgtgggttg gttgctgtca ttaaccttct caaagaccgg gctttctttc
     7021 agattcatac gtaaccagat agcaaagacc atcacgacaa cgctcgccag gaacggtata
     7081 cgccatcccc acgccagcag ttcctcttta ctgagaatga agaacataaa ggcccagatt
     7141 gccgttgcgc tcaaggttcc gcagttagtt cccatagcta caaatgagga gataattccg
     7201 cgcttacctt ttggtgcata ttccgccagc atcgtaccgg caccggaaat ttccgcacct
     7261 gcacccaacc cctgaataat acgcagcgtc accagcaaga tgggggcaaa aacaccaatc
     7321 tgtgcatagg tcggtaacac accaattaag gtggtacaga tccccatcat ggtgatggta
     7381 ataaagagca cttttttacg ccctattctg tcgcccattt tgccgaaaat aaatgctccg
     7441 acaatacgcg ccacataacc tgcaccgtag gttcccattg ccaaaattaa cgccattgcc
     7501 gttgatgatt caggaaaaaa tatttcatga aacactaacg ctgcgccgag cgaatataac
     7561 tggaaatcca taaattcaag cgcagtgccc agccatcccg atactgctgc tttcaccaaa
     7621 tccttagtgc ttctttcatg tttttctatt gtcataatgg ttatctctaa aaaagaggta
     7681 agatgcgtac tacttactcg ccgttattgg tattattcag aaaaagtgag taagactttg
     7741 cagcaatgtt tttgatcctg ttcaaataaa ctaatggcat cagcaacatg ctggaaatca
     7801 aacgtatggg taattaattt ttctggttta attaaccctt tacttaacca gtcgataaca
     7861 accggaaatt tatttgcatt taagcgtgaa gagaaaatag agagttcttt tccggtaatt
     7921 ccttgctgaa tcacttcaga cggttcactg gagaagccca tcaatacaat acgtgccgct
     7981 ggagaagcca gcgttacggc ctctttcagg atagaaggat gacaagccgc atcgataatt
     8041 aatgtcggct tgatgccttt ttcagcgaaa atctcgccaa gcggtgtctg gctgttatta
     8101 atcgccctgt ctgccccgct ctctttcgct ttttccagtc gttcatcaat gcgatcggca
     8161 acaatcacat ttttaacgtt atagacgcct tttaatacct gaacgatcgt caggccgatt
     8221 ggaccggcac cataaaccag aacggtatca ttttcagtcg gttgaccatg tccggttacg
     8281 ttagccgcaa tggtaaaagg ttcgatcatc accgcatatt gatcggccac tgcttcagga
     8341 attttccacg cattttttgc cggaaccacg gcatattcac tgaaaccacc gtcagcgtgc
     8401 acacctaata cagcaagtgt cgtacaaacg ttcggtttac ctatagagca cggatagcaa
     8461 tgcccacagc tgaccaccgg atcgacagca acacgttcac cgactctggc gctttccacg
     8521 ccgtcaccca ccgcatcaat gacgccaaag aattcatgtc caatgacgcg cggatatttc
     8581 gcaaaaggat tatgcccacg ataaatatgg ctatcagaac cacaaattcc ggcaagtttc
     8641 acttttactc gtacttcacc cgctgacggg gtgggtattt cacgttcgac aatcgccagt
     8701 tgattcggtt tttcaattaa tatgcttttc attatcttac tccttaccag ttccacagcg
     8761 tgccatcttc cagacgtgcg actggtagat aagcaggttc atagggatat ttcgccgcca
     8821 gcttttcatc gaattcgatg ccaagacccg gtttgtctcc cggatgcata tagccgttat
     8881 cgaaagtcca gttgtgcggg aagacttcga gcatttgttc ggaataaccc atgtattcct
     8941 ggacaccgaa attggggacc cacagatcaa agtgcagcgc tgcagccatg cagactggtg
     9001 acaaatcgga aggaccgtgt gagccagtac gtacctgata cagcgaagca aaatcggcaa
     9061 tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata taatcgatga
     9121 gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca cccactgcga
     9181 tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttct gcaggcgtcg
     9241 ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc gccgcttcaa
     9301 taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca aacttgttac
     9361 gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac cacagctgct
     9421 cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct ttcgacatgc
     9481 cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg aatcccagct
     9541 cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg gtatggcaat
     9601 aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc atgttggcag
     9661 ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac atcgtaaccg
     9721 gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg atacggtgcg
     9781 catcgcgacc aataagctgc ggacaaaggt gatcctgcaa ataagaggcc acggaaagct
     9841 cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg gtgattttta
     9901 atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctttacg atcttcatgt
     9961 tcgattcctt gcatcgcttg tcgtgatgca tgaaatctac gcaactgagc tactaccata
    10021 caagtataaa gatcgaaaaa agccggagtg atcacaaaaa aaggcgtata tttgcgctgt
    10081 gaatggttga caaaagatga aatagaatac cttttgtcag ctgacacttc ctcttatctt
    10141 attgataaaa tggatttatg ttcctacgtg cgcccccagc ccgcaacaat gatcaacatg
    10201 ccgcaaagcg caatcaacgc acccgtccag tcataaagag tcagtttcac gccatccaca
    10261 acgcgcagcc acatcaacgc cgtgcagaca taaacgccac cataagccgc gtaaacacgc
    10321 ccactcgccg ctggatgcaa cgttaacaac cagacaaaca gcgccagtga aatccccgcc
    10381 ggaagcaaca gccagatact ggcgtttcgt tttaaccaca accagggcag aaagcatcca
    10441 ataatttcac acagcgcagt agcaaaaaat agtaacgttg ttttaatcat ctttgtctct
    10501 tattgacatc atgtatagtt atagggcgac ataatatcat caatataaac accctcctgg
    10561 tacgttttgc gtccgcagtg gatggtgtag aatcaccttt aatcattcat acagggaatg
    10621 aattatgaaa atcactctca gcaaacgaat cggcctgctc gctattctgc tgtcttgcgc
    10681 actggcattg agcacaactg ttcatgccga aactaacaaa ctggtgattg agtctggcga
    10741 cagtgcacaa agccgccagc acgccgctat ggaaaaagag caatggaatg acacgcgcaa
    10801 tctgcgccag aaagtgaata aacgcactga aaaagagtgg gataaagccg acgccgcttt
    10861 tgataaccgc gataaatgtg agcaaagcgc caacatcaat gcctactggg agcccaatac
    10921 tttgcgctgc ctggaccgtc gaactggccg cgttattacc ccctaacctg ttattgattt
    10981 aaggaatgta aggacacgtt atgccaagcg cccacagtgt taagctacgc ccgctggagc
    11041 gtgaagattt acgctatgta catcaactcg acaacaacgc cagtgtgatg cgttactggt
    11101 ttgaggaacc ctacgaagcc tttgttgaac tctctgatct gtatgataag catattcacg
    11161 atcagagcga acggcgcttt gtggtggaat gtgacggcga aaaagccggt ctggtggagc
    11221 tggtggaaat taaccatgtt catcgccgcg cagaatttca gataattatc tccccggagt
    11281 atcaggggaa aggtctggca acccgtgccg ccaaattagc aatggactat ggctttaccg
    11341 ttctcaatct ctataagctg tatctgatcg ttgataaaga gaatgaaaaa gcgattcaca
    11401 tttaccgcaa gcttggcttt tcggttgaag gtgaattgat gcacgagttc tttattaatg
    11461 gtcaatatcg taatgctatt cgcatgtgta tattccagca tcagtatctg gcagagcaca
    11521 aaacaccggg tcagactctc ctgaagccga ccgcacaata gcattaataa taatcgatcg
    11581 tatttttgat ggtgtaaacc cgttcgacgg cgggttttac tccttcatca acaatgatta
    11641 gctgacagtc caccggatta gcgtgactgt catattcaca gctctgtttt acattaccaa
    11701 cccgttgatt attcagtaaa gtaaccgctg tgtaatctaa ttttttgatc ggatccgttg
    11761 atggcgtggc gctgacagat aatgttttgt cgttactttt cgtggtttta cccagcggat
    11821 aaccctgatc atcatagcga tattccatct gcatttgttt gctgctggct ttaatcacga
    11881 agccattatc atcggtttcc caactcaccc cggcagaagg taattctgct agctggcatt
    11941 ttccctgtaa acgtactctc ttctccagcg tctcggcatc acggtaataa ttggcgtcca
    12001 gtaccagagc gaccacggta ttattttcca gatccagtaa ttcgagtgaa tcaaaacagc
    12061 cttcttccga caaagtccca gaaacacgtt tcgtcacttc accttgctca tccattaatg
    12121 tctgagtgaa atcttttacc ggaccacgca gcggatcaaa atcgaattca ttagagaaac
    12181 tggccatctc aggggtaaat gaaagtgtta cctctgtgcg gtcacatcct gtgaggaata
    12241 tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt caccagagag tagagatgat
    12301 tctcaatcat agtagcaaat acagtacttt acacgttaaa tgctatgctt aaagaagtta
    12361 tcttcgcgta aggagcttat gatgaaactc tcaacctgct gtgccgcgct tctgctcgcc
    12421 ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg agcgcataca gagcgatata
    12481 tcacagcgca ttatcaataa tggcgtaccc gaaagcagct tcacgttaag tattgtaccc
    12541 aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc attgtgctaa tgatacgcat
    12601 aaaattctct acacccgcac caccagcggc aacgtctctg ctcccgcgca gtctacccag
    12661 gatggcgcgc ctgccgaacc gcagtaatac attatcgact gaacgccgga tacgacacat
    12721 ccggcgattt gaacatacaa cataatccca ccttattact catacccttc tattgatatg
    12781 gattaataat tcttaaccca aaatgggtag actccctcta ttgttagcgc gctaaatatt
    12841 caatatataa acatttatat aacgataaag aacagggagt gagttatgtc caaaaatgac
    12901 cgaatggtgg gcatcagccg cagaacactc gttaaatcaa ccgcgatagg ttctctggcg
    12961 ctggctgcag gcggtttttc tttaccgttc accctgcgca gtgcagcggc agcggtacaa
    13021 caggccagcg aaaaagtgat ctggggtgcc tgttccgtca actgtggtag ccgctgtgca
    13081 cttcgtctac atgttaaaga taatgaagtc acctgggtgg aaactgacaa caccggcagc
    13141 gacgagtatg gcaatcatca agtacgcgcc tgtttgcgtg gtcgatccat ccgccggcgt
    13201 attaatcatc ccgatcgctt gaattaccca atgaaacgcg tgggtacacg cggtgaaggc
    13261 aaattcgaac ggattagctg ggatgaagcc ctggatacta tcgccagtag cctgaaaaaa
    13321 accgtcgaac aatatggcaa tgaggctgta tatattcagt actcttcggg gattgttggc
    13381 ggcaatatga cccgctcttc gccatcagcc tcggcggtca aacgcctgat gaactgctac
    13441 ggcggttcac tcaatcagta tggctcctac agcaccgcgc aaatttcctg tgccatgccc
    13501 tacacctacg gcagtaatga tggcaacagc accacggata ttgaaaacag caagctggtc
    13561 gtgatgtttg gtaacaaccc ggcagaaacc cgcatgagcg gcggtggcat cacttatctt
    13621 cttgaaaaag cgcgcgagaa atcgaacgcc aaaatgattg ttatcgatcc gcgatatacc
    13681 gatacggctg ctggtcgtga agatgaatgg cttcctattc gcccgggcac cgatgccgcg
    13741 ctggttgcgg gtattgcctg ggtattgatt aacgaaaatc tcgttgatca accttttctc
    13801 gataaatact gcgtcggcta tgacgaaaaa accttacccg cagatgcacc caaaaatggt
    13861 cactataaag cctatattct tggtgaaggt gatgataaca cagcgaaaac gccgcagtgg
    13921 gcttcgcaaa ttaccggtat cccggtagac cgtatcatca aactggcgcg tgaaattggc
    13981 acagcaaaac ccgcttatat ctgccagggc tgggggccgc aacgccaggc aaacggcgaa
    14041 ctgactgcac gcgctattgc tatgttacct attttgacgg gcaatgtcgg catcagcggc
    14101 ggaaatagtg gcgcgcgtga atcgacctat accattacca tagaacgcct gccggtgctg
    14161 gataatccgg tcaaaacgtc aatctcctgc ttcagctgga cggatgctat cgatcatggt
    14221 ccgcaaatga cggcaatccg cgacggtgtc cggggcaaag ataaactgga tgtgcccatt
    14281 aagttcatct ggaactacgc gggaaatacc ctggttaatc agcattctga catcaacaaa
    14341 acgcatgaaa ttctgcaaga cgaatcgaaa tgcgaaatga ttgtggtcat cgaaaacttt
    14401 atgacctcat cggcaaaata tgccgacatt ctgctgccag acctgatgac cgttgagcag
    14461 gaagatatta ttcctaacga ctacgccggt aacatgggat atctcatttt cctccagcct
    14521 gtcaccagcg aaaaattcga acgcaaaccg atttactgga tcctgagtga agtcgcgaaa
    14581 cgtcttggac ctgacgtcta tcaaaagttc acagaaggtc gcacgcagga acaatggtta
    14641 caacatctgt acgccaaaat gcttgccaaa gatccggcgt taccgtctta cgacgaactg
    14701 aaaaaaatgg gtatctataa gcgtaaagat cccaatggcc attttgtcgc ctacaaagca
    14761 tttcgtgacg accccgaggc aaatccactt aaaacgcctt ccggtaagat tgaaatttat
    14821 tccagcaggc tggcggaaat tgcccgtacc tgggaactgg aaaaagatga agtgataagc
    14881 ccattgccag tttatgcctc aacctttgaa ggctggaact cccctgaacg tagaaccttc
    14941 ccactgcaac tgtttggttt ccattacaaa tcccgtactc actcgaccta cggcaacatt
    15001 gatgtcctga aagcagcctg ccgtcaggag gtgtggatca accctataga tgcgcagaaa
    15061 cgtgggattg ccaacggcga tatggtgcgg gtgtttaacc atcgtggcga agttcggcta
    15121 ccagccaaag tgacaccacg tattctccct ggagttagcg ctatgggcca gggagcctgg
    15181 cacgaggcca atatgtctgg cgataaaatc gaccatggcg gctgtgtgaa tacgctaacc
    15241 actctgcgcc cttcaccact ggcgaaggga aacccgcagc acactaatct ggtcgagatc
    15301 gaaaaaatat aacccactac aaccataaat tctggcatga cattttgttt gaaaagcaat
    15361 aagtgagtaa tgatgaaaat ccataccaca gaggcgctca tgaaggctga aatcagccgt
    15421 agaagtctga tgaaaacctc cgcacttggc agtctcgcgc tggcaagcag tgctttcact
    15481 ctgccatttt cccaaatggt ccgggcggca gaggctccgg tagaagagaa agcggtctgg
    15541 agttcctgca ccgttaactg cgggagccgc tgtctgttac gtttgcatgt gaaagatgac
    15601 accgtgtact gggtggagtc tgatacgaca ggtgacgacg tctacggtaa tcatcaggtt
    15661 cgagcgtgtt tacgcgggcg ctctatccgc cgacggatga atcatcctga taggttgaaa
    15721 tatcccatga agcgcgtcgg caagcgcggt gaaggtaaat ttgaacggat aagttgggac
    15781 gaagccctgg ataccatcag tgataatctt cggcggatcc tgaaagatta cggcaatgag
    15841 gctgtacatg tcctgtacgg aacaggcgta gatggcggaa acatcaccaa ctcaaacgtc
    15901 ccgtaccgtc tgatgaactc ttgcggtggt tttctcagtc gctatggcag ctacagtacc
    15961 gcacagatca gtgccgcaat gagttatatg ttcggtgcca atgatggcaa cagcccggat
    16021 gatatcgcca atacgaaact ggtcgttatg ttcggaaata acccggcaga aacgcggatg
    16081 agcggcggtg gtgtcactta ctacgtcgag caagcccgcg aacgttcaaa cgcacgcatg
    16141 atcgtcatcg atccacgtta taacgacact gctgccgggc gtgaagatga atggctgccc
    16201 attcgccctg gcaccgatgg cgcactggcc tgtgcgattg cctgggtact gattactgaa
    16261 aacatggtcg atcagccatt tctcgacaaa tattgtgttg gttacgatga aaaaacgctg
    16321 cccgccaacg caccacgtaa cgcgcattat aaagcctata ttctgggcga agggcctgac
    16381 ggcatagcta aaacgccgga atgggcagca aaaatcacca gcatcccggc agaaaaaatt
    16441 atccagttgg cgcgagagat cggttcagca aaacctgctt atatttgtca gggttggggg
    16501 ccacaacgac attccaacgg tgagcaaacg tcccgcgcta ttgccatgct ttccgttctc
    16561 accggtaacg tcggcataaa cggcggcaac tcaggtgtac gcgaaggtag ctgggatctg
    16621 ggggtagaat ggttcccgat gcttgagaat cctgttaaaa cgcagatttc cgtctttaca
    16681 tggacagatg ccatcgacca tggtacggaa atgaccgcga cccgtgatgg tgttcgtgga
    16741 aaagaaaaac tggatgtccc catcaagttt ttatggtgct acgccagtaa cacattgatc
    16801 aatcaacatg gcgacatcaa tcacacccat gaggtgcttc aggatgacag caagtgcgag
    16861 atgattgttg gcattgacca cttcatgacg gcctcggcta agtattgcga tatcctgttg
    16921 cccgacctga tgccaacaga gcaagaagac cttatctctc atgaatctgc agggaatatg
    16981 ggctatgtga tcctcgccca acccgcaacc tcagcaaaat ttgaacgcaa acccatctac
    17041 tggatgctga gtgaagtcgc caaacgctta ggaccagacg tttatcaaac ctttactgaa
    17101 ggtcgcagtc agcatgaatg ggtcaaatat ctccatgcga aaacgaagga acgtaaccct
    17161 gagatgcccg actacgagga gatgaaaacg accgggatct ttaagaaaaa atgcccggaa
    17221 gaacactacg tcgctttccg cgcattccgt gaagatccac aggcaaaccc gttgaaaaca
    17281 ccttcgggga aaatcgaaat ttattctgaa cgactggcga agattgcaga tacctgggaa
    17341 ttgaaaaaag atgaaattat tcatcccctt cctgcgtata ccccaggttt tgatggctgg
    17401 gacgatcccc tgcggaaaac ctatccactg cagttaacgg gcttccatta caaagcgcgt
    17461 acccactcca gctacggcaa tattgatgtg ttacagcagg cctgcccaca agaggtgtgg
    17521 atcaacccca ttgatgctca ggcacgcggt atccgtcatg gcgataccgt gcgggtattt
    17581 aacaataatg gagaaatgct gattgccgca aaagtgactc cgcgtattct gcctggcgtt
    17641 accgccatcg ggcaaggtgc gtggcttaaa gcggatatgt ttggtgaccg ggtcgatcac
    17701 ggcggcagta tcaatattct gacctctcac cgcccttcac cgctggcaaa gggaaacccg
    17761 tcgcacagca atcttgtcca gatcgaaaag gtttaaggag tagcccatga ccacacaata
    17821 tggatttttt atcgattcca gccgctgtac tggctgtaaa acctgcgaac tggcgtgcaa
    17881 agattttaaa gatcttggcc cggaagtcag tttccgccgt atttatgaat acgctggcgg
    17941 cgactggcag gaagataatg gcgtctggca ccagaacgtg ttcgcttatt atctctccat
    18001 ctcctgtaac cattgcgatg accccgcatg tacaaaagtc tgccccagcg gcgcaatgca
    18061 taagcgtgaa gatggctttg tggtggttga tgaagatgtt tgtatcggct gccgctactg
    18121 ccacatggct tgtccgtacg gcgcaccaca gtacaatgct gaaaaagggc acatgacgaa
    18181 gtgcgatggt tgttattcgc gcgtcgccga ggggaaacaa cccatatgcg tcgaatcctg
    18241 cccgctgcgt gcacttgagt tcggtccaat tgaagagctg cgccagaagc acggcactct
    18301 ggctgccgtc gcgccactgc cgcgcgcgca tttcacaaaa cccaatatcg ttatcaaacc
    18361 taacgccaac agccgcccga ccggcgatac cactggttat ctggctaatc cggaggaagt
    18421 gtaaaatggg aaatggatgg catgaatggc cactggttat ctttaccgta ttgggtcaat
    18481 gcgtagtagg cgcgctgatt gttagcggaa ttggctggtt cgccgccaaa aatgacgcag
    18541 accgtcagcg tattgtccgt ggcatgtttt tcctctggct gttgatgggc atcgggttta
    18601 ttgcctctgt catgcacctg ggatcgccac tgcgagcgtt taactcgctg aatcgaatcg
    18661 gagcttctgg tctgagtaac gagattgctg ccggttccat attttttgcc gttggcggac
    18721 tatggtggct ggtggcggtc atcggtaaaa tgccgcaagc gttggggaaa ctctggttac
    18781 ttgtcagcat ggcgctcggc gtcattttcg tctggatgat gacctgcgtg tatcaaatcg
    18841 acaccgtgcc aacctggcat aacggttaca ctacgctggc tttcttcctg acggtattgt
    18901 tgagcggtcc aatacttgct gccgcaattc tgcgggcagc tcgcgttaca tttaatacca
    18961 caccatttgc catcattagc gttctggcgc tgatcgcttg tgccggggtg attgtgctac
    19021 aaggtttgtc tctggcgtgt attcactcat ccgtacaaca agccagcgca ctggtaccag
    19081 attacgcctc cttacaggtc tggcgtgtgg tattgttatg cgcaggtcta ggatgctggg
    19141 tttgtccgct cattcgtcgc cgtgaacctc acgttgccgg acttattctg gggttaatcc
    19201 tcattcttgg cggtgagatg atcgggcgtg tgctctttta tggcctgcac atgacggtag
    19261 gaatggctat tgcaggttaa caaattggtg cggggcaacc cgcacatttc aggatgttag
    19321 gaatgaccca tttttcacag caagataatt tttctgtcgc ggcgcgcgtg ttgggcgcgc
    19381 tgttttatta cgctccagag agtgcggaag ccgcacctct ggttgcggta ctcaccagtg
    19441 acggctggga aactcagtgg cctctaccag aagcgtcatt agcgccgctg gtgacggctt
    19501 ttcagacaca gtgtgaagag actcacgccc aggcctggca gcgtttgttt gtcggcccgt
    19561 gggcactgcc gtctccgccg tgggggtcgg tctggctgga tcgcgaatct gtgctgtttg
    19621 gcgattcaac attggcactt cgtcagtgga tgcgcgagaa aggcattcag tttgaaatga
    19681 agcaaaacga accggaagat cattttggtt cactgctgtt gatggctgcg tggttggcgg
    19741 aaaatggtcg ccagacagaa tgcgaagaat tgctggcatg gcaccttttt ccgtggtcaa
    19801 cacgttttct tgatgttttt atcgaaaaag cagaacaccc tttctaccgt gcactgggtg
    19861 aactggctcg cttaacgctg gcgcaatggc aatcacaact gttaattcct gtcgcggtta
    19921 aaccgctgtt tcgataggat caccgtaata ttgccggatg gtgacgatta cgcgcaatcc
    19981 ggcaataata ggttacagtg tcacgttttt ttatctctta aagcacgcac tgcttttgcg
    20041 gctggcctct tttgccgcaa aatagtcgcc cgtgtttcat tgcccatttc tgctcatgca
    20101 tcatctacac atctatccgg atctgcgcac tatgttccgc cgtctgctta tcgcaacagt
    20161 cgtcggtatt ctcgcggcct ttgccgttgc cgggtttcgt catgcgatgc tgctactgga
    20221 gtggttgttc ctcaataatg actccggcag tctggtcaat gcagcgacaa acctttcccc
    20281 ctggcgacgg ctgctaactc cggcgctcgg cggactggcg gcgggtttgt tgctgatggg
    20341 ctggcagaaa tttacccagc aacgccctca tgcgccgacc gattatatgg aagcgttgca
    20401 aaccgatggt cagtttgatt acgcagcaag cctggttaaa tcgcttgcct ctctgctggt
    20461 agtaaccagc ggcagtgcaa ttggtcgcga aggtgcgatg attcttttag ctgcccttgc
    20521 cgcctcctgt tttgcccaac gttttacacc acgccaggag tggaaattat ggatcgcctg
    20581 tggggccgca gcgggaatgg ctgcggccta tcgcgccccg cttgctggca gtttatttat
    20641 agccgaagtg ctgtttggca ctatgatgtt ggcctctctc ggcccggtga ttatttccgc
    20701 cgtcgtggca ttgctggtta gcaatctgat taatcatagc gacgcgttac tctacagcgt
    20761 acaactctca gtgacggttc aggctcgtga ctatgcgctg attatcagta caggtgtgct
    20821 ggcaggtctg tgcggaccac tgttgttaac gttaatgaac gcctgtcatc gtggatttgt
    20881 gagtctcaaa cttgcgccgc cctggcaact ggcactaggc gggttgatcg tgggtctgct
    20941 ttccctgttc acacctgcag tgtggggcaa cggctatagc accgtacaat cctttttaac
    21001 cgccccacca ctgttaatga tcattgccgg gatcttcctt tgtaaactgt gtgccgtgct
    21061 ggcgagtagc ggttccggcg cacccggtgg tgtctttaca ccgacgctat ttatcggtct
    21121 tgccattggc atgttgtatg gtcgtagcct gggattatgg ttccctgatg gcgaagaaat
    21181 tacgctttta ctcggattga ccgggatggc gacactgttg gcggcaacca cgcacgcgcc
    21241 gattatgtcg acattgatga tatgtgaaat gaccggggag tatcagctac tccccggttt
    21301 attgattgcc tgcgtaattg cgtcggtaat ctcgcggacg ttacaccgtg actctatcta
    21361 ccgccagcac actgcgcagc atagctaagc ggatgtattg ccccagttca cgctgttcgg
    21421 cgcgcggcag ataaggcagt tcaccaatga gcggtgccgg aagtttttta ccgagcacat
    21481 caatgatttc cgcataatgc gccagtcctg ggttgattcg gttagccacc cagccaatga
    21541 gcggcagccc gtcgttggca atcgcctgag ctgttagcag tgcatggtta atgcaacctt
    21601 cctgaatacc gacaaccatc aacaccggca gttgttcctg caccacccat tcagagagtg
    21661 gacgcaaatc attcatcaga ctgcgccagc cgccagtccc ttctaccacg acatgatcga
    21721 ctttttcggt caggtttgcc aggccgtttg aaatgagggt gtaattgatt gggcaactgt
    21781 gcgccacgct actttcttct tcgcttaacg cgataggatt aactgcttca taaggcagtt
    21841 cgatggttga aacactctgc aacaccaggg catctttatt acgcagccct tcgggtgtct
    21901 ctttgctccc cttcgctacg ggtttatatc ccgcaaccgt ttttccctgg gaggctaacg
    21961 cttgtagcaa tgcgcgggaa accaccgttt tccctacaga agtgtctgta ccggtaataa
    22021 agaaacgctt cagcatcact aactccaccg ttatgcttca caaatataaa ccaggaaaat
    22081 aattaacctt gaaagtctaa gttatgcttt cctggcccaa attgagatag cgcaaatttt
    22141 ggtagaacag ttaaaaaatg ttaaccctgc aacagacgaa tcaacaaaga accgttatac
    22201 atcgcgtctt ttaccagtgc agcgcctgcc atcgtgccct ggttagaaaa ctgagtactc
    22261 tcaacgctaa tgtgctgact atacgcagga agggcctgct gacggatgct gtctgagatg
    22321 accgggaaga ggatatctgc cgctttactt aacggtgagc caatcagtat tttttgtggg
    22381 ttaaataaat tcaccatgat ggcaagaatg cgccctacat gcgcgcccac cccggtaatg
    22441 atgtcttttg ccagcagatc gccgcgcaat gctgcctgac acaatgagtc cacggttaac
    22501 ggttgtccat gtaacatcga gctcatggat tgattaagac gcagctgtgc cagctcaaga
    22561 atactgtcca cactggcgat ggtttcgagg cagccgtgat tcccgcaata acagcgtttc
    22621 ccatacgggt cgacctgtgt gtggcctatt tccacgagac tactgctgcc tgcgtgtagc
    22681 agatgaccat cggtaatgac gcccgccccc acgttgtgat cgataaccac ctgaatcaca
    22741 tcacgcgccc cgcgtgaggc accaaacaag gcctctgcca tcgtccatgc gctgatatca
    22801 tgctgaatat aaaccggtac gccggtatgc tgctccagcg cctcgccgag cggcatctct
    22861 tttacatcct catagaacgg catgcgatgt acaataccat tttccgtatc aataattccc
    22921 ggcaaggtta tggcaatcga agttagacgc tcaagttttt tctggtggcg aataaaaaac
    22981 tgatcgatat gggaaatgat acgatccagc aatggcgagt catcttttaa cgccagttcc
    23041 tgcgactctt ccaccaccag tttgctgctc agatcgcgca gagcaaggaa aatctccccg
    23101 cgactaatgc gcagagaaag atagtgccag gcttcagttt caaccaccag ccccaccgcc
    23161 ggacggccac ggttccccgc ttctttgatt tccagctctt gcaccaggtg tgcttcgagc
    23221 atctcacgga caattttagt gatactggca ggagccagct gcgccagacg ggaaagatcg
    23281 atacgcgaga ctggaccaag ctgatcaatc aggcgataaa ccgcgcccgc gttggtctgc
    23341 tttatttgat caatgtgccc aggctggttt tcagcaacca ccgcatactc cctatatttt
    23401 cgcgctccga aataatctgt aggctatggt gaagcacttc aatacgtgtc gtcaaatttt
    23461 tacttaggca tgtgattaac agcacatttt tcgggctttt tcgctgaaat ttccctcacc
    23521 tgagagcatt cagcagatga atacgaaagt tacgcgcagc cggactttgt tcatgatgtt
    23581 tcggccagac caaccacatt tcagaaaccg catcctcttc agcaatcggc acccagcgca
    23641 tttcgttgag ctgaacacgt ttaaatgaag caggcaaaat tgaaacaccc agaccggcgg
    23701 aaaccagacc gatgatggtc attgcctcgc ccacctcctg agtgatgacg ggcgtcaaat
    23761 ggtaacgtcg catcagcccg agaatatcgt catacagccc tgtcccgacg tgcggatcaa
    23821 aaaagacgaa gggttctttc gccagttcag ccagcgttac attcgggtta tttgccaggg
    23881 gatgatcgtg cgggatcatc gccataagcg gttcatggac gatgactgcg tgttcaagcg
    23941 tctccggtaa cgctgtgtta cgcagcaatc ccatatccag cgttccttca atgagcggag
    24001 cgatttgctc acgagtgttc atttcgcggg tttgtaaatg gacatcggga taatcacggc
    24061 gaaacagcga taacgtatcg gacaccgccc gaataaaagg agcggacgaa gtaaaaccaa
    24121 tgcgcaactc ccccgcttcc ccctgatgca gtctttcagc gcgagcggca gcgtcatcta
    24181 ccatagacag gatttgccga ctatctgcaa gaaactgttt tcctgctgcc gtcagcaata
    24241 cactgcgatt agttcgtgcc agcagtcggg caccaatttg ttgctccagt gcctgaatct
    24301 gctgacttag cggtggttgc gaaatattca ggcgggcagc ggcgcgcccg aaatgcagct
    24361 ct
//
LOCUS       DAMZII010000073        24309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZII010000073 DAMZII010000000
VERSION     DAMZII010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.073"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(89..970)
                     /gene="prmA"
                     /locus_tag="POA77_002801"
     CDS             complement(89..970)
                     /gene="prmA"
                     /locus_tag="POA77_002801"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI6366201.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(982..2433)
                     /gene="panF"
                     /locus_tag="POA77_002802"
     CDS             complement(982..2433)
                     /gene="panF"
                     /locus_tag="POA77_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDI6366202.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2423..2665)
                     /locus_tag="POA77_002803"
     CDS             complement(2423..2665)
                     /locus_tag="POA77_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDI6366203.1"
                     /translation="MDTRFVQAHKEAHWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2774..4123)
                     /gene="accC"
                     /locus_tag="POA77_002804"
     CDS             complement(2774..4123)
                     /gene="accC"
                     /locus_tag="POA77_002804"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6366204.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4134..4604)
                     /gene="accB"
                     /locus_tag="POA77_002805"
                     /gene_synonym="fabE"
     CDS             complement(4134..4604)
                     /gene="accB"
                     /locus_tag="POA77_002805"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI6366205.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5582..6556)
                     /locus_tag="POA77_002806"
     CDS             complement(5582..6556)
                     /locus_tag="POA77_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6366206.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6708..8648
                     /gene="csrD"
                     /locus_tag="POA77_002807"
     CDS             6708..8648
                     /gene="csrD"
                     /locus_tag="POA77_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDI6366207.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8953..9996
                     /gene="mreB"
                     /locus_tag="POA77_002808"
     CDS             8953..9996
                     /gene="mreB"
                     /locus_tag="POA77_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDI6366208.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10062..11165
                     /gene="mreC"
                     /locus_tag="POA77_002809"
     CDS             10062..11165
                     /gene="mreC"
                     /locus_tag="POA77_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI6366209.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11165..11653
                     /gene="mreD"
                     /locus_tag="POA77_002810"
     CDS             11165..11653
                     /gene="mreD"
                     /locus_tag="POA77_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI6366210.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11662..12255
                     /gene="yhdE"
                     /locus_tag="POA77_002811"
     CDS             11662..12255
                     /gene="yhdE"
                     /locus_tag="POA77_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDI6366211.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12245..13714
                     /gene="rng"
                     /locus_tag="POA77_002812"
     CDS             12245..13714
                     /gene="rng"
                     /locus_tag="POA77_002812"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDI6366212.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13782..17582
                     /gene="yhdP"
                     /locus_tag="POA77_002813"
     CDS             13782..17582
                     /gene="yhdP"
                     /locus_tag="POA77_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDI6366213.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17738..19183
                     /gene="tldD"
                     /locus_tag="POA77_002814"
     CDS             17738..19183
                     /gene="tldD"
                     /locus_tag="POA77_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDI6366214.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19311..20240)
                     /gene="aaeR"
                     /locus_tag="POA77_002815"
     CDS             complement(19311..20240)
                     /gene="aaeR"
                     /locus_tag="POA77_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDI6366215.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20423..20626
                     /locus_tag="POA77_002816"
     CDS             20423..20626
                     /locus_tag="POA77_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDI6366216.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20634..21566
                     /gene="aaeA"
                     /locus_tag="POA77_002817"
     CDS             20634..21566
                     /gene="aaeA"
                     /locus_tag="POA77_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDI6366217.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21572..23539
                     /gene="aaeB"
                     /locus_tag="POA77_002818"
     CDS             21572..23539
                     /gene="aaeB"
                     /locus_tag="POA77_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDI6366218.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23631..23903
                     /locus_tag="POA77_002819"
     CDS             23631..23903
                     /locus_tag="POA77_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDI6366219.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23959..24222)
                     /gene="yhcN"
                     /locus_tag="POA77_002820"
     CDS             complement(23959..24222)
                     /gene="yhcN"
                     /locus_tag="POA77_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDI6366220.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5767 a         6126 c         6590 g         5826 t
ORIGIN      
        1 caaatttgcg taaataatcg gcaaatttac gtgccttttg tgagcttgct cgcacttcgc
       61 cccgcgtcac cctacggcga tgcgaaggtt aattcttacg accggtaata cggcaccact
      121 cttctttttc cacgaccggg tccagtgcga agctgtcggc ataagcttca caaacgctct
      181 ctgcctggct tgccagaata ccggaaaggc ccagcaaacc gcctgaaacc ggcaggacgc
      241 tgattaacgg tgccagttca cgtaatgggc ctgcaaggat gttagcgacc accacgtcgg
      301 ctttcatttc ttctggctga tctttcggta agtagagttc cagacggtca gaaacgccat
      361 tacgttcggc gttgtcgcgg ctggcctgaa tcgcctgcgg atcgatatca ataccaatgg
      421 cttttgctgc acccagtttc agtgccgcga tcgccagaat gccggaacca cagccaaagt
      481 cgatgactgt tttaccggtt aaatcgaggc tgtcgagcca ttgcaggcac agagaggtgg
      541 ttggatgggt acccgtacca aacgccagcc ctggatctaa catcacgttg acggcgtttt
      601 cgtccggcac atcacgccag ctagggcaga tccacagtcg ttcaccaaag cgcatcgggt
      661 ggaaattatc catccattcg cgctcccagt ctttatcttc tagttgttcg attttatgcg
      721 cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc caccacgtcg ttcatatcgg
      781 tttcagcgtc aaacagacca atcacatcgg tgtcgcccca caggcgcgtt tcgcccggca
      841 gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt gatagaaacg gcacccgctt
      901 ccatcagcgc atcgctaaga tcttccgcgt tcgcgccggt ggtgttcagt ttcagttgga
      961 tccaaggcat ggcaaaactc tttatttatc agtagtcaaa acggtagctt gcgggacgga
     1021 tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt agtagtaacg agggcacgat
     1081 agggtggaag cccaggtact gaatattcag cgtcgcgagt acggcataca gcacgccgcc
     1141 aacgatcatc gcacttagcg cgcctttggc gttggcgcgt tcccagtaaa gacccagcac
     1201 cagcggccac aggaaaacgg cttccagccc accgaaggcc agcaaattca gccagatgat
     1261 catttctggc ggcttccagg cggcaagcag cagcaacgcg ccgagaacta acgtaattac
     1321 cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga tccggacgga tattcagata
     1381 gagatcttta atgatcgtag cggaactttg cagcaactgg gcattgatcg tcgacatgat
     1441 cgcagccata ggtgcagcca ggaagattcc ggcagcaaac ggtggcagca cttttaccat
     1501 taacgtcggg atcaccagat ccggtacggt gagatcgggg atcactgcac gacctaacgc
     1561 tccggccagg tgcataccga acatcaggat tgcgactaca atcgtaccga tgatgatccc
     1621 ccggtgtact gctttgctgt ctttataaga gatacagcgc accgcagtat gcggcaggcc
     1681 aatcacgcca aagcacacca gtacccagaa cgacgtcata aaggcaggcg acagaatatc
     1741 gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt tgcaaggtct gtactgcgtt
     1801 gcttaagccg ccagcggcat gtaccacgcc aataagcagc acaacggtgc caatcagcat
     1861 cacaagccct tgcatggtgt cgttcagcac gctggcgcga aagccaccaa aggcggtata
     1921 taacgcaatg ctgataccaa aaatcagcag cccggtttca taaggaatac ccgccgcggt
     1981 ttccagcagg cgcgcaccgc cgataaactg cacggtcatc gcaccaacga acgcaaccag
     2041 caaactcaaa ctcgccagcc acaccagaag acgactctgg tagcgggcaa acagcatatc
     2101 gttcagcgtc actgcattgt agcggcgcgc aagaatcgca aacttcttgc cgagaatacc
     2161 gagtgaaagc cagactgcag gaagctgaat catcgccagc aatacccagc ccaggccgta
     2221 tttataagca gctcctggcc cgccgataaa cgaactggca ctgatatagg tcgcggtgag
     2281 cgtcatcgcc agcacaatac cgcccataga gcggctgccg aggaaatact cattaaggaa
     2341 ggtgccggtg ctccgtttac gcatcgcata aacagagata ccgaacacca ccaccagata
     2401 ggcgaccagc ggtagaatta cttcaagctg catcgtcatc ctccagtggg atatcgcgat
     2461 agataaattt caccatcgcc cagcacagtc caataaacag tagcggcgtc aggatgcagg
     2521 ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc aacgccaggt aagtaagcgg
     2581 ctactaacca aactgccaga tacaaaaggg tcagccccag cgcccagtgc gcctctttat
     2641 gggcctgaac aaaacgagtg tccatttttt gtccctgatg ggtgaagaaa gcgaggattg
     2701 taccttatgg gggttgtcga tccccagtaa taaaaaaggc cggaaaatcc ggccttttga
     2761 cgcttttgca gtcttatttt tcctgaagac cgagtttttt ctccagatag tggatgttag
     2821 tgccaccatg ctggaagttc tcgtcattca tgatgcggat ctgcagatca acgttggttt
     2881 tgataccgtc gatgatcagc tcctgcagcg cattcttcat gcgagcaatc gccacgtcac
     2941 ggttttcacc gtagcaaatc agcttaccga tcattgagtc atagtacggc ggtacggtgt
     3001 agcccgcgta gatatgagac tcccaacgca ctccaaaacc gccaggtgcg tggaaacggg
     3061 tgattttgcc tgggcttggc aggaaggtgt tcggatcttc ggcgttgata cgacattcca
     3121 ccgcatggcc gcgaacgtga acttcttctt gcttgatcga cagcggttga ccggcagcga
     3181 tacgcagctg ttctttgatc aggtcaacgc cggtgatcat ttctgtaacc gggtgttcta
     3241 cctgaatacg ggtgttcatt tcgatgaaat agaactcgcc gttttcgaac aggaactcga
     3301 aagtacctgc accgcgatag ccgatatcaa cacacgcttt agcacaacgt tcgccgatgt
     3361 agcgacgcag ttccggtgta attcccggtg ctggtgcttc ttcgaccact ttctggtgac
     3421 gacgctgcat agagcagtca cgttccgcca gatagatagc gttgccctga ccgtcagcca
     3481 gtacctgaat ctcgacgtgg cgaggatttt ccaggtattt ctccatgtaa accatatcgt
     3541 tattgaatgc ggctttcgct tccgcacggg tcatggagat ggattgtgcc agttcagcgt
     3601 cgccgcgcac tacacgcata ccacgaccgc cgccgccgcc ggaggctttg ataatcaccg
     3661 gataacctat gcgtttagca atggcacggt ttttatccat atcgtcgccc agcgggccgt
     3721 cagaacctgg tacgcaagga acgcccgctt ttttcatcgc cgcgattgcc gacactttgt
     3781 cacccatcag gcgaatagtg tctgctttcg ggccaatgaa gataaagccg gagcgttcaa
     3841 cctgctcggc aaagttggcg ttctcggaga ggaagccgta acccggatgg attgctactg
     3901 cgccggtgat ttcagcggcg cttatgattg ccgggatgtt cagataactt tttactgacg
     3961 gagcagggcc aatacagacc gtttcatctg ccagtaatac gtgttttaga tcgcgatccg
     4021 cgctggagtg cacagcgaca gtcttgatgc ccagttcttt acaggcacga agaatacgca
     4081 atgcaatctc gccgcggttg gcaataacaa ttttatccag catgttcgcc tcgttactcg
     4141 atgacgacca gcggctcgtc aaattctacc ggttgtccac tttcgaccag aattgctttc
     4201 acggtaccgg atttgtccgc ttcgatctgg ttcatcattt tcatggcttc aacgatgcac
     4261 agggtatcgc ccacgttgac tttctgaccc acttcgatga acgcttttgc gtccgggctt
     4321 ggggtgcggt agaaagtacc aaccatcggg gagcgtacga tgtgaccact gatttccgct
     4381 gctgctggcg cttccatgga aggaacggtc gccggagcgg ctgcgttaga ttgagctggc
     4441 tgctgcatca ttggtgcagc gtaagcttgt tgcatcacag ggaaacttgc ggcaggagct
     4501 gcacggctga tgcgtactga ctcttcgcct tcggagattt ccagttcgga gatgcctgat
     4561 tcttcaacca gctcgatcag ttttttaatc ttacgaatat ccatgagtgg gttccgtact
     4621 ctttgtttag tgtgattgtg acaggcgttt caccgccgtc tgtaaagcgt atgccccgac
     4681 agctgtatgc atagcgataa attccagcag gccgggacgc cggtctattt tgccccagag
     4741 tgtcaatgtt agacttgacg gacattgtgc agcgatctgc tgctatcctc cggcaaaaaa
     4801 caaaatatac cttcgttgcg ccaatgtcac cttttccgca gcgcaaaaac tgtgtcaggg
     4861 aggacgaggt cgcacattat aacgatttcg tagcaattgg cagctaaata ctggtcttat
     4921 cagggaagat aatcaacagc taacatgtaa ataaccttca acaccgtgta atttgcaaca
     4981 agccgcacaa ttcacgaaat tagcgccatc atcgacggaa cttcttataa cgtaaggtga
     5041 aaagctgtaa agccagccct gcgaagatga ggggctgcaa tgagataatc ttcacagacc
     5101 acaaataatg tagggatgcc aggatcgcga caagatagac gacattacgc agcggttgct
     5161 ggggattacc caattttcac tgcatcgtct ggatagatgt gaatgctaaa gcacgcaaaa
     5221 tcaaccgact gacaattctc agcattaaat aggttcgagt ggttaatttg tagtcaaata
     5281 aaatcagatt attcacccca tgggaccaga aatcagtaat aggttaagcg tagtgtcgcc
     5341 caggcaaagc accataaacc taacaggcga cggaggcata tcaataatgg ctgtttagag
     5401 gagtatcaat gcgcatcaat ttgtggtcag tcgaaatcat gactttccag tttggatgat
     5461 gttgtacaac ttcctgacca aggtgttcga cgaaagctgt ccggctaacc gcttttcccg
     5521 ttaaccgatt gaataatgat gtcaaaatga gttccctctc ttattattcc ttgctaaatg
     5581 gttagttaac cttcaccagc gtgcgaccct ggatctggtt attaatgatg gcctcggcaa
     5641 agttcggtgc ctctgacaga gatatctctt ttgccgcctg ggtatagaat gattccggta
     5701 aatcggcgac cagtcgctgc caggcttgtg cgcggcgttc tggtggcgtc attactgaat
     5761 ccaccccttg caaacggaca ttacgcagaa taaatggcat gaccgtggtt ggcagagtaa
     5821 aaccacccgc cagaccacag gccgccacgc agccgccgta attcatttgc gccagcactt
     5881 ttgccagcac tttgtcgcca acagtgtcaa ttgccccagc ccagacttgt ttttccagag
     5941 gacgggattc ggcaaactca tcacgaggga gaatacgact ggcacctaaa cttttcaggt
     6001 attcatgggt actttcgcga ccggaaacgg caacgacctg ataacccaac ttatgcagca
     6061 gcgccacggc ggtactgccg acgccaccac tggcacccgt cacgacaatc tccccgtcct
     6121 gcgggcgaac accggcatct tccagcgcca tcacacacag catggcggta aaaccggcag
     6181 taccgataat cattgcttta cgcgcgtcca gcccttgcgg catggcaacc agccagtcac
     6241 ctttcactcg cgcctgctcc gccagcccac cccagtggtt ttcaccaacg ccccagccag
     6301 tgagtaacac ctcctgaccg gcatgaaaac gcggatcttc gctggtgcgt acagttccgg
     6361 caaaatcgat cccaggaatc atcggaaaat tacggatgat ttttccctta ccggtaatcg
     6421 ccagcgcatc tttatagttc aggctcgacc agtgaacatc aaccgtgaca tcgccctccg
     6481 gcaggcgact ttcgtcaagt gtctgtaccg atgcgagagt tttgccgtcc tgctgttcta
     6541 aaagtaacgc ctgcataagt ggtcctcatg tgcatgatgg attggaaaat aatttctgaa
     6601 gactatactc gctaatggaa aagcaaagcc gatgaagcgc aataaattgc gagataaatc
     6661 tgatttgcta gtatgcccgc ttcctcacta tcggagttaa cacaaggatg agattaacga
     6721 cgaaattttc ggcctttgtt acgctgctca ccgggttaac aatttttgtg actttgctgg
     6781 gctgttcgct aagtttctac aacgcaattc agtataagtt tagtcatcgc gttcaggcgg
     6841 tggcgacggc gattgatacc caccttgtgt cgaatgactt cagcacatta aggccacaaa
     6901 ttaccgaatt aatgatgtcg gcagatatcg ttcgtgtaga cctgctccat ggtgataaac
     6961 aggtttatac cctggccaga aatggtagtt atcgtccggt tggctccagc gatctgtttc
     7021 gcgaactgag tgttccgttg ataaagcatc cggggatgtc gctgcgtctg gtttatcagg
     7081 atccgatggg caactatttc cattctttaa tgactaccgc gccgctcacg ggggcgattg
     7141 gctttatcat tgttatgctc ttcctggcgg tacgctggtt acaacggcaa cttgccgggc
     7201 aagaattgct ggaaacccgg gctactcgta tcttaaacgg tgagcgtggc tctaatgtgt
     7261 tgggaaccat ctatgaatgg ccgcccagaa ccagcagtgc gctagatacg ctgcttcgtg
     7321 aaattcagaa cgcacgcgaa caacacagcc gtcttgatac gctgatccgc tcttatgccg
     7381 cccaggacgt gaaaaccggc ctcaataacc gactcttttt cgataatcag ttagcaacgt
     7441 tactggaaga tcaggagaaa gtaggtaccc acgggatcgt gatgatgatt cgtctgccgg
     7501 atttcaatat gttgagcgat acctgggggc acagccaggt tgaagaacag ttcttcactc
     7561 tgacgaatct gctgtcgaca tttatgatgc gctaccctgg cgcactgctg gcgcgttacc
     7621 accgcagtga ttttgctgcg ctgttaccgc accggacgtt aaaagaggca gagagcatcg
     7681 ccggtcagtt aatcaaagcc gtcgatacct tgccgaacaa taaaatgctc gatcgcgacg
     7741 atatgatcca cattggtatc tgcgcctggc gtagtggtca ggataccgag caggtaatgg
     7801 aacatgcaga gtctgccacg cgtaatgcgg gattgcaggg cggcaatagc tgggctattt
     7861 acgatgactc gttgcctgaa aaaggacgcg gtaatgttcg ctggcgtacg cttatcgagc
     7921 aaatgctcag tcgcggcggc ccgcgccttt atcaaaaacc ggcggttact cgcgaaggtc
     7981 aggttcatca tcgcgaactc atgtgccgca tcttcgatgg taatgaagag gttagctcgg
     8041 cggagtatat gccgatggtc ttgcagtttg gcttatcgga agagtatgac cgtctgcaaa
     8101 tcagccgtct tattccacta ttgcgttact ggccagagga aaatctggcg attcaggtta
     8161 ccgttgagtc gctgattcgc ccgcgttttc agcgttggct gcgcgatacg ttaatgcaat
     8221 gtgaaaaatc acaacgaaaa cgcataatta ttgaacttgc agaggccgat gtaggtcaac
     8281 atatcagtcg tttacaacct gttattcgtt tagtgaatgc tttaggggta cgggtagccg
     8341 tcaaccaggc tggtttgacg ctggtaagca ccagttggat caaagaactt aatgttgagt
     8401 tactcaagct ccatccgggg ctggtcagaa acattgagaa gcgaacggag aaccagctgc
     8461 tggttcaaag cctggtggaa gcctgctccg ggaccagcac ccaagtttac gccaccggtg
     8521 tgcgttcgcg aagcgagtgg cagaccctga ttcagcgcgg tgttacaggc gggcaagggg
     8581 attttttcgc gtcctcacag ccacttgata ctaacgtgaa aaaatattca caaagatact
     8641 cggtttaacc tgccgtttaa tctgttttca cgtagaataa cgcgcgctgc gtctcatggg
     8701 agtgtgcttg tctgctcgcc agattgttgc agcacatatg cagatgaatg accttacgcg
     8761 gttgcaaaca ggcgaggaat gctgctgatg cattaagcct ttctggactc aggcagagat
     8821 ttgtaacaaa ggaaacgaac tgcactaatt ttcaccgtag cagatgattt ttgcgccttg
     8881 tcgctgctgc gtgtggttgg taaagtaagc ggattttctt ttccgcccca gctttcagga
     8941 ttatccctta gtatgttgaa aaaatttcgt ggcatgtttt ccaatgactt gtccattgac
     9001 ctgggtactg cgaataccct catttatgta aaaggacaag gcatcgtatt gaatgagcct
     9061 tccgtggtgg ccattcgtca ggatcgtgcc ggttcaccga aaagcgtagc tgcagtaggt
     9121 catgacgcga agcagatgct gggccgtacg ccgggcaata ttgctgccat tcgcccaatg
     9181 aaagacggcg ttatcgccga cttcttcgtg actgaaaaaa tgctccagca cttcatcaaa
     9241 caagtgcaca gcaacagctt tatgcgtcca agcccgcgcg ttctggtttg tgtaccggtt
     9301 ggcgcgaccc aggttgaacg ccgcgcaatt cgtgaatccg cgcagggcgc tggtgcccgt
     9361 gaagtcttcc tgattgaaga accgatggct gccgcaattg gtgctggcct gccggtttct
     9421 gaagcgaccg gttctatggt ggttgatatc ggtggtggta ccactgaagt tgctgttatc
     9481 tccttgaacg gcgtggttta ctcctcttcc gtgcgcattg gtggtgatcg tttcgacgaa
     9541 gctatcatca actatgtgcg tcgtaattac ggttctctga tcggtgaagc caccgcagaa
     9601 cgtatcaagc acgaaatcgg ttcggcttac ccgggcgatg aagtccgtga aatcgaagtt
     9661 cgtggccgta acctggcaga aggtgttcca cgcggtttta ccctgaactc caacgaaatc
     9721 ctcgaagcac tgcaggaacc gctgaccggt attgtgagcg cggtaatggt tgcactggaa
     9781 cagtgcccgc cggaactggc ttccgacatc tccgagcgcg gcatggtgct caccggtggt
     9841 ggcgcactgc tgcgtaacct tgaccgtttg ttaatggaag aaaccggcat tccagtcgtt
     9901 gttgctgaag acccgctgac ctgtgtggcg cgcggtggcg gcaaagcgct ggaaatgatc
     9961 gacatgcacg gcggcgacct gttcagcgaa gagtaatcgg atgcaggcag gggaaatgtc
    10021 tgtttaccct gcctggtctg atacgagaat acgcataact tatgaagcca atttttagcc
    10081 gtggcccgtc gctacagatt cgccttattc tggcggtgct ggtggcgctc ggcattatta
    10141 ttgccgacag ccgcctgggg acgttcagtc aaatccgtac ttatatggat accgccgtca
    10201 gtcctttcta ctttgtttcc aatgctcctc gtgaattgct ggatggcgta tcgcagacgc
    10261 tggcctcgcg tgaccaacta gaacttgaaa accgggcgtt acgtcaggaa ctgttgctga
    10321 aaaacagtga actgctgatg cttggacaat acaaacagga gaacgcgcgt ctgcgcgagc
    10381 tgctgggttc cccgctgcgt caggatgagc agaaaatggt gactcaggtt atctccacgg
    10441 ttaacgatcc ttatagcgat caagttgtta tcgataaagg tagcgttaat ggcgtttatg
    10501 aaggccagcc ggtcatcagc gacaaaggtg ttgttggtca ggtggtggcc gtcgctaaac
    10561 tgaccagtcg cgtgctgctg atttgtgatg cgacccacgc gctgccaatc caggtgctgc
    10621 gcaacgatat ccgcgtaatt gcagccggta acggttgtac ggatgatttg cagcttgagc
    10681 atctgccagc gaatacggat attcgtgttg gtgatgtgct ggtgacctcc ggtctgggtg
    10741 gtcgtttccc ggaaggctat ccggtcgcgg ttgtctcttc cgtaaaactc gatacccagc
    10801 gcgcttatac tgtgattcag gcgcgtccga ctgcaggact gcaacgtttg cgttatctgc
    10861 tgctgctgtg gggcgcggat cgtaacggcg ctaacccgat gacgccggaa gaggtgcatc
    10921 gtgttgctaa tgaacgtctg atgcagatga tgccgcaggt attgccttcg ccagacgcga
    10981 tggggccaaa gttacctgaa ccggcaacgg ggatcgctca gccgactccg cagcaaccga
    11041 cgacaggaaa tgcagctact gcgcctgctg cgcccacaca gcctgctgct aatcgctctc
    11101 cacaaagggc tacgccgccg caaagtggtg ctcaaccgcc tgcgcgtgcg ccgggagggc
    11161 aatagtggcg agctatcgta gccagggacg ttgggtaatc tggctctctt tcctcattgc
    11221 gctgttgctg caaatcatgc cctggccgga taacctgatt gttttccggc caaactgggt
    11281 gttactcatc ttgttgtatt ggatcctggc cttgcctcat cgcgtaaatg tgggcacagg
    11341 ttttgtgatg ggtgccatac tggatctgat cagcggctcg acgcttggcg tacgcgtatt
    11401 ggcgatgagc atcattgctt acctggtggc gctgaaatac cagcttttcc gcaacctcgc
    11461 attatggcag caggcgctgg tcgtcatgtt gctttcgctg gtggtggata ttattgtttt
    11521 ctgggcagag tttttagtga ttaacgtctc tttcagaccg gaagtgttct ggagtagtgt
    11581 agtcaatggg gtgctctggc cgtggatttt cttgctgatg cgtaaagtcc gtcagcagtt
    11641 tgcagtgcaa taaaggtttc tatgacttct ctgtatttag cttccggttc tccgcgtcgt
    11701 caggagttac ttgcgcaact tggcgtgacc tttgaacgta ttgttacggg cattgaggag
    11761 cagcgtcagc cgcaggagag cgcgcagcag tatgttgtgc gtctggcgcg cgagaaagca
    11821 caggcgggtg tcgcgcaaac ggcgcaggat ctcccggtgc tgggtgcgga tactatcgtt
    11881 atcctgaatg gagaagtgct ggagaaaccg cgcgacgcag agcatgcggc gcagatgttg
    11941 cgcaaattat cgggtcagac ccatcaggtg atgacggcag tggcgttggc cgacagccag
    12001 cacattctcg attgcctggt ggtcaccgat gtgactttca gaacgttaac agacgaagac
    12061 atcgcgggct atgtcgccag cggtgaaccg ttagataaag caggtgcata cggtattcag
    12121 gggctgggtg gctgttttgt caggaagata aatggcagct atcacgccgt agtcggctta
    12181 ccgctggttg aaacgtatga attattaagt aattttaacg cactgcgtga gaaaagggat
    12241 aaacatgacg gctgaattgt tagtaaacgt aacgccttcg gaaacgcgag tggcgtatat
    12301 tgatggcggt attctgcagg aaattcatat tgaacgtgag gcgcgacgcg gaatagtagg
    12361 caatatctac aagggtcgtg taagtcgtgt acttccgggt atgcaggcgg cttttgtaga
    12421 tattgggctg gataaagccg cgtttcttca tgcatccgat atcatgccgc acaccgaatg
    12481 tgtggcgggt gaagaacaaa agcaattcac ggtgcgcgac atctcggaac tggttcgtca
    12541 ggggcaagat ctgatggtgc aggtggtgaa agatccgctt ggcactaagg gggcgcgcct
    12601 gaccaccgat atcacactgc cttctcgcta tctggtcttt atgccagggg cttctcacgt
    12661 tggtgtttcc caacgtattg aaagcgaatc agaacgtgaa cgcctgaaaa aagtggtcgc
    12721 agagtattgc gacgagcagg gcgggtttat catccgtacc gcagcggaag gggttggcga
    12781 ggctgaactg gcctccgatg ccgcttatct gaaacgcgtc tggaccaaag ttatggagcg
    12841 taaaaaacgc ccgcagaccc gttatcagct gtacggcgaa ctggcgctgg cgcagcgtgt
    12901 tctgcgtgat ttcgccgacg ccgaactgga ccgcattcgc gttgactcac gcctgactta
    12961 cgaagcattg ctggagttta cctcggagta cattcccgag atgaccagca agctggagca
    13021 ttacaccgga cgccagccga ttttcgatct ctttgatgtc gaaaacgaaa tccagcgagc
    13081 gctggaacgc aaagtagaac tgaaatccgg tggttatctg attatcgacc agaccgaagc
    13141 gatgaccacc gtggacatca ataccggagc gtttgtcggt catcgcaatc tggacgacac
    13201 cattttcaat accaatattg aagcgacgca ggctatcgct cgccagttac ggttgcgtaa
    13261 tctgggtggg attatcatta ttgatttcat cgatatgaat aatgaagatc accgccgccg
    13321 cgtgctgcac tcgctggagc aggcgttgag caaagaccgg gtgaaaacca gcgttaatgg
    13381 tttttcggcg ctggggctgg tggagatgac gcgtaaacgc acccgcgaaa gcattgagca
    13441 cgtactgtgt aacgaatgcc caacctgcca cggtcgcgga acggtgaaaa ccgtggaaac
    13501 ggtatgctat gaaatcatgc gcgagattgt tcgtgtccac catgcttacg actccgaccg
    13561 tttcctggtc tatgcttctc cggcagtagc tgaagccttg aaaggcgaag agtcacactc
    13621 gctggcggaa gtggaaattt tcgttggtaa acaggttaaa gtacaaattg aaccgctcta
    13681 taaccaggag cagtttgacg tcgtaatgat gtaaacagat gctgggccgc catccggcaa
    13741 agggtttttg agtcacattt ttagcagaca aggagtgacg ggtgaggcga ttgccgggga
    13801 ttttactgct tactggagcc gcgctcgttg tgatcgcagc cctgctggtt agcggcctgc
    13861 gtattgcttt accgcatctt gacgcctggc gtccggaaat cctcaacaaa atagaatccg
    13921 cgactggcat gccggtagaa gccagtcagc tctcagccag ctggcagaat tttggcccga
    13981 cgcttgaagc acacgacatc cgtgcagaac taaaagatgg cggcgaattt tcggttaaac
    14041 gcgttactct ggcgctggat gtctggcaga gcctgttaca tatgcgctgg cagtttcgcg
    14101 acctcacttt ctggcagctg cgctttcgca ccaacactcc tatcaccagc ggtggtagcg
    14161 atgacagtct ggaagccagt cacatcagcg atctgtttct tcgtcaattt gaccatttcg
    14221 atctccgcga cagtgaagtc agttttctga cgccatccgg tcagcgcgcc gagctggcga
    14281 tcccacaact cacctggctg aacgatccac gtcgacaccg tgcggaaggc ctggtaagcc
    14341 tctccagcct taccggacag cacggcgtga tgcaggtgcg catggatttg cgcgatgatg
    14401 aggggttgtt aagcaatggt cgcgtctggc tacaggcgga tgacatcgac ctgaagccgt
    14461 ggctcggtaa atggatgcag gacaatattg ctctggaaac ggcacagttc tcccttgaag
    14521 gctggatgac gatcgacaaa ggcgatgtaa ccggcggtga cgtctggctg aaacagggcg
    14581 gtgccagctg gttgggcgag aaggaaacgc atacgctgtc ggtggataat ctgaccgcgc
    14641 atattacgcg tgaaaatccg ggctggcagt tctctattcc cgatacacgg atcacgatgg
    14701 acggtaaacc ctggccgagc ggagcattga cgctggcctg gataccggaa caggacgttg
    14761 gcggcaaaga caataaacgc agtgacgaac tccggattcg cgccagtaat ctggagctgg
    14821 caggcctgga gggcatacgc ccgctggccg cgaaactttc acctgcactg ggtgatgttt
    14881 ggcgctccac acaaccgagc ggcaagatta acactctggc gctggatatc ccgcttcagg
    14941 cggcagacaa tacccgtttt caggcatcgt ggagcgatct ggcctggaag caatggaaat
    15001 tattaccggg tgcggaacac ttctccggga cgctttccgg cagcgttgaa aatggtttgc
    15061 ttaccgcgtc gatgaagcag gcaaagatgc cttacgaaac ggtattccgt gcgccactgg
    15121 aaatcgccga cggtcaggca actataagct ggctgaacaa tgacaaaggt ttccagctgg
    15181 atgggcgtaa tattgacgtt aaagccaaag ccgtccatgc gcgcggcggt tttcgttacc
    15241 tgcaacctgc taacgatgaa ccctggctgg gtattctggc tggcatcagt accgatgatg
    15301 gttcacaagc ctggcgctat ttcccggaaa acttgatggg taaagacctg gttgattact
    15361 taagtggcgc gattcagggc ggtgaagcgg ataacgcgac gctggtttat ggtggcaatc
    15421 cgcaactctt cccctataaa cacaacgaag gtcagtttga agtgctggtg ccgctgcgca
    15481 acgcgaagtt tgccttccag ccggactggc ctgcattaac taaccttgat attgaactgg
    15541 actttattaa cgacggttta tggatgaaaa ccgatggcgt taatctgggc ggcgtgcgcg
    15601 cgagtaatct caccgcagtg atccctgact actcaaaaga aaaactgctg attgacgctg
    15661 acattaaagg tccgggtaaa gccgtaggcc cttactttga tgagacaccg ctgaaagatt
    15721 ccctgggtgc gaccctgcaa gaactccagc tcgacggcga tgtgaatgct cgcttacatc
    15781 ttgatatccc gctgaacggc gagttggtaa ccgcgaaagg tgaagtgacg ctgcgtaata
    15841 acagtctgtt tatcaaacca ctcgacagca ccctgaaaaa tttgagcggt aaattcagct
    15901 ttatcaatgg cgatctgcaa agtgaaccac tgacagcaag ctggtttaat cagccgttga
    15961 acgtggattt ttccaccaaa gaaggggcaa aagcctacca ggttgcggta aacctcaacg
    16021 gtaactggca accggcgaaa accggcgttc tgcctgaagc ggtgaacgaa gcattgagtg
    16081 gcagcgtggc gtgggatggt aaagtgggca ttgagctgcc ttatcatgct ggcgcgacct
    16141 ataacgtaga gctgaacggc gatctgaaga atgtgagcag tcacttacct tcaccgttag
    16201 ccaaacctgc gggtgaacca ctagcggtaa acgttaaggt tgatggcaat ctcaacagct
    16261 ttgaattaac cggacaggct ggtgcggata accatttcaa tagccgctgg ttgcttggtc
    16321 aaaagctgac gctcgatcgt gctatttggg cggcagacag taaaacgctc ccgccgttgc
    16381 cggaacaaag tggcgttgaa ctcaatatgc cgccgatgaa tggtgccgag tggctggccc
    16441 tgttccagaa aggcgcggcg gagagtgtcg gtggtgcagc gagtttccca caacacataa
    16501 cgttacgtac gcctatgttg tcactgggaa atcagcaatg gaataacctg agtattgttt
    16561 cgcaaccgac ggcaaatggc acccaggttg aggcgcaagg gcgtgaaatc aacgccacgc
    16621 tagcgatgcg taataacgcg ccgtggctgg cgaatatcaa atatctttat tacaacccga
    16681 gcgtggcgaa aactcgtggt gattcaacgc cgtcatcacc tttcccgaca acggagcgca
    16741 ttaacttccg tggctggccg gacgcacaaa tacgatgcac agagtgctgg ttctgggggc
    16801 aaaaattcgg tcgcattgac agtgatctca ccatttctgg cgatacgtta acgctgacca
    16861 atggactgat tgatactggt ttctcgcggc ttactgccga tggtgaatgg gttaataatc
    16921 cggggaatga acgtacctcg ctgaaaggaa aactgcgcgg gcagaaaatt gatgccgccg
    16981 cagaattttt tggtgtcacg acgcccatac gccagtcgtc atttaatgtg gattacgatt
    17041 tacactggcg caaagcaccg tggcagccag atgaggcgac gttgaatggc atcattcata
    17101 ctcaactggg caaaggcgaa attaccgaaa tcaataccgg acatgccggg caattgctgc
    17161 gcttattgag cgtagatgcc ctgatgcgta agctgcgttt tgatttcaga gacacctttg
    17221 gtgaagggtt ctattttgac tccattcgca gcaccgcgtg gattaaagac ggtgttatgc
    17281 acaccgacga cacgctggtg gatggcctgg aagcggacat tgcgatgaaa gggtcggtaa
    17341 atctggtgcg tcgcgacctg aatatggaag cggttgtcgc accagagatt tctgcgacgg
    17401 tgggcgtggc tgcggctttt gcggttaacc ccattgttgg agcggcagta tttgcagcca
    17461 gtaaagtgct ggggccgctg tggagcaaag tctccatttt gcgctatcac atttcgggtc
    17521 cgctggacga tccgcaaatc aacgaagtgt tgcgccaacc gcgtaaagaa aaagcgcaat
    17581 gatttgacga gggcgcgtaa ttgccccaat ctcatatgat aatcgttgcc aaaggccaac
    17641 gagccggaac ataactgtag gccggatagg gcgttcacgc cgcatccggc agccgtaaaa
    17701 aatcctctac tgcagtaact aacgagtagc aaaaacgatg agtctgaacc tggtaagtga
    17761 acaattgcta gcggcgaacg gcctgaaaca tcaggacttg ttcgcgatcc tcggtcaact
    17821 ggccgaacgt cgccttgatt atggcgatct ctattttcag tcgagctatc acgaatcctg
    17881 ggttttagaa gaccgcatta ttaaagatgg ttcttacaac atcgatcagg gcgttggtgt
    17941 gcgtgcaatc agtggtgaaa aaaccggatt tgcttacgct gaccaaatca gcctgctggc
    18001 gctggaacag agtgcgcaag cggcgcgcac catcgtccgt gatagtggtg atggcaaagt
    18061 acagacgctg ggcgcggtag agcatagccc gttgtatacc tcggtagatc cgctgcaaag
    18121 catgagccgt gaagagaagc tggatatcct gcgtcgcgtc gataaggttg cccgcgaagc
    18181 ggacaagcgc gtacaagaag tgactgccag cctcagcggc gtttatgaat taattctggt
    18241 tgcggccacc gacggcacgc tggcggcgga tgttcgtccg ctggtgcgtc tttccgtgag
    18301 cgttctggtc gaagaagatg gcaaacgcga acgcggtgcc agtggcggcg gcggtcgttt
    18361 tggttatgag ttcttccttg ccgatcttga cggtgaagtc cgtgcggatg catgggcaaa
    18421 agaagcagtg cgtatggcgc tggtcaatct ttctgccgtc gccgcaccag cgggcaccat
    18481 gccggtagta cttggcgcag gttggccggg cgtgctgttg catgaagcgg tggggcacgg
    18541 tctggaaggc gacttcaacc gccgtggcac ttcagtattt agtggacagg tcggggagct
    18601 ggtggcttca gaactgtgta ccgtggttga tgacggcacg atggtcgatc gccgtggttc
    18661 ggtggcgatt gatgatgaag gtacgccagg tcagtacaac gtgctgattg agaatggcat
    18721 cctgaaaggc tacatgcagg ataaactcaa cgcgcgtttg atggggatga caccgactgg
    18781 caacggtcgc cgtgaatcct acgctcatct gccgatgccg cgtatgacca acacctatat
    18841 gctgccgggt aaatcgaccc cgcaagaaat tattgaatct gttgagtacg gtatctatgc
    18901 accgaacttt ggtggcggtc aggtggatat tacatccggc aaattcgttt tctccacttc
    18961 agaagcgtat ctgattgaaa acggtaaagt aacgaagccg gtgaaaggcg cgacgttgat
    19021 tggttccggt atcgaaacca tgcagcagat ttcgatggtg ggcaacgacc tgaaactgga
    19081 taacggcgtg ggtgtctgcg gtaaagaagg gcaaagtttg ccggttggcg tgggccagcc
    19141 aacgctgaaa gttgataacc tgactgttgg cggtaccgcg taataatttc agtattttca
    19201 gaaggataat taatctttct acgtgcatga acggtcccct tgcccctctg tggagaaggt
    19261 taggggaacc cgttggcaca ggtttgtaca acgtaacagt acaatatgaa ttacttctct
    19321 ttcccgcgcc cgtgcatttc ctgaaacaat ttaccgacct caacaaaata atccgtcagc
    19381 gagttgatca cgacctgtac cttcagcggc agcttatctt tttcggtata taacgcataa
    19441 accgggcgtg gatctgactg ataacgcggc agcaggatct ccagctcacc gcgattgatc
    19501 tcgttgatca cccacatcag cggcacgtag gcgatcccgg cacccgccgt tagccagcgc
    19561 accagcgtca tcggatcatt agtcacaaat ctcccttgcg ggatcaggcg agtcgagatc
    19621 ccttccggtg caatcagttc aaattcattg tcgggccgca cgctgtattc aagccatgaa
    19681 tggctactta aatcggcggg tttttccggt atgccgtatt gtgtgagata gcttttcgcg
    19741 gcgcacacca ccattggcat cgcgccaaga cggcgggaaa acaggctgga atcctgcaac
    19801 gcgccgacgc ggatcaccac atccagaccg tcggcaatca ggtcgggggc tggaattccg
    19861 gtaaccagat tgacgctcaa acctgggtat tctttcagca ttttggctgt cagcccggcg
    19921 agaacatttt gtgccatagt tgaagaacag ccaatgcgta gcgtcccgat gggggtgtta
    19981 ttgaaggcat acagttgctc atgaacatcc tgcacttcat gaagcatacg acggcagccc
    20041 tggtagtaaa ttctaccggc ttcggtcagg ccaatgctgc gtgtgctacg gtttaacagc
    20101 tttacctgca actcatcttc cagttttgac accgtctgac tgatggacga aacgctcatc
    20161 tgtagctgtc tggcggcggc ggtaaaagag ccaaattcaa ctactttggc aaacaccgac
    20221 atgcgtttta gtcgttccat tattcactct gacttaaaag tgatttagat cacataatat
    20281 agataacagc ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct
    20341 cgcccggctt tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc
    20401 tctctataca accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc
    20461 cttcccaccg atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg
    20521 ggtacttgtg ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc
    20581 gctctattgc tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa
    20641 cactaataag aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca
    20701 tcgcaatttt taatgcctgg gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct
    20761 ttagcgctga cgtcgttgcg atcgcgccgg acgtttctgg actcattacc caggtgaatg
    20821 ttcatgataa ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct
    20881 atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg
    20941 agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag
    21001 agatcgacca ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg
    21061 cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg
    21121 gctgggtgac caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg
    21181 ttgcgctggt gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg
    21241 aaggggtgcg tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga
    21301 aagggactgt tgatagtgtt gccgctgggg tcaccaacgc cagcagcacg cgtgacgaca
    21361 aagggatggc gaccatcgac tctaaccttg aatgggtgcg tttggcgcaa cgtgttccgg
    21421 ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag
    21481 tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg
    21541 cccatcgcct gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca
    21601 tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca
    21661 cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg ctgccggtcc
    21721 ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgctatcgtg gctttttgcg
    21781 catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg
    21841 cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc
    21901 ctcgctggta cgaatagaaa actcgtatgc gtgggggctg gccggttata ccgcgctgat
    21961 cattgtgatc accattcagc ctgaaccatt gcttacgccg cagtttgccg tcgaacgttg
    22021 tagcgagatc gttatcggta ttgtgtgtgc gattatggcg gatttgctct tttctccgcg
    22081 atcgatcaaa caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt
    22141 aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct
    22201 ggtgcgacgc accacggcgc tacaaggtat gcgcagcaac ctgaatatgg aatcttcccg
    22261 ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac
    22321 ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt
    22381 ccgcgaattt tttgacacac cggtagaaac cgcgcaggac gtccacaagc agctcaaacg
    22441 cctgcgaaga gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg
    22501 ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat
    22561 caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtaaaagtag agtcagccga
    22621 acgtcatcat gcaatggtta acttctggcg aaccacactt tcctgcattc tgggaacgct
    22681 tttctggctg tggacgggct ggacttccgg tagtggtgca atggtgatga ttgcggtcgt
    22741 gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg
    22801 gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca
    22861 acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggattcttcc tcggtataga
    22921 agtacagaaa cggcgactgg gctcgatggg ggcactggcc agcaccataa atattatcgt
    22981 gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat
    23041 cgtcggctgt gtgctcgcgt tcaccgttat tttgctggtg cgggataaat cgcgcgacag
    23101 gactggacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa
    23161 tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat
    23221 gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgccca
    23281 ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca
    23341 aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta
    23401 ctttggtcag ttgctggaag aactggaaat ctaccaggaa aagctacgca tctggcaagc
    23461 tccaccgcag gtgacggaac cggttcatcg gctggcgggg atgctccata agtatcaaca
    23521 tgcgttgacc gatagttaag tcaaaaccga cgccaaaagc gtcggttttt tcatggctat
    23581 acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaacag atgaatattt
    23641 atacctttga ttttgatgag attgagagtc aggaggattt ttatcgtgac tttagccaaa
    23701 cctttggtct ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga
    23761 acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc
    23821 gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt
    23881 tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc
    23941 ggacaagacg atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct
    24001 acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat
    24061 ttcacgcata tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc
    24121 acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac
    24181 agaaagtacg cttaatgcag ctacagtggt tttgattttc atgattttta cctcgacata
    24241 atcttttagc tgggcctttg tttcgtgacc catatcacaa aatcaagtat acactaatta
    24301 ttgaactaa
//
LOCUS       DAMZII010000074        23697 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZII010000074 DAMZII010000000
VERSION     DAMZII010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.074"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(54..1502)
                     /gene="cusS"
                     /locus_tag="POA77_002821"
     CDS             complement(54..1502)
                     /gene="cusS"
                     /locus_tag="POA77_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDI6366221.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(1492..2175)
                     /gene="cusR"
                     /locus_tag="POA77_002822"
     CDS             complement(1492..2175)
                     /gene="cusR"
                     /locus_tag="POA77_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDI6366222.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2332..3711
                     /gene="cusC"
                     /locus_tag="POA77_002823"
     CDS             2332..3711
                     /gene="cusC"
                     /locus_tag="POA77_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDI6366223.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDAGRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQ"
     gene            3738..4070
                     /gene="cusF"
                     /locus_tag="POA77_002824"
     CDS             3738..4070
                     /gene="cusF"
                     /locus_tag="POA77_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDI6366224.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            4086..5309
                     /gene="cusB"
                     /locus_tag="POA77_002825"
     CDS             4086..5309
                     /gene="cusB"
                     /locus_tag="POA77_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDI6366225.1"
                     /translation="MKKNALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            5321..8464
                     /gene="cusA"
                     /locus_tag="POA77_002826"
     CDS             5321..8464
                     /gene="cusA"
                     /locus_tag="POA77_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDI6366226.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            8566..9942
                     /gene="pheP"
                     /locus_tag="POA77_002827"
     CDS             8566..9942
                     /gene="pheP"
                     /locus_tag="POA77_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDI6366227.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(10010..11257)
                     /gene="ybdG"
                     /locus_tag="POA77_002828"
     CDS             complement(10010..11257)
                     /gene="ybdG"
                     /locus_tag="POA77_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDI6366228.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(11365..12018)
                     /gene="nfsB"
                     /locus_tag="POA77_002829"
     CDS             complement(11365..12018)
                     /gene="nfsB"
                     /locus_tag="POA77_002829"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDI6366229.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEHIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(12112..12480)
                     /locus_tag="POA77_002830"
     CDS             complement(12112..12480)
                     /locus_tag="POA77_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI6366230.1"
                     /translation="MDKQSLHETAKRLALELHFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            complement(12545..12793)
                     /locus_tag="POA77_002831"
     CDS             complement(12545..12793)
                     /locus_tag="POA77_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDI6366231.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(12859..13977)
                     /locus_tag="POA77_002832"
     CDS             complement(12859..13977)
                     /locus_tag="POA77_002832"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI6366232.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            14419..14571
                     /gene="hokE"
                     /locus_tag="POA77_002833"
     CDS             14419..14571
                     /gene="hokE"
                     /locus_tag="POA77_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDI6366233.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            14922..15074
                     /gene="hokE"
                     /locus_tag="POA77_002834"
     CDS             14922..15074
                     /gene="hokE"
                     /locus_tag="POA77_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDI6366234.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(15196..15816)
                     /gene="entD"
                     /locus_tag="POA77_002835"
     CDS             complement(15196..15816)
                     /gene="entD"
                     /locus_tag="POA77_002835"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDI6366235.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(15991..18231)
                     /gene="fepA"
                     /locus_tag="POA77_002836"
     CDS             complement(15991..18231)
                     /gene="fepA"
                     /locus_tag="POA77_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDI6366236.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFHLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            18474..19676
                     /gene="fes"
                     /locus_tag="POA77_002837"
     CDS             18474..19676
                     /gene="fes"
                     /locus_tag="POA77_002837"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDI6366237.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            19679..19897
                     /gene="ybdZ"
                     /locus_tag="POA77_002838"
     CDS             19679..19897
                     /gene="ybdZ"
                     /locus_tag="POA77_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDI6366238.1"
                     /translation="MAFSNPFDDPLGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            19894..>23697
                     /gene="entF"
                     /locus_tag="POA77_002839"
     CDS             19894..>23697
                     /gene="entF"
                     /locus_tag="POA77_002839"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDI6366239.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQD"
BASE COUNT         5492 a         6050 c         6473 g         5682 t
ORIGIN      
        1 tgatcaacaa taccaggagg tgatgcacca cgccctgaat ttgaacttaa taatcactct
       61 ctctccggca gcataatcac aaaccttgtc ccccgcgcat ctgacgttac cgcaaccgtg
      121 cctttatgcg cgacaacaat cgatttcact atcgccagcc caataccgct accttcacct
      181 tttcgctggc gggaagggtc aacgcgatag aaacggtcaa acaatcgcgg taagtgctcg
      241 ggcgcaatgg gcgtaccggg gttttcgacg gaaacttgca cctgatgatt gaccgtctgg
      301 cagcgcacta caatagcctc actgggtggc gtataacgca gggcattaga aagcaggttg
      361 cttaacgccc gacgcagcat cagcggatcg cccgcaacct gacatttgtc gccaacaaac
      421 cgcaactcca cgccgcgatc ttccgctaac gcctcgaaaa aatcgaacac tttgccgact
      481 tcatccgcca ggttgagcat tttcttttcg gggattagct ggttgttatc ggcctgagcg
      541 agaaacagca tatcgctgac cattttcgcc attcgcgtca gctcttcgag attagagtag
      601 agcacatctt ccagttcctt ctggctgcgc gactggctga gggcgatttc cgtttgcgtt
      661 atgagattag taattggtgt gcgaatttcg tgagcgatat ccgctgagaa attggactgg
      721 cgggtaaaga catcctcaat acgctcgatc atatggttga acgacagtac cagctgttcc
      781 agctcaatgg gcacggtctg cgggtcgagg cgaacgtcga gatctttcga ggtaatattc
      841 tggatttgac ggctgacgct gcggatcggc gcgtgacctt tatgtaccgc caatagcacg
      901 ataaagacga tcaggatgct gatgacagat gcggtcataa taagtttatt catcaaatca
      961 tttatgtaat gaagatgaaa atcgatcgaa agcgcgatgt agagcgtata aatcggtttg
     1021 ccgtccacca acgggccaac cggcaagtta atcatccgcc agttgctgtg ttccatatgc
     1081 ccgtgaccgt ggcctggcat catcatcgtc gggccggaaa ggagatacac ctcaccgccc
     1141 cgcgcgtctt tatcgggtat ggcgtcacgc gtaaactcgc ggatatccgg cgcaccgggg
     1201 gagtgataca ccgttttacc atggctatct gccagggaga tcaacacgtt ggaataacca
     1261 ctgacgatat cttccagcgt cattaagcgt cgggcttgcg tctcgtcagg atgattaagt
     1321 acccgttcaa gcgtggcgct aatttctttt aaatcattaa tatcctgctc ggcaaaatgc
     1381 acttttactg agtggatcat gatccatgca aaggcgaaaa acgctgcgat ggtggccagg
     1441 ctgataaaaa aggtcaggcg ggttgccagc gaaaacgggc gctgaaatgg cttactgacc
     1501 atccggcacc tcaagcatgt aacccacgcc gcgcacggtc tgaattagct ttggctcaaa
     1561 gtcgttgtcg attttgccgc gcagccgctt caccgctaca tcaatggcgt tagtgtcgct
     1621 gtcgaaattc atgtcccaca cctgcgaggc gataagcgag cggggcagca cttcgccctg
     1681 atggcggagg aaaaactcca gcagagtaaa ctctttactg gtcagagtga tgcgcgtgcc
     1741 gctgcgggtg actttgcggc tgacgagatc gaccatcaga tcggcaacct gaaactgact
     1801 ttcgataatc accgccgccc cgcgccgcag gagagtgcgc acccgcgcca gcagttcagc
     1861 aaaagcgaac ggcttcacca gatagtcatc cgcccccaac tccagcccct tgacgcgatg
     1921 ttcaatggtg ccaagcgcgg taagcaacag aatcggcatc cctttattgg cggaacgcaa
     1981 catgcgcacg atatcccagc cgttcacgtc cggcagcata atatcgagga ttatcagatc
     2041 ataatcaccg gtcatcgcca gatggtagcc attcaacccg ttgtcggcca aatcgaccac
     2101 aaaaccggct tcggttaacc ctttggtcaa gtattctccg gttttctttt catcttcgac
     2161 aatcaacagt ttcatatttc ctccgcatgt tgcccgggca attctagagt agcgggatca
     2221 gatggcaatc gcttattggc aaaatgacaa ttttgtcatt tttctgtcac cggaaaatca
     2281 gagcctggcg agtaaagttg gcggcataaa atcaccagaa attatgagcc tatgtctcct
     2341 tgtaaacttc tgccattttg tgtggccctt gcgctaaccg gttgttcact ggcaccggat
     2401 tatcagcgtc cggcaatgcc cgtgccgcag cagttctcac tcagccagaa cggcctggtt
     2461 aacgcagcag ataactatca gaacgcgggc tggcgcacct tttttgttga taatcaggtg
     2521 aagacgctga ttagcgaggc gctggtgaat aaccgggatt tgcgcatggc gacgctgaaa
     2581 gtgcaggagg cacgggcgca atatcgtctg accgatgccg gccgctaccc acagctcaat
     2641 ggcgagggca gcggcagctg gagcggtaat cttaaaggga ataccgccac gacacgggag
     2701 ttttcgaccg gccttaacgc cagctttgac ctcgattttt tcggtcgctt aaagaacatg
     2761 agcgaagccg agcgacaaaa ttatttagcc actgaggaag ctcagcgcgc ggtgcatatt
     2821 ctgctggttt ctaatgtcgc gcaaagctat ttcaatcagc aactggcgta tgcgcaattg
     2881 caaatagccg aagaaacgct gcgtaattat cagcagtcat atgcgtttgt cgaaaaacaa
     2941 ctgttgaccg gtagcagcaa tgttctggcg ctggaacagg ctcgcggggt gatagaaagt
     3001 acccgcagtg acatcgctaa acgtcagggg gaactggcgc aggcgaataa tgcattgcaa
     3061 ctgttattgg gaagctacgg caagctgccg caagcgcaga cagtaaacag cgacagcctg
     3121 caaagcgtta aattaccggc gggcttgtcg tcgcaaatct tattgcagcg cccggatatt
     3181 atggaagctg aacacgcgtt aatggcggct aatgctaata ttggtgctgc gcgtgcggca
     3241 tttttcccct ctatcagcct gaccagtgga atatcaaccg ccagtagcga tctatcctca
     3301 ttatttaacg ccagcagcgg gatgtggaat tttataccca aaattgagat ccccattttt
     3361 aatgccggac gcaaccaggc caatctggat atcgccgaaa ttcgccagca gcagtcggtg
     3421 gtgaattatg aacagaaaat ccagaacgcc tttaaagaag tggcagatgc gctggcatta
     3481 cgtcaaagcc tgaacgatca aattagcgcc cagcagcgtt atctggcgtc gctgcaaatt
     3541 accctgcaac gggcacgggc attatatcag cacggcgcgg taagttatct ggaagtgctg
     3601 gatgccgagc gctctttatt tgcaacccga caaactttac ttgacctgaa ttatgcccgt
     3661 caggttaacg aaatttcttt atataccgca cttggtggcg gttggcagta gtaattttta
     3721 actccaggag agaataaatg aaaaaagcac tgcaagtcgc aatgttcagt ctgtttaccg
     3781 ttattggctt taatgcccag gctaacgaac atcatcatga aaccatgagc gaagtacaac
     3841 cgcaggttat tagcgccact ggcgtggtaa agggtattga tctggaaagc aaaaaaatca
     3901 ccatccatca cgatccgatt gctgccgtga actggccgga gatgaccatg cgctttacca
     3961 tcactccgca gacgaaaatg agtgaaatta aaactggcga caaagtggcg tttaattttg
     4021 tccagcaggg caacctttct ttattacagg atattaaagt cagccagtaa cccaggttta
     4081 atgagatgaa aaaaaacgcg cttattatcg gcagcatgat cgcgggcggt attatttctg
     4141 cggcaggttt tacctggttt gcaaaggctg aaccacccgc agaaaaaacg tcgaccgcag
     4201 aacgtaaaat cttattctgg tacgacccga tgtaccccaa tacgcggttc gataaaccag
     4261 gtaaatcgcc gtttatggat atggatctgg tgccgaaata tgccgatgaa gagagttctg
     4321 cgtctggtgt gcgcattgac ccgactcaga cgcagaatct gggggtgaaa acggctaccg
     4381 tcacgcgcgg gccgctgact tttgcccaga gtttcccggc gaatgtcagt tacaacgagt
     4441 atcagtatgc cattgtgcag gctcgcgctg ccgggtttat cgacaaggtg tatccgctta
     4501 ccgtgggcga taaagtacaa aagggcacac cgcttctcga cctgaccatt cccgactggg
     4561 tggaagcgca gagtgagtat ttactgctgc gcgaaacggg cggtacggcg acccagactg
     4621 aaggcattct tgagcggctg cgactggcgg gaatgccgga ggcggatatt cgccgcctga
     4681 tcgccacgca aaaaatccag actcgcttta cgctcaaagc acccattgat ggtgtgatca
     4741 ccgcgtttga tctgcgcgcg ggaatgaata tcgccaaaga taacgtggta gcgaaaattc
     4801 agggtatgga cccggtgtgg gtcactgctg cgatcccgga gtctatcgcc tggctggtga
     4861 aagatgcctc gcagtttacg ctcaccgttc cggcacgacc ggataaaaca ctcaccatcc
     4921 gcaaatggac gctgctacct ggcgtggatg ccgcgacccg cacgctgcag ctgcgtctgg
     4981 aagtcgacaa cgccgacgag gcgctaaaac cgggcatgaa cgcctggttg caactcaaca
     5041 ccgccagcga accgatgctg ctcattccgt cacaagcgct gattgatacc ggcagcgaac
     5101 agcgggtgat taccgttgat gccgacgggc gctttgtacc gaaacgcgtt gctgttttcc
     5161 aggcatcgca aggcgtcacc gcattacgct ctggtctggc ggaaggtgaa aaggtggttt
     5221 ccagcggcct gttcctgatt gattccgaag ccaatatttc tggcgcactg gagcggatgc
     5281 gctctgaaag tgctacccat gcgcattgag ggaataacca atgattgaat ggattattcg
     5341 tcgctcggtg gcgaaccgtt ttttggtgct gatgggcgcg ttgtttctga gcatctgggg
     5401 cacctggacc atcattaaca cgccagtgga tgcgctgccg gatctctccg atgtgcaggt
     5461 gattattaaa accagctatc ccggtcaggc accgcaaatc gttgaaaatc aggtgactta
     5521 tccgctaacc accaccatgt tgtcggtgcc tggcgcgaag actgtgcgcg gtttctcgca
     5581 gtttggagac tcttatgtgt atgtcatttt cgaagatggt accgatccgt actgggcgcg
     5641 ttcgcgggtg ctggagtacc tcaaccaggt acagggtaag ctgcctgcgg gagtcagcgc
     5701 cgagctggga ccagatgcca cgggtgttgg ctggatctat gaatatgcac tggtggatcg
     5761 cagcggtaag cacgatctgg ccgatttacg ctcattgcag gactggtttc tcaaatatga
     5821 gctaaaaacc atccctgacg ttgcggaagt ggcgtcggtg ggcggcgtgg tgaaagagta
     5881 tcaggtggtt atcgatcccc agcgcctggc gcaatatggc atcagtctag ccgaagtaaa
     5941 aagcgcgctg gatgcttcaa accaggaagc gggcggttcg tcgatcgaac tggcggaagc
     6001 ggaatatatg gtgcgcgcca gcggctatct gcaaacgctc gacgacttta atcacatcgt
     6061 tttaaaagcc agtgaaaatg gcgtgcccgt ttatctgcgc gatgttgcga aggtccagat
     6121 tggcccagag atgcgccggg gcattgccga actaaacggc gaaggcgaag tggcgggcgg
     6181 ggtggtgatc ctgcgatccg gcaaaaacgc ccgagaagtg atcgccgccg tgaaggacaa
     6241 actggaaacg ctgaaaagta gtctgccgga aggcgtggag atagttacaa catacgatcg
     6301 cagccagctc attgaccgcg ccatcgacaa cctcagcggc aagttgctgg aagagtttat
     6361 tgtggtggcg gtagtctgtg cgctgtttct ctggcatgtg cgctcggcgc tggtggcgat
     6421 tatttcgttg ccgctggggt tgtgtattgc ttttattgtc atgcacttcc agggactgaa
     6481 tgccaatatt atgtcgttgg gcggcattgc gattgccgtc ggggcgatgg tcgatgccgc
     6541 tatcgtcatg atcgaaaatg cacataaacg gctggaagag tggcagcacc agcatcctga
     6601 cgccacgctg gataataaaa cgcgctggca ggtgatcacc gatgcatctg ttgaagtggg
     6661 tccggcgcta tttatcagtc tgctgattat cacgttgtcg tttatcccga tcttcaccct
     6721 ggaagggcag gaagggcgtc tgtttggccc gttggcgttc actaaaacct atgcgatggc
     6781 gggcgcggcg ctgctggcga tcgtagtgat ccctatcctg atggggtact ggatccgtgg
     6841 caaaattccg ccggaaagca gtaacccgct caatcgcttt ttgattcgtg tttatcatcc
     6901 gctgttgctg aaagtactgc actggccgaa aaccacgctg ctggtggcag cgctttcggt
     6961 gctgacggtt ctctggccgc tcaataaagt tggcggggaa tttttaccgc agatcaatga
     7021 aggcgacttg ttgtatatgc catcgacgct gccggggatt tccgcagcag aggcggcgag
     7081 tatgctgcaa aaaaccgaca agctaattat gagcgtacct gaagtggcgc gggtatttgg
     7141 caaaaccggg aaagcggaaa ccgccaccga ttctgctccg ctggagatgg tagaaacgac
     7201 catccagctt aagccgcagg agcagtggcg gccaggcatg acgatggaca aaatcattga
     7261 ggaactggat aacaccgtgc gtctgccggg gctggcgaat ctgtgggtgc cgccaattcg
     7321 taaccgtatc gatatgctct caaccggcat taaaagcccc atcggcatta aagtttccgg
     7381 cactgtgctg gcggatatcg acgcgatggc tgagcaaatt gaagaagtgg cgcgaacggt
     7441 gccaggcgta gcttctgctc ttgccgagcg gctggaaggt gggcgctata tcaacgttga
     7501 gattaaccgt gaaaaagccg cgcgttacgg tatgacggtg gcggatgtgc agttgtttgt
     7561 gacttctgct gtgggcgggg cgatggttgg cgaaacggtg gaaggaattg cccgttatcc
     7621 aattaatctg cgttatccgc aaagctggcg cgatagtccg caggcactgc gccagctgcc
     7681 gattctgacg ccgatgaagc agcaaatcac cctggcagac gtggccgaca ttaaagtctc
     7741 taccggaccg tcaatgctga aaaccgagaa tgcgcgcccg acgagctgga tttatatcga
     7801 tgcccgcgat cgtgacatgg tgtcggtggt tcacgatttg caaaaagcga tagctgaaaa
     7861 agtgcagtta aaaccgggca ccagcgtggc attctccggg cagttcgagc tactggagcg
     7921 cgccaaccat aagcttaaac tcatggtgcc gatgacgttg atgattatct tcgtgctgtt
     7981 gtatctggcg ttccgtcggg tgggcgaagc gttgctgatt atcagcagcg taccgtttgc
     8041 gctggtgggc ggcatctggt tgctgtggtg gatgggcttt catctttccg tggcgacggg
     8101 cactggcttt atcgccctcg ccggggtcgc cgccgaattt ggcgtggtga tgctgatgta
     8161 tttacgtcac gccatagagg ccgagccgtc gttgaataat ccgcaaacat tcagtgagca
     8221 gaagctggat gaggcgttat atcacggcgc ggtcctgcgc gtgcgcccga aagcgatgac
     8281 ggtggcggtg attatcgccg gtctgctgcc gattctgtgg ggaacggggg ctggttcaga
     8341 ggtgatgagc cggattgccg caccgatgat tggcggcatg atcaccgcac ctttgctgtc
     8401 gctgtttatt atcccggcgg cgtataagct gatgtggctg caccgacatc gggtacggaa
     8461 ataaaagcag gataccccgt ttaaccgtgt ggattgtgtc ttgcgacgat gggcactaaa
     8521 tgttaaaagg tgcccctcaa caaaaaagac acacagggga aaggcgtgaa aaacgcgtca
     8581 accgtatcgg aagatactgc gtcgaatcaa gagccgacgc ttcatcgcgg attacataac
     8641 cgtcatattc aactgattgc gctgggtggc gcaattggta ctggtctgtt tcttggcatt
     8701 ggcccggcga ttcagatggc gggtccggct gtattgctgg gctacggcgt cgccgggatc
     8761 atcgctttcc tgattatgcg ccagcttggc gagatggtgg tcgaagagcc ggtatccggt
     8821 tcatttgccc actttgccta taaatactgg ggaccgtttg cgggcttcct ctctggctgg
     8881 aactactggg taatgttcgt gctggtggga atggcagagc tgaccgctgc gggcatctat
     8941 atgcagtact ggttcccgga tgttccaacg tggatttggg ctgccgcctt ctttattatc
     9001 atcaacgccg ttaacctggt gaacgtgcgc ttatatggtg aaaccgagtt ctggtttgcg
     9061 ctgattaaag tgctggcgat catcggtatg atcggctttg gcctgtggct gctgttttct
     9121 ggtcacggcg gcgagaaagc cagtatcgac aacctctggc gctacggtgg tttctttgcc
     9181 accggctgga atgggctgat tttgtcgctg gcggtaatta tgttctcctt cggcggtctg
     9241 gagctgattg ggattactgc cgctgaagcg cgcgatccgg aaaaaagcat tccaaaagcg
     9301 gtaaatcagg tggtgtatcg catcctgctg ttttacatcg gttcactggt ggttttactg
     9361 gcgctctatc cgtgggtgga agtgaaatcc aacagtagcc cgtttgtgat gattttccat
     9421 aatctcgaca gcaacgtggt agcttctgcg ctgaacttcg tcattctggt agcatcgctg
     9481 tcagtgtata acagcggggt ttactctaac agccgcatgc tgtttggcct ttctgtgcag
     9541 ggtaatgcgc cgaagttttt gactcgcgtc agccgtcgcg gtgtgccgat taactcgctg
     9601 atgctttccg gagcgatcac ttcgctggtg gtattaatca actatctgct gccgcaaaaa
     9661 gcgtttggtc tgctgatggc gctggtggta gcaacgctgc tgttgaactg gattatgatc
     9721 tgtctggcgc atctgcgttt tcgtgcagcg atgcgacgtc aggggcgtga aacacagttt
     9781 aaggcgctgc tctatccgtt cggcaactat ctttgcattg ccttcctcgg catgattttg
     9841 ctgctgatgt gcacgatgga tgatatgcgc ttgtcagcga tcctgctgcc ggtgtggatt
     9901 gtattcctgt ttgtggcatt taaaacgctg cgtcggaaat aaggcattca cgctacatcc
     9961 gacaaaacga tgtccgctct catccattcg atgagagcgg tttttttaat tactgcttaa
    10021 atgcacccgc cagagagcga atatcattgc cggttggcga ctgatgaagt cgcagaccaa
    10081 actcttcgac aatcgcaaat atgtgatcga atatatcagc ctgaatgctt tcatattcca
    10141 gccacaccac ggtgttggta aacgcataga tctcgagcgg taaaccgtta tcacctggag
    10201 ccagttggcg taccattaaa gtcatatctt tacgaatccg cggatggtta cgcagatatt
    10261 cgttcagata ggcacgaaag gttccaatat tggtcattcg gcgcaggttt aatatcgact
    10321 ccgtagaacc ttgctggcga ttccactcat taatttcctg atggcggctg gttaaataag
    10381 gctttaacaa atgcgcttta ttcagacgtt gcatttcgtc ttcatcaaga aaacgaatac
    10441 tggtgacatc aatactgata ctgcgcttaa tacgtcgccc gccagatgct gacatcccgc
    10501 tccagttttt aaaggagtca gaaaccagag accaggtggg aatagtggta atggtattgt
    10561 cccagttacg cactttgacg gtggttaacc caatatcgat caccgcgcca tccgcgccgt
    10621 atttcggcat ctccagccag tcgcccagtt tcagcatatc gttcgcggaa agctgaatac
    10681 ctgccaccag accaagaatc ggatctttaa aaaccaacat cagcacggca gccattgcac
    10741 caagaccgct gatcagaatc gctggcgact gaccaatcag cagcgagatc atcaaaatgc
    10801 cgaccagaat cgcgccgatc agtttaatcc cctgaaatat ccctttcagc ggtaactgag
    10861 atgccgccgg gaatttctgc gccagattca aaatcacatc cagcaacgag aagactgaaa
    10921 gcagcgcata catcatgatc cacaactgcg cgcaggtagt cagaatatct gccgcttcgg
    10981 tgcctttttg cagccagaat accgcctgaa tattgacgat aatcccctgc agggtaaaag
    11041 ctaaacggtg gaagagttta ttctgggtaa tgatttgcaa ccaaagccgt gaactggcga
    11101 tggcacgttt ttcgaaggtc cgcagtacca cccaatgcaa aataatatgc accacgacgg
    11161 cggtcagaaa aataataccg aaaatcatca ccatcgaggt ggtgtgatcg atctcaatac
    11221 ccgctaaatc ttcaacctgg gatattaaat cctgcataac gtctccttta tacaacagca
    11281 gcctatgatg acggctgaaa cagggttatg caaatcagga gaatctgagg ggaaatagcc
    11341 gggcagatgc ccggcaagag agaattacac ttcggttaag gtgatgtttt gcggcagacg
    11401 tgatttcggc agcgtagcgt taaaatcttc aacgctgtga tgacctaccg gaacaaccac
    11461 cagactggtg tagcctttct ctttcagacc aaattctgca tcgaggatgg cggcgtcaaa
    11521 accttcgatg ggtaccgcgt ccagacccag cgccgccacg ccgagcagga agttaccgac
    11581 gttgagataa acctgttttg ccatccactc tgcatcatca tgcagatctt tacggtgcat
    11641 atcagcgaag aacttgcgac ctttatcgtt cgcggctttc gcttccggcg tggcaaagcg
    11701 gccatcagca tcttcctggt caacaaccag cttcagccag gcatcgtcca tcgcggtttt
    11761 tgcacagaac accacgacgt gcgaggcatc aagtattttg cgttcgttga acacataatt
    11821 accggcagcg gatttggcaa cacgcgcttt accttcttcc gtgctggcaa caataaaatg
    11881 ccacggctgg gagttggtgc tggatgggct gtattgcagg agagttttga tatgctcggc
    11941 ctgttccggg gtaagttttt tactggcatc aaatgcctta gtggaatgac gctttaaggc
    12001 gacagaaatg atatccataa agactccatg tgaaagtaat tttgcgtgcc agcagattac
    12061 aaggttcaac gagaaatggt aagcgagaaa aatgcgctat agatttccgc tttagcctgg
    12121 acgcactctt ttttgatcgc gtttagccag accatcaacc accagattcc acgaatcgtt
    12181 gatcagatcg cgaagtaacg cttcggagat ttcctcgccg ggatacaccg aaatccagtg
    12241 ctttttattc atgtgatacc ctggcttaat gctcgggtat atctgctgat ttaacaggga
    12301 tttttgtgga tcggacttca gattgataaa ggggacgccg cgcagctccg acgacagcat
    12361 aaaaattttg ccgccaattt taaaaacatc gaactccggg ccaaaaggcc agcaaagctc
    12421 gacaaagtgt aactcaaggg ccaggcgttt cgccgtttcg tgcagtgatt gcttatccat
    12481 aaacgttcct ttaggcgaag gagaataagc aaagtatgcc gcgaagtacg gcgataatcg
    12541 acgtttaatc cgccagcgag aaccagcgtc gccagataaa gcgcagaaca aaatactcaa
    12601 tagcgcccag cactaaaaac cacagacaaa acaataaagt gtaaagctgg ctaagatcca
    12661 tcagatggaa catggtcacc agtttttgtg ccagcgtcag ccccagtgcg ggggcgggca
    12721 gcagcaggca agagaggaaa gccatcagca aaatgcctgc tgcggtagtc aaggtttcta
    12781 aagggtgttt cataacattg ttaaatgtaa gttaaaacac cattgtcagg gatattcttc
    12841 tgtaaggcaa ttccctactt agtcaccggc ccagatctca caatgctttt tcaccagccc
    12901 aatcagcgag ccaccatcgg cgacgaaatc gcgcatcagc tgcgcttcat tcagaccgct
    12961 gacgacctgg cgatgcaggg cctcaatcgc gctcgatgca cccattttat gtgcggaagg
    13021 ggcgattttt tccagcaatc gcaaggtatc ttccgttagc ggtcgacgat cgccagtgtg
    13081 cggatcggta atgacgcctt ccagcccata gcggcaggcc tggaaacggt tgaatttata
    13141 cagcaggtaa tctttctcct gatgtttaaa cgggcgttcc gtcagtaacc agtgggcggt
    13201 ggcctgaatt aatcccgcca tatttaccgc gtggctaagg gttaacgggg tatccatcac
    13261 ccgaacctcc accgtgccaa aatgaggact ggggcgaata tcccagtgca gatctttaat
    13321 gctgtcgatc atcgtggtgt aactcaggca gcgaaacagg gcttcaaatt gttgccagtt
    13381 actgacccac ggcatcgggc cattatcggg aaaggcggaa aaaatattcg gtcgtgagga
    13441 ggcaaaacgc gtatccgttc cctgcatata cggcgacgcg gcggaaaggg cgatgaagtg
    13501 cggcacaaac cgtgacaggc cgtgcagcaa ataaatggcg tcatcgccac tggcgcagcc
    13561 aacatggaca tgctgaccaa aaacggtcgc ctgctggatg agatagccaa agttttccag
    13621 cgttcgttga tagcgttcgt tgtcgcatac ctcctgacgc tgccatttct gaaacgggtg
    13681 cgtgccaccg ccgcaaattt ccagatgatg gtctgcggct gcctgcaata cgactttctg
    13741 catcgctgaa aactgcccgg cagcctggtt gatatcacgg caaacatccg tcgccagctc
    13801 cagcatactt tcggtgatat cgtgctttac ctctccggcc gtgatctcat ttttaaccgc
    13861 gtcaatcagc attgaagagt cctggcttaa gtcatagccc ggcggattaa ccacctgcat
    13921 ttccagttca ataccgaggg taaaaggttc agaaacatga aaatcgggta atggcatagg
    13981 tttctcttaa gttggcgttt tctattcagt atagaagtcg gagcggctgg gcgagatgcg
    14041 gaagttctgg aatgtttctt tttttggtga tggtgactga agcaatttgg ctacttttgc
    14101 aatgtgacaa gttatggcac ggctggctgg tggcgaagaa ttttgacgat tgaggcatgc
    14161 agaaaaaaac gggttcagct tcagttgatc aaatggggag tgagaagtcc gaaacaggac
    14221 ttactgtata aataaacagc tattttgcta agcaagggta agataactgc gggtgcctga
    14281 agctttccgg tttcaggttt actctgaggt ctggaaagat gaagccccag gacgatattt
    14341 ctatcaaccc tggggctacc tacccaaacc tgaacaattt ggatggtagt cccttcttcg
    14401 catggaggca atataaacat gctgacgaaa tatgcccttg cggcagtcat agtgctgtgt
    14461 ttaacggtgc tgggatttac gcttctggtc ggggattcgc tgtgtgagtt caccgtaaag
    14521 gaacgtaata ttgagtttaa ggttgttctc gcttacgaac cgaagaagta gccgttgtgc
    14581 ggggagcaat ccccgcatat ccggttgtca ggtcaggatg gtaaggcacc tgctttacac
    14641 tttcgcccgc ggtcagtgat ggctgcgggc gatttcgttt taagagcaat gatgagtttt
    14701 gccgacagaa gtccgaaaca ggattcactg tataaataaa cagctatttt gttgaggaag
    14761 ggtaagataa cggcgggtgc ctgaagcttt ccggtttcag gttaactctg aggtctggaa
    14821 agatgaagcc ccaggagata tttctatcaa tcctggggct gccactccga gcctcaacaa
    14881 ctcggatggt agtcccttct tcgcatggag gcaatataaa catgctgacg aaatatgccc
    14941 ttgtggcagt catagtgctg tgtttgacgg tactgggatt tacgcttctg gtcggagact
    15001 cgctgtgtga gttcaccgta aaggaacgta atattgagtt taaggctgtt ctcgcttacg
    15061 aaccgaagaa gtagccgttg tgcggggagc aatccccgca tatccggttg tcaggtcaga
    15121 gtggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgaatcgt
    15181 accagatgtt gtcaattaat cgtgttggca cagagttatg actatttttt cttttatctg
    15241 ccagtgcaca gcaaacatct cattctcacg atgaatgatg acctgctgtt tattccagct
    15301 aattatctga tagtccagaa aacctgcatc agtttggatc tcacttgcct taaatgcgct
    15361 ctctttggcg gaaaatgcca gtgtcagcgc cagagaaaag gctaaaccgc agtctgcgag
    15421 tcgctcgtgt tctgctggtg taataatgtt gtctgtcaat tcggtcgctg tttgcgcaga
    15481 aaaaatttct tcgatatcaa cgccaatcgg ttgacgggaa accacggcca atgccgtagc
    15541 cccgcagtgg ctaatactgc cgtatacctc cgcaggccac accggttggc gtagctcgcc
    15601 gattgcgggc acacatttat agccatattc ccgcagcgca taaattgcag cgatccgccc
    15661 ggctaaatgt tcggctttgc gcttccgtcc agcgtgttgc agttgcgcgt aatgcggcag
    15721 ccagagtaaa tcctgctcac aaaaattcgc cggatcgaac tcaacaaaat gcagcgtatg
    15781 tccggcaaag gggagggagg tatgcgtagt tttcatgtcg accatattag agactgatga
    15841 caaacgctaa actgcctgat gcgctacgct tatcaggcct acatggtcct gcaatatatt
    15901 gaattggcaa gatttttgta ggccggataa ggcgttcacg ccgcatccgg catgaacgac
    15961 gcgcactttg tcaacaatct gacgttagca tcagaagtga gtgtttacgc tcatgtacca
    16021 cgtacgtccc ggctcgttat aggtatacgc cccggcaccc gcgatatagt tggcccctgc
    16081 cagatcgccc gtggtctggg cattacccgc acgccacaaa cgtttgtcga acaggttgtc
    16141 cacgccgccg gtcagactga cattcttcgt cacatcccag gtcgcgctca gacccacaat
    16201 gctgtaagga ctgatttctt tggtttccgg gccaaccgct ggctgacctt tgtagttgta
    16261 ttttttcggc tgctgcttac cgtaccaggt gaaggtcgtt tgcatcgaca aatcttcccg
    16321 cgcctgccag ctcagcgttg agttcaacgt gtactccggg atgatcgaca aacggtcgcc
    16381 cgtggttttg ttttcactct tcagcatata agtgatgtta ttggtccaca tcaccgtttc
    16441 gctaactggt acgtttaacg atccttccag accttcaacc actgctttcg gcacgttatc
    16501 ccactgatag agatcggtgc cgactgcgtt ttgccctaca gccacatagc ccgcttcaat
    16561 cttattgcga taatcgttac ggaaccaggt gacgcccgcc agccagccgt cgcgtttgaa
    16621 ctccagacca atctctttgt tgatgctggt ttctgctttc aggtcatcgt taccttgcag
    16681 atagcagccg cccgcgctgg catagcagcc ctgaccttta ctgtagagaa tgtagttcgg
    16741 gttagtctgg tacaggctcg gtgctttata agcacgggca atgcccattt tgagcgtgaa
    16801 gtcatcgcct aaaccttgcg atatgttcag cgccgggctc cagttattgc cgacaatact
    16861 gtgatgatcg aaacgcagcc ccggcgttac gatggtgctg tcagtcagct ccatgttgtt
    16921 ttcggcaaac agcgagaaaa tttctgcttt tgaatacggg ctacggtcgg tggcactcac
    16981 accatcaata gcgccgccgg tgttcgcccc ggtcagcgcc tgggtattgg aactcaagtc
    17041 cttcatccgt tgctgattcc actccgtacc cagcgtcagc gtctggttaa cgaggaaatc
    17101 aatcggcagg ttaacttcgc tgtgcagcat cacgtcatcg agatcgatat cgacgaaatc
    17161 ctgtgtcgct ttttcgttaa atttcccttc ggtaccgccc gccagacctt ccggaatacg
    17221 cgagttacgg gtgtgttcat actgcaccca gttgctggtg gtcacgccgt tatcccagcc
    17281 accgttccag gtcagcgcgt agttctggcg atacaggcgg ttggtttcat cgccatattt
    17341 cgagcgagtg taagcgtcag agttggtgtt ctgggtatcg cccgcataca ggttaccctg
    17401 gcggctgtaa cccgcttcca gttccagcga ttgcaatggt gcgaagtccc agcgcaccac
    17461 gccgttaata tctttattga tcaccccttc gcgcccggct ggtaacgtcg tggcatacgt
    17521 tccggcacgc gcggactgat ggccctggtt gatatcccac gcgtcagcct gggttttgtc
    17581 gaggttgcca tacaagtgga agctgaattc gtcgcccagc ggaccggtca ggctgaagtt
    17641 ggtgcgttta gtggcacctt cctctttatg ttctggtgca ttgaaataag cgtcccagga
    17701 gccgtgccac tcaccgctgc cttttttggt aatgatgtta accacgccgc ccgccgcgcc
    17761 gttgccataa cgcgcagctg ccggaccacg cagaacttca atacgttcaa tcatttcagg
    17821 tggcacccag gaggtatcac cacgggtatc gcgctcgcca cgccagccct gacgcaccga
    17881 gttacggctg cttaccggct tgccgtcaat caaaatcagc gtgttttccg gacccatacc
    17941 gcgaatatca atctgtcggt tattcccacg ctgaccactg gtggagttac cggtcaggtt
    18001 aacgcctggc atggtacgga tgatctccga cacatcgcgg gcaacggggt ttttgcggat
    18061 ttcatctgcg gtgatggtcg aaacgccagg cgcctgtaag ttctgctcgg cggcggtaac
    18121 gacaatagta tcgtcatgtg aaacaggagt atcggtcggc tcttgtgcct gtgctacccc
    18181 ataaatcccc agattgacca acaaggccag ggaatgaatc ttcttgttca ttgttttatt
    18241 cctgcatttt tgccacgaat tgcaactgtc gggcatggtc gtcctcaaca cgacgcatcc
    18301 cgctaccgcg aaaacctttg atcctgaaag acacgcagtg cagttggtta ataaatgtcc
    18361 gcgcttccca cggcgcgcca ttacgctatt gcaaatgcaa atagttatca ataatattat
    18421 caatatattt ctgcaatcaa tgaaaaattg cacagtaaac atggggttat ggtgtgacgg
    18481 cgttaaaagt aggaagtgag agctggtggc agtcgaaaca tggcccggaa tggcagcgtc
    18541 tgaatgacga aatgtttgag gtcactttct ggtggcgtga tccccaaggt tctgaagaat
    18601 actcgacgat aaagcgcgta tggatctaca tcactggtgt gaccgatcat catcagaaca
    18661 gccagcccca gtcgatgcag cgaattgcag gtactgatgt ctggcagtgg acgacacaac
    18721 tcaatgccaa ctggcgcggc agctactgct ttattcccac cgaacgcgat gacatttttt
    18781 ctgcaccatc ccccgatcgc ctcgaattac gtgaaggctg gcgaaaacta ttaccccagg
    18841 caatagccga tccgctgaat ccacaaagct ggaaaggcgg gcgagggcac gctgtttctg
    18901 cactcgaaat gccgcaagcg cctctgcaac cgggatggga ttgtccgcaa gcgccagaaa
    18961 tacctgccaa agaaattatc tggaaaagtg aacggttgaa aaattcacgg cgtgtatgga
    19021 tttttaccac cggcgatgca acagcagaag aacgcccgct ggcagttttg ctcgatggcg
    19081 aattttgggc gcaaagtatg cccgtctggc cagtgctgac ttcgctgacc catcgtcagc
    19141 aacttcctcc cgccgtgtat gtgttgatcg acgctatcga caccacgcac cgcgcccacg
    19201 aactgccgtg taatgcggat ttctggctgg cagtacagca agagttatta cccctggtga
    19261 aagctattgc cccttttagc gatcgcgccg atcgcaccgt ggtcgccggg cagagttttg
    19321 gtgggctgtc cgcgctgtat gccggactgc actggcctga acgctttggc tgtgtattaa
    19381 gccagtcagg atcgtactgg tggccgcatc ggggcgggca gcaagagggc gtgttacttg
    19441 aaaagcttaa agctggtgaa gttagcgccg aaggtctgcg cattgtgctg gaagcgggta
    19501 ttcgcgagcc gatgattatg cgggccaatc aggcgctgta tgcgcaatta caccccataa
    19561 aagaatccat tttctggcgt caggttgacg gcggacatga tgcgctttgt tggcgcggtg
    19621 gcttgatgca ggggctaatc gacctctggc aaccactttt ccatgacagg agttgaatat
    19681 ggcattcagt aatcccttcg atgatccact gggagcgttt tacattttgc gcaatgcgca
    19741 ggggcaattc agtctgtggc cgcaacaatg cgccttaccg gcaggctggg acattgtgtg
    19801 tcagccgcag tcacaggcgt cctgccagca gtggctggaa gtccactggc gtactctgac
    19861 accggccaat ttcacccagt tgcaggaggc acaatgagcc agcatttacc tttggtcgcc
    19921 gcacagcccg gcatctggat ggcagaaaaa ctgtcggatt taccctccgc ctggagcgtg
    19981 gcgcattacg ttgagttaac tggagatgtt gatgcgccat tactggcccg cgcggtggtt
    20041 gtcggactag cgcaagcaga tacgttgcgg atgcgtttta cggaagataa cggcgaagtc
    20101 tggcagtggg tcgatgatgc gctgatattc gaactgccag aaattatcga cctacgaacc
    20161 aatattgatc cgcacggtac tgcgcaggca ttaatgctgg cggatttgca acaagatttg
    20221 cgcgtcgata gcggtaaacc actggtcttt catcagctga tacatgttgc ggataaccgc
    20281 tggtactggt atcagcgtta tcaccatttg ctggtcgatg gcttcagttt cccggccatt
    20341 actcgccaga tcgccaatat ttactgcaca tggctgcgtg gcgaaccaac gcctgcttcg
    20401 ccgtttacgc ctttcgctga tgtagtggaa gagtaccagc aataccgcga aagcgaagcc
    20461 tggcagcgtg atgcggcatt ctgggcagaa cagcgtcgtc aactgccgcc gcccgcgtca
    20521 ctttctccgg cacctttagc ggggcgcagc gcttcggcag atattctgcg cctgaaactg
    20581 gaatttaccg acggggaatt ccgccagctg gctacgcaac tttcaggtgt gcagcgtacc
    20641 gatttagccc ttgcgctggc agccttgtgg ctggggcgat tgtgcaatcg catggactat
    20701 gccgccggat ttatctttat gcgtcgactg ggctcggcgg cgctgacggc tagcggaccc
    20761 gtgctcaacg ttttaccgtt gggtattcac attgcggcgc aagaaacgct gccggaactg
    20821 gcaacccgac tggcggctca actgaaaaaa atgcgtcgcc atcaacgtta cgatgccgaa
    20881 caaattgtcc gtgacagcgg gcgagcggca ggtgatgaac cgctgtttgg tccggtactc
    20941 aatattaagg tatttgatta ccaactggat attcctggtg ttcaggcgca aacccatacc
    21001 ctggcaaccg gtccggttaa tgaccttgaa ctggccctgt tcccggatga acacggtgat
    21061 ttgagtattg agatcctcgc caataaacag cgttacgatg agccaacgtt aatccagcat
    21121 gctgaacgcc tgaaaatgct gattgcccag ttcgccgcgg atccggcgct gttgtgcggc
    21181 gatgtcgata ttatgctgcc aggtgagtat gcgcagctgg cgcagatcaa cgccactcag
    21241 gttgagattc cagaaaccac gctgagcgcg ctggtggcag aacaagcggc aaaaacaccg
    21301 gatgctccgg cgctggcaga tgcgcgttac ctgttcagct atcgggaaat gcgcgagcag
    21361 gtggtggcgc tggcgaatct gctgcgtgag cgcggcgtta aaccagggga cagcgtggcg
    21421 gtggcactac cgcgctcggt ctttttgacc ctggcactcc atgcaatagt tgaagctggc
    21481 gcggcctggc taccgctgga taccggctat ccggacgatc gcctgaaaat gatgctggaa
    21541 gatgcgcgtc cgtcgctgtt aattaccacc gacgatcaac tgccgcgctt tagcgatgtt
    21601 cccaatttaa caagcctttg ctataacgcc ccgcttaccc cccagggcag tgcgccgctg
    21661 caactttcac aaccgcacca cacggcttat atcatcttta cctctggctc caccggcagg
    21721 ccgaaagggg taatggtcgg gcagacggct atcgttaacc gcctgttatg gatgcaaaat
    21781 cactacccgc ttacaggcga agatgtcgtt gcccaaaaaa cgccgtgcag ttttgatgtc
    21841 tcggtgtggg agtttttctg gccgtttatc gcaggggcaa aactggtgat ggctgaaccg
    21901 gaagcgcacc gtgacccgct cgctatgcag caattctttg ccgaatatgg cgtaacgacc
    21961 acgcactttg tgccgtcgat gctggcggca tttgttgcat cgctgacgcc gcaaaccgct
    22021 cgccagaatt gcgcgacgtt gaaacaggtt ttctgtagtg gtgaggcctt accggctgat
    22081 ttatgccgcg aatggcaaca gttaaccggc gcgccgttgc ataacctgta tggcccgacg
    22141 gaagcggcgg tagatgtcag ctggtatccg gcttttggcg aggaactggc acaggtgcgc
    22201 ggcagcagtg tgccgattgg ttatccggta tggaatacgg gtctgcgtat tcttgatgcg
    22261 atgatgcatc cggtgccgcc gggtgtggcg ggtgatctct atctcaccgg catccaactg
    22321 gcgcaggggt atcttggacg acccgatctg accgccagcc gttttattgc cgatcctttt
    22381 gccccaggtg aacggatgta tcgtaccgga gacgtcgccc gctggctgga taacggcgcg
    22441 gtggagtacc tcgggcgcag tgacgatcag ctaaaaattc gcgggcagcg tatcgaactg
    22501 ggcgaaatcg atcgcgtgat gcaggcgctg ccggatgtca aacaagccgt tacccacgcc
    22561 tgtgtgatta acctggcggc agccaccggt ggtgatgcgc gtcagttggt gggctatctg
    22621 gtgtcgcaat caggtctgcc gttggatacc agcgcattac aggcacagct tcgcgaaaca
    22681 ttgccgccgc atatggtgcc ggtggttctg ctgcaacttc cacagttacc tcttagcgcc
    22741 aacggcaagc tggatcgcaa agccttaccg ttgcctgaac tgaagacaca agcgtcggga
    22801 cgtgcaccga aagcgggcag tgaaaagatt atcgccgcgg cattcgcgtc gttgctgggt
    22861 tgtgacgtgc aggatgccga tgctgatttc tttgcgctgg gcggtcattc gctactggca
    22921 atgaaactgg cagcgcagtt aagtcgacag tttgctcgtc aggtgacgcc ggggcaggtg
    22981 atggtcgcgt caaccgtcgc caaactggca acgattattg atggtgaaga ggacagctcc
    23041 cggcgcatgg gattcgaaac cattctgccg ttgcgtgaag gtaatggccc gacgctgttt
    23101 tgtttccatc cggcgtccgg ttttgcctgg cagtttagcg tgctctcgcg ttatctcgat
    23161 ccacaatggt cgattattgg cattcagtcg ccgcgccctc atggacccat gcagacagcg
    23221 acaaacctgg atgaagtctg cgaagcgcat ctggcaacgt tacttgagca acaaccgcac
    23281 ggcccttatt acctgctggg gtattccctt ggcggtacgc tggcgcaggg cattgcggcg
    23341 cggctgcgtg cccgtggcga acaggtggca tttcttggtt tgctggatac ctggccgcca
    23401 gagacgcaaa actggcagga aaaagaggct aatggtctgg acccggaagt gctggcggag
    23461 attaaccgcg aacgcgaggc cttcctggcg gcacagcagg gaagtacttc aacggagttg
    23521 tttaccacca ttgaaggcaa ctacgctgat gctgtgcgct tgttaaccac cgcccatagc
    23581 gttccgtttg atggaaaagc aacgttattt gttgctgagc gcacacttca ggaaggtatg
    23641 agtcccgaac gtgcctggtc gccgtggatt gcggagctgg atatctatcg tcaggat
//
LOCUS       DAMZII010000075        23511 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZII010000075 DAMZII010000000
VERSION     DAMZII010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.075"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            248..4708
                     /gene="gltB"
                     /locus_tag="POA77_002840"
     CDS             248..4708
                     /gene="gltB"
                     /locus_tag="POA77_002840"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI6366240.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMIFLNKDSELAAAARRTV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4721..6139
                     /gene="gltD"
                     /locus_tag="POA77_002841"
     CDS             4721..6139
                     /gene="gltD"
                     /locus_tag="POA77_002841"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDI6366241.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMHGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6323..6802
                     /locus_tag="POA77_002842"
     CDS             6323..6802
                     /locus_tag="POA77_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDI6366242.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            6819..7124
                     /locus_tag="POA77_002843"
     CDS             6819..7124
                     /locus_tag="POA77_002843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6366243.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(7200..7664)
                     /locus_tag="POA77_002844"
     CDS             complement(7200..7664)
                     /locus_tag="POA77_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI6366244.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7661..8536)
                     /gene="nanK"
                     /locus_tag="POA77_002845"
     CDS             complement(7661..8536)
                     /gene="nanK"
                     /locus_tag="POA77_002845"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDI6366245.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8533..9222)
                     /locus_tag="POA77_002846"
     CDS             complement(8533..9222)
                     /locus_tag="POA77_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI6366246.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9270..10760)
                     /gene="nanT"
                     /locus_tag="POA77_002847"
     CDS             complement(9270..10760)
                     /gene="nanT"
                     /locus_tag="POA77_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDI6366247.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(10869..11762)
                     /gene="nanA"
                     /locus_tag="POA77_002848"
     CDS             complement(10869..11762)
                     /gene="nanA"
                     /locus_tag="POA77_002848"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI6366248.1"
                     /translation="MATNLRGVMAALLTPYDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(11884..12675)
                     /gene="nanR"
                     /locus_tag="POA77_002849"
     CDS             complement(11884..12675)
                     /gene="nanR"
                     /locus_tag="POA77_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDI6366249.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            13055..14422
                     /gene="dcuC"
                     /locus_tag="POA77_002850"
     CDS             13055..14422
                     /gene="dcuC"
                     /locus_tag="POA77_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI6366250.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLDGIGVMIIMALVIAICAIVMGSGNAPFMSFASLMPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(14465..14962)
                     /gene="sspB"
                     /locus_tag="POA77_002851"
     CDS             complement(14465..14962)
                     /gene="sspB"
                     /locus_tag="POA77_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDI6366251.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(14968..15606)
                     /gene="sspA"
                     /locus_tag="POA77_002852"
     CDS             complement(14968..15606)
                     /gene="sspA"
                     /locus_tag="POA77_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDI6366252.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(16001..16393)
                     /gene="rpsI"
                     /locus_tag="POA77_002853"
     CDS             complement(16001..16393)
                     /gene="rpsI"
                     /locus_tag="POA77_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI6366253.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(16409..16837)
                     /gene="rplM"
                     /locus_tag="POA77_002854"
     CDS             complement(16409..16837)
                     /gene="rplM"
                     /locus_tag="POA77_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI6366254.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(17056..18183)
                     /gene="zapE"
                     /locus_tag="POA77_002855"
     CDS             complement(17056..18183)
                     /gene="zapE"
                     /locus_tag="POA77_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDI6366255.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRMHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            18377..18775
                     /locus_tag="POA77_002856"
     CDS             18377..18775
                     /locus_tag="POA77_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDI6366256.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            18929..20296
                     /gene="degQ"
                     /locus_tag="POA77_002857"
     CDS             18929..20296
                     /gene="degQ"
                     /locus_tag="POA77_002857"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDI6366257.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            20386..21453
                     /gene="degS"
                     /locus_tag="POA77_002858"
     CDS             20386..21453
                     /gene="degS"
                     /locus_tag="POA77_002858"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDI6366258.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(21516..22454)
                     /gene="mdh"
                     /locus_tag="POA77_002859"
     CDS             complement(21516..22454)
                     /gene="mdh"
                     /locus_tag="POA77_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI6366259.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            22889..23359
                     /gene="argR"
                     /locus_tag="POA77_002860"
     CDS             22889..23359
                     /gene="argR"
                     /locus_tag="POA77_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI6366260.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         5714 a         6223 c         6105 g         5469 t
ORIGIN      
        1 gacctgtatc agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc
       61 ctgctcatca tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa
      121 aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc gtcgccacgc
      181 tctttctgtg cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt
      241 tcacgatatg ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc
      301 ccacatagaa ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg
      361 catgcagcac cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt gcggcttgct
      421 gttacaaaaa ccggatcgct tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc
      481 caaaaactac gctgtcggga tgatcttcct gaataaagac tctgaactcg ccgctgccgc
      541 acgccgcacc gttgaagaag agctgcaacg cgaaacgttg tcgattgtgg gctggcgtga
      601 tgtccccact aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cacgcattga
      661 gcaaattttt gtgaacgccc cggcaggctg gcgtccgcgt gatatggaac gccgtctatt
      721 tatcgcccgc cgccgcatcg aaaagcgtct cgaagccgac aaagacttct acgtctgtag
      781 cctgtcgaat ctggtcaata tttataaagg tctgtgtatg ccggcggatc tgccgcgctt
      841 ttacctggat cttgcggacc tgcgcctgga atcggccatc tgcctgttcc accagcgctt
      901 ctccactaac accgtaccgc gctggccgct ggcgcagccg ttccgctatc tggcgcataa
      961 cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cctataaatt
     1021 ccagacgccg cttatccctg atctgcacga cgcagcgcca ttcgtcaacg aaaccggttc
     1081 tgactccagc tcgatggata acatgctgga actgctgctg gcaggcggga tggatatcat
     1141 ccgcgctatg cgtctgttag tgccgcccgc ctggcagaac aacccggata tggacccgga
     1201 gctgcgtgcc ttcttcgact ttaactccat gcatatggag ccgtgggatg gcccggcggg
     1261 catcgtgatg tccgacggtc gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc
     1321 ggcgcgctac gtcatcacca aagataagct catcacctgc gcctctgaag tcggtatctg
     1381 ggattatcag cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt
     1441 gatcgacacc cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag
     1501 ccgccatcca tataaagagt ggatggagaa aaacgtccgc cgactggtac cgtttgaaga
     1561 tctgcccgat gaagaagtgg gtagccgcga actggacgac gacacgcttg ccagctacca
     1621 gaaacagttt aactacagcg cggaagagct ggactccgta attcgcgtac tgggcgaaaa
     1681 cggtcaggaa gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc tctccagtca
     1741 gccgcgcatt atttacgact acttccgcca gcagtttgcc caggtgacta acccgccaat
     1801 cgacccgctg cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa
     1861 cgtcttttgc gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc cgattctgct
     1921 ctactccgat ttcaaacagc tcacgacgat gaaagaggag cactaccgcg cagatacgct
     1981 ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga
     2041 caaagccgaa aaaatggtac gtagcggcac cgtgctgctg gtgctctccg accggaatat
     2101 cgctaaagat cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc agacccgtct
     2161 ggtcgatcaa agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga
     2221 tccgcaccac ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc cataccttgc
     2281 ctatgaaacg ctgggccgcc tggtagacac ccatgcgatt gccaaagatt atcgtaccgt
     2341 gatgctcaac taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg
     2401 catctccacc atcgcctctt accgctgctc gaaactgttt gaagcggtcg gtctgcacga
     2461 tgatgtagtg ggcctgtgct tccagggggc ggtcagccgc attggtgggg cgagctttga
     2521 agacttccag caggatctgc tgaacctgtc gaaacgtgcc tggctggcgc gtaagcccat
     2581 cagccagggc ggcctgctga aatacgtcca cggcggcgaa taccacgctt acaacccgga
     2641 cgtggtgcgc acgctgcaac aggcggtaca aagcggcgag tacagcgact atcaggaata
     2701 cgcgaagctg gttaatgagc gtccggcaac cacgctgcgc gatctgctgg caattacgcc
     2761 gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt
     2821 tgataccgcc gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga
     2881 agcgatgaat agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag acccggcgcg
     2941 ctatggcact aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct tcggcgttac
     3001 tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc
     3061 gggcgaaggc ggtcagttgc cgggtgataa agtcactcct tacatcgcca aactgcgcta
     3121 ttcggtgccc ggagtgacgc tgatctcccc gccgccgcac cacgatatct actctatcga
     3181 ggacttagcg cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt
     3241 gaagctggtt tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa aagcttatgc
     3301 cgatttaatc accatcgcag gctatgacgg cggcaccggc gcaagtccgc tttcatcggt
     3361 gaaatacgca ggctgtccgt gggagctggg gcttgttgaa acccagcagg cgctggttgc
     3421 taacggcttg cgtcacaaga tccgtttgca ggtcgatggc ggcctgaaaa cgggtgtcga
     3481 tatcatcaag gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gccctatggt
     3541 ggcgctcggc tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa cgggtgtagc
     3601 aactcaggat gacaaactgc gtaagaacca ctatcacggc ctgccgttca aggttaccaa
     3661 ttactttgaa tttattgccc gtgaaacccg cgagctgatg gcacagctgg gcgtaacgcg
     3721 tctggtggat ctgattggtc gcaccgacct gctgaaagaa ctggacggtt tcaccgccaa
     3781 acagcaaaaa ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc
     3841 actctactgc accgaaaaca acccgccgtt tgataacggc ctgctgaacg cgcagttgct
     3901 gcaacaggcg aaaccgtttg tcgatgaacg ccagagcaaa accttctggt tcgatatccg
     3961 caataccgat cgctccgtgg gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga
     4021 tcaggggctg gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagttt
     4081 cggtgtatgg aacgcgggcg gcgtggaact gtacctgacc ggtgatgcca acgactatgt
     4141 cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct ccggttggtt ccgccttccg
     4201 cagccatgaa gcaagcatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct
     4261 gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg ctatcaccgt
     4321 ggtagaaggc attggcgaca acggctgtga atatatgacg ggcggtattg tctgtattct
     4381 gggtaaaacc ggcgttaact tcggcgcggg catgaccggc ggcttcgctt acgttctcga
     4441 tgaaagcggc gatttccgca aacgcgttaa cccggaactg gtcgaggtct taagcgttga
     4501 cgatctggcg atccatgaag agcatctgcg cggtcttatc accgagcatg ttcagcatac
     4561 cggttcccag cgcggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc
     4621 gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg ggtcaccgta gtcgtagcgc
     4681 agctgagttg cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca
     4741 atttatcgac ctgcagcgcg ttgatccgcc aaagaaaccg ctgaagatcc gcaaaattga
     4801 gtttgttgaa atttacgagc cgttttccga aggccaggcc aaagcgcagg ctgaccgctg
     4861 cctgtcgtgc ggcaacccat actgcgagtg gaaatgcccg gtacacaact acatcccgaa
     4921 ctggctgaag ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt cgcaccagac
     4981 caacaccctg ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc
     5041 ctgcactctg aacgatgagt ttggcgcggt gaccatcggc aacattgagc gttatatcaa
     5101 tgataaagcg ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa
     5161 aaaagtggcg attatcggcg caggcccggc aggtctggcg tgtgcggatg tcctgacgcg
     5221 taacggcgta aaagccgttg tcttcgaccg tcatccagaa attggcggac tgctgacctt
     5281 cggtattccg gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac
     5341 cggcatgggt attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga
     5401 tctgctgagt gattacgatg ccgtcttcct tggcgtcggg acttatcagt caatgcacgg
     5461 cgggctggaa aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa
     5521 caccaaacag ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg
     5581 caaacgcgtg gtggtccttg gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt
     5641 gcgccaggga gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg
     5701 ttcccgccgc gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca
     5761 gccactgggg attgaagtga acggtaacgg caaagtcagc ggcgtaaaaa tggtacgtac
     5821 cgaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg caggttccga
     5881 acatatcgtt ccggcagatg cggtgatcat ggcgtttggt ttccgtccac acaacatgga
     5941 atggctggca aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg
     6001 cagcgacaac gccttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg
     6061 tggttccgat ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag acggtattat
     6121 gaactggctg gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct
     6181 caaattctcc tttctttctg gaaagcgcct cgcataatct gaagaaaacc aatgtaatac
     6241 gctcatctgg attgttaccg gtagatgccc ggcgtatagc tgattccatt attcaacgca
     6301 gcgccatata aagggacaga taatggaatc gctctcagag agaacctcaa caggctacca
     6361 gcaaatccat gacggtatta ttcacctggt cgatagcgcc cggacggaaa cggtacgtag
     6421 cgttaacgcg ctgatgaccg cgacatactg ggaaattggc cgacgaattg tcgaatttga
     6481 acaaggtggc gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga
     6541 tttaagtcta aggtataagc gtggtttctc tgcaaaaaac ttacgccaaa tgaggctttt
     6601 ttacctcttt tttcaacatg ttgaaattcg ccagacagtg tctggcgaat taacaccatt
     6661 gggaattccg cagacaccat ctgcagaatt cccatcggca aagatttggc agacgctgtc
     6721 tgccaaattg agaggctgtt tgatatttcc acagcttttg tattgcatct cctattttcg
     6781 gtttgtatat tcatgcaaat aaacgaagga aagttgccat gatttacatt gaacagcttg
     6841 aattaatcca taaaagtggt gatgtgttgt atccagtaaa aataaccaga aaatcatcag
     6901 gaaaaactgc cttccacctt gttccattcg gcttaaataa gactcacgac cttctagagg
     6961 tggaagatgc ttcagaagca attcggctgg tgattgatga acgtcattcc attcgctgca
     7021 gtacacttac agcaactatt acgaataaaa agggcaagcg aattaagcgg acaggtatat
     7081 acagtatcaa gggtgttaac ataaaagaat acaacgtcag gtaatggcac agagcggtcc
     7141 gtcagttcag gtttggctct gtgccaaaga tgggtcaggt cacacctgat ctaataaatt
     7201 taagccatta aatcagcctt aaccttcacc accacctttt taatctcacc cggctcgcca
     7261 acaacgcatc ccggcttatg cggctctcct ggcataaaca cggcgaacat tcccggtttc
     7321 aggatgatgg tttgctcgtt ttcaatggcg ctacaaagct ggtaatcatc ttcatggtgg
     7381 aactcttcac attggcgcgc agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt
     7441 aataacagct ggatatcaat gtattgctcg tgcaattccg cttttttctc gacgggcgat
     7501 tgagtgttaa acgtcatgac attcataaaa atattgtcgc cctgtaattc gtaacgaccc
     7561 ggcgcttttt cttgcggtct ggcagctaag gccagcgtta acgcgtcctg taacgcagga
     7621 tgtaacccag cagacggtaa tgactgtact tcacccatca tcataatttt tctccctggg
     7681 ccaacagcgc agccccaagt aaacctgcat catggcggta atgcgccgcc agtaaatcaa
     7741 catgaaatgc cgctggctcc tgcgccagat acatttccac caacgccaga tacccttctg
     7801 ccagaccaac gctgccacca accaccacgc actggcaatc agttgtggct ttaatatcag
     7861 cgatcagcct tgcaagcgta cgtgcggaga gatgaatcag ttgctgcgcc tgctcatcgc
     7921 cctgcccggc gcgtgtgaaa atagttctag catccgcgcc agccagctcg ccctgcgctg
     7981 ccgccgcaat gccgcgacca gaagcaattg cttccacgca acctgtgcgt ccacagccgc
     8041 agactgggcc gtgtggatcg gcaagcgtat gcccgatgtg ccccgccaga ccgccagggc
     8101 cggtaagcag tttgccgccg ctcactacac cgccgccgac gccagtggaa acggtgataa
     8161 agaccatatc ggttatatcg ccttccagcg cctgatactc tgcccatgct gcggcctgcg
     8221 cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc cagcgttttg actaacggaa
     8281 agtgtagcaa tccaccgaga ttatgcggat tcagcgccag caaactgccg tcgcgaataa
     8341 ttccggttga agcgatggca acccgctgcg catgagcttg caacggagag actaatgcgg
     8401 ataaggcatc acgcaaggct tctggtgtct ggctggctgg cgtaggaagt tcacgacgat
     8461 cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa
     8521 tcgccagtgt ggtcatagca ccgccttttt catcgctgtg ttgtaccact gacaaatgtg
     8581 ttcaagacgc gtaatggcag aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc
     8641 tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc acccgacatc cggcgtcgct
     8701 caacgttttc accagcgcca gatccggctc ttctggcgtt tcaggcgtgg tatagccaga
     8761 aagcgtagtg ccaataattt cggctcccag cttttggcat gccaggccgt cttccggcgt
     8821 tgagcagtcg gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac
     8881 aggcaccgga cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc
     8941 cagcgcatcg acatcttcaa tataggccgt gatgcgtacc ggagaatcct ccagatcgcg
     9001 tttcacaatt ccaataatcg gcacgctcac caccgcacgc gtggcttgca gatttgccac
     9061 accttcaatg cgaatggcaa ccgcgcctgc ctgttctgcc gctaatgcca tggcggcgac
     9121 gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg caggagacaa tcaggccacc
     9181 gttagcagcg attttttgat ccagttgtgc aagtaacgac atacatcttc ccttagcgaa
     9241 aggcccggta catagaccgg gcaacaggat taacttttgg ttttgactaa atcgtttttg
     9301 gcgctgccaa atggcacggc accgctaaat ggtttgccgt cgatagcgtc atgagtacgc
     9361 aatgcttccg ggcgcagcca acgctgaacg cgggaaggca tatccagacc aatcagcagg
     9421 atcaccacaa atgtcagact gaacgatagc gatgccagcg cagtacccag atccagacgt
     9481 tgagcgatca acgcgccgat gattggggcc agtgcgccgc ccaatgcgcc aacgttgtag
     9541 gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat cagtttcggt
     9601 aagatcccgg cgatcccttg tccaagcatt tgctggaaga acagtaacag accgagcacc
     9661 cagacgtttg cgccgccaat cgcaaatacc ggaataatca acagctgcga ggccaacagg
     9721 ctacaaacgt aggctttgcg ggttcccagc cagtcaccga ggaagccgcc gacacagcac
     9781 cccaccgcag ccccaaagcc gctaaagaac agcacattgg cgacagtatg cgggttataa
     9841 gccagatcgg ttttcagata cgttggcagc agcgcctgaa tcggccatga gtagaggaaa
     9901 gcaaacaaca cgaccaccat cagcattacg cccgttggcc agcgtttgcc tgtactctgc
     9961 accataaagc tgataaagat tgcggcgcat aacagcccaa gaacagcgac gatcgcggca
    10021 ttttgcaggt taccggcgaa gcagaaccac agcgcagtag ccgccgccag tgtcattacg
    10081 atattggcaa tgcgatgttc accacggtag agaatatcca ccattgtgcg tactggtgct
    10141 ttacctgcgt gtttctcttt ccagtcttcc gcttccggga tgtttttacg cagccagaga
    10201 gcaaagatga ttggcaaaat gccgataaag aacagcgcac gccagcccca gaccggaacc
    10261 accaggctat agacctgagc ggcaacgacg gcccccacag agaagcctga aatcagaaaa
    10321 ccactggctt tgttacgcag atgttttggc cagctttcaa tgacataggt ggcgctggaa
    10381 ccgtattcac ccgccatccc catgccgatg accagacgcg cgataaacat ggtgatgtag
    10441 cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt
    10501 gccagacgac gcccgtagcg gtcacccata gcgccgagca tcaggccgcc gaaccagcga
    10561 gagataaagg ctgcggagat cagacttgcc gcctgcaccg tcgtcagccc gaattcaccc
    10621 tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc
    10681 aaccaggcag cggaaaatgc gcgccattgt gcacggttga gatggcgata ccacgggata
    10741 ttctgggttg tagtactcat tgtgagtctc ccgcggtggg cgatgcccac acgctttggt
    10801 ataaaaattg tagggtacag atgcgtttat ttcccctcac ccggtagggg cgagcgaggg
    10861 gaaacaactc acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga
    10921 tatttttcat ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata
    10981 taatggagga cagttttcag gccgcggaat acgcccgttt tgatcagtaa atcaatgact
    11041 ttgttgcact cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc
    11101 acgatcccct gatagcgcca gcccatgatg ttgtaggtac taccgatacc accatcagcg
    11161 cccgccagca gaccagaggc gaagatttcg tcataaccgt tatagagcac cagatcagga
    11221 tgttcacgac ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg
    11281 acgccaggca acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg
    11341 gctggaatgt tgtacaccac catcggcaaa ccatccgccg aatcaataat tgcccgatag
    11401 tgatcgcagt gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca
    11461 tcgaagccat aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa
    11521 ccgacgtggg cgatgagttt aatctgacct ttcgcctctt cggcgacgat ttccagtacc
    11581 tgttcacgct cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa
    11641 ccgtcgatgc cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt
    11701 gcttgttgtt ggtcataagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc
    11761 ataaatacct ctgaagtgat gcttgtctga taaacgatat acctttatac ctgttatacc
    11821 agatcaatta agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg
    11881 gcattatttc tttttgttgg tggtctgacc gaaggcgtgc caggtagcag agacgctgtt
    11941 gagatgcgat tgcaacgcac gatcggcttc gtcaggatcg tggcggcgga tcgcgtcaac
    12001 gatcgcaata tgctgttgat agctaacgtt gttatgctcg tgcagagcct gatcagccac
    12061 ggttggacgt gcggcgatta gccagtcgag cagggcaacg tggatcgcca tgaagattgg
    12121 gttacctggg atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc
    12181 gttgttatcc agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc
    12241 atcggtggcg tgttcagccg catagcgcac caggctggat tcaaagaaca gacgtaattg
    12301 ttcgaaatgg gcaatcccac caggatgaga aaggaaatct ttcgccatgc cggaaagctc
    12361 accgatgata gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg
    12421 caccagacct ttgcgtttta acgctgccag cgcttcgcgc acagaaggac gcccgacgtt
    12481 aaagaacgcc atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg
    12541 gcggatcatc tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg
    12601 gcggctacgc aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc
    12661 gttcataagg cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac
    12721 aggattgaaa gtaggggaaa atggcaggtt tttctctttg tgcctcatca ttaccataat
    12781 taacggaata attaactatt gcgaaaaatt aatgtaacgc agataaaaac atcccgtttg
    12841 aattattcat aagactattc acgagcatta tgaatattat gaatgtgttc ttacaaaata
    12901 atcataagcg catatttttt aatgaaaaac cacctcacct acaattaaaa acacgacatc
    12961 cgcaccataa atagacttgc aaaaaatata acatcattgt tttcaatctg ccgtttatgg
    13021 gattgaccgt tttcttttga cacggagttc aacaatgttc ggcataatta tatctgtcat
    13081 cgtattaatt acgatgggct atttgatcct gaaaaactac aaacctcagg tggtgctggc
    13141 tgccgcaggt atcttcctga tgatgtgcgg tgtctggtta gggttcggtg gtgtactcga
    13201 tcccgccaaa agcagcggct acttgatcgt cgatatttat aatgaaatcc tgcgcatgct
    13261 gtccaaccgc attgccggat tggggctgtc gattatggcg gtgggcggtt atgcccgcta
    13321 catggagcgc acaggagcca gtcgcgcgat ggtgagcttg ctaagccgcc cgttaaaact
    13381 cattcgctcg ccgtatatta ttctgtcggc aacttacgtc atcggccaaa tcatggcgca
    13441 gtttattacc agcgcctccg gcctgggtat gttgctgatg gtcaccttat ttccgacgct
    13501 ggtgagtctg ggagtaagtc gcctctctgc ggtggcggtt atcgcaacca cgatgtccat
    13561 tgagtggggg attctggaaa cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat
    13621 tgccacttac ttcttccact accagcttcc ggtcgcctct tgcgtcatta tctcggtggc
    13681 gatctcccac tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga
    13741 acaggcagag ctaaaagctc tcgataatgt cccgccgctc tattacgcca ttttacctgt
    13801 gatgccgtta atcttgatgc tcggctcgct gttcctcgcc cacatcgggc tgatgcagtc
    13861 agaactgcat ctggtggtgg tgatgttact gagtttgacg gtgacgatgt ttgttgagtt
    13921 cttccgcaag cataacttgc gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat
    13981 gggtacgcag tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg
    14041 cttaacgacg attggcactg ttgatgcggt tatcaggggt gcggagcatt ctggtctgga
    14101 cggtattggc gtgatgatta ttatggcgct agtcattgcc atttgtgcca ttgtgatggg
    14161 ctctggcaat gcgccgttta tgtcatttgc cagtcttatg ccgaatatcg cagccggact
    14221 acatgtacca gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc
    14281 ggtttcgccg attactgcgg tggtggtcgt tacgtcagga attgcaggcg tttcgccttt
    14341 tgcggtggtg aagcgaacag cgatccccat ggcagtcggt ttcgtggtga atatgattgc
    14401 cacaatcacg ctattttatt aagccattaa aagacaaaac aggccaccag ggcctgtttt
    14461 tgtattactt cacaacgcgt aatgccggtc gaccaccgcg tggtggctgc ggaggttcat
    14521 cgtcaggatg agtgtcatca tcgtgatctg gcttgtcgcc atcaataacc gacataacgg
    14581 tttcgttgtc tgccgatgcc tcttcatcat tcatgatgct ggtatcttca tcgtaggcag
    14641 cttcaggctc aaacatcgtg cctgcgccat tttcacgggc gtagatagcc agcacggcag
    14701 ccagcggcac agaaacctga cgcggaatgc cgccaaagcg cgcgttaaag cgtacctcat
    14761 cattcgccag ttccagattg ccgacagcac gcggcgcaat gttgagtacg atttgcccgt
    14821 cacgcgcata ttccatagga acctgcacgc cagggagcgt cacatccacc accaagtgcg
    14881 gcgtgagctg gttatccagc aaccactcat agaatgcacg cagcagatag ggacgacgtg
    14941 gtgttagctg tgacaaatcc atacagatta actccggccc agacgcattt cacgttctgc
    15001 ttcagttaaa gaagcaagga aagagtcacg ctcaaagacg cgggtcatat agcctttcag
    15061 ctctttcgca cccgggccgc tgaactcgat gcccagttgc ggcagacgcc acagcagcgg
    15121 agcaagatag caatcgacca ggctgaactc atcgctcagg aagtacggct tctgaccgaa
    15181 gaccggcgca atcgccagca gttcttcgcg cagttgctta cgtgcggcat ctgcttcaga
    15241 agctgaaccg ttgatgatgg tgttcatcag cgtgtaccag tctttttcga tgcgatgcat
    15301 gtacagacgg ctttcaccgc gagctaccgg gtaaacaggc atcagtggcg gatgcgggaa
    15361 acgctcatcc agatattcca taatgatgcg agattcccac agggtcagct cacgatccac
    15421 cagggtcgga acgctctgat tcgggttgag gtcaatcaga tcctgaggcg gattgtcctt
    15481 ttccacgtgt tcgatctcga aacttacacc tttctcagcc agcacaatgc ggacctgatg
    15541 gctatagatg tcagtaggac cggaaaacag cgtcattacc gaacgtttgt tggcagcgac
    15601 agccatgaaa acctccaggt atagtcagaa tttttactgc taccagccac caggtggcca
    15661 gtcagaagtt gtgttaccca gtaaggaacg actctctttg ttcgaaaatc aaacaaaaaa
    15721 tgagcaatac ccgacatttg ggcagaaaat tggatgatag tttaccagat tttgcgacct
    15781 ttgtggtgag tcgatgccgg aaatggggaa aaagagatgc gctttagtct gaaatagttg
    15841 acttagtccc ttattggcga tgtggttttt gttttgcctg tctgtcagac ggtagcaaaa
    15901 agcaactttc caatttttac gctgattcag aatttaacca taaaaaaacc cgccgaagcg
    15961 ggttttttcg caaattgttt tctgccggag cagaagccaa ttaacgtttg gagaactgcg
    16021 gacgacgacg tgctttacgc agaccgactt tcttacgttc aacctgacga gcgtcacgag
    16081 taacgaagcc agctttacgc agttcagaac gcagggactc gtcgtattcc atcagagcgc
    16141 gggtgatacc gtgacggatc gcaccagcct gaccagagat accaccacct ttaacggtga
    16201 tgtacaggtc cagtttctca accatgtcga ccagttccag cggctgacga actaccatgc
    16261 gagcagtttc acgaccgaag tactgttcca gagaacgttg gttgattacg attttaccgt
    16321 tgcccggttt gatgaaaacg cgagctgcgg aacttttgcg gcgaccagtg ccgtagtatt
    16381 gattttcagc cattgcctat aatcccgatt agatgtcaag aacttgcggt tgctgtgccg
    16441 cgtggttgtg ctcgttaccc gcgtaaactt tcagtttacg gaacatagca cgacccagcg
    16501 ggccttttgg caacatgcct ttaaccgcga tttcaatcac acgctcagga cggcgagcaa
    16561 tcatctcttc aaaggtcgct tgtttgatac caccgatgtg gccggtgtgg tgatagtaca
    16621 ctttgtcagt acgcttgttg ccggttacag caactttgtc agcgttcaga acgatgatgt
    16681 aatcaccggt atctacgtgc ggagtgtatt ccgctttgtg cttaccgcgc aggcgacgag
    16741 ccagttcagt agccagacgg cccagagttt taccggtcgc gtcaacaaca taccagtcgc
    16801 gttttacggt ttctggttta gctgtaaaag ttttcattaa aagcttaccc aataaatagt
    16861 tacacgttgg tgaacaccca aacgtcttca attgttgagg ttcacacgac aaagtccggc
    16921 aaacctaccc cttcgaatag cctatgccag cacacaaaaa gttttgggaa aaaaactttc
    16981 ttgtaacgtg gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt
    17041 gatttgtgac aggttttaac ccgccaaatg ctcgcgcttc agatactctt cgctttgcat
    17101 ctcttgcaga cgtgacaggc aacgctggaa ctcaaacttc agccgatcgc cctgataaat
    17161 ttcatacagc ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc
    17221 caccagcgca ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc
    17281 aaacaacatg accgtatgaa agagacgtga gagcgcaata tagtcatgct gactgcgggc
    17341 gtcgacgcac agcgtagtaa aagagaccgc cagcgtctgg ttctcgacgc ccattgtcgc
    17401 caatggccga tggttgattt ctaacgtcgg tgaattttct cgtttcgccc ccgccagcgc
    17461 caaccatagt ttatccatct gcgcccgggt ttcatcgttg agtggcgaaa gccacagatg
    17521 cgcctgagtg agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca
    17581 atgctgttta atggcatcga ttgcaggcag aaaacgcgca cgttgcaggc catttcgata
    17641 aagttcatcc ggcggaatat ttgacgtcgc taccagggta atgccgcggg cgaacagggc
    17701 tttcatcaga ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa attcgtcaaa
    17761 acagagcacg tcagtttcgg ctttaaagcg atcggcaata atttccagcg gatcggtctg
    17821 cccctgtaag gcagttagct cttcgtgcat ccgcagcata aaacggtgaa agtgcaggcg
    17881 ctgtttccgc tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg
    17941 tcctacaccg ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt
    18001 accccacagc ttaccgaccc gcgccattag cccactcgtc ctgggggctg gtggcctgct
    18061 attgatgagt tcttgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc
    18121 gggttgatgg ctgccttcat tgagcgcctt caggtattgc gatgttgggg taacgctttg
    18181 catgatctta ttgttattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa
    18241 aaggccgttc tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt
    18301 atagtggcat aatcagccgc aggcatggag cctgaagcca acaccctacg gaaacaaaag
    18361 acaacgggag atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat
    18421 tattggtgct gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca
    18481 gtacgaactg gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca
    18541 ctttgcccgc agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca
    18601 gcacatggcg aaaagctcca gcagcctgct gccggaactg tctgctgaag caaacccgtt
    18661 ccgtaatcgt ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg
    18721 cgactattct gaaggcgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat
    18781 ttttcgggcg cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg
    18841 gtgtctcatt gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc
    18901 ttatcattca ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt
    18961 agcgttaagt gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg
    19021 ccaggttgcc gatcaggccc ctctccccag tctggctcca atgctggaaa aagtgcttcc
    19081 ggcagtggtg agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga
    19141 attcaaaaag ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt
    19201 aggttccggt gtcatcatca acgccagtaa aggctatgtg ctgaccaaca accatgtgat
    19261 taatcaggca cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact
    19321 gattggtagc gatgaccaga gcgatatcgc cctgttacaa attcaaaacc cgagcaaatt
    19381 aacgcaaatc gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtggcggt
    19441 cggtaaccca tttggccttg ggcaaaccgc cacctctggc attgtttccg cattaggacg
    19501 cagcgggttg aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg
    19561 cggtaactcc ggcggtgcac tattaaacct taacggtgag ttaattggca tcaacactgc
    19621 aatccttgcg cctggcggcg ggagcgtcgg gattggattt gccatcccca gtaatatggc
    19681 gcgaacactg gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt tgttaggcat
    19741 caaaggcacc gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg
    19801 cgcgtttgtc agcgaagtgt tgccaggttc tggctcggca aaagcgggcg tcaaagcggg
    19861 cgatattatt accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg
    19921 tatcgcgact acagagccgg gcacgaaagt gaagcttggc ctgctgcgta acggcaaacc
    19981 actggaagta gaagtgacgc ttgataccag cacctcttcg tcggccagcg ctgaaatgat
    20041 cacgccagcg ctggaaggtg caacgttgag cgatggtcag ctaaaagatg gcggcaaagg
    20101 tattaagatc gatgaagttg tcaaaggaag cccagctgct caggctggct tgcaaaaaga
    20161 cgatgtgatc attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt
    20221 gctggcggca aaaccggcca tcatcgccct gcaaattgta cgcggcaatg aaagcatcta
    20281 tctgctgatg cgttaatgtc gtaaaccggg catcaggctt acgtgtgatg tccggttaac
    20341 tcgtggtatg ctgctgccgt tccctttttt aatgacgcct ccatcatgtt tgtgaagctc
    20401 ttacgttccg ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg
    20461 ctgcgcagcc ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc
    20521 agttataatc tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta caaccgtggt
    20581 ttgaacacca actctcacaa ccagcttgag atccgcaccc tgggatccgg tgtaatcatg
    20641 gatcaacgcg gttatatcat caccaataaa cacgtcatca acgacgccga tcagatcatc
    20701 gtcgccttac aggatggtcg tgtatttgaa gcattgctgg tgggatctga ctctctaacc
    20761 gatctggcgg tacttaaaat taatgccact ggcggtttac ctaccattcc aattaatgca
    20821 cgtcgcgtac cgcacattgg cgacgtagta ctggcgatcg gtaacccgta caacctcggg
    20881 cagaccatta cccaggggat tattagtgcc acggggcgaa tcggtctgaa cccgaccggg
    20941 cggcaaaact tcctacaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg
    21001 gtgaactcgc tgggagaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat
    21061 ggcgaaacgc cggaaggtat cggctttgcg attcctttcc agttagcaac caaaattatg
    21121 gataagctga tccgcgatgg tcgcgtgatc cgcggctaca ttggtatcgg cggacgcgag
    21181 atcgcaccac tgcacgcgca gagcggtggt atagatcaac tgcaagggat cgtggttaat
    21241 gaagtgtcac ctgacggccc agcggcgaat gcgggtattc aggtcaacga tctgattatt
    21301 tcggtggata acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt
    21361 cgccctggtt cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt aacgctgcag
    21421 gtcaccattc aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca aaaaaccgga
    21481 gtctgtgctc cggtttttta ttattcgcta attaattact tattaacgaa ctcttcgccc
    21541 agggcgatat ctttcttcag cgtatccagc ataccttcca gtgcgctctg ttcaaatgcg
    21601 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
    21661 gagaagaaac gggcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt
    21721 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
    21781 agggttgcag acccaccacc ggctttcgct tcaaccactt cagtacctgc gttctggata
    21841 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
    21901 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
    21961 tgtttgcctt tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg
    22021 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
    22081 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
    22141 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
    22201 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
    22261 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
    22321 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
    22381 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
    22441 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
    22501 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
    22561 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
    22621 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
    22681 cagccaaaac aacatcaatt tgataacaat taatttactt ttaagcagaa tttgcatgcc
    22741 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
    22801 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
    22861 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
    22921 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
    22981 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
    23041 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
    23101 tgaactgggt gtaccaacca cctccagtcc attgaaaaat ctggtgctgg atatcgacta
    23161 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
    23221 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
    23281 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcaa ttttagagct
    23341 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cgtcggggaa aatgttgctt
    23401 atccctctca ttccccttct ttctcctgcg atacatttaa cgaatagtgc gttttaccgc
    23461 gacacatcat tcacaaaatg aatacatacg gtaaaaaaag cacattatgc a
//
LOCUS       DAMZII010000076        22843 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZII010000076 DAMZII010000000
VERSION     DAMZII010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.076"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            254..538
                     /gene="traQ"
                     /locus_tag="POA77_002861"
     CDS             254..538
                     /gene="traQ"
                     /locus_tag="POA77_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDI6366261.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            525..1070
                     /gene="trbB"
                     /locus_tag="POA77_002862"
     CDS             525..1070
                     /gene="trbB"
                     /locus_tag="POA77_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDI6366262.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FSYTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARVDTVLQMYGGKKGAK"
     gene            1000..1341
                     /gene="trbJ"
                     /locus_tag="POA77_002863"
     CDS             1000..1341
                     /gene="trbJ"
                     /locus_tag="POA77_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDI6366263.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESS
                     EISEGTYNERK"
     gene            1295..1708
                     /gene="trbF"
                     /locus_tag="POA77_002864"
     CDS             1295..1708
                     /gene="trbF"
                     /locus_tag="POA77_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDI6366264.1"
                     /translation="MNHRKYQRELIMRENKSNPELKIRSTERDYKYISRITGRYAGLS
                     LVFLTAGIVLWTVMDIIFDACIDSWKADPELSNSSYMWNILIYAIPYALYALAAGFLV
                     TFFSVPNVRINIRKYRDIPAEMSYAPGEHIKGGQE"
     gene            1708..3081
                     /locus_tag="POA77_002865"
     CDS             1708..3081
                     /locus_tag="POA77_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDI6366265.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTR"
     gene            3078..5900
                     /gene="traG"
                     /locus_tag="POA77_002866"
     CDS             3078..5900
                     /gene="traG"
                     /locus_tag="POA77_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDI6366266.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPLLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAVAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            5916..6413
                     /locus_tag="POA77_002867"
     CDS             5916..6413
                     /locus_tag="POA77_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HDI6366267.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRDVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRQVSGCI
                     SIKKWGQNQLKDQYDGSEKLDFGGSEQTPTIYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            6445..7176
                     /locus_tag="POA77_002868"
     CDS             6445..7176
                     /locus_tag="POA77_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDI6366268.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            7429..9654
                     /gene="traD"
                     /locus_tag="POA77_002869"
     CDS             7429..9654
                     /gene="traD"
                     /locus_tag="POA77_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDI6366269.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEM
                     EQQLPPGISESGEVVDMAVYEAWQREQNPDIQQKMQRREEVNINVHRERGEDVEPGDD
                     F"
     gene            complement(9663..10061)
                     /gene="vapC"
                     /locus_tag="POA77_002870"
     CDS             complement(9663..10061)
                     /gene="vapC"
                     /locus_tag="POA77_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDI6366270.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(10061..10288)
                     /gene="vapB"
                     /locus_tag="POA77_002871"
     CDS             complement(10061..10288)
                     /gene="vapB"
                     /locus_tag="POA77_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDI6366271.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            10370..15640
                     /gene="traI"
                     /locus_tag="POA77_002872"
     CDS             10370..15640
                     /gene="traI"
                     /locus_tag="POA77_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDI6366272.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GLPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFIDLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRECGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMTGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPRTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            15660..16406
                     /gene="traX"
                     /locus_tag="POA77_002873"
     CDS             15660..16406
                     /gene="traX"
                     /locus_tag="POA77_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDI6366273.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            16465..17325
                     /locus_tag="POA77_002874"
     CDS             16465..17325
                     /locus_tag="POA77_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6366274.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYKGAFFERVAA
                     VQTQWFKKHL"
     gene            17428..17988
                     /locus_tag="POA77_002875"
     CDS             17428..17988
                     /locus_tag="POA77_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDI6366275.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAGRLEKIRRQNRTKAELQAVLDGK"
     gene            18124..18336
                     /locus_tag="POA77_002876"
     CDS             18124..18336
                     /locus_tag="POA77_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDI6366276.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            18582..18656
                     /locus_tag="POA77_002877"
                     /pseudo
     CDS             18582..18656
                     /locus_tag="POA77_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            18960..19166
                     /locus_tag="POA77_002878"
     CDS             18960..19166
                     /locus_tag="POA77_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI6366277.1"
                     /translation="MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
     gene            19450..19707
                     /gene="repA"
                     /locus_tag="POA77_002879"
     CDS             19450..19707
                     /gene="repA"
                     /locus_tag="POA77_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDI6366278.1"
                     /translation="MSQTENAVTSSSGTKRTYRKGNPVPARERQRASLARRSNTHKAF
                     HAVIQARLKDRLSELADEEGITQAQMLEKLIESELKRRATS"
     gene            19939..20013
                     /gene="tap"
                     /locus_tag="POA77_002880"
     CDS             19939..20013
                     /gene="tap"
                     /locus_tag="POA77_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDI6366279.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCG"
     gene            20006..20488
                     /locus_tag="POA77_002881"
     CDS             20006..20488
                     /locus_tag="POA77_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366280.1"
                     /translation="MANHETHSVIAGGIESDLHIDSSKYPHPFCSLLQKRAHFDSFKH
                     LIFIRGLLAIAHGKRKNKISASMVSFYVSLVQVMCAHYIPDTNKVNLSVKELSVCSGG
                     SYTRVWRALKTLDNDLHLIAFDGGTIWFRPDMFETLRVGPDELVTARRRGNSVGGGHG
                     "
     gene            20481..21338
                     /gene="repA"
                     /locus_tag="POA77_002882"
     CDS             20481..21338
                     /gene="repA"
                     /locus_tag="POA77_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDI6366281.1"
                     /translation="MADLLQKYYSQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            22041..22232
                     /locus_tag="POA77_002883"
     CDS             22041..22232
                     /locus_tag="POA77_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366282.1"
                     /translation="MAESGYGLAGLGMDKVKSINQYRLTPGFGGFTLVPYVATLYRLP
                     CRSRGIRTIKPALNVFAKV"
     gene            22305..22556
                     /locus_tag="POA77_002884"
     CDS             22305..22556
                     /locus_tag="POA77_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6366283.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            22553..22840
                     /locus_tag="POA77_002885"
     CDS             22553..22840
                     /locus_tag="POA77_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6366284.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT         5681 a         5425 c         6481 g         5256 t
ORIGIN      
        1 atgcctgctg cgatacttaa taaacttatt acaagatagt cagggaaggc attgaccggt
       61 aatgaatgtg atgctgcata tatcagcgca ataattgccg catttcccag taacgactct
      121 cttattatgt ttctgattat ttccagtttt tctttaaaag atcgcttttc catttgtacc
      181 tctgattaat atcacacaaa cgcgaatatt acagacatga atactctcat gtaaataata
      241 accggagttt aacatgataa gtaaacgcag attctcttta ccccggcttg atattacggg
      301 aatgtgggta ttttccctgg gtgtctggtt tcatatcgtc gcccgtcttg tttacagcaa
      361 accctggatg gctttttttc tggcggagct gattgcggcc attctcgtac tgttcggcgc
      421 ataccaggtg ctggatgcat ggattgcccg tgtcagccgg gaagagcgtg aggcactgga
      481 agccaggcaa caggccatga tggaagggca gcaggagggc ggacatgtct ctcactaaat
      541 cactgctgtt caccctgttg ctgagcgccg ctgctgtgca ggcctccacc cgggatgaaa
      601 tagagcgact ctggaatccg cagggtatgg ctacgcagcc tgcacaaccg gcagcaggca
      661 cgtcagccag gacagcaaag ccggctcccc gctggttccg cctcagtaat ggcaggcagg
      721 ttaacctggc cgactggaag gtggtgctgt ttatgcaggg gcattgccct tactgtcacc
      781 agtttgaccc ggtactgaaa cagctggcac agcagtacgg cttttccgtt ttttcctaca
      841 ccctggatgg tcagggggat acggcctttc ctgaagcatt accggtgcca ccggaggtga
      901 tgcagacctt cttcccgaat atcccggtgg ccacaccgac caccttcctg gtcaacgtca
      961 acacgcttga ggcattaccg cttttacagg gggcaacgga tgccgccggt tttatggcgc
     1021 gggtggatac cgttttgcag atgtacggag gaaaaaaagg tgcgaaataa gcaggtggtg
     1081 ttactcattg ccggaatatc cggcatcgtg acgggaataa tcgtcagcct gaatatcccc
     1141 tttatccgtc aggggctttt ttatcccgcc agccctgtag aaattgtcgt ttcgctgagt
     1201 cttacctttt ctgtttctgt tgtgtttttt gtgggggcaa ttgtgggatg gatttcggtg
     1261 tctgaaatat attacagccg catgaccggt ctgaatgaat catcggaaat atcagaggga
     1321 acttataatg agagaaaata aaagtaatcc tgagctcaaa atcagaagta cagaacgaga
     1381 ttataaatac atttccagaa ttactggcag gtatgctggg ttgtctctgg tattcctgac
     1441 tgccggaatt gttttatgga cagttatgga tattattttt gatgcctgta ttgattcgtg
     1501 gaaagcggac ccggaattaa gtaattcgag ttatatgtgg aatatcctga tatatgccat
     1561 tccgtatgcg ttatatgcgc ttgcagccgg gtttcttgtt acattttttt ctgtccccaa
     1621 tgtacgaatt aatatacgga aatatcgaga tattcctgct gaaatgtcgt atgcaccggg
     1681 agaacacatt aagggagggc aagaatgatg ccacgcatta aacctcttct ggttctttgt
     1741 gccgccttac tgacggtgac gcctgcagcg tcagcggatg tgaacagcga catgaatcag
     1801 ttctttaaca agctgggatt tgcttccaat accacacagc cgggcgtctg gcaggggcag
     1861 gccgccggct atgcttacgg tggctccctg tatgcccgta ctcaggtcaa aaatgttcag
     1921 ctaatttcca tgacgctgcc ggatatcaat gctggatgtg gcggcatcga cgcctatctc
     1981 ggctcgttca gttttattaa tggtgagcag ttgcagcgtt ttgttaagca gattatgagc
     2041 aatgctgccg gttacttttt tgaccttgcc ctgcagacaa cggtgccgga aataaaaacc
     2101 gcaaaagact tcgtgcagaa aatggcaagc gacattaaca gtatgaacct cagttcctgc
     2161 caggctgcac aggggattat cggcgggctt ttcccccgga cgcaggtgtc ccagcagaaa
     2221 gtctgtcagg acattgccgg tgagagcaat atttttgctg actgggcggc ttcccggcag
     2281 ggatgtaccg ttggcgggaa atctgacagt gtcagggata aagccagcga caaggataag
     2341 gagcgggtga ccaaaaacat caacatcatg tggaatgcgc tttccaaaaa cagaatgttt
     2401 gacggcaaca aagagctgaa agaatttgtg atgacgctga ccggctcact ggtgtttggt
     2461 cccaacggcg aaatcacacc gctgtcagcc agaaccactg accgctcaat tatccgggcc
     2521 atgatggaag gcggcacagc aaaaatttat cactgcaacg attctgataa atgcctgaaa
     2581 gtggtggcag acacaccggt gactatcagc cgggataatg cactgaagtc tcagattact
     2641 aaacttctgg ccagcattca gaacaaggcg gtcagtgaca cgcctctgga tgacaaggaa
     2701 aaaggcttta tttccagtac caccatcccc gtcttcaaat acctggttga cccgcagatg
     2761 ctcggagttt ccaacagcat gatttaccag ctgacggact atatcggtta cgacatcctg
     2821 ctgcagtaca ttcaggagct gatacagcag gcccgggcga tggtggccac gggaaattat
     2881 gacgaagcgg ttatcggaca tattaacgac aacatgaatg atgccacccg gcagattgcg
     2941 gcgtttcagt cacaggtgca ggtacagcag gatgcgctgc tggttgtcga tcgtcagatg
     3001 agctacatgc gtcagcagct ttccgcccgc atgctcagtc gttaccaaaa caactatcac
     3061 ttcggaggga gcacgcggtg aatgaagttt atgtgattgc cggtggtgag tggttgcgga
     3121 ataacctgaa cgccattgcc gcctttatgg gaaccaggac gtgggattcc attgaaaaaa
     3181 ttgcgctcac attgtctgtt ctcgcggtgg ccgtaatgtg ggtacagcgg cacaacgtga
     3241 tggatttgct gggctgggtg gccgtatttg tgcttatcag cctgctggtt aatgtccgca
     3301 catcggtgca gattattgat aacagtgacc tggtcaaagt tcaccgggtg gataatgtgc
     3361 cggtcggact ggcgatgcca ctttcactga cgacccgtat cgggcatgca atggtggcca
     3421 gttacgagat gatcttcacg caaccggaca gtgtcaccta cagcaaaacg gggatgctgt
     3481 ttggcgcaga actggtatca aaaagcaccg actttttgtc ccgcaatccg gaaatcgcca
     3541 atcttttcca ggattatgtg cagaactgtg ttatggggga tatttacctg aaccacaaat
     3601 acacgctgga ggagctgatg gcctccgctg atccctacac gctgattttt tcccgcccca
     3661 gcccgctgcg gggcgtttat gacagcaata ataatttcgt aacctgtaag gacgcgtcgg
     3721 tttcgctgaa agacaagctg aatctggaca cacagagtgg cggaaaaacc tggcattatt
     3781 atgcccagca gctatttggt ggacgccctg atcctaacct gctgttcagt acgctgattg
     3841 gcgacagtta cagttacttc tatggctcaa gtaaatcagc cagccagatc atccgccaga
     3901 atgtcaccat caatgcgctg aaggagggga tcaccagtta tgccgcacgt aatggtgatt
     3961 cggccagcct ggtgaatctg gccaccacgt catcgatgga gaaacaacgt ctggcacatg
     4021 tctccatcgg tcatgtggcg atgcggacac tgcccatgac gcagaccatc ctgacgggga
     4081 tcgccatcgg tattttcccg ctcctggtac tggcggctgt attcaacaaa ctgacgctgt
     4141 ctgtcctgaa aggttatgtg tttgccctga tgtggctgca gtcatggcca ctcttgtacg
     4201 ccattctgaa cagtgccatg acattttatg cgaaacagaa tggtgcgccg gtggtgctct
     4261 ctgaaatttc acagatacaa ctgaaatact ccgatctggc ttccactgcc gggtatcttt
     4321 ccatgatgat cccgccatta tcctggatga tggtcagggg gcttggggcc ggattttcca
     4381 gtgtgtacag ccatttcgct tcctctgcta tcagcccgac tgccagtgcg gcaggtagtg
     4441 tggttgacgg taattactcc tacggcaaca tgcagacgga aaacgtgaac ggcttcagct
     4501 ggagcaccaa cagcaccacg tcgtttggtc agatgatgta ccagaccggc agtggcgcaa
     4561 ccgccacaca gacccgtgac ggtaatatgg tgatggatgc aagcggagcg atgtcccgtt
     4621 taccggtcgg tatcaatgca acgcgtcaga ttgcagtggc acaacaggaa atggcccggg
     4681 aggcgtcgaa cagagcagaa agtgccctgc atggtttcag cagcagtatt gccagtgcct
     4741 ggaacacgct cagtcagttt ggttctaacc gggggagcag tgactctgtc accagtggtg
     4801 ctgacagtac gatgagtgca caggactcca tgatggccag ccgtatgcgc agtgcagtgg
     4861 aaagctatgc gaaggcgcat aatatcagta atgagcaggc gactcaggaa ctggcatcca
     4921 gaagtacgag agcttctgcc gggatgtatg gagatgctca tgctgaatgg ggggttaaac
     4981 ctaaaattct tggtgttggt ggaggattag gcgttagagg tggtggtcgg gctggcattg
     5041 actggagtga tgatgatgcg catcaggcca gcagtggttc acgggccagc tatgatgctc
     5101 gtcatgatat tgacgctaaa gccagtaagg actttaaaga ggccagcgat tactttacca
     5161 gtcgcaaagt cagtgaatcc ggcagccata ctgacaataa tgctgattcc cgggtggatc
     5221 aactgtctgc agccctgaat tcagcgaaac agagttacga ccagtacacg acgaatatga
     5281 cccgcagcca tgaatatgct gaaatggcat cccgtactga aagcatgagt gggcagatga
     5341 gcgaagattt gtcgcaacag tttgcacagt atgtgatgaa acacgcaccg caggatgccg
     5401 aagctattct gacaaatacc agttcgccgg agattgcaga acgccgtcgg gcaatggcct
     5461 ggtcttttgt gcaggaacag atacagcctg gtgttgataa tgcctggcgg gaatcccgtg
     5521 gcgatattgg taaaggaatg gagagcgtac cttcgggggg aggcagtcag gatattattg
     5581 ctgaccatca gggacatcag gccattattg agcaaagaac gcaggacagt aatatccgta
     5641 atgatgtaaa acatcaggtt gataatatgg tcacagaata taaagggaat ataggtgata
     5701 ctcagaacag catacgtggc gaggaaaata ttgtaagagg acagtattct gaactgcaga
     5761 atcaccataa aacagaggca ttatctcaga ataataaata taatgaagaa aagtcggctc
     5821 aggaaagaat gcccggggct gacagtccac aggaactgat gaaaagggca aaggagtatc
     5881 aggataagta taagcaataa tactgtgttt gggagatgat aatgagatgt ttagcccata
     5941 ttgctctggt aacagtgata cagtttatcg cctgttatct ggctgattgg gggaatgccg
     6001 aaacagtttt tatgctgttc ttcatagtgc tatggcaggg actgtttatc tggttgttct
     6061 ctcaaatacg aaaaaaacgg gatgtgtcgg atgagtttaa gtttagtaaa ggcgtttggt
     6121 atataacgat gccagtatct tccttactct ctccgttatt aagcctgatg gtttttatca
     6181 ttggtacact atacgaatta aggcaagtta gtggttgcat aagcataaaa aaatgggggc
     6241 aaaatcaact caaagaccaa tatgatggga gtgaaaagtt agacttcggg ggcagtgaac
     6301 agaccccaac gatctactat aacccttcaa ccggatatcc tatgcatgga ggctttgatt
     6361 cagcgggaaa tacttttggg actcgttggc aggactatta tgacaggcaa taagaaaact
     6421 gtagtttgta aaaaaggtaa tttgatgaaa acaaaaaaat tgatgatggt tacactggtc
     6481 agttccactc tggccctttc agggtgtggt gcgatgagca cagcaatcaa gaagcgtaac
     6541 cttgaggtga agactcagat gagtgagacc atctggcttg aacccgccag cgaacgcacg
     6601 gtatttctgc agatcaaaaa cacgtctgat aaagacatga gtgggctgca gggcaaaatt
     6661 gctgatgctg tgaaagcaaa agggtatcag gtggtgactt ctccggataa agcctactac
     6721 tggattcagg cgaatgtgct gaaggccgat aagatggatc tgcgggagtc tcagggatgg
     6781 ctgaaccgtg gttatgaagg cgcagcagtt ggtgcagcgt taggtgccgg tatcaccgga
     6841 tataattcca attctgccgg tgccacactc ggtgtgggcc ttgctgccgg tctggtgggt
     6901 atggctgcag atgcgatggt ggaagatgtg aactatacca tgatcacgga tgtgcagatt
     6961 gcagagcgta ctaaggcaac ggtgacaacg gataatgttg ccgccctgcg tcagggcaca
     7021 tcaggtgcga aaattcagac cagtactgaa acaggtaacc agcataaata ccagacccgt
     7081 gtggtctcaa atgcgaacaa ggttaacctg aaatttgaag aggcgaagcc tgttctcgaa
     7141 gaccagctgg ccaaatcaat cgcaaatatt ctctgattat cttccggagg ctggtctgac
     7201 cggcctcctg actgacgcct cgctttgctc gttgtccaac cccggggaga aataaaataa
     7261 attttatttc tccccggcgt ttccgaaata aatatgaaaa cactgagcgg ttgtgcaaat
     7321 tctttgtgcg accgtgaagt gttgccggcg aagccggaat ttaacggtta ttaatctttt
     7381 catcattaat aatcagtccg actgactttc tttcttcaga atatcatcat gagttttaac
     7441 gcaaaggata tgacccaggg cggtcagatt gcgtccatgc gtatccgcat gttcagtcag
     7501 atcgccaata tcatacttta ctgcctgttt atttttttct ggatcctagt tggtctggtt
     7561 ttatgggtaa aaataagctg gcagacgttt gtgaatggct gcatttactg gtggtgcacc
     7621 acgctggaag gcatgcggga tttaatcagg tcccagccgg tctatgagat ccagtattac
     7681 ggcaaaacct tccggatgaa cgcggctcag gtactgcatg ataaatatat gatctggtgc
     7741 ggtgagcagt tatggtcggc attcgttctg gcgtctgtcg tggccctggt tatctgcctg
     7801 atcaccttct ttgttgtctc ctggattctg gggcgtcagg gtaaacaaca gagcgaaaat
     7861 gaaatcacag gtggtcgtca gctgactgac aatccgaaag aggtggccag gatgctgaaa
     7921 aaagatggca aggattccga tatccggatt ggcgatctgc cgattatccg ggattctgaa
     7981 atccagaact tctgtctgca cggcactgtc ggggccggta agtcggaggt tatccgtcgt
     8041 ctggccaact acgcccgtca gcgtggtgat atggtggtga tttatgaccg ttcgggggaa
     8101 tttgttaaaa gttactatga tccctccatc gataaaatcc tgaatccgct ggatgcacgc
     8161 tgtgctgcct gggatttgtg gaaggagtgc ctgacacagc cagattttga taataccgca
     8221 aatactctga tcccgatggg gacaaaagag gacccgttct ggcagggttc aggacgtacc
     8281 atttttgcgg aagcggcata cctgatgcgt aatgacccca accgcagcta cagcaaactg
     8341 gtggacacac tgctttccat caaaattgaa aagctgcgta ccttcctgcg taattcaccg
     8401 gcggccaacc tggtggaaga gaaaatcgag aaaacggcca tatccatccg tgctgtgctg
     8461 accaactacg tgaaagccat ccgttacctg caggggattg agcataacgg tgagcccttc
     8521 accatccgtg actggatgcg tggtgtccgg gaagatcaga aaaacggctg gctgtttatt
     8581 tcgtcgaacg ccgacaccca tgcctccctg aagccggtga tctccatgtg gctgtccatt
     8641 gctattcgtg gtctgctggc aatgggagaa aaccgtaacc gtcgtgtgtg gtttttctgt
     8701 gacgagttac ccacgttaca caaactgccg gacctggtgg agatcctgcc ggaagcccgt
     8761 aagttcggtg gctgttatgt gtttggtatc cagtcctatg cccagctgga agatatctac
     8821 ggtgagaaag cggctgccac gctgtttgac gtcatgaaca cccgtgcctt tttccgttct
     8881 cccagccata agattgcaga gttcgctgcc ggtgaaattg gtgagaaaga gcacctgaaa
     8941 gccagcgagc agtattccta cggtgctgat ccggtacgtg acggggtatc gaccggtaag
     9001 gatatggagc gtcagacgct ggtcagttat tccgacattc agtctctgcc ggatctgacc
     9061 tgttatgtca ccctgcccgg accgtatccg gcagtaaaac tctccctgaa atatcagaca
     9121 cgaccgaagg tcgctccgga gtttattccg cgtgacatca acccggaaat ggagaaccgt
     9181 ctgagtgccg tacttgccgc aagggaagca gaaggtcgtc agatggccag cctcttcgaa
     9241 ccggatgtcc cggaggttgt ttccggagaa gacgtgactc aggctgaaca gccgcaacag
     9301 ccgcaacagc cgcaacagcc gcaacagccg caacagccgc aacagccgca acagccgcaa
     9361 cagccgcaac agccggtgtc tcctgccatc aacgataaga agtcagatgc aggtgtgaat
     9421 gttccggcag gggggatcga gcaggagctg aaaatgaaac cggaagaaga gatggaacag
     9481 caactgccac ccgggatcag tgaatccggt gaagtggtgg atatggccgt ttatgaggca
     9541 tggcaacggg agcagaatcc ggatatccag cagaagatgc agcgtcgtga agaggtgaac
     9601 atcaatgtgc accgggagcg cggggaggat gttgagccgg gagatgattt ctgatgaaca
     9661 ggtcagctcc agtcttcaat tctcagaccg tccacacgtt caaattcccg ggtgttatta
     9721 gtcacaataa tcagtccccg actgcgggca tgacctgcga tcatttgatc aaatggcccg
     9781 acagggcgtc cctgacgggc aagttctgct cttatctggc cgctgtgtgt ggctgcggca
     9841 gcgtcgtaat ccagaacatc aatgcgggaa acaaatccct cgatcacagc gagattacgt
     9901 tcaggcatct ggcttttttc tgcaccatat atcagctcca tcagggtgac cgaactgata
     9961 cacattctcc cctggttcag gttaaaacgc tccctgacgc tggcgggttt gttctttatc
    10021 gtgaaaatgc agatgttggt atcgagcata aacttcagca tcagaatgac tccctttcct
    10081 gcatgccggg ctgttccctg ttatccataa aatcggcgct gacgttgtgt ccgtcgaacc
    10141 attcgtccca tgtctctccg gctggcgtaa tgattctggt tcgtccgaca gcaatcactt
    10201 caacgcgttt tacgttttcc ggcaatgcaa ccgcttttgg cagtctgacc gcctggctgc
    10261 ggttgctgaa aaatacggtg gtttccatta tttgcctcct tatgtggata tgtcttgtgt
    10321 atatccattg tgggattgat accgggatat gtcaaaggga tatacgttta tgatgagtat
    10381 cgcgcaggtc agatcggccg gaagtgccgg taactattat accgacaagg ataattacta
    10441 tgtgctgggc agcatgggag aacgctgggc cggcaggggg gctgaacagc tggggctgca
    10501 gggcagcgtc gataaggatg tttttacccg tcttctggag gggaagctgc cggacggagc
    10561 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
    10621 ctccgccccc aaaagtgtct ccgtgatggc catgttaggc ggtgataaac gtcttattga
    10681 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
    10741 ggttatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
    10801 taaccacgac accagtcgcg atcaggaacc acagttacac acacatgcgg tagtggccaa
    10861 tgtcacgcag cataacggcg agtggaagac attgagcagt gacaaagtgg ggaaaacggg
    10921 gttcattgag aatgtgtacg ctaatcagat tgccttcggc aggctctacc gggaaaaact
    10981 gaaagagcag gttgaggcgc tgggctatga aacggaagtg gttggtaagc acggtatgtg
    11041 ggagatgccg ggcgtaccgg tggaggcctt ctccggacgc tcacaggcta tccgggaggc
    11101 cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
    11161 caaacagcac gtcgatcctg aagtcagaat ggctgaatgg atgcagacgc tgaaggaaac
    11221 cgggttcgac atccgggcgt atcgtgacgc ggcggaccaa cgggcggaga cccgcacgca
    11281 gacgcccgga cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat
    11341 tgccggatta agtgaacgca aagtgcagtt tacgtacacg gacgtgctgg ccagaacggt
    11401 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
    11461 catcagccgt gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat
    11521 tcatgtgctc gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg
    11581 ggtgaccgta catccggaga aaagtgtccc ccggacggcc ggttacagcg atgcggtcag
    11641 tgttctggca caggatcgcc cgtcgctggc tattgtgtcc gggcagggcg gtgcagccgg
    11701 gcagcgtgag cgggtggcgg agctggtcat gatggcccgg gagcaggggc gggaggtgca
    11761 gattatcgcc gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
    11821 tgagctgata accggacgtc gtcagctgca ggagggcatg gccttcacgc cgggcaatac
    11881 tgttatcgtt gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggacgg
    11941 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
    12001 cagtgcactg atggccatga aagatgccgg ggtgaacaca tatcgctggc aggggggaga
    12061 acagcggccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
    12121 aggagatttt gctgccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
    12181 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct
    12241 cggactcccg gaggtgacca tgactgccct ttcaccggtc tggctggaca gccggagccg
    12301 ttatctgcgg gatatgtacc gtccggggat ggtgatggag cagtggaacc cggagacacg
    12361 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
    12421 ggatgcgcag ggtgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
    12481 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
    12541 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
    12601 gatgacggtt gttgtgccgg ggcgggctga accggcctcc ctgcctgtga gcgattcacc
    12661 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga
    12721 cagtgcgaag gtttttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
    12781 tctggcccgc agcggtcgtg atgtccggct gtattcttca ctggatgaaa cccgtactgc
    12841 ggaaaaactt gcccgccatc cctcctttac ggtggtttct gagcagataa aggcgcgtgc
    12901 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
    12961 acagcaggct attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
    13021 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccagtt ttatcgacct
    13081 gggaggggaa atcaatgcgc agataaaacg tggtgattta ctgtatgtgg atgtggcaaa
    13141 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
    13201 tcgccatatt ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga
    13261 actcatggag aaactgacat caggacagcg tgccgctacc cgcatgatac tggaaacgtc
    13321 cgaccgtttc acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc
    13381 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgtgtcg ttgggctggg
    13441 tcccacgcac cgcgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc
    13501 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
    13561 cacgctgttc ctgctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata
    13621 cgccctgatt gcggccggtg gcggtcgtgc cgtggccagt ggtgacacgg accagttgca
    13681 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
    13741 catcatgaag gagattgtgc gccagacgcc ggagctgcgg gaggcggtat acagcctgat
    13801 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
    13861 acgtcaggag ggcgcatggg taccggagca ctccgtgacg gagttcagtc acagccagga
    13921 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggtgaggctt ttccggatat
    13981 tcccatgaca ctgtatgaag ccattgtccg cgattatacc ggcaggacgc cggaagcacg
    14041 ggagcagacg ctgattgtca cgcatctgaa tgaggaccgg cgcgtactga acagcatgat
    14101 tcatgatgca cgggaaaagg ccggtgagct gggaaaagag caggtcatgg tgcctgtcct
    14161 gaacacagcg aatatacgcg acggggagtt gcgtcgtctc tccacctggg agacacatcg
    14221 ggacgcactt gccctggtgg ataatgtgta tcaccggatt gccggtatca gcaaggatga
    14281 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
    14341 ggtggctgaa ggcgtcacac tgtacactcc ggacaccatc cgggtgggaa ccggtgaccg
    14401 gatgcgcttc acgaagagtg accgggagtg cggttatgtg gccaacagcg tctggacggt
    14461 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
    14521 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ccgcccacgg
    14581 tgcgcagggg gcaagtgaaa cctttgccat tgcgcttgaa ggcacggaag gtaaccggaa
    14641 acagatgacc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
    14701 ggtgtatacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
    14761 aactgcccac gatgtgctag agccgaaatc ggaccgggag gtcatgaatg cagagcggct
    14821 gttcagtacg gcgcgggagc tgcgggacgt ggtggccggc cgtgccgttc tccgtcaggc
    14881 agggctggcc gggggagaca gtcctgcacg gtttattgca cccgggcgca aatatccgca
    14941 gccgtatgtg gcactgccgg cttttgaccg taacggcaag tcagctggta tctggctgaa
    15001 cccgctgacc acggatgacg gaaacgggct gcggggcttc agtggtgaag gccgggtgaa
    15061 aggcagcggg gatgcgcagt tcgtggccct gcagggcagc cgtaacggag agagcctgct
    15121 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aatcctgaca gcggtgtggt
    15181 ggtaagaatc gccggtgaag gtcgtccgtg gaatcccaga accatcaccg gtggtcgcgt
    15241 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
    15301 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgccg
    15361 tgcggatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
    15421 agagcaggct gtcagggata ttgccgggct ggagcgtgac cggtctgcca tatctgaacg
    15481 ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg
    15541 ggaggttgcc cgggaaaatc tgctgcagga gcgactgcag cagatggagc gggatatggt
    15601 tcgtgacctg cagaaagaga aaaccctggg cggagactga cacaggaaga taaacgctga
    15661 tgacaacgga taacacgaac acaacacgta atgattcact ggctgcccgg accgatacct
    15721 ggttgcagtc gttgctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
    15781 tggtgctgat ggttctggac catgcaaacc gcattctgca tctggaccag tcgtggatgt
    15841 ttctggtcgg gcgtggggcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
    15901 gtcatacgca tatccggcag gaggccatta accggctgtg gggatgggcg gtgattgccc
    15961 agttcgcata ttatctggcc ggctttcctt ggtatgaggg gaatatcctg tttgcctttg
    16021 cagttgcggc acaggtgctg acatggtgtg aaacgcgcac ctggtggcgt tccgcagaga
    16081 ccatgttgct tctggcaatg tggttgcctt tctccggcac cagttacggc attgccgggc
    16141 tgctgatgct ggcagtcagc cacaggctgt accgggcgga agacagaatg gaacgtctgg
    16201 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
    16261 cggcggtggc cggccttgtg atgacggtgc tgactgttgg tctggtgtcc tgtacaggga
    16321 aatcactacc ccgtttctgg tacggggatt ttttcccgac gttctatgcc tgtcatctta
    16381 ctgtgctggg cgttctggct gtgtgactgg tatgatgctg gcgctgattc tgcttattaa
    16441 taaacaacat aacacaggat atttatgaaa attacggatc acaaactccc tgaaggtatt
    16501 gccctgacat ttcgtgtccc ggaaggaaat ataaaacatc ctttgattct cttatgccat
    16561 ggattttgcg gtatccggaa tgttctgctg ccttcctttg cgaatgcgtt tacagaagcc
    16621 gggtttgcaa cgattacttt tgattatcgt ggattcggtg agagtgaagg ggaacgaggt
    16681 cgtctggttc cggcaatgca gaccgaagat attatttcag ttatcaattg ggcagaaaag
    16741 caggtatgta ttgataatca gcgtatagga ttatggggaa catcgctggg tgggtgtcat
    16801 gtattcaatg cagctgcgca ggataaaaga gtgaaaagta ttgtcagcca gctggctttt
    16861 gctgacggtg aggttctggt taccggagaa atgaatgaac tggagaaagc ttcttttctg
    16921 tccacgctga ataaaatggc agaaaagaag aaaaataccg ggaaagagat gtttgtgggg
    16981 gttacccggg tactgagtga taatgaatca aaggtattct tcgagaaggt aaaaggtcag
    17041 tatcctgaaa tggatataaa aatacctttc cttacggtta tggagacgtt gcaatataaa
    17101 ccggcagaga gcgcagccaa agtacagtgc ccagttttgg ttgttattgc cggccaggat
    17161 agtgttaatc caccagaaca gggaagagcc ttatatgatg ctgttgcttc tggtactaag
    17221 gaattatacg aggaagctga cgcatgccat tatgatattt ataaaggcgc gttttttgaa
    17281 cgtgtggcgg cagtacagac gcaatggttt aaaaaacatc tttaattgtc ggtattaata
    17341 tgtcagaggt cgttgagatc agcttaatat catagtgagt agtttcaacg acctggagga
    17401 catattttca caaaggtcag ggcaatcatg acagagcaga aacgaccggt actgacactg
    17461 aaacggaaaa cggaagggga aactccgacc cggagccgga aaaccatcat caatgtcacc
    17521 acgccaccaa aatggaaggt gaaaaagcag aaactggcgg agaaggctgc ccgggaagca
    17581 gagctggcgg cgaagaaagc acaggccaga caggcgctgt ccatttatct gacgctgccc
    17641 tcgctggatg aggcggtgaa cactctgaaa ccttggtggc cgggattgtt tgacggtaac
    17701 acgccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg
    17761 aatatcccgc tctcgcataa aaaactgcgc cgggcgctga aggccatcac ccgttcagaa
    17821 agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg gtatgtgacg
    17881 gagcatattt ctcaggagga ggaagcgtat gcggcagggc gtttggagaa aattcgccgc
    17941 cagaaccgga caaaggcaga acttcaggcc gtgcttgatg ggaaataaaa aaagcctccc
    18001 cggaaaccgg ggaggctgaa acaataaggc ttaaaaggaa ataacttccg tacccgaaag
    18061 gaagccagtc cactgaatta tttaccacaa taatactgac gactaaaagg gaggagcaga
    18121 aaaatgaatg gattcagaaa cagttcacgg aacggtcagg tctggcgtta ccagcgtgcc
    18181 ggcgggcggg ccgttattct ggaggtcagt ggacgctgga tggaagcggc agaagcatgg
    18241 cgacgggctg cctgcatagc tccccggaca gactggcaac agtttgcccg gaaaagggct
    18301 gaacactgcc accggcgctg ccggggaagg gtgtaacgaa aaaagcagcc ttcattactc
    18361 caggagtcag aaggctgaaa ggagtttatg agatttgctt tttttaatat ccggtcgggc
    18421 agtatgaccg gccagttctg agtagcacag agttttttcc gtattaagtc gcgattttat
    18481 attttgtgat taatttcaca aaataaggtc ttattcaatg tgtgctgcga tattcagggg
    18541 ggctgaaata caggtgccat ttatctgata tggagaataa catgaggaaa tatattccac
    18601 tggtattatt tatcttttca tggccggtat tgtctgcaga tgttcacggg cagatttatg
    18661 caccagacag aatgaatgtt aattagttta tgaatacaac aggggctgat cagcaatcac
    18721 cggaacatcg tagctgacgc gttcgcaatg ctcagttgtc ccccccggaa acgggaaaaa
    18781 gtaagttttc ccccgctccc ggcgtttttg taacaaaaac aggcaaatgg gggtaatagt
    18841 cagcaacagg gaatgtggta ttatcgcggc gggtgtttga gcctttctgg ttcaggcaag
    18901 acgcaggtac cagaaatgcg aagaccccac tcgttaatcc attaactcgt gaggtctgca
    18961 tgaagtacct taacactact gattgtagcc tcttctttgc tgagaggtca aagtttatga
    19021 cgaaatatgc ccttatcggg ttgctcgccg tgtgcgccac ggtgttgtgt ttttcactga
    19081 tattcaggga acggttatgt gagctgaata ttcacagagg aaatacagtg gtgcaggtaa
    19141 ctctggccta cgaagcacgg aagtaagctg ccgggcgggg acggaagtcc ccgctttccg
    19201 gaagtgtgag gtatttcagg ggcagacacc ctatatacca gaaacagccg gtcccgcccg
    19261 gggccggcac cctggctaag gcatttccag cttttcagtc atttcattat caaaatcaca
    19321 ttaaacggtc gtaatcagac atgatttgtg cgccaacaca gatcatcgtc acaactttca
    19381 agtcgctgat ttcaaaaaac tgtagtattc tctgcgaaac gatccctgtt tgagaattga
    19441 ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct catcaggcac caagcgtaca
    19501 taccggaaag gtaaccctgt tccggccaga gagaggcaaa gagcttctct ggctcgcaga
    19561 agcaacactc ataaggcttt tcatgcggtt attcaggccc ggttaaaaga caggctgagt
    19621 gaactggcgg atgaggaagg tattactcag gcgcagatgc ttgaaaaact gattgaatca
    19681 gagctgaaac gcagagcgac ttcgtaaata ttcacattct tgcttatctc aggagtgagt
    19741 ggtagattgc tgatcgttta aggaattttg tggctggcca cgccgtaagg tggcagggaa
    19801 ctggttctga tgtggattta caggagccag aaaagcgaaa accccgataa tcttcttcaa
    19861 gtttggcgac tagaaagatt accggggccc cataaaaccg catagccaac aattcagcta
    19921 tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg ctcactcctt
    19981 ctgtgcattg taagtgcagg atggtgtggc taatcatgaa acacattcag taatagcggg
    20041 tgggattgaa tcagatcttc acattgattc cagcaagtat cctcacccgt tttgcagcct
    20101 tctccagaaa agggctcatt ttgactcctt caagcatctg atcttcatca gaggtttgct
    20161 tgcaatagcg catggcaaac gtaaaaataa aatcagcgcg tcgatggtta gtttttatgt
    20221 ttccctcgta caagtaatgt gcgcacacta catccctgat acgaacaaag ttaacttatc
    20281 tgttaaagag ctttcagttt gtagtggagg ttcatacact cgtgtttgga gagccctcaa
    20341 aactcttgat aatgatcttc atctcatcgc ttttgatgga ggaaccatct ggttcagacc
    20401 agatatgttc gaaactttac gtgtcggccc agacgagcta gttaccgccc gtaggagggg
    20461 gaatagtgtt ggagggggac atggctgatc tccttcaaaa atactattca caggttaaaa
    20521 atccgaatcc ggtgttcaca ccccgtgaag gtgccggaac gctgaagttc tgcgaaaaac
    20581 tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat gtggcacatg
    20641 cccgttcgaa gggactgcgt cggcgcatgc caccggtact gcgtcgccgg gctattgatg
    20701 cgctgctgca ggggctgtgt tttcactatg acccgctggc caaccgcgtc cagtgctcca
    20761 tcactacgct ggccattgag tgcggactgg cgacggagtc tgctgccgga aaactctcca
    20821 tcacccgggc ctcccgagcc ttgacgttcc ttgcagagct gggactgatt acctaccaga
    20881 cggaatatga tccgcttatc gggtgctaca ttccgaccga tatcacgttc acaccggcgt
    20941 tatttgccgc ccttgatgtg tctgaggatg cagtggttgc tgcgcgccgc agtcgtgttg
    21001 aatgggaaaa cagacagcgc aaaaagcagg ggctggatac cctgggtatg gatgaactga
    21061 tagcgaaagc ctggcgtttt gtgcgtgagc gtttccgcag ttaccagaca gagcttaagt
    21121 cccgtggaat aaagcgtgcc cgtgcgcgtc gtgatgcgaa cagggaacgt caggatatcg
    21181 tcaccctggt gaaacggcag ctgacgcgtg aaatctcgga agggcgcttc actgccaatc
    21241 gtgaggcggt aaaacgcgaa gtggagcgtc gtgtgaaaga gcgcatgatt ctgtcacgta
    21301 accgtaatta cagccggctg gccacagctt ccccctgaaa gtgacctctt ctgaataatc
    21361 cggcccgcac cggaggcatt cgcacgcctg aagcctgcca gcgaacaaaa aagcagcacc
    21421 tcatacaaaa aacaacctca tcatccacct tctggtgcat ctggcccccc ctgtttttga
    21481 tacaaaacac gcctcacaga cggggaattt tgcttatcca catttaactg caatggactt
    21541 ccccataagg ttacaaccgt tcatgtcata aagcgccagc cgccagcctt acagggtgca
    21601 atgtatcttt taaacacctg tttatatctc ctttaaacta cttaattaca ttcatttaaa
    21661 aagaaaacct attcactgcc tgtcctgtgg acagacagat atgcacctcc caccgtaaac
    21721 ggcgggcccc aaccggagcc actttagtta caacacacaa aaccaacctc cagaaaaacc
    21781 ccgaaccagc gcagaactga aaccacaaag cccctccctc ataactgaaa agcggccccg
    21841 ccccggccct tcgggccgga acagagtcgc ttttaattat gaatgttgta actaagcatt
    21901 gccgtcgctg tcagtcttct ggctggaagt attgaataca cgctcgtaag cggccctgac
    21961 ggcccgctaa cgcggagata cgccccgact tcgggtaaac cctcgtcggg accactccga
    22021 ccgcgcacag aaactctgtc atggctgaaa gcgggtatgg tctggcaggg ctggggatgg
    22081 ataaggtgaa atctatcaat cagtaccggc ttacgccggg cttcggcggt tttactcttg
    22141 tgccatatgt agctacatta taccgccttc catgccgctc acgcggcatc agaacaataa
    22201 aaccagcatt aaatgtattt gcaaaagtat gagtaataca gacttgttaa ccggtccttt
    22261 cagaggtatc tttaagactt gttaaacagt ctcaaaggta cgttatgcct aacatcattc
    22321 tgtctgacac cagtgcaagt gtcagtgaac tgaaaaaaaa tcctatggct acagtcagtg
    22381 ccggagatgg ttatcctgtc gccattctta accggaatca gccggcattc tactgtgtgc
    22441 ctgcggaact ctatgaaagg atgcttgatg cgctggatga ccaggaactg gtgaaactgg
    22501 ttactgaacg cagcaatcag ccactgcatg atgtggacct ggacagttat ttatgactta
    22561 tacagtaaaa ttcagggatg atgcgctcaa agaatggctg aaactggata aatccattca
    22621 acagcagttt gcgaaaaaac taaagaaatg cagcgaaaat cctcatattc cttccgcaaa
    22681 actgcgtgga ttaaaagact gctacaaaat caaacttcgc gcatcggggt ttcgtctggt
    22741 ctatcaggtt attgatgaca tgctaattat tgcggttgtc gcggtaggta aacgtgaacg
    22801 tagtaacgtt tataatctcg ccagtgagag aatgagatag tta
//
LOCUS       DAMZII010000077        22111 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZII010000077 DAMZII010000000
VERSION     DAMZII010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.077"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            44..1720
                     /locus_tag="POA77_002886"
     CDS             44..1720
                     /locus_tag="POA77_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDI6366285.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1853..3157)
                     /gene="gsk"
                     /locus_tag="POA77_002887"
     CDS             complement(1853..3157)
                     /gene="gsk"
                     /locus_tag="POA77_002887"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDI6366286.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3309..4268
                     /gene="aes"
                     /locus_tag="POA77_002888"
     CDS             3309..4268
                     /gene="aes"
                     /locus_tag="POA77_002888"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDI6366287.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4265..5227)
                     /gene="hemH"
                     /locus_tag="POA77_002889"
     CDS             complement(4265..5227)
                     /gene="hemH"
                     /locus_tag="POA77_002889"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI6366288.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5463..6107)
                     /gene="adk"
                     /locus_tag="POA77_002890"
     CDS             complement(5463..6107)
                     /gene="adk"
                     /locus_tag="POA77_002890"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6366289.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(6288..8162)
                     /gene="htpG"
                     /locus_tag="POA77_002891"
     CDS             complement(6288..8162)
                     /gene="htpG"
                     /locus_tag="POA77_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDI6366290.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8272..8877)
                     /gene="recR"
                     /locus_tag="POA77_002892"
     CDS             complement(8272..8877)
                     /gene="recR"
                     /locus_tag="POA77_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI6366291.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8877..9206)
                     /locus_tag="POA77_002893"
     CDS             complement(8877..9206)
                     /locus_tag="POA77_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI6366292.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9259..11190)
                     /gene="dnaX"
                     /locus_tag="POA77_002894"
     CDS             complement(9259..11190)
                     /gene="dnaX"
                     /locus_tag="POA77_002894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI6366293.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(9862..9926)
                     /gene="dnaX"
                     /locus_tag="POA77_002894"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(11319..11870)
                     /gene="apt"
                     /locus_tag="POA77_002895"
     CDS             complement(11319..11870)
                     /gene="apt"
                     /locus_tag="POA77_002895"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI6366294.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(12023..12400)
                     /locus_tag="POA77_002896"
     CDS             complement(12023..12400)
                     /locus_tag="POA77_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDI6366295.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12470..12997
                     /gene="priC"
                     /locus_tag="POA77_002897"
     CDS             12470..12997
                     /gene="priC"
                     /locus_tag="POA77_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDI6366296.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            13011..13172
                     /gene="rsmS"
                     /locus_tag="POA77_002898"
     CDS             13011..13172
                     /gene="rsmS"
                     /locus_tag="POA77_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDI6366297.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13384..16746)
                     /gene="mscK"
                     /locus_tag="POA77_002899"
     CDS             complement(13384..16746)
                     /gene="mscK"
                     /locus_tag="POA77_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDI6366298.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16874..17521)
                     /gene="acrR"
                     /locus_tag="POA77_002900"
     CDS             complement(16874..17521)
                     /gene="acrR"
                     /locus_tag="POA77_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDI6366299.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17663..18856
                     /gene="acrA"
                     /locus_tag="POA77_002901"
     CDS             17663..18856
                     /gene="acrA"
                     /locus_tag="POA77_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDI6366300.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18879..22028
                     /locus_tag="POA77_002902"
     CDS             18879..22028
                     /locus_tag="POA77_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI6366301.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5057 a         5801 c         5897 g         5356 t
ORIGIN      
        1 cccggccaaa aatggacaat aaccaccagg aaaaggagac ggaatgcatc acgccacccc
       61 gcttatcacc accattgttg gcggccttgt gctcgccttt atcctcggca tgctggccaa
      121 taaactacgt atttctcctc tggtgggata tctgttagcg ggtgtgctgg caggaccatt
      181 cactccgggc tttgttgccg ataccaagct tgcgccggaa ctggctgaac tgggcgtcat
      241 tctgttgatg tttggcgtcg gtttgcactt ttcgctgaag gatttgatgg cggtaaaggc
      301 catcgccatt cccggtgcga tcgcccagat agccgtggcg acgctgctgg gtatggcgct
      361 ctctgccgtg ctgggctggt cgttaatgac cggtatcgtg ttcggtttat gtctttccac
      421 cgccagtacc gtggtgttac tgcgcgcact tgaagaacgg caattaattg acagtcagcg
      481 tgggcaaatc gccatcggtt ggttgattgt ggaagacctg gtaatggttc tgacgctggt
      541 gttgctgccc gcagtggcag gaatgatgga acagggcgat gtgggctttg ccactcttgc
      601 agtcgatatg gggatcacca tcggcaaagt gatcgcattt atcgccatta tgatgctggt
      661 aggtcgccgt ctggtgccgt ggattatggc acgcagcgcg gcaaccggtt ctcgcgagct
      721 gtttaccctg tcggtgctgg cgctggcgtt agggattgcc tttggtgcgg tagagctgtt
      781 tgatgtctcc tttgcactcg gtgcgttctt tgccgggatg gtactgaacg agtctgaact
      841 gagtcaccgt gccgcccacg atacgctgcc attgcgcgac gcgtttgcgg tgctgttttt
      901 tgtctccgtc gggatgttgt ttgatccgtt aattctgatt cagcaaccgc tggcagtgct
      961 ggcgacgctg gcgattattc tgtttggtaa gtcgttagcc gcatttttcc tggtgcgact
     1021 gtttggtcac tcccaacgta cggcattaac catcgccgcc agcctggcgc agattggtga
     1081 gttcgcgttt atcctggcgg gactgggaat ggcattgaat ttactgccgc aggccggaca
     1141 aaacctggta ctggcagggg cgatcctgtc gattatgctc aacccggtac tgttcgcact
     1201 actggagaaa tatctggcga agaccgaaac gctggaagag cagacgctgg aagaggcaat
     1261 cgaagaagag aagcagatcc cagtggatat ttgcaaccat gcgcttctgg tgggttacgg
     1321 tcgtgtaggc agcctgctgg gggagaaatt gctcgcctct gatattccgc tggtggtgat
     1381 tgagacgtca cgaacccgtg ttgatgagct gcgagagcgc ggggtccgcg cagtattggg
     1441 caatgcggcg aacgaagaaa ttatgcaact ggcgcatctg gaatgtgcaa aatggctgat
     1501 cctgacgatt cccaacggtt atgaagcggg cgagattgtg gcatctgccc gcgcgaaaaa
     1561 cccggatatt gagattatcg cccgcgccca ttatgacgat gaagtgacgt atatcaccga
     1621 acgtggcgcg aatcaggtag tgatgggtga acgtgaaatt gcccgtacta tgctggaact
     1681 gctggaaacg ccaccggcgg gtgaagtggt gacggggtaa acggtggttg ccggatgtaa
     1741 aatatgaaaa atatgtaggc atgataagac gcgccagcgt cgcatcgggc atcgagagcc
     1801 aaatgccaga tgcggcgtga acgccttatc tggcctacga accgacgata acttaacgat
     1861 cccagtaaga ctcttccagg ctgtcttcac gctccggcaa gccgcgcgtt aaacgaggtg
     1921 aatgctggtt cagtacctga tagctcacac ggttagcata tttacacacc tgcgctaacg
     1981 atgaataagt taaccaggtg aatttatgtt tgctggagtt tggtacgttg ctacgatggt
     2041 agctgttggc ggtaatgtca tgcagcaacg ctgccaatgc gccatccccc gctccattag
     2101 tgttcatgat tttttccggc ccgcccatgt acggcgcaat gtgcgaatat acacgcagcg
     2161 gattctggca atccttgtgg cgcatggcgc ggctaaactc atactggttg aattccgcta
     2221 tagcgcccgg cagcagcgga tgctgggttt tacgtttcgc ttcgtcttcg gtaaagcccg
     2281 ccatatacaa gccgattggc ccggcggtgc acagcaccag atctacccag tccagcgcct
     2341 tgtcagatgc caacaacgga tcgctttctc cggtcaacgc ttcggcttca tcttcgttca
     2401 tcgcaaggat agagacgtgg tctttgagga attgctgcca ccactgcgga ttctcggcaa
     2461 tgacaaactt agttcccagc gtcagcacca ccggtacgtt atatttcttc gcgtactcaa
     2521 tggctttcat ggttgcttcc ggcatgggtt cacccggctt gcaacgcacc agataagagg
     2581 tgagaaccag agccgaggct ccggcaatca catcttccgg aatactttca gcccgcagct
     2641 ggttcatgtg gccagggctg atagcaaagg tacgttcccc ggactcgcca atcagcgtaa
     2701 agcaacgacc aatcgggcca tccacgcctt gtagatagtt aagatcggta cggctggagg
     2761 tgttacacag gtaacgatag gcatagctgc caatttcaat attgctgcac atgacgccca
     2821 gcagcaccga acggtcgtcc gcgagcaccg agtagttatg catggtgtta ccaatggtgc
     2881 cacccgcaaa ctgatgggta atcaggtttt tctgttttag ttcctgataa agcgcttcgg
     2941 ctacatcatc ctcaatcacc agtgaatgcc cggcgcttaa tccataacgc tcaatgaatt
     3001 catcatccac tttcgcttca atatcgacca gcgtttgatc gatacccact acccaggcag
     3061 cgctggtttc gttttctggc tggaattgct gaagcagcgg atcgcgtgca tttacgggga
     3121 agtaatgttt ggatttacgt ttaccgggaa atttcatggt tgtttttacg ggtggttaac
     3181 tgagcggcga atgttaacac aatctgacgg gagcgcgcga gacgcagcca gtttcaatat
     3241 cagcaggcgg gatcgcattt tcatggcagt ggttccttac aatgacgtaa tttgaaagga
     3301 gtttttgtat gaagccggaa aacaaactac ctgttctgga ccttatttct gctgaaatga
     3361 agaccgttgt gaatactctt cagtcggatt taccgtcctg gcccgcaacg ggaacgattg
     3421 ctgagcaacg acagtattac acgcttgagc gccgattctg gaatgcgggc gctccagaaa
     3481 tggcaaccag agcttacatg gttccaacaa aatatgggca ggtggaaaca cgtctctttt
     3541 gtccgcagcc agatagcccg gcgacgctat tttatttgca tggaggcggt tttattctcg
     3601 gcaatctcga tacccacgat cgcatcatgc gcctgctggc aagctacagc caatgtacgg
     3661 tgattggtat tgattacccc ctttcacctg aagcgcgttt tccgcaagcg atagaggaaa
     3721 ttgtggctgc ttgttgttat ttccaccagc aggcggagga ttatcaaatc aatatgtccc
     3781 gcattggctt tgccggtgat tccgcaggtg ccatgctggc gctcgccagt gcgttgtggt
     3841 tgcgtgataa acagatcgat tgcggtaaaa ttgcgggtgt tttgctgtgg tatgggcttt
     3901 acggattacg ggattccgtg actcgtcgtc tgttgggcgg tgtctgggat ggcttaacgc
     3961 aacaggattt gcagatgtat gaagaggcgt atttaagcaa cgacgcggac cgcgagtcac
     4021 cgtattactg tctgtttaat aatgatctca ctcgcgaagt tccgccctgt tttattgccg
     4081 gggcggagtt cgatccgctg ctggatgaca gccgtctgct ttaccagacg ttagcggcgc
     4141 atcagcagcc ctgtgagttc aaactctacc caggtacgct gcatgccttt ttgcattatt
     4201 cacgaatgat gaaaaccgcc gacgaggctc ttcgcgacgg cgctcagttc tttaccgctc
     4261 agctttagcg atacgcggca acaagattag ccatcatttc gatatgttcc ggcgtggcat
     4321 taagcgctgg aatatattca tattttttcc cgccggcacc gaggaagacc tcacggtttt
     4381 gctcggcaat ctcttccagc gtctccagac aatccgcagc aaagcccggg cacatcacct
     4441 gtatatgacc tacgcctttt tctccgagca ttttcagcgt ttcgtcggta taaggcatca
     4501 gccagggttc ccggccaaag cgcgactgaa aggtcatcat cactttttcc ggtgccatcc
     4561 ccagtgcgga agccagttcg cgagtcgttg tgcggcaacg ttgcgggtaa tcatcgcctt
     4621 catctgcata acgctgggga atgccatgat aagagagcag cagcagatcc ggttcgccat
     4681 gtttggcaaa agaagcgcgt acgctgttcg ccagtgcatt aatgtaatcg tggttatcgg
     4741 cgtaatcacg aataaacgat atccccggaa tgctacgttt gcgcgccaga atgcgtgcca
     4801 gttcatccca taccgcaccg accgtagaac aggagaattg cggataaagc ggcagcacca
     4861 caatatgatc tacatgctct gccaggagtt catcgacggc gctttccagt gatggcgagc
     4921 cgtagctcat gcccagcgcc actggcgtat ccggtaaacg ttgtgccaac gcctgctgtt
     4981 gctggcggct gtaaaccatc agcggcgagc cgtcttccat ccagaccgag gcgtacagct
     5041 tcgccacacg cggcgagcgc agcggcaaaa tcacgccgcg cagcaacggc caccataaca
     5101 accgtgaggt atcaaccacg cgtctgtcgc ttaaaaattg tttcagatag cgttttaccg
     5161 cttcaggtgt gggggcatcg ggcgtaccca ggtttgccag caggataccg gttttagtct
     5221 gacgcattac cgcctcttat cgattcaact tgttgataat tgtagcggaa aagtgaggaa
     5281 gaagaactaa ttgctgtgag aggtcaaatc agaaaaagga atagcgcaga ttgaggtttt
     5341 tgtaaaaggc cgaatgaata tccggcctga gattgctgat aagtttgctt gtgcgggcct
     5401 gttcccctca ccctaaccct ctccccgagg gggcgagggg actgtccgtg cgcgctttcg
     5461 aattagccga ggattttttc cagatcagcg cgaacttcag caaccggctt ggtgccgtca
     5521 actttcgcgt atttggtgtt acccgcttcc gcttctttgg agtagtagcc gatcagcggt
     5581 gcagtcatct gatggtattc aaccagacgt ttacgcacgg tttcttcctg atcgtcttta
     5641 cgggtagtca gttcttcacc ggtaacgtcg tctttgcctt ctactttcgg cggattgaat
     5701 ttaacgtgat aaacacgacc agacggcgca tgaacgcggc ggcctacgat acggtcaacg
     5761 atcagttcgt ccggtacgtc gaattccaga acgtaatcaa cattgatgcc cgcttctttc
     5821 atcgcgtctg cctgcggaat ggtacgcggg aagccgtcca acaggaaacc attacggcag
     5881 tcttcctgag caatgcgctc tttaaccagc gcgatcacca gttcgtcggt gaccagtttg
     5941 ccagcatcca taatgtcttt tgcttgttta cccagctcgg agccagattt gaccgcagca
     6001 cgcagcatat cgccagtgga gatttgcgga ataccatatt tctccatgat gaactgagcc
     6061 tgagtccctt tccccgcgcc cggagcgcca agcagaatga tacgcattgc gaaaatcccc
     6121 ttaaaatgtg tcgaattttt tgaaaaagcg ataaacgata ccaccaacag gcgattgcct
     6181 caagaaaggc acctgaggct gaaacggtta atggcgagat aatttgcgga aagtgcagat
     6241 gatgaaaaga aaaatgccgg atgacacgaa ggtcatccgg catgacatca ggaaaccagc
     6301 agctggttca tacgacgaat aaacagattc ggatcttcca gcgtgccgcg ttctgccagc
     6361 agcgcctgat ccagcagcag ttctacccac tcgctgaact tcgcttcatc ttcagtatct
     6421 gccgcacgtt tcaccagtac gtgatccggg ttcagttcga agatgtattt cacttctggc
     6481 actttctggc ccgccgcagc gaacagtttc gccatctggg tgctcatttc atccgcgtcg
     6541 gtcgaaacga tcgctggcgt atcggtcaga cggtgagtca gacggacatc tttcacgcgc
     6601 tcgccgagca gggctttcac acggtcgatg aacggagtca gtgctttctc cgcttctttc
     6661 gcgctctcat caacttcgtc agccagtttt tccagcgact cgtcaacttt agacacagac
     6721 tggaacggtt taccgtcaaa ctcagtcaga tagttcatca tccactcatc gatgcggtcg
     6781 gaaagcagca gaacttcgat gcctttctta cgcagcagtt ccaggtgcgg actgctcttc
     6841 gctgccgcat agctgtctgc agtgatgtag tagattttct cctgcccttc tttcatgcgg
     6901 gaaacgtagt cttccagaga cacggtctgc gcagaagaat cggtatgggt agaagcaaaa
     6961 cgcaacagtt tggcgatcgc ttcctggtta gcgaaatctt ccgccggacc ttctttcagt
     7021 accaggccaa actgttgcca gaaggtctgg tatttttccg cgtcgtcttt cgccagtttt
     7081 tccagcattt gcagcacacg cttggtcagc gcattgcgca ggttacgcgt taccgtgctg
     7141 tcctggagga tttcacggga aacgttcagc ggcagatcgc tggagtcaat cagaccacgc
     7201 acgaagcgca gatagttcgg catgaactgt tctgcgtcgt ccatgatgaa cactcgctgg
     7261 acgtacagtt tcaggccgtg tttatgatcg cggttccaca tatcccacgg agcctgggac
     7321 ggaatgtaca gcaggctggt gtactcctgc ttaccttcaa cgcggttgtg gctccaggtc
     7381 agcggatcgt taaagtcgtg ggcgatgtgt ttgtagaact ctttgtactc ttcatcggtg
     7441 atttccgact tgttacgagt ccacagcgcc tgcgctttgt tgattttctc ccaggagata
     7501 acggtttcgc cgtctttctc ttcgcgtttt tcgatctcta ccggcagcgc gatatgatcg
     7561 gagtatttgc tgatgatgga acgcacgcgc cagtcatcga ggaactcgtc ttcgccttca
     7621 cgcagatgca gggtgatttc agtaccacga tcttctttgg tgatgtcggc aacggtgtat
     7681 tcaccttcac cagccgattc ccagaacacg ccattttctg gtttttcgcc tgccgcgcga
     7741 gtacgcacgg tcactttgtc ggccacgata aacgcagagt agaaaccaac accgaactga
     7801 ccgatcagct ggctgtcttt cgcctggtca gaacccaggg attcgaggaa tgatttggtg
     7861 ccggatttag cgatagtccc cagatggtcg atcacttcat cgcgggtcat ccccacgccg
     7921 ttatctgaga tggtcagcgt gcgcttgtct ttatcgaaag agacacgaac gcgcagttcg
     7981 ccatcacctt cgtacaggtc cgggttagag agcgcgcgga aacgcagctt gtccgccgca
     8041 tcggaggcgt tagagataag ctcacgcagg aagatttctt tattggaata gagagaatgg
     8101 atcatcaggt gcagaagctg tttcacttct gactgaaaac cacgagtttc ttgtcctttc
     8161 atgtaggtct acctcaataa tgccatttta aggttaaaaa caggatgtgg gagagatggg
     8221 gacaagggag aataatttca agcgagagca ggtgatcctg ctctcgtttg cttaaaaacg
     8281 aatcttatga cgcccggcaa gggagtgtga cagcgtggtg ccatcgacca tttccagttc
     8341 gccgccaacc ggtacgccat gagcgattcg gctggcttcc acgtcatatt gcgcgcaaag
     8401 ctcggcaatg tagttagcgg tagcttcacc ttcaaccgtg ggattggtgg cgaggatcac
     8461 ttcagtgatt ttttcctctg ccagacgctg ttccagacga tcaagcccga tatcatccgg
     8521 accgatgccg tccagcggtg acagatgccc catcaacaca aaataacgac cagaaaactg
     8581 ccccgtctgc tcaatggcgt agatgtccgc cggactctcc accacacaga tttgaccgtt
     8641 ttcctgacga cgcggattcg aacagatgtt acagacttcc tgttcggtga aagtgcggca
     8701 atcggcgcag tggccgattt ccgacatcgc ccgggtgagc gcctgcgcca gacgcatccc
     8761 gccgctacga tcgcgctgaa gcagcgtgaa cgccatacgc tgcgccgact tcgggccaac
     8821 gcccggcaga cagcgcagtg cttccataag ctgtgttaac agcgggctgg tttgcatcag
     8881 aacggcatct taaagccagg cggcagctgc attccggagg atacagaagc cattttttct
     8941 ttctgcgttt cttcgatacg acgtgctgcg tcgttgaatg ctgcagccac caggtcttcc
     9001 agcatctctt tgtcgtcttc cagcaggctc gggtcgatct ctacgcgacg gcagttatgt
     9061 gcaccgttga tggtcacttt taccagacct gcgccagatt cgccggtaac ttccagctgc
     9121 gcgatctctt cctgcatttt ctgcattttt tcttgcatct gctgggcttg cttcatcagg
     9181 ttacccagac cgcctttacc aaacataggt ttctctctca atcacgttaa ggatgacgaa
     9241 cgtaagctgt gcttacgatc aaatggggcg gatactttct tcatccagct ccgcatcgaa
     9301 gaatcgacgc agggtctgaa tattattatc cgcaataatg gactcgcgcg cctgcgcaag
     9361 tttttcttcg tatatcgcct gacgccactc cagcggcgta cgcaccgcgg gattatcatc
     9421 ttcaacgata gtcagttcaa ccgttgaacc ttttaacatg ctcaacgctt cagccagttt
     9481 ttgctgtgca ccgcggttgt tcaaatgccg ctgagaggag cgcaaatgca gacatactgc
     9541 gttatcactc tcctctttcc aggcatttaa ggccacctgt tcgaccagtt ttggtagcga
     9601 aagttgactc acctgagccg cccacgggtc gcgctcaatg gcttctgccg ccagcttcgc
     9661 cgccagttcc ggcgtttttt catgttccag cgcttttttc agcgccttcg gcgtggcgac
     9721 cacttctttt tgctgcatca ctggagtggt cgccttccag cgatacgctt cttttttagc
     9781 tggcgctttt tccagcgccg atggcaccgg acgcgcctga acgcgatctg tgaccgaagc
     9841 cagtctttcc agcgcagcgt tattcaccgg ccgcgcgcgg gtagcggctg ccggttcact
     9901 cttttttgct ttggttgctc cctgcacgcg ctgcaactgc tggcgcgccg ccagcacctg
     9961 gctggtggtt tccgggagcg gtacagtcgg agcctgctgc ggcgctgatt gcggttgcgg
    10021 cggcacctgg gttggcgtca ttactgccgt tggcgcgacg ggtgcaaagg actggcgtgg
    10081 cacttcaggc tcaggcagcg gcatacgcgg atggaacgcc agcgcgcgca gcagcgtcat
    10141 ctcaacgccc atgcggcggt ccggcgcata cggtaattct ttgcgaccaa tcaacaacgt
    10201 ctgatagtaa agctggatat ccgtcggcgg tatggtgcgc gccagttcac gcatccgcag
    10261 ctcgatggcg gccatatcgt tgccaagtgc agcaggcgaa agttgtacca tcgcaatacg
    10321 atgcaacagg ccaagcattt ccaccagcaa cgcttcccac tcgataccac gggcagcggc
    10381 ttcattaatt agcgccatta cacgctcgcc gttggcctcg accatcgctt caaccagcga
    10441 cagcgcctga tcgtcgtcaa gcgtacccag catcgcactg accgcctggg ttgaaacctg
    10501 gccgtcaccg ctggcaatcg cctggtcggt cagacttaag gcatctcgca agctgccttc
    10561 agcggcgcgc gccagcaatt gcagcgcccg cggctcgtgg gcgatatgtt cttcgttgag
    10621 gatgtgctca agctgatggc gaatttgctc gacatccagc gccttgagat gaaattgcaa
    10681 acagcgcgac aaaatcgtca ccggcaattt ctgtggatcg gtcgtcgcca gcagaaactt
    10741 aacgtgctcc ggcggctctt caagggtttt taacagtgcg ttaaagctgt ggcgcgacag
    10801 catatgcact tcgtcgatca gataaacttt gaaacgacca cgcgccggag cgtactggac
    10861 gttatccagc aggtcgcggg tatcttcaac tttggtgcgc gaggcggcgt cgatttcaat
    10921 cagatcgaca aagcgcccct gctcgatttc acgacagtta tcgcacacgc cgcacggcgt
    10981 cgcggtaatg ccggtttcgc agtttagccc cttcgccagc agtcgggcga tagaggtttt
    11041 tccgacgcca cgggtaccgg aaaaaagata agcatgatga atacgcccta acgacaagcc
    11101 gttcgccagt gcggtcagca catgttcctg gccgacgacg tcagcaaagg tttgtgggcg
    11161 ccattttcgg gctaagacct gataactcat tggcaggctc tgaaacgctg gaaggtggat
    11221 tcacgaaggg gtaatgctaa cacagccccg tcagaacggc gaggctgaat gcacatcttg
    11281 tgctgtccgt agcgtgggca gcacaagact ggcgataatt aatggcccgg gaacgggaca
    11341 aggctgtagc tggtaatgcc ctgtttttcg agacgctgtt cgccgccgag atcgaacagg
    11401 ttgataatga acgcagcgtc agccacttca ccacccagac gacggatcag tttaacggtc
    11461 gcttcgatag tgccgccggt tgccagcaga tcgtccacca ccagaacttt gtcgcccggt
    11521 ttgatggcat caacgtggat ctccagctga tcggtgccgt attccaggtc gtaagtttca
    11581 ctgatggttt cacgcggcag tttgcccggt ttacggaccg gtacaaagcc aacgcccaga
    11641 cccagagcta ccggagcgcc aaacaagaag ccacgcgctt cggtgccgac aactttggta
    11701 atgcccgcat ttttgtaacg ctcaaccagc aagtcgatgc tgagagcgta agctttcggg
    11761 tcttccagta agctggtgac atcgcggaaa agaatgccgg gttttgggta gtcctgaatg
    11821 cttttgatgc tatttttgag atactcaagc tgctgtgcag tcgcggtcat aagtgtatgc
    11881 ctgcttgtta cggtggtact cacggcgcgt tttaaacgta tcaaaagtaa cagttgttta
    11941 acctttgacc gcctgcgcct gtgctcgaaa acgccagaat gtactggctg tacgcaataa
    12001 ttgcaactgc gattgttgtg cttcagtgct tttcttgctt ttcatcaatc accggaattc
    12061 gccacatata gaaaagcaaa caggcgagaa ttaccagcaa catgatgcgc acccatggca
    12121 tctggacgaa ccacagagaa atggcaaacg tgagcaaaat aagcaaaatc gcccgcggtt
    12181 tgacgccgcg cggcatcgca tgatgtttct gccagaaacg tagatagctg ccaaaccatg
    12241 agcggtacag caaccaggcg tgaaagcgcg gggaagaacg ggcaaagcac caggccgcca
    12301 gcaggataaa cggcgtcgtc ggtaataccg gtaataccac gcccagcgta cccagcacta
    12361 ccgccagcca gccaatgatg attaaaatga ttcgttgcat attgagtgtt gtcagcttac
    12421 agagtggcta ctttagcata acaattatca ttttcattga ggtcttatcg tgaaaaccgc
    12481 cctgctgctg gaaaaactgg aaggacagct cgctacgctg cgtcagcgtt gtgccccggt
    12541 gtcacagttc gccacgctaa gtgctcgttt cgacaggcat ctttttcaga ctcgtgcgac
    12601 aacactacag gcttgtctcg acgaggcggg cgataatctg gctgcgcttc gtcatgcagt
    12661 tgagcagcaa cagctgccgc aagtggcctg gctggcggaa catctggcgg cacaactgga
    12721 agccatcgcg cgtgaagcct ccgcctggtc attgcgcgag tgggacagtg caccaccgaa
    12781 aattgcccgc tggcagcgta aacgtattca gcatcaggat tttgagcggc ggctacgtga
    12841 gatggttgcc gaacgcagag cccgtctggc gcgggtgacc gatctcgtgg aacagcaaac
    12901 gctgcatcgt gaagtggaag cctatgaagc gcgcctggca cgctgccgcc atgcgctgga
    12961 aaaaatcgaa aacaggttag cgcgtttaac ccgctagcaa tggagagaat atgtcactgg
    13021 aaaatgcccc tgacgatgtc aaactggctg tcgatttgat tgtgctgctg gaagaaaatc
    13081 agatcccagc ccgcaccgtg ctgcgcgcgc tggatattgt aaaacgcgat tatgaaaaga
    13141 aattaacgcg cgatgatgag gcggaaaagt gagaaataaa tggggccgct aaatgtcccc
    13201 taagattgtg ggcaaacgca aaactgcctg atgcgctacg cttatcaggc ctacaccgct
    13261 cctgcaatac attgaattct tgcggtttcg taggccggat aaggcggtca cgccgcatcc
    13321 ggcatgaaca acgcgcactt tgtcagtaat ctcggggcaa ctaaatgtcg ccacctttgg
    13381 ttattaccct accgctggcg tcgggtcatc gcctttgtag tcgcgtttga cttccgtcac
    13441 ctcatcgccc ttctcgttgt gcagatgcac ttcaagctgg ttaaaggcaa tgttgatgtc
    13501 gttttcacgg cacagctgat cgatagtacg gttcagctca tcgacagtac gactacggtc
    13561 acgcagttca cgcacataca gacgcagctc atgatccaac gtgctggcac caaacgccgt
    13621 aaagaagact tccggcattg gttcgtgcat cacccttggg tgctcagtcg ccgccttcag
    13681 taacacttta cgcacttttt ccagatcgga gccataggcc acgccgagac ggatcaccag
    13741 acgcgtagta gtgtcagtca acgaccagtt gatcagacgc tcggtaacaa acgctttgtt
    13801 cgggatgatc acttctttgc gatcgaaatc ggtaatcgtt gtcgcacgaa tacggatctt
    13861 acttaccgtc cccgagaagc taccaatggt taccgtatcg ccaatacgca ccggacgttc
    13921 gaacagaatg atcaaaccgg agacgaagtt accgaaaatt tcttgtaaac caaaaccaag
    13981 acctacggat aatgctgcgg ccagccactg gagtttatcc caagagacgc ccagcgatcc
    14041 gaacaccgtc atcgcaccaa cggcaataat gatgtagtta aggatggtag taatggcata
    14101 cgacgcgccc tgacgcatat tcagtcgcga gagcaccagc acttccagta aaccaggcag
    14161 gttgcgaatc aacgcccagg ccaccattga ggcgataatc gcaaacaaca gactgcccat
    14221 ggtgacgttt ttcaccaccg cagcgccagc ttcagtgccg ttgtaatgcc agagcgtgat
    14281 gctgtcgaga tagctgaaca cggtgatcaa atcggaccaa attgcccaga acatgacacc
    14341 gaacagcgca aacatcagca acatggtaat acgcagcgtc tgctggttaa cttgctccag
    14401 tgcaatggtg ggttcttccg gcggttcggc accttctgcg ccctctttca ccagattctg
    14461 ccgacgcgcc agcgcacgac gccaggcgat acgccgcgcc gctacgctta agccgcgcaa
    14521 taccgtctgg tacagcaggt tccagatgat caccaaataa acggtttcaa tccaacgccc
    14581 ggagaggcgc agcgtggtgt agaaataacc cgtggcagtc agcaccatca acgcaatcgg
    14641 gataatcgat agcacggtaa tggtgaccag tcgcatggtg tgcgactctt tatcacgcca
    14701 gctttcgcgg cacattggcc ataccaggaa ggcaatcagc agcaggttga agaaaatcat
    14761 cgcctgcccc agcacatcat ccatcagatg cagcggggaa agttctgcta ccacagacca
    14821 gaaatggata ggcagcaacg cgagactgat gcggacaatt tgccgacgcc agtggctggt
    14881 ctgctgctcc ggcatgccga agtgacgcac ggcaacgcca tttttctcca gtaccttcca
    14941 gcacaggcca aacaccagcc agaaaatcgc cagtttttta ctgaacgacc atagcagttc
    15001 gctgatgttg agctgcatgg tcaacagaat caggccaacc gcgagaataa tcaggcacac
    15061 cggcagcgcg cggatcaggt cgataaggat cgcttttggt gtgttgagct ggctatcgtt
    15121 acgcagggaa cccacagcgg aagccagttt ctgttgatac gctttcagcc agcccagacg
    15181 ccagtggatc agcccggcaa tcaacagcag cggcaaaccg gcgaggaaag cgataaaaac
    15241 ggcaggccag gctttttccc agttcaccgt gattttcatc gacttaaatt catctttcag
    15301 gctttgcggg aacgctttga tccagtccca gtccattgga cggttactgt tcacccaaaa
    15361 gatttgctga gtcaggatgg atttcaggtt tttcgacaca ctcattaact gctgctggtt
    15421 gatttgcagg ttaatggcca tcatcagctg gttacccaac tgtttgttga gttgatccag
    15481 caattcgcga cgcatatcaa ccacttgcaa taacgcatcg tgaacttcgc tgttgacttc
    15541 gttggtgtga ccttcttcca gtttgttgac gaacgcatcg ctctggaaaa gtgcatcacg
    15601 ctgctggtta acttcaaact gttcgagacg caaatccgcg atgcggttgg tcatgttttc
    15661 cagttcatcc gccgagggca gcgtttgttg ttgctggtaa aggatacgag acaacagcag
    15721 gctgcccttc aggacggcaa tctgctcttt aatattgcgt tccgattgca gcgcccgctc
    15781 cagccagttt ttgactttaa tgttttgctg catcaactga ttaccgtttt cagtcgcggt
    15841 aatcagacgc tgacttaact gctggttaat ttccagttcc tgtttcacca gcggattagc
    15901 ctgaatacgc gcggcttcat ccggggagac ggcttcctgc gccgtttttt cagttaaagt
    15961 caggcgcttg ctgtttaccg cttcttgcaa cagttgtaac tggtgctcca gacgagcgct
    16021 gttcgccgtc acgtaatcac gttgcttttg caaggtatcc tgcaagacgg tgttcccttc
    16081 caggctttta cgctgctggt caatctccgc attcagcaac gcctgctggg cctgcattaa
    16141 cactttctgg ctgggacgta aggctgtctc gccgacatca gtcccatcca gacgactgcg
    16201 aatttgttgc agctgctgcg aagcgttata catcgcattt tgcacgcgtt cgggctgcgt
    16261 ctgtaacgaa accagctggc tgttataaga cgccagatcg ttttgtgcgt tttgcaaatc
    16321 gtccagcgcc tgggcaacgc gagtttccag ctggcgcaac gaaagcgtgc tcagaatttt
    16381 gcgcgtttct tcgtcgttat cgacatcgct aagtgctgtt aacgccgcgg tcgcctggcg
    16441 cattttttcc ggcgcttcag cgactttttg ccgtagctga actgtctctt cttttatgcg
    16501 atcgatttta tcgagggtgg ctaatgtatc tgtcagatcc tgctgcacca gtttgtcctg
    16561 agcagaaaga tctttttgtt tatttagtga gtcaagttgc gcctgcaggt ccgcttttgt
    16621 cggcagatca ccattcgatg acgcccgcgc aaacgccgtg ttctggcata acaagacaaa
    16681 aacaaaagca ataaatgctg aaaaaacaaa atgccgtgat cgtttgtaat actggaacat
    16741 agtcatgatg aatgaaggtt tctgaacctg aagaacgacc tgaaaaagtc aaaccgcaag
    16801 aatatcacga cgcagtgaac cagaatagca acgacgaaaa tgtccaggaa aaatcctgga
    16861 gtcagattca gggttattcg ttagtggcag gattacgaag cgtggggcac aggagataca
    16921 tctccagtaa gatggcgacg taatcgcggg cttctttttt aagatcaaaa gattgcgggg
    16981 caaagagcca gttttccatc aggccggaaa tatagccgcg cataataatt gctgcccgac
    17041 gcgtcattaa atccgcaggc aacattttcg cttcaataca atgttttaac gtttgttcta
    17101 tacggtcata actttccaga cagagattac gttgtgcctg ttgcacaaca gccatttctc
    17161 cgacaaattc gcatttgtgg aatataatct ccatcaataa tcgacgtcgt tcttctgtca
    17221 ccgtggattc aagaacatga attaatatct ctcttaatac tgagagtgga tcgccaggga
    17281 attttgcctg atactcaagc tctagttcac caatattgga ttctgacagt tcccagatct
    17341 cactgaacaa atccgacttg tctttaaaat gccagtagat tgcaccgcgc gtaacgccag
    17401 ctgcttttgc aatctcgccc agcgaggtgg atgatacccc ctgctgtgag aaaagacgta
    17461 gagccacatc gaggatgtgt tggcgcgttt cttgcgcttc ttgtttggtt tttcgtgcca
    17521 tatgttcgtg aatttacagg cgttagattt acatacattt gtgaatgtat gtaccatagc
    17581 acgacgataa tataaacgca gcaatgggtt tattaacttt tgaccattga ccaatttgaa
    17641 atcggacact cgaggtttac atatgaacaa aaacagaggg tttacgcctc tggcggtcgt
    17701 tctgatgctc tcaggcagct tagccctaac aggatgtgac gacaaacagg cccaacaagg
    17761 tggccagcag atgcccgccg ttggcgtagt aacagtcaaa actgaacctc tgcagatcac
    17821 aaccgagctt ccgggtcgca ccagtgccta ccggatcgca gaagttcgtc ctcaagttag
    17881 cgggattatc ctgaagcgta atttcaaaga aggtagcgac atcgaagcag gtgtctctct
    17941 ctatcagatt gatcctgcga cctatcaggc gacatacgac agtgcgaaag gtgatctggc
    18001 gaaagcccag gctgcagcca atatcgcgca attgacggtg aatcgttatc agaaactgct
    18061 cggtactcag tacatcagta agcaagagta cgatcaggct ctggctgatg cgcaacaggc
    18121 gaatgctgcg gtaactgcgg cgaaagctgc cgttgaaact gcgcggatca atctggctta
    18181 caccaaagtc acctctccga ttagcggtcg cattggtaag tcgaacgtga cggaaggcgc
    18241 attggtacag aacggtcagg cgactgcgct ggcaaccgtg cagcaacttg atccgatcta
    18301 cgttgatgtg acccagtcca gcaacgactt cctgcgcctg aaacaggaac tggcgaatgg
    18361 cacgctgaaa caagagaacg gcaaagccaa agtgtcgctg atcaccagtg acggcattaa
    18421 gttcccgcag gacggtacgc tggaattctc tgacgttacc gttgatcaga ccactgggtc
    18481 tatcacccta cgcgctatct tcccgaaccc ggatcacact ctgctgccgg gtatgttcgt
    18541 gcgcgcacgt ctggaagaag ggcttaatcc aaacgctatt ttagtcccgc aacagggcgt
    18601 aacccgtacg ccgcgtggtg atgccaccgt actggtagtt ggcgcggatg acaaagtgga
    18661 aacccgtccg atcgttgcaa gccaggctat tggcgataag tggctggtga cagaaggtct
    18721 gaaagcaggc gatcgcgtag taataagtgg gctgcagaaa gtgcgtcctg gtgtccaggt
    18781 aaaagcacaa gaagttaccg ctgataataa ccagcaagcc gcaagcggtg ctcagcctga
    18841 acagtccaag tcttaactta aacaggagcc gttaagacat gcctaatttc tttatcgatc
    18901 gcccgatttt tgcgtgggtg atcgccatta tcatcatgtt ggcagggggg ctggcgatcc
    18961 tcaaactgcc ggtggcgcaa tatcctacga ttgcaccgcc ggcagtaacg atctccgcct
    19021 cctaccccgg cgctgatgcg aaaacagtgc aggacacggt gacacaggtt atcgaacaga
    19081 atatgaacgg tatcgataac ctgatgtaca tgtcctctaa cagtgactcc acgggtaccg
    19141 tgcagatcac cctgaccttt gagtctggta ctgatgcgga tatcgcgcag gttcaggtgc
    19201 agaacaaact gcagctggcg atgccgttgc tgccgcaaga agttcagcag caaggggtga
    19261 gcgttgagaa atcatccagc agcttcctga tggttgtcgg cgttatcaac accgatggca
    19321 ccatgacgca ggaggatatc tctgactacg tagcggcgaa tatgaaagat gccatcagcc
    19381 gtacgtcggg cgtgggtgat gttcagttgt tcggttcaca gtacgcgatg cgtatctgga
    19441 tgaacccgaa tgagctgaac aaattccagc taacgccggt tgatgtcatt accgccatca
    19501 aagcgcagaa cgcccaggtt gcggcgggtc agctcggtgg tacgccgccg gtgaaaggcc
    19561 aacagcttaa cgcctctatt attgctcaga cgcgtctgac ctctactgaa gagttcggca
    19621 aaatcctgct gaaagtgaat caggatggtt cccgcgtact gctgcgtgat gtggcgaaga
    19681 ttgagctggg tggtgagaac tacgacatca tcgcagagtt taacggccaa ccggcttccg
    19741 gtctggggat caagctggcg accggtgcaa acgcgctgga taccgctgcg gcaatccgtg
    19801 ctgaactggc gaagatggaa ccgttcttcc cgtcgggtct gaaaattgtt tacccgtacg
    19861 acaccacgcc gttcgtgaaa atctctattc acgaagtggt taaaacgctg gtcgaagcga
    19921 tcatcctcgt gttcctggtt atgtatctgt tcctgcagaa cttccgcgcg acgttgattc
    19981 cgaccattgc cgtaccggtg gtattgctcg ggacctttgc cgtccttgcc gcctttggct
    20041 tctcgataaa cacgctaaca atgttcggga tggtgctcgc catcggcttg ttggtggatg
    20101 acgctatcgt tgtggtagaa aacgtggagc gtgttatggc ggaagaaggt ttgccgccaa
    20161 aagaagctac ccgtaagtcg atggggcaga ttcagggcgc tctggtcggt atcgcgatgg
    20221 tactgtcagc ggtattcgta ccgatggcct tctttggcgg ttctactggt gctatctatc
    20281 gtcagttctc tattaccatt gtttcagcaa tggcgctgtc ggtactggta gcgttgatcc
    20341 tgactccggc tctttgtgcc accatgctga aaccgattgc caaaggcgat cacggggaag
    20401 gtaaaaaagg cttcttcggc tggtttaacc gcatgttcga gaagagcacg caccactaca
    20461 ccgacagcgt aggcggtatt ctgcgcagta cggggcgtta cctggtgctg tatctgatca
    20521 tcgtggtcgg catggcctat ctgttcgtgc gtctgccaag ctccttcttg ccagatgagg
    20581 accagggcgt atttatgacc atggttcagc tgccagcagg tgcaacgcag gaacgtacgc
    20641 agaaagtgct caatgaggta acgcattact atctgaccaa agaaaagaac aacgttgagt
    20701 cggtgttcgc cgttaacggc ttcggctttg cgggacgtgg tcagaatacc ggtattgcgt
    20761 tcgtttcctt gaaggactgg gccgatcgtc cgggcgaaga aaacaaagtt gaagcgatta
    20821 ccatgcgtgc aacacgcgct ttctcgcaaa tcaaagatgc gatggttttc gcctttaacc
    20881 tgcccgcaat cgtggaactg ggtactgcaa ccggctttga ctttgagctg attgaccagg
    20941 ctggccttgg tcacgaaaaa ctgactcagg cgcgtaacca gttgcttgca gaagcagcga
    21001 agcaccctga tatgttgacc agcgtacgtc caaacggtct ggaagatacc ccgcagttta
    21061 agattgatat cgaccaggaa aaagcgcagg cgctgggtgt ttctatcaat gacattaaca
    21121 ccactctggg cgctgcatgg ggcggtagct atgtgaacga ctttatcgac cgcggtcgtg
    21181 tgaagaaagt ttacgtcatg tcagaagcga aataccgtat gctgccggat gatatcggcg
    21241 actggtatgt tcgtgctgct gatggtcaga tggtgccgtt ctcggcattc tcctcttctc
    21301 gttgggagta cggttcgccg cgtctggaac gttacaatgg cctgccatcc atggaaatct
    21361 taggccaggc ggcaccgggt aaaagtaccg gtgaagcaat ggagctaatg gaacaactgg
    21421 cgagcaaact gcctaccggt gttggctatg actggacggg gatgtcctat caggaacgcc
    21481 tctccggcaa ccaggcacct tcactgtacg cgatttcgtt gattgtcgta ttcctgtgtc
    21541 tggcggcgct gtacgagagc tggtcgattc cgttctccgt tatgctggtc gttccgctgg
    21601 gggttatcgg tgcgttgctg gctgccacct tccgtggcct gaccaatgac gtttacttcc
    21661 aggtaggcct gctcacaacc attgggttgt cggcgaagaa cgcgatactt atcgtcgaat
    21721 tcgccaaaga cttgatggat aaagaaggta aaggtctgat tgaagcgacg cttgatgcag
    21781 tgcggatgcg tttacgtccg atcctgatga cctcgctggc gtttatcctc ggcgttatgc
    21841 cgctggttat cagtactggt gctggttccg gcgcgcagaa cgcagtaggt accggtgtaa
    21901 tgggcgggat ggtgaccgca acggtactgg caatcttctt cgttccggta ttctttgtgg
    21961 tggttcgccg ccgctttagc cgcaagaatg aagatatcga gcacagccat actgtcgatc
    22021 atcattgata caacgtgtaa tcactaaggc cgcgcaagcg gcctttttta tgcataacct
    22081 acgaacatta aggagtaatt gaaccaccaa c
//
LOCUS       DAMZII010000078        22071 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZII010000078 DAMZII010000000
VERSION     DAMZII010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.078"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            157..840
                     /locus_tag="POA77_002903"
     CDS             157..840
                     /locus_tag="POA77_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI6366302.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLEYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            884..5734
                     /gene="ehaG"
                     /locus_tag="POA77_002904"
     CDS             884..5734
                     /gene="ehaG"
                     /locus_tag="POA77_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDI6366303.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGNNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTANKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6103..7758
                     /gene="lldP"
                     /locus_tag="POA77_002905"
     CDS             6103..7758
                     /gene="lldP"
                     /locus_tag="POA77_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6366304.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7758..8534
                     /gene="lldR"
                     /locus_tag="POA77_002906"
     CDS             7758..8534
                     /gene="lldR"
                     /locus_tag="POA77_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDI6366305.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8531..9721
                     /gene="lldD"
                     /locus_tag="POA77_002907"
     CDS             8531..9721
                     /gene="lldD"
                     /locus_tag="POA77_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDI6366306.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9875..10348
                     /gene="trmL"
                     /locus_tag="POA77_002908"
     CDS             9875..10348
                     /gene="trmL"
                     /locus_tag="POA77_002908"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI6366307.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10401..11222)
                     /gene="cysE"
                     /locus_tag="POA77_002909"
     CDS             complement(10401..11222)
                     /gene="cysE"
                     /locus_tag="POA77_002909"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI6366308.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11302..12321)
                     /gene="gpsA"
                     /locus_tag="POA77_002910"
     CDS             complement(11302..12321)
                     /gene="gpsA"
                     /locus_tag="POA77_002910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI6366309.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12321..12788)
                     /gene="secB"
                     /locus_tag="POA77_002911"
     CDS             complement(12321..12788)
                     /gene="secB"
                     /locus_tag="POA77_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDI6366310.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12851..13102)
                     /gene="grxC"
                     /locus_tag="POA77_002912"
     CDS             complement(12851..13102)
                     /gene="grxC"
                     /locus_tag="POA77_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDI6366311.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13244..13675)
                     /locus_tag="POA77_002913"
     CDS             complement(13244..13675)
                     /locus_tag="POA77_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6366312.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13920..15464
                     /gene="gpmM"
                     /locus_tag="POA77_002914"
                     /gene_synonym="pgmI"
     CDS             13920..15464
                     /gene="gpmM"
                     /locus_tag="POA77_002914"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI6366313.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAGKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15474..16757
                     /gene="envC"
                     /locus_tag="POA77_002915"
     CDS             15474..16757
                     /gene="envC"
                     /locus_tag="POA77_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDI6366314.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16761..17720
                     /locus_tag="POA77_002916"
     CDS             16761..17720
                     /locus_tag="POA77_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDI6366315.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17707..18741)
                     /gene="waaH"
                     /locus_tag="POA77_002917"
     CDS             complement(17707..18741)
                     /gene="waaH"
                     /locus_tag="POA77_002917"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDI6366316.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18980..20005)
                     /gene="tdh"
                     /locus_tag="POA77_002918"
     CDS             complement(18980..20005)
                     /gene="tdh"
                     /locus_tag="POA77_002918"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDI6366317.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20015..21211)
                     /gene="kbl"
                     /locus_tag="POA77_002919"
     CDS             complement(20015..21211)
                     /gene="kbl"
                     /locus_tag="POA77_002919"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI6366318.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21486..>22071)
                     /gene="yibB"
                     /locus_tag="POA77_002920"
     CDS             complement(21486..>22071)
                     /gene="yibB"
                     /locus_tag="POA77_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HDI6366319.1"
                     /translation="IQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMG
                     LVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMI
                     GNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNL
                     NYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5616 a         5695 c         5611 g         5149 t
ORIGIN      
        1 tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca
       61 tcactgtcat taatgtaaag caacatagct caccaataat acaatggaca actctaccgg
      121 cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc
      181 agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc
      241 aagccagaaa gtagtttact gaaacaacaa gaagaacata ataataaaat tgtgctactt
      301 gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc
      361 ataataaaca gtacaaagac tttaactcac gtaattaaat ccgttaaaga ccaacaaaat
      421 acttttattt tcaccgagtt taacccagca aaaacgaagt atttcatcct gaacaacggt
      481 tcggtggctt tagcgggtcg agtgttatcc attgacgcca cagagaatgg tagtgttatt
      541 catatctcac tggtcaactt attaagtaca cctatctcaa acattggttt taatgcgaca
      601 tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca attacttttc
      661 aacacatcaa tgaaatccac attgaaatta ttaccaggtc aatggcaaga cattaatttg
      721 accctgaagg gtgtatcgcc caataacctg gaatatctga agttagccat caacatggaa
      781 aatattcagt tcgacaatct tccctctgct gaaaatcggc agaaaagaag caaaaaataa
      841 atgcagttag tacgcttata acaaggaata gcgagcatca ttaatgaaca aaatatttaa
      901 agttatctgg aaccctgcga cagggaatta tactgttacc agcgaaacgg caaaaagccg
      961 tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt
     1021 gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg
     1081 tagtggatca gctggcgacg gctgggttgc tataggcaaa ggggcgaaag caaatacttt
     1141 tatgaacacc agtggttcca gtactgctgt gggttatgac gctatagctg aaggccaata
     1201 tagctctgcc atcgggtcaa aaacccatgc gattggcggt gcatcaatgg cctttggggt
     1261 tagtgcaata tcagaaggcg atagaagtat agcactgggt gcctcttcgt attcattggg
     1321 ccaatactca atggccctcg gccgttattc aaaagcattg ggtaaattgt ctattgctat
     1381 gggggactct tccaaagcgg aaggagcaaa cgccattgcc ctgggaaatg ccactaaagc
     1441 tactgagatt atgagtattg ctcttggcga caccgccaat gcgtcaaaag cgtattcaat
     1501 ggcgctggga gcaagtagcg tcgcatctga agaaaacgca attgccctgg ggcgtagcag
     1561 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
     1621 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
     1681 tgcgaaggca aaagggaata atagcatggc aatggggttc ggaagccttg ccgataaagt
     1741 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
     1801 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
     1861 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
     1921 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
     1981 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
     2041 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     2101 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     2161 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     2221 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     2281 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     2341 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     2401 tgtcgaagcc aataccgcca atatcgctac taataccagc aacattgcca cgaatacggc
     2461 aaatattgcc accaatacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     2521 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     2581 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     2641 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     2701 cgccaataac acttccaata ttgccactaa caccaccaac atctctaatc tgactgagac
     2761 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     2821 agctcatggc accgagacca ccagcaaaat caccaacgtt aaagatggcg acctgacgac
     2881 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     2941 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     3001 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     3061 tggcaacaat accgccaaca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     3121 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     3181 cggcggcaat gcttctgtga atactgacgg tgtgtttacc ggtccaacct acaaaatcgg
     3241 tgaaacaaat tattataacg tcggcgatgc actggctgcg attaactcct catttagcac
     3301 gtctctcggc gatgctctgc tttgggatgc caccgcaggt aaattcagtg ccaaacacgg
     3361 tactaatggt gacgcaagcg tgatcactga tgtcgcagat ggtgaaattt cagactccag
     3421 ttctgacgca gtaaacggct cacaactcca cggcgtgagc agttatgttg ttgatgcgct
     3481 ggggggtggt gccgaagtca atgcagacgg caccatcact gcgccgacgt acaccattgc
     3541 taatgctgat tacgataatg tcggtgatgc cctgaatgct atcgatacca ctcttgacga
     3601 cgctctgctc tgggatgcgg acgccggtga aaatggtgca tttagcgccg ctcacggaaa
     3661 agataaaact gccagtgtaa tcactaacgt cgctaacggt gcaatctctg ctgccagcag
     3721 cgacgcgatt aacggctcac aactctatac caccaataag tacatcgctg atgcgctggg
     3781 tggtgacgca gaagtcaacg ctgacggcac catcaccgca ccgacttaca ccattgcgaa
     3841 cgccgagtac aacaacgtcg gtgacgccct ggatgcgctt gatgataacg ccctgctgtg
     3901 ggatgagact gccaatggcg gtgctggagc ctacaatgcc agccatgacg gtaaagccag
     3961 catcatcact aatgtcgcta atggcagtat tagtgaggac agtaccgatg cagtgaacgg
     4021 ttctcagttg aatgcgacga atatgatgat tgagcagaac acccaaatta tcaatcagct
     4081 cgctggtaac accgacgcaa cctatatcca agaaaacggt gcgggtatta actatgtgcg
     4141 tactaacgac gacggcttag cgttcaacga cgccagcgca cagggtgttg gcgctacagc
     4201 tataggttat aactctgtcg ccaaaggcga tagcagcgta gctattggtc agggcagcta
     4261 cagcgacgtt gatacgggta tcgccctggg tagcagctct gtttccagcc gagtgattgc
     4321 caaaggctcc cgtgacacca gcataacgga aaatggcgtt gttattggtt acgacaccac
     4381 ggatggcgaa ctgctcggtg cattgtctat cggtgatgac ggtaaatatc gtcaaatcat
     4441 caacgtagcc gatggttccg aagcccatga cgccgttacg gttcgtcaat tgcagaatgc
     4501 gattggtgcg gtcgcaacca cgccgactaa atacttccac gctaattcaa cggaagaaga
     4561 ttcactggca gtgggaactg actcgctggc aatgggtgcg aaaaccatcg tgaatggcga
     4621 taaaggtatt ggtatcggtt atggtgccta cgtggacgcg aatgcactta acggcattgc
     4681 cattggtagc aatgcgcaag tcattcatgt caacagtatt gcgataggta atggttctac
     4741 gaccactcgt ggcgctcaaa ccaattatac cgcctacaac atggacgcac cgcagaactc
     4801 tgtcggtgaa ttctcagtcg gtagtgcgga tggtcaacgt cagatcacta acgtcgcagc
     4861 aggttcggct gataccgatg cggtcaacgt gggtcagttg aaagtaacgg atgcgcaggt
     4921 ttcccagaat acccagagca ttactaacct gaacaatcag gtcactaatc tggatactcg
     4981 cgttactaat atcgaaaacg gtattggcga cattgtcacc tccggtagca ccaagtactt
     5041 caagaccaac accgatggcg tagatgccaa cgcccagggt aaagatagcg ttgctattgg
     5101 ttctggttcc attgctgccg ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga
     5161 ggaagaaaat acaatctctg taggttcttc tactaaccaa cgccgtatca ccaacgtagc
     5221 tgccggtgtt aatgccaccg atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg
     5281 cggcgtgcgc tacgacacca aagctgatgg ttctatcgac tatagcaata tcaccctcgg
     5341 tggtggcaac ggcagtacga ctcgtatcag caacgtctcc gctggcgtca acaacaacga
     5401 cgcggtgaac tacgcgcagt tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca
     5461 gcggatggtt gagatggata acaaactgtc taaaactgaa agcaagttga gcggtggtat
     5521 cgcttctgca atggcaatga ccggtctgcc gcaggcttat acaccgggtg ccagcatggc
     5581 ttctattggt ggcggtactt acaacggtga atcggcagtt gctttaggtg tatcgatggt
     5641 gagcgccaat ggtcgttggg tctacaaatt acaaggtagt accaatagcc agggtgaata
     5701 ctccgccgca ctcggtgccg gtattcagtg gtaatgatcc attaagttag tgtgactaag
     5761 ggcaaacgcc aaattgcctg atgcgttacg cgcatcaggc aagataaggc gctccgggtc
     5821 aacaatctaa gggcaattct ctgatgagga ttgccctttt tctttaccag acatctcccc
     5881 ccacaagaat tggccctacc aattcttcgc ttatctgacc tctggttcac aattccccaa
     5941 ttaaaactca catcaatatt gccaatacat aacatttagt taaccattca ttgtcattat
     6001 ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg agatgagcaa
     6061 cagactcatt acacgatgtg cgtggactcc aggagacctg caatgaatct ctggcaacaa
     6121 aactacgatc ccgccgggaa tatctggctt tccagtctga tagcatcgct tcccatcctg
     6181 tttttcttct ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc ctcgtggacg
     6241 gtggcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc taacgcgctg
     6301 gcctcggtgg tttatggttt cttctacggg ttgtggccca tcgcgtggat cattattgca
     6361 gcggtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct
     6421 attctttcga taacccctga ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc
     6481 gcgttccttg aaggagccgc aggctttggc gcaccggtag caattaccgc cgcattgctg
     6541 gtcggcctgg gttttaaacc gctgtacgcc gccgggctgt gcctgattgt taacaccgcg
     6601 ccagtggcat ttggtgcgat gggcattcca atcctggttg ccggacaggt aacaggtatc
     6661 gacagctttg agattggtca gatggtgggg cggcagctac cgtttatgac cattatcgtg
     6721 ctgttctgga tcatggcgat tatggacggc tggcgcggta tcaaagagac gtggcctgcg
     6781 gtcgtggttg cgggcggctc gtttgccatc gctcagtacc ttagctctaa cttcattggg
     6841 ccggagctgc cggacattat ctcttcgctg gtatcactgc tctgcctgac gctgttcctc
     6901 aaacgctggc agccagtgcg tgtattccgt tttggtgatt tgggggcgtc acaggttgat
     6961 atgacgctgg cccacaccgg ttacactgcg ggtcaggtgt tacgtgcctg gacaccgttc
     7021 ctgttcctga cagctaccgt aacactgtgg agtatcccgc cgtttaaagc cctgttcgca
     7081 tcgggtggcg cgctgtatga gtgggtgatc aatattccgg tgccgtacct cgataaactg
     7141 gttgcccgta tgccgccagt ggtcagcgag gctacagcct atgccgccgt gtttaagttt
     7201 gactggttct ctgccaccgg caccgccatt ctgtttgctg cactgctctc gattgtctgg
     7261 ctgaagatga aaccgtctga cgctatcagc accttcggca gcacgctgaa agaactggct
     7321 ctgcccatat actccatcgg tatggtgctg gcattcgcct ttatttcgaa ctattccgga
     7381 ctgtcatcaa cactggcgct ggcactggcg cacaccggtc atgcattcac cttcttctcg
     7441 ccgttcctcg gctggctggg ggtattcctg accgggtcgg atacctcatc taacgccctg
     7501 ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg tctctgatct gttgctggtt
     7561 gccgccaata ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc tatcgctatc
     7621 gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa
     7681 cacagcctga tcttcacctg tatagtgggc gtgatcacca cgcttcaggc ttatgtctta
     7741 acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag gttgccgatc
     7801 gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg catgaagttg cccgctgagc
     7861 gccaactggc gatgcaactc ggcgtatcac gtaattcact gcgcgaggcg ctggcaaaac
     7921 tggtgagtga aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc
     7981 atgacacatg gtcggagcaa aacatcgtcc agccgctaaa aacactgatg gccgatgatc
     8041 cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc accgcatggc
     8101 atgcggcaat gcgcgccaca cctggcgaca aagaaaagat tcagctttgc tttgaagcaa
     8161 cgctaagtga agacccggat atcgcctcac aagcggacgt tcgttttcat ctggcgattg
     8221 ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc
     8281 aatcctcagt gaagcatagc cgtcagcgga tgtatctggt gccaccggtt ttttcacaac
     8341 tgaccgaaca acatcaggct gtcattgacg ccatttttgc cggtgatgct gacggggcgc
     8401 gtaaagcaat gatggcgcac cttagttttg ttcacaccac catgaaacga ttcgatgaag
     8461 atcaggctcg ccacgcacgg attacccgcc tgcccggtga gcataatgag cattcgaggg
     8521 agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcgc aacgcattct
     8581 gccgccgttc ctgttccact atatggatgg tggtgcatat tctgaataca cgctgcgccg
     8641 caacgtggaa gatttgtcag aagtggcgct gcgccagcgt attctgaaaa acatgtccga
     8701 cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtgg cactggctcc
     8761 ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga
     8821 cgcgcatggt attccgttta ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc
     8881 gccagccatc aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc gcggctttat
     8941 gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg acgctggttt tcaccgtgga
     9001 tatgccgaca ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg gcccgaacgc
     9061 ggcaatgcgc cgctacttgc aagcggtgac acatccgcaa tgggcgtggg atgtgggcct
     9121 gaacggtcgt ccacatgatt taggtaatat ctcagcttat ctcggcaaac cgaccggact
     9181 ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat ggaaagacct
     9241 tgaatggatc cgcgatttct gggatggccc gatggtgatc aaagggatcc tcgatccgga
     9301 agatgcgcgc gatgcagtac gttttggtgc tgatggaatt gtggtttcta accacggtgg
     9361 ccgccagctg gacggtgtac tctcttccgc ccgtgcactg cctgctattg cagatgcggt
     9421 gaaaggtgat atagccattc tggcggatag cggaattcgt aacgggcttg atgtcgtgcg
     9481 tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcttgt atgcgctggc
     9541 aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgaaaaag agatgaaagt
     9601 ggcgatgacg ctgactggcg cgaaatcgat cagcgaaatt acgcaagatt cgctggtgca
     9661 ggggctgggt aaagagttgc ctgcggcact ggccccgatg gcgaaaggga atgcggcata
     9721 gtcgtttgcg ccccctcccc ctaaccctct ccctcaggga gctgtctctt gatcagatct
     9781 cctgatcaag agacaggggg aggccggggt gaggggaaaa ggccgcactg ctcaccaatt
     9841 ttctgctatc ctgcccccgc actaaggggg cagtatgcta aacatcgtac tttacgaacc
     9901 agaaattccg ccaaatactg gcaacatcat ccgtctttgc gctaataccg gctttcgtct
     9961 gcatatcatc gaaccgatgg gatttgcctg ggacgataag cgcctgcgcc gcgcggggct
    10021 ggactatcac gagtttaccg ccgttacgcg tcatcatgac tttcgcgcgt tcctcgaagc
    10081 agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt acgcctgctc acagcgccgt
    10141 aagctatcag gatggcgatt atctgatgtt cggcccggaa acacgcggcc tgccagcgag
    10201 cattcttgat gccctgcccg ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg
    10261 cagcatgaac ctgtccaatg cggtgtcagt tgtggtgtat gaagcctggc ggcagctggg
    10321 atatccggga gcgttgttga gagattagtt actgtatgcc ggatagatga ttacatcgca
    10381 tccggcacga tcacaggaca ttagatccca tccccatact caaatgtatg gttaataccg
    10441 ttgaaatgct ggtccatatc cattgatggc ttatcgctgt ctggtttacc gacaatacga
    10501 gccggaacgc cagcggcggt tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg
    10561 ccaatcttcg cgccgcgacc aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc
    10621 atcacacctt cacgaatttt cgggtgacgg tcaccaccag atttacccgt accgccaagc
    10681 gtcacagatt gcagaatcga tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg
    10741 cccgtggcgt ggtcgagcat aatgccacgg ccaatttttg ctgccgggtg aatatcgacc
    10801 tggaacgtca cagaaacctg gttttgcaga aagattgcca atgcgcgacg cccctgattc
    10861 cacaaccagt gaccgatgcg ataggcctgc aaggcatgaa aacccttcag gtataacaac
    10921 ggggttgagt atttatcgac tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc
    10981 gcagaggcga tcatttccgg gtcagcggcg taggcttctt ccaccacttc acggatagca
    11041 atagcaggca taattggcga tgacagcttg ttcgccagca tgtagctcag tgcacttcca
    11101 aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc
    11161 gccagcgttc tggcttcggc tttaatattg ttccagacaa tttccagttc ttcacacgac
    11221 attgcttact ccacacgatg agataatgac cggcccgttc tgcgcgggcc gggtcatagc
    11281 ggtaacaaag gttccctggg gttagtggct gctgcgctcg tccttgcgtg cacgacctaa
    11341 taacgtcaat gctgcctcgc gcgcgttttt tccgcaatat aatacttgat aaatttcctc
    11401 ggttattggc atttcaacgc cgaagcgatg cgccagttcg cggacttctt tcgtattgcg
    11461 gtagccttcc accacctgac caatcttctc ctgcgcgctt tgtacatcca tgccctgacc
    11521 gagcatcatg ccaaaacggc ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc
    11581 gccaagcccc gccatgccca taaaggtggc aggatcggca cccagcgccg cgccaagacg
    11641 cgacatttca gccaggccac gggtgattag cgccgtacgc gcattcgcac cgaaaccgat
    11701 accgtcggac atccccgcgc caatggcaat gacgtttttc accgcaccgc caagctgcac
    11761 accgatgaaa tccgggttgc tgtaaacgcg gaaacttttg ccacagtgca gcaattgttg
    11821 gagatcatcg gcaaaggtct ggtcggtaga ggccaacgaa atagccgtcg gtaaacctgc
    11881 cgccagttct ttcgcaaacg ttgggccaga gataaccgcc agcggaattt gatcgcctaa
    11941 ggcctcacgc gccacgtcct gtaacagacg tccggtttcc gcttccagcc ctttggtcgc
    12001 ccacaccaga cgcgcatcag gacgcatcag cggtttaatc tggcgcagca cttcaccaaa
    12061 gacatggctg ggtacgacga cgagaatatt acggctggct gccagcgcag tggcgagatc
    12121 gctttcaaga tggagcgtat cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg
    12181 gtcgcgttca agcgttgcga tatgttcagg gtcatggccc cagaggacaa cctcgtggcc
    12241 atttcttgcc agggtgatgg caagagcggt gccgtacgag ccggcaccga tcacagtcat
    12301 tgaagcatta cgttggttca tcaggcatcc tgatgttctt cagtaccttc gccagcctgc
    12361 tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg
    12421 aatgtaccgc gggataccat gctggtgatg cactcacgag catacgggaa cagaatgttc
    12481 gggcagtatg cgcccaggca atgtgccatc tgggtgcctt cgatacccgc gatggagaaa
    12541 ataccgccct gctgaacttc acacaggaac gcggtttctt cgcccaggga ggccgttacg
    12601 gtaacacgca gtaccacttc gtatacgtca tctgccagtt gggaagatgc cgtatccaga
    12661 tcaagtttaa cttctggttg ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg
    12721 aaagagatat ccttggtata aatacgttgg atctggaaag tcatttcagt gttgttttgt
    12781 tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa
    12841 cgttcacacg ttatttcagc aggggatcca gtccaccacg tgcatccaat gcatacaagt
    12901 catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc
    12961 tgcgtttgat catctcttca cgcttggcgg cgttgccatc gatcggcagc tcctggaaac
    13021 tcacgccctt gctgctcagc agtgcttttg cacgatggca atacgggcag gtttctttgg
    13081 tatagatttc aacattggcc ataaattagc tcctgttttt ttaccctgaa atttcatgtt
    13141 gcagggcggc agcaaaatgg agtgtcccca gaagcttata acaataagtc gctgggggcc
    13201 taattgcagc taacggcctg catcatgaaa gacgacaggt aaattatttg ccgcgcacca
    13261 gaggcaagtt ttcgccagcc cagccagcaa cgccctcttt cagcacgaat acttgcgcaa
    13321 aaccagcttt cgtcagcgcg tttgcaggct cctggcactg catgccagaa ccgtctacca
    13381 cgataaccgg tttgtctttg tgcttctcaa gctcaccaac attgttggct ttgatttcgc
    13441 tcggcaacag attgatagaa cctgcgatat ggcctttacg gaagtcatca cgctgacgta
    13501 aatccacaac cacagcgtct tctttgttga tcagacgcgt agcttcacca cgagtaatca
    13561 ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag aaccgccacc agtaacgcga
    13621 tccaggcgat actcagtatg ggatgacggc caacaaattg cataatttct tgcatggggg
    13681 gtaacaactc ccgacgtagt gattaaaaaa accaggaaag gagtatacct gcgcggtgcg
    13741 gcaaatacag ccagcgcgtt aactggaatg caattttgcg gggcacgacg aaaaaaaagc
    13801 gcaaatctgg catcggcttg ccgcgagcgg tcgtatattt tgatcttcag aggctatttt
    13861 atcgattcag ctgtagtaca attacgcaaa ttttgactct tgagtatgag gttgtcgcaa
    13921 tgtcggtttc taaaaaacct atggtactgg tgattctgga tggctatggc tatcgtgaag
    13981 aacagcagga taacgccatt tttagtgcta aaaccccggt aatggatgca ctgtgggcca
    14041 atcgtccgca taccctgatc gacgcttccg gtctggaagt cggtctgcct gaccgtcaga
    14101 tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc
    14161 tgactcgtct ggacgttgaa atcaaagatc gcgctttctt tgctaatcca gtgctgactg
    14221 gcgcggtaga taaagctaaa aacgcaggta aagcagtaca cattatgggt ctgctctccg
    14281 caggcggcgt acacagccac gaagatcaca tcatggcgat ggtagaactg gcagctgaac
    14341 gcggcgcagg aaaaatctac ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca
    14401 gtgctgaatc ctcgctgaaa aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg
    14461 tagcgtccat cattggtcgt tactacgcga tggaccgcga taaccgttgg gatcgcgttg
    14521 aaaaagccta tgacctgctg actctggcgc agggtgagtt ccaggccgat actgccgttg
    14581 ctggtttgca ggcagcttat gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta
    14641 ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg cgatgcgctg attttcatga
    14701 acttccgtgc tgaccgcgcg cgcgaaatca ctcgtgcatt cgtaaatgct gatttcgacg
    14761 gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat gctgaccgaa tacgctgccg
    14821 acatcaaaac cgcagtggct tacccaccag cttccctggt taacaccttc ggcgagtgga
    14881 tggcgaaaaa cgacaaaact cagctgcgta tttccgaaac cgaaaaatat gctcacgtca
    14941 ctttcttctt taacggtggc gtagaagagt cgttcaaagg cgaagatcgc attctgatca
    15001 actcaccgaa agtggctacc tacgatctgc aaccggaaat gagctccgca gagctgaccg
    15061 aaaaactggt tgcggccatc aagagcggca aatacgacac catcatctgt aactatccga
    15121 acggcgacat ggtaggtcac accggggtga tggaagcggc ggttaaagcg gttgaagcac
    15181 tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc cgtgggtgga caactgctga
    15241 tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca
    15301 cagcacacac caacctgcca gttccgctga tttacgttgg tgataagaac gtgaaagcgg
    15361 ttgcaggcgg caaactttct gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa
    15421 tcccgcaaga gatgactggt aagccgctgt tcatcgtgga ataatccctc cccatgaggg
    15481 gaaaggcgat taataccatg acacgggccg tgaaaccgcg caggtttgca atcaggccca
    15541 tcatctacgc cagcgttctt agcgctggcg tattgttgtg cgccttttcc gcccacgcgg
    15601 atgagcgtga ccaactcaaa tctattcagg ccgatatcgc cgcaaaagag cgcgcggtac
    15661 gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga
    15721 tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac
    15781 agattgatga gatgaacgcg tcgattgcca aactggagca gcaaaaagcc gcccaggagc
    15841 gcagcctcgc cgcgcaactg gatgccgcat tccgtcaggg cgagcatacc ggtattcagc
    15901 taattctcag cggtgaagaa agccagcgtg gacagcgttt acaggcttat ttcggctatc
    15961 tcaaccaggc gcgacaagaa accattgccc agttgaagca aacgcgtgaa gaagtcgcca
    16021 tgcagcgtgc tgaactggaa gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc
    16081 gcgcccaaca ggcgaaactg actcaggcgc tgaacgagcg taaaaagacg ctggcagggc
    16141 tggagtcttc catccagcaa ggtcagcaac agttgagcga gctgcgcgcc aacgaatccc
    16201 gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa agcgcgtgca gaacgagaag
    16261 cacgtgaggc ccaggcggtt cgcgaccgcc agaaagaagc gacgcgtaaa ggcaccacct
    16321 acaaaccgac tgaaagcgaa aaatcgctga tgtcccgtac cggtggtctg ggcgcgccgc
    16381 gtggtcaggc attctggccg gttcgcgggc cgacgctgca tcgctatggc gaacagctac
    16441 agggtgaatt acgctggaaa gggatggtta tcggtgcctc tgaaggtact gaagttaaag
    16501 cgattgccga cggtcgggtg attctggctg actggctgca aggttacggt ctggtggtgg
    16561 tggttgagca cggtaaaggc gacatgagtc tttacggcta taatcagagc gcactggtga
    16621 gcgttggttc gcaggttcgc gcgggccagc caattgcact ggtgggcagc agtggcggtc
    16681 agggtcggcc ttcactctat ttcgaaattc gccgccaggg tcaggcggtc aatccacagc
    16741 cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc
    16801 tgttggcgct ctcctccccc gtacttgctg gcaaacttgc catcgtcatt gatgattttg
    16861 ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc ctccgctatc tccgtcgctg
    16921 tattacccga ttcaccgcac gccagagaga tggcgaccaa agcgcataac agcgggcacg
    16981 aagtgttgat tcatctcccg atggcaccac tgagtaaaca gccgctggag aaaaatacgc
    17041 tgcgcccgga gatgagcagc gacgaaattg agcgcattat tcgtagtgcg gtcaataacg
    17101 tgccctatgc cgtggggatc aacaaccaca tgggtagcaa gatgacctct aacctgtttg
    17161 gtatgcagaa agtgatgcag gcactggagc gttacaatct ttacttcctc gacagcgtaa
    17221 ccatcggtaa tacccaggcg atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac
    17281 ggaaggtgtt cctcgacgat tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg
    17341 caattgatct ggcgcgtcgc aacggttcaa ccattgccat tgggcatcct cacccttcaa
    17401 cggtacgcgt gctacaacag atggtttata acctgccgcc agacattacg ctggtgaaag
    17461 ccagcagctt gctgaatgaa ccgcaggttg atacttctac accgccgaaa aacgctgtgc
    17521 ctgatgcacc gcgtaatccg ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag
    17581 aacctgttta tgctaatcgc ttctttgaag tattaagcga aagcatcagc cagagcacgc
    17641 tgatcgttta cttccagcat cagtggcaag gctggggcaa acagcccgaa gcggcgaagt
    17701 ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc
    17761 gcgccattgc cataagcgga aagaccacag taacgcctgg taaccgacct tcacgctgcg
    17821 cacattggta atcaggcgct tatacatacc ggaagtaaat atctcggcaa tcatccgttg
    17881 gcgggtaaga atatccggct ctttgcgcac cgcatggcaa acacgcaatg cttcgtaagt
    17941 tatttgctga tgaaattccg gataaatcgt aattttgtcg gcataatttc gatttaattt
    18001 ctccagcagg cgggtaatct taatatagtg acgttgataa ttaaggtttt tattcccttg
    18061 tctatgcaac cgactcactg acgtattatg cagataatat ttatataatg attgctcggt
    18121 atatcgcgca cgcagcgcgt taaacatgaa ttctgttgtc cagacaatat cctgatgatg
    18181 taatccggca ataaatttaa tgttattttt aacaataaca tcacggcgat aaacccccat
    18241 ccagacaacg tgagtccaac ggcgcgaaga aagccccatc cgcagccagt ccgggccggt
    18301 taatacgccg gttgagcgaa ggcgatcgct ggggatggat tgccaggttt ctcccgtttc
    18361 acgaaaacac cagtcagcgt tgcactgcgc cacgtcgagg tcatcctcta acgccatggt
    18421 catcagcgtt tcgtacatgg tgggatagac ctcatcgtca gcatcgacaa aagcgacata
    18481 tttgcccgtc gccacctcga tcccacgatt acgcgctacc gatgcgccag cattcgcctg
    18541 atgcaacaaa cgaacgtgcg gatagttttc tgcgtaatgc tttgctattt caacagaatt
    18601 atccgttgaa ccatcgttaa taataatgat ttccagagca gtccaggttt gcgtaattaa
    18661 agattccata caagtgcgga aatcatcgcc cgcattatat aacggaataa taacactaag
    18721 tttattggtg ctgttcatca taaattaccg acgacctctg aagttatcct gacgttttac
    18781 aggctttgta ttaagaaact attaagcctg tgagatatta tttttacagg tgagagagtc
    18841 acttcagggt agagagataa tgagagcagc tatataagag atcagcgcaa ttacgatatc
    18901 tttattacac tgacaggatt ttacatattt aacggattat cctcaggcaa aagggctgga
    18961 ataccagccc ttgttcgtgt taatcccaac tcagaattac tttcccggac tggcccgaac
    19021 gcatagcgtc aaagcccttc tggaaatcat cgatagagaa acgatgggta atgatcggcg
    19081 agagatccag acctgactga atcagcgccg ccatcttgta ccaggtttca aacatctcac
    19141 gaccgtaaat acctttaatg aacaagcctt taaagattac tttggtccag tcgatagaca
    19201 tatcggacgg tggaataccc agcatcgcaa tgcgaccgcc gtggttcatg gtgtcgagca
    19261 tggtacgaaa cgctggcggc gcgccggaca tttccagacc gacatcaaag ccttcggtca
    19321 tgcccagttc tgccatcaca tcattaaggt tttctttagc gacgttgacc gcacgggtga
    19381 tacccatttt gcgcgccagc tcaaggcggt attcgttaac atcggtgatc accacattgc
    19441 gtgcaccaac gtgtttcgcc accgccgcag ccataatacc tatcggacct gcaccagaaa
    19501 ccagcacatc ttcgcccacc agatcgaacg acagcgcggt atgcacggcg ttaccgaagg
    19561 gatcaaaaat ggaagccagg tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg
    19621 ccggaatcac cagatattcg gcgaagcagc caggacggtt aacaccgaca ccaatggtgt
    19681 tgcggcacag atgggtacga ccgccacgac agttgcgaca gtgaccgcag gtgatatggc
    19741 cttcgccaga aacgcgatca ccgattttga agcctttcac ttcctgacca atacctacca
    19801 cttcaccgac atattcatgg cctacgacca tcggcaccgg gatggttttt tgcgaccact
    19861 catcccagtt atagatgtga acgtcagtcc cgcagatggc tgttttacgg attttaatca
    19921 gcagatcgtt atgcccaagt tccggtacag gaacgtcggt catccagatg ccctcttccg
    19981 ctttcagttt ggataacgct ttcatctcac atcctcaggc gataacgccc agttgtttac
    20041 caatacgcgt gaatgcttct actgcacgcg taatttgctc aggggtatgc gccgcagaca
    20101 tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt
    20161 aaatgccctc tttttgcagc tcacgggcaa atttctgcgc cactactgca tcgccaagca
    20221 tgaccggaat aatggcatga tcggctcccg ccagggtaaa gcccgccgcc gacatttgct
    20281 cacggaactg gcgcgcgttc gcccacagac ggtcacgcag ttcgctgccc gcttcgacca
    20341 tctccagtac tttgatggac gcggcaacaa tggccggtgc cagcgagttg gagaacaggt
    20401 acggacgaga acgctggcgc agccactcaa ccacctcttt gcgcgccgcg gtataaccac
    20461 cagaagcccc gcccagcgct ttaccaagcg taccggtgat aatatcgacc cggcccatca
    20521 catcgcagta ttcatgggaa ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt
    20581 cgtctaccat caccagggca tcgtatttat ctgccagatc gcaaacgccc ttcagattgg
    20641 caatcacgcc gtccatcgag aatacaccat cggtggcaat cagcacatga cgcgcaccgg
    20701 cttcacgcgc ttctttcaga cgagcttcaa gctcctgcat atcgttgttg gcatagcgat
    20761 agcgtttagc tttacacaga cgcacaccat caataataga cgcgtggttc agtgcgtcgg
    20821 agataatggc gtcttccgcg cccagcagcg tttcaaacag gccaccgtta gcatcaaagc
    20881 aggaagagta gagaatcgca tcttccatcc ccaggaaggc cgccagtttt tgttcaagct
    20941 ctttatggct gtcctgagtg ccgcaaataa aacgcaccga agccatgccg aaaccgtgag
    21001 aatccattcc cgcctttgcc gccgcaatca gatcaggatg attcgccagc ccgagatagt
    21061 tgttggcaca aaagttaatg acgtggcttc catcagccac agtgatatct gcttgctgcg
    21121 cagacgtaat aatgcgctct tctttaaaca acccttccgc ccgtgcggtt tccagatcgt
    21181 tggttaactg ctgataaaat tctccacgca ttgcgattct ccagacaggg caaattccag
    21241 cacatattac ccaaacttat aggtcgcgac gagatgacgc gttaacactt ctgcaaaatt
    21301 caggataaat aacgtgtaac ttgatgtctc tcaacaccta cagatagtag aaaaactgta
    21361 cagttttggc tgggcataat gatgattgat tttactgcat tgatataaaa agaaaaaatg
    21421 atgccaaagc atcatttttc ctatctcagt tggtatcaaa acgctaccca ctaatggagg
    21481 gaaacctatt ttcttgaaag gaaaattctc agcgcctgca tttttgcccc taaagtgtta
    21541 ctcctgaagc agcgaaacaa atcgaaccat tttcctctcc ccagatagtt taggttgaag
    21601 agatcggggc gtttgtaata gcacatgaca aaaatgccct ggtcatcatc cacaatgtta
    21661 ttatttagtg ttattttttg actttccaga actaatttat agaattcttt ccatttatgc
    21721 tgtgaaccaa caattgcgcc cccaataata tatacatgat tgccaatcat gaaatcaaaa
    21781 acctgttgtt gagatgtgac agtaagccct ttttttatag tgaaaagatg catcttactt
    21841 tcatcaaatg ggaaatccca aattttcaat cctcgagtta catttggctt acggcaatag
    21901 ccaaaatcta tccacgcaac aagaggcgtt ttcactaagc ccatgttgat agctttattt
    21961 acaaaatagg ccttaaggtt acataccaaa acatactctg gtgaccagta ctctgggttt
    22021 tttaattgtc gaggctcaag tctatttgta aacgactcat ctttttgaat t
//
LOCUS       DAMZII010000079        21873 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZII010000079 DAMZII010000000
VERSION     DAMZII010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.079"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            326..1633
                     /locus_tag="POA77_002921"
     CDS             326..1633
                     /locus_tag="POA77_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6366320.1"
                     /translation="MKVNYPLNKITQVLDEKSITAAHKNSFNVDVEAQGYSDSGVSDV
                     VEYPGVAPKNTGGVFTKALFDEYKIRAVQAAQTIETSDKKLTGLKAIFYPSGRIKFIV
                     RKSIRGKNIYVAVGGYPEISIKDAREHAFKIIQELKTAVEAYGERFLTHSKMTFNELV
                     DEYEKSVLSTGVKRSANTDLSKIRKYLRPLLGDKKLAGMTEADILSYLHNLDLKPATR
                     NRHLALIKAVFTWAIRMGYISRSPALYIKALPEVTSDRRAMDDSQLQRWMDVCERWRN
                     GKPDHEALALLMFLALTGLRLGETRHLRLEDVDWSRKVITLRHTKNGKLRRVPVCDKA
                     FVLLTEQRQHLGDEGWVFPGKGTDNPVAEPRRLQKRLCEAAGIPPFTIHEMRHTFATK
                     LIESGADIHTVKDLLGHSTIKVTELYLHASPARYHEVVNRAMG"
     gene            1718..3631
                     /locus_tag="POA77_002922"
     CDS             1718..3631
                     /locus_tag="POA77_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6366321.1"
                     /translation="MNKKVTTNVPKVSGIKRIRAFADRDKNFNKMSEAIIRRAENTEV
                     GEDDIASLGIGDGLPRHYVNTADGLFYIGGQKPVQVCTTPVVPVALFCGKDKESGLGI
                     SLMTGNGLGKYVKAQVSFEDIVQGGAQVMGRLAGRGFCVGPSPQQLALFDRFIQSCRS
                     LPLPVYLMADAMGFVSEDMAFLHGHEPVVTSLTGFDYLLPGFRVRPGLHKGGALEEWI
                     NLIRENVHGWAQTFALSASFASMLLVPAGMDVGMFHFHGVSTTGKTLLLMLAASVHGD
                     GSEPGSGGNVNIIRWNTTPNAMERLLADYSGLVACVDELGASNHKQLSSLLYNITSGT
                     AKERLNRGLTADEPFKWRMFILSTGEMSISEKLAEQKAVLQGGQEHRAISMELLPEDA
                     QKPDEPVSETRARADALKTGLAEAYGHAGPAFIGKLLSGKNTAGGLRTYAEFSSWLQE
                     QTDAYCNALEEALRDEGRDITRVQRRGLKRFALVWLAGEKALKYGLLPFESDAVAESV
                     MSAARRWLDDNQNQYNPVRNMLEELQLTLVTKSSRHFITLRDEHARCPGDMWGFVHYK
                     TGDFLIYESVFEKLCSEKGLKTVDVARPLEHSGYLLGESAGHYKKRALKTMAQSYYQV
                     KKAFLKCNVMTEF"
     gene            3681..4037
                     /locus_tag="POA77_002923"
     CDS             3681..4037
                     /locus_tag="POA77_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDI6366322.1"
                     /translation="MIFESDQYYDGFPHAISSENEEKTSFNDYALVNTLAECLYHDIS
                     REIFDGSEPALRAVIANGQIKFLARYTLRGACYYEPLGRFPRMSIKDARECARELYNR
                     YDDNRVPDEEYGAIPF"
     gene            complement(4092..5276)
                     /locus_tag="POA77_002924"
     CDS             complement(4092..5276)
                     /locus_tag="POA77_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6366323.1"
                     /translation="MSTPKRFRFTNQLIKSLPPNTSDARSTDAEYSDTEISGLKCLVS
                     RREGRKKFLLRYLYHGRKRAIAIGHWPEVDVNLARQIAIEHKRSLATGTDPKQERENK
                     RMELTFDELFNQHYLPWAKSAKRSWGKDVQRYRDHIRPAIGQRLLSDITPASILRLQQ
                     TLSTSLSAATCNRVIVLIKAVFTWAGRQSMTSVHPARVVQLLRENNARQRYFTEDEIR
                     RIFLSADNDTSPVAARYVKLLLLTGLRRDELRLAKWEHVDPVKRTLWLPETKNGYGRI
                     VHLNSLAMDVIRTLPTQRGNPWLFIGKKPGMPLSNPVKAFQRIVRRAGIFDKEVCIHT
                     CRHSVAALIVSYGGTLYDVQAQLGHRSSQSSQRYAHLHPQRLQNTSQLLAERITAQLP
                     SL"
     gene            complement(5622..6209)
                     /locus_tag="POA77_002925"
     CDS             complement(5622..6209)
                     /locus_tag="POA77_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366324.1"
                     /translation="MDTHTPYNCNDIARLALTMHGHSYFFSLRRHLNINFSRDLNGSG
                     TQGLFIKKQNVDIDLIKVIFDYTDNKNDDFLYEADLIKDQRKDYEPTVNRGKHRFVAK
                     QIELNIDWNGNEIQQWRADIERLTRSHDNLEDWLKNGSEMLVCCASGFFCRLPTILTL
                     NDLKQYVAMGVTLEDLKTRLKCSKCGKRGSKVTVF"
     gene            complement(6924..6999)
                     /locus_tag="POA77_002926"
     tRNA            complement(6924..6999)
                     /locus_tag="POA77_002926"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6964..6966),aa:Phe,seq:gaa)
     gene            complement(7105..7812)
                     /locus_tag="POA77_002927"
     CDS             complement(7105..7812)
                     /locus_tag="POA77_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDI6366325.1"
                     /translation="MVIGPFINASTVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            8210..10345
                     /gene="speC"
                     /locus_tag="POA77_002928"
     CDS             8210..10345
                     /gene="speC"
                     /locus_tag="POA77_002928"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDI6366326.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(10395..11651)
                     /gene="nupG"
                     /locus_tag="POA77_002929"
     CDS             complement(10395..11651)
                     /gene="nupG"
                     /locus_tag="POA77_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDI6366327.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11853..12932)
                     /gene="mltC"
                     /locus_tag="POA77_002930"
     CDS             complement(11853..12932)
                     /gene="mltC"
                     /locus_tag="POA77_002930"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDI6366328.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGAEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(12997..13272)
                     /locus_tag="POA77_002931"
     CDS             complement(12997..13272)
                     /locus_tag="POA77_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDI6366329.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(13300..14352)
                     /gene="mutY"
                     /locus_tag="POA77_002932"
     CDS             complement(13300..14352)
                     /gene="mutY"
                     /locus_tag="POA77_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI6366330.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            14513..15232
                     /gene="trmB"
                     /locus_tag="POA77_002933"
     CDS             14513..15232
                     /gene="trmB"
                     /locus_tag="POA77_002933"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6366331.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            15232..15558
                     /locus_tag="POA77_002934"
     CDS             15232..15558
                     /locus_tag="POA77_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDI6366332.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            15742..16461
                     /locus_tag="POA77_002935"
     CDS             15742..16461
                     /locus_tag="POA77_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDI6366333.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            16637..17683
                     /gene="ansB"
                     /locus_tag="POA77_002936"
     CDS             16637..17683
                     /gene="ansB"
                     /locus_tag="POA77_002936"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDI6366334.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            17800..18807
                     /locus_tag="POA77_002937"
     CDS             17800..18807
                     /locus_tag="POA77_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDI6366335.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(18863..20164)
                     /locus_tag="POA77_002938"
     CDS             complement(18863..20164)
                     /locus_tag="POA77_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDI6366336.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGIM"
     gene            complement(20164..20667)
                     /locus_tag="POA77_002939"
     CDS             complement(20164..20667)
                     /locus_tag="POA77_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDI6366337.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(20712..21698)
                     /locus_tag="POA77_002940"
     CDS             complement(20712..21698)
                     /locus_tag="POA77_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI6366338.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         5706 a         5114 c         5578 g         5475 t
ORIGIN      
        1 gggttgtttt ttagtcgaaa atcggtgggg tctattacca ttttcttatt tcagggcctc
       61 aaaagttgac ttactttggg cgatgcatcc ggaacacaat ttttttatag ggtttaacgc
      121 tattttccct tttccctgag caggagtact cctgatgaat catcatggtc tttctgactt
      181 gagtattaaa tgtcaatggg catgtaagag ctgatttttc atgggcttga aagtgatgcc
      241 acgaataaat aagcttatcc cattgttggc cgacatatta aattactcat tatcatctga
      301 attttcagag gaccatgtac atattatgaa agttaattac cctttaaata aaataacgca
      361 agtgctggat gaaaagagca tcacagctgc tcataaaaat tccttcaatg tagatgtgga
      421 agctcagggg tattccgact ctggagtatc tgatgttgta gaatatccag gggttgcacc
      481 taaaaatact ggaggtgttt ttactaaagc cctttttgat gaatataaaa tccgtgcagt
      541 acaagctgca caaacaatcg agaccagcga taagaagtta accggactta aggcaatatt
      601 ctatccatca ggtcgcatta aatttattgt ccgcaaaagt attcgcggaa agaatatcta
      661 cgttgccgta ggaggctacc cggaaatttc aattaaagat gcaagggagc atgcctttaa
      721 aataattcag gagctcaaga ccgccgttga ggcttacggt gagcgttttc ttactcacag
      781 taaaatgacg tttaatgagc ttgtggatga gtatgagaaa agcgtactca gcacgggcgt
      841 taagcgttca gctaacaccg acctttctaa gatccgtaaa tacctgcgcc ctcttctcgg
      901 tgacaaaaag ctggcaggta tgacggaggc agatattctg agctatctgc ataatctcga
      961 tctcaagcct gccacccgca accgccatct ggcgctcatt aaagcggtgt ttacctgggc
     1021 tatacgcatg ggatacatca gccgctctcc ggcgctttat atcaaagcgc tgcctgaagt
     1081 gacgtcagat cgccgtgcca tggatgactc ccagcttcag cgctggatgg acgtctgtga
     1141 gcgctggcgt aatggtaaac ccgatcatga agcccttgct cttctcatgt tccttgccct
     1201 gacggggctg cgtctggggg aaacccgtca ccttcgtctt gaagatgtgg actggagccg
     1261 caaggttatc acccttcgcc ataccaaaaa cggcaaacta cgccgtgtac cggtatgtga
     1321 taaggctttc gttcttctga cagaacagcg tcagcatctg ggtgatgaag gctgggtatt
     1381 tccgggcaag ggaaccgaca atccggttgc tgagccgcgt cgcctgcaaa aacgactttg
     1441 tgaggctgcg ggtattccac cgttcaccat ccatgagatg cgtcatacct ttgcgacaaa
     1501 gctcattgag agcggggccg acattcacac cgtcaaagac ttattgggtc acagcactat
     1561 caaagtaacg gagctttact tgcacgcttc cccggcgcgt taccacgaag tcgttaaccg
     1621 ggcgatgggg taacaacctt cactcaacgg tatggttttt aactatggtt ctgatgcacc
     1681 cgttttctga cggtggcgta atttgatggt aatacacatg aataaaaaag tgacaactaa
     1741 tgttcctaag gtttccggga ttaagcgtat tcgcgctttt gctgaccgcg ataaaaattt
     1801 caacaaaatg tcggaagcaa ttatcaggcg tgctgaaaac actgaggtcg gtgaagatga
     1861 tatcgcttca ctcggtattg gtgatgggct gccacggcat tatgtgaata cggctgatgg
     1921 ccttttctac atcggtggac agaaacctgt gcaggtttgc accacgccag ttgtgcctgt
     1981 cgccctgttt tgtggaaaag ataaggagtc cggtcttggg atcagcctga tgaccggtaa
     2041 cgggctgggt aagtatgtta aggcccaggt gagctttgag gatattgttc agggtggtgc
     2101 tcaggttatg ggcagactgg caggtcgtgg attttgtgtt ggcccctctc cacagcagct
     2161 tgctcttttt gaccgtttta ttcagagctg ccggagttta ccgctgcctg tctatctgat
     2221 ggcagatgca atgggattcg tttcggagga tatggccttt ttacatggtc atgagcccgt
     2281 tgtgacctct ctgaccgggt ttgattatct gctgccggga ttccgcgtgc gtcccggact
     2341 gcacaaaggt ggggctctgg aggagtggat taacctcatc cgggaaaatg tccatggctg
     2401 ggcgcagacc tttgcgctga gtgcgtcctt tgcttcgatg cttctggttc ctgccggtat
     2461 ggatgtgggc atgtttcatt ttcacggcgt atccaccacc ggtaaaacgc ttctcctgat
     2521 gctggctgca tcggtccatg gtgatggcag cgagccgggt agtgggggga atgtaaatat
     2581 tattcgctgg aataccacgc cgaatgcgat ggagcgtctt ctcgctgact attccggtct
     2641 ggtcgcctgc gtcgatgaac tgggcgcgag taatcataag caactttcct cactgcttta
     2701 caacattacg tcaggtacgg caaaagagcg cctgaaccgt ggcctgacgg cagatgagcc
     2761 gtttaaatgg cgcatgttta ttctctctac cggtgagatg tcaatctccg aaaagctggc
     2821 tgaacagaaa gccgtgttac agggagggca ggagcacagg gccatcagta tggaactgct
     2881 gcctgaggat gctcaaaaac cggatgaacc ggtatcagaa acacgtgccc gtgcagatgc
     2941 actgaagacg gggctggcag aggcttatgg tcatgccggg cctgcattta tcggcaaact
     3001 gttatccgga aaaaatacgg ctggcggtct tcgcacctac gcagagtttt catcctggct
     3061 ccaggaacag acagatgcgt actgtaatgc actggaagag gccctgaggg atgaggggcg
     3121 tgatattacc cgcgttcagc ggcgcggact gaaaagattt gcgctggtat ggctggcagg
     3181 agagaaggcg ctgaaatatg gccttttacc tttcgaaagc gatgcggtgg ctgaatccgt
     3241 tatgtccgct gcacgacgct ggctggatga taaccagaat cagtataacc cggtgcgtaa
     3301 catgctggag gagcttcagc tgacgctggt gacaaaaagc agcaggcact ttatcaccct
     3361 gcgtgacgaa catgcccgat gcccgggaga tatgtgggga ttcgtccatt acaaaaccgg
     3421 cgacttcctg atttatgaat cggtgtttga aaagctctgt tctgaaaagg gacttaagac
     3481 cgttgatgtg gccagaccgt tagagcattc cggttatctg cttggcgaaa gtgccggaca
     3541 ctataaaaaa agagcgctga agacgatggc gcagtcttac taccaggtca aaaaagcgtt
     3601 tctgaaatgc aatgtgatga ccgaatttta accctgaata aaaccatttt gactgaataa
     3661 caacgaatag aaggtgatgt atgatttttg aatcagacca gtactatgac ggttttcctc
     3721 acgctatcag cagcgaaaat gaagaaaaaa cctcattcaa tgattacgct ctggttaaca
     3781 cactggcgga gtgcctgtat cacgacattt ctcgtgaaat cttcgatggc agtgagccgg
     3841 cgctccgggc cgtcattgcc aacggtcaga ttaaattcct ggctcgttat acgcttagag
     3901 gggcctgcta ttatgaaccc ctcggcaggt tccctcgtat gtctattaaa gacgcgcgcg
     3961 agtgtgctcg tgaactctat aacagatatg atgataaccg ggtgccggat gaagaatatg
     4021 gggccatccc gttctgactg atggctgaac agtaaaaaaa agcaggggca cttgcctctg
     4081 cttttctgtt atcacaagga cggtagctga gccgttatcc tttccgccag taactgactg
     4141 gtgttctgta agcgctgagg atgaagatgc gcataccgtt gtgaagactg actgctgcgg
     4201 tgccccagtt gagcctgaac gtcatacagt gttccgccgt aggacacgat gagggcggca
     4261 accgaatggc gacaggtatg aatacatact tctttgtcga aaatccccgc ccgacgaacg
     4321 atacgctgaa aggccttaac cgggttactc agcggcatcc ccggtttttt gcctataaac
     4381 aaccacggat ttccccgctg cgttggcagc gttctgatta cgtccatcgc cagtgagtta
     4441 aggtgaacaa tccgtccata gccattcttg gtttcaggga gccacagggt gcgtttaacc
     4501 gggtcaacat gctcccactt agccagacgc agctcatcac gacgaaggcc ggtcagtaac
     4561 agcagtttga catagcgggc agctacaggg ctggtatcgt tatcggcgga gagaaaaata
     4621 cgacgaattt cgtcctcggt aaaataacgc tgtcgggcgt tgttctccct gagtagctgt
     4681 accacccttg ccggatgaac agaggtcatg ctttgccttc cggcccatgt aaagacggct
     4741 ttaatgagta cgatgacacg gttacaggta gccgctgaca gggaagtact taacgtctgc
     4801 tgaaggcgca gaatgctcgc tggtgtgata tcgctcagca gcctctgacc aatggcggga
     4861 cggatatgat cgcggtagcg ctgaacatcc tttccccaac tgcgtttagc ggattttgcc
     4921 catggcagat aatgctgatt aaagagttca tcgaacgtca gctccattct cttgttttca
     4981 cgctcctgct tagggtcagt gccggtggca agggaacgtt tgtgttctat ggcaatctga
     5041 cgagcaagat tgacgtccac ttcaggccag tgaccaatgg cgatggcgcg ttttcgtcca
     5101 tgataaaggt agcgtagcaa aaacttttta cgaccttctc ttctgctgac cagacacttg
     5161 aggccggaaa tctcggtgtc gctgtattcg gcatcggtac tgcgtgcatc ggatgtgtta
     5221 gggggcaggg atttaatgag ctgattagta aaacggaagc gtttgggggt ggacatataa
     5281 cctcctgtga ctggtgtaaa gaacgtcagg gttgccccat ccccggcacg gcgctgttaa
     5341 tggctgagtt gattttctgg tgtggcgaag ggaatgctgg tgccacatgg gatgaatgaa
     5401 gatgggagat gcagagagag caggcgttac ggtctgcttc tggtcttaag gggaagattg
     5461 ccgttgggga agagttcaca gaaataatat gtgtttcagt cgggtataat tacatgtgat
     5521 aagggcaaag agcgtgcttt gctaaaattt gacatgttga agtccactct ttttaacgat
     5581 gtcgttatgg acgacatggc gagtcttgct tagataatta atcaaaatac tgtgactttg
     5641 gacccacgtt taccacattt cgagcacttg agccgagttt taaggtcttc tagagttact
     5701 cccattgcaa catactgctt aaggtcgttc aaagtaagga ttgtggggag acgacaaaaa
     5761 aatccagagg cgcaacatac cagcatctcc gagccgtttt ttaaccagtc ctcaagatta
     5821 tcgtgcgatc tcgttagtcg ttcgatatcc gctctccact gctggatttc attaccgttc
     5881 cagtcgatat ttagctcgat ttgtttggca acgaaccggt gtttacctct gttaactgtg
     5941 ggttcatagt ccttacgttg atctttgatg agatcagcct catagaggaa atcatcgttt
     6001 ttgttgtcgg tataatcaaa aattacctta attaaatcaa tatcaacgtt ttgttttttg
     6061 atgaataaac cctgtgttcc gcttccgttc aagtcccggc taaagttaat gttgagatgg
     6121 cggcggagtg agaagaaata gctgtgaccg tgcatagtca atgcaagtct ggctatgtca
     6181 ttgcagttgt agggtgtatg tgtatctatg atgttacctt taattcatat tcggtcgaga
     6241 gcgccaggaa actaacctaa tatacctttt ttctacacca gtatttggat caattttcat
     6301 ccctttggcc tgcttcttgt tgattcgctc tcagcgatgt ttgaaatagg ccatcagtgg
     6361 tcagaatcat tttttacttc cactttttgc tcagatttga ccctaaggtc taagttccaa
     6421 tacgtttcgc tcaattacta tcacccctga taaattattc tatgatgacg tggagggcgg
     6481 gggattcagg caatgttaag aaatgaaagg tttaaatgcg aatattacag gtggaggagg
     6541 tggtggatac ccccgactga caaggggagt tgatggcgct gagttcaccc ccttgcctga
     6601 agaggagtct ccccggtctt cccccggggg gggtggcagg taatctgttg ttaaataaag
     6661 taaattatga aatcccccat ttcccccgta ttctctgctt cattatgaat aatcttagag
     6721 aaaggaaccg taaaggaccg tagaaagacc gtagctgtga ggtgagggaa ggcacatcag
     6781 aggatgttga ggtgtctaaa tgtaacgaac atctgcaaaa atggtgaaaa atccagcaag
     6841 aaaataacgt gttgcaacga ccagaaatct accgtagcta agaaaggaaa aagccccgct
     6901 tttcagcgag gccttatgaa tattggtgcc cggactcgga atcgaaccaa ggacacgggg
     6961 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     7021 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     7081 actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     7141 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     7201 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     7261 cggtgtggtc agcggtaata tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     7321 taatgggata ctaattaccg acaccgcaat acccagcgag caggcgaaga tcatcgccgt
     7381 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     7441 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     7501 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     7561 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     7621 tagcgtagct aaaaccatcg ctggaaggtt ggcgcatttc acaaccagta aaataccaat
     7681 ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt ccggtaagcg
     7741 ttggctgagc agtgcgccga gaacgccacc cagtaagact gtactagcgt tgataaaagg
     7801 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     7861 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcttta gcgaaaggtg
     7921 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     7981 tacattctct cgcttatcgc cgtttcgcgc gaaactcttc tctttttatg ctactgctca
     8041 tgcggtgaag ttaacgcacg ttcactgcag gacaacagta aaatcagagc gtttctgctt
     8101 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     8161 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     8221 gaatattgcc gccagtagtg aactggtatc ccgactttct actcatcgcc gcgtggtggc
     8281 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     8341 cagtggcatt cttacgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     8401 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     8461 ttggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     8521 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctgggcatca
     8581 acatggtgcg ttttttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     8641 gaacgtcttt cgcgccgata tgtgtaatgc tgacgtaaaa ttgggcgatc tgcttattca
     8701 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     8761 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     8821 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     8881 gattcaggcg ggggcgacgc cggtctatct ggaagcttca cgcaacccgt ttggtttcat
     8941 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     9001 tgcgccagaa aaagccgacc tgccgcgccc gtttcgcctg gcgattattc agctgggaac
     9061 ctatgacggc actgtctata acgcccgtca ggtgatcgat accgttgggc atctgtgtga
     9121 ttacattctg tttgattccg cgtgggtcgg ttatgaacaa tttatcccga tgatggcgga
     9181 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatttttg tgacccagtc
     9241 ggtgcacaaa cagcaggctg gattctcaca gacgtcgcag atccataaaa aagataacca
     9301 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     9361 tgcttctacc agcccgttct acccgctgtt tgccgcgctg gacgttaacg ccaaaattca
     9421 tgaaggggag agtgggcgtc ggctgtgggc tgagtgcgtt gagttgggta ttgagtcgcg
     9481 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccccccg ttgttgatgg
     9541 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     9601 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     9661 tccgtgcaag ctgttactca ctacaccagg tatcgatgcc gaaaccggcg aatatagcga
     9721 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     9781 gaagtgcgat ctcaactcca ttctgttctt attaactccg gcggaaagcc acgagaagct
     9841 ggcacaactg gtagagatgt tggcgcagtt tgaacagcat attgaggatg actcgccgct
     9901 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
     9961 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
    10021 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
    10081 tagcgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
    10141 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
    10201 ctggggtggg gcggttcaac gttatttcct tgcactggaa gaaggggtga atttgttgcc
    10261 gggattttcg ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg
    10321 gttgtacggt tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt
    10381 ttttctttgc gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta
    10441 tatttgaaca tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac
    10501 catacggtct gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc
    10561 acgatgccgc cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca
    10621 ctggcgcgaa ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag
    10681 aagtcgaatg cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga
    10741 gtcgggtcgc cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc
    10801 attacgttct taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt
    10861 tcagagatct gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca
    10921 aacatcggat ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc
    10981 agttccgcgc ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac
    11041 agcgcgaatg catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca
    11101 accggaatat gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca
    11161 atatacagct gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg
    11221 ataaagccga tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg
    11281 gcattttgca ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca
    11341 aacgagttaa tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg
    11401 aacagcgtga tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat
    11461 ttgtcggcca caatccccag cagcgcaggc ataaagaccg cggcgatacc cagtgagcta
    11521 taaactgcgc caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg
    11581 gtcgtcagcc aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc
    11641 ttaagattca tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt
    11701 tgaccgtgcg agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg
    11761 caaataattg caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg
    11821 cccctgagca tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg
    11881 gtattcactt tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc
    11941 tgataaacat cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc
    12001 gaaaagactc gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc
    12061 cgcgatgttg ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg
    12121 gtgccggtat caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat
    12181 ttcccctgcg agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc
    12241 agcgcatcgg aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc
    12301 agaatcagcg actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat
    12361 ttgtgcgcac gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata
    12421 cgcagcccgt tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt
    12481 gcgcgacctt cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa
    12541 ggttctttcg aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg
    12601 tcacccatca ataacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgccccg
    12661 gcgatggttt cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat
    12721 tgatcggtgt atttcacgta gtccttagga ccagcgatca ccacctcttt gaagccccag
    12781 atgttctcaa tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtgtctttg
    12841 acccaggctt cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc
    12901 gcaatcaaag ccagcgcgag atattttttc atcattccag gtgcgtgttg tgtttgtttt
    12961 tatgcaagtt gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata
    13021 gccctcgata tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag
    13081 cttgcggtgc tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt
    13141 gtgctgccac tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc
    13201 gccggggtac agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat
    13261 cgttctgctc ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg
    13321 taactgctgt aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa
    13381 gttataccag agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca
    13441 cataggcaca atatctaagt ggaaatggct gaaggtatgg cgaaacgcgg tcagctgcgt
    13501 caggttatcg gcagcaatct gccgttgcgc cagccaatgc cgcaaacttt cttcgtcggc
    13561 aaactgcggg aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa
    13621 tacttcatct tcgtgctgta atagcaaaaa gtagccggtg cgctccggca gcgtctgttt
    13681 cggttttttg cccggataaa gcgcccagct attgttggcg gcggcaatac atccgttttg
    13741 tagcggacag agcgaacatt tcggtttcga gcgcgtacaa atcatcgcac ccaaatccat
    13801 catcgcctga ttaaaccgtt ccacgccaac cgcgggcgtc acctgctcgc tcaaactcca
    13861 taatttattc tcgacctctt ttttccctgg ccagccgctt acagcatagc agcgcgccag
    13921 cacccgtttg acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat
    13981 cgcgcctgcg gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg
    14041 gaatttaccg ccgtgtaagg tcgccacttg ttgtgccgct ttatgcaggt tgcgcgcgcg
    14101 ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag
    14161 atcggtcacc gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac
    14221 ctgagtttgt tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg
    14281 ccagggcagc gttttccgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg
    14341 cgacgcttgc atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg
    14401 gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact
    14461 aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa
    14521 cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt
    14581 tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc
    14641 ggtgatgggc gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga
    14701 agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa
    14761 agatcgccct gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg
    14821 cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc acgatgcggt
    14881 tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc
    14941 tgacccgtgg cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga
    15001 actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc
    15061 ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga
    15121 gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg
    15181 tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag
    15241 aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt
    15301 tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat
    15361 gattttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg
    15421 gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg
    15481 attgtgcgta agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc
    15541 gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg
    15601 ctacgcttat caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc
    15661 gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg
    15721 attttttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg
    15781 acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt
    15841 gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac
    15901 ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg
    15961 aaggattatc aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc
    16021 cgcgtcgaaa aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc
    16081 agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc tgaaagagca gatgaaccgc
    16141 attatcgaaa cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc
    16201 gccgaaggcc agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat
    16261 gaaatgggcg cgaaagcggt gttgaaaagc ggcggtaacc cattacagaa tgtgctggga
    16321 agcctggggg ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc
    16381 cagcagtttg gcaaagatgt ttgtagccgt gttgtgactc tggaagatag ccgcaaagcc
    16441 ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt
    16501 ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt
    16561 gtaaattgtt taacgtcaaa tttcccatac aaagctaagg gataatgcgt agcgttcacg
    16621 taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg
    16681 ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat
    16741 tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga
    16801 aaatctggtt aatgcggtgc cgcaactgaa ggacattgcg aacgttaaag gcgagcaggt
    16861 agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat
    16921 taacaccgac tgcgataaga ccgacggctt cgtcattacc cacggtaccg acacgatgga
    16981 agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg
    17041 cgcaatgcgt ccgtccacgt ctatgagcgc agacggtcca ttcaacctgt ataacgcggt
    17101 agtgaccgca gctgataaag cctccgccaa ccgtggcgtg ctggtagtga tgaatgacac
    17161 cgtgcttgat ggccgtgacg tcaccaaaac caacaccacc gacgtagcga ccttcaagtc
    17221 tgttaactac ggtcctctgg gttacattca caacggtaag attgactacc agcgtacccc
    17281 ggcacgtaag cataccagcg acacgccatt cgatgtctct aagctgaatg aactgccgaa
    17341 agtcggcatt gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga
    17401 tgcgggctat gatggcatcg ttagcgctgg tgtgggtaac ggcaacctgt ataaatctgt
    17461 gttcgacacg ctggcgaccg ccgcgaaaaa cggtactgca gtcgtgcgtt cttcccgcgt
    17521 accgacgggc gctaccactc aggatgccga agtggatgat gcgaaatacg gcttcgtcgc
    17581 ctctggcacg ctgaacccgc aaaaagcgcg cgttctgctg caactggctc tgacgcaaac
    17641 caaagatccg cagcagatcc agcagatctt caatcagtac taatcacctc gccccggtat
    17701 cgtgccgggg ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga
    17761 taatcaccgg aataaattat tccgcgtgag gtttttcggg tgaaaaagca atggattgtt
    17821 ggcacggcac tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact
    17881 gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct
    17941 atcaccttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat
    18001 gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc
    18061 gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga
    18121 acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc cagcgatcgt
    18181 gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat
    18241 ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct
    18301 gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg
    18361 gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa
    18421 aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt
    18481 aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag
    18541 tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag
    18601 acttttctgc aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgcttgaa
    18661 aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt
    18721 ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa
    18781 ctgcggcgca atggggtgat taagtaagtt tgatatagtg cctttgccgg ataatataaa
    18841 aagttatccg gcaaattatt ttttacatta tccctagtag ctgtggaatg aacaaggata
    18901 tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa aatggcagca
    18961 atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca aatagcgcac
    19021 tacccaccgg cggtgttaac agcccaatac aaaggttggc caccatcata ataccgaaat
    19081 gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta aagattagta
    19141 cagccggtgt catgtccata aagatgccga caattaataa gacgatatta atcagcagca
    19201 atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata taaggaatat
    19261 cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt aatacaatcg
    19321 aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt acttcccggt
    19381 aaaccagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct tcagttgcgg
    19441 taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg
    19501 catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac
    19561 ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag atccctggca
    19621 aatatcccgc cataaatagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa
    19681 ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg
    19741 tagaaaatgc cggatcataa ccttgtttgg tctgaatggg attcagtgtg ccaccaacgg
    19801 cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt
    19861 tcacatgacc aagtgaacca ggaacccgac caaccattac ctgggcgagg ttaatcaaac
    19921 gtatcgcaat accgccgctg ttcatcagcg ttccggcaaa gatgaaaaag gggattgcca
    19981 atagggcaaa gttatccagt ccattagcca gtcgctgcga aacagtgatt gccgcgatat
    20041 cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg
    20101 gcacaccaat aaagaccaac acaaaaaaac agccaaacag tgtaatcgcg atataactat
    20161 ccattaactg gcctcctgcg catgttgttt cagttgttga atgctttgat taataaaaca
    20221 caaggcataa aacatcatca ccaatccact taatggcaga atgatatata cgtatcccat
    20281 cggtatttgc atcgcggcag atacctgtga tgttgccagc gttttatcaa ttaatttcag
    20341 gccaccagtc acaatgaccg aaccggcaaa acctaaaata agaacgttaa tgactatact
    20401 aagaagtaat tgcttgcgct tattcagggc gagagcaaat aaatcgatag agagatgtct
    20461 ctgcgcgccg acggtatatg cagcacctaa taaccctacc cagatcatca aaaatcgtgc
    20521 cagctcatcg gttaatgtac ttggctggtt aagtacgtag cggctaaata cttgccagac
    20581 tacgcataca accagagcaa gcatgacggc gacagaaaac gcggcaatga cgcggtctac
    20641 cgcaagtttc aggcggttaa atatcattgt aagcatcctg gaaaatcagg gccagagact
    20701 ggcccggaat attattgagc agcgttgtcg aacttctcta acaaagcagc ttgttttgga
    20761 tcttttttga agtcgtcaaa cagaggctga actgcggcac ggaatggtgc tttatccact
    20821 tttacaattt caccccccat agctttcgcc tgagcacgtg tgtcagcgtc aattttttcc
    20881 cacaattttt gttgataagc ttcggatttc ttcgctgctg tttcgaggat ttgttgctgt
    20941 tctggggtta atttattcca tgttttggtg gagataacca aaaagtcagg aattgaggcg
    21001 tgttcatctt cagaaaaaac tttggcaatt tcaatatgtc gagtctgcac ccaggaaggc
    21061 acgttatttt ctgcgccatc tactacacct tgctgcatcg cagtataaac ttcgccaaat
    21121 gaaattggag tgggtgaacc acccatcagt tcaatcattt tgatggtggt cggacttggc
    21181 tgaacacgaa ttttcagccc tttaagatct tctggcttag taatcggttt tttcgcatag
    21241 aaactgcgtg tacccgctac ataagctgat aaagcaaaaa agcctttgtc ttttgttgag
    21301 tccataattt ctttaccaac ttcaccaaaa acaactttgt tgaagtgagc ctgatcttta
    21361 aataagaacg gcaaattata gatagcataa atattatcaa aagactccag atcactggcg
    21421 gaacctttgg tcatatccag agcgccattt tgcaacaact ccatagtttc tcgcgcatta
    21481 cccatctggc tgctgggata aatacggatc accatattac cttttgacag ctgttttacc
    21541 tctttcgcca gctcttcaaa actctgatgt acaacatgac ttctttccag gttgtgtgct
    21601 aactttaacg tcactttttc tgcggcgttt acgccagtgc taaccagagc cagcaaggat
    21661 gcgcagacta atgagcgggt aaatgacctc attttcataa aaaaactccc tgttgaagga
    21721 tggaaattgg ttgatatcta attgtgctta tcagtgcttt gtgcgacaaa ttggttaacc
    21781 tgtctgacaa cttgtctatg tttatcacaa cttgaaataa agtcagaaat taaatattaa
    21841 atttgtgcgc caggttgtac atgtttataa tca
//
LOCUS       DAMZII010000080        21505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZII010000080 DAMZII010000000
VERSION     DAMZII010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.080"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            8..148
                     /locus_tag="POA77_002941"
     CDS             8..148
                     /locus_tag="POA77_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDI6366339.1"
                     /translation="MATVQKDGRRVEFTATSVSDLKKYIAELEVQTGMTQRRRGPAGF
                     YV"
     gene            145..1746
                     /locus_tag="POA77_002942"
     CDS             145..1746
                     /locus_tag="POA77_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6366340.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            1727..3046
                     /locus_tag="POA77_002943"
     CDS             1727..3046
                     /locus_tag="POA77_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDI6366341.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            3056..3388
                     /locus_tag="POA77_002944"
     CDS             3056..3388
                     /locus_tag="POA77_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDI6366342.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMPDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            3444..4469
                     /locus_tag="POA77_002945"
     CDS             3444..4469
                     /locus_tag="POA77_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDI6366343.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            4511..4906
                     /locus_tag="POA77_002946"
     CDS             4511..4906
                     /locus_tag="POA77_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDI6366344.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            4918..5271
                     /locus_tag="POA77_002947"
     CDS             4918..5271
                     /locus_tag="POA77_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDI6366345.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            5283..5861
                     /locus_tag="POA77_002948"
     CDS             5283..5861
                     /locus_tag="POA77_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6366346.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            5858..6253
                     /locus_tag="POA77_002949"
     CDS             5858..6253
                     /locus_tag="POA77_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6366347.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPAPSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            6261..7001
                     /locus_tag="POA77_002950"
     CDS             6261..7001
                     /locus_tag="POA77_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6366348.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            7017..7439
                     /locus_tag="POA77_002951"
     CDS             7017..7439
                     /locus_tag="POA77_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDI6366349.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSMVS"
     gene            7421..7855
                     /locus_tag="POA77_002952"
     CDS             7421..7855
                     /locus_tag="POA77_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDI6366350.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            7848..10427
                     /locus_tag="POA77_002953"
     CDS             7848..10427
                     /locus_tag="POA77_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6366351.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLMLQTELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAASGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            10424..10753
                     /locus_tag="POA77_002954"
     CDS             10424..10753
                     /locus_tag="POA77_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6366352.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            10753..11451
                     /locus_tag="POA77_002955"
     CDS             10753..11451
                     /locus_tag="POA77_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDI6366353.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSMRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            11457..12200
                     /locus_tag="POA77_002956"
     CDS             11457..12200
                     /locus_tag="POA77_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6366354.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            12137..12769
                     /locus_tag="POA77_002957"
     CDS             12137..12769
                     /locus_tag="POA77_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDI6366355.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGTGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            12830..16327
                     /locus_tag="POA77_002958"
     CDS             12830..16327
                     /locus_tag="POA77_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDI6366356.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCM
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLMFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGEPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            16398..16997
                     /locus_tag="POA77_002959"
     CDS             16398..16997
                     /locus_tag="POA77_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HDI6366357.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            17062..20460
                     /locus_tag="POA77_002960"
     CDS             17062..20460
                     /locus_tag="POA77_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDI6366358.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEDDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGISTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            20460..21041
                     /locus_tag="POA77_002961"
     CDS             20460..21041
                     /locus_tag="POA77_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI6366359.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALISELGPLPENVTWLSPG
                     GEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEEI
                     SLLEAWKKYRVLLNRVDTSTAPDIEWPTGPIIE"
     gene            complement(21115..>21505)
                     /locus_tag="POA77_002962"
     CDS             complement(21115..>21505)
                     /locus_tag="POA77_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6366360.1"
                     /translation="LDIDNFKSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGG
                     EEFGIIIKGNTQKLLLSIAERIRKNIEEQCSKKLLSHGPEKITVSIGCFTSKENNLSP
                     SEMLVNADKALYQAKRTGKNKVITHSK"
BASE COUNT         5026 a         5410 c         6614 g         4455 t
ORIGIN      
        1 taaacgggtg gcaacagtac agaaagacgg acggcgagtg gagtttacgg ccacttccgt
       61 gtctgacctg aaaaaatata ttgcagagct ggaagtgcag accggcatga cacagcgacg
      121 caggggacct gcaggatttt atgtatgaaa acgcccacca ttcccaccct tctggggccg
      181 gacggcatga catcgctgcg tgaatatgcc ggttatcatg gcggtggcag cggatttggt
      241 gggcagttgc gggcgtggaa tccaccgagt gaaagtgtgg atgcagccct gctgcccaac
      301 tttacccgtg gcaatgcccg cgcagacgat ctggtgcgca ataacggcta tgccgccaac
      361 gccatccagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca
      421 agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa
      481 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc
      541 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc
      601 gttcaggcca cctgggatac cagctcgtcg cggctgttcc ggacacagtt ccggatggtc
      661 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt
      721 gtgcagatta atgacagcgg cgcggcgctg ggatattacg tcagcgagga cgggtatcct
      781 ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcctcg
      841 ttcattcacg tttttgaacc cgtggaggac gggcagaccc gcggtgcaaa tgtgttttac
      901 agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct gcagagcgcc
      961 attgtgaagg cgatgtatgc cgccaccatt gagagtgagc tggatacgca gtcagcgatg
     1021 gattttattc tgggcgcgaa cagtcaggag cagcgggaaa ggctgactgg ctggattggt
     1081 gaaattgccg cgtattacgc cgcagcaccg gtccggctgg gaggcgcaaa agtgccgcac
     1141 ctgatgccgg gggactcact gaacctgcag acggctcagg acacggataa cggctactcc
     1201 gtgtttgagc agtcactgtt gcggtatatt gctgccgggc tgggtgtctc gtatgagcag
     1261 ctttcccgga attacgccca gatgagctac tccacggcac gggccagtgc gaacgagtcg
     1321 tgggcgtact ttatggggcg gcgaaaattc gtcgcatccc gtcaggcgag ccagatgttt
     1381 ctgtgctggc tggaagaggc catcgttcgc cgcgtggtga cgttaccttc aaaagcgcgc
     1441 ttcagttttc aggaagcccg cagtgcctgg gggaactgcg actggatagg ctccggtcgt
     1501 atggccatcg atggtctgaa agaagttcag gaagcggtga tgctgataga agccggactg
     1561 agcacctacg agaaagagtg cgcgaaacgc ggtgacgact atcaggaaat ttttgcccag
     1621 caggtccgtg aaacgatgga gcgccgcgca gccggtctta aaccgcccgc ctgggcggct
     1681 gcggcatttg aatccggact gcgacaatca acagaggagg agaagagtga cagcagagct
     1741 gcgtaatctc ccgcatattg ccagtatggc ctttaatgag ccgctgatgc ttgaacccgc
     1801 ctatgcgcgg gttttctttt gtgcgcttgc aggccagctt gggatcagcc gcctgacgga
     1861 tgcggtgtcc ggcgacagcc tgactgccgg agaggcaccc gcggcgctgg cgttatccgt
     1921 tgatgatgac ggaccacgac aggcccgcag ttatcaggtc atgaacggca tcgccgtgct
     1981 gccggtgtcc ggcacgctgg tcagccggac gcgggcgctg cagccgtatt cggggatgac
     2041 cggttacaac ggcattatcg cccgtctgca acaggctgcc agcgatccga tggtggacgg
     2101 cattctgctg gatatggaca caccgggcgg gatggtggcg ggagcatttg actgtgctga
     2161 catcatcgcc cgtgtgcgag acataaaacc ggtatgggcg ctggccaacg acatgaactg
     2221 cagtgcaggt cagctgcttg ccagcgccgc ctcccggcgt ctggtcacgc agaccgcccg
     2281 gacaggctcc atcggcgtca tgatggctca cagtaattac ggcgctgcgc tggagaaaca
     2341 gggcgtggaa atcacgctga tttacagcgg cagccataag gtggatggca acccctacag
     2401 ccatctaccg ggtgatgtcc gggagacact gcagtcccgg atggatgcaa cccgccggat
     2461 gtttgcgcag aaggtgtcgg catataccgg cctgtccgtg caggctgtgc tggataccga
     2521 ggctgcagtg tacagcggtc aggaggccat tgatgccgga ctggctgatg aacttgtcaa
     2581 cagcaccgat gcgatcaccg ttatgcgtga tgcactggat gcacgtaaat cccgtctctc
     2641 aggagggcga atgaccaaag agactcaatc aacaactgtt tcagccactg cttcgcaggc
     2701 tgacgttact gacgtggtgc aagcgacgga gggcgaaaac gccagcgcgg cgcagccgga
     2761 cgtgaacgcg cagatcaccg ctgcggttgc ggcagaaaac agccgcatta tggggatcct
     2821 caactgtgag gaggctcacg gacgcgaaga acaggcacgt gtgctggccg aaacccccgg
     2881 tatgaccgtg gaaacggccc gccgcattct ggcagctgca ccacagagtg cacaggcgcg
     2941 cagtgacact gcgctggatc gtctgatgca gggggcaccg gcaccactgg ctgcaggtaa
     3001 cccggcatct gatgccgtta acgatttgct gaacacacca gtgtaaggga tgtttatgac
     3061 gagcaaagaa acctttaccc attaccagcc gctgggcaac agtgacccgg cacatacggc
     3121 aaccgcgccc ggcggattga gtgcgaaagc gcctgcaatg accccgctga tgccggatac
     3181 ctccacccgt aagctggttg cgtgggatgg caccaccgac ggtactgccg ttggcattct
     3241 ggcggttgat gctgaccaga ccagcaccac gctgacgttc tacaagtccg gcacgttccg
     3301 ttatgaggat gtgctctggc cggaggctgc cagcgacgag acgaaaaaac ggaccgcgtt
     3361 tgccggaacg gcaatcagca tcgtttaacc ttacccttca tcactaaagg ccgcctgtgc
     3421 ggcttttttt acgggatttt tttatgtcga tgtacacaac cgcccagctg ctggcggcaa
     3481 atgagcagaa atttaagttt gatccgctgt ttctgcgtct ctttttccgt gagagctatc
     3541 ccttcaccac ggagaaagtc tatctctcac aaattccggg actggtaaac atggcgctgt
     3601 acgtttcgcc gattgtttcc ggtgaggtta tccgttcccg tggcggctcc acctctgaat
     3661 ttacgccggg atatgtcaaa cccaagcatg aggtgaatcc gcagatgacc ctgcgtcgcc
     3721 tgccggatga agatccgcag aatctggcgg acccggctta ccgccgccgt cgcatcatca
     3781 tgcagaacat gcgagacgaa gagctggcca ttgctcaggt cgaagagatg caggcagttt
     3841 ctgctgtgct taagggcaaa tacaccatga ccggtgaagc cttcgatccg gttgaggtgg
     3901 atatgggccg cagtgcggcg aacaacatca cacagtccgg tggtacggag tggagcaagc
     3961 gtgacaagtc cacgtatgac ccgaccgacg atatcgaagc ctatgcgctg aacgccagcg
     4021 gcgtggtgaa tatcatcgtg tttgatccga aaggctgggc gctgttccgt tccttcaaag
     4081 ccgtcaagga gaagctggat acccgtcgcg gctctcattc cgagctggag acagcggtaa
     4141 aagacctggg cgaagcggtg tcctataagg ggatgtatgg cgatacggcg atcgtcgtgt
     4201 attccggaca gtacgtggaa aacgacgtca aaaagaactt cctgccggac aacacgatgg
     4261 tgctggggaa cactcaggca cgcggtctgc gcacctatgg ctgcattcag gatgcggacg
     4321 cacagcgcga aggtattaac gcctctgccc gctacccgaa aaactgggtg accaccggcg
     4381 atccggcgcg tgagttcacc atgattcagt cagcaccgct gatgctgctg gctgatcctg
     4441 atgcgttcgt gtccgtacaa ctggcgtaat catggccctt cggggccatt ttctctctgt
     4501 ggaggagtcc atgacgaaag atgaactgat tgcccgtctt caggtgctgg gtgagcaact
     4561 gaaccgtgat gtcagcctga cggggacgaa agaagaactg gtgctccgtg tggcagagct
     4621 ggaagaggag cttgatgaca cggatgacgc tgccggtcag gacacatctg tcagcccgga
     4681 aaatgcgctg accggacatg aaaatgaggt ggtatcagcg cagccggata ccgtgattga
     4741 tacggctgct ctggtcacgg tcgtggcact ggtgacgctg catactgatg cacttcacgc
     4801 cacgcgggat gagcctgtgg catttgtgct gccgggaacg gcgtttcgtg tctctgccgg
     4861 tgtggcagcc gaaatgacag aacatggcct ggccagaatg caataacggg aggcgctgtg
     4921 gctgattccg ataacctgtt cgatgctgcc attgcccgcg ccgatgaaac gatacgcggg
     4981 tacatgggaa cgtcagccac catgacatcc ggtgagctgt ccggtgctgt gatacgtggt
     5041 gtttttgatg accctgaaaa tatcagctat gccggacagg gggtgcgcgt tgaaggctcc
     5101 agcccgtccc tgtttgtccg gactgatgat gtgcggcagc tgcggcgtgg agacacactg
     5161 accatcggcg aggaaaactt ctgggtggac cggatttcgc cggatgatgg cggaagctgt
     5221 catctctggc ttgggcgggg cgtaccgcct gccgttaacc gtcgccgctg aaggggggat
     5281 gtatggccat aaaaggtctt gagcaggccg ttgaaaacct cagtcgtatc agcaaaacgg
     5341 cggtgcccgg tgcgtcagca atggccatta accgcgttgc gtcatccgcg atatcgcagt
     5401 ctgcgtcaca ggttgcccgt gagacaaggg tacgccggaa actggtaaag gaaagggcca
     5461 ggctgaaaag ggccacggtt aaaaatccgc aggccagaat cagggttaac cggggggatt
     5521 tgcccgtaat caagctgggt aacgcgcggg ttgtcctgtc ccgacgcagg cgtcgtaaaa
     5581 aggggcagcg ttcatccctg aaaggtggcg gcagcgtgct tgtggtggga aaccgtcgta
     5641 ttcccggcgc gtttattcag caactgaaaa atggccgctg gcatgtcatg cagcgtgtgg
     5701 ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggtg ccgctgacca
     5761 cggcgtttaa acagaatatt gagcggatac ggcgtgagcg tcttccgaaa gagctgggct
     5821 atgcgctgca gcatcaactg aggatggtaa taaagcgatg aaacatactg aactccgtgc
     5881 agccgtactg gatgcactgg agaagcatga caccggggcg acgttttttg atggtcgccc
     5941 cgctgttttt gatgaggcgg attttccggc agttgccgtt tatctcaccg gcgctgaata
     6001 cacgggcgaa gagctggaca gcgatacctg gcaggcggag ctgcatatcg aagttttcct
     6061 gcctgctcag gtgccggatt cagagctgga tgcgtggatg gagtcccgga tttatccggt
     6121 gatgagcgat atcccggcac cgtcagattt gatcaccagt atggtggcca gtggctatga
     6181 ctaccggcgc gacgatgatg cgggcctgtg gagttctgcc gatctgactt atgtcattac
     6241 ctatgaaatg tgaggacgat atgcctgtac caaatccagc aataccggtg aaaggtgccg
     6301 gaaccaccct gtgggtttat aacgggagcg gcgaccctta tgcgaacccg ctttcagacg
     6361 ttgactggtc gcgtctggct aaagttaaag acctgacgcc cggcgaactg accgctgagt
     6421 cctatgacga cagttatctc gatgatgaag atgcggactg gaccgcgacc gggcaggggc
     6481 agaaatctgc cggagatacc agcttcacgc tggcgtggat gcccggagag caggggcagc
     6541 aggcgctgct ggcgtggttt aatgaagggg atacccgtgc ctataaaatc cgcttcccga
     6601 acggcacggt cgatgtgttc cgtggctggg tcagcagtat cggtaaggcg gtgacggcga
     6661 aggaagtgat cacccgtacg gtgaaggtca ccaatgtggg ccgtccgtca atggcagaag
     6721 atcgcagtac ggtgacggcg gcaaccggca tgaccgtgac gcctgccagc acttcggtgg
     6781 tgaaagggca gagcaccacg ctgaccgtgg cattccagcc ggaaggcgca accgacaaga
     6841 gcttccgtgc ggtgtctgcg gataaaacaa aagccaccgt gtcggtcagt ggtatgacca
     6901 tcaccgtgaa aggtgttgct gcaggcaagg tcaacattcc ggttgtatcc ggtaatggtg
     6961 aacttgctgt ggttgcagaa atcaccgtca ccgacagtta atccggagag tcagcgatgt
     7021 tcctgaaaac cgaatcattt gaatataacg gtgtgagcgt cacgctttct gaactgtcag
     7081 ccctgcagcg aattgagcat ctcgccctgc tgaaacgaca ggcagaacag gcgggatcca
     7141 gtctcaatcg acaggtgagc gtggaagatc tcgtcagaac cggtgctttt ctggtggcga
     7201 tgtccctgtg gcatagccat ccgcagaaga caaagatgcc gtccatgaat gaagccgtta
     7261 aacaaattga gcaggaagtg cttaccacct ggcccacaga ggcaatttct catgctgaaa
     7321 acgtggtgta ccggctgtcc ggtatgtatg agtttgtggt gaatgatgct cctgaacaga
     7381 cagaggacgc cgggcctgca gagcctgttt ctgcgggaaa gtgttcgatg gtgagctgag
     7441 ttttgccctg aaactggcgc gagagatggg gcgacccgac tggcgtgcca tgcttgccgg
     7501 gatgtcatcc acggagtatg ccgactggca ccgcttttac agtacccatt attttcatga
     7561 tgttctgctg gatatgcact tttccgggct gacgtacacc gtgctcagcc tgtttttcag
     7621 cgatccggat atgcatccgc tggatttcag tctgctgaac cggcgtgagg ctgacgaaga
     7681 gcctgaagat gatgtgctga tgcagaaagc ggcagggctt gccggaggcg tccgctttgg
     7741 cccggacggg aatgaagtta tccccgcttc cccggatgtg gcggacatga cggaggatga
     7801 cgtaatgctg atgacagtat cagaagggat cgcaggagga gtccggtatg gctgaaccgg
     7861 taggcgatct ggtcgttgat ttaagtctgg atgcggccag atttgacgag cagatggcca
     7921 gagtcaggcg tcatttttcc ggtacggaaa ctgatgcgaa aaaaacagcg gcagtcgttg
     7981 aacagtcgct gagccgacag gcgctggctg cacagaaagc ggggatttcc gtcgggcagt
     8041 ataaagccgc catgcgtatg ctgcctgcac agttcaccga cgtggccacg cagcttgcag
     8101 gcgggcaaag cccgtggctg atcctgctgc aacagggggg tcaggttaag gactccttcg
     8161 gcgggatgat ccccatgttc agggggcttg ccggtgcgat caccctgccg atggtggggg
     8221 ccacctcgct ggcggtggcg accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa
     8281 ccctgtccga tttcaacaaa acgctggtcc tttccggcaa tcaggcggga ctgacggcag
     8341 atcgtatgct ggtcctgtcc agagccgggc aggcggcagg gctgacgttt aaccagacca
     8401 gcgagtcact gacggcgctg gtgaatgccg gtgtgcgtgg tggtgagcag tttgaggcga
     8461 tcagccagag tgtggcgcgt ttctcctctg catccggcgt ggaggtggac aaggtcgctg
     8521 aagccttcgg gaagctgacc acagacccga cgtcgggact gacagcgatg gcacgtcagt
     8581 tccataacgt gacggcggag cagattgcgt atgttgctca gttgcagcgt tccggagatg
     8641 aagccggggc attgcaggcg gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc
     8701 gccgcctgaa agagaacatg ggcacgctgg agacctgggc agacaggaca gcacgggcat
     8761 tcaaatccat gtgggattcg gtgctggata ttggtcgccc ggacactgcc cagggaatgc
     8821 tggagaaagc agaaaaggct tttgatgagg cggacaaaaa atggcagtgg tatcagagcc
     8881 ggagccaccg gcgcggtaaa acctcagcat ttcttgccaa tctccgggga gcatgggagg
     8941 acagagcgaa tgcgcaactt gggctttcag ccgccacgtt gcaggccgat cttgaaaagg
     9001 ccagagagat ggcagcaaag gactgggccg agtctgaggc atcacggctg aaatataccg
     9061 aagaggcgca gaaggcttac gaacggctgc agacgccgct ggagaaatat accgcccgtc
     9121 aggaagaact gaacaaggca ctgaaagacg ggaaaatcct gcaggcggat tacaacacgc
     9181 tgatggcggc ggcgaaaaag gattatgaag cgacgctgaa aaagccgaaa cagtccggcg
     9241 tgaaggtgtc tgcgggcgat cgtcaggaag acagtgctca tgctgccctg ctgatgcttc
     9301 agacagaact ccggacgctg gagaagcatg ccggagcgaa tgagaaaatc agccagcagc
     9361 gccgggattt gtggaaggca gaaagtcagt tcgcggtact ggaggaggcg gcgcaacgtc
     9421 gtcagttgtc tgcacaggag aaatccctgc tggcgcataa agatgagacg ctggagtaca
     9481 aacgccagct ggctgcactt ggcgacaagg ttacgtatca ggagcgcctg aacgcgctgg
     9541 cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggccgcc attgatgcga
     9601 aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag cgcctgaagg
     9661 aacagtatgg cgataatccg ctggcgctga ataacgtcat gtcagagcag aaaaagacct
     9721 gggcggctga agaccagctt cgcgggagct ggatggcagg cctgaagtcc ggctggagtg
     9781 agtgggaaga gagcgccacg gacagtatgt cgcaggttaa aagtgcagcc acgcagacct
     9841 ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgagcag aactggcgca
     9901 gcttcacccg ttccgtgctg tccatgatga cagaaattct gcttaagcag gcaatggtgg
     9961 ggattgtcgg gagtatcggc agcgccattg gcggtgctgc cagtggtgga gcatccgcgt
    10021 caggcggtac agccattcag gccgctgcgg cgaaattcca ttttgcgacc gggggattta
    10081 cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgaa tttgtcttca
    10141 cgaaggaggc aaccagccgg attggtgtcg gcaacctgta ccgcctgatg cggggctatg
    10201 cggaaggtgg ttatgtgggc ggtgccggaa gtccggcgca gatgcggcgg gctgaaggca
    10261 ttaattttaa tcagaacaat cacgtggtga ttcagaacga cggtacgaat ggtctgccag
    10321 gtccacagat gatgaaggca gtgtatgaca tggcccgcaa gggtgcccgt gatgaaattc
    10381 agacacagat gcgtgatggt ggcctgttct ccggaggtgg acgatgaaaa ccttccgctg
    10441 gaaagtgaaa cccggtatgg atgtggcttc ggccccttct gtaagaaagg tgcgctttgg
    10501 tgatggctat tcccagcgag cgcctgccgg gctgaatgcc aacctgaaaa cgtacagcgt
    10561 gacgctttct gtcccccgtg aggaggccac ggtactggag tcgtttctgg aagagcacgg
    10621 gggctggaaa gcctttctgt ggacgccgcc ttatgagtgg cggcagataa aggtgacctg
    10681 cgcaaaatgg tcgtcgcggg tcagtatgct gcgtgttgag ttcagcgcag agtttgaaca
    10741 ggtggtgaac tgatgcagga tatccggcag gaaacactga atgaatgcac ccgtgcggag
    10801 cagtcggcca gcgtggtgct ctgggaaatc gacctgacag aggtcggtgg agaacgttat
    10861 tttttctgta atgagcagaa cgaaaaaggt gagccggtca cctggcaggg gcgacagtat
    10921 cagccgtatc ccattcaggg gagcggtttt gaactgaatg gcaaaggcac cagtatgcgc
    10981 cccacgctga cggtttctaa cctgtacggt atggtcaccg ggatggcgga agatatgcag
    11041 agtctggtcg gcggaacggt ggtccggcgt aaggtttacg cccgttttct ggatgcggtg
    11101 aacttcgtca acggaaacag ttacgccgat ccggagcagg aggtgatcag ccgctggcgc
    11161 attgagcagt gcagcgaact gagcgcggtg agtgcctcct ttgtactgtc cacgccgacg
    11221 gaaacggatg gcgctgtttt tccgggacgt atcatgctgg ccaacacctg cacctggacc
    11281 tatcgcggtg acgagtgcgg ttatagcggt ccggctgtcg cggatgaata tgaccagcca
    11341 acgtccgata tcacgaagga taaatgcagc aaatgcctga gcggttgtaa gttccgcaat
    11401 aacgtcggca actttggcgg cttcctttcc attaacaaac tttcgcagta aatcccatga
    11461 cacagacaga atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg gagtcgtgcg
    11521 gcttcgtggt aagcacgccg gagggggaaa gatattttcc ctgcgtgaat atctccggtg
    11581 agccggaggc gtatttccgt atgtcgccgg aagactggct gcaggcagaa atgcagggtg
    11641 agattgtggc gctggtccac agccaccccg gtggtctgcc ctggctgagt gaggccgacc
    11701 ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg acgattcata
    11761 agttccgctg tgtgccgcat ctcaccgggc ggcgctttga gcacggtgtg acggactgtt
    11821 acacactgtt ccgggatgct tatcatctgg cggggattga gatgccggac ttttatcgtg
    11881 aggatgactg gtggcgtaac ggccagaatc tctatctgga taatctggag gcgacggggc
    11941 tgtatcaggt gccgttgtca gcggcacagc cgggcgatgt gctgctgtgc tgttttggtt
    12001 catcagtgcc gaatcacgcc gcaatttact gcggcgacgg cgagctgctg caccatattc
    12061 ctgaacaact gagcaaacga gagaggtaca ccgacaaatg gcagcgacgc acacactccc
    12121 tctggcgtca ccgggcatgg cgcgcatctg cctttacggg gatttacaac gatttggtcg
    12181 ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgggcactgg ccacacagct
    12241 cccggcgttt cgtcagaaac tgagcgacgg ctggtatcag gtacggattg ccgggcggga
    12301 cgtcagcacg tccgggttaa cggcgcagtt acatgagact ctgcctgatg gcgctgtgat
    12361 tcatattgtt cccagagtcg ccggggccaa gtcaggtggc gtattccaga ttgtcctggg
    12421 ggctgccgcc attgccggat cattctttac cgccggagcc acccttgcag catggggggc
    12481 agccattggg accggtggta tgaccggcat cctgttttct ctcggtgcca gtatggtgct
    12541 cggtggtgtg gcgcagatgc tggcaccgaa agccagaact ccccgtacac agacaacgga
    12601 taacggtaag cagaacacct atttctcctc actggataac atggttgccc agggcaatgt
    12661 tctgcctgtt ctgtacgggg aaatgcgcgt ggggtctcgc gtggtttctc aggagatcag
    12721 cacggcagac gaaggggacg gtggtcaggt tgtggtgatt ggtcgctgat gcaaaatgtt
    12781 ttatgtgaaa ccgcctgcgg gcggttttgt catttatgga gcgtgaggaa tgggtaaagg
    12841 cagcagtaag gggcataccc cgcgcgaagc gaaggacaac ctgaagtcca cgcagctgct
    12901 gagtgtgatc gatgccatca gcgaagggcc ggttgaaggt ccggtggatg gattaaaaag
    12961 cgtgctgctg aacagtacgc cggtgctgga cactgagggg aataccaaca tctccggtgt
    13021 cacggtggtg ttccgtgccg gtgagcagga gcagacaccg ccggaggggt ttgaatcctc
    13081 cggctccgag acggtgctgg gtacggaagt gaaatatgac acgccgatca cccgcaccat
    13141 tacgtctgca aacatcgacc gtctgcgctt taccttcggt gtgcaggcac tggtggaaac
    13201 cacctcaaag ggggaccgga atccgtcgga agtccgcctg ctggttcaga tacagcgtaa
    13261 cggtggctgg gtgacggaaa aagacatcac cattaagggc aaaaccacct cgcagtatct
    13321 ggcctcggtg gtggtggata acctgccgcc gcgcccgttt aatatccgga tgcgcaggat
    13381 gacgccggac agcaccacag accagctgca gaacaaaacg ctctggtcgt catacaccga
    13441 aatcatcgat gtgaaacagt gctacccgaa cacggcactg gtcggcgtgc aggtggattc
    13501 ggagcagttc ggcagccagc aggtgagccg taattatcat ctgcgcgggc gcattctgca
    13561 ggtgccgtcg aactataacc cgcagacgcg gcaatacagc ggtatctggg acggaacgtt
    13621 taagccagca tacagcaaca acatggcctg gtgtatgtgg gatatgctga cccatccgcg
    13681 ctacggcatg gggaaacgtc ttggtgcagc ggatgtagat aaatgggcgc tgtatgtcat
    13741 cggccagtac tgcgaccagt cggtgccgga cggctttggc ggcacggagc cgcgcatcac
    13801 ctgtaatgcg tacctgacca cacagcgcaa ggcgtgggat gtgctcagtg atttctgctc
    13861 ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg atgttcgtgc aggaccgacc
    13921 atcagataag gtgtggacct ataaccgcag taatgtggtg atgccggatg atggtgcgcc
    13981 gttccgctac agcttcagcg ccctgaagga ccgccataat gccgttgagg tgaactggat
    14041 tgacccgaac aacggctggg agacggcgac agagcttgtt gaagatactc aggccattgc
    14101 ccgttacggt cgtaacgtca cgaagatgga tgcctttggc tgtaccagcc gggggcaggc
    14161 gcaccgcgcc gggctgtggc tgattaaaac ggaactgctg gaaacgcaga ccgtggactt
    14221 cagcgtgggt gcggaagggc ttcgccatgt accgggggat gtcattgaaa tctgcgatga
    14281 tgactatgcg ggtatcagca tcggcgggcg cgtgctggcg gtgaacagcc agacccggac
    14341 gctgacgctc gaccgtgaaa tcacgctgcc atcctccggt accacgctga taagcctggt
    14401 tgacggaagt ggcaatccgg tcagcgtgga ggtccagtcc gtcaccgacg gcgtgaaggt
    14461 aaaagtgagc cgtgttcctg acggcgttgc cggatacagc gtgtgggggc tgaagctgcc
    14521 gacgctgcgc cagcgcctgt tccgctgcgt gagtatccgt gagaacgacg acggcacgta
    14581 tgccatcacc gccgtgcagc atgtacccga aaaagaagcc atcgtggata acggggcgca
    14641 ctttgacggc gacctgagcg gcacggtgaa tggcgtcacg ccgcccgcgg tgcagcacct
    14701 gactgccgaa gtcaccgcag acagcgggga atatcaggtg ctggcgcgct gggacacgcc
    14761 gaaggtggtg aagggggtga gcttcctgct tcgcctgacc gtggcagcgg acgatggcag
    14821 tgagcggctg gtcagtacgg ccaggacgac ggaaaccaca taccgcttca cgcaactggc
    14881 gctggggaac tacaggctga ctgtccgggc ggtaaatgcg tggggacagc agggcgaacc
    14941 ggcatcggta tcgttccgga ttgccgcacc ggcagcgccg tctcggattg agctgacacc
    15001 gggctatttt cagataaccg ccacgccgca tcttgcggtt tatgatccga cggtacaatt
    15061 tgagttctgg ttctcggaaa cgcggattac cgatatcagg caggttgaaa ccacagcccg
    15121 ctaccttggc acggggctgt actggatagc cgccagtatc aatatcaaac cgggccatga
    15181 ttattacttt tatatccgca gtgtgaacac cgttggcaaa tcggcattcg tggaggctgt
    15241 cggtcagccg agtgatgatg catcaggcta tctggatttt ttcaaaggcg agatagggaa
    15301 aacccatctg gctcaggagc tgtggacgca gattgataac ggtcagcttg cgcctgacct
    15361 gactgaaatc aggacgtcca taacggatgt cagcaatgaa ataacacaga ccgtcaataa
    15421 gaaactggaa gaccagagtg cagcgatcca gcagatacag aaggttcagg ttgatacaaa
    15481 taataacctg aacagcatgt gggcagtgaa gctgcagcag atgcaggacg gacgccttta
    15541 tattgcgggt atcggtgccg gtattgagaa cacccctgac ggcatgcaga gtcaggtgct
    15601 gctggcagca gacaggattg cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt
    15661 tgttggtcag ggcgatcaga tattcatgaa tgaagtgttc ctgaaacgcc tgacggcccc
    15721 caccattacc agcggcggta atcctccggc attttccctg acatcagacg ggagactgac
    15781 ggcgaaaaat gcggatatca gtggcagtgt gaatgcgaac tcaggaacgc tcaacaatgt
    15841 cacgattaac cagaactgta cgattaaggg catgctggag gcgacccagg tcagaggaga
    15901 tttcgttaaa gctgtatcaa aagccttccc gaaaaaagtc ggtacgtggg gtaacacgga
    15961 aacaccaaac ggtacggtta cagtcaccat cagcgatgat cataactttg accgccagat
    16021 tattattccg cccattattt ttaacggtat agcgtatgac gatccgggga gcggaaataa
    16081 cccaggaggc acgcgataca cgggttatgg ttttgaagtt cgcaaaaacg gcgtattaat
    16141 cgcatccaga gaaactaaag gggccattcc cggtagttac agtgcagtta ttgatatgcc
    16201 gagtggcagg ggaagcgtca ctctggagtt taagattttc cagaaaggca atcagggggc
    16261 aggcaatatc accgactgta cggtgattgt gaccaaaaaa gccgcttccg gcatcagtat
    16321 tcgttgaaat atttataacc ccaataaagg gcgtcaggaa tgacgccttt tttattgcag
    16381 aaaagcgaga ggtaattatg cgtaaagttt gtgcagcaat tttgtccgca gccatttgtc
    16441 tggccgtatc cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg agcgcggggt
    16501 atcttcatgc ctcgacgaac gtccccggca gcgatgatct gaacgggatt aacgtgaaat
    16561 accgttatga gtttacggac acactggggc tggtgacgtc attcagctat gcaggagaca
    16621 agaatcgcca gctgacccgt tacagcgata cccgctggca tgaagattcc gttcgtaacc
    16681 gctggttcag cgtaatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
    16741 cgatggcggg tgtagcttac agccgtgtgt cgactttctc cggggattat cttcgcgtaa
    16801 ctgacaacaa ggggaaaaag cacgatgtgc tgaccggaag tgatgacggt cgccacagca
    16861 acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg
    16921 atattgctta tgaaggctcc ggcagtggtg actggcgcac tgacggtttc atcgtgggtg
    16981 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
    17041 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca
    17101 ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
    17161 gtggtgaaca cgctggcctc agaaaatccg gatgaagccg ggcgttacag catggacgtt
    17221 gagtacgggc agtacagcgt tattctgttg gtggagggat tcccgccgtc acatgccggg
    17281 accatcaccg tgtatgaaga ttcccgaccc ggtacgctga atgattttct cggtgccatg
    17341 acggaggatg atgcccgtcc tgaggcactg cgccgttttg aactgatggt ggaagaggtg
    17401 gcgcgtaacg cgtccgcggt ggcacagaac acggcagccg cgaagaagtc agccagcgat
    17461 gccagcacat cagcccgtga ggcggcaacc catgcgactg atgctgcagg ctcagcacgc
    17521 gcagccagca cgtcagccgg acaggccgcg tcgtcggctc agtcagcatc ttccagcgca
    17581 ggaacggcat caacaaaggc cagtgaagca tcgaaaagtg ctgctgctgc agagtcctca
    17641 aaaagcgcgg cagctaccag tgccggtgcg gcgaaaacgt cagaaacgaa tgcggcagcg
    17701 tcacaacaat cagcagccac ttctgcatcc gccgcgacca cgaaggcgtc agaagcagcc
    17761 acctcagccc gggatgcggc ggcctcaaaa gaggcagcga aatcatcaga aacgaacgca
    17821 tcatcaagcg ccagtagtgc cgcttcctcg gcaacggcgg caggcaattc cgcgaaggcg
    17881 gcaaagacgt ctgagacaaa cgccaggtct tctgaaacgg cagcgggaca gagcgcctcg
    17941 gctgcggcag gctcaaaaac agcggctgcg tcgtctgcca gtgccgcgtc aacaagtgcc
    18001 gggcaggcct cagccagtgc caccgccgcc ggaaaatcgg cagaaagcgc cgcatcgtct
    18061 gcttcaacag ccacaacgaa ggctggcgaa gccactgaac aggccagcgc agcagcaagg
    18121 tctgcttccg cagcgaagac atccgagacg aacgcgaaag cgtcggaaac cagcgcagaa
    18181 tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc atcatcggcg
    18241 tctgcttcaa aagatgaggc gaccagacaa gcgtccgcag cgaagggcag cgccacgacg
    18301 gcatccacga aggcgacaga ggcagctggc agtgcgacgg cggcagcaca gagcaaaagt
    18361 acggcggaat ccgcggcaac gcgcgccgag acagcggcaa aacgggcaga ggatattgca
    18421 tccgccgtgg cgcttgagga tgcaagtacg acgaaaaagg ggatagtaca gctcagcagt
    18481 gcgaccaaca gtacgtctga aacgctggcg gcaacgccaa aggcagtaaa atcagcctat
    18541 gacaatgcag agaaacgtct gcagaaagac cagaacggcg ctgatatacc cgataaggga
    18601 cgcttcctga acaacattaa cgcggtcagt aaaacagact ttgctgataa gcgtggtatg
    18661 cgttatgtgc gggttaacgc tcctgcaggt gcaacatctg gaaaatatta ccctgttgtt
    18721 gttatgcgtt ctgctggctc agtaagcgaa ctggcatcaa gggtcattat caccacggca
    18781 acgcgaaccg caggcgatcc gatgaataac tgcgagttta acggatttgt tatgcctggt
    18841 ggctggactg acagggggcg ttatgcttat ggaatgttct ggcaatatca aaacaatgaa
    18901 cgagccatcc actcaataat gatgagtaat aagggcgatg atttgcgctc tgtgttctat
    18961 gttgatggcg ctgctttccc tgtttttgcg tttatcgaag atggcctgtc aatatccgca
    19021 cctggtgctg atctcgttgt taatgatacg acctataagt ttggggcaac aaatccggcg
    19081 actgaatgta tcgcggcgga cgttatcctt gattttaaga gtgggcgtgg tttttatgag
    19141 tctcattcgt taatcgttaa cgataacttg tcgtgcaaaa aactttttgc cacagacgaa
    19201 attgtagcgc gtggtggtaa tcagattcga atgataggtg gggagtatgg ggcattatgg
    19261 cgtaatgatg gcgctaaaac ttacctgctg cttaccaatc aaggtgatgt ttatggtggc
    19321 tggaatacat taagaccgtt tgctattgat aacgcaaccg gcgaactggt tattggaacc
    19381 aaactgtccg caagtctgaa cggtaatgca ttaacagcaa caaagctgca aacgccaaga
    19441 ctggtttctg gtgttgagtt tgatggttcc aaagatatta ctttaaccgc cgcgcatgtg
    19501 gctgcttttg ccagaagggc aacggataca tatgccgatg cggatggtgg cgttccctgg
    19561 aatgccgaat caggcgctta taatgtcacc cgctctggcg acagctatat tctggttaac
    19621 ttctataccg gagtcggaag ttgccggacc ttgcagatga aggcgcatta cagaaatggt
    19681 ggtctgttct accgttcttc aagagacggt tatggttttg aggatgactg ggcagaagtt
    19741 tatacctcga aaaatcttcc accagaaagc tacccagtcg gcgcaccaat cccgtggcca
    19801 tcagataccg ttccgtctgg ttatgccctg atgcaggggc agacttttga caaatctgct
    19861 tacccgaaac ttgcagccgc ttatccgtca ggcgtgatcc ctgatatgcg tggctggacg
    19921 attaagggca aacctgccag tggtcgggcc gtattgtctc aggaacagga cggcattaaa
    19981 tcgcataccc acagcgccag cgcatccagt acagatttgg ggacgaaaac cacatcgtcg
    20041 tttgattacg gcactaaatc cacgaataac actggtgcgc atacccatag tttaagtggc
    20101 agcacgaatg cagctggtaa tcacagccat agagatggcc gtcgatttaa ccccagtgtt
    20161 tttaaagata cttatcaata tggttataca agctcaggtc aaaatacctg gggtgtacaa
    20221 ggctcagtag gtatatctac ggggtggtta gcgaatacca gtacagatgg taatcatagc
    20281 cattcactgt ccggcacagc agcatctgca ggtgcacacg cgcatactgt cggtattggt
    20341 gctcatacgc actccgttgc gattggttca catggacaca ccatcaccgt taacgctgct
    20401 ggtaacgcgg aaaacaccgt caaaaacatc gcatttaact atattgtgag gcttgcataa
    20461 tggcattcag aatgagtgaa caaccacgga ccataaaaat ttataatctg ctggccggaa
    20521 ctaatgaatt tattggtgaa ggtgacgcat atattccgcc tcatacaggt ctgccagcaa
    20581 acagtaccga tattgcacca ccagatattc ctgctggctt tgtggctgtt ttcaacagtg
    20641 atgaggcatc gtggcatctc gttgaagatc atcggggtaa aacggtttat gacgtggctt
    20701 ccggcgacgc gttaatttct gaactcggtc cgttaccgga aaatgttacc tggttatcgc
    20761 cgggagggga atatcagaag tggaacggca cagcctgggt gaaggatacg gaagcagaaa
    20821 aactgttccg gatccgggag gcggaagaaa caaaaaacag cctgatgcag gtagccagtg
    20881 agcatattgc gccgcttcag gatgctgcag atctggaaat tgcaacggag gaagaaatct
    20941 cgttgctgga agcatggaaa aagtatcggg tattgctgaa ccgtgttgat acatcaactg
    21001 caccggatat tgaatggcca acaggaccca ttatcgaata gtgcattacg aactgcagga
    21061 tattatatgg gataatattc tgcagtttat tggggctgta ttttaaaaag gtgtttattt
    21121 tgagtgagtt atcaccttgt tctttccggt tcttttggct tgatataacg ctttatcggc
    21181 attgactaac atttcagatg gactgagatt attctctttt gaagtaaaac aaccaatact
    21241 gacggttatt ttctcaggtc catgcgataa taattttttc gagcattgct cctcaatgtt
    21301 ttttctgatt cgctctgcaa ttgatagcaa gagcttttga gtattacctt taatgataat
    21361 accgaactcc tcaccgccta agcgccctaa tatatcctct ttcctgatgg atttttttat
    21421 tattctggta accattacta tgacttgatc acccatatga tgcccccatt tgtcattgat
    21481 gcttttgaaa ttgtcaatat ctaac
//
LOCUS       DAMZII010000081        21449 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZII010000081 DAMZII010000000
VERSION     DAMZII010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.081"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..1109)
                     /locus_tag="POA77_002963"
                     /pseudo
     CDS             complement(1..1109)
                     /locus_tag="POA77_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1689..2702)
                     /locus_tag="POA77_002964"
     CDS             complement(1689..2702)
                     /locus_tag="POA77_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6366361.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
     gene            2809..3105
                     /locus_tag="POA77_002965"
     CDS             2809..3105
                     /locus_tag="POA77_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6366362.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(3239..3664)
                     /gene="arsC"
                     /locus_tag="POA77_002966"
     CDS             complement(3239..3664)
                     /gene="arsC"
                     /locus_tag="POA77_002966"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDI6366363.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3677..4966)
                     /gene="arsB"
                     /locus_tag="POA77_002967"
     CDS             complement(3677..4966)
                     /gene="arsB"
                     /locus_tag="POA77_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDI6366364.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(5020..5373)
                     /gene="arsR"
                     /locus_tag="POA77_002968"
     CDS             complement(5020..5373)
                     /gene="arsR"
                     /locus_tag="POA77_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDI6366365.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            6113..6196
                     /locus_tag="POA77_002969"
     CDS             6113..6196
                     /locus_tag="POA77_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDI6366366.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(6250..7602)
                     /gene="gorA"
                     /locus_tag="POA77_002970"
     CDS             complement(6250..7602)
                     /gene="gorA"
                     /locus_tag="POA77_002970"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDI6366367.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7674..8516)
                     /gene="rlmJ"
                     /locus_tag="POA77_002971"
     CDS             complement(7674..8516)
                     /gene="rlmJ"
                     /locus_tag="POA77_002971"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDI6366368.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8719..10761
                     /gene="prlC"
                     /locus_tag="POA77_002972"
     CDS             8719..10761
                     /gene="prlC"
                     /locus_tag="POA77_002972"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDI6366369.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10767..11519
                     /gene="rsmJ"
                     /locus_tag="POA77_002973"
     CDS             10767..11519
                     /gene="rsmJ"
                     /locus_tag="POA77_002973"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDI6366370.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11568..13037)
                     /gene="dtpB"
                     /locus_tag="POA77_002974"
     CDS             complement(11568..13037)
                     /gene="dtpB"
                     /locus_tag="POA77_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDI6366371.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(13355..13789)
                     /gene="uspA"
                     /locus_tag="POA77_002975"
     CDS             complement(13355..13789)
                     /gene="uspA"
                     /locus_tag="POA77_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDI6366372.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            14180..14515
                     /gene="uspB"
                     /locus_tag="POA77_002976"
     CDS             14180..14515
                     /gene="uspB"
                     /locus_tag="POA77_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDI6366373.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14586..16085)
                     /gene="pitA"
                     /locus_tag="POA77_002977"
     CDS             complement(14586..16085)
                     /gene="pitA"
                     /locus_tag="POA77_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDI6366374.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16317..17510
                     /locus_tag="POA77_002978"
     CDS             16317..17510
                     /locus_tag="POA77_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6366375.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAV"
     gene            17622..18443
                     /locus_tag="POA77_002979"
     CDS             17622..18443
                     /locus_tag="POA77_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HDI6366376.1"
                     /translation="MGRRRKLETLDDFQRALKNKYGLGESDSYKPWLRVQDVRSIGNS
                     AKIQGIKAKREHHMLSEHESCFFYIAEFCDSVIDIREQFPLFPLDLSVKIAKTFGVKH
                     PVVPGTKSPNVMTTDFVLTRSDGVNTWYEAVSVKPSEKLTDVRVAEKLDIERIWWQLL
                     GVPFHLFVMTKENQIQSKNIQWITAPVRQGRRFSTDLVERSSALISPGTILIEDICDE
                     FVREFDLEHDDALVLLKTLIATKVVNVDLNKPIADIGILNIIKVQDFKVVRHHAS"
     gene            18433..20583
                     /locus_tag="POA77_002980"
     CDS             18433..20583
                     /locus_tag="POA77_002980"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDI6366377.1"
                     /translation="MQVERNSIWLITRVDGIKDGKYRVLAQYANYDLLILYRLHDEKC
                     LEMPVPVKYPLFLESAKCGNSKISSYLTPFYQLISEEDISPAHRVKRNNRFELINDLI
                     SNPEFLLDVALNQRSKIVASHAKERGVYVQKIYRSLNQYWQYGQEINALLPAYKHCGG
                     SGKYRLSGIAKRGAPIQLSTPSMVAPLGVNTTEEDKTKFLLGMKKYALRGKKVSLNRV
                     YEQMLKEFYADELQLAETEHREPALPSLRSFRYWIKRLVPEAELIRKQTTAGDFERNR
                     RGLRGSATDHTEVPGSCFELDATVLDVHIVSEFNRNHVLGRPTVYCVVDKESRMIVGL
                     HVSMEYASWRAGRQALVNSFTSKKAYCARFGIDIDESEWPCHHIPQRLLCDRGEFICN
                     KPEELAVPLIGHLSIAPPYRADLKGIVEHRFHILNEKLIHELKGTTRGRHYIRGDRDP
                     RLDATLTLTEVTRLLIDQVLEHNSSIFDGLAGQTLLLVEASLPPTPLNYWNIHLKKHR
                     HALNKADEADIRARLLPVEQVSMTSKGVRLNDDMYYECDREEFEDWKVIARTNGRWKL
                     EARFDQDNASFIYVRFRPNEGFTRCQLMLHSANFEARHRADVLYFEDWKKVVKKRSTP
                     TGKSIERHNRRKAITVFAQEELKREPALNSKSEKISNIKERRRDAIMDARLTSDDDFA
                     ELHERESSVDCIAIEKASNVITLLKRARSPNNEN"
     gene            20573..>21449
                     /locus_tag="POA77_002981"
     CDS             20573..>21449
                     /locus_tag="POA77_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6366378.1"
                     /translation="MKIEKAVYRPAILPEHRGNPLIEALPTKYTDEELVDRLSFYPSH
                     SFDETQLDPFERVEYLTRLKDLRQPLPIYLDCFRAVEMAIKEGYSVKNPLSPTTMNYL
                     HYPIDRRPNIEPRTGYFKPKGCGITVIGESGVGKTCMLEQILGCFPDTIEHTQYKKHS
                     LPLRQVVWIKVDCPDDSSVRALCHKILSELDSKLGFEPTKRAGTIPLLLDQIEARIKS
                     SFLGILVIDEMQNLNLAKTGGADRLLGFIHNLVNNLGIPILFCANPPFNQLLSRTLKA
                     ARRAESNGYFDVVLME"
BASE COUNT         5656 a         5067 c         5375 g         5351 t
ORIGIN      
        1 aggcgtgtgt gacatttttt tggggttaag cgtctcgtat tgaaatactt cgaactgata
       61 ttcagacatt tctgcccatg tttgaatttt attgtatcga gagagtgtcg caaaggcttt
      121 aacttgctta taacattgtt tttggaattc ttgctgatat gttaatatgt cattgtatga
      181 aagattggtt atcctagcct ctaaaaattt aattattgtc gaagcaattt ccgcttttgc
      241 tcgtaaaaag ttgtcattaa tggttaataa tgttgaaaat aaaccttcga tttgtttgtc
      301 aagatattgt ccatctttat ataatttggg ggtagggtgt tctttatgta aaaaatagtg
      361 tcacctaaat ttgcgtcagt gaaatttata ttaagtagat cttcgaaata aaaccttgac
      421 aaggttagaa cgaacccgga caggttctgt cctgtcatat tgaaatcaat aaagttggtt
      481 ccggcaaaga ctttcatcct gtttttattt tcactgtgct caatatataa cttcgtcatt
      541 ttttgataca ggctttgaat atcttttata ttagttggta agtcagctcg ttgttcagca
      601 ataaaattag tatctgaaaa catggtttgg acactgaatg ttatgggtat tccctcttct
      661 acgctaattt tagttatgat tgccttgtcg ggtttaggat tagcgacaac aatgattcga
      721 cattcagcac ttttgttttt ttcatatcta atgttattaa tctgattaag tattgattgt
      781 gtctgttgcg taagttgtgt cgaacttatc gtttcaatgt tagtaataag acttacaagt
      841 tgagccacaa cttttgagta gtctgcattt gtatatgtag tgttcgtgtg gcacgcatta
      901 atttgttgaa gttggtgttg cgctaaatca tattgatcag agatatatcc ttctccagag
      961 aatgtggaat taatgggttg ttgttcttcc caattatcgc aaggccactt cggtgtgtct
     1021 ggcgttttag cattatcggt atttgttgtg cagttataaa tatcgccgtg atatgtattt
     1081 attatacctg gggtaaagtc aattgacata aaattcctta ttcatcattt gttttatgct
     1141 aagatgttta aattggataa ttagaacagg aatggatttt cttctacaca caataaatat
     1201 aatgcgttat atcttcctga tataattacc tttaaattcc ctttcatttc cacccggtaa
     1261 tatttgtgta taaaaaaagg cagtgatagt atcgtaaagg aaaacgttaa caactatcaa
     1321 aaagcgctca ttttaaaaag ttattttgtt ctccgatagt gtgtgaagta atatttttgt
     1381 gatgttttag atgtgtaata atgaaccagg ttaaagtgta tttaaaaatg tgttgtatta
     1441 gcgactgaaa tctgcctttt aaaaaggagg agggtactga gacaaagagt cattgcttct
     1501 ccgataaaat gtcagagaag catgattacg tggttattgt ctgttgatga cttgttgacc
     1561 gagccgtcat cattctcaat gcattagtta aatcatttgt gatttttaag gattatcctg
     1621 tctatgattt tctacttaga aggatatggc actaactatg catcttatag tttactgctg
     1681 gcatctattt aaaatagcaa ttgagtcaaa tagcccatca agatagccat acctaatata
     1741 accgtcagga atgcagctat catcggcatt ctgaacattg atttaagtaa tatcacttcc
     1801 gtcaggcttg cgccggcact gccgatgatt aatgccatta atgctcccag acccattcct
     1861 tttgtcatca gaacagatgc cagagggata accgcctcag cccggatata cagcggaata
     1921 ccgacaacgg cgcttaatgg gatggcgaag ggattatctg ctcctgcatg agcggcaatc
     1981 cacgctgagg gaataaagcc ataaataaaa gagcctatta atacccctaa caaaagatat
     2041 ggcagtacat ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata
     2101 cttgccactg ctttggtatt gcaacagtta acgacgggtt tctcaatggc tttagccgtt
     2161 ggactacagc aactcacttc tattggcgta tatgtcgtcc ccggcaaagt ttttgccgac
     2221 gctttggctg gagcacaaca atttgctgat gagcttttac tggcaatgat atgacgttca
     2281 aaccccagag aatccaggat aatactggca agaacggaga cgccggtggc gataatcgcg
     2341 tacaacaagg tgactttcca accaaaggtc acccacatta gtccgacgat aatgggatta
     2401 agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa acccggcttt cgctgacaac
     2461 aatccacgta gcatgggtat tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga
     2521 gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat tttatggtct
     2581 ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag caacgaaagc
     2641 tcgacggcga gaaacacaaa catctctgcg gcatcttgca gcatcgcaag ccaactactc
     2701 atacgtaacc tcattggata aatctatatt tccagaataa tcgaaatgaa gttcagatca
     2761 agatatttct ggtattatcg aaatatcaac cagacagagg ggtgtcatat gcagttggaa
     2821 gaagtcgcta aagcgctgaa agagctaggt cacccgaccc gtttgttcat atttaaacat
     2881 cttgtcaaag ccggggaaca agggttgccg gtcggtgagt tgcaaaagca gcttgggata
     2941 cctggctcca cactcagcca ccatatatca gcactggtat cggtaggact ggtcaaacaa
     3001 aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg caatgctgga agcgataatt
     3061 gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac
     3121 atatttcatg ccatatattg aatttgcacg gttttgtagg tcggataaga cgcgttagcg
     3181 tcgcatccga cataaacaaa gcgcacttta ccaactatgg gcagtggata tcgccgcttt
     3241 atttcaaccg cttacccgct tcatcaacca ctttctcgcc atcttccttg gagaatgcgc
     3301 ctttttgcgc atctggcaga atttccagca ccacttctga agggcggcac aggcgagttc
     3361 ccagcggcgt caccacaatc gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga
     3421 ttaaccgatc gtcagtaaat ttatcttctg caaggcccag ttcctcatac ggctcgacgt
     3481 ttttacgcaa cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg accagctcat
     3541 cgcgcgttgg cggtgtttcc agataataga taatggtcgg ttctgtgccg ctgttgcgga
     3601 tcatctccag cgtattacgc gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc
     3661 tcatatcagt atctcattac aaagtgaaag agagacgtag cgccagcgca gccagcgtca
     3721 caaacagcac aggcagggtc atgataatcc ctgtacggaa gtaatatccc cagctgatag
     3781 tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt agccaggcta ccaattgggg
     3841 taattttcgg tcccaaatcg cagccaatca cattggcata aaccatcgct tctttgataa
     3901 cgccagatgc cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc ggcatattgt
     3961 tcataataga agagaggaag gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc
     4021 cgttatccgc cagcacgttg agtacgccag aaagatattc cgttaatccg gcattgcgca
     4081 ggccataaac caccagatac atgccgagcg agaagatgac aatctgccag ggggcaccgc
     4141 gcaggacttt acccgtatta atcgcatgac cgcgtttagc gacgacaaat aatatcagcg
     4201 cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca
     4261 ccagcagaag cagtaaaaca acccagccag ctttgaacgt agcaggatct ttgatcgctt
     4321 ctgcgggaga tttcagcagc gccatatcgt agttctgcgg aatatctttg cgaaaataga
     4381 gatgtaacat caccagcgtg gcaacaatcg cggcgatatc caccggcacc atcaccgagg
     4441 cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac gatattcacc aggttggaga
     4501 caataagcgg caggctggcg gtatcggcaa tgaatccggc cgccatcacg aacgccagcg
     4561 tagtgccttt actgaaccct aaagccagca gcatggcgat gacaatcggt gtcaaaataa
     4621 gcgccgcgcc atcattggca aacagggcgg caacggcagc accgagcagg acaatccagg
     4681 taaacagcaa gcgaccacga ccattacccc agcgtgagac gtgcagcgcc gcccattcaa
     4741 aaaagccgga ctcatccagc agcaggctga tgataatgac ggcgataaac gcagccgtcg
     4801 cgttccagac gatattccac accaccggaa tatcacccgg atggaccacg cccgtaacta
     4861 acgccagtac tgcgccgagc gttgcactcc agccgatgcc taaacctttc ggctgccaga
     4921 taaccaatac gatggtcagg acaaagatag cgcctgccag taacataatg cctcccggat
     4981 aaaacacatc tgaaaattca tatgtgttta gctaaatttt taactgcaaa tgttcttact
     5041 gtccccggaa cagttttgtc gagccaggtt gcggacaatc gcctgaacct tttcctgttc
     5101 acatcgccag gcctcatcaa taattttcgc cgcccatgct ggaatatgcg gtgataagcg
     5161 gtaatgaacc cacttacctt gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc
     5221 caggtggcgg gagatcttgg gctgcgactg gtcgagagca gtgcagagat cgcagacgca
     5281 taactctccc agttcgctga gcagtaaaac gatgcccaga cgggtttcat cagcaagaat
     5341 tttgaacaat tggatgggta acagaaatga catattgcgc tcctgattgt tgcaggtagt
     5401 gtctctcttc gaagcggata agtcaaaaac atatatgact taacgaatgt gtaagtgcag
     5461 aggaaggtaa taggtgtgaa ttttgagttg gctattcatt tgaaaagagg tctgaatcag
     5521 gacgtaaaaa aaccgacttt gcgtcggttt tttactttcc agccctgagt tggtggctct
     5581 ggctggagtg agagagttct tatctaacag ctcaatacta ttagccaatg gctaacattg
     5641 caagaattag cgatttcttc agctggcgga gggggcactt cggtatatat cgcaatgacg
     5701 atttatatac tttcactggg tcatcgtcat attaagcctt gtccgaatcc ggatgtaaaa
     5761 aaaccgactt tgcgtcggtt ttttactttc cagccctgag ttggtggcac tggctggagt
     5821 gagaatgcca ttatctaacg catcaatgct aataccatac tgaaactatt gcaaggacat
     5881 gctggtttta taacctgaat gtactgtatg attatccagt tagctctgag gcattttcac
     5941 tctggtaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa
     6001 ctgatgctct ggccggagtg agaatgccat tatctaacaa tagggcatgc gccgtgacag
     6061 gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga
     6121 taaagcaatc atcgttcttg gggcgttaat tgcgctgctg gaactgatcc gctttctgct
     6181 tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact actcttagcc
     6241 ctttaacatt taacgcattg tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt
     6301 attgtcgaag tcttttttgg ttgcacccat cttcagcgcc accgcgaagc cctgcaacat
     6361 ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttccg atccaacgca
     6421 caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa
     6481 agaggattta tacactttca cctgatcgtc gccatactgc tcgcgcgcct gcggttccgt
     6541 taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc
     6601 cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac ccgctgcaac
     6661 tgccaccggt gtcagctcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaat
     6721 attggtgttt tgatatttat cgacgacgat atagcctttt tcgttagttt taacgccagc
     6781 ggcttccagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc
     6841 caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg catttttcac
     6901 taccgctttc gggatggcgt tggtgtgcag ctgcgggcct tcggcgttca tcacttcgac
     6961 cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag
     7021 atgcgttttc gcgccgaggc cgttaatcac gcccgccagc tcaacggcga tgtaacccgc
     7081 gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat cagaatcaat
     7141 accgtattcc acgcccggaa tatccgggtg gctcggacga ccgcctgtgg cgatcagaat
     7201 atgatcggcc gtgatggttt cgccgtttac ctccagcgtt ttggcatcaa cgaagcgggc
     7261 aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg aagtatgaat
     7321 acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaatt tattgatagt
     7381 ggtatcaaaa ccataatccg ggccgtacat atggatcgct tcacggattt gcgccgcgtg
     7441 ccacatcact tttttcggca cacagccaac atttacgcag gtgccgccca gctctttggc
     7501 ttcaatcagc gcacatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc
     7561 gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacaaagt gtccttatcg
     7621 ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactcc
     7681 ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg gaaccagttt gctgtgcagc
     7741 cacggcagca cgttattcat ctgctgttcc agtttccacg gcgggttaat cacaatcatg
     7801 ccggaagcgg tcatgccacg gcgatcgctg tctggcagta ccgccagttc aatttgcaga
     7861 attttgcgaa taccggtcgc ttccagatcg tggatcatgc gcttaatttg ctgacgcagc
     7921 accaccggat accacagtgc gtaagtacca gtggcgaaac gtttgtaacc ttctgctatc
     7981 ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat aaggattaaa
     8041 ccacggcggg aaaccggcgg cagtttggcc ttaagctgct ggaaaccatc ggctttttcg
     8101 acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca acgggtaatc gctcgggtgc
     8161 agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat caatggcgaa
     8221 ccggggtagt aacgcaactg accgctacgg ttgaagtgtt ttaccacatt gatgtacgcc
     8281 tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgatgccttc gagatattcg
     8341 ccggtacgct cggcatgttc gctgcctaac tgataacgcc cggcccctgc gtgggtgtcg
     8401 agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct ctgaacggta
     8461 tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt
     8521 aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag cgtatcagga
     8581 cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta
     8641 cacttaaccc catgctacac acattatgta aagcgcctgt tgagcgcttc cttaacctct
     8701 ttaaccagga ctgcgcgaat gacgaatccg ttactgactc cctttgaatt gcctccgttt
     8761 tctaaaattc tcccggaaca tgtcgttcca gccgtgacta aggcgctgaa cgactgccgc
     8821 gaaaatgtgg agcgcgtagt agcgcaagga gcaccgtaca cctgggaaaa tctctgccag
     8881 ccgttggcgg aagtggacga tgtgctgggg cgtatcttct ccccggtcag ccacctgaac
     8941 tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtcg
     9001 gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtatcg cgacctgcgc
     9061 gatggcgatc attacgccac gctgaacacg gctcagaaaa aagcggttga taacgcactg
     9121 cgcgacttcg aactctctgg catcggtctg ccaatagaga aacaacaacg ctacggtgag
     9181 attgccactc gtctttccga gctgggcaac cagtacagca acaacgtcct cgatgcgacg
     9241 atgggctgga ccaaactcgt taccgacgaa gcggagctgg cggggatgcc tgaaagcgcg
     9301 ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct gctgacgctg
     9361 gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggcctt gcgtgaagag
     9421 atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg taaatgggat
     9481 aacagcaagg tgatggaaga gatcctcgct ctgcgtcacg aactggcgca actgctgggc
     9541 tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg
     9601 ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggca
     9661 caactgcgcg cctttgccaa agccgaattt ggcgtcgatg agttgcagcc gtgggatatc
     9721 gcgtactaca gcgaaaaaca aaaacagcac ctctacagca tcagtgacga acagctgcgt
     9781 ccgtacttcc cggaaaacaa agcggttaac ggtttgtttg aagtggtgaa acgtatttac
     9841 ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc acccggatgt acgtttcttc
     9901 gaactgtatg acgagaacaa cgaactgcgc ggtagcttct acctcgacct gtatgcccgt
     9961 gaaaacaagc gcggcggggc gtggatggat gactgcgtag gccagatgcg caaagctgac
    10021 ggttctctgc aaaaaccggt cgcgtatctg acctgtaact ttaaccgccc ggtaaatggt
    10081 aaaccggcgc tgtttaccca tgacgaagtg atcaccctgt tccacgagtt cggtcatggt
    10141 ctgcatcata tgctgacccg catcgaaacc gccggtgtat ccgggatcag tggggtgccg
    10201 tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga gcctgaggcg
    10261 ctggcgttta tctctggtca ctacgaaacc ggcgaaccgc tgccgaaaga gttgctggat
    10321 aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgcca gctggagttc
    10381 ggcctgttcg atttccgcct ccatgccgag ttccgcccgg atcagggagc gaaaatcctc
    10441 gaaactctgg cagaaatcaa gaaactggtt gccgtagtac cgtctccatc ctggggccgt
    10501 ttcccgcacg ctttcagcca tattttcgcc ggtggttatg ccgcaggtta ctacagctac
    10561 ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga gggcattttc
    10621 aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaggag
    10681 ccgatggatc tgttcaaacg cttccgtggt cgtgagccgc agctggatgc gatgctggag
    10741 cattacggga tcaaaggtta gtttttgtgc agatttgctt aattgatgaa acaggcaccg
    10801 gagacggtgc cttatctgtt ctggcggccc gctgggggct ggagcacgat gaagacaacc
    10861 tgatggcgct ggtgttaacg ccggaacatc tggaattgcg caagcgtgat gagccaaaac
    10921 ttggcggcat ctttgttgat tttgttggcg gagcgatggc gcaccgacgc aaattcggcg
    10981 gtggtcgcgg tgaggcggtg gcgaaagcgg tgggcattaa aggcgattat ttgccggatg
    11041 tggtggacgc caccgcaggg ctggggcgcg atgcctttgt gctggcttca gtcggttgcc
    11101 gcgtgcggat gctggagcgt aatccagtcg ttgccgcctt gctcgacgac ggcctggcgc
    11161 gtggttatgc ggatgcggaa atcggcggct ggttgcagga gcggttgcag ttaattcacg
    11221 cctccagcct gacggcgctg actgatatta ccccgcgccc gcaggtggtt tatctcgacc
    11281 cgatgttccc gcataagcag aaaagcgcgc tggtgaaaaa agagatgcgt gtctttcagt
    11341 cgctggtggg gccggatctt gatgccgatg gattgctgga gcctgcgcgg ctgctggcga
    11401 ccaaacgcgt ggtggtaaag cgcccggact acgcgccacc gctggcgaat gtcgccacgc
    11461 caaacgcggt agtcactaaa gggcataggt ttgatattta tgccggaacg ccggtgtaaa
    11521 agtaaagcag ggtagcgata acgctaccct gctttctgca agaataatta atggctttcc
    11581 ggcgtcgcaa tcatgcgttt cagccacggc accatcaaca gcatcactac tgcaacgccc
    11641 agcgtgacca gaccaatctt accaaacacg ttggtataga cgggcaacgt ctcaagcgga
    11701 tcggtaatgt tgtccggcac cgcggtaaat gttgccacat agccgcccag caagaacgcg
    11761 gcagcctgcg tcaggaacca catcccgaga ataaagccca tcaaatgctg cggcaccagg
    11821 gcagcaatca tcgccagacc cagggcgcta ataaacaatt cacccaggct ctggaacaag
    11881 taaaccagca cgataaacca cggcgatgtc agtccttgtg catcagcaaa ccacattccc
    11941 gcagctgccg ccgtcaaaaa gcccagtgag cacataaaca tgccgagagt aaatttcatc
    12001 ggcatcgaga ggtctttgcc tttgttaccc agatgcgtgt aaatgcctgc cagtattggg
    12061 ctggcgagta ccacccagaa cgggttaagc gcctggaagc tgaccgggtt gatggaaaag
    12121 ccgagaattt catgatgcac gttgttgatg gcaaagaagt tcagcgacgt tggcatctgg
    12181 gcgtagagaa tgtaaaacac caccgcttcg agcatcagga caaaggcgac aaacatttta
    12241 ttgcgcccgg ttttatccag cttgaatgcc tgacgaaaga agatgatggt gacgacgatg
    12301 gagagaacaa tcagcaccag attggcgact tctacgttgt gcatcagcca tgcacagacg
    12361 aagatcatca ccacgctgcc aagtaacacg tacaacagtt tgctgaagct cattggccgg
    12421 aagtcgggtt cagaaccaat gtctttcacc attccacgac aggcgatgta aaccagtaat
    12481 gcgataatta accccgcccc gcacaggttg taggtgactg aataaccgaa tctatcagcg
    12541 atcacagggg ccagcgataa cgctatcaac gagccgatat tgatcgacat atagaacagg
    12601 gtgaatgcgc catcaagccg cggatctttc ggcggatagc acttcgaaag caagctggct
    12661 gggttagctt taaacaggcc gttaccgaca gcgatagtcc ccagggcgat gaaaatcagg
    12721 tcaggcttaa gtagcgacat gccggtcatg aagtagccaa tcgccagcac aagtgctccg
    12781 agaacaatgg tgcgtttggt ccccagcagg tggtcgccga catagccgcc aatggaaatg
    12841 aggccataga ccagcgcagc aaaagcacca aaagtgacaa aagcctgctc ttgcgagaat
    12901 ccaagctgtt taacgaagaa aaccgccagt acgccctgca cgccgtagta gccgaatcgc
    12961 tcccataact cgacaaaaaa gatcatgaaa aatgggcgag gttgctgcag catccccatg
    13021 ggtgttgttg tattcatatt tattaacctt ccaataccat cctgaaaagt atgaacgcgt
    13081 aagggataac gcgcgaaaaa gtttacatct gtcagagatt cttaatattg atgcggtgaa
    13141 tagattaata cactattaca acagaaaata accagctgat cacactaatg atggaaaata
    13201 tgatggtgtt aaatgttgta ttggcaggag attttgattg cgtacgcaga tcgtaggcca
    13261 gataaggggt ttacgctgca tcaggtaaca aggcgcgcca aaaagcaaaa cgcccgcata
    13321 tgcgggcgtt caggaacacg tcgtgggggg atgattattc ttcttcgtcg cgcagcggaa
    13381 caatcagcat atcaacgtga acggtgttga tcagctgacg tgcggaagac atcagtttgc
    13441 tccagaagtc ctggtggtga ccacaaacca ccagatccat atcgtatttc ttgattgcat
    13501 cgaccagaac ctggcccagg tcgccgctgc cgctcagggt ttcagtgatt gggtagcctg
    13561 cattagtgga aagctcggtc agcgcatgat gtgtctcttc agagatgcgt ttctgcatat
    13621 cacccagatt cacatcaata agcccggtgt ataggtcaga gtagtttaca tctacgtgga
    13681 tcagagaaac tttcgcattg taggggcgag ccatagagac tgctttctct accagaactt
    13741 tgctttccgg ggagagatcg actgcgatga gaatgtgttt ataagccata gtgttactcc
    13801 ttccataaag ttgtcgatga ctggccagct agcgtttctt gtgccgttac gtcacccgcg
    13861 tcctgcgaac tacgtgcgcg gggctaaccc agccgtaaag attcagtgtt aagaccatcc
    13921 ttaccttata gcgacccgga tgatccgtca atccgccttg cttacccgtt agataaacaa
    13981 attttcgaca tgaatatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc
    14041 ctacactatc tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc
    14101 tggtgttaac tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc
    14161 aggtcgccgg ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt
    14221 ttgcattgtt aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg
    14281 taactgcgat ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg
    14341 ccaacccaac aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca
    14401 tcacgatgat gaatttattc gccgctgtga gcgggtgcgt cggcagttta ttctgaccag
    14461 cgcattgtgt ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact
    14521 tcagcaataa taacgcataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgtg
    14581 tacgattaca ggaactgcaa ggagagccag tacagcccgc cggaaagcag taccgcagcc
    14641 ggaagggtaa acacccaggc catcagaatg ctggtcacgg ttttacgctg taagccgcca
    14701 ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc
    14761 atcccggtat aactcgccag gccgatagac actgccgccg tcatctgggc agacatcccc
    14821 tgagcgtagg tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacggcgc
    14881 cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggt
    14941 gcatactcga tggtgctaag catgtcagac ttcagtttct tcaacaggcg ctgatcgtca
    15001 gcactcacgc caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg
    15061 cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc
    15121 aacatacctt tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc
    15181 gcaggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt
    15241 ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta
    15301 cgggtgattt cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca
    15361 atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac
    15421 gccacgccga tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg
    15481 ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg
    15541 ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg
    15601 gaaacgatca gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact
    15661 gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta
    15721 tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc
    15781 agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc
    15841 gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa
    15901 aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata
    15961 acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca
    16021 aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt
    16081 agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc
    16141 ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca
    16201 aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt
    16261 gcgaccactg aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg
    16321 aaaggtttga tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg
    16381 caggtcaggc aggacgccgg gttctgctga tcgataatgg taaaaaacca gggcgcaaaa
    16441 tccttatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt
    16501 atctgagcca gaatccgcat ttttgtaagt ctgcactcgc gcgttttacc cagtgggatt
    16561 tcattgatct ggtcaataaa cacggcatcg cctggcacga gaaaacgtta ggacaactct
    16621 tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca
    16681 atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca
    16741 cgcttgaact gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc
    16801 tgtcaatgcc ggggctgggc gcgtcgccgt ttggttataa gattgccgaa caatttggcc
    16861 tcaacgtgct gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg
    16921 aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca
    16981 tcgttttccg tgagaactta ctcttcaccc atcgcggctt gtctggaccg gcggtgttgc
    17041 agatttccag ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg
    17101 atctcgaaac cttcctgaat gagcagcgta acgcacatcc gaatcagagc ctgaaaaaca
    17161 cactggcggt tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc
    17221 cggatgtttc gctaaaacag ctcaacgtgc gtgaccaaca ggcactgatt agcacattga
    17281 ccgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg
    17341 gcggcgtgga caccaacgaa ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc
    17401 tgtacttcat cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt
    17461 gggcgtggtc gagtgcgtgg gcttgtgcgc aggatttgat tgctgtgtag tgacaataaa
    17521 gtttgtacat tgcttataaa gtttgtacac aaagctcttt tgtaacaata aagtttgtac
    17581 actaaactat gcttcataca tttgttatgg agtgaagttt gatggggcga cgtagaaaat
    17641 tagaaacact ggatgacttt caaagagcct taaagaataa atatggtctt ggtgaaagtg
    17701 atagctacaa gccatggctt cgggtacaag atgtccgctc tattggtaat agtgcaaaga
    17761 ttcagggtat taaggcaaaa cgagagcatc acatgctttc tgaacacgaa agttgctttt
    17821 tctacattgc agagttctgc gattccgtta ttgatatcag ggagcagttc cctctcttcc
    17881 ctctagacct atcggtaaaa attgccaaaa cctttggggt gaagcatccc gttgtacctg
    17941 gaaccaagtc gcctaatgtt atgaccacgg attttgttct gactcgctct gacggggtga
    18001 acacctggta tgaagctgtg agtgtcaaac cttcagagaa actgacagat gtaagagttg
    18061 ccgagaaact ggatattgaa cgtatctggt ggcagttgct gggagttcca tttcatcttt
    18121 tcgtgatgac caaagaaaat caaatccagt caaaaaacat tcaatggatt acggctcctg
    18181 tccgacaagg ccgaagattt tcaactgact tagtcgaaag atcttcagca ctgatttctc
    18241 caggaaccat actgatagag gatatatgtg atgagtttgt tcgagagttt gatcttgagc
    18301 atgacgatgc gttggttcta ttaaaaacct tgattgcaac caaagtggtt aatgttgatc
    18361 tcaataagcc aatagcagat ataggtattt tgaatattat caaagttcaa gactttaaag
    18421 tggtgcggca tcatgcaagt tgaacgtaac agtatttggc tgattactag agtggatggt
    18481 attaaggatg gcaagtacag agttcttgct cagtatgcaa attatgacct cttgatcctt
    18541 tatcgactac atgatgaaaa atgtttggaa atgcctgttc ccgtaaaata tccactattt
    18601 ttagagtctg caaagtgcgg gaacagcaaa atatccagtt atttgacacc tttttatcaa
    18661 ctgatctctg aagaggatat ttcccctgct cacagggtaa agcggaataa ccgtttcgag
    18721 ttgataaatg atttgatcag caatccagag tttttgcttg atgtagcctt aaatcagcgt
    18781 agcaaaatcg tagctagcca tgccaaggaa cgaggtgttt atgtccaaaa aatctatcgt
    18841 tcactgaatc aatattggca atatggacag gaaattaatg cattacttcc agcgtacaaa
    18901 cattgtggtg gctcaggtaa ataccggcta tccggtatag cgaaacgagg ggcaccgata
    18961 caactgtcga cacccagtat ggtggctcct ctaggagtaa atactacgga agaagataaa
    19021 accaagtttc ttctaggaat gaagaaatat gcgttaaggg gcaagaaggt ttcgcttaat
    19081 cgtgtatatg agcagatgct gaaagagttt tatgcagatg agcttcaatt agccgaaact
    19141 gaacatcgag agcctgcgct ccctagtttg aggtcatttc gatactggat taaaaggctg
    19201 gttcctgagg ctgagttgat ccgtaaacaa actacggcag gtgattttga acgaaatcgg
    19261 agaggtttac gaggatctgc cacggatcat acagaggtgc ctggcagctg ctttgagctg
    19321 gatgcgacag ttcttgatgt ccatatagtt tcagagttta accgaaatca tgtgcttggt
    19381 cgtccaacag tttactgcgt agtcgataaa gaaagtcgga tgattgttgg tcttcatgtc
    19441 tcaatggagt acgcttcttg gcgagccgga agacaggctt tagtcaatag ctttacttcg
    19501 aagaaggctt attgtgcgcg ttttggtata gatattgatg agtcagaatg gccttgccat
    19561 cacatccccc agcgtctcct gtgtgaccga ggggaattta tctgtaacaa gccggaagag
    19621 cttgctgtcc cattaatagg gcatttgagt attgctcctc cttatcgtgc agacctgaaa
    19681 ggtattgtag agcacagatt tcacatcttg aacgaaaaac tcatccatga actgaagggg
    19741 actactagag gacgccatta tattcgtgga gacagagatc ctagattaga tgctacttta
    19801 actcttactg aagtgactcg gcttcttatt gatcaagttt tagagcataa cagttctatt
    19861 tttgatggcc tagctggtca aacccttttg ctagtagaag ccagtcttcc tcctacccct
    19921 ttgaattact ggaatataca tctaaaaaaa caccgtcatg cactgaataa agcagatgaa
    19981 gcagacatca gggctaggtt gcttcctgtc gagcaagttt caatgactag caagggagtt
    20041 cgtcttaatg atgatatgta ttacgaatgt gatcgggaag agtttgaaga ctggaaagtt
    20101 atagctcgta cgaatggacg ttggaagctt gaagcgcggt tcgaccaaga taatgcctca
    20161 tttatctacg tacgctttcg cccaaacgaa ggatttactc ggtgtcaact gatgttgcat
    20221 tctgccaatt ttgaagctcg tcatagagct gatgtactgt atttcgaaga ttggaagaaa
    20281 gttgttaaga aacgttctac accaactgga aaatctattg aaagacataa tagaagaaaa
    20341 gcaattactg tatttgctca ggaagagcta aaaagagaac cagctttaaa ttcaaaatca
    20401 gagaaaatat ctaatatcaa ggagagaaga cgagatgcca taatggatgc taggcttacc
    20461 agtgacgatg actttgcgga gcttcatgaa cgtgagtctt cagttgattg tattgctatt
    20521 gagaaagcca gcaacgttat cactttgctg aagcgtgcgc ggagtccaaa taatgaaaat
    20581 tgaaaaagca gtttatcgcc ccgctattct ccctgagcat cgaggaaatc ccttaatcga
    20641 ggctttacca acaaagtata cagatgagga gcttgtagac agactcagtt tttacccgtc
    20701 gcactctttt gatgagactc aacttgatcc ctttgaacga gttgagtatc tgaccagact
    20761 gaaggatcta cggcaaccat tacccatcta tctggactgt tttagagcgg tggaaatggc
    20821 aataaaagag ggttactcag tcaagaatcc gctttcaccg acgacaatga attatttaca
    20881 ttatccaata gatcgtcgtc caaatattga accaagaact gggtatttca aaccaaaagg
    20941 gtgtggaatc actgtaatag gtgaaagcgg tgttggaaaa acctgcatgc tcgaacagat
    21001 attaggatgt ttccccgaca caattgagca cacgcaatat aaaaaacact cgctaccatt
    21061 acgccaagta gtatggatca aagtggattg ccctgatgac tccagcgtta gagctctgtg
    21121 ccacaagatt ctgtccgaat tggatagcaa gcttggcttt gagccgacta agcgagccgg
    21181 aactatccct cttctgttag accaaatcga agctcgtatc aaatccagct ttttagggat
    21241 cttggtcatc gatgagatgc agaatctgaa tctggcaaaa accggtggtg ctgaccgcct
    21301 gttgggtttc atccacaatc tggttaacaa tctcggtatt cctatcctgt tctgtgccaa
    21361 cccaccgttc aatcagttgc tcagtcgaac tcttaaagcg gcacgacggg cagaaagcaa
    21421 tggttatttt gatgtcgtgc tgatggaaa
//
LOCUS       DAMZII010000082        21201 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMZII010000082 DAMZII010000000
VERSION     DAMZII010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.082"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            155..1240
                     /gene="mltB"
                     /locus_tag="POA77_002982"
     CDS             155..1240
                     /gene="mltB"
                     /locus_tag="POA77_002982"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDI6366379.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            1385..1882
                     /gene="pncC"
                     /locus_tag="POA77_002983"
     CDS             1385..1882
                     /gene="pncC"
                     /locus_tag="POA77_002983"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDI6366380.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            1962..3023
                     /gene="recA"
                     /locus_tag="POA77_002984"
     CDS             1962..3023
                     /gene="recA"
                     /locus_tag="POA77_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI6366381.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            3266..3766
                     /gene="recX"
                     /locus_tag="POA77_002985"
     CDS             3266..3766
                     /gene="recX"
                     /locus_tag="POA77_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI6366382.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            3894..6524
                     /gene="alaS"
                     /locus_tag="POA77_002986"
     CDS             3894..6524
                     /gene="alaS"
                     /locus_tag="POA77_002986"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI6366383.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            6759..6944
                     /gene="csrA"
                     /locus_tag="POA77_002987"
     CDS             6759..6944
                     /gene="csrA"
                     /locus_tag="POA77_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDI6366384.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            7260..7352
                     /locus_tag="POA77_002988"
     tRNA            7260..7352
                     /locus_tag="POA77_002988"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7294..7296,aa:Ser,seq:gct)
     gene            7356..7432
                     /locus_tag="POA77_002989"
     tRNA            7356..7432
                     /locus_tag="POA77_002989"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7390..7392,aa:Arg,seq:acg)
     gene            7630..7706
                     /locus_tag="POA77_002990"
     tRNA            7630..7706
                     /locus_tag="POA77_002990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7664..7666,aa:Arg,seq:acg)
     gene            7770..7846
                     /locus_tag="POA77_002991"
     tRNA            7770..7846
                     /locus_tag="POA77_002991"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7804..7806,aa:Arg,seq:acg)
     gene            8045..8121
                     /locus_tag="POA77_002992"
     tRNA            8045..8121
                     /locus_tag="POA77_002992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8079..8081,aa:Arg,seq:acg)
     gene            8402..8968
                     /gene="yqaB"
                     /locus_tag="POA77_002993"
     CDS             8402..8968
                     /gene="yqaB"
                     /locus_tag="POA77_002993"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDI6366385.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            8965..9393
                     /locus_tag="POA77_002994"
     CDS             8965..9393
                     /locus_tag="POA77_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6366386.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            9466..11022
                     /gene="gshA"
                     /locus_tag="POA77_002995"
     CDS             9466..11022
                     /gene="gshA"
                     /locus_tag="POA77_002995"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI6366387.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            11172..11687
                     /gene="luxS"
                     /locus_tag="POA77_002996"
     CDS             11172..11687
                     /gene="luxS"
                     /locus_tag="POA77_002996"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI6366388.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(11751..13289)
                     /gene="emrB"
                     /locus_tag="POA77_002997"
     CDS             complement(11751..13289)
                     /gene="emrB"
                     /locus_tag="POA77_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDI6366389.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(13306..14478)
                     /gene="emrA"
                     /locus_tag="POA77_002998"
     CDS             complement(13306..14478)
                     /gene="emrA"
                     /locus_tag="POA77_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDI6366390.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(14605..15135)
                     /gene="emrR"
                     /locus_tag="POA77_002999"
     CDS             complement(14605..15135)
                     /gene="emrR"
                     /locus_tag="POA77_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDI6366391.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(15226..15561)
                     /gene="ygaH"
                     /locus_tag="POA77_003000"
     CDS             complement(15226..15561)
                     /gene="ygaH"
                     /locus_tag="POA77_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDI6366392.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(15551..16288)
                     /gene="ygaZ"
                     /locus_tag="POA77_003001"
     CDS             complement(15551..16288)
                     /gene="ygaZ"
                     /locus_tag="POA77_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDI6366393.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(16412..17596)
                     /locus_tag="POA77_003002"
     CDS             complement(16412..17596)
                     /locus_tag="POA77_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6366394.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGAAIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(17887..18879)
                     /gene="proX"
                     /locus_tag="POA77_003003"
     CDS             complement(17887..18879)
                     /gene="proX"
                     /locus_tag="POA77_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDI6366395.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(18936..20000)
                     /gene="proW"
                     /locus_tag="POA77_003004"
     CDS             complement(18936..20000)
                     /gene="proW"
                     /locus_tag="POA77_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDI6366396.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     ALNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(19993..21195)
                     /gene="proV"
                     /locus_tag="POA77_003005"
     CDS             complement(19993..21195)
                     /gene="proV"
                     /locus_tag="POA77_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDI6366397.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
BASE COUNT         5245 a         5305 c         5739 g         4912 t
ORIGIN      
        1 aaatatttta atttgaaagt ttgagtaaaa ggatagcgag gggaatgagt tgagttatgt
       61 aaagtccgta tcgggcagtg actaccgctt ccttgtgtgg ggcgtgatgc tttaccatac
      121 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
      181 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
      241 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag ccgcagcaca atgtgatgca
      301 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca aaatggtgaa
      361 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
      421 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
      481 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
      541 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
      601 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
      661 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
      721 ggagtatttc tctggcgagc tggaaacctt cctgctgatg gcgcgcgacg agcaggacga
      781 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
      841 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
      901 ggttgatgcg atcggtagcg tggcgaatta tttcaaagcg cacggctggg tgaaaggcga
      961 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
     1021 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
     1081 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
     1141 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
     1201 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
     1261 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
     1321 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgacagaggt
     1381 tgtgatgact gacagtgaac tgatgcagtt aagtgaacag gttgggcagg cgctgaaagc
     1441 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
     1501 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
     1561 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
     1621 tgaacccgtc gtggtggaaa tggtgatagg cgcactgaaa gcggctcgtg ctgattatgc
     1681 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
     1741 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
     1801 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
     1861 acaatttcta caaaacactt gatactgtat gagcatacag tataattgct tcaacagaac
     1921 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
     1981 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
     2041 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
     2101 cactggatat cgcgcttggg gcaggtggtc taccgatggg ccgtatcgtc gaaatctacg
     2161 gaccggaatc ttccggtaaa accacgctga cgttgcaggt gatcgccgca gcgcagcgtg
     2221 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
     2281 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
     2341 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
     2401 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
     2461 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
     2521 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
     2581 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
     2641 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
     2701 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
     2761 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
     2821 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
     2881 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
     2941 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
     3001 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
     3061 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cagcactggc
     3121 gtgaaaaatt actctcacct cgatcctcta acgaattggg cctgcgggaa cattctgcac
     3181 actcattgac tattaaatct gtttatcagc aaaacctggg tcgcatctgc ggcccttttg
     3241 cttttttaag ttgtaaggat atgccatgac agaatcaacg tcccgtcgcc cggcatatgc
     3301 tcgcctgttg gatcgtgcgg tacgcattct ggcggtgcgc gatcacagtg agcaagaact
     3361 gcgacgtaaa ctcgcggcac cgattatggg caaaaatggc ccagaagaga ttgatgctac
     3421 ggcagaagat tacgagcgcg ttattgcctg gtgccatgaa catggctatc tcgatgacag
     3481 ccgatttgtt gcgcgcttta tcgccagccg tagccgcaaa ggttatggac ctgcgcgtat
     3541 tcgccaggaa ctgaatcaga aaggtatttc ccgcgaagcg acagaaaaag cgatgcgtga
     3601 atgtgacatc gactggtgcg cactggcgcg cgatcaggcg acgcgaaaat atggtgaacc
     3661 tttgccaact gtcttttcag aaaaagttaa gatccagcgt tttttgctct atcgtggcta
     3721 tctgatggaa gatatccagg atatttggcg aaattttgcc gactgaacgc atacggtatt
     3781 ttacttccca gtcaagaaaa cttatcttat tcccactttt cagttaccag cccggcggtt
     3841 aagacacgct ggagctggtg gcgatatttc gttagcttga tttcaggata attatgagca
     3901 agagcaccgc tgagatccgt caggcgtttc tcgacttttt ccatagtaag ggacatcagg
     3961 tagttgccag cagctccctg gtaccccata acgacccaac tttgttgttt accaacgccg
     4021 ggatgaacca gttcaaggat gtgttccttg ggctcgacaa gcgtaattat tcccgcgcta
     4081 ccacttccca acgctgcgtg cgtgcgggtg gtaaacacaa cgacctggaa aacgtcggtt
     4141 acaccgcgcg tcaccatacc ttcttcgaaa tgctgggcaa cttcagcttc ggcgactatt
     4201 tcaaacacga tgccattcag tttgcatggg aactgctgac cagcgaaaaa tggtttgccc
     4261 tgccgaaaga gcgtctgtgg gttaccgtct atgaaagcga cgacgaagcc tacgaaatct
     4321 gggaaaaaga agtcgggatc ccgcgcgaac gtattattcg catcggcgat aacaaaggtg
     4381 cgccatacgc atctgacaac ttctggcaga tgggtgacac tggtccgtgc ggcccgtgca
     4441 ccgaaatctt ctacgatcac ggcgaccaca tttggggtgg ccctccggga agtccggaag
     4501 aagacggcga ccgctacatt gagatctgga acatcgtctt catgcagttc aaccgccagg
     4561 cagatggcac gatggaaccg ctgccgaagc cgtctgtaga taccggtatg ggtctggagc
     4621 gtattgctgc ggtgctgcaa cacgttaact ctaactatga catcgacctg ttccgcacgt
     4681 tgatccaggc ggtagcgaaa gtcactggcg cgaccgatct gagcaataaa tcgctgcgcg
     4741 taatcgctga ccacattcgt tcttgtgcgt tcctgatcgc ggatggcgta atgccgtcca
     4801 atgaaaaccg tggttatgta ctgcgtcgta tcattcgtcg cgcagtgcgt cacggcaata
     4861 tgctcggcgc gaaagaaacc ttcttctaca aactggttgg tccgctgatc gacgttatgg
     4921 gctctgcggg tgaagacctg aaacgccagc aggcgcaggt tgagcaggtg ctgaagactg
     4981 aagaagagca gtttgcccgt actctggagc gcggtctggc gttgctggat gaagagctgg
     5041 caaaactttc tggtgatacg ctggatggtg aaacggcttt ccgtctgtac gacacctatg
     5101 gcttcccggt tgacctgacg gctgatgttt gtcgtgagcg caacatcaaa gttgacgaag
     5161 ctggttttga agcagcaatg gaagagcagc gtcgtcgcgc gcgcgaagcc agcggctttg
     5221 gtgccgatta caacgcaatg atccgtgttg atagtgcatc tgaatttaaa ggctatgacc
     5281 atctggaact gaacggcaaa gtgaccgcgc tgtttgttga tggtaaagcg gttgatgcaa
     5341 tcaatgcagg ccaggaagct gtggtcgtgc tggatcaaac accattctat gcggaatccg
     5401 gcggtcaggt tggtgataaa ggcgaactga aaggcgctaa cttctccttc gcggtggaag
     5461 atacgcagaa atacggccag gcgattggtc acatcggtaa acttgctgcg ggttctctga
     5521 aagtgggcga cgctgtgcag gctgatgttg atgaggctcg tcgcgcccgt attcgtctga
     5581 atcactccgc aacgcacctg atgcacgctg cgctgcgcca ggttctgggg actcatgtat
     5641 cgcagaaagg ttcactggtt aacgacaagg tgctgcgctt cgacttctca cacaacgaag
     5701 cgatgaaacc ggaagagatt cgtgcggtcg aagacctggt gaacgcacag attcgtcgca
     5761 atttgccgat cgaaaccaac atcatggatc tcgaagcggc gaaagcgaaa ggtgcgatgg
     5821 cgctgttcgg cgagaagtat gatgagcgtg tacgcgtgct gagcatgggc gatttctcca
     5881 ccgagctgtg tggcggtact cacgccagcc gcactggtga tattggtctg ttccgcatca
     5941 tctctgaatc gggtactgct gcaggcgttc gtcgtatcga agcggtaacc ggagaaggcg
     6001 ctatcaccac cgttcatgca gacagcgatc gcttaagcga agtcgcgcat ctgctgaaag
     6061 gcgatagcaa taatctggcg gataaagtgc gctcagtact ggaacgtacg cgtcagttag
     6121 aaaaagaact acaacagctt aaagaacaag ctgccgcaca ggagagcgca aatctttcca
     6181 gtaaggcaat tgatgttaat ggtgttaagc tgttggttag cgagcttagc ggtgttgagc
     6241 cgaaaatgtt gcgtaccatg gttgacgatt taaaaaatca gctggggtcg acaattatcg
     6301 tgctggcaac ggtagccgaa ggtaaggttt ctctgattgc aggcgtatct aaggacgtca
     6361 cagatcgtgt gaaagcaggg gaactgattg gtatggtcgc tcagcaggtg ggcggcaagg
     6421 gtggtggacg tcctgacatg gcgcaagccg gtggtacgga tgctgcggcc ttacctgcag
     6481 cgttagccag tgtgaaaggc tgggtcagcg cgaaattgca ataatataag cgtcaggcaa
     6541 tgccgtggac tcgcttcacg gcattcgcat taacgctatc gacaacgata aagtcaggtt
     6601 gaagttgtgt atatcggcta aacttaggtt taacagaatg taatgccatg actgcttaca
     6661 tgtaatgtgt ttgtcattgc ttactttttg gcgttatatg atggataatg ccgggataca
     6721 gagagacccg actcttttaa tctttcaagg agcaaagaat gctgattctg actcgtcgag
     6781 ttggtgagac cctcatgatt ggggatgagg tcaccgtgac agttttaggg gtaaagggca
     6841 accaggtacg tattggcgta aatgccccga aggaagtttc tgttcaccgt gaagagatct
     6901 accagcgtat ccaggctgaa aaatcccagc agtccagtta ctaatctttc cgcgtctcat
     6961 ctttatcggt gagacgcacc ctcaaaattt ctccttcact ctatagtctt ttcgctttac
     7021 tcccgttcat tcaacttaag tctccatttt tttgcattac tactatctgt cagacctcca
     7081 tttttctgtt gataaaacac tctttttgac gtttttacag actaattgaa cgtgaagtgt
     7141 gcaaacgata aaagtgtagg aaaaattgtt tgacttataa gtctcagaaa gtaatatgtg
     7201 cgccacgcag cgacgatgag cgataaacaa gttcttcgaa gcactcgtaa gaggcgtgtg
     7261 gtgaggtggc cgagaggctg aaggcgctcc cctgctaagg gagtatgcgg tcaaaagctg
     7321 catccggggt tcgaatcccc gcctcaccgc catttgcatc cgtagctcag ctggatagag
     7381 tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc ccggatgcac catattctac
     7441 gtactttcag cgatgaaggt atggaagagg tggcggtata accgcaggca ccagggagga
     7501 taacgttgct ttagcaacgg cccgaagggc gagccgcaag gcgagtaatc ctcccggatg
     7561 taccatctct tacttgatac ggctttagta gcggtatcaa aaaatctgca gtaaagtaag
     7621 tttcccgatg catccgtagc tcagctggat agagtactcg gctacgaacc gagcggtcgg
     7681 aggttcgaat cctcccggat gcaccatctc ttacttgata tggctttagt agcggtatca
     7741 atatcagcag taaaataaat tttcccgatg catccgtagc tcagctggat agagtactcg
     7801 gctacgaacc gagcggtcgg aggttcgaat cctcccggat gcaccatatt ctccgaaatc
     7861 ttcagcaatg aaggtatcga agaggtagcg gaattaaccg caggcaccag ggatgataac
     7921 gttgctttag caacggcccg aagggcgagc cgcaaggcga gtaatcctcc cggatgcacc
     7981 atctcttact tgatatggct ttagtagcga tatcaatatc agcagtaaaa taaattttcc
     8041 cgatgcatcc gtagctcagc tggatagagt actcggctac gaaccgagcg gtcggaggtt
     8101 cgaatcctcc cggatgcacc atattctccg taaccttcag caatgaaggt atcgaagaga
     8161 tagcggtatt aaccgcaggc accagggagg ataacgttgc gtcagcaacg gcccgaaggg
     8221 cgaacgccag tgagtaatcc tcccggatgc accatcttct cccaaaaatt tccttcaaaa
     8281 atatctcatt tctaaataac tactttatcc atttcctgaa ttttcttctc cttcgccatc
     8341 agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat ttttcgactt gcgagaagac
     8401 catgtacgag cgttatgcag gtttaatttt tgatatggat ggcacaatcc tggatacgga
     8461 gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac tacggtcttc agtacgatgt
     8521 tcaggcaatg attgcgctta atggatcgcc cacctggcgt attgctcagg caattattga
     8581 gctgaatcag gccgatctcg acccgcatgc gttagcgcgt gaaaaaacag aagcagtaag
     8641 aagtatgctg ctggatagtg tcgagccgct tcctcttgtt gaagtggtga aaagttggca
     8701 tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc gccatcgctg aggcattgct
     8761 ggcgcacctg ggattacgcc gttattttga cgccgtcgtc gctgctgatc acgtcaaaca
     8821 ccataaaccc gcgccagaca catttttgtt gtgcgcgcag cgtatgggcg tgcaaccgac
     8881 gcagtgtgtg gtctttgaag atgccgattt cggtattcag gcggcccgtg cagcaggcat
     8941 ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat cgcttttctc gttgtttgcc
     9001 agtagttttc tcagcgctac actattaccc ggcaactccg aagtcgtgct ggtggcaatg
     9061 ttgctttccg ggatcagtta tccctgggtt ttagttttaa cagcaacaat gggtaatagc
     9121 cttggagggt taactaacgt tatccttggg cgtttctttc cattgcgtaa aacatcgcgc
     9181 tggcaagaga aagctactgg ctggctgaaa cgctatggtg cagtcacgct attattaagc
     9241 tggatgcccg tggttggcga tttattgtgt ctgttagcgg gatggatgcg catctcgtgg
     9301 ggaccggtaa tctttttttt gtgccttggt aaagcgttac gctatgttgc agttgcagca
     9361 gctaccgttc agggcatgat gtggtggcac taattgtagg cctgcacata tggtcaccat
     9421 tacagttatg ctaattaaaa cgattttgac aggcgggagg tcaatttgat cccggacgta
     9481 tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt taaaggggat acagcgtggg
     9541 ctggagcgcg aaactttgcg tgttaatgct gatggcacac tggcaacaac aggtcatcct
     9601 gaagcattag gttccgcact gacgcacaaa tggattacta ccgattttgc ggaagcattg
     9661 ctggaattca ttacaccagt ggatggtgat attgaacata tgttgacctt tatgcgcgat
     9721 ctgcatcgtt atacggcgcg caatatgggc gatgagcgga tgtggccgtt aagtatgcca
     9781 tgctacatcg cagaaggtca ggacatcgaa ctggcacagt acggcacttc taacaccgga
     9841 cgctttaaaa cgctgtatcg tgaagggctg aaaaatcgct acggcgcgct gatgcaaacc
     9901 atttccggcg tgcactacaa tttctctttg ccaatggcat tctggcaagc gaagtgcggt
     9961 gatatctcgg gcgctgatgc caaagagaaa atttctgcgg gctatttccg cgttatccgc
    10021 aattactatc gtttcggttg ggtcattcct tatctgtttg gtgcatctcc ggcgatttgt
    10081 tcttctttcc tgcaaggaaa accaacgtcg ctgccgtttg agaaaaccga gtgcggtatg
    10141 tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc tcggctatac caataaatcg
    10201 caaagcaatc ttggtattac cttcaacgat ctttacgagt acgtagcggg ccttaaacag
    10261 gcaatcaaaa cgccatcgga agagtacgcg aagattggta ttgagaaaga cggtaagagg
    10321 ctgcaaatca atagcaacgt gctgcagatt gaaaacgaac tgtatgcgcc gattcgtcca
    10381 aaacgcgtta cccgcagcgg cgagtcacct tctgatgcgc tgttacgtgg cggcattgaa
    10441 tatattgaag tgcgttcgct ggacatcaac ccgttctcgc cgattggtgt agatgaacag
    10501 caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc tggctgatgc accggaaatg
    10561 agcagtagcg aacttgcctg tacgcgcgtt aactggaacc gggtgatcct tgaaggtcgc
    10621 aaaccgggcc tgacgctggg tatcggctgc gaaaccgcgc agttcccgtt actgcaggtg
    10681 ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa cgctggatag cattaacggc
    10741 ggcgaagcgt atcagaaagt gtgtgatgaa ctggttgcct gcttcgataa tcccgatctg
    10801 actttctctg cccgtatctt aaggtctatg attgatactg gtattggcgg aacaggcaaa
    10861 gcgtttgcag aagcctaccg taatctgctg cgtgaagagc cgctggaaat tctgcgcgaa
    10921 gaggattttg tagccgagcg cgaggcatct gaacgccgtc agcaggaaat ggaaaccgct
    10981 gataccgaac cgtttgcggt gtggctggaa aaacacgcct gacagaaaag aaaaaggcca
    11041 ctcgtgagtg gccaaaattt catctctgaa ttcagggatg atgataacaa atgcgcgtct
    11101 ttcatatact cagactcgcc tgggaagaaa gagttcagaa aattttaaaa aaaattaccg
    11161 gaggtggcta aatgccgttg ttagatagct tcacagtcga tcatacccgg atggaagcgc
    11221 ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg cgacgcaatc accgtgttcg
    11281 atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga aagagggatc cataccctgg
    11341 agcacctgtt tgctggtttt atgcgtaacc atcttaacgg taatggtgta gagattatcg
    11401 atatctcgcc aatgggctgc cgcaccggtt tttatatgag tctgattggt acgccagatg
    11461 agcagcgtgt tgctgatgcc tggaaagcgg caatggaaga cgtgctgaaa gtgcaggatc
    11521 agaatcagat cccggaactg aacgtctacc agtgtggcac ttaccagatg cactcgttgc
    11581 aggaagcgca ggatattgcg cgtagcattc tggaacgtga cgtacgcatc aacagcaacg
    11641 aagaactggc gctgccggaa gagaagttgc aggaactgca catttagtca gtaaactatc
    11701 ttcacaatta attgaaaaaa gccagttcaa atgaactggc ttagttgtac ttagtgcgca
    11761 ccgcctccgc cgccacctgc gccaaatggc ggtttagcaa accacaccag ccccagcagg
    11821 acgaggaata tcccggctga catccagaag atctcattgg cggaaataat cagcccctgg
    11881 ttggtgatct gctgggcaat ccagccggag gcctgctgtt gcgtcatccc aagcccttcc
    11941 agttgactgt acatcgcctg ggcgtttggg ttaaacgggt ttaccgactc ggttaactgt
    12001 gcgtggtgca tcgactcgcg gttggtccac atggtggtgg tgatggacgt gccgatagac
    12061 cccgccagcg tacgcgtaaa gttggagagg ctcgatgccg ccgccagtcg ttccggtggc
    12121 aaaccagaca gcgtaatggt ggtcagcggc ataaagaagc aggccaccgc aaacccctgg
    12181 ataaactgcg gccaggccga cgcgccaaaa tccatacctg gttcaaaggt ataggcacgc
    12241 cagtagaagc agacggcata cataataaag ctgaaggtta ccagccgccg catatccagt
    12301 ttatgcgcga agcggccgat aatcggcgac aggatcaccg gaataatccc taccggcgca
    12361 gaggccagac ctgcccacgt tgccgtgtaa ccgtagacct cctgcaacaa ctgcggcagc
    12421 agaacaatcg cgccgaagta gagcatatac gcgaggctga tacacaagca gccgatggtg
    12481 aagttgcgcg acttaaacaa cgacagatcg actatcgggt tatcgtcggt cagctcccag
    12541 acaattagga agcagatagc caccactgcc accacggtaa gaatgataat ttcctgtgat
    12601 gagaaccagt ccagctcttt accgcggtcg agcataatct gcaggctgcc gataccaata
    12661 accagcagtg ccagccccac ggcatcaatc cgccgccgtt cggtgcgggt ttcacgtccg
    12721 cgcagagttt gcagtgtcat caacaccacc gccacgccaa tcggcacgtt gatgaagaat
    12781 atccagcccc agtggtaatt atcgctgata taaccgccga ggatcgggcc gcaaattggc
    12841 gcgacaatca ccgtcatcga ccacaacgcc agcgcgatcg agcgtttggc tggcgggtag
    12901 ttattcagca atagactttg cgaaagcggg atcaacggcc cggcgacaat cccctgaatc
    12961 acgcggaaga agatcagcat attcaggctg ctggagacac cacacgccca cgacgcaata
    13021 gcaaaggcga tggtggacca aaggaacagt ttcacttccc cgacgcgctt tgccagccag
    13081 ccggtaagcg ggatcgagat ggcattcgcc accccgaaag aagtgattac ccacgttccc
    13141 tggctgagcg atgagcccag attcccggcg atagtgggga tcgccacgtt agcaatggtg
    13201 gagtccagca cctgcatgaa tgtcgccagt gacagcgcaa tcgtcataat gaccagttgc
    13261 gcgccttcca gcggtttttg ctgttgcatc acacgcacct ctggattagc cagcgttagc
    13321 ttttacgata tcgtcgatca gtttattgac aggtgccagg ctgatttcac gcgcggtact
    13381 taccgcgacc ggtgttgaac gtactttatt tgccagtacc tgaccgtcac ggttagtggt
    13441 attgacgctc accagcgtgg acaaaccaat gcgcagcggg tattgctcca gctgtttctg
    13501 gtccagttcg atacgcacag gtagacgctg aacgactttg atccagttac cggtcgcatt
    13561 ttgcgccgga agcagtgaga atgcgctacc tgtgcccata tccagaccaa ccactttacc
    13621 ggtgtatttc acatcatcgc cgtaaatatc ggtggtgata gtgaccggct gaccgatacg
    13681 catattggca atctgcgtct ctttaaagtt ggcatccacc cacatattgg tggctggaac
    13741 gaccgccatc agcggcgtcg ttgggctaat ttgcgcccca ggctgtaccg cgcggcggga
    13801 gacataaccg gtcatcggac tgacaatacg ggtacgctcc agcgccagcc aggcgttacg
    13861 tacttcggtg gcagcctgtt gcacggcagg ctgatcttcc agtttagtcc ctagaatcat
    13921 cgcctgattg gcattgtatt gttgaatagc gacgtccagt tgcgcctggg cactggtgac
    13981 ggcgtcgcgg gcgtgttgca gctcttcgcg accaatcagg ttggcattgc ccagcggcac
    14041 acggcggttg tagtcgcttt gtgctttcgc gagggcgatt ttttgcacct caatattcgc
    14101 ctgcaactgt ttgctgttaa tcatcagctg gtgggtttgg cgaacgctgg aagccagtgc
    14161 agttttggct ttttcaaacg cctggcgagc atctgtcggg tcgagagtga ccagaacatc
    14221 gccttctttt acaaaatcgg tgttatcggc ccagactttc gtcacgctgc cagacacctg
    14281 agacataatt tgaatctgat tccctgccac gtatgcgtca tcggtttctt cgaaatgacg
    14341 cagtaccaaa aaccaataaa tccctatcgc tacggcaata attataaaga gcaaggtgag
    14401 aaggaggagc agacgcttac gtttgccgct cttctttacc ggttgctgcg gggtttgagt
    14461 ctccgcattt gcgctcatat tgttctccac gatcttctta actcatcggc tgagccgacc
    14521 tgcttgccaa aaaggccagc atgttgcgat gctggccggt cattttttct tatataaatc
    14581 tggatttttg agcgagatga cgcgttagct catcgcttcg agaaccacac cgtcttgttc
    14641 catctggtcg agacgggaga gcaatttgcg ggtgatttgc tcgagctgat ctttttctgt
    14701 tgtgctgagc gcggaccaga gttgatgcag gcagttatgc tgcggtggta aaacctcgcg
    14761 caaaaactcg tgaccttttt ccgttaattg cagatgcagg cagcggcgat cgttatcgct
    14821 ttcacgacgt tcgatccaac cgcgtttttc cagttcatcg gcaatacgcg tcgcgttggt
    14881 acgggatgat ccaagagcac aacttaattc agaaggctga atactgtggt tttcctgaga
    14941 ctccagcgta atcaacgcca taaacaacgt ctcgttaatc ccctgagcct tcagcatttt
    15001 attgcggttc tccagcagct tgctttgcat gtgcatgcaa agacgagtca gaaggatctc
    15061 ctgataagga aaatcttcgt ggcggctggc gcgaaatttt agcatttgtt caatgggcgt
    15121 aaacgaacta tccatttggg tatgacctca ttaatttcag ccgatatagt aacgacagtg
    15181 acaaataaag taaatgtatt gttttagaaa aatgattctt gtgggttata taatcgccat
    15241 cactttccag gcgagcccat aggccagcgc actaagcagt gttgggatga taatgctgcg
    15301 tgttttatag aaactggcac ccagtaccgc gaagccgacc agcgtgggca cgaaacggcg
    15361 tgtatcgtgc atcacttctg gtgcggtaga gacaaccagc agagcgcata tcgaggcgat
    15421 gccaatggtg tcgagcaaaa tacctaccgc gccacgtttg gttgggcggg cattacccac
    15481 acgcaggcgc agcggcaaat agcggaagca ataattcgcc acgccaacca gtaacccaag
    15541 cagcagaacc tcatagctca tcgggcgctc cttgccagaa tgcctggatt aacgcagtga
    15601 ggcagccaca gacaatgcct gccagaatgg cgacgggaat agaaaatagc gttacgcctg
    15661 caagggcacc aactaacgct gcggtaacgc aaagagattg tttgcgctgg aaagaggcga
    15721 gcaggaaact cataaagagt gccggaagca taaaacctaa tgcagcttca acggcgggat
    15781 aaccttgcag caagccgctg ccggagaatg cccctattac cgtaccaaat acccacgatg
    15841 accatgaact gaaggcaatg ccgatcatcc agttctcgct ccagcggcga ttattgcgta
    15901 ccagttttgc ggttgcggcg gcaaaaacct catccgtcag gccaaatgcc cacagggcgg
    15961 ttttcgattt ttgcagacgc tgaataatac ggctacgcag tgacgggcca tacaacacat
    16021 ggcgaacatc cattgccatg acggtcagtg cagcaaccca caaactactc ccggctgcca
    16081 gcatcgcggt aatgacgaac tggctcgcgc ctgcataaat gatgcaggag aaaaaaacgc
    16141 tttcgagagg agagaatccc agacgggtcg cattcagacc gaacgcaaag gccaccggaa
    16201 tataactaat aacaatcggt aaactgtctt tgcatccttc catgaaggtc gccgaaccag
    16261 gagcaggctg tggagtaggg ctttccatag agtgtacgct taacgattgt taatattata
    16321 aatagactga atgaatatct taaccttatc agactgatgg gcttcttaac acccttataa
    16381 gtgtaaagcc acgaaaacgg ttgctgattg cttaatttgc ggcttcctga cgatgaaaac
    16441 ctcgccacca gaccagtaag ttaaccaggg caatcgccgc gccagccaga caaacgccag
    16501 cccaaccgcc atgttgccag gctgaggctg aaattagcga accggcggca ccgccaataa
    16561 agtagctggt catgtaacct gcggtcaggc gattacgcgc atcagggtgt attcgataaa
    16621 ttaccgtctg gttagtgata tgcacgccct gtacggtgag atccagcacc aggatgccga
    16681 taatcagcgc cagtacggaa gtgtgaccaa accagatcgc cagccatgaa agtaatagca
    16741 gcagcagacc gaaagttgtg gtgtggtgcg atttaccctt atcggcaaaa ccgcccgccg
    16801 gacgagcgcc caacgctccg gcagctcccg caagtccaaa cagaccaatg acaccatcgc
    16861 tgtagttaaa aggtggagcg gcaagcaaaa aggccattga ggtccagaga atgctgaagt
    16921 tggcaaaggt caggcagccc agcaatgcgc gggtgcgcag gatcttatcg ctgataaaca
    16981 tgctgaaaac ggaacctaac aactgtgggt agttgaggtg ggtttctgat ttcatttgtg
    17041 gcagaccacg ccataatgcc agcgccatca gtgccattaa caccgaggca acccaaaaga
    17101 cggtgcgcca gccgccgaga ttcgccagca atccggcaac ggtccgtgcg agtaagatcc
    17161 ccaacagcag accgctcata atagtgccaa ccactttgcc gcgtttgtcc ggtgaagcca
    17221 gcgtcgctgc cagcggaacc agaatttgtg ccacgactga gaataaaccg gttaatgccg
    17281 taccgaggat catcatcgcc agtgactgac tgctggcagt gattaacata ccgcccgctg
    17341 ccagtaaggt catcgagaca atcaggcggc ggcgttcaaa catatcaccg aggggaacaa
    17401 gaaacagtag acctgcggcg tagcccaact gcgcggcggt aacaataaag cctgccgaac
    17461 tggcggaaag ggaaaagtta cgcgcgatgg tgtcgagcaa tggctgggcg taatagttac
    17521 tggctaccgc cagaccggtg gcgatagaca tcagcacgat cagcgccggg ctaagctcat
    17581 gattaggttt agtcattatt tttgttcatc aggtgatgat taagctggaa atcaataata
    17641 gcggatgttg atgaatatgg gatgaaagat agattgttga aatgtgcgat gtggtttgta
    17701 ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaacggc ggatgcggcg
    17761 aaaacgcctt atccgccata cgaatgagtg tattttgtag gcatgataag acgcgtcagc
    17821 gtcgcatcag gcattgtgta cagagcggcg gatgaggcaa aaacgcctta tccgcctgaa
    17881 taaaaattac ttctgcgctg ccagcgcctc attcacccag ccatcgaact gctgctggtg
    17941 ggctttgatc cagccatcaa cgtggccctg aatatcgcct tctgaggctt tgccgtcatg
    18001 cataatggcg ttctgggcgt taatatcggc cactggcaac tgcataatgg caaacagttt
    18061 ggctgccgcc ggatttttct cggtccaggc tttgttggca acgatatgca tggtgctgac
    18121 cgggaagccg taattcgcgc cattcggcag tttggtatcg gcatttttat cgcccggcag
    18181 tgcggagaac ggcacctgca accagaccac atctttccct ggcttcagct cattactcac
    18241 ccagtacggc gtccaggtgt aataaaacac cggtttgccc tctttgtagc gactgatggt
    18301 gtcggccatc atcgctgcgt agttcccctg attatgcgtc acggtgttgg tcagttcata
    18361 cgcggcaagc tggtggttga tggcaccttc gcagccccag ccagggttac aaccggttaa
    18421 atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg atcttcggat ctttcagttg
    18481 tgcgatgttg gtgattttat actggtcggc ggttttctta tcgatcaggt aaccctgtgc
    18541 cgcgccgtta acaaataccc cttcacgata aaatttctta tcgccaccgg cagcttcgta
    18601 catgttgtca tgcagcggcg tccagttcac ggcggtgaag gttgcatcgc cggaagcaag
    18661 cgaggtgtag ccaacgttgt aatctacttc gctgggttta ttgacggtat aacctaattt
    18721 ctccagcgca cgactgacca gcagtgtctg gaaggtttct tcggtgatgg tgctttgaac
    18781 tggattaact gtaatgcctt tgcccggcag gtcggcagca aaagtttgtg tagagataag
    18841 cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt gttattcctt tttttggcag
    18901 ggtgatatgc ccggcgttac cgggcaagtg cagagttact taatgaatgg gcgggtcagc
    18961 agaccgacag ggccagtggt gtaccagcga cggttgccgc gactgcgtga gtcgcgccca
    19021 acggcctgcg tcaggcggtc gaggataatg gcgaggatca caatcccgac gccgccaacg
    19081 gtggcaagcc ccatatccag acgaccgata ccgcgaagta ccatctgacc cagcccgccg
    19141 acggcaatca tcgaggcaat gaccaccata gaaagggcca gcatcagcgt ctggttaacg
    19201 cccgccataa tggtcggcat cgccagcggt aactgaactt tgaacagcat ctggcgcggg
    19261 ctggcaccga atgagcgcga ggcttcaatc agatccgccg gaacctggtt aatccccaga
    19321 atggtcagac ggataatcgg cggcagagca aagatgatcg tcaccaccac gcccggcacg
    19381 ttaccgatac caaatagcat gacgattggc accagataaa caaacgctgg cgtggtctgc
    19441 atggcatcaa gcagtggacg aataattttc gccgctcgcg gacttctcgc cagccagatc
    19501 cccagcggca aaccaatgac gatacagaac agcagggcgg ttaacaccag cgccagggta
    19561 accatggcct gcgaccaggc accgattgcg ccgatggcaa tcagcgaaac cagcgtcgcc
    19621 acgcccattc cgaccccgga aatctgccag gcgatgagag cgaaaacgat aatcgccacc
    19681 ggtgcgggca tacccagcag caattgctgg aaaccgttga gaatgtaatc aaccggaacg
    19741 cgcacgccct ggaagacggg acggaaatgg gtaacaaccc agtcgatccc ttcagtgacc
    19801 caactgtcga gcgggatcag cgttttatgg aacggatcga gaatattaaa atgctcgacg
    19861 tttagcgcag gcgtactggt cagccagtca gcaccaccgc cgtcagtcgg tgcagtcgcc
    19921 ggtgtacccc aggcgtctgc ggattgcacg gcactgtccg ccgctggcgt ggtatcccac
    19981 ggattatttt gatcagccat tatttacccc ctcacgatct aaagcgcgca gcagcattcc
    20041 tttcgaaatg atgccgacat actgttggtc ctcgtcgacc acgggcaccg cacagggtgc
    20101 ctgtccgaca tgagagagca actcgctaag aggcgtttgt gcatcgactg ctaacggcgc
    20161 atcaatcagc gccgcatcaa gaccttgctg ctgcgttaac gcggttttaa gcgaatcgat
    20221 ggaaactgcg ccgacaaact tattaccgcg ttcgataacg tagccatatt cgcgatcttc
    20281 atcctgcaat aatttcagtg ccgaacgtgg gccgaagcca ggggttttac gaattaagcc
    20341 attcggtgtc cggcgggcaa tatctttcgc actgaatacc tgactaatat caacgccacg
    20401 gaagaaggta cggacataat cattcgccgg attattgaga atttcatccg gtgtgccgac
    20461 ctgtaccact tcaccatttt gcataatggc aattcggtcg ccaatacgca tagcctcatc
    20521 gagatcgtgg gaaataaaga caatggtgcg ctgatgtttc gcctgtaatt ttaccagttc
    20581 atcctgcatc tcggtgcgaa ttaatggatc gagcgccgag aaggcttcgt ccattaataa
    20641 tatatccgga ttaatcgcta acgcgcgggc taatcccaca cgttgacgca tcccgccaga
    20701 gagttcatcc gggtagctgt gggcataatt ttccagcccg acctgacgca gtgcatcaag
    20761 ggctttttcc cggcgttctt cggcattaat tccggccaat tccataccga acgcagtatt
    20821 gtccagcacg gtcatatgcg gcattaaggc aaaggactgg aagaccatcg caatcttttt
    20881 tctgcgcacc tcacggagtt cggcatcgga tattttggca atatccacac catcaatcag
    20941 cacttgcccg cgggtgggtt caatcaggcg attgagaagg cgtaccattg tggatttacc
    21001 cgagccggat aatcccatga tgacaaatat ctcgccttct tcaatggcca gactggcgtc
    21061 ttttacgcca agcgatagcc cagttttttc cagaatttgt tctttcgaaa gtccttgttc
    21121 gatatatttg aacgctcgct gtggatgctc gccaaatatt ttataaagat ttttaatttc
    21181 taatttaatt gccatgcaat a
//
LOCUS       DAMZII010000083        21181 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZII010000083 DAMZII010000000
VERSION     DAMZII010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.083"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(456..1703)
                     /locus_tag="POA77_003006"
     CDS             complement(456..1703)
                     /locus_tag="POA77_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDI6366398.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFLSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1775..2689)
                     /locus_tag="POA77_003007"
     CDS             complement(1775..2689)
                     /locus_tag="POA77_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDI6366399.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            2905..4338
                     /gene="cscA"
                     /locus_tag="POA77_003008"
     CDS             2905..4338
                     /gene="cscA"
                     /locus_tag="POA77_003008"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI6366400.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4346..5404)
                     /locus_tag="POA77_003009"
     CDS             complement(4346..5404)
                     /locus_tag="POA77_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6366401.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLGAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            5572..5790
                     /locus_tag="POA77_003010"
                     /pseudo
     CDS             5572..5790
                     /locus_tag="POA77_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            5808..7136
                     /gene="dsdA"
                     /locus_tag="POA77_003011"
     CDS             5808..7136
                     /gene="dsdA"
                     /locus_tag="POA77_003011"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDI6366402.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7244..8782)
                     /gene="emrY"
                     /locus_tag="POA77_003012"
     CDS             complement(7244..8782)
                     /gene="emrY"
                     /locus_tag="POA77_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDI6366403.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(8782..9945)
                     /gene="emrK"
                     /locus_tag="POA77_003013"
     CDS             complement(8782..9945)
                     /gene="emrK"
                     /locus_tag="POA77_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDI6366404.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            10361..10975
                     /gene="evgA"
                     /locus_tag="POA77_003014"
     CDS             10361..10975
                     /gene="evgA"
                     /locus_tag="POA77_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDI6366405.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            10980..14573
                     /gene="evgS"
                     /locus_tag="POA77_003015"
     CDS             10980..14573
                     /gene="evgS"
                     /locus_tag="POA77_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDI6366406.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(14629..15774)
                     /gene="yfdE"
                     /locus_tag="POA77_003016"
     CDS             complement(14629..15774)
                     /gene="yfdE"
                     /locus_tag="POA77_003016"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDI6366407.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDIRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(15848..16792)
                     /gene="yfdV"
                     /locus_tag="POA77_003017"
     CDS             complement(15848..16792)
                     /gene="yfdV"
                     /locus_tag="POA77_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDI6366408.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(16862..18556)
                     /gene="oxc"
                     /locus_tag="POA77_003018"
     CDS             complement(16862..18556)
                     /gene="oxc"
                     /locus_tag="POA77_003018"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDI6366409.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(18610..19860)
                     /gene="frc"
                     /locus_tag="POA77_003019"
     CDS             complement(18610..19860)
                     /gene="frc"
                     /locus_tag="POA77_003019"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDI6366410.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(20373..21005)
                     /locus_tag="POA77_003020"
     CDS             complement(20373..21005)
                     /locus_tag="POA77_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDI6366411.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         6105 a         4919 c         4552 g         5605 t
ORIGIN      
        1 ctgcagggga caccagatac ccttcaaacg aaatctacct tcaccccgta aaagataggt
       61 ttggcagcac acttgcctta tatctactca tttttactgc aacaggttga aatctcagca
      121 ctgtcagaaa gcgctgatga ctaaacagcc ctgggccggg cgatgtaacc atcacacaga
      181 atcctgatag cgaaatatgg cgtgactcga tacttcactc cgcaatgcat tccttgatga
      241 attcgcagga ccgtgataca cgggacaggt cgctgaatga cgacaatgtc ctggaaatca
      301 gcgaaccgcg catctgaagt acatttgagc gactgtacca gaacatgaat gaggcgtttg
      361 gattaggtga ttattagcag ggctaagcac tttagtatta ttattttccg gttgagggat
      421 agggagatat cgacaacaac cggaaaagtt tacgtctata ttgctgaagg tacaggcgtt
      481 tccataacta tttgctcgcg ttttttactc aagaagaaaa tgccaaatag caacatcagg
      541 cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc aaagagtatc
      601 ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa accaatcaga
      661 aagatcgtcg acgacaggcg cttatcaaaa tttgccacgc tgtatttgaa gacggatatg
      721 acacaaagtg gaacctcaat agcatgtaac agcttcacta atgaaataat ccaggggtta
      781 acgaacagcg cgcaggaaag gatacgcaac gccataatca caacaccgat aagtaatgca
      841 ttttttggcc ctacccgatt cacaaagaaa ggaataatcg ccatgcatag cgcttcgagt
      901 accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg tgattcgaat
      961 aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa agaccacgtc
     1021 cccacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc gataaaatcc
     1081 tcttttttta cccctcccgc atccgccgct acgcactggt gatccttatc tttaaaacac
     1141 atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt gatatgggga
     1201 ctgatactta aaaatatgcc ggcaaagaac gcgccaatag catagccaaa agatccccag
     1261 gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt gaagctgtca
     1321 agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag aattagacct
     1381 acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg tccggtcaag
     1441 accaggatga aactcataca ccagatgagc ggtttcttca gaccgagttt atcctgaacg
     1501 atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata aagtgtacct
     1561 aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa cgaccaccac
     1621 agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata gtacgcattt
     1681 ctgaatggaa tattcagtgc cataattacc tgcctgtcgt taaaaaattc acgtcctatt
     1741 tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt cgacatggca
     1801 gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga gcgagatcga
     1861 taattcgtcg catttctctc tcatctgtag ataatcccgt agaggacaga cctgtgagta
     1921 acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattaaca gacattccag
     1981 caaaatggtg aacttgtcct cgataacaga ccaccacacc ttctgcacct ttagtcacca
     2041 acagcatggc gatctcatac tcttttgcca gggcgcatat atcccgatcg ttctgtgttt
     2101 ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc agttgtagcg
     2161 cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca cgaatattag
     2221 gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg
     2281 tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg ccatgtcgcc
     2341 agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa
     2401 atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg tgccattcat
     2461 cttgcttcag atacgtgata tcgactccct cagttagcag cgttctttgc attaacgcac
     2521 caaaaggatc atcccccacc cgacctataa acccacttgt tccgcctaat ctggcgattc
     2581 ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg tctgattctg
     2641 gcaagagatc tacgaccgca tcccctaaaa cccatacttt ggctgacatt tttttccctt
     2701 aaattcatct gagttacgca tagtgataaa cctctttttc gcaaaatcgt catggattta
     2761 ctaaaacatg catattcgat cacaaaacgt catagttaac gttaacattt gtgatattca
     2821 tcgcatttat gaaagtaagg gactttattt ttataaaagt taacgttaac aattcaccaa
     2881 atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgcc
     2941 ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcct
     3001 cctgccgggt ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg
     3061 ttttatcaac atcatccgat gagcgaacac tgggggccaa tgcactgggg acatgccacc
     3121 agcgacgata tgatccactg gcagcatgag cctattgcgt tagcgccagg agacgataat
     3181 gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc
     3241 tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa
     3301 tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca
     3361 ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg
     3421 atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt
     3481 tcgttgcgtg aatggacctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat
     3541 atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg
     3601 cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc
     3661 ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga acttgataac
     3721 gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc
     3781 ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc
     3841 atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggttcac
     3901 gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa
     3961 tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac
     4021 agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac
     4081 caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga cggctaccgt
     4141 agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc
     4201 gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca
     4261 gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca
     4321 ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga
     4381 tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga
     4441 taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa
     4501 ccagattatc aaagcccatt acggcgacat cctgcgggat tcgtaccccc ttcgccagaa
     4561 gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg
     4621 gtttgcccga tttgaagtgg tcattgagta aacttgcgag atcggtgtag tgatcatcac
     4681 ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaggcct
     4741 gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata
     4801 acggttgacg atagcccgcc ccgagcaaat gctgtgttga ttcatattgt gcagtgtaat
     4861 catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat
     4921 tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa
     4981 tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa
     5041 aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca
     5101 gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg
     5161 gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct
     5221 ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca
     5281 cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca
     5341 tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat
     5401 ccaacgccct atcacctgac acagcaatac aataaaaaat aacaataatt cccggacaat
     5461 tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag taagtaaaac
     5521 atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgccg
     5581 ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc
     5641 tgcacgatcg ttacggactc gcttttctgg ctagtgaagc aatattgcgg cgctacgctc
     5701 aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg
     5761 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
     5821 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa
     5881 ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga
     5941 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa
     6001 aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc
     6061 cagctatgca aaaacggctg gaaaaagaat atcagcaacc gatcagcggg caactgttac
     6121 tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg
     6181 aagtcctggc acacgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg
     6241 actacagcaa actgctttct ccggagttta aacagttctt tagccaatac agcattgctg
     6301 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
     6361 aggtgacagt tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
     6421 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta
     6481 aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt
     6541 tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta
     6601 tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
     6661 gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg
     6721 aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt
     6781 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
     6841 catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg
     6901 atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac
     6961 cttcagcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac
     7021 agatgcacgg tttcagcgca gaacaattgc gtaatgccac tcatctggtg tgggcgacgg
     7081 gaggtggaat ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac
     7141 gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact
     7201 taagatcccc ggtcttaccc gctataaccc cctcctttta tcatcaccca atgcctttcg
     7261 ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg
     7321 ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa
     7381 tttcattaag aactcctgaa agcgaaccat aatatttatc cataatttgt gacgatgaat
     7441 taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc
     7501 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa
     7561 aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg
     7621 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga
     7681 tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
     7741 acatcaaaaa actaaatgtc actaacaacc gcatgtctat tttgttgcca taacgtccta
     7801 tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt ccggcccata
     7861 tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc gctccagagt
     7921 aaaataaata cgcgcatgtg atactcacaa taccaatggt gaagttacgg gacttaaaca
     7981 aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa gagatcagag
     8041 aaataactga tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc
     8101 gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcctg
     8161 gtagattcat tttgaccggt gaagtttcag tttctcttcc tttaagtaag gttaagcata
     8221 atgtcaggac gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt
     8281 tatcgcaaat ataaccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg
     8341 accataatgc cagagcaaat gttctttttt ctggtggata attccttaat aataaactct
     8401 gtgacagtgg aattaacggc cccgccatta atccctgaac gactctaaaa aatatcagca
     8461 catcaagatt ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa
     8521 gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca gggatcgcaa
     8581 tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca
     8641 gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa
     8701 acgtcgctaa tgacaatgca atagtaacgc accataacgt cccaccggtt aatggtgccg
     8761 gagtcgattt agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc
     8821 tttttctatc ggactggtat cgataactaa agccttactg gtataagccg gcatggacgt
     8881 cacggttgaa gccagatcag gcatctcagc aatgtcttcg ttcttcgtat caatagttgc
     8941 agtcatcgat aaaccaatgc gtaaggggtg ttccatgagt tcttttggat caagagaaac
     9001 ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa
     9061 taaggagaac gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac
     9121 attttcacca taaagatcgc tgataatatt gaccgattga ccaatccgta catccgtaag
     9181 ttgtgtttct ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa
     9241 cgattgtccg gggctcactg tttcgccgac ctgaacactt cgctgggcaa tatagccggt
     9301 aaccggactc ttaatatccg tacgtttaag cgccaaccag gcttctttag ttgcatccgc
     9361 cgcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc
     9421 tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg tatctttggt
     9481 atgctccagc gtttcttttg aaataacccc ctgcttcgct aacggcactc gacggttata
     9541 atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt
     9601 atcctgtaag tatagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt
     9661 attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac cttgtcgaac
     9721 gtagttcgta tctttatgat taacgacagt gacactacct gagacttgtg cagaaattgg
     9781 atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga
     9841 ccaataggca taggcacctg aaaacgcaat aaataaaact accgccaata aagaaaagta
     9901 ttttcttctg ttagaatgtt ttttatttga attaatctgt tccactatta tctctcattt
     9961 ctcatagatg aaatttatga attgaatgaa aagagcattc attcttcata tcaaacacag
    10021 tgcatgtgtt gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa
    10081 aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt
    10141 gagaagactt atagtgctaa cttgaaacta taaatcatcg gtataatccc tgattttatt
    10201 gttgacattt catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga
    10261 agctcagatt ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta
    10321 cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
    10381 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
    10441 cttagcggag ttgactgaag ggggaagcgc cgttcagcgg gtggaaacac ttaagcctga
    10501 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
    10561 gaggaagcgc caatatagcg gaattattat tattgtctcc gctaaaaatg accattttta
    10621 cgggaaacat tgtgctgatg ctggcgctaa tggattcgtg agtaaaaaag aaggcatgaa
    10681 caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
    10741 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
    10801 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgctgaaaa
    10861 aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga
    10921 atgtaaatca ctgatggatc tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca
    10981 tgaagttttt accctatttt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
    11041 cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
    11101 cagtacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg
    11161 cagtacacaa gtcccaaacg gctaccttgt tgcataccga ttcgcagcaa caggttcgtg
    11221 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
    11281 gggaatacgc cgatcatcaa aaagcaatgg acgcgcttgc agaaggtgaa gtcgatatag
    11341 tgttatcaca tttagttact tcgccgcctc ttaatagtga cattgctgca accaaaccat
    11401 tgataattac ctttccggcg ctggtaacca cccttcacga ctcaatgcga ccgcttacct
    11461 caccaaaacc agtaaatatt gctcgggtag caaattaccc cccagacgag gtaattcatc
    11521 aatcgtttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
    11581 tatcagccgg acagaatgat tactttattg gtagtaacat cattaccagc agtatgattt
    11641 cccgctattt cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata
    11701 attttttctt gaccagaaaa gaatccgtca ttcttaatga agtactcaat agatttgttg
    11761 atgctttaac aaatgaagtt cgctatgaag tttcacaaaa ttggcttgat acaggaaacc
    11821 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcaac
    11881 atcccgattt aaaggtgctg gaaaatcctt actctcctcc ctattctatg acggatgaaa
    11941 atggctcggt tcggggcgtt atgggggata ttcttaatat tattaccttg caaacaggtt
    12001 taaatttttc tccgatcacc gtttcacaca atatccatgc tggaacacag cttagccccg
    12061 gaggatggga tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgttttat
    12121 ttgctgaagc cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
    12181 aacaaacatt aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc
    12241 aattaaaaga gatgtatccg gaggttgaat ggatacaggt cgataatgcc agcgctgcat
    12301 ttcacaaggt taaggaaggt gaacttgatg ctctggtcgc gacacagtta aattcgcgtt
    12361 acatgatcga tcattactat cctaatgaac tttatcattt tcttattcct ggcgttccga
    12421 atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
    12481 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa
    12541 tgcccaatgt gaccattgac acatgggacc tatatagcga gcaattttat attgttacga
    12601 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
    12661 ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac
    12721 tctcggattc cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
    12781 ataatagtgc ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat
    12841 tagaaaacag tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag
    12901 aaacgaaaga aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga
    12961 aaagatgcat taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat
    13021 atatttgtgg ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag
    13081 aaaaaaataa agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc
    13141 acgaaataag aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg
    13201 gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
    13261 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
    13321 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg
    13381 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc
    13441 tggttaagat cgaccctcag gcgtttaagc aggtcttatc aaatttactg agcaatgctc
    13501 tcaaatttac caccgagggg gcagtaaaaa ttacgacctc cctggttcac attgatgaca
    13561 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
    13621 aacaacaact ctttaaacgc tacagccaaa caagtgcagg tcgccagcaa acaggatctg
    13681 gcttaggctt aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttgtcattag
    13741 aaagtcatcc aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc
    13801 aagtggcgac tgtcgaggca aaagcagaac accccatcac actacctgaa aagttgagca
    13861 tattaatcgc ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat
    13921 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc
    13981 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
    14041 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
    14101 aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt
    14161 tgaccctgga tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac
    14221 ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa tacggcgaac gatctacaac
    14281 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
    14341 ttcaagcact agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
    14401 gtgcggctaa catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa
    14461 cacctgtttc agatgacagt aagcctgaaa ttcttcagtt gctgaactct gtaaaagaac
    14521 acattgcaga gctggaccag gagattgctg ttttctgtca gaaaaatgac taaatagcgg
    14581 ctcccacaat gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
    14641 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
    14701 gtccgcgcag ccgctgattt ttatcggatt acccggcatc attattcccc cggcttcaat
    14761 caacatattt ctcgcctgag tttgtggcaa tttaatggcc tcagccacac ttaataacgg
    14821 cgcgacgggt acaccaactt catgtattct ggctaaccaa acttcagctg cctgcgtttt
    14881 taacgtccgc tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct
    14941 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
    15001 aagcttgtca ttaccacaac aaatcgtaat cggcttatcc tgagtattga aaacatcaaa
    15061 aggtgccatg tagggatggc gatttcccag acgttgtggt gacttcccag ttgcgatata
    15121 tgccatcaga ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg
    15181 cgcccctctc tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata
    15241 gacaccgccg catagatccg caagagaggt accaacgcgc actggcggag catcaggata
    15301 tcctgtttcc atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc
    15361 ttttagcgga ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat
    15421 ttcttgaagc gtttcccatg aaaaccccag tttttccatt gtacctgggc gaaaattctc
    15481 agctaataca tcagcttgtt tgagcatatt tataaatata cttttatcgt gatcattctt
    15541 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
    15601 atccacatag ggaccaaatg tgcggatatc atcaccatga cccggcggct caactttaat
    15661 tacccttgcg cccatattac aaagaagttg agttccgaaa ggtccattaa ggacatgtgt
    15721 catatcgata actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt
    15781 atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
    15841 tgattaatta tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc
    15901 ccagtacgct cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg
    15961 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat
    16021 gttcgctgtt caaatgacat gccataccaa cgagaagcag tgccagtggc atcagaatca
    16081 gcttcaggaa ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
    16141 tcaaccccgc agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg
    16201 ggtcccatgc tgccggaatt tttaccccaa ccaacaccag gatcgttgcc agaacaggtg
    16261 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
    16321 catccgctcc tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
    16381 caataataga aataattgct accactaaac ctgttgatac ggaatcacca taaataggat
    16441 cgagaactgc aaaccccagg aatccaatgg taggtgaacc cgcaattaat gcacatacag
    16501 ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc aaaccaggag aaaaagaaac
    16561 atccgacaat aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc
    16621 tgtttgcccg ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt
    16681 tattaaatgc gcgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca
    16741 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
    16801 ttccgataag ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta
    16861 tttaattacc agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg
    16921 ctgcagggtc gatgaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta
    16981 aagcatgacg aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca
    17041 ttaatttgtc ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac
    17101 tgagatcaac accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg
    17161 gcaggttata tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac
    17221 cttcaatggc gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac
    17281 cgatgcccat gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa
    17341 taatatttcg tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt
    17401 tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa gtaatttaat ggttgagtat
    17461 ctgtacttaa tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat
    17521 cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca
    17581 tactggatgc aatatcacca acgactggca cagcaatggg gcggttgctg tcaatttcct
    17641 gcggttcaat atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg
    17701 ccaataacca attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa
    17761 acgaacgcgc agctgccgca gaaagtggat gcgtatcttc aaggatccct ttcgccatag
    17821 acattggcag gaatggaatc tgagtacttt caataaattc acgaagctgt tcatcagctt
    17881 gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg
    17941 aaattgcgct agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt
    18001 taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca
    18061 aatcaagata aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg
    18121 caatgccaag atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg
    18181 cattcatttg gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc
    18241 tggagccgct aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgcggtca
    18301 aaccattgag gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa
    18361 aaccgcttgc cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa
    18421 tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa
    18481 tagtgtcaat attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt
    18541 gaagttgatc tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc
    18601 ccgttaatat cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc
    18661 cagctcctgc aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg
    18721 cgtaaaggca gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg
    18781 ttccacttca acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat
    18841 acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt
    18901 atcaatagtg acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg
    18961 tgcatgggct gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca
    19021 ggtgttttcc cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc
    19081 ccagccttta catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg
    19141 gggaactgca tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa
    19201 tttatcgaga cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg
    19261 cattgacatg gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag
    19321 taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag
    19381 cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt
    19441 ttcataggct tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa
    19501 aatcagacgt ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc
    19561 ccctggatga aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc
    19621 tttgccttcc gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt
    19681 gaagtaaagc gcatcgatat caggaatatc tcgcagctgg tgacgcgtta cgtcaccaac
    19741 gccgggacgt tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga
    19801 tgggccagat tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat
    19861 agcaatacct attagttaat taaatatgtg ttaatttagc gtttatttaa ggcgtaatat
    19921 cttctataaa taacccgcaa aatatcgtcg tagctgcact cgatattttt aaaatcctgt
    19981 atcaagtgct gaaaatcatt tcatcgccac ttccaattcc tttctgcatg gtaagtagtg
    20041 aggacgaaaa gaagaatatg ataaatattg attcgtggta atcagagaga gtcgcgacat
    20101 ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gggtatgatt
    20161 acgcttttta aagcgtacca taaaacataa cataataaat gatttgtgat gacacgggat
    20221 tattgggatt tactttgagg aagataaaat gcgtgtttat agaaccgcga gcgccaaata
    20281 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta
    20341 aatcctttac ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctgtcg
    20401 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
    20461 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat
    20521 tggttttcca ttacgccaac accggccagg cgaagttctt ccattgcgcc ctttttatcc
    20581 cctttcgcca ttttttcatt ggcaattttt atcgcggctt ctttttcggg agttgctaca
    20641 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgagattg
    20701 gtctttttac ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg
    20761 gaagcttcat tggtcagttc ttttgccttt tctggatcgc catgaaacag agccaggcgc
    20821 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc
    20881 gttttctggg tttgctgaac ttgctgagtt tgctgttgtg ccgctaccgg tgcattatca
    20941 gcagcccata cagttgaaga tgccagaatt gctgtgacca tcgtagccat aattaaacgt
    21001 ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat tgggtgagca
    21061 tgaaatgaga aatcagagag caacggtcat tttgaaaggg ttatcttaca gttgtagtta
    21121 ttatcgcaag aagtatttta gtcttacaat tgtagttatt atcgcaagaa gtattttagt
    21181 c
//
LOCUS       DAMZII010000084        20667 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZII010000084 DAMZII010000000
VERSION     DAMZII010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.084"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2483
                     /locus_tag="POA77_003021"
     CDS             <1..2483
                     /locus_tag="POA77_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6366412.1"
                     /translation="ASTTKRDKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNV
                     LFNYSVFDSSQTLLVMRPYQIAATERILWKINSSFKAKNWSKPESGGFIWHTTGSGKT
                     LTSFKAARLATELDFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSDNTAGLRRNLD
                     KDDNKIIVTTIQKLNNLMKAEADLPVYQQQVVFIFDECHRSQFGEAQKNLKKKFRRFY
                     QFGFTGTPIFPENALGAETTASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKS
                     LETETDEKKLSAAENQQAFLHPLRIQEITQYILNNFRQKTHRTFPGAKGFNAMLAVSS
                     VDAAKAYYTTFKMLQEEAEKKSGSYKRLRVATIFSFAANEEQSAIGDITDESFDTSAM
                     NSSAKEFLDSAIDDYNNHFKTNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTG
                     FDAPTLNTLFVDKNLRYHGLMQAFSRTNRIYDATKTFGNIVTFRDLERPTIDAITLFG
                     DKNTKNVVLEKSYEEYMQGFTDAATGEAKRGFMAVVSELEQRFPDPASIDSEKEKKAF
                     VKLFGEYLRAENILQNYDEFATLKALQKVDISDPEAVETFKAEHYVDDEKFAELQTIR
                     LPAERKVQDYRSAYNDIRDWQRREKAANDRDTSTTDWDDVVFEIDLLKSQEINLDYIL
                     GLIFDHNREKKGKEALTEEVRRLIRSSLGNRAKEGLIVDFIQQTNLDDMPDKASIIDA
                     FFTYAQREQQREAEALIKEENLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLN
                     PQYKTKKQTVFQKIGAFIDKFKGVGGNI"
     gene            2508..3674
                     /locus_tag="POA77_003022"
     CDS             2508..3674
                     /locus_tag="POA77_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366413.1"
                     /translation="MKKPTYNKSDRLPVRKNNPHTPEARERVIDRLRASAKADFVFIV
                     RWLTTGKSNGEPIPLARCDNLRNIISNLETLKAGIQPDWRKALTLMSREVHSRHKVTN
                     TAKPPSKIAVGVISNLNEISQKIASGKATHDMPARLNYMLQLTTSLEGGDELADSIRE
                     VTKSAEMALRGYSKSPQKQKILKHIQLPKVLRDDKAFRDECAHLAGISDIIIVANCLI
                     AMSARLGVSPVNLANRAAALKNVIKPEILPELLDLLQQDVDITALVPPPKVKDDNSRS
                     DGGKLVGLTGIVEGGASIGVEAQRIITAELKKHIPLTAVQRIALDTARTSLALECDAF
                     NVIEEIKRISANLNLRPLAVATYVAHKSPGIRSDIAGDVITLLAQDEVLSSNAT"
     gene            complement(4053..4589)
                     /gene="yghD"
                     /locus_tag="POA77_003023"
     CDS             complement(4053..4589)
                     /gene="yghD"
                     /locus_tag="POA77_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDI6366414.1"
                     /translation="MLRDKFIHYFQQWRERQLSHGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMVARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(4591..5769)
                     /gene="gspL"
                     /locus_tag="POA77_003024"
     CDS             complement(4591..5769)
                     /gene="gspL"
                     /locus_tag="POA77_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDI6366415.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5766..6743)
                     /gene="gspK"
                     /locus_tag="POA77_003025"
     CDS             complement(5766..6743)
                     /gene="gspK"
                     /locus_tag="POA77_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDI6366416.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6746..7345)
                     /gene="gspJ"
                     /locus_tag="POA77_003026"
     CDS             complement(6746..7345)
                     /gene="gspJ"
                     /locus_tag="POA77_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDI6366417.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(7342..7713)
                     /gene="gspI"
                     /locus_tag="POA77_003027"
     CDS             complement(7342..7713)
                     /gene="gspI"
                     /locus_tag="POA77_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDI6366418.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7710..8273)
                     /gene="gspH"
                     /locus_tag="POA77_003028"
     CDS             complement(7710..8273)
                     /gene="gspH"
                     /locus_tag="POA77_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDI6366419.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(8277..8732)
                     /gene="gspG"
                     /locus_tag="POA77_003029"
     CDS             complement(8277..8732)
                     /gene="gspG"
                     /locus_tag="POA77_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDI6366420.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8749..9972)
                     /gene="gspF"
                     /locus_tag="POA77_003030"
     CDS             complement(8749..9972)
                     /gene="gspF"
                     /locus_tag="POA77_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDI6366421.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9972..11465)
                     /gene="gspE"
                     /locus_tag="POA77_003031"
     CDS             complement(9972..11465)
                     /gene="gspE"
                     /locus_tag="POA77_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDI6366422.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(11465..13525)
                     /gene="gspD"
                     /locus_tag="POA77_003032"
     CDS             complement(11465..13525)
                     /gene="gspD"
                     /locus_tag="POA77_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDI6366423.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(13555..14514)
                     /gene="gspC"
                     /locus_tag="POA77_003033"
     CDS             complement(13555..14514)
                     /gene="gspC"
                     /locus_tag="POA77_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDI6366424.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLHTDKEHLRKIARGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(14532..14942)
                     /gene="gspS2"
                     /locus_tag="POA77_003034"
     CDS             complement(14532..14942)
                     /gene="gspS2"
                     /locus_tag="POA77_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDI6366425.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCTDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(15008..15817)
                     /gene="pppA"
                     /locus_tag="POA77_003035"
     CDS             complement(15008..15817)
                     /gene="pppA"
                     /locus_tag="POA77_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDI6366426.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(16144..>20667)
                     /gene="sslE"
                     /locus_tag="POA77_003036"
     CDS             complement(16144..>20667)
                     /gene="sslE"
                     /locus_tag="POA77_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDI6366427.1"
                     /translation="ILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEP
                     TPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGF
                     TFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNAL
                     SLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATD
                     KAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQGDGVV
                     GVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGA
                     NIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNL
                     PNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTN
                     YKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWD
                     KNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNG
                     YSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFK
                     RHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAI
                     PLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSM
                     ALNKSVVNNDPQGYPNRVRQRRATGIWVYERYPVVEGELPYTIDSQTGKVTWKYQIDN
                     KPDKKPKLEVASWQEEVEGKQVTRYAFIDEAEYTTKESLDAAKKKILDTFKGLKECTD
                     PTYHYEVNCLEYRPGTNVPVTGGMFVPQYTQLNLSADTAKAMVQAADLGTNIQRLYQH
                     ELYFRTNGRKGERLSSVDLERLYQNMSVWLWNKIEYRYENDKDDELGFKTFTEFLNCY
                     ANDAYAGGTKCSADLKKSLVDNKMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRSW
                     WDLNIKVDVEKYPGAVSAEGEKVTESISLYSNPTKWFAGNMQSTGLWAPAQKEVTIES
                     KANVSVTVTVALADDLTGREKHEVALNRPPKVTKTYDLDAHGTVKFKVPYGGLIYIKG
                     NSPTNESANFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDSFVYTTPKKNLEASNY
                     KGGLEQFAKDLDTFASSMNDFYGRNGESGNHRMFTYEALTGHKHRFTNDVQISIGDAH
                     SGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQ
                     DRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKQWY
                     PEGNLPKFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLC
                     ASWVAQTDLSAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTLASLGLPKPQQGPE
                     TINQVTEHKMSAE"
BASE COUNT         4868 a         5698 c         5133 g         4968 t
ORIGIN      
        1 ttgccagcac cactaaacgg gataaaaaca gctttgattt caccatgaac tgggcgaaat
       61 cggataacac tctgattaag gacctgaagg actttaccgc caccttcttc cagaaacata
      121 ctctgctgaa tgttctgttt aattacagcg tgtttgacag cagccagacg ctgctcgtca
      181 tgcgccccta tcagattgcc gccactgagc gaattctctg gaaaattaac agctcattta
      241 aagcgaagaa ctggtcaaaa ccagaaagcg gcgggtttat ctggcatacc accggctcag
      301 gtaaaaccct gaccagcttt aaggccgcac gactggcaac cgagctggac tttattgata
      361 aagtcttctt cgtggtcgac aggaaagacc tcgattacca gaccatgaag gagtatcagc
      421 gcttctctcc ggacagtgtc aatggttcgg ataatacagc aggcctcaga agaaacctgg
      481 ataaggacga taacaaaatt atcgtcacaa ccattcagaa actcaataac ctgatgaaag
      541 ccgaagctga cctgcccgtc tatcagcagc aggtggtgtt tatctttgat gagtgtcacc
      601 gcagccagtt tggtgaggcc cagaaaaatc tgaaaaagaa attcagacgc ttttatcagt
      661 ttggttttac cggtacgcct attttcccgg aaaatgcact gggggcagaa accaccgcca
      721 gcgtcttcgg gcgtgaatta cattcttacg taattactga tgcgattcgt gatgaaaaag
      781 tgctgaagtt taaggtggac tataacgatg tccgcccaca gtttaagtct cttgaaacgg
      841 aaacagacga gaaaaaactc agcgcggctg aaaaccaaca ggcatttttg caccccctgc
      901 gtatccagga aataacgcaa tacattctca ataacttccg gcagaaaacg caccgaacct
      961 tcccgggagc taagggcttt aacgccatgt tagcagttag cagcgtggac gcggcaaaag
     1021 cttattacac gacgtttaaa atgctacagg aagaagcgga gaagaaatct ggcagctata
     1081 aacgccttcg ggttgcaacc atcttctcct ttgctgcaaa tgaagagcaa agcgccattg
     1141 gtgatattac tgacgaaagc tttgatacca gcgcaatgaa cagcagcgca aaggaattcc
     1201 tggattcagc cattgatgac tataacaacc actttaaaac caatttcagt accgacagta
     1261 acggtttcca gaactactac cgcgatttag ctcaacgagt gaaaaatcag gatattgacc
     1321 ttctcatcgt ggtgggaatg ttcttgactg gctttgatgc tccgactctc aatactctgt
     1381 tcgttgacaa aaacctgcgt tatcacggtc tgatgcaggc attctcacgc accaaccgta
     1441 tctatgatgc aaccaaaacc ttcggcaata tcgtgacttt cagggacctg gagcgtccga
     1501 ccattgatgc catcacactt ttcggcgaca aaaacaccaa gaatgtggtg ctggaaaaaa
     1561 gctacgaaga gtacatgcag ggctttaccg atgcggcaac cggtgaggct aaacgaggtt
     1621 ttatggctgt tgtttcggag ctggagcagc gcttccctga ccctgccagt atagacagtg
     1681 aaaaagagaa aaaagccttc gtcaaactgt ttggtgaata tctgcgggca gaaaacatcc
     1741 tgcaaaacta cgatgaattt gccacgctga aagccctgca gaaggttgat attagtgacc
     1801 cggaagcggt agaaaccttt aaagcagagc actatgtgga tgatgaaaaa tttgctgaat
     1861 tacaaaccat tcgtcttccg gcagaacgta aagttcagga ctatcgctca gcatacaatg
     1921 atatccggga ctggcagcga cgcgaaaaag cagcaaacga cagggataca tcaaccacag
     1981 actgggatga cgtggtgttt gaaattgacc tgcttaagtc gcaagagatt aacctggact
     2041 acatccttgg tctgattttc gaccacaaca gagagaaaaa aggtaaagaa gccctgactg
     2101 aagaggtcag acgcttaatc cgctcaagtc tgggcaaccg tgccaaagaa ggtctgattg
     2161 ttgactttat tcagcaaact aaccttgatg acatgcccga caaagctagt atcattgatg
     2221 cattctttac ctatgctcag cgtgaacagc agagggaagc ggaagcgttg attaaggaag
     2281 aaaacctcaa tgaagaagct gcaaggcgct acatccgcac atcactcaaa cgggagtatg
     2341 ctaccgaaaa tggcactgag ctaaatgaga cgctaccaaa gctcagtccg ctgaatcctc
     2401 agtacaaaac gaaaaagcag accgtattcc aaaaaattgg tgcctttata gataaattca
     2461 aaggtgtagg cggaaacatt tagcatgggc taatttgaga tagtgatatg aaaaaaccaa
     2521 cttacaataa aagtgatcga ctgccagtac gcaaaaataa tccccatacg cctgaagcaa
     2581 gggagcgggt gattgatagg ctgcgagcct ctgctaaagc tgatttcgtt tttattgttc
     2641 gttggttaac tactggaaaa tctaacggtg aaccaatacc actggcaaga tgcgacaatc
     2701 ttcgtaacat catcagcaat cttgagacgc taaaagccgg tatccaaccc gactggcgta
     2761 aagcgcttac attgatgtct cgagaagtac attcaaggca caaggttact aatacagcaa
     2821 aaccgccatc aaaaatcgca gttggggtaa tcagcaattt gaatgaaatt agccagaaaa
     2881 ttgcatcagg gaaagccacg catgacatgc ctgccagact caattacatg ctacagctta
     2941 ctacgagtct ggaaggcggc gacgaactgg ccgatagtat cagagaagtt acaaaaagtg
     3001 ccgaaatggc tttacgtggt tattccaaat caccgcagaa acagaaaata ttaaaacaca
     3061 tccagctccc aaaagtacta cgtgacgata aagctttccg ggatgaatgt gcacatctgg
     3121 caggtatcag tgatattatt atcgtagcaa attgtctcat tgctatgtca gcacggctag
     3181 gggtttctcc tgtcaacctt gcgaacagag ccgcagcctt aaaaaatgtt atcaagccgg
     3241 agatactccc cgaacttctg gatttgcttc agcaggatgt tgatataact gccttagttc
     3301 cccctccaaa ggtcaaagat gataatagtc gcagcgatgg cggaaaactt gtaggactga
     3361 cgggaatagt tgaaggaggc gcatctattg gcgttgaagc tcagcggatt atcacagcag
     3421 aactgaagaa acacatcccc ctcactgctg ttcaacgaat tgctctcgat actgcgcgta
     3481 cgagccttgc actggaatgt gacgcattca acgttatcga agaaatcaaa cgcatctcag
     3541 ctaacttaaa cttgaggcct ctggctgttg ctacctatgt ggctcataaa tcaccgggaa
     3601 tcaggtcaga tattgctggg gatgtaatta ccttgcttgc tcaggatgag gttctttcat
     3661 ctaatgcaac ataggtatca gacctccgaa gctacacttt aataagccat ccgtactcaa
     3721 tggtagttat ctggtagctt cgaaacgtct catttgaacc tcatttaggg cttatagccc
     3781 ccaactgcaa catttttctt aaggtaaacc agtaaaaggc tgtaagttct aatagatcgt
     3841 ttggtctttt tttaagggat tgaaaatccc cgtgtccttg gttcgattcc gagtccgggc
     3901 accactaatt cttaagaacc cgcccacaag gcgggttttt gcttttggat ctgacaataa
     3961 ccttcacgaa aaaagtttgc ttataaagtc tgggggaatt actctcgcca cgttaacgag
     4021 agtaatttta ttgatactaa tctcctgata ctttaccccc gtccaaactc cagccgctgc
     4081 acattcacca tcccagtctt ctcagcagca ctggcatcaa tttgtgtcac ccgcagcgca
     4141 tacttttcat ccagcgcgtt taaccagttc agcaggtcat taaacaccac cggatctatc
     4201 cagacctgaa tattctcccc acgctcggct atccgcttga tgactaccga gtgcgcggaa
     4261 gcgctgtcac tgataacccg cgatacctgc gcaggcgttg ttgtggcgga ttttcgcgcc
     4321 accataatat ccggcgcggc gctcctcagt ctggtgttca tcgccaccag ttgctgcaac
     4381 attgtctcct gttgttctat ccgttcgctc aacggctgcc agatgaggac gtaatatccg
     4441 acgctaaaca ggaacaccac cgctgccagt aacatgccct tttcacgcgg agaacgcccc
     4501 gccaggtgtt gtgccagcca gtgttcgcca tggcttaact ggcgttcacg ccattgctga
     4561 aaatagtgaa taaatttatc gcgtaacatg ttatttcctc cgcaacgtta cgccgccgga
     4621 aaccgcatca ccctctttct gtaatgcgtc ctgttgcaca acataatcgg ttgccagtgc
     4681 actacgcagt ttgtcgaaac tggcaaagtt agcagcccgc aactggaggt gaagcgtctg
     4741 gcgtttttga tcaaaggtga aaccacgcat ttcgatgtcg gaaagtgacg ctgatttcag
     4801 ggtgctggct atcgctgaca actcggcgag cagccgggta tcgtcggcct gtgggcgata
     4861 ttttttcagc gccatcgtca cctgagagcg taaattcaca atccgcttct gctccgggaa
     4921 caacgttaag aactgttcct ccgcctgggt gcggctttgc gccacctgtt cgctgacgct
     4981 ccataacgtc acgccccgct ccactgccag cgcaaccaga atcagcaata tcggcagaat
     5041 catcacccgc cagcgcgccc actgttttcg gtagctgacg cgaggctgcc acggccctgt
     5101 cagcaggttc ccttccggtt cgccataagt ggtaatggcg ggcagagctg taacggtcag
     5161 gcgttcagcg tctgcgccag cccatgctga tagcttttcc ggtgcaatgc cgactacggt
     5221 tagcgaaagc ggtaaattct gctcattgag ctgggcgcgg aacatgaccg gagccagcgc
     5281 ccgcccggcg ctccatcccc ggcattcatc gatgcggcag ataacccgtt gtgcatcgcc
     5341 agccataaac ccacaaggaa tggacatcca gtccggcgcg acggtagcgc gggtgatgcc
     5401 gttttcctgc aaccactgcg caatgttgcg catatgctgt tggtgaatca cggctacggt
     5461 tgccagttgc tgattgattt tcaacggggc gaaatgcagt tcatcgatat cctggttcag
     5521 ttcttcttcc agtaaggcgg gcagaatagt cggtatctgc ttgcgggcga caacaggcag
     5581 ttcaacctgc cagacgctga tccattcgcc gggaatgtag agtcgaatcg catcagtttg
     5641 cagccattgc tggagctgtt cgttagcaac gtcagaccag ataccgtgct ccacatcggg
     5701 tgtacggcgc tgccaatgga tgggatcagc ggcgcaaagc gggaaaaaaa tctcaaggat
     5761 ggaactcact cactttctcc tgtctgatgc cagagaaccg aaaagtgttg tgggcccatg
     5821 cggacaatta acgagttcat cgtcagttca atctcattca cggtgatatc tgaacgcagc
     5881 cagaagtaat tgctgtccac gctcaggacg gtttttagct gttttttagt acgctcatcg
     5941 acgtcagcaa gtagcggctg tgcaagaaac tgatcgacat cttcccagcc cttcgccgga
     6001 cgttgttgta ataacgcccg cgcctgaaca gggcttaacc acgggtcaaa cagcgcctca
     6061 agaatcacac tttgcgtgac gtctaaagta ttaatgttga tttgctggcg ggtcatcggc
     6121 agcgcacaga ccagcggttt cagtttttga taaagcccgg cgtccattcc ctgcaccacg
     6181 cgcatctcgc tgatatcggc cagcggttga ttggcggcgt agaaaggcac cgagcgggcg
     6241 agatattcgc tgtcttcacg gcccagacgc gtctgcacgc tgcggtcctc gtcaataaac
     6301 tcccacaggc tttcggctat cagttcggcc cgataagcag gcacatccag gcgcgtgatc
     6361 agggcaatca gttgttgtac cgcgagcgga cgcgacgccg ttgtgggctg agcgagggca
     6421 ttcaggttaa agcaggcctg tgcgtcacgc agagtgacgg cgatttgccc ctgcggcagt
     6481 gggaaaaaac gagggccgga agcccacggc tgttccagat gcacgcgctt ttcatttttc
     6541 aggctcagac tgagcgcgct cagcgccagg ctttctgcac tggcgctgta ccacagcgcc
     6601 tgctggtact cctgctggct gcgcgttctt cccagttgct gctgcatccg cccggaaagc
     6661 gtgatggtta ccagcatcat aactgccagc aataccagca ccacgaccag tgccattccg
     6721 cgttttggtg gtgaggtgat catgattatt gcggcccgcg taacaaccag atgcgttcaa
     6781 tctcgcccca ttgcggcgaa tgcagggtca tgcgtaccgc cacagggatc gcctgtactg
     6841 acgaccagct ctcctgccag cgcgtgccgt cgtagaactg caaacggagc gaatcggccg
     6901 gaatcaactt ttgcattgtc ggcttcacgc tgcctgcggc atcggtcagc ggccaggcta
     6961 accgttcgag ataaccgtca tgaatgcggt aaccgacggt gagcagatta ctgcgcggca
     7021 gacgcatcaa cgggttaacc acaccgccgc gcacaaaacg cattccttca ctctcagacg
     7081 ccagcacgcc agcgccagcc agtaacgctg gttcacgctg gccctgatcg cctcttaccg
     7141 gacgcggcat catttgggtc agatcgtggg tcagaaaact catcgtttgc tgcatgaggt
     7201 ttagtttttg atcgtgtccg gcgacggcgc tattcacgcg cgtgacgccg ttagtcacct
     7261 gctgcgccat cagtgccagc gaggcaaaaa tggcaattgc caccaacatt tccagtaacg
     7321 tgaaaccagc gcgagtcctt ctcactgttg gcctcccacg gcgctaaacc aggcgcgtcg
     7381 tgactgaatc accgacgaaa agtcttcgtg aaggctgact tcaatatcca ccgcctggag
     7441 cagcgcgttg cctgtactca gcggtgtggt tcgccagtac cagcggcttc ctgccatatc
     7501 gctctcgccc tgctgcctga ccgcccgctg ctcgcgggtc atcaggtaga gcagcgcggt
     7561 ctggttttca gccacccagc ccgctaccgt tttttcctca agaatttgct gattactcag
     7621 cgcaccgctg gcaatctgta acaccgccgt ggcagccagc gcaaaaatcg ccagcgcgag
     7681 catcacttcc agcaaggtaa atccgcgctt catggcatcc tctcatcaca ttgattgagg
     7741 gataacgcgc catcgtgtgc cagctttacc gcccaacatg cgttttgcgc cgccgagtag
     7801 aagcgcagcg taaacggcgt ggcgggttca aaaggcgaaa aacggatctg cggcgtcttc
     7861 tttttcgcct ctttttgcag ctcaagttca atatcgtgca gcgtcagtcg acgacgttgc
     7921 agctccagcg catattcctt ctgccagata tcacttccgg gttgtaacag catctgcacc
     7981 tgtttcggca ccgtaacctg tgccgataac cgggtcgagg aaacgggcga ccactgcccc
     8041 tgacgacgct gcataaactg gtagccaggc gggtcgatta gcacaccgag tgttttccct
     8101 tcgatcactg ccctgtcctt aaactgcgca aagcgagtca gaaaatcctg cgccgctttt
     8161 ttcgcgggcg attcagaatc ggtcgcaaaa gtctgtacca cgcccgcact ggcaaggccg
     8221 ataagaaaaa tcaccagcat gatttccaga agtgtgaatc cgcgttcagg cactgcttac
     8281 tgaaactcct gcaaattcca gttaccgata tctgcgccag cgccttcccc gttttcctga
     8341 ccatccgccc ccagggtata aacatcaaac agcccttttt cacccggact gagatactga
     8401 taatcattgc cccacggatc ctttggcagt cgtttaatgt atccaccagt acggtagttg
     8461 cgggagtccg ccatattggc tggctgctgg atcagcgcct caagcccctg ttctgtggtc
     8521 ggataacgcc cgttatccag tcggtacata tccagtgcgt tttccagcgc cacgatatcg
     8581 ctgatggctt tttgccgatc ggctttctct ttgttgccca acaggttagg caccaccaga
     8641 cttgccagga cgccaagaat aacaatcacc accatcactt ccagcagggt aaaacctgcc
     8701 cgtggttttt gtgtgcggga taacgaattc atgtgataac tccgtaaatt acattccaac
     8761 catattattc agttgcagca tcggctcgag gatggcgatg acaataaaca gcaccacgcc
     8821 cgccatcatc accaccagcg ccggttcaaa cagccctaac gccagcccca cctgggtatc
     8881 aaattcccgt tcctggttga ctgcggcctg ctcaagcatg gtttccagtt cgccgctctg
     8941 ttcgccggag gcgatcatgt acagcatcat cggcgggaac aggcgcaact ccgccagcgc
     9001 ggcgcgcaga ctgcttcctt cgcggacgcg atctgccgcc agcagtagtt gttgttcgac
     9061 atagcgattt gccgacacgg cggcagcagt ctgaatacct tccagtagcg gaacactgct
     9121 ggcggtgagg atgcttaagg tgcgggaaaa acgcgccgta ttcagcccgc gcgcaacacg
     9181 ccccgtcaca ggcaggcgca gcacggtttg atcccagcgc aggcgcatag caggattttt
     9241 aagtagccgt tgccccagca ccagaagccc cagcaaaccc gccagccagt aaacgccgct
     9301 ggcctgtaac gcgtcgctca tggcaataag cgtgcgggtt gaggcgggta gcgcgtgtcc
     9361 gagatgatca aactgttcga tgattttcgg caccactgcc gtcagcaaaa tagtgactac
     9421 gcccgttgcc accaccagca gaaccagcgg atagagcatg gcctgcaata gacgtgattt
     9481 caggcgctgt cgctgttcgg tgtaatctgc caggcgatta agcaccacgt cgagatgccc
     9541 ggatttttct ccggcagcaa ccatcgaaca aaacagagaa tcaaagacgc ggggatgttc
     9601 gcgcaggctg tccgacaggg tgtaaccttc ctgaatccgg ctgcgcagcg ccattccgag
     9661 gctttttaca tgcagttttt cactttgctc actgaccgcc tgtaagcagg tttccagcgg
     9721 cattgctgcc tgtaccagcg ttgccagttg gcgcgtgaac agcgcaagat ctgccgccgc
     9781 cacgcgacga tgtgcgtgcc gccgacgctg caacatcccc cctgacgaag tattcatccg
     9841 ggcttcaata tgcacgggga taagctcttt accgcgcaac aactggcggg catgacgcgc
     9901 ggaatccgcc tcaatcatac ctttggtttt gcgaccatta cgctccagcg cctgatagta
     9961 aaacagtgcc attacgcctc catggttacc cgcagaactt catcgagaga ggtttctccg
    10021 gcgagcactt tctcaatgcc gttgctgcgg atacccgcag agtgttgtcg gacataacgt
    10081 tccagctcca gctccccggc ctgacggtgg atcaaatcac gcaatgtggc atccaccacg
    10141 atcagctcat ggatggcagt ccgtccgcga aaacctttgt gattacaggc gggacagccc
    10201 tgtggatggt acagagtgac ggtacgggcg tcggtaattc ccagcaggcg tttttcttcg
    10261 tcagtggctg gtgcgggctg gcggcagtcg ggacacagcg tgcgaaccag tcgctgcgcc
    10321 atcacgcccg tcagactgga agagagcagg aaaggctcca cgcccatatc ctgcaaacgt
    10381 gtgatcgccc ccaccgctgt gttggtatgc agcgtggaaa ggaccaggtg tccggtaagt
    10441 gaagcctgga cggcgatttc tgcggtttcg gtatcgcgga tttcaccgac catcaccaca
    10501 tccgggtcct gacgcaaaat cgcgcgcagc ccacgggcaa aggtcatgcc gacgcgggtg
    10561 ttaacctgcg tctgaccgat cccttcaatc atgtattcga tgggatcttc aaccgtgaga
    10621 atgttgcgcg aatggttgtt cagctcctgc aatccggcgt acagcgtggt gcttttgccg
    10681 gaacccgtcg gccccgtcac cagaaagatg ccgtgcggtt tgtgtaacag ctggcgcaac
    10741 tgcgcggtca gttgctggct aagccccaga cgctccagcg tcaggcgggc ctggtttttg
    10801 tccagcagtc gcagcaccac gcgctcgccc caggcagaag gcatggtgga gacacgcacg
    10861 tcaatcgccc ggccgcccag caacagcgca atacgtccat cctgcggcac gcgcttttcg
    10921 gcaatgtcca gccgcgccat caccttgata cgcgacacca gcagcgaggc cagtttgcgc
    10981 cccggacgca gcatttcatg taatgtgccg tcaacacgaa aacggatcac cagactcttt
    11041 tcaaacgtct caatgtggat atccgaagcg ccttctttga ttgcctctgc cagcatggcg
    11101 ttgatcagtt tgatgatcgg cgcatcgtcg tcgctttcca gcagatcttc cgtttccggc
    11161 agttcttccg cgagggtaaa aaagtcctcg gcagaaccga gatcttccat cagttggcga
    11221 gcctcggaag agtcccgctg ccagaccgca ttcagccgct gttcaaattc ggcttcgtcg
    11281 atttgccgca gcgtaaaggg cgcgttcagc ccccgctgca gctcctgcaa aacagagagc
    11341 gatagcggat gaacgtggag gatctccagc gacgcttcgc accatgccac caggctaaac
    11401 cgacggctga aactgtaggg cagacgcacg gtgttggcgg tggtttcctg tgctacaggc
    11461 accattaacg cgttctcccg gcattgagga acgcgcgaac ttccggcggt aaggcctggt
    11521 tttgcgctgg cagtaccggc tgcgcggtgt gcggcatcag gcttaaacct tgctcatcac
    11581 gatagatctg ttcggcgcgc atatagttat atttgcgctg cgacacgccg tctgccgcca
    11641 taccgtcacg cagaatggtc ggacggataa acaccatcag gttacgtttt tcttttttat
    11701 ccgccgtcga tttaaacagg ttaccaatca atgggatatc gccaagcagc ggcactttcg
    11761 ccacgctttc tcccgcctgg tcgtccatca gaccgccaag cacaatcagc tcaccatcgt
    11821 tagccagcac ggtggttttc agtttgcgct caccaaacac cacgtcgagg ctggtctgtc
    11881 cttccacctt cgagacttcc tgctcaatca ccatctgtac cgcgttacct tcgttaatct
    11941 gcggcgtgac tttcagcatg atgccgactt ttttcctttc tactgtattg aaaggattgc
    12001 tgttattgga gccaacggta gagccggtta ataccggaac gtcctggccc accatgaaga
    12061 aggcttcctg gttgtccagc gtggtgatgc tcggcgtgga gagcacgtta gagctggagt
    12121 cgtttttaac cgcctgcacc agcgccatcc agtcgccttt caccacacca accgccgtac
    12181 cgctaaagcc ggaaagaagc tgggcgagtg tggagagatc gccgttggta tccggattaa
    12241 tggtggtagc gccgttttca ctgatcaccg tcgagccttt ctgcggtttc gcctgagaaa
    12301 tggctgcccc cagcgtaccg atagggatct gcgtaccatt agcaaactgc attaatccgg
    12361 catctttcga cgcccactgc acgccgaagt tgatattgct accttcggca acttccacga
    12421 tcaacgcctc gacgtgcacc tgagcacggc gaatatccag ttgttcaatc acgctttgca
    12481 gcgactgcat gatgtcctgc ggtgcggtta caatcagggc attactgtgt ttgctggcgg
    12541 cgatggatac aatctcacgc ccgctaccga ccgtgccttc cgcctcttct ttagccgccg
    12601 tgagcgtgcc gctgacctgc ttcagcacat cgaccagatc ttcggctttg ctgtatttga
    12661 gatagaacac ctggctgttg ccgctgcgtt ccatttctga gtccagccga cggatcaggc
    12721 ggcgcatttt gtcccgcgtg gccgggtcac cactgacaat cacactgttg gtgcgttcgt
    12781 cggcgacaat ttgagatttc agcgtcgcag gctggttctc gccgctgttt ttcgtcaggc
    12841 tttccagcac gcgggcgatt tccgaagccg aggcgttatc cagcgggatc acctcttcgg
    12901 tgcgattacc cgcgtgatcc acgcgctgga tcacttctgt caggcgctcc acaacggagg
    12961 cgcgtccggt gagcataatc acgttggagg gatcgtaatt aacaacattg cctgagcctg
    13021 cgctgtcgat catctggcgc agaatcggtg ccagctcgcg taccgacaca ttacgtaccg
    13081 gcactacttt ggtgaccatt tcatcgcccg cgtagttgtc gctgccttca ccgaccagcg
    13141 gcagcggctc gactttcgca gcgctggatt tcaccacctt cagcacgtcg ttttccatcg
    13201 gtacgacggc atacccctgt gcttccagca ggttaaggaa tagctggtaa tactggcgtt
    13261 cattgagcgg ggtcatggtg cgaatactca ctttcccctg tacgcccggc cccataatga
    13321 tggttttatt aaggttagcg ccgacggttt cgatgaacga tttcaggtcg gtatctttaa
    13381 aattagcggt gaaagtggct tcttccgccc agaccggtga actacataat gccgctgcca
    13441 gcaccagcgg cagtaaacgc ttttttgttt tgaggccagt tgtcttctta cgccagaccg
    13501 acaacgtaat atcacgccaa aacacgatga atgattctcc tggattaaat gcggttagcg
    13561 cagcgcgatg gaaatgtcgt ggcgcgcacc cttgcgtaaa accgtaagtt gaatggaatc
    13621 cattgaaggt aactgccgca tcagagcaat cattgctcgt ggatcagtga aatcctgctg
    13681 atttagcgca atggcgatat cgccttcctt gaaaccgctg gcatcgaaca gagaacgatc
    13741 tgcccccggc ttcactgcat aaccgacaat cccctcctta cgcacaggcg taagctggat
    13801 atagttaaaa attttctgcg gatctttcgc cagtgcctga cgcacggcag tcgggatctc
    13861 aactggcgca ctggcagcag gctcagcaac agcttgcttt gcttcgtcac tgacagcttt
    13921 tttgttggtc acggcaacgg tggaacgctc ctcttcagcc agactcaggc gctctatttt
    13981 tccctgatag cgcagcatca catggtcgcg attgatttcc tcaatcactg cgttgtgcga
    14041 gccaagcgtt tcaccctgca aatagacctg ctgtttaccg ccttcttcaa taaccgcgcc
    14101 gggtctggca ccaaaggcga tcccgcgcag caccacatta agacgcgttt ctgccacagg
    14161 cacaggttcg ggttgtttta ccggcgtggc gacgggctga tatttgccaa accagttttg
    14221 ctggctgatt aattgcacgt catttttatc gaacgttttt gcatccgcac ggggcggttt
    14281 attcgccgat ggggaaacag ccgtatattc cgcagaaaag gagaaatagc gccagagtga
    14341 atacgccatt tttgcagaaa taataagcag catcagccag aacatcccgc gtgcaatttt
    14401 gcgcagatgc tctttgtctg tatgaagaga ttgtctctgg ttaagagtgt gacgaatttt
    14461 tgtcagccat tgaatgagat aaattcgtgc gtcacgaaaa acaacccgcg ccaatttata
    14521 tctacccgac gttatgcttt gactattcca caggtggtac gatccagttt ccgctgatac
    14581 tggttacctg tacgtgtatc attaatcgtt atgctgtaat taattccctc agtgagcatt
    14641 aatttcactg ttgggtcagt gcacatttgt cgttgatagc tggttaaaaa ggcgtcaggc
    14701 gtctgcgtgg tctgagtccc cgcttcgctg ataatggtca tttttaccgt cgtgccgcta
    14761 ctttgtgcca gcactaaggt atatcccgca gatttaatcg gcaggttttg gctgatattt
    14821 tgcgcctgtt ttttcgccag taaactggca ttttcattat ggctggcaca gccacttaat
    14881 aatcctgtaa cgctcaacaa tagcgatatt aataccctcc ctggcatttg ttttatcgac
    14941 atggtaaata atctctaagg ttattaataa gagttaaaat gtcactttga taatgacgtt
    15001 gttatcatta aaacaatgcc tgtagataaa ttgttgctat accgcccaga cttaaacacg
    15061 gtccaaaagg cagtgtggtt gatcctcttt ttgtaataac ggcatatatc aggccgcagc
    15121 atgaggcgat taaagcaaca ttgggtagcg acaacggccc cacccaacta cctaacgcag
    15181 cgaacaataa tacatcgccc atgcctaatg cttctttacg cagaactatt ccggctatcc
    15241 agcgcagaga gtaaaaagtg ataaacccca ccaggacgcc ggtgactgca tcttgtagcg
    15301 tgagcgggct ctgctgcgcc catgccgcaa ttagtcccgt ccacaatacg ccctgagtaa
    15361 aaacatcggg cagccattgg tgatcgaggt cgatgatgct cgcggcaatc agccaggcgg
    15421 ataatatcat caccgccagc gcccatccac tttccggcca gaccagactc gccagcaaaa
    15481 aagcgagtgc cgtcaataac tccaccagcg gataacgctt gctgattttc gcctgacagt
    15541 cgcggcagcg ccctttgagc atcaaccagg agagcagcgg aatattgtca cgaacgcgga
    15601 tagtctgctg acaatgtggg cagtgcgaac gcggtagcgc aaggcttatt tttgactgcg
    15661 cactcggcat ttctccatga aactccgcca tttgttggcg cagcatgatg gggtaacgcc
    15721 aaatcaccac attcaaaaaa ctaccgatga ttaatcctcc gacggtagcc agtatgggca
    15781 tcgccacggg gtattgctga aaaacatcaa aaagcatggt taaaggttgt ttgttgtaac
    15841 ttgccggatg cggcgtaaaa cgccttatcc ggcctacagg tatctgccaa cgcaagtaat
    15901 cgcggctttc cctgaagacc cgataagatg gcgcatcggg catatgtgtt aacccgatgc
    15961 gccttatttc atgctggatg cggcgcgagc gccttatccg gcctacaggt gtctgccaac
    16021 gcaaataaac gcagctttcc ctctctgtag gcccgataag cttgcgcatc gggcatatgt
    16081 gttgacccgg tgcgccttat ttcatgccgg atgcggcgcg agcgccttat ccggcataca
    16141 ggcttactcg gctgacatct tatgctcggt aacctgatta atggtttccg gcccttgctg
    16201 cggtttcggc agaccaagcg acgccagcgt gttgtatgcc gactggctca caccgccctc
    16261 gaagctcatc tccgtcgctc ccggcaactg gtaagcattc gcgcccggat tccatttctt
    16321 aaagaatgcg gaaaggtccg tctgagcgac ccaggaggca cacagcatca gcgtgtccgc
    16381 agcgttaccg ttggattcag cacagtaatt cttgccacca aaggtcttgt caccaacgtc
    16441 atcgccgcgt gctttacgat gcatcaactg gaacaggttc cagcctttca tcccttcacg
    16501 ctcgctgtag aacttaggca ggttaccttc tggataccac tgtttgatat caaagttttt
    16561 ctctgcccat tccttcagct gcgcgtacat cagcagacgg tcacccgcac cgccacgtgc
    16621 ccatgcctgg ttgttgctct cctccagata ttccggtgcg acggtaatat cgtcagcaac
    16681 acggttcatc ttgccgagat agcgatcctg catgtacagc gccagcacgt tgttcgccac
    16741 ttcggtcgcg cctggaacag tcagcggcgt ttctgcggcg ttgtgcccta cttcgtgcca
    16801 gatcaaccag tcgttcagcg gcgtcgtcgg cagcgtggtg ctgttcgtcg agaagctgct
    16861 gttcattacc ggataacccg agtgcgcatc accgatggag atctgcacat cgttggtgaa
    16921 acgatgtttg tgacccgtca atgcttcata ggtaaacatc cggtggttac cgctttcgcc
    16981 attacgacca tagaagtcat tcatcgagct ggcaaaggta tccagatctt tagcgaattg
    17041 ttccagaccg cccttgtaat tgctggcctc aaggttcttc ttcggtgtgg tgtagacgaa
    17101 agagtctgac tccagctcac ccagcggagc cggtgagttc agagcgtttt tccattcgcc
    17161 gtctttatag aacggcgctt ttaccacgcc agtaaaggtg aagttggctg actcattcgt
    17221 tgggctgttg cccttgatat aaatcagacc accgtaaggc accttgaatt tcaccgtgcc
    17281 atgagcatcc agatcatagg ttttcgtcac ttttggcgga cggttcagcg caacttcatg
    17341 cttctcgcgt ccggtcagat cgtcggccag cgccacggtg acagtcacag aaacgttcgc
    17401 cttagactca atggtgacct ctttctgagc cggagcccac aggccagtag actgcatgtt
    17461 acccgcaaac catttggtcg ggttcgagta caggctgatg ctttcagtaa ccttctcacc
    17521 ttcagccgat accgctcccg gatacttctc gacatcaact ttgatgttca gatcccacca
    17581 ggaacgcccc agcatcaggc gcgtcagcgg tttttccata tagttgagcg gatagctcgg
    17641 gttcatcatg cccgctttgc cactactttc accgtagatc atcttgttat cgaccagcga
    17701 ttttttgaga tctgcagaac acttggtgcc gcctgcataa gcatcattgg cgtagcagtt
    17761 caggaactca gtgaacgttt taaagcccag ctcgtcatcc ttgtcgtttt catagcgata
    17821 ttcaatttta ttccacagcc agaccgacat gttctggtac agacgttcca gatcgacgct
    17881 gctcagacgc tcacctttgc gaccattggt acggaagtaa agctcgtgct gatacaaacg
    17941 ctgaatgttg gtgcctaaat ccgccgcctg caccattgct ttcgcagtgt cggcgctgag
    18001 attcagttgc gtatactgcg gaacaaacat gccaccggtt accggaacgt tcgtgccagg
    18061 acggtattcc agacagttga cctcgtagtg atatgtcggg tccgtacact cctttaatcc
    18121 cttgaaagta tccagaattt tcttcttcgc agcatccaga gattcttttg ttgtgtattc
    18181 cgcttcatca ataaaggcat aacgcgttac ctgtttgccc tcaacttcct cctgccagct
    18241 agcaacttcc agtttcggtt tcttatcagg tttgttatca atctgatatt tccaggtaac
    18301 ttttcctgtt tgggaatcaa tggtgtacgg cagctcacct tccacaaccg gataacgttc
    18361 ataaacccag atgccagtcg ctcgacgctg acgaacgcgg ttcggatacc cttgcggatc
    18421 gttatttact accgacttgt tcagtgccat cgacagacct gcggcatcca acagacgcac
    18481 aaaaccagac gcgctctctt ccttaagatt gctcatcacg ttttccatga tcagcaccga
    18541 tccacctttg ttcagatagg cgatcagatc ggtcacatcc tgctgagtca gcttcggttt
    18601 gctggtatct gcacgcagcg ggattgcata aggatcgtta cccacctgag tcacatattc
    18661 aaagccgtta aggatcagca gcggcatttc ctgcggatcg agatcgccat agctacttaa
    18721 atgctcaaca gagatgcccg caaaatccgg atggaagtcg aacgcagcgc tgtttcctgt
    18781 aacctgacca tgacgtttga aatagacagt atccaggttg gtgcctacgg tcatgctggc
    18841 tttcgcgtcc ggcgtccatt tatcgtcgga cagatagcgc agcacgttct ccatgaagtt
    18901 cttcatgtca tccgggtcgc tgttgagcgt acactgccca tctttattaa cgcccccgtt
    18961 ccagctgtaa ccgttcgggc aacgcaggat gctgttgtag tgtgggttac cgataaccat
    19021 cagcttgcca tcgcccactt gccccagcga aataaacggc aggttaaagg tggcggtatc
    19081 gcgcgtaacg ttttccggct caacaagaga aggcgcttcc gtaatgtacg ccagctcgtt
    19141 tttatcccag gcgcgttttt cgccaaacgc cagccagtag tttttatcat tacgcgccat
    19201 cagaatcggg aaggccgcgt tggagatatt caccaccgcc tgaccgcgcg cattacccgt
    19261 gctgccatag aagttagtag agtcatggaa tacatggaac ttgctgacag atttgtagtt
    19321 cgtatccacg ccccacagct tgttaataac gccctgaatc tggccgtcat taacattgcg
    19381 cgtcgtcagc gagaaccagc gagcctcgtt acaaccgtcg gttttcgcac aaatcgcggt
    19441 atcgatctct ttggcctgac ccgtcttaaa ctgctcgata aattcgttag gcagattaac
    19501 gacttgctca ccttccccca gcgtcgcacc attggataac gagagattga taatctcgtt
    19561 aatcacgttg ggatattcag caaaaacctt gcgtacttca tccggaacta cacgcgtgtg
    19621 attttgtcca gccttcgaat agcggtgaat caactgatcg atatttgccc cgcgaacttc
    19681 atcacccagt tcagtcaatg caatagtcga cttgttacca cgcacagaac caagctcaaa
    19741 agtgtcgatg ccaaaggaga tggtttcccc ccaactaaag gaaaattccc cgttttctcc
    19801 ggttacacca cggccggaat tggtgtaata gttgacacca acaacaccat ccccctgact
    19861 atcgaccagt cgaccttcag agagaatgat ttcagtgggt tgatactgat aaaactgttc
    19921 cgcgttagcc gacacgaagg aagcgtttag atccggtttt gttcccggag tggtggcggg
    19981 cacgaccggt gaagtatgag tggatggcgc tttatcggtc gcggcattgt tttccacctc
    20041 ttcattgacc agctttttga attcttccgg tgccagatcg atttgcttat acagcgagtc
    20101 gaaacgttta ctctcgatca ccgaggagaa ctccaggcac acctgttctg tattcgccgg
    20161 gcaactgttc atggaggtga ccagcgagag cgcattgctt ttcttgttgt cggaacccgc
    20221 cagttcttgc gcgtcctcaa gactaaacga cactttttca accgcacgca ggctacgcgc
    20281 agcttctgac tgggtgtcga aggtagcaat tgtcgtgttg ttccctgcca cacaggtgac
    20341 tttgtcgcct ggtgtaaagg taaagccatc gctggattca tcattacagg tgatatcacc
    20401 agttacccgc aggcttccgc ccagagtcag ataacccgtt ttcgtaggaa caggttctgg
    20461 ctccggttct ggtgttggct caggatcagg tgtcggttcc ggcgtaggtt ctgggtccgg
    20521 cgttggctca ggcgtcggct ccgggtttgg tgttggatca ggtttcactt ccggcaaaga
    20581 ccctgttcca gaatctacag gcggcgtatc ggaggaagat ccggagccac cgccatcaca
    20641 accggctaac agggttgcac tcaaaat
//
LOCUS       DAMZII010000085        20512 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZII010000085 DAMZII010000000
VERSION     DAMZII010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.085"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            222..977
                     /gene="repE"
                     /locus_tag="POA77_003037"
     CDS             222..977
                     /gene="repE"
                     /locus_tag="POA77_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDI6366428.1"
                     /translation="MAEIAVINHKKRKNSPRIVQSNELTEAAYSLSRDQKRLLYLFVH
                     QIRKSDGSLQEHDGICEIHVAKYAETFGLTSAEASKDIRQALKGFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGVVSLKIDWIMERYQLPQSYQRMPDFRRRFLKASVDEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTIE"
     gene            1355..2052
                     /locus_tag="POA77_003038"
                     /pseudo
     CDS             1355..2052
                     /locus_tag="POA77_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2462..3535
                     /locus_tag="POA77_003039"
     CDS             2462..3535
                     /locus_tag="POA77_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366429.1"
                     /translation="MRKSYTYGIPFGLHRETGRFLDITEVSRGSACNCICPGCRTDLI
                     ARQGEVKLWHYSHSTDLLGDCDGLMEAIRGKIIEIIQEKQLLGFPHLLAGYDGGTVPL
                     DEVSGSGSMFGATADLFVKVNDLCVAVFLDIDRSVAGKLTFDHLHASEMVAALRIDLP
                     DIEYEISQVQLGRRDGTYSECIEKIIIDATESREWLYHPMMQELGTEPLKVYEGREPA
                     EAGPLLQRLSDCPLKLLDNLPQSMNVLIRMQQATIVCLCRLTVRASDIAQTDEFPLFM
                     RYFRLHCLDTSEHVNYEKLLNWQDMITKSKEGQLLTVEERDYLQEIVRIGLYNHRLKS
                     VCLTACEDSRVGESEGPNNYSLL"
     gene            complement(3792..4268)
                     /locus_tag="POA77_003040"
     CDS             complement(3792..4268)
                     /locus_tag="POA77_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366430.1"
                     /translation="MEKTQSPAERFYESIYYSDESLEEDYLAELRNFSSDHWDTALRA
                     ARLSAAVKRFKTSEMLRFILEFVVPENAQEDAPDLTPLAAKRLCNSLFGRSGSQSILV
                     YVFGQAGRVHRSATCSPKTIEAIAALYRSDAERYWNSTLATIERVKHTYRAKIRNS"
     gene            4939..5331
                     /locus_tag="POA77_003041"
     CDS             4939..5331
                     /locus_tag="POA77_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366431.1"
                     /translation="MDDKEQFTSLVAKHASRFTEEQLAGYDACSQYGECVSPSYEVFR
                     GYRTRHTLDEFLELAISLNAIHPDEYLTDMLLKPHEVIGALADEGDQLNNATPVYFFP
                     DTGVYAAAVSETRVLDAWLCWPCYPANW"
     gene            5744..5947
                     /locus_tag="POA77_003042"
     CDS             5744..5947
                     /locus_tag="POA77_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366432.1"
                     /translation="MFSTEKKSEHLGQIIVIDPALLGQGIEVGPVVVFDLGHASKGVA
                     DLMRDAEKLDGLSPTTIANRLSR"
     gene            6102..7307
                     /locus_tag="POA77_003043"
     CDS             6102..7307
                     /locus_tag="POA77_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366433.1"
                     /translation="MRSEIPSGRFFCLDEFHNFHMSDDVLRERLRSIDGIPAVSYQVN
                     GENVFVFEKLVQLVKQNGHNLESALALVPKATMTAAEAIEAWSVFPEPYSRETNTIDH
                     SQAIYHPYLNAYLPRKYPDRESCNADAARLLLGNPVEGEGDFVYRGQSMVLRQPFTLD
                     RLPPRPEWDVPVSEDASAYTHLWNTDFVSGFAPVTESLFNGIKSAEWEWNHAIAATYA
                     YMGNPECISSLWSRAGEFGLEQLELKSWDVDEQDPEHYQQFQALYPELSMLSPAIIQK
                     AYDEYCESMWENPERCDGFLYHLIGSTVSSDISRDNPEKTGRLIAHALLTGASIEDAC
                     RFASEAQQFDTALSGLAYQVAVAMKFVAGDSAAVRNEGHPVITLSDSFRLMRKANINT
                     LTATQNNRK"
     gene            7477..7968
                     /locus_tag="POA77_003044"
     CDS             7477..7968
                     /locus_tag="POA77_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366434.1"
                     /translation="MKLTKMMATLFLLIPLTSFSQELGQRISQDDSAELANKAGTALT
                     EMLLLKVQQLPQVKKNSGMDLAAANDAVASLLSKEFFIFKPVDEISIKTSAQKLKELN
                     DDADALAKGYTIIQPDDVGSSYISYKVTSSSRTCFIRLSKDLSSSLNTRIVTVNSMEC
                     SNP"
     gene            8070..8297
                     /locus_tag="POA77_003045"
     CDS             8070..8297
                     /locus_tag="POA77_003045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366435.1"
                     /translation="MIIILGVLLLLSLFFNIWFWDHYMRVIPLSADKSSMFAIASSCE
                     NPRWVQEVESRGGMTRKEWADFVDRNFNPPK"
     gene            8407..9213
                     /locus_tag="POA77_003046"
     CDS             8407..9213
                     /locus_tag="POA77_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6366436.1"
                     /translation="MREPRPRHTLQVVCVPSLNIQDLGLTSFHSWLLSKGYDTTNARN
                     NRTCWAREGGWHLKRCRHLATGEDHFWFIAFNGTGGDIFPVKTQQDYRAAYRKLEAYG
                     YAPAVIEQLNTGVAYNLAYSRTPLKRPESATSELKRKPDVNIQGEPCERFVTERSSAA
                     QNKFKTLLIENFAGRCAVTGWVNGGVLVAAHIEHGTRYNPSNGILLTPTMHALFDADL
                     MGIDPSTLTVHFKPGIEAGELFEGKTLNPLVYDLDTDRLAARWADFIGAE"
     gene            9331..9738
                     /locus_tag="POA77_003047"
     CDS             9331..9738
                     /locus_tag="POA77_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366437.1"
                     /translation="MQNCLSGYLKATIVILSAGLTGCDLANGTAESGVYQKYTNGPFK
                     EPTIVMSPGWRMDDHGREALIFGSSHCPDVNGNNTSEGGCVLIEEHSATVAVTVVDAT
                     NQSRRHETWTIERKKDRVIVKRPDNSFVMPWGK"
     gene            9785..10222
                     /locus_tag="POA77_003048"
                     /pseudo
     CDS             9785..10222
                     /locus_tag="POA77_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(10777..11181)
                     /locus_tag="POA77_003049"
     CDS             complement(10777..11181)
                     /locus_tag="POA77_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDI6366438.1"
                     /translation="MSEALKSLNNIRTLRAQGRELPLEILEELLEKLSVVVEERRQEE
                     SSKEAEQKARLEKIESLRQLMLEDGIDPEELLSSFSAKSGAPKKVREPRPAKYKYTDV
                     NGETKTWTGQGRTPKALAEQLEAGKTLDDFLI"
     gene            complement(11443..11715)
                     /locus_tag="POA77_003050"
     CDS             complement(11443..11715)
                     /locus_tag="POA77_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366439.1"
                     /translation="MNRTDDSITQLSTLYERLGAVAHELEYCKILLPEHSEQLDLDAA
                     ATYAMMLRIEQWAANVKNAYNTNTYDPFHINEKDKQLRNQEEENQY"
     gene            complement(11712..12380)
                     /locus_tag="POA77_003051"
     CDS             complement(11712..12380)
                     /locus_tag="POA77_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366440.1"
                     /translation="MAASRITHLITSCTKGKHSQCGSMPELSIRSGQTPEEAMSSWAA
                     TIKRSQSASPVPALSLYAGNHWSTAKEILRTTENLELWVISAGLGFLNSRDLVDAYEA
                     TFHDLPFSHRQWWRELTNTFGKERTAKSIETLMAARPFDDYVIAASPVYIEATEDDIL
                     AGASKLNNHIAQLTVVTSGEYSGLLESYLIRSESRMMRQLSSNMVCLNIKLAQYIIGS
                     RRYS"
     gene            12887..13342
                     /locus_tag="POA77_003052"
     CDS             12887..13342
                     /locus_tag="POA77_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6366441.1"
                     /translation="MSMNSQPELKLSTRTEQLASSRDAAMQKFLDGMTLIAEASAICG
                     FSLFNSKIMAPNAFGLPASLAASIEEGRQQIDRKTWNNLFEETGIDRFWNHNQRAEFR
                     ESLRNAPPIASLTVIRSTLRQAVAMRSITLAEGFVDLLCQLDRRYKTNA"
     gene            13511..13699
                     /locus_tag="POA77_003053"
     CDS             13511..13699
                     /locus_tag="POA77_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366442.1"
                     /translation="MYDRLAVLRKTDFTGDITDPKGWKFRLFGNGNVHISVECESLHN
                     ALNDLISIYFANQIPAKG"
     gene            13705..14922
                     /locus_tag="POA77_003054"
     CDS             13705..14922
                     /locus_tag="POA77_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366443.1"
                     /translation="MTDNVNLMNGDEQTASSLSEETSKTPPLSPELLKNSPYSSIAKV
                     KYFTEIEQICLDKSISTENLDQFFKAHWLRDKMGGSFARAQEMLAAYKQYVDEVPEEA
                     RAIEIPDQIKDAFSDFTAFITWYFRLSYTAIQSDSVKIAKAEITQLRQRNAEILEELS
                     QSKEQATVLNNEKVNLINLLEQQRELSSKLEDSLQEAEETLAGTQSELQHAQNEIQLL
                     QQTVGTLNQQLSERKQELASQQEYQKQLNDENKAQQVELTALKSQNDHLQRTVSDLKV
                     SVSQLEQDLSSVQTHSSELSSSLAEKDTTLTLVRSELSTAKGENDQLRAEAQRLADES
                     LVAKKVQTDQTEELQQLRNQMISMEATLNAEKTIAESLRGTIKQLTEAMTGAVAIKPK
                     SSGASKPRNKKTT"
     gene            complement(14977..15180)
                     /locus_tag="POA77_003055"
     CDS             complement(14977..15180)
                     /locus_tag="POA77_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366444.1"
                     /translation="MAYILSMSEQVKLKAFLAAVLDDYKLGAISQQQAVGGITSLVDA
                     IEISDSGKISLMLSEGRKLLRTG"
     gene            complement(15218..16513)
                     /locus_tag="POA77_003056"
     CDS             complement(15218..16513)
                     /locus_tag="POA77_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HDI6366445.1"
                     /translation="MGQKFAVFIAYDDEPNTKRYSAEFQTQDEYVKGWQSALKKAHHT
                     SGQKSVISCGCRGKGAKRLYVRSLPNSDTFILIKAANTGTEHDPSCVFFDLDARHTGL
                     KGYASNVVRINNEGTMSIRLGIGMTEKDPPEKSEVPSLPQIQRPEGGQASMTLSGLLS
                     LLWTEAGLNVWYPNMAGKRNDSLVRYRMLEAAKQIRSGRACIGDHLFIGVADSKSKVA
                     SEQVQLLSSAELSDKRLLLLSVLPRYDAEKHEKPLKFLPMRNFGGMPLTFFNSDGHWD
                     SVKRRFPLEYAAWKNGGKVVVFALTSPVSVTSRGISARAHQIVLMLVSDNWIPLDSSY
                     EAIVSRKLDAEHRHYVKPMRYDASASDVFPDFYLLDTRSDKPFPMEVFGMSTPAYLAR
                     KELKKAYYNQEYGAYGWWHWDATEKTEYQELPHFPEARR"
     gene            complement(16538..16708)
                     /locus_tag="POA77_003057"
     CDS             complement(16538..16708)
                     /locus_tag="POA77_003057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366446.1"
                     /translation="MMTYFDSAEDLTISKQRALQELAKHGVVASDIDVFFSELGEREE
                     YNAQEVLIWLGY"
     gene            complement(16837..18228)
                     /locus_tag="POA77_003058"
     CDS             complement(16837..18228)
                     /locus_tag="POA77_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDI6366447.1"
                     /translation="MLNDETHSSIHKLLVQVASIYDVKDLAAQLNAATGSDWSRASLI
                     RQIKGSVNECRITQEEYHYLRSLLPSRPADYDQKFFRFIDLFAGIGGLRSGFDAIGGK
                     CVFTSEWNQFSRRTYSANWYCDETEHYFNSDIRDITLSNLPDVSDDQAYASIDASIPD
                     HDVLLAGFPCQPFSIAGVSKKNSLGRKHGFECDTQGTLFFDVARIIRAKQPAIFVLEN
                     VKNLKSHDKGNTFRIIMKALDELGYDVADCDAHGSKDPKIIDGKNFRPQHRERIVLVG
                     FRRDLKLHDGFSLSGVSALYPSRRPTLGELLDAEVDSRYILSPKLWEYLYNYAKKHQA
                     KGNGFGFGLVNPLDKSVVCRTLSARYYRDGSEILLDRGWDHSIGEKDFNDPVNMARRP
                     RRLTPRECARLMGFEQPGKVTFRIPVSDTQAYRQFGNSVIVDVFAAVASLLRSRIEIA
                     VAARLKGEFEAAS"
     gene            18487..19002
                     /locus_tag="POA77_003059"
     CDS             18487..19002
                     /locus_tag="POA77_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6366448.1"
                     /translation="MTLLKKLLLPTLIVLFPAAVLAGQSSFEAKVVKIIDGDTITALD
                     GQNTSIKIRMYGIDAPESKQAFGQKAKQALSSAIAAQNITVIDHGPDIYGRMLGTIWL
                     DGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQ
                     NQKPQKTKSRG"
     gene            19008..19946
                     /locus_tag="POA77_003060"
     CDS             19008..19946
                     /locus_tag="POA77_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366449.1"
                     /translation="MTSQHPLLKIALNGDGLLSWPNSPGQTMRCPTCMRDVITGPDGD
                     LIHQMEEGANPCTPSVDVVISKAIIELLSTGERLYVNPVKNANRTLAPSFIFLHKDKQ
                     LRPFINTEYQPAGATWRSDKGNRLGFFYMDERASKINKDQFDFIAVIDPSTFQAEFKT
                     LWSMCEFDNPLAALQHLLASENMSSVWVKWPVKSLQPRKEVVKADYWYDYYSPHYPAQ
                     QAACTATVIGLEGNVSGETIYMLQIAVDRSIVEYQLVKSLGVILLLDNTGNLVSETHP
                     HFDIIIKACISGVRDFVRQNPRMRSVEHQITSAMPG"
     gene            19976..20167
                     /locus_tag="POA77_003061"
                     /pseudo
     CDS             19976..20167
                     /locus_tag="POA77_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5487 a         4734 c         4929 g         5362 t
ORIGIN      
        1 cctttatcca tgcgggttcc agagagggat ctgtgacaaa acgccctttc agtgtgacaa
       61 atcaccctca aatagccgcc ctttctgtga caagttgccc ttaaccctgt gacaaattgc
      121 cctcaggaag cgttgctttt cacaaagtta tccatccctg ttgactctgt tttatgaagt
      181 gtgacaatct taaagtctgt cacacttcac atggacctgt catggcggaa atagcggtta
      241 taaaccataa aaaacgtaaa aatagcccgc ggattgtcca gtcaaatgag ctgactgagg
      301 cggcatatag tctctccagg gatcaaaagc gtctgctgta tctgttcgtt caccagatca
      361 gaaaatccga cggctccctg caggaacatg acggcatctg cgaaattcac gttgctaaat
      421 acgctgaaac attcgggttg acctccgctg aagccagtaa ggatatacga caggctttaa
      481 aaggttttgc gggtaaggaa gtggttttct atcgccctga agaggatgcc ggcgatgaaa
      541 aagggtatga atcctttccc tggtttatta aacgtgcgca cagcccatca agagggcttt
      601 acagcgtaca tatcaaccca tatctgattc ccttcttcat cgggttacag aaccggttta
      661 cgcagttccg gctcagtgaa acaaaagaga ttaccaatcc gtacgccatg cgtttatacg
      721 aatctctgtg ccagtaccgt aaacctgatg gctcaggtgt cgtgtccctg aaaatcgact
      781 ggatcatgga acgctaccag ctacctcaaa gttaccagcg tatgccggac tttcgccgcc
      841 gtttcctgaa ggcaagtgtt gacgagatca acagccggac accaatgcgc ctttcttaca
      901 tcgagaaaaa gaaaggccgc cagacgacgc atatcgtatt ttccttccgt gatataacct
      961 ccatgacgat tgaatagttg agggttatct gtcacagatt taagggtggt tcgtcacatt
     1021 tgatctggaa ggttgagggt tatctgtcac agatttaagg gtggttcgtc acatttgatc
     1081 tggaaggttg agggtgattt gtcacagatt taagggtggt tcgtcacatt tgatctggaa
     1141 ggttgagggt gatttgtcac agttttgctg ttccctccct ggcatcctgc gcttactcat
     1201 ggttttttgg ctgtaaccgg tagtggagcc agaattgagg gcaatttgtc atcctatcct
     1261 gagggtagtt tgtcacacta gattttcccc ctcttcccgg gtaatggtgc caacttactg
     1321 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
     1381 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
     1441 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
     1501 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
     1561 cgcccgcatt atgggcgttg gcctcaacac gattttccgc catttaaaaa actcaggccg
     1621 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
     1681 gaacagtggg gttacgtcgg tgctaaatca cgccagcgct ggttgtttta cgcgtatgac
     1741 aggatacgga ggacggttgt ggcgcacgta ttcggtgaac gcacgttggc cacgctggag
     1801 cgtcttccgg gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tgactggccg
     1861 ctttatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
     1921 attgagcggc ataacctgaa tctgagacag catctggcaa ggctgggcag gaagtcactg
     1981 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaccataaaa
     2041 cactatcagt aagttggagt cattacccag taatgcgaca aatgcaatga aagaagtagc
     2101 gaaagtcctt ttttcagccc gaagtgaccc ggagctcatg aaatttatga tggattatat
     2161 taataaccac aaactgcatt gaaggtaatt gcggtgtata aaacaaagcc attttcggcc
     2221 aaaaaatgag tcagctgaag aaacgctcgt taattccgaa tccgtccaga ttaatatggc
     2281 acactgctgg tggaggtttt ggcaatcgga aaagtaactt ttcacccgtg tctaatcatc
     2341 atcgttttat caatccgctc agttacggta ttaaaatctc acgaaaaata gtcttcttat
     2401 cgaagtgtca tgctgtcttc gcccgatata gtgaaggtat gtattcagcg ggggcactga
     2461 catgcgaaag agctatacct atgggattcc tttcggactc catcgcgaaa ccggacggtt
     2521 tctggacatc acggaagtca gcagaggaag cgcatgcaac tgcatctgtc ctgggtgtcg
     2581 tacggatttg atagccagac agggagaggt caaactttgg cactatagcc acagtaccga
     2641 tctcctcggt gattgtgatg gcctgatgga ggcgattcgg ggaaaaatta ttgaaattat
     2701 tcaggaaaaa caacttctag gattcccgca tcttctggcc ggttacgatg gaggcacggt
     2761 cccacttgat gaagtcagcg gcagcggaag catgttcggc gcaacggcag atctgtttgt
     2821 gaaggtgaac gatctctgcg tagctgtgtt tctggacatt gaccgcagtg tggccgggaa
     2881 actgacgttc gaccatctcc atgcctcgga gatggtagct gcattgcgca ttgatctgcc
     2941 ggatatagaa tacgaaatca gtcaggttca gcttggtaga cgagatggta cctacagtga
     3001 atgtatcgag aaaatcatca ttgatgcgac ggagtcacgc gaatggttgt atcaccccat
     3061 gatgcaggaa ctggggaccg agcccctgaa ggtatatgag ggccgcgagc cagcggaagc
     3121 aggaccactt ctacaacgcc tttccgactg ccccctgaag ctgctggaca acttacccca
     3181 gagcatgaat gtgctgataa gaatgcagca ggccaccatt gtgtgtttgt gtcggctcac
     3241 cgtcagagct tcagatatcg cccagaccga cgaattcccg ctgtttatgc gttacttccg
     3301 gctgcattgc ctcgacacca gtgagcatgt gaattacgag aaattgctta actggcagga
     3361 catgattaca aaatcaaagg aagggcagtt actaacggtg gaggaaaggg attatctcca
     3421 ggaaatcgtc cgtattggtc tctataacca ccggcttaaa agtgtctgcc tgacggcctg
     3481 cgaagacagt agggtgggag aatctgaagg accaaacaat tactcattgc tctgaagagg
     3541 gatgggaaat tatgggggga catggaagcg tgaagagaat aaacctgttc gccttaaaca
     3601 gaaacaggct gttcatttct tacatgtgaa cagagtacct atcatgatca gaccggtaat
     3661 ataaattggc acagccatca aaaccaatct cagtggttct tctttggtaa aaacatcctg
     3721 aataagtccg tcttcgctta aagctaatgg acgcatacat tagctttaag gcgtaaggtc
     3781 taataactgg atcaggagtt tcggattttt gcgcggtagg tgtgtttgac cctttcaatt
     3841 gtcgcaagag ttgaattcca gtagcgttca gcgtcagaac gataaagagc agcgatagcc
     3901 tctatggttt tgggggaaca ggttgcactg cgatgcacac ggcctgcctg gccaaagaca
     3961 tagacaagaa tggactggct accagaccgg ccgaacaggg agttgcaaag ccgcttcgct
     4021 gcgagcggtg tcagatcggg ggcgtcttcc tgagcgttct cgggaacaac gaattccaga
     4081 atgaagcgaa gcatctccga tgtcttgaaa cgctttaccg ccgcagacaa acgggcggca
     4141 cgcagtgcag tgtcccagtg gtcgctgctg aaattgcgga gctccgcgag gtaatcctct
     4201 tccaggcttt catcagaata ataaatgctt tcgtaaaagc gctctgcggg tgattgcgtc
     4261 ttttccattg gagaaaccat tttgcctaat gtatgcatac gttagcatgt agtgaaatta
     4321 aaacaaggta aagacaaaga accgcattca ggcgtttccc ggagtaagca ggtgggctga
     4381 caattcggct gcggtaatat caagagctct gttaattgag cgttaaatgc ctcccgctcc
     4441 agtccggatt ggatgagaaa gatcagggcc tttttcagaa cgggcggaat actcgcaatg
     4501 tcgaggagaa tgttgccctc aataacatga tcgcaatttt caaaaaggtg ggcgtaactc
     4561 attacgtgta ttccatttat cattttcaaa tcgtgataaa aattagatta ccagaacgtc
     4621 agaagcaaaa aaaaaagcat taagcagaaa tgtatggtat ccatccttcc tccaggccct
     4681 gataatttta tcctggggga gggggcattg atcattacat acccgatcag aaaagccggg
     4741 aaccagaatg tcacccggat atcgaagatg caacgattca atattagtgt atttagcgtc
     4801 tcctggcagc aaagtcggtc ggccggaact ggtaaactgt attccacctc gcccgggctg
     4861 gcaggcgact gacgtcttgt cggttcatag ccggtggtcg cgtctgacaa aacaggcccc
     4921 tacagtgtaa ggattgtgat ggacgataaa gagcagttta cgagtctcgt ggcaaagcat
     4981 gcctccagat tcaccgaaga gcagctggcc gggtatgatg cctgttccca gtatggagaa
     5041 tgcgtctcgc cttcatacga agtattccgg gggtatcgta cccgccacac cctggatgaa
     5101 tttctggagc tggccatatc gctgaatgcc atccacccgg atgaatattt aacggatatg
     5161 ctgctgaagc ctcatgaggt gatcggtgct ctggccgatg aaggcgacca gttgaacaac
     5221 gccaccccgg tttatttctt cccggatacc ggcgtctatg cagcggccgt cagtgaaacc
     5281 cgtgtgctcg atgcctggct ttgttggcca tgctacccgg cgaactggta atgcgtccgt
     5341 tggaattgca ccggcaggat acgaatttaa ccgaccccgt ggcgggaggg ttatcacaat
     5401 tgcatgtcct catccctaaa gtcccctgcc agacccatac gtgctgtcag gcttcgcctg
     5461 atttgagatc agtgcaaata cttcagccga taactttttg ttttcttttt attttctccc
     5521 tgtttcctca aaattcgcgt gcggcctggt cagggcacga ttgttacata gggtgtattg
     5581 aagcgggcag gatggtcggg ttggcacggc ctgggtttga gtcggacact atgtgggatg
     5641 aggacatgca aaatattcgc tttatctgtg aaaactgttg tctccgggca gtcaccagtt
     5701 caagttacac catccacgtt agcttttaat aagagtggtt acgatgttca gtactgagaa
     5761 aaaatcagag catttaggtc agatcatagt gattgatccg gcgcttcttg ggcagggtat
     5821 agaggttggc ccggtcgtag tgtttgattt ggggcacgcc agcaaagggg ttgcggactt
     5881 gatgcgcgat gctgaaaagc ttgatggttt atccccaacg acaatagcaa acagactttc
     5941 acgatgaccg aacaaggtcg attcgggcaa ggcggcggag gggcacatct cccaatgata
     6001 atgaggttat gcaacctgtt agtcaccggc ttgcctgatg ctatgattga catctctttt
     6061 gaccggtctg tatttgagca ttaattcgtg aggaatacgg tatgcgcagt gaaatacctt
     6121 ctggtagatt tttttgtttg gatgagtttc acaatttcca tatgagcgat gatgtccttc
     6181 gtgaaagact ccgttcaata gacggcatcc ccgctgtgag ctatcaggta aatggcgaaa
     6241 atgtttttgt gttcgagaaa cttgtccagc tggtaaaaca gaatggccac aacctggaat
     6301 cagccttagc tctcgttccg aaggcaacaa tgaccgccgc agaggctatt gaagcgtggt
     6361 cggtattccc cgagccgtat tctcgtgaga ccaatacaat cgaccatagt caggcgatct
     6421 atcaccccta tttaaatgcg tatctgcccc ggaaatatcc cgaccgtgaa tcctgtaatg
     6481 cggatgcggc cagattactt ctcggcaatc ctgtcgaggg tgagggggat tttgtctaca
     6541 gggggcagtc catggtcctt cgccagccct ttaccctgga ccgtcttccc ccacgacctg
     6601 aatgggatgt gccggtgtct gaagatgcca gtgcctacac acatctctgg aacacggatt
     6661 ttgtcagtgg cttcgcaccg gttaccgaat cactgttcaa cgggataaaa tcggcggagt
     6721 gggaatggaa ccacgctatc gctgcgacat acgcatacat gggaaatccg gagtgtatct
     6781 cttcgttgtg gtcacgggcg ggtgagttcg gactggagca gctggagctc aaatcctggg
     6841 acgttgacga gcaagatccg gaacactacc agcaattcca ggctctttac ccggagctgt
     6901 ccatgttgtc cccggcaatt atccagaaag cctatgacga gtattgtgaa tcgatgtggg
     6961 aaaaccctga aaggtgcgac gggtttctgt atcaccttat tggcagtacc gtcagttcgg
     7021 atatctcccg cgacaaccca gaaaaaaccg gcagactcat cgctcacgcg ttactgacgg
     7081 gggcctcgat tgaggatgcc tgtcgctttg cctcagaggc tcagcagttc gataccgctc
     7141 tatcgggact ggcttaccag gtagccgtgg ccatgaaatt tgttgccggt gattctgcgg
     7201 ccgtaaggaa tgaaggccat ccggtcatta cactttccga ctccttccgt cttatgagaa
     7261 aagcaaatat taacaccctg acggccacac agaacaaccg gaaatgatga gcacaaatcg
     7321 atatggatgc cgatattaag aacgatgatt atgttgactg tctcattgag caggtaaccg
     7381 tatagcggac tggaacacga atgctgatgc tctacaccag gcctgcggcg ccttcgtaga
     7441 tgcaccgcat tcaaaacaaa cgacaggtga taatttatga agttgacaaa aatgatggcg
     7501 accttgttcc tgcttattcc cttgacatct ttttcgcagg aattgggcca gcgaatatca
     7561 caggatgaca gtgcagaact ggcgaataag gcaggtacag ctttgacaga gatgcttttg
     7621 ctaaaagttc agcaattgcc acaggtgaaa aagaacagtg gaatggacct tgcggctgca
     7681 aatgacgctg ttgcttcctt actcagcaaa gagtttttca tttttaaacc agttgatgaa
     7741 atcagcataa aaacctctgc tcaaaaatta aaagagctga atgatgatgc cgacgcactt
     7801 gctaaggggt acaccatcat tcaaccagat gatgtcggaa gctcctacat ttcgtataag
     7861 gtgacgtcca gctctcgaac ctgcttcatt cgcctttcaa aggatctgtc atcgtcatta
     7921 aataccagaa ttgtaactgt gaatagcatg gagtgttcga acccataaac cacgttgacc
     7981 ggctaattca ctttacgggg taaatgagtt gtgaaaccca ttttattttc accctcaatt
     8041 acagaaattc aagccgaata gagactgtaa tgattattat ccttggtgtt ctgcttttat
     8101 tgtctctttt cttcaatatc tggttttggg atcactatat gcgtgtcatt ccgttatcag
     8161 cggataaaag tagcatgttt gctatcgcct ccagctgtga aaaccctcgg tgggtgcaag
     8221 aagttgagag ccgtgggggg atgacccgta aggaatgggc tgactttgtg gatcgaaatt
     8281 ttaaccctcc taaatagagt aaattccgat tggcattgtt gcaggaatta tggtgccgca
     8341 gcttattggg tgtttgtcaa ttgccgggaa gtattcgctg gcatatcact taaacgggag
     8401 tagtaagtga gagaacctcg tccacgccat acattacagg tagtctgtgt cccgtctctg
     8461 aatatacagg atctggggct aaccagtttt catagctggc tcttatcaaa aggctatgac
     8521 acgaccaatg ccaggaacaa ccgtacttgc tgggcaaggg aaggtggctg gcacctgaag
     8581 cgatgcagac atctggcaac gggcgaagat catttctggt ttatcgcatt caacggtacc
     8641 ggcggcgaca tttttcctgt gaaaacccag caggactacc gtgctgccta ccgcaagctt
     8701 gaagcgtatg gatatgcacc ggccgttatt gaacaattga acacaggcgt tgcctataat
     8761 cttgcttatt cacggacacc gttaaaacgc cctgagagcg ccaccagcga actgaagcga
     8821 aaacctgacg tgaatattca gggtgagcct tgcgaacgct ttgtgaccga acggagcagc
     8881 gcagcgcaga ataaattcaa aaccctgctc attgagaact tcgccggtcg gtgtgcagtc
     8941 actggatggg ttaatggtgg tgtgctggtg gcggctcata tcgagcatgg gacgcgctac
     9001 aacccttcga acggcatcct gttgacgccg acaatgcacg cattgtttga cgcggatctg
     9061 atgggtatag acccttcaac actcaccgtt cattttaaac cgggcatcga ggcgggagag
     9121 ctgtttgaag gaaaaacgct caatccgttg gtgtatgatc tggataccga ccggctggct
     9181 gctcgctggg ctgactttat aggtgccgag tgattgatgg gaaattcagc ctataaccga
     9241 tggcagtgtt ttcgggatgg acaccactta ctgatggggg ctggctgcac gccaccttta
     9301 tattgaaatc caacagagtt gatacaacaa atgcaaaatt gcctttcggg atatttaaaa
     9361 gcgaccattg ttattttatc tgcgggcctg acaggttgcg acctggcaaa tgggactgcg
     9421 gaaagtggtg tgtatcagaa atacacgaat ggacctttca aggaaccgac tatcgtgatg
     9481 agtccgggct ggcggatgga tgaccacggc agggaagcac taatattcgg gagcagccac
     9541 tgcccggatg taaacggcaa taatacgtct gagggcggct gcgtcctcat tgaggagcac
     9601 tctgcgactg tcgcggtcac tgttgttgat gcgacaaatc agtcccgtcg ccatgaaacc
     9661 tggacgattg aacgtaaaaa ggatcgagtt atagtgaagc gcccggataa cagttttgtg
     9721 atgccctggg gaaaatagcc aagatacaag atgaccacaa aaatgaataa gccgcaatgt
     9781 tgttttgtgt gttgaacgtc ctgatggagc ctttgacatt tacactggcc gttttataca
     9841 gttccaccaa attgacaaca gtagaagaaa aattgttgaa ataagtgatc ctgacctggt
     9901 ggggatgact tattcaatcc cgatggtttt taacggaggg aagttcagtg gtcaaggggt
     9961 tacctgtctc tcgctccccg cagtaacatt aataaaagta acttttcctt cccgaactta
    10021 tcaggagggg caggaacaaa tcacaatgtc gagcccggac caggcactat tttctgaaga
    10081 tgtccggaaa aactgcgccg gtacgtgtgt tcttaccggc gttctaggca gacaaagaac
    10141 tgaggctgcg cacataaaac cccgtcatgc tggtggtgag ccggatgtga caaacgggat
    10201 tctactgcga agtgacatcc atattggaat ttactgcttg gcaggcataa taagaggcaa
    10261 tagagtaaaa atcccgaaag aacaagatat gccatgtacg cttcaattaa cgatgatata
    10321 tgtcggcatc acctttttcc taattgtcgg ttatatctgc catcaaataa cttagaactg
    10381 atttagaatt gaatttgata aagcttacta caaagtagtt ttgtcaatgt tcaacctgtt
    10441 tatcaaacct acggtgactt tttatgactg ggaatggtat caatactgta cgcataaaca
    10501 acgaagtgaa acacatctca gagttagatc cggagatctc tttttcttga atggaataaa
    10561 ctgaaaagtg aaaataacga actgtatcgc tgcataaagg aagcaaattc tggttggcgt
    10621 ggttttgtcc ttcgtttaat aggggtttat ctacctgatg gcaaaacaat tgtcattaaa
    10681 ccctagatta tttgaatatt cctaaacata ggcttaataa gaaaagccac tgaaaagtgg
    10741 cttttgaact gagctgttcg cgattttaat ttcaacttag atgaggaaat catccagtgt
    10801 tttaccggct tcaagttgtt cggccagtgc tttaggggta cgcccctggc cagtccaggt
    10861 tttcgtttca ccattaacat cagtgtattt atatttcgca gggcgcggtt cgcgaacttt
    10921 tttaggtgct ccggactttg cagagaaaga actcagtaac tcttcagggt caattccatc
    10981 ttcaagcatt aattgacgca gggattcaat cttctccaga cgcgctttct gctcagcttc
    11041 ttttgaagat tcttcctgac gtcgttcttc aacaacaacg cttaattttt ccagcagttc
    11101 ttcaaggatc tctaacggca gttcacggcc ctgcgcacga agagtacgaa tgttgtttaa
    11161 tgatttgagt gcttcggaca tatgacctca ctaagagtaa taaataaaat caatagaata
    11221 ctttgcgata ttacactctt ttattatttt gagccagcta gacgcgtaag attttctggg
    11281 cgcatgatat caatcattgg tacctaaaga cattaaccac cccgcgtatg gaaacaaatg
    11341 cattatcact tgccagttcc tctgtagtgc caactccgca agattagata aaatcgccgt
    11401 ctttctcatg cgattattac tttcggttac tggagagacc ttttaatatt gattttcttc
    11461 ctcttgattt cttagttgtt tgtccttttc gttaatatgg aaagggtcgt atgtgttggt
    11521 attatatgcg ttcttaacgt ttgctgccca ctgttcaatg cggagcatca ttgcatatgt
    11581 tgctgcggca tcaagatcca gttgttcaga gtgttcaggg agcagaattt tacaatattc
    11641 cagttcatga gccacagctc cgagccgttc ataaagtgta gatagttgcg ttatggaatc
    11701 atcagtgcgg ttcatgaata gcgccgactt ccaataatat attgagcaag tttgatgtta
    11761 agacatacca tgttgcttga tagttggcgc atcatacggg attcgctacg aattaaatag
    11821 gattcaagta gtccagaata ttcccctgac gttacaaccg tcaactgagc aatgtgatta
    11881 tttaatttgc ttgcccctgc caggatatca tcctccgtcg cctcgatata aacgggggag
    11941 gcagcgataa cataatcatc gaaaggtctt gctgccatca atgtctcaat agatttcgcc
    12001 gtcctttctt tcccgaatgt atttgttaat tcccgccacc attgtcggtg actgaacggt
    12061 aaatcatgaa aggtggcttc ataagcgtcc acgaggtcac gactattcaa aaaacccaac
    12121 ccggccgaga tcacccacaa ctccaggttc tcagttgtcc gtaatatctc ttttgccgtt
    12181 gaccagtgat taccggcata gagcgacagg gccggtaccg gtgaagcact ttgcgaccgt
    12241 ttaatcgttg ctgcccagct ggacattgcc tcttctggtg tctgtccaga ccggatactc
    12301 agttctggca tggagccgca ctggctgtgc ttacctttgg tacaggacgt tattaggtgt
    12361 gtaattcgcg acgccgccat tttgtagccc tcagccaaat gtgtgtaatc actgtctaac
    12421 tatcgtaaca gcccctcatc agccataacg acaaagagga agccctaaac gtaaacgact
    12481 accccattat acatttagtc atgcctttac taataactag ttaccaaaat aaacacccca
    12541 gaggtgacac cgtcacgttt ttcggcttta tttagttcat tttatgtact atgggattta
    12601 attcggggta aatcccatcg tacacatagc ccccctcata gtgtaattgc ggataaaaac
    12661 cttattaatc agtcatatag ctcaatggtt gtgcattcag ggaatgcgat cgccacttaa
    12721 cgggttgcgg gttgttggtc acccgacatc tgtgtgctaa tctttttcat cattagtcat
    12781 tagtaaaggc gttactataa atgacgctac gagaattttt ttaactagag aggtggatag
    12841 gccagtgaaa atctctcagc caaaacagta ggaaaaggat cgtgaaatga gcatgaattc
    12901 gcagccggaa ctgaagttga gtacccgtac cgaacaactc gccagtagtc gtgatgccgc
    12961 tatgcaaaaa tttctggacg gcatgacgct gattgccgaa gcctcagcaa tttgcgggtt
    13021 ttcactgttt aacagcaaaa ttatggcccc taatgctttt ggccttcctg cgtccctggc
    13081 cgcaagcatc gaggaagggc gacagcaaat agaccgtaag acatggaaca atctttttga
    13141 agaaacgggt attgaccgat tctggaatca caaccagcgg gcggagttcc gtgagtctct
    13201 ccggaacgct ccgccaattg cgtcgttaac cgtaatccgc agtacattgc gccaggcagt
    13261 tgccatgcgc agcattacgc ttgctgaagg gtttgttgat ctgctatgcc agcttgatcg
    13321 ccggtataaa actaatgcct agcaatttgt gatgccaaaa aaactggtac tacgcggcat
    13381 ttttcccgac tcgaacatga tgagatataa tggcttttct caggataatc tcttctattt
    13441 aaacgacttt gagaatgtcg tttgcatctg ctccaatgca gcaacaccgc ctgtcggcag
    13501 tggaatgaac atgtacgatc ggcttgctgt cttacggaaa accgatttca cgggtgacat
    13561 taccgacccg aagggctgga agttccgatt gttcggaaat ggtaacgtcc atatctcagt
    13621 tgagtgtgaa agcctgcata acgcactgaa tgacctgatt agcatttatt ttgccaacca
    13681 gatcccggcc aaaggataag aatgatgact gacaatgtaa atttgatgaa cggggatgaa
    13741 caaactgcgt ccagtttaag tgaagagact tcaaaaacgc cccctctaag ccccgagtta
    13801 ctgaagaatt ccccttactc gtcaatagct aaggttaaat atttcaccga gattgaacaa
    13861 atctgcctcg ataagagcat cagcaccgaa aacctcgacc agtttttcaa agcccactgg
    13921 ctgcgggata aaatgggggg gtcttttgcc cgggctcagg agatgctggc ggcctacaag
    13981 caatacgtgg acgaggtgcc cgaggaagca cgcgcgattg aaatccctga ccagattaaa
    14041 gatgcattca gtgattttac tgcgtttatc acctggtact tccgcttaag ctatacggct
    14101 atccagagtg atagcgtgaa aattgcgaag gcagaaatta ctcagttacg gcagcggaat
    14161 gctgaaattc tggaagaact gtcacagtcg aaagaacagg ctacagtcct gaataacgaa
    14221 aaagttaacc tgattaacct gctggagcag cagcgtgaac tctccagtaa attagaagat
    14281 tcacttcagg aagcagaaga aactctggca ggaactcaaa gtgaattaca gcatgcccaa
    14341 aacgagatcc agcttttaca gcagacagta gggacactga accagcaact gagtgagcgc
    14401 aaacaggaac tggcctcgca gcaggagtat cagaagcagc tgaatgatga gaataaagcg
    14461 cagcaggtag agctgacagc cctcaaaagc cagaatgatc acttacaacg gaccgtgtct
    14521 gatttaaaag tcagtgtcag tcagcttgaa caggaccttt cctcagtaca gacccattcc
    14581 tctgaattga gctcatcttt agccgaaaag gacacgaccc tgacgctcgt gcggtcggag
    14641 ctctcaacag ctaaagggga gaatgaccaa ctacgagctg aagcacaacg cttagctgat
    14701 gagagcctgg ttgccaaaaa agtgcaaaca gatcagacag aagagcttca acagctcagg
    14761 aatcagatga tttccatgga agcgacactc aatgcggaaa agaccattgc cgagtcttta
    14821 cgaggaacaa tcaagcagct taccgaagct atgaccggcg cagttgccat caaacctaaa
    14881 tcctctgggg catcaaaacc gcgaaacaaa aagacgacat aactgctccc gccaggaatt
    14941 tgcaatagac ttaaattcct ggcgtccaca ggctctttat cccgttcgca ggagtttacg
    15001 tccttccgac agcattaggc tgattttccc cgaatcactg atctcaatag catcaaccag
    15061 gctagtgatc ccgccaacag cttgctgttg tgaaatagct cccagtttgt agtcatcgag
    15121 tactgccgcc aaaaacgcct tcagtttaac ctgctcactc atcgaaagta tgtacgccat
    15181 ttttagccca attttaaggt tgtgattcgg gttaatttta tcgtctagct tccgggaaat
    15241 gagggagttc ctggtattca gtcttctccg tagcatccca gtgccaccat ccataggccc
    15301 catattcctg gttgtaatac gccttcttaa gttcctttcg cgcaagatag gcaggtgtcg
    15361 acatcccgaa aacttccatc gggaacggct tatcacttcg tgtatccagt aaataaaaat
    15421 ctgggaaaac atcgctcgca ctggcgtcat accgcatagg cttaacatag tgccggtgtt
    15481 cggcatcaag tttacgggac actatcgctt cgtaggagga gtccagaggt atccagttat
    15541 cactcaccag cataagcact atctgatgag ctcgtgcaga gatacctcta ctggtcaccg
    15601 atacgggaga tgtaagagca aaaacaacaa ctttgccccc atttttccag gcggcatatt
    15661 ccagagggaa tcgccgcttc acgctatccc agtgcccgtc tgagttaaag aaggtaaggg
    15721 gcataccgcc gaagttacgc ataggtagaa acttcagcgg cttctcatgc ttttctgcgt
    15781 catagcgcgg cagtactgat agcagcagca gtcgtttgtc gctcagttcc gccgacgaca
    15841 gcagttgaac ctgctcactg gccactttgc ttttagagtc cgccaccccg ataaaaagat
    15901 ggtcgccaat acatgctcta cccgatctga tctgctttgc ggcttccaac atccggtatc
    15961 gaaccagcga atcatttcgc ttcccggcca tattcggata ccagacatta agaccggctt
    16021 cagtccacaa caaactcaga agtccggaga gggtcattga agcctggcca ccttcaggtc
    16081 gctggatttg gggcagggaa ggaacctcag acttttccgg cggatctttc tccgtcatgc
    16141 ctattccaag gcggattgac atggtcccct cattgttgat gcgtacaaca ttgctggcat
    16201 aaccctttaa ccctgtatgc ctggcatcaa ggtcgaaaaa cacacacgat ggatcatgct
    16261 cagtgcctgt atttgcagcc ttaatcagga tgaacgtatc actgtttggt agtgagcgga
    16321 cgtagagacg ctttgcgcct tttccccgac atccgcagct gatcacagat ttctgcccgg
    16381 acgtgtggtg tgctttcttc agggccgact gccatccctt tacatactca tcctgtgtct
    16441 gaaattcagc cgagtatcgt ttcgtgtttg gttcgtcatc gtaagcaata aaaactgcaa
    16501 acttctggcc catgggacct cctgaaattt aaaaatacta atagcctagc catatcaata
    16561 cttcttgcgc gttgtactct tccctctcac ccaactcgga aaaaaatacg tctatgtcgc
    16621 tggctacgac gccatgtttt gcaagctctt gcagagctct ctgttttgaa atggtgaggt
    16681 cttctgctga atcaaaatac gtcatcatta cctcctgatg aaggagttga ctgaagcagg
    16741 cctttctctc cctgcccagg tttttttctg tgtaaaagtt acaatattag tgaaataaac
    16801 cattacgtta acctgcaaaa tcacacagaa gaattactat gatgccgctt caaactctcc
    16861 cttgagacga gccgcaacgg cgatctcaat acggcttcga agcaaactgg ccacggccgc
    16921 aaatacgtcg acgatgaccg aattgccgaa ctggcgatag gcctgggtat cagagactgg
    16981 aatgcgaaaa gtaacttttc ccggttgctc gaatcccata aggcgggcac attctcgcgg
    17041 ggttaaacgg cgcgggcgcc ttgccatatt tacaggatca ttaaagtcct tttcgcctat
    17101 gctgtgatcc catccacggt ctagcaaaat ttcagagcca tcacgatagt accgtgcgct
    17161 gagtgttctg caaacaactg atttatcaag tggatttacc agtccgaagc caaatccatt
    17221 accttttgcc tggtgctttt tagcgtagtt ataaaggtac tcccagagct ttggagaaag
    17281 gatgtaacgg ctatcgacct cagcgtccag cagctcccca agggttggcc gtcgtgaagg
    17341 ataaagagcc gatacaccac ttaacgaaaa gccatcatgg agcttcagat cgcgcctgaa
    17401 tccaaccaac acgatacgtt cccggtgctg aggcctgaag ttcttaccgt caattatttt
    17461 ggggtccttt gatccgtgag catcgcagtc ggcaacatcg tagccaagtt catcaagcgc
    17521 tttcatgatg atgcggaacg tatttccctt atcgtggctc ttcagatttt ttacgttctc
    17581 aagaacgaaa atggcaggct gtttggctcg gatgatccgg gcgacatcaa aaaaaagggt
    17641 cccctgggtg tcgcattcaa agccgtgttt tcggcccaaa gagttctttt tgctgacacc
    17701 ggcaatactg aacggttggc atgggaaacc tgcgagcaaa acatcatggt ctgggatgga
    17761 tgcatcgata gaagcatagg cctggtcatc cgaaacatcg ggaagattac tgagtgtaat
    17821 gtcccggata tctgagttga aatagtgctc agtctcgtca cagtaccagt ttgcactgta
    17881 tgtccggcgg gagaactgat tccattcgct tgtaaacacg catttcccgc caatggcgtc
    17941 aaaaccactc cggaggccac cgatcccagc aaacaagtca ataaaacgga aaaatttctg
    18001 gtcatagtct gctgggcgtg aggggagcaa cgatcgaaga taatgatatt cttcctgggt
    18061 tattcgacat tcattaactg aacctttgat ctggcgaata agtgatgccc tcgaccagtc
    18121 gctgccggtt gcagcattaa gttgcgcagc caaatctttt acgtcataga tacttgcaac
    18181 ctgaaccagt aatttatgga tagaggaatg tgtctcatca tttagcaatg cagttattgc
    18241 aggctcattt ctcgcgctgt acatccttac tccgtgaaat gtgattaatt tagtttctca
    18301 gactaccacg cgcagaaaat tcgatttttc gttaaagccc tttattttca tctcctgaaa
    18361 taaatttttt atccacttaa attgcctccg ttaacggaaa tttgcccggg ttctaattgg
    18421 tacaataagc acttctaatc acacgactaa tttgttacta caaaccatct aattcaggga
    18481 gaatgtatga ctctgttgaa aaagttactt ttgccgacat taattgtcct tttcccggcg
    18541 gcagttctgg ctggtcagtc ttcatttgaa gcaaaagtgg taaaaattat cgatggggac
    18601 accattaccg cgctcgacgg tcagaataca agcatcaaaa tccgtatgta cggtattgat
    18661 gcccccgaaa gtaagcaggc ctttggacaa aaggctaaac aagcgttgag ctccgcgatt
    18721 gctgcacaga atattacggt aatagaccac ggacctgata tttacggcag gatgttgggg
    18781 actatttggc ttgatggtta tgacatcaac gcttcaatgg tagacagtgg ttatgcatgg
    18841 gtatatcggt ttgacggtaa tcccatcgtc ccaaactatc ttaaattcga agcttcagct
    18901 cagaaagccg ttaaaggtct ctgggtagat cctaatcccg ttgctccttg ggagtggcgc
    18961 cagcaaaacc agaaacctca aaagacgaaa agcagggggt gagcgcgatg acaagccagc
    19021 atcctttact gaaaattgca cttaatggcg atggacttct ttcgtggcca aattcacccg
    19081 gacagactat gcgctgccca acgtgtatgc gagatgttat tacgggtccg gatggtgatt
    19141 taatacatca aatggaagag ggcgctaacc cctgcacgcc atctgttgat gttgtcattt
    19201 caaaggcgat cattgaacta ctcagcacgg gtgagcgcct ctatgtcaac ccagttaaga
    19261 acgcgaatag aacactggcc ccatcgttta tctttctgca taaagacaag caacttcgac
    19321 cattcataaa tacggaatat caaccagcag gtgccacctg gagaagtgat aaagggaaca
    19381 ggcttggttt tttctatatg gatgagcgag cgtctaaaat taataaggac cagttcgatt
    19441 ttattgctgt aatagaccca tctacttttc aagcagaatt taagacctta tggtccatgt
    19501 gtgaattcga taaccccctt gccgcgttac agcacttatt ggcttcagag aatatgtctt
    19561 cagtctgggt gaaatggcct gttaaaagcc tacaaccgag gaaagaagta gtgaaggccg
    19621 attactggta tgactattat tcacctcact acccagcgca gcaggcagct tgcacagcaa
    19681 cagtgattgg actggaagga aatgtatccg gggaaactat ttacatgctc cagatcgctg
    19741 tagaccggtc gatagttgag taccagcttg taaaatcgct aggggttata ttgttattgg
    19801 ataatacggg aaatttggta tcggagacgc accctcactt tgatataatc atcaaagctt
    19861 gcatctcggg tgtcagagac tttgtacgtc aaaatcctcg aatgcgaagt gtggagcatc
    19921 agattaccag tgcaatgcct ggttagttca atccaacaat attgagaatt actaaatggg
    19981 aaatctagat gttgtatctg cggtagattt ttacttagaa aaagcatttt atatatttgg
    20041 attcataatg cttggtatgg catggcttac ttttcgtgct ggttcaactt atcagcaatt
    20101 aaaaaatgca gataagtcgg tcagtgtagg tagaggctca ttttcaagcc aggcacatca
    20161 aataattgat gcactaagta aggataagta tatctacatt gccatcttga tattagtcat
    20221 tcacctcttg atgaagcagg taaataaaca tcacgataag cttatgactt caaggcgtat
    20281 ttatgatgac cataaatctg gcaaaattct cgatgagaca ccagctgaaa ccttgaccat
    20341 gatgaaaaaa agctttattt ccatagtagt taacttattt tcaattatta gcgtcacagt
    20401 actagctgca tgttttttca tctattttta gtcccggtaa tggtactgat agttccgctg
    20461 gtatccatgg cggtctatct gtatgagggt agagaataat tttcacgtga ca
//
LOCUS       DAMZII010000086        20327 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZII010000086 DAMZII010000000
VERSION     DAMZII010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.086"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(112..858)
                     /gene="cutC"
                     /locus_tag="POA77_003062"
     CDS             complement(112..858)
                     /gene="cutC"
                     /locus_tag="POA77_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDI6366450.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(872..1438)
                     /locus_tag="POA77_003063"
     CDS             complement(872..1438)
                     /locus_tag="POA77_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6366451.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1654..3387
                     /gene="argS"
                     /locus_tag="POA77_003064"
     CDS             1654..3387
                     /gene="argS"
                     /locus_tag="POA77_003064"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6366452.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3564..4052
                     /locus_tag="POA77_003065"
     CDS             3564..4052
                     /locus_tag="POA77_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDI6366453.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4172..4564)
                     /gene="flhe"
                     /locus_tag="POA77_003066"
     CDS             complement(4172..4564)
                     /gene="flhe"
                     /locus_tag="POA77_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDI6366454.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4564..6642)
                     /gene="flhA"
                     /locus_tag="POA77_003067"
     CDS             complement(4564..6642)
                     /gene="flhA"
                     /locus_tag="POA77_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDI6366455.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6635..7783)
                     /gene="flhB"
                     /locus_tag="POA77_003068"
     CDS             complement(6635..7783)
                     /gene="flhB"
                     /locus_tag="POA77_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDI6366456.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7985..8629)
                     /gene="cheZ"
                     /locus_tag="POA77_003069"
     CDS             complement(7985..8629)
                     /gene="cheZ"
                     /locus_tag="POA77_003069"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDI6366457.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8640..9029)
                     /gene="cheY"
                     /locus_tag="POA77_003070"
     CDS             complement(8640..9029)
                     /gene="cheY"
                     /locus_tag="POA77_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDI6366458.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9044..10093)
                     /gene="cheB"
                     /locus_tag="POA77_003071"
     CDS             complement(9044..10093)
                     /gene="cheB"
                     /locus_tag="POA77_003071"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDI6366459.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10096..10956)
                     /gene="cheR"
                     /locus_tag="POA77_003072"
     CDS             complement(10096..10956)
                     /gene="cheR"
                     /locus_tag="POA77_003072"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDI6366460.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10975..12576)
                     /gene="tap"
                     /locus_tag="POA77_003073"
     CDS             complement(10975..12576)
                     /gene="tap"
                     /locus_tag="POA77_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDI6366461.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12622..14283)
                     /gene="tar"
                     /locus_tag="POA77_003074"
     CDS             complement(12622..14283)
                     /gene="tar"
                     /locus_tag="POA77_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDI6366462.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14428..14931)
                     /gene="cheW"
                     /locus_tag="POA77_003075"
     CDS             complement(14428..14931)
                     /gene="cheW"
                     /locus_tag="POA77_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDI6366463.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14952..16916)
                     /gene="cheA"
                     /locus_tag="POA77_003076"
     CDS             complement(14952..16916)
                     /gene="cheA"
                     /locus_tag="POA77_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDI6366464.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16921..17847)
                     /gene="motB"
                     /locus_tag="POA77_003077"
     CDS             complement(16921..17847)
                     /gene="motB"
                     /locus_tag="POA77_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDI6366465.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17844..18731)
                     /gene="motA"
                     /locus_tag="POA77_003078"
     CDS             complement(17844..18731)
                     /gene="motA"
                     /locus_tag="POA77_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDI6366466.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18858..19436)
                     /gene="flhC"
                     /locus_tag="POA77_003079"
     CDS             complement(18858..19436)
                     /gene="flhC"
                     /locus_tag="POA77_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDI6366467.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19439..19789)
                     /gene="flhD"
                     /locus_tag="POA77_003080"
     CDS             complement(19439..19789)
                     /gene="flhD"
                     /locus_tag="POA77_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDI6366468.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4900 a         5578 c         4936 g         4913 t
ORIGIN      
        1 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
       61 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
      121 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
      181 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
      241 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
      301 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
      361 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
      421 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
      481 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
      541 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
      601 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
      661 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
      721 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
      781 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
      841 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
      901 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
      961 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     1021 ggctgagtcc ttcatcagaa agcaaagcca gcgcacgggc attcagcgtt tctggatcac
     1081 ccggcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     1141 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     1201 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     1261 acccgcgacg ccagcgctca gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg
     1321 cagtcagcgg agtgatatcc aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa
     1381 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     1441 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgtgc caaccaaccg
     1501 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     1561 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     1621 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     1681 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     1741 cagtcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     1801 aaactgggta tggcaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     1861 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     1921 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     1981 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     2041 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     2101 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     2161 attgcatggc tggaaaagca gcagcaggaa aacgccggtg aaatggagct ggctgacctt
     2221 gaaggtttct accgcgatgc gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt
     2281 gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat atttccgcga gatgtggcgc
     2341 aaactggtcg acatcaccat gacgcagaac cagatcacct acgatcgtct caacgtgacg
     2401 ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc caatgctgcc aggaattgtg
     2461 gcggatctca aagccaaagg tctggcagta gaaagcgaag gggcgaccgt cgtattcctt
     2521 gatgagttta aaaacaagga aggcgaaccg atgggcgtga tcattcagaa gaaagatggc
     2581 ggctatctct acaccaccac tgatatcgcc tgtgcgaaat atcgttatga aacactgcat
     2641 gccgatcgcg tgctgtatta catcgactcc cgtcagcatc aacacctgat gcaggcatgg
     2701 gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac cgctggaaca ccacatgttc
     2761 ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc gcgcgggtgg tacagtgaaa
     2821 ctggccgatc tgctggatga agccctggaa cgtgcacgcc gtctggtggc agaaaagaac
     2881 ccggatatgc cagctgacga gctggaaaaa ctggctaacg cggttggtat tggtgcggtg
     2941 aaatatgcgg atctctccaa aaaccgcacc acggactaca tcttcgactg ggacaacatg
     3001 ctggcgtttg agggtaatac cgcgccatac atgcagtatg cttacacgcg tgtattgtcc
     3061 gtgttccgta aggcagaaat taacgaagag caactggctg cagctccggt aatcatccgt
     3121 gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg
     3181 gttgcccgtg aaggcacgcc gcatgtgatg tgtgcttacc tgtacgatct ggctggtctg
     3241 ttctctggct tctacgagca ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac
     3301 agccgtctga aactggcaca actgacagcg aagacgctaa agctgggtct ggatacgctg
     3361 ggtattgaga cagtagagcg tatgtaatcg atttttcgtg agagtaaagc ctgatcaggg
     3421 ttacctgatc aggctttttt attgccatct aaatgtattc tgaaaatgga catgccattg
     3481 ttttctgact gttggataag aggccagaag cgtaatatcc ggccccaggg aaacgataac
     3541 ggttgaattt aaggaatacc gcagtgttta aatttcttgt attaacatta ggcattatct
     3601 cttgccaggc ttacgcagaa gatacggtta tagtaaacga ccatgacatt tcagccatca
     3661 aagattgttg gcaaaaaaat tcagatgatg atactgacat taacgtgatc aaatcatgcc
     3721 tgcgacaaga atacaatctc gtcgatgcgc aattaaataa agcctatggt gaagcttatc
     3781 gttatataga acaagtgcca cgcacaggtg taaaaaaacc tgataccgaa caacttaact
     3841 tgcttaaaaa atcacagcga gcctggctgg attttaggga caaagaatgt gaattaatcc
     3901 tttcaaatga ggacgttcag gatttaagtg acccttattc tgaatcagaa tggctctcat
     3961 gtatgatcat acagaccaat acgcgaactc gccagttgca gctataccat aactctgaag
     4021 atttttatcc aagccctttg acaagaggat aattcacatc tttttggcat gttttgttgc
     4081 aagctattcc tgataaataa ttgcaacaag acatcgagcc tttttcactg agttattaaa
     4141 catactcgcg agcgcgtaat ttttttgtcc ttcagcgata attcacaatc acttcattac
     4201 gttgtacctt tagcggtgga attaaccgcc caccgcctgg cacttcccag ataaatcgca
     4261 acggttctgc tgccgctata ccggaaaagg ccacggtggt tccgctctgc ccctctaatt
     4321 cgacacaacg agattgcgaa cacaagcgaa cccgcagtcc tgaaggtgtc gggccgataa
     4381 gctgataacg ccacgctacc agcgtcatca atcctgaagc aggttgtcgc gtagaaagag
     4441 gcgcagacga catcgactca ccgcgatgat ttagcgtaat tcctacacta cttgcctgcc
     4501 acatcccctc cccggcggct tgcaccagca gcggaaacaa taatattgct aataaggttc
     4561 tcattatttg ccgccaattg tcgccgtcat gcggatatgt cggttatcag acagttccag
     4621 attcgacagg accactaact gcggcaagct gcggcgcagg aagcgagaca ataatggtcg
     4681 cagcgcgtgg ttcaccaaca atactggcgg cgcacccagc atctcctgac gggatagcgc
     4741 ttcctgagtt tgcgccagta aacgatccgc cagccctggc tccagtcctc ccccgccctg
     4801 cagcgcctgt agtaacaaac gttccagcgg tgtatcgagg ccaataacat ggacttcatc
     4861 tttgccagga aaccactgct gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt
     4921 taattcatgt ggatcgcttt ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg
     4981 catatcgcga atcggtactt tttcatcgag gagattttgc agcactttat gcagtgtggt
     5041 gagcgtgacg acgccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg
     5101 atccaacagc tgttgcgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag
     5161 gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg
     5221 cgcctgttct tttagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgac
     5281 ggtcgcctca ccaggtaacg tcccggcagc ggttccaggg ttaatcgcca gccagcgccc
     5341 cggataagca tcaccactgc caatctccac gcctttcatc aaaatgcgat agcgggcagg
     5401 ttgcagatcc atattgtcgc gaatgtgcac cactggcggc agaaatccca tctcctgggc
     5461 aaatttcttg cggatactgc gtatacggcc caacaactca ccatcctgct ggaaatcgac
     5521 catcgggatc agtcgataac ccacttccat tcccagagaa tcttccagtt gtacatcgtt
     5581 ccacgtcgct tcgacaacgg tattattctc tgccattttt accggtttgg gttcggcagg
     5641 cgctttttgt tcgcgtccgc gtatccacca ggccagcccc agcaatccgg cagtgaacag
     5701 caaaaatacc aggttcggca ttccaggcac caggccgagt aaaccgagca cggcggcgct
     5761 taacaacata acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg
     5821 atcggtgctg acacgcgtaa cgatgacccc cgcggcggta gaaatcacca gcgccggaat
     5881 ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa ctttccgccg cgtgtcccat
     5941 gctcatgcca tgttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat
     6001 gaggatcccg gcgatggcat cgccgcgaac aaacttactt gccccgtcca ttgagccgta
     6061 aaaatcggct tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa
     6121 tccagcgtta aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa
     6181 gcgcgcaccc acttctgcga tacgcccggc acctttggta atgaccataa agttgatgat
     6241 cacgagaatg acaaacacta cgataccgat agcgaaattg ccaccaacga ggaagtgacc
     6301 gaatgcttcg accaccttcc cagccgccgc cgcgccggta tgcccttcca tcaaaatgat
     6361 acgggttgaa gccacgttaa gtgccagacg caacaacgta gtaaacaaca gaatggtcgg
     6421 aaacgcagca aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga
     6481 caaggcaata ttgaaggtaa acaacaggtc gagtatgaat gcgggcagtg gcagcaccat
     6541 catcgacaag atcaacagga tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt
     6601 caggtttgcg ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa
     6661 taaaatccag ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag
     6721 ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca
     6781 gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg
     6841 gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca
     6901 gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg
     6961 ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca
     7021 tccgacgccg tgcagcagct cgctgcatct gacggatccg ccctttaaca tgagggtcac
     7081 cttcgctttg tttgaactca tcacgaatat cctgccgtga catacgcagc tttttcaggt
     7141 ggctgaatat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
     7201 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggagactcgg
     7261 ccatcaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
     7321 ccaggatggt tttcaaaatt gctttaagca actccgcgcc agtctgagcc gagaacatcc
     7381 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa
     7441 ataccagccc tcccagcatg accggagaaa tgagcgccac cagcaccacg ccgctaatca
     7501 gcggcagcag cgccagcatg gcttctctga tcagcagaat aatctgcccg aggatcagat
     7561 tcgggtcatt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
     7621 gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca
     7681 gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt
     7741 gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa
     7801 gagtattaag cgtgaatgat gccagagcgc aaagcgttca atggtttgag taaggggcaa
     7861 aacaggcggg atttagggct tttgctgcca cacatcaagc atagtgtgcg tttgtcggat
     7921 gcggcgtcat cgccttatca gaccgcctga tatgacgtgg tcacgccaca tcaggcaata
     7981 caaatcaaaa tccaagacta tccaacaaat cgtccacctg atcctgactg gctaccacgc
     8041 cggctttgct ggtatcgacc tgaggtccat taagcaaact ctggttttca cgttttggac
     8101 gcgactcctg ttccgggatg ttttccaaca gcaccatcag caactggcgt tcgatctcct
     8161 gaatgacatc catcatccgc ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca
     8221 tcatgatttc cagcagttgc gcgttagtaa agctggtatg cgcgggtaca tctgccagaa
     8281 attgtcgtgt atctgttacc agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc
     8341 agtcatccca acgttgggtt aacgcttttg ctgatttctc catttgatcc tgatgcggtt
     8401 gtgacgcctc aacactgttc agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat
     8461 agtacaaacg atcgcgcgca tcggggatgg cttccgccgc ttcggcaatg gcctgatcca
     8521 gccccagttc ccgcaaactg tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga
     8581 tatcgccagc tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct
     8641 cacatgccca gtttctcaaa gattttgttg agtttttcct ccagcgtcgc ggcggtaaat
     8701 ggcttcacca catagccact ggcccccgct tgcgccgcag caatgatgtt ctctttcttc
     8761 gcttctgcag tcaccattaa cactggcaat gccgacatcg cgccatccgc acgaattgtt
     8821 ttcagcaatt ccaggccatc catattgggc atgttccagt cggagataac aaatccataa
     8881 ccgcctgcct gcaacttatt gagagcgtcg acgccatctt ccgcttcctc aacattattg
     8941 aatcccagct ctttaagcag gttacgcact atgcgtcgca tggtggaaaa gtcatccaca
     9001 accaaaaatt taagttcttt atccgccatt tcacactcct gatttaaata cgtatcgcct
     9061 gtccggcact aatttttgcc aacatttgct ggcttacctg gctaagatcg accacttcgc
     9121 agacaccacc catattgatg gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt
     9181 tttgcgcaag ggtccatgcc cccgcctgac gcatcgccaa cattcccgcc gcgccgtcgt
     9241 tgcccatacc ggtcaggatc accccaaccg cattacgccc cgcctgtttg gcgacagaat
     9301 ggaacaacac atctaccgaa ggccgatgac ggttaaccgc cgggccatcg tgaattttga
     9361 tttggtaatt tgcgccacta cgcgacagct ccatatgccg atcgcccggc gcaatatagg
     9421 catgccccgg caagacacgt tctccgtctt cggcttcttt aaccccgatc tggcaaagct
     9481 tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg catatgttgg gtaattaaca
     9541 atgccgggct ggaaagcggc aacggttgca gtacgtgacg aattgcctca gttccacccg
     9601 ttgaagcacc aatcgcaatc agtttttcag aactcaacaa cggacccgcc ttcagcgttg
     9661 tcggtgctga caatggctta tgtgctgcaa ggctcgcctt tgctgccgta cgcacctttt
     9721 cagcaatcat ttcgctatac gccagcattc cttcgcgaat acccagttgc ggtttggtga
     9781 caaaatctat tgcccccagc tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca
     9841 aggaagaaac catcacaacg ggcattggac gcaaacgcat taatttttcg aggaaatcca
     9901 gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac atcgggattg aatttcttaa
     9961 tcaagtcacg cgcgaccagc ggatcaggcg cggtcgccac catttccatg tcgctatggc
    10021 tgttgatgat ttctgtcatg atctggcgca tcagtgccga atcatcgaca gataacaccc
    10081 tgattttgct catcgttaat ccttacttag cgcatacacc gtctgaccac gcagcgtgaa
    10141 gcggcgctca aggtggctaa agttttcaga gtgacccgca aacagcaatc cgtcgggttt
    10201 aaggagcgga acaaagcggc gcaaaatctc ctgctgggta ttttgatcga agtagatcat
    10261 gacgttacga cagaagatcg catcaaacgg ccccggcacg gtgtactgtt tctccagtag
    10321 attcagcggg gcaaaatcaa catagttcgc cagctcctga cgcacgcgta ccagcccttc
    10381 atgcggtccc gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa
    10441 ctcttcatgg cgataaatac cgcttctggc tttttccagc acttcggtgt cgatatcact
    10501 ggcaaacact ttccagcgtc cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat
    10561 gctgtacggc tcttcgccgg tcgaagccgc cgcgctccat acgcgatact cgccagaacg
    10621 gcgacgtgcg tgatccgcga gcagagggaa atggtgtgcc tcacggaaaa atgccgtcag
    10681 attcgtggtc agcgaattga taaacgcctg ccactcaccg ctgtgctgat tagattccag
    10741 caagttcaga taatgaccga aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg
    10801 gttgtaaacc atgtcgcgtt tatggtcagc cagaacgatc ccggctcgtt gatagatcaa
    10861 ttgacttatc cgccgaaaat gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa
    10921 taaagacgtt tgcccacagg gcagagatga agtcatagcg ccttctcaat cacttcagga
    10981 taccactggc gcaatttgta actgcgccga ctcatgtcgt gctacttcat gttcttcaag
    11041 ggtaaatacc gccacacgcg acgaaagatg gtcggcctga ttcgccagtt gttccgttgc
    11101 caccgccgct tcttctacca gcgaggcgtt ctgctgagtc acctgatcca tctggctgac
    11161 agcctgtgca acctgctcaa tcccccgctg ttgttcttcc gacgcagagg caatttctcc
    11221 cataatgtcg ttcacgcggg tcaccgaact gacaatatcg atcatggtcg cggcggcgtt
    11281 attcaccagt ttcgaaccct gctggacacg attgactgac tcttcgatca gccctttgat
    11341 ctcttttgct gcctgcgcgc tacggctggc aagattgcgc acttcacctg ccactaccgc
    11401 aaaaccacgc ccctgctctc cggcgcgagc cgcttccact gccgcattca gggccagaat
    11461 attggtctgg aaagcaattc cgtcgataac gctgataatg tcgccaattt tttgcgagct
    11521 ggtggcgatc tcctgcatgg tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc
    11581 cgttgtcgcg gcattttttg ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc
    11641 taccgtggcg gttaattgct ccatactggc ggccgtttgt gccagcgatg ccgcctgctg
    11701 ttcggtacga cttgagagat cgttattgcc tgcgacaatc tccgcgatac caatgtgcat
    11761 ctcctggctt cccttacgca catcacttac cgtcccacgc aaagcctgct gcatggtctt
    11821 cagactggca aaaatggcgg tgatctcatt acgaccatat accgcaatcg gacgcgccag
    11881 attacccgca gcaatgctgt caaaatggct accgataatg gccagtggtt gaacaatcat
    11941 cttgcgcgtc caccacagcg cactgctgat gtagattgcc gcaacaataa tcatgctgat
    12001 aaacaccagt gccgaaatct gatagttacg ctggctttgc gcactggcgg cttccagcac
    12061 atggttgatc tccagctgcc aggcctcaaa gttaacgtca aacgcattct gtgatccctg
    12121 caccggcgca gtgaggaaat ccgaaagttg attactttca agccaggtcg cctgatgttc
    12181 gagatcgttg tgccagcggg caaaactttt ctccgtctct ttttgcaatg ccctgacgtg
    12241 ctcgttgccc gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact
    12301 ggcgcgcgcc gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gtgccgtcag
    12361 agtgcccgct ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa
    12421 tgccgcacgt tgctgattgc tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa
    12481 tgcccaaaaa gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa
    12541 aaacagcgtg gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat
    12601 gaccgcgtta gtaaatactc gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg
    12661 cagtcgtggc tgagccggtg gttgctcact ggcaggacgg gatggtgttt gcggtttatt
    12721 ggtgagtggg ctggctgcca gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg
    12781 ttcttccagc gcagcggcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt
    12841 gacgcgatcc atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc
    12901 cgatgccgat gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt
    12961 gttcattgtt tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gtgagacgga
    13021 gtcttcaatg agggctttga tctcttttgc cgcctgggcg ctgcgactgg caagattacg
    13081 cacttcaccc gccaccacgg caaaaccacg gccctgttca cccgcacgcg cggcttcaac
    13141 cgcggcattc agcgcgagga tattagtctg gaaggcaata ccgtcgataa cgctgataat
    13201 gtcggcaatt ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac
    13261 cactttgccg ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg
    13321 ggcgttatcg gcgttttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc
    13381 ttccagcgcg gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat
    13441 ttcacgggta ccagcataga tggcatctga accttcgcgg acatgagtga cggtgtcagt
    13501 caaagagcgt tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc
    13561 aatggtcagg gtattcgcca ggttaccacc ggcgatttcg cgaatgtgag caataatttt
    13621 tgccagcgga gtaagcaaca tacggcgaat gccgtaccac gccaccagca gaatcaatac
    13681 caccaccagc gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc
    13741 agtgacgata tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc
    13801 cattgcattt tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc
    13861 tagataatca atcagttcag ttaacgctgt gtaatagttt ttatattttt catcaatatt
    13921 acgactggta gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgcgt
    13981 cgctgcctgc gccaatgttt tcctggcgct atcgagcaat tcaactttgg cgttactttg
    14041 ttgattagag gaatccatca tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg
    14101 cagcattaaa tcccaggttg acgtcagctc gccctgctgt tcccgcaatt gattggaaac
    14161 cacaaagctc ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg
    14221 taacagtgcg aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat
    14281 cataaggcac cttcctgaaa acaagttgat ctcgttatcg gcaagaaggg gggaaacttt
    14341 attgctgatg ccacccgccg cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt
    14401 tcatttcaat tgaggaaatc gggagaatta cgccacttct gacgccgcgc tatctaacag
    14461 cgccatctct tcgctgttca gcagtttttc gatgttcacc agaatcaaca tacggtcgcc
    14521 cagtgcgccc agtccagtga gatattctgt tgaaagcgtc acggcaaact ctggtgccgg
    14581 acgaatttgc tccgccgtca atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac
    14641 cacccgctgt ccgagattca ggacgataac taccgtgttg tcgttatagt ccacatccac
    14701 ctggctgaac ttaattcgta agtcaacaat cggcacaata acgccgcgca gattcgtgac
    14761 gcctttgata aacgctggcg tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc
    14821 ctgcactttc aggatatcaa taccgtactc ttcatcacca agggtaaata ccagaaattc
    14881 ctggcctgac ggctcgctgg ccagctttgt tacattcgtc ataccggtca tattgttacc
    14941 tttttactca ttcaggcggc ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag
    15001 gcggagacat caacaatcag tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg
    15061 ccggggactt tgcgatagtt actttcaagg tttttaacca caacctggtg ttgaccaatt
    15121 aattgatcca ccagcaaggc gtagcggcga ccgccacttt gtaagatcac aacaattccc
    15181 tgggtggctt cggttttcgc gcccgcgacg ttgaacactt tccacagttc gacgatgggc
    15241 agatattcac cccgcacttc cagcacccgc tcgccgccgg ccagtggatg cagatcggct
    15301 tcacggggtt gcagtgattc catgacagca ttcagcggca gaatgaaaac ttcatccgca
    15361 acgcgtacgg acatgccgtc gaggatggcc aacgtcagcg gcagtaaaat gcggatcgta
    15421 gtgccagtac cctgcttcga ctggatttcg acatgaccgc ccatctcctg gatattacgt
    15481 ttaacgacgt ccatgccgac gccgcgcccg gagacgtcgg tgacctgctc cgccgtggag
    15541 aagccaggtg caaatatcag cattgcaact tcgtcgtcgc tcatgttttc gctgacagtc
    15601 aaaccttgcg aggccgcttt tgccagaatt cgctcacggt ttagccccgc cccatcgtcg
    15661 gtcacttcaa tgcaaatgtt gccgccctga tgttcggcag acagaattaa atttccgacg
    15721 ctgtttttac ctgcggcgag ccgtttttct ggcagttcaa taccgtgatc gaggctattg
    15781 cgtaccaggt gggtcagcgg gtcgataatg cgttctatca ggcttttgtc gagttcagta
    15841 gaactgccca ccagcgtcag ttctacctgc ttgccgagtt ttcccgccag atcacgcacc
    15901 agccggggat agcgactaaa aacatattcc atcggcatca tgcgaatcga catcactgat
    15961 tcctgcaaat cacgggcgtt acgttgtaac tgccccatgc tggttatcaa atcaccatga
    16021 ttaaccgggt ccagttcgct ggaacgctgg gcaagcatgg actgggtgat aaccagctcg
    16081 ccgacgaggt taattaattg atcaaccttt tctaccgcta cacggatgct ggtggattca
    16141 ttgctgcgcg tcgttttttc ccgctccacg cggccggttg gcgcttgttc ggctgccagt
    16201 ttaagcactg gtggggtgga tatttttggc gagacttcta ctgtttcaaa agtaatctga
    16261 tcggcttcaa tcacaaaaca gagtaccgct gtgatgtcat cttcggcaat gtcgcccggt
    16321 aatattgccg agagcgaatc cgcccctttc accacgtcag ttaacgttgt cagatgtccc
    16381 agttcttctt ccagcaggtc gacttccccg gccttcaggc gcgaaaggat aattcgtcgc
    16441 ggcgactgac tgcgactctg ctcatcttgc ggttcacttt tggcaaccac acttaatcgg
    16501 gtcactgcgg atggcgtttc gcctttcgct tctaatgcca gttgacgcaa ggcctggcag
    16561 atataatcga agctggcggc atccggctct tgcgactgtt tataagcgtc gagctgttct
    16621 tgcatgatgt ccttcgtttc caaaaacaga ttgataatgt cggtgttgag ttgcatctca
    16681 cctcgtctgg cttcatcgag caggttttcc atcagatgcg tggtttcctg caaaacgctg
    16741 aagccaaaag ttcctgcccc tcctttgatc gagtgggcag cccgaaagat ggcattcaat
    16801 tgttcggcat ctggcgcttc cggctgcaaa accagcagat gctgctccat gtcagccaac
    16861 agttcgtccg cttcatcaaa aaatgtctga taaaaatcgc ttatatccat gctcacgctg
    16921 tcacctcggt tcggctgatg gcattgtggg aacactgacc tgtggtgcaa cctcaggttt
    16981 ttccagggcg cttactggct cattctggct ttcggcgttt tcatgcaaaa tggcctgttc
    17041 ggcttgtttg ttcagtacca gcaggctgat gcgacggttg acggcatcat caggtccgcg
    17101 atcgcttaag cgcatcgttg ccgccatgcc gacaacacgt aacactttgc cgctatccaa
    17161 cccgccgacc atcagttcgc ggcgggatgc attggcccga tcggcagaaa gctcccagtt
    17221 gctgtaacct ttctcaccgc tggcgtaggg aaaatcatcg gtatgacctg aaaggctaat
    17281 acggttggga ataccgttca gtacaggcgc gatggcgcgc agaatgtcgc gcatataggg
    17341 ttcgacatcg gcactgccgg ttctaaacat cgggcgattc tggctatcga tgatctgaat
    17401 acgtagacct tcctggacca gatcgatttt gagatgtgga cgcaacgccc gcagtttcgg
    17461 atcggactct atcaactggt cgagatcacc ccgcaatttc cgcaatcgac tttgctccat
    17521 gcgttttttc aactcttcga tgttcggctg cttattcact tccccctggc tttgggtgta
    17581 atcatcacca ccgccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac
    17641 cgcagtcgcc agtggagtcc ggaagtactc cgcaatctga atcagctctt ttgggctgga
    17701 gatggagatc agccacatca ccagaaaaaa ggccatcatc gcagtcataa agtcggcata
    17761 agcaatcttc cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac
    17821 aataatcgga tgcgcttgat tcttcatgct tcctcggttg tcgtctgttg ttgcggattt
    17881 ttcaccgcac gcacatgctc ttccagttca ataaacgacg gacgttcgct ggaatagagc
    17941 gttttgcgac caaactcaac ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga
    18001 gtgactttga cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacactc
    18061 gctaatgggg aaataaatcc gtaagccaat aaaatgccga ggaaagtccc caccatcgca
    18121 tgtgcgataa gcgcacccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg
    18181 acccccatta cagccgcgac aataccaaac gccggaagtg agtccccgac cagcgccaga
    18241 ctgttcgccg ggacttctgc ctcgctttcg tgcgtctcaa tctcttcatc catcagggct
    18301 tcgatttcga aggtgttcat atgaccgctg ataatcagac gcagataatc gacgataaaa
    18361 tcaagcatga cgctatccgc gaggatgcgt gggtagctgg cgaagatctc gctctcacgg
    18421 ggattttcaa tatcacgttc cagcgaaaac atccccatct gccgcgattt cgccatcaac
    18481 cgataaagca gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac
    18541 agcaacggca gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac
    18601 gacccaatcc ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg
    18661 cttccaccgg tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat
    18721 aagataagca cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc
    18781 gttaacgcgc gattggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt
    18841 gcgcctcacc gtatcagtta aacagcctgt actctctgtt catccagcag ttgtgggata
    18901 atatcggcag gattctggga aagtttacgt ctttttactg cccgggatgg cggttgacat
    18961 aagctgcagg caaagctgcc aacaggctgg tgagcgtggg taataaaatt gccgccgcag
    19021 cagttgcagc tggaaagttg cagtaatcca ctttcaacaa accgcaccaa tgtccaggca
    19081 cgggttaatg ccagcagtgg tccttcttct gcttgtgggc actgttcaag gtataaacgg
    19141 taggctttga tcaccgcatc gacgccatta cacaaaccgg ttttcagtaa aaactgccat
    19201 gcattacaga acatcgaagc atgaacgttt tgttcccagg tcataaacca gtcggttgag
    19261 aatggcagca tgcctttcgg cggtgggctt ccgcgcagtt ctttataaag ttttatcagg
    19321 cgtccgcgac ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc
    19381 aattccattg ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc
    19441 aggccctttt cttgcgcagc gcttcttcag gctgattaac atcattcagc aatcgtgttg
    19501 agagcatgat gccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact
    19561 gagtaatcgt ctggtggctg tcaaaacgga agtgacaaac cagttgattg gtttctgcca
    19621 gcttaaccat ttgcggaaga gtcagtgccg ctaacgttgt cgccatttct tcatttatgc
    19681 cgagacgaaa catagcggac gctttgtcct gaacaatcaa acgctgtgca agtagtaaat
    19741 atgacaagtt gatgtcataa atgtgtttca gcaactcgga ggtatgcatt attcccaccc
    19801 agaataacca actttatttt tatgcggttt caccgcaccc cgtgatgtcg ccgggaagcc
    19861 ccggtaaaaa ataattagca ttagaatagt tgcgataagc tgcaataagc agaaccacct
    19921 ttttggttta atatgtcctt acaaatagaa atgggtcttt acacttatct aagatttttc
    19981 ctaaatcgac gcaactgtac tcgtcactac acgcacatac aacggagggg ggctgcgatt
    20041 ttcaataatg tgtgatgcag atcacacaaa acactcaatt acttaacata aatgtataag
    20101 tcatactttt gttttgggtg tatttcaatc tgttaaaaag tttttcgcta cgctagcaag
    20161 caaaaatgaa acaggaataa tcgaaatggg atgttgcgca cagttaaaat aactcaccgt
    20221 aaataatcat ctgctataaa taatcacttt catgcaatac cagataagct atttttaaac
    20281 agacacttac cgcacaacaa actaattaaa tacaaagaaa ggcaaca
//
LOCUS       DAMZII010000087        20252 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZII010000087 DAMZII010000000
VERSION     DAMZII010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.087"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            27..1004
                     /locus_tag="POA77_003081"
     CDS             27..1004
                     /locus_tag="POA77_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDI6366469.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1074..1556)
                     /locus_tag="POA77_003082"
     CDS             complement(1074..1556)
                     /locus_tag="POA77_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDI6366470.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1791..4373
                     /gene="leuS"
                     /locus_tag="POA77_003083"
     CDS             1791..4373
                     /gene="leuS"
                     /locus_tag="POA77_003083"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI6366471.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4388..4969
                     /gene="lptE"
                     /locus_tag="POA77_003084"
     CDS             4388..4969
                     /gene="lptE"
                     /locus_tag="POA77_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDI6366472.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4969..6000
                     /gene="holA"
                     /locus_tag="POA77_003085"
     CDS             4969..6000
                     /gene="holA"
                     /locus_tag="POA77_003085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI6366473.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6002..6643
                     /gene="nadD"
                     /locus_tag="POA77_003086"
     CDS             6002..6643
                     /gene="nadD"
                     /locus_tag="POA77_003086"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDI6366474.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6667..7278
                     /locus_tag="POA77_003087"
     CDS             6667..7278
                     /locus_tag="POA77_003087"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDI6366475.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7538..7855
                     /gene="rsfS"
                     /locus_tag="POA77_003088"
                     /gene_synonym="rsfA"
     CDS             7538..7855
                     /gene="rsfS"
                     /locus_tag="POA77_003088"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI6366476.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7859..8326
                     /gene="rlmH"
                     /locus_tag="POA77_003089"
     CDS             7859..8326
                     /gene="rlmH"
                     /locus_tag="POA77_003089"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI6366477.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8357..10258
                     /gene="mrdA"
                     /locus_tag="POA77_003090"
     CDS             8357..10258
                     /gene="mrdA"
                     /locus_tag="POA77_003090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDI6366478.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10261..11373
                     /gene="mrdB"
                     /locus_tag="POA77_003091"
                     /gene_synonym="rodA"
     CDS             10261..11373
                     /gene="mrdB"
                     /locus_tag="POA77_003091"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDI6366479.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11384..12472
                     /gene="rlpA"
                     /locus_tag="POA77_003092"
     CDS             11384..12472
                     /gene="rlpA"
                     /locus_tag="POA77_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDI6366480.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12611..13822
                     /gene="dacA"
                     /locus_tag="POA77_003093"
     CDS             12611..13822
                     /gene="dacA"
                     /locus_tag="POA77_003093"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDI6366481.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13933..14196
                     /locus_tag="POA77_003094"
     CDS             13933..14196
                     /locus_tag="POA77_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDI6366482.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14297..14938
                     /gene="lipB"
                     /locus_tag="POA77_003095"
     CDS             14297..14938
                     /gene="lipB"
                     /locus_tag="POA77_003095"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDI6366483.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15197..16150
                     /locus_tag="POA77_003096"
     CDS             15197..16150
                     /locus_tag="POA77_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDI6366484.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16359..17324
                     /gene="lipA"
                     /locus_tag="POA77_003097"
     CDS             16359..17324
                     /gene="lipA"
                     /locus_tag="POA77_003097"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI6366485.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17425..17628)
                     /gene="tatE"
                     /locus_tag="POA77_003098"
     CDS             complement(17425..17628)
                     /gene="tatE"
                     /locus_tag="POA77_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDI6366486.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17757..18545)
                     /locus_tag="POA77_003099"
     CDS             complement(17757..18545)
                     /locus_tag="POA77_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDI6366487.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18638..19021
                     /gene="crcB"
                     /locus_tag="POA77_003100"
     CDS             18638..19021
                     /gene="crcB"
                     /locus_tag="POA77_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6366488.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19076..19285)
                     /gene="cspE"
                     /locus_tag="POA77_003101"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19076..19285)
                     /gene="cspE"
                     /locus_tag="POA77_003101"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI6366489.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19460..20020)
                     /gene="pagP"
                     /locus_tag="POA77_003102"
     CDS             complement(19460..20020)
                     /gene="pagP"
                     /locus_tag="POA77_003102"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDI6366490.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5109 a         5110 c         5090 g         4943 t
ORIGIN      
        1 ttatctgcaa ccataaggaa atgataatga ttttcacgtc aacttgctgc gataatttat
       61 caatagatga aattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
      121 ggttttatta taatcgcgat agcgcaattg attctccgga cgacgaaaaa gccttttact
      181 ggctgaagct ggccgccgag caaggtcatt gtgaagcaca atattcctta gggcaaaagt
      241 atactgagga taaaagccgt cataaagata atgagcatgc catcttttgg ctgaaaaaag
      301 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggatactg gatcgtggag
      361 aagaccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
      421 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
      481 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
      541 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacgag acactcgccg
      601 cattctggta tttgaaaagc gcacagcagg gtaatcggca tgctcagttt caaattgcat
      661 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
      721 tgaaggctgc cgctcagggg agcgtcggcg cttacgtcaa catcggttat atgtataaac
      781 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
      841 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
      901 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
      961 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
     1021 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
     1081 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
     1141 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
     1201 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
     1261 agctgcgttt tatcggtgat gtctgccagc tcctgccaca agctttcttt aatcacccgc
     1321 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
     1381 aggtcacgtc tgacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
     1441 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
     1501 aggcgttcgc tcagtgacgc aaccagttca cggtaatatt gagcaacctt gttcatcgtt
     1561 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
     1621 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
     1681 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
     1741 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
     1801 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
     1861 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
     1921 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
     1981 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
     2041 tgcctgcgga aggcgcggcg gtgaaaaaca ataccgctcc ggcaccgtgg acgtacgaca
     2101 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
     2161 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
     2221 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
     2281 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
     2341 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
     2401 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
     2461 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacacgctga
     2521 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
     2581 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
     2641 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
     2701 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
     2761 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
     2821 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
     2881 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
     2941 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
     3001 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
     3061 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
     3121 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
     3181 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
     3241 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
     3301 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
     3361 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
     3421 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
     3481 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
     3541 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
     3601 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
     3661 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
     3721 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
     3781 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
     3841 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
     3901 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
     3961 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
     4021 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
     4081 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
     4141 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
     4201 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac
     4261 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
     4321 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
     4381 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
     4441 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
     4501 ggactcaggc gatccgaacg ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa
     4561 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
     4621 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
     4681 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
     4741 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
     4801 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
     4861 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
     4921 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
     4981 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
     5041 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
     5101 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
     5161 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
     5221 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
     5281 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
     5341 gcctggttta ctgcgcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
     5401 gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac
     5461 gctgcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
     5521 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
     5581 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
     5641 aaaagtaagc gcgcgttgca tattcttcag caactgcgtc tggaaggcag cgagccggtt
     5701 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
     5761 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
     5821 atgatgggcg aggcgttaaa tcgcttaagc cagccgcagt tacgtcaggc cgtgcaactc
     5881 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
     5941 gaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
     6001 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
     6061 taaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
     6121 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
     6181 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
     6241 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag ggccggacgt
     6301 gccgctggcg tttattattg gtcaggattc attgctgacc tttccgacct ggtacgaata
     6361 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
     6421 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
     6481 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
     6541 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
     6601 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
     6661 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtcta
     6721 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
     6781 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
     6841 cagcataccg cgcgactggt tctcagtgac cgccagctcc ccgttcatat catccctgaa
     6901 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
     6961 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
     7021 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
     7081 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
     7141 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacca ggggtgttgg
     7201 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
     7261 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
     7321 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
     7381 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
     7441 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
     7501 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
     7561 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
     7621 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
     7681 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
     7741 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
     7801 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
     7861 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
     7921 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
     7981 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
     8041 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
     8101 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
     8161 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
     8221 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
     8281 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
     8341 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
     8401 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
     8461 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
     8521 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
     8581 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
     8641 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
     8701 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
     8761 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
     8821 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
     8881 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
     8941 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
     9001 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
     9061 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
     9121 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
     9181 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
     9241 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg acccgaatac
     9301 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
     9361 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
     9421 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
     9481 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
     9541 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
     9601 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
     9661 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
     9721 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
     9781 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
     9841 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
     9901 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
     9961 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    10021 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    10081 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    10141 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    10201 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    10261 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    10321 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    10381 caggatattg gcatgatgga gcgcaaaatc ggccaaatcg cgatgggtct ggtcatcatg
    10441 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    10501 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    10561 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    10621 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    10681 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    10741 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    10801 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    10861 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac
    10921 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    10981 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    11041 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    11101 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    11161 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    11221 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    11281 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    11341 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    11401 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    11461 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    11521 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    11581 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    11641 tgatgccgaa ccaggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    11701 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    11761 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    11821 ttcgctttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    11881 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    11941 agtcgccaaa cagacttacg ccctgcctgc acctcccgat ttaagcggtg gcgcgggggc
    12001 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaactcga cgctaaaaag
    12061 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    12121 agcgcctggt gtactggaaa gcagcgaacc gacacctgca ccacagcccg ttgttacagc
    12181 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    12241 cgccagcggc aactttatgg tgcaagtcgg ggccgttagc gatcaggctc gtgcgcaaca
    12301 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    12361 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    12421 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    12481 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    12541 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    12601 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    12661 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    12721 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    12781 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    12841 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    12901 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    12961 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    13021 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    13081 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    13141 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    13201 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    13261 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    13321 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    13381 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    13441 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    13501 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    13561 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    13621 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    13681 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    13741 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    13801 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    13861 actaagcatc agtaaaaaaa ctcccgcctt ctggcgggag ttgctattta attacgttac
    13921 gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac tccttttact
    13981 tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga agtggtacag
    14041 cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg caactaccac
    14101 tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact gtatgaagaa
    14161 ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt acccggctcc
    14221 tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt tactcattct
    14281 ccacggagat gccgttttgt atcaggataa aattcttgtc cgccagctcg gtcttcagcc
    14341 ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg atagtaccct
    14401 tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg caggaaaagc
    14461 ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt
    14521 gacttatcac gggccgggac aacaggtgat gtatgtgttg cttaacctga aacgccgtaa
    14581 actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata ccctggctga
    14641 actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa
    14701 aatttgctca ctgggtttac gaattcgacg cggttgttca ttccacggtc tggcattaaa
    14761 cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg ccggaatgga
    14821 aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg ctccacgttt
    14881 actggaaaat attttagcgc tactaaacaa tccggacttc gaatatatta ccgcttaatt
    14941 ccatacttca atggcccaat ttacactggg tcattactca taatacctgc taatgcttta
    15001 catctccttc gcgtttgtat attttcttta tcacttgcag aaatgcggtg ccacgccttg
    15061 ctccgtaact cactccattg attaacctat tgaaatagtt gattatggtg agcaaaagcg
    15121 ttccgttcca attagacata agattattgt aattttttaa atattatttt tccatgaata
    15181 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    15241 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    15301 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    15361 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    15421 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    15481 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    15541 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    15601 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    15661 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    15721 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    15781 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    15841 acttctgtta ccggaagagc aaaatttcag cggtctgcgg cagagagttc aagaaatgtt
    15901 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    15961 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gccgctgctg
    16021 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    16081 ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga gtaatagatg ttatccgtaa
    16141 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    16201 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    16261 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    16321 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    16381 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    16441 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    16501 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    16561 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    16621 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    16681 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    16741 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    16801 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    16861 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    16921 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    16981 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    17041 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    17101 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    17161 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    17221 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    17281 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    17341 tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt ttttgtatcg aacaagatat
    17401 tgagggagcg tcctgctcgc cacgtcactc tttatgagag agcttttcag cctgaagatc
    17461 aacgtctgcg ccttttttcg ccgcagcatc atcatcattc atcgccttct tgaacccttt
    17521 aatggccgct ccaaggtctc cgcccagcgt acgtaacttc ttagtcccaa acagcagaac
    17581 gaccagcgcc gcaactacca gcagtttggt aatactaatc tcacccatag ataccttctt
    17641 gacatataac cggcaacaga acgcgcctta aaaaccagag aatattaacg gtcattcgac
    17701 gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat
    17761 aataattgcg gcggcgcaaa gcgacggttg tttaagacgg gcagttgcgc gcgcacctga
    17821 cgcacacgtt cgggcgtcac ttccgccata atgagtgcag gcatttctga cgctgccgca
    17881 atggtgacgc caaagggatc tataatccgg ctttgaccga tatttttgtt cccgcactcc
    17941 cccgccgcca ccatataaca ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag
    18001 tgatgctctt tgagcggccc gcgaacccag gcggcaggaa gtaccaggat ttcagctccc
    18061 tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt cataacaggt catcagaccg
    18121 accttcatcc cctccacctc gagtaacgga gcgatttcat taccagcatc aacacggcgt
    18181 gattcctgaa tggcaaatgc atcatagaga tgcaatttgg cataacgggc gacgatgttt
    18241 cctgcctgta gcgccaccag catattccat gcgcgccccg gcgttgaagg aacatgaatc
    18301 gtcagaattg tcgtcatcat gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg
    18361 ccttccagca gctgtgctga tttaaccgat agatctgcat catgatcatc gcgcgccagc
    18421 aatgcttccg gcaggacgaa cagcgatacg tcgttttccg ccgcctgcgc catcaacgag
    18481 gcacaaatct cagcgttctt ttcccacaca gatgtaacag caaactgtcc tgcggcaacc
    18541 agcattgcca cctcctgtca gagtcgtaaa attcgccaac gacgattatt cggcgttaca
    18601 cttatcactc atacaaatca aatagcagga ttttgcagtg ttacaacttc ttttagcagt
    18661 ttttattggc ggtggtacgg gaagcgtggc gagatggctg ttaagtatgc gatttaaccc
    18721 gctgcatcag gcgattccgt tggggacgct ggcagcaaac ctgattgggg cattcatcat
    18781 aggaatggga ttcgcctggt tcagcaggat gacgaacatt gatccagtgt ggaaagtatt
    18841 aatcaccacc ggattttgtg gcggtctaac aaccttctca acattttcgg cagaagtggt
    18901 gtttttgtta caagagggcc gctttggctg ggcgttactg aacgttttcg tcaaccttct
    18961 ggggtctttt gccatgacag cactggcttt ctggctgttt tcggcctcaa ccgcacacta
    19021 aagaccaaaa aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca
    19081 gagcgattac gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc
    19141 gctgaccttc agcaagagtt ttaaaaccat tagtctggat tgcagagaag tgtacgaaca
    19201 cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact
    19261 taacgttacc tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg
    19321 ctgtgtcggt taccagtaca ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa
    19381 agtgataaaa gtcgcgttat ttttgatgtt acttatttta gctattgatt ttaaagaagt
    19441 tactaaaact tcatttgtct caaaactgaa agcgcatcca ggcaaagtac acattgccat
    19501 tgttgtaggt acccggaatg taggtcatct gaaaagtcac tgggccataa cccacggagg
    19561 ccaatggcag tagaaccggg agagggatgt aattccagtt atcgcgtgcc gttacgccag
    19621 cggtgaatcc cagacctaaa tgaaaatttt catccgccag cggtcgccag gtactttccc
    19681 atccgtatcc ggcaatcggt tcccatttgt tccacgagtc cttaaatgcc atggcataca
    19741 ggccatgcca gtttcctttt tcatcccatc gcgactgacc aaaaccgcca ccccacggtc
    19801 gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg
    19861 caggaatata taaatcataa tgttcaggct gttgccaggt ttgtgcaata ttttctctaa
    19921 acgttgtcat ccactcatct gcgttagcaa aaactttacc aacgctgatt aactgaataa
    19981 aaacaaagga aaagatagcg acatatttac tcacgttcat ttgtgaccat aaaacattta
    20041 tcaaaaatct acttctagca tagcaaagct acaattaaca taaccttaat agacccaaca
    20101 taaagaataa tctgaatagc tgctcattta tttccgcttg ttcaagttaa atatttccgc
    20161 gcatcgatta aagatgatgc gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca
    20221 agtctttttt gatgacaaaa tgcaatcaag ga
//
LOCUS       DAMZII010000088        19588 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZII010000088 DAMZII010000000
VERSION     DAMZII010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.088"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            39..815
                     /locus_tag="POA77_003103"
     CDS             39..815
                     /locus_tag="POA77_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366491.1"
                     /translation="MPKYLLPAELAEIVCGLLVQPALLNELKTPATHKAFMQDIGELV
                     AKHCGGQIDGVIMPEVRDEDSQADYLTSPDETPYIVVSPGQDMHSLTDNVWRHHARDG
                     WLDHIKGSVEESPDELPTHKECAVRRYTLQRLLLQEETSHANLPVQVVRMCEYSTDDV
                     EQTDSSAEFNVTMALGGHAMLAVKDTDSRLVMALTLLIENGVPSVHVNAGDTDPSVLH
                     IRAAHGGLVLSTDSLNHDFSISPSDQYTYGRPGLLLKAAY"
     gene            965..1741
                     /locus_tag="POA77_003104"
     CDS             965..1741
                     /locus_tag="POA77_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366492.1"
                     /translation="MAKLISTRELATIITALLVKPELLGELDSPEKHRALMEDLGRVV
                     AEHCGGNVTEIALPETDGTAAEGALQNGQPVRYLETKESSPYLIVHPDASLPSVTQNV
                     WMHADHDGWEEHFNGETDALTPEMSEQFRQQVYALLTPESHTTSSEMRLTLQDWQLGE
                     AEIPEEDCQPYQVKVLAENKNVQCEVTNETGTTCFGLILEIDRGVPTLHIDTGSDSLL
                     HIHAAHDGLVLTPDAPSHRFEDAPVDRFSYNSPSLLVPGV"
     gene            1930..2271
                     /locus_tag="POA77_003105"
     CDS             1930..2271
                     /locus_tag="POA77_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366493.1"
                     /translation="MRELLGMAGAEHQASVMYQTFGHLDAKLGEKHKGHFVFINGQHG
                     DLCVVHSEFSSFDEGPGYFSDRADFIWELVKNDGPCSKVGIYRFDGEYALPKRRNGRR
                     FSGSVTCLQAF"
     gene            2372..2635
                     /locus_tag="POA77_003106"
     CDS             2372..2635
                     /locus_tag="POA77_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366494.1"
                     /translation="MTNANANDGKTSPVLPTLEQVYALLKANYKPARFADRDGEIWGH
                     EYSWNLARSSLQDLEKYGKSYVSKHSDRMGDGFSFGPDLVIIR"
     gene            2863..3567
                     /locus_tag="POA77_003107"
     CDS             2863..3567
                     /locus_tag="POA77_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366495.1"
                     /translation="MTMKNTVIPTVTENEMGEVITRHSAYGLVSVSRTSTTGQRLYAS
                     DLSHKEVVTMTFSESEQIERDGVIRHRLAEGRRRSPLLQVSLSPAQWATMITSFGMSD
                     GVPCTINSLIRGDYERQPEIGYIESTRERYERQIREAAEREMAKLHEKLEVLRLLAVK
                     GKAGKRELDEAYQSLLSVINNLPVNLAFTNQLIQESMVNIVSHGKAELEATAMGVAAR
                     LGMKEMSSLASLEEKK"
     gene            complement(3600..4019)
                     /locus_tag="POA77_003108"
     CDS             complement(3600..4019)
                     /locus_tag="POA77_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366496.1"
                     /translation="MSEIAEKPLITRVAPLERIYYCASRAEGLKPDRHEIRMQSQFLL
                     LGVIWLPPALAMAELERLLKGYDADHSRLLENVLETAHQQFLKFCEPQKREGGGVENH
                     VECPRQDDALQCLRFMSTRYALAVDLCAAYVCSRYGR"
     gene            complement(4107..6491)
                     /locus_tag="POA77_003109"
     CDS             complement(4107..6491)
                     /locus_tag="POA77_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDI6366497.1"
                     /translation="MKFCRRPLIVAVYTALLFPLYSQAQLYNYSYTDTRGTKVNAEPT
                     REYLNPGSAITLNLISGLDRYERVTVTRSSDNVQMFQSVTGLVTVADRITAADGSEYY
                     GKSMTLPVLGEGTFSLKSETLDNKSNVVATTSYSFSIDITPPVLPDPMQWIRAGFQYG
                     SLDIFGDRTATQAISLSNVSDARSGLDKAEWFAIDSSGVRRTVNAQLNALTGVVQALA
                     AVAAGSTVAPVSPAYYTVGFRVYDKAGNWTETSHRSGIDRTVPSYIQHQVRNAKTGVW
                     EDYVPGMTIYENPVKFRFKRLKSDSTNFNGTDFGWIDQITNSDSTYFYTEVTVSYPET
                     YVYYERYTKAGLNNNINYSTLKFTLGSGVPEAPRYLSGQFHFKKGNSWTPTDIVRFTG
                     PDCVDMGRVNVEPRPYVQTATIIGLGSCNVAVGESSCLVSMDYCKTSGKGYSPYQIHM
                     KGTGTYANLGGLQSHFYTYWDMNPPVINSIVSNPLDKTVVMLVTDSDVTNDWTTYMWA
                     TNIFNLSLKDTSNKVTTLSPKSVAVLNYQLKQVTFDYSKLPSGKYTATGAATDTYNNT
                     AELKLSSTILHDVTPPVVGIIFEGKPADGSLVKGLENLVITLSDDMTKASLKNITLSG
                     GPTSDKVSVGWYSQGTDKYGLNYPRIFPATDDASSYLLTVVAMDEAGNATTKTSRFRY
                     VPNNLIEFNTIKTLAVGMGLKTSDNQPLAYLRTNSIRKKDGSLITGAQTGTLTVRKDA
                     AFAVSMNGVAVVPGDSKDITIDFGQGDGVLIPIFPATSGNVGESSFMIELPQIQ"
     gene            6742..7344
                     /locus_tag="POA77_003110"
     CDS             6742..7344
                     /locus_tag="POA77_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDI6366498.1"
                     /translation="MKNQAIALAMAMLGVSVPASAQIYESIFTDTNGIEVHAPSTRLL
                     LNPASPVTLTLIAGLDRYVNVKITSATGTQLLNTTTTRTSVSDRLKAGDGSEFYGKKV
                     TLPVLGEGRSVVQVSILDLNQSVVATYSYNWLVDTTPLSVNALTGNAAYGSTAGSVWK
                     MGLEATGQFDFTSTNVTDVNGIEKGMFYVYRSNGSLFSTT"
     gene            7356..7592
                     /locus_tag="POA77_003111"
     CDS             7356..7592
                     /locus_tag="POA77_003111"
                     /inference="COORDINATES: protein motif:HMM:NF023666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HDI6366499.1"
                     /translation="MYHTYSKNSVKGAGIPDSNLDEDFTAKVVIYDNAGNTRTLPLQL
                     FRYDNYLGGPRSAKLRQRGARGFRISGLFSWDDR"
     gene            7748..8344
                     /locus_tag="POA77_003112"
     CDS             7748..8344
                     /locus_tag="POA77_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366500.1"
                     /translation="MVYGLENIRIALADNLTTPRITRLQITGGPTADNVELTWSPAGT
                     NVYAPEYPRLFPSFEPSENYALSVTVSDSQSNSKTYTQKFSYLPNNLVQLHNLRTLSV
                     SSALKTSLNEPLAYLSTNVLRKSNGEIAKDVQSATLTVRKDAAFGIKFNGVQAALGES
                     VEVKIDMGLGDNLLVPIYPAENGKVGTSEFMIQIDELK"
     gene            8736..9617
                     /locus_tag="POA77_003113"
     CDS             8736..9617
                     /locus_tag="POA77_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDI6366501.1"
                     /translation="MSSNNNKLSLVIEESLKDSGEVIQLFPTLKQTIQPKVLLRLGVF
                     VPTLKSTDKGGRKSHHIDATEPLSRLSIVEGEGYSQIQIYGPRLDMDTDFKSWCGIVY
                     ALSNRPLDSEGQLELNFPEFAKFCGFDTKRIDKQLKERFDASLTRIARTNISFIKKIP
                     GTDDQITINMHLVESTYYDSSTGKIIIKPNSKLNTLYRVDGKTRLYLKTLQKLARKES
                     AQALYLYLAELPSTFYRIGFDRLRGRLQLSSHLGNQNATVKKALGQLKEIGFLEYSID
                     KENGDYVLNILKRNMKP"
     gene            complement(10203..10718)
                     /locus_tag="POA77_003114"
     CDS             complement(10203..10718)
                     /locus_tag="POA77_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366502.1"
                     /translation="MKISTGMMKSNAPLIILICLMIAFSAYVKSWSLLYTLLAASVSL
                     TVMISLPFIVSPLQHSKFIALRILRLLVITLWTIGLVGVFFSVVERLVWVNADSYPAW
                     LAKELDSPAITDGENFIKATESFEKICGKNKGYLSVVTKHNGIFMRCDDSLSFDSWWK
                     GVYRLKTPETR"
     gene            complement(11369..13825)
                     /locus_tag="POA77_003115"
     CDS             complement(11369..13825)
                     /locus_tag="POA77_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDI6366503.1"
                     /translation="MRKILTGCIIATAIASVYPAAAQIFSYSYTDTNQLAKTLKPATQ
                     SYLNPASTMTLNLISGLDRYERVSVTRDSDKKVMYSAVTGKISVADRIVAADGTEYYG
                     KDLALPALGEGAFTVINDTLDLQQAVVSSSTYHFTIDTTAPKYTSIYPSQNAGYEMVL
                     SGPVWGLGRGGSGQFSIFADGVDDANGIDKIRLVIKRGNGTVVSDNTLSYDTAAKRAF
                     YPWIKDSVTQPGMPTSDLNEEFTFNFIITDKAGNTLNIPPQRFLFDDQIGEYTPFAVH
                     DSRVSTSVVPGISSGYVPFVRGLSVLENPYRMVIRIPRTNWKPYRNGGITILNNYGGA
                     KVISEDTTYVYVEVKLPQGALDINYYRPVNTYQWSGGDLTQYASWLNWDPASVKSPAW
                     GSPAIERQMSDGTWFNSVNWKYFKASDMPVNLTQIRFNVQARPYDQKITGGATCNIPA
                     GSTSCTVALTQAITNGTTGYLHSGYEVRSTTESTFFMPIWENVVWHTLGPSVTGYDYN
                     DTTNILQVYINQPGDGSYFDTVKLSRVWLSDKSRNNADINVTGTLTGRNTATGNYTYE
                     FNLKLIPEGSYNVQLNAQDSFNNTGSLDYKTVTVDNTPPSISISYETKPITSSVTVYG
                     LENIRIQLADTLTKPSLTRMTLRGGPVSDAVELSWVNLGNNLYAPNYPKIFPSLNDGE
                     TYTLTVQAKDEMNNVKESAVEFSYLPNNLVRLENLKTISVSTALKTSDDTPLAVLYAS
                     QLRKKDGSIATGKQDAILTVRKDAAFGVTVNGVSAAPGESKNVQLDLGLGDSRSFPIF
                     PSTSGVVGTSEFMLNIEELK"
     gene            complement(14271..14447)
                     /locus_tag="POA77_003116"
     CDS             complement(14271..14447)
                     /locus_tag="POA77_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366504.1"
                     /translation="MSKSSPVLPEDRDDIEFVMDDFQRSLGKSKPVREVQFLGESQQS
                     EESANSADSPWFES"
     gene            complement(14462..16138)
                     /locus_tag="POA77_003117"
     CDS             complement(14462..16138)
                     /locus_tag="POA77_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6366505.1"
                     /translation="MSAVESDSLPMGAPGSSLVAYMPHTGLLINDDNFLPEMREELDV
                     INRQVESYNDELSLLRSMSAQFLKCGNQKLYSYLSGYNHLISEADASYAENALRSEYW
                     KRVMALTDVLSVMSDEKKKEWDKQFTADRYHLPPQEIPDFTLDAVVTTVVALLNDRHQ
                     FIKDRVYAVFQSLSRQHKTNKAFGFSTRMITSGVCEGVKDKWVKLKVEFKESGLMPLS
                     ELRVICSYFRGETVKPVYDTKKMVENMVGHVGFRNWISLDGNSIRFRVYKNGSMHIDV
                     HPDIAERLNNILAAIVPLALPAERVAHTKATLQEFPALKRCIDFHSRMQLAELDFTQN
                     EKEWSSWTSLGYAGENADKTRQVNADVLRFLGARVTSYHVTFDYDPTEVMRFIGHMGA
                     MPDIKSHQFYPSSARLSEYVASIIAAGEGERLLEPNIGHGDLLQCFPKSVNVTGIELD
                     TLNCLVSRAKGYETTEADFLAWSAANQIQKFDCVVMNPPFADNRAKLHLLAAASHLVP
                     GGRLAAVLPLSLQGIDNLLGEGFRTEWMDTFDNEFDNTAVSVRVLYAERF"
     gene            complement(16402..17559)
                     /locus_tag="POA77_003118"
     CDS             complement(16402..17559)
                     /locus_tag="POA77_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6366506.1"
                     /translation="MSDLMTDSQQQTNSRVFKSPLVSFSTVTGSGRFVSGMKQFRDAN
                     PDLCNEVTEQKAWAISPTVVQITEGFNSREMGMGEAYYQLPEVADHLNNIKNAYIRGD
                     YVDPIRVLIKDGIPYVRQGHCRLKAALMAVAEGHSNLTILCIEMKADEISCELTTIDG
                     NRGLALSPVALGESYRRLQALGGWSLDRIAEREGKSNTTIAALIRLTGCCVYLKKLIH
                     ADAISYVAVLSLIDEFGETEAVARIEKMIADLEKADSSGIQIKKTPRGQIRVVPSDFK
                     PARVPPVLATKAVEGVKLITTSLLQKLGDIELPEVTDSSEDEEITFTLNRSMLEMLKN
                     LQSEITESENKQLRRAENRQARLKGEKVTYKTKKNDKNGGEPQSETPPAQA"
     gene            17919..18743
                     /locus_tag="POA77_003119"
     CDS             17919..18743
                     /locus_tag="POA77_003119"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDI6366507.1"
                     /translation="MRKHTPIIKWAGGKTKLMPFLSKHFPHDKSRRWVEPFIGGGAVF
                     LNMFATEALLADSNPDLINLYRNIQRNKPAFIREVQLLAERHFEEEDYYVLRNTFNST
                     SFDDAPLQRAAIFYAMNRLGYNGLCRYNLKRKFSVPWGKRYQFSLDIQKVDYLSFRLS
                     SVELKTADFGQTLEFAGSGDQIYCDPPYDKISKTSFVSYDGIPFDKSAHVKLADMLVD
                     ANRKGASVAISNSMTPFTLELYEERGFDIHTHNAYRSVGSQSKSRKKEIEILAVLR"
     gene            complement(18758..19579)
                     /locus_tag="POA77_003120"
     CDS             complement(18758..19579)
                     /locus_tag="POA77_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366508.1"
                     /translation="MQNDLGLGFIRKSARTSAKIISRKVTFPLDDLDNAKLTLTGLQC
                     HPSCRFPFYQLSMLIHNQLAEHGVISADENAPFTVSISIHEWLSRARVAFIDYHGLRN
                     SDKTVMITRAHLEELLTYLSTTLAVYVPYSKKRLNFSFLTSFTLVKTSQSYTLTFPGV
                     LRPFLMLLGGLIQEFMTEKLLKRRNAQFVIFDYLRKSRRHSHKITDIVNDLQLHTVNV
                     RIMNVLTQLADQGFISFICEGKRGERSIEELQFIPYASRDHSEVLSFEEWISPID"
BASE COUNT         5053 a         4778 c         4903 g         4854 t
ORIGIN      
        1 tgctagagtg ttctcattca aaaagaggag aactaaccat gccaaaatat ttgttacctg
       61 ccgagcttgc ggaaattgtt tgtggattac tggtgcaacc agcattattg aatgaattaa
      121 aaacaccagc cacgcataaa gcatttatgc aggacatcgg cgagctggtg gcgaaacatt
      181 gcggcggtca aattgacggc gtgatcatgc ctgaggtacg cgatgaagac agccaggccg
      241 attacctgac atctccagat gaaacccctt atattgttgt ctctcctggc caggacatgc
      301 actccctcac agataacgtc tggcgtcatc atgcccggga tggctggctt gaccatatca
      361 aaggcagtgt cgaagaaagc cctgatgaac tgccgaccca caaagagtgc gctgtacgcc
      421 gctacaccct ccagcgtctg ctccttcagg aagaaaccag ccatgctaat ctgccagtac
      481 aggtggtacg gatgtgtgag tacagcaccg acgatgttga gcagaccgat tcttcagcag
      541 agtttaacgt cacaatggcg ctcggtggtc atgccatgct ggcagtaaaa gatacagatt
      601 cccgtctggt gatggccctg acactcttga ttgaaaatgg agttccctct gtccacgtta
      661 atgcgggtga cactgatcct tcagttctgc acatccgggc agctcacggt ggtctggttc
      721 tttcaaccga ctcccttaat catgatttca gtatttcgcc ctctgaccag tatacctacg
      781 gacgccctgg attattattg aaagccgcct actgatttat taattcaaaa tccgtcatta
      841 ttatttaccc ccatttttcg cccctaactt atggggcgag tatattttaa aaaactttct
      901 ttcaggttat tagccagcaa actagaactg gtagaattaa ttcatcagag aaaaggggac
      961 tatcatggct aaattaattt cgacgcgtga actggcaaca attattactg cattactggt
     1021 aaaaccagaa ttactgggcg agctggattc cccagaaaaa catcgcgccc tgatggaaga
     1081 tctggggcgt gttgtggcag agcattgtgg cggtaatgtc actgaaatcg cactaccgga
     1141 gaccgatggt acggctgctg aaggcgcgtt gcagaatggc cagcccgtcc gctatctgga
     1201 aaccaaagaa agctctccgt atctcatcgt acaccctgac gcctcactgc cgtcggtgac
     1261 gcaaaacgtc tggatgcacg ctgatcatga tggctgggaa gagcatttca acggagagac
     1321 cgatgccctg actccagaga tgagcgagca gttccgccag caggtttatg cactgctgac
     1381 gccggaatcc cataccacct cttcagaaat gagactgact ttgcaggact ggcaactggg
     1441 tgaagccgag atcccagaag aagactgtca gccctaccag gtgaaggtgt tagcagaaaa
     1501 taaaaacgtt cagtgcgaag tgacgaatga aaccggcacc acgtgtttcg gcctcatttt
     1561 ggagatcgac cgtggtgtac cgacgctgca cattgatacg ggcagtgatt cactgctgca
     1621 cattcacgcg gcacatgacg gcctggttct gacaccggat gcaccgagcc acagatttga
     1681 agatgctcct gtagatcgct tttcttataa cagcccgtcc ctgttggtgc ctggggtgta
     1741 accaggcaaa ctgcccagcg gtattttggc cccgggtaaa attattcaga acaggagtca
     1801 ggcagatggg cgatgttgcc acactaccac cgaaaattaa cgatggcgta cttcgacgct
     1861 ggaatgctcg cggtaaagcg gataaagagg cgctggccga ctatggcgtt agcgtgaagg
     1921 aggagtgaat tgcgcgaatt acttggaatg gcaggtgcag aacatcaagc ctccgtaatg
     1981 tatcaaacgt tcggacacct cgacgcgaag ctaggcgaaa agcataaagg tcacttcgtt
     2041 tttatcaacg gccagcacgg cgatctgtgc gttgtgcata gtgaattttc atcctttgac
     2101 gaaggtccag gctatttcag tgaccgtgcc gacttcatct gggaattagt taaaaacgac
     2161 ggcccatgct ctaaggtcgg gatttaccga tttgacggcg aatacgcgct tcctaagcgc
     2221 cggaatggga gaagattttc aggaagcgta acgtgcctgc aagcattcta attccgtgct
     2281 gcatgagcgc cgacaggtct tccgtacaac gggaggcggc acagtgcgcg tgcaaccatc
     2341 cccacacaat aactcgactg agtacataat aatgacgaac gctaacgcaa atgacgggaa
     2401 gaccagcccg gtattaccca ctctcgaaca ggtctatgct ctgctcaagg ccaactataa
     2461 acccgcacga tttgctgatc gggatgggga aatctggggg catgagtatt catggaactt
     2521 ggcaagatcc agtttgcaag acctggagaa atacgggaaa tcatatgtat ccaagcattc
     2581 cgaccggatg ggcgatggat tcagttttgg tcctgacctg gtcattattc gctgacatac
     2641 gcacggaagt gagtaagatg gctggctgcg ccggagaact agagtaatga agggtaaaaa
     2701 tatcgtggct cgtgtcatgc gggaagcggc ctataagtgg gaaactcgcc accaattgca
     2761 gcaggcgtac aattcagcga gtgacgtggg aaaagttccc agacattccg gttccgcatc
     2821 tgatttacgc cagacgctgt catttttaat tctttgtagg gcatgacgat gaaaaacaca
     2881 gtaataccga cggtgactga aaacgagatg ggagaggtga tcacccgcca ctcggcttac
     2941 ggactggtat cagtaagccg cacgtccacg acgggacagc gcctttatgc ctccgacctc
     3001 agtcataaag aggtagttac gatgaccttc agtgaatcag agcagattga acgcgacggt
     3061 gtgatccgtc atcgactggc ggaaggccga cgccgctcac cgctcctgca agtcagtctg
     3121 tctccggcgc agtgggccac gatgatcaca tcgttcggaa tgagtgacgg cgtgccgtgt
     3181 accattaaca gcctgattcg cggcgattat gagcggcagc cggagattgg ctatattgag
     3241 tcgacacgcg aacgatacga acggcagatc cgggaagccg ctgagcgtga aatggcgaaa
     3301 ttgcatgaaa agctggaagt gctgcgtttg ctggcggtaa aaggcaaggc tggaaagcgt
     3361 gagcttgacg aagcctatca gtcgctgtta agcgtaatca ataacttgcc ggtcaatctg
     3421 gccttcacca accaactcat tcaggaatcc atggtgaata tcgtctcaca cggcaaggcc
     3481 gaactcgaag ccaccgccat gggcgtagcc gctcgtctgg gtatgaaaga aatgagcagc
     3541 cttgcctcgc tggaagagaa gaagtaaccg gtgcgcccgc ccgtaaatca tgaaatgcac
     3601 taacgtccat acctgctgca cacatacgcg gcacacagat caacggccag cgcataccgc
     3661 gttgacataa accgtaaaca ctgaagtgcg tcgtcctgtc ggggacattc tacgtggttt
     3721 tctacgcccc ccccttctct cttctgcggc tcacagaact tcaggaattg ctggtgggca
     3781 gtctccagga cgttttccag taaccggctg tggtcagcgt catatccctt cagcaggcgt
     3841 tccagttctg ccatagcgag tgcaggtggt aaccagatga cgccaagcag caaaaactgg
     3901 ctctgcattc gaatttcatg gcgatctggc ttcagaccct ctgcccggga agcgcaataa
     3961 taaatcctct ccaggggagc tacacgcgtg ataagaggtt tctcagcaat ttctgacatg
     4021 cgactaaatc agcctgtttc gtttctttct gattatgtta agacactatg aaaaagcccc
     4081 gtaatacggg gccggttcgg aggcagttac tggatttgcg gcagctcgat catgaagctt
     4141 gattcaccga cattgccgct ggtggccggg aagatgggga tcaggacgcc atcaccctgc
     4201 ccgaaatcta tcgtgatgtc tttcgaatcc ccgggtacga cggccacccc attcatactg
     4261 accgcaaagg ccgcatcttt gcggaccgtc agcgtgccgg tttgtgctcc agtgatcagc
     4321 gaaccatctt ttttccggat gctattggta cgcagatacg ccagcggctg gttatctgat
     4381 gtcttcagtc ccatgcccac ggccagcgtc ttgatggtat taaattcaat caggttgttc
     4441 ggtacatagc ggaaccggct ggtctttgtg gtggcgtttc cagcttcatc catagctacg
     4501 acagtcaaca ggtaggaact cgcgtcgtct gtcgcgggga agatacgcgg gtagttcagt
     4561 ccatatttgt cggttccctg gctgtaccag cctacgctga ctttatctga cgtgggtccg
     4621 ccgctcagcg taatgttctt cagagaagct ttagtcatgt catcgctcag ggtgatcacc
     4681 aagttttcca ggcctttgac aagcgatccg tcagctggtt ttccttcaaa gatgataccg
     4741 acgactggcg gcgtcacgtc atgcaggatg gtgctcgata gcttcagttc agccgtgttg
     4801 ttgtaggtgt ccgtcgcagc acccgttgca gtgtatttcc cggaaggtaa cttactgtag
     4861 tcaaacgtca cttgcttcag ctggtagttc aggactgcga cgcttttcgg cgacagggtt
     4921 gtgactttat tactggtatc cttcaatgag aggttgaaaa tgttggtggc ccacatatag
     4981 gtggtccagt cgttagttac gtcgctgtca gtcaccagca taacgactgt tttatctagt
     5041 gggttggaaa caatactgtt gatgacagga ggattcatat cccagtaggt atagaaatgc
     5101 gactgaagtc caccaagatt agcgtaagta ccggtccctt tcatatgaat ttggtacgga
     5161 ctatagcctt taccgctcgt cttacagtaa tccatgctga ctagacaact gctctccccc
     5221 actgcaacat tgcagctacc aagacctatg atagtggcgg tttgcacata cgggcgtggt
     5281 tcaacgttaa cacggcccat atcaacgcaa tccggtccag tgaacctgac aatgtcggtc
     5341 ggtgtccagg aattgccttt tttgaaatga aactgcccgg ataaataacg tggagcttcc
     5401 ggcacaccgc tgccgagagt aaatttcagt gtactataat tgatattatt gttcagacca
     5461 gccttggtat accgctcgta gtaaacatag gtctctgggt aactcaccgt gacttcagta
     5521 taaaagtagg tgctgtcaga attagtaatc tgatcgatcc agccaaaatc agtcccgtta
     5581 aaatttgttg agtccgattt aaggcgtttg aaccggaatt ttaccgggtt ttcataaatg
     5641 gtcatgccag gaacataatc ttcccagacg cctgttttcg cattccgcac ctgatgttga
     5701 atgtagctgg ggacggtacg atcaatacca gagcgatgtg atgtttctgt ccagttcccg
     5761 gctttgtcat aaacccgaaa acctacggtg taatatgccg gactgacggg cgcaaccgtg
     5821 cttcctgccg ccaccgccgc cagtgcctgg accacccccg tgagtgcatt cagctgagca
     5881 tttacagtcc ggcggacacc gctgctgtca atagcaaacc actccgcttt atccagtcct
     5941 gacctcgcat cagagacgtt cgataacgaa atggcctggg ttgcggtcct gtcgccgaaa
     6001 atatccagac ttccgtactg aaaaccagcc ctgatccact gcatcggatc tggtagaacg
     6061 ggaggagtga tatcgatgct gaaagagtag ctggttgtcg ccacgacatt cgacttattg
     6121 tcgagtgttt cggatttaag agaaaaagta ccctcgccca gtaccggcag tgtcatggac
     6181 ttgccgtagt actcactacc atcagcggca gtgatcctgt cggccactgt caccaggccg
     6241 gtgacactct ggaacatctg cacattatcg gatgaacgtg tcacggtaac gcgctcgtag
     6301 cggtcgagac cagatatcag attcagcgta attgcgctgc ccgggttgag atactctctg
     6361 gtgggttcag cattcacttt cgtgccgcgt gtgtcggtat acgaatagtt gtaaagctga
     6421 gcctggctgt aaagcgggaa cagcagtgcc gtatagacgg cgacaatcaa cggacgtcgg
     6481 caaaatttca tttcggttct cctggttggc cagaaaattt tacctgaatc gttttcttcg
     6541 cccgggcgct aagaggggta attgttcagt aaaacatcgt ccgtttgtgt gctttttgcc
     6601 agatttaatc ggcaggcctg cgggaaatac cccatcccaa tgtccgtttt aggctgatta
     6661 attccccaga aatccccccc ttaacggaaa aatccgcagg gcagacgttt aaaatagcaa
     6721 caactaaaag gggaatactg aatgaaaaat caggctatag ctttggctat ggctatgctc
     6781 ggagtctcag ttccggcatc tgctcaaatt tatgaatcca tcttcacaga taccaatggt
     6841 attgaagttc acgccccctc aacccggctg ttgctgaatc cggcctcacc ggtaaccctg
     6901 acgctcattg ccggtcttga ccgttatgtg aatgtcaaaa tcaccagcgc caccggcacg
     6961 caactgctga acaccactac tacgcgcacc agcgtatccg accgtctgaa ggccggagat
     7021 ggcagcgaat tttacggaaa aaaagtaact ttaccggtgc ttggcgaagg caggtcggtt
     7081 gtgcaggtca gtattctgga tctgaatcag tccgttgtag ccacctacag ctacaactgg
     7141 ctggtagaca ccacaccgct gtctgttaat gccctcacag gcaacgccgc ctatggatca
     7201 actgcgggta gcgtctggaa aatggggctg gaggccacgg ggcagtttga ttttacctca
     7261 accaatgtca cggacgtaaa tggcatcgag aaaggtatgt tttatgtcta tcgcagtaac
     7321 ggcagtttgt tcagtacgac gtgactggtc agaagatgta tcacacttac tcaaaaaact
     7381 cggtcaaagg cgcgggtatt cccgacagca atctggatga agatttcacg gccaaggtag
     7441 tcatttatga caacgcagga aacacccgaa cccttccgtt gcagctcttt cgatatgata
     7501 actatctggg cggtccgaga tccgcaaagc tccgccagcg tggtgccagg ggtttccgga
     7561 tatccggcct attcagctgg gatgaccgtt aatgagaatc cgattcatct tgtatatcgc
     7621 gtgccaaagt ccaactaccg gtcatatgcc gagggcggcc tgctattcta taacctgtat
     7681 tcttccccga ccgagattgc gacggacagc acctatacct acgttgaaat gatcctgccc
     7741 tagtacggtg gtatacgggc tggagaatat ccgtatagct ctggcggata acctgacaac
     7801 accccggatc acccgtcttc agataaccgg agggccaacg gcagataacg ttgagctcac
     7861 gtggtcacct gctggcacga acgtttatgc gccagaatat ccaaggctgt tcccctcatt
     7921 cgagccttcc gaaaactatg cgcttagcgt gacggtctcc gacagtcagt cgaacagcaa
     7981 aacttatacc cagaaattca gctacctccc gaataaccta gtgcagttgc ataacctgcg
     8041 aaccttatcc gtcagttcag cactcaaaac gtcgttaaat gagcctcttg cttatctgtc
     8101 aaccaacgtg ttgcgtaagt ccaacggtga gattgcaaaa gacgtccaga gtgcgacgct
     8161 aacggtacgt aaagacgctg ccttcggtat taagttcaac ggcgtgcagg ccgcgcttgg
     8221 tgaatctgta gaggtgaaaa ttgatatggg gctcggtgat aaccttttag tacctatata
     8281 ccctgctgaa aacggcaaag tgggtacatc cgagttcatg atccagatcg atgagctgaa
     8341 gtaatgagaa tgggccgtcg gtagtactgg cggcttaacg aaagtaactt tttctgtaag
     8401 ggttgaagtg tgcttctgat gtttttagaa cggatcggaa tttactcaca ttttgatcgt
     8461 gatgattcaa tacgtgacag agcatagtta tcaacaggat agaataataa taaagatcaa
     8521 tagaggatca atagcagatc aaaaaggaat aacgatcctc gcagcccagt gatatcgggc
     8581 ctctcagcgg atcagcatcc actgtacagg gggagaaata cactatgtcg ggtaatccat
     8641 ccactgtacc gggcgcataa tccactatac cgggtagatt cgatacacta tagcgggtag
     8701 gatgtagatc ttatgcacaa aacggatctc ccaatatgtc aagtaataac aacaagttat
     8761 ctctcgtcat tgaagagtcc ctaaaagatt caggggaagt tatacaactc tttcccacat
     8821 taaaacagac cattcaaccg aaagttttac tccgcctagg tgtgtttgtg ccgactttga
     8881 aatctacgga caaggggggg aggaagagcc atcatatcga tgccactgag ccgctatcaa
     8941 ggttatctat tgtagagggt gaaggttaca gccagatcca aatctacggg ccgcgtctcg
     9001 atatggatac cgatttcaaa tcatggtgtg ggatcgttta tgcgttgtct aaccggccgc
     9061 ttgattcaga aggtcagctg gagctgaatt ttccggagtt tgcaaaattt tgtgggtttg
     9121 ataccaagcg cattgataaa caactgaaag agcgctttga tgcttcgctg acgcgtatag
     9181 cccggaccaa catttcattt attaagaaaa tacccggtac agatgatcag ataacgataa
     9241 acatgcacct ggtggaatcg acgtattacg acagttccac gggaaaaatc atcatcaagc
     9301 caaattcgaa gctcaatacg ctgtatcgcg tcgacggtaa aacccgactc tacctgaaga
     9361 cattgcaaaa actggcccgt aaggagtccg cacaggccct gtacctgtat ctggctgagc
     9421 tgccgagcac cttttaccgc atcggattcg atcggcttcg tggacgactc caactgtcat
     9481 cacacctcgg taaccagaat gccacggtta aaaaggcgct gggccagctt aaagagatcg
     9541 gttttctgga atactccata gacaaagaaa acggcgatta cgtactgaat attctaaaga
     9601 gaaacatgaa gccatgaccg atgccggata atgcacctgc ctgccgcgtg ggtggctgcg
     9661 ttacccggta tagtggacat tcttctcctt tccactctgc tttacccggt acaatggatc
     9721 tccactacac cgggtagtgt caggcctaaa attacgatgc agcccctcct cttcagcctt
     9781 acactgaata tttagtgtgt tttttcacca aaaatgtgat ctatacccgg tacagtggag
     9841 tcacagaaat gtgatccgta cccggtatag tggatgagaa ctactgctaa tcggttaccg
     9901 ggaatgtggt ggtactgggt ggaaaggttt acccggtata gtggatcgtg gcagtgtcat
     9961 ctttccggat agcatgacgt cacggttagg aaaccgtacc cggtatagtg gatagcgaat
    10021 ggtgtacaac cctgttaacg tgatgatgct gaagtgaaag tttaacccgg tttagtggag
    10081 cgacttaaag cgcgtaccag tagctgatgg gggagcagca cccggtacag tggaggggcg
    10141 gatatcgtcg taaaggtctt aactaaaaga tgaccattca gtcgcagcat taaagaaata
    10201 tatcatcgag tctcaggggt tttaagccgg tagacaccct tccaccagga gtcgaaagac
    10261 aagctatcat cgcagcgcat aaatatgccg ttatgcttgg tgacaacgct aagatagcct
    10321 ttatttttcc cgcagatctt ctcaaaagac tctgttgctt taataaaatt ttcaccatcc
    10381 gtgatggccg gagaatccag ttccttcgcc agccatgccg gatagctgtc cgcattaacc
    10441 catacaaggc gttcaacaac tgaaaagaag acgccgacaa ggccaatcgt ccaaagcgta
    10501 ataaccagta aacgcaaaat tcgtaaggct ataaattttg agtgctgtag aggcgaaacg
    10561 ataaaaggca gtgagatcat gactgtgagt gagactgatg ccgccagcaa ggtgtataac
    10621 aatgaccatg atttaacata ggcggaaaaa gcgatcatta aacaaatcag aatgatgagc
    10681 ggtgcattgc ttttcatcat gcccgtgcta attttcattc ttttggggct ccattttgta
    10741 cgagttttga gagactgcgt ttgtaatcat ccgctgcctt agaaaagtta ccgtcattct
    10801 gtaccggcgt aaagcgaatg ccaaaaaacg cctcaatgac ggaggaaagc gtcactatcg
    10861 gtcctgacga ggcatgggct tttcgctccc gagcctcacg ctccgcaaag tggatcaccg
    10921 gtcccctaga aaatgagagt gagtcatggg aagaccagtc tgtttatagc cacagtagtt
    10981 aaacgcaccc cgccagtccg tcgctgtttg acatcatggc cgcgaattag gttaacgtcg
    11041 cccggtatag tgtaatccca ggctgaacct gtgtttgcag ctggtggccc tgcatccatc
    11101 accatgacag gcgctggccg caggaatcgc gtgccggatg aagggtatcc actataccgg
    11161 gtgaatgata tgttgatcca cgctgacggg tagttttatg agcaaacccg cagcgagtgg
    11221 caggcctgtg tcattcgcag ttttactgat gggacggaag gttatccaca aaaacggtga
    11281 ataaccctgt gaccggaatc tggataatca tcattgcggc gacaacaaca gaaagaagtg
    11341 gcccggactt ggccacccta ccctgctttt acttcagttc ctctatattc agcataaatt
    11401 cagaagttcc aacgacgccg gaagtcgaag gaaaaatagg gaagctgcgg ctgtcaccca
    11461 gacccagatc caactgaaca tttttgctct ccccaggagc tgcggaaacc ccattcaccg
    11521 tgacgccgaa ggctgcatcc ttacgcaccg tcagtatcgc gtcctgtttc ccggttgcga
    11581 tagacccgtc cttcttacgc agctggctgg cataaagaac cgccagcggt gtgtcatcgg
    11641 acgttttcag cgcggtacta acggagatgg tcttaaggtt ttccagacga accaggttgt
    11701 tcggcaagta actaaattcg acggcgcttt ctttaacgtt gttcatctcg tctttggcct
    11761 gcacggtaag cgtgtaagtt tcgccgtcgt taagtgaagg aaagatcttc gggtaattcg
    11821 gggcataaag attatttccg aggtttaccc acgacaactc aacagcatca gataccgggc
    11881 caccgcgaag cgtcattcgc gttaaagagg gcttggtcag ggtatctgcg agctgaatac
    11941 gaatgttctc cagtccatac accgttacac tgctggtgat aggtttggtt tcgtagctga
    12001 tactgatgga cggcggcgtg ttatcgaccg tcacagtctt atagtccaga ctacccgtat
    12061 tgttgaacga atcctgggcg ttgagctgca cgttatagct gccctccggg attagtttca
    12121 ggttgaactc gtatgtataa ttcccggttg ctgtgttacg ccccgttagt gttccggtca
    12181 cattgatatc cgcgttattg cgggatttgt cggacagcca cacccggctc agtttaacgg
    12241 tatcgaaata cgatccgtct cccggctggt tgatgtaaac ctgaaggatg ttggtcgtat
    12301 cattgtagtc atagccagtg accgaaggcc ccagcgtatg ccagacgacg ttttcccaga
    12361 taggcatgaa gaaggtggat tctgttgtgg aacgtacctc ataaccacta tgcagatagc
    12421 cggtggtgcc gtttgtaatc gcctgtgtca gggcgacggt acacgacgtt gacccggcag
    12481 ggatgttgca ggtggcaccg ccagtaattt tttggtcgta gggccttgcc tgcacattga
    12541 aacggatctg ggtcaggttc accggcatat ctgaagcctt gaagtatttc caattgacgc
    12601 tgttaaacca ggtaccatcg gacatctgtc gctcaatcgc cggactcccc catgcaggac
    12661 tttttaccga agccgggtcc cagttcagcc aggaggcgta ttgagtcaga tcgcctccgg
    12721 accattggta cgtgttcact gggcgatagt agtttatatc cagagcgccc tggggaagct
    12781 tcacctcgac gtacacatag gtcgtgtctt ctgaaataac cttagcaccg ccataattgt
    12841 taagaatggt gatgccgccg ttacggtatg gcttccagtt ggttcggggg atacggatga
    12901 ccatacggta tgggttttcg agtacagata gcccccgaac aaacgggacg taccctgaag
    12961 agatccccgg gaccacactc gtactgacgc gtgagtcatg aaccgcaaag ggggtgtatt
    13021 cacctatctg gtcatcaaat aaaaagcgct gcggcggaat attcagcgtg tttccggctt
    13081 tatctgtgat gataaaattg aaggtaaatt cttcattgag atcgctggtg ggcataccgg
    13141 gttgagtgac cgagtccttg atccaggggt aaaaggcacg ttttgccgcc gtatcgtaac
    13201 tgagtgtgtt gtcggacacg actgtgccat ttccgcgctt gatgaccaga cggatcttgt
    13261 ctatgccatt cgcatcatca actccatcag caaagatgga aaactggcca gatcctccgc
    13321 gcccaagccc ccaaactggg ccactcagga ccatttcata gcctgcgttc tggctcgggt
    13381 agatgctggt atactttggc gctgtagtgt cgattgtgaa atgatacgtt gaagagctga
    13441 cgacagcctg ctggagatcc agagtatcgt tgatgacggt gaaggcccct tcaccgagtg
    13501 cgggtaacgc caagtccttt ccgtaatatt ccgtgccgtc tgcggcaaca atgcgatctg
    13561 cgacgcttat ctttcctgtc actgcggaat acatgacctt tttgtcactg tccctggtca
    13621 cactcactcg ttcgtatcgg tcaaggccgg agatcagatt cagggtcatt gtgcttgccg
    13681 gattaaggta gctctgcgtg gcaggcttaa gcgtctttgc cagctgattg gtgtcagtat
    13741 acgaatagct gaaaatctga gccgccgccg gataaactga agctatggcc gtggcaatga
    13801 tacagcctgt cagaatttta cgcatgtgat actcccggga tgtatttgcc cctattttac
    13861 gaaatctttt cacctgggct tgctggttag ccggggtatt tcacggcata aaacttttaa
    13921 atgttgccgt gatagcaatg ttgtcgggtc agaacaaaac gaaagttaag gcaacttcaa
    13981 cagggctgat tacggtggaa gaaaagtaac ttttctcttg agaagaaata ggaaaagcag
    14041 aaattaggac gattaaatga ggtgaaatgt cagcaacagg cgatgattgt ccatcatcgt
    14101 aagggagggt acagggtgcc gctgccttac tggcatcatg ttgatgtcca gctcttcata
    14161 cccgtttgta ccttagccgg aactgttgtt cagtacaggt aaaagttgta ataactcccc
    14221 gctcactgcc ggggagtcat tttaaatcag cacggcgcca tcagaataga ttagctttca
    14281 aaccagggcg aatcggcaga gttcgcagat tcttcacttt gctgtgactc tcccagaaat
    14341 tgcacttcac gtactggttt acttttcccc aggctccgct ggaaatcatc catcacaaac
    14401 tcaatatcat cacggtcctc aggcaagaca ggcgaacttt tggacatagt ctttccttca
    14461 gttaaaagcg ttctgcatac agaacgcgga cggagacggc agtgttgtca aactcattat
    14521 cgaaggtatc catccactcg gtcctgaacc cctcgcccag caggttatcg atcccctgaa
    14581 gggacagagg caggacggca gctaaacgcc cgccgggaac cagatgagac gcagcggcca
    14641 gcagatgcag cttagcccgg ttgtcagcaa aaggagggtt catcaccacg caatcaaatt
    14701 tctgaatctg attcgccgcc gaccaggcca gaaaatcggc ttccgtggtc tcgtaacctt
    14761 tagcccggga gaccagacaa ttcagtgtat ccagttcgat gccggttacg ttgacacttt
    14821 tcgggaaaca ttgaagcaaa tccccgtgcc caatattggg ctccagcagc cgttccccct
    14881 ccccggccgc aataatggat gcgacgtatt cactgagtcg cgccgacgac ggataaaact
    14941 ggtgggattt aatatcaggc atggcgccca tatgtccgat gaagcgcatc acttcggtcg
    15001 ggtcataatc aaacgtcaca tggtagcttg tcactcgggc accgagaaat cgcagaacat
    15061 ccgcgttcac ctgccgcgtt ttatcagcgt tctcaccggc atagccgagc gatgtccagc
    15121 tggaccattc cttttcattc tgggtaaaat ccagctctgc aagctgcatc cgtgaatgaa
    15181 aatcgatgca gcgtttcagt gccgggaact cctgcaacgt cgctttggta tgggccacgc
    15241 gttcagcagg cagagccaga gggacgatgg cagcaagaat gttgttaagc cgttcggcga
    15301 tatcaggatg cacatcaatg tgcatcgatc cgtttttgta gacccgaaaa cgaatactgt
    15361 tgccatccag gctgatccag tttctgaatc caacatgacc aaccatattc tcaaccattt
    15421 ttttcgtgtc atagaccggt ttaactgttt cgccccggaa ataagaacag atcacccgca
    15481 actccgaaag tggcatgagg ccggattctt taaactcaac cttcagtttt acccatttgt
    15541 ctttcacgcc ttcacatacg ccggaggtga tcatgcgagt agagaagcca aatgctttat
    15601 tggttttgtg ttgacggctt aatgactgaa agacggcata gacacgatct ttaatgaact
    15661 gatgccggtc attgagcaac gcgacaaccg tggtaacaac agcatcgagt gtaaaatcag
    15721 ggatctcctg aggaggaagg tggtaacggt cagcagtaaa ctgcttatcc cattctttct
    15781 tcttttcgtc agacatcaca gacagtacat cggtcagggc catgaccctc ttccagtatt
    15841 ctgaacgcag cgcgttttct gcgtagctgg catccgcttc tgaaatgaga tgattgtatc
    15901 cggagaggta tgaatagagc ttctgattcc cgcatttcag gaactgagcg gacatggacc
    15961 tcagtaagct aagctcatcg ttatatgatt cgacctgcct gttaatgaca tcgagctctt
    16021 cccgcatttc gggcaggaag ttatcgtcgt taataaggag gccagtatga ggcatataag
    16081 caaccagaga actcccaggg gcacccattg gcagggaatc agattcaaca gcagacatag
    16141 caccagtcct ttttgaaggg tttatctgat accaggataa caaccttgaa caaaaagcta
    16201 ataggttggc tgacatgaaa atggagaaat taacactgcg gaaagtcaga aagacagatg
    16261 tggttttaat cagaagaaac gggccggaga caggacaaaa aaacccctac atagtagggg
    16321 caacgggggg agagcctggg tggctcgctc taaaatatcg tacttcttat tgtagtcttc
    16381 tcacactctg aaataattca tttaagcctg tgctgggggt gtttcggatt gcggttctcc
    16441 gccattttta tcgtttttct tggttttgta agtcactttt tcccctttca atcgggcttg
    16501 ccgattttcg gcccggcgaa gctgcttgtt ttctgattcc gtaatctcac tttgcaggtt
    16561 cttgagcatc tctagcatgc tccggttcag cgtaaaggta atttcttcat cttcgctgga
    16621 gtcggtcacc tccggcaact cgatatcacc gagtttctgc aatagactcg tcgtaatgag
    16681 tttgacccct tcgacggctt ttgtagcgag aactggtgga acacgtgcgg gtttaaaatc
    16741 ggaagggaca acacggatct gcccgcgtgg cgttttctta atctggatac cgcttgaatc
    16801 agccttttca aggtctgcga tcatcttttc gatccgcgca acagcttccg tttcaccgaa
    16861 ctcatcgata agggacagga ctgctacata ggaaatggca tcggcatgga tcagtttttt
    16921 gagatagaca cagcagccgg ttaaacgaat aagagcagca attgtggtat tactttttcc
    16981 ctcacgttcg gcaatacggt caagcgacca tccacccagc gcctgaagac gtcggtagga
    17041 ttcacccaaa gccacaggag aaagagccag gccacggttt ccgtcaatcg ttgtcaattc
    17101 gcagctgatc tcatcggctt tcatctcgat gcaaagaatg gtgaggttag aatgcccttc
    17161 tgccacagcc atcaaagcag cctttaaacg gcaatgtccc tggcgaacgt acggaatgcc
    17221 gtctttgatc agaacgcgga tagggtctac gtaatcaccc cggatgtacg cattcttgat
    17281 gttattgaga tgatcggcga cttctggcaa ctggtaataa gcttccccca ttcccatctc
    17341 acgggagttg aatccttccg tgatttgaac cacggttggg ctgatagccc atgctttctg
    17401 ttcagtgact tcattacaaa ggtcagggtt cgcgtcgcgg aactgcttca taccactgac
    17461 gaatcggccg gaaccggtca ctgttgaaaa agacacgagt ggggatttaa agacccgtga
    17521 gttagtttgc tgttgggaat cagtcatcag atcactcata gatactccgt tgaattcttt
    17581 tttgtcatac ctttcatctg tgcgtgaata ctaccggaaa aaaaaatctc actttttcgt
    17641 taaaaaggta ctttttgggt ctattccgaa ttgaacgagg aaaattgttc tggcgatcag
    17701 aacaaaaaga aaaaataact caaataaaac agatggttat ggttaattcg taaaggataa
    17761 acttttttct gagaagtgaa aaaaaattaa aaaatgtgtc gaaatgacaa tctcgttaac
    17821 aaaagccacg ttttttcctg ctcatacctt attggtagca atccgccttt cggtaccata
    17881 agtgtggcat accgataaag ctgaacggag aagactcgtt gcgaaaacat acaccaatca
    17941 tcaaatgggc ggggggtaaa accaaactga tgccgttcct cagtaagcac ttcccacatg
    18001 ataagagccg acgctgggtt gaaccgttta tcggcggtgg cgccgtgttt ctgaacatgt
    18061 ttgccaccga ggctctccta gcagacagca acccggacct aatcaatctg taccgcaaca
    18121 tacagcgaaa taaaccggca ttcatcagag aagtgcagct gctggcggaa aggcattttg
    18181 aagaagagga ttactacgtg ctgcgtaata cctttaacag caccagtttt gatgatgccc
    18241 cgctacaacg agcagccatt ttctacgcga tgaatcgact cggatataac ggtctgtgtc
    18301 gttacaacct gaagcgaaag ttctcagttc catggggaaa gcgataccag ttcagtctgg
    18361 atatccagaa ggttgattac ctctcattca ggctttcgag cgttgaattg aaaaccgcag
    18421 attttggtca gacgctggag tttgcaggta gtggtgacca aatatattgc gatccgccat
    18481 atgacaaaat cagtaaaacc agctttgtaa gctatgatgg tataccgttc gataagagcg
    18541 cacatgttaa actggcggat atgctggtgg atgctaatcg taaaggggcc tcagtggcca
    18601 tatccaacag catgactccc tttacgctgg agctctatga agaacgcggt tttgatattc
    18661 atactcacaa cgcttatcgg tctgtcggca gtcaatcaaa gtcgcgtaag aaggaaatag
    18721 aaattctggc cgtccttcgt tgagcggcga tcagtagtta gtcgatcggg cttatccatt
    18781 cctcgaagct cagcacttcg gaatggtcac ggctggcata gggaataaac tgaagctctt
    18841 caattgatcg ctcccctctc ttaccttcgc atataaacga tatgaacccc tgatctgcaa
    18901 gttgggtaag gacattcatg attcgtacgt tcactgtatg caactgaaga tcattcacga
    18961 tatcggtaat tttgtgtgaa tggcgccggg actttcgcaa ataatcaaag atgacaaact
    19021 gggcattccg tcgcttcaat aatttttccg tcataaattc ctgaattaat ccccccagca
    19081 acatcaggaa cggacgaagt accccaggga acgttaacgt ataagactgc gatgttttga
    19141 ccagagtgaa ggacgtcagg aagctgaagt ttagtcgctt ctttgaatac gggacataaa
    19201 cagccaatgt cgtagacaag taagtaagca attcttccag atgagcgcgt gtgatcatga
    19261 ccgtcttatc gctattacga agaccgtgat aatcaataaa tgccactctg gcacgactca
    19321 gccactcgtg aatgcttatg gatacagtga acggtgcgtt ttcatcggca gaaatgacgc
    19381 cgtgctcagc gagttggtta tggataagca tagacagctg gtaaaatgga aaacggcagg
    19441 atggatgaca ctgtaggcca gttagcgtta gctttgcatt atccaagtca tcgagcggaa
    19501 aagtaacttt tcgggaaata atttttgctg acgttcttgc tgacttgcgg atgaacccaa
    19561 gtccaagatc attttgcata tatcactc
//
LOCUS       DAMZII010000089        19582 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZII010000089 DAMZII010000000
VERSION     DAMZII010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.089"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            233..733
                     /gene="tssB"
                     /locus_tag="POA77_003121"
     CDS             233..733
                     /gene="tssB"
                     /locus_tag="POA77_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDI6366509.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            768..992
                     /locus_tag="POA77_003122"
     CDS             768..992
                     /locus_tag="POA77_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366510.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1043..2518
                     /gene="tssC"
                     /locus_tag="POA77_003123"
     CDS             1043..2518
                     /gene="tssC"
                     /locus_tag="POA77_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDI6366511.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYHEDVSRNHEDYL
                     WGNTAWMLACNVADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2525..2938
                     /gene="tssE"
                     /locus_tag="POA77_003124"
     CDS             2525..2938
                     /gene="tssE"
                     /locus_tag="POA77_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDI6366512.1"
                     /translation="MIPDRERTTGSLFERMEASPARNWQGSSIHALRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSLAIAQCIERYEPRISHAEVQAVVSSATSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2942..4792
                     /gene="tssF"
                     /locus_tag="POA77_003125"
                     /pseudo
     CDS             2942..4792
                     /gene="tssF"
                     /locus_tag="POA77_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            4756..5838
                     /gene="tssG"
                     /locus_tag="POA77_003126"
     CDS             4756..5838
                     /gene="tssG"
                     /locus_tag="POA77_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDI6366513.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5863..7143
                     /gene="tagH"
                     /locus_tag="POA77_003127"
     CDS             5863..7143
                     /gene="tagH"
                     /locus_tag="POA77_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDI6366514.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7140..7664
                     /gene="tssJ"
                     /locus_tag="POA77_003128"
     CDS             7140..7664
                     /gene="tssJ"
                     /locus_tag="POA77_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDI6366515.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7667..8998
                     /gene="tssK"
                     /locus_tag="POA77_003129"
     CDS             7667..8998
                     /gene="tssK"
                     /locus_tag="POA77_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDI6366516.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9003..9764
                     /gene="icmH"
                     /locus_tag="POA77_003130"
     CDS             9003..9764
                     /gene="icmH"
                     /locus_tag="POA77_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDI6366517.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9773..12532
                     /gene="tssH"
                     /locus_tag="POA77_003131"
     CDS             9773..12532
                     /gene="tssH"
                     /locus_tag="POA77_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDI6366518.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESEQDNTGAKLADEAGSEQPEETAETVSPVQRLAQLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLTTIIDGKLARLDNVLRSRFDADVIIESEVTDEIMSRVTRAEN
                     GARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDALDDESVTE
                     DETDL"
     gene            12529..13272
                     /gene="tagO"
                     /locus_tag="POA77_003132"
     CDS             12529..13272
                     /gene="tagO"
                     /locus_tag="POA77_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDI6366519.1"
                     /translation="MSRLRVFRQTGWFIAGLVAGLPASAAPAETSSLAGVEVAVATAT
                     PPDATATLHAMQSCRQETAALERLDCYDRLLAPLSPSGFNGALVKASFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLMVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13277..14692
                     /gene="tssA"
                     /locus_tag="POA77_003133"
     CDS             13277..14692
                     /gene="tssA"
                     /locus_tag="POA77_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDI6366520.1"
                     /translation="MAMDLRDPNVWVSHLLENLPEEKLASALKDDNPDWEYIDGGIVK
                     LGSLAHSQLDIPELQHRGLVLLASESKDFRLLAHLLRTLQHAGDPLLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIDAFSQNAATAQRDALLGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPAPTPAASSSSPQTIATSESGVTHTSTPTPQIA
                     IDSHDDKTWRDTLLKVAAILCERQPESPQGYRLRRHALWLTITSTPQAESDRRTPLAA
                     VSADMVADYQSRLASADMELWQQVEKSVLLAPYWLDGHYISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLANQPGNQSAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            14801..18235
                     /gene="tssM"
                     /locus_tag="POA77_003134"
     CDS             14801..18235
                     /gene="tssM"
                     /locus_tag="POA77_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDI6366521.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18246..>19582
                     /locus_tag="POA77_003135"
     CDS             18246..>19582
                     /locus_tag="POA77_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDI6366522.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGT"
BASE COUNT         4543 a         5424 c         5460 g         4155 t
ORIGIN      
        1 cacctgacgc gaattgcaca ggttcacaca atctatttaa cgcttgcaga atacccctct
       61 ttacgatcga taaaaagtcc ttttcttatt tattttgaag cgtaaaatgc ggggtgatga
      121 aggagtggtg attttatttt caccgccatt agggcggcat tgccgatatt tttttgcggt
      181 acaaatatgc cgtgttcatg gcctgtacga gataacatga cggatcactt ttatgagcaa
      241 aaaatttgaa ggcagcgtcg cgcctcgcga gcgtattaat atatcgtatg tcccaaaaac
      301 agatggccag acggctgaag tggaacttcc gcttaacatg ctcgttgtcg gcgataccgg
      361 aaatacgcag gaaacatccc cactggatga acgtcaggca gtttccgtta acaagcacaa
      421 tttcggtgca gtgatggctg aagcagccat tggcctcaat tttactgtcc ccgccacgct
      481 gaagggaagc acaacagacg atgaactgaa tgtggcgctg aatatcaaat cactggatga
      541 tttttcacca gatagcgtgg cccggcaggt tccggaagtg aataaattgc tggaactgcg
      601 tgaagcactg actgcactga aggggccgat gggtaacctt ccggcattcc gtacccagct
      661 ccaggcattg ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa tcggccaggt
      721 gagcaataaa taaccaccac tatttcatta aacagggtta tccaggcatg acggcacaca
      781 aaaatatctc cgggacatac cacctctctg ttgcagacat tctgcaggtg gtttaccagg
      841 tatgtttcag cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg attacaacac
      901 tggatcgccg tatcagtgat ctattggatg agattatcca tttttgtgag tttcagcaga
      961 gcgcgtcgca ctggcagcgc gtacttcact gacggcgaaa agccggtttc agaaacacag
     1021 ctaaaacaga aggattttca gtatgtcttt acaggaagag gaactcgttt ccagtcatgc
     1081 cgggcagcct gagcaggcat cgtcgctgct tgatcaaatc atggcacaga cccgtattca
     1141 gcccggttcg gaaggttatg acgttgcccg ccagggtgtg acagccttta ttgccagcat
     1201 cctgcaatcc actgcatctg ccgaaccggt caataaactg gccgtggaca gcatgattgc
     1261 agatatagat gaacgcatca gccgccagat ggatgtcatc atccatgcgc ctgcctttca
     1321 gcaggtcgag tcgttctggc gctcactgaa aaccatggtg gatcgcgttg atttccgtga
     1381 aaacatcaag gtcaacgtgc ttcatgtcac caagcaggaa cttctggaag actttgaatt
     1441 tgccccagaa attattcagt ccggatttta taaacacgtt tattcgtccg gattcggaca
     1501 gtttggtggt gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa ataccgcacc
     1561 tgacatgaaa ctgctgcaat acgtcagtgc tgtgggcgca atggcgcacg caccgttcct
     1621 gtcctccgtt tccccggagt ttatggggct gaactcgtgg accgaactgc ctaatatcaa
     1681 agatctttat gccatctttg aaggtccggc ctacaccaaa tggcgcgccc tgcgtgactc
     1741 ggaagattcg cgctatttag ggcttacggc accccgcttc ctgcttcgcc agccttattc
     1801 accaacagat aaccctgtta agaactttaa ctatcacgaa gatgtcagcc ggaatcacga
     1861 agattatctg tggggtaata cggcctggat gctggcgtgc aatgtcgcag acagttttgc
     1921 caaataccgc tggtgtccaa atatcatcgg tccgcaaagc ggcggtgcag tgaaagatct
     1981 gccggtgcat ctgttcgaaa cgatgggtca gattcaggcc aaaatcccaa ccgaggtgct
     2041 ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt tttatcaccc ttaccatgcg
     2101 taaagactct gataatgcag cctttttctc tgcaaactcg gtacaaaaac cgaagcactt
     2161 tccgggtaaa gacgcagaaa ccaactataa gttgggtacg cagcttcctt atctcttcat
     2221 catcaatcgg ttagcgcact acatcaaggt gttgcagcgt gagcaactgg ggtcatggaa
     2281 agaaaggagc gacttagaga gggagcttaa tacctggatc cgtcagtatg ttgccgacca
     2341 ggaaaacccg cctgcggacg tgcgcagccg caagcctctg cgtgctgcca gagttgaggt
     2401 tatggatgtg gaaggcgaac ccggctggta ccaggtcgcg ctgagcgtgc ggccccattt
     2461 taaatttatg ggggcaaact ttgagctttc cctggttggc cggttagaca gggagtaaca
     2521 cgccatgata ccggatcggg aaagaaccac aggttctctt tttgagcgta tggaagcctc
     2581 accggccaga aactggcagg gaagcagtat tcatgcgctc aggcagtcca ttcgccagaa
     2641 cctgcgaaac attcttaata cccggtccgg tagttgccgg ggtgcgcctg aactgggcat
     2701 tgatgaaccg gaaggggctg aaaacttccg cgaatcaatg agtctggcca tcgcgcaatg
     2761 tatagaacgc tatgaacccc ggatctcaca cgctgaagtg caggctgtgg tttcatccgc
     2821 caccagcccg ctggatatga cgtttcatat tacagcctgg gtgacattta acgaaacaca
     2881 tgaagtgctt gaatttgata tggcaccgaa tggcagccag cattatcggg tggactgaca
     2941 gatggaattt gaagaacgct atttccggga agaactcgat tacctgcgcc agctcagcaa
     3001 gctgctggca acggaaaaac cccatcttgc ccgcttcctg gccgaaaaag atgcggatcc
     3061 ggatattgaa cgcctgctgg aaggcgtggc ttttcttacc ggcaatctcc gccagaaaat
     3121 tgaggatgaa ttcccagaac tgacgcacgg gcttattaag atgctctggc ctaattacct
     3181 gcgtccggtt ccggcaatga cccttattga atatacgccg gatatggata agtcttctgt
     3241 gccggtgtta atcccccgta atgagcagtt tacaaccaac gccggggaaa tcagagtcga
     3301 tgaagtgctg ccctccgatg ctaaaaagga ggagccgcct ccctgtacct tcaccctctg
     3361 ccgggatatc tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca gtacgacccg
     3421 taatggtgtt atcaacatca ccttttcggt cgcaccggga acggacttcc gcacgctgga
     3481 tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac tatacccgta accagcttta
     3541 tttatggttc tgtgaatact tgcagggtgc cgacctgact gtgggtgaac agcatattcg
     3601 cctgcctgag tttatgctaa aagctgtcgg ttttgagccg caggatgcca tgctgccctg
     3661 gccgaaaaac gtccacagcg gctaccggat ccttcaggag tatttctgtt accccgatgc
     3721 gtttctcttt tttgatcttt gtggttgtcc ggctttgcct gacggattgt aggcggaatt
     3781 ctttaccctg caactgcgtt tttcgcgccc tttgcccgtg gacatccggc tgcgccgcga
     3841 ttccctgcgc ctgtattgcg cacctgccat taatttattt atccaccatg cagaagccat
     3901 cacgctggac aaccggcggg cagactatcc gctggttccc agccgccatt acccacaaca
     3961 ttacgatgta ttttccgtta acagtgtggt gagccaggtc caggatatgt tcaggaaaaa
     4021 agatctgggg cgtcctgttt cgacgcaggc cgcgcgccag tggccagcct ttgaaagttt
     4081 cagccatcag atggaataca gccggaagcg ggaagtggtg tactggcatc accggaccaa
     4141 aacatccctg ttccatcgcg gctttgatca tacccttgcc tttatacatg ctgatggcag
     4201 ttatccgtca gacgaatctc tgctcagtaa tgaagtggtt tcggtatcgc tgacctgtac
     4261 caaccgtgag cttccgtcac aaattcgttc cggcgatatc accggcacaa ccggtaaaaa
     4321 tgcagctgtt gcttcatttc gcaacattac ccgcccgacg caaccactct ggccggtcat
     4381 tgatggcagc ctgcactggt ccctactctc cgccatgaac ctgaattatc tgtcattact
     4441 ggatacggac gcgctgaagc aggtcatcgc caactttgat cgccacgcaa tccatcatcc
     4501 gcagacggca cggctgtcac aacaaaagct ggatgccatt gagcgtctgg agacccgccc
     4561 cgttgatcgc ctgtttacgg gtattcccgt ccggggactg gcctccacgc tgtatctgca
     4621 cccggagccg tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc tttcgcattt
     4681 tctgtcgctg tacgccagcg ttaactcatt ccacatgctg accgttgtga acacagaaag
     4741 ccaggagaca tggaaatgga cggaaagaat cgggcagcat cctcttatct gagtccggga
     4801 aatcccccgg cagataaaga gcagaatgat ccgctggcac aggttttcca taatgcctgc
     4861 tcttacaatt tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg tgaaaagggg
     4921 acgccagaat tatccctgcg tgacgatccg gcacaggaaa ccctgcgttt cagcgccgat
     4981 gccagccttg ccttcccgtg cagtgatatc agtgcgctga aacgggatac atcaggggca
     5041 ttcaggatga ccaccacctt tatggggtta caggggagcc agtcgcctct ccccggttat
     5101 tacctcgatc acctggcctg gaaagcggta catgaacaaa gtccggtagg tgattttctg
     5161 gatatgttca gccaccgcct gacccagttt gtctggcata tctggcgtaa gtaccgctat
     5221 cacatcagtt ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta ctcccttgtc
     5281 gggctgggcc accgccagct tcgcgataag ctggcgatta atcacagcaa aatgctggcg
     5341 tattccggta tcctggcaaa tccgggacga tccccggaaa taatctgtgg cctggtgtcc
     5401 cactgctttg atttgtcaga agtgacgctg caaaactggc aacgtcgcaa ggtggatatt
     5461 gaaccggatc agcaaaatag tctggggagc tattctctta aaaatggcga aaaactggcg
     5521 ggaagatcgg tactgggcaa ctttgttctg ggtacacgag tgcccgatct cagcggaaaa
     5581 ttccagctca gtattaccag cctgacccga aaacagttcc tctcttttct gccgtccggc
     5641 gaaaacttcc tgccactgac gatgttcgtg tccttcatcc tgcgcgatca gcttgcctgg
     5701 gacttacatc tggggctggc gccggagcag gtgggcgcaa tgcgcctggg cgataacaaa
     5761 agtgcgctgc tgggctggac cagcttcctc ggcacaccgg aagaacgacc ctcagtcacc
     5821 atcagggtac ggtcgtaaat tatgataatc acggagcaga atatgccgga agagaaacta
     5881 cagacgcttt cattgcaggt cattaacggc agtgagctgg aaagcggacg ggcggcgcgc
     5941 tgtctgttca cacagcaggg aaatgtcggc catggccccg aatgccactg gtcggtacag
     6001 gatcgtcagc agagcattcc ggcccaggct tttaccgtta tcctgcacga tggcacattt
     6061 tgtctgcgcc cgcagacggc acaactgtgg ctgaatcagg caaaagtcac agcaacatca
     6121 gacctaatac agttgcgcca gggcgatgag atccagatcg gacggctgat ggtgagggtt
     6181 catctgaacc ggggagatat tccccattac gatgaggaaa tggccactcc cgaaaccatc
     6241 gttaccaatc gcgatatgct cacggatacc ctgctatcaa cggagggtac gccacactat
     6301 ccgggaatga ctcaccggca ccaacttgca gacaccgtgg taaacggttt ttctgccgat
     6361 ccactccagg cacttcagtc cgaaagcctg attaccacgg gcgatccgct ttcaggcatt
     6421 gcggctgtcc ggccatcggc accgctgtcc gatccggcaa gtaatggggg gatcaatact
     6481 ccgtttatgg atctgccgcc catttatgcc agccccggcg atcgtaatga cgacgtctct
     6541 gcggcagaaa tggcgcaacg ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc
     6601 tcgcttacca tgagcaattc cgacgatgcg gatgattttc tggaggaggc cggacgaacg
     6661 ttacaggccg caataaaagg tctgctcgat ttgcagcagc agcgtaacag cctctcagac
     6721 aaacatttgc gcccgctgga agataacccg ctgcgcctga acatggatta cgccaccgcg
     6781 ctcgacgtaa tgtttgccga aggtaaaagc ccggtacatt tggcggctcc cgccgccgtc
     6841 agtgaaagcc tgcgcaatgt ccgccaccac gaagaagcta accgggcagc gattgtggag
     6901 tcgcttcgtg tcctgctgga tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag
     6961 taccgccgca gccatgaact gcgccagccg ctggatgatg ccggagcatg gcaaatgtac
     7021 agccattatt acgaagaact ggcctccgat cgccagcagg ggtttgagat gctgtttaac
     7081 gaggtctacg cccaggtcta tgaccgggtg cttcgtgaaa aacagcggga gccggaagca
     7141 tgaacaggaa ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa
     7201 cggcgaaaaa aatcagtgag gtgatcaaaa acccggatat ccaggtcggg agcctgaagg
     7261 agcaaccttc ggaaataacc gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg
     7321 aaggtgaatc agccgccgtg gatgtgcaac tggtgtacct gactgacgac tcaaaattgc
     7381 tggcagccga ctatgaccag atagccagta cgccgctacc ggatgtgctg ggtaaaaact
     7441 atatcgacca ccaggatttc aacctgttgc cggacaccat caaaaccctg ccgccggtaa
     7501 agctggatga gaaaacgcag tttattggtg tcgtggccta tttctccgat gaccaggcca
     7561 ctgaatggaa acaaatcgag acggtggaag gaaccggcca ccattaccgc ctgctggtgc
     7621 atgtacgtca aagcagcatc gaaatgaaaa aagaggatga ataattatgg caaccacccg
     7681 caacaaggtg atgtggcagg aagggatgct gatgcgtcca caccatttcc agcagcagca
     7741 gcgttacaac gactacctgg ataaccagcg tttccgggcc atgaatgatt tatcctgggg
     7801 atttaccgaa ctcactctca acaatgaact gctggcacag ggtaagatca tgattgacag
     7861 cgcgtcaggc acactgcccg acggcaccgt cttttctatc cccgaccagg acgcactgcc
     7921 cgatccgctg cacccgcaac attttccgga cgagagaagc cgcaatatct acctcgctct
     7981 gccggtcgcc agtgatgtga gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg
     8041 gctgaattat gccgatgtcc gggatttgca ttcagaagaa ggtgacacgc gaacactgac
     8101 gctgggacaa ctgacgccgc gcattatgag cggtgcagaa gatatgagcg cctatattac
     8161 gctcccgctt tgccgtatca gcgatcgcca tgccgacggc tccctgacgc tggatgacga
     8221 ttttatcccc tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt atctcaaaga
     8281 ggtacagggg gccattggcg gacgggcaag cgatctggca aaccgcattg gctctccggc
     8341 gcagagcggc atcgcggatg tggcggaatt tatgatgttg cagttactta accgtaacca
     8401 gacccggttt acccatcacg cccgtcgatc ccagctccac ccggaagatt tctaccttga
     8461 tcttgccggg ttgctgggtg aactgatgac ctttaccgag ccgtcgcgcc tgccctgccc
     8521 gcttgatgtg tatgatcatc atgacctgac caaaacattt aaaacgctgt taccggaagt
     8581 caaacgggcg ctgcataccg tactgtcgcc aagagccgtc aatctgccgc tgcatctgcg
     8641 tgacggtatc tggcaggccg atatccatga cacagaactg ctgcaatctg ccacctttgt
     8701 gctggccgtg gcggcaaaca tgccggtcga tcagatccag cgccagttta tccagcagtc
     8761 gaaaatttcc tcgccggaaa aaatccgcaa tatggtcagt gtgcagatcc ccggcattcc
     8821 attgcgcgca ctgatggtgg ctccccgcca gcttccttac cattccgggt tcagctattt
     8881 cgaactcgac aagagcggac aggcctggac agaaatggct gccgccgggg ccgttgcact
     8941 gcatgtatcc ggcagtttcc cggatctgaa catgcaactg tgggcgataa gagggtaaga
     9001 cgatggatga ggggtcactg tctctgccac catttaccgg atacgacgaa aaaagccagc
     9061 gcaactacca tctcgcgctt cgtggcaaca gcctgaatcc gatgatcgac gcggccacgc
     9121 cgctactcgg tatggtaatg cgcctttcaa ccatgaacag ccagacgatg ccggagcatc
     9181 tttttgctca ggtggtgacg gatgttcagg ccgtggagca actgcttcag gagcagggct
     9241 acgaaccggg cgtgattatc tcgtttcgct acatcctctg cacgtttatt gatgaagcgg
     9301 cgctcggtaa cggctggtca aacaagaacg agtggatcaa gcagtcattg ctggtccatt
     9361 tccacaacga agcctggggt ggagagaaag ttttcatcct gctggagcgc ctgatccgtg
     9421 aacccaaacg ttaccaggat ctgctggaat ttctctggat ctgtttttct ctgggatttc
     9481 gcgggcgcta caaagtggca gcacaggatc agggggaatt tgagcagatc taccgccgtc
     9541 tgtaccacgt tctgcataaa ctgcgcggcg atgccccttt cccgctgtta caccaggaca
     9601 aaaaaaccca gggcgggcgt tatcagctca tcagtcgcct gactgttaaa catatttttt
     9661 gtggtggcgt tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg ctggacagcc
     9721 agacgcagga catcctgcac cagctaaaca aattacttcg ataaggacgt ttatgatcca
     9781 gattgatctt cccacgctgg taaaacggct gaacctgttc tcccgccagg cgctggagat
     9841 ggccgcctct gaatgtatga gtcagcaggc agcggaaatt accgtcagcc atgtgctcat
     9901 tcagatgctc gccatgccac gcagtgacct gcgggttatt acccgccagg gcgatattgg
     9961 catggaagag ttgcgccagg cgctgacggt agagaactac acaaccgccc gttctgcgga
    10021 cagctacccg gcgttttccc cgatgctggt tgagtggctt aaagagggct ggctgctggc
    10081 gtcggctgag atgcagcaca gcgaactgcg cggcggcgtg ttgctgctgg ccctgctgca
    10141 ttcgccgctg cgttatatac cgcctgctgc cgcccggctg ttgaccggca ttaaccgtga
    10201 ccgtctgcaa caggactttg tgcagtggac acaggagtcg gcggaatcag tcgtgccgga
    10261 tgcagacggt aaaggcgcag gcacaatgac ggacgcctct gacaccctgc ttgcccgcta
    10321 tgccaaaaac atgaccgcag acgcccgtaa cggcaggctt gacccggtac tgtgccgcga
    10381 ccacgaaatc gacctgatga tcgacattct ctgccgccgc cgtaaaaaca acccggtggt
    10441 ggtgggcgaa gcgggcgtgg gcaaaagcgc actgattgaa gggctggcgc tgcgcatcgt
    10501 ggcaggccag gtgccggaca agctgaaaaa caccgatatc atgacccttg acttgggcgc
    10561 attgcaggcc ggggcgtcgg tgaagggtga attcgaaaaa cgtttcaaag ggctgatggc
    10621 ggaggtcatt tcctccccgg tgccggtcat tctgtttatc gacgaagcac ataccctgat
    10681 tggcgcgggc aaccagcagg gcgggctgga tatctccaac ctgctcaaac cggcgctggc
    10741 gcgcggcgag ctgaaaacca tcgccgccac cacctggagc gagtacaaaa aatacttcga
    10801 aaaagatgcc gccctgtcgc gccgcttcca gttggtgaag gtcagcgaac ccaacgctgc
    10861 cgaagccacc attattctgc gcggtctgtc ggcggtctat gaacagtctc acggcgtgct
    10921 gattgatgat gacgccttgc aggccgctgc gacattaagc gagcgttatc tctccgggcg
    10981 tcagttaccg gacaaagcga ttgatgtgct ggataccgcc tgcgcccgtg tggccatcaa
    11041 cctgtcgtcg ccgccgaagc aaatctcggc gctgaccact ctgagccacc agcaggaggc
    11101 ggaaattcgc cagcttgagc gcgagcttcg catcggactg cgtaccgaca catcacggat
    11161 gaccgaggtg ctggtgcagt atgatgaaac gctgacggcg ctggatgaac tggaagcggc
    11221 ctggcaccag cagcagacgc tggtccggga gattattgcg ctgcgccagc agttactggg
    11281 cgtggcagag gacgatgcgg cgccgttgcc ggacgcagat accgtggagg atacgcagcc
    11341 agagtcagaa caggataata ccggtgctaa actggctgat gaagctggca gcgaacagcc
    11401 ggaagagacc gcagaaacag tttccccggt gcagcgactg gcacagctca ctgccgaact
    11461 ggacgccctg cataacgacc ggttgctggt ctccccgcac gtcgataaaa aacagattgc
    11521 ggcggtgatt gccgaatgga ccggcgtacc gcttaaccgc ctgtcacaga atgagatgtc
    11581 ggtcatcacc gacctgccgg tatggctggg tgacaccatc aaaggccagg acctggcgat
    11641 tgccagcctg cataaacacc tgctgactgc acgcgccgac ctgcgtcgtc cgggacgccc
    11701 actcggcgcg tttctgctgg ccggtcccag cggcgtgggt aaaaccgaaa ccgtcctgca
    11761 actggcagaa ctgctctacg gcggtcgcca gtacctgacc accatcaata tgtccgagtt
    11821 ccaggaaaaa cacaccgtct cgcggctgat tggctcccct ccgggctatg tcggctatgg
    11881 cgaaggcggc gtactgaccg aagcgattcg ccagaaaccg tactcggtgg tgctgcttga
    11941 tgaagtggaa aaagcgcacc cggatgtcct caacctgttc taccaggcgt tcgacaaggg
    12001 cgagatggca gacggcgaag gccgactgat tgactgtaag aatatcgttt tcttcctcac
    12061 ctccaacctt ggttaccagg tgattgttga acacgcggat gacccggaaa ccatgcagga
    12121 agtgctgtat ccggtgctgg cggacttctt taaaccagcc ctgctggcgc gtatggaagt
    12181 ggtgccgtac ctgcctctgt cgaaagagac gctcaccacc attatcgacg ggaaactggc
    12241 ccgcctggat aacgtgctgc gcagccgctt tgatgcggac gtgattattg agtcggaagt
    12301 gacggacgag atcatgagcc gcgtcacccg cgcggaaaac ggcgcaagga tgctggagtc
    12361 cgtcattgac ggcgacatgc tgcccccgct ctcgctgctg ctgttgcaga aaatggcggc
    12421 caacacggcg attgcccgca tccgcctgtc ggcggcagac ggcgcgttca cggcagacgt
    12481 ggaagatgct ctggacgacg agtctgtcac agaggatgaa acggatttat gagcaggttg
    12541 cgcgttttcc ggcagacagg atggtttatc gcaggcctgg tggcgggctt acccgccagt
    12601 gccgcgcccg cagaaacatc gtcactggcc ggtgtggagg tagccgtcgc cactgcgacc
    12661 ccgccggatg cgactgccac gttacatgcc atgcagtcct gtcggcagga gacggcggcg
    12721 ctggagcggc ttgactgtta cgaccgcctg ctggcaccac tgtccccgtc aggatttaac
    12781 ggggcactgg tcaaagccag ctttgtgggc gaagcctgga cccgcgctac ggagcaggaa
    12841 aaacgccgcc agggcaacac cacagagctg ctggtgacgc aggtgccggg ggaacgcccg
    12901 acggtggtga taaccacccc ggctatcggc catgttccgc cgcgcccggt gctgatgttc
    12961 agttgcgtgg acaatatcac ccggatgcag gtggcgctga tgcaccctct ggatgttcat
    13021 gatatcgccg tcaccctgaa tgccgacagc cgggcattgc gcagccactg gtttgtgcgc
    13081 gagaacggca ctctgctgga atccagccgg gggctgtccg ggattgatga aattaagcaa
    13141 ctgttcggcg caaaaacgct gatggtggat accggcgcag acaacgccgc cggaaagctg
    13201 acctttaaca ttgacgggct ggcaagggcg attgctcccc tgcgcgatgc ctgccactgg
    13261 gcgggagaat aaaaatatgg caatggactt acgcgatccg aatgtctggg tatcgcacct
    13321 gctggaaaac ctgccggaag agaaactggc atcggcgctg aaggacgaca acccggactg
    13381 ggagtatatc gacggtggga ttgtcaaact gggatcgctg gcgcacagcc agctggatat
    13441 ccctgaactc cagcaccggg gactggtcct tctggcttcg gagagtaagg attttcgcct
    13501 gctggcgcat ctgctgagaa ccctgcaaca tgccggtgat ccgctcctgg ccttgcgcct
    13561 gctggcgctg tatgtggagc attactgggc ggtagccgcg ccgcagaata tggcacataa
    13621 aaaacgcttt gccagccagg ttattaaacg cttcgaaaca ggtattgacg ctttttcaca
    13681 gaacgccgcc acagcgcagc gcgatgcgct gctaggtgaa ctggcgaaac tggcgcaatg
    13741 ctggcagtca cataatgccc cggaactagc acaggctact gatgatcttt ttgcccttta
    13801 ccagcgtgcg tttaatcgtg cggcaccggc tccagccccc actccggcgg cctccagtag
    13861 ttcaccacag acgatcgcca cgtctgaaag cggcgtgacg cataccagta ctccgactcc
    13921 acagatcgcc atcgacagtc acgacgacaa aacctggcgc gacacactgt taaaagtggc
    13981 ggccatttta tgcgagcgcc aaccggaatc gccgcagggc tatcgcctgc gtcgccatgc
    14041 cctgtggcta accatcacca gtacgcccca ggcggaaagc gatagacgta ctccactggc
    14101 tgcggtctct gccgatatgg tggcggatta ccagtcccgg cttgccagcg cggatatgga
    14161 gctgtggcag caggtggaaa aaagcgtact gctggcaccc tactggctgg acggtcacta
    14221 catttctgca cagacggcgc accgtcttgg ttataccgct gtagccgatg ccatccgcga
    14281 tgaagtcgtt tcctttcttg gccgtctgcc cgcgctcgct gatcttctgt ttaacgacca
    14341 cacaccgttt gtttctgaaa aaacgaagca atggcttgca aatcagccag gtaaccagtc
    14401 cgcgccagtc atacaggtga gtaatgaaga tattcaggct gcgcggttgt gttttgatga
    14461 gcaggggctg gaagccgcct tacggtatct ggatacgtta cccgccggtg aaccccgcga
    14521 ccagttccac cggcagtatt ttggcgcgca gcttatggaa gaggcggggc tggtacagct
    14581 tgcgcaacag cagtacagga tgttgttcag gatgggattg cagatgatgg tggcagactg
    14641 ggagccgtcc ctgcttgagc agctagaaca aaaattcacg gcagaacaat aaaagagaac
    14701 aggagttttt gtgttcagat taccaacacc ccgactattc agcggactca aatcagccct
    14761 gcgcccggcg atgccccgat ttaaagtctc tgcattctgg ctgctgatac tggcgtggat
    14821 tttcctgctg gtttggatct ggtggaaagg cccgacgtgg acgctgtatg aagagcagtg
    14881 gctcaaacca ctggcgaacc gctggctggc aacggcggcg tgggggatta ttgccctggt
    14941 gtggcttacc gtccgggtga tgaagcgcct gcaacagctt gaaaaaatgc aaaagcaaca
    15001 gcgcgaggaa gccgttgatc cgctcagcgt ggaactgaac gcccagcagc gttatcttga
    15061 ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt ggcagttgcc
    15121 gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgc gcgaggggtt
    15181 tccgtccgac attatttatg ccccggaggg cgcacgcggc gcagaacaac gcctgtacct
    15241 cacgccccat gtcggtaaac aggcggtgat ctttgatatc gacggcacac tctgcgctcc
    15301 cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct ggctgaaaga
    15361 aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac ccgatctgct
    15421 gaccgcagac aaacgccgcc gcgagcatct gttacagacg ctgcgcagcc gcttacagga
    15481 tatacgccag catcttcact gccagttacc ggtttacgtg gtactgaccc ggcttgattt
    15541 attacagggt tttgccgccc tgttccagtc actcaacaga caggatcgcg atgcgattct
    15601 gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac tggcgaacag agttgaatgc
    15661 tttctggcag acatgggtgg atcgaatgaa tctggcgttg ccggatctga tggtcgctca
    15721 gactcacacc cgcgcgtctt tattcagttt ttcccgccag atgcagggaa gccgtgaacc
    15781 gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat atgaacgtga tgctgcgtgg
    15841 tgtctatctc acctcttcgc ttcagcgtgg acagatggat gatatattca cccagtctgc
    15901 cgcccgccag taccggctgg gcaataaccc actggcgtcc tggcccctgg tggacaccgc
    15961 gccttatttc acccgcagcc tgttcccgca ggcattactc gcagagccta atctggcaac
    16021 agagagccgc gcctggctga tacgttcccg tcgccgcctg acggttttct ctgccacagg
    16081 cggcgtggca gcactgctgc tcatcaccgg ctggcatcac tattacaacg gtaactatca
    16141 gtccggcatc accgtgctta agcaggccaa agcctttatg gacgtgccgc ctccgcaggg
    16201 ggaagatgac tttggcaacc tgcaactgcc gctcctgaac ccggtacgcg atgccacact
    16261 ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt accagggacg
    16321 acgtatcggg ccttatgtgg aacagaccta tctgcaactg ctggagcaac gttacctgcc
    16381 ctcgctgttt aacgggctgg tcaaagcgat gaacgccgcg ccgccggaga gtgaagaaaa
    16441 actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa agcggacgta acaatcaggt
    16501 ggtgaagcag tatatggcaa aacgctggag cgaaaaattc cacggccagc gcgatatcca
    16561 ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc atgcagagcg
    16621 tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc ccgtggtatc
    16681 agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga gcctgaaaac
    16741 gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag ggccaacctt
    16801 tgaccaggtg tttacatctg ccgatgacaa caaactggtt gttccacagt ttcttacccg
    16861 ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac tgacggcgat
    16921 ggattcctgg gtacttaacc tcacccgcaa tgtgaaatac agtgacgccg accgcgcgga
    16981 aatccagcgc cagttgaccg agcagtatat cagcgactac accgccacct ggcgggccgg
    17041 gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg gggcgctgga
    17101 gcaggttatc agcggcgacc agcctttgca gcgggcgctg accgtgctgc gtgacaacac
    17161 acagccaggc gtcttttctg aaaaactctc tgccaaagaa cgggaggaag ccctggcaga
    17221 gccggattac cagttactca cccgcctcgg gcatgaattc gccccggaaa acagtaccct
    17281 ggcagtacag aaagacaaag aaagcacgat gcaggccgtg tatcagcaac tcaccgagtt
    17341 gcaccgctac ctgctggcaa tccagaacgc gcctgtacca gggaaatcgg cgctgaaagc
    17401 cgtgcagtta cggcttgatc agaacagcag cgatccgata ttcgccaccc gccagatggc
    17461 aaaaacgctg cctgctccgc tcaaccgctg ggttggcagg ctggctgacc aggcctggca
    17521 tgtggtgatg gtggaggctg ttcattatat ggaagtggac tggcgcgaca gcgtggtgaa
    17581 accgtttaac gagcaactgg caaataacta tccgtttaat ccgcgttctg cacaggatgc
    17641 ctcactggat gccttcgaac gcttctttaa accggatggc atactggata ccttctacca
    17701 gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg ataacaacgt
    17761 cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc gtgacatctt
    17821 cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat cgctttcagg
    17881 caataaacgc cgcagtgtac tgaaccttga cggtcagtta gtcgattaca gccagggccg
    17941 taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg aaagtaagct
    18001 gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg ggccgtgggc
    18061 gcagttccgc ctgttcgggg ccggacaact gaccggagta caggatggca actttaccgt
    18121 gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca cggaagataa
    18181 cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt actgaggagg
    18241 cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac ccgcgcgggc
    18301 tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc cagccacccg tcacagcctg
    18361 agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc aacggtgtgg
    18421 atttacacac cgccacctac tatacgcttg cccggacacg gacacaggga ctggcgggat
    18481 tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat aagttctggc
    18541 cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga accggcaata
    18601 ttctgcgtca gcaaatctcc tttgcggaat ccgacctacc actgatctac cgcacagagc
    18661 gggcattgca gcttatctgc gataagctcc agcaggtgga actgaagcgc gttccgcgcg
    18721 tggagaatct gctctatttt atgcagaaca cgcgtaaacg gcttgaaccc cagctgaaga
    18781 gtaacactga gaacgccgca cagaccacgg tcagaacgct gatttatgcc ccggaaacac
    18841 aggcatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctgcct gagatgaaag
    18901 tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag caaggcagta
    18961 cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca gcattgtggt
    19021 ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc gctcagggcg
    19081 cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg ctgcaacaac
    19141 ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg cgtgttgccg
    19201 acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg aatgaggcac
    19261 tcaaaacccg cgcacagagt agcccgcagt tgcgtggctg gttgcagacc cgccaggact
    19321 tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactaacc ctttcctata
    19381 tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaacaccc gttgagtctc
    19441 tgttgacgca gtaccatgat gcccgtgcgc agaagcagaa tacagatgcg ctggaaaaac
    19501 aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaag aataacgtca
    19561 tgccagaggc ggcaaccggt ac
//
LOCUS       DAMZII010000090        19514 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZII010000090 DAMZII010000000
VERSION     DAMZII010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.090"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(71..283)
                     /locus_tag="POA77_003136"
     CDS             complement(71..283)
                     /locus_tag="POA77_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366523.1"
                     /translation="MSETKQAYLMKFRKCSSFDTLEKVFERLCEKNAGIASLEISGAY
                     DHRKAELTMKKLYDKVPASVWTLVRQ"
     gene            498..1679
                     /locus_tag="POA77_003137"
     CDS             498..1679
                     /locus_tag="POA77_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6366524.1"
                     /translation="MNSKPVTRQFEDSDLHQELVTFEVPNNDLKELIFYFSHMKYNTA
                     KTYLQWLRSWNEWYQANAGKEGNEAWPASSLPVTEPPLLAYLDYLQGSLSHSSIKGCL
                     HALNSIHRKALDRPGIITSKVKSILASLEQAEAREQKVTRQATPFLVSDLKALIKAHG
                     TTQSVRKLRDLCIIWTGFETLLRSAELRRIRMQDLVLNEQTGSFTLTVYRTKSTVSTL
                     LTYHLTPHLTATLIRLMDMVKRDQQSHPKDYLFQAVNYQDSGYMPPGWGLRSKGNEIN
                     TLLKNHNMPYRPTRPPIGKNGKPIIVDDEGMLSKNTLLRAFEAFWDELHPQEAGTRCW
                     TGHSVRVGGAIELANAGYTHLQIMEMGNWSNPEMVSRYIRNIDAGKKAMTKFMREALD
                     E"
     gene            complement(1704..2048)
                     /locus_tag="POA77_003138"
     CDS             complement(1704..2048)
                     /locus_tag="POA77_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366525.1"
                     /translation="MELGIMVCIVLLLSLAAVWISNKTVGLLEIHYFKRPLSMEYAAW
                     LRVMCAGLLFIFIQMVPAFTFLYTLKLVALLNLGVQTMKLNGVYKFKRTGMMPPKDAP
                     HDLGRFNPFRKK"
     gene            complement(1984..2892)
                     /locus_tag="POA77_003139"
     CDS             complement(1984..2892)
                     /locus_tag="POA77_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366526.1"
                     /translation="MQWISTTSQKLDERLYRVCVWVKKYHEDAINRVVLTVDLSKARH
                     DPIEDQVRAELLCGHYFLMRKENNAKSAPADQNFEFLPDGRNLSWQTSTPGLQHLLLN
                     SNVPESLRPLADYIQMRLAKLTMVPMSGTIMKAALEDSISWVKVDLRYYWQYELVNSH
                     LGPVQVTHKALVRFGRLAKHDENASAIRMLRQRLSSPFIQEFEMPGEELKRKQKIMNT
                     VDVKMLFHTHYPGQKLLLARYANGWVLVDCFLFHHIKPKKKAKNKTKAGKSETQNIKS
                     TGSEDHGTGHYGVHRVVTLTGSGVDQ"
     gene            complement(2903..3862)
                     /locus_tag="POA77_003140"
     CDS             complement(2903..3862)
                     /locus_tag="POA77_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDI6366527.1"
                     /translation="MASEDLLFYRDRILERIEKLRSFLNDHQPVMAELMTVGTVTRHE
                     ERLKEVNPIEVSTISDASVDEIIRVIQDFCIDDIDVLSRNSTKVTTKYPGLIIVPEGA
                     EQLSGIICDINDAKSDFASAMKRVNSEKHIRFKEVHRKLPGLVTAHSTRKILFVEEQL
                     KKVTFAWRLNRNQVKTNASDLIAMLDKRRLVAVKSPVTTDLTVVANIDRAKALLERKV
                     LRDGEGYRLCRTNTFPVPIAHLFTYRPEGKERGSSKYAETDYKVVKASLPIFGVGQKP
                     TIKRLQDWVPAQDEALSNGRRSNHSYTELVPGADLGIFIMRKV"
     gene            complement(3951..4226)
                     /locus_tag="POA77_003141"
     CDS             complement(3951..4226)
                     /locus_tag="POA77_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366528.1"
                     /translation="MHLLVEGKGVIPDVSVSDLGNVQNIRNELIGLGWWVKFGVKQDW
                     QRINLQRQPVSFGLLNDLNRCFRHDGQQVEPLPLYTTGNPGELRKVL"
     gene            complement(4444..4836)
                     /locus_tag="POA77_003142"
     CDS             complement(4444..4836)
                     /locus_tag="POA77_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366529.1"
                     /translation="MVFWIIISFMFLSAMFMTGHLLNKVVLQPVWLCIWSLPWLGFLL
                     KVRVTEMDKGLVNTLQLGDLPVFRWSRSVVPGTWSVDRINGRYRLNRIGTDGLTVHYP
                     WRLYYQPLFLTPDKQISDINGREHERKF"
     gene            complement(4916..5452)
                     /locus_tag="POA77_003143"
     CDS             complement(4916..5452)
                     /locus_tag="POA77_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366530.1"
                     /translation="MKSKIHSSGTSGTKRVLKTDIALPLLCWVFTSPFSNWTDKFFTG
                     TEVPEGSLPGLEQAPEAIFRFVLNDEGFDVGFDAVGMDLCCFSIPLSTMPTKNLDDEE
                     TLSRLTGDVIHGVLLSLPEYIEMPDRLVYQLTDEVMAFNSHCGNGILHGWTTAQELWR
                     NEILPRTTILMQQTSVIH"
     gene            complement(5526..5783)
                     /locus_tag="POA77_003144"
     CDS             complement(5526..5783)
                     /locus_tag="POA77_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366531.1"
                     /translation="MSNTDSQTVPADALCENSSQVKIQLSRSASRALTTAKEILETRG
                     LVSVSTEDLVQACLEENQPLDLASIYLEHRLKQKGTSICEF"
     gene            complement(5791..6381)
                     /locus_tag="POA77_003145"
     CDS             complement(5791..6381)
                     /locus_tag="POA77_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366532.1"
                     /translation="MMTESFFDWVVYCITGVILASAFLEGKRTRFPAAFFYGSIVLLV
                     VLIVAGALNWWFYRSKFGSVVVIFAATTLLMTRFRLVIYAFRFRTLVADLQSHVAKGF
                     RIILVLPENEDEKTVLLSKLSKVIHSGTLFYTRTALGPYSGDLLHALGQKHRNGEGYL
                     LLCEQQLPARTWLSTVENGQPEKSIAVNFHSIPDME"
     gene            complement(6436..6705)
                     /locus_tag="POA77_003146"
     CDS             complement(6436..6705)
                     /locus_tag="POA77_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366533.1"
                     /translation="MPATTILSNNPVNSVFSNDNSHFTYRHIDFSIELLLSSGTDSQL
                     EYLFLKPAAEVKIDLQRRLASGERYVTTESCDNFSEVDGCMGHDC"
     gene            complement(7118..8074)
                     /locus_tag="POA77_003147"
     CDS             complement(7118..8074)
                     /locus_tag="POA77_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366534.1"
                     /translation="MTNSTDNQNYVRAVLAGIGIDFDETEMFISVSHCQSDEVSFTCS
                     ISASELRESAGHYVDTLNDTQLAGLDADALKKRLVYFLEVFDLVSGQYLDISGKHFAT
                     SRFEYDDVCSEILSNSADSAQPGGYDREEYKRLMEVDGQVLIARFALEKFWDTHFIGL
                     INYVSDEITSGLYEVYRTFSDINMAGYTFSEYSYTRRITDELSLHISLKEDDFEEQLT
                     DCYMDETTLPSGKVVLRRNNESIIGIYEGYASKSYFPMVANVRVLDTDGEVVTELYQG
                     VNVSELAGGRIKIHDRQELISEVFANLREFIAASEDKIFDAA"
     gene            complement(8251..9186)
                     /locus_tag="POA77_003148"
     CDS             complement(8251..9186)
                     /locus_tag="POA77_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366535.1"
                     /translation="MFNESDDKNFVSAMLKCQLGLNISQEDITIYDKENHFEQLSFKA
                     NVALDDLLFYLDLYISELIKHNAPYSETEVLRTKIRYFLKVYEKSGFQNIRIRGYHNA
                     HSTIDIVDIASLILAGSVPESEHDSIDPVLRKEIYQNRMSVEGKVLIARFALKQFFHS
                     DFGDFILEFEKSISKCLNTSLQIIKSVKNSFNRLGQYQYQRRVKDDLTLHLDLNTDEY
                     PACMPDLYIGFKESEGTTGVYRDDEKIIRLYTGVSSGKDVPVMMTVRFTGCDGSVLSE
                     SSHGTFCSVGPTGRVQVCDRVALVQEAVEELRDVV"
     gene            complement(9259..10179)
                     /locus_tag="POA77_003149"
     CDS             complement(9259..10179)
                     /locus_tag="POA77_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366536.1"
                     /translation="MKAIYKIIEAMLVKMGFEGAVIQSVNLETGVIQSLVVKADIPVR
                     TLMPHLRRYLRDCEPLWGHDVDYHPIAQYCSGAALLKAGPVTLETFRFEVSVSTAHLR
                     LVDVEPDCLNEGVNREKLEYVYYRSSNADKDLILGMVEKGHNFINHLHELLRSRLSTA
                     LPLIFAVTEKIAESSVPQVVTSFFSVDHQINAQIVVEPVPVSVADQIISADWNVCSVS
                     GADKFLNHVNRYLSGRVMKRRIYAVIEACDGGSLSLNANLGCFTSPLLVTEYKMVQSH
                     SSSLYRKAINNSLKQVNSYAPEHSDDLLSM"
     gene            complement(10257..11192)
                     /locus_tag="POA77_003150"
     CDS             complement(10257..11192)
                     /locus_tag="POA77_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366537.1"
                     /translation="MKIIEKIINAFLISRKHSVKVSNVICLSGTDGKFTGICCDADVS
                     FDFLYSYAPAYSSTFLDIPFPGFEDQDIADCVKCQLDVVKNKRNRSFLIDHIRFPVSS
                     REGFTLTRGDSYDVTECEYNKERLLHLTRQGRFCEDYLTFKDGLSSFFSFVNFELHEI
                     VKEGIRLALDVLNKITSDQPDRLIKDFKYHDCFGSYNVQIFSKGLPVDILETMIAPDR
                     LLSNLSGSRQIMKNASRYLKGFALSNRVYMKYGWRLADVDTSDYAKVMSDREMHARDD
                     LKAFCSVFFKECFASGKYEYEAYLSERQHAIRNGY"
     gene            complement(11263..12141)
                     /locus_tag="POA77_003151"
     CDS             complement(11263..12141)
                     /locus_tag="POA77_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366538.1"
                     /translation="MKLATDLVNAVLIKNGIVPTSINELPVTSLVKPLTQVHLSLYLK
                     TTHLSQFVPDYVESLKDCPVSYDIGLELQQCINSAELLCEDMESLEFISVWIELYTCN
                     SKQQRFEKSNFSFRTEDIELAKMLSCSHEILISQAACFWLLYFEDFMKYVRNRLCALL
                     RQVLILIAGLRNVYLQRNEDQLIKRVKHGNEMLEVTLKAVALCSIERYLKSYASLRTE
                     AAASGFIKCIQSYISTGWRHGYIVCQYSKNGRSLGKSLPLFIKETSEHNIQKRVNRLI
                     SSTISGRNKDLGLYDF"
     gene            complement(12219..12743)
                     /locus_tag="POA77_003152"
     CDS             complement(12219..12743)
                     /locus_tag="POA77_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366539.1"
                     /translation="MNIENQDLFNTFAAVISSHIVEQPSSCYYLHDNEIDFTILKHSI
                     IDKDKNLLYVIRPSGTCLLRCDKYFFPNYYLTSRGDYKAFKYVHFNLATREAEEITWQ
                     QAFEILSKPGRPPLRGSLGKFDYLKLVIDDLRARGYADFLPAYNLDGLRHFAVKDERP
                     SLVSYIDNVMALCA"
     gene            complement(13018..14250)
                     /locus_tag="POA77_003153"
     CDS             complement(13018..14250)
                     /locus_tag="POA77_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6366540.1"
                     /translation="MTYASQAVKRTPHEVTDHFIKMVHARIAEVSGWRYVFDRIPAFK
                     DACDKAPGQVPCPFSGVGKSKFRFRKKDLFTGCAIHNDFPVNAFCDGIDVLAEYYKLS
                     KTQTCKKILTDFFGMDLYAPLTDADLESERRYKSTVRATETLDSDEVEKRGRKLEVIY
                     HYTGEIKPESPVWVYLRNRGLNRVLSNLPKDLGLNKRLYYMDKSLEKPTIYPGMIAIY
                     RDTRGRPLTIHRTFVELNGDKAHVENPKLMMKPPADMTGGSIQLYDPHFNPGTRTWTL
                     GVAEGIENALSVTEATSTPCWAASSAWCLENVEVPDSLLPPPGVKVIQFYIWADKDLV
                     NTKGTSPGMESAKRLQERMKEFFAKRYPTSELTIKVFEPDFDIPVGKKGVDWNDVLKL
                     TGPDGFPVKWAPECLAQL"
     gene            complement(14365..14586)
                     /locus_tag="POA77_003154"
     CDS             complement(14365..14586)
                     /locus_tag="POA77_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366541.1"
                     /translation="MTQIKTYRVEYEKVGTMHRVRIFGRMGEIVKSELPEERILRDVS
                     IPEGNGEMATSMVDGFIQRLENIGFKTEA"
     gene            complement(14658..16670)
                     /locus_tag="POA77_003155"
     CDS             complement(14658..16670)
                     /locus_tag="POA77_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6366542.1"
                     /translation="MKFDLKSSPRHIQRLTNIASVISGINGIYVIIDNKVSTPYFNTQ
                     NNVCVLPNGDYSDERFVKLIEGFICHEAGHGRYTEHEVYREAFVGELINADGFISIDD
                     DLKADFQNLKQKQKAYARACRLKGLINLFDDVQMEEKTGIDYQEAKKRLAVTYALMVE
                     AGRMTVDISSTPQNPVQFIEMYLLNTLRVNVLQQEGHKETLDPFFDYAKKILAPVTSE
                     VDEIIHQALSCKSTQNCDSLARKTLALLERLRDEAKEKQQEEEQSKDPHDDTDESSGS
                     ENEPDTEPNGDSQGEGDGDKEGQGDDSGDGKAPAEGNGLPDQSGGDEQNAENSNGEPD
                     GESEGDESATPGSDDADSTPSLNDSSNGNSSDGESNFSPEQWDMLAKLLDDFLNSDEE
                     SEDFHEVLAKEISVIAASVSDEVKAEFGASEWDVPDLNIDLNVYNEALNISQTLGADL
                     SVLQQVKMRGQFKTRDRGLSFDINRLIQSPMGVRDVFRSQSESKNRGHVGLVIVRDIS
                     GSMSLEHRYIHAIKTDLALTLAIEAISKMHVANVIYPFVDKDFEVIKTFDENAEEKLS
                     KFSLGCKGNNTPTGSALNAALELLLESQFDRKIVFLITDGYPTESAYTINDVFSVAES
                     NGIEIAGVGIKTDVLMGFNEGTFVNVDDISLLPNEVSKLVHQILSN"
     gene            complement(16748..17875)
                     /locus_tag="POA77_003156"
     CDS             complement(16748..17875)
                     /locus_tag="POA77_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HDI6366543.1"
                     /translation="MTFNTSKLNKVSAKEISENFIKAYPDLQEGAVITKIEISGCQGS
                     RRTDKIELSYGGDDITDQKSVSKSEVRWIDIKALSFKSTITSRISTLCSRLCIRYSNM
                     WILPVERMEEFLEEAQEIEREFQAGIQNVVDNYDTHIQAEKDRSPLMSDLIDQLKLTK
                     DDFVKSFRFNLAHFIPFSPISVEGDETQDFYQEQLITDLADEAMKVYTKISKNDNLRS
                     STIDRLKQMQDKIISFMFIHKEAVVLAEAIKHIMNNLPKGAISSPRDVAVLKQWFYFM
                     SDASMLKRIISGEQKVTDWLDSITRSFNIDSQVTAANSLNTAEDDVFSSANVFREDPV
                     EDLENKAVNAPEPAVGPDEDVSIQASGSNRAFDCELELTGW"
     gene            complement(17946..18215)
                     /locus_tag="POA77_003157"
     CDS             complement(17946..18215)
                     /locus_tag="POA77_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366544.1"
                     /translation="MTDFAHLTFYGFLSANGTETFVIAQDGNQYIGLKQHNNDYSFIC
                     YEVCNKDMEAMIKGLESKGFMYQGSIQSADVENEFPGILLTTLEK"
     gene            complement(18254..19306)
                     /locus_tag="POA77_003158"
     CDS             complement(18254..19306)
                     /locus_tag="POA77_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6366545.1"
                     /translation="MTNAATVLKSDNSVQEEIIGNLLPANVISSKMFANKEQYYLVKA
                     KDLFGIPEMDEDMIVPVFKKFSPYRANLNKDYIPNSRILEQVVKLLVSNDIDLSVCLK
                     GESGSGKTEMAMYISHMLNWPLTIKQINSNIRADELEGERSLINGNTSFVPSELVTAF
                     QEGHLILLDEVDKIDPDTAAKLHMPIERKPWSLAANGGEVVKANKFTRFMGTSNTNMS
                     GGSRKFVSSQRQDAAFIKRFLIVEMERPSEVALTNVLLKRYNNLPMLVIEKFVSVAVA
                     VNNTGTDDSVMDIRQLVAWVGTSMTLKTMSLLDTFKIAFASALPAHATGMVLEAIDLI
                     LGDDKNRTMEYYTAKK"
BASE COUNT         5563 a         4627 c         3895 g         5429 t
ORIGIN      
        1 tactgctgac gttttcataa atctcctttc ctgaaaagtc ggcgataaat tggttaccaa
       61 tatcttcata ttactgacgg accagtgtcc atacgcttgc gggtacttta tcgtagagtt
      121 tcttcattgt cagttcagct tttcgatggt catatgcccc ggaaatttcc agtgatgcta
      181 tacctgcatt tttttcacag agtcgttcga atactttttc cagtgtgtcg aaagaagagc
      241 acttacgaaa tttcattaaa tacgcctgct tagtttcgga catggtcaac cttaatttat
      301 ttcccgcgaa agtacggttt cccaatctta tgtcgttttg tccctagaat ctaatttttt
      361 tcacaaatat ttgatgttta tctccggatc gttgttgtag tcgctctctc ttttttagtt
      421 gctgtgaacc tgttcataag tggatacctc agtgctaaaa tagctgtaaa taaaaacagt
      481 gtattcaggt gaccaccatg aacagcaagc cagtaaccag acagtttgaa gactcagacc
      541 tccatcagga actggtcacg tttgaggtgc ccaataatga ccttaaggag ctcatttttt
      601 acttttctca tatgaaatac aacacggcta aaacttacct tcaatggctg cgttcctgga
      661 acgagtggta ccaagctaat gcaggaaagg agggaaacga agcctggcct gccagttctc
      721 tcccggtgac agaacccccg ttgttggcct atctggacta tcttcagggc tcgctgtcac
      781 acagcagcat taaaggttgt ctgcacgcgc tgaacagtat ccataggaaa gcactcgatc
      841 gccctgggat catcacatcc aaggtcaaga gtattcttgc aagcctcgaa caagctgaag
      901 cccgagagca aaaagtgact cgtcaggcaa cgccattcct agtctccgac ctgaaagctt
      961 tgattaaagc ccacggtacg acacagtcgg tgcgcaaact ccgagacctt tgcattatct
     1021 ggacaggttt tgaaacactg ttgcgctcag cagaacttcg gcgcattcgg atgcaggacc
     1081 ttgtgctgaa tgaacagaca ggcagtttta ccctgacggt gtaccggaca aaatccactg
     1141 tcagtaccct gcttacctat catctgaccc ctcatctcac tgccacccta atcaggctga
     1201 tggatatggt caaaagggac cagcaaagcc atccgaagga ttatctcttc caggcagtta
     1261 actatcagga cagcggatat atgccccctg gatggggtct ccggagcaaa gggaatgaga
     1321 tcaatacgct gctgaaaaat cataatatgc cttaccgacc tacccgtccc ccgatcggga
     1381 aaaatggaaa gcccattatt gtcgatgatg agggtatgct cagtaaaaac acattgctcc
     1441 gggcctttga agcgttttgg gatgaacttc atccgcagga agcgggcacc cgatgctgga
     1501 ctggtcacag cgttagggtt ggcggcgcca tcgaacttgc taatgccggg tacacgcact
     1561 tgcagatcat ggagatgggt aactggagca atccagaaat ggtttcccgc tatatccgta
     1621 acattgacgc aggtaagaaa gcgatgacga agttcatgcg tgaggcgctg gatgagtagc
     1681 gcccctgcgg gcgctaatga ggattacttc tttcgaaatg gattaaagcg gccaagatca
     1741 tgaggggcgt ctttcggcgg catcatcccg gtgcgtttaa atttataaac cccgtttagc
     1801 ttcatcgtct gcacgcccag atttaacagt gccaccagct tcagggtata cagaaacgtg
     1861 aatgccggga ccatctgaat aaaaataaat agcaaacccg cacacatcac acgcagccag
     1921 gctgcatact ccatgctcag cggccgctta aagtaatgga tttccagcaa tccgaccgtc
     1981 ttgttactga tccacaccgc tgccagtgag agtaacaaca cgatgcacac cataatgccc
     2041 agttccatga tcctcactcc ccgtcgattt gatattttga gtttctgatt tacccgcttt
     2101 ggttttgttt ttcgccttct ttttgggttt gatatggtgg aacaggaaac aatccaccag
     2161 cacccaacca ttagcatatc gggccagcag taatttctgg cccggataat gtgtgtggaa
     2221 aagcattttc acatcaacgg tattcatgat cttctgcttg cgcttcagct cctccccagg
     2281 catctcaaat tcctgaataa acggtgatga caaccgctgg cgaagcattc gaattgcact
     2341 agcgttttca tcgtgttttg ccagcctccc gaatcgcaca agcgccttat gagttacctg
     2401 aacaggcccc agatgggaat taaccagttc atactgccag tagtatcgca gatccacttt
     2461 tacccagctg atgctgtctt ccagtgcggc tttcatgatg gttcccgaca tcgggaccat
     2521 ggtcagcttg gccagacgca tctgaatgta atcagccagg ggcctaaggg actcaggtac
     2581 attgctgttc aggagaaggt gctgaagccc cggtgtcgac gtctgccagc tcaggttgcg
     2641 gccgtcaggc aaaaattcaa agttctgatc cgctggcgcc gattttgcgt tgttctcttt
     2701 ccgcatcaga aaataatgcc cacacagtaa ctcggcccgt acctggtcct ctatcgggtc
     2761 atggcgggcc ttactgagat caaccgtcaa cacaacacgg ttgatggcat cctcatggta
     2821 ttttttcacc cagacacaga cacgataaag tctctcatca agtttctggc tggtcgtgga
     2881 aatccattgc atgcttttct cctcagacct ttctcataat gaaaatgcca agatctgcac
     2941 cgggtacaag ttccgtgtag ctatggttgc tccttcgacc attggacagt gcttcatcct
     3001 gcgctggcac ccagtcctga agtcttttaa ttgttggctt ttgtccgacg ccaaaaatgg
     3061 gtaatgaggc tttcaccacc ttgtaatcag tctcggcata tttgctactt ccacgctcct
     3121 tgccttcagg ccggtatgtg aacaaatgcg ctatcggaac cgggaaggta ttggttcgac
     3181 aaaggcgata cccttcgccg tctctcagta cttttctttc cagtaatgcc ttcgctctat
     3241 cgatgttcgc caccactgtc aggtcagttg tcacgggcga tttgaccgct actaaacgac
     3301 gcttatccag catcgcgatg agatcacttg cgtttgtttt tacctggttt cggtttagcc
     3361 gccaggcaaa agtaactttt ttgagctgtt cttccacaaa cagaattttg cgagtggaat
     3421 gcgcagtcac cagaccggga agcttacggt gaacttcctt aaaccggatg tgtttctccg
     3481 aattcaccct tttcattgca gaggcaaagt cacttttggc atcatttata tcgcaaataa
     3541 ttccgctcag ctgttcggca ccctcaggaa caataatcaa acccgggtat ttggttgtca
     3601 ctttcgtcga attcctgctg agtacatcaa tgtcatcgat acagaaatcc tgaataacac
     3661 ggataatttc atccacggag gcatctgaaa tagtactcac ctcgatgggg ttcacctctt
     3721 tcaaccgctc ttcgtgtcgt gtgacggtcc ccacagtcat cagctcggcc atcaccggct
     3781 gatggtcatt cagaaaggat ctgagctttt caattcgctc cagaatccgg tcacggtaaa
     3841 agagtaaatc ttcactggcc attcatcact ccggtttcaa taaagcagcg caacaccgtt
     3901 acgtcttcaa cgacttctga agagtcgata tgcagccggt agttgtactt ttacagcacc
     3961 ttccgcaatt ccccgggatt gccggttgta taaagtggca gaggttcaac ctgctgaccg
     4021 tcatgccgaa aacaacgatt aaggtcattc aaaaggccaa aactgaccgg ctggcgttgc
     4081 agattgatgc gctgccagtc ctgctttaca ccaaacttca cccaccaacc cagaccaatc
     4141 agttcattac gtatgttctg cacattgccc aaatccgata ctgaaacatc cgggattaca
     4201 cccttccctt ccaccagcaa atgcatcacg ctctaaaaat caggctgacg gctcttcctt
     4261 gcgtgcttca gaattgtacg aatcgaacgc aagctgtggc gggaatgcaa attacgacgt
     4321 tcagccgaac taagccatct ctggataagc tcatttgaat atgacaccgg catttcaccg
     4381 gggcctgacc tttcgatttc acgcacatac agcaggcagg aagtagcggc actcactact
     4441 gcgtcagaat tttcgttcat gttcacgtcc gttaatatca gaaatctgct tatccggtgt
     4501 caggaacaaa ggctgatagt acaggcgcca cggatagtgt accgtcaaac catccgttcc
     4561 aatccggttc aaccggtagc gtccattaat tctgtcaaca gaccaggtgc caggaactac
     4621 agaacgactc cagcggaaaa ccggcagatc tccgagctga agtgtattca cgagcccttt
     4681 atccatttcg gtcacgcgta ccttcaacaa gaagcccagc cagggtaaag accagataca
     4741 cagccaaacg ggctggagga caactttgtt taacaaatga cctgtcatga acatcgctga
     4801 taaaaacata aaactgatga taatccagaa caccatccat cctaaccagg ctcttgtctc
     4861 acgaagtttc cattcaagaa ggtaaagtcg ctttttcatc cagagccccc ggaactcagt
     4921 gaatgactga tgtctgctgc atcaaaatag tggtgcgtgg caggatctcg tttcgccaca
     4981 actcttgagc cgtcgtccat ccatgtagaa ttccgttgcc acagtgtgaa ttgaacgcca
     5041 tgacctcatc agttagctga tagaccagcc tgtctggcat ttcaatgtac tccggaagcg
     5101 aaagcaggac gccgtgtatc acgtcgccgg taagcctcga caacgtctct tcgtcgtcca
     5161 gattctttgt gggcatcgtc gaaagcggga tagagaaaca acagagatcc attcccaccg
     5221 cgtcaaaacc aacatcaaaa ccttcgtcat tgagcacaaa tcggaagata gcctcaggcg
     5281 cctgttctag acccggcagc gagccctccg gcacttctgt gccggtaaaa aacttatcag
     5341 tccagttact gaatggactg gtgaacaccc aacaaagcag aggcaacgca atatccgttt
     5401 taagtactcg tttcgtgccg gacgttcctg aagagtgaat tttgcttttc attatttacc
     5461 tgtagagtgc tttacttaca gctatataaa cacattccgg aatttcccca ataggttgga
     5521 aaacactaaa attcacagat actggttccc ttctgtttta gccggtgctc aagatagata
     5581 cttgccaggt cgagtggctg attttcttcg agacaggcct gaacaaggtc ttcagtggat
     5641 acggatacca gccctcgggt ttcaagaatt tctttcgccg tcgtcaatgc tcgtgaggcg
     5701 cttcgactta gctgaatttt cacctgagaa ctgttttcac acagggcatc agctggtacg
     5761 gtctgtgaat ctgtattgct cataggggta tcactccata tcagggatcg aatgaaaatt
     5821 aactgcaata gacttctctg gttgaccgtt ttctacagtg cttagccagg ttcgagccgg
     5881 aagttgttgc tcacaaagaa gcagatagcc ctcgccattt cggtgctttt gtcccagagc
     5941 atgcaggaga tccccagaat atggacccag agctgttcgg gtataaaaca acgtcccaga
     6001 atgtatcact ttcgacaatt tgctaagcaa aacggtcttc tcgtcttcat tttcgggtaa
     6061 gacaaggatt atcctgaatc ccttggctac atgtgattgg agatctgcca ccagcgtccg
     6121 aaacctgaac gcataaataa ccaatctgaa tcgggtcatt aacagcgttg tcgcggcaaa
     6181 aataacgaca actgagccga atttagaacg gtagaaccac cagttgaggg caccggccac
     6241 tatcaaaacg accagcagca caatgcttcc gtaaaaaaat gcagccggaa aacgggttct
     6301 ttttccttcg aggaacgcag atgccagtat tacgcctgtg atgcaataca caacccagtc
     6361 aaagaacgat tcagtcatca ttaggtgata gtgtctcgtg ttgtattaaa tcggacgcct
     6421 gccgttataa agtcctcagc aatcgtgtcc catgcagcca tcaacttcgc taaaattgtc
     6481 acaactctcg gttgtaacgt aacgttcccc actagctaaa cggcgctgta aatcgatctt
     6541 tacttcagcc gccggtttga gaaacagata ttccagttgt gagtcagttc ctgaagaaag
     6601 cagtagctca atactgaaat caatatgccg gtatgtgaaa tggctattgt cattagagaa
     6661 gaccgaattc acagggttat tgctcaggat tgtagtggca ggcatagtga ctcctttgtt
     6721 ttaaacagta tacaaatctc taacgatcca tttttcagat aagagaccta ttttcgcgaa
     6781 accgaaacaa aagaagtaaa aagtaatttg gaggctccag cattcggact gtgtgctgcc
     6841 ggaaacgtca gcaaggggca aaacattcga gtaaaagaag cagagaagga aacagctgga
     6901 aggatgtaaa ttaggctagg tttggttgaa gaaggtaatg gaaggcttac atgtaacggt
     6961 gccaagagta cagtaccgta aaacatatag ggcagtaaga acgttgtaaa attgtaaaat
     7021 caatgcaagt tttgacgaaa gcaaaaaaaa aggcacggat aaccgtgcct tcttagaagt
     7081 ataaaaacct gaaaatatat aattcaacct tcaggactca tgccgcatca aagattttat
     7141 cctctgatgc tgcgatgaat tcacgtaagt tagcgaacac ctcagaaatt aattcctgac
     7201 ggtcatggat tttaatccga ccaccagcta attctgatac attcacccct tggtaaagtt
     7261 cagtaacaac ttcaccatct gtatcaagga ctctaacatt agcaaccata ggaaagtaag
     7321 attttgaagc atatccttca taaatcccaa taattgattc attattacgg cgtaaaacca
     7381 ccttgccaga cggcagcgtt gtttcatcca tataacaatc ggttaattgc tcttcgaaat
     7441 cgtcctcttt aagtgaaata tgcaaactaa gttcgtcggt aatacgacgc gtatatgaat
     7501 actcactaaa tgtataaccg gccatgttta tatcactgaa cgtgcgataa acctcataaa
     7561 gcccggatgt tatttcatct gatacatagt tgataagccc aataaaatgc gtatcccaga
     7621 atttttccag tgcaaatcgg gcgattaaaa cctgaccatc aacttccatc agtcttttgt
     7681 actcttcacg atcatagcca cctggctggg cgctgtccgc agagttactc aggatctcac
     7741 tacagacatc gtcgtattca aagcggctgg tcgcgaaatg tttaccggaa atatccagat
     7801 attgaccgga aaccaggtcg aaaacctcaa ggaagtaaac cagtcgcttc ttgagtgcat
     7861 ccgcatccag gccagccagt tgggtatcat taagagtatc cacataatgc cccgcagatt
     7921 ctcttaactc actggctgat atactgcatg taaacgaaac ttcgtcgctc tggcaatgag
     7981 agacactgat aaacatctct gtttcatcaa aatcaatacc aatgcctgcc agtactgcac
     8041 gaacgtagtt ctggttatca gtagaattag tcatcttcac ttcctcagga ttattaaagg
     8101 aagtgcccca caggggagcg acttccccag tgggtggatt aaaaatatgt actactgcgc
     8161 cattctttcc tgcaagttga tcagaatagg tagcgcggtc atttggtaga acaaagttac
     8221 tttttggaat gaagaggtaa aaaacaaggg ttaaacaaca tcccgtaact cttcgaccgc
     8281 ttcttggacc aacgcaactc tgtcacaaac ctgcactcgg ccggtaggtc ccacggaaca
     8341 gaaagtaccg tgcgatgatt cagacaaaac actcccgtca cagccagtga atcgaacggt
     8401 catcattaca ggaacatcct ttccggaact aacgccagta taaagacgaa tgattttttc
     8461 atcatcgcga tacactcccg tcgtcccttc agactccttg aaaccgatgt aaaggtctgg
     8521 catacaggcc gggtattcat ctgtattcaa atcaagatgc aaagtcaggt catcttttac
     8581 acgacgctga tattgatatt gacctaaacg gttaaatgag ttctttacag atttaattat
     8641 ctggagagaa gtattcaaac acttggagat acttttctcg aactctaaga tgaaatcacc
     8701 aaaatcagaa tggaaaaatt gcttaagtgc aaaacgggca atgagaactt taccctcaac
     8761 actcatgcga ttttgataga tttcctttct taaaacagga tctattgaat catgttcaga
     8821 ctccggtaca gaccccgcca atattaatga tgcgatatcg acaatatcta tagtactgtg
     8881 cgcgttatga taaccgcgaa ttcttatgtt ctgaaaacca gacttttcat atactttaag
     8941 aaagtatctg attttagtcc gaaggacttc agtttcagaa tacggcgcat tatgcttaat
     9001 caattctgat atatataaat ccagataaaa taacaaatca tcaagcgcca catttgcttt
     9061 aaaagaaagt tgttcaaaat gattttcttt gtcatatatt gtaatatcct cttggcttat
     9121 attcaggcca agttgacatt taagcattgc actaacaaag tttttatcat cactttcatt
     9181 aaacatattc acttcctctt taatgaagaa aggaagcaac ccccacgggg aatacgcttc
     9241 cccaatgggt aaaattaatt acatagaaag caggtcatct gaatgctctg gagcatacga
     9301 attaacttgc ttaagcgagt tatttatagc ctttcgataa agcgatgaag agtgtgactg
     9361 aaccatttta tactcagtta cgagaagagg tgatgtaaag caacctaagt tcgcattgag
     9421 ggataaagat ccaccgtcgc aagcttctat aacagcgtaa atacgccgct tcatcaccct
     9481 acccgacaga taacgattaa catggttcag gaacttatcc gctcctgaaa cactacacac
     9541 attccagtca gcagaaatta tctggtctgc gactgaaacg ggtacaggtt cgactactat
     9601 ctgggcattg atttgatggt caacactgaa aaagctggtg actacttgtg gaactgaact
     9661 ctccgcaatc ttttctgtaa ctgcgaaaat cagcggcaaa gccgttgata agcgcgagcg
     9721 taaaagttca tgcagatggt tgataaaatt atgtcctttt tcaaccatac caagtattag
     9781 atccttgtct gcattgctgg agcggtaata tacatactcc agtttttcac ggttcacacc
     9841 ctcatttaga cagtccggct caacatccac aagccgcagg tgagcagttg acactgaaac
     9901 ctcaaaccga aaggtttcaa gagtgaccgg cccagccttc aacaacgcag ctcctgagca
     9961 atattgtgct atagggtggt aatccacgtc atgtccccat aaaggctcac aatcgcgcag
    10021 atatcgcctt aaatgcggca ttaaagtccg gacaggaata tcagccttaa caactagact
    10081 ctgaatcaca cccgtttcca gattgacact ttggatcacg gcaccttcaa agcccatttt
    10141 caccagcatc gcttcaatta ttttataaat cgctttcatt ttgtagccct cggttaagag
    10201 ggaatcctcc ccaagggaag aattccctca gggattagat aagttgataa taagtattaa
    10261 tacccgttac gaatcgcgtg ttgtctttca ctcaggtatg cttcgtattc atattttccg
    10321 gatgcgaaac actctttaaa aaagacactg caaaacgcct taagatcatc tctggcatgc
    10381 atttctctat ctgacataac tttcgcatag tcagatgtgt caacatctgc taaacgccat
    10441 ccgtatttca tgtaaacacg attactgagg gcaaagcctt taagatagcg acttgcattt
    10501 ttcataatct gacgactacc tgacaagtta gacaacaacc tatcaggtgc aatcatcgtt
    10561 tcaaggatat caactggcag acccttagaa aatatttgta cattataact gccgaagcaa
    10621 tcatgatact taaagtcttt tataagacgg tctggttgat cagatgtaat cttgtttaaa
    10681 acatcaagtg ccaaacgtat accttccttc actatttcat gtaattcgaa attcacaaat
    10741 gaaaagaagg aactcaggcc atccttgaac gtgagatagt cttcacaaaa acgtccttgt
    10801 cgggtcagat gtagcaatcg ttccttatta tattcacatt cagtcacgtc gtaactatca
    10861 ccgcgtgtca gagtgaatcc ttcccttgag gaaactggaa atctaatatg gtcaattaaa
    10921 aaagaccgat tccgtttatt tttaactaca tctaactgac atttaacaca atccgcgatg
    10981 tcctggtctt caaacccagg aaacgggata tctaaaaatg ttgaactgta tgcgggggca
    11041 tacgaataaa gaaaatcaaa tgaaacatcg gcgtcacagc atattccagt aaatttaccg
    11101 tcggtgccac tcaagcagat aacattactg accttcactg aatgtttacg cgatatcaga
    11161 aatgcattaa tgattttctc aataattttc atgtaatcct cacttaaata aaaaatggga
    11221 ttacacccat cagggatgta atccccggat gggtaatgtt aatcagaaat cgtaaagacc
    11281 taaatcttta tttcgccccg aaatcgtgga agatataagg cgattgacac gtttctgtat
    11341 attatgctca gatgtctctt ttataaaaag tggtaatgac ttaccaagag accgtccatt
    11401 ttttgaatat tgacagacta tatagccatg tctccaacca gtactgatat aagactgaat
    11461 gcatttgata aacccagaag ccgccgcttc tgttcgtaaa gaggcatatg atttcagata
    11521 cctttctatt gaacatagtg caacagcttt aagagtcacc tccagcattt cattcccgtg
    11581 cttaactctc ttaatcaact gatcttcatt ccgttgcagg tatacatttc gcagtcctgc
    11641 aatcaaaatt aaaacctgtc tgagtaatgc acagagccta ttacgaacat atttcatgaa
    11701 atcttcaaaa tacaaaagcc agaaacaagc cgcctgactt attaagattt catgcgagca
    11761 agacagcatc ttagccagct cgatatcttc agttcgaaaa gaaaagttac ttttctcaaa
    11821 ccgctgttgc tttgaattac acgtatagag ctctatccat acagagataa actcaagtga
    11881 ctccatgtct tcacagagca attcagcact gttgatacac tgctgaagtt caaggccgat
    11941 atcatacgaa accggacaat ctttcagaga ctcaacgtag tcagggacga actgagataa
    12001 atgcgttgtt ttcagataca ggctgagatg cacttgtgtc aatggcttta ccagcgacgt
    12061 tacaggcaac tcattaatgg aagttggtac aatgccattc tttatcagaa ccgcattgac
    12121 cagatctgta gctaatttca taagacctcc agattcaact ctgagggcca ccccgatggg
    12181 aggtgccatc agggatgtag taaaaatatg tgttatcttt atgcgcaaag tgccatgaca
    12241 ttgtcgatat aactgacaag tgatggtcgc tcatccttta ctgcaaagtg acgaaggcca
    12301 tcaaggttgt atgctggaag gaaatcggca taccctcgcg cacgaagatc atcaataaca
    12361 agttttaaat aatcaaattt accaagtgaa cctcttaatg gaggtcttcc cggcttggac
    12421 aaaatttcaa aagcttgctg ccaggtgatt tcttccgctt ctcgtgttgc aagattaaaa
    12481 tgcacatact taaatgcttt ataatctcca cgacttgtaa gatagtaatt cggaaagaag
    12541 tatttatcac aacgcaacaa acaggttcct gaaggacgaa taacgtacaa caggtttttg
    12601 tctttatcta ttatgctgtg ttttaaaatc gtgaagtcaa tttcattatc atgaaggtaa
    12661 taacacgaag atggttgctc aacgatatga gatgaaatca cagcagcgaa cgtattaaat
    12721 aaatcttgat tttcaatatt cataacaatg ctctccgata tttaacgatg agcatcaccc
    12781 taacggtaat actcccgtca ggggttttaa gttttatgga tataccatgt catacaaccg
    12841 accgtagcca gatgtatcgc acatttatta aactgagcaa gcatctcagc cgtaaaaaaa
    12901 acctgaataa caggtgattt tcacgaagga aatcggatta tctgtttagg atttaacgta
    12961 aataaacact aaagagataa ccccccccgg cctgaaaagg cgggggagtt atcggattta
    13021 aagctgagca agacattcag gagcccattt tacggggaat ccatccggtc ctgttaattt
    13081 tagtacatcg ttccagtcga ctcctttctt ccctacagga atatcgaaat caggttcaaa
    13141 gactttaatt gtcagctctg aagtgggata tcgctttgca aaaaactctt tcatacgttc
    13201 ctgaagtcgt ttagctgact ccattcccgg ggaagtgcct tttgtattca caagatcctt
    13261 atccgcccag atgtaaaact gtattacttt cacaccaggg ggaggcagga gactatccgg
    13321 cacttcaacg ttttcaaggc accaggcaga gctcgcggcc cagcacggtg ttgaagtcgc
    13381 ttcagtaact gacagcgcgt tttctatgcc ttcagccaca cctaacgtcc aggtacgggt
    13441 acctggattg aagtgtggat cgtacagctg aattgagccg ccagtcatat cggcaggcgg
    13501 tttcatcatc agtttcggat tctcaacatg tgccttatca ccatttaact cgacgaacgt
    13561 tctgtggatt gtcagaggac gcccacgggt atcgcgataa attgcgatca ttcccgggta
    13621 aatcgtgggt ttttccagtg acttatccat gtagtaaagt cgtttgttca gacctaaatc
    13681 cttgggaagg ttgctcagaa ctcgattaag acctcgattt cggagataca cccacacagg
    13741 gctctcaggt ttgatttcac cggtatagtg atatatcact tcaagtttac gccctcgttt
    13801 ttcaacttca tcgctgtcaa gcgtttcggt tgcacgaact gttgatttat aacgacgttc
    13861 actttcgaga tccgcatcag ttaatggagc gtacaaatcc atcccaaaga aatcagtcag
    13921 gatcttctta caagtctggg tcttgctcag cttgtagtac tctgccaata catcaatacc
    13981 gtcacaaaat gcattcactg gaaaatcatt gtgaatggca caaccggtaa agaggtcctt
    14041 tttacggaac cggaactttg atttcccgac cccactaaat gggcaaggaa cctggccagg
    14101 tgctttatca caagcgtctt taaatgccgg tattctatcg aatacatagc gccagcctga
    14161 tacttccgct atacgagcgt gaaccatttt tataaagtgg tcagtgactt catgtggtgt
    14221 acgcttaaca gcctgggatg cataagtcat ttgttctctc ttatctggca ttgtgggttt
    14281 ctcaatgccc tctgcgatgc agaaaacagg gagaaaccca caacgcagaa tctaagaatg
    14341 agggcccaca agagccctca acaattacgc ttcagtcttg aagccgatat tttcaagacg
    14401 ctggataaaa ccatcaacca ttgacgttgc catttctcca ttaccctctg gtatagatac
    14461 atcccggaga attctttctt ctggcaattc acttttgaca atttcaccca tgcgaccaaa
    14521 aatacggaca cgatgcatcg tcccgacttt ttcatactcg acacggtaag tctttatctg
    14581 agtcatatat tgctccttaa tccttcaggg agcaaagcga cccaaccggg cgtcgttttg
    14641 ccccgattgg gtagagttta attagataga atttgatgta ctaatttact gacctcattc
    14701 ggcaataaag aaatatcatc aacatttacg aaggtgccct cattaaaacc catcaacaca
    14761 tcagttttaa taccaacacc agcgatttca atgccgttac tttcagcaac tgaaaaaaca
    14821 tcattaatgg tgtaagccga ctcagtcgga taaccatcag taataaggaa aacaattttc
    14881 ctgtcaaact ggctttcaag taacaactct aaagcagcat ttaatgcaga acccgttggg
    14941 gtattattgc ccttgcagcc aagtgagaat tttgaaagtt tctcttccgc gttttcatca
    15001 aacgttttga taacttcaaa atctttgtct acaaacggat aaatcacatt agcaacatgc
    15061 atttttgaaa tagcttcaat tgccagagtt aatgcaaggt cagtcttaat tgcatggata
    15121 taacggtgtt ccagactcat tgaacctgaa atatcacgaa caatgaccaa gccgacgtgg
    15181 ccccggtttt tagattcgct ttgtgaccga aatacatccc taacccccat cggagactgg
    15241 atcagtcgat taatatcgaa tgacaagcct ctatcacggg ttttaaattg accacgcatt
    15301 tttacctgct gtaaaacaga taaatctgcc ccaagggtct ggctgatatt taatgcttca
    15361 ttgtagacat tcagatcaat gttcaaatct ggaacatccc actcacttgc accaaattca
    15421 gctttaacct catccgacac actggcagca attacggaga tctcttttgc aagaacctca
    15481 tgaaaatcct cgctttcctc atcactgttt aaaaaatcat ccagcaattt ggcaagcata
    15541 tcccactgtt caggagaaaa gttactttcc ccatcagaag aatttccatt tgaactgtcg
    15601 tttagggaag gtgtcgagtc agcatcatcc gagccggggg tcgccgattc atcaccttca
    15661 gactcaccat caggttcgcc gttagagttc tcagcatttt gttcatcccc tccggattgg
    15721 tccggtaacc cattaccttc cgcaggcgcc ttaccatcac cagaatcatc gccttgacct
    15781 tctttatcac catccccttc accctggcta tcaccattgg gttcggtatc aggttcattc
    15841 tcagagccag atgattcatc tgtatcgtca tgcgggtctt tcgactgctc ctcttcttgc
    15901 tgtttctctt ttgcttcatc ccttaaacgc tctaaaagag ccagagtttt acgcgccaga
    15961 gaatcgcaat tttgcgtact tttgcaactt agagcttgat gaatgatctc atcgacttct
    16021 gaagttacag gtgcaaggat tttctttgca taatcgaaaa atgggtcaag cgtttcttta
    16081 tgcccttctt gttggagaac gttaacccgc aatgtattaa gcaggtacat ttcaataaac
    16141 tgaaccgggt tttgaggggt tgaagaaata tcaaccgtca tacgcccagc ttcaaccatc
    16201 aaggcataag tcaccgcgag gcgttttttt gcctcctgat aatcaatacc cgtcttctct
    16261 tccatctgga catcatcaaa aagattaata agtcctttta gtcgacaagc acgagcatat
    16321 gctttttgct tttgttttag gttttgaaaa tcagccttca agtcatcatc aatactgatg
    16381 aagccatctg cattgatcaa ttctccaaca aaagcttcac ggtatacttc atgctctgta
    16441 taacgaccgt gtcctgcttc atgacatatg aatccttcaa ttagcttgac aaatctctca
    16501 tcgctataat caccgttagg taaaacacag acattgtttt gggtgttgaa ataaggtgta
    16561 ctcaccttat tgtcaataat tacataaatc ccatttatac cgcttatcac gctggctata
    16621 ttcgtcaggc gttgaatatg gcgtggtgaa gattttaaat caaatttcat ttgagtacct
    16681 ttatttgata aagggtactc acccagatgg tgaagtaccc atctggggat atttttacaa
    16741 gactaaatta ccaaccggtc aattcgagtt cgcagtcaaa tgctctatta ctaccactgg
    16801 cctgaataga aacatcttca tcagggccaa cagcaggctc aggtgcattc actgccttgt
    16861 tctcaagatc ttcaaccggg tcttctctga aaacatttgc tgaagaaaaa acatcatcct
    16921 cagcagtatt cagagagtta gccgctgtaa cctgtgaatc gatattgaat gaccgggtta
    16981 tggaatccag ccagtcggtt accttttgtt caccggagat aatacgtttc agcattgacg
    17041 catcgctcat gaaatagaac cactgcttta ataccgcaac atcacgggga gaagaaatgg
    17101 caccttttgg taaattattc atgatatgct taatagcttc tgcaagaaca acagcctctt
    17161 tatgaatgaa catgaagcta attattttat cctgcatctg ctttaatcgg tcaatcgtgc
    17221 tggaacgcaa attatcattt ttgctgattt ttgtataaac cttcatagct tcatcggcca
    17281 agtcagttat cagttgttct tgataaaagt cctgcgtttc atccccctcc acactaatcg
    17341 gtgaaaacgg aatgaaatgt gccagattaa accggaatga tttaacaaaa tcatctttag
    17401 tcaatttcaa ctggtcaatt aaatctgaca tcaacggact gcgatctttc tcagcctgaa
    17461 tatgagtgtc atagttatca accacgttct ggataccggc ttgaaattca cgttcgattt
    17521 cttgtgcctc ttcgagaaac tcctccatcc tttcaactgg cagtatccac atattgctat
    17581 agcggatgca caaccgtgaa caaagggttg aaattcgact agtgatagtg cttttgaaac
    17641 tcagtgcctt tatgtctatc cagcgaactt cgcttttaga cacgctcttc tggtctgtta
    17701 tatcatcacc gccataggac agttcgattt tatcagttcg ccgagagccc tgacacccac
    17761 taatttcaat ttttgttata acagcacctt cctgcaagtc aggatatgct tttataaagt
    17821 tttcactgat ttcctttgca gaaactttat taagtttaga agtattgaaa gtcataggtt
    17881 ttctccagat ttagaaatgg gaaaacctcc ccgtcgggaa agatttcccg atagggttaa
    17941 taatattact tttcaagtgt tgttaaaaga attccaggaa attcattttc gacatccgcc
    18001 gattgaatgc tcccttgata catgaatcct tttgattcaa ggcccttaat catggcttcc
    18061 atatctttgt tacagacttc ataacaaata aatgaatagt cattattatg ctgcttcaga
    18121 cctatatatt gatttccatc ttgtgcaatc acgaaagttt cagttccgtt tgcactcaga
    18181 aaaccgtaaa acgtaagatg agcaaagtct gtcacacggc attcagcctt ctgataactc
    18241 gtcccatcta taactacttt tttgcagtgt aatactccat tgtacggttc ttgtcgtcac
    18301 cgagaattaa gtcgatggct tccaacacca taccagtggc atgtgcaggc agagccgacg
    18361 caaaagcaat tttaaatgta tccagtaagg acatggtctt taacgtcatt gacgttccaa
    18421 cccatgccac aagctggcgt atatccatta cactgtcgtc agttcctgtg ttgtttactg
    18481 caacggctac cgaaacgaat ttttcaatta ccagcatagg caggttattg tagcgtttaa
    18541 gcaaaacgtt ggttaatgca acctctgaag gcctttccat ctccacaatc aggaagcgct
    18601 tgataaacgc tgcatcctgc cgctgcgatg aaacgaactt acgagatccc ccactcatgt
    18661 ttgtgtttga agtacccata aaccgcgtaa acttatttgc cttaaccact tcgccaccgt
    18721 tggcagctag tgaccagggc tttcgctcta taggcatatg aagctttgcg gcagtatcag
    18781 ggtcaatctt gtccacttca tccagaagaa tgaggtggcc ttcctgaaat gccgttacaa
    18841 gctcgctagg cacaaaactg gtgttaccgt taataagact acgctcacct tccagctcat
    18901 cagcgcgaat attgctgttg atttgcttta tggttagcgg ccagttcagc atatggctaa
    18961 tatacattgc catttcagtc ttacctgatc cagattctcc tttcaggcat acactcaaat
    19021 ctatatcgtt acttacaagc aattttacta cttgctcaag tattcgagaa tttggtatat
    19081 agtccttatt cagattcgca cgatatggag aaaacttttt aaacactgga acaatcatat
    19141 cctcatccat ctcaggaatg ccaaataaat cttttgcttt gacaagataa tactgttctt
    19201 tattcgcaaa catcttactt gatataacat ttgcgggtaa cagattacca atgatttctt
    19261 cctgtacgga attgtcggat tttaatacag ttgcggcgtt agtcattttt aaaactccgg
    19321 taaaatgcaa acaccgcccg ctggcagatg tttgccagtg gcatgttagt ggaaagtcat
    19381 cctacacgcg gtcgccatct gtgttctgat agcggcaacg ttgtaaccaa gatgagttcc
    19441 tacgatttcg aatacttctg actcaatcca ttcaaactgg cgttcaaatg tagggtttac
    19501 gattacccaa ccaa
//
LOCUS       DAMZII010000091        19360 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZII010000091 DAMZII010000000
VERSION     DAMZII010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.091"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(152..1372)
                     /gene="fsr"
                     /locus_tag="POA77_003159"
     CDS             complement(152..1372)
                     /gene="fsr"
                     /locus_tag="POA77_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDI6366546.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARVASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1590..3242
                     /gene="ushA"
                     /locus_tag="POA77_003160"
     CDS             1590..3242
                     /gene="ushA"
                     /locus_tag="POA77_003160"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDI6366547.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3279..3758)
                     /gene="ybaK"
                     /locus_tag="POA77_003161"
     CDS             complement(3279..3758)
                     /gene="ybaK"
                     /locus_tag="POA77_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDI6366548.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3962..4756)
                     /locus_tag="POA77_003162"
     CDS             complement(3962..4756)
                     /locus_tag="POA77_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDI6366549.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4894..5235
                     /locus_tag="POA77_003163"
     CDS             4894..5235
                     /locus_tag="POA77_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDI6366550.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5349..7853)
                     /gene="copA"
                     /locus_tag="POA77_003164"
     CDS             complement(5349..7853)
                     /gene="copA"
                     /locus_tag="POA77_003164"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDI6366551.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8115..9047
                     /gene="glsA"
                     /locus_tag="POA77_003165"
     CDS             8115..9047
                     /gene="glsA"
                     /locus_tag="POA77_003165"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDI6366552.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            9050..10342
                     /locus_tag="POA77_003166"
     CDS             9050..10342
                     /locus_tag="POA77_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6366553.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     LGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10467..10874
                     /gene="cueR"
                     /locus_tag="POA77_003167"
     CDS             10467..10874
                     /gene="cueR"
                     /locus_tag="POA77_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDI6366554.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10875..11333)
                     /locus_tag="POA77_003168"
     CDS             complement(10875..11333)
                     /locus_tag="POA77_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDI6366555.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11330..12247)
                     /locus_tag="POA77_003169"
     CDS             complement(11330..12247)
                     /locus_tag="POA77_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDI6366556.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12393..13070
                     /gene="fetA"
                     /locus_tag="POA77_003170"
     CDS             12393..13070
                     /gene="fetA"
                     /locus_tag="POA77_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDI6366557.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13057..13836
                     /gene="fetB"
                     /locus_tag="POA77_003171"
     CDS             13057..13836
                     /gene="fetB"
                     /locus_tag="POA77_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI6366558.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13899..14753)
                     /gene="cnoX"
                     /locus_tag="POA77_003172"
     CDS             complement(13899..14753)
                     /gene="cnoX"
                     /locus_tag="POA77_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDI6366559.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14814..15623)
                     /gene="ybbO"
                     /locus_tag="POA77_003173"
     CDS             complement(14814..15623)
                     /gene="ybbO"
                     /locus_tag="POA77_003173"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI6366560.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15613..16236)
                     /gene="tesA"
                     /locus_tag="POA77_003174"
     CDS             complement(15613..16236)
                     /gene="tesA"
                     /locus_tag="POA77_003174"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDI6366561.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16207..16893
                     /locus_tag="POA77_003175"
     CDS             16207..16893
                     /locus_tag="POA77_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6366562.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16890..19304
                     /locus_tag="POA77_003176"
     CDS             16890..19304
                     /locus_tag="POA77_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366563.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGTIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         4511 a         5038 c         5274 g         4537 t
ORIGIN      
        1 gatgttcatg aataattatt gaattttgca gaaaaatgga attagctgca aaaaaagcac
       61 ggatcggaaa acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg
      121 cagccaaagt ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga
      181 atatggtcaa catccccaat agtggcagga aagcacagat tttatagact aactcgatac
      241 tggtgtgatc ggcgataagc cccagaactg ccgctcccag acctcccata ccaaaagcaa
      301 aaccgaaaaa gagtccagaa accataccga tacgtcccgg aagcagctcc tgagcgtaaa
      361 ccagaatggc agagaatgcc gaagcgagga taaatccaat aatcaccgtt aaaacccccg
      421 tccagtgcag gctggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc
      481 cccaaatcac atatttccgc ccaattttat cccctacagg cccgccgatc accgtacctg
      541 ccgcaacggc aaacaggaag gcaaacagat gaagctgagc attctggata gataatccga
      601 atttttgcat cagataaaag gtgtaatagc tgctgatgct cgccatatag aaatatttcg
      661 agaaaatgag gattaacaga atgctgaccg ccagtaccac tttgttgcgc ggcagtggat
      721 tgataatcgt cgctttgggt tttcctttat tcattcggtg ctgtgccgag taccaacggc
      781 tgatttgcgc caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc
      841 ctttgccata aggcgcgata atcaccgccg ccagcaaggg tcccagggaa ctgccaaagt
      901 tgccgccgac ctgaaagata gattgcgcca ggccatgccg cccgccggaa gccacacggg
      961 ctacgcgaga agattccgga tgaaagaccg atgaaccggt accgaccagc gccgccgcca
     1021 gcagaactgc gccaaaactg cccgccagcg caagcagcac cagaccactt aaggtaaagc
     1081 acatgccaat tggcaacgac cacggcatcg gatatttatc ggtccagtag ccgaccactg
     1141 gttgcagtag cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg
     1201 tcagagaaaa ttctgactga agcagcggat aaatcgccag aatcagcgat tggatcatgt
     1261 cgttcagcag atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg
     1321 tcgttgacgc agccgcgccc gccacaggct ggggttgttc actcattgcc ataggaaagt
     1381 cactttttca gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca
     1441 tacctgtatg cgtcgcctga aggaagtctc aacgccgaat acagaatttc taatctggat
     1501 gcagatttat cttcaccgga cgcagacttg tctatgatgt cgcgtcatac tatttttcaa
     1561 cacgttgaaa tcaggtcagg gagagaagta tgaaattatt gcagcggggc gtggcgttag
     1621 cgctgttaac cacatttaca ctggcgagtg aaactgctct ggcgtatgag caggataaaa
     1681 cctacaaaat tacagttctg cataccaatg atcatcatgg gcatttttgg cgcaatgaat
     1741 atggcgaata tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg
     1801 cggctgaagg cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg
     1861 agtctgactt acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg
     1921 cgatggcgat cggtaatcat gaatttgata atccgctcac cgtattacgc cagcaggaaa
     1981 agtgggccaa gttcccgttg ctttccgcga atatctacca gaaaagtact ggcgagcgcc
     2041 tgtttaaacc gtgggcgctg tttaagcgtc aggatctgaa aattgccgtt attgggctga
     2101 caaccgatga cacagcaaaa attggtaacc cggaatactt cactgatatc gaatttcgta
     2161 agcccgccga tgaagcgaag ctggtgattc aggagctgca acagacagaa aagccagaca
     2221 ttattatcgc ggcgacccat atggggcatt acgataatgg tgagcacggc tctaacgcac
     2281 cgggcgatgt ggagatggca cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg
     2341 gtcactcgca agatccggtc tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc
     2401 cgggtacgcc atgcaaacca gatcaacaaa acggcatctg gattgtgcag gcgcatgagt
     2461 ggggcaaata cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg aaaatggtta
     2521 actaccagct gattccggtg aacctgaaga agaaagtgac ctgggaagac gggaaaagcg
     2581 agcgcgtgct ttacactcct gaaatcgctg aaaaccagca aatgatctcg ctgttatcac
     2641 cgttccagaa caaaggcaaa gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc
     2701 tggaaggcga tcgtgacaaa gtgcgttttg tacagaccaa tatggggcgg ttgattctgg
     2761 cagcccaaat ggatcgcact ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg
     2821 attctatcga agcaggcgat atcagctata aaaacgtgct gaaagtgcag ccattcggca
     2881 atgtggtggt gtatgccgac atgaccggta aagaggtgat tgattacctg accgccgtcg
     2941 cgcagatgaa gccagattca ggtgcctacc cgcaatttgc caacgttagc tttgtggcga
     3001 aagacggcaa actgaacgac cttaaaatca aaggcgaacc ggtcgatccg gcgaaaactt
     3061 accgtatggc gacattaaac ttcaatgcca ccggcggtga tggatatccg cgccttgata
     3121 acaaaccggg ctatgtgaat accggcttta ttgatgccga agtgctgaaa gcgtatatcc
     3181 agaaaagctc gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt
     3241 aatccgaaag tgccggatgt ttgcatccgg cacaatgctt aatcgcggcg ggcgatatca
     3301 gcaaatttgg catcgaggat ctttgccaga tcgcctgccg ccagttcgat atccagtccg
     3361 cgcttgccgc cggaaacata aatagtggca aattcttgtg cgggggcgtc gataatcgtt
     3421 ggcagacgtt ttttctgccc cagtgggcta atccccccaa ccaggtatcc cgtcgaacgc
     3481 tgcgcggcca tcggatcggc catctcaact ttcttggcac ccagcgcttt tgctactttt
     3541 ttaagatcca gttgacctgc gaccggcgta acggccacgg caaggtgttt catatcaccg
     3601 ttcactgcca ccagcagcgt tttgtagacc tgatccggat tcaaacctaa ttttttgacg
     3661 acttcatcgc caaaattggt ttcagccgga tcgtgctcgt aggtatggat ttgaaacgaa
     3721 atcttgtttt tttcgagtaa tttaactgcg ggtgtcatgg taattttcct gccttaaaca
     3781 aaaaaacgaa gcaagcatac gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag
     3841 agtattggca ggatggtgag attgagcgac aatcgagtta caccgtcgct taaagtgacg
     3901 gcataataat aaaaaaatga aattcctctt tgacgggcca atagcgatat tggccatttt
     3961 tttagcgcaa catttgcggc aaattccctt ctccatacag gtgtagtgca ccgaccgcga
     4021 ccacatatcg ccccggcggc atggcgcgta atttatcccg ccaggcgaga tttcgctgat
     4081 gcatcagcac atcgtacagc gactgactga acgtattggg cagcgttata tcattatttt
     4141 gcggaggtgc attcagccac cagctcatca tttgttgcag caaccgtgcg ttggtatgcc
     4201 agtgggtcag cgtatcgtcc agcagcgcca gtcctttgtc agggagctgg agcaacatgg
     4261 caatctggtt ttcagcccct tccagttcaa tcacgggttt atgttgttgc ttcgccgcct
     4321 gcaatagctg gtaatcgata ccgtattctg cccgcagccc cagtttttgt gcctgcgtcg
     4381 cctgaagaac catcgcgatt tgccacagcg gttgggtaga aaagagtgag ggagaaatgc
     4441 ccatctcctg gctaatgtgc tgcaggtttt ggagttgctc ctcgctaatg cgctcttcca
     4501 gcgcctcgca ggcaggcaaa ttagcaaaag gcgtatcgct ggtggaaaca tccgcctcga
     4561 cgatcagcgc atcggcgttt ttgagctttt tgagcaaacg ggtgggcagg ggagccatat
     4621 cgtggctacc catatgaata ctgccaatca gatgaaaatg gcgattgccg gggagggtga
     4681 tatcgatggc aggccaggag taatgattac cgcgcagcgc ggcccaaagc gtttttaccc
     4741 ggtacaacag atccatacga cctccctttg tgaaatatca tgctagcgcg cggggaggga
     4801 tggcgcaaat ggagttgggg acttgtttaa tgtgtttgta tgattcagta tgttcttgta
     4861 tcgctattca cagggaagca acagttaaaa actatgaaac aggcaacaag aaaaccgacg
     4921 acacccggag atattcttct ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag
     4981 ttagcagagt tgctgcatgt tcatcgtaat agcgtcagta cactgatcaa taataatcgt
     5041 aaactcacca ctgagatggc attccgtctg gcgaaagttt ttgataccac agtcgatttt
     5101 aggctaaacc tccaggcggc agttgatctt tgggaagttg aaaacaacat gcgcacccag
     5161 gaagaattgg gacggattga aacagtggct gaatatttgg cacgccgtga agagcgtgca
     5221 aaaaaggtcg cgtaaggcca aaacatcgct tcatctcgta ggccggataa ggcgtttacg
     5281 ccgcatccgg cagtcatgcg tcgatgccaa atgcgccgcc ctaaagcagc gcatccgcaa
     5341 tgacgtactt attccttcgg tttaaaccgc agcaaccggt tggcgttact cactacggta
     5401 atcgacgaga gcgccattgc cgctccggca actaccgggt taagcagtgt tccagtaaac
     5461 ggccacaaaa taccggcggc gaccggaata ccgatgctgt tgtagataaa ggcaccgagc
     5521 aggttctgct tcatgttgcg cagcgttgcg cgggaaatcg cgagcgcatc cgcaacgccc
     5581 atcaggctat ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc actgccgcca
     5641 cccatcgcaa tgccgacatc cgcctgggcc agcgctggcg cgtcgttaat gccgtcgccc
     5701 accattgcca cctgacgtcc ttcactttgc agacgtttga tcgcttcggc tttaccgtcc
     5761 ggcagcactc cggcaatcac ttcatcaatt ccagcttctt tggcgatcgc attggcggtg
     5821 gttgggttat ccccggtcaa catcaccaga cgatatcccg ctttatgcag gcgttgcagc
     5881 gccgccacgc tatcactacg caacggatcg cgtacagcca gcagggctac tgctttcccg
     5941 tcaatcgcca gcagcacagg cgttgctcct tgcgatgcct gagcagtaat ctccgcttcg
     6001 atagctttgg taccaacctg ttgctcattt aacagcgcct gattgcccag caataacgca
     6061 tgaccttcag cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc gttgacctgc
     6121 ggcagctgca tatcacctgc tttatcgagg atcgcccgtg ccagcggatg gctggaacct
     6181 tgctccagtg ccgccgccag acgcaatgcc tgcgcttcat caacatcagc aaacgttttc
     6241 actgcgacaa cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa cacgacggta
     6301 tcgagtgtac tggcgcgttg cagcgcgtcg gcgtcccgca ccagcacgcc aaactcagcc
     6361 gcccgcccga cgccggaaat aatcgacatc ggcgtcgcca gccccagcgc acacggacag
     6421 gcgataatca gtaccgtggt ggcaatcacc agggtataaa caatttgcgg tgccggacca
     6481 aagaagtacc agattgccgc actgaccagc gcaatcacca ccaccacagg tacaaatacg
     6541 gctgagattt tatccgccag ctgaccgatt tctggcttgc tgctctgggc ctggcgcacc
     6601 atgcgaatga tccgtgacag cgtggtatgg ctgccaaccg cactggcacg aaacagcaca
     6661 ctgccgtcct gtaccactgt cccggcatgg acgctttcgc cttcgccttt ttgctgtggg
     6721 attggttcgc ccgtcagcat cgcttcatcc agccatgctt cgccctgggt aatctcgcca
     6781 tctaccggca cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg cacttccgcc
     6841 agaggcacgc ttttttcacc ttcgtcagta accaggcgcg ccgtcggtgg ggttaaatcg
     6901 agtaatttct ccagcgcctt agaagaacgc tgacgtgcgc gcgcttccag catatggccg
     6961 agattaatca gaccgataat catcgcactg gcttcatagt aaagatgccg cgcttccatc
     7021 gggaaccact gcggccacag gttgacgctc atcgaataga gccacgccac gccagtaccc
     7081 agcgccacca gcgtatccat cgtcgctgca ccgttcagca ggcttttcca tgcactgcgg
     7141 taaaaatggc cgccggcgaa aaccatcacc gccagggtta tcaggccgat aaccaaccac
     7201 aggctgcggt tgtcagcggt gaccatcatg ttatcgccaa tcatccccca gaccatcacc
     7261 gggataccca ccgccagtgc gacaattgcc tgccagcgga agcgcttcat cgtagcaacg
     7321 gcggtttcct gctggcgctc acggcgtttc gcgtcatctt caatcgcttc cgcgccgtag
     7381 cccgcttttt ccaccgcctg cactaaatct tgtggggagg cactgcccat caccagcgca
     7441 gtacgctccg ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg cagcgcattt
     7501 tgtacgcggg tgacacagct ggcgcagctc atgccgctca gcagcaactg ctggctgtca
     7561 tcgtcatcgg cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc ttccgacggg
     7621 attgatgact ccgccagcgg tttagccttt gggtggctta cagatgcgtc ataacccgct
     7681 tgtttgatgg tttcgatcag ctgttctgca ctggcagtcc cggtaacgtg cgcttcagtg
     7741 atagacacat ccgcttgttc cacatccggg cgctgttcga gactttcttt cacgcgttta
     7801 acgcagtgac cgcaggacag gccgtccagg gtcaggtcga taatttgtga cataaaacac
     7861 tcctttaaga cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt
     7921 caagaaatta ataaaccagg cgagtaaaag tccgtaaaga ttaaaaaatt ggctcaattt
     7981 gcatcaggat tagacattta tctctttgtt ttcctgtagt taagttgcgg gtgctaagtt
     8041 aaatcaggat gcctgaaaat cggcaccggg gtgaggaatt acctcccgca tctataaaaa
     8101 ggagttaaca aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca
     8161 cccaatttca ctcacttaac ggcggtcaaa atgccgacta cattcccttt ctggcgaatg
     8221 tgcctggtca actggcggca gtggctatcg tgacctgcga tggcaaggtc tatagtgcgg
     8281 gtgacagtga ttatcgcttt gcgctggaat ccatctcgaa agtctgtacg ttagcccttg
     8341 cgttagagga tgtcggcccg caggcggtac aggacaaaat tggcgctgac ccgaccggat
     8401 tgccctttaa ctcagttatc gccttagagt tgcatggcgg caaaccgctt tcgccactgg
     8461 taaatgctgg cgccattgcc accaccagcc tgattaacgc tgaaaatgtt gaacaacgct
     8521 ggcagcgaat tttacatatc caacagcaac tggctggtga gcaggtagcg ctctctgacg
     8581 aagtcaacca gtcggaacaa acaaccaact tccataaccg ggccattgcc tggctgctgt
     8641 actccgccgg atatctctat tgtgatgcaa tggaagcctg tgacgtgtac acccgtcagt
     8701 gctccacgct cctcaatact gttgaactgg caacacttgg cgcgacgctg gcggcggggg
     8761 gagtgaatcc gctgacgcat aaacgcgttc ttcaggccga taacgtgccg tacattctgg
     8821 ccgaaatgat gatggaaggg ctgtatggtc gctccggtga ctgggcatat cgcgttggtt
     8881 taccgggcaa aagcggtgta ggtggcggta ttctggcggt cgtccctggt gtgatgggaa
     8941 ttgccgcgtt ctcaccaccg ctggacgaag aaggcaacag tgttcgcggt caaaaaatgg
     9001 tggcatcggt cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac
     9061 ggaaggtaat aacggtaaca aacccctcgg tctatggaac gtcgtttcca tcggcattgg
     9121 ggcaatggtg ggggcgggga tcttcgcgct gctggggcag gctgcgttgc tgatggaagc
     9181 ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg gttatgccta
     9241 tgcgcgtctg ggggcgagct atcccagcaa tggcggcatt atcgacttct ttcgtcgcgg
     9301 attaggcaac ggcgtctttt cgctggcgct ctcgttactg tacctgttga cgctggcggt
     9361 gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg
     9421 cagccaggag gagcacctta ttttgctcta cgcgttgggg atcattgcgg tgatgacgct
     9481 tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa
     9541 aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat
     9601 ttccgtctct gcgcccccca gctccggtgc gttcttctcc tgtatcggga taaccttcct
     9661 tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccgcaggt
     9721 cattatgccg cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct
     9781 ggcactggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg ccgataccgc
     9841 cgtagcgcag gctgcttctc cgctgctcgg acatgtgggt tatgtgatcg tcgtcatcgg
     9901 cgctttactg gcgacggctt cagccattaa cgcgaacctg ttcgccgtgt ttaacatcat
     9961 ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg aataaatccc tgtggcggca
    10021 gagtacctgg ggcaacatca ttgtcgtggt gttgattatg ctgatgacgg cggcgctaaa
    10081 tttaggctca ctcgccagtg ttgccagcgc cacctttttg atttgctacc tggcggtgtt
    10141 tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg
    10201 tacgttggtg atgttgttgg tgatccttgg ctttatctac agtctgtggt cccagggtag
    10261 ccgtgcgttg atatggatta ttggctcact cttactcagc cttattgtgg caatggtcat
    10321 gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acttcctgta ttattgtggt
    10381 ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa accctttaac
    10441 aaagcacagg aggcgttgcg cgaacgatga acatcagcga tgtagcaaaa attaccggcc
    10501 tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca
    10561 gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc
    10621 aggcacggca ggtgggcttt aacctggaag agagcggcga gctggtgaat ctgtttaacg
    10681 acccgcagcg gcacagcgcc gacgtcaaac ggcgcacgct ggagaaggtg gcggagatcg
    10741 aacgacacat tgaggagctg caatccatgc gcgaccagct gctggcactg gcgaatgcct
    10801 gccctggcga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc
    10861 atcgggcagg gtgattaaga cgagacggct cggatatgta gggttatccc ttctatcgca
    10921 atgacttcaa catgcgtacc tgcaccgaga tcctcgctgg cgctgacagg ccatgaactg
    10981 tcaccgacgc gcatatgacc gcgcccgttg accagcggag attccagcac aaaacgtcgg
    11041 ccaatcagct gctgcccgcg ctggtttaaa tgactatcgc tgtgcttttg ttcgcgcacc
    11101 cgccgcgaca accatttcca ccacagccag gcggcgagca gcgtcaggat ggcaaacatt
    11161 accccttgcc actcccaacc cagcggcacc agccagacca ccaggccggt aatcaccgct
    11221 gccacgccgc tccacaacaa ataaccattt ccgcccagca tctcggctgc cagcagcaaa
    11281 ccgccgagac tgagccagaa aatatgtgga tgaacgacca ttaactccat catggctgag
    11341 tccgtttgtt ggcgctgtct ttcaccagct cggcaatccc ggcaatcgac cccatcaggc
    11401 tgctggcctc taatggcatc atcactactt tgctgttact ggaggaaccg atctgctgta
    11461 acgcttcggt gtatttctgc gctacgaagt agttcaccgc ctgaatatca ccggaggcga
    11521 tagcttcaga caccattttg gtggcgcggg cttctgcttc ggcggaacgt tcacgcgctt
    11581 cagcctgtaa aaacgccgac tgacgttcgc cttccgcttt caggatttgc gactgttttt
    11641 caccttcggc tttgaggatt tccgcctgac ggatcccttc cgcttcaaga atgtaagcgc
    11701 gtttggtacg ttccgctttc atctgcgcgt tcattgaaga gataagctct gccggtgggc
    11761 gcacgtcgcg aatttcaata cgggtgactt taatccccca cgggttggtg gcttcatcga
    11821 caatacgcag caggcgtgag ttgatgctgt cgcgctgaga gagcatttcg tcaagttcca
    11881 ttgaacccaa cacggtacgg atgttagtca tggtcaggtt gatgatcgcc agctccagat
    11941 tgctgacttc ataagccgcg cgcggcgcgt caatcacctg aataaaacag actgcgtcga
    12001 tggtaacgtt ggcgttatct ttcgagataa cttcctggga agggatatcg agcacttgct
    12061 ccatcatatt gatcttgcga ccaatgcgat ccataaacgg caccaccaga ctgagccccg
    12121 gctgtaacgt tttggtatag cgaccaaaac gttctaccgt ccattgatag ccctgcggca
    12181 cgattttgac gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc gggataaaga
    12241 taagcatcgg aaaaacctcc tgttgtaccg tccataatca gcaaaattgc tgcttgatta
    12301 aacaaattat acctgattac tgaaagagag ttccccctta ttcctgcgaa ggataaactg
    12361 tttttagtaa aaatcagaaa aagggaacag tgatgcagga aaatagtcct ttgcttcagc
    12421 tacaaaacgt aggatatctg gcgggtgatg cgaagattct taataacatc aatttttcgc
    12481 tgcgtgctgg cgaatttaag ttaattaccg gtccttccgg ttgtggcaaa agtacgctgc
    12541 taaaaatagt tgcttcattg atcagcccaa ccagcggaac gttactgttt gaaggtgagg
    12601 atgtcagcac actaaagcca gaaatctacc gccagcaagt ctcttactgc gcccagacgc
    12661 cgacgctgtt tggcgatacg gtatacgata atctgatctt tccctggcag atccgtaacc
    12721 agcagcctga cccggccatt tttctcgatt ttctcgaacg cttcgctttg ccggacagca
    12781 ttttgacgaa aaatatcgcc gagctatctg gtggtgaaaa acaacgcatc tcactgattc
    12841 gtaacctgca atttatgccg aaggttttat tgctggatga aatcaccagt gcgttggatg
    12901 aaagtaataa acataacgtc aatgagatga tccatcgtta tgtgcgcgag caaaatattg
    12961 ccgtgctgtg ggtgacacac gataaagacg aaattaatca tgcggataaa gtgattacac
    13021 tgcaaccgca tgccggagaa atgcaggaag cacgctatga actcgcataa tattactaac
    13081 gaatcattgg cactggcatt aatgctggtg gtggtggcaa tcttaattag ccataaagaa
    13141 aaactggcgc tggagaaaga tattttctgg agcgtcgggc gagcgataat tcagctgatt
    13201 attgtcggct atgtgctgaa gtatattttc agcgtggatg atgccagcct gacattattg
    13261 atggtgttat ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag taaatatatt
    13321 gctaaagctt ttatctcatc gtttattgcc attacggtcg gggcgggaat taccctggcg
    13381 gtgctgattc tctctgggtc gattgaattt atcccgatgc aggtgatccc tatcgccggg
    13441 atgattgccg gtaacgccat ggtagcggtg gggttgtgtt acaacaattt agggcaacgg
    13501 gtcattagcg aacagcaaca gattcaggag aaactgagtc ttggtgcgac gccgaagcag
    13561 gcttcagcga tattgattcg cgacagtatt cgcgcggctt taattccgac ggtcgattca
    13621 gcaaaaacgg ttggcttagt gagtttacca ggaatgatgt ccgggctgat atttgccggg
    13681 attgatccgg tgaaggcgat taaatatcag attatggtga cctttatgct gctctcaacc
    13741 gccagcttgt cgaccattat tgcctgctat ttaacctatc gtaagtttta taattcgcgc
    13801 caccagttgg tggtgacgca attgaagaag aaataatgcg atgccggatg cgcagcatcg
    13861 catccggcgt tgtggttcat gtgccgatca acggaatatc aatacaacaa tgcatacagc
    13921 tggcggcgat acttcgacgc cagtgcatca cccgtaccca gcgcagcgag gatctcctgg
    13981 aacgttttac gcgtctgacc gtctgcggcg gtgagatctt tacgcagatg cccgaacagc
    14041 aactccagcg cctcttcatt gcgcccaacc tggtgcagtt gcagagccag ttgcgtcgcc
    14101 agtgcggcat cttctggatt ctccgccacc tgctgttgca actgttgaat ttccggcgta
    14161 tcagccgcct gcttcagcag ttcgatttgc gccaccagcc cctggtagcg ggtgtcctga
    14221 tcctgcaacg gaatggtttt cagcaccgct tccgcatctt cagaacggtt cagcgcaatc
    14281 agcgtttctg ccagcagcag gccgatctcc ccgttctgat tcgacaactg ccaggcgtct
    14341 ttcagcaatg gcagggcatc ggtgtaattg ccttcctgca tcagttgcat cgcctgctgc
    14401 gctttcagct cttcttcgcg cggcagcact ttatccagca gggcgcggat cgcctcttcc
    14461 ggttgcggcc cctggaagcc atctaccggt tgcccgttct ggaacagata cacggtcgga
    14521 atcgcgcgca gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc
    14581 agaataaatt gcccgttgta ctgcgccgcg aggctttcca ggatcggggt taactgcaaa
    14641 cagtgctggc tacgttcaga ccaaaaatag aacagcaccg gagtggtcat cgactgttca
    14701 agaacctgtt gcaggttaga ttcgttaatg ttgacaatat tttctacgga catggagtcg
    14761 ctctctgttg tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc aactcacccc
    14821 tgcaatattt tgtccatcac gcgccccggc agcaggcgct taagcaccat tactgcccag
    14881 gtcaccagcg ttaccggata gcgcatcttc ggcttctcgc taataaaagc atggcgtact
    14941 ttgtccacca ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg attttcgact
    15001 ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat gggaccgggt
    15061 tcgatcaggc tgactttaat tccgctgtgg cgtagctcca tgcgcagcgc gtctgaccac
    15121 gcctccagcg catatttact ggccgcgtaa gcgccacgac ccggcgtgga gattaatccc
    15181 atcaccgatg atgtcatcac aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg
    15241 cgcatggtga gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc catctgcgca
    15301 cggctgatgg tggaaagggg gccatacatg ccgaatccgg cattgttaaa gatcccatac
    15361 agacaattat cggtcagggc gatcacctcg tctgctgcgc gatcaacact ttctggtgaa
    15421 tccagatcga tcaacacgcc ggtaaatccc atgttgttca tacgctcaac atcatccggt
    15481 tttcggcaac ctgccagcac atgaaaaccc tggcgtttta attcgagcgc gctttccagg
    15541 ccaattccac tggaacatcc ggtaattaag accgattttt gcataacttt acctgtcagg
    15601 atctccgttg ctttatgagt catgatttac taaaggctgc aactgcttcg ccatccagtc
    15661 ggcaataaac ggctgggcgt cgcggttggg atgaataccg tcatcctgca tccattgtgg
    15721 cttgaggtag acctcttcca taaaaaaggg cagcagcgga acatcaaact ctttggcgag
    15781 tttggggtaa atggcgctaa aggcttcatt ataacggcga ccatagtttg caggcagacg
    15841 tatttgcatt aacaatggtt cagcgttggc ggctttgaca tcctgcaaaa tctggcgcag
    15901 cgtttgctcg gtttgctgtg gctgaaaacc acgcaaaccg tcattgccgc ccagttcaac
    15961 cagcacccaa cgcggctgat gctgtttcag cagagccgga aggcgcgcca gtccttgttg
    16021 cgaggtgtcg ccgctgatgc tggcattaac taccgacgtt ttactctgcc acttatcatt
    16081 caacaaggca ggccaggccg cgctggcaga cattcgatac ccggcgctca ggctatcacc
    16141 cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt aacaggacca ggaacaggaa
    16201 gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag
    16261 ggggagcatg aactctccat cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc
    16321 attgcactgg tgggcgagtc gggatcgggt aagtcaacct tgctggcgat cctcgccggg
    16381 ctggatgacg gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca taatatggac
    16441 gaagaagcgc gggcaaagtt gcgcgcgaag cacgtaggct ttgtttttca gtcatttatg
    16501 ttaattccta cccttaacgc cctggaaaac gtcgagcttc cggcgctgct gcgcggtgag
    16561 agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg gctgggtaaa
    16621 cgtcttgatc atcttccggc acagctttcc ggcggcgaac agcaacgagt ggcgctggcg
    16681 cgagccttta atggtcgacc tgatgtgctg tttgccgacg aacccaccgg caaccttgac
    16741 cgccagacgg gcgataaaat tgccgacttg ctgttttccc tcaaccgtga gcatggcacc
    16801 acgttgatta tggtgaccca cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg
    16861 ctggtgaacg ggcagttgca ggaggaagca tgattgcacg ttggttctgg cgcgaatggc
    16921 gctcgccgtc gctattaatt gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg
    16981 cgctgggcaa tatcagcgat cgcatggaga agggcttaag ccagcaaagc cgggagttta
    17041 tggcgggcga tcgggcgttg cgcagttcgc gcgaagtgcc gcaagcgtgg ctggaggaag
    17101 cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt cgccaccatg acatttgcag
    17161 gcgacacacc gcagctggcg aacgtcaaag cggtggatga tatctatccg atgtatggcg
    17221 atctgcaaac caatccccct ggcctgaaac cgcaggcggg cagcgtattg ctggccccac
    17281 gcctgatggc actacttaac ctgaaaacgg gcgacaccat tgacgtgggc gatgccacct
    17341 tgcggattgc cggagaagtg attcaggaac cggattccgg ttttaacccc ttccagatgg
    17401 ctccgcgtct gatgatgaat ctggcggatg tcgataaaac cggagccgtg cagccgggga
    17461 gtcgggtcac ctggcgttat aaattcggcg gcaacgagaa ccagctcgac ggctatgaga
    17521 aatggttgtt accccagctt aaacccgaac aacgctggta cggtctggaa caggacgaag
    17581 gcgcgctggg gcgatcgatg gaacgctcgc aacagttcct gctgctttcg gcgcttctga
    17641 ccttgctgct ggcagtggca gcggtggcgg tagcgatgaa tcattactgt cgcagtcgct
    17701 acgatctggt ggcgatcctc aaaacgctgg gggcagggcg agcgcaactg cgtaagctaa
    17761 tcgtcggtca gtggttgatg gtgctgacgc tttcagccgt taccggtggg accataggcc
    17821 tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt tctgcctgct gcactaccgc
    17881 cagccagcct ctggccgtgg ctgtgggcgc ttggcaccat gacggtcatc tcgctgctgg
    17941 tggggctacg accataccgc ttgttgctgg caacgcagcc tttacgcgta ttacgtaatg
    18001 atgtggtagc gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt gtggtggttg
    18061 tgctgctgct cgccggatta atgggtggca gcatgctgct ttgggcggtg ctggcgggcg
    18121 cggtagtact ggctttgctg tgcggtgtgc tgggctggat gcagctgaat gtacttcgcc
    18181 gcatgacgct gaaatcgcta cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt
    18241 ggtcaacgtt aagccagctt tcggcatttt cgctctcctt tatgctgctg gcgctgttgc
    18301 tggtattgcg tggcgatctg ctcgaccgct ggcagcagca gctaccgcca gaaagcccga
    18361 actacttttt aattaacatc gccacagaac aggtagcacc gctaaaagcg ttcctcgcgg
    18421 aacatcatat agtcccggaa tcattttatc cggtagtgcg ggcgcggctg acggcgatta
    18481 acgataagcc gacagaaggt aatgaagatg aggcgcttaa tcgcgaactc aatcttacct
    18541 ggcaaaatac gcggcccgat cataatccga ttgtcgccgg taactggccg ccaaaagccg
    18601 atgaagtgtc gatggaagag gggctggcga aacgcttaaa cgttgccctc ggtgataccg
    18661 tgacttttat gggcgatacc caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg
    18721 actgggaaag tctgcggcct aatttctatt ttattttccc tgaaggggca ttagacgggc
    18781 aaccgcagag ctggcttacc agtttccgct gggagaatgg caacggcatg ttgacacaac
    18841 tcaaccgcca gttcccgacc attagcctgt tagatattgg cgcgatttta aaacaggtcg
    18901 gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg gtcaccgcct
    18961 gcggtatgtt gctgttgctg gcacaggtgc aggtgggtat gcgtcagcgc caccaggagc
    19021 tggtggtgtg gcgcacactc ggtgcgggga aaaaactgct gcgtaccacg ttgtggtgtg
    19081 agttcgccat gcttgggttt gtttccggcc tggtggccgc aattggtgcg gaaacggcgc
    19141 tggcagtgtt gcaggcgaaa gtgtttgatt ttccgtggga gccagactgg cgattgtgga
    19201 ttgttctgcc gtgcagcgga gcgctgctgc tgtcgctttt cggcggctgg ctgggtgcgc
    19261 gactggttaa gggtaaggcg ctgttcaggc agtttgcggg gtgatgagag taaataaggg
    19321 gcgttctggt tgaatggaac gccttgttta aattaattct
//
LOCUS       DAMZII010000092        19018 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZII010000092 DAMZII010000000
VERSION     DAMZII010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.092"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(138..1007)
                     /locus_tag="POA77_003177"
     CDS             complement(138..1007)
                     /locus_tag="POA77_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDI6366564.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="POA77_003178"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="POA77_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDI6366565.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="POA77_003179"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="POA77_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI6366566.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLAQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="POA77_003180"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="POA77_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDI6366567.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="POA77_003181"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="POA77_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDI6366568.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="POA77_003182"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="POA77_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDI6366569.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="POA77_003183"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="POA77_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDI6366570.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="POA77_003184"
     CDS             complement(4585..5718)
                     /locus_tag="POA77_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI6366571.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="POA77_003185"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="POA77_003185"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDI6366572.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="POA77_003186"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="POA77_003186"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDI6366573.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="POA77_003187"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="POA77_003187"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDI6366574.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="POA77_003188"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="POA77_003188"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDI6366575.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="POA77_003189"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="POA77_003189"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDI6366576.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="POA77_003190"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="POA77_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDI6366577.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(12266..15589)
                     /gene="mscM"
                     /locus_tag="POA77_003191"
     CDS             complement(12266..15589)
                     /gene="mscM"
                     /locus_tag="POA77_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDI6366578.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(15611..16579)
                     /gene="psd"
                     /locus_tag="POA77_003192"
     CDS             complement(15611..16579)
                     /gene="psd"
                     /locus_tag="POA77_003192"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDI6366579.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16676..17728)
                     /gene="rsgA"
                     /locus_tag="POA77_003193"
     CDS             complement(16676..17728)
                     /gene="rsgA"
                     /locus_tag="POA77_003193"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDI6366580.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIQEAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17823..18368
                     /gene="orn"
                     /locus_tag="POA77_003194"
     CDS             17823..18368
                     /gene="orn"
                     /locus_tag="POA77_003194"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDI6366581.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18579..18654
                     /locus_tag="POA77_003195"
     tRNA            18579..18654
                     /locus_tag="POA77_003195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18612..18614,aa:Gly,seq:gcc)
     gene            18691..18766
                     /locus_tag="POA77_003196"
     tRNA            18691..18766
                     /locus_tag="POA77_003196"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18724..18726,aa:Gly,seq:gcc)
     gene            18802..18877
                     /locus_tag="POA77_003197"
     tRNA            18802..18877
                     /locus_tag="POA77_003197"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18835..18837,aa:Gly,seq:gcc)
BASE COUNT         4423 a         4865 c         4939 g         4791 t
ORIGIN      
        1 caggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg ttggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttctta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttggcta tataaagagt aacaacagca atgtatatga ataattaaag
     1201 gttatgcaac gggcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgcc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gctcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttaca gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcgggc
     4981 catatcttca atggtcgact tcacaccata agcttcagca tctaacgccc ctggcgaaac
     5041 atgcactgcc ttaccttcgc gatatcccca ggcgtaattc ttttcttctg ggggaggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgt aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacacgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccatagcca gacccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt ggagcccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 aggccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggtg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgcc ggactggagc ctacgcaggt ggttggtctg ctggcagtag catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccgggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 cgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ctggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagtc gcatggcgaa taaacctgag ttagctctcg actgatcata
    12121 tgaaatcctg caactcatcg tctctccggc gatgagttgt caggaagtaa tgcgtatgga
    12181 tgatactctc cagggccggt gataactgaa gatgatgtta agctgctaaa tgagccacgt
    12241 tgacatcatt tggcagcttc atgcattaca aacttcccgc ctgacgacct ttgcccgcag
    12301 aagttaatgt tctccccgtt tgtttaccat tcaggctttc cagacgcatc tggaagggcg
    12361 ggaatggcat atcgataccg tgggcatgga agcctgccag aatcagctgg tggatctcat
    12421 ggcgtagcgg catacggtga cccatctcag cggcgtaaat acgcagctcg aaaatctgaa
    12481 tcccttgttg cagatccacc aggaagactt ccggtgccgg gttgtcgatc accagcgagc
    12541 agcgacgcgc tgcggtgagc aggatttccg tcacttcttc actgttggca tcggcagggg
    12601 ccggaatcgt caacaccacg cgcgtgaccg agtcagagag cgaccagttg ataaactgct
    12661 cggtaataaa cgccttgttc ggcacgatta tctctttacg gtcccagtcg ctgatggtgg
    12721 ttgcgcgggt attaattttc gtcacgctac cagtaagatc gcggatcgtt accgtatcgc
    12781 caatgcgaat cggtttttcg aacaggatga tcaggccaga gataaagttg gcgaaaattt
    12841 cctgtaaacc aaaaccgaga ccaacaccga gcgcggcaac cagccactgc aatttcgacc
    12901 actcaatacc aatcattgag aagccgacca gcccgccaat cagcatcagc agatatttgg
    12961 tgatggtggt gatggcgtaa cccgtacccg gcgttaaatc caggtgctgc aaaatcgcca
    13021 gttccagcag cgcgggcagg ttgcgcacca gctgcgtggt gatgataaac accagaatgg
    13081 caatcagcac cgcaccgagg gtaattggct ccagactttc tacgccctgt accgtggagg
    13141 tgacatccca cagcgaaata ttttcgagga agccgaaagc ggaatggatt tctgaccaca
    13201 gcacaatgac cgaaagcagg gcgatcaaca tcaaaattga gcgcaccagc cgcaaggatt
    13261 gcgcactgat ggcatcgaga tcgacttcgc tttcatcgac ttcaattgcc ccttccgggc
    13321 tactgtgatg atgtgcttcc tcttcgccac gcgcacgttg cgctaacatc tctgcccggc
    13381 gatgcttcgc ccggtcaaac gccagcctgc ggcgttggat cagcatccag cggcggataa
    13441 cgtgataaac caccagtagc aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca
    13501 gcgcctgtgc cgttgccaga taacccaccg ccgacgccag aatggcaacc agtggcgcgc
    13561 caatcatcat gttccacagc atatggttgg taatgttgtc gccgctgccc tctttgttga
    13621 gatacagtgg gatcccggcc tttttcaggc tgagggtgac caccgccagc gcaccgcaaa
    13681 tgaggataaa gcaaagccgt cccagcgaac cggagaattc acggtcgtcg aggttatcga
    13741 acatcatcag cgccataatc agcggcacaa taagcccgat gctcatcagg tagtagcgca
    13801 tcccacggga aacacgttcg cgcggccagc caaaatgagc gataaacaag ccgttcgggc
    13861 gggcaaaggt ggcgcaaatc atcactaccc acagcagcgg cacggtggcc gttacaccat
    13921 cgccaatcgc gaccgccagc ggataaggcc acgcctcgcg caagccgtaa cccagcgtca
    13981 tccacagcac cggcaacggt gacgcgacga gaatcgacca gaaaagagtg cgtaacgtca
    14041 gccagaagtg atcctgagtc actttgccga ctttcgccgc cgaacgttca agaaaacggg
    14101 tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat caacgcgcca aacagcggca
    14161 aaattgtctc tttgctggtc agcatcatca cactggcttt gcccaactga ctgaaggtgt
    14221 ccagcgaaat gagacgacgc agatcctggg cgatttccag cggccaggcg atggtcatcg
    14281 ggcgcacgtc agaggtccag aacagatagc ggtgcgttgc ttcgttcacc tctttcagcg
    14341 catcctccag ttgcccgttg gagactttca gcttggtcag ttccaatagt agcgtgtcgc
    14401 caccctgcaa caatgagttc agcaactcac gctgagtgcg tagttgtgct tccagaatac
    14461 ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg aatttgccgt agcagcggct
    14521 gtttattaag cagatcctca taacgtaacc gttgcacacg caactgcgcc atttcggtat
    14581 caagctgttg tggtttcggc atttccggca gccgtgccac ctgcgcccgc aacgcttcgc
    14641 cgagcaggtt ggacgatccc agccattgcg actgttcacg cagcgtattc agcgcctgcc
    14701 gaacctgtaa cgtctggctg gtggcctggc gctgttgcga ggcaacgaga tccatccgct
    14761 gcgcctgttg attcagtgcc gccgatagtt cgcggttaat tttgaattgc gcgacgatgt
    14821 ctttcggcaa atcggcgctg ttttctgcca gcaattcggt actttccagc gcccgctccg
    14881 cctcaagctg acgttggctg ttcaactgat tacgtaaggc ctgcaaatac gcatccagtt
    14941 gctggctctc tttttccgcc agctctgagc gtaagcgcgc taattcctga cggttattgg
    15001 cagacagctg cgccagttcc agttcatcaa cgagcgcttt caggcgtgcg gagtcagact
    15061 gcaacgcgaa attttgtgcc tgattgagcg gggtattgcc agtaagcgtt cccaggcggc
    15121 gctcgatctc atttaactga cggcgggcgt cggtttgctg ttgcggcagt tgattcagcg
    15181 aatcggcaat ctcgcgggcg cgctcctgct cttgctgggc ctgacggctt ttatccagca
    15241 actggctgct gacctggaga atttcctgat tcagcgcgtc ggtagacatc cccggcgaca
    15301 cgctgcgcgg ttcgtcacgc atgttgttta attgtgcgcg cagagtagcg gaaagtttcg
    15361 ggtaattatc aatgacttct tgatattgct tgatgcgctc aagggaacct tttcgttcct
    15421 caagcgcatt taaggcggac tggagcgcct ctacgacttc cggctgcgcg ggtttcgccg
    15481 cttttgcctg ctccagttct tgtgagattt gtttgctatc gggggccgtc gcggcgtacg
    15541 ccccccaact gaggcaccag gccatcagaa aagtgataat caggcgcacg tcagcgtttc
    15601 ctttgatgga ttagacctgg tcttttttat cgtcaaccaa tgggctggcg tcgtgttctg
    15661 cttcgatctc ttcagcagga agcggggccg gttcagcgtc tggcgtaaca aaggtttcgg
    15721 tagatactgc cagcggctgg ccaattttcg tgacagacag gctttccagt tgctcaacca
    15781 gattcacttt acccggtgca aacaggttga taacggtgga accgagttta aagcgaccca
    15841 tttcctggcc tttcagcagt gccacagaac cgtcgttttc cccggcaggc caggtccagc
    15901 gcttgatgat accttcgcgc ggcggcgtaa tggtgcccgc ccagaccgtc tcaatgctgc
    15961 caacaatcgt cgctccgacc agaatctgcg ccattgggcc aaattcggta tcgaaaaggc
    16021 aaatcacgcg ttcgttacgg gcaaacagat tcggcacgtt ctgagccgtg agatggttaa
    16081 cggagaagag atcgcccggt acgtagatca tctcacgcag aataccgttg cacggcatgt
    16141 gtacgcgatg gtagtcacgc ggggagaggt aagtggtcac aaacgtaccg ttgcggaaca
    16201 ggtccgccat cagatagttg cctgccagca gggcttcgag gctgtagttg tggcctttgg
    16261 cttgcaggat tttatcttct tcgattttac ccaactggct gataacgcca tcggcaggca
    16321 tgaccaggac attcggatcg gtatcgattg ggcgtacttc gtcacgcagc ggacggacaa
    16381 agaattcgtt aaaggtgcgg tagctggcgg tgtccggctt ttgcgcctct ttcatgtcga
    16441 ccttgtagta tttaacgaac agatcgataa ccagttttgt cagccatcct gcccgcttgc
    16501 ttgcgcccca acccgccagg cgagtaagcc atagtttcgg cagaatgtac tgtagcgaaa
    16561 gtttaaatga atttaacaag gtagcctcca ggccattgtt ttgtcgttcc tgatccggcc
    16621 tacatgccgg atcctgaaaa aaaggggacg attctaacga cggttagctt aattgtcagt
    16681 catccgtatc agaaaagttt ttacgcgttt ttacctgcgc catgctttcc agaatacggt
    16741 gatagttttc gaaacgggtt tccgcgattt tcccttcctc aaccgcttcc tggatagcgc
    16801 agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca cagacctaaa tagtcatgga
    16861 attcgacaaa gccctgagtg atttgttccg gctccaggtg ccagaggccg aactcacgca
    16921 caccagggga atcaatcaca tcaccgccgt gcgggaagtg atacagccga gcggcggtcg
    16981 tggtgtgctg accgagcccc gagttgtcag agacatcgtt ggtcaggatc tctttttgca
    17041 gccccagcaa cgcattcagc aggctggatt tgccgacgcc agactgcccg gcaaaaatgc
    17101 tgatgcgccc ggtcaacgcc tcttccagcg gttttagccc atcctgagta tggctggaaa
    17161 ccatcaatac gcgataaccg atattgcggt agatatccat ctgctcgttg acgaacgcca
    17221 tgccttcgtc gtccagcagg tctatcttgt tgagcacaat aatcggctca atctgcaagg
    17281 tttcgcaggc caccaggtaa cggtcgataa tattgagcga cagctccggc aaaatggcgg
    17341 agacaatgac aatctggtcg atgttggcgg caataggttt tacgccgtcg tagaaatccg
    17401 gacgcgtcaa caccgaggta cgctcatgca ccgcttccac gatccctttg acatttacgc
    17461 cttccgccgc cggtttaccc ggacgccaga ctacgcggtc gccggttacc agcgaacgga
    17521 tggtacggcg aatattgcag cggtgaacgt cgccatcggc ggattccaca tcagcgtgca
    17581 taccaaagcg gctgatgacg ataccttcat caggctcgcc aaacagattg tcgtcgtagt
    17641 cgggcttctc cttagacgtt ttaagacgac gctggtgatt ggcgttcacg cggcgctgct
    17701 ggcctttgga gagtttattt ttactcaatc gtacagactc ctggtcgccc ctgatgggca
    17761 aaacatctat gatacacgca attgtggatc aatatagtca ctgtgaatgg gtggaaaata
    17821 gcatgagtgc caatgaaaac aacctgattt ggatcgatct tgagatgacc ggtctggatc
    17881 ccgagcgcga tcgcattatt gagattgcca cgctggtgac cgatgccaac ctgaatattc
    17941 tggcagaagg gccgaccatt gcagtacacc agtctgatga acagctggcg ctgatggatg
    18001 actggaacgt gcgcacccat accgccagcg ggctggtaga gcgcgtgaaa gcgagcacga
    18061 tgggcgatcg ggaagctgaa ctggcaacgc tcgaattttt aaaacagtgg gtgcctgcgg
    18121 gaaaatcgcc gatttgcggt aacagcatcg gtcaggaccg tcgtttcctg tttaaataca
    18181 tgccggagct ggaagcctac ttccactacc gttatctcga tgtcagcacc ctgaaagagc
    18241 tggcgcgccg ctggaagccg gaaattctgg atggttttac caagcagggg acgcatcagg
    18301 cgatggatga tatccgtgaa tcggtggcgg agctggctta ctaccgcgag cattttatca
    18361 agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc acttgcggcg gcgtgatttt
    18421 gacgctaaat tgaacgtctt gctgataatt tcagcacttg agataaaaac gcaaaaaaaa
    18481 cttttttggg gggttgcaga gggaaagatt tctcgtataa tgcgcctccc gtaacgacgc
    18541 agaaatgcga aaattacgaa agcaaaatta agtagtacgc gggaatagct cagttggtag
    18601 agcacgacct tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc tccaaaattt
    18661 gaaaagtgct gcaaagcaca gaccacccaa gcgggaatag ctcagttggt agagcacgac
    18721 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaaat ttgaaagtgc
    18781 tgtaaggcac agaccaccca agcgggaata gctcagttgg tagagcacga ccttgccaag
    18841 gtcggggtcg cgagttcgag tctcgtttcc cgctccaaat tcttctctca ataaaatatc
    18901 cacagcgatg cgatgcgtta ttgctggttt tcgttgtctc tgacaaactc ttgtaaacag
    18961 agttatccac agcctcaggc tgtaatctta atttcaaaga aacttcgcac ggtgaata
//
LOCUS       DAMZII010000093        18620 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZII010000093 DAMZII010000000
VERSION     DAMZII010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.093"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="POA77_003198"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="POA77_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDI6366582.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="POA77_003199"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="POA77_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI6366583.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRITAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="POA77_003200"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="POA77_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDI6366584.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="POA77_003201"
     CDS             complement(1087..1581)
                     /locus_tag="POA77_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDI6366585.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="POA77_003202"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="POA77_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDI6366586.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="POA77_003203"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="POA77_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDI6366587.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="POA77_003204"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="POA77_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDI6366588.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPPGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="POA77_003205"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="POA77_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDI6366589.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="POA77_003206"
     CDS             complement(6745..7032)
                     /locus_tag="POA77_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDI6366590.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="POA77_003207"
     CDS             complement(7151..8038)
                     /locus_tag="POA77_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI6366591.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="POA77_003208"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="POA77_003208"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDI6366592.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="POA77_003209"
     CDS             complement(9275..9769)
                     /locus_tag="POA77_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDI6366593.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="POA77_003210"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="POA77_003210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI6366594.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="POA77_003211"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="POA77_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDI6366595.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="POA77_003212"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="POA77_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI6366596.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="POA77_003213"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="POA77_003213"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDI6366597.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="POA77_003214"
     CDS             13861..14520
                     /locus_tag="POA77_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6366598.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="POA77_003215"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="POA77_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDI6366599.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="POA77_003216"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="POA77_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI6366600.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="POA77_003217"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="POA77_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDI6366601.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..>18620
                     /locus_tag="POA77_003218"
     CDS             17948..>18620
                     /locus_tag="POA77_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDI6366602.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQF"
BASE COUNT         4641 a         4976 c         4658 g         4345 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgatgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc ctggcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtagcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta
//
LOCUS       DAMZII010000094        18549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZII010000094 DAMZII010000000
VERSION     DAMZII010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.094"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..1332
                     /locus_tag="POA77_003219"
     CDS             112..1332
                     /locus_tag="POA77_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI6366603.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1305..1934
                     /locus_tag="POA77_003220"
     CDS             1305..1934
                     /locus_tag="POA77_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDI6366604.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQKLFADRQYSRAWTVK"
     gene            complement(1935..3095)
                     /gene="ybdL"
                     /locus_tag="POA77_003221"
     CDS             complement(1935..3095)
                     /gene="ybdL"
                     /locus_tag="POA77_003221"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDI6366605.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3204..4292
                     /gene="hcxA"
                     /locus_tag="POA77_003222"
     CDS             3204..4292
                     /gene="hcxA"
                     /locus_tag="POA77_003222"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDI6366606.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLSQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4302..4499)
                     /locus_tag="POA77_003223"
     CDS             complement(4302..4499)
                     /locus_tag="POA77_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI6366607.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4681..6786)
                     /gene="cstA"
                     /locus_tag="POA77_003224"
     CDS             complement(4681..6786)
                     /gene="cstA"
                     /locus_tag="POA77_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDI6366608.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6967..7380)
                     /gene="entH"
                     /locus_tag="POA77_003225"
     CDS             complement(6967..7380)
                     /gene="entH"
                     /locus_tag="POA77_003225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDI6366609.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7383..8129)
                     /gene="entA"
                     /locus_tag="POA77_003226"
     CDS             complement(7383..8129)
                     /gene="entA"
                     /locus_tag="POA77_003226"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDI6366610.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8129..8986)
                     /gene="entB"
                     /locus_tag="POA77_003227"
     CDS             complement(8129..8986)
                     /gene="entB"
                     /locus_tag="POA77_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDI6366611.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9000..10610)
                     /gene="entE"
                     /locus_tag="POA77_003228"
     CDS             complement(9000..10610)
                     /gene="entE"
                     /locus_tag="POA77_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDI6366612.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLSPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     SEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10620..11795)
                     /gene="entC"
                     /locus_tag="POA77_003229"
     CDS             complement(10620..11795)
                     /gene="entC"
                     /locus_tag="POA77_003229"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDI6366613.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12170..13126
                     /gene="fepB"
                     /locus_tag="POA77_003230"
     CDS             12170..13126
                     /gene="fepB"
                     /locus_tag="POA77_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6366614.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13130..14380)
                     /gene="entS"
                     /locus_tag="POA77_003231"
     CDS             complement(13130..14380)
                     /gene="entS"
                     /locus_tag="POA77_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDI6366615.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14491..15495
                     /gene="fepD"
                     /locus_tag="POA77_003232"
     CDS             14491..15495
                     /gene="fepD"
                     /locus_tag="POA77_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDI6366616.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15492..16484
                     /gene="fepG"
                     /locus_tag="POA77_003233"
     CDS             15492..16484
                     /gene="fepG"
                     /locus_tag="POA77_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDI6366617.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16481..17296
                     /gene="fepC"
                     /locus_tag="POA77_003234"
     CDS             16481..17296
                     /gene="fepC"
                     /locus_tag="POA77_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI6366618.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17293..18426)
                     /gene="fepE"
                     /locus_tag="POA77_003235"
     CDS             complement(17293..18426)
                     /gene="fepE"
                     /locus_tag="POA77_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDI6366619.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4354 a         5255 c         4791 g         4149 t
ORIGIN      
        1 atttattcac agcgcgaatg aaaaataaaa cactctgctt tacagtgttt actttataat
       61 taacagtctt atctcactgt tcaccctgta acgttcagag agcgtcttca gatgtctatt
      121 tataaaattc ctcttccgct caatattctg gaagctgcac gagagcgtat tacctggaca
      181 ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg gcaaagactc tggtttaatg
      241 ctgcatctga cagccgaact tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc
      301 gactgggagg cgcaattctc ttgcaccatt aactatgttc agtccctgcg cgagttgtac
      361 accgatgtca tcgaagagtt ttactgggtt gcgctcccgc ttacgacgca aaattccctt
      421 tcacaatacc aacccgaatg gcagtgctgg gaacctgatg tcgaatgggt gcgtcagccc
      481 ccgcaagatg cgataaccga tcctgacttt ttctgctttt accagccagg catgaccttc
      541 gaacaatttg tacgtgagtt tgccgaatgg ttttcacaaa aacgtccggc ggcgatgatg
      601 atcggcatcc gtgcggatga gtcctacaac cgttttgtcg ccatcgccag tttaaataaa
      661 caacgttttg ccgacgataa accctggact acggccgcac caggcggtca tagctggtac
      721 atttacccca tttacgactg gaaagtggct gatatctgga cctggtatgc aaatcatcaa
      781 agtctctgca acccactgta taacctaatg tatcaggcag gcgttcctct gcgtcatatg
      841 cgaatttgcg aaccttttgg cccggagcaa cgacaaggat tatggctcta tcacgttatc
      901 gaaccggatc gctgggctgc tatgtgcgca cgagtcagcg gggtaaaaag tggcggcatt
      961 tacgccggac atgacaatca tttctatggg catcggaaaa tcctcaagcc agaacattta
     1021 gactggcaag aatatgcatt attgctgctc aatagcatgc cggaaaaaac agctgagcat
     1081 taccgcaata aaatcgccat ttatttgcac tggtatcaga aaaaaggcat cgaggttcca
     1141 caaacccagc aaggggacat tggcgcgaaa gatatcccct cctggcggcg gatatgcaaa
     1201 gttttactca ataacgatta ctggtgtcgg gcattatcat ttagtcctac gaaagcgaag
     1261 aactatcagc gttataacga acggataaaa ggaaaacgtc aggaatgggg gatactatgc
     1321 aacaacgatt aacgcaggat ttaacgcaat ttctcgccag cttgccggaa gatgatcgca
     1381 tcaacgcgat taatgaaatc cgcatggcga tccatcaggt tagccccttt cgtgaagagc
     1441 cagtggattg cgttctgtgg gttaaaaaca gccaacttat gcctaatgat tacaacccaa
     1501 acaacgtggc accacccgaa aaaaaactgc tgaaaaaatc cattgaaata gatggtttta
     1561 cccaacccat tgtggtcacg catacggata aaaatgccct ggaaatcgtg gatggttttc
     1621 accgccatga aattggtaaa ggctcatcat cattaaaatt acgcttaaaa ggttatttgc
     1681 cggtgacctg tctggaaggt acgcgtaatc aacgcatcgc cgcgactatt cgtcataatc
     1741 gcgctcgtgg acgccatcaa atcaccgcca tgtcggaaat cgtccgtgaa cttagccagt
     1801 tgggatggga cgataataaa attggcaaag agctgggaat ggacagcgac gaagtgctgc
     1861 gcctgaagca aattaacggt ctgcaaaagc tgtttgccga ccgtcaatat tcccgcgcct
     1921 ggactgtcaa ataactacag ctggcgcagt cgttcagctg ctgccagcaa cgtcgattcc
     1981 ttcttggcaa aacagagacg aatcagttta tgagggaagg gatcggcgca aaacaccgat
     2041 agcggaatcg ccgctacgcc gtgctcctgc gtcagccact ggcaaaactc aacatcatcc
     2101 agggtagata cggcgctgta atccaccagc aaaaagtatg taccttcaca cggtaaaatc
     2161 tccagccggc tttcatttaa agcattcacc agaatatcgc gcttctggcg ataaaagtcc
     2221 ggtaacgcaa gataatgctc aggttctgca cgtagcatat cagcaagcgc cagctgtgcc
     2281 ggggtattca ccgaaaaggt cagatactga tgtaccttgc gaatttcggc gctgatgggc
     2341 gctggcgcaa cacaataacc cactttccag ccggtcatat gataggtctt gccaaatgaa
     2401 gaaaccgcca ctgcccgctc acgcagctgc ggatgcgcca gcacactggc atggccctgt
     2461 tgtgaaaagt tgatgtgctc gtagacttca tcgctaatga caaaaatctc gtgcccggcg
     2521 atcgcctgcc acaaagcggc gaaatcagcc tgctgccaga cagttgcact ggggttatgc
     2581 ggagtgttga ggatcaccag tctggtgcgc tcgcttaaca atgcggcaaa ttcctgccag
     2641 tcaacgcgaa aatgcggtgg ttgcagtgcc atacgcttca ctattccccc agaaagcgcg
     2701 atggcggggg agtaactgtc atagctggga tcaaaacaaa tcacttcatc gccattgcgc
     2761 accagtgcgg taatcgccgc gtataacgct tccgtcgccc ctgccgttac ggtgatatcg
     2821 ctatcggcat ctggttgata gccatacaaa cgttccgttt tctgagcaat cgcctcgcgc
     2881 aaggcctgca cgccggtcat gggcgcgtat tggtttgccc cctgggcaac gtggtacgcc
     2941 agccgctcct gtaaatagcg cggaccatca aaatcaggaa agccttgcga caggttaatc
     3001 gcctggtgtt gctgcgccag cgcgctcatc tgggtgaaaa tagtggtgcc aagttgtgga
     3061 agtttgcttt gtggaatcag agggttattt gtcattttta ttgtacctga cgaagttgcg
     3121 tgttgaaggc actataacac gatgctagta tttggcaatc aagacgttta gatgtctaaa
     3181 taaaacaata aggacaacac aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg
     3241 ctaactactt ttcacatcca ggaagtttca atcacctgca cgattttttc actgatgaac
     3301 aactttctcg cgcggtgtgg atctacggca aacgcgccat tgctgcggcg caaaccaaac
     3361 ttccgccagc gtttggactt ccaggggcaa agcatatttt gtttcgtggt cattgcagcg
     3421 aaagcgatgt acagcaactg gcggctgagt ccggtgacga tcgcagcgtg gtgattggcg
     3481 tcggcggcgg cgcactgctc gacaccgcga aagccctcgc ccgccgtctc ggtctgccat
     3541 ttgttgccgc tccgacgatc gccgccacct gcgcagcctg gacaccgctc tccgtctggt
     3601 ataacgatgc cggacaggcg ctgcattatg agattttcga cgacgccaat tttatggtgc
     3661 tggtggaacc ggagattatc ctcaatgcac cgcaacaata tctgctggcg gggatcggtg
     3721 acacgctggc gaaatggtat gaagcggtgg tgctggctcc gcaaccagaa acgttgccgc
     3781 taaccgtgcg actggggatc aataatgcgc aggccattcg cgacgtattg ttaaacagta
     3841 gcgaacaggc gctgagcgat cagcaaaatc aacagttaac gcaatcattt tgcgatgtgg
     3901 tggatgctat tattgctggt ggtgggatgg tcggtggtct gggcgatcgt tttacgcgtg
     3961 tggcggcggc tcatgccgtg cataacggtc tgactgtcct gccgcaaacc gagaaatttc
     4021 tccacggcac caaagtcgct tatggcattc tggtacaaag cgccttgctg agtcaggatg
     4081 atgtgctggc gcaattaacc ggggcgtatc agcgttttca tctgccgacc acgctggcgg
     4141 agctggaagt ggatatcaat aatcatgcgg agatcgacaa agtgattacc cacaccctgc
     4201 gtccggtgga atccattcat tacctgccag tcacgctgac accagatacg ttgcgtgcag
     4261 cgttcgaaaa agtggaatcg tttaaagcct gacgtgcaaa tttagcagca gcgcgcgccg
     4321 ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa actcttcgta ggtcatcggc
     4381 gtttgatcgg gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca
     4441 atcatcagct tcgccgcctg acctaaatat tttccggctt ttgccagtga atcaaacatc
     4501 ttggcctccg ctaaccatgt tgctgaaaga gtgccggatg cggcgtgaac gccttatccg
     4561 gcctacaaat gctatcaacg cgctcggtca gtcccctcgc cccatcgggg agagggttag
     4621 ggtgaggagg aaccctcggc actatgccaa cattcgccaa catcccccct cactctgact
     4681 ttagtgtgcg ccttttgcct gcgccacgat ctcctcgaca ttttccggca ttggctcata
     4741 cggcgtttct ttcgccgttg gcttcggatc tttcaatgcc gccagtgccg tcttaatcga
     4801 gaacagcgcc agaaccacca cgaccaccat aaagaagatg gttaacccgg catccagacg
     4861 gttgttgaac accagttgcg ccagctgtga ctcagtatac tgcgacggaa tattgccgct
     4921 gtcgatcatt gcctggaact tattagcaat ggccaggaag ccgactttcg catccgggct
     4981 aaacgctttc tgccagcctg cggtcagggt acaaatcagc agccaggccg ttggtaccag
     5041 cgccacccag gcgtaacgtt gacgtttcat cttgaacaac accacggcac agagcatcag
     5101 cgccatccct gccagcatct ggttggcaat accaaacagc ggccacagag tgttaatgcc
     5161 gcccagcgga tcgaccacgc cctgatggag gaagtagccc cacgccagta cgcacagcgc
     5221 tgttgccagc aggttagcag gcagtgaatc ggtccgcttc aggcccggag aaaccacgcc
     5281 cagcagatcc tgcaacataa agcgcgcagc acgcgtaccc gcatctaccg ccgtcagaat
     5341 aaacagagct tcaaacagaa tggcgaagtg ataccagaac gccacatcca tcataccacc
     5401 cagcgcaccg tgcaggatgt aagccatccc caccgccagc gtcggcgcac cgcccgcacg
     5461 ggaaatgatc gactgttcac ccacttcgct ggcaatctgg ttgagcgtat ctggtgtaat
     5521 gctaaagccc cagctactca ccacctgcgc ggcagaagcg actacatccg ccgtccctgc
     5581 cggagccagc accgccattg ggctgttcat ggcaaaatac acgcccggat cgatgataca
     5641 ggcagaaacc agcgccataa tcgccacgaa ggattccatc aacatcccac cgtagccgat
     5701 aaagcacgcc tgcccttcgt tcgccagcat cttcggcgtg gtgccggaag agatcagcgc
     5761 atggaagcca gacaccgcac cacaggcgat ggtgataaac aggaacggga acaggttacc
     5821 ggtccatacc gggccagtgc catcgacaaa tttggtcagc gcaggcatgg tcagcgtcgg
     5881 gcgcataatc aaaatgccta ccgccagacc gacgatagtc ccgattttca ggaaggtcga
     5941 gagatagtca cgcggggcca gcagcaacca caccggcagc accgccgcca caaaaccgta
     6001 gcccaccagc atccaggtca gctgcacgcc ggtaaagtca aagtacggtg cccaggtcgg
     6061 actttctgcc acccagccgc cagagataat ggcgaaaatc aggaacacca ggccaatcac
     6121 tgacacttcg ccaatgcggc ccggacgcag atagcgcagg tagattccca taaaaatcgc
     6181 cagcggaatg gtgaacgcaa cggtatacgt tccccacggg ctatgagtca gggctttcac
     6241 cacgatcatc gccagtactg caaggataat gaccatgatc ataaagcagg ctaccagcgc
     6301 aatcaccccg gcagttggcc ccatctcttc tttgaccagc tcacccagcg atcgaccgtc
     6361 acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc gcaccagcga gaaccacccc
     6421 ggcgagcagc cagatcatcc ctggcaggta acccatttgc gccgccagta ccggcccaac
     6481 caacggacct gctccggcaa tggccgcaaa atggtgaccg aacagcactt tcttgtccgt
     6541 cggcacatag tccagcccgt cgttatggcg caccgctggc gtcatacgcg tcgggtcaac
     6601 cgccagcaca tttttggcga tatacagccc ataaaaacgg taagcgatca gatagataca
     6661 gaccgacgcc accacaatcc acagcgcgtt gatctgttcc ccacgattta aagcaatgta
     6721 tcccagagca aatgctccca ttacagagag cactgtccag acgaggtatt tccctgattt
     6781 gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt acattttgtt tctataacat
     6841 ctttgtacga tttaacaaca cggtaaccat gtaaatcgga gttgaaacca atatttaact
     6901 taacaatgtt aactcgatca ctccgttaac cgagttttcc tgcaatctca tttaattctg
     6961 tcgggctcat cccaaaactg ccgtacccag ccgacaagtg cagcaacgcc gcccctgttc
     7021 atcgaaaacg acgatttccc agctctgatt ttgccgccca agatgcagcg gctggcagac
     7081 gccgcgtacc ttgccttcag acaccgggcg atgatgggtg gcgttaagtt ccgtacccac
     7141 cacacactgc ccgtcacggg tcatcataaa tccggccatc gatcccagcg tttcagccag
     7201 cgccgccgac gcgccgccat gcagcaggcc aaacggctga tgagtacggg tatcaaccgg
     7261 catttcggct tccagcacat catctcccag acgggtatac acaattccca gatgcgccac
     7321 cattgtgtta tcgctggtgg cgttcagttc gtcgagcgtt aaatggcgtt tccagatcat
     7381 gcttatgccc ccagcgttga gccgccatcg accacaatat cctgtagggt gatatggctg
     7441 gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct cttgtggacg ggcgattttc
     7501 cccagcggaa tgccgagttt aaactgctcg ccaaagccgc gaatacgctg ttcttcggcg
     7561 tcatcgctca cccacagcgt gcgttgcata tcggtgtcgg tggagccagg cgaaaccaca
     7621 ttacagcgca cgccgctacc cgccagttcc agcccgacgc tcaacgccag gcttttcagc
     7681 gccgctttcg atgcgccata agcactcata ccaatacgcg gcgtgtgtgc ggcgtcggac
     7741 gccacagtga caatcgcccc gccccgctga cggcgaaact ggttcatggt ttgctggaac
     7801 aggttaaacg caccgccgac gttaaccgca aaagtctgct gccagtcctc tttactgagc
     7861 tgatcggtcg cgcccatgcg caaaattccc gccgcattga tcagcacgtc cagtcgctcc
     7921 gtttcagcta acagtcgctg acatacctgc gccacctgcc cggcgtcggc aacatccatt
     7981 acttcggtcg caaagggata ttgctcctga gtgaacgctt gatcaaaacc tgtaactttc
     8041 gctcccgcct caacaaacgc cagcgccgtg gcgtagccga tacctttacc tgcgccggtt
     8101 acccagacat ttttaccgct gaaatccatt atttcacctc gcgggagagt agcttccacc
     8161 aggcgtcgat ggtcgggttt ttcgccagca tgacaaagtc gatatcacca tgcactttgc
     8221 gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag accgtagtcg atcaggttgt
     8281 cgtcatcgaa cggttcatcg gactcgtcca gcaacggcag gataacctca cgcagcgccg
     8341 ctttgctggc ggggacaggt gctggcagta attcttcagt catcaccacc cggccagacc
     8401 gcccggccac atatttcagc gacatcaaat gctcgtcacg gctgaaatcg gccagcgcat
     8461 ccgccaccat aaacggttta atatcgcgca taaatgcgtc ggttgcggtg gtcatacagc
     8521 caatgtgggc atataccccg gtaataatca gctggttacg tccactctct ttgagcattt
     8581 gctccagcgg agaacgatga aacgcgctgt agcgccactt caccagcacc gtgtcgtcgg
     8641 catctggcgt caggcgatcc accacctttt gctgttccgg cgaacgggtc aggcccggcc
     8701 cccacatatc attcaacagc gcccgatctt catcgctctg ctctttcggc tgggcggtgt
     8761 aataaaccgg gatattgtgc tgtttgcagt agtcgcgcag cgcagcaata ttcgcgatca
     8821 cctgctccat catcgggcag ttctcgcccc agaagctgac aaaatagtcc tgcatatcat
     8881 ggattaacaa cgcggcacgt tgcggttcaa aggcccagtc aactttattc tgcggaatat
     8941 cgtgagactc cggcagtgcg taagcctgta attttggaat agccatagtg ttctctcctt
     9001 caggctgatg cgcgtgacgc cagccactga cgtaattgtt ttttatcgac tttcccgacc
     9061 gccgtcagcg gaagtgaatc cacacactcc acgcgatccg gtaatttaaa ttcggcaata
     9121 ccctgttcac gcaggaaacg acgcacctgc accgcgcgca gcggctcttt taccaccaga
     9181 taagcgcagc ttttttcgcc catcagctca tcttccatgc tcaccagtgc ggcatagatc
     9241 actgccggat ggcgcagcag caggttttcg atctcttcgg cagcgatctt ctcgccgcca
     9301 cggttgatct gatctttctc gcgcccctgc acggtgatgt aaccctctgg atcaatagag
     9361 atcagatcgc cggaacagta aaaaccgttg gcatcaaagg cgctggcatt gtgctgcgga
     9421 cttttgtaat aaccgcggaa ggtgtacggc ccgcgcgtca tcaggcgtcc gacttcccct
     9481 tgcggcagtg gatttccttc ggcatcggca acccacactt catcgtccgg acacattggg
     9541 taaccctggg tatggataat tttctccgcg ctatcatcaa gacgggtgta gttcaccagc
     9601 ccttccgaca tgccaaacac ctgctgcaac tgacagccaa tctcagcggg aatacgcgcc
     9661 gcaagggtgg cagaaagacg tgcgccgccg acctgtaaca gtttcagcga ggcaagctgc
     9721 gcccggcttt cgccttcggt cagcgcctgc aaccacaggc tgactgcggg tggcaccagc
     9781 gcggtaacgt taacctgatg tttttcaatc aatgggaaac agagcgtggc gctgggatcg
     9841 gccgccagaa caaccgttcc tccggcaaga aagacgccca gcgatcccgg cgaactcatg
     9901 gcgtagttat gagccgccgg gatcgcgcac aggtagcgtg tctgttgtgt gaactgacaa
     9961 atctcgacgc tacgacgcac gctgtagtag tagtcgttat gagtgcgcgg gctcagtttc
    10021 ggtgtgccgg tggtgccgcc ggaaagctgg aaataggcca cttcatcagc aggtgatggc
    10081 gtggcagtaa aatcctcagc cggatggtta atcgcatcct gcaagttatg ctcaccgctg
    10141 tcgttgtgca gttgcaccac gcgaatggag gaatgttctg tgacgaaagt attgaggaaa
    10201 tcatccccgc taaacagcgc atgttggcga tcggcaatca gcaatgcggg ttcaatctgg
    10261 ctggcatagg cgttcagttc actacgctga tggctgaaca gcgccagcac cggcgcaacg
    10321 cccagtttca gcagcgcgaa aaaggtaata tacaattcag cgacgttacc cagttgtacc
    10381 agcgcggttt caccaggttt aatgccctga cggcgtaaac tacacgcgag gttatccgcc
    10441 gcctgattca gctcccggta actcaactgt cgctcgccgt cgataaccgc gatgctgtca
    10501 ctcgcagcgt ggcgagtcag aatgtcggtc agcggcaaat cctgccagta gcctttttcc
    10561 cgatagcgac gggcaaactc ttccggccag cgggtgaatg gaatgctcat cctcgctcct
    10621 taatgcaatc caaaaacgtt caacatggta gaaagtttga cgcctgtttc gcgccactca
    10681 cccaacggtg acgacgcagg cacaatcccc gctccggcaa acagacgcac ctgattttcc
    10741 cgcagcttcg cgcagcggat ggtcaccacc cattcgccgt taccttcgct gtcacaccaa
    10801 cccacaatgc cgccaaacag ttcgcggtcg aatggctcca gttcagcaat aacctgggtc
    10861 gcggcctgat gcgggaaacc gctcagcgcg ggggttggat gcagcagaca ggccagagtc
    10921 agtgcgtttt cttgcgaatt cgctttacct tcaaagggag ttgcgagatg ccacagcgtc
    10981 ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact cactactgcg ttcgcgcagt
    11041 acctctttca tcgcctgagt caccagttca tgttcatggc gatctttttc tgacgccagc
    11101 agacgattac ccgcttcgcg atcgagcact tcatccggct gacgacgcgc ggaaccggct
    11161 aacggaatgg agctaaaacg ctcgccgtct ttacgtagca gcagttccgg gctggccccc
    11221 agcaggacgc caccatcagc cagcgggaca tggaagttgt aactaaccgg gttttgcgca
    11281 atcaaccgtt ccagcaatac gccactatca atggcggcgt cagtggtgat atcaatcaat
    11341 cgtgacaaca ccactttgtc gacctgcggc gtggcggtaa gtgcggcggc gcgagcaacc
    11401 atctgttcaa acgtggtttg ttcaggaatt gcctggcgtt ccaccacgtt cagcgactgg
    11461 ctgcgggtaa aacggcgggc tgaggtctgt ttttcctggc gggagaacga ctgccaggat
    11521 tcgggaatat acagcgacga aggctgacgt ggatcgaagg gaatcgcccc gaccatcacc
    11581 ggatttttga tgccctgcgc tttggcatcg gcaaacagcg cggcgagttt ttgctggaag
    11641 ggactgtcgg gcgaatcccc gttcacagcc ggttcatcga agcgggcgaa acatcctgac
    11701 gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat tgggcgcaag tgttgccatg
    11761 gtctgctgta cttcctcagc cagtgacgta tccatatcat cctccacaaa atgataaagg
    11821 ctttaataat gattatcatt tatattttcg gtcgctaacc taaaagcaaa cgcgcactat
    11881 gtcaactctt gaggtaacgc aatctgcaac tcggggttgc atctggttgt ggttacaatg
    11941 aaaatgagaa gcattattga tggattcgca taagcgcaat gtgatgtcct gcgccgttct
    12001 gccccctctc ccttccaggg tgagggctgg ggtgagggtt aatgttcgca ccagtgctgg
    12061 ctgttcccct caccctaacc ctctccccaa aggggcgagg ggacggattg tgcgctttgt
    12121 cgaatttgtc attacgccct taaccttatt aataacagga agctgatttg tgagactcgc
    12181 cccgctctac cgcaacgccc ttctattaac aggacttttg ctttcaggaa tagccgcagt
    12241 tcaggccgct gactggccgc gtcagattac tgacagccgt ggcacacata cactggaaag
    12301 ccagccgcag cgtattgttt ccaccagcgt caccctgacc ggctcactgc tggcgattga
    12361 tgctccggtg atcgccagcg gcgcgaccac gccgaataac cgcgtcgcgg atgaccaggg
    12421 ctttttacgc cagtggagca aggtggcgaa agaacgcaaa ctgcaacggc tctatatcgg
    12481 cgaaccgagc gccgaagccg ttgccgcgca aatgccggat ctgattttaa ttagcgcaac
    12541 cggcggggat tcggcgctgg cactgtatga tcagctttcc accatcgccc cgacattaat
    12601 catcaattac gacgacaaaa gctggcagtc gctgttaacg cagcttggcg aaattaccgg
    12661 gcatgagaaa caagcggcag agcggattgc acagtttgat aagcaactgg cagcggcgaa
    12721 agagcaaatc aaattaccgc cgcagccggt cactgccatt gtctataccg ccgctgcaca
    12781 cagtgccaat ctctggacgc cggaatcagc acaagggcag atgctggaac aactcggctt
    12841 tacgctggcg aagttgcccg cagggttaaa cgccagccaa agccagggca aacgccatga
    12901 catcattcag cttggtgggg aaaatctggc tgctgggtta aatggcgagt cactattcct
    12961 gttcgccggt gatcagaaag atgccgatgc gatttatgcc aatccactgc tcgcgcacct
    13021 tcccgcagta caaaacaagc aggtttatgc gctgggaacc gagacgttcc gtctggatta
    13081 ctacagcgcc acgcaagtgc tggaacgact taaggcgctg ttttaagcat taactgtcag
    13141 acgctgtcac ctgcggcggc gtctggcgaa aacgtcgcaa ctccaccagc accagcaata
    13201 acaacacgcc gataatcaac aaaccaaaac cgcttgcgct tgcggaagca accggtgtca
    13261 tcatcgcgcc caaaccaccc agcagcgccg cgcctatggc atcgcccgtc acgttctgcg
    13321 ccgtccacaa accgttaatc cgccctaaca tcgcttccgg ggtttgcgtt tgcagcattg
    13381 tatattgcag caacgaacta accgcgctca gccagccgaa cagcgccaga caaaccacgc
    13441 ctaaaatcca catcggcatc aggccaaaca gaccaatggc gaggaacgat cccagcgtgg
    13501 agagcagcat caataaccct ggtcgcgcac tatgtgccag cttcccgctg gttaacgcac
    13561 caatagccgc gccgagcggg atcgccgcgt agagaaaacc aatctgtgcc gctgacatct
    13621 gccagttgtc agccagcgcc ggatacagta cccgcaccgc gctcgccatc gtcaataaac
    13681 cacccagcag cgcaatcccg cccaccagcg ggctggcaag cagaaaacga aatcctgcca
    13741 gtaatgattt caacggatgc tcacgcggct gcggtggcgg tggcaacgcc ggaaggctta
    13801 acaacggtag caaggtaata aacgtgcccg ccgccgccag cccgtagttc caggctacgc
    13861 caccggtcgc cagcaataaa ccgccaatca tgggcgaaat caccgacccc agacgcacgg
    13921 tcaacatggt gatcgccccg gcctgcatta agttttcacg ccctaccagt gctggtgtcg
    13981 ccgccaatag cgccgtaacg ccaagcgatg cgaaaaaacc atcccataaa ccaagtaaat
    14041 agattgccag caatgacggc tccggcagca gtgcattaag gcacagtcca atgaagccaa
    14101 tgccacaggt gccgcgcgcc agcaaaatca cttttttgcg ctcatagcga tccgccagca
    14161 caccgccaac catcaggcca acaaacatcg cgccgccggt cagcgtcacc gaaagcccca
    14221 cctgccaggt agaatgcgtc atcatctgga tctgcaccgg caccgcgacg ccgagcaaac
    14281 ccagagacac aattgagata aaacgagcga ggaatactgc gcgaaacgcc gggtgcgttt
    14341 tcaacaggct gaggttaagc agccaggatt gtttattcat tacaatgcct tgccatcaaa
    14401 tattttaaat acctattctt aggctgcaca tgctaacata tccaaataag atcgataacg
    14461 ataattaatt tcattatcat ggaagttcgt atgtctggtt ctgttgccgt gacacgcgcc
    14521 attgccgtgc ccggattgct gttattactg attatcgcga cggcattaag cctgctcatt
    14581 ggggcaaaat cactccccgc ttccgtagtg ctggaggccc tctccggcac ctgccagagc
    14641 gccgactgca ccatcgtgct cgacgcgcga ctgccgcgca cccttgccgg tttacttgca
    14701 ggcggcgcgc ttggccttgc cggggcgttg atgcaaaccc tcacccgaaa tccacttgcc
    14761 gaccccggct tgcttggcgt gaatgccgga gccagctttg ccattgtgct gggtgcggcg
    14821 ctgtttggtt actcatccgc gcaggaacaa ctggcgatgg ccttcgccgg ggcgctggtg
    14881 gcctcattga ttgttgcctt taccggcagt cagggcggcg ggcagttaag tccggtgcgt
    14941 ttaaccctgg cgggcgtggc gctggcggcg gtgctggaag ggctgaccag cggcatcgcc
    15001 ctgcttaatc ctgatgtcta cgatcaactg cgtttctggc aagccggttc gctggatatt
    15061 cgcaatctac ataccttaaa agtggtgctg atcccggtgc tgatcgccgg agcaaccgcg
    15121 ctattactga gccgcgcgct gaacagtttg agcctcggca gtgacaccgc gacggcgctg
    15181 ggcagtcgcg tggcgcgcac acagttaatt ggtctgctgg cgattaccgt gctttgtggt
    15241 agtgcgacgg cggtagttgg cccgattgcc tttatcggcc tgatgatgcc gcacatggcg
    15301 cgctggctgg taggtgccga tcatcgctgg tcgctgcccg tcacgctact tgctacccct
    15361 gccctgctgc tgtttgccga tatcatcggg cgggtgattg tgcccggcga gttgcgcgtt
    15421 tctgtggtca gtgcgtttat tggtgcaccg gtgctgatct tcctcgtgcg acgtaaaacg
    15481 cgaggtggtg catgatttac gtctctcgcc gattactcat cacctgtttg ctgcttattt
    15541 ccgcctgtgt ggttgcaggt atctggggat tacgcagcgg tgccgtcacg ctggaaacct
    15601 cgcaggtatt cgccgcgctg atgggcgatg cgccgcgcag tatgacgatg gtggtcaccg
    15661 aatggcgttt accacgcgtg ctgatggcgc tgttgattgg cgcggcactg ggcgtcagcg
    15721 gtgcgatttt tcagtcgctg atgcgtaacc cgctcggcag ccctgacgta atgggcttta
    15781 acaccggggc gtggagcggc gtgctggtgg cgatggtgct gtttggtcag gacctgacgg
    15841 ctatcgcgct ggcagcaatg gtgggcggca ttgtcacttc tctgttggtc tggctgctcg
    15901 cctggcgtaa cggtatcgac acctttcggt tgattattat cggcatcggc gttcgcgcca
    15961 tgctggtggc ctttaatacc tggctgttgc tgaaagcgtc tttagaaacg gcgctaacag
    16021 cgggtttgtg gaatgccgga tcgctcaatg gcctgacgtg ggcaaaaact tcgccttccg
    16081 cacccatcat tatattgatg ctcattgccg ccgccttgct ggtgcggcga atgcgcttgc
    16141 tggagatggg cgatgatacc gcctgcgcgc tgggcgtcag cgtcgagcgt tcgcgtctgt
    16201 taatgatgct ggttgcagtg gtgcttaccg ctgcggcaac ggcgcttgcc gggccgattt
    16261 cctttattgc tttagtcgcg ccgcacattg cccgacgcat tagcggcacc gctcgctggg
    16321 ggctgaccca ggcggcgctg tgcggagcgc tgttactgct ggcggccgat ctctgcgccc
    16381 aacaactgtt tatgccgtat caacttccgg ttggcgtcgt taccgtcagc ctcggcggta
    16441 tttaccttat cgtcttgtta attcaggagt ctcgcaaaaa atgaccgaat cagtagcccg
    16501 tttgcgcggc gaacagttaa ccctgggata tggcaaatat accgttgcgg aaaatctgac
    16561 tgtagaaatt cctgatggtc acttcacggc aattatcgga ccaaatggct gtggtaaatc
    16621 cacgttactg cgtaccttaa gccgcctgat gacgcctgct cacgggcatg tctggctgga
    16681 tggcgagcac attcaacatt acgccagtaa agaagttgca cgccggattg gtctgttggc
    16741 gcaaaatgct accacgccgg gcgatatcac cgtgcaggag ctggtggcgc gtggacgtta
    16801 tccgcatcaa ccgctgttta cccgctggcg caaagaggat gaagaagcgg taacgaaagc
    16861 gatgcaggcc acgggaataa ctcatctggc agatcaaagc gtggataccc tttctggcgg
    16921 acaacgccag cgagcgtgga tcgcgatggt gctggcccag gaaacggcaa ttatgctgct
    16981 cgacgaaccc acgacctggc tggatatcag tcatcagatt gatctactgg aactgttaag
    17041 cgaactgaac cgcgagaaag gctataccct ggcggcggtg ctgcacgatc ttaatcaggc
    17101 ctgtcgttac gccagccatt tgattgcatt gcgggaaggg aaaattgttg ctcagggagc
    17161 gccgaaggag attgtcactg ctgaactgat tgagcgcatt tatggtctgc gatgcatgat
    17221 cattgacgat ccggtagccg gaacgccgct tgtggtgccg ctcggacgaa cggcaccttc
    17281 aaccgcaaat agttaaacta agtggtctgc catcatggca tcctgttttc tggatgccat
    17341 cgcatagcgc aataacacgc taccacaagc caccatcccg ccgatcagcg cggaaaggat
    17401 cacaataatc gccttacccg gaccgtcttt tttcactggc aatgacgggc ttaactgata
    17461 tttaaacggc gtaaaattca catcgttgac atttgctttt gttaattgct cgacaagata
    17521 ctgccgatta cgtaattcac cgttcagttc cgcaacgtca gtaaccgctt tttcaatttc
    17581 cagtttgcgt tcaataccgt ctgcgccgag agaaatagag aaatcggggt catctttaac
    17641 ggcctgacca ttactgtata cgggcttttt aattcccgcc gcgttggcaa tgtcgagtga
    17701 ataattgagg cgctgaatgt ttgcatcaag ttgatttttc gttttaatgc gatcctgagc
    17761 cagtttttct ttttcaaact gggttttgat ttccagttta ttacggacgt tttcgagtga
    17821 ctctttcacc accaacgcag agatataatc gatatacccg ctcaaaacgg tctgcgcctc
    17881 ttcactggtt ggcgcggtaa aacttagcgt ccaggaggta tacagtgacg gttcatcttt
    17941 tttcttactg gcattgtcat caaccgcttt cattttttcg ctcaatgcaa caatagcgcg
    18001 atgcaaatcc agttcatcga ttttcgcctc ttttaattgg tccatcacat aaggtgatga
    18061 acgcaggtac tcttccagca agctaaccga ctgaaacttc ttgataaaca ggttaaatgc
    18121 ttctgtacga tcaattttga tatccagatc cagcactcga agcttagtga atgttttctc
    18181 cagctcttgc cactgaacag gttctggagg cgtgacaacc gccgcgctgg tccatttttg
    18241 cggcaggatg aaagagatca gcaagcctgc gcaggcaaac gcaaaaatga ccgccatgac
    18301 cgtttttttg gcccgccata aaactgcgat tagattaagt aaatcaattt cattattact
    18361 gggcgacgcc agaggatact caggaaaatg agcgtcactt ccctgtttaa tattcagtga
    18421 tgacatgggg tataacctga aaatgagtcc aataggggaa atctccacgg cgctcacttt
    18481 accagttggt gataattatc cgaagctgaa gtttgtaaat tccttccact gaatgcaaca
    18541 ttcactcta
//
LOCUS       DAMZII010000095        18333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZII010000095 DAMZII010000000
VERSION     DAMZII010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.095"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            7..81
                     /gene="tnaC"
                     /locus_tag="POA77_003236"
     CDS             7..81
                     /gene="tnaC"
                     /locus_tag="POA77_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDI6366620.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            302..1717
                     /gene="tnaA"
                     /locus_tag="POA77_003237"
     CDS             302..1717
                     /gene="tnaA"
                     /locus_tag="POA77_003237"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDI6366621.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1808..3055
                     /gene="tnaB"
                     /locus_tag="POA77_003238"
     CDS             1808..3055
                     /gene="tnaB"
                     /locus_tag="POA77_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDI6366622.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWIGNVFNVLPKFG"
     gene            3188..4363
                     /gene="mdtL"
                     /locus_tag="POA77_003239"
     CDS             3188..4363
                     /gene="mdtL"
                     /locus_tag="POA77_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDI6366623.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4338..5297
                     /gene="yidZ"
                     /locus_tag="POA77_003240"
     CDS             4338..5297
                     /gene="yidZ"
                     /locus_tag="POA77_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDI6366624.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5454..6203
                     /locus_tag="POA77_003241"
     CDS             5454..6203
                     /locus_tag="POA77_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI6366625.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6225..6791
                     /gene="yieF"
                     /locus_tag="POA77_003242"
                     /gene_synonym="chrR"
     CDS             6225..6791
                     /gene="yieF"
                     /locus_tag="POA77_003242"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDI6366626.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGVRCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6845..8182)
                     /gene="adeP"
                     /locus_tag="POA77_003243"
     CDS             complement(6845..8182)
                     /gene="adeP"
                     /locus_tag="POA77_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDI6366627.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGSPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8349..9014
                     /gene="yieH"
                     /locus_tag="POA77_003244"
     CDS             8349..9014
                     /gene="yieH"
                     /locus_tag="POA77_003244"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDI6366628.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9081..9554
                     /locus_tag="POA77_003245"
     CDS             9081..9554
                     /locus_tag="POA77_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366629.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVTCLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9603..10190
                     /locus_tag="POA77_003246"
     CDS             9603..10190
                     /locus_tag="POA77_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDI6366630.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10252..10974)
                     /locus_tag="POA77_003247"
     CDS             complement(10252..10974)
                     /locus_tag="POA77_003247"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6366631.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10989..12158)
                     /locus_tag="POA77_003248"
     CDS             complement(10989..12158)
                     /locus_tag="POA77_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6366632.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDECVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12185..13801)
                     /gene="bglH"
                     /locus_tag="POA77_003249"
     CDS             complement(12185..13801)
                     /gene="bglH"
                     /locus_tag="POA77_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDI6366633.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13887..15281)
                     /gene="bglB"
                     /locus_tag="POA77_003250"
     CDS             complement(13887..15281)
                     /gene="bglB"
                     /locus_tag="POA77_003250"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDI6366634.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15300..17177)
                     /gene="bglF"
                     /locus_tag="POA77_003251"
     CDS             complement(15300..17177)
                     /gene="bglF"
                     /locus_tag="POA77_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDI6366635.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17311..18147)
                     /gene="bglG"
                     /locus_tag="POA77_003252"
     CDS             complement(17311..18147)
                     /gene="bglG"
                     /locus_tag="POA77_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDI6366636.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4649 a         4556 c         4314 g         4814 t
ORIGIN      
        1 tgcattatga atatcttaca tatatgtgtg acctcaaaat ggttcaatat cgacaataag
       61 attgtcgatc accgccctta atttaccctt ctgtagccat caccagagcc aaaccgatta
      121 gattcaatgt gaacgattag tttgctatat cttaattttg ccttttgcaa aggccatctc
      181 tcgtttattt acttatttta gtaaatgatg gtgcttgtat atatacatgg cgaattaatg
      241 gatattgcag atgtaatatt cacagggatc actgtaatta aaataaatga aggattatgt
      301 aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg agccagtaaa
      361 acgtactact cgcgcttatc gtgaagaagc aattattaaa tccggtatga acccgttcct
      421 gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg gggcggtaac
      481 ccaaagtatg caggcagcga tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta
      541 ctatgcgtta gccgagtcag tgaaaaatat ctttggttat caatacacca ttccgactca
      601 ccagggccgt ggcgcagagc aaatttatat tccggtactg attaaaaaac gcgagcagga
      661 aaaaggcctg gatcgcagca aaatggtggc attctctaac tatttctttg ataccacgca
      721 gggccatagc cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga
      781 tactggcgtg cgttacgact ttaaaggtaa ctttgacctt gagggattag aacgcggtat
      841 tgaagaagtt ggcccgaata acgtgccgta tatcgttgca accatcacca gtaactctgc
      901 aggtggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg cgaagaaata
      961 cgatattccg gtggtaatgg actccgcacg ctttgctgaa aacgcctatt tcatcaagca
     1021 gcgtgaagca gaatacaaag actggaccat cgagcagatc acccgcgaaa cctacaaata
     1081 tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtaccgatgg gcggcttgct
     1141 gtgcatgaaa gacgacagct tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt
     1201 gcaggaaggg ttcccgacat atggcggcct ggaaggcggc gcgatggagc gtctggcggt
     1261 aggtctgtat gacggcatga atctcgactg gctggcttat cgtatcgcgc aggtacagta
     1321 tctggtcgat ggtctggaag agattggcgt tgtctgccag caggcgggcg gtcacgcggc
     1381 attcgttgat gccggtaaac tgctgccgca tatcccggca gaccagttcc cggcacaggc
     1441 gctggcgtgc gagctgtata aagtcgccgg tatccgtgcg gtagaaattg gctctttcct
     1501 gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggctgaac tgctgcgttt
     1561 aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg aagcctttaa
     1621 acatgtgaaa gagaacgcgg cgaatattaa aggattaacc tttacctacg aaccaaaagt
     1681 attgcgtcac ttcaccgcaa aactgaaaga agtttaatta atactacaga gtggctataa
     1741 ggatgttagc cactctctta ccctacatcc tcaataacaa aaatagcctt cctctaaagg
     1801 tggcatcatg actgatcaag ctgaaaaaaa gcactctgca ttttggggtg ttatggttat
     1861 agcaggtaca gtaattggtg gaggtatgtt tgctttacct gttgatcttg ccggtgcctg
     1921 gtttttctgg ggtgccttta tccttatcat tgcctggttt tcaatgcttc attccgggtt
     1981 attgttatta gaagcaaatt taaattatcc tgtcggctcc agttttaaca ccatcaccaa
     2041 agatttaatc ggtaacacct ggaacattat cagcggtatt accgttgcct tcgttctcta
     2101 tatcctcact tatgcctata tctctgctaa tggtgcgatc attagtgaaa cgatatcaat
     2161 gaatttgggc tatcacgcta atccacgtat tgtcgggatc tgcacagcca ttttcgttgc
     2221 cagcgtattg tggataagtt cgttagccgc cagtcgcatt acctcattgt tcctcgggct
     2281 gaagattatc tcctttgtga tcgtgtttgg ttctttcttc ttccaggtcg attactccat
     2341 cctgcgcgat gccaccagca ccactgcggg aacgtcttac ttcccgtata tctttatggc
     2401 tttgccggtg tgtctggcgt catttggttt ccacggcaat attcccagcc tgattatttg
     2461 ctatggaaaa cgcaaagata agttaatcaa aagcgtggta tttggttcgc tgctggcgct
     2521 ggtgatttat ctcttctggc tctattgcac gatggggaat attccgcgcg aaagctttaa
     2581 ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa
     2641 acagcacggc attatcgagt tttgcctgct ggtgttctct aacttagctg ttgccagttc
     2701 gttctttggt gtcacgctgg ggttgttcga ttatctggcg gacctgttta agattgataa
     2761 ctcccacggc gggcgtttca aaaccgtgct gttaaccttc ctgccacctg cgttgttgta
     2821 tctgatcttc ccgaacggct ttatttacgg gatcggcggt gccgggctgt gcgccaccat
     2881 ctgggcggtc attattcccg cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca
     2941 gatgttcacg gtctggggcg gcaatcttat tccggcgatt gtcattctct ttggcataac
     3001 cgtaatttta tgctggatcg gtaacgtctt taacgtgtta cctaaatttg gctgatccct
     3061 tcaagaagcc agccatttcg ctggcttctt tcctgtcagg aaatctcttt tgtccaaatg
     3121 gcaactcgcc tgattctcct tcaccacgta tgctttgcgt caccttacta tcaggacgct
     3181 ttagcccatg tcccgctttt taatttgtag ttttgccctg gttttacttt atcccgccgg
     3241 gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc
     3301 gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga tgttatttgc
     3361 cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg cggcgctatt
     3421 tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc ttgcaggccg
     3481 atttctacag gggttgggcg caggctgttg ttacgtagtg gcgttcgcca ttttgcgcga
     3541 cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta ttacctgcat
     3601 cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc cgtggcagag
     3661 tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt tgtttatttt
     3721 aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc
     3781 gctgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg tttcggtgat
     3841 cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatgggtt ttgagcgcgg
     3901 agaatacgcc accattatgg cgttgactgc tggcgtcagc atgaccgttt cattctccac
     3961 gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc aggtgttatt
     4021 ccttgcagcg gggatcaccc ttgccgtttc accttcccat gcggtttctc tgtttggtat
     4081 cacgctgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc aggcgttagg
     4141 accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg
     4201 ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga atatgctgat
     4261 cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg tcgcgcctgg
     4321 acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat ctgctgctct
     4381 gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga
     4441 ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac gacccgctct
     4501 ttgtgaactc accgctgggc ctgtcgccca caccgctaat ggtcagcatg gagcaaaatc
     4561 tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac cagacaccgc
     4621 gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg cttaatgcgc
     4681 tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt aactgggatt
     4741 acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa
     4801 gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat tatgaagtgc
     4861 tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg catgaaacgt
     4921 ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa cagagcgaca
     4981 cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt gctatgagcc
     5041 tgccggaatt cgagcagtca ctgtttatgg cagcgcaacc cgacaatctg ctactggcga
     5101 ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg gttgctcttc
     5161 ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc ctgctgtggc
     5221 ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt aaaaaccttt
     5281 acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa taaaatgtaa
     5341 aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg tccatcattc
     5401 cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga aaaatggcga
     5461 ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt ctcccctccc
     5521 agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt ctggcgtcca
     5581 gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg ccggaaatcg
     5641 tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg ttttctattt
     5701 cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt ggcctcgata
     5761 tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat aaccacacct
     5821 tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa gcgcgggcgc
     5881 agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg aatgacgatc
     5941 cgcgcgatct gcagctgctg cccattgccg gacgcctgaa atgtgctcat gtaaatcatg
     6001 tagaagcgtt atgcgacgcg gaggacgtgc tagtgtggtc cgtggcggtc acgcccgcga
     6061 ttgaaaagct cagtgtctgg gagttagatg gcaaacacag ctggaaaagc ctgccagata
     6121 ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag ctctctaccg
     6181 tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct gaaaaattgc
     6241 aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg gttgcacgta
     6301 ccctgccgaa aattgctccg gcaagcatgg aagtcaatgc gttaccttcc attgccgaca
     6361 ttcctctgta tgacgctgac gtacagcagg aagacggttt tccagcaacg gttgaagctc
     6421 tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa tataactact
     6481 cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg gatcaaccgc
     6541 tggcaggtaa accggtgttg attcagacca gctcaatggg cgtgattggc ggcgtgcgct
     6601 gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg aacaagccgg
     6661 aatttatggg cggcgtgatt cagaacaaag ttgatccgca aaccggagaa gtgattgatc
     6721 agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt attcagcgag
     6781 ttaagatcta aataaaaaac ccgccagtaa tcatgcatgg cgggttttta atgcgctatc
     6841 gattttaatg agcgtcgata aatacaatct tcaggataaa catcagcgca acgatgatta
     6901 cgcacgggct aaggtcacgc agacgcccgg taccaatctt catcacgcag taggagataa
     6961 agcccagcgc aataccttcg gtaatcgaaa agctgaacgg catcatcacg gcggtaataa
     7021 acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt gaggtcatca
     7081 acacgccaac gtagatcaac gcgccagctg cagcgtagcc tggcaccatt cccgccagcg
     7141 gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc agaccggtac
     7201 gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa gaagtaccga
     7261 taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcctgcttc atgcgcggga
     7321 atttaccctt ctcatccgcc aggcctgctt tatcggtcac gccaatcagc gtaccggagg
     7381 agtcaaacag gttgaccagc atgaaagaga aaatcacccc tgccagcccg aggttaaacg
     7441 acccggctaa atctacatgg cccacgacag tcattacgct cggcggcgca gaaacgatgc
     7501 cattgtagtg gacatcaccc agcatccagc ccagcagcgt tgtcaccacg atggaaacca
     7561 gcaccgccgc gtgaatgttg cgcgaggcca gaatagcgat gatgaagaag ccgaggatcc
     7621 ccagcagcac gctgtgagaa gtcaggttac cgatgctgac cagcgtttcc gggttagcga
     7681 caatcacacc tgcattcttc agtcccatca taccaatgaa cagaccgata ccgctggtga
     7741 tgcccacacg cagacttacc ggaatgttag caatcatcca gtaacgaacg cggaaaatcg
     7801 tcagtaacag cagaccaatc gcgccccaga agattgcgcc catcccgact tgccacggcg
     7861 agcccatagc ctgtacaacg acaaaagcga agaatgcatt caggcccata gcgggtgcca
     7921 gtgcaactgg caggttagca aacagtccca tcataatact gccgaatgca gcaatcagac
     7981 aggtagtaac gaagacggcg ctggtatcca tgccagcaac gccaagaatt tgcgggttaa
     8041 caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact tcggtccgtg
     8101 ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg ccagaagcct
     8161 gggttgtgtg ttgttgactc attatctatt tccgaacaag gagggaaaat ccgtcgctat
     8221 cgtataccaa aatgagacaa tatggacggc tgaagataac tttttttcat tgtatttgct
     8281 tatacggcaa cgattgcgtc atgcgaattg gcatgacgaa aacgttaaac taacgataaa
     8341 ggaaagacat gtcccagata gaagcggtat ttttcgactg cgacggtacg ctggtcgaca
     8401 gtgaagtcat ttgctcccgc gcatatgtaa cgatgtttca ggaatttggt attacgctcg
     8461 atcctgaaga ggtattcaaa cgtttcaaag gtgtaaaact gtacgaaatt atcgatattg
     8521 tttcccttga acatggtgtt acgttagcga aaacagaagc tgaacacgtt taccgtgcag
     8581 aagtcgctcg gctgttcgat tcagaactgg aagccatcga aggggctgga gcgctcctgt
     8641 cagcgatcac tgcgccaatg tgtgtggtat ctaacggccc aaataacaaa atgcagcatt
     8701 ctatgggcaa gctgaatatg ttgcactact tcccggataa actgttcagc ggctacgata
     8761 ttcagcgctg gaagccagac ccggcgttaa tgttccatgc ggcaaaagcg atgaatgtaa
     8821 atgtagaaaa ctgcattctg gttgatgact cagttgccgg tgcgcaatct ggtatcgacg
     8881 caggtatgga agtgttctac ttctgcgccg acccgcacaa taagccgatc gttcacccga
     8941 aagtcaccac ctttacccat ctttcgcagt taccggaact gtggaaagcg cgtggttggg
     9001 atattacggc atagttcttc acactccctt cacttacccc gcttaaattg gcgctcaaag
     9061 gtaagtaaag ggtgtttgat atgtctgttt cacgtcgggt aatacatcac ggactttatt
     9121 ttgcggtttt aggaccgtta attggtgttc tgtttcttgt cctctacata ttcttcgcaa
     9181 aagaaccgct ggttcttttg gtaataatac aggtacttcc attgtttctc ttattgtcga
     9241 ttactacggg agctattcct gcattgttaa ccggcgtgat ggtcacctgt ctgccggaaa
     9301 agatcgggtc acagaaaaat tatcgttgtc tggctggtgg cataggtggc gtcgttatca
     9361 ccgagatcta ttgtgcagtt attgtacata ttaagggcat ggcttcctcg gagttgtttg
     9421 aaaacattct ttctggtgac agtctcgttg tccgcatcat tcctgcattg ctggcaggtg
     9481 tggtgatgag cagaatcatt acccgtctgc ccggattgga tatttcatgt cctgaaacag
     9541 actctttaag ttaagcggga tactttatct ttgggctact caaaagcaga caggatgtta
     9601 ctatgactca aaatatcagg ccgttacccc aattcaaata tcatcccaag ccactggaaa
     9661 caggcgcatt tgaacaggat aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag
     9721 tttattactc gggtcccttt tattgcgttg atgaagttga acatctctgt ccgtggtgta
     9781 ttgcggacgg ttctgctgct gaaaagtttg caggtagttt tcaggatgat gccagcatag
     9841 aaggtgttga atttgagtat gatgaagagg acgaatttgc cggtattaag aacacatatc
     9901 ctgatgaaat gctgaaagag ttggttgaac gcacgccagg ttatcatgga tggcaacagg
     9961 aattctggct tgcgcattgt ggcgatttct gtgcttttat cggctatgtg ggctggaatg
    10021 atataaaaga tcgcctcgat gaatttgcca accttgaaga agattgtgag aatttcggta
    10081 ttagaaattc tgatctagct aaatgcctgc aaaagcgtgg tgattgtcag ggttatctct
    10141 tccgctgtct ccactgcggc aagctgagac tgtggggtga tttttcgtag ttatttaaat
    10201 aatgagaaca ggccggagcg taattcatac atccggcctt atttcttcag cttaaccgag
    10261 cgctaattct gcggcagcgg ctttatcagc gattaccatc agcgaaggat gcagctgcaa
    10321 cacggaagct ggaacatctt cggtaaccgg accttgcagc acatttttca gtgcctgcgc
    10381 tttacctgcg ccactgacaa ttatcagcaa gtttttcgcc gccatgatgc ttttcggccc
    10441 catcgtcacg tagctgtcag gcactaacga aaagtcgccg cccaactcac catgcgccac
    10501 aatatcaacc atctctccct ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt
    10561 attcggcaaa ttgccacaaa aatgaccatc tgcacctaat cccagcacca ccaaatccag
    10621 gccgccttca cgcgccagtt tctgatcatg ctcgcggtag ttatcaatag tgagcttctg
    10681 gatattctct tctttgatcc ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt
    10741 tacgccttct ccctctttgc cacgaaaggg aatttcatcg aaattataga aatagcagtt
    10801 atcgtaccag ggcttacctt taaccagggt ggtgaggtat tcatacatgc ctttgggcgt
    10861 gctaccggcg gtaattgcca ggttaacacg acgcgtcttc gacatataac caagcagatg
    10921 gtgtgccgcg acacggctca tttcctggta atcttcggta atgattaatt tcatcttaat
    10981 gccttatttt atttaaataa tttctgtacg aaggctgcat aggcaggccg ccagacatcc
    11041 atttcatggt tcaggccggg atattcctgg taatcaaact taattttttt ctgctcaagc
    11101 tcagttttca gcccggcgat atccttgccg gttacgacat ctttatcacc aacgaccaca
    11161 gtaaaattac gtagttgctg gttaatagct gccggatcgt tcagccgggc cgcgacacat
    11221 tcatccggta cggttgtcgt ggtaacacca ctgaatgtgg ccagccagcc aaagctttcc
    11281 agatgattca ttccggaaac cagcgcctgg tacccgcctt gtgaaagccc tgccagcgcg
    11341 cgaccatcgg catctttacg gacattaaaa cgcttgctaa tcagcgggat aatatcgttc
    11401 atcagttcgc gatctgccgc tttagcattc agcggataaa agactttacg tctttcctga
    11461 ggaacgaaat cttcgggaat aatgcccttc gcatcggttt cggtatccgg gatcaccacc
    11521 agcatcggtt taattttccc ttcagccagc aggttatcca tgatttgcgg gatacgcccc
    11581 tgatcgatag cggaacgtcc ggtatcacca aaaccgtgat agaaatagag cactggcaaa
    11641 ggctcgccca tgccggtgta tcctggcggg gtccagacat acatctgacg ttcagattgc
    11701 aatgcgttgg agtggtaagt tatggcgatc aaatcaccat gcgcaacaga acgcgtatcc
    11761 agataactgc ctggcaccag aatcatactg gagttaacct ggcgctgagg cttagtcatt
    11821 gcggtgcctg tatcaatgct gcgtacacca tcaacattga aaaaatactc gtacaggttg
    11881 cctttcagga taggtgtgcg ccacgaccag actcctgctt cgtctttggt catcgggtga
    11941 atattgtccg gaaccggaac acccaccact acagagacat tttttgcccc aggggcaaag
    12001 tagcgaaagg tcacgctgtt atcggcattg acctgagtca catactgttt caccgggata
    12061 gtgggtgccg ggctggctgg catatccgct gctatggccc actgagcaga aataccgctg
    12121 gcaatagcca gcgttaaagc agcaattttt atattcatcg taatattcct ttcattaagt
    12181 tccattacca ccagatttca gcctgggcac cgacagcaaa ctggtcgtct ttattgtctt
    12241 cgaaggtgaa gccatccagt tcgttttcca gggctttgat atacgtggcg tagaaacgaa
    12301 tttccggacg cgaggtcaac atactggtat tgactttaaa ggtatggaag agcgtggttt
    12361 tataaccaga ctcattaaat ttattactat tcgcatcttt gttttgctgg gtgaaatagc
    12421 ccagttcaac acctgtctgg ttatattggt cccaaatata ggccgggcga acaaccgcac
    12481 gaatagattc gaaatcagag tgtgcgcctg tttcgtagct ataaatatcg ttaccgaagg
    12541 agtaaacaat cgcgttagcc acaataaaat gatcgccgat ataggcttcg ccctgcgagg
    12601 tcagacgaac tgctgttccg ccggtgtgat caccataata acgaccatta aaggtggtaa
    12661 atggacttgc gccagcataa cggccaaagt tactggcgat agagttattc gcaaccagga
    12721 aggagaattc gttgaagcca cctttatcaa atttctgcgt taaagatgtg ccgaacatcc
    12781 aggtatcttt ccagtcataa tacccgttat tatcctgatt atctttttcc gatgcgcttt
    12841 cgtttgccgt gacataacga ccactcacca ttaaggtcgc tttatcccat aacgggatat
    12901 ctttatagcg taaatcaata gtattggtat taatctgctg tttgttttgc aggctgcgat
    12961 cgtaatcatc gacatcttcg cgaaccagcg cgatatcaat tttacccgga ccgactttcc
    13021 agttttccag acctacaccc gctgcggcat cagtacgctg cgttttccag tccagcatct
    13081 ggatttcaat tttcggcgca ccgtgtttac ccacccagaa atcagcctct ggcgcaaagg
    13141 gcaggaaacc tttggtggta acgtacatat cggagaactg cataaagttc tcgccaccgg
    13201 cgttatcgcc aaaccagccg gtagagtact gctgaccaac gttaccatcc atcatcacaa
    13261 cggcatcaac ccgtttgccg ttttcgttgt agacacgttg ttttaactgc aaatcaaacc
    13321 agccggagta ttcgttacca aagcggccca gagaaccaat cgcccatgat ttaggtgaac
    13381 catgagaggc ggtcccccaa ccagaacggt aatagccgtt gtaactaaag ccaatttcat
    13441 ctttcacaaa cttactgaaa tctttcagag tcatggaaga ataaatcgag cctgtctcac
    13501 tggcattttt ctcttcattt ttgaccagta cagcatcagg ttttgcggca ctactggtcg
    13561 ggaacggatt ggcggcatac ccttgatcat tcgtacttac gctacgattc accgtcgctg
    13621 gcgtatattt tttcttctct tcatctttat actttttgag ttcgctttgc gtttctctta
    13681 acgctttttc taataactca aggcgatgtt ccaccgttaa tgattcagcc aatgattgtg
    13741 cagaaaaggc taacggtgcc attaataaga tggcagaggt aataagattt cgtctaaaca
    13801 taatatccct ttatggtgca aagaaagaat taacgtatcg catccagact gttctgaatg
    13861 cgacgataat taaggtgctt tattggttat ttttttaatg acagcccccg cgttttgatc
    13921 acttctgcat accatccgaa gcttttctta cgcgttcttg tgaggcttcc ttcgccatta
    13981 tcatcacgat ccacataaat aaagccgtag cgcttagaca tttgtgaatg agatgcactg
    14041 actaaatcaa ttggccccca actggtgtac cccataatat ccacaccatc ggcaatcgct
    14101 tcatttacct gtaccaggtg atcgtttaaa taggcaattc gataatcgtc ctgtatcgaa
    14161 ccatccgctt caacgctgtc ttttgcgcct aatccgttct cgacaataaa taacggtttt
    14221 tgataacgat cccaaagcgt atttaacaga acccgtaatc caaccgaatc aatttgccac
    14281 ccccactctg aacttttcag atgcggattg gggatcatat tcagtatgtt gccctgcgca
    14341 tttttattaa tgctttcgtc gtgggaaaca caaccagtca tgtaataact aaaagagatg
    14401 aaatcgacgg tatgttttaa atcttctgcg tcactttcag tcatctcaat ggtgatattg
    14461 tggtcgcgga agaaacgctg catatagccg ggatactggc cacgcgcctg aacatcacca
    14521 aagaacatcc agcgccggtt ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg
    14581 agggggtaaa ccagcccacc gagaagcata ttgccgattt tcgcttcggg gagcaggcta
    14641 tgacaggctt taactgcccg cgcactggca accagttgat ggtggatagc ctgataaact
    14701 tccgcctcgc cactctcttc tgccagcccc acgcccgtga atggctcgtg taacgacatg
    14761 ttgatttcat taaacgtcag ccataacgcc actttatgtt ggtagcgagt aaagaccgtg
    14821 cgggcgtaat gctcgaagtg atcgatgacc gctcgattag cccaaccgcc gtagtttttc
    14881 accagcccat atggcatttc gtaatgggat aacgttacca gcggcttgat ccccgcctgc
    14941 gccatttcat caaacagccg atcgtaaaac gctaaccccg cttcattcgg ttcgacttcg
    15001 tcgccctgag ggaaaattcg cgcccaggca atggaaatac gcagacaggt gaagcccatc
    15061 tcggcaaata acgcgatatc ttccgggtaa cggtgataaa aatcgatggc gacatctttg
    15121 atattctctt tccccaggat gcgcggttcc atttttccca ttacgccatg aggctgtaaa
    15181 tctgaggtcg agatcccttt gccatcttcc tgccaggcac cttccacctg attggcagct
    15241 gttgcgccac cccaaagaaa tgtttctgga aatgctttca taattaactc cttttatcgt
    15301 tagcgaatga tggataacag cggttcacct gcgcttatct gcgccgtgcc gtggggtaat
    15361 acgtccgtaa aatcatcgct attactgatt aataccggcg tcgtcagatc aaatccggcc
    15421 tcgcgaatag cagggatatc aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc
    15481 acgttgacgt gagcggaaaa gaatttgccg tccagtttta cggtgtcgat accgacatga
    15541 atcaggagct ccacaccatc atctgactca atgccaatgg cgtgtaatgt ggcgaacaac
    15601 gaagcaattc gacccgcaac cggagaacgc acttcaccaa ccgagggcag aatggcgata
    15661 cctttaccca acaggccact ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc
    15721 tctcccgtca tcggtgaaca gataccgccc tgctcaggtg gtgtaataac ctctggtgtt
    15781 ttctcttgcg gggcaccctg cgctggctga cgtttagcgg tgatgaaatg aagcatcacc
    15841 gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc ccagacggtg
    15901 aaatcaattc ccgttgacgg gatggtttgc atgaaggtga aaatacttgg caaaccaaag
    15961 gagtagactt tcgtttgcgc gtagccaatg atggtggccc ccaaagcccc actgatacag
    16021 gcgataacaa aggggtactt acgcggcaga ttaacgccat ataccgctgg ttcggtgata
    16081 ccaaacaggc ccgtcaacgc cgctgatccc gccaccactt ttttctgcgc atcgcgttcg
    16141 cagaggaaga cgccgagcgc cgccccgacc tgcgccataa tggcgggcat taacagcggg
    16201 atcatggtgt cgtagcccag cacggtgaag ttattgatac atagcggcac caggccccag
    16261 tgcagtccga acatgacgaa gatttgccag aagccgccca ttaccgcgcc tgcaaatgca
    16321 ggaaccgcct gataaagcca gagataaccg gcggcaatca gttcgcttat ccaggttgat
    16381 agcggcccca ccagcagaaa ggtgacgggt gtgataacca tcagacatag caatggtgtg
    16441 aagaaatttt tgattgccga cggtaaccac gcattaagtc ggcgttccag aatgctgcac
    16501 aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa tgtgaccgga
    16561 atacccagga aatccagccc cagctcatcc gctttttgcc cgttctcgaa agcagtcaga
    16621 attaatggat gcactaacgc tccaccaatc accatggcag taaatggatt accgccgaag
    16681 cgtttccccg cggtgtatcc caggattatc gggaagaacc aaaacaaggc atcactggcg
    16741 ctgaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt gagcgccaga
    16801 gccagcatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg cgtaaaaata
    16861 cctgaaataa cataaacaaa gcggtttagc agattacctt tctcatcatt ttccggtgcc
    16921 tgttgcgctt tttcgccaag gcctgccacg ctgttgaccg ccaggaagac atcggccaca
    16981 tggttaccta tgaccacctg aaactgacca ccgctttcca ccaccataat aataccgggg
    17041 gtctttttca gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc
    17101 gttgcgcaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc gactattttt
    17161 ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaac
    17221 ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc cgcttagtgc
    17281 ggtaacaatc ctttactcag taataatatt tcagtgttct ttgcgcacgc gctctatatt
    17341 tatggctaaa aacataatct ctgcgggtga aattttacgt tgatactgca aaccaataaa
    17401 aatggcgatc cgttccgcac attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg
    17461 taatgattca tcactatcgt taattgaagc atgttcaaga atacgccagg ataaaaactt
    17521 cagatgtgta accagtcgct gataactcaa gctttcttcc tggtaattaa ggctgaactg
    17581 aaattttatt aattgcagca tttcgcgcat taactgcgtg acacctgcaa catcctccat
    17641 atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt catctttcgg
    17701 taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta gctggaactc
    17761 tttcgggtaa agccgctgga tatcccacag caacgggttg ggcagcaaca cgttttgctg
    17821 aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgtaa
    17881 ttttcccaag cgctcctgcg ctaaagagat aatacgatca caggttgcca tcacctcaag
    17941 aggaatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata
    18001 ctctttttct attccacttg agttaattct ttcgccagcg cgtttttgaa agccaattcc
    18061 tcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt
    18121 gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataacaa aaaaacccga
    18181 cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc
    18241 atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa ttgcgtgcag
    18301 agaaaatagc aatgcgctat tgataaaaat atg
//
LOCUS       DAMZII010000096        18204 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZII010000096 DAMZII010000000
VERSION     DAMZII010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.096"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(389..1981)
                     /gene="lsrK"
                     /locus_tag="POA77_003253"
     CDS             complement(389..1981)
                     /gene="lsrK"
                     /locus_tag="POA77_003253"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDI6366637.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(2060..3013)
                     /gene="lsrR"
                     /locus_tag="POA77_003254"
     CDS             complement(2060..3013)
                     /gene="lsrR"
                     /locus_tag="POA77_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDI6366638.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3262..4797
                     /gene="lsrA"
                     /locus_tag="POA77_003255"
     CDS             3262..4797
                     /gene="lsrA"
                     /locus_tag="POA77_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDI6366639.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4791..5819
                     /gene="lsrC"
                     /locus_tag="POA77_003256"
     CDS             4791..5819
                     /gene="lsrC"
                     /locus_tag="POA77_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDI6366640.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5819..6811
                     /gene="lsrD"
                     /locus_tag="POA77_003257"
     CDS             5819..6811
                     /gene="lsrD"
                     /locus_tag="POA77_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDI6366641.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAIAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6823..7845
                     /gene="lsrB"
                     /locus_tag="POA77_003258"
     CDS             6823..7845
                     /gene="lsrB"
                     /locus_tag="POA77_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDI6366642.1"
                     /translation="MTLHRFKKIALLSVLGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7872..8747
                     /gene="lsrF"
                     /locus_tag="POA77_003259"
     CDS             7872..8747
                     /gene="lsrF"
                     /locus_tag="POA77_003259"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDI6366643.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8771..9061
                     /gene="lsrG"
                     /locus_tag="POA77_003260"
     CDS             8771..9061
                     /gene="lsrG"
                     /locus_tag="POA77_003260"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDI6366644.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9118..9876
                     /gene="tam"
                     /locus_tag="POA77_003261"
     CDS             9118..9876
                     /gene="tam"
                     /locus_tag="POA77_003261"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDI6366645.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GIHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLAESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9880..10794)
                     /locus_tag="POA77_003262"
     CDS             complement(9880..10794)
                     /locus_tag="POA77_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDI6366646.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(11001..12452)
                     /gene="uxaB"
                     /locus_tag="POA77_003263"
     CDS             complement(11001..12452)
                     /gene="uxaB"
                     /locus_tag="POA77_003263"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDI6366647.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12679..14097)
                     /locus_tag="POA77_003264"
     CDS             complement(12679..14097)
                     /locus_tag="POA77_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6366648.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14236..14595)
                     /locus_tag="POA77_003265"
     CDS             complement(14236..14595)
                     /locus_tag="POA77_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDI6366649.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14595..15521)
                     /gene="glsB"
                     /locus_tag="POA77_003266"
     CDS             complement(14595..15521)
                     /gene="glsB"
                     /locus_tag="POA77_003266"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDI6366650.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15585..16973)
                     /gene="sad"
                     /locus_tag="POA77_003267"
     CDS             complement(15585..16973)
                     /gene="sad"
                     /locus_tag="POA77_003267"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDI6366651.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            17074..17955
                     /locus_tag="POA77_003268"
     CDS             17074..17955
                     /locus_tag="POA77_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6366652.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            18032..>18204
                     /gene="yneK"
                     /locus_tag="POA77_003269"
     CDS             18032..>18204
                     /gene="yneK"
                     /locus_tag="POA77_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDI6366653.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATK"
BASE COUNT         4590 a         4622 c         4523 g         4469 t
ORIGIN      
        1 ctttcgaatt gatagattaa aaaatgcata gttcttattt tatttaaaaa tgaactatga
       61 gttattgttg attaataaga aaactgtttc tttcaatagg aaaattatca tcatcatatt
      121 taacaaagaa tagcactaat tgctaaaaat cgaagtttat taaacccctt ttgcatccat
      181 tagtgtcttt aggaatattc gctataaaat atgggttgtg cttaatgctt taagaaaaat
      241 agcaattttc ccttgaatat agtactggtg atggaacgat gaatctgcaa gctggctttt
      301 aacaagccag ctctaaaaga agggaaataa gaataactat actcaaaaac taacagccac
      361 ggtcatcatg atgtggctgt caatgaaact ataacccagg cgctttccat aacgacgtcg
      421 tcagtccatg atcaaccagc cccagctgat cctgataaac tgcctgccac ttatcgcgtg
      481 aatcctgata aagttcatgc ttttccgggt ctggtgtgtg cgtccgttcc cagcgaacca
      541 ggcgttctcc ggtttctgcc attgatgaaa aaattccggc accgacgcca gctgcaatgg
      601 cacatcctaa tgcagtggct tctttgacca ccggaatatt gacgggtaat cccgagacat
      661 cagcgagaat ttgactccat aatttccctt ttgaacctcc gcctgcaaag actaacgatg
      721 aaggatgaat attcgagaaa tcagcaattt gctgcaagtt acacgctgat acaatcgccg
      781 cattttcttc cagcgcacgg aacaatgtcg ctttgttaca tttatccggg tcaatggaca
      841 agttaataaa ggaaggcgca gcgtgatacc aggttttaaa gcgcattctg tcggagaaga
      901 tcggcattac gccccacgac ccaggcggca cccgactggc catctcttcc agcagcgtat
      961 aggtgtcgat gcctaaacgt tcggcaatca gtttttcttc ggcacagaaa gcatcgcgga
     1021 accagcgcat ggtgagtccg gtaaaaaagc ttatagattc agcttgtacc atgccaggaa
     1081 taacatgagg attaacgcgc acgttcattt ctgggtctgt caccggcgcg gctaaattta
     1141 caacttgctg ccagaatgtg ccgccaagaa ccgcggtttg tgccggacgc acaacgccta
     1201 acccaaggca accaagctgc acgtcgcctc ctccaacgac caccggagtg cccgccttca
     1261 gaccgcagag ttccgccgct tgtgaactta ccacgcccag caatgtgccg gtttctttga
     1321 caggagaaag aatatcggca cgcaggccag ccatatccag caatgcaggt ttccagtcac
     1381 gggtggttag atcaagaagt cccgtggtgc cagcgttaga gggatccacc gccagttcgc
     1441 cgctgagcat ataggccagc cagtcgctga tcatggtgat ggttgatgcc tgacggtaaa
     1501 tatcggaacg atggtgcgcc agccaaagta atctggggat ggcacttaaa gccagtgttt
     1561 gtccggtcgc gcgataaact tcgttttcaa aggtattgtt gtgcagttct ttaagttcgc
     1621 taacttcgcg tgccgctctg gcatccacat tggcgcaggc ccagatcggg gctccttcat
     1681 tattatataa aacaatgcct tcacgcatcg aacatgccga aacggcagcg atatactccg
     1741 gggctatgcc ggcgttgtgc agcgcctggc gcatacactc acacgccagt tgccagtttt
     1801 tgttgagatc aaattccata gaaccaggaa cgtccggtac tgccaggtgc cgccactccg
     1861 cctgtcccac tgctatttga ttgccttcca ggtcgaatat cacagcccga atacttccgg
     1921 tacctgcatc cagcgccatc aggtaatact ttgattctga aggggtaaag agtcgagcca
     1981 ttagattatc ctcggcttat gcgttctgtc tgttcctcta tacgttctcc atcattcccg
     2041 gtaataaggt catgcaaatt taactacgta aaatcgccgc tgctgtgtcc tgatcggtaa
     2101 ccagtgcgtt gatataaccg cctttcattg cagcggcaat tgcttcggct ttattttctc
     2161 cccctgccac gccaacccgg acgggtatgg tcttcagcga gcttaaaggt aagccaatca
     2221 gttcgttatg tattttaata tccgtgacaa cgtcaccttt tgcatcaaaa aagtagccta
     2281 aaatgtcgcc aaccgcccct tttcgaccaa tcattaactg ttcgccctgg ctgatataac
     2341 cggagcgaat gatcgtcgcg tcgtcctgtt gactgacggc cccaatgccg acaatcgcca
     2401 catcggctgc ttgcgcggct aacagaacat ctttgacgca attttcattt tttagcgtac
     2461 gggcaatgtc agcggaggat gcccgcaacg gagccggaat aatattcaca ctgcacgccg
     2521 cgttaagctg cccgataccc gtcatataag aaccgacgcc accggagagc gtgaccaggc
     2581 gaatttgctg tgacgaaata aaaccactta agcgttgcag cgtattcatg gttgcctcgc
     2641 caaaaccaat cgccagcatc tgttgtggtt gaagtaaact catcaacata tgcgccgcgc
     2701 ctatccccag tcgcccgccg acatcagcat ccgcaagccc agggatcacc cggacatgtt
     2761 gcagcgaaaa ctgacgacgt aattgagttt catattccag acagccttca aagcgagaat
     2821 taatctgtac gcgaataatg ccggactgat gccctttctc cagcaatcgc gatactttca
     2881 aacgtgtcag gccgagacga tcgctgatct cactctgggt cagcccgtcg tggtagtaaa
     2941 accacgcgat ccgcgcgacc tgttcttctt cacacattcc ctgttctgaa attaccgaat
     3001 cgttgattgt cataattcat tcttcacttt gaacatattt aaatctttaa tgcaattgtt
     3061 cagttcttgc gcatttatat ctgtgatggc aaccacagtt tgactctacg agcatgaaca
     3121 aacgcaaccg tgaaaatcaa aatagcataa attgtgatct attcgtcgga attatgtgca
     3181 atgtccacct aaggttatga acaaattaaa agcagaaata catttgttca aaactcacct
     3241 gcaaaactga acgggggaaa tatgcaaacg agtgataccc gcgcgttacc gctactttgc
     3301 gcccgctcgg tttataaaca gtattcaggg gttaatgtcc tgaaaggcat cgattttacg
     3361 ttgcaccagg gggaggtcca cgccctgctc ggcggcaatg gtgccggtaa atcgacgtta
     3421 atgaagatta ttgccggtat tacccctgct gatagcggta cgctggagat tgggggcaac
     3481 aactacgtca gattaacgcc agttcatgct catcagctgg gtatttatct cgttccccag
     3541 gaaccgctgc ttttcccaag cctgtcgata aaagaaaaca tcctgtttgg gctggcaaaa
     3601 aaacagctct ccatgcagaa aatgaagaac ttgctggcgg cgctgggctg ccagtttgat
     3661 ctgcatagtc tggcgggatc gctggatgtc gccgatcgcc aaatggtgga aatcctccgc
     3721 gggctgatgc gcgactcgcg gattctgatc ctcgatgaac ctaccgcctc gcttacccct
     3781 gcggaaaccg aacgcttgtt tactcgcttg caagagctgc ttgctactgg cgtgggtatt
     3841 gtttttatct cgcataagct gccggaaatt cgccagattg ccgatcgaat tagcgtgatg
     3901 cgcgacggaa ccatcgcctt aagcggcaaa accagcgaac tgtctaccga cgacattatt
     3961 caggccatca cgccagcggt acgggaaaaa tcgctctctg ccagccaaaa attatggctg
     4021 gagttacctg gcaaccgccc acaacatgcc gccggaacgc cggtgctgac actggaaaat
     4081 ctgaccggcg aaggtttcag gaatgtcagc atgacgctca atgccggaga aattctgggc
     4141 ctggctgggc tggtgggggc cggacgcaca gaactggccg agacgctcta tggtctgcgt
     4201 actttgcgcg gcggacgcat tatgctgaat ggtaaagaga tcaataaatt atccactgga
     4261 gaacgtttac tgcgcggtct ggtttatctg ccggaagatc gccagtcatc gggactgaat
     4321 ctcgatgctt cgctggcctg gaacgtctgt gcccttactc ataaccttcg tggattctgg
     4381 gcgaaaaccg cgaaagataa tgccaccctg gaacgttatc gtcgggcgct gaatattaaa
     4441 tttaaccaac cggaacaagc tgcacggaca ttatccggtg gcaaccagca aaaaatcctc
     4501 attgccaaat gcctggaagc ttcgccgcaa gtattgattg tcgatgagcc gacgcgcggc
     4561 gtggatgtct cggcccgtaa tgatatctac cagctgttgc gcagcatcgc cgcacaaaat
     4621 gtggctgtgc tgcttatctc ctccgacctg gaagagatcg aactgatggc agatcgtgtg
     4681 tatgtgatgc atcagggcga aattacccac tctgcactga ccgggcgcga tattaatgtc
     4741 gagactatta tgcgcgttgc cttcggcgat agtcagcgtc aggaggcgtc atgctgaagt
     4801 ttattcagaa caaccgtgaa atcacggcac tgctggcggt ggtgctgctg tttgtattac
     4861 ccggttttct cgaccgccag tatttaagtg tgcaaacgct gaccatggtt tatagcagcg
     4921 cgcaaatcct gatcctgctg gcaatgggcg cgacgctggt aatgcttacg cgcaatattg
     4981 atgtttcagt gggttcgatt accggaatgt gcgcggtgct gttggggatg ttactgaacg
     5041 caggatattc actacctgtt gcctgtgtcg cgactttact gcttggtttg ctcgcgggat
     5101 ttttcaacgg tgtcctggtc gcgtggctaa agatccctgc cattgttgcc acccttggca
     5161 cgttagggtt gtacagaggc atcatgttgc tgtggactgg cggcaaatgg attgaagggt
     5221 tacccgccga actgaaacag ctctccgcac cgctgctgtt tggcgtttcc gcgattggtt
     5281 ggttgacgat aattctggtg gcatttatgg cctggctgct ggcaaagacg gcgtttggac
     5341 gcagttttta tgccacgggc gataatttac agggcgctcg tcaactgggc gttcgtacgg
     5401 aagccattcg cattgtggca ttttcgttga acggttgcat ggcggcactg gcggggattg
     5461 tgtttgcttc gcagattggt tttatcccca accagaccgg taccgggctg gagatgaaag
     5521 caattgcagc ctgcgtgctg ggcggcatta gtttgctcgg cggttccggt gcgatcatcg
     5581 gtgcggtact cggcgcatgg ttcctgacgc agatcgatag cgtactggtg ctgttgcgca
     5641 ttccggcatg gtggaatgat tttatcgcgg gtctggttct gctggcggtg ctggtgtttg
     5701 atggacgcct gcgttgtgcg ctggaacgta atctgcggcg gcaaaaatat gcccgcttta
     5761 tgacgccacc gccatccgtt aaacccgctt cgtcaggtaa aaaacgggag gccgcgtaat
     5821 gcgtattcgc tacggttggg aactggctct tgccgcactg ctcgttattg agattgtcgc
     5881 atttggtgca attaacccgc ggatgttaga tctcaatatg ttgctgttca gcaccagtga
     5941 ctttatctgc attggcattg tcgccctacc gctgacgatg gtgattgtca gtggcgggat
     6001 cgatatttcg tttggttcga ccatcggcct ctgcgccatt gcattgggcg tactgtttca
     6061 aagtggtgtg ccgatgccgc tggcgatact cctgacctta ctgctcggcg cattgtgcgg
     6121 gctgatcaac gccggattaa ttatctatac caaagttaac ccgctggtga ttacgcttgg
     6181 cacgctgtat ctgtttgccg gaagtgccct gctgctttcc gggatggccg gagcgacggg
     6241 gtacgaaggt attggtggat tcccgatggc gtttacagat ttcgctaacc tggatgtgct
     6301 gggactcccc gttccgctga ttatcttcct gatatgtctc ctcgttttct ggctctggct
     6361 gcataaaacc catgccggac gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct
     6421 ttatagcgcg attccagtta accgcacctt atgtgcgctc tatgccatga cggggctggc
     6481 gtctgcgatc gccgctgtgc tgctggtatc gtattttggt tcagcacgtt ccgatctcgg
     6541 tgcgtcgttt ttgatgcccg ccatcaccgc cgtggtgctt ggcggtgcca atatttatgg
     6601 tggttccggt tccattatcg gcaccgccat tgcggtttta ttagtgggat atttgcaaca
     6661 aggtttgcaa atggcaggag tgccaaatca ggtgtccagc gccctttccg gtgcgctact
     6721 tatcgtcgtt gtcgtaggtc gttccgttag cctgcatcgc cagcaaatta aagagtggct
     6781 ggcgcgtcgg gccaataacc cattgccata aaggatatct tcatgacact tcatcgcttt
     6841 aagaaaatcg ccttacttag cgttcttggc attgccgcaa tctctatgaa tgtgcaggcc
     6901 gcagagcgta ttgcatttat tcccaaactg gttggcgtgg gattttttac cagcggtggc
     6961 aacggcgcac aacaagcggg taaagagctg ggcgttgatg tgacctacga cgggccgaca
     7021 gaacccagtg tttctggtca ggtacagttg attaataact tcgtcaatca gggttataac
     7081 gccattatcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg
     7141 caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg ccgctcttac
     7201 tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt
     7261 caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac cgttacggac
     7321 caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa
     7381 attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac cgcagaagga
     7441 atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa tgccctgccc
     7501 gctgccgcac aagccgcaga aaacctgaaa aatgacaaag ttgcgattgt cggattcagt
     7561 acgccaaacg tgatgcgtcc atatgtggaa cgcggcacgg tgaaagaatt tggcctgtgg
     7621 gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt gaaaaaagga
     7681 tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggtgga agtctcgcca
     7741 aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact gttaccggag
     7801 cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc attacttaac
     7861 cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt cgtaccgatc
     7921 aaccgcaaca aaatatccct tttatcctga aaggttgcgg tgcgctggat tggggaatgc
     7981 agtcacgctt atcgcggata tttaatccga aaacgggtaa aaccgtgatg ctggcctttg
     8041 accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata aatatcgccc
     8101 cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc
     8161 ccccggcgac caataagccg gtggtactgc gggcgtcagg tgcgaactct attctggcgg
     8221 aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg
     8281 cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc aaaaatatta
     8341 ttcagctggt tgatgccgga atgaaagtgg gaatgccgac catggccgtg actggcgtgg
     8401 gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa
     8461 tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg attgttgccg
     8521 gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc gaggcgctgg
     8581 aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg cgtaatattt
     8641 tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt caccataacg
     8701 aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg cggatttaag
     8761 gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca aggttgacga
     8821 gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag gtaatttgcg
     8881 cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg aagcctataa
     8941 agatgaagac gcagtggcat tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa
     9001 actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg
     9061 aggcgaattc atcagtttta tctacaattg gggtaacgcg ctgacgggag taaataaatg
     9121 tctgactgga acccctcttt atatctgcac tttgccgctg aacgatcgcg tccggcggtg
     9181 gagctgcttg ccagagtacc gctggagaat atcgaatacg ttgccgatct tggctgtggc
     9241 ccaggtaaca gcaccgccct tctaaaccaa cgttggcctg ctgccaggat aacaggcatc
     9301 gactcgtctc cggcgatgat tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg
     9361 gaagcagaca tccgcaactg gcaaccggaa caggcactcg atctgatttt tgctaatgcc
     9421 tcactgcaat ggctgcccga ccactacgaa ttgtttcctc acttggtttc tttacttaat
     9481 ccgcagggcg tgctggcagt acagatgcca gataactggc tggagccgac tcatgtgctc
     9541 atgcgcgaag ttgcctggga acaaaactac ccggatcgcg ggcgtgagtc gttggctggc
     9601 attcatgctt actacgatat tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc
     9661 acttactatc accagatgcc gtcgcaccag gcgattatcg attgggtgac cgccactgga
     9721 ttacgtccgt ggttacagga tctggccgag agcgaacagc agctttttct tacgcgctac
     9781 catcagatgc tggaagagca gtatccactg caagagaacg gacagatact gctggcattt
     9841 ccgcgtctgt ttattgttgc ccgccgtacg gagtaattat cacgtcagct ggtaatgacg
     9901 atcgggaaga atttttgctg gaatttcggc ttcatcgttc atctgtaaca ggtcaatttc
     9961 aatagcgttg cagatggcat ccagcggtaa atcattgttt tcagtaccga acggatcttc
    10021 caattcttcc gccagacaat ccagcgaaat aaaagtgtag gaaatcagca cagagataaa
    10081 aggcgtcatg taatgcaggt ccacgaccag cgcgaacggc agcatgatac aaaacagata
    10141 aacggtacga tgcaaaatca gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc
    10201 acagcctgcc agaaccgctg aaatatcatt aagccgatcg ttgaggctaa taaacaggat
    10261 atctgaaagc tgcccattgc ggcgctgaac cgccaaccac tcccccatta ttaacaagat
    10321 acggttagct ggagagttcg aagccagtac aggctgaaga tcttcagtct tgagataatg
    10381 agccagcact tccgcctgtg gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc
    10441 gatttgcaac cgggcaaact cccttacact tgccgaatcc ggcaatgtcg tttttacctc
    10501 gcgcagtaac gaccgtgagg caatcatcaa ctgcccccaa agttttcgcg cttcaacgta
    10561 acgggcgtac ccggcattat tacgaaaacc aagaaaaatg gcgatggcga caccgagaat
    10621 gctgaacggt gcgagggtga atttgatgcc cagatgcgtg taccagggca gcatgaaaat
    10681 aacagcgata gaaaagagaa aattgagtag taagcgcgat gatatcttgg ataatactga
    10741 gccgtgccag acaaaaatac ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat
    10801 cttcaggcgt gaaaaagtcg ccctattaaa cgtgattacg atcacattct caagacgcta
    10861 cttacaaatt acctacaagc ttacaacatt aacaggcgct gcatgtggca tctgttaatg
    10921 ataaaggcac ataggatgtt gtaactaata tagtgaaata agaaacccgg tcgaaaccgg
    10981 gttcagaagt aacggtgtta ttagcacaac ggacgtacag cttcgcgcat ccctttttca
    11041 agaatcgcat ccaggtcatt agcaacctgc tcgaccagac caggcacttg cgtcaggtcc
    11101 tgctcccagt ggtctttttc tgccaaaaca atcgctacca gttcctgggt gccgataaca
    11161 cggtcacgat gctggctcca gagttgctga taacgttcca gccagtgtgc atcgtcctgt
    11221 accggatatg tttctccgtt acgctcaccg cgatagaagg caatcaaagc cgctaatgcg
    11281 aaagtaaggc gcgccggaag tgtgcctttt gccttctgcc ctgccagcag ctgcggcagg
    11341 atgcgagtgc ggaacttggt cataccgttg agcgcgatag acagcagctg atgcttaatg
    11401 tacgggttac ggaaacgccc ggtgactgca ctggcgaaag attccagttc atcacgaggc
    11461 aaatccagta ccggaataat ttcttcgtag atagcttttt caacgaatgc gcaaatttca
    11521 gcatcgttca tcgcttcacc tacggtatcc agccctgcct ggaacgccac cggcaccagc
    11581 gcggtgtgcg caccgttgag gattgccact ttgcgttctt tgtacggttt aatatcgtca
    11641 acaatcagca cgttgagcgg atatttgtcc agacgcagtt cagtcgctaa ggatttcggt
    11701 ccctgaatca caaacaggta aaagtgttca gcggtgtcga ggaaaccatc gtgataaccc
    11761 agttcttctt ccagtttagc cacttcatcg cgcggataac cggtaacgat acggtccacc
    11821 agcgtagaac agaagctgtt agcctgatcc aaccattgaa taaatgcttc tggcagtgcc
    11881 cactcttgag catagcgcag caccagttca cgcaacgcgt cgccattgta gtcgatcaac
    11941 tcacacggga tgatgatcca acctttatcc agcgcaccgt tgaaatggct aaaacgttcg
    12001 aacagcagac gggtcagttt tgcgggatag cttaccgctg gcgcgtcatc gaatttatcg
    12061 cccgcatggt agctgatacc cgcttcggtg gtgttagaga aaacaaagcg catttccgga
    12121 ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga cgctgatttc acgattaaca
    12181 gagcgaatca gacgcgcgtc gctgaccgct tcccccttct cattcaggcc acggataatg
    12241 gtggtgtaca gaccatcctg cgtgctcagt gacggcggga atgaagtttc aatcggacga
    12301 acaacgacca cgccagaatt cagatcggtg tgctcattca ggagatcgat ttgccagtca
    12361 acaaaggcgc gcaggaagtt accttcacca aactgaatga tacgttctgg atactgtgca
    12421 ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt cccttctgat tagtcataca
    12481 acctgtttga attggtacga caggttagca aactttaata cgccgaaccc ctgttttgat
    12541 caactcctga tgattaatga gcagttttat gagaaaagtg tggcgcggat catggtttaa
    12601 tcgaggaaat acgccttttc ctggatcata aagtggtaga acacattgca ttcaaatcgc
    12661 gcggaatgaa taaagatgtc agacaacttc ctcaccgtaa cgcatagtgc tggtacggtt
    12721 gcgcccatct ttcttcgagc gatacagaca cgtatcggct tcgaccatca atttattaaa
    12781 atcatcggtc agcgttcggt acgatgcgcg accactaccg acgccaatac ttaccgtgag
    12841 ataaagcgtt ttttgttgcc aggtgaatgg ttgcagttca acgcctttac ggattttttc
    12901 cgccattagc agaccatcga caggattcac cgacggcact gcaacagcaa attcttcgcc
    12961 gcccatgcgc gccaccagcc ccttatcacc gacaatcttc tgaatatgcc gggcaaacac
    13021 gcttaacact ttatcgccac attcatgtcc atagttatcg ttaatgcttt tgaaatagtc
    13081 aatatcaagc agcatgaccg tcagatgttg tgtctgtttc agcgatggac ttttcagcgc
    13141 ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa tcaaagtcgg ctcgcagcgc
    13201 aacttgcttc attagcgaat tgatcgctgc cacgctaaaa gaaaccataa ttgggcatat
    13261 cgccatcgtg gcaataccga gacgtgcgga gaacatttcc ggaatggaga acggcgaacc
    13321 gaccgagata tcaatcaccg aatttgccac cagtacgatt tccaccgcac cggtgacaaa
    13381 ggtcaacaga catgttacct gcggcgtata gcgcactgca caccagatta atgcaggcag
    13441 cgggaacgcc agactccccg ctccgccaat gactaccgag gcaataaccg acacaataag
    13501 cgcgatggcg ggcatcatct gttctgcttt aaagcgcggc agcactccag gaatagccaa
    13561 cgtcagcata caaggcacga tcaacacgcc cgttgagaat tgctcactga accagtccgc
    13621 aagcaaaggc cagaaatcca gactatcaat actgaccgaa ccaatcgccc cgacaatagc
    13681 gcataataat gcggcaatca gacagtaatt aaatagccgt aaggcactta ctggctcata
    13741 cttatttttc cccagacgct tatcccgcac gaccagtaag gcgacggtaa caataaacat
    13801 catattggag aaattgataa ccagtgaaac cagtccccat tcggtggtta tggcatcata
    13861 aaccagcatc gccacataac tgatcgcata gtaatgcagt cgattaagcc agacatagcg
    13921 ggcaaacacc cccgccatta cgccattcag cggccagaaa agagaaagtt cgtgaaccag
    13981 ccgtaactct gcaccaataa aatagaacag cgtggttagc gcaaaaatag cgatgctatt
    14041 acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa atggggtgta catgcattaa
    14101 ttatctgtcc atacaattgc gggtggcaat tatctcaacg taataagtaa attctcataa
    14161 aggatagcat gttcgctaac agcggcggca aataaaggta ccctgggaat gctattttat
    14221 cgttttccgc aattctcatg gctgattcat ctgtacaacc agccagtgat agattaccgc
    14281 cacgatataa cgttgttgtt cttcgcttaa cgaaacaccc tgcggaatgt tatgccactt
    14341 agccagacaa ccgcgacagc aggtcgcggt ggcgtgctga gcgataaaca ccggatgccc
    14401 gcgcatgggg gtttgcttac cgtcattagc cggtaatgcc ggagcaagac gtttagcgac
    14461 aaaatccgcc gcatgttgtt cgatgactgg cgcgcctttc tccaggcaat actgacgctc
    14521 tttcatgccc agacgaaagc gagagcgaaa ttttgaacgg gataaacgcg cgaagagtgg
    14581 atcgagagac tgcattaata aaccgaacgc cctaactgtt tcgtcaattg ttcaaggacg
    14641 gcaatacccg caagcgagtt acctgcatca tccagttccg gactccagac agcgatggcc
    14701 atttcatgcg gaacaatcgc cacaataccg ccaccaacgc cagatttcgc cggtagcccc
    14761 acccgccagg caaactcccc cgcgttctgg tacataccac tggtcgccat cagcgcgtta
    14821 atttgccgcg cctgcattgg cgtcaccaca ggttcatcaa tatgaatagc tttcccctga
    14881 ttagccagaa agacaaacgt ccgggccagc tctacacagc tcattttcag agcgcagtaa
    14941 tgaaagtagt tttgcagaac ggttgtcaca tcatgatgga aattgccaaa cgacttcatc
    15001 agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt cggaacgcgc taccaccgta
    15061 tcgtatgaaa tatcagacac accgcttaag ccgcgcacga cttccagcat acgttgccgt
    15121 ggtgcgctta atcgcccttg caacatatcg caaaccacca gcgcaccagc attaataaac
    15181 ggattacgcg gaataccctg ctccatttcc agttgcacta aagaattgaa cggtgatcca
    15241 gacggatctt tgccgacgcg ttgccagatt tcctcttcgg agtaatgacg catggcgaca
    15301 acgagactca gcactttgga aatagactga atggaaaaac gttcttgcgc gtctccggcc
    15361 tgaaaaagct gtccgtcaac ggtacagata gcaatgccca atcgggaacc gtctactgta
    15421 gccagcgccg gaatataatc cgcgacttta ccctgaccaa tgagcggccg cacttgccgc
    15481 aagatgtttt ctaaaattgc attatccatg gcgactgcca ctttctactc ctggaccgca
    15541 ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt cgcgtcagat ccggtctttc
    15601 cacaccgtct ggatattaca gaattcgtgt aagccgaaat gggaaagctc acgaccaaag
    15661 ccactctttt tcacgccacc aaaggccact cgcgcgtcgc tggcacaata accattgata
    15721 aacaccccac cgcattccag acgtgccgcc atctgtctgg cctgtgtttc gtcggtggta
    15781 aaaatggtcg ctgaaaggcc gaactcacta tcattagcca gttccagcgc atgttctgca
    15841 tctttcgcaa cggtgattgc cgcaacaggg ccaaacattt cttcccgaaa cgcggtcatt
    15901 tctggggtaa cattcgccag aaccgttggc ggatagtagt tacctgcccc agccatcttt
    15961 tccccgccca gtaacaaacg cgcaccctgc gccagggttt tctccacctg atgatgcagc
    16021 tcatcacgta aatcaaaacg agccattggt ccgagagcgt tctcttcatc acggggatcg
    16081 cccattttca aggctgccgc agctgccaca aaacgttcgg taaatgcaga agcaattccc
    16141 tcttcgataa taaagcgttt tgccgctgca caaacctgtc cggtattctg ataacgtccg
    16201 gctaccgccg ctttaaccgc cagttccaga tcggcatcgt taagcacaat aaatggatcc
    16261 gaaccgccca gttccagtac gcattttttc agtgccgctc cagcctgtgc gccaatagcc
    16321 gctcccgcac gaacacttcc ggtcaccgtg acagcagcaa tgcgcgaatc tttaatcatt
    16381 tgactgacac cgtcgttgtc ggcattcagc cagccatata cgccttgcgg gattcccgca
    16441 tctttaaaca cctgggcaat gagctgagca cagcccatca cattcggcgc atgtttaagt
    16501 aagtagccgt tacctgcaag aatgatggga accgcgccac gcatcacctg ccataacgga
    16561 aagttccacg gcataatcgc cagaatcgtc cccaacggtc gatactcaat aactgcctgc
    16621 tgattttcca ccagcgtagg ttccgccttc agcattgccg gaccatgttc tgcataccag
    16681 tcacacaaat tcgccgattt cgccacttca gcgcgcgcct gattgattgg tttgcccatt
    16741 tcacgggtga tcatttgcgc catttcttcg ctacgggcgc gcagagcctt accgatacca
    16801 cgcagttttt cagcacgata atctatattt gtctcgcgcc agtcgcgaaa gcctgctgcc
    16861 gccagctgaa gtgcgttttc gatatcgtta gcgccagccc acggcagcac agaaagttgt
    16921 tcacccgtgg caggatttat tgaaattgca tgagttgccg gagtaatggt catcggggta
    16981 tctcctttat gagtcatggt atgaagatac gcagatttac tcttgcttta aaatgaataa
    17041 tattaagcca cttattcacg aatcgagaat attatggatc tgacacaact ggagatgttc
    17101 aacgcggttg ccgaagctgg cagcataacc caggcagcag caatagtgca tcgcgtgccg
    17161 tcgaatctca ctacccgtct acgccagctg gaaacagaac tgggggtaga tctgtttatt
    17221 cgcgagaatc agcgtttacg tctctctcct gccgggcata actttttacg ctatagccaa
    17281 caaattctcg cgttagtgga tgaagcgcgg agcgttgtcg ctggcgatga gccgcaaggt
    17341 ttattttctc ttggttcgct ggaaagcacc gcagcagtgc gcattccggc cacgctggcg
    17401 gaattcaacc atcgttatcc caaaattcag ttttcacttt ccaccggccc ttccggcact
    17461 atgctggaag gagtgctgga gggaaaactg aatgcggcat ttattgatgg gcccattaac
    17521 catactgcca ttgacgggat accggtatac cgcgaggagc tgatgatcgt cacgccacaa
    17581 ggtcatgcgc cagtaatccg cgccagtcag gttaacggca gtaacattta tgccttccgc
    17641 gccaattgtt cgtatcgtcg ccacttcgag agctggtttc atgctgacgg tgccgctccg
    17701 ggaactattc acgagatgga gtcgtatcac gggatgttgg cctgtgtggt cgcaggagca
    17761 ggcattgcgc ttattccacg ctctatgctg gaaagtatgc cggggcatca ccaggttgaa
    17821 gcgtggccgt tagctgagca atggcgttgg ttaacaacct ggctggtctg gcgtcgcggt
    17881 gcgaaaacgc gtccgcttga ggcatttatt caactactgg atgtgtctga ctcggcaaaa
    17941 cagagttacc aatgagctat tttgatagtt cttgcggcta atatgctcta tatagtgatg
    18001 ttccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca tcagcaatta
    18061 catatctctt cctgatgatt ttcccgttcg taatattgca ccacaggtaa aagaagtttt
    18121 aaaggacttt attgatgcgc taagtacaat aatatgtgat gaagagtggc gcacgtcttt
    18181 aaacatcaac tcagccacaa aaaa
//
LOCUS       DAMZII010000097        17615 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZII010000097 DAMZII010000000
VERSION     DAMZII010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.097"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            191..841
                     /gene="csgD"
                     /locus_tag="POA77_003270"
     CDS             191..841
                     /gene="csgD"
                     /locus_tag="POA77_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDI6366654.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            846..1235
                     /gene="csgE"
                     /locus_tag="POA77_003271"
     CDS             846..1235
                     /gene="csgE"
                     /locus_tag="POA77_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDI6366655.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1260..1676
                     /gene="csgF"
                     /locus_tag="POA77_003272"
     CDS             1260..1676
                     /gene="csgF"
                     /locus_tag="POA77_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDI6366656.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1703..2536
                     /gene="csgG"
                     /locus_tag="POA77_003273"
     CDS             1703..2536
                     /gene="csgG"
                     /locus_tag="POA77_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDI6366657.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2600..3091)
                     /locus_tag="POA77_003274"
     CDS             complement(2600..3091)
                     /locus_tag="POA77_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI6366658.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3193..3747)
                     /gene="ycdY"
                     /locus_tag="POA77_003275"
     CDS             complement(3193..3747)
                     /gene="ycdY"
                     /locus_tag="POA77_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDI6366659.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3771..4508)
                     /gene="ycdX"
                     /locus_tag="POA77_003276"
     CDS             complement(3771..4508)
                     /gene="ycdX"
                     /locus_tag="POA77_003276"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDI6366660.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4563..5501)
                     /gene="ghrA"
                     /locus_tag="POA77_003277"
     CDS             complement(4563..5501)
                     /gene="ghrA"
                     /locus_tag="POA77_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDI6366661.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5735..5822
                     /locus_tag="POA77_003278"
     tRNA            5735..5822
                     /locus_tag="POA77_003278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5769..5771,aa:Ser,seq:gga)
     gene            complement(5972..6814)
                     /locus_tag="POA77_003279"
     CDS             complement(5972..6814)
                     /locus_tag="POA77_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6366662.1"
                     /translation="MSAITLSHPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISSMTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEVSEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGHQVDRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6899..7096)
                     /locus_tag="POA77_003280"
     CDS             complement(6899..7096)
                     /locus_tag="POA77_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDI6366663.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQQQLLQQNR"
     gene            complement(7113..7601)
                     /locus_tag="POA77_003281"
     CDS             complement(7113..7601)
                     /locus_tag="POA77_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366664.1"
                     /translation="MNLSLTPEADSINMQALNMGRIVVDVDGVNLAELINKVSENGYS
                     LRVVDKSDQQSTSTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(7598..7981)
                     /locus_tag="POA77_003282"
     CDS             complement(7598..7981)
                     /locus_tag="POA77_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6366665.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDLPGFSAGAPSSQLINSIDILRARRAT
                     GLMTRDNYRMVNNITLGKHPEEAKQ"
     gene            complement(8058..8438)
                     /locus_tag="POA77_003283"
     CDS             complement(8058..8438)
                     /locus_tag="POA77_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDI6366666.1"
                     /translation="MSDTLPGTTLPDDNKDRPWWGLPCIVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYLAVYPATETESNPPE"
     gene            complement(8449..9132)
                     /locus_tag="POA77_003284"
     CDS             complement(8449..9132)
                     /locus_tag="POA77_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDI6366667.1"
                     /translation="MSRIITTTVYTLNELSSTAQEKARDWYRQHHADSNWYENVYGDF
                     REVCGIFGIDLRQRVIRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARRVG
                     VYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYCMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAGCPVI
                     VKLSFTDFL"
     gene            complement(9151..9372)
                     /locus_tag="POA77_003285"
     CDS             complement(9151..9372)
                     /locus_tag="POA77_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6366668.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMNVTLN"
     gene            complement(9435..9911)
                     /locus_tag="POA77_003286"
     CDS             complement(9435..9911)
                     /locus_tag="POA77_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDI6366669.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9927..10412)
                     /locus_tag="POA77_003287"
     CDS             complement(9927..10412)
                     /locus_tag="POA77_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6366670.1"
                     /translation="MTTVLHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(10504..11322)
                     /locus_tag="POA77_003288"
     CDS             complement(10504..11322)
                     /locus_tag="POA77_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDI6366671.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11649..14495)
                     /gene="ag43"
                     /locus_tag="POA77_003289"
     CDS             complement(11649..14495)
                     /gene="ag43"
                     /locus_tag="POA77_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDI6366672.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTPVPALAADTVVEAGETVNGGTLTNHDNQIVFGTTNGMTISTGLEYGTDNEANTGGQ
                     WVQDGGTASNTTISSGGLQFVGAGGKATDTIINEGGGQSLKGLALNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVTVEGTANTTVVYAGGDQTVHGHALDTTLNGGNQYVHNGGTTSDTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFAVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTQDAVLSRAVAKGDAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLESDLLRTEVAGMSVTAGVYSAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIMAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHMRAGFRLGSHNDMSFGEGTSSRDTLRDSAK
                     HRVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(14867..15739)
                     /locus_tag="POA77_003290"
     CDS             complement(14867..15739)
                     /locus_tag="POA77_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDI6366673.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRVVRDT
                     VVSVARAVWNWIFF"
     gene            16003..17574
                     /locus_tag="POA77_003291"
     CDS             16003..17574
                     /locus_tag="POA77_003291"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6366674.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQVMTPTY"
BASE COUNT         4280 a         4600 c         4443 g         4292 t
ORIGIN      
        1 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
       61 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      121 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      181 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      241 taaaccgtct ttgcaagcga cagctctctt gcagcacctt aaacaatcgc tggcaattac
      301 aggaaaatta cataatattc aacgttctct ggacgatata tcttcaggct ctattattct
      361 tctggatatg atggaagcag ataaaaagct tatccattat tggcaggata ctttgagcag
      421 gaaaaacaac aatatcaaaa tattgttgct aaatacgcct gaagattacc cgtaccgcga
      481 cattgaaaac tggcctcata tcaacggcgt tttttatgcc atggaggatc aagaacgtgt
      541 tgtcaatggg ttgcaaggcg tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag
      601 ttacctgatt acacattctg gtaactatcg ttataacagc acggaatcag ccctccttac
      661 tcatcgggaa aaagagatcc tgaataagct gcgtatcggc gcgtctaata acgagatcgc
      721 tcgttcgttg ttcatcagcg aaaatacggt taaaacgcat ctttataatc ttttcaagaa
      781 gatagccgta aaaaaccgga cacaagcggt ttcctgggca aacgataacc tcaggcgata
      841 aagccatgaa acgttattta cgctggattg tggcggcaga atttctgttc gccgcaggga
      901 atcttcacgc cgttgaggta gaagtcccgg gattgttaac tgaccatact gtttcatcta
      961 ttggccatga tttttaccga gcctttagtg ataaatggga aagtgactat acgggtaact
     1021 taacgattaa tgaaaggccc agtgcacgat ggggaagctg gatcactata acggtcaatc
     1081 aggacgttat tttccagact tttttatttc cgttgaaaag agacttcgag aaaactgtcg
     1141 tctttgcact gattcaaact gaagaagcac taaatcgtcg ccagataaat caggcgttat
     1201 taagtacggg cgatttggcg catgatgaat tctaaataaa aaattgttcg gaggctgcaa
     1261 tgcgtgtcaa acatgcagta gttctactca tgcttatttc gccattaagt tgggctggaa
     1321 ccatgacttt ccagttccgt aatccaaact ttggtggtaa cccaaataat ggcgcttttt
     1381 tattaaatag cgctcaggcc caaaactctt ataaagatcc gagctataac gatgactttg
     1441 gtattgaaac accctcagcg ttagataact ttactcaggc catccagtca caaattttag
     1501 gtgggctact gtcgaatatt aataccggta aaccgggccg catggtgacc aacgattata
     1561 ttgttgatat tgctaaccgc gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg
     1621 gacaaacctc gaccatccag gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc
     1681 cagcttcata aggaaaataa tcatgcagcg cttatttctt ttggttgccg tcatgttact
     1741 gagcggatgc ttaaccgccc cgcctaaaga agccgccaga ccgacattaa tgcctcgagc
     1801 tcagagctac aaagatttga cccatctgcc agcgccgacg ggtaagatct ttgtttcggt
     1861 atacaacatt caggacgaaa ccgggcaatt taaaccctac ccggcaagta acttctccac
     1921 tgctgttccg caaagcgcca cggcaatgct ggtcacggca ttgaaagatt ctcgctggtt
     1981 tataccgctg gagcgccagg gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc
     2041 ggcacaagaa aacggcacgg ttgccattaa taaccgaatc ccgctgcaat ctttaacggc
     2101 ggcaaatatt atggttgaag gttcgattat cggttatgaa agcaacgtta aatcgggcgg
     2161 tgttggggca agatattttg ggatcggtgc agacacgcaa taccagctcg atcagattgc
     2221 cgtgaacctg cgcgtcgtca atgtgagtac cggtgagatc ctctcctcgg tgaacaccag
     2281 taagacgata ctttcctatg aagttcaggc cggggttttc cgctttattg actaccagcg
     2341 cttgctggaa ggggaagtgg gttacacctc aaacgaaccc gttatgctgt gtctgatgtc
     2401 ggctatcgaa acaggggtca ttttcctgat taatgatggt atcgaccgtg gtctgtggga
     2461 tttgcagaac aaagcagaac ggcagaacga cattctggtg aaataccgcc atatgtcggt
     2521 tccaccggaa tcctgacaga taaaaaagcc ggaattatca tccggcattt tactgtttaa
     2581 gcgaaagcgt gagtgcccct cacgctttat ttttgatttc ctgctcacgg tgagccgtct
     2641 ttgcactacg cgccgccagc cacaggccgc tgtttttcat tgcgtaacca aataccagcc
     2701 caagcgccag cgaaggtaac accagtttcc agtcaccctg cccggcaaac gtcgcgcatg
     2761 cgcctataaa cgtccccgga acaaatgaaa gcagcagctg tttggcctga atacacatca
     2821 gaaacgcgac aataccggtt atgacataac cgagaatttc cagatgtggt gccagtgcac
     2881 taccgtaaat aatgaccatc gcccacacca cgccactaag cagggttgca gcggagatcg
     2941 ccagcccttt cagcccacct tgcgggcagg caaagtaggc cgtacagccc aggaagcccg
     3001 cccagcttag taagcccagg gaaacagcca cccatcccca gataccggag agaatgcctg
     3061 ttgtgattgc aatagagaga agtatgttca tgccgcgcat gatagcagaa aacgcggcca
     3121 tgaatgagat cgagcacaca ttttatgcaa caacaaatta tctgttgcag ttatgagtga
     3181 cgatgatcac atttactctt cagaatcttc ttccagctcg tcccacattg cactaatggc
     3241 atcacgggtt agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt
     3301 gccgaggaac gcaccacacc agggtaacag atactcactg aacagtgttt ccagtgcttc
     3361 gctctcatct tccgttgact gatcttccag ccaggaagcc gcaagcagca atgtgccgat
     3421 gtgatcggct ggcgtatccg ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc
     3481 cgcttccgtt gcgtcctcaa cccatgcgct acgatatggc ggcacggcac attcatcgcc
     3541 gataaacaac gcattgtaat cggcagagac ttgcgccata tcacagcttt tctgtaaacg
     3601 cgtcagtaac tcatcctgct ccagtggcca gttcgcagcc agtttcccct cacgaatcag
     3661 ggtaaacagc ggcaccagta aaggatcttg cggttggcgg taatagagcg aacccagcac
     3721 acgacagagg atagaaaact cgttcattaa tatatttcca ttactagtaa ttaaaggtct
     3781 gcaaattccg caatcggtgc cataccgcga gattcaagga agttcagtaa gcggcgcggc
     3841 gaaacattca aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt aagacactct
     3901 tcaaattccc ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac ccaacctcca
     3961 gcatcacgta ccgctgcggc taccgcacga cagttgtctt cactgccctt acgtgagtgt
     4021 aaaaatgaag aattattgat ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg
     4081 gctttcacat ctatttcata tttgggattt ccgggatggc ttattatatg cacattgccg
     4141 ctggcgatag tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg tgcaaaaacc
     4201 ggctcatgaa aaccggcaat aatcagatcc agcgagtcaa acattttacc gctgcaatca
     4261 atttcaccat caacattttt aatgttagct tcgatgccgc gcaggatccc taccccatca
     4321 accactcgcg gccagatacg catgttaatg aagtgccagt gatgcggagc atcttccata
     4381 tccgggccat gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg
     4441 taatcactca atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg
     4501 ggatacataa ctctctcctg tattcaattc ccgccaagga tagcaggaat cctgatgctt
     4561 tattagtagc cgcgtgcgcg gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg
     4621 gcaatggtgc gagaaatgta ctccacagct tcagcgggac gggtaatcgc ggcgacatgt
     4681 ggtgttatcg tcacgcgtgg atgttgccag agcggacttt caggcggtaa gggttcacga
     4741 ttaaaaacat ccaacattgc gcctttaact ttgccgctat ccagcgccgc gagcaggtca
     4801 tcttccacaa catgaacacc acgcgccagg ttgaggagat acgcgccatc cggtaatttt
     4861 tcgagtaatt gttgattaat aatgccgacg gtttcagggg tattcggtaa caaattaatc
     4921 aatacccgac attggctcag aaatgcagac agttcttccc gtccggcaaa gctttgcacg
     4981 ccaggccacg atttacgggt tcgactccag caacgcagcg gaaagcgcca ggtttgcaga
     5041 ctctgagcaa ctttactgcc cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc
     5101 cgatgatatt caggcagcgg ttgccaatgc gaactatttt gctggatgcg ataatcgtca
     5161 aaacgtcgaa accaatgcag cacctgactg acagcatatt cctgcatttg ctcgcccata
     5221 ccggtatctt ccaggcgaaa aagtggaaca gaagggttca gcatttcagg gtgtgcctgt
     5281 agcttgctca aaatagaatc aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc
     5341 cctgccagca tttcaacagg aggatgccag actaaagcat aatcagcaga gtcattatct
     5401 ccgcttttcc acgctctgac tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac
     5461 cattgggtat cgaacgttgg gtgataaaag atgatatcca tactgactcc cgaaaagcgt
     5521 tgtgcgaaat ttattcgcac ttatcgttat gatctacaaa ggccaccagc ataacaaatc
     5581 cgtggtcggt ggcaaaaaaa gcagatttcg cttattaaaa ccacacattg attgaagttt
     5641 gaataaacgc gcgatttttt caaaaagttt gttgacctca ggtcatgatt tccctaaatt
     5701 agcgcccgtt ccagcaagac aggaacgaca atttggtgag gtgtccgagt ggctgaagga
     5761 gcacgcctgg aaagtgtgta tacggcaacg tatcgggggt tcgaatcccc ccctcaccgc
     5821 cagattataa gcgcatgtta ttgtttaaca tgcgcttttt tatttatcct tccggacagt
     5881 atgccatcgg gtatgccatc tgaaaccggt ttatatgccc cgcgatcata tcttatcgga
     5941 caatttccga tctttgagca tactggcaaa gctatactgc cagcatgtca ggatagtgtt
     6001 ttgcgataat atcattcagt ctgtcaacca gttctggttt cctgaacgtg atgtgtgcgg
     6061 agcccttctt aaagtatctg atgctgaaca tctcatcttc atagtcctct ttaccctgaa
     6121 cagactggat gtggtcatcc agacgaaggg tgatgttttc ccggttgtcg gggatcggtt
     6181 tgccgctgaa caggtgcagc attctttcca ggtcggcaag tcgatctacc tggtgcccgg
     6241 tgataagatg aaatccccac cggtcccatc tcaccagatt gttgacgata attttactgc
     6301 caaatttaca ggggcaatta gttttgtaat tccagctcaa tcccctgaag acgttgatca
     6361 cccctcgctc aaacacttca tctttgttct ggtgcagttg ttcaaatgtg ctcagaatgt
     6421 tcgcttcact gacttccgga aaattatcat actccagtga ccggtaccat gtatcacggg
     6481 cctgtgcatc cattaaggaa agcatccctg aacgttgcat caggtcacgc cagatttctc
     6541 ggtcaagatt ccgggtaatg gctttcattg ctgtttcggg tttctccatc agccagcaac
     6601 cgcagcgaaa atcctgtttc atggcccagt ccggggcggt tttacctcca atactttctg
     6661 tcattgatga gatttcagcc agttgccgga taagtgtgtc gatttgcagc agtgtactct
     6721 ttcgtacagc cagaatgtgg ctgactgagg ttgagcagat aacgtccgta tgcccgttga
     6781 ccacttccgg atgggagagt gtgattgcag acataattga tattcctttt ggtttataaa
     6841 cgaaaacgcc agccgtattc aggctggcgt cagtggaatg aagtctggtg agtaagactc
     6901 acctgttctg ttgcagaagc tgctgttggc gcagggtacg gatatcttcc ctggcctgtt
     6961 caatgcaggg cagcagtgct gctgaatccg ttttatcctc atcgttgtcg aagataatcc
     7021 ctgattcgca gtcaatattg tcctgcagcc acgcaatcag aatatccagc gccgtttctg
     7081 tggttaatga tttcatgttg tgaatttacg ggttaccagt cgaaagtggg gaaccccggc
     7141 agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg ggtgagagtg
     7201 acgaccagac gacggaacgt tcttgacagg cccaggcgtt taagccgcag aaccgggtac
     7261 gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca ttctgtttcg
     7321 ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt ttccgtgata
     7381 tgtgcggtac tgcaacgtat gccggtaagg gttgttaacg gtggtggtgt actggttgac
     7441 tgctggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac cttgttaatc
     7501 agttcagcga gattaacacc atcgacgtca acgacaatgc gccccatgtt cagcgcctgc
     7561 atgttaatgc tgtcggcttc cggcgtcagg gaaaggttca ttgtttcgcc tcctccggat
     7621 gcttgcccag ggtaatgtta tttaccattc tgtaattgtc ccgggtcatc aggccggtcg
     7681 ccctgcgagc ccggagaata tcaatgctgt tgattaactg agacgacggg gctcctgcgc
     7741 tgaatcccgg caggtcagtt cgtaccagca cgtatttttc aacgagaaag ttcaccgcgt
     7801 cacacagtga aatccctgcc tcaatatgct gctcaatcac acgttcatca gcgaacggtg
     7861 tgtcattcaa cgtaaggcca tagtgctggt ccagcagtcg ggtgagcagt gtctgccaga
     7921 tttcaaccgg agacgggcag cgcgatgccg cccgtacgtg tgtgtcgggt aatgttttca
     7981 tactgaaggt tttcctgata tgcagagatg aaaaatggaa aagtggcgtg gtgaaagcac
     8041 caggccggag cagaaggcta ttccggaggg ttactttccg tttcggtcgc aggataaaca
     8101 gccagataaa cgtaaccaca ggagccgagg gtgtcggcat cgcaggtcag ccctttcgca
     8161 tacagcgtga cggtatgctg atgccgggga ttcagttcac cgctggtgag catgagttcc
     8221 agttgtttca tcagcagtgg aaaagcctgg tccagatggt atgcatccgc attgctgaac
     8281 cggcctctga taccggcgcg gtctgcaagg taatgcaacc ggttaccctc ctgcaccaga
     8341 cgtgccccga aacagggcgt cacgatgcag ggcagccccc accaggggcg gtctttgttg
     8401 tcgtcgggaa gcgttgtccc ggggagtgtg tctgacacga taaaatcctc acagaaaatc
     8461 ggtgaatgat agtttaacga tgacggggca gccggcgcgc agaccggctt cggtgaaggt
     8521 atattcgttg atgagaaggg cctcatccac agcggcgtct gaggtcagcc agtcgtactc
     8581 gttttccagt tgacggtaca accagaaggc caggtcgcgc agggcttcgc tgactgcggc
     8641 ttccgcatcg gttgtcatag cctgaccagt cggactgttc cgggtgacgg tgatatccat
     8701 gcagtacgga tggcagtaac gtccccggtg gtggatttca gcctgcagtt gccagaagtt
     8761 acgcttctgc accgcctgca gggcatccgc gatacgatga agctcacggt cctgcggggc
     8821 atacaccccg actctcctgg ctgttgctga ctgccagtgc cagcgtcctt caaaacaggc
     8881 tccgtccccc tggctgcaga atccgctgaa ccagatgcac ggctcttcca taaacctgcc
     8941 gttgctgagc cggataacac gctggcggag gtcaatcccg aaaatgccac agacctccct
     9001 gaagtcccca tacacgtttt cataccagtt gctgtcagca tgatgctgac ggtaccagtc
     9061 gcgggctttt tcctgtgcgg tggaggatag ctcattcagt gtatacacag tggtggtgat
     9121 aattctgctc atcagagtgc ccttactgac tcagttcaga gtgacgttca tcagccggac
     9181 atacgggcca aaactgtcct tacggcgttc agcaaacacg gccagcacac cgggaatatc
     9241 ctgcacttca cgaccggtat acgcctcagc actgccgtgc cagcggtatt tgccggtaca
     9301 gaaaggaaaa agacgggatg ccggatgttg ttggtgaata cgcatggctt caccacgggt
     9361 gatgattttc atagtgtgat acctctgaag acagaagata aaagtgcaaa caagcgtgat
     9421 gtggttgtga cgggttaaag cagaccatgt tcggcaaagg agaaaacctg gttgccaccg
     9481 actatcagat ggtccggcac ccggatatcc accaggccca gtgcctgtac cagacgttcc
     9541 gtgataaggc ggtctgcctt gctgggtgtg acttcaccgg acgggtgatt gtgcgccagc
     9601 accacggcag cggcattgtg gtacagggcg cgtttaatca cttcccgggg atggacttcc
     9661 gtgcggttga tggtgccggt gaagagggtt tcaccggcaa tcagttgatt ctggttattc
     9721 agatacagca cccggaattc ttcacgctcc agtcccgcca tgttcagaat cagccattcc
     9781 cgtgccgcac gggtggaggt gaaggccacg ccgggttcat gaagatggcg gtccagagtt
     9841 ttcagggccc gcagaatgag actgcgctcg ccgggcgtca tctctccggg cagaaaggaa
     9901 agctgctgca ttgtccctgc ctccattcag tcgatgatgc gcataatggc gctgcattcc
     9961 ggatgctgca gggcgtaatc ccgcagccgg taataatgga ccgtcatggc ataacactcc
    10021 gtacgacagg cgtgatgact gtacgtcatc agacaggcag caatgccggc agcttccgga
    10081 ctcatttcag cgcggttacc gttcatggca ttgaacagta cccatgtttc gtcatcatcg
    10141 tcatccggtt cgggtgccat aaatgccccg ccgttgttca gggtgtacag attccagata
    10201 ccaccgcagt agtcttcaca cagacggtcc atccagccga agacacgggg ctccagggcc
    10261 acccactgtg gaatgaggcc aaagtgctgc ggccagaagc tgatgcgctg ttcatcaggg
    10321 actatggtgg caaccagctg aggctggtca ttccctgatg cagcggttac ggaaacagaa
    10381 ggagtggtgg aattatgcaa gacggttgtc atgagattat tccttataaa aagtaaatga
    10441 atggaagaaa tcccggggga agggacagac gtgagtcaga actgcgcttt cagggaaacg
    10501 gcatcagcgc atactctcca gcagcgtttc agccatcacc cacaatgcgc ggttgagctt
    10561 aatgtcggtg tcgatgctgt gaatggcacg ggtatggata cgttttcctc tggcactgcg
    10621 accggaaatc ccgcctttca gcatattctc ctgaatggtc tgataagcac tccacaggtc
    10681 cttaccgtaa tcctcccggc gtcgtggcgt cagaatgtcg gcggtggtga cgggccgatg
    10741 ttcgtcacca taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg ccggtggcgg
    10801 cagaatcagc gactgcatgg catcacgctt ttcctcaatc cggtcaaaaa cccccaccac
    10861 ctcgtaagcc ccttcaataa ctttctccac cacatttccc cggtgcggaa cacgcacttc
    10921 ccccagagac tgaccacaga cgcatccgtt ctgacagacg aacctgaagt aacccggcag
    10981 catctggtag ctggaggtac cgtcatggga gttgagcaga atgatttcag ggacatgttc
    11041 tccgtttatc tctccggccc gccgcagacg cagcatatgt tttgtgtatc cccggcggcc
    11101 cgggtcgcgc acacgggtct ggcaggcgaa gaatggctga aagccttccc gctgcaggct
    11161 ttccagtacg gtgatggtgg gaatgtacgc atatcgttta ctgcgggaag tatgccggtc
    11221 ttcaccgaaa atactgggaa catagtgcat cagttcttcg tgtgtcagcg gacggtcacg
    11281 gcgtatctgg ttcgtataac caaaacgact ggctagtcgc ataatttgct ccttatgaat
    11341 gagttagcgg tataaatgaa aaagccgcgt ctcccggaga agacacggcc tgacagatga
    11401 aatgaatgac gtttattgtc tgagaagccc ttcactggcg agctgcgtat ttttatggaa
    11461 ggagatcacc caataacccc atcgggatcg ggtaataacc ggccagactg tgatcgattc
    11521 agatgccatt tggcagcaaa agagccttcg gcaataaacg tgatgtacaa cccggcatat
    11581 ctgttactcc ctgaaagtaa aaggccccgg tcattatgac cggggcctga aggggagtga
    11641 cctgattatc agaaagtcac attcagcgtg gcctgaccgt tatagccttc ggcgctgctg
    11701 ccgctgacgc tgtgggcata accggcctga acgcccaggg tgatattttc ccggacacgg
    11761 gcttccagtc cggcctgcag gtccagtgac gtgccattct gggacggtga gaacgtcatg
    11821 ttactgccgg cggcggctgt ccccatgctc atgtcacccc gggaactgaa ggtgcggata
    11881 acagaaggct gtacccacca gttcaccggc agttcacgca cacggtgttt tgcactgtcg
    11941 cgcagggtgt cacgggatga ggtgccttca ccaaagctca tatcgttgtg gctgcccaga
    12001 cggaagccgg cacgcatatg ttgtgcactg ccatgcccga acttcacata accggcgtta
    12061 tcctggccgt catccaggga aagtccctgc caggtgtact gcagttgtgg ttccagcatc
    12121 aggttgtcag tgatactgaa gggcagaccg gtttccagtg agcccagcca gccccagccc
    12181 cgggcgcgga agtcgttatt gtccgatgac gctttcatgc tgtggcgggt tccctgtgcc
    12241 ataatgtcag cccacaggcc ggaggacgtg tgtaccagat tcaggtatcc gcccaggctg
    12301 ccggcatcat cccggaccgt gccggcgcgg gagccgtcat catccttaac atcaacggaa
    12361 gaatggccag cagcactata tacccccgcg gtcacagaca taccggcaac ctctgttctc
    12421 agcaggtcac tctccagacg gacgaagcca tagctgccgc tgctttccgg cgtggcccca
    12481 cgggcaatac cgccgttgtt atcgtgaccg agatgaccgc cctgaatgct gagacggacg
    12541 ctgttatttt caccgtttac accggtctga tggctgcggg agcctgccag aatccggtca
    12601 tagtccattg cctgtgtcag catggaggca tacaggggga cttcagcacg ataagcattt
    12661 tcactgcgca ggtaccagtc ttcatcgctg tcacggttca gggtgtagtt aaaggcaccg
    12721 gcctgaagcg ggcgactcag ggcaaacgca ccttcttctg tggtggcacc attctgtgca
    12781 tccacaaccc ggataccctg tccggaagtt gccaccccga ggttactgtt tccgacattt
    12841 gtgaacgcaa gccaggtttt gccggttgcc tgaccaccat taatcaccag ctggtcagag
    12901 gcattgctgc catcaaggcg aacacgcata ttgatggtgc caccctgacc ggtgaggtta
    12961 ctggtggtca gtttatggaa cgttaccggg gcgtcgcctt ttgcaacagc acggctcagg
    13021 acggcatcct gtgtcgtctg attatcaaag cgaatctccc ctgcgttaac aatatcgccg
    13081 gtactgacca catcaccatt cagtaccatg ctggcgcctt tactgagctg tgttctgcca
    13141 ctcagacttc ccccggcagc cagcgtcagt gttccacgat gtccgacagt ggtgttgcca
    13201 gcctgtcccc cggcattaac cgtaaagctg ccgccatttt ccagcagcag gccgctggcc
    13261 tgaccggata tgccatcaat actgaactta ccgctggcat tggtcccctc aacagtggca
    13321 ccactgtctg caatcagggc accaccggat gtcatggtga cacctgtcgc cttaccaccg
    13381 gcagacactg ccagggtacc gccgtcatcc acccgtgttt tctgcgctga atgaccctcc
    13441 agtacatcca gacgaccgcc ggactccaga acaacaccgt cagccttacc gttttccacc
    13501 gcgaaattgc ccagacggtt gctgccggtg acggttgccg ccgtactggt gaccagtgcg
    13561 cctccctgct tcagggtgac attcgtggct gtaccaccgg cgttcacctg cagtttgcct
    13621 ttctggttaa cggtggtgaa atccgccaga ccaccttcct tgataatctg ccagccgtca
    13681 ctgttcacaa cagtgtcaga cgttgtaccg ccgttgtgta catactggtt accgccattc
    13741 agcgtggtat ccagtgcgtg gccatgtacc gtctggtcgc cgccggcata aaccacagtg
    13801 gtatttgctg ttccttcaac agtcacaatc tgacgaccat ttttattgat ggtggtacgt
    13861 acggcatttc cgcgaacaaa ctgcccggta tcaccatttt ctgcatccgg tcctccttct
    13921 gcgccggtat tcacaacggt gtctgttgcc actgcgccgg gtttgacgac ctgccagccc
    13981 ttatcattaa tgacggttcc tgtggctatc gcaccttcat gcatccactg ctcgccacca
    14041 ttcagcgtgg tgttcagtgc cagtcccttc aggctttgtc cgcctccttc attgattatt
    14101 gtgtctgtag cctttccccc ggcacctaca aactggagcc cgccgctgct gatggtggta
    14161 ttactggctg tgccgccatc ctgtacccat tgcccgccgg tattggcctc gttatccgtc
    14221 ccatactcca gcccggtact gatggtcatt ccgttggtcg taccgaagac aatctggttg
    14281 tcatgatttg tcagtgttcc gccgttcacg gtttctcccg cctctacaac cgtgtcagca
    14341 gccagtgccg ggactggcgt gacagcagca agagacagcg caaccgccac accggcgcgt
    14401 tttccccgtg agcgggccag ttcggaagcc accaccaggg tgcccgtaat gtgattccat
    14461 accagcctgt agctggtgtt cagatgtcgt ttcatcagct tttccttaca gagggtgaat
    14521 aaaaaagccg gtacccacaa atgtggatac cggcaggtac aaaacagcat ggtcagataa
    14581 agtggttttt cccggacata aatgcttccc ctctctcatc cgccagagaa gtatcagtcc
    14641 gttgtgtgca attccgttct gcatgatgaa gagattcagc tgccttttcg cagaccatta
    14701 accaccataa acgatcgatg acaacgatcg atacctgttt tatcgatcgt tttattctat
    14761 taacagcaag gcagtcacgc aagagaaaga ggtgggtatg aggtgtttgt atcatgtttt
    14821 cagttggtta acgcaatgta atcaggcatt tcccctggca gaatgttcag aagaaaatcc
    14881 agttccatac agcgcgggca acagagacca ccgtgtcacg gacgacacgc agaaccgtac
    14941 gagccacaga ggcaacacag acgctctccg ccgtgtcaaa tatccggtcc accgcaccgg
    15001 taaactgttc acgggcctgc ccccggacag attccgtgca cagctcgtcc tgcaggcggg
    15061 tcatcagggg actggcggca tggtcgggaa gtgctgtcat gagcgcactg accagcgtat
    15121 ccagttccca gccggtgcgg gccgatacgg ccacaaccgg atgtacgggc cggaacagac
    15181 ggaataccgc ctccgttttt tcgcgaatgt tctgtgcctg tgcgggagaa ggctgaatac
    15241 cggccatatc ccattcatgg cagggctccg ttttatcggc ctgcgtcacc acaaacagca
    15301 cctgctgatg tccccggtgc aggatgtgtc gccagaaata ctcatccaca gacagggcac
    15361 ggtcatcggc tttaatcagc cacagtacca ggtcaagttc aggcagaatg tcacggtaca
    15421 gggcttcata ctctgcatcc ctgtcccggc tctcgcccac cccgggcagg tcagtgataa
    15481 ccatgctgtg accgtggcca ctcagacgga agcgccgcac ttcccgggtg ccggcgtgaa
    15541 catcactgac cggggtgacc tccccctgaa acagtgcatt acagagtgag gatttaccgg
    15601 ccccgctttt ccccataatg ccaatcacgg gttcgtgact ggtgagttta cgcagatgtt
    15661 ccaggatgtg acgggaaagt gagtaaggca gggaagaaag tggtttttca attgcctcaa
    15721 tggcatcaga cggattcata catgtattcc cggtgaaaaa agacaaaacc ccgcgcaccg
    15781 gaaaggtggc gggggattca cggagataat atctgtctga aattatttag aatgcacctg
    15841 gtgcactgca aaggaaaggc aatattccgt tcctcctcct tgtcgtgaat accccaggta
    15901 tctcacaatg tacaccgact tactccgggg ctgcctgaac cacaagattg ctgtagaatt
    15961 gcccccaatg attcaggtcc aaaccaggaa aatggattaa aaatgacaag tattcaacaa
    16021 cgagcagaac tgcaccgcca gatatggcaa attgccaatg atgtcagagg ttctgttgac
    16081 ggatgggatt ttaagcaata cgttctgggc gcgcttttct accgttttat cagcgaaaat
    16141 ttttccagct atattgaagc cggtgatgac agtatctgtt atgcgaaact ggatgacagc
    16201 gtaattactg atgacattaa agacgatgcc atcaaaacta aaggctactt catctaccca
    16261 agtcagcttt tctgcaacgt agctgcgaaa gcaaatacca atgacagact gaatgcagat
    16321 ttaaacagta tcttcgttgc tatcgaaagt tctgcttacg gttacccttc agaagctgac
    16381 atcaaaggtt tgtttgctga tttcgatacc accagtaacc gcctgggtaa caccgttaag
    16441 gataaaaatg cccgcctggc tgcggttctg aaaggggttg aagggttaaa acttggtgac
    16501 ttcaacgaac atcagattga cctgttcggc gatgcctatg agttcctgat ttctaactat
    16561 gcggcgaatg ctggtaagtc aggcggcgag ttttttacac cgcagcatgt ctctaagctg
    16621 attgcacaac tggctatgca cgggcagacc cacgttaaca aaatctacga cccagccgca
    16681 ggttccggtt cgctgttgtt acaggcgaaa aagcagtttg atgaccatat catcgaagaa
    16741 ggcttttttg gtcaggagat caaccatacg acctataacc tggcgcgtat gaacatgttt
    16801 ttgcacaaca tcaactacga caagtttgat atcaagctgg gcaatacgct gactgagccg
    16861 cacttcagag atgaaaaacc gtttgatgcc atcgtttcta acccgccgta ttcagtgaaa
    16921 tggattggca gcgatgaccc gacgctgatt aacgatgagc gttttgcccc ggctggcgtc
    16981 ctggccccca aatcaaaagc tgacttcgcg tttgtattac atgcgctgaa ctatctttcg
    17041 gccaaaggtc gcgccgcgat tgtctgcttc ccgggtattt tttaccgtgg cggcgcagag
    17101 cagaaaatcc gtcagtatct ggtcgataat aactatgtcg aaaccgtgat ttcactggca
    17161 ccgaacctgt tctttggcac caccattgcc gtaaacattc tggtgctgtc taaacataaa
    17221 acggatacca aagttcagtt tattgacgcc agcgaactgt tcaaaaaaga gactaacaac
    17281 aatatcctga ccgatgccca tatcgaacag attatgcagg tatttgccag caaggaagat
    17341 gttgctcatc tggcgaaatc tgttgcgttt gagactgttg tcgcgaatga ctataacctg
    17401 tcggtgagca gctatgtgga agcgaaagat aaccgcgaaa ttatcaatat tgctgaactg
    17461 aatgcagagc tgaaaaccac ggtcagcaaa atcgaccagt tgcgtaaaga tattgatgca
    17521 attgtggctg aaattgaagg ctgcgaggtg caggtaatga ctccaactta ttgatagtgt
    17581 tttatgttca gataatgccc gatgactttg tcatg
//
LOCUS       DAMZII010000098        17287 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZII010000098 DAMZII010000000
VERSION     DAMZII010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.098"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(387..1184)
                     /locus_tag="POA77_003292"
     CDS             complement(387..1184)
                     /locus_tag="POA77_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6366675.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1194..1745)
                     /gene="ybcL"
                     /locus_tag="POA77_003293"
     CDS             complement(1194..1745)
                     /gene="ybcL"
                     /locus_tag="POA77_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDI6366676.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1914..2246)
                     /gene="emrE"
                     /locus_tag="POA77_003294"
     CDS             complement(1914..2246)
                     /gene="emrE"
                     /locus_tag="POA77_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDI6366677.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLFWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2580..2894)
                     /gene="fliE"
                     /locus_tag="POA77_003295"
     CDS             complement(2580..2894)
                     /gene="fliE"
                     /locus_tag="POA77_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDI6366678.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3109..4767
                     /gene="fliF"
                     /locus_tag="POA77_003296"
     CDS             3109..4767
                     /gene="fliF"
                     /locus_tag="POA77_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI6366679.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4760..5755
                     /gene="fliG"
                     /locus_tag="POA77_003297"
     CDS             4760..5755
                     /gene="fliG"
                     /locus_tag="POA77_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDI6366680.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5748..6434
                     /gene="fliH"
                     /locus_tag="POA77_003298"
     CDS             5748..6434
                     /gene="fliH"
                     /locus_tag="POA77_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDI6366681.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6434..7807
                     /gene="fliI"
                     /locus_tag="POA77_003299"
     CDS             6434..7807
                     /gene="fliI"
                     /locus_tag="POA77_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDI6366682.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPTVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7826..8269
                     /gene="fliJ"
                     /locus_tag="POA77_003300"
     CDS             7826..8269
                     /gene="fliJ"
                     /locus_tag="POA77_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDI6366683.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTHKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8266..9393
                     /gene="fliK"
                     /locus_tag="POA77_003301"
     CDS             8266..9393
                     /gene="fliK"
                     /locus_tag="POA77_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDI6366684.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9498..9962
                     /gene="fliL"
                     /locus_tag="POA77_003302"
     CDS             9498..9962
                     /gene="fliL"
                     /locus_tag="POA77_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDI6366685.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9967..10971
                     /gene="fliM"
                     /locus_tag="POA77_003303"
     CDS             9967..10971
                     /gene="fliM"
                     /locus_tag="POA77_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDI6366686.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10968..11381
                     /gene="fliN"
                     /locus_tag="POA77_003304"
     CDS             10968..11381
                     /gene="fliN"
                     /locus_tag="POA77_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDI6366687.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11384..11749
                     /gene="fliO"
                     /locus_tag="POA77_003305"
     CDS             11384..11749
                     /gene="fliO"
                     /locus_tag="POA77_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDI6366688.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            11749..12486
                     /gene="fliP"
                     /locus_tag="POA77_003306"
     CDS             11749..12486
                     /gene="fliP"
                     /locus_tag="POA77_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDI6366689.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12496..12765
                     /gene="fliQ"
                     /locus_tag="POA77_003307"
     CDS             12496..12765
                     /gene="fliQ"
                     /locus_tag="POA77_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDI6366690.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12774..13559
                     /gene="fliR"
                     /locus_tag="POA77_003308"
     CDS             12774..13559
                     /gene="fliR"
                     /locus_tag="POA77_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDI6366691.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13849..14472
                     /gene="rcsA"
                     /locus_tag="POA77_003309"
     CDS             13849..14472
                     /gene="rcsA"
                     /locus_tag="POA77_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDI6366692.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14516..14704)
                     /gene="dsrB"
                     /locus_tag="POA77_003310"
     CDS             complement(14516..14704)
                     /gene="dsrB"
                     /locus_tag="POA77_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDI6366693.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14867..15094
                     /gene="yodD"
                     /locus_tag="POA77_003311"
     CDS             14867..15094
                     /gene="yodD"
                     /locus_tag="POA77_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDI6366694.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15392..16207
                     /locus_tag="POA77_003312"
     CDS             15392..16207
                     /locus_tag="POA77_003312"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDI6366695.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16204..>17287)
                     /gene="yedQ"
                     /locus_tag="POA77_003313"
     CDS             complement(16204..>17287)
                     /gene="yedQ"
                     /locus_tag="POA77_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDI6366696.1"
                     /translation="QPWFIGHSQRENRHRAVRWFTSQPEHASNTEPQVTVSVPVDSNN
                     YWYGVLGMSIPVRTMQQFLRNAIDKNLDGEYQLYDSKLRFLTSSNPDHPTGNIFDPRE
                     LALLAQAMEHDTRGGIRMDSRYVSWERLDHFDGVLVRVHTLSEGVRGDFGSISIALTL
                     LWALFTTMLLISWYVIRRMVSNMYVLQSSLQWQAWHDTLTRLYNRGALFEKARPLAKL
                     CQTHQHPFSVIQVDLDHFKAINDRFGHQAGDRVLSHAAGLISSSLRAQDVAGRVGGEE
                     FCVILPGASLTEAAEVAERIRLKLNEKEMLIAKSTTIRISASLGVSSSEETGDYDFEQ
                     LQSLADRRLYLAKQAGRNRVCASDNA"
BASE COUNT         4373 a         4394 c         4387 g         4133 t
ORIGIN      
        1 tggcaatttc tgctgtagct gcatcttaac gtataaacta gatttaataa caacgaacta
       61 gatttaataa caacgaacta tatttaataa caacgatatc cgtacctatc cggatatcta
      121 atgtcacaag catcccttta attatcccct caacctcttg taactttccc actcttgaac
      181 tccacatagt ccaaacgccg gatacaaaaa aatcacacgt gcatttctga aattgtgatg
      241 gaggaagata gtatcaatta tatgtgctta taaacgtccc cgattagcgc agaagtcacc
      301 ggattgttca ggctccaata ggttgagtat gccgtattta ttttccaaaa taaaaatcga
      361 tgctgttgct ccttcataaa atacttttac tctctggacg cttttcttag ctctcttggt
      421 gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
      481 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa cgcattattt
      541 aacctcacgt cgtccaatat actttgaaaa cttactccct cgcttgccag acggcgatgc
      601 aatgtggaaa cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcca
      661 ggttcggata gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa
      721 agtaaacaat taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc
      781 tgaatttgcg ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt
      841 aactcgggat tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc ggttatcgca
      901 taaagatttt ggaacttttc aattagatca atgggataat aaagcatttc tgcaagataa
      961 agacctgctt ctgggtaatt cgcaatataa aattcatatc cacagggaaa taatattatc
     1021 tgattattat caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg
     1081 atacgacata aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc
     1141 cgtatttctt caatctttag gttagtccta tcttttgcca gcatactctt atcctacttt
     1201 acctcataaa cgggtattat ctcagcggtt gcaattttat tggcattaag catataacca
     1261 actaacgctc cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatatt
     1321 ttaaactggt aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcccgca
     1381 tagccaaaat catttcggcc ttggacagca ccagtcggaa gttttgtacc atcacgtctt
     1441 cctgcatcag cgggcaaata tgttaccgtt gctggaatat tagcaacagt ccagtgccac
     1501 catccgctgc ctgtaggcgc atctggatca tacacagtta cggcaaagct tttggtgcct
     1561 tctggagcac cagaccaggt taatgagggc gatgtattac ccccctcaca cccaaatcca
     1621 gaaaatacat gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga
     1681 aatgctgcgg cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataagtttt
     1741 ttcataaaaa cctctttgtt gtgacctatc gttattttat ttgatgtgca ttgatctcat
     1801 tatgcaaaaa ggcgcaatga tcgcgcaaat gcaatcataa ttataaagtt gccccgaaca
     1861 ttagccataa tatgcacaaa tcagattcct gttatctcaa ggatgattat tttttaatgc
     1921 ggtgcgcttc gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct
     1981 atgatggctg gcaagtccag tcgttggcca aaaaatcccc agaaaagcaa actaatcaga
     2041 acgataccga ctcctgacca gatagcataa gcaatccctg taggtatata agccagtgtc
     2101 tgagctaata accaaaatga tgcacaatag caaataattg tgccaacaga gggccataac
     2161 cgtgtaaacc cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg
     2221 ccaccaagat aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt
     2281 gtactacagt ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccagggggct
     2341 ctggcgtggc aagagataaa ggcaggaaca ataatgatca gaaggaaccc caagctaaca
     2401 aaagagttca ctcttcctat gagcaaagcg aagacaaaac atgttatcgc gtttacggat
     2461 cgtggatggc agaaaacttt caggatcagg tatccatttt aaaccagaaa ttgagtgagt
     2521 ttgccccatc catgccccaa gcggtgagat tggaagtaat taacaacagg ttacaaaacc
     2581 tacacctgca tggacatcac ttcctgatac gccgccacca gcttattacg cacctgaatc
     2641 cccatttgca tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg
     2701 ccgggttcac cgagggtgaa tttttctgcc tgcgtgcggg cagctgtttg tgtatcgcta
     2761 atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgaa
     2821 tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc
     2881 tgtatcgctg acattttcgt ctcctggata atttctggta gcaaagccta ccagtaagtc
     2941 aataagacaa aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa
     3001 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3061 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3121 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3181 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3241 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3301 agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa
     3361 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3421 ggcggcgcgg tcggtttcga actactcgat caggaaaagt ttggtatcag ccagttcagc
     3481 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3541 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3601 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3661 gaagggcaaa ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg
     3721 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3781 cgcgatctta atgatgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3841 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     3901 gcgcagctgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3961 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4021 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4081 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4141 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4201 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4261 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4321 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4381 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4441 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4501 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4561 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4621 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4681 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4741 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4801 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4861 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga
     4921 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4981 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5041 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5101 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5161 ggtgcatctg aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5221 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5281 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5341 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5401 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5461 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5521 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5581 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5641 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5701 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5761 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5821 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg
     5881 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     5941 agcaaggtca tgagcagggc tatcaggaag gactggcccg ggggctggag caaggtctgg
     6001 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6061 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6121 tggaggcggc acgtcaggtc atcggtcaga cgccaacagt ggataactcg gcactgatca
     6181 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6241 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6301 gctggcgctt gcggggcgat cccacactcc atcctggcgg ttgcaaagtc tccgccgatg
     6361 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6421 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6481 agccaaaatg gcgcagttgc ctacggtacg tcgctacggg cgattaaccc gcgctaccgg
     6541 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6601 ccagaacggc actgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6661 gctgttttta atgccgctgg aggaagtcga aggtgtcctg cccggcgcgc gggtttatgc
     6721 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6781 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6841 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     6901 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgcgggca
     6961 gcgtatgggg ctgtttgccg gatctggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7021 ccgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgtgaagt
     7081 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctcag tagtgattgc
     7141 cgctccggcg gatgtttctc ctctcctgcg aatgcagggt gccgcctatg ccacgcgcat
     7201 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7261 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccactaaagg
     7321 ttatcccccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7381 tagcggcggc ggctcgatta ccgcgtttta taccgtgctc accgagggcg atgaccagca
     7441 ggaccccatt gccgactccg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg
     7501 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7561 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7621 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagtgatcc
     7681 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7741 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7801 gtcataaccc aggagataac ggcagatggc agaacatggc gcgctggcga ccctgaaaga
     7861 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     7921 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7981 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8041 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8101 caaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8161 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8221 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8281 cccttaatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8341 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac taccgacaaa
     8401 gcggcccccc agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc
     8461 agcgagattc ttgccgatgc gcaacaagcg gatttactga tccctgtgga tgaaacaccg
     8521 cctgtcatca acgacgaaca atccacatca acaccattaa ccaccgctca aacgatgacg
     8581 ttggctgcgg tggctggcaa caatacggca aaagacgaaa aagcggatga tctgaatgaa
     8641 gacgtcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8701 aaagtgactg atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8761 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8821 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     8881 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc
     8941 gtcgccgcgc ctgtgttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9001 cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg
     9061 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9121 atggtttcac cgcatcaaca cgtacgcgcc gccctggaag cagcgcttcc ggtactgcga
     9181 acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9241 agtggtcagc agcaggccgc ttcccagcaa cagcaaagcc aacgcacagc aaaccatgaa
     9301 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9361 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9421 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9481 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9541 ggatcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctatt
     9601 ggcattcgca tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg
     9661 tcttctatgc gctggatacc tttacggtca atctgggcga tgcggatcgc gtgctgtata
     9721 tcggcataac cctgcgcctg aaagatgaag ctacacgctc gcggctgagt gagtatttgc
     9781 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9841 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     9901 ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat
     9961 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10021 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10081 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10141 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10201 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10261 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10321 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg
    10381 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10441 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10501 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10561 gtggttaaca caccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10621 ctgccattca gcatgattga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10681 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10741 ctggagttgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10801 aaacccggcg acgtcctgcc gatagaaaaa cccgatcgca tcatcgctca tgttgacggc
    10861 gttccggtac tgaccagtca gtacggcacc ctcaacggtc agtatgcgtt acggatagaa
    10921 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10981 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gctttgagcg
    11041 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11101 gtgatgtcag cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga
    11161 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11221 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11281 taattgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11341 tcattactcc gtctgagcga atgcgccgcc tgagccgtta gtgatgaata accacgctac
    11401 tgtgcaatct tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat
    11461 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgtccc
    11521 taaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11581 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11641 aatcaatctg ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagcccga
    11701 ttttcagtcg gtcatgaaaa acttgcttaa gcgtagcggg agatcctgat gcgtcgtttg
    11761 ttgtctgtcg cccctgtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg
    11821 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    11881 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    11941 acccgcatca ttattgtttt tggtttattg cgtaacgcgc tgggaacacc ctccgcgcca
    12001 cctaaccagg tattgctggg gttggcgctg tttttgacct tttttattat gtcgccggtg
    12061 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12121 gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag
    12181 gcggatttag ggttgtttgc cagactggcg aatactggcc cgttgcaggg acctgaagcc
    12241 gtgccgatgc gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag
    12301 ataggcttca cgattttcat cccttttttg attatcgacc tggtgattgc cagcgtgttg
    12361 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12421 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12481 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12541 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12601 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12661 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12721 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtagcccgt acaatgatgc
    12781 aggtgacaag cgatcaatgg ctttcctggc taagtctgta cttctggccg ttactgcgcg
    12841 tgctggcgct catttctacc gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    12901 aactgggtct ggcaatgatg atcacgttcg ctattgcccc atcattacct gccaacgatg
    12961 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13021 cgcttggttt caccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13081 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13141 ccgttctagc ccgtatcatg gatatgctgg cgttattgct gttcctgaca tttaacggtc
    13201 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13261 cgttgaacag caatgcgttt ctggcactca ccaaagcagg gagtttgatt ttccttaacg
    13321 ggctaatgct ggcgttaccg ctcattactc tgctgctgac actgaatctg gcattaggtt
    13381 tacttaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13441 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13501 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13561 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13621 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13681 ctttacttta agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc
    13741 tgaaaaatcg cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa
    13801 atattcaggt aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt
    13861 attatggatt tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg
    13921 gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat
    13981 tcacagcgcc cttcagtggt gtttattaat gaggactgtt tcatccatga tgcttctaac
    14041 agtcagcaca tcaagcacat cattaatcaa catcccaata cgttatttat cgtttttatg
    14101 gcaattgcca atgttcattt tgatgaatat ctattggtca gaaaaaattt attgataagt
    14161 tctaaatcga ttaaaccgga atctctcgac gatatccttg gcgatattct gaaaaaagag
    14221 acaacgataa cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt
    14281 atgttgcgaa tgtggatggc aggtcaggga accattcaaa tctctgacca aatgaatatc
    14341 aaagccaaga ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat
    14401 aaacaggtta tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc
    14461 aacatgcgct aacacattct gactggtggt ttcccaccag tcaggctgaa ttagattact
    14521 ctgctttctc cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga
    14581 gcgggtagtc ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca
    14641 gtaccacgcc aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct
    14701 tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg
    14761 ccaggcacgc gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac
    14821 ttaacgactg gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa
    14881 agagtacagc gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc
    14941 gatcaatgaa attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag
    15001 cgtcgatgga cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat
    15061 tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct
    15121 gagggggtcg ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg
    15181 aaatctgatg tgttcatcac cttatccgca atttttttcg ctgacaagaa aatattcgcg
    15241 atatgaatga ttaacgctac ttatgaatag aaatgtgacg tgcgtcaccc cttggtggct
    15301 ctggcgtcaa cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc tgttttgaga
    15361 atcatcctgg taaggggaga actgcgcgat catgttttca attcaacaac cactactggt
    15421 ttttagcgat cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggctgc
    15481 cccctggctc agccgtttac gcgaagcgaa tgttcccgtc attctctgta gcagtaaaac
    15541 ttcagcggaa atgctgtact tgcaaaaaac gttggggcta caagggttac cactgattgc
    15601 cgaaaatggc gcagtgatcc agcttgctga acaatggcag gatatagacg gttttccacg
    15661 catcatctca ggtattagcc atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa
    15721 agaacatttt aaattcacga ctttcgatga tgtcgacgat gcaaccatcg ccgaatggac
    15781 gggattaagc cgtagccagg cggcgctgac gcagctgcat gaggcgtcgg taacgctaat
    15841 ctggcgcgac agtgacgagc gtatggcaca atttaccgct cgtctgaacg aactgggctt
    15901 acagtttatg cagggtgcgc gcttctggca cgtcctggat gcctctgccg gaaaagatca
    15961 ggccgccaac tggattatcg cgacctatca acaattatca ggcaaacgcc caaccacact
    16021 tggcctgggc gatgggccaa acgatgcgcc cttactggag gtaatggatt acgcggtgat
    16081 tgtgaaaggg ctaaaccgtg aaggggtgca tctgcatgat gaggatccga cccgcgtctg
    16141 gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc
    16201 tcgttaagcg ttatcgctcg cgcatacccg attacgcccg gcctgtttag cgagataaag
    16261 ccgacggtca gccagtgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct
    16321 actaaccccc aacgaggcac tgatgcgtat cgtcgtactc ttagcaatca acatctcttt
    16381 ttcatttaac ttcaggcgaa tacgttctgc gacttccgca gcctccgtca gactcgcgcc
    16441 tggcagaatc acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa
    16501 ggaactgcta attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa
    16561 gcggtcatta atcgctttaa aatggtcaag atcgacctga atgacagaaa aaggatgttg
    16621 gtgcgtctga cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacgattata
    16681 taaacgggtt aaggtgtcgt gccacgcctg ccactgcaac gagctttgca gaacatacat
    16741 gttgctgacc atccggcgaa tcacatacca ggagatgagt aacatggtgg taaagagcgc
    16801 ccacagcagg gttaatgcaa tgctgatact accgaaatcg ccgcgcacgc cttcgcttag
    16861 cgtatggacg cgcaccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg
    16921 actgtccata cgaatgccgc cccgcgtgtc atgttccatc gcctgcgcca gcaaggccag
    16981 ttcacgagga tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaaaaatct
    17041 cagcttactg tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa
    17101 aaattgctgc atagtacgca caggaatact catccccagc acgccatacc agtagttatt
    17161 actgtctacc ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg
    17221 cgaagtaaac cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgataaacca
    17281 gggttgg
//
LOCUS       DAMZII010000099        17255 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZII010000099 DAMZII010000000
VERSION     DAMZII010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.099"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(81..812)
                     /gene="rlmB"
                     /locus_tag="POA77_003314"
     CDS             complement(81..812)
                     /gene="rlmB"
                     /locus_tag="POA77_003314"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI6366697.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(992..3433)
                     /gene="rnr"
                     /locus_tag="POA77_003315"
     CDS             complement(992..3433)
                     /gene="rnr"
                     /locus_tag="POA77_003315"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI6366698.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3472..3897)
                     /gene="nsrR"
                     /locus_tag="POA77_003316"
     CDS             complement(3472..3897)
                     /gene="nsrR"
                     /locus_tag="POA77_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDI6366699.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4102..5400)
                     /gene="purA"
                     /locus_tag="POA77_003317"
     CDS             complement(4102..5400)
                     /gene="purA"
                     /locus_tag="POA77_003317"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI6366700.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5504..5701)
                     /locus_tag="POA77_003318"
     CDS             complement(5504..5701)
                     /locus_tag="POA77_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDI6366701.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5783..6787)
                     /gene="hflC"
                     /locus_tag="POA77_003319"
     CDS             complement(5783..6787)
                     /gene="hflC"
                     /locus_tag="POA77_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDI6366702.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6790..8049)
                     /gene="hflK"
                     /locus_tag="POA77_003320"
     CDS             complement(6790..8049)
                     /gene="hflK"
                     /locus_tag="POA77_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDI6366703.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8135..9415)
                     /gene="hflX"
                     /locus_tag="POA77_003321"
     CDS             complement(8135..9415)
                     /gene="hflX"
                     /locus_tag="POA77_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI6366704.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9491..9799)
                     /gene="hfq"
                     /locus_tag="POA77_003322"
     CDS             complement(9491..9799)
                     /gene="hfq"
                     /locus_tag="POA77_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDI6366705.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9885..10835)
                     /gene="miaA"
                     /locus_tag="POA77_003323"
     CDS             complement(9885..10835)
                     /gene="miaA"
                     /locus_tag="POA77_003323"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI6366706.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10828..12675)
                     /gene="mutL"
                     /locus_tag="POA77_003324"
     CDS             complement(10828..12675)
                     /gene="mutL"
                     /locus_tag="POA77_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI6366707.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12685..14022)
                     /gene="amiB"
                     /locus_tag="POA77_003325"
     CDS             complement(12685..14022)
                     /gene="amiB"
                     /locus_tag="POA77_003325"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDI6366708.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKQQNGSNYTVVFTINADAPPPPPPPPVVAKRVEKPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14041..14502)
                     /gene="tsaE"
                     /locus_tag="POA77_003326"
     CDS             complement(14041..14502)
                     /gene="tsaE"
                     /locus_tag="POA77_003326"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI6366709.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14474..16021)
                     /gene="nnr"
                     /locus_tag="POA77_003327"
     CDS             complement(14474..16021)
                     /gene="nnr"
                     /locus_tag="POA77_003327"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDI6366710.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16020..17159
                     /gene="queG"
                     /locus_tag="POA77_003328"
     CDS             16020..17159
                     /gene="queG"
                     /locus_tag="POA77_003328"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI6366711.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17142..17195)
                     /locus_tag="POA77_003329"
     CDS             complement(17142..17195)
                     /locus_tag="POA77_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366712.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3898 a         4935 c         4344 g         4078 t
ORIGIN      
        1 ttgcagtacg taattatctt accagctata gacaaaaaaa aaccatccaa atctggatgg
       61 cttttcataa ttcagacgaa ctagctgcgc tgacgaaccg cttcaaataa gcaaattccg
      121 gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct gatcaactca
      181 tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat caccagcgcc
      241 aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc cgtaccgacg
      301 atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt cacccgaatc
      361 agtggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt gagctgtgcg
      421 gagcgatctt tcggcacaat caccgcgtga acgccagcag cgtccgcgct acgcaggcac
      481 gcgccgaggt tgtgcggatc ggttacaccg tcgaggatca gcaggaacgg ttgatcgagc
      541 gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt cacgcgggcg
      601 ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg gtttgccaac
      661 tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg tttatcttca
      721 cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc
      781 actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta cgttttgcct
      841 gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt gcgtgattat
      901 gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt ttgtcagcaa
      961 tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc ttcgccgcac
     1021 gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt ttcgcttttc
     1081 tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag gcgctgtctg
     1141 gctcaaagtt taccttttta ccgacctgac gacgcttgcc gccttttttg cctgcatcgc
     1201 cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt tcactggaga
     1261 tcagactaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga acttccacgc
     1321 gatcgcccag gcgataagtc tggccgctgg attcccccat caggcgttgc cctacctggt
     1381 caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca atgaacaagt
     1441 cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct ttaaagacgt
     1501 tacctacctg gtcgagcatg aagtcacact tcagccagtc agcgacatcg cgcgttgctt
     1561 cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcaacatc tcttccatcg
     1621 aataatggta gccgccggtt tcagtggtgt tgccctgatg cccctgctct ttcgccagca
     1681 gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc gaagtaaagt
     1741 gcgcatagga ctgcaatgcc aggccaaagt gaccacggtt ttcaggatcg taaatcgcct
     1801 gtttcatcga gcgcagcagc atggtttgca gcatttctgc atctggacga tcggcaaccg
     1861 actccagcag ctccgcgtag tcacgcggtt ccggcttatt accgcccggc agttccagcc
     1921 ccagctccgc cagaacagaa cggaaagagg taatcgcttc ggtgctcggc ttgtcgtgaa
     1981 tacggaacag tgccggttct ttggctttct caacaaaacg cgccgccgag atattcgcca
     2041 gaatcatgca ctcttcaatc aacttgtgtg cgtcgttacg ctgagtctgt tcgatacgtt
     2101 caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat gagatcccgc
     2161 cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct tcgagatgct
     2221 taaccagtgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata tgccagactt
     2281 tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg tagccagtca
     2341 ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct acctgcgggt
     2401 tgagcgagca caggccgtta gagagcactt ccggcagcat cgggataacc tgcgaaggga
     2461 agtacaccga cgtaccacgg ttacgcgctt ctctgtccag cggcgttggc ggacgcacat
     2521 agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt tttttctcgc
     2581 agtaaaccgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc agcggtaaat
     2641 cgcgcaaatc gacacggccc gcttttgctt cttccggcac ttcttctttc agcccggcaa
     2701 cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc agagcgatat
     2761 caaccgccat gccggtgccc atattatcgc caaggacttc gacgatttta cccaccgctt
     2821 tggtgcggcg agtcggacgc tgagtcagtt cgactacgac cacaaagccc atccgcgcgc
     2881 ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg tcaggaacca
     2941 caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt tttggcacca
     3001 gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc ggctgagcca
     3061 gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac aaatcatctt
     3121 tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg gtacctttca
     3181 ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc agttgaccat
     3241 cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt
     3301 gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt aaatgttcga
     3361 ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt tcctggaaag
     3421 gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc gtcactccac
     3481 cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc
     3541 cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc gacaggcagg
     3601 tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca gctcgcgcac
     3661 cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc cattttttcc
     3721 acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga ccatatgatt
     3781 acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc cttctggcaa
     3841 tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta actgcacatc
     3901 aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc agccacaagc
     3961 tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg
     4021 gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg gcaggcaaaa
     4081 tatctgccag gcgtaccaga attacgcgtc gaacggatcg cgcagaatca tggtttcagt
     4141 acgatccgga ccggtagaga tgatatcgat cggcacacca gtcagctctt caatacgctt
     4201 gatgtagttc agcgccgcct gcggcagacc gctacgatct ttcacgccga aggtggattc
     4261 agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt cagctgccag
     4321 cggagtggta gtcacttcgc gaccatccgg catacggtaa gccacgcaga gtttaacctc
     4381 tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg agttcagctg
     4441 taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac gccccgtagt
     4501 tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa acagttcggt
     4561 cgggaacgga cctgcaccta cacgagtgga gtaagctttg aggataccca gaacgtaatc
     4621 aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg tgttggaaga
     4681 agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg caccttcaaa
     4741 catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt caaccaccat
     4801 agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt aatcaaccgc
     4861 ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt ctttcagttt
     4921 ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac gtgctacttt
     4981 atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg cgccacgcgc
     5041 tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac atgcttcaga
     5101 cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca tctctttcat
     5161 cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat tctcgcggag
     5221 aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga gagtatggcc
     5281 tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca gaagatcgac
     5341 gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt tgttacccat
     5401 ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca gagttacagc
     5461 actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca atcgttttcc
     5521 tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa
     5581 tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc gggtaaagca
     5641 tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt gtcgaattca
     5701 ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg tggctttatt
     5761 gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga
     5821 agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag ctgttctcat
     5881 aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat gcatcagcaa
     5941 acagtttggc tgcttcggca tcgccttcac cacgcatgat gcggccctga cgctcagctt
     6001 ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct
     6061 gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga
     6121 tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg acaacttcaa
     6181 tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc gtctcagccg
     6241 ttacgcgctc tgccgcttcg gcaatggcgt tatccgctgc cggggtagta acttcatctt
     6301 ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg
     6361 aatcggtgac gatatctttc acgtccaggc gaccaatttc agaacgcaga cggtcagaga
     6421 acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt gccaggtagt
     6481 aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg tctttcttct
     6541 ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg tcgagcattt
     6601 tcaccgtttc aatgaacggt atcttgaaat gcagaccagg ctcataaacc aaaggtttgt
     6661 tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca ccttctttga
     6721 cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg ataactgact
     6781 tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg
     6841 gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg ttgtggagga
     6901 agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc
     6961 gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac ctttatcgtt
     7021 aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat acagacgctc
     7081 gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc gcgccacttc
     7141 accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga ggatacgttg
     7201 cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt cacgaatgta
     7261 ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta cttcttccgg
     7321 cggacgagca gcctggaagt tgacgtccag cagcgtgata cccatgtcat acggacgaat
     7381 cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt ccgtcagaat
     7441 gcggtccatg gtgtatttac cgataactcc acgcagggcg ctgtcggtag cctgacgcag
     7501 gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtga cgcggtactg
     7561 cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac cagaagcggc
     7621 cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg tcggtttcca
     7681 gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca cgccgcgttc
     7741 ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg ctgccgctgc
     7801 gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc
     7861 agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt tgcggaagat
     7921 atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc cctcagagtt
     7981 gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac cgggctgatt
     8041 ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct ttcgacgcca
     8101 gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg atcaacgccg
     8161 gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc agacttacgc
     8221 tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa cgacttctca
     8281 gacgcccttc ctgcggcggc aaacgcaatg tatgctgcgc cacctcgccg gaaagccgct
     8341 ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa agccagacac
     8401 ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc agcatatcga
     8461 tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca agaaccgtat
     8521 tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg tgcagcagta
     8581 atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga tcgtgcggca
     8641 ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc accgacatct gcaacgtcaa
     8701 tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag acccgcgctt
     8761 cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc agcgaaacag
     8821 taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt tcaactcttt
     8881 ccaggcgtga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg gtttcgagct
     8941 gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg tgggtccagc
     9001 cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc aacttaccct
     9061 catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg tcgataacac
     9121 gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca tggtcaaaaa
     9181 gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct ttaccttcac
     9241 ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc aatgcttcga
     9301 caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct ttgtcttgcg
     9361 taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca aacaagcgta
     9421 taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg taaaaaaaca
     9481 gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc
     9541 taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga tgagaaaccg
     9601 gacgagacgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg accgtgtttt
     9661 tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta ataccattca
     9721 ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac ggatcttgta
     9781 aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact ttgaaccttt
     9841 cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc tgcgatagca
     9901 ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc aagccagtga
     9961 accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa
    10021 acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga ccacatctgg
    10081 cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg ggcaaaaagc
    10141 gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg ctcaatgcgt
    10201 tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg atacggtaga
    10261 gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc
    10321 agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc aaccggatct
    10381 acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg ttgctcaatt
    10441 ctggcccgca cttccgggtc tgccgacggt agcggcgaca acccttccag caatgccttg
    10501 aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt gatatcggcc
    10561 atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga cggatcgcga
    10621 atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt cgccgtcccg
    10681 atatccatcc ctttgtaaat aagggcagaa tcaacgctta tcaactctac tggtaaaatt
    10741 ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat caaaaaaatc
    10801 gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta tcgccggatg
    10861 taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg gacataaccg
    10921 ctccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat gttcgctcat
    10981 cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact gcttcgccag
    11041 gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg gtaaaggcac
    11101 ggccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt ccgccagggc
    11161 agactgcgct ttttctaatg ccgatttttc ttcggcagaa acttttagcc gcaacggaat
    11221 cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc aattgtgcct gacgtagcca
    11281 acgttctgcc actggcaagg ataaaagtga aatgttgcca tcgcgctcca gcaacgcaca
    11341 gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg caagtgcagg
    11401 ttcctgcggt tccggcgctt ttggtttttg catcggcgcg ggcgtttgca aaagctggcg
    11461 atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg gggcagccgg
    11521 acgactgcct gatgctggcg caggagagta gcgcggagct accggctcac gagctgccgg
    11581 ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc gggatggcac gcggtgcggg
    11641 ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc tgctgtagca cgctcagcac
    11701 gccctgatag ataaagtcat gcaccagacg cgactgatgg aaacgcactt cgtgtttggc
    11761 ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc agatacaaca caaacgccgg
    11821 ttgttgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcatgat tgatcaggcg
    11881 atcgcgcatc atgcgaccat tcacgtagca atactgaatt tccgccagtg cgggcgtagt
    11941 gtgatttgga tcggccaccc agccgcgcag cgtgagatcg ccgtgttgcc attcaatcgc
    12001 cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct aagcgccgtt ctttttgccc
    12061 gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca ggttgatcgt
    12121 gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca tcaatatggc taaattcggt
    12181 tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat ccagcacctc
    12241 cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc acgtccatgt cgcgtccttc
    12301 ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga
    12361 aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa tggcttcgag
    12421 atcgtccaga gaggcgattt tactggtggc atgacgagcc agcgccagcg ccagctcatc
    12481 ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac cgcgttcgat
    12541 atcaatatcg atacgcgtcg cacctgcatc gaggctgttt tccactagtt ctttgactac
    12601 cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa
    12661 gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt
    12721 actggcagtt tgtgccgttg caccctgcgg cgcagattgc atcggatgcg caaggaagta
    12781 attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt cgctcgccag
    12841 caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg acgggatatc
    12901 cggcgaacgc agaacgccga gactcgcgtg ttctggtcgg cgtttatgta tttcgccaat
    12961 gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgct gggaatgacc
    13021 gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt tcgccagcac
    13081 atcacccgcc ccacccagca gctccgactg tttctcgtgc tgctccagcc agctggccat
    13141 ttcgctgttg gcgcgacggt tagagagcac ccatacggaa gcgccagtcg cactgcggtt
    13201 cggtgcggca tcagcgtgaa tcgacacgag gaaattagcg ttttgcttac gtgccacatc
    13261 gctgcgcccc atcaccgaaa taaagtaatc cccgtcacgg gttaaaacgc ctttaaacat
    13321 tggatcgtca ttgagcaaag tacgcaattt acgtgcgatg gcgatggtga catttttctc
    13381 ccgcgtacca ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc
    13441 gatgataatt ttatcgccag tgttagccgt cgcgcgcgct gccggacgcg ttaccgtatt
    13501 actgctgata acacccgtag tgcggttact ttcggtttta aacggattgc gcgccggttc
    13561 gctgacgcgc ggtgcgacaa ccgcaggttt ttcaacgcgt ttcgcaacca cgggcggcgg
    13621 aggaggcggt ggcggcgcat cggcgttaat cgtaaagacg acagtgtaat tgctgccatt
    13681 ctgctgcttc accgcttcgg ttttaccgtt ttcggtaaga tcgaccacca gccgtagcgt
    13741 ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc agattattgc cgctgaacaa
    13801 caacggcagt ccctgaatca cgcccgtttg tttgatatcg agcgccacgg tgcgtttgct
    13861 ttgatggcta aacgcataat caggatcgcc aataaaactc aacgttatcc gcgcctgttg
    13921 attaccgtta gaaacctgaa tatcagagag cgtcgcggca cccaccggcg tgcacagcag
    13981 cagcagcgtc gctaccaacc aatttctgat gcgatacatc atcccgccac ctttcaaagg
    14041 ttaaccggct aaacgcgcca gcaacaattc acccgcagag gaaaccgcac tcacgcgcgc
    14101 ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca tccgggtcag gaagaacacc
    14161 tgtaccttgt tgtggccact ccaccaggca gatggcatcg ttggcaaaat aatcgcggat
    14221 ccccataaac tccagctcct cgggatcggc aaggcggtac aaatcaaagt gataaaccat
    14281 taaattgtcg agcgtatagg gttcgaccag cgtataagtg gggcttttga cattaccctg
    14341 atgacccaga gcttgtaaaa agccccggct aaaggtggtt ttacctgcgc ctaaatcgcc
    14401 atacagatag attacggttg cgccatcgca ggctttcgct acccgctcgc ccaggtctaa
    14461 tgttgcctgc tcatcaggga gcggaattac tcgattcatc atggttttta tcagtcactt
    14521 ccgggttaac aatacgctgt agcgtggaaa agagatcggt tgccagcatc ccgcgcgttc
    14581 caaaacgcgc cgccagtacg tcagctgcag caccgtgcgc gacacagccc gcacaggcgg
    14641 catcatacgg cgacagtttt tgcccaagca atgcgccaat aataccagag agcacatcgc
    14701 ccatgccgcc gctcgccatg cctgcatttc cggcatcaat aatgcctaaa gcgtcaggat
    14761 gggcggcgac cacggttccg gcacctttca gcaccgctac gccgccataa cgttgtacca
    14821 gacgtttggc gcaatgtaag cggtcacttt caatttcagc gacggaacag cctaacaacc
    14881 gtgcggcctc gccaggatgc ggcgtgatca cgcgattgtg acgcttatcg ggattgattg
    14941 ccagcaggtt caatgcatcg gcatcccaca acatcggttt gcgaaaattc tcaacttttt
    15001 gcagtgcttt tttcccccac tcttgctggc ccagaccggg accaatcacc accacatcgg
    15061 cccattccag gctttcggta agagagtcca tcgtcagttc atgcaccatc aattccggtc
    15121 gtgcagtcag cagcggcgca atgttttcac tgcgggtcag tactcggact aaaccagcac
    15181 cagcacgtag cgccgcttcc cccgtcatac gaatagcccc cgccgtgccg tgatcgccac
    15241 cgataattac cagccgcccg tgatcgcctt tatgcgaagt cgggcgacgc ggttttagcc
    15301 agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt ctcctgacct gccagccaac
    15361 tatccagccc cagtgagtca aaatgcagtt gtccggtaac atcccgcgct tttccagtga
    15421 gcaagcctgg tttcagcgca ataaaagtga tggtgtgatc ggcgttgatc actgcgcctg
    15481 gggtagcgcc ggtttcagcc agcaggccgg aagggatatc aaccgccgca atcggcgcag
    15541 gatgggaatt agcgtggtcg attaactggc taatggattc gcggggcgct tgctgcaagc
    15601 cggtaccgag cagcgcatca acaatcagat ctaccgattc gggccagaca atattcgaag
    15661 catggatctc gcctcccgcg tttaaccatg cttcgcgtgc cagcgcggcc tcttccggca
    15721 acggtttgtc gctctcctgg gccagcagcg tgacctcaat gccgaccgct gtggccagcc
    15781 gcgcgaccac gtagccatcg ccgccgttat taccatgacc gcacagcacc agccaatggc
    15841 gggcgtcagg atacgccgaa cgacacacct ggaatgcggc ttcgccagcg cgaagcatca
    15901 gctcatagag tgtaagcccc agcgcatctg ccgcctcacg ttctccgcgg cggatatcgt
    15961 cggcgtgcca gacggtgtgt ggtatactta cggggttttt cttcattgta tggtccgtca
    16021 tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat taaacagtgg gggctggaac
    16081 tgggctttca gcaggtaggt attaccgata ccgatctcag cgagtccgag cccaaactgc
    16141 aagcatggct ggacaaacaa taccacggcg aaatggactg gatggcacgt cacggtatgc
    16201 tgcgcgctcg cccccatgaa ttattgcccg gtacgctgcg cgtgatcagc gtgcggatga
    16261 attaccttcc tgctaacgcc gcatttgcca gcacgctgaa aaaccccaaa ctcggctatg
    16321 ttagccgtta tgcgctgggc cgtgactatc acaaacttct gcgcaaccga ctcaaaaagc
    16381 tgggcgagat gattcagcaa cattgtgttt cgctgaattt tagaccgttt gtcgattctg
    16441 cgcctattct cgagcgcccg ttagctgaaa aagctgggct cggctggaca ggtaagcact
    16501 cacttatcct caatcgcgag gccggttctt tcttcttttt aggcgaattg ctggtcgata
    16561 ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg taaatgcgtg gcctgtatga
    16621 cgatttgccc gaccggcgcc atcgtcgagc catataccgt cgatgctcgc cgctgtatct
    16681 cttatctcac catcgaactt gaaggggcga tcccggaaga gttgcgaccg ttaatgggaa
    16741 accgtattta cggttgcgat gactgccagc ttatctgccc gtggaatcgc tattcacaac
    16801 tcaccacaga agaggatttc agcccgcgta agccgctaca cgcaccggaa ctcattgagt
    16861 tattcgcctg gagcgaagag aagtttttaa aagtcacgga aggatcggcg attcgtcgta
    16921 ttggtcacct gcgttggctg cgtaatatcg ccgtagcatt aggcaatgcc ccttgggatg
    16981 aaacgatttt gacagcgctg gaaagtcgta aaggtgagca cccacttctc gatgagcaca
    17041 tagcgtgggc gattgcgcag caaatagaga gacgaaatgc gtgcatagtc gaagtgcaat
    17101 tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg gttaccgcgt gacgcctgat
    17161 tcatccacag cttgtgaata aaaataaaaa cgcattgcaa ttcaagacga ataaaatcgt
    17221 caagtgatcg actcgacaaa acggaatctt aattt
//
LOCUS       DAMZII010000100        17234 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZII010000100 DAMZII010000000
VERSION     DAMZII010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.100"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..540
                     /gene="uspC"
                     /locus_tag="POA77_003330"
     CDS             112..540
                     /gene="uspC"
                     /locus_tag="POA77_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDI6366713.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(547..1971)
                     /gene="otsA"
                     /locus_tag="POA77_003331"
     CDS             complement(547..1971)
                     /gene="otsA"
                     /locus_tag="POA77_003331"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDI6366714.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1946..2746)
                     /gene="otsB"
                     /locus_tag="POA77_003332"
     CDS             complement(1946..2746)
                     /gene="otsB"
                     /locus_tag="POA77_003332"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDI6366715.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2913..3899)
                     /gene="araH"
                     /locus_tag="POA77_003333"
     CDS             complement(2913..3899)
                     /gene="araH"
                     /locus_tag="POA77_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDI6366716.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3914..5428)
                     /gene="araG"
                     /locus_tag="POA77_003334"
     CDS             complement(3914..5428)
                     /gene="araG"
                     /locus_tag="POA77_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDI6366717.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5498..6487)
                     /gene="araF"
                     /locus_tag="POA77_003335"
     CDS             complement(5498..6487)
                     /gene="araF"
                     /locus_tag="POA77_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDI6366718.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7284..7787
                     /locus_tag="POA77_003336"
     CDS             7284..7787
                     /locus_tag="POA77_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDI6366719.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7866..8117)
                     /locus_tag="POA77_003337"
     CDS             complement(7866..8117)
                     /locus_tag="POA77_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDI6366720.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8232..8318)
                     /gene="azuC"
                     /locus_tag="POA77_003338"
     CDS             complement(8232..8318)
                     /gene="azuC"
                     /locus_tag="POA77_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDI6366721.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8581..8904
                     /locus_tag="POA77_003339"
     CDS             8581..8904
                     /locus_tag="POA77_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDI6366722.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9075..9572
                     /gene="ftnA"
                     /locus_tag="POA77_003340"
     CDS             9075..9572
                     /gene="ftnA"
                     /locus_tag="POA77_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDI6366723.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9610..9849)
                     /locus_tag="POA77_003341"
     CDS             complement(9610..9849)
                     /locus_tag="POA77_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDI6366724.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10040..11251
                     /gene="tyrP"
                     /locus_tag="POA77_003342"
     CDS             10040..11251
                     /gene="tyrP"
                     /locus_tag="POA77_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDI6366725.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11313..11978)
                     /gene="yecA"
                     /locus_tag="POA77_003343"
     CDS             complement(11313..11978)
                     /gene="yecA"
                     /locus_tag="POA77_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDI6366726.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12174..12260)
                     /locus_tag="POA77_003344"
     tRNA            complement(12174..12260)
                     /locus_tag="POA77_003344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12224..12226),aa:Leu,seq:taa)
     gene            complement(12273..12346)
                     /locus_tag="POA77_003345"
     tRNA            complement(12273..12346)
                     /locus_tag="POA77_003345"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12312..12314),aa:Cys,seq:gca)
     gene            complement(12400..12475)
                     /locus_tag="POA77_003346"
     tRNA            complement(12400..12475)
                     /locus_tag="POA77_003346"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12440..12442),aa:Gly,seq:gcc)
     gene            complement(12627..13175)
                     /gene="pgsA"
                     /locus_tag="POA77_003347"
     CDS             complement(12627..13175)
                     /gene="pgsA"
                     /locus_tag="POA77_003347"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI6366727.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(13232..15064)
                     /gene="uvrC"
                     /locus_tag="POA77_003348"
     CDS             complement(13232..15064)
                     /gene="uvrC"
                     /locus_tag="POA77_003348"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI6366728.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15061..15717)
                     /gene="uvrY"
                     /locus_tag="POA77_003349"
     CDS             complement(15061..15717)
                     /gene="uvrY"
                     /locus_tag="POA77_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDI6366729.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16013..16189
                     /locus_tag="POA77_003350"
     CDS             16013..16189
                     /locus_tag="POA77_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366730.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16176..16400
                     /locus_tag="POA77_003351"
     CDS             16176..16400
                     /locus_tag="POA77_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDI6366731.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16467..17189)
                     /gene="sdiA"
                     /locus_tag="POA77_003352"
     CDS             complement(16467..17189)
                     /gene="sdiA"
                     /locus_tag="POA77_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDI6366732.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         4378 a         4379 c         3975 g         4502 t
ORIGIN      
        1 actaataatc agactaatat ttacctgttt gaccgagttg ggattgcgtc gtttctccat
       61 taggagtaaa gctttaatgt cacctgaagt tcacagaata aggaacaggt tatgagctat
      121 agcaatattc ttgtcgctgt tgcggttaca ccggaaagtc agcaactgct ggcaaaagcg
      181 gtatctatcg ccaggccagt aaagggacac atcagtttaa ttactctcgc ttccgacccg
      241 gaaatgtaca atcaattagc cgcgccgatg cttgaagatt tacgcagcgt gatgcaagaa
      301 gaaacgcaaa gctttcttga taagttaatt caggatgcgg ggtatcccgt tgacaagacg
      361 tttattgcct acggtgagtt aagcgaacat attctggaag tatgtcacaa acatcatttc
      421 gatttggtga tttgcggtaa tcacaatcac agtttctttt cgcgagcatc ctgctcggcg
      481 aaaagagtga ttgcctcaag tgaggtcgat gtgctgttag ttccacttac gggtgattaa
      541 ccgctcctac gcaagctttg gaaaggtagc aactttatcg cgctgctggc tttccgcgct
      601 tcgcggaact atctgcttta ggtcgctaat gaagcactcc tgccagtggt taatatcgtt
      661 tttcacgata acgtccagca tttctgcatg acgggaaata cgttccgcca gcgacatagt
      721 caatgcacga tccagcgcag ctgcaacttc gtcacgatcg taggggttaa caattaacgc
      781 cgacgttaac tcgtttgccg ctcccgcaaa ttgcgaaaga acaagaacgc ccgggttggc
      841 tgggtcctga gcagcaacat actcttttgc taccaggttc atcccgtcac gcagtggcgt
      901 cactaagccc acgtcagagt agcggaatat tttcatcagt aatttacggt caaaatgctg
      961 attcaaataa taaagcggcg tccagcctaa ttgcccgtat ttaccattaa ttcgtccagc
     1021 ttcattttcg agctgatgac gaatatcctg ataggcttgc acatcaccac gcgacgttgg
     1081 tgcaatctgg gtataacgaa ttttaccatg atgctgcgga tatttttcca gcaacgcttc
     1141 ataggcgaga aaacgctctg gcaaaccttt ggaataatcc agccgttcga cagaaaagat
     1201 attttgtacg tttttcagtt ccgctttaag ttgcgccagt tttggcggca gtggcccggc
     1261 agcctgtttg gctatttctt tcggttcaat gccgatcggg tagacttctg ttcgaaatgc
     1321 tttgccccag gctgtatggc tttttgcgct acgtgtcgtg acgcgggtca ggttagaaag
     1381 acaatccagg aacgccagac gatcgttttc tgtctggaaa cccagcaaat cataatcaca
     1441 aagctgttca agcaaggtgt catatgtcgg cagcgcgttg aagatttccg gtgtcgggaa
     1501 aggaatatgc agaaagaaac caatgcgatt attcactccc cgtttgcgta attcatgcgc
     1561 aaatggcaac aggtgataat cgtggatcca gataatgtca tcgtcttgca acagcggcag
     1621 taatttatct gccagcaacg catttacgcg tagatagccg tcccaggcag gacgctgaaa
     1681 ttgcaccaga tcgagccgat aatgaaaagc gggccagaga acggcattgg agaattggtt
     1741 gtagtattcg tcaaggtcct gttcgctgag gttaaaagag gcccacgtaa tgttaccttt
     1801 tttcaccttt tttagcggct gatcctcatt ccctgtttca ccactccagc caaaccacag
     1861 tccgcctgcg gctttcagtg cccccagtat gccaacggca aggccaccgg cactggcggc
     1921 gtgctcgtct ggtggtgcaa tccggttaga tactacgact aaacgactca tagtcatcac
     1981 tcctgttatt ttctcttttt tgttgtaatg cggtggttat catttcaagc cagctccaga
     2041 catccggcac acccgccagt cgccatgatg cctgagttgc acctgtgcca atttttactg
     2101 acattccgcc cagtcggtta acgactgcga agccagattc atcggttaaa tcatcgccca
     2161 gaaatacggg cgttcgcccg ataaagggag cttcctgcat aaaagctgca attgcctcac
     2221 ctttactggt acctctcggt ttgatctcga caacacactt tccctgctgt aacgccattt
     2281 gtggccagat ctgagtaata cgttgcgcta atgtcattaa tgcgtcttca tgctgcggag
     2341 cctgacgata atgcagcgca aaagccatcc ctttcgtctc cagctccgca ccgggatact
     2401 gggcgatgac cgtatgcagt tgcacgctaa tatcacgcgc aatcgcatcc ggcagatgaa
     2461 cgatatgtgt tttaccattg atgtcacggc gctccgcccc atgcacgccc gctaacggga
     2521 agcgataagg ttttgccagt gcgtcaagct ccaccattga gcgccctgat atcaatgcca
     2581 atgcaccatc acttgcggtt gccagtagct gtagtccttg cagaatattg tcaggcacga
     2641 cgacctgatc gggatgcggt ttgatttccg ccagcgttcc atcgagatca aaaaaccagg
     2701 catatttcgc ggatagttca ggggtttcgg ttaacggttc tgtcacccgg ctctcctcct
     2761 tctcttttct tattgttttg cgcagaacgc gctttacaga catgtaagta tagacagtgt
     2821 gacgggggtc gccatttgca atgaagaaca gccaaccgtt gtggcggctg gctgtggtgg
     2881 gaaaaaacgt taaattgttg tggaaaaaag catcagacag tgcgtttcgc tttttgcttg
     2941 taacggtcga agatcaccgc tgccagcagg attaagccgc gaaccacgta ctgcgcgaaa
     3001 ggagaaatat taagcaggtt catggcgttt tccacggtgc ctaaaattaa gatacccgcc
     3061 accacatatg agatttttcc gatgccacct ttcagagaaa cgccacctaa aacgcaggcg
     3121 gagataacaa taagctcata accaatcgac gtcattggct gcccactagt catacgtgaa
     3181 gccagaataa ttccggctat cgcagatacc aggccagaga gaacaaagat aataattttg
     3241 gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt cgttcccgcc aatcgccagg
     3301 gtgttacgac caaaggtggt tttattcagc aacaaaccaa agataatcag acacgcgacg
     3361 gtgagccaga ttggcgcagg cagaccgaac cagttagcgt aaccaagggc aaagaagctt
     3421 tcatcttcga taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc
     3481 tgcatcgttg ccagtgttgt gatcagagca tttattttca gtttggcgat aacaaagcca
     3541 ttgaccaggc cacagagaat gccaagcagc aaccccgctg ccacgccaat ccacaggctt
     3601 tcagtcaggt tgataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca
     3661 gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc cacacgccac catccccgac
     3721 atggaaattg ccaggcccaa ccctttcata ttaatgaagg tggcaaaatt tgggacaaaa
     3781 atggcacagg cgataaagag caccgcaaac accaccagca tgccgtactg atcccagata
     3841 cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg atgtagaaac agaagacatc
     3901 atactctcct tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct
     3961 gacgctcatc tgcctgctcg tgtaacaatt caccggcgat ttcaccttcc cgcatcacca
     4021 caatccggtc ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca
     4081 caccctgcgc cgccagcgca taaatcacgt tgtaaatttc atgcttagca ccaacatcaa
     4141 tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca
     4201 gaatggcttt ttgctgattt ccgcctgaga gattcatgat cagttgctca gcgcccggcg
     4261 ttttgatgtt gagcgaacga atgtggtgat cggcattgtt ttcttcccaa ccgttgttga
     4321 ttacacaacc gccgagcaca tgtttacgtc tggcactgat gttgatattg tcgcgaacgg
     4381 agtgcacggg aataatgcct tccgctttgc gatcttccgg gcagagcatc atgcctgcgg
     4441 caatggcgtg gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg
     4501 cggtgatttg cgtcccgcca aacaagcctt tcattaattc gctacgcccc gctcctacca
     4561 gaccaaacag cccaacaatt tcaccactgc gaaccgccag acttattggc gtacgcacgc
     4621 ctggtgcttt cacagcatca agacgcaggc gctcctcgcc ataactacga ggttgccagc
     4681 cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct
     4741 gctgcatatc ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc
     4801 tgagggcaaa tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc
     4861 gcagttcacg aataacgcgg aaaagattgt cgatttcgcg ggcagaaagg gagctggttg
     4921 gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc cttggcgatt tcaaccatct
     4981 gccactgacc aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat
     5041 gtttaagttg taaacccgcc tcataattca gcaatgagcg attcacaatg ccgcctttat
     5101 gcggcagctg gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagtt
     5161 cctggtaaat aatcgccaca cccgcattaa gtgctgcggt cgtgtcggaa aaggacattt
     5221 cctgcccatt aatcactaca gaacccgtgg ttggcgcata gttgccgctg aggattttta
     5281 agagagttga ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc
     5341 agtcaaaact aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa
     5401 atgagagata cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc
     5461 ccctctgcat catgcagagg ggaatttttc cggcaaatta cttaccgcct aaaccttttt
     5521 tctccagttc ttctttaaag ttgtcacgcg tgatcagtac cacgtcggta acttcggtaa
     5581 attttggcgg ttcaacgtct tttgctaccc agttgtaaag catttcgctg gatttatagc
     5641 catgtacgtc cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gctttagaca
     5701 gttcgctcac cgcatccaca ccgttaatgc caatgccgat gatatcggcc gctttaaagc
     5761 cctgaccttc cgtcgcgcgt acgccgccca gcacggtgct gtcgttcata ccgacgatca
     5821 gccaatgttt aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg
     5881 ggatgtcgtt agatttggta ggtacctgat aaatttgttt ttccgggaat ccggccgctt
     5941 tcagcgcatc catagatccc gtagtacggc ggcgggcggt atccagttcg ttggcggtaa
     6001 tcgccatcac cgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt
     6061 cctggccctg acgttcgcca attttagtcg ccgccatcat caccagcgga acggtatcca
     6121 ttggcttacc tttggcgtta acaaactggt catccacggc aatgactttc atatcgtagc
     6181 cacgcgcttt cgcgacgatg gcagagccga gtttggggtc cggagtacaa ataacgaaac
     6241 cttttgcgcc actggcagcc aggctgtcga tcgcgttcaa tgttttttcg ccatccggca
     6301 cggcaatctt aataacctca aaccctaaat ccttcccggc tttatcggca aacttccatt
     6361 cggtctggaa ccacggctct tccggttgct tcaccagaaa accgagcttc aggttctccg
     6421 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     6481 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     6541 gttaaattaa tcggaaaaca aagcattacc ttttaactaa aagataagtg accgtgttga
     6601 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     6661 gtgagaacgt gcataaattt agcgggaaaa gacataaggg aaagccaatt tgtcagacaa
     6721 attgtcgaat gcacagcaga ttaatccata agattagcct ggaaatcctt gttgtctttg
     6781 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     6841 tttttgaaat ggtgtaataa ccgcaactca aagatgtgga aaatgcacgt cattcatttc
     6901 gtcattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     6961 aaaaatgaaa aaacgtttta taaaatcatc acttcatcat gaattcaaat tcattgatta
     7021 atatcaacaa gatacaaaaa gcactatcat taaaattcat tgcagttaca ttgatttcat
     7081 caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata cccgttcaga
     7141 cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca
     7201 gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac
     7261 ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc
     7321 aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg
     7381 aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc
     7441 aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca
     7501 ttgatgttcc cggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag
     7561 aatacgagca acgttctagt acgttggcac agttagccga tgaagcgaaa gaactgaatg
     7621 atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggtctgt
     7681 tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc
     7741 agaccgacca acatgttctg aatgtcgtgt cacaccagct gcattgatca tcatcggcgc
     7801 taatgcattg cgccgatgaa ggttttgaga aaccgctgcc tcatctgttt gaagcagcgg
     7861 tttttttaat gggattcacc ctgtggggta aacttgagtt caataagcgc gatggctttt
     7921 tggattgccc gcatggtgac cgggtctgcg gcggcgggat ggttagtaaa gtcgatattc
     7981 ttcagctgac tggacatttt ttcacgaact tcaacgggcg cgattacatc gagaacatcc
     8041 agaatttgtt tgataaccaa ctggcaagca accacatcag aaaccagttc ctgatcggca
     8101 ttcagcggct gggacatcgt aaactcctga tagcattttg aaagccgtta tagtagcggc
     8161 ttcacatctt cagcgatagt cacatccacc gtcatcagga cacaaaaaaa cctgccggag
     8221 caggtttttt gttatcggaa catattgcct ggcggtacgt ctttgaacgt cttgcaatag
     8281 ttattgaaca tacttttcag gattttgcgc agtttcatcg cggcactccg accatttgtt
     8341 atacaggtgt tattgtcttt gcgcttataa tatgacaacc atcacaaaaa tcaatcttta
     8401 tgtgatacaa atcacataaa taccccttta atgttataaa aatgataatc aaaaaacagc
     8461 ccccctattt ctgacaccta cagatggcaa gaaatagcgc ctgccaggcg tcttttccgg
     8521 ccattgtcgc agcactgtaa cgcgtaaaat agtgctttct cttactcttc tggctggacc
     8581 atgagacttc tgattctgac tctttcatta ataacgctgg cggggtgtac ggttactcgc
     8641 caagcccatg tgagcgaagt tgacgcggca acaggtattg tacggttagt ttatgaccag
     8701 gcttttttgc agcatgccca taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca
     8761 tgccagcagg aaggctatac ccacgcggtc ccctttggtc agccagtagg caactgtagc
     8821 ctttttgcgg gttccttatg tttgaatacg gaattcactt tgtcatatca gtgccatcat
     8881 tccgccttcc ctgtctttct ctaaatattt taatcagcga ggggatcttc gctgattaaa
     8941 gaaatagcgg taatgcgttt tattcccatt cgtattttta ataattgaag tttatatttt
     9001 accttttgca aataataaaa taacaaatta tagtgacgcc acagcaacaa atataacctt
     9061 tgtggagcac tatcatgctg aaaccagaaa tgattgaaaa acttaatgag cagatgaacc
     9121 tggaactgta ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc tatcatacct
     9181 tcgaaggtgc tgccgcgttc ctgcgccgtc acgcccagga agagatgacg catatgcagc
     9241 gtctgtttga ttacctgact gataccggca atttaccgcg tattaatacc gttgaatctc
     9301 cgtttgctga atattcctca cttgatgaat tattccagga aacctataaa cacgaacaat
     9361 taatcaccca gaaaattaac gaactggctc atgctgcaat gaccaatcag gactacccaa
     9421 catttaattt cctgcaatgg tatgtttctg agcagcatga agaagagaaa ctgttcaaat
     9481 cgattattga taaattaagc ctggcaggca aaagcggcga aggtctgtat tttatcgaca
     9541 aagaactctc taccctcgac acacaaaact aatgctctcc ggcggcaggt ttactgccgc
     9601 cgtttcaaat cagtgacggc aaatcttact ctgatcggtc gaaaaaccgt ctttcgaagg
     9661 tataaatttg ccttttgctg ccagaaacgc taccagctct cccgctgtca tcccttctgc
     9721 cgagcaggtg tgaaaacgtg cctgttcacc aaaacgcgct ttaatcgcag cttccagact
     9781 ggcatgcgta tattgctcgc ctgattcaat catcatattt aacacttcat gaccgtgaat
     9841 agagtccatc gtccctcctc aaaaaaagcc tagcgtagcg attgccgctt atgaagactt
     9901 tgcgccagcg caggactgaa tgctttttat tgtacattta tatttacacc atatgtaacg
     9961 tcggtttgac gaagcagccg ttatgcctta acctgcgccg cagatatcac tcataaagat
    10021 cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga agtgttttta tcgtggcggg
    10081 aaccacaatt ggcgcaggca tgctggcaat gccgctggct gcggccggtg ttggttttag
    10141 cgttacgtta atcttgttga ttgggctttg ggcgttgatg tgctacacgg cgctattact
    10201 gctggaggtg taccagcatg ttccggcaga taccggtctg ggcacgctgg caaaacgcta
    10261 tctgggacgc tacggtcaat ggctgacggg cttcagtatg atgttcttaa tgtatgctct
    10321 gactgcggca tacatcagcg gtgccggtga attgttggcc tccagcatca gcgactggac
    10381 aggtatttca atgtcggcaa ccgctggcgt gctgttgttc acttttgttg ccggtggcgt
    10441 ggtttgtgtc ggaacttcac tggtcgattt atttaaccgt tttctgttca gcgccaagat
    10501 tatttttctg gtggtaatgc tggtactact gctgccgcat attcacaaag tgaatctttt
    10561 aaccctgccg ttgcaacagg ggctggctct gtctgcaatc ccggtgattt ttacctcatt
    10621 tggttttcac ggtagcgtgc cgagtattgt cagctatatg gatggcaacg ttcgtaagct
    10681 acgctgggtg tttataaccg gtagtgcgat ccccctggtg gcatatattt tctggcaggt
    10741 ggcaacgctt ggcagcattg attcaacaac ctttatggga ttgctggcta atcatgccgg
    10801 attaaacggg ctgttacagg cattacgcga aatggtggcc tctccgcatg ttgagctggc
    10861 agtgcattta tttgctgatt tagccctcgc cacgtcattt ctcggcgttg cgttaggctt
    10921 atttgattat ctggctgatt tatttcagcg ttcaaatacc gttggtggac ggttgcaaac
    10981 tggtgcaatt acctttctgc cgccgttggc gtttgcactg ttttatccac gaggatttgt
    11041 gatggcgctg ggttacgccg gtgtggcgct ggcggtactg gcattgatta tcccttcgct
    11101 gttgacctgg caaagcagaa agcacaatcc tcaggcgggt taccgggtga aaggtggtcg
    11161 tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg attggcgtgc aatttttgat
    11221 tgcggcaggg ttgttaccag aagtggggtg atcagatagc ctcaaattcc ttattgggtg
    11281 ccagaattaa cgctgacacc caatttgacc tcttaatgca ggcagcactg cttaaatttc
    11341 ttaccactac cgcacgggca aggatcgtta cgccccggtt tctcttctgc tttgatcggt
    11401 tgctgaacag ctttttcctg cggatgcgcc atccagtacg catgtagatc aagcgccgcc
    11461 agtcgaatgg catctacgct ctcttcaaac gcttctggcg acattttctc tacccgctcg
    11521 aagttttcct cagtaccgtg cagcgcaatc gcctccagcg ctggttttaa cgaatcgggc
    11581 aacgttgacc agtcagaaag tgccacgccc cgcatatagc caaagcacca ctcctcaaca
    11641 atcgtcagct cgctgccatc aacttctcgc aagccgaata acggctcaaa ctgctccggg
    11701 aattcgttca gacgctctgc ggtatcggcc atatgttgaa aagccagatt cataaagcgc
    11761 gtcatctctt tctctgacgc ccagcgcggc acatagtcag ccccacccca cacggcaacc
    11821 agccactgtt ccggttcaat ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc
    11881 agctccgcca catcaaggat ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc
    11941 cattccaact cactttcgtt taacggtccc gttttcatac gcttttcctt gtggatctca
    12001 actcgccagc acctatctta catgccggtc cgtatcagag atactttttg agtggctttg
    12061 ctggtgatta aaaattaagg agggtgtaac gacaagttgc aggcacaaaa aaaccacccg
    12121 aaggtggttt cacgacactg cttattgctt tgattttatt cttatctttc ccatggtacc
    12181 cggagcggga cttgaacccg cacagcgcga acgccgaggg attttaaatc ccttgtgtct
    12241 accgattcca ccatccgggc tcgggaagaa agtggaggcg cgttccggag tcgaaccgga
    12301 ctagacggat ttgcaatccg ctacataacc gctttgttaa cgcgccaaat tcttcaggcc
    12361 tttcagccag acatccgctg acgccgatgt cttttaaact ggagcgggaa acgagactcg
    12421 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
    12481 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
    12541 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatt
    12601 gtttgctgaa aattacgccg aaacgatcac tgttcaagca aatctgcacg cgcagcgctc
    12661 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
    12721 gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac catctgggca
    12781 gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc caactccgcc
    12841 atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc cgccggtaag
    12901 gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc cacgagaact
    12961 ttatctgcga cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
    13021 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
    13081 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
    13141 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
    13201 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
    13261 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
    13321 aaccttgcaa accgcccata tatttcaaca acatttgccg gcgttttggc ccgacgcctt
    13381 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
    13441 tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg ggagaatctg
    13501 gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc ccagccttac
    13561 gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat gagacatcca
    13621 gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg ataaggatca
    13681 catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc acctgattca
    13741 tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac
    13801 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg cccatggtat
    13861 ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
    13921 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
    13981 cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg gtttgaacat
    14041 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
    14101 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
    14161 ggcctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
    14221 agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca caagccatgc
    14281 ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggaa acgaattgtt
    14341 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgcagct tcttcaaact
    14401 ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc tgatcatctt
    14461 tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct tcactcacca
    14521 gtccttcaac gcacggtccc agacagcgcc ctatctggta ttgcagacac ggacgcgagc
    14581 gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc agtagcgcca
    14641 gagtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttggcatgct
    14701 tcgcaccacg atgcatcgcc agacgcgggt gggtatcacc actcaggaag ataaaaggat
    14761 atgatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
    14821 gttccagcaa cagcgcttcg gtttctgtgt gagtaaccgt tacatcaatt tgctggatct
    14881 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag ctggaaagcc
    14941 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
    15001 gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
    15061 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
    15121 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
    15181 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tggtgatcat
    15241 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
    15301 ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac gctgccctga
    15361 atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc
    15421 cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga cggtaagcat
    15481 gatgattttg acatcagctg tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat
    15541 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc accacttaac
    15601 ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat cttccagaat
    15661 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
    15721 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
    15781 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtca
    15841 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca
    15901 gtaagcccta cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
    15961 ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat tcatgataaa
    16021 aatatttatt ggtcattata ttaacgtatt ttatagcact gccgatatca cgctcaaaaa
    16081 acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa catttttcac
    16141 cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat ttttctactg
    16201 ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga
    16261 tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg gaaaaacagc
    16321 ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt aagcaaatta
    16381 aacaagccta ccgtcagtaa tataaaaccg gctgagagcg tgcctttcag ccggtttttg
    16441 catctggcac gcagaaagaa aagcgatcaa attaagccag tagcggccgc gtaacaggcc
    16501 acctgggtct tatttggtgc attgattttt ttctgcatgt ttttctgatg gaaattgacc
    16561 gtattctcag agattgacaa aatcatcgct atctctgctg atgtcttccc ttccgccgtc
    16621 catttcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat cactatttca
    16681 tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat ttttaattgc
    16741 agttcatcac taagaatggg tatttcgcga gcgctgcaac gggaaaagga caaaaagccc
    16801 aacgcccgat ttggcagcat taaatactga gtgacaccgc ggcgtaaacc atgtgcgcgc
    16861 gcggcttccc ataacggctg tgcttcgctg aataagtcat cattccacat taaatggccc
    16921 tgactaaagt tttcagggtt cagcaccgga tcaattgcga gaaaattttt tgcctgataa
    16981 taactaaccc acgactcagg gtaattggtg tagaaagcca ctttaggtcg cgtgaatggt
    17041 accgggtggc gaacacataa cgaatagtaa tcgtactcca gctgctgagc ctgaagctct
    17101 atttcatggt agacctcttc tgcggcctcc atcctctgaa aacgcaacag catcgtgcga
    17161 cgccagctga aaaaatccgt atcctgcata gtaaaccgca acgcccctga gagtgagtat
    17221 catttataat gata
//
LOCUS       DAMZII010000101        17189 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZII010000101 DAMZII010000000
VERSION     DAMZII010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.101"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            155..757
                     /locus_tag="POA77_003353"
     CDS             155..757
                     /locus_tag="POA77_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6366733.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKALDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(796..1221)
                     /gene="nudI"
                     /locus_tag="POA77_003354"
     CDS             complement(796..1221)
                     /gene="nudI"
                     /locus_tag="POA77_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDI6366734.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1500..2042
                     /locus_tag="POA77_003355"
     CDS             1500..2042
                     /locus_tag="POA77_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366735.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2142..3344
                     /locus_tag="POA77_003356"
     CDS             2142..3344
                     /locus_tag="POA77_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDI6366736.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3399..3869
                     /locus_tag="POA77_003357"
     CDS             3399..3869
                     /locus_tag="POA77_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDI6366737.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(3910..4812)
                     /locus_tag="POA77_003358"
     CDS             complement(3910..4812)
                     /locus_tag="POA77_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDI6366738.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5006..6196)
                     /gene="glpC"
                     /locus_tag="POA77_003359"
     CDS             complement(5006..6196)
                     /gene="glpC"
                     /locus_tag="POA77_003359"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDI6366739.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6193..7452)
                     /gene="glpB"
                     /locus_tag="POA77_003360"
     CDS             complement(6193..7452)
                     /gene="glpB"
                     /locus_tag="POA77_003360"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDI6366740.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7442..9070)
                     /gene="glpA"
                     /locus_tag="POA77_003361"
     CDS             complement(7442..9070)
                     /gene="glpA"
                     /locus_tag="POA77_003361"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDI6366741.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9343..10701
                     /gene="glpT"
                     /locus_tag="POA77_003362"
     CDS             9343..10701
                     /gene="glpT"
                     /locus_tag="POA77_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI6366742.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10706..11782
                     /gene="glpQ"
                     /locus_tag="POA77_003363"
     CDS             10706..11782
                     /gene="glpQ"
                     /locus_tag="POA77_003363"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6366743.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11824..12063)
                     /locus_tag="POA77_003364"
                     /pseudo
     CDS             complement(11824..12063)
                     /locus_tag="POA77_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12245..12895
                     /gene="inaA"
                     /locus_tag="POA77_003365"
     CDS             12245..12895
                     /gene="inaA"
                     /locus_tag="POA77_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDI6366744.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12949..13203)
                     /gene="yfaE"
                     /locus_tag="POA77_003366"
     CDS             complement(12949..13203)
                     /gene="yfaE"
                     /locus_tag="POA77_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDI6366745.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13203..14333)
                     /gene="nrdB"
                     /locus_tag="POA77_003367"
     CDS             complement(13203..14333)
                     /gene="nrdB"
                     /locus_tag="POA77_003367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDI6366746.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14422..16707)
                     /gene="nrdA"
                     /locus_tag="POA77_003368"
     CDS             complement(14422..16707)
                     /gene="nrdA"
                     /locus_tag="POA77_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDI6366747.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4057 a         4191 c         4589 g         4352 t
ORIGIN      
        1 ttttcttatt taggctttgc atttggcaaa attttgaggc attttgccga catcgtagga
       61 tttttaatat tacaccaact gctaattatc gccagaaatg tcgctcaatt ttacggtaat
      121 tgtctggttg cgcttgtcta taggtggagt ttacgtgtta gctttttgcc gctcatcatt
      181 gaagtcaaaa aaatatttca tcattttact ggcgctcgct gcaattgccg gactgggtac
      241 tcatgccgcc tggagtagca atggtttgcc acgtatcgac aataaaacat tggccagact
      301 ggcacagcag cacccggttg tcgttttgtt tcgccatgct gaacgttgcg atcgttcaac
      361 caatcaatgc ttgtcagata aaacaggtat tacggttaaa ggtacccagg atgcccgtga
      421 actgggcaac gcttttagcg ctgacatccc tgatttcgat ctttattcca gtaataccgt
      481 ccggaccatt cagtcggcga cctggttttc cgcgggtaaa aagctgacgg tagataaacg
      541 acttcttcag tgcggtaatg agatttatag tgcaattaag gacttacaaa gcaaagcgct
      601 tgataaaaat atcgttattt tcacccataa tcattgcctg acatatattg ctaaaaataa
      661 gcgtgatgcg acatttaaac ctgattatct ggatggttta gtcatgcatg tggaaaaagg
      721 caaagtttat ctggatgggg aattcgttaa ccactaaatt ttcaatctga cagccagtga
      781 tggctgtcat cattgttaaa gaagaccttt caaacgtaac gtttttcggg tggcaacatt
      841 taaatcataa tgcaccagat cttcaggttt tacccacgcg tagtcctgaa actcttcgtt
      901 tattttcact tctcggttgg cagaaacgca gtcaaaaatc aggtaaatca tataaatctc
      961 ttccttgcga ccatctgcat acgtcttggt gcgaatatca tcgctgaagg tccacggcgt
     1021 gatttctgtc aaaagcagct gttctcccag ttcttcgcga atttcgcggc gtagtgcctc
     1081 ttcaattcgt tcgccagact ccacgccgcc acccgaaagc gcccattgac cggggaaaac
     1141 gccgcgatcg tcggccattt tacacagcaa ataagcacca tcattttgaa tcaaagggca
     1201 tacaatagtc cgttgtcgca taggggtatt ccttataaat aaaaagaaac catgagaata
     1261 cggtaactca gaaatatgtg aaagtgacag taatctcttt tgcaatgata actgcactta
     1321 ttattatttt tgtgtgtatc ggtaaagtgt gtttattttt tcggcgttta attatttaag
     1381 aagtatttag ttacaataag tgactagatc ccttttttcg ctcattgaat cctctgtgcc
     1441 aggcagtaca atcccgcagc tttgggaatt ttgattaatt gcttgtggga aaggggataa
     1501 tgaaaaaaat tgcgctagca ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg
     1561 caatgagcat cagcggccag gcgggtaaag aatacaccaa tattggtgtc ggttttggga
     1621 ctgaatcgac gggcctggct ttaagcggta actggacaca taacgacgac gacggtgacg
     1681 tcgcgggcgt ggggctgggg ttgaatctgc ctctcgggcc gttaatggcg accgttggcg
     1741 gaaaaggcgt ttacaccaac ccgaattacg gcgatgaagg ttatgccgca gcggtaggag
     1801 gtggtttgca gtggaaaatt ggcaacagct tccgtttgtt tggcgagtat tactactctc
     1861 cggattcgct ctccagcggt attcaaagtt atgaggaagc gaatgctggc gcgcgttaca
     1921 ccattatgcg tccagtcagt attgaggcgg gttatcgcta cctgaatctg tcgggtaaag
     1981 acggtaaccg cgacaacgcc gtggctgacg gcccgtatgt tggggttaac gccagtttct
     2041 gatcccttcc ggcgcggcat tctgtcgcgc cattcagttt cttactacgc cttatctccc
     2101 tttgcgttat agtgattcga taacaaaagc aggagataac catgttaaaa gtggaaatgc
     2161 tatccaccgg ggatgaagtg ttacacgggc aaatcgttga cactaacgct gcctggctgg
     2221 ccgatttttt ctttcatcag gggctgccat tatctcgccg caatacggtg ggggataacc
     2281 ttgatgactt agtcaccatt cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta
     2341 acggcgggct gggaccgacc agcgatgatt taagcgcact cgccgctgcg acagcaaaag
     2401 gtgaaggcct ggtgctgcat gaagcctggc tcaaagagat ggaacgctat ttccacgaac
     2461 gtggacgagt aatggcaccg agcaaccgta aacaagcgga gctgcctgcc agtgctgaat
     2521 ttatcaataa cccggtaggc accgcctgtg gttttgccgt gcagcttaat cgttgcctga
     2581 tgttctttac tcccggcgta ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc
     2641 cgcgtctgcg cgagcgtttt tctttaccac agccgccggt ttgtctgcgt ttaactacct
     2701 ttggtcgttc ggaaagcgat ctggcacaaa gcctggacac tctacaactg ccgccgggcg
     2761 taacaatggg ctatcgctct tcaatgccga ttatcgaact gaaactcacc ggaccggcaa
     2821 gcgagcaaca ggcgatggaa aaactgtggc tggacgttaa acgtgttgcc ggacagagcg
     2881 tgattttcga aggcaccgaa ggactgcccg cgcagatcag tcgcgaattg caaaaccgcc
     2941 agttcagcct gacgttgagc gagcaattca ccggtggttt attggctttg caactttctc
     3001 gcgcaggtgc tccattgctg gcgtgtgaag tggttccttc acaggaagaa accctggcgc
     3061 aaactgcgca ctggattaca gaacggcggg ccaaccattt tgccgggctg gcactggctg
     3121 tttcgggttt cgagaacgaa catctcaact ttgcgctagc cacaccagac ggcactttcg
     3181 ctctgcgtgt gcgtttcagc actacgcgct acagcctggc tatccgtcag gaagtgtgcg
     3241 caatgatggc actgaatatg ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc
     3301 atggctggat tgaggttatt gagtccatga ccttatctgt ctgattccgc cactctgtgc
     3361 cggtttactg caccggcaca ttccccctcc ggcaaaactt gtccaaccct ccctacggtg
     3421 agcgtggtgt tggggccagt gtggcacggg ctgcgcgctg gggacgcatt gagaattaca
     3481 tggcgcaagt caacgattcg ctttgtctgt tagtgcaggt ggaaagtaaa acggcactgg
     3541 ataacctgga cgagatcctc gacgtcgaag ggattgatgg cgtgtttatt ggaccagcgg
     3601 atctttctgc gtcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta
     3661 ttgaaaccag cattcggcgg atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg
     3721 ctcctgatat ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg
     3781 acacgatgct ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa
     3841 atgggccacg cataaaaggt agttattgat atcaaaggcc catggggatc ggctgtgggc
     3901 ctgtgttaat tagtggttat tcgctgccag atcggcttcg cttagctggg tggccgcgag
     3961 cacctggtca cgatcaatgc cctgttccag catgcgcaaa gcaattttaa tggcttgttc
     4021 atgcatacct tcctgccagc caatttgatg cccttcctgc cgtagccgtt cagcaatagt
     4081 cattaatctc tccttttgta gtggtgaacg ttcggcaatc tcctcaataa aacgggtgaa
     4141 acgggaggta tcgccgcatt gcagcagata attaaacagt gtttgtagct ggctgtcatt
     4201 agtgaagcct ctaaccaaaa gcgtggtgat cctgtcgacc atgccgatta aatcgcggtc
     4261 gcgaatatgc ttttgaatca gttccagcag agctatacgc cgatgttgca tgatctcatc
     4321 gtcaggtacg atggtgatat ccaccaacgg aaaagcttcg gtgtacaact gccgggcaag
     4381 ttgcggatcg tcaaactcat ccagccagtt aggtgagtac gggtagggcg atgtttcgcc
     4441 atgataaaac agcaatggca ccaccagcgg aactctgtca tagcctttat ccaggtgacg
     4501 ctgcatggcg gcagtggcat agcgcattag ccgaaaagcc atattctttt ctgcagagct
     4561 ttgatgttca atcacgcagt agatatagcc gtcaccgtcg ctggtttcca cggaccacaa
     4621 aacatccgag tagtaagcgc gtaaactttt ttcaataaaa ctagtgggtt ccaggcgtaa
     4681 ggtttgcagg ttgcaaagct tgcgcagtgg ttctggtaaa tgtatttcga gaaaatcccg
     4741 tgcggtttcg ggtgtgaaca taaaggtttt aaacaccgca tcatgcgggg aggatgttgt
     4801 tgattcggtc atggttgtcc gtcacctgat atttatgatg acgcgacatt acgtctatcc
     4861 cgggcctttg acatctgtca gtttcagcct ttgcgagaca ttttcagagg aattttcaaa
     4921 gcgttgcaac gcatacatgg tgcgtggaat cgcgctcgca aaaatgcccg ataagcaatg
     4981 tgcttaccgg gcatcaaaag gtcggttaag ccagcgcctg ggccagtagc gtaatcggat
     5041 gttcgcagcg aaggctcgtg gacatctcaa tctgccattt gcaggtttcg cagtcggtga
     5101 tgaccagatc cgcgccgctc tcttctatct ggcggaacag tggcgcaccg atggcttgtg
     5161 aggtggggta gttctctttt ttgaaaccgt aagtacccgc aataccgcag cactgggaat
     5221 ccagcaccgt taactcaagc cccgggattt tacgcaacag ctccagggtg tagagcgtcc
     5281 agcccatttt ttccatatgg cacggagtgt gataaaccac tttcagcggc agcggtttca
     5341 gcggtaacgt tttgccttcg tccagcttgc gccacagcca gcgggttgcc agttcgatat
     5401 gatcgcgcaa gcctttgttg tcgacattca gcacttccgg gtattcgtcg cgcagggcaa
     5461 atgtacaggt tgaggaggtg gcaatcaccg gaatgccttt tactcccaca gcttcgcgga
     5521 tcgactctac attcgtaatt gcctgtttgc gtgctttatc ggtaaagccg ttggcgatta
     5581 gcggtacgcc gcagcatttt tctttgctga gcagttgtac accggtaccc attgcgttga
     5641 gcactttaat taaatcttta cctaactgcg gatggttgta gttaacgaag cagccgtgaa
     5701 agaaagcgac ctggtcttta tattgtgctt gctgagccgc tatgctgcga taccagcgac
     5761 ggaacgtgcc gaaggagtat ttcggtagcg tgcggcgatg atcgattttt aacgccgcat
     5821 caagcagctg ccgcaccggt ttcagcgagg tagcggtgtt gacgattggt gcgaacggcg
     5881 tggaaacgct acccatcagg tcggtatgac tcaacacaaa attacgcagc gacgggcgcg
     5941 tggtgtcata ttttgcccgc gcgcgctgga taatatcgcc aatcttcaca tcggacgggc
     6001 aggcgacttc acaacgtttg cagttgatgc aatatttcag cgcctcgtca tacagtgcgc
     6061 catctttcaa acgcagacgc tcgccatccg gcccggcttg ttttggccct ggataaccgg
     6121 gattcacccg gctcaccggg caggcggtgg tgcagacggt gcacttaatg cagttttcga
     6181 agctggtgtc gttcattgtt ggcctcctgc gcgttgggca atctgttgag cggcatgtaa
     6241 agcactgacg gcacaaacac cgccgccgca tccctgggcg atgggatcaa atccgcccag
     6301 caccgaaccg atggcaaaca ggttctcaat ggtttgccct gcctgcgacg ggcgtagcgt
     6361 ctcatcagtg gttacaccga actgctgcca cggttgcggc gcaaaaaaat cgcccttata
     6421 ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg agaatcggct cgcgaatgcc
     6481 gttacgttcc gctaccagtc cgccactaaa gaaactgccg ctggcgagaa ccgcgaaacg
     6541 tggacgtagc ggaatatcgg cgtgattgcg ggtccagatt tcattcacta cgccattttt
     6601 acaggtcact tttttcactt catcgcccgg catccacacg ccaccctggc gcacaaactg
     6661 gcgctgtaac tggttttgca gacgaatgcc cagcacggaa ggcggcagcg ttggcaaaag
     6721 catcagtgaa caaggtagtt tttcattcaa ccaacgccac agtttgtcat cggccaggcc
     6781 gaagcaggcg ggcatcagga tcatttcgca ggtattggcg acaggaataa gcgcatcaag
     6841 taacagcggc cagttttctt cattatcaag gaaacgggcg atattcacag cgcgaaattc
     6901 ggtggcgtta ttgcgcagga catccagttc cggcagctct atttctgcgg tttcaaccga
     6961 aagatccagt tcgcgcaacg aagccgccgc aagatgcgcc tggaaatcca tcagcccgct
     7021 tatccccact acacatatct tcttcgcggg tagcggccag acgggaactt ctggcgaact
     7081 taaccaggtt gcacgcaaag tgccgagcgg ggtaacgcgc tgatgggcca gttccacgct
     7141 gccctgcaat tgcgcaccgc tttcagcgat caatgcctgc gcctggcaag cgagatcgag
     7201 cacgcgttgt ggttcgagaa gactgtaagg atgggctggt gcctgctgac gcaaagattc
     7261 cagtccactg tgaatgtctg tcaccggttg accatcaggc agatggctca gcaaatccag
     7321 cgaaccggat gagaaatgca gcgcgctttg accacgcgtg acaatggcac agcgcaggcc
     7381 gtgtttttgc agttgcaggc cacagagtaa tccggcgagg ccgccgccca taatgacagt
     7441 atcaaagcgc atctttctgc tccttctcca gaccacacag tccctgataa acccagcggg
     7501 taaattcgct ttcgcgcagt gcgtctcccc aggcgatggg ttgcacgcct ttccagcgtt
     7561 cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg ttaaaacgtt
     7621 gcagcagtcc ggcagcgcgg caggcgcaga gttcgccctg gcaggtaccc atccccacac
     7681 gggtacgacg gcgtaaatcc agcaggctat taacgtttaa attttctacc gcgtactgaa
     7741 cttcacccgc agttaccgct tcgcactcac ataccaggct acgatgcaga cggccttcgc
     7801 tcagccaggc aggcgtgcga tcgccatgac gataaaccgc agaaccgcgc agcggggcag
     7861 gcagggagat gactttacgc aaggtaactt cagcgggttc ttgtgaacca ggcagtgcca
     7921 gatcggcagt cgtacagggg cgcgtgttgc ccagtttgcg gcataccgcg tcggtagccc
     7981 attcagccat cagccgatag gtcatcagtt tgccaccggt gatggtgata aatccgtcca
     8041 gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg gctgacgtta cgtccgctcg
     8101 ggtcgtcatc gctggcaacc agcgggcgca cgccagaata ggcccgcaaa atgcgcgttt
     8161 tcgccatcac gggggccagt ttttcccctt cacgcagcag aatatcaacc tcttctgccg
     8221 tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa agaggtggtg ccaatcagcg
     8281 aaatggtatc gccaggcacc agaatatcgg cgtcggaagg tttacggcag cggttgatca
     8341 catgctggtt aatgcggtga tccatgatca gcagcgatcc tttcgccggg aacatgcgaa
     8401 tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat cccagcggca ttaaccacga
     8461 caggtgcatg aagggcctga gtttcgccgg tgagatggtt acgtacacga acaccgcaca
     8521 ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc ggtaaggata acggcaccgt
     8581 gttctttggc atccagcatg tttgctgcgg tcagacgaaa tggatcaacg gtgccatccg
     8641 gaactttcac cgcgccaatc agtgccgggt taacggcagg ttcgataatg cgcgcttgct
     8701 gcgggtctat agcttctgcg ctgatccctg cttcttcgca ggcgcgaata aaagtggcct
     8761 ggaatgagag gtcatcttcc ggcagggtga taaacaggcc gttggttggt tcaacgcagt
     8821 gacgtgcaat gcgtttcagg atctggtttt cactaatgca ttcgcgggcc gattccgcat
     8881 cggttaccgc atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg gtggcaccgg
     8941 ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc gcgcagggca cagtcgcggg
     9001 caatcccggc tcccgttgcg ccgccaccaa tgataatcac gtcacttgat tgcgagtcgc
     9061 gagttttcat tgttatccct ctgaagttcg ttttttacca tttagccata gtaaaaacat
     9121 gaattgtttg atttcgcgca tattcgctca taattcgaaa gtgaaacgtg atttcatgcg
     9181 tcattctgaa cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt
     9241 ctgaatgttt tcttaacatc gcggcaacta aagaaacggc aggttctccc actgaatcag
     9301 tctgttaatc ataaataaga ccacgggcca cggaggcttt caatgttgag tatttttaaa
     9361 ccagcgccac acaaagcgcg cttacctgct gcggagatcg atccgactta tcgtcgattg
     9421 cgctggcaaa ttttcctggg gatattcttt ggctatgcgg cttactattt ggttcgtaag
     9481 aactttgcgc ttgctatgcc ttatctggtc gagcagggat tctctcgcgg tgatttaggt
     9541 ttcgcgcttt cggggatctc gatcgcttat ggattttcga aattcataat gggttcggta
     9601 tcagatcgct cgaatccgcg cgttttcctc cccgcaggtt tgattctggc ggcggcagtg
     9661 atgttgttta tgggctttgt gccatgggcg acgtcgagca ttgcggtgat gtttgtactt
     9721 ttgttcctct gcggttggtt ccaggggatg gggtggccgc cgtgtggtcg tactatggtg
     9781 cactggtggt cgcagaaaga acgtggcggc attgtgtcag tgtggaactg tgcgcacaac
     9841 gttggtggtg gtattccgcc gctgctgttc ctgctgggga tggcctggtt caatgactgg
     9901 cacgcggcgc tctatatgcc cgctttctgc gccattctgg tggcattatt cgcctttgcg
     9961 atgatgcgcg ataccccgca atcctgtggc ttgccgccga tcgaagagta caaaaatgat
    10021 tatccggacg actataacga aaaagcggaa caggagctga cggcgaagca aatcttcatg
    10081 cagtacgtac tgccgaacaa actgctgtgg tatatcgcca tcgccaacgt gttcgtttat
    10141 ctgctgcgtt acggcatcct cgactggtca ccgacttatc tgaaagaggt taagcatttc
    10201 gcgctggata aatcctcctg ggcctacttc ctttatgaat atgcaggtat tccgggcact
    10261 ctgctgtgcg gctggatgtc ggataaagtc ttccgtggca accgtggggc aaccggcgtt
    10321 ttctttatga cactggtgac catcgcgact atcgtttact ggatgaaccc ggcaggtaac
    10381 ccaaccgtcg atatgatttg tatgattgtt atcggcttcc tgatctacgg tcctgtgatg
    10441 ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag cggcaggtac ggcagcgggc
    10501 tttaccgggc tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat tgttggctac
    10561 accgtggact tcttcggctg ggatggcggc tttatggtaa tgattggcgg cagcattctg
    10621 gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatga acaattactg
    10681 caaaaacgca acggaggcta atggcatgaa attgacgctg aaaaacctta gcatggcgat
    10741 catgatgagc actatagtca tgggaagcag tgcaatggcg gcggacagca acgaaaaaat
    10801 agtcatcgcc catcgcggtg ccagtggata tttgccggag catacgctgc cagcaaaagc
    10861 gatggcgtat gcgcagggag cggattatct ggaacaggat ttggtgatga ccaaagacga
    10921 ccatctggtt gttctgcatg accattatct cgatcgtgtt actgatgttg ccgatcgttt
    10981 cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc tggatgaaat
    11041 taagtcgctg aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag tgcagactta
    11101 tccggggcgt ttcccaatgg gtaagtccga cttccgggtg cacacctttg aagaagagat
    11161 tgaatttgtt caggggttaa atcactctac cgggaaaaat atcggtattt atcctgaaat
    11221 caaagcgccg tggttccatc atcaggaagg gaaggatatt gcggcaaaaa cgctggaagt
    11281 gctgaagaaa tatggttaca ccggtaagga cgacaaagtt tatttgcaat gttttgatgc
    11341 tgatgagctg aagcgtatta agaatgagct ggaacccaaa atgggcatgg atctcaatct
    11401 ggtacagctg attgcctata ccgactggaa tgaaacgcag cagaaacagc cggatggaag
    11461 ctgggttaat tacaactacg actggatgtt taagccgggt gctatgaaac aggtggcgga
    11521 atatgccgac ggtatcgggc cggattacca tatgttgatt gaggaaacat cgcagccggg
    11581 taatatcaaa ctcactggca tggtgcaaga tgctcagcag aataaactgg tagtgcatcc
    11641 ttataccgtg cggtcagata aactgcctga atacactact gatgtgaatc agttatatga
    11701 tgctctgtat aacaaagcgg gtgtaaatgg gctgtttact gatttccctg ataaggcagt
    11761 aaaattcctt aataaagagt aatgctactg caggatggcg ttcatgcgcc atcctgtctc
    11821 acatcaaatt tttccattca ccacgagttg ttccgtacac gtttgtagta aaaacagtgg
    11881 actgccttct accggctttt ctttagccaa caacgaaatc tgtcgataga aggttggttc
    11941 gtgaggaacg gaacacaatt cacctgcaat gcttttcagc gttaactctg gttgtaaggc
    12001 gatgcctaat ccctgtcgaa taaacttcat cgctgtatcc tactgattaa attcacattt
    12061 tatctactgg gcgcactatt agtggtagtg gctggtggaa tctatgcgct gcaaatgaag
    12121 ggtaaaagcg gtgtcacgct agtagtggca aaagaaattc attaatacga cacgtttcat
    12181 taagattttc ctcaggtaac cacctatagt cattgtcgtt aataaaaatg acaggcgata
    12241 gggtatggca gtttcagcaa agtacgacga atttaatcac tggtgggcaa cagagggcga
    12301 ctgggttgaa gagcctaact atcgccgtaa tggtatgagc ggggtgcaat gcgtcgagcg
    12361 caacggcaaa aagctgtatg taaagcgcat gacgcatcat ctgtttcatt ctgtacgtta
    12421 tccgttcggc cgaccaacga ttgtccgtga ggttgcggta attaaagaac tggaacgggc
    12481 cggtgtcatc gtgccgaaga tcgtttttgg tgaagcggtg aaaattgagg gtgaatggcg
    12541 agcgttgctg gtgactgaag atatggcggg gttcatcagc attgctgact ggtatgccca
    12601 gcatgcagta tcgccttatt ctgacgaagt acggcaagcc atgttgaaag cagtggcgct
    12661 ggcgtttaag aaaatgcata gcattaatcg tcagcatggc tgttgttatg ttcgccatat
    12721 ctatgtgaaa acagaaggta aggcagaagc tggttttctt gacctggaga aaagtcgtcg
    12781 ccgcttgcgt cgcgataaag cgattaatca tgacttccgc cagctggaaa aatatctgga
    12841 gccaatcccc aaagcggact gggagcaggt gaaagcgtat tactacgcca tgtaatcgtt
    12901 gtaggccaga taagacgcat aagcgttgta tctggcacta caaccaattc acatctcgat
    12961 ttcaatatcg ccttttgccc ggcaacaaca gggcaaaatt tcccccggct gaataaaggc
    13021 taacggttcg gcaatccagt caacttgacc tgcaaccagg cgtgtgcgac aggagccgca
    13081 gtaaccttcg cgacactgat actcaaccgc cacattgtgg gattccagcg ccgccagaag
    13141 ggaagggtgt tcatcctggc acagcagttg tgtgccagtg atgcgcaggg taacgcgggc
    13201 catcagagtt ggaagttact caaatcgtcg gtgtccactt ccgcgtcaat ctgcccgacc
    13261 agataagaac tgacttccac ttcctgcgga gcaacctgca cgttatcaga caccagccag
    13321 gtgttgatcc atgggatcgg gttggagcgc gtctggaacg gcagatccaa accgactgcc
    13381 tgcatacgga tattggtgat gtattcaacg tactggcaga gaatatcttt attcagacca
    13441 atcatcgaac cgtcacggaa cagataatcc gcccagtctt tctcctgttg agctgcctga
    13501 acaaacaggt catagcactc ctgcttacac tcttcggcaa tttccgccat ctcaggatcg
    13561 tccgcgccgc tgcgcagcag attcagcata tgctgggtgc cggtcaggtg cagggcttcg
    13621 tcgcgggcaa tcaggcgaat aattttggcg ttgccttcca tcaattcgcg ttctgcaaag
    13681 gcaaaggaac aagcaaagct gacgtagaag cggatcgctt ccagcgcgtt aacgctcatc
    13741 aggcaaagat acagtttttt cttcagctcg cgcaggctaa cggttacggt tttaccgtta
    13801 acggtgtggg tgccttcgcc cagaaggtgc cagtagctgg tcatttcgat cagttcatcg
    13861 taatagctgg agatcccttc cgcacgtttc tggatctgct cgttggtgac gatatcgtca
    13921 aacacaacag acggatcgtt aacgatatta cgaatgatat gagtatagga acgggaatga
    13981 atcgtttctg agaacgccca ggtttcgacc caggtttcca gttccggaat agaaataagc
    14041 ggcaatagcg ccacgttcgg actacgaccc tgaatggagt ccagcagcgt ctgatatttc
    14101 aggttgctga taaagatgtg tttttcgtgc tccggcagcg cctggtaatc gatacggtcg
    14161 cgggagacgt caacttcttc cggacgccag aagaaagaga gctgcttttc gatcagcttt
    14221 tcgaagatgt catatttttg ctgatcgtag cgagccacgt tgaccggctg accaaaaaac
    14281 atcggttctt taagctgatc atttttcgtc tgtgaaaagg tggtatatgc catgagtgtg
    14341 tcctgtggtt ttattacccc tcaccctaac cctctcccgc aaggggagag gggacggttt
    14401 ggtgcaaagg ctgggggatt atcagatctt acatgcgccg ctttcgcagc catcgtcctg
    14461 gattgacggc accagatcgt cctgcgcgtc ttcagcgccg tcacgggtgt tctgataata
    14521 cagcgttttg accccgaatt tgtaggcggt gagcaggtct ttcagcaact gctgcatcgg
    14581 cacttttcct gacgggaaac gtgacggatc gtagttggtg ttagcagaga tcgactgatc
    14641 gataaatttc tgcatgatac ctaccagttg caggtagcca tcgttacccg gcatttccca
    14701 cagcagctca taggcgtcgt gcaggtgctc gtagtccggc accacctggc gcaaaatacc
    14761 gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt tcaataccgt tagtggcgtt
    14821 agagatctgc gaagaggtct cggacggcat cagggcagaa agcgtggagt tacgcaggcc
    14881 gtgcgttttg attgattcac gcaatgcttc ccagtcgtaa tgcagcggct cattagcgat
    14941 ggcatccaga tctttcttat aggtatcgat cggcaggatc cctttcgcgt aagtggtttc
    15001 gttaaaccac gggcacgcgc cttgctcttt agccagctca ttagacgctt tcagcaggta
    15061 atactgaatc gcttcgaagg ttttatgcgt caggttgttg gcgctgccgt cggagtagcg
    15121 tttaccgtgc ttcgccagat agtaagcgaa gttaatcaca ccaataccca gcgtacgacg
    15181 ccccatcgct ccacgtttgg cggccgggat cgggtaatcc tgataatcca gcagcgcgtc
    15241 aagtgcacga accgccagaa ttgccagctc ttccagttca tccaggctat taattgcgcc
    15301 caggttgaaa gcagacagcg tacacagcgc gatttcaccg ttctcgtcgt tgacgtcgtt
    15361 cagcggtttg gtcggcaggg ctatctccag gcacaggtta gactgacgca ctggcgcgat
    15421 ggccggatca aacgggctat gggtattgca gtggtcaacg ttctgaatat agatacgacc
    15481 ggtagacgca cgttcctgca tcatcagcga gaacagctca acggctttca cacgctgctt
    15541 gcggatgctg tcgtctttct catatttggt atacaggcgt tcaaactctt cctgatcggc
    15601 gaagaatgcg tcgtacagcc ccggtacgtc ggacgggctg aacagggtga tatcttcacc
    15661 tttcagcagg cgggtataca tcagtttgtt gatctgtacc ccgtagtcca tatggcgcac
    15721 gcggttgcct tccacaccac ggttgttttt caacaccagc aggctttcca cttccagatg
    15781 ccacatcggg tagaacagcg ttgccgcacc gccgcgcacg ccgccctgag agcaggattt
    15841 caccgctgtc tggaaatgtt tgtagaacgg aatacagccg gtatggaacg cttcaccacc
    15901 gcgaatcggg ctacccagcg cacgaatacg cccggcgttg atgccgatcc cggcacgctg
    15961 ggaaacgtat ttcacaatcg cgctggaggt agcgttgatg gaatccagac tgtcaccgca
    16021 ctcgatcagt acgcaggagc tgaactgacg agtcggggta cgcacgccgg acatgattgg
    16081 cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa aaacgcttca catattgcag
    16141 gcgcgtttca cgcgggtagt tcgagaacag gcacgcggca accagaatat aaaggaactg
    16201 ggcgctttcg tagatttcgc cggtcacgcg gttctgtacc agatatttgc cttccagctg
    16261 cttaacggca gcataagaga aggtcatatc acggtcgtga tcgataaacg tgtccatctg
    16321 cttgaactct tcttccgtgt agtcttccag cagatgatta tcgtatttgc ccatctcgac
    16381 cattttcacc acgtggtcgt acagcgcagg cggctcaaac tggccgtagg cttttttacg
    16441 caggtggaag atcgccaggc gcgcggcgag atactgataa tccggcgcat cacgggagat
    16501 caggtctgcg gccgccttga taatggtttc gtggatgtca gaggtcttga taccgtcata
    16561 aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa acgttatgca gtccttctgc
    16621 cgcccaatcc agaacgcgat ggattttgtc gagattgatg cgctctgtgc taccgtcgcg
    16681 ctttgtcacc agcagattct gattcatgta tgtcgtacct gtttttggaa ttctttccgc
    16741 aatagtttca tgcagactac ctgtagtaag ggaagctgct tagaacacta tatatagggg
    16801 gtatgtttgg gattcactgc aagatagtgt gaaaatggcc ctcttgcaag tgcataactt
    16861 tgtggataac tcaggaagga aaaagtggct ttcgcgcacc ttaggtcaga caaggtgtcc
    16921 gggaaagtca atgggaagag aaaaatttgt taaaaataac tgttcggcga tttcttgaac
    16981 ggcagaatag gttggataaa ttgatataga tggtttatta atctttttgc taatgattgc
    17041 aaaaattgat ctaagcacga gtcatcacat aatctgatgt atcagttgtg gtcaatatat
    17101 aaacgaatgt aagcgattgt tgatgtcgaa atggttgttt tctattcggt tattgttaaa
    17161 tgcgaagcca ttttgttaat ttaccttat
//
LOCUS       DAMZII010000102        16997 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZII010000102 DAMZII010000000
VERSION     DAMZII010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.102"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..954)
                     /locus_tag="POA77_003369"
     CDS             complement(<1..954)
                     /locus_tag="POA77_003369"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6366748.1"
                     /translation="MSNITFPIALWEGFEAVHTEQNQQTLIIHLKPIDNGHCACGKPA
                     KAVHDASLRSVSERTILAFQVQLRLPVRRILCPDCGIVREHISWLKPYARQTTRLIEH
                     VEALLKLLPIKHISQLLGLHWHTIKTIDKRRLAREVTEPDWSRVKRLVMDEFALFKGH
                     RYATVVADADTHQVLWIGEGRSREAIRPFFVKLGQYCQQIEAVAMDMNTAFDLEVQKH
                     CPQAKVVYDLFHVVAKYGREVIDRVRVDQANQLKHDKPARKRVKRGRWVLLKNRDNLT
                     DKQAGYLNELLESNKSLMTVYLLREQLKEMWYCTDEAEATAQ"
     gene            1045..2169
                     /locus_tag="POA77_003370"
     CDS             1045..2169
                     /locus_tag="POA77_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6366749.1"
                     /translation="MNLFLGADNVENGTLSLDKTTFISKEKHESMRKGVVQEKDILLV
                     MRGNGTGRSCIYENNAKSYAARINSGLVIIRANSAHTDIQFFQQLVLSPVIQKQFESF
                     MFGSAQPQLTIQLLKSLRFPFPPLPEQKKIAQILSTWDKVIATTEQLLANSQQQKKAL
                     MQQLLTGKKRLLDKNGVRFSGEWKSYHLSHVTVIVMGSSPKSEAYNENGIGLPLLQGN
                     ADIKNRISAPRVFTSEITKECEIGDILLSVRAPVGTVAISEHKACIGRGIAALHAKPN
                     FDQSFIYQFLLWFEPKWGSLSQGSTFESINSDDIKNLKIKMPNFGEQQKIAAVLSTAD
                     QEISALQQKLDALKQEKKALMQQLLTGKRRVTIEKKELLK"
     gene            2169..4100
                     /locus_tag="POA77_003371"
     CDS             2169..4100
                     /locus_tag="POA77_003371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366750.1"
                     /translation="MEIRSPLIHLALLESLKANEISDEIDLFLPFIAVTLSELPGEEV
                     TPAVLQEKFTQSFGITPPISAIQVFMTRAKKRKLLHRENHVFIPNSSQVDKWKNGYHD
                     KKDDIETSLEILRNDFKEFANDHFGKSITDNECDILITEFIDKNISSVTTHNRFEKGQ
                     LKSKIKNTDHVTASFISHIHKTKSSTLDHFSRIVKGMVLANYLCLADKIGSKKKFNSI
                     TAYLDTPIIIGMLGFSGSQRKKSINEFISLLKKVGIELRLFDKSLDEAEMLLGAWRDD
                     LRKRNYRKFNSKTLELLRSQGYDAERLDTEIKLLKSRIEKESITLVSGFRTNPRYQCD
                     EKALESAISTNFKDSKNLEHDTICISRIYNLREGKLIGDLNQKMAIFVTPNTGLVNHA
                     NEYFKNEIPTKKYIPLVVSEQWMTAIFWLKSPEIFNTLPMDQIIASAYGLLYTDDKFW
                     NSFIDKLQLLESKGQITEDDFTVVRWDSDLLGLIHDVSVDVGENFTEKDVFEIVEKIK
                     NKHTQEKEAEITQIKNEKDFEIKELETEIASKDKQIINTQVKIRNISNGIGIATSGLF
                     CLLLMSCVFWTAYTSLPIDMVNYSIIQTYKKSFLTISALFITSVFSFMGTMFGWNVIS
                     VYKWIKNKVSTFVYELLIG"
     gene            complement(4114..5316)
                     /gene="tet(H)"
                     /locus_tag="POA77_003372"
     CDS             complement(4114..5316)
                     /gene="tet(H)"
                     /locus_tag="POA77_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDI6366751.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLHIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            5408..6031
                     /gene="tetR(H)"
                     /locus_tag="POA77_003373"
     CDS             5408..6031
                     /gene="tetR(H)"
                     /locus_tag="POA77_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDI6366752.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(6093..7370)
                     /locus_tag="POA77_003374"
     CDS             complement(6093..7370)
                     /locus_tag="POA77_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6366753.1"
                     /translation="MINSLSLPGYQLVDSDEQNEDIHFRLEAPTPVACEGCCVQGEFV
                     RFGKRDVPYRDLPIHGKRVTLWVVRRRYTCRACKTTFRPQLPEMVDGFRMTLRLHEYV
                     EKEAFNHPYAYVADTTGLDEKTVRDIFNARAEFLGRWHRFETPRILGIDELYLNKRYR
                     CILTNIEERTLLDLLATRRQDVVTNYLMKMKDRQKVKIVSMDMWNPYRAAVKAVLPQA
                     RIVVDKFHVVRMANDALEKVRKGLRKELMPSQSRTLKGDRKILLKRAHEVSNRERLIM
                     ETWTGAFPQLLAAYEHKERFYGIWDATTRPQAEAALDEWIATIPKGQKEVWSDLVRAV
                     GNWREEIMTYFETDMPVTNAYTESINRLAKDKNREGRGYSFEVMRARMLYTTKHKKKA
                     PTAKVSPFYKKTIGYGLPDFAEELNYGVDLSTI"
     gene            complement(7383..8417)
                     /locus_tag="POA77_003375"
     CDS             complement(7383..8417)
                     /locus_tag="POA77_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6366754.1"
                     /translation="MSPQLQDALGMFAFLAIELSVLFIGISLLVGVLQRHIPPSKVEA
                     LLTSSGKRGYFLAAGLGAITPFCSCSTIPMLKGLIQARAGFGPMMVFLFTSPLLNPIV
                     VGLLVATFGWTLTAIYVLAALVVAVGAGWLLHTLGFERYVRRTATQESSGCSSSASPC
                     SATSTASSCGTNSCDTTPKTASCGADRKTTVSTSSECCDSQPTVTVKKEGKYNGVWRE
                     AWSDFIDVLPYLLIAIAIGSVIYGFMPTDLLEQYAGPDNPFAIPVAAVIGVPLYIRAE
                     AVIPLAAALMAKGVGAGTVLALIIGSAGASLTELILLRSLFTLKLLAAFLAVIFAMAM
                     IAGYATYLFF"
     gene            8529..8843
                     /locus_tag="POA77_003376"
     CDS             8529..8843
                     /locus_tag="POA77_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6366755.1"
                     /translation="MDHEKVAASLAELGNSHRLSVFRFLVKAGHDGASVGDIQKGLGI
                     PASTLSHHLARMAKVGLIRQEKHSRTIVCIPEYGHLENLIGFLQEECCAGVRIAHEPE
                     PL"
     gene            9066..12368
                     /locus_tag="POA77_003377"
     CDS             9066..12368
                     /locus_tag="POA77_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6366756.1"
                     /translation="MTQYTPKFQEEYSAKLPALTLLTNLGWSFLSPELALAARGGKAD
                     EVVLRNVLRGELQKRTFIFAGKSYPLSEKAIDNLIAEVCSPALNEGLLTANERLYNHL
                     LYGISVTEFVDGKKASPTVALIDWQNPANNSFVFTEEFTVTRSGGVESRRPDIVCFVN
                     GIPLVVIEAKRPDGNAKKGPTIDEGISQSLRNQRHDEIPLLFAYSQLLLSINGNEGRY
                     GTCGTPAKFWAAWREEDISDAEMYAVKNKTLKDEQLAGLFSHRAAKDLSWYQSLTAAG
                     ELAVTGQDQLLISLLSPARLLEMTRYFTLFDKKAGKIVARYQQVFGIKRLIERINTRS
                     ITGGREGGVIWHTTGSGKSFTMVFLSKALILHESLKQCRIVVVTDRVDLETQLSKTFA
                     SGGELAGKKDKEAAMATSGKRLAEQIGKGTERIIFSLIQKFNTATKMPECVNHSSDII
                     VLIDEGHRSQGGENHIRMKQALPNAAFVAFTGTPLLKDDKTTNKFGPIVHAYTMQRAV
                     EDQTVTPLLYEERIPDLDVNERAIDSWFERITEGLSDEQKADLKRKFAKKGQVYGADD
                     RIRLIALDIANHFIKNIDEGLKGQLACDSKASAIKYKKYLDEAGLFESAVVMSPPDSR
                     EGNTSVDDATTPEVTKWWKDNVGSQDEQAYTKHLIERFDKDDSLKILIVVDKLLTGFD
                     EPKNAVLYIDKPLKEHNLIQAIARVNRLHPKKKFGLLIDYRGILAELDTTIQKYQDLA
                     SRTQGGYDINDIAGLYNQMSTEYKRLPQLYKQLWAIFDGVKNKNDIEQLRQVLVPKIK
                     EQKPLAPGESGELVDVHLKVREDFYEALTTFASCLKVALQSATFFEDKSFSDQDRRHY
                     KETVKQFSSLRQLVQQDAGERIDYDEYAEQVKKLLDKHVVGVEVKEPDGVYEVGKMGQ
                     KQQPEDWSEDKTRNETDIIKTRVTRMIEQELRDDPYAQEAFSKLLRQAIKEAEKLFDH
                     PLKQYMLFREFEEQVQDRRLNDIPDAFAGNRHAQAYFGVFKKVLPEALAVLDQQVQDK
                     WVKVAFEIDQAVETSVAENSINPQNIEADIRKKLLPRMFQECKAIGGGMDQAKKIVEM
                     IVQITRVGLSGA"
     gene            12415..13131
                     /locus_tag="POA77_003378"
     CDS             12415..13131
                     /locus_tag="POA77_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012883149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDI6366757.1"
                     /translation="MSFCYGDEQITFERVKRPQTTGKVLIKVHPDCRVIASAPEGAES
                     EAVLSAVKKRGRWIYEQLRDFRKQLEFTTPRQYISGESHYYLGKQYLLKVIEAPEQVQ
                     GVKLLRGKLEVSVRTKSADKVKELLTDWYKARAKETFAKRLDAMLEQALWVEERPPLR
                     ILTMQTQWGSCSPNGRITLNPHLIKSPRECIDYVILHELCHIAEHNHSERFYRLMSQV
                     MPKWEKTKERLDGMASVIAN"
     gene            13157..13762
                     /locus_tag="POA77_003379"
     CDS             13157..13762
                     /locus_tag="POA77_003379"
                     /inference="COORDINATES: protein motif:HMM:NF037740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366758.1"
                     /translation="MHDYAIFGHSRATIGRWLGVASVILTGAVSSLLVWIYEATNIEA
                     FATAIITPAIIYFVLHYCFDRFGWKIPFFSIPNISGIWSVEGETLNEDGSTKYNWDAE
                     IDIEQTWEKISITLKTAKSSSESYTATLGKKPGTKGGWILHYSYTNNPDTDQFHELNS
                     HKGYCELVFNKDLNQGEAAYFNSNGRRTFGRMTLRKNNDQL"
     gene            13749..14861
                     /locus_tag="POA77_003380"
     CDS             13749..14861
                     /locus_tag="POA77_003380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366759.1"
                     /translation="MINFENRLANLKNRRQGTAERDRLEKGMYSYYGDLRPTEAHEKL
                     HENAAIKYVIGSMAAVSQESTKVSKDEGERVASTLIDMLKTSGINAEAKMQGSVALDI
                     HIEGHSDVDMLILKSDVITVQRPTLPGTYYSDSSDTRPMVDIVRELRLQAEAKLQSRY
                     HAAEVNTSGAKSIALSGGSLRRKVDIVPSSWHDTHDYQGSKLEHFRAVNIYDKDNHAL
                     IENKPFLHIKKVNDRDTQYGGNLKKVTRLMKNIIADMPDYKKSKAKKLTSYDIAAIAY
                     SMNERLSCNQYLPLTLLDNLRSYLLIISYLDDERNALIVPDESRKIFNAEEKVEALRI
                     LYNEVNDLAEAVQKAINPLKEQYDGGVLRNRQVVFF"
     gene            complement(14995..16404)
                     /locus_tag="POA77_003381"
     CDS             complement(14995..16404)
                     /locus_tag="POA77_003381"
                     /inference="COORDINATES: protein
                     motif:HMM:NF018259.1,HMM:NF027308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDI6366760.1"
                     /translation="MKLPVSLPDELLLSRLIRYVTASGYKGDSFASKVFGSRKVSIHP
                     FLTARLEQLAGWGLESADVMLFRQTLAPLFLFFLPTYADKLKQSMLTGDGADAHRASQ
                     LSLFGCGNSLCLKWCPVCAQQDLRQYGVAYWHRAHQIPGVTACAFHPVLLEKQELVRR
                     QRIIAELLPILFDHPRVAFDVEVQVANFGFNLLQLVSGGIPRVDTVLTYRSKLSELGY
                     ITAHHRVRRKSLMEDFCAEIKHYRPGSDTPFPRHPYDHRYLSQLLEPHTSHHPFRHLL
                     FAYWLFREPEKMLFTKHLCAASLQPISLKSLEEEINTEQQCLQLLRKQCSLSEIYRLT
                     GKSRSYLRRLALLNDIPLELKPKKLTGECQKRIIELAYGGMHRKAISERCSIGIGSVE
                     QVISSQPGLVEHRKRCHWESKRRRCRLEIAKYLKLYPRALRRDCKAQCNSAFFWLYLN
                     DPIWLEASLPTAQKPKGRY"
     gene            complement(16425..>16997)
                     /locus_tag="POA77_003382"
     CDS             complement(16425..>16997)
                     /locus_tag="POA77_003382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6366761.1"
                     /translation="NDSVWELFIEELWELQWTNVRTPLTGELNDKLYELSVGNLDLAV
                     RIFREAQRYIIGCGDERITEELLEHAASIAIKASKAVTDEIRRERSITALKRKRNTTS
                     LPARADMAHTQQKELVAVEGVPQSIPGDLSRPHHPEFADALTILQTADDLFERIGNPD
                     LIQHAAAEENPLELLRQEGVFCEHPLKVFA"
BASE COUNT         4919 a         3900 c         4004 g         4174 t
ORIGIN      
        1 ttgagccgtg gcttcggctt catctgtgca gtaccacatc tccttgagtt gctccctgag
       61 caggtaaacc gtcattaagc ttttgttcga ctccaacagc tcgttgagat accccgcctg
      121 tttgtctgtc aaattatctc tgtttttgag taagacccag cgcccccgtt ttacccgttt
      181 gcgggccggt ttgtcatgct tgagttgatt agcctggtca accctgactc ggtcgatgac
      241 ttcacggcca tacttggcaa caacatgaaa caagtcgtag accactttgg cttggggaca
      301 gtgcttctgt acctccaggt caaaggcagt attcatatcc atagcaacgg cttcaatctg
      361 ttggcagtac tgccccaact taacgaaaaa cggccggata gcttctctac tgcggccttc
      421 acctatccaa aggacttgat gagtatccgc atcggccacc acggtggcgt aacgatggcc
      481 tttgaacagg gcaaactcgt ccatgaccag gcgtttgaca cgactccaat caggctctgt
      541 gacctccctt gccagccgac gtttgtcgat agtcttgatg gtatgccagt gcaggccaag
      601 gagttgcgag atatgtttaa tcggcaagag cttgagtagc gcctcgacat gctcaatgag
      661 ccgtgtggtc tggcgagcat agggctttaa ccagctgata tgctcccgaa cgataccgca
      721 atcagggcaa agaatacggc ggacgggtag ccgtaactga acctgaaacg ccagtatggt
      781 tcgttccgat acgctgcgaa gtgaagcgtc gtgaacagcc ttagccggtt tgccgcaagc
      841 acagtggccg ttatcaatcg gctttaggtg gatgatgagg gtttgttgat tttgctcggt
      901 atggacggct tcaaagcctt cccagagcgc aatggggaag gtaatattcg acattgagaa
      961 cggttggggt tggtcattgt ttttgtttgg cgactaaaca ataactctcc aaccgttttc
     1021 atttccagtc cacgctaatc tgcgatgaac ctttttttag gggcagataa tgttgaaaat
     1081 ggtactttat ctttagacaa aaccactttc atatctaaag aaaagcatga atcaatgcgt
     1141 aagggcgttg ttcaagaaaa ggacatactt cttgtaatga gagggaatgg aacgggtcgt
     1201 tcatgcatct atgagaacaa cgcaaagtca tatgcagcac gtataaactc tggtttagtt
     1261 ataatcagag ctaattctgc acacacagat attcagttct tccagcagtt ggtcttgtcg
     1321 cccgtcattc agaaacaatt cgaaagcttt atgtttggga gtgcacagcc ccaattaact
     1381 attcagcttc tgaaaagctt gcgctttcca tttcccccac tccccgaaca aaagaaaatc
     1441 gctcaaatcc tctctacctg ggataaggtc atcgcaacta ccgaacaact gctcgccaac
     1501 agccagcaac aaaaaaaagc cctgatgcaa caactgctca ccggcaaaaa acgcctgctg
     1561 gataagaatg gggttcggtt tagtggggag tggaagtcct accatttgtc tcatgtgact
     1621 gtaattgtga tgggttcgtc acctaaatct gaagcataca acgaaaatgg aattggtctt
     1681 cctttacttc aaggaaatgc agatataaaa aatcgcattt cggccccaag agtttttact
     1741 tctgaaatca caaaagagtg tgaaatcggt gacatattac taagtgttcg agcccctgta
     1801 ggaaccgtcg ctatatctga gcataaagct tgtattgggc gaggaatagc cgcattgcat
     1861 gccaagccta attttgacca aagctttatc tatcaattcc tcctttggtt cgaaccgaag
     1921 tggggctctc tttcccaggg aagcaccttc gagtcaataa atagtgatga cataaaaaat
     1981 ctgaagatta agatgcctaa ttttggcgag caacaaaaaa tcgccgccgt actttccact
     2041 gccgatcagg aaatctctgc tttgcaacaa aagctcgatg ccttaaagca agagaaaaag
     2101 gcactgatgc agcagctgtt aacgggtaaa cgccgcgtaa ctatcgagaa gaaggaactt
     2161 ttaaagtaat ggaaatcaga agtccactta tacaccttgc attgcttgag tcattaaagg
     2221 caaacgaaat atctgatgag atagacctat ttttgccatt tattgctgtt actttatctg
     2281 aacttccagg tgaagaagtt acccccgctg ttttgcagga aaaatttaca cagtcttttg
     2341 gtattacacc gccaatttct gcaatacaag ttttcatgac cagggcaaaa aaaaggaagt
     2401 tacttcacag agaaaaccac gtcttcatcc ctaacagcag tcaagttgat aaatggaaaa
     2461 atggatatca tgacaaaaag gatgatattg aaacatctct tgaaatattg agaaatgact
     2521 tcaaagaatt tgcaaatgac catttcggaa aatcaataac agacaatgag tgtgatattc
     2581 tcataacaga gtttattgat aagaatatat catccgttac cactcacaac aggtttgaga
     2641 aaggccagct caaatcaaag attaaaaata ccgatcatgt tacagcatca ttcataagcc
     2701 atatacataa aaccaaatca tccacactgg atcacttttc aagaatcgtc aaaggcatgg
     2761 ttttagccaa ttatctctgc cttgcagata agattggcag caagaagaaa ttcaactcaa
     2821 ttactgctta tcttgatacg cccataatta ttggcatgtt agggtttagt ggcagccaaa
     2881 gaaagaagtc cattaatgaa tttatttctc ttttaaagaa agtcggaatc gaattgcgtc
     2941 tatttgataa atctcttgat gaggctgaaa tgcttcttgg tgcctggaga gatgacttaa
     3001 gaaaaagaaa ctatagaaaa ttcaattcta agacgctgga attgcttaga tcacaaggat
     3061 acgatgccga gcgattagat accgagataa aacttcttaa atctaggatt gagaaggaaa
     3121 gtataacgtt agtttctgga ttcagaacca atccaaggta tcagtgtgat gaaaaggcat
     3181 tagagtcagc aatatctacc aactttaaag atagcaagaa tcttgagcat gacactattt
     3241 gtatctctag aatctacaac cttagagaag ggaagctcat aggtgatctc aatcaaaaaa
     3301 tggctatttt tgttacgcca aatacgggcc tagtcaatca tgcaaatgag tatttcaaaa
     3361 atgaaatacc gacaaagaaa tatatccctc tggttgtatc tgagcaatgg atgacagcta
     3421 tattctggct taaaagtcca gaaatcttca atacacttcc aatggatcag attattgctt
     3481 cggcttatgg tctcctatac acggacgata agttctggaa ctcattcatt gataaattgc
     3541 aactacttga aagtaaaggg caaattactg aagacgactt tacagttgtt cgatgggatt
     3601 cggatttatt aggtttgata catgatgtct ctgtcgatgt tggtgagaat ttcacagaga
     3661 aagacgtatt tgaaatagta gaaaagataa agaataagca tacccaggag aaagaagccg
     3721 agattacgca aataaaaaat gaaaaagatt tcgaaattaa agagctagag actgaaatag
     3781 cttcaaaaga caagcagatt atcaacaccc aagtaaagat acggaatatt tctaatggta
     3841 tagggatcgc cacatctggg ttgttctgct tattattaat gtcatgtgtc ttttggactg
     3901 cttacactag cctacctatt gatatggtca actattcgat cattcagacg tataagaaaa
     3961 gtttcctaac aatctccgca ttattcatca cgagcgtttt cagctttatg ggcactatgt
     4021 ttgggtggaa cgtaatctcc gtttacaagt ggataaagaa caaagtctcc acattcgtat
     4081 acgagttact tataggctaa ttaatttcta gtattaaggg gttgaaataa cagctttagg
     4141 tgtggttttt ctttggtgaa aataagcggt aataagcaac atagcataaa gtattgcccc
     4201 catcagccat aacagaccat cccaataagc gacgctataa ctataaataa aggcaaataa
     4261 aaggggacca atgatcccgg taatattggt taggctcacc agagtacctt gtaatttccc
     4321 ttgcgcatta tcatcgacag attttgataa ataaccttgt aatgcgggtt gccccatacc
     4381 tcctgccgct aagcaaatta atgctggtaa gatgacccaa acgtggccta tccacgctaa
     4441 taataaacag cccatcatat caatagacat actgatcata atggtggttt tttcgcccca
     4501 tttttgtgcc aatttcccag cgacaatcgc ctgaaagaaa atatgtaata cacccagaac
     4561 cgccaaagac ataccgatag aagttgtgtt ccaatcaaaa cgatattgtg taaacagcac
     4621 ccagatggtg gcaggaattt gcccgataag ctggataata aaataggttg ctaaccaaaa
     4681 gtagaggctt ttcttaaaaa aaacagtgac tgtatttgag gcagtttggt tttcaggtgt
     4741 cctattggca acaagcgctt ctcttttttg tgtttctcgg aaaaagagca aagagagtat
     4801 taataatatc gagtgtgaaa tagcggcaaa aataaatggc atatgagcac tgatatcacc
     4861 taataatccc cctagcattg ggccgataat aaggccaaca ccaaaagcac cacctaagaa
     4921 accaaaatag cgagttcgat ttttagcggg agtcacatca ctcatcgctg atgcacatac
     4981 ggcacctgtt gcgcctgtga tccccgcaat gatgcgccca atatagagca tccaaagtgt
     5041 ggttgagaat gccattaaaa gatagtcgag tgccgcgcct aaaagggaaa acagcaagat
     5101 gggttttctg ccgtatttat cagacagtcg tcctagaata ggagcaaaaa taacctgcat
     5161 ggtagcatag agcgctaata gcacaccgta atgggttgcc agtgaatttt cactgacaaa
     5221 ttcatttaat agagtaggga gtactggcat gataagcccg ataccaatgg catctaatac
     5281 ggtgatcagc agtataataa taattgattt attcattgag atcctaaaaa tctatcagtg
     5341 atagagtggg tgtaaaatat ctctatcagt gatagattgt caaggctatt ttattttgag
     5401 gtaggtaatg gcaaagctag ataaagaaca agttattgat aatgcgttga ttttacttaa
     5461 tgaagttggt attgaaggat taacaacgcg taagctggcg caaaaaatag gtgtggaaca
     5521 acccacattg tattggcatg taaaaaataa acgcgctttg ttagatgcat tagcagaaac
     5581 tattttgcaa aagcaccatc atcatgtttt gccattgccg aatgaaacat ggcaggactt
     5641 tttgcgaaat aacgcgaaaa gcttccgcca agccttatta atgtatcgtg atggtggcaa
     5701 aattcatgcg ggaacacgcc cctctgaaag tcaatttgag acatcagaac agcaactaca
     5761 gtttttgtgt gatgctgggt ttagtctatc tcaagccgtg tatgcattaa gctctattgc
     5821 gcattttaca ttaggctccg tactggaaac tcaagagcat caagaaagcc aaaaagagcg
     5881 tgaaaaagta gagacggata ctgttgccta tccgccatta ttaacccaag ccgttgcaat
     5941 tatggatagt gataatggtg atgctgcatt tttgtttgtc cttgatgtga tgatctctgg
     6001 acttgaaaca gtattaaaga gcgctaaata aatcgggtat acggatttaa tggttgatgg
     6061 ggcaccttta ccccaccaac ctgataccat tatcagatgg ttgatagatc gaccccgtag
     6121 ttgagttcct ctgcgaagtc cggcagtccg taaccgatgg ttttcttgta gaacggagag
     6181 accttcgcgg tcggggcctt cttcttgtgc ttcgtggtgt agagcattcg tgcccgcatc
     6241 acctcgaagg agtaaccacg tccttcacgg ttcttgtcct tggccagtcg gttgatggac
     6301 tccgtgtaag cgttggtgac gggcatgtcc gtctcgaagt aggtcatgat ctcttcgcgc
     6361 cagttgccta cagccctgac cagatcgctc cagacttcct tctggccctt cgggatggtg
     6421 gctatccact cgtccagggc ggcttctgcc tggggccgtg tggtagcgtc ccagatgccg
     6481 tagaaacgtt ccttgtgctc gtaggcggcc agcagttgtg ggaatgcgcc ggtccaggtc
     6541 tccatgatga ggcgttcccg gtttgagact tcgtgagcgc gtttcagcag gattttccgg
     6601 tcacccttga gggttcggct ctgggagggc atcagctcct tcctgaggcc cttgcgcacc
     6661 ttctccaggg catcgttggc catgcgcacc acatggaact tatcgaccac gatgcgggcc
     6721 tgtggcagca ccgccttgac cgctgctcgg tagggattcc acatgtccat gctgacgatc
     6781 ttgaccttct gccggtcttt catcttcatc aggtagttgg tcaccacgtc ctgacggcgg
     6841 gtggccagca ggtcgagcag ggtcctctcc tcgatgttgg tcaggatgca gcggtagcgc
     6901 ttgttcaggt acaactcgtc aatgccgaga atgcggggcg tctcgaagcg gtgccagcgc
     6961 cccaggaatt cggcgcgggc gttgaaaatg tcgcgtaccg tcttctcgtc caggcccgtg
     7021 gtatcagcca cgtaggcgta ggggtgattg aaggcttcct tctccacgta ctcatgcaac
     7081 cgcagcgtca tgcggaagcc gtccaccatc tccggtagct ggggcctgaa ggtggtcttg
     7141 caggcccggc aggtgtaccg gcgacgaact acccagaggg tgacccgctt gccgtggatg
     7201 ggcaggtcac gatagggaac atcacgcttg ccgaaccgca cgaactcacc ctgcacgcag
     7261 cacccctcgc aggctactgg tgtaggtgct tcaagccgaa aatgtatgtc ttcattctgc
     7321 tcatcagaat ccaccaactg gtaaccagga aggcttaacg aattaatcat ctcccgcctt
     7381 gatcagaaaa acaggtaggt ggcgtaaccg gcaatcatcg ccatagcaaa aatgactgct
     7441 aaaaacgccg ctaaaagctt cagcgtgaac aaagagcgca acagaatgag ctcagtcagg
     7501 ctggctccgg cactgccgat gatcaacgca agcaccgtcc cggcacccac gcctttggcc
     7561 atcagagccg ctgccagagg tatgacggct tcagcgcgaa tatacaacgg cacgccgatg
     7621 acagcggcaa ctggaatggc aaagggatta tctgggcctg catactgctc caataagtca
     7681 gtgggcatga aaccatagat cacgctgcca atcgcaatag cgatgagcag gtaaggcaac
     7741 acatcgataa agtccgacca agcttcccgc cacacaccat tgtacttccc ttctttctta
     7801 accgtgacag tgggttgact gtcacagcat tcgctagacg tagagactgt tgtcttccta
     7861 tcagccccgc aagaagccgt cttcggagtg gtgtcgcagc tgttggtgcc gcaactcgat
     7921 gcggtcgatg tagcactgca cgggcttgct gatgaactac atccactgct ctcttgtgtc
     7981 gccgtccggc gcacataacg ctcgaagcca agcgtgtgaa gcagccatcc ggcacctacc
     8041 gcgaccacca aagcggcgag cacatagata gcagtaagtg tccatccaaa cgtggcaacc
     8101 agcaggccga cgacgatagg attcagcaac ggagatgtga aaagaaacac catcatcggc
     8161 ccaaaaccgg cccgtgcctg aatcaaccct ttcaacatgg ggatagtcga gcaactacag
     8221 aagggcgtta tagctcctaa accagcagca agaaaatagc ctcgcttccc actggaagtc
     8281 agtaacgctt ccaccttgga tggtgggatg tgacgttgaa gaactccgac cagcaagcta
     8341 atgccaatga ataaaaccga tagctcgata gcgagaaagg cgaacatgcc tagagcgtct
     8401 tggagttgag gtgacattca atattactcc tatatttcta gttttatcga aatgatgtac
     8461 gcagtctact tgcgtttatc cgacctgtca actataattc tagaaacatc gaattattgg
     8521 ggcgtgctat ggatcacgaa aaggttgcag ccagcttagc tgagctaggg aatagtcacc
     8581 gactgtccgt gttccgcttt ctggtcaaag ctggccatga tggggcttcg gtcggagata
     8641 tccagaaggg gctgggtatt cctgcttcga cgctatcaca tcaccttgcg cgcatggcca
     8701 aggtagggct gatccggcag gagaaacata gccgcacgat tgtctgtata cccgagtatg
     8761 ggcacctaga gaatttgatc ggtttcttac aagaggaatg ttgtgcaggt gtccggattg
     8821 cgcatgaacc agaaccgctt tgacgcttgc ccagctctcg ctgtcctccc tagtcagcat
     8881 cgctatgaag actcaggcga aggtggaaac agaaaattgg cagtagagta ggctgaagag
     8941 gatcaatcag ataatccgct gagaaagatt ttcttcttta catctttgac attttatcaa
     9001 ccagaaattc cggataccca ttaatattcg cacctcgggt atctggggct gaagagagat
     9061 aacgaatgac acaatacaca cccaaatttc aggaagaata cagtgccaag ctacctgcgc
     9121 tgacgctgtt gaccaacctc gggtggtcgt tcctctctcc tgaactcgcg ttagccgctc
     9181 gtggtggcaa ggccgatgag gtggttttac gcaatgtgct acgcggtgag cttcaaaagc
     9241 gcactttcat ctttgccggt aaaagctacc cgctctctga aaaggctatc gacaacctga
     9301 tcgcagaggt gtgcagccct gcacttaacg aaggactgtt aacggccaat gagcgcctgt
     9361 acaaccacct gctgtatggc atctccgtca ctgagtttgt cgatggcaaa aaggccagcc
     9421 cgacggttgc gctaattgac tggcagaacc cagccaataa cagctttgta tttaccgaag
     9481 agtttaccgt tacccgctct ggcggcgtag aaagccgcag acccgacatc gtgtgttttg
     9541 tgaacggtat tcctctggtt gtcattgaag ccaagcgccc ggatggcaat gcaaaaaaag
     9601 gccccaccat tgacgaaggc atttcgcaaa gcctacgtaa ccaacgccat gatgaaatcc
     9661 cattattgtt cgcctatagc caactactgc tttccataaa cggcaacgaa ggccggtatg
     9721 gtacctgtgg cacgcccgca aagttctggg ctgcctggcg tgaagaagac atctccgatg
     9781 ccgagatgta cgccgtcaaa aacaaaacgc tcaaagatga acaattggca ggactattca
     9841 gccaccgcgc ggcaaaagac ttgagttggt accagagcct aaccgctgcg ggggagcttg
     9901 ctgttactgg tcaagatcaa ttactgatca gtctgttaag ccctgctcgg ttactggaga
     9961 tgacccgtta cttcaccttg ttcgataaaa aggctggcaa aatcgttgcc cgttatcagc
    10021 aagtgtttgg catcaagcgg ctgattgaac gtattaatac ccgaagtata accggcggcc
    10081 gtgaaggcgg tgtgatctgg cacaccactg ggtcgggtaa gtcattcact atggtgtttt
    10141 taagtaaggc actgatactg cacgaaagcc tgaagcaatg ccgtattgtg gtggtgactg
    10201 accgggtaga cttggaaacc cagttaagca aaacctttgc ctctggcggc gagctagccg
    10261 gtaaaaaaga caaagaagcg gcgatggcca cctctggcaa acgcttggcc gaacagatcg
    10321 gcaaaggtac tgagcggatc atcttctcgc tcatccagaa gtttaatacc gctacaaaaa
    10381 tgccggagtg tgtaaaccac agctctgaca tcattgtgct gatcgacgaa ggccatcgca
    10441 gccagggcgg tgaaaaccat attcgtatga agcaggcgct acctaatgct gcctttgtcg
    10501 cctttaccgg cacgccactg ttaaaagacg acaagaccac taataagttt ggccccattg
    10561 tgcacgctta caccatgcag cgtgccgtgg aagaccagac tgttacaccg ctgctatacg
    10621 aagagcgcat tcccgacttg gatgtgaatg aacgcgccat cgacagttgg tttgagcgta
    10681 ttaccgaagg gctttcagac gagcaaaagg ccgacctgaa gcgcaagttt gccaaaaagg
    10741 gccaagtata cggtgcggat gatcgcattc gtttgattgc gctggatatc gccaaccact
    10801 tcataaaaaa catcgacgaa ggtttgaaag gccagttagc ctgcgacagt aaggcctctg
    10861 cgatcaagta caaaaagtat ctggacgaag caggattgtt tgaatctgcg gtggtgatga
    10921 gcccgccgga tagccgtgaa ggtaatacct ctgtcgatga tgccactaca cccgaagtga
    10981 ccaaatggtg gaaagacaac gtgggcagcc aggatgaaca agcttacacc aaacacctga
    11041 tagaacgctt cgacaaagat gattcactga agatactcat tgtggttgat aagctgctca
    11101 cgggctttga tgagccgaaa aacgcagtct tgtatatcga caaaccgctg aaagagcaca
    11161 acctgatcca agcaattgca cgggtaaacc gcctgcaccc gaagaagaag tttggtctgc
    11221 tcatcgacta tcgcggcatt ctggcggaac tggataccac catccagaaa tatcaggatc
    11281 tggcctcccg cactcagggt ggttacgaca tcaatgatat tgcaggcctc tacaaccaga
    11341 tgagtaccga gtacaagcgc ttgccacagc tgtataagca gctatgggcc atattcgatg
    11401 gcgtgaagaa taagaacgac atcgagcaat tgcgtcaggt gctggtacca aagattaaag
    11461 agcaaaaacc tttagcgccg ggagagagcg gcgagctggt tgacgtgcat ctaaaggtgc
    11521 gtgaagactt ctatgaggca ctgacgacct tcgccagctg cttgaaggta gcactgcaat
    11581 cggccacctt ctttgaagac aagagcttta gcgaccagga tcgtcgtcac tacaaagaaa
    11641 cggtgaagca attctccagc ctgcgccaac ttgtgcagca agatgccggt gagaggattg
    11701 actacgacga atacgccgaa caagtgaaaa agctgctgga caagcatgtg gtgggtgtcg
    11761 aggttaaaga acccgacggc gtgtatgaag tgggaaaaat ggggcaaaag caacagccag
    11821 aagactggag cgaagataaa acccgcaacg aaacagacat catcaaaacc cgcgtaaccc
    11881 gaatgattga acaagagctt cgggatgacc cctatgctca agaggcattt tccaagctgc
    11941 tgcgccaagc cattaaagaa gccgaaaaac tattcgacca tccactgaaa cagtacatgc
    12001 tgttccgtga atttgaagag caagttcaag acagacgctt gaatgacata cccgatgcgt
    12061 ttgccggtaa tcgccacgct caggcttact ttggtgtgtt caagaaagtg ctgcctgaag
    12121 ctttggctgt actcgaccag caagttcagg ataagtgggt aaaagtcgcc tttgaaattg
    12181 atcaggcggt agaaacctct gtcgcggaaa actccatcaa cccacagaac atcgaagccg
    12241 acattcgtaa aaagctacta ccccgcatgt tccaggaatg caaagccatt ggtgggggca
    12301 tggatcaggc caagaagatt gttgagatga ttgttcagat cactcgggtc gggttgagtg
    12361 gtgcttgaag ggtataacca gatgaatgtg ccgattaaac ctcaaagtca gaccatgagc
    12421 ttttgctacg gcgacgagca gattacgttt gagcgagtca agcgtccgca aaccacaggc
    12481 aaggtattaa tcaaggttca tcccgattgt cgggtaatcg catcggctcc tgagggggct
    12541 gagagcgaag ccgtgttatc tgctgttaaa aagcgtggcc gttggatata cgaacagctt
    12601 cgggatttcc gcaaacagct tgaattcacc acccctcggc aatacatcag tggtgagagc
    12661 cattactacc ttgggaaaca gtacctgctg aaagtgattg aagcgcctga gcaggttcaa
    12721 ggcgtgaaac tgctacgtgg caagttagaa gtttcggtaa gaacaaagtc cgctgacaag
    12781 gttaaagagc tactaacaga ctggtacaaa gcgcgggcca aggaaacctt cgccaagcgc
    12841 ctcgatgcca tgctggagca agccctttgg gtagaagaac ggccaccact tcgcatactg
    12901 accatgcaaa cccaatgggg cagctgctca cccaatggcc gaatcaccct gaaccctcac
    12961 ctgattaaat cgcctcgtga gtgcattgat tatgtgattt tgcacgagct ttgccacata
    13021 gctgaacata accacagcga gcggttctac cggctgatga gccaggtaat gccaaagtgg
    13081 gaaaagacga aagaacggct ggatgggatg gcttctgtta tcgcaaattg attactgaat
    13141 tcataggagc aaacaaatgc acgactacgc aatatttggg cacagcagag ccactatagg
    13201 gagatggctc ggtgttgcat ctgtaatttt aacaggtgct gtttcttcgt tattagtttg
    13261 gatatacgaa gcgacaaaca tagaagcatt cgcaactgcc ataataactc cagccataat
    13321 ttattttgtc cttcactact gttttgatcg gtttggctgg aaaattccgt tctttagcat
    13381 cccgaatatc agtggcatat ggtcagtgga aggtgaaacg ctaaatgaag acggttccac
    13441 aaagtacaac tgggacgccg aaatagatat cgaacaaact tgggaaaaaa tctctataac
    13501 cttaaaaacg gcaaagagtt caagcgaaag ctataccgct acacttggca aaaagccagg
    13561 aacaaaaggt ggttggattc ttcactatag ctatacgaac aaccctgata ccgatcaatt
    13621 tcatgaattg aattctcaca aaggctattg cgaattagtt tttaacaaag atttaaacca
    13681 aggtgaagcc gcttacttca atagtaacgg gagaaggact ttcggaagaa tgactttgag
    13741 gaaaaataat gatcaacttt gaaaacagac tagcaaacct aaaaaaccgc cgccaaggaa
    13801 cagccgaaag agatcgccta gaaaaaggca tgtattccta ctatggcgat ctaagaccga
    13861 cagaagccca tgaaaagcta catgaaaatg ctgcaataaa atatgtaatt ggttcaatgg
    13921 cggcagtcag ccaagagtct acaaaagttt cgaaagatga gggggagcga gtagcatcta
    13981 cactgattga catgctcaaa acgtcaggaa tcaatgccga agctaaaatg caaggctctg
    14041 ttgcattgga tattcatatc gaaggtcatt ctgacgtaga tatgttgata ctgaaatcgg
    14101 acgtaattac tgtccaaaga ccaactttgc ccggtaccta ctactcagat tcaagcgata
    14161 ctcggccaat ggttgatatt gtaagagagc tgagacttca ggcggaagcg aaactccagt
    14221 ctcgctatca tgccgctgaa gtaaacacta gtggggccaa gtcgattgct cttagtgggg
    14281 gtagccttag acgaaaagtg gatatcgttc caagtagttg gcacgatacg catgactacc
    14341 aaggatcgaa gcttgagcac ttcagagctg taaatattta tgacaaagat aatcatgctt
    14401 taatcgagaa caagcctttt ctgcacatca agaaggtaaa tgacagagac actcagtacg
    14461 gcggcaacct taaaaaagta actcggttaa tgaaaaacat cattgccgac atgccagatt
    14521 ataagaaatc aaaagcaaaa aaacttacta gttacgacat tgctgcgatt gcctattcta
    14581 tgaacgaaag gttatcttgc aaccaatatt taccgttaac gctactcgat aacttgcgtt
    14641 catacttgct aatcatttca tatcttgatg atgaaagaaa cgcgcttata gtccctgatg
    14701 agtcaagaaa gatattcaac gctgaagaaa aagtagaggc actaaggata ctctacaacg
    14761 aagtaaatga ccttgctgaa gctgttcaaa aggccattaa ccctttgaaa gaacaatatg
    14821 atggtggtgt attacgaaat cgacaagtgg tgttttttta aagaacaaat gcccctacta
    14881 gttcggcatg cctctctgta tggagttaag gctatgccac taaccatata aattataaag
    14941 ctttttctga aaacactaag tcactttagg tcattaactc tgctaaatac ggctttaata
    15001 tctccccttg ggtttttgag ctgtaggtaa ggaagcctct aaccaaatag gatcattgag
    15061 atacagccaa aagaaagccg aattacactg agctttgcaa tccctgcgaa gcgctctagg
    15121 atacaatttt agatatttgg caatctcaag ccgacaacgt cgacgcttgg attcccaatg
    15181 acaccgctta cggtgttcga caagtcccgg ctggctagaa attacctgct ccactgagcc
    15241 gataccgata ctgcatcgct ccgatatagc ctttctgtgc atcccgccgt aggccaactc
    15301 aatgatcctt ttttggcatt ccccagtaag tttcttgggt ttaagctcga gtggaatatc
    15361 gtttagtagg gcaagtcgcc taaggtagga tcggctttta ccggtaaggc gataaatctc
    15421 actcaatgag cattgctttc gaagcagctg taaacattgc tgctctgtat ttatttcctc
    15481 ttcgagactt ttcaaagata ttggttgtag cgatgctgca caaagatgct tggtaaaaag
    15541 cattttttcc ggctctctaa ataaccagta ggcaaacaat agatgccgaa atgggtggtg
    15601 actggtgtga ggttccaaaa gctgggatag gtatcgatgg tcatagggat ggcgaggaaa
    15661 tggagtatca gagcctggac ggtagtgctt tatttcagca cagaagtcct ccattaaact
    15721 tttacgcctt actcgatgat gagctgtaat ataccccaac tcagacagct tagagcggta
    15781 ggttagtacg gtgtcaacgc gagggatacc gcccgagacc aattgaagaa gattgaagcc
    15841 aaaattggct acctgaacct caacatcaaa agcaactcgt ggatggtcga agagaatggg
    15901 gagtaattcc gcaatgatcc gctgtcgcct aaccagttcc tgcttctcca ggagtacggg
    15961 atgaaatgca caagccgtta cgcctggtat ctgatgtgcc cggtgccaat aggcaacacc
    16021 gtactggcgc aaatcttgtt gagcacacac cgggcaccat ttcaagcaaa gcgaattacc
    16081 acagccaaat aaagacaatt ggcttgctcg atgtgcatct gcgccatcac ctgtcaacat
    16141 tgattgctta agcttatcag cataagtcgg taggaaaaaa agaaacaacg gtgccaacgt
    16201 ttgccgaaac aacattacgt cagcactctc aaggccccat ccggccagtt gctcaagcct
    16261 cgctgtcaga aagggatgga tggacacctt tcttgatcca aagaccttgc tagcaaagct
    16321 atccccttta tagcctgaag ccgtaacata gcggatcagg cgacttagta gtaattcatc
    16381 aggtaggctt acgggcaatt tcattcggta cgcccttagg acttttacgc aaagactttc
    16441 agcggatgtt cacagaaaac gccttcttgc cgcaacaact ccaatggatt ctcctcagcc
    16501 gcagcatgct gtattaaatc aggattaccg attcgttcaa aaaggtcatc cgctgtttgc
    16561 agtatcgtaa gtgcatcggc aaactcagga tggtgaggtc ggctcaggtc accgggtatt
    16621 gattgaggca ctccctcaac tgccaccaat tccttctgct gtgtatgagc catatctgcg
    16681 cgggccggga gtgaagtagt attccgcttc ctttttaacg cagtaattga gcgctctcgc
    16741 ctgatttcat cagtgaccgc cttcgaagcc ttgatagcaa tacttgcggc atgctcaagc
    16801 aactcttccg tgatgcgttc atcgccacaa cctataatgt aacgctgtgc ttcacggaag
    16861 attcgaacgg ccagatctag gttgccaaca gatagctcat aaagcttgtc gttcagctct
    16921 ccagttaatg gagttcttac gttggtccat tggagttccc acaactcttc tataaacagt
    16981 tcccatacac tgtcatt
//
LOCUS       DAMZII010000103        16667 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZII010000103 DAMZII010000000
VERSION     DAMZII010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.103"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            114..1106
                     /gene="tehA"
                     /locus_tag="POA77_003383"
     CDS             114..1106
                     /gene="tehA"
                     /locus_tag="POA77_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDI6366762.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1103..1696
                     /gene="tehB"
                     /locus_tag="POA77_003384"
     CDS             1103..1696
                     /gene="tehB"
                     /locus_tag="POA77_003384"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDI6366763.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            1999..2667
                     /locus_tag="POA77_003385"
     CDS             1999..2667
                     /locus_tag="POA77_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDI6366764.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2685..3186)
                     /gene="tnpA"
                     /locus_tag="POA77_003386"
                     /pseudo
     CDS             complement(2685..3186)
                     /gene="tnpA"
                     /locus_tag="POA77_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            3194..4402
                     /locus_tag="POA77_003387"
     CDS             3194..4402
                     /locus_tag="POA77_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6366765.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(4442..5656)
                     /gene="ydcO"
                     /locus_tag="POA77_003388"
     CDS             complement(4442..5656)
                     /gene="ydcO"
                     /locus_tag="POA77_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDI6366766.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVITQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            5709..6245
                     /gene="sutR"
                     /locus_tag="POA77_003389"
     CDS             5709..6245
                     /gene="sutR"
                     /locus_tag="POA77_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDI6366767.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            6318..8279
                     /gene="rlhA"
                     /locus_tag="POA77_003390"
     CDS             6318..8279
                     /gene="rlhA"
                     /locus_tag="POA77_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDI6366768.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(8371..8601)
                     /locus_tag="POA77_003391"
     CDS             complement(8371..8601)
                     /locus_tag="POA77_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDI6366769.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            8823..8977
                     /locus_tag="POA77_003392"
                     /pseudo
     CDS             8823..8977
                     /locus_tag="POA77_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            9023..9439
                     /gene="hicB"
                     /locus_tag="POA77_003393"
     CDS             9023..9439
                     /gene="hicB"
                     /locus_tag="POA77_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDI6366770.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            9518..10924
                     /locus_tag="POA77_003394"
     CDS             9518..10924
                     /locus_tag="POA77_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6366771.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYVL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            11169..12314
                     /locus_tag="POA77_003395"
     CDS             11169..12314
                     /locus_tag="POA77_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6366772.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            12332..13345
                     /locus_tag="POA77_003396"
     CDS             12332..13345
                     /locus_tag="POA77_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6366773.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            13346..14287
                     /locus_tag="POA77_003397"
     CDS             13346..14287
                     /locus_tag="POA77_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366774.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWSSYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            14277..15071
                     /locus_tag="POA77_003398"
     CDS             14277..15071
                     /locus_tag="POA77_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366775.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            15093..16517
                     /gene="patD"
                     /locus_tag="POA77_003399"
     CDS             15093..16517
                     /gene="patD"
                     /locus_tag="POA77_003399"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDI6366776.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         4105 a         4013 c         4402 g         4147 t
ORIGIN      
        1 cacaggtgac ttactataac cgtagcaaat tctgcggctc tggctatgat cgagaaaatt
       61 ccataaaatg catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga
      121 gcgataaagt gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga
      181 tgggatttgc ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg
      241 ggctggtgat tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac
      301 tcatacgctt tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg
      361 tgagtttgtt tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc
      421 cactggcggt gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc
      481 aaactgcggg attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc
      541 tgccgacagt tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacactg
      601 acgccggtct ggtgttttta ggcgcaggcg ttttctcatg gctaagcctt gaaccggtga
      661 tcttgcagcg tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca
      721 ttcagctcgc tcctgcgctg gtggcttgta gtgcctggct gagcgtcaac ggcggcgagg
      781 gtgacacgct ggcgaaaatg cttttcggtt atggactgct gcaactgctg tttatgctac
      841 gtctgatgcc atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg
      901 gcgtatctgc actggcaacc accggtttgc atctggggag tggcagcgat aatggatttt
      961 tccatacgct ggcggtgccg ctgtttatct ttaccaattt tattattgca atactgctca
     1021 tccgtacttt tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt
     1081 taatgaaagc agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata
     1141 tgaattaacc cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa
     1201 aacgctggat ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta
     1261 tgatgttgac gcatgggata aaaatgccat gagtatcgcc aacgtcgagc gcattaaatc
     1321 cattgaaaat ctggataatt tacacacccg ggtcgttgat ctgaataacc tcacatttga
     1381 tggacagtac gattttattc tttcgactgt ggtgctgatg ttccttgagg ctaaaaccat
     1441 ccccgggttg attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt
     1501 ggcggcgatg gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga
     1561 gggagaatta cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg
     1621 cgagctgcac cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct
     1681 ggcacgtaaa aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga
     1741 tttactgttc ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca
     1801 gtagtcgaag ttttttagca gcttaactta ctgaaattta actactgatg attgacttag
     1861 cccctttttc ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa
     1921 cacttttttt gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat
     1981 catctcagga aactattcat gcgtaccaca tcatttgcga aagttgcagc tttatgcggc
     2041 ttgttggctc tgtctggttg tgcctctaaa atcacccagc cagataaata ttctggtttt
     2101 ttaaacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc tgttttacgt
     2161 tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa cccaatcact
     2221 tattatccgg tgccaaaacc ttctacccag gtagggcaga aagttctgga taaaattttg
     2281 aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt ggttaccacc
     2341 gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac cagcaaagaa
     2401 gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac gcaaatggct
     2461 acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat tgatgcagcg
     2521 actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct gaataacgaa
     2581 agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc gaccgatgcc
     2641 accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat ggcgcttttt
     2701 atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg gttttccacc
     2761 tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg cgcacctccg
     2821 cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata cctgactgca
     2881 atatctggtc gatctcgtcg cagtaaccta ctcgatacgc ctttgaggct gtttaccaga
     2941 ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc tggttcgcca
     3001 tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta agtgcgtagt
     3061 ttttctgtcg cgtcaaaaaa tctggcgtcg gtatctggtg acaaagacca ggtgaacatg
     3121 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tctttattca cagaccaaag
     3181 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac caggtggtca
     3241 acaggagtgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
     3301 ggcacgtcag aatgagaatc atgaggccgg taataaatac atcccttacg ggaaaatggc
     3361 ttcctggctg gttgagtgga aaaatgccac tgaaacgcaa tggcttaaag attctccctc
     3421 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccggaa
     3481 gcgggctgct tttccccgat tcaaaaagcg gggacagaat gatgcattcc gctacccgca
     3541 gggcgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaactgg gctggatgcg
     3601 ctaccggaac agccgtcagg tcacgggtgt tgtgaaaaat gtcactgtca gccagtcctg
     3661 cggtaagtgg tacatcagta ttcagacaga aagtgaagta tccactccgg ctcacccttc
     3721 agcatcaatg gtcgggctgg atgctggcgt ggctaaactc gccacgctgt cagatggcac
     3781 agtctttgag cctgtaaaca gttttcagaa aaaccagaag acgctggcga gacttcagcg
     3841 acagttaagc cgcaaggtca aattcagcaa caactggcag aagcagaaac gcaaaataca
     3901 gcgactgcat tcctgtatcg caaatatccg cagggactac cttcacaaag tcacaacggc
     3961 cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg aaggtcagca acatgtcaaa
     4021 gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc cgggcaaaat caggtttaaa
     4081 ccgttcgata ctggatcagg gctggtatga aatgcaccgc cagcttgagt acaagcagct
     4141 ctggcgtggc ggtcaggtgc ttgctgttcc gccagcgtat acaagccagc gttgcgcgta
     4201 ctgtggtcat acagcgaaag agaaccgcct gtcacaaagt aaattcagat gccaggtatg
     4261 tggatataca gcgaacgccg atgtaaatgg cgctcgtaac attttagcgg cggggcacgc
     4321 cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg ttgaagcagg aacccaccga
     4381 aatgattcag gcgacagcct gaacgtagca gggatccacg tccttcaggg cgtggaggat
     4441 gtcaatatcg gtttctgtca gcgattaaat tcaacaccac gtaacaaacg cctccggcaa
     4501 ttaatcccca gaacgcagaa ccaatcccga ccagcgtcaa tccacttgcc gttaccagaa
     4561 atgccaccac cgccgcgtcg cgctcacgct cattatgcag cgcctgatac aaactgccgc
     4621 caatggtact caacagcgcc agacctgcca gcatctggat ccaacttacg ggcagggcag
     4681 ccatcatccc ggtaatggca ctaccaaaca gacctgcgag caaatagaaa atgcctgcaa
     4741 cggcagctgc cagccaacgt tgatctttat ccggatgcgc ttccgggctt tggcaaatag
     4801 ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa aggggaaaaa accagtgcca
     4861 gcaatccagt aaatacaatt aatggcgaaa caggagccga atatccagct gctttcattg
     4921 ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag aaaaaggggg agtgcaacgc
     4981 tcaggctgtg agcaaacgaa aaatcagggg taatataagt ggggagaacg ggtttaaaga
     5041 caacatcagt tgtgacaacg tcaccttgcg tgatgacgat cacgatccca ataatcatcg
     5101 cggcaattac cgcatagcgc ggcgcaacgg ccttggttgc cagccatacc agcaacatac
     5161 ttccacacaa cgtaaattga ccgtccagac tggcaaacgc ctgtaaacca aagcgtaata
     5221 aaatcccggc aagcattgcc gccgcaagcg agtgcggaat aatgcgcatc agacgagcaa
     5281 agagtcccgt tatgccgcag aggactatta acgcgttggt gacaataaaa acgccgatgg
     5341 cttcgttaag tgttagtccc tgcaatccgg tgaccaacaa agccgcgcca ggcgttgacc
     5401 atgcggtgag aacaggtacg cgataccata atgtcagagt cagcgtactg acgcccattg
     5461 ccagccccag cgccgtcatc cagccagaga tttgtgcagt ggtggctccg gcgacaatcg
     5521 ctgcttgcca gattattgcc gctgaactgg cgtagccaat taataccgcc agaaaccccg
     5581 ccagtagcgt gggtggagga atagaaaaca gacgcatagt cacctcgtgc gttatagcgt
     5641 acaggcaccg tagcatttgt ccgttataac gcacaagtga taaacttccg ttttgccgga
     5701 ggagtcgcat ggaaaatctc gctcgctttt tatccaccac acttaaacaa ctacgccagc
     5761 agcgcggctg gagtctttcg cgactggcag aagcgacggg cgtttctaaa gcaatgttag
     5821 ggcagattga gcgtaatgag tccagtccga cggtcgcgac attatggaaa attgccaccg
     5881 ggttgaacgt accattttca acatttattt ctccgccgca gtccgctacg ccttcagttt
     5941 atgatccaca acagcaggcg atggtgatca cttcgctgtt tccttatgat ccacaactct
     6001 gcttcgaaca cttctcgatt cagatggcat ctggtgcaat aagcgaatcg acgccccatg
     6061 agaagggggt aatcgaacat gtcgtcgtca ttgatggaca acttgatctg tgcgttgatg
     6121 gggaatggca gacccttaat tgtggggaag gcgttcgatt tgctgcagac gtcacgcata
     6181 tctatcgtaa tggtggggag caaaccgtac attttcattc cctcatccat tacccgcgca
     6241 gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc taaaatagcc gccatttttc
     6301 agctactgga taagaatgtg accgtatctt ctcatcgact tgaactgtta agcccggcac
     6361 gcgatgccgc cattgcccgc gaagctattt tgcacggtgc cgatgctgtt tatatcggcg
     6421 gccctggttt tggtgcccgt cataatgcca gtaatagcct gaaagatatt gccgagctgg
     6481 tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac gcttaacacc attttgcatg
     6541 atgatgagct ggaacccgcg caacggctga ttactgacct ctaccagacc ggtgtcgatg
     6601 cgctgattgt tcaggatatg gggattctgg aacttgatat tccgccgatt gaactgcacg
     6661 ccagtacgca gtgcgacatt cgtacagttg aaaaagcgaa gttcctctct gatgttggct
     6721 tcacgcagat tgtgctggcg cgagagctga atcttgagca gatccgcgcg attcaccagg
     6781 ccacggacgc gaccattgaa ttctttattc atggggcact gtgcgtggcc tattcgggtc
     6841 agtgctacat ttctcatgcg caaacagggc gtagcgccaa ccgtggtgat tgctcgcagg
     6901 cgtgccgttt gccatacaca ttgaaagacg atcaggggcg ggtggtttcc tatgaaaaac
     6961 atctgctgtc gatgaaagat aacgatcaga ctgccaacct cggcgcgctg attgatgctg
     7021 gcgtacgctc cttcaagatt gaagggcgtt acaaagatat gagctacgtg aagaatatca
     7081 ccgcccatta tcgccagatg cttgatgcca ttattgaaga acgtggcgat ctggcgcgcg
     7141 cttcatcagg tcgtactgaa catttctttg ttccatcgac ggaaaagact ttccaccgtg
     7201 gtagcacaga ttattttgtg aatgcccgta aaggcgatat tggcgcgttc gattcgccga
     7261 aatttatcgg cctgccggta ggcgaagtat tgaaagtggc gaaagatcat ctcgatgttg
     7321 ccgtcaccga gccactggca aatggtgatg gcctgaacgt gatgattaaa cgtgaagtcg
     7381 tcggttttcg tgccaatacg gtcgagaaaa ccggagaaaa tcagtaccgc gtctggccca
     7441 atgaaatgcc agcagatttg cacaaaattc gtccacatca cccactaaac cgtaatcttg
     7501 atcataactg gcagcaggca ctgacaaaaa cctccagcga acgtcgggtg gcggtagaca
     7561 ttgaactggg cggctggcag gaacaactga ttctgaccct caccagtgaa gagggtgtca
     7621 gcatcacgca tacgctggac gggcagttcg acgaagccaa taacgccgaa aaagcaatga
     7681 acaatctgaa ggatggtctg gcaaaactgg ggcaaaccct ctattacgcc cgcgatgtgc
     7741 aaattaattt gccgggggcg ctgtttgtac caaacagtct gttaaaccag ttccgccgtg
     7801 aagctgctga catgctggat gctgcgcgtc ttgccagtta ccagcgcggc agccgtaaac
     7861 cggttgctga tcctgcgccg gtttatccgc aaacgcatct gagtttcctc gcgaacgtat
     7921 acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt gcagctgatt gacgcggcgt
     7981 atgaagcaca tgaagagaag ggcgaagtcc cggtgatgat caccaagcat tgtctgcgct
     8041 ttgcctttaa tctgtgcccg aaacaggcga aaggcaatat caaaagctgg aaggcgacgc
     8101 caatgcaact ggttaacggc gatgaagtat taacgctaaa gtttgattgc cgcccatgcg
     8161 agatgcacgt cattggcaaa atcaaaaatc acatactgaa aatgccgtta ccgggaagcg
     8221 tagtggcatc cgtaagtccg gatgagctgc tgaaaacatt gcctaagcga aaagggtaaa
     8281 acgccagttt tctggttact cacaactcat tgaatctgca tgatattgcc tgccgggtaa
     8341 ggcgtctcgc cgcatccggc aacaaatgac tcaacgttgt cccgcttctg gtttgcgcga
     8401 tttttgccag taaaaatgct cgcgcaaacc ttccgccgac tcttccgcca cagcacgcaa
     8461 ttcatcgctg tccgcttcat gacgcagctg atgatccaca ttctttaccc acacaaattc
     8521 atgtcctttg tgccctgcca tgagttgtcc tgagaacagc gcacacgtta ataagacaac
     8581 cgataacgcc ttcgtaaaca tcctgccacc tttttcttac cttttgccgc tatgatgccg
     8641 atcgtttctt gaggttatta ttcagttttg caaattagcg caaagaaatt ctggaatctt
     8701 ccttcctgat tttgcatggc attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa
     8761 ttacacacgg aggtaaacaa taatgtttac tttgttgata tactcagcgg cagggaggcg
     8821 atgtgaaaca aagcgagttc agacgttggc tcgaatctca gggcgtcgat gtagcgaatg
     8881 gccgcaacca tttgaaactc aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct
     8941 gcgtaaagca atcctgaaac aactcggttt gagttaatcg ccaattaaaa aggttaatga
     9001 catgcgagag acagtcgaaa ttatgcgtta tcccgtcact cttacacccg cgccggaagg
     9061 cggttatatg gtttcttttg tggatatccc tgaagcgttg acgcagggcg aaactgtcgc
     9121 tgaagcgatg gaagcggcaa aagatgcttt actgaccgca tttgattttt attttgaaga
     9181 taacgagctt atcccgttac cttcgccatt aaatagtcat gatcacttta ttgaagtacc
     9241 tttgagcgtc gcctctaagg tattgctgtt aaatgctttt ttacagtcag aaatcactca
     9301 gcaagagtta gccaggcgaa ttggcaaacc taaacaggag attactcgcc tatttaactt
     9361 gcaccatgcg acaaaaatcg acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt
     9421 atcgctggtg atggtttaat tacagttaac gaaaagttgt catttttaac aactgatata
     9481 gactgccgaa tcatctgcac ataattacga ttcgataatg aaaaaatacc agcagcttgc
     9541 agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc
     9601 gttgcgtgac caggtggcgc tttcaggcat gagctttatg actgtcagcc atgcctatca
     9661 gttgctcgaa agtcagggat atattatcgc acgaccgcag tcgggttatt acgttgcgcc
     9721 acaggcaata aaaatgccga aagcgccagt cattccagtc actcgagatg aagcagtcga
     9781 tatcaacact tatatttttg atatgttgca ggccagtcgc gatccgtcgg tcgttccgtt
     9841 tgcctcggcc tttcccgacc cgcgactttt ccccctccaa caactaaacc gctcgctggc
     9901 gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa aacttaccgc caggaaacgc
     9961 agaactgcgt caggctattg ctcgtcgcta tgtcttacag ggcatcacca tttctcctga
    10021 tgaaattgtc attactgccg gggcgttaga ggcattaaac ctcagtttgc aagcggtaac
    10081 tgaaccgggc gattgggtga tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct
    10141 ggagcggcta cggctgaagg cgttatcggt ggcgacggat gttaaagaag ggatcgatct
    10201 tcaggcgctg gaactggcgt tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa
    10261 tagccagaac ccactcggat ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt
    10321 actcaatcag tacaacgtaa cgctgattga agatgacgtt tacagcgaac tttattttgg
    10381 acgggaaaaa ccgctgcctg cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc
    10441 ttcgttttcg aaatgtctgg tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca
    10501 tgcacgtaaa attcaacgct tgcagttgat gagtacgctt tccaccagct caccgatgca
    10561 acttgcgctg gtggattacc tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg
    10621 tcgccagctt gcggaacgta aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc
    10681 ggaagttaaa attcatcata gtgacagcgg ttattttctc tggctggagc tccccgagcc
    10741 gttagatgcc ggcgaattaa gcctggcggc actgacgcat catatcagta ttgcgccggg
    10801 taaaatgttt tctaccggtg aaaactggtc acgttttttt cgttttaata ccgcgtggca
    10861 gtggggagaa cgtgaagaac aggcggtaaa acaattaggc aaacttattc aagaacggct
    10921 gtaatagcgt ttaatttaat tcctcttaga ttgggtaata tgaatttcga atagcagtca
    10981 tatttcctaa ccccttgtct atactccaga agataacctt acagacggca taatgcgcgg
    11041 tagctcacaa cctgattaaa ttttctcagg ggcgaaggtg tgcctgcaag ccgccgtcta
    11101 tggttaaaca aggagatatt tttacggcac ggcggctgaa caattaatta cgacaggagt
    11161 aagaccttat gagcaagaca tttgcccgca gcagcctgtg tgcgctcacc atgacaataa
    11221 tgaccgctca cgccgccgaa ccacctacca atttagataa accggaaggg cgactggata
    11281 ttatcgcctg gccgggatac atcgaacgcg gacaaactga taaacaatac gactgggtaa
    11341 cgcaatttga aaaagagaca ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg
    11401 aaatggtcag tctgatgacc aaagggggtt acgatctggt tacggcatcc ggcgatgcct
    11461 cgctgcgttt gattatgggc aaacgcgtgc agccgattaa taccgccttg attcccaact
    11521 ggaaaacgct cgatccgcgc gtggttaaag gcgactggtt taatgttggc ggcaaagttt
    11581 acggcacacc ttaccaatgg gggccgaacc tgctgatgta caacactaaa accttcccga
    11641 cgccgccgga tagctggcaa gtggtttttg ttgagcaaaa tctgccggac ggcaagagca
    11701 ataaaggccg cgttcaggct tatgatggcc ctatctacat tgcggacgct gcgttgttcg
    11761 ttaaagccac tcagccgcag ttgggcatca gcgatccgta tcaactaacc gaagaacagt
    11821 accaggcggt gctgaaagtg ctgcgcgctc aacacagttt gatccatcgc tactggcatg
    11881 acactaccgt gcaaatgagc gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc
    11941 cctatcaggc caacgccctg aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg
    12001 agggtgttac cggttgggct gataccacca tgctgcatag cgaagcgaaa catccggttt
    12061 gcgcctacaa atggatgaac tggtcattaa cgccaaaagt gcagggcgat gtggcggcct
    12121 ggtttggctc gttaccggta gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa
    12181 aaggttgtga aaccaacggt tttaactatt tcgacaaaat cgccttctgg aaaacgccta
    12241 tagcagaagg gggcaagttt gttccctaca gtcgctggac gcaggattac attgccatta
    12301 tgggcggtcg ctaacttcgc tggggtgctt tatgacgtac gcagtggagt ttgacaacgt
    12361 ctcgcggttg tacggtgacg tgcgcgcagt agatggcgtc agtattgcga taaaagatgg
    12421 tgagttcttc tctatgctgg ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat
    12481 tgctggcttc gaacagcttt ccggcggggc tatctctatc tttggtaaac ccgccagcaa
    12541 tctgccaccg tgggagcggg acgtgaatac tgtctttcag gactacgcgc tatttccgca
    12601 tatgtcgatt cttgacaatg tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca
    12661 gcggcacgca atggcgcaag aggcgctgga gaaagtggcg ttggggtttg tacatcaacg
    12721 taaaccgtca caactttctg gtggtcagcg ccagcgggtt gctatcgcca gagcattggt
    12781 gaatgaaccg cgcgtattgc tgttggatga accgctcggc gcactggatc tcaaattgcg
    12841 tgagcagatg cagctggaac tgaaaaaact gcaacagtct ctcggtatca cttttatctt
    12901 cgttacccac gatcagggcg aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa
    12961 tggacgcatt gagcaggtcg attccccgcg cgatctctat atgcgcccgc gcacgccgtt
    13021 tgttgccgga ttcgtcggga catccaacgt ttttgatgga ctgatggcag agaaactttg
    13081 tggcatgacg ggaagcttcg ccctgcgacc ggaacatatc cgcctcaaca cccctggtga
    13141 actgcaggcc aatggcacga tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga
    13201 actgaaattg aacggcggtg aaaaactgct tgtgagtcag gccaatatga caggcgaaga
    13261 actgcctgcc acgctcacgc ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat
    13321 ggtgccgctg gttgaggaga ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc
    13381 aggtctgggt aaggtgtccg gtttcttctg gcgtaatccg gggctggggc tgtttttact
    13441 gctgcttggc ccgctaatgt ggtttggcat tgtctatttc ggctcgctgc tgacactgtt
    13501 atggcaggga ttttatactt ttgacgattt caccatgtcg gtaacgccgg aactgacgct
    13561 ggcgaatatc cgtgcgctgt ttaatccggc gaattacgac atcattctcc gcacgctgac
    13621 tatggctgtg gcagtcacta tcgccagcgc cattctggct tttccaatgg cgtggtatat
    13681 ggcgcgctat accagcggga aaatgaaagc ttttttttat attgcggtaa tgttgccgat
    13741 gtggtcgagc tacattgtta aagcctatgc ctggacgtta ttgctggcaa aagatggcgt
    13801 ggctcagtgg tttttacaac atcttgggct ggaaccactg ctgactgcgt tccttacatt
    13861 acctgcggtg ggcggaaata cgctgtcaac ttccgggctg gggcgctttc tggtgtttct
    13921 ctatatctgg ttgccgttca tgatcctgcc cgttcaggcg gcgcttgagc gtttgccgcc
    13981 gtcattgttg caggcgtcgg ctgatctcgg cgcacgtcca cgacaaacct ttcgctatgt
    14041 ggtgctgccg ctggcaatcc cgggtattgc cgctggctct atctttacct tctcactcac
    14101 actgggcgat tttatcgtcc cgcagctggt tggtcctcca ggatatttta tcggcaatat
    14161 ggtttattcc cagcaggggg cgattggcaa tatgccgatg gcggcggcat tcaccctggt
    14221 gccgattgtt ctcatcgcac tgtacctggc gttcgtgaaa cgtctgggag cgttcgatgc
    14281 actctgaacg cgcaccgttt ttcctcaaac tggcggcctg gggcggcgtg gttttcctac
    14341 attttcccat cctgattatc gccgcttatg cgtttaacac tgaagatgcg gcgtttagtt
    14401 ttccaccgca gggcctgacg ctgcgctggt ttagcgtggc agcacagcgt agtgatattc
    14461 ttgatgccgt gacactgtca cttaaagtgg cggcgctggc gacattaatg gcgctggtgt
    14521 tagggacgct ggcagctgcc gcgctgtggc gacgagactt tttcggcaaa aacgccattt
    14581 cgctgttact gctgctgccc attgcgctgc cgggcattgt cactggtctg gcgctattaa
    14641 ccgcttttaa aaccatcaat ctggagccgg gatttttcac catcgtggtc ggtcatgcga
    14701 ctttttgcgt agtggtggtg tttaacaatg tcatcgcccg ttttcgccgc acctcgtgga
    14761 gtctggttga ggcgtcaatg gatcttgggg ccaatggctg gcaaaccttc cgctacgtag
    14821 tgttgccgaa tctcagttcg gcgctactgg caggaggaat gctggcgttt gccttgtcgt
    14881 tcgatgaaat catcgttacg acttttacgg caggtcatga acgaacgtta ccgttgtggt
    14941 tgctcaatca gcttgggcga ccgcgtgatg taccggtaac taacgtggtg gcactgctgg
    15001 ttatgttggt aacaaccttg ccgatcctgg gggcctggtg gctaacccgc gaaggcgaca
    15061 atggtcaata accactgata caggaatatg ctatgcaaca taagttactg attaacggag
    15121 aactggttag cggcgaaggg gaaaaacagc ctgtctataa tccggcaacg ggggacgttt
    15181 tactggagat tgccgaggca tccgcagagc aggtcgatgc tgctgtgcgc gcggcagatg
    15241 cagcatttgc cgaatggggg caaaccacgc cgaaagtgcg tgcggaatgt ctgctgaaac
    15301 tggctgatgt tatcgaagaa aatggtcagg tttttgccga actggagtcc cgtaattgtg
    15361 gcaaaccgct gcatagtgcg ttcaatgatg aaatcccggc gattgtcgat gtttttcgct
    15421 ttttcgcggg tgcggcgcgc tgtctgaatg gtctggcggc aggtgaatat cttgaaggtc
    15481 atacttcgat gatccgtcgc gatccgttgg gggtcgtggc ttctatcgca ccgtggaatt
    15541 atccgctgat gatggcagcg tggaaactgg ctccggcgct ggcggcaggg aactgcgtag
    15601 tgcttaaacc atcagaaatt accccgctga ccgcgttgaa gttggcagag ctggcgaaag
    15661 atatcttccc agcaggcgtg attaacgtac tgtttggcag aggcaaaacg gtgggtgatc
    15721 cgctgaccgg tcatcccaaa gtgcggatgg tgtcgctgac gggctctatc gccaccggcg
    15781 agcacatcat cagccatacc gcgtcgtcca ttaagcgtac tcatatggaa cttggtggca
    15841 aagcgccagt gattgttttt gatgatgcgg atattgaagc agtggtcgaa ggtgtacgta
    15901 catttggcta ttacaatgct ggacaggatt gtactgcggc ttgtcggatc tacgcgcaaa
    15961 aaggcattta cgatacgctg gtggaaaaac tgggtgctgc ggtggcaacg ttaaaatctg
    16021 gtgcgccgga tgacgagtct acggagcttg gacctttaag ctcgctggcg catctcgaac
    16081 gcgtcagcaa ggcagtagaa gaggcgaaag cgacagggca catcaaagtg atcactggcg
    16141 gtgaaaagcg caagggtaat ggctattact atgcgccgac gctgctggct ggcgcattac
    16201 aggacgatgc catcgtgcaa aaagaggtat ttggtccagt agtgagtgtt acgcccttcg
    16261 acaacgaaga acaggtggtg aactgggcga atgacagcca gtacggactt gcatcttcgg
    16321 tatggacgaa agatgtgggc agggcgcatc gcgtcagcgc acggctgcaa tatggttgta
    16381 cctgggtcaa tacccatttc atgctggtaa gtgaaatgcc gcacggtggg cagaaacttt
    16441 ctggttacgg caaggatatg tcactttatg ggctggagga ttacaccgtc gtccgccacg
    16501 tcatggttaa acattaagtt agatgttagt ggctgttata ggacggccac tatcgtttta
    16561 ggttctgagt ttaatggcgg ttaagtatta gaatgattac attaataact agcgtaaaaa
    16621 ttaggctaaa tgtttgatgg cgatttttca tcctgtcaca gttgatt
//
LOCUS       DAMZII010000104        15834 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZII010000104 DAMZII010000000
VERSION     DAMZII010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.104"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            104..1186
                     /gene="ompC"
                     /locus_tag="POA77_003400"
     CDS             104..1186
                     /gene="ompC"
                     /locus_tag="POA77_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6366777.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRNEVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNGLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEA
                     VAQYQFDFGLRPSVAYLHSKGKDLGVWGDQNLVEYVDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1375..1758)
                     /gene="quuD"
                     /locus_tag="POA77_003401"
     CDS             complement(1375..1758)
                     /gene="quuD"
                     /locus_tag="POA77_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDI6366778.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1844..1984)
                     /locus_tag="POA77_003402"
     CDS             complement(1844..1984)
                     /locus_tag="POA77_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDI6366779.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1981..2343)
                     /gene="rusA"
                     /locus_tag="POA77_003403"
     CDS             complement(1981..2343)
                     /gene="rusA"
                     /locus_tag="POA77_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HDI6366780.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRCRRDLDNLQKAAFDALTKAGFWLDDELVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(2340..2630)
                     /locus_tag="POA77_003404"
     CDS             complement(2340..2630)
                     /locus_tag="POA77_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDI6366781.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2623..2793)
                     /locus_tag="POA77_003405"
     CDS             complement(2623..2793)
                     /locus_tag="POA77_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366782.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2793..3248)
                     /gene="ybcN"
                     /locus_tag="POA77_003406"
     CDS             complement(2793..3248)
                     /gene="ybcN"
                     /locus_tag="POA77_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDI6366783.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKELVDVVTGEITTI
                     QSLRHTSNLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3245..3346)
                     /locus_tag="POA77_003407"
     CDS             complement(3245..3346)
                     /locus_tag="POA77_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366784.1"
                     /translation="MRTYNPTSLLHSQMQKCTCDILHPAFDLCGGEA"
     gene            complement(3441..4223)
                     /locus_tag="POA77_003408"
     CDS             complement(3441..4223)
                     /locus_tag="POA77_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366785.1"
                     /translation="MDNINKHGLSRRIPETIKRQIRQRCGFGCVICGFGFYDYEHFKP
                     DFVDAKIHDPNGMTLLCSQCNQKRARGRLSAQTVEIADRNPKCLQAGFANEMFDFHNE
                     PITVKFAGVTFHNCQNLIVVNEQPILSVKPSPIPHGPMLLSGIFCNSIGKETLLIDEN
                     EWKAKSDNWDVECTGPRITIRRGPGEFALVLKMEPPTGLIVERLDMLYEGVRMKGDKD
                     LLEVSINGGPLHRWQSCSMSNCHTGLAIQGGIRAANDPLYCA"
     gene            complement(4398..4721)
                     /locus_tag="POA77_003409"
     CDS             complement(4398..4721)
                     /locus_tag="POA77_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366786.1"
                     /translation="MDAGVPVLAVQQFPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPQRLNRARAILARETAHVKLQPWQDDKWSQVLPHLP
                     QNLFQ"
     gene            complement(4833..6359)
                     /locus_tag="POA77_003410"
     CDS             complement(4833..6359)
                     /locus_tag="POA77_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6366787.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSNYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     AALSVAPEVTAIAEKIRVLDKELRRASVSLKTLKSKAVSSLGDFHAIDLTSKNGRELC
                     RTLAYKTFEKIIINTDNKTCDIYFMNGIVFKHYPLMKTISAQQAISTLKYMVDGEVYF
                     "
     gene            complement(6417..6566)
                     /locus_tag="POA77_003411"
     CDS             complement(6417..6566)
                     /locus_tag="POA77_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366788.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWREKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            complement(6615..6947)
                     /gene="emrE"
                     /locus_tag="POA77_003412"
     CDS             complement(6615..6947)
                     /gene="emrE"
                     /locus_tag="POA77_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDI6366789.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(7015..7317)
                     /locus_tag="POA77_003413"
     CDS             complement(7015..7317)
                     /locus_tag="POA77_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HDI6366790.1"
                     /translation="MTGKEAIIHYLGTHKKFCAQDVAAVTGATVTSINQAAAKMARAG
                     LLAIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(7314..8015)
                     /locus_tag="POA77_003414"
     CDS             complement(7314..8015)
                     /locus_tag="POA77_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDI6366791.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            complement(8012..8911)
                     /locus_tag="POA77_003415"
     CDS             complement(8012..8911)
                     /locus_tag="POA77_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDI6366792.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGMIASKPKLDLTNTDWIYGVDL"
     gene            complement(8944..9238)
                     /locus_tag="POA77_003416"
                     /pseudo
     CDS             complement(8944..9238)
                     /locus_tag="POA77_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
     gene            complement(9358..9573)
                     /locus_tag="POA77_003417"
     CDS             complement(9358..9573)
                     /locus_tag="POA77_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6366793.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            9679..10311
                     /locus_tag="POA77_003418"
     CDS             9679..10311
                     /locus_tag="POA77_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDI6366794.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGESVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            10308..10712
                     /locus_tag="POA77_003419"
     CDS             10308..10712
                     /locus_tag="POA77_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366795.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGAMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTNK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            11066..11389
                     /locus_tag="POA77_003420"
     CDS             11066..11389
                     /locus_tag="POA77_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDI6366796.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPGERGITCRGRQKIKGK
                     SISLI"
     gene            complement(11382..11876)
                     /locus_tag="POA77_003421"
     CDS             complement(11382..11876)
                     /locus_tag="POA77_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366797.1"
                     /translation="MSDEKGIISRITDAVSGAGGALMSAVGAVKEIQKMQIDYSVKEK
                     TYELVDKLMDAQQQQMSLNELLMISKDKIIELEEKINRASKWEEEKKNYEMYTPTVAT
                     VVYRLKKSANTGQPMHYLCAQCYESSVKSILQYEGFAPPSNHRMRCHRCNASYLFPKS
                     AFSK"
     gene            12133..12501
                     /locus_tag="POA77_003422"
     CDS             12133..12501
                     /locus_tag="POA77_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HDI6366798.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            12574..12738
                     /locus_tag="POA77_003423"
     CDS             12574..12738
                     /locus_tag="POA77_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDI6366799.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            12707..12871
                     /gene="kil"
                     /locus_tag="POA77_003424"
     CDS             12707..12871
                     /gene="kil"
                     /locus_tag="POA77_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDI6366800.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIHYPPSLFS"
     gene            12925..13221
                     /locus_tag="POA77_003425"
     CDS             12925..13221
                     /locus_tag="POA77_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDI6366801.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKSLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            13227..14012
                     /gene="bet"
                     /locus_tag="POA77_003426"
     CDS             13227..14012
                     /gene="bet"
                     /locus_tag="POA77_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDI6366802.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            14009..14689
                     /locus_tag="POA77_003427"
     CDS             14009..14689
                     /locus_tag="POA77_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDI6366803.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDENMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            14686..14868
                     /locus_tag="POA77_003428"
     CDS             14686..14868
                     /locus_tag="POA77_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDI6366804.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            14841..15032
                     /locus_tag="POA77_003429"
     CDS             14841..15032
                     /locus_tag="POA77_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDI6366805.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            15109..15324
                     /locus_tag="POA77_003430"
     CDS             15109..15324
                     /locus_tag="POA77_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6366806.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            15423..15644
                     /locus_tag="POA77_003431"
     CDS             15423..15644
                     /locus_tag="POA77_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI6366807.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT         4386 a         3652 c         3432 g         4364 t
ORIGIN      
        1 gagaaactaa aacttacatc ttgaaataat cacattgatt agatgaatat ttatcgcgca
       61 gtgacatcat tttttaataa tagttcaaaa aaaagggctc acgatgaaaa aattaacagt
      121 ggcaatttct gctgtagctg catcagtact gatggcgatg tctgctcagg cagctgaaat
      181 ttataataaa gacagtaaca agctggatct gtacgggaaa gttaatgcta agcactactt
      241 ctcctctaat gatgcagatg atggtgatac tacttatgcc cgtcttggct tcaaaggtga
      301 aacccaaatc aacgatcaac tgactggttt cggtcagtgg gaatatgaat tcaaaggcaa
      361 ccgcgctgaa tctcaaggtt cctccaaaga caaaacccgt cttgcatttg caggcctgaa
      421 attcggtgac tacggctcaa tcgattacgg ccgtaactac ggtgtagcat acgacatcgg
      481 tgcgtggact gacgttctgc cagaattcgg tggcgatacc tggacccaaa cagatgtatt
      541 catgactggt cgcaccacag gtgttgcaac ttatcgtaac aacgacttct ttggtctggt
      601 tgatggcctg aactttgcag ctcagtatca gggcaaaaat gaccgaaatg aagtaactga
      661 agctaatggc gatggtttcg gtttctcaac tacttatgag tatgaaggat tcggcgtggg
      721 tgcaacctat gctaaatctg atcgcactaa taatcaggtt atctacggta acaacggtct
      781 gaatgcttct ggtcaaaatg ctgaagtatg ggcagctggt ctgaaatatg atgcgaacaa
      841 catctatctg gccaccacct attctgaaac ccagaacatg actgtttttg gtaataacca
      901 tattgccaac aaagcacaaa acttcgaagc tgttgcacaa tatcagttcg acttcggcct
      961 gcgtccatcc gttgcttacc tgcattctaa aggaaaagac ttgggtgttt ggggtgatca
     1021 gaacctggtt gaatatgttg atgtaggtgc aacctattac ttcaacaaaa atatgtccac
     1081 ttttgttgac tacaaaatca acctgattga taagagcgat ttcacgaaag catctggcgt
     1141 tgctaccgat gatatcgttg ctgtaggtat ggtttaccag ttctaatttg attactaaaa
     1201 gatatgttgc gggaggcttt gcctccccaa catataagtg gctccctcaa gccacttcct
     1261 ttaggagcac aaccttgctt ctaactatat aaaccttctg ttatatatta ccctttattt
     1321 ttgggggcgt ctcaacgccc catttttaat aatttttagt aaacaattgg catattaatt
     1381 agagttatta acaacgatat ccatctctaa ccggatatct aatgccatta acatcccttc
     1441 aattataccc tcagccttct gtaacctttt cccgatataa ccatcagagc agcaatgctt
     1501 acctgccagt gacatgaatg tcataccgac tacataataa tctactaata aatcgtgcaa
     1561 atcgctgttg ttctttttca gacgggccat gcacccgcaa atgatcatcg cgtcatcgtc
     1621 acaacattgc gggcgagatt ttacttttga aggaattaat cccttaaaac cggcggcaat
     1681 ggacgaccag gtcacatctt catgattatt agccgcccac gctccccaac gctcaagaac
     1741 catctgaata tcacgcatca acttactcca caaaaatcag accagaacgc caattacaag
     1801 caaaaatcaa taaaacagta ttagttgatt gttatctctg acttcatact cctgctcctg
     1861 tcagtgtttt ggcgtaattc ttcagtattc ggtaatcggt caaaacagaa ccggggaaac
     1921 gatataagcg cagacgcccc cagcggtggc gaagaagttc tgccatatta aactcaaaca
     1981 tcattcatct cccagttcag tgatggtcag ttccagcttc ccacctttgg taacaggcat
     2041 cttcacaacg cggtaatcaa cgaccagctc atcatccagc cagaaacctg ctttagtgag
     2101 tgcgtcaaaa gcggcttttt gcagattatc caggtcacgg cgacagcgat ccggcatgtg
     2161 gcactcaatg cggattttca caggcatagc caggccgata tccagcattg cgtttttaat
     2221 gattcgggcg acgttatcgc ggtatgcctg cccctctgcg ctgacgtgcg tgcgcccgcg
     2281 attatggcgg taatagcgat tattgctcgg aggccagggt aatgtgatgc tgtaggtatt
     2341 cacgccttaa taaccccctc tttcagccag ataacctgtg ttctcgccat accttccagc
     2401 gcgcattctt ttgcatatgc agcatcgaca aaatgtgtgc ggcggtcgat ttcgtcgtgg
     2461 caggcagaac atgcaatggt ggcaatcagg tctggcggtt tggtaccggt gccgcacaat
     2521 ccagtcagcc ggatatgtgc cagtacagac gtttcagggt tgccattaca tacgccaggg
     2581 attcttacct ggcattcccg accacgcgct gcttttctca aatcagccat gattcctcct
     2641 tgctgccagt cgcaaccatt ttttatcaac caggctggcg gtatatccga gcagtgttgg
     2701 tatttcggat ggtttcagct caggcttacg cttacgacga tttggtactc tgtagatgtg
     2761 tccgttcatg acacgaataa gcggtgtagc cattacgcct cctgcttgtc gcgcagcagc
     2821 tggaactcgc agctctgcgg aatagtcagg tggcagccaa tattcatcgc ccaggcttca
     2881 accttacaca ggaagacata catctctccg gtatcaagat tggaggtatg acgtaatgac
     2941 tgaatggtgg tgatttcacc ggttacgaca tcaaccagtt ctttggtttc ataaccgaga
     3001 taagtgtgtt tgagagcatc ttttacccaa gctggagtgg cgaacgtttt acccctgctg
     3061 atgaggtatt cactgatttc gctgtaccac atgtggctga gtgcattctg ggaaagactg
     3121 cgtctctcgc gccacggttt aagcaccatg cgaaagcatt tgccgtcctc cagataaggc
     3181 tggatctgcc gaccgatagc ggtgaagtta ccgcgatgca atttgatacc atcttgtggg
     3241 aggttcacgc ttcacctccg cagagatcaa acgctggatg caaaatatcg caggtgcatt
     3301 tctgcatctg tgaatggaga agagaggttg gattgtatgt gcgcataaac gtccccgttt
     3361 agcgcagaag tcaccggagg agttcaaact ccggtgactt aattatggca agttgattgt
     3421 tgaaaatcaa tttagtggat ttaggcgcaa tacaaaggat cattagcagc tctaataccg
     3481 ccctgaatcg caagccctgt atggcaattt gacattgagc atgattgcca tctgtgcaag
     3541 ggacctccat taatcgaaac ttccagcaga tctttatctc ctttcatcct gaccccttca
     3601 tacaacatat caagacgctc aacaatcaat cctgttggag gttccatttt tagaacgagg
     3661 gcaaactctc ctggcccacg tcgtattgtt atacgcgggc cggtacactc cacatcccaa
     3721 ttgtctgatt tagctttcca ttcattttca tctatcagta aagtctcctt accaatagaa
     3781 ttacagaaaa ttcctgataa aagcatcggt ccatgtggta tgggtgaagg tttcacagaa
     3841 agaatgggtt gttcgttgac tacaatcaaa ttttgacagt tatgaaacgt cactcctgca
     3901 aattttactg ttataggctc attatgaaaa tcaaacattt catttgcaaa accagcctgt
     3961 aagcacttgg gatttctgtc agctatttcc actgtctgtg ctgaaagtct accacgagct
     4021 cttttttgat tgcattggga gcaaagaagt gtcattccat tcggatcatg tattttggca
     4081 tcaacgaagt caggtttaaa atgctcataa tcataaaatc caaatccaca aattacacag
     4141 ccaaagccgc acctttgcct tatctgacgt tttattgtct caggtattct tcttgagagc
     4201 ccgtgtttat taatattgtc catatggtta gtacgctata agtttgtttc tataatttta
     4261 ccacaataga taagtttgta tcagaaatcg ctatatggag tgagcggaat atttatatta
     4321 ttcatgcaat tgctctcccg tacgcagccc gcacttccgt catcgcagaa tgacggaagc
     4381 cttactaact agcgccttta ttgaaacaga ttctgaggta aatgcggcaa tacttgactc
     4441 cacttatcat cctgccacgg ctgaagtttt acatgtgccg tttctcgggc gaggattgct
     4501 cgcgctctgt tgagtctctg gggatattct tgctcgatgg aagtgaagtg accagcctca
     4561 ccatgctccg catcctgaag aagatgagta acgttctggt aggcaattaa catcacattc
     4621 cctgctcgcc ataaccaggc gagtgtgcaa agttcgttat cagtgaattg ttttgtgatt
     4681 gggaattgtt gaactgctag aacgggaacg ccagcatcca ttggcagtcc ctatagtaaa
     4741 accatagctc aggacgcttc gttcaggata gatactttta ttgtacttac ctaactttct
     4801 tactatagca cggttggaaa agtgattatt actcaaaaat aaacctcacc atcaaccata
     4861 tatttgagag tacttatcgc ctgctgggcg gatattgttt tcattaaagg atagtgttta
     4921 aaaacaatgc cattcataaa atagatatca caggttttat tatctgtatt gattatgatt
     4981 ttttcgaatg ttttataggc aagtgtacga catagctctc gcccattttt actggttaag
     5041 tcaatagcat gaaaatcacc aagtgaactc accgctttac tcttcaaagt ttttaatgat
     5101 accgaagccc ttcgtaattc cttatctaat actctgattt tttctgctat agcggtaact
     5161 tcaggcgcaa cagataatgc agcaattaaa ttattaattt tcatctggag ctcaataatt
     5221 ttcaactcta aagtttcatt agcatctttc ttgttttcaa ctggttggat tttactacaa
     5281 ttaaaaagca actcattaat gatattataa tcaaccaaat ctctctttat tgatggcctg
     5341 tcacatcgat gcagtcttct catcggacaa acataatagc catgcaaact tccagatacc
     5401 gcatgaacaa tcatggtatt accacaagcc tcgcacttca taactgttcg aagtagattt
     5461 atcaacatag gatttttgct actattgcta ataccaaaag gtgccaaccg aatttcctgc
     5521 acagcgtaaa acaaatcatc tgatatgact ctgggataat agccagcgat ttcacttatt
     5581 cctttacctc ttgcacgata tgaaggtacg catataccta tcagagcttt attcgctaat
     5641 aatttttcaa ttacagaagg cccccatgca ctttctttgc ctgagaaatt ttttacagca
     5701 tgatcattta aatacttggc tattgcattc aatgatcgcc tttccatcct gagtttaaaa
     5761 attagctcaa tcgttttcac cctgtcgggg tctggaataa aagctgttct tttgtcatcc
     5821 aatgagagcc atctcggaca agacgccgtc ataatcgtgc ctgactccag tgcatcctgc
     5881 cgttttttct tccatgataa tttaacccga cttgacttta tctcgctttc ttcatttgcc
     5941 ctttgtgcta taagtatggc ttttattaat gaatatggat cattcaaaga gtcaatgtta
     6001 tagactgtat tatcgcaaag agttataaca tcaataccgt gattcaaaat caatttcaga
     6061 cgctcaatcg cttcaccgac tttttctctt gaaagtctgt ccaaactttc aactaacaat
     6121 gtagttcctg gcaatatata accatgctct atagcatcta aaaattccga aaaagctcct
     6181 gattgtgcat gctttccttt gaatgcactt aatcctaaat cttcatatgt tatggtatca
     6241 agataataat tactatttac ctttaaccat tcagcaataa gtcttctctg tcggttcaat
     6301 gagtcgccag acatctgacc tggtgatgaa aatcgcatat atgctatggc ttttttcatg
     6361 gtgacacctg ctaacgtatg cttttataaa ccttagtggt gagatataac ttttgtttaa
     6421 tttttattta aaaagacaat taaggtcaca ttatcttgaa tatacaacaa taatcgtatt
     6481 gcaattttct cacgccataa tcttgaaagc acaaaagaat acataaaaaa taaagacatt
     6541 aacaaaaagc ataaaacgag gctcatataa atataagagc ctccatattt tagtcgttta
     6601 aaaacaaatt atttttaatg tggtgtgctt cgtgacaata aattaataac caacacaccg
     6661 gcacaaatca acatcatgcc tataatggct ggcaggtcca gccgttggcc gaaaagtccc
     6721 catgacagta agctaatcag gacaataccg actcctgacc agatagcata agcaatccct
     6781 gtaggaatat aagccagcgt ctgagctaat aaccagaatg atgcacaata acaaataatt
     6841 gtaccaacag atggccataa ccgtgtaaaa ccttctgaaa acttcattaa ggttgtacca
     6901 atgacctctg caagtattgc accaccaaga taaatataag ggttcataga atattctttc
     6961 ctgttcaaac tggagagaat tgtactacag tttgaactca actcacctgt ttcatcattg
     7021 tgtacccatt gatgttcttt tatataccct caatacccgt ttcatcgcgg cactctggcg
     7081 acactcctta aaaatcagat tcgtgctcac ctttccttcc cgttcttctc tggtagcaaa
     7141 ccggtaatac accgttcgcc agaccttacc ttcgatagcc agaagacctg cccgtgccat
     7201 tttagccgca gcctgattta tgctggttac cgttgcgcct gttaccgcgg caacgtcctg
     7261 cgcacagaat ttcttatgag tccccaggta atgaataatt gcctctttgc ccgtcatacc
     7321 cttgctcctt tcagcccaaa cttagctttg atttctgcga tcttcgccag agcctgtgca
     7381 cgatttagag gtctaccgcc catgacagga agttgtttta ctggttcagg tatagcctca
     7441 ccacggttaa ttcgtgcggt catacaggac agttcatcgg cagccttgcg ccgtaattcc
     7501 gcgtcagtca acgcattggc ccgcatgttc tgatacaggt tggtaaccag ccagtagtgc
     7561 gcgtttgatt tccacggata agactctgca tccggataca gcccgcgctt ccggcaatac
     7621 tcgtaaacca tatcaaccag ctcgctggcg tttggcagcc cggcggtaac ggatgcttct
     7681 tcccggcacc aggcgacaaa ctgcccgggt gatggcagga atggtcgatt ctgccgacgg
     7741 gctacgcgca ttccagcgtt aacctgttcc attgtggtga tcccgttttc ccgaaaagcc
     7801 agcacccact ggcggcggat ttcgttcagt tcattctggt cacggttagc caggctcgcc
     7861 gggaaagttg ccagtaactg gctgaacaca ccgttgatga tctgcgctac ctgctgtacc
     7921 tgcggctttt cgtcgtactg ttccggcatg ttgttggcga tccgacgcat ctgctcacgg
     7981 tcaaagttaa ccatctgtgc ggcgatgttt ttcataaatc caccccgtaa atccagtcag
     8041 tgtttgtcag gtcgagtttt ggtttgctgg ctatcatgcc tgcctgttgc ttgttacggt
     8101 tgatttcgag ttgggtccac ttatcgcgga gtttggccgg gctcagcacg ttaccggacc
     8161 agaagttgtc ctggcatgcc cagcggaaca gcacgcacat gtcgcggtgg ttacgtccgt
     8221 cacgttcacg catcaggcgg atatcgttag cccacccagc aaaattcggt tttctggctg
     8281 atggtgcgat agtcttcacc atgtcaaaca tccactctgc ggcggtcagg tcttctgctg
     8341 tcccccactt gctgccgctc tgaattgcag catccggttt caccacagaa aggtcgtttt
     8401 ctggctggtc agaggattcg ccagaattct ctgacgaata atcttttctt ttttcttttg
     8461 taatagtgtc ttttgtgtcc ccctgttttg agggatagca atcccccaat ttgagggatg
     8521 ttttatccct cgttttaggg gattttccct cgttttgagg gatgcaccat tctgagatgt
     8581 ttttatttgg tccaaacatg ccgccttgct gcttgataat attcattctg acgagttcta
     8641 acttggcttc attgcaccgt ttgacgggta actttgtaat ctcgctaagt tgagaatcgg
     8701 tgattctgtc cattggttta ttccacccat aggttttacg cagaatggca agcagcactt
     8761 taaactgtcg cttggtcaga tctgcgcccg aataagcctc aagcagcata tttgatagtc
     8821 tggcgtaacc atcatcgaga tctgccacat tacgctcctg tccggcaaag ttacctctgc
     8881 cgaagttgag tatttttgct gtatttgtca taatgactcc tgttgataga tccagtaatg
     8941 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     9001 ggtgagaatc gcagcaactt gtcgcgccaa tcgagccatg tcgtcgtcaa cgaccccccc
     9061 attcaagaac agcaagcagc attgagaact ttggaatcca gtccctcttc cacctgctga
     9121 tctgcgactt atcaacgccc acagcttccg ctgtcttctc agttccaagc attgcgattt
     9181 tgttaagcaa cgcactctcg attcgtagag cctcgttgcg tttgtttgca cgaaccatat
     9241 gtaagtattt ccttagataa caattgattg aatgtatgca aataaatgca tacaccatag
     9301 gtgtggttta atttgatgcc ctttttcagg gctgggatgt gtaagagcgg gaatgtctta
     9361 agcggcttta ccgcgtttag ttccgtactg taaccaaacc ggatcacagt taagcgccat
     9421 agcaatctca aacaagaagc gcggtcgctt ggttactcca gcttcaatca gttgaattga
     9481 ttgctgttta acaccggctt tggttgccag ttcggtttgc gtcattttta acgcaattcg
     9541 cctcttcttg aggcgttcag aaagagtttg catatcgcct ccatcaacaa actttcttgt
     9601 attttcatac aatgtatctt gtttgtcaaa tacagttttt cttgtgaaga ttgggggggt
     9661 aaataacaga ggtggcttat gagtatttct tccagggtaa aaagcaaaag aattcagctt
     9721 ggacttaacc aagctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
     9781 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
     9841 gaaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
     9901 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
     9961 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
    10021 ttaggcgatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
    10081 agcatccctg aaggtcatat ggtgttagta gatactggac gcgagccagt gaatggaagc
    10141 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggttatagat
    10201 ggcgggcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacggg
    10261 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtatg atcaggattg
    10321 cggcgctact ctcaatactc ttaactacca gcgccaattc tgaatgctgg attgtcacaa
    10381 acctgcacgg gtacggggca atgaatggcg atcgttacga gtttacaaaa gacagcacgg
    10441 aagattccgt tttccacgta acaataaatg gcgataaatc atcagtttat gaatcagttt
    10501 ctggcgtcta tccagagatg aaatacactg ctttgtcatc gaacactatg gtaggagaat
    10561 accagtctgg aggaggaata accgttgaaa cctggtcaat cactacaaac aaaaaagctc
    10621 tttactccaa agtaatgaac atcccaggta tgcaacaact tacatcaacc aaatcctttg
    10681 ttggtgatgt agtcggaacc tgcaaccagt aatccccacc tcaatctcga taaccaaaaa
    10741 acaaactatt ttccgtttaa aaacaatgaa gtttgttttt catgcccctt ttttacaata
    10801 tttcttgttt acaacataca atctttcttg taattttaag ccatcagcag gacgcactga
    10861 ccaccattga aggtgatgct cttaaaaatt aagccctgaa gaagggcagc attcaaagca
    10921 gaaggctttg gggtgtgtga tacgaaacga agcattggcc ggaagtgcga atccggatta
    10981 gctgcaaatg agccaatcgt ggggtgtttt cgttcaggac tacgactcac acacaccacc
    11041 aaagctaact gacaagagaa tccagatgga tgcacaaaca cgccgccgcg aacgtcgcgc
    11101 agagaaacag gctcaatgga aagcagcaaa tcccctgttg gttggggtaa gcgcaaaacc
    11161 agttaaccgc cctattctct cgctgaatcg caaaccgaaa tcacgagtag aaagcgcact
    11221 gaatccgata gaccttacgg tgctggctga ataccacgaa cagattgaaa gcaacctgca
    11281 acgtattgag cgcaagaatc agcgcacatg gtacagcaag cctggcgaac gcggcataac
    11341 atgcagagga cgccagaaaa ttaaaggtaa atctatatca cttatttaga aaatgcagat
    11401 ttagggaaca gataggaggc gttacaccta tggcatctca tcctatggtt agaaggtggt
    11461 gcaaatcctt cgtattgaag tatggatttc acagaagatt catagcattg agcgcaaaga
    11521 tagtgcattg gctgaccggt atttgccgat tttttgagac gataaactac cgtagcaaca
    11581 gtaggtgtat acatctcata gtttttcttt tcctcttccc acttagaagc tcgatttatc
    11641 ttttcttcaa gctcaataat cttatcctta gaaatcatca aaagctcatt aagtgacatt
    11701 tgctgctgtt gggcatccat gagcttatcg acaagttcgt atgttttttc ttttactgag
    11761 tagtctattt gcattttctg gatttccttt actgcgccaa cagcactcat cagagcacct
    11821 ccggcaccag aaactgcatc tgtaatccta cttattattc ctttttcatc agacatataa
    11881 atcactctct tactgtaggg gtaagaggat tttactattt ttctcgctgt aggggtacac
    11941 gagaaccacc gagcctgatg tggttaaaag acaggcatac taataaacac tgcactgtgt
    12001 atttattcca acgagtgaat acacggagca atgtcgctcg taactaaaca ggagccgact
    12061 tgttctgatt attggaaatc ttctttgccc tccagtgtga gggcgatttt ttatctatga
    12121 ggatatgaat agatgtcaaa catcaaaaaa tacatcattg attacgactg gaaagcatca
    12181 atagaaattg aaatcgatca tgacgtaatg acagaggaaa aacttcacca gattaataat
    12241 ttctggtcag actctgaata ccgactcaat aaacacggct ctgtattaaa tgctgtatta
    12301 atcatgctgg cgcaacatgc tctgcttata gcaatttcaa gcgacttaaa tgcatatggt
    12361 gttgtgtgtg agttcgactg gaatgatgga aatggtcagg aaggatggcc tccaatggat
    12421 ggtagcgaag gaataagaat taccgatatc gatacatcag gaatatttga tccagatgat
    12481 atgactatca aagccgcctg agcgcggcgt taccgcatac caataacgct tcactcgagg
    12541 cgtttttcgt tatgtataaa taaggagcac accatgcaat atgccattgc agggtggcct
    12601 gttgctggct gcccttccga atctttactt gaacgaatca cccgtaaatt acgtgacgga
    12661 tggaaacgcc ttatcgacat acttaatcag ccaggagtcc caaaaaatgg atcaaacact
    12721 tatggctatc cagactaaat tcactatcgc cacttttatt ggcgatgaaa agatgtttcg
    12781 tgaggccgtc gacgcttata aaaaatggat attaatactg aaactgagat caagcaaaag
    12841 cattcactac cccccttccc tgttttccta atcagcccgg catttcgcgg acgatatttt
    12901 cacagctatt tcaggagttc agccatgaac gcttattaca ttcaggatcg tcttgaggct
    12961 cagagctggg cgcgtcacta ccagcagctc gcccgtgaag agaaagaggc agaactggca
    13021 gacgacatgg aaaaaagcct gccccagcac ctgtttgaat cgctatgcat cgatcatttg
    13081 caacgccacg gggccagcaa aaaagccatt acccgtgcgt ttgatgacga tgttgagttt
    13141 caggagcgca tggcagaaca catccggtac atggttgaaa ccattgctca ccaccaggtt
    13201 gatattgatt cagaggtata aaacggatga gtacagcact cgcaacgctg gctgggaagc
    13261 tggctgaacg tgtcggcatg gattctgtcg acccacagga actgatcacc actcttcgcc
    13321 agacggcatt taaaggtgat gccagcgatg cgcagttcat cgcattgttg atcgtcgcca
    13381 accagtacgg ccttaatccg tggacgaaag aaatttacgc cttccctgac aagcagaacg
    13441 gcatcgttcc ggtggtgggc gttgatggct ggtcccgtat catcaatgaa aaccagcagt
    13501 ttgatggcat ggactttgag caggacaatg aatcatgtac atgccggatt taccgcaagg
    13561 accgtaatca tccgatctgc gttaccgagt ggatggatga atgccgccgc gaaccattca
    13621 aaacccgcga aggcagagaa atcacggggc cgtggcagtc gcatcccaaa cggatgttac
    13681 ggcataaagc catgattcag tgtgcccgtc tcgccttcgg atttgctggt atctatgaca
    13741 aggatgaagc cgagcgcatt gtcgaaaata ccgcatacac tgcagaacgt cagccggaac
    13801 gcgacatcac tccggttaac gatgaaacca tgcaggagat taacactctg ctgatcgccc
    13861 tggataaaac atgggatgac gacttattgc cgctctgttc ccagatattt cgccgcgaca
    13921 ttcgcgcatc gtcagaactg acacaggccg aagcagtgaa agctcttgga ttcctgaaac
    13981 agaaagccac tgagcagaag gtggcagcat gacaccggac attatcctgc agcgtaccgg
    14041 gatcgacgtg agagctgtcg aacaggggga tgatgcatgg cacaaattac ggctcggcgt
    14101 catcaccgct tcagaagttc acaacgtgat agcaaagccc cgctcaggaa agaagtggcc
    14161 tgacatgaaa atgtcctact tccacaccct gctggctgag gtttgcaccg gtgtggctcc
    14221 ggaagttaat gctaaggcgc tggcctgggg aaaacagtac gagaacgacg ccagaaccct
    14281 gtttgaattc acttccggcg tgaatattac tgaatccccg atcatctatc gcgacgaaaa
    14341 tatgcgcacc gcctgctctc ccgatggttt atgcagtgac ggcaacggcc ttgaactgaa
    14401 atgcccgttt acctcccggg atttcatgaa attccggctc ggtggtttcg aggcaataaa
    14461 atcggcttac atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta
    14521 ctttgccaac tatgacccgc gcatgaagcg tgaaggcctg cattatgtcg tgattgagcg
    14581 gaatgaaaag tacatggcga attttgacga gatggtgccg gagttcatcg aaaaaatgga
    14641 cgtggcactg gctgaaattg gttttgtatt tggggagcaa tggcgatgaa gcatcctcac
    14701 gataatatcc gggtaggcac gatcactttc gtctactccg ttacgaagcg aggctgggta
    14761 tttcccggcc tttctgttat ccgaaatccc ctgaaagcac agcggctggc tgaggagata
    14821 aataataaac ggggagctgt atgcacaaag catctcccgt tgagttaaga acgagtatcg
    14881 agatggcaca tagcctcgct caaattggag tcaggtttgt gccaatacca gtagaaacag
    14941 acgaagaatt tcatacgtta gccgcatccc tttcacaaaa gctggaaatg atggtggcga
    15001 aagcagaagc agatgagaga gaccaggtat gacaaccacg gaatgcattt ttctggcagc
    15061 gggcttcata ttctgtgtgc ttatgcttgc cgacatggga cttgttcaat gacacctcag
    15121 caggaaaacg cccttcgcag cattgcccgt caggctaatt ctgaaatcaa aaaagccaga
    15181 cagcagtttc cggataaaaa cgtcgatgac atttgccgta gcgtactgaa gaagcaccgc
    15241 gaaacggtaa cgctgatggg attcacaccg actcatttaa gcctggcaat cggcatgtta
    15301 aacggcgtct ttaaggaacg atgaacatga aaagcaaaat catcagggag ctacaggctc
    15361 cttttttatt attcgcattc accctcaagc gtattaacca acaattcagg gattaatgga
    15421 agatggcaga catcattgat tcagcatcag aaattgaaga attacagcgc aacacagcaa
    15481 taaaaatgcg ccgcctgaac caccaggcta tatctgccac tcattgttgt gagtgtggcg
    15541 atccgataga tgaacgaaga cgcctggtcg ttcagggttg tcggacttgt gcaagttgcc
    15601 aggaggatct ggagcttatc agtaaacaga gaggttcgaa gtgagcgaaa ttaactagaa
    15661 gccaaagata aaatcatcgc tgagcaggag aaaatcgcta acggagaaaa gacagtaagt
    15721 cagtatatga aaaccgcatg atatcatcag ataaaaatcg gtcgtaaagc gaaatattaa
    15781 taccagaaca aacgagtcga ggtaaattat attacctcga taaattaact aaaa
//
LOCUS       DAMZII010000105        15402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZII010000105 DAMZII010000000
VERSION     DAMZII010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.105"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            349..762
                     /locus_tag="POA77_003432"
     CDS             349..762
                     /locus_tag="POA77_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366808.1"
                     /translation="MLANISTKSDIQPPYITGLLDNVERALDKINILIDKERVYDGET
                     LAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPVDDIKNVMM
                     LYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
     gene            1972..2535
                     /locus_tag="POA77_003433"
     CDS             1972..2535
                     /locus_tag="POA77_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDI6366809.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            2690..3578
                     /locus_tag="POA77_003434"
                     /pseudo
     CDS             2690..3578
                     /locus_tag="POA77_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(3559..3924)
                     /gene="tnpB"
                     /locus_tag="POA77_003435"
     CDS             complement(3559..3924)
                     /gene="tnpB"
                     /locus_tag="POA77_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6366810.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSTRDGKVFLMPAQLAMLMEGIDW
                     RQPKRLLTSLTCCSPGLLR"
     gene            complement(3921..4236)
                     /locus_tag="POA77_003436"
                     /pseudo
     CDS             complement(3921..4236)
                     /locus_tag="POA77_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446914.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4280..5899)
                     /locus_tag="POA77_003437"
     CDS             complement(4280..5899)
                     /locus_tag="POA77_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6366811.1"
                     /translation="MGNAMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKTAQCP
                     ECSKRSDSVHSSRRRRIQHLPCSGQTLWLVFSVRHWYCRNPVCSRKIFAESLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPGTKQSGAPHV
                     GIDEWAWHRGHRYGKLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQVVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSLKKSPEPELSV
                     PAVSLRRPERLKQQTRKKRHQRWTEVMALHNKGCSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESGNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWIPPVTTAARLPSVSRVSRWLMPWRIIRGEENYASRFISLMCEKEPELKIAQQLV
                     LEFYRILKTQNKSQLSSWFTRVHESGSAELRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            complement(5925..6077)
                     /locus_tag="POA77_003438"
     CDS             complement(5925..6077)
                     /locus_tag="POA77_003438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366812.1"
                     /translation="MRTKERLPRKRYSPEFKMELVRLALEQDVTNGAIVVLPQLGWKK
                     MDETFW"
     gene            6161..6564
                     /locus_tag="POA77_003439"
                     /pseudo
     CDS             6161..6564
                     /locus_tag="POA77_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            7535..8300
                     /locus_tag="POA77_003440"
                     /pseudo
     CDS             7535..8300
                     /locus_tag="POA77_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            8300..9040
                     /gene="rfbB"
                     /locus_tag="POA77_003441"
     CDS             8300..9040
                     /gene="rfbB"
                     /locus_tag="POA77_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export ABC transporter ATP-binding
                     protein RfbB"
                     /protein_id="HDI6366813.1"
                     /translation="MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYL
                     AIEDITFTVGKGEAVALIGRNGAGKSTSLGLVAGVIKPTKGTVTTQGRVASMLELGGG
                     FHPELTGRENIYLNATLLGLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLG
                     FSVISQVEPDILIIDEVLAVGDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICD
                     RVVWIENHKLREIGSAERIIELYKQAMA"
     gene            9055..10947
                     /locus_tag="POA77_003442"
     CDS             9055..10947
                     /locus_tag="POA77_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HDI6366814.1"
                     /translation="MNSIKIYTCHHKPSAFLNASIIKPLHVGKANTYNDIGCEGDDSG
                     DNISFKNPFYCELTAHYWVWKNESLADYVGFMHYRRHLNFAEQQNHPEDNWGVVNYPL
                     INAEYESQFGLSDESISTCVDGYDLLLPKKWSVTSAGSKNNLDHYAKGEFLHIKDYQS
                     ALDVVEELYPQYKAAIQQFNNATDGYYTNMFVMRKDMFLDYSEWLFAILSNLEDRISM
                     NNYNAQEKRVIGHIAERLFNIYIIKCQQDKQLKIKELQRTFVTAETFNGKLKPVFDES
                     VPVVISFDNNYALSGGALINSIVLHSDASRNYDIVVLENKVSHLNKQRLIKLVAGHNN
                     ISLRFFDVNSFTEMSDVHTRAHFSASTYARLFIPQLFREYKKVVFIDSDTVVKADLAT
                     LLDVEIGTNLVAAVKDIVMEGFVKFGTMSESDDGIMPAEQYLKKTLGMTNPDEYFQAG
                     IIVFNVEQMVTENTFAQLMSALKAKKYWFLDQDIMNKVFFGRVKFLPLEWNVYHGNGN
                     TDDFFPNLKFSTYMRFLQARRNPKMIHYAGENKPWNTEKVDFYDDFLENVLSTPWEKE
                     IYYRQLPVATVVPNQHTELQQTVLLQTKIKRALMPYVNKYAPVGSPRRNKLIKYYYKV
                     RRSILG"
     gene            10966..12120
                     /gene="glf"
                     /locus_tag="POA77_003443"
     CDS             10966..12120
                     /gene="glf"
                     /locus_tag="POA77_003443"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023284018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HDI6366815.1"
                     /translation="MKRNNILIVGAGFSGVVIARQLAEQGHKVKIIDQRDHIGGNSYD
                     TRDPQTDVMVHVYGPHIFHTDNETVWNYVNQYAEMVPYVNRVKATVNGQVFSLPINLH
                     TINQFFAKTCSPDEARALISEKGDSSILEPKTFEEQALRFIGKELYEAFFKGYTIKQW
                     GMQPSELPASILKRLPVRFNYDDNYFNHKFQGMPKLGYTHMIEAIADHENITLQLQRE
                     FAAENRESYDHVFYSGPLDAFYSYQHGRLGYRTLDFERFTWQGDYQGCAVMNYCSVDV
                     PYTRITEHKYFSPWENHEGSVCYKEYSRACGENDIPYYPIRQMGEMALLDKYLSLAEG
                     EKNITFVGRLGTYRYLDMDVTIAEALKTADKYLSSLSNDETMPVFVADVR"
     gene            12117..13007
                     /locus_tag="POA77_003444"
     CDS             12117..13007
                     /locus_tag="POA77_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6366816.1"
                     /translation="MKHTALIVTFNRLEKLKKTVAETVKLHFTSIVIVNNGSTDGTSD
                     WLQTITDPRVIVLNLASNNGGAGGFKAGSQYICSSVDSDWVFFYDDDAYPQSDILDKF
                     ATINKEDCRVFTALVKDLQGRTCAMNVPFVKVPTSFSDTLQYIKHPQRYVPDNKKMLV
                     QTVSFVGMIIKREVLNEHLHHIYDELFLYFDDLYFGYQLTLSGEKITYQPELVFTHDV
                     SIQGKVISPEWKVYYLCRNLILAKRIFTEVEIFSSSSILLRLCKYITIFPVQRRKWVY
                     LKFLCRGIVHGVKGISGKFH"
     gene            13008..14153
                     /locus_tag="POA77_003445"
     CDS             13008..14153
                     /locus_tag="POA77_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6366817.1"
                     /translation="MDIAMKKLCYFINSDWYFDLHWTDRAIAARDAGYEIHIISHFVD
                     DKIAEKFRTLGFVCHNIPLVAQSFNVLIFFRAFSKARKIIQNINPDLLHCITIKPCLI
                     GGFLAKSTHRPVILSFVGLGRVFSAESACLKLLRSFTVMAYKYIASNKCSLFMFEHDK
                     DRAKLADLVGIDYKQTIVIDGAGINPEIYKYSLEQQRDVPVVLFASRMLWSKGLGDLI
                     EAKKILSNKNIHFTLNVAGILVENDKDAIPLATIQKWQSEGVINWLGHCSNVFDLIEE
                     SNIVALPSVYAEGVPRILLEASSVGRACIAYDVGGCDSLIINNYNGLIVKSKSVEELA
                     EKLGFLLDNPETRVAMGINGRKRIQDKFSSVMIINKTLKTYRDVVEE"
     gene            14275..>15402
                     /locus_tag="POA77_003446"
     CDS             14275..>15402
                     /locus_tag="POA77_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6366818.1"
                     /translation="MSEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVND
                     CSDDNTLEIVSALAETDSRIRVINNTTNLGGGGSRNIGLDAATGEYVIFLDDDDYVDN
                     MMLERMYARASDIQADVVICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSD
                     FFRTFVWWPWDKLLRRQFITSHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISH
                     TINRSESLSATRAESHRCAVEALVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTI
                     SGPAFHPFYQQVKEFVVALDAKSDDFYDEFIAAAHHRITTLSAEEYLFSLKDRVLKEL
                     EFFQARSSALQQEVETLTHSLAGQKDENAILHNQLHEIEERV"
BASE COUNT         4122 a         3152 c         3601 g         4527 t
ORIGIN      
        1 cgtggttatg tgaccaggtg ctacatgtta ctggccgtcc ttatttatct gattcggcgg
       61 aacaaactcc acaaagctct tcggttttat gatgaatccc caaaatacga gttaccttct
      121 gggtatcttc ccggcgcttt ttcagttctc aggatagcgc tggagatcaa attaaacagg
      181 gagaaaataa agcatggttc actgttcgaa ttacttgatt atgtaaaagc ttatcaggat
      241 gcatttatgg acttaagggt cgtgacggat cctgcgtata atgaagatga aatacaatat
      301 gatgcgaata attttactct gatgcgtgtc attaaaatgt ataattcgat gcttgcaaat
      361 attagtacaa agtcagatat tcagcctccg tatattacag gtcttcttga taatgtagag
      421 agggcgttag ataagataaa tattctgata gataaagaac gtgtttacga tggtgaaacg
      481 ttggctgaat taattacaga gaacaaaata ttatccagtc gtgaaagtaa agaaaacctg
      541 ataggattat ttacgggcag acacaaatat actttactgc aatgtattga gaagcttggc
      601 gtattggttg attatgtaat atcaccggtt gatgatatta aaaacgtcat gatgttgtat
      661 ggaaataatg ctgaaaacaa gaacagacgc agactgatct acaacgcatt aaccatcatt
      721 tgtggtgatg atactaaaaa caaccagtca gatcctcgat gatattatcg gaacgtgttt
      781 ttggcatccg atgtttgtat atccctgcta ttgttgctag ccggctagca acaatagcag
      841 agggggcatg catgtctgaa aaaataaaaa atcgcaacgc attgattttt atgttgttta
      901 tatttcgcaa atactatctc tcgtttttgt gattagtgtc atgttttatc gtctcgcgga
      961 taaccgagag gagtgaacag taaccctgat tatatgatga attgtttctg tattgtatgt
     1021 gtgaaatatg cttctggtga tatgaaaaca tatccataac gtggtgtttc acgctgtatt
     1081 tttggggctg atattttatt ttgcgggttt tatttttttg tttgtgcaga agtacacatt
     1141 gaacatccaa tacaaaagtg tatgacctga agaggtctgg agctatgttg atttcctgtt
     1201 caaaaaagaa ttaaggttaa ttagtattat tagtgggagt tttgttatgt tcttattttg
     1261 ctatcttatg tctttatgtt gtaatgtctg ctcgccactg tatgctaact gtacacttta
     1321 ctctgtaagt atgtgtacag cattatctcc atactactgg tgtgagagtt tacctgtgtg
     1381 gtgaattata ctctttgaga gtaccataaa tgttgggaat gttaatcctg agtatgtgtc
     1441 tggtgcatag ttagtgttaa gtgtgtacca aaacgctatt agtgtgttat tagtctattg
     1501 aggtgcggtg gtattaataa atattatctt tttgtatggg gtaatcatct ctggtgtggg
     1561 cgtatctctc atatcgttat tctttatgct actggtcaaa tattggtgag acttcctcac
     1621 agagtttctg tttttaatta atggcaggat aatattaata gcgatgttac ctgaggccac
     1681 ctggtactga cttttgtgaa tgtcaggttg agtgaatggt gcttccttaa actcaattct
     1741 atgaggtgtg tttttaccat tgtaattctt actggtcctc tgtttaagag agcctcactg
     1801 aattctgaag tattacctcg ttacagcgat gcggtaatac aaaacagttt tatcagggtg
     1861 tctgtgctgc tggtttactt cgtttattaa tgccgtgcta cggcgtttat gttctctcag
     1921 tatttacata atttcctctt cctgtgagtt ttataaatta aggactataa aatgtataat
     1981 gccaatccca attacgaaat gaatttcgcg attctgaaag atgtgaatga acacatggag
     2041 ggattgtttc aacgtttttc taaactgtta cctttcagaa ttgattttgc ctacaggaaa
     2101 gacacgccca gtttcgggca ttcctgtaag cacagtatgt gtatggaaat ataccgttta
     2161 cttagcgaaa cccagacgat gttggcagga tattactggg ttatggaata tacaccagac
     2221 aaagggcttc atattcattt tatcggctat ctggatgggc aacgtcacaa gaactcttat
     2281 cagatatcca ggcaactggg ggatatctgg aggcggatca cggaagggga gggttatttt
     2341 catctgtgcc gggctaaaga taaatacccg gtgcgtatcg atcacgtgat tcactatagt
     2401 gataagtccg cagtagacga cttacgttac gccctcagct acctggctaa acaggatcag
     2461 aaagaacacg gtattattct gggccgtagc aggcttcccg aaaaaagtaa ccggggccga
     2521 ccacgacaca actgaattac agctgtatta ggtattagaa ttaagtttct gtaagttgtt
     2581 gggaggtgct tgagttaata ggcagaagag ggcggggcgc tttgtcatca gtgtagggga
     2641 cccgaatttc tgccgtggtg cctcagtgga tcggatacag gtaacgaata tggttcataa
     2701 atctgacagc gatgaattag ctgcattgag ggcagaaaat gcccgtctgg tctcattact
     2761 tggaatggcg acgtaaaccg ccgagccctg tgcagcgcgt ttccgtatta tccaccgacg
     2821 agaaggttgc attatttcgt cggttgtttc gtgggcgtga tgatatatgg gcccttcgtt
     2881 gggaaagtaa aaccagcggc aaatcagggt actctcctgc ctgcgctaac gaatggcagg
     2941 cgagaatatg cggtaaaccc cggataaaat gcggggactg tacgcaccgt cagttactcc
     3001 ctgtatctga tcttgtcatc taccagcatc tggcaggcac gcatactgcc gggttatatc
     3061 cgttgctgga agatgattcc tgttattttc tggctgttga ttttgataaa gctgaatggc
     3121 agaaggatgc atccgcgttc atgcgagcct gtgatgagct gagtgtacct gctgcgctgg
     3181 aaatatcccg ctcacgtcag ggagcgcatg tctggatatt ttttgcttca cgagtttcgg
     3241 cccgcgaagc tcgccgtctg gggactgcta ttattagcta tacgtgcagc cggacccgac
     3301 agctgcaatt gagttcttat gaccgattat ttcctaatca ggatactatg ccaaaaggag
     3361 gatttggtaa tcttatcgcg ttacctctgc aaaaaagacc gcgtgcatcg gggggaagcg
     3421 tttttgttga tattaatttc cagccttatt ctgatcaatg ggcttttctt gcatcggtga
     3481 tcccgatgaa tgtgcaggat attgaatcga cgatattacg ggctacagga agtatccatc
     3541 ctctggatgt gaactttatc aacgaagaag acctgggcta caacatgtca gggacgtcag
     3601 taaccgcttt ggctgtcgcc agtcgatacc ttccatcagc atcgccagct gcgccggcat
     3661 caggaacact ttgccatcgc gggtcgacgg ccaggcgaag cggccacgct ccagcctttt
     3721 ggtcagcagg cacagtccgt cgccggtgga ccacagcagt ttgacctgac tgccgctgcg
     3781 gccccggaag atgaagacgt ggccggacat cgggtcatct ttcaacgctg tctgcacctt
     3841 tgcggcgaga ccattgaagc cgttgcgcat atcggtgata ccggcaacca gccagatttt
     3901 tgtgcctgcg ggaagattta tcatacagac ccccgcatca gttcgcgcag gacagtgctc
     3961 ataagctcaa ctgagggaga ggtcagcgtt atttctccgc cccgacattt tatgtggcag
     4021 gcagtgggag gtgaacagga tagtggttcg aattccggga gagaaggctc cgcctgaagt
     4081 tgcacgggta tcagggtagg aagcgacgat gcattttttc ggggtcgaca gacccgcccg
     4141 cccttcacgt tgctagagcc ttatccattt aaagagcaga ttatcgttga cgtcatgttg
     4201 acgggccaga gcagcgatac tgccctcttg tgtcaacgac ggatgaaaag tggttcttcc
     4261 gcaacttggg tggagatttt catgccagtg gactcatgac cctctgcctg agcagttcaa
     4321 atccggctcg accatacatc tggcgtttca acatcttcag gcgatttaca tgaccttcga
     4381 caacaccatt actccagcga ctgctgattg cctcacatat agccgcagca tcagcttcca
     4441 tccccgcagc cacgcgccga agttctgctg agccgctttc gtggactcga gtgaaccagc
     4501 tgctaagctg tgatttattt tgggttttca gaatacggta gaactcgagt accagttgct
     4561 gcgctatttt cagctccggt tctttttcac acatcagact aataaatcgg gaagcataat
     4621 tttcttcccc cctgattatt ctccagggca tcaaccaccg gcttacccgg gacactgaag
     4681 gaagtctggc ggcagtagta accggtggga tccagccttt acgccatttg gcaacagcat
     4741 ccctgacgat ggtttcactg cctgtaaacc cccgggccac catttcccgc catatccggc
     4801 tggcgttata attaccgctt tctcgttgct cttttaacca ctccctccat gggtccagaa
     4861 gccctcgctt tggaggtcgg gttgacattt cagggaatgt tcctgaacgc acccagcgac
     4921 tgactgtcac acgcgacagg cctgtaatac gggatatttc cctgaaacta catcccttgt
     4981 tatgcagggc cataacctct gtccaacgct gatgccgttt tttgcgggtt tgctgtttaa
     5041 ggcgttccgg acgacggagc gatactgcag gcacagacag ttctggctca ggtgatttct
     5101 ttagtgacaa ctcactggca acaagacgta tcagaggtat atgtctgtac atcattcgtt
     5161 caagcgcatc gccaatattt tttagcaggt gccatcgatc ggccacctgt ctggcctgag
     5221 gtgcaccttc acgtgctgct gttgcataga ctccactgcg atcacgcgag acaacctgta
     5281 tttccggata ttttctgaac caggtcgcca gcgtacgctg atcacgaccg ggaagcagga
     5341 cgaggggacg gtgagtatca agattgacga ttaacttacc gtaacggtgg ccccgatgcc
     5401 acgcccactc atcaataccg acatgaggcg cgcctgactg tttcgtcccc ggggtattga
     5461 taaccctgcg aagaagagta tctgcactgc aacggagacc cactgccgtt gcagcccgtt
     5521 ttccagcctc acctccggct attaatccca gttgacgttg taaattttgt aacgcctgtg
     5581 aagactgctg gtgtgaaccg gcgaagggag caagcgactc ggcaaaaatt tttcgtgaac
     5641 aaacagggtt acggcagtac cagtggcgga cggaaaatac aagccatagc gtctgcccgg
     5701 agcagggtag atgctgtatc cggcgccgac gagaactatg aacagagtcg ctacgcttag
     5761 agcattcagg acattgtgct gttttgcgtt ttccgtggag atggagggtg ataccgtcag
     5821 agctaataat ttgtcgactg catcgccatc cgcaaggtaa ctgtagaagt gtcttaagag
     5881 agtgcattgc gttgcccata acttaaaaca gcttagatcc ttgttcacca gaaagtttca
     5941 tccattttct tccacccaag ttgcggaaga accactattg caccgttcgt gacatcttgt
     6001 tcaagggcca gcctgaccag ttccatttta aattcaggag aatagcgttt ccgggggagt
     6061 cgttctttgg tgcgcatgag gtgtccacct gcaaaataag tgggtgctta agttacagga
     6121 tcctggattc gggaccagac ggtactcaga cgacgcttac agaaaaccag tgggctgggc
     6181 catgtcgttc tcgccggaca gcaggctcac catgaaagcg ctggaaatgg cataggaaac
     6241 ccgtggtaag cccggtgggg tgatgttcca cagcgacagg gcagtcatta tacgagcagg
     6301 cagttccagc agttattgtg gcgataccag atcaggcaga gtatgagccg gcgcggaaac
     6361 tgctgggata acagcccaat ggaacgcttc ttcaggagtc tgaagaacga atggatgccg
     6421 gtggtgggtt acgtaagctt cagcgaggca gctcacgcca taacggacta tatcgttgga
     6481 tattacagcg cactaagacc gcacgaatat aacggtgggt tacccccaaa cgaatcagaa
     6541 aatcgatact ggaaaaaact ctaactcggt ggccagtttt tgttgaccac ttcagtattt
     6601 tttggctgga tggattggtc atgctttttc atttttattc ttccagacca gagctataat
     6661 gcgcgcgtaa ttcacgtcct acggatgcat tattgataat gcgcagcaaa cgtaccctac
     6721 gacgctcgga tttgaccact tttgaagaaa agtcaaattc acttttatgc aagataggat
     6781 gaggaagtac gttatcctgg tggtgttgcc gctgcctgcc cgcgaactta atactctgcc
     6841 tgactcatgt agagatgcgg agcgcccagc tacatggctg ttgaagtcat actgccgaga
     6901 gtgacgcttt ttgttggttt caagggtgaa atgagaaagt cctgatggat aggatgccat
     6961 aaactgggga aagtagcggt gcatttgttg cggtgtagcg atggatgtga gtccggagag
     7021 gggcgctgag tacagatatc cgtttgtttt tagctttttt gccgaacgct gactaggctg
     7081 tttgtggctt catgaatgct gcgccggtaa tgcttgtgga gctaacgacg agcgtatcct
     7141 cttcgttacg gtaagaccgg gtaagacaga cactttagag aggggagtcc gcattttggt
     7201 tgcgatagcc aatggcggta gcgtgtctga attttagaat aaactcattg ttcgatttaa
     7261 cattttattt acaatcttaa aggatggttt atgtatcttc cacgagaatc acattttcat
     7321 tcagcggtta ctgaggttag cgatcatctc cgattccgga gtgctaatgc gcaggaaaga
     7381 ttgtttacat attgcttgtg caaaatgatg ttacaattgg tctttgttta tgggaatagc
     7441 tgttcgtctc tcgagcacag cagatcttat cgataattct gtttgataca tattacgtac
     7501 ggaatttttt gtagaataat ttatgagtat aaaaatgaag tataatatag ggtatttgtt
     7561 tgatcttctt gttgttataa caaacaaaga tttaaaggta cgttataaaa gcagcgtatt
     7621 tggttactta tggtcaattg cgaatccact gctttttgca atgatatatt atttcatttt
     7681 taagctcgtt atgcgagtgc agatcccgaa ttataccctt ttcttgatta ccggtttatt
     7741 tccgtggcaa tggtttgcca gctcggcgac aaattccttg ttttcgttta tagccaatgc
     7801 gcaaataatc aaaaaaacag tttttccgcg ctcagttatt ccattgagta atgtcatgat
     7861 ggaaggggta catttcttgt gtacaattcc tgtgattatt gcattcctgt tcgtgtatgg
     7921 tatgcgtcca tcgctctcat ggctctgggg cgtaccaatt attgcgcttg gtcaggtgat
     7981 ttttacattt ggtatttcaa tcatattttc tacgctaaat ctgttcttca gggatttaga
     8041 gcgatttgtc agccttggta tcatgctcat gttttattgc acgccgatat tatatgcgtc
     8101 agatatgatt cctgaaaaat ttagctggat tattacctat aatcctctgg caagcatgat
     8161 cctcagctgg cggcagctgt tcatggatgg cgtcttaaat tatgaatata tttcaatcct
     8221 ttacattaca ggtttagtcc tgacaattgt gggcctgagt atttaataaa ttaaaatatc
     8281 gatttgcaga gattttgtaa tggaaccagt aataaacttt agtcacgtta cgaaagagta
     8341 tcctctttac caccatattg gctcaggaat taaggatctc gtatttcacc ctaaaagagc
     8401 ttttcagctg ttaaaagggc gtaaatatct tgcgatcgaa gacattacct tcaccgtggg
     8461 taaaggcgag gccgtagcgc tgattggtcg taacggcgca gggaaaagta cctccctcgg
     8521 gctggtggcc ggcgtcatta agcctacaaa aggcactgtt acaacccagg gacgcgttgc
     8581 ctcaatgctt gagctaggcg gcggttttca ccctgaatta accgggcgag aaaatattta
     8641 tctcaatgcg accctgcttg ggctacgccg caaagaggtt cagcaacgga tggatcggat
     8701 aattgagttc tccgagctgg gtgatttcat tgacgaacct attcgcgtgt actcaagtgg
     8761 tatgcttgcg aagcttggat tctctgtcat cagccaggta gaacccgata tacttattat
     8821 cgatgaggta cttgctgtcg gtgacatttc gttccaggcc aaatgtatac aaacaatacg
     8881 agacttcaaa aataagggtg taacgatatt attcgttagc cacaatatga gtgatgttga
     8941 aaaaatctgt gacagagttg tttggatcga aaaccataaa ctccgcgaaa ttggctcagc
     9001 agagagaatc attgaacttt acaagcaagc aatggcttaa taattggtaa taagatgaat
     9061 agtatcaaaa tttatacatg tcaccacaaa cccagtgctt ttctaaatgc ctctattatt
     9121 aaaccactgc atgtcgggaa ggctaatact tataatgata ttggctgtga aggggatgat
     9181 agcggagaca acatttcctt taaaaacccc ttctattgtg agctgacggc ccactactgg
     9241 gtgtggaaga atgaatctct ggctgactat gtgggattca tgcattaccg cagacacttg
     9301 aactttgcag aacaacaaaa tcatcctgag gataactggg gggtagtcaa ctacccgctc
     9361 atcaatgccg aatacgaaag ccagtttgga ttaagtgatg aatccataag cacctgcgtc
     9421 gatggatatg atctgctgtt acccaaaaaa tggtcggtaa catcggcagg aagtaagaat
     9481 aaccttgatc attatgcaaa aggtgagttt ttacacatta aagactatca gtctgccctg
     9541 gatgtcgttg aagagctgta tccacaatat aaagctgcga ttcaacaatt caataatgca
     9601 actgatggtt attatacgaa catgtttgtt atgcgcaaag acatgttcct ggattattct
     9661 gaatggcttt ttgctatttt gtctaatctt gaagatcgga tttcgatgaa taactacaac
     9721 gctcaagaaa aacgagttat aggacacatt gctgagcgtt tattcaatat ctatatcatc
     9781 aaatgtcaac aagataaaca gctaaaaata aaagaactac agcgcacgtt tgttactgct
     9841 gaaacattta atggtaaatt aaagcccgtt tttgacgaaa gcgtaccggt tgttattagt
     9901 tttgataata actatgcatt aagtggcggg gcattaatca attcaattgt tctccattct
     9961 gatgcgagca gaaactatga tatcgttgtt ctggaaaata aggtcagtca tttaaataaa
    10021 caacgcctta tcaagctagt tgctggtcat aacaacatat cattgcgctt ttttgatgtg
    10081 aattcattca ctgagatgag tgatgttcat acccgtgcac attttagtgc gtcgacctat
    10141 gcgcgcttgt tcatcccgca acttttccgc gagtataaaa aagttgtgtt tatcgattct
    10201 gacaccgtgg tgaaggctga tttggcgaca cttctggatg tcgagatcgg cactaacctg
    10261 gttgccgctg ttaaagacat cgtcatggaa gggtttgtga agtttggtac catgtcagaa
    10321 tcagatgatg gcattatgcc tgcagagcaa tacctgaaaa agacattagg aatgactaat
    10381 cctgacgaat attttcaggc cgggattatt gtttttaatg tcgaacaaat ggttacagag
    10441 aatacctttg ctcaattgat gtcagcattg aaagccaaaa agtattggtt cttagatcag
    10501 gatatcatga acaaagtctt ctttggccga gtcaaattct taccattaga atggaacgtg
    10561 taccatggta atggtaatac cgatgatttc ttcccgaatc tcaagttttc aacctatatg
    10621 cgctttttgc aagccagaag aaatccaaaa atgattcact atgcgggtga aaacaaacca
    10681 tggaatactg agaaagtcga tttctatgat gattttcttg agaatgtttt aagtacgcca
    10741 tgggagaaag aaatctacta tcgccagtta cctgtggcca cggtagtacc taaccaacat
    10801 actgaactgc agcaaaccgt gttactgcag acaaagatta aaagagcttt aatgccatat
    10861 gttaacaaat atgctcctgt cggttcgcca agaagaaata agctcatcaa atattattat
    10921 aaagttcgcc gttcgattct tggctaatac aactggagat acattatgaa gcgtaacaat
    10981 attctcatcg tcggcgctgg tttttcaggt gtggtcattg cccgccagct tgctgaacaa
    11041 ggtcacaagg ttaaaatcat cgatcagcgt gaccacattg gaggaaactc ttatgacact
    11101 cgcgatcctc agactgatgt catggttcat gtctacggtc cgcatatttt ccatacggat
    11161 aatgaaaccg tctggaacta tgtcaatcag tatgcggaaa tggtgcccta tgtgaataga
    11221 gtaaaagcta ctgttaatgg tcaggttttt tcactgccga ttaatctgca tactattaat
    11281 cagttctttg ctaaaacctg ttctcctgat gaagctcgcg cgctcattag cgaaaaaggt
    11341 gatagttcca ttctggaacc gaagactttt gaagagcagg ccttgcgctt cattggtaaa
    11401 gaattatacg aagcgttctt caaaggctac accataaaac agtgggggat gcagccttct
    11461 gagcttccgg catctatact caaacgtttg cctgtgcgtt tcaattatga cgataattat
    11521 ttcaatcata aattccaggg catgccgaag ttaggctata cccatatgat tgaagcgatc
    11581 gccgatcatg aaaatatcac tctgcaatta cagcgtgagt ttgcggctga aaatcgcgaa
    11641 agttatgatc atgtatttta tagcgggccg ttagacgcct tctattcata ccagcatggg
    11701 cgtcttggct accgtactct ggactttgag cgatttacct ggcaaggtga ttaccagggc
    11761 tgcgcggtca tgaactattg ctcagttgat gtcccgtata cgcgcattac cgaacataaa
    11821 tatttttccc cgtgggaaaa ccacgaaggc tccgtatgtt acaaagaata tagtcgcgcc
    11881 tgcggtgaaa atgatattcc ttactatcca atccgccaga tgggcgagat ggctctgctg
    11941 gataaatatc tatcactggc ggaaggtgag aaaaatatta ctttcgtcgg acgcttaggg
    12001 acttatcgct atcttgatat ggatgtaacg attgcagaag ctttaaaaac ggccgataaa
    12061 tacctgtctt cattgtccaa cgacgaaaca atgcctgtat ttgtggccga tgtacgatga
    12121 aacatactgc actaatagtc actttcaacc gtcttgagaa gctaaaaaag acggttgctg
    12181 aaactgttaa gctgcacttt acctctatcg ttattgtcaa taatgggtcc acggatggga
    12241 cctctgactg gctgcagaca atcactgatc cccgagtcat tgtcttaaat ctggccagta
    12301 ataacggggg ggccggcggg tttaaggccg gtagtcaata tatatgcagc tctgttgata
    12361 gcgattgggt cttcttttat gacgatgatg cttatccgca aagtgatatt cttgataagt
    12421 tcgcgacgat aaataaagaa gattgtcgtg tttttacggc tttagttaaa gatttacagg
    12481 gccggacatg cgcgatgaat gttccatttg taaaagtccc gacctcgttt agtgatacct
    12541 tgcaatatat caaacatccc caacgatatg taccagataa taagaaaatg ctggtgcaaa
    12601 ccgtttcatt tgttggcatg atcattaagc gtgaagtctt gaatgagcac cttcatcata
    12661 tttatgatga acttttcctt tattttgatg atctctattt tggttatcaa ttaactttaa
    12721 gtggcgagaa aatcacgtat caaccggagc ttgtttttac tcatgacgtg agtattcagg
    12781 gaaaggttat atcgccagag tggaaggttt actatctttg tcggaacttg attttggcta
    12841 agagaatatt cacggaagtg gaaattttta gtagttcatc aatccttttg cgcctgtgta
    12901 agtatatcac catattccct gtgcagcgcc ggaaatgggt atatctaaag tttttatgcc
    12961 gtgggattgt acatggtgta aaaggaatca gcggtaagtt tcactaagtg gacatagcaa
    13021 tgaaaaaact gtgttatttc ataaattcgg attggtattt tgatttgcat tggaccgatc
    13081 gtgcaattgc cgcccgagat gccggttatg agattcacat tattagccat tttgtcgatg
    13141 ataaaatagc ggaaaaattc aggacattag gttttgtttg ccataacatt ccacttgtcg
    13201 cccaatcatt caacgttttg attttctttc gggccttttc taaggctcgg aaaattattc
    13261 aaaacatcaa tccagatttg ttgcactgca ttaccatcaa gccctgtttg atcggcggtt
    13321 ttctggctaa aagtacccat cgtcctgtta ttctgagttt tgtcggtttg ggccgagtat
    13381 tttccgcaga gtctgcctgt cttaagctgc tgcgaagttt tactgttatg gcatataagt
    13441 atatcgccag taacaaatgc agtttgttta tgttcgaaca tgataaagac agagctaaac
    13501 ttgccgatct ggttggtatc gattacaaac agactattgt tattgatggc gcgggtatta
    13561 atccagagat ttacaaatac tctctggagc agcagcgtga tgttccggtc gtcctttttg
    13621 ccagccgtat gctgtggagt aaaggactgg gtgacctgat tgaagccaaa aaaatactga
    13681 gtaataaaaa tattcacttt acgctgaatg ttgccggtat tttagttgag aatgataaag
    13741 acgcaattcc gctggcgacg atacagaagt ggcaaagcga aggcgtgatt aactggctcg
    13801 gtcattgctc taatgtattt gatttaattg aagaatcaaa tatcgttgct ttgccgtcgg
    13861 tctacgccga aggcgtaccg cgtatcttgc tggaagcttc ctctgtcggg cgcgcttgta
    13921 tcgcttatga tgttggtggc tgtgatagct taattatcaa taactataat gggttgattg
    13981 taaaaagtaa atctgtcgag gaattagcgg agaaactcgg tttcctgttg gataacccag
    14041 aaacgcgcgt cgcaatgggt atcaatggca gaaaacgcat tcaagataaa ttctcgagtg
    14101 tgatgatcat taataaaaca ttaaaaacat atcgtgatgt tgttgaagag taatttttga
    14161 tgaagctcag tcgctttcat cttacccatc cttaataaac tggcaaccta caaattaaac
    14221 gctacccata aataaatata attacgttta taagctaagc gtcaaggtga ttgcatgtct
    14281 gaaaaagaga gtatagctgt ttccgttatt atccctgtgc acaatgcggc gggatatatt
    14341 tccgacacat tgagcactgt tttgtcgcaa acattaaatg atattgaaat cattattgtc
    14401 aacgattgtt ctgacgataa taccttagag attgtctcgg cgctggctga aactgattca
    14461 cgaattagag tcattaacaa cacgacaaat cttggtgggg gaggctcaag aaacatcggg
    14521 ttggatgctg ctaccggtga gtacgttatt tttcttgatg acgacgatta tgtcgataac
    14581 atgatgttgg agcgcatgta cgcacgtgct agcgatatac aagcggatgt cgttatctgt
    14641 cgcagccagt cttttgatcc cgccttgcag gtttatgctc cgatgccgtg gtcagtgcgc
    14701 caggagctgt tacctgatct caagtttttt tcatcccagg atattccctc tgactttttc
    14761 cgcacctttg tctggtggcc atgggacaag cttctcaggc gtcaatttat tactagtcat
    14821 cagcttcgct ttcaggaaat caggacgacc aacgatcttt tcttcgtctg cgcatttatg
    14881 ctcatggcga acagaatttc ggtcttaaat gaaacgttaa tttctcatac catcaaccgt
    14941 agtgaatccc tatccgccac gcgagcagag tcgcaccgct gtgctgttga agcattggtg
    15001 gcgcttaaag cgtttatctg tcagcagggg atgatggaac accgtctcag agattataaa
    15061 aactatgttg tcgtgttcct tgagtggcat ttaaacacga tttctggtcc ggcatttcac
    15121 ccgttttatc agcaagtgaa agagtttgtt gttgccctgg atgcaaagag tgatgatttt
    15181 tatgatgaat ttatcgccgc cgcccatcat aggatcacca cgctgtcagc tgaagagtac
    15241 ctattctctc ttaaagatcg ggttctgaag gaacttgagt ttttccaggc caggagctcc
    15301 gcattacagc aagaagttga gacgcttacc cactcattag ccgggcaaaa ggatgaaaat
    15361 gcgatattgc ataatcaatt gcatgagatt gaagagcggg ta
//
LOCUS       DAMZII010000106        15333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZII010000106 DAMZII010000000
VERSION     DAMZII010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.106"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(73..879)
                     /gene="trpA"
                     /locus_tag="POA77_003447"
     CDS             complement(73..879)
                     /gene="trpA"
                     /locus_tag="POA77_003447"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6366819.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(879..2072)
                     /gene="trpB"
                     /locus_tag="POA77_003448"
     CDS             complement(879..2072)
                     /gene="trpB"
                     /locus_tag="POA77_003448"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDI6366820.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2084..3445)
                     /gene="trpCF"
                     /locus_tag="POA77_003449"
     CDS             complement(2084..3445)
                     /gene="trpCF"
                     /locus_tag="POA77_003449"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDI6366821.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3446..5041)
                     /gene="trpD"
                     /locus_tag="POA77_003450"
     CDS             complement(3446..5041)
                     /gene="trpD"
                     /locus_tag="POA77_003450"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDI6366822.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5041..6603)
                     /gene="trpE"
                     /locus_tag="POA77_003451"
     CDS             complement(5041..6603)
                     /gene="trpE"
                     /locus_tag="POA77_003451"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI6366823.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6625..6718)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6695..6739)
                     /gene="trpL"
                     /locus_tag="POA77_003452"
     CDS             complement(6695..6739)
                     /gene="trpL"
                     /locus_tag="POA77_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDI6366824.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6877..7758
                     /gene="yciV"
                     /locus_tag="POA77_003453"
     CDS             6877..7758
                     /gene="yciV"
                     /locus_tag="POA77_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDI6366825.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKVQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7755..8375
                     /locus_tag="POA77_003454"
     CDS             7755..8375
                     /locus_tag="POA77_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI6366826.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8476..9348
                     /gene="rluB"
                     /locus_tag="POA77_003455"
     CDS             8476..9348
                     /gene="rluB"
                     /locus_tag="POA77_003455"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDI6366827.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9388..9978)
                     /gene="cobO"
                     /locus_tag="POA77_003456"
     CDS             complement(9388..9978)
                     /gene="cobO"
                     /locus_tag="POA77_003456"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDI6366828.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(9975..10733)
                     /locus_tag="POA77_003457"
     CDS             complement(9975..10733)
                     /locus_tag="POA77_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDI6366829.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10953..12002
                     /gene="sohB"
                     /locus_tag="POA77_003458"
     CDS             10953..12002
                     /gene="sohB"
                     /locus_tag="POA77_003458"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDI6366830.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12038..12289)
                     /locus_tag="POA77_003459"
     CDS             complement(12038..12289)
                     /locus_tag="POA77_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDI6366831.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12669..15266
                     /gene="topA"
                     /locus_tag="POA77_003460"
     CDS             12669..15266
                     /gene="topA"
                     /locus_tag="POA77_003460"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI6366832.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3713 a         3915 c         4114 g         3591 t
ORIGIN      
        1 aatgaattta ggatttttcc ggcttcatta aagaaagtta aaatgccgcc agcggaactg
       61 gcggctgtgg gattaactgc gcgtcgccgc tttcatcggt tgtacaaaaa ctttcagtgc
      121 cgccagcatt ttctctggct cattaatatg ttgctcgatg attttaacaa tggccgaacc
      181 agaaatcgcg cccgcagctc ctgcatcaat cgctgctttt acctgatccg gggcggaaat
      241 accaaacccc tgcaatggag gtgcagcgtt gtactctttc agcttcgcaa ccagatgatt
      301 gaggggtaac gcggcgcggt tttctgcgcc ggtcacgccc gctcgtgaca gcaaataggt
      361 gtaaccacga ccgtaagagg ctatctggcg cagcaggtcg tcatcggcat ttggcgggca
      421 gatgaagata ggtgcgacat tatggcgcaa cgcggcctgg cggaagggcg cggactcttc
      481 aattggcaca tcggcaacca gcaccgaatc gacgccgact ttctcgcact gggcataaaa
      541 ctcatcaatg cctttgttaa acaccagatt ggcatacatc aacaggccaa tgggaatggt
      601 cgggtgtttc tggcgaatca gtgccagcat ttcaaaacat tgtgccggag tcacacctgc
      661 cgcaaaggcg cgcagagtgg cgttttgaat ggtcgggcca tccgccagtg ggtcggagaa
      721 ggggatacct aactccagcg cgtcagcacc ggcttcaatt agcgtatcga taattttcaa
      781 tgactgctca atgcccggat caccgagcgt gacgaaagga acgaatgcgc cttctttgcg
      841 ctccttcaac tgggcaaaca gagattcgta gcgttccatc agatttcccc tcgtgctttc
      901 aaaatatcgt gaacggtgaa gatgtcttta tcgccgcgac cggaaaggtt aaccaccagt
      961 agctgctctt tatccgggtt ttcgcgcatc attttcaacg catgggccag ggcgtgggag
     1021 gattccagcg ccgggatgat cccttcgtgc aggcacagcg ttttgaaggc ttcaagggct
     1081 tcatcatcgg taatagacac gtaatcagcg cgtccagtgc tgttaagata cgcgtgttgt
     1141 gggccgacag acgggaaatc cagtccggcg gagatggagt aagattcttc aatctgcccg
     1201 tcttcggttt gcatcatcgg cgctttcata ccgaaataga tacccacgcg accatgtttt
     1261 agcggtgcgc cgtgctcgcc agtttcgata ccgtgaccac ctggctccac accaatcagg
     1321 ccgacgttgg tttcattgat gaaatcagca aacatgccga tggcattcga accgccgcca
     1381 acacaggcga taacggcatc cggcaggcga ccttctcttt ccagaatctg cgctttggtt
     1441 tcttcgccaa tcatccgctg aaactcacgc acaatggtcg gataaggatg cgggccagct
     1501 gcggtgccca gcatatagtg cgcggtttcg taactaccgg accagtcgcg cagcgcctcg
     1561 ttacaggcat ctttcagcgt cgcggaaccg ctatgcaccg ggatcacttc cgcacccatt
     1621 aagcgcatac gaaaaacgtt aggcgactgg cgttcaacgt ctttggcacc catataaata
     1681 cggcatttca ggccgagcag ggcgctggca agggccgacg ccacgccatg ctgaccggca
     1741 ccggtttcgg cgatgatttc ggttttaccc atccgcttcg ccagcaacgc ctgccccagc
     1801 acctggttag ttttatgcgc gccgccgtgc agcaaatctt cacgcttgag atacagcgtg
     1861 gtgttcgtcc cggctgtaat gttctggcat ttggtcagcg cggttggacg cccggcatag
     1921 tttttcagca ggtcgttgaa ctgagcctga aattcaggat ctttttgcgc actgacaaaa
     1981 gcttcttcca gctggcgcag agcaggcatc aggatttgtg gcacgtacat gccgccaaac
     2041 tcaccaaaat aggggttaag taatgttgtc attgttcctt tccttaatat gcgcgcagcg
     2101 tctggaaaac cgaggccaaa agacgtgcgt ctttgatgcc cggttgcgac tctacagcag
     2161 aattaaaatc aagtccggcg cagccggttt gtgccgcttc cacgcagtta tctgcgccta
     2221 agccccccgc cagcagaacg ttgccaagcg attgaccatt taatagtgac cagtcaaaac
     2281 gttgcccgct tccaccctgg ccgttgtcta aaacatattt atcaacgtgc tgaaactcgc
     2341 gggcgggcag ggtttcaccg acgcttaatg ctttccagat ggcaacatgt gctggcagag
     2401 cttcacgcag cgtatcgata tacagctgtt cttcattacc atgcagttgc actgccgcca
     2461 gcgataacac cttagctttg tccaccacat cggcaatatc gtgattgcgg aacacgccaa
     2521 catactgcaa cggtgccgca gccatcactt cctgcgcctg ttcaacgttg acgcaacgcg
     2581 gtgatgtcgc aacaaaaatc aacccaccgt aaatcgcgcc cgcgtcataa gctgctttag
     2641 catcttgccc acgcgtcagg ccacatactt tattctcacc cagcaacacc cggcgcacgg
     2701 cggcgtgcaa atcgtcatgg gccatcaacg ccgaaccaat cagaaaaccg ttagcgaagt
     2761 ggcttaactc gcgcacctga gcgtaagtat tgatgccgga ttcgctgatt accgtcacgt
     2821 tgtgccccag tttcggcgca agctcgcggg tacggttgag atcaatcgac aaatcacgca
     2881 gatcgcggtt gttgatgcca acgacctttg cccccaatgc aatggcacgc tccagttcct
     2941 cttcattact gacttcggtc agcacaccca tctccagact gtgggcgacg gcggcaagct
     3001 ggcgatattg ttcgtcatcc agtactgaaa gcattaataa gcaggcatca gcctggtaat
     3061 agcgcgccag atagatctgg taaggatcga taatgaagtc tttacataaa atcggctgcg
     3121 gggcgatttg gctgacgatg gggaggaaat caaagctccc ctgaaaatat ttctcatcag
     3181 tcagcaccga aattgccgaa gcgtaatgtt tataaatggc ggcaatgcgt gctggatcga
     3241 aatcatcacg gatcacgcct tttgacggcg acgctttctt gcactccaga ataaacgccg
     3301 tgcgcgcacc ctgtagcgca tcataaaaat gtcgcgtgct cggctgaacc tcattctgaa
     3361 aactggccag cggttgctgc tgtttgcggg tttctaccca aatcgccttg tctgcgacga
     3421 ttttcgctaa aacggtttgc atcatttacc ctcgtgccgc cagtgcggta actctgtcgt
     3481 aagcggaacc actgcgcagt acctcaagaa cggtttgcgc attggcttgc agatcttcat
     3541 ggccatgcag gcgcattaac atggcgacgt tcgcagcgac ggctgcttca tgggctgcgt
     3601 cgcctttacc ttgtaacaag cgtgttaaaa tgtcacggtt ttcttccggt gttccgcctg
     3661 ccagttgctc ctggtggtag ggagtcaggc caaaatcttc agcggtcaat tgatagctct
     3721 taatttcgcc gtcatgcagc tcggcaacga ttgtcggcgc gtgtaatgaa acttcatcca
     3781 tcccgccgct gtgcaccacc gccgcgcgtt gataccccag cacgcgcaag gtttcggcaa
     3841 tcggcagcac cagttccgga ctataaacac caattaacgc cagcggcgga tgcgccgggt
     3901 taatcaatgg ccccagcaca ttgaacaggg tgcgggtttt cagttgctgg cgaaccggca
     3961 tcgcgtggcg gaatccggtg tgatacttcg gcgcaaagag gaaacataca cctaactcat
     4021 ccagcgcctg gcgcgattta tcggcgttca tatcaagatt aataccgaac gccgccagca
     4081 gatcggacga accagattta ctggagacgc tacggttgcc gtgtttcgcc actttcagcc
     4141 cacaggccgc ggcgacaaac gcactggcgg tagaaatatt gatactgttg ctgccgtcac
     4201 cgccagtacc gacgatatca gcaaacagat aatccgggcg cgggaacggc gcggcgtttt
     4261 ccagtagcgc ggttgctgct ccggcgatct cgttcgggtg ctcaccgcga attttcatgc
     4321 tcaccagcgc cgccgccagt tgttccggct tcagctcgcc acgcaccacc gctgaaaaca
     4381 gctggtggct ttcttgttgg ctaagcgtct gcgcctgata cagtttttcc agaatcggtt
     4441 gcagcgtgtt ggctggctct agtttctgct gcgcccaggc cagcgtttgt tccagcaggc
     4501 gagcgccctg ggtggtgaga atggattccg gatggaactg gaatccacaa acgcgatccg
     4561 catcgtgacg tactgccatc accatgccat taaaatgggc gttgatggtt aaaccggccg
     4621 gaatgttact gccaaccagc gagtgataac gcgccaccgg cagcgggttt gttaatccgg
     4681 caaacatcgc ctgaccgtca tgttcaatgc tcgacgcttt accgtgaaga atttcgcccg
     4741 cctgaccgac atagcccccg taagcttcga caattgcctg atgtccgagg caaatgccaa
     4801 taatgggcag cttgccacgc aagcgggtga ggagttccgg catacaaccg gcttcgctcg
     4861 gcacaccggg gccaggagaa agcatcagta ccggattgct catggtcgcc aggcgttcaa
     4921 ttaaggtctg cgccggaata tggttgcggt aaatcaccac gttatgccca ttgctgcgca
     4981 actgatctgc caggttgtac gtaaaagagt cgatattatc gagcagcagt atgtcagcca
     5041 tcagaaagtc tcctgtgcat gatgcgcggt ggcaatagcg cgcagtacag cgcgggcttt
     5101 attacgggtt tcgtcggctt ccgactgcgg aatagaatca aggactacgc cagcaccggc
     5161 ttgcacggtg gcgataccgt tttccaccag cgccgagcgg atcacaatgc aggtgtcgag
     5221 atcgccgtgc gcggtgaaat aacctaccgc gccgccgtag ctgccgcggc gacgaccttc
     5281 cgcctcggca attaactgca tggcgcgcac tttcggcgca ccgcttaacg tccccatatt
     5341 catacaggcg cggtaagcgt gtagggcgtc gagatcgtgg cgcagttcac cgaccacgcg
     5401 ggagaccagg tgcatcacgt aagagtaacg gtcaactttg gtgaggtcgg cgacgtagcg
     5461 gctgccgggg gtgcaaatgc gtgccagatc attacgggca agatccacca gcatcagatg
     5521 ttcagaaagc tctttatgat cggtacgcat ttccagttcg atgcggctgt cgaggtctct
     5581 gtccagcgag ccatcggcac gacgaccgcg tggacgtgtt ccggcaatcg ggtagatctc
     5641 aatctggcgg ctggtggcgt catacttgag cgaactttcc ggcgacgcgc caaacagggt
     5701 gaaatcatta tcctgcataa aaaacatgta cgggctggga ttactctttt tcagcacgta
     5761 ataggccgcc agcggtgacg ggcagggcag agagaaacgg cgagatggca ccacctggaa
     5821 aatttctcca gcgcgaatcg ctttttgcaa caaacgcact acgccaccga actcttcatc
     5881 gctctgatta cattcacaac gcatatgcgg cacggaaacc accggcagcg gcggcgcggc
     5941 ttcggtcagt tgctgacgta gttcgttcag gcgagcagtg agacgttgtt tttcttcttc
     6001 attcggagca aacaggctgg cctgaatacg ggtgcttttt ttctgatggt caatcaccat
     6061 cagcgtttca gcgagataaa aacagaaatc agggcagtta ttttccgctg acagttgcgg
     6121 taaatcttca aatcccgcca caaggtcata agagaacagg ccgccgaaga acattgcttc
     6181 tcgttcttcc ttcggtacat tcaacagatt ctgcaataaa cggaaagcgt caaaaaccga
     6241 aagggagcat aagcgagcgt cttcatccag cagtggactg acagggggga agcgcagcac
     6301 acggcagttt ggtaattgtt cattttccac acccgcaggc agggcgttat ccagtagtgt
     6361 cagcagggct tcgccgttgc cggaaagtgc ctggattgtg acagtgtcac ctaaagctgt
     6421 aatgcgcagc gcactgtcta ccagcagcag gctttttaaa tcatctttgc tgtcgatatc
     6481 tgcggattcc agcagcagcg ttgccggacg atccccacac aactggtgaa aaagcgcggt
     6541 gggattgtcg cgataagcgc cttcgcaggt tagcagttcg agagtcggtt tttgtgtttg
     6601 cattgttatt ctctaatttt gttcaaaaaa aagcccgctc attaggcggg ctgggtatct
     6661 gattgcttta cgcatggtga atacactgcc cgtttcagga agtgcgccac caacctttca
     6721 gtacgaaaat tgctttcatt gtcgataccc tttttacgtg aacttgcgta ctagttaact
     6781 agttcgatga ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt
     6841 cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg
     6901 atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg
     6961 gtgcaccgtg cagtcgagat gcgcgtcggc accctggcga tcaccgacca tgacaccaca
     7021 gctgcaatcg cgccagcaag agaggaaatt tcacgttcgg gactggcgtt gaatcttatt
     7081 cccggcgtgg aaatttccac ggtctgggaa aatcatgaaa ttcatattgt cgggctgaat
     7141 attgatatta ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga acggcgcaat
     7201 cagcgggcgc agctgattgc cgaaagactt gaaaaagtgc aaatacctgg ggcgctggaa
     7261 ggcgcacaac gactggcgca gggcggcgca gtgacgcgcg gtcattttgc gcgtttcctt
     7321 gttgagtgcg gcaaagccag ttcaatggcg gatgtcttta aaaagtatct ggcgcgcggg
     7381 aaaaccggat acgttccgcc acagtggtgt acaatagaac aagctattga tgtcattcat
     7441 cattctggcg gtaaggcggt gctagctcat cctgggcggt acaatcttag tgctaaatgg
     7501 ctgaaaagat tggttgcgca ttttgccgaa caccacggtg acgcgatgga agtcgcgcag
     7561 tgccagcaat cgcccaatga acgtacccag ctggcggccc ttgcgcgtca gcatcattta
     7621 tgggcatcac aaggatctga ttttcatcag ccatgcccgt ggatcgaact gggtcgtaaa
     7681 ctctggctgc ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc gcagaacacc
     7741 acagagaggg aattatgagc cagttttttt atattcatcc tgataaccca cagcaacgtc
     7801 tgatcaacca ggcggtggag atcgtgcgta aaggcggggt gattgtttat ccaactgatt
     7861 ccggctatgc gctcggctgt aaaattgaag ataaaaacgc gatggagcgt atttgtcgta
     7921 ttcgccagct gccggacggt cacaacttta ccctgatgtg tcgcgatctt tctgaactct
     7981 cgacctattc atttgttgat aacgttgcgt ttcgtttaat gaaaaacaac acgccgggca
     8041 actatacctt catcttgaag gggacgaagg aagtgccacg ccgtctgttg caggaaaaac
     8101 gcaaaaccat cggtatgcgt gtgccttcta acccgattgc gcaggcgttg cttgaagcac
     8161 tgggcgaacc gatgctttcc acttcgctaa tgctgccagg cagcgaattt accgaatccg
     8221 atcccgaaga aattaaagac cgtctggaaa aacaggtaga tttgattatt cacggcggtt
     8281 atctcgggca gaaaccaaca acggttattg atcttaccga tgatacgccg gtcgtggtgc
     8341 gtgaaggcgt aggtgatgtg aagcctttct tataagtgcg taactctgta tactacgcgg
     8401 ccttaaaatg gcgctatacg gcgccattga ttctcctgtt cgacgcctgt gaaggcgaca
     8461 ctaaaaggaa gctctatgag cgaaaaatta cagaaagtgc tggcccgcgc tggccacggc
     8521 tcccgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga tggcaaaatt
     8581 gctaaactcg gcgatcgcgt tgaagttacc cctgggctga aaattcgtat cgatggtcat
     8641 ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta ttacaaaccg
     8701 gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc cgacggtgtt tgaccgtctg
     8761 ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa cacctgtggt
     8821 ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcatcc aagccgtgaa
     8881 gttgaacgtg aatacgccgt gcgtgtattt ggtcaggttg acgacgcgaa actgcgtgat
     8941 ttgagtcgtg gcgtgcaact ggaagatggt ccggcagctt ttaaaaccat caagttcagc
     9001 ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg taaccgtgaa
     9061 gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg tgttcgttac
     9121 ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct ggacctcgcc
     9181 cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc taaagtcgct
     9241 gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt gaaacgtcac
     9301 agtcaggtga gcggcggtcg tcgttctggc ggacgtaata acggttaatc aaagtatgcc
     9361 ggacgtcata tccggcattt ttacagatta gtaatcaatc cctatctgcg cttttacacc
     9421 ggcatcaaac gcatgtttga cggggcgtaa ttcacttacc gtatctgcca gttcaagaat
     9481 atcccgatga caaccacgac ccgtgataat caccgtctgt tgatgtggac gttcatttaa
     9541 cgcctgcacc acttcttcca gtggcaaata gtcatacgcc accatatacg tcagttcatc
     9601 aagcaaaacc atatccagtg aggaatcagc aagcatccgc tttgcatgtt gccagacttc
     9661 gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc ccgttgccat
     9721 cacctgaaac tcaacgccat gtggctccag cagattgcgt tcgccattag gccaggtgcc
     9781 tttaataaac tgcacgacgc ctactttttt tccgtgacca actgcgcgtg ttgccgtacc
     9841 aaatgccgcg gtggttttgc cttttccatt gccggtaaag acgatgataa taccgcgttc
     9901 atcctgggcc tgggccacac gagcatctac tttttctttc actcgctgct gacgctgttg
     9961 gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc gtcaaaggtc
    10021 atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg catgatatca
    10081 gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgctggcacg cattgcggtg
    10141 cgcgtaccgc ctgggttaat gcagttgaca cgcaggcgct gctgatattc atcggccagt
    10201 acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcacccca gttagctcgt
    10261 ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga tttgagtaat
    10321 aaaggaagca gcgcctgggt gagcaaaaag gtggcattaa cgttgacctg catgacgtcc
    10381 tgccagacct gcggattttg ttcgctcatt gggcaaacat cgccgagcaa tccggcatta
    10441 tgcaaaacac catccagacg cggataatta acggtaatgc gctgtgccag ttgttggcaa
    10501 tcttcggacg tgcaggtcag caaatcgaga ataaaccact gtggctgacg tccagtttct
    10561 tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa cagaatcact
    10621 gtcgcaccat agcgtgcata cgtcatcgcg gcttcacggc caatcccatc gctggctccc
    10681 gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg catggctact
    10741 cctcaacgac gttgtctgtc gcgctgccag tgtactttat gactttgggg ctttatgcct
    10801 gaaacagaag ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg atactttgtc
    10861 atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg taaactgtga
    10921 gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa tatggtttgt
    10981 ttttggcgaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc attattgtca
    11041 atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat ctcagcgaac
    11101 agtataagga gatgaaagaa gaactggccg cggcgctgat ggactcacat cagcaaaaac
    11161 agtggcacaa agcgcagaag aaaaagcaca agcaagaagc gaaagcagca aaagcgaaag
    11221 ccaaactggg cgaggtggca actgacagta aacctcgcgt ctgggtgctg gattttaaag
    11281 gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg gtactcgcag
    11341 cattcaaacc gcaggatcag gttgtgctcc gtctggaaag ccctggtggc atggtgcatg
    11401 gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt cctttaactg
    11461 ttacggtaga caaagtcgct gccagcggcg gttacatgat ggcctgtgtg gcggacaaaa
    11521 ttgtttccgc accgtttgct attgtgggtt ccattggggt ggtggcgcaa atgcccaact
    11581 ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacactgcc gggcagtata
    11641 agcgtactct gaccttgctg ggtgaaaata ccgaagaagg gcgggagaaa ttccgcgaag
    11701 agttgaacga aacgcatcag ttgtttaaag attttgtgaa gcgtatgcgt ccgtctctgg
    11761 atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta gagaaaggcc
    11821 tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa ggccgtgaag
    11881 tggttaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc ggcagcgcgg
    11941 cagagagcgc cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag ccattgatgt
    12001 aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta atcaaccaga tgatattttt
    12061 ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt ttggctgttg
    12121 gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca ttacgctgcg
    12181 tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg attttgttgg
    12241 cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt atttactcct
    12301 taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc acgctggtgg
    12361 caagagcgcc ttactggtaa ctttggattt tgcatgctaa taaagttgcg tatcggattt
    12421 tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga cattcgacca
    12481 aaattccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc acctgaattt
    12541 tcgtgaacag agtcacgaca aggggttgat atccgcagag aacgagtcca tatcggtaac
    12601 tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc aaattaggta
    12661 aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc aaaacgatca
    12721 acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc cgcgatttgc
    12781 cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag acggctaaaa
    12841 agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac ccgtggcaca
    12901 attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct gaactgaaac
    12961 aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc gaaggggaag
    13021 ccattgcatg gcacctgcgg gaagtgattg ggggtgatga tgcgcgctat agccgagtgg
    13081 tgtttaacga aattactaaa aacgcgatcc gccaggcatt taataaaccg ggtgagctga
    13141 atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg gtggggtata
    13201 tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct gtctgccggt cgtgtgcagt
    13261 cggtggcggt tcgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc gtgccggaag
    13321 agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg gcgttacagg
    13381 tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact caggctgcgg
    13441 taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa ccgacaacca
    13501 gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc acccgtcttg
    13561 gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca ggctatatca
    13621 cttacatgcg taccgactcc actaacctga gtcaggacgc ggtaaatatg gttcgcggtt
    13681 atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag tacgccagca
    13741 aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat gtgatggcgg
    13801 aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc tggcgtcagt
    13861 tcgtagcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc gttggtgcgg
    13921 gcgatttccg cctgaaagca cgcggtcgta ttttgcgttt tgatggctgg acaaaagtga
    13981 tgcctgcgtt gcgtaaaggc gatgaagatc gcatcttacc agcagttaat aaaggcgatg
    14041 ctctgacgct cgttgaactt acaccagccc agcactttac caagccgcca gcccgtttca
    14101 gtgaagcatc gctggttaaa gagctggaaa aacgtggtat cggtcgtccg tctacctatg
    14161 cgtcgatcat ttcgaccatt caggatcgtg gctacgtgcg agtagaaaat cgtcgtttct
    14221 atgcggaaaa aatgggcgaa atcgtcaccg atcgccttga agaaaatttc cgcgagttaa
    14281 tgaactacga ctttaccgcg cagatggaaa acagcctcga ccaggtggca aatcacgaag
    14341 cagagtggaa agctgtactg gatcacttct tctcggattt cacccagcag ttagataaag
    14401 ctgaaaaaga tccggaagag ggtggtatgc gcccgaacca gatggttctg accagcattg
    14461 actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc gagcaccggg gtattccttg
    14521 gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt aacctggtgc
    14581 cggaaaacga agtgctgaac gtgctggaag gcgaagatgc tgaaaccaac gcgctgcgcg
    14641 caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc gatccgaaac
    14701 gtaagttgca tgtctgtggt aataacccaa cctgcgacgg ttacgagatc gaagagggcg
    14761 aattccgcat taaaggttat gacggcccga tcgttgagtg tgaaaaatgt ggctctgaaa
    14821 tgcacctgaa aatggggcgt ttcggtaaat acatggcctg caccaacgaa gagtgtaaaa
    14881 acacacgtaa gattttacgt aacggcgaag tggcaccacc gaaagaagat ccggtgccat
    14941 tacctgagct gccgtgcgaa aaatcagatg cttatttcgt gctgcgtgac ggtgctgccg
    15001 gtgtgttcct ggctgccaac actttcccga aatcgcgtga aacgcgtgcg ccactggtgg
    15061 aagagcttta tcgcttccgc gaccgtctgc cggaaaaact gcgttatctg gccgatgcgc
    15121 cacagcagga tccggaaggt aataagacca tggttcgctt tagccgtaaa accaaacagc
    15181 aatatgtctc ttcggaaaaa gacggaaagg cgactggctg gtcagcattt tatgttgatg
    15241 gcaaatgggt tgaaggaaaa aaataacctt taattctgtc aggtttttat aaacaaaggg
    15301 tcgcgaaagc ggcccttttt tattgcatat tat
//
LOCUS       DAMZII010000107        15238 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZII010000107 DAMZII010000000
VERSION     DAMZII010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.107"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..1782)
                     /locus_tag="POA77_003461"
                     /pseudo
     CDS             complement(1..1782)
                     /locus_tag="POA77_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2306..4234
                     /gene="thrS"
                     /locus_tag="POA77_003462"
     CDS             2306..4234
                     /gene="thrS"
                     /locus_tag="POA77_003462"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI6366833.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4238..4780
                     /gene="infC"
                     /locus_tag="POA77_003463"
     CDS             4238..4780
                     /gene="infC"
                     /locus_tag="POA77_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI6366834.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4877..5074
                     /gene="rpmI"
                     /locus_tag="POA77_003464"
     CDS             4877..5074
                     /gene="rpmI"
                     /locus_tag="POA77_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI6366835.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5127..5483
                     /gene="rplT"
                     /locus_tag="POA77_003465"
     CDS             5127..5483
                     /gene="rplT"
                     /locus_tag="POA77_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI6366836.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5600..5724
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5606..5650
                     /gene="pheM"
                     /locus_tag="POA77_003466"
     CDS             5606..5650
                     /gene="pheM"
                     /locus_tag="POA77_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDI6366837.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5934..6917
                     /gene="pheS"
                     /locus_tag="POA77_003467"
     CDS             5934..6917
                     /gene="pheS"
                     /locus_tag="POA77_003467"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI6366838.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6932..9319
                     /gene="pheT"
                     /locus_tag="POA77_003468"
     CDS             6932..9319
                     /gene="pheT"
                     /locus_tag="POA77_003468"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI6366839.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLAGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9324..9623
                     /gene="ihfA"
                     /locus_tag="POA77_003469"
     CDS             9324..9623
                     /gene="ihfA"
                     /locus_tag="POA77_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDI6366840.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9724..10704
                     /gene="btuC"
                     /locus_tag="POA77_003470"
     CDS             9724..10704
                     /gene="btuC"
                     /locus_tag="POA77_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDI6366841.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10767..11318
                     /gene="btuE"
                     /locus_tag="POA77_003471"
     CDS             10767..11318
                     /gene="btuE"
                     /locus_tag="POA77_003471"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDI6366842.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11318..12067
                     /gene="btuD"
                     /locus_tag="POA77_003472"
     CDS             11318..12067
                     /gene="btuD"
                     /locus_tag="POA77_003472"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDI6366843.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12145..12609
                     /locus_tag="POA77_003473"
     CDS             12145..12609
                     /locus_tag="POA77_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6366844.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12856..13569
                     /gene="ydiV"
                     /locus_tag="POA77_003474"
     CDS             12856..13569
                     /gene="ydiV"
                     /locus_tag="POA77_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDI6366845.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13632..15068
                     /locus_tag="POA77_003475"
     CDS             13632..15068
                     /locus_tag="POA77_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDI6366846.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLHRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15072..15191)
                     /gene="hemP"
                     /locus_tag="POA77_003476"
     CDS             complement(15072..15191)
                     /gene="hemP"
                     /locus_tag="POA77_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDI6366847.1"
                     /translation="MRRISSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
BASE COUNT         3882 a         3451 c         3997 g         3908 t
ORIGIN      
        1 ataatgatta tgatgaatgg ctatatgaac agctgacatg ttttcatgat ttttagcggc
       61 caacagtgtg aataattgac gttgactaaa agaatatttt tttgcaaaag tactcagtgt
      121 tgatatataa gataacacga cgtctttatt acccttgctc atggcgatgt ataaagcagg
      181 ggttccatgt gctagctttg ctcaacttgt atttaaaggc gatgccatta attttagaga
      241 ggaaccgtgt gacacacaaa gggtgattat tttccattgc ggcgtataat cctggctcac
      301 catcatgtat tttcatggct aaaatactca tcgccagttt tttgtttaaa agctcttgtg
      361 ctattggagt ggttaatgca ttcagaaaga tatcgattat ttcatcgaat ccatacaaca
      421 tggagatata aagtccaggt gtttcgttag cacttttagt ttgcagtaat ttaaccagat
      481 tatcttcatg aatgatgtga ttatctacca gtgactgaat ttcttgcata aatattttca
      541 cgttgtcagt atgtctgttc atcatagcca aaaataaagc aggtgtacca acgctattct
      601 gcgcagttat tatttcaatg atttcttttt cagggagtat gggcaactgt ttgaataatg
      661 taataagctg ttgagtctgg cctgtagcca ttattaacaa cattgccgat ggatgtatag
      721 gggcaaatgg cattcgtgat gcaatacccg cgaaaatagc ccctggaact cccacaggta
      781 ataggggcag aatagcaatt gcacagttgc gaacatacct atcagcccag gtctgagcat
      841 cgggacctga actgatgaac ttatcaagcg aaagaaagcc ccgtgctgtc ctgtgggtgt
      901 ctctgacggc aacgttggta tcgttcgggt cgtaaacgga cacaacatag tttaattgtc
      961 catacactga ctgctttatt cgtagcctta ctgacaaagt atgattgtct atatataaaa
     1021 tcgcggacat atgactgaca ttgtttgcca tcattcgaca aaagaaaata tctaaagcga
     1081 agccaaaatc cttactcgcg ataacgcgac tccctgcagg aggatggcga agtaatgctc
     1141 tgaactccgc gtcccggact aaggggacat gctgctgtat ggattcaacg gaggagtaat
     1201 gtttaatcag gttgactttg ccttttccat ttctcaatga atcaagggcg tattgcaatg
     1261 acagatggcg acaaaaaata gcgtcagaag gagattgcaa aaaacatgca ctaccattga
     1321 gatttactgt tgggtgtata gttttttctc tatggatttt tttagacagt ttcatacaat
     1381 ggttacgatc ttcagcagtt aactgttcct gttcaatata atccagtatg cttcgcaaat
     1441 ggttagcttt aataggcgtg gtttccagta ataaggaaat gaaatctttt tatagtgtaa
     1501 tgtaatgagt atattttctg catggatggg tgaagtggca aagttaatgg taaaaagggc
     1561 agtggaataa caattttgtg atatttttcg acagtttgcg gtaaattcct gaataacaca
     1621 gtatgacagc atcgaactta agtcagaggt atcaagtagg gtgatgagct ggcgcgcaaa
     1681 tttattaata ggctctgatg aaggtgttgg tatgtcgcta tcgatgtgca atctatgagg
     1741 agacttataa tgagttctga taatgatatc gttttgactc ataatctatt cgccttatat
     1801 atatctggag gttttgagga tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa
     1861 aaattgttat aagaattgtg tcacttttat cattgtgata tgaccacttc gtcattaatc
     1921 tcatgagcgt ttaaaatact accgccaaat cacgcaacga actttgctgg attcagttta
     1981 tgctgctgta aatccgctcg cagtaaacct ttcagacgca ccgtgatgtt atcagtggtt
     2041 ctttaagcgt tttgctggtg tactcactac aaacgaattg cgaatcgaat caatgtgaaa
     2101 cggaaaggta caatctccct ttttttagtg ttaacatcgc tcaaccgggt ggaaggtgag
     2161 aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga
     2221 tggtttcgca atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg
     2281 tcaccactgc aaataaggat ataaaatgcc tgttataact cttcctgatg gcagccaacg
     2341 ccattacgac cacgctgtaa gccccatgga tgttgcgctg gacattggtc caggtctggc
     2401 gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt gatgcttgcg atctgattga
     2461 aaacgacgca caactgtcca tcattaccgc caaagacgaa gacggtctgg agatcattcg
     2521 tcactcctgt gcgcacctgt tagggcacgc gattaaacaa ctttggccgc ataccaaaat
     2581 ggcaatcggc ccggttattg acaacggttt ttattacgac gttgatcttg accgcacgtt
     2641 aacccaggaa gatgtcgaag cactcgagaa gcggatgcat gagcttgctg agaaaaacta
     2701 cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt gaaactttcg ccaaccgtgg
     2761 ggagagctac aaagtctcca ttcttgacga aaacatcgcc catgatgaca agccaggtct
     2821 gtacttccat gaagaatatg tcgatatgtg ccgcggtccg cacgtaccga acatgcgttt
     2881 ctgccatcat ttcaaactaa tgaaaacggc aggggcttac tggcgtggcg acagcaacaa
     2941 caaaatgttg caacgtattt acggtacggc gtgggcagac aaaaaagcgc ttaacgctta
     3001 cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt aaaatcggta aacagctcga
     3061 cctgtaccat atgcaggaag aagcgccggg tatggtattc tggcacaacg acggctggac
     3121 catcttccgt gaactggaag tgtttgttcg ttctaaactg aaagagtacc agtatcagga
     3181 agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa aaaaccggtc actgggacaa
     3241 ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt gaatactgca ttaagccgat
     3301 gaactgcccg ggtcacgtac aaattttcaa ccaggggctg aagtcttatc gcgatctgcc
     3361 gctgcgtatg gccgagtttg gtagctgcca ccgtaatgag ccgtcaggtt cgctgcatgg
     3421 cctgatgcgc gtgcgtggat ttacccagga tgacgcgcat atcttctgta ctgaagaaca
     3481 aattcgcgat gaagttaacg gatgtatccg tttagtctat gatatgtaca gcacttttgg
     3541 cttcgagaag atcgtcgtca aactctccac tcgtcctgaa aaacgtattg gcagcgacga
     3601 aatgtgggat cgtgctgagg cggacctggc ggttgcgctg gaagaaaaca acatcccgtt
     3661 tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa attgaattta ccctgtatga
     3721 ctgcctcgat cgtgcatggc agtgcggtac agtacagctg gacttctctt tgccttctcg
     3781 tctgagcgct tcttatgtag gcgaagacaa tgaacgtaaa gtaccggtaa tgattcaccg
     3841 cgcaattttg gggtcgatgg aacgtttcat cggtatcctg accgaagaat tcgctggttt
     3901 cttcccgacc tggcttgcgc cagttcaggt tgttatcatg aatattaccg attcacagtc
     3961 tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg ggcattcgtg ttaaagcaga
     4021 cttgagaaat gagaagattg gctttaaaat ccgcgagcac actttgcgtc gcgtcccata
     4081 tatgctggtc tgtggtgata aagaggtgga atcaggcaaa gttgccgttc gcacccgccg
     4141 tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc gagaagctgc aacaagagat
     4201 tcgcagccgc agtcttaaac aattggagga ataaggtatt aaaggcggaa aacgagttca
     4261 aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc caggaagttc gcttaacagg
     4321 tctggaaggc gagcagcttg gtattgtgag tctgagagaa gctctggaga aagcagaaga
     4381 agccggagta gacttagtcg agatcagccc taacgccgag ccgccggttt gtcgtataat
     4441 ggattacggc aaattcctct atgaaaagag caagtcttct aaggaacaga agaaaaagca
     4501 aaaagttatc caggttaagg aaattaaatt ccgtcctggt acagatgaag gcgactatca
     4561 ggtaaaactc cgcagcctga ttcgctttct cgaagagggt gataaagcca aaatcacgct
     4621 gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt atggaagtgc ttaatcgcgt
     4681 gaaagacgat ttgcaagaac tggcagtggt cgaatccttc ccaacgaaga tcgaaggccg
     4741 ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa ggccttcaag tagcactgtc
     4801 tgtggagcct tcgggcttca caggctttgt tcgcctttgt ttcgtttatt aacaatgcga
     4861 agtggaagtt attaaaatgc caaaaattaa gaccgtacgc ggtgctgcta agcgcttcaa
     4921 aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac ctgcgtcaca ttctgaccaa
     4981 aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc atggtttcca aaggcgatct
     5041 gggcctggta atcgcgtgcc tgccgtacgc ataagccgtt aacgttttta actttttaat
     5101 tagaatatag atacaggaga gcacatatgg ctcgcgtaaa acgtggtgtt attgcacgcg
     5161 cacgtcacaa gaaaattttg aaacaagcta aaggctacta cggtgcgcgt tctcgcgtat
     5221 accgcgttgc cttccaggct gttatcaaag ctggtcagta tgcttaccgt gaccgtcgtc
     5281 aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat caacgcagca gcacgtcaga
     5341 acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa agcctctgtt gaaatcgacc
     5401 gtaagatcct ggctgatatc gcagtattcg acaaagtagc gttcaccgct ctggttgaaa
     5461 aagcgaaagc agctctggca taagccagtt gaaagaggga gctagtctcc ctcttttcgt
     5521 ttcaacgcca tcaaaacatt gacttttatc gccgtagcct tttcaataaa ggtcttttga
     5581 agagtaccaa aaggtaacgc aagcaatgaa tgctgctatt ttccgcttct ttttttactt
     5641 tagcacctga atccaggagg ctagcgcgtg agaagagaaa cggaaaacag cgcctgaaag
     5701 cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa ataaaaagcc tgtctcatca
     5761 ggtgtaattg tcgcagccag tctggacacg gccagcgcgg agaaaccgga gcgtatttaa
     5821 gtacgtgaga atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg
     5881 ataggctcta agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac
     5941 atctcgcaga actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg
     6001 cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga
     6061 cgaccctgcg tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag
     6121 cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgcagcgc
     6181 tgaatgcgcg tctggcggcg gaaacgattg atgtctccct gccaggtcgt cgcattgaaa
     6241 acggcggtct gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc
     6301 ttggctttac cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc
     6361 tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacacta
     6421 cccgcctgct gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc
     6481 caccgattcg tatcatcgcg cctggccgcg tttatcgtaa cgactacgac cagactcaca
     6541 cgccgatgtt ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc
     6601 tgaaaggcac tctgcacgat ttcctgcgta acttctttga ggaagatttg cagattcgct
     6661 tccgtccttc ctacttcccg ttcaccgagc cttctgcaga agttgacgtc atgggtaaaa
     6721 acggtaaatg gctggaagtg ctgggttgcg ggatggtgca tccgaacgtg ctgcgtaacg
     6781 ttggcatcga cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga
     6841 ctatgttgcg ttacggcgtc accgacctgc gttcattctt cgaaaatgat ctgcgtttcc
     6901 tcaaacagtt taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga
     6961 atgggtgaac ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct
     7021 ggaagttgac ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt
     7081 ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgttgg
     7141 cggcgatcgc ctgctggaca tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt
     7201 ggcggtagct accattggtg ctgttctgcc gggtgatttt aaaattaaag cggcgaaact
     7261 gcgtggtgaa ccgtctgaag ggatgctgtg ctccttttct gagctgggaa tttctgacga
     7321 tcatagcggc attatcgagc tgcctgcgga tgcgccgatt ggcaccgaca tccgtgaata
     7381 cctgaaactt gatgacaaca ccatcgaaat cagcgtgacg ccaaaccgtg ccgactgttt
     7441 aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc
     7501 ggaaatcgtt ccggttggtg cgaccatcga cgacacgctg ccgattacag tcgaagcgcc
     7561 ggaagcctgc ccgcgttatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac
     7621 tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt
     7681 tgacgtcacc aactatgtac tgctcgaact gggccagccg atgcacgctt tcgataaaga
     7741 tcgcattgaa ggcggcattg tggtgcggat ggcgaaagag ggcgaaacgt tggtactgct
     7801 tgatggcact gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc
     7861 gctggcgatg ggcggcatct ttggtggcga acactctggc gtgaatgacg aaacgcaaaa
     7921 cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca
     7981 tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagca
     8041 caaagcgatg gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc
     8101 ggtaattgat atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg
     8161 tagcaaactg gatcgcctga tcggccatca tattgcggat gagcaggtaa ctgacattct
     8221 gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag
     8281 ctggcgtttc gatatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg
     8341 ctacaacaac atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga
     8401 agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt
     8461 gatcacctac agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc
     8521 cttactgctg ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac
     8581 cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt
     8641 cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct
     8701 gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga
     8761 gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tggccggtaa
     8821 actgaatgag gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc
     8881 agcgatttat ctgaaaggtg aacgtattgg ttttgttggg gttgttcatc ctgaactgga
     8941 acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc
     9001 agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat
     9061 cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt
     9121 tggcgtaaat caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc
     9181 ggaggggtat aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga
     9241 agaagaggag attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca
     9301 ggcatcattg agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt
     9361 ttgataagct tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag
     9421 agatccgtcg cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg
     9481 atctgcgtga taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca
     9541 ttacagcacg gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa
     9601 acgcttcgcc caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt
     9661 tcttttcact gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat
     9721 accatgctga cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg
     9781 tcagttttga tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatctcg
     9841 ccaggtgact ggttttctcc tcgtggcgaa ctgttcgtct ggcagattcg cctgccacgt
     9901 acgctggctg tattgctggt tggtgcggcg ctggctatat ccggcgcagt aatgcaggcg
     9961 ttgtttgaaa atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgta
    10021 gggcttatcg ccgcggtatt gcttgggcaa gggcaactcc caaactgggc actagggctg
    10081 tgtgcgattg ctggcgcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat
    10141 ctttcgacca gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta
    10201 atgacgtggg ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg
    10261 atgggcggtt ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc
    10321 gtgttgttgt ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc
    10381 tcggcgcggc aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc
    10441 ggctggatgg ttggcgtcag tgtggcgctg gcgggtgcta tcggctttat tggtctggtg
    10501 atcccacata ttcttcggtt gtgtggttta accgatcatc gcgtattact tcccggctgc
    10561 gcgctggcag gggcgagcgc attgctgctg gccgatgttg tagcgcgctt ggcattagct
    10621 gccgcagagc tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg
    10681 ttattgttaa aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt
    10741 tttactgacc acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag
    10801 atatcgacgg tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca
    10861 atgtcgcctc aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag
    10921 cctgggccga tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag
    10981 aaccgggcag cgatgaagag attaaaactt actgtaccac cacatggggg gtgacgttcc
    11041 cgatgttcag taagattgaa gttaatggcg aaggacgcca tccgctgtat caaaaattga
    11101 ttgccgcagc gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca
    11161 gcaaaggtcg tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag
    11221 ttggcaggga cggattggtc atccagcgtt tttccccgga tatgacgccg gaagacccca
    11281 ttgtgatgga aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca
    11341 ggatgttgcg gaatctaccc gcctggggcc actttctggc gaggttcggg ctggggagat
    11401 cctgcacctg gtggggccga atggcgcggg taagagtacc ttgcttgcac gaatggccgg
    11461 aatgaccagc ggtaagggaa gcattcagtt cgcggggcaa cccctggaag catggtccgc
    11521 aacaaaactc gcgctgcatc gcgcctatct ttcacaacag cagacgccgc cgtttgcaat
    11581 gccggtctgg cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa
    11641 tgatgtcgca ggggcgctgg ctcttgatga caaactcgga cgtagcacca atcaactttc
    11701 cggcggtgaa tggcaacgcg tacgtcttgc tgcggtggtg ctgcaaatca caccacaagc
    11761 caatcccgca ggtcaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca
    11821 acaaagtgcg ttagacaaaa ttctgagcgc tctgtgtcag caaggactgg cgattgtgat
    11881 gagcagtcac gatctcaacc acacattgcg tcatgcgcat cgggcgtggt tgctaaaagg
    11941 tggaaaaatg ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca
    12001 ggcctatggg atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac
    12061 catctgattt cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa
    12121 taaaaaacag aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt
    12181 ctggccgggt gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt
    12241 accgttattg ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat
    12301 ggtggcatga cgcggcgcgg tgtggactgt tcgggatttg tggttgtaac gatgcgcgat
    12361 cgtttcgatt tgcagctgcc ccgagaaacc aaagaacaag cctctatcgg cacgcaaatt
    12421 gataaagacg agttgctgcc tggtgacctg gtctttttca aaacgggttc cggacaaaat
    12481 ggtttgcatg taggtattta cgataccaac aaccaattta tccacgcctc taccagcaag
    12541 ggagtgatgc gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga
    12601 cgaatttagt atgtcgaaaa atggacggcg atgatacatc gccgtccatt tactcctgaa
    12661 tacagtagta ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata
    12721 atctaaaaaa aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactcctta
    12781 tgtaattgtt atgtcttatg gcctcatccc ttttagccgg atacagaaaa acatccttcg
    12841 agagggacgg ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc
    12901 ctgccgataa gagataacca gcaagacctt gtcggcgttg agttaattac ccatttctcc
    12961 agtgaagatg gtacggttcg tatcccgacg agtcgggtta tagcgcaact taccgaggag
    13021 caacattggc aattattttc agagcagttg gaattactaa aatcatgcca acattttttt
    13081 attcaacata aactctttgc ctggcttaat ttaacgccac aagtggctac attattatta
    13141 gagagggata attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat
    13201 gagaattacc cgcatctcaa tgaaggaaaa gataatcgcg atcttttatc cttatcgcaa
    13261 atgtatcccc ttgtgttagg caatttggga gcaggtaata gcacaatgaa agcggttttt
    13321 gatggtcttt tcacccgcgt catgctggat aaaagtttta ttcagcaaca aatcacgcat
    13381 cgctccttcg aaccgtttat tcgtgcgatc caggcgcaaa tttccccttg ttgcaattgc
    13441 atcatcgcgg ggggcattga tacgacagaa atattggcac agattacccc cttcgatttc
    13501 catgctctcc agggatgcct gtggcctgcc gttcctataa atcagataac gacgttggtt
    13561 cagcgataac cctcctgttt gctggtgttt aagacgagag taaccgtcta cactattaaa
    13621 caggaggagc tatgaccctg tcttttatta cccgctggcg cgatgaattg ccagcaacct
    13681 atacagcact ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac
    13741 tcgctaacac gctgagtatt ccatcgtcgc tgtttaaaaa tggcgcaggt gtctggggcg
    13801 gcgaaaccct actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg
    13861 gcgtctgggc gggccaactg ggtgatgggc gcggtatttt actcggcgaa caactgcttg
    13921 ctgatggcac tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa
    13981 tgggtgatgg acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga
    14041 tgcattatct gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag
    14101 tgtatcggga aacggtggag ccaggcgcga tgctgatgcg tgtggcacca agtcatctgc
    14161 gctttggtca tttcgaacat ttttactatc gccgcgagcc ggaaaaggtt cgtcagttag
    14221 ctgactttgc cattcgtcat tactggtcat atcttgaaga tgatgaggac aaataccgcc
    14281 tctggtttag cgatgttgtc gcacgtaccg catcgttaat tgcccaatgg cagacggtcg
    14341 gctttgctca tggggtgatg aataccgata acatgtcgct gctggggctg acgcttgatt
    14401 acgggccgtt tggttttctg gatgattacg aacccggttt tatttgtaat cactcggatc
    14461 atcaagggcg ttatagcttt gataatcaac ctgctgtcgc gttgtggaat ttacatcgtc
    14521 tggcgcagac attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct
    14581 atcagcaggt tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatga
    14641 cggagcaaaa agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc
    14701 gcagcgatta tacccgcaca ttccgcatgc tgagtctgac cgagcagcac agcgcggcgt
    14761 caccgctacg tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc
    14821 gggggcgttt gcaacaagac gaggttagcg atagtgagcg tcagcagctg atgcaaagcg
    14881 ttaacccagc tctggtgttg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa
    14941 agggtgatat gacggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca
    15001 gagctgatga ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct
    15061 cgagttaaag gctacttggt caacagcagc ttgccagcct gagttttacg gagcagatat
    15121 tcttgcccgt catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggaa
    15181 attcgccgaa taggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtga
//
LOCUS       DAMZII010000108        15217 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZII010000108 DAMZII010000000
VERSION     DAMZII010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.108"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            182..1813
                     /gene="oppA"
                     /locus_tag="POA77_003477"
     CDS             182..1813
                     /gene="oppA"
                     /locus_tag="POA77_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDI6366848.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1899..2819
                     /gene="oppB"
                     /locus_tag="POA77_003478"
     CDS             1899..2819
                     /gene="oppB"
                     /locus_tag="POA77_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDI6366849.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2834..3742
                     /gene="oppC"
                     /locus_tag="POA77_003479"
     CDS             2834..3742
                     /gene="oppC"
                     /locus_tag="POA77_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDI6366850.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3754..4767
                     /gene="oppD"
                     /locus_tag="POA77_003480"
     CDS             3754..4767
                     /gene="oppD"
                     /locus_tag="POA77_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDI6366851.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4764..5768
                     /gene="oppF"
                     /locus_tag="POA77_003481"
     CDS             4764..5768
                     /gene="oppF"
                     /locus_tag="POA77_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDI6366852.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5821..6150)
                     /locus_tag="POA77_003482"
     CDS             complement(5821..6150)
                     /locus_tag="POA77_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDI6366853.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6185..7645)
                     /gene="cls"
                     /locus_tag="POA77_003483"
     CDS             complement(6185..7645)
                     /gene="cls"
                     /locus_tag="POA77_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6366854.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7788..7961
                     /locus_tag="POA77_003484"
     CDS             7788..7961
                     /locus_tag="POA77_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDI6366855.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8016..9269)
                     /gene="kch"
                     /locus_tag="POA77_003485"
     CDS             complement(8016..9269)
                     /gene="kch"
                     /locus_tag="POA77_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDI6366856.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9568..9864)
                     /locus_tag="POA77_003486"
     CDS             complement(9568..9864)
                     /locus_tag="POA77_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDI6366857.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10088..10807
                     /gene="tonB"
                     /locus_tag="POA77_003487"
     CDS             10088..10807
                     /gene="tonB"
                     /locus_tag="POA77_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDI6366858.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(10847..11245)
                     /gene="yciA"
                     /locus_tag="POA77_003488"
     CDS             complement(10847..11245)
                     /gene="yciA"
                     /locus_tag="POA77_003488"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDI6366859.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11350..11889)
                     /locus_tag="POA77_003489"
     CDS             complement(11350..11889)
                     /locus_tag="POA77_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDI6366860.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(11919..12662)
                     /locus_tag="POA77_003490"
     CDS             complement(11919..12662)
                     /locus_tag="POA77_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDI6366861.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12690..12779)
                     /locus_tag="POA77_003491"
                     /pseudo
     CDS             complement(12690..12779)
                     /locus_tag="POA77_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13019..13657
                     /gene="ompW"
                     /locus_tag="POA77_003492"
     CDS             13019..13657
                     /gene="ompW"
                     /locus_tag="POA77_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDI6366862.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13717..14223)
                     /locus_tag="POA77_003493"
     CDS             complement(13717..14223)
                     /locus_tag="POA77_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDI6366863.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14269..14769)
                     /locus_tag="POA77_003494"
     CDS             complement(14269..14769)
                     /locus_tag="POA77_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDI6366864.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(14855..15034)
                     /locus_tag="POA77_003495"
     CDS             complement(14855..15034)
                     /locus_tag="POA77_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6366865.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4038 a         3572 c         3678 g         3929 t
ORIGIN      
        1 cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa agtcggtgat
       61 agtaatacgt aacgataaag taacctgaca gcagaaagtc tccgagcctg tgcagggtcc
      121 caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag tccaaaaaac
      181 aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg cgctaatggc
      241 agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg aaaaacaaac
      301 actggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa ttgaaggtgt
      361 tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg atcttgacgg
      421 tcatccagca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag tctggacctt
      481 ccatttgcgt aaagatgcga aatggtctga tggcacgcca gtcacagcac aagactttgt
      541 gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca gttatctgca
      601 atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga ttaccgatct
      661 cggcgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac ccgttccgta
      721 cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg ctatcgagaa
      781 attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct ataccttaaa
      841 agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact ggaacaacgc
      901 gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta ccgatgtcaa
      961 ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg aattgttcca
     1021 gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt gcacttacta
     1081 ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta ccgcactgaa
     1141 actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg acatgcccgc
     1201 ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg aatggtttgg
     1261 ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag cgggttatac
     1321 cgcagacaaa ccgttgacca tcaacctgtt gtataacacc tccgatctgc ataaaaagct
     1381 ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca aactggttaa
     1441 ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg tggcccgtgc
     1501 aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc tttcgaacag
     1561 ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg cggaaacgct
     1621 gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac aacagctgga
     1681 taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg tgaaaccgtg
     1741 ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga atatgtacat
     1801 tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt gtctgatttt
     1861 tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt attctacgtc
     1921 gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc tttatgatgc
     1981 gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgcctgaa gtgatggcca
     2041 atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc agctacctga
     2101 aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat tcggtcaatg
     2161 acctggtggc atccagtttt cccgtttctg ccaaactggg agccgcagca tttttccttg
     2221 cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac accaaatggg
     2281 actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt gtggttgcgc
     2341 cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt ggctggaatg
     2401 gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgcc tatatcgcca
     2461 gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat tttattcgta
     2521 ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgccacgca ttaaaacctg
     2581 ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt cggcattatt accggttcta
     2641 tggttatcga aaccatttat ggtttgccgg ggattgggca actgttcgtt aatggcgcat
     2701 tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct ttaaccattt
     2761 tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc cgttactgat
     2821 actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa atttcagtga
     2881 aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt ttatgcataa
     2941 ccgtgcggcg gttgccagtc tgatagtgct ggtgctgatc gcgttatttg taatcctggc
     3001 accgatgctt tcgcagtttg cctatgacga tactgactgg gcgatgatgt ccagcgcccc
     3061 ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc tgcttgtgcg
     3121 cgttgcgatt ggcgggcgta tctcactcat ggtcggtgtt gctgcggcac tggtggcagt
     3181 ggtcgtcggg acactttatg gttcgctttc cggttatctg ggcggtaaag tggattcggt
     3241 aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg tcattttgct
     3301 ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca tggtttcctg
     3361 gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca aagagtttat
     3421 tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc acattgtgcc
     3481 gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca tgatcctctt
     3541 tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca gctggggtgc
     3601 attgctgagt gatggcgcga actcgatgga agtctctcca tggttattgt tgttcccagc
     3661 gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct tgcgtgatgc
     3721 cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac tgcaactgtg
     3781 ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg tgtcaccttt
     3841 agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct acgtgccgga
     3901 gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc atttgcgttg
     3961 atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa tgggcgtgaa
     4021 atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat ctcaatgatt
     4081 tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca gttaatggaa
     4141 gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc ggtgcggatg
     4201 ctcgatgcgg taaaaatgcc ggaagcgcgt aaacgcatga aaatgtaccc gcacgaattt
     4261 tctggcggca tgcgtcagcg agtcatgatt gcgatggcat tgctatgtcg acctaagcta
     4321 ctgattgcgg atgaacccac tactgcgctg gacgtcaccg tacaggcgca gatcatgacg
     4381 ctattaaatg aactgaagcg ggaatttaat accgccatca ttatgatcac ccacgatctt
     4441 ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg cacgatggaa
     4501 tatggcaatg cgcgcgatgt cttttatcaa cccgttcatc cttattctat cggtttgctc
     4561 aacgcggtgc cgcgtcttga tgcggaaggt gaaacaatgt tgaccatccc gggtaatccg
     4621 ccaaacttgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc acatgcgatg
     4681 gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ctggccgtct gcgtgcttgc
     4741 tttaaaccgg tggaggaact gctatgaatg ctgtaactga aggaagaaaa gtcctccttg
     4801 aaatcgccga tcttaaagtg cactttgaaa tcaaagatgg caaacagtgg ttctggcaac
     4861 cgccgaaaac gctcaaagcc gtcgatggtg tcactcttcg cctgtatgaa ggggaaacat
     4921 taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc atcatcggtt
     4981 tggtcaaggc gaccgacggt catgttgcct ggttaggtaa agagttgctg ggcatgaagc
     5041 ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat ccgttggcat
     5101 cgctaaaccc gcgtatgacc atcggcgaga tcatcgctga accactgcgt acttatcatc
     5161 cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc gatgatgctg aaagtcgggt
     5221 tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag tgccagcgta
     5281 tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgcgat gagccggttt
     5341 cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag ctgcaacgtg
     5401 agatgggatt gtcattaatt tttatcgctc atgacctggc cgtggtaaaa cacatttccg
     5461 atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat gatgaggtct
     5521 accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc atacctgatc
     5581 cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg tcgccgatca
     5641 acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg gagtgcgcca
     5701 aaacacgtcc tgttctggag gggagtttca gacacgccgt ttcttgcctg aaagtcgatc
     5761 cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgctaa aagcgattat
     5821 ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata acagaatgcg
     5881 ggcgaaaaca tcgttgattt caccatcttc actgtccgcc agaccaatca ccacttcggc
     5941 aaaaaagtct gggttcaaat cgaaatcaac atgttcctgc cagtcttccg ccggatcgaa
     6001 taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat cagctggatc
     6061 gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg tttcatcttc
     6121 agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct ctgttggcga
     6181 cgttttacag caacggactg aagaagtaaa acagtcgctc ggcgacacgt tgccataatg
     6241 gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa tcgtcctgaa
     6301 cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc tcgaaattca
     6361 gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg ccatcgacca
     6421 gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc ccagcagcaa
     6481 gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca ttttttcgcg
     6541 gaaggataat actgacatcc accccgcgct gcgccgccgt gcaaatcgca tgaagtaaat
     6601 catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc gaataagccg
     6661 cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa gcaattgtgt
     6721 gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt ggtggcggca
     6781 gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc atcgcggtgg
     6841 cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc gcatcttgtt
     6901 tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa ttatcgatca
     6961 tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc atcagattga
     7021 cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc cacgggctgc
     7081 ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaata ccacgccgtg
     7141 cagccgccat taatgattca gccacctggt ccgccatgcc gccgggctgc cagatataaa
     7201 acaccatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc tgcatcacat
     7261 catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc ccctgacgac
     7321 gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg aagatatgct
     7381 tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct ctggcgcgct
     7441 cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata attccgacta
     7501 acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg cgtcgtttca
     7561 ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg gccaaccaac
     7621 tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc attgttatga
     7681 gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg gtcagcgtcg
     7741 ggggtggggg tataatgacc attctgttat tgcatagagt agttaacatg aagcggagta
     7801 gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa tcgcgttggc
     7861 ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc attctaaaca
     7921 aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatatactgg gcaccattgc
     7981 ggtgcccaat aagtttactc tttcgtcttt aaactctatt tttgcactga ttctttactg
     8041 tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga gttcaacaac
     8101 attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc cagaatttcg
     8161 ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat cttattcagg
     8221 tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc ttttgccgag
     8281 agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc ccggcagcga
     8341 tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat aacatcagcg
     8401 ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt gctgataacc
     8461 gttacgtttt gtccgcgttg attcagttgc aggatcgtat tgatggcgag aatcgaatgt
     8521 ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt tacaagtttg
     8581 ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc aaaaacggta
     8641 atgccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac agggacaata
     8701 tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt catcaaactt
     8761 tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga gtaaaacagt
     8821 aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact gtgggagaag
     8881 tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat actaaatttc
     8941 agccagggat aaaaatgcag ggtgtaaatt agcgctatca acaacaaaat gatactgatt
     9001 gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag aaagacgccg
     9061 agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa tgctttgata
     9121 tggaaaatat caagcaaatt gaccgacata ccatagattg ttttaaaaat aagcaatcca
     9181 tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa atttgttgct
     9241 gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat tttgtttaat
     9301 tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt aaacaatagt
     9361 agatgacttt atcggcagta atcaaaggtt ttgagcgagc aactctgcgt caggaaaggg
     9421 cattttcctg aatatgctca catcatataa agaaatacag ataaagttat tatctgcttg
     9481 tggtagtgaa tgcactgacc ggctataagg aaaggccaaa caagaacacg gttgcaaaaa
     9541 ccgtgccctt aaatattgaa tctctattca gaacactttc ttaaatggtt tcactgaaac
     9601 gtgttcatag actcctgccg ctacgtacgg gtcagcatcg gcccaggcct gagctgcttc
     9661 cagcgattca aattcagcaa taacggttga gccagtaaat cccgcagccc ctggatcgtt
     9721 actgtctacc gctggcattg gaccagctgt caacaaacga ccttcatcat gcagtaactg
     9781 taaacgtgct aaatgtgccg gacgaacgga aaggcgtttt tcgaggctat cagctttgtc
     9841 ttgagcgtaa ataacataca acacgggcac aactccttgt tcgggaaagt tgtaaagtac
     9901 gttatttgaa agggcgaaga tctgcaacgg aaagatgatg tctttgttaa gaccatgcat
     9961 aaattaaggt caattacgcc aaaaatgaca ttttcactga ttctgatcgt cttgccttat
    10021 tgaatatgat tgctatttgc atttaaaatc gagacctggt ttttctactg aaatgattat
    10081 gacttcaatg acccttgatt tacctcgccg cttcccctgg ccgacgttac tttcggtctg
    10141 cattcatggt gctgttgtgg cgggtctgct ctatacctcg gtacatcagg ttattgaact
    10201 acctgcgcct gcgcagccaa tttctgtcac gatggttgcg cctgctgatc tcgaaccgcc
    10261 acaagccgtt cagccgccac cggagccggt ggtagagcca gaaccggaac ctgagccgat
    10321 ccccgaaccg ccaaaagaag caccggtggt cattgaaaag ccgaagccga aacctaagcc
    10381 aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa cgcgatatca aacccgtaga
    10441 gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgcg cgccccacat caagtacggc
    10501 aacggctgca accagcaagc cggttaccag tgtggcttca ggaccacgcg cattaagccg
    10561 taatcagccg cagtatccgg cacgagcaca ggcattgcgc attgaagggc aggttaaagt
    10621 taaatttgac gtcacgccgg atggtcgcgt ggataacgta caaatcctct cagccaagcc
    10681 tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc agatggcgtt atgagccggg
    10741 taagccaggc agtgggattg tggtgaatat cctgtttaaa attaacggca ccaccgaaat
    10801 tcagtaagca gaaagtcaaa agcctccgac cggaggcttt tgactattac tcaacaggta
    10861 aggcgcgagg ttttccttca ggatcaaccg cgacatactt aaataatgct tctgtcgctt
    10921 tatagcgttg cccgattggt tcagacgcta cttttttcac ccacacttca atattaatgc
    10981 tgaccgatgt cgtccctttc tggacacagc gtgcatagca gcacaccaca tcgccgaccg
    11041 caaccggccg taagaaagtc attccttcaa cccgcacagt cactacgcga ccgtgggcaa
    11101 tttcttttgc cagaatagcg ccgccaatat ccatttgtga cattaaccaa ccaccaaaga
    11161 tgtcaccatt ggcattggta tcggcgggca tggctaaagt acgtaaaaca agatcgccct
    11221 gagggacgtt atgtgttgta gacatggtaa aaccgactta aaaggaaaat cacaggcgcg
    11281 atgctactat gattttcctg aggagaacag aggagataca gcgccagccc cgaaggactg
    11341 gcagtctggt taggatttat cttcctgcgg catgtggcgg tagatataga taccgcttaa
    11401 caatgtgaag attaaggtaa gggcggtcag gccaaagact ttaaagttga cccaaatatt
    11461 ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg caaaggataa agaaaacagc
    11521 ccaggccaga ttcagcttcg accataccgg ttgcggcagc gtgagttctt tacccagcat
    11581 ccgctgaatt agcggctttt tcatcaccca ttggctgact aacagggctc ccgcaaatag
    11641 agcataaatg actgtaacct tccatttaat aaactcatca ttgtggaaga acagcgtcaa
    11701 gccaccgaag acgaccacca gaacaaaagt gatcagggcc atcttctcaa ccttacgaaa
    11761 gcgaacccag ctatagataa gcacaatcgc cgtggcgacg atcagcgccg cagtagccgc
    11821 atagatgtca taaatcttgt aaaacgcgaa aaagacaacc agcggtaaaa aatcaagaaa
    11881 ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt attggcgaat caacatatac
    11941 aggcggaaca gatagatgag caatatggca gaaatcaggt tgctcaaggt gttcgccaga
    12001 acggcaccaa tttccggggt taatgcggca aaagaagagg caaaaagcag caacagtgtt
    12061 tttgccagca accagctcag tactgcgggt gccaccagac gcatattcgc ccaagtcagc
    12121 cgcatactgc tacgcatcga ggcaaaaatg cccattttgt cctgtaccag catcaccggt
    12181 gccagggcca gtaaaatggc catgataatt cccggaacga ccaccagcat gatgccaatc
    12241 tgtactaaaa gggtagtcag aaaaatcaga ataaataact tgggcaatat cggcgcactg
    12301 gcaccaatag ctcgcagcgc actgactctc tgacccgcag acaccagctg gataattaat
    12361 attacgcctc cggcgagaat ggcgttaccg attaatcctg aaaaagtgga cgccgctgaa
    12421 gcctgcagca aaatttgttg ctgttccggt gacatattct gaaccaggtc gaacaaccca
    12481 ctactgccgc taacgggcac gccgtcattg agctgcgcaa gctgtgcatc actgggtgag
    12541 aaaacatgcc ctaacaccac tgtgataaac gcacatagca acgataccaa cagaatggtc
    12601 ataaattgat tacggaagaa atttccggtg tcacggtata cggactgcgc cgtgatagac
    12661 atgcactctc cttgagtttt atgcaggtgt taattagcgg gcaattgtac cctggttaag
    12721 cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat ctttgtaaag cggaggtaat
    12781 tgctggcctt caggcgtggg caaaccgcgg caagtggcga tcgagttaat atttgcgtag
    12841 agaaaatatt taaaaattga tttaaatcac attaaccagg attctcaatg caacttctaa
    12901 attaatccag atcaataaat ggtgaattat catatgtaat gtgatctatg taggatcatt
    12961 tgttactcca atgtaggtat attcgtcacg tttttataac cataacgacg gagcggatat
    13021 gaaaaagtta acagtggcgg ctttggcagt aacaactctt ctttctggca gtgcctttgc
    13081 gcatgaagca ggcgaatttt ttatgcgtgc aggttctgca accgtacgtc caacagaagg
    13141 tgctggtggt acgttaggaa gtctgggtgg attcagcgtg accaataaca ctcaactggg
    13201 cttgacgttt acttatatgg cgaccgacaa cattggtgtg gaattactgg cagcgacgcc
    13261 gttccgccat aaaatcggca cccgggcgac cggcgatatt gcaaccgttc atcatctgcc
    13321 gccaacactg atggcgcagt ggtattttgg tgatgccagc agcaaattcc gtccttacgt
    13381 tggggccggt attaactaca ccaccttctt tgataatgga tttaacgatc atggcaaaga
    13441 ggcagggctt tccgatctca gtctgaaaga ttcctgggga gctgccgggc aggtgggggt
    13501 tgattatctg attaaccgtg actggttggt taacatgtca gtctggtaca tggatatcga
    13561 taccaccgcc aattataagc taggcggtgc acagcaacac gatagcgtac gcctcgatcc
    13621 gtgggtgttt atgttctcag caggatatcg tttttaattc cacaaaaaaa cgaccccgta
    13681 atatacgggg tcaataagga catggcataa agtgtattat ttcttcgctt ctacgccgtc
    13741 agtttcagag cgaattaaaa atttctcagt tgtttgcgga atatgctgaa tcagccagtc
    13801 ggccatttgc ttttcctcat ttaaaatcgc ttcgatggtt ggaattgaag ctgtatcacc
    13861 ggcatttttt gctgctgcta atagtgaggt gtaacaggcg atttcaaatt gctcgaagac
    13921 atatccgcta atagagcctt tgactatttc atcagaaggg aatataccac cgattgactg
    13981 cccaagcgca gccattttac tcatggaatc tttaatgact gaacgtgaaa tgtcattacg
    14041 atcaagaata gtttccaatt gaacaatctg gtttttggtt tcactaagat gttgttcaat
    14101 acgagcgcgt agttcaggat aattatctat acggctggcc atggattcaa gcatagattc
    14161 ggcttgcttt tccattgcgt gggcgtcacg taaccagtca tgataatgtt caatacgatt
    14221 catgaaaatg tcctctttat aaaatgacta aaagttaaag ttcatatttc aggctttatt
    14281 ttcggctttc ttatttacgt tattaagggc cagatcagtc agtttgatgt cggtggcctt
    14341 ttcttcttcc agggtctctt tcagaagctt cgctgcttta cgataaccta attgttcagc
    14401 cagcgtcgct aatgtcccgt aactggcaat ctcataatgc tcgactttct gtgctgcggc
    14461 aatcagtgcg gcatcacgca cttcgttttt ctcggtactt tcgatgacct cattagcttc
    14521 ttcaataaga ccttccattg ccacacattt catgcgctta attttcagat tcgattctga
    14581 ttccacaact tgatcaatac gttcaatctg tccatgtgtt tcctcgaggt gcgcatgaaa
    14641 agcctgactt aatttttcgt ttgatgttgc tcttgcgagt tttgccagtg cccgggttaa
    14701 ttgtttttct gcgctgtagg tatctgaaag caggtgaata aatacatctt caatggtctt
    14761 catattcata tttttctcca gtgaaatcac tgcgagccat acggcccgca gatgcaatta
    14821 tctttatcag ctgatattaa taattaacgg tgaatcagga tttgcctgat ttattaccac
    14881 cgctttgttg accgcctttt ttacccgctt cagatgcgcg ttgcggatca tttttaaaat
    14941 taccgccgct atgctgaccg cctttacggc ctgcgtcgga tgccttctca cggtcttcgg
    15001 cgaaatttcc tgaaccacca cgatgttcgg ccatgttatt tctcccgttg cgttgcattg
    15061 tttcattaat atgagtgttg tgtgtcgaca ctcattaaaa ttagtcgcta atgagaatta
    15121 gtcaaattaa gcgcaacgag aagatagagg gaaaatatat tttgaggaac attctggata
    15181 tattaacaat tacccgagga ataagtgact tagagaa
//
LOCUS       DAMZII010000109        15003 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZII010000109 DAMZII010000000
VERSION     DAMZII010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.109"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(44..2323)
                     /gene="maeB"
                     /locus_tag="POA77_003496"
     CDS             complement(44..2323)
                     /gene="maeB"
                     /locus_tag="POA77_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDI6366866.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2612..3562
                     /gene="tal"
                     /locus_tag="POA77_003497"
     CDS             2612..3562
                     /gene="tal"
                     /locus_tag="POA77_003497"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6366867.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3582..5585
                     /gene="tkt"
                     /locus_tag="POA77_003498"
     CDS             3582..5585
                     /gene="tkt"
                     /locus_tag="POA77_003498"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6366868.1"
                     /translation="MSRKDLANAIRALSMDAVQKAHSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5681..6724)
                     /locus_tag="POA77_003499"
     CDS             complement(5681..6724)
                     /locus_tag="POA77_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDI6366869.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITEFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6850..7425)
                     /gene="nudK"
                     /locus_tag="POA77_003500"
     CDS             complement(6850..7425)
                     /gene="nudK"
                     /locus_tag="POA77_003500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDI6366870.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7493..9472)
                     /gene="aegA"
                     /locus_tag="POA77_003501"
     CDS             complement(7493..9472)
                     /gene="aegA"
                     /locus_tag="POA77_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDI6366871.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9678..11378
                     /gene="narQ"
                     /locus_tag="POA77_003502"
     CDS             9678..11378
                     /gene="narQ"
                     /locus_tag="POA77_003502"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDI6366872.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11542..14655
                     /gene="acrD"
                     /locus_tag="POA77_003503"
     CDS             11542..14655
                     /gene="acrD"
                     /locus_tag="POA77_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDI6366873.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14754..14813)
                     /gene="ypfM"
                     /locus_tag="POA77_003504"
     CDS             complement(14754..14813)
                     /gene="ypfM"
                     /locus_tag="POA77_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDI6366874.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3508 a         3988 c         3921 g         3586 t
ORIGIN      
        1 cttaccctga atattcaggg taagcgtgag agttaaaaaa gaattacagc ggttgggttt
       61 gcgcttcaac cacggccagc gccaccatgt tgacgatacg acgcaccgat gcgatcggcg
      121 ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg
      181 agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
      241 gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca
      301 ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga
      361 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
      421 agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca
      481 tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
      541 taaaggtgtt accactcggc agcagcagcg cgttcattgc acctgcggtg tgaacgccat
      601 cgcgataacc aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac
      661 cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt
      721 tactgatcag cgcccgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
      781 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atctcaaaat
      841 caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
      901 gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtg gcatgcagaa
      961 cgcgcgcctc ttccccttcc ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga
     1021 aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga
     1081 cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgccgct ttcgccaccg
     1141 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
     1201 caaagctcag atcctgatcg ccatacgctg aagccaccac ttcgctctgt tccgcatggg
     1261 ccagttctgc aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc
     1321 caacgtccag cgcgccacgg aagatgaacg ggaagcaaag gacgttgttg acctggtttg
     1381 gatagtcaga acgaccggtg cagatgatgg catccggacg cacttctttc gccagcggcg
     1441 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggcgcacga gccattttct
     1501 tcaccatttc ctgggtcagc actttcgggc cggaacatcc gaggaaaata tccgcgcctt
     1561 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
     1621 ccgccatgtt tggctcacgg ccctgataga taacgccttt tgaatcgcaa accacgatgt
     1681 tatgtttttg cagacccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
     1741 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga
     1801 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
     1861 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
     1921 ggttgattcc gccgaaggtc ggttcgagcg cagcgacaac ttcaataaat ttgtccgggt
     1981 cgagttcatc aacttcaatg tcaaatacat caatcccggc gaatttctta aacagaacgc
     2041 ccttgccttc catcaccggt ttgccagcca gcgcgccaat gttgcctaac cccagcaccg
     2101 ccgtaccgtt agagatcacc gccaccaggt taccgcgcgc ggtatatttg taggctttta
     2161 acgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
     2221 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
     2281 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
     2341 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
     2401 ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
     2461 tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc
     2521 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
     2581 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
     2641 caccactgtc gtggcagaca gcggcgatat tgagtccatt cgccattatc atccccagga
     2701 tgccaccacc aatccttcgc tgttactcaa agctgccgga ttatcacaat atgagcattt
     2761 aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt
     2821 cgcagcgtgt gacaaactgg cagtcaattt cggtgctgaa attctcaaaa tcgtacccgg
     2881 tcgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa
     2941 ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat
     3001 caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat
     3061 taactgcaac ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg cagaagcggg
     3121 cgtttttctg atttcgccgt ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc
     3181 gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga
     3241 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
     3301 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
     3361 gctgcaggaa aaagtttcgc cagtggtacg taaattaatc ccgccttctc agacgttccc
     3421 acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
     3481 ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga
     3541 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
     3601 ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa agcccattct ggtcatcccg
     3661 gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata
     3721 atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
     3781 cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga
     3841 agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag
     3901 gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga
     3961 tagcggaacg tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact
     4021 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
     4081 cgctggcagg cacgctggga ctgggcaagc tgattggttt ttacgatcac aacggtattt
     4141 ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
     4201 atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga
     4261 tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg
     4321 gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
     4381 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta
     4441 aaaagattta tcacgcctgg gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga
     4501 atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgac
     4561 ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
     4621 tacaggcaaa tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt
     4681 acgggccgat gctgcctgag ttgctcggcg gttcggcgga tctggctccc agcaacctga
     4741 ccatctggaa aggttctgtt tcgctgaagg aagatccagc gggcaactac attcactacg
     4801 gggtgcgtga atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg
     4861 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg
     4921 cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg
     4981 aagatggtcc gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt
     5041 tcagcacctg gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg
     5101 agcgccacaa cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac
     5161 gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg
     5221 gtaagccaga tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg
     5281 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata
     5341 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
     5401 gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg
     5461 caattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct
     5521 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
     5581 cctgatggtg atcgccggat gctgattgcc ggatgcgacg ctgacgcgtc ttatccggcc
     5641 tacagcgtct tatccggcct acatgtcccc gcaattttgt ttaacgggtg atccacaacg
     5701 tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggca taacgcacca
     5761 ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc
     5821 gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt
     5881 ccagttcatt cgtctcctga ccgttgttga acggcaaacg caaccgaacc gggcgcgagg
     5941 ctagtggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct
     6001 cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac
     6061 gtaacggatc aagcgagcag cgtagaccat tcatccgcca gtttccataa tccagcaaat
     6121 cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccacg acctctttca
     6181 gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc cacgcggttt
     6241 cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccactca
     6301 gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcatctgca
     6361 aaaactcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact
     6421 tgtcaccact taacgccaac ggctcaccat ctaacagcag ccgtggcgct atctcccctt
     6481 cagacgcatc cggcgatttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg
     6541 ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta ttacgcgcca
     6601 cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg
     6661 ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc
     6721 gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc
     6781 aggtagcgcg gctcaatcgg atttgtcgga ttgagccaac aataaacaac gaaatattat
     6841 tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata acaccgtctt
     6901 accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag
     6961 cacttcaata tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta
     7021 ttcggcgata aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa
     7081 taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca
     7141 cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc
     7201 attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg tctttttctt
     7261 cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg
     7321 gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg tgaaatagtt
     7381 atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg
     7441 cgaaatgaac aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag
     7501 atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg
     7561 ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg
     7621 gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt
     7681 ctaccgttac accgtgcgac tccagccacg gcatcccgtg tggattgaag ccaaacgcca
     7741 taatcaccgc atcggctggc atgacaaatt cactgccctc aatcggtaca ggtcgccgac
     7801 gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaattccg caaacgtgac
     7861 cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt
     7921 cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag
     7981 cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt
     8041 cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta
     8101 gctcttcgag ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa
     8161 cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa
     8221 cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac
     8281 aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg
     8341 atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccaatt tctggatggc
     8401 gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg
     8461 ccgggcctgc cccgataatc gccacccgct tgtccacttt ggtgacatga cttaagtcag
     8521 gacgccaacc tttcgccaac gcctgatctg aaatatagcg ttcaatgttg ccgatagtta
     8581 ccgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa
     8641 cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga
     8701 tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc gggcaggtcc
     8761 attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt tgtgcctggt
     8821 cggcgcgaaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt
     8881 tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca
     8941 tttcttgcgc cgcggtactg gcatgccacg gttgatgttc ctgacgcgcg gtgcgcaagc
     9001 ggcgggattt cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg
     9061 ggcaattctc aacacaggca ggaccgtttt cgcgccccgc acaaaggtcg catttatgcg
     9121 ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaaag
     9181 gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt
     9241 catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat
     9301 ggtgacaggt cactgcacta cgttgctgtt gatgtttgat aaccgtaatt cggggatgaa
     9361 aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac
     9421 aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct
     9481 tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag
     9541 gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag
     9601 atcaaataat cccatcactg actaaattgt gtttcagcga actggaacat taatgatttt
     9661 ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct
     9721 ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga
     9781 gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg cagagttacc
     9841 gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc
     9901 agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg ccagaagcag
     9961 taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca
    10021 agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt
    10081 tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc
    10141 tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgcgccgc atacgccatc
    10201 aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa cacggacagt
    10261 tcgactcgcc gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct
    10321 ttaaccagat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag
    10381 aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat cagtgttcgc
    10441 aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc
    10501 gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg
    10561 aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg
    10621 tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca
    10681 gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc
    10741 agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc
    10801 aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata
    10861 acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc
    10921 agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg
    10981 cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact
    11041 gccgtctgcc aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg caaattattc
    11101 gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg
    11161 tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt ggcatcggtg
    11221 aaccgaaaga gcccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc
    11281 tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct
    11341 ttcgctccgc ggagggtgag gaaagtcagc tgatgtaatg cctcctactg accaaagaat
    11401 acttgcactt aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct
    11461 ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca
    11521 gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg
    11581 gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttctcatt gcccgttgaa
    11641 caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg
    11701 gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat
    11761 aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt
    11821 tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca
    11881 gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc
    11941 gataccaata ttctgaccat tgctttcgtc tctaccgatg gttcgatgga taaacaggat
    12001 attgccgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg
    12061 gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc
    12121 aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag
    12181 attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc
    12241 attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg
    12301 aatcaagacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag
    12361 aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggctggg ggtaaaactg
    12421 gcctccggcg caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg
    12481 gcgcagtact tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt
    12541 aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc
    12601 gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg
    12661 gtggtgttga tgggaacctt ctccgtactt tacgcctttg gttacagcgt caacacctta
    12721 accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg
    12781 gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa
    12841 tcgatggggc agatccaggg ggcactggtc gggattgcga tggttctttc ggcggtattt
    12901 gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc
    12961 attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt
    13021 gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc
    13081 tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt
    13141 ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc
    13201 ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc
    13261 tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc
    13321 gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc cactgttggt
    13381 tctggccccg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct gaaagactgg
    13441 agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacaaaggcg
    13501 tttaacaaga ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt
    13561 ggtagttctg cgggttttga tatggagttg caggaccacg ctggagcagg tcacgatgcg
    13621 ctgatggcag cacgtaacca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt
    13681 gtgcgccata acggcctcga cgacagtccg cagttgcaga ttgatatcga ccagcgtaaa
    13741 gctcaggcgc tgggcgttga tatcgacgat attaacgaca cactgcaaac cgcctggggt
    13801 tcgagctatg ttaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca
    13861 gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg aaataaagat
    13921 ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg ctcgccgcgt
    13981 ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc accgggggtc
    14041 agtaccggta cggcgatgga tattatggaa tcgttagtga agcagttgcc aaacggcttt
    14101 ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg
    14161 ctgtacgcta tttccttgct ggtggtattc ctgtgtctgg cggcgttgta tgaaagctgg
    14221 tcggtgccgt tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc gctgctggca
    14281 acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt
    14341 ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa
    14401 ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg
    14461 atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt
    14521 tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc ggccactatt
    14581 ctggctattt acttcgtgcc gctgttcttt gtactggtgc gccgccgctt cccgctgaag
    14641 ccgcgcccgg aataagcaat aaaaaaggcg acatgctaat gtgtcgcctt tttcaacttt
    14701 ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag gaattactta
    14761 cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc
    14821 tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg
    14881 attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg tcctgcggtg
    14941 ataatttgta tgtgatacac agcaacattt cgagatattc atacggcatc taatactgat
    15001 tta
//
LOCUS       DAMZII010000110        15003 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZII010000110 DAMZII010000000
VERSION     DAMZII010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.110"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1324
                     /locus_tag="POA77_003505"
     CDS             <1..1324
                     /locus_tag="POA77_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDI6366875.1"
                     /translation="LSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQ
                     VAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYNDFYDIGRKNSLS
                     ANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENN
                     KEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLN
                     YGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGIN
                     ITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSES
                     ETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIG
                     VVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1337..2296
                     /locus_tag="POA77_003506"
     CDS             1337..2296
                     /locus_tag="POA77_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDI6366876.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSIRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2335..3234)
                     /gene="nhaR"
                     /locus_tag="POA77_003507"
     CDS             complement(2335..3234)
                     /gene="nhaR"
                     /locus_tag="POA77_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDI6366877.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3300..4466)
                     /gene="nhaA"
                     /locus_tag="POA77_003508"
     CDS             complement(3300..4466)
                     /gene="nhaA"
                     /locus_tag="POA77_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDI6366878.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5052..5204
                     /gene="mokC"
                     /locus_tag="POA77_003509"
     CDS             5052..5204
                     /gene="mokC"
                     /locus_tag="POA77_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDI6366879.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5308..6438)
                     /gene="dnaJ"
                     /locus_tag="POA77_003510"
     CDS             complement(5308..6438)
                     /gene="dnaJ"
                     /locus_tag="POA77_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI6366880.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6527..8443)
                     /gene="dnaK"
                     /locus_tag="POA77_003511"
     CDS             complement(6527..8443)
                     /gene="dnaK"
                     /locus_tag="POA77_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI6366881.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8820..9224
                     /locus_tag="POA77_003512"
     CDS             8820..9224
                     /locus_tag="POA77_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDI6366882.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            9250..9963
                     /gene="msyB"
                     /locus_tag="POA77_003513"
     CDS             9250..9963
                     /gene="msyB"
                     /locus_tag="POA77_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDI6366883.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10112..10678
                     /gene="satP"
                     /locus_tag="POA77_003514"
     CDS             10112..10678
                     /gene="satP"
                     /locus_tag="POA77_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDI6366884.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10713..11300)
                     /gene="mog"
                     /locus_tag="POA77_003515"
     CDS             complement(10713..11300)
                     /gene="mog"
                     /locus_tag="POA77_003515"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDI6366885.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(11415..12368)
                     /gene="tal"
                     /locus_tag="POA77_003516"
     CDS             complement(11415..12368)
                     /gene="tal"
                     /locus_tag="POA77_003516"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6366886.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            12647..14077
                     /locus_tag="POA77_003517"
     CDS             12647..14077
                     /locus_tag="POA77_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDI6366887.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14147..14923
                     /gene="yaaA"
                     /locus_tag="POA77_003518"
     CDS             14147..14923
                     /gene="yaaA"
                     /locus_tag="POA77_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDI6366888.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         3796 a         3722 c         3558 g         3927 t
ORIGIN      
        1 tttatccgat aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc
       61 catctctttt tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg
      121 tacgagctat caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt
      181 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
      241 tgataaacac tatcatcaga gtgattataa tgatttttat gatattggca gaaaaaatag
      301 cctttctgcc aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
      361 gctttggcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc
      421 caataactgg caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa
      481 taaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat
      541 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
      601 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      661 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      721 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      781 tggtggaagt attagtagtg gattagttgt ctggtccggt ggtattaata tcactaacca
      841 gttatccgat acatttgcaa ttctggatgc gcctggatta gaaggtgcac atattaatgg
      901 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      961 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
     1021 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     1081 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
     1141 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
     1201 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
     1261 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     1321 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
     1381 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
     1441 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
     1501 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1561 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1621 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1681 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1741 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1801 agggaataat actaattgta atagcatccg ctttaattat aatttaaata ctattttaca
     1861 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1921 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1981 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     2041 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     2101 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     2161 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     2221 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     2281 ggttatgtac aactaatcta tctcccccac cttaactttg gggggaggtg ccgattaacg
     2341 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     2401 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     2461 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     2521 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2581 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2641 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaatg gtttttctgg
     2701 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2761 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2821 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2881 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2941 accaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     3001 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     3061 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     3121 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     3181 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     3241 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     3301 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     3361 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     3421 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     3481 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3541 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3601 ggcagaatgg aggtcaagcc atccagcgtg acaccttgca gtgaaacgcc agcattagca
     3661 aatgcaaaca gcggcaaaat cagatacgcc acccacgggt gtaacacatg ctccagtcgc
     3721 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3781 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacactac gccaaccaga
     3841 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3901 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3961 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac
     4021 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     4081 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     4141 gccggaacaa tcatcccacc aatagcggcg ataactggaa atgcggcctg gcgtaagctg
     4201 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     4261 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     4321 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctattg
     4381 gccataatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     4441 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     4501 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4561 tttttacgat ccattgaaga atttgcgcca tcagcggtga gatgagtttt tgacggctgt
     4621 aagcgctcac gagtgaccct aagcccagat aatcatagct taaggtgagg atttctgaga
     4681 gattaattag gaaaagtaat aattaatttg ttgtcagtcg gcgatgtttg ttaggccaat
     4741 atttcaaaaa taatccagct tgtcgtcaat aattgcgatg aagagataaa ttaaagttaa
     4801 ataattaaat cattaaaaaa ttacgccata aaaatgcgag gcgtcttcag gattagaatc
     4861 gataaaacag aatgggttaa agagagcgat ataatagcgg cgggtgcttg aggctgtctg
     4921 tctcaggcat tagctgaacg gcagatagag aaaagccccg agtgatattt taccatcaac
     4981 ccgaggcccc ctatatgctg aacacatgta gagtgcctct tactgaccgt aaggtcaagg
     5041 agaagagagc aatgaagcag cataaggcga tgattgtcgc cctgatcgtc atctgtatca
     5101 ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg tgaggttcac atccgaactg
     5161 gccagacgga ggttgctgtt ttcacggctt acgaatccga gtaagagcaa cggcggggag
     5221 tgatccccgc cactttacag gtgctcgcat atcttcaacg caccctattt ttacccaggc
     5281 ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac
     5341 accatcaaag aagctctttg agcgcgggct gttgtgctca ccggttgggc caccgaagct
     5401 ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aggcctaccg gtgtttcgac
     5461 gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
     5521 gcgcatacgg aaaagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
     5581 atcaagggtc ggtacttcga tttcgccacc cagcgccgcc atagcgaagt tgatcgggac
     5641 ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
     5701 aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg
     5761 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
     5821 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
     5881 acagtgtgga caggtctgct gtacagcgaa gaatccctga cgcatctgca cctgaccaga
     5941 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
     6001 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
     6061 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
     6121 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
     6181 gccgccaaaa ccgccgccgc ccataccacc ttgctcaaac gcagcatgac catactgatc
     6241 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
     6301 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
     6361 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
     6421 ataatcttgc ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga
     6481 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
     6541 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
     6601 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
     6661 aaacctgtgc cagctcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
     6721 cagtttccag tgcagtcagt gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
     6781 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
     6841 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
     6901 ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatggtgatc ttctgctctt
     6961 taccgctgtt tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
     7021 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
     7081 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
     7141 cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt
     7201 ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc gatacccagt gacagcgggg
     7261 taacgtccag cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag
     7321 caccgattgc tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact
     7381 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt
     7441 cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
     7501 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
     7561 gtttcggacc ggtcgcatct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg
     7621 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
     7681 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccaggtag ttgatcagac
     7741 ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggtagccaga acttcgaagg
     7801 ttttttcgcc gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccaccaccca
     7861 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
     7921 ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt
     7981 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta
     8041 ccggttcacc caagtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaaa
     8101 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
     8161 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
     8221 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
     8281 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaataata gaaggcgtgg
     8341 tgcgatcgcc ttcggcgttc tccagcacgc gaggagtggt gccatccata atcgctacac
     8401 aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat
     8461 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
     8521 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
     8581 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
     8641 atagcaggac aagacgtgaa actgacagca attttattca tataaagaat gaatcgcagc
     8701 caagacattc atcaaaaaat tgtaataaaa agaaaacatt acgcacctga atcttctctt
     8761 tatcagcagt aaactagtgg gtatccatcc ccctacctct ccccactaag agaatcctta
     8821 tgaaatccgt ttttacgatt tccgccagcc tggcgattag tctgatgctg tgctgcacgg
     8881 cgcaggcaaa cgaccataaa atcctcggcg tcattgcgat gccacgtaac gaaaccaacg
     8941 atctggcgct gaaacttcct gtttgccgaa ttgttaaacg catacaactc tctgccgacc
     9001 atggcgattt acagttaagc ggtgcatcgg tttatttcaa agccgcccgt agcgccagtc
     9061 agagcctgaa tattccttca gaaataaaag aagggcaaac cactgactgg atcaacatta
     9121 acagcgataa cgacaataaa cgctgcgtct caaaaatcac cttttccggt catacggtga
     9181 actcatcgga tatggccacg ctgaaaatta tcggcgacga ctaacgccag aatatagaag
     9241 ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc tggattttct ccagcattgc
     9301 agtgaggaac agttggcaaa tttcgcccga ttgctcaccc ataatgaaaa aggcaaaact
     9361 cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg gcatcccgag
     9421 caacatcgcc gcaactggca gctgattgct ggagaattac agcattttgg tggcgatagt
     9481 atcgccaaca aactgcgcgg acacggtaaa ttgtatcggg ccattttgct cgatgtttca
     9541 aaacgattga agctgaaagc cgacaaagag atgtctacgt ttgagattga gcaacagtta
     9601 ctggaacaat ttctgcgtaa cacctggaag aatatggacg aggaacataa acaggagttt
     9661 ctgcacgcgg tcgatgcgag ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa
     9721 gacaaattat tggcaaaagg cgtgtcgcat ttgctttcca gccaactgac ccgcatttta
     9781 cgcacccacg cagcaatgag cgtacttggg catggtttgc tgcgcggcgc ggggctggga
     9841 ggccctgtag gtgcggcact aaatggggtt aaagcggtca gcggcagcgc ctatcgcgtg
     9901 acgattccag ccgtactgca aatcgcctgc ctgcgccgga tggttagcgc cactcaggtc
     9961 tgataccgta aaccggggaa aattttcatt ttcccctcaa aagatcgtag acactgcccc
    10021 actggctgat tattatgccg cgccctgaaa acactacagt tattcaggga aattatttca
    10081 ccattcattc gatgatgatt tttgaggaat tatgggcaac actaagttgg ctaatccggc
    10141 accgctgggc ctgatgggct tcggcatgac caccattctg cttaacctgc acaacgtggg
    10201 ttatttcgct ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca
    10261 aatttttgct ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac
    10321 ctcttacggt tctttctggc tgacgctggt tgcgattctg ctgatgccga aactgggtct
    10381 gaccgatgcg ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcgtatttac
    10441 gctgtttatg ttcttcggca cgctgaaagg cgcacgcgtt ctgcaattcg ttttctttag
    10501 cctgaccgtg ctgtttgccc tgctggcgat cggtaacatt gccggtaacg ccgcaatcat
    10561 ccactttgcc ggctggattg ggctgatctg cggtgccagc gcaatctatc tggcgatggg
    10621 tgaagtactg aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt
    10681 gttttgagat ccccccgctc ggggggattt ttttattcgc taacgtcgcg tcttgcactc
    10741 ttcggtctga atgctgcaac cacttccggt gccgtttcaa cgtatggccc ttccagcaac
    10801 tgaatgcagt acggtacgct ggcaaaaata ccgtgtacca caacgttacc cgcagcgtcc
    10861 ttcacacctt ccagcgtctc tttaatagac ttcggctgac cgggtaagtt aaggatcagc
    10921 gcctgtttgc gaatcacacc aacctgacgc gaaaggatcg cagttggtac aaaatgcagg
    10981 cttatctggc gcatctgttc accaaagcca ggcatctcgc ggtccgctac tgccagcgtc
    11041 gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag caccagatgg
    11101 caactcattt catccactag ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg
    11161 attaagcggg tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagc
    11221 gcagggatgc ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag
    11281 ccaatacgta aagtattcat caagattttt ccgtttaaac agtctcgtta aacagaatga
    11341 tacactgcga agggagtgac agacaggctt cagaagaggt agcgtgaccg acttcccggt
    11401 cacgctaaga atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaata
    11461 gcaaacttac ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc
    11521 cacaggaact cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac
    11581 agtttacgtt cgatagcccc ttcgctctca gccagctctt tcagcagtgc cggtgcgatg
    11641 gtcagacggt cgcagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc
    11701 ataaccacgg tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc
    11761 acgcccggat cttctgccgg agcgtactct ttcttatcag tattcgcttt gtaccagtca
    11821 agaatacggc caacaaacgg cgagatcagg aacacgcccg cttccgcaca agcacgagcc
    11881 tgagcgaagg agaacagcag ggtcaggtta cagttgatgc cttctttttc cagctgttct
    11941 gcagcacgga taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata
    12001 ccagcatcgt tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttcagtgtca
    12061 taggaaagac gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc
    12121 agaccaatat ttactgccag tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg
    12181 ctctgctgtt tcgcccaggc gacagcatca tcaatcaact tacggtattc cggaatctgc
    12241 gctgcgttaa gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt
    12301 gccgcgatgt ccccagtgtc ggccactacg gtggtgtact gacgaaggga ggtcaatttg
    12361 tccgtcatga tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct
    12421 gatgatatca cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga
    12481 ctgtttcgga tttctgacac tgcgttgata tcgcccgcca tttttataca aaacctcatg
    12541 tatgctacgc agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa
    12601 cgtttacaca ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt
    12661 ctcctttatt aacagcgtcc tttggggatc ggtaatgatt tacctgctct tcggcgcagg
    12721 ttgttggttc acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa
    12781 aagtcttaaa aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg
    12841 taccagtctt gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggcaattac
    12901 cgccggtgga cctggagccg tcttttggat gtgggttgcc gcgtttatcg gcatggcgac
    12961 ctcgtttgcc gaatgttctc tggcacaact ttataaagaa cgtgacgtca atggacagtt
    13021 tcgtggcgga ccggcatggt atatggcgcg cgggctgggg atgcgttgga tgggcgttct
    13081 gttcgccgtc tttttgctca tcgcctatgg cataattttc agcggagttc aggcgaacgc
    13141 cgttgcccgc gccctaagtt tttcttttga ttttcctccg ctggtgacag gcattattct
    13201 cgctgtcttt gctctgctgg caatcactcg cggtcttcat ggcgtcgccc ggctcatgca
    13261 ggggtttgtc ccgttgatgg cgataatctg ggtgctgacc agcatggtga tttgcgtaat
    13321 aaatatcggg caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca
    13381 ggaagcggca ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca
    13441 gcgcagtatg ttttccaatg aggcgggaat gggttcgacg ccaaacgcgg cagcggcagc
    13501 ggcgtcctgg cctccgcatc cggcagcgca agggattgtc cagatgattg gcatttttat
    13561 cgacaccctg gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacgacac
    13621 aacctacatg ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg
    13681 ttcctggggt gctgagtttg ttaccctcgt ggttattctg tttgccttca gctccatcgt
    13741 tgccaactac atttatgccg aaaacaatct cttcttttta cgcctgaaca accctaaagc
    13801 gatctggtgt ttgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagcct
    13861 tccgctgatg tggcaactgg cagatatcat aatggcctgc atggctatta ccaatttgac
    13921 cgccatttta ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg
    13981 taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg gtcgccagct
    14041 ttctccggac gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt
    14101 ctcttttttt gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat
    14161 ttcacctgcg aaaacgcttg attaccaaag cccgttgacc accacgcgct atacgctgcc
    14221 ggagctgtta gacaattccc agcagttgat ccatgaagcg cggaaactga cgcctccgca
    14281 gattagcacg ctgatgcgca tcagcgacaa actggcgggt atcaacgccg ctcgctttca
    14341 tgactggcag ccagatttca cgccggagaa tgcccgccag gcgattctgg cgtttaaagg
    14401 tgatgtctac accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca
    14461 acagcatttg cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg atttaatgca
    14521 gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca
    14581 attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa
    14641 tgtggtgatt aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa
    14701 tgccgagatt atcaagccgg tgttcctcga tgagaagaac ggcaaattta agataatcag
    14761 cttctacgct aagaaagcac gcggtctgat gagtcgtttt attattgaaa atcggctgac
    14821 caaaccagag caactgactg gttttaatag cgaaggttac ttctttgatg aagcttcctc
    14881 cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg
    14941 gatgcggcgt gaacgcctta tccggcctac aaaagcatgc agattcaata tattgcagaa
    15001 ttc
//
LOCUS       DAMZII010000111        14967 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZII010000111 DAMZII010000000
VERSION     DAMZII010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.111"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            117..359
                     /locus_tag="POA77_003519"
     CDS             117..359
                     /locus_tag="POA77_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366889.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            473..1111
                     /locus_tag="POA77_003520"
     CDS             473..1111
                     /locus_tag="POA77_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDI6366890.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1108..1845
                     /locus_tag="POA77_003521"
     CDS             1108..1845
                     /locus_tag="POA77_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDI6366891.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1845..3941
                     /locus_tag="POA77_003522"
     CDS             1845..3941
                     /locus_tag="POA77_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDI6366892.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRLSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3988..4266)
                     /locus_tag="POA77_003523"
     CDS             complement(3988..4266)
                     /locus_tag="POA77_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366893.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4481..4891
                     /gene="psiE"
                     /locus_tag="POA77_003524"
     CDS             4481..4891
                     /gene="psiE"
                     /locus_tag="POA77_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDI6366894.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4935..6410)
                     /gene="xylE"
                     /locus_tag="POA77_003525"
     CDS             complement(4935..6410)
                     /gene="xylE"
                     /locus_tag="POA77_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDI6366895.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6782..7672)
                     /gene="malG"
                     /locus_tag="POA77_003526"
     CDS             complement(6782..7672)
                     /gene="malG"
                     /locus_tag="POA77_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDI6366896.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7687..9231)
                     /gene="malF"
                     /locus_tag="POA77_003527"
     CDS             complement(7687..9231)
                     /gene="malF"
                     /locus_tag="POA77_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDI6366897.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9385..10575)
                     /gene="malE"
                     /locus_tag="POA77_003528"
     CDS             complement(9385..10575)
                     /gene="malE"
                     /locus_tag="POA77_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDI6366898.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10940..12055
                     /gene="malK"
                     /locus_tag="POA77_003529"
     CDS             10940..12055
                     /gene="malK"
                     /locus_tag="POA77_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDI6366899.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12127..13467
                     /gene="lamB"
                     /locus_tag="POA77_003530"
     CDS             12127..13467
                     /gene="lamB"
                     /locus_tag="POA77_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDI6366900.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13710..14630
                     /gene="malM"
                     /locus_tag="POA77_003531"
     CDS             13710..14630
                     /gene="malM"
                     /locus_tag="POA77_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDI6366901.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3713 a         3843 c         3929 g         3482 t
ORIGIN      
        1 taaaacccca tctctaattc cccgcgccgt aattcgcatg ctttagttgt gtatactcga
       61 tcccgcccga aatgtttttg ggtaaatctc cattcattca atgaagggaa attgttatga
      121 aaaaagttct gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg
      181 cacctgtaca ggtgggcgaa gcggcagggt cggcagcaac gtcggtttcg gcggggagtt
      241 cctccgcgac cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg
      301 gtggcggtga tggttctaat accgggacca ccacaaccac gaccaccagt acccagtaat
      361 aaagtatgta tccccaaaat aatttgagtc attgcatctg tggcttgaag tatgaaggga
      421 tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag tcgtgaagcg
      481 acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct
      541 gggcaattca ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga
      601 tattcaaaat atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt
      661 tgtggtgctg gccttcgctg aagacggaca acaaaaatgg gtcactcagg atcaagccac
      721 tcttgtcata caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga
      781 agtgaataac cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac
      841 atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc
      901 agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa ccccggttcg
      961 cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct actggatcga
     1021 cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa
     1081 aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc attgattttg
     1141 agtgtggggg cgtcatcggt cttcgcggcg ggaaccgtca aggtattcag caatggcagc
     1201 agtgaggcca aaacgctgac gggcgcagag tatttaatcg atctggtagg ccagccgcgg
     1261 ctggcaaaca gttggtggcc tggtgcggtg attagcgaag agctggcaac ggcggcagca
     1321 ttgcggcagc agcaggcgtt gctgacacgg ctggcagaac tggctgcaga ttccagcgcc
     1381 gacgatgccg ccgcgattaa cgccttacgc cagcaaattc aggcgttgaa agtgacgggc
     1441 aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg ccgaacgcgg taacccaccg
     1501 ttgcagggca actacacgct gtgggtcggg ccaccaccgt ccacggtcac gctgttcggg
     1561 cttatcagcc gtcctggcaa tcagccattc actccaggtc gcgacgtggc gagctatctc
     1621 tccgggcaga acctgcttag cggtgcggat cgcagctacg cgtgggtggt ttacccggac
     1681 ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga gccgatgccc
     1741 ggcagcatta tttatgttgg cctcgcggac tccgtctgga gtgagacgcc tgatgccctt
     1801 aacgccgaca ttcttcagac tctgacgcag cggatacctc aataatgaaa aaaagacatc
     1861 tgcttagctt actggcgctg ggcattagca cagcttgcta cggcgaaaca tatcctgcgc
     1921 ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt attacaaact cccaccgcgc
     1981 gtatggcacg ggaaggggag ttgagtctga actatcgcga taacgatcag taccgttatt
     2041 actcagcttc agtgcaactc ttcccgtggc tggaaacaac gctgcgctac accgacgtgc
     2101 gcacccggca gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat aaagataaag
     2161 ccttcgatct caaactgcgt ttgtgggaag agagttactg gctgccgcaa gtggcggttg
     2221 gtgcgcggga tatcggcggt acggggctgt ttgatgcgga atatcttgtt gccagcaaag
     2281 cctgggggcc gttcgatttt acgctcggcc tgggctgggg atatctgggc accagcggta
     2341 atgtaaaaaa tccgctctgt tcagccagtg ataaatattg ctatcgcgat aacagctaca
     2401 aacaggcggg atctatcgac ggtagccaga tgttccacgg tcctgcctca ctgtttggcg
     2461 gcgtggaata ccagacgccc tggcaaccgc tgcgcctgaa actggagtat gaaggcaata
     2521 attatcagca ggattttgcc gggaagctgg agcaaaaaag taagtttaac gtcggtgcga
     2581 tttatcgcgt taccgattgg gccgacgtta accttagcta tgaacgtggc aacaccttta
     2641 tgtttggcgt cacgctgcgc accaacttta acgatctgcg cctgtcttac aacgataacg
     2701 cccgcccgca atatcaaccg cagccgcagg atgccatttt gcagcattcg gtggtggcga
     2761 atcagttaac gctgttgaaa tacaatgccg gacttgccga tccgcagatc caggcgaaag
     2821 gcgatacgct gtatgtcacc ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca
     2881 tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg atccgcatta
     2941 cggaaaatcg tcttaacatg ccgcaggtga cgacggaaac cgatgtcgcc agcctgaaaa
     3001 atcatctcgc cggagagccg ttgggccacg aaacgaagct ggcgcaaaaa cgcgtcgagc
     3061 cagtggttcc gcagtccacc gagcagggct ggtatatcga caaatcacgc tttgatttcc
     3121 acatcgatcc ggtgctgaac cagtcggtcg gtggcccgga aaacttttac atgtatcagc
     3181 tgggcgtgat gggaacggca gatttgtggc tgacggacca tctgctgacc accggcagcc
     3241 tgtttgcaaa tcttgccaac aactacgaca agtttaacta caccaatccg ccgcaggact
     3301 cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat gtctatgtga
     3361 ataacctgca agccaactac ttccagcatc tgggcaacgg cttctacggt caggtctatg
     3421 gaggttatct cgaaaccatg tttggcggtg cgggggccga agtgttgtat cgtccgctgg
     3481 acagtaactg ggcgtttggt ctggatgcca actacgttaa acagcgcgac tggcgtagtg
     3541 caaaagatat gatgaaattc accgactaca gcgtgaaaac cggacatctg accgcctact
     3601 ggacgccatc tttcgcccag gacgtgttgg tgaaagccag cgtcgggcag tatctggcag
     3661 gggataaagg cggcacgctg gagatcgcca aacgctttga tagcggcgtg gtggttggcg
     3721 gctatgccac gatcaccaac gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag
     3781 gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg cccgacacgc agccgtgcgg
     3841 cgattggctg gacgccgctg acgcgtgacg gtggtcagca acttgggcgt aagttccagt
     3901 tgtatgatat gaccagcgac cgtagcgtaa atttccgcta agtcatggga aaggtgccag
     3961 ttttcgcact cactggcacc ctcattctta atgacctgtt gagccatcag gaacgcagtt
     4021 cacccccggt ttacgatctg gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga
     4081 tatcggttgc aggtctggtt gcctgagcga ctgggccatc attgggctgg agacattgct
     4141 ttgcggtagg tcggctttat cagcagtacc gagcggacca gcataagcag gaagaacaga
     4201 gactgataac atcaaagcag caaaataagg cttcattttt accaccttta tcaggttacg
     4261 tttcatttgt tccagaggaa cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca
     4321 cctgaatggg tgattttttg gggctgtgtg ttttgccaga tgcgacgttg atgggagatg
     4381 cccacttaat caagctgaac aaaacataca caaaaaatat agatctccgt cacatttttg
     4441 cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc
     4501 gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc tgttgtgcct
     4561 cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggcag atgtgctgtt
     4621 cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt actttctcta
     4681 tttcgaattt atcgcgctga ttgtgaaata ctttcagtcc ggttttcact tcccgctacg
     4741 ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc
     4801 gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca ctctgtggtt
     4861 gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt
     4921 gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag
     4981 agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat
     5041 agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg
     5101 gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc
     5161 agccactggg ccgccaccgc gattgccagc gctttaccac gaatggcatt cgggaagatt
     5221 tccgacagca gaacccagca taccggaccc caggacatgg caaaggcggc aacatagaac
     5281 agcatcgaca gtagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta
     5341 aacataccga ttgccattcc gagcgcgccg ataatttgca gtggcttacg accaaattta
     5401 tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc
     5461 tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac
     5521 aacaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc gattacaatc
     5581 acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt
     5641 ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatactt
     5701 tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac
     5761 agcagcatta agaacagcag tgcagggata cattccgagg caaacatata acgccagccg
     5821 tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac gcagtaaact
     5881 aaaagttgcc cgaaaataat cgcaaactgg ttaaatgaaa ccagttttcc gcgaatatga
     5941 gctggagcca gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata
     6001 ccgccaataa tacgataaat aacaaattcc gggacataac ctgccagata aatagggact
     6061 gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa
     6121 ataaaaaaca ggacagcagc aatcttaagt gaatcacgac ggccgaagcg gttactgcaa
     6181 taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggctac gcaaaatcct
     6241 aacagggagt tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtattgagt
     6301 gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta
     6361 gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc
     6421 cttagatcat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc
     6481 aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa
     6541 ttatccaaaa ttgataaaaa caatactatt gccgtgattc agagcacgaa agagaattat
     6601 cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatgggaa tattaaccgg
     6661 cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc cgaataatag
     6721 tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc
     6781 tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcaaga
     6841 agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt
     6901 agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagcta tttacgtcac
     6961 gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca
     7021 ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg
     7081 tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt
     7141 tgatggtcca gacatgcagc gcaatcccac ccagatacgc gaaaattacg ccgccatgag
     7201 tattcagacc aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga
     7261 ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt
     7321 tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc
     7381 caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg
     7441 gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc agtttccagt
     7501 gatcccagga gatttgctcc gggatcaggc taccggtcgc gaagtttccc tgacgcagcg
     7561 agatagcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca
     7621 ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta
     7681 tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacccac
     7741 cagcaggaag atcagcgtgg caattgctgc cgccagaccg aagtcctgac ccccgccgcc
     7801 ttcaaaagcg atacggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt
     7861 cgtgccaaga cgatccgggc cgccgttggt taacagctga atcagcacga agttgttaaa
     7921 gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt
     7981 aatcttaaag aagttctgga acggacctgc gccatccatt gctgaggctt catacaaatc
     8041 gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca
     8101 ggtattgacg ataatcagca tcgtgcgggc ggtggtcgga tcgctgaacc aggcgggctt
     8161 cacgccaaac agcgcgctca acatcatgtt gatttcaccg aagctctggt taaacaatcc
     8221 tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca gcaggacgcg
     8281 atagaccgct ttgccgcgca acgcttccca ctgcaccaga catgccagaa ccatgccgac
     8341 cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag
     8401 gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc agccggtggt
     8461 cacggtgtaa ccggggctta gcttttcatc accccagttg ccgtcggcgg taatggactg
     8521 gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat tcgtcaacgt
     8581 gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat
     8641 catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg
     8701 ggtaatcacg cgcagattcg cgcgttcgcc ttcgggctga gcggtcgttt ctttcagttg
     8761 cagtttttgc tcgccgccaa atttaaacgc gtcggagagg taatttttgc cggtttcgcc
     8821 gtcgctgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt tataggtttt
     8881 gcctgcttgc caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt
     8941 agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga agaggacgaa
     9001 taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc
     9061 gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc
     9121 ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac ctagcactga
     9181 ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt
     9241 cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac
     9301 actgatgcgt cttatcaggc ctacatgcgt ttcagttttg taggccggac aaggcgttca
     9361 cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc
     9421 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca
     9481 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt
     9541 ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ccactgcacc
     9601 cagcggtttg tctttattga ccgcttccag accttcgtca gtcagcagat agttttcgag
     9661 gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc tcagcacgcc
     9721 aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
     9781 tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc
     9841 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
     9901 aataaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
     9961 tttaatatcg tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag
    10021 cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt
    10081 cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg gcagcagatc
    10141 tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca gcttgccgtt
    10201 gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt
    10261 gatttcagcc aacaggccag attgagcgta gccaccaaaa cggtcgtgcg cccagaagat
    10321 aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg
    10381 atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag
    10441 accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc
    10501 gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc
    10561 tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac
    10621 ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg
    10681 ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc
    10741 gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag
    10801 aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt
    10861 aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt
    10921 gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg
    10981 cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt
    11041 tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac
    11101 gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacactc cgccagcaga
    11161 acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga
    11221 aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt
    11281 taaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct
    11341 ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg agccaagcgt
    11401 atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat
    11461 cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca
    11521 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca
    11581 ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat
    11641 cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt
    11701 gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg aaagccgtga
    11761 tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
    11821 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
    11881 ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
    11941 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
    12001 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
    12061 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
    12121 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
    12181 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
    12241 acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
    12301 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
    12361 ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca acagaatgac
    12421 tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa
    12481 tggctgccag gttccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat
    12541 atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat
    12601 gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagctgg tggttcttcc
    12661 tcttttgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgat
    12721 gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac
    12781 ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggctgg
    12841 ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag
    12901 tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg
    12961 tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc
    13021 gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag
    13081 gatatcaact gggataacga caacggcacc aagtggtgga ccgttggtat tcgcccgatg
    13141 tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc
    13201 cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct
    13261 ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat
    13321 gagaaatggg gttatgacta caacggcgat agcaaggtta acccgaacta cggcaaagcc
    13381 gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc
    13441 ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag gcgtttacgc
    13501 cgcattcggc aaccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct
    13561 gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca ggttgtcatt
    13621 atctgaaagg ggcgaaagcc cctctgatta tcgggtttag cgcgctattg cctggctacc
    13681 gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct
    13741 gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat gttaactacg
    13801 taccgcaaaa caccagcgac gcgccagcca ttccatctgc tgcgctgcaa caactcacct
    13861 ggacaccggt cgatcaatct aaaacccaga ccacccaact ggcgaccggc ggccaacaac
    13921 tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca aacattggcg
    13981 aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgttttt gcgccgaacg
    14041 tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt tatttcacct
    14101 accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc ctgacaccgg
    14161 cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat ctccagcaga
    14221 cgacccaact gctcgacccg gctaaagcct atgccaaggg cgtcggtaac tcgatcccgg
    14281 atatccccga tccggttgct cgtcatacca ccgatggctt actgaaactg aaagtgaaaa
    14341 cgaactccag ctccagcgtg ttggtaggac ctttatttgg ttcttccgct ccagctccgg
    14401 ttacggtagg taacacggcg gcaccagctg tggctgcacc cgctccggca ccggtgaaga
    14461 aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg
    14521 tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctgggat
    14581 cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca
    14641 cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttccttc
    14701 ctgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa tatccattat
    14761 ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca ttcaggctac
    14821 ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat atcctaaatt
    14881 ctggcggcaa taactctttg atgaaacatg atgtgatgca aggaaataat atagtagatc
    14941 ttgatttact acgtaatttc aatgggg
//
LOCUS       DAMZII010000112        14814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZII010000112 DAMZII010000000
VERSION     DAMZII010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.112"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            188..1282
                     /gene="ddlA"
                     /locus_tag="POA77_003532"
     CDS             188..1282
                     /gene="ddlA"
                     /locus_tag="POA77_003532"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6366902.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1306..1518)
                     /locus_tag="POA77_003533"
     CDS             complement(1306..1518)
                     /locus_tag="POA77_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDI6366903.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1778..2086
                     /locus_tag="POA77_003534"
     CDS             1778..2086
                     /locus_tag="POA77_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDI6366904.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2145..3239)
                     /gene="yaiW"
                     /locus_tag="POA77_003535"
     CDS             complement(2145..3239)
                     /gene="yaiW"
                     /locus_tag="POA77_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDI6366905.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKDPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3252..4472)
                     /gene="sbmA"
                     /locus_tag="POA77_003536"
     CDS             complement(3252..4472)
                     /gene="sbmA"
                     /locus_tag="POA77_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDI6366906.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4824..5981
                     /gene="ampH"
                     /locus_tag="POA77_003537"
     CDS             4824..5981
                     /gene="ampH"
                     /locus_tag="POA77_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDI6366907.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVADRGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5982..6605)
                     /gene="iprA"
                     /locus_tag="POA77_003538"
     CDS             complement(5982..6605)
                     /gene="iprA"
                     /locus_tag="POA77_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDI6366908.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6693..9599)
                     /locus_tag="POA77_003539"
     CDS             complement(6693..9599)
                     /locus_tag="POA77_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6366909.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWTYIDNPVTALD
                     WDHMDKAGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYADSTAN
                     QLDISNSVIHGSITSMLPGGYYDRFDADGNNLGGYDFYTDAVVDTHWRDGDVFTLNIA
                     NTTIDDDYEALYFTDSYKDGDVTKHTNETFDTSEGVAVNLDVESNINISNNSRVAGIA
                     LSQGNTYNETYTTESHTWDNNISVKDSTVTSGSNYILDSNTYGKTGHFGNSDEPSDYA
                     GPGDVAMSFTASGSDYAMKNNVFLSNSTLMGDVAFTSTWNSNFDPNGHDSNGDGVKDT
                     NGGWTDDSLNVDELNLTLDNGSKWVGQAIYNVAETSAMYDVATNSLTPDATYENNDWK
                     RVVDDKVFQSGVFNVALNNGSEWDTTGRSIVDTLTVNNGSQVNVSESKLTSDTIDLTN
                     GSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGADYNLYANTITVTNGGVLDVNVDQ
                     FDTEAFRTDKLELTSGNIADHNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYGY
                     GIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKD
                     KEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSAN
                     TNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVD
                     TKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYS
                     HFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDD
                     YQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNG
                     DSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            10123..11097
                     /gene="hemB"
                     /locus_tag="POA77_003540"
     CDS             10123..11097
                     /gene="hemB"
                     /locus_tag="POA77_003540"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI6366910.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11203..12054)
                     /gene="tauD"
                     /locus_tag="POA77_003541"
     CDS             complement(11203..12054)
                     /gene="tauD"
                     /locus_tag="POA77_003541"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDI6366911.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12051..12878)
                     /gene="tauC"
                     /locus_tag="POA77_003542"
     CDS             complement(12051..12878)
                     /gene="tauC"
                     /locus_tag="POA77_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDI6366912.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPLQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(12875..13642)
                     /gene="tauB"
                     /locus_tag="POA77_003543"
     CDS             complement(12875..13642)
                     /gene="tauB"
                     /locus_tag="POA77_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDI6366913.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13655..14617)
                     /gene="tauA"
                     /locus_tag="POA77_003544"
     CDS             complement(13655..14617)
                     /gene="tauA"
                     /locus_tag="POA77_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6366914.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3763 a         3853 c         3543 g         3655 t
ORIGIN      
        1 gaataaatta gcttcccgtt catcattttt gtgatcttga gcacatcttt gagataacgc
       61 actttcttca ccagtacgtt gcacgctttg tcagcagaag attgcaacaa tctttcagaa
      121 ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt
      181 ttttaagatg gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga
      241 agtgtctctg caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt
      301 tgtgctgctg ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct
      361 gctaaatgca gacgatcctg cccatattgc gttacgccct tcggcgacca gccttgcgca
      421 ggtgccaggt aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttgccgac
      481 ggtggatgtc attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg
      541 aatgctgcgg gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg
      601 tatggataaa gatgttacca aacgtctgct gcgcgatgcc gggctgaaca ttgcgccatt
      661 tattaccctg acgcgcgcca atcgtcacaa cattagtttt gccgaagtgg agtctaaact
      721 ggggttacca ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt
      781 aaccagtgaa gaacagtacg caattgccgt cgatctggcg ttcgaattcg atcataaagt
      841 gatcgttgag caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa
      901 cccgcaagcc agcacctgtg gcgagatcgt actcaccagc gacttctatg cctacgacac
      961 caagtacatt gacgaagatg gcgcgaaagt ggtagttccg gcagccattg caccagagat
     1021 caacgataag atccgggcga ttgccgttca ggcttatcag acgttgggat gcgcaggcat
     1081 ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac
     1141 actgcctggc ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctcgg
     1201 ttacaccgat ctgatcacgc gtttgattga gctggcgctg gagcgtcacg ctgcggataa
     1261 cgcattgaaa accacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc
     1321 agtttcttca tcgcgacgac gaataatcag ccccgccagc cagaaactaa tcacccacgt
     1381 caccagaccg acggcatagg tctgccaacc ttttgcttca aatcccagta accccaccac
     1441 gccattaaga atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat
     1501 ttttacaggc agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt
     1561 aacaaccgtc tgtggtgtag acaacatttt cgtaattgtt aataaattta acttaaggtt
     1621 gccattttgt gactcacaac gaagattcac gcttcgggaa tattcccaac atcaatttac
     1681 catgattctg atattgacaa tctaaatgac ctgtcagact tgccataagc cgctggtatc
     1741 acaccagaag agaagcagat gacttccgga ggtttttatg gctgatttca ccctgtcaaa
     1801 atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc
     1861 attgtttgtg ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc
     1921 gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat
     1981 tggtttaggt gtcggcacca ttttcgggct gatcccgttt ttagtaggct gcctcatttt
     2041 tgcagtggtg gcgctatggc tgcactggcg acatcgccgc cagtaagatt tacgttgcca
     2101 gatacggcct aaaacgcctt atctggcatg acattcctgt ttgatcactg tttcatacaa
     2161 cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt
     2221 ttcgggcttt ccagttgaat gccaggcaac atttcgcggg gtagtgattt acccgttttc
     2281 gcttcggcaa gttgataaac tttcttgtac agcgccgtct cctcaaaaga gaagctgtca
     2341 cctttctcta actgacggcg aatttcgctg tcgctcatcc ccagttttgc cgccagtttg
     2401 cgcgttgcca gttcggtttt cccgggatct ttactgtcgt aacgaattaa atcaccgtcc
     2461 agtgccagct tcacgccgct ggccttactg accgcgttct gaaacgcggc attacggctg
     2521 gcgtaccagc cagcgttaaa atcagcaaag cggtatatcg gtgcgctata gctggcggga
     2581 taattcagta aatggtaagt accaaaccac aacccgccgc ggcgactgaa gacttcctga
     2641 cgaactgtac cgtccatttt ccacggatac cctttggtat gctgttcggc aaaagcaatg
     2701 ctgacctgca tcggcccacc ggtgcgcaca ggattaagcg aaccaaacag cgtctgcccc
     2761 attggtacca tgctgatcag atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc
     2821 gaatctaacc gttcgctata acttttgccg tttggcgatt tgatttttag cgcggtatga
     2881 accagaaaag caggaatgtg catccgttca gcacgacggt caatctcttg ccaggcgatc
     2941 ttgcttaagc ccggaacggc cggatctgcc tgataatttg actcctgctg cgccaccgcc
     3001 agcaccgagc agacgttttc cagcgtcggt gccagcccct gactttcaaa ggtggtggcg
     3061 agatctttcg cccaggcatc gcggtctttc acgctcgcag gcatcttctg ccgcacgacg
     3121 ctcgctacat ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg actactacac
     3181 cccgctaaaa ccagaaccgc caacaatgac agcgaagata gatgattgac gcgcgacatt
     3241 acttctcctt tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc atccagctca
     3301 tgttcaaagc tgcgcagacg tttgtagata gacatcaact caaccagtgt ggtccatgag
     3361 ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc
     3421 aggccgagcg taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg
     3481 ttatcgacct gcaaatagag aatgcgggcg atgttgaaat acatatagtg aaaatagagg
     3541 cgaaaatagt ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc
     3601 gtggcatcat cttcaccata aaccagctct ttacggtagg cagcctctac acgctggttt
     3661 ttaaactcca gccccggcag tttgatccct accactgcca gcaatccggt ccccatcagc
     3721 gaccagacga tagcggcaat caccagacca tacggaatgt gcccgataat cggcagctcc
     3781 ggcacatgcg cggagagcgt taccagtacc ggcaggaagg cgattaacgt catgatggcg
     3841 ttgataaaac tgacgcccat attctccagc gttgaagcaa aacgcatggt gtcttcctgc
     3901 acacgctgtg cggcaccttc gatatgacgc agttgttgcc agttcgccat gtaatattcg
     3961 ttcatcgccg tacgccagcg gaacacgtag tgactgacaa agaagttatt cagcacactg
     4021 atcactaccg cgatcagcgc aatccccaga aagacgccca cttcgcggta aaattgttcg
     4081 atggtgactt tatgcggcga acttagcgcg gtttgaatca gatcatagaa cggcgcatac
     4141 caggcgttga cggcaacccc gacttccacc aaaaaccagg tgacgaagat aatcagcgca
     4201 gtaccgagta ttgaccagta ttgccaacga tgcgggctgt agataaacca gaacaatgca
     4261 aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa
     4321 cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga tacgcgccac ccagtcaccc
     4381 ccaccggctt gccagaagat aacggcaatc aatgcccaaa caaaggccga gagaaaaaac
     4441 gttcccggct ttgggaaaaa agacttaaac atatgcactc ctgctaactt cttatcgttt
     4501 taacaactgc tgtgtattaa ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc
     4561 cattgtgacc catagcaagg cgcttagttc gccagcctga ccataagaat tgtttcgacg
     4621 ggtttcacaa accaaagatt gtttacataa gcggattaac acgcgcagga tcacaaatgc
     4681 aggcattggt gagcaggaaa aaaatcgtgt ttacgcgccg ttacgttttt aacactatta
     4741 ttgcactaat tcgccctttg caatccatca atgagtagta taaatacgct cagttacctt
     4801 cattcaatct atggacacca ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg
     4861 cggcgtcatt gacctccgtc catgcggcgc agccgatcac tgaaccggag tttgcctctg
     4921 atattgtcga tcgttatgcc gatcatattt tttacggcag cggtgccacg gggatggcgc
     4981 tggttgttat cgacggtaac cagcgcgtct ttcgtagtta tggcgaaacg cgacctggta
     5041 ataacgttcg cccgcagctg gattcggtcg tgcgtattgc ttccctcacc aaactaatga
     5101 ccagtgaaat gctggtgaaa ttgctcgacc aggggaccgt aaagttaaac gatccgctaa
     5161 gtaaatatgc tccgccaggc gcacgcgtgc cgacctacaa tgggacgccc attaccctag
     5221 tgaatctggc aacccatacc agcgccctgc cccgtgaaca gcccggtggc gcggcacatc
     5281 gtccggtgtt tgtctggcca acgcgcgaac aacgctggaa atacctttct acggcgaagc
     5341 taaaagccgc gccaggcagt caggcggcgt actctaacct tgcgttcgac ctgctggcag
     5401 atgcactggc gaatgcgtcc ggcaagcctt atacccagtt gtttgaagag cagattaccc
     5461 gtccattggg gatgaaagac accacctaca ccccctcacc ggatcagtgc cgccgtttaa
     5521 tggttgctga tcgcggtgcc agtccgtgca ataacacgct ggcggcaatt ggcagcggcg
     5581 gcgtctattc cacgcctggc gatatgatgc gctggatgca gcagtatttg tcatctgatt
     5641 tttatcagcg cagtaaccag gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt
     5701 ttacgaaagt gattggcatg gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg
     5761 tatatatggc cccgaaagag ggtcgtccgg ggattattca gaagacagga ggtggtggcg
     5821 gtttcattac ctatatggcg atgatcccgc agaagaatat tggcgcgttt gtggtggtca
     5881 ctcgttcgcc gttgacgcgc tttaaaaata tgagcgatgg catcaatgat ctggtgaccg
     5941 agctaagcgg gaataaaccg ttggttatcc ccgcatcctg attaatactc tgaaggcaaa
     6001 cggttgatag cgaccagttt gcctttattc atttcgatat agccgccttt acgcaaagca
     6061 gcgagaactt ctgcgacgac agaacgcgat atgcgtgtac gttgatggat ataattcatc
     6121 acaccaatac gtgagcgcaa ttcttcattc cagtcaatca ttgataataa tgttgcgcgg
     6181 atttgttcgt aggaattatg cccaatgagc tgcacgtcgc gtaattccag aattctattt
     6241 tgccaggcta accagtaaaa agcgtctcgc cagagttgat tttgttcaat aagcgtaatg
     6301 gtttgtttgg ctggtagatg atatcccgta caatttcctt ctgatattaa tttgtatggt
     6361 atatcgtttt tcattaaacc atcagccagc cccataatat aaggagcctg ggtaataccg
     6421 ataagtacgt tttcttctct acgcagagag ataacgccct ctaaaataac gaaagtatct
     6481 tcgctattga catcactgga aaatataact tgcttttcat tattaaactc gaagcgcgta
     6541 ccgtatctgg acaaacattt atcgagctta ccaaattcct gaagaggttt aactacagat
     6601 aacatttgcg cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat tatttagatt
     6661 aaattaccag aatttcttcg gagtgaagaa tattaccagg tatatttaac acccacgttc
     6721 gcggaccagt cttgatctac gtcaccacca ccgaggtagt tagcatcggt ataggcgctg
     6781 aagttcttgg tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga cccttcagta
     6841 ccgttatcga tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa
     6901 gccagtttga agtacggagt cagagcctgg tcttcgctgt aggtgaaggt ataacctgca
     6961 tctacaccca gttcataacg catgctgtcg taagactgac cgtcaacttt catgtcgttg
     7021 ctcagctggt agtcatcacc agactggaac agaccagaaa tgctgccgta aggagtcacg
     7081 taaccagcat cacccagttt gaagtcgtaa ccggctttca aaccgaagcc ccaagcgtcg
     7141 gagttggtgc taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg
     7201 aagtgagagt aactcaagct accatcaaca aagacgttgt tcgcgaagtg agcagaagag
     7261 tagatgtagg cagtctggct gtcttgatcc acctgaccag aacggtcatt catgtcacct
     7321 ttagcgaagc ctgcagccgc accgacgatc cacttagcgt tgttaccgtc aattttggta
     7381 tcaacaccga ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc gttgtcgccg
     7441 ttgaagttac caccgaagta gcttacccat gcgccgccgt tatcagccag gccatgacga
     7501 gagttggtca gacgagtacc aacggtgtct tgttccaggt tccagatatt ggtgttcgca
     7561 gacgggatgc tcagcgccat gttagcgtag tcggtcagct ccatctgttg cagaacaacg
     7621 gtgttaccgc gctgttcagc ctgataggtg tatgcaccca ggtcagcttt gttagcagca
     7681 gagaaggtcg cgttggtgtt gacgtcgttt acgtagataa tttctttatc tttgtaatca
     7741 gcgatagcgc cagcgccagt tgcgttgtcg atacgaactt tgtagttacc ggttgcagca
     7801 acaacgttat caacagagct gttatccagt tcagtaccgt tgtctacgtc gccgttacca
     7861 ttgatagtca ggtgaccgtc agagttcata gcaacaatac cgtaaccgta gttagacttg
     7921 gaagtatccc acgtacggtc gttcaccaga tcagcgttca gaacgtaatc gctgctatgg
     7981 atatcgaaca caccagatac tacgttaccg ttatggtcag cgatgttgcc gctggtcagt
     8041 tccagtttgt cagtacggaa agcttcagta tcgaactgat caacgttcac atccaataca
     8101 ccaccgttag ttacggtgat agtattggcg tacaggttgt aatcagcccc ccacccagta
     8161 gattcggtca acgcaacagt actgtaggag ttaatagtca gatgatcggt atcaacgtag
     8221 ccatcttcac caatgttcag cgaagaaccg ttagttaaat cgatagtatc tgaagttaat
     8281 ttagattccg aaacattaac ctgagaacca ttattaactg tcaaggtatc aacgatggaa
     8341 cgacctgtag tatcccattc agaaccgtta ttcaacgcta cgttaaatac accgctctgg
     8401 aagaccttgt catcaacaac acgtttccag tcattgtttt catatgttgc atcaggagta
     8461 aggctgtttg tagcaacatc atacattgct gacgtttcag caacgttata aattgcctga
     8521 ccaacccact tgcttccgtt atcgagagtg agatttagtt catcaacgtt gaggctatca
     8581 tcagtccaac ccccgttggt atctttcacc ccgtcaccgt tggaatcatg accattcgga
     8641 tcaaaattac tattccaggt gctggtaaag gcaacatcac ccatcagcgt tgaattgctg
     8701 aggaatacat tgttcttcat cgcatagtcg gaacctgaag cagtaaagga cattgcaaca
     8761 tcacccgggc cagcataatc actcggttca tcagaattgc caaagtgacc agttttgcca
     8821 taagtattgc tatccaggat ataatttgaa cccgaagtca ctgtggaatc ttttacagag
     8881 atattgttat cccaagtatg agattcggta gtgtaggttt cgttgtaagt attaccttga
     8941 gataatgcaa tacctgcaac gcgggagtta ttggaaatat tgatgttact ttctacatca
     9001 agattaacag caacgccttc acttgtatca aatgtctcat ttgtgtgctt ggttacatca
     9061 ccatctttat aagaatcagt gaagtaaaga gcttcataat catcatcaat agtagtgtta
     9121 gcaatgttca aagtgaaaac atcaccatca cgccagtgtg tatcaacaac cgcatcagtg
     9181 taaaaatcat atccacccag attattacca tctgcatcaa aacgatcata ataaccgcca
     9241 ggcagcatag aagtaatcga accgtgaatc actgaattac tgatatcaag ctgattagca
     9301 gtactgtctg cataatcatg gttcaaataa taggttgaga ttgtcccgtt aacggtatcg
     9361 ccgttaaagg actgatcgaa atcaccattg gtgttgttgt aatagacaaa accactatag
     9421 tttacatagt tgccatctac agtgcctgcc ttatccatat gatcccaatc aagtgctgta
     9481 acgggattat cgatataagt ccaggtatca tagtttgctg cattagcctg agaggtgata
     9541 gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca ggagtgcatt
     9601 tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc atagaaaaca
     9661 tattatgttt cgcgctaacg attatgctgc gttacagacg aatggttcaa ttattctttc
     9721 ccctaagtcc gaatgtggac atatataatt tatggagaaa aacacgcatc aaattgataa
     9781 aaggacgata tttcaacatg ttgcagataa tgttattttt gatacatacg cgttataaat
     9841 atcaaaaaca caaaccagat aaatatctta tcaatataca acaaagtttg aattattcaa
     9901 tttatagcag cacaaccaaa cgccaaaaaa taataaccaa gagctataaa taaagattaa
     9961 aaatattttc agtgaatatg aaattattgc tatttctatt acaaagatgt cctggcctct
    10021 cttccattct cttcttgtca acatcgcgac aactttcgta aaacatccct accctgcttc
    10081 aggtatacta tgcccctcga ttccacaaac atcaggcaga ccatgacaga cttaatccaa
    10141 cgccctcgtc gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt
    10201 agccttaacg acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc
    10261 gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa
    10321 cgcatcgcca acgccggtat tcgttccgtg atgactttcg gcatctctca ccataccgat
    10381 gaaaccggca gcgacgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc
    10441 aagcagactg tgccagaaat gatcgtcatg tcagacactt gtttctgtga atacacttct
    10501 cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat
    10561 ttaggcaagc aagccgtggt tgcagctgct gcaggcgcag acttcatcgc cccttccgcc
    10621 gcgatggacg gccaggtaca ggcgattcgt caggcgctgg acgctgcagg ctttaaagat
    10681 acggcgatta tgtcgtattc gaccaagttc gcctcttcct tttatggccc gttccgtgaa
    10741 gctgccggaa gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt
    10801 cgcgaggcga ttcgtgagtc actgctggat gaagcccagg gcgcagactg cctgatggtt
    10861 aaacctgctg gagcgtacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg
    10921 attggcgcgt atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt
    10981 gctatagatg aagagaaagt cgtgctcgaa agcttaggtt caattaagcg tgcgggtgcg
    11041 gatctgattt tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaattt
    11101 ttttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc ccctcaccct
    11161 aaccctctcc ccaagggggc gagggaaccg tccgcactcg tatcaccccg cccgataaaa
    11221 cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca ggtaatcggc
    11281 attggcatag tgctgagtca cgcggttatc ccaaatcgca atatcattcg gttgccagcg
    11341 ccaacgcacc tgaaactccg gtttagtgat atgggcgaat aaaaagccta acaaggcttc
    11401 gctctctttc tcgctgacat cgacaattcg cgtagtaaag ccttcattca caaacagcgc
    11461 ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggag gatttttcgc
    11521 gaccgcctcg cgccagcgtt gatgttcctc ctcggttttg cggtatttgt attccgggaa
    11581 cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga agggaacaga
    11641 gagcgcctca taggccgcaa taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa
    11701 ctctttagct gccagaatcg cccctgcggg tggcgtttca ataaatgtca catcggtatg
    11761 ccagttgtcg ttatccggcg gattatcgtt atgggtatcc agcacgatga tctcgtcaac
    11821 cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct gggccagcgc
    11881 gcgttgctgc tgcggcgtaa tagtttgatc gcgcagaaac accacctgat ggcgcagcac
    11941 cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt cggcacccga
    12001 aatttgtgcg ccgatatacg gccccagcgg ggtaatgctc agacgttcac tcattgtact
    12061 tctccatgcc agggcgtcag acggcgctgt aacgcgcgca aacccagttc taaaagaaag
    12121 gcgataatcg caatcaccgc gattcccgcc agcaccacgt cagtcgcgag aaattcaccc
    12181 gctgactgaa ccataaatcc taaaccgcgc gtcgcggcaa tcagctccgc cgctaccagc
    12241 gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg cagcgcgccg
    12301 ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg ggcggcacga
    12361 atgcgaacct gctgcgcgct tttcaccccc gccagcgccg acatcgccac cggtgcaaaa
    12421 atcgctaaat agatcagtaa gatcttcgag gtttcaccaa taccaaacca gatcaccatc
    12481 agcggcaaat aagccagcgg cggcaccgga cgataaagtt cgattaccgg atccagaatg
    12541 ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg gaataccgat caccaccgcc
    12601 gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg
    12661 tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacttg ttgcagcggc
    12721 ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg tccaccatac agtgagtaaa
    12781 accgctaacg tgccaatgct taaggtcacc tgacgcgaga gcggccagcg ccatttcagc
    12841 cgccgcgaat gcagtttttc attaatgagc acactcatga gaacgcctcc cgttgctcaa
    12901 atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc
    12961 ggctcgactc tcccgcaaca aagcggcgag caaagttgag cggcagccgc tccagcacac
    13021 ggccagggcc ggatgaaagc agaaccagtt cagtcgccat aaacaccgct tcttctatat
    13081 cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc agcagagttt
    13141 gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg ttcgtcgagt aataacagct
    13201 ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg acgttgacca ccggaaagct
    13261 gccagatata gcgtttttct gcgccttcca gccccacttt tttcagcatc tggtgcgcga
    13321 tttccagtcg ctgcattttc tctatacctg ccaattgcag gccgaacgcc acgttgtcct
    13381 gtacattgcg ccacggtagt agcccttcat tctgaaaaac tacgccacgc tctgctcccg
    13441 gtccctcaat acgcttaccc gccagtagaa tgctgccatg ctgataaggc acaaaaccgg
    13501 caatcagatt cagcaacgtg gttttaccgc agccggacgg ccccagcacc accagtagct
    13561 cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca taatcggcgt
    13621 aaagatgaga gatttgcagc atctgcgcct ccttttattg cacgaagcgc gaggtaacgt
    13681 actggctgta atcattcgct acagccggga ccttgccctg ctctttcaaa aactgcgcgg
    13741 tgtcgatgat cgctttgttc accggtccgg tcagttctgc cgtttgttgc tgcggcgtca
    13801 gataggtatt ccccttcacc agccccggaa cgtcaccttc aggcacgccg cttaaacgcg
    13861 ccagtttgct gatgttttcc ggctgtttca gccacacgtc tgggttagca atgtacggtt
    13921 gctgagcatc gatggcgctt ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa
    13981 aatctttgcg caccacccag acgtccagcg ttggcgcgcc ccactgcccg acctgttcag
    14041 aatcggtcaa caccttgccg tctttttcca gggcgttaac cgccggtgcc cagacataag
    14101 caccatcaat atctccccgc tgccaggcag cgataatcgc gggcggctgc aggttcacaa
    14161 tctccacttg cccgggttta atgccccagt gtttcagcgc cgccagcagg ctgtagtggg
    14221 tggtggagat aaacggtacg gcgatgcgtt tgccaatcag atcttccggt ttgctgatag
    14281 ttttctttac caccagcgct tcggagttac ccagttttga cgccagcaag aagacttcaa
    14341 tcggcacctg ttggctggct gcaaccgcta acgggctgga accgaggttg ccgatttgca
    14401 catcgcctga agccagcgcc cgcacgatgc tggctccgct gtcaaactta cgccagtcca
    14461 cgctcgcccc gctctcttta gcaaaggtgt tgtcggcctg agccactttc gccggttcgg
    14521 ctgaggtttg ataggccacg gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca
    14581 gtgcggctag aagtgtgtta cgcgatgaaa ttgccatgat tgtctgctcc cctgtcttgt
    14641 tatgggagca gtattcagga ataaaaacat tcattaaaag aattagtcgt tatcgcacag
    14701 atgattttat tcttagcaaa aaaacggcta aggaaagatt aaatatgcaa ttactccgtt
    14761 ctaacaattg ggaaaattac tacacttcat tttagtgatg cataaacaac caca
//
LOCUS       DAMZII010000113        14734 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZII010000113 DAMZII010000000
VERSION     DAMZII010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.113"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..780
                     /locus_tag="POA77_003545"
     CDS             <1..780
                     /locus_tag="POA77_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HDI6366915.1"
                     /translation="SRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLD
                     IKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHD
                     SYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEM
                     TVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIR
                     HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1049..3286
                     /gene="nrfB"
                     /locus_tag="POA77_003546"
     CDS             1049..3286
                     /gene="nrfB"
                     /locus_tag="POA77_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDI6366916.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMSCVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3273..6245
                     /gene="nfrA"
                     /locus_tag="POA77_003547"
     CDS             3273..6245
                     /gene="nfrA"
                     /locus_tag="POA77_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDI6366917.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6246..7136
                     /locus_tag="POA77_003548"
     CDS             6246..7136
                     /locus_tag="POA77_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDI6366918.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAAHQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7319..8080
                     /gene="envY"
                     /locus_tag="POA77_003549"
     CDS             7319..8080
                     /gene="envY"
                     /locus_tag="POA77_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDI6366919.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(8126..8202)
                     /locus_tag="POA77_003550"
     tRNA            complement(8126..8202)
                     /locus_tag="POA77_003550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8166..8168),aa:Arg,seq:tct)
     gene            8445..9077
                     /gene="fimZ"
                     /locus_tag="POA77_003551"
     CDS             8445..9077
                     /gene="fimZ"
                     /locus_tag="POA77_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDI6366920.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(9080..9595)
                     /gene="sfmF"
                     /locus_tag="POA77_003552"
     CDS             complement(9080..9595)
                     /gene="sfmF"
                     /locus_tag="POA77_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDI6366921.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELHNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(9606..10613)
                     /gene="fimH"
                     /locus_tag="POA77_003553"
     CDS             complement(9606..10613)
                     /gene="fimH"
                     /locus_tag="POA77_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDI6366922.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(10626..13234)
                     /locus_tag="POA77_003554"
                     /pseudo
     CDS             complement(10626..13234)
                     /locus_tag="POA77_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(13265..13957)
                     /gene="fimC"
                     /locus_tag="POA77_003555"
     CDS             complement(13265..13957)
                     /gene="fimC"
                     /locus_tag="POA77_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDI6366923.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(14177..14719)
                     /gene="fimA"
                     /locus_tag="POA77_003556"
     CDS             complement(14177..14719)
                     /gene="fimA"
                     /locus_tag="POA77_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6366924.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFKGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3928 a         3633 c         3521 g         3652 t
ORIGIN      
        1 agtcgccgca ggggagcgat tcatgtcatt agtgcgttct caacaatgca cagtctggtc
       61 atcggacaga tcaagacgga tgagaaatct aatgagatta cagctatccc agaacttctt
      121 aacatgctgg atattaaagg aaaaatcatc acaactgatg cgatgggttg ccagaaagat
      181 attgcagaga agatacaaaa acagggaggt gattatttat tcgcggtaaa aggaaaccag
      241 gggcggctaa ataaagcctt tgaggaaaaa tttccgctga aagaattaaa taatccagag
      301 catgacagtt acgcaattag tgaaaagagt cacggcagag aagaaatccg tcttcatatt
      361 gtttgcgatg tccctgatga acttattgat ttcacgtttg aatggaaagg gctgaagaaa
      421 ttatgcgtgg cagtctcctt tcggtccata atagcagaac aaaagaagga gccagaaatg
      481 acggtcagat attatatcag ttctgctgat ttaaccgcag agaagttcgc cacagcaatc
      541 cgaaaccact ggcacgtgga gaataagctg cactggcgtc tggacgtggt aatgaatgaa
      601 gacgactgca aaataagaag aggaaatgca gcagaattat tttcagggat acggcacatc
      661 gctattaata ttttgacgaa tgataaggta ttcaaggcag ggttaagacg taagatgcga
      721 aaagcagcca tggacagaaa ctatctcgcg tcagtccttg cggggagcgg gctttcgtaa
      781 tcttgccctg cctgctcacc tcctccaggc acttccacta cattatgacc tgctcggtgc
      841 caccgctcct tttcactatg acgttaacct cccctccttg acaccatgct tttgtctaca
      901 ttctacgcaa acggcaactt ttataaccag tgtaaaaata acgtgccgca atagtcatac
      961 ctattaatgg taaaaagctg tcacaattca taaaaaacct taatatacgc caccctaaac
     1021 ataaccagcg ttaatgtaag gtttttgtgt ggactggctt cttgatgttt ttgcaacctg
     1081 gctctacggc ttaaaagtaa tcgcgataac gttagcggtc atcatgttca tcagcgggct
     1141 ggacgatttt tttattgatg tcgtctactg ggtacgccgc attaaacgca agttgagtgt
     1201 ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat aaaccagatg aaaaaccgtt
     1261 agcgattatg gttccggcgt ggaatgaaac gggcgtcatc ggcaatatgg ccgagctggc
     1321 ggcgaccacg ctcgactacg aaaactatca tatctttgtt ggcacctacc ccaacgaccc
     1381 cgatactcaa cgtgatgttg acgaagtgtg cgctcgcttt ccgaacgtgc ataaggtagt
     1441 ctgcgcgcgt cctggtccca ccagcaaagc cgactgtctg aacaacgtgc tggacgccat
     1501 cacccagttt gaacgtagcg ccaatttcgc ttttgctggt tttattctgc atgacgccga
     1561 agatgtgatt tcaccgatgg aattgcgtct gttcaactat ctggtcgagc gtaaagatct
     1621 gattcagatc ccggtgtatc cgttcgaacg cgaatggacg cacttcacca gcatgactta
     1681 cattgatgag ttttcagagc tgcatggcaa agatgttccg gtgcgtgaag ccctcgccgg
     1741 acaagtgccc agcgcaggcg tcggcacctg tttcagccgc cgcgccgtga ccgctctgtt
     1801 agctgacggt gacggtattg ctttcgacgt gcagagtctg actgaagatt acgacattgg
     1861 tttccgcctg aaagagaaag gtatgacgga aatttttgtc cgttttccgg tggtggacga
     1921 agccaaagaa cgcgagcagc gtaaattttt acagcacgca cggacgtcaa acatgagctg
     1981 cgtgcgcgaa tatttccccg ataccttttc gactgcagtt cgacaaaaat ctcgctggat
     2041 catcggcatt gttttccagg gctttaaaac ccacaaatgg acctccagcc tgacgctgaa
     2101 ctactttctc tggcgcgacc gcaaaggggc aatcagtaac tttgtcagct tcctcgcgat
     2161 gctggtgatg ctccagcttt tgctgttgct ggcgtatgaa agtttgtggc ccaatgcctg
     2221 gcatttcctt tctattttta gcggcagcgc atggttaatg accctgctgt ggctaaactt
     2281 tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc gtcactggct actacggtct
     2341 gacgcagggg ctgctatctg tcctgcgtct tttctggggc aacctgatta acttcatggc
     2401 caactggcgc gcgctaaaac aggtacttca acacggcgat ccacgtcgcg tcgcgtggga
     2461 taaaacaacg catgacttcc ccagcgtgac tggcgatacc cgctcgttgc gcccgttagg
     2521 ccaaattctg ctggaaaatc aggtcatcac tgaagaacaa ctcgatacag cactgcgtaa
     2581 tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg caggggctga ttagcgccga
     2641 gcagctggca caggcgctgg cagagcaaaa cggcgtggcg tgggaatcca tcgatgcctg
     2701 gcagatcccg tcctcgctga ttgccgaaat gccagcctcc gtagcgctgc attatgcagt
     2761 actgccgctg cgtctggaaa atgatgagtt aattgtcggc agtgaagatg gtattgaccc
     2821 ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa gtgcgttacg tcattgttct
     2881 gcggggacaa attgtcacgg ggttacgtca ctggtatgca cgccgacgcg gtcacgatcc
     2941 gcgggcaatg ttgtacaatg cggttcagca tcagtggctc acggaacagc agaccggtga
     3001 aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc gaaatactga ccacgctcgg
     3061 tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc catgaacgca gttctctgcc
     3121 gctcggcaag tttttggtca ccgaaggcgt tatcagccag gaaacgttgg atcgcgtcct
     3181 gacaattcaa cgcgaattac aagtttcgat gcaatcacta ttactcaaag caggtttaaa
     3241 cacagaacag gttgcgcaac tggagtccga aaatgaagga gaataacctt aatcgcgtca
     3301 tcggatggtc tggtttactg ctgacgtctt tattgagtac cagcgcactc gcagacaata
     3361 tcggcaccag cgcagaagag ctggggctga gcgattatcg ccattttgtt atttatcccc
     3421 gtctcgataa ggcgctgaag gcacagaaaa ataacgacga agcaaccgcc atccgcgaat
     3481 ttgaatatat acaccagcag gtgccggata atattccgct gactttatac cttgcggaag
     3541 cctatcgcca ttttggtcat gatgaccggg cgcggctgtt gcttgaggat caactgaaac
     3601 gtcacccagg agatgcccga cttgagcgca gtctggcggc tattccggtt gaagtgaaaa
     3661 gcgttacgac tgttgaagaa ctgcttgccc agcaaaaagc gtgcgacgct gcgccgaccc
     3721 tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct ggcacaatta cctgtcgcca
     3781 gagcgcaact gaacgatgcg acgtttgctg catcgccaga aggaaaaacg ctgcgaaccg
     3841 atctgctgca acgggcaatc tacctgaaac aatggtccca ggcagatacg ctatacaatg
     3901 aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg tcagtggttt gacgtgcttc
     3961 ttgccgggca gctggacgat cggatcctgg cactgcaatc acaggggatc ttcaccgatc
     4021 ctcagtcata tattacttac gcgaccgcgc tggcttatcg tggcgaaaaa gcacgcctcc
     4081 agcattatct caatgaaaat aagccgctgt ttaccacgga cgcacaagag aaaagttggc
     4141 tctatctgtt atctaaatac agcgccaacc ccgttcaggc attggcgaat tatacggtgc
     4201 agtttgccga taaccgccag tatgttgttg gcgcgacgct accggtgctg ttaaaagaag
     4261 gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc cgccaatgaa atgcttgagg
     4321 agcgttatgc tgtcagcgtg gcgacccgta acaaggctga atctctgcgt ctggcacgat
     4381 tgctgtatca gcaagaaccg gcaaatctta cccgcctgga tcaactgacc tggcaactga
     4441 tgcagaacga gcagtcacgc gaagctgccg atttattgct gcaacgctat cctttccagg
     4501 gcgatgcgcg tgtcagccag actttaatgg cgcgactggc gtctctgctg gaaagtcatc
     4561 cttacctggc aacgccggcg aaggtggcga ttttatcgaa acccttaccg ctggcggagc
     4621 aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa ttgcccggca atagttcgct
     4681 tgctgggcga tatgtcgcct tcctacgatg ctgccgcctg gaaccgtctg gcaaagtgtt
     4741 atcgggacac gctacccggt gtggcgctgt atgcatggct tcaggccgaa caacgacaac
     4801 cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta tcaggttgag gactacgcca
     4861 ccgcactggc ggcctggcag aaaatcagtc ttcacgacat gagcaatgag gatctgcttg
     4921 ctgctgccaa taccgcccag gcggcaggaa atggtgcagc tcgcgatcgc tggctacaac
     4981 aggcagaaca acgtggactg ggaaacaatg ccctctactg gtggctgcat gcgcaacgtt
     5041 acattcctgg tcagccggaa ctcgcactga gcgatctcac gcgctcaatc aatattgcgc
     5101 cttctgccaa cgcttacgtt gcgcgggcga caatttatcg ccaacgtcat aatgtcccgg
     5161 cggcggtgag tgatttgcgc gccgcgctgg aactggaacc gaataatagc aacatccagg
     5221 cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc acagtcgcgg gaaatgctcg
     5281 aacaggcgca taaagggctt ccggacgatc cggcactgat ccgacaactg gcctacgtga
     5341 accagcgtct ggatgacatg cctgcgacgc agcactacgc ccggctggtg attgatgaca
     5401 ttgataatca ggcgctgata accccactga ccccagagca aaatcagcaa cgcttcaatt
     5461 tccgccgttt gcatgaggag gtcggtcgcc gctggacgtt cagtttcgat tcttctatcg
     5521 gcttgcgttc cggggcaatg agcaccgcta acaataatgt cggcggcgca gcgccaggga
     5581 aaagctatcg tagctacggg caactggaag ccgagtaccg catcggacgc aatatgctgc
     5641 tggaaggcga cctgctctca gtttatagcc gcgtctttgc cgataccgga gaaaacgggg
     5701 tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag ccgctgcgcg
     5761 atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacgggcaa aatggcgcat
     5821 ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac agcgacgaat
     5881 ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg gcgcaatata
     5941 tccgccagga tattcaggcg tggacggcag attatcgcgt cagctggcat cagaaggtag
     6001 ctaacggaca gactattgag ccttacgctc acgttcagga caacggctat cgtgataaag
     6061 gcactcaggg cgcgcagctt ggcggagtcg gggtccgctg gaatatctgg accggcgaga
     6121 cgcactacga cgcctggccg cacaaagtca gtctcggcgt cgagtatcaa cataccttta
     6181 aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt ggagtgcact
     6241 ggtaaatgcg taagttcatt ttcgtattgc tgacactgct tttggtcagc cctttttcct
     6301 ttgcgatgaa aggtattatc tggcaaccac aaaaccgaga tagtcaggtt accgataccc
     6361 agtggcaggg gctgatgagt cagttacgtt tgcaaggctt cgataccctg gttttgcaat
     6421 ggacccgtta cggcgatgca tttacccagc cagaacagcg cacgttattg tttaagcgtg
     6481 ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg gctgaacgcc gatccggaat
     6541 tttttatgca ccagaaacag tcgtccgcag cgctggaaag ctatcttaat cgcctgctgg
     6601 ctgccgatct ccagcaagcc agattatgga gcgccgcgcc tggcataacg ccggatggct
     6661 ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagctgcc catcagcctt
     6721 tgctaacatg gttaaacaac gcgcagcggc tgattagcga tgtttcagca aaaccggttt
     6781 atatcagtag ttttttcgcc ggaaacatgt cgcccgatgg ctatcgccaa ctgctggaac
     6841 acgttaaagc aaccggcgtt aatgtttggg tacaggatgg cagcggcgtg gataaactga
     6901 ccgctgaaca gcgtgaacgt tatctacaag ccagcgccga ttgccaaagt tccgccccag
     6961 caagcggcat tgtttatgaa ctttttgtcg ccggcaaagg caaaaccttt acagcgaaac
     7021 cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg ttcctcttgc ggtaaagaca
     7081 ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg cattctcgag tattaaatct
     7141 cctccaggta agtcgggtac gacctggctt acctctttcg ctctcgtcag aattccatca
     7201 agattaattg ctaaaaagcg tgctaaagaa agaagatcgt tatcggcgaa cgggccgatc
     7261 actcatgcag gaagcataga tcctttgagc ggagtttctg atatctgagg agtgcgaaat
     7321 gcaattgagc agcagtgaac cttgcgtggt gatcctgacc gaaaaagagg tagaggtaag
     7381 cgtcaataac catgctacgt ttacccttcc gaaaaactac ctggccgcct tcgcgtgcaa
     7441 caataacgtc attgaactct caacgttaaa tcacgtatta atcacccaca tcaaccgtaa
     7501 catcatcaac gattatctgt tgtttttaaa taagaactta acctgtgtaa agccctggtc
     7561 gcggctggca accccggtta ttgcttgtca tagccgtaca ccggaagtgt tccggctagc
     7621 cgccaaccac agcaagcagc aacccagcaa accctgcgaa gcggagttga cgcgcgtatt
     7681 gctttttacc gtattgtcta actttcttga gcaatcgcgg tttattgccc tactgatgta
     7741 tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt attcaaagcg atattcagca
     7801 ttactggaat ctgcgaattg tcgccagttc gctatgttta agccccagcc tgctcaaaaa
     7861 gaaattaaaa aacgaaaata ccagctatag ccagattgtc acagagtgtc gtatgcgtta
     7921 cgccgtacag atgttattga tggataacaa aaatatcact caggtggcgc aattatgtgg
     7981 ctatagcagc acgtcatact ttatctctgt ttttaaggcg ttttacggcc tgacaccgtt
     8041 gaattatctc gccaaacagc gacaaaaagt gatgtggtga agggcaaagc ggaaacggat
     8101 aagacgggca taaatgaaga agaaatggcg cgccctgcag gattcgaacc tgcggcccac
     8161 gacttagaag gtcgttgctc tatccaactg agctaagggc gcgttgatac cgcaatgcgg
     8221 tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca atggcttttg atctggttgc
     8281 tgaacaagtg aacgaccgcg tctgattttc tgatttattt cgctatagcg gcaaacaaac
     8341 acacaccgct gcgcgtctaa atcaagaaaa cccgtatttt catgtatcaa agtacaattt
     8401 accgccctaa cggaaaattg tccgctccta tgagactggt aactatgaaa ccaacgtcgg
     8461 tgatcattat ggatactcat cctatcatca gaatgtctat tgaagttctg ttgcaaaaaa
     8521 acagtgaatt gcagattgtc ctgaaaacgg atgattaccg cataaccatc gattatctcc
     8581 gaacccgtcc tgttgattta atcattatgg atatagactt gcccggaaca gacggtttta
     8641 ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa agtgttattt ttatcatcga
     8701 aatcagaatg cttttatgct ggcagagcga tacaagctgg tgctaacggt tttgtcagta
     8761 aatgcaatga tcagaatgat atttttcatg ccgttcagat gatcctctcc ggatacacgt
     8821 tttttcccag caaaacgctt aactatataa aaagcaataa atgtagtacg aatagttcaa
     8881 cgatcactgt gctatctaat cgtgaagtga ccatattacg ttatctggtt agcggattat
     8941 ctaataaaga aattgccgat aagttattac ttagcaataa aacagttagt gcgcataaat
     9001 ctaatattta tggcaagcta ggtttgcatt caattgtaga gcttatcgac tacgccaaat
     9061 tatacgaatt aatataatat taattataat tgatcataaa tatcgcatcc gctttcgcca
     9121 cacctggccg aacaccgctg gctaacgctc gataattggc aaaaaagttt agtgtgacat
     9181 tgccatttgc atctacttcc tcagtcgggc tcgcctcccc cagtgcgagc cgggagcgat
     9241 cgctattatg taattcgatg gcgacggttt gtgccattgc gggatcatcc agagccagca
     9301 ggttggtatc ggatgccggc gttcccgtaa ataaaatcgc aactgaaccc ggaggacatc
     9361 cctccagccg caggctaaaa gggacgagtg ccgtggtatc gccagcgttc agtaattgtg
     9421 tcgtaggcca tctgcctaaa tctaccgtct tatcaatatc cgcagtgttt acggtacagg
     9481 agaaatcaac aacgttaccg tgcaaattga tattaatcgt tcccaaaggg tcagctgccc
     9541 atccactgga actccacagt aacccgcaga aacagctaaa gagtgctctt ctcattctcc
     9601 gtacctcatt gataatcgac gcgtaaatac cccagcgcgc taaacggccc ttcggtcggt
     9661 ttttgaccgg taatactgat aggccaggcg cgaagtgtga cattggctgc cgcagctgca
     9721 tccagacgga aaggaataac gctattgaga tcgttaggcg tgatcggcgt atcgttctga
     9781 tcggcgacaa taaaacctaa atcctgattg tccgacacca tcgcctgacc agaaacggca
     9841 ctggcttcca gacgcattgt taaataagcc tgcgcagcaa cattcgtaca tttgaccgca
     9901 atgctcttgg tttgcggcat gacaccagca ggtcgattac ccggccctgc cgcactaaat
     9961 aacgatgcgc cgatatcacc aaaatcaaat tcaacaatct gcccggcatt taattcgcag
    10021 ttttgcggta cttcaacccg gccaccaaaa ctaatggtat aaacaggtgt aaccaacgga
    10081 tcgtaaggcg ttgatgtcac atacacggta aacatcacct gtctggggat ctccaccatg
    10141 ttaataaagg gacgaataac ctttaaacga aatattaaac gagaatccat caccccgaat
    10201 ggaaggtttt gcgaaacgtt aggatctgtt cccatcagaa tataattttg cggggggaac
    10261 tgaatatcca tcacactatc aaccagactc atcgcaccta atagataatc atttaattgc
    10321 atatatttat aattatcgat agtttcctga acaataaagt tggtgacata actacggtat
    10381 gtataattca ccagcgtacc gggtggacaa attgctgaga cacctaccca gccggatttt
    10441 tccggtaaaa caacaatctg ccctggctgg ttattggtgc tattaaagat atttgagaga
    10501 tcatagtgtt catcttctgc tacgccagta ctatttgcac aaacagtagc ccaggatatg
    10561 tttgccagac acaaacatgc catcgccaat aataatctgc agattatctt cattgataat
    10621 ttcctttatg agccaggatg tgtgcaaact gccgaaataa cagtgacggc ttgctgtaag
    10681 ctttgcttcg gcaatacata gtgcgcaatg cagcgtgagt tcgcgccgtc tccccactga
    10741 accagtaatt caccagacaa tggcgcacca cttaaataaa cttgcccgtc atcacccaca
    10801 tactggttat tccgatactg ttttcccgta ccagtgatcc aaacggcacc ggttttccgc
    10861 cctgggtaac ggtaatgagc gcccgcacgc ctatgcgggt atcaaaattg gcacgaacca
    10921 acgcgccttg cgtcggcaca acgctgctaa tatttttttc aacatcgatg gaattcccca
    10981 tcgtattggt atcaagcgcg atacggttat accgataaac cgtggcatac ggcatcaccg
    11041 catagccgcg ccagtcggtt aaaatgccag tttgattttc aatgcgtaca ccgcctgcgc
    11101 caggcgcttt aatcaaaaca ttggtatccc ctaaaggctg gctcagcgtt atgccatttt
    11161 catgcccgac cacaccgcca gacagctgcc agttaacgtc atgttgatcg cggtcgtagt
    11221 tatacccgac ccccagcgta gcgtaagcgg cctgccagtt tgccgtggcg ctgcccgtat
    11281 acccattatt cgaggtatca ccctggctta cgtgataact caggttgtgg ccttccagta
    11341 aggtcccacc tacacctgcc agccagctat tttgcccgtt gctgttacgg ttggcgttaa
    11401 aggaggcata agcgcggtcg agcgcatttt cccgggtgta gcgacgtttg gtcagaacat
    11461 tgaaaggcac tgaaacatta agtccgacaa tacgttcgtt atcggggatc cctacagatt
    11521 cattccacga aaatgagagt gaataactga tgccaaccca gctgctggta taccccacct
    11581 gataccaggt atctgaatcc gaagtattcc agtatttttg atgggtacca gagatataaa
    11641 gcgagccaaa gtcattaagt gattgtgaaa tatttaactg caaacggtct ttacggctaa
    11701 agcgtaaatt gtggtaattc acgattatcg gttcatcccg atgctcaccg tcgtaatcgt
    11761 aatcatattc gtacccctcc attcgacgat acgcaacatc atcaagggta taaaaacctt
    11821 gtgtcgaata gcggtaaccc attaactgaa aattggtgcc gaaggtgttc atcgatttcg
    11881 catagaggaa gcgaatggaa tccccctcat gacgactgtc gtcggctaac tgactgcgcg
    11941 catgggtcac atccagcgac actgcccccc agtttccgag attgcgcccc agtcctaata
    12001 aaaaggcggt gtaattggca gataattgcg tcccgccgta ggcagtaaat tcctgtggta
    12061 aaccgccgag tgccgtgccc tgaaaaaaga aaggtgatga ttgctgacta ttaccgctac
    12121 gaaaatcgcc cgccgtcagg tcaaatttga aacgcccttc gcgttgtaaa atcggcactg
    12181 ttgaatacgg aattgtgtaa ttctgctgat tgccatcgcg ctcgtcgatg gtgacatcca
    12241 gatcgccatt tgaagatgtc gggtgcaaat ctgtaatttc aaaagcgccg ggggaaacat
    12301 agctttgata gataataaaa ccattttgcc gaatcgttag ctgggccgcc gtacgggcaa
    12361 tcccacgtac cgttggggca aatccttgct ggctatcagg atacatatta tcggaagagt
    12421 aaagccgtat accacgaaat ccaacaccat cgaaaatatc acttcctgta ttgccgtctc
    12481 ccattaccag ttcacttttc agcggaataa tggcgcgctg tacccaggtg ccaatattat
    12541 tccactgctc tgaatgatat ccatttccgc gaaaatagtt ccaggaacca ttgttgcgta
    12601 aacgccacgg gccaatatta atcccgctga gttcactaaa aaaatagcta tcgttaccat
    12661 tacctctgtt accggtgaaa ttataattca ggagtaaagc aggaatacct tcatcccact
    12721 cttcaggcgg aatgtgaccg tgagcgctac tcaacaacgc tatctgagga atagtgatgt
    12781 tcaggcgcat cgctgcaaaa tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat
    12841 tgatgcattt attgttttgc tgctgggcta actcgggaaa tgcactgcta ttaaggccaa
    12901 ttcgttcaag gagtacctgg ttaaaacagg gcattaaccc acctgattta tcacctgtat
    12961 tttctgttgt cgattcaaat acgatatcct gtgaaccaat gaactcatca ttacgccaga
    13021 gatccacccg atacactccc gcaggttgat gattgcctct ctcaaagcgc gataaatcag
    13081 caacggatgt gccattctct aataaaaatg ccgggttgaa ataactttcc gcatagctta
    13141 aaaaggaggg taaaatggca agagatgaat aacaaattcc ggccagacac caggtatacc
    13201 tatgcggaat atccgtagta gtgggtattt tcataattag ctctgatatt gtcagatgat
    13261 tcggttagtt taagttcact tctctgaccg gggttactga accataatca ttaacggtct
    13321 gaaatttcag ctttccctgc actcctgagg gtaagggaat ttgtgaacta actctgggtg
    13381 ccgccatagc attccccaac ttttggctgc ccacttgcag gttaacgagg gtgacataaa
    13441 aaggtgatgg attatgaaca ttgatatagt taccggatcg cgaaaacttg agtgtgtccg
    13501 gcgcttctgc gggtaattct tgtaattgaa ttggacggag aaataatttc atgcgcgata
    13561 aaatcgccag ttgcaaaaca ttcctgccgt tcaatgcatt tttatctacc gaagggatcg
    13621 tcttaacatt catccagaac agagactcac gatctgccgc cagcggtgga ccggtgtaaa
    13681 taatacgcaa ggtattttcg cttttgggtt cactaacaaa cagtggcggt gtaatgatga
    13741 atgacttttc ttttacaccg ctgctgtttt caatccacga attgaccaga aagcgctcat
    13801 tggtatggct atttctaatc cataccgcag tctgtttagc atcagcggga taaataatac
    13861 gcgtggcacc taatgcgatc cctccggcag catgggcgct ggccgaaatg attaaataaa
    13921 atataatgag catcaataac tttattttag tcatcatagt taatacgcct tgtgtattcc
    13981 tgacggcaaa aataagaaat ataaagaaat gattgctttg gtttcttaca acacatcatt
    14041 cccttttcat ttaataaatc ggcatgatac aaagtgaaca tgatttcgat ttacattgcc
    14101 accataccgc ttcagacaac cggtacatta ctttgttata agacgtgata tataattcaa
    14161 aacaacgtgg ttttgattat tcgtatctca taataaaagt cgcgtcagca tttgcttgcc
    14221 ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc
    14281 cggggatcaa gttctggttg gttgagaagc tattcccatc aggtttgaga attgcggatg
    14341 tattatcaag tatttcaata ccgacacccg ttgctgttgt tgtattggta ctggatgcaa
    14401 tggccaataa attatcattg attgcatcag cctgaccaga aaatgcaacg gcagcattag
    14461 cggcaacaac aggatcgcag tcgttcaact gaatggtgaa tggaattaat gcagaggtat
    14521 taccaacagc attgaaaata tcggtacgat attgaccgag tgtgacaacc tggtctgccg
    14581 agtcagtatt cactgaacag gcagcattca ccagttcgcc tttaaagtga attgtaccgc
    14641 catctacaac ggcagcataa ctacccgtag cggcgaatag tgcggcagcc agcgcagacg
    14701 aaataaatct taatttcata tatattcctt caat
//
LOCUS       DAMZII010000114        14619 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZII010000114 DAMZII010000000
VERSION     DAMZII010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.114"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            71..427
                     /locus_tag="POA77_003557"
     CDS             71..427
                     /locus_tag="POA77_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6366925.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(478..1374)
                     /gene="yhaJ"
                     /locus_tag="POA77_003558"
     CDS             complement(478..1374)
                     /gene="yhaJ"
                     /locus_tag="POA77_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDI6366926.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1479..2180
                     /locus_tag="POA77_003559"
     CDS             1479..2180
                     /locus_tag="POA77_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDI6366927.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQARAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2203..2367
                     /locus_tag="POA77_003560"
     CDS             2203..2367
                     /locus_tag="POA77_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366928.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2501..3811)
                     /locus_tag="POA77_003561"
     CDS             complement(2501..3811)
                     /locus_tag="POA77_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDI6366929.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3839..5170)
                     /locus_tag="POA77_003562"
     CDS             complement(3839..5170)
                     /locus_tag="POA77_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6366930.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5445..6809)
                     /gene="tdcG"
                     /locus_tag="POA77_003563"
     CDS             complement(5445..6809)
                     /gene="tdcG"
                     /locus_tag="POA77_003563"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI6366931.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6881..7270)
                     /locus_tag="POA77_003564"
     CDS             complement(6881..7270)
                     /locus_tag="POA77_003564"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDI6366932.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7284..9578)
                     /gene="tdcE"
                     /locus_tag="POA77_003565"
     CDS             complement(7284..9578)
                     /gene="tdcE"
                     /locus_tag="POA77_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDI6366933.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9612..10820)
                     /gene="tdcD"
                     /locus_tag="POA77_003566"
     CDS             complement(9612..10820)
                     /gene="tdcD"
                     /locus_tag="POA77_003566"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDI6366934.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10846..12177)
                     /gene="tdcC"
                     /locus_tag="POA77_003567"
     CDS             complement(10846..12177)
                     /gene="tdcC"
                     /locus_tag="POA77_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDI6366935.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12199..13188)
                     /gene="tdcB"
                     /locus_tag="POA77_003568"
     CDS             complement(12199..13188)
                     /gene="tdcB"
                     /locus_tag="POA77_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI6366936.1"
                     /translation="MHITYDLLVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13287..14225)
                     /gene="tdcA"
                     /locus_tag="POA77_003569"
     CDS             complement(13287..14225)
                     /gene="tdcA"
                     /locus_tag="POA77_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDI6366937.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14540..>14619
                     /locus_tag="POA77_003570"
     CDS             14540..>14619
                     /locus_tag="POA77_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDI6366938.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSR"
BASE COUNT         3819 a         3888 c         3390 g         3522 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttagccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtttaga gtttccattc ctttctctca tttacctggc
      241 agcaacaatc attccggtca ttgctctgtg tgttcgtcgt ctgcacgata ctgaccgttc
      301 aggtgcgtgg gcgcttctct atctggtacc gatcattggc tggttagttc tgtttgtatt
      361 tgcctgttta gaaggcaatt ctggcagcaa tcgttacgga aacgatccta agtttggttc
      421 aaattaatct tagaattggg gcgacatttc gccccttttt attaataata acaaatatta
      481 ttttccgcta aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact
      541 gtcacgacgc caggccataa taatatcgat ctcgctggtc gattccgggc tgacgacacg
      601 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
      661 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
      721 gtccagcagc tgtacggtca acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc
      781 acgatatttc acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
      841 aggcgcggca acgtagacgt tcattaaggt atagagcttg cgcgagttga tctccgacga
      901 ggaacgaaaa tgcatatccg gcgcgataac gatatccgcc cgcccctgct ccagtcgttc
      961 ccacgccccc gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag
     1021 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcctcggtca caatggtgag
     1081 atgcgtttcc caaccgcgcg cgagggcttc cgcatcggta gtcagtttat ctgcggcttc
     1141 cagcaaaacg cgccctcgct ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg
     1201 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
     1261 aagtgcggaa ggcacgcgtc ccagctcatc cgctgccgcc gcaaaactgc cccggcgatc
     1321 gatcgcatcc ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt
     1381 ctcattcagg aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc
     1441 cccttaccat aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca
     1501 gacagtgtgg acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac
     1561 actacttcga cccgaaattg ttaggctatg cctccctgcg cgtgcttaac caggaagtgc
     1621 tggccccagg tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga
     1681 ttctggatgg agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagagccg
     1741 gagaggcgtt gctgctctct acccagccgg gtgtcagcta tagcgaacac aatctcagca
     1801 aagacaaacc gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc
     1861 cgctgattca aaaactggcg cttaatatgg gcaagcagca attaatcgcc tcgccagagg
     1921 gcacagtggg tagcctgcaa ctgcgccaac aagtgtggct gcaccatatc gtgctcgaca
     1981 aaggcgaaag tgcgaatttc cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg
     2041 ggaaatttca tgcgcttacg catcatgaag agaaagcggc gctaacctgc ggtgatgggg
     2101 cgtttattcg tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc
     2161 tgatagattt gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaattggcc
     2221 aaaaagcgcc agccggtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat
     2281 tttggctatg aagagatgtt gagcgagctg gaagctatcg tcgcggatgc tgaaacgcgt
     2341 ttagccgagg atgaagctac cgcgtaaaga caggttgccg gatgcggcga aaacgcctta
     2401 tccggcctac aaaatccaac aacattaacg aattaaacaa cccgcaggcc ggacaagatg
     2461 cgccagcatc gcatccggca cgatacccaa aacctggcgt ttatctggcc ttgctcgcca
     2521 taatctcgat aatctgccga tcggtttgct gcatcgaatg gcttgctaac gcacacaggt
     2581 tggcaatcga ctgctcaaca tcatgcgcca caatcccttc attgccggtc acggcggtat
     2641 catccagcgc cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg
     2701 cgcagctgtt cgacgctcca tcgcaaatca tgccgctgac atcgccgatc atactgctga
     2761 tcgccatcga gatggtttca taacgcccat ccaccagcca tgccatcccg gcagcggccc
     2821 ccattgctgc ggtcgttgcg gcacaaagcg cagacaaacg cggtaactgg ttatggatgt
     2881 aaatcgcgct caaatgcgaa agcatcagcg cacgcgccag ccgttcatca tccgctccga
     2941 agtgttctgc taccaccacc acaggcattg ttgcggtgat cccctggtta cccgagccgg
     3001 agttactcat agccggaagc gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac
     3061 gaatcacaat ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca
     3121 gcgtcgcgcc aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat
     3181 ttagcttcgc ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact
     3241 tcaggatctc agccagcgtc gttctggaaa gcaccgtcag cggagactct tgctcgccct
     3301 ctgccacaca cgcctgctgg gtaaacacca caccatcgtg cgtctcaata tgcacaatgt
     3361 tggtatgccc gccgacgata gtgacacacg cccacttctc accgttccag actttggcgc
     3421 gtgagaagag gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca
     3481 gcagtgcttt ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccag
     3541 cgttggcatt tccacctaac gctcccagcg ccgccgcaat cggcagcccc accattcccg
     3601 tgccgggaac ggtgacgccc agaccgtttt tcatcagatt tggcgaaacc caggcttcta
     3661 cacgttcaac cggaccttcc agttctgccg cagcaaccgc cgccgccaac gccagtgaaa
     3721 tcggttcagt acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct
     3781 gccataacgg atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc
     3841 aagaaaatgc caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca
     3901 gagacatccc tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca
     3961 ggcaccctac catgccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt
     4021 tcagtacgat ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacga
     4081 gattttcgtt aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt
     4141 gcgttgcttc gcgaaagcct aaatagacgc caaagaatgc agtcatgaca gcaaagatat
     4201 tgagaatgac gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg
     4261 caatcgccag cgcggaaata ttctgctcat aggcttttac cgcttcgtca tgtcccatcg
     4321 ccagcgtgaa cgacacggcg tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat
     4381 tcattgcccg caatgcttta tgccgcgcta cttcaatcga tttttcccgc gagcgataag
     4441 agatcaccat cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg
     4501 taataatggc gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat
     4561 gccacattcc caccatcgac acgcccagcg ccgcgaccac cagcagcttg gtcagcacca
     4621 tgccggtcga aattttgaat aacaatttct cgccgcgtga ggagatcgcc accagaatgc
     4681 aaatcagtac cagaccataa aagggactgt ctgacagcaa cccttccgtc acgccgaagg
     4741 tatgcaggta ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca
     4801 ccagcattac gaaatagagc gcacctaaca ggatgcccca gtttttacct aaataaccgc
     4861 taatgacact cgggtaatct ttacattctg gtgattctgc cagcgtatta ataaacaacc
     4921 gctgaaacag atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc
     4981 ccatcaaacc gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac
     5041 tcataatcac ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct
     5101 cactcattcc agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg
     5161 caatttccat aatttctcgc tcaatatttt gtagggctta ttattttttt ccgagccgca
     5221 tcaaggcgat atgcggtcta cgtttttgca ggcgagttat tagaataaag aaatgatacg
     5281 cgcgaggtca gagaaaatct tcacaatcaa gactttgaaa taacggagat ggataagaat
     5341 tttctactaa attaatcgca agagaatatt cattctctat ttgcggcaga tcacaaaaaa
     5401 aaggtgcaca tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact
     5461 ttaatggcca gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt
     5521 tcatacatcg tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc
     5581 gccatccgcg cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg
     5641 atttgtacct gtccggcaac cggatcgcag gtcagcccaa ggttatgctc catcgcgatt
     5701 tccgccgcat tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgcc
     5761 gccattgaac aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag
     5821 gcgttcattt tatacagcgc gccaatagcc cccgctgcca gaaaatagcg ggcaattgac
     5881 cgctcgttta ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg
     5941 cacgcaccgt tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc
     6001 gccagcgcgt acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg
     6061 ctagaaacca gctgacgacg cagcgctacg gcacgacgcg gcacattgag cggaccaggc
     6121 agcacgcctt cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg
     6181 gcaaaccctg cgtcaatttc cgctttactg cgcagcgcta gctcgttgtg catcatcagg
     6241 ccagatatag acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa
     6301 tcgtaaggta cggacgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata
     6361 aacccgccgc cgacagagta ataggtttta cttaatagcg cttcctgtcc cttccaggca
     6421 gtgatccgca ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt
     6481 gctacaggaa aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt
     6541 actaactcta taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc
     6601 agtcccatga taatggcgac atccgtggca tggcctttgc ccgtcagtga caacgaccca
     6661 tacagatcga ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca
     6721 ataaaacttt ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca
     6781 attttgaaaa tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag
     6841 tggagcggaa cgaccttgcg accgtcccgc tcacgaggct ttacgcacta cgtactgcga
     6901 tagcttcaat ttccagcttc acatctttcg gcaaacgcgc aacctgcaca cagctccggg
     6961 tcgggtaggt cgcctgatgc tcatcgaaga actgcttata gacttcattg atggtggcaa
     7021 aatcattcag atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg
     7081 cagcaaccac gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag
     7141 ccgggatctc accggtctgc gggcaaaccg gaatttgccc ggaggtgaag accatgctcc
     7201 ctaaatcaac gccctgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa
     7261 tctttttcat acatcctccg gcgtcagagc gcctgggtaa aagtacgtga aataacatcc
     7321 tgttgctgtt cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc
     7381 aggttaggat atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg
     7441 ttgacgttga ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc
     7501 aggccgacaa ggttggtttt acgtactgga tcttctttgc ccagcgccgc aggaacgatt
     7561 gagaaggtgt acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc
     7621 aatgaggcca cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac
     7681 ggtgttccgg cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta
     7741 gaagtgatag tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta
     7801 attttcttca taaagcgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg
     7861 ttgccgtact gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca
     7921 cggattggtt tcacgcgggc atatttgatg gcagacaggg agtccgtcgc caccgacagg
     7981 cccgcgatgc cgcatgccat agtgcgatag acatcacgat cgtgcagcgc catcagcgaa
     8041 gcttcgtagc tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc
     8101 accgccagcc agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcacg
     8161 tcgtccatca gcggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg
     8221 ccgttaattg cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt
     8281 tgcttaccaa tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag
     8341 tcagtacgca tcagatcgtc attttcatac tgcaaggaag aggtgacgat cgacacctgc
     8401 gcggcatatt ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt
     8461 tccggtgccg gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc
     8521 agcgtacgac cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg
     8581 gagaatagcg aatcaaattc cggtgtacgc aggaagcgta ccatacggat cttcatgatg
     8641 aagtgatcga tcagttcctg tgcctgctgc tcattgagta cgccagcttt aaagtcgcgc
     8701 tcaatgtaga tatcgaggaa cgatgccgtg cggcccagcg acatcgcgcc gccattttgc
     8761 gatttcactg ccgccagata agcgaagtag agccactgca ccgcttcctg cgcattctgc
     8821 gccgggcgag agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca
     8881 tgacgatgct ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt
     8941 tccagacgag actggagatc ggcaaattgc agttcgcgtt cacgtaccag ataactgatg
     9001 ccatacagcg ctacgcggcg atagtcaccg ataatgcgcc cacggccata gccatctggt
     9061 aaaccggtca gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat
     9121 acgccctggt tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt
     9181 tctcggccat aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc
     9241 gcacgtttca acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata
     9301 tatcccgcat catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg
     9361 tgggttgcgt tttcgatacg gataccttcc attacttttt cccacaattc cgtggtggca
     9421 ggcgtcgctt cggcgaggaa agattcatcg ccttcatacg gtgtatagtt atgttgaata
     9481 aaatcgcgga cattaatttc gtttttccag tccgtacctt taaagccaag ccatgcgtcg
     9541 gcgtacagct tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata
     9601 cttcaactaa attatgcaaa ttctgcgggc gcgttaactt tgcctaaatg aatggcatcc
     9661 aaagcaatca ttttttcttc gttagtcgga ataacggcac aaatgacacg cgcattttca
     9721 ctggaaacaa ttcgctctcc aaaagagtta gatcgattat tcatttctgt atcaatcact
     9781 acgcctaata cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct
     9841 attccgccgg tgaatataat tccatccagg cgacgtaatg aagctgcgtg tccggcaata
     9901 tgacgggcaa ttcggtgaac aaaggtttta attgccagtt gcgcgcgttc gtgaccttca
     9961 tgccaggctt tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc
    10021 gactctttat tcactacgcg ttccaggtca cccagactct ggttggtttg gctggcgacc
    10081 caggacatcg caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc
    10141 ggcgtcattc ccattgaagt atcaacactc tgaccgttgc gaaccgcgca gattgacgcg
    10201 ccattgccaa gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcagcgaa
    10261 tgtgcgcgct gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct
    10321 aactcttcat aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg
    10381 tggaaactgg tatcaaatac cgccacctga gttacgcccg gaaataattg ctgcgccgat
    10441 tcaataccac ttaaattggc gtaattatgc aggggtgcca gtggagaaac gcgacggata
    10501 ttatcaatga cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg
    10561 atgcggtggc caattaaggc cacactgtca tttaaattcc gtttttccag ttcaaatgca
    10621 attgccttca atgcaccttc gtagctgtgg tgagccagcg gtgctggctc tcccccattt
    10681 acggataaga atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca
    10741 cagtcgctgg catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa
    10801 acaaccggaa attcattcat ctcttttctc atcctgagtt acggattaaa acagtttgta
    10861 tacgatgttc aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc
    10921 acggtatttc gccagagacg gcgctttacg gatggcatac atcggcaaca ggcacagcag
    10981 ggatgcgata atcggtgcgc ccatggcttc aataaggtcg aggatgttcg ggttggcgta
    11041 ggcaacaacc caggtggagc ccatgatgaa gatcatgctg atagtgttca gtttacccag
    11101 cgacacttta gttttgtcgc ctttataacc gaacttcaga atcagaccat tcaagccttc
    11161 cagcgtcccc agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga
    11221 agccgcatat tccagtgtaa tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg
    11281 gttagccaga taagaaagca ctggaatatt ctgcgctttg gcttccgcca tgttggccgg
    11341 agacagcgta aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct
    11401 ggcacgagaa atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc
    11461 atactcttca cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa
    11521 aaccatgatg gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga
    11581 cagcgaaccg aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacag
    11641 agaaatcagc accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat
    11701 cagatcctta ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa
    11761 gccgcgattc agcggtgcaa atccgagctg gttttcccag aacgtcataa aggtattggt
    11821 aatagtaacg ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat
    11881 aaccacgccg ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg
    11941 gttagagcca gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta
    12001 tgccaatacc aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa
    12061 gaacagcacc ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga
    12121 tttacgccag gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc
    12181 ctatcctcaa cgaattaatt aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag
    12241 atcgatattg ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa
    12301 tttaccgctt aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt
    12361 gcgctgaatt aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc
    12421 atcgactaat tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc
    12481 atccgccagg gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc
    12541 catgccgtga acgttttcag actgtacgcc aataacacga atggtcgggt taatagattt
    12601 aattgccacc gcaataccag caattaaacc gccaccacca attggcacaa tcacgttatc
    12661 gacatcatag agatcttcca taatttccag accaatcgtt ccctggccag caatcacttt
    12721 cggatcatcg taaggtggga taaaaatacg gccttccatt tcgacaattt cgctcacttt
    12781 agcgatagtg tcgttgaaat tatcaccatg cagaacgact tctgcggagt agtcgcacgt
    12841 tgccgctact ttggattttg gcgcaccttt tggcatcacc actttaccgt cgatacccag
    12901 catcgcgcag gagagcgaga ccccttgcgc atggttgccc gcagaacagg ccactacacc
    12961 tttacgtttt tccgcatcag tcagtgaact taatttatta aatgcgccac gaattttaaa
    13021 tgaacccgta cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact
    13081 aaaatagttt gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc
    13141 ttcaataatg tcatcaatag caaccagcag atcgtatgta atatgcatta taaaacctct
    13201 tcgcctgaat taaatatgta ggtaaccgta accgacacct gcaaaacaga caggtggatt
    13261 atttatgttt aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac
    13321 ccattataag atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata
    13381 cgataatttt tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga
    13441 atagtaataa attgatttga accaaaaggt gacgtcatat cacaaggaat tacagttaag
    13501 aaatcagcat tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt
    13561 tcaatactga tgccatttct ttgtaacgta gtaagaagct cgctgtagta ccccatattg
    13621 gtttgtggca acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat
    13681 gttcgggact tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa
    13741 tcctgaagct tcatttctgc acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg
    13801 attgccggta agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac
    13861 acctctttga acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa
    13921 ccaaatgaga cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt
    13981 ttcatttcac gggtaatgga ttcggaacgg gagagtaaca attgaccggc aggtgttaat
    14041 gttacaccgg tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta
    14101 atgattttac tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagag
    14161 ccacttctaa tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg
    14221 ctcataacga cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaatga
    14281 acaggatatg tgcgaccact cacaaattaa ctttcaatac tttccatggt atcgttacgc
    14341 aaattaaatg cctttaaata tcaacaagtt aaagtataaa aatcagcata aaccctgatt
    14401 tttgtcaaaa aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt
    14461 ggttaatata aaaaaggggc tgagaccata tttcaagcaa ttaccggata attaccaggc
    14521 aaaatttgag ttaaatttaa tgagcaagtt ctcaaatttt attataaata aaccattttc
    14581 agcgataaat actgcggcac gtcacatttt ttcacgcta
//
LOCUS       DAMZII010000115        14536 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZII010000115 DAMZII010000000
VERSION     DAMZII010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.115"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(88..435)
                     /gene="trbA"
                     /locus_tag="POA77_003571"
     CDS             complement(88..435)
                     /gene="trbA"
                     /locus_tag="POA77_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDI6366939.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(451..1194)
                     /gene="traF"
                     /locus_tag="POA77_003572"
     CDS             complement(451..1194)
                     /gene="traF"
                     /locus_tag="POA77_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDI6366940.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     IRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1187..1444)
                     /gene="trbE"
                     /locus_tag="POA77_003573"
     CDS             complement(1187..1444)
                     /gene="trbE"
                     /locus_tag="POA77_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDI6366941.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(1471..3279)
                     /gene="traN"
                     /locus_tag="POA77_003574"
     CDS             complement(1471..3279)
                     /gene="traN"
                     /locus_tag="POA77_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDI6366942.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRNVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGGDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3276..3914)
                     /gene="trbC"
                     /locus_tag="POA77_003575"
     CDS             complement(3276..3914)
                     /gene="trbC"
                     /locus_tag="POA77_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDI6366943.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3923..4915)
                     /locus_tag="POA77_003576"
     CDS             complement(3923..4915)
                     /locus_tag="POA77_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDI6366944.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPALPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4912..5544)
                     /gene="traW"
                     /locus_tag="POA77_003577"
     CDS             complement(4912..5544)
                     /gene="traW"
                     /locus_tag="POA77_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDI6366945.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMRRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5541..5927)
                     /gene="trbI"
                     /locus_tag="POA77_003578"
     CDS             complement(5541..5927)
                     /gene="trbI"
                     /locus_tag="POA77_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDI6366946.1"
                     /translation="MSSTQKPADVMAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDRASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(5924..8551)
                     /gene="traC"
                     /locus_tag="POA77_003579"
     CDS             complement(5924..8551)
                     /gene="traC"
                     /locus_tag="POA77_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDI6366947.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8711..8932)
                     /gene="traR"
                     /locus_tag="POA77_003580"
     CDS             complement(8711..8932)
                     /gene="traR"
                     /locus_tag="POA77_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDI6366948.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(9067..9582)
                     /gene="traV"
                     /locus_tag="POA77_003581"
     CDS             complement(9067..9582)
                     /gene="traV"
                     /locus_tag="POA77_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDI6366949.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9579..9830)
                     /gene="trbG"
                     /locus_tag="POA77_003582"
     CDS             complement(9579..9830)
                     /gene="trbG"
                     /locus_tag="POA77_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDI6366950.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(9842..10072)
                     /locus_tag="POA77_003583"
     CDS             complement(9842..10072)
                     /locus_tag="POA77_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDI6366951.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(10026..10616)
                     /locus_tag="POA77_003584"
     CDS             complement(10026..10616)
                     /locus_tag="POA77_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDI6366952.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(10606..12033)
                     /locus_tag="POA77_003585"
     CDS             complement(10606..12033)
                     /locus_tag="POA77_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDI6366953.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(12033..12737)
                     /gene="traK"
                     /locus_tag="POA77_003586"
     CDS             complement(12033..12737)
                     /gene="traK"
                     /locus_tag="POA77_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDI6366954.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(12748..13314)
                     /gene="traE"
                     /locus_tag="POA77_003587"
     CDS             complement(12748..13314)
                     /gene="traE"
                     /locus_tag="POA77_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDI6366955.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13336..13647)
                     /gene="traL"
                     /locus_tag="POA77_003588"
     CDS             complement(13336..13647)
                     /gene="traL"
                     /locus_tag="POA77_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDI6366956.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(13662..14027)
                     /gene="traA"
                     /locus_tag="POA77_003589"
     CDS             complement(13662..14027)
                     /gene="traA"
                     /locus_tag="POA77_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDI6366957.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(14060..14455)
                     /gene="traY"
                     /locus_tag="POA77_003590"
     CDS             complement(14060..14455)
                     /gene="traY"
                     /locus_tag="POA77_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDI6366958.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
BASE COUNT         3350 a         4087 c         3489 g         3610 t
ORIGIN      
        1 gccactggat tgcggtatgg tttatcatat ggtcttcagt aatcaacctc attattttgt
       61 atggttttta tgaccgtttt atatgactta gcctgtcata agccccctta taaaaaacca
      121 catcactccg gaccataccg atgacaacac aatacagata aatattcttc tgaacctgtc
      181 gaagctcgta acgtccggta atgcggccgc aactattgaa acaaccagtg ctgcgccctg
      241 actcataatt gcaccacaca gaaaccagag actggcggta agcacatata aacacgcgat
      301 attaataaca aggaaaacca gtgagctcag gagaaacatt ttcctgtcat tacgctcaga
      361 aaagaaataa ccggcaataa tgacaaatat taacagaaca acacctgtaa acattttcaa
      421 ataatcttca ctcataaatc aaatccgcga ttaaaaattg ggtttaaaat cttcagaaac
      481 gttcaggaac tgttttgcca ggtcgtcctg cgaaataaag ccgtatgata acgggcgaat
      541 actgccctgt ttcgggtcaa ccagcatcat ggccgggaaa tatttcacgc cgaggcgctg
      601 cgcctgcccc tggtcagtcc gggaatccgg caacagcgga ttaatcacgc catccacgga
      661 aacgggaata acactcagac catacgtatc ccggaagcca ttaatgacct gcgccagttg
      721 cccgtcgatg gggtcctgcc cccggtaaaa aaacatgatg ccgtagtgtt cagccagttt
      781 cgcaatggcc tgtcgctgct gcgcctggtc tgctgccagc tggttccgta ccgtgccgtt
      841 gtaatggctg tactgcaggt tatagtccag ttcaggatgt gccagcatgg cctttctggc
      901 gctcatggtg aaaagcccgg cctgctgagt ccagtaattt tgcagccgga aatatttcac
      961 aaaattatcc acgccgggat acagaatggc ttcgtacagc gcccgctttg ttgccgtctg
     1021 cagtgcggcc agtttctgca taatatccgg ttcctgacgg ggggctgcag gtacaggttt
     1081 attttctttt tccttcggat ttattttctc gttataccac tgccagcctg catcttttcc
     1141 tgacgccgga aaaataaagc agcagagtaa cagtggcagt aatgctttat tcatcttcat
     1201 tcctttcatc actttttctt tcatcaggtt tctgcaggtc agtcagggca gaaaaacaaa
     1261 aatacagaat aaacaggagc acaccggagt aaaggataac gaaagcagcg gcaagcatcc
     1321 cgtctgccgt ggtttctttt accgtatccc acgaccagat aattaccgga gaaatgacaa
     1381 tcgctgtaat caaaagccgg ataagaaaat caataaaacg gttcgacgtg aatatgactt
     1441 tcatcatttc ccccggttat ttcccatacc ttactgtcct gcctgtttca gctggtcagc
     1501 aatctgctct ttcactttct gcgtcagaac gccactgtcc gggattttct ggttattcat
     1561 caggtcttcg tagaagttag tgaaatccag tctgttgaac tgaattttct gcagctcatc
     1621 aaccgtaatc ccccgacagt caggatgctt cgcactacca aaactgatac gcaactgacc
     1681 gttgcgcccc tgctgctgga ctatctgcgc cagcttcgaa tcaaactgac agtagctgcg
     1741 ttttttctcc agacagacac ccagcacttt cttcgaacag aactccccga cgctgacggt
     1801 cagtttgttt gattttgctt tagccagggc cttttcatca ctgctgcatt tggccagccc
     1861 gatatcctgt ccccagccgc tgtccttaca gcagttgctg tatccggctg cagccttctt
     1921 acaaaatttc gcctgaccgg tgaaagcacg gacatccccc ccgttcagcg ccgcaacatc
     1981 cttacctgct gcggccagtg ccgccagctg agaaacggct tcggcaaaat cattactctg
     2041 cccgctctga gccttatcgc attcgccatc gagacagaat acgtctcccc cacagaccat
     2101 cactttcccg gatgtttttg actcacagga gtacgtggca tattcatgca gacaggcacc
     2161 ttcttcggag taaaaggcac actggtgtga caccagggta caggcaggat tgtcggtgta
     2221 ggtctgacag gttcccttgt ctgctgactg cgtgacatac gtgtcccggt atgcccagca
     2281 gctctggtac atgctgtatg actgaccgtc tttcaccagc gtcctgttcc cgccagcctc
     2341 cgtacattct gtttttgtca gtttccccag ctccttgctg aacgggcagc tttcagacca
     2401 gacaacatac ggggtgtacg aggctttttt taccctgaca accagacgca gcttcattgc
     2461 cgtatgacgt ttgaattttg tattcccgac ccggacgggg tgagatgacg taaaagccac
     2521 accggcagtc agtgttttct gtactggtgt aaatgaggca tctgctgagt atttggcatt
     2581 ccagctcagt ggggttccca gtactgtaat tgtcataggt acactcttct gcaagtaagt
     2641 acgactccag ctgtaatgcg catcgacaat ttcaccggta acgggagagg taatggagac
     2701 aacatactga ccattcactt cacgtgcagg tagctgactc atttcatact ccagcgtccg
     2761 ggtttcccat gtggtactcc cctgcagctc catacgggca gttcgtgtgc agtactgctc
     2821 cacctgtaaa tcccgctcac aggtgtaatt cgtgtattca ctcctgctaa tctcctgcgc
     2881 actgcactgc tgcccggtgt ttcccacaat gctgtcagcc cggttcacca cattcctgcc
     2941 ggtctggata aacggtgcat ccggtgaaag aatgtctttc ggcttgttca tgaatgactc
     3001 tgtaatggtt tttccggttt caccggtcgc ccattccgtg gtgccgtcat ttttcaggcc
     3061 gccatccccg ccggcagtca cgccaccgta atattttgtc tcgtccggat tcgcgttata
     3121 gccggggata ctctcctgtg gcttaaagcc ctgaatactg ctgcttccct gccctttgat
     3181 ctggtgagca aaatcagaac cggcccggta atcactgttg ctgtcagcct gtgccatgcc
     3241 ggcaaccaga gccagtatca gaggtaaaat acgtttcatt tcccggaatc tcctttccct
     3301 gccagtaaat catgagccac ctgccggcag tcacccgtgg cggccacttt ctccagcgcc
     3361 tggccgaccc gcaggtttcc ccggatgatg tcgtatccct gactgcagaa caccaccagt
     3421 gccggtacag tgcgaatgcc gtactgtgaa aacagcgtcg ggtcgatctg aacaccatca
     3481 gtcgcgccgt ctttcaccag ggacaacaca gcttcagcgg tggtcttcag gtcattgttc
     3541 accatgcccc gcagtgtggc cggaataccg tagtgccggg tttcgcccag cattcgtttc
     3601 agcccctctt cgggaatgga aaacgacaca aaatacagag caccctgccg gggtttcccg
     3661 tcctgcgaag cctgctgttt acgaaccagc tcatccagga aatggttatc tgaacgctga
     3721 agggggtttt ccagcacctg tttctccgcc caggctttca gctgatggtc aggtttttca
     3781 cgcagttgtc tgcttaaatt ttcctgctgc ttcaggaact ggcggttttc aggagtgttc
     3841 acgttttctg acgccataac cgccccgttc agcatcatca gcagtgctgc cagagatttc
     3901 atactcagct tcatcattca cctcacagga agacacagtt acgtttacgc cacatcaggt
     3961 agccgaagtt tttctttgtg ttgggcggat ttttcccggt ttcccagcgg gtcacgctgc
     4021 gcccgaacgg gtggcactgc ccgctgtccg gatacatatt caccatctgg taacgccagc
     4081 gttctttggg caggattggg gacggatatt cattacagac ggcgttattt tttccgatgg
     4141 tttccataat catgccctga cggtgcagct tgaacgccat gcgttcactg accagcaggg
     4201 aggactgcaa cggactggac tcattactca cccagccatt gaacgggtac atacttccct
     4261 gcgaaccggc acaccagaac agaacatcga gaggcatatt aaaggcgctg gcaatcgcat
     4321 ctgctgcgca ggctccctgt gctatcggat tggcaaagat gacagcttcc ggattgagaa
     4381 tggtggtcag gctgctgtcc gtccaggtgg ggtcgatttc agaaagataa gcgatatcca
     4441 ggtcaccacc ttccagacag cccagcgatg tgatgatgtt cagccagtac gtcagcgggt
     4501 atttgtacca gtgaacgtga tagaacgccc catttacctg cttctcatcc tttttcgccg
     4561 ttccctgtgc cgttttacca aaagccggca ggctgaagcc caggttcacc atgcatcccg
     4621 gtgaccgggt gacgtccgtc aacgccatcg gctcccagta accaatggcc agcccgatac
     4681 gcctgaacag cggcggcagt gccggacaaa tctgaatggg catcgacggg ttcgccgtgt
     4741 cggggacctt cccctgactg actttgatac tgcccagcga gagcgggaaa atacagctcc
     4801 agcagatatc tgtgatcggg tttacaaaac gcccctcaca ggcagaatcc gcagacgccg
     4861 cagggacatg accggcgaaa aggaataaca tcagcagcca cagccttcgc ttcattttct
     4921 gccctcctct gccggaataa attccacctt caggaagcga tcgccgggaa cggcgctcac
     4981 ccgtgccggg acctgatcaa tgccgaggcg ctggcagagc acaccgttct ggtcaaagta
     5041 aacacggctg tccagagact tctgcatctc cgggatactg ccctgcacga ggataatttt
     5101 gctctccagt gtgggcggcg tctggcgtct catccaggcc acctgcgccg gatcatcgcc
     5161 attgatgaaa tacagcgtct ggttaaaagg aacatactgc agcggattca tcacttcacc
     5221 ctgacgggca aacacccgcc cttcgttatc ccggatatcc gcagccagtc tgacggacgg
     5281 gtcaaacaac cggctgccgt atttctccgt gcgtccgata ccgggcacgg cagggggccg
     5341 cagactgttg cggatcaccc gctccttaaa ggcatccatt ttccggccca tctcaccgga
     5401 ctgctccagt gccgtcaggc gctgcatgat caccgtcagc atgtccggct cctttaccgg
     5461 ccacagatcg ccccaggtac caagatcggc ggcggccaca ctctgccccc atatcagcag
     5521 ggcaatcagc ccccggcatc tcatggttcc gccctcattc gccgggcaat atcctgctgg
     5581 atttcccggg tgatatccgg tgccccctgt accacagcag gcgacaccag aatgaccgcg
     5641 tggtgtttct gctgccacgc ctgcaggctg gcttccagtg ccgtgttaaa acgcgctgaa
     5701 agggcttttg actgcgcttc tgacagttgt ttctgactgg ctctgtcaaa aaacgcatcc
     5761 acggtctgtt tcatgttgaa ggcgacagtc accggtgcgt tcagtctgac aatgccgtaa
     5821 ctgacggctg cgttcagtaa caccattgcc aggcatcccg gcaccgtcca ccaccagtgg
     5881 ctgcgtcgtt ctgccatgac gtcggcgggt ttctgcgttg aactcatgcc acactcctgt
     5941 atttctcatg ttcttccagc caggcttcca gcgaagccat ttccggccct gacttcttcc
     6001 acgccagctg ccacactgcc tcatgaatac tcagcccctc tttacgcttc tgctgcacaa
     6061 actcaaaatc cgggccgtca gagctgtaca tggcgcggct taacgggtcc acaaacagac
     6121 ggtgccagga ggaatggttt tccacctgca gcaggaagga actgaaccac tggtctttgg
     6181 ccgcaccaaa cttaccaatc atgtcgcgct gcagtggcag gaactggtcc ggatacagtt
     6241 ggttgtattt cgcgaactcc ttcgcactct gtttgaggat aattttgtag gaggagttac
     6301 cccatgccgc gcgggcagca ctggaggcct tatcagagtc aaagtcgacg atgttctgtg
     6361 tgatggtgat ataggcaccg gtatgacggc gggccgtacg gtagcctttc tcaataaatt
     6421 caccgacctt gtgatttttg aagtccagca gacgccagcc ttcatcaata acgttcagtt
     6481 tcttgaggtt acgcggcgtg cggtacatcc tgttctcgat gtagataatc agggagaaca
     6541 tcaccgcaac cagcagtgac ggacggtctt ccagtccgcc cagctccagc accaccattt
     6601 ttgcgtcatc ccgcagggac ggctcatcag agttaaaata ctggccgtaa gtgccgttgg
     6661 cagtgtactg gtcaagcagc acaatcattt cgtccagacg gctgcggata gtcggtgact
     6721 cggcatactg ctcgctgtca ctggcgtttt tcaggaaatc caccacgtca tcaatacgtg
     6781 ctctgttctc tttggccagc caggaggctc tgaccgcctg caacagcaga ccttcatgca
     6841 cttcatccag gttaccgttg gggctggcca tcactgacaa ctggtcacgg acacgctccg
     6901 ctgactggtc aatatcggtg atgttcgcaa acgggttaaa acgcagggtt tcaccgtcca
     6961 gatacacccc gcccatgttc tcacacaggg acttgtatcc atcccccatg tcgaacacca
     7021 cggcaaagcc tccggagtcc agcacgctgc ggataagtgg ctgtatcagc ccggttttac
     7081 cggccccgga ggtgccacag accgccatgt tgtagttggt gttattcatc ccccggaaga
     7141 aaatatcgat aaacgccagc tggttacggt aggtgggtgc cagcagacct gccggtgtca
     7201 gggggttatc cgccactaac ggcatcaggt tggcaacatt aaagctctct gcgcgctgaa
     7261 ccactccggc ctctttcaac tgtttaaaca gccctttccc ggccataaag ggcaggcagg
     7321 tcaggaaatt gcgcatgtgg ttaaagcgcg gtgaaatcag ctcaaaaccg tttttacgaa
     7381 agctgttcag gatgtcctgc tccacttcca gcgccgtttc attattgtcc ttgcagaatg
     7441 ctgtgatgtt gaggaagtag gacaccacgg aggactgacc ggagcccagc cgctgacgca
     7501 gttcccccca ctccttcgcc tctttctcca cggacggaaa ccatttggca taggaggtct
     7561 tcgacttttt ctccaggtcc atgtacttca ggttggcttc gctgtgggtt ttcacctggt
     7621 cttccaccac cagagtcagc gtcaggatga acggacagga gatggacagt tccgggttca
     7681 gcaggttgct gtagttgtcg gccacgttcc acaggaaggc gatttccggg ttacgggcca
     7741 gatggaaatt caggatgcgg gccgtgctgt tcctgccgtt ctcacgcagg cccagcgtca
     7801 ggtaatcagc ccggactttc agatcaaaac tgtcctccac acactgataa ttcagatcag
     7861 aatacgggtc cagctgacgt cttttcgggt acagggagtc cgggttatgg ttaatcatct
     7921 ccccgacaat atcgataaag gcctgtgcat ccaccgtctg tgtggtgata ctggccccct
     7981 gtaacgacgc ccggatgatt ttcaccaggt tttccatttc cagaatgtcg gcccggcttt
     8041 ttttcttcga aggagaacag tacgagataa acacccggta atggcgcagg gtcaggggca
     8101 gattcatccc ctccggcagc ggaaactgtg tcgctgccgc tttcatataa taggcccggg
     8161 taatggcgtt aaaccgttca gcctgttcac ctgaccagga gaactcacgc agcccgtatt
     8221 caatcctgtc accaaccagc tgactggaca tcagatggat acacaacgga ataccgcgcg
     8281 gcagtttggt gcgcagcatg tgatcgaggg cctccacaat ggactcattc gccccattga
     8341 tggggatggc ttccagcata aagcccatcg tggtgtcatt catgaacaga ccggattcct
     8401 ggttgtaatc acggtacggc agcagacggc tgaactgcgg gaagctcatt tcgcccagaa
     8461 cttcattggc ctttgcggat tcgtcaggca gtttcagtgc tgtgacgagg gagttaaccg
     8521 cctgagtgac ggcctcaagt gggttattca catgaattcc ttagttgaaa aataaagcga
     8581 gaaatgcggt agccatgaag ggaatacctg taattaacgc gccatttccc ggcgcatata
     8641 caaagatttc tgttttctga atatccactc atgttcatcc ggaattaaat cactatgttc
     8701 tgcgactgac ttatgcataa tgttttctct gtctttcctg atacgcctga cattcaacgc
     8761 acaacgtcac accgggaaat attttccgcc gggcttccgg aataggattt ccgcatgctt
     8821 cacagagata aacaggaata ccatgagcat ttattttctg gcgtatccgg tttattcctg
     8881 tcatggtcag ttgttctgtc actgaatatg cttcatcggc ttcatcactc acggaatatt
     8941 cctcccgaca tgttctgaca cgctaccgcc catccctttt cacgcacagg gcactcgttt
     9001 tacaggcaga ttttgaatac agaaagcccg ccggcattgc cggctgatat acaacttcag
     9061 ggccagttaa ttaatacgtg gttttcccca cgcagacggt ttaataacaa aaaacacgct
     9121 ggatggctga tgataaacat cctggttatc aatataaggc gctatccata atgctgccgt
     9181 ctgctcaccc gttcttagcg gacgaggagg tgttaccggc gtaactgaac tgaccggaac
     9241 aaccgttttc acttcccggg cagcggtaaa cagcgggcga ggtgccagca gtttctgctc
     9301 cggatgtgct gtcaccgcag gtctgctgcc ggacggagtg gtcgcggtga cggtctgcac
     9361 cggcattgtc cggaagttcc cttcagccag gcgcggcagt gatgccgcaa ccggctttgc
     9421 ttctgaggac tgctccagtt ttttggcctt ctcattggcc tgctccatcg tcatacaggt
     9481 atcggatgtg gtggcgttac actcaaattc cgtactggtt ccggcacagc cggaaagtaa
     9541 taaggtcccc agcagaggaa taaagaaaga aatctgtttc attgtccctc acctttctca
     9601 tcgcagttca caaaatcctt cttcaaatcc ctgaccgtac aggtttttac aatgtcagga
     9661 ttttcaaaaa ccgtactccg tgatgattta taaacatatg ccacaatgcg tttcccaccc
     9721 gcatcaggtt ctggtgcact gacttcacat aacggaggtg ttattccgga ctcatacagc
     9781 accggataat ttattttttt gacgctgcca tcagaaacca gcttattcat cgaagggttc
     9841 cttatatttc tgaattcttc atcacaccga cagcagtcat attaaaattg tccggcaatg
     9901 actcagaacc gaatttctcc ggtgcaggaa gtacgattct tgtggcgcag ttactgagaa
     9961 cagcatgcat aaaatcatct gacacggttt tacccggcac agagagggct gtaataacat
    10021 taatattacg catgttcatt tttatctccg ttgtttctgt ttgttctgtc atcaggaaga
    10081 taaacagggc atttcctgat ttttcccggc agggaagcgt cttccgtata aacttccttc
    10141 agatgttcat ctgcggttac cacccggata catttcattt ttccgggtcg caccggatat
    10201 ttcccggtga tcgctgccag cgagacatac atcagtgcca gtgttgccca taatgcggtg
    10261 accagttgca tcagtgaccg gtcagtctcc ctgacataat ctgcagcatc agccgcgcgg
    10321 taatggcaga tatcctgtat ttctgatctg cctgaagaat cagaggaaga aaaccagcat
    10381 tcactgaccg ggaactgtcc ggcaggtgct gtttgtctta cgaagttaat ctccttaacc
    10441 agtaatttcc ccggcgtggt gttgtccttc cataaaacaa acagagccat cagggccatt
    10501 gtggccagtg gcagaatgac ggtgtacttc agacaccaga acagtcctcg cagaacccgg
    10561 gcgacaacat accgggcggc atggcacgcc tgacgggacg acatattatt tgccatcgtt
    10621 gccccctccc tggctgccgg acataaactg ctgcggattc aggttgccca gctgcttcag
    10681 catctggtca gtattaaagc cgttaagatg actttcagcc gatggcggaa caggaaccgg
    10741 actttccgga ttgtcttctt ccgctctgct ctgcgtgcgt tccagcgcca tctcttccac
    10801 ggttttcagc tggaagccgt cctggaacac cacggtcact tcgttgcccg caccaatcgg
    10861 tatcaccggg tgatactgtt cggcacgttt gatgtagtag tcactgagcg tctgtgcggc
    10921 tttcgatgcg ccgccaccga tacccatttt caggacatca ccagccccgt aagcggctgt
    10981 ggcacccagc ccgacagccg gctgggaggc gcgctccatt ccctgaccga taccgtcaac
    11041 aaatcccgcg ccccatgccc agccgaggat tttgccgtta cgcatcacca cttcgccctt
    11101 gataccgttt ttaccccgga agctgacatg ccccttaatc ggcatatcaa tagttttgcc
    11161 gtccttcagg caactgatat tgcgggtgcg tacaatggca cgctcactgg acacatcccc
    11221 ccaggcttcc agacccacaa aacatcccgt tgcgtcatac gtcttgctgt tcggcatttc
    11281 caccaggccg gtgatacgca gctgcatcgg caccgtggat tcattaccgg tgacagaggc
    11341 attggcatcc gccccttcaa tcagcatggc tttcgcaaaa cttcctgacg gaatgtacgg
    11401 cagtgatggt ccctgttttc cctcattacg ggtaaacacc ttacgctgta tccggttagg
    11461 caccggcaca gactggtacg tcacctgtgg tggtggcgtg acaccattcc cgggataaaa
    11521 cgccgtcggc ggtggaacag caacgctgcc ctgcggcggg aaggatacgg gggtgtttcc
    11581 tggctgtggt tcgccttccg ggccgggcgg tgaggcaggc atctgcggta caggctcacc
    11641 ggtcgccgtg acgggattag cacccagggc ttttacctgc tctgccaggg cggcattgtc
    11701 ctgtcccaac ttttcaatac gacgctggtc atcaccgcgc tgtttgttca gaacatccag
    11761 ctcacgacgg atttcctcat actgcttctg catctgcgct gccgtcacct gcatctcggt
    11821 ggtggcatgc tgacgcacct tgtcatcaaa ggtcgtatcc accacaccgg tcatatccgg
    11881 cacaggctcc tgttcagcca cggtttcacc gttaccggac atgtccacat cagacagata
    11941 cagtgcccca ccaatcgcgg aggctgtacc gacaaccaca atccccagcc acaggtactg
    12001 cttgcgtttc acaatggtat tgatactggc catcagttgc cctccccgtc acggatgaca
    12061 tacacatcca tgcgcgcacc tgccagtaac tggctggcag gctggctgaa catcacggca
    12121 cgggttcccg gctgccagaa gtcactttcc cggatattca gggcagacag cgttttattt
    12181 tccacggcaa agcggaccat cttcaggtga ttccccgtcc atacggcatc tgccactgaa
    12241 gacagccccg ccggggcctg cagggtttcc tttgtcactg ggacctgata ccagcctgcg
    12301 ggtaattttc cgccacggac ggcctggctg atggtcacaa gcagggattc gtaaggcgtg
    12361 gacgtttccc acgcaccggc ttcttctccg gttccgcgca ggtcactgac cagctgaatg
    12421 gtacgccccg cgccttcacg gggaacggcc tgaatggaaa gattcagacc acgttctgtt
    12481 tccagaatga acgtaaaggg ctttttgttg acggtggcaa ccaccacacc gccggaggcg
    12541 gtctgctcat tattggtcag ggcaccatcc agactgttca cggcgataat ccggtcaccg
    12601 ggaacggtaa acagattcgg gctggtattg ctgatggcaa tactggcctg accaccgttc
    12661 accattggca ccacggtggg ggccagcgta ccgtttgcgg ccatcacgct gcaggaaaaa
    12721 aacaggctgc cgaatattat tgccgtatta ttttttctca tcgtctgttt ccccgaaatt
    12781 atccagccag gtcaccccgt tttcccgctt cagaataagg atgtaatgtt tgatatcagt
    12841 gaaaggtttt gaatcaccaa tccaggtttt aagcacacct cgaatttcca cacggccata
    12901 ctgaggccag acacgaacac tggtctggaa aaaggctgag ttcacgttat cgtttttaat
    12961 acgcttcgct tcttcagcca gaataacttt catctggttc tgtgcgcccg ggcggatata
    13021 ttgcagaagc gcctgatgtg aggcatcgac ggtttctgat gaaacattca gacggagggc
    13081 aataaatgac agcgccatct gctgtaaata agaggcgtcg gcactgttct gtgacacggc
    13141 aaagggggca ttaaatgcca ttggcgtcac ggcagtgcgt tgctcattct gaagacggta
    13201 gttattcacc ccctgaatga cgttaacaga gaggctgaga acaataagca ctgacataaa
    13261 tataaaggcg atggccatta cacgactggt acttaaacgg gcaccgtgtt ccatataaca
    13321 atcctggtat cagttctatt taatccactg ccggaaacac gaatcgggaa cattatgaaa
    13381 aataccgcgc agcagggctg ttggcatata ccagtaaatc aggtcacgta accaggaact
    13441 gccccgccct tttttcagtt ttttaatccc gaaataaacc agaaccgctg caccaatacc
    13501 gaacagatat ttcgatgttg tgatacccca gccaatacag attgctgcgg ggatcagttc
    13561 atccagtggc aggccaaacc agcggctctg gttggtcagt gtttccggga aacgatattt
    13621 cttaagttta ttctcgtctc ccgacatcgt tttatttcct gtcagaggcc aacgacggcc
    13681 atacccacag caataaatac agagatgatg gcaaaaccgg ccaggaactt gacgtttttg
    13741 gtcatcatgt acatgacagc accgaccaga acttcagcca gaacaaccca tttaacaaca
    13801 ctggagtcct taccgaaggt cgccttaacc gtggtgttac cgcttgccat caggtcctga
    13861 ccactgctgc cggcggccat cgccagctgc gggaagaaca tcatcagaac agcagccggg
    13921 atcactgcgc gagccaggcg aagcatattc agacgagtga atttagaaaa aaacgacttc
    13981 tttttgacgg gcgcagaagc accctgaaca cttaaaacag cattcataag ttacctcaat
    14041 ttcggataaa tgaataaaac tagagtgtat taaatgttat atcttcctct ttatctgcct
    14101 ccctgaatat ctccgagtta taaaaatcag gaaacttgat caaatgatca ataactctgt
    14161 ctgcagcctc atctgtttta caccatccac tacgattttt ggctgcaata agtagctggt
    14221 tagtttcatc atccagacgc attgtcagat acttaaccag cgacttacca tagatattcc
    14281 ctgccctgtt aaactttgga tagcggtgaa gatgatcttt aagtcttatt actgcctcaa
    14341 acgatcgact tcttccgctt ctgttacttg cctcaattaa tagactttcc acatctacag
    14401 gtaattttag ttttaccatc ttacctgttg cagaacgtgt accaaatctt ttcaataaca
    14461 cctcccgctg tttatcttct ggttaccact tatgtttaca gaataatcag aaaaggtaaa
    14521 tagacggaaa gtctat
//
LOCUS       DAMZII010000116        14519 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZII010000116 DAMZII010000000
VERSION     DAMZII010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.116"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..363
                     /locus_tag="POA77_003591"
     CDS             <1..363
                     /locus_tag="POA77_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6366959.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="POA77_003592"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="POA77_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDI6366960.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="POA77_003593"
     CDS             2185..3504
                     /locus_tag="POA77_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDI6366961.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3876..5258
                     /gene="dosC"
                     /locus_tag="POA77_003594"
     CDS             3876..5258
                     /gene="dosC"
                     /locus_tag="POA77_003594"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDI6366962.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5283..7682
                     /gene="dosP"
                     /locus_tag="POA77_003595"
                     /gene_synonym="pdeO"
     CDS             5283..7682
                     /gene="dosP"
                     /locus_tag="POA77_003595"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDI6366963.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDNKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7940..8521
                     /gene="ddpX"
                     /locus_tag="POA77_003596"
     CDS             7940..8521
                     /gene="ddpX"
                     /locus_tag="POA77_003596"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDI6366964.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYSLLADQFSCFISPGTQHVS"
     gene            8535..10085
                     /locus_tag="POA77_003597"
     CDS             8535..10085
                     /locus_tag="POA77_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6366965.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLGNDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRHLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10087..11109
                     /locus_tag="POA77_003598"
     CDS             10087..11109
                     /locus_tag="POA77_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6366966.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11106..12002
                     /locus_tag="POA77_003599"
     CDS             11106..12002
                     /locus_tag="POA77_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDI6366967.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11999..12985
                     /locus_tag="POA77_003600"
     CDS             11999..12985
                     /locus_tag="POA77_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6366968.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12978..13904
                     /locus_tag="POA77_003601"
     CDS             12978..13904
                     /locus_tag="POA77_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6366969.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13960..14391)
                     /gene="osmC"
                     /locus_tag="POA77_003602"
     CDS             complement(13960..14391)
                     /gene="osmC"
                     /locus_tag="POA77_003602"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDI6366970.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3539 a         3615 c         3702 g         3663 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagcg ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa ttcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacgagaa attagcgatt
     2221 agaagaccag cgatactggt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc gcctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcagag aactgaatag cactctgtct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgtgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgctgaa gtggtttccc gctatccggt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc acacaccatt aaaagcatta agccgggagt cgaatttggc
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtaccgg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccgcaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgttttgatt
     3841 ccgtctgatt tcccagcctt ataagggtgt gaattatgga gatgtatttt aaaagaatga
     3901 aagatgagtg gaccggactt gtcgaacagg cagatcctct cattcgtgct aaagccgcag
     3961 aaattgccct tgcgcatgct cattatctga gtatcgagtt ttatcgaatt gtccgcatcg
     4021 acccgcatgc cgaagaattc ttgagtaatg aacaagttga gcggcagttg aaaagtgcga
     4081 tggaacgctg gattattaac gtgctttctg cccaggttga cgatgtcgaa aggctaatac
     4141 aaatccagca taccgtcgcg gaagtgcatg cccgcatagg aattccggta gaaattgtcg
     4201 agatggggtt tcgggtgctg aaaaaaatcc tctatccggt aatcttctct tcggattatt
     4261 ctgccgcaga aaaacttcag gtctaccatt tctcgattaa cagtattgat atcgctatgg
     4321 aagtgatgac ccgcgcgttt acctttagtg acagtagtgc ctcaaaggaa gatgaaaact
     4381 atcgtatctt ctcgttactg gaaaacgccg aagaagaaaa agaacggcaa atagcctcaa
     4441 tactttcatg ggaaatagat attatctata aaatcctgct ggattctgat ttaggcagta
     4501 gtttgccttt aagccaggct gattttggcc tgtggtttaa ccataaaggt cgacattatt
     4561 ttagcggcat tgccgaagta ggccatatct cccgtctgat tcaggatttc gacggtattt
     4621 tcaatcaaac catgcgtaac accaggaatt tgaataacag aagtttgcgg gtgaagtttc
     4681 tattacagat aagaaatacc gtatcgcaaa ttattacctt gctgcgtgaa ttgtttgaag
     4741 aagtatcgcg ccacgaagtc ggtatggatg tactgacgaa attacttaac cgccgtttcc
     4801 tgccgactat cttcaaacgc gaaattgccc atgccaaccg gaccggtaca ccgctgtcag
     4861 tgctgattat tgacgttgat aaattcaaag agatcaacga tacgtggggc cataacactg
     4921 gcgatgaaat tctgcgtaaa gtctctcagg ccttttatga caacgtccgc agtagtgatt
     4981 atgttttccg ctacgggggc gatgaattta tcattgtttt gactgaagct tctgaaaacg
     5041 aaacgttacg taccgcagaa cgtattcgca gtcgggtgga gaaaaccaaa ctgaaagccg
     5101 caaacggcga ggatattgcc ctctcacttt ccatcggtgc cgccatgttt aatggtcatc
     5161 ctgactatga gcgcctcatt caaatagccg atgaagctct gtatatcgcc aaaagacgag
     5221 gtagaaaccg tgttgaactc tggaaagcca gtctttagat gcgccaggat gcagaggtaa
     5281 tcatgaagct aaccgatgcg gataatgccg ccgatggcat ttttttcccc gcccttgagc
     5341 aaaatatgat gggtgcggtg ttaattaacg aaaatgatga agtgatgttt ttcaaccccg
     5401 ccgcagagaa gctctggggg tacaaacgtg aagaagtcat tggcaataac attgatatgc
     5461 tgattccgcg ggatttgcgt cctgcgcatc ctgaatacat tcgtcataac cgtgaaggcg
     5521 gtaaagcgcg tgtcgagggg atgagtcggg agctgcagct ggagaaaaaa gacggcagta
     5581 aaatctggac ccgttttgcg ctatcgaaag tgagcgccga ggggaaagtt tattacctgg
     5641 cgctggtacg ggatgccagc gtagaaatgg cgcaaaaaga acagacccga caattgatta
     5701 ttgccgttga ccatctcgac cgaccggtga ttgtcctcga tccggaacgc catattgtgc
     5761 agtgcaatcg cgcatttacc gaaatgtttg gttactgcat tagcgaagcc agcggtatgc
     5821 agcccgatac actcctgaac attcctgaat tccctgccga taaccgcatt cgtttacaac
     5881 agttgctatg gaaaaccgcc cgcgatcagg acgaatttct gctgttgacg cgcaccggtg
     5941 aaaaaatctg gattaaagcc tctatcagcc cggtttatga cgtgctcgcg catctgcaga
     6001 accttgtaat gactttctcg gatatcaccg aagaacggca gatccgccag cttgaaggca
     6061 atattctcgc cgccatgtgc agcagcccgc catttcatga aatgggggaa atcatttgtc
     6121 gtaatatcga atctgttctc aacgaatcgc atgtttcgct gttcgcactg cgcaacggga
     6181 tgccgataca ctgggcgtca tcttcgcacg gtgcagaagt tcaaaatgcg caaagctggt
     6241 cagcgaccat tcgtcagcgt gatggtgcgc ctgcggggat cctgcaaatt aaaacctcgt
     6301 caggagcaga aaccagcgcc tttatcgaac gcgtggcaga tatcagccag catatggccg
     6361 cgctggcgct ggaacaggaa aaaagccgtc agcatattga acaactcatc caatttgatc
     6421 cgatgaccgg tctgccaaat cgcaataacc tgcacaatta ccttgatgac ctggtcgaca
     6481 aagccgtctc tccggtggtg tatctcatcg gtgttgacca tattcaggat gtgattgata
     6541 gccttggcta tgcgtgggcc gatcaggcat tgctggaagt ggtcaatcgc tttcgtgaaa
     6601 aactcaaacc ggatcagtat ctctgtcgta tcgaaggtac gcagtttgtc ctcgtgagcc
     6661 tggaaaacga cgtcagtaac attacccaaa tcgccgatga gctacggaat gtggtcagca
     6721 agccgataat gattgacaat aaacccttcc cgcttacctt gagtattggc atcagctacg
     6781 acgtgggtaa aaaccgcgat tacctgctct ccactgctca caatgcaatg gattatattc
     6841 gcaagaatgg cggtaacggc tggcagttct tcagcccggc gatgaacgaa atggtaaaag
     6901 agcgtttggt tttaggcgca gcgctgaaag aagcgattag caataaccaa ctgaaactgg
     6961 tttaccagcc gcaaatcttc gcagaaacgg gtgaactgta cggcatcgaa gcccttgctc
     7021 gctggcacga tccccagcat ggtcatgtgc ccccttcacg gtttattcct ctcgcagaag
     7081 agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc cgtcagttag
     7141 cagaatggcg tagccagaat attcatatcc cggcgttatc cgttaacttg tcggcgctgc
     7201 actttcgcag taatcagctg cctaatcagg tgtctgatgc aatgcaagcc tggggtattg
     7261 acggccacca gctgacggta gaaatcacgg aaagcatgat gatggaacac gataccgaaa
     7321 tctttaagcg cattcagatc ctgcgtgata tgggcgtggg cttatcggta gatgattttg
     7381 gtacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa atcaaaattg
     7441 acaaaagttt tgtcgatcgt tgtctgacgg aaaaacgcat cctggcctta cttgaagcca
     7501 ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggggtc gaaaccaaag
     7561 agcaatttga gatgctacgt aagatccact gtcgcgttat tcagggatat ttcttttccc
     7621 gcccccttcc cgccgaagaa attccaggct ggatgagcag cgtgttaccg ctgaaaatct
     7681 gacaaattcc tctcgcccgc actcgcgggt tttcctttaa ggtgacactg tcacttaaat
     7741 actgtgattt cagctacgat tccgggagat tccttcctta acttcccctc ttgcaccaaa
     7801 actgcactac gctgcacaaa tgttgaccaa aagttaaaaa atataacttt aagccagcat
     7861 tctggcgctt ctcccggcat ggcatgagat ctgcataagc ggaaagcgca gcaatttttg
     7921 tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag ccgtaatctt
     7981 ccctgatctg gagatcgaat tgaaatacgc ctgcgctgat aacatcacag gtaaagctat
     8041 ttatcagcaa gcgcgttgtc tgttacacaa ggatgcgatt accgcgctgg cgaaaagtat
     8101 cagcatcgcc cagctgtcag gcttacaact ggtgatttac gatgcgtatc gcccacaaca
     8161 agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg atgtgacggt
     8221 cggttctaat cacagccgtg gcacggcgat cgacctgacg cttcgtgatg agcacgggaa
     8281 catcctggat atgggcgcag ggttcgatga aatgcacgag cgctcccatg cctatcaccc
     8341 ttccgtcccg cccgccgctc agcgcaatcg cctgttgctg aatgcgataa tgactggtgg
     8401 cggttttgtc ggtatctcca gcgaatggtg gcacttcgaa ttaccccagg cagcgagtta
     8461 ctctctgctc gctgatcaat tctcttgttt tatatcccct ggcacacagc acgtcagttg
     8521 atcaggaaac tgtcatgaag agatcgatat cgtttcgtcc cacattgctc gcgctcgtcc
     8581 ttgccacaaa tttcccggtt gcgcacgccg ccgtaccgaa agatatgctg gtgattggta
     8641 aggccgccga tccacaaacc ctcgacccgg cggtaacaat agataataac gactggacag
     8701 tgacctaccc gtcttatcag cggctggttc agtacaaaac ggacggtgat aaaggctcaa
     8761 ccgacgttga aggcgatctg gcaagtagct ggaaagcgtc tgacgatcaa aaagagtgga
     8821 cgttcaccct gaaagataat gctaaatttg ccgatggcac acctgtcact gccgaagcag
     8881 taaaactttc ttttgagcgg ctactaaaaa tcggccaggg gccagcagaa gcatttccca
     8941 aagatttaaa gattgatgct cccgacgaac atacggtgaa gtttaccctt agccaaccat
     9001 tcgcaccgtt cctctacacg ctggggaatg acggtgcatc cattatcaat ccggcggtct
     9061 taaaggaaca tgcagcggat gatgctcgcg gcttcctcgc gcaaaatacc gccggttccg
     9121 gaccatttat gctgaaaagc tggcaaaaag gtcagcaatt agttctggtg ccaaatccgc
     9181 attaccccgg caataaaccg aactttaagc gagtatcggt aaaaattatc ggtgaaagtg
     9241 cctcccgtca cctgcagctc tcccgtggcg acattgacat tgccgatgcg ctgccggtgg
     9301 atcaactcaa cgccctgaag caggaaaata aagtcaatgt ggcagagtat ccgtcactgc
     9361 gcgttaccta tctgtatctg aataacagca aagcgcctct taatcaggcg gatctgcgtc
     9421 gggccatttc ctggtctacc gattatcagg gcatggttaa cggcattctg agtggtaacg
     9481 gaaaacagat gcgcggcccg attccggaag gcatgtgggg ctacgatgcg acggcaatgc
     9541 aatacaacca tgacgaaacg aaagccaaag ccgaatggga taaagtgacg agcaaaccca
     9601 ccagcctgac gtttctctac tccgataacg atccgaactg ggagcctatt gctttggcga
     9661 cacaatccag tctcaacaag ctgggcatca atgtgaagct ggaaaagctg gcgaacgcca
     9721 ccatgcgcga cagagtgggt aaaggtgatt acgacattgc gattggcaac tggagtccgg
     9781 attttgccga cccgtatatg tttatgaatt actggtttga gtcagacaaa aaaggtctgc
     9841 cgggtaaccg ctcgttctat gaaaacagtg aggtcgataa gttactgcgc aatgcgcttg
     9901 cgaccaccga ccagacgcag cgtacccggg actaccagca ggcacagaaa atcgtcattg
     9961 atgacgctgc ttatgtgtac ctgttccaga aaaactacca actggcgatg aacaaagagg
    10021 tgaaaggctt tgtgttcaat cccatgctgg aacaggtctt caatatcaat accatgagta
    10081 aataacgtga ccttctggag tattttacgc caacgctgct gggggctggt gctcgtggtg
    10141 gcgggcgtct gcgtgattac gtttattatc tcgcatctga tccctggcga tccggcgcgg
    10201 ttactggcgg gtgaccgcgc cagcgatgct atcgtggaaa atattcgcca gcaactggga
    10261 ctggaccagc cactgtatgt acagttttac cgctacgtca gcgacctgtt tcatggtgac
    10321 ctgggaacat ccattcgtac cgggcgtccg gtgctggaag agttgcgtat atttttcccg
    10381 gcgacgctgg agctggcttt tggtgccctg ctgctggcac tcctgattgg catcccgctg
    10441 ggcatactct ctgcagtctg gcgaaatcgc tggctggatc atctggtgcg aataatggcc
    10501 attaccggaa tctccacacc tgcgttctgg cttgggctgg gcgtcattgt gctgttttat
    10561 ggtcatctgc aaattcttcc cggcggcgga aggcttgatg actggctgga tccaccaacg
    10621 cacgttaccg gcttttatct gctcgatgcg ctgcttgaag gcaacggtga agtcttcttc
    10681 aatgcgttgc aacatctcat cttaccggca ttaacgctgg cgttcgttca cctgggaatt
    10741 gtcgctcgcc agatccgctc agcgatgctg gaacaattga gtgaagacta cattcgtacc
    10801 gcccgggcca gcggcttgcc cggctggtat atcgttttat gttatgcgct acccaatgcg
    10861 ttgatcccat cgattaccgt attgggtctg gcgctgggcg atttgttgta tggcgcagtg
    10921 ctcaccgaaa ccgtttttgc ctggcccgga atgggtgcat gggtagtaac atcaatacag
    10981 gcgctcgact tcccggcagt gatgggcttt gccgtcgtgg tttcatttgc ttatgtgctg
    11041 gtcaacctgg tggtggattt gctctatttg tggattgatc cgcgtatcgg acgtggaggt
    11101 ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa acgcgattta
    11161 acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc ggcgcagtca
    11221 tcattgtatt aatgctattg atgatgattt tttcaccgtg gctggcgacg catgatccca
    11281 acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg tttggcaccg
    11341 atgaagtggg acgcgatctg ttcagccgcg tactggtcgg cagtcagcaa tcaattctcg
    11401 ccggattagt cgtggttgcc attgcgggta tgattggttc gctactcgga tgtctatccg
    11461 gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt atgctgtcga
    11521 ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt ttatttaacg
    11581 ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcctggcg cgggggcaag
    11641 cattagttgt acgccagtat acctatgttc aggcggcgaa aacctttggt gcgtcgcgtt
    11701 ggcatctgat cagctggcat attttacgta actccctgcc gccgctgatc gtgcaggcat
    11761 cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt ggcctgggtg
    11821 ctcaacaacc gagtgctgaa tggggcgcga tggtagcgaa tggtcgcaac tatgtactcg
    11881 atcagtggtg gtattgcgca tttccggggg cagcgatttt gcttaccgcc gttgggttta
    11941 atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga aagcagtcat
    12001 gacccaaccc gttctggaca ttcaacagct gcatttgagt ttccccggtt ttaacggtga
    12061 tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc ggcgaaattg tcggtctggt
    12121 gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc tgctacagac
    12181 gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagacgatg ttcttaacgc
    12241 tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc
    12301 gatgaccgcc ctcaatccga cacgtcgaat aggtcttcag atgatggacg tgatccgcca
    12361 tcatcaacca ataagtcgtc gggaagccag agctaaagcg attgccctgc tggaagagat
    12421 gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt caggtggtat
    12481 gcgccagcgg gtaatgattg cgctggcatt ctcctgcgag ccgcaattga ttattgccga
    12541 cgaaccgact acggcgctgg acgtcacgct acagttgcag gtactgcgtt tgcttaaaca
    12601 taaagcccgc gccagtggaa ctgcggtact gttcatcagc catgatatgg tcgtggtgtc
    12661 gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa gcggcgtgac
    12721 ggcagacatt atccatcatc cccggcatcc gtataccatt ggtttgctgc aatgcgcacc
    12781 agaacatgga gtaccacgcc agccattacc cgccattcca gggacggtac caaacctcac
    12841 ccatttgcct gaaggctgcg cttttcgcga tcgttgctat gcggcaggtg cacagtgtga
    12901 aaacgtcccg gcgctgacag cgtgtggtga caacaaccag cgctgcgcct gttggtatcc
    12961 gcagcaggag gtcataagtg tctgacacgt tattaacgtt acgcgacgtc catatcaatt
    13021 tcccggcccg taaaaactgg cttggtaaaa ctacggaaca tgttcatgcc atcaatggta
    13081 ttgatttaca gatccgccgt ggtgaaacct tagggatcgt cggcgagtca ggctgcggca
    13141 aaagcaccct cgcacagctt ttaatgggca tgctgcaacc gagccacggg cagtacatcc
    13201 gttctggctc acaacgcatt atgcagatgg tgtttcagga cccgctctct tcgctcaatc
    13261 cgcgcttacc ggtgtggcgc atcatcacag aaccgctctg gatagctaag cgtagtagtg
    13321 aacaacagcg gcgagcgttg gcagaggagc tggctgtgca ggtgggtatt cgtccggagt
    13381 atctcgaccg cctgcctcat gcgttctccg gcgggcagcg gcaacgtatc gccattgcca
    13441 gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctct gcactggata
    13501 tctccgtgca ggcgcagatc ctcaatttac tggtaacgct acaggtaaat cgcgggctga
    13561 cctatgtgct gatctcacac aatgtctcgg tgattcgtca tatgagcgat cgggtagcgg
    13621 tgatgtatct cgggcagatt gtagaactgg gcgacgcgca gcaggtgttg acggcacctg
    13681 cacatccata cacccgatta ttgctggatt ccctccccgc cattgataaa ccgctggagg
    13741 aagaatgggc attacgtaaa acggatctgc cgggaaaccg cacgttgccg caaggctgtt
    13801 ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa tcattaacaa
    13861 taagggagga cggacgtgag atccggtgct ggcgggcgct gtagagatgc taacaacgca
    13921 tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca actggtaatc
    13981 aagcgtaatt tccgctttca gtacctgaga aaccgggcat cctgcttttg ctttctggat
    14041 gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct tcagtgcgat
    14101 tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg tggtatcaat
    14161 cgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg agaaacatgc
    14221 ggcatgcgct gcgccaatca gttcttcagg gttggttcct ttttcgcctt caaaacgcgt
    14281 gttaaatcca tacggctgtt ggttcagcac gccgctctcg gtggatactg ttcccttccc
    14341 gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca ttgttgctct
    14401 cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg ataagcagga
    14461 tattccgaat aaaatacggc agaaaatatt acttctgcac ttaaattaaa ttcccacaa
//
LOCUS       DAMZII010000117        14515 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZII010000117 DAMZII010000000
VERSION     DAMZII010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.117"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..704)
                     /locus_tag="POA77_003603"
     CDS             complement(<1..704)
                     /locus_tag="POA77_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDI6366971.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLARE"
     gene            complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="POA77_003604"
     CDS             complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="POA77_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDI6366972.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="POA77_003605"
     CDS             complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="POA77_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDI6366973.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="POA77_003606"
     CDS             complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="POA77_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDI6366974.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="POA77_003607"
     CDS             complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="POA77_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDI6366975.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="POA77_003608"
     CDS             complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="POA77_003608"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI6366976.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7319..8554
                     /gene="moeA"
                     /locus_tag="POA77_003609"
     CDS             7319..8554
                     /gene="moeA"
                     /locus_tag="POA77_003609"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI6366977.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDI
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVISKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8554..9303
                     /gene="moeB"
                     /locus_tag="POA77_003610"
     CDS             8554..9303
                     /gene="moeB"
                     /locus_tag="POA77_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDI6366978.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAAMIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9391..10053)
                     /gene="fsa"
                     /locus_tag="POA77_003611"
     CDS             complement(9391..10053)
                     /gene="fsa"
                     /locus_tag="POA77_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI6366979.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLATIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            10184..11083
                     /locus_tag="POA77_003612"
     CDS             10184..11083
                     /locus_tag="POA77_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDI6366980.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11089..13521
                     /locus_tag="POA77_003613"
     CDS             11089..13521
                     /locus_tag="POA77_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDI6366981.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13667..14482
                     /gene="ybiV"
                     /locus_tag="POA77_003614"
     CDS             13667..14482
                     /gene="ybiV"
                     /locus_tag="POA77_003614"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDI6366982.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
BASE COUNT         3542 a         4002 c         3631 g         3340 t
ORIGIN      
        1 ttttcacgag cgagttgatt cttggcaatg atctcatgac cttgtttcaa aaatagaact
       61 tcatcattag cgctgatttg atcaagatac tcgttcagat accgccagtt taaatcgaca
      121 tgaattttat ctaccgctaa caaatcgtga tctttgccgt tgatacgtag cgtaaaagag
      181 atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag
      241 tcatctgaag ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc
      301 cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca
      361 ttcgccctcc gtgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct
      421 aaaaatggca tcatttccat tttcagaatc acggcgcgtt gaatctcctc tgtatttttt
      481 aataaataca gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag
      541 ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat
      601 accagttgat agttaatgat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat
      661 aaaattatca gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca
      721 aaataactat ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc
      781 aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattg
      841 cctgtgaggt gattaccctt tcaagcaata ttcaaacgta actatccttt aattttcgga
      901 tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt cagaaaaata
      961 gccagggccg gaaaaacagc gacatgcggc gcgataacca tatccgctcg tgcctcattg
     1021 agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg
     1081 gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa acaccacgat agacgagacg
     1141 gtcccaggca agatatgacg caataaaatg gtcatatcgc tggcaccaat actgcgtgct
     1201 gactcaataa aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac
     1261 gcggggatgg aaaaaatggc gactgcgata atcacgttag caatgccgct tcccaacacc
     1321 gcaacaacag cgatcgccag taaaataccc gggaaggcaa acagcacatc gcaaatgcgc
     1381 atgatcagcc gatcccacca gccttcataa tatccagcca gcaagcccag caacgtccct
     1441 atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc
     1501 aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa
     1561 ggtccgttat tcagattgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag
     1621 cgtgcaaaaa tggccaccac aatcaataaa ataacgaata atgcggcggt catcgccata
     1681 tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgcacctg gtcaggtttg
     1741 accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact
     1801 tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa
     1861 taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta
     1921 cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aactttctcc accacgatag
     1981 aaccaccgag caaaaagcca aactgtaagc ccatcatggt cactaccggg atcatcgcgt
     2041 tacgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac
     2101 gcatataatc ttcgcttaaa acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg
     2161 ctgccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc
     2221 ccacggtagg cagccagccc agttcaacgg agaatacctg aattaaaagc atccccagcg
     2281 caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac
     2341 gattacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca
     2401 tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaaccg
     2461 gacgacgcga caccatcgac aggccaaaat ccccctgcac agcattgctg atatagtgcc
     2521 agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct
     2581 gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atatgcacaa
     2641 ataaaaacac cagcaccgag acgataaaca gcgtcggaat caaccccagt aatcgtttga
     2701 taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct
     2761 gaagccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag
     2821 tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct gcgccgcctt
     2881 atacaagcgg gtcttttccg ccggatcatt agttttcagt gcctgagcca ggaagtcatc
     2941 cacctgttta ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt tttgcgaggc
     3001 aaacaacggc gatagtgccc agtcggcttc gccggttgaa gccgaccagc cagtgtagaa
     3061 catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc
     3121 atccatcgca gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg
     3181 cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt
     3241 gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt
     3301 ataactttgc gcgtaggcga tacttggcgg taccacacca gtagctggcg ttgcatagcc
     3361 cgcaaaggca actttcacca gcgccggacg gttaatggcg taattcagcg cctcacggac
     3421 cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct gcataattga
     3481 cggactggcc atcaactcga tatttttgtt tttctccagc agtgtggcct gctcgtaggg
     3541 aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc
     3601 cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc agtaacccgc
     3661 gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat acggtccggt
     3721 tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg gtgaaatcat
     3781 cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt tgagagtaat
     3841 ctttactgtt gttggatcga tcgcctccgt tttggcaata ttcttataca ggttatagcg
     3901 tttaagatga ttcgccggat cgctggcccg gtccagattc gcttttaccg ccgctgcgtt
     3961 gaaatcggtg ccatcctgga atttaattcc ttcccgcaat ttcacggtgt atgtaaggcc
     4021 gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct ctttatccag
     4081 accaaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtgt catttgcgtc
     4141 atacggatcg agcgtggtga agttcgatcc taccgccacc accacatctt tggcagcgaa
     4201 tgcacaagat gccatcaacg ctgtcgcaat gcccaacgcc actaacccac tacggtgtac
     4261 agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt
     4321 gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt
     4381 cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt
     4441 gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgctgcg
     4501 ggttttcgaa gaccgcgcgc cgtggaccaa tttcaacaat ttgtccgaga tacatcaccg
     4561 ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac agatacgcaa
     4621 tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat
     4681 ccagcgccga gacggcttcg tcggcaatga tcactttcgg attcaatgcc aacgcgcgag
     4741 caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt
     4801 caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgct tctttacctg
     4861 gcaataaacc gtgtacacgc agcggttcga taatcgaatc accgatggtc tgacgtgggt
     4921 ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt
     4981 tgccgggtga caaggtatcg attctctgac cgttaaagat aatttcgccg ccctgcgatt
     5041 cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca
     5101 gcgatagcgt ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc
     5161 gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc
     5221 gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggggcc tgtttcgctg
     5281 gatgttcaag cgatatcaac gggaaacgtc ggggataatc taaccctttc atcgcaccaa
     5341 gttgcggaac agcagctaac agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct
     5401 gttcgacggt acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa
     5461 tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt
     5521 gcaatacttt gattaattgc aggatctgcg cctgaatagt gacatccagc gcggtggttg
     5581 gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct
     5641 ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa
     5701 tgcgtacctg atccagcatc cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct
     5761 gatgcagacg aattgattcg gcaatctgtt cacccacagt aaataccggg ttcagcgatg
     5821 tcatcggctc ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc atttgtgcag
     5881 cgctctgctc gctaagttca atcacttcgc gactgcgccg ctgcaacagc attttatcgc
     5941 actgtactaa accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca gtcactgact
     6001 taccggagcc ggattcgcca acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga
     6061 gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc agattttcaa
     6121 ccgccagcac attaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca
     6181 cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg
     6241 gtgttaaacg gtagcgcgac attcccttca tggtcgatag cgattaagcc accgctaccg
     6301 ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg
     6361 ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgccc
     6421 gtgccggtac aagaaaccgc cacactggcg ttattggcgt agcatccggc acccactaag
     6481 ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc
     6541 gccaaattgc cgtctaaatc caacgccacg gcccccacgg tgcccatttt ctgtttttca
     6601 tccagtggcg caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt
     6661 tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat gccacgagca
     6721 aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc
     6781 agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc
     6841 agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca
     6901 gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc
     6961 acatccagcg cactttcgcc cgcttccagc attttctgcc cggtttcaac aatggcagac
     7021 aacgcctcaa tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct
     7081 gcgccaccat gaattgcaat gactgctttg cccatagtat tcatccatta agggtggctc
     7141 gccacttttg ctataaatat cagaattttt atgaattact cattcaattt ttgaatatag
     7201 aaagatggat atggcatgta aaggcaggac ccagcgctac gcccgaggtg gcatacagtc
     7261 tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat
     7321 ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt
     7381 caccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc gtattctggc
     7441 gagcgatatc gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa tggacggcta
     7501 cgcggtgcgt ttagccgata ttgcctccag gcaaccgctg cccgttgccg gtaaatcctt
     7561 tgccggtcag ccgtaccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg
     7621 tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gagcagactg aacaaacgga
     7681 caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga
     7741 agatatctct gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta ccgctgaact
     7801 gccagtgatt gcttcactgg ggattgccga agttccggtg attagtaaag tgcgtgtagc
     7861 gcttttttct accggtgatg aactccagtt gcccggtcag ccactgggcg atggccaaat
     7921 ctacgatacc aaccgtctcg ccgtacacct gatgttagaa cagttgggat gcgaggtaat
     7981 caacttaggg attatccgcg acgatccaca tgccctgcgc gccgcattta ttgaagccga
     8041 cagccaggcg gatgtggtga tcagttccgg cggtgtttca gtgggtgaag cggattacac
     8101 caaaaccatt cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccggg
     8161 taaaccgttc gcgttcggta aactttctaa tagctggttc tgcggcctgc cgggcaaccc
     8221 ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg
     8281 taacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa
     8341 aacgccaggt cgccttgatt tccagcgcgg cgtgctgcaa cgcaacgccg atggcgaact
     8401 ggaagtgacg accaccggac atcagggttc acatatattt agctccttta gcctcggcaa
     8461 ctgctttatc gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt
     8521 agaaccattt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg
     8581 ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgcta
     8641 aaagattctc gtgtgctggt cgtcggcctc ggtggactcg gctgcgcagc ctcgcagtat
     8701 ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctcg
     8761 aatctgcaac gccagacgct gcacagtgat gccacggtcg ggcaacctaa ggttgaatcc
     8821 gcacgtgacg ccctggcgcg gatcaacccg catattgcga ttacgccagt caatgcactg
     8881 ctggatgacg ctgaacttgc tgcgatgatt gctgaacacg atctggtgct cgactgtacg
     8941 gataacgttg cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtaccgctg
     9001 gtttccggcg cggcaattcg tatggaaggt caaatcacgg tcttcactta tcaggacggt
     9061 gaaccatgct accgctgcct cagccgtttg tttggtgaaa atgcattaac ctgcgtggaa
     9121 gcaggcgtaa tggcaccgtt gattggcgtg attggttcgt tgcaggcaat ggaagcgatc
     9181 aagctgctgg caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg
     9241 acctgtcagt ttcgcgaaat gaaactgatg cgtaatccgg ggtgtgaggt gtgcgggcag
     9301 taattgccac ctccaggcct gataagacgt ggcaggcatc ggtgcactcc gccacgtcat
     9361 cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg
     9421 ccagtcctgc tcaaacttcg ccacagcggc atcaaccgcc ggatagctaa tcatctgctg
     9481 cgccacatcc agcggcagcg taattgattc acatcctgcc agtaagcagt ccagagcctg
     9541 acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa
     9601 ctggtgtaag tcggtcacag tctgaatacc gctaccgccc tgagcatcaa tacgattaac
     9661 gtaaggcgca acatattccg cacctgccag cgccgacagc aacccttgtg ccgcaccata
     9721 taccgcggtt cccagcgtcg gaatcccttc cgcttttaac atcttaatag ttgccaaccc
     9781 ctcggcggtc accggaactt tcaccacgat atccgcaata atagagcgca atttacgtgc
     9841 gtcattcacc atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc
     9901 gcccatcgct tcatgaagtt ccggaagcac aacctccagc ggttttttac ccgcggcgat
     9961 aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc
    10021 aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc
    10081 tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc
    10141 attcgaaagt attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca
    10201 gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc
    10261 gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta
    10321 tgacccacga ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc cggaagtgat
    10381 tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac
    10441 ggcgttaacc gactgctgtc cgacacaggc attaaccgtg tgtggtgaag tgaaaagcgt
    10501 tgaggagatc atgacgaccg ttctgcgcga taaaccgttt tacgatcgca gcggcggcgg
    10561 tttaacgctt tcgggtggtg aaccctttat gcagccggaa atggcgatgg cgctactgca
    10621 agccagccac gaggccggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa
    10681 atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc
    10741 cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agggtgctgg ataacctgaa
    10801 aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa
    10861 tgccgacgaa acctctgtga aagccattac cgattttgcc gccgacgagc tgcacgttgg
    10921 cgaaattcat ttcctgccct accacacgct gggcatcaac aaatatcact tacttaatct
    10981 gccctatgac gccccggaaa aaccgcttga tgcgccagag ctgctcgatt ttgcccagca
    11041 gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg
    11101 aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg
    11161 aaaccgccag tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc
    11221 gataagccga tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc
    11281 atctggatca aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg
    11341 ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat
    11401 cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc
    11461 ccgtggtggc gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa
    11521 aaaggtctgc tggcgaccgg aatcattaaa gcggaaggca atatgacctc cggcgatgcg
    11581 cacctggcgg tgaatttccc gctgctgctg gaaaaaggtc ttgatggtct gcgtgagaaa
    11641 gtagcggaac gtcgctcgcg catcaatctg acggtgctgg aagatttaca tggcgaacaa
    11701 ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct
    11761 gccctggcgc gtgaaatggc tgctaccgaa actcgcgaaa gccgtcgcga tgaactgctg
    11821 acgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg
    11881 ctgcaactgt gttacttcat ccaattgatt ttgcagatcg aatctaacgg tcactcggta
    11941 tcgtttggtc gtatggacca gtatctctac ccgtactatc gccgcgacgt tgaactcaac
    12001 cagacgctgg atcgcgaaca cgccatcgag atgctgcata gctgctggct gaaactgctg
    12061 gaagtgaaca agatccgctc cggctcacac tcaaaagcct ctgcgggaag tccgctgtat
    12121 cagaacgtta ccattggcgg acaaaatctg gttgatggtc aaccaatgga tgcagtgaat
    12181 cccctctctt acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc
    12241 agcgtgcgtt accatgcagg aatgagtaat gatttcctcg acgcctgcgt acaggtgatc
    12301 cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc ggaatttatt
    12361 aaactcggta ttgaaccgca ggacgcttac gactacgcag cgattggttg tatcgaaacc
    12421 gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc
    12481 gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca
    12541 caagaaaaag cgttgtcggc aggcaacttt aacaatttcg atgaagtaat ggacgcgtgg
    12601 gatacgcaaa tccgttatta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc
    12661 atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag
    12721 cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt
    12781 ggcattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtatt tgaacagggc
    12841 gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat
    12901 gagcaactgc gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact
    12961 gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat
    13021 aatccgcgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcatctatc
    13081 tccgctaacg taccgtttgg cgcgcaaact atggcaacgc cggacgggcg taaagcccac
    13141 accccgctgg cagaaggcgc tagtccggcc tctggtactg accatctcgg ccctactgcg
    13201 gtcattggct cggtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac
    13261 cagaaactga atccggcaac gctggagaac gaatctgaca agcagaagct gatgatcctg
    13321 ctgcgcacct tcttcgaggt gcataaaggc tggcatattc agtacaacat cgtttcccgc
    13381 gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc gcgatctggt agtgcgtgtc
    13441 gcgggttatt ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgct
    13501 cgtactgaac atatgctgta atctcttcgg cccggctgcg gcgtcgccgg gctaaatcat
    13561 tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat
    13621 tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt
    13681 tatcgtcaca gacatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg
    13741 ttttatggcg caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg
    13801 taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt
    13861 cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac
    13921 ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt
    13981 cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact
    14041 gatggcaaaa cactaccatc gcctgaaagc tgtaaaagat tatcaggaga ttgacgacgt
    14101 actgttcaag ttttcgctca acctgccaga tgaacaaatc ccgttagtga tcgacaaact
    14161 gcacatagcg ctcgatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct
    14221 gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct
    14281 gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat
    14341 ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta
    14401 cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggcgg tgctggataa
    14461 cacatcccct tttaacagct gacctctcta ccggagccgt gcgctccggt tttcc
//
LOCUS       DAMZII010000118        14402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZII010000118 DAMZII010000000
VERSION     DAMZII010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.118"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            81..1424
                     /locus_tag="POA77_003615"
     CDS             81..1424
                     /locus_tag="POA77_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6366983.1"
                     /translation="MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQM
                     NYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPI
                     NGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPF
                     LVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRL
                     ASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
                     LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHR
                     VGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLT
                     GFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1605..3095
                     /locus_tag="POA77_003616"
     CDS             1605..3095
                     /locus_tag="POA77_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6366984.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3138..3641
                     /gene="yeaK"
                     /locus_tag="POA77_003617"
     CDS             3138..3641
                     /gene="yeaK"
                     /locus_tag="POA77_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDI6366985.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3642..3746)
                     /locus_tag="POA77_003618"
     CDS             complement(3642..3746)
                     /locus_tag="POA77_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6366986.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3916..4362
                     /locus_tag="POA77_003619"
     CDS             3916..4362
                     /locus_tag="POA77_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDI6366987.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4319..5140)
                     /gene="nimR"
                     /locus_tag="POA77_003620"
     CDS             complement(4319..5140)
                     /gene="nimR"
                     /locus_tag="POA77_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDI6366988.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            5237..6418
                     /gene="nimT"
                     /locus_tag="POA77_003621"
     CDS             5237..6418
                     /gene="nimT"
                     /locus_tag="POA77_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDI6366989.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6473..6820
                     /locus_tag="POA77_003622"
     CDS             6473..6820
                     /locus_tag="POA77_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDI6366990.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6842..7096)
                     /gene="yoaF"
                     /locus_tag="POA77_003623"
     CDS             complement(6842..7096)
                     /gene="yoaF"
                     /locus_tag="POA77_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDI6366991.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7279..8304
                     /gene="dgcP"
                     /locus_tag="POA77_003624"
     CDS             7279..8304
                     /gene="dgcP"
                     /locus_tag="POA77_003624"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDI6366992.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8337..8435
                     /locus_tag="POA77_003625"
     CDS             8337..8435
                     /locus_tag="POA77_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDI6366993.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8438..8512
                     /gene="yoaJ"
                     /locus_tag="POA77_003626"
     CDS             8438..8512
                     /gene="yoaJ"
                     /locus_tag="POA77_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDI6366994.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8571..8819)
                     /locus_tag="POA77_003627"
     CDS             complement(8571..8819)
                     /locus_tag="POA77_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6366995.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9046..10254)
                     /locus_tag="POA77_003628"
     CDS             complement(9046..10254)
                     /locus_tag="POA77_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6366996.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            10262..10693
                     /gene="tnpA"
                     /locus_tag="POA77_003629"
     CDS             10262..10693
                     /gene="tnpA"
                     /locus_tag="POA77_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDI6366997.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(10709..10897)
                     /locus_tag="POA77_003630"
     CDS             complement(10709..10897)
                     /locus_tag="POA77_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDI6366998.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10901..11260)
                     /gene="yeaR"
                     /locus_tag="POA77_003631"
     CDS             complement(10901..11260)
                     /gene="yeaR"
                     /locus_tag="POA77_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDI6366999.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11433..12071)
                     /gene="leuE"
                     /locus_tag="POA77_003632"
     CDS             complement(11433..12071)
                     /gene="leuE"
                     /locus_tag="POA77_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDI6367000.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(12198..13121)
                     /gene="dmlR"
                     /locus_tag="POA77_003633"
     CDS             complement(12198..13121)
                     /gene="dmlR"
                     /locus_tag="POA77_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDI6367001.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13224..14309
                     /gene="dmlA"
                     /locus_tag="POA77_003634"
     CDS             13224..14309
                     /gene="dmlA"
                     /locus_tag="POA77_003634"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDI6367002.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
BASE COUNT         3598 a         3518 c         3377 g         3909 t
ORIGIN      
        1 cgttaacgaa aattgatcct ggtcaaaact atatatctct gccatcaata aaatccagca
       61 ctcacattgc tctccttttt atggtttcta tgggtacaca aaaattaaaa gctcaaagct
      121 tttttatttt cagtttattg ctgacgttaa ttttattttg catcaccaca ttatataacg
      181 aaaacacaaa tgtaaaactc atcccacaga tgaattacct gatggttgtt gtggctttat
      241 ttttccttaa cgccgtcatt tttcttttca tgttaatgaa atatttcact aacaaacaaa
      301 ttttaccaac actcatttta agccttgcat ttttaagtgg ccttatctat ttagttgaaa
      361 ccattgtaat tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg
      421 atgtttctat tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcac
      481 tcttttgtta tggaaaagac aacatccttg acaacaataa gaaaaaaacg ggaatcctgt
      541 tgctggcgct gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata
      601 acgctgacta ttctttgtat gtcgtcgact actgtccgga caaccatact gcgacctggg
      661 gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca
      721 tgcgtacacg attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc
      781 tatgctgcaa cttacttcta ctgactctgg atgagtataa ttacaccatc tggtatatca
      841 gtcgcgggat tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt
      901 ttcaggaact gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata
      961 atcggcgcta ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact
     1021 tctgtgtcat gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc
     1081 gtgtgggtga taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccctg
     1141 atgatatttt agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata
     1201 gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct
     1261 ttagtaacag atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa
     1321 cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag
     1381 cgaaaagcga ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag
     1441 agccatgctt tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc
     1501 tccccagcgg cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt
     1561 tatctggttt tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata
     1621 gaatgctccg gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt
     1681 ttgaacttgt cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag
     1741 cagactcctc ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc
     1801 gcgctctcgc tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg
     1861 cttttgagtc tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct
     1921 tacgcggcac actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac
     1981 tattacccgc ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag
     2041 gtcttgggat ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct
     2101 atttttatga gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt
     2161 caggaagtat tggtatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat
     2221 tgtcgagcat ttaccaagat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg
     2281 tgtatgttgc agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc
     2341 ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa
     2401 ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt
     2461 atgtcagtta cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc
     2521 tttactttat cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt
     2581 tgctgaatat ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta
     2641 gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt
     2701 tgcagaaact ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt
     2761 taaagcaaat aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttattag
     2821 ctcaggcgat taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg
     2881 aattctgcat cattcttgtc gattcgacgc cgcaaattgc agcacaactg cctgaacgta
     2941 tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat cggcttctct tccggtattt
     3001 acgcgatgaa agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat
     3061 atgtcaataa gcagaataaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt
     3121 aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa
     3181 tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa gcggtaggta
     3241 aatgtgaagc ggtgtcagaa attcgtggca ccgctttagg ccagggagct aaggcactgg
     3301 tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gctgccgatc
     3361 aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca
     3421 gcccggcgga agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct
     3481 tccatccaaa actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg
     3541 ccttcaatgc agggatgctg gataaatccg ttattttgaa aaccacggat tatttgcgca
     3601 ttgcgcaacc agaactgctc aatttccgcc gcactgcgta actagcctgt ccgttcaata
     3661 agcagaacgg acaaaaccag cacaacagca atggcaaaaa acgatgacgt gataatcagt
     3721 gtctcgacga acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta
     3781 ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac
     3841 gtaaagattt tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt
     3901 ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg
     3961 ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc
     4021 gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata
     4081 atcattctga cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc
     4141 ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc
     4201 tcctggctgg gtgggcgcgg cgtgacctta atgggcagcc agccgcaact ggtcgccggg
     4261 ctgctggtag gcactgtttt aggcgtggcg ctgtttcgcg gcgtaccggt cggcccgctt
     4321 attgccgcgg gtcttgtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc
     4381 ggcctggcgt ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat
     4441 accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagtgcca
     4501 taatcagctg taactgctga cgccactggc gaaaactcaa ccccgtctct tttactatca
     4561 gtcgcgcaag attgcgttca ctcatcgcaa aaaagccagc ccattgccct aatgaccccc
     4621 actcgacggg ccctttcgcc atcatctcca ccatcgtgcg gattttagga tgagaagaaa
     4681 cgggcaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta
     4741 atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca
     4801 attcccggca taagggcgat attttcaatg tgcaacatgt tgtcggcatt gtcactgcgc
     4861 tgggttcgat aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca
     4921 ccccgcccgg tatccaaacc gcatattgcg gcggcaccat ccacaaagca ttttccaccg
     4981 tacaggtaat tgcaccatgt aatgctagta tcagctgtcc tttgcggtgc tgatgtacag
     5041 gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc
     5101 ggtcgggttc atagccattg agattcaacc tgtgcatcat tttgtccgaa tttagcgata
     5161 atttgtcatt ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg
     5221 taacaatgag aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct
     5281 tatcgctggc attctgatga ttgccacaac attacgcgtc acctttaccg gcgcagcgcc
     5341 gttactggat acgattcgtt ccgcttactc gctgacgaca gcgcaaaccg gcttattaac
     5401 caccctgcca ttattggcct ttgcgctaat ctcacctttg gctgccccgg tggcgcgacg
     5461 ttttggtatg gaacgtagcc tgtttgccgc gttacttttg atctgtgcag gtatcgcaat
     5521 tcgttctctc ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtgggat
     5581 tgcattaggc aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc
     5641 cagacttacc ggcgcatatt ccctgacaat gggagctgca gcggcactgg gatcggctat
     5701 ggtcgtgccg ctggctttga acggttttgg ctggcaaggc gcgttgttca tgctgatgtg
     5761 ttttcctctg ctggctcttt ttttatggct gccacagtgg cgaagccaac aacatgcaaa
     5821 tttgagtacc tcgcgcgcct tacatactcg gggtatctgg cgttcgccgc ttgcctggca
     5881 ggtcacattg tttcttggga tcaactcact ggtctattac gtgattattg gctggcttcc
     5941 ggcgatcctc atcagtcgcg gctatagcga agcacaggcg ggttcactgc atggtttgct
     6001 gcaactagcc acagcagctc ccggtttgct gatccctctt ttcttacatc atgtgaaaga
     6061 tcagcgtggt attgcagcgt tcgttgcctt gatgtgcgca gtgggcgctg ttgggctctg
     6121 ctttatgcca gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac
     6181 aatgatactg gggttgacgt tcattggtct tcgggcaagt tctgcgcatc aggcggcggc
     6241 actctcgggg atggcacaat ccgtcgggta tttgctggca gcctgtgggc cgccgctgat
     6301 gggtaaaata cacgatgcta acggtaactg gtccgtacca ctgatgggtg ttgcaatact
     6361 ttcactactg atggcgattt tcggactttg cgccgggaga gacaaagaaa ttcgctaata
     6421 tccggtgcta tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat
     6481 tcagtgcaaa cgcgtttatg atccggctga acagagcgat ggttatcgcg tactggtcga
     6541 ccgcctctgg ccgcgcggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga
     6601 aatcacgccg tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac
     6661 ctttcgcgag caatatcttg cagaactggc gcaacacgag caagaaggaa agcggctggc
     6721 ggacatcgcc aaaaaacagc cgctgaccct gctctacgca gcaaaaaaca ccacgcagaa
     6781 ccatgcgctg gtgctggccg actggctacg tagcttgtga ttttagtaca gcatccggcg
     6841 gttatttttc accagccgga tggtcccgtc gccacaatgc ccattcatca atcgtttcac
     6901 cgcccggtaa tttgcaattg ttgctgaccc cttgcgctgt cgacactgga atgagcgtcc
     6961 cgcccttctg ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttag
     7021 gtgcttctgg ctgagaagag gttgaacaac cagccaggac cagcaagcaa ggcaaagcaa
     7081 aactgataat tttcatttat tgatctcaca tatttatcca gacttagagt atcgcggtat
     7141 cgttttgttt tgcagcacta tttttattac attcactcaa aacatattac gtcttgtttc
     7201 atctttgttg gtgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc
     7261 gtatcctgga gttgtttcgt gtcagatcag attatcgccc gcgtctcgca atcccttgcc
     7321 aaagaacagt cactggaaag tctggtccga cagcttctgg agatgctgga aatggtcact
     7381 gatatggaat caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg
     7441 tttgcccgta acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac
     7501 tcgttatgca aacgcgccat tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt
     7561 tggggcgact gtattgcggc acgcaatctt ggcatcacca catttctgag cacgccaatt
     7621 cacttaccgg atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag
     7681 tggagtgaac gcgcggaaca ggttttacag ttattcgctg gactgattgc acaatatatt
     7741 caaaaagagg cgctggttga acagctgcgc gaagccaatg ctgcgctgat tgcgcaatcg
     7801 tataccgact cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa tctgacgacg
     7861 ctgttttctc tcgcccggca tcttaaccat aagataatga tcgcgtttat cgatctggat
     7921 aacttcaaat taatcaatga ccgttttggt cataatagtg gcgatctgtt tctcattcag
     7981 gttggcgagc gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggcggt
     8041 gatgagtttt tggttgtttc actaaacaac gagaatgcgg atatttcgtc gctgcgagaa
     8101 cgtattcaac agcaaatacg tggagaatat cacttaggtg atgttgattt gtattatccc
     8161 ggtgccagtc ttggcatagt agaagtcgat cctgaaacaa ccgatgcaga cagtgccctg
     8221 catgctgccg atattgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc
     8281 gcgcatccag cgctacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc
     8341 gaatcggtat tatttttccg gttgtaatct ttattacagc ggtcgtattt ttagcatggt
     8401 tttttattgg cggctatgct gccccgggag cataaagatg aaaaaaacaa cgattattat
     8461 gatgggtgtg gcgattattg tcgttctcgg cactgagctg ggatggtggt aacgtcacct
     8521 ctaaaaaata gcaaaggctg cctgtgtgca gcctttgtgc aatttaagcg ttaactttta
     8581 atcttcctgt agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca
     8641 aaattgaagc catcgacttt accaaagcca aacagtgtgc tgatccatcc gccgactacg
     8701 gcaccgacta tccccagcag aatagtcata aagaatccac caccatcttt acctggcatg
     8761 atccacttcg ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt
     8821 gtttcctcac ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa
     8881 catcgggagg gcaagatttg tgacgagcat cacggaggat ttttttgcga tggcgcagaa
     8941 attgcgccat caacgatcgg tgataattac caaccacaaa catcatgttc gttttccgtg
     9001 tcttgacatc ctccacgccc tgaatgacga ggatccctgc tacgttcagg ctgtcgcctg
     9061 aatcatttcg gtgggttcct gcttcaacga gcggcctgac tgcaccatct ctccacaggc
     9121 aagaacggcg tgccccgccg ctaaaatgtt acgagcgccg tttacatcgg cgttcgctgt
     9181 atatccacat acctggcatc tgaattgact ttgtgacagg cgattttctt tcgctgtatg
     9241 accacagcac gcgcaacgct ggcttgtgta cgctggcgga acagcaagca cctgaccgcc
     9301 acgccagagc tgcttgtact caagctggcg gcgcatttca taccagccct gatccagtat
     9361 cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc
     9421 tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcattg cgtggttttt
     9481 gctgacggtc gttgtgactt tatgaaggta gtccctgcgg atatttgcga tacgggaatg
     9541 cagtcgctgt attttgcgtt tctgcttctg ccagttattg ctgaatttga ccttgcggct
     9601 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggccc
     9661 aaagactgtg ccatctgaca gcgtggcgag tttagccacg ccagcatcca gcccgatcat
     9721 tgatgctgaa gggtgaaccg gagttgatac ttcactttct gtctgaatac tgatgtacca
     9781 cttaccgcag gactggctga ccgtgacatt tttcacaaca cccgtgacct gacggctatt
     9841 ccggtagcgc atccagccca gtttcggcag aaaaatacgg ctgttttcct gatcgagctt
     9901 tacgccctgc gggtagcgga atgcatcatt ctgtccccgc tttttgaatc ggggaaaagc
     9961 agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg tctttcagtg actgttgcaa
    10021 tggctgtgag ggagaatctt taagccattg cgtttcagtg gcatttttcc actcaaccag
    10081 ccaggaagcc attttcccgt aagggatgta tttattaccg gcctcatgat tctcattctg
    10141 aagcgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcaca
    10201 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
    10261 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
    10321 cctgatgcat gttcacctgg tctttgtcac cagataccga ctccagattt ttgaccatga
    10381 cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg aagctgaact
    10441 tgttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc ctcccaaact
    10501 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga
    10561 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt
    10621 tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
    10681 aacacctggt taggtggaaa accgcgcctt atatccccgc cctgaaggac ggggttttac
    10741 ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca gccgccactt
    10801 ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg ccattgctgt
    10861 tattggtgac ggtcacggta tacgttgctt tgcccatgat tcatttcccg ttatgaatga
    10921 ctttccgttg ttgcgcacct tccatcagga cttcaggagc cacgaagaag tcaatgttga
    10981 aataagtatc gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg aacaccgcaa
    11041 actgccccgc ttcgataagg atcacctgat caggctctgc actgtgttca tcagcgtagc
    11101 cgagatattt gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc
    11161 ctttatcaag atgacgttcg aatattccgg caggcgcagt ttgtttattc cagaaaggcg
    11221 ttgagcgcgt atgaatataa ttctgtggga tttgcagcat cctattccct ccttcgggga
    11281 atgcgctgaa aacggtttat tccagccgtt tcagggtacg cctgataatt tgcattttaa
    11341 ataccattta ttggttactt tttagcacca tatcagagaa aaatcaggga ggattacaag
    11401 tgggaagccc gcgacaacgc gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg
    11461 gcgaaaccca cgaacatcaa accaatcagt gagttgccaa ctttagccag tttctttttg
    11521 gtacgtatgt actgcgtgac aaaagcacca gaaataatca ggaagctcaa atagcagaaa
    11581 ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa ttcccgtatg tggggcatta
    11641 acatcgataa actgtacgaa aaacgacaca tagaacaaaa tggctttcgg attagtcagg
    11701 ctcaaaatta acgcgcgttt aaaaatagca ccgtattggg gctcatcgga tttggcctcg
    11761 ttatttttac ccttcagggt cgcgtagaga attttactcc ccagatagag caaataaaac
    11821 gcaccaagat aacgtataat gttgaataat atcggggtgg tcttaattaa tgtcgccact
    11881 ccagcccatg ccagaaacat caataccgca tcgccaataa atacaccgca ggccgcaaga
    11941 taaccgcctt tcataccgct actgacgcta tttttgagta caaacagggt atttggccct
    12001 ggcaccaaca caataaaaat ggccccaacc agataggtcc agtaattcag aaccccgtat
    12061 tcagcgaaca cattaacctc tttaattatc tttcgatcat gcgcgattaa aggtgaatat
    12121 gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg ttttaacccg atatcaaccc
    12181 gataattgaa taattaatca ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa
    12241 tactggcgta aaaattctac cgttatccgc actttcgctg acgtcgccag ccttgaaaca
    12301 taaacggccc agacgttcgc tggctggtaa tattccggta gcacttgcac taaatgacca
    12361 ctggcaatgt tttcgctaac atcccaccag gaacgcagag caatcccctg cccatcgaga
    12421 caccactgat ggacaatttc tccatgatta gacgacagtg ggccggtgac tttaatcgca
    12481 tggtggcctt ctttattgcg taattgccag acaccaaaag gatgatcgcg ctctttaatg
    12541 accaggcacg gcaatgcaga aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata
    12601 aattccggcg acgcgcagag aatgcggtaa ttggtcgcca gtttgcgggc gattaaatta
    12661 ggggcgatgt catcgccaat gcgaatatcg agatcgacac cttcattgac caaatcgacc
    12721 agtcggtctt ccacatcaaa acgtaattcc agttgcggat acgccttcgc cagcgctgat
    12781 aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc
    12841 tggggcacct gacgcacgtc agaaagttcg tccatcatct gaccgacatc ctgcaaaatc
    12901 cgctgcgccc attcataaat ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg
    12961 cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac
    13021 gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac cagcccggcg agccaccagc
    13081 ataaagacgc gcaaatcgtt cagcagcggt aaattattca tgattcgtgt tttatgtttc
    13141 accagttacg gggattaatt ccttttcagt caattatagg atggtgatgt tgtcaatttt
    13201 gatggtcagg aagtgagaac ccaatgatga aaacgatgcg tattgctgcg atcccgggag
    13261 acgggattgg caaagaagtc cttcctgaag ggattcgcgt gttacaggct gccgctgagc
    13321 gctggggctt cgccttgagt tttgagcaaa tggagtgggc gagctgcgag tattacagcc
    13381 atcacggtaa aatgatgccg gacgactggc atgagcaact tagccgtttc gacgccatct
    13441 attttggtgc cgtcggctgg ccggacaccg ttcctgacca tatttcgttg tggggttcgc
    13501 tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc
    13561 ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga catcgatttt tacgtggtca
    13621 gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag agtgaatgaa ggtacagagc
    13681 atgaagtcgt cattcaggaa tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt
    13741 atgccttcga acttgcgcaa agccgcccac gtaagacgct aacttctgcc actaaatcaa
    13801 acggtttagc catcagcatg ccgtactggg atgagcgagt ggaagcaatg gccgagaatt
    13861 acccggagat ccgctgggac aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc
    13921 agccggaacg cttcgatgtg gtggtggcgt ccaatttatt tggcgatatc ctttccgatc
    13981 ttggcccggc ctgcaccggc accattggca ttgccccatc cgccaacctg aatccggaac
    14041 gcactttccc gtcgctcttc gagccagtcc acggttccgc gccggatatc tacgggaaaa
    14101 acattgctaa ccctatcgcc acgatttggg ccggtgcaat gatgctcgat tttctcggca
    14161 atggcgatga gcgtttccag caagcgcata acggtattct ggcagcaatt gaagaagtga
    14221 ttgctcacgg gccgaaaaca cctgatatga aaggcaatgc caccacgcca caggttgccg
    14281 acgcgatttg caaaattatt ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact
    14341 gacgcggttt ttttattcgt tctttgcagt aaataacctg cgtcatttca ccttttattg
    14401 tt
//
LOCUS       DAMZII010000119        14386 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZII010000119 DAMZII010000000
VERSION     DAMZII010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.119"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            46..612
                     /locus_tag="POA77_003635"
     CDS             46..612
                     /locus_tag="POA77_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6367003.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            663..3122
                     /locus_tag="POA77_003636"
     CDS             663..3122
                     /locus_tag="POA77_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6367004.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDN
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3137..3868
                     /locus_tag="POA77_003637"
     CDS             3137..3868
                     /locus_tag="POA77_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6367005.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVTLPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGRQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3865..4926
                     /locus_tag="POA77_003638"
     CDS             3865..4926
                     /locus_tag="POA77_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367006.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5078..6124
                     /locus_tag="POA77_003639"
     CDS             5078..6124
                     /locus_tag="POA77_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI6367007.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6121..6912)
                     /gene="nei"
                     /locus_tag="POA77_003640"
     CDS             complement(6121..6912)
                     /gene="nei"
                     /locus_tag="POA77_003640"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDI6367008.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTSEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6948..7682)
                     /gene="pxpA"
                     /locus_tag="POA77_003641"
     CDS             complement(6948..7682)
                     /gene="pxpA"
                     /locus_tag="POA77_003641"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6367009.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7672..8604)
                     /gene="pxpC"
                     /locus_tag="POA77_003642"
     CDS             complement(7672..8604)
                     /gene="pxpC"
                     /locus_tag="POA77_003642"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDI6367010.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8598..9254)
                     /gene="pxpB"
                     /locus_tag="POA77_003643"
     CDS             complement(8598..9254)
                     /gene="pxpB"
                     /locus_tag="POA77_003643"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI6367011.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9277..10020)
                     /gene="ybgI"
                     /locus_tag="POA77_003644"
     CDS             complement(9277..10020)
                     /gene="ybgI"
                     /locus_tag="POA77_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDI6367012.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10291..11772
                     /gene="dtpD"
                     /locus_tag="POA77_003645"
     CDS             10291..11772
                     /gene="dtpD"
                     /locus_tag="POA77_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDI6367013.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFRKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11814..13232)
                     /gene="phrB"
                     /locus_tag="POA77_003646"
     CDS             complement(11814..13232)
                     /gene="phrB"
                     /locus_tag="POA77_003646"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI6367014.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13229..13738)
                     /locus_tag="POA77_003647"
     CDS             complement(13229..13738)
                     /locus_tag="POA77_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI6367015.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13889..14386)
                     /locus_tag="POA77_003648"
                     /pseudo
     CDS             complement(13889..14386)
                     /locus_tag="POA77_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3463 a         3851 c         3496 g         3576 t
ORIGIN      
        1 tcttgcaccg atatactttt ttcttttctg ttaaaggact ccgttatgtt taaaggacaa
       61 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
      121 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
      181 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
      241 tcacatcaca gcgatgccgt tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac
      301 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
      361 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
      421 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
      481 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
      541 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
      601 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
      661 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
      721 cttgcgcatt ggcggtcgaa ttcaacctta atgttctcga taaatcaatg cgcgaccgca
      781 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
      841 ttgcggtaaa taataaccaa atcagtaacg ggcaaaagat taactggcac aaaaatgacg
      901 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
      961 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
     1021 tcttcaattt cgatcaagcc aatcagcaac taaatatcac cattccgcaa gcctggctgg
     1081 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc
     1141 tgatggatta caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta
     1201 atcttaacgc ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt
     1261 atcagttgaa tcagactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta
     1321 cctatctttt tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt
     1381 ttagttccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc
     1441 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
     1501 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag
     1561 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
     1621 cggaagaaga tggtcgggtg aacaatttcc aggtttcggc agcatcgacg cccttcctga
     1681 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
     1741 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
     1801 cctcgctgta cggcggcctg ctgctttctg gtgataacta ccattctgcc gcaataggta
     1861 tcgggcaaaa tatgctgtgg cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc
     1921 actttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
     1981 tggatgccac taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
     2041 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
     2101 agacgatcag cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca
     2161 acctgctaca tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag
     2221 gttttaatgt tgatattggt gactggagag atatctctat ttcgacgtca ttcaatacaa
     2281 cccattacga agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg
     2341 gtaacggtgg tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca
     2401 tgtcgtggaa cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat
     2461 ccgaccgtcc tgacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg
     2521 gtgagtggga tatttctggt acctatgccg ccaatgatta cagttccgtc agcagcagct
     2581 ggagcggttc tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca
     2641 atgaaccacg cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc
     2701 tcgactacac caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt
     2761 ccaccgtggc ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta
     2821 tcaaagaaac gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta
     2881 aagacgttaa cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata
     2941 tccgccagga tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt
     3001 taagcggagt cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct
     3061 cgctccatct tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt
     3121 aattaaggaa atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag
     3181 tctgtgcagc cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga
     3241 taaagctacc agtttgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc
     3301 ctggctcgaa aatgaaaaag gggagaaaag cgatgatctt ctggtcaccc tgccgccgat
     3361 acaacgtctg gaaccgaaag caacaacgca ggtacggata gtaaagcaag cctcgaccac
     3421 aaaattaccg ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc
     3481 cccggaaaaa aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt
     3541 gttctggcga ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca
     3601 ggtcagccag cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat
     3661 tgcctacctg ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc
     3721 gccttttagt acggtcaaca caaacaccgg aaattatagc ggcagacagt tttatctcgg
     3781 ttatatggat gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca
     3841 tttacaagcc gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt
     3901 ctgttatttc tgccgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt
     3961 ggctcggtag aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc
     4021 ggcgataaga tttgggaatc tgacgatatt aaaattcccg tctattgtga caacaatacc
     4081 aacggcaatt ttgaaagtga gcatgtttat gcctgggtaa acccctaccc gggggtgcag
     4141 gatcgctatt atcaactcgg cgtaacttac aatggcgttg attatgacgc caaccaggga
     4201 aaaagccgca tcgacactaa ccagtgtatt gacagtaaga acattgacat ttatacccct
     4261 gagcagatta tcgcgatggg atggcagaac aaaatttgct cgggtgatcc cgctaatatt
     4321 cacatgtcgc gtacctttct cgctcgcatg cggttatacg tcaaaatacg agaaatgccg
     4381 ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg tgcaatttga cggtgccggt
     4441 agcgttaacg aagatcctac tgcccaaaac ctgaaatatc atattaccgg tctggaaaac
     4501 attcgcgtgc tggattgcag cgtcaatttt tccatttcac cggaaacaca ggtgattgat
     4561 tttggtaaat ttaatttgct ggatattcgt cgccacacaa tgtcgaaaac attcagtatt
     4621 aagacgacaa aatcacaaaa tgaccaatgc accgacggat ttaaagtcag ttcctccttt
     4681 tataccgaag aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag
     4741 ctacgtttgc tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc
     4801 gatttcacca gcgacatgtt ggtctacgaa aaaacctata cggctgaact ttcgtccatt
     4861 gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat
     4921 aactgatttg ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg
     4981 attttcccct tttccttgat tgctgttatt ttttcctggc ccggctttct ttatgctgta
     5041 tcgatacacc aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat
     5101 gttatgtgtg ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc
     5161 gctattactt gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca
     5221 acttccccgc tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa
     5281 cctcactggt tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt
     5341 actggtgacg ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact
     5401 gcctggaaat accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat
     5461 ggcgcaagat tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct
     5521 gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg ctgggtgata acgctgaagc
     5581 ggttaaccag caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt
     5641 gctcgcagtc gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct
     5701 catcccgatg ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc
     5761 ggaatggctg ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga
     5821 taaacaaatc ttactgcggg cactacgccc gctaccgcaa atcctgctgt cgatttttgc
     5881 tctgctggct atttgtgcgg gtatggcgtg ggggctgacc cggtttatgc atattgattt
     5941 tatgactgcc tacctcgcca ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc
     6001 agggagcaat gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat
     6061 tttgctgacg gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc
     6121 ctagtgctgg cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat
     6181 gatgccgcca cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag
     6241 cgccccatga tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat
     6301 ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg
     6361 atttccagtc aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc
     6421 cagaaacgcc tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa
     6481 taaccgttcc tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg
     6541 ttgtaaaaac ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct
     6601 ataaagcaga atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg
     6661 cggctcttcg ctggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag
     6721 cgttaagtcg ttggaaaaat gagttaacaa cgccttacca cgcgtttcca catgggtaac
     6781 gtgttgaccg ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc
     6841 agttagtggt ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg
     6901 gccttcaggc atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca
     6961 acaatctcct tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg
     7021 ccgtcgccat gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt
     7081 ttgactctgc cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca
     7141 atcaacgcgc ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg
     7201 gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt
     7261 cctgccagcc caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg
     7321 tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga
     7381 cgcattacgc cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc
     7441 tgggcgtaaa cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg
     7501 ggaaaactcg ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc
     7561 tgcatggttt gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc
     7621 gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc
     7681 attgtgtagc cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag
     7741 tgcctcttcc agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag
     7801 atggtacata tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc
     7861 gtcgttcatc aacacaatag gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa
     7921 accgtgagat aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata
     7981 gcccatgcgg ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga
     8041 ggcgcgatcg aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag
     8101 cagctgttta acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg
     8161 gtcaccatcc ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct
     8221 gcatgacccc attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat
     8281 cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca atcgccagcc
     8341 ggtccagacg gcattatcat ccagccgcgc ttcgcaaccg gcccctgtca gagcaaacca
     8401 cccatcagtt tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt
     8461 ggcgtcatta cccaccagca ggttagcaat gcgcagcgcg ggcatatcca gtgcgccgca
     8521 gtggctgata cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat
     8581 gcccgcacga ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct
     8641 cccggacgta ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga
     8701 ccaatcaact gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg
     8761 atcccgacag aacctgccgg aacgagtaag cgcggttcag cgcgccgtgg cgtgtgtagt
     8821 tgttccggca aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg
     8881 tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg
     8941 accaccgcca aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga
     9001 gactcaggtt ccagggcctc gctctcctcc caccagcgtt gcaaacgctc tatggcatcc
     9061 agcgccagcg actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct
     9121 tcaaccacat tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta
     9181 gccagcgtca ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa
     9241 cgcgctcgtt gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat
     9301 aaaggtcaca tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc
     9361 acgttcagtg gcatggtgac ctgcagcata aaaatgcaac ccctgctcgc gggctgaatg
     9421 aatggtctgt tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct
     9481 atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc
     9541 ggtatcgcca caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc
     9601 cagtcccggc acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat
     9661 gaccgtgatc cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc
     9721 aagcggcaaa tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt
     9781 acgcttcatt ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat
     9841 gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc
     9901 ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc
     9961 actaatcgcc gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat
    10021 cttcgcgcct tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg
    10081 tcgatagcca atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt
    10141 ttatatcatc acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat
    10201 aatcagatct gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta
    10261 cgtgacgttg ccgtcaattg tggaattatt atgaataaac acgcatcaca accgcgcgct
    10321 atttactatg tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc
    10381 ctgctgattc tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta
    10441 tttagcgcct actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat
    10501 aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat
    10561 gtggtgctgg gtgccagtga gatccatccg tcattcctct atctgtccct ggcgattatc
    10621 gtttgtggct atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag
    10681 ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt atgcagcggg taacgtgggg
    10741 tctattatcg cacctatcgc ctgtggttac gcccaggaag agtacagctg ggcgatgggc
    10801 tttggcctgg cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt
    10861 catttcacac atacccgcgg cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg
    10921 cctaactggg gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtactg
    10981 ttcaggaaag agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta
    11041 ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt
    11101 gtgacgctca ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg
    11161 attagccttt atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact
    11221 gctatgttcc agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg
    11281 gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt
    11341 ggccttggcc tgatgagcgc cggattctgc attctgacct taagcgcccg ctggtccgca
    11401 atgtatggtc actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg
    11461 gaactgttta tcgacccggt tgccatgtcg caaattacgc gcattgaaat tccgggcgtg
    11521 accggcgtat taaccggcat ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg
    11581 ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac
    11641 tccatcaatg catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta
    11701 ggactggtac tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg
    11761 ctggaatctt aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct
    11821 cctttcctcg cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact
    11881 atcggttgcg gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc
    11941 accgctttcc ctggtacatc gcgcagctcc ggtaaccatc ggcggataaa ctcgccctca
    12001 cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg cgcagcatca
    12061 gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc accatcaatc
    12121 agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga
    12181 aaactggctg aaatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc
    12241 atggcggcat cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg
    12301 ggattgctct gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac
    12361 gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag
    12421 acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac
    12481 tggcgaggcg ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact
    12541 gccggaaaat ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat
    12601 tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga
    12661 taattcagcg taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca
    12721 gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta
    12781 aagactttgt acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg
    12841 tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc
    12901 cgctcattca cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac
    12961 acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga
    13021 ataccttttt ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc
    13081 tgacgcggcg acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc
    13141 acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg cagacgtaaa
    13201 tcctggcgaa accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca
    13261 cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca
    13321 tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt
    13381 cgataagcgc cgccaactgc tggcgttctg tggaatcaat atgcgggcga aaataaccct
    13441 gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa
    13501 ttacgcgttg gtagtactgg ttgtaaaaat catccagatt ttgccactgg tgaatatcag
    13561 ccacgaacgg gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat
    13621 agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta
    13681 actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgc tgattcatta
    13741 ctgctcctga acggtttaca ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt
    13801 aatcctgttg tagcaggaca aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg
    13861 tagtttctaa ccatagctgc cctttctatt caaacgtgaa atcaataagc tcatcaggga
    13921 catcgcaaac aatatgaaga cgggtttctt ctctgccgtg actcttttca ctaattgcgt
    13981 aactgtcatg cttgggatta tttaattctt tcagcggaaa tttttcctcg aaggctttat
    14041 ttagccgccc ctggtttcct tttacagcga ataaataatc acctccctgt ttttgtatct
    14101 tctctgcaat atctttctgg caacccatcg catcggttgt gatgattttt cctttaatat
    14161 ccaacatgtt aagaagttct gggatagctg tgatctcatt ggatttctta tccgtcttga
    14221 tctgcccgat gaccagactg tgcattgttg agaacgcact aatgacatga atcgctcccc
    14281 tgcggcgact cttgtcataa gagtgccgga gcgtttttcc atcaattgca atgacgtctt
    14341 tatcatttga tgaatggcaa tcacgcatcc agttaataaa gcactc
//
LOCUS       DAMZII010000120        14351 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZII010000120 DAMZII010000000
VERSION     DAMZII010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.120"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(106..858)
                     /gene="yecC"
                     /locus_tag="POA77_003649"
     CDS             complement(106..858)
                     /gene="yecC"
                     /locus_tag="POA77_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDI6367016.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MHPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="POA77_003650"
     CDS             complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="POA77_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDI6367017.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="POA77_003651"
     CDS             complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="POA77_003651"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDI6367018.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="POA77_003652"
     CDS             complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="POA77_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6367019.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="POA77_003653"
     CDS             complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="POA77_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDI6367020.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="POA77_003654"
     CDS             complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="POA77_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDI6367021.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5153..6985)
                     /locus_tag="POA77_003655"
     CDS             complement(5153..6985)
                     /locus_tag="POA77_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HDI6367022.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATTDNLTLAGFTAGTKAADGTVTYSKNVQFAAA
                     TASNVLAAAKDGDEITFAGNNGTGIAATGGTYTYHKDSNSYSFSATAASKDSLLSTLA
                     PNAGDTFTAKVTIGSKSQEVNVSKDGTITSSDGKALYLDEKGNLTQTGSGTTKAATWD
                     NLMANTDTTGKDAYGNSAAAAVGTVIEAKGMTITSAGGNAQVLKDAAYNAAYATSITT
                     GTPGDAGAAATAGNAAVGALGATAVDNTTADVADISISASQMASILQDKDFTLSDGSD
                     TYNVTSNAVTINGKAANIDDSGAITDQTSKVVNYFAHTNGSVTNDTGSTIYATEDGSL
                     TTDAATKAETTSDPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7251..8657
                     /gene="fliD"
                     /locus_tag="POA77_003656"
     CDS             7251..8657
                     /gene="fliD"
                     /locus_tag="POA77_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDI6367023.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNSIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8682..9092
                     /gene="fliS"
                     /locus_tag="POA77_003657"
     CDS             8682..9092
                     /gene="fliS"
                     /locus_tag="POA77_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDI6367024.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9092..9457
                     /gene="fliT"
                     /locus_tag="POA77_003658"
     CDS             9092..9457
                     /gene="fliT"
                     /locus_tag="POA77_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDI6367025.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9535..11022
                     /gene="amyA"
                     /locus_tag="POA77_003659"
     CDS             9535..11022
                     /gene="amyA"
                     /locus_tag="POA77_003659"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI6367026.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11056..11469)
                     /locus_tag="POA77_003660"
     CDS             complement(11056..11469)
                     /locus_tag="POA77_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6367027.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVECD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11656..12861
                     /gene="yedE"
                     /locus_tag="POA77_003661"
     CDS             11656..12861
                     /gene="yedE"
                     /locus_tag="POA77_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDI6367028.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12858..13091
                     /gene="yedF"
                     /locus_tag="POA77_003662"
     CDS             12858..13091
                     /gene="yedF"
                     /locus_tag="POA77_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDI6367029.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13198..13869
                     /locus_tag="POA77_003663"
     CDS             13198..13869
                     /locus_tag="POA77_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDI6367030.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(13909..>14351)
                     /locus_tag="POA77_003664"
     CDS             complement(13909..>14351)
                     /locus_tag="POA77_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6367031.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3428 a         3733 c         3610 g         3580 t
ORIGIN      
        1 tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac gcttgaaatg
       61 gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt gcagcagaaa
      121 cttctcgagg aactggcggg tgcgaggctg ctggggatca gcaaataacg cttttgctgc
      181 cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa catcccgggc
      241 aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca gctggcggat
      301 tgtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct cgtcaaacaa
      361 aatcacctca ggatgcattg ccagcgcacg cgcaatcgca acacgctgct gttgaccgcc
      421 agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg ccagcagctc
      481 gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat
      541 aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac
      601 gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat
      661 agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat ttatgctgcg
      721 tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt cgccaggctt
      781 tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca ccaggttctt
      841 aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt ctcaaaatgg
      901 ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc cgccaggtac
      961 atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa cagctccggc
     1021 acctggattg tcgcggccag cgacgtatct tttaccagac tgataaagct gttcgacagc
     1081 ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt ctgccacggc
     1141 gtcataccaa tactggcagc ggcttcccac tgacctttat caatagaaga aatggcggcg
     1201 cgcagcgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga
     1261 atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa cagttgtgcg
     1321 atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag
     1381 attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa aaacatgccg
     1441 ccgatactga gttgcagcgt atacccagcc cctttcaaca ggaacggcaa ggaatcaata
     1501 accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga tgataggcga
     1561 acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt
     1621 tctgactgat accgccaatc agccccgcca tcgcttttcc ggtatacaca ggatcaagca
     1681 gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc
     1741 cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca
     1801 gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg gcaacggaac
     1861 gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca
     1921 gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata ttaaccgccc
     1981 cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg ccaagagcat
     2041 tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca
     2101 gctcttccag ttgggcattg ggatcggtca gtgcatcgca catttcaatc tgggtattga
     2161 acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag
     2221 gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcgactgcg gcagtctggc
     2281 gcacatggtt agactggatc gcgccggcag taatcagcgt atcggcacct tcgcgcaggg
     2341 catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc atgggtgtga
     2401 cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt
     2461 cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa ttatgcagtg
     2521 gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt
     2581 aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac
     2641 atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt gcatttccgc
     2701 aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga
     2761 ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt tcttcaccag
     2821 atccagcgcc gccagacgat caacgaggat cgcatcgata cggcctacgc gcagatcctg
     2881 atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat tctgccgcag
     2941 ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca gatcagcagc
     3001 cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg aaatggtgta
     3061 aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct gattaatcac
     3121 cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag
     3181 tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt
     3241 taatttgccg tcatccccct gaaaactgaa cggcggataa gttccttcca gccctaccag
     3301 cagcgtgccg cgctctttga ctttattaag cagaccttca tctgcaaaac ttttaacgct
     3361 catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc ccagatgtgc
     3421 taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt
     3481 aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag
     3541 gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt gatacttccg
     3601 taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc
     3661 aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcgtgtagat gattccccaa
     3721 acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa
     3781 aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc
     3841 accgcaaaat cggcacaatg ccgcccatga ttgttgttcc acttgccagc cattttcatc
     3901 aagcagcgtg tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa
     3961 ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc
     4021 tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg
     4081 gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa
     4141 ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc
     4201 gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg
     4261 ctccggcaac gtttcgatag cttccatcac tcgctggcgc agattactgt ccagcagttg
     4321 ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat cgccgtgctc
     4381 ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg
     4441 ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc
     4501 aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct
     4561 gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac
     4621 tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa
     4681 cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg
     4741 caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc
     4801 cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt
     4861 atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag
     4921 gggttatttg ggggttaccg gtaaattcca tgcagaaaaa accccgccgg tggcggggga
     4981 gcacgttgct gacaaattgc gctttatgtt gccggatgcg gcgtaaacgc cttatccggc
     5041 ctacaaaaat gtgcaaattc aataaattgc aattcccctt gtaggcctga taagcgcagc
     5101 gcatcaggca atttggcgtt gccgtcagtc tcagttaatc aggttacgac gattaaccct
     5161 gcagcagaga cagaacctgc tgcggtacct ggttagcttt tgccagcacg gagttaccgg
     5221 cctgctggat gatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct
     5281 gaatacggga ctgcgcttcg gacaggttgg tagtggtgtt gttcaggttg gtgaccgcgg
     5341 aatccagacg gttttgcacc gcaccgaggg aggagcggaa tttgtcgatg gagctgatgg
     5401 cttcgtccag agctttcagg ggatcggagg tggtttcggc tttggttgct gcatcggtgg
     5461 tcaggctacc atcttctgtc gcataaatag tggagcctgt atcgttagtc acgctaccgt
     5521 tagtatgagc gaaataattg acaactttac tggtttggtc tgtgattgcg ccgctgtcat
     5581 caatgtttgc tgctttgcca ttgatagtga cagcattgct ggtcacgttg taagtatcac
     5641 taccatcact taaggtgaaa tctttatcct gaaggatgct cgccatttgc gaagctgaga
     5701 tagagatatc ggcaacatct gccgtggtat tatcaactgc cgttgcgccc agcgctccca
     5761 ccgcggcatt acccgcagtt gcagcggctc ccgcatcacc cggagtacca gtagtaattg
     5821 aggtcgcata tgcggcatta taagccgcgt cttttaacac ctgagcatta ccaccagcag
     5881 aagtgatggt cattcctttt gcttcgatta ctgtcccaac agctgctgcc gcagagttac
     5941 cataggcatc tttgcctgta gtatctgtat tggccatcag gttatcccag gttgcagctt
     6001 tggttgtgcc actacctgtt tgggtcaggt tgcccttctc atctaaatac agcgccttac
     6061 catcgctgga tgtaatcgta ccatctttgc taacgttaac ttcttgcgat ttagaaccaa
     6121 tagtcacttt agcggtaaat gtatcgccag cgtttggtgc cagtgtgctc aacagagaat
     6181 ctttagatgc agccgttgcg ctaaagctgt atgagttaga gtccttatga taagtataag
     6241 tccccccagt tgcagctata cctgtgccgt tattaccagc gaacgtaatt tcgtcgccat
     6301 ctttagcagc agccagtaca ttgcttgcag tcgcggcggc aaactggaca tttttgctat
     6361 aagttacggt gccatcagca gctttagtac ccgctgtaaa accagccaat gtcagattat
     6421 ctgtagtcgc agcggtattc gctacagaac ctttaccgtt aacgttaaaa ccagccagat
     6481 tcagcgtatc agagtcaatt ttcttcagat caatagtaat cgtttcgcca tcgttcgcgc
     6541 cgacctgaat tttcatcgag ccatctttag acagcacgtt cacgccgttg aactgggtct
     6601 gaccagatac gcggtcaatt tcgtcaagac gggatttgat ttcgtcctgg atggagtcca
     6661 ggtcagaggt ggagttagtt ccggtcgtcg cctgaaccgt cagttcacga atacgctgta
     6721 agttgttgtt aatttcggac agcgcgcctt cagtggtctg cgcaacagaa ataccgtcgt
     6781 tggcgttacg tgcagcctga gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg
     6841 cctgacctgc ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga
     6901 tagaactcga cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc
     6961 tgttggtatt aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg
     7021 tattgggttt ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac
     7081 aacctttaga atttttttca caaacagcca ttttttgtta gtcgccgaaa tactcttttc
     7141 tctgcccctt attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg
     7201 ccgataaccc cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta
     7261 tttcatcgct gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg
     7321 ccgcgcaaaa agcgacgcta acccctattt caaatcagca atcgtcgttt accgctaaac
     7381 ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat
     7441 tgtctaaagc cgatcttttt tccgccacca gcactaccag cagcaccacc gcgttcagtg
     7501 ccaccaccgc gggtaactcc atcgccggga aatacaccat cagcgtcacc catctggcgc
     7561 aggcgcaaac cctgaccacg cgcaccacca gagacgatac gaaaacggcg atcgccacca
     7621 gcgacagtaa actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca
     7681 gcgcggctaa ctcgtcgtta agcgggatcc gtgatgccat caacaatgca aaagcaggcg
     7741 taagcgcaag catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg
     7801 acaccggcct tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt
     7861 ttatgggcta tgacgccagt gccagcagca acggtatgga agtctcggtc gccgcccaga
     7921 atgcgcagct gacggtcaac aacgtcgcca ttgagaacag cagcaacacc atcagcgacg
     7981 cgctggaaaa catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca
     8041 tcactcagga cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctacaact
     8101 cgctaataga taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata
     8161 gccagagttc tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc
     8221 agttgaagtc gatgctgagt aataccgtta gttcttccaa ctataaaacg ttggcgcaga
     8281 ttggtatcac gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg
     8341 ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa
     8401 ccggcatcac gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta
     8461 ttaaagccgc taccgatggc gttagtaaga ccctgaataa attaactaaa gactacaacg
     8521 ccgccagcga tcgcattgat gcgcaggtcg cgcgctataa agaacaattt acccaactgg
     8581 acgttttaat gacctcgttg aacagcacca gcagctactt aacgcagcag ttcgaaaaca
     8641 acagtaattc caagtaatca atattcatcg ggagacaggt catgtacgcg gcaaaaggca
     8701 cccaggccta tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc
     8761 tggtcaccat gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc
     8821 aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga
     8881 acggactgcg ggtgagtctt gatgaagaga gcaaagacga attaacccaa aacttgattg
     8941 ctctttatag ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac gatgtctccg
     9001 cagtcgaaga agtggaagca ttaatgcgaa atattgccga tgcctggaaa gagtcgttac
     9061 tctccccttc tttgattcag gacccagtct gatgaaccac gcaccgcatt tgtatttcgc
     9121 ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg
     9181 ggacgaactc atcgccagcg aaatggcgta tgtgaatgcg gtgcaggaga ttgcacatct
     9241 gactgaagag attgacccgt ccaccacgat gcaggagcag ctccgcccga tgctgcgcct
     9301 gattctcgac aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc
     9361 gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg
     9421 cggctttgtg ctggctccgc aagataactt ctcgtgaatc ttaacaagct aatggctcgc
     9481 gaataatctg attacggcta cgcttctaat gttccccttg aatggagtcg aagaatgcgt
     9541 aatcccacgc tgttacaatg ttttcactgg tattacccgg aaggcggtaa gctctggcct
     9601 gaactggccg agcgcgccga cggttttaat gatattggta tcaatatggt ctggttgccg
     9661 cccgcctata aaggcgcatc gggcgggtat tcggtcggct acgactccta tgatttattt
     9721 gatttaggcg agtttgatca gaaaggcagc atccccacta aatatggcga taaagcacaa
     9781 ctgctggccg ccattgatgc tctgaaacgt aatgacattg cggtgctgtt ggacgtggta
     9841 gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct
     9901 gatgaccgta cgcaaattga cgaagaaatc attgagtgtg aaggctggac gcgttacacc
     9961 ttccccgcgc gtgccgggca atactcgaag tttatctggg atttcaaatg ttttagcggt
    10021 atcgaccata tcgaaaaccc tgatgaagat ggcatattta aaattgttaa cgactacacc
    10081 ggcgaaggct ggaacgatca ggttgatgat gaattaggta atttcgatta tctgatgggc
    10141 gagaatatcg attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg
    10201 gtgatggaac aaacgcaatg cgacggtttt cgtcttgatg cggtcaaaca tattccggcg
    10261 tggttttata aagagtggat cgaacacgta caggaagttg cgccaaagcc gctgtttatt
    10321 gtggcggaat actggtcgca tgaagttgat aagctgcaaa cgtatattga tcaggtggag
    10381 ggcaaaacca tgctgtttga tgcaccgctg cagatgaaat tccatgaagc atcgcgcatg
    10441 gggcgcgact acgacatgac gcagattttc acgggtacat tagtggaagc cgatcctttc
    10501 cacgccgtga cgctcgttgc caatcacgac acccaaccgt tgcaagccct cgaagcgccg
    10561 gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt tgttgcggga aaatggcgtt
    10621 ccttcggtat tctatccgga cctctacggt gcgcattacg aagatgtcgg cggtgacggg
    10681 caaacctatc cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt
    10741 cagcgtttcg cccacggtgt acagacgtta tttttcgacc atccgaactg cattgccttt
    10801 agccgcagtg gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa cggggatgat
    10861 ggcgaaaaaa ccattcatct gggagagaat tacggcaata aaacctggcg tgattttctg
    10921 gggaaccggc aagagagcgt agtgaccgac gaaaacggtg aagcgacctt cttttgcaac
    10981 ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt aaattcatcc ccggcagcaa
    11041 gccggggaga tttcattacg gcagtggcgt cggtaacggc gctttatcca gcgcggcggc
    11101 acactctgct gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt
    11161 accgtcacac tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga
    11221 catcaatgtc aacttacccg gtacggcaat cacccgctgc cactgacggc aatcgagggt
    11281 atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc ggactgacca gcgcacgttg
    11341 cggccctttg gtctgccagt agcccgccag ccagtccggc gcgggggttt tcacgacatt
    11401 gttatagttt tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg caattgcgag
    11461 ttttttcatc attcgtcctg catgagaaga aaagaggatt gtggcattaa agccctgatg
    11521 tcgccagcct ttctaggttc agagagaaat ttgatctaac tattattttt gtgaattatt
    11581 accccgaaaa taaatttgct tgatgtatga ttcgcgattc ttgtttcaaa ccttctgagt
    11641 tcagaggcta cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg
    11701 gcacccatcc ctgcggtcat cgcggcgggt attctctcca cttactattt tggcattact
    11761 ggcacctttt gggctgtcac gggtgaattt acccgttggg gcggtcagct cctgcagctg
    11821 tttggcgtcc atgctgaaga gtggggttat tttaaaatta tccatctgga aggatcgcca
    11881 ttaacccgca tcgacgggat gatgatcctc ggtatgtttg gcggctgctt tgctgcagcg
    11941 ctgtgggcca acaatgtcaa actacgcatg ccgcgcagcc gtatccgcat tatgcaggcc
    12001 attattggcg gcattatcgc cggttttggc gcgcgtctgg caatgggctg taacctggcg
    12061 gcgttcttta ccggcattcc tcagttctcg ctgcatgcct ggttctttgc catcgccact
    12121 gccattggtt catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg
    12181 aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt
    12241 cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg gctgggcgct gctcacggcg
    12301 atgaaccaac ccaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt
    12361 gaacgtgcgc aaatctgctt tacttcagcg ttccgcgata tgtggatcac cggacgtacc
    12421 catatggcga aagcaatcat tattggtatg gcggtgagtg ccatcgggat cttcagttac
    12481 gtacagttag gcgttgaacc caaaatcatg tgggcgggac caaacgcggt aattggtggt
    12541 ttactgtttg gttttggcat cgtgctggca ggcggctgcg aaaccggctg gatgtaccgc
    12601 gcggtagaag gccaggtgca ctactggtgg gtcggtctgg gcaacgtgat cggctcaacg
    12661 attctggcgt attactggga tgattttgct ccggcgctgg ccaccgactg ggacaaaatc
    12721 aacctgctga aaacctttgg cccgatgggt ggcctgctgg tgacatattt gctgttgttt
    12781 actgcgctga tgttgattat cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag
    12841 actgctaagg agatcgcatg aaaaatatcg ttcctgatta ccgccttgat atggtgggtg
    12901 aaccctgccc ttatccggca gtcgcaactc ttgaggcgat gccgcagtta aaaaaagggg
    12961 aaatcctgga agtggtgagc gactgtccac agtcgatcaa taatattcca ctggatgcgc
    13021 gtaatcacgg ctatacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa
    13081 ttcagaagta atccctcatt cccgctgtgg cccacagcgg gaacatcgtt gcctctgaca
    13141 catagatttt acccgccccc tctcctattc ttttcagtat gatctcagga ggtttctatg
    13201 tgtggacgct ttgcccaatc ccaaacgcgt gaagattacc ttgcgcttct cgcggaagat
    13261 attgaacgcg atattcccta cgatcccgaa cccattggca gatacaacgt cgcgccggga
    13321 accaaagttc ttctgctcag tgaacgtgat gaacaccttc atctggatcc ggttttctgg
    13381 ggatatgctc ccggatggtg ggataaaccg ccgctgatta acgcccgcgt agaaactgcg
    13441 gccaccagtc gtatgtttaa accgctctgg caacatggtc gggcaatctg ttttgccgat
    13501 ggctggtttg agtggaaaaa agaaggcgac aaaaaacagc cttattttat ctatcgcgct
    13561 gatggacaac ctgtttttat ggccgcgata ggtagcacac cgtttgagcg tggtgacgaa
    13621 gccgaaggat ttttaattgt cactgcagca gcagaccagg gtctggtaga tattcatgac
    13681 cgccgcccgc tggttctgtc gccagaagct gcacaggaat ggatgcggca agagatcggc
    13741 ggtaaagaag catcagaaat tgctaccaac ggctgtgtgc cagcaaacca gttcacctgg
    13801 cacccggtat cgcgcgcggt aggtaatgtc aaaaaccagg gcgcggagtt aattcaacct
    13861 gtttgttgac atcctccacg ccctgaagga cgaggatccc tgctacgttc aggctgtcgc
    13921 ctgaatcatt tcggtgggtt cctgcttcaa cgggcggcct gactgcacca tctctccaca
    13981 ggcaagaacg gcgtgccccg cagctaaaat gttacgagcg ccatttacat cggcgttcgc
    14041 tgtatatcca catacctggc atctgaattt actttgtgac aggcggtttt ctttcgctgt
    14101 atgaccacag cacgcgcaac gctggcttgt gtacgctggc ggaacagcaa gcacctgacc
    14161 gccacgccag agctgcttgt actcaagctg gcggcgcatt tcataccagc cctgatccag
    14221 tatcgaacgg tttaaacctg attttgcccg gacattgcgc cccggctggc tgaccgtacc
    14281 cgctgctgac tttgacatgt tgctgacctt caaatcctca atgactatca ttgcgtggtt
    14341 tttgctgacg g
//
LOCUS       DAMZII010000121        14292 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZII010000121 DAMZII010000000
VERSION     DAMZII010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.121"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(128..208)
                     /gene="shoB"
                     /locus_tag="POA77_003665"
     CDS             complement(128..208)
                     /gene="shoB"
                     /locus_tag="POA77_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDI6367032.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(628..1008)
                     /locus_tag="POA77_003666"
     CDS             complement(628..1008)
                     /locus_tag="POA77_003666"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI6367033.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(1008..1739)
                     /gene="pdxJ"
                     /locus_tag="POA77_003667"
     CDS             complement(1008..1739)
                     /gene="pdxJ"
                     /locus_tag="POA77_003667"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDI6367034.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1751..2479)
                     /gene="recO"
                     /locus_tag="POA77_003668"
     CDS             complement(1751..2479)
                     /gene="recO"
                     /locus_tag="POA77_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI6367035.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2491..3396)
                     /gene="era"
                     /locus_tag="POA77_003669"
     CDS             complement(2491..3396)
                     /gene="era"
                     /locus_tag="POA77_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI6367036.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3393..4073)
                     /gene="rnc"
                     /locus_tag="POA77_003670"
     CDS             complement(3393..4073)
                     /gene="rnc"
                     /locus_tag="POA77_003670"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6367037.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4346..5320)
                     /gene="lepB"
                     /locus_tag="POA77_003671"
     CDS             complement(4346..5320)
                     /gene="lepB"
                     /locus_tag="POA77_003671"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6367038.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5336..7135)
                     /gene="lepA"
                     /locus_tag="POA77_003672"
     CDS             complement(5336..7135)
                     /gene="lepA"
                     /locus_tag="POA77_003672"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI6367039.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7333..7812)
                     /gene="rseC"
                     /locus_tag="POA77_003673"
     CDS             complement(7333..7812)
                     /gene="rseC"
                     /locus_tag="POA77_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDI6367040.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7809..8765)
                     /gene="rseB"
                     /locus_tag="POA77_003674"
     CDS             complement(7809..8765)
                     /gene="rseB"
                     /locus_tag="POA77_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDI6367041.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8765..9415)
                     /gene="rseA"
                     /locus_tag="POA77_003675"
     CDS             complement(8765..9415)
                     /gene="rseA"
                     /locus_tag="POA77_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDI6367042.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9448..10023)
                     /gene="rpoE"
                     /locus_tag="POA77_003676"
     CDS             complement(9448..10023)
                     /gene="rpoE"
                     /locus_tag="POA77_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDI6367043.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10020..10175)
                     /gene="rseD"
                     /locus_tag="POA77_003677"
     CDS             complement(10020..10175)
                     /gene="rseD"
                     /locus_tag="POA77_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDI6367044.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10431..12053
                     /gene="nadB"
                     /locus_tag="POA77_003678"
     CDS             10431..12053
                     /gene="nadB"
                     /locus_tag="POA77_003678"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDI6367045.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPIPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12038..12775)
                     /gene="trmN"
                     /locus_tag="POA77_003679"
     CDS             complement(12038..12775)
                     /gene="trmN"
                     /locus_tag="POA77_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDI6367046.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12907..14241
                     /gene="srmB"
                     /locus_tag="POA77_003680"
     CDS             12907..14241
                     /gene="srmB"
                     /locus_tag="POA77_003680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDI6367047.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3443 a         3820 c         3558 g         3471 t
ORIGIN      
        1 aattgatgac attagcataa tcattcacta agttaattta tatagtatct gccaagacac
       61 ttatttatag ttattaaagg cgcgtccgat tggttcaccg gacgcacctt aagtacgttt
      121 ccttgtgtta taagaacaga aggatcagct gtaaaacagc aatgatgatt ttgatgaccc
      181 gtttaatcag gtatcggcaa tcagtcattc gtttttcctt aaacaaggaa tgcagccgtt
      241 cagattagcc cttacatctc cccaaaactg aacgtgcgag ttattgaggg tgcatgctgc
      301 actccacacc agagctttga cgacaccact cgtttcaatg ggggaattct gtggcatggt
      361 gtaaagcaca gcaaaatctt caataacgaa gccaatttta atgtacttac gaattggcgg
      421 tcacgttaat ctttccatca atattgcttt cttcgtaaag gctcgagttt ttatgctaaa
      481 gattgcaagt tgcttgtaaa agataagtac actgatccat aatcgctgtt gttgagggtg
      541 catgctgcac aaaattaaag ttaaaaagta aaacccccgt tccttaccag ttcgggggtt
      601 ttacttttta aagagaacgg tattatttta actttcaata attaccgtgg cacaagcata
      661 gtggcgttca tctgccagcg ttacatgcat atttacaacg ccgagctttt ccgccagttt
      721 taatgcctca ccccatagcc gtagccgtgg tttgccgagc tcatcattga atacttcaaa
      781 ttgattaaac gccagaccat tgcggatccc ggtgccaaac gcttttgctg cggcttcttt
      841 cacagcaaaa cgcttcgcca gaaaacgcac cggctggtgg tgcgttttcc agattgccca
      901 ttcgttatcg cttaatacgc ggcgtgccag gcgctcaccg gatcgggcga tcactgcttc
      961 tatgcgagcg atctctacga tatccgtgcc taaacctaat attgccatta gccacgcgct
     1021 tccagcatca gacgcttcat ttctgccacc gcatctttca gtccggtcat cactgcacga
     1081 ccaataatgg catgaccgat attcagttca tgcatctcag ggatggcggc aatagctttc
     1141 acgttgtgat aggtcagacc gtgaccagcg ttaactttca gaccgagact agcggcaaag
     1201 gtcgcggctt tggcgatacg cgccagctct tgcgcctgtt cggcgtcagt tttggcatca
     1261 gcatagcaac cggtgtggat ctcgataaac ggtgcgccaa cctctgccgc agctttgatc
     1321 tgctcttcat cggcgtcgat aaagagagaa acctggatcc cggcatctgc cagacgtttg
     1381 caggcatcgc gcattttgtc acgctgccct gcgacgtcca ggccgccttc ggttgttact
     1441 tcctgacgct tttccggtac cagacagcaa aaatgtggct tcgtctcaac ggcgatcgcc
     1501 agcatctctt cggtcaccgc catctccaga ttcatgcggg tatccagcgt ctgacgcagg
     1561 atgcgcacat cacggtcagt aatgtggcgg cgatcttcac gtaaatgcac ggtaatgccg
     1621 tccgctcccg cctgctcagc aataaacgcc gcctgcaccg gatccgggta agcagtaccg
     1681 cgtgcgttac gcagcgttgc aatatggtca atgttgacgc ctaacagtaa ttcagccatg
     1741 acaatcctca tcattcataa tgtgttttca ccgttcgctt aggcataaac tgccggaaca
     1801 gttccctgct ctttaatggt ttaccgccaa gatacggctt aagcgccatg cgggtaaagc
     1861 gtttcgcggc gcgtagtgtg tctgcgtcag gaaattcccg cgcgtttaac gcttttaact
     1921 gccttccggt gaacgtttta ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc
     1981 gataacgata cgtcatggtg tcatctaccg gctcgccgct acccgcacaa tgggtaaaat
     2041 tgacgccata gcccagatgc ccgagcagtg ccagttcaaa gcggcgcagc gcgggttctg
     2101 gcgtaccagt ggcacctgca agagactgaa tgcagtgcaa gtaatcgaaa aagagttcag
     2161 agaagcgcgt ctcgtattcc agtacgcggg agagaagttc gttgatgtac agaccgctgt
     2221 aaagcgtgat accgcttaat ggcagcgcca gcgagacagc ttcagcactg cgcagcgttt
     2281 tgacttcgcc acgcccgccg aaacgtagca agagaggggt gaaaggctgt aatgcacctt
     2341 tcagagtaga gcgtttagag cgtgcgcctt tggcaaccag acgcacgcga cccgattcct
     2401 ccgtgaagac gtccagcatc aggctggttt cgctccacgg gcgactatgc aggacaaatg
     2461 cgcgctgcca gccttccatc ggagtgactc ttaaagatcg tcaacgtaac cgagactgcg
     2521 cagtgcgcgt tcgtcgtcgg cccaaccgga tttcactttt acccacagtt caaggtggac
     2581 aggcgcttcg aacatttcct gcatgtcttt acgcgcttca atcccgatgg ttttgatctt
     2641 ggcccctttg ttgccaataa ccatcttctt ctgcccttca cgctcaacga gaatcaaacc
     2701 attgatgtca taaccaccgc gttcgttaga gacgaaacgt tcgatctcca cggtcacgga
     2761 gtacggcagt tcggcgccga ggaaacgcat cagtttttcg cggatgattt cagacgccat
     2821 aaaacgctgt gagcgatcgg tgatgtaatc ttccgggaag tgatgggtcg cttcagggag
     2881 atgcttacgc acgattgctg cgatagtgtc gacattcagc ccggtttcag cagagatcgg
     2941 cacgatatcg aggaagttca tctgacttgc caggaactgt aagtgcggca gcagatcggc
     3001 tttttcctgc acgttatcca ctttgttcac cgcgaggatc accggcgctt tgccatcgcg
     3061 cagtttgttg agcaccattt cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat
     3121 caccagctca acgtcgccaa tagagctgct cgccgctttg ttcatcaggc ggttaatagc
     3181 gcgtttttct tccatatgca ggccaggggt atcaacgtag atcgcctgat acgcgccttc
     3241 agtatggatc cccacaatgc ggtgacgagt tgtttgcgct ttgcgggagg tgatggagat
     3301 cttctgcccc agcagtttgt tcaacaatgt ggatttgcca acgttcggac gtccgacgat
     3361 ggcaataaat ccgcagtaac ttttatcgat gctcattcca gctccagttt tttcaacgcc
     3421 tgttcggcgg cagcctgctc agccttacga cggcttgaac ctgtgccaac caccggttca
     3481 ctcaggccgc tgacctggca gtggatagta aattcctgat cgtgcgcttc gccacgtacc
     3541 tggactacca gataagtcgg cagcggcaga tggcgaccct gcaaatattc ttgcaagcgc
     3601 gttttcggat ctttttgttt atcgcctggg ctaatttcgt ccaaacgagt ttgataccag
     3661 ttgaggatta atttctcgac ggtttgaata tcactgtcga ggaatacgcc accaattaat
     3721 gcttcgacgg tgtcggcgag aattgactca cgacgaaatc caccgctttt aagttcacct
     3781 ggccctaaac gtaagcactc gcctaactca aattcgcgcg ccagttccgc cagcgtattg
     3841 ccacggacca gcgtggcgcg catccggctc atatcgcctt catccacacg agggaaacgg
     3901 tgataaagcg cattggcgat aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc
     3961 tcgttatgtt tgctgctggc actacgatga gttaatgcct gctgcaacag ttcctgatga
     4021 ttaaaagtgt agcccagctt ccgttgaagc cgattaatta cgatggggtt catgcgatac
     4081 caataaataa atgcgtcaac aattcagcac acgaaacaga cctgatatac atggctctgc
     4141 taactgcttc gctgcagttt cagtttaccg gtatatgggg accaacgctg tttcgtgtgc
     4201 cgtggcaacc tggaggtgcc aaccttaaac ttcgggggaa tattctatac acaacgacgg
     4261 gggatgtcgt tagccacggg agatttatct cataaataat tcacgttgtc gccataacgg
     4321 cgacaacgtg aacgaagatg ggctattaat ggatgccgcc aatgcgactt aagcgcacac
     4381 ctgtcggcca ttcgccttct tgcttatcga aactcatcca gatagccgtt gcccgaccga
     4441 ccagattcgc ttcaggcaca aaaccccagt aacggctgtc cgcgctgttg tcgcggttgt
     4501 cgcccatcat gaagtattgt cccggcggaa caatccaggt tgccagttgt tgccctggct
     4561 gctggtaata catccccacc tggtcctgcg caatcggcac tgtcagaatt cggtgcgtca
     4621 catcacccag tgtctcttta cgctcggaaa gacgaattcc attttctttg gtttcatttt
     4681 tcggcacttc aaagaatccg ctggtcgctt ccccaccatt acggcgtgag aaggtctgaa
     4741 cgaaatcgct cggttccacg ttggagtagg tgaccggtag cgcgttttca cacgcctggc
     4801 cggaactgca tcccggttga atcgtcagct cttttgagac cggatcgtaa gtgactttat
     4861 cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt tggatcttcc ggatatttaa
     4921 agaccacgat atctccgcgt ttcggatgac cggtttcgat cagcgttttc tggtagatag
     4981 gatctttaat gccataagca aacttttcta ccagaataaa atcaccgatt aacagagtcg
     5041 gcatcatcga acctgacggg atctggaacg gttcataaat aaacgaacgc acaatcaata
     5101 cgatagccag taccggaaaa acagaagcac cggtttccag ccagccaggc ttcggcgcaa
     5161 cctttttcaa cgttgcttta tccagtgagt caccggcagc cgcctgcgcc gctgcctgac
     5221 gttcccgccg tttaggtgcg aaaaagaatt tatccacgca ccataaaatg cccgtcacca
     5281 gtgtggcaat caccagaatc agggcaaaca tattcgccat gccaactcct aagggttatt
     5341 tgttgtcttt gccgacgtgc agaatggcga ggaacgcttc ctgcggcagc tcgacgttac
     5401 cgatctgctt catgcgtttc ttaccttctt tctgcttctg cagcagcttt ttcttacggc
     5461 tgatatcgcc gccataacat ttagccagta cgtttttacg cagctgtttc acggtggatc
     5521 gcgcaataat gtgcgtacca atcgctgcct gaatggcgat atcaaactgc tggcgtggga
     5581 tcagatcttt catcttctcc accaactcgc gaccgcggtt ttgcgaatta tcacggtggg
     5641 tgatcagcgc caacgcatca acacgttcac cgttgattaa tacgtctaca cgtaccatgt
     5701 cggacgcctg gaagcgtttg aagttgtaat ccagagacgc ataaccacgc gaggtagatt
     5761 tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg gatctcgtac gtcagcgcca
     5821 cctgattacc gtggtaaacc atattggtct gcacgccgcg tttttctacg cacagcgtaa
     5881 taacgttgcc gagatatgcc tgcggcagca gcatgtgaca ttcggcaatc ggctcgcgca
     5941 gttcgtagat gttatttacc gctggcagct tggatggact gtcgacgtaa ataacttctc
     6001 ttgacgtggt ttcaacttca tacactacgg tcggcgcagt ggtgatcaga tccagatcgt
     6061 attcacgttc cagacgttcc tggatgatct ccatgtgcag caggccgagg aagccgcagc
     6121 ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata gaacagtgaa gcatcgttca
     6181 ggctgagttt acccagcgcg tcacggaagg cttcatagtc gtcggaactt accgggaaca
     6241 gaccggcgta tacctgcggt ttgactttct taaagccagg cagcgccttt tctgccggat
     6301 tacgcgccag cgttaaggta tcgccgactg gagcgccgtg gatatcttta atcgcacata
     6361 ccagccagcc tacttcgcca catttcagtt cagtgcggtc aacctgtttc ggcgtgaaga
     6421 tgcccagacg gtcggcgtta taggtctgcc cggtactcat gactttcact ttgtcgccct
     6481 tacgcagggt gccgttttta atacggataa gtgaaacaac gcccaggtag ttgtcgaacc
     6541 atgagtcgat aattagtgcc tgcaacgggc cttccggatc gccttccggc ggcggaatgt
     6601 cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc ggttttcgct gaacagcgca
     6661 ccgcgtcagt ggcgtcgatg ccgacgatat cttcaatttc ttccgccacg cgttcaggat
     6721 cggctgccgg caggtcaatc ttgttcagta ctggcaccac ttcgagatcc atttccatgg
     6781 cggtgtagca gtttgccagg gtttgcgctt ccacgccctg cccggcgtcg accaccagca
     6841 atgcaccttc acaggcagcc agcgaacggg aaacttcata ggagaagtct acgtggcccg
     6901 gggtgtcgat aaagttaagc tgataggttt cgccgtcaga cgctttgtag tccagcgtca
     6961 cgctttgcgc tttgatggta atgccacgct cacgctcaag atccatggaa tcgagaacct
     7021 gcgcctccat ttcacggtca gacaggccac cgcagatctg gataatacgg tcagacagcg
     7081 tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt acgtatattc ttcataaagt
     7141 atgattattg tgccttaatg cccggttaac caggctttta gaagtcgctg ttctgagctt
     7201 aacgtctgta ttaatagaaa cgccgcattc tacactacaa cattgaggcg aggaaatgtt
     7261 cataccgtat ggattgtggt atctggaaac gtcctcgcat ttgttatgca aaatgcaaca
     7321 aagccagtga aatcactggc tcgcgtcttc cgaagatgtt tcaaatcgca caaggccagg
     7381 cggcaatgcc acgcttaaga tgatcggttg ccattctgcc cgggcagcaa acttacgcga
     7441 gtagccgcgc gcaatcagga acccgccaat accaccgagg atcgcaccac ataatgctgc
     7501 gacgtcagaa gcaaagagta gctgaaatag cgaagcgata aggaataatc ccaccagcgg
     7561 cgacatataa accagtaatg cggagctaag caggctgcct tcggcgatcc ccaattccac
     7621 tttttgcccc ggcactaacg gttcatcaca gggtacgaca atggtatgcg tggtttgcgg
     7681 gccaagttta ttcaacacgc ggctaccgca accggcgcgt gaagcgcagc tgctgcatga
     7741 agctttaaca tcacaactga ccagcgcctg cccgttttgc caggagacga cggtagccca
     7801 ctctttgatc attgcgctgc cccgaactta atattctcgg caatgcgttt cgccgtttgc
     7861 ggcggcagtt caccgacaat ggtgatttcg gcgttatcac gtacgcttgt actgacggtt
     7921 ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg tagcgcggtt aacgtttacc
     7981 gagaagctga ataatccgtc ggaatagaga cgtgattcga taggcatgtt gtccatcgtc
     8041 ggtagcggac gtcgactact ggaaacttcg ctaaaaccct gtggcaacca ggttggcgtc
     8101 cagctgaatt tagctttttc acctacagga acagaaagca acggcggcaa atttgccttc
     8161 gccagcgtct gcatactgct actgatatcc tgattgacgt taaaagcaat cacgcgaaat
     8221 tgttccagcg tttcaccatc gcgatcaagg agatcaaccc tcatcggtaa tttcgattcg
     8281 atgtccatcc acacgatgta gctgtagcgt gtaccatctc gggcaaccac gcgaatgact
     8341 tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga taaagtcgta gtaaggagaa
     8401 aggcgtttga aatcggtata gatgagcgat ggcagagaat caacaatgta atcgccatta
     8461 agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt cattgccacg ctgtaccact
     8521 tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag gacggttatc gaggcgtgca
     8581 tgtcgataac gcagagactc aacaccctgt ttattgatgc tgatgaatga cagctcgtaa
     8641 ttcagtgact gactggccag gttcatctgc tgtaataacg ccccggacgc gggagtggcc
     8701 gaggcgttag cagagaataa caggctacct gtcactaatg acatggcaaa ccaaagttgc
     8761 ttcattactg cgattgcgtt cctaaagttt gaattcctgg cacctgtaca gcggcttgct
     8821 gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag tcggcgttgc agttcgtaat
     8881 cctgcaacat tgcattaatg cgacgacgct gctcctgtac ctgctgctgt tgaccattgt
     8941 ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct ggctttaccc atcatcggca
     9001 gtgtattaaa taccggcgtt tcgggctgct gggacgtttc agattgtcca ttatagtgct
     9061 ggacgccaac gataactgca agcgatacgc atgctgctac gcccatttgg gtaagctgtg
     9121 ccgcccacgg acgtactttc tgccagaatg gcattttctg ccattgatgc ggcgcaggct
     9181 gggcttccgg gatcaatgtc gccggttgac gtactggctc ttcttcaatg gcggccatca
     9241 cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc accccgcatt gagtcacgga
     9301 ttaagtgata gctttcccag gttttctgca tttctgggtt atgagccagt tcgttaagca
     9361 gctcactatc cagcgtttcg ccatccatta aagcggaaag ttgttctttc tgcatgccta
     9421 atacccttat ccagtatccc gctatcgtca acgcctgata agcggttgaa ctttgttatc
     9481 aatagcttcc ctcgctcgga agatacgtga acgcaccgta cctaccggac aatccatgat
     9541 agcggctatc tcttcatagc tcaggccatc cagctcccgc aaggttattg ccatgcgcaa
     9601 atcttccggg agggactcaa tagttcggaa aactatctgt ctcagttctt ctgacaacat
     9661 taagttctca gggttcgaaa tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc
     9721 aatggcatcc acatcactgg aaggtggacg acgcccctga gcaaccaggt aatttttcgc
     9781 tgtatttaca gcaatccgat acagccatgt ataaaaagcg ctatctcccc ggaacgaatc
     9841 cagcgcacga taggctttaa taaaagcttc ttgtaccaca tcgggaacat cacccgacgg
     9901 cacatagcgg gaaaccagac tcgccacttt atgctgatag cgcactacca gtaggttaaa
     9961 ggctttctga tctcccttct ggacccgttc aaccaggacc tggtccgtta actgctcgct
    10021 catccgaggt aaagtctccc caaaccaaat ttccacgcgc tatcgaaacg ccactccatt
    10081 agctgcaatt tgagcaagca aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc
    10141 atctgttttt gtttgtcatg ctgtagacgg atcattatct atcattataa gtctacagaa
    10201 tctgaacatc gcattatctg tgtagaaatg cccatttaac tgcctgaaga gtaacccaac
    10261 ggccttttta tttcaccacc taatcctcca ccagccagta acttctcttt ttctcgccgc
    10321 cctgcgtcag cgtgtttagc cactgtaaca aatattaaaa tagcaggtgt ttattcgcac
    10381 aacatgatgc tatgctgacc aaacggtgtt tagtaaatta aacaaagaaa atgaatactc
    10441 tcccagaaca ttcatgtgac gtgttgatta tcggtagcgg cgcagccgga ctttcactgg
    10501 cgctacgcct ggctgaccag catcaggtca tcgttctaag taaaggcccg gtaacggaag
    10561 gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt tgatgaaact gacagcattg
    10621 actcgcatgt ggaagacaca ttgattgccg gggctggtat ttgcgatcgc catgcagttg
    10681 aatttgtcgc cagcaatgca cgatcctgtg tgcaatggtt aattgaccaa ggggttttgt
    10741 ttgataccca cgttcaaccg aatggcgaag aaagctacca tctgacccgt gaaggtggac
    10801 atagtcaccg tcgtattctt catgccgccg acgccaccgg tagagaagta caatccacgc
    10861 tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct ggagcgcagc aacgcggttg
    10921 atctgattgt ctctgacaaa attggcctgc cgggcacgcg acgggttgta ggcgcgtggg
    10981 tatggaaccg aaataaagaa acggtggaaa cctgccacgc aaaagcagtg gtactggcaa
    11041 ccggcggtgc gtcgaaggtg tatcagtaca ccaccaatcc ggatatttct tctggcgatg
    11101 gcattgctat ggcgtggcgc gcgggctgtc gggttgccaa tctcgaattt aatcagttcc
    11161 accctacagc actgtatcac ccacaggcac gcaatttcct gttaacggaa gcactgcgcg
    11221 gcgaaggcgc ttatctcaag cgcccggatg gcacgcgttt tatgcccgat tttgatgagc
    11281 gcggcgaact ggccccgcgc gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc
    11341 tcggcgcaga ttgtatgttc ctcgacatca gccataagcc cgccgatttt attcgccagc
    11401 atttcccgat gatttatgaa aaattgctcg ggctggggat tgatctcaca caagaaccga
    11461 taccgattgt gcctgctgca cactatacct gcggtggtgt aatggttgat gatcatgggc
    11521 gtacggacgt cgagggcttg tatgccattg gcgaggtgag ttataccggc ttacacggcg
    11581 ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt ctatggctgg tcggcggcag
    11641 aagatatcac cagacgtatg ccttatgccc acggcgtcag tacgttaccg ccgtgggatg
    11701 aaagccgcgt tgagaaccct gacgaacggg tagtaattca gcataactgg cacgagctac
    11761 ggctgtttat gtgggattac gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc
    11821 tgcggcggat aaccatgctc caacaagaaa tagacgaata ttacgcccat ttccgcgtct
    11881 caaataattt gctggagctg cgtaatctgg tgcaggttgc ggaattgatt gttcgctgtg
    11941 cgatgatgcg caaggagagt cgtggcctgc atttcacgct ggattatccg gaactgctca
    12001 cccattccgg tccgtcgatc ctttcccccg gcaatcatta cataaacaga taaaaagcct
    12061 gggtcagcgc cgtatacgct tcggaatagt tctggtctgg cccacgaatg actaagcgat
    12121 cgctaaagca ttctcccgcc tgcggggaga atgccagcag cacccgatgc ggcagtcgcg
    12181 cttcgttttc cgccacatcc gtccgcaaac gtaaatgcca gcccatgctt aatgccagct
    12241 ccgtaaaacc attaccaatc tgctctggca gcactacgca gaaaaatccc tcttcggtaa
    12301 tgcactccgc cgcacaggtc agcagcgatg ggtgatcaag cgtagtggta tagcgagcct
    12361 gttcccgttg aggggtcgcg cactctactc cctgctgata gtaaggtggg ttactgatga
    12421 ttaaatcgaa gcgtactgtc tgctgtgtga tccactgctg aatatccgcc gtatggacgt
    12481 taatccgctc tgcccacggg gactggttga tattttcctg cgcctgcgcg gcagcttcac
    12541 tttccagctc aacggcatca attatcacgc tgtcatcggt tcgctgcgcc agcattaatg
    12601 ccagcaaccc gctacccgcg ccgatatcaa ggcaacgttt caccccagcc accggtgccc
    12661 atgcgcccag caaaatgcca tccgttccca ctttcatcgc acagcgatcg tgagcaacaa
    12721 aaaactgttt aaaagtaaat ccattacgac gaagcacgga tgtagactgt gacatgaaaa
    12781 taaaaccttg caggaaaaac ggcgatagca ccgggtgaga acaatacccg agaagcgata
    12841 tccatacaaa cagatgaaga ttgcagccgt aacgtctata atcagcgccc cacacagagg
    12901 tagaacatga ctgtaacgac tttttccgaa cttgaactcg acgaaagcct gctggaagcc
    12961 ctccaggata aaggtttcac tcgcccgacc gccattcagg ctgccgccat tccgcctgcg
    13021 ctcgatggcc gtgatgtact cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat
    13081 ctgctgccag cgttgcagca cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt
    13141 attttgatcc tcaccccaac tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa
    13201 ctggcgaaac atacgcatct ggatatcgcc accatcaccg gcggcgtagc ctatatgaac
    13261 cacgcggaag tgttcagcga aaatcaggac atcgtggtcg ccacgaccgg acgtctactg
    13321 caatacataa aagaagagaa cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa
    13381 gcagaccgta tgctggatat gggctttgct caggatatcg aacatattgc tggcgaaacg
    13441 cgctggcgta aacagaccct gctcttttcg gcaacgctgg aaggcgatgc gattcaggac
    13501 tttgccgagc gtctgctgga agatccggtg gaggtttctg ccaatccgtc cacccgtgag
    13561 cgcaaaaaaa ttcatcagtg gtattaccgc gccgatgacc tggaacataa aaccgcattg
    13621 ctggtgcatc tgttaaaaca gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt
    13681 gaacgtgtgc atgagctggc aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc
    13741 gaaggtgaga tggtacaagg caagcgtaac gaagcgatca agcgtttgac cgaaggtcgc
    13801 gtaaacgtac tggttgcaac cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc
    13861 cacgtcttta acttcgatat gccgcgcagt ggcgatactt atttgcaccg tatcggacgt
    13921 accgcgcgcg ccggtcgtaa aggcaccgca atttcgctgg tggaagccca tgaccatttg
    13981 ctgctgggca aggttggccg ttacattgaa gagccgatta aagctcgcgt tattgatgag
    14041 ttacgcccga aaacgcgtgc gccaagcgag aagcagaccg gcaagccatc gaagaaagta
    14101 ctggcaaaac gcgccgagaa gaaaaaagct aaagagaaag agaaaccgcg tgtgaaaaaa
    14161 cgccatcgcg acaccaaaaa tattggtaag cgccgtaaac caagcggaac gggcgtgcca
    14221 ccgcaaacga cagaagagta atctcaatgc cgggtttaag cctggtatta aaagtgctgg
    14281 caaacgcaaa ac
//
LOCUS       DAMZII010000122        14192 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZII010000122 DAMZII010000000
VERSION     DAMZII010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.122"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            178..1749
                     /gene="garD"
                     /locus_tag="POA77_003681"
     CDS             178..1749
                     /gene="garD"
                     /locus_tag="POA77_003681"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDI6367048.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1898..2233
                     /gene="prlF"
                     /locus_tag="POA77_003682"
     CDS             1898..2233
                     /gene="prlF"
                     /locus_tag="POA77_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDI6367049.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2233..2697
                     /gene="yhaV"
                     /locus_tag="POA77_003683"
     CDS             2233..2697
                     /gene="yhaV"
                     /locus_tag="POA77_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDI6367050.1"
                     /translation="MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH"
     gene            complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="POA77_003684"
     CDS             complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="POA77_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDI6367051.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3810..5090
                     /gene="kbaZ"
                     /locus_tag="POA77_003685"
     CDS             3810..5090
                     /gene="kbaZ"
                     /locus_tag="POA77_003685"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDI6367052.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5113..5586
                     /gene="agaV"
                     /locus_tag="POA77_003686"
     CDS             5113..5586
                     /gene="agaV"
                     /locus_tag="POA77_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI6367053.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5597..6376
                     /locus_tag="POA77_003687"
     CDS             5597..6376
                     /locus_tag="POA77_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDI6367054.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6366..7244
                     /locus_tag="POA77_003688"
     CDS             6366..7244
                     /locus_tag="POA77_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDI6367055.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7262..7696
                     /locus_tag="POA77_003689"
     CDS             7262..7696
                     /locus_tag="POA77_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6367056.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7693..8826
                     /gene="nagA"
                     /locus_tag="POA77_003690"
     CDS             7693..8826
                     /gene="nagA"
                     /locus_tag="POA77_003690"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6367057.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9177..10331
                     /locus_tag="POA77_003691"
     CDS             9177..10331
                     /locus_tag="POA77_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDI6367058.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10344..11204
                     /gene="kbaY"
                     /locus_tag="POA77_003692"
     CDS             10344..11204
                     /gene="kbaY"
                     /locus_tag="POA77_003692"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDI6367059.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11371..11847
                     /gene="agaB"
                     /locus_tag="POA77_003693"
     CDS             11371..11847
                     /gene="agaB"
                     /locus_tag="POA77_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI6367060.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11886..12689
                     /gene="agaC"
                     /locus_tag="POA77_003694"
     CDS             11886..12689
                     /gene="agaC"
                     /locus_tag="POA77_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDI6367061.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12679..13470
                     /gene="agaD"
                     /locus_tag="POA77_003695"
     CDS             12679..13470
                     /gene="agaD"
                     /locus_tag="POA77_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDI6367062.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13471..>14192
                     /locus_tag="POA77_003696"
     CDS             13471..>14192
                     /locus_tag="POA77_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDI6367063.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWL"
BASE COUNT         3422 a         3598 c         3714 g         3458 t
ORIGIN      
        1 aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctgaa atgattgaca
       61 ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg aagtcattta
      121 tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata tagtacaatg
      181 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
      241 gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat
      301 ggactggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg
      361 gctaatggtg aaattattcg ttatggtgaa gtgattggtt acgccgtgcg tgcaatccca
      421 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
      481 ctgccactgg caaccaaagt cccggccccc ttaccgccgc tggaaggata cacctttgag
      541 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
      601 gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg cgatctgcta
      661 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ctgtggcgtg
      721 gcgattaacg caccggcggc agttgtgcct attcgtacca tccacaatat ttcgctgaac
      781 cctaactttg gcggcgaagt aatggtgatt ggtctgggtt gtgaaaagtt gcagcctgag
      841 cgcctgctgg tcggcacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc
      901 agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc
      961 gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa
     1021 ctggttgtcg gcatgcagtg cggtggcagc gatgcctttt ctggcgtaac ggcaaacccg
     1081 gcggttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat gttctcagaa
     1141 gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     1201 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc
     1261 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa cgtggtagag
     1321 aaggcactcg gctccattgc taaatcgggt aaaagcgcaa ttgttgaagt gctgtcgccc
     1381 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     1441 tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt
     1501 acgccgtacg gcctgatggc ggtacccgtc attaaaatgg cgacccgcac cgagctggcg
     1561 aaccgctggt ttgatttaat ggatattaac gcgggcacta tcgccaccgg cgaagaaacc
     1621 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     1681 aaaaccttct cggatcaatg ggggctgcat aaccagctgg cagtgtttaa cccggcaccg
     1741 gtgacctgat ttcttttgga acgtccctcg caaaacatgg ccttagtgcc atgtttttat
     1801 tgtttaaagc ccccacgtcc attaataatg catttgcatt accttagttc aagcttataa
     1861 tttgagcaga aaacaggctg taaaaggaca gtgaatcatg cccgctaatg ctcgctctca
     1921 cgctgtactg accactgaat caaaggtcac gatacgcgga caaacaacta tccccgcgcc
     1981 agtgcgtgag gccttaaaac tgaagccagg ccaggacagc attcattacg aaattctgcc
     2041 tggtgggcaa gtctttatgt gccgactggg agatgaacag gaggatcata ctatgaatgc
     2101 atttttgcgt tttctggatg cagatatcca gaacaacccg caaaaaactc gtccattcaa
     2161 cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg
     2221 cgacgacgaa taatggattt tccacaaagg gttaatggtt gggtgctata tgctcatccc
     2281 tgttttcagg aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat
     2341 cctgaaaatt atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt
     2401 gaggagcata tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc
     2461 tctgggaaaa ataaagactg gtcacgggta aaatttggtg ctggtcgtta tcgtctcttc
     2521 tttcgttata gtgaaaaaga gaaagtcatc attctgggat ggatgaacga tgaaaacact
     2581 ctgcgcacct acggtaaaaa aacagatgcc tataccgtat tcagcaaaat gttaaaaaga
     2641 ggacatcctc ctgccgactg ggaaagcttc acccaagaga cagaagaaaa ccattgatgg
     2701 tgttcacaat gcccgccgaa cgtattcgtg ttgaccagcg ggcacccttt ctcactcccc
     2761 gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg caggaatgcc
     2821 ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggaacg
     2881 attgaattta ctggaatcgg tgaccacgat gatccgttcc gcaacttcgc acatccgacg
     2941 gtttaaacgg gcttcatctt cattatgtgt gctgacgccg cgctccagat cgatcgcatc
     3001 tacaccaaga aacagcatat cgaagtggta attttgcagc gattgctcag cctgatcgcc
     3061 gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc
     3121 cgcttccagc aacgcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgtg
     3181 cttacgcatc agacgagcaa tctcaaaagt ggtagtcccg gaatcgagga tcacccgatg
     3241 accaggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg cggtgttcag
     3301 cgcgctttta tcttccactg atggctcgac tgacggcgtc gtgctatcgc agatcaacgc
     3361 gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat cattgcggat
     3421 cgtcacagta gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgctg
     3481 tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg ccagtcactc gcttctcacc
     3541 tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt
     3601 gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg acgcaagcta
     3661 ctctcgtttc agtgactttc attatgtttc ttttgtgaat cagatcagaa aactattatc
     3721 tttcgtttta tttttatctc accatgacgc agtatcaact gaaacaaaac gaaagattaa
     3781 tatcgcagta atctgaactg gagaggaaag tgaaacatct gacagaaatg gtgagacagc
     3841 acaaagcggg caaaacaaat ggaatttatg ccgtttgttc cgcacatccg ctggtgctgg
     3901 aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa gcaacctcca
     3961 atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt cgcggctttg
     4021 tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gttgattctg ggtggtgacc
     4081 atctggggcc aaaccgctgg caaaacctgc cggccgctca ggcaatggcc aatgccgatg
     4141 atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt
     4201 cctgtcagga cgatccgatt cccttaactg atgacatcgt ggctgaacgc gccgcccgtc
     4261 tggcgaaagt ggcggaagaa acctgtcttg aacactttgg cgaagccgat ctggagtatg
     4321 tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc gagctggcgg
     4381 tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc
     4441 aaggtttgaa tgccatctgg ccacgcatca ttgccctggt ggttcaaccc ggcgtcgaat
     4501 tcgatcacac caacgttatt gattatcagc ccgccaaagc gagcgcctta agccagatgg
     4561 tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa acgccgcaat
     4621 cgctgcgcca gctggtgatt gaccactttg ccattctgaa agttggccca gcgctgacct
     4681 tcgccctgcg tgaagctctg ttctctctgg cggcgattga agaagaactg gtgccagcga
     4741 aagcctgttc tggtctgcgt caggtgctgg aagacgtgat gctcgaccgc ccggaatact
     4801 ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt tatagctact
     4861 cggatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc gctcatctgg
     4921 ttcgtaatct ggcggattca ccaattccgc taccgctcat cagccagtat ctgccgctgc
     4981 aatacgtgaa agttcgctcc ggcgagctgc aaccgacgcc acgggaactc attatcaacc
     5041 atattcagga catcctggcg cagtactaca cagcctgtga aggccaataa gcaaaacaaa
     5101 gaggaacacg ctatgccaaa tattgtttta agccggattg atgaacgctt gattcacggt
     5161 caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc tggtgctggt agccaacgat
     5221 gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc agaagggatc
     5281 gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga
     5341 cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt gaaaggtggc
     5401 gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa acaacaaatc
     5461 gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct gaaagccgct
     5521 ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct ctttaaatta
     5581 ctttgaggga ttcatcatgg aaatcagcct gttgcaggca tttgcgttgg gcattatcgc
     5641 ctttatcgct ggcctggata tgtttaacgg cttaacccat atgcaccgcc cggtggtcct
     5701 cggcccgttg gtcgggctgg tacttggcga tctgcatacc ggaattttaa ccggcggtac
     5761 gctggaactg gtgtggatgg ggctggcccc gctagcgggc gcacagccgc ctaacgtgat
     5821 tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc
     5881 ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct tcctgttctc
     5941 ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg atactcgcgg
     6001 cattgaacgc gtgaactatc tggcactgct ggcactcggc actttctatt ttctctgcgc
     6061 tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg tcctgccgca
     6121 acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt
     6181 gctgctgaaa atcatgatga aaaacgtcta catcccctac ttcatcctgg gctttgttgc
     6241 cgcagcctgg ctcaagttac cggtgctggc tattgctgcc gctgcgctgg cgatggcgct
     6301 gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga aagaggaatt
     6361 tgaagatggc atctaatcaa accaccctgc cgaacgtcag tgaaaacgaa gaaacattgc
     6421 tgactggcgt caatgaaaac gtgtatgaag atcagagcat tggcgcggag ctgacgaaaa
     6481 aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca agcgtctttt aactacgaac
     6541 gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc tctgaaaaag atccacacta
     6601 ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat acccatccgt
     6661 ttctggtgac atttgttatc ggcattatcc ttgcgatgga gcgttctaag caggacgtta
     6721 acagtattca gagcaccaaa attgccgtcg gtgcgccgct cggcgggatt ggtgatgcga
     6781 tgttctggct gacgctactg ccgatttgtg gcgggatagg cgcaagcctt gcgctacaag
     6841 gctctattct tggcgcagtc gtatttattg tgctgttcaa cgtggtacac ctggggctgc
     6901 gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattcca ctcattaaag
     6961 cgaataccaa aaaagtcggc catgctgcgt ctatcgttgg gatgacggta atcggcgcgc
     7021 tggtggcaac ctatgttcgt ttaagcacca cgctggaaat cactgcgggc gacgcagtgg
     7081 ttaagttaca gactgatgtt attgacaaac tgatgcctgc cttcttaccg ctggtctaca
     7141 ccttgaccat gttctggctg gtacgccgcg gctggagtcc gctgcgtctg attgcagtta
     7201 ccgtggttct cggcatcgtc ggtaaattct gccatttcct ttaaatacaa tgaggtatta
     7261 gatgttaagt attattttga cagggcatgg cgggtttgcc agcggcatgg aaaaagcaat
     7321 gaagcaaatt ctcggtgagc aatcgcagtt tatcgccatc gattttccgg aaacctcaag
     7381 caccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt gtgaagacgg
     7441 cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt caacgctggc
     7501 gatgcaaaaa ccgggctgtg aagtgatcac cggcaccaat ttgcaactgc tactggagat
     7561 ggtgctggag cgcgaagggt taagcggtga agaattccgt gtacaggcgc tggagtgtgg
     7621 acaccgaggg ctgaccagtc tggtggacga gttaggccgc tgccatgaag aatgtccggt
     7681 cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga agagggatgg
     7741 ctcgatgacc atcagttgcg tattgctgac ggtgtcatcg cagcaatcga accgattcca
     7801 gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg cttacatcga cacccatgta
     7861 cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga caagctggca
     7921 atgcacaagg cacgcgaagg tgtcggcagt tggttgccga ctaccgtaac cgcgccgctt
     7981 aacagcattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt
     8041 gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg
     8101 catccgccgg aattgtttcg cgagcttgaa attgccgagc tggatcaatt gattgccgtt
     8161 tctcagcaca ccttacgcgt ggtagcgctg gcaccggaaa aagagggggc attgcaggcc
     8221 atccgccatc ttaaacagca aaatgtacga gtgatgctgg ggcatagcgc ggcgacctgg
     8281 caacaaactc gcgccgcgtt tgatgctggt gccgacggcc tggtgcattg ctataacggg
     8341 atgacaggtt tacatcaccg cgaaccggga atggttggcg cgggattaac ggacaagcgc
     8401 gcctggctgg aactgatagc cgatggtcat catgtgcatc cggcggcgat gtcgctgtgt
     8461 tgttgctgtg caaaagagag aatcgtgctg atcaccgacg cgatgcaggc agccgggatg
     8521 ccggatggtc gctatacgtt atgtggcgaa gaagtgcaga tgcacggtgg cgttgtccgt
     8581 accgcgtccg gtgggctggc gggcagtacg ctgtctgttg atgcggcagt gcgcaacatg
     8641 gtcgagttga cgggcgtaac gcctgcggaa gccattcata tggcatcgct gcatccggcg
     8701 cgaatgctgg gtgttgatgg tgttctggga tcgcttaaac cgggcaaacg cgccagcatc
     8761 gttgcgctgg atagcgggct gcatgtgcaa caaatctgga ttcagagtca attagcttcg
     8821 ttttgatatt ttgctccttt attgggcctt cacttccccc gtaaggcctt tctttttctt
     8881 tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaac gcgtcttatg cgacctacgg
     8941 tacgccgcat ccagcaatcg gtgaacatag cgacgtcatt acgcaattct ttaatcttcc
     9001 cttgtctttt ctttcaatga tcacagattt cgttttattt cctttttctc cattgaactt
     9061 tcggtttctt ttctatagat tttaatcaac gaaagacatc accaagtgaa atgaaacgaa
     9121 aggcaagtga aagcgacaac gcccgacgtc aagttcatca gactaaggat tgagttatgc
     9181 cagaaaatta cacccctgct gccgccgcaa ccggtacatg gactgaagaa gagatccgcc
     9241 atcagcctcg cgcatggatc cgttcactca ccaacatcga cgcgctacgt tctgcgctca
     9301 ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg accggagccg
     9361 gaacctcggc atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa
     9421 acttcagcgc cgtaccgacc accgatctgg tcactaatcc gatggactac ctgaacccag
     9481 cccatccgct gctgttgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgctg
     9541 ccgtggaact ggcaaatcaa tttgtaccag aatgctatca cctgccgatc acctgcaacg
     9601 aagcgggcgc tctttaccaa aacgcgatca acagcgataa cgcgtttgcc ctgctgatgc
     9661 ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag cattaccacc atgatggcca
     9721 gctgcctcgc ggttttcgca cctgagacga tcaacagcca aaccttccgc gacgtggcgg
     9781 atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg
     9841 caccgtggaa acggatcgtt tatctcggca gcggtggctt acagggcgca gcacgcgagt
     9901 cggcgctgaa agtgctggaa ctgactgcgg gtaaactggc ggccttctat gattccccga
     9961 ccggattccg tcatggcccg aaatcactgg tcgataacga aacgctggtg gtggtatttg
    10021 tctcaagcca cccttacacc cgtcagtatg atcttgatct gctggctgaa ctccgccgtg
    10081 acaaccaggc aatgcgcgta atcgccatcg ccgcggaaag caccgacatc gtcgctgccg
    10141 gtccacatat tatcctgccg ccgtcacgtc actttatcga cgttgagcag gcattttgct
    10201 tcctgatgta cgcccagacg tttgcactga tgcagtcgct gcacatgggc aatacgccgg
    10261 ataccccatc agccagcggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt
    10321 ggcaggcata agaggatcgc attatgagca ttatctccac taaatatctg ttacaggacg
    10381 cccaggccaa tggctacgcg gtgcctgctt ttaacattca taacgccgag acgatccagg
    10441 cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga acgccgggga
    10501 cctttaaaca tatcgcgctg gaagagatct acgccctgtg cagcgcctat tccacaacct
    10561 acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat attcgccgta
    10621 aagtccacgc aggtgtgcgc agtgcaatga tcgacggcag ccacttcccg tttgccgaga
    10681 acgtgaagct ggtgaaatcg gttgttgact tctgccactc acaggattgc agcgtggaag
    10741 cagaactggg ccgcctgggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat
    10801 tcctgaccga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc gacagcctgg
    10861 cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc
    10921 ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctacatggt gccagcgatg
    10981 ttccggatga atttgtccgt cgcactattg aacttggcgt cacaaaagtg aacgttgcca
    11041 cagaattaaa aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg
    11101 gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa
    11161 ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg
    11221 atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggaa aaggagatga
    11281 ctaaacctta ataaatacat cagtacaata tcgcaacaat aatatattaa aaaaaattat
    11341 attattcaac tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt
    11401 attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgcg
    11461 aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg
    11521 ggtattaccg cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt
    11581 aacgtcatcg gcaaagctgc accgcatcag aagatcttcc tgatttgccg tacgccacaa
    11641 acggtacgta aattggtaga aggtggcatt gacctgaaag atatcaacgt cggcaatatg
    11701 catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc
    11761 gcggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc
    11821 gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa
    11881 taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta gttttcgttc
    11941 tggggattga tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc
    12001 taactggcgc tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt
    12061 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg
    12121 gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc
    12181 ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct
    12241 cattatttat gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc
    12301 ggcttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct
    12361 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga
    12421 cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc
    12481 gcgtaatgtt caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct
    12541 acatccaggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg
    12601 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
    12661 aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc
    12721 tttcgttcgt cgctgctgca agcgagcttt aactatgaaa ggatgcaggc gggcggtttt
    12781 acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc
    12841 gcggcgatga aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg
    12901 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc
    12961 aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg
    13021 ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg
    13081 attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
    13141 ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct
    13201 cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat
    13261 attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat
    13321 ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac
    13381 ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct
    13441 attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc
    13501 tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg
    13561 ttaagtgaac gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg
    13621 atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc
    13681 caccagcagc aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat
    13741 ctgccattaa cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg
    13801 ctggggctgc gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag
    13861 tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctagga
    13921 ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc
    13981 catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc
    14041 cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg
    14101 ttattggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc
    14161 tccaccgcta tcccggcttc gtttttatgg ct
//
LOCUS       DAMZII010000123        14180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZII010000123 DAMZII010000000
VERSION     DAMZII010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.123"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            167..1225
                     /gene="citC"
                     /locus_tag="POA77_003697"
     CDS             167..1225
                     /gene="citC"
                     /locus_tag="POA77_003697"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDI6367064.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCNWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1240..1536
                     /gene="citD"
                     /locus_tag="POA77_003698"
     CDS             1240..1536
                     /gene="citD"
                     /locus_tag="POA77_003698"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDI6367065.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1533..2441
                     /gene="citE"
                     /locus_tag="POA77_003699"
     CDS             1533..2441
                     /gene="citE"
                     /locus_tag="POA77_003699"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDI6367066.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2452..3984
                     /gene="citF"
                     /locus_tag="POA77_003700"
     CDS             2452..3984
                     /gene="citF"
                     /locus_tag="POA77_003700"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDI6367067.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3988..4539
                     /gene="citX"
                     /locus_tag="POA77_003701"
     CDS             3988..4539
                     /gene="citX"
                     /locus_tag="POA77_003701"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDI6367068.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4514..5392
                     /gene="citG"
                     /locus_tag="POA77_003702"
     CDS             4514..5392
                     /gene="citG"
                     /locus_tag="POA77_003702"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDI6367069.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5443..6906
                     /gene="citT"
                     /locus_tag="POA77_003703"
     CDS             5443..6906
                     /gene="citT"
                     /locus_tag="POA77_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDI6367070.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7020..7826
                     /gene="rna"
                     /locus_tag="POA77_003704"
     CDS             7020..7826
                     /gene="rna"
                     /locus_tag="POA77_003704"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDI6367071.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8056..8466
                     /gene="rnk"
                     /locus_tag="POA77_003705"
     CDS             8056..8466
                     /gene="rnk"
                     /locus_tag="POA77_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDI6367072.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8697..9935)
                     /locus_tag="POA77_003706"
     CDS             complement(8697..9935)
                     /locus_tag="POA77_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDI6367073.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10156..10584
                     /gene="uspG"
                     /locus_tag="POA77_003707"
     CDS             10156..10584
                     /gene="uspG"
                     /locus_tag="POA77_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDI6367074.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10705..12270)
                     /gene="ahpF"
                     /locus_tag="POA77_003708"
     CDS             complement(10705..12270)
                     /gene="ahpF"
                     /locus_tag="POA77_003708"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI6367075.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12399..12962)
                     /gene="ahpC"
                     /locus_tag="POA77_003709"
     CDS             complement(12399..12962)
                     /gene="ahpC"
                     /locus_tag="POA77_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDI6367076.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13334..14080
                     /gene="dsbG"
                     /locus_tag="POA77_003710"
     CDS             13334..14080
                     /gene="dsbG"
                     /locus_tag="POA77_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDI6367077.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3361 a         3753 c         3572 g         3494 t
ORIGIN      
        1 aatttcacgc cgaaatactc cttaggatgt atagcgaaag gagaaaaaga tatacctcga
       61 tcacccccct ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat
      121 tcgttgcaaa tcgataacaa catgcacctt caggatacta tttattatgt tcggcaatga
      181 tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct
      241 gcatgaaaat gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga
      301 tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag
      361 tgaatccgtt cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc
      421 ctatgagcgg cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt
      481 ccgccagtgc ggtttttcca cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa
      541 cagcgccacg cggctgaaac gctatgccga atcgctgaaa aaatttcgtc atccagggaa
      601 caagattggc tgcattgtga tgaacgccaa tcccttcacg aatggtcacc gttatctgat
      661 tcaacaagct gcggcacagt gcaactggtt gcatctgttt ttagtcaaag aagattcttc
      721 acgcttcccc tatgaagacc ggttggatct ggtgttaaaa ggcaccgccg atattccacg
      781 cctgactgtg catcgcggct ccgaatacat catctcccgc gctacgttcc cttgctactt
      841 cattaaagaa cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg
      901 tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt gtcggtactg aacccttttg
      961 tcgcgttacc gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc
     1021 cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc
     1081 cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt
     1141 ccctgcagtc acgctgcatt atttgcagaa cctgcttgag cactcccgcc aggacgcggc
     1201 agctcgtcaa aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc
     1261 gttgcaggca cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag
     1321 gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc
     1381 accattctgg acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac
     1441 aaaggcgcac tggactgcat tttacgtgca cgactggaag ccctgctggc acgcgccagc
     1501 ggtatcccgg ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta
     1561 aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca
     1621 actccttcat ctacccggct gatgccctga tgtttgacct cgaagactcc gtagcattgc
     1681 gtgaaaaaga caccgcccgc cgcatggttt accacgcgct gcaacatccg ctgtatcgcg
     1741 atattgaaac cattgtgcgt gtcaacgcgc tggattccga atggggtgtc aacgacctgg
     1801 aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg
     1861 atgttctgga tatcgaaaaa gagatcctgc gtatcgaaaa agcctgtggt cgtgaacccg
     1921 gcagcaccgg cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa
     1981 tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca
     2041 acctgcgtac agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt
     2101 tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca
     2161 acgaagccgg attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat
     2221 cgctgatcaa cccgcgtcag attgatctgt tgcacaacct ctacgcacca acccagaaag
     2281 aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg
     2341 gcgtggtttc cctgaacggc aagatggtgg acggtccggt tatcgatcgc gcccgtctgg
     2401 tgctctctcg tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag
     2461 aaaattgaac aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc
     2521 gatcttgccg ctttccagaa ctcgccaaag caaacctacc aggctgaaaa agcgcgcgat
     2581 cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct ctggtttaca ggacggcatg
     2641 acggtttcct tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac
     2701 gtcatcgcga agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc
     2761 catgcgccgt tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctccggc
     2821 ctgcgcggtc cactggcgga agagatctcc cgtggtctgc tggcagaacc ggtgcaaatc
     2881 cactctcacg gcggtcgcgt gcatctggta cagagcggcg aactgaatat cgacgtggct
     2941 ttcctcggcg tcccgtcctg cgatgaattc ggtaatgcca acggctacac cggtaaagcc
     3001 tgctgcggct ccctcggcta tgcaatggtt gatgccgaca acgcaaagca ggtcgtgatg
     3061 cttaccgaag aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt
     3121 gatttgatcg tcaaagttga ccgcgttggc gatgctgcaa aaatcggcgc tggcgcgacc
     3181 cgtatgacca ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc
     3241 aactcaggct acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg
     3301 gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc
     3361 cttggcggta ttaccgcgac gatggttgac ctgcacgaaa aaggtctgat ccgcaaactg
     3421 ctggatgtgc agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac
     3481 atcgaaatca gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt
     3541 ctcgacgtgg tggtactgag cgcgctggaa attgacaccc agttcaacgt taacgtgctg
     3601 accggctctg acggcgtact gcgtggtgct tccggtggtc actgcgatac cgcgattgcc
     3661 tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc gtattccgac tctggtggat
     3721 aacgtgctga cctgcatcac cccgggctcc agtgtcgata ttctggtcac agaccacggt
     3781 atcgcagtta acccggcacg tccggaactg gcagaacgtc tgcaggaagc gggcattaaa
     3841 gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg
     3901 attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat
     3961 gttgtgcatc aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat
     4021 gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc
     4081 tggctcaagc gccatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt
     4141 aaagacagcg aggtcacacg ccgaattttt aatcatggcg tgacagcctt gcgtgcctta
     4201 gccgcaaaac agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg
     4261 gagggcatgt tgagcattgc cgccccggct cgcgacctca agctcgccac cattgagctt
     4321 gaacatagtc atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa
     4381 attctctccc gccgcgacta ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc
     4441 gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg atttactcaa ccgcatggag
     4501 gcactgctga acgatgtcga tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc
     4561 attaatcgat gagtacgccc tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc
     4621 accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg cacaaagata tggcgctgga
     4681 agatttccac cgcagcgcgc tggcgattca gggctggtta ccccgtttca ttgaatttgg
     4741 tgcctgtagt gcagaaatgg caccggaagc ggtactccac ggattacgcc caattggtat
     4801 ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta aacacgcata aaggcagcat
     4861 tttttcttta gggctgctat gtgcggcaat tggccgtttg cttcagctca accagccggt
     4921 aacgccaaca accgtttgtt ctacggcggc aagtttctgc cgtggtctga ccgatcgcga
     4981 actgcgtacc aataattcac aactgacggc aggtcaacgg ttgtaccagc agcttggcct
     5041 taccggcgca cgcggtgaag ccgaagcggg ttatccactg gtgatcaacc acgccttgcc
     5101 gcattacctc actctgctgg atcaggggtt agatcctgaa ctggcattgc tcgatacctt
     5161 gctcctactg atggcgatca acggcgatac caacgttgct tcgcgcggtg gcgagggggg
     5221 cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa aaagggggca ttcgaacccc
     5281 cgccgatctc gattatctcc ggcagttcga cagggagtgt atcgaacgaa atctcagtcc
     5341 aggcggcagt gccgacctgc tgatccttac ctggttttta gcacagattt aattatttaa
     5401 gcacttgata aatttgaaaa tattaatttt cggagaaccc gtatgtcttt agcaaaagat
     5461 aatatatgga aactattggc cccactggtg gtgatgggtg tcatgtttct tatccctgtc
     5521 cccgacggta tgccaccaca ggcatggcat tacttcgctg tgtttgtggc aatgattgtc
     5581 ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt ttattgcggt tactatttgc
     5641 gttattggca gtaattacct gctctttgat gccaaagaat tagctgaccc agcgtttaat
     5701 gcgcaaaaac aggcgctgaa atggggtctg gctggttttt ccagcaccac ggtatggctg
     5761 gtatttggcg catttatttt tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt
     5821 gcccttttcc tggtgaaatt catgggcaaa cgcacgctga cgttgggtta tgcgattgtc
     5881 attatcgaca ttctgctggc accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg
     5941 gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat cattccccaa cgatccgtcc
     6001 gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca ttagtaccag tctgagttcg
     6061 tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt
     6121 gccggtatcc agattagctg gttgcagtgg ttcctctgct tcctgccggt tggggttatc
     6181 ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca aaccggaaat cacacacagt
     6241 gaagaagtgg caacctgggc gggtgatgaa ctgaaaacca tgggtgcgct gacacgcaga
     6301 gagtggacac tgattggcct tgtattgctc agcttaggtt tgtgggtttt tggcagtgaa
     6361 gtcattaatg ctactgcagt tggtctgctg gcagtttcgc taatgctggc cctgcacgtt
     6421 gtgccttgga aagatattac ccgctataac agcgcatgga acacgctggt caacctggca
     6481 actctggttg tgatggctaa cggcctgact cgttctggct ttattgactg gttcgccggt
     6541 accatgagta cgcacctgga aggattctca ccaaacgcaa cggtgattgt actggttctg
     6601 gtgttctact ttgcacacta cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg
     6661 ccggttattc tggccgtcgg taaaggtatt ccgggcgtac caatggaaca actgtgtatc
     6721 ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg
     6781 gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc
     6841 ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct cgccatgtgg
     6901 aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtta gcccacctgg
     6961 cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc acacccatta
     7021 tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg
     7081 ccaacgccgt agcgttgcag gcaaaacagt atggcgattt tgatcgctat gtcctggccc
     7141 tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac gaacgagatg
     7201 aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta catggtctgt
     7261 ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc tggatgcgct
     7321 tcggttgcgc tactcgccca atcccgaatc taccagaagc acgcgccagc cgaatgtgtt
     7381 catcgccgga aaccgggtta tcactggaaa cggccgctaa actaagtgaa gtcatgccag
     7441 gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt gcctgctttg
     7501 gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa attaaagaga
     7561 gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc cgtgactttg
     7621 acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc
     7681 agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac gccatcaacg
     7741 ctccgctttc tgcaaactca ttcttgccac aaccacaccc aggcaactgt ggcaaaacct
     7801 ttgtgattga taaagtgggt tattaatcat ctgatttaca aaacacgcta attcgtcttc
     7861 cataaaccgt gacgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg
     7921 tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc
     7981 gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga
     8041 cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc
     8101 atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac
     8161 gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca
     8221 atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg
     8281 gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt
     8341 gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt
     8401 gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactacctg
     8461 ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac gcgcatcctc
     8521 tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt
     8581 ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata ggatttctga
     8641 ttatggcggc gattatcgtc atggctgtcc tttacctcga aaaccactcc tgacgatcat
     8701 attgttcccc ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg
     8761 ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc
     8821 tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc
     8881 agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt
     8941 cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat
     9001 ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactgg
     9061 cgcaacgctt taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg
     9121 ctgcctttcg cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc
     9181 gtttgttcaa taattgactg tgccggatcg ctatcttcat cgaaattaat cgggatcgcg
     9241 ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat gatcaacaac aaaaatctgt
     9301 tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc ccactggacc ggcaccatag
     9361 actgcaacgc ttgaaccttg ttggatctgc gcattttttg ctgcctgcca tgccgttggc
     9421 agaatatcag aaaggaaaag ggctttatca tctgaaagca aaggcggtac tttaaacggc
     9481 cccacattcc ctttagggac gcggacatat tccgcctgcc caccaggaac gccgccatac
     9541 aggtgactat aaccaaacaa tgccgcggga gctggtatct gttttttatt gagcgcagcg
     9601 cctttacccg cattggtatt ttcgcaggcg gcatattgct gtaatcgaca gaaaaaacag
     9661 tcgccacaag caatgacaaa cggaattacc acccggtcgc ctttttgcaa attttttacg
     9721 tcctttccgg tttcaacgac ttcccccata aattcatgac caaaaatatc gccatgttta
     9781 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttgccgta
     9841 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
     9901 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
     9961 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    10021 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    10081 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    10141 cagggagaaa gggttatgta taagacaatc attatgccag ttgatgtatt tgaaatggaa
    10201 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    10261 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    10321 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    10381 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    10441 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttattgg ttctcgcaac
    10501 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccatgccaat
    10561 ctgccggtgc tggttgtgcg ttaattgcca gaataaatat cccgccctgc ccggagccat
    10621 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    10681 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    10741 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    10801 cgtacagtca cccgctgcga atacgccttt gacgttggtt tcgcatttcg catcaatgat
    10861 aatctcaccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag
    10921 accaatctgg acgaaaatac cggccagttc gatgttgtga atatcgccgc tgacacgatc
    10981 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    11041 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    11101 catttctggc gcaaattcga gcagagttac gtgctcaacg atacccgcca ggtcaattgc
    11161 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    11221 gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga tactgatctt cgccaggaac
    11281 gttcatgttg cgccattttg caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc
    11341 gccagaagct gtttcaatct gatgcagacc accttcaact gctgctggga tcagtttgct
    11401 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    11461 tgccagcttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    11521 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    11581 gtaaattgct gcagctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    11641 acgcttgttc agctcttctg ccgcacgttt ttccgcgccc gtatcaattt tggcaacgat
    11701 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    11761 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    11821 cttgatgcgc gggttcagta cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    11881 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    11941 ctccagtaga gactgcgctt ctttcgacgg atgaccaccg gtccatagca acgccagtac
    12001 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc
    12061 tgggttggtg atcaggaaag acggcttacg caccggcaag ctgttatctt ctttaaaggt
    12121 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    12181 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    12241 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    12301 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    12361 ggtgccgcag cacccgatta attacgcgat tgcggaactt agattttacc aaccaggtcc
    12421 agagacggag ccagagttgc ttcaccttct ttccatttag ccgggcaaac ttcacctggg
    12481 tgagaagcta cgtactgtgc tgctttgatt ttacgcagca ggtcagacgc gtcacggcca
    12541 atgccttcag cggtaacttc gattgcctgg atgataccct gcgggtcaac aacgaaggtc
    12601 gcacggtcag ccagaccttc atcttcacgc atgttgtcga agttacgggt cagggcgcca
    12661 gtcgggtcgc cgatcatcgc atatttgatt ttagcgatgg tttcagagct gctgtgccat
    12721 gctttgtggg tgaagtgagt atcggtagat actgcgtata cgtctacgcc cagtttctgc
    12781 agttcttcgt agtggtcagc aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca
    12841 gccgggtaga agaagaagac gctccagcgg ccttcggtat ctttttcggt gatttcgatg
    12901 aattcgccgt ttttgaatgc ctggttttta aaaggtttaa ttttggtgtt aatcaaggac
    12961 atctatactt cctccgtgtt ttcgatgaga tgtaaggtaa ccaatttttg ccgattcggc
    13021 taatgcgttt ccattatcaa atcgataagt tttaccttac aaccttcgta agacaacttt
    13081 gtgaactttg caccgccagc ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt
    13141 gtaatctgaa ctaccctttg gtatcttcag tgcctgcctg caggctgagg tgttgccctt
    13201 acaaatgcaa caacgacatg gattacaaca ccctcataaa caaagggcaa tcacctgatc
    13261 taagctctta cctatgacag tgataggtta tgccttttac tcgacttttg cactgactga
    13321 aaaggacaaa ttaatgttaa aaaagatact tttactggct ctgcttcctg caatcgcctt
    13381 cgcagaggaa cttcctgctc cagtaaaagc gattgaaaaa cagggcatta caatcatcaa
    13441 aacattcgat gcccccggag gaatgaaagg ttatctcgga aagtatcagg atatgggcgt
    13501 caccatctac ctgactccag atggtaagca cgctatctct ggttacatgt acaacgagaa
    13561 aggtgaaaac ctgagtaaca cacttatcga aaaagaaatt tacgcaccag ccggacgcga
    13621 aatgtggcaa cggatggaac aatcccactg gctcctcgac ggtaaaaaag atgcgccggt
    13681 cattgtctac gtcttcgccg atccgttctg cccatattgt aaacagttct ggcagcaggc
    13741 gcgcccgtgg gtagattctg gcaaagtgca attaagaaca ttgttggttg gggttatcaa
    13801 gccagaaagc ccggcgacag cagcggcaat tcttgcctcc aaagatcccg caaaaacctg
    13861 gcaacaatat gaagcctctg gtggcaagct taagctaaac gtgcctgcaa acgtaagtac
    13921 agagcaaatg aaagtgttaa gtgacaatga gaaactgatg gacgatctgg gggcaaatgt
    13981 cacgccggct atctattaca tgagtaagga aaatacgcta caacaggccg tggggttgcc
    14041 cgatcagaaa acgcttaata tcattatggg gaataaataa aagataattc atggatggtt
    14101 gaaatacatc tctgcttttc aaccatccta aaaaagcatg aattgctcgc tctgccctaa
    14161 gtggctatgc tattttagaa
//
LOCUS       DAMZII010000124        14004 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZII010000124 DAMZII010000000
VERSION     DAMZII010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.124"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(69..1115)
                     /gene="aroH"
                     /locus_tag="POA77_003711"
     CDS             complement(69..1115)
                     /gene="aroH"
                     /locus_tag="POA77_003711"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDI6367078.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1272..2105)
                     /gene="ppsR"
                     /locus_tag="POA77_003712"
     CDS             complement(1272..2105)
                     /gene="ppsR"
                     /locus_tag="POA77_003712"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDI6367079.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2438..4816
                     /gene="ppsA"
                     /locus_tag="POA77_003713"
     CDS             2438..4816
                     /gene="ppsA"
                     /locus_tag="POA77_003713"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDI6367080.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4873..6519)
                     /gene="fadK"
                     /locus_tag="POA77_003714"
     CDS             complement(4873..6519)
                     /gene="fadK"
                     /locus_tag="POA77_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDI6367081.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6576..6869)
                     /locus_tag="POA77_003715"
     CDS             complement(6576..6869)
                     /locus_tag="POA77_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI6367082.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6866..8155)
                     /locus_tag="POA77_003716"
     CDS             complement(6866..8155)
                     /locus_tag="POA77_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6367083.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8211..9149)
                     /locus_tag="POA77_003717"
     CDS             complement(8211..9149)
                     /locus_tag="POA77_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDI6367084.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9169..9933)
                     /locus_tag="POA77_003718"
     CDS             complement(9169..9933)
                     /locus_tag="POA77_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDI6367085.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDLSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            10249..11160
                     /locus_tag="POA77_003719"
     CDS             10249..11160
                     /locus_tag="POA77_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6367086.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(11203..12354)
                     /locus_tag="POA77_003720"
     CDS             complement(11203..12354)
                     /locus_tag="POA77_003720"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDI6367087.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12368..13963)
                     /locus_tag="POA77_003721"
     CDS             complement(12368..13963)
                     /locus_tag="POA77_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6367088.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQAQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         3411 a         3563 c         3517 g         3513 t
ORIGIN      
        1 agcagtaatg tgctgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
       61 gcacgcattc agaagcgggt atctaccgca gaggcaagtt tttcgaccag gcgttcggta
      121 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
      181 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatcgccgta
      241 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
      301 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
      361 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
      421 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
      481 tctggcgaga gaaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
      541 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgccga agccatttca
      601 cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat taaatcagca
      661 ataaactgac cggtcaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
      721 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
      781 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
      841 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
      901 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
      961 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
     1021 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
     1081 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
     1141 ctacggcagg actaccggga agtgatgcgc atcacgataa accaatcgca gctgatttca
     1201 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa ataagctaat
     1261 gcactagttc tctagtacat tcggcggcta agccccatga tatcgaggat cttggtggca
     1321 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaac
     1381 gcttctactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg gctgttctcc
     1441 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
     1501 tgctgaagcg gtttgagcga cgcgggtagc accagattgt ccatatcgtc ggcaataaag
     1561 gggtagtttg ccgcgcggat accaaactgc attgccagat acagactggt gggggtttta
     1621 ccacagcgag aaacaccgag caggatcacc tgagcctggt ccagattgcg caacgaaatg
     1681 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
     1741 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
     1801 tgttgtagcg gggcaaccag cgcctgaaca atatcctggc aaaagccttc actttgcaag
     1861 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacacctgtc
     1921 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
     1981 aacggcagcg taatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
     2041 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
     2101 tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat taaaaacgca
     2161 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcaatttta ttttttatga
     2221 aaacagcatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga aattgtcgaa
     2281 tagagcaggt ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata tatttgcttg
     2341 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
     2401 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
     2461 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
     2521 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
     2581 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
     2641 catttatgaa ctgctggata aaacggatat tgacgatgtt acccagcttg cgaaagcggg
     2701 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaatgccat
     2761 ccgcgaagct tatgcacagc tttccgccga tgacgaaaac gcctcttttg cggtgcgctc
     2821 ctccgctact gcagaagata tgccggacgc ttcctttgcc ggtcagcagg aaaccttcct
     2881 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
     2941 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
     3001 ctccgccggt gttcaacgga tggtgcgctc tgacctcgca tcttctggcg tgatgttctc
     3061 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
     3121 tgaaatggtc gtgcagggcg cggttaaccc ggatgagttt tacgtccata aaccgacact
     3181 tgcagcgaat cgcccggcta tcgtgcgtcg caccatgggg tcgaaaaaaa tccgcatggt
     3241 ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
     3301 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     3361 aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg gccacaccgg
     3421 caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     3481 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     3541 catcggcgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc
     3601 tggcgacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     3661 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     3721 actgggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggtga
     3781 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     3841 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
     3901 gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgaacg aaggcgtggg
     3961 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     4021 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     4081 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     4141 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     4201 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     4261 tggcgcgggc cgctatgttt ccgacagctt ccgcgactgc ttcgcgctgg agtgtgaagc
     4321 agtgaaacga gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     4381 gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg
     4441 tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatt ccgtccaacg ccttgctggc
     4501 agagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata tgacacagct
     4561 ggcgctcggt ctggatcgtg actccggcgt ggtgtctgaa ttgttcgatg agcgcaacga
     4621 tgcggtgaaa gcattgctgt cgatggctat ccgtgccgcg aagaaacagg gcaaatatgt
     4681 cgggatttgc ggtcagggtc cgtccgacca tgaagacttt gccgcatggt tgatggaaga
     4741 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc
     4801 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccgggttt atgtgatccc
     4861 cgaagataaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc gcataatatc
     4921 ttttcttaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt tttcgattac
     4981 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
     5041 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     5101 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     5161 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     5221 tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat cgccgctgta
     5281 ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aatatcccat
     5341 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aggtcttgcg
     5401 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgcataaa
     5461 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     5521 aacacttaat aatttaatgc cgagctgctg gcattcacgc gccacttttt tggggattgt
     5581 ggttccgccg caaagaaaga aacgcagcgc tgacaggtcc gcgggttgtt tctctagtac
     5641 attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac gctgctgctc
     5701 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcgccaat
     5761 taagaatggt gccgttacgc catgcagaaa gcccgttgcg tggccgagtg gcgcaggcat
     5821 cataaagaca tcctgccagg tcagattcag ccgcgcgcaa taagcccgct cactggcgag
     5881 aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
     5941 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc
     6001 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
     6061 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg ggcgcgtttg
     6121 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag
     6181 ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccgattt tcaggcaggc
     6241 aagatagata acggtaaatt cacaccagcc aggcagttga aatgcgatgc gatcgcctga
     6301 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     6361 ataggtgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
     6421 ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct gacgatacgc
     6481 cgcacgacgt tgttcgttga acgttaatgt cactttcatg ttggactctc gaaacagaac
     6541 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
     6601 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
     6661 gtgccgcact ccagacaacc ggcagaatca aaatgaatgt tgccggcgtc atcctgctta
     6721 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
     6781 gccagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
     6841 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     6901 gcaagttgat cagcccaatt ttcttcgcat gtcccatgat cattttgcgt accggctggt
     6961 ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggcgaccatt cgtgggtatt
     7021 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc tgcatatcgc
     7081 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgctg gcggagaaat
     7141 cctcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat gcaatggcta
     7201 aatccatgcc acgaactgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     7261 tcatcacgcc atcgttaacc atctgcggca ccattgccag accgccttct ggcaccatat
     7321 gcgcggaata ttcaagcagt ttgccgccgc taattagtgg gcgaatggcg gggtgttgtt
     7381 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac
     7441 aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg cccatcaggc
     7501 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
     7561 gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca gcgtaatgat
     7621 gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta acgccatcag
     7681 ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca gtgaccttgt
     7741 ttccttcacg aaccagcgca tcgacgcgaa cgcctgggat aaactgcgcg cccgcctgct
     7801 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
     7861 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
     7921 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatgctgca aagcctggaa
     7981 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
     8041 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa
     8101 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt
     8161 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
     8221 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
     8281 tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tgcgccgttg
     8341 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
     8401 agtaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
     8461 attggacgtg aacagccgac ttcagcattc agaaccgccg ccagttcgtg gaccattttc
     8521 aggtcatcct gcgctgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
     8581 tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggagca
     8641 acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
     8701 acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg agcgaacgct
     8761 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
     8821 gcatcattca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
     8881 cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag ggcagcaatg
     8941 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
     9001 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
     9061 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
     9121 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
     9181 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgataatgat
     9241 gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa gtggcgcgct
     9301 ctggctcaag tcaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
     9361 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
     9421 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
     9481 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
     9541 gaccagcaag ccagcctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
     9601 atcgaagccg atcttttcaa ctgctgctgc cagcgctttt gcggtatcga gaggcagtgc
     9661 atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg atagcacgtc
     9721 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
     9781 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
     9841 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
     9901 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
     9961 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
    10021 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
    10081 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
    10141 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
    10201 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
    10261 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
    10321 tttgcaatta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
    10381 gagctgattt acgtcaagaa aggggtagca aggtttacca tcgattcatc gttatatgtc
    10441 gcacatgccg atgatatagt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac
    10501 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
    10561 gaggaaaatc agctactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa
    10621 gaagtcataa aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
    10681 caaacatctt cgttatggga tgcatttgcc tatacgttag ccattcttta ctacgaaaac
    10741 tttaaaaatg cttatcgttc ggagcagggg tatattaaaa aagatgttct gataaaagat
    10801 attctttttt atctgaataa taattatcgc gaaaaaatta ctttagaaca gttatcgaaa
    10861 aaatttcgtg ccagcgtcag ttatatttgc catgaattta ccaaagagta tcgtatttcc
    10921 cctattaact atgttattca acggcgtatg acggaagcga aatggtcact cactaatact
    10981 gaattatcac aggcagagat ctcctggcgt gtgggttatg aaaatgtcga tcactttgcc
    11041 aaactgtttt tgcgccatgt cggttgttcg cccagcgatt accgcaggca atttaaaaac
    11101 tgttttgcgg aacaagaaat cctatctgaa tttcctcaac cggtaagtct tgccgggtaa
    11161 atctggcggc gcagtttgtt aagaagctgc gccgcctgca gattatttgt tctgataatc
    11221 tttcaggatc tgccgacccg caacgtaaat cataatttcg tctgtaccgc cgccgatacg
    11281 ttcacaacgg acatcacgcc agaagcggga gacgcgcgct tcatcggtat agcccagacc
    11341 gcccatgatt tgaatcgcat catcaatgac ttccattgcg gtacgtgcgc aatacagttt
    11401 tgccagcgcc gcgctggtgc gcagtgactg atgctgatcg gcttgccatg ccactttcag
    11461 caccatgttg cgcatgttgt cgatcttaat cgccatcagc gccagttttt cctggatcat
    11521 ctggttatga ccaatgggct taccaaaagc gatacgttgg ttggcatagc gggcggcatc
    11581 ttcaaaggcg cattcggcaa agccggtgct gcgcgcggcg ttgatcaggc gctccatctc
    11641 aaagttgtac atcacattga ggaaacccat tccttcttcg cccaccatgt cgctctcttc
    11701 aacttccaca ttgtcgagat agacttcgca ggtgctgagc atatgccagc cgattttatg
    11761 cagcgggtta atcttaatgc cgggcttact ggagtcgacc caccacaggg tgaaggcttt
    11821 tttgggatct ttcggttgcg gatcgcgcgc cagcaccagc atatacgggt actctttcgc
    11881 accggtaata aaggtttttt gtccgttgat gtagaccttg ccgtttttac gcgtgtaagt
    11941 ggtagtggca ctgttgttat ctgagccagc acctggttcc gtcaacgcca gggcataggc
    12001 gggatcaccc gtttccaggg tgctctctgc cgttttacgt agctgctctg cagaaccgaa
    12061 acggcgcata ctgtgaatac attgaccgtt ggtaattaaa aatgccggag caccgcattt
    12121 tgacacttcc atcagcgcca gcatttgggt gacgtaatcc gcagggatac caccaaattc
    12181 ttccggcacg ccaagcatgg aaataccgtt atccgccagc gcccgcataa actcacgcgg
    12241 atatgtcccg ttttgatcgc aggtgcggaa atactcttcc ggaaagttag tcgtaatcag
    12301 ttcgcgaata ctggccagca gcagttcttg ttcttcagtt aaagaaaaat ccatcataat
    12361 actcctgtta atgagccgct tcaggcagga caaaacccat cgccgcatcg ataaataatc
    12421 tttcgtccat cagtttgagt tctggcgaaa tcactggggt gaagtccatt ttgtcgagaa
    12481 tatctttttg taaatcgacg ccaggggcga tttcgattaa atgcaggccg tcttctttca
    12541 gcgtgaatac agcacgttcg gtgatgtaac gaacatccag tccccgttcg agcgcgattt
    12601 ttccgctaaa agtaatttcc ggtagttccc gaataaattt atttacccgt ccttcctgga
    12661 cgatatgcaa tttgccatcg gcaatttctg tttttaaact gcccgcagtt aacgtgccgc
    12721 agaaaataat tttcttcgag gtggcactga tatcaataaa tccaccggta cccatgattt
    12781 tgccattgaa tttatgcacg ccgacgttac cgtgctggtc gacttcagca aaactcaaat
    12841 aacaaacatc cagaccacca ccgtgataaa aatcaaactg ggacgtcata tccagaatgg
    12901 cgcgggtatt cacgttcgcg ccaaaggcga taccctgtga agtaattccg ccaatcggac
    12961 ctgtttctac cgtcagaata aagtcatcag cacaaccttc ttcccgggcg accaggccaa
    13021 taccgtcagc aataccgacg ccgacattcc ccaccgcgcc tttacgcatt tcgaataatg
    13081 cacgccgcgc aactaattta cgttggttta ggggcaggct aagtttggtg ctgtcatcaa
    13141 gggtgaagtc accagaaata aaacggttaa ccggcgcacc gccatacagt tggctttgat
    13201 ccggatcgac caccacaata tccaccagat aacccggaat acggacagat ttaggatgca
    13261 gcgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg ttattgtgca
    13321 ccgcctgggc aataaccaat gcgtcgagat acatcacctc atcttcaaaa gtggcgtagc
    13381 cttcactgtc gcaggtggta gcgcgaatga aggcgatatc tggcgcaatc gctttgtaat
    13441 agagatattc tttgttatca aactcgacca gtttaatcag gtcttctttc gtgacttcat
    13501 tcagtttgcc gccttgctgg cgtggatcga caaatgtccc gatgccaata tcactaataa
    13561 taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct tgtggatagt
    13621 tataagcaat aattttattt tgttctgcga gttcagaaat acgcggcgat tgtccccagt
    13681 gaccacataa tgcccatttc accagacctt cttgcgccag aggactaata ccacggtcgg
    13741 cgcgatcgcc aagccctgtc ggactaataa tcgataaatt acgtggcgtt tgagcctgtt
    13801 tatatttatc agcaagagct gtaattaacg tggtggcttc cagaataccg ccgccagcgc
    13861 ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt gccgacagga
    13921 ccggcactcg tccattaata cgaggtggtt ttacaggttt cattgttaat tcctgattca
    13981 gatgtgattt tccctacgcc agat
//
LOCUS       DAMZII010000125        13771 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZII010000125 DAMZII010000000
VERSION     DAMZII010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.125"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(164..1618)
                     /gene="amn"
                     /locus_tag="POA77_003722"
     CDS             complement(164..1618)
                     /gene="amn"
                     /locus_tag="POA77_003722"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDI6367089.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1720..3036)
                     /gene="shiA"
                     /locus_tag="POA77_003723"
     CDS             complement(1720..3036)
                     /gene="shiA"
                     /locus_tag="POA77_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDI6367090.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3351..4403
                     /locus_tag="POA77_003724"
     CDS             3351..4403
                     /locus_tag="POA77_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDI6367091.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4665..12648)
                     /locus_tag="POA77_003725"
                     /pseudo
     CDS             complement(4665..12648)
                     /locus_tag="POA77_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT         3423 a         3254 c         3345 g         3749 t
ORIGIN      
        1 tgttttgaaa gcatacttgc ttttaatatg tggaaatcat taagacgata ataaaacacg
       61 tatccgccca aggaagcaaa cagcccacga ttattgcagg ctatttgcca ggtgtcttac
      121 ttactccttc acatctattt ctgataagaa ggttcagaac ttattatcgg aacggcggct
      181 cattaaaggt tcgtaattta cgcgaatgca gtcggtcgcc ttctgcgcgc agcaaatcga
      241 tcgcccgaat gccaatttgc agatgttcgg aaatagcgcc ttcataaaaa cggttagcct
      301 ggccgggaag tttaatctcg ccatgcaacg gtttatctga aacacacagt agtgtcccgt
      361 atggcacgcg gaaacgatat ccttgcgcgg caatggttgc actttccata tcaattgcta
      421 cggcccggct taagttaaaa cgaagtgcag aagctgagta acgtaattcc cagttcctgt
      481 catctgtggt taccacagta ccagtacgta gccgctgttt gacttcctca ccgggcctgc
      541 cgctcaccag cttggtggcg tcataaagcg cacgttgcac ttcagcaatg ctcggaatag
      601 gaatatcggg cggcagaacc gcatcaagaa cgtggtcatc gcgtaaataa gcgtgtgcaa
      661 gtacataatc gccaatggcc tgactttcac gtaatccgcc acagtgacca atcatcaacc
      721 agacatccgg gcgtagcact gccagatgat cgcagatggt tttagcattt gacggtccca
      781 cgccaatatt caccagagta ataccctgac catcggcggt aattaaatgc catgctggca
      841 tctgatgttt tttccatgca aggtcggaaa tggcttcttc tggcgcttcg gtttcggcgg
      901 tgatccagtt cccgccagca caagaaaggg caatgtaggg actatcagga tcgaggatct
      961 ggctgcatcc ccaacgaacg aattcatcca cataacgtgt gtagttggta aacaagacga
     1021 acggctgaaa atgttcaact ggcgtaccgg tataatggcg caaccgcgcg agggaaaaat
     1081 cgacgcggcg cgcatcaaaa tgcgatagcg gggagaattc agttggatga taaatgccgt
     1141 ctgcagtttc atcgccaatt tgcgccagtt ctgttgtcgg gaagtagcga gttaacccag
     1201 cgctcattga gcgatcaagt gtcaattcag agccatcgat gacataagga taggggattt
     1261 catgctgcga gggttgcacc gagatatgcg caccataatc ctgatacagc aacgtaagtt
     1321 gttcattgag atacgaacga aagagagtag ggcgagtaat cgtggtggtg tagctgcctg
     1381 cgtgagtaaa acgaccaaat gcgcgcgttt tggggggatt ggttgtgcta ccgtcccagg
     1441 ttacggtcag tgatggatag acaaaaagac cttgtttgcg ggcgttttca tcaggtaatt
     1501 cgccacttgt aatatagttg ccaatggcgt tgcgtaatgc gactacagat tgctcataca
     1561 gcgcgtcgag tttatccagt gcctgagctg gggtcagacc ggagccctta ttattcatgt
     1621 gtgtctcctg ttccatacaa ttaccgcaca tccgatagta tgtcacagga atgtgaaaca
     1681 aaagtgtaag gcgaagatcg gatgatcttc gccagactat caagcgcgtt gattgtcttt
     1741 catcaacaaa gcggtcattg cggaaatcag acatccagcc agcaaataaa tggcgacgct
     1801 atgccagttc ccggcaaagt aagtgatgag tgcagcggca ataaaaggtg taaatccacc
     1861 gccaaccaca ctggcgacct gataaccgac tccagcacca ctatagcgat aactggcacc
     1921 aaacatttcg gtaaacatcg gttgttgcac acacaccacc atgtcatgcg caatgtttgc
     1981 cagcattatg gagaagaaaa ctatccagaa aatagattgt gcttcaagcg ccataaagaa
     2041 aggaaatgcg ctcaacgttc cgattaacgc acctgtgata taaaccctgc ggcgaccaaa
     2101 acgatcggca agccaggcaa aacagggaat tgtcaagcag cttaatccac ctaccagcaa
     2161 accaatattg aggaaaagtt cgcgcggtaa ccccatattc tgggttgaat aattaagtgc
     2221 aaaggcagta acgatgtaca tcgtcagcaa ttcgcacagt cgtagcgcaa taatcttcag
     2281 gaaagcaccg ggatgtcgta acagcgcttc gataaccggg atgcgttttt tcgcggcagc
     2341 ttgataatgt tgctgttgtt caaattccgc ggactcctcc atgccattgc gcacccacaa
     2401 tgctcccagt accagtacga tgctaaacag gaaaggaatg cgccagcccc agcttaaaaa
     2461 ctgttcgtca gtcgtcatca tactgatcaa tgaaaccagt ccggttgaaa gcagtaaacc
     2521 tacaccgtag ccaacttgta caccgctact gtaaaaggct tttttatttt tcggtgcact
     2581 ttcaacggaa agcaacgccg cgcctcccca ttcgccgccg acggcaaatc cctgaatggc
     2641 acgcagtgtc accagcaaaa taggtgccca ccacccaatg gtcgagaatg aaggaagaat
     2701 accaatcaag gctgtcgcga tgcccatcat ccagacggtc agcattaaca tgcgcttacg
     2761 tcccagtcgg tcgccaaagt gaccgaaaat gacaccgccg agcggacgga aaaggaatcc
     2821 gacgccaaag gtggcaaatg cggcgagcgt tcccatcgcc gggcttactt gcgggaaaaa
     2881 ctcgcgatta aacaccagtg cggcggtgat gccatagagt aaaaaatcat accagtcgac
     2941 gacggcacca gcgaagctgc ctaacgcagc tcgtcgggcg cgacttaacg aaagcgtccc
     3001 ttcatcggga cgagtggaga tgagcgtgga gtccataatg atcctgtttg tactgtcttt
     3061 ttataattag tatggaaaag gtaactcaca ttgccgtcga tctataaacg aaccttatga
     3121 gactacctcg acagagcgca tctgcaaatg tttttatggc tgattagcgg caacatgaaa
     3181 aattcgatgg aagatgaaag cgcaggagtt gctggtgttg tttaaggatt aagcagagga
     3241 cccacaggtt agagatatga aacataaaag tccgcatatg cacaaaatgg caccctgatg
     3301 agtctgatag actgcattgt attataacca gtagggaggc gaggttaggt atgtttttag
     3361 catcattgtt gagacgtatt gcatttagtt actacgatta taaagcttat aatttcaata
     3421 ttgaaaaaac agactttgtt gtcatccata ttcccgacca gattggcgat gctatggcca
     3481 tctttcctgt tattcgggcg cttgaattgc ataaaattaa gcatctttta attgttacgt
     3541 cgacaattaa tttagaagtg tttaatgcac ttaaacttga aaagactaaa ttaacattag
     3601 tcacaatgac tatgcaggat cacgcaacat taaaagaaat aaaagattta gcaaagaaca
     3661 taacacagca atacggtacg ccggatcttt gtatagaggc gatgcgtaaa aagaacctga
     3721 aaacgatgat atttatcagt cagttgaaag caaaaacgaa ttttcaggtt gttggtttaa
     3781 ccatgaagtg ctactcccct ttgtgcaaga acgcgtcccg gatggatcag aatctccggg
     3841 ctcccgtacc catgacatgg gcatttatga tgcgtgaggc gggttttcca gcagtcaggc
     3901 caatatatga attgccacta agtgaggatg tactcgatga ggtgcgcgag gaaatgcgat
     3961 cgttaggatc ttacattgcg cttaatttag aaggtagctc gcaggaacgt acattttcat
     4021 tatcgattgc agaaaatcta atagcaaaaa ttcaaagtga aacagatata ccaatagtga
     4081 tcgttcatgg accaaaaggg gaagataaag ccagggtatt agtggattgt tataataatg
     4141 tctaccgttt atccttatca ccctcgatta aacgttcagc agcaatcata aaagatgctt
     4201 atatcgcaat aactcctgac acctcaatat tacatatggc aagtgcctat aatactcccg
     4261 ttgttgcaat ttatgctgat tacaaaacgc gatggcccgc aatggccgat gtttcggagt
     4321 cagtcgtcgt tgggcaaaaa attgacaata taagtctgga tgaattcgca aaggcattaa
     4381 aaagtgtttt ggcgagaata tgagacagta cttatgcata gtaatgccga tctgttatga
     4441 acggtggtgt catcaatact ctggggctgg tcaatcatca cagaaaacca gcctttggat
     4501 caaagctgta ctcaccggat acgatcatag tcacatcgaa cgcgattctt gcggtgcagc
     4561 agttttattt attcattttc tgtgagagaa gggcaagctc tatagaggga atgtaaaaaa
     4621 gagtgctccg aatggagcac tctttaagaa attataacgg aaaatcacag gtttttataa
     4681 catgtcgcat aagcaaaccc aagcttttca aatacgcttt gatcacctgt tgccagggaa
     4741 actacgtatt gttcgcttga gtttgcggga ctagatgacc aatacatatt tgattgaaaa
     4801 ccagcgtcag tcgtgtaatg ccccatatct ccccattcac tgtacagtga cccgatgcct
     4861 cgggaacgac tatggtttaa atgatctgca cggggaagtg tgtagccatt gctactgcaa
     4921 aaattttcag caaggctgta tatcatgaaa gcatcgccgg agttcaccca ccaactcttc
     4981 acacggattt cgtatacata gctagggcct cctgattttg gcgtcgccgt aatcctttcc
     5041 caattgctgc cgacattttt aaatgtcact ttaccggtag catcaacatc gacccaggag
     5101 gcagagcttg aaaactcata atcagccgcc gtttttcctg gggcaaagtt gtcattattc
     5161 aactgataat acgccccggt gaacccctgc gaagggaatg tagttgtcgg taggtcagta
     5221 ccattgactg atactgtacc gctcattatt ttgtcttctg cgcgagtgaa ttgtattgtg
     5281 gtactcagac cagcttgatg aacaccattc aatacaggga tcagcgttgc gataccctct
     5341 ccgccgcctg taatggtggc tttgtaatca ccgttgatat tttgactgta atcaattgcg
     5401 ctaattttca tatagggcac gttagtacct gattgcacaa attccatccc ttcagaaact
     5461 ttgatcggat tgcctgatat atcgtgcaaa acaagatgta gctcagcact atcggtaaag
     5521 tcccctttca gtgaggaccg attgttctta aggacggact gcgaggcgtc tgccgggcct
     5581 gccaccagcg ttatatctac tgtttgcatt ccaccagcgg tcgtgactgc gcttaccgtg
     5641 acgctacctg tcatcgctcc tttcacgctt gttgtcgcga ttccgttttg atcggtcacc
     5701 gctgttaatg acgttaatgt ggcagagccg ctttttaagg cgaagtacac agtgagacct
     5761 tcgatcaggt taccactgcc atcctcaacc gttgccttta aggtacttaa atcactgttg
     5821 gtggcggcga tagtcgatgg atcagcgata aagctagcaa catgtgcggt gcttgagttg
     5881 ccagtgactt tcacggttgt ctgtgtctgt attgttacgc cgttatggaa cgatgcagtc
     5941 accgtatatg taccgacttt attgctggtc agaacgaccg gagcctgacc tgaagagtta
     6001 gttctcactt ttccgccact taacgtcgcc ccttctggcg ttacgctaaa gttgatgacc
     6061 tgaccctcca ctggagtgcc atttgcagag gttagcgttg ccgtcagagt agtgcctgta
     6121 ggggcataga caccgataag cggactggac gccgtgagtg tcagtttttc atcaatagcc
     6181 tccagttgtt tctcaagtga ggctccattc gccagggatg ctttcaccat atacgaaccg
     6241 tttctttcgg gcgtcatgga gacctcggct ataccatgcg tatcagtaga gacaatattt
     6301 tggctgatgg tcatgtgctc tggtggctct gcactgaatg ttacagattc attaagaatc
     6361 gggttgccaa actggtcgtt gacgtgagct ttaactgcta cgtcttgtgc gaccatgacc
     6421 tgaccttcag gtatctccag actggtaatc gttgcagaat taatatctgc ttttgcattc
     6481 agtaatcgcg atatgacttc agtaggttta tctgccagag aggctgaaac tgttttcagt
     6541 ccgacttcgg tgcttgtcac aagaatttca gcgaaaccct gatcatccgt ttcaacgctg
     6601 gtgctgctta tcgtgacgga ggttccgcgg aaattaactt ttatgccttc taccgggttg
     6661 ccgttagcgt cagtgacgct tgcagtcatg gtcgcgccct cggtcgtgct gacgacgaat
     6721 gagtatacag aggttaagga ggctagtgtt gcggtaccag catcggcaat caaagtcacc
     6781 tgtttcgtat cactgacacc ataattgttc acgctaactg tcacggggta ggtaccggat
     6841 tttgtaccgc tcagtgtaac ttcagccttg ccgttgatat cagtaatggc ggaaccgcct
     6901 tgtccgagag tgaagcttgc gctcacgtct gccggtagcg tgaatgtcac cgcctcattg
     6961 gctaacgggt tgccgtttcc atcagtcact gttgcctgaa gtgtggtcat cccaacgtta
     7021 ttggcgataa agttgtcctc ggtaacgtta agattaacct gcgcactgag cgtatccgca
     7081 ataaagttca ccaccaactg ctcactctta ccgccagtca tcgatgctgt aacagtatga
     7141 gcgcctgctt ttgtaccttt cagcgtgact ttcgctttgc cttcagcatc agtaatcgct
     7201 ttaccgccat cgctcagcgt gaagttcgcc ttcacatctt ccggcagagt aaatgttact
     7261 tcagtgttgg ctatcgcatt gccctctgta tcagcgactg ttgctgttag tgtcgtgaga
     7321 tcgttattgt cggcaatcac cggatccttc gaggctgcca gcgtaatttc ggcattcgcg
     7381 acgttcggca cataggtcac tgtttgttgc atcgaatcgc cattttcgag ggttgccgtc
     7441 acttgataaa ccccggcttt tgtagcggta aagcttgcgt aaaaattgcc gtcgtggttg
     7501 gtagtatata tagcgttatt actgggggtc acgccttcac ttggtgatac tctgagggtt
     7561 acctcctgct ggggtacacc gttgccgtaa ttatccttca cctcaatata cagattggta
     7621 gtgtcgcctg cggagactgt atcaaaaagt gcctgtagca aggtgagatg tgccgtagac
     7681 gcatcagcgt tgacattaat tgatgtgcta agcgtggagc taccattttc cagactggcc
     7741 tcaacggtat ccggtctcgc tcctgattct atcgaggagc gggtattggt ataagtgacg
     7801 gtagccttac cctgttcgtt cgttacggct tggccgccat tcgtcatttc ggcagagttt
     7861 gttctgctgg tgaagttcac agtcacacct ttcaccggaa agacattatt atcaacgact
     7921 gtggcggtga tgacgctgcc ggagctgttc tgcggggtac cggcgattat gctgtctggg
     7981 acaggcgtca actcgataat ttttgccgct gctgtgtcgc caataaaatg cacagttttg
     8041 ttgtcgctgg caccattatt agccagtgat gcagtgaccg tctgctcacc aaaggcaacg
     8101 cctgcgagag tggcctgcgc gatgccagac tcattggtat tacttactcc aggggtcagg
     8161 gtgagtcctg aagaggctga gctgaatgtt accggaagat tattcacctc attgtcgaac
     8221 tgatctttaa ccgttgcagt tagcgttgcg ttatcgacgc cattacctgt gatctcatct
     8281 tttgacatct gcaggacaac ctgagccgag gttttgtccg ccacaaacgt taccggctgc
     8341 gaatcactgg tattgttatt acccagcgtt gcggtaaccg tatgcgtacc cgctttttta
     8401 cctttcagcg tgacatgcgc ttccccattg gcttgggtga tggcaatgcc gttattttcg
     8461 agggtaaagt ttgccgcaac gtcctgtggc atggtgaagg tcaccgttat tcccgccacc
     8521 ggatgattcg acggatcttt caccgtcgcc gtcagctgca cgctgtcgct gttattcgtc
     8581 accacctgtt gagcagggat gttcagggtg acctgcgcgt tgctggcatc gggcgcaata
     8641 ttgactgtcg tcgtatagcc attgccgtta agcagtgtgg cttcgactgt atgaacaccc
     8701 aaaaccgtgc ccttaagggt aacttcagca acaccactgt cgttagtatt gctcgtgctc
     8761 tgactaagtt gagtatctgc cgggttggta ctgaaggtga ccggtaggtc ttttactgcg
     8821 ttatcaaaag gatctttcac tgttgccgtc agagtcgtct catccacgcc attcccaatg
     8881 atttccgctt tcgatgtttg cagaacgaca actgcgctgt ccttatctgc cacgaaggtg
     8941 accggttgcg catcgctggc attattgtta cccagcgttg cggtaaccgt atgcgtgccc
     9001 gcttttttac ctttgagggt gacatgcgct tcgccattgg cctgagtgat ggcaataccg
     9061 ttattttcaa gggtaaagtt tgccgccacg tcctgaggca tggtgaagtt caccgttatt
     9121 cccgccaccg gatgattcga cggatctttc accgtcgccg tcagctgcac gctgtcgctg
     9181 ttattcgtca ccacctgttg agccgggatg ttcagggtga cctgcgcgtt gctggtatcg
     9241 ggcacaatat tgacgatttt cgtatcgctt ttaccgttaa gcagtatggc ttcggctgta
     9301 tgaacaccca aaaccgtgcc tttaagggta acttcagcaa taccgttctc attagtatta
     9361 agcgccttca gactcagttg cgtgtctgcg ggggaggtgc ggaagactac tgacagattt
     9421 ttaaccacgt tatcaaaagg atctttcact gttgctgtca gagttgtctc atccacgcca
     9481 ttcccaatga tttccgcttt cgatgtttgc agaacgacaa ccgctctgtc tttatccgcc
     9541 acaaacgtca ttggctgtgt atcgctgaca ttgctgttag ccagacgagc cgtgatcata
     9601 tgcgtgcccg ctaacgtgcc ggtcagggag gcgattgcag tcccgttgct atccgtcatg
     9661 ccttttccgc tgttgctaat cgagaaccga cttgcgacgt cgtttggcac agagaaggtg
     9721 atttctttgt cttttaatgg gttgccattt ttatcctgca aggttgcagt catatgtagc
     9781 ggagctgtgt tggtgacggt gatatcacct gaaggcacac tgagggtcag ggcagcagta
     9841 ctttgatcac cgataaaatt cacctgttga ttagcctggg aaccagagct cacagaggcc
     9901 gtaaccctat aatcaccatt tttcaaactg gtcagcgtag ctgtggcgat cccgtcgtgg
     9961 ctattcactt cggtttggct cagtttcgct gctgctgaat taacattgaa agtgaccttg
    10021 acgtcattga gcaggttgcc ttttgcatcc cggactgtcg cggtcattgt ggcgctatca
    10081 ttgccgtcag cgaccacttc atttttcgac ttctgcagat caacctgcgc ggtactcgag
    10141 tcgccgacaa aactgacaat taacgtttgt ttcacgccat tttcaagggt gacttcaacc
    10201 gtgttgtctt cctgcttact acttttcaga tcaaaagtcg ccagaccatt aacatccgtt
    10261 tttgcggtgt tttgattgtt aaagcaagtt gccgacccgc ttaataccgc aaacgtgacg
    10321 gtatgatcat tgattgggtt gcttccttca tcagcgacat tgaccgagac ggtgtttgct
    10381 gcattctcat tggcgagcac accattattg ctggcagata atgtcgcaat ttttgctgac
    10441 tgcgggttgg cgtcgatgat aaatccagcg gtatgcaaat cttcattcca gttttgcatt
    10501 aacagcttcg cagtaagccc actgcctctg gtataggcgg tataggttgc cttatagacg
    10561 ccatctgcgg tttctttcca gtctgtagtg acaccaggtt tcacgttgtc gatgctgact
    10621 gcgttattca gttgctgttt ttgctcctta acaggtttgt cattttcatc tctcagttct
    10681 accgtcacct cgataggatt gccggagaga taacggtctt tatcaatttt aattgacgag
    10741 tgagttcggg atgacgaaat agaaatgata ttcaccacgg cgggggcttt agccgcatcc
    10801 acaccattca gctgtggcat cagcgtcagc gtgccagaca tcgctcctgt ggtcaggatc
    10861 tgggtatagc ttccgtcacc attatctttc cattcagaaa gggtgatgtc ctgaacacct
    10921 tcgtgacgcg tcgagagcac cagcccgaca acaggattac ctgctgcatc atgcgcaata
    10981 aaagtcagtg tggcggttga atgggaatcc gcgttcaatg tttgggtact taacgatacc
    11041 gaggaatctt tctggcttag cgtaggtgcc tgaacgacca ccatgctttg ttcacgattc
    11101 gacaaattgc ctttgacatc ttcagcggtg acttcaatcg gccaggtgtt atcggtttct
    11161 ggcgtactgg tgaaccggta acccggcagg gtaaccagaa tatctttacc cgttgtgacc
    11221 actttgccac cggcagcttc cagtgcggtg gcttcgacgt tatagccttt cagggcatat
    11281 ttggtttgta gcgacgaaac cagtgatttc acttctcctg acttccctgt cacggggtct
    11341 gtcagggtca ggcggaccag ttcttttttg cgatattcca gaacgatgtt gttgttgcga
    11401 tccaccagat cataacggct gcctgcaagg ctacgccgtg cagcgacttc attcgggtca
    11461 agctgtttct gcattgcgct gccaggttgc caggtaaaat cgacggcaaa acgggtgtca
    11521 ttttcgccct gtttaccctg gcgttgctcc tcgctgaagg tcattagcgg gaagggggta
    11581 tagttaagtc cagcggttat ggcatgagga ttactttgcc gatcgtcttt atcgaacagg
    11641 gccacttcat cgccataata ctgttcatag accagtttac cgccaaggta cggccaggcg
    11701 ggtagccagc cttctgcgcg tacatcccag ccattggccg ggcgtgcttc ataatcgttg
    11761 tccagttcag gtgcgctgcg ccagttggtc agtcgcaaat agccgttact gcttaatttt
    11821 agatagtcgc gccagtactc cgcgccaatg ccggcgcggg agtggtaacg gctaagatcg
    11881 tggtcgaaaa agaagttgat gcccgacatc catgtgggag tgaaatgacg ccaacctaag
    11941 ccgttgttaa tctgtgtacg ctcgtcagta cgatggagag tatgctgact gaaaaagaga
    12001 ttatcaggcg tttcatacca cggatggaga aaatcgaact gggagttctt caggctaaaa
    12061 tcttcatcca cgcccagcgt gattcttgcg gtaccgaagc ggcttaacca gtctgtcatt
    12121 gcgcctgaag cctgagaaga ggcccatcca cgcgccatat ttgccgcttg ctcgctgttc
    12181 atatcctcgg cgagcagaga cccgatttgc tgtgaagtac tggctatctg ttgctcaagg
    12241 ttgccgctgc tattacccgg tggcggggtt aaattatttt cactaacttg tgccgggaca
    12301 tccagttcat caccctggcg gacattatca aaacctcgag caaacgtacg aaactggttg
    12361 agtttgcgta actcagccac cgaaataccg aaacgttcgg caacgctttg ggccgatttc
    12421 cagcgctcca agggtgtagg gcaccgtatt ggcatttgca atggcgagtt gtgcaggaac
    12481 tggttgttgg gttgcggcgt ttaccacacc ttgtgctgcc gcagccatag ggaacgcaag
    12541 ttgagttatc aggcagatac ccgcagttaa gcggcgtagt ttaattccca tcccgcataa
    12601 gatttgtcgg tcatttattt cttctccact tctcttcttc gtagccatgc ttaccttccc
    12661 tgaacgagct aacataaatg cgccctttct agcacagcga aaaaatgtaa atcctgatag
    12721 caatatgatt gaaaattaat aacttttcgt taggcagttt tgggtgtgag ttgcaagagg
    12781 ggagactact gaataactca agttttataa tcgaggggaa aatggtgatg gcgttcatag
    12841 caaaacgccc tcaaccataa aggtcgaggg cgcttaagat gttaaaaacc cgctatccgt
    12901 taataaacaa tgttcaacta aggttagtga cattgcgcta agaagcgaat ttttagaatt
    12961 tggctcctct gactgggatc gcccaagcca ataactggct aataaataag ctaaaccttg
    13021 ttgctcttcc tgtcaagacc accataatga ccaccaattt cgaatgagtg tgcgacaaag
    13081 cgtagatgcg atgcttatta ctacagtaaa gtagaacagg ctgaggcgtc gttatgattg
    13141 gatggtaacc ctgcgtatga aacttggatg gctagcactt tatccgccta ctgagtggcg
    13201 ggatgcttgc ttgttaagca taggaaaaac tctgcattta ctgtcttgaa aaaatcaatg
    13261 tacataggac gtgccccaca gacatctcag ggcaatgttg atgccgtccg ctcttacaca
    13321 aatcgttcag agttggagta tgcatttcat gtcggatatc ctgtgtgaat ggcgatgttt
    13381 tttggcactg aatttagtga atattttagc gggcgtgttg gtagctctac gatttttgga
    13441 taacctcttt tcgggagctt tttagacatt tacactttta aagccttcta atgataagtt
    13501 tgtgtctgtt ttatctcttt gtggtagaat ataatgctta ttatttggtg gtttactgag
    13561 actcatcgcc gataggacat tatgaatact gttactaatt gagctatttt tactaaaaaa
    13621 catatagtta tgtcatggtg cagccaaaat gggctggtaa gttgccactt taagaaagga
    13681 aaatgttctg aaaaaagagt aaactgccag ccacaaagca cctcaataga gactgaaccg
    13741 agaacccagc cacatgctac aaaacaaccc t
//
LOCUS       DAMZII010000126        13612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZII010000126 DAMZII010000000
VERSION     DAMZII010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.126"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            7..837
                     /locus_tag="POA77_003726"
     CDS             7..837
                     /locus_tag="POA77_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDI6367092.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(978..1751)
                     /gene="uxuR"
                     /locus_tag="POA77_003727"
     CDS             complement(978..1751)
                     /gene="uxuR"
                     /locus_tag="POA77_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDI6367093.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1966..3426)
                     /locus_tag="POA77_003728"
     CDS             complement(1966..3426)
                     /locus_tag="POA77_003728"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDI6367094.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3507..4691)
                     /gene="uxuA"
                     /locus_tag="POA77_003729"
     CDS             complement(3507..4691)
                     /gene="uxuA"
                     /locus_tag="POA77_003729"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI6367095.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5031..6374
                     /gene="gntP"
                     /locus_tag="POA77_003730"
     CDS             5031..6374
                     /gene="gntP"
                     /locus_tag="POA77_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDI6367096.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6618..7520)
                     /gene="fimH"
                     /locus_tag="POA77_003731"
     CDS             complement(6618..7520)
                     /gene="fimH"
                     /locus_tag="POA77_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDI6367097.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7540..8043)
                     /gene="fimG"
                     /locus_tag="POA77_003732"
     CDS             complement(7540..8043)
                     /gene="fimG"
                     /locus_tag="POA77_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDI6367098.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNNGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8056..8586)
                     /gene="fimF"
                     /locus_tag="POA77_003733"
     CDS             complement(8056..8586)
                     /gene="fimF"
                     /locus_tag="POA77_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDI6367099.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8596..11232)
                     /gene="fimD"
                     /locus_tag="POA77_003734"
     CDS             complement(8596..11232)
                     /gene="fimD"
                     /locus_tag="POA77_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDI6367100.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGVQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11299..12024)
                     /gene="fimC"
                     /locus_tag="POA77_003735"
     CDS             complement(11299..12024)
                     /gene="fimC"
                     /locus_tag="POA77_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDI6367101.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12061..12600)
                     /locus_tag="POA77_003736"
     CDS             complement(12061..12600)
                     /locus_tag="POA77_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6367102.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12665..13213)
                     /gene="fimA"
                     /locus_tag="POA77_003737"
     CDS             complement(12665..13213)
                     /gene="fimA"
                     /locus_tag="POA77_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6367103.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3314 a         3495 c         3294 g         3509 t
ORIGIN      
        1 actattatgt ctatgccatt aagcaatgcc cttcagagtc aaattattac agataatcac
       61 ttccttcatc acccaaaagt cgacaatgag ttaacacgta aatatgaacg agctcgcctg
      121 gatacggaaa atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga
      181 aaaagcgttg tcgaaattag aaaacttgat attagtaaag agtcctggcc atttaattat
      241 gtgacagaag catgcagaga atccgatggt attacaacaa caggacgtat gctctatcga
      301 aacttgaaaa tcacctcagc tttagacgaa atatatggtg gtatatgtaa aaaggcgcat
      361 gctgctacag aattagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat
      421 cctgttgagg actatacagt tcatgaaata acattaggcc ctggttgcaa tgttccaggt
      481 tacgccggaa ccacgatagg atatatttca acccttccag tttctcaggc aaaaagatgg
      541 accaatgagc agccaagaat tgatatctat attgatcaga taataactgt ctcaggggta
      601 gcaaattctt ccggattcgc ccttgccgca ttacttaatg ctaatattgg aatgggaaat
      661 gacccaataa ttggtataga agcctatccc ggtaccgcgg agatccatgc aaaaatggga
      721 tataacgtta tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca
      781 tctcttccag aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca
      841 ctgagaagaa tgagtactaa tattcaggtt atacagatat ttaaacaata ccctgcaatt
      901 tacaaagtaa tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt
      961 gcgcttatta taataagtca ggcgtcgact ttatccagcg gccatgaatc aaacagatag
     1021 ccgtcaaaat aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc
     1081 agatgctgcc acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg
     1141 atctgtttgt gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc
     1201 aactggatcc acattgggtt gttttcccgc cactgccagg actgacggaa cagctccacc
     1261 agcatgctgt tatgcgttgc ttcggcaata gcgagatgga actgcatgtc accgctttcg
     1321 ctgctgcccg gcgcactgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt
     1381 ttgacgatat cttcgcgggt agcctgcagg gcggcaaact cggcgatgtt actttccagt
     1441 aactgacgcg cctgtaacag ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga
     1501 ctgtctgtgt tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact
     1561 tccaccagcc ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca
     1621 tcaagcattt ctgcaatttc acgctccggc ggcagccgtt cgccagggtt gtacggcgtc
     1681 tttatgatca gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg
     1741 gcagatttca tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc
     1801 gtgaacgcct tatccggcct acaacgggca attttacggc ccccgttaat ctcaaactat
     1861 accattagca ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca
     1921 aatatcagtt tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac
     1981 acactcgcgc gcaccgcgtt cgcacagctg ctgatatgcc gccgtcactg cgccaacaaa
     2041 gtcggcattc tgcggcagat catcggcaaa aataccgctc aggcccagca gcgctttcac
     2101 gcggtctgcg ccctgatact gcgcgttgat cttctggaac tccgccagca tcgggtcgac
     2161 cacgtcaatg gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac
     2221 gcccagcgcc aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag
     2281 cagacgctgc ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag
     2341 agacgggttg ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ctgtaccttc
     2401 cggcatcgac agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata
     2461 agccgggtta gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc
     2521 cagaaaagag tggctgccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc
     2581 tgcaacgaac tgtgcgccca ctttatccca gtccgggcga ccattaacga agttatcttc
     2641 aatcacccac tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg
     2701 gtcagcaatt tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt
     2761 gcacgggaag gtgacatttt cttcaatcca tgccgccagc tgcgggtcac gcgcctgagc
     2821 cagccccagt accgcgacct tcgccacatg tccgttttca cgcacgttat cgcaggacat
     2881 caccgtaaac gctttcaacc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc
     2941 aatcgcggac ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag
     3001 atccagttga ccgcttgccg catcagcgca gtagcctttt tccgtgaccg ttagagagac
     3061 aatcgccgtt tgcggacgcg ccatcgcgtt gagaatacct tcgcagccat cgatttccgg
     3121 atgcagcgct tctttcatcg aaccgataat tttcagctcg gtgctctctg cgcctttttc
     3181 cgctacggtg tacagcagtt gctgtttttt caggttttcg atcagcacgc ggtcgttgcc
     3241 tggcatcagg ttaacttcgc agatgcccca gtcgctgtcg gtgctttcca gcagatgatg
     3301 ggtatacagc gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga
     3361 ttccagacga gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt
     3421 agtcatcgta aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc
     3481 acggcgcgca gccatgccgg tggatattaa cggctaaaga aagcgcgctg gatcgccagt
     3541 tcgacaccgc gaacttcggc caggcctttc agacgaccaa ttgcggagta acctgggttg
     3601 gttttcttct tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg
     3661 tcttctttgc cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg
     3721 tacatatcaa cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca
     3781 cgcatggtgg agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca
     3841 accagatcgt tgtcagcacg cacgccgtag gaaccggtgc acatggtaaa accgtttgcc
     3901 atgctgttta cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc
     3961 aggccgagga tcgggcgcgg cggatcgtcc gggtgaacag ccatacgcac gccgacttct
     4021 tcagcaactg gaataatcgc tttcaggaag acggcaaagt tttcgcgcag cttcgctttg
     4081 tcgatatctt tgtacagctc caggtgttta cggaactggt cgagggtgta gccttcttcc
     4141 gcgcccggaa gaccagcaat gatgttacgg gtcagacgcg ctttgtcttc gtcgctcata
     4201 gtggcgaagc gaacagcagc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct
     4261 ggacgtttca ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagcgct
     4321 ttggagccgt ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg
     4381 aagttgtagc acacggtgcg aatgccgcac tgcgccaggt tgcgcagagt ctgctgatag
     4441 ttagcgatcc actgctcata gttgccagtg tgggttttga tatcttcgtg aattggtacg
     4501 ctttctacga cagaccacac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc
     4561 tcttctacgg accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt
     4621 gcgcccgcct gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag
     4681 gtctgttcca tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac
     4741 cctggagttg tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgtatcgga
     4801 tgagatggcg tataagttct accttttggt aagacatgtc aacccgaatt ctgaaattgg
     4861 ttaaccacat cacaagaatt tcactctcat ggttgttgct gcaaattgca gcaaagggta
     4921 atcgatggtt attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca
     4981 taacatgtcg cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc
     5041 ttaacattct ctgggtggta ttcggcattg gcctgatgct ggtactgaat ttgaagttca
     5101 aaatcaattc aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcgggca
     5161 tggatttgat gtcgctgctg cacactatga aagcgggctt cggcaacacg ctgggggaac
     5221 tggctatcat cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg
     5281 ctcaccagat agcgcatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg
     5341 tgattatcat cggcctgatt tttggtctgg cgatgttcta tgaagtggcc tttatcatgt
     5401 tagcgccgct ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga
     5461 tcccggcagt agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg
     5521 tggcgctggt gaatgcttat ggcgcggata tggggatggt ttatatctat ggcgtactgg
     5581 tgacgatccc aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg
     5641 agcgcccaac gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct
     5701 ctttcggcgt ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca
     5761 tcgccaatat ctggctggta aaagataccc ctgcctggga agtggttaac tttatcggtt
     5821 cctcgccgat tgcaatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcac
     5881 gtggtcatga catgcagtgg gtgatgaacg cttttgaaag cgcggtgaag agcattgcaa
     5941 tggtgattct gatcatcggt gcgggtggcg tgctgaagca gaccatcatc gacaccggca
     6001 ttggcgacac catcggcatg ttgatgtccc acggcaatat ctcgccctac atcatggcat
     6061 ggctgatcac tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg
     6121 ccgccgggat tatcagtgct gcaatccttg atccagcaac tggtcagctg gttggcgtga
     6181 atccggcgct gctggtactg gcgacggctg cgggttccaa caccctcacc cacattaacg
     6241 atgcatcttt ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa
     6301 cctggggtct actggagctg gtcaactccg tggttgggct tattattgtg ctgattatta
     6361 gcatggtagc gtaaaaggac accagagcct gccaatggca ggctcagact gatgacaaag
     6421 tctaaaaatg tgcccagaca gtcccctcgc ccctccgggg tgagggttag ggtgagggga
     6481 aaagacccgc aaagtgcgct ttgtttatgc cagatgcgac gctgacgcgt cttatctggc
     6541 ctacaaaggg ctaacgtgca ggtttttagc ttcaggtaat atttgcgtac ctgcattagc
     6601 aatgccctgt gatttcttta ttgataaaca aaagtcacgc caataatcga ttgcacattc
     6661 cctgcagtca cctgccctcc ggtacgagcg taatttgccg ttaatcccag acttaccgcc
     6721 gaagtcccta ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc
     6781 gtcaactgta cgccgacgcc ctgcgctggt gaaaacgagg cggtattggt gaaaatcgag
     6841 ttgcccgcat ctgcggttgt gccggagagg taatatccca ggttttggct tttcgcacaa
     6901 taaacggtaa gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca
     6961 cgagcagaaa catcacagcc gccagtaggc accaccacat cattattggc gtaaatattc
     7021 cacacaaact ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca
     7081 attaatgagc cagctttaat cgccaccccg cccgcactgc tcacaggcgt caaataaagc
     7141 gccaccggcc acggcttatc cgttctcgaa ttataaacaa cgcgcggcgt ttcgctggtg
     7201 gtcggaaatg gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg
     7261 ccgccataag ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg
     7321 ttatggcaaa agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca
     7381 ggcgcaaggt ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg
     7441 gcggttttac aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac
     7501 agggtaataa ctcgtttcat tacaatcatc tcttcgggtt cagctgtagg tataggtgat
     7561 gctaatcact gcctgaatgg ttccctgagt ggctccgcca tttactgtca atgctctgac
     7621 ctgtaacggg aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc
     7681 attattcaat gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc
     7741 ctggttttta taatatccgg tactgtcggc tgccccgctg aagctggcag tgaccctcga
     7801 cgttcccacc ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc
     7861 ggcagacata agactgaaag aataaagatc gccgagatca accgtggcat tggtggtgga
     7921 aactgtgcac ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg
     7981 tatcgtcgca cttgcgagcg ccaatattgc cgccaataaa tacccacgtt tgcgccattt
     8041 catgagcatc tccagttact gatattcaag agtgaaggta gccgtggcat tgatatgccc
     8101 cgcagtgact ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt
     8161 acccggcgtc agggtcgtcc acgaaatcgc ggacgatgga gcattaaggg gtatttgatt
     8221 ttgctgctca ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag
     8281 tgcaagcagg ttggcattgt ggctatctgc aacgccagta aacccaacct ttacggcaga
     8341 aacggcgtta ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc
     8401 aatgttgtta aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc
     8461 agcggccaca ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc
     8521 agccaaagcg tgactcaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt
     8581 tctcatcacg cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact
     8641 ctctggtggc agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac
     8701 ctgaactttt cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat
     8761 gccgctactc tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt
     8821 gtgggttagc gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat
     8881 cgccccacga gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccag
     8941 ggtattggta tccagcgcca ctctgttttc ccgatattca gtggcataag gcagcacggc
     9001 ataaccacgc cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc
     9061 aggcgcttta acaagcacca ctgtttcgtt taacggctgc cccagcgtta cgccattggc
     9121 atgagccaat accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct
     9181 gtaaccgata ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtgct
     9241 tccgctatta ccatcgcctc ccccggcata gccggtttgc acgctatagc tgaggttgtt
     9301 gtcttccagc aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg
     9361 tgacatgctg tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca
     9421 gtggctgaaa ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc
     9481 gtttttcgtc aggctatagc tgagcgtcca gttgatatct tcgaatgcag tatttaaacc
     9541 agcctggaat tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa
     9601 atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt
     9661 gttataagcg aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccgtcctg
     9721 tgtttcgatg ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc
     9781 gctggtcgaa taacggtaac ccactaactg aatattcgtg cctgactcat tgagcgattt
     9841 gttatagaga aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt
     9901 agcctgcgtc atatccacag acagagcgcc cagtgccccc atgtttttcc cgataccgaa
     9961 attaaaagca cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg
    10021 aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact
    10081 acggtattct cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac
    10141 tgacgaatag ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg
    10201 caagtcacca ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac
    10261 cgtactatta taaatgtcat acccattttg tttaatagtg acctgtgcag taccacgagc
    10321 aataccgtgg atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga
    10381 ggccaattgt acgccgcgaa agttaatacc atcgaaaata tcgccctgag tataaccatc
    10441 acccagcgtc agccgggaac gtaacggtat tatgtctcgc tcaagccagg tattgatatg
    10501 ctgccattta tttttgctac ctgatgatct gtcgctactg ttataactcc aggtggtatt
    10561 gtcgcgtaaa cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg
    10621 gctgttaccc ccaatccgat tctgtacact atttccgctg aaattataat tgagcaatcc
    10681 ggcattaata ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat
    10741 aaatgcctga gggatcgtca ggttgagtcg ctgctgacca acatctaaat gcgcagtagc
    10801 gtcatggatc attgaggtta atggcacgca ggcatcatcc gccagcagat tcataccgga
    10861 gacagaagcc gtattcagcc ccatactggc aagctgcgcg cgtgtcaggc agggaacaat
    10921 cccttgttca ctgtcgcccg tattaaatgt gacatcacgc gttgccatat aaccattatt
    10981 caaatagata tcgacacgat acgtccctgg cggtaattct tgcccatttt caaaacgcga
    11041 taaatcagcc acagcctggg gatcatccgc taaaaaacgc gggttaaaat agagttcggc
    11101 agatgacaaa ggtgcctgtg cggcaaaagc acaggcaaca aagagccgga caaaaaaacc
    11161 agccaaacga tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt
    11221 cagatatgac attattcctc cggtttaaaa tcacccggca gtcaattctt tttattcagg
    11281 cgaaaaattc cccctgcgtt attccattac gcccgtcatt ttgggggtaa gtgcgccata
    11341 atcatttatt gttcggtaag taatattgct tcctgcatca gaaggcaatt taaccgtgct
    11401 ttcgcccatt ggaggcacca atgcattttc aagaacccgg gttccggcat tcaactctgt
    11461 taccgtcagg taatagggtg tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa
    11521 tcttaatttt tctgcggcct gatcgggtgg caacgctaat ttagctgggc gatagtacag
    11581 tttaatgcgg ctgataattg cgagctgtag cgtattctca gtcaattttg atttatccat
    11641 tgacggaatc gctttaacat tcatccagaa taaactttcc cggtcctgtg gcaattggtt
    11701 atttgttgca tcaagaatac gcaaggtatt ctcttttttt cccttcatcg caaacagagg
    11761 aggcgtcacg ataaaacgac catcctttac accatcggca ttttccaccc atgattgaat
    11821 taaataggta ctattttcat cattatttgt cacggcaagt tgcacttgtt tttgccctgc
    11881 cggataaatt acgcgagtcg cacctaaggc cactcccgct tcagcgcgtc cggcaaccat
    11941 cattgccatg aacatcagga tacctgccag caagcagaat gttatttcct gcgatttcct
    12001 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tgctgaacaa
    12061 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
    12121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
    12181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
    12241 gccaatattg gtggctatcc ctggcccctc tcccacggaa agcacatccg gatttttacc
    12301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
    12361 taaatgaata acaaaaggca ccggtgcgct atcttcccct accgcatgaa accggttact
    12421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
    12481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
    12541 tccggccaga gcaaacattg gcagcaacga cgctaataga aataatctct tccttttcat
    12601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtccc tgaacctggg
    12661 taggttattg atactgaacc ttgaaggtcg catccgcatt agcagcaccc ggggttgcgg
    12721 caccggttgc aaaataacgc gcctggaacg gaatggtgtt ggttccgtta ttcagggttg
    12781 tttctgaact aaatgtcgca ccgtccagcg ccagcgcagc acccgttctg tccaggatct
    12841 gcacaccaac gtttgttgcg ctacccgcag ctgaactctg cagagccaga acgttggtat
    12901 gacccgcatc aatcgccgta cctaaaaagg caacagcggc tttagatgca acattggtat
    12961 cgcaatcatt cagctgaatg ttaaaaccga cagcagaact ggtcgctcct tcctgtgcca
    13021 gcgatgcggt acgaacctgt cctaactgaa cggtttgatc aacagagcct gcatcaactg
    13081 cgcaagcggc gttaacaact tcccctttaa agtgaacggt cccaccattt accgtcgtgg
    13141 cagcggccag agccgctgta gaactgaggg acagagccga cagaacaacg attgccagag
    13201 ttttaatttt catgctgctt tcctttcaaa aaactatttc taaatcgaca tgggcagtcg
    13261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
    13321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgga caaacttttt
    13381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagatgt ttatattgca
    13441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
    13501 tacatatagc ggaggtgatg tgaaattaat ttataagaga aataatttac atatcaaaca
    13561 tttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cg
//
LOCUS       DAMZII010000127        13516 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZII010000127 DAMZII010000000
VERSION     DAMZII010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.127"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..977
                     /locus_tag="POA77_003738"
     CDS             <1..977
                     /locus_tag="POA77_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI6367104.1"
                     /translation="ITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFS
                     HLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDDCILDDSN
                     FAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPY
                     VNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVIC
                     TDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEEN
                     IPIDSDTLVTRQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1131..2780)
                     /locus_tag="POA77_003739"
     CDS             complement(1131..2780)
                     /locus_tag="POA77_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDI6367105.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2931..4280)
                     /gene="ghxP"
                     /locus_tag="POA77_003740"
                     /gene_synonym="yjcD"
     CDS             complement(2931..4280)
                     /gene="ghxP"
                     /locus_tag="POA77_003740"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDI6367106.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4827..5291)
                     /gene="soxR"
                     /locus_tag="POA77_003741"
     CDS             complement(4827..5291)
                     /gene="soxR"
                     /locus_tag="POA77_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDI6367107.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5377..5700
                     /gene="soxS"
                     /locus_tag="POA77_003742"
     CDS             5377..5700
                     /gene="soxS"
                     /locus_tag="POA77_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDI6367108.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5703..7289)
                     /gene="pdeC"
                     /locus_tag="POA77_003743"
     CDS             complement(5703..7289)
                     /gene="pdeC"
                     /locus_tag="POA77_003743"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDI6367109.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITYFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7719..8000
                     /locus_tag="POA77_003744"
     CDS             7719..8000
                     /locus_tag="POA77_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDI6367110.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8099..8635)
                     /gene="ssb1"
                     /locus_tag="POA77_003745"
     CDS             complement(8099..8635)
                     /gene="ssb1"
                     /locus_tag="POA77_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDI6367111.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8889..11711
                     /gene="uvrA"
                     /locus_tag="POA77_003746"
     CDS             8889..11711
                     /gene="uvrA"
                     /locus_tag="POA77_003746"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI6367112.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11746..12102)
                     /locus_tag="POA77_003747"
     CDS             complement(11746..12102)
                     /locus_tag="POA77_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI6367113.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12106..12522)
                     /locus_tag="POA77_003748"
     CDS             complement(12106..12522)
                     /locus_tag="POA77_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDI6367114.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12633..13346)
                     /gene="aphA"
                     /locus_tag="POA77_003749"
     CDS             complement(12633..13346)
                     /gene="aphA"
                     /locus_tag="POA77_003749"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDI6367115.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3535 a         3499 c         3278 g         3204 t
ORIGIN      
        1 gaattacgag aagagtttac cttagtaaag aaaaggaagt atccattaag gtggtatatt
       61 ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagaca gtaaatggtg
      121 gttacgattt ttcacacctt agcctgaaag gtatcgtgat taaagatgaa gatttatcca
      181 attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt aatatgtata
      241 aaacgaattt ttattacgcc ataatggaaa aaatactttt tgatgattgt attctcgatg
      301 actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc tccgctatgc
      361 atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc tttcttgact
      421 attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta atagcgccat
      481 atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt aactttgaac
      541 atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata aacttaattg
      601 atagcaaact cttttgtacg tggctaacaa atacattcct cgaaatggtt atatgtaccg
      661 actctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc catttcaact
      721 gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt aattttgatg
      781 aggctagcgt ccagggaatg ggcatttcca tactccgtgg ggaggaaaat atccccattg
      841 atagtgatac cctggtaaca cgacagaaat tctttgaaga agattgtacc tctcatactg
      901 gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact gcagatatta
      961 tgcaacacgc agattgaggt taaatcgaag aacagccaat gaattgactt tacaagagag
     1021 ataacttatt tgttttagaa tcatgaatgt tagcccgagc taacctgtta aaggtgcaaa
     1081 agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc ttactgattt
     1141 tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg cagcgtttcg
     1201 ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga acgcaatgcc
     1261 gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac gtcgttacgt
     1321 ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt aagcagctgg
     1381 ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg gatcgccaca
     1441 atcgccactt ctgccgttgc cgctcgcgca atacgttcct ctttcaattg ttgcgaatga
     1501 tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac aaacagcgag
     1561 aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa gacgttacca
     1621 tccggcaaga gcagcgggat agagagcaca ccggccagag tgatcgcccc acgcacccca
     1681 gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat cggcttcttc
     1741 ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg gaccagcatc
     1801 agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac gttagggtcg
     1861 atttctgccg ccatcagcga cgtctccaga atacccggta gctgcagacc taacagcagg
     1921 aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc acgcaggcgc
     1981 attgccagcg gcgcacggcg catcacaccg gagcgggtga tggtcatccc ggcggcaacc
     2041 gcagcgagga tgccggaaac gccaatatgt tcggcaatca gataagaagc gaatggcagc
     2101 aggaacagca ggacgatctg cgtcgcgggt tcatcaccgc cccagcggct gaggaatcgc
     2161 agcgagcgac cgtacagcca gctaaccaca aaaccggcga gaataccgcc aatggctact
     2221 ttcataaatt cgaccgtcgc accgccgacg gtgaagatca tcgtccccat cgccactgct
     2281 acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc gccctgcaaa
     2341 atgcccatga tttttttcgg gatacgccct tcgccaacaa tcccggagag cgccacagca
     2401 tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat gcccggcacc
     2461 acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc cagcgcgagg
     2521 ccaaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca gccatcagcg
     2581 aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc cacatgcaaa
     2641 ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg aagcgggatc
     2701 tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt cattatcagt
     2761 atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat ctgagaaaga
     2821 cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa tgtgcggaga
     2881 aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga ttagatagcc
     2941 cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc gatgttcaac
     3001 ttgcgccatt cgccggaaac cagacgacca atcaccagag tcgcgaagcc gatcatgatg
     3061 cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc cgccatcgca
     3121 tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag gccaacgtac
     3181 atcagcgccg gagccgttgc ataccccgga acgaggtaag agagcggaga gaggaacaga
     3241 atcagcagga acagcacgcc aacggtgata gccgtcaaac cggttttacc gcccgccgcc
     3301 gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag gccagagaaa
     3361 acgctgctca tggagtcagt ggtcagtgct ttcccaccgt cgatgatctg cccatcttta
     3421 tccagcaggt tcgcctggcc ggcgacggca cggatagttc cggtggcatc aaatactgcc
     3481 gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc cataatgtcc
     3541 aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc gaaaacgccg
     3601 gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat aatggtcagc
     3661 agaatgccac cagggacttt cagtttttcc aggccgatga tcaccgccag acctaccagt
     3721 gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc atcaagcggg
     3781 tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag accgataccg
     3841 atccccgtgc cgtgcgccac accgtgaggc aagttgcgca aaatccagct acggataccc
     3901 gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag cgcgacaggt
     3961 acgctaatat gttgccccag caccaggctg aatgcggtaa atgccgtcag ggagatggcg
     4021 caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc gagtccggca
     4081 accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc caacatacct
     4141 ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc aaccacttcc
     4201 tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga accgccggta
     4261 cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc gtcaagatgc
     4321 gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt gcgacaattt
     4381 gctgcgtcgc aaaaaaagca aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa
     4441 gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg cttttttctc
     4501 ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat cgtcggtgcg
     4561 caagattgca gcgttatcga gccgtatacc gttcaggatt gcgataaaaa atcgtctgag
     4621 atgacaaaaa tattgcggtt aaaaagtata cttttacact ggcgaagaaa cgtcggtgac
     4681 atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat gaggtgtgtt
     4741 gacgtcgggg gaaaccctcc tgtgtaccag cgggatagag agaaagacaa agaccggaaa
     4801 acaaactaaa gcgcccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg
     4861 gtaccttctt ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg
     4921 cagccacaac caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc
     4981 cgatccaact cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta
     5041 tgcccttcgg gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc
     5101 tgagcaattt tgataattgc aacatatcgc aacacatcac gtttatatcg ccgctgattg
     5161 ccgctgttac ggatactggt aatcaaccct ttactttcat agaaatgcag cgccgatacc
     5221 gccacaccgc tgcgtttcgc cacttcgccg ggggttagca gcgctttaat gcggggtaat
     5281 ttcttttcca taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat
     5341 gaattaacga actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag
     5401 gatcttatcg catggattga cgagcatatt gaccagccgc ttaacattga tgtagtcgca
     5461 aaaaaatcag gctattcaaa gtggtacttg caacgaatgt tccgcacggt gacgcatcag
     5521 acgcttggcg attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc
     5581 accgagcgtc cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc
     5641 tcccgcgttt tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtaa
     5701 ttttattgcc cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc
     5761 tgcggcggca tcgccttcgc aaagaaccat ccctggcaat agcgcacgcc gcgtttgcgc
     5821 agccagttaa cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg
     5881 tgcgccagct cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt
     5941 tcaacaaacg atttgtcgat tttcaaaata tcgacattca atgatttaag gttatgcaag
     6001 ttagagtagc caataccaaa atcatcaatt gccacttcgt aacctgcctg gcggaaagcc
     6061 agaataatcg gcgtcatttt gtcaacatca agaaatgcat gttcagtcac ttcaaattta
     6121 atttgctgcg gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt
     6181 gacgtatgaa aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc
     6241 aggtaatcgc ccagatcacg gaagacatta tcaataacat aatcagttat ctgttctatc
     6301 atcccctcct tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca
     6361 ccaagccaac gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt
     6421 ggctggtaat aaagacaaag ttgatgtttt tctagagcgc gttgcaattt gcgtttggga
     6481 gacaaatagt tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata
     6541 ccggcgggca acgcaaaaat aagatgatta taaaaatagg ttataagacg ttgatatgaa
     6601 gtcgcaacaa tggctgcaat tgggcgtttt gtcgaataaa ctgtcgcata taaatagcca
     6661 tttctttgta cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc
     6721 tctttgctta acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc
     6781 ggatcatcag acatcacttc actccagaag agagggttga taaccgccac ataatttccc
     6841 cgctgcatat aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata
     6901 gaaacgttag gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct
     6961 atcagcgatg agcataaaaa atggttatca cgggcataga tcaattcatt aatataaaga
     7021 tagccacgaa taatattcaa cattcgcttt tgatgggctg gagtgcaaac ttgcccctga
     7081 tagcgttcag ctgcatcgct cactaaatct gcctggcgaa tgaccagctc ggatttatcc
     7141 agtgccagtt gagcaaaggt tcggagctga ttattcactt ctgattttgc ccaaaggaag
     7201 gcaatccata gcgaaagaat gatgggaaag agaactaaaa agataatgcc cggcaacgcc
     7261 agtaattggt gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt ttatacccgg
     7321 ccattgcaag gtgaaaaccc tgggtaccga cgtctttaga taaatcttac ggtataaata
     7381 gcacttttgc ctgatttgaa attgatacct ttctaatcgt cacctggatg aaaataattc
     7441 attgatattc aaaattaatt tcctacagga atgcagaggc ggcgggaaga tatgccctta
     7501 atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta atatggctaa
     7561 caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt gtttcatttt
     7621 tgtgttctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa caacgccgcg
     7681 ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc accactggcg
     7741 tggttcttct tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc agcacactca
     7801 acatccgttt tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg
     7861 ttgtttccgc ctgttggttc gcaatggggt tgcttggcat cacaatggcg gacatcaccg
     7921 ctatctggca caacatcgag tcggtgatga tagaagagat gaatcagaca ccgccacaat
     7981 ggccaatgat tttgacctga tacgtagaag cctccataac ggaggcttct tttttatggc
     8041 tggcagatgc tttaatcatc caccttaaaa caatataacc tattgtttta atgacaaatc
     8101 agaacggaat gtcatcatca aagtccatcg gcggctcgtt agacggcgct gccggagcgg
     8161 actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccaccctgc ggctgctgtg
     8221 gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgcc ggagcgccac
     8281 caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg acttctgtgg
     8341 tgtagcgatc ctgaccggat tgatcggtcc atttacgtgt acgcagctga ccttcgatat
     8401 aaacctgaga acctttacgc agatattcgc tcgccacttc tgccagtttg ccgaacagca
     8461 caacgcggtg ccattcagtc tgttctttca tctcgccggt cgctttatca cgccaggatt
     8521 cggaagtagc cagcgtaatg ttggcaactg cgccaccatt tggcatgtag cgtacttccg
     8581 ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg
     8641 tgtctcctga aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc attaccaata
     8701 gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga cattgttaca
     8761 caacactccg ggtaatgcat tccaatactg tatattcatt caggtcaatt tgtgtcataa
     8821 ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt ttaatccggg
     8881 aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc aaaaacatca
     8941 acctcgttat cccccgcgac aagctcattg tcgtgaccgg gctttcgggt tctggcaaat
     9001 cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt gaatcccttt
     9061 ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat catattgagg
     9121 ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg cgttctacgg
     9181 tggggacaat cactgaaatc cacgactatt tgcgtttgtt gttcgcccgc gtcggcgaac
     9241 cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag atggtggata
     9301 acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct cgcgccaatc attaaagagc
     9361 gcaaaggcga acacaccaaa acgctggaga acctggcaag ccagggttac atccgtgctc
     9421 gtattgatgg cgaagtctgc gatctttccg atccgccgaa actggaactg caaaagaaac
     9481 ataccattga agtggtggtt gatcgcttca aggtgcgtga cgatcttacc caacgtcttg
     9541 cggagtcgtt tgaaaccgcg ctggagcttt ccggtggtac agcggtagtg gcggatatgg
     9601 acgacccgaa agcggaagag ctgctgttct ccgccaactt cgcctgccca atttgcggct
     9661 acagtatgcg tgaactggag ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc
     9721 caacctgtga cggccttggc gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc
     9781 ccgagctgtc actggctggc ggtgcgatcc gtggctggga tcgccgcaac ttctattact
     9841 tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg ccgtggggca
     9901 gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa aacattgaat
     9961 tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg tcatccgttc gaaggcgtgc
    10021 tgcacaatat ggagcgccgt tataaagaga cagaatccag tgcggtacgt gaagaattag
    10081 ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggaag
    10141 cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat ctccgacatg agcatcggtc
    10201 atgcgatgga attcttcaac aatctcaaac tcgctggtca acgggcgaag attgcggaaa
    10261 aaatccttaa agagatcggc gatcgtttga aattcctcgt taacgtcggc ctgaattacc
    10321 tgacgctttc ccgctcggca gaaacgcttt ccggcggtga agcccagcgt atccgtctgg
    10381 cgagccagat tggtgcgggc ctggttggcg ttatgtacgt gctggacgag ccatctatcg
    10441 gcctgcacca gcgcgataac gagcgcctgt tgggtacgct tatccatctg cgcgatctcg
    10501 gtaataccgt gattgtggtg gagcacgacg aagacgcaat tcgcgccgct gaccatgtga
    10561 tcgacattgg cccgggcgca ggtgttcacg gcggtgaagt ggtcgcagaa ggtccgctgg
    10621 aagcgattat ggcggtgccg gagtcgttga ccgggcagta catgagcggc aaacgcaaga
    10681 ttgaagtgcc gaagaaacgc gttccggcga atccggaaaa agtgctgaag ctgacaggcg
    10741 cacgcggtaa caacctgaag gacgtgacgc tgacgctgcc ggtgggtctg tttacctgca
    10801 tcaccggggt ttcaggttcc ggtaaatcga cgctgattaa cgacacactg ttcccgattg
    10861 cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc accgtatcgc gatattcagg
    10921 ggctggagca tttcgataaa gtgatcgata tcgaccaaag cccaattggt cgtactccac
    10981 gttctaaccc ggcgacctat accggcgtgt ttacacctgt gcgcgaactg tttgcgggcg
    11041 taccggaatc ccgtgcgcgt ggttatacgc caggacgttt cagctttaac gtccgtggcg
    11101 ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt ggagatgcac ttcctgccgg
    11161 atatctacgt gccgtgcgat cagtgcaaag gtaaacgcta taaccgtgaa acgctggaaa
    11221 ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa gaggcgcgtg
    11281 agttctttga tgcggtgcca gctctggcgc gtaagctgca aacgttgatg gacgttggtc
    11341 tgacgtacat tcgcctgggg cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg
    11401 tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat attctcgacg
    11461 agccgaccac cggtctgcac ttcgccgata ttcagcaact cctcgacgtg ctgcataaac
    11521 tgcgcgatca gggcaatacc attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg
    11581 ctgactggat tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct
    11641 ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc ttcctcaagc
    11701 cgatgctgta atcgttaagg ccgctttctg agcggccttt tcctttcaga gttgtaccag
    11761 caatttacgc ttttcttccg gtagtaaatt cactgcctgc tgataagacg catccaccag
    11821 ataatagatt tgcgaatccg gcagcgaacc gtcgagatac acggtgctcc agtgcgcttt
    11881 attcagatgg cggctaggac gcacatcgct gtgctgctga cgtagcagct ccgccagctc
    11941 cgggctggtt ttcagcgaaa cagctgggcg attttctact tctttcacca tcgcaaacag
    12001 tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc
    12061 tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact ccccttgtag
    12121 tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc agatgccttg
    12181 ccaggtgccg gtctgaatac gccctttatg taacggcaat acaagcgatg ttcccagcat
    12241 tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct cataatttcc
    12301 gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca
    12361 gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca gtaagccgat
    12421 gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca gatgaaaccc
    12481 acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct ccttgataaa
    12541 actctcttaa tcagtttgca gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag
    12601 cattcgggag agtgtcacgc tctcccgctc tgtcagtatt ctgaattgac gatcacctct
    12661 tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc gcgcagaatg
    12721 cggataccac gagcgccgac atcgcgtgca gcggtaatat cgttatcgga atcgccataa
    12781 aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc tggtttatcg
    12841 cccgcaaaga tcaccggatt catgttggtg acaggaatat gaaaattatc cgccagcgtt
    12901 tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca caaagaagat cgcgtcaccg
    12961 cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct gaattcatcc
    13021 cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc gctttctggt
    13081 gagaaggttt ttttgccgcg ccagaagccc gggctggaaa aaagtaccgt gtcatcgata
    13141 tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg tgcgaccgaa
    13201 acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt
    13261 gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa gcaaacggca
    13321 ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg ttgaaacttt
    13381 ttattgtgag atttgttgca aaaaccatct gaccataacg ccagctgccg gggaaaggaa
    13441 ggattatctg cggtttttgt gatgaaaagc aggatgatta tgtttaaccc agtaaatacg
    13501 ttcctgtctt tttttg
//
LOCUS       DAMZII010000128        13295 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZII010000128 DAMZII010000000
VERSION     DAMZII010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.128"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            148..1647
                     /gene="pitB"
                     /locus_tag="POA77_003750"
     CDS             148..1647
                     /gene="pitB"
                     /locus_tag="POA77_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDI6367116.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1696..2388)
                     /locus_tag="POA77_003751"
     CDS             complement(1696..2388)
                     /locus_tag="POA77_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI6367117.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2562..3275
                     /locus_tag="POA77_003752"
     CDS             2562..3275
                     /locus_tag="POA77_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI6367118.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLTALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3307..4065
                     /locus_tag="POA77_003753"
     CDS             3307..4065
                     /locus_tag="POA77_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367119.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4111..5460
                     /locus_tag="POA77_003754"
     CDS             4111..5460
                     /locus_tag="POA77_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI6367120.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5460..6284
                     /locus_tag="POA77_003755"
     CDS             5460..6284
                     /locus_tag="POA77_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367121.1"
                     /translation="MMRKYFPFEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLAPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6296..6856
                     /locus_tag="POA77_003756"
     CDS             6296..6856
                     /locus_tag="POA77_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI6367122.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6887..7957
                     /gene="lptF"
                     /locus_tag="POA77_003757"
     CDS             6887..7957
                     /gene="lptF"
                     /locus_tag="POA77_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDI6367123.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7954..9033
                     /gene="lptG"
                     /locus_tag="POA77_003758"
     CDS             7954..9033
                     /gene="lptG"
                     /locus_tag="POA77_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI6367124.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9069..10241)
                     /locus_tag="POA77_003759"
     CDS             complement(9069..10241)
                     /locus_tag="POA77_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6367125.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10241..10489)
                     /locus_tag="POA77_003760"
     CDS             complement(10241..10489)
                     /locus_tag="POA77_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6367126.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10521..11445)
                     /locus_tag="POA77_003761"
                     /pseudo
     CDS             complement(10521..11445)
                     /locus_tag="POA77_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            complement(11442..13172)
                     /locus_tag="POA77_003762"
     CDS             complement(11442..13172)
                     /locus_tag="POA77_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDI6367127.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         3211 a         3271 c         3441 g         3372 t
ORIGIN      
        1 ctaaaggttt tgaatgaatg cttattgtct gatacacaag aaataacact ctttttatcg
       61 ttaaaaaatg atatttcact ttgcccatgc cgttaaagtc actgataatg cgtccgttcg
      121 taaattcaaa atggcgtaat ctaatatatg ctaaatttat ttgttggcct tgatatatac
      181 acagggcttt tgttattgct tgctctggca tttgtgttgt tctacgaagc aatcaatggt
      241 tttcatgaca cggcgaatgc ggtggcaacc gttatttata ctcgtgccat gcaaccacaa
      301 cttgctgtgg tgatggcggc attttttaac ttttttggcg tgttattggg cggacttagc
      361 gttgcctatg ccattgtcca tatgttacca accgatttgt tgctgaatat ggggtcaacc
      421 cacggtctgg cgatggtctt ttccatgctg ctggcggcga ttatatggaa cctggggacg
      481 tggttcttcg gtttaccggc ctccagttcg cacaccttga ttggcgcgat tatcggcatc
      541 ggtttaacca acgcgctgtt aaccggctca tcggtgatgg atgcgttaaa cctgcgtgaa
      601 gtgaccaaaa ttttctcctc gctgattgtt tcccctatcg tcggcctggt cattgcgggt
      661 ggtctgattt tcctgctgcg ccgtttctgg agcgggacga aaaagcgtga ccgtattcac
      721 cgcattccgg aagatcgcaa aaagaaaaaa ggcaaacgta aaccgccatt ctggacgcgt
      781 attgcgctga ttgtttccgc tgcgggcgtg gcgttttcgc acggcgcgaa cgacggacaa
      841 aaagggatcg gcctggtaat gctggtactg gtggggattg cccctgctgg cttcgtcgtc
      901 aatatgaatg cgtccggcta tgaaattacc cgtacccgcg atgccgttac caacttcgaa
      961 cactacctgc aacagcatcc tgaactgccg cagaagttga ttgcgatgga acctccattg
     1021 cctgcagcat cgactgatgg cacgcaagta acagagtttc actgtcatcc ggcaaatacc
     1081 tttgatgcga ttgcgcgcgt taaaacgatg ctgccaggca atatggaaag ttacgagccg
     1141 ttaagcgtga gtcagcgcag ccagctgcgc cgcattatgc tgtgcatctc tgatacctcc
     1201 gcgaagctgg cgaaactgcc aggcgtcagt aaagaagacc agaacctgct gaaaaaactg
     1261 cgcagcgata tgttaagcac cattgagtac gctccggtgt ggatcatcat ggcggtagca
     1321 ctggcgctcg gcattggcac catgattggc tggcgtcgtg tagcgatgac catcggtgag
     1381 aagattggta agcgcggcat gacgtatgcg caaggcatgg cggcacaaat gacggcggca
     1441 gtgtctatcg gccttgccag ttatattggg atgcctgtct ccacaacaca cgtcctctcg
     1501 tctgcggtag cagggacgat ggtggtggac ggcggtgggt tacagcgtaa aacggtaacc
     1561 agcatcctga tggcgtgggt gtttacttta ccggcggcaa tttttctttc tggtgggctg
     1621 tactggatag cattgcagtt gatttaacct tcctgaaaat gcccggtcct ggcgatcggg
     1681 catttccatt tttgactagt gataaccacg cgcggtcata aaatccgtaa tcgctttttc
     1741 tgcatcaacc aacacctgtt ccagtggccg attggcatcg atatcaacca gttgtgcacc
     1801 accaaaggtt aactgtggcg ttatggcaat cttcctcgcc agcgattccc gtttatggtc
     1861 gggtttacgt gcacaggcaa cttcaaggtc aacattgagt ttgatgacca gatcaggctt
     1921 atggctcgcc atccagtgaa acgctttacg ttcctggctt gccagccatg agacaaaacg
     1981 accaccttca acgttaggtg ggaacaccgt accatcgtaa gcgccaggaa tttggtcctg
     2041 aggataacgg tcggttagaa caattaaccc gcgacgacga caggcaagca tatgacgaaa
     2101 gcgcagtaag cgacgggcga caaacgctgt aattaccagc gccggaactg gtcctggcaa
     2161 tttttttgct gttttcacct gatttcgttc aattgtttta tgtaaggatt ttcccatcaa
     2221 cggtaatttt gtcactgcac gaccgacatt tcccgcctgt ttccctaaat gaactctttc
     2281 ggcagcacca tatttttcga caacggtaat gagatgttca cacaccgttg acttgcctga
     2341 accatcacta ccaataacgg caattaatgg aggtgttatt gactgcatga atatatcctt
     2401 aagtttaaat atccatccaa aatatttatt tggttaatat gtttttatga aagcgtaatt
     2461 caggtcaatg tcacaattaa ccattgtcac aataaggttg aacggatatt ctgagcacgg
     2521 accacgtaat attcaataca ttatttgctg ccgggttttt cgtgaatcaa cgtaatatgt
     2581 caataattaa ttccacgcca gtgcgtgtta ttgccattgt aggatgtgat ggttcaggta
     2641 aatcgaccct cacggcaagc ctggtaaatg aactggcagc aagaatgcca acagaacaca
     2701 tttatctcgg gcaatcgtcc gggcgaattg gcgaatggat ttcacagctc cctgttattg
     2761 gcgcaccttt tgggcgttat ctgcgaagta aagcggcaca tgtgcacgaa aaaccctcaa
     2821 caccgcctgg caatattact gcgctggtta tctatctgct ttcctgctgg cgggcgtaca
     2881 agtttcgcaa aatgttgtgt aaaagccagc aaggctttct gctcatcacc gaccgctacc
     2941 cgcaggttga agtgccgggt tttcgctttg atggcccgca attggcaaaa accacgggcg
     3001 gtaacggttg gataaaaatg ttaaggcagc gcgagctgaa gctgtaccaa tggatggcat
     3061 cttatttgcc cgtattgttg attcgtcttg gcattgatga acaaacggcg tttgcgcgta
     3121 aacctgacca ccaactgaca gcgttacagg aaaaaatcgc cgttacgccg caactgacat
     3181 tcaatggtgc aaagatcctt gaactggatg gtcggcaacc agccgatgaa attctgcaag
     3241 cgtcactacg cgcaattcac gccgcgcttt cctgatcacc aaaatggata ggggatgatt
     3301 taacgaatgg atgcactaca aacacaaact gttattagta caaccgcgcc gcagcccagc
     3361 tacattccgg ggctaattgc ggtggtcggg tgtgatggca ccggtaaatc cacactgacc
     3421 accgacctgg tgaaatcgct gcaacaacac tggcaaaccg agcggcgcta tctggggctg
     3481 ctctccggcg aagacggcga caaaatcaaa cgattgccgt tggttggcgt ctggctggaa
     3541 cggcgactgg cggccaaatc ctcgaaaacc caaagcatga aaaccaaatc tccggcgcta
     3601 tgggcggcgg tgattatgta ctgcttctcg ctgcgaagaa tggcgaatct acgcaaggtt
     3661 cagcgactgg cgcaaagtgg cgttctggtg gtcagcgatc gcttcccgca ggctgaaatt
     3721 tcgggctttt attatgatgg accggggatt ggcgtcgaac gtgcgaccgg gaaaatcagc
     3781 atgtttctgg cgcagcgcga acggcgttta taccaacaaa tggcgcaata tcgcccggaa
     3841 ttaattattc gcctgggcat tgatattgag actgccatct cccgcaagcc tgaccatgac
     3901 tatgccgagc tgcaggacaa aatcggtgtc atgtcgaaga ttggctataa cggcacaaaa
     3961 attcttgaga ttgattccag agcgccctac agcgaagtgc tggaacaggc acagaaggcg
     4021 gtttctctgg ttgccatcgt ttctgaccgc cgaagtttaa cttaaggctg gcgggatact
     4081 ttttactatc aacgataagg tcaggctgaa ttggcgggtt ttaacatcaa acattggttt
     4141 gcagatggcg cgtttcgcac cattattcgc aatagcgcct ggttaggctc cagtaatgtc
     4201 gtgagcgcct tgctggggct gttggcgctc tcgtgtgccg gtaaagggat gacgcccgcc
     4261 atgtttggcg tactggtgat tgtgcaatcg tacgccaagt cgatcagcga ttttattaag
     4321 tttcagacat ggcagctggt ggttcagtac ggaacgccag ccttgaccaa caataatccg
     4381 cagcaatttc gcaatgtcgt ctcattttcc ttctcgctgg atatcgtcag cggcgcggtg
     4441 gcgattgtcg gcggcattgc cttactgcca ttcctttccc attcattagg tctggatgac
     4501 caaagctttt ggctggcggc gctgtattgc acgctcattc cttcaatggc ttcctccacg
     4561 ccgaccggca ttctgcgtgc ggtagatcgc ttcgatttaa ttgctgtaca gcaggcgacg
     4621 aaaccttttc tgcgcgcagc ggggagcgtc gtagcctggt attttgactt tggttttgcg
     4681 ggttttgtta ttgcctggta cgtgtcgaat ctggttggcg gcaccatgta ctggtggttt
     4741 gcagcgcgcg aattacgccg cagaaatatc cataatgcct tcaaattgaa tctgtttgag
     4801 tctgcccgac acatcaaagg cgcgtggagt tttgtctggt caaccaacat tgcccactcc
     4861 atctggtcgg cgcgtaactc gtgcagcaca gtgctggtgg ggatcgtgtt aggacccgct
     4921 gccgccgggt tatttaaaat cgccatgaca ttcttcgacg ccgccggaac gccagcgggt
     4981 ttgctgggta aaagttttta cccagaggtg atgcgtttag atccgcgcac caccagaccg
     5041 tggttgttag gtgtgaagtc cggtttactg gcgggcggaa tcggtattct ggtggcgctc
     5101 gcggtgttga tcgtcggcaa gccgctcatt tcgctggtgt ttggcgttaa gtatctcgaa
     5161 gcgtatgacc tgattcaggt gatgctgggc gcaattgtga tttcgatgct gggcttccct
     5221 caggagtcat tgctattgat ggccggaaaa cagcgcgctt ttctcgtggc gcaaaccatc
     5281 gcctcaatcg gctacatcgt attgctgttc atgttctgtc atctgtttgg tgtgctggga
     5341 gcagcgtttg cttacttcgg tgggcaatgt ctggacgtgg cgctctcgtt aattccaacc
     5401 ttaaaggcat tttttcagcg ccattccttg ctttataacg cagccggaga gaaatcctga
     5461 tgatgagaaa atatttcccg tttgaggcga gcgagcgtct atttgttgcg attgaagaag
     5521 atgatgtcgt tgatgcacag gttagtttgc cgccgactat tgcactaagt tgtaccaccg
     5581 aaatcattca tgataattac gcgttatgcc tgcaattctg gctcaatggc gttaatcgtc
     5641 aggagttact gcgtttagtt cgcaaacaag ccaaaggcga tgagctgact gctgatgagc
     5701 gcaaacaatt taaatacatg cgagcgcgct acaagcacct gcgttttgcg caacggctgt
     5761 atttaaagaa gcatcaggcc ggatttttat tcggcaaaac gaccgttttt ctggggcgtt
     5821 ttcaggacgg ctttcgtaac ggtaagaaaa atattgtgtc gtattacggc aacctgttac
     5881 ggatttattt aagctctccg gtctggtcgt tggtgaacta ttcattacgc catagccagt
     5941 tagaaagcgt aagcagtttt attgcctatc ggcaaaagca aatgcatacc ctgaaagaga
     6001 ttattgccaa accgcgacta accggcaggg aatttcatga tgtgcgaaaa atcatcagcc
     6061 aacaggtttc ttattacgac accttaagat cgcttgctcc tgaaaataaa gaggcgttac
     6121 agatttcccg tttcctcgcc gccattaatg gcctgatggg agataaacac gacgacatgg
     6181 tggcggatga tatggaaaac cgccagtcgt atgatgcacc gttggcgctg gatagcgata
     6241 ttcgtcagcg cttagagctg ctgatttcgc gttttccgct atagccggag caatgatgtc
     6301 atctcgtttg attattgcgt tgattatcat gctcttagcc ccgggtgtgc aggcacacaa
     6361 tttcgtcact ggcaaaacgg ttacccctgt ttatatccag gaaggcggcg agctgcttct
     6421 gaatagcgac gacgaaattc actaccaaaa gtggaacagc acacaattag caggaaaagt
     6481 ccgcattatt cagtatatcg ccgggcgtaa gtcggcgaag aaaaagaact cactgttaat
     6541 taaagcggtt gaagcggcaa actttccgca ggatcgtttt cagcccacca ccattgtgaa
     6601 caccgatgat gccatctttg gtactggcta ttttgttgtt ggcaaaatcg aaaaaaataa
     6661 acgccgctat ccgtgggcgc aattcattat tgacggcaat ggacagggac gcgttgcctg
     6721 gcgcttgccg gagcagagtt caaccattct ggtgctgaat aaagccgggc aaattcagtg
     6781 ggcaaaggac ggcagcctga cgccggaaga ggtagatcat gtcattgcgc tggcgcagaa
     6841 actgatcaat gaataaaaaa ttcaaaggat tgagggcgtt agtgagatga agctggttga
     6901 acactacatc atgcgtggta cgcgccgtct ggtgctgatt atcgtcggtt ttctgatctt
     6961 tattttcgcc agctactccg cacagcgtta tctgaccgaa gcggcaaatg ggacattagc
     7021 tcttgatgtt gtgctggata tcgttttcta caaagtgctg attgcactgg agatgttgtt
     7081 acctgttggt ctgtatgtgt cagttggcgt gacgctaggg cagatgtata ccgactcgga
     7141 aattaccgct atctctgcgg cggggggcag tccgggacgc ttgtacaaag ccgttcttta
     7201 tctggcgata ccgctaagta tttttgtcac ccttctgtcg atgtatggtc gtccgtgggc
     7261 ttatacgcag atttatcaac tggagcagca gtcgcagtcg gagctggatg ttcgccagtt
     7321 gcgggcaaag aaatttaaca ctaacgataa cggacgaatg atcctttcgc agacggttga
     7381 tcaggataat aatcgcctga ctgacgcgct gatttatact tctactgcca atcgaacccg
     7441 cattttccgc gcccgttcgg ttgatgtggt tgacccatca cccgagaaac cgaccgttat
     7501 gttgcacaac ggcactgcct atcttctcga tcatcagggg cgtgacgaca acgaacagat
     7561 ctaccgtaat ctgcaattac atctgaatcc gctggatcaa agccctaacg tcaaacgcaa
     7621 agcaaaatcg gtcacggagc tggcgcgctc cgcctttcct gccgatcatg ccgaactgca
     7681 atggcgacaa agccgtggcc tgaccgcatt gttgatggcg ctgctggcca tttcattaag
     7741 tcgggtaaaa ccgcggcaag ggcgattttc aacgttattg ccactgacgt tgctgtttat
     7801 tgccattttt tatggcggcg acgtctgccg tacgctggtg gctaacggcg cgattcctct
     7861 cattcctggt ttgtggttag tgcccggact catgctaatg ggcctgctga tgctggtcgc
     7921 acgcgacttc tctttgctgc agaaattttc ccgatgaatg ttttcagtcg ctatttaatc
     7981 cgtcatctct ttctcggttt tgccgccgcc gcagggctat tgctgccgct ttttaccacc
     8041 ttcaacctga ttaacgaact ggatgatgtc agcccgggcg gttatcgctg gactcaggcg
     8101 gtgctggtgg tgctaatgac cttaccgcgc acactggtcg aactttcgcc gtttatcgcc
     8161 ttattgggag ggattgttgg cctggggcag ttatcgaaaa acagtgagct taccgccatt
     8221 cgcagcacgg ggttttctat cttccgtatt gcactggtgg cgctggttgc cgggatattg
     8281 tggactgttt cgttaggcgc gatagatgag tgggtggcgt cgccattgca gcaacaggcg
     8341 ctgcaaatca aatcgaccgc caccgcgttg ggggaggacg atgacattac cggcaatatg
     8401 ctgtgggcca ggcgcggcaa tgaatttgtg acggtgaaat cgctgaatga acagggccag
     8461 cctgtgggcg tggagatttt tcattatcgt gacgatcttt cgctcgaatc ctacatttat
     8521 gcacgcagtg ccaccattaa agacgacaaa acgtggatcc tgcatggtgt gaatcataaa
     8581 aaatggctta acggtaaaga aacgctggaa acatcagata atcttgcctg gcaatcggcc
     8641 ttcaccagta tggatcttga tgagttatcg atgccgggga atactttttc tgtccgtcag
     8701 cttaatcatt acatccatta tttgcaggaa accgggcaac ccagcagcga ataccgcctt
     8761 gcactgtggg aaaaactggg gcaaccgatc ctgaccctgg cgatgatttt gctggctgtg
     8821 ccgtttacct ttagcgcccc gcgctcgcca gggatgggta gccgtctcgc tgtaggcgtc
     8881 atcgttggct tactcacctg gatcagctat caaatcatgg tcaatttggg attgctattt
     8941 gcgttaagcg cacctgttac cgcgctcggt ttaccggtag cgtttgtgtt ggtggcgttg
     9001 agcctggtgt attggtatga cagacaacat taaacccgcc gtggcgcaaa caccacggcg
     9061 ggctcacatt actgcttcag agtagcaaat gcctgaatga tggcatcgat ttgctcacga
     9121 ctgtgggcag catttacgct acagcgcagc agcgtgatgc ctgcaggcgc ggcaggcggc
     9181 aggataagat tcacatacac gcccagcgag ataagctcgc gccagatgcg taaaccttcc
     9241 tctttcgaac cgattattac tggcactacc gggctaatat gtgaacttaa ctcataaccc
     9301 agtttttgca gaccgtcata cagatggttg gcgttatcca tcagcttttg ccgcagctct
     9361 ggttgtgtgg caatggttct taacgatgag cgcacggtcg caatgcagga cggagaagga
     9421 gaggcggtaa aaatatacgg gcggctgccg tagcgcagca cttccatcgc ttccgaaccg
     9481 acagcaaaac cgccaatcga ggccagactt ttactgaaag taccaacgat aatatccaca
     9541 tcatcttcaa cgccgacagc ttcagccagt ccgcgccccg ttgcgcctaa cacgccaaac
     9601 gaatgcgctt catcgacaat caaataacca ccgagacgac gtttgatatc aacaatttcc
     9661 gccagcggcg caacgtcacc gagcatgctg tagatgccct caacgatgat aatcgcctct
     9721 ttcgcccgtt caccgaggcg aaccatgcgc cgttccagat ctttagcatc gttatggcgg
     9781 aaacgaataa tttccgcgcc accgagagaa caggcgtcat aaatactggc gtggctgtcg
     9841 gcatccagta acaccacggc gttatggtcg gcaagtgcgc tgatgacgcc taaattagcg
     9901 gtataaccgg ttgaaaaaac gatagcggta gggcgattga aaaactcggc aatctctttc
     9961 tctaacgcca ggtgcggcgc ataactgccg tttgccatac gagaaccggt ggtgccggta
    10021 ccttgggccg ctaacgcggc ctgaccttcg ctgatggcat catgattaaa ggttaaaccg
    10081 agataattat tggtcccggc cagaatcact tttttattgc cgatccggcc ttcggtggcg
    10141 gaatacactt catcaataca ggtgccaaac ggtgttaacc cgttgtcact aaattgcagt
    10201 cgctcgccag caaggcgcgc atatttatcg tatagcccca ttatttattc tccagccatg
    10261 gaagtagagt ttccattaat tgcgcagggg ttttgacatc cagcagaata ttaataggaa
    10321 tggaaatatc gaatctgtct tccagcatca ttaagagatc catcactttg atcgactcca
    10381 ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt tcgaccaaat
    10441 cctgtaaaca ggaaaggatg taatccatta ctatttcacg atttaccata gaaacgttaa
    10501 cacaccttta aacaagacaa tcaaaatagc ccgttgcgta atgcgtgttc caggctaatc
    10561 ccgggaaacc aattaatatc ttcagaaata cggttattac ttgccgacca gtcggcgtgg
    10621 gttaattcac gaattttcga gcgggtcagc ataggttctt tacctgccag gcgactcaac
    10681 gcggtactga tatccgcaag gcaggtgagt accggcagag gaataccaac cattcgcacg
    10741 gaaccgcaac ggacgttggc ggcaagttgc tgtatgcgtt gccagtcata gccgccagcg
    10801 acgccatcgc ataattcata ggtttgcgtc tgtacagttt cggcgcttaa ccactgaccc
    10861 actgcttgcg caaaatcggt gacgtgcagg aaagagagct gtgtatctgg tgcaccaagt
    10921 cgtggcagca agccgcgcag catccagtca aacagcggtt ttaactcttt atcgccggga
    10981 ccatacacgg ctgtcgggcg aaaaacgccc agcgtaattt cgtcagccat tgcagtcagc
    11041 cgttgttcgg cgatgtgttt ggaatttgcg taccaggaga gctcgggatg gcgcgccgcc
    11101 agcgaagaga taagaagaga taaacagaaa acgttggcaa aagcagctct cttttgctgc
    11161 ttgcatcagg cgcaggctgc cgtcaacgtt acagcgggtg aaaatctctt ctttgtgccc
    11221 gcgcacttgc ccggcgcaat ggaccactgc gctggctccg gcaaccagct cgctaagtga
    11281 atgagtatct tccagcgaac cgcgcaccca ggtgagatta tcattgacgt gagcgcgggc
    11341 agtacgcgtc aatgcgcgaa cgtgaaagcc gcgggcgagc aggttatcga taatatattt
    11401 accgataaac ccggtagcgc ccgtcaccgc gacagtttga ttcatgccag ggattcctgc
    11461 acatgaaggc ttgcagcata agccttctga taacgttttt tcgcttccgc acgggcaggc
    11521 ttgccggagg atgttcgggg aatactgtgg ggcggcaaca gatcgatagc cgctgttacg
    11581 ccaaattcgc tttggatccg tgctgccagc gcgtggataa gctgtccgcg acgttcttca
    11641 tcgctgatcc gacactggat ctgcaaaatg attttttcct gggcggtaac aaaagcaatc
    11701 gcatcgccag aatgaatttc cggttcctgt tccgctatat attcaatatc ctgcggccag
    11761 atattacggc cacgaataat aatcagatct ttaatgcgtc cggtgacata cagataaccg
    11821 tccagcagat aaccgaggtc gccggtgtct aaccagcccg tcgcggcaat ctcgtcttgc
    11881 gaagcctggt cgccaaagta accgctcatc agactggggc cggagatgca aatatggcct
    11941 accacacgtt ccgcgaccgg catacctgct tcattgcgga tttcaatacc atgttccggc
    12001 aacgctttgc cgcagttgac gaaagtcgat acggcgcgtg tctctgcacc cggcgcgacg
    12061 gctttaccct gatattcgag gatgtcgcga tccacttcgt taaccacaac cccagagcct
    12121 tcatcagaga agctgacagc cagcgcattt tctgccagtc cgtagcacgg catgaacgtt
    12181 ttatcgtcaa agttaacctg acggaaacat tcagcgaatt gatggagttg ttctgcggag
    12241 atcggctcag caccaatacc agcgacgcgc cagcaggaaa gatccagttc agcgagatct
    12301 ttttcattca cgcggcgttg gcacaattca tagccaaacg gcggcgcaac ggaaacggtg
    12361 ccgcgatttt tactgatcaa tttaagccat tgcaaaggac gcatggcaaa atcctgagtg
    12421 cgcaaataat ctactgaaag ttgcgtggcg acgggggtca ggagaaagcc gaccagtccc
    12481 atatcatggt agaaaggcag ccaggagacg cagcggtcgc cagggcgtaa tttaatgcca
    12541 tcgtggctta tagcacgtag attagccatc acttcgcgat gggtgataat gacgccacgg
    12601 ggaaaacggg tgctgccgga ggtgtactgg aggtaggcga tatcgttcgg aactggacgc
    12661 tggagcgcaa catcggcttc cggtaatgcc ttaaaccagg cgtggcttaa aacatgtaat
    12721 tcggggttgt tatgcgtcgc ggcattgacc agtggcaacc actcatcacc agtgataatg
    12781 gctgcgggct ggcaacttgc cagtaaaccc tggagtttgg cgctccagga atcccgctga
    12841 ccgacgccca ttggaatcgc caacgggacg gcgactaagc cagcatactg acaggcaaaa
    12901 aaagcctcta caaacccgct actcgtttcg gcaatcagcg ccacgcgttc gccttttttc
    12961 aggttcagcg ataacaaccg cttcgcacca gcttcggcac gcgtttttaa cgtctgatat
    13021 tccagttgat cttcaagttg gcaacgtctg tcataaaaat tcattccggc gctactcaga
    13081 gcggcgtagt ccaaagcatc aaccagcgtt ggaaaatcgg catagcgcat aggtagggaa
    13141 tgcgtaaaga ttttattaga catatacacc acgaaaaaat tggatatcaa aaataaaatg
    13201 acaataatgc ggtggattat ataggcataa aagataaatg agatagtatt ttcatcggtg
    13261 tgaaaatgaa cattgattta aataatgagt ttaag
//
LOCUS       DAMZII010000129        13160 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZII010000129 DAMZII010000000
VERSION     DAMZII010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.129"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(176..388)
                     /gene="cspH"
                     /locus_tag="POA77_003763"
     CDS             complement(176..388)
                     /gene="cspH"
                     /locus_tag="POA77_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDI6367128.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            674..886
                     /gene="cspG"
                     /locus_tag="POA77_003764"
     CDS             674..886
                     /gene="cspG"
                     /locus_tag="POA77_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDI6367129.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            897..1085
                     /locus_tag="POA77_003765"
     CDS             897..1085
                     /locus_tag="POA77_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6367130.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQICTDHAPLDFTK"
     gene            1060..1290
                     /gene="ymcE"
                     /locus_tag="POA77_003766"
     CDS             1060..1290
                     /gene="ymcE"
                     /locus_tag="POA77_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDI6367131.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1280..1453
                     /locus_tag="POA77_003767"
     CDS             1280..1453
                     /locus_tag="POA77_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDI6367132.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1501..2574)
                     /locus_tag="POA77_003768"
     CDS             complement(1501..2574)
                     /locus_tag="POA77_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI6367133.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2646..5390)
                     /gene="torS"
                     /locus_tag="POA77_003769"
     CDS             complement(2646..5390)
                     /gene="torS"
                     /locus_tag="POA77_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDI6367134.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPTLNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QRQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5473..6501
                     /gene="torT"
                     /locus_tag="POA77_003770"
     CDS             5473..6501
                     /gene="torT"
                     /locus_tag="POA77_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDI6367135.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6474..7166)
                     /gene="torR"
                     /locus_tag="POA77_003771"
     CDS             complement(6474..7166)
                     /gene="torR"
                     /locus_tag="POA77_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDI6367136.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7296..8468
                     /gene="torC"
                     /locus_tag="POA77_003772"
     CDS             7296..8468
                     /gene="torC"
                     /locus_tag="POA77_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDI6367137.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8468..11014
                     /gene="torA"
                     /locus_tag="POA77_003773"
     CDS             8468..11014
                     /gene="torA"
                     /locus_tag="POA77_003773"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDI6367138.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVIKWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            11011..11610
                     /gene="torD"
                     /locus_tag="POA77_003774"
     CDS             11011..11610
                     /gene="torD"
                     /locus_tag="POA77_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDI6367139.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11703..12008)
                     /gene="cbpM"
                     /locus_tag="POA77_003775"
     CDS             complement(11703..12008)
                     /gene="cbpM"
                     /locus_tag="POA77_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDI6367140.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12008..12928)
                     /gene="cbpA"
                     /locus_tag="POA77_003776"
     CDS             complement(12008..12928)
                     /gene="cbpA"
                     /locus_tag="POA77_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDI6367141.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3208 a         3335 c         3438 g         3179 t
ORIGIN      
        1 agtttcaagt ggtactataa ctggctgtta aatggcattt ataactatta ggtgcagaga
       61 tattcgctta aatggattag tttctttgtg gaaaagtaac tgatgttata atgtatgatg
      121 ttgactattg cactattcca ataaaaccag tcagctttaa acaagcagcg tcatattaag
      181 agagataaac atttgccgct gttggtcctc gcaggccatt tacgcggcaa aattccacac
      241 gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa tgcggaaata tggacctgaa
      301 cttctttacg accgtcggag gggataatga atcctttgcc gcttttgcga tcaaaggttt
      361 tgacaattcc tgtcatttta cgggacaaac aaattcctta ctgaaaatac tgcgctgcac
      421 tatacggggt taataaaata aagccagcga tatttaagac cgccggacgg ctaaaataaa
      481 atttgcttaa tctcaattat catgcgttaa tagctgcgtc ggtttgaaag acagacagca
      541 tacaaagtag tttactaaag cagttctcat tatcaggcat tatccccttc ttttgagtct
      601 ctctcctgaa cactaagtag tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg
      661 aaggaagtaa aatatgtcta ataaaatgac tggtttagta aaatggttta acgcagataa
      721 aggttttggc tttatcactc ctgatgatgg cagcaaagac gttttcgtcc atttcaccgc
      781 catccagagc aatgaattcc gcacgctgaa cgaaaatcag aaagttgaat tttctattga
      841 gcaggggcaa cgtggccccg cggcagcgaa cgttgttacg ctctaaggtt gccgttatta
      901 ctcaacatct ccatttccgc tgtccatgtt gtcatggttc acagtaccgt acatcggcat
      961 tcgatgtgac ggagcgaaac cctttgggcg ctaagtgtat tttttgtaaa tcgacgatga
     1021 tcacctttga taacgtcgcg ctgcaaatat gcacggacca tgcgccgctg gatttcacaa
     1081 aataatatca ggctccctcg tggagccttt tttatatctg ccttattttt cttcaacgct
     1141 gtatgtatag taagcgataa cctgttgatt attgaatctt tcggggagat ggcttataac
     1201 atttcttacc tgaccagggt accgggaacc aacaccttac tggcgtgttg ctgtctttca
     1261 agaccagaag aggttaacag tgaatattga agagttaaaa aaacaagccg aaacggaaat
     1321 cgccgacttt atcgcgcaaa aaatcgccga gctgaacaag aatacaggga aagaagtctc
     1381 tgaaattcgc ttcaccgcac gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat
     1441 caaaataatg tgattttgtg aacatcaccc gtgcgaggtg atgttccgct tgttgctaat
     1501 ttagtgacca atcattggcg cttgtggaat taagcgtcgg tacaattcat ccggtaccgg
     1561 gctttgccat actcccgcat acattgcgta accaatcacc gcaaacataa tccccagaac
     1621 cagtagcgtc attaaccagc cagacaacgc aaaggctttt ttatttgccg caggtttttg
     1681 cagtgaaaag gtcaatgttg aggctaccgg acatgactct acgcaagtca tacagccggt
     1741 acattccact gttcgtacct gaattaattt atcgaccggg atccgtgatg ggcaattttt
     1801 tgcgcatttg ccacagtcga tacaactttc ggcattgcga cgaatcttaa acggcgacaa
     1861 tagcgaaacc acgcccatca gcgcgccata tgggcaaaga taacgacacc aggcatggcg
     1921 aataaacagg ctggcaatca gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg
     1981 acgaaagaaa tcgagcattt taacgtccat caccacgctg tagggcgaca acataaaata
     2041 gtgaatcgcc tgagcgggca tcaataacgc gatatagaga aaaaaactca acagcaaata
     2101 cttcacgccg cgcagaggaa tatccagcca gcggggaagg acacattgcc gaccaaacag
     2161 tttgttaccg agatcgccga ttaattcaga aagcgtacca accgggcata accatgagca
     2221 aaaggccttt ttgagtaata gactgatgac gataaaagcg accaataaca gcatcgcggc
     2281 ggcgtggacg gacggtaact gacctgttac aaggctatat ttcagattca tcagcccggc
     2341 aatcggtagc cagccttcga tacctcccgg tctggcgaca aatgtcgtgc tacttgccgt
     2401 ttcgtaatag cgcacccaat accagaacgt gatggcaata taaatattca ttgccaacag
     2461 taataattgc gtcgctttac gccaggtcgt ggcattacgc cagtcattcc acggtaattt
     2521 gccgcccgtc gtgcctggcc gccgctgcca gcgggttctt ttattctctg ccatgatttt
     2581 gccagtccgt taagttgtat accaaatgcc actattctag ttgttcttaa ctggctgata
     2641 ttgattcaaa tcgcgttcag gtctttctta tgcaaccatg cttccagagc ggcaacactg
     2701 cgtgtaattt cttcgtgtgg aaggggggca gataatggct gctgctccag ttgtgcgcat
     2761 agctggctgg cgatacgcat tcccagactc gagcaactgc tttttagctg atgcgcggca
     2821 cgctttattt tctcgctatc ctgactggcg cgggcaatgt cgatttcatc gagaagcggc
     2881 agggcatgtt gtgtaaataa taccagccat tcgtggatct tctccgtccc cattaactga
     2941 gcatcttcat tgagttgcga tacatccagc gattgatcgt tattgacttg ccgttggaga
     3001 tagtgcgcca gtaactgacc gagcacttca cgcggcaccg gtttagggat aatcccgcgg
     3061 aataatgaac tggtacgctg gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc
     3121 aaaaccagcg acggatattg ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgacatcc
     3181 ggcagatcaa aatccaccag tgcggcagca aacggttcgc tattttgcag tgtctctaaa
     3241 gcctgcgcgg cattgccaac agcaacaacc tgcgcaccac tggtgttcag catctcaacg
     3301 gtaattcgct gggttagcgg gttatcttca attaacagca aacgtaaacc gtcaagacgc
     3361 accgcctgat tgactgtttt tggcacgggt gccgtggcaa cacgtaacgg caagcgtaaa
     3421 caaaaacagc ttccaacctc cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg
     3481 gccagacggc tactgatagt cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc
     3541 acaaatggct ggaagatttc tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc
     3601 acttcgacca gccattgctc gccatcagtg cgactacgca ggataatgta cccttcgtca
     3661 gtaaaacgca gggcgttgct caacaggttg gttataacct gacgaatacg tcgtggatcg
     3721 cccattaacg cgcacggcat atcatcggca attgccgttg ccaggcgaat cgggcgacct
     3781 ttcacccgtc cgctcattaa ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc
     3841 tcatcgctga ccgaaacatt cttgccacct gcttcgatag cggaataatc gagaatatcg
     3901 ttgaggatgg tcagcaacga ttcaccagag tcagtaattg cccgcaaatc atcacgctgg
     3961 gcgttaagtg tggggttatc tgccagtagc tgtgcggtgc cgagaatacc gtacagcggt
     4021 gtgcggatct catggctcat cgccgccaga aacgccgatt ttgcctggct ggctttttct
     4081 gcttccgccc gtgcctgtcg gtgttctatc accagttcct gcaattcagc tgtacgcgct
     4141 ttgacctgcg ccgccagctg ttcgcggtgg cgattcagtg catgaacact gctgcgaaac
     4201 gcatccatca gccgcccgat ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg
     4261 gagtcaatat caccgtccag cagccgttgc agcgcctgcg tttgttcggc aagtggacgc
     4321 gtgactgagc gataaaccac gcgccaaagg atcagaatca gtgcgcaaag tgaaaccatc
     4381 cccagcaata acaggctgta ttgcccgcgt gcactggctt tttccagatg cgccagtccg
     4441 tgctgattac gcagctcaat ggtgtcgacc agctgactga cttcgctact aaactgcgcg
     4501 aactgggcga tgttattttg tgcgagagtt tgtaggtgat tgctgatttc actgtcctgc
     4561 tgatacagcg ccagcaaatc gctatattgg ctaacggtag ttaacgttgt tgcgacctgc
     4621 gcacgaacac ccggatcttc aatgcgtatt tgccgacgtt gcagaatttt caccgcatta
     4681 ttgagctgct tttccagcgt tggtgcattt ttctggatct gctccagccc cagattcatc
     4741 accatttgct gcacccgcag agcgctaagg cgcagttcat tcatctggtt aacatactca
     4801 agatcgatat caatcagccg atcgagtgca ctttcagcag cctgacgctg atcttgttcg
     4861 atcaaatcgt aaatcccggc ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga
     4921 ccttgcgcca ggcgtgcgat ctcatcggcg gcagcgacta tctgctgact gagttgccgt
     4981 tgttgctggc gtagttgcag acgccgcccc accagttccc cttgctgacg taatgaacgg
     5041 gagatctcct gctcctgttg ttcaatagcg gtggtatcaa aaccttgttc ccgtaacgct
     5101 tgcagcaacg cattaatctt caggctttgt gcggtgagca ttcgcccctg cgcctgccac
     5161 atcttttcgt tatcggcact ggtcaggttc tgcgcggcga aaagttccca ggcgctggct
     5221 tcgctcaact ggcgcgccat attcatggta ggaatcaatg cctgagtgtt gtctttttcc
     5281 acctggctga taaagcgcag gttgtaccat cccaccaggg tactggtcag ggttaacagc
     5341 gccatcaggg caaagcccat ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca
     5401 ctttaggtga aaaaggttga gtcgcaaagc agaatgcatc tagcataaag ccttattatt
     5461 gatgaggcta tcatgcgcgt actgctattt ttacttcttt cccttttcat gttatcggca
     5521 ttttcggctg ataacctgtt gcgctggcat gatgcgcagc attacacggt gcaagcctct
     5581 acgccgctta aagccaaacg cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat
     5641 tcatactggt tatcgttgaa ctatggtatg caggaggcag ctcgccgcta tggtgtggat
     5701 ttaaaagtgc tggaggcagg cggctacagc cagttggcta cccagcaagc acaaatcgac
     5761 cagtgtaaac agtggggcgc agaggccatt ttgctcggta gtagcactac gtcatttccc
     5821 gacctgcaaa agcaggtagc aaatctgccg gtgatcgaac tggtaaatgc tcttgatgat
     5881 cccctggtga aaagccgcgt tggtgtaccc tggtttcaga tgggctatca acctgggcga
     5941 tatttggtgc aatgggcgca cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat
     6001 aatgccgggg gcagtaaaga gatggtcgag ggttttcgcg cagccattgc cggaagcccg
     6061 gtgcgtattg ttgatattgc gcttggtgat aacgatattg aaatccagcg taacctgttg
     6121 caggagatgc tggaacgcca cccagaaatc gacgtcgttg ccggaacggc cattgcggca
     6181 gaggcggcaa tgggtgaagg gcgtaacctg aaaacgccgc ttaccgtggt gtcgttttat
     6241 ctttcacatc aggtgtatcg cgggctgaag cggggaagag tgattatggc tgccagcgat
     6301 caaatggtct ggcaggggga actggcggtt gagcaggcca tcaggcaatt acaggggcaa
     6361 tcggtgtctg ataatgtcag cccaccgatt ttagttctga cgccgaaaaa tgccgaccgt
     6421 gaacatattc gccgctcgct gtcaccaggg ggatttcgtc cggtctattt ttatcagcac
     6481 acatcagcgg ctaagaaata accttcacca tgttgcgtca ccagtaaatc cgcgctgagt
     6541 ttatgacgta aacgacgaat taacacatcg acggtgcgca ggtcagggtt ttccacccga
     6601 cgcgcagaaa gcatacgtag cagacgttca cggctgagaa tttcgcccgg attcgtcaca
     6661 aatgccacca acatttcata ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc
     6721 agcgtatggc gcgacacatt caggcaataa ccggcaaagc gatagcagtt gtcctgagtg
     6781 tgcggttgag cttgtcgcgc gaggtcgatt cgccagagca gatttttcac ccgtactacc
     6841 agttcgcgca gttccagcgg tttggtgacg taatcgtctg cgcccatttc cagcccaaca
     6901 atacggtcaa tccgatcgct gcgtccggta accagaataa tccccaccgt tgagcgttct
     6961 cgcagggcgc gggttaacat caggccattt tcatcgggta agttgatatc cagcagaatt
     7021 aaatctaccg gctgattctg cataatttcc cgcagcccgg caccgctcgc tgtaacggaa
     7081 acggtatacc cctcctgagt gaagtaagat tgtaatcgcg cctgggtaac cggctcatct
     7141 tcaacaataa caatgtgatg tggcatcaga gggttttact cattctgttc atatctgttc
     7201 atattctgcc gtaagccgtt catcctgacc agtgccgctg ttcatatttg ctcattaaga
     7261 tcgcttcact aaaccataat tttacagggg ctattatgcg gaaactctgg aacgcgctac
     7321 gccgacccag tgctcgttgg tcggtactgg cgctggtcgc aattgggatt gtgattggca
     7381 ttgcgctgat tgtattgcca cacgttggga tcaaagtcac cagcacaacc gaattttgtg
     7441 tcagttgcca cagtatgcaa ccggtgtatg aagaatataa acagtcggtg catttccaga
     7501 acgcctccgg cgtgcgagct gaatgccatg actgtcatat cccgccggat attccaggca
     7561 tggtgaagcg caaactggaa gcgagcaatg atatctacca gacctttatt gctcactcca
     7621 ttgatacacc tgaaaaattc gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc
     7681 gaatgaaaga aaacaactcg gcaacctgcc gctcctgcca taactacgat gcgatggatc
     7741 atgcgaagca gcatcctgaa gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat
     7801 cctgcatcga ctgtcataaa ggtattgccc accagttacc ggatatgagt agcggcttcc
     7861 gtaagcagtt cgatgagctg cgcgccagtg ctaatgacag tggtgacacg ctgtactcta
     7921 ttgatattaa gccaatttat gcggcgaaag gcgataaaga agcctctggt tctctgctgc
     7981 ctgcttcgga agtgaaagtc cttaaacgtg acggcgactg gctgcaaatt gaaattaccg
     8041 gctggacgga aagcgccgga cgtcagcgtg tactcaccca attcccaggt aaacgcatct
     8101 ttgttgcctc gattcgtggt gatgtgcagc agcaggtaaa aacgctggag aaaaccaccg
     8161 ttgccgacac caataccgag tggagcaagt tgcaggccac cgcatggatg aagaaaggcg
     8221 acatggtgaa cgatatcaaa ccgatctggg cctatgcgga ttcgttgtac aacggcacct
     8281 gtaaccagtg ccacggcgca ccggaaatcg cccactttga cgctaacggt tggatcggca
     8341 cgctcaacgg catgattggc tttaccagtc tcgataaacg tgaagaacgc actttgttga
     8401 aatatctgca aatgaatgcg tctgacaccg caggtaaggc tcacggcgat aagaaggaag
     8461 aaaaataatg aacaataacg atctctttca ggcatcacgt cggcgttttc tggcacaact
     8521 cggcggctta accgtcgccg ggatgctggg gccgtcattg ttaacgccgc gccgtgcgac
     8581 tgcggcgcaa gcggcgactg acgctgtcat ctcgaaagag ggcattctta ccgggtcgca
     8641 ctggggggct atccgcgcga cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga
     8701 actggataaa tatccgtcga aaatgattgc cggattgccg gatcacgtac acaacgcggc
     8761 gcgtattcgt tatccgatgg tacgcgtgga ctggctgcgt aagcgccatc tcagcgatac
     8821 ctcccagcgc ggtgataacc gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt
     8881 ctatgaagaa ctggaacgcg tgcagaaaac tcacgggccg agtgccttgc tgaccgccag
     8941 tggttggcaa tcgacgggga tgttccataa cgcttcgggg atgctggcga aagctattgc
     9001 cttgcatggt aatagcgttg gtacgggcgg agattactct accggtgctg cgcaggtgat
     9061 cctgccgcgc gtggttggct caatggaagt gtatgaacag caaacctcct ggccgctggt
     9121 attgcagaac agcaaaacca ttgtgctgtg gggctctgat ttgctgaaaa accagcaagc
     9181 gaactggtgg tgcccggatc acgatgttta tgaatattac gcgcagctaa aagcgaaagt
     9241 cgccgccggt gaaattgagg tcatcagcat cgatccggtt gtcacatcca cccatgagta
     9301 tctggggcgc gagcatgtga agcacattgc ggttaacccg caaactgatg tgccgctgca
     9361 actggcgctg gcgtatacgc tgtacagtga aaacctgtac gacaaaaact tcctcgctaa
     9421 ctactgtgtg ggttttgagc agttcctgcc gtacctgctg ggtgagaaag acggtcagcc
     9481 gaaagatgcc gcatgggctg aaaaactgac cggcattgat gccgaaacca ttcgtgggct
     9541 ggcgcggcag atggcggcga acagaacgca aattattgct ggctggtgcg tgcagcgtat
     9601 gcagcacggt gaacagtggg cgtggatgat tgtggttctg gcggcgatgc tggggcaaat
     9661 tggcctgcca ggtggtggct ttggttttgg ctggcactat aacggcgcag gcacgccggg
     9721 gcgtaaaggc gttattctga gtggtttctc cggctctacg tcgattccgc ctgttcacga
     9781 caacagtgat tacaaaggct acagcagcac cattccgatt gcccgtttta tcgatgcgat
     9841 cctcgaaccg gggaaagtga tcaaatggaa cggtaaatcg gtaaaactgc cgccgctgaa
     9901 aatgtgtatt tttgccggaa ctaacccatt ccatcgccat cagcagatca accgcattat
     9961 tgaaggctgg cgcaagctgg aaacggttat cgccatagat aaccagtgga cctcaacctg
    10021 ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt gagcgtaacg atctcgacca
    10081 gtacggcaac cactccaacc gtggcattat cgccatgaaa caggtggtgc cgccgcagtt
    10141 cgaggcgcgc aacgactttg atattttccg cgagctgtgc cgtcgcttta atcgcgaaga
    10201 agcctttacc gaagggctgg acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt
    10261 acaacaaggc aaaggacgcg gcgttcatct gccagcgttt gatgacttct ggaataacaa
    10321 agagtacgtc gagtttgacc atccgcagat gtttgttcgc caccaggcat tccgcgaaga
    10381 tccggatctc gaaccgctgg gcacgccgag tggcctgatt gagatctact cgaaaaccat
    10441 cgccgatatg aactacgacg attgtcaggg gcatccgatg tggtttgaga aaatcgaacg
    10501 ctcccacggt gggcccggct cgcagaagta tccgttgcat ctgcaatctg tgcatccgga
    10561 tttccgactt cactcgcagt tatgtgagtc ggaaactctg cgtcagcaat atacggtagc
    10621 gggtaaagag ccagtgttca ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg
    10681 tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg gcaggggcag tagtttctga
    10741 ccgctatgca cccggcgtgg cacgaattca cgaaggggca tggtacgatc cagataaagg
    10801 cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac gtgctgacca tcgacatcgg
    10861 tacttcgcag ctggcgcagg cgaccagtgc gcacactacg ctggtggaaa ttgagaagta
    10921 caatggagca gtggagcagg tgacggcgtt taacggccct gtggagatgg tggcgcagtg
    10981 cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc tgacagcaca acagattgcc
    11041 tgtgtttacg cctggctggc gcagttgttc tcccgtgaac tggacgatga acaactgacg
    11101 caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc tgaaaagcga accgccgctg
    11161 gctgcggcgg tgaatgagct ggaaaactgt attgccacgc tgacagtacg tgacgatgcc
    11221 cgtctggaac tggccgctga cttttgcggc ctgtttctga tgaccgacaa acaagcggcg
    11281 ctgccgtatg catcggccta caaacaggac gagcaagaga ttaaacgctt gttagttgag
    11341 gcagggatgg aaaccagcgg caatttcaac gaaccggcag atcatctggc gatctatctc
    11401 gaattgctca gccatctgca tttttcgctg ggagagggga ccgttcctgc gcgaagaatc
    11461 gacagtttgc ggcaaaaaac actgacggcg ctgtggcaat ggttaccaga gtttgttgtg
    11521 cgttgtcgtc agtatgacag ctttggtttt tacgcggcac taagccagtt attgctggtg
    11581 ttagtggaga gcgaccacca aaacagataa cgtcgtttgt gcgcctgaaa agacgcgttt
    11641 agcgtcgtat caggcattat ggggcagttg ccggatgcgt catcacatcc ggcaaacaca
    11701 catcacggat gagctacaaa ccgggaaagt cgctggcgca gcaggcggtt ttcctgcttc
    11761 aggtgcgcaa tatcatccat taacgtcagc gccaccgcga tcccaggcca gtccagagcc
    11821 agttcatgac gcaggcgtac tgcgcgttgc accacgatgg cggcatggtc gtcaaatacc
    11881 caggttgttt cctggatctc acgcggttca accaccccca aaccgacaat ttcattcaac
    11941 tcctcttcag agatgccggt atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca
    12001 ttagccatta tgctttcccc caatctttac gtggatcaaa agacgactgg gcgtctgcca
    12061 gttgctgcca cagcgcggca gtgttttcat ccggtttcgg cggcatcacg attttcagta
    12121 ccgcatacag atcgccggtc tgttttttgc tcaccagacc tttgccttta acgcgcaatc
    12181 gttgcccggc ctggctgcct ggcgggatag tcagcaaaat gctttctttc agtgttggaa
    12241 cggtgacttt agcacccagc gccgcttccc acgggctaac cggcaccaca atttccagat
    12301 cctggccgac aatatcgaac agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc
    12361 catttggacc accgttttcg cccggcgtcc cctggccttt cagacggatg cgttgaccat
    12421 tgccgacgcc cgccgggatc ttcacattca gcgttttcgg gatttcctgt tcgatcatgc
    12481 caaaggcgtt ataaaccggc aggttatagc tgatggtacg cttatgctca gtaagcgttt
    12541 cttcgaggaa taccgccact tcgatttcaa tatcgtggcc gcgtgcggcg gggcgttgac
    12601 ggctctggcg ggcatgctga ccgaaaattg acgagaagat atcgtcaaaa tcttcggcat
    12661 taaaactctg accgtcgcca tgatggaact gacggttaaa ttgcggatcg ttgcgatgtt
    12721 gccacatctg atcatactca gcgcgacgtt gttcatcact taacacttcc caggcttcag
    12781 cgacctcttt gaagcgggct tcggcatccg gttctttgct gacatcagga tggtatttac
    12841 gggccagtcg acgataggcg gtcttgattg tcttgagatc gtccgtcggt ttcacgccca
    12901 tgatggcgta ataatccttt aattccatag cgttatctcg cgtaaatcaa cacaaattga
    12961 aggaacccct gtaaggtaac tcctataagt gtagggtaat cctcaaaatt tcatatgcca
    13021 acacagaata tgttattgaa atcatcgcgg agaggaggtc gccatcaaga tgggttgctg
    13081 aacatatttt aaacaggtga aaaagggtga gcgatttttg atagttgaac caggcacttt
    13141 aagtttaact agggcgtcat
//
LOCUS       DAMZII010000130        13138 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZII010000130 DAMZII010000000
VERSION     DAMZII010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.130"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            223..1332
                     /gene="livK"
                     /locus_tag="POA77_003777"
     CDS             223..1332
                     /gene="livK"
                     /locus_tag="POA77_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDI6367142.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGAEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1380..2306
                     /gene="livH"
                     /locus_tag="POA77_003778"
     CDS             1380..2306
                     /gene="livH"
                     /locus_tag="POA77_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDI6367143.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2303..3580
                     /gene="livM"
                     /locus_tag="POA77_003779"
     CDS             2303..3580
                     /gene="livM"
                     /locus_tag="POA77_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDI6367144.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQV"
     gene            3577..4344
                     /gene="livG"
                     /locus_tag="POA77_003780"
     CDS             3577..4344
                     /gene="livG"
                     /locus_tag="POA77_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDI6367145.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4346..5059
                     /gene="livF"
                     /locus_tag="POA77_003781"
     CDS             4346..5059
                     /gene="livF"
                     /locus_tag="POA77_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDI6367146.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5455..6771
                     /gene="ugpB"
                     /locus_tag="POA77_003782"
     CDS             5455..6771
                     /gene="ugpB"
                     /locus_tag="POA77_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDI6367147.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6869..7756
                     /gene="ugpA"
                     /locus_tag="POA77_003783"
     CDS             6869..7756
                     /gene="ugpA"
                     /locus_tag="POA77_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDI6367148.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7753..8598
                     /gene="ugpE"
                     /locus_tag="POA77_003784"
     CDS             7753..8598
                     /gene="ugpE"
                     /locus_tag="POA77_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDI6367149.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8600..9670
                     /gene="ugpC"
                     /locus_tag="POA77_003785"
     CDS             8600..9670
                     /gene="ugpC"
                     /locus_tag="POA77_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDI6367150.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9667..10410
                     /gene="ugpQ"
                     /locus_tag="POA77_003786"
     CDS             9667..10410
                     /gene="ugpQ"
                     /locus_tag="POA77_003786"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6367151.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10397..10837)
                     /locus_tag="POA77_003787"
     CDS             complement(10397..10837)
                     /locus_tag="POA77_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDI6367152.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10957..12699
                     /gene="ggt"
                     /locus_tag="POA77_003788"
     CDS             10957..12699
                     /gene="ggt"
                     /locus_tag="POA77_003788"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI6367153.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQAGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSQDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12737..13021)
                     /locus_tag="POA77_003789"
     CDS             complement(12737..13021)
                     /locus_tag="POA77_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDI6367154.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2920 a         3362 c         3761 g         3095 t
ORIGIN      
        1 tgctgacaaa ggcacttttt tctgtttatc tatcaataaa ttcagaatat tatctgttct
       61 taatcgactg aaaaataggg attttaatca ctattatcac aaaatactgc gctaaaccct
      121 taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt gttaacacat
      181 aaaaacaaag caacacaaca tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa
      241 actatcatcg cagggatgat tgcactggca atttcacaca ccgctatggc tgacgatatt
      301 aaagtcgccg ttgtcggcgc gatgtccggc ccgattgccc agtggggcga tatggaattt
      361 aacggcgcgc gtcaggcgat taaagacatt aatgccaaag ggggaattaa gggcgacaag
      421 ctggttggcg cggaatatga cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac
      481 aaaatcgtta atgacggcat taaatacgtt atcggtcatc tgtgttcttc ttctacccag
      541 cctgcgtcag atatctatga agacgaaggt attctgatga tctcgccggg agcgaccaac
      601 ccggagctga cccaacgcgg ttatcaacac attatgcgta ctgccgggct ggactcttcc
      661 caggggccaa cggcggcaaa atacattctt gagacggtga agccccagcg catcgccatc
      721 attcacgaca aacaacaata tggcgaaggg ctggcacgtt cggtgcagga cgggctgaaa
      781 gctgctaacg ccaatgtcgt tttcttcgac ggcattaccg ctggcgagaa agatttctcc
      841 gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg tttactacgg cggttactac
      901 ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac ccagtttatg
      961 gggccggaag gtgtgggtaa tgcgtcgctg tcgaatattg ctggcgatgc tgccgaaggc
     1021 atgttggtca ccatgccaaa acgctatgac caggatccgg caaaccaggg catcgttgat
     1081 gcgctgaaag cagacaagaa agatccgtcc gggccttatg tctggatcac ctacgcggcg
     1141 gtgcaatctc tggcgactgc ccttgagcgt actggcagcg atgagccgct ggcgctggtg
     1201 aaagatttaa aagctaacgg tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa
     1261 ggcgatctta agggatttga ttttggtgtc ttccagtggc acgccgatgg ttcatccacg
     1321 gcagccaagt gatcatccca ccgcccgtga aatgcgggcg ggtttagaaa ggttacctta
     1381 tgtctgagca gtttttatat ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta
     1441 cctacgcgct gattgccatc ggctacacca tggtttacgg cattatcggc atgatcaact
     1501 tcgcccacgg cgaggtttac atgattggta gctatgtctc ctttatgatc atcgccgcac
     1561 tgatgatgat gggcattgat accggctggc tgctggtggc ggcgggattc gtcggcgcaa
     1621 tcgtcattgc cagcgcctac ggctggagta tcgaacgggt ggcctatcgc ccggtgcgta
     1681 actctaagcg cctgattgcg ctcatctcgg caatcggtat gtccatcttc ctgcaaaact
     1741 acgtcagcct gaccgaaggt tcgcgtgacg tggcgctgcc gagcctgttt aacggtcagt
     1801 gggtggtggg ccatagcgaa aacttctctg cctctattac taccatgcag gcggtgatct
     1861 ggattgtcac cttcctcgcc atgctggcgc tgacgatttt cattcgctat tcccgtatgg
     1921 ggcgcgcgtg tcgtgcctgc gcggaagatc tgaaaatggc gagtctgctt ggcattaaca
     1981 ccgaccgggt gattgcgctg acctttgtga ttggcgcggc gatggcggcg gtggcgggtg
     2041 tgctgctcgg tcagttctac ggcgtaatta acccctacat cggctttatg gccgggatga
     2101 aagcctttac cgctgcggtg ctcggtggga ttggcagcat tccaggggcg atgattggtg
     2161 ggctgatcct ggggattgcg gaggcgctct cttctgccta tctgagtacg gaatataagg
     2221 atgtggtttc atttgccctg ctgattctgg tgctgctggt gatgccgacc ggtattctgg
     2281 gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc tgctctctgc
     2341 cgcgatgttt tttgtcctgg cgggcgtctt tatgggcgtg caactggagc tggatggcac
     2401 caaactggtg gtcgacacgg cttcagacat ccgttggcag tgggtgttta tcggcacggc
     2461 ggtggtcttt ttcttccagc ttttgcgacc ggctttccag aaagggctaa aaagcgtttc
     2521 cggaccgaag tttattctgc ccgcgattga tggctccacg gtgaagcaga aactattcct
     2581 cgtggcgctg ctggtgcttg cggtggcgtg gccgtttatg gtgtcgcgtg ggacggtgga
     2641 tatcgccacc ctgaccatga tctacattat cctcggcctc gggctgaacg tggttgttgg
     2701 tctttctggt ctgctggtgc tggggtacgg cggcttttac gccatcggcg cttacacttt
     2761 tgcgctgctc aatcactatt acggcctggg cttctggacc tgtctgccga ttgccgggtt
     2821 aatggcagcg gcggccggct tcctgctcgg cttcccggtg cttcgcttgc gcggcgacta
     2881 tctggcgatc gttaccctcg gtttcggcga aattgtgcgc atattgctgc tcaataacac
     2941 cgaaattacc ggcggcccga acggaatcag tcagatcccg aaaccgactt tcttcggcct
     3001 tgagttcagc cgtaccgctc gtgaaggcgg ctgggacacg ttcagtaatt tctttggcct
     3061 gaaatacgat ccctccgatc gcgtcatctt cctgtatctg gtggcgttgc tgctggtggt
     3121 gctaagcctg tttgtcatta accgcctgct gcggatgccg ctggggcgtg cgtgggaagc
     3181 attgcgtgaa gatgaaattg cctgccgttc gctgggctta agcccgcgtc gtatcaagct
     3241 gaccgccttt accatcagcg ccgcgtttgc cggttttgcc ggaacgctgt ttgctgcgcg
     3301 ccagggcttt gtcagcccgg aatccttcac ctttgctgaa tcggcgtttg tactggcgat
     3361 agtggtgctc ggcggtatgg gatcgcagtt tgcggtgatt ctggcggcaa ttttgctggt
     3421 ggtatcgcgc gaattgatgc gtgatttcaa cgaatacagc atgttaatgc tcggtggttt
     3481 gatggtgctg atgatgatct ggcgtccgca gggcttgctg cccatgacgc gcccgcaact
     3541 gaagctgaaa aacggcgcag cgaaaggaga gcaggtatga gtcagccatt attatctgtg
     3601 aacggcctga tgatgcgctt cggcggcctg ctggcggtga acaacgtcaa tcttgaactg
     3661 tacccgcagg agatcgtctc gttaatcggc cctaacggtg ccggaaaaac cacggttttt
     3721 aactgtctga ccggattcta caaacccacc ggcggcacca ttttactgcg caatcagcac
     3781 ctggaaggtt taccggggca gcaaatcgcc cgcatgggcg tggtgcgcac cttccagcat
     3841 gtgcgtctgt tccgtgaaat gacggtgatt gaaaacctgc tggtggcgca gcatcaacaa
     3901 ctgaaaaccg ggctgttctc tggcctgttg aaaacgcctt ctttccgtcg cgcccagagc
     3961 gaagcgctcg accgcgccgc cacctggctt gagcgcattg gtttgctgga acacgccaac
     4021 cgtcaggcga gtaacctggc ctatggtgac caacgccgtc tggagattgc ccgctgcatg
     4081 gtgacgcagc cggagatttt aatgctcgac gaaccggcgg caggtcttaa cccaaaagaa
     4141 accaaagagc tggatgagct gattgccgaa ctgcgcaatc atcacaacac cactatcttg
     4201 ttgattgaac acgatatgaa gctggtgatg gggatttcag accgaattta tgtggtcaat
     4261 caggggacgc cactggcaaa cggtacgccg gagcaaatcc gtaataaccc ggacgtgatc
     4321 cgtgcctatt taggtgaggc ataagatgga aaaagtcatg ttgtcctttg acaaagtcag
     4381 cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca atcagggcga
     4441 gattgtcacc ctcattggcg cgaacggggc ggggaaaacc accttgctcg gcacgttatg
     4501 cggcgatccg cgcgctacca gcgggcgaat tgtgtttgat gataaagaca ttaccgactg
     4561 gcagacggcg aaaatcatgc gcgaagcggt agcgattgtc ccggaagggc gtcgcgtctt
     4621 ctcgcggatg acggtggaag agaacctggc gatgggcggc ttttttgccg aacgcgacca
     4681 gttccaggag cgcataaagt gggtgtatga actgtttcca cgtctgcatg agcgccgtgt
     4741 tcagcgggca ggcaccatgt ccggcggtga gcaacaaatg ctggcgattg gtcgcgcgct
     4801 gatgagcaac ccgcgtttgc tgctacttga tgagccatcg ctcggtcttg cgccgattat
     4861 catccagcaa attttcgaca ccatcgagca gctgcgcgag caggggatga ctatcttcct
     4921 cgtcgagcag aacgccaacc aggcgctaaa gctggcggat cgcggctacg tgctggaaaa
     4981 cggccatgtg gtgctctccg ataccggcga cgcgctgctg gcgaacgaag cagtgagaag
     5041 tgcgtattta ggcgggtaat aacacgttga ttgatgagga gtcaaaagac tcctttgaga
     5101 caggtgacaa atgtaaaatt gcctgatgcg ctgcgcttat caggcctact gggtgagttg
     5161 caatatattg aatttgcacg atcttgtagg ccggataagg cgtttacgcc gcatccggca
     5221 tgaaacgatg agtaatctgt agagtttgat tcagaccttc tattttccgc ttatccgtgc
     5281 cccatctccc attttccctc acccacgccg tcaccgcctt gtcatctttc tgacacctta
     5341 ctatcttaca aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt catgttaccc
     5401 cgcgagcata aaacgcgtga attcgcgcat tcggtacaac aagagagata aacgatgaaa
     5461 ccgttacatt atacagcttc agcactggcg ctcggactgg cgttaatggg gaatgcacag
     5521 gcagtgacga ccattccgtt ctggcattct atggaagggg aactgggtaa agaggtggat
     5581 tctctggccc aacgttttaa cgccgaaaat ccggattaca aaattgtacc gacctataaa
     5641 ggcaactacg aacagaattt aagcgcgggg attgccgcat ttcgtaccgg caacgctccg
     5701 gctattttgc aggtttatga agttggcacc gccaccatga tggcatcgaa agccattaaa
     5761 ccggtatatg acgtgtttaa agaggcgggg attcaattcg atgagtcgca gtttgtgccg
     5821 acggtttccg gttactactc cgacagcaaa acgggccact tactctccca gccattcaac
     5881 agctcgaccc ccgttctcta ttacaacaaa gacgccttca agaaagcagg attagacccg
     5941 gaacaaccgc cgaaaacctg gcaggatctg gcggactatg ccgcgaaact gaaagcctcc
     6001 ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga aaactttagc
     6061 gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac ggacgcggtg
     6121 ctggagttca acaagccgga gcaggtgaaa cacatcgcca tgctcgagga gatgaacaag
     6181 aagggcgatt tcagctacgt cgggcgtaag gatgaatcca ccgagaagtt ctataacggt
     6241 gattgcgcga tgaccaccgc ctcttccggt tctcttgcca acattcgcga gtacgccaaa
     6301 tttaactacg gcgtaggtat gatgccttac gacgccgatg cgaaagatgc gccacaaaac
     6361 gccattatcg gcggagccag cctgtgggtg atgcagggga aagataaaga aacgtatacc
     6421 ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga gtggcatcag
     6481 aaaaccggtt atctgccaat caccaaagca gcgtatgacc tgacccgtga gcagggcttt
     6541 tatgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa gccgccgttg
     6601 ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat tgtggatgaa
     6661 gagctggaga gcgtgtggac cggtaagaag acaccacagc aggcactgga taccgccgtt
     6721 gagcgtggaa atcagttgct gcgccgcttt gagaaatcga cgaagtctta atcagtgtaa
     6781 tgtcggatgc gtttcgctta tctgacctgg catcgcgtgt aggccggata agcgaagcgc
     6841 atccggcaca gttcaggaat taaccgtaat gtcatcatcc cgtccggtgt tccgctcgcg
     6901 ctggctgcct tatctgctgg tcgcgccgca gctcatcatc accgttatct tttttatctg
     6961 gcctgcgggc gaagcgttgt ggtactcgct acaaagcgtc gatccgtttg gtttctccag
     7021 ccagtttgtc ggcctggata acttcgtcgc gctgtttcat gacagctact atctcgactc
     7081 cttctggacg acgataaaat tcagcacctt tgtcaccgtc agcggtttgc tggtgtcgct
     7141 gttcttcgcg gcgctggtgg agtacatcgt gcgcggcagc cgtttctatc aaaccttaat
     7201 gttgctgcct tacgccgtgg ctcccgccgt tgccgccgta ttgtggatct tcctgtttaa
     7261 ccccggtcgc gggctgatca cccattttct cgcggagttc ggctacgact ggaaccacgc
     7321 gcaaaacagc ggtcaggcaa tgtttctggt ggtgtttgcc tcagtatgga agcaaatcag
     7381 ctacaacttc ctgttcttct atgccgcgct gcaatccatt ccccgttcgt tgatcgaagc
     7441 cgcagccatc gacggtgcag ggccgattcg ccgcttcttt aagattgcgc tgccgcttat
     7501 cgccccggtg agtttcttcc tgctggtagt gaacctggtg tatgccttct tcgacacctt
     7561 cccggtgatc gacgccgcca cgtccggcgg gccggttcag gccaccacga cgctgattta
     7621 taagatctac cgcgaaggtt ttaccggact ggatctggct tcgtctgccg cacagtcggt
     7681 ggtgttgatg ttcctcgtca tcgtgctgac ggtggtgcag ttccgctatg ttgaaagcaa
     7741 ggtgcgttac caatgattga gaaccgtccg tggctgacga tattcagcca taccatgctg
     7801 atcctcggga tcgcggtgat cctcttcccg ctgtacgtgg cgtttgtcgc ggcgacgctg
     7861 gataaacagg ccgtctatgc cgcgccgatg acgctcatcc ccggcacaca tctgctggaa
     7921 aacatccaca acatctgggt gaacggggta ggcacgaata gcgcgccgtt ctggcggatg
     7981 ttgcttaaca gctttgtgat ggcgttcagc attacgctcg gcaaaattac cgtctcgatg
     8041 ctctcggcat ttgccattgt ctggtttcgt tttccgctac gtaacctctt cttctggatg
     8101 atttttatca ccctgatgct gccggttgaa gtacgtatct tcccgacggt ggaagtcatc
     8161 gccaacctga aaatgctcga cagctacgcc ggtttaacgc tgccgctgat ggcctcggcg
     8221 accgctactt tcctgttccg ccagttcttt atgacgctgc cggatgagct ggtggaagcc
     8281 gcgcggatcg acggcgcatc gccaatgcgc ttcttttgcg acatcgtttt tccgctctcc
     8341 aaaactaatc tggcggcgct gtttgtgatc acctttatct acggctggaa tcagtatttg
     8401 tggccgttgt tgattattac cgacgttgat ctcggcacca ccgtggcagg gatcaaaggg
     8461 atgatcgcca ctggcgaagg caccacggaa tggaactcgg taatggcggc gatgttgtta
     8521 acgcttatcc ctccggtggt gattgtttta gtgatgcagc gtgccttcgt gcgcggcctg
     8581 gtcgatagtg agaaataaga tggcaggact gaaattacag gcagtaacca aaagctggga
     8641 tggcaaaacc caggtgatta aaccactgac ccttgatgtg gcggatggcg aatttatcgt
     8701 gatggtcggg ccgtctggct gcggaaaatc gacgctgctg cgcatggttg ccgggctgga
     8761 gcgggtgacg gaaggcgata tctggatcaa cgaccagcgc gtgaccgaaa tggagccgaa
     8821 agatcgcggg attgcgatgg tgttccagaa ctacgcgctt tatccgcata tgagcgtcga
     8881 agaaaacatg gcgtgggggc tgaaaattcg cggcatgggc aagcagcaaa tagccgagcg
     8941 cgttaaagaa gcggcgcgca ttctggagct ggatggcctg cttaagcgcc gcccgcgcga
     9001 gctatccggc ggtcagcgtc agcgtgtggc gatgggtcgc gcgattgtgc gcgatccggc
     9061 ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg tgcagatgcg
     9121 ccttgagctg caacagctgc accgtcgcct gaaaacgact tcactctacg ttactcacga
     9181 tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg gcgttgccga
     9241 acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg tagcgagttt
     9301 tatcggcagc ccggcgatga acctgctgac aggccgcgtg aataacgaag gcacgcattt
     9361 cgaactggac ggcggtattg agctgccgct aaacggtggc taccgtcagt atgccgggcg
     9421 taaaatgact ctcggcattc gcccggaaca tattgcgcta agctcgcagg cagaaggcgg
     9481 cgtaccgctg gtgatggaca cgctggagat cctcggcgca gataacctgg cgcacggacg
     9541 ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga cggcaggcag
     9601 cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg aaacaggaca
     9661 acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg cggtaagctg
     9721 gccccggaaa acaccctggc ggcaatcgac gtcggggcaa aatacggtca taagatgatc
     9781 gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga cgacaatctc
     9841 gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga tttactgcgc
     9901 gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc gttgctttcg
     9961 caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat caaacccacc
    10021 actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca actgtgggcc
    10081 ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc tgcacagctg
    10141 gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga ctggcgcgaa
    10201 ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact cgataaagtg
    10261 cgagtgatgc agttgaaaga cgccggactg cggattctgg tttataccgt caataaaccc
    10321 cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga tgcgattgac
    10381 gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc tgcggcgtgc
    10441 tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc tgattcaacg
    10501 aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc agcatttgct
    10561 gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa ttattgttta
    10621 tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc tgattcagca
    10681 tccctttttg ctggatttgc tgagtctgca tctgggtttg catccgctgc tggctgggta
    10741 tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca aagccgacaa
    10801 acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct ctgaagatat
    10861 cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat ataccaggct
    10921 tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac gtttttacgc
    10981 cgggtggcca ttgctgctct gctctcagga agttgtttta gcgccgccgc cgcgcctcct
    11041 gcgccgcccg tctcgtatgg tgtggaggaa gatgtcttcc acccggtacg cgcgaaacag
    11101 ggaatggtag cgtctgtgga cgccactgcc actcaggcgg gggtggatat tctcaaggag
    11161 ggcgggaatg ccgttgatgc cgccgtggcg gtgggctacg cgctggcggt aacgcatccg
    11221 caggcaggga atctgggcgg tggtggtttt atgttaatcc gctcgaaaaa tggcaatacc
    11281 acggctatcg atttccgcga aatggcaccc gccaaagcga cccgcgatat gttcctcgat
    11341 gatcagggca acccggacag caaaaaatca ctcacttcgc atctggcttc cggcacaccg
    11401 ggtacggtag caggtttctc gctggcgctg gataaatacg gcaccatgcc gctgaacaaa
    11461 gtcgtgcagc ccgcgtttaa actggcacgc gatggtttta tcgttaacga cgcgctggct
    11521 gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag taaagctatc
    11581 ttctggaaag agggcgagcc gctgaaaaag ggcgacacgc tggtgcaggc gaacctggca
    11641 aagagcctgg agatgattgc tgaaaacggc ccggacgaat tctataaagg cacgattgcg
    11701 gaacagatcg cccaggagat gcagaaaaac ggtggcttga tcactaaaga agatttagcg
    11761 gcctataaag cggtcgaacg cactccgata agcggcgatt atcgcgggta tcaggtttac
    11821 tccatgccac cgccatcctc cggcgggatc catatcgtac aaatcctcaa tattctggaa
    11881 aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat catggcagaa
    11941 gcggagaaat acgcctacgc cgaccgctcg gaatatcttg gcgacccgga ttttgtcaaa
    12001 gtcccgtggc aggcactgac caataaagcc tatgccaaat ccattgccga tcaaattgat
    12061 atcaacaaag cgaagccgtc cagtgagatt cgccctggca agcttgcgcc ttatgagagt
    12121 aatcaaacta cccattactc tgtagtggat aaagacggta acgcggtggc ggtgacctat
    12181 acgctgaaca ccaccttcgg tacgggtatt gtcgcgggcg agagcggtat tctgcttaat
    12241 aaccagatgg atgatttctc cgccaaaccg ggcgtaccga acgtttacgg gctggtgggc
    12301 ggtgatgcca acgccgtcgg gccgaacaaa cgcccgctgt cgtcgatgtc gccgaccatt
    12361 gtggtgaaag acggtaaaac ctggctggtt accggtagcc caggcggtag ccggatcatc
    12421 actacagtgc tgcaaatggt ggtgaatagc atcgattatg gcatgaacgt cgccgaagcg
    12481 accaatgcgc cgcgtttcca ccatcagtgg ttgccggacg agctgcgtgt cgaaaaaggg
    12541 tttagccagg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc gctgaaagag
    12601 gcgatgggca gtacacaaag cattatggtt gggccggacg gtgagttgta cggcgcatcc
    12661 gacccgcgct cggtggatga tttaacggcg gggtactaag gttagcggcc ctcttcgtgg
    12721 gaagagggct attttgtcag ggcaagccga aggtagcctt ttttatttcg taatcctgta
    12781 gatattcttc cagcggcttt gccgttccca gagaatgaat ttcaccactg tctttatcaa
    12841 taataaaagg tgcgttacca gctaagcgcg cggcctcatc tccagtttcg agaaattctc
    12901 gtgcttcgaa acagaaatac cagccctggc taaagcgtcc atgtagagta atgacgaccg
    12961 ggagatctgc atcatcaagg taatggttcg ctttcgcgaa tgcgtcgtga taagtaatca
    13021 taattataat aaatattatt gttgagtgtt atattattat tcctgtgtga tacattgagc
    13081 aaagacgcgt tcatcttcgt caaagatatt attaaatgct ggttttgaaa aaagttca
//
LOCUS       DAMZII010000131        13071 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMZII010000131 DAMZII010000000
VERSION     DAMZII010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.131"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1256)
                     /locus_tag="POA77_003790"
     CDS             complement(<1..1256)
                     /locus_tag="POA77_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDI6367155.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMG"
     gene            complement(1420..1504)
                     /locus_tag="POA77_003791"
     tRNA            complement(1420..1504)
                     /locus_tag="POA77_003791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1468..1470),aa:Leu,seq:caa)
     gene            1700..2719
                     /gene="ahr"
                     /locus_tag="POA77_003792"
     CDS             1700..2719
                     /gene="ahr"
                     /locus_tag="POA77_003792"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDI6367156.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2849..4351
                     /locus_tag="POA77_003793"
     CDS             2849..4351
                     /locus_tag="POA77_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDI6367157.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4470..5552)
                     /gene="lptG"
                     /locus_tag="POA77_003794"
     CDS             complement(4470..5552)
                     /gene="lptG"
                     /locus_tag="POA77_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI6367158.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNANRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5552..6652)
                     /gene="lptF"
                     /locus_tag="POA77_003795"
     CDS             complement(5552..6652)
                     /gene="lptF"
                     /locus_tag="POA77_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDI6367159.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6919..8430
                     /gene="pepA"
                     /locus_tag="POA77_003796"
     CDS             6919..8430
                     /gene="pepA"
                     /locus_tag="POA77_003796"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDI6367160.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8564..9007
                     /gene="holC"
                     /locus_tag="POA77_003797"
     CDS             8564..9007
                     /gene="holC"
                     /locus_tag="POA77_003797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDI6367161.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9007..11862
                     /gene="valS"
                     /locus_tag="POA77_003798"
     CDS             9007..11862
                     /gene="valS"
                     /locus_tag="POA77_003798"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI6367162.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11918..>13071)
                     /locus_tag="POA77_003799"
     CDS             complement(11918..>13071)
                     /locus_tag="POA77_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDI6367163.1"
                     /translation="VFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANME
                     VNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLLLLVLLIFMAAK
                     GLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSS
                     VIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILA
                     FLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIA
                     VSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLL
                     KIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
BASE COUNT         3324 a         3354 c         3435 g         2958 t
ORIGIN      
        1 cccatgaatt tagcaaattc ataaggtgta acagagttat ttcgattggc ctcaagaaaa
       61 tcagcccacc attgaaccat taatctacgt tcgtctaaat gctcagaggt atgaatatat
      121 gctgcacgca cattgttacg ctctgtatga ctcaactgcc gttctatcgc gtcatcgctc
      181 cataagcctg attcaccaag cgcgccacgc gccatcgtcc taaatccatg cccacagacc
      241 tcagttttcg tgtcataccc catcgcacgc aaggcgctat ttactgtgtt ctcactcata
      301 actttggccg cgtcgtgatc ccctggaaaa agtaactctt tatcgccact aatctgcttg
      361 agttgctcta acaaaaccat cgcctgtcga ctaagaggaa cgatatgctc ctctttcatc
      421 ttcatgccac gatacgagta acgcacgcct ttaatttctt ctcgctttgc aggtatacgc
      481 caaagagatt tatcgaagtc gaactcatcc catcgcgcga aacgtaactc actggaacgt
      541 acaaaagtta gtaaggaaag ctcgaccgcg atccttgtca ttacacgacc acgatatgca
      601 gcaagacgtg caagaaactc agagaagcgg ctagagggta aagctggata gtgtcgtgct
      661 ttggttgtag ataacgcacc ggccatgtca ctggctggat tagagtcgat gtaatcgttc
      721 tgtacggcat aacgcataat ggccgtgacg cgctgttgca ggcgctgagc gacatcgtgt
      781 ttgccactgg catcaacttt tttaatcggg gctaacaggt ggctggtttt aagctgccga
      841 atgtcggacg aaccgatatg aggaaaaata taaagctcaa gatagcgcag aatgcgtgat
      901 cgatggtctt cactccagcg cttattactg gcatgccatt cacgagcaat tgtctcgaaa
      961 gtgtatgcgc ccgaattctc agcctgagct tctttctgct cggcttttgg gtcaatgccc
     1021 tgcactaaga gctttttagc ctcgtcacgc tttgctcttg cctgagcaag tgtcaccatg
     1081 ggccaaacac caaaagcgag gcgatcctct tttttgtcag aggggcgtct gtatttcatg
     1141 cgccagtatt tagaaccctt agccgaaacc tcgagataca aaccgccgcc atcggccatt
     1201 ttgtaggttt tctcttttgg ctttgctgtc tcgacctgtc tggcgttgag cttcatttgg
     1261 gggcacattt ctaatcgaag ttaagatgcc cccaattatg cccccaatag catccggatt
     1321 tcaacggact agctcggaca gctcaggaca gaaaaaccgc tacaggcatt gattttaaag
     1381 ggtttgctgg attttctcgg atggtcttgg aagtactaat ggtgccgaag gccggactcg
     1441 aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat ttcgccactt
     1501 cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg ccaccatgca
     1561 agcgccagct tgctcgcacc tcgctaagtg ctgaaatttt cagcattgca tacagcgatg
     1621 tgtaaccttt gtcacactcc aggcaccccg ccctgccatg ctctacactt cccagacaac
     1681 accagagaag gaccaaaaaa tgtcgatgat aaaaagctat gccgcaaaag aagcgggcgg
     1741 cgaactggaa gtttatgagt acgatcccgg tgagctgaag ccacaagatg ttgaagtgca
     1801 ggtggattac tgcgggatct gtcattccga tctgtcaatg atcgataacg aatggggatt
     1861 ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg cgctcgggag
     1921 cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct ggacggcgcg
     1981 tagctgtggt cactgcgacg cctgtattag cggaaatcag atcaactgtg agcaaggtgc
     2041 ggtgccaaca attatgaatc gcggcggttt tgccgagaag ttgcgtgcag actggcaatg
     2101 ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgc tgtgtggtgg
     2161 tatcacggtc tttaaaccac tgttgatgca ccatatcaca gccaccagcc gcgttggggt
     2221 aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa tgggatgcga
     2281 ggtgacagcc tttagttcga atccggcgaa agagcaggaa gtactggcga tgggtgccga
     2341 taaagtggtg aatagccgcg atccgcaggc actcaaagca ctggcggggc agtttgatct
     2401 cattatcaac accgtcaacg tcagcctcga ctggcagcct tattttgagg cgctgaccta
     2461 tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc cggcctttac
     2521 gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt atgagctgcg
     2581 taagctgatg cgctttgccg cccgcagcaa ggttgcaccg accaccgaat tgttcccgat
     2641 gtcgaaaatt aacgacgcca tcaagcatgt gcgcgacggt aaggcgcgtt accgcgtggt
     2701 tctgaaagcc gacttctgat ccctttgccg ggtgcctgtc gcccggcgtt taaatctctt
     2761 tggaaggcgt ctcgcaatct gctgaattta gcggtgaaga gtgcgcagcc gttgcctata
     2821 ctccaggcag gaaactggag gatatctcat gagcgaaccc ctgttaattg cccgcacgtc
     2881 ggacacagaa ctgtttttat tgccgggaat ggcgaatcgt cacgggctga ttactggcgc
     2941 aacggggacg ggtaaaactg tcacgctgca aaaactggcg gagtctctat cggaaatcgg
     3001 tgttccggtg tttatggccg atgtgaaagg cgatctgacc ggcatcgcgc aggcaggaac
     3061 ggcatcggaa aaactactcg caaggcttaa aaatatcggc gtcaatgact ggcaaccgca
     3121 tgctaatccg gtggtggtgt gggatatctt tggcgagaaa ggccatccgg tgcgggcgac
     3181 ggtttcggat ctggggccgc tgttgctggc gcggctgttg aatctcaacg atgtgcaatc
     3241 tggcgtgctg aatatcatct tccgtattgc tgacgatcag ggattgttgc tgctcgactt
     3301 taaagatctg cgggcgatta cccagtacat cggcgataac gccaaatctt tccagaatca
     3361 gtacggtaat atcagtagcg catcggttgg tgccatccag cgcggactgt tgtcgctgga
     3421 acagcaaggc gcagcacact tctttggcga gccgatgctg gatatcaaag actggatgcg
     3481 caccgatacc aacggtaaag gcgttatcaa tatcctcagc gccgagaagc tttatcagat
     3541 gccgaaactg tacgccgcca gcctgctgtg gatgctttca gagttgtatg aacaattgcc
     3601 ggaagcaggc gatctggaga aaccaaaact ggtgtttttc ttcgacgagg cacatctgct
     3661 gtttaacgat gcaccgcagg tactgctgga taagattgag caggtgatac ggcttattcg
     3721 ctcaaaaggc gtaggcgtct ggttcgtttc gcaaaacccg tctgatattc cggataatgt
     3781 gctcgggcag ctcggtaatc gcgttcaaca cgctttgcgt gcttttacgc ccaaagatca
     3841 gaaagcggta aaagctgcgg cgcaaaccat gcgggccaat ccggcatttg ataccgaaaa
     3901 ggcaatccag gaactgggga ccggcgaggc gttaatctcg tttcttgatg tgaaaggaag
     3961 tccttcagtg gtggaacggg cgatggtgat tgcgccttgt tcgcggatgg gaccggtgac
     4021 ggaagatgag cgtaatggct tgattaatca ctctccggtg tatggcaagt acgaggatga
     4081 ggtggaccgg gaatccgcct atgagatgtt gcaaaaaggc gtacaggcaa gcgccgagca
     4141 gcaaaataat ccccccgcga aagggaaaga ggtggcggtg gatgacggca ttcttggtgg
     4201 attaaaggat attttgtttg gcactaccgg accacgcggc gggaagaaag atggtgtggt
     4261 gcaaacaatg gcgaaaagcg ccgcccgcca ggtgacgaat cagattgtac gcgggatgtt
     4321 ggggagtttg ctggggggaa gaagaaggta agtcaggtgc ggtctgtgcc cctcacccta
     4381 accctctccc caaaggggcg aggggaccga tcgaactcgt atttgacttt atcaacaatg
     4441 tgaaagggag ctttcgctcc cttgcttggt tacgattttc tcattaacag ccacaggctg
     4501 attaagaaga agctggcgct tggcaacagt gcgccgatga tcggcgggat gccataaacc
     4561 aacgtcaacg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa gctgataccg
     4621 gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagatgaa cgacagcgcc
     4681 atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat gttgagctga
     4741 taacgtccag cgtcctgacc gctcgacttc agatacttca cgtagttgtg caaaccgctg
     4801 attgagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt gaggttggtt
     4861 ttccaggtgc cgctcaccgt ctgcgaaccg gtgatctgtt tcggattggt cagatcagat
     4921 tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc agtagcggca
     4981 tagcgtacgg attgcagacg acgatttgcg ttaaaggcat aaatgctgat gccacctaac
     5041 tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt tgccatcttt cgcccataag
     5101 ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta gttacgcgcc
     5161 atctgctcgc cctgcggcgc gacccattca ccaatcgcca tcgtcagcaa gactagcgga
     5221 atggcggttt tcatcaccga cagcgcaacc tgcatacggg tgaaaccaga agcctgcatc
     5281 accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc aagcagagct
     5341 gccatcggga agaagatttg cacatctttc ggcacgctca gcaaggtata cattcctgcg
     5401 cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa cttgataatg
     5461 cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat agttttaccg
     5521 atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc gaaaaactgg
     5581 cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc aaagccagat
     5641 aaatcaggtt aacggtccac atccacagcg tcgggtccag cttaccttta ccgccgttcg
     5701 atttcaggga ggtctggatc aggaagaaca acagatacag cagcatggct ggcagcatcg
     5761 atagtacgcg cccctgacgc gggttaacca cgctcagcgg tacgaccata agtgccatca
     5821 taaacacggt gaacaccaac gtgatacgcc agttcagttc tgcgcgagca cgatcggtgt
     5881 cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg agcgccaccg
     5941 cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa tcacgtaaca
     6001 gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag ccgtcgcgca
     6061 gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta ccttttggtc
     6121 gaatttgcgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg atgaacagca
     6181 ccgaactgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc atgccagggt
     6241 tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt cccgcccaca
     6301 tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc gctttcacca
     6361 gaaccgcttt gctcaggccg caggcatgca ttaccgtaat ttcactttcg gtatacagtt
     6421 tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc tgcgccattt
     6481 ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg ccgtcaaccg
     6541 ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag atgaagagta
     6601 tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc actttaaata
     6661 cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta aacgtcattt
     6721 attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat tcacctctca
     6781 gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa gtcatcgcaa
     6841 cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg ttgtctttaa
     6901 gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga gaaacagcgg
     6961 agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat tgcagaacag
     7021 ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gtcggggcga actggaagga
     7081 aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga gcgaattctc
     7141 cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca ggttattcag
     7201 aaaaccatta atacgctgaa tgatactggc tcaatggaag cggtctgctt tctgactgaa
     7261 ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga aacggcaaaa
     7321 gagacgctct acagtttcga tcagctgaaa acgaacaaga gcgaaccgcg tcgtccgctg
     7381 cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga gcgcgcgatc
     7441 cagcacggtc tggcgattgc cgccgggatt aaagcagcaa aggatcttgg caatatgccg
     7501 ccgaatatct gtaacgccgc ttacctcgct tcacaagcgc gccagctggc tgacagctac
     7561 agcaagaatg tcattacccg cgttatcggc gaacagcaga tgaaagagct ggggatgcat
     7621 tcctatctgg cggtcggtca gggttcgcaa aacgaatcgc tgatgtcggt gattgagtac
     7681 aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg tttaaccttc
     7741 gactccggcg gtatctcgat caagccttca gaaggcatgg atgagatgaa gtacgatatg
     7801 tgcggtgcgg cagcggttta cggcgtgatg cggatggtcg cggagctaca actgccgatt
     7861 aacgttatcg gcgtgttggc aggttgtgaa aacatgccag gcggtcgcgc ctatcgtccg
     7921 ggcgatgtgt taaccaccat gtctggtcaa accgttgaag tgctcaacac cgacgctgaa
     7981 ggccgcctgg tactgtgcga cgtgttaact tacgttgagc gttttgagcc ggaagcggtg
     8041 attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca cattactggt
     8101 ctgatggcga accacaatcc gctggcccat gaactgattg ccgcgtctga acaatccggt
     8161 gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacaactgga gtccaatttt
     8221 gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg ttgcttcctg
     8281 tcacgcttta cccgtaagta caactgggcg cacctggata tcgccggtac cgcctggcgt
     8341 tctggtaaag cgaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca gttcctgtta
     8401 aaccgcgccg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc ctaaccctct
     8461 ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc ttttagggtg
     8521 aggaatgcag caatatttaa atccaccaca agaagcccca tttatgaaaa acgcgacgtt
     8581 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc agctggtgtg
     8641 tgaaattgcc gcagaacgtt ggcgcagtgg taagcgcgtg ctcatcgcct gtgaagatga
     8701 aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc
     8761 gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg cctggccgca
     8821 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     8881 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     8941 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     9001 gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta cgagcactgg
     9061 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     9121 atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
     9181 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
     9241 actgaccacg cagggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
     9301 ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg ggaatggaaa
     9361 gcggaatccg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc cgtggactgg
     9421 gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
     9481 cgtctgtata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg ggatccgaaa
     9541 ctgcgcaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
     9601 cacatccgct atccgctggc tgacggcgcg aaaaccgcag atggtaaaga ttatctggtg
     9661 gttgcgacta ctcgtccgga aaccctgctg ggcgataccg gcgtggccgt taacccggaa
     9721 gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt taaccgtcgt
     9781 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaagatc
     9841 accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct gccgatgatc
     9901 aacatcctga cctttgacgg cgatatccgt gaaagtgccc aggtgttcga taccaaaggt
     9961 aacgaatctg acgtttattc cagcgagatc cccgcagaat tccagaaact ggagcgtttt
    10021 gctgcacgta aagcggtcgt tgctgcggtt gacgcactcg gcctgctgga agaaattaaa
    10081 ccacatgacc tgaccgttcc ttatggcgac cgtggtggcg tggtcatcga accgatgctg
    10141 accgaccagt ggtacgtgcg tgccgatgtc ctggcgaaac cggcagttga agcggttgag
    10201 aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc ctggatgcgc
    10261 gatattcagg actggtgtat ctctcgtcag ttgtggtggg gtcaccgtat cccggcatgg
    10321 tatgacgaag cgggtaacgt ttatgttggc cgcaacgaag acgaagtgcg taaagaaaat
    10381 aacctcggtg ctgatgttgt cctgcgtcag gacgaagacg ttctcgacac ctggttctct
    10441 tctgcgctgt ggaccttctc tacccttggc tggccggaaa ataccgacgc cctgcgtcag
    10501 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
    10561 atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
    10621 cacactgttt acatgaccgg tctgattcgt gacgacgaag gccagaagat gtccaaatcc
    10681 aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    10741 gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    10801 gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg cttcaccctg
    10861 gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgcct ggaaggttac
    10921 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacagaaggt
    10981 caggattgcg gcttcaacgg cggcgaaatg acgctgtcgc tggcggaccg ctggattctg
    11041 gcggagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    11101 atcgccgcag gcattctgta tgagttcacc tggaaccagt tctgtgactg gtatctcgag
    11161 ctgaccaagc cggtaatgaa cggtggcacc gaagcagaac tgcgcggtac tcgccatacg
    11221 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    11281 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    11341 cagccgttcc cgcagtacga tgcatctcag gttgatgaag ccgcactggc cgacaccgag
    11401 tggctgaagc aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa catcgcgccg
    11461 ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat
    11521 gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    11581 gatgacaaag gtccggtttc cgttactaag atcatcgacg gcgcagagct gctgatcccg
    11641 atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga agtggcgaag
    11701 atcgaaggcg aaatcagccg tatcgagaac aagctggcga atgaaggctt tgtcgcccgc
    11761 gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc agaagcgaaa
    11821 gcgaagctga ttgaacagca ggctgttatt gccgcgctgt aatcaaaaca cagtaatgaa
    11881 aacgaaggcc ggagcatgct ccggcctttt ttatctctta cagaaatggt aaagaaggca
    11941 taattccccg tgaaatcctg gctaagaagc ctttatctgg ttgttcttct ttattgatta
    12001 aaggaaggtc atccaaatcg cccagcaaat acgtattttt tgcctgccag tcaatcatcc
    12061 atattttcag cagtggataa gccagaccgc ccgtaatccc ggatatcacc accaacgcac
    12121 acatgcgata aagcataccg tggtacgtta aagttgagcg aaaacgaata cgcccatcat
    12181 tcagtgacag gttgctcata aaatgatttc gcaacgacac cgttaaataa ctggtgctaa
    12241 cagcaatccc aaaataatag attaactgcg cgattatcat tttacgttgc atttccataa
    12301 attgctgtaa attctcaata tcatcatttg catacacaga actatcatat gcatttaata
    12361 tttgatcgaa gataatataa ccagcaacag cgagaaaagg taataaagct aaaaatgcga
    12421 gaatggcata ttttatacaa tacgtagtat ctaatttcac cttgaaacga tgtataccga
    12481 aactggtatt gctccagaga aaactcatta ccaggctata taaagtacca ttaaaaatac
    12541 caatggaaac aataccaact attgccatca gaaccacaga tataataaca ctacttgaac
    12601 tattggcatg tagcatcttt gtcgagataa agaaaacagt ccccatccca atggccatta
    12661 aaatcggcaa gaaaaaggtt acccaccaga accctttcat agagcaatta aaactaaatc
    12721 ttacgccgtt gagactggtc atcaaggcct gataacgcag tccttttgct gccataaata
    12781 taagcaaaac caacagtaac aaagtcaaaa cacagccaac aagtggcata tctgctgaca
    12841 ctgtcattaa gattgcgaaa tagcaaaaaa caaaaacaag acaactaaca aaaacattcc
    12901 caccagtaat tccataagaa aatcgttgtc cgttaacttc catattagca tagagataac
    12961 gcttacattt cattaatgcc catggcaaat agatacctag cgttataatc gttaacaaca
    13021 cattcaccac acaaataaga aaatatctct cacctgtacc atgaaaaaca a
//
LOCUS       DAMZII010000132        13046 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZII010000132 DAMZII010000000
VERSION     DAMZII010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.132"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            403..1092
                     /gene="mutH"
                     /locus_tag="POA77_003800"
     CDS             403..1092
                     /gene="mutH"
                     /locus_tag="POA77_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDI6367164.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1161..1874
                     /locus_tag="POA77_003801"
     CDS             1161..1874
                     /locus_tag="POA77_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6367165.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2012..2230
                     /gene="ygdR"
                     /locus_tag="POA77_003802"
     CDS             2012..2230
                     /gene="ygdR"
                     /locus_tag="POA77_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDI6367166.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2338..3378
                     /locus_tag="POA77_003803"
     CDS             2338..3378
                     /locus_tag="POA77_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDI6367167.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="POA77_003804"
     CDS             complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="POA77_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDI6367168.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4596..6755)
                     /gene="aas"
                     /locus_tag="POA77_003805"
     CDS             complement(4596..6755)
                     /gene="aas"
                     /locus_tag="POA77_003805"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDI6367169.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7341..8372
                     /gene="galR"
                     /locus_tag="POA77_003806"
     CDS             7341..8372
                     /gene="galR"
                     /locus_tag="POA77_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDI6367170.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="POA77_003807"
     CDS             complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="POA77_003807"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI6367171.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9763..10698
                     /gene="lysR"
                     /locus_tag="POA77_003808"
     CDS             9763..10698
                     /gene="lysR"
                     /locus_tag="POA77_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDI6367172.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="POA77_003809"
     CDS             complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="POA77_003809"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDI6367173.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11506..12924)
                     /gene="araE"
                     /locus_tag="POA77_003810"
     CDS             complement(11506..12924)
                     /gene="araE"
                     /locus_tag="POA77_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDI6367174.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMATLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3307 a         3509 c         3209 g         3021 t
ORIGIN      
        1 gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt
       61 tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg
      121 aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag
      181 gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc
      241 agtgaccaga tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa
      301 aagcaatata aatcagagaa ttgaacaacg catgtggaaa aagttacact gcgaatattc
      361 ggcacataat tgctgtttgt tttttaacca aggtgtcatg acatgtccca acctcgccca
      421 ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt
      481 tatacattgg gagaactggc ggcacttgcc gggctggtta cgccagagaa tttaaaacgc
      541 gataaaggct ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa
      601 cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt
      661 ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggggtg
      721 acctgggaaa ccagccacgt gcgccacaaa ctcaaacgcg tactgtggat accagttgaa
      781 ggcgagcgca gcatcccgct ggcgcagcgt cgcgttggat caccgttgct gtggagcccg
      841 aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga tatgattgtt
      901 ctcggtcagg ttgagcggat taccgctcgt cacggggaat atttacagat acgaccgaaa
      961 gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg
     1021 ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cgctactggc ccgtcatttt
     1081 ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt
     1141 tttttggcag ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg
     1201 cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt
     1261 ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg
     1321 cgggagccat ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga
     1381 caaatccgct ttttacaata ttcagtcagg aaatttccgc ccgtgatttg attctgcttc
     1441 tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg
     1501 aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc
     1561 tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc
     1621 tgtttattat gatggccgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct
     1681 cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga
     1741 ttctggtcgg ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca
     1801 tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa
     1861 agaacccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tcctgttacc
     1921 ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata aacagactat
     1981 tatcataggt gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc
     2041 agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga
     2101 tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag
     2161 ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat
     2221 tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt
     2281 cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg
     2341 caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg
     2401 acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct
     2461 cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg
     2521 caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag
     2581 ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg
     2641 gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgccta
     2701 cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac caactgcttc
     2761 ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct ggatacgctg
     2821 gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa
     2881 actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt
     2941 gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa
     3001 gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg
     3061 accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg
     3121 ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc
     3181 agacgtcatg gcctggaccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt
     3241 gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt
     3301 ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat
     3361 acttatccgg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
     3421 gctgccagat ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa
     3481 tgggcacgac cgggatgcct atcattaccg ccagcgagta aatgcccagc atcaacaaca
     3541 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
     3601 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc atcacgccaa
     3661 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggaaaaa atcagtacca
     3721 ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaactaact
     3781 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtgg
     3841 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
     3901 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
     3961 tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
     4021 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
     4081 cgtaggccag tgcgcatgcg gccagggcga cgaggacatg ccagtcagcc agcacaccac
     4141 cggctacgga accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt
     4201 tcactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
     4261 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
     4321 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
     4381 tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt
     4441 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
     4501 acagtagggc attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
     4561 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg
     4621 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
     4681 ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac
     4741 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
     4801 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
     4861 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
     4921 cggccctgaa tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac
     4981 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc
     5041 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
     5101 tcttcgatac cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc
     5161 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ctcggttaca
     5221 ccatagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
     5281 tgtagttttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
     5341 aaacgcgcgt agtgaccgag gaaagtcgag gtaccgaaca acacggtgca actgcggtca
     5401 tacaccaact ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacttctgca
     5461 cctgtaagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaat
     5521 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacgttc
     5581 gccagaatgc ttttatggct atggacgacg ccttttggat ggccttcaga accggaggta
     5641 aagaggatca gcgcctcttc ttccggctgc tgttttacct gcgccaggcg cggcatcagc
     5701 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
     5761 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
     5821 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagcccc
     5881 tttaccccag cagtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa
     5941 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
     6001 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
     6061 tctggggtaa aattgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgca
     6121 ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt
     6181 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
     6241 atctccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
     6301 accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata
     6361 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
     6421 cctgtagtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
     6481 cgtaccagat gtttaatagc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
     6541 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
     6601 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
     6661 attagaacgc gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa
     6721 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
     6781 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
     6841 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
     6901 cgcataatct caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt
     6961 cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt
     7021 tactgttaca gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa
     7081 ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag
     7141 caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca
     7201 aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg
     7261 aaaacacgcc accccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt
     7321 ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt
     7381 cagtcgccac cgtttcccgc gtcattaata attcacctaa agccagtgaa gcttcccggc
     7441 tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc
     7501 tggcgcagca gaccactgaa acggtcggtc tgatcgttgg tgatgtttcc gatccgtttt
     7561 tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga
     7621 ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc tattgagcaa ctgatccgcc
     7681 atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat
     7741 taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc
     7801 gttgtattgc tcttgacgat cgttacggtg cctggctggc aacgcgtcat ttaattcagc
     7861 aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc
     7921 gtctgcaagg gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg
     7981 tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac
     8041 gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg
     8101 gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg
     8161 atgtgctggt gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga
     8221 cgatggcgac ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg
     8281 aaatcactaa tgtctttagt ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc
     8341 tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt
     8401 aattcttcga tggtctgacg acggcgaatc aaccgcgcct gaccattatc aaacagaact
     8461 tctggtaaca gcggacggct attgtagttg gatgacattg atgcgccata tgcccctgta
     8521 tcatgcagta ccagataatc acccgccttc acttccggca aggcgcgggt ttcaacattt
     8581 cccccttcct gctgggtaaa gacatcgccc gattcacata atggcccggc gacgacggtt
     8641 tccaccgttg gcgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg
     8701 taactaccgt acattgccgg gcgcatcaga tcgttgaacc cggcatcaac cagcacaaag
     8761 tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaacacgcc agactgcgct
     8821 accagaaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgagcgatt
     8881 tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc
     8941 tgttgataag gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat
     9001 tcgatgacct gacgtaccat agcaccacac acctgttcca gatgggcata atcaacgcca
     9061 gaaccaatgt gcatgtgaat gccgaccagc tgcaaatgat gacgttgtat cacgtccagt
     9121 gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt
     9181 ttttggctat gtccgtgacc aaaccccggg ttaacgcgca gccatacccg atgccctggc
     9241 gaaacctggc ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat
     9301 tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg
     9361 gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg
     9421 gaatccactt tcacgccctg ctcacgcatt aatcgcaaaa tatgaatatt ggaacaggct
     9481 ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt
     9541 tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg
     9601 gcggtgagat cggtatcggt gctgaacagt gaatgtggca taacaaactc cagatgagtg
     9661 cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct
     9721 atgcaacaat gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta
     9781 cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac
     9841 ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc
     9901 gggctgaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt
     9961 ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa
    10021 agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa
    10081 tcttttttac cgcagctcct gcaacccttt ctggtacgtt atcccgatgt cagcttaaat
    10141 atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta
    10201 ggactcactg agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta
    10261 gatgaagtgt gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg
    10321 gatgattttc agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg
    10381 ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc
    10441 gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc
    10501 accgcactgg attatgcggc aagcggttta gtggtgcggc ggttcagtat tgcggttccg
    10561 ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt
    10621 agtgagcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc gattttgtcg
    10681 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgcg gtatcaaaca
    10741 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
    10801 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
    10861 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
    10921 catccgcttc aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa
    10981 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
    11041 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
    11101 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
    11161 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
    11221 gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
    11281 gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt
    11341 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac
    11401 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta
    11461 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt
    11521 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt
    11581 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
    11641 gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat
    11701 attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg
    11761 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
    11821 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
    11881 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
    11941 tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa
    12001 cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc
    12061 cgccattttg aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg
    12121 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
    12181 cagtgcccaa ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
    12241 cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc
    12301 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
    12361 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt
    12421 atcggataaa aacgccagca cgatgccgag tgtggccatc aactggtaca tactgatcat
    12481 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc
    12541 gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc
    12601 aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt
    12661 acgacccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
    12721 catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa
    12781 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
    12841 agctaccgaa acaaacatat tcatacgccg cgtatcccgc aaagaacgtg gcgttaaagc
    12901 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
    12961 aatacgtgaa caactcacgc aggtgtcagg tcggaaacag cataaatatg gattaaattg
    13021 ctgcgacatg tcgttatgtg atggat
//
LOCUS       DAMZII010000133        13005 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZII010000133 DAMZII010000000
VERSION     DAMZII010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.133"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(207..1745)
                     /gene="cadC"
                     /locus_tag="POA77_003811"
     CDS             complement(207..1745)
                     /gene="cadC"
                     /locus_tag="POA77_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDI6367175.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(2362..2437)
                     /locus_tag="POA77_003812"
     tRNA            complement(2362..2437)
                     /locus_tag="POA77_003812"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2402..2404),aa:Phe,seq:gaa)
     gene            complement(2544..3119)
                     /locus_tag="POA77_003813"
     CDS             complement(2544..3119)
                     /locus_tag="POA77_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6367176.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3156..4853)
                     /gene="dsbD"
                     /locus_tag="POA77_003814"
     CDS             complement(3156..4853)
                     /gene="dsbD"
                     /locus_tag="POA77_003814"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDI6367177.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAVGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4829..5167)
                     /gene="cutA"
                     /locus_tag="POA77_003815"
     CDS             complement(4829..5167)
                     /gene="cutA"
                     /locus_tag="POA77_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDI6367178.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5283..6584)
                     /gene="dcuA"
                     /locus_tag="POA77_003816"
     CDS             complement(5283..6584)
                     /gene="dcuA"
                     /locus_tag="POA77_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDI6367179.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6702..8138)
                     /gene="aspA"
                     /locus_tag="POA77_003817"
     CDS             complement(6702..8138)
                     /gene="aspA"
                     /locus_tag="POA77_003817"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDI6367180.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8475..8951
                     /gene="fxsA"
                     /locus_tag="POA77_003818"
     CDS             8475..8951
                     /gene="fxsA"
                     /locus_tag="POA77_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDI6367181.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(8967..10223)
                     /gene="yjeH"
                     /locus_tag="POA77_003819"
     CDS             complement(8967..10223)
                     /gene="yjeH"
                     /locus_tag="POA77_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDI6367182.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            10499..10792
                     /locus_tag="POA77_003820"
     CDS             10499..10792
                     /locus_tag="POA77_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6367183.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10836..12482
                     /gene="groL"
                     /locus_tag="POA77_003821"
     CDS             10836..12482
                     /gene="groL"
                     /locus_tag="POA77_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6367184.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12620..12973
                     /locus_tag="POA77_003822"
     CDS             12620..12973
                     /locus_tag="POA77_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDI6367185.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
BASE COUNT         3412 a         3129 c         3289 g         3175 t
ORIGIN      
        1 gttttttatt aacacatcag gatcgcaagt tgatatcatg aaaagataaa cattcaatgt
       61 ttacaatgga ttgcgggata ttctctggtt aaatttatgt aataaaaatt atgcggcaaa
      121 taaattgccg caacatatta taccaacaga aacatacaaa aactcaacaa caaatattcc
      181 cgagcataaa tcaacccggg agttacttat tctgaagcga gaaatttgtc gagataaggt
      241 acaacataag gaacagaagt ctggaatata ccattttcaa tccagtaaag ggtgtttgcc
      301 cctgggcgta aattaaaggc ggtgagatat gcatcagccg cttcccggtt catccccttc
      361 atttcataaa ccttgccaag caacacataa tttagccagg acatttcaag atcaatgcca
      421 gtatttatcg cctggtaaga ctcatctgtt ttacctttta ccagagcact gaccgctttt
      481 atttgatata taatggacag gttattcaat tccggcagtg taacaatgtt gtctatttct
      541 gtgttcagtg ctgctaattg tttttcatct aaaggatgtt gagaatggcg cacgatatca
      601 actaatgctt tttctgctct cgcgtaggta aattctgggg atgattgaac aatctcacct
      661 aataattcac tggcacgatt caatgattta tcatcgccat gcagtaaata atcatgtgcc
      721 tgataaaaat tagttaataa cgcaccacga tgaggcaata tttgctggag cgtctcctgc
      781 attcgttgtg gccacggttg gtttaacgct tttgataaac tctccagtaa atcattttga
      841 atcgccagct gattaccgtt agtgatgaca taacgtttat ccagcatggt tgaaccatct
      901 gcattgtcta ccaattttat cgacataaag cattgttgag cacggtattg gcgctgatta
      961 acaaacgcaa tagataatgt tttaccggaa ctgctcggtt catcaatgtt gtagttgatt
     1021 ttgtcatgca ccataaaggt ggagaaggtg ttaagtgatg tcgccaccaa atcacccacg
     1081 cctatcgcgt aagagagctg atacggggaa ctccagctgt tacaactttt atttaccata
     1141 ttaatgtcaa tatcgcgtgg attgagcaaa atacgcgatt tgctcatagg aagacgtgta
     1201 tcaagagttg aaaacgctac cagtgctaca cagataccta acgacaacag gaaaaaaaac
     1261 catacccaaa aggtagtgaa tcgtttgctt ttaactgggg attgttcagg tggcgttgcg
     1321 gtgttttgaa tgttaagact gtgggaggga gaatctgtgg caggaaccgc ctctggtata
     1381 gggggaggcg aagatagcat tatttcctct ccctcttctt cgctgtacca gataaccggc
     1441 accattaatt tatagccgcg ctttggtaca gtagcgatat agacaggact atcttcatca
     1501 ttatctttta atgacttacg tagttctgag atactctgcg tcacaacatg attggtgaca
     1561 atacttctct tccagacatt atcgataagt tcatccctgc taagtacttc gccactgtgt
     1621 tgagcaaaga aaaccagaag atcgattaat ctcggctcaa gggtaagttg acgcccattg
     1681 cggctaattt ggtttatgga cggagtaaca agccattcgc caacgcgaac tacaggttgt
     1741 tgcataatag aaactcattc gaaaagggaa tgatgcaatg ataattgcca caacctattt
     1801 ttaccatcta tagatgggtt tatttacata ttattggtga atgcaagacg ttatttttac
     1861 cagccataaa cttcttgatt acatagtatt acgaaaggat tttactgaga accagaagta
     1921 atattcctta ccatcaaaat tcatcatctt tgccaaagaa aaatgttcag aaaataatcc
     1981 atggaaaatt gtccggagcg cttactattt taatggattg ttagtctttg catgagcaag
     2041 cggactgata catttctctt tgttcttatt cagaaaatct catcagtcgg cgttcagacc
     2101 ggtgttgtgc tttatggatg cgttacgctc ctgatgacgt catttgacgt tcaacaacat
     2161 cacggggccg cacgacattt cacgtcagtt agtgctatcg ctcaggaaca aattttcccg
     2221 aattgggata tgcccgcaaa ttgctggtga tgtggggaga atctggttga gttcgggaga
     2281 attgatttgg aggcagaacg cttaaattgt ggcgtccaga aacgaaaaac ggacctccgt
     2341 ggaggtccgt tgatatgaat ttggtgcccg gactcggaat cgaaccaagg acacggggat
     2401 tttcaatccc ctgctctacc gactgagcta tccgggcaac ggggcgcatt aaaccgtaat
     2461 ctgcacatct cgtcaaccta atttcagtaa aagcgattca actgcttaag attgcggcaa
     2521 atcgcttctt ttctgtgttt ctgtcaggtc aatgcaccac cctggcggca gcgggcgaag
     2581 cgcaggatat cttccgccag ccgatgtgca gtgtcgacat ccgcctggct acgattcacc
     2641 agcattcggc ttaaacagcc ttccagcacc agttccatct gctttgctac catcgccgga
     2701 tcgtcaactt ccagtgtggt taacagttcg tgggtgaaat cgtaggccgc gcttttttgc
     2761 tgatcggcca gttgatgaat agggtggcca ggatcgggat aaaacgtaca ggcagcgata
     2821 aatagacagc ccggatagcg gttgttttta acgcactccg ataacgcctg ataacgtgcc
     2881 agcagctttt gttcggcggt ttgcgtttcg tccagcatca gctgacgacg ccagacatct
     2941 atctgttggc taagataacg cagcgcatcg tagaggattg cctctttgtc tggccagaag
     3001 cggcgtagct cgtccagtgg ataatccaca cgttcagcaa ccatctccag cgtggtgttg
     3061 gcaatccctt gtaattctaa taatttcagg gcttctccca gtacatcttc acgttgcacg
     3121 ctattttcct ccgtctttcc cactgcaagt gtcgttcacg gttggcgatc gcgcaaatgt
     3181 gcgctgaagg tttcagcatc cataaagccc gtgacgcgtg cttgtggatg ctcctggcct
     3241 tgtccgtcaa aaaagagaat tgtcggtagg ccaaggacat taagatgctt taacagcgcc
     3301 acatcttgtg cgtcgttggc cgtgacgttg gcctgaagta agaccgtgtc tgctaacgct
     3361 ttttgcacct gcgggtcgct gaaggtgtat ttctcaaact ctttacaggc gacgcaccag
     3421 tcggcataaa gatctaacat caccggtttg cctttggctt caacgagcgc ctgatttaac
     3481 tcatctaccg ttttgatttg tgtaaagttg agatgcgtct gagtttgcgc ggtatgcgtc
     3541 gcaccaaatg cccaatcctg aagtgggcgc acgctaacca atgccgctgc cagcaggatt
     3601 atttgcacca cgcgcatcca gccgcgttta gcctgtaggc tggtgataaa ggcccagcca
     3661 aagaatgcga cacccagcgc cgaccacaag cgtaatcccc atacatcacc aatcactcgc
     3721 tccagcagga agaccggcag tgcgaggatc acaaaaccaa acgcggtttt gacttgttcc
     3781 atccacgggc cgcttttcgg cagcaagcgg ttaccaaaga cggtaattag catcagcggc
     3841 aggcccatgc ccaacgcata gagataaagc gtgccgccgc ccagccacat gttcccgctt
     3901 tgggcgatat acagcagaat cgcgctaagc ggtgcggtgg tgcatggtga acagatcagt
     3961 ccggcaatcg cccccataac aaacacaccg ccaggtgagc cgccctgttg gcgattgctc
     4021 atcaacgtga gacgtgtttg cagcgaagag gggagttgta gagtaaacaa gccaaacatt
     4081 gacatcgcca gcaaggtaaa gacgatggcg aggccaatga gcacgtatgg gtgctgtagc
     4141 gccgcctgga actgtaaccc tacggcggca actaccagac ccagcgccgt gtaagtcagt
     4201 gccatcccct gcacataaat aaaggtcagc aacaatgctc tggcagtgga gagccgctgt
     4261 ttaccaccca gcacgatgcc agaaatcagt gggtacattg gcagcacgca tggcgtaaag
     4321 gcgataccaa taccgatcaa caacgcccag agcgcggaaa agggcaattg cgcggtgtgc
     4381 tgctcctgct gcgaaacaga cacaggctgt gacgctgcgt tgttggcgac cacttcgctt
     4441 aacggaacgg ttttggtttc tggcggataa cagaaaccgg catcagcaca gccctggtag
     4501 gtgacagtta acgtcgctcc cgcactcgcc tggttgatgg ttacgggaag cgtcagccga
     4561 tcgcggtaaa tctcgctttt gccgtaaaac tcatcttcat gccagacgcc ttgcggcagc
     4621 tgcacgtcgg caattttcgc gtgttccggc gtaatgcgga tctgtttacg gtagaggtag
     4681 taaccgtctt tgatctgcca ggtcagattc aggtcatgtt ggttttgctg aaaatcaaaa
     4741 gcaaaggctt gatccgcggg gacaaattgt gaacgtcccg gcgcgtcgaa taatccggca
     4801 aaaacggaag tgctgcaaag tagcaggatc agcgtaaaga tgcgttgagc catgagaggt
     4861 aatctgtgtc tccgtgtgta acaggtaaaa ccagaagttc cggggtttga tatggatgat
     4921 gagacttcag gcattccagc agtgcctgct ggtgagatac ggtagttttt aaaatcatct
     4981 gcacttcgta ttcttgctcc agcttacctt cccagtaata gagagaggta gcgccgggga
     5041 tcaaggtcgc gcaggccgcc agtttttccg ccagcacttt ggcggctaaa tcctgggctg
     5101 tcgcttcatc cggtgcggta catagcacca cgacagacgc ggtattcgaa cttttttcat
     5161 caagcataaa cacctcgcaa gaacagatgg aaccgcaaga gagaggtcac tatacaacgg
     5221 acgggggaag gatgttagtc ggcgggaaag aaagcggggc gtgaacgccc cgcgattggt
     5281 cattacagca tgaagctacc cagcacgaag ccgaagcaaa cggccagggc aacacccaga
     5341 gtacccggga tgaagaacgg atggttgaag acgaatttac cgatacgggt agtacccgtg
     5401 tcatccatct gtaccgcagc aaccagcgtc gggtaggtcg gcagaatgaa cagaccagac
     5461 accgcagcga aagaagcaac agcggtcagc ggtgaaacgt tcagtgccag agccatcggc
     5521 atcagtgctt ttgcggttgc agcctgagag tacagcagag cagaagcaaa gaagaagatg
     5581 acggccagca gccacggatg accctgaatc acttcaccag cggtatcttt gatccagtcg
     5641 atgttgttgg aaacgaaagt atcgcccagc cacgcaacac ccaggataca aatacaggcg
     5701 ctcatacctg ctttgaaggt gctggagttg aggatgttgt cggtatcgac tttacagata
     5761 acggtggtca gagttgcaac gctgagcatg atgatcagga ttgcgttggt ggtgttcatc
     5821 agcggttttt caaccagacc catgcttggg ctgttgatga ttgcatagat aaccacgcca
     5881 actacgccca gcaggaacag ccagacggac gtttttgcac cggatttgat ttcaatctgc
     5941 ttttcaccgc gcagttcaac caggccctct tccagacgct tgcgataaat cggatcgtca
     6001 gagagtttgg agttgaacag catagtgacc agaaaggaca tcaccagaac cgccagcagg
     6061 gtggacggga tgaccacgga gagcagatgg aggtagctga tgccatgacc ttccatcacg
     6121 gaagacatgt aaaccactgc cgctgagatt ggcgatgcgg tgatcgcaat ctgcgcggat
     6181 accactgcag tagacagcgg acggcaaggt ttgacgcctt gttccttcgc aacttcagcg
     6241 ataactggca gtgtcgccag agatatgttg ccagtaccag caaagatagt caggaaatag
     6301 gtcacgatcg gtgcgaggat cgtgatgtat ttcgggttac ggcgcagcag cttttctgtc
     6361 tgatgaacca gatagtccag accgccagca acctgcatgg cagaaatagc ggcgataacc
     6421 gccatgataa tggaaatgac atcgaacggg atgttaccgg gtttaacgcc aatagcggca
     6481 agaaccagca cccccaatcc gcctgcaaaa ccaataccta ttccccccaa tctggcgccc
     6541 aagaagatcg ccagcaaaac tatgatgagt tctacaacta gcatattagc cttccttgtt
     6601 ttttaacaag ttgatattag gttgttattt ttaagttact gctcacaaga aaaaaggcac
     6661 gtcatctgac gtgccttctt tatttgtact accctgtacg attactgttc gctttcatca
     6721 gtatagcgtt ttgctttgta agccgggtgc atcagattct gtacggagaa aatatcgtca
     6781 agttccgctt cagtcaacag accgcgttcc agaacgactt cacgtacact cttaccggtt
     6841 tcggcacaga ttttacccac gatgtcaccg ttgtggtgac cgatgaacgg gttcaggtaa
     6901 gtaacgatac cgatagagtt gtaaacgtaa ccttcgcaca cttctttgtt agcagtgatg
     6961 ccgttaatgc atttttccag caggttgtag caagcgttgg tcagaatgtg aacggattcg
     7021 aacatggcct ggccaatgac cggctccata acgttcaact gcagctgacc tgcttctgct
     7081 gccatggtaa cagtggtgtc gttaccgatg actttgaagc atacctggtt aaccacttcc
     7141 ggaacaaccg ggtttacttt agctggcatg atggaagagc ccgcctgcag ttccggcagg
     7201 ttgatctcgt tcaggccggc acgtggacca gaagagagca agcgcaggtc gttacagatt
     7261 ttggacatct tcacagccag gcgtttcagc gcgccgtgaa ccataacata agcgccgcag
     7321 tcagaggtcg cttcgatcag gtcttcagcc ggtacgcatg ggaagccagt gacttcagcc
     7381 agttttttca ctgccagcgg agagtactct ttcggcgtgt tcagaccagt accgattgct
     7441 gttgcgccaa ggttaacttc cagcagcagt tcagcggtac gttggatatt tttcacttct
     7501 tctttcagca ggatgctgaa agcgcggaat tcctgaccga gggtcatcgg tactgcgtcc
     7561 tgcagctggg tacgacccat tttcaggatg tcctggaatt cgacagcttt acgttcaaag
     7621 ccttcacgca gttggttaat cgcatctacc agcttaatca gggaagagta aactgcgata
     7681 cggaaaccgg tcgggtaggc gtcgttagtg gactgacatt tgttaacatg gtcgttcggg
     7741 ttcaggtact gatattcacc tttctggtga cccatcagtt ccagaccgat attggccagc
     7801 acttcgttgg tgttcatgtt tacggaagta cctgcgccgc cctggtagac gtctaccggg
     7861 aactgatcca tgcattttcc gttgttcagg acttcatcac atgcggcaat gatggcattc
     7921 gctacacttt taggaatggt ttgcagctct ttgtttgcca tagctgcggc ttttttaacc
     7981 attaccatac cgcgaacaaa ttcaggaata tcactgattt tgttgttgct gatatagaag
     8041 ttttcaatcg ctctcagagt gtgaacacca tagtaggcat cagctggaac ttccctggta
     8101 cccaacagat cttcttcgat acgaatgttg tttgacatgt gaaccttctt tttcaagctg
     8161 ccaatgattt gctttaaaca cacagaatat atgtggtttc gaatgttttt cgaccgacga
     8221 ttatcccctg catcgaccga atacccgaga tcatatgctg cttgaggatt tctaccgtaa
     8281 tctggatcac tttaagtgtc ggtttttacc ccttaattat taatttgtga aatagatcac
     8341 cgctttggga ttactaccaa aaatagttgc gcaaacatct tgaaattttg ctaatgacca
     8401 caatataagc tgaacgcgat tcgcaaccca ttcaggtagc cgggttttac cggctgctat
     8461 tacaggagaa acctttgcgc tggttacctt ttattgccat tttcctttat gtctatattg
     8521 agatttcaat ctttattcag gttgcccatg tattgggggt attgctgacc ctcgtgctgg
     8581 ttatattcac gtcagttatc gggatgtcac tggtacgtaa ccagggcttt aagaatttcg
     8641 tgctgatgca gcaaaaaatg gcggcgggtg aaaacccagc ggcggagatg attaaaagtg
     8701 tttcgctgat cattgccggt ttgctgcttt tattaccggg ctttttcacc gacttcctcg
     8761 gtcttctgct tttattaccg ccggtgcaaa agcatctgac agtgaagttg atgccgcatt
     8821 tgcgcttttc tcgtatgcca ggcggcggtt ttagcgccgg gaccggtggc ggtaatactt
     8881 ttgatggtga gtaccagcga aaggatgatg agcgcgaccg ccttgatcat aaagacgatc
     8941 gccaggatta atgtcgaaac gccggattat gtggttatgc cattttccgg cgtttttcgt
     9001 tttggcagca acagccataa ccccgccagc atgatcagcg catagagact tttccagccg
     9061 accattgcca gtaacagaac gcataacagc ccgccaacca ccgccagtag tcgataacgt
     9121 ccttgcaata atttacagcc tgccagcatg cataacagat aaatcataat aaagatgcca
     9181 ttggcataaa taataagagc gtccagattg atctctaaag catgaatcac caacgtgctc
     9241 accacacagc agccgagcac cgcattgagg gcattattcg ggatatggcg agaagagagg
     9301 cgtgccaggt agtggtcagg attatgttgc gcctgcgacc agaccaggcg ggcgaagctc
     9361 tgtatataaa tgttgagact ggcaaagcag gccagatagc caatcacgca ggcaatccat
     9421 aacgctccta caccgaacaa ctgcactaca atttttggaa gcgatgctgc cgccgccatt
     9481 ttttcaccat aggcgtcgaa gtgtaagacg actaccgtac agccccagta gactaatcct
     9541 gccagcagca gaccaatcat caatgcgcga ggaaaatcac gctctggatt tttaaattcc
     9601 gaggcaagat gggcaaatgc ttccagaccg acaaaacacc agaacatcac tgataacgca
     9661 gcaaataacc cggtaagttc gatattacca ggtgccggga aggggatatt cgcaggtttg
     9721 atatcgcccg cccaccagat agcgacaatc agcgcgacga taagtccggc aataacggtt
     9781 tgtagattag cactggaact ggcaccgcga gtaccgatat accacaccag cgccagcgta
     9841 ccgagttctg ccaacaacag ttgccagcta tgccagccaa acattgcctg gccgaacccg
     9901 gcggcaattt gtagcgcggc aggcaaaccc acgggaatga ccgataaaaa caaccagccg
     9961 gtgactcgct caagtcgcga accaaacgcc ataccgacga agtgcgcgac accgcctgcg
    10021 ctgggatagt ggcgacccag aatcgcaaac acaatcgcaa tcgggaacac taagataatc
    10081 aaaacgggcc acgcccacag gctgttattg cccgctacca gcgcagctaa cgcaggaacg
    10141 gcaaacacgc cagtgcctaa taatgacgtc gatagcaggc caatgccttg ggccagcccc
    10201 agttcttgtt tgagtccact catgggttga tatccgattg cgcccaaatt ttgggcaact
    10261 gcgtagattt tcgatggtag cacaatcaga ttcgcttatg acggcgatga agaaattgcg
    10321 atgaaatgtg aggtgaatca gggttttcac ccgattttgt gctgatcaga attttttttc
    10381 tttttccccc ttgaaggggc gaatcctcat ccccatttct ctggtcacca gccgggaaac
    10441 cacgtaagct ccggcgtcac ccataacaga tacggacttt ctcaaaggag agttatcaat
    10501 gaatattcgt ccattgcatg atcgcgtgat cgtcaagcgt aaagaagttg aaactaaatc
    10561 tgctggcggc atcgttctga ccggctctgc agcggctaaa tccacccgcg gcgaagtgct
    10621 ggctgtcggc aatggccgta tccttgaaaa tggcgaagtg aagccgctgg atgtgaaagt
    10681 tggcgacatc gttattttca acgatggcta cggtgtgaaa tctgagaaga tcgacaatga
    10741 agaagtgttg atcatgtccg aaagcgacat tctggcaatt gttgaagcgt aatcctcgca
    10801 cgacactgaa catacgaatt taaggaataa agataatggc agctaaagac gtaaaattcg
    10861 gtaacgacgc tcgtgtgaaa atgctgcgcg gcgtaaacgt actggcagat gcagtgaaag
    10921 ttaccctcgg tccgaaaggc cgtaacgtag ttctggataa atctttcggt gcaccgacca
    10981 tcaccaaaga tggtgtttcc gttgctcgtg aaatcgaact ggaagacaag ttcgaaaata
    11041 tgggtgcgca gatggtgaaa gaagttgcct ctaaagcgaa cgacgctgca ggcgacggta
    11101 ccaccactgc aactgtactg gctcaggcta tcatcactga gggtctgaaa gctgttgctg
    11161 cgggcatgaa cccgatggac ctgaaacgtg gtatcgacaa agccgttacc gctgcagttg
    11221 aagaactgaa agcgctgtcc gtaccgtgct ctgactctaa agcgattgct caggttggta
    11281 ccatctccgc taactccgac gaaaccgtag gtaaactgat cgctgaagcg atggacaaag
    11341 tcggtaaaga aggcgttatc accgttgaag acggtaccgg tctgcaggac gaactggacg
    11401 tggttgaagg tatgcagttc gaccgtggct acctgtctcc ttacttcatc aacaagccgg
    11461 aaactggcgc agtagaactg gaaagcccgt tcatcctgct ggctgacaag aaaatctcta
    11521 acatccgcga aatgctgccg gttctggaag ctgttgccaa agcaggcaaa ccgctgctga
    11581 tcatcgctga agatgttgaa ggcgaagcgc tggcaactct ggttgttaac accatgcgtg
    11641 gcatcgtgaa agttgctgcg gttaaagctc cgggcttcgg cgatcgtcgt aaagctatgc
    11701 tgcaggatat cgcaaccctg actggcggta ccgtaatctc tgaagagatc ggtatggagc
    11761 tggaaaaagc aaccctggaa gacctgggtc aggctaaacg tgttgtgatc aacaaagaca
    11821 ccaccactat catcgatggc gtgggtgaag aagctgcaat ccagggccgt gttgctcaga
    11881 tccgtcagca gattgaagaa gcaacttctg actacgaccg tgaaaaactg caggaacgcg
    11941 tagcgaaact ggcaggcggc gttgcagtta tcaaagtagg tgctgctacc gaagttgaaa
    12001 tgaaagagaa aaaagcacgc gttgaagacg ccctgcacgc gacccgtgct gcggtagaag
    12061 aaggcgtggt tgctggtggt ggtgttgcgc tgatccgcgt agcgtctaaa ctggctgacc
    12121 tgcgtggtca gaacgaagac cagaacgtgg gtatcaaagt tgcactgcgt gcaatggaag
    12181 ctccgctgcg tcagatcgtc ctgaactgcg gcgaagaacc gtctgttgtt gctaacaccg
    12241 ttaaaggcgg cgacggcaac tacggttaca acgcagcaac cgaagaatac ggcaacatga
    12301 tcgacatggg tatcctggac ccaaccaaag taacccgttc tgctctgcag tacgcggctt
    12361 ctgtggctgg cctgatgatc accaccgagt gcatggttac cgacctgccg aaaaatgatg
    12421 cagctgactt aggcgctgct ggcggtatgg gcggcatggg tggcatgggc ggcatgatgt
    12481 aattgccctg cacctcgcag aaataaacaa acccccgggc agaaatgtct gggggttttt
    12541 cttttggtca tctttctagt ataagattca gacacggacg acgcgagtgg cgtccagctc
    12601 attgattatg gggaataaca tgcacgtaaa atacttagca gggattgtcg gtgccgcgct
    12661 actgatggcg ggttgtagct ccagcaacga attgagtgct gccggtcaga gtgtacgcat
    12721 tgtggacgag cagccaggcg cagagtgcca gctgattggt actgcgacag gtaagcaaag
    12781 caactggctt tccgggcaac acggagaaga gggcggttct atgcgcggcg cagcaaacga
    12841 tctgcgcaac caggcggctg caatgggcgg taacgtgatt tatggcatca gcagcccgtc
    12901 gcagggaatg ttgtccagtt ttgtcccgac ggatagccag attatcggtc aggtttataa
    12961 gtgcccgaac tgatgtgatc cagaggctgg gtgacaaacg tatca
//
LOCUS       DAMZII010000134        13001 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZII010000134 DAMZII010000000
VERSION     DAMZII010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.134"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            213..548
                     /locus_tag="POA77_003823"
     CDS             213..548
                     /locus_tag="POA77_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDI6367186.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            708..1151
                     /gene="yjdN"
                     /locus_tag="POA77_003824"
     CDS             708..1151
                     /gene="yjdN"
                     /locus_tag="POA77_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDI6367187.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1284..2072
                     /gene="phnC"
                     /locus_tag="POA77_003825"
     CDS             1284..2072
                     /gene="phnC"
                     /locus_tag="POA77_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI6367188.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2097..3113
                     /gene="phnD"
                     /locus_tag="POA77_003826"
     CDS             2097..3113
                     /gene="phnD"
                     /locus_tag="POA77_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6367189.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3238..4017
                     /gene="phnE"
                     /locus_tag="POA77_003827"
     CDS             3238..4017
                     /gene="phnE"
                     /locus_tag="POA77_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6367190.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4038..4763
                     /gene="phnF"
                     /locus_tag="POA77_003828"
     CDS             4038..4763
                     /gene="phnF"
                     /locus_tag="POA77_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDI6367191.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4764..5216
                     /gene="phnG"
                     /locus_tag="POA77_003829"
     CDS             4764..5216
                     /gene="phnG"
                     /locus_tag="POA77_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDI6367192.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5213..5797
                     /gene="phnH"
                     /locus_tag="POA77_003830"
     CDS             5213..5797
                     /gene="phnH"
                     /locus_tag="POA77_003830"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDI6367193.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5797..6861
                     /gene="phnI"
                     /locus_tag="POA77_003831"
     CDS             5797..6861
                     /gene="phnI"
                     /locus_tag="POA77_003831"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDI6367194.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6854..7699
                     /gene="phnJ"
                     /locus_tag="POA77_003832"
     CDS             6854..7699
                     /gene="phnJ"
                     /locus_tag="POA77_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDI6367195.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7696..8454
                     /gene="phnK"
                     /locus_tag="POA77_003833"
     CDS             7696..8454
                     /gene="phnK"
                     /locus_tag="POA77_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDI6367196.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8565..9245
                     /gene="phnL"
                     /locus_tag="POA77_003834"
     CDS             8565..9245
                     /gene="phnL"
                     /locus_tag="POA77_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDI6367197.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9242..10378
                     /gene="phnM"
                     /locus_tag="POA77_003835"
     CDS             9242..10378
                     /gene="phnM"
                     /locus_tag="POA77_003835"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDI6367198.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVTDDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10378..10935
                     /gene="phnN"
                     /locus_tag="POA77_003836"
     CDS             10378..10935
                     /gene="phnN"
                     /locus_tag="POA77_003836"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDI6367199.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10922..11356
                     /gene="phnO"
                     /locus_tag="POA77_003837"
     CDS             10922..11356
                     /gene="phnO"
                     /locus_tag="POA77_003837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDI6367200.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11358..12116
                     /gene="phnP"
                     /locus_tag="POA77_003838"
     CDS             11358..12116
                     /gene="phnP"
                     /locus_tag="POA77_003838"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDI6367201.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12263..12592)
                     /gene="yjdP"
                     /locus_tag="POA77_003839"
     CDS             complement(12263..12592)
                     /gene="yjdP"
                     /locus_tag="POA77_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDI6367202.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDLERRQLEDEERR
                     MEDEYGR"
     gene            complement(12661..12978)
                     /locus_tag="POA77_003840"
     CDS             complement(12661..12978)
                     /locus_tag="POA77_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDI6367203.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2840 a         3916 c         3663 g         2582 t
ORIGIN      
        1 aaagaacaag tagagcatta accttatctt aaataataaa tggaggcaaa aagattattt
       61 tctttttgcg tttcctttca aatgaaaacg atcgtcgtct aaaatcagca gtacccccga
      121 caaactcagg gattttgtgt ataattgcgg cctttttcgg caatctgccg ttttttggcg
      181 cttttgccct gctgactttt gaggaaatcc acatgtcatt accacactgc ccaaaatgca
      241 actccgaata cacttacgaa gataacggca tgtacatctg cccggaatgt gcctacgaat
      301 ggaacgacgc agaacctgca caggaaagcg acgagctgat cgttaaagat gctaacggca
      361 atctgctggc tgacggcgac agcgttacca tcattaaaga tctgaaggtg aaaggtagct
      421 cttcgatgct gaaaattggc accaaagtga aaaacatccg cctggttgaa ggcgaccata
      481 acatcgattg caaaatcgac ggttttggtc cgatgaaact gaaatctgag tttgtgaaaa
      541 agaactgatt gtattgtgat cggtaagccg gataaggcgc tcgcgccgca tccggcaacg
      601 atgccgactg cctgatgcga cgctgaccgc gtcttatcag gcttccctct catctgtata
      661 aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg ccgttaagtc
      721 cctacctctc ttttgccggt aactgtgccg acgcaattgc ttattatcaa cgtacgttgg
      781 gcgctgaact gctctataaa atcagcttcg gcgaaatgcc aaaatcagcg caggacagcg
      841 ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgctcat gccaacgtgc
      901 gcattgccgg aagcgacatc atgatgagcg atgccatgcc gtcaggaaaa gccagctact
      961 ccggctttac gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc tggtttgaca
     1021 atcttgccgc taacggaaaa atcgaaatgg cctggcagga aactttctgg gcgcatggct
     1081 ttggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcgtc aaacaacaac
     1141 caacgcaata atccgccggg aggcccgccc tcccgcactg tcatcgaatt cccgttaact
     1201 cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc
     1261 cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa
     1321 ccttcaatca gcatcaggcg ctgcatgcgg ttgatctgaa cattcatcac ggtgaaatgg
     1381 tggctctgct tgggccgtcg ggttccggca aatccaccct tttacgtcac ttaagcggtt
     1441 tgattaccgg cgataaatcc gccggcagcc atatcgagct gctgggccgc acagtccagc
     1501 gcgaaggccg tctggcgcgc gatatccgca aaagccgcgc caacaccggc tacatcttcc
     1561 aacaattcaa cctggtgaac cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg
     1621 gcagcacgcc gttctggcgc acctgtttta gctggttcac cggcgagcag aaacagcgcg
     1681 cgttacaggc gctgacccgc gttggcatgg tgcattttgc ccatcagcgc gtttccaccc
     1741 tctccggcgg acagcagcag cgtgtggcga ttgcccgcgc gctgatgcag caggcgaagg
     1801 tgattctggc cgatgaaccc atcgcctcgc tggacccgga atccgcccgc atcgtgatgg
     1861 acaccctgcg cgacatcaat cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg
     1921 tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg cacgttttct
     1981 acgacggcag cagccaacag tttgataacg aacgttttga ccatctctac cgcagcatta
     2041 atcgcatcga agagaacgcg aaagctgcct gacatcccca tcattgagga aaacgaatga
     2101 acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa
     2161 gcccggcaca cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat
     2221 cacagcaaaa cctgaaaccg caatggacgc cattcttaca ggatatggag aagaagctgg
     2281 gcgtgaaggt gaacgccttc tttgccccag actacgcagg cattatccag ggaatgcgct
     2341 tcaataaagt ggatatcgcc tggtacggca acctgtcggc aatggaagcg gtggatcgcg
     2401 ccaacggcca ggtctttgcc cagacggtcg cggcggatgg atcgccgggt tactggagcg
     2461 tgttgatcgt caacaaagac agcccgatca acaacctgaa cgatctgctg gcgaagcgga
     2521 aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggtt
     2581 actacgtctt tgccaaaaac aatatctccg ccagcgactt taagcgcacc gtcaacgctg
     2641 ggcatgaaac taacgcgctg gccgtcgcca acaagcaggt ggatgttgcc accaacaaca
     2701 ccgaaaacct cgacaagctg aaaacctccg cgccagagaa gctgaaagaa ctgaaggtga
     2761 tctggaagtc gccgctgatc ccaggcgatc cgatcgtctg gcgcaagaat ctctccgaaa
     2821 ccaccaaaga caagatctac gacttcttta tgaactacgg aaaaacgccg gaagagaaag
     2881 cggtgctgga acgcctgggc tgggccccgt tccgcgcctc cagcgacctg caactggtgc
     2941 cgattcgcca gctcgcactg tttaaagaga tgcagggcgt aaaaagcaat aaaggactga
     3001 atgagcagga caagctggcg aaaaccaccg agattcaggc gcagctggat gacctggacc
     3061 gcctgaacaa cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taatgtgctc
     3121 gatcggtccc ctctcccttg agggagaggt ttagggtgag ggggaatgcg cacgctgatg
     3181 ccctcacccc ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg
     3241 caaaccatca ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccgtt
     3301 gttctcgccg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
     3361 aaagacggcg gcaacatggc aaccttcgct gccgacttct tcccgcccga tttcagccag
     3421 tggcaggatt acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcacggcg
     3481 ctggcggtgg ttctctccat ccccttcggt ctgatgagcg ccgaaaacct ggtgccgtgg
     3541 tgggtttacc agcccgttcg ccgcctgatg gatgcctgcc gcgccattaa cgaaatggtc
     3601 ttcgccatgc tgttcgtggt cgccgtcggt ctcggcccgt tcgctggcgt gctggcgcta
     3661 tttatccaca ccaccggcgt gctctccaag ctgctttccg aggcggtgga agcgattgaa
     3721 cccggcccgg tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctctac
     3781 ggcgtgctgc cacaggtgat gccgctgctg atctcctact ccctctatcg cttcgaatcc
     3841 aacgtccgct cggcgaccgt cgtcggcatg gtcggcgcgg gcgggatcgg cgtcacgctg
     3901 tgggaagcga ttcgcggttt ccagttccag caaacctgcg ccctgatggt gctgattatc
     3961 gtcacggtca gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa
     4021 gcgaggcatt gatatctatg cacttgtcta cacatccgac cagctatcca acacgctatc
     4081 aggagatagc cgcaaaactt gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc
     4141 ttcccgccga gcagcaactg gcggcgcgct ttgaggtgaa ccgccacacc ctgcgccgcg
     4201 ccatcgacca actggtggaa aaaggctggg tacagcgccg tcagggcgtc ggcgtgctgg
     4261 tgctgatgcg cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc
     4321 tggaccaggg tagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg
     4381 gtcacgtcgc tgacgcgctg gggattaccg agggcgagaa cgttatccat ttgcgcaccc
     4441 tgcgtcgcgt caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc
     4501 tttggccgac gctgcaacgc ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa
     4561 ccggaattgc gctgcgccgc agccagacgc ggatcagcgc ccgtcgcgcc caggccaaag
     4621 agtgccagcg tcttgaaatt ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc
     4681 accgtgacgg tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga
     4741 ttgaattcac tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt
     4801 ccgtactggc gcacagccaa ccggctgaac tggcagcccg cctgaaagca ctgaacatca
     4861 ccgccgacta tgaggttatc cgcgccgccg aaaccggcct ggtgcagatt caggcgcgga
     4921 tgggcggcac cggcgaacgc ttttttgccg gcgacgccac gctcacccgc gccgccgtac
     4981 gcctgactga cggcacgctc ggctacagct gggtgctggg gcgcgacaaa cagcacgccg
     5041 aacgctgcgc gctgattgac gcgctgatgc agcaatctcg ctattttcaa aacttatcag
     5101 aaacccttat tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg
     5161 aagtgaacgc cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct
     5221 ggaaaccgct tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa
     5281 ggccatgagc gagccgggcg tgattgtcgc cctgcatcag ctcaagcgcg gctggcagcc
     5341 gctgaatatc gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg
     5401 gcttgccgcg ccattgagta acgatatcgt cagccagagc ctgcgttttc ataccaacgc
     5461 gccgctggtc agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag
     5521 cgaacagctt aacgcccttt ccaccggcac cgccgttgcg ccggaagcgg gtgcaacgct
     5581 gattttacag gtcgccagcc tgagcggcgg gcgcatgtta cgtctcaccg gcgcgggcat
     5641 tgccgaagaa cgaatgatcg ctccgcagct gccggagtgc attctgcacg aactcaccga
     5701 gcgcccgcac ccgttcccgc tcggcatcga cctgatcctg acctgcggcg agcgcctgct
     5761 ggctattccg cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc
     5821 gagaaggcga tcgacgccgc tcacgccctg caagagagcc gacgccgggg cgataccgat
     5881 ttgcctgaac tgagcgtcgc ccagattgaa cagcagctta atcttgcggt ggatcgcgtg
     5941 atgaccgaag gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc
     6001 ggcgataacg tcgaagcaat tttcctgctg cgcgcctacc gcaccacgct ggcgaagctc
     6061 gcggtaagcg agccgctcga caccacagag atgcgtctcg aacggcgtat ttcggcggtt
     6121 tataaagaca ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg
     6181 ctcgatttca ccctgctggc aaacggtgaa gcgccgacgc tgaccaccgc cgacagcgaa
     6241 caacagccgt cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa
     6301 gaggatagcg gcgcacagcc ggatgatatc acccgcacgc cgccggttta cccctgctca
     6361 cgttcttccc gtttgcagca gttgatgcgc ggcgacgaag gctatttgct ggcgctggcc
     6421 tactccaccc aacgcggtta cgggcgcaat cacccgttcg cgggcgagat ccgcagtggt
     6481 tacatcgatg tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaacta
     6541 ctgatgaccg agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac
     6601 ttcacgcgcg gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg
     6661 ctggtcgatc gtgcgttgca ggctccggaa tacggcgagc acgcgacagg cccggcgcag
     6721 gatgaagagt ttgtgctggc acatgccgac aacgtcgaag ccgcaggctt tgtctcgcac
     6781 ctcaaactcc cccactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa
     6841 caggagaaga accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac
     6901 caaacgcatg atccgccgcg ctatcttaaa agcggtggcg atccccggtt atcaggtgcc
     6961 gtttggcggg cgcgagatgc cgatgccata cggctgggga accggcggca tacagctcac
     7021 cgccagcgtg attggcgaaa gcgacgtgct gaaggtgatt gaccagggtg cggatgacac
     7081 caccaacgcc gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga
     7141 acgtacggac gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac
     7201 cgaagatcag atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc
     7261 gcgcgaaacg gaaacccgca ccatgcacgc gctggaagag tacggcgtga tgcaggtgaa
     7321 actgtatgaa gatatcgccc gcttcggtca tatcgccact acctacgcct atccggtgaa
     7381 ggtgaacggg cgctacgtaa tggacccgtc gccgatcccg aaattcgata acccaaaaat
     7441 ggacatgatg cccgccctgc aactgttcgg cgcggggcgc gagaagcgca tctatgcggt
     7501 gccgccgttt acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca
     7561 gtgggatgag ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct
     7621 ggatgacgcc ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag
     7681 cgaggcaaaa aaccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac
     7741 gcgccgggca aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc
     7801 attgtcgggg agtccggctc cgggaagacc acgctgctga aatcaatctc cgcgcgcctg
     7861 acgccgcagc agggggagat tcactatgag agccgttcgc tgtacgcaat gagcgaggcc
     7921 gaccgccgtc gcctgctgcg caccgaatgg ggcgtagtgc atcagcatcc actcgacggc
     7981 ctgcgccgtc aggtctcggc aggcggcaat atcggcgagc ggctgatggc gaccggggca
     8041 cgtcattacg gcgatattcg tgccaccgcg cagaagtggc tggaagaggt ggaaattccc
     8101 gccaaccgga tcgacgacct gccgaccacc ttttccggcg gcatgcagca acgtttgcag
     8161 atcgcccgca acctggtgac gcatccgaag ctggtgttta tggacgagcc gaccggcgga
     8221 ctggatgtgt cggtacaggc acgcctgctc gacctgctgc gcggcctggt ggtggagctg
     8281 aacctcgcgg tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt
     8341 ttgctggtga tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac
     8401 gacccgcacc atccgtatac acaactgctg gtgtcatcgg ttttgcagaa ttgagccggt
     8461 gccggatgcg gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa
     8521 gacgcgtcag cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa
     8581 acgtcagtaa aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcttacaac
     8641 gtgcctcgct caccgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg
     8701 gcaaatcaac cttgctgcgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc
     8761 agatcaaaca cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg
     8821 agatccgcaa aaccaccgtc ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt
     8881 cagcactgga agtggtgatg cagccgctgc tcgataccgg cgttccgcgt gaagcctgcg
     8941 ccgctaaagc cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcacctgg
     9001 cgccatcaac cttttccggt ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg
     9061 tcgactaccc cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg
     9121 ccgccgtagt cgagctgatt cgcgaagcca aaacccgtgg cgcggccatc gtcggcatct
     9181 tccatgacga agccgtacgt aatgacgtcg ccgaccgctt gcacccaatg ggagcctctg
     9241 catgattatc aataacgtca aactggtgct ggaaaacgaa gtggtacacg gttcgctgga
     9301 ggtgcaggat ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cagaagcgat
     9361 ggacggcgaa ggtggctggc tactacccgg cctgattgag ctgcataccg acaacctcga
     9421 taaattcttc accccgcgcc cgaaagtcga ctggcccgcc cattcggcga tgagcagcca
     9481 cgacgcgctg atggtggcaa gcggcatcac caccgtgctg gacgcggtgg cgattggcga
     9541 cgtgcgcgac ggcggcgatc ggctggagaa tctggagaag atgatcaaca ccatcgaaga
     9601 gacgcagaaa cgcggcgtca accgcgccga gcaccgtctg cacctgcgct gcgagctgcc
     9661 gcatcacacc acactgccgc tgtttgaaaa actggttcag cgcgaaccgg tgacgctggt
     9721 gtcgctgatg gaccactcac cgggccagcg ccagttcgct aaccgcgaga agtatcgcga
     9781 atattatcag ggcaaatact ccctcaccga tgcgcagatg cagcagtacg aagaagagca
     9841 attggcgctc gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg
     9901 cgcgcgacaa attgcgctcg ccagccacga tgacgccacg cacgcccacg ttgccgaatc
     9961 ccaccaactt ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc
    10021 gcgtaagcat ggcatgaacg tgctgatggg tgcgccaaat atcgtgcgtg gcggctcgca
    10081 ctccggcaat gtggcggcca gtgaactggc gcagcttggt ctgctggata tcctctcttc
    10141 cgactactac cccgccagcc tgctggatgc ggcgttccgc gtcaccgatg acgagagcaa
    10201 ccgctttacg ctaccgcagg cggtgaggct ggtgaccaaa aatccggcgc aggcgctgaa
    10261 tcttcaggat cgcggggtga ttggcgaggg taaacgcgct gacctggtgc tggcgcatcg
    10321 ccagggcaat cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg
    10381 atgggaaaac tgatttggtt aatggggccg tccggctccg ggaaagacag cctgctggcg
    10441 gaactccgcc tgcgggaaca aacgcagtta ctggtggcgc atcgctacat cacgcgcgac
    10501 gccagcgccg gaagtgaaaa ccatatcgcc ctgagtgagc gggagttttt tacccgcgcg
    10561 gggcaaaacc tgctggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcatc
    10621 gagattgacc tctggctgca cgctggattc gacgtagtcg tcaacggttc acgcgcgcat
    10681 ttgccgcagg cacgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg
    10741 ccggagatcc tgcgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac
    10801 gcccgcctgg cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac
    10861 ggcagcctgc gccagtcggt cgacacgctg ctgacgctga tccatcagaa ggagaaacac
    10921 catgcctgct tgtgagcttc gcccggccac gcagtacgat accgacgcgg tttacgcgct
    10981 gatttgtgag ctaaaacagg cggagtttga ccaccatgcg tttcgcgtgg gctttaacgc
    11041 caacctgcgc gacccaaaca tgcgctacca tctggcgctg ctggatggcg aagttgtcgg
    11101 catgatcggc ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca
    11161 ggaactggtg gtgatgccgc aggcgcgcgg tctgaacgtc ggcagtaaat tactggcatg
    11221 ggcagaagaa gaagcccgcc aggccggggc cgaaatgacc gagctttcca ccaacgtgaa
    11281 gcgccacgac gcacaccgtt tttacctgcg tgaaggctac gagcagagcc acttccgctt
    11341 caccaaggcg ctgtaacatg agcctgaccc tcacgctcac cggcactggc ggcgcacagg
    11401 gcgttccggc atggggctgc gagtgcgcgg cctgcgccag agcgcggcgc tcgccgcagt
    11461 atcgccgcca gccatgcagc ggcgtagtga agtttaacga cgcgatcacc ctgatcgacg
    11521 ccgggcggca cgatctcacc gatcgctggt cgcctggatc gttccagcag tttttgctga
    11581 cgcactacca tatggatcac gtccaagggc tatttccgct acgctggggc gtgggcgatg
    11641 tgatcccggt ctacggcccg ccggatgaac agggctgcga cgatctgttt aaacatccgg
    11701 gcctgcttga tttcagccac acagtggaac cgtttgtggt gtttgatttg caggggttgc
    11761 aggtcacgcc gctgccgctt aaccactcaa aactgacctt cggttatctg ctggaaacgg
    11821 cacatagccg ggtggcgtgg ctgtctgaca ccgctggcct gccggaaaaa acgctgaaat
    11881 ttttactcaa taatcatccg caggtgatgg tgattgattg cagtcacccg ccgcgcgcgg
    11941 atgcaccgcg taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct
    12001 cgccacaggt gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaacg
    12061 cactaccgtc agggtttgag gtggggtttg atgggatgga gattggggtg gcgtgatgag
    12121 agggaatgtg cgcgctggcc ccctcaccct aaccctctcc ccagaggggc gaggggaccg
    12181 attgtgctca atattgaata ttgcgctcgt tttctccctc tccccattgg ggtgaggggc
    12241 gatgcctgct ccatacccaa cctcatcgcc catactcatc ttccattctc cgctcttcat
    12301 cctccagttg ccgacgctcc agatcaagct ggcgctggcg atcgtccagc tgcctgcggc
    12361 gatcttcaaa ctggcggcgg cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac
    12421 gctgccagcc gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt
    12481 cattaatcgc ctgctgaata ttaccaatag tatcgtcgat aatatcagcc tgggccggaa
    12541 cggtggacag cgtgagcagg gtgaataaaa gaaatagcgg aaagcgtttc attagccaac
    12601 ctcaaaaaga aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc
    12661 tcaatttctc cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc
    12721 ttcgtactgt gcgcccagcc acgcatccac aatcattttt gccagttcga ggccaaccac
    12781 tcgtgaacca aaagccagtg caacttcact ggcataatgc ggataatcag tgcgctctga
    12841 cgacctggtg tctttatcaa tcacctcact cattgaccat gaataaaagg ctggcacagc
    12901 aacgcagaat cctgcggtgc cagcgacatg accgcccatt gctccggtac gttttttaaa
    12961 aagaaacacc ggttgcattg ataaattgtg ctcagtattt g
//
LOCUS       DAMZII010000135        12927 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZII010000135 DAMZII010000000
VERSION     DAMZII010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.135"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(48..2081)
                     /gene="betT"
                     /locus_tag="POA77_003841"
     CDS             complement(48..2081)
                     /gene="betT"
                     /locus_tag="POA77_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDI6367204.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2210..2797
                     /gene="betI"
                     /locus_tag="POA77_003842"
     CDS             2210..2797
                     /gene="betI"
                     /locus_tag="POA77_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDI6367205.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2811..4283
                     /gene="betB"
                     /locus_tag="POA77_003843"
     CDS             2811..4283
                     /gene="betB"
                     /locus_tag="POA77_003843"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI6367206.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIVKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4297..5967
                     /gene="betA"
                     /locus_tag="POA77_003844"
     CDS             4297..5967
                     /gene="betA"
                     /locus_tag="POA77_003844"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI6367207.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6180..6845
                     /locus_tag="POA77_003845"
     CDS             6180..6845
                     /locus_tag="POA77_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367208.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(7091..7786)
                     /locus_tag="POA77_003846"
     CDS             complement(7091..7786)
                     /locus_tag="POA77_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDI6367209.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7779..9206)
                     /locus_tag="POA77_003847"
     CDS             complement(7779..9206)
                     /locus_tag="POA77_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDI6367210.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9217..9936)
                     /locus_tag="POA77_003848"
     CDS             complement(9217..9936)
                     /locus_tag="POA77_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDI6367211.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10463..11317)
                     /gene="rclR"
                     /locus_tag="POA77_003849"
     CDS             complement(10463..11317)
                     /gene="rclR"
                     /locus_tag="POA77_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDI6367212.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11543..12868
                     /locus_tag="POA77_003850"
     CDS             11543..12868
                     /locus_tag="POA77_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDI6367213.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTAENIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3343 a         3376 c         3180 g         3028 t
ORIGIN      
        1 gattcccgcg ccatatgcag acatggcgcg gttttatgca aaaacaatca cgcgtccggg
       61 aacatcacgc tatggcccgg cgcttcacga tggagatgaa taaagttaag atgccgctcg
      121 tactggtcaa gaatatcggt tatcacctgc tctttgctgt agtccatcag gtcgttgccc
      181 tggctgcctt ctaacaggaa ggtttccagc cggtagtagg tcgatttacc gctacgtgcg
      241 cggtaggtaa agcccggcac cgaatattgc tgcggccaaa tctgatagac aaagttttgt
      301 tcttcgccca tatgcaccaa caaatccaga tgccccaact gttgtccctc ttccggcggc
      361 aggcttttta gctccacgta cgcgccgcgc aaccgcagct cctgcgccac ttcttccatt
      421 gccgggtaac agaccgtctc catcatctgt ttagtgtaac gcgtgcccgg ataattcatc
      481 aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa gccccagcgg tcgcggcgcg
      541 gtatcgcggt tggcactttc acggcggtaa tcttctacct tcagagattt atacaacccc
      601 gccatcacga agaaaatcac aaagctgaac ggcagcccca tgattaccgt ggtgttttgc
      661 agcgcggata tcccgttggt catcagcatg ccgagcgtca gcagaccaat cgccaccgac
      721 cagaagacgc gcagccagcc gggggcgtcg ctgttgatat ctttaagctg cgaggtgaaa
      781 ttccccagca ccagcgcacc ggagtccgcc gaggtcacat aaaacagcag gccagtaatg
      841 gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg ccagcaggct gtagaagccg
      901 cgctccggat ggaccatcgc ttcctcggca aatgccgcgc cgccgtggat gatttcatac
      961 agcgcgctat tgccgaacac cgagagccat aacagcgtga aggtaaacgg aataatcaac
     1021 gtgcccagca cgaactggcg aatggtacgc ccacgcgaga tacgcgccag gaacaagccg
     1081 acaaacggcg accatgccac ccaccatgcc cagaagaaga gcgtccagtt attcatccac
     1141 tcaactggac ggtcgaaggc aaaactgttg agcgtcatgc ccataaagcg attcacatag
     1201 tcgccaacat tcagcaccag cgcattaagc aggaacgagg tgtcgcccat aaacaatacg
     1261 aacaggatca atcccagcgc cagcgcaacg ttaagttccg ataacacacg aatcccctta
     1321 tcgacaccgg aggtgacaga gatcgtggcg attatcaccg acaaggcgat cagtgccgct
     1381 tttgccgcca tcgaatcggg aatatcaaac agtacgctca agccatagtt aagctgcacc
     1441 acaccgatac cgagcgtagt ggcaataccg aagatggtgc cgatcaccgc tgcaatatcc
     1501 actgagtgcc ctatcggccc gttaatccgt ttaccgaaga tcgggtacag cgccgagcgg
     1561 atggtgagcg gcaaattata acgatagcta aagtatccga gcgccatgcc catcagcgca
     1621 tacatcgacc agccggttaa gccgtagtga aacagcgtcc agaccatcgc ctgacgcgcg
     1681 gcctcaatcg tctgtcccgc gccttccggc ggctgcatat actgcgttac cggttcggct
     1741 acggagaaga acatcaggtc gataccgatc ccggcagcaa acagcatcgc cgcccaactc
     1801 agcaggctga attccggttt ggattgttct ggcccgagct tcaccgaacc aaaacgcgaa
     1861 caagcgatac agaccacaaa gacaatatag agcgttgccg ccagtagata gtaccaaccg
     1921 aaggttttag aaacccagtc cagcgtgcgg ccaatccaca gggccgagaa gtcgcgaaac
     1981 aggatcgttg tcagggaaaa caacaaaatc agtccggcgg aggtgtaaaa caccaccgga
     2041 ttgattttgt ccttttccct gctgtgtgaa aggtctgtca tccagtatcc ccactgttat
     2101 tgttaccatt taaaatcaaa ttcgtaacaa ttaagacaca ttttatattg aacgtccaat
     2161 caataaccgc tttaatagat aaacaacgct gatgaatgga gtggcgaaaa tgcccaaatt
     2221 ggggatgcag tcgatccggc gcagacaact gatcgacgcc acactggaag caataaatga
     2281 agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc cgtgcaggcg tttctacggg
     2341 gatcatcagc cactatttca gggacaaaaa tggtctgctg gaagcaacca tgcgcgatat
     2401 caccagtcag ctgcgtgacg cggttttgaa tcgattacat gcacttccgc agggcagtgc
     2461 agagcagcga ttacaggcga ttgttggcgg aaacttcgat gaaacgcagg tgagcagtgc
     2521 ggcgatgaaa gcctggctgg cgttctgggc cagcagtatg catcagccga tgctctatcg
     2581 tttacagcag gtcagcagcc gccgcttgct gtcgaatctg gtgagcgagt ttcgccgcga
     2641 attgccgcgc gaacaggcac aggaagcggg ctacggcctt gccgcgctga ttgacggatt
     2701 atggctgcgc gcggcactga gcggcaaacc gctggataaa cctctcgcac actcgctgac
     2761 ccgtcacttt attactcagc atttacccac cgattaaccg aggagacgtg atgtcccgaa
     2821 tggcagaaca gcagctttat atacatggtg gttatacctc cgccaccagc ggtcgcacct
     2881 tcgagaccat taacccggcc aacggtaacg tgctggcgac cgtgcaggcc gccgggcgtg
     2941 aggatgtcga tcgcgccgtg aaaagcgccc agcaagggca aaaaatctgg gcggcgatga
     3001 ccgctatgga acgctcgcgt attctgcgtc gggccgttga tattctgcgt gaacgcaatg
     3061 acgaactcgc aaaactggaa accctcgaca ccggaaaagc atattcggaa acctcaaccg
     3121 tcgatatcgt taccggtgcg gacgtgctgg agtactacgc cgggctgatc ccgtcgctgg
     3181 aaggcagcca gatcccgttg cgtgagacgt catttgttta tacccgccgc gaaccgctgg
     3241 gcgtggtggc ggggattggc gcgtggaatt acccgatcca gattgccctg tggaaatccg
     3301 ccccggcgct ggcggcaggc aacgcaatga ttttcaaacc gagcgaagtc accccgctta
     3361 ccgcgttaaa gttggccgaa atttacagcg aagcgggcct gccggacggc gtatttaacg
     3421 tgttgccggg cgtgggcgcg gagaccgggc aatatctgac cgagcatccg ggcattgcca
     3481 aagtgtcatt taccggcggt gtcgccagcg gcaaaaaagt gatggctaac tcggcggcct
     3541 cttctctgaa agaagtgacc atggaactgg gcggtaaatc accgctgatc gttttcgacg
     3601 atgcggatct cgatctcgcc gccgatatcg ccatgatggc gaacttcttc agctccggtc
     3661 aggtgtgtac caatggcacc cgcgtcttcg ttccggcgaa atgcaaagcc gcatttgaac
     3721 aaaagattct ggcgcgcgtt gagcgcattc gcgcgggcga cgttttcgat ccgcaaacta
     3781 acttcggccc gctggtcagc ttcccgcatc gcgataacgt gctgcgctat atcgtcaaag
     3841 gcaaagagga aggcgcgcgc gtactgtgcg gcggcgatgt actgaaaggc gatggcctcg
     3901 ataacggcgc atgggtcgcg ccgactgtgt tcaccgattg cagcgacgaa atgaccattg
     3961 tgcgcgaaga gatcttcggg ccggtgatgt ccattctgac ctacgagtcg gaagaggaag
     4021 tcattcgccg cgctaacgat accgactacg gcctggcggc gggcatcgtg acggcggacc
     4081 tgaaccgcgc gcatcgcgtc attcatcagc tggaagcggg tatttgctgg atcaacacct
     4141 ggggcgaatc cccggcagag atgcccgttg gcggctacaa acactccggc attggtcgcg
     4201 agaacggcgt gatgacgctc cagagttaca cccaggtgaa gtccatccag gttgagatgg
     4261 ctaaattcca gtccatattc taaccaggag gtttatttgc aatttgacta catcattatt
     4321 ggtgccggct cagccggcaa cgttctcgct acccgtctga ctgaagatcc gaatacctcc
     4381 gtgctgctgc ttgaagcggg cggcccggac tatcgctttg acttccgcac ccagatgccc
     4441 gcagcgctgg cgttcccact acagggcaaa cgctacaact gggcctatga aacagagcct
     4501 gaaccgttta tgaataaccg ccgcatggag tgcggacgcg gtaaaggcct gggtggatcg
     4561 tcgctgatca acggcatgtg ctacatccgt ggcaatgcgc tggatctcga taactgggcg
     4621 caagaacccg gtctggagaa ctggagttat ctcgactgcc tgccctacta ccgcaaggcc
     4681 gagactcgcg atgtgggcga gaacgactac cacggtggcg atggcccggt gagcgtcacc
     4741 acctccaaac ccggcgtcaa tccgctgttt gaagcgatga ttgaagcggg cgtgcaggca
     4801 ggctacccgc gcacggacga tctcaacggc tatcagcagg aaggttttgg cccgatggat
     4861 cgcaccgtca cgccgcaggg ccgtcgcgcc agtaccgcgc gcggttatct cgatcaggcc
     4921 aaatcgcgcc ctaacctgac cattcgtact cacgctatga ccgatcacat catttttgac
     4981 ggcaaacgcg cggtgggcgt cgaatggctg gaaggcgaca gcaccatccc aacccgcgca
     5041 acggccaaca aagaagtgct gttatgtgca ggcgcgattg cctcaccgca gatcctgcaa
     5101 cgctccggcg tcggcaacgc tgaactgctg gcggagtttg atattccgct ggtgcatgaa
     5161 ttacccggcg tcggcgaaaa tcttcaggat catctggaga tgtatctgca atatgagtgc
     5221 aaagaaccgg tttccctcta ccctgccctg cagtggtgga accaaccgaa aatcggtgcg
     5281 gagtggctgt ttggcggcac tggcgttggt gccagcaacc actttgaagc aggtggattt
     5341 attcgcagcc gtgaggaatt tgcgtggccg aatattcagt accatttcct gccagtagcg
     5401 attaactata acggctcgaa tgcagtgaaa gagcacggtt tccagtgcca cgtcggctca
     5461 atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc gcgacccgca ccagcatccg
     5521 gcaattctgt ttaactacat gtcacacgag caggactggc aggagttccg tgacgcaatt
     5581 cgcatcaccc gggagatcat gcatcaaccg gcgctggatc agtatcgtgg ccgcgaaatc
     5641 agccccggca cggaatgtca gacggatgaa cagctcgatg agttcgtgcg taaccacgcc
     5701 gaaaccgcct tccatccgtg cggtacctgc aaaatgggtt acgacgagat gtccgtggtt
     5761 gacggcgaag gccgcgtaca cgggttagaa ggcctgcgtg tggtggatgc gtcgattatg
     5821 ccgcagatta tcaccgggaa tttgaacgcc acgacaatta tgattggcga gaaaatagcg
     5881 gatatgattc gtggacagga agcgctgccg aggagcacgg cgggatattt tgtggcaaat
     5941 gggatgccgg tgagagcgaa aaaatgagtc gtgatgtgaa ctaacgcagg aaccgatcag
     6001 attgagagtt accgttccag agagggggac cgaatcctta tataaacact gaggtaactc
     6061 tcatgcttca tatccagtat tcatgatgct ggctttttgc tgatatttaa cccgaacatt
     6121 cctgatacag actattaata caaccttatc tgcgacagat tttattttct ggatatatta
     6181 tgcgtgaaca aatcaaacag gatatcgatc tgattgagat tttattttat ctgaagaaaa
     6241 agattcgcgt tatccttttt attatagcta tctgtatggc aatggtgctg ttgtttctgt
     6301 atatcaataa agataatata aaagtgagtt acagcctaaa aataaaccag acaacaccag
     6361 gcatacttgt taactgtgat agcaataata actttgcctg tcagaccaca atgactgaag
     6421 atgttataca gcgaattact acattttttc acaccagccc agatgtcaag aacagagaaa
     6481 taaagctgga atggtcagga gataagagag atttaccaac tgctgaagcg gaaatatctc
     6541 gcgtgcaggc gtctattatc aaatggtatg cgtcagaata ccataatggc aggcaagttc
     6601 tcgatgagat acaaacacct tcagcaatta acagtgagct ttatacaaaa atgatatacc
     6661 tgaccaggaa ctggtcattg tatccgaacg gtgatggctg tgtaactata agctcaccag
     6721 aaataaaaaa taaataccct gctgccattt gcctggctct gggatttttt ctaagcattg
     6781 taatttctgt aatgttttgc cttgtcaaaa aaatggtaga tgaataccaa caaaactctg
     6841 ggtagtaata aacagcattc tgatagagag tgcctgtata aagctgaagt gacaatgacc
     6901 gggaagggaa cccgttttca ggaatggatg cctgtctttt tcactgtaaa tatcctggca
     6961 ttactaatgc caggatattt acagaagagc agcataacca tgacggaacg gggttaagtc
     7021 aggttgtcct taacgctatt ttcaacctcg tatgccttct tcaggtttat gttcagatct
     7081 catatctctc tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc
     7141 cgacgacttt gataagctca atatccgccg ttgaactagg gccgctaatg atgttaatgc
     7201 aggacggcat tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta
     7261 cacgcggcag gattgtgctt ttacgcagga taaaaagaga agattccggg agcaggctca
     7321 atgaacgccc gcgctcggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat
     7381 attcagcata cacgacaccc actttagcct gctctgcctg cgagatattc tcggcacctt
     7441 tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta atccccaatt
     7501 cctccagcct cgtgtcaccg ctaatcacga ccgactgatc tcccagctct ttacacagac
     7561 gtattgcagc ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc aacataacat
     7621 cgctggcaaa ctgaataaac gcgtcacagc gctgctgttg gttaagttgg gtaagccgct
     7681 cgttagcata gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac
     7741 ccagtgcctg agcaacgtta ttcaaaaatt cgcctcgatt atccattctt tttctcctgc
     7801 gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt
     7861 gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac
     7921 cagcttgccg catgagcgcc ggccatcatc ccgactttcc acaatcctgg atgactattg
     7981 gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc
     8041 atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgctg
     8101 tcacaagcag tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca
     8161 agtagcggag aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg
     8221 ccaatatggc gatatgccgg acaagtattc atacaagccc cgcagcgaat acagcgcagc
     8281 acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga
     8341 aactcttcag gaccatcaac gtgccccgct tcgcgcggtc ctgtcagcca ggtgttgtat
     8401 cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg
     8461 gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg
     8521 gtacacattc gcgcattacc ttcattggtc accaggcata ccgaacctgt ctctgccacc
     8581 gcgaaattac agccggtaat acctatttca gcactgagga aatcttcgcg gattttttgc
     8641 cggatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt
     8701 agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagatggc
     8761 ggatcttgat ccagctggag aatgtattca cccagatcgg tttcaatcac ctgaatgcca
     8821 gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc
     8881 accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgagtggc gtcttcttta
     8941 gtttttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga
     9001 taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc
     9061 tcccagtgcc ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg
     9121 tttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa
     9181 tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca acacttcagc
     9241 aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga tgttcagcag
     9301 gcaactcacg tcagcaccaa ttaaatactc agggcggact tccatcaggt gcacaacctt
     9361 ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc
     9421 gcagcaggta tcctgttcag caaaggtcaa cagctccagt ccacgcacat ttttcagcag
     9481 cgtaagtggc tcgtccttca ctcccagctt acgggccagg ctacaagatg ggtgatacac
     9541 agctctccct tgcaaactgg cacctacatc gactacccct aatttattaa caataaaaga
     9601 ggtgagatcc tgcatacgcg cggcaacctt ttcggcacgt gatgcccatt caggttcatc
     9661 cgccagatac gtcgggtaac tttttacggc ataggtgcaa gagccagccg gtgaaataat
     9721 gggatcgtcg ttatcctcca gtgcggcgat cagatttttc atccctggaa tcgcttcttt
     9781 gatataaccg ctattgatcg caggctgacc gcagcatccc tgtttctccg ggaaatttac
     9841 gcgacagccg agtttttcca gtagcagcac ggagtctcgt gccattcttg atttcagggc
     9901 gtcaccaata caggtgacaa agaaattgac attcacaact aatactccat tacttcatgc
     9961 ccatttatgc ccgatgtttc ccccccaccg ggggatcaat catcaataaa tatggcgttc
    10021 ttttttaatt ggctagcaac ataccacccc tcatttgtat gacaaatgat aatctttcat
    10081 ccaaacttca aatgcaataa ctaattttat ttctttcggc atcgctcaca ttttatgggg
    10141 aaatcgacgt tcatttttgg cataagaaat ccatttaaaa aggctacatc ccttatacct
    10201 attcagagct acttattcag gggttattga gatatacact gatggtgtac aaaaaaatca
    10261 ccgacaataa tcacgtatca agtaatagtc ggggttttat ttaattgaag caatttagtt
    10321 tgcattttta tttaaaaact gagctagagt ccatcagaaa aaaatatata aagcccaaac
    10381 caccgttgga aatagaaata gttgaaaggc tcacaactcc cggaatgcgg attatctttg
    10441 ccctgatttc tggcggtttt attcagggtg caagctgtct gaccctttcc cgatattctc
    10501 ccggagtaca accaaactcg cggacaaacg ccttgtgaaa agatgattca ctggcatagc
    10561 ctactgactc agcgatcacc acaacaggga gcatttcccg ggaaaacatc tgggccgcta
    10621 tttgtagacg caactttgtt aatacagcca gcggcgtggt tccggaaaca tcacggaaaa
    10681 gctgggcaaa acttgcccgg gacatgtgag cgatgctggc cagcgattcg acggtccagg
    10741 cgtgccctgg catttccagc atttgctgta ttaccgcacc aagacgtgga tgcagaagca
    10801 aactgagaat gtttttctca gtattaacct gtgcaatcca ctcacgcacc gcgagggtaa
    10861 agaatgtagc gcagatctgg ctacacagtg catccactcc cggcatcgcc gatctggatt
    10921 cctgttgtaa aaacgggatc gcctccctca gccagttata ttccacgctg tggttaaccg
    10981 gtgctaaaaa cagcgtttcc ggcaaagacg ttaaaaaata acgcgccgaa tgttgcaacc
    11041 gaagagtgcc gcagacaata caggtcgatt cattatcgac atgactcagg cgatgagctg
    11101 aattttgtgg tagcaggacc acatttcccg ggcgtaatgt aaaaatctct cccgtcggca
    11161 tttccagctt cgccgctcct tgcgttaacg catgccaacg aataaccgat aattccccgg
    11221 caccatgtgg aagctgccag tcacttccta acacgcaatt cttatcgatc gttccttgtg
    11281 gagcgttaag catcaacaaa cggctaaggg catccatatc agactcctga gtggtttttg
    11341 attacaaaca ccaacaatct tagtaaataa ctaatctaat accatcaaat ggttaatcat
    11401 gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa gacaaaatat
    11461 caagcgttgt gaagaaatgg tatttgctct tttgcgtcta tagtcatgat gtcaaatgaa
    11521 cgcgtttcga caggaaatca tcatgaataa atatcaggca gttattattg gttttggcaa
    11581 ggctggaaaa acattagccg tcacgctggc aaaagcaggt tggcgagtgg ctctcatcga
    11641 acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgcatcc caaccaaaac
    11701 attggttcat gacgcacagc agcacacaga ttttgtccgt gccatacagc gtaaaaatga
    11761 agtggttaat tttttacgta ataagaattt tcataatctt gcggatatgc ccaatatcga
    11821 cgtgatcgac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc atcggcctga
    11881 ggcaaatctg gaaattcatg gcgagaaaat ttttattaat accggtgcac aaaccgtggt
    11941 tccgccaatt cctggaatta ccaccacgcc aggagtatat gacagcaccg gattacttaa
    12001 tctaaaagaa ttgcctgggc atttaggtat tttgggcggc ggatatattg gcgttgagtt
    12061 cgcctctatg ttcgctaatt ttggcagcaa ggtaaccatt ttagaagcag cttcgctgtt
    12121 tttgcctcgg gaagatcggg atattgctga taatatcgcg acgattttac gcgatcaggg
    12181 cgtcgatatt atcctcaatg cccatgtgga gcgaatcagt caccatgaaa atcaagtgca
    12241 agtgcatagc gagcacgccc aactggcggt ggatgcactg ttaatagctt ccggtcgtca
    12301 accggctacc gcttcgttac atccagaaaa tgccggtatc gcagtaaacg agcgcggggc
    12361 aattgtcgtt gacaagcaat tacataccac cgcagaaaat atttgggcga tgggagatgt
    12421 taccggcggg ctgcaattta cttatatttc actggatgat taccgcattg tgcgtgatga
    12481 gttgctggga gaaggcagac gtagtactga tgatcggaaa aatgtgcctt attccgtatt
    12541 catgacaccg ccactgtcca gggttggtat gacagaagaa caggccagag agagtggtgc
    12601 tgatattcag gtggtgacat tgcctgtagc tgcaattccg cgtgccagag tgatgaatga
    12661 tactcgtggg gtattaaaag cgattgttga taataaaacc caacgtatat taggggcatc
    12721 actgctgtgt gttgactccc acgagatgat caatatagtg aaaatggtga tggatgccgg
    12781 gctgccttat agcatattac gcgatcagat atttactcat ccgtcgatga gcgaatcact
    12841 caatgatcta ttttcattag tcaaataaac tcaaaatcag acgccagaac aaatattctg
    12901 gcgtctcaga gaaaagaatc ttattaa
//
LOCUS       DAMZII010000136        12862 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZII010000136 DAMZII010000000
VERSION     DAMZII010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.136"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..197
                     /locus_tag="POA77_003851"
     CDS             <1..197
                     /locus_tag="POA77_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6367214.1"
                     /translation="GGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECPTVERLKM
                     LADERLYKSKKNGRAQISWQ"
     gene            complement(244..1569)
                     /gene="rimO"
                     /locus_tag="POA77_003852"
     CDS             complement(244..1569)
                     /gene="rimO"
                     /locus_tag="POA77_003852"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDI6367215.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            1782..2165
                     /gene="bssR"
                     /locus_tag="POA77_003853"
     CDS             1782..2165
                     /gene="bssR"
                     /locus_tag="POA77_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDI6367216.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2276..3391
                     /gene="yliI"
                     /locus_tag="POA77_003854"
     CDS             2276..3391
                     /gene="yliI"
                     /locus_tag="POA77_003854"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDI6367217.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRSTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3388..4014)
                     /gene="gstB"
                     /locus_tag="POA77_003855"
     CDS             complement(3388..4014)
                     /gene="gstB"
                     /locus_tag="POA77_003855"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDI6367218.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            4260..5462
                     /gene="dacC"
                     /locus_tag="POA77_003856"
     CDS             4260..5462
                     /gene="dacC"
                     /locus_tag="POA77_003856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI6367219.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(5509..6267)
                     /gene="deoR"
                     /locus_tag="POA77_003857"
     CDS             complement(5509..6267)
                     /gene="deoR"
                     /locus_tag="POA77_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDI6367220.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6325..6921)
                     /gene="ybjG"
                     /locus_tag="POA77_003858"
     CDS             complement(6325..6921)
                     /gene="ybjG"
                     /locus_tag="POA77_003858"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6367221.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7206..8438
                     /gene="mdfA"
                     /locus_tag="POA77_003859"
     CDS             7206..8438
                     /gene="mdfA"
                     /locus_tag="POA77_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDI6367222.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(8479..8763)
                     /locus_tag="POA77_003860"
     CDS             complement(8479..8763)
                     /locus_tag="POA77_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367223.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(8849..9664)
                     /gene="ybjI"
                     /locus_tag="POA77_003861"
     CDS             complement(8849..9664)
                     /gene="ybjI"
                     /locus_tag="POA77_003861"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDI6367224.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(9664..10872)
                     /locus_tag="POA77_003862"
     CDS             complement(9664..10872)
                     /locus_tag="POA77_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6367225.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            10956..11492
                     /gene="rcdA"
                     /locus_tag="POA77_003863"
     CDS             10956..11492
                     /gene="rcdA"
                     /locus_tag="POA77_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDI6367226.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(11597..12649)
                     /locus_tag="POA77_003864"
     CDS             complement(11597..12649)
                     /locus_tag="POA77_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6367227.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
BASE COUNT         3244 a         3154 c         3259 g         3205 t
ORIGIN      
        1 cgggtggaac gctggaagag ttgctatcga ttctaaaaga aatcgttcat ttccaggtgg
       61 gaagcattaa tttaagtacc agtatcggtg tagcacattc aaatgaatgt cctaccgtcg
      121 aacgcttgaa aatgctggcg gatgagcggc tgtataagag taagaaaaac ggcagggcac
      181 agattagctg gcagtaatca ttattcgcag gccggacaaa tgattttgcc cggcctgaat
      241 taattaaacc cggctacccc acaaatcgta ctcatcggcg tgctcgactt tcacacgcag
      301 gatatcaccc ggcttaacgt tggtttcacc attgagataa accgcgccgt cgatttccgg
      361 tgcatctgcc atgctgcgac caattgcgcc ttcttcgtcc acttcgtcga taatcaccag
      421 aatttcacgg cccactttct cttgcaggcg ctcggcggaa atctgctgct gcagctgcat
      481 gaaacggttc cagcgttctt ctttcacttc ttccggaacc tggtcaggca gggcattggc
      541 gtctgcacct tcaaccgggc tgtatttaaa gcagccaacg cgatccagac gcgcttcttt
      601 caggaagtcg agtagcatct ggaaatcttc ttctgtctcg ccagggaagc cgacaataaa
      661 ggttgagcgt agggtcagtt ccgggcagat ttcgcgccac tgtttgatgc gcgccagttg
      721 gcgatctaca gaacccggac gcttcatcag tttgagaatg cgcgggctgg cgtgctgcaa
      781 cggaatgtcc agatacggca ggattttgcc ttctgccatc agtgggatga cgtcgtccac
      841 atgcggataa gggtaaacgt agtgcagacg tgtccagatc cccagtttcg ataactgttc
      901 gcacaggctg accatgctgg tttttaccgg ctcgccgttg tggaagccag tacgatgttt
      961 aacatcaacg ccataggcgg aagtatcctg cgagatcacc agaatctctt taacgcccgc
     1021 atctaccaga cgtttcgctt cacttaacac ttcgccaatc ggacggctca ccaggtcgcc
     1081 gcgcatagac ggaataatgc agaaggtgca gcggtgatta cagccttctg aaattttcag
     1141 ataggcataa tgacgcggtg tcagtttcac accttgttct ggcaccaggc tcaggaatgg
     1201 gttgtgtttc ggttttggca cgtagtgatg aacgtgctcc agaacctgct catagctatg
     1261 aggcccggtg atttccagca ctttcgggtg gacttcgcgg atctgatctt cttttgcccc
     1321 cagacaaccg gtcacaatta ccttgccgtt ttcattcaac gcttcaccaa tggcttccag
     1381 tgattcttgt accgcgctgt caataaagcc gcaggtgttg acgatcacca tgtccgcatc
     1441 gtcatagctc ggtaccacgt cataaccttc agtgcgtagt tcggtgagaa tacgctctga
     1501 atcgacaagg tttttcggac agccaaggga aacaaagccg attttcggct ggggagttac
     1561 tttgctcata gcttaaaaaa tattcagtta caggaaaggt cagggcaggg attctacaga
     1621 gttctggata aaatttgtat cgcaatctca ttcgctggcg gaggcgaagg aaatgtaaat
     1681 tttgttaatt tggcgtgaag aattgatcct ggacagcatt ttgctcaaaa aatagccata
     1741 ctatttaatt gcaacaaggc tggaaagagg aggatcgaag tatgttcgtt gacagacagc
     1801 gaatcgatct gctgaaccgg ttgatcgacg cacgcgttga cctcgctgca tacgtgcaac
     1861 tgaggaaggc aaaaggatac atgtccgtca gcgaaagcaa tcatctacga gataactttt
     1921 ttaaactgaa tcgcgaactg cacgataaat cgctgcggtt gaatcttcat ctggatcagg
     1981 aagagtggag tgctcttcat catgctgaag aagcattagc gacagccgca gtatgtttga
     2041 tgagtgggca ccatgattgc ccgactgtta ttaccgtcaa cgccgataag cttgaaaatt
     2101 gtctgatgag cttaacgctg agtatccaga gcctgcagaa gcacgccatg cttgagaagg
     2161 cctgaaaact aagggggaga aagcgtctcc cccttcatgt ttaagttttt gtaaaaatga
     2221 atttgttatc tcctccactg actacgcttt aagccagagt caatccggag gcgttatgca
     2281 tcgacaatcc tttttccttg tgccccttat ttgtctttct tccgctctct gggcggctcc
     2341 tgcaacggta aatgtcgaag tactgcaaga caaactcgac catccctggg cactggcctt
     2401 tttacccgat aatcacggta tgttaatcac tctgcgcggc ggcgagttgc gtcactggca
     2461 agcaggaaaa ggattatctg cgccgctttc cggagttccg gacgtttggg cgcacgggca
     2521 gggcggcctg ctggacgtgg ttttagcgcc tgattttgct cagtctcgcc gcatctggtt
     2581 aagttattcc gaagttggcg atgatggcaa agccggaact gctgtgggtt atggccgctt
     2641 aagtgatgat ctctcaaaag tgaccgactt ccgcaccgtc tttcgccaga tgccaaaact
     2701 gtctaccggc aaccattttg gcgggcggct ggtattcgac ggtaaaggtt atctttttgt
     2761 tgctctgggc gaaaacaatc agcgctcgac ggcgcaggat ctggataaat tacagggcaa
     2821 actggtgcgt ctgaccgacc agggcgaaat cccggatgat aatcctttta taaaggaatc
     2881 cggtgtgcgc gccgagatct ggtcttatgg cattcgtaat ccgcaaggaa tggcgatgaa
     2941 tccgtggagt aatgcactgt ggctgaatga acatggcccg cgcggtggtg atgaaattaa
     3001 tatcccgcaa aaaggcaaaa actacggctg gccgctggca acctggggaa tcaactattc
     3061 aggctttaag ataccggaag cgaaagggga gatcgtcgcc gggaccgagc aacctgtttt
     3121 ttactggaaa gattcgcccg ctgtgagcgg catggccttc tataacagcg ataaattccc
     3181 ccagtggcag caaaaattat ttattggcgc gctgaaagat aaagatgtca ttgtgatgag
     3241 cgtcaacggc gacaaagtga cagaagatgg ccgtatttta acggacagag ggcagcgaat
     3301 tcgtgatgtt cgcactggac ccgacggtta tttatacgtt ctcaccgacg agtccagtgg
     3361 ggaattactt aaagttagcc cacgcaatta gctaacggga atcatcacca ctttgcgcac
     3421 tgcggggcgt tcagtgagtt gctgatacca gcgttgcaga tttggacgcg gtgtccaggt
     3481 caggccaacg ttaaacaaat tgtagatgaa cggtgcgata gcgatatcgc ccacaccaaa
     3541 ctcgtcgcca gagaaccatt ttacttttgc cagttccgca tcgagcaggg caaacagggc
     3601 gtcgcactct ttgcaactgg catcaatggc ggcctgatcg cgctcttccg gtggtgttct
     3661 gactaatccc atcaggatcc cgcgatgagc attactgagc gtctggtttg cccagtccat
     3721 ccatttttcc gcttccgcac gacgtgccgg tgagtcgatc cacagacgtt tttgcccgta
     3781 ctgtgccgcc agatagcgga caatggcgtt tgattcccaa agaatgagat cactttcgtc
     3841 gtcacgcaac aacggcacca gcccgttagg gttcatcgcc agaaaatcag catcgtgatt
     3901 tatcccaaac tcacggcccg cgagaatttg ctcataaggt agttccagtt cttcgagcgt
     3961 cagcaatact ttttttacgt tagttgaatt attccgaccc cacagcgtaa tcatactcac
     4021 cccttttccg acaggcagcc acaggctgaa caggactccg atggtgaagt aaagttaact
     4081 tttaagcaac agctggcaaa aaaatgacac cagaagcagc gaagcggcag gatattgcat
     4141 aaactttaaa aactttacca acttacggtt tctttaagtt tgtgtgtgcg ttattaatca
     4201 ccgaacttat catacggcga tataacgtat ttttttgaat ggatactcgg gtggcattta
     4261 tgacgcaata ctcctctctc cttcgtggtc ttgcagcggg ttctgcattt ttattccttt
     4321 ttgccccaac ggcattcgcg gcggaacaaa ccgttgaagc gccgagcgtg gatgcgcgtg
     4381 catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac gcggatgaga
     4441 aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg caggcgctta
     4501 aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc tgggcgacgg
     4561 gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat caggtttcgg
     4621 tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt attgcgctgg
     4681 ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt tatgccaaaa
     4741 aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg ccggggcagt
     4801 tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat gtgccggaag
     4861 agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag cctaaccgta
     4921 accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga accacggcag
     4981 gcgcgggata taatctggtt gcttcggcta cccagggcga tatgcgttta atctccgtag
     5041 tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta ttgacctggg
     5101 gtttccgctt ctttgaaacc gtgacgccaa ttaaacctga tgccaccttt gtgactcagc
     5161 gcgtctggtt tggtgataag agcgaagtga atcttggggc aggtgaagcg ggctcagtga
     5221 ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg gaaccgcagc
     5281 ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag cttaacggta
     5341 aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc ggattctttg
     5401 gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc tggttctctt
     5461 aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt aatacatcaa
     5521 cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac aatcactcac
     5581 cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc caaatttact
     5641 gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt ttaccggcaa
     5701 ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg cggaataaaa
     5761 agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatgggtt tgaaaatggc
     5821 gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt tctctttcag
     5881 cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat tatcaatcgc
     5941 ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agagtctgat cgggttctac
     6001 cagtgtcgca gccatttttg ccgcccggcg tttttcttcc accaggcggg atttttgatc
     6061 gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc cgagcaaaac
     6121 gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa ccccaagcag
     6181 ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca gcagctgccc
     6241 gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa gcgagccgct
     6301 caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga tcggtaatgc
     6361 aaaacagaag cgataccacg attgcagacg ttgatagagt ttatgcccca tcgcttgcca
     6421 gataatctga gcgctaaggc agccaatcat acctgccagc aatccaccga gcatatccag
     6481 cggccagtgc acgccaagat aaacgcgcga ccaggcaatg acgacggcca gcaccattaa
     6541 aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg tgaaaatcac
     6601 cgtaccgtgg tcgcttggga atgagtcatc cgccgcatga tgcaggaagt tatagccgat
     6661 attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg acacaaacag
     6721 gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg taagccccca
     6781 caaccaaagc accgcggcca gcagcggtac cacggtaatc aaatctttag caataaaaat
     6841 cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag agaatagaga
     6901 gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg agccaataca
     6961 ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg tcttatttcc
     7021 ctcaagcggc ctgtttacgg tgggtaattg taacgggcat aggttaaata aaacttaaag
     7081 aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt cagctacaaa
     7141 attcgctttc tcgttagctg tgcttttatt aaactctgcg cgattattat tggcgaagaa
     7201 attgcatgca aaataaatta gcttccggtg ccaggcttgg acgtcaggcg ttacttttcc
     7261 ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg attcaacccg
     7321 gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct acttcaatga
     7381 ccgcatatct ggcgggcggg atgtttttac aatggctcct ggggccgctg tcggatcgta
     7441 ttggtcgccg tccggtgatg ctggcggggg tggtgtggtt tatcatcacc tgtctggcaa
     7501 tattactggc gcaaaacatt gaacaattca ctctgttgcg cttcttgcag ggcataagcc
     7561 tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa gaggcggttt
     7621 gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta cttggtccgc
     7681 tggtgggcgc ggcgtggatc catgtgctgc cctgggaagg gatgtttgtc ttgtttgccg
     7741 cattggcagc gatctccttt ttcggtctgc aacaagccat gcctgaaacc gccacgcgta
     7801 taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg ctgaagaacg
     7861 gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg ctggcgtgga
     7921 tcgcccagtc gccgattatc atcattaccg gcgagcagtt gagcagctat gaatatggct
     7981 tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta gcgcgtctga
     8041 cctcgcgccg caccgtacgt tcgctgatta ttatgggcgg ctggccgatt attattggtc
     8101 tgttggtcgc tgctgcggca acggttatct catcgcacgc gtatttatgg atgaccgccg
     8161 ggttaagtat ttatgctttc ggcattggtc tggcgaatgc gggactggtg cgattaaccc
     8221 tgtttgccag cgatatgagt aaaggtacgg tttctgcggc gatgggaatg ctgcaaatgc
     8281 tgatctttac cgtcggtatt gaaatcagca aacatgcctg gctgaacggg ggcaacggac
     8341 tgtttaatct cttcaacctt gtcaacggaa ttttgtggct gtcgctgatg gttatctttt
     8401 taaaagataa acagatggga aattctcacg aagggtaaaa aaatgcctga ttgctttgtg
     8461 cgatcaggca ttctcgaatt aatggtgatg gtcgtcaatc tggtgttcga taaccatccc
     8521 ttcacctacg ctggcaagat ggcgaacata aggatgcggg cggtaagccg gagctggcgc
     8581 aggagccaca tagacggttt gcggcacagt cgcgacgctg accgcttgtg gaacgctgac
     8641 tgaatcagga accacaacca ctttgtatcc actcggcaca tcgacggtga cactttgcgc
     8701 catcgccaca ccagtaaagc ccattaaaag cgcgcccaac agtagacaat ttttcataaa
     8761 catttcgcct gaaataatgc agcgggttaa tttccgcgaa ttatgcagcc ttcattcagg
     8821 tattgtgttg ccgggcgtgc tgccgttttt attggtcaaa tggcgcttcg tgtttaagca
     8881 ctttatcgat cacatccagt acgccttcac ggttattgga gcctgcccgg tattttgccg
     8941 ctgcgacgac cgcattgccg gcattttcca ttgcaaaact aaagcctgcc tgacgcagca
     9001 tctcaatatc gttaccgcca tcgccaaaga ccaccacttc gctgtcgtct attccccata
     9061 atttctgcag ttggcgaagg ccattggctt tatgtacgcc ggggataatc agatcgatgc
     9121 tgccgttgcc ggtgtggacc ggcaccataa tatcgccgat ggcctcatgt aatgcttttt
     9181 gtacttgtgg aatcagttca tcggaaagat tcaggccaaa cttaaagaag atatcctcta
     9241 agttgtcaaa gttatcgacg tattccagac ggtgataata catttccgcc accgttttca
     9301 tggcatcgtc atattttttg agcgtatagg cgctattttt tccgcaggca ataatttcca
     9361 cttccgggcg cgtcagcaaa tgttccacga cagtagtaaa cgcatccttc gacagctcgc
     9421 cattaaaaac atctttgcct tcgctcacta cccagccgcc gttttcagcc acaaaggcaa
     9481 tttcattagc aatttcaggg aagaaagaga tcaactgata atattgattc ccgctggcga
     9541 ctacaaagcg aattccttgt gctttcattt gctgatactg agccataaac cgctcacggt
     9601 tataggtttt ttgatcgctt aagaaagtac catccatgtc taccgcaatt aatttaatgc
     9661 tcatcaacta ttctccatcg ccgtctgcgt tttggtatcg ggtttggcga cggctttcgc
     9721 gacaatagcc gcgagaataa ccagcgccag tacaaccagc attgcactac gtaatccata
     9781 atgttcgccg agatagccca gcagcggcgg cccgacgagg aaagccagat aaccggtcgt
     9841 tgctaccaca ctgacgcggg ttggtgcatc ggggccggta tcgctggcgg cagaaatggt
     9901 cagcgggaag cccagcgagg cacccagtcc ccagagtaca acagacaccc cagcgaccca
     9961 ggcgctatcg acaaaaataa tcagcccaat acccaacgcc cccattagtg cactggcccg
    10021 aaccacggca acgcgactgt aacggtcgat gaaccaaccg ccggtgaagc gtccaacggt
    10081 catccccagg gtaaaaccgg cataaatcag cgagccggaa gtagggctaa aaccgtgacc
    10141 gtcaaccatt aataagggta accagtcgtt ggcagaacct tcggcaaagg ccatcgccag
    10201 caccacaaca cctatcagca gcaactggat atcgcgataa aaaggaatgc ctttttcgcc
    10261 atgctgggtg ccatcggcag catttttgcc cgtaccgtca gggattgcct gaatggcgat
    10321 ataaataggc gcgataccta ccagcgccgc caataaaatg tgcaccgttg ccggaacgcc
    10381 aaaggccgtc agtgccatcc cgacaccagc gcctgccagc gtgcccaggc tataaaaacc
    10441 gtgcatcatt ggcaaaaccg ttttattcat ttctcgctca acggcggcac cttcaacgtt
    10501 tatcgccact tccgcagaac caaaacttgc cccaaagacg ccgagaccaa cggcaaagag
    10561 caggggcgat gtcagccaga gtgccagact taatatcatc atcccgatca atgcgcagga
    10621 catcgtgacc aggatgacat tacgcgtgcc aaagcgtttc actaaccacg ccgagcagag
    10681 aataccgctc atcgaaccga tcgacagacc aaagagaaca ccgcccattt cagcgatcga
    10741 gacagagaga atatcgcgga tagcaggcgt acgggttgcc caggacgcca ttaacaggcc
    10801 tggcaaaaag aagaacataa acagcgccca ggttcggcgt ttcaatgcat tacgtgaaga
    10861 atttacggtc atagatcacg tcaaaataag aagagggaag acaacattag caaggttgtg
    10921 tacatttgta cacaattgca gagagaggaa atgacatgcg tcgcgctaac gatccgcaac
    10981 ggcgagaaaa aattatccag gccacgctgg aggcggtgaa actttacgga atacatgctg
    11041 ttacgcaccg caaaattgct acccttgccg gggtaccgtt gggatcgatg acctactatt
    11101 tttcaggaat tgatgagttg ttgctggagg cgttcagcag ttttactgag attatgtccc
    11161 ggcaatatca ggcatttttt agcgatgtta gtgatgctca aggcgcatgc caggctatca
    11221 ccgatatgat ctacagctca caggttgcaa cgccggataa tatggagctg atgtaccagc
    11281 tctacgcgct ggctagccga aaaccgctat taaaaacggt aatgcaaaac tggatgcagc
    11341 gcagtcagca aacgctcgaa caatggtttg aacccggaac cgcccgcgcg cttgatgcgt
    11401 ttattgaggg gatgacgctg cattttgtca ccgaccgtaa gccgctatcg cgcgaggaga
    11461 ttttgaggat ggttgagagg gttgcagggt agtagataag ttttagataa caaaaaaccc
    11521 atttatgtaa acgggttaat gaaaacaata agttagagta aaaacaataa gttgggttgg
    11581 tggtcagtgg tggttattac ggggtaatgc caaccgctgc cgccactttg tcgccacttg
    11641 gcagcgttgc cagaggattg aaacggagcg ccgtttccag atgatccggt gccagatgtg
    11701 cgtaacgcat agtcattttt atatcgtggt gtccgagaat tttttgtaag gccagaatgt
    11761 ttccacccga catcatgaag tgcgccgcaa acgtatggcg cagaacgtgt gtgagttgac
    11821 cgcgagggag cacgatagac gttttttcca tcacggataa aaattgaaaa tagcagtctg
    11881 tgaagaaatt gaacccatca agcgccatga tctcttcgta aagctcttta ctgataggga
    11941 tgcttctgtt tttcttcccc ttcgttctta caaaggtaat tcggtatttg gtcacctgtg
    12001 agcgggtaag atttacggct tctcgccagc gtgcgcctgt gcttaagcat atcttaacta
    12061 ccagtgccag aattgggtcc tgacgtttgc aatcagccag taattcaaca atctgctcat
    12121 gggtaagcca tgccatctct ttttctgcga tggtgaattt tcgcatgttc tccagtgggt
    12181 tcggatacga ccattcgccc aggcgggata gttcgctaaa aacactactt agatagcttt
    12241 gctccaggtt aatggtgacc gggcttgctc ctttcttcca tttctcgctg aagtagatct
    12301 cacctgtcag gcgtttatct cgatagtggg caaacatttt agaggttaga tcagttgcaa
    12361 ggggattgcc cagagcgtca accatcagca gcaatttgtc atagacatgc tgcccagcag
    12421 tcagagattt accatgtagt ttgaaccata gctcaaccac gtctttcagt gttcgacgat
    12481 ccactgattc acccagccag ggctttgctt cggtttcttc catcgtgtga cgctcaaaag
    12541 ccagtgcttc gcctttggtg gcgaattgtt tacgcacacg acgcccacta cgtccggcgg
    12601 ggtaacattc gcaaagccat tttcctgtgg tgagttttcg tacagccata aaaaatgccc
    12661 tccagtggag agcattttta ctgtatatat aaccagtgtc aatgtatgaa tttgtatagc
    12721 catacatatc actgaagtaa aaaaactttt aaagcatctt tcacaatatt tgttggttca
    12781 ccaataccgc caccaccttt taaaaaatgg tattcactca tttctgccca ttgtcttttt
    12841 atattttcct cagcaggtaa aa
//
LOCUS       DAMZII010000137        12631 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZII010000137 DAMZII010000000
VERSION     DAMZII010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.137"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(34..1683)
                     /gene="actP"
                     /locus_tag="POA77_003865"
     CDS             complement(34..1683)
                     /gene="actP"
                     /locus_tag="POA77_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDI6367228.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1680..1994)
                     /locus_tag="POA77_003866"
     CDS             complement(1680..1994)
                     /locus_tag="POA77_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDI6367229.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2194..4152)
                     /gene="acs"
                     /locus_tag="POA77_003867"
     CDS             complement(2194..4152)
                     /gene="acs"
                     /locus_tag="POA77_003867"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI6367230.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVSDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4545..5981
                     /gene="nrfA"
                     /locus_tag="POA77_003868"
     CDS             4545..5981
                     /gene="nrfA"
                     /locus_tag="POA77_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDI6367231.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6026..6592
                     /gene="nrfB"
                     /locus_tag="POA77_003869"
     CDS             6026..6592
                     /gene="nrfB"
                     /locus_tag="POA77_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDI6367232.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6589..7260
                     /gene="nrfC"
                     /locus_tag="POA77_003870"
     CDS             6589..7260
                     /gene="nrfC"
                     /locus_tag="POA77_003870"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDI6367233.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7257..8213
                     /gene="nrfD"
                     /locus_tag="POA77_003871"
     CDS             7257..8213
                     /gene="nrfD"
                     /locus_tag="POA77_003871"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDI6367234.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8233..9951
                     /locus_tag="POA77_003872"
     CDS             8233..9951
                     /locus_tag="POA77_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDI6367235.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9944..10327
                     /gene="nrfF"
                     /locus_tag="POA77_003873"
     CDS             9944..10327
                     /gene="nrfF"
                     /locus_tag="POA77_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDI6367236.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10324..10920
                     /gene="nrfG"
                     /locus_tag="POA77_003874"
     CDS             10324..10920
                     /gene="nrfG"
                     /locus_tag="POA77_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDI6367237.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTALTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11262..12575
                     /gene="gltP"
                     /locus_tag="POA77_003875"
     CDS             11262..12575
                     /gene="gltP"
                     /locus_tag="POA77_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDI6367238.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         2812 a         3297 c         3537 g         2985 t
ORIGIN      
        1 ttccccggtc aggcgaccgg ggaaggggag agattaatgt gcgcggcctt gctcaacgcc
       61 aaagccggtc tgggagcgga taaactgtgc acggaacagt tcacgctcgc gcgcaccttc
      121 cgctgagtta tcggttgccg agaagaacca gatgccgagg aatgccacgc tgatagagaa
      181 cagcgccggg tattcatacg ggaagatggc tttttcgtga ccaaggatct gtacccaaat
      241 cgtcgggccg aggatcatca gtaccactgc ggtaatcagc cccagccagc cacccatcat
      301 cgcgccacga gtggtcagtt tcgaccagta catagaaagc agaatgatcg ggaagttaca
      361 gctcgctgcg atggcaaacg ccaggcctac catgaaggcg atgttctgat tctcaaacag
      421 cacgccgagg ataatggcaa tcacacctaa aatcagcacg gtgattttcg atacccgcag
      481 ttcttcacgt tcggtcgcgc cttttttgaa gacgttagcg tacaggtcat gcgaaaccgc
      541 tgatgcgccc gccagcgtca gaccagcaac caccgcaaga atggtggcaa aagcaaccgc
      601 tgaaataaaa ccgaggaaca ggttgccgcc cactgcattc gccaggtgaa cggccgccat
      661 gttgttacca ccaatcagat ggcccgccgc gtctttatat tccggattcg caccaaccag
      721 catgatcgcg ccgaagccga taataaaggt cagaatatag aagtagccca taaacccggt
      781 ggcgtagaac acgctcttac gtgcttcgcg ggcatcgctg actgtaaaga agcgcatcag
      841 aatgtgcggc aagcccgccg taccaaacat cagtcccaga cccagagaga gcgcggagat
      901 cggatctttc accagcccgc ccggcttcat gatgtcgaca cctttcgggt gtaccgccat
      961 cgcttcactg aacagattgt tgaagctaaa gccgacgtgt ttcatcacca taaaggccat
     1021 aaagctggca ccgaacagca acagcacggc tttgataatc tgtacccaag tggtcgccag
     1081 catgccgccg aacaggacgt acatcatcat cagcacgccg accagcacca ccgcaatgtg
     1141 atagttaagg ccaaacagca gctcgatcag tttacctgcg cccaccatct gggcgataag
     1201 gtaaagcgcc accaccacca gagaaccaca ggccgaaaga atacgaatcg gcccttgttt
     1261 cagacggtaa gaggccacat cggcaaaggt gtagcgcccc aggttacgca gacgttcggc
     1321 aatcaggaac aaaatgatcg gccagcccac caggaagccc agcgagtaaa ttaagccgtc
     1381 atagccggag gtaaacacca gcgcggagat ccccaagaat gaggcggcgg acatatagtc
     1441 cccggcaatc gccagcccgt tctggaagcc agtgatattg ccgcctgcgg tgtagtagtc
     1501 gctacgagaa cgtacgcgtt ttgatgccca gtaggtaatg ccgagcgtaa acacgacgaa
     1561 aatcaggaac ataataatcg cctgccagtt cgttggctgg cgctctacgg ccccgctaat
     1621 agcatccgcg gcgttagctg cgaaagggag tgtggcggca agcgccgtca gaactctttt
     1681 catgatgctt gtacctcatg caggacttca ttattaagac ggtcgaattc gccgttcgcc
     1741 cgccagatgt agataccggt gagaacaaag gagatcacaa tcactccaac accaattgga
     1801 ataccgcgtg tgacgctggt gttcggattc agcggcgtgc ccagccagcc gggcgcgaag
     1861 gcgatcagta aaataaagcc gatataaact gccagcataa taatcgacag gatggtggca
     1921 aaccgttgcc gtttttcgac taactccctg aaatgcgcat tgtcttctat ccgctgataa
     1981 atagtgccat tcatcacaga ttctccagag gtaatgtagg gattgtttta attcccgctc
     2041 ccttatggga gaaggttaac gctcgggtaa cccttgccga atgtaggccg gataaggcgt
     2101 ttacgccgca tccggcaatc aatgcctgat gcgacgctgt cgcgtcttat caggcctaca
     2161 aaccgttacc gactcgcatc cggcaattgt gggttacgat ggcatcgcga tagcctgctt
     2221 ctcttcaagc agcttctcga ctacgccagg atcggcaagc gtcgaggtat cgcccaggtt
     2281 gctggtatcg cccgccgcaa ttttgcgcag aatacggcgc ataattttgc cggaacgggt
     2341 tttaggcagg gagtcggtcc agtgcagcac gtctggcgtc gccagcgggc caatctcttt
     2401 acgcacccag ttgcggactt ctgcgtacag ttctggtgac ggttcctccc cgtgattaag
     2461 cgtgacgtag gcgtagatcg cctgaccttt aatattgtgc ggaataccta ctacggcggc
     2521 ttcggcaatc ttcggatgcg ccaccagcgc cgactcaatc tctgccgtcc ccagacggtg
     2581 accggagacg ttcagcacgt cgtccacacg cccggtgatc cagtaatagc catcttcatc
     2641 gcgacgcgcg ccgtcgccgc tgaaatacat atttttgaag gtggagaagt aggtctgttc
     2701 aaaacgttcg tgatcgccaa acagcgtacg cgcctgaccc ggccaggagt cggtgatcac
     2761 caggctgcct tcggtagcgc cttccagcgg gttaccttcg ttatcgacca gcgccggttg
     2821 cacgccgaag aacggacgtg ttgccgaacc ggctttcagc tcggtagcgc caggcagcgg
     2881 cgtgatcatg aaaccgccag tttcggtctg ccaccaggta tcgaccaccg gacatttctc
     2941 gttgccgatt tttttccagt accactccca cgcttctggg ttaattggct cgcccacgga
     3001 accgagaatg cgcagcgacg aacggtcggt accctcgatc gctttatcgc cttccgccat
     3061 cagcgcgcgg atcgccgtgg gtgcggtata gagaatattg acctgatgct tgtccaccac
     3121 ctgcgccata cgggcaggcg tcggccagtt gggtacgcct tcaaacatca gcgtggtcgc
     3181 accgcaggcc agcgggccgt acagcaagta actgtgtccg gtcacccagc ccacatcggc
     3241 ggtgcaccag tagatatcac ccggatgata atcaaagaca tatttaaagg tcagcgccgc
     3301 gtacaccaga taaccgccgg tagtatgcag cacacctttt ggcttaccgg tagaaccgga
     3361 ggtgtagaga ataaacagcg gatcttcggc gttcatctct tccgcctggt gctgatcgct
     3421 cgcttgctca accaggtcgt gccaccacag gtcgcgccct tcctgccagt caattttccc
     3481 gccagtacgc ttcagtacca ccacatgctc tacgctggtg acgttcgggt ttttcagcgc
     3541 gtcatcaacg tttttcttca gcggaatact gcgcccggca cgcacacctt cgtcggaagt
     3601 gatcaccagt cgtgagttgg aatcaataat gcgcccggca acggcttccg gcgagaagcc
     3661 gccgaaaatc accgaatgca ccgcgccaat gcgggcgcag gccagcatcg caaccgcggc
     3721 ttccggcacc atcggcatat aaatcgccac cacatcacct tttttaatgc ccagctcgag
     3781 cagggtattg gcgaagcggc agacgtcgcg gtgcagctct ttatagctga tatgtttgct
     3841 ctggctggcg tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt cttgcagatg
     3901 gcggtcaagg cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt taatggacac
     3961 attaccgggg gcaaaggagg tgtttttcac tttctggtaa ggtttgatcc agtcaagaat
     4021 ttttccctgt tcgccccaga aggtatcaga tacgttaata gattgttgat acatcgcctc
     4081 gtactgctga gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg
     4141 aatttggctc atgcttttgt tctccttgta ggatgttaat aatatgtggc ataagcgtta
     4201 aatgtagggg tattggcagt tttgtttagt atttgggcga cagatcacgc aaaagtagaa
     4261 ttgtgcaaat aaacggcagg gtaatttttg aaggtcacca acaaaagttg attaattctt
     4321 tgaggaacat gcagttatgc atgctgttga aaaagaggaa gatactgact aactctaaag
     4381 tggtatttta catgcactta caattgatta aagacaacat tcacagtgtg gttatttgtt
     4441 acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat
     4501 aaaaaccgcc attgcaacaa tggcgcaatt cggatgaagc ccctatgaca aggataaaaa
     4561 taaacgcacg ccgtatcttc agcttattaa ttcctttttt ctttttcact tctgttcacg
     4621 ctgaacaaac ggcagctccc gcaaaacctg taactgtgga agcgaagaat gaaacctttg
     4681 ccccgcagca tcccgatcaa tatctctcct ggaaagccac ctcggaacag tcagagcgtg
     4741 ttgacgccct ggcggaagat ccacggctgg tgatcctgtg ggcggggtat cccttctcgc
     4801 gcgattacaa caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc
     4861 gtaccggtgc gccgaaaaac gctgaagatg gtccgctacc gatggcatgc tggagttgta
     4921 aaagcccgga tgtggcgcgt ctgatccaga aagacggcga agatggctac ttccacggta
     4981 aatgggcgcg cggcggtccg gaaatcgtca acaacttagg ttgtgccgat tgccataaca
     5041 ccgcctcacc agagttcgcc aaaggcaagc cggagttaac cctttcccgt ccgtatgcgg
     5101 ctcgcgcgat ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat
     5161 cgatggtttg cggtcagtgc catgtggagt attacttcga cggcaaaaac aaagcggtta
     5221 aattcccgtg ggatgacggc atgaaagtcg aaaatatgga gcagtattac gacaaaattg
     5281 ccttctctga ctggactaac tccctgtcga aaacgccaat gctgaaagcg cagcacccgg
     5341 agtatgaaac ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc
     5401 atatgccaaa agtgcagaac gccgaaggca aactctacac cgaccataaa attggtaatc
     5461 cgtttgataa cttcgcccag acttgtgcga actgccatac ccaggacaaa gctgctttgc
     5521 aaaaagtggt cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag gttgaagatc
     5581 aactggttca cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg acggaagccg
     5641 aaatgaagcc gattcaggac gatatccgtc atgcccagtg gcgttgggat ctggcgatcg
     5701 cttcccacgg cattcatatg cacgcaccgg aagaaggttt acggatgctc ggtactgcga
     5761 tggataaagc ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca
     5821 cccatgaaat cgagatcccg gatatctcga ccaaagagaa agcccagcag gccattggcc
     5881 tgaacatgga acaaatcaag gccgagaagc aggacttcat caaaacggtg atcccgcagt
     5941 gggaagagca ggcacgtaaa aacggtctgt taagccaata accccgttcc gcctcgcaag
     6001 gggcggaaaa cacaatggag tgaatatgag cgtattacgt tcgttattaa ctgccggggt
     6061 gctggcgtcg ggcctgttgt ggagcctgaa cggaattacc gctacccctg cggcgcaggc
     6121 atctgatgat cgctacgaag ttacccagca gcgtaacccg gatgccgcct gtctggactg
     6181 tcataagcca gataccgaag gtatgcatgg aaaacatgct tccgttatca acccgaataa
     6241 caaactgccg gtcacctgca ccaactgtca cggccagcca tcaccgcaac accgcgaagg
     6301 ggtgaaagat gtgatgcgct ttaacgagcc gatgtacaag gttggagagc agaacagcgt
     6361 ctgtatgtcc tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca
     6421 cgtaaccaaa gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag atacgatgca
     6481 gacgttaagt gacaaaggac ggattaagat ttgcgtcgat tgccacagcg atcagcgcac
     6541 caatccgaac tttaacccgg cgtctgttcc gttgcttaag gagcagccat gacctggtct
     6601 cgtcgccagt ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc
     6661 gtggtggcga aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg
     6721 ttatgcatcg gctgtaccgc ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa
     6781 ggcgtctcgc gcctgacgat tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa
     6841 tatcgcttct tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc
     6901 ccgaccggtg cgtcttttcg tgatgccgct agcgggattg tcgatgttaa tcctgatctc
     6961 tgcgtcggtt gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg
     7021 gtcacgaaga cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca ggccggtaag
     7081 ctgcccgcgt gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa tctggacgat
     7141 cctaacagtg agatttcgca actgctgcgc cagaagccta cttaccgcta caagctggcg
     7201 ctgggaacca aaccgaagct gtaccgcgta ccgtttaaat acggggaggt gagccaatga
     7261 cgcagacttc cgcatttcat tttgaatcgc tggtgtggga ctggccgatt gccatctacc
     7321 tgtttttgat tggtatttct gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct
     7381 acccgcaggc gggcggtgca gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg
     7441 gcgcggtgat cctcggtctg ttgatcctcg tcttccacct gacaagaccg tggaccttct
     7501 ggaagctgat gttccactac agttttacct cggtgatgtc gatgggggtg atgctgtttc
     7561 agctctacat ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg
     7621 ccctgcaaca acgctggttg ccgaagctgg ggatcgtgca aaaggttctg agcctgctga
     7681 cgcccgttca tcgcggactg gaaacattga tgctggtgtt ggcggtgttg ttgggggctt
     7741 ataccggctt tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc
     7801 cggtgctgtt cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg
     7861 cgatacgcca acgcagtaac ccgcattcca cggaagcgca gtttgtacac cgtatggaaa
     7921 tccccgtggt atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg
     7981 gcgatgacgg taaagtgcgt gcgctggtgg cggcattagg tggcggtttc tggacgtggt
     8041 ggttctggct tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc aaaccgtggg
     8101 tcaatcgcag ttccggcatt cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg
     8161 tgttgatgct gcgctttttc attctctacg ccggacagtt gacggtggcg taagccagaa
     8221 aagaggtggt ttctggacgt attccttcct gaagtcggtt ttctggcgtt gttgttaagt
     8281 ctcggggtca acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct
     8341 gccatgatgc gactcacttg catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc
     8401 tttggcgtac tgacgtattg ttttctcatc agcgatttct cggtcattta tgtcgcgcaa
     8461 catagctaca gcctgctgtc gtgggaactc aaactggcgg cggtgtgggg cggtcatgaa
     8521 ggttcgctgc tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat
     8581 tatcggcagc aaaccgatcc gctatttccg ctgacgctag ccgttttatc tctcatgctc
     8641 gccgcactgc tactgtttgt agtgctgtgg tccgatccct tcgtgcggat atttccacca
     8701 gcaatcgaag gccgcgatct caatccgatg ctgcaacatc ccggtcttat ctttcatcca
     8761 ccgctgcttt accttggcta tggcggtttg atggtagcgg cgagcgtggc gctggcgagt
     8821 ttactgcgcg gcgagtttga tggtgcctgc gccagaattt gttggcgctg ggcactacct
     8881 ggctggagcg cattaacggc ggggatcatc ctcggttcct ggtgggccta ttgcgaactg
     8941 ggctggggcg gctggtggtt ctgggatccg gtggaaaacg cctctttatt accctggctt
     9001 tctgccactg cgctgctgca cagtttgtcc ctgacacgcc agcgggagat tttccgccac
     9061 tggtcgctgt tgctggcgat agtaactctg atgctgtcgc tactgggtac cttaattgtc
     9121 cgttctggca ttctggtttc ggttcatgcg ttcgcactgg ataacgtccg cgccgtgccg
     9181 ttgttcagcc tgtttgcact gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg
     9241 cgggacggtg gcccggtggt gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct
     9301 acgctgttgc tgttttgcgc agtgctactg atcgtgctgg tgggaacgct ttatccgatg
     9361 atttacggtc tgctgggctg gggacgcctc tccgttggcg cgccgtattt taaccgcgcg
     9421 acgttaccgt ttggcctgtt gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc
     9481 aaacgcgtgc agcttccggc gctggtagct catgcgggcg tgctgttatt tgccgctggg
     9541 atcgtggttt ccagcgtcag ccgtcaggag atcagcctga atttacagcc gggtcagcag
     9601 gtgacgctgg caggatacac cttccgtttt gagcgcctcg atctgcaagc caaaggcaat
     9661 tacaccagcg aaaaagcgat agtggcactg tttgaccatc agcaacgcat tggtgaactg
     9721 atgccggagc ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc gtcaattcgc
     9781 tggaacggca tccatgactg gtatgcggtc atgggtgaaa aaaccggagc ggatcgttac
     9841 gcttttcgct tgtatgtaca aagcggtgtg cgctggatct gggggggagg attgttgatg
     9901 attgcgggcg cattgttaag cggatggcgg gggaggaagc gcgatgaata aaggacttct
     9961 cacgctactg ctgttattta cctgttttgc gcgcgctcag gtcgtagaca cctggcaatt
    10021 cgccaatccg caacaacagc aacaggcgtt aaatattgcc agccagttac gttgtccgca
    10081 gtgccagaat caaaacttac tggaatccaa cgcgccggtg gcagtcagta tgcgccatca
    10141 ggtttacagc atggtggcgg aggggaagaa cgaagtcgaa atcattggct ggatgaccga
    10201 acgctacgga gattttgttc gctataaccc accgttaacg ggtcagacgc tggtgttgtg
    10261 ggcgctgcca gtggtgttgt tactgctgat ggcactgatc ctctggcgag tgagggcgaa
    10321 gcgatgaaag agcccaaaat accggtgaaa atgctgacaa cactcacgat tttgatggta
    10381 tttctctgtg tcggcagtta tctgttaagt ccaaaatggc aggcggtaca tgcggaatat
    10441 cagcgtcagc gcgatccgct acatcagttt gccagccagc aaaccccgga agcgcagctt
    10501 caggcattgc aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg
    10561 ggcgagtact atctgtggca aaacgattac agcaattcgc tgctggcgta ccgtcaggcg
    10621 ttgcaactac gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt gctttattac
    10681 caggccagcc agcatatgac cgccctgact cgcgcaatga tcgacaaagc cctcgcgctg
    10741 gactctaacg aaatcaccgc tctgatgctg ctggcttccg atgcgtttat gcaggcgaat
    10801 tacgcgcaag ccatcgaact atggcaaaaa gtgatggatc tcaactcacc ccggatcaac
    10861 cggacacagc tggtcgagtc gattaatatg gcgaaattgt tgcagcggag atcagattaa
    10921 tcatctggtg atgaagatta tgttattgag ttgtataaaa aataatcgaa tgaacatatg
    10981 ccaaaaataa tcaccaatca gtattattgc agattaaaca aataaaaatc tttccataac
    11041 aaatggttat tcattaatcc tgccataaac tcgcgttatt atgcattaat gcagcgaaaa
    11101 gctctgttgt taaagggttg cgcaacatac cgcgcaaatg atactgatca taagcgttaa
    11161 aaaaatctac aaaccaacgc aacacaattc atgccctggc agtatgtcac gttctcgcgt
    11221 ttctgaacgg ggaacggcgc tccattgagg aagttattct gatgaaaagt ataaaattca
    11281 gcctggcctg gcagattctg tttgctatgg tgctgggcat tctcctggga agctacctgc
    11341 actaccatag cgacagccgc gactggctgg tcgtcaattt gctctctccg gcgggtgata
    11401 tcttcatcca tctgatcaaa atgattgttg tgccgattgt gatctccacg ctggtggtgg
    11461 gtatcgcggg tgttggtgat gccaaacagc tcgggcgtat tggcgcgaaa accattattt
    11521 acttcgaggt gatcaccacc gtcgccatca ttttggggat cactctggcg aacgtcttcc
    11581 agcccggtgc cggggtggat atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga
    11641 gcactacgga agcggtacaa agcagttccc acggcattat gggcacgatt ttgtcgctgg
    11701 tgccgacgaa cattgtggcg tcgatggcga aaggcgaaat gctgccgatc atcttcttct
    11761 cggtgctgtt tggtctgggg ctttcctccc tgcccgcgac gcatcgtgaa ccgctggtga
    11821 ccgtgttccg ctccatctct gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg
    11881 caccggtggg tgtgtttgcg ctgattgcgg tgacggtggc taactttggt ttctcttctc
    11941 tgtggccact ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg
    12001 tagtgctggg aattgtggcg cgcctgtgcg ggttaagcgt ctggatcctg attcgtattc
    12061 tgaaagatga gctgattctg gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa
    12121 ttattgagaa gatggaagcc tacggagcac cggcgtcgat caccagcttc gtggtgccga
    12181 ccggttactc ttttaacctt gatggttcga cgctgtatca aagtattgcc gctattttca
    12241 tcgctcagct gtatggcatt gacctgtcca tctggcagga aatcattctg gtgctgacgc
    12301 tgatggtgac ctcgaaaggg attgctggcg tgccgggcgt gtcgtttgtg gtgctgctgg
    12361 caacgctggg tagcgtaggt attccgctgg aaggcctggc gtttattgct ggtgttgacc
    12421 gtatcctcga catggcgcgt actgcgctga acgtggtggg taatgcgctg gcggtgctgg
    12481 tgattgccaa gtgggaacac aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag
    12541 tgctgggcaa atttgataaa actgcggatc aataattgaa gattgccggg gttatccacc
    12601 cggcaatgtg tggatgcctg atgcgacgct g
//
LOCUS       DAMZII010000138        12542 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZII010000138 DAMZII010000000
VERSION     DAMZII010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.138"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(84..1367)
                     /gene="gltA"
                     /locus_tag="POA77_003876"
     CDS             complement(84..1367)
                     /gene="gltA"
                     /locus_tag="POA77_003876"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDI6367239.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1573..1680)
                     /locus_tag="POA77_003877"
     CDS             complement(1573..1680)
                     /locus_tag="POA77_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367240.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2061..2465
                     /gene="sdhC"
                     /locus_tag="POA77_003878"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2061..2465
                     /gene="sdhC"
                     /locus_tag="POA77_003878"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI6367241.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2459..2806
                     /gene="sdhD"
                     /locus_tag="POA77_003879"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2459..2806
                     /gene="sdhD"
                     /locus_tag="POA77_003879"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDI6367242.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2806..4572
                     /gene="sdhA"
                     /locus_tag="POA77_003880"
     CDS             2806..4572
                     /gene="sdhA"
                     /locus_tag="POA77_003880"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDI6367243.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4588..5304
                     /gene="sdhB"
                     /locus_tag="POA77_003881"
     CDS             4588..5304
                     /gene="sdhB"
                     /locus_tag="POA77_003881"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDI6367244.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5505..8306
                     /gene="sucA"
                     /locus_tag="POA77_003882"
     CDS             5505..8306
                     /gene="sucA"
                     /locus_tag="POA77_003882"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI6367245.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8321..9538
                     /gene="odhB"
                     /locus_tag="POA77_003883"
     CDS             8321..9538
                     /gene="odhB"
                     /locus_tag="POA77_003883"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI6367246.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9632..10798
                     /gene="sucC"
                     /locus_tag="POA77_003884"
     CDS             9632..10798
                     /gene="sucC"
                     /locus_tag="POA77_003884"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI6367247.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10798..11667
                     /gene="sucD"
                     /locus_tag="POA77_003885"
     CDS             10798..11667
                     /gene="sucD"
                     /locus_tag="POA77_003885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI6367248.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11771..12493)
                     /gene="mngR"
                     /locus_tag="POA77_003886"
     CDS             complement(11771..12493)
                     /gene="mngR"
                     /locus_tag="POA77_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDI6367249.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3012 a         3203 c         3397 g         2930 t
ORIGIN      
        1 acaatttttt gtgtttaggc atataaaaat taacccgcca tttgaacggc gggttaaaat
       61 atttacaact tagcaatcaa ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca
      121 tatcctgtat acagctgacg cggacgggca atcttcatac cgtcactgtg catttcgctc
      181 cagtgggcga tccagccaac ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac
      241 ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg gtacagtttc
      301 ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc
      361 aggtcatctt tggtgcccag ctctttcaga acttcatggc aggtttcacg cattacggtg
      421 gcgcgcgggt cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca
      481 tttttatctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct aatttcttcc
      541 agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag tgaagcaata
      601 cctgctgcga tacaggcaaa cgggttcgca cccgaagagc cagcggtacg cacggtggag
      661 gtagaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc
      721 agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt caggaagtta
      781 ccggcatagg agagatcgtt gcgcggataa acaaatggct gaccaatgga atacttgtaa
      841 cacatcgcgg ccatggtcgg cattttcgac agcaggcgga acgcggcaat ttcacgatga
      901 cgaggattgt taacatccag cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca
      961 cacatgactg ccattggatg tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc
     1021 tcgtggatca tggtatgacg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc
     1081 ggtttttcac cattcagcag gatgtaacaa acttccaggt agttagaatc ggtcgccagc
     1141 tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt
     1201 ttagattcgc aggatgcggt tgaagtgaag cctgggtcaa aggtgaacac accttttgaa
     1261 ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca
     1321 acagctgtgt ccccgttgag ggtgagtttt gcttttgtat cagccattta aggtctcctt
     1381 agcgccttat tacgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt
     1441 tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct
     1501 ctgggcgctt gcaggtaaag gatccattga tgacgaataa atggcgaatc aagtacttag
     1561 caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac
     1621 ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat
     1681 tatatgcttt tcctggtaat gtttgtaaca actttgttga atgattgtca aattagatga
     1741 ttaaaaatta aataaatgtt gttatcgtga cctggatcac tgttcaggat aaaacccgac
     1801 aaactatatg taggttaatt gtaatgattt tgtgaacagc ctatactgcc gccaggtctc
     1861 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
     1921 gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg
     1981 gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc
     2041 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
     2101 gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc
     2161 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg
     2221 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
     2281 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta
     2341 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
     2401 ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
     2461 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc
     2521 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ccaccagtgg
     2581 cgaactgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac
     2641 cctgctggcg ctgttttcta tcttgattca tgcctggatc ggcatgtggc aggtgttgac
     2701 cgactacgtt aaaccgctgg cattgcgcct gatgctgcaa ctggtgattg tcgttgcact
     2761 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
     2821 agagaatttg atgcagttgt gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa
     2881 atttcccaga gcggccagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat
     2941 accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg
     3001 gaatggcata tgtacgacac cgtaaaaggg tcggactata tcggtgacca ggacgcgatt
     3061 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tcgaacacat gggcctgccg
     3121 ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
     3181 ttcggcggcg agcaggcggc acgcactgcg gcggcggctg accgtaccgg tcacgcactg
     3241 ttgcacacgc tttatcagca gaacctgaaa aaccacacca ccattttctc cgagtggtat
     3301 gcgctggatc tggtgaaaaa ccaggatggc gcggtggtgg gttgtaccgc actgtgcatc
     3361 gaaaccggtg aagtggttta tttcaaagcc cgcgctaccg tgctggcgac tggcggggca
     3421 gggcgtattt atcagtccac cactaacgcc cacattaaca ctggcgacgg cgtcggcatg
     3481 gctatccgcg caggcgtacc ggtgcaggat atggaaatgt ggcagttcca cccgaccggt
     3541 attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg
     3601 aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga cctggcgggc
     3661 cgtgacgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt
     3721 ccgtgggggc cacacgcgaa actgaaactc gaccacctgg gtaaagaagt tctcgaatcc
     3781 cgcctgccgg gtatcctgga actctcccgt accttcgctc acgttgatcc ggtgaaagag
     3841 ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc
     3901 ggtcaggcgc tgacggtgaa cgagaaaggc gaagatgtgg ttgttccggg actgtttgcc
     3961 gttggtgaaa tcgcttgtgt atcggtacac ggcgctaacc gtctgggcgg caactcgctg
     4021 ctggacctgg tggtctttgg tcgtgcagcg ggtctgcatc tgcaagagtc tatcgccgaa
     4081 cagggcgcat tgcgcgatgc cagcgagtct gatgttgaag catctctgga tcgtctgaac
     4141 cgctggaaca ataatcgtaa cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa
     4201 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
     4261 cagttgaaag tgatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag
     4321 ttcaacaccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
     4381 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc
     4441 ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgccaga gtcggaatcc
     4501 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
     4561 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
     4621 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgc
     4681 gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc
     4741 cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat
     4801 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
     4861 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
     4921 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
     4981 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggctgtatga atgtattctc
     5041 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
     5101 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
     5161 agccgcctcg acggtttgag cgatgcattc agcgtattcc gctgtcacag catcatgaac
     5221 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg
     5281 atgttgttgc aacgtaatgc gtaattaaat gttgatgccg gggaaatccc ccggctattc
     5341 aggaaatctt taaaaactgc ccctgacact aagacagttt ttaaaggttc cttcgcgagc
     5401 cactacgtag acaagagctc gcaagtgaac cccggcacgc acataactgt gcgtggtagt
     5461 atccacggcg aagtaagcat aaaaaagatg cttaagggat cacgatgcag aacagcgctt
     5521 tgaaagcctg gttggactct tcttacctct ctggcgcaaa ccagagctgg atagaacagc
     5581 tctatgaaga cttcttaacc gatcctgact cggttgacgc taactggcgt tcgacgttcc
     5641 agcagttacc tggtacggga gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt
     5701 tccgccgcct ggcgaaagac gcttcacgtt actcttcaac gatctccgac cctgacacca
     5761 atgtaaagca ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc
     5821 atgcgaatct cgatccgctg ggactgtggc agcaagataa agtggccgat ctggatccgt
     5881 ctttccacga tctgaccgaa gcagacttcc aggagacctt caacgtcggt tcatttgcca
     5941 gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc cctcaagcaa acctactgcg
     6001 gcccgattgg tgccgagtat atgcacatta ccagcaccga agaaaaacgc tggatccaac
     6061 agcgtatcga gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg
     6121 aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc aaaattccct ggcgcaaaac
     6181 gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct taaagagatg atccgccacg
     6241 ctggcaacag cggcacccgg gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg
     6301 tgttggtgaa cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac
     6361 ataaagaaca cctcggcacg ggtgacgtga aataccacat gggcttctcg tctgacttcc
     6421 agaccgatgg cggcctggtg cacctggcgc tggcgtttaa cccgtctcac cttgagattg
     6481 taagcccggt cgttatcggc tctgttcgtg cccgtctgga cagacttgat gagccgagca
     6541 gcaacaaagt gctgccaatt accatccacg gtgacgccgc agtgaccgga cagggcgtgg
     6601 ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga agttggcggt acggtacgta
     6661 tcgttatcaa caaccaggtt ggtttcacca cctctaatcc gctggatgcc cgttctacgc
     6721 cgtactgtac tgatatcggc aagatggttc aggcaccgat tttccacgtt aacgcggacg
     6781 atccggaagc cgttgccttt gtgacccgtc tggcgctcga tttccgtaac acctttaaac
     6841 gtgatgtctt catcgacctg gtatgctacc gccgtcacgg ccacaacgaa gccgacgagc
     6901 cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa acatccgaca ccgcgcaaaa
     6961 tctacgctga caagctggag caggaaaaag tggcgacgct ggaagatgcc accgagatgg
     7021 ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt ggtagcagag tggcgtccga
     7081 tgaacatgca ctctttcacc tggtcgccgt acctcaacca cgaatgggac gaagagtacc
     7141 cgaacaaagt tgagatgaag cgcctgcagg aactggctaa acgcatcagc acggtgccgg
     7201 aagcggttga aatgcagtct cgcgttgcca agatttatgg cgatcgccag gcgatggctg
     7261 ccggtgagaa actgttcgac tggggcggcg cggaaaacct cgcttacgcc acgctggttg
     7321 acgaaggcat tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc
     7381 gccacgcggt gatccacaac cagtctaacg gttccactta cacgccgctg caacacatcc
     7441 ataacggcca gggcgcgttc cgtgtttggg actccgtact gtctgaagaa gcagtgctgg
     7501 cgtttgaata cggttatgcc accgcagaac cacgcaccct gaccatctgg gaagcgcagt
     7561 tcggtgactt cgccaacggt gcgcaggtgg ttatcgacca gttcatctcc tctggcgagc
     7621 agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc gcacggttac gaagggcagg
     7681 ggccggagca ctcctccgcg cgtctggaac gttatctgca actttgtgct gagcaaaaca
     7741 tgcaggtttg cgtaccgtct accccggcac aggtttacca catgctgcgt cgtcaggcgc
     7801 tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc
     7861 tggcggtttc cagcctcgaa gaactggcga acggcacctt cctgccagcc atcggtgaaa
     7921 tcgacgagct tgatccgaag ggcgtgaagc gcgtagtaat gtgttctggt aaggtttatt
     7981 acgacctgct ggaacagcgt cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg
     8041 agcaactcta cccgttcccg cataaagcga tgcaggaagt gttgcagcag tttgctcacg
     8101 tcaaggattt tgtctggtgc caggaagagc cgctcaacca gggcgcatgg tactgcagcc
     8161 agcatcattt ccgtgaagtg attccgtttg gggcttctct gcgttatgca ggccgcccgg
     8221 cctccgcctc tccggcggta gggtatatgt ccgttcacca gaaacagcaa caagatctgg
     8281 ttaatgacgc gctgaacgtc gaataaataa aggatacaca atgagtagcg tagatattct
     8341 ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc
     8401 cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact
     8461 ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac
     8521 ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac
     8581 cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa cgccagcagg cgtctctgga
     8641 agagcaaaac aacgatgcgt taagcccggc gatccgtcgc ctgctggctg aacacaatct
     8701 cgacgccagc gccattaaag gcaccggtgt gggtggtcgt ctgactcgtg aagatgtgga
     8761 aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg atccggcggc
     8821 gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg atgactcgcc tgcgtaagcg
     8881 tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga
     8941 agtcaacatg aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg
     9001 ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa
     9061 acgttacccg gaagtgaacg cgtctatcga cggcgatgac gtggtttacc acaactattt
     9121 cgacgtcagc atggcggttt ctacgccgcg cggcctggtg acaccggtac tgcgtgatgt
     9181 cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggcag tcaaaggccg
     9241 tgacggcaag ctgaccgttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg
     9301 tgtgttcggt tccctgatgt ctacgccgat catcaacccg ccgcagagcg caattctggg
     9361 tatgcacgct atcaaagatc gtccgatggc ggtgaatggt caggttgaga tcctgccgat
     9421 gatgtacctg gcgctgtcct acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt
     9481 cctggtaacg atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta
     9541 gtttaagctt cacctgcact gtagaccgac gtggataggg tgtcggtcta cggtttaaaa
     9601 gataacgatt actgaaggat ggacagaaca catgaactta catgaatatc aggcaaaaca
     9661 actttttgcc cgctatggct taccagcacc ggtgggttat gcctgtacta ctccgcgcga
     9721 agcagaagaa gccgcttcaa aaatcggtgc cggcccgtgg gtagtgaaat gtcaggttca
     9781 cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta aacagcaaag aagacatccg
     9841 tgcttttgca gaaaactggc tcggcaagcg tctggtaacg tatcaaacag atgccaatgg
     9901 ccaaccggtt aaccagattc tggttgaagc agcgaccgat atcgctaaag agctgtatct
     9961 cggtgccgtt gtagaccgta gttcccgtcg cgtggtcttt atggcctcca ccgaaggcgg
    10021 cgtggaaatc gaaaaagtgg cggaagaaac tccgcacctg atccataaag ttgcgcttga
    10081 tccgctgaca ggcccgatgc cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga
    10141 aggtaaactg gttcagcagt tcaccaaaat cttcatgggc ctggcgacca ttttcctgga
    10201 gcgcgacctg gcgttgatcg aaatcaaccc gctggtcatc accaaacagg gcgatctgat
    10261 ttgcctcgac ggcaaactgg gcgctgacgg caacgcactg ttccgccagc ctgatctgcg
    10321 cgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca caggctgcac agtgggaact
    10381 gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt aacggcgcag gtctggcgat
    10441 gggtacgatg gacatcgtta aactgcacgg cggcgaaccg gctaacttcc ttgacgttgg
    10501 cggcggcgca accaaagaac gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa
    10561 agtgaaagcc gttctggtta acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga
    10621 cggtatcatc ggcgcggtag cagaagtggg tgttaacgta ccggtcgtgg tacgtctgga
    10681 aggtaacaac gccgaactcg gcgcgaagaa actggctgac agcggcctga atattattgc
    10741 agcaaaaggt ctgacggatg cagctcagca ggttgttgcc gcagtggagg ggaaataatg
    10801 tccattttaa ttgataaaaa caccaaggtt atctgccagg gctttactgg tagccagggg
    10861 actttccact cagaacaggc cattgcatac ggcactaaaa tggttggcgg cgtaacccca
    10921 ggtaaaggcg gtactaccca cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt
    10981 gctgctactg gcgctaccgc ttctgttatc tacgtaccag caccgttctg caaagactcc
    11041 attctggaag ctattgatgc aggcatcaaa ctgattatca ccatcactga aggcatcccg
    11101 acgctggata tgctgactgt gaaagtgaaa ctggacgaag ccggcgttcg tatgatcggc
    11161 ccgaactgcc cgggcgttat cactccgggt gaatgcaaaa tcggtatcca gccgggtcac
    11221 attcacaaac cgggtaaagt gggtatcgtt tcccgttccg gtacactgac ctatgaagcg
    11281 gttaaacaga ccacggatta cggtttcggt cagtcgacct gtgttggtat cggcggtgac
    11341 ccgatccctg gctctaactt tatcgacatt ctcgaaatgt tcgaaaaaga tccgcagacc
    11401 gaagcgatcg taatgatcgg tgagatcggc ggtagcgctg aagaagaagc ggcagcgtac
    11461 atcaaagagc atgttaccaa gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa
    11521 ggcaaacgta tgggccacgc gggtgccatc attgccggtg ggaaagggac tgcggatgag
    11581 aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt
    11641 gaagcactga aaactgttct gaaataaata tctgtaataa gaaatagccc tcgccgcttc
    11701 cctctacagg aatggcgaag ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc
    11761 ttttttgtgc ttatcgcgat gattttcgtt gcgctatcag ggtaaattta tagtcatcgg
    11821 tattaaaagc gttgcggcta tattcaaaca cccgaccatc aactaaatat ccacgcgata
    11881 ctttttcaag aatcggcttt gtctggctga tattaagcag acggctcatc tcttcggttg
    11941 gcatcagagg aatgatttcc tgttcgctac gatcgataac cattttcttc acttcttcaa
    12001 taaagtgata ttttgaattt tccatgacct gccaggtgag atccgggaac aacgcaagcg
    12061 gcatccaggt ttcttccagc gccattggct tttgcttgcg atagcgcacg cgcttcacat
    12121 gccacacacg atcctgcgcg gtgatttgta gctgttgctg aagaaaatcg tcagccggaa
    12181 tcacttcaaa tatcaggact tcactgtgtg tatcgacgtg acggtccgac agtttttcat
    12241 caaaactggt taactgaaaa atatcgtaat tgacccgctc ttctttgacg taagtcccgc
    12301 tgccctgaat gctttcgagg atctgttgct cgactaactg gcgcaaagcc tgacgcaccg
    12361 taacccggct gacgccaaac tctgtttgta atgccgattc ggtaggtaac gcatcgccag
    12421 gtttaagctc gccacgcgca atttgttcac gaatgcgatc ggcaatctgc cggtataagg
    12481 gcttgtgtcc catttttagt atctcattaa tacgaattta gccattatgc ccgataaatt
    12541 ca
//
LOCUS       DAMZII010000139        12532 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZII010000139 DAMZII010000000
VERSION     DAMZII010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.139"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(44..325)
                     /locus_tag="POA77_003887"
     CDS             complement(44..325)
                     /locus_tag="POA77_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDI6367250.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(588..1697)
                     /gene="apbC"
                     /locus_tag="POA77_003888"
     CDS             complement(588..1697)
                     /gene="apbC"
                     /locus_tag="POA77_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDI6367251.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1829..3862
                     /gene="metG"
                     /locus_tag="POA77_003889"
     CDS             1829..3862
                     /gene="metG"
                     /locus_tag="POA77_003889"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI6367252.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4003..7797
                     /locus_tag="POA77_003890"
     CDS             4003..7797
                     /locus_tag="POA77_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDI6367253.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLFTIIQEHPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCTQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHACADLLPAIV
                     VSPPWLSKKKKATIPVLELAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPAALGKADEAQDNARAALRMLSEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     RESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSTENQLLTCFRVAEDNSYST
                     ADDDLFTLPEGDISVGIPHVLEISPTDAAAFGQLFADYKLLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7807..11439
                     /locus_tag="POA77_003891"
     CDS             7807..11439
                     /locus_tag="POA77_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDI6367254.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAVLACIAQ
                     KDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRFIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11500..11820
                     /locus_tag="POA77_003892"
     CDS             11500..11820
                     /locus_tag="POA77_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367255.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
BASE COUNT         3298 a         3185 c         3044 g         3005 t
ORIGIN      
        1 atgatttcgg ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta
       61 acacgattcg acgtttgcta tcgctacctg caacaaccac ttctgtcgta acacctttga
      121 cgggcgaaga aacctgatga cgggtgtcaa cattatacgt taactgacca caatgctgtt
      181 gtggttgact attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg
      241 ttaatgtggc agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa
      301 ttcctgacag ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga
      361 tagaatgagt gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata
      421 atgaagcatt gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa
      481 tattcgcagt attaaataag tgttcattaa tgcggcaaaa actaatacac cgcatcaatg
      541 taacatctct gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg
      601 aaggaaatct cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca
      661 gccagctggc ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg
      721 cctttatcga gatcctcacg taaggaaatg tgtaacggca tctgccccag cagctgtgtg
      781 tggtattttt cagccagttt ctctgcacca ccggtaccga aaatcggttc atggtgacca
      841 cagttactgc aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact
      901 ttctcgaaca tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg
      961 accacgactg caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg
     1021 cctggcggca tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc
     1081 tgcattaacg ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc
     1141 agataaccaa ttgaattggt tgccaggcca tgagacataa tcggtgccat atgggtaccg
     1201 tccggcgaag ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag
     1261 atatcggcgt ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt
     1321 accgccgtgg aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc
     1381 acaccattaa tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt
     1441 ttccagtcga tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt
     1501 aactcttcaa acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg
     1561 tccatccagg cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg
     1621 tgctgaaaat tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc
     1681 tgggattgtt cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt
     1741 acctgaagaa tatagagaag tacttactta acattttccc atttggtact atctaacccc
     1801 ttttcactat taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac
     1861 gtgcgcactg ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca
     1921 ggctgatgtc tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc
     1981 cgacgatgcc cacggtacac cgatcatgct gaaagctcag cagcttggta tcaccccgga
     2041 gcagatgatt ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag
     2101 ctatgacaac tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta
     2161 ctctcgcctg aaagagaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc
     2221 ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc
     2281 cccggatcaa tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt
     2341 gatcgagccg aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt
     2401 cttctttgat ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt
     2461 gcaggagcag gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga
     2521 tatctcccgc gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt
     2581 ctacgtctgg ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa
     2641 gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta
     2701 ccacttcatc ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga
     2761 aggcagcaac ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg
     2821 cgcaaagatg tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt
     2881 tgacgctgac agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat
     2941 cgatctcaac ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt
     3001 taacctggcc tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag
     3061 cgaattggct gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga
     3121 agcgtgggaa agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct
     3181 ggctaaccgc tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga
     3241 tgccgacctg caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta
     3301 cctgaagccg gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact
     3361 gacctgggat ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct
     3421 gtataaccgc atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt
     3481 gaaagcagct gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat
     3541 cacctttgac gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt
     3601 tgttgaaggt tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa
     3661 tgtcttctcc ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac
     3721 cattatggtg gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt
     3781 aatggctgct ggtcctggcg ggaaagatat cttcctgtta agcccggatg ctggtgctaa
     3841 accaggccat caggtgaaat aatccccctt taaggcgctg catcgacagc gccttttctt
     3901 tataaattcc taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg
     3961 gtagcatctg cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat
     4021 caggacgaaa aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc
     4081 agttggggaa aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg
     4141 gcagtggcaa aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag
     4201 caagcgaagg ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg
     4261 gacttattca ccattattca ggaacacccc tctttcgtag cagaaacccg tgcgcctgac
     4321 aaaaatacag atgctgtttt accgtggctg gcaaaagata ttgctgtcgt ttttccgccc
     4381 gaagttgtac acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca
     4441 gacaaattga cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca
     4501 gccacatttg acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc
     4561 agtcagatcg acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg
     4621 gcacttgaaa tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc
     4681 caggagggcg gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt
     4741 aaatgggatt atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac
     4801 ttggagcaat attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag
     4861 tctctctggc aaaaatgtac acaaaaactt attgccgcaa ttccacatat tccagaatgg
     4921 cggcaaccat taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc
     4981 cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact
     5041 gatgagcaca ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat
     5101 tactattgtg gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg
     5161 ccccgatttg ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat
     5221 catccgttcg cattgacact gcttatacgt gtagccgggc aaactaaacg ctgtcacgat
     5281 cggatgacga aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt
     5341 cttgggcaaa aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa
     5401 ccagcactgg cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa
     5461 tcatgccagc aacaactgac acagccctca aaccatgcct gcgccgatct actgcctgcc
     5521 atagtagtct ccccaccatg gctttcgaaa aagaaaaaag cgacgattcc ggtgctggag
     5581 ttagcgccat taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt
     5641 ttggcgaaat atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactgcc
     5701 aacctgttgg caagaatggg ttttcaacga cggatcgctg gtacatatat taaagctccc
     5761 gaagcggtag ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag
     5821 gtgtttcatt cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg
     5881 gagaaagcag taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac
     5941 gccatgttac attttggact caaagggtta cctgggttag taaactcact tgcacgctat
     6001 ccacaggaag ccttgcccat cacgaattac ttcgcagcga gtgagctggc acctgccgtc
     6061 gcccgcgcct tcaacaaact caaaactcta cgacaagacg cccgtagctg gctgctgaaa
     6121 tacccggaac acgccattac cggcctgctg cctgctgcgc tcggcaaagc cgatgaagct
     6181 caggataacg cccgcgctgc cttgcgtatg cttagcgaaa acggtcatca gccattactg
     6241 caagaaatcg cccggcgtta taaccagccg gaagtaaccg acgcggtgaa cgctctgctt
     6301 gcgctcgatc ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag
     6361 ccatcgctct ggacgcgccc ggtattaaaa gcaaacgccc aatcactgcc agatagcgcc
     6421 ctcctccacc tcggcgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta
     6481 ttgcaggtga aagatgcctg taccgccgac tcactggctg aatttgcctg ggatctgttt
     6541 accgcctggc agaccgctgg cgcgccgtcg agagagagtt gggcgttcac tgcgttaggc
     6601 gttctcggta acgatgacac cgcccgcaaa ctgacgccat taatccgcgc ctggcctggc
     6661 gaatctcagc ataaacgcgc caccgttggg ttggatattc tcgccgctat cggtagtgat
     6721 atagccctga tgcagcttaa cggcattgcc cagaaactga aattcaaagc attacaggag
     6781 cgggcgaaag agaaaattgc cgacattgcc gaaagtcgcg aactgacagt ggcggagctt
     6841 gaagatcggt tagcaccaga tctcggtctg gatgataacg gttcgttgct gctggatttc
     6901 ggcccacgcc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgacgct
     6961 tccggcagcc gtctgaaaga cctgcccaaa ccaaacaaaa gcgatgatga atcacaggcg
     7021 aacgatgctg ttaaccgcta caaactgctg aaaaaagatg cgcgtaccgt cgccgcccag
     7081 caggtagcaa ggctggaatc cgccatgtgc ctgcgtcgcc gctggtcgcc agaaaacttt
     7141 cagctcttcc tggttgaaca tccactggta cgccacttaa cccgccgtct gatttggggc
     7201 gtttatagca ccgaaaacca gctactgacg tgcttccgcg tggcggaaga taacagctac
     7261 agcaccgctg acgatgatct tttcaccctg ccggaaggcg atatctctgt tggcattcct
     7321 cacgttctgg aaatatcacc gacggatgct gccgcctttg gtcagctttt tgccgactac
     7381 aaactgctac caccgttccg tcagcttgac cgtaacagtt acgccctgac agaagccgag
     7441 cgcaacgcca gcgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg
     7501 gggctggcga ataaaggctg gataaagggc accccgcagg atggaggctg gatcggctgg
     7561 atgatcaaac ctctcggtcg ctggtcgtta atcatggaaa tcgacgaagg ttttgcggta
     7621 ggcatgtcgc cagccgaact gagtgccgag cagctcttaa gcaagctgtg gctatgggaa
     7681 ggcaaagcag aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta
     7741 ctcgatgcca tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga
     7801 aagagcatgg acaaggaatt accgtggctg gcggataacg ctcaactgga actgaaatat
     7861 aaaaaaggca aaacaccact cagccatcgc aattggccgg gcgaaccggt gcctgttatc
     7921 actgaaagca tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa
     7981 aacattgtct ggcgttatga aaatttttca ctggagtggc agtccgccat tacgcaggcc
     8041 atcaacttga tcggcgaaca caaaccctca gtcccggccc gcacaatggc ggtgctagcc
     8101 tgtatcgcgc aaaaggacag ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg
     8161 gaatatgcga ctgaggtggt gattgcacgc cagtttatta cacgatgtta tgagagtgat
     8221 cctctgctag tgacattgca gtatcaggac gaggattatg gctatggtta tcgctcagaa
     8281 acctataacg aattcgatct ccgactgcgt aagcatctct ctctggcaga ggaaagcagc
     8341 tggcagcgtt gcgccgacaa actcattgcc gcactgccag gaatacccaa aattcgccgt
     8401 ccttttattg cactactcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt
     8461 gaaagctccc ggtcaagtct ccattcaaag gagtggttaa aggtcattgc tactgataat
     8521 acggcggtaa aaaaactcga acgatgctgg ggcctggacg tatttagcga tcgagaagcc
     8581 agctacatgt cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa
     8641 caaggactgg cagccgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt
     8701 ctgctggtac aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa
     8761 aacaaaccca gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc
     8821 gccgcactgg cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc
     8881 atcgaagaca aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg
     8941 ttacaaacgc tgatagcatc gcagccacaa ctagcagaaa gggtgatgcc gtggttaagt
     9001 actcaagcca gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat
     9061 agcaccgata acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa
     9121 aaaatgacag ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg
     9181 caaccaggcg aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa
     9241 tctcttgcgg aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt
     9301 ggggatgatg tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc
     9361 aattcactca ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc
     9421 tggaactccc cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg
     9481 ttaattaaca tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg
     9541 aacctgtatt tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc
     9601 attgagaaaa agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat
     9661 gcgctaccag gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt
     9721 ttaaattttg gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg
     9781 gcgcagcgtg atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca
     9841 cttatccctc ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg
     9901 cgtttactgt acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag
     9961 cgtacagatg tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac
    10021 ccggcacgca ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga
    10081 cttatcacta ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg
    10141 cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag
    10201 ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc
    10261 cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca
    10321 cgggatctga cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc
    10381 agcggcctga acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat
    10441 atatcgcaac gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggtg
    10501 gtcgcagaaa atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg
    10561 ggccttgatg atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc
    10621 ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt
    10681 ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa
    10741 gggctaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc
    10801 ctacgtacca cccgacgctg gtcgctggca gattttcata ctctgtttgt taatcatccc
    10861 tttacccgcc tggttaccca gcgattcata tggggcgtgt atccggcaaa tgaaccgcgt
    10921 cgtttactca acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaggatgag
    10981 ccaattgacc tgcctgcgga tgctctgatt ggcattgccc acccgttaga aatgacagca
    11041 gaaatgcgca gtgaatttgc ccagcttttt gccgattacg aaattatacc gccacttcgc
    11101 cagttgacac ggcgcacggt gctgctcacg cctgacgagt cagccagtaa tagtttgaat
    11161 cgctgggaag gtaaatctgc taccgttggg caacttatgg gaatgcgata caaaggctgg
    11221 gagtcaggct atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag
    11281 ttttcacccg gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct
    11341 cttcgagtgt acagtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg
    11401 agtgaggcgt taagcgcacc tgacgtcatt ttccattaaa acacagcggg cagtgatgca
    11461 actgcccgtt atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg
    11521 gttgctattt acgattatga ggtccctgta cctgaagatc cgttttcctt cagacttgag
    11581 atccataaat gctctgaatt atttacaggt tccgtctatc gactggagcg attccggcta
    11641 cgtccaacat ttcatcaacg tgatcgagaa gatgctgacc cgctaataaa tgatgcgttg
    11701 atttatataa gagatgagtg tattgatgag cggaaattac gaggtgaatc acctgaaact
    11761 gtaatagcaa tttttaatcg tgaactacag aatatattca accaactaat agaatattag
    11821 tttattaaag ggggaagcgg ttttccccct tagttgcgtt tcaatttgcc cattacttca
    11881 acaaatcaat caaccgtaac agagacacta atagctccag tacggcaatg agcacggtta
    11941 acttttgtgt cagcgtgtcc atccgctctc cttattaagg agcgcaacac cagcacctta
    12001 cccttacact gcctgtcgat actggtttac gtttgttgag tcgccatgtt gcactccgga
    12061 tcagagcact acctgcggca ccacccgtcc ggtagccagg acttgaaatg ggttcgatcc
    12121 ggcgcaactt cccaacagtc aggattataa atctcattat cttaattgca acactgtcca
    12181 gaggtggtta taattcgttc gttgagatgc catgttgcac tatcagtaac ttaacatcaa
    12241 agtagtaaat aaaagaacct gcctcaccag caggtttttt ttattttctg cgatctactt
    12301 tccagatatt ttttgccaat agcccaccat aaatattcac atcccggtca aatggtgcgt
    12361 tgaatataaa atcgatgaat ttataaataa aaatgagtta tatgatgaat taataaaaac
    12421 agtaatcatt ttattaaagg gggaagcggt tttccccctt agatgcgttt caatttgccc
    12481 gttacttcaa caaatcaatc aaccgtaaca gagccactaa tagctccagt ac
//
LOCUS       DAMZII010000140        12483 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZII010000140 DAMZII010000000
VERSION     DAMZII010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.140"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            287..1162
                     /locus_tag="POA77_003893"
     CDS             287..1162
                     /locus_tag="POA77_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDI6367256.1"
                     /translation="MSDNNEVTHPFDVTNTETGKTYQLSPNSSKSVQPIALLRLSVFT
                     PVGTKEKRYRNFEVDASDELSSMELARSEGYDDIRITGLKLSMSTDFKCWLGCIMAFS
                     KYGFASDKITLSFNEFAKMCGISSTNINKRTRSRFQEALANLASVVISFRDSKTERFT
                     VTHLVQKAVIDPKKDTVELVGDPSMWELYRYDHKTLLSLQVLSVLAKKEAAQSLYIYF
                     EAMPAGTLFVSMKRLRERLLLTTPVRTQNQIIRKAMLELKSIGYLEYQEVKKGRDIQF
                     QIFKRSPKLALAKHS"
     gene            complement(1609..1953)
                     /locus_tag="POA77_003894"
     CDS             complement(1609..1953)
                     /locus_tag="POA77_003894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367257.1"
                     /translation="MQMLSNQRQLTLPHSFKCFPTKGNLHSPIRANAFRTKAPYTVPC
                     EPCFPTKSNFHRSIHTNAFFKRHFSAAIEANAFIQKALFCRHWSKCFYTKGTFLLSIE
                     TNAFHLLDIQFE"
     gene            2516..2869
                     /locus_tag="POA77_003895"
     CDS             2516..2869
                     /locus_tag="POA77_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDI6367258.1"
                     /translation="MELTLNTNVERGKTNAFAFLKSKHAKVPLFILLVLANCSFAYAG
                     SDDGAFGDIWAYMSEALTGAPGKIIACGMLFSVAYFGVVKPNLGLALVSALMMLVMAN
                     GEKIISSFLDAGIPL"
     gene            2888..3238
                     /locus_tag="POA77_003896"
     CDS             2888..3238
                     /locus_tag="POA77_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDI6367259.1"
                     /translation="MRGIFPLCGVFMTSQYEIPPHTYRFPYRINMPLLILFWDAKQLG
                     ITFVTIASGNIFDFFITSVVVAVVFWFAYKKAAEEGIRGKLKHKLWWYGFFPGKSVFS
                     SRYFTDPFIRNLYS"
     gene            3250..4035
                     /locus_tag="POA77_003897"
     CDS             3250..4035
                     /locus_tag="POA77_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDI6367260.1"
                     /translation="MKLLSRLKIPFIKNSSNGDFDNKDETSQKGGEEVKGSFLDSKAR
                     FSKEIEGSEIGITYSALIKRDEKLLRVNTVAMIIIGVLVVKNQFLTDPVTIVLPPNMT
                     EEVKVVGNKASESYKTQWALFFSTLIGNINPTNIGFVTTTILDALSPDLQAKTRESLQ
                     QQTNIMQARGVEQSFKPIDMYYDTKNDMVYVWGTKSTRLINVPDKTESSKWTFEWVLG
                     MKNGRPRIAYVNQYSGTPNIKKITINGKEQLATLDNPPPSIGN"
     gene            4038..5270
                     /locus_tag="POA77_003898"
     CDS             4038..5270
                     /locus_tag="POA77_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDI6367261.1"
                     /translation="MTSKKVFHCTAITAVILLATSNVVFAEDYQLPATVNNPVVMPVG
                     ADEFQNGVKNAIIKDTGTTTETVQATKPTTALPSLSPASSASPAVNEITGALSNNPTL
                     AGYQQQVKSGNFDSYGRPAGKEPQQNAQSTGSPSKADELYVEARNRYKEVQRVNVPPG
                     GNIVLPVSRGLQNRISTSFKNASVSTSTPADEASIFVNGGDVFISTNTDKPIGIMLSE
                     DSVPESTYNLTLVPLDVPGAMISVTTSLSPTMQAKRETSLDKQNYDEMLARSQSEELT
                     PSDPRQDDHKQRIIDLLTPVALGEVPSGFSLQEDRLSRIPSSEQSPCNFNMYAKLGQR
                     LVGSRELIDVVLVKNDKPYGQVVADQQCITEGVVASALFDKAFLQPGEETELYIVRDK
                     LFKERQTRVTTRPSLIKR"
     gene            5272..5712
                     /locus_tag="POA77_003899"
     CDS             5272..5712
                     /locus_tag="POA77_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367262.1"
                     /translation="MRKATFLTVISFGLVVFLGTSYYYQTKYIPKGTVNIFPDVHRQG
                     EIVDDAFEKSTIVISDPTLADKFVGSSTESVGRHEVNVIKELGTQNNGSKFVHDESDK
                     SGLTVGDKWPRAGEPYIVPQMTEYERNLKVKRFQQPKNGVNNGH"
     gene            5702..7060
                     /gene="trhB"
                     /locus_tag="POA77_003900"
     CDS             5702..7060
                     /gene="trhB"
                     /locus_tag="POA77_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhB"
                     /protein_id="HDI6367263.1"
                     /translation="MDIKKAWENKTVRISVIGAALMVLIVVISQSIFTTPVKKEKKTQ
                     KKDMQTGFLIDDSQMNKLSNEESQKTYNEMVRQNRIDQNAAKADRDKAEKAQQENKAQ
                     IASLASQVQQLTTQLTEMQTSRNGNRNLDAGGPRNNVNEQAPAKPYQLNPNAAVNGVS
                     SGYAPISPTRNSPMRTITQSSIKTNGTDGVIQVMPISENRIREGREVVAGSEKAPTRT
                     IRGDGTAPVDSKARHAARKDEMFLPATSIITGVLITGLEAPTSLSSKAEPMPVTMRIK
                     KDIIMPNNYTMDLRDCNLLGSAVGDLASQRAYIRATSISCVNSKGKAFDVIVEAYAVS
                     ENDGKNGIRGNLISRNGNAIAGSAFAGGLSALAGSLSPSKVSSLNIDPNSTAQYQSPN
                     IGALGALAGAGAAQGGLNRLVDYYTSIAEQQWPIVEISPGRPITFVVQKGATIPTNLT
                     SR"
     gene            7069..7581
                     /locus_tag="POA77_003901"
     CDS             7069..7581
                     /locus_tag="POA77_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6367264.1"
                     /translation="MENNNDNESPKKSESADSLNIDAAKPVGGIRTTKKVTLEIDIEK
                     TLKYLMVAGLAVLIVIYGYKGGRFVYDNFKAMSQPAYQIAVLDMQTLRKVFNKQNPVL
                     DSQQSKTNFENYFKALMKVYRERGYLVIDASLAVTIPENVEIVSYIDLGGSFGEVQSL
                     PGEPKESEIR"
     gene            7687..8439
                     /gene="htdT"
                     /locus_tag="POA77_003902"
     CDS             7687..8439
                     /gene="htdT"
                     /locus_tag="POA77_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase HtdT"
                     /protein_id="HDI6367265.1"
                     /translation="MEKAKEGGVSTKPLDGVLAKMKSYKPKDAIYIPTGGLYLFQDER
                     SQLMAVTTDGRYTITGGSVMDILQRKSVLSVEDIRKSFFINLDDAPFPLETVASIPLG
                     NPKLKRQAAIFITLDCDGCQDLIKKFYDEREKYRVDIVLIPSPGEPKQELRQLWCSKE
                     KGKVNDLDILRWLMGNKADIEKRLLTKEEAEACPAEPLVASLMLAGIYKLQGVPSVVR
                     QDGLAGNGIPKDFDYWLKQSVEPLLKNPFDTN"
     gene            8449..9399
                     /locus_tag="POA77_003903"
     CDS             8449..9399
                     /locus_tag="POA77_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HDI6367266.1"
                     /translation="MNIKKVIFSALVVGSSSYLAGCTIGSAESECPGIEKGVICKGPR
                     EVMELTNNRDDLSGLGEEEANSGKGKSAVNDSQYPAQLTPPGAVQYPKSSVLVNKPVT
                     YTTTEVKPVGQLPVMYDQTLKMGAPTSTIGPRPISGNPVNSNVRMTTSYNSNGSSVNP
                     FLHPSAEVVKKTTQVVTPAPAPRYVAPNSDINASKDLYSINNGQPVNPTLSSGQVQQY
                     RTQGYKQAVVAPEPLAVLQQGRVMRITFAPYTDDNDALNLPGFVYVNVKPQTWIAGKN
                     STSNPARIVPLEVQDAARENMQQQQRATKAVSSNGIVRQL"
     gene            9408..12089
                     /gene="trhC"
                     /locus_tag="POA77_003904"
     CDS             9408..12089
                     /gene="trhC"
                     /locus_tag="POA77_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer ATPase TrhC"
                     /protein_id="HDI6367267.1"
                     /translation="MRSANVYNKELSRHQLGGFIPVYDQIPGTHYFLLDGNRLGFMFI
                     CSPSPGVFDNQQDVLTELFKMDFPADTICQTSLTALPDLILHLSAWSAVRGGRMEGHD
                     KLKGDLLTAYQLDYYDRSLNEPLKPDHDKLMLRDFQVWISFSIPLKSALPSEIEKTRI
                     DALYSDLISKLNTVGLFPHKVGAENWLYCMDKLLHPGKTSRWSEGHVEASTMRRLNEQ
                     INVPGRKYTVTENHFSSTTQSNDISEHRYFKQLSVVKFPEFVNFGCMYELVVNWLNGR
                     KTIFSPFMITQTVHFADPLKLSRENVRYKAITNKQASIPTVLTFCPRLKDMDNDYMTI
                     TRELEDGARLLHSYLTFTVMGNSAVDVQSAADQLKSFYLESRVNVADDSYIVFPSFVS
                     SLPMCNDPKTILELDRFEVVSNTGAAHMTPIFGPWKGNTDRPVLNLVSREGQLLGLDI
                     FKTSASYNMVVGATSGAGKSFWVAYIINNYLGAGPRSNNLIHYRDTFEGFKNNSYDAF
                     DPDGAQIFVVDVGRSYQGISEQYTNSQFIDFGKKPDFTLNPFAFLTDVTVGERVFDEA
                     PVFNDDSNNHDDDKDKVAQTIMVLNQLKIMASEKGNIDDFQQSVMLQLISEEYNESRK
                     VGRTGSITGFARRCSNHEDKRIKDIGDQLGQWCEGGIYGNRFTENLPPINFDSRFIVL
                     ELEELKGTPHLQTVVLMSIIQAAQHAMFIKKDGRRRLFILDEAWEYIRPDNASGSSNH
                     SNQFFSSFLEAAWRRFRKTNCAGICITQSFEDYFTSSVGRALTANSPWKIIMKQEKES
                     IEAMKVNNYFSTTDAEYERMKNIRTVKRAFSEMLVRFENFQEICRLYVDRKMELCFTT
                     DSTDRGKLWEIQSRLDCSYGEAIEILYEQEVASKSAA"
BASE COUNT         3776 a         2444 c         2846 g         3417 t
ORIGIN      
        1 ggttaacaga cggaatttat ccacgttttc cacaagttag atcaattctt tagatcaaga
       61 gagatcaaga gagtacaaaa aagatcacag gttccgtaaa tgactgattt tattatggaa
      121 taagcatgat caggcattca ggtgcatgta aaaagcattt gattgaataa gaaaagcatt
      181 tacttgaatt gaaaaggcat ttagtttaat gcgagaaaag catttgcacg aataatctat
      241 tcacatctaa atgtatcaag ggataaacca tggtgggtaa atttaaatga gtgataacaa
      301 tgaggtaaca catccttttg atgtgaccaa cacggagaca gggaagactt atcaactgag
      361 tccaaactct tcgaaatcgg tccaacccat tgctttactg cgcttaagtg tttttacccc
      421 ggtgggaact aaagagaagc gatacagaaa ttttgaggtt gacgcttctg atgagttgtc
      481 gagcatggag ctggcccgtt cagaaggata cgatgacatt cggatcactg gtcttaagct
      541 ttcgatgtca accgatttta agtgttggct cggttgcata atggcgttca gcaaatatgg
      601 tttcgcttcc gataaaatca cattgtcatt caatgagttt gcaaaaatgt gtggtatcag
      661 ttctacaaac ataaacaagc gaactcgctc acgatttcag gaggcgctag caaacctagc
      721 ttccgttgtg atttctttcc gtgattcaaa aactgaacgt tttactgtca cgcaccttgt
      781 gcagaaagca gtgattgatc ctaagaagga caccgtagag ttggtgggcg atccctcaat
      841 gtgggagctt taccggtatg atcataaaac cttacttagc ttgcaggtgc tttcggttct
      901 cgctaaaaaa gaggcagcac aaagcctgta catatatttt gaggcgatgc ctgccggtac
      961 gttattcgta agcatgaagc gtttgcgtga gcgacttctg ttgacgactc ctgtacgaac
     1021 tcaaaaccag atcatcagga aggcgatgct ggagttaaaa tcaataggat atcttgaata
     1081 tcaagaggtt aagaaaggta gagatatcca gttccagatt tttaaaagaa gtccaaaact
     1141 tgctctggca aaacacagtt gatgctccgg gtatagtacg cacaaaagtt actttggatg
     1201 taatgaggag tatgggcatt catccaaatg ctttttaatg tttctccgga tttgaacacc
     1261 cttccctgtt tccaaacttc ctatcatttc tgatacccgg gttcttctgg tattcgttcg
     1321 aatgcttttc agaaaaaatc atgcttgcta gttgagacaa cgtaacctat tcattttgct
     1381 agtttaataa gactcattca tacatatgcc tttaactaca ctaccctcca tcgtgttgta
     1441 ttgggcttta aacgcaaatc cctatactcc ttgtgccact cggcgtattt acagttcctt
     1501 aacgcctgca aacgactggg atatgagcct ggtcgacggc attttatgaa tatgcaggcc
     1561 aatgaaggac ctagagaaat aacataacat tctgataata aatgaaaatt actcaaattg
     1621 gatatcaagg agatgaaaag catttgtttc aatggagagc aaaaaagtgc cttttgtata
     1681 aaagcatttg ctccaatggc ggcagaaaag tgccttttgt ataaaagcat ttgcttcaat
     1741 ggcggcagaa aagtgccttt tgaaaaaagc atttgtatga atgctgcggt gaaagttgct
     1801 tttggtcggg aagcagggct cgcaagggac agtgtaaggt gcctttgttc gaaaagcatt
     1861 tgcacgaata ggagaatgca agttgccttt ggtcggaaag catttgaatg aatggggaag
     1921 tgtaagttgc ctttggttgg aaagcatttg catgaatgag tccctgatag gtgtctttgt
     1981 ttgaaaagca tttgcatgaa tggattcaga agaagtgact tttacgataa gcatttgaat
     2041 gaatgggcac cgcagacgcg cattccccaa tcagcatttg aatgaataag ctccgcagtt
     2101 ttgctttcat cgaacagcat tcgtatgaat gtgctccgca gaggtggctt tcattgaaaa
     2161 gcatttgtat gaatggggga tacataatcc ttgtgggtga aaagcactcg catcaatggg
     2221 ggaaaagtga cttttggaat gtcatcctta ccgtgtcctt tgttaaatat ctggctggat
     2281 gaatgccata aaaatggtaa aggggcagac gatgcccctc ttccctattt ggcatgttaa
     2341 ttaaacgcac cttctttaat ccgattacat gcgggattct tttctgacat tcgatggcta
     2401 aaaaaacacc aagcgcaatt aagtcaaaaa aaaggtggtt aacagaaaaa tgaaagaaaa
     2461 ataaaaagta ttatgccaat gcgttttcgt gaattcaaat caacacggag taattatgga
     2521 actgacattg aatactaacg ttgaaagagg aaaaactaac gcttttgcat ttttgaagtc
     2581 caaacatgca aaagtgcctt tgtttattct cttggtttta gcaaattgca gttttgcgta
     2641 cgcgggttcc gatgatggag ccttcggcga tatctgggcc tacatgagcg aagccttgac
     2701 tggtgcgccg ggtaaaatca tcgcgtgcgg catgttgttc tcggtggctt atttcggcgt
     2761 tgtaaaacct aaccttggtt tggcgctggt atcagcatta atgatgctgg ttatggcaaa
     2821 cggtgaaaaa attatcagct cgttcctgga tgctggtatt cctctgtgat ttgttagaac
     2881 aataacaatg agggggatat tccccctctg tggagttttt atgacaagcc agtacgagat
     2941 accgccacat acatatcgct ttccatacag aataaatatg ccgttactaa ttctattctg
     3001 ggatgcaaag caattgggta taacattcgt gactatcgcg agcggtaata tttttgattt
     3061 ctttataact tcggtagttg tggcggtggt gttctggttt gcatataaga aagcagcaga
     3121 ggaagggatt agaggaaaac taaaacacaa actgtggtgg tacggctttt ttcctggaaa
     3181 atcagtgttt agtagtcgtt actttaccga tccatttatc agaaacctct attcttgatg
     3241 gagctaagaa tgaaacttct cagcaggtta aaaattccat tcattaaaaa ttccagtaat
     3301 ggcgattttg ataacaagga tgagacttcg caaaaaggag gtgaagaagt aaaaggaagt
     3361 tttctggatt caaaagcacg ctttagtaaa gaaattgaag gttctgaaat tggaattaca
     3421 tatagtgcat tgattaaacg cgacgaaaaa ctcctacgtg tgaatacagt tgcaatgatc
     3481 attattgggg ttctggttgt aaaaaatcaa tttctcactg atcctgtgac tatagtgcta
     3541 ccgccaaata tgacggaaga agtcaaagtt gttggaaata aagcctcaga atcatataaa
     3601 acgcagtggg ccttattctt tagtactttg attggaaaca taaacccaac aaacatcggt
     3661 tttgttacaa caactatatt ggatgcgctt tctccggatt tacaggcaaa gacccgggaa
     3721 tcactacagc agcaaaccaa tataatgcag gctcgtggtg tagagcagtc atttaagccg
     3781 atcgatatgt attacgatac caaaaatgac atggtctatg tctggggaac aaagtcaact
     3841 cgtttgatta atgttccgga caaaactgaa tcatccaaat ggacgtttga atgggtcctg
     3901 ggaatgaaaa atggacgacc gcgcatagcc tatgtgaacc agtattcagg tacaccaaat
     3961 attaagaaaa ttacaatcaa cggcaaagaa caactggcaa cactcgataa tccgccgccg
     4021 tcaataggta actaattatg acttcaaaaa aggtttttca ctgcaccgcg attacagcag
     4081 tgattttgct ggcaacttcg aatgtggtat ttgctgaaga ctatcagtta ccggcgacag
     4141 taaataatcc ggtagttatg cctgtcgggg ccgatgaatt tcagaacggt gtgaaaaatg
     4201 cgataattaa agacactggc actacgactg agaccgtaca agcaacaaaa ccgacaactg
     4261 ctctgccaag tctttcacct gcaagcagtg cttctcccgc agtcaatgaa ataaccgggg
     4321 cgttgtcaaa taaccctact ctagctggtt accagcaaca ggttaaatca ggaaattttg
     4381 atagttacgg aagaccagca gggaaagaac ctcaacaaaa tgcacaaagt accgggtccc
     4441 cttcaaaggc agatgaactt tatgttgaag cacgtaatcg ctataaagaa gtacaacgag
     4501 taaacgttcc gcctggtggc aatattgtac tcccggtttc tcgggggctg caaaacagaa
     4561 tttcgacttc atttaaaaat gcatctgtaa gtacttcaac gccagctgat gaagccagta
     4621 ttttcgttaa tggcggggat gtttttattt caacaaatac agataagcct ataggaatta
     4681 tgctttcaga agattcagtt cctgaatcta cttataacct gacactggtt ccactggatg
     4741 taccaggggc catgatttca gtaacaactt ctttgagccc gacaatgcag gcaaaacgag
     4801 aaacttctct tgataaacaa aattatgatg agatgctggc acgctcacaa tcagaagagt
     4861 tgactccttc agaccctcgg caggatgacc ataaacagcg gatcattgat ttgttgactc
     4921 cggttgcact gggtgaggtt ccttctggtt tcagtttaca agaagaccgc ttgtcacgca
     4981 taccaagctc agagcagtcc ccatgtaatt tcaatatgta cgccaagtta ggtcaaagac
     5041 tggtgggatc tcgggaattg atagatgtcg ttcttgttaa gaatgataag ccttacggac
     5101 aggtagtggc tgatcagcaa tgtataactg aaggcgtggt tgcaagcgct ctattcgata
     5161 aagcattcct tcagcccggg gaagagaccg aactttatat tgtgcgggat aaattattta
     5221 aagagcgtca gactcgtgtt actacgcgcc caagcctgat taaaagataa tatgagaaaa
     5281 gctacttttc ttacagtgat ctcgtttggt ttagttgtct ttttgggtac cagttattac
     5341 taccaaacta agtatatccc gaaaggcacc gtgaatatat ttcctgatgt ccaccgacag
     5401 ggtgagatcg ttgatgatgc ctttgagaaa agcacgattg ttataagcga cccaacgctt
     5461 gcggataaat ttgttggaag ttcaaccgag tctgtcggtc ggcatgaagt taatgtaata
     5521 aaagaattgg gcacgcaaaa taatggttcg aaatttgttc atgatgagtc tgataaatca
     5581 ggtttgacgg ttggagataa atggcctagg gctggtgaac cttatatcgt gcctcagatg
     5641 acagaatacg agcggaattt gaaagttaag cgattccagc aaccaaagaa tggagttaat
     5701 aatggacatt aaaaaggcct gggaaaataa aactgtcaga atctcagtaa ttggtgctgc
     5761 attgatggta ttgattgttg tcatcagtca atcaatcttt accacaccag ttaaaaaaga
     5821 gaagaaaacc cagaaaaaag atatgcaaac tggattcctt attgatgatt cacaaatgaa
     5881 caagctgagt aatgaggaaa gccagaagac ttataacgaa atggtaaggc agaaccgtat
     5941 tgaccagaac gctgccaagg ctgaccgtga caaagctgaa aaggctcagc aagaaaacaa
     6001 agcacagata gccagtcttg cttcgcaagt tcagcaactc actacgcagt tgactgaaat
     6061 gcagacctca agaaatggta atcgcaatct cgatgcaggc ggaccacgca acaacgtcaa
     6121 tgaacaagcc ccagcaaagc cataccagct taatccaaat gcagcggtga acggggttag
     6181 ttctggatac gcacctatat ctccgacccg aaacagtcca atgcggacaa taacacagag
     6241 ttcaattaag acaaacggca ctgatggtgt cattcaggtg atgccgatat cagagaaccg
     6301 gatcagagag gggcgcgagg ttgttgcggg aagtgaaaag gccccaacgc gaactattcg
     6361 cggcgatggg actgcacctg ttgatagtaa agctcgtcat gcggcccgta aggatgaaat
     6421 gtttcttcca gcgacgtcaa ttattactgg ggtgctaatt accgggctgg aagctccaac
     6481 gagtttgtcc tctaaagcag aaccgatgcc ggttaccatg cgcattaaaa aagacatcat
     6541 catgcctaac aattacacga tggatttgcg tgattgtaat ttattgggtt ctgctgtggg
     6601 ggatctggca tctcagcgtg cgtacattag ggcaacctcc atatcgtgtg tcaactcgaa
     6661 aggaaaagca ttcgacgtaa tagtggaagc ttatgcggtt agtgaaaatg acgggaaaaa
     6721 tggcattcgt gggaatctga taagcagaaa tggaaatgcg attgcaggtt ctgcgtttgc
     6781 aggagggctg tcagcattgg cggggagtct gagtccatca aaggtgtctt cgcttaatat
     6841 agatccaaac tctacggcac aataccaatc gccaaacata ggtgcattag gagcgctggc
     6901 cggagccggt gcagctcagg gggggctaaa ccgtcttgtg gactactata cgtcaatagc
     6961 tgagcagcag tggccaattg tagaaattag cccagggcgc ccaattactt ttgtcgttca
     7021 aaaaggggcg acaataccta caaacctgac aagccgctga gaataattat ggaaaataac
     7081 aacgacaatg aatcaccaaa aaagagtgag tctgctgata gcttaaatat cgacgcggct
     7141 aaaccggttg ggggtatacg tactaccaaa aaagtaactt tagagatcga tatcgagaaa
     7201 actcttaagt atctgatggt cgcaggcctg gcagttctta tcgtgatcta tggatacaag
     7261 ggtggacgtt ttgtatatga caacttcaaa gcaatgtcgc aaccggccta ccagattgcc
     7321 gtgctggata tgcaaacttt aagaaaagta tttaataagc aaaatccggt gttggattcg
     7381 cagcaatcca aaactaactt tgaaaattat tttaaggcat taatgaaggt ttaccgtgaa
     7441 cgtggctatt tggttattga tgcatccttg gcagtaacta ttccagagaa tgttgaaata
     7501 gtctcttata tcgaccttgg tggaagtttt ggtgaggtac aatctcttcc aggcgaacct
     7561 aaagaaagtg agattcgata attgaaaaag ttacttttgt ctctgctgat ttccattacc
     7621 tcagcgtgcc cgccgcttac tctggctgtt gaaaatgtat taaacaatcg tgatgccgct
     7681 gcgttaatgg aaaaggcaaa agaaggtgga gtatcaacga aacctttaga cggtgtattg
     7741 gcgaaaatga aaagctataa gcctaaagat gctatttaca tccctactgg cggtctttat
     7801 ttgttccagg atgaacgttc tcagttgatg gcggtgacca ctgacggacg gtatacaatc
     7861 acggggggga gcgtcatgga cattcttcag agaaagtcag tgttatcagt agaggatata
     7921 cgaaagtcgt tctttattaa tttagacgac gcccctttcc ctcttgaaac tgtggcatcg
     7981 atacctttag gaaacccaaa actcaaacgt caagcggcaa tatttattac gcttgattgt
     8041 gacggttgtc aggatttaat aaagaaattc tatgatgaac gcgaaaagta tcgggtcgat
     8101 atagttttaa tcccctcccc cggcgagcca aagcaggaat tgcgtcaatt atggtgcagt
     8161 aaagaaaaag gcaaagtgaa tgatcttgat atcttgcgtt ggcttatggg taataaggcc
     8221 gatatagaaa aacgactcct gacgaaagaa gaggcagaag catgtccggc tgaaccgtta
     8281 gtcgcgtcat taatgctggc tggaatttat aaattacagg gagtcccctc agttgtaaga
     8341 caggatggac tcgctggtaa tggcataccg aaagattttg attactggtt aaaacaaagt
     8401 gtcgaaccct tactcaaaaa cccatttgat actaattaag gtaatgaaat gaacattaaa
     8461 aaagttattt tcagcgctct ggttgttggt tcatcttcat acctggcggg ttgtactatt
     8521 ggcagcgcag aatctgaatg cccgggaatt gaaaaaggag ttatttgtaa aggcccgcgt
     8581 gaagttatgg aactgacgaa caatcgtgat gatctctccg gccttgggga ggaagaggct
     8641 aattcgggaa aggggaaatc ggctgtcaat gacagccagt accctgccca actaacgccg
     8701 cctggcgcag tgcagtaccc caagtcgtct gtacttgtta ataagccagt gacatacaca
     8761 acgacggaag taaaaccggt tggacagctc ccggtgatgt atgatcagac attaaaaatg
     8821 ggggcgccaa cttcaacaat tggaccacgg ccaatcagcg ggaatcctgt taattctaat
     8881 gtacggatga caacgagtta caactcaaat gggtcatccg ttaacccctt tttgcatccg
     8941 tctgctgaag tcgtgaaaaa aacaactcag gttgttactc ccgcgcctgc tccgcgctat
     9001 gtagcaccaa attctgatat caatgcaagt aaagaccttt attccattaa caatggacag
     9061 ccagtaaacc ctacgctcag ctctgggcag gtacagcaat accgtacaca aggttacaaa
     9121 caggcagtcg ttgcaccaga gcctcttgct gtgcttcagc aaggccgcgt catgagaatc
     9181 acctttgcac catatacgga tgataatgac gcgctaaatc tgcctggctt tgtttatgtg
     9241 aatgtcaaac cgcaaacttg gattgcgggc aaaaattcaa catcaaatcc ggcacgaatt
     9301 gttcctttgg aagtccagga tgcggctcgt gaaaatatgc aacagcaaca gcgagcaacg
     9361 aaagcagttt cgtcaaacgg tattgtgaga caactctgag gtcacgaatg agatcagcta
     9421 acgtctataa taaagaatta agccgtcatc agttaggtgg atttattccc gtgtatgatc
     9481 agattccggg tacacattat tttctacttg acggaaaccg actcggtttt atgtttatct
     9541 gtagtccgtc gcccggtgtc tttgataatc agcaggatgt gttaactgaa ttatttaaaa
     9601 tggacttccc agctgacacc atctgtcaga cctcattaac tgcgctgcca gacttgatct
     9661 tgcacttaag tgcctggtct gccgttcgtg gtggtcgaat ggaaggtcat gataagctta
     9721 agggggattt actcactgca tatcaactgg attattatga ccgaagcctg aatgaacctt
     9781 tgaagccgga ccacgataaa ttgatgcttc gagattttca ggtctggatt tccttttcaa
     9841 ttccattgaa gtcagcactt ccttctgaaa tagaaaagac tcgcattgat gcgctttact
     9901 ctgacttaat cagtaagttg aatacagtag gccttttccc acacaaagtt ggtgctgaaa
     9961 attggcttta ttgcatggat aagttattgc atccgggaaa aacatcgcgt tggtctgaag
    10021 gtcatgttga agctagcacc atgagacgcc taaacgagca gatcaatgta ccgggtcgaa
    10081 aatatacagt gacagagaat catttctcat ctacaacgca gagcaatgat atttctgagc
    10141 atcgttattt caaacaatta tcagtagtga agtttccaga atttgtaaac tttggctgca
    10201 tgtatgaact tgtagttaat tggttgaatg gacgtaaaac gatctttagc cctttcatga
    10261 taacccagac tgtccatttc gccgatcctt taaaattatc aagagaaaac gtcagatata
    10321 aggcaattac taacaaacaa gccagtattc caacagtttt gacgttttgt ccaagattaa
    10381 aggatatgga taatgattac atgacgataa cacgcgaact ggaagatggg gctcgcctcc
    10441 tacacagtta tctgacattt actgtaatgg ggaattcagc agttgatgtt cagtctgccg
    10501 cagaccagtt aaaatcattt tatctggaaa gtcgtgttaa tgttgctgat gactcgtaca
    10561 tagtatttcc ctcatttgtt tcaagcctgc cgatgtgtaa tgacccaaag acaatactag
    10621 aactggatcg ttttgaggtc gtgagtaata ctggtgctgc gcacatgacc cccatatttg
    10681 gtccttggaa aggtaatacg gacagacccg tacttaacct tgtctcacga gaaggacagc
    10741 tgttggggct ggatatcttc aagacttctg ccagctataa catggtcgtt ggtgcaacgt
    10801 ctggtgctgg taaatcattc tgggtcgcat acatcattaa taattatcta ggcgcaggtc
    10861 cgcgttctaa taatctgatc cattatcgag atacttttga gggattcaaa aacaattctt
    10921 atgatgcatt tgaccctgat ggagcccaga ttttcgtagt tgatgtcgga cgctcatacc
    10981 aaggtatttc ggaacaatat actaatagtc agtttataga ctttgggaaa aagcctgatt
    11041 ttacgctaaa tccattcgca tttttgactg atgtgactgt tggagagcgt gtttttgatg
    11101 aagcacctgt ctttaatgac gattcaaata atcatgatga tgataaggat aaagtagccc
    11161 agaccattat ggttctaaac cagttaaaaa taatggcatc tgaaaaagga aatattgacg
    11221 attttcaaca atcggtcatg ctgcaactga ttagtgaaga atataacgaa tcacgcaaag
    11281 ttggacgtac aggctctatt actggatttg cgcgacgctg ttctaaccat gaagataagc
    11341 gtattaaaga tatcggtgac cagttggggc aatggtgtga aggagggatt tatgggaatc
    11401 gctttacaga aaatctcccg ccaataaatt ttgatagccg gtttattgtt ctggaacttg
    11461 aagaattgaa ggggacccca caccttcaga ctgtcgttct gatgtcgatt attcaggccg
    11521 ctcagcatgc aatgtttatc aagaaagacg gacgccgccg gttgttcata cttgatgagg
    11581 catgggagta tatccgtccg gacaatgctt caggttccag taatcattca aatcaatttt
    11641 tctcctcttt ccttgaggcc gcttggcgcc gattcaggaa aacgaactgt gccggtattt
    11701 gtattactca atcctttgaa gattacttca cctcatcggt tggtcgcgca ctaacggcta
    11761 actcgccgtg gaagatcatc atgaagcagg aaaaggaaag tattgaagcc atgaaggtga
    11821 acaactactt ctccacaact gatgcggaat atgagcgtat gaaaaatatt cgaacggtaa
    11881 agcgtgcatt ttccgagatg ctcgtacgat tcgaaaattt ccaggagata tgtcgcctct
    11941 atgtagatag aaaaatggag ctttgcttta cgactgacag caccgatcgt ggaaaactat
    12001 gggaaatcca aagccgactt gattgctctt acggcgaagc aattgagatt ctgtacgaac
    12061 aggaagttgc gagcaaatca gctgcctgac gttttaagtt actggttacc tcattttgtt
    12121 ttgactgagg tatccagtac gtctttgaag taaatagttg ttagctgtca ttgcctgccc
    12181 tcccccgaaa taatagcccg ggttaagaat atctagctcg gattaagtcc tctcaaaccg
    12241 tcatacatga aattcgatgc ctttgtcatt cttcttttga aggtgaatga atatgatgaa
    12301 atttactcca gaattattct ccctaccagg cttttgctgt gaagttaatc agagcaggta
    12361 ttatgttttg ggtacaagaa ccaaccaagg gggtgtggtt tttgtatggc agatacaaac
    12421 atcattttta cggttataat aaacaaccat tatatagatg aatttatcgt tttttctttt
    12481 aag
//
LOCUS       DAMZII010000141        12432 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZII010000141 DAMZII010000000
VERSION     DAMZII010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.141"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            850..1221
                     /locus_tag="POA77_003905"
     CDS             850..1221
                     /locus_tag="POA77_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367268.1"
                     /translation="MKTSERYPVTQGDVIVNYASSLFDRTEVTAPSLPADVFCGDILD
                     SKTFALFDSAAADAGKPIINLSNMSADGKPKTLVIVDRHVVITDFALRAKDAASKKAA
                     LEALTASGDIRLAINADYFKA"
     gene            1234..2253
                     /locus_tag="POA77_003906"
     CDS             1234..2253
                     /locus_tag="POA77_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDI6367269.1"
                     /translation="MAIDFKQFEKLDTVAALKQVPAGNYLISDLGIFSGVNSTTPNAQ
                     VLDIVETQVSELEQVARYGTEVNSIKMDKPLLRDQELPHYATEADVKTADWQGLISPA
                     NPDQVAAISEVIASKAIRMRNHHLETVESTLARALFKQEAKAAKTDDGDIDFRAAMGA
                     DPLSFDLDGSAGANVYAQLAKIRRMLVKEYGASRSYLDRFFCFCSPDLYDVLASHPEV
                     TSLITNKVAEAAKGALIPVNTAGYDSFSIGNITFILADDDRYEIEEGSGLFVPKFNGA
                     DRNPFKLVHGPASRNQDIAAKGEISPFYQKVMTDRYGMVTIHQEASFIPLNLRPNYTL
                     NVKLK"
     gene            2346..2537
                     /locus_tag="POA77_003907"
     CDS             2346..2537
                     /locus_tag="POA77_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367270.1"
                     /translation="MKVSINIPGAGQVYVSGAAEVINRKALCKELGICNITLCRLEAQ
                     HGFEYALSVLLSKKRKLLN"
     gene            complement(2573..4018)
                     /locus_tag="POA77_003908"
     CDS             complement(2573..4018)
                     /locus_tag="POA77_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6367271.1"
                     /translation="MFIKKLKIENFKGYHGEDNELEFNIPDGETEGSGLNIFVGENNA
                     GKSTVFEIIDFIKDGTKKNIEDLICKSDPANIAEKLSAELTFTGNLSEVIAAHVQANK
                     VAAFTSHVFEDEGVNYFRVKRCSEVGEKKILFWNGNEFVNPSGIDAPFKKFYDNNFIW
                     ADTNPSNEAKFGSTTICGSLLKEIALGHIETDEYRAFSEQYHRVFNHPESELRQKISL
                     VENQVREVFSSQFGMADISFIFEELQVENFFKTISIMIDDGVNVPMGEKGHGMQRALA
                     LSLLQVYAEITCNAHGAGISKPFYLFIDEPEICLHPSGQQKLLEALMIISKSRQVFVT
                     THSPFLLSSPYLKNIGLFVFKKENNKNVVERADTSPWFPWSPSWGEINFKAYNLATVE
                     LHNELYGYLQEKNSCPSEKTMEQWLNSKGIAFDKVWVKEFKGAAQPPADTTLQTFIRN
                     HIHHPENVTMQSHRYTQQELLTSIQQMVGLL"
     gene            complement(4132..5490)
                     /locus_tag="POA77_003909"
     CDS             complement(4132..5490)
                     /locus_tag="POA77_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6367272.1"
                     /translation="MGHRTIRHTITRNGIYYVRFRLPGNKYFRKSLETDSHTQAQLLM
                     SFASPVIPLVQRGTIQPDHFGKRLSEYGNSLKQQNEQWLAHQFLNDEQCNIQPIVAQE
                     YREIVTPSGDEEEQIEAQPKEVLTLAGAWNMYKKEKAQNWTKAISQANERFIEVMLIV
                     LGSSTDVTTITKQDIKQVMEVVENLPKRVVQPYRSMTVQQLIECDDVPPEDLVGAESI
                     HKHLKIYKSLFKTFLTDNKDILEKSPTDGVVAAPSKARFGAYSAAEMRKFVEWALKQP
                     DGWQKWITLLLAYTGARRGEIAKLEKSQIKFDEDSQRYYLLIAEGGQGKTENATRQVA
                     IHPKLIEWGFLDFVNRQWKEKIFSPVSGKNMPKIGKVLADVRDQLGIPYLDDYGQRRL
                     VHSFRHTMISTCLAGWVGNLAHLQQVVGHEKSGAGITRRYLHIFPLKTVCYVIDGLCW
                     NG"
     regulatory      complement(5557..5653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5745..6017)
                     /gene="rcnR"
                     /locus_tag="POA77_003910"
     CDS             complement(5745..6017)
                     /gene="rcnR"
                     /locus_tag="POA77_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDI6367273.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            6138..6962
                     /gene="rcnA"
                     /locus_tag="POA77_003911"
     CDS             6138..6962
                     /gene="rcnA"
                     /locus_tag="POA77_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDI6367274.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHDEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            7181..7519
                     /gene="rcnB"
                     /locus_tag="POA77_003912"
     CDS             7181..7519
                     /gene="rcnB"
                     /locus_tag="POA77_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDI6367275.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(7601..8635)
                     /locus_tag="POA77_003913"
     CDS             complement(7601..8635)
                     /locus_tag="POA77_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDI6367276.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(8651..11131)
                     /locus_tag="POA77_003914"
     CDS             complement(8651..11131)
                     /locus_tag="POA77_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6367277.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(11147..11821)
                     /locus_tag="POA77_003915"
     CDS             complement(11147..11821)
                     /locus_tag="POA77_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6367278.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(11901..12431)
                     /locus_tag="POA77_003916"
     CDS             complement(11901..12431)
                     /locus_tag="POA77_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367279.1"
                     /translation="MIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGD
                     SVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGN
                     DKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSV
                     QSGDVLSSLVMRVAQD"
BASE COUNT         3293 a         3034 c         2568 g         3537 t
ORIGIN      
        1 cattcgtgtt aaaatattat acatggtgtg ggatgtggtg agcatatcga aattaccgtt
       61 ttgcagcttg ccacccgcac cattacagaa cgtaggtttt agccaacctg ttggggtctg
      121 tagagagccg ggcggcttct ccccgcccgg ccctcaccca aagcgttctt tcaactccac
      181 ttgccagaat gctttgtgtg cggtcctctc ctcaagttcc gccagttgat gacattccta
      241 cgattcaaaa tgcctctaat catagggctt gcgccacagg ccctatttta aagcgtcgct
      301 aactgcggct cttcagaata caagcggaat ctccttcgat tgtattgacc ctttaggggt
      361 ccacagttgg gccgggctgg ttacctggtc tcaacgataa actgttgatt tgtgtcgatg
      421 gtttccgggg cgttggttgc gtcccgtttt cctctgtggc ttctttgctg aaccatgaaa
      481 gcaaatcatg taaggctctg aggctacaca ggaaaacaag tgatcagaca gtaagttaca
      541 ttgtcttctc ccggtctctc ctaccgggtt cttttttgtg gttatttgcc catcggctgg
      601 cggttgctcc cgtgacgctg gcgggtgagt gaccacaaaa aagaggttta cacctccccc
      661 caccctagag ggtgagagag agccagatta gaaagggcgg cgcggatgcc gccctcttct
      721 attcacccca cggcctccca tcttccaaca attcggattg tcctctgtgg cggtccttat
      781 atccggcgtt gcaccggatc accctgtgga ctctctccac gtctaaaacc ctccataagg
      841 aaaaccttta tgaaaacttc cgagcgctac ccggtaacgc aaggtgacgt aatcgtgaat
      901 tacgcctcca gcctgtttga ccgcaccgaa gtcaccgccc caagcctccc ggctgacgtc
      961 ttctgcggtg acatcctgga cagcaaaact tttgcactgt tcgactccgc cgccgctgac
     1021 gctggcaagc cgatcatcaa cctttccaat atgtccgccg atggcaagcc taagactctg
     1081 gttatcgtgg atcgccatgt tgtgattacc gattttgccc tacgtgctaa agacgccgcg
     1141 agcaaaaaag ccgcactgga agcgctgaca gcctccggtg atattcgcct ggctatcaac
     1201 gccgattact tcaaagcata aggaaatcac acaatggcta ttgattttaa acagtttgaa
     1261 aagctggaca ccgtagcggc acttaaacaa gtgccagcgg gtaattacct gatctctgat
     1321 ctgggtatct tctccggtgt taactccacc actccaaacg ctcaagttct ggacatcgta
     1381 gagactcaag tctctgaact ggaacaggta gcccgctacg gcaccgaagt taactccatc
     1441 aaaatggata agcctctgtt gcgtgaccag gagctcccac actacgccac cgaagcggac
     1501 gtaaaaaccg ctgattggca aggtctgatc agcccggcta acccggatca ggttgcggct
     1561 atttctgaag tgatcgcgtc taaagctatc cgtatgcgta accatcacct ggaaacggtt
     1621 gaaagtacgc tggcccgcgc cctgttcaaa caggaagcga aagcggctaa gactgacgac
     1681 ggtgatatcg actttcgcgc ggcgatgggt gccgatccgc tgagtttcga cctggacggt
     1741 tcagcaggcg ctaacgtgta tgcacagctt gcgaagattc gccgcatgtt ggttaaagag
     1801 tacggcgcga gccgctccta tctggaccgc ttcttctgct tctgctcccc ggatctgtat
     1861 gacgtactgg cgagccaccc ggaagtaacc agccttatta ccaacaaagt ggcggaagct
     1921 gcaaaaggcg ctctgatccc ggttaacact gcgggttatg actccttcag cattggcaac
     1981 attactttca ttctggcgga cgatgaccgc tacgaaatcg aagaaggttc cggcctgttc
     2041 gttcctaagt ttaacggcgc tgaccgtaac ccgttcaaac tggtacacgg cccggcttcc
     2101 cgtaatcagg acatcgccgc gaaaggcgag atcagcccgt tctaccagaa agtaatgact
     2161 gaccgttacg gcatggtgac cattcaccag gaggcgagct tcatccctct gaacctgcgc
     2221 ccgaactaca cgctgaacgt caagctgaag taatgcaaaa tgccccccgg tcgcgccctt
     2281 tgggtatcgg ctgggggtta tctcttccgg ttatattcat ttttactttt acactgaggt
     2341 aacacatgaa agtttcaatc aatatccccg gcgctggtca agtctatgta tcaggcgcgg
     2401 cggaagtaat taatcgaaag gcgctgtgta aggagttggg gatctgtaat atcaccctat
     2461 gccgtctaga ggcgcaacac ggatttgaat atgctttaag cgtactatta tctaaaaagc
     2521 gcaagttatt aaattaatat taaaaggcga gccaaacggc tcgccttttt ctttatagaa
     2581 gcccaaccat ttgttggatt gaagttagta attcttgttg tgtatagcga tgagattgca
     2641 ttgtaacatt ctctgggtgg tgtatatgat tacggatgaa tgtttgtaat gtagtatccg
     2701 ctggcggttg tgccgcacct ttaaattctt taacccatac tttatcaaac gcaattccct
     2761 ttgaatttag ccattgctcc attgtttttt cgcttggaca ggagtttttc tcctgcaaat
     2821 aaccatataa ttcattatgc aattcaacgg ttgctaaatt gtatgctttg aaattaatct
     2881 cgccccacga tggagaccaa gggaaccatg gagaagtatc agccctttcc acaacatttt
     2941 tattattctc ttttttgaaa acaaacagac cgatattttt aaggtaaggg gaggacaaaa
     3001 ggaaaggaga gtgcgttgta acaaaaacct gtctactttt ggagataatc attaaagctt
     3061 ctaaaagctt ttgctgccct gaaggatgaa gacatatttc aggttcgtca atgaaaagat
     3121 aaaatggttt actaatcccc gcaccatgcg cattacacgt tatctcagcg tacacctgaa
     3181 gtaatgatag ggctagtgcc ctttgcatac catgcccctt ttctcccatt gggacattca
     3241 ccccatcatc aatcatgatg gaaatagttt tgaagaaatt ctctacctgc aactcttcaa
     3301 aaatgaaaga aatatctgcc ataccaaatt gagatgagaa aacctctcta acttgatttt
     3361 cgactaatga tattttctgt ctcaactctg attcagggtg attgaatacg cgatggtatt
     3421 gctcagaaaa cgctctatac tcatccgttt caatatggcc taaagcaatt tcttttagaa
     3481 gcgaaccaca tatagttgta gagccaaact ttgcttcatt gcttgggttt gtatcagccc
     3541 aaatgaagtt attatcataa aacttcttaa atggggcatc tataccactt gggttaacaa
     3601 attcatttcc gttccaaaat aatattttct tctcccctac ctcactacac ctcttcactc
     3661 tgaaataatt aacaccctcg tcctcaaaaa catgcgaggt gaatgctgca actttattag
     3721 cttgtacatg agcggcaatt acttctgata agtttccagt gaaggttagc tcggctgaaa
     3781 gcttctcagc aatattagcc ggatctgatt tgcatataag atcctcaatg ttctttttgg
     3841 ttccatcctt tataaaatct ataatttcaa atacagtgga ttttccggcg ttgttttcac
     3901 ccacaaatat attaagtcca ctaccttccg tttctccatc agggatattg aattccaact
     3961 cattatcttc cccgtgatac cctttaaaat tttctatctt cagtttttta ataaacatca
     4021 ttaatccttt atgctagctg tgtatagaaa aataacctta cgtagaacac cttattttta
     4081 catgatttat atcactgaaa attcagtaaa actatgacat cacccaaatt attagccatt
     4141 ccaacacaat ccatcaatga cgtaacacac agttttcaga ggaaaaatgt gtaagtatct
     4201 tctagtgatc cctgccccgc ttttctcatg ccctacaacc tgctgcaagt gcgctaagtt
     4261 gccaacccag ccagctaaac aggttgaaat catggtatgt cggaagctat gcaccaatcg
     4321 ccgctgccca taatcatcaa gataaggaat gccaagctga tctcgcacat ctgctaaaac
     4381 tttgccgatc ttcggcatgt ttttgccaga cacaggggag aaaattttct ctttccattg
     4441 acggttaacg aaatcaagga aaccccactc gatcagtttg ggatgtatcg ccacctgtct
     4501 tgtagcgttc tcggttttac cctgtcctcc ttcagcgatc aggagatagt accgctggct
     4561 atcttcatca aatttgatct gtgatttctc cagcttggcg atctcccctc ttctggctcc
     4621 cgtgtacgcc aacaaaagag tgatccactt ctgccaaccg tcaggctgct tgagcgccca
     4681 ctccacaaac tttctcattt ctgctgcact gtatgctcca aacctcgctt tcgacggtgc
     4741 ggcgactact ccatcagtcg gagacttctc cagaatatct ttattgtcag tgaggaaggt
     4801 tttaaagagc gatttgtaaa tcttcaaatg cttgtggata gactcagcgc caaccaaatc
     4861 ttcgggcggg acatcatcac actcgattag ctgctgaaca gtcatcgacc gataaggctg
     4921 cacaacacgc ttaggcaagt tttcaacaac ctccattacc tgtttaatgt cttgcttggt
     4981 aatcgtggtt acatcagttg atgaacccaa gacaatcaac atcacctcaa taaagcgctc
     5041 attggcttga gagatagcct tcgtccagtt ttgggctttc tcctttttgt acatgttcca
     5101 ggcgccagcc agcgttagta cttcctttgg ttgtgcttct atttgttctt cctcatctcc
     5161 cgaaggagtg acaatctcgc ggtattcctg agcaactatc ggctgaatat tgcactgctc
     5221 atcattcaga aattggtgcg ccagccattg ttcattctgc tgcttcaggc tattgccata
     5281 ttcagaaaga cgcttaccga agtgatccgg ttggatggtt ccacgttgaa ccagaggaat
     5341 tacaggagaa gcaaaagaca tgagcaactg agcctgggtg tggctgtcag tctccagaga
     5401 cttgcgaaaa tatttattac cgggcaagcg gaaacggacg taataaatcc cgttgcgtgt
     5461 gatggtgtga cgtatggttc tgtggcccat tctgaaagcc tcattgagta atgaactatc
     5521 aaaacaggac gttaaagcta gaaatttctt ttgattttca atggataaca gccagactac
     5581 gctggcatta tccagatcag gtgatacggg tatttctcag ccttcacgca gaagggcacc
     5641 ccgagtcgtt tggttgcgat gataaatcgc agaggaggat ggtaatgtcc agcgcacgcg
     5701 ttgtaaacga ggaaaaacgg cagttttaca atcgcgttaa taaattattt gatatatgaa
     5761 tccagcacct tcagaacgac atccagatct tcttcacgtt ttagctcatc cccctggtga
     5821 acgatgtgtt ccgtcagatg acctttaatc acttcccgca tcagaccgtt taccgcgcca
     5881 cggatagcag caatctgttg taaaactgca gcgcattcgt gcggctcgtc gagcattttc
     5941 ttgagcgcca cgacctgacc ctgaatctta ctggcacgcg ctttcagttt ctgtttatca
     6001 cggattgtat gagacatggc aacaccttgt taacaagaac atgaaaaatc atagcactat
     6061 taatctactg gggggtagta tcaggtactg ggggggagta gaatcagatt gccgaattaa
     6121 tactaagaat tattatcatg accgaattta caactcttct tcagcaagga aacgcctggt
     6181 tcttcatccc cagcgccatc ttacttggtg cgcttcatgg cctggaacca gggcactcaa
     6241 aaacgatgat ggcggcgttt atcatcgcca tcaaaggcac cattaaacag gcggtgatgc
     6301 tcggactggc agcaactatt tcgcataccg cagtggtctg gttgattgcc tttggcggga
     6361 tggtggtcag caagcgcttt actgctcaat cagcagaacc gtggctccag ttgatttccg
     6421 cagtgatcat tattagcacc gcgttctgga tgttctggcg tacctggcac ggcgagcgca
     6481 actggctgga gaatatgcac gagcatgatc atgagcatca tcatcacgat cacgaagatc
     6541 accacgacca tggacatcat caccatcacg aacatgacga gtatcaggat gctcatgcac
     6601 gagcccatgc taatgacatt aaacgacgct ttgatggtag agaggtcacc aactggcaaa
     6661 ttttgctatt tggcttaacc ggtggcctta tcccctgccc ggcagcaatt accgtgctgt
     6721 tgatttgcat tcagttgaaa gccctgacac tgggcgccac actggtcgtc agtttcagca
     6781 ttggcctggc gttaacgctt gtcaccgtag gcgttggcgc agcaatcagc gttcagcagg
     6841 tcgcaaaacg ctggagcgga tttaacactc tcgctaaacg cgccccctat ttttccagtc
     6901 tgttgattgg cttagttggt gtgtatatgg gcgtacatgg cttcatgggc ataatgcgat
     6961 aactcaatcc tttaggcttg catgttatct tcagcccagg atgattctta aatcagctat
     7021 ttccgctgac tctctgctcg ctaaggatgc ttttagggca tcctttcatt tacacttttt
     7081 acgaaatcat gggatcacta acaaaatatc gcttgtcagt tatattgtat ggcaggaaag
     7141 atatgcgact gatattacag atccccaaag tggagagttt atgaccatta aaaataagat
     7201 gttgctgggt gcgcttttgc tggttaccag tgccgcctgg gccgcaccag ccaccgcggg
     7261 ttcgaccaat acctcgggaa tttctaagta tgagttaagc agtttcattg ctgactttaa
     7321 gcatttcaaa ccaggcgata ccgtcccaga gatgtaccgc accgatgagt acaacattaa
     7381 gcagtggcag ttacgtaacc tgcccgcgcc tgatgccggg acgcactgga cctatatggg
     7441 tggcgcgtac gtgttgatca gcgacaccga cggtaaaatc attaaagcct acgacggtga
     7501 gattttttat catcgctaaa aaaagccccc tcatcatgag ggggaaatgc agacaccttg
     7561 ttatttttta ttattagcca cttgctcgtc ttgcttgtta ttagtcgtat ttcacgttga
     7621 ttaatgcggt tgcctccagt gcgccagatt taattttatt tgtatcgtag acgtagtaac
     7681 tggcagttat cggaattgaa accgttgtat ctgtaagttt tgacttcgcc gcaacttcct
     7741 ttaaatttgt atatccttta ctgctgttag tgatatttaa aagtgtgact ggcgaaccat
     7801 tactttcaag aataaatcct acgccattat cttccgcacc tttcagtatt gtgttgctgt
     7861 tggttaacaa gtcagaactt gataaataca cctgcatttt ccgtgatgtt tgattttgtt
     7921 gacctaataa atttttacaa cgcagaataa tatcctcttt tttactattt attgttgctt
     7981 tgttacctgt ggctggtaat tgtgaaacgg gaatactgtc taaagtaatt aataaatcat
     8041 ccggtttgca ggttgtggtt atcgggttgt atgacaatgt cagattaaag gaatagacac
     8101 ctgcattttg tgccaatgca tttctgtagc tatttacaca tgcatcccat gacttgccac
     8161 ctaataaaca cagtagaaat tgtactgcgg cactcccgcg tgtcgatgat gaagcactgg
     8221 ataatgtcgc aacaccatta atcatcactg aaccgccgcc ccccgcagaa acattcactt
     8281 ttgcattact ggccgcaggt gtcaccgtaa ctgaataacc actggtgaaa ctggtctgat
     8341 tatttgacaa cgtgatgttg tttttattgg tccattcaaa atgtaaattt aattttatgg
     8401 tatcgttata aagtccaaca ataatatccg acgggctcat aggggtgata gtatttgtac
     8461 ttacgcatga cgaatcatta aaattatgct catatgtcgc catttgtgtt tgagtaagtg
     8521 caaaatcccc ctgaacaacc aagttgtgct gaacagataa ctcactataa catgccgcat
     8581 agctcacagg cataaccatc atcattaata taaatagcat tatgcgaatt tccatagaac
     8641 cacctgacta ttactggcaa atataatttc tactttcatc aatctcttta ccgaaggtga
     8701 ttgtgcatga aagtccttgt tgcttatcaa ttgccacatt aaccgatggc ggtacttcat
     8761 tggtgcgaat aaataactgg cttccctgac cgacaacgcc aatattatga ccatggatat
     8821 cattgacttc ataaccaaac attaatggtt gcccatccgc tcttaacgct tttataaacc
     8881 agggcttgcg ctgatcggta tcaaaattaa ccagtacaac cgcgccgcga taaggtgcgg
     8941 caattttccg gttgccacgt aattctgctt cgctatcgct ttgcgacaca tccagcatca
     9001 ggtgattttc ccgataaggt gtcattccgt cgtataccac cactccatta cggtttgttg
     9061 tgcgatattt ttgcccattg acataagcat ctttaattcc tggcgcattc atcacagcaa
     9121 acgtttcgga aagacggttc gccagattaa cgccacccga ccaggcgaca atgccccctg
     9181 aaacactggc tccagcctgt cgataagtac tcgactgact ataactgcca ttcactgttg
     9241 ccaccggcgc gttccaggtc aaattcgccc cagctgtcgt ttcatttccc tgatgttgat
     9301 gactcaggtt gacaccataa ttgaactgat cccgactccc tactgttcct gataatcccg
     9361 tattatttga ggcaaacccc tgatcatcaa acgtcgttga gttagacata tatatttgcc
     9421 gacgaggcgt cgaaacgtca tcaccccaat caaagggaat cgatataaaa atattaaaac
     9481 gtttctcttc atgatgattc tcgtcataag cctggcttgc cgcgagggta tagcttatcc
     9541 gtcgcaggtt gttggaataa ctcaactgat aatccttact actgccgcta cgcccccagt
     9601 aatctcgcca taacgtactt aatgacacag acccccaacc ttctggcaat gactggctca
     9661 tattggcgga aaagctattt ttgcgaccaa aatcattctg gtaataatcg gcaatgtcat
     9721 agacatcgtt ttcatcacgg cgataattat ctttattgtt tgcccaaacg tgatcgttaa
     9781 atgtccggta atcacgcgac gaataacgcc aggccgccag accaaaacgc gtcgacgttt
     9841 ggctcacaaa tttgttgtag gcaatttgat aactttgccc gtcaaacaca tcgccgttgt
     9901 cttgtttact atgcgacttc gtggcatcga cggaaatggc accaatgcgt gtattccagc
     9961 cagtccccag agtgaacgcg taataattat tcgcaaccat cgagccacca tacaacgtca
    10021 ataagttatt aaaaccatac tgataacccg cctggacaaa atcactatgt ttgctcgccc
    10081 cttcaatatg gctacgaccc gccgcaaaat catatttcga cacgccgggt tgcagcatat
    10141 ttggcaccgc tgcataaggc accagatagg tggttaccga accgtccgcc tctttcacgc
    10201 tgacatcaag atctgctcca ccaccggcca actgcaaatc tgtaatcgca aacgggccag
    10261 gaggaacctc tttctgataa accacaaaac cattctgttc aatagttacc agcgcgttac
    10321 tctgagcaat cccctgcacc cgtggcgtaa aattttgttt cgagttaggc aacatctgca
    10381 tatcacgaaa caaccgcaca cctctgaagc gaacagaatc aaaaatatcg cttgatgtgt
    10441 acatatcacc cacgcgaagc gtgccgagaa gttgggcaaa tccacgttcc agatacaggg
    10501 tattgctttt ccacacccct ggattgttat ttgttttact gaaactggca tcagaatgca
    10561 gttgccaccc cagtaaattt aacccgctgt taaaacgtac atatgtactc ttactattac
    10621 ccgacgcttt atagtcactg taatactgac tcacataata agaggtataa aacgcattaa
    10681 taccccgctc ccagttttcc ggtggaacat agccactttc cagttcttcc acccaggcct
    10741 gcgggacact gaaatcgaga cgaaaaaccc cgatatccca ggtatagctc ccaccctgaa
    10801 caagttgctc aaatgttaaa cattgcttac cgctggcgaa gttatcgcta ttaatgccta
    10861 accgcttgat aacttctctt gataaacatg tttcttgcgg gttgtcttta acaataatct
    10921 catatttccc gcgccattgc ttattgacat aaatatcaat gtcatactgc cccggtaagg
    10981 gttgattatc atcaagacga atatttgcaa cctgctggtc tttcattcca cctatcataa
    11041 aatgggtatc aaaggtttct tcagctgcat aagcttcaat gccgagcaat aacgctacga
    11101 ttgctgatgc aagtggggtc attctcaaca taggcagctc ctgcaattaa attttgtcac
    11161 taatatagtt gccgtggtca tcgataatgg tcagatacca gttatttgca ttattacttt
    11221 tgacattaac actctgactt tctaaggggg caatcattat agtttcataa ttgactttga
    11281 cattattagc tttgacatcc gaaatcgtca cccaattagc tgagtcattt tttatcacca
    11341 atccattgcc actgcgattt accagtaact ttttaaaggt cgctttattt accggagcaa
    11401 tacccgccgg ccggtaaaac aatttgattc tgttttgcat cgcaaacttc agtgcattct
    11461 taccttcttg ttctggacta tttggtggaa tatccagaac attcagataa aaaatgcttt
    11521 ctttattagt gggcagttta ttcggcataa ttttgatttt tacttgctgc ccggaatttg
    11581 cccctatttt tgcaactggt ggcgttaaca tgaaaggaac ctgaattttt tctggtggta
    11641 acgacgtatc gccatcatca atccacgcct gcagcagcga agaacgattt ccctggttca
    11701 tcaactgcac cataacttct ttattttctg ccgggtaaat aatgcgcgtc ccgtagataa
    11761 caattccggc atgtgcagga aggaccatag aaaaaatgag taaagataat aatcctttca
    11821 ttttaatgcc tcttaaatta aaaggccgcc atgggatagc ggccatgttt attacctgga
    11881 gaaacttgca attttattta ttaatcctgc gccacacgca taaccagaga agataacaca
    11941 tcacctgatt gcactgagtt atctgttagt ttcaggtatt ttgctttaaa cggaatagca
    12001 tattgacctt ccgctgtttt ataactctca atattaaaag gtgcagaaac atccaggaca
    12061 tcgctgttat cacctaaata gagatagtaa cccacggtat cgttgctact ggctatcgcc
    12121 ttatcgttgc cggttatttt gtcgttggta atcaacgaga gttttacacc agcaacttct
    12181 ttcgggcagc taatttttaa catgctgctg gttacaccga ctgcatcatc ataggtagaa
    12241 tatttagctt taccttcaaa gacagtggtt ggtacagtcg gcatattaat acttactgaa
    12301 tcaccacccg cttcgaattt acacggagat tctgcaatat ttcctttaat ggttaatgtt
    12361 ccggtatcaa cgtctgcagc aaatactcca gcgctcataa aaaaagaaga aaagacagcg
    12421 gcagcaataa tt
//
LOCUS       DAMZII010000142        12415 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMZII010000142 DAMZII010000000
VERSION     DAMZII010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.142"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            58..1173
                     /locus_tag="POA77_003917"
     CDS             58..1173
                     /locus_tag="POA77_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDI6367280.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1765..2616
                     /gene="hchA"
                     /locus_tag="POA77_003918"
     CDS             1765..2616
                     /gene="hchA"
                     /locus_tag="POA77_003918"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI6367281.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2724..4082)
                     /gene="hprS"
                     /locus_tag="POA77_003919"
     CDS             complement(2724..4082)
                     /gene="hprS"
                     /locus_tag="POA77_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDI6367282.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4082..4753)
                     /gene="hprR"
                     /locus_tag="POA77_003920"
     CDS             complement(4082..4753)
                     /gene="hprR"
                     /locus_tag="POA77_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDI6367283.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKN"
     gene            4886..5299
                     /gene="hiuH"
                     /locus_tag="POA77_003921"
     CDS             4886..5299
                     /gene="hiuH"
                     /locus_tag="POA77_003921"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDI6367284.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYYKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5408..6412
                     /gene="msrP"
                     /locus_tag="POA77_003922"
     CDS             5408..6412
                     /gene="msrP"
                     /locus_tag="POA77_003922"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDI6367285.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6413..7048
                     /gene="msrQ"
                     /locus_tag="POA77_003923"
     CDS             6413..7048
                     /gene="msrQ"
                     /locus_tag="POA77_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDI6367286.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            7305..7955
                     /gene="zinT"
                     /locus_tag="POA77_003924"
     CDS             7305..7955
                     /gene="zinT"
                     /locus_tag="POA77_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDI6367287.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8298..8828
                     /locus_tag="POA77_003925"
     CDS             8298..8828
                     /locus_tag="POA77_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI6367288.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(9397..9486)
                     /locus_tag="POA77_003926"
     tRNA            complement(9397..9486)
                     /locus_tag="POA77_003926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9450..9452),aa:Ser,seq:cga)
     gene            9580..10377
                     /gene="mtfA"
                     /locus_tag="POA77_003927"
     CDS             9580..10377
                     /gene="mtfA"
                     /locus_tag="POA77_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDI6367289.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            10478..10553
                     /locus_tag="POA77_003928"
     tRNA            10478..10553
                     /locus_tag="POA77_003928"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10511..10513,aa:Asn,seq:gtt)
     gene            10733..12007
                     /locus_tag="POA77_003929"
     CDS             10733..12007
                     /locus_tag="POA77_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDI6367290.1"
                     /translation="MSLTDTKVKNARPAEKAVKLTDGFGLYLLVHPNGSKYWQLGYRF
                     DGKQKVFSIGVYPAVSLADARQRRDEAKKLLAQGIDPNAKKQADEKALQEKRDRTRSF
                     RVVARSWFATKTKWSEDYADTVWKRLETYVFPDIGDSNVSDLDTGDLLVPVKKAETLG
                     YLEIAMRIKQYITAILRHAVQQKLMRHNPAYDMEGAVQKPETEHRPALELEEIPLLLE
                     RIDAYKGRGLTTLAIKLNLLIFIRSSELRYARWSEIDFNSKLWVIPEKREAIERVKYS
                     TRGAKMKRQHFVPLCRQALKILKEIRQLTYEEGNEAGLIFTGCYDSFKPMSENTINKA
                     LRKMGYDTTQDICGHGFRTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKH
                     LEQRRLMLQWWADFLDANRDGMVRPFEFAQKQ"
     gene            complement(12074..12325)
                     /locus_tag="POA77_003930"
     CDS             complement(12074..12325)
                     /locus_tag="POA77_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367291.1"
                     /translation="MSKNVKQTSENVASTAAKTLTNPNASAIQKSLAGSALSQRGTSN
                     QTSGQMEHKASSALDNPRSSKLTKQLAASVLAQSNKGRK"
BASE COUNT         3490 a         2831 c         2673 g         3421 t
ORIGIN      
        1 tggataacgg cacttattga tatattcatg aagattataa tcacaaggga atacataatg
       61 aaaagaaaag ttctggcaat gctggtcccg gcgttattag ttgctggcgc agcaaatgcg
      121 gctgaaattt ataataaaga tggcaataaa gttgatttct acggcaagat ggttggcgaa
      181 cgcatctggt caaatactga tgataataac agcgaaaacg aagatacctc ctatgctcgt
      241 ttcggtgtta aaggtgaaac ccaaatcacc agcgaactga ctggttttgg tcagtttgaa
      301 tacaacctcg acgccagcaa gcccgaaggt gaaaaccagg aaaaaacccg tttaaccttc
      361 gccggtttga aatataacga gttaggatca tttgactatg gccgtaacta cggtgttgct
      421 tatgatgccg cagcttatac cgatatgtta gttgaatggg gtggcgattc ctgggcttcc
      481 gctgacaact tcatgaatgg tcgtaccaac ggtgttgcaa cctaccgtaa ctatgatttc
      541 tttggcctgg ttgatggtct tgattttgcg attcagtacc aaggcaaaaa cagcaaccgt
      601 agcactaaga aacaaaatgg tgacggctat gcgttgtctg tagactataa catcaatggt
      661 tttggtatcg ttggtgcata tagcaaatct gatcgcacca acgatcaagt tgccgatgga
      721 aacggttcaa atgctgaact gtggtcgttg gctgcaaagt atgacgcgaa caatgtctac
      781 gcagctgtaa tgtacggcga aactcgtaat atgactccgg gttctattga tacaggtgtt
      841 gccgatcgag aaggcaatac aataatgcgt gatcaactta tcaatgaaac ccagaacttt
      901 gaagcggttg ttcagtatca attcgacttt ggtctgcgtc cgtccttagg ttatgtgtac
      961 tctaaaggta aagatatcaa aggggtacct ggacatagat atgttgacgc tgaccgtgtg
     1021 aactacattg aagttggtac ctggtactac tttaataaga acatgaacgt ttatactgca
     1081 tacaaattca acatgctgga taaagatgac gccgccatta ctggcgcagc agctgatgac
     1141 cagtttgcag ttggtatcgt ctaccagttc taatcagccg acgcgccgcg gcgtgttact
     1201 gtaaaactta acactagcct gagttcgtcc ctcatcgaac tcaggctttt ttattggcat
     1261 ttcatctcca tctgcggcac acgcaattgc caaagcatat cgatacagcc ccttaccctg
     1321 cccctcctgg cacaatatct tctaccagct aatcttcaag ccaacaccca aatgcatttc
     1381 tttatgtatt aatccgtaac aaacacgctt cccatataga aatatttgca aaaatagtgc
     1441 atgaaatatg ctttttctct accaatccgg gatataaata ttcatcagca ccacaatatt
     1501 ccacatcact aaatctctcc ccgccaccct gtacctctga taatggtcta aaatcattga
     1561 ggccacttgc gacgacgttg ctcaataagg aaaatttagc gtgaataata ttcaacacca
     1621 gtgcagcatc atcattacat acttacaccc gccgactacc gctttcgata tattgactca
     1681 attttccagc ctggttaaaa atagcgacca gctcagtcgc aaatatagtg actaccctaa
     1741 ctaagcaaca ataaggaata cactatgact gttcaaacaa gtaaaaatcc gcaggtcgat
     1801 attgctgaag ataatgcatt cttcccttca gaatattcgc ttagccaata taccagtccc
     1861 gtctctgatc ttgatggcgt ggactatcca aaaccgtatc gcggtaaaca taaaattctg
     1921 gtgatcgccg cggacgaacg ttatttgccg accgataacg gaaagctgtt ctcgaccggt
     1981 aaccatccga ttgaaacgtt gctgccgttg tatcatctcc atgctgcagg tttcgaattc
     2041 gaagtggcga ccatttccgg tctgatgacc aagtttgaat actgggctat gccgcacaaa
     2101 gatgaaaaag taatgccatt ctttgagcag cataaatcgt tgttccgcaa tccgaagaaa
     2161 ctcgcggatg ttgtcgccag cctcaacgct gatagcgaat atgcagcaat ctttgttcct
     2221 ggtggtcatg gcgcacttat tggtttacca gaaagccagg acgtagctgc tgctttgcag
     2281 tgggcaatca aaaatgaccg ttttgttatc tccctttgcc acggcccggc ggcttttctg
     2341 gcgcttcgcc acggcgataa cccactgaat ggttattcca tttgcgcatt cccagacgcc
     2401 gcagacaaac aaacgccaga gataggctat atgccgggtc atctcacctg gtacttcggc
     2461 gaagagctga agaaaatggg catgaaaatc attaatgacg acatcaccgg gcgagtacat
     2521 aaggaccgta aagttctcac cggcgacagt cctttcgcag cgaatgcgtt gggtaaactg
     2581 gcggcgcagg aaatgctggc agcttatgcg ggttaatccc tgttacgtat gaatgtaagc
     2641 gcataatcaa tcgcagccac gttaatactc acgacgtggc tgcgtttttt catggttgat
     2701 ttatggcgta ctgaagccct atgttaattt ctttgcggta acgtaatccg gaacacatta
     2761 tgcttattga gatagtgata cgtagcactt cccccatgta attcggcaat cgctttgact
     2821 aaagaaagac caagtccctg acctacggaa tgacgcgaat tatctccccg ccaaaatcta
     2881 cggaagagtt tttcaggctc atgaattttc gttccaggac tggcgacatc aatattaaga
     2941 tagccgttgg tatcaagaaa actggttata tgaatacgcg atttttctgg tgaatatcta
     3001 atggcattaa caataagatt cgataacatt cgttgcagta aaattttatc cgcaaagatt
     3061 tgctgattgc actcgacctt aaagcaaatc tctttctcgt ctgaaaggta ttcaagataa
     3121 tccaacaaat tttcgacttc cttattgaga gaaagcgagt ccagtttcac caaaacattg
     3181 tttttatctg cccgggcaag aaacagtatg ttctctgtta accgcgaaat attttccagc
     3241 tcttcaatgt taccggcaat tgttttttga tattcagcga tacttctggt ttgactgagc
     3301 gtaacctgat tctgacccag taatgcatta atgggcgttc taagttcatg agcgagatcg
     3361 tcagcaaatt gacttaggcg ttcaaaatct ttgactaagg cttgatgcat tttattcaac
     3421 gcctgcccaa gaggttttag ttctctcggt aatgcgctaa cctcaacagg ctcccggcta
     3481 tcgttataat tcagcgcttc cgttacacca ctcaactttt tgatctctcg taatcccgtt
     3541 ctgattaaca gcggacttaa tactgagcaa agtacaatgg cgacaatgca aattataatg
     3601 ctattaattt tatactgttc aagcatgtta tgtctggctg aagccaattt agccacagta
     3661 accgttaatg atggcgaaac ttcatcaata ttaattcgta aagcatcaat ctcagtatca
     3721 ttaataatgc ttctgtaaat gccagctgcg ctgattgtct cactagcagg tatgttattt
     3781 aacatgtcat cactgacatt tgtccggtta acaattttat tgatgctatc accatgaatg
     3841 atcaagatat cctgactaac atccatcatc cggttaaagt atacaggtaa cgtatctgga
     3901 tttaccccat caattaacaa ctgcttgatc tgtgctgtcc ggttaatgag tgttgtatca
     3961 tcgcgccatt tcaactcact tgccaggcca ttatagagag tccagacaat tccagcgcca
     4021 gcaacagaca gcagcaatat aaaaagcaag gttaaacgga cggttataga taatcttttc
     4081 attaattttt taccgctacg aatgaatagc ccatcccccg gattgtggcg attagctttt
     4141 caggaaaagg atcatcaact tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg
     4201 tatcactatc aaagttgatt ccccaaattt cactcgcaat aaccgttctg ggtataattt
     4261 cgccagctct ggaggccagt agccaaagta actgaaactc cttgcgcgtc agtgtaatac
     4321 tgatattgtc cctgctcaca ctttgactta cagagtccat tcttaagcca ctgatttcta
     4381 atgttgaatt caaagcgtga tgttgcctta attgtgcccg aacccttgcc agcaactcag
     4441 aaaatgaaaa aggttttacc agataatcat ttgctccact gtccagccct ctgactctgt
     4501 catcgacaga atcccttgca gtaaggcaaa taacaggggt ttgctttgct gttcttaacg
     4561 tttgtaagat ctgccagcca tccatacccg gaagcataat atccagaatg atcaatgcat
     4621 aatcatcctt cagcgcaaga taaagcccat ctctgccatc agaaacggca tcaatgacat
     4681 aacccgcttc ggaaagcccc tgcgttaccc actcctgggt cctttgatta tcttcaataa
     4741 gtaaaatctt catgaaaata atatgccata aatcatatgt tatagaaaca gcctggttca
     4801 ttacaaaatt gtaatgctgc tgtaaggtta ccctggccgc tttttcgcta tcctcaaaac
     4861 tcattcacat gacaaggata taaacatgtt aaagcgttat ttagtactct cggtagcaac
     4921 ggcagcattt tcattacctt ctttggttta tgccgcacaa caaaacattc ttagcgtgca
     4981 cattttgaac cagcaaaccg gaaaacccgc tgctgacgtg acagtcactc ttgaaaagaa
     5041 agcggataac ggctggttac aacttaatac cgccaaaaca gataaggatg gacgaattaa
     5101 ggcactgtgg cccgagcaaa ctgcgactac tggcgattac cgtgtcgtat ttaaaaccgg
     5161 ggactattac aagaaacaaa atcttgaaag tttcttccct gagatccccg ttgaatttca
     5221 tattaataaa gtgaacgagc attatcatgt acctttatta cttagccaat atgggtattc
     5281 aacctatcgt ggcagttaaa gcataaatat gtaaaagcag atctctgcta ctgttaggga
     5341 ggacactccc tgacagatta acagtaaacg gctcttgctg gctaacgaca aaaaagtgtg
     5401 atggcttatg aaaaagaatc aatttttaaa agaatcagat gttacggccg agtcggtatt
     5461 ctttatgaag cgtcggcagg tgttaaaagc actgggcatc agcgcagctg cactttcttt
     5521 gcctcacgct gcgcatgccg atctgcttag ctggtttaaa gggaacgatc gcccgcccgc
     5581 ccccgccgga aaaccgctgg agttcagcaa gcctgccgcc tggcaaaata acctgccact
     5641 gacgccagta gataaagtct ccggttataa caacttctat gaattcgggc tggataaagc
     5701 cgatcccgcc gctaatgctg gtagcctgaa aaccgatcca tggacactga aaatcagcgg
     5761 cgaagtggca aaaccattga ccctcgatca cgatgattta acccgtcgct tcccgctgga
     5821 agagcgtatt tatcgtatgc gctgcgtgga ggcatggtcg atggtggtgc cgtggattgg
     5881 ttttccgctg cacaaattgc tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc
     5941 tttcgaaaca atttatgcac cggaacaaat gccaggccag caggaccgct ttatcggcgg
     6001 cgggctgaaa tatccttatg tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac
     6061 actgatgacc gtgggtgttt atggcaaggc gttaccgcca caaaatggcg cgccggtacg
     6121 actgattgtg ccgtggaaat atggctttaa agggattaaa tcgatagtca gtattaagct
     6181 gacccgcgag cgtccgccaa ccacctggaa tctggcagcg cctgacgaat acggttttta
     6241 cgccaacgtt aatccgcatg ttgatcaccc gcgctggtca caggctaccg aacgatttat
     6301 tggttcaggc ggcatcctcg atgttcagcg ccagccaacg ctactgttta atggttacgc
     6361 cgaccaggtg gcatcgctgt atcgtggcct ggatttgcgg gagaatttct gaatgcgtct
     6421 gacagcaaaa caggtgacat ggctgaaagt tagcctgcat cttgccggat tgttgccgtt
     6481 tctctggctg gtctgggcga tcaatcacgg tggactgggt gccgatccgg tgaaagatat
     6541 tcagcatttt accggtcgca ctgcactgaa atttttgctg gcgaccttgt taatcacacc
     6601 tctggcacgc tacgcaaaac agccgttatt gatacgcact cgccgcctgt taggcttatg
     6661 gtgctttgcc tgggcgacac tgcatttaac cagttacgca ttgctggagc tgggcgtgaa
     6721 taatctggcg ttattaggca aggagttaat tacccggcct tatttaacgt taggaattat
     6781 cagctgggtt attttgcttg ctttagcgtt tacttcaacc caggcgatgc agcgaaaact
     6841 cggcaagcac tggcaacagt tgcataattt cgtctatctt gtcgcgatcc tggccccaat
     6901 acattatctg tggtcagtga agattatttc accgcagccg ctcatctacg ctgggctggc
     6961 tgtactgctt ttagccttac ggtataagaa gttgcgttcc ctttttaacc ggttacgcaa
     7021 gcaggttcac aacaaattat ctgtataact tcatattacc gacaaagtac gcgttatccc
     7081 tgccggatgc ggcgcgagcg ccctatccgg cctacgcaga tcacatcagg ttatgtttcg
     7141 tttgtcatca acctcaaatt atcaacataa ttcattttat ttcaataaga tagataagta
     7201 tacctgagaa aactatgctc tcatctccta agagttattg cattttgtca tatgttacaa
     7261 tataacatta cacatcatat acaatgactc tggaggaaac tgttttggcg attcgtcttc
     7321 acaaactggc tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc
     7381 atcactcaca cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg
     7441 atgatgccaa tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt
     7501 atcctttact gcaaagtggc aaacttgacc ccgtctttca gaagaaagcg gatgcagata
     7561 aaactaaaac atttgctgaa attaaagatt attatcacaa aggttatgca acagatatcg
     7621 agatgattgg cattgaggac ggcattgttg aattccatag aaataatgaa acaacatcct
     7681 gtaaatatga ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc
     7741 gttacttatt cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg
     7801 atcatataat tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattcac
     7861 agcaatcttt attaaatgaa atggaaaact ggccaacgta ttatccatat cagttgagta
     7921 gcgaagaagt ggtcgaggaa atgatgtctc attgagtatt ctcatgataa cgcctcgatg
     7981 ccgctttagt aagtcatcat aactgccact ggtccatcca caaacgccac tgaacgcaag
     8041 ctagctacag acacgctcat cactatgacg tgtctgtata ttaataagct aacccgcatt
     8101 gagttaacca ataacggatt ccatacacaa tacggccaac agagaaagat acctgtgctc
     8161 acgccattgc ttatattggc tggttacaat gtgcactatc aattttttaa ataacaaatt
     8221 cttaaaacca catctgtata aggatttttt aaggttcgtg ggatagcttg actgtaaaaa
     8281 ttacaggagc tacaaaaatg aaccgattct caaaaactca aatttattta cattggataa
     8341 cgctgctttt cgttgcaata acctatgccg cgatggaact ccgtggctgg tttcctaaag
     8401 gtagtagtac ttatctgctg atgcgagaaa cacattacaa tgcgggtata ttcgtttggg
     8461 tgttaatgtt ttcacgcctg attataaaac accgttatag tgatccttct attgtaccac
     8521 cgccacctgc ctggcaaatg aaagcggctt cgctaatgca catcatgctt tatataacct
     8581 tccttgcact tcctctgctg gggattgctt taatggctta cagtggaaaa tcgtggagtt
     8641 tccttggttt caatgtgtct ccctttgtta ccccaaacag cgaaattaaa gcactgataa
     8701 aaaatattca cgaaacctgg gcaaatatag gttacttttt aatcgcagct cacgctggcg
     8761 cagcactctt tcatcactac attcagaaag ataatactct gttacgaatg atgcctcgcc
     8821 gcaaataagt cgatcccctg acaaggaaat gtgaaccata gacatcaacg cgtcataaat
     8881 gtttacgcaa gtgcacaatg caccctttcc actatcaaca aggaggagca gaaactgtgt
     8941 aaatcgtaca atggtaagac ttatcaaaaa tacaggttaa ttttggtatt taaacctttt
     9001 ttacttgtta gtatgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga
     9061 aattatggac tttgtcccgc ggctttaatc tgacggccgc gttccttttt tgactcaatt
     9121 attgacccct tctctacgca acttcagttc ccaccaccaa ctttgcggca gtttcatagg
     9181 atgaatgtct aaaagaataa tggtgagcga taagaaaacg actgaataac tgcacatttt
     9241 cgctcgaaag cttcctgtca gatccatagg aatcaagtgc tgaatgtcac agtatcgaac
     9301 agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt ttaggaaaag
     9361 caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac
     9421 ccccggtaga gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat
     9481 ctctccgttc agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgtcc
     9541 gtgcaatttt gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa
     9601 gtacaagaat cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt
     9661 ttaacgtgtc tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta
     9721 cagcaaaagc ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg
     9781 atagcacttc tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt
     9841 catgaagtct taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc
     9901 ggtctggtgc ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc
     9961 gttttgaact ggttggatat acaagattct tttgatgctt ctggttttaa cctgattatt
    10021 catgaagtcg ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt
    10081 attccgttgc gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt
    10141 caggaagaaa tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgctgccagt
    10201 gatcctgctg aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt
    10261 gctcctcgtt tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg
    10321 cagagactgc atcgcactaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta
    10381 acaactttgc agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca
    10441 aggtaccgct aagtaatatt cgccccgttc acacgattcc tctgtagttc agtcggtaga
    10501 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaatttaag
    10561 ggaagcagac gttcactgac gtctgctttc tgcatttata tcaactggtt attcccttct
    10621 ttaggttcac cctcgttcac taaaaaccac tcgaagccat ccccttttgc tggtaaaaat
    10681 gctggtaaag ctggttcgat ttgcgtttta ccagcacgcg gagggaaccg tcatgtcact
    10741 gactgatact aaagtaaaaa atgccagacc agcggaaaag gccgtcaagc tcactgacgg
    10801 gtttggcctc tacctgctgg tgcatcccaa cggttcgaaa tactggcagt taggctatcg
    10861 cttcgatggc aaacagaagg tgttttccat tggggtttac cctgcggttt cacttgctga
    10921 tgccagacaa cgacgtgatg aagcaaaaaa gctgcttgct cagggaatcg atcctaacgc
    10981 taaaaaacag gctgatgaaa aagctctgca ggaaaagcgg gatagaaccc gttcgttccg
    11041 tgtcgttgcc agaagctggt ttgccaccaa aacaaaatgg tctgaagatt acgccgatac
    11101 ggtatggaag cgccttgaga cttatgtatt cccggacatt ggcgacagca acgtttcaga
    11161 tctggacacg ggtgatctgc ttgttccggt taaaaaagcg gaaacgctcg gctatcttga
    11221 aattgccatg cggatcaagc aatacatcac tgccattctg cgtcatgccg tccagcaaaa
    11281 gcttatgcgt cataatcctg cttacgatat ggaaggtgcg gtgcagaaac cagagactga
    11341 gcaccgtcct gcactggagc tggaagagat ccccctgcta cttgaacgta ttgatgccta
    11401 caaaggccgt ggacttacca cactggcgat taaactcaat ctgttgattt tcattcgttc
    11461 cagtgaactc cgctatgcac gatggtcaga aatcgacttc aacagtaagt tatgggtgat
    11521 accagaaaag cgagaagcga ttgagcgcgt caaatattcc acgcgtggcg caaaaatgaa
    11581 acgccagcac ttcgttcccc tctgcaggca ggctcttaag atactgaaag agatccgtca
    11641 gcttacctat gaagaaggta acgaagccgg attaattttc actggctgtt atgactcgtt
    11701 caaacccatg agtgaaaaca ccatcaataa agcactacgt aagatgggct atgacaccac
    11761 gcaggatatt tgcggtcatg gtttccgcac actggcatgt agtgctctga ttgaatcagg
    11821 gctatggtct gaagatgctg tagagcttca gatgagccat aaggaaagca acagtgtccg
    11881 tgctgcctat acccataagg ccaaacatct tgagcagcga aggttgatgc tacagtggtg
    11941 ggcagatttt cttgatgcta accgagatgg gatggtcagg ccgtttgagt ttgctcagaa
    12001 acaataaccg tcatccagca aacaatcgca gtaaataaaa aacgcaggga ggttttactc
    12061 tccctgtaat ttgtcactta cgccctttgt ttgattgtgc caaaacagag gctgcaagtt
    12121 gcttcgttaa cttgctggaa cgtggattat caagagctga tgatgcctta tgctccattt
    12181 gcccacttgt ctggtttgat gtcccacgtt gcgaaagcgc agaaccagcg aggcttttct
    12241 ggatggcaga ggcattagga ttagtcagcg tttttgcagc agtagaagcc acattttcag
    12301 aagtctgttt tacgttttta ctcatgaaac gaatccccaa gaagtcatga acatcactta
    12361 tgtagtcttt tcatcaaaaa aaaatcacta catgcagtga tagccatgtt cttta
//
LOCUS       DAMZII010000143        12235 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZII010000143 DAMZII010000000
VERSION     DAMZII010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.143"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="POA77_003931"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="POA77_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDI6367292.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="POA77_003932"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="POA77_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDI6367293.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="POA77_003933"
     CDS             complement(2659..5772)
                     /locus_tag="POA77_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI6367294.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="POA77_003934"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="POA77_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDI6367295.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="POA77_003935"
     CDS             7014..7292
                     /locus_tag="POA77_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367296.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="POA77_003936"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="POA77_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDI6367297.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="POA77_003937"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="POA77_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDI6367298.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9447..9779
                     /gene="hdeA"
                     /locus_tag="POA77_003938"
     CDS             9447..9779
                     /gene="hdeA"
                     /locus_tag="POA77_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDI6367299.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9895..10221
                     /gene="hdeB"
                     /locus_tag="POA77_003939"
     CDS             9895..10221
                     /gene="hdeB"
                     /locus_tag="POA77_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDI6367300.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10285..10932
                     /locus_tag="POA77_003940"
     CDS             10285..10932
                     /locus_tag="POA77_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDI6367301.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLLNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10974..11504)
                     /locus_tag="POA77_003941"
     CDS             complement(10974..11504)
                     /locus_tag="POA77_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6367302.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11660..12226)
                     /gene="slp"
                     /locus_tag="POA77_003942"
     CDS             complement(11660..12226)
                     /gene="slp"
                     /locus_tag="POA77_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDI6367303.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3376 a         2727 c         2784 g         3348 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc tcgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggtatagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagttt gtaaacagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaatgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac tgcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacgctgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca acagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aaatatctga ttttgatatt ttctatcaac
     9361 atgacatata cagaaaacca ggttataacc tcagtgtcga aattgattcg tgacggctct
     9421 ttcactttat agttgaggat attacgatga aaaaagtatt aggcgttatt cttggtggtc
     9481 tgcttcttct gccagttgta agcaatgcag cggatgcgca aaaagcagct gataacaaaa
     9541 aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa
     9601 ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa accagaagat gcggttttag
     9661 atgttcaggg tattgcaacc gtaaccccag ctatcgttca ggcttgtact caggataaac
     9721 aagccaactt taaagataaa gttaaaggcg aatgggacaa aattaagaaa gatatgtaat
     9781 tccgggaatg cgttacatcg tacttccttg catattgaac aggccggaat atcttcttta
     9841 aaagcagcta ttcctcctgt tcatatataa tctctatatt gaatgggtta caaaatgaat
     9901 atttcatctc tccgtaaagc gtttattttt atgggcgctg tagcggcttt gtcactggtg
     9961 aacgcacaat ctgcgctggc agccaatgaa tccgctaaag atatgacctg ccaggaattt
    10021 attgatctga atccaaaagc aatgaccccg gttgcatggt ggatgctgca tgaagaaaca
    10081 gtatataaag gtggcgatac cgttacttta aatgaaaccg atctcactca aattcctaaa
    10141 gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt ataccttcaa aaatcaagca
    10201 tctaatgact tgccgaatta atgaggtaca agtaaaaagg agtagcaagt tgagccatct
    10261 tgctgctcct ttttgcattt ttatatgaca gcagaattta ttatacgtct tatactcgcg
    10321 gcaattgcct gtggcgctat tggcatggaa aggcaaatgc gcggcaaagg agcagggtta
    10381 cgcacacatg tattaattgg catgggaagc gccctgttta tgattgtttc gaaatatggt
    10441 tttgctgacg tgctgtcttt agatcacgtc ggactcgacc ccagccgtat tgctgctcag
    10501 gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt
    10561 gtaggtctga cgacggcagc ggatatctgg gtgaccgccg ccataggtat ggttattggc
    10621 agcggtatgt acgaactggg tatttatggt tcagtgatga ccttgttggt gctggaagtc
    10681 ttccatcaat taaccttccg cctgatgaac aaaaattacc atttacagct gacattactg
    10741 aacggtaata cggtatcaat gctcgactgg tttaagcagc aaaaaataaa aacggattta
    10801 gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca ttgatatcca gcttcatgcc
    10861 acaacgtcga tagaagacct gttgcgatta ttaaaaggta tggcaggggt gaaaggcgtt
    10921 tcaattagct aatctgtgtg gcagcgtagc cagactcacc gtaagcctga aattcacacc
    10981 agataatcaa tatgctgatg gcgtaccagc tcattgatcc gcttaagatt gaggatcatc
    11041 atgatattgt gtttatggca gtagaacgtt tttagcgata ttttcagttt ctccagaata
    11101 tcttccgttg tcatttgatt cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt
    11161 gacacagccg cttcaggcac agagcagtaa ctgccattga tgagtgcgac aaaatcaatg
    11221 atagtgatag tccgcggaac aaaggtcacc ggcactttcc ccaggcagcg tttaatatta
    11281 aagggggaga aaataatcac atggacaggt tttaatcgtt caatttgagt gagaaaatta
    11341 gaatgaaata cattattcat taatgtatca ataatgacga aggcattctg atgcaacatt
    11401 acgtcgatct cttcttcgtt ctgtatatga acgacattac ctttactcag aatgtttttc
    11461 atcgcggtga agaacatcgt atccctggta attataagaa acataaccga ctcctggtgc
    11521 ggacattaac tttataatca gtttgccaaa ggtaattaac aggacagggc ttataataat
    11581 aatagttagc ttactgtata ttaattttat gtaatacatg atgcagatgc tgagaaagca
    11641 tctgcatctt tcggtggtgt tatttgacca gctcaggtgt tacctgactc accgcattgg
    11701 tgtagtaagg cgcaccccag cccggttccg gccagaatgc accatagccg taatcccaca
    11761 ggttataggt ggtattaacc acttctctca aatgccacac ctggatgccc tgcatattca
    11821 cttccaggaa gttatacggg actttattga taaagcctgg ttgttcaccc tgaatggtac
    11881 cgaggatggt aacaaagtga ttacgatagt tcactggatc aaggaagccg ctttgtctgg
    11941 cgagcagtcg gccctgatag ttggcttcaa tatcaggctt cgcatagcta tccaacggta
    12001 atacagcgat ttctaacaac gtatccgttt tgccattgat aacgttgata accttcccac
    12061 caaagcgcgc ttgttgacca acatataacc ccggctggtt atgaacagca acaaaacttt
    12121 tttgaatatc aggttggtta ttgcctttga tattttgcgg aattgaacta catgcggcaa
    12181 gcaaaaatga aaggctgagg atgagtgcac cttttgtcat gttcatgtta ctatc
//
LOCUS       DAMZII010000144        12114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZII010000144 DAMZII010000000
VERSION     DAMZII010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.144"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            163..597
                     /gene="slyA"
                     /locus_tag="POA77_003943"
     CDS             163..597
                     /gene="slyA"
                     /locus_tag="POA77_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDI6367304.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(644..1111)
                     /gene="slyB"
                     /locus_tag="POA77_003944"
     CDS             complement(644..1111)
                     /gene="slyB"
                     /locus_tag="POA77_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDI6367305.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1385..2506
                     /gene="anmK"
                     /locus_tag="POA77_003945"
     CDS             1385..2506
                     /gene="anmK"
                     /locus_tag="POA77_003945"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDI6367306.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVITTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANPRYQS"
     gene            2592..2921
                     /gene="mliC"
                     /locus_tag="POA77_003946"
     CDS             2592..2921
                     /gene="mliC"
                     /locus_tag="POA77_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDI6367307.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2980..3636
                     /gene="pdxH"
                     /locus_tag="POA77_003947"
     CDS             2980..3636
                     /gene="pdxH"
                     /locus_tag="POA77_003947"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDI6367308.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3765..5039
                     /gene="tyrS"
                     /locus_tag="POA77_003948"
     CDS             3765..5039
                     /gene="tyrS"
                     /locus_tag="POA77_003948"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI6367309.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5098..5961
                     /gene="pdxY"
                     /locus_tag="POA77_003949"
     CDS             5098..5961
                     /gene="pdxY"
                     /locus_tag="POA77_003949"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDI6367310.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(6005..6610)
                     /gene="gstA"
                     /locus_tag="POA77_003950"
     CDS             complement(6005..6610)
                     /gene="gstA"
                     /locus_tag="POA77_003950"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDI6367311.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6716..8218)
                     /gene="dtpA"
                     /locus_tag="POA77_003951"
     CDS             complement(6716..8218)
                     /gene="dtpA"
                     /locus_tag="POA77_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDI6367312.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8829..9464)
                     /gene="nth"
                     /locus_tag="POA77_003952"
     CDS             complement(8829..9464)
                     /gene="nth"
                     /locus_tag="POA77_003952"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI6367313.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9464..10159)
                     /gene="rsxE"
                     /locus_tag="POA77_003953"
     CDS             complement(9464..10159)
                     /gene="rsxE"
                     /locus_tag="POA77_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDI6367314.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(10163..10783)
                     /gene="rsxG"
                     /locus_tag="POA77_003954"
     CDS             complement(10163..10783)
                     /gene="rsxG"
                     /locus_tag="POA77_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDI6367315.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(10787..11845)
                     /gene="rsxD"
                     /locus_tag="POA77_003955"
     CDS             complement(10787..11845)
                     /gene="rsxD"
                     /locus_tag="POA77_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDI6367316.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11846..>12114)
                     /locus_tag="POA77_003956"
     CDS             complement(11846..>12114)
                     /locus_tag="POA77_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367317.1"
                     /translation="AAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKA
                     RKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         3114 a         3024 c         3062 g         2914 t
ORIGIN      
        1 ttaataaata ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac
       61 ggtaaccaaa tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa
      121 agccgcataa tatcttagca agctaattat aaggagatga aattggaatc gccactaggt
      181 tctgatctgg cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg
      241 ctggagttaa cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac
      301 cagtcgcaaa ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtact
      361 ctggaccaac tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg
      421 gctaaacgta ttaaactgac ggaaaaggca gagccactga tcagcgaaat ggaagctgtt
      481 attaacaaaa cccgcgcgga aatattacat ggcatctccg cagaggaact ggagcaactg
      541 attacgctca tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat
      601 gaagggggcg tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggagaaacgg
      661 tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa cgagtgttgc
      721 cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc agctcgacac
      781 cctgcgtttt gttcattgca ctctgtacgc cctgaccagc tacgccacct gcaacagcgc
      841 ctgctgcagt agccagagaa cgcccggttc cgccaccaac agtattcccc aggaaaccac
      901 caagaacagc accgccaatt gcaccgataa cgttggaatc atcaccgccc tgaatctgta
      961 ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc gcttcagaag
     1021 cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta
     1081 ccattgaaac aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac catgaaacgc
     1141 cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg ttacatgttc
     1201 taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac aaaacattca
     1261 tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg gtcattccgc
     1321 atggtattca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg
     1381 aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg atggtgttga
     1441 tgttgtgttg gcgacaatag atgaacaccg ggtcgcacag ctggcaagct tgagctggcc
     1501 gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc agcttacact
     1561 ttcgcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg cggtcaatgc
     1621 cttgcttaag gaacaaaacc tgcaggcgag agatatagtt gcgatcggct gtcacggtca
     1681 aaccgtctgg catgaaccga cgggcgtggc accacacact ttacagattg gcgataacaa
     1741 tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc gcgatattgc
     1801 cttgggagga caaggcgcac cgctggtacc tgcgttccat catgccttgc tggctcactc
     1861 aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac tgctcattcc
     1921 tgggcagccg gttggcggct acgataccgg tcctggtaac atgctgatgg atgcctggat
     1981 ctggcgtcag gccggtaaac cttacgataa agatgccgag tgggcacggg cgggtaaagt
     2041 tattctccca ctgctgcaaa atatgctcag cgacccatat ttctcgcaac ctgcaccgaa
     2101 aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttac gccattttcc
     2161 gggtgttgat ccccgtgatg ttcaggccac actggcagaa ctcaccgccg tgaccatttc
     2221 tgaacaagtt ttgttgagcg gtggctgcga acgattgatg gtatgtggtg gaggtagtcg
     2281 taatccgcta ctcatggcgc gtctggcggc attactgcca ggcacagaag tcatcaccac
     2341 cgatgccgtt ggcattagtg gcgatgacat ggaagcattg gctttcgcct ggcttgcctg
     2401 gcggacgctg gcgggattac caggaaatct gccttccgtc actggcgcaa gccaggagac
     2461 ggtactgggg gctattttcc ctgctaaccc gagatatcag agttaactga atttttctta
     2521 ccgcgtctgc cgttacactt ggaacgttag ggagggcgta ttgccctcca gaccaggaaa
     2581 gtcttcggga aatgtctatg aaaaaactgt tactgatctg tttgccggtg cttctcaccg
     2641 gctgtagcgc gttcaatcaa ctggttgagc acatgcaaac cgctacgctg gaataccagt
     2701 gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc agttttgttt
     2761 acgataatca actactgcat ctcaaacagg gcatttcagc gtctggtgca cgttacactg
     2821 acggaatcta tgttttctgg tcgaaaggcg atgaagcgac tgtctataaa cgcgaccgca
     2881 tcgtcttgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc aggggcggcg
     2941 cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa cgacgaattg
     3001 cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg tcgcgatctt
     3061 cccgccgatc cattaaccct ttttgaacgt tggctctctc aggcgtgtga agccaaactg
     3121 gcagacccta ccgcgatggt ggtcgctacc gtggatgaac atgctcagcc ttatcaacgc
     3181 atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa cctcggtagc
     3241 cgtaaagcac atcaaattga aaataatccg cgcgttagcc tgctgttccc gtggcatacc
     3301 cttgaacgcc aggtgatggt gatcggtaaa gcagaacgac tttcgactct cgaagtgatg
     3361 aaatattttc atagccgccc gcgtgatagc cagattggtg catgggtttc gaagcagtcc
     3421 agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa gcagaagttt
     3481 caacagggcg aagtgccatt gccgagcttt tggggcggtt ttcgcgtcag ccttgaacag
     3541 attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta ccagcgagaa
     3601 aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa
     3661 tcgctggtac tcctgattct ggcactttat tctatgtctc tttcgcatct ggcgaaaagt
     3721 cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca agcagtaact
     3781 tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag gaagcgttag
     3841 cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct accgctgaca
     3901 gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag caggcgggcc
     3961 acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg agcttcaaag
     4021 ctgccgagcg taagctgaac accgaagaaa ctgttcagga gtgggtggac aaaatccgta
     4081 agcaggttgc cccgttcctc gatttcgact gtggagaaaa ctctgctatc gcggcgaaca
     4141 actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact
     4201 tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac cgtgaagatc
     4261 aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac ttcgcctgtc
     4321 tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc tgaccagtgg ggtaacatca
     4381 cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc ctgaccgttc
     4441 cgctgatcac taaagcagat ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt
     4501 tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac actgcggatg
     4561 ccgacgttta ccgcttcctg aagttcttca cctttatgag cattgaagag atcaacgccc
     4621 tggaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgta ctggcggagc
     4681 aggtgactcg tctggttcac ggtgaagaag gtttacaggc ggcaaaacgt attaccgaat
     4741 gcctgttcag cggttctttg agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg
     4801 acggcgtacc gatggttgag atggaaaagg gcgcagacct gatgcaggcg ctggtcgatt
     4861 ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat gccatcacca
     4921 ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat cgtctgtttg
     4981 gtcgttttac cttactgcgt cgcggcaaaa agaattactg tctgatttgc tggaaataat
     5041 gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac agggaacatg
     5101 atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg taacagtgcg
     5161 gcagagtttc ctatgcgccg cctgggcgcg aacgtctggc cgctgaacac cgttcaattt
     5221 tctaatcaca cccaatacgg caaatggact ggctgcgtga tgccgcccag ccatttaacc
     5281 gaaattgtgc aaggcattgc cgccattgat aaattacaca cctgtgatgc cgtgttaagt
     5341 ggctatctgg gatcggcgga gcagggtgaa catatcctcg gtatcgtccg tcaggtgaaa
     5401 gccgcgaatc cgcaggcgaa atatttttgc gatccggtaa tgggtcatcc ggaaaaaggc
     5461 tgtatcgttg caccgggtgt cgcagagttt catgtgcggc acggtttgcc tgccagcgat
     5521 atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt aaataacgtc
     5581 gaagaagcgg ttctggcagc gcgcgaactc attgcgcaag ggccacaaat tgtgttggtt
     5641 aaacacctgg cgcgagctgg ctacagccgt gaccgttttg aaatgctgct ggtcaccgcc
     5701 gatgaagcct ggcatatcag ccgtccgctg gtggattttg gtatgcgcca gccggtaggt
     5761 gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc aacgctgcag
     5821 gaggcgctgg aacatgtgac cgctgcagtc tacgaaatca tggtgaccac caaagcaatg
     5881 caggaatatg agctgcaagt ggtggctgct caggatcgta ttgccaaacc agaacattac
     5941 ttcagcgcaa caaagctctg aaatatctca tttcggccca caacgacctg tgggccgatg
     6001 gatattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt tcagccatac
     6061 gttgcataaa tgctgcaatg tgctctaacc cttccagatt cagtttcacc gcgtatgccc
     6121 agcgcagaac cgtaaacaga taggcatcag caattgtaaa tctttgcccg cagatccagt
     6181 gctcatcctt cagtgcctcg ttcacatatt gcagcttctt ctccagctgc gcgcgaactg
     6241 tcggtttgta ctcttccggt gtatcagggc gaaacagagg tgtgaaacct ttatgcagct
     6301 cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg tttaccggtg
     6361 ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgct acgccttccg
     6421 tcagcaaagt accgtcatcc agcagcaatg caggcacctg ccccttaggg ttaacggcaa
     6481 agtaatcgtc accgttttcg agacgttttt tcattaaatc cacactgacg agggtaaaat
     6541 cctttccgct ctcacgcagg gtgatatggg aagcgagaga gcaggcaccc ggtttgtaga
     6601 acaatttcat cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc gttgctgaac
     6661 aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa gagtttccct gaattttacg
     6721 ctacagcagc tttcgccgct ttgtctgcag cgtcatcctg cgtcatacgg tgcagtttag
     6781 gcgcggtcag cagcatcagt actgcaataa tggcagtagc gacaccaatc tgcaagaata
     6841 cgcgaccata aacttccagt gacatcagcg ggtcggtaac gttatccggc acagccatca
     6901 tacccgcaac ataaccacca atcaggtttg caccggcagt ggtcaggaac cagctaccca
     6961 taatgaagcc catcagacgc tgcggaacga gttgtgcaac cattgccaga cccagaccag
     7021 agatcatcag ttccccgatg ctctgcaggc catagcttgc gaccagccag cttacagaca
     7081 cgataccagc gtcagacgcg aatttcgcac ccagcggcag aatcaggaac gcaccagaac
     7141 acatcaccat gccgattgca aacttggttg gcatcggcag ggtatcgccc atcttgttat
     7201 agatagcggc cagaatcgga ctaccgatga tgatccagaa cgggttcagt gcctgatact
     7261 gctcaggttc tacggccaga cccagaatgg agtgctcaac gttacgaatc gcaaagaagt
     7321 tcagtgacgt tggcatctgg ctgtacagca cgaagaagat aatggcttcg agcatcagga
     7381 tgaaggcaac gatcatttta cgacgcgcag cacctttcat cgcgaaggct tctttaccga
     7441 agataaccac gataccgaag gcaacaacgc ccagcgccat acgcgcaact tcctgattgt
     7501 gcagcagcca ggtggcgata gcgatcagtg ccacaacacc aataatggtc agcagcaggt
     7561 tacggtagtt gataggctcg aagtctggtt ttgaaccgta ctgtttaacc cagcgttggc
     7621 agaaggcgaa gttaacgata gtgatcagca ggcctacaac gctcaacgca aacgcaacac
     7681 tccagccgta tttcgcggcc agccacggcg tagcaatcat agagaagaaa gagccgatgt
     7741 tgacggacat gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt ttctcatagc
     7801 atgtagaaag cagagaagac gggttagctt taaacaggcc gttaccgacc gcaatagccg
     7861 ccatacccat ataaacgata ccggcgtcgt gaccagacca ggcaaccaga gcataaccaa
     7921 tcgccagcac aatagcgccg agcataatta cgcgtttagt acccagtacc ttgtcaccta
     7981 accagccgcc gatagcgacc agaccataaa ccagggcact aaaggaagag aaaagggtga
     8041 ttgaatccgc ttcagacata cccagttgtt taaccaggta aacagccata attccttgta
     8101 ggccgtaata accaaaacgt tcccataact caatcgagaa gatgagatag aacgccttcg
     8161 gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt ttggtttgca gtggacactt
     8221 ttacctcttt ttttacatcc catattaacg ggggtgcttt ctcgcgttat gtctaaaaaa
     8281 catacgcgca attgttatag tgggagggga aacggcaggt aatgttcact atcttgcact
     8341 ttcagacaag tcctttgtaa taatctgtta catataactg aggcggggaa ttcaaccatt
     8401 ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt aacggcagat tttttcactg
     8461 cactgagagg ggggtgatgg cttaacggcg atatgttctg ctatttagcg tttttactag
     8521 tgagatagtc cagtttctga aaaatagcca gtgtaatgtt ttgtaggtca atatttgttg
     8581 cgtgtgtaaa tttaaatgat tttaacctga ttcttgcaat gtttttacat tgccacagca
     8641 tcgtgatctt gatcacgaat gaatagaaaa tttcgctatt aaaaaaacaa ttaacctgca
     8701 tttttggtta tccgcaaacg gattacctga tgataacgct aagcaatggc atcattgacg
     8761 ttaaaaagat gttttaaaag aggataaaga aaggttatca atggggtaat cggtgttacc
     8821 ccttttcttc agatgtcaac tttctctttg tattcacaaa gatcttcaat aatacaagag
     8881 ccacagcggg gcttgcgggc aatgcaggta taacgcccgt gcaggatcaa ccaatggtgg
     8941 cagtcgactt taaactctgc cggaaccact ttcagtagct tttcttctac ctgttcgaca
     9001 tttttccccg gcgcaaattg agtacgatta caaacgcgga aaatgtgcgt gtcgacagca
     9061 atagtcggcc agccgaatgc agtgtttaat acgacgttgg ctgttttacg acctacgccg
     9121 ggcagggctt caagcgcagc acgatcttcc ggaacctcgc cattatgctg ctccagtaag
     9181 atacggcagg ttttgatgat attttctgct ttgctgttat aaagcccaat cgttttgata
     9241 taggttttaa ccccttcaac gcccagttca agcatcgctg caggcgtatt cgccaccggg
     9301 tagagtttcg ccgtcgcctt attaacactg acatcggtcg cctgagcgga aagcagtacg
     9361 gcaatcagca attcaaaagg cgaactgaaa ttaagctcgg tggtgggatg aggattgttc
     9421 tcacgcaggc gagtgaggat ctccaggcgt tttgctttgt tcatcagaca ttccctgttt
     9481 caccgtttgg caatgcacgt tcggcagctg cttcagtacg gcgctttttc attctttcat
     9541 caatcaggta ttttcctgcc agcatcagtc ccaggccaat aaatgcacct ggaggcagca
     9601 tcgccagcag gaaaggggag tcggtgtgga aaatttccac gcgtaatact tttgcccagc
     9661 tacctaacag cgcatctgca ccgtcaaaca atgtgccatt gccgataatt tcgcgtagtg
     9721 aacccagcac gaacatggcg caggttgcgc ccataccaat tgaaaagccg tccagtgccg
     9781 aaagcgccgg accttttttg gcggcgaagg cttcagcgcg gcccacaacg atacagttag
     9841 tgacaatcag cggaataaaa atccctaatg attgatacag gccaaaggcg taggcgttga
     9901 tcagcatctg tacagcgctg accaccgagg cgatgatcat cacgtaaatg ggaatgcgga
     9961 tctcggctgg cgtccagtga cgcagcgtcg aaatggtcag gttggtcagc gtcagtacaa
    10021 gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt ggacgtgacc gccaacagag
    10081 gacaaaggcc gagcaactgg accagcgcag agttgttttt ccacaacccc tgaacaataa
    10141 cgtctttaat ttcgctcacg ctttattctc cacaggcagg aagttgagaa agttgtgccg
    10201 gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt aaccaccgcg cggggagtaa
    10261 tcgtcgcgcc ggtgaactgg tcgaaatcac caccatcttt cttcaccgcc cagtgcgcat
    10321 catctgcacc actgattttt ttacccgcaa aatgggtgat ccagtcagaa aggcgcagtt
    10381 cgattttatc gccaagccct ggcgtttcgt ggtgctctgt cacgcgcgtg ccaagtaccg
    10441 tgccgttaaa atcggctccc accagcagct gaatcgcacc ggaatagcca tctggcgcgg
    10501 ttgcttccag aacggcggct accggtttgt catcctgttt ggcgatgtaa acccgatgct
    10561 cacctttacc taactctggc gcagttacca gatagcaact ctgtgccagc gcattgttat
    10621 agcgttcggc tggcagcacc tgatcaaata atgccttttg ttgcagactg gcctgttcag
    10681 caatcgtcgt tttggtcatc tggttgatgg ccgcagttaa ccctgttgaa cccgctgcaa
    10741 atagcgctaa cgtaatgccg tgttttcgga tagttttcag catggtttac cctttgcgat
    10801 ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg aaccgtgatg ttcgccagca
    10861 ggacggcaaa agccacgccg tcaggatagc cgccgaaact gcggatcaac cagactaata
    10921 agcccgcaag cgcgccgaaa ataagacgac cacgattggt cgtagaagcg gtaaccgggt
    10981 cagtcaaaat aaagaatgcg ccgagcatgg tcgcaccaga cagcagatga atttgcggtg
    11041 ctgccagtgt ttctggtgag aacaaccagc ccaacgttgc gcataacgcc agcgttacta
    11101 agaagctgag gggaatatgc cagcgaatcg ctttctgcca tagcaaccac aggccgccag
    11161 ccagccaggc gagatttacc cattgccaac cagcgcccgc cagaataccg ctgtagatcg
    11221 gatattgcat aatctgttca accgaatgac cggcacggac agaggtttta aatgtatcca
    11281 gcggtgtcgc ctgactaatg ccatcaatac ctaagcgtag tgtgttcata tcaccaccac
    11341 tggcggtatg cccgctgaaa ataacctgta tggcgtcgat aaaaccaggg atgttgaccg
    11401 caatttcatg tggcggtaac cagctggtca tctgcaccgg gaaggagatc agtaagacca
    11461 cataaccaat cattgctgga ttaaacgggt tttgccccag accgccatac aactgtttag
    11521 cgatgatcac cgcaaacacc gtacccagca cgaccatcca ccatggcgcg agggggggaa
    11581 tacttaccgc cagcaataag cctgtcagca atgctgagtt atctttcaac gttgcggcta
    11641 ccgactgctt gcgtagtttg agtacgagag cttcggctaa cagagcacta accgatgcca
    11701 acaggatctg aacgagagta ccccaaccaa aaaaccacag ttgcgctgcg attcctggca
    11761 cggctgcgag caacaccaac agcataatgc gcgatgtctg gcgctggtta tgggtataag
    11821 gggagctagc tattctgaat accatttagt cctcgtttac aaccttctgc tgggcggctt
    11881 ttttggcctg agcgcgggca atagccgcgg caactgccgc tttgcgcgga tcaacctgtt
    11941 cttctggttc tgcgttagtc tgttgctgtt ccagcttgcg cgctttggca cgggcaatag
    12001 cggcttcgac ggcggctttg cgcggatcaa cctgttcttc tggttccgca ttagcctgtt
    12061 gctgttccag tttgcgcgct ttggctcggg caatagcggc ttcgacggcg gctt
//
LOCUS       DAMZII010000145        12089 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZII010000145 DAMZII010000000
VERSION     DAMZII010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.145"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            102..704
                     /locus_tag="POA77_003957"
     CDS             102..704
                     /locus_tag="POA77_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDI6367318.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(706..1227)
                     /gene="ruvC"
                     /locus_tag="POA77_003958"
     CDS             complement(706..1227)
                     /gene="ruvC"
                     /locus_tag="POA77_003958"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDI6367319.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1262..2002)
                     /locus_tag="POA77_003959"
     CDS             complement(1262..2002)
                     /locus_tag="POA77_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6367320.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2031..2483)
                     /gene="nudB"
                     /locus_tag="POA77_003960"
     CDS             complement(2031..2483)
                     /gene="nudB"
                     /locus_tag="POA77_003960"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDI6367321.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2601..4373)
                     /gene="aspS"
                     /locus_tag="POA77_003961"
     CDS             complement(2601..4373)
                     /gene="aspS"
                     /locus_tag="POA77_003961"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6367322.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4683..5249
                     /locus_tag="POA77_003962"
     CDS             4683..5249
                     /locus_tag="POA77_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6367323.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5246..6064
                     /locus_tag="POA77_003963"
     CDS             5246..6064
                     /locus_tag="POA77_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI6367324.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6117..6512
                     /locus_tag="POA77_003964"
     CDS             6117..6512
                     /locus_tag="POA77_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDI6367325.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6553..7296
                     /gene="cmoA"
                     /locus_tag="POA77_003965"
     CDS             6553..7296
                     /gene="cmoA"
                     /locus_tag="POA77_003965"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDI6367326.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7293..8264
                     /gene="cmoB"
                     /locus_tag="POA77_003966"
     CDS             7293..8264
                     /gene="cmoB"
                     /locus_tag="POA77_003966"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDI6367327.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLIPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8429..10858)
                     /gene="torZ"
                     /locus_tag="POA77_003967"
     CDS             complement(8429..10858)
                     /gene="torZ"
                     /locus_tag="POA77_003967"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDI6367328.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRSSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDATRFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10883..11983)
                     /locus_tag="POA77_003968"
     CDS             complement(10883..11983)
                     /locus_tag="POA77_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDI6367329.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2783 a         3219 c         2921 g         3166 t
ORIGIN      
        1 cagtgaagaa atgcgtaata aaaatataca ttgcgactcc tgaaaaaata aattttttat
       61 gctattacgt atattcgtat cgatttcaat ggaatgacaa cgtgaatatt aattaccctg
      121 ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta tttggtcaaa
      181 tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt cataacggtg
      241 aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg ctatctcgtt
      301 ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct gggctgacag
      361 aacaacaaaa acaacgaatt gttgaacagg ttccttcccg gctacgcaaa ctttaccaca
      421 cgggttttaa atacgaatct tcccgccagt tctgttcaaa atttgtcttt gatatttata
      481 aagaggcgct atgtattccg gtgggtgaaa tagagacgtt tggacaactg ttaaatagca
      541 atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt ccgtgggagc
      601 gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tttggtgttg attcacgcgg
      661 agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac gcagtcgccc
      721 tctcgccagg ttcagccgcg attcgctcat ctgcatcgca ttctgactga cgtgacaatg
      781 ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg gcagtttcag
      841 caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac ctaccaccgt
      901 ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca ccgccgcaac
      961 aatcgccacg ccgcgcgcct ggcccagttt cagggctgag tcagcgttct tcgccataaa
     1021 gacttgttca atggcgaaat aatcaggctg gaactgggtg atgatttccg tcacgcccgc
     1081 atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc atccgctacc
     1141 caggtaggac agttgcctgc ctacctggcg aattacgcca tagccggtca cgcgcgaacc
     1201 cggatcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt agcaggcctc
     1261 atcagagagt cgctgcgacc tcatcagaga tttcaccgtt atggtaaact tcctgcacgt
     1321 cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt tctgcatcca
     1381 tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc agacctgccg
     1441 cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagaca tcaatcgcgc
     1501 cgtcatcata ggtcacaacg tcttcagcac ctgcttccag agctgcttcc atgatggtgt
     1561 cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag gctacggaac
     1621 catctgtacc gaggttaccg ccacatttgc tgaatgcatg acgcacttca gcaacggtac
     1681 ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga ccgtaacctt
     1741 cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt gcaattgcgc
     1801 ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc gcacgcagac
     1861 gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc tcacgaatga
     1921 ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt ctggtgttgg
     1981 cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt tcaggcagcg
     2041 ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag cgccgccgca
     2101 gcaggcgcat caagccactt gtaagccaga tgttcagtga aaacgatctg cctctcgtgc
     2161 ggaagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc atagcgatga
     2221 cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa ggtcagttgt
     2281 tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc ttgcggcgcg
     2341 gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc gtcacgccgc
     2401 tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat cgaaacggga
     2461 cgcttataca ctttatcctc cacgctgcct cttcgttgac tgccttcgct catcccattc
     2521 acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca acgcgaatga
     2581 tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg aatgctcagc
     2641 tcagccagtg cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca cgctgccgcc
     2701 gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccggtcag cagcatggtc
     2761 agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt cagagcgtcg
     2821 agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat accaaacacc
     2881 gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc gtaaccattg
     2941 atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag ttctgcagca
     3001 gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc ttcaccgtcg
     3061 tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc gtcggtcaga
     3121 ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac aattttcttg
     3181 ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc caggatggct
     3241 tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc cagacctttc
     3301 gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt aacgaagtta
     3361 ccgtattcgt cgatctgctt acgggtcagc gatgcgccgc ccggaacgcg cagagcggca
     3421 acgcgacctt tcggatcgtt cgccggacct gcaaatactg caaactcaac agatttcagc
     3481 agatcagcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc agaaccataa
     3541 cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac gcccttcact
     3601 tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg cgcggtcatg
     3661 aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg caggtcttcg
     3721 tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat cagcagctgt
     3781 ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac acgagaaggc
     3841 accaggtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt ttcgatgtcg
     3901 aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc gcgggttttc
     3961 aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag acgcgcttct
     4021 tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat gatagtcagc
     4081 gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt tttttcgtca
     4141 cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc agaggccagc
     4201 tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc gcggtcgcgc
     4261 atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc acacagagtc
     4321 acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg catgagatat
     4381 cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt cgcagcgtta
     4441 gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc tgccgcaccg
     4501 taagagcctg gcccgcgctg gaacgcctcg ttaccacttt atatcgggcc tgaaatcaga
     4561 ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac aaaatttgtc
     4621 gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt tcaggagtca
     4681 gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta caagaaggca
     4741 tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc gccgggaagc
     4801 tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgcgtt ggctggtctg
     4861 ccgatcacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct aaagtgttgc
     4921 ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat atcgaaatca
     4981 tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg cgccgccgag
     5041 gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa tccaccgccc
     5101 gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt agtgccgcca
     5161 gcgccgagca gcacaataac agcatcaacc atatctaccc acgcatcgcc cgtgtgcgta
     5221 gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat ggtcgcatcc
     5281 taaatgggtg cggttgggaa tcaccagcct tgaagagtat gcccgccact ttaactgcgt
     5341 ggagggcaac accacgcttt acgccctgcc gaaacccgag gttgtcctgc gctggcgtga
     5401 acagaccaca gatgacttcc gcttctgttt taagtttccg gcgaccattt cgcatcaggc
     5461 agcattacga cattgcgatg atttagtgac tgaatttttg acccgcatgt caccgttggc
     5521 tccgcgcatt gggcaatact ggctgcaact gcctgccaca ttcggcccac gggagctgcc
     5581 tgcgctttgg cattttctcg attctctccc tggcgaattt aattatggtg tggaagtccg
     5641 ccatccacag tttttcgcca aaggggaaga ggaacaaacg cttaatcgcg gtttacatca
     5701 gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt catgcagcac gtccacacag
     5761 tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac
     5821 ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa accgggaatt
     5881 atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc cttatctttt
     5941 tttacatacg ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt
     6001 acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt
     6061 ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg
     6121 taagcgcgct gtatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg
     6181 ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa
     6241 gcgctattcg cattcatggt aacgcggtgg aatatattcc catcgcgatt gtgctgatgc
     6301 tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc
     6361 ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgttccgc tggcgacgtt
     6421 ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata
     6481 tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg
     6541 gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc
     6601 gactggacct ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt
     6661 cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct
     6721 ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc
     6781 aacattcatc atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa
     6841 cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt
     6901 gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa
     6961 ttcctggaac cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg
     7021 ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg
     7081 ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc
     7141 cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca
     7201 cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt
     7261 tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc
     7321 tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact
     7381 ggcagcgcga gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc
     7441 ctgaaattaa accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc
     7501 cactgagcac cgggcaaatt aaacgcattg aaacgcttat gcggaacctg atgccatggc
     7561 gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga
     7621 aatgggatcg tgttctgccc catctttctg atttaaccgg acgcaccatt ctggatgtcg
     7681 gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg
     7741 ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta
     7801 acgatcagcg cgcacatttg ataccgttag gtattgaaca gcttccggca ctgaaagcct
     7861 ttgataccgt cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct
     7921 ggcagttgaa agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg
     7981 atggtgatga gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct
     8041 atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata
     8101 tccgcattgt ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg
     8161 ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt
     8221 atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg
     8281 ccggatgcgg cgtgaatgcc ttatccggcc tacaaaccct tgctaattca atctattgca
     8341 gggactatgt aggcctgata agcatagcgc atcaggcagt tttacgtttg cataacctca
     8401 gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg
     8461 ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac
     8521 aggcatttgc cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt
     8581 tacataagcc attttccaga tctggccatg caccttcatg cacgcatacc acgccttttt
     8641 tgatcccgtc agtcaccacc gcacctgtta gtatctgacc acgtttgttc cagacacgca
     8701 ccagatcgcc attcgcaata ccaaatcgag tagcatcttc ggtgtgaata gtgatcggtt
     8761 cacgatcggc aaccgcatat tttttacgca gtcccgcata gttaagctga ctgtgtaaac
     8821 ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact
     8881 catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg
     8941 ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc accggatcgg
     9001 cgcggaaatc accataacga acgtactgtt cgttcttctc gctgctgcgc atttcgatca
     9061 gtttattttg ctgccagaag gcattaaaca tcggcatagt cacgcgttgc gcacgagcac
     9121 ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat
     9181 agatctcttt tccgccaggt ttcagtaatt cagcaagatc ggcaaatacg tcaaaatcgt
     9241 tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt
     9301 aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat
     9361 ctgcatgttt agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct
     9421 gccatgcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc
     9481 accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga tatttgccac
     9541 caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt ccgccatcat
     9601 cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa
     9661 cacgcgtcgg attgccaccg ttggagtaat gataagagaa accaaaaccg ccgcccggtg
     9721 taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat
     9781 attgctggcg ctgaattccc cagccagcca tcagcattgt gcgattcgcc gccatcagtt
     9841 cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc
     9901 tcttcggagt attatcgctc ttacctgtca gatactcttc aaactgcgga tacccggtgg
     9961 tgtatttttc aaggaacact ttatcgtgtt taccttgtgt catcagggta tgcgcaatcc
    10021 ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcaccaa
    10081 agaactcgat ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt
    10141 tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg
    10201 tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga atcagcggcc
    10261 aggaggtctg ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg
    10321 ccgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac
    10381 gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg
    10441 caaaaatagc cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt
    10501 ttaatgcttg ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt
    10561 gcagtggatt atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt
    10621 gtacctgatc cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg
    10681 ttgaagaaac aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg
    10741 tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc gatgtcacca
    10801 ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt aatgtcataa
    10861 cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga
    10921 aataccgcgt taaaatgtcc agttcgtttt cgctcatgct ggttcgcgca cccattcctt
    10981 tggcaatgga cggccatgca ttgacggtgt aatggtcagc ggcaataggc gcatggcaac
    11041 cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta cggtccgcta
    11101 atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt
    11161 cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt aatgtggcta
    11221 gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc atttgataac
    11281 cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga tcggtaccag
    11341 gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata tgtgcgccat
    11401 tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta
    11461 tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg ccgccctttt
    11521 gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc atggcatcaa
    11581 aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc catactgttt
    11641 cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg ccgctaacaa
    11701 attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact
    11761 ttggaatatg gcaatcggca cattccgcac gtatcccttt ctggttcgaa aagtggacag
    11821 ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgctgtat
    11881 ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg
    11941 ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaaataaa
    12001 ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg cttgcataac
    12061 tcggcacaac actgcctgaa ataatcgat
//
LOCUS       DAMZII010000146        12082 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZII010000146 DAMZII010000000
VERSION     DAMZII010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.146"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..139
                     /locus_tag="POA77_003969"
     CDS             <1..139
                     /locus_tag="POA77_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDI6367330.1"
                     /translation="NIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWP
                     F"
     gene            463..1851
                     /gene="narU"
                     /locus_tag="POA77_003970"
     CDS             463..1851
                     /gene="narU"
                     /locus_tag="POA77_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDI6367331.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1933..5673
                     /gene="narZ"
                     /locus_tag="POA77_003971"
     CDS             1933..5673
                     /gene="narZ"
                     /locus_tag="POA77_003971"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDI6367332.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5670..7214
                     /gene="narH"
                     /locus_tag="POA77_003972"
     CDS             5670..7214
                     /gene="narH"
                     /locus_tag="POA77_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6367333.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7214..7909
                     /gene="narW"
                     /locus_tag="POA77_003973"
     CDS             7214..7909
                     /gene="narW"
                     /locus_tag="POA77_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6367334.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7906..8586
                     /gene="narI"
                     /locus_tag="POA77_003974"
     CDS             7906..8586
                     /gene="narI"
                     /locus_tag="POA77_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6367335.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8666..9559
                     /locus_tag="POA77_003975"
     CDS             8666..9559
                     /locus_tag="POA77_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDI6367336.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGVWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9655..10500)
                     /gene="nhoA"
                     /locus_tag="POA77_003976"
     CDS             complement(9655..10500)
                     /gene="nhoA"
                     /locus_tag="POA77_003976"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDI6367337.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10673..11242
                     /locus_tag="POA77_003977"
     CDS             10673..11242
                     /locus_tag="POA77_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6367338.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIAVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11246..11473)
                     /gene="pptA"
                     /locus_tag="POA77_003978"
     CDS             complement(11246..11473)
                     /gene="pptA"
                     /locus_tag="POA77_003978"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDI6367339.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11524..12082)
                     /locus_tag="POA77_003979"
                     /pseudo
     CDS             complement(11524..12082)
                     /locus_tag="POA77_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2887 a         3113 c         3123 g         2959 t
ORIGIN      
        1 gaatataaaa gttaaaaaca tgaccttaga tgaaataaac aatacttact gtaataacga
       61 ttattacaat caggcaataa gagaggaacc gatagacctt ctggacagat cgttttcctc
      121 cagttcatgg cctttttagt gaataaatca tcactaattc tataagtgtc tggtcttgtt
      181 gataatatag ttaatatgta accctttaga ttcacgatct gaagggttga aacatgacat
      241 acgttcaaag attaaccccc gtttgaaggt tagcttgtta gttacattta gtaacacata
      301 tctggctact taatccccat tctgcccccg cggttacgct ttcttatcta ttctttcaat
      361 cagcacttca cccaattcat ccttaattct aataactggc tattgcgcct tacctcctgt
      421 tttgtctttt ttcattacca atgtgtgcat gtgaggaaca atatggcact gcaaaatgag
      481 aaaaatagtc gttatctttt acgcgactgg aaaccagaaa atccggcctt ctgggaaaat
      541 aaaggaaaac atattgctcg aagaaacctc tggatatcag tcagttgtct acttcttgcc
      601 ttctgtgtct ggatgctatt tagcgcagtt accgttaatc tcaataaaat cggttttaat
      661 tttactaccg atcaactctt tttattaacc gcattaccct ccgtttctgg cgcattattg
      721 cgtgttccct actcctttat ggtgcctata ttcggtggac gccgatggac ggtttttagt
      781 actgcaatcc tgattattcc ttgcgtctgg ctcggaattg ccgtgcaaaa tccgaatact
      841 ccttttggga tatttatcgt tatcgctttg ctatgcggtt ttgcaggtgc aaactttgct
      901 tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca aacaagggag cgctcttggg
      961 attaatggcg gattaggaaa cttaggtgta agtgtaatgc agctggttgc accgctggtc
     1021 atttttgtac ctgtatttgc ctttctcggc gtcaatggcg taccgcaggc cgacggttcg
     1081 gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc tactggcgat tgccacgatc
     1141 gccgcatggt cagggatgaa tgatatcgcc agttcacgcg cctcaattgc cgaccagctc
     1201 cctgtcttac aacgcctgca tctctggctg ctgagcctgc tttaccttgc caccttcggt
     1261 tcgtttatcg gtttttctgc gggttttgcc atgctggcaa aaacccagtt cccggatgtg
     1321 aatattctgc gcctggcgtt ctttggccca tttatcggtg ccatcgcgcg gtcggttggt
     1381 ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt tgatcaactt tatttttatg
     1441 gcgattttca gtgccctgct gttccttacc ttaccgggca caggctccgg taatttcatc
     1501 gccttttacg ccgtatttat ggggctgttt ctgactgcgg gtctgggaag tggttctact
     1561 ttccagatga tcgccgtcat ctttcgccag ataaccattt atcgggtaaa gatgaaaggc
     1621 ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa cggcggcggc tctgggcttt
     1681 atctcagcca ttggcgcagt gggcggcttt tttattccgc aggcgtttgg catgtcgctc
     1741 aatatgaccg gctctccggt cggcgcgatg aaagtgtttt taatcttcta catcgtttgt
     1801 gtgctgctga cctggctggt ttatggtcgg cggaagttca gccaaaaata agagatcgat
     1861 gcctgatgta caaccgtgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc
     1921 aggagtcatg tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa
     1981 acctttgccg atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat
     2041 cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc acggtgttaa ctgtacaggc
     2101 tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc
     2161 gactacccgc gcactcgccc tgacctgccc aatcatgaac ctcgcggctg cccgcgtggc
     2221 gcaagttact cctggtatct ttacagcgct aaccgcctga aatacccgct cattcgtaaa
     2281 cgactgattg aactgtggcg cgaagccctc aagcaacaca gcgatccggt actggcgtgg
     2341 gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca aacaagtgcg tgggcgcggc
     2401 gggtttatcc gctccaactg gcaggaacta aaccagctga ttgccgccgc taacgtctgg
     2461 accatcaaaa cctacggccc ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg
     2521 atggtttctt acgccgccgg aacgcgttat ctgtcactgc ttggcggcac ctgtttaagc
     2581 ttctacgact ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc
     2641 gacgtaccgg aatctgccga ctggtataac tccagctaca tcatcgcctg ggggtctaac
     2701 gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg aagtacgcta caaaggcact
     2761 aaaaccatcg ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg
     2821 gcaccgaaac aaggcactga tagcgccctg gcgatggcaa tgggccatgt gattttaaaa
     2881 gagtttcatc tcgataatcc cagcgactac tttatcaact actgccgccg ctacagcgac
     2941 atgccgatgc tggtaatgct ggagcctcgc gacgatggta gctacgttcc cgggcgcatg
     3001 atccgcgcat ctgacctggt ggatggactg ggcgaaagca acaatccgca gtggaaaacc
     3061 gtagcagtta ataccgcagg tgaattggta gtgccgaacg gttcgattgg tttccgctgg
     3121 ggagaaaaag gcaaatggaa tctggaatcc attgccgccg gtacggaaac cgaattgtcg
     3181 ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg ccttccccta ctttggcggc
     3241 attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg tgctggtgcg ccaattgccc
     3301 gttaaaaacc tgacgttagt cgatggcaac acctgtccgg tggtcagcgt ttatgatttg
     3361 gtactggcga attacggcct cgatcgcggg ctggaagatg aaaacagtgc gaaagattac
     3421 gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt gccgcgccag
     3481 tatattgaaa ccatcgcccg tgaatttgcc gataccgccc ataaaacgca tgggcgctcg
     3541 atgattatcc tcggcgcagg tgttaaccac tggtatcaca tggacatgaa ctaccgcggg
     3601 atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg ctgggcacac
     3661 tatgtcggcc aggaaaaact gcgcccacaa accggctggt tgccactggc ctttgcgctc
     3721 gactggaacc gaccaccgcg ccaaatgaac agcacctcgt ttttctacaa tcattccagc
     3781 caatggcgct atgaaaaagt ctctgctcag gagttacttt caccgctcgc cgatgccagt
     3841 aagtacagcg gtcatctgat tgatttcaac gttcgcgccg aacgtatggg ctggctacct
     3901 tctgcgccgc agctggggcg taacccgctc gggattaaag ctgaagccga caaggccgga
     3961 ttatccccca cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc
     4021 gaacaaccag atagcagcag caatcatccg cgtaatttgt ttgtctggcg ctctaacctg
     4081 cttggctcct ccggcaaagg ccacgagtat atgcagaagt atctgctggg gaccgaaagc
     4141 gggattcagg gcgaggaact cggtgccagc gacgggatca aaccggaaga agtcgagtgg
     4201 caaactgcag cgattgaagg caagctcgac ctgctggtga cgctcgactt ccgcatgtcc
     4261 agtacctgcc tgttctccga tatcgttctg cccaccgcca cctggtacga aaaagacgat
     4321 atgaacacct cggatatgca tccgtttatt catccgcttt ctgcggcggt cgatccggcg
     4381 tgggaatcac gcagcgactg ggaaatctac aaaggtatcg ccaaagcatt ttcgcaagtg
     4441 tgcgtgggtc atcttggcaa agaaaccgac gtggtattac aacccctact gcatgactct
     4501 ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc gcaaaggcga atgcgatctg
     4561 attccaggca aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacgtat
     4621 gaacgcttta cctcgctcgg gccattgatg gacaaacttg gcaatggcgg taaagggatt
     4681 tcgtggaata cccaggatga aatcgatttc ctcggcaaac tcaattacac caagcgtgat
     4741 ggcccggcac aggggcgtcc gctgattgac accgccattg acgcttcaga agtgattctg
     4801 gcactggcac cggaaactaa cggtcatgtt gcagtcaaag cgtggcaggc gctgggcgag
     4861 atcaccgggc gcgaacatac ccatctggcg ctgcacaaag aggacgagaa gattcgcttt
     4921 cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc ccacctggtc cggtctggaa
     4981 agcgatcacg tatcctataa cgcgggatac accaacgttc atgagttaat tccgtggcgc
     5041 acgctgtcgg gacgccagca gctctatcag gatcatccgt ggatgcgtgc ttttggtgaa
     5101 agcctggtgg cttatcgccc gcctatcgac acccgtagcg tcagtgagat gcgccagata
     5161 ccgccaaacg gcttcccgga aaaagcactt aacttcctga cgccgcacca gaaatggggc
     5221 attcactcaa cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt
     5281 gtctggatca gcgaaacaga tgcccgagaa ctgaccattg tcgataacga ctgggtggaa
     5341 gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg tcagccaacg tgtaccgccg
     5401 ggcatgacca tgatgtatca cgcccaggaa cgcattatga atattcctgg ttcggaagta
     5461 actggcatgc gcggcggcat tcataactcg gtcacccgcg tttgcccgaa accaacgcat
     5521 atgattggcg gttacgcgca gctggcctgg ggctttaact actacggcac cgtcggatcg
     5581 aaccgcgatg agttcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa
     5641 ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg ttcacaagtc ggcatggtgc
     5701 ttaacctcga caaatgtatc ggctgccata cctgttcggt gacctgtaaa aacgtctgga
     5761 ccgggcgcga aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg
     5821 gttatccgaa aaactgggaa gatcaggaag agtggcaagg cggctgggtc cgcgatgtga
     5881 atggcaagat ccgtccgcgt ctgggcagca agatgggcgt aataaccaaa atcttcgcca
     5941 acccggtggt gccgcagatt gatgattact acgaaccttt caccttcgac tacgaacatt
     6001 tgcatagcgc accggaaggc aaacatattc ctactgctcg cccgcgttca ctgattgacg
     6061 gcaaacggat ggacaaagtg atctggggcc caaactggga agaactgctg ggcggcgagt
     6121 ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca aaaggagatg tacgggcagt
     6181 ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga acactgcctc aatcccagtt
     6241 gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga agaagacggc attgtgctga
     6301 ttgatcagga taaatgccgt ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa
     6361 tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat cttctgttat ccgcgaattg
     6421 agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg tcgcatccgg tatctgggcg
     6481 tgctgcttta cgacgccgac cgcattgagg aagcggcgag caccgagcgc gaagttgacc
     6541 tctatgaacg tcagtgcgaa gtattcctcg atccacacga tccctcagtg atcgaggaag
     6601 ccctgaaaca aggtattcca caaaacgtga ttgaagctgc ccagcgttcg cctgtctaca
     6661 aaatggcgat ggactggaaa ctggcgctac cgctgcaccc tgaatatcgc accctgccaa
     6721 tggtctggta cgttcctccg ctgtcgccga ttcagtccta cgcagatgcg ggcggtttgc
     6781 cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg tattccggtg caatatctcg
     6841 ccaatatgtt gagtgccggc gataccggtc cggtactgcg ggcgctgaaa cggatgatgg
     6901 cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt tactgatact cgtgccatcg
     6961 acgaagtagg cctgagcgtc gcccaggtcg aagagatgta tcgttacctc gccattgcca
     7021 actatgaaga tcgttttgtc atcccgacga gccatcggga aatggcgggc gatgccttcg
     7081 cagaacgcaa cggctgcggt tttacctttg gcgacggttg ccacggttcg gacagtaaat
     7141 tcaacctgtt caacagtagc cgtatcgatg ccatcaacat caccgaagtg cgcgacaaag
     7201 cggagggcga ataatgcaga tcctcaaagt gatcggcctg ttgatggagt atccggatga
     7261 gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc cgccgcgacg cgccgatgct
     7321 tacggatttc acccacaacc tgcttaacgc gccgctgctg gataaacagg ccgaatggtg
     7381 cgaagtgttt gaccgcgggc gcaccacgtc gctgctgctg ttcgaacatg ttcatgccga
     7441 gtcccgcgat cgcggccagg caatggtgga cctgctggcg gagtatgaaa aggtcggcct
     7501 gcagctggat tgtcgggaac tgcccgatta tctaccgctg tatctggagt atttaagcgt
     7561 actgcctgac gatcaggcga aagaaggatt gctcaatgtt gcgccgatcc tcgccctgct
     7621 tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg ttgtttgatg ctctgctgca
     7681 actggcggga agtatacttt caagtgacag tgtcacaaag caagtaaaca gcgaagagcg
     7741 cgatgacacc cgccaggcgc ttgatgcagt gtgggaagag gaacaggtga agtttattga
     7801 agataacgcc acggcgtgtg acagctcgcc gcttaatcaa tatcagcgac gctttagcca
     7861 ggatgtcgcg ccgcagtatg tcgacatcag tgcgggaggt gggaaatgat tcagtatctg
     7921 aacgtctttt tttacgatat ctacccgtac atttgtgcga cggtgttttt cctcggcagc
     7981 tggctgcgct acgactacgg gcagtacacc tggcgcgcct cctcaagtca aatgctcgat
     8041 aaacgcggga tggtgatatg gtcgaatctg ttccatatcg gcattttggg gattttcttc
     8101 gggcacttgt tcggcatgtt aacgccgcac tggatgtacg cgtggttttt gccagtggca
     8161 gcgaaacaac tcatggcgat ggtgctcggc ggtatttgcg gtgtgttgac gttgattggc
     8221 ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg tgcgtgcaac ttccactacg
     8281 ccggatatta tcatcatgag cattttgctg atccagtgtc tgctggggct aagtaccata
     8341 ccgttttcag cacagtatcc tgatggtagc gaaatgatga agctggtggg ttgggcgcaa
     8401 agcattgtca ctttccgtgg tggatcgtca gaaatgctta acggcgtagc gttcgttttc
     8461 cgcctgcatc tggtattggg aatgaccatc ttcctgctct tcccgttcac cagattggtg
     8521 catgtgtgga gcgcgccgtt tgagtacttt actcgtcggt atcagattgt gcgttcgcgg
     8581 cgataatttt caccctggcc ggatagccct tcgctctccg gccacttgca ggctatgata
     8641 gttctccagc cacaggagaa cgttaatgaa accgcaggtg taccacgtcg atgcttttac
     8701 ctcacaaccg tttcgcggca attctgccgg tgttgttttc cccgccgata atcttagcga
     8761 agcgcaaatg cagcttatcg cccgcgagtt aggccattcg gaaactgctt ttctgctgca
     8821 cagcgacgac agcgatgtgc gcatccgcta ctttacgcca acggttgaag tgccgatttg
     8881 cggtcacgcg acagtagctg cgcactatgt acgtgcgaag gtgttaggtt taggaaattg
     8941 cacgatctgg caaacatcgc tggcaggaaa acatcgcgtg actatcgaaa agcacaacga
     9001 tgattatcgt atttcgctgg aacaaggtac gccgggcttt gagccaccgc tggaaggtga
     9061 aacacgtgag gcgattatca acgcgcttca tctcactgaa gacgatattt tgccgggctt
     9121 gccaattcag gtcgcaacaa caggacactc aaaagtgatg atcccgttga aaccggaagt
     9181 ggatatcgac gccctttcgc ctgatcttgc tgcgctgagc gctatcagca aacagattgg
     9241 ctgcaatggt ttcttcccgt tccagatccg cccaggcaaa aacgaaaccg atggtcgtat
     9301 gttctcgcct gcgattggta ttgtggaaga tccggtgact ggcaatgcca atggcccgat
     9361 gggtgtatgg ttggtacatc acaacgtatt gccccacgat ggcaacgtgt tatgtgttaa
     9421 aggccatcag gggcgcgcat tggggcgtga cggcatgatt gaagtgacgg tgacaattcg
     9481 tgataaccaa ccggaaaaag tcaccatttc aggcacagcg gtgattttgt ttcatgccga
     9541 atgggcaatt gaactctgaa accgttgcct gatgcgcttc gcctgtcagg tctacaaccg
     9601 agctaatgta tgccggataa ggcgtttacg ccgcatccgg caacctgaca taaattattt
     9661 tcccgcctcc ggatgcgtat caaacgccgc catcaccagc gctaactcat ccacagtaaa
     9721 accatgtttc gcatcatcca cgcccagacc aaactgttcc tgcatcacgg catataacga
     9781 caccacatcc ggtagatttc gctgctccac cgcgtgccca ttttcataat gggtaaaatg
     9841 aaaattggtc agcgttagct tgccaccgtc cggcaaatgg cggcacatca gcaaatgatg
     9901 gcgaaaatgc gactgcggcc agtgcgccga ccagaaattg cccatcacat aatcgctttg
     9961 ttgctgctcg cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg
    10021 caacacccaa tcatcaccct cctgcaacaa ccgataatct ccgtgtggcg tggtctgcac
    10081 gagatcggaa actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc
    10141 aatccatttt tcctcttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc
    10201 tggcggattt gataacacta cgcgccctaa caagctgcga acgttaaact ccagctcgcg
    10261 taacacccgc tcaaacacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac
    10321 cagtttctct tccagcgatt gatcatcaag ctgtatttcc ctcggcagca aaacgtcgag
    10381 gttttcaaac ggaatagtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt
    10441 gaccgcagca gctcccgacc agttaatacg ggcaaaatag tgattcagaa tgggcgtcat
    10501 caggcgtttc ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta
    10561 ttttgtgcga tcgcggacat gcatcacgtt tgcaaatcgc caatcccgtt aatttcgtta
    10621 cttattgcgt tacggactac gcttaaaaaa accttcttcg cgaggcgata ccatgagccg
    10681 attcatcccc atagaattac accatgccag ccgtctgtta aatcatggcc caactgtcat
    10741 gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt atggcagcag cctggtcaat
    10801 gccggtggag tttgaaccgc cacgcgtggc gattgcggta gataaatcga catggaccag
    10861 ggagttgatt gaacgtaacg gtaaatttgg catcgttatc ccgggcgttg cagcaactaa
    10921 ctggacgtgg gcagtgggaa gcgtgtcggg tcgtgaagaa gataaattta attgctatgg
    10981 cattccggtg gtgaaaggtc cggtgcttgg tttgccactt gttgaagaga aatgcctggc
    11041 gtggatggag tgccgcctgc tacctgcgac ttctgcgcaa caaaaatacg acacgctgtt
    11101 tggcgaggtg gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt
    11161 tgatgatgat aagctcaata cgttgcatca tttaggtgct ggcacgtttg ttaccagcgg
    11221 caagcgagtt acggcgggtt aagcattaca tgctgtaacc gggtttcttt atcaaagcct
    11281 ccatttgggg cgcgatttcg gcatcccaga tagcttgcca agattctggt tgaatctgct
    11341 gtagagcaat gcttatcgag ctatctttac tgttcagatg gcgaataata acgtcggtaa
    11401 tatctgcagc aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa catttaatgt
    11461 cgatgtgcgg catgattgtg ctccttttga aagatgtcag taaaattaac acacattgtg
    11521 aattttcagt cctttccatt caaacgtgaa atcaataagt tcatcaggga catcgcaaac
    11581 aatatgaata cggatttctt ctctgccgtg actcttttca caaattgcgt aactgtcatg
    11641 ctctggatta tttaattctt tcagcggaaa tttttcctca aaggctttat ttagccaccc
    11701 ctggtttcct ttcaccgcga ataaataatc acctccctgt ttttgtatct tctctgctat
    11761 atctttctgg caacccatcg catcagttgt gatgattttt cctttaatat ccagcatgtt
    11821 aagaagttct gggatagctg taatctcatt agatttatca tccgtcttga tctgtccgat
    11881 gaccagactg tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact
    11941 cttatcataa gaatgccgga gcgtttttcc atcaattgca atgacgtctt tatcatctga
    12001 agaatgacaa tcacgcatcc agttaataaa gcactcatga aattttgtag ggctgataca
    12061 ggatacaact ctggcaatag ta
//
LOCUS       DAMZII010000147        11939 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMZII010000147 DAMZII010000000
VERSION     DAMZII010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.147"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            68..183
                     /gene="rrf"
                     /locus_tag="POA77_003980"
     rRNA            68..183
                     /gene="rrf"
                     /locus_tag="POA77_003980"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            509..1807
                     /gene="kgtP"
                     /locus_tag="POA77_003981"
     CDS             509..1807
                     /gene="kgtP"
                     /locus_tag="POA77_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDI6367340.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1804..2127)
                     /locus_tag="POA77_003982"
     CDS             complement(1804..2127)
                     /locus_tag="POA77_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDI6367341.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2173..3528)
                     /gene="pssA"
                     /locus_tag="POA77_003983"
     CDS             complement(2173..3528)
                     /gene="pssA"
                     /locus_tag="POA77_003983"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDI6367342.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3642..6302)
                     /gene="pat"
                     /locus_tag="POA77_003984"
     CDS             complement(3642..6302)
                     /gene="pat"
                     /locus_tag="POA77_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDI6367343.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6334..7032)
                     /gene="tapT"
                     /locus_tag="POA77_003985"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6334..7032)
                     /gene="tapT"
                     /locus_tag="POA77_003985"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDI6367344.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7101..7520)
                     /gene="trxC"
                     /locus_tag="POA77_003986"
     CDS             complement(7101..7520)
                     /gene="trxC"
                     /locus_tag="POA77_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDI6367345.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7727..8764
                     /locus_tag="POA77_003987"
     CDS             7727..8764
                     /locus_tag="POA77_003987"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI6367346.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8812..9501)
                     /gene="ung"
                     /locus_tag="POA77_003988"
     CDS             complement(8812..9501)
                     /gene="ung"
                     /locus_tag="POA77_003988"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI6367347.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9806..10189
                     /gene="grcA"
                     /locus_tag="POA77_003989"
     CDS             9806..10189
                     /gene="grcA"
                     /locus_tag="POA77_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDI6367348.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10245..10832)
                     /gene="eamB"
                     /locus_tag="POA77_003990"
     CDS             complement(10245..10832)
                     /gene="eamB"
                     /locus_tag="POA77_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDI6367349.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10935..11816
                     /locus_tag="POA77_003991"
     CDS             10935..11816
                     /locus_tag="POA77_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6367350.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYAIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2961 a         2984 c         3101 g         2893 t
ORIGIN      
        1 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
       61 gaatttgcct ggcggccgta gcgcggtggt cccacctgac cccatgccga actcagaagt
      121 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
      181 ggcatcaaat taagcgaaag gccatcagtc acgatggcct ttttgcgttg gcgcagaaaa
      241 aaatgcctga tgggacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
      301 tgcggtttcc ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta
      361 agctcctcaa taaccatttt cctgctaact aaattcatgg ttaaggttgc ataatgatac
      421 gcaacaaatg tataacattt tctttaccaa aagaaataaa taaaagcgac cgacaaaagc
      481 atcggattac ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa
      541 actgacaagt agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa
      601 tctggtcgag tggttcgatt tctatgtcta ctcgttctgt tcactctact ttgcccacat
      661 tttcttccct tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc
      721 gggattcctg atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg
      781 tcgcaaaaaa tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc
      841 ctgcctccca ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgcgcg
      901 tttatttcag ggattatctg ttggcggaga atatggcacc agcgccacct atatgagtga
      961 agttgccgtt gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg
     1021 cggacaactg ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc
     1081 actcagagag tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc
     1141 gttgtggtta cgtcgtcagt tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga
     1201 agctggatct ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt
     1261 taccgctgcg ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt
     1321 aaatactgcg ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt
     1381 attcatgctt attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc
     1441 aatgttatgt ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca
     1501 aaacgtttcc tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag
     1561 tttttataca tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc
     1621 attaggcgtt ggcctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta
     1681 cgtagcgttg tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt
     1741 gatggccgtg gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg
     1801 tctttagtga cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca
     1861 ggccaaatcc ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga
     1921 cgcccccaaa ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc
     1981 ctgatgctgt gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg
     2041 agctttatcc tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa
     2101 tagcgaacag acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg
     2161 acggggttgt gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg
     2221 gcggatgagt ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg
     2281 cttaacgatg gtggtatgct cgcggatcag ctccagttct ttctctcgct gtggcgccag
     2341 ctcaagttgc ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg
     2401 gttcaggtta ttaccggtga tcaacatcca cttatcatca acccacatcc cttttaggtg
     2461 ataggtgttg tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata
     2521 ctgcaaacgg ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat
     2581 tatcttgaaa ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat
     2641 aatttcgacc tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg
     2701 caggttgaag tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa
     2761 aatggtcttg ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg
     2821 atcgttgtcg gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag
     2881 acgaatatcg ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac
     2941 gccgcggcca ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg
     3001 gttacggata agatgataac ggtcgtagcg atatttatcg tgctgatgca ggtaaacatc
     3061 gttcaggctg gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag
     3121 aacaccaagg gcttcacggg tattgattgg aatgccataa accggaacat ctacgcccgg
     3181 attttcctgc gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat
     3241 gcgtccacgt tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg
     3301 ccttttagct tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata
     3361 cagggcgaca atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg
     3421 gaagtcggcg ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag
     3481 ttgggcaagg tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt
     3541 cttctctgtt cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa
     3601 caccagtccc gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgttgg
     3661 gcaagattta gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg
     3721 agcttgcggg ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt
     3781 tgtagtccgt gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct
     3841 aaccctttga gatccgagcg aaccagcacg gcaaattcgg catcgatgtt atcagggtcg
     3901 gagatcgcac gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca
     3961 aacgccattt cccgatcgta gtcgatctgc gtcatgttgg ctaaatcttc atgagtaaat
     4021 tcgttgatct cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat
     4081 tgctgaagtt gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt
     4141 ttcaattcta cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg
     4201 ttatcgcctt caaacggcgc gatatccagc gtgacatcca gcgcggtaaa ttcactgcca
     4261 gaagccagca aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaggttg
     4321 gaaacctgca ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc
     4381 gcacgaatct ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc
     4441 agcggcggca gtgcgacgac ggcttgatct tcaggacgcc actccacacc tccctcaccc
     4501 agcatgatca acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg
     4561 ccagcccggt tagccatact ttgcaccaac aggccgtgga tccgcgcctg cggccatgcc
     4621 atttttacgc gatcgaaaat agcgtttgcc gcttgctgga cttcattggc tgtacgcagg
     4681 tacagcatta cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc
     4741 gccaccggat aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc
     4801 caggtgggga gcgtgttcat gccatacgct tgcaggatag gctgaacttc atgggtatcg
     4861 agcgacgtag ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg
     4921 gaagtcagat tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac
     4981 tccaccatat gcataaacgc ggtgatggtg ccttccggcg tgcgataggt cggcagcccg
     5041 gcttcgctga ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc
     5101 aaagagacat atttgctgcg gggatgatgc tttaccgctt caattaattc ttgcgcgctt
     5161 tctgttgcgg gagccgctgc gctgggtgaa tgaataacca tcagcgcatc aaaatcctgg
     5221 ctgtggagca gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga
     5281 tcgagcgggt tagagattgc cacatgtcca ggcagtgcat cgcgcagttt ctggcaggtt
     5341 tcttcgctta gcgttgccag cttgccattg tgtgaccata aggcatccag cgccagcgcg
     5401 gcaggcgcag caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg
     5461 ctaagggttt ccaccgccga aaacagctcg tgggtgtcct gtacccgcaa caaaccggca
     5521 cgctgaatag ccgcatccca cgccggatcc attcctgccg tcgtgttgag cagtcgctgt
     5581 gccgccgggc tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg
     5641 gccgccgaaa caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg
     5701 gttttactgt cgcgcgcaag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg
     5761 ccgagcgcaa taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg
     5821 ttggagacgg cagccgattg tgaaataaac gccagcttgc cgcgtttaat cggcacaggc
     5881 gaaaagctgg cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtcctagc
     5941 aggcgcatgt tatggcgcag ggcgcaggcg cggagatctt catgttgcga tgccggggcg
     6001 gaaagaataa tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta
     6061 cggctggcat tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc
     6121 ggccaggcta acacacccaa caccgctttc caggctggcg tcaccggaag taccggtccg
     6181 ttaaagcctc ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc
     6241 gatgcgccaa ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc
     6301 ataccggtct cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt
     6361 gatgctaccc agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaaag
     6421 ctcgcctaac cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac
     6481 ctcggcagta caatattggc cttcagcctg ggcttcacgc aggcgatagg cagaaagccg
     6541 ggaaagatcg acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt
     6601 gcgcgcttcc ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg
     6661 tgtgaagatc acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggttg
     6721 atagtccggg ttttgtacca gatccagcaa gtcttgcgag gggtcggtac gcgaccattg
     6781 aaacgcaacg gtatcaggca aaatatcagc aatcagacgc ccggtgttac ttggcttcat
     6841 tggctcggtg tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt
     6901 cgaacagaga cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc
     6961 gcgggcaaga aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc
     7021 gttttcggtc ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca
     7081 caagatgtgc cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc
     7141 ggtactgcgc cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt
     7201 atactacgaa ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca
     7261 aagcgcactt taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg
     7321 caggggccgc accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat
     7381 ttgtcgagtg tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag
     7441 cgtccgcatt ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa
     7501 tgggtacaaa cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga
     7561 cgccggtatg tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg
     7621 ttttattcaa taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg
     7681 acttcgggta atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat
     7741 gaaaggtaaa agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa
     7801 acgtacccac aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga
     7861 cggtggccgt cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga
     7921 agattcgccg tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga
     7981 tcacggtggc atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc
     8041 ggaagaaacc cgcgtctacg gcgaaaatgc atgtcaggca cttttccaga gccgtccgga
     8101 agcgattgtt cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg
     8161 ctggatggca gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc
     8221 gtcaggcacg gaacatcacg gcggcgtttg cttcttgatc aaaaagcgta acggtacaac
     8281 cgtgcagcag tgggtaagcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga
     8341 atctaacccg cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg
     8401 tgtcgtggtg caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg
     8461 cggcgcagag cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt
     8521 ccgtcaggca ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa
     8581 aactagtctg ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga
     8641 tgccgcacgc gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg
     8701 gctgaatatc tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc
     8761 ctgataaatc agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact
     8821 ctctgccggt aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg
     8881 atttgccagc acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg
     8941 atgcggtgct ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc
     9001 atgtgatccc cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac
     9061 tttatcagta aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac
     9121 cgtcaacaca gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg
     9181 attagggcgg gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga
     9241 cggaggaatg gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc
     9301 gtgataagga tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa
     9361 cgcgttaaag acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc
     9421 gacggtctga agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg
     9481 ccaggttaat tcgttagcca tctcgctctc ctgcgaatct tcaatccgcc tagcttaact
     9541 gcttctttct acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat
     9601 gtaaattggt aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg
     9661 accacaatcg atgtgtggtt tttattgatt taaatcaaag aatcaagggt ggttgaggag
     9721 tatatataca ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca
     9781 aataattttg ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa
     9841 cgacgatctg ctgaactctt tctggctgct ggacagcgaa aaaggggaag cacgttgcat
     9901 cgttgcaaaa gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat
     9961 tgaataccgt gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca
    10021 cctgaacgtt aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa
    10081 atatccgcag ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc
    10141 ggaacagcag cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag
    10201 caagttacta agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt
    10261 accgcgcaat agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga
    10321 aacaatcgct gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc
    10381 atcgccagca aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca
    10441 aacgtcgaca gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca
    10501 aagctggccc aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg
    10561 gcgattttcc acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc
    10621 gctgccgggt caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc
    10681 aaaaatccca gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta
    10741 gcagagctaa gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa
    10801 gtccaaaaag cacttaaaag ggtcggtgtc acttgttatc tcctctgttt tggtagacag
    10861 tacccgacac ggaacattta aaaaaatgaa tgtttttata ttctagattc gatattttcg
    10921 ataggtttgg ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg
    10981 gttccttttt gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata
    11041 ttcagcagct tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt
    11101 gccttacccg cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg
    11161 atacgcttcg tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt
    11221 cgggcgaaac gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg
    11281 caccaaaagt gcgtttatcg ttgcagtcgc tgaactgtta cgcgatccgt gatgcgctac
    11341 tgaatgatga ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc
    11401 gacgagagtt gggtgaacaa tcgctggtgc tggtggcttc accgcaaatt gcagatgtcg
    11461 attttactga gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg
    11521 tcttccggca gatatttgag agcacgctgc gtcagcggcg gatcacggtg gaaaacacca
    11581 ttgagctgtt aagcattgaa agtatcaaac gttgtgtcgc ggcgaatatc ggtgtcagtt
    11641 acttgccccg ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt
    11701 ttggcgaaca gtcgcagacc attacggcca tgtgcgcaca ccatgctgga aaagcggtta
    11761 gcccggcgat gcacactttt attcagtgtg ttgaagagag ttttgtggct ggataaaaat
    11821 aatgtcggga ttaaacccgg catttaactt gtcgacaaag tgcgctttat tcatgccgga
    11881 tgcggcgtga acgccttatc cggcctacaa gatcgtgcaa attcaatata ttgcatgag
//
LOCUS       DAMZII010000148        11739 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZII010000148 DAMZII010000000
VERSION     DAMZII010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.148"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(57..458)
                     /gene="crl"
                     /locus_tag="POA77_003992"
     CDS             complement(57..458)
                     /gene="crl"
                     /locus_tag="POA77_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDI6367351.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(516..1760)
                     /gene="frsA"
                     /locus_tag="POA77_003993"
     CDS             complement(516..1760)
                     /gene="frsA"
                     /locus_tag="POA77_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDI6367352.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(1852..2310)
                     /gene="gpt"
                     /locus_tag="POA77_003994"
     CDS             complement(1852..2310)
                     /gene="gpt"
                     /locus_tag="POA77_003994"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6367353.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2571..4028
                     /gene="pepD"
                     /locus_tag="POA77_003995"
     CDS             2571..4028
                     /gene="pepD"
                     /locus_tag="POA77_003995"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDI6367354.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4085..4699)
                     /locus_tag="POA77_003996"
     CDS             complement(4085..4699)
                     /locus_tag="POA77_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDI6367355.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4696..5835)
                     /locus_tag="POA77_003997"
     CDS             complement(4696..5835)
                     /locus_tag="POA77_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDI6367356.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSISGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(6081..6533)
                     /locus_tag="POA77_003998"
     CDS             complement(6081..6533)
                     /locus_tag="POA77_003998"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6367357.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(6530..7585)
                     /gene="dinB"
                     /locus_tag="POA77_003999"
     CDS             complement(6530..7585)
                     /gene="dinB"
                     /locus_tag="POA77_003999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI6367358.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7656..8441)
                     /gene="lafU"
                     /locus_tag="POA77_004000"
     CDS             complement(7656..8441)
                     /gene="lafU"
                     /locus_tag="POA77_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDI6367359.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            8413..10125
                     /locus_tag="POA77_004001"
                     /pseudo
     CDS             8413..10125
                     /locus_tag="POA77_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10230..10508
                     /locus_tag="POA77_004002"
     CDS             10230..10508
                     /locus_tag="POA77_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDI6367360.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            10501..10857
                     /locus_tag="POA77_004003"
     CDS             10501..10857
                     /locus_tag="POA77_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDI6367361.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(10914..11687)
                     /locus_tag="POA77_004004"
     CDS             complement(10914..11687)
                     /locus_tag="POA77_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI6367362.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         2864 a         3085 c         2962 g         2828 t
ORIGIN      
        1 atgacctatc agttttaata gatattgccg gatgtgatgc atccggcaca tttcactcac
       61 gccgttaact tcaccggctc gtcacgaaaa tcatccgccg gttccagctt cagattcagc
      121 gtcgtcagca gttcacgcag cttctcgtga aactcacgca gggtgtgctc cagtcgttca
      181 accacttcag tatcttttac cggaacactc ttccagtcgc ctgctttatc gaacagacca
      241 aactggtagc tgtaggtaaa acgggattcc tgcgcttcca gctccatcca ccagccccag
      301 aactcacgca cttccggtgc cggtttcacg ttgacgcata cagccagaca atcgaaaaag
      361 aatcgattat cttcgcactt accttcacga atatacgggc ctagtgcggt aaattttttg
      421 atcaatctgc tcttcgggtg tccactcggt aacgtcattg caatctcctg ttgtgatgca
      481 actgttttac caaattggta aaatttagca aatttttaac acaaacgttt ttcgatccag
      541 tcggtgattt cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa tgggatctct
      601 aataatttac cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc cgggctgaac
      661 ggatcgttct tccagtagcc tgataacatt ggcgttgggc agcgacgtcc cagcaatcct
      721 tgcactttta atgaatagcg attcaactcc acgcgcaacg cttcgtcgga agcatcatgc
      781 atccccaaac gactcgccag aacgtcaaga tacatttccg gcacctgttg ctggcactta
      841 aaatcactca acagggtatg aactaccgga ccaagacagg caaccgcttt cagacgcggc
      901 gattcaaggt atgccagacg cacggcaacg ttagcgccga aacggaaacc aaaggccgcg
      961 acgcgagtgt gatccaccca cggtacgtta ggcagcgcct ttaagacgtg ctgatgcaac
     1021 aggctggagt cctgggtgag cttccatttt gaagaaaagc ccaccgacgg catatcaata
     1081 gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat acaggctgta atagtccgtc
     1141 tgcatcgcat ccagaccacc acacattaat actgtcggga acgggccatc gcctttcggc
     1201 atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg taaactccat ctgccgcatc
     1261 gtgcccggca gacgctgggc ggcctcttca taggcgcggt ttgataaagc ctgcgcttgc
     1321 tcggccaggt catctccttt cagatgagga taggcggcaa tgttgtacaa cgtagccgca
     1381 tgcagccagt gtcgaccact gagttgcgga tcttcttccg cacaggcttt ttgttgccac
     1441 accatcgcct gggtggccca ctcataaatc cagttgccac cacggtagcc aatcacggta
     1501 tcgtataaat catcatcggt acgttcagca tcgctcatca caatacgtgc ctggacgtcg
     1561 aggatttcac gtgggtcaat gccgcgccag atccacatca gacggttaat catgcgatac
     1621 cagtgaggga tggttttacc atcaagagcc gactgcacag gcggttgtgc gccgtgatta
     1681 aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg
     1741 ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa ctattgtaac
     1801 ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag attagcgacc
     1861 ggagattggc gggacgaata cgacgcccat atcccacggc tgttcgatcc aggtatcttg
     1921 cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg cgaagatagt
     1981 cacaaaatgt gcttttggat acatttcacg aatcgcaacc gcagtaccac cggtatccac
     2041 taggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca gcactttaag
     2101 ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat gacgaatacc
     2161 cagttcacgc gccagtaacg cacccggtac cagaccgcca cggcttacgg caataatgcc
     2221 tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga tctgcaacat
     2281 gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt tatctacggc
     2341 ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg gcgcactaaa
     2401 aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg acgcgggata
     2461 aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa
     2521 ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac gtgtctgaac
     2581 tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt tctattcctc
     2641 acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca aaagagaaag
     2701 gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct gctaccgcag
     2761 gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg ccgcagaaaa
     2821 ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt gatggcgaat
     2881 gggttaaagc gcgcggcacc acgctgggtg cagataacgg cattggtatg gcgtctgcgc
     2941 tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg ctgaccatga
     3001 ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg caggctgata
     3061 ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt gcggggggta
     3121 tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt tttgaaacct
     3181 tcaagttaac cttaaaaggt ctaaaaggcg gtcactccgg cggggaaatc cacgttggcc
     3241 tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa gaactggatc
     3301 tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt gaagcctttg
     3361 cgaccattgc tgtcgcagct gataaagtcg acgtcctgaa atctctggtg aatacctatc
     3421 aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg ttgctggact
     3481 ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt attcgtctgc
     3541 tgaacgccac cccgaacggt gtgatccgca actcagacgt ggcaaaaggt gtggtcgaaa
     3601 cctccctgaa cgtcggtgtg gtgaccatga ctgataataa cgtagaaatt cactgtctga
     3661 tccgttcact gatcgacagc ggtaaagact acgtcgtgag catgctggat tcgctgggta
     3721 aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag ccggacgcta
     3781 attctccggt gatgcatctg gtacgtgaaa cctatcagcg tctgttcaac aaaacgccga
     3841 acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa
     3901 tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat gagcaagttc
     3961 acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa gaaattccgg
     4021 cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc agtttcatcc
     4081 ttcattatcc ttcgataaaa gccatccctg taaatgtccg tctcgggttg ccacgttcaa
     4141 tctggtgatg gaacattcgc cgctgcgatt tcagcaccgc gctattttcc tgttgctgct
     4201 gctccagctt ccaggcaatc agcaatcgtg ccagccgctt gttagcatgc tgactacgct
     4261 ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc gccgagtcgg
     4321 ttttattgac atgttgaccg cccggccccg acgaacgcaa cgtctcataa cggattgcat
     4381 ccgattgttc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag tttttgcgcc
     4441 catgatgagg ccgatacgga ctcggacaaa tccactgaat agtgccacac cagctttcgc
     4501 ttaatgccca tgcgttatcg ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt
     4561 agcggcccgt ttctgtttcc aacaccgtta ccgcgacgtc ttgtcgggca gcttctttaa
     4621 ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc tgcgctgagg
     4681 agagttgtag caagatcatc cttttttccc tccactgttt ttgagcgtta gcaccggacg
     4741 cagccgcgcg acaggaataa ttaaaccggc ctgcaccaga cattgcacca cgctttcagc
     4801 cgatttatag gcttgtggcg cttcttcaaa gatgagttgt ttatcgcgac aaattacccg
     4861 actgccaagt tcagtacgcg agagttgcgt cgccgtgtat ttcgctgcca gacgcccttt
     4921 acactcggtg cgcccccatt tacgccctgc cccatgcgcc agcgagtgga gcgttttttc
     4981 gttcgcaaca ggctgtacaa tccaggagta atcaccacgt gaaccgggaa taatcaccag
     5041 accgttgtca tccggcgtgg cacctttacg atgcaaccag ccctgttgat cgccgatttg
     5101 acacgcgcta acaaagttat gcgccacatc cagaaccgga cttcccgtgg ccttaacttg
     5161 ttgcattatg cgcaaagcga tcatctggcg attaatacac gcaaacgcca gcgcatcgtc
     5221 atgttccgca atataccgta gcgcgtcgtc actgccttga ggcaaaccat gatgcgagaa
     5281 cgaggcaata tgccgctgta aaatagactg ccccaccccc cgcgagccgc tatgaaccaa
     5341 cagttgcaga tgttgtgcat ccagacctgc cagtgcaaac agttcagcgt tgatgatttg
     5401 atcaacttgt tgcagttcag cgaaatgatt gccgccactg atagagccaa gcgagctgcg
     5461 ccacggatgc tgtgcaaatg ctgacggcag gttttcctcc agccagcttt cttcagcaac
     5521 gtcatccaga tctgataatc gcttttcaaa cttatcggcg ttgtatttgc gagtgagaat
     5581 atctgtttgc catagcgcca taccgcagcc gatatcattg ccgaccagtg ccggataaaa
     5641 acgaccaaca gagaagaacg ctgcgccaat cggatagccg cgtccgggat gtaaatctgg
     5701 catccccact acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc
     5761 taaactctcg atccacaggt catcagatgc gatggtaaat accgcatcgg ataagggacg
     5821 aatataattg cccatatacc attccgtaaa tagactcgtc atacttcaag ttgcatgtgt
     5881 tgcgacttga attattgaga gtaacaatta aaaaatcagg aaatggcaga cgaatccagg
     5941 gcaagaaaaa aagaattaat cctggaaagg tctgcaaaga gtcattgata aaggatttgt
     6001 catgatttac ctccttgcag tttgtggaat aagaaaacgc gcacatacta ggcttgcgtt
     6061 attttcattg caagctggat ttaatgttgc tgtttataac gcatataaaa attaataaac
     6121 cactctcccc gacattcaac gcgttgctgc ttaactgtct gaaaaccata acgttcaaaa
     6181 aagggttttg cggttatgct tgcgtccacc gtaagttcgg attcagactt aatcaaaggt
     6241 tttaacaaag cgctggcaac cccacggcgg gtgtattcag ggtcaacaaa taacatatcg
     6301 atatagtgtt caacgcaggt aataaagccg accagttttg cattaatgac tgcaacccgc
     6361 acttgtgatt tcgcgagttt ctccttccag cgagattcgt caatctgcgc ccaggcggca
     6421 atttgttgtg gtgaataatg ctgactggca gtcatcgtaa ccgctctaat gaaaatagcg
     6481 catagttgct gaaaatcgcc aggctgatag tctcttattt gtatgttatt cataatccca
     6541 gcaccagttg tctttccatt tgcgggtcaa gcaacgtcac atgcagcccc accagtcgca
     6601 caccgcgccc gccgcggcgt tcatcccagg ttttacgcgc ggtggcgatt aagtcagctt
     6661 tattcagccg cggccagacg tgctcctgag tggtttgctg aaaatcgtcg aactttaatt
     6721 tcaccccctg gcgggcaatc agtaaatcag gttttacctt tgccagacgg cgttcaagtt
     6781 ccggatacag ccgctcgata atcgcttcac attcagacca gtggtggata tcttccgcca
     6841 tcgtgcgttc cacgccgacg gattttcgca accgttcgct gttaacgtcg cgctcgtcaa
     6901 tcccctgact acgctcccac aaaatgcggc caaatttgcc aaagcgttta agcagtatca
     6961 ccagatcaca cttttgtaca tcaccgcagg tccgcagccc catcgcttcc agttttgccg
     7021 ccgagacttt gccgacgccg gggatttttg ccagcggtaa ggtttgtaaa aatgccggaa
     7081 cttctgccgg cgtaatcaca aactgaccgt tgggtttatt catgtcggag gcgattttgg
     7141 cgagaaactt tactggtgcc acgcccgcag acgctgtcag ttgcagctcg ctgaaaatcg
     7201 tctggcggat ttcctgggcg atgagggtcg cagaaccgtg gcaatggacg ctatcggtga
     7261 cgtcgagata agcctcatcc agtgacaatg gttcaatacg cgaggtgtag cgcgagaaga
     7321 tttcacggat atgatttgag gcttctttgt aggcgtcaaa gcgccccgga agcaaggtga
     7381 gatgcgggca taatttgagc gccatccctg tcggcatagc gctacgtacg ccaaatttac
     7441 gcgcgggata attggcggtg ctgatcaccc cccgacgttc gcggctgccg ccaatagcaa
     7501 tagggatatc gcgcagggcg ggattgtcgc gcatctccac cgctgcgaaa aagcagtcca
     7561 tatccacatg aatgatttta cgcattactc acctctcaac actggtaaag tatacagtga
     7621 tttcagggtt tgagaaatgc gtaaagattc agcatttaac gcgttcgctg cgaaagcacc
     7681 tgctgcttat ccagcttttg caccagcggc tgcaccactt tatccccatg ctgaccaaag
     7741 tattgataca gcgtatcgga cgcacttttg gtcagcacca taatctcaat gcgccggttg
     7801 cccgcgcttt gcggattttt ggaatccagc agcatctggt ccgccattgc gcttacctgc
     7861 atcactttat cttccggcat tccggcctct tccagcaccc gacgagccga aagcgcgcgg
     7921 tcacccgaaa ggttccagtt gttgtagata ttgtttttgt aagccatcgc atcggtatgc
     7981 ccggtaataa taattttgtt atcgagcgag tcgaaaactg gcgcgagctc caccagcagc
     8041 gttttaaaga acggcataat ctgcgcgctg ccgcgttcga acatattgcg attctggtcg
     8101 tctttaatca gcacgcgtaa tccctgggga acaatctcca tctccagatt cgcttccata
     8161 tgcgcatcac gggcaatggt ggtgatactg gttgccagct cgccaagctc ggtggcggat
     8221 ttcttcttca gcagcgccgt tttctcattc acatcacgcg cttttttctc cgtttcttcc
     8281 ggcaccgcca cggtggcagg ttttggcgga tgagacgggc tgattttatt taacggcgac
     8341 agcccaccac cgttaaaaat cgactgcccg tgcagcgcgg cgataatgct ttcacgctcg
     8401 gatttactga cgctgttgac aatcaacttt attgtcgtca ccaaaggggc cgagcgtatt
     8461 tccgaggttt cagcccgctt taccctggat gcgatgcccg gtaagcagat ggcgattgac
     8521 gccgatctta atgccgggtt gatcaatcag gcgcaggcac aaacccgacg taaagatgtt
     8581 gccagcgagg ccgatttcta cggcgcgatg gacggggcat cgaaatttgt gcgcggggat
     8641 gctatcgccg ggatgatgat tctggcgatc aacctgatcg gcggcgtctg tatcgggatc
     8701 ttcaaataca acctgagcgc cgacgccgcc ttccagcagt atgtgctgat gaccatcggc
     8761 gacgggctgg tggcgcaaat cccttccctg ctactctcca ccgcggcggc gattatcgtc
     8821 acccgcgtca gcgacaacgg cgatatcgcc catgacgtgc gtcaccagtt gctggcaagc
     8881 ccatcggtgc tctacaccgc taccggaatt atgttcgtac tggcggtagt gcctggaatg
     8941 ccacatctgc cgtttttgct gttcagcgcc ctgcttggct ttaccggctg gcggatgagc
     9001 aaacgcccgc aggcggcgga agcggaagag aaaagtctcg aaacgctgac ccgcaccatc
     9061 accgaaacca gcgagcaaca ggtcagttgg gaaaccattc cgctgatcga gcccatcagc
     9121 ttaagcctcg gctacaagct ggtggcgctg gtggacaaag cccagggcaa cccgctcacc
     9181 cagcgtattc gcggcgtgcg acaggtgata tccgacggca acggcgtgct gctgccggag
     9241 atccgcattc gggaaaactt ccgccttaag cccagccagt acgctatttt catcaacggc
     9301 attaaggctg atgaagcgga tattccggcg gataaactga tggcgctgcc ctccagcgaa
     9361 acctacggcg agatagacgg cgtgctgggg aacgacccgg cgtacgggat gccggtgacc
     9421 tggatccagc ctgcgcagaa agcgaaggcg ctgaatatgg ggtatcaggt gatcgacagc
     9481 gccagcgtga ttgctaccca tgtaaataag attgtgcgca gctatattcc tgatttattt
     9541 aactatgacg atattacgca gttgcataac cgtttggcgt cgatggcacc gcgcctggcg
     9601 gaggatttaa gcgcggtgct caattacagc cagttgctga aagtgtaccg ggcgctgctg
     9661 accgaaggcg tttccctgcg cgatatcgtc accatcgcca ccgtgctggt cgccagtagc
     9721 gcggtgacca aagatcatat tctgctggca gccgatgtgc gcctggcgct gcggcgcagc
     9781 attacccatc cgttcgttcg caagcaggag ctgacggtgt ataccctgaa taatgagctg
     9841 gaaaatctgc tgactaacct agtgaatcag gcgcaacagg gcggtaaagt gatgctcgac
     9901 agcgtgccag tggacccgaa tatgctcaac cagttccaga gtacgatgcc acaggtgaaa
     9961 gagcagatga aagcggcggg gaaagacccg gtgctgctgg tgccaccgca gctgcgccct
    10021 ttgctggcgc gttatgcaag gttgtttgcg ccggggctgc atgtgctgtc gtataacgaa
    10081 gtgccggatg agctggagtt gaagattatg ggggcgttga gttgatgggg gattcttttc
    10141 catgctttgc agcacctgac accattaatt gacgtcacac tgcaaagaga ctacgatgac
    10201 accatacaat gacgtcatgg attgatagta tgattaaggt cagcgcgcta ggttctcgtc
    10261 atagaaaaac attcgacgat gtaatgacgg agccgccaaa atctggaatt aaatgggatg
    10321 atgttgttgc actcattaaa gctgtgggtg gaacaataaa aaataacaac ggctccagac
    10381 gaaaatttca gattggaacg accaaattca gcacacatga gccacatcca aagaatgtga
    10441 tggataaagg cgcagtagca ggtcttaagg agtggtttgt taactgtgta ggggtagatt
    10501 atgaataaag catcgaacat tatcacaatt aaaggtcaac cagccgcagt gacatttgaa
    10561 gccgatatca acgcatttcg cggcaagttc ctcaatgtaa acggttattg tgactttgta
    10621 gccacaagta ttgaggcatt atatcgtgaa ggcgaaagcg cgctggatga gtggatggcg
    10681 gattgtgaag aggacggtat tgcccctttt cgtgaagaag aagagcaaaa acgcctgacg
    10741 cttcgtgttc ctcatcacat tgattcgcgg ctgaccatcg tcgccagaca acattccatt
    10801 tcaaaaaacc agctcattgt tgatgtctta gaacgtgagt tttcagccca gatgtaaaca
    10861 ggtcggatgc cgcgtggcat ccgacctttt gccagttcgt caatattacc catttacaaa
    10921 atcgtctctt ccgtcaatat ccgccgcgcg cctaaaaagt gatcctgcca gaaaggttca
    10981 gctaaccgac ttacccgaat ggtttccccg gtgcgcggtg actcaataaa ctgaccgtcg
    11041 cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa aaatagcaaa
    11101 tctccccggc gcaggtcgtt attcgccaca atggttgccc ggcgatagtg atacatctca
    11161 ttagccgtgc gtgggagttt cgcctcaagg atcttgttgt aggcataaaa aactaacccg
    11221 ctacagtcga agccttgatc gggccgcgta ccgccccaga cgtaaggctt gccaagctgt
    11281 tgctcaagac ggtgaatcgc cacttcggtg atgttatgca gatggtcgct gcttaagaaa
    11341 tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg cgcaggaaac
    11401 cattctggat gctgttttat ctgttcgcgg ttgtgctggc gtagcgccct tctgctttcg
    11461 gcattgcctt ccacaatata gctggcctgt tttttcagat gggtttggta ctgttttaat
    11521 aaacgcgcac gattttgcat ccgctggcgg gcggcgtagc tgaaagtgat atgtgatgtg
    11581 gtggcgctgg caaatgaaga aaacggcaag caaagcaaca gtaatcccga caacacaaaa
    11641 gagggaatcg acggcaagga catactcaat aatggctgat catgcattga tgttgcagtg
    11701 tatcccggcc cgtacctgaa caaacttaat aaaccatta
//
LOCUS       DAMZII010000149        11608 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZII010000149 DAMZII010000000
VERSION     DAMZII010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.149"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..278
                     /locus_tag="POA77_004005"
     CDS             <1..278
                     /locus_tag="POA77_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDI6367363.1"
                     /translation="YWLIDAFFAFNKYDWMLEPGDNICSIPSVSGEDRILQAMIAAFF
                     LLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
     gene            complement(324..1673)
                     /gene="qseC"
                     /locus_tag="POA77_004006"
     CDS             complement(324..1673)
                     /gene="qseC"
                     /locus_tag="POA77_004006"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDI6367364.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(1670..2329)
                     /gene="qseB"
                     /locus_tag="POA77_004007"
     CDS             complement(1670..2329)
                     /gene="qseB"
                     /locus_tag="POA77_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDI6367365.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            2481..2873
                     /gene="ygiW"
                     /locus_tag="POA77_004008"
     CDS             2481..2873
                     /gene="ygiW"
                     /locus_tag="POA77_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDI6367366.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            2926..3408
                     /locus_tag="POA77_004009"
     CDS             2926..3408
                     /locus_tag="POA77_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6367367.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            3954..6212
                     /gene="parC"
                     /locus_tag="POA77_004010"
     CDS             3954..6212
                     /gene="parC"
                     /locus_tag="POA77_004010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI6367368.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            6445..7182
                     /gene="plsC"
                     /locus_tag="POA77_004011"
                     /gene_synonym="parF"
     CDS             6445..7182
                     /gene="plsC"
                     /locus_tag="POA77_004011"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDI6367369.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            7257..8669
                     /gene="ftsP"
                     /locus_tag="POA77_004012"
     CDS             7257..8669
                     /gene="ftsP"
                     /locus_tag="POA77_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDI6367370.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            8780..10999
                     /locus_tag="POA77_004013"
     CDS             8780..10999
                     /locus_tag="POA77_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDI6367371.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(11042..11299)
                     /gene="yqhH"
                     /locus_tag="POA77_004014"
     CDS             complement(11042..11299)
                     /gene="yqhH"
                     /locus_tag="POA77_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDI6367372.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11350..>11608)
                     /locus_tag="POA77_004015"
     CDS             complement(11350..>11608)
                     /locus_tag="POA77_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367373.1"
                     /translation="FIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNN
                     EEQELVLQRREGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         2705 a         2985 c         3108 g         2810 t
ORIGIN      
        1 cttattggtt aatagatgct ttttttgctt tcaataagta tgactggatg cttgagccgg
       61 gagacaatat ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga
      121 ttgcagcatt ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc
      181 gggaaatgtt cgagttttgg gtatatgtat tttcgctcgg tatttgtctg gtttgtggtt
      241 ggtggctttt ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt
      301 aacggctctc atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc
      361 gcattcccaa actcaacatt catgccatgc aatttggcga ttcgctggac aatcgatagc
      421 ccaagtccac tgccggtagc ggtttgtccg ggggggcgat agaagcgttc gccaattcgc
      481 gccagagcct ctggcgtcac accggggccg ttatccctca cgatgaaatt atcagcattc
      541 agcgtgacgt ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt
      601 cgcaccaaca aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt
      661 gtcagtcgca cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat
      721 tgcaggagat cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc
      781 cgcgatagag tgagcagttg atcaaccaga cgagtagcgc gatcgatccc gtaatgtaat
      841 tgaagcagcg cttttttccg cgcttgcgga tcatcgtcag aaagctgcgc aacttcggtt
      901 tgcactttca gcgccgttaa cgggctacgt agttcgtgag ctgcgtcgga ggtaaagcgt
      961 cgttcacgaa ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccaac
     1021 ggacgcactt cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc
     1081 atacgcagcg ccagcgccag tttgttcagc ggcgcgagtt cacgacccaa tagtaccatc
     1141 atgatgatta acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc
     1201 gccagcgcca tgtcttcacg gtattcccat tcctgaccaa caacgatgcg atatttgcca
     1261 tcaggtgagg tcatccagac aaaacgccat tgatctttat cgcccaccag ttgcccgtca
     1321 gcaaaacctt cccgttgata gctataagga atatcttctc cgttatcgcc atcgttaagg
     1381 accattctgc cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg
     1441 tgttttaatt tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg
     1501 agcgtactta accgcttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta
     1561 tccgttgttt gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt
     1621 aaaaagatta gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac
     1681 ctaatgtgta gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca
     1741 gatgatgcac atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt
     1801 tctcttcaat cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca
     1861 gggcaaattc ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc
     1921 cggggtcgag catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc
     1981 gcatcagagc ttccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat
     2041 cgtcagctcc cagacgcagc ccttctacac gttcctccag cgcatcgcgc gcggtcagga
     2101 tcagtaccgg ctcacgctga cctttttctc gccattcgcg caaaatatcg cgaccatcca
     2161 tgcctggtaa ggttaaatcc aggatcaccg catcataagg cgcgctataa agcgcctctt
     2221 ttccctgacg accttgtgta aaccagtcga cgctaaaacc cattttacta aggcccgttt
     2281 tgatgccgtc gccaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc
     2341 tgcgataacc gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcactaaa
     2401 ttttattttt ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga
     2461 cattaaaggg agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt
     2521 gcagcgcacc ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa
     2581 gtcaggccgg aggattccag gggccgaacg gcagcgtaac gaccgtagaa agcgcaaaat
     2641 ccctgcgtga cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg
     2701 atctctacgt gtttaaagat gccagcggta ctatcaatgt tgatatcgac cacaaacgct
     2761 ggaacggtgt gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag
     2821 actggaattc tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac
     2881 cgctcccggg acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca
     2941 ctggatgtaa acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt
     3001 agccctgagt tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg
     3061 ctttctcctg ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc
     3121 gcaccggaag cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat
     3181 cgttatggtg tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt
     3241 ggcgagctgg acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca
     3301 agcggcgaga aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg
     3361 gtgacagagc agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt
     3421 accggagtgt gagccaaatc actcgcgata agtttgagcg aacaatgcca caggcgtatt
     3481 gttgttgggc ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc
     3541 ggtgcgccgt aagccgtctc gggggtaccc atgagcaagc cgcacttatg gcaggaggcg
     3601 cggggcactg gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact
     3661 ttgatgcttt aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg
     3721 acccccatgc cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa
     3781 gtctacagca aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg
     3841 tccggcggcg ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct
     3901 ccggcaatat tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg
     3961 atatggcaga gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca
     4021 tgtacgtgat catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc
     4081 gccgcattgt gtatgcgatg tctgaactgg gcctgaatgc cagcgccaaa tttaaaaaat
     4141 cggcccgtac cgtcggtgac gtactgggta aataccatcc gcacggcgat agcgcctgtt
     4201 atgaagcgat ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc
     4261 aggggaactg gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat
     4321 cccggttgtc gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact
     4381 gggtgccaaa cttcgacggc actttgcagg agccgaaaat gctgcctgcc cgtctgccaa
     4441 acattttgct taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc
     4501 ataacctgcg tgaagtggct caggcggcaa tcgcattaat cgaccaaccg aaaaccacgc
     4561 tcgatcagct gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca
     4621 cttcgcgcgc cgagatccgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg
     4681 cggtgtggaa gaaagaagat ggcgcggtgg ttatcagcgc attgccgcat caggtttcag
     4741 gtgcgcgcgt actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg
     4801 acgatctgcg cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt
     4861 ccaaccgcgt ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa
     4921 agagctatcg tattaacctt aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc
     4981 tgctggaaat cctctccgaa tggctggtgt tccgccgcga taccgtgcgc cgccgactga
     5041 actatcgtct ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt
     5101 ttctcaacat cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc
     5161 tgatgtcgcg gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc
     5221 gtcatcttgc caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag
     5281 agcgcgacca gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga
     5341 aagaactgca ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac
     5401 gcgaagaagc gaaagcgatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg
     5461 tgctgtcgca gatgggttgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc
     5521 tgaattataa agcgggtgat agcttcaaag cggcggtgaa aggtaagagc aaccaaccgg
     5581 tagtgtttgt tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg
     5641 cgcgtggtca gggcgagccg ctcaccggca aattaacgtt gccgcctggg gcgaccgttg
     5701 accatatgct gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg
     5761 gtttcgtctg cacctttaac gatctggtgg cgcgtaaccg tgcaggtaag gctttgatca
     5821 ccttaccgga aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc
     5881 tgctggcaat cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc
     5941 tgtcgaaggg caaaggcaac aagattatca acattccatc ggcagaagcc gcgcgtggag
     6001 aagatggtct ggcgcaattg tacgttctgc cgccgcaaag cacgctgacc attcatgttg
     6061 ggaaacgcaa aattaaactg cgcccggaag agttacagaa agtcactggc gaacgtggac
     6121 gccgcggtac gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc
     6181 gccgtgccag cagcggtgat agcgaagagt aatatgccgg ggagggcctg tttcctcccg
     6241 cttgcaagga acgccggatg aaacgttaag tcgtcatccg gcagcgtatt caacccccgg
     6301 aaacgataaa aattcccttg aaaccgttgt ttattcatgc gttgcgatta acaatacgct
     6361 tttccagaga gcggctttta acaatgccct aacctgattt caggtgacgt acaatgccag
     6421 tctctcagac cttcagaggg tgttatgcta tatatctttc gtcttattat taccgtgatt
     6481 tacagcatct tagtctgtgt attcggctcc atttactgcc ttttcagccc gcgtaacccg
     6541 aaacatgtgg ccacctttgg gcatatgttt ggccgtcttg cgccgctgtt cggcctgaaa
     6601 gttgagtgcc gcaaacctgc agatgctgaa agctacggca atgctatcta tatcgctaac
     6661 caccagaaca actatgacat ggtgacagca tcgaacattg tgcaaccgcc gacggtgacg
     6721 gtaggtaaaa agagcttgct gtggatcccc ttcttcgggc agttgtactg gttaaccggc
     6781 aacttattga tcgacagaaa caatcgcact aaagctcacg gcaccattgc ggaagtcgtg
     6841 aatcatttca aaaaacgccg tatttccatc tggatgttcc cggaaggaac ccgcagccgt
     6901 ggtcgcggcc tgctaccgtt caagactgga gcatttcacg cggcaattgc ggcgggcgtc
     6961 ccgattattc ccgtgtgcgt ctctacaact tcgaataaga ttaatcttaa tcgactgcac
     7021 aacggtctgg tgattgtcga aatgctgccg ccaattgacg tcagtcagta tggcaaagat
     7081 caggttcgtg agctggctgc ccattgtcgt tcgataatgg aacaaaaaat cgccgagctc
     7141 gataaagaag tcgcagaacg cgaagccgcc ggaaaagttt aagtcggcaa tctgtatttt
     7201 tgcggggaac actttcctgc acggtattac tttagccagt tttacatgga gcaaatatgt
     7261 cactcagtcg gcgtcagttc attcaggcat cggggattgc actttgtgca ggcgctgttc
     7321 ccctgaaggc cagcgcagcc gggcaacagc aaccgctacc cgttccgccg ctacttgaat
     7381 ctcgccgtgg gcaaccgctg tttatgactg tacaacgtgc gcactggtca tttacgccag
     7441 ggacacgcgc gtcggtctgg ggaatcaatg gtcgttacct ggggccgact atccgcgtct
     7501 ggaagggcga cgatgttaag cttatttaca gcaaccgcct gacagaaaat gtctcaatga
     7561 cggtggccgg gctacaggta ccaggcccgc tgatgggcgg tccggcacgg atgatgtcgc
     7621 caaacgctga ctgggcaccc gtactgccca ttcgccagaa cgcagctact ctgtggtatc
     7681 acgccaatac tcccaaccgc acggctcagc aggtctataa cggccttgcc ggaatgtggc
     7741 tggtggaaga tgaagtcagc aagtcgctgc ctatccccaa ccattatggt gtggatgatt
     7801 ttccggtcat tatccaggat aaacggctgg ataactttgg tacgccagaa tacaacgaac
     7861 cgggaagcgg cggctttgtt ggtgatacgc tgctggttaa cggtgtacaa agcccgtacg
     7921 ttgaagtctc gcgtggctgg gtgcgcttgc gactgctgaa cgcgtcgaac tctcgtcgct
     7981 atcaactaca gatgagcgat ggtcgcccgt tacatgtgat ttctggcgat cagggattcc
     8041 tgcctgctcc tgtatcggtg aagcaacttt cgctggcacc gggcgagcgc cgcgagattc
     8101 tggtggatat gagcaacggc gatgaagtgt cgatcacctg tggcgaagcg gcgagcattg
     8161 ttgatcgtat tcgcggcttc tttgagccat ccagtattct ggtttctacc ctggtgctaa
     8221 cgctgcgccc aaccggcctt ctgccgctgg tcacagacag tcttccgatg cgcttgctgc
     8281 caactgaaat catggctggt tcgccaattc gcagtcgcga tatcagtctg ggtgatgacc
     8341 cgggtattaa tggacagctg tgggacgtca accgtattga tgtcaccgcg cagcaaggaa
     8401 cgtgggaacg ctggacggta cgcgcggacg agccgcaagc gttccatatt gaaggcgtaa
     8461 tgttccagat ccgtaacgtg aatggcgcga tgccgttccc ggaagacaga ggctggaaag
     8521 ataccgtttg ggttgacgga caagtggagc tgcttgttta tttcggtcag ccttcctggg
     8581 cgcacttccc gttctacttc aacagtcaga cgctggaaat ggcggaccgt ggctcgatag
     8641 ggcaactttt ggtcaatccg gtaccgtaat cttcatactc gcccgaggag ggcgagtatg
     8701 gaataccttt cgatttctct ttcgttccgg cttataatcc ccggcctttg attatttttt
     8761 tatcatttct ggaagcaaaa tgagctctat ctccctgatc caaccggatc gcgacctgtt
     8821 ctcctggccg cagtactggg ccgcctgttt tggaccggca ccgtttttgc cgatgtctcg
     8881 tgaagagatg gatcaacttg gctgggatag ctgcgacatc attttggtta ctggcgacgc
     8941 gtatgtcgat cacccaagct tcgggatggc gatttgcggt cgtatgctgg aagcacaggg
     9001 ctttcgcgtc gggatcatcg cccagccaga ctggagcagc aaagacgatt tcatgcgtct
     9061 gggtaaaccg aatctgtttt tcggtgttac tgctggcaac atggattcga tgatcaaccg
     9121 ttataccgcc gatcgccgtt tacgtcatga cgatgcctac acgccggata acgtcgcggg
     9181 taagcgcccg gatcgcgcca cactggttta tacccagcgt tgtaaagagg cgtggaaaga
     9241 tgtaccggtg atcctcggcg gtattgaggc tagtctgcgc cgtaccgcac attatgatta
     9301 ctggtccgat accgtgcgcc gttccgtgct ggtggattcg aaagccgaca tgctgatgtt
     9361 tggtaacggt gagcgtccgc tggtggaggt ggcgcaccgt ctggcgatgg gcgagccgat
     9421 tagtgaaatc cgcgatgtgc gtaataccgc gattatcgtg aaagaggcgc tgcctggctg
     9481 gagcggcgtg gattccaccc gtcttgatac ccctggaaaa atcgacccaa tcccgcatcc
     9541 gtatggtgaa gatttgccgt gcgcggataa caaaccggtg gcaccgaaaa agcaggaagc
     9601 caaagccgta accgtgcagc caccgcgccc gaaaccgtgg gaaaaaacct acgtgttgct
     9661 gccttctttc gagaaagtga agggcgataa agtgctgtac gcccatgctt cgcgcattct
     9721 gcaccacgaa accaacccag gctgcgcccg cgcattgatg caaaaacacg gtgaccgcta
     9781 tgtgtggatc aacccgcctg ctatcccgct ttctaccgaa gagatggaca gcgtttttgc
     9841 gctgccgtac aagcgcgtgc cacatccggc ctatggcaat gcccgtattc cggcttacga
     9901 aatgatccgt ttttcggtca acattatgcg cggctgcttt ggcggctgtt ctttctgttc
     9961 tattaccgag cacgaagggc gcattattca gagccgttcg gaagattcga tcattaatga
    10021 gatcgaagcg atccgcgaca ccgttccagg ttttacgggc gtgatttccg atctcggtgg
    10081 gccaactgcc aacatgtata tgttgcgctg caaatcgcca cgcgctgaac aaacttgtcg
    10141 ccgtttgtcg tgcgtttatc cggatatttg tccgcacatg gacactaacc acgaaccgac
    10201 gatcaacctc tatcgccgtg cgcgtgatct gaaaggcatt aaaaaaatcc tcatcgcctc
    10261 aggcgtacgt tatgacatcg ccgtggaaga tccgcgctat atcaaagagt tggcgaccca
    10321 tcacgtcggc ggttatctga agattgcccc ggaacatacc gaagaagggc cgttatcgaa
    10381 gatgatgaag ccgggcatgg gcagctatga ccgctttaaa gagctgttcg atacttactc
    10441 aaaacaggca ggtaaagagc agtatctgat cccctatttc atctccgcgc accccggtac
    10501 gcgtgatgaa gatatggtga atctggcgct gtggctgaaa aagcatcgtt tccgtctcga
    10561 ccaggtacag aacttctacc catcgccgct ggcgaactca accaccatgt attacaccgg
    10621 gaaaaacccg ctggcgaaga ttggttataa gagtgaagac gtcttcgtac cgaagggcga
    10681 caagcagcgt cgtttgcata aagcgttgtt gcgttaccac gatccggcaa actggccgtt
    10741 aatccgccag gcgctggaag cgatgggcaa aaagcatctg attggcagcc gtcgcgattg
    10801 cttagtgcct gcgccaacca ttgaagagat gcgtgaagct cgtcgccaga accgcaatac
    10861 ccgtccggcg ttgacgaaac atacgccgat ggcgacccag cgtcagacgc ctgctacggc
    10921 aaaaaaagcg tcgtctacgc aatctcgtcc ggtgaatgct ggtgcgaaga aacgccctaa
    10981 agcggcggtt ggacgttaaa aaaaatcccc ggcaactgac atgctgccgg ggattttttt
    11041 atcattctgc gtacatacgc aagcagcgca ggcaatcaaa atagtcctga gcatcaagac
    11101 gcgtattggc tctgttagct tcggatttgg cagcatagat ttgtgggcgt agtgctttca
    11161 tatcctgctc aagccgggcg attttggcat ttaatgtttg cacattgctc gccagctgat
    11221 tgaccttttg ctcattgacg cagccactga gcaagcaggt tgccagaaca acagctccca
    11281 cggtgaaaat cgttttcata tagtgttccc ttttatttat tgttccggct aattgttatt
    11341 taattcagct cattgcttta atctggcggc ggtttttgcc tcaatctgct taagtaggct
    11401 gccgctgttc gggcggataa aatcagcgat ttcccacttg ccttcgcgcc gctgtaaaac
    11461 cagttcttgc tcttcgttgt tgccattggt cagcgtaaaa cgaacatgaa tgcgggcgga
    11521 atcggcggtt ttctccagga ctcgaatttc atcaacgcgt gaaaatagcg actcttcgcc
    11581 aaaacagatc ggattgtcgt aaataaat
//
LOCUS       DAMZII010000150        11398 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZII010000150 DAMZII010000000
VERSION     DAMZII010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.150"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..894
                     /gene="eno"
                     /locus_tag="POA77_004016"
     CDS             <1..894
                     /gene="eno"
                     /locus_tag="POA77_004016"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6367374.1"
                     /translation="PLYRYLGGIGANLLPVPCMNIINGGVHARGQGADFQEYMIAPCG
                     APTFREAVRWGSEVYHSLRQVLLEKGMLTGVGDEGGFAPVVSSNREPLELIVLAIEKA
                     GYRPGEDIVICMDPASSEFYRDGKYHLRTENTELTSQEMTNYYQALVKNFPIALIEDG
                     LAEDDWAGWKILHGALGEQIELVGDDLFVTNVKYIQRGIDENLANSALIKLNQIGSLS
                     ETFDAVQLCHDNNWGTFISHRSGETVDSFIADMTVAMRAGHLKMGAPCRGERIEKYNQ
                     LMRIEDELGSSAQFAGKSAFK"
     gene            1235..1615
                     /gene="crcB"
                     /locus_tag="POA77_004017"
     CDS             1235..1615
                     /gene="crcB"
                     /locus_tag="POA77_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6367375.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(1995..3188)
                     /locus_tag="POA77_004018"
     CDS             complement(1995..3188)
                     /locus_tag="POA77_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6367376.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNALLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLAEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            3324..5048
                     /gene="iucA"
                     /locus_tag="POA77_004019"
     CDS             3324..5048
                     /gene="iucA"
                     /locus_tag="POA77_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDI6367377.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            5049..5996
                     /gene="iucB"
                     /locus_tag="POA77_004020"
     CDS             5049..5996
                     /gene="iucB"
                     /locus_tag="POA77_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDI6367378.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5996..7738
                     /gene="iucC"
                     /locus_tag="POA77_004021"
     CDS             5996..7738
                     /gene="iucC"
                     /locus_tag="POA77_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDI6367379.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            7735..9012
                     /gene="iucD"
                     /locus_tag="POA77_004022"
     CDS             7735..9012
                     /gene="iucD"
                     /locus_tag="POA77_004022"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDI6367380.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            9094..11295
                     /gene="iutA"
                     /locus_tag="POA77_004023"
     CDS             9094..11295
                     /gene="iutA"
                     /locus_tag="POA77_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDI6367381.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2718 a         2938 c         2974 g         2768 t
ORIGIN      
        1 cctttgtacc gatatcttgg cggcattgga gctaaccttc tgccagtacc ctgcatgaat
       61 attatcaatg gcggagttca tgcccgtggg cagggagctg attttcagga atacatgatt
      121 gccccttgtg gagcaccgac attcagggaa gccgtgcgct ggggtagtga agtatatcac
      181 tcgctccgtc aggttcttct ggaaaagggg atgctgacgg gggttggaga tgaaggcggt
      241 tttgcacctg tcgtttcttc taatcgtgag ccgctggaac tgatcgttct ggcgattgag
      301 aaagcagggt accgtcctgg tgaagacatt gtcatttgta tggacccggc atccagcgag
      361 ttctatcgtg atgggaaata tcatctgcgc acagagaata ccgagttgac ctcgcaggaa
      421 atgacgaatt actaccaggc gctggttaaa aatttcccga ttgccttgat tgaagatggc
      481 ctcgctgaag atgactgggc tggatggaaa atcctgcatg gtgcattggg agagcagata
      541 gaattggttg gcgacgatct gtttgtgacc aacgtaaagt atattcagcg cggcattgac
      601 gaaaaccttg ccaattcagc attgattaaa ctcaaccaga ttggttccct gagtgaaacc
      661 tttgatgcgg tacaactttg ccatgacaac aactggggca cctttatctc acaccgcagt
      721 ggtgagacag tggacagctt tatcgccgat atgacagttg caatgcgcgc aggtcatctc
      781 aaaatgggag ctccatgccg gggtgagcgt attgaaaaat ataaccagct tatgagaatt
      841 gaggatgaac tgggttcatc ggcgcaattt gccggaaagt ctgcctttaa atgacattat
      901 tgtgccaatc ccccaggctt acacgtcggg gttattctgg aggaatgatg aaagctcacg
      961 tttttgttat ttctatgctt actggcattg tcgtcaccta tgccgtgtta cttcttggtt
     1021 gcttgttcat cgataaaacg ctccccactg tagatgttgt cattctgtca cttgtcgtgg
     1081 gggcttctgc tcagcaattg tcgagggtat taatgtcaat aaatagaacc ttcccttatc
     1141 ttgcatgtag tcaaaggata taaattaaca atatcagtca aatgagaaca tattctgata
     1201 ttgttacgat aaaaaattat tttatgaggg gtacatgatt aaatcacttt ttgccgtcat
     1261 tatcggcggt tctgttggat gtactctgcg ctggttactg tccacacgct ttaacagtct
     1321 ttttccaaat ctacctccag gtacactggt ggtgaacctc cttgccgggt taattattgg
     1381 tactgcgttg gcgtactttt tacgacaacc acaccttgat cctttttgga aattaatgat
     1441 aacaacggga ctttgtggag gtctgagtac tttctctaca ttttctgttg aagtattcgc
     1501 attactccag gcgggaaatt atatatgggc tttgacgtct gtacttgttc acgtcattgg
     1561 ctccctcatc atgacagctt taggcttttt tatcataacc atacttttcg catgagaatt
     1621 cattaacgtt acaggtttat caactaatgt gacgcttagg atagaatttc gctccatggt
     1681 atgcgtttgc agtcattaca gtctccatga cgactgtaat gactgcaaac gcattacctg
     1741 acatatcagt ataaacaatg aattaacctc ttggcgtatt ttttacatca ctggccctca
     1801 ccccccaaaa ttctctctga aaaattgttg cggttaacgt tgccaatgta tccacccatt
     1861 tcagaagacg atctggcatc ctggtggcat gctgcgatgt gcttcaggtg agtgcactgg
     1921 gcggtcagca caggcggttt cgacagcgaa attgcacagg acgttatgcc ggctgtgagt
     1981 acaacatcat agccttactt gattgtacgg taatgccaaa tacatgcaga cactatggta
     2041 aataatacag cggcaaataa tgccgtcaca atcagcgcga ttgagacacc tgttttcgac
     2101 gccagtggca ccaacaatgc gcacagccca tagccaagtg tgtgactggc cgcaagccag
     2161 cccgcgcctg cgccgtcggc cagcttatca ttcagtaaaa gttgataagc gggtgttgcc
     2221 agagcggccc caaatgacag cactgcacag cccaggtaaa acaaccatag ctgttcagcg
     2281 agcatgatag ccagaccact actcatcaac accccggcac tcaggagcag agccactgga
     2341 gtcaggcgct gtggacggag taccacgaac tgcgcgataa gcgcagctat tgcggacagc
     2401 cccaacaacc acgccacctg ttggctaata gtggtggtat cagtatcgaa ctggcgagta
     2461 agggctggcg aaagtccaag ctgcatcatg ctcattgccg cagccagtaa cattgcgcaa
     2521 agcagataag gcaggcaatc ccgcttcagg ctgacgctct tgcgctccgg tgtgggctgt
     2581 ggtggtgtgc cgggcaaccg cagaagcatc accacagccc acaccggagc tgccatcagc
     2641 atccacactg gcgccagagg gtgaatgacc aacattgccg ccgcgcacag cggcccgaat
     2701 agcctgccgc agctcaggcc ggagctgatg gttgccagag cggccatgcg attcccttct
     2761 cccgctctct gcaatgccca gacctgacag gctggcacca ttgctgacac ggtcagaccg
     2821 tagacgatcc gggcgatgat taatattccc aaccccaccg ctgttgttac ggcaccggta
     2881 gccatcagca cgcttcccag cccaagcagg gtaaagctta cgccatatcc tcccagagcc
     2941 cacagcacca cagtcttact gccagtaagg gaaatttgct ttccccacca tggggaagat
     3001 ggcagaaaca gcattgagcc cagcatcagc agggcagccc atacagacag actcagattt
     3061 gtctgtatga ccagaacagg gagtacaacc agcagagcgt tctgtccgat accgagaaga
     3121 ccggcactga acgccagtgg ccagcaggac agggtttttt ggggcgtatc ttcgatctta
     3181 gatgacattg taatgtttca acaccatgta ttaattgtgt ttatttgtaa aattaatttt
     3241 tctgacaata acatttctca ttgataatga gaatcattat tgacataatt gttattattt
     3301 tactgtgtag gagctgtttg attatgatcc tgccctctga aaaatccgcc acagatgtgg
     3361 ctgcgcagtg cttcctgaat gccttgattc gtgaaaccaa agactggcaa ctggcggaat
     3421 acccgccaga cgaattgatt atcccgctgg acgaacagaa gtcgctccat ttcagagtgg
     3481 cttatttctc cccaacccag catcaccgct ttgcatttcc tgctcatctg gtcacggcat
     3541 caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat aagctccggc
     3601 atcaactttt tctgccagtt cccctctgcg aaactttcca ccaacgcgtg ctggaaagct
     3661 acgcccatac gcaacagaca attgatgccc gccatgactg ggccatcctg cgtgaaaaag
     3721 cgttgaattt tggcgaggct gagcaggcac tgctgacagg acacgctttc caccctgcac
     3781 ctaagtctca tgaaccgttt aaccgccagg aagctgaacg atacctgcct gacatggcac
     3841 ctcacttccc gctgcggtgg ttttcggtgg ataaaacgca aatcgctggt gaaagtctgc
     3901 atcttaacct tcaacagcgg ttgacgcgat ttgccgcaga aaatgcgccc cagttactca
     3961 acgaattaag tgacaatcaa tggctgttcc cgctgcaccc gtggcaggga gaatatcttt
     4021 tgcagcaagt gtggtgccag gcactttttg ctaaaggact tatcagagac ttaggcgagg
     4081 ccggcacgtc gtggctgccg accacctctt cccgctccct ctactgtgct accagccgcg
     4141 atatgatcaa gttctccctg agcgttcggc tgaccaactc cgtccgtact ctgtctgtga
     4201 aagaggtgga gcgaggaatg cgcctggcac gtctggcgca aaccgacggc tggcagatgc
     4261 tacaggcccg cttccctact ttccgggtaa tgcaggagga cggctgggcc gggctgcgcg
     4321 atcttaacgg caacatcatg caggaaagtt tgttttccct gcgtgaaaac ctgctgctgg
     4381 agcagccgca aagccagacc aacgttctgg tttccctgac tcaggccgca ccggatggcg
     4441 gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc
     4501 aacaggccgc ccacgcatgg gtcgatgctt actgtcagca ggtgctgaag cctctgttta
     4561 cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt cttgtgcaga
     4621 tgcttgggga tctgccggtc ggatttattt accgtgactg ccagggcagc gcgtttatgc
     4681 ctcacgcgac agagtggctc gataccattg acgaggcgca ggcggaaaat attttcaccc
     4741 gtgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact tttgccgtaa
     4801 ctgctgcgct gggtgctgcc gggctggaca gcgaagcgaa tctgatggct cgtgtacgaa
     4861 cattgctggc tgaagtgcgt gaccaggtga ctcataaaac ctgtctcaac tatgttctgg
     4921 aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat cataacgaga
     4981 acaccatcgt tgacccttcg gtgatctatt ttgatttcgc taacccgctg caggctcagg
     5041 aggtctgaat gtctggggca aacattgttc acagcggata tggactgcgc tgtgaaaaac
     5101 tcgacaagcc tctgaatctt ggctgggggc tggacaatag cgcggtgttg cactggcccg
     5161 gggagctgcc aacagggtgg ctgtgcgacg cgctggatca gatatttatc gccgcaccac
     5221 aactttcagc agtggttctt ccctggtccg aatggtgtga ggagccacag gcgctgacgc
     5281 ttttcggaca ggtacaaagc gacattatcc atcgttccgc tttctggcag ttaccgttat
     5341 ggctgagttc tccggcaaac cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga
     5401 tttatttccc gcagcgcccc ccccgtccgc agggtgaagt ttatcgtcgt tacgatccac
     5461 gcattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca gaacgtttca
     5521 ctcgctggat gaacgatccg cgcgttgagt atttctggga gcaaagtggc tcactggagg
     5581 tacagatcgc ctatctggaa cgccagctca ccagtaaaca tgcattcccg ctgattggtt
     5641 gtttcgacga tcggccgttc agttatttcg aaatctactg ggcggcggaa gaccgcattg
     5701 gtcgccacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt ggtgaacagc
     5761 aatggcgcgg agcccactat gtgcaaagct ggctgcgcgg ggtgacacat tacctgctgc
     5821 tgaatgagcc acgtacgcag cgcacagtac tggagccacg caccgataac cagcgcctgt
     5881 tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc ccacataaac
     5941 gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa
     6001 tcacaaagac tgggatttgg tcaaccgccg cctggtggca aaaatgttgt ctgagctgga
     6061 gtatgagcag gttttccacg cggaatctca gggcgatgac cgctactgca ttaacctgcc
     6121 gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctct ggattgatgc
     6181 tcaaactctg cgctgcgcgg acgagccagt actggctcag acgctgctga tgcagctaaa
     6241 gcaggtactg tcaatgagcg atgcaaccgt tgctgagcat atgcaggatt tgtatgccac
     6301 gctgctgggc gacctgcaac tactgaaagc ccgtcgcggg ctgagcgcca gtgacctgat
     6361 taatcttaat gccgaccgcc tgcaatgcct gctgagcggt catcctaaat tcgtttttaa
     6421 taaaggtcgc cgtggctggg gtaaagaggc gctggaacga tatgcgccag agtatgccaa
     6481 caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc gctgtgataa
     6541 cgagatggat attcatcagt tgttgacggc cgcaatggat ccgcaggagt ttgcccgctt
     6601 cagtcaggtc tggcaggaaa acggactgga tcataactgg ctgccgctgc cggtacatcc
     6661 gtggcagtgg cagcaaaaaa tcgctaccga cttcatcgct gattttgccg aaggcaggat
     6721 ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac
     6781 caacgccagt cgccggggcg ggctggatat caagctgccg ctgaccatct acaacacctc
     6841 atgctaccgg gggatccctg gcagatacat cgctgccgga ccactggctt cacgctggct
     6901 acaacaggtt tttgcgaccg acgccaccct agtgcaaagc ggcgcagtga tacttggtga
     6961 accggctgca ggctatgtct cccatgaagg ctatgccgcg cttgcccggg ctccctatcg
     7021 ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc tgaaaccgga
     7081 tgaaagtccg gttctgatgg caacactgat ggagtgcgac gaaaacaatc agccgctggc
     7141 aggcgcatat atagaccgct ccgggctgga cgctgaaacc tggcttacgc aactgttccg
     7201 ggtggtcgtg gttcctctgt atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca
     7261 tggacaaaat ataactctcg ccatgaaaga gggggttcca cagcgtgttc tgctgaaaga
     7321 cttccagggc gatatgcgac tggtgaaaga agagttcccc gaaatggact ctttgcctca
     7381 ggaggttcgt gatgttacat cccgcctgag tgcggactac ttaatccatg atttgcagac
     7441 gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc ttggcgtacc
     7501 tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga aaaaacatcc
     7561 acaaatgtca gagcgttttg cgcttttctc acttttcagg ccacaaatca ttcgcgtggt
     7621 gctgaacccg gtaaaactga cctggcctga tctggatggc ggcagccgca tgctgccgaa
     7681 ttaccttgag gatctgcaaa atccgctgtg gctggtaact caggaatatg aatcatgaaa
     7741 aaaagtgtcg attttattgg tgtagggaca gggccattta atctcagcat tgctgcgtta
     7801 tcacatcaga tcgaagaact ggactgtctc ttctttgatg aacatcctca tttttcctgg
     7861 catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa agatctggtc
     7921 agtgctgttg cacctacaaa tccctacagt tttgttaact atctggtgaa gcacaaaaag
     7981 ttctatcgct tccttacaag cagactacgt acagtatccc gtgaagagtt ttctgactac
     8041 ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt tgaaaacatt
     8101 gatttcgata aaaaacgtcg attgtttctg gtgcaaacca gccagggaga atattttgcc
     8161 cgcaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt gaagcatatg
     8221 acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct tagtggaaaa
     8281 cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa tgcattacgc
     8341 ggggaatggg gagaagcggc ggaaataaac tgggtctccc ggcgtaataa ttttaacgca
     8401 ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc aggcttctcc
     8461 ggactggagg aagatattcg ccatcagtta ctggatgagc agaaaatgac atcggatggc
     8521 atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt tgaagttctg
     8581 agaaaaccaa gaaatatccg tctgctaccc agccgctcgg taacaactct ggaaagtagt
     8641 ggtcctggct ggaagttgct gatggagcat catctggatc agggcaggga gagcctggaa
     8701 agtgatgtgg tgattttcgc cacaggttac cgttctgcgt tgccacaaat acttccctca
     8761 ctgatgcccc tgatcaccat gcacgataag aacaccttta aagtgcgtga tgacttcact
     8821 ctggaatgga gtggcccgaa agagaacaac atcttcgtgg tcaacgccag tatgcaaacc
     8881 catggcatcg ccgaacccca gctcagcctg atggcatgga gatctgcacg tattcttaat
     8941 cgtgtaatgg gacgtgattt attcgatctc agtatgccgc ccgccctgat tcagtggcgc
     9001 agcggcacct agggaaaacg cagccggagg ctgcttcttt aactcgctac acagcatctt
     9061 tgggctgatt ttttccgccc gtatggagga ataatgatga taagcaaaaa gtatacgctt
     9121 tgggctctca acccactgct tcttaccatg atggcgccag cagtcgctca acaaaccgat
     9181 gatgaaacgt tcgtggtgtc tgccaaccgc agcaatcgca ccgtagcgga gatggcgcaa
     9241 accacctggg ttatcgaaaa cgccgaactg gaacagcaga ttcagggcgg caaagagctt
     9301 aaagacgcac tggctcagct gatccctggc cttgacgtca gcagccggag ccgcaccaac
     9361 tacggtatga atgtgcgtgg ccgcccgctg gtcgtactgg ttgacggcgt gcgtctcaac
     9421 tcttcacgta ccgacagccg acaactggac tctatagatc cttttaatat cgaccatatt
     9481 gaagtgatct ccggtgcgac gtccctgtac ggcggcggca gtaccggtgg cctgatcaac
     9541 atcgtgacca aaaaaggcca gccggaaacc atgatggagt ttgaggctgg caccaaaagt
     9601 ggctttagca gcagtaaaga tcacgatgaa cgcattgccg gagctgtctc cggcggaaat
     9661 gagcatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg gtttgacggt
     9721 aacggcgatg ccaccttgct tgataacacc cagaccggcc tgcagtactc cgatcggctg
     9781 gacatcatgg gaactggtac gctgaacatc gatgaatccc ggcagcttca gttgatcaca
     9841 cagtactata aaagccaggg cgacgacgat tacgggctta atctcgggaa aggcttctct
     9901 gccatcagag ggaccagcac gccattcgtc agtaacgggc tgaattccga ccgtattccc
     9961 ggcactgagc ggcatttgat cagcctgcag tactctgaca gcgcttttct gggacaggag
    10021 ctggtcggtc aggtttacta ccgcgatgag tcgttgcgat tctacccgtt cccgacggta
    10081 aatgcgaaca aacaggtgac ggctttctct tcgtcacagc aggacaccga ccagtacggc
    10141 atgaaactga ctctgaacag caaaccgatg gacggctggc aaatcacctg ggggctggat
    10201 gctgatcatg agcgctttac ctccaaccag atgttcttcg acctggctca ggcaagcgct
    10261 tccggagggc tgaacaacaa gaagatttac accaccgggc gctatccgtc gtatgacatc
    10321 accaacctgg cggccttcct gcaatcaggc tatgacatca ataatctctt taccctcaac
    10381 ggtggcgtac gctatcagta cactgaaaac aagattgatg atttcatcgg atacgcgcag
    10441 caacggcaga ttgccgccgg gaaggctaca tccgccgacg ccattcctgg cggctcagtc
    10501 gattacgaca acttcctgtt caacgccggt ctgctgatgc acatcaccga acgccagcag
    10561 gcatggctca acttctccca gggcgtggag ctgccggacc cgggtaaata ctatggtcgc
    10621 ggcatctatg gtgctgcagt gaacggccat cttcctctaa caaagagtgt gaacgtcagc
    10681 gacagcaagc tggaaggcgt gaaagtcgat tcttatgagc tgggctggcg ctttactggc
    10741 aataatctgc gcacccaaat cgcggcctac tattcgattt ctgataagag cgtggtggcg
    10801 aataaagatc tgaccatcag cgtggtggac gacaaacgcc gtatttacgg cgtggaaggt
    10861 gcggtggact acctgattcc tgatactgac tggagtaccg gagtgaactt caacgtgctg
    10921 aaaactgagt cgaaagtgaa cggtacctgg cagaaatacg atgtgaagac agcaagccca
    10981 tcaaaagcga cagcctacat tggctgggca ccggacccgt ggagtctgcg cgtgcagagc
    11041 accacctcct ttgacgtgag cgacgcgcag ggctacaagg tcgatggcta taccaccgtg
    11101 gatctgctcg gcagttatca gcttccggtg ggtacactca gcttcagcat tgaaaacctc
    11161 ttcgaccgtg actacaccac tgtctggggg cagcgtgcac cactgtacta cagcccgggt
    11221 tacggcccag cgtcactgta cgactacaaa ggcaggggcc gaacctttgg tctgaactac
    11281 tctgtgctgt tctgaccggt attcctttac aacaaaggta cgctgatatc aacatggccg
    11341 ctgacagcca agttgatatc atataataca cgacataatc tgtagtcagg gaggatag
//
LOCUS       DAMZII010000151        11308 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZII010000151 DAMZII010000000
VERSION     DAMZII010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.151"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            276..857
                     /gene="mdaB"
                     /locus_tag="POA77_004024"
     CDS             276..857
                     /gene="mdaB"
                     /locus_tag="POA77_004024"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDI6367382.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            888..1202
                     /locus_tag="POA77_004025"
     CDS             888..1202
                     /locus_tag="POA77_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6367383.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1250..3142)
                     /gene="parE"
                     /locus_tag="POA77_004026"
     CDS             complement(1250..3142)
                     /gene="parE"
                     /locus_tag="POA77_004026"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6367384.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3171..3752)
                     /gene="yqiA"
                     /locus_tag="POA77_004027"
     CDS             complement(3171..3752)
                     /gene="yqiA"
                     /locus_tag="POA77_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDI6367385.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3752..4579)
                     /gene="cpdA"
                     /locus_tag="POA77_004028"
     CDS             complement(3752..4579)
                     /gene="cpdA"
                     /locus_tag="POA77_004028"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDI6367386.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADKRFQPDTASEGY"
     gene            complement(4604..5026)
                     /locus_tag="POA77_004029"
     CDS             complement(4604..5026)
                     /locus_tag="POA77_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDI6367387.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5027..5656)
                     /gene="nudF"
                     /locus_tag="POA77_004030"
     CDS             complement(5027..5656)
                     /gene="nudF"
                     /locus_tag="POA77_004030"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDI6367388.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5861..7342
                     /gene="tolC"
                     /locus_tag="POA77_004031"
     CDS             5861..7342
                     /gene="tolC"
                     /locus_tag="POA77_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDI6367389.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7490..8161
                     /locus_tag="POA77_004032"
     CDS             7490..8161
                     /locus_tag="POA77_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDI6367390.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8167..9327
                     /locus_tag="POA77_004033"
     CDS             8167..9327
                     /locus_tag="POA77_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI6367391.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(9365..10153)
                     /gene="ygiD"
                     /locus_tag="POA77_004034"
     CDS             complement(9365..10153)
                     /gene="ygiD"
                     /locus_tag="POA77_004034"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDI6367392.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10296..11069
                     /gene="zupT"
                     /locus_tag="POA77_004035"
     CDS             10296..11069
                     /gene="zupT"
                     /locus_tag="POA77_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDI6367393.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11127..>11308)
                     /locus_tag="POA77_004036"
     CDS             complement(11127..>11308)
                     /locus_tag="POA77_004036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367394.1"
                     /translation="GKRMFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARN
                     KYLNSTREETAEKVE"
BASE COUNT         2799 a         3053 c         2805 g         2651 t
ORIGIN      
        1 acacgttgat attttcatct tttacttatt caaagccata ttttcaatcc caaaaaaaac
       61 cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt atgaggaatg gctatcagta
      121 ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagctagat cagacttttg
      181 ctaaattcgc aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac
      241 tatagtcgac aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg
      301 gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgcgg
      361 atggcacact gcgcgacctc gggcatgatg tccgcatcgt tcgcgccgac agcgactacg
      421 atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg cagatgccag
      481 gctggtggat gggcgcgccg tggacagtga aaaaatatat tgatgatgta ttcaccgaag
      541 gtcatggaac gctgtatgcc agcgatggcc gtacccgcaa agatccgtca aaaaaatacg
      601 gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg aacgcaccaa
      661 tggaagcctt caccgaaaaa gatcaattct tccacggcgt tggcgtcgac ggtgtgtatc
      721 tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca tttatcgcta
      781 atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc aagcatcttg
      841 tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa
      901 tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg
      961 ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg
     1021 attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc
     1081 agtgggaaag catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca
     1141 gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg catcctgcaa cctgggattt
     1201 aaacctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt taaacctcaa
     1261 tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag cgtttcttcg
     1321 ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact
     1381 gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc gggttcattt
     1441 cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt aattgctcaa
     1501 gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg agatcaatac
     1561 ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt
     1621 ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga
     1681 ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga tcgataccga
     1741 tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa gaaacttccc
     1801 aggtgttaag gatcttacct ttcagtggca tgatcgcctg atattcgcga tcgcgcgcct
     1861 gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa cagttcggta cggttaagat
     1921 cctgcgcggt acagtcagcc agtttgccag gcagtgctgg gccgctggtc agctttttgc
     1981 gcaccacttt tttagccgca cgcatacggc gctgggcgct ggaaatcgcc atctccgcca
     2041 gcagctccgc cgcctgaacg ttctgattca gccacaggat aaaggcatct ttcaccacgc
     2101 ctgaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttggtctgc ccggcaaact
     2161 gcggatcctg cattttcacc gacagaacat aggcgcagcg atcccagata tcttccgccg
     2221 acagcttcac gccgcgcggc agaatgttgc ggtattcaca gaactcacgc atcgcgtcca
     2281 acaggccctg acgcagacca ttaacatggg taccgccctg catcgttggg ataaggttga
     2341 cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc cagtccacag
     2401 cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc ggtaaaccgt
     2461 ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc tgttcggtgt
     2521 tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtactgct ttggccttca
     2581 gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc
     2641 agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct
     2701 gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg cgcacgttaa
     2761 cttctacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc aggccgccag
     2821 agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa agaatcagtt
     2881 caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa
     2941 taacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg tgacccgcca
     3001 gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg cgagtggtat
     3061 cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat
     3121 cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg tcagagatgg
     3181 tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg
     3241 ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa
     3301 tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc ttccagcggg
     3361 tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata ttgctgcccg
     3421 gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg gcgtaccgcc
     3481 gggtttacca ccactgcggg cagcataaaa cattgcgaca accaggtggc gtaatatccc
     3541 cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac aatggattcc
     3601 aacagctctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat ctcaacgtca
     3661 gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg cggcgagctg
     3721 ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt
     3781 ggaaacgttt gtccgccagg cgatgcacct cggtggtcag cgtgccatca gcatgtaact
     3841 cgagagtacg ccagccgggc gcgatggtgt ccagcgtaaa gttggaacag tgcggcttaa
     3901 actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg agatccagct
     3961 cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat
     4021 ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc gcaggtagcg
     4081 gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt ttacgttcca
     4141 gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc tggctatcca
     4201 gcaacaggat ttgccattgc tcaccaataa acacgcgctt cgccggggag atacccgcat
     4261 cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc cagacgcagg
     4321 gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag gattgatcct
     4381 gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctgatgtgga cgaatcgcct
     4441 ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg
     4501 caaacaggtg agtatcggta atttgtaaaa tcctgactct ggcctcacca gccagaggaa
     4561 gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctagtaa accggaatcg
     4621 ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactgg ttaatttgat
     4681 gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct ttgaagcgaa
     4741 aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga cgcaccgtca
     4801 ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc accagggtag
     4861 tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc tgatagctta
     4921 cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa aagttcatct
     4981 cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat gcccactcat
     5041 tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac gctgcgttgt
     5101 cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca tgaacgcgaa
     5161 tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc
     5221 ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa cttaacaccg
     5281 gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt
     5341 ccacactttc accctcttca atcatcccgg caaccatctc cagtagccag ggggtttcgc
     5401 tggtgtcgta cgcggcaatc cgaatctgct caatcagcac aacttcatca cgcactgggt
     5461 caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt acctcatgac
     5521 tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa aaaaagccgc
     5581 gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta acgggcaggt
     5641 tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg tgaatttcag
     5701 cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta acgccctatg
     5761 gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat aattttacag
     5821 tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat tgctccccat
     5881 tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca
     5941 agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga
     6001 tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg
     6061 tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac
     6121 cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac
     6181 gctacaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc agcaaacctt
     6241 gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta
     6301 tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa
     6361 cgtgggcctg gtagcgataa ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct
     6421 ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat
     6481 caccggtaac tactatccag aactggcagc gctgaatgtc gaaaacttta aaaccgacaa
     6541 accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca
     6601 ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt
     6661 accgacgctg gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa
     6721 aactcgtggt gccgctggta cccagtatga cgacagcaat atgggccaga acaaagtggg
     6781 cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc
     6841 ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta gcgtcgtgca
     6901 aaccgtacgt tcctccttca acaacattaa tgcatctatc agtagcatta acgcctacaa
     6961 acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg
     7021 tacgcgtacc attgttgatg tgttggatgc aaccaccacg ctgtacaacg ctaagcaaga
     7081 gctggcaaat gcgcgttata actacctgat taatcagctg aatattaagt cagccctggg
     7141 tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac
     7201 taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg atggttatgc
     7261 gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca ccagtaacgg
     7321 tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct gaacgtaagg
     7381 caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca atttcgacca
     7441 gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa tgaaacggac
     7501 aaaatccata cgccacgcat cgttccgcaa aaactggagc gcgcgccatc tgacaccagt
     7561 cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg atgaaacagt
     7621 gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg
     7681 taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga aatacgccac
     7741 ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac cagcccaggc
     7801 tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc
     7861 gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat ttgcacagca
     7921 gccgctgttc tcctcgaaaa acccagccag cccggcttac ggtaaatata ccgacgcgac
     7981 gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga agacagccat
     8041 ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg aatctgttgc
     8101 caaacaaagc actatgcagc gtagcgcaac tggcacctct tctcgttcaa tgggtggctg
     8161 ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa agcccacgaa
     8221 tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga tgcttactac
     8281 aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact gcaccagatg
     8341 tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa attccgcatt
     8401 ccaaaacaca cctggagttt tgtgcgtcag tcatggctga ctcaccagcc atcgctttat
     8461 tcgcgtcttg atctggcatg ggatggcact ggcgaaccta aactgctgga aaataacgcc
     8521 gatacaccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct ggaagatcag
     8581 cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca agaaaagcta
     8641 atcgatcgct ttgtcgagtt gcgtgaacag tatggcttcc agttgctgca tctcacctgc
     8701 tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg cgcgacggaa
     8761 gctgaaatcg ctaccgagtt cctctacatt gacgatatcg ggttaggtga aaaaggtcag
     8821 ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc atgggaattt
     8881 atgttgcgtg agatgttctc caccaagctg gaggatgcag gcgtacgctg gctggaaccg
     8941 gcgtggaaga gcattatctc caacaaagcg cttctaccgc tactgtggga gatgttcccg
     9001 aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca aatggaaaaa
     9061 tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc
     9121 aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt tcagcaattc
     9181 cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct ggtgaacgat
     9241 caacctgccg gaattggcat tcgtgaagac cgtgcgttga tcacccagga tatgtctcgg
     9301 ttttatccac atatttttat tgagtaatca atgatgccgg atggcattcg ccatccggta
     9361 attgttaacc tatctgcacc gacagcatac tcaggctgcc catttctata ccatcgaccg
     9421 gaatggtaat tggctcctgc ccatcccacg cacctaagac atacaacaac ggcagatagt
     9481 gctccggcgt tgggttcgat aacgcgccac cttcatggtc gaggtaattc accagaggat
     9541 gctgctctac cgccccttgc cacgtcagat tcgctttcac atactcatta aacgacatcg
     9601 cccaaggata tggtgaacta tcaccgtgcc acttcactgt acgcaggtta tgcaccacgt
     9661 tgccactggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg cgccccattt
     9721 cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc gggatatcgg
     9781 cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc caggcttctt
     9841 tatccagcgt caccgggacc ggcgctaaca gctcaaccag atgctgtgcc agcacaggcg
     9901 aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa
     9961 tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa
    10021 ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag ctgcgggtat
    10081 acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac
    10141 gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg
    10201 cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga
    10261 tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca
    10321 tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct
    10381 cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg ctcatctcat
    10441 taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg
    10501 gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgcgc
    10561 atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag
    10621 ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg
    10681 tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc gctttgcaca
    10741 atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg tctaaacgca
    10801 ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt gtgctggcgt
    10861 ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg gcggcggttg
    10921 caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc
    10981 ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag
    11041 tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc agcacatgga
    11101 caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc ttctctcgta
    11161 ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga
    11221 caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa tacaatccag
    11281 taccaggcga taaacatcct tttaccct
//
LOCUS       DAMZII010000152        11182 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZII010000152 DAMZII010000000
VERSION     DAMZII010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.152"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            76..180
                     /locus_tag="POA77_004037"
     CDS             76..180
                     /locus_tag="POA77_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDI6367395.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            491..2689
                     /gene="speF"
                     /locus_tag="POA77_004038"
     CDS             491..2689
                     /gene="speF"
                     /locus_tag="POA77_004038"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDI6367396.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2686..4005
                     /gene="potE"
                     /locus_tag="POA77_004039"
     CDS             2686..4005
                     /gene="potE"
                     /locus_tag="POA77_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDI6367397.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4061..5701)
                     /gene="pgm"
                     /locus_tag="POA77_004040"
     CDS             complement(4061..5701)
                     /gene="pgm"
                     /locus_tag="POA77_004040"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDI6367398.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5727..6272)
                     /gene="seqA"
                     /locus_tag="POA77_004041"
     CDS             complement(5727..6272)
                     /gene="seqA"
                     /locus_tag="POA77_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDI6367399.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6457..7221
                     /gene="ybfF"
                     /locus_tag="POA77_004042"
     CDS             6457..7221
                     /gene="ybfF"
                     /locus_tag="POA77_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6367400.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7361..7654
                     /gene="ybfE"
                     /locus_tag="POA77_004043"
     CDS             7361..7654
                     /gene="ybfE"
                     /locus_tag="POA77_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDI6367401.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7794..8324
                     /gene="fldA"
                     /locus_tag="POA77_004044"
     CDS             7794..8324
                     /gene="fldA"
                     /locus_tag="POA77_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDI6367402.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8534..8620
                     /gene="uof"
                     /locus_tag="POA77_004045"
     CDS             8534..8620
                     /gene="uof"
                     /locus_tag="POA77_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDI6367403.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8613..9059
                     /gene="fur"
                     /locus_tag="POA77_004046"
     CDS             8613..9059
                     /gene="fur"
                     /locus_tag="POA77_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDI6367404.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9143..9469)
                     /locus_tag="POA77_004047"
     CDS             complement(9143..9469)
                     /locus_tag="POA77_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367405.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9519..10925)
                     /gene="chiP"
                     /locus_tag="POA77_004048"
     CDS             complement(9519..10925)
                     /gene="chiP"
                     /locus_tag="POA77_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDI6367406.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2713 a         2765 c         2787 g         2917 t
ORIGIN      
        1 cttcttcaca gattatagcc atttcatgga tagaataact ctaccttcaa ctgacacagc
       61 aagaggtaaa ggtaaatgga aaataataac cgcttaatgc ctcatataag gcggacaacc
      121 catatcatga agtttgccca tcgtaatagt tttgactttc atttctttaa tgcccgctag
      181 tcttctgact aaagggcacc ccaacgtaca ggtctccctg actttaagca ttacaggtta
      241 atacctgtat tcctcggtgc tcatatactg ctaacccttt taaactctaa ataattcgag
      301 tcgcagcact tgcaacttga ggtatgacga gtatagccag ttaccgggct ggtctgggtt
      361 attgcatctg caaaaagcaa actactgatt tatttatcag cggtggagct ttgccttttt
      421 tccggcgtga tcgatttctc ctttgagaaa ttgaggacct gctattacct gaaataaaga
      481 gatgaacaaa atgtcagaat taaaaattgc cgttagtcgt tcttgcccgg attgtttttc
      541 cactcatcgt gcatgcgtga atatagacga aagtaattat attgacgttg ccgccattat
      601 tttatcagtc agtgatgttg aacgtggaaa actcgatgaa atagacgcta ctggctatga
      661 cattcctgtt tttattgcaa cggaaaatga agaacgtgta cccgcagaat atctgccacg
      721 tatttctggt gtcttcgaac attgcgaatc acgtaaagag ttttatggac gtcagttaga
      781 aaccgctgcc agccattatg aaacgcaact gcgcccgccc ttcttccgcg cactggtcga
      841 ttatgttaat cagggcaata gtgcttttga ctgccctggt catcaggggg gtgagttttt
      901 ccgtcgccat ccggcaggca atcagttcgt ggaatacttt ggcgaaatgc tcttccgctc
      961 tgacttgtgt aatgctgacg tagcaatggg cgatctgctg attcatgaag gtgcgccatg
     1021 tattgcacaa cagcatgcgg ctaaagtctt taatgccgat aaaacctatt tcgttttaaa
     1081 cggtacttca tcttctaata aagtggtctt aaacgcattg ctgacgccgg gcgatctggt
     1141 gttgtttgac cgcaataacc ataaatctaa ccaccacggt gcgttgctac aggctggcgc
     1201 gacaccggtt tatctggaaa ccgcacgtaa cccgtatggt tttatcggtg gtattgacgc
     1261 tcactgtttt gaagaaagtt acctgcgtga gctgattaca gaagttgcac cgcagcgggc
     1321 aaaagaggct cgtcctttcc gcctcgccgt tattcagcta ggcacttacg acggtacgat
     1381 ttataacgcc cgtcaggtgg tggataaaat tggtcatctg tgtgactaca tcctctttga
     1441 ctccgcatgg gtcggctatg aacagtttat tccgatgatg gctgactgtt cgccgctgtt
     1501 gctggatctt aatgagaacg atccgggtat tctggttacg caatccgtac ataaacagca
     1561 ggctggtttt tctcaaactt cacaaatcca taaaaaagac agccacatca aagggcaaca
     1621 gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg atgcacgcct ccaccagccc
     1681 gttctatccg ctgtttgccg cactggatat caacgccaaa atgcatgaag gtgtcagcgg
     1741 tcgtaatatg tggatggatt gtgtggtaaa tggcattaat gcccgcaaac tgatcctcga
     1801 taactgtcag catattcgtc cgttcgtacc tgaactggtg gatggtaaac cctggcagtc
     1861 gtatgaaaca gcgcaaattg cggttgatct gcgcttcttc cagtttgtac caggggaaca
     1921 ctggcattct tttgaaggct atgcagagaa tcaatacttt gtcgatcctt gcaaactgtt
     1981 gctgacaacc ccaggtattg atgcacgtaa cggcgaatat gaagcgttcg gtgtacccgc
     2041 gacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat gcgatcttaa
     2101 ctccatcctc ttcctgctga ctccggcaga agatatggcc aagcttcagc aacttgttgc
     2161 cctgctggta cgcttcgaaa aactgcttga gtccgacgcg ccattagcag aagtgctgcc
     2221 ttccatctac aaacagcatg aagagcgcta cgccggttat accctgcgtc agttgtgtca
     2281 agaaatgcat gatttgtatg cccgtcacaa cgtgaaacaa ctgcaaaaag agatgttccg
     2341 taaggaacac ttcccacgcg taagcatgaa tccgcaagaa gccaactacg cctatttacg
     2401 cggtgaagtg gagctggttc gtctgccgga tgcagaaggc cgtatcgctg ccgaaggtgc
     2461 gcttccttat cctccgggtg tgctgtgcgt tgttccgggt gaaatctggg gtggtgctgt
     2521 tctgcgttac ttcagcgctc tggaagaagg gatcaacctg ttgccaggtt ttgcaccgga
     2581 gcttcagggt gtctatatcg aagaacatga tggtcgtaag caagtttggt gctatgtcat
     2641 caagcctcgt gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa
     2701 tcgaacaaaa tgggcgtcgt tcagttaacc atactgacga tggtcaacat gatgggctcc
     2761 ggtatcatca tgctgccgac aaagcttgcc gaagttggga caatttcaat tatctcctgg
     2821 ctggtgacag ccgtaggctc aatggcactg gcatgggcat tcgcaaaatg cggtatgttc
     2881 agccgtaaat caggcggtat gggcggttat gcggagtatg ctttcggtaa atccggtaac
     2941 tttatggcga actataccta cggcgtctcg ctgctgattg ctaacgtcgc gattgctatt
     3001 tcggcggttg gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt
     3061 cttgcgacca tcggggtgct gtggatttgt accgtggcta actttggtgg tgcgcgcatt
     3121 accgggcaaa tcagtagcat taccgtgtgg ggggtcatta ttccggtggt aggcctgtgc
     3181 attatcggct ggttctggtt tagcccgacg ctttacgttg attcctggaa tccgcatcat
     3241 gcaccgttct tcagtgcagt aggttcttcc atcgctatga cgctgtgggc ttttctcggt
     3301 ctggagtctg cgtgtgcgaa tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc
     3361 gcggtactcg gcggtacgtt aggtgcggcg gtgatttata tcgtctccac caacgtgatt
     3421 gcagggattg tgcctaatat ggagctggca aattcaacgg caccatttgg cctggccttc
     3481 gcacagatgt tcacaccaga agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc
     3541 tgcggttcgc tgcttggctg gcagttcacc attgcccagg tgtttaaatc ttcatctgat
     3601 gaaggctact tccctaaaat tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga
     3661 atgttgacca ttgtgattat ccagagcggg ttggcgctga tgacgattag cccgtcgcta
     3721 aacagtcagt tcaacgtgct ggttaacctg gccgtggtga ccaatatcat tccgtatatt
     3781 ctgtcaatgg cggcattagt cattattcag aaggtcgcta atgtgccccc ctcaaaagcg
     3841 aaagttgcaa actttgttgc ttttgttggc gcgatgtata gcttttatgc gctgtactca
     3901 tccggggaag aagccatgct gtacggttcc atcgtgacct tccttggttg gacactgtat
     3961 ggtctggtat caccacgctt tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa
     4021 aagggcgatc ttgcgaccgc ccttttttta ttaaatgtgt ttacgcgttt ttcagaactt
     4081 cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg atgttcttca ccgaggaagc
     4141 tttcgcagta gatcttatat gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt
     4201 tgtcagtcat cactttcaga ccgccaatag aagcaccgtt gcccggagca gcagtcaggc
     4261 gcgcggtgat cgggtcacct gccagggtgc tggcgctcac catttccgga gacagcttag
     4321 acagcgccgc tttttgtgcg gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac
     4381 caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga
     4441 tttccgccgc cagcagacac ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt
     4501 cgaaacgcag gaaggaagcc cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga
     4561 acagaccatc aacaaaccat ttgaaaccta ccggtacttc taccagctta cggcccaagt
     4621 cattgaccac acggtcgatc atcgcagaag aaaccagcgt tttaccgaca gcaacatctt
     4681 tgccccactg cggacgatgc tggaacaggt agttgatcgc caccgccagg tagtggttcg
     4741 gattcatcaa acctgccgga gtgacgatac cgtgacggtc ataatccggg tcgttagcaa
     4801 acgccagatc gaacttatca cgcagtgcca gcagacccgc catcgcacac tcggaggagc
     4861 agtccatacg gatcgcaccg tctttatcga ggtgcataaa gcggaaggtt tgatcgacct
     4921 gatcgttaac gatggtcagg ttgaggttgt aatactcgcc aatgcgcttc cagtattcga
     4981 taccggaacc gcccaacgga tcaacgccca gcgtcaggcc cgctttctga atcgccgcca
     5041 tatcaacgat atcggccagc ccttccacga acggctgcac cagatcctgc tctttcacat
     5101 gaccggatgc catcgcttcg tcgagggaga tacgcttcac gcctttcagg ccatcggcca
     5161 tcagtgcgtt ggccctgtct tccaccactt tggtgacgtt agtatcagcc gggccaccat
     5221 ttggcggatt gtacttgata ccaccatctt ccggcgggtt atgggacggt gtaatcacga
     5281 taccgtctgc cagcgggcca ccttttttat tgtgaaccag gatggcattg gaaacggcag
     5341 gcgtcggagt gaagccattg ttttcctgca caatgacatc aacgccgttc gctgccagca
     5401 cttccagtac ggaaatgaac gcaggctcgg acagggcgtg agtatcttta cccacatagc
     5461 aagggccagt gatgccgttt ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa
     5521 tgtgcggctc gttaaagctg tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt
     5581 tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac ataatattgc gccgtcagtt
     5641 gggcgacgtt aatcaaatca ctctgttgtg caggttggcc tgcacgattg tggattgcca
     5701 ttgctttgtc ctttgtctgc aacgttttag atagttccgc aaaccttctc aatcaattcc
     5761 gccgggaatt gcatcgactg catgatgtgt tcgatcatgc tgcatttacg gccggtgttg
     5821 gtgttggtga tcacccaata cggcgtgcct ggcacatgtt tcggcttggt ctgattacca
     5881 tttttcagca gcgtttgttc atctgccgca aagtaaacgc gtgtacgacc gtgcaacgat
     5941 tccgttgctt cggcaaacgc ctgggcgtca agagaatata gtgtagacaa cagcagcata
     6001 aagcgattga ccgctcgctt ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt
     6061 gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt cgacgatagc aggtgacgca
     6121 acgcgaacct ctttcgtcac cggagcagca ggctgtgatg cggcggaaaa tttcaacata
     6181 cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag tgtggctggc aatatagctg
     6241 tagagttcat catcaacttc aatcgttttc atcttaatcc agtgcagtgt cttagctgaa
     6301 tacaagtcgc caggagtata tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg
     6361 cagaaaaact cggaataaac cttatttgtt gcagccctgt ggcacaatgg tcaacataat
     6421 accctaaccc gacaacgcga aaaaaagaac tttgccatga aattgaatat ccgcgcgcaa
     6481 actgcacaaa accagcacaa taattctccc atcgttcttg tccatggtct gtttggtagc
     6541 ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg atcacaatat catccaggtt
     6601 gatatgcgta accacggtct ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg
     6661 caggatcttg ttgataccct ggatgcactg cagatcgaca aagcaacatt tatcggtcac
     6721 tccatgggcg gtaaagcggt aatggcactt actgcactgg cccccgatcg catcgataaa
     6781 ctggtggcga tcgatatcgc accggtcgac tatcacgtac gccgtcatga tgagattttc
     6841 gcagctatca acgcggtcag tgaatcggac gcacaaactc gccagcaagc agcagcaata
     6901 atgcgccagc atcttaatga agaaggggtg attcagtttc tgttgaaatc ttttgttgac
     6961 ggggagtggc gctttaacgt gccggtattg tgggatcagt atccgcatat tgtgggttgg
     7021 gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat
     7081 gttagcgagc agtaccgtga tgatttactg gcacaatttc cacaggcacg ggcgcatgtg
     7141 attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc
     7201 cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg
     7261 cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat ggcgcgctat ccatccgggc
     7321 agaggcctgg ctgattgttt cccccgaagt caccaagatc atggccaaag aacaaacgga
     7381 ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc
     7441 gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt
     7501 acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa gctgtcgagg cgctgaacga
     7561 gctggcggaa tcgcgcaata tgagccgtag cgaactgatc gaagagatgt taatgcagca
     7621 actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc agtttcatgt agcacagtgt
     7681 gcagtcctgc tcgtttgctg attccgcaag actgcctgtt ctgctatgat tgcctttatc
     7741 cgtgggcaat tttccacccc catttcaata agtttcaaga ggttatttca ctcatggcta
     7801 tcactggcat ctttttcggc agcgacaccg gtaataccga aaatatcgca aaaatgattc
     7861 aaaaacagct tggtaaagac gttgccgatg tccatgacat tgcaaaaagc agcaaagaag
     7921 atctggaagc ttatgacatt ctgctgctgg gcatcccaac ctggtattat ggcgaagcgc
     7981 agtgtgactg ggatgacttc ttcccgactc tcgaagagat tgatttcaat ggcaaactgg
     8041 ttgcgctgtt tggttgtggt gaccaggaag attacgccga atatttctgc gatgcattgg
     8101 gcaccatccg cgacatcatt gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg
     8161 cgggctatca tttcgaagca tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg
     8221 ctatcgacga agaccgtcag ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac
     8281 agatttctga agagttgcat ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga
     8341 ctcatgtcta cgccgtatta atagataatg ccaatcaaaa taattgctac aaatttgtaa
     8401 cttttgctgt tgtacctgta caatgtcccg gtgttcaagt ggccttgccg ttgtaaatgt
     8461 aagctgtgcc acgtttttat taacaatatt tgccagggac ttgtggtttt catttaggcg
     8521 tggcaattct ataatgatac gcattatctc aagagcaaat tctgtcactt cttctaatga
     8581 agtgaaccgc ttagtaacag gacagattcc gcatgactga taacaatacc gccctaaaga
     8641 aagctggcct gaaagtaacg cttccacgtt taaaaatcct ggaagttctt caggagccgg
     8701 acaaccatca cgtcagtgcg gaagatttat acaaacgtct gatcgatatg ggtgaagaaa
     8761 ttggtctggc tacggtatat cgcgtactga accagtttga cgacgctggt atcgtcaccc
     8821 gccacaattt tgaaggcggt aaatccgtat ttgaactgac acagcaacat caccacgatc
     8881 acctgatttg cctcgactgc ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc
     8941 gtcagcgtga aattgccgca aaacatggca ttcgcctgac taaccacagt ctctatcttt
     9001 acggtcactg tgccgaaggc gattgccgcg aagatgagca cgcgcacgaa ggcaaataag
     9061 ccagcctgaa cgagaaaagc caacctgcgg gttggcttgt ttatacaagg gaaataagaa
     9121 aagcgagagt tacagctctc acttatttgt tattactgcg tatttcctgc cagaccttat
     9181 cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga cactgctgat
     9241 aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc tctttcttca
     9301 gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt gcggtgttga
     9361 tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt gattgcgcac
     9421 aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata gcaaattcct
     9481 caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt gaatggtgcg
     9541 attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc gccgccccag
     9601 cttgggatgt cagagtggtt gtcgtattcg gtgaagtgca gtttgaacat cgtgcctttg
     9661 gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc ttcaatagtc
     9721 cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt agcatcccat
     9781 gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt caggtcatac
     9841 atcgcaccga agaagaccgc tttttcgccg ttggcgttga agtcggaacg gttatcccac
     9901 cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg caggaagtat
     9961 ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac tacgtcagcc
    10021 gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag atcgttgacg
    10081 ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt taacgggcta
    10141 ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata cccttccgcc
    10201 tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc cccaagggag
    10261 tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat ttccagatgc
    10321 cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc agcatcgccg
    10381 taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa gctccagtgc
    10441 ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca aaccggaccg
    10501 tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc ggaccagtct
    10561 tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga gctgtcgccg
    10621 ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat atcagcagca
    10681 taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt ggttttgtat
    10741 ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata tataccgccg
    10801 gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc cataaagccc
    10861 gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc actaaacgta
    10921 cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac agcgaaaaaa
    10981 taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca gactattttt
    11041 cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga gcaaccgcac
    11101 atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt gcagaagaat
    11161 aaaagcgtga atttccagca ag
//
LOCUS       DAMZII010000153        11181 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZII010000153 DAMZII010000000
VERSION     DAMZII010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.153"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..360
                     /locus_tag="POA77_004049"
     CDS             <1..360
                     /locus_tag="POA77_004049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367407.1"
                     /translation="GWNASTPTTSLSWYNAYNKTKHDRSGAFHFSTLENVMDAVAACV
                     VMYCVKYGPFSLLEANTSLSTIVNQNFSISLDNSNPASYYIPEIELPPDTRTDLLIYD
                     CYRENHNKAWVTDPLVL"
     gene            complement(417..1043)
                     /locus_tag="POA77_004050"
     CDS             complement(417..1043)
                     /locus_tag="POA77_004050"
                     /inference="COORDINATES: protein motif:HMM:NF024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6367408.1"
                     /translation="MVFEPAKEFIAYCGNQYRKLSNITMSQLIDETPRYNGQMSIQEV
                     NYQWAVALLTAQHTIILELNASEADKDTPTLMGEDQRQIVERQIKQRSGQKTFRDQLL
                     KSNPTCAVTGCKLVDILDAAHINVYRNDSHNHISNGLLLRSDIHTLYDLNLCAINPIT
                     KTIHFSVEALENGYSKFEGLKINTQHNIAIKPLLTRWQLLKFQNSIDI"
     gene            complement(1441..2469)
                     /locus_tag="POA77_004051"
     CDS             complement(1441..2469)
                     /locus_tag="POA77_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6367409.1"
                     /translation="MGKSKKNRAASTNQIQHKSQTTAEAFSFGDPIPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYSLTTQPYQFTKG
                     NVFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPSNVGGFGDVEKAANVFVRNELLPLQKRMREVNDWLEQKIL
                     RFEPYSLG"
     gene            complement(2469..4235)
                     /locus_tag="POA77_004052"
     CDS             complement(2469..4235)
                     /locus_tag="POA77_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDI6367410.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAAKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEFF"
     gene            4378..5211
                     /locus_tag="POA77_004053"
     CDS             4378..5211
                     /locus_tag="POA77_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDI6367411.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5228..6286
                     /locus_tag="POA77_004054"
     CDS             5228..6286
                     /locus_tag="POA77_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDI6367412.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSSTTGGETTPRAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDTPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            6290..6940
                     /locus_tag="POA77_004055"
     CDS             6290..6940
                     /locus_tag="POA77_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDI6367413.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATCFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            7036..7500
                     /locus_tag="POA77_004056"
     CDS             7036..7500
                     /locus_tag="POA77_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDI6367414.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYVSLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            7500..7703
                     /locus_tag="POA77_004057"
     CDS             7500..7703
                     /locus_tag="POA77_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDI6367415.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEFGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            7707..7922
                     /locus_tag="POA77_004058"
     CDS             7707..7922
                     /locus_tag="POA77_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367416.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            7903..8415
                     /locus_tag="POA77_004059"
     CDS             7903..8415
                     /locus_tag="POA77_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDI6367417.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVEQALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            8417..8794
                     /locus_tag="POA77_004060"
     CDS             8417..8794
                     /locus_tag="POA77_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367418.1"
                     /translation="MKKKVISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSFAWTGWAITL
                     VFYLLTVIAFWMIRNQIAQQVAA"
     gene            8791..9219
                     /locus_tag="POA77_004061"
     CDS             8791..9219
                     /locus_tag="POA77_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDI6367419.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELRSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGSGNAAH"
     gene            9315..9746
                     /locus_tag="POA77_004062"
     CDS             9315..9746
                     /locus_tag="POA77_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6367420.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPVLREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGTVSSVEAVAEPDEPEEVWTVKRG"
     gene            9739..10182
                     /locus_tag="POA77_004063"
     CDS             9739..10182
                     /locus_tag="POA77_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDI6367421.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRNGPEVRYAERQLLGIDSKITAVIRNKLFEFIK"
     gene            complement(10189..>11181)
                     /locus_tag="POA77_004064"
     CDS             complement(10189..>11181)
                     /locus_tag="POA77_004064"
                     /inference="COORDINATES: protein motif:HMM:NF024700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6367422.1"
                     /translation="AGVVNGYQFKKHKNFNARDIMVEGSGFLQWLSVYTLALSEEFNV
                     ILLDEPDAHLHTQLQKNLTERLEEITIKKGKQVLLATHSTELIRAYEPQKILALSNSR
                     GKYLGSDNEKIGLLSGIGTIFSPKIHKLTEDKRLLIVEGVSDERFLKKIFKKLELTWP
                     KNIVTWLWTGKSSERFQLYKQLKNEIPDLKAISMRDRDDDSDHSVGADLKDKSTNHGD
                     SDFLAMKWRRRHIENYFLDISAIARAAETSEEDIRSFFRDRHALAIPDSITSTDIAAA
                     LKDAHGKEIFISGENSLKHNFSITRDEVLEHMNKDEICDDIKYFCQCLINLSQS"
BASE COUNT         2656 a         2748 c         2985 g         2792 t
ORIGIN      
        1 gggtggaatg caagtacccc tacaacatct ttgtcatggt ataatgcata taataaaact
       61 aaacatgata gatcaggagc attccacttt tccactttag aaaatgtaat ggatgcagtt
      121 gctgcttgtg ttgtaatgta ctgtgtaaaa tatggcccgt ttagcttatt ggaagccaat
      181 acgtctctat caacgatcgt taatcaaaac ttttcaattt cattagataa tagcaatcca
      241 gcaagttatt acattcctga aattgaactt ccaccggata caagaacgga tttattaatt
      301 tatgattgtt atcgagaaaa ccataataaa gcatgggtca ctgatcccct agtgctctaa
      361 tttcctgcta aagggagctt ttaattataa acgctccctt cggttgtcgc tatttattat
      421 atatcaatag agttttgaaa ttttaataac tgccatcttg ttaatagtgg ttttattgct
      481 atgttgtgtt gtgtatttat ttttaaacct tcgaatttcg aatatccatt ttccaaggct
      541 tccactgaaa aatgaatggt ttttgttatc gggtttatag cacaaagatt taggtcatat
      601 aaagtatgaa tgtcacttct gagcaataat ccattgctaa tatgattgtg actatcgttt
      661 cgatatacat taatgtgagc agcatcaaga atatcaacaa gcttacagcc cgtaaccgca
      721 caagtaggat ttgattttaa tagctggtcg cgaaatgttt tttgcccact tcgttgtttt
      781 atttgccgtt ctacaatttg ccgttgatct tcacccatta aagtgggggt gtctttatca
      841 gcttcgctgg cgttgagctc cagaataata gtatgttggg cggtaagcaa agcaacagcc
      901 cattgatagt taacttcttg gatggacatt tgcccattgt agcgtggagt ttcatctata
      961 agttgtgaca ttgtaatatt ggataattta cggtactggt tgccgcagta tgctataaat
     1021 tctttagctg gctcaaaaac cactctgggt tcatcaaatt catggccatt atcacaacgc
     1081 cactctgatt ttatagtttt cctttgaaga atttttttgc tttgcagtct aggttaatac
     1141 atttgttacg cttcttatta atgctctgtg tttctaattt ttcaataaca gatataccta
     1201 atatattttc tctgttggta ataatatcac cgtcttttac attttttggt tcgctacaaa
     1261 actgtcatag cgatagaaaa ctgatgagtc atcgggataa ctatcatttc cccagtatct
     1321 aagatcgtct ttctcaattg ccttaaaaaa aaccatactt gctgtaccat gaacttttcc
     1381 cactaatcaa attttggttt gttctatcga acagcaaagc atgttgattt acaagagaaa
     1441 tcatcccaaa gaatatggct caaatcgcaa aattttttgt tctaaccagt catttacttc
     1501 acgcatcctt ttttgcaacg gtaataattc atttcgcaca aacacatttg ccgccttctc
     1561 cacatcccca aaccccccaa cattgctagg cattatcccc atcatttgtg gtggcacacg
     1621 atgcgctgcc atcatgtcat cccgactcac gttcttgatg ttaagaaatt catccttcgc
     1681 tgcgacttct gatagcggga taatctgaag cccgtccttt ttgccgttag gcgagtacat
     1741 aaacaggtta cgaaagttac caggaccttt ggcacttttc atcgcgttgc ggaggttgtt
     1801 cacatcctcc tggttctgcg cggcgtcggt catgtacatg atgaagcctg catggctgcc
     1861 gttaatgtaa tacttccggc ggaacagcgt ggcggactcg ttgagcaggg cggatggaat
     1921 ggcagaaagg taacctggca gaccataaat ctcctggttg atatccggct ccatcaggtg
     1981 gaagacgtta ccttttgtga actgatacgg ctgtgtcgtc aggctgtatt gcacaaacca
     2041 gtaggtatcc aggtctaatc cgcgtcgggt gtattttgcc agagctggct caagggcgat
     2101 aacttcaccg aagcggttcg tgcgtttctc caggtaggcg ttaccaaata ccagatagtc
     2161 ctgcacaaaa cgtgaaaaag cctgctggct gagcagcggg tgagggatgt aggtactggt
     2221 cagaatgttg cactttactg caatcgggga actgtgatgc acagcagcgc ggaaggtgcg
     2281 cgccagtccg tcaaagctga cgggaggctc ataccagcga tctgtctgta cgcattccac
     2341 atagtccagc agttcgcggc ggtcaagtac tggaatggga tcaccgaagc tgaatgcttc
     2401 ggctgtagtc tggcttttat gctggatctg gttcgtcgac gcagcgcggt tcttcttact
     2461 ctttcccatc aaaaaaactc cacaatattg ctggtattgg cggattcgcc ctgcagcggt
     2521 tcgttaaaca gtgcgtgcat cgttgcccag gccagatcgg cgtggctggc ttcttcgctg
     2581 cggctggctt cataggtcgg gcggttgcca ctggcggtgg tggcgcgacg gattgccata
     2641 aatgactggg ctatgtcggt gtgtccggcg tcaaactcca gacggcggtg gctgataatg
     2701 tcgtaggcct tgagtaccag agcgttttta acgttggggt tgtagacaaa ctcccggacg
     2761 gcaggaaaga acgctttcac gttctcataa accccgtgac cgacgcctgt cgagtcgatg
     2821 ccgatatagg tcacgttgta ctgctcggtc agttttttga ttgcatcagc ctgggcgcgg
     2881 aagtccatcc cgcgccactg gtgacgctca agaatgcgaa acttaccgcc tggcacggct
     2941 ggcggtgcca ccaccacgca cccggcgctg tcgccgttct gcgtaccttt tgccgggtca
     3001 taaccgatcc acacttcgcg ccagccaaat gggcgcagcg ccagtgcatg aaagtcggtc
     3061 cagacttccc agctgtccac catgcacgcc tgcagctcgc tgagcgggaa cacggacgcg
     3121 agatcgtcca cgaactcaca catcagcagg ttctggtatt cgtccgggct gtactccatg
     3181 cgtaactggt cgaggtcgaa caggttacag ccgccgcgca ccgcatcttc cacggtgact
     3241 atctggcggt attgcccgtc tgcgcacagc aggccggggg ccagattgct gtgggacagg
     3301 tcgatgtcca ccttatcggc tttgttgcgc ccacggttga acagcgcacc ggaccagaac
     3361 ggataagcac tgtgtgtcag gctggatggc gtggaaaaat aggtttgtcg ccattttttg
     3421 tgaatagcca taccggaagc cactttgcgc agctcctgga atttcggtat ccagaaatat
     3481 tcatccagat acaggttgcc gtggtaactc tgggccgtgc gggcattggt gccgaggaag
     3541 taaagcgtgg ccccgttagg aagcaccatc ggatcgcctt tcagctccac ctccacttct
     3601 ttggcaaagt cgatgatgta ctgtttaaag acgtgggcct gagccttgct ggcagaaagg
     3661 aaaatctggt tacgcccggt cagcagggcg tcaatcaggg cttcacgggc aaagtaaaag
     3721 gtcgcgccga tctggcgaga cttcagcagg ttgcggatgc gattggtttt tgcggcttcc
     3781 caccagtggc gctggtagtt gaacatggag gaatggaaga tttcttccag tttctcaatc
     3841 tgttcatcgg tgaaaacatt cttttccggc tgacggcgcg ggcccttgtt gcggttggcg
     3901 acgttagggt ttaagtcagc ttcgttgccg ccattgttaa acttgccgat ccgcgcgtgg
     3961 cgctccgact ggcgcgccag caggtcaatt tctttgaaat ctttcccttc tttatgctcc
     4021 ttcatgatga gctggcagta gcgtgcggcg gtggtgagct gcatctgatc cagcggccca
     4081 tagtcacccc acttgtcgcg ttttttccag ctgtgaacgg ttgcaacttt ctcgcccagc
     4141 atttcagcaa tgcgggctac gcggtatccc tgaaagtaca gcagcatggc ctgccgacgg
     4201 ggatcgagat ctgcgggtgt cagtgtggtg ttcatggcac aaacctacag ccttgaatga
     4261 cggctttccc cgcctgcggt ttgtgtggtt gtcggtacaa ataccgcgca ttgtttcact
     4321 gcccccatca ccgcaaccat aaggctccag taagtttttt ctaacggagc acggctcatg
     4381 acagtgaaag caaagcgttt tcgcatcggg gtggaaggtg ccaccaccga cggacgcgaa
     4441 atccagcgtg aatggctgga acagatggca gccagctaca acccggcggt gtataccgcg
     4501 ctgattaacc ttgagcacat caagtcttat ctgccggaca gcacctttaa ccgctacggc
     4561 aaggtgacgg cgctgtttgc tgaagaaatc acggaaggtc cgctggcagg caagatggcg
     4621 ctgtatgccg acgttgagcc aacggagtcc ctggtggagc tggtgaaaaa aggccagaaa
     4681 ttattcacct ctatggaagt cagcccgaag ttcgctgata cgggcaaagc ctacctggtc
     4741 ggcctggctg ccactgatga ccctgccagt ctgggcactg aaatgctgac attcagcgcc
     4801 agtgcagccc ataacccact ggcaaaccgc aagcagaatc ctgccaatct ctttaccgct
     4861 gcagaggaaa cggtgatcga actggaagaa atccaggacg acaaaccgtc cctgtttgcc
     4921 cgtgtcacgg cgctgtttac caaaaaagag cagtccgatg acgcccggtt ctctgatgtg
     4981 cataaggccg tggagctggt cgccactgag cagcagaacc tgagcgcacg caccgaaaaa
     5041 tccctgtctg agcaggaaga acgcctgtct gagctggaga ctgccctgca ggcacagcaa
     5101 accgccttta acgaactggt ggacaagctg agtcatgaag acagccgcca ggactaccgc
     5161 cagcgtgcaa caggcggtaa cgcccccgct gacactctga ccaattgctg atggagcaca
     5221 aaacccgatg aagaagaata cccgctttgc ttttaacgct tacctgcagc agctggcgcg
     5281 tctgaacggt gtggcagttg aagaactgtc cagcaagttc accgtggagc cgtctgtaca
     5341 gcagacgctg gaagaccaga tccagcagtc cgccgctttc ctgacgctga ttaacgtcac
     5401 gccagtgact gagcagtccg gtcagctgct ggggttgggt gttggcagca ccattgccgg
     5461 aaccactgac accaccgcga aagagcgtga acctgtcgat cctacgctga tggtcgatgt
     5521 ggaatacaaa tgcgagcaga ccaactttga cacggtactg acctacgcga agctggacct
     5581 gtgggcgaag tttcaggatt tccaggtgcg tatccgtgac gccatcgtga aacgtcaggc
     5641 actggaccgc atcatgatcg gctttaacgg cgtgaagcgt gcgaaaacct ccaaccgtag
     5701 cgaaaacccg ctgctgcagg atgtgaacaa aggctggtta cagaaaatcc gtgaggatgc
     5761 accggatcac gtcatgggca gcagcaccac gggcggtgaa accacaccgc gtgcggtgaa
     5821 agtcggtaaa ggtggcgaat atgccaacct ggacgccgtg gtgatggatg ccgtcaatga
     5881 gcttatcgac gttgtctacc aggacgatga cgatctggtg gtgatttgcg ggcgtgaact
     5941 gctgtctgac aagtatttcc cgctggtcaa caaagagcag gaaaacagtg aaaaactggc
     6001 tgccgatatg atcatcagtc agaaacgcat gggtggcctg caggcggtgc gtgcgccgtt
     6061 cttcccgccg aatgcgctgc tgatcacccg tctggataac ctgtccatct actggcagga
     6121 agatacccgc cgccgttcag ttatcgacac cccgaaacgt gaccggattg aaaattttga
     6181 atccgttaac gaagcctatg tggttgagga ctatcgctgc gctgcactgg tggaaaacat
     6241 ccagattggt gacttcagcg ccgccgcagc agaaaccgga gcgtaattca tgagcctgag
     6301 tcccgcacgg cagcatcgcc tgcgcgttca ggctgaacag gccgctcgcg agggtggtag
     6361 tgttcgccac gcgtcgggct atgacctgat gctgctgcaa ctggcggaag accgccgtcg
     6421 tctcaagggc gttcagtcca cggtcaaaaa agcggaaatc aaggtggagc tgctgccgaa
     6481 atatgccgcc tgggcggagg gcgttctggc tgccggaggc gctcaacagg atgacgtgct
     6541 gatgtacgtg atgctgtggc gcattgatgc cggagattat gccggggcgc tggagatcgg
     6601 gcgtcatgcc ctgcgtcatg gctgggtgat gccgctgggt aaccgcaacg tgcagaccgt
     6661 gctggcagag gaaatggcag atgcagccca gagcgcaatg cttgccgcca cctgctttga
     6721 tgccgacctg ttgctgcaga cgctggagct gacagacggt ctggatatgc cggaccagtc
     6781 acgggcgcgt ctgcataaag cgattggcgc tgtcctgagt gaaagcaacc cggcttccgc
     6841 ccttaatcat ctcaaccatg cgttacagct cgatcctcgc tgtggcgtga aaaaagacaa
     6901 acagcagctg gagcgcagac tgcgcaatga cagccgctga cagaacgtgc ccccgcgcac
     6961 gggcggcacg gggtggcgaa aggcactgcc acatcaaaac cccgtccacc gccctctatt
     7021 tcaggagaaa gcagcatgaa gtttgttgcg ccagaacagg caccggaaca ggcggaaatc
     7081 atcaggaata cgccgttctg gcctgatgtg gacctgtcgg agtttcgcag cgtgatgcgc
     7141 actgacggca cggtgacgca gccgcgttta aagcaggttg cgctgtcggc aatttcggag
     7201 gtcaacgcag agctgtatga gtttcgcaga cgtcagcaga tgctggggta tgtgtcactg
     7261 gctgaggttc cggcggaaca gctggacggc aaaagtgagc gcattcagca ctatttcaac
     7321 gcggtttact gctgggcacg cgccatgctc aacgaacgat accaggacta tgacgccacg
     7381 gcatccggtg tgaagcgagg cgaggaactg gcggaagcca gcggtgattt gtggcgtgac
     7441 gcccgctggg ccatcagccg ggtgcaggat gcgccgcact gcacagtgga gcttatctga
     7501 tgaaagtgcg tgcgcatcag tatgacacgg tggacgcgct ttgctggcgt cattacgggc
     7561 gcacgcaggg tgtcacggag caggtactga aggcaaatcc ggggcttgcc gaatttggcc
     7621 cctttttacc tcacgggctg caggtggagc tgccggacat tccgaccacc accaccgtgc
     7681 agaccgtcca gctatgggac tgaattatga cgcttgagcg aatcagcgcc tttatcacgt
     7741 attgcatcgc cgttgtgctg gcctggctgg gcgatttgtc catcaaggat gcctcaacgc
     7801 tgggcggcct gatgatcggt gtgctgatgc tggctatcaa ctggtactac aaacacaaag
     7861 cctaccagct tctgcgcgac gggcagatct cgcgggagga ctatgaatcc atcaatcgtt
     7921 aaacgctgcc ttgtcggggc cgtgctggct attgctgcca cgctgccggg gtttcagcaa
     7981 cttcacacct ccgtggaggg gctgaaactg attgccgatt acgaaggctg tcgtctgcag
     8041 ccgtatcagt gcagcgcggg tgtctggacc gacggcattg gtaatacgtc gggcgtcatt
     8101 cccggcaaaa ctattacgga acgacaggca gcagaagggc tgatctccaa cgtgctacgt
     8161 gtggagcagg cgctggaaag gtgtgtgaag caacagccgc cgcagaaggt gtatgacgct
     8221 acggtgtcgt ttgccttcaa cgtggggacg ggcaatgcct gtagttccac gctggtgaaa
     8281 ttgctcaatc agcggcgctg ggcggatgcg tgccgacagt tgccgcgctg ggtttatgtg
     8341 aaaggtgttt ttaatcaggg gctggataac cgccgtgcgc gggagatggc ctggtgctta
     8401 cagggagcaa actgaaatga aaaagaaagt aatcagcggg ctgtttctga tgttatggat
     8461 ggcgctgttg atcgcagcaa tggtgtatcc gcaggggatt tttccggtac tggcagcgtc
     8521 cggtgtctgg gtagcctgtc tgctgacatg ggcggtaatt ccggtagcac tggctgcgtt
     8581 aattaagaac ggcccgctct ggcaggagtt gagggcatct ttgctaaaga ccattacccg
     8641 aaaagaaaac gtatttatca gctgggtgat gcgattgctg attgtcgtaa gtttcgcctg
     8701 gacggggtgg gctattaccc tggtctttta tctgctgacc gttattgcct tctggatgat
     8761 ccgtaatcag attgcgcaac aggtagcagc atgaaccggt tgctgctggt tgtgctggcg
     8821 ttattactgg cggcgctggg ctggcagacg tggcggctgg ctgatgccag ccagaccatc
     8881 agcacgcagg cagacgagct tcggagcaaa agccaggcac tggcaaagag caacagccag
     8941 cttatcagcc tgtccattct gactgaaacc aataaccggg agcaggcgcg gctctatgcc
     9001 gaagcagaac agaccagtgt actgctgaga caacgacaac accggattga ggaactgaaa
     9061 cgtgagaacg aggatttacg ccgctgggct gatactcctt tgcctgctga cattatccgg
     9121 ctgcgggaac gccccacact caccggaggt gcagcttacc gtcagtggtt gtccgcgagt
     9181 gacgccgtgt cggctggatc aggcaatgcc gcgcactaac ggtgatctga acgcgttgct
     9241 ggatgaaacg gaggctgcct gggcggtctg tgcagacaaa gtggacatga ttattgcgtg
     9301 tcaggagcga aacagtgaac aaaccacaat ccctgcgcca cgccctcaat aaagcggtgc
     9361 cttatgtccg caataacccg gacaaactgc atctgtttgt ggataacggt tcgctagttg
     9421 ctacgggggc cagctccatg tcatgggagt accgttacac cctgaacgtg gtgattgagg
     9481 atttcagcgg cgaccagaat ctgttgatgg ccccggtttt gctgtggctg cgagataacc
     9541 agcccgatgc aatcaataac ccggtgttac gggaaaagtt attcaccttt gaagtggata
     9601 ttttgcgcaa cgatgtctgt gatatcagcc ttaacctgca actgacggaa cgtgtgctgg
     9661 tcagcactga cggcactgtg tccagcgttg aagctgtagc ggaacccgat gaacctgaag
     9721 aagtgtggac ggtgaaacgt ggctgaactg cagaaggtgg acgactggct gagtgctttg
     9781 ctggcgaatc tggaaccagc cgcaagaagc cgcatgatgc gccagctggc gcaggaactt
     9841 cggcggacac agcagcagaa tatcaggatg cagcgcaacc ctgacggcag cagctatgaa
     9901 ccgcgacggg taacagcacg cagtaaaaaa ggccgtatca aacgtcagat gtttgcaaag
     9961 ctgcgcacca caaaatacct gaaaactgcc gccagtgccg attctgccag cgtgcagttt
    10021 gaaggcaagg tacagcgcat tgcccgtgtt catcactacg gcctgcgtga tcgcgtcagt
    10081 cgaaatggac cagaagtgcg atatgctgag cggcagttac ttggcataga ttctaaaata
    10141 accgccgtta ttcgtaataa gctatttgag tttattaaat aaaaactctc aggattgtga
    10201 taaatttatt aaacattgac aaaaatattt aatgtcgtca catatttcat ctttattcat
    10261 gtgttccaga acttcatctc gagtaattga gaagttatgt tttagtgaat tttcgccact
    10321 aatgaaaatt tcttttccat gtgcgtcttt taaggctgca gctatatctg tagatgttat
    10381 cgaatcaggg atagctaacg catgcctatc tctaaagaag ctacgtatat cttcttcaga
    10441 tgtctctgca gctcttgcga tggctgatat atctagaaag taattttcaa tgtgtctgcg
    10501 acgccatttc atggctaaaa aatcactatc gccgtgatta gttgatttat ctttaagatc
    10561 tgctcctaca gaatggtcgc tatcatcatc acgatcgcgc atgctaatgg cttttaagtc
    10621 agggatttca ttttttaatt gtttgtataa ttgaaacctt tctgaagatt ttccagtcca
    10681 aagccatgtg acaatatttt ttggccatgt tagttctaac tttttaaata ttttttttag
    10741 gaatctttca tcgctaacac cttcaacaat caataatctt ttgtcttcgg taagcttatg
    10801 gattttgggg ctaaagatcg tgccaattcc acttaataga cctatttttt cattatcaga
    10861 accaaggtat ttccctctgg aatttgatag tgccaaaatt ttctgtggtt cataagccct
    10921 gattaactca gtagagtgtg ttgccaatag gacctgcttt ccttttttaa ttgttatctc
    10981 ttctaaacgt tcagttagat ttttctgtaa ttgtgtatgt aggtgagcat ctggttcatc
    11041 tagtagaatg acattaaatt cttctgataa ggccaatgta tatacactta accattgaag
    11101 aaaaccgctt ccttcaacca taatatctct agcattgaag tttttgtgtt ttttaaattg
    11161 ataaccattg acaacgccag c
//
LOCUS       DAMZII010000154        11171 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZII010000154 DAMZII010000000
VERSION     DAMZII010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.154"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            172..387
                     /gene="bdm"
                     /locus_tag="POA77_004065"
     CDS             172..387
                     /gene="bdm"
                     /locus_tag="POA77_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDI6367423.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            489..626
                     /gene="sra"
                     /locus_tag="POA77_004066"
     CDS             489..626
                     /gene="sra"
                     /locus_tag="POA77_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDI6367424.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            783..2480
                     /gene="maeA"
                     /locus_tag="POA77_004067"
     CDS             783..2480
                     /gene="maeA"
                     /locus_tag="POA77_004067"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI6367425.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2614..3624
                     /gene="adhP"
                     /locus_tag="POA77_004068"
     CDS             2614..3624
                     /gene="adhP"
                     /locus_tag="POA77_004068"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI6367426.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3770..4054
                     /locus_tag="POA77_004069"
     CDS             3770..4054
                     /locus_tag="POA77_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDI6367427.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4105..4758)
                     /gene="fdnI"
                     /locus_tag="POA77_004070"
     CDS             complement(4105..4758)
                     /gene="fdnI"
                     /locus_tag="POA77_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDI6367428.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4751..5635)
                     /gene="fdnH"
                     /locus_tag="POA77_004071"
     CDS             complement(4751..5635)
                     /gene="fdnH"
                     /locus_tag="POA77_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDI6367429.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5648..8695)
                     /gene="fdnG"
                     /locus_tag="POA77_004072"
     CDS             complement(5648..8695)
                     /gene="fdnG"
                     /locus_tag="POA77_004072"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8108..8110),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI6367430.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8927..9808
                     /gene="yddG"
                     /locus_tag="POA77_004073"
     CDS             8927..9808
                     /gene="yddG"
                     /locus_tag="POA77_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDI6367431.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10067..10357
                     /locus_tag="POA77_004074"
     CDS             10067..10357
                     /locus_tag="POA77_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6367432.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            10381..11171
                     /locus_tag="POA77_004075"
                     /pseudo
     CDS             10381..11171
                     /locus_tag="POA77_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         2866 a         2832 c         2683 g         2790 t
ORIGIN      
        1 atcgaatagt cacaatcaca atccctatga atatcatagt gttaaaatat aaaaatcatc
       61 aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata
      121 aatctgcgat ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact
      181 tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc
      241 cttcgggcag agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg
      301 gtggaagctt ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg
      361 gtcagtggcc agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg
      421 gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag
      481 gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt
      541 ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtggtaaat aacccaaccg
      601 gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca
      661 ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgggg ccgacgccct
      721 ggcggtaaag caaagacgat aaaagccccc cagggatgga tattcaaaaa agagtgagtg
      781 acatggaacc aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac
      841 tgctggaatt tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact
      901 tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag
      961 catggatcca gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca
     1021 tccaggacac caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga
     1081 tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc
     1141 gccgttcacg cggcgtgttt atctcttacc agaaccgcca caatatggac gatattctgc
     1201 aaaacgtacc aaaccataat atcaaagtga ttgtggtgac tgacggtgaa cgtattctgg
     1261 ggcttggtga ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata
     1321 ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga
     1381 acaaccaaca gttgcttaac gatccgctgt atatgggctg gcgtaatccg cgtatcactg
     1441 acgacgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg
     1501 acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct
     1561 atcgcaatga aatttgttcc tttaacgatg acattcaggg cactgcggcg gtaacagtcg
     1621 gcaccctgat cgcagcaagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct
     1681 tccttggcgc aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc
     1741 gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggtcgat cgctttggct
     1801 tgctgaccga caagatgccg aacctgctgc ctttccagac caaactggtg cagaagcgcg
     1861 aaaacctcag tgactgggat accgacagcg atgtgctgtc actgctggat gtggtgcgca
     1921 atgtaaaacc agatattctg attggcgtct caggacagac cgggctgttt acggaagaga
     1981 tcatccgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tccaacccga
     2041 cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gactgaaggt aacgcgctgg
     2101 tcgccactgg cagtccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc
     2161 agtgtaacaa cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt
     2221 cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gcttgctcag tattcgccgc
     2281 tggtcctgaa cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc
     2341 gcgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacct
     2401 ctgccgaagc cctgcaacag gccattgacg ataatttctg gcaagccgaa taccgcgact
     2461 accgccgtac ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc
     2521 tccctttttc agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct
     2581 gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat
     2641 catcatgttg acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg
     2701 aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt
     2761 gacaaaaccg gcgtaattct gggccatgaa ggcatcggtg tggtggcaga agttggccca
     2821 ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggtttta cgaaggatgc
     2881 ggtcattgcg aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc
     2941 ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta
     3001 aaagtgccag atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggagtcacc
     3061 acctacaaag ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt
     3121 cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgcgaaagtg
     3181 atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta
     3241 gcgattaact cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct
     3301 cacgctgcgg tggtaacagc agtagctaaa gctgcgttta actcggcagt tgatgctgtc
     3361 cgtgcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc
     3421 ccacgtcttg tgctggatgg tattgaagtg gtcggttcgc tggtcggcac gcgccaggat
     3481 ttaactgaag ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt
     3541 ccgttagcgg acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt
     3601 atggtgattg atttccgtca ctaagaggcc tttgctgcga ctgccatgtt cgggtcgcag
     3661 catcgcacac tctccaacat gaaatggcta aatgaattat tgttgcgtaa aaaggaacaa
     3721 taatgatact gttcccagtg aaggaacact actgagagta aagacactga tgaaaatggc
     3781 caatcatccc cgcccggggg acattattca ggaatcactg gacgaactta atgtcagcct
     3841 gcgcgagttt gccagagcaa tggaaattgc tccctcaacg gcaagccgat tgctgaccgg
     3901 aaaagcagct ttgacgccag aaatggcaat taaactttcc gtggtgatcg gcagttcgcc
     3961 gcaaatgtgg ctgaatctgc aaaacgcctg gagtctggca gaggcagaaa aaacggtgga
     4021 tgtgtcgcga ctacgccgtc tggttacgca ataaaaaaat ggcgcttctt aaagcgccat
     4081 tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt tcgcttctgc
     4141 cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac ggctcacctt
     4201 cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca tatggatcag
     4261 gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt agcgaacaac
     4321 ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg tcaccagcag
     4381 cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt tgtacttacc
     4441 gacatccgcc actttatgct cattgccttt caatacttcg acaatgttca acagccacgg
     4501 aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa acatcagtgc
     4561 gacgaaaatc gcaatgccga aaaacgggtg caaaatgcgt cccatctgcg gcgtaccgaa
     4621 ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag
     4681 gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat
     4741 cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa
     4801 taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc gctgccagcg
     4861 gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct ttcggcagac
     4921 cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc gtaccaccga
     4981 ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc
     5041 gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc ccggtcggac
     5101 aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag agcgtgcatt
     5161 tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac ccggcaatgc
     5221 agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta gcgtactgaa
     5281 tgattgcacc agcagacggg cacgccttca ggcagccggg atcttcacag tgcatacagc
     5341 cgtctttacg gatcagccac tccagcttgc cgttctgttc ggtttcgcta aagcgcatca
     5401 ccgtccagga cttggcgctc agatcggcgg ggttatcgta aaccccgacg cagtgcccca
     5461 cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta cagccgatac
     5521 aggtggaaac gtcgataagt tttgcgactt ctgctttgta atcacgcacc tgagaaggcg
     5581 gcgtgatgga gttagttgcg gaccttttga taatgtcctg cgtttccata gccatctgtt
     5641 cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc gtttgcgagt
     5701 ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc gcgacaccct
     5761 caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccattt acattcagtg
     5821 gtttcagacg acgcgttacc acagccaccg cgcggataaa gccacgcttg cttgagacag
     5881 tgacacgatc gccattatta atgcctttcg ccgccgccag cgtttcgctg atttccacaa
     5941 actgttccgg ctgagcaatt gcgttgagca atgcgtgctt ggtccaggtg tggaagtgct
     6001 cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc atccgcagtg
     6061 cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc agcgggttag
     6121 tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc gccattttgt
     6181 tgatggcaaa caggcgtccc atcccttccg gctgcatgat aaacggcccg gttggcgtac
     6241 ccggtgcggc attgccgaag tcaggaatat cgttacccgt ccacttgctg ccgttccact
     6301 ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa gcacggttgt
     6361 acagcacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc cccagaccgg
     6421 acgggtcgga gttatcgcgg ttagccatct ggttgccctg ctctgtccag ctaccggtgt
     6481 agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag ctactcagca
     6541 actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc agcgcatagc
     6601 cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg tagttccagc
     6661 tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacagc tcgcgcaggt
     6721 gatggtagat acccgccaga atttcaccgt cgttacgcgc ttcgcccggc gcatcctgac
     6781 ctttccagtg ccactgcagc cagcgaccgg agttagcaat agaaccatct tcttcagcaa
     6841 agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg acatcgttcg
     6901 actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg ataaccacca
     6961 tgtacttcag cttgctcagg cagctcacca ctttgttttt gtccgggaag gacgcaaccg
     7021 ggttaaagcc ctggcagaaa taaccggtga ctttgccttc gtccatcatg ttgaaatact
     7081 tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag ttgttctctt
     7141 tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc ggatagttgc
     7201 tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc agatacgact
     7261 gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg gtagagagca
     7321 gacctaagtc agtcaggccc tgaatgttgg agtgaccacg caatgcgttc acgccgccac
     7381 cggccatacc catgttaccg agcagtaact ggatcatcgc catagtacgg atgttctgcg
     7441 cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg
     7501 cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc gtaccgcaga
     7561 tgttttcaac gacgtccggc gtgtagcggg aaacgtgctc tttcagcagg ttccacacac
     7621 agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt
     7681 tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac aggccatctt
     7741 cgaaagcaaa atcatcacgc accagcaggc tggcgttggt gtaatgttta acgtattcgg
     7801 cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac gtaatgtccg
     7861 taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta aaacgaggat
     7921 cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag cggaaaccga
     7981 cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg tttttgatat
     8041 ccacccagtg gttggtcatc gcaccgcgac caaatgttgg agcaagactt gctaccgttg
     8101 gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag cgggcaaatt
     8161 tctgtgtcag catcccggtt tcgttgctgg caccggaggc acacagcata ccggtagaaa
     8221 gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg tcacggtcag
     8281 ctttcatcag ctttgcaata cgggagaatg cttcttccca gctaatgcgc tgccatttgt
     8341 cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcgctgttg acgtaatcca
     8401 gcaaaccggc cccttttggg cacagcgcac cacggcttac cggatgatcc gggtcacctt
     8461 caatgtgata aatcgcttct ctggcgtttt tcgcgccatc acccaggcta tacatcaata
     8521 gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg cgtaataatt
     8581 tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg
     8641 ttccagccat accgcccgcg cagattttaa aaaattgtct gcgactgacg tccattgctt
     8701 tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc tgaatctttt
     8761 tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg caaattgacc
     8821 tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa aaaatgctat
     8881 aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga cacgacaaaa
     8941 agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag gattgattcg
     9001 cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt cattaagcgg
     9061 gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga aaggctattt
     9121 actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt ccttagggta
     9181 tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt ggcccagcct
     9241 gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga ttgtaccagg
     9301 attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg ggttacatta
     9361 tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg cgtttattgg
     9421 tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg gatttaacgg
     9481 aattaccgtt tttgtcctgc taacgggagc aagtctgtgg gtttactatt ttctcacgcc
     9541 acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg cggcatttac
     9601 cttaggattt gcttatgctg catggaatgt cggtatactg catggcaatg tcaccataat
     9661 ggcggtaggc tcgtatttta cgcccgtact ttcctcagcg cttgcagccg tgctgctcag
     9721 cgccccgctg tcgttctcat tctggcaagg cgcgctaatg gtctgcggcg gttccctgct
     9781 ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc cggcagttaa
     9841 tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt caccttatat
     9901 ctcatttcga cttcgatcac atatcgataa ataatattat ttcatctatt tttctcattg
     9961 tttttaatca ccattctatt ttaaaaacag tatatcattt ataaatgacg ccgtttaaaa
    10021 ttcgtttata aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
    10081 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
    10141 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
    10201 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
    10261 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
    10321 cgatgaagcc aacggctcgc gcggcaagaa tctctgattg agttaagtgc ccatcacaat
    10381 atgatcactg accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac
    10441 aaccatattg cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc
    10501 aataacaatt tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg
    10561 catgtattag ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca
    10621 gtaagaaata taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta
    10681 accactcaac caccagggct gcaaaacttc acatctatca atcttgataa taaccaactc
    10741 acacattttg atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct
    10801 cttgagtcaa taaattttcc tcaaggcaga aatgtaagta ttacacatat atctatgaat
    10861 aataatgctc tcagaaatat tgatatagat aggctttcat cagttactta atttagtgcg
    10921 gcacataatc aactagagtt tgtgcaatta gaatcttgcg aatggctgca atacctgaat
    10981 ctcagccata atcaattaac tgatattgtt gcaggtaata aaaatgaact cttactgctg
    11041 gatctatccc ataataaact aacaagttta cacaatgact tatttcccaa cttgaatacg
    11101 ttacttatta acaacaattt gctttctgaa attaaaatat tctatagcaa cttctgcaat
    11161 gttcagacat t
//
LOCUS       DAMZII010000155        11141 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZII010000155 DAMZII010000000
VERSION     DAMZII010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.155"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..316
                     /locus_tag="POA77_004076"
     CDS             <1..316
                     /locus_tag="POA77_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDI6367433.1"
                     /translation="LGSLSRREISALIGVAPVNRDSGTMRGRRTIFGGRAGVRTALYM
                     AALVATRFNPVIKDFYTRLITAGKVRKVALVACMRKLLTILNAMIRKNEEWSDSYHKF
                     SL"
     gene            288..714
                     /locus_tag="POA77_004077"
                     /pseudo
     CDS             288..714
                     /locus_tag="POA77_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(800..1363)
                     /locus_tag="POA77_004078"
     CDS             complement(800..1363)
                     /locus_tag="POA77_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6367434.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFACTDVPTMIIRLRV"
     gene            complement(1410..2771)
                     /locus_tag="POA77_004079"
     CDS             complement(1410..2771)
                     /locus_tag="POA77_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDI6367435.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPEMY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVDVYITDMKITEI
                     PQK"
     gene            complement(2823..3053)
                     /locus_tag="POA77_004080"
     CDS             complement(2823..3053)
                     /locus_tag="POA77_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367436.1"
                     /translation="MTVSIVSPSAAAVKPRRHTRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            3731..4036
                     /locus_tag="POA77_004081"
     CDS             3731..4036
                     /locus_tag="POA77_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDI6367437.1"
                     /translation="MEARQGGDSSAGSVHDSPPRLGDARESHKQERNGKKRKKNSNHR
                     NITSGKNIPDTSQTGRVTGDAPAWPASLPAGVSLPGVFARADRRPPPPALYHLFLLP"
     gene            complement(4015..4206)
                     /locus_tag="POA77_004082"
     CDS             complement(4015..4206)
                     /locus_tag="POA77_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367438.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(4203..4625)
                     /locus_tag="POA77_004083"
     CDS             complement(4203..4625)
                     /locus_tag="POA77_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDI6367439.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLSD
                     NELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGENMS"
     gene            complement(4672..4974)
                     /locus_tag="POA77_004084"
     CDS             complement(4672..4974)
                     /locus_tag="POA77_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6367440.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5514..6284)
                     /locus_tag="POA77_004085"
     CDS             complement(5514..6284)
                     /locus_tag="POA77_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367441.1"
                     /translation="MNETLNALICRHARSLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     SYAGEWLTEDEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARVEIRSMADALAKIRK"
     gene            complement(6329..6763)
                     /locus_tag="POA77_004086"
                     /pseudo
     CDS             complement(6329..6763)
                     /locus_tag="POA77_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(6777..6998)
                     /locus_tag="POA77_004087"
     CDS             complement(6777..6998)
                     /locus_tag="POA77_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367442.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(6999..7682)
                     /locus_tag="POA77_004088"
     CDS             complement(6999..7682)
                     /locus_tag="POA77_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDI6367443.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(7759..8052)
                     /locus_tag="POA77_004089"
     CDS             complement(7759..8052)
                     /locus_tag="POA77_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367444.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRWLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            8200..9162
                     /gene="parM/stbA"
                     /locus_tag="POA77_004090"
     CDS             8200..9162
                     /gene="parM/stbA"
                     /locus_tag="POA77_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDI6367445.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEALQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            9165..9515
                     /locus_tag="POA77_004091"
     CDS             9165..9515
                     /locus_tag="POA77_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDI6367446.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            complement(9666..10178)
                     /locus_tag="POA77_004092"
     CDS             complement(9666..10178)
                     /locus_tag="POA77_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367447.1"
                     /translation="MNISVEDLFPESFQRIKEEEEANRPDPNWFAHLPPEKINAYMSK
                     YPFKSFDDIPLDNSGLQYPSLQELEFYFPPAALAANCHQLPERMRQKPVVTMINGLTL
                     LRSLGVQAFNIDPRRWHKIKTYLSQGTIEYPQMSEDGKVIIDGRHRILLIMQIYKVTD
                     VPVFFLKGIS"
     gene            10619..>11141
                     /locus_tag="POA77_004093"
     CDS             10619..>11141
                     /locus_tag="POA77_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-B"
                     /protein_id="HDI6367448.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDL"
BASE COUNT         2692 a         3033 c         2875 g         2541 t
ORIGIN      
        1 actgggaagt ctttcacgtc gagagattag tgcacttata ggtgtggctc ctgtaaacag
       61 agactccgga acaatgcgtg gtagacgaac tatttttggc gggagagctg gcgtaagaac
      121 tgcgctttat atggctgcgc ttgtcgcgac cagatttaat cccgtaataa aagattttta
      181 tacgcgactc attacagctg gaaaagtgag gaaagttgct cttgttgcct gcatgcgcaa
      241 gctgttaacc attctgaatg caatgatccg aaagaatgaa gaatggagtg attcatacca
      301 caagttttca ttataaatat catgctcaag acagttgctt acatcccggc ggccttttgc
      361 cgggatgcca ggagcgcagg ccacggcgcg acaagggtgg cgcgaccgga acggtcgctg
      421 caagcccggg gtttcgcgtc agggatggaa gcccgccagg gcggagacag ctccgctggc
      481 tccgttcacg acagcccgcc ccgccagggg gacgcccgga aaaggcataa gcaggagaac
      541 aacaaaacag acagtaatca gagcagcaga aaaaagaaga acagtagcag tagcagtagc
      601 agtagcagta gcagtagcag gaacaacaac atcatcagta acaggcaaat gacaggtctg
      661 aaaagacaga agcccccatc agaacgtccg cgtaatggaa ggggcggcgc gtgagcgcgg
      721 cccctttcag cgccaccggc gcgcggtctt ttcccggcgc gggcagacag gcgtttatcc
      781 tcccgcctgc ccggtgtttt cagacacgta aacgaataat catcgtcggg acatcggtac
      841 aggcaaacgt gccacgcggc agctcctcgc ggacgtcaga aaacggtaac agcttctcct
      901 gctggcgtgg cccgttgaga cagactgcga ccagcacgcc acccggacgc aacagggaaa
      961 aggcccgcag gatatgccgg atatcctgcc cgtgactgaa cggcggattc atgataatcc
     1021 ggctgtaata ctgcaccggc tgccattcca tgaagtcgcc acactgtacc ctgacaccat
     1081 taaaattttc ccgcagatac cggaccagcc cgctgttaat ctccaccgca tcacacatgg
     1141 cccccggcgc ggtatcccga atcgcccgca gtatggctcc ggtgccggca gagggttcca
     1201 gaatgtgatc acggttgctg atttccgcca gcgtcaccag gcgatcacag accggggcag
     1261 gtgtcacaaa aagctgggat tcagcccgga tttcagcaac cggcgcggcg ttatccagcc
     1321 ttgcgcgcat cagccggata cgttctctta actgactgtg catgtcatta tctccgtcat
     1381 cccccgggga tttccccggg ggatatacct tatttctgtg ggatttccgt gattttcatg
     1441 tcggtgatat acacatccac caggcggaaa ttattgtcca ttgtgcggcg gtagcggtat
     1501 gccccgtggt cttcagcttc tgccacactg cgcacagcct tacagtcccg gggcagggcg
     1561 gcccactgtg cctttgtcat ttcccggaaa ccttcccccg gatagttgac taccggcgga
     1621 cgcttcgcgg cctgactggc gacggcagcc tcttctgccg atggtgcttt gtagtccgtg
     1681 atgcggtcag gcgtcacttt catcgtgccg ctgtacccga gaaaactgta atttggcgtt
     1741 gtgactgaac tcaccgcccc gttgcttttg ttcacacgga tgatggtcag ccactcgccc
     1801 cggctgaaaa cctgtccgcc cggctcaaaa tcctgcgtcc gggtaaccac gccgccgctt
     1861 tcgtccagca tggcacgctc atagatcagg cggttctgat agtggttaac ccagcgttgc
     1921 tgatgctgaa tctgccgttc atgacagcgg atcgcaattt cgcgggcctg ctccgtggtg
     1981 atgatgtcat catccagggc cgaccataac gaccggctcc cttcatactg gcttttttct
     2041 gccgggcgcg gatacgtatc cagcgggaaa caggcgctga tatggtcatg actgctgatg
     2101 agttttgcca tattcagatc cagcgattca gcccgccaca tcgtcagata tttctgcgac
     2161 tgcgcgatgg tcttttcagc cttgcgcaaa tcagcttcga tttttttgat acgacgccag
     2221 cgaacgtccg gacgttcttt atacttcgcg tgaagcagtg ccgaccgcgc ccgttcttcc
     2281 cagtattccg cacgttcaaa gagcatcacg gcacgtttca tgccgttttc aatacgctgc
     2341 gcatcacgac gggcgcggcg ttcgctgtga tgccccacaa gaatgggctg accgggcggg
     2401 atcatcgcgg ccagtctttc cacttcatca agtgcctgtg cggattcact ggcccgcttt
     2461 ccgctgtatc cggtaaaccg ctccgcccgt gcttcctgac gttcagcgag cgtgctgtct
     2521 tcatcctcaa tctctccggc aagtgagagg agcacgtctt cccggcccgg tgtccaggct
     2581 ggcgcgacaa acagcgcctg ttttggtgcc cagcggaaac cggcatcatg cactttttta
     2641 tacatctccg ggtcgaggcg tgacgcagca tacaggcgaa gtttgttatc atccggtgag
     2701 taggtcgcgc ggtacatctg caccgggctg gcggcttccg gggcggtcat ggtgattgct
     2761 gataatgtca taaaatgttc tccttccgtc tgttgccgcc cctcgcgggg cggctcacct
     2821 catcagttaa aagcgcgttt cagttcgagt gtgcggagca cctgcccgaa ggccgtgttt
     2881 ttcatggcgg ggatgtgtac accacgcccc cggcgaaaac tccgggcaat gtgtcgcccg
     2941 aaggctttca gcacagggtc aatttccggc gcggggatgt cttcgcgtct gaaacgggta
     3001 tgacgacggg gctttacagc cgctgcggaa ggtgaaacaa tacttactgt cataatttag
     3061 cctcttatga cgttaattaa tgaaaccgta aagactgttc gcgcagtctc tgcggtatgg
     3121 attaagcagg tccctgacct gcttttttca tatctgcatc tgacgctttc cggtgttttc
     3181 ctccttctgt tcctgcgttc tcattacctg ccagccggat ggctgacaga acacataaat
     3241 gcgtattgcc ggagcctgaa ataaatcctg atttttaaag aacatcaccc gttgagtttc
     3301 gggagccgcg gttttgacgt ttcgcgggaa cctgctgctg tattccctgt ggtatgaact
     3361 tcacccgttt gagaaccttc gccgcaaggg gaatcactgc cacggcctga cgcacctgtc
     3421 ctgggcagaa cgcaaaattt tttaccgggt tttgggtaaa aaattttgtg ggctgttcat
     3481 ttaccctgga cagtcagtgc ggcaggttgt ggctgtctat gcccccggcg acagggactc
     3541 atacgggtgt gtggcacccg gcaggcaatg agcagggacc cgctgcaacg acgtgaagcg
     3601 cggtttgcga aacggcgttg ctccttacat cccggcgtct ttttgccggg atgccaggag
     3661 cgcaggccac ggcgcgacaa gggcggcgcg accgttccgg tcgctgcaag ccgggggttt
     3721 tgcgtcaggg atggaagccc gccagggcgg agacagctcc gctggctccg ttcacgacag
     3781 cccgccccgc ctgggggacg cccgggaaag ccataaacag gagaggaacg gaaagaagag
     3841 gaagaagaac agtaaccaca gaaacataac atcaggaaaa aacattccgg acacatcaca
     3901 aacgggaagg gtaacgggcg acgcgccagc gtggcccgca tcccttccgg caggcgtcag
     3961 cctgccaggg gttttcgcgc gggctgacag gcggcctcca ccgcctgccc tgtatcacct
     4021 gtttctgctg ccctgaagga tatagtgacc accgaggaaa accgcctgct gattcaccac
     4081 aaaatcgcag atttcatcaa caatctgcgc agtggtgagc ggtttctgcc ccatttcacg
     4141 acgccgcgca ttaatcacgg ctttgtaatt gcgcagaatt tcgcgggttt ctgtgctgat
     4201 attcatgaca tgttctcccc gtccagcgcc tgtaacacaa cgtccatcgc ttcgcgttct
     4261 tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata caggcgcttc
     4321 atttcactgc ctgccagcgc catcactttt tccagcatca ccgccgggac tgtcacctga
     4381 cggctggcgc gcttttcttc catcagttca cgaacaacgt cgtgaacaac gctgacatcc
     4441 gcaccatgct caaaggcatc gtccagccgc tgcatgacgg tttccagctc attgtctgac
     4501 aggctccagt cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg
     4561 cattcactgt ccggcacgtc tgtatccagc acttcgcgga taatttcttt aacagtacag
     4621 tacatgttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag
     4681 agccgcgtaa atcgcgttgc attcagggtg aaattcgatg tgacgccaca actgtgcatc
     4741 gcgcatccgg taaagctggg tcagtcctgc atcaccgctg tcgtggtaca gccacaactg
     4801 gcggttaatc acaagggaag tcagaatgat gccgcaggca tcagcgctga ccgtgcggtc
     4861 aaaccagttt gcaccgttca ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc
     4921 accgtccggc aggcggatga agtgccagaa gcctccctga tattccggca tcattttttg
     4981 cgccagcgcg tacacatacg cctcggcata cagaaaatcg ttgccaaaca ggtaaggcag
     5041 aaaggagaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat attgcatgat
     5101 gttttcctcc gttgttcaca tattccgcga caccgggcgg tgccgtgcct ccataaaagc
     5161 cgggcgaaca ggcggggatg caggggcaca agagaagccg cagcctgacg cacacggctg
     5221 acgggagttg cagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc
     5281 agacgggaag gagggaccgg gtgaccgcag cggcgaagcg tacccttgca tcaacgccag
     5341 cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggccac ataactgaaa
     5401 aaccacaagg ccatgccctg catggccggg taaagcggag ccggcggcga cttcctttgt
     5461 gctctgtccg gggaccggca ggaaaaaggc ggggatttac cccgccagtg tcatcatttc
     5521 cggatttttg ccagggcatc cgccatgctg cggatttcta cccgggcttc ccggaccacc
     5581 tcccgcagaa caccacggtc aaaccagcgg cggggcggtt catccggtat acgcagcaca
     5641 tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag gtacatttcc
     5701 accgacgaaa aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg
     5761 tcatcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggcg aaagcggcgc
     5821 gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg ccgcccggat acgccgcgca
     5881 tcttctgcca cccggcaact gacactgcat actgcatctc gcaccgcatc aagaactgcc
     5941 ctgatttcgt cttcggtcag ccattctccg gcatacgaga aagacacctg cgttccgtct
     6001 gccggaagta ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt
     6061 ccggtcagtt tctgaatggc ggaggccagg tgcggcggca gcacgccatc gtggcggtta
     6121 acgagtaacg tcgccagccg gggtgcatcc agccgtacgt aaatgctgat ccagcccgga
     6181 tagttcggat ttcgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg
     6241 ctacgggcgt ggcgacaaat cagtgcattc agcgtttcgt tcatgattca tggtctccct
     6301 gaatcgccgc ccgcaggcgg cgataacatc agtcgttttc gcgtgtgttg tttttcagca
     6361 gggcgcgggc ctgattaacc accgcctgtc ggcgggtgac gcattccggg acggcgaggc
     6421 cgttatcacg ggcggcccac tcacgtttcc acagtgcctg ttcggcggtc tgaatcaggg
     6481 acaccagcag ttcagcagtg acggtcacct ggcggttttc tctcacgtta ttgatgatct
     6541 ccatcgccac gccggaagaa ataccggact caatccgctg gtcttacggg atgtcctcca
     6601 gccgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt ccgtcagcga
     6661 gggcctgaat ttcttccggg gaccagataa ccagcatcag cggcgcgtct cccggatact
     6721 ttgctgccag ctcccggcat agcgtttcgc atgttccgta cattctcgtc tccttctcaa
     6781 tctttgaagc aggaatccag ttccagaatt tcggtatacg cctccataaa caggcgattg
     6841 gcgcgataca gcgtctggcg tgtcaggcgt tcaacaagca catcggaatc aaagcgacag
     6901 cgatgctcca tatcgttcag cctggcccga aggtcacaca tggtgttggc cacctccgcc
     6961 acgtcggcgc gaagtttttc gtgtccggca tagttcattt acgcagcctc cggttcaaac
     7021 cagttatcat tcgcggcccc ctgctgcatg gcccgttgca cggcggccag tcgttgctgt
     7081 ccggcacggt gatactgctc aagcagctcg ataccgatat accggcgtcc ggactggagg
     7141 gcggccacac aggttgagcc gctgcctgca aacgggtcca gcacaatcgc gttcggatgt
     7201 gtgaagctct caatcagtgg ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg
     7261 ttgcctgaat atttccagcc cagcacgtcg ggcaatggct tctgaggcag cgccggacgc
     7321 ccttttgcca ggatgtaagc gcattcgtgg cgatagccca catatgcggc cttcgatgtg
     7381 taggttttgg taaataccag gtggccaacc acgctgaatc ccgcattttt ccaggcggcc
     7441 ataaagcgat cgacgcggtt ccagccatag aagctcacca tcagcgcatc ttttttcagc
     7501 acgcggtaca tttcattaca ggccggttgc agccattcat cggttttatc gccggcgatg
     7561 gtacgcccct gacggtcacg gaaaccgacg agatatggcg ggtcggtgag gatgaaatcg
     7621 acagcattgc ccggaaacgt ggccataaca cgcacgcagt caccctggat aaaacgggac
     7681 ataacagcac tccttctctt acagagggca ccgggagggc gcccctgtgg cgcccgttcc
     7741 ccgctgcctg tgggctgttc accgcgtccg gtaacgggtc aggccgaccg tggaataccg
     7801 cagccgtcgt ctttttgacg gcgaggatat gccgcgaaag cggcttgaag ggcgttcatc
     7861 agaaaagggc gacgggctgg cgtacactgt caccaacgcc agccgggagc ggtaaaagcg
     7921 cccgtcccgt gcccggccgg acgcgggaca ctgaccacag gcagcgttcc tgccgcgccg
     7981 cgtcagccag cgcagtaaaa agaggagccc ggcgacatct tttgtcaccc gccaaacaaa
     8041 acccaaaaac aacccacacc aaacccaata aaacaccaaa acaagacaaa caatcattga
     8101 ttgatggtta aaatggggta aacttgacaa ataaacccac ttaaaaccca aaacataccc
     8161 aaatacacac caaaaaaaac accataagga gttttataaa tgttggtatt cattgatgac
     8221 ggttcaacaa acatcaaact acagtggcag gaaagcgacg gaacaattaa acagcacatt
     8281 agcccgaaca gcttcaaacg cgagtgggca gtctcttttg gtgataaaaa ggtctttaac
     8341 tacacactga acggcgaaca gtattcattt gatccaatca gcccggatgc tgtagtcaca
     8401 accaatatcg catggcaata cagcgacgtt aatgtcgttg cggtgcatca cgccttactg
     8461 accagtggtc tgccggtaag cgaagtggat attgtttgca cacttcctct gacagagtat
     8521 tacgacagga ataaccaacc caatacggaa aatattgagc gtaagaaagc aaacttccgg
     8581 aaaaaaatta cattaaatgg tggggataca ttcacaataa aagatgtaaa agtcatgcct
     8641 gaatctatac cagcaggtta tgaagctcta caagaactgg atgagttaga ttctttatta
     8701 attatagacc tcgggggcac cacattagat atttctcagg taatggggaa attatcaggg
     8761 atcagtaaaa tatacggaga ctcatctctt ggtgtatctc tggtcacatc tgcagtaaaa
     8821 gatgcacttt ctcttgcgag aacaaaagga agtagctatc ttgctgacga tataattatt
     8881 cacagaaaag acaacaacta tctgaagcaa cgaattaatg atgagaacaa aatatcaata
     8941 gtcaccgaag caatgaatga agcgcttcgt aaacttgagc aacgtgtatt aaatacgctc
     9001 aatgaatttt ctggttatac acatgttatg gttataggcg gtggcgcaga attaatatgc
     9061 gatgcagtaa aaaaacacac acagattcgt gatgaacgtt ttttcaaaac caataactct
     9121 caatatgatt tagttaacgg tatgtatctc ataggtaatt aataatggac aagcgcagaa
     9181 ccattgcctt caaactaaat ccagatgtaa atcaaacaga taaaattgtt tgtgatacac
     9241 tggacagtat cccgcaaggg gaacgcagcc gccttaaccg ggccgcactg acggcaggtc
     9301 tggccttata cagacaagat cccagggccc ctttcctttt atgtgaactg ctgacgaaag
     9361 aaaccacatt ttcagatatc gtgaatatat tgagatcgct atttccaaaa gagatggctg
     9421 attttaattc ttcaacaatc actcaaccct cttcacaaca ggagcaaaga agtgatgaag
     9481 agacaaaaaa aaacgcgatg aagttaataa actgattaaa tttttattga tatctttacc
     9541 cccataaacg gggtaaaggt atcaacaaac ctatttttta gctatgtaca gattacatct
     9601 gacaagttat ttctacgtaa caaatcacag tctaaaaatc agtatagctt taccctttgc
     9661 actgttcagc ttattccttt cagaaaaaaa acgggaacat cagttacttt ataaatctgc
     9721 ataataagta gtattctatg cctgccatct ataattactt tcccgtcttc ggacatttgt
     9781 ggatattcaa tcgttccttg acttaaatag gttttaattt tgtgccatcg gcgtggatcg
     9841 atattgaaag cctgaacgcc aagcgacctt aacagggtta acccattaat catcgtgacc
     9901 acaggctttt gtctcattct ctcaggaagt tgatggcaat tagcagccag ggccgccggg
     9961 ggaaaataaa actccagttc ctgtagtgaa gggtattgca aaccactgtt gtccagagga
    10021 atatcatcaa aggatttgaa aggatacttg gacatatacg cattgatttt ttcaggcggt
    10081 aaatgtgcaa accagttagg atcaggcctg ttcgcttctt cttcttcttt aattcgttga
    10141 aatgactccg gaaacaaatc ttccacactt atgttcatca caatatcctc ccttgagagc
    10201 attgctcatc gccaccgata aggatttcat tataaaacac gccagtacag aacaactcac
    10261 tggtttcaaa ctcccgtcgt ccgtcctgac cggtacggta actaacaaga cctgtacaca
    10321 tatcacgatg tgtaccatgc cataaagtga cagtatcccc ccaggtgtct catatcatag
    10381 tttcattaaa acatgctgag gccagagaac agttatgcct gtgccgtttt ttgtggtatc
    10441 aggcgagaat agcacgtggt gtgaatgcct gttttttgat cgttttcaca aaaattgaaa
    10501 tccacagtct tgacagagaa aacacagcgg cgtaattttt gatctgtata taaaaccagt
    10561 ggttatatgt acagtattta attttaattg attgttttta aagtcaaaga ggttttctat
    10621 gagtgataat gaaggtagtg taccgacaga aggtattgat tacggggaca caatggttgt
    10681 gtggccgtca acaggacgaa ttccgggcgg tgatgtgaaa cccggaggct catcaggtct
    10741 cgctccatcc atgcctccgg gatgggggga ttacagccca caaggtatcg cacttgtaca
    10801 aagtgttctt tttcctggaa ttattcgccg gattattctt gataaagaac ttgaagaggg
    10861 agactggtcg ggatggtctg tcagtgtgca tagcccctgg ggaaacgaga aagtttccgc
    10921 tgcacgaaca gttcttgaaa atggtttacg tggtggtttg ccagaaccgt ctcgcccggc
    10981 tgctgtttct tttgcccgtc tggagcctgc ttccggaaat gagcaaaaaa ttattcgtct
    11041 tatggttaca cagcaactgg agcaggtaac ggatatccct gccagccagt taccagcagc
    11101 gggtaataat gtaccggtaa aatatcgtct gacggacctt a
//
LOCUS       DAMZII010000156        10963 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMZII010000156 DAMZII010000000
VERSION     DAMZII010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.156"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            2..1144
                     /locus_tag="POA77_004094"
     CDS             2..1144
                     /locus_tag="POA77_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6367449.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1151..1915)
                     /gene="glcC"
                     /locus_tag="POA77_004095"
     CDS             complement(1151..1915)
                     /gene="glcC"
                     /locus_tag="POA77_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDI6367450.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2166..3665
                     /gene="glcD"
                     /locus_tag="POA77_004096"
     CDS             2166..3665
                     /gene="glcD"
                     /locus_tag="POA77_004096"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDI6367451.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3665..4717
                     /gene="glcE"
                     /locus_tag="POA77_004097"
     CDS             3665..4717
                     /gene="glcE"
                     /locus_tag="POA77_004097"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDI6367452.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4728..5951
                     /gene="glcF"
                     /locus_tag="POA77_004098"
     CDS             4728..5951
                     /gene="glcF"
                     /locus_tag="POA77_004098"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDI6367453.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQLVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5956..6360
                     /locus_tag="POA77_004099"
     CDS             5956..6360
                     /locus_tag="POA77_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDI6367454.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6382..8553
                     /gene="glcB"
                     /locus_tag="POA77_004100"
     CDS             6382..8553
                     /gene="glcB"
                     /locus_tag="POA77_004100"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDI6367455.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVEI
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8909..10591
                     /gene="glcA"
                     /locus_tag="POA77_004101"
     CDS             8909..10591
                     /gene="glcA"
                     /locus_tag="POA77_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDI6367456.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2567 a         2739 c         3052 g         2605 t
ORIGIN      
        1 tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa atgaccttaa
       61 ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga tacccccttt
      121 attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc atatcatctg
      181 gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg cgcaaataga
      241 taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga ttgacgccat
      301 tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt tgaagtcaca
      361 aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa gcggattact
      421 gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac cgtggtatgc
      481 cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt ggtggatgca
      541 gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg tgcgcaaaaa
      601 ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga tcctgcgtga
      661 gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg aacatgaatt
      721 cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct atatcgctga
      781 gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg aggcgattgc
      841 cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt
      901 cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag
      961 ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg
     1021 ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa
     1081 catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcagat
     1141 ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc
     1201 cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg cgtgctgcgc
     1261 gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca tgctggcgat
     1321 cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat
     1381 taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct
     1441 gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt
     1501 tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag
     1561 cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt
     1621 cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc gggctggtgt
     1681 cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtc tcaataatcc
     1741 cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt
     1801 cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg tcgataatta
     1861 accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg
     1921 gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac
     1981 gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt cacagagctg
     2041 gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat
     2101 aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc
     2161 tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca
     2221 catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg
     2281 aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg
     2341 ttgttctgcc taagcaaatg gaacaggtga cagcgattct tgctgtctgc catcgcctgc
     2401 gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg
     2461 aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt aaccccgttg
     2521 gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag gccgttgcac
     2581 cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc attggcggca
     2641 atgtggcgga aaatgccggc ggcgtccact gcctgaaata tggtctgacc gtacataacc
     2701 tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga tcggacgcgc
     2761 tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg
     2821 tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag
     2881 ccagctttga ctcggtagaa aaagccggac ttgcagttgg tgacatcatc gccaatggca
     2941 ttatccctgg cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta
     3001 ttcatgccgg ttatcccgtc gacgccgaag caattttgtt atgcgagctg gacggcgtgg
     3061 agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa gcgggcgcga
     3121 ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa
     3181 atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc
     3241 cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt
     3301 tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg
     3361 atgccaacga acccggtgaa tttgcccgcg cggaagagct gggtgggaag atcctcgaac
     3421 tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga gaaaaaatca
     3481 atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg
     3541 cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg
     3601 ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa ctggagcgtt
     3661 tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga atcaggcaat
     3721 tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc
     3781 tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt acgacccgac
     3841 cgagctggtg ataaccgcgc gtgccggaac gccgctggtg gcgattgaag cggcgctgga
     3901 gagcgccggg caaatgcttc cctgcgagcc gccgcattat ggtgaagaag ccacctgggg
     3961 tgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga
     4021 ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt ttggtggcga
     4081 agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg
     4141 tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg cctccctgag
     4201 cctgcgtcgg gaaatcagcc tgcaagaagc catgaacgaa atcgcccagt ggcaactcca
     4261 gccgctacct attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg
     4321 cggcgaagga tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg ttgcgggtca
     4381 gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg
     4441 gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc aactgatcga
     4501 ctggggcggt gcgttacgct ggctgaaatc gacagccgag gacaatcaaa tccatcgcat
     4561 cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc
     4621 gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg
     4681 cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccaat
     4741 taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc ctgcgcgcct
     4801 gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg ggcgatgaac
     4861 tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc aacgaagtca
     4921 cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct
     4981 gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt gtcgagcaga
     5041 aagtgaaacg cccactgccg gagcgaatac tgcgcgaagg attgcgccag ttagtgccgc
     5101 gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg tttttaccgg
     5161 aacaggtcag agcaaaactg cctgctgaaa ctgtgaaagc taaaccgcgt ccgccgctgc
     5221 gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt tcgcccaaca
     5281 ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg tcagctaacg
     5341 aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa gggctggcac
     5401 gggcgcgaaa taatattgat gcctggtggc ccgcgattga agcaggtgcc gaggcaattt
     5461 tgcaaaccgc cagcggttgc ggcgcgttcg tcaaagagta tgggcagatg ctgaaaaacg
     5521 atgcgttata tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat ttagtcgaac
     5581 ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa ctggccttcc
     5641 actgcccgtg taccctgcaa catgcgcaaa agctgaacgg cgaagtggaa aaagtgttac
     5701 ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc ggttcagcgg
     5761 gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac aaaatgaatg
     5821 cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc cagacgcatc
     5881 tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa caagcccttg
     5941 aaaaggaata aaaaaatgaa aactaaagtc attcttagcc agcaaatggc gagtgcaatt
     6001 attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat tgctgttgcc
     6061 gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc gattgcggct
     6121 tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac taagggctat
     6181 gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact gacgtcgctg
     6241 gaaggtggcg taccggttgt tgttgatggg caaattattg gtgccgtggg cgtttctggt
     6301 ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt ggcgaaataa
     6361 gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt acgcattgac
     6421 gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct ggacgctgcg
     6481 gcgttctggc gcaattttga tgaaatcgtt cacgatctgg caccagaaaa tcgtcagttg
     6541 ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag caatccgggg
     6601 ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta cctggtgccg
     6661 caaccggagc gcgtgacggt ggaaatcacg ggcattgaca gcgaaatcac cagtcaggcg
     6721 gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct
     6781 cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg
     6841 atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt tcggcgtttc
     6901 ctcgatgaat ctctaccgct ggaaaatggc agctatcagg atgtggtggc gtttaaggtg
     6961 gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt acgtaccccg
     7021 gcgcagtttg tcggttaccg tggcgatgcc gctgcgctga cctgcatttt gctgaaaaat
     7081 aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa agacgatccg
     7141 gcgcacatca acgatgttat cgtcgaagcg gctatcagta ccattctcga ctgcgaagat
     7201 tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct gctgggcctg
     7261 atgcagggga ctctgcaaga gaaaatggag aaaaacggtc gacaaatcgt gcgtaaactg
     7321 aatgacgatc gtcattacac cgccgccgat ggctctgaaa tttctctgca cggacgctcg
     7381 ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg ggacagcgaa
     7441 ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat cgccctctat
     7501 gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg
     7561 cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga gacaatgctc
     7621 ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg gacctcgctg
     7681 aacttgcgta gctgtatcgc tcaggcgcgc aatcgcgtgg cgttcatcaa taccggtttc
     7741 ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa
     7801 aaccagatga aatcgacgcc gtggatcaaa gcctacgagc gtaataacgt gctttccggt
     7861 ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag gcatgtgggc aatgccggac
     7921 ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc aaacacagcc
     7981 tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccacca aaccaacgta
     8041 cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga accgctgctg
     8101 gacgatctgc tgactattcc ggtggctgaa aacgctaact ggtcggtgga agagatccaa
     8161 caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg ggtggagcag
     8221 gggattggtt gttcaaaagt tccggatatt cacaatgtgg cgctgatgga agaccgcgca
     8281 acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat tctgaccaaa
     8341 gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca aaacgctgac
     8401 gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt taaagctgcc
     8461 agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc gttattacac
     8521 gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaaagcc ccgacaagtt
     8581 ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc
     8641 gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg cctataacaa
     8701 caatatggac atcttaacga tactctgcgc ctgcgtgtgc agaacagaaa cccttacccg
     8761 atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc tgtaaagtat
     8821 aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag tatatcaagc
     8881 atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt atatgccgat
     8941 gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct tcttcgttgc
     9001 actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta tattatctat
     9061 cctgattgca atattcgcct ttaaaatgcc gattgatatg gcttttgctg ctgcgggcta
     9121 tggctttatt tatggattat ggcctattgc gtggattatt gtcgcggcgg tgttcctcta
     9181 taaattaacc gttgccagcg gtcagttcga tattatccgc agctcggtta tctccatcac
     9241 cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt tactggaagg
     9301 agcggctggc tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg gcctgggctt
     9361 caaaccgtta tacgcggcgg ggctgtgtct gattgccaac actgcgccag tggcgtttgg
     9421 tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat
     9481 tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt tctggctggt
     9541 agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg
     9601 gggaagcttt gctgtcactc agttctttac ctctaactat attggtccgg aactgccgga
     9661 tattacttcg gcgctggtga gtatcgtctc actcgcttta ttccttaaag tctggcggcc
     9721 gaaaaatacc gaaacggcaa tcagcatggg gcaatccgca ggtgcgatgg tggtaaataa
     9781 gccatcttct ggcggtcccg tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg
     9841 gtcaccgttt ttaatcttaa cggtgctggt caccatctgg accatgaagc cgtttaaagc
     9901 gttatttgct ccgggcggcg cgttttattc actggtgatt aatttccaga tccctcattt
     9961 gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt
    10021 gtttaaattc gaccctctct cggctggcgg caccgctatt tttattgcgg cgattatctc
    10081 tatcttcatc ctcggcgtgg gcatcaagaa agggattggc gtctttgccg aaacgctaat
    10141 tagtctgaag tggccgatat tgtcgattgg catggtgctg gcgttcgcct tcgtcaccaa
    10201 ctattccggc atgtccacca cgttggcgct ggtactggca ggtacaggcg tgatgttccc
    10261 gtttttctca ccgttcctcg gctggctggg ggtattcctt accggctcgg acacttcttc
    10321 aaacgccctg tttggttcac tgcaatcgac cacggcacag caaatcaacg tctctgacac
    10381 tctgctggtg gcagcaaata ccagcggcgg cgtaactggc aagatgatct ccccgcaatc
    10441 tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga gaatctgaac tattccgcta
    10501 caccgtgaag cacagtctga tttttgccag cgttatcggc gttatcacgt tgttgcaggc
    10561 gtatgtgttt actggaatgt tggtctcgta aatcaataat gccggatgat cgttaatatc
    10621 atccggcttc atttacatta ttctgtctta ttaattgaaa attatttctc gctttattat
    10681 ccatgcggca ataatgtttt gtgttcagat aacctgctaa ataattagga gcgtgagaac
    10741 atctattttt ttatcacgca aaacaagtct gtattaatta tgttcgcttg atgagcaaac
    10801 accgttctat ctgttgcttt tgcaatctta atcgttaaaa ctaccaacga atttatatag
    10861 ttgcgttgtg aaatgttgtt aattttttgt ttattgaaat ttcgtaaaaa tagccagcca
    10921 gacatcaaat aggggttatt cttataaatt tcacctgttt tct
//
LOCUS       DAMZII010000157        10932 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZII010000157 DAMZII010000000
VERSION     DAMZII010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.157"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            41..951
                     /gene="hlyE"
                     /locus_tag="POA77_004102"
                     /pseudo
     CDS             41..951
                     /gene="hlyE"
                     /locus_tag="POA77_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1158..1604)
                     /locus_tag="POA77_004103"
     CDS             complement(1158..1604)
                     /locus_tag="POA77_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDI6367457.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1696..2355)
                     /locus_tag="POA77_004104"
     CDS             complement(1696..2355)
                     /locus_tag="POA77_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI6367458.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2427..2720)
                     /locus_tag="POA77_004105"
     CDS             complement(2427..2720)
                     /locus_tag="POA77_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDI6367459.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2962..3363
                     /locus_tag="POA77_004106"
     CDS             2962..3363
                     /locus_tag="POA77_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDI6367460.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3483..3851)
                     /locus_tag="POA77_004107"
     CDS             complement(3483..3851)
                     /locus_tag="POA77_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367461.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4371..5066
                     /gene="minC"
                     /locus_tag="POA77_004108"
     CDS             4371..5066
                     /gene="minC"
                     /locus_tag="POA77_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDI6367462.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5090..5902
                     /gene="minD"
                     /locus_tag="POA77_004109"
     CDS             5090..5902
                     /gene="minD"
                     /locus_tag="POA77_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDI6367463.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5906..6172
                     /gene="minE"
                     /locus_tag="POA77_004110"
     CDS             5906..6172
                     /gene="minE"
                     /locus_tag="POA77_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDI6367464.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6544..6888)
                     /locus_tag="POA77_004111"
                     /pseudo
     CDS             complement(6544..6888)
                     /locus_tag="POA77_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6923..7042)
                     /locus_tag="POA77_004112"
     CDS             complement(6923..7042)
                     /locus_tag="POA77_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6367465.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(7003..7188)
                     /locus_tag="POA77_004113"
     CDS             complement(7003..7188)
                     /locus_tag="POA77_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367466.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7289..7462
                     /locus_tag="POA77_004114"
     CDS             7289..7462
                     /locus_tag="POA77_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367467.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7464..7808
                     /locus_tag="POA77_004115"
     CDS             7464..7808
                     /locus_tag="POA77_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDI6367468.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7812..8147
                     /locus_tag="POA77_004116"
     CDS             7812..8147
                     /locus_tag="POA77_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDI6367469.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8204..10525)
                     /locus_tag="POA77_004117"
     CDS             complement(8204..10525)
                     /locus_tag="POA77_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6367470.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYIANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10642..10851)
                     /locus_tag="POA77_004118"
     CDS             complement(10642..10851)
                     /locus_tag="POA77_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367471.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3047 a         2594 c         2392 g         2899 t
ORIGIN      
        1 agtttcatta caatttatat atttaaagag gcgaatgatt atgactgaaa tcgttgcaga
       61 taaaacggta gaagtagtta aaaacgcaat cgaaactgca gatggagcat tagatcttta
      121 taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca taaaagagtt
      181 aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagtcggcg atattaaaac
      241 cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt atgaatggtg
      301 tggtgttgcg acgcaattgc tcgcagcgta tatttttcta tttgatgagt acaatgagaa
      361 gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca tcacgaagct
      421 gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa
      481 actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca gcttttccag
      541 tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg tgtcgtcgcc
      601 ggtccatttg gattaatcat ttcctattct attgctgcgg gcgtagttga agggaaactg
      661 attccagaat tgaagaacaa gttaaaatct gtgcagaatt tctttaccac cctgtctaac
      721 acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac caccgaaata
      781 gccgccatcg gggagataaa aacggaaact gaaacaacca gattctatgt tgattatgat
      841 gatttaatgc tttctttgct aaaagaagcg gcaaaaaaaa tgattaacac ctgtaatgag
      901 tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg ataagcgatt
      961 attctctcca tgtactcaag gtataaggtt tatcacattt aaaaacacag acgtttttta
     1021 aagattgaga gttacctgtt gataataagg tatcgggtaa ctctcattat gacagagcgc
     1081 tttcaaaaac agggctacga aatggcgagc atccagacgt atattaagaa agcccccatc
     1141 aacatctatt aaatttatca ctgcgcccag tcaggtttat ttaagatatg atcctgccag
     1201 tcaatcactt ctgattcttt cactgcgata tgacgcacag agatacgttc accatgcatt
     1261 gccgctttcg aaccagtaag tagcggatgc cacgcaggta aatctttacc ttccgccagc
     1321 aaacgataag cgcaggtcat tggcagccat tcgaatgttg gcagattttc acgggttaat
     1381 ttaatgcagt cgggttcaaa ctcgaaacga cgttcgtagt tccgacattg acaagtttta
     1441 atattgagct ggcgacaggc gacgttagtg aagtagattt cgtcggtgtc ttcatccatc
     1501 agtttatgca ggcaacactg accgcaacca tcacacaacg actcccattc cgcatcgctc
     1561 atttcgtcca gggttttact ttgccagaaa ggtacatcgc tcatcaggtg ttccgccatt
     1621 acgttaaaac gcaccttata accagtctgg cacagcgatg caagttttgc cgccgctttc
     1681 aggcggcaaa aagtattaca aaacgcgagt tgtcaaagaa tgcccatcga aagtgactgt
     1741 cagctcatca ccgctttgca acgggccgac gccatcaggc gtgcctgtca acacaacgtc
     1801 accggccttg agggtaaaaa acttgctcat ataagcgatc aacggaacga ttttatggat
     1861 catgtccgca gtcgtacctt gctggcgttg ttcgccgttt acgctcaggc ccagcgttgt
     1921 attttgtgga tcgccggtaa attccgccgc ggggataaac ccggaaagcg gacaagagtt
     1981 atcaaacgct ttagcctttt cccacggctg cccggctttc ttcatttttc cctgaacatc
     2041 acgcaatgtc agatcaagcg ccacgccgta accggcaatg gctttgcgga catgctcttc
     2101 cgttgcctga cgcagcgtcg cgccaatcaa caccgccagt tcgacttcat gatgaactga
     2161 accgaaatcg gatgggatcg ccaatggctg ccgcagatcg cacagtgccg tttctggttt
     2221 aataaacagc actggctctt cgggcactgc gctgcccatc tctttaatat gtttggcata
     2281 gttactgcca acacagacta ctttactcac cggataatcc agcagcgcac cttgccagtt
     2341 gtgatgttga tacataacgt tcccctcacc ttatcgtgga tatgaaaacc cgcgccgaca
     2401 aagataatgc caccgccgga tatcggttat ttgttagtgt cgtctgtttt ctgccccatg
     2461 acggaaagat gttgcttcag caaatcttcg ggtggtggcg gtaactgcaa atagtaacct
     2521 tgctcggtta atgccagttt aaccttttca atatcagcat tgaccagttt cttccgccca
     2581 tccaacggca gaatcatcgc taactgaggc tgaccaaaac ctttcatcag ttcctcagga
     2641 acacgcgaaa aatcgtcttt tttttcgaca tataaatagg tctggtcacg cttgctgctt
     2701 cgataaatca cacaaaacat acttttactc tttaagatgc caggttttgg cataagctaa
     2761 catcaatgaa acccgcgcca caagccagcc agctgtagat ttctgcggtt tgttgagtcc
     2821 atgccccttt ataagtaatc cccccggcaa aaaatgccgc gccgggattt taccgaacgc
     2881 agaaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt catcgtactc
     2941 attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt attgctgctt
     3001 gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga gttcagcaaa
     3061 ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga tacatatacc
     3121 ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg cgctgatact
     3181 tacctcttcg ggccaggaat cgatgattca gttgatttat ccagatattc accagaactc
     3241 gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg agtgctgcaa
     3301 acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat tcaaattaag
     3361 taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac tcctctctgt
     3421 cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat gtaaacaagg
     3481 catcactcac gacacataaa taacatcttt gtggtgattt gattgatttt tccactccgc
     3541 ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac atcgcaaaat
     3601 acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg taagttttca
     3661 gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc tgagttcaca
     3721 caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc agcgtcatgt
     3781 ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata aaaaatacgc
     3841 atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc atttcatcat
     3901 caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag aagtatcaga
     3961 cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat aacgggtatc
     4021 tgttctttaa tgatgccgca aaaaatggaa agttaagaaa gcctggcgtg gtgaaaaaat
     4081 acacttgcct caatactgga tatttaaaca ggtctggaga tgctgtcacg attagggtgc
     4141 ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt tgattgacaa
     4201 gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt tactctgaat
     4261 aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta tagtcttcgg
     4321 aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg atgtcaaaca
     4381 cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg catgaggcag
     4441 aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc gcatttttaa
     4501 aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac tggtcagcga
     4561 tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc tgcaaagatg
     4621 cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat cctgacggaa ggaaaggaaa
     4681 aagcgccgcg tccagctccc gcaccacagg ctccagcgca aaatacaacg ccggtcacaa
     4741 aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct ccacaatgtg
     4801 atctgattgt tacaagccac gttagcgctg gggccgaatt gattgccgat gggaacattc
     4861 atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc aagtggtgac cgggaaacgc
     4921 aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa tactggctga
     4981 gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta gtcgaaaacg
     5041 ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta tggcacgcat
     5101 tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg cggccatcgc
     5161 cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata tcggcctgcg
     5221 taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt tacgatttcg tcaacgtcat
     5281 tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg aaaatctcta
     5341 tattcttccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag gggtcgccaa
     5401 agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact ccccggcagg
     5461 gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta ttaccaccaa
     5521 cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg cgtcgaaatc
     5581 acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa cgcgctataa
     5641 cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg agatcctgcg
     5701 catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct ctaaccaggg
     5761 tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag ataccgtaga
     5821 acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga aaggcttcct
     5881 caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct cgcggaagaa
     5941 aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac gccgtcgcag
     6001 cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg tcatttgtaa
     6061 atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag atggcgatat
     6121 ttctattctt gagctgaacg tgaccttacc ggaagcagaa gagctgaaat aagcccgctg
     6181 taaaaacgca tttatcttca aggcagagtc atttctgcct tgagtttttc atccctctca
     6241 tccacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct atttccacct
     6301 ccccctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg cctggttgag
     6361 taaataaccc tgttgttagt gtggatatac tgggagatga taaccccgca atttcattat
     6421 tcattgttct cccataggat gaggtttccc gccgtaactg gcgggctttt tttgccccag
     6481 aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt taatcgttga
     6541 tggtcagaac gaccagttca caccagccac cgcgttccac ggggactcca caccggcacc
     6601 atggctatac cccaccccga aatgcccgct taacgtactg ctgaatgagg ctttaatacc
     6661 tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt cactattcac
     6721 tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcaggc agggttcaat
     6781 ttccataccg ttccccagac gcatgttgta actcagcgtt gcacccagtt cacgatatat
     6841 actgcgggta tcgactgatt tcgattccat gccattggat aaatgatagt agcgtctaag
     6901 tgacctaagc tctgctgcaa catcagcccg caggcaccag accaggggat tcatcctgaa
     6961 gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga catcatgatg aaatgatgat
     7021 atcccgcata agaaagaggc attttttaca cgcagtgcgc tgaagtgtag ttggataaaa
     7081 aatcactctt ttcagataat actgtcgtac gcttatcttt cgacaatgag gccgtcggca
     7141 aaataaaatg actaatgtaa tcgttgctga tgaatatctt ctgctcacat aaaaatcaca
     7201 caataatatt gataaatcag attatcttac ctttacaaca ttgcgttaac agtcaatgac
     7261 acttcttgaa taaaaatatt gtcaatcaat gagttattcc tcatttaaga ttattctcat
     7321 tcacgtaaaa aacataattc ctatcatcac agccacgctc attttgaatt atctgaataa
     7381 tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg tgtgtgcaac
     7441 ttacttaata ccggacatct aagttgaaga aaaaaatact tgccttcggg cttatctcag
     7501 cattgttctg ctccacgcca gcgatggcgg atatgaatcg caccacgaaa ggtgcattgc
     7561 taggcgcagg cgttggttta cttactggca atggtgttaa tggcgtactg aaaggtgcag
     7621 ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa aatgcgcgta
     7681 aaggtgcaaa agtaggtgcc gccgttggtg ctgtcactgg cgttctgacc ggcaatggcc
     7741 tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt ctgggtaaaa
     7801 tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggcctgttt gttttcgcac
     7861 caatgacctg ggcgcaggac tacaatatta aaaacggttt gccgtcagaa acctatatta
     7921 cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca gaaattgttg
     7981 cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa tctccggaac
     8041 tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagacccg caaatgctgt
     8101 tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat ttcatatgta
     8161 ccgcgtcgat gaatacgacg cggtttttta gctaacggcg ggatcagaac ttatatttca
     8221 ggcccaccat cactgcggtg tcattgtagc cattatcacc cagctgcacg cccacattcc
     8281 cccagacgct ggccgctgga ttcaggttgc catttacgcc aagtttaatt tcccccaaat
     8341 tactcgcccc atcttgttcg actttcacac cgttcattga gacagcaaaa tctttactgt
     8401 tatggatcca gttcatttca acaaaaggtt caaagtcgtc accgtgcgga ccgctgtttt
     8461 tctcctgagt acgaataaag gtgcggaaac caagtcgggt ctggatgttg ttatttgcgt
     8521 cattctcaat acgtgagcca ttggcttcgg tgtgcgcatt atgatcgacg cccatccaga
     8581 tagcctgtgc ctgtggctgg aatatccacg tgtatttcgc cgcttccaga ccaaaggatt
     8641 tattcagggc aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc gcactatagg
     8701 attcataggc taattcatcc cctttcaccg agtttttaaa ccagctatat tgcgcccagg
     8761 cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa ccaagtggca tacagccctg
     8821 cgcaatagcc tctgacactc cctttcgaac gataatccga cacgctggaa tgagttgagt
     8881 tgtagtcgcg ggcataacca gccatcactc ccaaacgcca actgtcactg tcggtaaaac
     8941 caccggttaa cagctcggcc cctaactgcg agacgtagcg atgcgaggtc gttctcaact
     9001 gtccgttgct gtcacgccag gcattatgcc cgccaatttg acgtagccaa agtcggcttg
     9061 aacgctcctg ttcagttaca ggatcgatgt agcgcatttc acccgcacgg tcgttcagac
     9121 gcataacaaa aagagtgtta gcggctgcca ggttggcgat gtaacttcca gcttcgaggc
     9181 gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc tccggagttt gtttcaccgc
     9241 cgctcgttgc ctggaagtta gtcagatacc agtttttatt ggtcgcttcg gtattacctc
     9301 gggttaatga atattcgtag gcaccggcga aaatcctcga atccttaata aattccccat
     9361 ttgattcccc ctcaacgctg ataatctcta tcccatttaa cgtgtaagct ccgctgcctc
     9421 ctgcattggt aactttaact cgggttgttc cagaggtatt tccattaata acgactttat
     9481 ccgtagctga attatcatca cccaattcag ttctgagatg caatacgcca ttattaccaa
     9541 cgtaattttc agttattgtt aatcttgttg gttcgacatc ccgtccatct gcccgggaaa
     9601 tatagaccgt actattatca accgtgaggt tgctcaccgt tgagtcagct ttaatatccc
     9661 aggtactatt atctgaaagc gacagatagg tatgagtgtt aacatctgtt gaaatattag
     9721 ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa atgaccttta atattattga
     9781 taaaaacatc tgcagccgat gatacctcac tatccgtaat ggtcacgtta ctcgtctcat
     9841 ttttatggta gaggatttca tttgctgacc gtagtgagct accagataaa atatctaccg
     9901 ttgcttcctg accatttatg gaagactcca gcacaattcc agacccctga tgcgcgactg
     9961 cagatgtatt tttcagcgtc accgtgccgc cattataaat tttagcagtc gcggcgtcag
    10021 gacccgttgt ataaatttct ccctggttaa atgaaactgt cccctggcgg gcagcgatgg
    10081 catgagcatt gtccccatgc gtggtgatat caacatcggt taatgtaacg ctggcattgg
    10141 tataatcaag cgtgtcacca gccttgacga cagtgccatt agcgtacgcg gcataacttt
    10201 ctgttccgtg ggttgtgatc tccccttttg ccatatccag ttttgctatg gcattatttt
    10261 cattacttcc tgtatcggta gtactttctt catcaagaga agatacacac gcacgcgcgc
    10321 ttccgggaga tgcacacgca agaacgccga ctgcgtgatt acccgcagta acaataaatg
    10381 tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc agagttgact cttattatgg
    10441 tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc aattgagtaa tcccccgtta
    10501 tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc aaattcactt acgattgcgc
    10561 tagctgttaa tgctgtgctt agaggactat tgtcagaagg atataaaccg cccgttgtta
    10621 caatgacgcc attgttaatg gttaacttgc taccctctcc tgcaacggat acccgtaact
    10681 tccattaccc gttgttataa ctagcgcatt atcaatatct tttacttttc catcttttat
    10741 tgccacagca tgtttatatt catcgtgctg aataaactgt tcagcatatg cattacccac
    10801 cgccagagca ataagtccca aactaaaccc ggagagtttt ttcagtttca tagattaacc
    10861 atccattcat tggattaaca aacattcctg gtcaaataac ctatgcgttc agattaccaa
    10921 aacggagggt aa
//
LOCUS       DAMZII010000158        10861 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZII010000158 DAMZII010000000
VERSION     DAMZII010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.158"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(103..1746)
                     /gene="yeeO"
                     /locus_tag="POA77_004119"
     CDS             complement(103..1746)
                     /gene="yeeO"
                     /locus_tag="POA77_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDI6367472.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1751..1826
                     /locus_tag="POA77_004120"
     tRNA            1751..1826
                     /locus_tag="POA77_004120"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1784..1786,aa:Asn,seq:gtt)
     gene            complement(1864..2814)
                     /gene="cbl"
                     /locus_tag="POA77_004121"
     CDS             complement(1864..2814)
                     /gene="cbl"
                     /locus_tag="POA77_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDI6367473.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2916..3833)
                     /gene="nac"
                     /locus_tag="POA77_004122"
     CDS             complement(2916..3833)
                     /gene="nac"
                     /locus_tag="POA77_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDI6367474.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMALLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4160..4235
                     /locus_tag="POA77_004123"
     tRNA            4160..4235
                     /locus_tag="POA77_004123"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4193..4195,aa:Asn,seq:gtt)
     gene            complement(4291..5226)
                     /gene="ldtA"
                     /locus_tag="POA77_004124"
     CDS             complement(4291..5226)
                     /gene="ldtA"
                     /locus_tag="POA77_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6367475.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5288..6367)
                     /gene="cobT"
                     /locus_tag="POA77_004125"
     CDS             complement(5288..6367)
                     /gene="cobT"
                     /locus_tag="POA77_004125"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDI6367476.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKAGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6379..7122)
                     /gene="cobS"
                     /locus_tag="POA77_004126"
     CDS             complement(6379..7122)
                     /gene="cobS"
                     /locus_tag="POA77_004126"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDI6367477.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7119..7664)
                     /gene="cobU"
                     /locus_tag="POA77_004127"
     CDS             complement(7119..7664)
                     /gene="cobU"
                     /locus_tag="POA77_004127"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDI6367478.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7798..8235)
                     /locus_tag="POA77_004128"
     CDS             complement(7798..8235)
                     /locus_tag="POA77_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDI6367479.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCQTLTRGSLAIPHSHLLS"
     regulatory      complement(8453..8628)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8717..9631)
                     /gene="pocR"
                     /locus_tag="POA77_004129"
     CDS             complement(8717..9631)
                     /gene="pocR"
                     /locus_tag="POA77_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HDI6367480.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSRSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(9834..10643)
                     /gene="pduF"
                     /locus_tag="POA77_004130"
     CDS             complement(9834..10643)
                     /gene="pduF"
                     /locus_tag="POA77_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HDI6367481.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGV
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         3061 a         2887 c         2317 g         2596 t
ORIGIN      
        1 gaatcgtgag aacgaggcga atattagcga tgcccaccca taatgtcaaa gcctgttttt
       61 taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg acacagctgg
      121 ctttttttca cacttttgcg gctcggatcg agggtatttc catagccaac gtccagtaac
      181 cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac
      241 accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa cccggcaacc
      301 ccacatgctc aacatcgaaa cccacatggc gtaacgggca tcacgagcac ctttaaatcc
      361 agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta gccaaatcag
      421 aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca taagcccggc
      481 aaagggagcc gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg
      541 ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat
      601 cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa ttgaaaacgc
      661 gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt gggttaataa
      721 ccgaccactg gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc
      781 ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg
      841 attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt
      901 taaacccaac ccagccccga caaatcccag tcccggccag gagaaaaggc cgtaaatcaa
      961 tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta gcggtatttt
     1021 cgtattccct gcaccacgaa gtgccccgct accaatcaaa gtgatggcag ctgctggata
     1081 actgagtacc gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc
     1141 acccgcgacg aaatcaataa tttgttcgcc aaaatgatga ataagcgttg ccaacagtac
     1201 ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc
     1261 ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag ctgcaaaaaa
     1321 agccataatg accatattga agctgtccgc caatcccacg ccggccatcg catcttttcc
     1381 cagccagctg accagaaaag tgctcaggac acccatcaac aggacacagg cattctccat
     1441 gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt agctcttgcg
     1501 tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc
     1561 cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat cagcaaagct
     1621 gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa
     1681 acagttgggg aatttaaaaa tcgggtttga caaaagattt ttcgccgtta agatgtgcct
     1741 caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     1801 tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt
     1861 ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctgcc gcttgatatc
     1921 ctcaactgac agtcctgcat tacaaagttc cagaaagcgc cagacatagt tacgctgaag
     1981 ttgtcctcgc ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag
     2041 acggattaaa ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc
     2101 aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta atacaatatc
     2161 tgccagcaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg taatcccccg
     2221 tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt
     2281 taagggatga tcgagtggaa caagcaaact atggtgccaa cgaaaccacg ggaaggcgac
     2341 gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg
     2401 caacaacgtc gcaatttcct gtggcgtccc ctggattagc tcgagccgaa cctccgggaa
     2461 aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt
     2521 tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt
     2581 actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat
     2641 gcccagcagt cgcttacccc gtcgaacaaa tatttcgatg ccaagttcat cctccagttc
     2701 ccgaatatga cggctgacgc ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag
     2761 gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa
     2821 ctccgggcag ttcagatttc ccgttattgt taaagtctaa tgcccggcat aacaaataat
     2881 aaaaacccgc atcttattcc atcccgatat aacacttagc tcaccaattg ccactgcctt
     2941 ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc ctgtgcctgt
     3001 ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat ggaaggagtg
     3061 gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc cgattcgggt
     3121 aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat agactcaatt
     3181 tcaccaataa cttttgccgt gagtcgccgt agggaaaaag cctcatcaac acgaagtcta
     3241 atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt cacatcaacg
     3301 ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag caaagcctga
     3361 ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag ttggtgattt
     3421 atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac gatctccgga
     3481 aattcagcgc gaaccgcctg taataaggcc atggtgatgg atgacgcagc ggttcctggt
     3541 gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac cgccagttgg
     3601 gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt tccggcgtct
     3661 gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc accttccagt
     3721 gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc agcctgggtc
     3781 aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt catgttgcct
     3841 ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg caagatgctt
     3901 gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag agattatgat
     3961 agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata aaaggagggt
     4021 atgcaccacg atggttcatt acccaataag attgaaagct caccactttg ttgaaattga
     4081 cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact gccattaata
     4141 tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     4201 gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct gctttctagc
     4261 aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac cagaactaat
     4321 ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta catcaacttc
     4381 ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt ttcggtcaga
     4441 ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt tcgagccatc
     4501 tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc caacaggaac
     4561 attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct gacttacccg
     4621 gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc caatataaat
     4681 cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca aactctcccc
     4741 tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag cttcttgttt
     4801 acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc cgataccaat
     4861 aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca tctcagcgac
     4921 gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg gaatggtgag
     4981 ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca gcatgttact
     5041 taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt gatcaggaac
     5101 agttacagta aacgactgcc ccactaaacg gctaccctct ggaggtaatg gataagttac
     5161 cgccaggcta gtatggctgg caaaaagtag agcaaatgag caaaaaatat ttacacgacg
     5221 catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt tatcagactt
     5281 ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata ttactggcag
     5341 caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc atcgccagag
     5401 ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc agccccaaat
     5461 gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg agatacggtt
     5521 tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga aatccatcca
     5581 gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt ccgaccaaat
     5641 caaatccacc cgcttttgcc aggacatcaa cgccatcctg cggatttggt tgattcaacg
     5701 taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc aggtttgcgc
     5761 caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt gccgctgccg
     5821 gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtaacaccg ttttttgcca
     5881 gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgcctgatgg cgactcattg
     5941 ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata agcccgggga
     6001 taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc gcttgtgctg
     6061 ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg gctgtcactt
     6121 cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac ataaccagta
     6181 ccgctttttt gcccacatgc ggtatgccat tcaaccccgg cattcctgcc agttgtatgg
     6241 caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtca atatgccgtt
     6301 gtgcacgcga catcgcggca ggatcgatgg cagggatcgt attcagtaaa tcggcaagtg
     6361 tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac caagctctat
     6421 agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa gctgaccgag
     6481 gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac ccggcaataa
     6541 tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc catcaatttt
     6601 gccaatgaac acattgccaa gcccttcttc ccgggcataa cgatgacgat acatcaataa
     6661 tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga tcggttcgcc
     6721 acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa tcaatgccag
     6781 accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat cgcggctacg
     6841 tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc ccccggtcat
     6901 cagcgccagc acaagcacgc taaacagtgc cgccagtggt acgccacacc atgcctgcag
     6961 caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag gaaaagtaat
     7021 aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg gtacaggcag
     7081 acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt tactccaata
     7141 cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt tacccgcccg
     7201 gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat tcccacttcg
     7261 ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa cgactgaatc
     7321 tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc tttatcgccg
     7381 ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa tacagcctca
     7441 tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccaacgctc caccgtgcgc
     7501 cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc atcatcaagg
     7561 atttgtgagg tggcgatata cagaacctgt gaagagtccc caataagcgc ctctgcatgg
     7621 cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac tcctgattca
     7681 attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct gaccgcaaaa
     7741 catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct caatccatta
     7801 cgaaagaaga tggctatgtg gaatagccag acttcctcgt gttaacgtct gacaaaatgc
     7861 gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga ttaatgctac
     7921 agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca aaattaccgt
     7981 cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag ctgctatcaa
     8041 tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc caccatcaag
     8101 aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca atatttcagc
     8161 cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta tcgattcggg
     8221 ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg gtctggttga
     8281 taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt cggcactgcg
     8341 atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag gggtataaag
     8401 acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa gttgttggta
     8461 ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc ccaatctcgc
     8521 gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga gggattttca
     8581 ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc tgatttcact
     8641 tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta aaccttccct
     8701 ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact tgctgtcgat
     8761 acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa ctggtttgcg
     8821 aaaaacctaa attacgcgca atgctggcga tgctccagtc actatggcac agtaactcac
     8881 gagcgctggc catccgttgt tggtttaccc acgcattaaa accgattcca tggtatttct
     8941 tgaataactt actgaaataa taaggactta gataaacgtg cgaggcgaca tcttccagac
     9001 gcaaatcgtc tgataaatgc gcatcaatat agcgtaaagc ttttttcatt ttattgtcgt
     9061 ggggattagg cgcacgagtc tggcgcaccg aatctgaccg agatttgttc tctttaataa
     9121 ccacaaaatt gagttgtttt ttgaggcaat tttccacaat caacttcaac aaatcagcag
     9181 aggccataac tcttgagtaa tccatctcag gaactttgcg gaattcatta agtaattcgg
     9241 gatctgcctg ccagcaatca tcgacattca gaatatcgac caaatcgaca tcatcattac
     9301 gcagacgaac ttgcccgcac aatacaaaac cgactaaatg tccggcgatt accagtggaa
     9361 tagagaaatc ggttaatccg gcatgacagc gataaataca aggctgatcg gatttagagg
     9421 cttccaggcc cccacaacga tcgctcatgc gacatcgagt gcttagctga gggtgttgcc
     9481 gcatcatctg acaaaatgga gtgaagttaa acagctcgga aatttcatcg ccgtgaatat
     9541 tgaccacgac aaccgcaagg ctggtcgcct gcgcaaaatc ctgcgcgatt ttattaatga
     9601 gttctgaatt aagtgtcgcc gcagaaatca tgataaaacc tcccagttaa tttattgtta
     9661 tagcaaaaca attgttttac atttaatctt cacatcatcc ctgatgattt actgacgaga
     9721 agcacaaagg acaataacaa aagcctccgt gaagcgggag actttttaat aagttcggaa
     9781 agcagatcac aaacagtaaa aaaataaatg tttataactg ccagagtatt tcgttattcg
     9841 ctaattcctt taggtgtaac actattgaca ggtaaattct ttgcaatgag atagcgataa
     9901 atcgccgcac caacacatgc cccaatgatc ggtgcaataa tgggtacaat gaaataagga
     9961 atattgcgtc caccagtcat cgcaatttct ccccaccccg caagccaggt gaaaatctta
    10021 ggtccaaaat cacgcgctgg attcatggca aaaccggtta atggtccggt agaagcacca
    10081 ataacagcaa ccagaatacc aataagtaaa ggtgcaagcg gtcctttggg tacgccatta
    10141 ccgtcatcgg tcaacgccat aatcaccccc attaacatgg acgttatgac cacttcgacc
    10201 aacgcagctt gccatacatt aatcgccgct gccggataag tactgaagat actgacgagt
    10261 tgtaaacttt ctgcgctacc acgcaccata ttgtgagcag attcaaaatc ggtaaacaga
    10321 ctactgtata gagaatatgc cagaaccgca ccaccaaagg cacccgctaa ttgcgccaca
    10381 caaaatggca gcactttacg ggctgggaaa gaggcaaaca gccacagagc aatggtaaca
    10441 gccgggttga gatgagcgcc ggaaatacct gccgtaaggt aaacagcaag cgaaatccct
    10501 aacccccaaa tgatacaaat ttcccacagt cccagacttg ccccagcaag tttcagggcg
    10561 cacaaacaac caatgccaaa aaagaggaac agtccggtgc ctaaaaactc ggcaacgcat
    10621 tgcgccttga gtgaatcatt catagtgaca ccttctttac ggagtttttc tttatcagcc
    10681 aactatgctc tgattgttgt gtataccgag cgcaactata aaaaattgct ctgcagaaaa
    10741 tattgccaac atgcatgaca gactcgtgta aaaatcgtgc taacataaca aattattgtc
    10801 attgccgaat catgcaacac agcgttaatc agtgttgaaa gtaataatta ctgtcaaact
    10861 t
//
LOCUS       DAMZII010000159        10820 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZII010000159 DAMZII010000000
VERSION     DAMZII010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.159"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..602)
                     /locus_tag="POA77_004131"
     CDS             complement(<1..602)
                     /locus_tag="POA77_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDI6367482.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLK"
     gene            869..2368
                     /gene="ansP"
                     /locus_tag="POA77_004132"
     CDS             869..2368
                     /gene="ansP"
                     /locus_tag="POA77_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDI6367483.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2483..3544)
                     /locus_tag="POA77_004133"
     CDS             complement(2483..3544)
                     /locus_tag="POA77_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDI6367484.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3786..5888
                     /locus_tag="POA77_004134"
     CDS             3786..5888
                     /locus_tag="POA77_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI6367485.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5924..6589)
                     /gene="mcbR"
                     /locus_tag="POA77_004135"
     CDS             complement(5924..6589)
                     /gene="mcbR"
                     /locus_tag="POA77_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDI6367486.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6787..7824)
                     /gene="curA"
                     /locus_tag="POA77_004136"
     CDS             complement(6787..7824)
                     /gene="curA"
                     /locus_tag="POA77_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDI6367487.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8005..8523
                     /gene="mddA"
                     /locus_tag="POA77_004137"
     CDS             8005..8523
                     /gene="mddA"
                     /locus_tag="POA77_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDI6367488.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8520..8969
                     /locus_tag="POA77_004138"
     CDS             8520..8969
                     /locus_tag="POA77_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6367489.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8970..9203)
                     /locus_tag="POA77_004139"
     CDS             complement(8970..9203)
                     /locus_tag="POA77_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDI6367490.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9289..9462)
                     /gene="ortT"
                     /locus_tag="POA77_004140"
     CDS             complement(9289..9462)
                     /gene="ortT"
                     /locus_tag="POA77_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDI6367491.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9657..9752
                     /gene="yncL"
                     /locus_tag="POA77_004141"
     CDS             9657..9752
                     /gene="yncL"
                     /locus_tag="POA77_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDI6367492.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10154..>10820)
                     /locus_tag="POA77_004142"
     CDS             complement(10154..>10820)
                     /locus_tag="POA77_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDI6367493.1"
                     /translation="FLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGN
                     IRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPE
                     NPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDS
                     NVIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVF
                     RGL"
BASE COUNT         2669 a         2617 c         2568 g         2966 t
ORIGIN      
        1 ctttttaata cttcttgtaa tttcggtaac tgacatacag catcagcaat cgcactgata
       61 ttcgtggcat tatcctgaaa ccattcatgc ccaggccccc atgtgcgcat agtgcaaaga
      121 taacagtcca ccagcgttag ttgctcacca aacgcataag gttcagcggt caattgtgaa
      181 ttcagccaga tataaagcga tttacgatat tcaattacat tcttctttaa ctgttcaggt
      241 gcgtcaggag cccaacgctc agggtaatcg gcgaaagtga atgttggata gacattagca
      301 accagccaga ccaatagccg ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca
      361 aggtccgggc gacgatcgag aaccatcaat gcaatcgccg ctgtctccgt cataatctcg
      421 tcattttcca gcgccagtgt cggaatctga cacaacgggt tcagggtttt caataactcg
      481 cgtgaagttc cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc
      541 agtgttagca tcagctcact gattgtagag ccccatccgg gtacgccata caccttaatc
      601 atatgcccct cacaatcttt taaagattaa gtgtaggaca gtacacaggt taaccagcaa
      661 aaggatttgt cattttttcg tcagttgtat taatcccctt caggattgta aggggatgtt
      721 ttgcatcgtt tatcgataac gggtaggatg ctgcgccgat agttttatac ccataaagaa
      781 taatggtgat aactatcatc gccaggatga ataaacattg ttcatggcaa cttatatgac
      841 tttttcatta aagcaatcag ggagagcaat gagtaaacac gacaccgaca cttcagatca
      901 acacgccgcg aaacgccgct ggcttaatgc ccacgaagag gggtatcaca aagcgatggg
      961 caatcgccag gtacagatga tcgccattgg cggcgcgatt ggcaccggct tgtttttagg
     1021 tgcaggagcc cgactgcaaa tggcgggtcc agcactggca ctggtttatt taatttgtgg
     1081 cttgttttcg ttttttattc tgcgtgcatt gggtgagctg gtgctacacc gcccttccag
     1141 tggcagtttt gtctcttatg cccgtgagtt tttgggtgag aaagccgctt atgttgctgg
     1201 ctggatgtac ttcatcaact gggcgatgac ggggattgtt gatattaccg ccgtcgctct
     1261 gtatatgcat tactggggtg cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc
     1321 acttaccatc gttggcacca tgaatatgat cggtgtgaaa tggtttgcgg agatggagtt
     1381 ctggtttgcg cttattaaag tgctcgccat tgtgatcttt ttggtcgtgg gtacagtgtt
     1441 cctcggtagt ggtcagccgc tggatggcaa caccactggc tttcatttaa tcaccgataa
     1501 tggcggcttc ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt
     1561 tgcttttgcc tccattgaaa tggtgggtac agctgccgga gaatgtaaag atccgcagac
     1621 catggtgcct aaagccatta acagtgtgat ttggcgtatt ggcctgtttt acgtcggctc
     1681 cgtggtgttg ctggttatgc tattgccgtg gagcgcgtat caggcggggc aaagtccgtt
     1741 cgtgacgttt ttctctaaac tgggtgtgcc atatatcggc agcattatga acattgtggt
     1801 gctgaccgct gccctctcca gcctgaactc aggtctgtac tgcaccggac gtattctgcg
     1861 ctcaatggcg atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt
     1921 gccgtatgcc gggattctgg cgacactggt tgtgtatgtc gtcggcgtat tcctcaacta
     1981 tctggtgccg tcgcgcgtat ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc
     2041 ttcatgggcg tttatcatcg tgtgccagat gcgcctgcgt aaagcgatta aagaaggcaa
     2101 agcagcggat gtcagtttta aactgcctgg cgcgcccttc acttcctggc tgacattact
     2161 gtttttactg agtgtccttg tgctgatggc gttcgattac ccgaacggga cttatactat
     2221 cgcggcgctg ccgattatcg gtattctgct ggttataggc tggtttggtg tgcgcaaacg
     2281 cgttgctgaa attcacagca ctgcgccagt cgtcgaggaa gatgaagaaa aacaggaaat
     2341 tgtgtttaag cctgaaacgg cgagctaatt gcaaaattgc ccggctacgt gccgggcaaa
     2401 tttagattac tgacgaaggt gtgccgcatg cggcgtgata aaagtcaggc aagtctctcc
     2461 gttttcaccc tcgccacatc tattacagcg caatacgaat cacatcgtcc ggctgggtag
     2521 cttcctgctg tttagtggat ttttgtttca cactgacata cagcgttttg ccatcggcag
     2581 acagcgccag gctgtttgga tgagtcggcg tatcgaacgt tttcaccact ttatagcttt
     2641 tcgcgtcaat cacactgact ttacctgcct gacgatgcgt tacgtaggct tcattacgcg
     2701 ctgggttaaa cagcacagcc agtgattctg gtgccgcaac cttcgccaga atattgccat
     2761 tacgggtatc gaccactaac acttctgcgg ctttagaatc ggtgataaat gcacgctgcc
     2821 tggcggtatc aaggctaatg ttgataaaga agtgctcttt gccgtcatcc agcagctttt
     2881 tacggctgag gattttattg tcggcggtgt cgatggtaat caattcgccg tcagcgttag
     2941 tggtgtaaag acgtttgcct ttgctatcca gcgccagacc ggtactcatt ttaccggtgt
     3001 tctggatggc ggttttcagt ttgatattct cgccatcaac gacccaaatc acgctctctt
     3061 taccaatacc actgatgtaa acggtgttcg tggcatcatc agctaccagc tcacgcggtt
     3121 gcagcgggcg cacctcttcc gtgcgcttac gatcatccag caccagacgg cctttcacct
     3181 cgcccgtttt ggcatctatc gccgtgaccg cgctgtttac ggtgttacca aaccacaacg
     3241 tctgagtcgt gttattgatg gtggcaccaa acggcttgag atcgttatgg atcgcctgcg
     3301 tcacttccag agtgaccgga tcaagacgat aaaccacgcc acctttatcc agtttgcggc
     3361 tttgcgaagt ggcgagccac agtgcgtttt cttgctggct ataagccatt tcgtaggcac
     3421 ctttacctac cgctttacgc agcatttctt ctgcggcctg cgtactgaat gatgaagcaa
     3481 caagcaatga acctaacagt aatgaaccac gcaggcgcga tgaaaacaga tgacgtaaat
     3541 gcatgacgac tccctttgat gaaccgatat ttttgctgct tcacaatgct tcgctaagcg
     3601 taatcatcgt tacgctcttg ttataaatgg gaatggtaat cattattttc tcaaatgtgg
     3661 agcatttctt gccgttacga ctacaatttt ctcaattact taacgtatcg tgccgtttcg
     3721 ttttccataa atttacaatt tcattaccat aacggcgttt tcccattctt aacctggctt
     3781 ttttcatgaa gattttttcc gtccgacaga ccgttttgcc cgcactgctt gtcctttccc
     3841 ccgttgtttt tgccgctgat gaacagacta tgattgtcag tgccgcaccg caggtggttt
     3901 cagaactgga taccccagca gcagtaagcg tggtggatgg cgaggagatg cgcctggcaa
     3961 caccgcgcat taacttgtcc gaatcactga ccggcgtgcc tggtttgcag gtacaaaacc
     4021 ggcagaacta tgcgcaagat ttacagctgt cgattcgcgg atttggctcc cgctccactt
     4081 acggtattcg cggtattcgc ctgtatgtgg acggtattcc cgccaccatg cccgacgggc
     4141 aagggcaaac atccaacatc gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc
     4201 ccttctctgc cctgtatggc aacgcgtctg gtggggtaat gaatgtcacc acccagaccg
     4261 gacaacagcc accaaccatt gaagccagta gttactacgg cagttttggc agctggcgct
     4321 atgggctgaa agcaacgggc gcaacgggag acggcacaca gcctggcgat gtcgattaca
     4381 ccgtctcaac cacgcgtttt acgacccacg gctatcgtga ccatagtggc gcacagaaaa
     4441 atttagctaa tgccaaactg ggcgtacgca ttgatgaagc cagcaaatta agtctgattt
     4501 tcaatagtgt ggatatcaaa gcagatgacc caggtgggct aaccaaagca gaatggaagg
     4561 ctaatccaca acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa
     4621 ctcaggctgg gttgcgctat gagcgtagcc tgagcgcgca agatgatatg agtgtgatga
     4681 tgtatgccgg agagcgagaa acgacccagt accagtcaat cccgatggcc ccccaactta
     4741 acccgtcaca tgcgggcggc gtgattaccc tgcaacgcca ttaccagggg atagacagcc
     4801 gctggacaca ccgtggtgaa ctgggcgttc cggtcacgtt cactaccggc ctgaactacg
     4861 aaaacatgag tgaaaaccgc aagggctaca ataacttccg cctgaatagc ggcatgccgg
     4921 agtacgggca aaaaggtgag ttgcgtcgcg acgaacgcaa tctgatgtgg aacatcgatc
     4981 cctatttaca gacgcagtgg cagctgagcg aaaaactgtc gctggatgct ggcgtgcgct
     5041 acagctccgt gtggtttgat tccaacgacc attacgttac tccgggtaac ggcgatgaca
     5101 gcggtgatgc cagttatcat aaatggctac ctgccggttc gttaaaatat gcaatgaccg
     5161 atgcctggaa tatctatctg gcagccgggc gaggttttga aacgccgacg attaatgagc
     5221 tgtcttatcg tgctgatggg caaagcggta tgaactttgg tttaaaacca tccaccaacg
     5281 atacaattga gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt
     5341 ttcagaccga cactgatgat gaaattgttg tcgatagcag tagcggtggg cgtacgactt
     5401 acaaaaatgc cggaaagacc cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg
     5461 caggagattt tcgcgtaaac gcgtcctgga cctggcttga tgcgacctat cgcagcaatg
     5521 tttgcaatga acaggattgt aacggtaatc ggatgccagg gatcgcccgt aatatgggct
     5581 ttgcgtcgat aggttatgta ccggaagatg gttggtatgc aggcacggaa gcgcgttata
     5641 tgggcgatat tatggcagat gatgaaaata cggcaaaagc gccgtcttat actctcgtcg
     5701 gcttattcac cgggtataaa tacaattacc acaatttaac tgtggattta tttggtcgtg
     5761 tcgataattt attcgataaa gaatacgttg gttctgtcat tgtcaatgag tcaaacgggc
     5821 gatattacga accttcgccc ggacgaaatt atggtgtcgg catgaatatt gcgtggagat
     5881 ttgagtaatt accttgatgc ccggtatttg ccgggcattt actttaacga ttgtattgct
     5941 ggtataaaat agcaatattt tgttgcataa tttcagcaag acaatgtctg catccctctt
     6001 tgtcttttgc ttttaacgca gcgagtaaca gatgatagtt ttctatatgc tcccgtaatt
     6061 ccgctggatt aatcgcttca tagagataat gtaaaccagg ccccatcctg acccacagtt
     6121 gctcaatcat ctcacacagg atgggcatat ttgagcgatg ataaatcgct aagcgaaata
     6181 gcctgtttac attaatgatt tgttccatgt cgcccttttc ttgcgcctgc tgtaatttct
     6241 cgagcaattc ctggagttcc gccaggtctt gcggggtgag gttttcaaca gccagagcaa
     6301 ctgccattaa ttccagttcg tagcggatcc gattgatttc gtctaattga cgtttcctca
     6361 cttccggaac tgtaaatgct tgtgcaggtg cgacagaaag cgcattcacc gaaaccagac
     6421 gtaataatgc ttcacgcaca ggtgtaatac tcatacctaa ttgctccgcc agatttttag
     6481 taatcaggcg tgcgccaggt tttaatgcgc caatactaag ctgatgtttc aggtcgttct
     6541 caacctgcag agtcaaactg acatgtttca tttttcccgt tccaggcatt ttaattcctt
     6601 cgcgtgtttt ctgtccaacc ctgcctgcat ggcagaagga gttcacctgg tgtattaaag
     6661 tgaataatca taaaccactc acctttgttg ttattagcag cagaacaatg acgtggttag
     6721 ttaattaaag ctgaaaataa gctttttgcc tggattttta tggcggctta cgccgccgtt
     6781 gaaactttaa tcatcacccg cgacgcggat caccactttg ccgaagtttt tacccttcag
     6841 caggccgata aacgtctgtg gcgcattctc taaaccatca gtaatatctt cgcggtagtg
     6901 gattttatcc tctttcaccc attgccccat ctccttctga aactcatgga tgcggtgacc
     6961 ataatcctga gcgataataa acccttgcaa gcgaatacgt tttttcagca ccgttgccat
     7021 caacaggggt aaacgatccg gaccgggtgg tagctctgta gcgttatagc tgctcactaa
     7081 gccacaaacg ggaatgcgcg cagatgtatt aagtaatggt agcactgcat caaatacctt
     7141 accgcccacg ttttcataat agatatcaat accttttggg cacgctttca ccagttgttc
     7201 ggcaaaatca tccgcgtggt gatcaagaca aacatcgaag cccagcacct cgatagcatg
     7261 gcggcatttt tccgcgccac cggcaacccc caccaccctg caacctttaa gtttgccgat
     7321 ttgccccacc gttgctccca caggacctgt cgccgcagct accaccaacg tttcgccctc
     7381 tttaggctga ccgatatcca gcagccccat ataagcggta aagcctggca tccccagcac
     7441 acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat catcaccact
     7501 ggatatgtca tagtcctgcc atccactgta gcccagcacc cagtcgccag gctgataatc
     7561 aggatgattc gactccacaa cacggctcac cgtgccgccg accatcacgc cgccaatatc
     7621 aacaggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg ggtccaggga
     7681 caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgacat catcttcttc
     7741 aagacggaaa ttctccggaa ccggtgcgcc atgtggacgc gaggccagaa cccaacgtcg
     7801 attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga atccatcaat
     7861 agtagcaggc tcacgggatt ggttttgttt gcagccgtca catcccctct gcaacacgca
     7921 ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga taacatcatc
     7981 atcattattt tatcgaggcc agacatgtcc atccgttttg cccgcaaagc cgattgtgct
     8041 gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa
     8101 acggttgatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc aggttatccg
     8161 gtgctggtga gtgaagaaga tggcgtagtg acgggatatg cctcgtttgg cgactggcgt
     8221 agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc cgatcatcag
     8281 ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg ggattgcggg
     8341 aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cctcgctgca tctgcaccag
     8401 tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtgg gcactaaatt tgggcgctgg
     8461 ctggatctga catttatgca gttgcaactc gacgaacgca ctgaaccgga cgcgattgga
     8521 tgaatcagtc gctcaccctt gcttttttga ttgccgccgg aattggtctg gtagtccaga
     8581 acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc atgttgctga
     8641 actcactggt ggggattgtg ctctttgttt ctattttatg gtttaaacaa ggcatggcgg
     8701 ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacccggc ttgctgggat
     8761 cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg acaactattg
     8821 cagtactggt cgccagccag ctaattggcg gtctgatgct ggatatcttc cgcagtcatg
     8881 gcgtaccact acgggcgttg ttcggcccaa tttgtggcgc aattttactt gtggttggcg
     8941 cctggttggt agccagacgc tcattttaat cagaggatgg tgccgccttt ggttaactgt
     9001 tcgtggcgcg cttccatatc ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc
     9061 aaacgctgct gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg
     9121 attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg
     9181 atgacttcat ccagatgcga catactttct ccttaaccac ttgtgaaatt tatcgattca
     9241 tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt aaaacagtga
     9301 aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa aggcgcttaa
     9361 gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc
     9421 cataaccgca taaaaaacaa gcatatgttg atagagagac atgtacgggc gtccagccag
     9481 atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt acatcaagtt ttttcaccag
     9541 tttgagaaaa aatcatcaaa cagcaacttg cccttatcca tcttcagaat gagtattatt
     9601 gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg gcaaaaatga
     9661 atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt ttgtttactc
     9721 ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc agcacgtact
     9781 gtcagagaaa tcaactctct gacagtacgc aagatcttct catactctcc aggtttacaa
     9841 cattatcctt aatatttact tctttttgct ggcatctaca ttgaaatgag tagaaaacat
     9901 ccccctactg tcgtctatac aaacagctta ctgattcaaa cggaatcttg ttttcacatt
     9961 tttctatcat gaatacttta tacgcgcggg agtctataaa tgctttatac tttcaaggaa
    10021 taacgtaaat ataaaaagat ggcgtaaagt atatgcatgg caggatgttt gcaacctcct
    10081 tgatgacgct gccccattga aattgcaaac cccgccctgg ttatcttgcc catttatcat
    10141 gcaaaacaga taattatagg cctctgaata cctcagaaag attgaatttt ttatagtccc
    10201 tctcttgaat atttccatct gatatcaatc gattcatctc ttcaacaaaa taatttatat
    10261 catcacactt gttattatcc ggaagatctg agtagttata attccaccat tttattctca
    10321 acagttcatc aataacattt gaatcaaacc taaatctgat tatttttgca ggaactcctg
    10381 caactatggc atatgggggc acatctttgg taacaacgga attggcagca ataacagcac
    10441 catcacctat tgcgatgcca cctttcaata caacatcatt tcctatccag acatcatttc
    10501 ctataatcgg attttcaggt ttggtaatga atggtttcaa tgtccagcta cgttcaaatt
    10561 cactcatagc aaacttatca aattcaccgt tataagtcat catgtgagta gtaaaacgat
    10621 tcaaaggatg atttccaccc attattttta catttgatgc aatactacaa aatcgaccta
    10681 tacgtatatt ccctgaaaaa tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat
    10741 ttttcacagg cattgttgaa tattcttcag caataacatt atcttcaatt gttatcttgt
    10801 caccttttga gaaaagaaat
//
LOCUS       DAMZII010000160        10663 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZII010000160 DAMZII010000000
VERSION     DAMZII010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.160"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(11..175)
                     /locus_tag="POA77_004143"
     CDS             complement(11..175)
                     /locus_tag="POA77_004143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367494.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            284..778
                     /gene="napF"
                     /locus_tag="POA77_004144"
     CDS             284..778
                     /gene="napF"
                     /locus_tag="POA77_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDI6367495.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            768..1031
                     /gene="napD"
                     /locus_tag="POA77_004145"
     CDS             768..1031
                     /gene="napD"
                     /locus_tag="POA77_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDI6367496.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1028..3514
                     /gene="napA"
                     /locus_tag="POA77_004146"
     CDS             1028..3514
                     /gene="napA"
                     /locus_tag="POA77_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDI6367497.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3521..4216
                     /gene="napG"
                     /locus_tag="POA77_004147"
     CDS             3521..4216
                     /gene="napG"
                     /locus_tag="POA77_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDI6367498.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4203..5066
                     /gene="napH"
                     /locus_tag="POA77_004148"
     CDS             4203..5066
                     /gene="napH"
                     /locus_tag="POA77_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDI6367499.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5063..5512
                     /gene="napB"
                     /locus_tag="POA77_004149"
     CDS             5063..5512
                     /gene="napB"
                     /locus_tag="POA77_004149"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDI6367500.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5522..6124
                     /gene="napC"
                     /locus_tag="POA77_004150"
     CDS             5522..6124
                     /gene="napC"
                     /locus_tag="POA77_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDI6367501.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6143..6760
                     /gene="ccmA"
                     /locus_tag="POA77_004151"
     CDS             6143..6760
                     /gene="ccmA"
                     /locus_tag="POA77_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDI6367502.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6757..7419
                     /gene="ccmB"
                     /locus_tag="POA77_004152"
     CDS             6757..7419
                     /gene="ccmB"
                     /locus_tag="POA77_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDI6367503.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7461..8198
                     /gene="ccmC"
                     /locus_tag="POA77_004153"
     CDS             7461..8198
                     /gene="ccmC"
                     /locus_tag="POA77_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDI6367504.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8195..8404
                     /gene="ccmD"
                     /locus_tag="POA77_004154"
     CDS             8195..8404
                     /gene="ccmD"
                     /locus_tag="POA77_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDI6367505.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8401..8880
                     /gene="ccmE"
                     /locus_tag="POA77_004155"
     CDS             8401..8880
                     /gene="ccmE"
                     /locus_tag="POA77_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDI6367506.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8877..>10663
                     /gene="ccmF"
                     /locus_tag="POA77_004156"
     CDS             8877..>10663
                     /gene="ccmF"
                     /locus_tag="POA77_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDI6367507.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEEL"
BASE COUNT         2192 a         2732 c         3192 g         2547 t
ORIGIN      
        1 tcttttagtg ttaaattctg atgagggagc aaaccagcag aaatatctct ggtgaattaa
       61 tcgctatata aatatgttta taaccatttg aaatgtgagc aaaagcccgt ttttgccacg
      121 cttcccgcca ctcttttgat cctgctacag gttttacccc gatcggggta tgcatctttg
      181 acacatcctt taatatctta gcggctataa aaatggctta ttaattatgc ggtttatttg
      241 gtcgctctca attttcagag cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc
      301 ccgtcggggc atactcactg gtcgctggcg caaagccagt aacggtatcc gtccaccctg
      361 gtccggtgat gaatctcatt ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc
      421 ttgtgaaaac aacattctgc aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa
      481 taacgagtgc agcttctgtt acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc
      541 gcgccacacc agggcctggg atttgcagtt caccattggg gacgcgtgcc tggcgtatca
      601 gtcagttgaa tgccgccgct gtcaggatag ctgtgagcca atggcaatta tctttcgccc
      661 gacgctctcc ggaatctacc agccgcaact taatagccaa ctttgcaacg gatgtggagc
      721 atgtgcagcc agctgtccgg tatcagccat aactgcggag tatctccatg cacactaact
      781 ggcaagtttg cagcctggtc gtgcaggcca aaagcgaacg aatttcagac atcagcaccc
      841 aactgaacgc ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga
      901 ttgtggtggt ggaagcagaa gacagcgaaa cgctgatcca aaccattgag tcagtacgca
      961 acgtagaggg cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag caaggtgagg
     1021 aaacaccatg aaactcagtc gtcgtagctt tatgaaagct aacgccgttg cggccgctgc
     1081 ggcggctgcc ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc
     1141 cattaaatgg gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg
     1201 aacgcagcag gggcgtgtgg tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg
     1261 cctgaactgc attaagggct atttcctgcc caagatcatg tacggtaaag accgtttgac
     1321 gcagccgctg ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat
     1381 cacctgggat caggccttcg atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa
     1441 aggaccggaa tcgatcggta tgttcggttc tggtcagtgg actatctggg aaggttatgc
     1501 cgcgtccaag ctgttcaaag cgggcttccg ttcgaacaac atcgacccga acgcacgtca
     1561 ctgtatggcg tcggcagtag ttggctttat gcgtaccttt ggtatggatg agccgatggg
     1621 ctgctatgac gacatcgagc aggctgacgc gtttgtgctg tggggcgcta acatggcgga
     1681 gatgcacccg atcctctggt cacgcatcac taaccgtcgt ctctctaacc agaacgtcac
     1741 cgtggcggtg ctttctacct accagcatcg tagcttcgag ctggcggata acggcatcat
     1801 ctttacgccg caatctgacc tggtgatcct gaactacatc gccaactata tcattcaaaa
     1861 caatgcgata aatcaggact tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga
     1921 catcggctac ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga atccgggttc
     1981 tgacgcctcc gaaccgatga gctttgaaga ttacaaagcc ttcgttgctg agtatacgct
     2041 ggaaaaaacc gctgaaatga ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct
     2101 gtatgccgat ccgaacaaga aagtcatctc ctactggacg atgggcttca accagcatac
     2161 ccgtggtgtg tgggccaaca acctggtcta caacctgcac ctgctgaccg gcaaaatttc
     2221 ccagccgggt tgcggtccgt tctccctgac cgggcagcct tctgcgtgtg gtactgcgcg
     2281 tgaagtgggc acctttgctc accgtctgcc tgcggacatg gtggtgacta acgagaaaca
     2341 tcgtgatatc tgcgagaaga agtggaatat cccgggcggc accattccgg caaaaatcgg
     2401 tctgcacgcg gtggcacaag accgtgcgct gaaagacggc aagctgaatg tttactggac
     2461 catgtgtacc aacaacatgc aggccgggcc gaacatcaat gaagagcgta tgccgggctg
     2521 gcgcgatccg cgcaacttca tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc
     2581 cgccgacttg atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc
     2641 cgaacgccgt actcagttct ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga
     2701 tctctggcag ttagtccagt tctcccgccg cttcaaaact gaagaagtat ggccggaaga
     2761 gctgctggcg aagaaaccgg aactgcgtgg caaaacgctg tacgaagttc tgtatgccac
     2821 ccccgaagtg agcaaattcc cggtatccga actggcggaa gatcagctga acgatgaatc
     2881 ccgcgagctg ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg
     2941 cggtcacggt cacgatctcg cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg
     3001 gccggtggtg aacggtaaag aaacacagtg gcgttacagc gaaggtaacg acccgtacgt
     3061 gaaagcgggc gaaggctata agttctacgg taaaccggat ggcaaagcgg tgatcttcgc
     3121 actgccgttc gaaccggcgg cggaagcacc ggatgaagag tacgacctgt ggctctctac
     3181 cggacgcgtt ctggagcact ggcacaccgg cagtatgact cgccgtgtgc cggaactgca
     3241 ccgcgccttc ccggaagcgg tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct
     3301 gcgccgtggc gacaaagtga aagtggtttc tcgccgtggc gaagtgatct cgattgttga
     3361 aacgcgcggt cgtaaccgtc cgccacaggg cctggtgtac atgccgttct tcgacgccgc
     3421 acagctggtt aacaaactga cgctggatgc gaccgatccg ctctcgaaag agacggactt
     3481 caagaagtgc gcagtcaaac tggagaaggt gtaagccatt atgtcccggt cagcgaaacc
     3541 tcaaaatggt cgccgccgct ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc
     3601 cgtgggtgtg gcgctggggt tacaacagca aaccgcacgc gcatctggcg tgcggttgcg
     3661 cccgcccgga gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg
     3721 tgttcaggct tgcccttacg acaccttaaa actggcgacg ctggcctctg gtctgtcggc
     3781 gggcacgcca tattttgtcg cacgggatat tccttgcgaa atgtgtgagg acattccgtg
     3841 cgccaaagtg tgcccaagcg gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg
     3901 gatggggctg gcggtactgg tggaccagga aaactgtctc aactttcagg ggctgcgctg
     3961 cgatgtttgt tatcgcgaat gcccgaaaat tgatgaggcc atcaccctgg agctggagcg
     4021 caacacgcgt accggtaagc acgcccgctt tctgccgacg gttcacagcg acgcctgtac
     4081 cggttgcggt aagtgcgaaa aagtatgcgt gctggaacaa ccggcaatca aggtgttacc
     4141 gctgtcactg gcgaaagggg agttaggtca ccattaccgc ttcggttggc tggaggggaa
     4201 caatggcaaa tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc
     4261 gcagtcaccg ttggctggtg ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt
     4321 tgagcggtcc atggtttggt gtgtggatct tgcacggcaa ctacagcagt agcctgttat
     4381 tcgacaccgt tccgctgacc gatccgctga tgacgctgca aagtcttgcc agtggtcatc
     4441 tgcctgccac ggtggcgttg accggtgcgg tgattatcac cgtgctctac gccctggcgg
     4501 gaaagcgatt attttgcagc tgggtctgcc cgctgaaccc gattaccgac ttagcgaact
     4561 ggttacgcag gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac attcgctacg
     4621 tgctgttggt ggtcattctg ctgggatcgg ccttaactgg cacgctcatc tgggaatgga
     4681 tcaaccccgt atctctgatg gggcgcagcc tggtaatggg cttcggcagc ggcgcgctgc
     4741 ttattctcgc gctgttttta tttgatctac tggtcgttga acacggctgg tgcgggcaca
     4801 tttgtccggt tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg
     4861 ccaccgatcg gcagaaatgt aaccgctgta tggattgttt tcatgtttgc ccggaaccgc
     4921 atgtgctacg tgccccggtg ctggatgaac aaagcccggt gcaggtcacc agccgcgatt
     4981 gcatgacgtg cggtcgctgc gtggatgtct gttctgagga tgtatttaca ataactacac
     5041 gatggagttc gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg
     5101 gacggcgatg ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt
     5161 tagccagtcg ccggaagtat ccgggacaca ggaaggggcc attcggatgc caaaagagca
     5221 ggatcggatg ccgctgaact atgtgaatca gccgccaatg atcccgcata gcgttgaagg
     5281 ttatcaggta acgaccaata ccaaccgctg cttgcagtgc cacggtgtcg aaagttatcg
     5341 caccactggc gcgccgcgta tcagtcctac tcactttatg gacagcgacg gcaaagtggg
     5401 cgcggaagtg gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc aggccgatac
     5461 cgcgccaatc gtggggaata cctttacccc atcaaaaggt tacgggaaat aagaggttat
     5521 tatgggaaat tctgaccgta agcctggtct gattaagcgc ctgtggaaat ggtggcgtac
     5581 ccccagccgt ctggcgctgg ggacgctgct gttgatcggt tttgttggcg gcatcgtctt
     5641 ctggggtggc tttaacaccg ggatggaaaa agccaatacc gaagagttct gcattagctg
     5701 ccacgaaatg cgcaacacgg tgtatcagga atacatggat tccgtgcact acaacaaccg
     5761 tagcggcgtc cgtgcgacct gtccggattg tcacgttccg cacgagtttg tgccgaagat
     5821 gatacgcaag ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg ttattgacac
     5881 gccgcagaaa tttgaagctc atcgtctgac gatggcacag aatgagtggc ggcgcatgaa
     5941 ggacaataac tcgcaggagt gccgtaactg tcacaacttc gagtatatgg atacaaccgc
     6001 ccagaaatcg gttgccgcga agatgcatga ccaggcggtg aaagatgggc aaacctgtat
     6061 tgattgccat aaagggatag cgcacaagct gcccgatatg cgtgaagtcg agccaggttt
     6121 ttaacagggt tattgcgtgg gtatgcttga agccagagag ttactttgtg agcgggatga
     6181 acgaacctta tttagtggct tgtcatttac gctgaacgca ggagagtggg tacaaatcac
     6241 cggtagcaac ggtgcgggga agacaacgct tctccgtttg ctgacggggt tgtctcgccc
     6301 tgacgcaggc gaggttctct ggcaagggca gcccttgcat caggtacgcg acagctacca
     6361 tcaaaacctg ttatggatag gccatcagcc ggggatcaaa acccggctga cggcgttaga
     6421 aaatctgcac ttttatcatc gcgatggcga taccgcacaa tgtctggaag ccctggcgca
     6481 ggccgggctt gccggattcg aagatattcc tgtaaatcag ctctcggccg ggcaacaacg
     6541 ccgcgtcgct ttagcgcgtc tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc
     6601 ttttaccgcg attgacgtta acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac
     6661 ggagcagggg gggattgtga ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa
     6721 aattcgccgc atttcactga cgcaaacgag ggccgcatga tgttctggcg cattttccgt
     6781 cttgagctgc gtgtagcgtt tcgccatagc gccgaaatcg ccaacccgct gtggttcttc
     6841 ctgattgtaa ttaccctttt tccgctcagt atcggtccgg agccgcaact gctggcgcgt
     6901 attgcaccgg gcattatctg ggttgctgcg ctgctttcat ccttgctggc gctggaacga
     6961 ctgttccgtg acgatttgca ggacggcagt cttgaacaat tgatgttgtt gccgttaccc
     7021 ttgcccgccg ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta
     7081 ctcatccttt cgccactggt agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg
     7141 atggcgctga cgctgctgct gggaacgcct acgcttggct ttctcggtgc accgggcgtg
     7201 gcgctgacag tgggacttaa gcgcggtggt gtgctgctca gcatactggt gttaccgctg
     7261 actatcccat tactcatctt cgccaccgcc gcgatggacg cggcttccat gcatttgccc
     7321 gttgacgggt atctggcaat tttaggcgcg ttgctggcag gcaccgcgac attaagtcct
     7381 tttgcgacgg cggcagcgtt acgaatcagc attcaataac gctggattac tgatttttgt
     7441 tcgagtctgg tatcgaaact atgtggaaaa cactgcatca actggcgatc ccaccacggc
     7501 tgtatcaaat ctgtggctgg tttataccgt ggctggcaat tgccagtgtg gtcgtgctta
     7561 ccgtcggctg gatctgggga ttcggctttg ctccggctga ttatcagcag ggaaatagct
     7621 accgcattat ctacctgcat gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa
     7681 tggcggtggc ggcttttatt ggccttgtct ggcagatgaa aatggccaac ctggcggtgg
     7741 cggcgatggc ccccattggt gccgtgttta cctttattgc cctggttacc ggctctgcat
     7801 ggggaaaacc gatgtggggc acctggtggg tatgggatgc acgtctgact tctgaactgg
     7861 tgctgctgtt tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc
     7921 tggcgggccg tgcggcaggt atcctggtgc tgattggcgt ggtgaatctg ccgattattc
     7981 attactccgt ggagtggtgg aacaccctgc atcagggatc aacgcggatg cagcaaagta
     8041 tcgatccggc gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg ctgctgtctg
     8101 ccacgctgac gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt
     8161 gggtgagtga actgatactg aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa
     8221 tgaatttttc gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt
     8281 tattccgctg gtggttttgg tcgtgcactc ggtgatgcag catcgcgcaa ttctgcgtgg
     8341 tgtggcgcaa cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc
     8401 atgaatattc gccgtaaaaa ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg
     8461 ctgactatcg gtctggtgct atatgcgctg cgctcgaata tcgatctctt ttatacgccg
     8521 ggggaaattc tctacggcaa gcgtgaaact cagcaaatgc cggaagtcgg gcagcgtctg
     8581 cgcgtcggcg ggatggtgat gccgggtagc gtgcagcgcg atcccaattc gctaaaagta
     8641 actttcacca tttacgacgc tgaaggttca gtagatgtct cttacgaagg cattttgccg
     8701 gatctgttcc gtgaagggca gggcgttgtg gtgcagggcg aactggaaaa aggcaatcat
     8761 atcctcgcaa aagaagtgct ggcgaaacac gacgaaaact acacgccgcc agaagttgag
     8821 aaagcgatgg aagccaatca ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga
     8881 tgccggaaat tggtaacggg ctgctgtgtc tggcgctagg aattgcgctg ctgctgtccg
     8941 tgtatccgct atggggcgta gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt
     9001 ttgcctggct gctgtttatg tctgtggctg gcgcatttct ggtactggtc aatgccttcg
     9061 tggtcaacga cttcaccgtc acctatgttg ccagcaactc caatacccag cttccggtgt
     9121 ggtatcgcgt ggcggctacc tggggcgcgc atgaaggctc gctcctgctg tgggtgctgc
     9181 tgatgagcgg ctggaccttt gcggtagcga tttttagtca gcgtattccg ctggatattg
     9241 tggcccgcgt actggcgata atggggatgg tcagcgtcgg ctttttgctg ttcattctct
     9301 ttacctctaa cccgttctca cgcacgttgc cgaacttccc gattgaaggg cgcgatctta
     9361 acccgctgtt acaggatccg gggctgatct tccatccgcc tctgctctat atggggtacg
     9421 tggggttctc ggtggcgttt gcttttgcca ttgcttcttt gttgtgcggg cgtctggaca
     9481 gcacttatgc gcgttttact cgtccgtgga cgctggcggc gtggatcttc ttgacgctcg
     9541 gcattgtgct cggttccgca tgggcctatt acgaactcgg ctggggcggc tggtggttct
     9601 gggatccggt agaaaacgcc tcgtttatgc cgtggctggt ggggactgcg ctgatgcact
     9661 cactggcggt cactgaacaa cgcgccagct tcaaagcgtg gacattactg ctggcaatca
     9721 gtgccttctc gttgtgtctg ctggggactt tcctggtgcg ttccggcgtg ctggtatcgg
     9781 tacacgcgtt tgcctctgat ccggcacgcg gtatgtttat cctcgccttt atggtactgg
     9841 tgattggtgg ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa
     9901 acaatgcgct gtggtcgcgg gaatctctgc tgttagcgaa caacgttttg ctggtcgccg
     9961 cgatgctggt ggtattactg gggacgctgc tgccgctggt gcacaagcaa ctgggactgg
    10021 gcagtatttc gattggcgaa ccgttcttca acaccatgtt tacctggctg atggtgccgt
    10081 ttgcgctgct gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa
    10141 tccgcaattt attgattatc gccttcatct caacgctggt gctgtcgctg ctgctgccgt
    10201 ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg gcctgctgga
    10261 ttgctgtgct ggcaattgcg gaagctgcgc tgcgtatttc acgtggcacg aaaaccacct
    10321 tcagttattg gggaatggtg gcggctcacc tggggctggc agtgacaatt gttggtattg
    10381 cctttagcca gaactatagc gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg
    10441 atattcatga atatcgcttc accttccgtg atgtcaaaga ggtgactggc ccgaactggc
    10501 gtggcggtgt ggcgactatc ggcgtaacgc gcgatggcaa gccggaaacg gtgctgtatg
    10561 cggaaaaacg ttattacaac actgccgggt cgatgatgac cgaagcggca attgacggcg
    10621 gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct gga
//
LOCUS       DAMZII010000161        10528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZII010000161 DAMZII010000000
VERSION     DAMZII010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.161"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            100..675
                     /gene="ssuE"
                     /locus_tag="POA77_004157"
     CDS             100..675
                     /gene="ssuE"
                     /locus_tag="POA77_004157"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDI6367508.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            668..1627
                     /gene="ssuA"
                     /locus_tag="POA77_004158"
     CDS             668..1627
                     /gene="ssuA"
                     /locus_tag="POA77_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDI6367509.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1624..2769
                     /gene="ssuD"
                     /locus_tag="POA77_004159"
     CDS             1624..2769
                     /gene="ssuD"
                     /locus_tag="POA77_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDI6367510.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2780..3571
                     /gene="ssuC"
                     /locus_tag="POA77_004160"
     CDS             2780..3571
                     /gene="ssuC"
                     /locus_tag="POA77_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDI6367511.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VGTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3568..4335
                     /gene="ssuB"
                     /locus_tag="POA77_004161"
     CDS             3568..4335
                     /gene="ssuB"
                     /locus_tag="POA77_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI6367512.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4542..7154)
                     /gene="pepN"
                     /locus_tag="POA77_004162"
     CDS             complement(4542..7154)
                     /gene="pepN"
                     /locus_tag="POA77_004162"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDI6367513.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7420..8622
                     /gene="pncB"
                     /locus_tag="POA77_004163"
     CDS             7420..8622
                     /gene="pncB"
                     /locus_tag="POA77_004163"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI6367514.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8791..10191
                     /gene="asnS"
                     /locus_tag="POA77_004164"
     CDS             8791..10191
                     /gene="asnS"
                     /locus_tag="POA77_004164"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI6367515.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2399 a         2709 c         2816 g         2604 t
ORIGIN      
        1 cacaaaattt atatttggca ttttcttgtc tctccgacag actgaccgaa ttgttatcaa
       61 tgttaacaaa aaaagaacaa ttggttataa ggagagagta tgcgtgtcat caccctggcg
      121 ggtagtcctc gctttccttc tcgctccagc tccttgctgg aatatgcgcg ggaaaaacta
      181 aatggcctcg atgtagaggt ttatcactgg aatctgcaaa acttcgcccc ggaagatctg
      241 ctttatgctc gtttcgatag tccggcactc aagaccttca ccgaacagct gcaacaggct
      301 gatggactga tagtcgctac acctgtgtat aaagccgcct attccggcgc gctgaaaacc
      361 ctgctcgacc tgctgccaga gcgcgctttg caaggcaaag tggtgctacc gctggcgacg
      421 ggcggtaccg tggcccatct gctggcggtc gattatgccc ttaaaccagt tttaagcgca
      481 ctgaaagctc aggagatcct gcacggcgtg tttgccgatg actcacaagt aattgattac
      541 catcacagac cccagttcac gccaaatctg caaacccgtc ttgataccgc gctagaaact
      601 ttctggcagg cattgcaccg ccgcgatgtt caggttcctg accttctgtc tctgcgaggt
      661 aatgcccatg cgtaacatca ttaaactggc gctggtggga ttgcttagcg tctctacgat
      721 tgcggttgct gcagaatcct cgcctgaagc gttacgtata ggctatcaga aaggcagtat
      781 tggtatggta ctggcaaaaa gccaccagtt actggaaaaa cgctatccgc aatcaaaaat
      841 ctcctgggtg gagttccccg cgggtccgca aatgttggaa gcgttaaacg ttggcagtat
      901 tgatctcggc agtaccgggg atattccgcc aatctttgcc caggctgccg gggctgattt
      961 ggtatatgtg ggcgtcgagc caccgaagcc caaagccgaa gtgattctgg tggcagaaaa
     1021 cagcccgatc aaaaccgtag ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc
     1081 cagttcacac aaccttttac tgcgtgcact gcgtcaggcc ggacttaagt ttaccgatat
     1141 ccaacccact tacctgacgc ccgctgatgc ccgcgccgcg ttccagcaag gtaacgttga
     1201 cgcctgggct atctgggatc cctactactc tgctgcatta ttacagggcg gcgtgcgggt
     1261 gctgaaagac ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtcccta
     1321 tgcagaaaaa aacggcgctt ttattcaggg cgtactcgca acctttagtg aggccgatgc
     1381 gttaacccgc agccagcgcg agcaaagcat cgctttactg gcaaaaacga tgggcttacc
     1441 ggcaccggtg attgcctctt acttagatca tcgccctcct accaccatca aaccggttaa
     1501 cgccgaggtt gccgccttac agcagcaaac ggcagatctg ttttatgaaa atcgtctggt
     1561 gccgaaaaaa gtcgatattc gccagcgcat ctggcagccc actcaactgg aaggaaaaca
     1621 attatgagtc tgaatatgtt ctggttttta ccgacccatg gtgacgggca ttatcttgga
     1681 acggaagaag gttcacgccc ggttgatcac ggctatctgc aacaaattgc gcaagcggcg
     1741 gatcgtctgg gctataccgg tgtgctgatc ccaacggggc gatcgtgtga agatgcgtgg
     1801 ctggtggcgg cgtcgatgat cccggtgacg cagcggctga agtttcttgt cgccctgcgc
     1861 cccagcgtaa cctcacctac cgttgccgct cgccaggccg ccacgcttga ccgtctctca
     1921 aatggtcgtg cgttgtttaa tctggtcaca ggcagcgatc cacaagagct ggcaggcgac
     1981 ggcgtgttcc ttgatcatag cgagcgctac gaagcctcgg cggaatttac ccaggtctgg
     2041 cggcgcttat tgctggggga aaccgtcaat ttcaacggta aacatattca tgtgcgcgga
     2101 gcaaaactgc tcttcccgcc gattcaacag ccttatcctc cgctttactt tggcggatcg
     2161 tcagacgtcg cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa
     2221 ccgccagaac tagtaaaaga gaagatcgaa caagtgcggg cgaaagctgc cgcgcatgga
     2281 cgcaaaattc gtttcggtat tcgtctgcat gtgattgttc gtgaaactaa cgacgaagcg
     2341 tggcaggccg ccgagcggtt aatctcgcat cttgatgatg aaactatcgc caaagcacag
     2401 gccgcattcg cccggacgga ttccgtagga caacagcgaa tggcggcgtt acataacggc
     2461 aagcgcgaca atctggagat cagccccaat ttatgggcgg gcgttggctt agtgcgcggc
     2521 ggtgccggaa cggcgctggt gggcgatggt cctacggtcg ctgcgcgaat caacgaatac
     2581 gccgcgcttg gcatcgacag ctttgtgctt tcgggctatc cgcatctgga agaagcgtat
     2641 cgggtcggcg agttgctgtt cccacatctg gatgtcgcca ttccggaaat tccccagccg
     2701 cagccgctga atccgcaagg cgaagcggtg gcgaatgatt ttatcccccg taaagtcgcg
     2761 caaagctaag gaagaaataa tggcaacgcc agtgaagaag tggttattgc gcgttgcccc
     2821 ctggttttta ccggtgggca tcgtggcggt gtggcaactg gcctcctcag ttggctggct
     2881 gtcgacgcgt attttgccct caccggaagg ggtggggacg gcgttctgga cgctctccgc
     2941 cagcggcgaa ctgtggcagc atctggcgat cagttcctgg cgggcgctga ttggcttttc
     3001 aattggcgga tcgctggggt tgattttggg actgattagc gggctatctc gctggggaga
     3061 acggctgctg gatacctcaa ttcagatgtt gcgcaacgta ccgcatctgg cgctgatccc
     3121 gctggtgatt ttgtggtttg gcattgatga atccgcaaaa atctttctgg tggcgctcgg
     3181 cacgctattc cccatttata tcaacacctg gcatggcatc cgtaatatcg atcgcggtct
     3241 ggtggaaatg gcgcgtagct atggattatc cggcataccg ctgtttatcc atgtgatcct
     3301 gcctggtgcc ctgccctcaa ttatggtcgg cgtgcgtttt gcgttgggcc tgatgtggct
     3361 gacgctgatt gttgccgaaa ccatttctgc caattcaggc attggttatc tggcgatgaa
     3421 tgcgcgggag tttttgcaaa cggacgtggt ggtagtcgct attattcttt acgccctgct
     3481 cggcaaactt gctgacgtca gcgcgcagct gttagagcgc ctctggttgc gctggaaccc
     3541 ggcttatcat ttgaaggagg ccacggtatg aatactgctc gtctgaacca gggcacacca
     3601 ttgttgctca atgcagtaag caaacattac gcggaaaata tcgtcctgaa ccaactggat
     3661 ttacatattc cggcaggtca gtttgtggcg gtggtgggcc gcagcggtgg tggcaaaagt
     3721 accctgctgc gcctgctggc cgggctggaa acgccaaccg caggcgatgt gttggcaggc
     3781 accacaccgc tggctgaaat tcaggaagac acgcgaatga tgtttcagga tgcgcgtctg
     3841 ctaccgtgga aatcggtgat tgataacgtt gggttaggcc ttaaaggcca gtggcgcgat
     3901 gccgcgcgtc gggcgttggc tgcggtagga ctggagaatc gcgccgggga atggcctgcc
     3961 gcactttcag gcgggcagaa acagcgagtg gcgctggcaa gagcgttgat tcatcgaccg
     4021 ggtttattgt tgcttgatga accgctcggg gcactggacg ccttaacgcg actcgagatg
     4081 caggatttga ttgtgtcact ttggcaggag cacggcttta ccgtactgtt ggtgacgcat
     4141 gatgtgagcg aagcggtagc aatggctgac cgggtgttgt taattgaaga gggaaaaatt
     4201 ggtctggatt tgacagtgga tatcccccgt ccgcgtcgat tagggtcggt gcgattagcg
     4261 gagctggaag cggaagtatt gcaacgggta atgcggcgtg ggcattctga acaactgatt
     4321 cggcgtcatg gttaatatgc cggatgtggc gcacaaaacc agcattttca acggactagt
     4381 aggcctgata agacacgtca agcatcgcat caggcagttg ggcacggctg ccggatgcgg
     4441 cgtgaacgcc ttatccagcc tacaaaacca gcaatttcaa cggagttgta ggcctgataa
     4501 gacgcgtcaa gcgtcgcatc aggcattttg gctcaaaagt atcaagccag tgctttggtg
     4561 atcttctcgt acagatcgcc agagagattt tccagccctt tcaactgttc cagcgccgcg
     4621 cgcattttct cctgacgttt ggcatcataa cgtttcaggc gaatcagcgg ttcaatcaga
     4681 cgtgaagcta cctgcgggtt acggctgttg aggtcggtaa gcatttccac caggaactgg
     4741 taaccgctgc catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc
     4801 aacgaacgaa tacggttcgg attgctcatg gtaaatgagc gatgctgcaa cagaccacgc
     4861 accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc
     4921 accagaccgt cctgatgcca cttatcgtcg tactcctgca tcagcgcgtc acggcaaggc
     4981 agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca
     5041 tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc
     5101 aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacacg gtactcgctc
     5161 tggtaattcg cgttgtaaat agccagcagc tcatccgcca gttcagtcgc cagagtacga
     5221 gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc
     5281 atttcattga cagaaggcag cgtcaggatt tctgccgcca gcgccggatc aatcttctca
     5341 tcgagcagca ccgcgcggaa agcgtcagcc acatgtaccg gcagagacag cggctgccct
     5401 tgctgatgac gggcgacgtt cagcttgatg taggttgcca gcaaactttg cgccgcatcc
     5461 caacgggaga aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac
     5521 ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag
     5581 tagacattat caaaaacaaa ggtctgttcc gcctgggtga cgttcagcac ggaattcacc
     5641 ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tgtcatacaa ttcgatggca
     5701 aatggaatat gcagcggctg tttttctgcc tgatccggcg tggctggcgt gcgctggctg
     5761 atggtcaggg tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt cacaatcggc
     5821 gtgccggact ggctgtacca acggcggaaa tgggagagat cgacattcga cgcatcttcc
     5881 atcgcctgca caaagtcgtc gcaggttgcc gcgctgccat catggcgctc gaaatagagt
     5941 tgcatccctt tctggaagtt ttcttcgccc agcagggtgt ggatcatgcg aatcacttcc
     6001 gcgcccttct cgtaaacggt cagggtgtag aagttgttca tctcaatgac catatccggg
     6061 cggatcgggt gcgccatcgg gctggcgtct tccgcaaact gcaatccgcg catggtgcgc
     6121 acattattga tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg
     6181 aagacggtta aaccttcttt caggctgagc tggaaccagt cgcggcaggt cactcggtta
     6241 ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag ataatctttg
     6301 tcggtggcgg tgtcggtgcg ggccaacaca tatttggagt taaagatatt cagcccctta
     6361 ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca
     6421 tactccaggc caaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc
     6481 cacggcgcgc gatcaaggtt gccgcgatcg acgtacagct ccagtgctac ttcgcgacca
     6541 gaacgcgtgg taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg
     6601 tagcacggtt tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt ttcgagttcg
     6661 ccttgcgcaa cgcggttacc attggaaagc aggaagggat atttgatttt atcggcaata
     6721 attttggtgg taaaacgcgc cagcacgtcc gggcggtcga gataatacgt aatatggcgg
     6781 aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc
     6841 gccgtattcg ccgccgggct tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa
     6901 ttactgatga ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc atcattaata
     6961 tgaacagaaa ccagtttgag gtcttcgccg ttgagacgaa gcggagcatc tgatgcacca
     7021 tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc
     7081 aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt
     7141 ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc
     7201 ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg
     7261 ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg
     7321 atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt
     7381 gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct
     7441 gttctgcact cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt
     7501 catcactatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg
     7561 ggtatttatg ccgatgctat tcgtgaacag attcaggcga tgcagcacct gcgcctgcag
     7621 gatgatgaat atcagtggct ctctgctctg cctttcttta aggccgacta tcttaactgg
     7681 ttacgcgagt tccgctttaa cccggaacaa gtcaccgtat ccaacgataa tggcaagctg
     7741 gatattcgtt taagcggccc gtggcgtgaa gtcatcctct gggaagttcc tttgctggcg
     7801 gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc
     7861 gacacgctgg aaagcaaatt agtcgacttc tcggcgttaa ccgccggcct tgatatgtcg
     7921 cgcttccatc ttatggattt tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc
     7981 atcgttaagc gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg
     8041 cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcacatcag
     8101 caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagaa
     8161 tatcccgacc aacttggcat tgcattaacc gactgcatca ctatggatgc tttcctgcgt
     8221 gatttcggtg tcgagttcgc cagtcgctat cagggcctgc gtcatgactc tggcgacccg
     8281 gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tgggaattga tccacagagt
     8341 aaaactctgg ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac
     8401 ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc
     8461 caggtaaaac ccctgaatat tgtgattaag ttggttgagt gtaacggtaa accggtggcg
     8521 aaactttctg acagccctgg caaaactatc tgccacgata aagcgtttgt tcgggcgctg
     8581 cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat
     8641 cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg
     8701 tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta
     8761 ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc
     8821 cagggccgcg tagccgttga cagcgaagtc accgtacgcg gatgggtacg tacccgccga
     8881 gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta
     8941 caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg tctgaccacc
     9001 ggctgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt
     9061 gaaattcagg ccagcaaagt tgaagttgct ggttgggttg aagatccaga cacttacccg
     9121 atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc
     9181 acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc
     9241 ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattaccgc atctgatacc
     9301 gaaggtgcag gcgaaatgtt ccgcgtttct acgctggatc tggaaaacct gccgcgtaac
     9361 gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta
     9421 tctggccagt tgaacggcga aacctacgct tgcgcattat ccaaaattta taccttcggc
     9481 ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg
     9541 gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga agccatgctg
     9601 aaatatgtct tcaaagcggt tctcgaagaa cgcgcagacg atatgaaatt cttcgctgaa
     9661 cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaggccga tttcgcgcag
     9721 gtggattaca ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg
     9781 gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt
     9841 aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctc
     9901 aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat cggtgagatc
     9961 attggtggct cccagcgtga agaacgtctg gacgttctgg acgagcgtat gctggaaatg
    10021 ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg
    10081 cattcaggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg cgtgcaaaac
    10141 gtgcgtgatg tgattccgtt cccacgtact ccgcgcaacg ccagcttctg attttcttcc
    10201 tcaaagagcc agcgcctgct ggctcttttt tatcctttct gtcaagttat ctgtttgtta
    10261 agtcaagcaa tctatttgca accccgccat aacgatcatc ctgttacgga atattacatt
    10321 gcaacattta cgcacaaaaa ataacccgca ttcttattgc ggattagttt tttcttagct
    10381 gatagcacaa ttttcatact attttttggc attctggatg tctgaaagag gattttgtgc
    10441 caggtcgata aagtttccat cagaaacaaa atttccgttt agttaattta aatataagta
    10501 aatcatataa atagataaaa attgctgt
//
LOCUS       DAMZII010000162        10441 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZII010000162 DAMZII010000000
VERSION     DAMZII010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.162"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            2..784
                     /locus_tag="POA77_004165"
     CDS             2..784
                     /locus_tag="POA77_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI6367516.1"
                     /translation="MASVLISAVIATAGSVYITSKYLKPDVPGNDEIGRVAASYLVKN
                     PQYLLEAGKALEKENTNASLERIIPYAPALFETKETPNIGPDNAAVAVIEFFDYQCHF
                     CMQVAPVVESVLSQSTDVKFFFKEFPIFAGSKPVSAMGAATGLHVYQTFGAEAYRKYH
                     NNLMTSAYVFFNNQRAFTLNDLDMVVNKSGFNSSFGDREKSRYENVISGNMQLGEALG
                     INGTPGFIIMNMQKPDAATTSFIPGAVDEATLKYAIQKARGG"
     gene            complement(836..2095)
                     /locus_tag="POA77_004166"
     CDS             complement(836..2095)
                     /locus_tag="POA77_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI6367517.1"
                     /translation="MYMHVDINGAYAAFECAMDPKLAKSPLIIASNNDATIIAMNRLA
                     KNTGLKRGMPIFKCRDLIEQHHIAVRSSNFTLYEDYSNRFHETLEGFAEDTDRYSVDE
                     SFMLLKNMDKIVDFEEYGCLIRRTLLHNLSLTCGIGVSTTKTLAKLCTYASKRWAATG
                     GVVVLTDPFRIKKLLSLISTREIWGIGRKISERLAAYGIITACDFYDSDVRFLRKAFG
                     VEMERTWRELHGESCFRLHESPPVRQQIIVSRSFGQRLNTFSKMHEAVSFFTGRAAEQ
                     LRNDGSWTRQITVFIQSSPFARAETRYSSCGIEPLTATQDTRDLVSAAGAILTRIYKP
                     GIDYAKAGVMLSSMTDGTEQLSLFDERPARRGSEALMKVMDRFNKEHRGSLFLACEGI
                     HQDFRGKQAMLSPRYTTRWNELLVVKA"
     gene            complement(2079..2513)
                     /gene="umuD"
                     /locus_tag="POA77_004167"
     CDS             complement(2079..2513)
                     /gene="umuD"
                     /locus_tag="POA77_004167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDI6367518.1"
                     /translation="MSVFPERFASPPRDTTPFFSDLLSCGVMSPCAGHEDNELNLHDF
                     VVRNRPATFFVRASGLSMVDAGINDGAILVVDRSLTAEHGSIVVALVDGEFTVKILQT
                     FPKLMLMPSNPAFKPIPVNPDTLEIWGVVTFALNQFPHVHAR"
     gene            2691..3305
                     /locus_tag="POA77_004168"
     CDS             2691..3305
                     /locus_tag="POA77_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367519.1"
                     /translation="MAIDTDDNDPVDAEACEKYLAQLRELEAYRAYRTTAAIDWFFDQ
                     ATRAIHGELWLAACTTFLNGIETSLRVTMKLKASQAQPQAPTPLVDLSDMATLSNALL
                     RRAHQAGMPVTLLAFPDEQDLLTKIADGAPKLSYAEIVRVRHNLCHGNILEHIITVSD
                     GMGEPVRLFTPECMRDLAQTLSAVSKVWIAGLHQYWCDNNLSMP"
     gene            3453..3806
                     /locus_tag="POA77_004169"
     CDS             3453..3806
                     /locus_tag="POA77_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367520.1"
                     /translation="MKIKNIVLAGIISLVFSVHASDKQNIKRDPIPYLGYDAVTSNMC
                     IDAGGLTDPVEMRKKATETLLMILHKQPGDKLSKQSEDTIEAFVTRTRSSSDDISKCQ
                     FSVYERYWEIVQPNK"
     gene            4082..7417
                     /locus_tag="POA77_004170"
     CDS             4082..7417
                     /locus_tag="POA77_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367521.1"
                     /translation="MGDLVSKNNIDRLERFHSLLAGQYWSALVDIPAEAIVAGDTLLI
                     NSLRYVDNNLHTVILRAHPRHYGEWVSVEIVAENGSKSTKDKRLNEHRFLTADFLALF
                     EYQPDHEIIRQSELKGIQDEVADLQMRLTETLQDPSALRELAIKRVEDEEASKRGGAT
                     EDRMLPAVRNAEDVAVTMALGTVQNTLSQGISEAQVSLMAKVAEREGKIAGAVANIIT
                     SRTGAITRALEKMHPYYAELAAAKLAGTQEGIEQASKIQAGVLTLELFVGKNVTVVDV
                     AEGEPADSSIPLTLCQKKLVVDEEFSAWSDIDEHFDFRSLSAFLKALKDNQGLVEQIF
                     PTERCVLVMVTTRRFIDYGDTWANAENNEKNSIVFLMVRNGQNIKQIYSPVESHLGAS
                     RLFPSEDEQQGHFRGFDGTTIKFEDVAYTDRLKAHDLMALHYRRLLIMLFGLDQRLAL
                     FGQFYPQHEKANFLNLSFQERYFHFLHDEDGTGLLASPDSQTLDEFISEKNSYIQPGS
                     RLLCNWRELMTPRTAPGAVKEDSGYSGYSFIADPEDNVSAAVVYRQGDTLAVDVAVKR
                     FSTDKMFNCKVNVSAYSPWRRGEAELAYLCLDAVGPEELTRFIQQRKFRSNHLFYIRF
                     FKAAIKFLEQERENELPHRQYLLAAMQDSGMHLPGNIQELIHQCIASWKTGNRGVSLA
                     VGMSTEKGRQALLNQLYRLTQGASEMVSVIQEHVAASGSQLLRAGVNSSGNYVAFIAP
                     KPYECDNRLEAHAWVHRVVYATGKRNIRETGRSWVSMPERSASEITLWEDEEQSRKWY
                     SMTPVFSSWTEKQKLFEMCEKGAGLLKGAMGTPDDEEYGDLLEMWGDAYIACNASGEH
                     VTTPDMFLPVGLIKSRKSLKLIALGTCSTEHWMYARAGDDDSRELLLELYTSWYEYPD
                     KARARLLSMAEKNSGLRFVLLEGKSRKLERFAVKRPEEIVNWHAGDLKEIPTMLNDQW
                     ACHMAMVSQNDKVYLTPDLLDDDGLPNFNGVTRQVPGEGYQPVNVYEFESDYFNAVYD
                     ADGKKVALCHWYDVTDNSYSAEELIGNMPHNAFKFLQYRLDNIEQAEAFIKYRNRNYQ
                     PRENADWPEPPEGVKRYVIRARS"
     gene            7713..7934
                     /locus_tag="POA77_004171"
     CDS             7713..7934
                     /locus_tag="POA77_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367522.1"
                     /translation="MPKKNLYSEEIVIAKKQLTQLSTLKDKVSLLTRQWEGDIGANLP
                     GYTELLAHVERIERQIHEQIGSWKKTPAM"
     gene            8184..8570
                     /locus_tag="POA77_004172"
     CDS             8184..8570
                     /locus_tag="POA77_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367523.1"
                     /translation="MGVDMNYEFQKKSPKGWDRVNDNFSNDRSYLLYSWLGLDARNTW
                     GVAAITPLRGLPDDIELQWDEDGCDDYWGEHSQTWLLSDEILASTSPVAIEDDEPGSV
                     VAEFCAEVQRLHGLHGTVRIVLGFTG"
     gene            complement(8582..9007)
                     /locus_tag="POA77_004173"
     CDS             complement(8582..9007)
                     /locus_tag="POA77_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367524.1"
                     /translation="MTDQASIPVDTPVLALATDAYSSLKNILNDNGTSDTTGTCMFAS
                     LLVCEFAHRRGMSAAVRGGNGTDDGGIFNESGGHGHYWCEVSAGEMIFYIDIAAEQFG
                     YPSFIIKNANDVSGWPRYIPGDQVTVDEHVRITLSGGIR"
     gene            complement(9041..9514)
                     /locus_tag="POA77_004174"
     CDS             complement(9041..9514)
                     /locus_tag="POA77_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367525.1"
                     /translation="MGSIKELLFDIQEEWRHEWISINYPEAEEETLEWDAAAQEYSWF
                     RDWMEEAAEQQHFEASLNCIPERLQEALDELHELQGLLDTEQLIVSPNLLSELKNLSI
                     QEGYMLKIENVLPPNFRVFLVREGFIFPGESWVCGSGYWLPESEVLKNGINSLLV"
     gene            complement(9597..9887)
                     /locus_tag="POA77_004175"
     CDS             complement(9597..9887)
                     /locus_tag="POA77_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367526.1"
                     /translation="MEISISLDVLFEALMILGHGRVWMQEMEFEHLTGYSCYRELRTW
                     DNRIVAWWSDADDTWYLCVEVDVITAVPSHIPNKKGELSEEVCFQLMALGRY"
BASE COUNT         2810 a         2371 c         2594 g         2666 t
ORIGIN      
        1 cgtggcgtct gttttaattt ctgcggtgat tgcgacggct ggctctgtgt atattacgtc
       61 gaaatatcta aaacccgatg taccgggtaa cgacgaaata ggacgagtgg ctgcttccta
      121 tctggtaaaa aatccccagt atcttctcga agcaggtaag gcgctagaaa aagaaaatac
      181 taacgcttcc cttgagcgga ttatccccta cgcaccggca ctcttcgaga ccaaagagac
      241 acccaatatc ggacctgata atgcggccgt cgctgttatt gagtttttcg actaccagtg
      301 tcatttctgt atgcaggtag cgccggttgt tgaatctgtg ttgagccaaa gcactgatgt
      361 aaagttcttc tttaaagaat ttccgatctt cgctggctca aaacctgttt ccgcaatggg
      421 ggcggcgaca ggcctgcatg tgtatcagac attcggtgcg gaagcgtatc gtaagtatca
      481 caacaatctg atgaccagtg cgtatgtatt cttcaacaac cagcgtgcgt ttacactcaa
      541 tgatttagac atggttgtga acaaatccgg gttcaacagc tcattcggcg atcgggagaa
      601 aagccgctat gagaacgtta tctccggaaa catgcagctg ggggaggctc tcgggattaa
      661 cggtacgcca gggttcatta ttatgaacat gcaaaaaccc gacgctgcaa cgacgtcatt
      721 catcccgggc gctgttgacg aggccaccct gaaatacgca atccagaagg cccgtggagg
      781 ctaaagttac ttttccttcc gaaactggcc atgccgggta acaccggcat gaacgtcagg
      841 ctttgacgac aagtaactca ttccagcgag tggtataccg tggggacagc atggcctgct
      901 tcccccggaa atcctgatga atcccttcgc aagccagaaa cagagagcct cgatgctctt
      961 tgttaaaccg atccatcacc ttcatcagtg cttcactgcc tcgcctggcg ggacgttcat
     1021 cgaacaggga tagctgctca gtgccatcgg tcattgacga aagcatcacg cccgccttcg
     1081 cataatctat gccgggtttg tagattcggg tcagtatggc gcctgccgca ctgaccaggt
     1141 cgcgggtgtc ctgcgtcgca gtaagcggct caataccgca gctgctgtac cgggtttcag
     1201 ctctggcaaa cgggctgctc tggataaaca cggtgatttg gcgagtccag gagccatcgt
     1261 ttcgaagctg ttcagcggca cggccggtaa agaatgacac tgcctcatgc atcttgctga
     1321 atgtattaag gcgctggcca aagctccgcg agacgataat ttgttgtctg acaggagggg
     1381 attcatgcaa ccggaaacag gactcgccat gcagctcccg ccaggtacgt tccatttcaa
     1441 caccgaatgc ctttcgtaaa aaccgcacat cgctgtcata aaagtcgcag gccgtgatga
     1501 tcccgtacgc agccagccgc tcactgattt tgcggccaat gccccagatt tcccgggtac
     1561 tgatgagact cagcagtttt ttaattcgga atgggtctgt aaggacgacc acaccaccag
     1621 ttgctgccca tcgcttactg gcgtaggtgc ataattttgc gagcgttttc gtggtcgata
     1681 ctccaatccc gcaggtgagc gaaagattgt gtagcagcgt tcgtctgatc aggcatccgt
     1741 actcttcaaa atctactatt ttgtccatgt ttttcagtag catgaagctt tcatccacgc
     1801 tgtaacgatc ggtatcctcg gcaaacccct ccagcgtttc atgaaaccgg ttggaataat
     1861 cttcgtacaa cgtaaagttg gagctccgta ccgcaatatg atgctgctca atcagatccc
     1921 ggcatttgaa gataggcatc ccgcgtttga gccctgtatt tttggcgagc ctgttcatgg
     1981 cgataatggt ggcatcgttg ttgctcgcga tgatgagcgg ggatttggca agtttggggt
     2041 ccatggcgca ttcaaatgcc gcgtaggctc cattgatatc aacgtgcatg tacatgagga
     2101 aactgattca acgcaaaggt gacaacgccc cagatttcaa gagtgtccgg gttaacaggt
     2161 atcggcttga aggcggggtt ggagggcatc aacatcagtt tcgggaaggt ctgcaaaatc
     2221 ttcacggtaa actcgccgtc aaccagcgca acgacgattg agccgtgctc cgcagtcagt
     2281 gaccgatcca caaccagtat ggcgccgtcg tttattccgg cgtctaccat gctgagacct
     2341 gaggccctga caaagaaagt cgcggggcga ttacgaacca caaaatcatg taaattcagc
     2401 tcattgtctt cgtgtccggc gcacggactc atcaccccgc aactcagtag gtcactgaaa
     2461 aaaggggttg tatcacgagg aggggatgca aaacgttcag ggaatacaga catgagcaca
     2521 ctcggattca atactggata tttaaccagt atcaccagat aaagagaaaa gatcaaattt
     2581 caatggctca gcattcggga aagatatccc ggcactaagc cgtaatttat gtaacggtat
     2641 gatatggttc agcggatttt cattcacgaa catgacaaca ggaattccct atggctatcg
     2701 ataccgacga taacgatccg gtagacgcag aggcgtgcga gaagtacctg gcacagttac
     2761 gggaactgga ggcatacagg gcgtaccgaa cgacagctgc gattgactgg ttctttgacc
     2821 aggctacccg ggccattcac ggtgagttgt ggctagccgc ctgtacgact ttcctgaacg
     2881 ggattgagac ctcactgagg gtgacgatga agctgaaggc gtcgcaggca caaccgcagg
     2941 caccaacacc gctggtggac ttaagcgata tggccacact cagtaatgct ctgctaagaa
     3001 gggctcacca ggccggtatg ccggtgacac tgctggcctt tcccgacgag caggatctgc
     3061 tgaccaagat tgcggacggc gccccgaaac tatcctatgc ggaaatcgtg agggttcggc
     3121 acaatttatg tcacgggaac atcctggagc acatcatcac tgtgagtgac ggtatggggg
     3181 agccagtccg gttattcaca cctgaatgta tgcgtgatct ggcgcagacg ctgtcggcag
     3241 tctcgaaagt atggattgca ggcctgcatc aatattggtg tgataacaat ttatctatgc
     3301 cttgaaaatt cctgacggat tctgttcccg gtgtcatttg tacgccggga tgaaaaatga
     3361 tgcttcacaa caggaattag ctgtttattg cttagaaaga tttgtgatat aaaaattatg
     3421 ttttgaactt ggtgatagta atggagaaac atatgaaaat taaaaacatt gtattggcag
     3481 gaataatttc attggtattt tctgttcatg ccagcgataa gcagaatata aaaagggacc
     3541 caatacctta tttagggtac gacgcagtta cttcaaacat gtgcatagat gctggtggtt
     3601 taactgatcc agtggagatg agaaagaaag ctactgaaac attactaatg attcttcaca
     3661 aacaaccagg tgataaacta tctaaacaat cagaagatac aatagaagca tttgttacac
     3721 gaacaagatc ctcttcagat gacatatcta aatgccagtt cagtgtttat gaaagatact
     3781 gggaaatagt tcagccaaat aaataacata gatttgcgtg agatttataa tacaaaacgc
     3841 tcattaggtc tatgtcttta aaggcgcgtg gcttaatggg caattaaatc catactcctg
     3901 caatacatgc ttcaatcctc aactcgtttg ataatggctg tatttaattt gctggattcc
     3961 accatttaaa atggacaggc tctgttttaa atgaatcacc tgtttccgca tgccaggtta
     4021 tttgggtgaa cagccaatat ggtatgatgt ttataacaaa caaattcgga aggtatagtt
     4081 tatgggggac ctggtcagta aaaataatat cgatcgcctt gaacgatttc attctctgct
     4141 ggcagggcag tattggtctg cgctcgtcga tattccggct gaggcgattg tggccgggga
     4201 cactctcctc attaactcgt tgcggtacgt ggataacaat ctgcataccg tcatccttcg
     4261 cgctcacccc cggcattacg gggaatgggt aagcgttgaa atcgtcgctg agaacggttc
     4321 aaaatcaacg aaagacaaga gactgaatga gcatcggttt ctgacggccg atttcctcgc
     4381 gttgtttgaa tatcagcccg accatgaaat tatccgtcag tcggagctca aaggcattca
     4441 ggatgaggtc gctgacctgc aaatgcgtct tacggaaact ctgcaagatc cctctgcact
     4501 ccgggaactg gctattaaaa gggtcgaaga cgaagaggcc agtaaaaggg ggggggcaac
     4561 tgaggacaga atgcttccag ccgtcaggaa tgcagaagat gttgcagtca ccatggcgct
     4621 gggtacagtg cagaataccc tttctcaggg gatttcagag gctcaggttt ctctgatggc
     4681 gaaagttgcg gaacgggaag gcaagattgc cggtgccgta gctaacatta ttacgtcgcg
     4741 taccggcgcc atcacccggg cgctggagaa aatgcatccg tattatgcgg agctggccgc
     4801 tgccaagctg gccggaacgc aggagggcat tgagcaagcc agcaaaattc aggcaggtgt
     4861 tctcacgctg gaattattcg tcggcaaaaa cgtgacggta gttgatgttg cggaaggcga
     4921 gccagcagac agcagcatcc cgttgacgct gtgtcagaag aaactcgtcg tggatgagga
     4981 gttttctgcc tggagcgata tcgacgaaca ttttgatttc aggagtcttt ccgcattcct
     5041 caaggcgcta aaagataacc aggggctggt ggagcaaatt ttcccgactg agcgctgcgt
     5101 acttgtcatg gtcactaccc gcagatttat tgactatggt gatacctggg cgaatgctga
     5161 aaacaacgaa aagaacagca tcgtgtttct gatggtccgc aacgggcaaa atatcaaaca
     5221 gatctattcc ccggtggaga gccatcttgg ggcgtcccgg ttatttccct ctgaagatga
     5281 gcagcagggc cacttccgag ggtttgacgg taccaccatc aaatttgaag atgtggctta
     5341 taccgatcgt ctcaaagccc atgacctgat ggctctgcat taccgacgtc tgcttatcat
     5401 gctgtttggt cttgaccagc gactggctct ctttggtcag ttctatcccc agcatgaaaa
     5461 ggcgaatttc ttgaatctga gcttccagga acggtacttc cattttctgc acgatgaaga
     5521 cggcacaggg ctgctggcat caccagactc gcagacgctc gatgaattta ttagtgaaaa
     5581 aaactcctat attcaaccgg gctcgcggct gctctgcaac tggcgtgagc ttatgacccc
     5641 cagaacagca cctggtgccg ttaaagagga cagcggctat tcagggtact ccttcatcgc
     5701 tgacccagag gataatgtct ctgctgcggt tgtgtatcgt cagggagata ctctggccgt
     5761 tgatgtggcg gtgaagcgat tcagtacaga taagatgttt aactgtaagg taaacgtctc
     5821 tgcatatagc ccgtggcggc gtggagaagc tgaacttgct tatttgtgcc ttgatgcagt
     5881 gggcccggag gagctgaccc ggtttatcca gcaacgcaaa ttccggagta accacctttt
     5941 ttatattcgc ttttttaagg ccgcgatcaa attcctggaa caggagcgag aaaacgaact
     6001 gccgcaccgg caatatctgc tggctgcaat gcaggattcg gggatgcacc tgccggggaa
     6061 tatccaggaa ctgattcacc agtgtattgc ttcatggaag accggcaacc gtggggtttc
     6121 cctcgctgtc gggatgagta ccgaaaaagg gcgccaggca ctgctgaatc agctctacag
     6181 gctcactcag ggtgcaagcg aaatggtatc ggtcattcag gagcacgtcg ctgcgtccgg
     6241 ctcgcaactt cttcgggccg gggttaactc gtcagggaat tacgtcgcat ttatcgcgcc
     6301 taaaccctat gagtgtgaca acagactgga agcccatgcg tgggtccatc gtgttgtcta
     6361 tgcaaccggg aaacgaaaca ttcgggaaac cggcagatcc tgggtatcta tgccggagcg
     6421 ttcggcatcc gaaattactc tttgggaaga tgaggagcag tcacgaaaat ggtactccat
     6481 gacgcctgta ttcagttcgt ggaccgaaaa acagaagctt tttgagatgt gcgaaaaagg
     6541 cgcagggtta ctgaaagggg ctatggggac gccggatgat gaggaatatg gcgacctgct
     6601 agagatgtgg ggggacgcgt atatcgcgtg caatgccagc ggtgagcatg taacaacccc
     6661 ggatatgttc ctcccggtag gcctgattaa gtcacgaaaa tcattaaagc tgattgcttt
     6721 aggtacatgc tcgacggagc actggatgta tgcccgggca ggggatgatg actcaaggga
     6781 gctgctgctg gaattgtata ccagttggta tgagtaccca gataaagcgc gggcgcggct
     6841 cctgtcgatg gcagaaaaaa actccggcct gaggtttgtg ttgctcgaag gtaaatcaag
     6901 gaaactggag cgctttgcgg tcaagcggcc agaagagatt gtcaactggc atgccggtga
     6961 cttaaaggaa atcccgacaa tgctgaatga tcaatgggca tgtcacatgg caatggtcag
     7021 tcagaatgat aaggtctacc tgacaccgga tttgctcgat gatgatggtc tgcctaattt
     7081 taatggggtc accaggcagg tgcccggaga aggttatcag ccagtcaatg tctatgaatt
     7141 tgaaagtgac tattttaacg cggtatacga tgcagacggg aagaaagtgg cactttgtca
     7201 ctggtatgat gtgacggata attcatattc tgctgaagaa ctcataggca atatgccgca
     7261 taacgcattt aaatttcttc agtatagact ggataatatc gagcaggctg aggcatttat
     7321 caaatacaga aaccggaatt atcagccccg cgaaaatgca gactggcctg agccacctga
     7381 aggagttaag cgttacgtca tcagagcccg aagctaggtg aaaaatatta aggcaaaagt
     7441 tcagtatgga aattggtaaa tcatactgaa caattaatat gacattaggc atgactataa
     7501 gtaggtgcct tattggatgg tacatcgtat aaaaagtaac ttttctaatc tcacccagca
     7561 ctccgggaat gatgctaacc taaatcacgg gagcagtgct ggatattggt caatttatag
     7621 ttcctgtgga atgtaaatgt aattaaatgt tgcaataaaa cgtggttgtg ttaaaaggta
     7681 ggcttcatta cctaagttaa attttataat gtatgccaaa gaagaattta tactctgaag
     7741 agattgtgat tgccaaaaag caactcactc agctttcaac actaaaagat aaagtcagcc
     7801 tgctaacccg tcagtgggaa ggtgatattg gcgccaatct gcctggttac actgagctat
     7861 tagctcatgt ggaaagaatt gaaaggcaga ttcatgaaca aattggcagc tggaaaaaaa
     7921 ctcctgcaat gtaagattcg cctatccatt ctgaagcggt ggttttgttt aagggtaaaa
     7981 aaattcattc cttaatagtt ctgtgacgcc gcttttaaat cttggatgtt tgactctaaa
     8041 tgtctatcat ttaattaaca gtaattatga tgttgtcagc cttattcctg gctgaatctc
     8101 aatatattac catacgactt cattttgctt atcttcgggt tctcgaagaa ttccgccatg
     8161 attgtccata gcaggaggtt agtatgggtg tagacatgaa ttatgagttt cagaaaaaaa
     8221 gccctaaggg atgggaccgg gttaacgata atttttcaaa cgaccgaagc tatttgcttt
     8281 attcatggtt gggtctggat gcccgtaaca cctggggtgt tgcagcgatc actcctctca
     8341 ggggattacc cgatgacata gaacttcaat gggatgagga tggatgtgat gactactggg
     8401 gtgagcattc ccaaacgtgg ttactatccg acgagatcct tgcttcaacc agcccggtag
     8461 ccattgaaga cgatgaaccg gggtcggtag ttgcggagtt ttgtgcggag gttcagcgac
     8521 ttcatgggct gcatgggacg gtaagaattg ttctaggctt cactggttaa acaaggctgg
     8581 actatctaat gcctccagac aacgtaatcc tgacgtgttc atctactgta acctggtcgc
     8641 cggggatata tcgcggccac cctgacacgt catttgcatt tttgatgata aacgaagggt
     8701 agccgaactg ctcggccgca atatcgatat aaaaaatcat ttcaccggca cttacctcac
     8761 accagtaatg gccgtgcccg ccagactcat tgaaaatacc accatcatct gttccgttcc
     8821 cgcctcgcac agctgcggac atacctctgc ggtgcgcaaa ttcacaaacc agcagactgg
     8881 cgaacatgca tgttcctgtg gtgtcgcttg tcccattgtc attgaggata ttttttagtg
     8941 atgaataagc atcagttgcg agtgccagaa ctggagtatc aacgggaata gaggcctggt
     9001 cagtcatgaa agaatgtctc cggtatgatg gaacggctaa ttaaacaagc aatgagttga
     9061 ttccgttttt gagtacttca ctttcaggca accagtaccc ggaaccacac acccatgatt
     9121 cgcccgggaa aatgaaacct tcgcggacca ggaatactct aaaatttggc ggcagtacat
     9181 tttctatttt tagcatgtat ccttcctgaa tggatagatt tttcagctca gatagaagat
     9241 tcggtgagac gatgagctgt tcggtgtcaa gaagtccttg tagctcatgt aactcatcca
     9301 aggcctcctg aaggcgttcg gggatacaat tcagggaagc ctcgaaatgt tgctgctcag
     9361 cagcttcttc catccagtcc cgaaaccagc tgtattcctg agctgccgca tcccactcta
     9421 gtgtctcttc ttccgcttca ggatagttaa tagagatcca ttcatgacgc cattcttcct
     9481 gtatatcaaa cagcagttct ttaatgcttc ccataaacta tccccttgta tattttggag
     9541 ttcccaccgg actacattat acgatgattt gtggctgcaa atgttggtga cggttatcag
     9601 tatcgaccaa gcgccattaa ctggaagcac acttcctcgg aaagctctcc ttttttattt
     9661 gggatatgtg agggaacagc agttatcaca tcaacttcta cgcataaata ccatgtgtca
     9721 tcagcatcac tccaccaggc gacaatacga ttatcccagg tgcgtaattc gcggtagcag
     9781 ctgtatccag ttaaatgctc gaactccatc tcttgcatcc agactctgcc atggccaagg
     9841 atcatgagag cctcaaataa aacatccaat gaaatactaa tttccataat caacttcctt
     9901 attactacat ccatattatt tataatcact gtgcggggta atccattgtg ccacacagtg
     9961 attagtcaga taacagccac aaccacaatt gcctaacgca actttccact gtttcctaag
    10021 aacaagtaaa aacaaaattt acagaatcaa aagatgataa tttttgcgat ttgttccgtc
    10081 cggccggttt gatatacgtg ggaaggctgt tatttaatta catatattat atcacattta
    10141 tattttctgt cccaaaattc gcgaaaataa ttgtcacact ccgtctccag gatttttcgg
    10201 atctggaaaa atatgagggc aaaaaaacac aatctcacac gttgttttaa aagtatgcaa
    10261 gaaacgagag ggagatcaga aatcctaacg tattgattta tcagttagtg ggagtgtctt
    10321 atgaacttaa gagcaaaccc accagattta acataatcct agttatacgc actaagttat
    10381 tgttttataa agtgttttta ttttatggtg cgcacttgta tttacggcga gcaacagatt
    10441 t
//
LOCUS       DAMZII010000163        10335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZII010000163 DAMZII010000000
VERSION     DAMZII010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.163"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..690)
                     /locus_tag="POA77_004176"
     CDS             complement(1..690)
                     /locus_tag="POA77_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6367527.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(784..2931)
                     /gene="fdhF"
                     /locus_tag="POA77_004177"
     CDS             complement(784..2931)
                     /gene="fdhF"
                     /locus_tag="POA77_004177"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2512..2514),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI6367528.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(3129..4595)
                     /gene="mdtP"
                     /locus_tag="POA77_004178"
     CDS             complement(3129..4595)
                     /gene="mdtP"
                     /locus_tag="POA77_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDI6367529.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(4592..6643)
                     /gene="mdtO"
                     /locus_tag="POA77_004179"
     CDS             complement(4592..6643)
                     /gene="mdtO"
                     /locus_tag="POA77_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDI6367530.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(6643..7674)
                     /gene="mdtN"
                     /locus_tag="POA77_004180"
     CDS             complement(6643..7674)
                     /gene="mdtN"
                     /locus_tag="POA77_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDI6367531.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(7693..7968)
                     /locus_tag="POA77_004181"
     CDS             complement(7693..7968)
                     /locus_tag="POA77_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367532.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(8177..10162)
                     /gene="yjcS"
                     /locus_tag="POA77_004182"
     CDS             complement(8177..10162)
                     /gene="yjcS"
                     /locus_tag="POA77_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDI6367533.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2367 a         2738 c         2724 g         2506 t
ORIGIN      
        1 ttaaccgttg gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct
       61 caggttcaac cagtgcagcg ccttttgctt gttcttctcg ataaaacctt cttcaccgtt
      121 taagaacatc attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg
      181 tttgaaatac caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat
      241 caaaccaagc agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcgtt
      301 ttctaacagc gaaatcgcct ttggataatc cggtttaccc gcttgagtat ttaccagaat
      361 atgcgccagc gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac
      421 ttttgcttgt ggatagtcca gactgaccgg attggtgatt ttgatctgcg ccagcagcgc
      481 gcaggcatca gcatcaccgc tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa
      541 atcgccggag ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc
      601 tgctgccttt aaatactggc tgccaggctc ggagtcgttc gcgtgggcaa agaatgtcaa
      661 aaacaacatc aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc
      721 agtataaaga gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggact
      781 gtattacgcc agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac
      841 gtactgctcg gcggcgcgtt gatcggcgat cggctcgacg cgcacggcgc agtatttgta
      901 ttccggcgtt ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat
      961 ccaccactgg taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg
     1021 ggtgataatt ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatacccag
     1081 acgtttggcg tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag
     1141 tgccgcacag ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga
     1201 cagtaccatc gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa
     1261 gaactgtgcc agaccgttcg gggtatcaaa cttctcttta aacagataag aagtcccctg
     1321 atcggcatct gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttctc
     1381 gtaagtcgca ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt
     1441 gttgttgtag tgcatcggat aacccatacg ggtggcgatt tcactgatga tttgccagtc
     1501 cgttttcaga tcccatttcg gttcaactgc tttgaagaaa cgctggaagc cacggtcagc
     1561 cgcagtaaac acgccttcat gctcgcccca cgacgttgac ggcaaaatga catccgccgc
     1621 cgacgcggtt ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc
     1681 tttacgtact gccgacagct ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc
     1741 ggcacgcact tcgccatgcg ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg
     1801 agcaggcagg ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt
     1861 cacgtactgg tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac
     1921 gttgttctga ccacgaaccg ggtttacacc cgcatgcggc ttaccgaggt taccggtcag
     1981 catcgcgagg ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc
     2041 catgccccac aggatggcgg cgcttttcgc ctgggcatac atccgtgccg cctgacgaat
     2101 ctcactggcg ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat
     2161 tttacgatac tcttcaaagc cttctgtacg ggaagcgacg aacgctttgt cgtacagatt
     2221 ttcttcaata atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag
     2281 tgcaatgtgc atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat
     2341 tttcgccccg ttacgtttag cgttaattac gtgattcgcc acgattgggt gggaatccgc
     2401 cgggttgtac ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct
     2461 cattgcgcca ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg
     2521 agcgcagcag tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata
     2581 gttggtttcg ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta
     2641 cttctctttg atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac
     2701 aggttcgagt ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag
     2761 gatctgggta tcgttaatga aatcccagcc ataataaccc ttcagacaca gggtaccctg
     2821 gttggttttc ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt
     2881 gattttgcaa cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc
     2941 tccagttaat caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca
     3001 ttttatttgg ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa
     3061 acaggctgtc gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg
     3121 cagacatttt attttttctc gacgacggga gccgcctgat acccgccgcc cagcgatttc
     3181 atcaactgaa tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcttcg
     3241 gcaagtactg gcaaccgggc ttcagtggcc tgtaagcggc tggttaagcc gcgctgatag
     3301 gcggcctcgg cagcgcgctg ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct
     3361 cgctcgtcgt tgagcgtttg cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc
     3421 agtactgact ggttgtaacg ttcaatcatc atgttgctgg cggcgcgcgt gccttcgaga
     3481 ttggcattca accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac
     3541 tggcgactgg tttttttgaa taaggtatcc aggtggatgg catcaaggcc gaaaaacgct
     3601 ttgatatcaa agctcgggta gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc
     3661 tgaacatacc agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt
     3721 gtcgccggaa tgccggtctg gactcgtggt aatgccaccg gtttgatctc cggcatatcg
     3781 ctggcccccg cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgccctttg
     3841 acggcagcaa tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact
     3901 tgtgcttcca gaccgtgcgc cactttactc tggtgcgctt tcaccgcgta atcaatcaca
     3961 tcgcgagttt gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc
     4021 tgcgctacgc ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta
     4081 tgcgcgccaa tggcggcggc aaccgctgag cgatgcacac cccacaaatc aagatccagt
     4141 ccggcaaaca aacctactgt ggcttccgta tagtacggcc cgtccatacc cagtgcgggc
     4201 gcatccatcg cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc
     4261 cctaacgctg cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct
     4321 ttttcttccc gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc
     4381 agcgcatcca gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag
     4441 ctggcaagat gaatatcgtc ggccagtttg atttgttccg gtttgagctg ttgatgaggt
     4501 gcagaatcct tacgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg
     4561 cacaacagca gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg
     4621 tggcgggcgt taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt
     4681 gttgctcctg ttttaataag gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg
     4741 ttatttgagg agaacggctg agtgcggttg ccagaccggc agcgtatttc tccagggcgt
     4801 cggcaaactg agctgctctg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc
     4861 aggtggcatc ccaggcgtga agaatatcgc ggccctgacg aataaccgtt tgcgaacgtt
     4921 caatcagtaa ggcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca
     4981 tctcttcaca ggcattaaac gccgcatgca gaccgatacg aatttgcaga taagtgcgca
     5041 gagccgtgac ttcctgctgg cgtgggatcc gcattacttt acttaacata cccagcgtgc
     5101 cagccagttt ttgcggcagt gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca
     5161 ccgcggacac cacggtacca atgatgatac ccatggcgcg atcgcgaatt tccaccaggt
     5221 cgtacaccgg gccaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg
     5281 tgccgatata agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg
     5341 gtgccagcac aaacagcaat tcgacaatat tgtccagcca gggcatgacc agtagcgtga
     5401 ataacagcgc cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt
     5461 acgacgaacc gacatttggg ttagcgacga tcacgcatgt cagcatacag gtgtgaatgc
     5521 cttcccagtc cacgccgctg tagaaggtgt aacagatcaa acaggcgagc agtgttttta
     5581 ccgcgtagcg catatagtct ggattggtaa aagcatcggc gaccattgat ggcggtttgg
     5641 cggcgggcgt tggcggcgta ttcgggtcca tctggcccag ttgtaacaac gtctggcaga
     5701 tattctccag gttacattcc cgtgccgcca tcgcttcact ttcagtgatc cgccagtcgc
     5761 tttgccagca ctgaccttca gcaacggcat gctgcagctt attgatttct gaggctaatt
     5821 tttgccgaaa ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca
     5881 gcgtcgagta aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctttgagttc
     5941 gccagttggc atcgtccgcg agacaaaaga cattcagttt ttgcagcgcc agcgcctctc
     6001 tttcaatccg cgtttcgggt agcggtgcga ggctgtccgt caggtgacta atggcatcat
     6061 caagccgatc attaagcgct tgatgcattt gcgtaatggc acgactggga aaccacagca
     6121 cgccgattaa cgtcatcagc aaggttggat agaggccaac aacgatacac cacagcgtta
     6181 agcgcacgac cacttccgga tagtcgagca tggcggggaa ggtttgcccg taaatggcga
     6241 caatggcgac agcgaaaaag accagtccca gccgatgggt gcgcatcaaa aacatacagc
     6301 ccatcaggat cggcccggcg atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt
     6361 agatcaaaaa caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa
     6421 atttggtgta aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca
     6481 caaaagggat ctcaaaagtc atcgagatca gaatcaccag caggcagcct acccagagtt
     6541 gcacgatctg cggcacgcgg cctggtcgcc gctcgcttaa ctcttcatga aagaacgcca
     6601 gcagcctgac caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt
     6661 agcgactgcc gaagcgccga tgcggaacat ttccgggtca ggtttatcga ccatgatttt
     6721 gaccggaaaa cgctgggcaa cgcggaccca gttaatagag cgagaaactt tcggcaggcc
     6781 gcccagcacc aggccgccgt catccggtag cacgccgtag ccaatcgaat ccactttacc
     6841 ctcgaaggtt ttgccgctgt cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat
     6901 atttttcaga tcggtttcgc ggaagttggc gatcacgtac cagtgacgag tgtcgattag
     6961 ggtaaaaata gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac
     7021 ccggccatca aacggcgcgc gaacggtcgc catttccaga tgaagtttgg tcagggcaat
     7081 atccgcttcg accgccgcac gttgcgccac cagcgcatcg acgccgctga cggcgctggc
     7141 ggctgactgc gcctgtaaca atacggcatt aagatcggct tctgcggcgc gctgcgcagt
     7201 tcttgcacgg tcaacgtctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg
     7261 gcgtaatgta tctgtggcct gtttcgcggc ggcacgggct ttttctaccg tggcgttaac
     7321 cgagtcggca ccaaactgtt gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc
     7381 cagcgccgcg agggaggctt cagctttcgc cagattggct tcgtacgggc gcgggtcaat
     7441 gcggaacagc aaatcgccct gtttgactgc ctggttgtcg gtgaccgcca gttctacaat
     7501 gcgaccgctg acttccggca ccacatcaat ggtatctgct gacgcgtaag cgtcattagt
     7561 tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg ccaacgcaac
     7621 cactaacaga gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc
     7681 gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc
     7741 ggtataaata attccgacaa atgccagatt gatatttgcc cgctgaataa ctcgcctcgt
     7801 gatgagcatc aaaataaggc tggcaatggc gcagaaaaac cagctgggat aataagcacc
     7861 gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg gaattatcca
     7921 ggctgttttt ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc
     7981 ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc gtcagtatgg
     8041 ctattttctc gatgaataaa aatagcttta ttcagccacc gcattcataa cttatatatt
     8101 ttatttatta attggctgta tatattttta aaataaatgc ttttgggggc ttcagcgccc
     8161 ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg aaattatcca
     8221 gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct ttcttcgctt
     8281 ttaccagatc cgccattttg gcttgcccgg tcagcacggc gtgcagatct tcacggctga
     8341 tgtagaaaga ggcgtcagct tgggattgca gtgttttacg gtagttaagc acgctatcgt
     8401 tcagcgtcag gttgaggtta tcgccattgc tcatattgaa gttcaggctg atatttttac
     8461 ccgcggcttt cgcgctatcg aggcgaacgg acataaagtc gaacagcatt tcgaccgaca
     8521 tcccgcgaat ggtgtccggg gaaccggtgg tgccgtggct gaacttatgc accccttcgc
     8581 gcagctcttt cgcgccggtc aggtagaaac cgcgccaggt ggcggactcg gcctgatagc
     8641 ccagctgttc aaagttattc gcttgcagat tcttcgcgac ctggtcaccc gggttggcgg
     8701 caatcacctg tttcagcagt tctgccgacc agcggtaatc accttgcttg ttcgcttctt
     8761 gcgccaggtt gatgacacgg gcagaaccgc ccagcgcctg cacgtaacgt ttacccatct
     8821 ccacctgacc atacggatgc aggttagccg ggttaccgtc gtaatagcca agatagaagt
     8881 tatacaccgc gcgggcgttg tggctgacag aaccgtaata gccacggctg gcccagttat
     8941 tggcaagtgc aggcggcagt ttaatcatgt cgccgatttc attcatggtg tagccctggt
     9001 tcgccaggtg cagggtctgg tcgtgaatgt acttgatagt atcgcgatat ttaccaatat
     9061 aatcattgat atgcttattg ccccagaccg gccaggtgtg cggcataaac aggacttccg
     9121 cgtcgttacc ccacatatcc agcgtttcgt tcagatactc ggtccacttg ctggtgtcgc
     9181 gggttttcgc gccgcgcaga gtgtagaagt tgtgcagggt atgcgtggcg ttctcggcgg
     9241 tacacagcgc tttcagggcc ggaatataga agtgcatttc ggctggtgct tcgctacctg
     9301 gggtcatcag gaagtcaaac tccaggccgt cgataatcat cttctcaccg gttttggtga
     9361 tggttttgtt cggtgcgatg atgctcgggt agcctgatgc cagcgttacg ccaagaccat
     9421 tgccgatgtt accctgaggg ttatgcgcca gcagcaggcc gtaggagtaa agtgcacggc
     9481 ggctcataat attacccgcc agtacgtttt cgctgatggc ttcgtccata aagccagcag
     9541 gggcgataac ctgcacttta ccggatttca catcggcttc agagataatg cctttcacgc
     9601 cgccatagtg gtcggtatgg ctgtgggtat aaataaccgc aacaatcggt ttttgcgggc
     9661 gattttggaa gtaaaggtca agggcggctt ttgcgctcgg tggcgtcacc agcgggtcga
     9721 tgacaataat cccggtatca ccttcgataa aggtgatgtt ggagatatcc tgaccgcgaa
     9781 cctggtacat tctgtcggtg actttaaaca ggccggaaat accgtttaac tgcgactgac
     9841 gccacaggct agggttaacg gtttcgggcg cgggcgcatt gatatcaaat ttataatctt
     9901 ccccgcgata atatggtttg ccattagcat cgcgcagaat accttcatcc agcagcggag
     9961 cgataaatcc acgctgagcg tcgtcaaaat cctgacgatc gctaaacggt aatgatttgg
    10021 cgtaattctg attaacctgt tgggtatatt gagtggcggc tttcgcttca tctgttgcgt
    10081 aagcggtatt taccatcatg ccgctggcag cagttaacgc aataacaatc ctgctcaaac
    10141 ggaataaccg agagttattc atataaatct ccatttatca tttatgtcgt aaatatgtaa
    10201 tccatgcaaa atacgtcatt taaagctggg gttatcataa ttgggaattt tcgactttaa
    10261 taagtggaag tgtgagcgga acgatccatt ttattaggca tttgtgctga agggggaaaa
    10321 ttacgcgaat ttatt
//
LOCUS       DAMZII010000164        10301 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZII010000164 DAMZII010000000
VERSION     DAMZII010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.164"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..268
                     /locus_tag="POA77_004183"
     rRNA            <1..268
                     /locus_tag="POA77_004183"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            363..478
                     /gene="rrf"
                     /locus_tag="POA77_004184"
     rRNA            363..478
                     /gene="rrf"
                     /locus_tag="POA77_004184"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            493..568
                     /locus_tag="POA77_004185"
     tRNA            493..568
                     /locus_tag="POA77_004185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:526..528,aa:Thr,seq:ggt)
     gene            608..723
                     /gene="rrf"
                     /locus_tag="POA77_004186"
     rRNA            608..723
                     /gene="rrf"
                     /locus_tag="POA77_004186"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(786..1544)
                     /locus_tag="POA77_004187"
     CDS             complement(786..1544)
                     /locus_tag="POA77_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6367534.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1552..2655)
                     /locus_tag="POA77_004188"
                     /pseudo
     CDS             complement(1552..2655)
                     /locus_tag="POA77_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(2665..3846)
                     /locus_tag="POA77_004189"
     CDS             complement(2665..3846)
                     /locus_tag="POA77_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6367535.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3914..4939)
                     /locus_tag="POA77_004190"
     CDS             complement(3914..4939)
                     /locus_tag="POA77_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6367536.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5370..5591)
                     /locus_tag="POA77_004191"
     CDS             complement(5370..5591)
                     /locus_tag="POA77_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367537.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5844..8948)
                     /gene="acrF"
                     /locus_tag="POA77_004192"
     CDS             complement(5844..8948)
                     /gene="acrF"
                     /locus_tag="POA77_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDI6367538.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8960..10117)
                     /gene="acrE"
                     /locus_tag="POA77_004193"
     CDS             complement(8960..10117)
                     /gene="acrE"
                     /locus_tag="POA77_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDI6367539.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2726 a         2657 c         2505 g         2413 t
ORIGIN      
        1 tgaggggggc tgctcctagt acgagaggac cggagtggac gcatcactgg tgttcgggtt
       61 gtcatgccaa tggcactgcc cggtagctaa atgcggaaga gataagtgct gaaagcatct
      121 aagcacgaaa cttgccccga gatgagttct ccctgactcc ttgagagtcc tgaaggaacg
      181 ttgaagacga cgacgttgat aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg
      241 gtactaatga accgtgaggc ttaaccttac aacgccgaag atgttttggc ggatgagaga
      301 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
      361 tgcctggcgg ccttagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      421 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
      481 caaatttagc gtgctgatat ggctcagttg gtagagcgca cccttggtaa gggtgaggtc
      541 cccagttcga ctctgggtat cagcaccact ttttaggtta aagttcggca gattagaaaa
      601 gaatttgtct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
      661 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
      721 gacatcaaat caagcgaaag gccatccgaa aggatggcct ttttgctttt cgaactaaca
      781 ttcaattaat ggattacctg cgataaaaat gccctcgtac gctctgattt aggatgcgca
      841 aaaaattcat caggtgcagc ttgttccact atctccccac gatccataaa aattacccgg
      901 tcagcgacgg tacgtgcaaa ccccatctca tgtgttacac acaacattgt catacccgac
      961 tgcgccagcc caatcatcgt atccagcacc tctttcacca tctccggatc gagcgccgac
     1021 gttggctcat caaacaacat aattttcggc ttcatacaca gcgaacgcgc aatggcaacg
     1081 cgttgctgct gaccaccaga aatttgtccg ggaaacttat gcgcatgttc ggcaattctc
     1141 acccgctcaa ggtaatgcat cgccagagct tcagcctctt tctttggcat cttgcgtacc
     1201 caaatcggtg ccagggtaca gttctgtaga acggtcagat gagggaagag attgaaatgc
     1261 tgaaagacca ttcccacttc ctgccggacg cgctcaatat tgcggatatc ttcattaagt
     1321 tcgatgccat ctaccgcgat ccgtccctgt tgatgttctt ccagatgatt aatacaacga
     1381 atggttgtcg atttaccgga acctgaaggg ccacacagaa cgatccgttc tcccggttgc
     1441 acggttaaat ttatattttt caaaacatgg aattgtccat accatttatt gacgttttcc
     1501 agcgtaatca tcgcgttagc aggttgcagt aaaatttggc tcatagtgtc ctcaatgcgg
     1561 tgtacgcccg gtgttaaaac gtttttccag atgctggcta tagcgcgaca tgctgaaaca
     1621 gaagatccag tagatcagcg cggcgaaaac atacccttcc gtcgacatac ccaactaggt
     1681 gggatcaacg gttgcctgct gaacgctact gaaaagatcg aacaacccga taatgatcac
     1741 caggctggta tctttgaaga gtgcgatgat ggtatttacc agcccaggaa ttaccagctt
     1801 caacgcctgt ggcagaataa ccagcccctg agttttccag taacccaacg ccagcgactc
     1861 tgccgcttca tactgccctt taggcagtgc ctgtaatccg cctcgcacga cttccgcaac
     1921 atatgctgac tgaaacagga tcacgccaac cagcgcgcgg atcagtttgt cgatactggt
     1981 gccttctgcc ataaacaacg gcagcatgac cgaagacata aacaatacgg taatcagcgg
     2041 tacgccgcgc cagaactcga taaaaatgac cgataagata cgcacaatcg gcatatggga
     2101 gcggcgacct aacgccagta agatccccca cggtagcgcc cccgcaatcc caacggatgc
     2161 aataattaaa gttagcgtca gcccgcccca ttgccgggtt tcaacccgct ccagaccaaa
     2221 aaaaccgcca tacatcagcc accagacaat cagtgggtta atcaccgccc aggccgcaat
     2281 atagcgaccg cgatgcggga gtattttcca gaacattggt gcgatcgaca caagcccaat
     2341 cagtaatgcg aggttaattc gccagcgttg gtcgtgtggg taaagcccat acataaactg
     2401 accaaatcgt tcgtggatga agacccaaca ggcaccggct tttgtgcagt cggcacgcgt
     2461 tgagccaacc cagttagcct gcaaaaatgc ccagttcagc aacggtggga tcagttccca
     2521 catcaaccat atgcagccaa tagtcagcag gctattgctc cagctggaga acagattttt
     2581 tcgcacccac accatcgcgc ggcttgagtt atggctcgct gggcgcgagg gctgagacag
     2641 caatactttt gtcatcatga ctccttagcg ttcaacgatc gcgatgcggc ggttatagat
     2701 attcatcagc agcgagatag tcaggctgat aatcagatag accgacatgg tcatggctat
     2761 cgtctcgatg gcttgccccg tctggttcag cacggtgcca gcaaacagcg aaaccatatc
     2821 gggatagcca atagcggcgg caagagagga gtttttgacg atgttgagat actggctggt
     2881 taacggcgga ataatcactc gcagtgcctg gggaataatg acctggcgta gcgtaaccgg
     2941 gttgggtaat cccagtgacc gagccgcttc atgttgacca taaggcactg cctggatccc
     3001 agcgcggata atctcggcga taaatgcaga tgtataaacc gaaagtgcca gcgttaaggc
     3061 tgccagttca ggaattaaaa ccatcccgcc gcggaagtta aagcctcgta gggctgggac
     3121 atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt aaaggcaaac caatgatcaa
     3181 cactgcggcg atcggccagg tgcggcgcag ttgtccggtc tttatctggt atgttttatt
     3241 aaagcggaat agcccaacag aaaggactat agccataaca acagccagga taaacgcaat
     3301 aaatccgtct cccagctgcg gtgacggaat ataaagccca cgattgctca aaaaggccag
     3361 atcaaacgcg ctcacagcct ggcgtggtcc gggcaaattg cgcaacacgg caaagtacca
     3421 gaagaagatt tgcagcagcg ggggaatatt acggaagatc tcaatataaa ttgtggaaag
     3481 ctttcgtagc agccagttat ccgaaagtct cgccagaccg ataaagaagc ccaaaacaga
     3541 agcgaacaca atacacaatg cagaaaccag tagcgtattg agtaagccga caataaaaac
     3601 gcgtccgtag gtgtcgccct gctggtaatc gatcaaatgc tggacaatac cgaagccagc
     3661 gccgcgatcc agaaaggcaa aacctgaagt aatgccacga ttactgagat tcgttacagt
     3721 gttgtgaaat aaccaaccaa caatgccgac aacagcaaca acggcaagga tctggaataa
     3781 ccaggcacga accgtagggt tggcaaagga gagtgagcct ttaacggttg agcggcgatg
     3841 agacatagaa acctcggaaa gcataacgct gaagagagcg ccgccgtagc gacgctcgtt
     3901 acctcacacc gacttaacgc acgggcggcg cgtactgaat accgccgtta ttccagagat
     3961 tattttgccc acgtttaatt ttcagcgggc tttctgaacc tacattacgc tcgaaaattt
     4021 ccgagtagtt acccacctgt ttgatgatgt tataggccca tttattatcc aacttcagat
     4081 ccttgccgta atcgccttct ttacccagca gatgtgccat atcaggcgtt gctggattag
     4141 ccgctttttc atcgacgttc tgggaattga tgcccatctc ttcagcattc agcatggcga
     4201 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     4261 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     4321 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     4381 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     4441 ccttgaaata gtcggcaacg ttgagttcgg tatcagtacc cgcctgaata cagacggtag
     4501 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4561 cgtcgtaata agtgacgcca gtaaatgcca ttcccatccc ggcgtcgcga gatgaagtcc
     4621 aggtcgtatt acgggagagc aaatccactt cccctgactg taaagccgtg aagcgttctt
     4681 ttgctgtgag tggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4741 cacgacaaac atcaacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4801 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgaacg gcatcaagcg
     4861 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctagcggca gccagtgtgg
     4921 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4981 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccagcttt
     5041 tgcgcccgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     5101 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     5161 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggaag tgtgaaagtt
     5221 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaatcagcag
     5281 agaattagcc agcagaatat agcaatattt tatcttatgt aaagatactg tatcgttaaa
     5341 cagagcgcga aactccgcgc cctgttcagt tatttcgaca gagcgatgat ccccatcgtc
     5401 aggccagcaa cagccgctgc cccgccggca atagccccag ccgtttccca gttatcctga
     5461 gtcgtgcctt tcatacaggt attggtgtga accagacgac cctgatcgtc ataaaccgga
     5521 acgcagggcg aatcgtgagc acagcccgcc agcaatgcgg tgagcaatgc cacaggaatt
     5581 aatcttttca tggtgttacc tcagtaataa acgctaacgc ttaataagtt aactctctta
     5641 ttaacgagct gaatgccatt ttatcaccgg aggttataaa tattgcggaa tgaataatga
     5701 caaattatgt gaattgtaaa aatagtgaag ggagcgggaa ctatagaaag tttttaaaga
     5761 gtgagtggaa gaatatctta ttaattttta tgggtttcac tgaaaataat aaaggcaccc
     5821 gaaagtgcct ttatgtttct gatttatcct ttaaagcaac ggcggatcac cacaaagaac
     5881 accggtacga agaagattgc cagcaacgtt gcagagacca ttccgcccat taccccgata
     5941 cccactgcgt tctgcgcgcc actgccggca ccgttactga tagctagcgg taatacgccg
     6001 agaataaagg cgagagaggt catcaggata ggacgcagac gcatacgtac tgccattagt
     6061 gtcgcttcaa caacaccttt accctctttc tccatgagat ctttagcgaa ctcaacaatc
     6121 aaaatagcgt ttttggccga caagccaatt gtcgttagca agcccaccat aaagtagacg
     6181 tcattttttt gattaaagag tgtcgccgcc agcagcacgc cgacaatccc taacggcacg
     6241 accaacataa ccgagacagg aattgaccag ctttcataga gtgcagcaag gcacaggaaa
     6301 acaaccacaa aggaaattgc taccagagcg ggagcctggt ttcccgataa gcgttcctga
     6361 tacgacatac ccgtccagtc ataaccaatg ccagcaggta attttgacgc aaggttttcc
     6421 atcaacgcca tggcatcgcc ggaactggtt cctggcgcgg cttccccctg aatctccatt
     6481 gacggcagac cgttgtagcg ttccagtcgc ggagagccat acacccaatg tgaagtggta
     6541 aaggccgaga atggcaccat ttcgccgttg gcgctgcgga cataaagttt atcgacatct
     6601 tctggcagca tacggaattt ggcatccgcc tgaacataca cctttttcac gcgaccacgg
     6661 tcgatgaagt cgttaacgta agtcccaccc aacgccgttg aaatggtctg attgatgtca
     6721 gaaagtgaaa cacctaatgc ctgcgccttt tcctggtcaa cttccagttt aaactgcgcg
     6781 gtgtcttcca ggccattagg acgcacgctg actaagctgg caggatgttg cgccgccata
     6841 ccaagcaact ggttacgggc ctgggttagg gcatcgtgac ccagcccagc ctgatcaatt
     6901 aactcaaagt cgaaacccgt tgccgtgccc agttcaacaa tggctggcat attgaatgga
     6961 atgacaaaac cgtcgcggat cttgccaaat tccattttgg cacgatggat taccgcttcc
     7021 gcactgtttt catcaccatt acgctcttcc cacggtttca gactgacgaa ggccataccg
     7081 gcgttttgtg cctggccgct gaagctaaag ccgttaaccg taaagacact ttcaacgttc
     7141 gctttctcgt tcttcagata gtaatccgtc acttgatcca acactttttg cgtccgctct
     7201 tgcgtcgcgc cagcgggtaa ctgaatcatg gtcagaaaga caccctgatc ctcttcaggt
     7261 aagaaggaag acggaagacg taaaaacaac accaccattc ctgcaacaat cagcgcatag
     7321 atcagtaaat atcgtcctgt ggaaccgagg attttgccga cgctgttggt gtagtggtta
     7381 acgctatgat cgaaggtagt attaaaccag ccgaagaaac cgcccttatt ttcgtgatgc
     7441 tcagcagaga cgggtttaag cagcgttgca cataacgcag gggtaagaat caatgccacc
     7501 agaacagaaa gcgccattgc cgaaacgatg gtgatagaga actggcgata aattgcccca
     7561 gtagaaccgc cgaagaatgc catcggaata aataccgctg acagcaccat cgcgataccc
     7621 accagtgcgc cctggatttg cgacattgat ttttccgtcg cttcttttgg cgggagctta
     7681 tcctccatca tcacgcgctc gacgttctcc accaccacta tcgcatcatc gacgagcagc
     7741 ccgatggcaa gcaccatccc gaacatcgtt agtgtgttga tggagtaacc aaaagcggcg
     7801 aggatggcaa acgtccctaa caacaccacg ggtaccgcaa tggtggggat cagcgttgct
     7861 cgcatattct gcaagaacag atacatcacc aggaacacca gcataatggc ttcgaacagc
     7921 gtttttacca cttcgtgaat agaaagctgg acgaatggcg tggtgtcata agggtagaga
     7981 accttcattc cctgcgggaa gaatggctgt aattccgcca gttttgcctt aatggctttc
     8041 gcggtatcga gagcattcgc gccggttgcc agcttaatcc ccaggcccgc cgccggtttt
     8101 ccgttgatac gagcgataac gttatagttt tcaccgccaa gttcaacccg tgcgacatct
     8161 ttcaggcgta ccaccgagcc gtcactgttt acgcgcaggg tcactttgcc gaattcttcc
     8221 ggatttttta accgcgtttg agcaataatc gaggcattca actgttgccc tggtaacgct
     8281 ggcgttccgc ccaactgtcc ggcagcgatc tgatcgttct gtaccttcaa ctggttaatc
     8341 acatcaaccg gtgtcagttt atatttgttt agcagatcgg cgtccagcca gatacgcatc
     8401 gcatactgtg cgccgaaaag ctgtacgtca ccgacgccat tcagacggct aagcgtatct
     8461 ttaacgttag aggccacata gtccgagata tcgtcctgtg tggtgtctgg gttatcagag
     8521 acaaagcccg ccaccatcaa atagctgcta ctggactttt caacactgat cccctgctgc
     8581 tgaacctcct gcggcagcaa cggcgtggcg agctggagtt tgttctgcac ctgcacttgc
     8641 gcgatatcag gatcggtccc ggactggaag gtaagggtaa ttgtcacgct accggcggaa
     8701 tcgctggtgg aggacatata catcaggtta tcgataccat tcatattctg ttcgataacc
     8761 tgcgtcaccg tatcctgcac ggtctgcgca tcagcacctg gatagtttgc cgaaacagaa
     8821 accgcaggcg gtgcaatggt tggatactga gcgacgggca attgtaggat cgccagtgcg
     8881 cccgccatca tcagaataat ggccagcacc catgcaaata tcggtcgtcg aataaaaaag
     8941 tttgccatgt cagattacct tacttcgatg cagtatctgc gggggtatcg gtagtggctt
     9001 taacctgctc tcccggacgc gctttttgca ggccgctgac aatgacttga tcgccagatt
     9061 tcagaccttc gctaatcaac catttatcgc caatcgcctg actggcaacg acagggcgcg
     9121 cttccacctg acttttatcg ttaacaatca gcacggttgc atcaccacgc ggtgtgcggc
     9181 taacgccttg ttgcgggata agaatggcgt caggttggac gccttcatca atccgtgcgc
     9241 gcacaaacat acccggcaaa agcgtatgtt gcgggttagg gaagacggca cgtagggtta
     9301 tggagccagt gctttcatca acggtcacat cagagaattg cagcgtacct ttcaggggat
     9361 aggtttgacc gttttccatg accaactcta cgttgctggt ggcgttttcc ttatgcaaat
     9421 ttccttgctc tacggattgc ttcagcctca taaaatcgtt gctggattgg gtcacatcaa
     9481 cgtagatagg atcgagctgc tggacagtcg ccagttcagt cgtttgccca ttagtgacaa
     9541 gagcgccttc ggtcacggtc gatttgccga tacgtccgct aattggcgca gtcactttgg
     9601 tataagcgag attgatgcga gcgctttcga ctgtggcttt tgcggcaatc acggcggcat
     9661 cggcctggcg agcatcagca atggcctggt cgtactcctg ctggctgatg tatttcgtac
     9721 ccacgagcgg aacgtaacgt tttaccgtca aatgcgcgat ggcggcggcg gcttcacttt
     9781 tcgccagttc gcctttcgcg ctgtcataat ttgcctgata ggtcgcggga tcgatctggt
     9841 acagggactg gcctgcttgc acatcgctgc cttcagtgaa attgcgattc agtacgatcc
     9901 cgctaacctg tgggcgaact tcggctatac ggtaagcatt ggtgcggcct ggtaattcag
     9961 tcttaacttc taacggggcc gtttttacaa tatgaacggt aacctgcggt tcaccgacgt
    10021 gagctttctc ttctccctta tcgttacaac cggcgattaa agccgcggag atcagaataa
    10081 aggagggcag gaggaaaaac ctggcatgtt tcgtcattac tattcctcaa aaaaccaaaa
    10141 gcgcgttatt tacccaaaaa ggcaacgcgt taactcgcag aaagaaaaat acagttcgct
    10201 atcctacaaa ttatcattcg tcgatgtaag gaatagttat gaatacaggc gtctcaaggc
    10261 acataaacac aaaaaaagat taatattcta ctgttttatt t
//
LOCUS       DAMZII010000165        10290 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZII010000165 DAMZII010000000
VERSION     DAMZII010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.165"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..411)
                     /locus_tag="POA77_004194"
     CDS             complement(<1..411)
                     /locus_tag="POA77_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDI6367540.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGE"
     gene            complement(458..760)
                     /locus_tag="POA77_004195"
     CDS             complement(458..760)
                     /locus_tag="POA77_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6367541.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(1301..2077)
                     /locus_tag="POA77_004196"
     CDS             complement(1301..2077)
                     /locus_tag="POA77_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367542.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLAWDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(2123..2557)
                     /locus_tag="POA77_004197"
     CDS             complement(2123..2557)
                     /locus_tag="POA77_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDI6367543.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            complement(2571..2792)
                     /locus_tag="POA77_004198"
     CDS             complement(2571..2792)
                     /locus_tag="POA77_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367544.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(2793..3476)
                     /locus_tag="POA77_004199"
     CDS             complement(2793..3476)
                     /locus_tag="POA77_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDI6367545.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3552..3864)
                     /locus_tag="POA77_004200"
                     /pseudo
     CDS             complement(3552..3864)
                     /locus_tag="POA77_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3861..4763)
                     /locus_tag="POA77_004201"
     CDS             complement(3861..4763)
                     /locus_tag="POA77_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDI6367546.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(5444..5836)
                     /locus_tag="POA77_004202"
     CDS             complement(5444..5836)
                     /locus_tag="POA77_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDI6367547.1"
                     /translation="MYEGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(5840..6814)
                     /locus_tag="POA77_004203"
     CDS             complement(5840..6814)
                     /locus_tag="POA77_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HDI6367548.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRNNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            complement(7053..7427)
                     /locus_tag="POA77_004204"
     CDS             complement(7053..7427)
                     /locus_tag="POA77_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367549.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            complement(7427..8059)
                     /locus_tag="POA77_004205"
     CDS             complement(7427..8059)
                     /locus_tag="POA77_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI6367550.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            complement(8643..9428)
                     /locus_tag="POA77_004206"
     CDS             complement(8643..9428)
                     /locus_tag="POA77_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDI6367551.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
     gene            complement(9430..9843)
                     /locus_tag="POA77_004207"
     CDS             complement(9430..9843)
                     /locus_tag="POA77_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367552.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
BASE COUNT         2408 a         2944 c         2587 g         2351 t
ORIGIN      
        1 ctccccgtcc agcgcctgta acacaacgtc cattgcttcg cgttcttccc tgacgaacgc
       61 atcaccgtcg cccccgttct cactcccgac ggcatacagg cgcttcattt cactgcctgc
      121 cagcgccaac actttttcca gcatcaccgc cgggactgtc acctgacggc tggcgcgctt
      181 ttcttccatc agttcacgaa caacgccgtg aacaacgctg acatccgcac catattcaaa
      241 ggcatcgtcc agccgctgca tgacggtttc cagctcatcg tctgtcagac tccagtcctg
      301 ggctatgtgg cgcacatccc cacgggtcag caccacggca aaaacgcatt cactgtccgg
      361 cacgtctgta tccagtacat cgcggataat ttctttaaca gtacagtaca tgttctcgtc
      421 tcctttacag gggcggcatt gccgccccgt ccgttaatca gtccagtgct gcgtaaatcg
      481 cgttgcattc agggtgaaat tcgatgtgac gccacaactg cgcatcgcgc atccggtaaa
      541 gctgggtcag tcctgcatcc ccgctgtcgt ggtacagcca caactggcgg ttaatcacaa
      601 gggaggtaag gatgatgcct gcggcatcag cactcacggt acggtcaaac cagtttgcac
      661 cgttcaccat gtgaaaacgg tcgccatccg gcatcatgta accgccaccg tccggcagtc
      721 ggatgaagtg ccagaatccg ccttcatatt ccggcatcat ttgtttcgcc agcgcgtata
      781 cagacgcttc ggcatacaga aaatcctggc caaacaggta aggcagaaaa gacagacggt
      841 cgcactcttc aacgttcaga gtgacaggtt tcgcatattg catgatgttt tcctccgttg
      901 ttcacatatt ccgcgacacc gggcggtgcc gtgcctccat aaaagccggg cgataaggtg
      961 gggatgcaca gggcgcaagg gaagccgcag cctgacgcac acggctggcg ggagttggag
     1021 ggtgagaccg cgaaatctga gcgggtgaac gaccggaaaa tcacggcaga cgggaaggag
     1081 ggaccgggtg accgcagcgg cgaagcgtac ccttgcatcc ctgccagccc ggctaagatg
     1141 gaggcacggc acagcccgca cacgacacgg cggccacata actgaaaaac cacaaggcca
     1201 tgccctgcat ggccgggtaa agcggagccg gcggcgacgt cctttgtgct ctgtccgggg
     1261 accggcagga aaaaggcggg gatttacccc gccagtgtca tcatttccgg atttttgcca
     1321 gggcatccgc catgctgcgg atttcgttcc gggcttcccg gaccacctcc cgcagaacac
     1381 cacggtcaaa ccagcggcgg ggcggttcat ccggtatacg cagcacatcc caggccagcc
     1441 aactggacag gatatgttca atacaatcgc tgaccagata catttccacc gccgaaaaac
     1501 gtgcgccccg gttcaggatg gcgtcgagca ccacgggcag gttttcgtcg tcctcatcga
     1561 gccagcaggg gtgatcgctc tccttcacga ccaggcgaaa gcggcgcgtc tggcgggtca
     1621 gtaacgtctg accggtggtg gtcagcgccg cccggatacg ccgcgtatct tctgccacct
     1681 ggtaactgac acagcgtact gcatcgcgta ccgcatcaag aactgccctg atttcgtcct
     1741 ctgccagcca ctcaccggca tacgggaaag acacctgcgt tccgtctgcc ggaagtaccg
     1801 gcagcgactg ccactgactg ccggacagta caagttccgc cccggttccg gtcagtttgt
     1861 gaatggcgga ggccaggagc ggcggcagtg cgccgccgtg acggttgata agtaacgtcg
     1921 ccagccgggg cgcatccagc agaacataaa tgctgatcca gcccggatgg ttcgggttcc
     1981 gctggtcaac atccgtctct tccggccagc cctgcgccag cagcaggttg cgggcgtgac
     2041 gacaaatcag tgcgttcagt gttgtgtttt cgttcatgat taatcgttct ccctgaatcg
     2101 ccgcccggag gcggcgataa catcagtcgt tttcgtgtct gttgtttttc agcagggtgc
     2161 gggcctgatt aatcaccgcc tgacggcggg tgacgcattc cgggacggcg aggccatgat
     2221 cccgggcggc ccattcacgt ttccacaatg cctgttcagc ggtctgaatc agggacgcca
     2281 gcagttcagc agggacggtc acctggcggt tttcgctcac gttattgatg atctccatcg
     2341 ccacgccgga agaaataccg gattcagtcc gctggtcttc cgggatgtcc tccaggcgcg
     2401 ccaggacggt tctgatttca tgatcggaca gggaaatatc cattccgtca gcgagggcct
     2461 gaatctcttc cggggaccag ataaccagca tcagcggcat gtctcccgga tactttactg
     2521 ccagctcccg gcatagcgtt tcgcatgttc cgtacattct cgtctccttc tcaatcttta
     2581 aagcacgctt ccagttccag aatttcggta tatgcctcca tcaggaggcg gtttgcgcga
     2641 aacagcgtct gacgcaccag acgttccggc agggtgtcgg cattaaagct gtaccgctgc
     2701 tccatctcat tcatggttgt acgcaggtcg cacatggcat tggccacctc cgccacttcg
     2761 gcgcgcagtt tttcgtgtcc tgcatagttc atttacgcag cctccggcat aaaccagtca
     2821 tcattcgcgg ccccctgctg catggcccgt tgcacggcgg caaggcgttg ctgcccggca
     2881 cggtgatact gctcaagcag ctcgataccg atataccggc gtccggactg gagggcggcg
     2941 acgcaggttg agccgctgcc tgcaaacggg tccagcacaa ttgcgttcgg gtgtgtgaag
     3001 ttctcaatca gcggttgcag gctggtaacg ggcttttccg tcgggtgatg gcgattgccc
     3061 gaatatttcc agcccagcac gtccggcagc gggttttgtg gcagacgtgg acggcctttt
     3121 gccaggatgt aggcgcattc gtggcgatag cccacatatg cggccttcga tgtgtagttt
     3181 ttggtgaaga ccaggtgacc aacaacgctg aatcccgcat ttttccaggc ggccataaag
     3241 cgatcgacgc ggttccagcc gtagaagctc accattaacg cgtctttttt cagtacgcgg
     3301 aacatttcat tacaggccgg ttgcagccac tcatcggttt tatcgccggc gatggtgcgc
     3361 ccggaacggt cacggaaacc gacgagatat ggcgggtcgg tgaggatgaa atcaatggcg
     3421 ttatcaggga tacgggccat aacatcgatg cagttaccga ggacaaaacg ggacatacca
     3481 aactccttct cttacagagg gcaccgggag ggcacccctg tggtgcccgt tccccgctgc
     3541 ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt
     3601 cgtctttctg acggcaaggt tatgccgcga aagcggcttg aagggcgttc atcagaaaag
     3661 ggcgacaggc tggcgtacac tgtcaccgac gccagccggg agcggtaaaa gcgcccgtcc
     3721 cgtgcccggc cggacgcggg acactgacca caggcagcgt ttatgccgcg ccgcgtcagc
     3781 cggcgcagta aaaagaggag cacggagaca tcctttaccg gaaaagaaac gggcggtagc
     3841 ccgcccgtct ccgtcatact tcatccgcca taatgcgcca ccttgtcgac tatccacgca
     3901 ggtccggtga cttccggcag ctcatcgtca tctgtcgggg aagaccattc cagctccccc
     3961 cacagcacat caacgagctc tccgcgctca tacaactgcc agccacagaa atcacagccc
     4021 tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc tgcaataacg
     4081 tcgctttccg gctgacacca cggcgtgtcg aaatcaacct gaatgaagtt gtctccctga
     4141 ctgctgatat tcaggtcata cccacaaggc cacttcacgc catactgttc ggtataccag
     4201 tgatatgcag aaggaacacc gttcagaaca ccaccgttaa agccattcac ttcaacatca
     4261 aggcgggtgg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg cagtacgttg
     4321 tcacacagac ggttccacca caccgataca tcctcgttac tccagaagcc acaccagtca
     4381 cctctttttg cggtgaaaag cgcggtgatg gtatccctga catcgtccgg taatccttcc
     4441 cagcgacgct ggccagtacc gctctgcagc cagagttcat gcagcatcag gcagttttgc
     4501 tcatccagca gtacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc gatattctcc
     4561 ggcgatacaa cgccacgtcc ggcagccgtc agtccggggc acggttcaaa ccacacatct
     4621 tcagtggtct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc ttcatttgtg
     4681 gcgcggcgat aaaacggtgt gacggcacca ctggccagtc gtttaatctc agcaatctgt
     4741 gccggttcac cagaaaaatg catacggtta ctgcaccagt ttggcatggg aattctcctc
     4801 tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc
     4861 aggtgcggcc ccgaagccgc agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg
     4921 aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc
     4981 tgggtaacca ggtattttgt ccacagaacc gtgcacaaaa tgctgtggat aagctggaca
     5041 cagcactcca cagcaggcat acagccatac cctcagagaa ttgagcgttt tttagtcaga
     5101 acaatgacat ttgactgtgt gcggattcag ttattagaat actgtatata tatacagcaa
     5161 aagaaaagga agatgagaac atgatccgca ttgaaattct tttcgaccgc cagagcacaa
     5221 aaaaactcaa atcaggcaca cttcaggcgc tacagaatga aatcgaacaa cgtctgaaac
     5281 cacactaccc ggaaatctgg ctgcatatgt gggaatcccc gtccttcaga gtgaggagct
     5341 gtcaaccagc actccattga ctcaaatgac gggaaacgcg gaaatctaaa aattaagccg
     5401 cacaccccga gaaattttgc aggtttcacg gagcgagact tttttagatc aggttttgca
     5461 tcttcaccct agccgctttc agacgggcct gttcaccgtc atcctgagag tgttctggtg
     5521 aaactgtact ctgaagccct cccaacgcct tcagtacctc ttttaaatca ccggaggagg
     5581 gtttccagcc tgttatcctg gcaatctgac taatgacctc atccgcattc aattctctgg
     5641 tgaatagcgc ggttaacacg gcgggaattc gctcatcaag ctgatgcatt atcaggccgg
     5701 ttatcaaagc ctgccgatat aactcccccc gttttttccg gggaacagtt tcgatttcag
     5761 ccagtgcaag acaatcggca gagtcctccg ggtgaaggta gagtgaaagt tttttccttt
     5821 cttcgccttc gtacatcgat tactcctccg ctttgaagcg tgcaatagcc tggaccagcg
     5881 ctgtctgagg ttcatccata agaaccacct tttcgccaag gtggttccat gccttacgaa
     5941 cagcatcagc aatgagtgca gccccgccac cgacaatata tacgcggttt acgtttcgat
     6001 actgcagcaa atcatcaaca actagctcgc ccagtttatt tatcgctgta tcaatggtat
     6061 ccagcaccag atttagttta gagacctcat ttacaacctg ccccacaaaa tccaggttat
     6121 tcctgttttt gatcaactcg tcagcaatca ttggactggt atcactgctg gccattttca
     6181 gtgcagtgag cgtggctttc gtgaccattg atacaccaat atcaggatta ccatgtacag
     6241 ccgaaacatc ctcaaactgg ccaacaataa ccccgacatc cagagttgta ccaccgagat
     6301 cgataaccaa tgatttttcg tactgaccaa cgttgtcagc aactaggcga gtaaatacag
     6361 ccgggagtga ttcaggcatc acttcaacgc ttttaatggt aaaagttccc cctttgttca
     6421 gggtgacttc tcgcatcagg ttgcgaattt ttctctcaat gttaagcgta tttttctggc
     6481 agtcggccgt gtaaaactcg ctgataggta acgtaacggt gagatcgatc tcctggggtt
     6541 caatgccact gttcagtaat gcgtggtgaa ctgccaacac atttgtatca gagtactgat
     6601 actcaatatg agtcgtgctg atcgcatctt cgctgaccgg gtcgtaggta tatttacggc
     6661 catcaagcag atagttataa gtacgactgc tgcctaagcc ctcgactttc cagttatggc
     6721 gaaaagagtt cactgatacc gcacttttca gcgtcttgcc ttcaaaccag gcaagtttca
     6781 catttgtaga accatcatcg caaaaaattc tcataccatc tccatttata ctcaacttga
     6841 gtattcatct ttacacaata actactcaca ttaagtaaat acgctcataa accacaggta
     6901 agtactcaag ctgcgtactt cacttgggca gttaactact caatttgagc attatatcat
     6961 ttatcttgca gcaatctagc atttatctag cataaatctt gctgcaagat tgcacacaaa
     7021 aaaagaatgc agcaagattg ctgcatcccg gattaatagc cctgtttttc cttatatgcc
     7081 tcaactgctt tttccagaac ttccaccagt ttcaacccct cacgctctgc gatatcccgg
     7141 atctcttggt cgaaagcagg tgtgacacgt gttgcaaatg ccagcgtcct gtttgttttt
     7201 ctggcagaac ggccatcgcg gcgggtatat acctcggtca ctgtttgcgt aaccacaggc
     7261 tcatggtgca ctggcgatga gtctgcctgt tgtctctgaa catcagtggc agcgacagcc
     7321 ggagctatct caggcgcatt caagctggta gccacttcgt cggggcccgg gggtaccccc
     7381 aacccactac gggttctttt agttctaagt tttgacgcat cagccattac tcttctccaa
     7441 tcttatcgat gagtgcctgg ataagttcat cagctctctc gtttaaacct ttataacgtg
     7501 tttctgtcac agcgagtcct gaattcatcg cttttctgta tgctggcttc tcaaaaaggc
     7561 atccagataa agtttcataa cctgcttcac tgatatatgc acgagcatcc agctcttccg
     7621 cttcggtacc tacccggctt aaagcgaaga ccagttttga acgtggtata ccttctttta
     7681 ccaacgcgtt gaaaacttta attgccggga caagatcgtc aagggaggct cccgttggct
     7741 ggacgataag gtcggcgacc ttagcaattt ctgaagtacc tttacttgca cgagcagggc
     7801 catcaagaat cagaaggtca aaatcgccgg cagattctat cgcctgcttt gctgttgaaa
     7861 acacttcaac ggaagcaacc ggtgacaaac cagctgacag acgtctacga tgccactcag
     7921 cactcgttgc ctgttccaag tcgagatcag ctaattttgt tttgatacca caggcggatg
     7981 cttcacgcgc taacgctctc gctttagtgc ttttgcccac accacctttc tgagaaataa
     8041 aaccaactat ccaggccatt tagccccctt tacttgctgc tatcttgcag caaacatcaa
     8101 tataaaccag attgagatta ggtagagtaa ctcttccaga acaagataac agcttgcagc
     8161 aagattgcaa caataaataa tcttgctact tagcagagtg ctggaattga cagccgaata
     8221 cttgtgtcac gttaacaata aaagaaacct cattcagaat cgcatcgaca gatgaagcaa
     8281 gattgccgca agattgcaga tagcataaca taaacagagc gctaggccgt atgtgtacag
     8341 catgatagct gcaatctatt ttattgctgc tatcatgctg aaatatcatt attagaatca
     8401 ggcagccctc gccgcagcca tgtaactgtg cgtagcgagc gaactggcga ggaagcgttc
     8461 cggcagatgg ctcttacgct cggcacaaga agagtttccc atccaggcaa gtactatggg
     8521 ttgtcagcgc cagagatata agactgttct ggcctgagaa cgtggcctct atgagtttgg
     8581 tgctctatct tccatagatg aatgcaccag ctcccccttg actgaaccgc gaatacggct
     8641 ttttaacgcc tctcaagttg ttttatcagc gctaccgcat cggcttccgg catggcgaac
     8701 tgcacacgat gacgcgccgc gacatccagc gcaaagacct tcgtgtagac ctccgttgag
     8761 ctgatggact tatgccccat cagactctgc aggaccttca gcggtatacc ggcatacagc
     8821 atgtgcatcg cataggaatg gcggaacgta tgtggcgtga ccgggacaga gaacgtcact
     8881 ccatcagcgg cagcggcggc aaccgcctcc ccaatccagg tcctgaccgt tctgtccgtc
     8941 acttcccaga tccgcgcttt ctctgtcctg cctgttcgtt tattacgccg ttccatgggg
     9001 attttcagtg tggccaccat cgtctgcagc tggctgacgt accaggcgtc agagagcgga
     9061 accagccggt gagtctgctg accggcgggc attcttcctg ccgtcctggc ggctttttcg
     9121 gtccgctgct tcagagtggc caactgcaca aacggatacg gaggcgcaag cgaaaaatcc
     9181 ccccgcgtca gcgccagtgc ttcattaatg cgcgcaccgg tgttccacag tgtggccagc
     9241 agcattttgc ggcgcagatc cggcacgtaa tggagcaggg cgctcacttc aggggccagc
     9301 aggtattttg gcagttcatc atgaaccatc gacatctggc ggagtgccag cgctgccgga
     9361 taatcgatgg caacaggaag ctgagcagca tcccgggcat ggtggatcgg tgccggcagg
     9421 tgctgcatat cagtgttcac tccacgggga aacgtcgggc aggttttcca caacacggta
     9481 ctttcccgga taacggagct ggttacctcc acgcgtccag acggtgacaa caaaaccccc
     9541 tccctggttg ttttcgggga gcaggcgatc gcactgctca ctcagcatgt ggatcacaaa
     9601 cggcgcatcg ctgccgtctt caaataccag ttccagcgca tcgcggccat gcagtgctcc
     9661 cttactgatg atgacgtact cagccccccg catctccctg agtaaatgtt ttgccgcatc
     9721 cgggacaagc agcctggctg cgccggcatt ccagctgagg tagacatatc cggcctgcgc
     9781 ctgtacagac tgccagtaat ccgttccggc gacagcctga ccttcattct caataaaaaa
     9841 catgggtgac tccttttttc ttttccgggc agggacggtc atgcgataac gtcatccatc
     9901 atcactgccc ttaccgtcac gccgtctgat aacatcatgc cgttatcatc atcaccatga
     9961 taacaagcat ccatataacc acgccgttaa attaccggag agccgtttcc gtcagatccg
    10021 actttccgga atatcacaac tttccggaag tgtaccacgc tgagaaaatc atcatatcgc
    10081 tgttttccgc ccctgcgcgg cgcatcacag gtcagggaga tgacacaaca tatagtgtca
    10141 tcagcagctg aattcagcac tacatgttgt gctgacggga gatggcgctg tgcggaggga
    10201 gaggaagatc cgtaacagcc ggaaaccgga tctgtgcggc agagaacggg caggagatat
    10261 gataattaag cagcacacat aaaatcatat
//
LOCUS       DAMZII010000166        10148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZII010000166 DAMZII010000000
VERSION     DAMZII010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.166"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            97..1491
                     /gene="galP"
                     /locus_tag="POA77_004208"
     CDS             97..1491
                     /gene="galP"
                     /locus_tag="POA77_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDI6367553.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1568..2065
                     /locus_tag="POA77_004209"
     CDS             1568..2065
                     /locus_tag="POA77_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDI6367554.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2160..2867
                     /gene="endA"
                     /locus_tag="POA77_004210"
     CDS             2160..2867
                     /gene="endA"
                     /locus_tag="POA77_004210"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDI6367555.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2947..3678
                     /gene="rsmE"
                     /locus_tag="POA77_004211"
     CDS             2947..3678
                     /gene="rsmE"
                     /locus_tag="POA77_004211"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI6367556.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3691..4641
                     /gene="gshB"
                     /locus_tag="POA77_004212"
     CDS             3691..4641
                     /gene="gshB"
                     /locus_tag="POA77_004212"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDI6367557.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4750..5313
                     /locus_tag="POA77_004213"
     CDS             4750..5313
                     /locus_tag="POA77_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDI6367558.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5313..5729
                     /gene="ruvX"
                     /locus_tag="POA77_004214"
     CDS             5313..5729
                     /gene="ruvX"
                     /locus_tag="POA77_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI6367559.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5905..6885)
                     /locus_tag="POA77_004215"
     CDS             complement(5905..6885)
                     /locus_tag="POA77_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDI6367560.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6903..7607
                     /gene="yggS"
                     /locus_tag="POA77_004216"
     CDS             6903..7607
                     /gene="yggS"
                     /locus_tag="POA77_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDI6367561.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7625..8191
                     /gene="yggT"
                     /locus_tag="POA77_004217"
     CDS             7625..8191
                     /gene="yggT"
                     /locus_tag="POA77_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDI6367562.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8188..8478
                     /locus_tag="POA77_004218"
     CDS             8188..8478
                     /locus_tag="POA77_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDI6367563.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8486..9079
                     /locus_tag="POA77_004219"
     CDS             8486..9079
                     /locus_tag="POA77_004219"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDI6367564.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHTAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9072..>10148
                     /gene="hemW"
                     /locus_tag="POA77_004220"
     CDS             9072..>10148
                     /gene="hemW"
                     /locus_tag="POA77_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDI6367565.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     NIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQ"
BASE COUNT         2381 a         2684 c         2695 g         2388 t
ORIGIN      
        1 gtaagcgatt acactgatgt gatttgcttc acatcttttt acgtcgtact cacctatctt
       61 aattcacaat aaaaaataac catattggag ggcatcatgc ctgacgctaa aaaacagggg
      121 cggtcaaaca aggcaatgac gtttttcgtc tgcttccttg ccgctctggc gggattactc
      181 tttggcctgg atatcggtgt aattgctggc gcactgccgt ttattgcaga tgaattccag
      241 attacttcgc acacgcaaga atgggtcgta agctccatga tgttcggtgc ggcagtcggt
      301 gcggtgggca gcggctggct ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc
      361 gcaattttgt ttgttgccgg ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg
      421 attctttccc gcgttctact ggggctggcg gtgggtgtgg cctcttatac cgcaccgctg
      481 tacctctctg aaattgcgcc ggaaaaaatt cgtggcagta tgatctcgat gtatcagttg
      541 atgatcacta tcgggatcct cggtgcttat ctttctgata ccgccttcag ctacaccggt
      601 gcatggcgct ggatgctggg tgtgattatc atcccggcaa ttttgctgct gattggtgtc
      661 ttcttcctgc cagacagccc acgttggttt gccgccaaac gccgttttgt tgatgccgaa
      721 cgcgtgctgc tacgcctgcg tgacaccagc gcggaagcga aacgcgaact ggatgaaatc
      781 cgtgaaagtt tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa cagcaacttc
      841 cgccgcgcgg tgttccttgg cgtactgttg caggtaatgc agcaattcac cgggatgaac
      901 gtcatcatgt attacgcgcc gaaaatcttc gaactggcgg gttataccaa cactaccgag
      961 caaatgtggg ggaccgtgat tgtcggcctg accaacgtac ttgccacctt tatcgcaatc
     1021 ggccttgttg accgctgggg acgtaaacca acgctaacgc tgggcttcct ggtgatggct
     1081 gctggcatgg gcgtactcgg tacaatgatg catatcggta ttcactctcc gtcagcgcag
     1141 tatttcgcca tcgccatgct gctgatgttt attgtcggtt ttgccatgag tgccggtccg
     1201 ctgatttggg tactgtgctc cgaaattcag ccgctgaaag gccgcgattt tggcatcacc
     1261 tgctccaccg ccaccaactg gattgccaac atgatcgttg gcgcaacgtt cctgaccatg
     1321 ctcaacacgc tgggtaacgc caacaccttc tgggtgtatg cggctctgaa cgtactgttt
     1381 atcctgctga cattgtggct agtaccggaa accaaacacg tttcgctgga acatattgaa
     1441 cgtaatctga tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta atctcctcag
     1501 gcttcctccc gtcgcggagg aagccacctc ttgcagtcat cttttcttcg ctctatcctc
     1561 tgccgctatg aaaacatccc gtctccctat cgccatccaa caggccgtta tgcgccgcct
     1621 gcgggaaaaa ctcgcccagg ccaacctgaa gctagggcgt aactacccgg agccaaaact
     1681 ctcttacacc cagcgcggaa cctccgccgg aacggcctgg ctggaaagct atgaaattcg
     1741 cctcaatccc gttttgctgt tggaaaacag tgaagctttt attgaagaag tggtaccgca
     1801 cgaactggca catttgctgg tatggaaaca tttcggccgc gtagcgccac atggcaaaga
     1861 gtggaagtgg atgatggaaa gcgtgctggg tgttcccgcc cgtcgtacgc atcagttcga
     1921 actgcaatcc gtgcgtcgca acaccttccc ctaccgctgc aagtgccagg agcatcagct
     1981 taccgtacgc cgccataatc gcgtagttcg tggcgaggcc gtctatcgct gtgttcactg
     2041 cggtgaacag ctggttgcga aataaccatc tgaactatca ggaactttcc tgatctggct
     2101 gattgcatac caaaacaact ttcgctacgt tgctggctcg ttttaacacg gagtaagtga
     2161 tgtaccgtta tttgtctatt gctgcggtgg tactgagcgc agcattttcc ggcccggcgt
     2221 tggccgaagg tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg
     2281 acgcgcccgg tacgttttat tgcggatgta aaattaactg gcagggcaaa aaaggcgttg
     2341 ttgatctgca atcgtgcggc tatcaggtgc gcaaaaatga aaaccgagcc agccgcgtag
     2401 agtgggaaca cgtcgttccc gcctggcagt tcggtcacca gcgccagtgc tggcaggacg
     2461 gtggacgtaa aaactgcgct aaagatccgg tctatcgcaa gatggaaagc gatatgcata
     2521 acctgcagcc gtcagtcggt gaggtgaatg gcgatcgcgg caactttatg tacagccagt
     2581 ggaatggcgg cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag
     2641 ctgccgaacc accagcgcgt gcacgcggtg ccattgcacg cacctacttc tatatgcgcg
     2701 accaatacaa cctgacactc tctcgccagc aaacgcagct gttcaacgca tggaacaaga
     2761 tgtatccggt taccgactgg gagtgcgagc gcgatgaacg catcgcgaag gtgcaaggca
     2821 atcataatcc gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg
     2881 gactcttttt gttaacccct accccacgcg tacaaccgcg tggggagacg acgcggattt
     2941 ttaactatgc gtatcccccg catttatcat cctgaaccac tgaccagcca ttctcacatc
     3001 gcgctttgcg aagatgccgc caaccatatc gggcgcgtac tgcgcatggg gccggggcag
     3061 gcgttgcaat tgtttgacgg tagcaaccag gtctttgacg ccgaaattac cagcgccagc
     3121 aaaaaaagcg tggaagtgaa ggtgctggaa ggcaagatcg acgatcgcga atctccgctg
     3181 catattcacc tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa
     3241 tcgatcgaac tcggtgtaag cctcattacg ccactttttt ctgagcgctg cggcgttaaa
     3301 ctggatagtg aacgtctgaa caagaagctc cagcagtggc agaagattgc aattgctgcc
     3361 tgtgagcagt gtggtcgtaa ccgggtgccg gaaatccgcc cagcgatgga tctggaagcc
     3421 tggtgtgcag agcaggatga aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc
     3481 atcaatacgt tgccgttacc ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt
     3541 ttatcggcag atgaaattgc catgactgcc cgctatcaat ttactgatat cctgttggga
     3601 cctcgcgttt tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct acaagtacga
     3661 tttggcgatt tgggctaacg gagaagaata atgatcaagc tcggcatcgt gatggacccc
     3721 atcgcaaaca tcaacatcaa gaaagattcc agtttcgcta tgttgctgga agcacagcgt
     3781 cgtggttacg aacttcacta tatggagatg ggcgatctgt atctgatcaa tggcgaagcc
     3841 cgcgcccata cccgcacgct gaacgtgaag cagaactacg aagagtggtt ttcattcgtc
     3901 ggtgaacagg atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa agacccgccg
     3961 tttgataccg agtttatcta cgcgacctat attctggaac gtgccgaaga gaaagggacg
     4021 ctgatcgtta acaagccgca gagcctgcgc gactgtaacg agaaactgtt taccgcctgg
     4081 ttctctgact taacgccaga aacgctggtt acgcgcaata aagcgcagct aaaagcgttc
     4141 tgggagaaac acagcgacat cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt
     4201 ttccgcgtga aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct gactgagcat
     4261 ggcactcgct actgcatggc gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc
     4321 gtgctggtgg tggatggcga gccggtaccg tactgcctgg cgcgtattcc gcaggggggc
     4381 gaaacccgtg gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct gacggaaagt
     4441 gactggaaaa tcgcccgtca gatcgggccg acgctgaaag aaaaagggct gatttttgtt
     4501 ggtctggata tcatcggcga ccgtctgact gaaattaacg tcaccagccc aacctgtatt
     4561 cgtgagattg aagcagagtt tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa
     4621 gcacgtttac agcagcagta acccacctta gcgagaagga tctcgttgag actctgagtg
     4681 acagcgccct tctttccacg catactgggc gctgttgctt ttttgaacca ggaaacagaa
     4741 cctctgacaa tgaatttaca gcatcacttt cttattgcca tgcctgctct ccaggatccg
     4801 attttccgtc gttccgtggt ctacatttgc gaacataata ccaatggtgc aatggggatc
     4861 atcgtcaaca agccgctgga aaatctcaaa attgaaggga ttctggaaaa gctgaagatc
     4921 acgccggagc cgcgtgatga atcaatccgt ctggataaac cggttatgct cggcggtccg
     4981 ctggctgaag atcgcgggtt tattttgcat actccgccct ccaattttgc ttccagcatt
     5041 cgcatttcag acaacacagt gatgaccact tcccgcgatg tgctggaaac gctcggcacc
     5101 gataaacaac cgtctgacgt attggtggct ctgggttatg cctcctggga aaaaggtcaa
     5161 ctggaacaag aaattctcga taacgcgtgg ctaacggccc cggcggatct gaatattctg
     5221 ttcaaaacgc cgattgccga ccgctggcgc gaggcggcaa aactgattgg tgtggatatt
     5281 ctcaccatgc ctggtgtggc agggcacgcc tgatgagtgg aaccttactc gccttcgact
     5341 tcggcaccaa aagcattggc gtagcggtcg gccaacgcat taccggcact gctcgccctt
     5401 tgcctgcaat taaagcacag gacggtacgc cggactggaa cctcatcgaa cgtttactga
     5461 aagagtggca gccggacgaa atcatcgtcg gtttgccgct gaatatggac ggcaccgagc
     5521 aaccattgac tgccagagcg cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg
     5581 aagtaaagct ccatgacgaa cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac
     5641 agggcggcta tcgggcgctc aacaaaggca aagtcgactc tgcctctgcg gttattattc
     5701 tcgaaagcta tttcgagcag ggatattaag gcgatctaaa agcctggcgg atagtcgtta
     5761 ccgccatctt gccggatgcg gcgtgaacgc cttatccagt acacatctgg cagcggctgt
     5821 aggtctgata agacgcgtta gcgtcgcatc agacatttat tgcctttata ggccctataa
     5881 acttgcgcat cgggcatggc aacgtcacaa acgcccttcc cccacccgct gctgataact
     5941 ctgctgaaac gttaacatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg
     6001 ggttttccct tcgcgaatca aattccccac cgcgggagtg ttaatcaata attcaaacag
     6061 cgccacgcgt ccttcctgtt tatccacttc cagcttttgt gacaacacgg cccgtaaact
     6121 acctgccagt tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg
     6181 ctcaactgcc tgcgcggcac cacgcgtatg taatgttgcc agcaccaaat gcccggtttc
     6241 tgccgccgtc agtgccagac ggattgtctc gctgtcacgc agctctccga gcaaaatcac
     6301 atcaggatct tcccgcaatg cggcccgcaa tcccgatgcg aacgtcatac agtgcaaacc
     6361 aatttcccgc tgctggatca aacatcgctg gctggcatag agatattcca caggatcttc
     6421 cagcgtcaga atatgcgcat cggcatgttg attaagatag ccaaccatcg ccgccagcgt
     6481 ggtagatttg ccactccccg tcgcccccgt caccagaatc aggccattct cgctcttgag
     6541 taattccggc aataccggtg gcgcaccaag ctgttcgagc tgtggacagt gcgaaggtaa
     6601 caaccgtaat gccagagaaa taccttgccg ttgcgcgaac gcactgccac gcaaccgctg
     6661 gttttccgcc agcgacacgg caaaatccag ctggccattt tccagcaata ttgcccgctg
     6721 atcgtcatcc agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc
     6781 ttccattagc ccgcgaatgc gccatcgtgc gggccaggcg ctgcacaggt gtagatccga
     6841 gacattatgc tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga
     6901 aaatgaacga tattgcgcat aacctggcac aggtccggga caaaatctca gctgctgcaa
     6961 cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg
     7021 cgagcgccat cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc
     7081 aggaaggggt agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc
     7141 attttattgg cccgttgcag tctaataaaa gccgcctggt ggcagagcat ttcgactggt
     7201 gtcataccat cgaccgtttg cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac
     7261 ttccccctct taacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga
     7321 ttcaactggc agaactggac gagctggcag cggcggtcgc tgaactaccg cgtttacgtc
     7381 tgcgcgggct gatggcaatc cctgcgcctg agtcagaata tgtaaggcag tttgaagttg
     7441 cacgccaaat ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc
     7501 tctctctggg aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc
     7561 gtatcggcac tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa
     7621 cgccatgaat acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct
     7681 gttattacgc atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt
     7741 tgtagtgaag gtaacgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg
     7801 tcccattgat agcgcctcgc tgctggttgc gtacattctc agttttatca aagccatcgt
     7861 gctgtttaaa gtggtgacct tcctgccaat catctggatt gccggtttac tgattctgct
     7921 gaaaaccatc ggcctgctga ttttctgggt cctgctggtg atggcgatta tgagctgggt
     7981 aagccagggt cgtagtccga ttgaatacgt gctgattcag ctggccgatc cgctgctgcg
     8041 cccgattcgc cgcctgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt
     8101 tctgctgctg tatgtcatca atatgggtgt cgcagaagta ttacaggcaa ccggaaacat
     8161 gctgctgccg gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg
     8221 gttttacggc tttatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc
     8281 gacgaagtta aagtcgccat taccgcgccg ccggttgacg gtcaggccaa cagccatctg
     8341 gtgaagtttc tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc
     8401 gaacttggcc gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc
     8461 gcggcgttaa ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg
     8521 taaagtgcgt gagctggcgt cgctgcttag cgacttcggt cttgatatcg tggcccaaac
     8581 agacctcggc gtagattccg ctgaagaaac cggcctgacc tttatcgaaa acgcgattct
     8641 gaaagcgcgc catacggcaa aagtgaccgg tttaccggca attgccgacg actctggtct
     8701 ggcggtagat gtacttggcg gcgcgccggg catttactcc gcgcgttatt ccggtgaaga
     8761 cgcgaccgat caaaagaatc tgcaaaaact gctggaaaca ctaaaagacg taccggacaa
     8821 ccaacgtcag gcgcgtttcc actgcgtact ggtatatctg cgtcacgcgg aagatcccac
     8881 tccgctggtg tgccacggca gctggccggg catgattact cgtgaaccag cgggtactgg
     8941 cggctttggt tatgatccaa tcttcttcgt accttccgaa gggaaaaccg ctgccgaact
     9001 gacccgcgaa gaaaagagcg ccatttccca ccgtggtcag gcattgaaac tgctgctgga
     9061 cgctttacgt aatggttaaa ttaccgccgc taagtctcta cattcacatc ccgtggtgcg
     9121 tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg
     9181 acgattatgt tcagcatctg cttaacgacc tggacaacga tgtggcttac gctcagggcc
     9241 gtgaagtaaa gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga
     9301 tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta
     9361 ctatggaagc gaacccaggc acggtagaag cggatcgctt tgtcgattat cagcgtgctg
     9421 gtgtgaaccg catctctatt ggtgtgcaga gttttagcga agaaaagctg aaacgacttg
     9481 ggcgtattca tggcccgcaa gaagcgaaac gagcggcaaa gctggcgagc ggtttagggt
     9541 tacgtagctt taaccttgat ttgatgcatg gactaccgga tcaatcactg gaagaggcgc
     9601 ttggcgatct acgccaggcc attgaactga atccgccgca tctttcctgg tatcaactga
     9661 ccatcgaacc caatacgctg tttggttcgc gaccaccggt gctgccggac gacgatgcgt
     9721 tgtggaatat tttcgaacag gggcatcagt tattaaccgc agcgggttat cagcaatatg
     9781 aaacttccgc ttacgccaaa cccggttatc agtgccagca caatctcaac tactggcgct
     9841 ttggcgacta catcggtatt ggctgcggcg cgcacgggaa agtgaccttc ccggatgggc
     9901 gcattctgcg taccaccaaa acgcgtcatc cgcgtggttt tatgcaagga aggtatctgg
     9961 aaagccagcg tgatgtcgaa gccgcagata agccgtttga gttctttatg aatcgcttcc
    10021 gtctgctgga ggccgcgccg cgcgtggagt ttattgcgta taccgggctt tgcgaagatg
    10081 tgattcgccc acagttagac gaggcgattg cccagggtta tctcacagaa tgtgcggatt
    10141 actggcag
//
LOCUS       DAMZII010000167        10040 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZII010000167 DAMZII010000000
VERSION     DAMZII010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.167"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            72..1850
                     /locus_tag="POA77_004221"
     CDS             72..1850
                     /locus_tag="POA77_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDI6367566.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1890..2369)
                     /gene="xdhC"
                     /locus_tag="POA77_004222"
     CDS             complement(1890..2369)
                     /gene="xdhC"
                     /locus_tag="POA77_004222"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDI6367567.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVQDCEKTK"
     gene            complement(2366..3244)
                     /gene="xdhB"
                     /locus_tag="POA77_004223"
     CDS             complement(2366..3244)
                     /gene="xdhB"
                     /locus_tag="POA77_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDI6367568.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3255..5552)
                     /gene="xdhA"
                     /locus_tag="POA77_004224"
     CDS             complement(3255..5552)
                     /gene="xdhA"
                     /locus_tag="POA77_004224"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDI6367569.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5967..6722
                     /locus_tag="POA77_004225"
     CDS             5967..6722
                     /locus_tag="POA77_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDI6367570.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6801..6874
                     /locus_tag="POA77_004226"
     tRNA            6801..6874
                     /locus_tag="POA77_004226"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6833..6835,aa:Gly,seq:ccc)
     gene            7108..8718
                     /locus_tag="POA77_004227"
     CDS             7108..8718
                     /locus_tag="POA77_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367571.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAQTHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            8731..8850
                     /locus_tag="POA77_004228"
                     /pseudo
     CDS             8731..8850
                     /locus_tag="POA77_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            8850..9794
                     /locus_tag="POA77_004229"
                     /pseudo
     CDS             8850..9794
                     /locus_tag="POA77_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            9787..>10040
                     /locus_tag="POA77_004230"
     CDS             9787..>10040
                     /locus_tag="POA77_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6367572.1"
                     /translation="MSNTISLIAILTLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGTEIYYNSQNENLSFNNV"
BASE COUNT         2769 a         2193 c         2444 g         2634 t
ORIGIN      
        1 atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg
       61 aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa
      121 ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg
      181 aaaacttgtg tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt cgccagttga
      241 gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac
      301 agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag
      361 catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg
      421 ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg
      481 ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata
      541 acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg
      601 atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtgttgtac
      661 aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag agtaatttta
      721 ctcatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta atcattggtc
      781 aattgcataa cattcagggc cgacaattat ttttaatggc atttcaccaa tcgcatacca
      841 gtttttctgt agcaaatgca cctgatgaac cacatattga acaattggtt ggcgagtgcc
      901 gtgttatgcg gcaattaaaa cgactcatta gccgtattgc acccagccca tccagcgtta
      961 tggtggttgg tgaaagcggc acgggtaaag aagtcgtcgc ccgagcaatc cataagttga
     1021 gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg gagcagcttc
     1081 tggaaagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct gccaacggta
     1141 aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata
     1201 tgccattaat gttgcaggct aaattactgc gcgctattga ggcgcgtgaa attctgccga
     1261 ttggtgccag tagcccaata caagtcgaca ttcgcatcat ttctgcaact aatcagaatt
     1321 tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga cttaatgtta
     1381 tcccgataac tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta ttggtgcatt
     1441 actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt gctcccgatg
     1501 tcgtcgaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta agcaatttga
     1561 tggaatatct ggttaacgta gttccttcag gtgaagttat cgacagcacg ctattgccgc
     1621 caaatctgct gaataatggc acaacggagc aaagtgatgt aacagaggtc agtgaggcgc
     1681 acctgtcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag caaatgatcc
     1741 gcgaggcgct ttcacgtcat aacagcaaga agcaagttgc tgatgaactg ggcatcggca
     1801 ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa cctcagtgaa
     1861 tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc ctgaactgta
     1921 tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt
     1981 tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc
     2041 aggccaggcg tacaaaatcc gcactgcact gcgccggatt tcgcataagc ctgctgaaca
     2101 tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc
     2161 caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccaacac cgtacaggca
     2221 ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgttc gcggagtaat
     2281 tccgagagcg gcgtgcctgg tgcggcgtga agctgaaaag gcatcccgtt aatggtgcat
     2341 tcgatggtaa ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg gcgacggctt
     2401 cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc
     2461 gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct tccagcgttt
     2521 gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg
     2581 gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat
     2641 gtgcggcgca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg
     2701 cgctgcccgc gtgttctttc ggctgtggcg gaaaatgaaa ggcgacgagg atttcgtcat
     2761 gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg aaacgaacac
     2821 cgcgcgggga gtggatctcc agtttcgcgt cataaattag cgttggcgtg gcagaatctg
     2881 cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg atctgcggtc
     2941 cagcaatgga cgtggccgca gcacataacg ccgggagatg acgttgagtt ataggatctt
     3001 ctattagctg ggtaaatgtc gttgcagagc cgatacgtag cgagccatct tccgccagcg
     3061 taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt
     3121 gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt
     3181 cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa
     3241 acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa ccgttttggc
     3301 gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagca
     3361 gcggcaacag gaattattgg tggctcaccc agtgacttat gtccgtatgc ggattgcggc
     3421 tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg
     3481 taatccagca gattggggtt acggaccacg ccgcttttag catcgatgat catctcttca
     3541 aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc tgccagcagt
     3601 ggattaagaa tatgccctga atcatgaacg ttgaggatgc ggttgatgat gactttgcac
     3661 agcgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg gttagtggtg
     3721 gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc
     3781 atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg
     3841 ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg
     3901 attttctctt ttaataatag tgccgcactg cgcagcgcag gcgcggcaac atagctctgg
     3961 cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgagt tgaaataacg
     4021 cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa gacggtgtcg
     4081 gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc
     4141 agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt gtagctaaaa
     4201 caggcgacgc caacgccgcg gcgcaaattg ccttgctggt tctggcattc tgcacggcgt
     4261 ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa
     4321 atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg taaacgaatt
     4381 tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat
     4441 acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag
     4501 gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt attccccccc
     4561 gcagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag aacatccaga
     4621 ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat ggtaaaagcg
     4681 tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt caccggaatg
     4741 ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta
     4801 ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc atgaccaggg aatatccagc
     4861 gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt
     4921 cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca atgttgaata
     4981 acgggggtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca
     5041 ttacccgtcg acatcgtgct ttgtttcagt aaattgccac cgttatggat tggtgcagcg
     5101 tcttctgcca gcgccgcttc tggcgtggtg ataacgggta attcttgcca ctcaatgctg
     5161 accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg
     5221 tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt
     5281 tcgtcaagtg tccaggcatg cccagcagta gcaaatggaa tatcaggcac atcttcccag
     5341 gtaaaaatcg ccagtacgcc cggcaaactt ctggcttgtt catcattaat acttacggca
     5401 taaccatgtg cgatagggct acgtacatat ttcgcataac acatgcccgc cataacataa
     5461 tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catcgacgcg catgcatgat
     5521 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
     5581 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt
     5641 taggggaggt aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
     5701 ctgaataggg gcagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaattg
     5761 ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaagttcg atacctctac
     5821 agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt
     5881 accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta tgataaggcg
     5941 tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg
     6001 gtatcgtgat gttgttatcg gttggactgc ttttggcggg ctgttcgggt agcaaatcat
     6061 ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc
     6121 gtatttcgcg caccacggga accagcgtaa aagaactggc gcgactgaac ggcatttccc
     6181 ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta
     6241 gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag
     6301 cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag
     6361 ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctcag
     6421 ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc
     6481 agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt
     6541 acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa
     6601 tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc
     6661 gtgcaacggc aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct
     6721 gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat
     6781 aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc ttcccaagct
     6841 ctatacgagg gttcgattcc cttcgcccgc tccaatttat cttcacctga tcaatctgcc
     6901 atttttgctg tatctgattt tctatattat cttaattcaa tcgcttcaga cttttcgcaa
     6961 tcgccgtaat acttccccat tttgttgtaa ataaaaagcg tttacgcaat gcaatccttt
     7021 tgtaacaacc caggtaaaac accttatttt caagttgtta aataagtggt tatctacgct
     7081 tttaacaaac gattgatgga atattttatg aaagggaaat ctgcattaac tcttctgctc
     7141 gctggtatat tttcgtgcgg cacatgccag gcgacagggg cagaagtgac gagtgaatct
     7201 gtatttaaca ttcttaattc cacgggggca gcaacagata aaagttattt atcgttaaat
     7261 ccagataagt atcctaatta tcgtcttttg attcactcag ccaaattaca aaatgaaata
     7321 aagagccact ataccaaaga cgaaattcaa ggattgctaa cattaacaga aaacacacga
     7381 aagttaacgt tgacggagaa accctgggga acgtttattc ttgcttctac tttcgaagat
     7441 gacaaaacag cggcacaaac ccactatgat gctgtctggc tgagggatag tttatggggc
     7501 tatatggcat tagtatcaga tcagggaaat agtgtagccg caaaaaaggt tctgctcacc
     7561 ctgtgggatt atatgtctac gcccgaccag attaagcgga tgcaggacgt cattagcaac
     7621 cccaaacgac tggatggagt tcccgggcaa atgaatgttg tgcatatccg atttgacagc
     7681 aactcccccg tgatggcaga tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa
     7741 caaaatgatg cgttaggtct ttatcttgat ctccttatac aagcaatcga cactgataca
     7801 attaacgctg aagattggca aaaaggtgac cgattaaaat cggttgcgct gctgattgct
     7861 tatctggata aagccaattt ctatgttatg gaagattccg gagcatggga agaagatgcc
     7921 cgattaaaca catcttccgt tgcactggtg acctctggac tggaacgttt gtcgaactta
     7981 ctctcgaaga aagactctgt ttttgtctct gatttactac gcgaagctaa agccaatgaa
     8041 ctggatgaac ctctttcaac aacgaggttg aaccacctca tagataaagg ttatgaaaga
     8101 ataacgttac agcttgattt agggggagaa tccccaggtt atctggaaaa agataaacat
     8161 tacagagaag ccgatgcggc tttacttaat gttatttatc ctgcaaatct tgcaaagata
     8221 aacaccagaa gaaaagagca ggttctaaaa atagttaaaa aactagcagg tccgtatggt
     8281 ataaaaagat acgagaagga taattatcag tcggcaaatt tttggttcaa tgatattaaa
     8341 accgataccg atcaaaattc acatgcgaaa agagaaaaga gcttcattcc atcaacagaa
     8401 gcagaatggt tttttgactc atggtatgca aaatcagcag caattgttta taaagaatca
     8461 agaaaggaag agtatttgaa tgactcagtg caatttatga atagatcatt ggcccaaata
     8521 acaggcgaga atatgattgg tgcaaatggc aggagtgtac ctgaaatggc acttcccgaa
     8581 agctataact atatccacaa gtcgggaact ttgcatgaag cgccaagccc tatcattccg
     8641 ctaaattggt cgaaagcttc aatgactctc atgttgaaag agatgtccaa ccttatcaat
     8701 gatgagggaa ttaaatagtt ttcataatga gttacagcac aacgttgggt attgactgag
     8761 caagatgaac gacaaggaca acgacatcag ccttatgagg agcaagaaaa tgaaagcaaa
     8821 ttcgaaaacg atcagaagga tgaaagttaa tgttcggtct gaggaaagta aatcgaaaca
     8881 ctcatacgtt tgaaaccact tttcaaaatt ggaaagaaaa tggcgaagat gttgcattat
     8941 taatgcccga atttagtgct aaatggttac cgatagcaga agagagtggc agctggtctg
     9001 gatgggtatt gcttggagaa atatttccct taatttcatc tgagctcgct gggatggctt
     9061 taatgccaga aacggaaagg ttaatagggg aatggctcag tttatccagt tccccgttaa
     9121 atctaaagca tcctgaacta aaatataatc gtttgcacgt aggtaaagta tttgatggag
     9181 tattgagccc tgctcaacca ctaataagaa tatgaacagg ggaattgaat ctttggttag
     9241 ataaagtcac agtctgccaa tacggaaacg ctccagtgtt agacaaaaaa tcattatatt
     9301 ggtccattca ttttgtaatt ggatttagta aaacatgtta cagaagtctt gttgatattg
     9361 aagttggtga tgttttatta atctcaaata atttggctta tgcggttatt tataatacaa
     9421 aaatttgtga tttaatttat ccagaggagt taaaaatggc tgatcatttt gagtacgagg
     9481 aagattttga aacagatgat tttgatatca aaaaaaacga gagtgagatt tatgatgaaa
     9541 atgacgatca gatgattaat agttttgaag acttgcctgt aaaaattgag tttgttcttg
     9601 gtaagaaaat aatgaatcta tatgaaatag acgaactctg tgtaaaaaga ataatatctc
     9661 tgcttcctga atctgagaaa aatatagaaa tacgtgtaaa tggcgcgcta actggctatg
     9721 gcgaacttgt tgaagtggat gataaattgg gcgtagagat tcattcttgg ttatcagggc
     9781 ataataatgt caaataccat ttcgctgata gctatactaa cattatttac actattgccg
     9841 tttataatag catccgggac atattttatt aaattctcga ttgtttttgt tattgtccga
     9901 aacgcgctgg ggcttcagca agtgccatca aatatgacac ttaatggggt tgctttgcta
     9961 ttgtcaatgt ttgtaatgat gccggttggg acggaaatat attataacag tcagaatgag
    10021 aatctttcat tcaataatgt
//
LOCUS       DAMZII010000168         9903 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZII010000168 DAMZII010000000
VERSION     DAMZII010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.168"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..2172
                     /gene="iroN"
                     /locus_tag="POA77_004231"
     CDS             1..2172
                     /gene="iroN"
                     /locus_tag="POA77_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HDI6367573.1"
                     /translation="MNKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQVLK
                     QQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGPEN
                     TLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGAAG
                     GVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGNLN
                     KTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIYAG
                     DTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRMNE
                     GLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDPSS
                     TSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSDSG
                     GNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGGCY
                     LIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAYIL
                     KWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTINNS
                     LNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDINK
                     NLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2217..3173)
                     /gene="iroE"
                     /locus_tag="POA77_004232"
     CDS             complement(2217..3173)
                     /gene="iroE"
                     /locus_tag="POA77_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HDI6367574.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3258..4487)
                     /gene="iroD"
                     /locus_tag="POA77_004233"
     CDS             complement(3258..4487)
                     /gene="iroD"
                     /locus_tag="POA77_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HDI6367575.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4591..8250)
                     /gene="iroC"
                     /locus_tag="POA77_004234"
     CDS             complement(4591..8250)
                     /gene="iroC"
                     /locus_tag="POA77_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HDI6367576.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            complement(8390..9505)
                     /gene="iroB"
                     /locus_tag="POA77_004235"
     CDS             complement(8390..9505)
                     /gene="iroB"
                     /locus_tag="POA77_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HDI6367577.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
BASE COUNT         2320 a         2713 c         2682 g         2188 t
ORIGIN      
        1 attaacaaaa tcctctggtc gctaactgtg ctcctggttg ggttgaatag ccaggtatca
       61 gtagccaaat cctccgacga tgataatgac gagactctgg tggtggaagc caccgctgag
      121 caggtattaa aacagcagcc gggcgtgtcg gttattacca gcgaggatat taaaaagacc
      181 cctccggtaa acgacctttc agatattatt cgtaaaatgc ctggcgtcaa tcttaccggc
      241 aatagcgcct cgggcacacg cggtaacaac cgccagatcg atattcgtgg tatggggccg
      301 gaaaacacct taattttaat tgatggtgta ccggtgacgt cacgtaactc cgtgcgttat
      361 agctggcgtg gagagcgtga tacccgcggt gacaccaact gggtgccacc ggaacaggtt
      421 gagcgtattg aagtgatccg cggccctgcg gcggcgcgct acggttcggg ggcagccggg
      481 ggggtggtga acatcattac caaacgtccc accaacgact ggcacggttc gctgtcgtta
      541 tacaccaatc agccggaaag tagcgatgag ggcgctacgc gtcgcgccaa tttcagcctt
      601 agtgggcctc tggctggtaa tgctcttacc acgcgtttgt atggtaacct gaataaaacg
      661 gatgctgaca gttgggatat taattctccg gtcggtacga aaaacgcagc cgggcatgaa
      721 ggggtacgta acaaagatat taacggcgtt gtctcgtgga aattaaatcc gcagcagatt
      781 ctcgatttcg aagccggata tagccgccag gggaatatct atgcgggcga tacgcaaaac
      841 agttcttcca gtgcagttac cgaaagcctg gcaaaatccg gcaaagagac gaaccgcctg
      901 taccgacaga attatggcat tacgcataat ggtatctggg actggggaca aagtcgcttt
      961 ggtgtttatt acgagaaaac caataatacc cgcatgaatg aaggattatc cggcggtggt
     1021 gaagggcgta ttttagcggg tgaaaagttt acgaccaatc gcctgagttc ctggcgaacc
     1081 agcggcgagc ttaatattcc tttgaatgtg atggttgatc aaacgctgac cgttggtgca
     1141 gagtggaacc gcgataagct cgatgatcct tcctctacca gcctgacggt gaatgacagt
     1201 gatatcagcg gtatttctgg ctctgctgcg gatcgcagca gtaaaaatca ttctcaaatc
     1261 agtgcgctgt atattgaaga taacattgag ccggttcctg gcacgaatat cattcccggc
     1321 ctgcgctttg attatctcag cgactccggc gggaacttca gccccagtct gaatctttcg
     1381 caggaattgg gcgattattt caaagtcaaa gcaggggttg cccgaacctt taaagctcca
     1441 aacctgtatc aatccagtga aggctatctg ctctactcga aaggcaatgg ctgtccaaaa
     1501 gatattacat caggcgggtg ctacctgatc ggtaataaag atctcgatcc ggaaatcagc
     1561 gtcaataaag aaattgggct ggagttcacc tgggaagatt accacgcaag tgtgacctac
     1621 ttccgcaatg attaccagaa taagatcgtg gccggggata acgttatcgg gcaaaccgct
     1681 tcaggcgcat atatcctcaa gtggcagaat ggcgggaaag ctctggtgga cggtatcgaa
     1741 gccagtatgt ctttcccact ggtgaaagat cgtctgaact ggaataccaa tgccacatgg
     1801 atgatcactt cggagcaaaa agacaccggt aatcctctgt cggtcatccc gaaatatact
     1861 atcaataact cgcttaactg gaccatcacc caggcgtttt ctgccagcgt caactggacg
     1921 ttatatggca gacaaaaacc gcgtactcat gcggaaaccc gcagtgaaga tactggcggt
     1981 ctgtcaggta aagagctggg cgcttattca ctggtgggga cgaacttcaa ttacgatatt
     2041 aataaaaatc tgcgtcttaa tgtcggcgtc agtaatatcc tcaataaaca gatcttccga
     2101 tcttctgaag gggcgaatac ctataacgag ccaggccggg cttactatgc cggagttacc
     2161 gcatcattct gatcgtagtg tttcaggcgt actgcccgcg taaaagcggg cagtgtttag
     2221 tggcttaact catgacaacc tgctgtgtaa tttgcgtttt caccactgat atctaacagt
     2281 gcctggcgaa aggaggcatt gaacatcggc ccgtgtccta ggttggggaa atcccaaaat
     2341 acggcattga cgcctttatc tttcagtata gtgagggtgg tatgaatttt cgacagcacc
     2401 ccgacagcat gcgtttcccg gttatcaccc tgtgtcgccg agccttccat tatcgccagg
     2461 tgtttggcgc agaattgcag aggctcaacc gccgtaacgc ggcttagcaa agcatcataa
     2521 cctctgccca acgacgggct ggcgctgtag tacgaccgga agtaagagga ggacagccag
     2581 gaatccagca cgaagaggcc gccgtaggag tgcccccata agccgcggcg ttgccgatcg
     2641 atattcagtc cctgttccac ttttggggca atacgcgttt ccagtaactg gcggaagttg
     2701 ttgctgccac cactcttacg gctaaaacgc cctgagtgga gatctgtttt tctgctttct
     2761 gctgctggcg tatagtcgta agccctgctg ttgagatcga aagggaggtt ggtctgatac
     2821 ccgacagcca cgatcactgg cggtgttttt tctgacaatt gtttgagcag ttcgtcatcc
     2881 aggcgatcca taactgcgtt accgtcaagc atatataaaa tcgggtaacc cgatgccggt
     2941 gcggttgtat tcggcacggc cgtccatacc cgataatggc gtgtgccatc gacagagtcg
     3001 aaagaggtga cgctgaaatg gtaaaacacg gagcctttat cggctatatt cggccccagt
     3061 ggccgcatat ccggcttcgc ataaacttgc gcactacaga taagggtgag gcaagaaaga
     3121 tgaaagaaaa tcgctttatg cgggcgtgtt gagcgatact cgcgggcata catctgatga
     3181 ttattccttt tccgcaggtg actatttgat ttattcagga agtctgtacc gtttcctgaa
     3241 agtgtttgtg ggtcaactca accctgtagt aaaccaatcc cgtcaatcag tgcaccgcgc
     3301 caccatgcgt aatcgtgacc accggtgtag attgcgcaat ggctttcgac gccagcggta
     3361 atcagccgct ggtgaagctg ctgaacgtga ggcactgtcg aaccttccag cgatcccacg
     3421 cacaggctga tacgtacgcc ctgcggtggg gcagaaagca gatgctcact cacccatgag
     3481 gtatcggttt cgctgaacaa gcctggtcga ctggttcttt ctggcgtcca ccacattgaa
     3541 ggagagtggc tgagcaccag accgaacgtt tccggtgcgt aacgagcccc cattagcgca
     3601 ctgatcccgc cgaggctctg cccggccagc actgtgcgcg aacggtctgc ccactgacgc
     3661 tgcggttgtt cagcgcgaat catcggcagc agatgtccgg cgatatcctt tatcagtttg
     3721 ctgcgcccgc cgagtatctc agtgcgttca tgttcattaa tgttgtcaat gcccagtaca
     3781 gccacgggca cgatgcgccc attatttatg gcggcgtcaa ttgccgcgca tacgccaagg
     3841 tgatcaaacc aggtttcacc atcgggcagc acgaccagcc cgaggggctg agaaatatca
     3901 acatccggaa tatagagccg aacctggcga gatttcccgg cgataatgcg catggaggta
     3961 agcagttgtc cggtgtggaa gccgccgttc cattccgaca gggcgggagc tttatcaagt
     4021 gtcagcactg attcctttgc gtttccccga acgttgatct ccggcatttt gtttagcgga
     4081 tcggcctttc cggcaagggt ggcaaaacgg cctccggaca gtgcaatcgt ctcagccgta
     4141 gtgccggggg ggatttccag cagcgaatag gagccgcagt aacttgcggg taaacgcagt
     4201 gtcagtgtcc agatatccgt ttcgggaagg gcgctcatca ttcctttttc tacgtgctct
     4261 ttatccgtca cccggttcag acgcagataa acgccctgca gaggatgtcg ggcgcgccac
     4321 agaaaggtca cttctcgctc atcttccgct ccttcgactg gcgtcacaag aggaacattc
     4381 agcgactcag cttcgcgcca gaagtcagta aggttagcaa tgtggcccgc tgccagttgg
     4441 ttgcgcaggc tggcgatagc agagggatgt tgttgcatgt tcagcattca tactctttaa
     4501 aaattaagga tgtcagtata aataattaaa gcggctattc acggtaccgc cgcttctgac
     4561 tgtgttttta atacattacg tgtccagtaa ctatcccggg gtttcctcct tcatgttgtg
     4621 ctggcgatgg agcgcagtac tgctgacgct gtcatgccat aagcgggtat agaggccgcc
     4681 cgcagataac agctgttcgt gggttccgta ttccgcaagt aaccccttat caataacggc
     4741 aatcagatcg cagcgttgcg cggtggtcag acgatgtgca acaatcagcg ccgagtgctt
     4801 cccggcatgc acgacatctg ttaacgatga catcagtcgt tcttcggatg tgcgatccag
     4861 acatgacgtg gcttcgtcga gcagcaggat gtgggcattc gccagttgcg cgcgggccag
     4921 cgcaatcaac tggcgctgac ctgcgggcag atcggctccg ccgttattca ccggtgtccg
     4981 gaatccctgc ggcagattgc acaccatctc atacagccct gcccgctgtg aggcaatttc
     5041 aacgtcttca ttggtggcgg atggccgtga ataacgaatg ttttctgcaa tatcactact
     5101 aaacagtgct acatcctgat cgacaagccc aatctggcga cgatactcag tgagcgaggc
     5161 atcatcgagc atttgcacac cgattctgat gttgccgtgc gtggggaaat acaacccggc
     5221 aatcagctta atcagcgtcg acttacccgc accgctgcga ccgacgaccg cgacaaccat
     5281 tccctctggg atcgtcaggg taagtttgtt cagagctggc tcgtgactgt cgggataact
     5341 gaacgtgacc tcatcaagat gcagtgcacc ggtgacaggg aggaccgaag aggacccgag
     5401 gttctcagtg ccttctgtcg ccagtagttc atcaatatgt ttgccgctgg ctgtcgcctg
     5461 ctgccaggcg tcgacaatcc ctgataactg ctgcactggc ccatagaatt gccccagcaa
     5521 caggaagaaa gccgccagta ccccggcagt catttctcca gcggcgacct gcgaagctcc
     5581 cactaacaga acagcggcat aggaggcctc ggtgcagaat gtcaggaacg gaaaatagac
     5641 tgcaaggtat ttttgtgctc gcacacgggt tgcgcggaaa cgctccgata acgcgcgcag
     5701 ccgggcgccc tccagttcct gctgaccatg cgattgcacg acacgcaggc cagagacttt
     5761 ttcctgcagg gtgctgttga ctttgcctat ttccagccgt gcctgtgcat aagcaggggt
     5821 actgaggcgg cgataaatca tggttgccag tgcggccact ggcactgcgc ttaatgccgt
     5881 cagcgccagg aacgggtcga gccagaacat ggtggcggcg attgcgaaca tcgtcaccag
     5941 actggtggcc gcaccggcaa ggccgttttg cagaaagcgg gcgagagagt ccacatcaac
     6001 ggtcatgcgg gtaagacgcg aatcggcatg cttttcatgc caggggagtc caagacgcag
     6061 catatggccg aagctgcgca agcgcaccga atgctgaact gattccgccg ctctggcggc
     6121 gaagaccgtc tgcagagaat aactgcacca gcccacaatg accaggcaca gggcgagcag
     6181 ggcacagagg ccgatgatcg acatatcgcc tgcggcgaca ccggagtcaa tgccgtgctg
     6241 caacagtatc agtaccccta cgcctgccgc agaatcgagt gcgatcagca gagcggcgat
     6301 gacgaacatc cacgccacag gcttcagtag tgacgtgact ttgcgacctt taccggctag
     6361 cgcctgctgt actgcatttt ccggtacgcg ggtcatacgg gcgacaaaac ctttatcccc
     6421 cgtatccgtt acatcgtctt gtgtcgctgg ttcagccggc cacaattcgt tgtggctatt
     6481 gtgggacgtg gccagaaaat cgctgtcgcc ggtcatcagt gcgcggaacg ccggacagcg
     6541 cgcttcaagt tctgccgggg ttccggtatc caccatacgg cccttatcca gcaccacaac
     6601 ccggctggct agctgaagtg ttgaacgccg tcgggcaatc accagcagca tatgcccttc
     6661 gtcagcatag cgacccagcg cggtattaat ctccgcttcc gtaacagcat caaccgctga
     6721 ggtggtatca tccaggatta acacgtccgg tgcagtaatg agcgcccgcg ccagcgctat
     6781 tcgctgcctc tgcccgccgg aaagattcgt accccgttcg gttaacaggc tatcgaagcc
     6841 tttcggcagg gcgttgataa aatcgctggc tcctgcagca gctgccgcac ggcgaatgtc
     6901 gtccgccgtt gcctgagggt ggccataggc gatattttcc gccaccgtac cggcaaacag
     6961 aaaagcatct tcaaatacga tgcctacccg gcgacgaagc gcctccagtc tgatatcgcg
     7021 aagattttgt cggccttcgc tggtgttgag ccatatcttt ccggagcagg gatcataaaa
     7081 acgcgccagc agcatcaaca gggtcgattt tcctgaaccc gatgcgccca ccaccgccac
     7141 ggtttcaccg gcgcgcaggg aaaaggagat attgctgagg atatgtctgt cgccatggtg
     7201 gtagtcaaag ctcatattct ccagttccag tccgacaacc tgactgttaa tcgactccgt
     7261 cccgtcctcc atctgtgaac gggtgtcgat cagtgcaaac acccgctcca cggacgcctg
     7321 agtgcgctgt gcgataacca gaaacgatgc cagtacgcgg gttggcccgg tcagcatcgc
     7381 gaggaagctg gcgaatgcaa cgaaggtacc gagatcgatc cgcccgtgca tgaccgacca
     7441 cccgccgtag cacagcagga cgatttgccc gagcaccggt agcgccagca tggtggcccc
     7501 aggcatcgcc tgcgagataa ccgcaccgat gcgtacacgc acaatctgcc gcgactgcct
     7561 atcgagccag tgcgtttcac gcatctctgc cacacaggat tttaccactg agatctgtgc
     7621 cagcacctcg cgtatatgtt cggtgagatt cgccagttgg tccgaggcca gcccggtttg
     7681 cgcgaagacc ctacgacgcg cacgaagcgc ggtgatcgcc aggcatacca gtacgcacac
     7741 gacgataagc gtcatggcag gggacatcca cagcatcacg gcaatgccgg caatgtaata
     7801 agtgaacact gccagcggca ccgggcacat ctgcagcagg gtatgcacct gctgcagatc
     7861 gctgttggtc cgtgaaatca cctgcccggt gcgcagcgca tcctggcctg cgccgtcaag
     7921 tttctgaata ctgtcgaata cccgtcctct gagggtatgc tgcacccaga gtgagagcat
     7981 cccggcataa ccacggcgca catagttacc gatgaaatca aaaaaagcga tcaacagtaa
     8041 cccgcaggcc agccacggca ggcgggccgg attgcccgcc agtgcgtcat tcacagcctg
     8101 tcttgtcagc agtggcgtca gcgcagccag caaaatagtc gatactgacg ctaccaccac
     8161 gatgacacta agtttcttac gttcccagca cacgcgcgct aggcgaacta tccatgactg
     8221 agccggtgtg ggagtgtgat tcgcaggcat taagcctcct ccgtctgtaa aatacgatcc
     8281 actggccgga tcgttccgca aaaaagccag cacgctccgg tcccgcattg ttatggatgc
     8341 atgactcctg gatgggtgct ggcatatgat tatcatgaag gtgtagtggt taccctttct
     8401 gtaccattgt gatcaggctt ttcgccactt cacctgggca gggttgcgcc gccatttccg
     8461 ctgctacctc ttcagaggct ttgcgaagtg agcgattgtt gaggaaagca ttgatcatgt
     8521 tactcgacag gccgacgtcg ccagggataa tcccgcagcc gcgttcggca acaacgcggg
     8581 catttaccgg gcgatcggct ccctggccga agacgatttg tggaatacca gcgtgtagcg
     8641 ctgtcagggt attgcctgct ccgccatgat gaatgaaacc atctgcgccg ttgaggaaga
     8701 cacccatggg tatccagtca accaggcgaa catttgacgg cagcgagcgc aaatcggacc
     8761 gcgcattggc tgaaatatgc aggatgatct cggcatcgac ctcactggca gaatccataa
     8821 cccaggcgat tagatcaagg ccgtcaacca tcggtttgac agtgccgaga ctcaccagca
     8881 gacgtttacg ctcgggcctt ctctcccacc atggttccca caccgcgcca ccgttatacg
     8941 ggacgtactg catcggaata atgggttcac cgtcgttttc cagaatgctc atactcggcg
     9001 gtgttacgtc gatccaggcc atatcacgtg gtgtggcccc cacattgtgg cgacgatagg
     9061 cgtccgtgag cgaacgcgtt acacccttga tatgccaggg ggtatggccg aagcctacgg
     9121 tttgcatcac gaccggtata tcatatttgg cggcaatcag cggcccgatg actccgagcg
     9181 gagggtagat gatgaggtca ggccgccagt gtccggcaaa ctccaccaga tggtcggcca
     9241 tttcttcgct gaagaatgag aagttgccca tttgggttcc gatattactt tttttccgct
     9301 gtgcctcatg gtggcggtaa ccagcttcgg aatccagacc gggtgcggcg tcaaacacca
     9361 ccaacccggc ctctgccgct ttttgtgcga attgtcctcc actggcgatc agcacttcat
     9421 gaccgttaac gcgaaatgcc tgcgccagag acagcacagg atacaacagc ccatagagag
     9481 gcgggcctac aaacaaaata cgcatgagaa aatccctctc cgcttgacga tctaaatcag
     9541 gcagtcgacg cactcatatg ccagcagcag gacacgactt ttcgttaaac ctgctttacc
     9601 agatgctgcg ccatttgtgc ttcaccagcg ttatggacag ctcagtcaga gacttttccg
     9661 tgacatacac aggtgaacag ggaactccgt atccccactt ccattaacgc ttacgcgcca
     9721 ttgtcctgtt gaacagccca aatggcgtcg gacagctacg ccgggatgtt ctgacgcatt
     9781 tgtgcagtgt tagaccgaac aacgaatctg gaaagaaccg tgatttctta gacacacgat
     9841 gcatcatcct gatagtctcg ccttcgctgg tctgtaagta aaaacgccgt tgtacaggcg
     9901 atg
//
LOCUS       DAMZII010000169         9829 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZII010000169 DAMZII010000000
VERSION     DAMZII010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.169"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            4..564
                     /locus_tag="POA77_004236"
     CDS             4..564
                     /locus_tag="POA77_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367578.1"
                     /translation="MIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIA
                     RYVATNATVTAGTANADSQFTVEYKK"
     gene            612..1343
                     /locus_tag="POA77_004237"
     CDS             612..1343
                     /locus_tag="POA77_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6367579.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFGKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1368..3890
                     /locus_tag="POA77_004238"
     CDS             1368..3890
                     /locus_tag="POA77_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6367580.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3901..4974
                     /locus_tag="POA77_004239"
     CDS             3901..4974
                     /locus_tag="POA77_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367581.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5222..6262
                     /gene="pstS"
                     /locus_tag="POA77_004240"
     CDS             5222..6262
                     /gene="pstS"
                     /locus_tag="POA77_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI6367582.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6349..7308
                     /gene="pstC"
                     /locus_tag="POA77_004241"
     CDS             6349..7308
                     /gene="pstC"
                     /locus_tag="POA77_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDI6367583.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7308..8198
                     /gene="pstA"
                     /locus_tag="POA77_004242"
     CDS             7308..8198
                     /gene="pstA"
                     /locus_tag="POA77_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI6367584.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8289..9062
                     /gene="pstB"
                     /locus_tag="POA77_004243"
     CDS             8289..9062
                     /gene="pstB"
                     /locus_tag="POA77_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDI6367585.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9077..9802
                     /gene="phoU"
                     /locus_tag="POA77_004244"
     CDS             9077..9802
                     /gene="phoU"
                     /locus_tag="POA77_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI6367586.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2521 a         2265 c         2510 g         2533 t
ORIGIN      
        1 aatattatag gcggtgcatt cactctggca tctctaatgc tggccgggca tgcactggca
       61 gaagatggtg ttgttcactt cgtcggtgaa attgtcgaca ctacttgtga agttacctcc
      121 gatacagccg atcaaattgt cccactgggt aaagtcagta aaaatgcatt ttcaggtgta
      181 ggtagtctgg cgtcgccaca gaagttcagt attaagctcg aaaattgccc ggcaacgtac
      241 actcaagcag ccgttcgttt tgatggtaca gaagcgcctg gcggcgacgg cgacctgaaa
      301 gtgggtacgc cgcttacagc aggcaaccct ggtgatttta ccggtacagg acaagcgatt
      361 gcggcaaccg gcgttggtat tcgtattttt aaccagtccg ataattcgca ggttaaactt
      421 tataacgact ctgcttatac cgctatcgat gctgaaggca aggctgaaat gaagtttatt
      481 gcacgctatg tggcaaccaa tgcgaccgta acggctggta cggcgaacgc tgattcacaa
      541 tttactgtcg aatataagaa ataatttatc aagggccttt ttattcagtt ggctttggcc
      601 agggagtcgt tatgtccaga aaaagttgtt taatcaggct ctttaaaaca acagtttcat
      661 gtaagttatt taccgcgatt atactcagcg tttttttagg ccaaccagca ttaacttatg
      721 caggcgttgt cattggtggt acgcgggtgg tttacttaag taataatgct gataaatcta
      781 tttctgtatt tggcaaagaa gaaaaaatac cttatctcat ccaggcttgg gtagatccat
      841 ttaataaaga ggataaaagc aaagcgccgt ttaccgttat tccgccagta tcacgattag
      901 aaccttcgca agaaaaaata ttacgcatta ttcacaccaa aggtgtgtca ttgcctgatg
      961 atcgcgagtc agtcttctgg ctgaatatta aaaatattcc gccatcagcg agcaacaaag
     1021 cgacgaactc gctggaaata gcagtaaaaa cgcgtatcaa gctgttctgg cgaccagcaa
     1081 atatccgcct tattcccgaa gatgctgcgc cgaaagtgaa atggcgccgg gaagggcgaa
     1141 atctgatagc agagaatccc aacccaattc atatcagtgt gatggatgtc atcgttgatg
     1201 gccatgatgt gcccctcaat atgatccgtc cttttgaaac gctcacgtta cctttacctg
     1261 ctaattctgc gggaggccaa atgacctggc gatttattaa tgattacggc gcagtcagtg
     1321 acccgataaa aatgacgctg taagcaaggg ataaggatag gcgtattatg atgacgacca
     1381 gaatagtggt tggcctcacg gcagggacgt gtctgatttt ctcgcaaaac ctgatggccg
     1441 aggtcagtgt attcaatccg gcgcttctgg aaatcgacca tcaatccgga gtcgatattc
     1501 gccagtttaa tcgggcaaac ctgatgcccc caggtgttta tagcgttgat atttttatca
     1561 acggtaaaat gtttgaacgt caggatgtga catttgttca ggataatcca gatgctgatc
     1621 tgcacgcttg ctttattgcc attaaaaaaa cactgtcctc ctttggcata aaagttgatg
     1681 cgctcaaatc gttcaatgat gtggatgaga cggtttgcct cgatcctgct ccacgtattg
     1741 aaggctcatc ctggcagttt gacagtgata aattgcagct gaatatatcc attccccaaa
     1801 tctacatgga cgcgatggct tatgattaca tcagccccac gcgttgggat gaggggatta
     1861 acgcgctcac catcaactac gatttttctg gttcacatac actacgttca gattatggtt
     1921 cacaagagac agataccagt tatctcaatc tgcgcaatgg actgaatatt ggaccgtggc
     1981 ggctacgtaa ttacagtact ttaaacacca gcgatggccg tgcggaatac aactccatta
     2041 gtacctggat acagcgcgat attgccgcgt taagaagcca gattatgatt ggtgatacgt
     2101 ggacggcgag cgatattttc gacagtacgc aaattcgcgg cgcgcgtttg tatactgata
     2161 acgatatgct acccgccagc cagaatggct ttgctcctgt ggttcgtggg attgcaaagt
     2221 ccaacgccac cgtcatcatt cggcagaatg gctacgtgat ttatcagtct gccgttccac
     2281 aaggtgcttt tgagatcacc gatctcaaca ccgccagtac aggtggcgat ttggacgtaa
     2341 ccatcaaaga agaagacggt agcgaacaac gattcaccca accttatgct tcattggcga
     2401 ttcttaaacg tgaaggtcag acagatgttg atgtcagcgt gggtgaattg cgcgatgaag
     2461 acggatttac accggacgtc cttcaggcgc aaatacttca tggtttttcc cacgggatca
     2521 ctttatatgg aggtatgcag gctgctgaaa attatggttc tgcagctctg ggtgtcggta
     2581 aagatcttgg cgctttgggc gcaatttctt tcgatgtgac acatgctcgt gcgaatttta
     2641 gccatgatga tacagaaacg ggtcagtcat atcgctttct ctattcaaaa cgatttgacg
     2701 acacagacac tagcttgcgc ctggttggct atcgttactc caccgagggc tactataccc
     2761 tcaatgagtg ggcatcgcgg cgcaacagcc ctgaagactt ttgggaaaca ggtaaccgac
     2821 gtagtcgcgt ggagggaacg ctaacgcagt cgttggggag agattatggc aatttatacc
     2881 tgacattaag ccggcaacaa tactggcata ccgatgatgt cgaacgatta atgcaatttg
     2941 gctacagcag tagctggaag cgtctctcgt ggaacgtctc ctggagttat tccaatactg
     3001 ccagacaggg gacggggaac aaccatgcca gtgataacac cagtgagcag atctacatgc
     3061 tctctttatc tgttccttta tcgggctggt ggggtaatag ttacgccacc tattctgttt
     3121 cgcaaaacga taattccggt agctcacatc aactcggact cagcggtacg gcgctggaaa
     3181 gaaataacct ttcatggaat ttaatgcagt cctataacag tcatgatgat gaggttggcg
     3241 gtaatatgtc cctgacctat gatggctctt atggcacggt gaacggcagc tataactaca
     3301 gccaaaattc ccagaggctg aattatggta tcagaggggg aattctggca cacagcgaag
     3361 gggtaacgtt aagtcaggag ttaggtgaaa ctattgctct tgttaaagca cctggggccg
     3421 ccgggttaga aatagataat atgcgcggtg ctgcgacgga ctggcgcggc tatacggtca
     3481 agacacagct aaacccttat gatgaaaatc gggtagcaat cagcgataac tatttctcga
     3541 agtcgaatat agaacttgat aataccgtcg ttacgatggt tcccacgcgt ggtgcagtgg
     3601 ttaaagcgga gtttgtgact catgtgggtt atcgcgttct cttcagagtg ttaaatgcaa
     3661 atggtaaacc ggtacctttt ggagccattg ctgcgataca agatgcaagt ttggcagatt
     3721 caggaattgt cggtgaccgt ggcgaacttt atctttctgg tctaccagaa aaaggacagg
     3781 ttacgttatc ctggggagaa aacgcctcaa caaaatgcat cttcaattat tcactttcga
     3841 caccagaaag tgagagcgga ttaattgaac agggtgtgac atgtcattaa aaggattttt
     3901 atgaacaagt atatcaaaca gtggtgcttt gctgtgttta tgctctcgtt aagtagtgta
     3961 gcacttgcag ctcctaaagg tatctgtacc cctgataatg gcgtatttca cagtacgctt
     4021 gatttctcgg gatatcttat taccgcgaac gaaaataagg ttggaacgac atttaacacc
     4081 actgtaacta atggatcctc atatcctgga cgctgtcatt gtgatactgg taacgtagga
     4141 gaattcccct atatctatta tacgtcaaaa ataaaccagg cattaactta tgctggggtt
     4201 cactctaata ttaattatta tgatcttaat ccgaaccttg atgttgggat tgctatagat
     4261 attcttgggg ttgggtatgt gaatgcacct tttgaatatc atgctaacaa cccgtctggt
     4321 aatacaaagt acaattgcaa ccgcattgaa cctttaagta tatctagtgg ggctaaagcg
     4381 atagtatatt tttatatcaa gaagacattt gcaggaaaat tgattattcc tgaaacaaag
     4441 atagtgacat tgtatggaac aattagccgt gacaccccgg tggattactc acagccgatg
     4501 gctgatgttt atattcgggg cgatattaca gctccgcaaa gttgcgaaat aaataattta
     4561 cagccagttt attttgattt taaagaaata ccagctgcag atttttcatc tgttgttggg
     4621 agtgcggtaa caacacataa aattactaaa acagtcacta ttgagtgtga aaacctgggg
     4681 atactgaata ccgatgatat cagtacgtct ttttatgcta ccgaacccaa tacggacaat
     4741 tcaatggtcg taacttcaaa ctctaacgtg gggataaaaa tttacgacaa aaataataag
     4801 gaaatcaaag tgaacggtgg tgagttgcca acagacatgg gtaaatcaac agtgtatggt
     4861 gaaaaatcag gtagtgtaac tttttcagct gctcctgcga gccttactgg agctcgccca
     4921 gccccggggc aatttactgc aacagcgacg ataactgttg agattgtgcg ctaataggtc
     4981 tgacaacgat tgttcatcac atcgttgtca tctcacccat ttctttatca cttttaaatg
     5041 catcatgaca aaaagatgac atgtgggatt ttcatcttca catttaaaat cagtaagtta
     5101 tttggtaatt cctttctgtc ataaaaatgt catattcctt acatataact gtcacctgtt
     5161 tgtcctattt tgcttctcgt agccaacaaa caatgcttta tgaatcctcc caggagacat
     5221 tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat cgatgagcgc
     5281 tttctctgtg tttgcagaag caagcctgac aggtgcaggt gcaaccttcc ctgcgccggt
     5341 gtatgccaaa tgggctgaca cttaccagaa agaaaccggt aataaagtta actaccaggg
     5401 tatcggttct tccggtggcg taaaacagat tatcgctaat accgttgatt ttggtgcctc
     5461 tgacgcgccg ctgtctgacg aaaaactggc tcaggaaggt ctgttccagt tcccgaccgt
     5521 gattggcggc gtggtgctgg cggttaacat tccagggctg aagtctggcg aactggtgct
     5581 ggatggtaaa accctcggcg acatctacct gggcaaaatc aagaagtggg atgatgaagc
     5641 catcgccaaa ctgaatccgg gtctgaaact gccttcacaa aacattgctg tagtacgccg
     5701 cgcagatggc tccgggactt ccttcgtctt caccagctac ctggcgaaag tgaacgaaga
     5761 gtggaaaaac aacgttggta ctggctctac cgtaaaatgg ccgatcggtc tgggcggtaa
     5821 aggtaacgac ggtatcgccg cgttcgttca gcgtctgccg ggtgcaattg gttatgttga
     5881 atatgcttac gcgaagcaga acaacctggc gtacaccaaa ctgatctccg ctgatggtaa
     5941 accggttagt ccgaccgaag aaaacttcgc taatgcagca aaaggtgcag actggagcaa
     6001 aaccttcgct caggatctga ccaaccagaa aggcgaagat gcatggccta ttacctctac
     6061 cacgttcatt ctgatccaca aagatcagaa gaaaccagaa caaggcacag aagtgctgaa
     6121 attcttcgac tgggcgtaca aaaccggggc taaacaggcg aacgacctgg attacgccag
     6181 cctgccggat agtgtagttg aacaggttcg cgctgcgtgg aagaccaata ttaaagacag
     6241 tagcggtaag ccgctgtact aataaaactc caggccgggt acggtgtttt acgccgcatc
     6301 cggcattaca aaatgacttt gtaaacgcgt ttaactgaag agtaacttat ggctgcaacc
     6361 aagcctgctt ttaacccacc gggtaaaaag ggcgacataa ttttcagcgt gctggtaaaa
     6421 ctggcggcgc tgattgtgct attgatgttg ggtggcatta ttgtctctct gatcatctcc
     6481 tcctggccga gcattcagaa atttggtctg gctttcctat ggaccaaaga gtgggatgca
     6541 ccgaacgata tctacggggc gctggtgccg atctacggta cgttggtgac ttcgtttatc
     6601 gcgctgctga tcgccgtccc ggtgagtttc ggtatcgccc tgttcctgac tgagcttgcg
     6661 cctggctggc tgaaacgccc gctgggtatc gccattgagc tgctggcagc cattccaagt
     6721 atcgtttacg gcatgtgggg cctgtttatc tttgcgccgc tgttcgccgt ttactttcag
     6781 gagccggtcg gcaatatcat gtcgaatatc ccgattgttg gcgcgctgtt ctctggcccc
     6841 gcatttggta tcggtatcct cgcggcaggc gtgatcctcg ccatcatgat tattccgtac
     6901 attgcggcgg taatgcgtga tgtgttcgaa caaaccccgg tgatgatgaa agagtcggcc
     6961 tacggtattg gctgcaccac ctgggaagtt atctggcgta tcgttcttcc gttcaccaaa
     7021 aatggtgtta tcggcggcat catgctgggg ctgggccgcg cgctcggtga aaccatggcg
     7081 gtgaccttta tcatcggtaa cacctaccag ctcgacagcg cctcgctgta tatgccgggc
     7141 aacagtatca cctctgcgct ggcgaacgaa tttgcggaag cggaatccgg tctgcacgtt
     7201 gccgcactga tggaactggg cctgatcctg tttgtgatta ccttcatcgt cctcgccgca
     7261 tcgaagttta tgattatgcg cctggctaag aatgaggggg cacgctaatg gctatggttg
     7321 aaatgcaaac cactgcggcg ctggctgaat ctcgccgcaa aatgcaggcg cgtcgccgcc
     7381 tcaaaaaccg tattgcgctg acgctctcga tggcgacgat ggccttcggc ctgttctggc
     7441 tgatctggat tttaatgtcc accatcactc gcggtatcga cggtatgtcg ctggcgctgt
     7501 tcactgaaat gacgccgccg cccaatacgg aaggtggtgg tctggcgaac gccctggcgg
     7561 gtagcgggct gttaattttg tgggccacgg tattcggtac gccgctgggc attatggcgg
     7621 ggatttatct ggcggaatat ggtcgtaaat cctggctggc agaagtgatt cgcttcatta
     7681 acgacattct gctctccgcg ccgtcgattg tggttggtct gtttgtttac accatcgtgg
     7741 tggcgcagat ggagcacttc tctggctggg cgggtgtaat tgcgctggcg ctgttgcagg
     7801 tgccgattgt tatccgcacc accgagaaca tgctgaaact ggtgccggac agcctgcgtg
     7861 aagcggctta tgcgctgggt acaccgaagt ggaaaatgat ctccgcgatt acgctgaaag
     7921 cgtcggtgtc cgggatcatg accggtatcc tgctggcgat tgcccgtatt gccggtgaaa
     7981 ccgcgccgct gctgtttacc gcgctctcca accagttctg gagcacggac atgatgcagc
     8041 cgatcgccaa cctgccggtg acgatcttta agtttgcgat gagcccgttt gcagaatggc
     8101 agcaactggc ctgggccggg gtgttgatta ttaccctgtg cgtactgctg ctgaacattc
     8161 tggcgcgcgt tgtttttgcg aagaataaac acggttaaca ctttgccgga tgcggcgtga
     8221 acgccttatc cggcctacgg taagcctgat tcgcgaagtg catcaggcac gatgaggaaa
     8281 agattgcaat gagtatggtt gaaactgccc cgagtaaaat tcaggttcgt aatttgaact
     8341 tctactacgg caaattccat gccctgaaaa acatcaacct ggatatcgct aaaaaccagg
     8401 ttacggcgtt tatcgggccg tccggctgcg gtaaatcgac gctgctgcgt accttcaaca
     8461 aaatgtttga actgtacccg gagcagcgtg cggaaggtga aattctgctt gatggcgaca
     8521 acatcctgac caactctcag gatatcgccc tgctgcgtgc gaaagtgggc atggtgttcc
     8581 agaaaccgac gccgttcccg atgtccatct acgacaacat cgcctttggc gttcgtctgt
     8641 ttgagaagct ctcccgggcc gacatggatg agcgcgtgca gtgggcattg accaaagccg
     8701 cattgtggaa cgaaaccaaa gataaattgc accagagcgg ttactctctc tctggtggtc
     8761 agcaacagcg tctgtgtatt gcgcgtggta tcgccattcg cccggaagtg ctgctgctcg
     8821 acgaaccgtg ttcggcgctc gaccccatct ctaccgggcg tattgaagag ctgatcaccg
     8881 aactgaagca ggattacacc gtggtgatcg tcacccacaa catgcagcag gctgcgcgtt
     8941 gttccgacca cacggcgttt atgtacctgg gcgaattgat tgagttcagc aacacggacg
     9001 atctgttcac caagccagcg aagaaacaaa cagaagacta catcaccggt cgttacggtt
     9061 gattcaggag tgcgttatgg acagtctcaa tcttaataaa catatttccg gccagttcaa
     9121 cgccgaactg gaaagtatcc gcacgcaggt gatgaccatg ggcggcatgg tggagcagca
     9181 gctttctgat gcaatcaccg cgatgcataa ccaggacagc gatctggcga agcgcgtgat
     9241 cgaaggcgac aagaacgtca acatgatgga agtggcgatc gatgaagcct gcgtgcgcat
     9301 tattgccaaa cgtcagccga cggcgagcga cctgcgtctg gttatggtga tcagtaaaac
     9361 cattgccgag ctggagcgta ttggtgacgt ggcggacaaa atctgccgta ctgcgctgga
     9421 gaaattctcc cagcagcatc agccgttgct ggtaagtctg gagtcgctgg gccgtcatac
     9481 catccagatg ctgcacgacg tgctggacgc gttcgcgcgg atggacattg acgaagcggt
     9541 acgtatttat cgtgaagata aaaaagtcga tcaggaatac gaaggtattg ttcgtcaact
     9601 gatgacctac atgatggaag attcgcgtac cattccgagc gtacttactg cgctgttctg
     9661 cgcgcgttct atcgaacgta ttggcgaccg ctgccagaat atttgtgagt ttatcttcta
     9721 ctacgtgaag gggcaggatt tccgtcatgt aggcggtgat gagctggata aactgctggc
     9781 gggaaaagat agcgacaaat aattcaccag acaaatccca ataacttaa
//
LOCUS       DAMZII010000170         9618 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZII010000170 DAMZII010000000
VERSION     DAMZII010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.170"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            32..1006
                     /gene="cysB"
                     /locus_tag="POA77_004245"
     CDS             32..1006
                     /gene="cysB"
                     /locus_tag="POA77_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDI6367587.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1301..1465
                     /locus_tag="POA77_004246"
     CDS             1301..1465
                     /locus_tag="POA77_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDI6367588.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1468..1635
                     /locus_tag="POA77_004247"
     CDS             1468..1635
                     /locus_tag="POA77_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367589.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1749..1844
                     /locus_tag="POA77_004248"
     CDS             1749..1844
                     /locus_tag="POA77_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367590.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2008..4683
                     /gene="acnA"
                     /locus_tag="POA77_004249"
     CDS             2008..4683
                     /gene="acnA"
                     /locus_tag="POA77_004249"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI6367591.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4747..5337)
                     /gene="ribA"
                     /locus_tag="POA77_004250"
     CDS             complement(4747..5337)
                     /gene="ribA"
                     /locus_tag="POA77_004250"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDI6367592.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5507..6271
                     /gene="pgpB"
                     /locus_tag="POA77_004251"
     CDS             5507..6271
                     /gene="pgpB"
                     /locus_tag="POA77_004251"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDI6367593.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6420..6728
                     /gene="lapA"
                     /locus_tag="POA77_004252"
     CDS             6420..6728
                     /gene="lapA"
                     /locus_tag="POA77_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDI6367594.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6735..7904
                     /gene="lapB"
                     /locus_tag="POA77_004253"
     CDS             6735..7904
                     /gene="lapB"
                     /locus_tag="POA77_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDI6367595.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8097..8834
                     /gene="pyrF"
                     /locus_tag="POA77_004254"
     CDS             8097..8834
                     /gene="pyrF"
                     /locus_tag="POA77_004254"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI6367596.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8834..9160
                     /gene="yciH"
                     /locus_tag="POA77_004255"
     CDS             8834..9160
                     /gene="yciH"
                     /locus_tag="POA77_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDI6367597.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9286..9504)
                     /gene="osmB"
                     /locus_tag="POA77_004256"
     CDS             complement(9286..9504)
                     /gene="osmB"
                     /locus_tag="POA77_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDI6367598.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2387 a         2259 c         2501 g         2471 t
ORIGIN      
        1 gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt
       61 tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt acacatcaca
      121 acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc aaattttttc
      181 ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc
      241 tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc acacctggcc
      301 ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg cgttaccaaa
      361 cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc accagggctc
      421 gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta tcgccacaga
      481 agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga atcgggctat
      541 tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg aagaactggc
      601 gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc
      661 ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat
      721 taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc
      781 ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc acagtacaac
      841 caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt tcattcagcg
      901 ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga
      961 agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt
     1021 ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta cttatttcta
     1081 taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt aaagtagtgt
     1141 ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca aaagattgaa
     1201 ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt ctttaggatt
     1261 tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa gtgatggcga
     1321 ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc ctgaattaaa
     1381 acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact
     1441 gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga gtcgtcacca
     1501 ttatgccagc atagtgacaa tgaaacagaa acgaaacggg aatgttccgt cgttattcca
     1561 gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc tgaagatgat
     1621 cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt aaagggcttt
     1681 taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa ccgttaaggg
     1741 tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg ttttctctgt
     1801 cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg accccgctgc
     1861 ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt tgggttgtta
     1921 tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag aatcagggct
     1981 tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc cagtaaggac
     2041 acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc tgctaaatca
     2101 ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc
     2161 tggcaggatg gtaactcggt taccgaagag gatatccacg cgctggcagg atggctgaaa
     2221 aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat gcaggacttt
     2281 accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa acgcctcggc
     2341 ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga ccactcggtg
     2401 accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct ggaaatggag
     2461 cgcaaccacg aacgttatgt gttcctgaaa tggggaaaac aagcgttcag tcggtttagc
     2521 gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct cggcaaagca
     2581 gtgtggagtg aattgcagga cggtgaatgg attgcttatc cggatacact cgttggtact
     2641 gactcgcaca ccactatgat caacggcctt ggcgtgctgg ggtggggcgt gggtgggatc
     2701 gaagcagaag ccgcaatgtt aggccagccg gtttccatgc ttatcccgga cgtagtgggc
     2761 tttaaactta ccggtaaatt acgtgaaggt attaccgcca cagacctggt tctcactgtt
     2821 acccaaatgc tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta tggtgatggt
     2881 ctggattcac taccgctggc ggatcgcgcc accattgcca atatgtcgcc agaatatggt
     2941 gccacctgtg gcttcttccc aatcgatgct gtaaccctcg attacatgcg tttaagcggg
     3001 cgcagcgaag atcaggttga gttggtcgaa aaatatgcca aagcgcaggg catgtggcgt
     3061 aacccgggcg atgaaccgat ttttaccagt acgttagaac tggatatgaa tgacgttgaa
     3121 gcgagcctgg cagggccaaa acgcccacag gatcgcgttg cactgcccga tgtaccaaaa
     3181 gcatttgccg ccagtaacga actggaagtg aatgccacgc ataaagatcg ccagccggtc
     3241 gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt cattgctgcg
     3301 ataacctcgt gcaccaacac ctctaaccca agtgtgctga tggccgcagg cttgctggcg
     3361 aaaaaagccg taactctggg cctcaagcgg caaccgtggg tcaaagcgtc gctggcaccg
     3421 ggttcgaaag tcgtttctga ttatctggca aaagcgaaac tgacaccgta tctcgacgaa
     3481 ctggggttta accttgtggg atacggttgt accacctgta ttggtaactc tgggccgctg
     3541 cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc ggtgctgtcc
     3601 ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaactaactg gctggcctcg
     3661 ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct ggctgctgag
     3721 cctattggtc atgatcgtaa aggcgacccg gtttatctga aagatatctg gccatcggca
     3781 caagaaattg cccgtgcggt agaacaagtc tccacagaaa tgttccgcaa agagtacgca
     3841 gaagtttttg aaggcacagc agagtggaag gaaattaacg tcacacgatc cgatacctac
     3901 ggttggcagg aggactcaac ctatattcgc ttatcgcctt tctttgatga aatgcaggca
     3961 acaccagcac cagtggaaga tattcacggt gcgcggatcc tcgcaatgct gggggattca
     4021 gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc agcgggtcga
     4081 tatctacaag gtcggggtgt tgagcgaaaa gactttaact cctacggttc gcggcgtggt
     4141 aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa tgaaatggtg
     4201 cctggcgttg aaggggggat gacgcggcat ttacctgaca gcgacgtagt ctctatttat
     4261 gatgctgcga tgcgctataa gcaggaacaa acgccgctgg cggtgattgc cgggaaagag
     4321 tatggatcag gctcaagccg tgactgggcg gcaaaaggtc ctcgtctgct tggaattcgt
     4381 gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg catgggcatc
     4441 ctgccgctgg aatttccgca aggcgtaacg cgtaaaacgt taggactaac cggggaagag
     4501 aagattgata ttggcgatct gcaaaatcta caacccggtg cgacggttcc ggtgacgctt
     4561 acgcgcgcgg atggtagcca ggaggtcgta ccctgccgtt gtcgtatcga caccgcgacg
     4621 gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa tatgttgaag
     4681 taacaagtat ttgcttgccg gttattttgc ttccggcaag caaactaatt acacaatgca
     4741 agagggttat ttgttcagca aatggcccat tttctcggct ttggtatcga gataatgttc
     4801 gttattgggg ttacgaccta caatcaatgg tacgcgttca acaatattaa tccctgcttc
     4861 ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat tgacgccaag
     4921 gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga agcctaactg
     4981 gtggttagcc tctacggtat cgtaaccttg atcctgcagt gcgtaagcgc ggattttatt
     5041 cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac ggccttcctc
     5101 ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca agctgaacag
     5161 ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat gcccggaaat
     5221 atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa atcccaccat
     5281 caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa gctgcatgaa
     5341 attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga aggggttcac
     5401 ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat ctgtcaattt ttcctggaac
     5461 tggcgttttc agttatgatt gtgggactta tcaaaaagga gaggccatgc gttcgattgc
     5521 cagacgtacc gcagtgggag ctgcactatt gcttgtcatg ccagtagccg tatggatttc
     5581 tggctggcgt tggcaacctg gagaacaaag ttggctacta aaagcggctt tttgggttac
     5641 tgaaactgtc acccagccct ggggcgtcat tacacatttg attttattcg gctggtttct
     5701 ctggtgtctg cgttttcgca ttaaggctgc ctttgtatta tttgccattc tggcggccgc
     5761 aatccttgtg ggacaaggcg ttaaatcctg gatcaaagac aaagtccagg aaccacgacc
     5821 ttttgttatc tggctggaaa aaacacatca tattccggtt gatgagttct acactttaaa
     5881 gcgagcagaa cgcggaaatc tagtgaaaga acagttggct gaagagaaaa atatcccaca
     5941 atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg gtcacacgat
     6001 gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc ggcgaacgtt
     6061 aaccattgct atcttgctgg tctgggcaac gggagtcatg ggaagccgcc tgctgctcgg
     6121 gatgcattgg ccacgcgatc tggtagtagc tacgttgatt tcgtgggcgc tggtggcggt
     6181 ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca ccacctgcgg aagaaaatcg
     6241 cgaaatagcg caacgagaac aagaaagtta acgctggttg attttccgaa tttagccctt
     6301 aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaaaatg gtactttaaa
     6361 gggctattgc ggtaagttga ccataattta ttcgctctaa ccacataacg ggaagtaatg
     6421 tgaaatattt actcattttc ttactggtgt tagcgatctt cgtgatttcg gtcacgttgg
     6481 gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg gagtaccgta
     6541 tttccacatt gctggcggta ttgtttgctg cggggtttgc tatcggttgg ttgatttgtg
     6601 gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa ataaagcgac
     6661 tggaaaacca gctttcaccc gcgactgacg tggctgtagt gccgcactcg tcagcggcga
     6721 aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc gctgcctatg
     6781 gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc aaccgcttgt
     6841 cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa tcaacaggat aaagcggtag
     6901 acctgtttct cgatatgctt aaagaggata caggcaccgt tgaagcccac cttacgctcg
     6961 gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat cagaccctaa
     7021 tggaaagcgc ctcgctgacc tatgaacagc gtctgttggc gattcaacaa ctagggcgtg
     7081 attacatggc cgccgggtta tacgaccgcg cggaagacat gttcaatcag ctgaccgatg
     7141 aaactgactt ccgcattggc gctctgcaac agttgctaca aatctaccag gctaccagtg
     7201 agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa gataaacagc
     7261 gcgtcgaaat tgcccatttc tactgtgagt tagccctgca gcatatggcc agcgacgatc
     7321 tcgatcgtgc catgaccttg ctaaaaaaag gggcggcggc agataaaaac agcgcccgcg
     7381 tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa gccgtcgaaa
     7441 gtttgcaacg cgtgatatcc caggacagag aactggtcag cgaaacgctg gaaatgttgc
     7501 aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg caacgcgcgg
     7561 tggaagagaa caccggtgcc gatgctgaat tgatgcttgc tgatatcatc gaagcgcgcg
     7621 acggtagtga ggccgcacag gtctatatta cgcgccagct tcagcgtcat ccgaccatgc
     7681 gtgtgttcca taagttaatg gattaccact taaatgaagc ggaagaaggg cgtgccaaag
     7741 agagcctgat ggtgctgcgt gacatggttg gcgaaaaggt acgtagtaag cctcgttatc
     7801 gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg tcttgtcggg
     7861 cctggtcaac cattaaaccg attcgcggtc ttgatggcct gtaattttta aaaaaatcct
     7921 actttagtta caacatacta gttattgatg ttccattgtg ctccggcaac gacggcgcag
     7981 aaaaagcctg ccaggggaaa aatcgcaact gttaattttt tatttccacg ggtagaatgc
     8041 tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt ctggtcatga
     8101 cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt gttgcccttg
     8161 attatcataa tcgtgatgac gcgctgtcct ttgtcgacaa gatcgaccca cgcgattgtc
     8221 gtctgaaggt cggcaaagag atgtttacat tgtttgggcc acagtttgtg cgcgaacttc
     8281 aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatattcct aacactgctg
     8341 cgcacgctgt cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt catgcctctg
     8401 gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt tccgtttggc aaagatgcac
     8461 cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg gtcgatcttg
     8521 gcatgacact gtcacctgca gattatgcag aacgtctggc ggcactgacg caaaaatgtg
     8581 gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag gtattcggtc
     8641 aggagttcaa actggttacg ccgggcattc gtccgcaggg gagtgaagct ggtgaccagc
     8701 gccgcattat gacgccagaa caggcgttgt cggctggtgt tgattatatg gtgattggtc
     8761 gcccggtaac gcaatcggta gatccagcgc agacgctgaa agcgatcaac gcctctttac
     8821 agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacgg aaaccggacg
     8881 tattgatgag cccaaagcgg cccctgtacg ccctaaaggc gacggtgtgg tgcgtattca
     8941 gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg attaccggtg tcgatctcga
     9001 tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct gcggcggagc
     9061 agttaaagat ggagttattg aaatccaggg tgataagcgt gatttattaa aatcactact
     9121 tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa cataaaaagc cacggatata
     9181 tccgtggctt tcgaatattt tactgtgcgt attattcagt tatttcgtac tgaacagatt
     9241 caagcgtatt ataattgcga gtcgataatc gacatttatt tacgattatt taccgacctg
     9301 gtgaccaata acaccaccga cagctgcacc acctaatgta cccaacgtac tgccatcggt
     9361 cagtactgca ccgcctaatg cccctgcacc cgcgccgatt gcggtgttgc ggtcccgttt
     9421 agaccagtta gaacaggcac tcagagacat tgccaaagta attgccagaa cagccgcggt
     9481 catttttttg ctcgttacaa acataatact ctctcctgaa tttatgattc acggaagtaa
     9541 gctctctata actataatag ataagaataa gcctggtgaa attattccgt gaaatctgct
     9601 cgcgcgcagg gttatatc
//
LOCUS       DAMZII010000171         9512 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZII010000171 DAMZII010000000
VERSION     DAMZII010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.171"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..1087
                     /locus_tag="POA77_004257"
                     /pseudo
     CDS             1..1087
                     /locus_tag="POA77_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1423..2400
                     /gene="csiD"
                     /locus_tag="POA77_004258"
     CDS             1423..2400
                     /gene="csiD"
                     /locus_tag="POA77_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDI6367599.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2420..3688
                     /gene="lhgO"
                     /locus_tag="POA77_004259"
     CDS             2420..3688
                     /gene="lhgO"
                     /locus_tag="POA77_004259"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDI6367600.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3711..5159
                     /gene="gabD"
                     /locus_tag="POA77_004260"
     CDS             3711..5159
                     /gene="gabD"
                     /locus_tag="POA77_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI6367601.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKLL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5173..6453
                     /gene="gabT"
                     /locus_tag="POA77_004261"
     CDS             5173..6453
                     /gene="gabT"
                     /locus_tag="POA77_004261"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDI6367602.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAGIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6764..8164
                     /gene="gabP"
                     /locus_tag="POA77_004262"
     CDS             6764..8164
                     /gene="gabP"
                     /locus_tag="POA77_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDI6367603.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8185..8847
                     /gene="csiR"
                     /locus_tag="POA77_004263"
     CDS             8185..8847
                     /gene="csiR"
                     /locus_tag="POA77_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDI6367604.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8848..9297)
                     /gene="kbp"
                     /locus_tag="POA77_004264"
     CDS             complement(8848..9297)
                     /gene="kbp"
                     /locus_tag="POA77_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDI6367605.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9381..>9512)
                     /locus_tag="POA77_004265"
     CDS             complement(9381..>9512)
                     /locus_tag="POA77_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDI6367606.1"
                     /translation="IILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQTRD"
BASE COUNT         2275 a         2407 c         2566 g         2264 t
ORIGIN      
        1 atgggaaaac ctccatgcat taaaagaaaa gcgtttaaat ggaatgccta aaacaacacc
       61 caaaagtgac caggttatta tgttgcaaaa tcaatacgtt cgtgaaatgc gaaaatatgg
      121 cgtacgaggt ttacgttatg atgcggcaaa acactcaaaa catgaacaaa tagaaagatc
      181 aataacccca ccgcttaaaa attataatga gcggttacac aatactaact tatttaaccc
      241 aaaatatcat aaaaaagccg ttatgaatta catggaatat ctggtaactt gtcagttgga
      301 tgaacaacaa atgtcatcac tgctttatga aagagatgat ttaagcgcca ttgatttttc
      361 attgctcatg aaaacgataa aagccttttc atttggtgga gatcttcaaa cccttgcatc
      421 aaaaccgggt tccacaatct caagtatccc atcagaaaga cggatattga ttaacattaa
      481 ccacgatttt cctaacaatg gtaatctttt caatgacttt ctatttaacc atcaacaaga
      541 tgaacaatta gcaatggcat atatagccgc tctgccgttc agcaggcctt tagtttactg
      601 ggatggccaa gtattaaaat caacgactga aattaaaaat tatgatgggt ccacgcgtgt
      661 cggcggtgag gcgtagctta ataaaggttg ctctacctat cagcagctct acaatgaatt
      721 ccacgcatta tatatagata aagcaggaat atggagcgca tttgagggtg tatctgcaac
      781 taaaaacgtt ctggccttta gtcgtgggga ttctgtgaac attaatcact ctcctcatga
      841 tggactagtt ataataaata aaggaaacga agaagttgaa ggtacctggc ctaacaaatt
      901 gcaacctgga atatacaaaa acatggggag taatagcgtt aacattatta ttaataatac
      961 ccgaaaaatt atcccccctg gtaaagtatt tacgcttaga ggcggaactc taaatatcaa
     1021 tattcctggg cgtagcgctc ttcttttagg gaaaacagga gaaccgccga actatctcta
     1081 tttataattt aatgttatat ctgccccgat aaaacggggc agataatatg tttagtttac
     1141 taacggtcat tttgcagtga agccatttac tgttttttat cgaccagata atctgttctc
     1201 taatgttaac tccccctaac ctgttgcttt agttattcat ttcctgtctc actttgcctt
     1261 aataccctac gttaaatgtt actaatttgt tgcttttgat cacaataaga aaacaatatg
     1321 tcgcttttgt gcgcattttt cagaaatgta gatattttta gattatggct acgaaatgag
     1381 catcgccatg tcaccctaca tctcataaga ggatcgcttc tgatgaatgc actgaccgcc
     1441 gtacacaata acgctgtcga ttcaggccag gactatagcg gattcactct catcccgtcg
     1501 gcgcaatccc cgcgtctgct ggaactcacc ttcaccgaac agacgaccaa acagtttctc
     1561 gaacaggttg ccgaatggcc cgtgcaggcg ctggagtaca aatcgtttct gcgttttcgg
     1621 gtaggcaaaa ttcttgacga tctgtgtgcg aatcagctgc aaccattgct gttgaagacc
     1681 ctgctaaacc gtgctgaagg tgcgctgttg atcaatgcgg tgggtatcga tgatgtggcg
     1741 caggcagatg agatggtgaa gctggcaacg gcggtagcgc atctgattgg tcgctcaaat
     1801 ttcgatgcga tgagcggtca gtattacgcg cgtttcgtgg tgaaaaatgt cgataactca
     1861 gacagctatc tgcgtcagcc gcaccgcgtg atggaactgc acaacgacgg cacgtatgtc
     1921 gaagagatca ccgattacgt gctgatgatg aaaatcgatg agcaaaacat gcagggtgga
     1981 aattcgttgc tgctgcatct cgatgactgg gaacatctgg accactattt ccgccatcca
     2041 ctggcgcgtc gcccgatgcg ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt
     2101 ttccatccag tgttcgacgt tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag
     2161 ttcgtccagc caaaagattt tgaagaaggc gtgtggttga gcgagctttc cgatgccatt
     2221 gaaaccagca aaggcattct ttctgtaccc gttcccgttg gcaaattcct gttgataaac
     2281 aacctgttct ggctgcacgg tcgcgaccgc tttactccgc acccggatct gcgccgtgaa
     2341 ctgatgcgtc agcgtggcta tttcgcttac gccactcacc actaccagac gcatcagtaa
     2401 gcgcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat
     2461 gtcgaccgcc atgcaactga ttgatgttta tccggatgcc cgcattgcgt tgctggaaaa
     2521 agagtccggc ccggcctgtc accagacggg ccacaacagc ggcgtgatcc atgccggggt
     2581 ctattacacg cccggcagcc tgaaggcgca gttttgcctg gcgggaaacc gcgccaccaa
     2641 agccttttgc gatcaaaacg acattcgcta tgacaactgc ggcaagatgc tggtcgccac
     2701 gtccgatctc gaaatggaac ggatgcgcgc gttgtgggaa cgcacggcgg cgaacggtat
     2761 cgagcgcgag tggttaaacg ccgaggaact gcgcgagcgc gaaccgaata tcaccgggct
     2821 cggcggcatt tttgtgccgt ccagcggcat tgtcagctat cgcgaagtca cagcggcaat
     2881 ggcaaaaatc ttccaggcca gaggcggtga gattatttat aacgccgaag tcagcgccct
     2941 cagtgagcat aaaaacggcg tggtcgtccg aacccgtcag ggcggcgaat atgaagcatc
     3001 gacgctgatt agctgttccg ggctgatggc tgaccggctg gtgaaaatgc tcggcctcga
     3061 accgggtttt attatctgcc cgttccgcgg cgagtatttc cgccttgcgc cggagcataa
     3121 ccagattgtt aaccatctga tttaccccat tcccgacccg gcaatgccat ttttgggcgt
     3181 gcatctcacc cgaatgatcg atggcagcgt caccgtcggg ccaaacgcgg tgctggcttt
     3241 caaacgcgaa ggctatcgca agcgcgactt ctcatttagc gacacgctgg agattttggg
     3301 ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa
     3361 aaactcgctg tgcaaaagcg gctatctgcg cctggtgcaa aagtattgtc cccggctttc
     3421 gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg caggcagtat cgccggacgg
     3481 caagctgatt gacgattttc tgtttgtcac caccccacgc acgatccaca cctgcaatgc
     3541 gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca
     3601 aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga
     3661 tgccttacac gccgcattta atcaataacc tttgaaaaca ggacgtaaca atgaaactta
     3721 acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca
     3781 atggtgaagt catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga
     3841 aaatgggcgc tgatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcct
     3901 ggcgtgcgct caccgccaaa gaacgcgcca acattctgcg caactggttc aatttgatga
     3961 tggagcatca ggacgattta gcgcgcctga tgaccctcga acagggcaaa ctactggccg
     4021 aagcgaaagg tgaaatcagc tacgccgcct cttttattga gtggtttgcc gaagaaggca
     4081 aacgcattta tggcgacacc attcccggtc atcaggccga taagcgcctg attgttatca
     4141 agcagccgat tggcgttacc gcagctatca cgccgtggaa cttcccggcg gcgatgatta
     4201 cccgcaaagc cggtccggcg ctggcggcag gctgcaccat ggtgctgaaa cccgccagtc
     4261 agacgccgtt ctctgcgctg gcgctggcgg agctggcgat tcgcgcgggc attccggctg
     4321 gggtatttaa cgtggtcacc ggttcggcgg gcgcagtcgg taacgaactg accagcaacc
     4381 cgctggtgcg caaactgtcg tttaccggtt cgaccgaaat tggccgccag ttaatggaac
     4441 agtgcgcaaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg
     4501 tctttgacga tgccgatctc gataaagccg tggaaggtgc gctgtcctcg aaattccgca
     4561 acgccgggca aacctgcgtc tgcgccaacc gtttatacgt gcaggacggc gtgtatgacc
     4621 gctttgccga aaaattacag caggcggtga gcaaactgca catcggcgac gggctggaga
     4681 atggcgtcac catcgggccg ctgatcgatg aaaaagcggt agcaaaagtg gaagagcata
     4741 ttgccgatgc gctggagaaa ggtgcgcgtg tggtttgcgg cggtaaagcg gatgaacgcg
     4801 gcggcaactt cttccagccg accattctgg tggacgtgcc ggccaacgcc aaagtgtcga
     4861 aagaagagac gttcggcccc ctcgccccgc tgttccgctt taaagatgaa gccgatgtga
     4921 ttgcgcaagc caatgacacc gagtttggcc ttgccgccta tttctacgcc cgtgatttaa
     4981 gccgcgtctt ccgcgtgggc gaagcgctgg agtacggcat cgtcggcatc aataccggca
     5041 ttatttccaa tgaagtggcc ccgttcggcg gcatcaaagc ctcgggtctg ggtcgtgaag
     5101 gttcgaagta tggcatcgaa gattacttag aaatcaaata tatgtgcatc ggtctttaac
     5161 tggagaatgc gaatgaacag caataaagag ttaatgcagc gccgcagtca ggcgattccc
     5221 cgtggcgttg ggcaaattca cccgattttc gctgaccgcg cggaaaactg ccgggtgtgg
     5281 gacgttgaag gccgtgagta tcttgatttc gcgggcggga ttgcggtgct caataccggg
     5341 cacctgcatc cgaaggtggt ggccgcggtg gaagcgcagt tgaaaaaact gtcgcacacc
     5401 tgcttccagg tgctggctta cgagccgtat ctggagctgt gcgagattat gaatcagaag
     5461 gtgccgggcg atttcgccaa gaaaacgctg ctggttacga ccggttccga agcggtggaa
     5521 aacgcggtaa aaatcgcccg cgccgccacc aaacgtagcg gcaccatcgc ttttagcggc
     5581 gcgtatcacg ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa tccgtactct
     5641 gcgggcatgg ggctgatgcc gggtcatgtt tatcgcgcgc tttatccttg cccgctgcac
     5701 ggcataagcg aggatgacgc tatcgccagc atccaccgga tcttcaaaaa tgatgccgcg
     5761 ccggaagata tcgccgccat cgtgattgag ccggttcagg gcgaaggcgg tttctacgcc
     5821 gcgtcgccag cctttatgca gcgtttacgc gcgctgtgtg acgagcacgg gatcatgctg
     5881 attgccgatg aagtgcagag cggcgcgggg cgtaccggca cgctgtttgc gatggagcag
     5941 atgggcgttg cgccggatct cactaccttt gcgaaatcga tcgcgggcgg cttcccgctg
     6001 gcgggcgtca ccgggcgcgc ggaagtgatg gatgccgtcg ctccaggcgg tctgggcggc
     6061 acctatgccg gtaacccgat tgcctgcgtg gcggcgctgg aagtgttgaa ggtgtttgag
     6121 caggaaaatc tgctgcaaaa agccaacgat ctggggcaga agctaaaaga cggattgttg
     6181 gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg ggctgggagc gatgattgcc
     6241 atcgagctgt ttgaagatgg cgatcacagc aaaccggacg caaaactcac cgccgggatc
     6301 gtggcacgcg cccgcgataa gggcctgatt cttctctcct gcggcccgta ttacaacgtg
     6361 ctgcgcatcc ttgtaccgct caccattgaa gacgctcaga tccgtcaggg tctggagatc
     6421 atcagccagt gttttgctga ggcgaagcag tagctatgtc cgtttgtaag agttaaacgt
     6481 tgctaaggga atgataacga tgcagtaaaa aataataacg atgaggcgat cgacaaagtt
     6541 aaaaacgact gcggactgtc ccctcgcccc tctggggaga gggttagggt gaggggtaat
     6601 cacgcggcta caccctcacc ccggccctct ccctaaggga gagggagaaa accctcccgc
     6661 tttcgttttt aagattttgt tgaccgtctc aattaaacgc gtgacccgac gcgggaaatg
     6721 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
     6781 accacatgag ttaggcggcg ggctgaagtc acgccacgtc accatgttgt ctattgccgg
     6841 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
     6901 ggtattactg gcctatctgt tcgccggatt actggtggtt atgattatgc gaatgctggc
     6961 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagccat
     7021 tggccgctgg gcgggttata ccatcggctg gctgtactgg tggttttggg tactggttat
     7081 cccgctggaa gccaacatcg ccgctatgat cctgcactcg tgggttccag gcattcccat
     7141 ctggttattt tccctcgtca ttaccctcgc cttaactggc agtaatttat taagcgttaa
     7201 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
     7261 tattttcctt ggtgcagtcg caattagcgg tttttaccct tatgccgaag tgagcgggat
     7321 ctcaagattg tgggatagcg gcggctttat gcccaacggc ttcggtgcgg tattaagcgc
     7381 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
     7441 atccgacacg ccggaaaaac atattgtccg cgctaccaac tcggttatct ggcgtatttc
     7501 tatcttctat ttgtgctcta tttttgtcgt agtggcgtta ataccgtgga atatgccggg
     7561 gctgaaagcc gttggttctt atcgctcggt tctggaattg ctcaatattc cccatgcgaa
     7621 attaatcatg gactgcgtga tattactttc cgtaaccagc tgtctgaact cggcgctgta
     7681 taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgcgcccg cggtaatggg
     7741 caaaatcaac cgcagtaaaa ccccgtatgt ggcggtgtta ctctccaccg gagcggcatt
     7801 tttaacggtg gtggtgaact attacgcacc tgcgaaagtg tttaaattcc tgatagacag
     7861 ctccggtgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     7921 taaaattctg cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt acccgtggct
     7981 cacctggctg gtaataggct ttattacctt tgtgttggtg gtgatgttat tccgcccggc
     8041 gcaacagtta gaagtgatct ctaccggctt attagcgata gggattatct gtaccgtgcc
     8101 gattatggcg cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg
     8161 ctgataatga tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg
     8221 aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacgaat gagtttgctg
     8281 acatcgcgtt atgcacttgg cgttgggccg ttacgggaag ctctttcgca actggtggcg
     8341 gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag
     8401 gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgctggt cagtctggcg
     8461 attgcccgcg gtggcgatga atgggaggca gacgttctcg caaaagcgca tctgctgagt
     8521 aagctggagg cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag
     8581 gcgtttcata cagcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa
     8641 cggttgtttg atctggcggc gcgctatcga tttatctggc tgcggcgaac ggtgctttcg
     8701 gtagaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg
     8761 cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt
     8821 atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca cttgccccgg
     8881 ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag
     8941 attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt
     9001 ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat
     9061 accggaaata ttccccaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc
     9121 accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc actttatcgg catcaggtat
     9181 accggttttg ctcagatgct cctgcacctt cttcgcctga tcgtctttat cgtgctgacc
     9241 tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc
     9301 tacctcattc gttaacggaa acactcagaa agtgtagcag aggaacgcgg cgcgttaacg
     9361 ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg
     9421 gggatatacc caagcagtgt cagcagaata ttaataatga acgcccaacc gaacccttta
     9481 ccgagcagca cgccgagagg cggcagaatg at
//
LOCUS       DAMZII010000172         9394 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZII010000172 DAMZII010000000
VERSION     DAMZII010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.172"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1037)
                     /gene="trhU"
                     /locus_tag="POA77_004266"
     CDS             complement(<1..1037)
                     /gene="trhU"
                     /locus_tag="POA77_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhU"
                     /protein_id="HDI6367607.1"
                     /translation="MKKLKKLLLEILIAVMLSLSLPAMTMAADTGVPGAMCQSAGVWQ
                     GLIKNICWSCIFPMRIMGIGAAPEGAAPSRPGCYCTDQNGIPEIGWQLSFFQPVKIVE
                     VVKSPWCSPFLEGTMLQKSQFDIGKSNTNQPMTATEAGFYDVHLWEFPIMTMLKLLVI
                     GECTAEPYVDASLTYISEVDPMWESDLLTLVLNPEAVVFANPIASMVCAADCVAVTAG
                     KDNLAAYFCAGCDGNLYPLTGHIYANDDAVRTSSLITQRLLTKLHRQGMLMRTMGADA
                     MCEKTWEYFTPRSQYRLSMLYPTPEANGPDCCHRLGDSVHDWSTLKGGRKKIGIDNYV
                     YMLWRYNDCCV"
     gene            complement(1182..2690)
                     /gene="trhW"
                     /locus_tag="POA77_004267"
     CDS             complement(1182..2690)
                     /gene="trhW"
                     /locus_tag="POA77_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhW"
                     /protein_id="HDI6367608.1"
                     /translation="MRSFELKTTIKPLASCLAFYALISTAVASPQYQQVADDIARQAQ
                     VLGAQIPVPHASEGPLPSGSLDSPDTKKYIRQAEAMKKNGDLSQQTNRGYVPGMNADS
                     VQAVIDHTQAIRAQSNNSEAVNDIIRRRDEIQGNSQLSESALKSVENKPEVMRSQSSN
                     IEKMFGSSGITAADFERKIDSSREEVLSTENGITIFASFSLPDYVLEDLLRTASEHKA
                     RVVFNGLKKGTTRLPETQAAINQLVVKGKFDSPLITIDPDAFNQYQITQVPTIISREQ
                     SRFAKMVGSFNVDFFQRELARKPDQDLFPIAGTTYPVEEKSIIKELEERAQKYDWDGA
                     KKRAVADTWKNQYMVNLPPAQEHKEWLIDPTIRVTQDVKDKQGRVIASAGELINPLAR
                     FPQNLTMIIFDPMNPGQLEWAEKQYRQRLGSGQVMPMFTRIKQENGWDHLNDLREKFN
                     GKVFKVNEQIIARFQIKNTPALISTEKEKFRITQFSEAEIRGIGTKIAAEEN"
     gene            complement(2677..3189)
                     /gene="trhF"
                     /locus_tag="POA77_004268"
     CDS             complement(2677..3189)
                     /gene="trhF"
                     /locus_tag="POA77_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhF"
                     /protein_id="HDI6367609.1"
                     /translation="MREYIPKKRGLLSFDWYVPINLGILILVMLIFFNRFTFGHGLLN
                     GCLPADFYMIDLKDKSVRSGNIIAFHMPKSVRFIRENEKVIKIVAGVGGDRLRVTLDG
                     VYNGNKFYKANARRISVKYGIPAETIERELTIPEGEVFLIGQTDHSWDSRFWGPVKLT
                     SVIGKTYAIF"
     gene            complement(3463..7761)
                     /gene="rsp"
                     /locus_tag="POA77_004269"
     CDS             complement(3463..7761)
                     /gene="rsp"
                     /locus_tag="POA77_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein RSP"
                     /protein_id="HDI6367610.1"
                     /translation="MRKSGLCLTLLFSLIASIKSVQAEAIMISGKLQADMPAVTFNPG
                     PGDFVAFVNNNTITASGTGNACNVTVDDRATSSVDNLVCFFEWLPNTSGFTANGFNLT
                     GIPNAAGELKLPYKISYFSGSERQKVEVVNGEYTVNALVPVKPTITGLKSSMNGVVQD
                     GYTLKSYLKDEAIRNVTVSVEPRNYVQYISIGSGSACEVAIGGNGCTIDVGSIKVSDS
                     DLLMGSREITVTANSKNNYFAPPEAQKLTLTWDYRPPVVDHTLWNFTDEAKTISVGGQ
                     EIYTGARTVAVAVKVPQEETEGEWWLPTAMTLTMNPDGVFKPTTKVTLEDGTVIDFKQ
                     SWATPLRRTLQPVSGPQKVGDEYLYIFDLTDLTNGSYAASFTVENNSKNTSTYNEPES
                     KLMLSDNPTLMVLKDGAALAKRAPVYFLNEIIVAAFQGQAGVADIKAVTIDNKLVELT
                     PTNHKGIYYLPVGDDLEVNADHEITVIAENLYGKIVTFNTTFTYQPTGFTLKNFEKDI
                     TLYSRVRQYTDLMSQTAGDKCTLFTTEENALAYLEWYGDKSDITACYPQWNNVPDGLE
                     FYFKGRTPGLTGFFNKTGDNLLDYQVYMINGKGSKAVSSRNRHVLITQLPYNPVLTYK
                     KNKVLTGINDNTALAYTTGGEAARLLAKVVPADVTVYVSQNGGESVKTTFKNRSSNND
                     ATTFVQRLKVASAPLWTKNVFDIAVEYSKEPTMRTVDTLNVYTVPDFNIRAAMEIEDK
                     KTATTMELPLKVSVGRYNNSTRKSSFSRTTMGDWDVTIYAQKSVYGKDPETGRYKTTY
                     ERTPLTEALPVNDAGVVETKIKIENMDLGNVRLVGIAKVRSPFADFELTRETSAVSIR
                     VYKGEELEGNLSKSLIIGRIPLSTLVNFKSASTANSDALAPTEWQESSDNGQTWTMLQ
                     DMTGKRSISIRKTEVGKWLYRAKMTNKFTSKESYSDVLTVVTYKQPKLSIDVAEILEG
                     DDLPVTLLDNDEPIPQGTAEVMWSEDKVNWVQGDTTYNVSAADTLPPTIYARMRYLDS
                     DELAEESSWKETSARLTVAKPKRLSVSVSGISSVEVGSKVTLDGKYTNPNSRFKNGND
                     VIEEWTAPDGQKIRGANLSITLTEQMLDKQGYAAFEYSAWLADSKDSTISTRKVSVKS
                     WVYKFPEMKVSTKLKYTMAPSTLHVALSGIKDGGYPGVTYSREWIYDKDNISVLKDDG
                     DTKEFAIAKPGKYTLVIVFRDNRNNEQRIENTFVVDEQTPMTVEMTPKFSNKFMRAPL
                     DVTLRSNIKLAHSADSIDTVTYKVNGEVVPGSKNYWAQLISGLTEKKYEVTLDVVSKM
                     GQRGTASVDFEVVKNAAPQCALSYTESNMSWSFTNKCIDTDGKLVRYEWYINDELRNV
                     FGSTATLSKNLNRGKQDIRVVAYDDSGDSATQRTTVYGPDEGASKSVDSVETSQ"
     gene            complement(7881..8444)
                     /locus_tag="POA77_004270"
     CDS             complement(7881..8444)
                     /locus_tag="POA77_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDI6367611.1"
                     /translation="MTMSDLSATGAKPQRVRVFSQSVAMAFEVNLDKSRSADNSAFVT
                     ETEKYYLSLQFAPPSDNREYGWNSEGSILMKLSQNEAMALASVFLRIKPTLKFEKRKT
                     THRTHQAYKNINIGPNDRGGLLVSASIVPVKMGTFKPLNYNLPVTQMDCVSTGLFLLG
                     FLTLKMPWVSSESIITALRLSESKSGQ"
     gene            complement(8441..8875)
                     /locus_tag="POA77_004271"
     CDS             complement(8441..8875)
                     /locus_tag="POA77_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDI6367612.1"
                     /translation="MKVNFKKVIPLLMVGLTVAGSYSIFNAKPSKPDLYDFTGKVLKT
                     TSVFQPCDKESTPSLNIQIADNGSVHINGVASKVTFVERVPGNEIAVKCTGLQVKNSR
                     LVHTSSYTMIISEGKGGFVISDLTHTKDNEVMSGTWFFKKRV"
     gene            complement(8865..9317)
                     /locus_tag="POA77_004272"
     CDS             complement(8865..9317)
                     /locus_tag="POA77_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer repressor"
                     /protein_id="HDI6367613.1"
                     /translation="MLSRNSLIHGLRRDQLIEVLSISEFPVVLVENPFIQPKSTGKKP
                     VIFNIDEFHVSIAPISSLKYDWEWAPIDTILIEVVIPPAEADLVSAENDFLRDAGIGH
                     IQCEPGGVSIRRTVTFVGGITADNLLYQLRLMCVSALHLIGEELEDES"
BASE COUNT         2405 a         2285 c         1971 g         2733 t
ORIGIN      
        1 ctgacgcagc aatcattata gcgccacagc atgtaaacat agttatctat gccgattttt
       61 ttccgtccac ctttaagcgt tgaccagtca tggacagaat cgcctaggcg atgacagcaa
      121 tccggaccat tcgcctccgg cgttgggtaa agcattgaaa gtcggtattg agatcgagga
      181 gtgaagtatt cccacgtttt ttcacacatt gcgtcagctc ccattgttct cataagcatt
      241 ccctgcctat gtaacttcgt cagtagacgc tgagttataa gtgagctggt tctgacggca
      301 tcatcattcg catagatgtg tccagttaac ggataaaggt tcccatcaca cccggcacaa
      361 aaatatgcag ccaaattatc cttcccggca gtcacggcca cgcagtcagc tgcacatacc
      421 attgaggcga tagggtttgc aaataccaca gcttcggggt ttagcacgag cgtcaatagg
      481 tcactttccc acatcggatc tacttcgctg atatacgtca gactggcatc aacataaggt
      541 tctgctgtac attcaccaat aaccagcaat ttcaacatcg tcatgattgg gaattcccag
      601 agatgtacgt cgtagaatcc ggcttctgtt gcggtcatag gctggttagt attgctcttg
      661 ccgatgtcga actgtgattt ttgcagcatg gttccctcta aaaatgggct gcaccacgga
      721 cttttcacca cttcaacaat ttttactggt tggaaaaaac ttagctgcca tccgatttct
      781 ggtatcccat tttgatccgt gcaatagcaa ccagggcgtg agggggcggc accttcaggg
      841 gctgctccaa ttcccatgat ccgcatcggg aagatacaac tccagcagat gtttttgatt
      901 aggccctgcc atactcccgc tgactggcac attgctcccg ggacaccggt atctgcggcc
      961 attgtcattg caggcagcga gagcgacagc atgacagcga tcaatatttc caaaagtaac
     1021 ttttttagct tcttcaccgg cacgctcact atttagtaat gaatgttgtc acgctttcca
     1081 tatacgcaac cactttgtta aaacactctc tcatctggtc cgggtaagtt agcgtcagcc
     1141 aagtggccag cagcgcccca aaaataaatc ctattttcat atcaattctc ctctgccgcg
     1201 atttttgttc caataccacg tatttcagct tcactgaatt gcgttatacg gaatttctct
     1261 ttttcagtgg agatcagcgc tggcgtgttt tttatctgga aacgagcaat aatttgctca
     1321 ttaactttga acaccttccc attgaatttt tcgcgcagat cattcaaatg atcccatcca
     1381 ttttcctgtt tgatgcgcgt gaacatcggc atcacctgac cgctgccaag acgctggcga
     1441 tattgcttct ctgcccactc aagttgccct gggttcattg ggtcaaaaat tatcattgtg
     1501 aggttctgcg ggaacctggc cagtgggtta atgagctcgc cagcagaagc aatgacgcga
     1561 ccctgcttat ctttaacgtc ctgagtaacc ctgattgtcg ggtcgattag ccattccttg
     1621 tgctcttgag cgggaggcag attaaccatg tactggtttt tccaggtatc agcgactgct
     1681 ctcttcttgg cgccgtccca gtcgtatttc tgtgctcgct cttcgagctc tttaataatg
     1741 ctcttctctt caaccggata ggtcgtacca gcaatcggga aaagatcttg gtcgggtttt
     1801 ctggccagtt cgcgctggaa gaaatcaacg ttgaaagagc cgaccatttt tgcaaaccgg
     1861 gattgctcac gggagattat tgtcgggacc tgcgtgatct ggtactggtt aaatgcgtcc
     1921 gggtcaatcg taattagtgg ggagtcaaac ttccccttca ccaccagctg gttgatagct
     1981 gcctgagttt cagggaggcg tgtagtgcct ttcttcagac cattaaaaac cactctcgcc
     2041 ttatgttctg acgcggtacg gagtagatct tccaggacgt aatcaggcaa gctaaatgac
     2101 gcaaagatag tgataccgtt ttctgtcgag agcacctctt cccgactgct gtctatttta
     2161 cgctcgaagt cagcggcggt gattcctgat gaaccaaaca tcttttcgat attcgaagac
     2221 tgactgcgca taacttctgg tttattttcg acggatttca gtgcagactc gctgagctgt
     2281 gaatttccct gaatctcatc acgtctgcga atgatgtcgt taactgcttc cgagttatta
     2341 ctctgagcgc ggatagcctg cgtatggtct ataactgcct gcacactgtc agcattcata
     2401 cccgggacat aaccgcgatt tgtttgctga ctgaggtccc cgtttttctt catagcttcg
     2461 gcctggcgga tgtatttctt ggtatcagga gaatccagag aaccagacgg gagcggacct
     2521 tctgatgcgt gtggtaccgg tatttgggcg cccagaacct gagcttgcct tgcaatgtca
     2581 tcagcaacct gttgatactg ggggctagcc acagcggtgg atattaaggc gtaaaatgcc
     2641 agacaggagg ccagaggctt aatagttgtt tttaattcaa aagatcgcat atgttttccc
     2701 aataactgaa gtcaacttaa ccggccccca aaaacgtgag tcccatgagt ggtccgtttg
     2761 cccaattaaa aagacctccc cttccggaat agttaattct ctttcaatgg tctcggcagg
     2821 tatgccgtat ttcacagaaa tacgtctggc attggcttta taaaatttgt tcccattata
     2881 aacaccatcc aatgtgaccc tcaggcgatc tccgccgact cctgccacaa ttttaataac
     2941 cttctcattt tcacgtataa aacggactga ttttggcata tgaaaagcta tgatattccc
     3001 tgaccttacc gatttatcct tcagatcgat catgtaaaaa tcagcaggta aacatccatt
     3061 taatagtcca tgaccaaacg taaatcgatt aaaaaaaatc aacataacca gtatcaggat
     3121 tccgagattt attggcacgt accaatcaaa actcagtaaa ccacgcttct taggtatgta
     3181 ttctctcata acctacttct ctcagaatca gaaagcaaaa tggactgtct tttacattac
     3241 cttaaaacct attatccatg ttttggtact tccttatttc agcaaagcca gctatttctg
     3301 attatttacc gccttttttt ctttcattcc gtctttgatt tgtgattgtc ctggccgctg
     3361 tcttactcat gtaaagtaac tttcccgacg ttaccccatc aaaaagtgaa aaacaaaaaa
     3421 gccgggacat ttctgccccg gctgttattt cattagcttg ccttactgcg aggtttcaac
     3481 tgaatctacg gactttgacg cgccctcatc cggaccataa acggtcgtac gctgagttgc
     3541 tgaatcccca ctatcatcat acgcgacaac tcgtatatct tgcttcccac gattaaggtt
     3601 tttggacagt gtcgcagtgc tgccgaaaac attccttagc tcatcattga tataccattc
     3661 atatcgaacc agcttgccat cagtgtcaat acatttgttt gtgaaactcc agctcatatt
     3721 agactctgtg tagcttaatg cacattgcgg agccgcgttt ttaacaacct caaaatcaac
     3781 tgatgcggta cctctttggc ccattttact gacaacatcc agcgtgactt cgtatttctt
     3841 ctcagtaaga ccagaaatta actgagccca atagttttta ctaccaggaa caacctcgcc
     3901 gtttactttg tatgtcacag tatcaatact gtcagcagag tgcgccagtt taatgttaga
     3961 tcttagcgtg acatcaagag gcgccctcat gaacttatta gagaatttcg gtgtcatttc
     4021 tacagtcatg ggcgtttgct catcgactac gaaggtattt tcaatacgct gttcgttgtt
     4081 acggttatca cggaaaacga ttaccaacgt atatttcccc ggcttcgcaa ttgcaaattc
     4141 tttcgtatca ccatcatctt ttaaaactga gatattgtcc ttatcgtaga tccattcccg
     4201 agaataagtt accccaggat agccaccgtc ttttattccg ctcagcgcaa catgtaaggt
     4261 cgacggggcc atggtgtatt taagctttgt actgactttc atctcaggaa acttatacac
     4321 ccaagattta acagatactt tccgcgtgga aatagtactg tccttactgt cagcaagcca
     4381 tgcactgtat tcaaatgccg catatccttg cttatccagc atctgctcag tcaaagttat
     4441 cgacagattc gcacctctga ttttttgtcc gtcaggggca gtccattctt caatgacgtc
     4501 atttccgttc ttgaatcggc tgttcgggtt agtgtattta ccatctaaag taactttact
     4561 tcccacctcg acgcttgaaa taccacttac tgaaacagat aggcgcttgg gtttagcgac
     4621 ggttaaccgg gcagacgtct ctttccagga tgactcttca gccaactcat cagaatccag
     4681 gtaacgcatt cgggcgtaaa tggtcggagg tagcgtatca gcggccgata cgttataggt
     4741 agtgtcaccc tgaacccaat tcactttatc ctccgaccac atcacttcgg ccgtaccctg
     4801 cggaattggt tcatcgttat ccagcagcgt gacaggcaaa tcatcgcctt cgagaatttc
     4861 agcaacatcg atgctcagct taggctgttt gtacgttaca accgtcagaa cgtcgctata
     4921 agactctttc gacgtaaact tgttggtcat ttttgccctg taaagccatt taccaacctc
     4981 agttttcctg atcgaaatgc ttcgcttccc tgtcatgtcc tgaagcatag tccatgtctg
     5041 tccattatca gaagactcct gccactcggt aggcgccagc gcatcggagt tagctgtact
     5101 ggccgatttg aagttaacaa gcgtactgag cgggatacgt ccgataatca gactctttga
     5161 gagattacct tctagctctt cgcccttata aacacggatg ctaactgcac tggtttcccg
     5221 ggtcagttcg aaatctgcga aaggcgatcg tactttagca atcccgacaa gtcgcacgtt
     5281 cccgaggtcc atattttcta tcttgatctt ggtttcaacc accccggcat cattcacggg
     5341 cagtgcttcg gttaggggtg tacgttcata cgtcgtttta tatcggcctg tttctggatc
     5401 ttttccataa acacttttct gagcgtaaat ggttacatcc cagtccccca ttgttgtccg
     5461 actaaatgaa cttttgcggg ttgaattgtt atagcggcca acagacacct tcaatggcaa
     5521 ttccatcgtg gtagcagtct ttttgtcctc aatttccatt gccgctctaa tgttaaagtc
     5581 gggtaccgta tacacattga gcgtatcaac ggttcgcata gtcggttcct tcgagtactc
     5641 gacagctatg tcgaagacat tcttcgtcca caacggggct gatgccactt taagacgttg
     5701 aacaaacgtc gttgcatcgt tatttgatga acggttctta aatgtcgttt ttacgctttc
     5761 ccccccgttt tgtgaaacat agactgtcac atcagcagga accacttttg ccaatagacg
     5821 agctgcttcc ccgccagtgg tatacgccag tgcggtgtta tcgttaatcc ccgtcaacac
     5881 cttgtttttc ttgtaggtaa ggaccgggtt ataaggtaac tgcgtaatca gcacatggcg
     5941 gtttctggac gatacagctt tagagccctt gccgtttatc atatagacct gatagtcgag
     6001 caggttatcg cctgttttat tgaagaaccc tgtcaggcct ggcgtccgac ctttaaagta
     6061 aaattcaagc ccgtcaggca cattgttcca ctgagggtaa caggcggtga tatcagattt
     6121 gtcaccatac cattcaaggt atgccagtgc gttctcttct gtagtaaaga gagtgcattt
     6181 atcaccggcc gtctgactca tgagatcggt atactgccga actcgtgagt acaatgtgat
     6241 gtctttctca aagtttttaa gcgtgaatcc tgttggttga tacgtgaaag tagtattaaa
     6301 tgtcacaatc ttgccgtaca ggttttcagc tatgaccgtg atttcatggt cagcattcac
     6361 ttctagatca tccccaaccg gcagataata aatgcctttg tggttcgtgg gggtgagctc
     6421 aacaagtttg ttatctatcg taacagcttt aatatccgcg actccggcct ggccctggaa
     6481 tgcggcaaca ataatttcat ttaagaaata cacaggagct cgtttggcca gagctgcacc
     6541 gtccttaagc accatcagtg ttgggttatc agaaagcatc agtttgcttt caggctcatt
     6601 gtacgtagag gtattcttgc tattgttctc taccgtgaac gaggcggcat aagaaccatt
     6661 cgtcaagtct gttaagtcaa agatatacag atattcatcg ccgactttct gtggcccgct
     6721 cacgggctga agagtacgac gtagcggtgt tgcccaggat tgcttaaagt cgataaccgt
     6781 accatcttct aaagtaactt ttgttgtcgg cttaaacacc ccatcaggat tcatcgttag
     6841 cgtcatagcg gttggtaacc accactcacc ctcagtttct tcctgaggca ctttgacggc
     6901 tacggccacc gttctagctc ctgtatagat ctcctgtcca ccaacactta ttgttttagc
     6961 ttcatccgtg aaattccaga gagtatggtc aacaacagga ggacggtaat cccaggtcag
     7021 cgttagcttc tgcgcttcag gaggggcaaa gtaattattt ttcgaattgg cagtgaccgt
     7081 gatttcccgt gaacccatca gcaaatccga atcacttact ttgatgcttc caacatcgat
     7141 cgtgcagcca ttcccaccaa ttgcgacttc gcaagctgat ccagacccga tagagatata
     7201 ttgcacataa ttgcgaggct ctacggagac agtgacattc ctaattgctt catccttaag
     7261 ataggattta agagtgtaac catcctgaac cactccattc atagacgatt tcaggcccgt
     7321 tatcgtcggt ttaaccggta caagagcatt aactgtatac tccccattaa ccacttctac
     7381 tttctgcctt tctgatccag aaaaataaga aattttatac ggcagtttta gttctcctgc
     7441 cgcattgggt atccctgtta aattgaatcc gtttgctgta aagccagagg tatttggaag
     7501 ccattcaaaa aagcagacca agttatctac gcttgatgtc gcacgatcat caaccgtcac
     7561 attgcatgcg ttgccagtac ctgaagcagt aattgtatta ttgttgacga atgcaacaaa
     7621 atcgcccggg ccgggattaa acgtgacggc aggcatatca gcctggagtt ttccggaaat
     7681 cataattgcc tcagcttgta ccgatttaat cgaagcaatt aaagaaaaca acagggttaa
     7741 acataaacct gattttctca tgacgaacac ctgttttttt acgcagaatt agaatcactc
     7801 tacgttaaaa aattctgaaa tcttgttcgt aaagaggaaa aaaaacatta aatttttgca
     7861 ggaaattgag gttttttagc tcactgaccg ctcttagact cagataggcg aagggcggta
     7921 attatcgatt ctgaggatac ccaaggcatt ttaagtgtca ggaaccccaa caagaacaag
     7981 cctgttgaaa cgcaatccat ttgtgttacg ggaaggttat aatttaaagg cttaaacgtc
     8041 cccattttaa ccggtacaat gcttgcgctt accaaaaggc ccccacggtc gttggggccg
     8101 atattgatat ttttgtaggc ttggtgagtg cgatgcgtgg tcttacgctt ttcaaatttt
     8161 agtgtcggct taattctcag aaaaacagat gccagcgcca tggcttcgtt ttgggatagt
     8221 ttcatgagaa tgctgccctc tgaattccat ccatattccc tattgtcaga cggaggagca
     8281 aactggagag acaggtaata cttttcagtc tcagttacaa acgccgaatt atcagcagag
     8341 cgacttttgt cgagatttac ttcgaatgcc atcgcgacag attggctgaa tactctgacc
     8401 cgttgaggct tcgcaccagt tgctgacaga tcgctcattg tcatactctc ttcttaaaga
     8461 accatgtacc ggacataact tcattatcct tcgtgtgcgt caaatccgaa ataacaaaac
     8521 caccttttcc ttccgaaatt atcatggtgt aagagctcgt atgcacaagg cgggaatttt
     8581 tgacttgcag acccgtacac ttaactgcaa tttcattacc cggaactctc tcaacaaaag
     8641 taacttttga tgcaacgcca ttgatatgta cgcttccgtt atctgctatc tggatgttca
     8701 acgaaggtgt ggattcttta tcacaaggtt ggaaaacaga agtggtttta agaactttgc
     8761 cagtaaaatc gtacaagtca ggcttcgacg gttttgcgtt aaagatcgag taagaccccg
     8821 ccactgttaa accaaccatt aaaaggggga ttaccttttt aaaattaact ttcatcctcc
     8881 agttcctctc caatcaagtg taaagcactt acgcacataa gcctgagttg atacagcaaa
     8941 ttgtcggcgg tgatcccccc aacaaatgtc actgtacggc gtatcgaaac gcctccaggc
     9001 tcgcattgaa tatggccaat acctgcatcg cgaaggaaat cattctcggc gcttaccagg
     9061 tctgcttcag caggcggaat gaccacttcg atgagtattg tatcaattgg agcccactcc
     9121 cagtcgtact taagcgatga aattggggca atggaaacat gaaattcatc aatattaaaa
     9181 atgactggtt ttttgcctgt tgatttaggc tgaatgaagg gattctcgac caggacaact
     9241 gggaattcag aaatagacag aacttcgatt aactgatctc gacgtaaacc gtgtattagt
     9301 gagttgcgtg ataacattca gtcagccttt attataataa tcagatagtt acacatgatc
     9361 ccgctagttc cttaccatct tcccgcttgc agcg
//
LOCUS       DAMZII010000173         9361 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZII010000173 DAMZII010000000
VERSION     DAMZII010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.173"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(103..2094)
                     /gene="tkt"
                     /locus_tag="POA77_004273"
     CDS             complement(103..2094)
                     /gene="tkt"
                     /locus_tag="POA77_004273"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6367614.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2372..3130
                     /gene="loiP"
                     /locus_tag="POA77_004274"
                     /gene_synonym="yggG"
     CDS             2372..3130
                     /gene="loiP"
                     /locus_tag="POA77_004274"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDI6367615.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3336..4256)
                     /gene="speB"
                     /locus_tag="POA77_004275"
     CDS             complement(3336..4256)
                     /gene="speB"
                     /locus_tag="POA77_004275"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDI6367616.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4392..5123)
                     /locus_tag="POA77_004276"
     CDS             complement(4392..5123)
                     /locus_tag="POA77_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367617.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5269..7245)
                     /gene="speA"
                     /locus_tag="POA77_004277"
     CDS             complement(5269..7245)
                     /gene="speA"
                     /locus_tag="POA77_004277"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDI6367618.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7254..7385)
                     /gene="yqgB"
                     /locus_tag="POA77_004278"
     CDS             complement(7254..7385)
                     /gene="yqgB"
                     /locus_tag="POA77_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDI6367619.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7521..7736
                     /locus_tag="POA77_004279"
     CDS             7521..7736
                     /locus_tag="POA77_004279"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367620.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8040..9194
                     /gene="metK"
                     /locus_tag="POA77_004280"
     CDS             8040..9194
                     /gene="metK"
                     /locus_tag="POA77_004280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI6367621.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2310 a         2513 c         2383 g         2155 t
ORIGIN      
        1 atttgtgatc tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa
       61 taaaaaaggt cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc
      121 tttcgcaaca acgttatcaa cagtgaagcc gaactcttca aacagcagct ctgccggagc
      181 agattcaccg aaagtggtca taccgacgat agcaccgttc aggccaacat acttgtacca
      241 gtagtcagca atacccgctt ctacagcaac gcgtgcagta accgctttcg gcagtacgga
      301 ttcacggtaa gcagcatcct gcttgtcaaa tgcgtcggta gacggcatgg acaccacgcg
      361 cgctttcacg ccttcggcag tcagtttttc gtaggcagca acagccagtt caacttctga
      421 accggtagcg atgaaaatca gttccggctg accggcgcag tctttcagca cataaccacc
      481 gcgcgcgatg tttgccagtt gctcttcagt tcgttcctgc tgcgccaggt tctgacggga
      541 gaggatcagt gcggtcgggc cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga
      601 ttcaacctgg tcacacggac gccatgtaga catgttcggg gttacgcgca gagaagcgac
      661 ctgctctacc ggctggtgag tcgggccatc ttcgcccaga ccgatggagt cgtgggtgta
      721 aaccatcacc tgacgctgtt tcatcagcgc agccatacgt acggcgttac gtgcgtattc
      781 cacgaacatc aggaaggtgg aggtgtacgg caggaaacca ccgtgcaggg agataccgtt
      841 agcaatcgcg gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc
      901 ttcgttgatt gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcaga
      961 accgccgagg aattccggca acagcggacc gaaagcttcg atagcattct gagacgcttt
     1021 acggctggcg attttcgccg ggttagcctg cagtttagca atgaactctt ttgctttcgc
     1081 gtcgaagtca gacggcattt cgcctttcat acggcgggta aattcagcgg cttcctgcgg
     1141 ataagctttc gcgtaagcag cgaatttctc gttccatgcg gattctttcg cctggcctgc
     1201 ttctttcgca tcccactgag catagatttc agacgggatt tcgaacggtg catatttcca
     1261 gcccagctgt tcgcgggtca gggcaatttc agcgtcgccc agcggcgcac cgtgggagtc
     1321 gtgagtaccg gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga
     1381 cggtttgtca gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ccgcgtcatg
     1441 accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc
     1501 ggtgaaccag ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat
     1561 cagtttaccc agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat
     1621 catgcagccg tcgcccatga aggcataggt gtagtggtcg acaatgtcgt ggcccggacg
     1681 gttaaactgc gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc
     1741 ctgacccagc ggaccggtgg tggtttccac accagcggtg taacccactt ccgggtgacc
     1801 cggagtttta gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc
     1861 ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg
     1921 gtcacggtca gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac
     1981 ttcggcaatg tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac
     2041 tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac
     2101 tccagatcgg atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta
     2161 cgccggaaaa tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt
     2221 ttgccgcttc agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg
     2281 ctgcgctttt ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag
     2341 aataatcctg accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat
     2401 gagcgtggca acggtactga ccggttgcca gaatatggac tccaacggac tgctttcatc
     2461 aggagcggaa gcttttcagg cttacagttt gagtgatgcg caggtgaaag ccctgagcga
     2521 tcaggcatgt caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc
     2581 taaacgtctg acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa
     2641 ttacaaagtg tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg
     2701 cgtctatagc gggctgatgg atatgatgac ggataacgaa gtcgaagcgg tgatcggtca
     2761 cgagatgggg cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac
     2821 aaatgccgtg cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc
     2881 acaacttggt gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc
     2941 agaagccgat gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct
     3001 tgccaccagc tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga
     3061 cgaccatcct gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg
     3121 gattaagtaa agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg
     3181 acatgaatgg cacatttgtt accttgtgcg cattgccgga tgcgatgctg gcgcatctta
     3241 tccggcctac gggtgccgaa cgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa
     3301 tggcacgttt tacccgtgcg catcgcatct ggtgcttact cgcccttttt cgccgcctga
     3361 atatacagca tctccagcgc cagcgtcgcc gcagccagag cggtgatttc cgactgatca
     3421 tatgccggag ccacttccac tacgtccatc ccaacgatgt tgagatcttt caggccgcgt
     3481 accagtttaa tagcgcgatc ggaggtcagg ccgccaatca ctggcgtacc ggtgcctggt
     3541 gcaaaagcag gatccaggca gtcgatatca aaggtcaggt aaaccggcat atcacccaca
     3601 atctgtttca cctgggcgat aacgtcatcc acgctgcgat cgttcacctg gcaggcgtcc
     3661 agcacggtaa agccgttgtc tatatcaaac tcggtacgaa taccaatctg cacggaatga
     3721 ttcgggtcga tcagaccttc tttcggcgcg gtatagaaca tggtgccgtg gtcaaattca
     3781 caaccgttcg cataggtatc ggtgtgggcg tcaaagtgta ccagcgccat tttaccgaaa
     3841 tgcttcgcat gagcacgcag cagcggcagc gtaacaaagt ggtcaccacc gaaggagagc
     3901 atacgcttac cggcagccag cagcttctcg gcgtgcgcct gcagcttctc gctcatctca
     3961 cgggcatcgc cgaaagcata taccagatca ccgcagtcca cgacgttcag acgctcacgc
     4021 atgtcgaaat tccacgggaa gcgattgtgt tcccaggcca gattcgtcga aacctgacgg
     4081 atcgctgccg gaccgtggcg accaccggca cggccagagg tggccatgtc gaacggcaca
     4141 ccagtaatca cccagtcagc atcgctgtca tacggctgga agttcatcgg caggcgtaaa
     4201 aaaccaaagg cgttggaaac cagtgagtta tcgtattgat gacctaaggt gctcatggat
     4261 ggactcctat tacaaagtcg atataaaaaa acccttccgc gtcgttaggc ccgacgagga
     4321 agggttggat ttgcgaaaca caagtttgtg ctaattatcg ccttaaatgg gcgaggcatc
     4381 aagcgaggtt gctatttccg acacggtggc ggcgcatagc cattgttatc tgctggaaaa
     4441 gcttgtccag agcacggtgg cagcgcgttt tgcgccgggc cttgtaattc aaaggcggca
     4501 aagccgatta ccccggtatt acgcacatcg ccttgcgccg aattggctgc gtaggaatca
     4561 acaacgttgg cgaactgaaa agccgcttcg gtatgtttat ctttgcggaa tcctttaatc
     4621 gctaacgaat ctccggcatt cacaatatag ccgttgttat tcagcgaacc ttgcttgccg
     4681 ttcagcacat ccaggccatc aaccgtagca acgatctcgt agttggtgtt acgactgtaa
     4741 ttacgaacat ataattgata attcattccg actctggcat ggacaatata gcgtggttca
     4801 ttattggatg aatcataaac acgcgtaata ggtagagaat taaaattacc atcgcgaata
     4861 gcatattcca gttcgttaat acgaatgcta taaacgcggt cgtagccgga aggaacatta
     4921 gtcgaataac taataacgat catctccgcc ggatcgcgcc aggctcgctc agcagatacg
     4981 ccctgaaccg ttgagtgcac atccccaccc caggcggtgc cgacattacc cgatgcactg
     5041 ctattatact gcgcattact ggaacaaccg gccagcagca cgattaatgc caccaacgcg
     5101 ctacgtactt tccatttttt cataaccgtt ccctctgcat ttaattaatg cagcgattgt
     5161 agggagatta tttaataaga aaagcagcta aattattttt aagaatatat aaaaagaaag
     5221 cggaccgatt tacgtaaata aatcgacccg ccagcagaat aatcttaatt attcatcttc
     5281 aagataggta taaccgtaca aacctgcctc gaactcttca aggaactgtt gttgcagttc
     5341 agcatcaaga tcggttttct tcacttgatc acggaactga gttaacagcg ttttcggatc
     5401 gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca gttccacttc
     5461 gacactaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga acaggttgtg
     5521 catgttgccg aggatctcct gatatgcgcc gaccataaag aaaccgagca tcggcggatt
     5581 ctctggatcg tactccggca taggcattgt cgtggcaata ccgtcaccat caatatagtg
     5641 gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcggc gttccggcac
     5701 ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc acgcatccgg
     5761 catcgactgg aacagcgaga agttgacgta cattttgtcc gccatacgtt cctgcagctc
     5821 gtcgataatc ggacgatgag cacggttttg cggatccagc tgcttttgca cttcatggca
     5881 catgctcaaa taaagctgct cagcccatgc gcgttcttgc aggctaaagg tgccggaaga
     5941 gtagccgata tgaatgtcgt gcagatccat ctgactgtcg tgtaaccatt cacgcagaga
     6001 acggcgagtt cccggttcgt gcatctcctg ccaggtttcc cacatgcttt gcagcgcacg
     6061 cggcgcatct tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca cgccgatgat
     6121 attagacacc agcacggtgt gatgcgcagt cactgcacga cccgattcag tgattaccgt
     6181 cggatgtggc agaccgtttt cttcacatgc atcaccaatc gcccagatga tgttgttggc
     6241 atattcattg aggccgtagt tcaccgaaca gtcggactgc gaacgagtac cttcataatc
     6301 cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct tgtgcagttc
     6361 cacatagaaa cgcgccgatt cacgaacgcc cgtggcgata tcgcgaatat tcgccatctg
     6421 cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt cacgcagggt
     6481 ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt ccccgccgga
     6541 ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca gacgaggaac
     6601 gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct tctcaatgac
     6661 cagatagacc ttgtggccca tcttctcgcc aattaatgcc aggcggatat attcgcggtc
     6721 tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgcgcca gcaccgccat
     6781 caactctgct ttcgagcctg cttccagccc caacggttcg ccagaatgaa tcagggactc
     6841 aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat cgccgttata
     6901 gccgtaggat tcccgcgcac gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag
     6961 gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac gagttttcac
     7021 taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca cgctgatgtg
     7081 gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt aagtacgcag
     7141 catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta gtacaccgtg
     7201 ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga acctcaaatt
     7261 attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga taacaaccca
     7321 taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac cggtttcttt
     7381 ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg aaaccacgag
     7441 aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta accagctctt
     7501 gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc agagatgcag
     7561 agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag acaacggttc
     7621 attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc aaaactctga
     7681 tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg
     7741 cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata cgcagaatgc
     7801 cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac gattcaaaaa
     7861 aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta atccatccat
     7921 accgattaac actcagactg ccagtgtttt taacttgcag agtcgtggta ggatccgcta
     7981 ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg atattaaata
     8041 tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac aaaattgctg
     8101 accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg
     8161 cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc accaccagcg
     8221 cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc tatgtgcatt
     8281 ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc aaacagtctc
     8341 ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg ggtgaccagg
     8401 gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca cctatcacct
     8461 atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc
     8521 tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa atcgttggta
     8581 tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa tcgctgcaag
     8641 aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg acttctgcca
     8701 ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg ggtgactgcg
     8761 gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt cacggtggcg
     8821 gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgctgcgtac gcagcacgtt
     8881 atgttgcaaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt caggtttcct
     8941 acgcaatcgg cgtggctgaa ccgacctcca tcatggtaga aactttcggt actgagaaag
     9001 tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc ccatacggtc
     9061 tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca tacggtcact
     9121 ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg
     9181 ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc tggccttttt
     9241 cttttacctc accgcggtgg ataggcattc acgccgtatc cggcaaaaag ctgcccgcac
     9301 aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac tgaaaccgat
     9361 t
//
LOCUS       DAMZII010000174         9335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZII010000174 DAMZII010000000
VERSION     DAMZII010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.174"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            119..724
                     /locus_tag="POA77_004281"
     CDS             119..724
                     /locus_tag="POA77_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDI6367622.1"
                     /translation="MSNKELITEFLLAAEQGNSDGLKSCLDKKVDINATNREGRTAII
                     IASLNKHYDCVSLLISAGADINKQDQTCFNPFLIGCLNNDLTLLRTVLPANPDLNCLT
                     RFGGVGITPACEKGHVEIVRELLMRTDINVNHTNFVGWTPLLEAIVLNDGGEKQQEIV
                     KLLLEHGANPHMTDKYGKTPLELAREKGYHKIAELLISAGA"
     gene            810..1658
                     /locus_tag="POA77_004282"
     CDS             810..1658
                     /locus_tag="POA77_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6367623.1"
                     /translation="MGQVEQVFSHAVNLLIPEQQRLLTLLSEDCDNAPNSCRLALTHY
                     EDLFHPGEQVNFSGSGITVGNDKWIEITGCQMWRIPRWTSDSASLAKVGWQHWEEFIR
                     QQLRSNDTLFLYQGDNPFYQEISRQLLNRRKNLLKALCTGENITAAVNQLIGLGIGLT
                     PSSDDYLVGLSTILFIQGHPLRKYRYDFLAALQLAGNNITLLSKITLEEAFNQRYRER
                     VGRLLNQFITEPQPISTQYVTDIKNIGSSSGCDMLYGMADACALSHRSGGNYVNQDSC
                     KKKHVF"
     gene            1618..3165
                     /gene="fdrA"
                     /locus_tag="POA77_004283"
     CDS             1618..3165
                     /gene="fdrA"
                     /locus_tag="POA77_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6367624.1"
                     /translation="MSIRIVVKKNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEEAKSGDLMIVIKGTSEEANERTLIAIDELFAHKEQGGQHEARYA
                     TIASAKKHAPESNLAVISVNGLFAAREARQALQNNLNVMLFSDNVSLEDELELKHLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIIGASGTGSQELSVRIHEFGGGISQ
                     LIGTGGRDMSEKIGGLMMLDAISMLEADPQTEIITLISKPPAPAVARKVLERARTCHK
                     PVVVCFLGRESTPADEEGLQFARGTKEAALKAVLLTGVKKESLDLHPLNWQLIEEVRS
                     RLTPQQKYIRGLFCGGTLCDEAMFTAMEKLSDVYSNIHPNPAFRLTDLNRSVAHTFLD
                     FGDDYFTNGKPHPMIDPTNRITRLLQEAQDPEVGVIVMDFILGFGSHEDPVGVMLDTI
                     VEAKEIAAADGRPLEILGYVLGTDLDTPSLKKQCQMLTDAGVIWASSSTNTGLLAREF
                     IFKGEEG"
     gene            3165..4583
                     /locus_tag="POA77_004284"
     CDS             3165..4583
                     /locus_tag="POA77_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6367625.1"
                     /translation="MSQTLFNQPLNVINVGIEMFSDDMKKQNVPVTQLDWTPPGQGNI
                     QVVTALDDIAASPLADKIAAANQLALERIIQSHPVLIGYDQAINVVPGMTRNTILHAG
                     PPIHWEKMCGAMKGAVTGALVFEGLAKDLDDAAELAASGEIIFSPCHEHDCVGSMAGV
                     TSASMFMHIVENKTYGNRAYTNMSEQMAKILRMGANDQSVIERLNWMRDVLGPMLRDA
                     MKLAGEIDLHLMLAQALHMGDECHNRNNAGTALLIQTLTPWIIQTGYPVEQQREVFEF
                     VLSSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRISGLPGQWFTGPA
                     QQVIGPMFAGYKLEDSGRDIGDSAITETYGVGGFAMATAPAIVSLVGGTVDEAIDFSR
                     KMREITLGENPNVTIPLLGFMGVPTAIDITRVGSTGILPVINTAIAHKDAGIGMIGAG
                     IVHPPFTCFEKAILNWRDHYCQ"
     gene            4696..6246
                     /locus_tag="POA77_004285"
     CDS             4696..6246
                     /locus_tag="POA77_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HDI6367626.1"
                     /translation="MNSIKLEWKRGDWAAYFGLMTNNLTNLLTMMGLLIFVVGIPTEI
                     VYGRIAPAFGLAVLAASVCYSWFGLQMVKKTGRSDVTALPSGPSAPSIFTVTFLVLMP
                     VYQQTKDANFAIQIGLVWCFVEALILVGGSFLGETIRKMIPRTVLLSCLSGLGLLLLA
                     MNPMLQAFEAPTVSFIVLLLIFINWFGKKPIFPHIPTGLLLLIAGTSLAWISGLQSPE
                     DIKASMSSFGFNPPEIHVRSFLQGLPHALPYLASAVPLGLANYIFDLENIESAHAAGD
                     EYNTRKVMMANGFASMLGCMLGNPFPVTVYVGHAGWKAMGASICYTLASGITMFLVPL
                     FGLGAFMLAIIPMTAIVPILVFIGVVTANQVVRETPKVEVPVIFICLFPWVANWALTI
                     VNSVMGAAGTSAEKLGSDLLHSKGVYYDGLMHLGSGAPLASMLWGCIAIFAIMNKPLR
                     GAIAASFGALLSLFGVIHSPAVGFAEGSSIVFVVAYLMMLAMFALKHVIDSRETVAAS
                     EQEETKTT"
     gene            6273..7235
                     /locus_tag="POA77_004286"
     CDS             6273..7235
                     /locus_tag="POA77_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDI6367627.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     PHGEKKAVTVVTQVEVDKNDPGFVHPTKPIGAFFSENQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKTLIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     SSALLAREIHADILVITTGVEKVSIHFGKPNQHALDTVDVLTMARYMQEGHFPPGSML
                     PKILASLEFLERGGKRVIITTPECLSAALRGETGTHIIHSQEET"
     gene            complement(8071..9335)
                     /locus_tag="POA77_004287"
                     /pseudo
     CDS             complement(8071..9335)
                     /locus_tag="POA77_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         2312 a         2232 c         2434 g         2357 t
ORIGIN      
        1 tttcaataat tcatggtcta atgatgagta aattttaaat ggatggtgct ctatgcattc
       61 tgttgctatt cttggcttgt tatatttgta aaatcttaaa aagagcagga gatcgattat
      121 gtccaataaa gaactcatta cagaatttct gctggcggca gaacaaggta attccgatgg
      181 attaaaatca tgccttgata aaaaggttga tattaatgcg actaaccgcg aggggcgtac
      241 tgcaattatt attgccagtc tgaataaaca ttatgactgc gtttctttat tgatttccgc
      301 aggtgctgat attaataaac aagatcaaac ttgttttaat cccttcctga ttggctgcct
      361 gaataatgat ttaacactgt tgcgtacggt attaccggct aaccccgacc ttaattgcct
      421 aacccgtttt ggcggtgttg gtattacacc tgcctgcgaa aaaggccatg tcgagattgt
      481 acgtgaattg ctgatgcgta ctgatatcaa tgtcaaccat actaattttg ttggctggac
      541 gccgctccta gaggccatcg tgctgaatga cggcggtgaa aagcagcagg agatcgtcaa
      601 actgttgctt gagcatggcg caaacccgca catgacggat aaatacggta aaaccccgct
      661 ggagctggcg cgagaaaaag gctaccacaa aattgccgag ttgctgattt cggctggagc
      721 ctgacaacag gagcacgatt atcgctatat tatcacggca atcttttcgg gcactaaccg
      781 cagactgcgc gtttctcact tttcggggaa tgggccaggt tgaacaggtt ttttcccatg
      841 cggtaaatct gttaatacct gaacagcagc gactgctgac gttactgagt gaggattgtg
      901 ataacgcacc taacagttgt cgtttagcac tgacacatta tgaagacctt tttcaccccg
      961 gtgaacaggt caattttagc gggtctggaa taactgttgg aaatgataaa tggatagaaa
     1021 taaccggttg tcagatgtgg cgaataccac ggtggacatc tgattcagca agccttgcca
     1081 aagtcggctg gcagcattgg gaagagttta tccgccaaca actaagaagc aacgacacat
     1141 tatttctgta ccagggtgac aatccatttt atcaggaaat ttctcgccaa ttactcaacc
     1201 gccgaaaaaa tttgctgaag gcgttgtgta caggagaaaa catcactgcg gcagtcaacc
     1261 agttaattgg tcttggaata gggctgacgc catcatcgga tgattattta gtaggtttaa
     1321 gcactatttt atttattcaa ggacatccac taaggaagta tagatacgat ttcttagcag
     1381 cattacaact tgcaggaaat aacataacgc ttctcagcaa aataacgtta gaggaagcat
     1441 ttaatcagcg ttatcgggaa agagtcggac ggctactgaa ccaatttatt actgagccac
     1501 aacccatttc tacgcaatat gttactgaca taaaaaatat aggctcaagt tctggatgcg
     1561 acatgctgta tggcatggcg gatgcctgcg ccctgagcca caggtccgga ggtaattatg
     1621 tcaatcagga tagttgtaaa aaaaaacacg tattttgatt ctgtttcact gatgtctatc
     1681 tccacgcgcg caaataagct tgatggagtt gagcaggcat ttgttgctat ggcgacagag
     1741 atgaataagg gggtgctgaa gaatctcggt ctgctgaccc ctgagctgga agaggcgaaa
     1801 agtggcgatc tgatgattgt cattaaaggt accagcgagg aggcgaacga gcggacgcta
     1861 atagcgattg atgagctgtt tgcgcataaa gaacagggag gccagcacga agcacgctat
     1921 gccacgattg ccagtgcgaa gaagcatgct ccagagagta atctggcggt gatctctgtc
     1981 aatggcctgt ttgctgctcg tgaagctcgt caggcactac aaaataacct caatgttatg
     2041 ctgttttctg acaacgtctc tcttgaggac gagctggaac tcaagcatct ggcacatgaa
     2101 aaagggttgt tgatgatggg gcctgattgt ggaaccgcca tcattaacgg cgcagcgttg
     2161 tgttttggca atgcagtgcg tcgcggcaac atcggaatca ttggtgcctc cggtacgggt
     2221 agccaggagc tgagcgtacg cattcacgaa tttggcggcg ggatttccca gctgattggg
     2281 acaggtggac gcgatatgag tgaaaaaatt ggcggcctga tgatgcttga cgcgattagc
     2341 atgctggagg ccgatccaca aactgagatc attacgctta tatccaaacc tcctgcacca
     2401 gcggtagccc gtaaggtgct ggaacgtgcg cgcacctgtc acaagcctgt ggtggtgtgt
     2461 ttcctcggac gtgagtccac ccctgcggat gaagaaggcc tacagtttgc ccgcggtact
     2521 aaagaggctg ccttgaaagc ggtactgttg actggtgtga aaaaagagag tctggatttg
     2581 catccgctca actggcagct gattgaagag gttcgctccc gtctgactcc acaacaaaag
     2641 tatatccgtg gcctgttctg tggtggcacg ttatgcgatg aagcgatgtt tactgccatg
     2701 gaaaaactct cggatgtgta tagcaatatt cacccgaatc cggcgttccg ccttacagat
     2761 ctcaaccgta gcgtggcgca taccttcctc gatttcggcg atgattattt taccaacggc
     2821 aaaccacatc cgatgatcga tcccaccaat cgcattaccc ggctcttgca ggaagcacag
     2881 gatcctgaag ttggcgtgat cgtgatggac ttcattcttg gatttggatc acatgaagat
     2941 ccggttggag tgatgctgga tacaattgtt gaagccaaag aaattgctgc cgcagacggt
     3001 cgcccgcttg agatcctcgg ctatgtgctg ggaaccgacc ttgatacacc gtcgctgaaa
     3061 aaacagtgtc agatgttgac cgatgccggg gttatttggg caagcagtag caccaacacc
     3121 ggattactgg cacgtgaatt tatatttaaa ggggaggaag gctgatgagc cagacactgt
     3181 ttaatcagcc gctgaacgtt attaatgtcg gtatcgaaat gttcagcgat gacatgaaaa
     3241 agcagaatgt accggtaacc caacttgact ggacaccgcc aggccaagga aacatacaag
     3301 tggtaacggc actggacgac attgccgctt caccactggc agacaaaatt gcagctgcta
     3361 atcagctggc gctggaacgc attatccagt cgcatcctgt gctaattggc tatgatcagg
     3421 cgatcaacgt cgtgccgggt atgacccgta acactatcct tcacgccggg ccgccaatcc
     3481 actgggaaaa aatgtgcggc gcaatgaagg gcgcagtaac cggggctctg gtatttgaag
     3541 gactggcgaa agatctggat gatgccgccg aactagctgc ttcaggtgaa atcatcttct
     3601 ctccgtgcca cgaacatgac tgtgtcggct caatggcggg tgtcacgtct gcatcgatgt
     3661 ttatgcacat cgtcgagaac aaaacctatg gaaaccgcgc atataccaat atgagcgagc
     3721 agatggcaaa gatcctgcgt atgggcgcta acgaccagag cgttatagag cgtcttaact
     3781 ggatgcgaga tgtactggga cctatgttgc gcgatgctat gaagctggcg ggcgaaatcg
     3841 atctgcactt gatgttagcg caggcgctgc atatgggcga tgagtgccat aaccgcaaca
     3901 acgccgggac ggcgctgctg attcagacgc tgacgccgtg gattattcag actggctacc
     3961 ctgttgagca gcagcgtgag gtgttcgagt ttgtcctcag tagtgattac ttctcaggac
     4021 caacatggat ggcaatgtgt aaagccgcaa tggacgctgc gcacggcatt gaatacagca
     4081 ccgtggtgac aacgatggca cgcaatggtg ttgagttcgg tcttcgtatt agcggtttgc
     4141 cgggccagtg gtttaccggt ccggcgcagc aggtgattgg gccgatgttt gcgggctata
     4201 agctagagga ttccggacgt gatatcggtg atagtgcaat taccgaaacc tacggcgttg
     4261 gcggtttcgc tatggcgacg gcaccagcta ttgtgtcact ggtgggcgga acagttgatg
     4321 aagctattga tttctcacgt aaaatgagag aaatcactct cggagaaaac cccaacgtca
     4381 ccattcccct gcttggtttt atgggcgtac cgaccgctat cgatattacg cgtgtaggca
     4441 gtacaggcat tctgccggta attaacacag ccattgcgca caaagacgca gggatcggca
     4501 tgatcggcgc aggaattgtt cacccgccgt tcacttgttt tgaaaaggcg atcctcaact
     4561 ggcgtgacca ctactgccaa taacaataac cggggctaac gcagttagcc ccacagtagc
     4621 ggaaaacgtt gtttaccttc agggctgggc gatctccatg atcgcaaacg atgcttaacc
     4681 tgttaaggag attccatgaa cagcataaaa ctagaatgga aacgtggtga ctgggcggct
     4741 tactttggac tgatgaccaa caatctgact aacctgctga caatgatggg tctgctaatt
     4801 tttgtcgtcg gaatccctac tgaaattgtc tatggacgca ttgcgcctgc ctttggtctt
     4861 gcagtgctgg cggccagtgt ctgctactcc tggtttggtc tacagatggt gaagaaaacc
     4921 ggtcgtagcg atgtaacggc gctgccgtct ggaccgagcg caccctcaat ctttaccgtg
     4981 acattcctgg tgctgatgcc ggtttatcag caaaccaaag atgccaactt tgctattcag
     5041 ataggattgg tgtggtgctt tgtcgaagcg ttgatcctgg tcggtggatc cttcctgggt
     5101 gagactatcc gcaagatgat cccgcgtaca gtgctgcttt cttgtctgtc cggactgggg
     5161 ttattactgc tggcaatgaa cccgatgctg caagcgttcg aagcgccgac agtttctttc
     5221 atcgtcctgt tattgatctt cattaactgg ttcggtaaga agccgatttt tccccatatt
     5281 cctaccggtt tgctgttgct gattgctggt acgtccctag cgtggatatc cggtttacaa
     5341 agcccggaag acattaaagc gtcgatgtct tccttcggct tcaacccgcc ggaaattcat
     5401 gtaaggagct tcctgcaagg tttgccgcac gctctgccat atctggcttc tgcagttcct
     5461 ttgggcctgg ctaactacat ttttgacctc gagaacattg aaagtgcgca cgcggctggt
     5521 gatgaataca acacccgtaa ggtcatgatg gcgaacggct ttgcctccat gctcggttgc
     5581 atgctgggta atccgttccc ggtaaccgta tatgttgggc atgcaggctg gaaagctatg
     5641 ggcgctagta tctgctatac cctcgcatca ggcatcacta tgtttctggt gccattgttt
     5701 gggttgggcg cgtttatgct cgccattatt cccatgactg ctatagtgcc aattctggtt
     5761 tttatcggtg tggtaaccgc caatcaggtg gtgcgagaaa ctcctaaagt cgaggttccc
     5821 gtcattttca tctgtctgtt cccatgggtc gccaactggg cattgaccat cgttaacagc
     5881 gtgatgggcg cagccggaac atccgctgag aaacttggta gcgacctgtt gcacagcaaa
     5941 ggcgtctatt acgatggact gatgcatctg ggaagcggtg caccgctggc cagcatgctg
     6001 tggggatgca ttgctatctt tgccattatg aataagccgc tgcgcggggc gatagctgca
     6061 tcctttggag cactgttatc gctgtttggc gtgattcact ccccagcggt tggctttgcg
     6121 gaaggcagct cgattgtgtt tgttgtggcc tacctgatga tgcttgctat gtttgccctc
     6181 aagcatgtta ttgatagccg tgaaacagtg gctgcgtctg agcaggaaga gactaaaacg
     6241 acctgaatag cctgcaaacg gaagataatt ctatgaaaga acttgtggtc gttgccattg
     6301 gtggcaacag cattatcaaa gataacgcca gccagtcgat tgagcatcag gcggaggcgg
     6361 tgaaggccgt cgccgatacg gttctggaaa tgctggcttc cgattacgac attgtgctga
     6421 cccacggcaa cgggccgcag gtcggtctgg atttacgccg cgcggagata gcccacgagc
     6481 gcgaagggct ccccttaacg ccgctggcga actgtgtggc ggatacgcaa ggcggcattg
     6541 gctatctgat ccagcaggcg ctgaacaacc ggctggcgcc tcacggcgag aaaaaagccg
     6601 tcaccgtggt gactcaggtg gaagtggata aaaacgatcc ggggtttgtc catcccacca
     6661 agcccatcgg tgcattcttt agtgaaaacc agcgtgatga actacaaaag gcaaaccctg
     6721 actggcgttt tgttgaagat gccggacggg gctatcgccg cgtggtcgcc tcgccggaac
     6781 cgaaacgtat tgtcgaagca cctgccatta agactctgat ccagcaaggc tttgtggtga
     6841 ttggcgcggg cggcggtggc attccggtgg tgcgtactga cgcaggggat taccaaagcg
     6901 tggacgcggt tatcgacaaa gatctctcta gcgcgctact ggctcgcgaa attcacgccg
     6961 acattctggt gatcaccacc ggcgtggaaa aagtgagtat tcattttggg aaacccaacc
     7021 agcatgcact ggatacagtg gatgtcctaa cgatggcccg ttacatgcag gagggtcact
     7081 tcccgcctgg gagtatgtta ccgaagattc ttgccagtct ggagttcctg gaacgtggag
     7141 gtaaaagagt gattatcacc acgcctgaat gcctgtccgc ggcactgcgt ggcgaaacag
     7201 gcacccatat tatccactcc caagaggaga cgtaaattaa ggaaagcgac cgccgccgtg
     7261 aatttttaat gttcattgca gattatcgtg ggctggaaat gataacggtt ggggagttat
     7321 tgtttagttg ctaaaacaga acgataacca accccaaccg ttctcaattt cgaatactat
     7381 cttttccatt tatctctgga taggctttga cgtcgtccct actgagtaaa atccccctca
     7441 tcatccacct aaaaccgatt gaaaacggtc attactcttg tggcaaacag gctaaagccg
     7501 ttcacgatgc ttcacttcgc accttatcgt aatgaacaat actggtattt ccagttcagt
     7561 tacgcctacc cgtctaccgc attctttgcc ctaactacgg catagttcgg gattatatca
     7621 gctggctaaa gccttatgct catcagacca catgactcat tgagcatgtt gaggcgttac
     7681 tcaaaatctt gccgattaaa catatttcgt aatttcttac cctgcactag catatcatca
     7741 atactatcga caaacgtcag ttgcaaatag aggtcacagg gtctgattga tgtactgcca
     7801 aggccgggtc atgaaagagt ttgccctgtt caaagctcat cgttacgcac cgtcgtagcc
     7861 gatgcggata ctcatcaatc ctctggatag gtgaggtgcg tagtagagaa gctcggtcgt
     7921 ttttcgttaa tctgaatcag tactgtcaac atattcaagt cgttaatatg gatatgaata
     7981 cctcctttga cctagaggta cagatgcact accccccaag ctgtcaacga cggatgaaaa
     8041 gtggctcttc cgcaacctgg atggagattt tcatgccagt ggactcatga ccctctgcct
     8101 gagtagttca aatccggctc gaccatacat ctggcctttc agcattttta gccgatttac
     8161 atgactttcg acacccccca ttattccagc gactgctgat tgcctcacat atagccgcag
     8221 catcagctgc catccttgca gccacgcgcc gaagttacca ctttctcgtt gctcttttaa
     8281 ccactccctc catgggtcca gaagtcctcg ctctggaggc cgggttgaca tttcagggaa
     8341 tgttcctgaa cacacccaga gactgactgt cacacgtgat aagcctgtaa tacgggataa
     8401 gcctgtaata cgggatattt cccctgaacc cacatccctt gttatgtagt gccataacct
     8461 ctgtccaacg ctgatgccgt ttttggcggg tttgctgttt aaggcgttcc ggacgacgga
     8521 gcgatgctac aggcacagac agtactggct taggtgattt ctttggtgac aacccactgg
     8581 caacaagacg tatcagaggc atctgtctgt acatcattcg ttcaggcgca tcgccaatat
     8641 tttttagcag gtgccatcga tcagctatct gtctggcctg aggtgcacct tcacgtgctg
     8701 ctgtcgcata gactccgcca cggtcacgtg agacaacctg tatttccgga tattttctga
     8761 accaggtcgc cagcgtactc tgatcgcaac cgggaagcag gacgagggga cggtgagtat
     8821 caagattgac gattaacgtg ccgtaatggt ggccccgatg ccacgcccac tcatcaatac
     8881 cgacatgagg cgcgcccgac tgtttcgtct ccggggtatt aataaccctg cgaagaagag
     8941 tatcagcact gcaacggagc cccactgccg ttgctgcctg tttcccggcc tcacctccgg
     9001 ctattaatcc cagttgatgt tgtaaatttt gtaacacctg tgaagaatgc tgggagtgaa
     9061 ccggcgaagg gggcaagcga cccggcaaaa attttacgtg aacaagccgg gttacggcag
     9121 taccagtggc ggacggaaaa tacaagccat agcgtctgcc ctgagcaggg tagatgctgt
     9181 atccggcgcc gacgataact atgaacagag tcactacgct taaagcattc aggacttgtg
     9241 ctgttttgcg ttttccgtgg agatggaggg ttataccgtc agagctaata atttgtcgac
     9301 tgcatctcca tccgcaaggt aactgtagaa gtgtc
//
LOCUS       DAMZII010000175         9027 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZII010000175 DAMZII010000000
VERSION     DAMZII010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.175"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..995
                     /locus_tag="POA77_004288"
     CDS             <1..995
                     /locus_tag="POA77_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6367628.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVL
                     EPEGQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTS
                     AQKATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            998..1858
                     /locus_tag="POA77_004289"
     CDS             998..1858
                     /locus_tag="POA77_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDI6367629.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            1960..3483
                     /gene="istA"
                     /locus_tag="POA77_004290"
     CDS             1960..3483
                     /gene="istA"
                     /locus_tag="POA77_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HDI6367630.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            3473..4255
                     /gene="istB"
                     /locus_tag="POA77_004291"
     CDS             3473..4255
                     /gene="istB"
                     /locus_tag="POA77_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HDI6367631.1"
                     /translation="MQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSLI
                     KAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNVV
                     LIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLLY
                     ADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTTA
                     LLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(4431..4931)
                     /locus_tag="POA77_004292"
     CDS             complement(4431..4931)
                     /locus_tag="POA77_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6367632.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(5059..5898)
                     /gene="sul1"
                     /locus_tag="POA77_004293"
     CDS             complement(5059..5898)
                     /gene="sul1"
                     /locus_tag="POA77_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDI6367633.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(5892..6239)
                     /locus_tag="POA77_004294"
     CDS             complement(5892..6239)
                     /locus_tag="POA77_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDI6367634.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(6457..7692)
                     /locus_tag="POA77_004295"
     CDS             complement(6457..7692)
                     /locus_tag="POA77_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HDI6367635.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
     gene            complement(7960..>9027)
                     /locus_tag="POA77_004296"
     CDS             complement(7960..>9027)
                     /locus_tag="POA77_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6367636.1"
                     /translation="GIVHRVIAGWLADQAGVDHASAQCGAVTLIQRFGSALNLNVHFH
                     MLWLDGVYEDTTERPQRKPRLHRTRAPTSAQLTELAGTIAHRVCRHLSRRGWLEGEDE
                     SVFLSDSAGSDDGMDGLRMSSMTYRIATGRDAGRKVVTLQTLPGDAGSLEGDAGKVGG
                     FSLHAGVAAEAHESHKLEKLCRYITRPAISEQRLSISPQGRVRYQLKTPWRNGTTHVE
                     WDAVDFIAKLAALVPPPRAHLTRFHGVFAPNANLRAQLTPSGRGRRPAGDAVPVDASA
                     HDEPRSPEQKRRAMSWAQRLKRVFSIDVTTCAHCGGAVRIVASIEDPKAIRAILAHFE
                     KHGALEQAHYRPAARAPPPAA"
BASE COUNT         1798 a         2756 c         2735 g         1738 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccgatgcgc tcaagccgtg gattgcgcgg cgtgaacgct
      601 ggccgtcctt tctgatccgg cgcgatccgc gcgacatcag ccgtatctgg gtcctggaac
      661 cggagggaca gcattacctg gaaattccct accgtacctt gtcgcatccg gctgtcaccc
      721 tctgggaaca acggcaggcg ctggcgaaac tgcggcagca agggcgcgaa caggtggatg
      781 agtcggcgct gttccgcatg atcggccaga tgcgtgagat tgtgaccagc gcgcagaagg
      841 ccacacgcaa ggcgcggcgt gacgcggatc gccgccagca cctcaagaca tcagctcggc
      901 cggacaagcc cgttccgccg gatacggata ttgccgaccc gcaggcagac aacttgccac
      961 ccgccaaacc gttcgaccag attgaggagt ggtagccgtg gacgaatatc ccatcatcga
     1021 cctgtcccac ctgctgccgg cggcccaggg cttggcccgt cttccggcgg acgagcgcat
     1081 ccagcgcctt cgcgccgacc gctggatcgg ctatccgcgc gcagtcgagg cgctgaaccg
     1141 gctggaagcc ctttatgcgt ggccaaacaa gcaacgcatg cccaacctgc tgctggttgg
     1201 cccgaccaac aatggcaagt cgatgatcgt cgagaagttc cgccgcaccc acccggccag
     1261 ctccgacgcc gaccaggagc acatcccggt gttggtcgtg cagatgccgt ccgagccgtc
     1321 cgtgatccgc ttctacgtcg cgctgctcgc cgcgatgggc gcgccgctgc gcccacgccc
     1381 acggttgccg gaaatggagc aactggctct ggcactgctg cgcaaggtcg gcgtgcgcat
     1441 gctggtgatc gacgagctgc acaacgtgct ggccggcaac agcgtcaacc gccgggaatt
     1501 cctcaacctg ctgcgcttcc tcggcaacga actgcgcatc ccgttggttg gggtaggcac
     1561 gcgcgacgcc tacctagcca tccgctccga tgaccagttg gaaaatcgct tcgagccgat
     1621 gatgctgccg gtatgggagg ccaacgacga ttgctgctca ctgctggcca gcttcgccgc
     1681 ttcgctcccg ctgcgccggc cttccccaat tgccacgctg gacatggctc gctacctgct
     1741 cacacgcagc gagggcacca taggggaact ggcgcacttg ctgatggcgg cggccatcgt
     1801 cgccgtggag agcggcgagg aagcgatcaa ccatcgcaca ctcagcatgg cctgttgagt
     1861 tgcatctaaa attgacccac ttagggtaaa gatttgcgtc gaaatttgac ccacgtatga
     1921 cactgtttcc cgtctggata tggcgggaga aatcaaggag tgataaacgt ggcgatattg
     1981 agcgcaattc gacgctggca ttttcgcgat ggtgcgtcga ttcgggaaat agcccgacga
     2041 agcggcctgt ccaggaacac cgttcgcaag tatttgcaaa gcaaggtggt tgaaccgcag
     2101 tacccagcgc gagacagcgt tggcaagtta agtccttttg agcccaagtt aaggcagtgg
     2161 ctctccaccg agcacaaaaa gacaaagaag ctgcgcagaa acctgcgcag catgtaccgg
     2221 gatttggtcg ctttgggctt taccgggtct tatgaccgag tgtgtgcctt tgcccgacag
     2281 tggaaagatt ccgaacagtt caaggcgcaa acctcgggca agggttgttt catccccttg
     2341 cgctttgctt gtggcgaagc cttccaattc gattggagtg aggactttgc ccgcatagcg
     2401 ggcaaacagg tcaaacttca gattgcccag tttaagttgg cccacagccg ggcctttgtg
     2461 cttcgggctt actaccagca aaaacatgaa atgctgtttg atgcccactg gcatgccttt
     2521 caaatcttcg gtggcattcc caagcgcggc atctacgaca acatgaagac cgctgtggat
     2581 tcggtggggc gtggcaaaga gcgcagggtc aatcagcggt tcactgccat ggtcagccac
     2641 tacctgtttg atgcgcagtt ctgtaatcca gcatcgggtt gggagaaagg ccagattgag
     2701 aagaacgtgc aggattcccg ccaacgcctg tggcaagggg caccagactt tcaaagcctt
     2761 gctgatttga atgtgtggct tgagcatcgc tgcaaagcgc tgtggtctga gctgcgccac
     2821 cccgaattgg accaaaccgt gcaagaggcc tttgccgatg aacaaggcga gttgatggcg
     2881 ctacccaatg cctttgatgc attcgtggag caaaccaagc gagtcacttc aacctgcctt
     2941 gttcaccacg agggcaatcg ctacagcgtt cctgccagtt acgccaacag ggccatcagc
     3001 cttcggattt atgcagacaa gctggtgatg gctgccgaag gccaacacat tgccgagcat
     3061 ccaagattgt ttggcagtgg ccacgctcgg cgtggccaca cacaatacga ctggcaccat
     3121 tacttgtctg tgcttcagaa gaaacctggg gcgttgcgca atggtgcgcc atttgctgaa
     3181 ttgccacccg cgttcaagaa gcttcaatcc atcttgctgc aacgccccgg cggtgaccgt
     3241 gacatggtgg aaattctggc ccttgtattg caccacgatg aaggtgcggt actcagtgct
     3301 gtggaattgg cattggagtg tggcaagcca tcgaaggagc atgtgcttaa tctgttggga
     3361 cgtttgaccg aagaacctcc acccaaaccg attccaattc ccaaggggtt aaggctgaca
     3421 ttggaaccac aggccaacgt gaaccgctat gacagtttaa ggagagccca tgatgcagca
     3481 tgaaggccat gtgagaatcc tcaaatcctt gaaactcttt ggcatggcac acgccattga
     3541 ggagttgggc aatcagaatt caccagcatt taatcaagcc ttgcccatgc tggacagctt
     3601 gattaaagct gaagtggcag agcgtgaagt acgttcggtg aactatcaat tgcgggtggc
     3661 caagttcccc gtgtatcggg acttggtggg ctttgacttc agtcaaagcc tggttaatga
     3721 ggccacggtc aaacaattgc accggtgcga cttcatggaa caagcccaga acgtggtgct
     3781 gattggtggg ccaggcacag gcaagactca cctggccaca gccattggta cacaagcagt
     3841 gatgcacttg aaccgacggg tgcgtttctt ctccaccgtg gatttggtca atgcactgga
     3901 gcaagagaaa tcatctgggc gtcagggaca aatcgcaaac cgtctgttgt atgccgattt
     3961 ggtgattctg gatgagctgg gatatttgcc ttttagccaa accggtgggg cactgctgtt
     4021 tcacctgctc tcaaagctgt acgaaaaaac cagcgtgata ctgaccacca acttgagctt
     4081 ctcggaatgg agccgagtgt ttggcgatga aaagatgaca acagcgttgt tggaccgact
     4141 aacccaccac tgccacatcc tggaaaccgg caatgaaagt taccgcttca aacacagttc
     4201 aactcagaat aagcaggagg aaaaacagac ccgcaaactg aaaatcgaga cataattctg
     4261 acaacaaggg gtgggtcaaa attcaatgca aatcccgggt caaatttggg tgcaaatcaa
     4321 cagatatcga caacctctcg cgcaaccaag acatcgcggt cggactgcaa gtgatcttga
     4381 agccacgggc ccgtcccacc ccgacatgga cctcgatgcc cgaacggacg ttagatttcg
     4441 agttctaggc gttctgcgat gaaggttgga tcccagccgg gattgaaagt gtcgacgtgg
     4501 gtgaatccga gccgctcgta taggccacgc aggttcgggt ggcagtcgag ccgcagcttg
     4561 gcgcacccct gcgttcgcgc ggcatggcgg caagcctcga tcagcgcgga gctgacaccc
     4621 cggcccgcat gtgtccgtcg caccgcgagc ttgtgcagat atgcggcctc ccccttgagg
     4681 gcgtcgggcc agaactcggg atcctcggcc gacaaggtgc aacagccgac gatgccgtcg
     4741 ctgcaactcg cgactaggag ctcggatctc aggacgaagg tctccgcgaa tgtccggtcg
     4801 atccgcgcga cgtcccaggc gggcgttccc ttggcggaca tccacgccgc agcgtcgtgc
     4861 atcagccgca caacctcgtc gatatcaccc gagcaggcga cccgaacgtt cggaggctcc
     4921 tcgctgtcca ttcgctcccc tggcgcggta tgaaccgccg cctcatagtg cagtttgatc
     4981 ctgacgagcc cagcatgtct gcgcccacct tcgcggaacc tgaccagggt ccgctagcgg
     5041 gcggccggaa ggtgaatgct aggcatgatc taaccctcgg tctctggcgt cgcgactgcg
     5101 aaatttcgcg agggtttccg agaaggtgat tgcgcttcgc agatctccag gcgcgtgggt
     5161 gcggacgtag tcagcgccat tgccgatcgc gtgaagttcc gccgcaaggc tcgctggacc
     5221 cagatccttt acaggaaggc caacggtggc gcccaagaag gatttccgcg acaccgagac
     5281 caatagcgga agccccaacg ccgacttcag cttttgaagg ttcgacagca cgtgcagcga
     5341 tgtttccggt gcggggctca agaaaaatcc catccccgga tcgaggatga gccggtcggc
     5401 agcgaccccg ctccgtcgca aggcggaaac ccgcgcctcg aagaaccgca caatctcgtc
     5461 gagcgcgtct tcgggtcgaa ggtgaccggt gcgggtggcg atgccatccc gctgcgctga
     5521 gtgcataacc accagcctgc agtccgcctc agcaatatcg ggatagagcg cagggtcagg
     5581 aaatccttgg atatcgttca ggtagcccac gccgcgcttg agcgcatagc gctgggtttc
     5641 cggttggaag ctgtcgattg aaacacggtg catctgatcg gacagggcgt ctaagagcgg
     5701 cgcaatacgt ctgatctcat cggccggcga tacaggcctc gcgtccggat ggctggcggc
     5761 cggtccgaca tccacgacgt ctgatccgac tcgcagcatt tcgatcgccg cggtgacagc
     5821 gccggcgggg tctagccgcc ggctctcatc gaagaaggag tcctcggtga gattcagaat
     5881 gccgaacacc gtcaccatgg cgtcggcctc cgcagcgact tccacgatgg ggatcgggcg
     5941 agcaaaaagg cagcaattat gagccccata cctacaaagc cccacgcatc aagcttttgc
     6001 ccatgaagca accaggcaat ggctgtaatt atgacgacgc cgagtcccga ccagactgca
     6061 taagcaacac cgacagggat ggatttcaga accagagaaa gaaaataaaa tgcgatgcca
     6121 taaccgatta tgacaacggc ggaaggggca agcttagtaa agccctcgct agattttaat
     6181 gcggatgttg cgattacttc gccaactatt gcgataacaa gaaaaagcca gcctttcatg
     6241 atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact
     6301 tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaactt tgtgggtata
     6361 ggaagttcaa ccgccctttt ttgcgagtct ggcaggtagg aagcgggggc gtgtccgatt
     6421 ttttgtgtgt gaggcggttt agtccggaag tctctttcag cgagccagcg tgaatcggtc
     6481 ttcgtagatt atcgcgaact ggttcatcgc cgccttccag tgctgagacg cgcggctcca
     6541 ctcggcggtg atgttccgca gcgccagcca gatcagcttg gtggcggcct cgtcgctggg
     6601 gaagtggccg cgggtcttga tgatcttgcg caggcgcgca tgcacgctct cgattgcgtt
     6661 ggtcgtgtag atcacccggc gcacgtccgg cgggaacgcg aagaacggca ccacgcgcgt
     6721 ccaggcacgg cgccaggcgg cgaccaccgt cgggaagcgc tcgccccagt cgccggcctc
     6781 gaacgcatcc agctcggcct ctgcggcctc ggcggtcggg gccgtgtaga tcggcttgat
     6841 cgccgccgcc agcgccttgc ggtccttcca gcccgcatag tccaggctgt tgcggatcag
     6901 gtgcacgatg caggtctgca gcgtggtcgc cgggaacacc gcttccagcg cctccgggaa
     6961 gcccttcagg ccgtcagcca cgccgatcag gatgtcgttg acgccgcgcg tcttgaggtc
     7021 gttgaacacc ttcatccaga acttcgcgcc ctcggtgttc tcgatccaca gcccgaggat
     7081 gtcgcgggtg ccgtcggcca gcacgcccag cgccagatac accgccttgt tgcgcaccac
     7141 gccgtcctcg cggatcttca cccgcagcgc gtcgaagaac accaccggat acatcgcttc
     7201 cagcggccgc gcctgccatg cggtgacctc ggccatcacc tcgtcggtga ccttgctgat
     7261 gaactccggc gacacctcga cgctgtacat ctcggccagg tgcccctgga tctcacgcac
     7321 cgtcatgccg cgggcgtaca tcgagacgat ccgatcgtcg aacccggcga agcgacgctc
     7381 gtgcttgggg atcagctgcg gctcgaacgt acccgtccgg tcgcgcggca cgtcgatgcg
     7441 cagcggaccc tcgtcggtca gcaccgtctt gccgctgcgg ccgttgcggt ggttgccgct
     7501 cccgccctcg gcgtcggcca ggtgcaggcc gagctccgcg cccagcgccc gctcgatcac
     7561 ggccttcttc agctgctgga acacggcttc gaccccggcc gcgtccatcg gacccgggat
     7621 cagcttctcg agaaattcct tcggcagatc aatggccggc gtgaccaccg gcgtcttctt
     7681 cttgcgtggc atacatgctc cttggcagca tcatggtatg cctctcacac aaaatttctg
     7741 acaccctcgg aagcggccgc gcaagcgccg ctttgggcac agatcgggca cagatcgggg
     7801 gttcatgcgt gggttttcga catcgcgggg ccacgcagcg gctcagtgcg tagccatttc
     7861 tcaccgaaac ccggcacagg gccgagcgca gccctgcggg gtcgtggccg catcggatcg
     7921 gactctggct tggctgccgg ctgtgtggcc ggcacctcgt cacgcggccg gcggcggcgc
     7981 gcgcgctgcg ggccggtagt gcgcttgctc cagcgcgccg tgtttctcga agtgggcgag
     8041 gatggcgcga atggccttgg ggtcttcgat gctggcgacg atccgcaccg cgccgccgca
     8101 gtgggcacag gtggtgacgt cgatggaaaa gacccgcttg agccgttgcg cccagctcat
     8161 cgcacggcgc ttctgctcgg ggctgcgcgg ctcgtcgtgg gcgcttgcgt caaccggcac
     8221 cgcatcgccc gcaggccgcc tgccgcgccc cgagggcgtc agctgcgcac gcaggtttgc
     8281 attcggggcg aatacgccgt ggaagcgggt gagatgcgcg cgaggtggcg ggaccagtgc
     8341 cgccagcttg gcgatgaagt ccaccgcatc ccattcgaca tgcgtggtcc catttcgcca
     8401 cggcgtcttg agctgatacc gtaccctacc ctgcggtgag atcgacagcc gctgctcgct
     8461 gatcgccggg cgcgtgatgt agcggcacag cttttcgagc ttgtggcttt cgtgtgcttc
     8521 cgcggccacg ccggcatgca gcgagaagcc gccgaccttg ccggcgtcgc cctccagcga
     8581 gcctgcgtca ccgggcaatg tttgcagcgt gacgaccttg cggccagcgt cgcgaccggt
     8641 ggcgatgcgg taggtcatcg aactcatccg cagcccatcc atgccgtcgt cgctacccgc
     8701 gctgtcggac aggaacacgg attcgtcttc gccttcgagc cagccgcggc gcgacaggtg
     8761 ccggcacacg cgatgcgcga tggtgccggc cagttccgtc agttgcgccg atgtgggcgc
     8821 acgggtgcgg tgcaggcgcg gcttgcgctg cggacgctcg gtggtgtcct cgtacacgcc
     8881 gtcgagccac agcatgtgga agtgaacatt caggttcagc gcgctgccga agcgctggat
     8941 cagggtcacc gcgccgcatt gcgcgctggc gtggtcgacg ccggcttgat cggccaacca
     9001 gccggcgatc acacgatgca cgatgcc
//
LOCUS       DAMZII010000176         8961 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZII010000176 DAMZII010000000
VERSION     DAMZII010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.176"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            51..323
                     /locus_tag="POA77_004297"
     CDS             51..323
                     /locus_tag="POA77_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDI6367637.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(324..1196)
                     /gene="rluF"
                     /locus_tag="POA77_004298"
     CDS             complement(324..1196)
                     /gene="rluF"
                     /locus_tag="POA77_004298"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDI6367638.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1408..2097
                     /gene="pepE"
                     /locus_tag="POA77_004299"
     CDS             1408..2097
                     /gene="pepE"
                     /locus_tag="POA77_004299"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDI6367639.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2188..3819)
                     /locus_tag="POA77_004300"
     CDS             complement(2188..3819)
                     /locus_tag="POA77_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI6367640.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4039..7722)
                     /gene="metH"
                     /locus_tag="POA77_004301"
     CDS             complement(4039..7722)
                     /gene="metH"
                     /locus_tag="POA77_004301"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDI6367641.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7922..8746
                     /gene="iclR"
                     /locus_tag="POA77_004302"
     CDS             7922..8746
                     /gene="iclR"
                     /locus_tag="POA77_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDI6367642.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2069 a         2477 c         2305 g         2110 t
ORIGIN      
        1 gcaaacgaat tcccgtacac tgaactctct ggctcactga gggcaccatc atggcactcc
       61 cccgtattac ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa acactgctgg
      121 atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga
      181 aagagtacat cgataaatta atggtcgagc gtgaggctga agcgaaaaaa gcccgccagt
      241 tgaagaaaaa gcaggcttat aaaccggatc ctgaagcgtc atttagctgg tcagcaaata
      301 cgtcgacgcg cggcaggcgc tgattagcgc cccttctttt tacgccccgg agaggtaaaa
      361 cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact
      421 ggacgtttga tgcccgctgt tttcggtttg gccttcactt taggttttgc ctcggaagag
      481 gaattttcga tgagcttaaa gaggtcgatt aactcatcat cagttaaatc gcgccactca
      541 cccagcggaa tgccgcttaa gctgacgttc ataatgcgcg tacgttccag ctttttcact
      601 tcatagccga aatgctcgca catgcgccgg atctgacggt tcagcccctg caccagggta
      661 atgcggaaga caaacggcgc ttcttttttg actttgcact ttttcgtcac cgtcccgagg
      721 atcggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc
      781 accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg
      841 tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaacacg
      901 cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg
      961 acaataccga cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca
     1021 atcaactgac cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc aatggtggct
     1081 cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag
     1141 caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat
     1201 tctcctgtaa aaaacggaat ataccgtacc tttgggttga taaaaaatag tcatgagcga
     1261 actatttgtt atttccattg gcttgaattc gttacctgct caaatcactt tccagaagca
     1321 atgtgcgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcatgcctcc
     1381 cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg
     1441 ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt gcagggtcgc
     1501 cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga ttacacagcg
     1561 aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc
     1621 gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg gaatactttc
     1681 cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga cgtggttaaa
     1741 cgtggcgctc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt
     1801 accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa tctgttcccg
     1861 ttgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt
     1921 gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctaccg
     1981 gaaggtaact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc gaacaccact
     2041 tatgtgttta aggctggtga agaagcggtg ccgctagaag ctggtcaccg tttttaagtg
     2101 aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg cttgacgcgt
     2161 cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat cttcatccgg
     2221 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct gcatatctcc
     2281 cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct gatgcaggcg
     2341 atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aaacgatgtt tgctgcgacg
     2401 caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca gctttaaatt
     2461 actgagcaat tgctcataga gcgcatccag ttccttcaac ccatcaagcg aaaatgcccg
     2521 ccgtgctgcc agcgatttat cggcaatttc gctgcccatg cgctcgacga tatcggaagc
     2581 ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cgacgcgact cttcttctgc
     2641 cagttcctct tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat
     2701 atcatctgcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
     2761 cagcccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gcgcggcgtt
     2821 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
     2881 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
     2941 aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat agatcaccag
     3001 ttccgctttt ggcaatgaca gcttccccat tgtctctgcc agcaaatgga caaacggcag
     3061 gataatcagg ctacccacca gcttaaacag cagactaccc agcgcgacac ggcgggctgc
     3121 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt tagcaccaat
     3181 caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag tcgcagtcag
     3241 cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa tcagcgcatc
     3301 cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat
     3361 cggcgttacg gcctgcacaa tcaactccag cgccagtaaa atcagcccaa gaccaatacc
     3421 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
     3481 aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc
     3541 cgtcccgaca tctgccccca gcacaatgac cagagccggt gcgagggcta ccagatcctg
     3601 agcgacaaac gaggtcacca gcatggtggt ggcattactg ctctgcacca gtgcggtaac
     3661 gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
     3721 caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag
     3781 cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt ctccttatcg
     3841 ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt
     3901 tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctgt ttccagtata
     3961 agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa
     4021 aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc
     4081 aacatcggag acgctcatac ctttacggcg ggcatagtct tcaacctgat cgcgctgaat
     4141 ttgcgccacc gcgtagtact tgctgtccgg gtggctgaaa taccaacccg aaaccgatgc
     4201 accgggccac atggcgaaag attctgtgag tttcatgccg gtatgttttt ccacttccag
     4261 cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc ccggtgccgg
     4321 acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct cgttcggcgc
     4381 atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa
     4441 acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc
     4501 atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc
     4561 tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga atcccgtttt
     4621 ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtgcgcg tttcgtcacg
     4681 gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca cgccacgcgg
     4741 attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg
     4801 ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggccagcga
     4861 ccaggtcata aagaacggtg tccagtcgat gtaattacgc agagtttcga tgctggcttc
     4921 aacttcctgc acgccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc
     4981 gaaatcgtta tcgcgcgccg cttccagcgt gaccggtggt gtgcgcggtt tcttacgccc
     5041 gtgctgaata cgtacggttt cgtactcctt gcgggtacga gcgacaaaat catcacgctg
     5101 agtatcggaa agcagcgcag ccaccacacc aacggtgcgc gatgcgttct gtacatacac
     5161 cgtcgggccg ctgtagttct gctcaatttt caccgccgtg tgcgcttttg aggtcgtcgc
     5221 gccgccaatc aatagcggga tggtgaagcc ctgacgctcc atctctttcg ccacgttaac
     5281 catctcgtcc agcgacggcg tgataagccc cgaaagtcca atcagatcag cattcacttc
     5341 tttagcggta cggagaattt tttccgcagg caccataacg ccgagatcga caatttcgta
     5401 gttgttacat tgcagcacca caccaacgat atttttaccg atgtcgtgga cgtcgccctt
     5461 cacggtggcg atcaccatct tgccgttggt tttgccctgc tctttgctgg cttcaataaa
     5521 cggttcgaga tacgccaccg cctgtttcat gacgcgcgcc gatttgacca cctgtggcag
     5581 gaacattttc ccttcgccaa acagatcgcc gaccacgttc atgccgtcca tcaacgggcc
     5641 ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat cctgctcgat
     5701 aaactcggta atgcctttga ccagcgagta ttccagacgt ttattcactt cccacgagcg
     5761 ccactccgcc tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc
     5821 aagctccagt aaacgctcag tgccatcatc gcgacgatta agaatcacat cttccaccgc
     5881 gtcgcgcagt tcagcgggca ggtcgtcgta aatcgccagc tgcccggcat tgacgatccc
     5941 catatccatg ccattgcgaa tagcgtagta gaggaacact gcgtgaatgg cttcgcgcac
     6001 cggatcgttg ccacggaacg agaaagaaac gttagatacg ccgccggaaa ttagcgcgtg
     6061 cggcagttcg cgtttgatgt cttcacacgc gccgataaag tcctgcgcgt agttgttgtg
     6121 ctcttcaatg ccggtggcaa ccgcgaagat gttcgggtcg aagatgatat cttctggcgg
     6181 gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga ttttccgtgc
     6241 gcgggtatcg gcctgtccct gttcgtcaaa ggccattacc accactgccg caccgtaacg
     6301 acgcaagagc ttcgcgtgat ggataaaggc gtcgacgccc tctttcatcg agatagagtt
     6361 aacaatgcct ttgccctgga tacacttcag acctttttca atgacatccc attttgagga
     6421 gtcgatcata atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg
     6481 caccatcgcc gcttcggcgt cgagcatccc ttcatccatg ttgatatcga taatctgcgc
     6541 accgttttcc acctgctggc gggccacatc cagtgcctcg ctgtatttct cttctttgat
     6601 caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag
     6661 gctatcttcg ccaatgttca gcggctccag gccggacaaa cggcaggcta cggggatttc
     6721 cggcagtttg cgcggcgcta atccttctac tgcacgactc atcgctgcaa tatgttgtgg
     6781 cgtggtgcca cagcagccgc cgacgatatt gagaaaaccc gcttgcgccc attcacgtat
     6841 ctgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc
     6901 gttcgggtgc gcggtgacgt agcattccgc aatccgtgac agctcctgca cgtactggcg
     6961 cagttcatct ggccccagcg cacagttcag gccaaaggtc agagcttcgg cgtggcgcaa
     7021 tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat
     7081 ggtgccggag atcataatcg gcagctcaac gcccagcgct tcaaactccg ttttcaccgc
     7141 aaagaccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagga tcagatccgc
     7201 accaccttcc accagcgctt tggtggactc tcgataagcc gccaccagcc cgtcaaaagt
     7261 gatattacga aatgccggat cgttgacgtc cggagaaata gacgccgtgc ggttggttgg
     7321 gccgagaaca ccggcaacgt agcgcggttt ctccggggtg cgcgcggtcc actcatcagc
     7381 acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg attccatctg
     7441 gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga tatccgcgcc
     7501 cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttactga gtaccagcag
     7561 gtcgttgttg cctttgaggt cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc
     7621 ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg
     7681 ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct
     7741 caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa
     7801 gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact
     7861 attgcattag ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggagactgt
     7921 catggtcgca cccattcccg cgaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc
     7981 gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc
     8041 caatggcagt gtggcactca cggaactggc gcaacaagcc gggttaccca attccacgac
     8101 ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg cgtcaggttg gcgaactggg
     8161 acactgggca atcggcgcac atgcctttat ggtcggcagc agctttctcc agagccgtaa
     8221 tttgttagca attgttcacc ctatcctgcg caatctaatg gaagagtctg gcgaaacggt
     8281 caatatggcg gtgcttgatc aaagcgatca cgaagcgatt attatcgacc aggtacagtg
     8341 tacccacctg atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg
     8401 tgcgggtaaa gcctttttag cccaactgag cgaagaacag gtgacgaagc tgctgcaccg
     8461 caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga
     8521 tctcgcccaa acgcgcaaac ggggttattc atttgacgat gaggaacatg cgctggggct
     8581 acgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa ccgtttgccg caatttcaat
     8641 ttccggaccg atttcacgta ttaccgatga ccgcgtgacc gagtttggcg caatggtgat
     8701 taaagcggcg aaggaagtga cgctggcgta cggtggaatg cgctgacttt tttctggcgg
     8761 gcagaggcaa tattctgccc gtcatatctg agtggcaata gaataagggt gtctgttaat
     8821 cgcattgacg ccaaaataac ttattgtcat acacttcgca cacttttatt aactcagcat
     8881 tatttttaaa catcaaacca cttaattatt aacaaaatcg caagatctca ttacgccgaa
     8941 acttttacgg ctgcattatg a
//
LOCUS       DAMZII010000177         8558 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZII010000177 DAMZII010000000
VERSION     DAMZII010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.177"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(330..1220)
                     /locus_tag="POA77_004303"
     CDS             complement(330..1220)
                     /locus_tag="POA77_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6367643.1"
                     /translation="MFISDETIRIIHLVARHQNITTAAEQLNKVPSTISYTLKKAEET
                     LGVELFIRKGRYIELSPAGSYFIEHSKNILRDLDALKRNTVLIHDGIERELTIAVNNI
                     IPGDFLVSFIQDFERQFTSTTLTVDIEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQIEWDFVVSPLHPLAVKRNPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFAMAISLIEQNVGIGYIPHHLALPLLNRGKLLKKPMREHKHATKLFLAARSD
                     GIGKACLWCMSYLRNKQFMA"
     gene            complement(1298..2521)
                     /locus_tag="POA77_004304"
     CDS             complement(1298..2521)
                     /locus_tag="POA77_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDI6367644.1"
                     /translation="MMRKEDNMNTASGLRITMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQNGIEAPLALLVAAGCVIFVASTAFGFSILGKVAQVAVPVIAL
                     VLCYILYVATHTEVSAPAAIAEMSTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGMTFLVAYPLLYWAGATPSAISGQGSLLNAMAVFGAVMPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLSRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLTQESQVKVLTFAAWITGTAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2544..2933)
                     /locus_tag="POA77_004305"
     CDS             complement(2544..2933)
                     /locus_tag="POA77_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HDI6367645.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGAHVDVEIWQLPLASFAELLMNEPEGLTIGKIKLADGSEVLGVLA
                     ESWLTEGQKEITALGGWRKYTGNFHTM"
     gene            complement(2950..3906)
                     /gene="arcC"
                     /locus_tag="POA77_004306"
     CDS             complement(2950..3906)
                     /gene="arcC"
                     /locus_tag="POA77_004306"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6367646.1"
                     /translation="MVKPLAVVAVGGNALIQDDKRNSIPDQYVAVMESVQHIVDMVEA
                     GWELVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPYDDAFASPSKPIGAFLDEETAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHHTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAIHFGTPQQQWLDAISVYEAQTLLQEGQFGVGSMQPK
                     VEAIIDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3899..5323)
                     /locus_tag="POA77_004307"
     CDS             complement(3899..5323)
                     /locus_tag="POA77_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6367647.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGHAISLNWQPPAQGDID
                     TGLVLASLLRHPLVESANQIAMTRYLEAQPVLVDVMLAKEAIPEMAEQKRILHSGPPI
                     AWEDMCGPVKGAIIGAMLYEGWATSQKDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAASDLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDACKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRELAEIVAKENHHG"
     gene            complement(5320..6879)
                     /gene="fdrA"
                     /locus_tag="POA77_004308"
     CDS             complement(5320..6879)
                     /gene="fdrA"
                     /locus_tag="POA77_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6367648.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALLLAQQRLTSKPDDETDCGIKSPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKYIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETHVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNDQPLPAVENEVSCDDLTMLQ
                     NACQSLPVHRQAIRGVFAGGTFCYEAQLICQQKGFHAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGTSATPVSEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISSLQNAGVIIANSNAQAALWASTVA
                     QTQLQKKELNA"
     gene            complement(6974..7834)
                     /locus_tag="POA77_004309"
     CDS             complement(6974..7834)
                     /locus_tag="POA77_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6367649.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEALPLVFQHYPTIASQRLLFCLEHNVQSTLHLPDSLTHQGLDIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLANHQYRLQKAIEQHAHNTT
                     DISRHYLQRALQGHFSEPICRLLAQLVGSASAMTIASCTEQIMQFGATSGVNCLAGML
                     HGFRTLNTMN"
     gene            complement(7840..8508)
                     /locus_tag="POA77_004310"
     CDS             complement(7840..8508)
                     /locus_tag="POA77_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6367650.1"
                     /translation="MTISIFQAQPFELPFDPCTTALIMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCKEVLAAARQKGIMVIHTREGHREDLSDCPSAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVTGEPVIDKPGKGAFYQTDLHLILQKRGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
BASE COUNT         2130 a         2157 c         2091 g         2180 t
ORIGIN      
        1 aaaatctcat tttatcaaga agaatatcaa attttctccg atacttctac atactccatc
       61 attcatgctc cttttatccg tgcaccgtaa aaccccgtcc ttcagcgggg atataaggcg
      121 ctgttttcca cctaaccagg tgtctgctgc tattcaatgt attggcggat gacggatgcc
      181 gcccgttgaa gcaggacccc tcgtccttag ggcgtggagg atgtcaaaat gaaaacttgt
      241 ggtatgaatc aatccattca tcatcctttc ggatcattgc attcagtata accctggaag
      301 agcctggcaa tatcactgta ttgatggtat tacgccatga attgcttatt tcttaaatac
      361 gacatgcacc acagacaggc ttttcctata ccatcggaac gagcggcaag aaataacttg
      421 gtggcgtgtt tatgttcgcg cattggcttt ttaagcaatt tacctctgtt caataacggc
      481 aatgccagat gatgtggaat ataaccaata ccgacgtttt gttctattaa gctgattgcc
      541 atggcgaagt caggaacaaa aataggtttt tgtccttcca acaaccaggc ttgcattggg
      601 gggaagttaa cggaggtatc ccggatacaa accgcaggat aatgacgcaa ttcactgttt
      661 tctaatggat tccgcttcac tgccagtgga tgaagtgggc tgacgacaaa atcccattct
      721 atttgtccga ccggttcgct aatgatgcct tcactgcttg gtactgcatg aggagcaccg
      781 tagaccagat cagcacgttt actgtacagc gcatcccagc agccgttgta tacttcaata
      841 tctaccgtca gtgttgttga tgtgaattgc cgttcaaaat cttgaataaa tgacaccaga
      901 aaatcaccag gtataatatt gttcaccgca attgtcagtt cacgctcaat accatcgtga
      961 atcagcactg tgttgcgctt taatgcatcc agatcgcgga gaatattttt gctatgttcg
     1021 atgaagtaac tccccgccgg agataattca atatatcgtc ccttacgaat gaacagttca
     1081 acgcccaggg tttcttccgc tttcttcagg gtgtaactga tagtagaagg gactttattt
     1141 aactgttcgg ctgcggtggt gatgttctgg tgtctggcga ccagatgaat aattcgaatc
     1201 gtttcgtctg agatgaacat aaggctccct ggtcagtggt aaagtggctg aatctctcca
     1261 gattcagccg taatcattgc ctgagtatac ccaggaatta gcggtgttga cttgcccgac
     1321 tgagaatcgc atacgcgata catgccacca gaatggagtc aactgaaggg atcgtcgtca
     1381 gggtgaataa gccttttacg gtcataaagc caaccgctgt gcctgtgatc catgcagcga
     1441 atgtcagtac tttaacctgt gactcctgag ttaacaccga ctcttgatag ccattacggc
     1501 gataaaggaa aaagtcagcg atataaattc cggcaactgg gggagtcgcg atacccagga
     1561 acagcagaaa cggaataaac cacgccataa tatccatact gcctacgatg gcggaaagga
     1621 tacccagcgc aacggtaatc tgccatttgg gaaagcggct aagcagtgtg gaaactacca
     1681 acgtcccctg gaacatattg cccgcattac cggtgacgca ggcgaatatc agcaacagtg
     1741 ctgcaggcat aaccgcaccg aatactgcca tagcattcag taaagagccc tggccgctaa
     1801 tggcactcgg cgttgcaccc gcccagtata gcagagggta ggcaaccaga aacgtcatac
     1861 ctgcggcaat cagagcatgt tttcggttat gtacaaaact accgaagtcg ggaagtgtag
     1921 caaccagcac aataatagtg ccaacaacgg tggaaacggc gacacctgta ctcatctccg
     1981 ctatcgctgc tggcgctgat acttcggtat gggttgccac atagagtata taacacagca
     2041 ccagtgcgat aaccggcacg gcgacctggg caactttgcc cagaatggaa aaaccaaagg
     2101 ccgtagaggc gacaaaaatg acacaaccag ctgccactaa cagtgccagt ggcgcttcaa
     2161 tcccattctg cgccagtaag tcatgcaccg aatgaccaaa catattagcg gtaacggcaa
     2221 tccagccgaa cagacttata gccatcaaaa tattaatggc gatggcccct ttttcgccaa
     2281 atgaatattt cacaataccg tatgttggca ggcgggcttt ttctcccaca ctaatggtga
     2341 tggctgaaag taaggtcaga atgacgccgc ctaatagtac gacgctaatt aaactaccac
     2401 tggaaagttg attgccgagg cttgaggatg acaacagcac aggtgtgacg gcaataccca
     2461 gcaaaatcat ggtaatacgc aatcctgatg ccgtattcat attatcttct ttacgcataa
     2521 tatgtttcct ttataaatgc gcgttacatc gtgtgaaaat tcccggtgta tttgcgccag
     2581 ccccccagtg ctgtaatctc cttctggcct tcagtcagcc agctttctgc cagtacgccc
     2641 agtacttcgc taccatcggc aagtttgatt tttccaatgg tcaggccttc gggttcgttc
     2701 attaacagtt cggcaaaact ggccagaggc agttgccaga tttctacgtc tacgtgtgct
     2761 cctccttcat tcacgcggat cattccggga tgacgatcat taatactcca taaccggtaa
     2821 tgggcgtcag ttcggtcttc acggacaaaa ataccgccag ctttttgcat atttgggttg
     2881 agttccagac cgcgcatcag cgttccgtta acggctaaaa gtgttgtagt cattgtgttc
     2941 tccagagaat taaagggtta tccatgttcc gctctgatgt gccagggctg cttttatggt
     3001 ttgcggtgaa gtaatcagtc cgctggcctg tttgccttgt tgctggctgg cattgatgaa
     3061 atcaataatg gcttccactt tgggttgcat actgccgaca ccaaactgac cttcctgcaa
     3121 cagcgtttga gcttcgtaga cgctgatagc gtcgagccat tgttgttgtg gtgtaccaaa
     3181 gtgaatcgcc actttttcta caccggtggg gatcaccagc agatcagcgc ctaactgact
     3241 ggcgagcaat gcggaggcca ggtctttatc gatcacggct tccacacctt tcagttgttg
     3301 cccgtctcgc accacaggta taccgccgcc gccgcaggct ataaccagat atccctggcg
     3361 cacaaggtga gcgatggtgt ggtgctcaat aatttccagt ggcgcaggag agggaactgt
     3421 acgccgccaa ccacgcccgg cgtcctccat cagcgtccag ccgagttgtt gttggcgttg
     3481 ttgtgctgtt tcttcatcaa gaaacgcgcc aatcggttta ctggggctgg cgaaagcatc
     3541 gtcatatggg ctgactcgcg tttgtgtcac cagggcaatt accggtttgt ttatgccacg
     3601 gcgagccaat tcgttatgca gcgctttctg gaacatgtat ccaatggcgc cttgggtatc
     3661 acccacggcg taatccagag gaaccggaga aacttcgtta ctggcaagtt cagagcggcg
     3721 cagaataaag cctacctgcg ggccattacc atgggttagc accagctccc atccggcttc
     3781 aaccatatca acgatatgct gcacgctttc cattactgca acatattgat cgggaatact
     3841 attgcgttta tcgtcctgaa tgagcgcatt gccgccaacc gcgacgacag ccagtggctt
     3901 aaccatgatg gttctccttt gcgacgatct ccgccagttc acgtacagca gcgacaaaac
     3961 aggccatcgg tgcagtggtg atcccggcac caatttgtcc gactcccgct tgtttgtggg
     4021 cgatacccgt attgatgacc ggtaaaatgc cacgatcggt cactttacac gcatcaatac
     4081 ctgctgcggt gggggcaaaa ttgagggctg gaagtgtaaa tgcggggtta ccgccgaggg
     4141 tgattgcctg catccgacgg ctgttattgg tggcatcggc aggggtgcct cccacaaatt
     4201 taacaatcgc cggagatgac gccatcgcga agccacccac acctgcggtt tcagtaattg
     4261 cgctatcacc caggtcagaa gcggcgtcgt caacgccaaa tccggggaag aacagacctt
     4321 caactgcatt ggcaggagcc tgaaaccagc gatcgccagt accggaaagc cgaataccaa
     4381 aattcacccc gttacgcgcc atcaccgtaa ccattgaact gaacggtaca ttggcggcgg
     4441 catccatcat tgctttacag gcagccatcg acaaattgag gaagaagtga tcgttacctg
     4501 tgataaatgc cacgacgcgc tggatatgtt cctgtggaag agagcaggcc agcaatgccg
     4561 ggagcaggcg cttaatcagc aaaccggtag cggcagcatt gcggttatga acttcatccc
     4621 ccatatgcag cgcctgcgcc attaatggct tgagttccag ctcgccgtgt tgtgcgattg
     4681 ctgctttcat ggccggcgcc agttcatcac gcatccaggc cagacgattg agaacttcat
     4741 cgttattggc accaaaacgg agaaccttac ccagaccctc attgaaattg ctaaaagtac
     4801 gatggccgtt ggttttgttt tccaccaccc ataatggcat cgacgggctg ataatgcctg
     4861 ccattggtcc aaccgcgtga taatgatgac aaggagcaag gtcgatttcg cctgcgttta
     4921 tctggttttc tgcgtctttc tggcttgtag cccagccttc gtacagcatg gcaccaataa
     4981 tcgccccttt taccggacca cacatatctt cccaggcaat cggtggacca gagtgtaaaa
     5041 tgcgtttttg ttcagccatt tcggggatgg cttctttagc gagcatgaca tccaccaaca
     5101 cgggctgtgc ttcgagataa cgtgtcatgg cgatttggtt ggcactttct accagtggat
     5161 ggcgtagtaa tgacgccaga accaaaccgg tgtcaatatc accttgtgct ggcggctgcc
     5221 agttgaggga tatagcatgc cctcctgcat gttgaatatt gtcagcaaaa ctgtgtagcc
     5281 ccgcgttcac gaccttcaaa ggctgattaa ataaggtatt catgcattta gctccttttt
     5341 ctgcaactga gtctgtgcaa cggtgctggc ccatagcgcc gcctgtgcat tgctgttggc
     5401 aatgatgacg cctgcatttt gtagtgagct tatctgctga ctgcgtattt gcggatcagc
     5461 ttctgttccg caaacgtggg caatcagcaa cggtgcatta ttcatgtcga tgtgtgaaag
     5521 ttggtccagt aattcgctga caggcgtagc ggaggtgcca taaccaagta caaggtcgaa
     5581 taacacaacg gcagtatgtg agtcgttgag ttcgttgagc agacgttgat tgcgcagagt
     5641 cgggtcgatc attggatgcg gtttgccacg ggtgaaatca tcgtccccca tatcaatcag
     5701 tgtatgatct tcactttgcc agatatttgc cagagcacga ttacccgcaa ctggcgtatt
     5761 ggaggcggca tggaatccct tttgttggca gataagctga gcttcgtaac agaaggttcc
     5821 tccggcgaaa acaccgcgta tcgcctggcg atggactggc agactttggc aggcgttttg
     5881 taacattgtg agatcgtcgc aggaaacctc gttctcgaca gccggaagtg gctgatcatt
     5941 cagtaacgcc acagcgatgt cggcggcgct ggcaagtgtg gtggcggcag tgatattggg
     6001 acgtgccaga tcatgcggat tcgccccgag gaaattcacc accacgggtt ttccacaagc
     6061 ctcggcgcgc tccagaatag tccgggcaac gatcggtgaa ggcggttttg aaataagcac
     6121 aatcacgtga gtttcatcgt cttgtgccag cgcatctaaa gcaaagagca ttgatattcc
     6181 accaatctct tcactgagat catgtccgcc tgttcccaac gcctgagaaa tacccgctcc
     6241 gagctggtcg attcggcaag tcacttcctg tagcccggtg cctgacgcgc caataacacc
     6301 aattgctccg cgtttcacca cattagcgaa gcctaacgga atgccgttaa caatggcggt
     6361 tccacagtca ggtcccatta cgatgcgctg acgttctcgg gcaagcgttt tgatgtattt
     6421 ttcctgcccg atactgacgt tatcgctgaa catcatcacg ttcattccga ggtttaacgc
     6481 tttaattgct tcggcggcgg cgtagtctcc cggtacagaa atgagtgcca ggttggcttc
     6541 gggttcagct tccagtgcca tttccaggct gaccttttcc ggtgatttaa tgccacaatc
     6601 cgtttcatca tcgggtttgc tggtcagacg ttgctgggcc agtagcagtg cttcattgca
     6661 aatatcttct tcacccatga ctgcaatcac taaatcattg ggactggcgt tgatttcgtt
     6721 acccagaccc gcatcgcgca attgctcgag attattgggt gttcccatga cgaccgaggc
     6781 ctgttgaata ccgggcaact tgctgatttg cgcggaaatc tgcatcagag aaactgagtc
     6841 ctgataaaga ttactgaaaa ctttatattt aactttcatg ctgttacgcc tgttccattc
     6901 cgcccgatag caaaattcgg gcaaagcatc cccgtgtagc cccataaacg ggctacggga
     6961 gaaaaggttg aaatcaattc atggtgttca gggttcgaaa tccatgcagc attcctgcaa
     7021 ggcaatttac tcctgatgtt gcgccaaatt gcatgatctg ttctgtacag gatgcgattg
     7081 tcattgccga tgcgctccca accagttgtg cgagtaaccg gcaaatcggt tctgaaaaat
     7141 gtccctgaag cgcacgttgc agataatgac gacttatatc cgtggtgttg tgtgcatgtt
     7201 gttcaattgc cttctgcaac ctgtattgat gattggcaag tggtgcaggg agttgccata
     7261 accagagggc tgccagatag cccagcaaat agtcatctcc atcgggcgtt aagcctttac
     7321 caaagccgat cagttggggt acctggcgtt ccagcgcatc tggatgttcc ataatatcta
     7381 atccctggtg cgtgagacta tccggcaggt gcaatgtgct ttgaacgtta tgctccagac
     7441 aaaataaaag ccgttgcgat gctatcgtgg ggtagtgctg gaaaaccaaa ggcaatgcct
     7501 caagagtcag cattcccccc acaaatggcg actgccagag cgttgcgtta tcgagcgcca
     7561 cacaaaatcg aggtgtccgc agtaagcctg gggttaattg aatgggatct gaatgggcaa
     7621 tctctcttcg ccagtcccaa cattcaggta gccagactct ggcagcatca gggagattca
     7681 gatacgtttg cgcacacaac agagtgatga atgttcccgc gtctgaatac agatttaccg
     7741 catgtttaaa agcgctatgt agacgtaaat cttgcgtttc attctgcggg aaaagatatc
     7801 ccaacgacga tgcacaaagc gtaacctgta ccatcggcgt tagccttcca gacctgcaat
     7861 aatggctttg gaatcagtaa cccaaccgaa aatggcacct tgtgctttga tcatctcaag
     7921 ggcgtatttc tggaattccg ggaaatagga gccaacgcaa tcttccggta tgatgcattc
     7981 atagccacgg tcatttgctt cacgcacggt ggtggttacg caaacttcgg tagtcacgcc
     8041 acaaacaata agtgtcttga taccacgctt ttgcaaaatc agatgtaagt cggtttgata
     8101 aaaagcgcct ttaccgggtt tatcaatcac aggttcgcct gtcaccggat acagttcagg
     8161 aatgatgtca tgacctgctt cacccctgac cagaatgcgt cccatcggtc ctggttcacc
     8221 aataaaggtt tttccgccgc gcgtcagttt ggctgatggg cagtcgctta agtcttcacg
     8281 atggccttca cgggtatgaa taaccataat tcctttttga cgtgcggctg ccagaacttc
     8341 tttacatggt gcgatagccg tgcgaaccag tgaaacatca ttacccaatg cttctccaaa
     8401 ccctcctgct tcgacaaaat cacgttgcat gtctatcatg atcagtgccg ttgtgcacgg
     8461 atcaaagggt aattcaaagg gttgagcctg aaaaatggat atcgtcatga caaatctcca
     8521 gtatatattg tttactggtg acgagtattc aatataaa
//
LOCUS       DAMZII010000178         8507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZII010000178 DAMZII010000000
VERSION     DAMZII010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.178"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(133..209)
                     /locus_tag="POA77_004311"
     tRNA            complement(133..209)
                     /locus_tag="POA77_004311"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Asp,seq:gtc)
     gene            complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="POA77_004312"
     CDS             complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="POA77_004312"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDI6367651.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1138..1605
                     /gene="rnhA"
                     /locus_tag="POA77_004313"
     CDS             1138..1605
                     /gene="rnhA"
                     /locus_tag="POA77_004313"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDI6367652.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1602..2324)
                     /locus_tag="POA77_004314"
     CDS             complement(1602..2324)
                     /locus_tag="POA77_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6367653.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2358..3113
                     /gene="gloB"
                     /locus_tag="POA77_004315"
     CDS             2358..3113
                     /gene="gloB"
                     /locus_tag="POA77_004315"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDI6367654.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLKAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPPETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3185..4543
                     /gene="mltD"
                     /locus_tag="POA77_004316"
     CDS             3185..4543
                     /gene="mltD"
                     /locus_tag="POA77_004316"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDI6367655.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4591..5361)
                     /locus_tag="POA77_004317"
     CDS             complement(4591..5361)
                     /locus_tag="POA77_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI6367656.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DTSVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5439..6239)
                     /locus_tag="POA77_004318"
     CDS             complement(5439..6239)
                     /locus_tag="POA77_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI6367657.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6480..7394
                     /locus_tag="POA77_004319"
     CDS             6480..7394
                     /locus_tag="POA77_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6367658.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7391..8194)
                     /gene="dkgB"
                     /locus_tag="POA77_004320"
                     /pseudo
     CDS             complement(7391..8194)
                     /gene="dkgB"
                     /locus_tag="POA77_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
     gene            complement(8357..8433)
                     /locus_tag="POA77_004321"
     tRNA            complement(8357..8433)
                     /locus_tag="POA77_004321"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8397..8399),aa:Asp,seq:gtc)
BASE COUNT         2258 a         2050 c         2066 g         2133 t
ORIGIN      
        1 agacaacaag cgagaatcac gcacttacgc cagaataaaa tcattgaaca gagaaatggt
       61 ggaattcaaa tacaaaaaaa ccgcaaaatt aaaaatcttg cggctctctg aactcatttt
      121 catgagtgaa tttggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
      181 ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata
      241 ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa
      301 aaatcgccca agccgctatt tttagcgcct tttacaggta tttatgcccg ccagaggcag
      361 cttccgccct tcttctccac cagatcaaga cgggcttcat gagctgcaag ctcttcatct
      421 gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca
      481 ccttgttgct gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccggtc
      541 atcgccagat aaacttccgc aaggatctgt gcatcgagta atgccccgtg tagcgttcgt
      601 ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg cttaccagga
      661 aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga
      721 atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa cgctgcgtta
      781 tggatcacca actccgcgcc gcggatatag tccatgaact catcggctac ttcggcaaac
      841 gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc
      901 accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc
      961 acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcacc aatctggttc
     1021 ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc
     1081 atttatgtca gacttgtcgt tttacagttc gattcaatta caggaagtct accagagatg
     1141 cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gtaatcctgg acctgggggt
     1201 tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc
     1261 accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa
     1321 cattgcgaag tcattttgag taccgacagc cagtatgtcc gccaggggat cactcagtgg
     1381 atccataact ggaaaaaacg tggctggaaa acagcagaca aaaaaccagt aaaaaatgtc
     1441 gatctctggc aacgtcttga tgctgcattg gggcagcatc aaatcaaatg ggaatgggtt
     1501 aaaggccatg ccggacaccc ggaaaacgaa cgttgcgatg aactggctcg tgccgcagcg
     1561 atgaatccca cactggaaga tacgggctat caagtcgaag tttaagcctg tggtttacga
     1621 cattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact ctgtttcatc
     1681 gggtttagcg ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc
     1741 gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt tccacgggag aacgtggaaa
     1801 cggctggcgt gcagcacttc aaaattcaac aaagagagcc agtccagttg ccgcatcaga
     1861 gtaaacatcc ggctgttata gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat
     1921 cccattaaac tgatgggatt gaagccacta atcaccagcc agccatcatc aatcaatacc
     1981 cgatcggctt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt atgtgccagt
     2041 agacaaacat caacggattt atcggcaaaa ggaagatgaa gtgggtccgc ctgtacctgg
     2101 acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg
     2161 atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac
     2221 gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag
     2281 caatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc
     2341 acactgagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct
     2401 gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac gcagagccgg
     2461 tattaaaagc cattgccgcc aataactggc aaccagaggc catatttctc acccaccatc
     2521 accacgatca cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt
     2581 atggtccacc agagacacaa gataagggaa caacacaggt agtcaaagat ggcgaaactg
     2641 ccttcgtttt ggggcatgaa tttagtgtaa ttgccacgcc gggtcacact ttaggacata
     2701 tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg
     2761 gtcggttgtt tgaagggaca gcatcacaaa tgtatcaatc acttaaaaag ttaagtgcgt
     2821 tacctgacga tacattggta tgttgtgctc atgaatatac gttatcaaat atgaagtttg
     2881 ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt
     2941 tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg caaattaatg
     3001 tttttttaag aacggaagat attgatttaa ttaatgtaat taatgaagaa acattattgc
     3061 aacaacctga agagcgtttt gcatggttaa ggtcaaagaa agataggttc tgataaaact
     3121 ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa ctattgacac
     3181 acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt gccagagtac
     3241 cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg aagcagcaaa
     3301 gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag atggtgactt
     3361 gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc ggattcgcga
     3421 acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt tacgggcaga
     3481 gccgtatatg tactggatag ccgggcaagt taaaaaacgt aacatgccta tggaactggt
     3541 actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg ccaatgccgc
     3601 aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac agacccgcaa
     3661 ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca tgatgcagcg
     3721 tctgaacaag atgtttgacg gcgactggct tctgaccgta gcggcttata acagcggcga
     3781 aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg
     3841 gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg cattgagtga
     3901 tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg aaagccgtgc
     3961 tctggcgcgt gtgcacctga gcagcccggt tgaaatggcg aaggttgcag atatggcggg
     4021 gatttccgtc agcaagctga agacattcaa cgctggcgtg aaaggctcca cgctgggcgc
     4081 aagtggtccg caatacgtga tggtgccaaa gaagcatgca gatcaactgc gtgaatctct
     4141 cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata cgccgcttaa
     4201 cagccgtgtt tacaccgtac gctctggcga cacgctttca agtatcgctt cacgtctcgg
     4261 cgtaagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc tgaagccagg
     4321 ccaaagtttg acgattggtg caggcagtag cgcacagcga ctggcaaaca acagcgatag
     4381 cattacgtat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac gccacggcgt
     4441 gaacatcaaa gatgtgatgc gctggaacag cgacactgcg aatctgcaac caggcgataa
     4501 gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaacca gataataaaa
     4561 aggcaccgat tcccccggtg ccttttttat ttatgctgct ttatgtgctt ctaccatgat
     4621 gatatcactg gtgaaagagc catcattctg caaggcaaaa tacgttctca cctctgtcga
     4681 tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac gcattctcgc
     4741 gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa ctatcagatt
     4801 ggcttcattg attaacctca accactcacc gctggcgtag tttcgtacgt gagaggtatc
     4861 gcgtaatgct tctaccgtct gtaaccagat gtcgcgcact ggatgacccg gagacattac
     4921 gtccatcaca atcatcctac cgccaggttt caatatccta ttcacttctc gcagtgctgc
     4981 accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa atgcgttatc
     5041 ggcaaatggc agactttcgg cataaccctg gcgggtggtg atatttttca gttgccgggc
     5101 ttcggcagct tgtgccacga catccagcat ttgggcagat aagtcatacg ccaccaccgc
     5161 gctcacgttc tgcgcagcga caaagctggc atgccctgct ccgcagccca tatcaagtac
     5221 acttgtatca ggataatcag ccagacgcac cgccaggcgc tgtaaatctc gcccggatgc
     5281 atgtacggta ctggttaaat actcactggc ctgcgagcta aactgctttt ctacgtggtc
     5341 atggtgggat tgtgttgtca ttgcgctgtc cttttgttat ttgagtataa aagggcagcg
     5401 ccaaactttg gtctgccctg tggcagacct gacatacctt atttatcagg tttgccggga
     5461 ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttcg
     5521 ctgacgttca gaccacggta gaaaacaaaa tcgagcggac ggccaaacgc ccgacggcgc
     5581 tgatcgtcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg
     5641 ttcatccttc tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtga
     5701 tgagctatct gatcgccaat aggaagtaac tgcttactat aaacatccac gcccagactg
     5761 aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tatcaggtaa tggatagacc
     5821 gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa
     5881 tacactggat gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagtacgaaa
     5941 gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtactaa ctctggcgtt
     6001 gtctgcgctt cctgcaataa caccagatgt gcatctttgc cgtagttctt taataccgac
     6061 aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg
     6121 gtacttaacg gttccccagg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt
     6181 aagatccttt ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact
     6241 tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactgttat
     6301 agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta
     6361 caccggtcag aattttgctc attgttgaga acattaaatc ggatgacata tcacgcatcc
     6421 tgatggttta atgacaaata aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa
     6481 tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta
     6541 gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggca gtgaaaaagc
     6601 tggagatgaa acttggcgtt agcctgctta atcggaccac gcgacaactt agcctgacgg
     6661 aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag
     6721 aatcagaaat tatggagacg cgtaatacac cgcgtggact gttgcgcatc gatgccgcaa
     6781 ctccagtggt gctgcacttt ctgatgccgt tgattaagcc tttccgtgaa cgctatccgg
     6841 aagtcacttt gtcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagttg
     6901 atgtcgcgat acgcgccggt acgttaacgg attccagctt acgtgccagg ccgttattta
     6961 atagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa
     7021 cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata
     7081 cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt ttgtcatcca
     7141 atagtgggga aacactaaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg
     7201 actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag
     7261 tgttgccagt ggcaatgcca ttcagtgcag tctattacag cgaccgtgcg gtaagtacgc
     7321 gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg
     7381 tcagagaggc ttaatcccat tcaggagcca gacctttcgg gctaaccagg cggtcgttgc
     7441 aatccagggc ggcgatcgct tttttatctt tggcatcgag ctgtaaattt tatgccttaa
     7501 gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca
     7561 tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgcg atacgagcaa
     7621 taacctcatc tttcagggcc ttaccatacg ccagcgtcat ataggaagta atatggatgc
     7681 cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag agttcaatct
     7741 ggttagtagc gatgttttca gcgccaacag cagcaatcgc cttttccatc aatgggatcg
     7801 tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggctttc agcagcgcct
     7861 gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat
     7921 caacataatc ggtacgcaat ttttgcaggc tctctttcag acttgggatc aatttgtctt
     7981 tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac
     8041 tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg gtatcaattg
     8101 cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttcagac
     8161 ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc
     8221 gttaactgag gtgattatgg gcttcagact gttgaggaaa aagagcgtaa aatgcagagg
     8281 atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga gcgttaactc
     8341 gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
     8401 ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt
     8461 tatgaccggc ttactgctta atttgatgcc tggcagttcc ctactct
//
LOCUS       DAMZII010000179         8500 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZII010000179 DAMZII010000000
VERSION     DAMZII010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.179"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..660)
                     /locus_tag="POA77_004322"
     CDS             complement(<1..660)
                     /locus_tag="POA77_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6367659.1"
                     /translation="MSQPNPLCVGIDVSKTTLDIATANNEFAQFTAGNDTDGFELIIA
                     ELRKHSVALVLMEATGGLEAAVACSLQAEGFDIAVINPRQARNFSRAMGYLAKTDRID
                     AKVLSQMAEVINRHPERERFIKAIPDAERQILAAMVVRRRQLITMLVAERNRLHPAHP
                     QNRKSINTIIKALEDELSRIDEAMNNHIKTHFKELAARLSSIKGVGAMTVATLLSEVP
                     EL"
     gene            981..1187
                     /locus_tag="POA77_004323"
     CDS             981..1187
                     /locus_tag="POA77_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6367660.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            1213..1752
                     /locus_tag="POA77_004324"
     CDS             1213..1752
                     /locus_tag="POA77_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6367661.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQSQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            1810..2043
                     /locus_tag="POA77_004325"
     CDS             1810..2043
                     /locus_tag="POA77_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDI6367662.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            2102..4066
                     /locus_tag="POA77_004326"
     CDS             2102..4066
                     /locus_tag="POA77_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6367663.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWLVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKENTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALGAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTGQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLILLAWTLCLNVFGSGAYSNPAKIRLECEHYALTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDNQAEAA"
     gene            4135..4569
                     /gene="psiB"
                     /locus_tag="POA77_004327"
     CDS             4135..4569
                     /gene="psiB"
                     /locus_tag="POA77_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDI6367664.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGTDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            4566..5285
                     /locus_tag="POA77_004328"
     CDS             4566..5285
                     /locus_tag="POA77_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDI6367665.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWLWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            5565..5723
                     /locus_tag="POA77_004329"
     CDS             5565..5723
                     /locus_tag="POA77_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI6367666.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAA
                     FMAYESGK"
     gene            6349..6645
                     /locus_tag="POA77_004330"
     CDS             6349..6645
                     /locus_tag="POA77_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367667.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNNAVKASLAYQSNQDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            6756..7577
                     /locus_tag="POA77_004331"
     CDS             6756..7577
                     /locus_tag="POA77_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDI6367668.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGNINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(7874..8476)
                     /locus_tag="POA77_004332"
     CDS             complement(7874..8476)
                     /locus_tag="POA77_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI6367669.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT         2025 a         2124 c         2395 g         1956 t
ORIGIN      
        1 cagttcaggt acttcagaga gcaatgtggc aactgtcatt gcacctaccc ccttaatgct
       61 gctcaaacga gctgcaagct ctttgaaatg agttttaatg tgattattca tcgcctcgtc
      121 aattcgggaa agttcatcct ccagggcttt tatgatggta ttgatactct ttctgttctg
      181 aggatgtgca gggtgaagtc tgttccgttc cgcaaccaac attgttatca actggcgtct
      241 acgtaccacc atagcggcaa gaatttgccg ttcggcgtcg ggtatagctt tgataaagcg
      301 ctcccgttct ggatgacggt ttattacctc ggccatttga gacaacactt ttgcatcaat
      361 cctgtcggtc tttgccagat atcccatagc acgagagaaa ttcctggcct gtctgggatt
      421 gatgaccgcg atatcaaaac cttcagcctg aagtgaacag gctacagcag cttcaagccc
      481 acctgtagcc tccatcaaaa ccagcgccac cgaatgcttt ctcaattcag caatgatgag
      541 ctcaaaacca tcggtgtcgt taccagcagt aaactgagca aactcgttgt tagcagtagc
      601 aatgtccaga gttgttttag aaacatcaat acccacacaa agcggatttg gctgactcat
      661 tattacccat tcttgcaaat gcgttatgaa ttacggacaa ctgttcgggt ttcagatgag
      721 tggctcagcg tatgcgccat ttgcttacat acgggcttaa accctaaggg gcaccaggct
      781 gcatacgctt tgccaaatct attcgctgct catgataacg aagaaccaag atacaagggg
      841 caacgccgtt tcgcaaaccg cgcttcacgt cgttgcggcg ggttcctgcc cgttgttctg
      901 tccggtgcca ttcacttgtt taagcccctg tcgccggggg caaagacagc cacaaccgtc
      961 tccgtcagtc caggggtgaa atgcacatcc cgcaaaactt tttgcccaga agcaggcaaa
     1021 aacttttgcg gtctgccccc ggacagtcgt ttccggccgt ggcagtgatg ccccttgcgg
     1081 cgaaggttct caaaaaggtg atggtgaata ccggagggaa caaaaggcag gtccccgcaa
     1141 aacgtcaaaa ccgcggctcc cgaaactcaa aagatgatgg gggctgaaaa catcaacaga
     1201 aggaggcaca tcatggcagt tcgtggcatt aacaaggtca ttctcgtagg acgcctggga
     1261 aaagatccgg aagtccgtta catccccaac gggggcgcag tggcaaacct gcaggtcgcc
     1321 acgtcggaaa cttggcgtga caaacagaca ggggagatgc gggagcagac agaatggcat
     1381 cgcgtggtgc tgttcggcaa gctcgcggaa gtggctggcg aatatctgcg caagggcgcg
     1441 caggtctaca tcgaaggtca gcttcgcacc cgtagctggg aggataacgg tataacccgt
     1501 tacgtcaccg aaattcttgt taagaccacg ggcaccatgc agatgctggg acgtgccgca
     1561 ggtgctcaga ctcagccgga agaggggcaa cagttcagcg gtcagtctca gccggaacca
     1621 caggcggagg ccggtacgaa aaaaggaggc gcaaaaacga aaggccgtgg acgtaaggcc
     1681 gcgcagcccg agcctcagcc gcaaccgccg gagggggagg attacgggtt ttcagatgat
     1741 atcccgttct gactggcctg actgtgacaa ccgccccgcc ctgcgcgggg catcaccgga
     1801 gatatgagga tgagcgaata tttcagaata cttcagggac tgcctgacgg tccttttacc
     1861 cgcaaacatg ccgaagccgt tgccgcacag taccggaacg tctttatcga ggatgaccac
     1921 ggagaacatt ttcgcctggt tgtccgtaat aacggaacaa tggtctggcg tacctggaat
     1981 tttgaggacg gtgccgggta ctggatgaat catgccatca gggatttcgg gattcttaag
     2041 taagagaggt gccggacgcg cgaacgtccg gcaggacata agcaattata aggggatgat
     2101 tatgcctgta acgaagtgtg aaccagaaac cacccgcaaa gcaagccgta aatctgcaaa
     2161 aacgcaagaa acagtcctgt ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct
     2221 ggcctcgctg attaagtcac cactgaatgt gcgcacggtg ccgtattctg tggagtccgt
     2281 cagcgaactg gcggagtcca ttaaaggcgt cggcctgttg cagaatctgg tcgtgcatac
     2341 cctgcctggt gaccgttacg gtgtcgccgc aggtggtcgc cggctggctg cactcaacat
     2401 gctggcagag cgtggcatca ttccggctga ctggcttgtc cgtgtgaagg tcattccgca
     2461 ggagctggcg acggcggcat caatgactga aaacggtcat cgtcgggata tgcaccctgc
     2521 tgaacagatt gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg
     2581 tgatttgctg ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc
     2641 cccggttatc ctcgatgcgc tggcagaaga ccgcatcacc accgagcact gtcaggcgct
     2701 ggcgctggag aacgacaccg cgcgtcaggt gcaggtgttt gaagcggcct gccagtcagg
     2761 atggggcggt aaaccggatg tgcgggttat ccgcaatctg attacggaaa gtgaagtggc
     2821 ggtgaaggaa aacactaaat tccgcttcgt gggggctgat gctttctcac ctgacgaact
     2881 gcgtactgat ttgttcagcg atgacgaggg tggctatgtg gactgcgtgg cgctcggtgc
     2941 cgctctgctg gaaaaactcc aggctgtcgc tgaacacctt cgggaagccg aaggctggga
     3001 atggtgcgcc ggacgcatgg agcctgtcgg tttctgccgt gaggatgccg gaacataccg
     3061 ctgtctgccg gagccggaag cggtgctgac ggaggcagaa gaagaacgcc tgaacgaact
     3121 gatggcacgt tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga
     3181 aatgaagctg attgactgca tggcgaaggt cagggcgtgg acgccggaga tgcgtgccgg
     3241 aagtggcgtg gtggtgtcct ggcggtatgg aaacgtatgt gtccagcgtg gtgtgcagtt
     3301 acgcagtgag gatgatgcgg ctgacgacgc tgaccgcacg ggacaggtgc aggaaaaagc
     3361 gtcagtggag gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc
     3421 agtccaggcg gcactgatgc agcaaccgga caaatccctg atactgctgg catggacgct
     3481 ctgcctgaat gtgtttggca gcggggcgta cagcaatcct gcaaaaattc gtctggaatg
     3541 tgagcattat gcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc
     3601 gctgatggca gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat
     3661 gacgacattc ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc
     3721 atgcagcctt aacggtgtcc agacccgtga gtgtggtcac acgtcacgca gtccgcttga
     3781 ctcgctggaa accgctatcg gattccacat gcgcgactgg tggcagccga caaaagcaaa
     3841 ctttttcgga cacctgaaaa agccgcagat tatcgatgcc ctgaatgatg ccggactgtc
     3901 cggtgctgcg cgggacgcgg agaaaatgaa gaaaggcgat gcggctgaac atgcagagtt
     3961 ccacatgaaa gacaaccgct gggtgcctgg ctggatgtgt acaccacgtc cacaggcaga
     4021 gacggaaacc acggagtacc gtgacaacca ggcagaagcc gcctgaccgc cagtcataaa
     4081 tcatttatca cgccgccccg ccggagacgg ggcggcagca agggagatac cgtgatgaaa
     4141 actgaactga ccctgaatgt attacagacc atgagcgcac aggaatatga agatatccgg
     4201 gctgccggaa ctgatgaacg tcgtgagctg acacacgccg tgatgcggga gctggatgcg
     4261 ccggacaact ggacgatgaa cggcgagtac ggcagcgagt ttggcggatt tttccccgtt
     4321 caggtccgtt tcacgcctgc ccacgaacgt tttcacctgg cgttatgttc accgggcgat
     4381 gtctctcagg tctgggtgct ggttctggtg aatgccggtg gtgagccgtt tgcggtggtt
     4441 caggtacagc ggcggtttgc accggaagcc gtcagccatt cactggcact ggcggcgtca
     4501 ctggacgcgc aggggtacag cgtcagtgac atcatccata tcctgatggc ggaaggaggt
     4561 caggcatgag cgcacgttca caggcactgg ttcctctcag cacagagcaa caggccgcat
     4621 ggcgggcggt ggcggagacc gaaaaacgcc gccatcaggg taacacgctg gctgaatatc
     4681 cgtatgcggg ggcgtttttc cgctgtctga acggaagccg caggatatcg ctgtctgacc
     4741 tgcgattttt catgccgtcc ctgaccgcag aagaactgca cggcaaccgt cttcagtggc
     4801 tgtatgccat cgatgtgctg attgaaacac agggcgaggt ttgcctcctt ccccttccag
     4861 gcgatgcagc agaacgcttg ttcccgtcgg tgcggtttcg tgtcagggaa cgcagtcgcc
     4921 ataaatccgc gctggtcatg cagaagtaca gccgccagca ggcgagggag gcagagcaga
     4981 aagcccgtgc gtaccaggcg ctggtcgctc aggctgaaat cgaactggca ttccattcgc
     5041 cggaaacagt cggaagctgg tatgcacgct ggagtgaccg ggtggcagag catgacctgg
     5101 aaaccctgtt ctggctgtgg ggtgagcgtt ttccgtcact ggccggaatg gaacgctggc
     5161 agtggcagga tatgcctttc tggcaggtca tcgcagaggc tggtatggcg gcaagggagg
     5221 ccggtcatgc cgtccgtgag atggagcggt ggatggtgcc gaacaaactc cgggaggcag
     5281 cgtgatgcgg caacaatcac acagattacc cgtaaacagc ctgaatgagc ggattatttt
     5341 caggaaaagt gagtgtggtc agcgtgcagg gatatgggct atgatgtgcc cggcgcttga
     5401 ggctttctgc ctcatgacgt gaaggtggtt tgttaccgtg ttgtgtggca gaaagaagat
     5461 agccccgtag taagttaatt ttcattaacc accacgaggc atccctatgt ctagtccaca
     5521 tcaggatagc ctcttaccgc gctttgcgca aggagaagaa ggccatgaaa ctaccacgca
     5581 gctctcttgt ctggtgtgtg ttgatcgtgt gtctcacact gttgatattc acttatctga
     5641 cacgaaaatc gctgtgcgag attcgttaca gagacggata cagggaggtg gcggctttca
     5701 tggcttacga atccggtaag tagcaacctg gaggcgggcg caggcccgcc ttttcagggc
     5761 tgatgctggc ctgactgcac tgaagcgcct ttataaaggg gctgctggtt cgccggtggc
     5821 ccctttctcc ttgctggcat tgtaagggca tgaacaacca gactgccagg aaggccgatt
     5881 ccctccggga acggggatga acgtgcgcct gcggcgctcg ttcttttccc ccgccttctc
     5941 tgtttatgac ctgccggaaa tcacatcacc ttccctgtga ttattctctt tttcctcctg
     6001 ttctgactct gattactgct gctctgatta ctgtctgttt tgttgttctc ctgcttatgc
     6061 cttttccggg cgtccccctg gcggggcggg ctgtcgtgaa cggagccagc gaagctgtct
     6121 ccgccctggc gggcttccat ccctgacgca aaaaaccggg tttgcagcga ccggaacggt
     6181 cgcgccaccc ttgtcgcgct gtggcctgcg ctcccggcat cccggcaaaa agacgccggg
     6241 atgtaaggag caacgccgtt tcgcaaaccg cgcttcacgt cgttgcggcg ggtcccgaaa
     6301 ctcaacagat gatgggggct gaaaacatca gacacaggag aatgaaacat gagcgcattt
     6361 tcttttaaca ccacttatca gccaacaggc gatcagcaga aagacgcaat cgcacaaatc
     6421 gggatcatgc agaataacgc tgttaaggcc agtctcgcct atcagagcaa ccaggattca
     6481 ggggcgcttt ttaaggtgct ccaccaggtc aataatgagc tgcatgactt actggatagc
     6541 ctggagaatc atacccctct tgtcagaaaa aacgctgatg agctgatcgc gctgttactg
     6601 cttttcaccc ggcaggttgg tcagtcccga actgacctga tttgatggtc ctttccctcg
     6661 gagttgttat cttattgtta acagtaaccg agggaggatg atattacgcg aaaaaatttt
     6721 tattaatttt ataccttcat aaaggagtta ctgttatgcg attagcttcc cgttttggtc
     6781 ggtataattc cattcgccgt gagcgtcctt taacggatga tgaattaatg cagtttgtgc
     6841 cgtctgtctt ttccggtgat aaacatgaat cccggagtga gcgttataca tatattccaa
     6901 ccattaatat catcaataag ttacgtgatg aaggtttcca gccattcttt gcctgtcaga
     6961 gtcgggttcg cgatttggga cgccgcgaat acagtaaaca tatgttacgt cttcgcaggg
     7021 aagggaatat taacgggaaa gaagttcctg aaattatcct gcttaattca catgatggtt
     7081 catccagtta tcagatgatc cccggaattt ttcgttttgt ctgcacaaat ggcctggtgt
     7141 gcggaaataa ttttggcgaa atccgcgttc cacataaagg tgatattgtc gggcaggtta
     7201 tcgagggagc gtatgaagtg ctcggtgtct ttgataaggt cactgataat atggaggcga
     7261 tgaaagaaat tcatcttaac agtgacgagc aacatttatt tggcagagct gcactgatgg
     7321 tcaggtatga agatgaaaat aaaacgccag tgacacctga acagataatt actccccgtc
     7381 gtcgggaaga caaacagaac gatctctgga caacctggca gcgggttcag gagaatatga
     7441 taaaaggtgg attatcgggg cgaagtgcct ccgggaaaaa taccaggaca agagccatta
     7501 caggtattga tggtgatata agaatcaaca aggcgttatg ggtgattgcc gaacagttca
     7561 gaaagtggaa gtcatgataa tataagtaaa gccccgaaaa aattttcggg gctttactta
     7621 tattatcatt tttcggcatt gctgtgtcgt ctgatatacc tcaatttcat caaccggctt
     7681 tccggttgtc tgtaaatatt gtgcccgcga aaaatggtct ggcaggaggc tgcacagtta
     7741 ccgggtatat ataagatggg ttcccgcttc gcggggcttc ggccagtcag tattgtcact
     7801 catatgatat ttttgtgtgt ggacttccat gccgcttttg cggcataacc ggtatctgac
     7861 aatgtctgta agattaattg ttctgcacgc tgttaatttc cggaagtgat ttcttcgtgg
     7921 ccggaagccg gataccttta ctgcttttta tcccagtata aatccggtaa acatctgatg
     7981 cgtaggcaag acgtctctgg ttctggcgtt cggttttcct gaatccggca ttgtatgcac
     8041 caacggcctc ccaggtgacg ccccattttt taaatgctat tgccagataa taagcaccgg
     8101 tataaatgtt catgcaggga tctgttgtca gatgttctgg cttaattccg tagcgggcca
     8161 gttcgttaaa atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat
     8221 taataccgat ggcattaacc cggtaacggg attcttgcca tgatattgct ctcagtaaat
     8281 caggatctat tttgtaatcc cggcctgcaa gatcaaagca atcagtggcc tggcagatct
     8341 catttataaa catcaggcag atggctaaca tccatttttt catttttcca cctctggtga
     8401 ctttatccgt aaataattta acccactcca caaaaaggct caacaggttg gtggttctca
     8461 ccaccaaaag caccacaccc cacgcaaaaa caagtttttg
//
LOCUS       DAMZII010000180         8468 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZII010000180 DAMZII010000000
VERSION     DAMZII010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.180"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            485..745
                     /locus_tag="POA77_004333"
     CDS             485..745
                     /locus_tag="POA77_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6367670.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            742..1290
                     /locus_tag="POA77_004334"
     CDS             742..1290
                     /locus_tag="POA77_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDI6367671.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1290..1514
                     /locus_tag="POA77_004335"
     CDS             1290..1514
                     /locus_tag="POA77_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367672.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1511..1834
                     /locus_tag="POA77_004336"
     CDS             1511..1834
                     /locus_tag="POA77_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HDI6367673.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1849..4182
                     /locus_tag="POA77_004337"
     CDS             1849..4182
                     /locus_tag="POA77_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6367674.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGVMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGVWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
     gene            complement(4779..7232)
                     /locus_tag="POA77_004338"
                     /pseudo
     CDS             complement(4779..7232)
                     /locus_tag="POA77_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
     gene            7297..8459
                     /locus_tag="POA77_004339"
     CDS             join(7297..7558,7558..8459)
                     /locus_tag="POA77_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDI6367675.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT         2086 a         1957 c         2089 g         2336 t
ORIGIN      
        1 cctccctgca ctcagttgca tttcaggaaa atattcataa aaatgaattt gtctgatgtt
       61 cattaaacac atccggatac atgcggaagt aagatggtaa tttaatttct ttaatcatca
      121 gatttattga tacatatggt gagctgttta tttttggccg ttttggctct tttattaatc
      181 aggtgttgtc ttagtgctta tgatgtaacg ccctgaatga tatgggctgg ttcagatttg
      241 agctgtgatc ctgcctagtg taatagactt ttttattatt gatgctcctt attatattcc
      301 ataaaggaat tgtttgtttt tgttcgtttc atacggtttt tagctgtttt attttttgag
      361 aaatcaacat ttcacacaaa ataaataaag catgcgcgag ccgcttatat aaacataata
      421 aggagctacc tgaatccgca tggaattgcc cggactgttg taaacatatt agagaggtag
      481 ccagatgaat agtatttccg tttccacccc ggcaccagct gctccggtgc tcccggttcc
      541 ggctcagcag gagcgcttca tacgattacc tgaagtgatg cacctttgcg ggctatcccg
      601 ttcaaccatc tacgacctta tcagccgcga tgccttcccg cagcagatat ccctgggtgg
      661 aaaaaatgtg gcctgggtac agtcggaggt cagtgcatgg atggcggcgc gaattgctgc
      721 ccgcgagcaa ggaggccatg cgtgaaactt gctttgcagt ctcatcttct tttttctggc
      781 ttgcgtcctg atatcttttc caggtatagt tttcccgctg ccgcaaaatc ggctgccggg
      841 cgtaggaacc cgagttactt attggcgaca ccagacgcgc cgtgcgtctt tttttacgtc
      901 gttgctcagg cacacccatt tttcgggctg cggtgcttat accttagccc ctttcagata
      961 atggtggtcc gggcggggca gccttcgggc tggccggttt ccaataaggc cggtattcct
     1021 acccccgttc gggtcaccac ccatgagcgt aggaactccg gtggtggcat ttaccgctac
     1081 ttattggagg ttgcccttat ggctacgatc ctcgccccgt cacatcctca atttatattt
     1141 gtttttgccg ccgttcgtcg tgcagaacgt aaaccacgta tcagtatgct gcgtatcatc
     1201 gccagcaatg agcatcatgc acgtctttca ctggtccatg agtatgtgct ttgtcttgct
     1261 gctcgcctgc ctgtccggga ggtgatctaa tgaatcacgc cgcaatctct tatgacgata
     1321 ttctccgcct gaagcatctt cgtaacgtgg gagagtttgt cactggaatg gctgttcttc
     1381 aggattgcta cgaaaaacct gccagtgctc aatgtgaaca actggtttcc ctgatttatc
     1441 tgatgacaga gcagcttgat ggcgtggtac agcgttgcca ggatgacctc atgaacatgg
     1501 aggtggtgca atgaaatcac gcactctttc cctttcactt cgggtagcgt tgtatcgccg
     1561 cgcggtagcc tgcgcctggt tgagtgcctg ctatcagcaa aaccgccatc tgcagctcac
     1621 tctggatgaa attgaaaccg ctattgcccg tgagcttgaa ggattttatc tgcgacagca
     1681 cgggcaaaca aaaggcatgg aaatcgcctg cgccctgctt tcagacctga tggaatacgg
     1741 ccctctaatg tcctgtcccg cactctccca gctcggaata gccgtaatgg atgagttatg
     1801 cgtccgtcac atcaaaaagc cggttttaca ctgagggaga actgcgtcat gtcaggaatg
     1861 aaagtcagtc aggccgtgaa ggctgcccgc ggtcactggg ctcagattct gccagcgctg
     1921 ggcgtgaata tattgaaaaa tcgccatcag ccttgcccgg tctgtggcgg taaagatcgt
     1981 tttcgcttcg acgatcagga aggacgcgga acatggttct gcaatcagtg cggagccggg
     2041 gatggtttag cgcttgtcac cagggcactg aatgtggata tcagtgaagc agctgacagg
     2101 atacatggac tgacacatga tctgtctgta gttaattccg aggtcaggac gttaaccgcc
     2161 gataccgata gcgggaaaga tgcagcggca gcacttgccg cgcgactgct gcaagcctcg
     2221 cgtgaatctg ccggaaacac ttatcttaca cataaaggtt tcccggagca tatttgccat
     2281 gagctgactt cagctcataa ggccggtggt gtgatgttcc gccccggtga tttgatcgtc
     2341 ccactctata acgctgacgg ggagctggtg aatattcagc tcatcagtgg caatggtagc
     2401 aagtgttttc ttaaaggagg tcaggttaag gaggcctatc atctgataga agggggcggg
     2461 agttctgtaa gaaggatttg gattgcggaa ggttacgcca cggcgcttac cattcattat
     2521 ttgacaggag aagccgtcat ggtggcattt tcgtcggtca actttctttc tctgagcagc
     2581 gttgcccata ataagtatcc gggatatcag ttaattattg cagcggaccg cgatctgaat
     2641 ggttcaggcc agaacagggc tgaaactgct gcaaaagcga gtcagtgtga cattgtgctg
     2701 ccaccggttt ttggtgactg gaatgatgcg cttacccaat acggagagga atccacccgg
     2761 aaggcaattc ttgaggcttt gaagccacat aacgccagtc ctttcgacac aatgagtgaa
     2821 gcagaattca cagcgatgag tgtcagcgaa aaagctcaga gggttcggga gcattacagg
     2881 gatgcactgg cggttgatcc gaacgggcag cttttatccc gctacgagtc tggagtgtgg
     2941 aaagttattt ctcagtcaga ttttgcccga gatgtcgcag cccttttcca gcgcctcggg
     3001 gcaccgtttt cctcaggaaa aattgcctca ctggtggaaa cattaaagtt aattgttccg
     3061 cagcagcaaa atccgtcacg tcatctgatt ggttttcgta atggcgttct cgatacgcga
     3121 acagggcttt ttagtccgca ctgtaaagag aactggctgc gtaccctgtg tgaagtcgat
     3181 ttcacgccac cggtaaaggg ggaaacgctt gaaactcatg ccccggcatt ctggcgttgg
     3241 ctggaccggg ccgccggaca caagccagca aaacgcgaca ttattcttgc agcgttattt
     3301 atggtgctgg cgaaccgcta tgactggcag ctctttctgg aagtgactgg ccctggtgga
     3361 agtggcaaaa gtattctggc tgaaattgcg accatgcttg ctggagagga taacgccacc
     3421 tctgcgacca ttgaaacgct ggagtccccg cgagaacgtg cggctctgat aggtttttct
     3481 cttattcgcc tgcctgatca ggagaagtgg agcggcgacg gcgccgggct taaggctata
     3541 actggtggcg atgcagtatc tgtggacccc aaatatcaga atgcttattc aacacatatt
     3601 ccggcggtta tcttggccgt aaataataat cctatgcgct tcactgatcg cagtggtggt
     3661 gtgtcccgca ggagagttat tctgcatttt cctgagcaga tagcaccgga agaacgcgat
     3721 ccgcagttga aagataaaat tgcacgagaa ctggctgtga ttgttcgtca gctcatgcag
     3781 cgtttcagcg atccgatgag cgccagaaca ttgcttcagt cgcagcagaa ttctgatgag
     3841 gctctcacca tcaagcgtga tgctgatcca gcatttgatt tttgtggcta ccttgaggca
     3901 ttacctgaca ccaacggtat gtttatgggg aacgccaata ttgtcccacg ccagccacgt
     3961 acataccttt accatgccta cctcgtatac atggaggcta atggctataa aaacaccctg
     4021 agtcttacga tgtttggcaa agggttgcca gtcatgctga aggagtacgg gctacattat
     4081 gagaaacggc gaactaatca gggaatgcaa actaacctca ctttgaaaga ggagagtaat
     4141 gcagactggc tgcccaagtg tgatgagcca atattaaaat aacgattaac taacccggct
     4201 gatgccgggc ttcttattgg aaacgcacaa atgtagagat agctgttcac tcttcacctc
     4261 gctgttaaca cataaacaat tgatatataa gttaaaaatg acagggtgaa tagtgtgcat
     4321 ggttttgcga aaaaaaagta ttttttgtag agtcaagtcc cggtttggat ccgtttaacg
     4381 gaacctaact agattgacta tttctctgat gatttttgga taaataatca acaaaaatca
     4441 aaatgtgaac agagaatggt gggggcacaa aaaggggcat aattgaatta attataatta
     4501 aatttcaatt tttacaatag gttaaatgtg tgtgcgagtc cggccttcgc accacaccgt
     4561 atgcaaatcg agtcgctcaa gagcggcttt ttttgtgtct gaaatccgtt agttacctat
     4621 ctttctcgct attttaactc tctcagggct aaccaattcc aacccatcgc aaacgaaaaa
     4681 tgttggtaca ggcgttggta ttttcggttc gatattaatt tgtattagct cagtcctgat
     4741 ctgacagcta tctgccagat caggttcgga ttaatacatt atatctgccc gcctacacct
     4801 ttaaacttct caataaacgc gacgatcttc tggaaaaccg tctgtttttt cgtcttatat
     4861 tgcggattta acggactgag ttttggcaat gtctcgttta attccgtgcc attttcggtg
     4921 gcgtattcgc gttttaaaga cgtgcgaata tagcgtttcg ccgcctcttc attgagattt
     4981 tcttctttga tcaaggcttc tgcttcacgt tgctgttcgc gttgagcaaa ggtaaaaaac
     5041 gcgtcaatga tgctggcttt atccggcaaa tcatccaggt tcgtttgctg aataaaatcg
     5101 accaccagac cttctttggc acggttccca aggcttgaac gaattaagcg tttgacctct
     5161 tcggtcattt cgcccttacc tttgttttgt ctgttgtgtt cgaaaatcag tccaaggata
     5221 taatccaggt ttatttcctg agacttcagc aaatcgattt caaaaactac gtcatcccag
     5281 tcagtggttg atttttcttt tttgtcagct tctttctcac ggcgctgcca gtcacgaata
     5341 tcgttatagg cagaacgata atcctgaatc ttgcgttcag cagggagacg aatcgtttgt
     5401 aattcagcga acttttcatc atccacataa tgttcttctt tgaatttttc taccgcaaca
     5461 ggatcgctaa gatcgatttg ttgcagggct ttcagcgtgg caaattcatc atagttttgc
     5521 aggatgttct cggcacgcag atattcacca aacagtttaa cgaagtcttt cttctctttt
     5581 tcactttcaa tattggcagg gtcagggaac tgttgttcca gttctgaaac tactgccata
     5641 aagccgcgtt tagcttcacc agtggcagca tcagtaaagc cttccatata ctctgcataa
     5701 ctcttttcta acaccacatt tttggtattt ttatcaccaa acagcgttat ggcatcaatg
     5761 gttgagcgtt ccagatcccg gaaagtgacg atgttaccga aggttttagt ggcgttataa
     5821 atgcggttgg tgcgggaaaa tgcctgcatc aggccgtgaa aacgcaagtt tttatcgacg
     5881 aatagcgtgt tcaatgttgg agcgtcgaag ccggttaaaa acatcccaac gacaattaac
     5941 agatcgatat cctgattttt aacccgttgg gctaaatcac gatagtagtt ctgaaaaccg
     6001 ttaccgtcgg tgctgaagtt agttttaaaa tagctgttat actcacgaat tgcagcgtca
     6061 agaaactctt tagcactgct gtccattgcg ctggtatcaa aagtttcatc ggaaatttca
     6121 ccaatggcat tttgttcttc attagcggca aaggagaaga ttgtcgcaac acgcagcggt
     6181 ttataggtag ccgatttatt agctgcttcc tcttgtaacc gtttaaacgt cgcataatag
     6241 gcttttgcag catccacgct gcttactgcc agcatggcat taaaaccttt ggaaccaggg
     6301 aaagtacggt gggttttctg gcggaagttg ttcaggatat attgcgtgat ttcctgaata
     6361 cgcatggggt gaagaaatgc ctgctgattt tcagccgcac tcagtttttt ctcgtcagtt
     6421 tctgtctcta aagacttaaa ctgtggccgc acatcgttgt agtccacctt gaatttgagc
     6481 actttttcgt cacgaatcgc atcggtaatc acatacgaat gcaattcacg gccaaatacg
     6541 ctggcggtcg tttctgagcc taaagcgttt tccgggaaaa taggtgtgcc ggtaaaacca
     6601 aactgataat agcgtttgaa tttcttcttc aggtttttct gcgcttctcc aaactggctg
     6661 cggtgacatt catcaaatat aaacaccact tgctgattgt atacaggcag gtcgctttct
     6721 gctttcatca ggttattaag tttctgaata gtggtgacga taattttgtt atcatcctta
     6781 tccagatttc gtttaagacc tgcggtattt tccgagccgt tgacgctgtc tggcgaaaaa
     6841 cgctggtatt ccttcatggt ctggtaatcg aggtctttcc tgtcgaccac aaagaaaact
     6901 ttatcaataa agtccagttc tgttgccaga cgtgcggctt taaagctggt cagggtttta
     6961 ccagaaccgg ttgtgtgcca gataaatcca ccgctttcgg gggtagacca gtttttcgct
     7021 ttataggagc tgttgatttt ccataagata cgctcagtgg cggcaatctg gtacggtcgc
     7081 atcactagta gcgtttggct aatatcgaac acgctgtagt tcactaagac attaagcaga
     7141 gtatgtttct ggaaaaaggt agcggtaaag tctttgaggt ctttaatcag cgtgttgtct
     7201 gatttcgccc aattcatggt gaagtcaaaa ctgatcttac ccagcaatag tggacacgcg
     7261 gctaagtgag taaactctca gtcagaggtg actcacatga caaaaacagt atcaaccagt
     7321 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
     7381 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
     7441 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
     7501 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
     7561 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
     7621 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
     7681 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
     7741 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
     7801 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
     7861 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
     7921 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
     7981 agaagtgggc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
     8041 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
     8101 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
     8161 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
     8221 agcggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
     8281 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     8341 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcggc
     8401 ggcacagttg gtgtggcggc ctcagtccgg aacaatttga aaacaagaac ctcgcttagg
     8461 cctgtgtc
//
LOCUS       DAMZII010000181         8410 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZII010000181 DAMZII010000000
VERSION     DAMZII010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.181"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(60..1652)
                     /locus_tag="POA77_004340"
     CDS             complement(60..1652)
                     /locus_tag="POA77_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDI6367676.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="POA77_004341"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="POA77_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6367677.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="POA77_004342"
     CDS             complement(2850..3968)
                     /locus_tag="POA77_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDI6367678.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="POA77_004343"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="POA77_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDI6367679.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="POA77_004344"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="POA77_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDI6367680.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="POA77_004345"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="POA77_004345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDI6367681.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="POA77_004346"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="POA77_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDI6367682.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="POA77_004347"
     CDS             complement(7218..7283)
                     /locus_tag="POA77_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367683.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="POA77_004348"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="POA77_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDI6367684.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2071 a         2232 c         2019 g         2088 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct
     1321 tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca
     1501 aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgtacgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta
//
LOCUS       DAMZII010000182         8395 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZII010000182 DAMZII010000000
VERSION     DAMZII010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.182"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..278
                     /locus_tag="POA77_004349"
     CDS             <1..278
                     /locus_tag="POA77_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367685.1"
                     /translation="KLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTY
                     IYKSRHEMLPVNLTQETLFSSKSQDKHALFPIFTASWRAHRIMNKGV"
     gene            501..998
                     /gene="ubiC"
                     /locus_tag="POA77_004350"
     CDS             501..998
                     /gene="ubiC"
                     /locus_tag="POA77_004350"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDI6367686.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1011..1883
                     /gene="ubiA"
                     /locus_tag="POA77_004351"
     CDS             1011..1883
                     /gene="ubiA"
                     /locus_tag="POA77_004351"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDI6367687.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2038..4461)
                     /gene="plsB"
                     /locus_tag="POA77_004352"
     CDS             complement(2038..4461)
                     /gene="plsB"
                     /locus_tag="POA77_004352"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDI6367688.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4632..5000
                     /gene="dgkA"
                     /locus_tag="POA77_004353"
     CDS             4632..5000
                     /gene="dgkA"
                     /locus_tag="POA77_004353"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6367689.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5110..5718
                     /gene="lexA"
                     /locus_tag="POA77_004354"
     CDS             5110..5718
                     /gene="lexA"
                     /locus_tag="POA77_004354"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDI6367690.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5791..7116
                     /gene="dinF"
                     /locus_tag="POA77_004355"
     CDS             5791..7116
                     /gene="dinF"
                     /locus_tag="POA77_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDI6367691.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7232..7441
                     /locus_tag="POA77_004356"
     CDS             7232..7441
                     /locus_tag="POA77_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6367692.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7483..7998)
                     /gene="zur"
                     /locus_tag="POA77_004357"
     CDS             complement(7483..7998)
                     /gene="zur"
                     /locus_tag="POA77_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDI6367693.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1922 a         2035 c         2280 g         2158 t
ORIGIN      
        1 ataaattaac acgaaacgat aatatattta tcctgttctc ctctgtcgat gatgggcctg
       61 ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa gataccaatt
      121 ggaattccac gtatatctac aaatccagac atgagatgtt gcctgttaat cttactcagg
      181 aaacactttt cagctccaaa tctcaggata aacatgcgct tttccccatt tttactgcga
      241 gttggcgagc tcatcgtata atgaataagg gtgtttaagt aaaggaaaac atcaccgttc
      301 ctggcatcct gggcggtgat gcccctatgg ttgccctctc cagcgcgggc atcggcaaag
      361 cgtaaggttc aacagcgttt taccacttca tgcgattgtt gcgtttttgt tgcgtattag
      421 atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat gcctttacgt
      481 tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct
      541 attttaaaga gatccctgcc ctggaaccgc aactgctcga ctggctgttg ctggaggatt
      601 ccatgacaaa acgttttgaa cagcagggaa aaacggtaag cgtgacgatg atccgcgaag
      661 ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa gagtctcgtt
      721 actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg
      781 tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt acaaaaattg ggtaaaacgc
      841 cgttaggacg ctatctgttc acatcatcga cattaacccg ggactttatt gagataggcc
      901 gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgc
      961 taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga
     1021 gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat aagccaattg
     1081 gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc
     1141 ctcagctctg gatcctggcg gtatttgtcg cgggtgtctg gctgatgcgc gctgctggat
     1201 gtgtggtgaa tgattatgct gaccgaaagt ttgatggtca tgttaagcgc acggcgaacc
     1261 gaccacttcc cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt gtagtgctgg
     1321 tactgatttc gtttttactg gtactgacgc tgaatacgat gaccattctg ttgtcgattg
     1381 ccgctctggc gttggcgtgg gtgtacccgt ttatgaagcg gtatacccat ctcccgcaag
     1441 tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct gtgagtgagt
     1501 cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg gtggcttacg
     1561 acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt aaatccacgg
     1621 caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt ggcgtactgg
     1681 cactgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac tggtcaattg
     1741 tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag
     1801 cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt ttaggactgg
     1861 caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc ggctttcttc
     1921 tgggttgcct tatgcgcggc gcttctcagg cctacacaac acatcgcaat ttattgaatt
     1981 tgcagattat ggaaggccgg ataaggcgtt ttcgccgcat ccggcaattc tctctgatta
     2041 cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc
     2101 cagcaactga taaaccttca tcgtttctgc cggttcggcg tcgccgctat cgctgatata
     2161 cccttcatca cgcagcgtca gcaccagaga actgaacacc gccttgtcga agaactccgg
     2221 cgcgttgatg ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct ctttctccag
     2281 cgtaccgcgg ttgatcgacg ggttggcact caacaaccag aaggtgatgg cataacgttg
     2341 cagcgtttcg cgcgcgcctg cggccagcag ctgtagggtg cgagaatgcg ccgggttgat
     2401 atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat ttgccagcgc
     2461 atcaataacg tccggcaact cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat
     2521 tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt
     2581 gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt tgcgatagta
     2641 ggtcatcagc accgcttgct cgcgtggcag aatgatgatg tcgccgatag tgtctttctc
     2701 gacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg
     2761 aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc agttgagttg
     2821 ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgat gccagaagcg cagtacaaca
     2881 caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat cggcagcaat
     2941 attattgacc gtcggcgtta accaggccgg acgcaccgct tcgatgggat cgatagattc
     3001 acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt caccgaagtt
     3061 gacgtaaccc tgaccgagat tacgcagctt gcttaaaccg cgcagcatct gcggcaggct
     3121 ctctttctct ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg
     3181 ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc
     3241 ctgaatggtc atcgacagcg taccggtttt cggatccagc aaacgccccg tgcgggaacg
     3301 accgccttcc acgaagtact cgacggaata accacggctg aacagttcgc cgagatactc
     3361 acggaaaacg gtggaataaa gtttattgcc tttaaaagta cggcgaataa agaacgcccc
     3421 cagacggcgg aaaatcggcc cggccggcca gaagttcagg ttgatcccgg cggcgatatg
     3481 cggcggcacc agcccctggt ggtacagcac gtaagaaagt agcaggtagt ccatgtgact
     3541 gcggtggcaa ggcacataca ccagctcatg accgtcgtgg gccagctggc gaacgcgctc
     3601 ggcgttatgg acgttgatgc cctgataaag tcggttccag gtgaagccca gaatgcggtc
     3661 agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca gcgcaatcgc
     3721 gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt ctaccgcttt
     3781 ggcaatggcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag gcagacgtgg
     3841 gcccacagca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg
     3901 tgcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaaa ctgacggcga
     3961 gaaacgcaca aaactgtcgc gaccgagcca cagtacggcg aaaaatttct gtacgccgtt
     4021 aagcatacgc agcggcgggt ttacttcgcc tttttcacgc cccggcgcgc gaccaaacat
     4081 caccgatact ggcaccatct gcacatccag atttgggttg ctgcggtgca aatcaagata
     4141 gtcgtggaac agcttaatag actcttcttt cggcgtgtaa taggtgaaca cacgcggccc
     4201 gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg gctctaacgg
     4261 gtcaggcaag tcatgagcca gacactgggc gcgcaacgtc agcaaatctg ctttcgagtt
     4321 gtacggtaaa acgtacataa ttggacgaga ggtatccagc cccagttccg gggcaggatc
     4381 tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta atttgtagta
     4441 aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg caaatgcgcg
     4501 gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt cgcgcggcgt
     4561 tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca taatgtaaaa
     4621 aggttctttc aatggccaat aataccactg gattcacccg aattatcaaa gctgctggct
     4681 attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt caggaaggcg
     4741 tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca attacccgcg
     4801 tgctgcttat cagctccgtg atgctggtga tgattgtgga aattctcaat agcgccatcg
     4861 aagcagtggt tgaccgaatt ggctctgaat accatgagct ttccggacgc gctaaagata
     4921 tgggatccgc cgcggtgctg attgccatta tcgtcgccgt gattacctgg tgcattctgt
     4981 tatggtcgca ttttggataa cccttccaga tttcgataaa tctctggttt attgtgcagt
     5041 ttatggttcc aaaatcgcct tttgctgtat atactcacag cataactgta tatacaccca
     5101 gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat
     5161 cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc
     5221 cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa
     5281 attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg
     5341 ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc aacagcatat tgaaggtcat
     5401 tatcaggtcg atccttcctt gttcaagccg aatgctgatt tcctgctgcg cgtcagcggg
     5461 atgtcgatga aagatatcgg cattatggat ggcgacttgc tggcagtgca taaaactcag
     5521 gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac cgttaagcgc
     5581 ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt taaaccaatt
     5641 gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg ggttattcgc
     5701 aacggcgact ggctgtaaca tatctctgag accgcgatgc cgccaggcgt cgcggtttgt
     5761 ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc tgataaagca
     5821 ctctggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt gctgggactg
     5881 gtcgatacgg cggtaattgg tcatcttgat agcccggttt atttgggcgg cgtggcggtt
     5941 ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat gagcaccacc
     6001 gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg tgcgctggtg
     6061 caaccgttgc tgttggcgtt gggggctggg gcgttaattg cgctgctgcg tacaccgatt
     6121 atcgatctgg cgctgcatat tgttggcggt agtgaagcgg tcctggaaca ggcgcggcgc
     6181 tttcttgaaa tccgctggtt aagcgctccg gcgtcgctgg cgaatctggt attactcggt
     6241 tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc
     6301 ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt gcagggcgcg
     6361 gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct aatggtgcgt
     6421 aaaatcctca aactacgcgg aatttccggc gaaatgctga aaactgcctg gcgaggaaac
     6481 ttccgtcgct tgctggcgct taaccgcgat atcatgctgc gttcgctgtt gttgcaactc
     6541 tgtttcggcg cgatcaccgt acttggtgcg cgactgggga gtgacattat cgctgttaac
     6601 gcggttctga tgacgctact cacctttacc gcctatgcgc ttgatggttt tgcctacgcg
     6661 gttgaagcgc actccggtca ggcatacggt gcgcgcgacg gtagccagtt gctggatgta
     6721 tggcgggcag cgtgccgcca gtcggggatt gtagcgttac tgttttcggt ggtttatttg
     6781 ctggcagggg aacacatcat tgcgttgctg acgtcgttaa cccagattca gcagctggct
     6841 gaccgctatc ttatctggca ggtgattttg ccgttggttg gcgtctggtg ttatctgctg
     6901 gacggcatgt ttataggcgc aacgcgtgcc gccgaaatgc gtaacagtat ggcggtggcc
     6961 gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tgggtaatca tggtttgtgg
     7021 ctggcattaa ccgtctttct ggcgttgcgc gggctttctc tggcggctat ctggcggcgt
     7081 cactggcgca acggtacctg gtttgccgca acgtgacggt taaaaattct gaataaatta
     7141 tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt gaacgtactc
     7201 tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg gcggtaactg
     7261 gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg atgatatgac
     7321 gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt atggttatca
     7381 gaaagatcag gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat atcgctggta
     7441 attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg gtttcttttt
     7501 cacctgcaca gagtgatcat gctggcactg ttcaggatga cgacacgctt ccacttccac
     7561 gcatgccgca cagagcccat gtgcttcaat cacattatgc cgcagggcaa accccatttt
     7621 tgccgccagc gtatgcataa tgtcttccac gccttctgca cactcttctt tcaccgcgcc
     7681 gcagcgatcg caaataaaca tggctgacgt atgcgtgggc tggtcgaaca gatgacagag
     7741 cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca gaaaatccag
     7801 cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagca aatcaagcag
     7861 atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt ccaggcgctg
     7921 tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta ataactcctg
     7981 cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc ttactttacc
     8041 atgttccagg aaaaacaccg agaaccctta ttgttgccgt aatgttgatt ttctgttttg
     8101 tacgtaaggt gttgtgttgc ttggtcgtac cattatcaac acgataataa ttaataattc
     8161 atttttaaat aaattgcttc aagttaactc gctatttatt taactgggtt tgatcgcata
     8221 cctgtagttc agaaattatg agcgattttt gatagtgacc agatggcaaa agaattataa
     8281 ggaggacacg gtacgacaaa aaaggtagat gaatcatgtt gaagataata cccggtgcaa
     8341 ctggatactt taataaaaca ctcaattcaa atcaattcga taatgaagat gcaat
//
LOCUS       DAMZII010000183         8380 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMZII010000183 DAMZII010000000
VERSION     DAMZII010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.183"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(95..301)
                     /locus_tag="POA77_004358"
     CDS             complement(95..301)
                     /locus_tag="POA77_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDI6367694.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            614..703
                     /gene="kdpF"
                     /locus_tag="POA77_004359"
     CDS             614..703
                     /gene="kdpF"
                     /locus_tag="POA77_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI6367695.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            703..2376
                     /gene="kdpA"
                     /locus_tag="POA77_004360"
     CDS             703..2376
                     /gene="kdpA"
                     /locus_tag="POA77_004360"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDI6367696.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2399..4447
                     /gene="kdpB"
                     /locus_tag="POA77_004361"
     CDS             2399..4447
                     /gene="kdpB"
                     /locus_tag="POA77_004361"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI6367697.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4456..5028
                     /gene="kdpC"
                     /locus_tag="POA77_004362"
     CDS             4456..5028
                     /gene="kdpC"
                     /locus_tag="POA77_004362"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI6367698.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5021..7705
                     /gene="kdpD"
                     /locus_tag="POA77_004363"
     CDS             5021..7705
                     /gene="kdpD"
                     /locus_tag="POA77_004363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDI6367699.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7702..8379
                     /gene="kdpE"
                     /locus_tag="POA77_004364"
     CDS             7702..8379
                     /gene="kdpE"
                     /locus_tag="POA77_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDI6367700.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1739 a         2178 c         2449 g         2014 t
ORIGIN      
        1 taacgatgtc taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc
       61 gctggcggtt ttcaatgtat tcaaaactca gcaatcaata aaaatcacca gttgcctttt
      121 cggcctgatc catccacacc ggtttatcgc tggttttcga ccagacgcga tgcaggtagc
      181 tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg
      241 caaccgcata agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca
      301 tacttttctc ccccttttgt atctacccgg tgaatggcac cggaaaaatg aatttgttta
      361 tctgatgaaa atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt
      421 gcttattgat ggtttattta cattcatcct gtaattaagt tacacaaaag ttaaattaat
      481 actaaacatt agttaaatca tggcttttgc catttttata ctttttttac accccgcccg
      541 cagatttttg cgaaatcttt gcagccagaa ttctaccctt ccggtatcac ttttaggcca
      601 ctggaggtgc actgtgagtg caggcgtgat aaccggcgta ttgctggtgt ttttattact
      661 gggttatctg gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc
      721 ttactgatcg ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg
      781 ctggcgcggc tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc
      841 tttcgtttac ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt
      901 ctcggcctga acatgctggg gctggcggtg ctgtttttta tgttgctcgg tcagcactat
      961 ctgccgctta atccacagca gttgccgggg ctgtcgtggg atctggcgct gaataccgcc
     1021 gtcagctttg tcacgaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat
     1081 ttcagccaga tggctggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg
     1141 gtgatttttg ccctcatccg tgcgtttacc cgccagagca tgagcacact cgggaatgcc
     1201 tgggtcgatc tgctacgcat cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca
     1261 ctgtttttta ttcaacaagg tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc
     1321 gttgaaggag cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag
     1381 atgctcggta ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac
     1441 ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacggcgctg
     1501 tgctttgcct ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg
     1561 atgtcagtga tttttgtcat ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat
     1621 cctcatctgc tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt
     1681 ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg
     1741 gtgattgcga tgcatgattc gtttaccgct ctcggtggca tggtgccgat gtggctgatg
     1801 caaattggtg aagtggtgtt cggcggtgtc ggttctggtc tttacggcat gatgctgttt
     1861 gtcctgctgg cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt
     1921 aaaaaaatcg acgtacgcga gatgaaactg actgcactgg cgattctggt taccccgacg
     1981 ctggtgctga tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc
     2041 aaccctggcc cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac
     2101 aacggcagcg cctttgccgg attaagcgcc aactccccgt tctggaactg tttactggcg
     2161 ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg
     2221 ctggtgagta aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg
     2281 tttgttggcc tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc
     2341 ctggcgcttg gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat
     2401 gagtcgtaaa caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc
     2461 ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat
     2521 cggcagtctg ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa
     2581 tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt
     2641 cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa
     2701 aaaaactgcc tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt
     2761 tcctgccgat caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc
     2821 ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga
     2881 atcggcaccg gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg
     2941 tattctttct gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga
     3001 tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct
     3061 gactatcctg ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc
     3121 gttttccgcg tggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg
     3181 tctgatccca accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagccg
     3241 gatgctaggc gcaaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt
     3301 tgacgttctg ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga
     3361 gtttatcccc gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc
     3421 gctggctgat gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc agcgttttaa
     3481 cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag
     3541 ccggatgagc gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat
     3601 tcgtcgccat gttgaggcta acggtggtca cttccctacc gatgttgatc aaaaagtcga
     3661 tcaggttgcg cgtcagggag ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg
     3721 cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cgcaactgcg
     3781 ccaaatgggc attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat
     3841 tgctgcggaa gcgggcgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc
     3901 attgattcgt cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa
     3961 cgatgctccg gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gtactcaggc
     4021 ggcgaaagaa gcgggcaata tggtcgatct cgactctaac ccgaccaagt tgatcgaggt
     4081 ggtacatatt ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc
     4141 caacgatgtg gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca
     4201 gttaaatgcg ctgaacatca tgcgccttca ttcgcccgac tccgccattc tcagtgcggt
     4261 cattttcaac gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta
     4321 taaaccgctt accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg
     4381 gctgctggtg ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct
     4441 ggtgtgaggt ttaccatgcg tggcttacgt ccggcattat cgacatttat ctttctgtta
     4501 ttgattactg gcggcgttta cccgctgctg accaccgtgc tggggcaatg gtggtttccc
     4561 tggcaggcca acggttcgtt gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc
     4621 gggcagaatt ttaccggcaa cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg
     4681 ccctataatc cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat
     4741 aaacaaatag ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagtacgaac
     4801 gttccggttg aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa
     4861 gcggcggtct ggcaaatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc
     4921 acgcaactga tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc
     4981 gtcaacattg ttgaactcaa tctggcgctg gataaacttg atgaataacg aacccttacg
     5041 tcccgacccc gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt
     5101 tttcttcggt gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg
     5161 actgcgggcg caagggctgg atattgtggt tggcgtggta gaaacccatg ggcggaaaga
     5221 taccgccgcc atgctggaag ggctggctgt tctgccgcca aaacgcctgg cgtaccgtgg
     5281 gcggcatatc agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt
     5341 aatggacgaa ctggcgcaca gtaatgcgcc tggttctcgt catcccaaac gctggcagga
     5401 tattgaagag ctgctggaag caggcattga tgtttttact accgtcaacg ttcaacatct
     5461 ggaaagcctg aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc
     5521 cgatcctttt ttcgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct
     5581 gcgccagcgg ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga
     5641 acattttttc cgcaaaggca atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc
     5701 cgatcgcgta gatgagcaaa tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg
     5761 gcacacgcgc gacgcgatcc ttttatgcat cgggcataac accggcagtg agaaactggt
     5821 ccgcgcggcg gcgcggttgg cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga
     5881 aacccctgcc ctgcatcgct taccggaaaa aaaacgtcgg gcaattctca gcgccttacg
     5941 tctggcgcag gagctgggcg cggagacggc tacactttct gatccagcgg aagaaaaagc
     6001 agtagtgcgt tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc
     6061 gcgccgctgg tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct
     6121 cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga
     6181 tagccgctct tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc
     6241 gttatgcgcc gttatcactt taattgccat gcagtggctg atggcgtttg atgccgccaa
     6301 cctggtgatg ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc
     6361 ttcggtggtt gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg
     6421 cggcacgctc gctgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt
     6481 cgggctggtg atcgggaacc ttactgcggg tgtgcgttat caggcgcggg tagcccgtta
     6541 ccgcgagcaa cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag
     6601 tccgcaggac atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag
     6661 tcaggtgttg ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat
     6721 gacgccatgg gacgatgcca tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc
     6781 gggcaccgac acgttacccg gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa
     6841 aacctacgga ctggtggtgg tggaaccggg gaatctgcgt cagttgatga tcccggagca
     6901 gcagcgcctg ctagagacgt ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct
     6961 gaccgccagt gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgccct
     7021 gctggcggcg ctttcgcatg atttacgcac gccgcttacg gtgctgtttg gtcaggcaga
     7081 aatcttaacg ctcgatctgg caagcgaagg atcgccccac gcccgccagg ccagcgagat
     7141 tcgtcagcat gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat
     7201 tcagtccggc ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tagtcggcag
     7261 cgcgctgcaa atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc
     7321 gctgacctta atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga
     7381 gaacgcggtg aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg
     7441 cgaaaatcta caactggatg tctgggataa cggccccggt cttccgccag gccaggagca
     7501 gacgatattt gataagtttg ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct
     7561 tggactggca atttgtcggg cgatagtgga tgtacacggg ggcactatta ccgcgttcaa
     7621 ccgaccggaa ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact
     7681 tgaagacttt catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt
     7741 cgtcgctttc tgcgcacggc gctggagggc gacgggatgc gcgtctatga ggccgaaacg
     7801 ctgcaacgcg gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc
     7861 ggcctgcccg atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg
     7921 ccggtgattg tgctttccgc acgcagcgaa gagagcgaca agatcgccgc gctggatgcc
     7981 ggagcggatg attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc
     8041 gtcgctttac gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat
     8101 gttaccgtcg atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcaca
     8161 ccgattgagt tccgcctgct ggcggtgctg ctgaataatg ccgggaaagt gctcacccag
     8221 cgccagttac tcaatcaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt
     8281 atttatatgg gacatctgcg acaaaaactg gaacaggatc ccgctcgccc ccgccatttc
     8341 attactgaaa ccggtattgg gtatcggttt atgccatgaa
//
LOCUS       DAMZII010000184         8085 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMZII010000184 DAMZII010000000
VERSION     DAMZII010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.184"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            103..1236
                     /gene="ompN"
                     /locus_tag="POA77_004365"
     CDS             103..1236
                     /gene="ompN"
                     /locus_tag="POA77_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDI6367701.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1377..1811
                     /gene="uspF"
                     /locus_tag="POA77_004366"
     CDS             1377..1811
                     /gene="uspF"
                     /locus_tag="POA77_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDI6367702.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1987..2922
                     /gene="ttcA"
                     /locus_tag="POA77_004367"
     CDS             1987..2922
                     /gene="ttcA"
                     /locus_tag="POA77_004367"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDI6367703.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3051..4424)
                     /gene="dbpA"
                     /locus_tag="POA77_004368"
     CDS             complement(3051..4424)
                     /gene="dbpA"
                     /locus_tag="POA77_004368"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDI6367704.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4902..5885)
                     /gene="zntB"
                     /locus_tag="POA77_004369"
     CDS             complement(4902..5885)
                     /gene="zntB"
                     /locus_tag="POA77_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDI6367705.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6140..7372
                     /gene="dgcM"
                     /locus_tag="POA77_004370"
     CDS             6140..7372
                     /gene="dgcM"
                     /locus_tag="POA77_004370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDI6367706.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7393..7956)
                     /gene="smrA"
                     /locus_tag="POA77_004371"
     CDS             complement(7393..7956)
                     /gene="smrA"
                     /locus_tag="POA77_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDI6367707.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2136 a         2032 c         1908 g         2009 t
ORIGIN      
        1 taacaaatga tatttatcgg ctaactgaac ttctccttta ggatgttttc actccccgaa
       61 atggggataa aaggcaaata aaataactaa aggatttatt caatgaaaag caaagtactg
      121 gcacttttaa ttcctgccct gctcgccgca ggtgctgcac atgcagccga agtttataat
      181 aaagacggca acaaattaga tctgtatggc aaagttgatg gcctgcatta tttttctgat
      241 aattcagcga aagatggcga ccagagctat gcgcgtctgg gttttaaagg cgaaacccaa
      301 attaacgatc aactcactgg ctacggtcaa tgggaataca atattcaggc aaacaacact
      361 gaatcttcaa aaaaccagtc atggacccgt ctggcatttg ccgggctgaa atttgcagat
      421 tacggttctt tcgattacgg acgtaattat ggcgtaatgt acgacatcga aggctggacc
      481 gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt tatgactggt
      541 cgagccaatg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt aaatggtctg
      601 aacttcgcgg tgcagtatca aggtaacaac gaaggagcca gtaatggtca ggaaggcacc
      661 aacaacggac gtgatgttcg ccatgaaaac ggtgacggct ggggtctttc cacaacatat
      721 gatttaggca tgggctttag cgctggtgcg gcatacacct cttctgaccg caccaatgac
      781 caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc tgggctaaaa
      841 tacgatgcta acaatattta cctggcaacc atgtattcag aaacgcgtaa tatgaccccg
      901 tttggcgaca gcgattatgc tgttgcaaac aaaacccaga actttgaagt cactgcacag
      961 taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa aggccgtgac
     1021 ctgcacgctg ccggtggtgc agacaacccg gcaggtgttg atgataaaga tctggttaaa
     1081 tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccactta tgttgactat
     1141 aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaatggcat ctctaccgat
     1201 gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg
     1261 ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac tccgtcatta
     1321 cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga ggttttatga
     1381 acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa cgcgtgatta
     1441 gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg acggtaatac
     1501 cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta ccggcaatgg
     1561 atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa tttaaactgc
     1621 caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc attctggaaa
     1681 tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg gatatcacca
     1741 cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg
     1801 ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg
     1861 tgctgacatt ttcccctcta atccgtacca tacacgccac tgtttttata tcagatttct
     1921 tatgctgggc attccggcat gacgcatact cttctgatgc catataacga attgagtcgc
     1981 ttttaaatgt cgcaaaatca agaaattagt aagaaagaac aatacaacct gaacaaatta
     2041 caaaaacgtc tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat gattgaagaa
     2101 ggcgatcgca tcatggtttg cctctccggg ggtaaagaca gctataccat gctggagatt
     2161 ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt taacctcgat
     2221 caaaagcaac cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt
     2281 gagtacaaga ttgttgaaga gaatacttac ggtatcgtga aagagaagat tccagagggc
     2341 aaaaccactt gctcactgtg ttctcgcctt cgtcgcggta tcctttatcg taccgcaacg
     2401 gaactggggg cgacgaagat cgcgttgggt caccatcgtg acgatatcct gcaaacgttg
     2461 ttcttaaata tgttctacgg cggcaagatg aaaggtatgc ctccgaaact gatgagcgat
     2521 gatggcaaac atatcgttat tcgtccgctg gcctactgcc gcgagaaaga tattcagcga
     2581 tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc acagcctaac
     2641 ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg ataaacgtta tccagggcgt
     2701 atcgagacga tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg cgataccaac
     2761 ctgttcgatt tcaaaggcat tacccacggt tctgaagtgg ttaacggggg tgatctggcg
     2821 tttgatcgcg aagagatccc actacaaccg gcgggctggc agccagaaga agatgaaaat
     2881 cagttggatg agttacggct gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg
     2941 cgcaagcgta tcgggctact cttatggagg ccggataaga cgcggccagc gtcgcatccg
     3001 gcaatcccgc ataagatgtt tactcttgca cccggcaatt caacatttca ttattttaat
     3061 aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct gtaactgttt ccatgcttta
     3121 tgagcaacag cctgacgcac cgcgacatag acatgcgccg gatgcacggc gattttgcca
     3181 atatctgcgc catcaagccc gatgtctcct gtcagcgcac ctaatacatc acccgggcgc
     3241 attttggctt ttttcccgcc atcgatacac aacgttgcca tttctgcttc cagcggcaaa
     3301 atggaactat tagctggcgg cgtttgccag ttaagtttta tctgcaacat gtcagaaatg
     3361 atattggccc gctgtgcttc ttccggagca cagaaactga tcgccagacc gctatttcct
     3421 gcacgagctg tacggccgat gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag
     3481 ttcaccacca gctcaagcga tttaatatcc agaccacgcg cagcaacatc agtcgcaacc
     3541 agtacacggg cgctaccgtt agcaaaacgt accagggtct gatcgcgatc gcgttgctcc
     3601 agatcgccgt gtaacgacaa tgcactttgc cctacttcat tcagcgcgtc gcagacagcc
     3661 tggcaatctt ttttggtatt gcaaaacacc acacaagagg atggctgatg caaacttaat
     3721 aaccgttgca acagaggaat tttgccttta ctggatgtct cataaaattg ttgttcaatg
     3781 ggtggcaaag catctgttga gtcaatttca atcgccaaag gatcgcgttg cactcgtccg
     3841 ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg tcgagatgca
     3901 ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc ccatatccag catgcggtcg
     3961 gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt ttgcaagtga
     4021 tccagcaaac gacccggcgt tgccacgata atatgcggcg catgttgcaa cgaatcacgc
     4081 tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg cagaaaacgc
     4141 gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg acacagcact
     4201 aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag gccaaaagcc
     4261 gccgttttgc cgctgccggt tttcgcctgc acgcgaacat cttttccggc aaggatcgcc
     4321 ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt aaggttcgtg
     4381 agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta ttctcgtggt
     4441 catcgacgca aagttagcag gcgcgtatcc tcgcagatct acgctcacga tgcgacaatt
     4501 taatcggttc ttcatcaggt ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg
     4561 gcactggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa taaataagtg
     4621 tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct gataaataac
     4681 aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt
     4741 aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga cgttgcattc
     4801 acctgcaagg gagatattgc cctgaatggg tagagagttt attgacttcg ctcaaacttt
     4861 gcggcgtttt tgtatacaga cagccggaaa aattgctttt gttacaacca tttactacgg
     4921 tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa aattgaaaat
     4981 ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa ccctgtcaga
     5041 aaggtactgg gtaaaaagac cattgccatc aatgacattg tataggtacg acgagctaaa
     5101 ttttcctgca tcacctgagc gatttcatcc gccatcacgc cagtccgtgc tatacaggcg
     5161 tcgatttcgt caagaccgcg cccaaggcga tcggcaatat cctgcatccg acggcgttgg
     5221 tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc acgttgcggt
     5281 gccatatagc gacgcatcac gattaattgt ttgcgcagca gagccaggaa tccacgcggt
     5341 ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg cagctgctcg
     5401 ataaattcgc tggaatgatc ggtcaacgca tcgcacacat ccaccagcca ccctccgcaa
     5461 tcggtcggcc ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc cagcactttg
     5521 cgttgtcggg tcgaaacaat taaccgcccg tccatatata cacgcatggc gaccagttga
     5581 tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt gccttcaccg
     5641 agacggctga ctcggggccg cgtgctctcg cccgccagcg catcacgtac gttattggga
     5701 agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt aaggtggagc
     5761 cagcagggat gcgcttcatc aatcacatct gtattttcca gcggtttaac gccgcctcta
     5821 ccatccagca tccaggcaaa tactgcatcc gggacattaa cgtccgatcc cttaatcgct
     5881 tccacagtgc ctccatcatc aacgcattat tttgtagtct agccttctgg ccctgttacg
     5941 caacatctca tcaccccatt accctgaaat gattaataaa attctgtcta aattgaatac
     6001 aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc gtctatcatt
     6061 atcagaatta tctgatcata tgacgtggct tttttgcgat cggatagcaa caaaaattga
     6121 taaaaataac gggatctcaa tgattacgca caacttcaat accctggact tactcaccag
     6181 tcctgtctgg attgtttcgc cctttgagga acagttaatt tatgccaata gcgcggcgcg
     6241 actgttgatg caagacctca cgtttagtca gctacgaacc ggaccctatt ccgtctcctc
     6301 acaaaaagaa ctgccgaaat acctctccga tctgcaaaac caacacgata ttatcgaaat
     6361 cctcactgtt cagcgtaaag aagaggaaac agcattaagc tgtcggcttg ttttgcgaga
     6421 gctgacagaa acagaaccgg tgattatttt cgaaggtatc gaagcgccgg caacgctggg
     6481 tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa caaggttttt atgcgcgctt
     6541 ttttctgact aactctgcac caatgttgtt gattgacccc tcacgagatg gccaaatcgt
     6601 cgatgctaac ctcgccgcgc tcaatttcta tggttataac catgaaacga tgtgccagaa
     6661 acatacctgg gaaattaata tgctcgggcg tcgcgtcatg cctatcatgc atgaaatttc
     6721 gcatttaccc ggcggccata aacctttgaa ttttgttcat aaactggcgg atggatctac
     6781 tcgtcatgtg cagacctatg ccgggccgat tgaaatttat ggcgacaagc tcatgttatg
     6841 tattgtgcat gatattactg agcaaaaacg gctggaggag cagctggaac atgctgctca
     6901 ccatgacgcg atgaccggat tactgaatcg gcgacagttt tatcacatta ccgagccagg
     6961 ccaaatgcag catctcgcca tcgctcagga ttacagcttg ttgctcatcg acaccgatcg
     7021 ttttaaacac attaacgatc tctatgggca ttctaaaggt gatgaggtgt tatgcgccct
     7081 cgcccgcacc ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt ggggaggcga
     7141 agagtttgtc ttattgctac caagaacccc actggatacc gcgctttcgc tggctgaaac
     7201 tatccgcgta agcgtggcaa aagtgagtat ttcgggctta ccacgcttta ccgtcagcat
     7261 tggtgtggcg catcacgaag gaaatgaaag catcgatgaa ctgtttaaac gcgttgatga
     7321 tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat aagccgcgga
     7381 tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc cagttttctt gcttcgcctg
     7441 cgccgtttta cgtagtgcga cgtaacacgc cccgctgccg ccatgatgcg gtagcgcagt
     7501 gcaatatgcc tgaacatcat caaattcggt cagccagcgc gccacatagc tgcggacaat
     7561 attggcatgc gatttatcat cccgtccttt gccatgaata atcagcacgt tacgcaaacc
     7621 atcagccatg gcttgttgaa tgaaactgaa catcattttg cggcactctt ccaccggctg
     7681 gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca ctgcgcagct tatccagcac
     7741 cccatgttgc aacccttccc gccgaaactc cagcggctga cttagtggga tgatgtcgag
     7801 aaatccggtg gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg gcgcacgttg
     7861 gttacgcgtt ggatgccagt ggacatcggt agcacgtttc agcggctgga catcttccat
     7921 ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtta catcctcccg caattaagag
     7981 cgcgatatga taaccagacc gggtcggtcc aacaacgtat tacccaaaat tccagtaata
     8041 agttccaaat attgccgata ttttaagcaa aatgcttatg catga
//
LOCUS       DAMZII010000185         7992 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZII010000185 DAMZII010000000
VERSION     DAMZII010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.185"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            300..1214
                     /gene="sitA"
                     /locus_tag="POA77_004372"
     CDS             300..1214
                     /gene="sitA"
                     /locus_tag="POA77_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDI6367708.1"
                     /translation="MYSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQEAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1214..2041
                     /gene="sitB"
                     /locus_tag="POA77_004373"
     CDS             1214..2041
                     /gene="sitB"
                     /locus_tag="POA77_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDI6367709.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNASEESIITDDEHPFVAHRPSAVQREAR"
     gene            2038..2895
                     /gene="sitC"
                     /locus_tag="POA77_004374"
     CDS             2038..2895
                     /gene="sitC"
                     /locus_tag="POA77_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDI6367710.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2892..3749
                     /gene="sitD"
                     /locus_tag="POA77_004375"
     CDS             2892..3749
                     /gene="sitD"
                     /locus_tag="POA77_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDI6367711.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFIAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDIHLDHILFGDMLGVTIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(3983..5688)
                     /locus_tag="POA77_004376"
                     /pseudo
     CDS             complement(3983..5688)
                     /locus_tag="POA77_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            5659..5982
                     /locus_tag="POA77_004377"
     CDS             5659..5982
                     /locus_tag="POA77_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6367712.1"
                     /translation="MLWCIIENSLDRHSFLGNGLSTEDTMGKMVIQILAAVAEAEREH
                     ILERTNDGRLVAMVAGVKFGRKPHQKSAMIREFISQSATVKLIIEKTGISRATYFRIK
                     NSPAI"
     gene            6263..7165
                     /locus_tag="POA77_004378"
     CDS             6263..7165
                     /locus_tag="POA77_004378"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDI6367713.1"
                     /translation="MIYVFGHQNPDSDSICSALVTADWLNMLGKFATPFRLGEITPET
                     AFILQQAGVEAPRLLSEDLSGTSVWLVDFTDAEQGPPSLALSNIVGIIDHHRLGTVMT
                     QNPPDVWIRAVGCCATILWQILTFENQLSISSTQATLMLGAILSDTVVLSSPTTTVHD
                     IAAVKALIAISKLDYNPFVKGLLAAKTDIQGQSPHTLLMKDAKRYQINGLSVLLSQIE
                     VAAMSEVDLVLPDLHTALMKECQCGGFDIAAMAITDIFQKNSTLYFSENNALNFASLS
                     LPGVISRKKGILPWLTSAISTSWR"
     gene            7169..7597
                     /locus_tag="POA77_004379"
     CDS             7169..7597
                     /locus_tag="POA77_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6367714.1"
                     /translation="MSMYKHALILLSDESDGQFLLQSVAQSFHEQGTAITIGHLTSDY
                     RELDIASDSLTKDHQSADVIAAKAMLSRLVQSARFPVDVKEIVTIRRLRDVEVCVTEA
                     GVDLVIMGHRNRLFEMISSHSIEFINHLTVDVLIKHITTH"
     gene            7612..7992
                     /locus_tag="POA77_004380"
                     /pseudo
     CDS             7612..7992
                     /locus_tag="POA77_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786687.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
BASE COUNT         1978 a         1931 c         1928 g         2155 t
ORIGIN      
        1 atcccttcat ttagtgactg cccctgggtt tttcatgctc tcctgcgtcc tagagaactt
       61 gccaataacc tacattattt ttgctttgcc attaccaatc gtcctgaaca agttcatttt
      121 aaagccaaag cgctgcccaa cttttatcct caataccaca tcgtcagcag gaaataacct
      181 aatccctgtg ttgggatctt aaaatcgcta atgataatta tttacatttt atagcttctg
      241 ctataaaaca tagccgttgc tatgtttgtt ctaattttgc tcactatagg tactaaatta
      301 tgtactcaat aaaaaaagta accatgctct tggggtgcct cgccctcacc tgctcgatcg
      361 catttcaggc gagtgcaact gaaaaattca aggtcattac aacatttacc atcatcgcag
      421 atatggccaa aaacgtggct ggagatgctg cagaagtctc atccataacc aagcccggtg
      481 cagaaattca tgagtatcag cctacccctg gcgatattaa acgtgcgcag gaggcacaac
      541 tgattctcgc caatggtatg aatctggaat tgtggttcca acgcttttac cagcatctta
      601 atggggttcc agaagtaatt gtctcttcgg gtgtgacgcc agtagggatc actgaaggcc
      661 cctatgaggg caaacctaac ccccatgcct ggatgtcgcc agataatgct ctgatttacg
      721 tcgataatat tcgtgatgcg ttgataaaat acgatccggc aaatgcacaa acctaccaac
      781 gtaatgccga tacttataaa gccaagatta cccaaaccct tgcccccctg cgtaagcaga
      841 ttgctgaact ccctgagaat cagcgatgga tggtcaccag tgaaggggct ttttcttatc
      901 tcgcacgcga tttggggcta aaagagcttt atctgtggcc gattaatgcc gatcaacaag
      961 gcacgccgca gcaggtacgt aaggttgttg atatggtgaa gaaaaatcat atcccggcag
     1021 tctttagcga gagtacgatt tccgataaac cagcgcgtca ggttgcgcgt gaaaccggcg
     1081 cgcactacgg cggtgtactc tatgtcgatt ccctgagcac agaaaacggc ccggtaccta
     1141 cgtatatcga ccttctgaag gttactacca gtacgctggt acagggaatt aaagccggga
     1201 aaagggagaa ataatgatgc aatctgcggg cattgtcgtt aatgatgtta ctgtcacctg
     1261 gcgtaacggg cacacagcac tgcgcgatgc atccttcaca gtaccaagtg gatctattgc
     1321 cgctctggtt ggggtaaacg gttccggaaa atcgacgttg tttaaagcga ttatgggatt
     1381 tgtgcgtctg accagcggaa agatatctgt tctgggtatt cccacgcgac aggcgctaca
     1441 gaaaaacctg gttgcctacg tccctcagtc agaagaggtt gactggtcat ttcctgtact
     1501 agtggaagat gtggtgatga tgggccgcta tgggcacatg ggtatgttgc gtatagccaa
     1561 aaagagggat cgtcagattg tcaccgatgc gctggaacgt gtcgatatgg tggattttcg
     1621 ccatcgacaa atcggcgagc tttccggtgg acaaaagaaa cgcgtctttc tggcgagagc
     1681 gattgcacag cagggtgatg tgatcctgct ggatgaacct tttaccggcg ttgatgtgaa
     1741 aaccgaagcg aaaatcatca gcctgttgag ggagttacgc gcggaaggca agacgatgct
     1801 tgtctcaacg cacaatctcg gctcagtcac gacattttgc gattatacgg tcatggtcaa
     1861 aggcaccgtc ctggcaagcg ggccgacaga caccactttt acagccgaga acctggagct
     1921 ggcttttagc ggcgtactgc gccatgtcac acttaacgcc tcggaagaaa gcattattac
     1981 tgacgatgaa cacccttttg tggcacatcg accgtcagcg gtgcagaggg aagcaagatg
     2041 aacgtgctac tggaaccctt cagctatgaa tatatgctca atgcgatgtg ggtctcggcg
     2101 atggtcggcg gtctctgcgc tttcctgtca tgctatctga tgctaaaagg ctggtcgctg
     2161 attggcgatg cgctgtcgca ctctattgtc ccgggtgttg cgggggcata tatgctgggg
     2221 cttccgtttt ctctgggagc cttcttttcc ggcggactgg cagcgggcag catgctgttt
     2281 cttaaccaac gcactcgctt aaaagaagat gccatcatcg gcctgatctt ctcctccttt
     2341 tttggcctgg ggctgtttat ggtgtcattg aacccgacat ccgtgaacat tcagaccatc
     2401 gtcctaggca atattctggc aatcgatccg gcggatatcc tgcaactcac tatcattggc
     2461 attctctcga tcatcgtgct ttttttcaaa tggaaagatc ttatggtgac ctttttcgac
     2521 gagaatcacg cccgtgctat tggattgcat ccgggcagat taaaaatcct cttcttcacc
     2581 ctgctgtcgg tttcgaccgt tgcggcgctg caaacggttg gtgcctttct ggtgatctgt
     2641 ctggtggtga ctcccggtgc aacagcatgg ttgttaaccg atcgttttcc ccgcctgctc
     2701 ataattgctg taactattgg cagtgtaacc agtttcctcg gcgcgtgggt cagttacttt
     2761 ctggacggcg cgacaggtgg aatcatcgtg gtggcgcaga cactgctgtt tcttttagca
     2821 tttgtatttg cgcccaccca tgggcttttg gcaaatcgtc ggcgtgcgca taaagcgctg
     2881 gaggatcgct catgatggca ttattactgg agccactgca gttcaccttt atgagtcacg
     2941 cactgctgat ttcactggta gtttccatac cctgtgcgct gctgtcggtc tttctggtcc
     3001 tgaaaggttg ggcactgatg ggtgacgcga tgagtcatgc ggtttttcct ggcatcgtgc
     3061 ttgcctggat tctggggtta cctctggcta caggggcatt tatcgccgga gtattctgcg
     3121 cggtggcgac gggatacctg aaagacaaca gccgaatcaa gcaagataca gtgatgggga
     3181 ttgttttttc tggcatgttt gccgcaggtc tgatcttgta tattgcagtc aaaccagaca
     3241 tacatcttga ccatattctt ttcggcgaca tgctcggggt aaccatcggc gatataatcc
     3301 agacgatgat tattgccggg ctggttacac ttgttattag cgtaaaatgg cgggattttt
     3361 tgctgttcag ctttgattat caacaggcgc aggcaagcgg tttgcatacg agatggctgc
     3421 attatgggct gctgtgcatg gtctccctga ccattgtggc gacgctgaaa gcggtaggta
     3481 tcattctctc tatttctctt ctcattgcgc ctggtgccat tgcggtactc ctgacacaac
     3541 gctttcatat tgcgctcctg ctggcgactg gcatatcgat attggtctca atgactggtg
     3601 tctggctgtc cttttttatc gacagcgcgc cagctccgac aattgttgtc ttattcgcgg
     3661 tcatgttcat catgacgttt accgttacca gcatcaacgc acgcacaaag gaaaatgccg
     3721 aaccacggga tttgttatca tccgattagc agaacactcg gccagcaaca tccgcttatt
     3781 ggcgatgcgc ttgaacgaat aatgcacaga cttatgattc tgataagttt tgttgccaga
     3841 gagttgctgc ggactggata gcaagtaatg tcgattctca ggggtagcaa acagcgcagt
     3901 aaaatgaggg aagtagtggc aaaacataga gaaaattggg gggcgagggc cagtgatgta
     3961 aaaaaccacg ctaagagttt aattaattgt ttgtattgaa gtttaatgat cttaactcac
     4021 ccttaagaat atcttccggt aacgaagacg tataatgccc cagcatattt atatggctat
     4081 gtatcagcag cgataatctt gccggatgct actcctcagg agtttctccc gatttgcgaa
     4141 gatgtgacaa cgcctcctgc atgtaaagtg tgtttcacag cacaaccgca ttggtcacca
     4201 gccccagagc acctagttga tcttcctgcc cttaacggta gcgttttcta atctcaccac
     4261 actgtccata gtaaatggct cgtgccacgg catggcgtcc ttctccccgg ttgaactgag
     4321 tgagtatccg acgccggtaa tcctcatcat caatatagtt gagtagatac agagtcttgt
     4381 tgacccggcc cacttccata attacctgag ccagaccaga tggccggcta cttctcaaca
     4441 gtgaacgaat taagttctga tgcatggatg gtcccaagct ttagtgagcc ctccattcgc
     4501 atcatttcat cccattggga ttctattttt gacagctcaa cacatccccg ggccaactca
     4561 ttcagggtac cataatgtgc cgttttatct gcccgccaga acatcgccac acctgcctcg
     4621 gccagacgag gggaaaactg atagccaaat aaccagaaaa ggccaaaaat gatatcgctg
     4681 gatcctgccg tgtctgtcat aatttctacc ggatttagcc cggtttgctg ctctaggagc
     4741 ccttccagta caaagatggg atcacgaagc gtaccgggaa caacaatgcc atggaaacca
     4801 gagtactgat cggggacgaa tttgtaccaa gtgataccgc atcctgaacc aaagtattta
     4861 cgatttggtc cagaattaac cgttttaacc ggtgtgaaaa aacgcatacc atctgcggaa
     4921 gcaacctcat tcccccagta tcccgcgagc ggctgcgtgg actgaaaatc aaccagccgt
     4981 acattagcgc tgaccagcgt ttctgccctg agataatttt gcttaaccca actcagacga
     5041 tggcgagtaa gcgcgggaat attatgcttt atcagaggtt catgtcctat attgcaggct
     5101 tccgctagca gtgttgcgca cagactgatc tggagatcct gcacccgggc acctgattca
     5161 ctgacgtggc tgaattcgcg gataaaaccg gttcgggtat ctatttcaag caacagctca
     5221 gtcagatcaa tggggggatc aatcctctta cccgacgatt caattgtatc agtgccggcg
     5281 gctcgtccag gttatccaga ctgctgattg tcagtgaagg atgcttacct tcattgctta
     5341 tactgaccac cacgttgcag gcaaaacgtg aagccactga tttctatgtg tcattaagcc
     5401 tcgtgaccag ttgctctgcc gctttggttc cgtctgatgg atgacccagc gcccgacata
     5461 ctgggagcat tgtgcgtgct attcagctcc ctgaagcaat ttttgacgcg ggtcacccca
     5521 ccggtcactg ttctccagcc agagatcacg acggcgcaaa gaatcctgaa tacgctcgag
     5581 aaggcacaac aagtatcctg cgcgtagaat cctgccatct ctgtcgtaga ccaggcgttt
     5641 ctaggagccg gagatgatgt gctctggtgc atcattgaga attctcttga tcgccattcg
     5701 tttttgggaa atgggctgag tactgaagat actatgggga aaatggtgat tcagatactc
     5761 gcggctgtcg ctgaagctga acgtgagcac atcctcgaac gcaccaacga tggtcgactg
     5821 gttgctatgg ttgctggtgt taagtttggg cgtaaacctc accaaaaatc agcaatgata
     5881 cgggaattta tcagccagag tgctaccgtt aaactcatca tagagaaaac tggtatttct
     5941 cgtgcaacgt atttcagaat aaaaaactca cccgccattt aacgaattga gaccttaaaa
     6001 attttctgaa ggccctgacg atttagctct ggttgaagaa gcagaaactt ttgttgcgca
     6061 catcacgatt ttcacctatg acctttatca aggtgatggt gttccacctt tcccaaccgc
     6121 cggatttttc agttcggata atgacgcctg atatacattt ttgcttttct ccttaggcac
     6181 gtatgtcagg tactcttatg aaaaaaattc tttatagtca ctcatccatg cattgctcac
     6241 cttatttatt aaaggtgtcg ctatgattta tgtttttggc caccaaaatc ccgatagcga
     6301 cagtatctgc agtgcacttg tcaccgcaga ctggttaaac atgcttggga agtttgcaac
     6361 cccatttcgg ctgggtgaaa ttaccccaga aaccgccttc attttgcagc aagccggggt
     6421 tgaagctcct cgattactta gcgaagactt gagtgggacg tcggtctggc tggtcgattt
     6481 taccgatgcg gagcaagggc ctccttcttt agctctcagt aatattgttg gcatcattga
     6541 ccatcaccga ctgggaactg tgatgacaca aaaccctccg gatgtatgga tacgcgccgt
     6601 cggatgttgt gccactatcc tctggcaaat ccttaccttt gaaaaccaac taagtatttc
     6661 cagcactcag gctaccctga tgttaggagc tattttgagc gatacagttg tactcagctc
     6721 accgacgacg accgttcatg atattgccgc cgtaaaggct ttaatcgcaa tcagcaaact
     6781 cgactataac ccctttgtta aggggttact tgccgctaaa accgatatcc aggggcaaag
     6841 cccccacact ctgcttatga aggatgctaa acgctaccag attaacggcc tctcggtgct
     6901 cctttcacaa attgaagtcg cggccatgtc cgaagtggat ttggtgcttc ccgaccttca
     6961 tacagcactg atgaaagaat gtcagtgtgg cggttttgac attgcagcga tggctatcac
     7021 agatattttt caaaaaaact caacgttata tttctcagaa aataatgcac tcaacttcgc
     7081 gagtttgtca ctgcctgggg ttatcagcag gaaaaaaggc atactgccgt ggttgacgag
     7141 tgctatatca acctcctgga ggtaatcgat gtcaatgtat aagcacgctc tcatattgct
     7201 cagtgatgag tctgacggtc agtttctgct ccaaagtgtt gcgcaaagtt ttcatgagca
     7261 aggaacggcg atcaccattg gacatctcac gtcggattac cgtgaactcg atattgcctc
     7321 cgattcttta acaaaagacc atcagtctgc cgacgttatt gctgcaaaag ccatgttaag
     7381 tcgattagtg cagtcagcca ggttccctgt tgatgtaaaa gaaattgtca caattcgtcg
     7441 attgcgtgac gttgaagtct gcgtcaccga agcgggtgtt gatctggtca ttatgggaca
     7501 tagaaacaga ctgtttgaga tgatctcttc acattccatt gaattcatta accacctgac
     7561 cgttgatgtg ttgattaaac atattacgac tcactaatgc ctgaggttta tatgagcttt
     7621 gttcttgaga aaatcgttgc ctgagagatt ttggattccc gggggaaccc gaccattgaa
     7681 gtcgatgtca tcacccagtg taacactttt gccagagctt ccgtaccttc cggagccagt
     7741 accggttcgc gtgaggccat cgaacgccgc gacgatgata aaactcactt tggaggaaag
     7801 ggggtactca aggcaatcga gagtgttaat acagagatta atgacagcct taaagggcat
     7861 gatgtgcgcg atcaacgtgg cattgatgac attatgattg ccttggacgg aacagaaaac
     7921 aaagcacgcc taggagcaaa tgctattttg ggcgtatcat tagccacagc gcgtctcgca
     7981 gctcagttat ct
//
LOCUS       DAMZII010000186         7911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZII010000186 DAMZII010000000
VERSION     DAMZII010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.186"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..3114
                     /gene="trhN"
                     /locus_tag="POA77_004381"
     CDS             <1..3114
                     /gene="trhN"
                     /locus_tag="POA77_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhN"
                     /protein_id="HDI6367715.1"
                     /translation="WAFYVSLINIALTPKIFAADAIFDQLENNFNLANPNANRNATTS
                     AQDIVEKYKNADSGENLSGKISEKYVGKAESTNLNVGKYGTPNSNESVMSNAVSDGKS
                     IGKAVQLPSMSGGTINSNYTKEGAKLLSRDSSGNIGISNNPNTTAGTKTSTGELFSSE
                     QKHSDVQFNAGGRYGDENGFINDIKNRKSQLFEAQSYDGVAYRTLVNANKENPASTIK
                     PNDPMFNAGRNEIGNAVAGTGNWLQNCNTETSKQTITTHYPDYKEFYCNSPKKDNFNS
                     CTITRDFSVPVYISGGNGDMSMCGDNCVRVWFGRRDDNYWNDGVYDNSLTLKFHPDAK
                     LATAKIINAEWDDHMRVTLDGTQIFAHIDGAYRSSNYPSPQGQWELKKSWKLDKVYDI
                     TEQVKTSVYQEQDREVTMASRVWVGGGGEGYFEVEMTFENMKLEDKHIQEPAGCFDAV
                     QTPNSFCRFDRFVNMDVGTKRLPESVLKMATPLYKGDTGYLTWKTNLEGYFCDPLAKE
                     KLCSYDAKGNIMKDPTGKDLCYNYDDIKNMPDACSTYKNDAACVLDNQTCAEGWFDEG
                     TNTCYMYEQKYTCDRGKDVVREVESQTNSCVGMIPCSGGTCETGPKEENKDFGKVAAY
                     SNMVQYMQGEAKCEDPNDPNSCSVFEGKAEWCGRSVGFVNGLAKTDCCEKPQGAAGSL
                     EAIMLAGSMIRNTNWTRVNAQLVNWTGGESGTWASMANSVGEWTASAGKTVGQMWNNV
                     TSSITSVYENVAGNLGRTVGSSAAGESGQLAKETMSSFGLGKLKQMAMEKAYDLLPDT
                     VRDFVFKNVATTGGEIVFSAAVQNFMLALNVIGWIYTAYQVTKMLLEMLVACDQKEME
                     ASIHKNQKSCFTLDTNRCVKYLNLGFTKKCVKKATDMCCYNSMLSRVIMQQAYPQLGI
                     DPVTSNCVGLSIGQIQKLDFDKIDLTEWINDAVQVGEVPDQYATFSESSITANLPFKN
                     ENYQLPSERTKDAMGGEENMIKARQENAQAIKEENVDCSYLPRPAICEVGSTYVDPIT
                     GKEIPKY"
     gene            complement(3142..4014)
                     /locus_tag="POA77_004382"
     CDS             complement(3142..4014)
                     /locus_tag="POA77_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6367716.1"
                     /translation="MKNKRFVSLVSALVASASLVGCAGNAGQSPNSAQHSDTHGLYWH
                     ANAKDSVAKNAYSLAGFDVSFNEETVQPGGDADNFLSKLLISGSMGYVTGGLSGLSIM
                     SLGSLYSSSDAEYIQVNQYVVFVPNPKKLPYNDESLVRAGAAYVYNHTKESQVMLGFN
                     PSKQSAALASCKIDRAVINKWSTCELTSKPAPDVMPTNSMYSFQAIRPATGTEIPQLN
                     LPAGEYSVIRYVFIPFKGNESSNNFSGIIFRSDSPKFTMPGGAAASINGKDYYLFSGE
                     YGQKGFPEKTLKSK"
     gene            4196..5980
                     /gene="trhI"
                     /locus_tag="POA77_004383"
     CDS             4196..5980
                     /gene="trhI"
                     /locus_tag="POA77_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer helicase TrhI"
                     /protein_id="HDI6367717.1"
                     /translation="MGKPTDEQRVIIENANANNMVIAAPGSGKSFTMIEAVISILRQF
                     PYAKVGMVTFTRAATNSLAEKLKRRLSKKDQDRVLVNTFHGFIRMQLDMVNWKGKMLI
                     SSAQRSVIHRALKESGAPFRYPDAEFAIDAIGREMDTDIISVRHTRQQIHLFNTYQAI
                     CQKDHVADLNALSRFVVGQMYSGKMQPLNLTHLVVDEVQDTDSIQYAWISLHTRAGVN
                     TSIVGDDDQAIYSFRASGGVKIFQQFEKQFRPNIFYLNTCFRCEPEILKVAGALIEKN
                     VYRYAKDLRSAKGGGGKVHFRSYVDMDEQIQGILNLINQDPIGWAILSRGNAHLDQLE
                     SLIEQPVLRYGGKSFWDEKETSDVLHLMAFFRHSNDVRLMKRVLALFGENEEVLDQTA
                     LSMKGRKVTFGELNIPNESSLETRTLHSNFTRFTQETREKVEIEKRFANLIKWMELSS
                     IKMRTQKGSPSLSRIALDTCKQWAEKTGWQNMINRAAAMCLGPKKKDEEYTPDKVVLS
                     TLHGSKGLEWKNVIIMSCNADQIPSKRSVGQEAIEEERRLLFVGFTRAEQQLHVMWYG
                     DPSFFLSECAEDKLKEAAKSRTESPLTE"
     gene            6377..7252
                     /locus_tag="POA77_004384"
     CDS             6377..7252
                     /locus_tag="POA77_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367718.1"
                     /translation="MSKSKARSKALLIAFADLIPDMDKVVNKKLLDSLNVYSGHDNDL
                     IVIMNEDGPTIIELNSLKSVSMLAQKLSAFSTYYHVEMQQILVNPIDFEKAYTLLKEA
                     PAIPMFKTLADLDKFLNEEFEKYGLNTFLDVDNLDYSLAKSRELKNDQLVAWVSEIIE
                     KREKLALRNRFNEVTKAHYETVDAMYAAVRPLMKELGFPDELMLHTFSELSVFDSKGW
                     DYAIKSKIEFLTKREEQCLDYQMKADKRQATVDELLAQINNAKTVKAPRSFGQLFGFS
                     VIAMMTFMFIVNKFI"
     gene            7311..7688
                     /locus_tag="POA77_004385"
     CDS             7311..7688
                     /locus_tag="POA77_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367719.1"
                     /translation="MSYLEYVNEVKVSGIVISAMEKEMTSGNVGLLIKLKNRMKTEIN
                     GEITEREFSIQIKVSPEMYSGCFTGINQGDELMVSGYLVVDTITLEGREHPLDYMRVV
                     ATSKLAHIPKPPKGFGQSSFRQI"
     gene            7749..>7911
                     /locus_tag="POA77_004386"
     CDS             7749..>7911
                     /locus_tag="POA77_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367720.1"
                     /translation="MKQKIRPTAFCSKQMDFFSIFDFEEEEKEIITIVESPLDYINDA
                     IAAQQSIQSE"
BASE COUNT         2508 a         1470 c         1865 g         2068 t
ORIGIN      
        1 tgggcatttt acgtttcgct cataaacatt gcgttaacgc ctaaaatatt tgcggcagat
       61 gcgatttttg accagcttga aaacaatttt aaccttgcca atcccaacgc caaccggaac
      121 gcaaccacca gtgctcagga tattgtggaa aagtataaaa atgctgattc cggggaaaat
      181 ctgagcggca aaatttccga gaaatatgtt ggtaaagcag agtcaacaaa cctaaatgtt
      241 gggaagtacg ggacgccgaa ctcaaatgaa agtgttatga gtaatgcggt gtccgacggg
      301 aaatcgatcg gtaaggctgt acaattacct agcatgtcgg gcggtacaat taactcgaat
      361 tatacgaaag agggggcaaa gttactttct agggacagca gcggtaatat tggaataagt
      421 aacaacccga atacaacggc aggtaccaaa accagtacag gggaactatt cagctcagag
      481 cagaagcaca gtgatgttca gttcaatgct ggtggccggt atggagatga aaatggcttc
      541 atcaatgaca tcaaaaatcg aaaaagtcag ctgtttgaag cgcaaagtta tgatggagtc
      601 gcctaccgta ctctggtgaa tgcaaataaa gaaaacccag catctactat taaacctaac
      661 gaccctatgt tcaatgcggg tcgaaacgaa ataggaaatg ctgtagctgg tacaggaaac
      721 tggctacaga attgtaatac ggaaacatca aaacaaacaa ttactacgca ctatccagat
      781 tacaaagagt tttactgcaa ttcacccaag aaagataact ttaacagttg cacgataact
      841 cgtgattttt ccgtgccggt ttatatctca ggtggtaatg gtgatatgtc catgtgcgga
      901 gataattgtg tgagagtctg gtttggcaga agggacgaca actattggaa cgatggagtc
      961 tatgacaact cattaacctt aaaattccat ccagatgcaa agctcgcaac tgcgaaaata
     1021 attaacgcag agtgggatga ccatatgagg gtcacgcttg atggtacaca gatttttgct
     1081 catatagatg gagcatatag aagtagcaat tatccatctc cacaaggtca atgggagtta
     1141 aagaaatcct ggaagctcga taaagtctac gatattactg aacaagtaaa aacctctgta
     1201 tatcaggagc aggatcgtga agtgactatg gcttcgcgcg tatgggttgg cggtggtggg
     1261 gaaggctatt ttgaagttga gatgactttt gaaaatatga agttggagga taaacatata
     1321 caggagcccg caggatgttt tgatgcagta caaacgccga attcattctg tcggtttgac
     1381 aggtttgtca acatggatgt tggtacaaag cgactcccgg aaagtgtgct taagatggcc
     1441 acaccgcttt acaaaggtga tacaggatac ttaacctgga aaacaaacct tgaaggctat
     1501 ttctgtgatc ccctggcgaa ggaaaaactg tgctcttatg atgcgaaggg aaacatcatg
     1561 aaagacccca caggcaaaga cctttgctac aactacgacg atataaaaaa tatgcctgac
     1621 gcatgcagca catataaaaa tgatgctgcg tgtgtgctag ataatcagac atgtgctgaa
     1681 ggttggttcg atgaaggcac aaatacttgc tacatgtatg agcagaaata tacatgtgac
     1741 agagggaaag acgtagtcag agaggtagag tcacagacta actcctgtgt aggtatgatc
     1801 ccatgttctg gcggaacatg tgaaacaggc ccaaaagaag aaaacaaaga ttttgggaaa
     1861 gtggccgcgt actccaatat ggttcagtac atgcaaggtg aagcaaaatg cgaagatcca
     1921 aacgacccta actcctgctc tgtctttgag gggaaagcag agtggtgcgg caggtccgtt
     1981 ggttttgtga acggactggc caaaacagat tgctgcgaaa agccgcaggg tgctgctggg
     2041 tcactggagg cgataatgct ggccgggtcg atgatccgaa acacgaactg gacccgagtg
     2101 aatgctcaac tggtaaactg gactggaggg gagagcggaa cctgggcatc aatggctaac
     2161 tcagtgggtg aatggactgc atcggcaggt aaaactgtcg gacaaatgtg gaacaatgtg
     2221 accagttcaa taaccagcgt ttacgaaaac gtggccggta accttggccg aacagtagga
     2281 tcatccgctg ctggtgaaag tggtcagctt gctaaggaga ctatgagttc attcgggctt
     2341 ggtaaactta agcagatggc catggaaaaa gcctatgacc tgcttcccga tacagttcga
     2401 gacttcgtat ttaaaaatgt agcgacaacg ggtggagaaa tagtcttttc tgctgctgtt
     2461 caaaacttta tgcttgcatt aaatgtaatc ggctggattt atacggcata tcaagtcaca
     2521 aaaatgctgc ttgaaatgct ggtggcctgc gatcaaaaag aaatggaagc atctattcac
     2581 aaaaatcaga aatcctgttt tacactggat acaaaccgtt gtgttaaata tctgaatctg
     2641 ggatttacaa agaaatgtgt taaaaaagca acagacatgt gctgttacaa ttcaatgtta
     2701 tctcgcgtta ttatgcagca ggcctacccg cagcttggaa tagacccggt tacttcaaac
     2761 tgcgttggct tatcaatagg gcagatccaa aagttagatt ttgacaagat tgatttaaca
     2821 gaatggatta acgatgctgt tcaggtcgga gaagtaccgg accagtacgc tacattctca
     2881 gaatcgtcaa taacagcaaa tttgccattt aaaaacgaga attatcaact tccatcagaa
     2941 agaacaaaag atgcaatggg aggagaggag aatatgataa aagccagaca ggaaaacgct
     3001 caggccatta aagaggagaa cgtagactgt agctatcttc cacgccctgc gatttgtgaa
     3061 gttgggtcaa cttatgtaga tccaattaca ggtaaggaaa taccaaaata ttaaaaaaaa
     3121 ggggctgtaa agcccctttt gttattttga tttcagagtt ttttccggga atcctttctg
     3181 accatactca ccagagaaga ggtaatagtc tttcccatta attgaagcag ctgcgccgcc
     3241 tggcatggtg aactttggac tatcagaacg gaaaataata cctgagaaat tattgcttga
     3301 ctcattgcct ttaaatggaa tgaacacgta acgaataact gaatactcac cggcaggtag
     3361 attaagttgg gggatttcag ttccagtcgc agggcgaata gcctggaagc tgtacatgga
     3421 attagttggc attacgtcag gagctggttt tgaagtgagc tcgcatgtac tccatttatt
     3481 gatgaccgcc ctatcaatct tgcaggacgc cagagctgca ctttgcttcg atgggttgaa
     3541 tccgagcatt acctgtgact ctttggtgtg attgtaaaca tacgcagcac cggcacgaac
     3601 taggctttca tcgttataag gaagtttctt cgggttcggg acaaagacaa catactggtt
     3661 aacctgaatg tattcggcgt cagaggagct gtatagagag cccaaactca taatgcttaa
     3721 tccgctgaga ccgcctgtca catatcccat agagccgcta atcaagagtt tggaaaggaa
     3781 gttatctgca tcaccacctg gctgaacagt ttcctcatta aaagaaacgt caaacccggc
     3841 cagagagtaa gcatttttgg ctacagaatc ttttgcgtta gcatgccagt agagcccatg
     3901 agtgtcgctg tgctgggcgg agttagggga ttgacctgcg ttcccagcac accctaccag
     3961 agacgcactt gcaactaaag ctgaaaccaa ggatacaaac cgtttatttt tcatttatga
     4021 ccctttttga atgaatttta tgtctagaag cattatttca tactcgccag tttctcaaac
     4081 aacctaaatg aagaaaaaat ggaaaaatat gaagtgacca aaaatgagtc ttgtaaagaa
     4141 aaatccgtca aatttaaatc gtataataaa ggggactaaa tattcagagg cttttatggg
     4201 taaaccaaca gatgagcaaa gggtcatcat tgaaaatgca aatgctaata atatggttat
     4261 tgcagcaccg ggatctggaa agtcattcac gatgatagag gcggtgatct ctatcctcag
     4321 gcagttccct tatgccaaag tggggatggt cacatttacc cgtgcagcaa caaattcatt
     4381 ggctgaaaaa ctgaaacgtc gattaagtaa gaaagaccag gatagggtgc tggtcaatac
     4441 attccacggc tttatccgga tgcaactgga tatggtcaac tggaagggca agatgttgat
     4501 tagctcggca cagcgttctg taattcacag ggcattgaag gagtccggcg cgccttttcg
     4561 atacccggat gcagaatttg ctattgacgc gattggaagg gaaatggata cggacatcat
     4621 ttcagttcgc catacgcggc aacaaatcca cttgttcaac acctatcagg caatatgtca
     4681 gaaagaccac gttgcagatt tgaatgcact ctcacgtttt gttgtgggcc agatgtactc
     4741 ggggaaaatg cagccactta acttgacaca tttagttgta gatgaagttc aggataccga
     4801 ctcgattcag tatgcctgga tctcactaca tacgagggca ggtgttaaca ctagcatcgt
     4861 tggggatgat gatcaggcaa tttattcatt cagggcatca ggtggagtca aaatattcca
     4921 gcagtttgaa aaacaattca ggcccaacat attttatttg aatacttgtt tcagatgtga
     4981 accagagatt ttgaaggtag caggggcatt gattgaaaaa aatgtttaca gatacgctaa
     5041 agatctccgt tcagctaagg gcggtggggg aaaagttcat ttccgctcat atgttgatat
     5101 ggatgagcaa attcaaggca ttctcaatct gattaaccaa gacccgattg ggtgggcaat
     5161 cctcagcaga ggtaatgcac atctcgatca gcttgaaagc ctgatagagc agccggtact
     5221 tcgttatggt gggaagtcat tttgggatga aaaggagaca agcgatgtat tgcatttgat
     5281 ggcattcttc cgacattcca acgatgtccg gttaatgaag agggtgctgg cgcttttcgg
     5341 tgaaaatgaa gaggtcttgg accagacagc attgagcatg aaaggaagaa aagtaacttt
     5401 tggtgaactg aacatcccga atgaaagttc cctggaaaca agaacgcttc attcaaactt
     5461 cacccggttt acacaggaga ctcgggaaaa ggttgaaata gaaaaaaggt tcgcaaatct
     5521 gattaagtgg atggagttgt cgtcgataaa aatgaggacg cagaaaggat caccatcact
     5581 gagcagaatt gcgctggata cctgtaagca atgggccgaa aagacaggct ggcaaaatat
     5641 gataaatcga gctgcggcga tgtgtctggg gccaaagaag aaggatgagg aatatacacc
     5701 ggataaagta gttttatcga cgttgcacgg ttcgaagggt cttgaatgga aaaatgtaat
     5761 tattatgagt tgtaatgcag atcagattcc aagtaagcgg tctgtggggc aggaggcgat
     5821 tgaagaagaa agacgactgc tttttgttgg gtttacacga gcagagcaac agctgcatgt
     5881 aatgtggtac ggtgatccgt ctttcttttt aagcgaatgt gctgaagata aattgaaaga
     5941 ggctgcgaaa agtagaacag aatccccttt gacagaataa ttttgatttc tttattacag
     6001 gaatgaaccc gtgtagtata acgtatatga gcttgaattt attgctccca catttcaagt
     6061 aactttataa aagttacttt gcgcgttaag cgcatattca gccgtgaaaa gcggccagct
     6121 ttctaaagct gcaaggcacc accggcaaaa tgtcaccttt gtcgctggtg cctttcccat
     6181 gtctgaaaaa ggacaatcga atttaccaaa tcgtaactaa cttagctctt aattgagcta
     6241 ttttcgttgc gtacttctaa tgccagaaag tataaatcag gcggttcata gcttacggga
     6301 gatgaatgac aggctttgcc tttgtgttgg gatatttaat gattccttcc ttcttactat
     6361 atataggtga ccccaaatgt ctaaaagtaa agcacgcagt aaagctcttc tcatcgcctt
     6421 cgctgatctg atcccagata tggataaggt ggttaacaag aagttattgg attctctgaa
     6481 tgtttattca gggcatgata atgaccttat tgttattatg aatgaagatg gtccaaccat
     6541 aattgaactg aattctttga aatcagtttc aatgctggct caaaagcttt ctgctttttc
     6601 tacttattat catgtggaaa tgcagcaaat tcttgtcaat ccaattgact ttgagaaagc
     6661 ttacacactt ctcaaagaag cacctgcaat tccaatgttt aaaactttag ccgatctgga
     6721 taagttctta aatgaagagt ttgaaaagta cgggttgaac acatttcttg atgtggataa
     6781 ccttgattat tcactcgcta aatccagaga gttaaaaaat gatcagttgg ttgcctgggt
     6841 ttctgaaatt attgagaagc gcgagaaatt agcattgcgt aatcgattta atgaagtgac
     6901 caaagcacat tatgaaactg tagatgcaat gtatgcagcc gttcgcccat taatgaaaga
     6961 gttaggtttt cctgatgaac tcatgttaca cacctttagt gaattgagcg ttttcgattc
     7021 caaaggctgg gattatgcta tcaagtcgaa aattgagttt ctcactaaac gcgaggagca
     7081 atgtttagat tatcaaatga aagcagataa acggcaagcg acagttgatg aactcttggc
     7141 acaaatcaat aacgctaaaa cggtaaaagc accaagatct tttggtcagc tgtttggctt
     7201 ttctgttatt gccatgatga catttatgtt cattgtaaac aaatttatct aatcctgtta
     7261 aggggaaacc ctgacggggc ttctctttgc ttaaattgag gagagaagta atgtcatatc
     7321 ttgaatatgt taatgaagta aaagtgtccg ggatagttat cagtgcaatg gagaaagaaa
     7381 tgactagcgg aaacgttggg ttattgataa agctcaaaaa cagaatgaaa actgaaatta
     7441 atggtgaaat tactgagaga gaattttcga ttcaaatcaa agtatcacca gagatgtatt
     7501 caggctgttt cactggtata aatcaaggtg atgagttaat ggtctccggt tatcttgttg
     7561 tggataccat cacattggaa gggagagagc atcctcttga ctacatgaga gtagttgcaa
     7621 caagtaagtt agctcacata cctaaacctc ctaagggttt tgggcaaagt agctttagac
     7681 agatctgaca tttaaaccac ccaaaagggg agaaatccct tcatgggagt tctcccttag
     7741 gagatcccat gaaacaaaaa ataaggccga ctgcattttg cagtaagcaa atggattttt
     7801 tctctatatt cgattttgaa gaagaggaaa aagaaataat aactatagtt gagtctccac
     7861 ttgactatat aaatgatgca atagccgcac aacaaagtat tcaatcagaa g
//
LOCUS       DAMZII010000187         7853 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZII010000187 DAMZII010000000
VERSION     DAMZII010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.187"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..887
                     /gene="yneK"
                     /locus_tag="POA77_004387"
                     /pseudo
     CDS             1..887
                     /gene="yneK"
                     /locus_tag="POA77_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1037..2227
                     /gene="ydeA"
                     /locus_tag="POA77_004388"
     CDS             1037..2227
                     /gene="ydeA"
                     /locus_tag="POA77_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDI6367721.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2252..2917)
                     /gene="marC"
                     /locus_tag="POA77_004389"
     CDS             complement(2252..2917)
                     /gene="marC"
                     /locus_tag="POA77_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDI6367722.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3129..3563
                     /gene="marR"
                     /locus_tag="POA77_004390"
     CDS             3129..3563
                     /gene="marR"
                     /locus_tag="POA77_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDI6367723.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3583..3966
                     /gene="marA"
                     /locus_tag="POA77_004391"
     CDS             3583..3966
                     /gene="marA"
                     /locus_tag="POA77_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDI6367724.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3998..4216
                     /gene="marB"
                     /locus_tag="POA77_004392"
     CDS             3998..4216
                     /gene="marB"
                     /locus_tag="POA77_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDI6367725.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4273..5712)
                     /locus_tag="POA77_004393"
     CDS             complement(4273..5712)
                     /locus_tag="POA77_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6367726.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSSEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5737..7410)
                     /locus_tag="POA77_004394"
     CDS             complement(5737..7410)
                     /locus_tag="POA77_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDI6367727.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIKSVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7466..7777)
                     /locus_tag="POA77_004395"
     CDS             complement(7466..7777)
                     /locus_tag="POA77_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI6367728.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
BASE COUNT         2088 a         1894 c         1674 g         2197 t
ORIGIN      
        1 aacgacacac tatacaatga aaaggttcag tttaaactca cttatcccgt caagaatgga
       61 agacacaaag aaaatattga atttcaggta gtaataaatt taagccctat ttatttagat
      121 aattttcgcc atgatggaga aattaatatt tttgcgctcc caacccaaag cctgtcacta
      181 tgggacgcgt atttcagacc ggcgtcgagc gtgtgctatt tctgtttctg aatgatttta
      241 ttgaacaatt tccaatgttc aaccttggcg ttcccatcaa aagagcgcat acaccacata
      301 ttgaacccct gcctccggat cgccataccg ctgcagatta cttacgccag tttgatttgc
      361 ttgtcctgaa ttttatctct cgcggtaatt ttgtcatact cccccgatta tggaataact
      421 ctgaggttca cagatggttc gtcaataaag atcctaactt gatcaccgcc attctcgaca
      481 taacagacgg cgaattaaaa gaggatttat tgcaaagcct gatggattca ttaggttcta
      541 acaaacatgt actacccgaa gtctgcatct gctttttatc ccttttagca gaacaagaat
      601 ctcctcattt tcaggacttg tttttatttt tcgccaatat gttattgcac tatcaccaat
      661 ttatgaatcc caatgaaagt gatttgaatg acgtgttgat gccagcatca ttaagtgatg
      721 ataaaattat caaacatatg gcgcgcagga ccctcaaact gtttgtaaaa aatgaaacgc
      781 cgccaaaagt tactcacgaa gacctggtga aaaacaggcc tcgctcccct gtcagaccac
      841 ctatacccgc aaccgccaaa acgccagacc tccctgaacg tcattaaacc gtgatgttac
      901 cgactctctg acgcggaaca gaatcagcgt cagagaaacg gaaaacgcga tccagatcac
      961 aaatgcattg tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact
     1021 cacccgataa tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt
     1081 cgttacgctg gcagtcgccg ccttcatctt caacaccacc gaatttgtcc ctgttggcct
     1141 gctctctgac attgcgcaaa gttttcacat gcaaaccgct caggtcggca ttatgttgac
     1201 catttatgca tgggtagtgg cgctaatgtc attgcctttt atgttaatga ccagccaggt
     1261 tgaacggcgc aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc
     1321 gtttttatcg tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca
     1381 tgcgattttc tggtcgatta cggcgtctct ggcgatccgt atggctccgg ccgggaagcg
     1441 ggcacaggcc ttgagtttaa ttgccaccgg tacagcactg gcgatggtct taggtttacc
     1501 tctcgggcgc attgtgggcc agtatttcgg ttggcgaatg accttcttcg cgattggtat
     1561 tggggcgctt atcacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca
     1621 ttccggttca ttgaaaagcc tcccgctatt gttccgccgc ccggcattga tgagcattta
     1681 tttgttaact gtggtggttg tcaccgccca ttacacggca tacagctata tcgagccttt
     1741 tgtgcaaaac attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg
     1801 tggtgcgggc attattggca gcgtgatttt cggtaaactg ggtaatcagt atgcgtctgc
     1861 gttggtgagt acggcgattg cgctgttgct ggtgtgcctg gcattgctgt tacctgcggc
     1921 gaacagtgaa atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat
     1981 cgggcttggt atgcaggtta aagtgctggc gctggcacca gatgctaccg acgtcgcgat
     2041 ggcgctattc tccggcatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca
     2101 ggtgagtttg cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc
     2161 cgcgttaatt tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac
     2221 gcaatagttg aaaggcccat tcgggccttt tttaatggta cgttttaatg atttccagga
     2281 tgccgttaat aataaactgc acacccatac ataccagcag gaatcccatc agacgggaga
     2341 tcgcttcaat gccacccttg cccaccagcc gcataattgc gccggagctg cgtaggcttc
     2401 cccacaaaat aaccgcaacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat
     2461 cagcgaaggt tgaactctga cgcactgtgg acgccgagct aataatcatc gctatggttc
     2521 ccggaccagc ggtacttggc attgctagcg gcacaaaggc aatattggca ctgggttcat
     2581 cttccagctc ttccgacttg cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa
     2641 agagcatccg aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa
     2701 tcgaaatgcc aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg
     2761 caaatacgta caccgaggcc atcaacgact gacgattacg ttcggcactg ttcatgttgc
     2821 ctgccaggcc aagaaataac gcgacagttg ttaatgggtt agctaacggc agtaacacca
     2881 ccagccccag gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc
     2941 tgggttatca gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac
     3001 cttgcatcgc attgaccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca
     3061 tttatttgac ttatacttgc ctgggcaata ttatcccctg caactaatta cttgccaggg
     3121 caactaatgt gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa
     3181 tccatatggt taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata
     3241 ttaccgcggc acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg
     3301 ttgaactgaa aaaagtgttg tcggtcgacc tgggagcact cacccgtatg ctggatcgcc
     3361 tggtctgtaa aggctgggtt gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg
     3421 taaaacttac caccagcggc gcggcaatat gcgaacaatg ccatcaatta gttggccagg
     3481 acctgcatca agaattaaca aaaaacctga cggcggacga agtggcaaca cttgagcatt
     3541 tgcttaagaa agtcctgccg taaacaaaaa agaggtatga cgatgtccag acgcaatact
     3601 gacgctatta ccattcatag cattttggac tggatcgagg acaacctgga atcgccactg
     3661 tcactggaga aagtgtcaga gcgttcaggt tactctaaat ggcacctgca acggatgttt
     3721 aaaaaagaaa ccggtcattc attaggccaa tacatccgca gccgtaagat gacggaaatc
     3781 gcgcaaaagc tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc
     3841 gagtcgcaac aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa
     3901 taccggatga ccaatatgca gggcgaatcg cgctttttac atccattaaa tcattacaac
     3961 agctagttga taacgtgaca acgtcactga ggcaatcatg aaaccacttt catccgcaat
     4021 agcagctgcg cttattctct tttccgcgca gggcgttgcg gaacaaacca cgcagccagt
     4081 tgttacttct tgtgccaatg tcgtggttgt tcccccatcg caggaacaac caccgtttga
     4141 tttaaatcac atgggtactg gcagtgataa gtcggatgcg ctcggcgtgc cctattataa
     4201 tcaacacgct atgtagttat tgtcaggctc tgatcacaga gcctgacatc tccgtaatat
     4261 cttcctcaag tctcaaatat tttcgccatt gttggcgatg agatcccgat accagtaaaa
     4321 cgacgcttta cgtatccgtt ccagcgttcc cttcccttcg ttgtctttat cgacataaat
     4381 cattccgtag cgctttttca tttcccctgt tccggcagaa accaggtcaa tgcagcccca
     4441 cggggtgtag ccaattaagt caacaccatc ttcaactacg gcttttttca tttcccgaat
     4501 atgggaagca aagtaatcaa tgcgatagtg atcgttcacc gtgccgtcag cttgtctctg
     4561 gtcaaccgca ccaaatccat tttcgacaat aaacagcggc aactggaaat gatcccagaa
     4621 ccagttgagt gaataacgta gccctgccgg atcaatttgc catccccatt cggatgtatc
     4681 gatataaggg ttgctgacca aatctcgtgg ttcaacataa tccagttgcg ggttatcggg
     4741 cgaaaattgt gtcgtaaaag acatgtagta gctaaagccg ataaagtcga cacaaccact
     4801 ggcaagaatc gcgttatctt ctggtgtgat atcgaggttg aatcctttcc tggcaaagta
     4861 attcagcata tgttgcggat aatatccacg agcatgaaca tcagtaaacc agtaacgacg
     4921 atgcattgct ttcgtggcca tcatcatatc gttgggtgca cacgtcagag gatagatggg
     4981 acacatggcg atcatacagc caatgttaaa ttcaggattg atcgattttc cagtctgtac
     5041 cgccagggca ctggcaacta actcgtaatg taccgcctgg tacattattt gttcgcgctc
     5101 attgatatct tcctctgacg aatacaagat accggaatta gtaaatggac acaggctttc
     5161 gctgaaattc acctgattat tgatttcgtt aaacgtcatc cagtacttta ctttttcttt
     5221 atagcgcgtg aagaccgttg atgcgaagcg gataaaaaag tcgatcagtt tacggtttcg
     5281 ccagccacca tattttgtca ccagatgata aggcatctca aaatgtgaaa gcgtcaccac
     5341 aggttccatt ccctgcttaa ggcattcatc gaacagatca tcataaaatt gtaaaccctc
     5401 ttcattcggc tcctgttcgt caccttgcgg aaagattcgt gtccaggcaa tggaagttcg
     5461 aaagcatttg aatcccatct cggcaaataa ctgaatatct gttttatagc gatgataaaa
     5521 atcaattgct tcatgattgg gataattaag cccgtcgata acgccttctg tcacttcacg
     5581 cggcaccccg tgagcgccag cagtcatcac atcagcgatg ctgatgcctt ttcctccttc
     5641 attccagcca ccttccaact gatgcgcggc taccgcaccg ccccataaaa aacctttttt
     5701 aaatcctgac atacacaact ccttaactaa aatagattac caccagactt ccgcctggat
     5761 gccggccata aactcgtctt tgctgttctc attaaactta aaattcgata attcgttatc
     5821 cagaatattg atatacgttc catagaagcg aatttctggt cgtgaaccta aaatgctttc
     5881 acccactttc aatgcatgcc agagtgtcgt tttataagcc gattcattta gtgttacacc
     5941 ctgctgagtt ttgttctgtt gcttaaacca gcctaattca agccccgtct gattccatgt
     6001 attccagatc caggccggtc ttattacagt gcgaatactg tcaaaatcac tatgagcacc
     6061 actttcataa ctataaacat cttcgccgtg agaatagaca agcgcgttag ccataataat
     6121 attgtcagta agatacatct cgccctgaga gattaaacgc caggcgatcc cattggtatg
     6181 atcaccatag taatagcgac catgcgccat cgtattactg gcatctgaga aactggcaaa
     6241 actgctggca taggaattat tggcaacctg taacgtaaat tcattaaacg tatcgcgttg
     6301 caggttttgc cgtaaaacag aagtcagcat ccagctatct ttcatttcaa aataactgtt
     6361 gtcattttcg ttatcttgtt gttgatccgt tttattaggt gcggaatatt tagccattaa
     6421 tgataatgtc gcatcatccc ataacgggat attgcgataa cgaacatcca cagaattggt
     6481 attcatctgg ctggtacgcg aaaaatcacg agagtaaaca tcgacatcat cacggcttaa
     6541 ggacatatca aacagcccta caccaagtgc ccagttttca atccccacac ccgcggcgac
     6601 atccgtggtt aaggttttcc agtccagcat ctggatctca tattgcggga gtttatgttt
     6661 gccgacccag aagtctgcct ctggtgcgaa gggtaaaaaa cctcgcgttg tcaggtaaat
     6721 atcgctgaac tgcatgatat tttcattggc actgtcacca aaccaggcat cgttatactg
     6781 ctcacccacg ttgccatcat aggtaacgac cgcatttgcc gttttaccgt cctgattata
     6841 aacacgctga tttaaggtca ggtcaaacca accgctcatc tcattaccaa aacgtcccag
     6901 agaacccgct gcataagttt gtggtgagcc gtgattacca gtaccccagc ctgagcggaa
     6961 gtacccctga tagctgaacc caatatcatc ctttatatat ttactgatat ctttcagggt
     7021 cacgcttttg atagtggttt tgtcgccttt actttcaata acggcaactt gctgcgatcc
     7081 atttatagta tgcgctgcgg cagacgagtt ctgttcaccg ttttcatttt tgatgttatc
     7141 agcgacggga gatgtcgctg atatttcagc aagcgaggct gatttatatt tgttttccgt
     7201 gatgcttttt tgtaaggtcg aaagtcggga tttatatact cccagttcat tctgtgttgc
     7261 tttcagctct tgtttatttt gcgacaattc attttcaagc aattcaaggc gctgctctat
     7321 ggttaatttt gttgccatcg cgttcaatgg aaacaatgct gtaataatag aaaaagacaa
     7381 aaggctgacg ttaagcgtct ttatattcat gacttatcct taagagtata ataatttcct
     7441 ccagctatcg cactggagga aaaaatcagg catggagttc ttttcttaat gaaataattt
     7501 ccctggctaa atcctggcat aatattgcat tcatcaaatg atcctgtgca tgaatcagaa
     7561 ttaaattaac ttttattttg ccacagcctt catcctgact aattaatgcg gtctgaatct
     7621 tatgtgcctg aagacaggca gattcagaac tggccattag ctgagttgcg ccctgccagt
     7681 ctttttgccc ggcacaacgt atggcttcca tcgcgtcaga acgtgcctgc cccgcgttga
     7741 ccagtaactc cataacaagt tcttcatctg caaacatcac aatctccttc ctgggtaaat
     7801 ggaattatcc gttagttgtc atgttattga ctttgttggc ggcaataaca aaa
//
LOCUS       DAMZII010000188         7782 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZII010000188 DAMZII010000000
VERSION     DAMZII010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.188"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            106..822
                     /gene="nanC"
                     /locus_tag="POA77_004396"
     CDS             106..822
                     /gene="nanC"
                     /locus_tag="POA77_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDI6367729.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVAFKL"
     gene            842..1948
                     /gene="nanM"
                     /locus_tag="POA77_004397"
     CDS             842..1948
                     /gene="nanM"
                     /locus_tag="POA77_004397"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDI6367730.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2013..2993
                     /locus_tag="POA77_004398"
     CDS             2013..2993
                     /locus_tag="POA77_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDI6367731.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERYGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(3001..3123)
                     /locus_tag="POA77_004399"
     CDS             complement(3001..3123)
                     /locus_tag="POA77_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6367732.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3205..4077
                     /locus_tag="POA77_004400"
     CDS             3205..4077
                     /locus_tag="POA77_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6367733.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            complement(4140..5450)
                     /locus_tag="POA77_004401"
     CDS             complement(4140..5450)
                     /locus_tag="POA77_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDI6367734.1"
                     /translation="MKSSSHFTVFEYGHLVSEEGDARINNAVVLPADLFKWLELRCLK
                     DDADNTHRLLTLSSRNGVKTLQVKNYAGVISLPGGRFLEILPKTGQDNEDTVRQQLLM
                     MLRTLKSFRHIASSESGVKISKMPLMDIFIQQFIESVRKIVQRGLKRDYLRQEDNLPW
                     IKGRLRISAQLSKNCIRRDLFQVEYDEYSVNRLENRILKTAINKISRQTSNPQLLQQI
                     TQLQFHFENITSVHDAYIAFEQLHFDRHMHHYEQALAWAKMILLGDSPHCMYGDVNAF
                     SLLFPMEAVFESFVTTWMRYRYYDKWRVDAQVSSKNLISYNGKALFKLRPDICLRPRK
                     STTGSVITCDVKWKIVNGRKDSLEQSQADLYQMLAYGLNYQEGEGDMILIYPYHNGFN
                     QPSPHPYEFSHQKENRLRLWVVPFFIGESLQTSELRFPSGAGFI"
     gene            complement(5447..7501)
                     /locus_tag="POA77_004402"
     CDS             complement(5447..7501)
                     /locus_tag="POA77_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HDI6367735.1"
                     /translation="MVQDSSALAPFFRAQIEKNNQYNSVWGKHYRQLVEQIQAEKPNF
                     SEATIRQVWYERDNGVSSLMQGGMSLVEFENAQKDLLTLTRQIAEECTQENYNRVIER
                     LIKLKADGVLNKVYRALCNRAFAAIYPSKISSVVNVNNFFSCYNYCNNHFQLGLSGER
                     EWLARNVDLKETLNRALGDDIDPIELNMSLWHLYAYEIQDKNAIGLVGSESALQENEQ
                     EDDVISSPLPKNTILYGPPGTGKTYRTIEIAVQICDPIAYSLQEGKDESEKRRELKKI
                     YDQLIKDNRVRFITFHQSFGYEEFIEGLRAETTDDGNVKYEIKAGIFKQICEDAAFGH
                     AGAQEMLDSALTRLQERLSEGENITLETQQGKAFQLTYKSQTNFGIFPSQSKKEDLGQ
                     GYNAYLKDIRQVYQDPRAKVHNPSYVRSILNYLINQRYLPLNTQSVASEKKENYVLII
                     DEINRGNISKIFGELITLIEPSKRAGEPESLSVLLPYSSTPFSVPNNLYLIGTMNTAD
                     HSLTALDTALRRRFDFEAMLPDITVLKDTVIKGIDLPRLLQTLNDRIQVLYDREHMLG
                     HAFFIPVVQAKEDEALAFERFKRIMRNKILPLLEEYFYNDWQKIRMVLADNQKEEAPD
                     LQFVREVKYQKKYADLFGDNGTDDLGTSFHLAAEDDNVWDNPIAWQQIYAPQKEKRRS
                     AE"
BASE COUNT         2263 a         1681 c         1662 g         2176 t
ORIGIN      
        1 gccaaagaca ctataagcct ggcaaaaaaa tattacacaa cataaatgct aattgtttat
       61 gcgggctttg tattgctttc tgtatcctac aaatgagtga aatttatgaa aaaggctaaa
      121 atactttctg gcgtattatt actgtgcttt tcgtcaccat taatttctca ggctgcaaca
      181 ctggacgtac gtggtggata tcgtagtgga agccacgcct atgagactcg actcaaagtc
      241 agtgagggat ggcaaaatgg atggtgggca agcatggaaa gtaatacctg gaataccatt
      301 catgataata aaaaggaaaa tgccgcactc aatgatgttc aggttgaagt taattacgcg
      361 attaaacttg atgatcaatg gacggtgcgc ccgggaatgt taacgcattt tagcagcaat
      421 ggcacacgct acggacccta cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt
      481 ggcattcgct atcgttacga ctggaaagct taccgacaac aagacttatc cggtgatatg
      541 tctcgtgata acgttcatcg ttgggatgga tatgtcactt accatattaa tagtgatttc
      601 accttcgcat ggcaaacgac gctatacagc aaacagaacg attatcgcta tgcaaaccat
      661 aagaaatggg cgacggaaaa tgcatttgtt ctacaatacc atatgacgcc cgatattacg
      721 ccatacatag aatatgacta cctggaccgt cagggtgttt acaacggcag agataattta
      781 tcggaaaaca gttatcgcat tggtgtggca tttaaactgt agtagacagg agacagtcac
      841 aatgaataaa acaataacgg cgcttgctat cctaatggct tcatttgccg caaacgcgtc
      901 tgtattaccg gaaactcctg taccatttaa aagtggtacc ggagcaattg ataacgacac
      961 tgtctacatt ggtttaggta gcgcaggtac ggcatggtac aagctggata cacaggccaa
     1021 agataaaaaa tggacagcgt tagctgcatt ccctggcgga ccaagagatc aagcaacctc
     1081 tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt ggcaaaaata gcgagggctt
     1141 gactcaggta tttaatgacg tacacaaata caaccccaaa accaatagtt gggttaaatt
     1201 gatgtcgcac gcaccgatgg gcatggcggg tcatgtaact tttgtacaca acggcaaggc
     1261 ttatgttact ggcggtgtta accagaatat cttcaatggc tattttgaag atctcaacga
     1321 ggctggaaaa gattcaaccg ctgtagataa aatcaacgct cactattttg acaaaaaagc
     1381 agaagattat ttcttcaata agtttctgtt gtcttttgat ccctcaacac agcaatggag
     1441 ttacgctggc gaatccccct ggtacggaac ggctggtgcg gcggttgtga ataaaggtga
     1501 taaaacctgg cttattaatg gcgaagccaa accaggattg cgaacggatg ccgtatttga
     1561 acttgatttc accggtaata atttaaaatg gaataagctt gctcccgtct catcaccaga
     1621 tggcgtcgct ggcggttttg cggggataag caatgattct cttatctttg ccggaggggc
     1681 cggattcaaa ggttcacgag aaaattacca gaacggtaag aactatgcgc atgaaggcct
     1741 gaaaaaatca tatagcgctg atattcatct ttggcataac gggaaatggg ataaatcggg
     1801 tgaattatcg caaggtcggg cctacggagt atcattgccc tggaataata gtctattgat
     1861 tattggcggt gaaactgcag gcggcaaagc ggtgacggat tcagttttga tctctgtgaa
     1921 ggataataaa gtcacagtac aaaactaacg cttcagggcc ccagtaaagg ggccctgcta
     1981 tcaatttatc atttcaatta cgcgaaaatt atatggacgc aataatatcg cccgattatt
     2041 actatgttct taccgttgct ggtcagtcta atgctatggc gtatggcgaa ggactgccat
     2101 taccggacag ggaagatgcg cctcatccca gaattaaaca attagcgaga tttgcgcata
     2161 cgcatcccgg aggcccgtca tgtcacttta acgacattat tccactgact cactgcccac
     2221 acgatgttca ggatatgcag ggttatcacc atcctctggc aacgaatcat caaacacagt
     2281 acggcaccgt tggccaggca ctgcatattg cacggaaatt actgcccttc attcctgata
     2341 atgcagggat tctcatcgtt ccgtgttgcc gtggcggatc ggcttttacc gcaggcagcg
     2401 aagggacata ttcagaacgg tacggagcca gccatgatgc ttgtcgttgg ggaacggata
     2461 ctccgctata ccaggattta gtcagcagaa cgcgagccgc actggcaaaa aatccgcata
     2521 acaaattcct cggcgtatgc tggatgcaag gcgaatttga cttaatgacc agtgactacg
     2581 cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat
     2641 accattctca gcttaataat attactgacg cgccgtggtt ttgcggcgat accacctggt
     2701 actggaaaga aaatttcccg cattcgtatg aagctattta tggcaattat caaaataatg
     2761 ttttagccaa tattattttc gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg
     2821 cgcctgatga agatccggac gatttaagca cgggatatta cggttcagcg taccggtcac
     2881 cggagaactg gacgacggca ctgcgaagca gtcatttcag cacggcagcc cgtcgggggg
     2941 ttatttctga caggtttgta gaagcaattt tgcagttttg gcgcgaaaag tgagcgttca
     3001 ttaatttgat ttattcgcat ccatcaacgc ttttaactgc tccggcgtaa acggtggttt
     3061 gcgccggtgc agcatcgcat ggcaattagg gtacacaggc agcaaaaaaa caaacgagat
     3121 caatgtaata tatcttcact tgcaggcttg aacagcagaa aatttgcgag catgatcgca
     3181 attatttcac tgccagggat atcaatggct tccagtaaat cgctacagca ggcaattgcc
     3241 aatataaaaa tctggcataa gggtgaacag cgcgcaccgc ataaaccatt gttattgcta
     3301 tacgtattag cgggatacct gaatggacat ccacgccttt tcgattatgg atcggaaatc
     3361 tacgaacctc tgcacagttt actggaacgt tttgggccac agcgttcaca gtaccgacct
     3421 gatatgccgt tctggcgatt acaaggcgac ggattctggc aattacacaa tgccgagctt
     3481 tgctcaacag cagggagcag ccgacaaccg ccagtgaagg aactgactga ataccacgtt
     3541 gcaggcggtt tcgatgaaca gcactacgcc ctggtaaccg gcaataaaaa actcatcaat
     3601 actctggcgc aacagatact cgaagcgcac ttcaccgaga gtattcagga agaaattgct
     3661 gacgagctgg gttttgatct ccagcaaatc cgtaaacagc gcgacccgtt atttcgcaaa
     3721 aatgtattac gcgcttataa ctaccagtgc gcgatatgtg gctttaatat gcgccacgac
     3781 gacaccaccg tcgcgctgga agcggcgcat ataaaatgga aacagcacgg cggtccttgt
     3841 gaaattccta atggcctggc gctttgcgca attcaccata aagcctttga taaaggatcg
     3901 attggcctgg acgaaaatat gcgggtactg gtatcagatg cggtaaacgg cggagggatc
     3961 gttgagagat tattctggga ttttgacggg aaaactattg cgttgccgca ggtgcgtaaa
     4021 aattaccctt atgaggtgtt tgtagagtgg catcggaaag aggtttttag ggggtgaggt
     4081 aggctatctt gtgcatacaa atagacaaat aaattgtata aagagatagc ctacaaatac
     4141 taaataaatc cagccccact aggaaaacgc aactctgatg tctgtaaact ctcgccaata
     4201 aagaaaggta caacccataa acgtaaccta ttttccttct gatggctaaa ttcataagga
     4261 tgaggagatg gctgattaaa tccgttatga taggggtata tcaaaatcat atcaccttcc
     4321 ccctcctggt aattcaatcc ataggcaagc atttggtaga gatcggcttg agattgttca
     4381 agactgtcct ttctgccatt tactatcttc catttaacat cacaggtaat aactgaacca
     4441 gtagtacttt tacgaggccg taaacaaata tcaggccgca gcttaaataa agctttaccg
     4501 ttataactaa tcagattttt tgaacttacc tgtgcgtcca ctcgccattt atcataataa
     4561 cgataacgca tccatgtcgt cacaaaagat tcaaaaacag cctccatcgg gaaaagcagt
     4621 gaaaaagcat taacatcccc atacatacag tgtggagaat cgcccaataa aatcatcttc
     4681 gcccacgcta atgcttgctc atagtgatgc atgtgtctat caaagtgtaa ctgctcgaaa
     4741 gctatataag catcatgaac tgacgtgata ttttcaaagt gaaactgcag ctgtgttatc
     4801 tgctgtaata attgtggatt actggtctgg cgactaatct tattgattgc agttttgagt
     4861 atccggtttt ccagacgatt gacgctgtac tcgtcatact ccacctgaaa aagatcccgc
     4921 ctaatacagt ttttgcttaa ttgtgcagag atccgtaatc ttccttttat ccatggaaga
     4981 ttatcttcct gacgtaaata gtcacgtttt aaccctctct gcacaatctt acgcacgctc
     5041 tctataaatt gttgaataaa gatatccatc agaggcattt tagatatttt gacgccagat
     5101 tcagaggagg caatatgacg aaatgatttt aacgtacgta acatcatcag taattgttga
     5161 cgaacagtat cctcattatc ctgacctgtt ttaggcagga tttcgagaaa tcgcccccct
     5221 ggcagtgaga ttactcctgc atagtttttt acctgcagcg tttttactcc attccggctg
     5281 ctaagggtga gcaaacgatg agtattatca gcatcgtctt tcagacagcg cagctcaagc
     5341 catttaaata ggtctgcagg tagcactacc gcattattta tacgggcatc accttcctca
     5401 ctcactaaat gcccgtattc gaacacagtg aaatgactgg aagatttcat tccgcactcc
     5461 tgcgtttttc tttttgaggt gcataaattt gctgccaggc gattggattg tcccaaacgt
     5521 tgtcatcctc tgctgccagg tgaaaactgg ttcccaagtc atctgttccg ttatcaccaa
     5581 agagatcggc atactttttc tggtatttca cctcacgtac aaattgaaga tctggtgctt
     5641 cctctttttg gttatcagcc agtaccatgc gaattttttg ccagtcattg taaaaatatt
     5701 cttccagcaa tggtagtatc ttatttcgca taatccgttt gaatctttca aatgccagag
     5761 cttcgtcttc ttttgcctgc acaacaggga taaaaaaagc atgccccagc atatgttctc
     5821 tatcgtataa aacctggatg cgatcgttaa gcgtctgcaa tagacgaggc agatcaatac
     5881 ctttaatgac agtatctttc agaacagtaa tatctggcag catcgcttca aaatcaaatc
     5941 tgcgacgtag tgcagtatca agtgcagtaa gcgaatgatc agcagtgttc atggtaccaa
     6001 ttaaataaag gttattcggt acactaaatg gagtggaaga gtatggcagc aatacgctaa
     6061 gcgattctgg ctcaccagcc cgtttagatg gctcaataag ggtaattagt tcaccaaaaa
     6121 tttttgaaat attaccccga tttatttcat caataatgag aacatagttt tccttttttt
     6181 ctgaagctac gctctgagta ttcagaggca gataacgttg attaattaaa tagttaagga
     6241 tactgcgtac ataagatgga ttatgtactt ttgctcgtgg atcctgataa acttgccgaa
     6301 tatccttcaa ataggcattg taaccctgtc ctaaatcctc ttttttcgat tgtgacggga
     6361 aaattccaaa gttggtttga cttttatacg tcagctggaa ggccttaccc tgttgtgttt
     6421 ccagcgtgat attttcacct tcagataaac gttcttgtag gcgagtaagt gcgctatcca
     6481 acatttcctg cgcaccagca tgaccaaagg cggcatcctc gcatatctgt ttaaaaatac
     6541 ctgcctttat ttcgtatttt acattaccgt catcagtagt ctcagctctt aaaccttcaa
     6601 taaactcttc ataaccaaag ctctgatgaa aggtgataaa acgcactcga ttatctttga
     6661 taagctgatc ataaatcttt ttcaactcgc gacgtttttc actctcatcc ttaccctcct
     6721 gcagggagta agcgatcgga tcacaaattt gtacagcaat ctcaatagta cgatatgttt
     6781 tacctgtacc tggcgggcca tagagaattg tgtttttagg caatggcgaa cttataacat
     6841 catcttcttg ctcattttct tgtaaagcac tttcgcttcc aactaaaccg atagcatttt
     6901 tatcctgaat ttcatacgca tagagatgcc aaagtgacat attcagctca atagggtcga
     6961 tatcatcacc cagcgcacga tttagcgttt ctttaagatc aacattgcga gccagccact
     7021 cccgttctcc ggaaagacct agctggaaat ggttgttgca ataattgtag caactaaaaa
     7081 aattattaac attcacgaca ctgctaattt ttgatgggta tattgctgca aaagcacgat
     7141 tacaaagagc cctatatacc ttatttaata ctccgtccgc tttaagtttg ataaggcgtt
     7201 caatgacccg attataattt tcttgtgtac actcttctgc tatctgacgg gtgagcgtta
     7261 gaaggtcttt ttgtgcattt tcaaattcaa ccaatgacat ccctccttgc atcaggcttg
     7321 agacaccatt gtcacgttca taccatacct gccgaatcgt ggcttcagaa aagtttggtt
     7381 tttcggcctg aatctgttct acaagctggc ggtagtgttt gccccaaact gaattgtatt
     7441 gattattttt ttcaatttga gcacgaaaga agggggctaa tgctgatgaa tcctgaacca
     7501 tctcttaatc caatatgtag gggggaggga aattgggata gccttatctt atcggattaa
     7561 tctcaaaaat gttaaatttt gtgataaaaa aaataacttt catcctgaaa ttaccttctg
     7621 ccctggagcc accacattta tctgatgact taactgcaaa acgcatgatg ttgatggctc
     7681 tcagagaagg ctatagcatt ctttcatacg taaaatcgct actcgttttt caacctgtgt
     7741 gaggtttcta gttgcatagg gaatcaactg tttttaggta aa
//
LOCUS       DAMZII010000189         7601 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZII010000189 DAMZII010000000
VERSION     DAMZII010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.189"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(85..4947)
                     /locus_tag="POA77_004403"
     CDS             complement(85..4947)
                     /locus_tag="POA77_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
                     /protein_id="HDI6367736.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWL
                     YRYDERGLFSNITDPAGQTWTQLCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEEHDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELAEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETERYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGL
                     REKFMMGLTN"
     gene            complement(4967..5428)
                     /locus_tag="POA77_004404"
     CDS             complement(4967..5428)
                     /locus_tag="POA77_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDI6367737.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5456..7357)
                     /locus_tag="POA77_004405"
     CDS             complement(5456..7357)
                     /locus_tag="POA77_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI6367738.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1456 a         2292 c         2010 g         1843 t
ORIGIN      
        1 ttttaatata aggaatttcc cttaactcta actcacgccg agccaagtca agagccattt
       61 cgatagttat tttgttatgt tttatcaatt tgttagtccc atcatgaatt tttctctcaa
      121 tccctcaaca tattttatgg catgagctat ttccgcttca ttgaataaat ttttactttt
      181 taatatttca tttagaacgt gttcctccct agataaacgt cctagtgctt cgtatgcttc
      241 tcgtccaatt ttcgcaaatt gcttagcatg aatcacttca tgatacacct catataaggt
      301 agcatttttc ctaagataaa tattgcgaag gccataatca aaagctgccc tagcatttgg
      361 tggcaaatat ttatctgctt tccttattac tttaattcct agcttctcca tttctttagc
      421 aatattgttt atttgactac gtgataaacg aaccccatcc aggaaacccg tatgcccact
      481 aacacgacga ccatcgccaa tatagtccat caaactgctg attttagaac ttgcagccaa
      541 tcccagcgga tcaacccacc cacaaggatt cggcacatac cggtaagtct gctccccgcc
      601 cagcaacccc agcggatcac tcaccaggta cattccggtt tccggctcgt aataccggaa
      661 ccggttatag cacagccctg attccgcatc ctgccactgt cctgcataca gcaggccggg
      721 gtcggcttcc gggtccagtt ccccgcgcgg gtccgggtag ccggtgtctg cgggcagact
      781 gagtgccagc ccccacaggc tggtctgccc cggtcgccag acggttttac cttcactgtt
      841 aaagagcatc agcgggcggc ccgtcaggtc gctcaccgcg tgattcgtcc gcgtcaccca
      901 ctgcggggcc acggacgggt gcggctgccg tacccggctg aactgctggc ttatcagctc
      961 aaagccgtta aacaccaggt gccttacgct gtacagttta tcatcgcggt attcccggat
     1021 ttccgcaata ctgtcgccgt cccacagata cgtgaagcgg attttcttcc ggtcgcagcg
     1081 tttctctgtt cgtctgccgc tggcgtcgtg gcggtattcc cactgctcac cctgtgcgct
     1141 gcaatacccg gtcagctggt cacggctgtc ccaccggaac cgctctgttt ccgggcgata
     1201 gccgtcacgg tgttttgtcc ggctgaccat ccggcctgcg gcgtcatagt catactgcgt
     1261 gtttccggcc tgtttcagcc ggtggcctcc ggcataccgc tcagtctctt cacttatccg
     1321 gtgcctgccc gcagactgcg ccttcaggta gccacaactg tcatagccat aggactcatc
     1381 gccttcgcca tagcccagcc cggtgcctga ggctgtcacc gccgtgacct ggccgttccg
     1441 gtccagccgg tattcccgcc gtgtttcccg tccgtaatct tcccggctgg tgacgcctga
     1501 cacgttaccc gcgctgtcgt aacggtactc ccgtaccagt gagggaacca gcccgcccca
     1561 tgtggcttct tccggaaaaa atccggcgcg ctggctgaca ggctgaccgg ctgcattcca
     1621 gccctgttca caggcaaaac cccggttact gtgccggagc acttcacgtc ccgcctcatc
     1681 acggctgaac gtcagcgtct catcccccgg caggataacc tctgccagtt ctccggcggc
     1741 gttgtaacgg taggtggttt tcagcaggga ttcatcggga tgtctgtcgc gttcatcttc
     1801 cggtggcagg atacagcgga tgacggtgtg ttcatcgggg tagtggtatt caatggcccg
     1861 tcggccctga gtggtgacat tcacccggcc gcgttcgtcg tagccggtgc gctgtgatgt
     1921 gccgtccggg ctgaagatac ccgtcagcct gccggatttg tcataggcat actgcgtttt
     1981 cccttccggc gagatgactt ctgccaggcg gcctgcggca tcccggctgt agaggatggt
     2041 ttcctccagg ccgttaaccc tggcggtaca gaggccatcg gcgtcatagc ggtaacgcca
     2101 gaccacgccg ccccagtcat gctcttccgt cacccggccg cagttatcca gaacataaag
     2161 atatgattca ccctgcgggt taatgacttc tgtcagttgc agcgtgtctt tatcgtagcg
     2221 gtactgccag cggtggcctt caccgtccgt tcgcgccacc ggcaaatcga acgggccgta
     2281 ctccattgtc cagacaacac cattgccgtc cgtgtacgcc agcaggttgc cgtggcggtc
     2341 atagcgaaat ccttccgcgc cggttccagc acgttcaatg ccgctcagtt tatggtgctg
     2401 ctgccactgt aaccgcactg actgaccgcc ggggccgcgc atcttcaccg gcaggtcgtg
     2461 accgctgtat tcggtgtggt gttcacggcc catcacgtcg gtgagtgttt ccggccggtt
     2521 gtggtggtcg tactctatgc ccagacgccg ttcatcctgg cggaatattc ccgacaacag
     2581 cccctgagcg gtgtacgcca gccgggtctc ttcgccctgt ggcgacacca ggcttaccgg
     2641 caggccgcgt tcatcgcaca gctgtgtcca cgtctgcccg gcagggtcgg tgatgttgct
     2701 gaacaggccg cgctcatcat agcggtacaa ccactccgcg ccgtctgccg ccgtgtaacg
     2761 gcttaactga ccgtgtgcgg cgttgtattc aaacttctcc acccgcccgg caggggagtg
     2821 gcgggccagc aggtgatgga attcatccca ttcatacgtg gtggtgctgc cgtccggcgc
     2881 ttcttcccgc agaatattgc cgtcaggatc atagtaataa cggtgaatgc ccttgccgtc
     2941 cgtcacggtg gtgcagccgt caccatagtc cagcgtggcg ttgtaatagc cctccgcccc
     3001 ggtgacattc acgcaccggc cctgtgcatc gtaggtgaaa cggctccacg tctggtcgtt
     3061 atcggaccag cggatgatcc ggtccgcagc gtcgtactca taaaacaggt ggtagtccag
     3121 ccgcgcatcc gcttctgtca gccgcccccg cgcatcctgt tcgtaggtcg ccagcgtctg
     3181 tatgccgtta tccgtgcggt gaatggcttt caggtagccg ctggcatggt acgtcagttt
     3241 aagccggatc ccgtcggtgt gactgacacc cgtgatccgg tactggctgt cgcgctcaaa
     3301 cgtgatgcgg ttgctggcgg tgtcactcat cccgcccagc atatacatcc ggtcaccggg
     3361 gctgtgtgcc aggcggtcat cgctggtatc gtcagtcacc gggtggcgca gacgcattcc
     3421 cggaaaggcg tcataaaaga agcggctgct cagggcatca cggtcgaaca gctccaggta
     3481 atcatcacgg cggcgcagga tgtactgggc gtgatacggg ttaaccgccg tatcactttc
     3541 accgtcgaag gcgaagttca gcgtggctcc caggctgatg atatccaccc ggtttccctg
     3601 ttcacgcacc caggcgtatt cgctccagga gtcgctccag cccacgccgg tcaggccatg
     3661 cagcggcagt acagaactgt ggaaacgggt gaaggagagg gggagtgtct ggcccagggt
     3721 gaagtcggta cgttcgtcgc agtacgcgcc ggttaccgga tcgacaggat ctgttaccca
     3781 tctgatgact ttattggcaa tactttttaa cgacttacct gttttcccca gcaacgcgcc
     3841 gagctttgcc cccagcagct tcccgctttt ccccagcagg ttccccagtt tgccgagtct
     3901 cgccagattc ttcagttttc ccagcagtcc cgctggcggc agaagatagc tggctatcgt
     3961 tattcccccc aggatcattc tctgccacgg cgggaactcc ggctggatat ccagataggt
     4021 cacctgctca ccgccaataa agacgtccgg cgaacctccc acgattgctg cgctgcactc
     4081 cagtttgtcg ccctggcgtg cggcaggctg attgttgata aagacgttgc tgctgccctg
     4141 ggctatcctc ggtggtttgt tctccttgtc gcagagcgcc gtgtcctcac cggcttccgg
     4201 gaaatccaca tcttttttga tttcgccacg cccgcccacc atggtggcaa actccattaa
     4261 taccggaccg gcgggaaaca gaagcgatgc gccggcgctg acgcgatcca taaaaccggc
     4321 attgctgtcc gtcagggtga taacagcgtt cccccaggcc ttcgccagtg gtactgcttt
     4381 ttccgctgcc gattcaagca ggtcagtggc aatatcaccc catgagggtt ccccctgtgg
     4441 gacttcaggt attgctccag gtgggggagg agggggaagg gtgacggcgg ccctggcagc
     4501 gggctttccg ttaattttta cgttttcaga accggtattg atggcgccgt ccgtgtcaca
     4561 aaaactgttt atccatgact caagctggtt ctgtttctcc tccacataat cactcgcctt
     4621 atagctggca tacatcagcg caggcaccag aaccagcgca taagggccgg tggcacaggc
     4681 ggccataatc aggcaacctt ctgcgatggt cactatcgcc cccagcacgg cccctgccag
     4741 cgcccccaaa aaactcttgt gctgaatttc atcgcccagc ctggcggcgg gggggccaga
     4801 cttttttgat gccagcatct gtgcaatggc tttgtcgttg gctgcccgtt tcatctggct
     4861 ggcgaccatc gcctgctgtg acagcattcg cattgccagc gtaccgcctg cggtcgctcc
     4921 ctgtggcccg cctggtcctt cactcatgtg ttatccctcg tttatgtcag cgcggctggt
     4981 ccttttggat gtcattctcc ggtggcaacg gggtgaagct ctttatcacc tccagcagca
     5041 tcgtcttttg actgttcttc agggataagg aggtgctgaa agtgaaggtc agcagcgtat
     5101 ctccacgccg ctggaatacg gctatctggc taatccgccc ctgattcccc tcatcttccg
     5161 gaccgttcca ggaataatcc agcagccagg ccggggcctc tgccagagtg atttcccggc
     5221 aggcatgttc ggtgtaccca tcaagtttgc cctggtacag cgccagcgtt tcctggtaca
     5281 gctctggtgt cagggttccc tgtggaacgg cttcgcgggt aatgaccagg ttgatgccac
     5341 tgttatctgt ggacaccagg acctgcattg actgatcctg ccattgagac ggcagttgaa
     5401 tgtggccttc attaaaagtg aactgcataa aatactccct gataatctga gtgaatcagt
     5461 tgatgaaaac agaaccgccc acgatggtga tgccatcctt cgacagcctg attaccgtcg
     5521 attccccgac ctgtagctga atgccatccg gcgtcagcgc aattttgctt tcctccacgg
     5581 ccagcaaaat ttccttaatc gccgtataag cctgtatacc gtcgccttcg ccaccattgt
     5641 ttacagcgtc aacatgcacc gaaccggcaa tcgtgcctct gtcctgaaag acatcctcag
     5701 ataaagaccc tttcagtact ccggtgatct tgtggccttt ttccaccgta agaaggcggt
     5761 taccttcctg gatcgtgacc gtctggtcat tttttacggt ttcgcggtga tcgtgtttta
     5821 cggtggtggt acggtcgttg agcacttcgg tatccatgtt cttctgcgcg tggatgtaga
     5881 cctgttctct ccctgtcgca tcatcaaact tcagctcatt aaatccgctg cccatgtacg
     5941 ttttggagcg gatggtcatc tgcgtctttg ttcccggcag gctgccgggg gtgcggtttt
     6001 cctggtggta ggtgcgcccc ataatgatcg gctggtccgg atcgccgttg aggaagtcta
     6061 caatcacctc ctgacccaca cgcggtatcg ccaggtggcc aaaaccggtg cctgcccacg
     6121 cctgtgccac acggatccag cacgaactgt cttggtctgc cgggttataa cggtcccagt
     6181 tgaacttcac ccgcacgcga ccatgttcgt cgcagaagat ttcctcgcct gccggtcctg
     6241 tcacgacagc gctctgcggg ccgtcgacca gcggtttcag caacggctgt ggtcgccatg
     6301 ttctgtctgc cgggatcacc gcaaaatggt tctccagcgc cgtccccgct ccctgccgtc
     6361 ctggcaccgc ctgtggctgt tcgccgtgca gttcacttgc caccacctgc cattcccggt
     6421 tcaggttcgc ctgcggatgc cccgtcagca caattcgccg tcccggccat atctccggcg
     6481 aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca gccgtccatc tgccagcggg
     6541 caaagttctg cccgtgggca cccttgaaac gtccggggta gtcatacact tcatactgcg
     6601 tgcgctggta atcctggtac tggccttcct gatcaaaacg ccctggccag ccgggtcgtt
     6661 taaaggtgta gtccttggtc accacggaag aagggcggat ttgtgcgctg tagcggaact
     6721 ggctgatgca gagggtgctc acctcggtac gggtgttcgg gttccatggg atttcaaaag
     6781 attcgggcag atggcggact gtgtcgcaca gcaccaggct ctggtcggta cttttgtaag
     6841 catgctcctc ataaaagaag atgccttcct ccgccgccat ccggcacagg aaatcgtaat
     6901 cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg aacagcggac
     6961 tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg atgtcctcgt
     7021 tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg tgcaccttca
     7081 tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc accacgcctt
     7141 tcacccggcg ctgtacttca tcgccctgcc atatcgtcag gtaggccatt ttgtccagca
     7201 cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg agggaaaaga
     7261 gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcatcc ggcggcaggc
     7321 cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccggt aaaatgaata
     7381 atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca gacgttatta
     7441 gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt actttacaga
     7501 aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt tcttggcgtt
     7561 attcaggaca ggtacgcata tcacaacgag taacaaaaac g
//
LOCUS       DAMZII010000190         7423 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZII010000190 DAMZII010000000
VERSION     DAMZII010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.190"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..245
                     /gene="yafQ"
                     /locus_tag="POA77_004406"
     CDS             <1..245
                     /gene="yafQ"
                     /locus_tag="POA77_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDI6367739.1"
                     /translation="SKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSW
                     KGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            401..1141
                     /locus_tag="POA77_004407"
     CDS             401..1141
                     /locus_tag="POA77_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDI6367740.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1112..1879)
                     /locus_tag="POA77_004408"
     CDS             complement(1112..1879)
                     /locus_tag="POA77_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDI6367741.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="POA77_004409"
     CDS             complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="POA77_004409"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDI6367742.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRSAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2903..5347
                     /gene="fadE"
                     /locus_tag="POA77_004410"
     CDS             2903..5347
                     /gene="fadE"
                     /locus_tag="POA77_004410"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDI6367743.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLAGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="POA77_004411"
     CDS             complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="POA77_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDI6367744.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6017..6787
                     /gene="yafV"
                     /locus_tag="POA77_004412"
     CDS             6017..6787
                     /gene="yafV"
                     /locus_tag="POA77_004412"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDI6367745.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(6829..>7423)
                     /locus_tag="POA77_004413"
     CDS             complement(6829..>7423)
                     /locus_tag="POA77_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI6367746.1"
                     /translation="EKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAM
                     SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRY
                     YISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAI
                     NILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1726 a         1879 c         1959 g         1859 t
ORIGIN      
        1 tttcaaagga tgtaaaagtt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc
       61 ttatgacgct tcttatcaat aatactttac cgcttccagc tgtttataaa gaccacccgc
      121 tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt
      181 acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg
      241 ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc
      301 ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat ctattttgcc
      361 gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat
      421 tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc
      481 gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca
      541 gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcagct
      601 gctcgacagt tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg
      661 cgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag
      721 ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta
      781 tgaagggaaa tacctgatga tccacggcga ttgtgtttcc atcggctgct acgcaatgac
      841 caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc
      901 gagcgtgcaa gtgagtattt acccgttccg catgaccgac gccaatatga agcgccataa
      961 atattccaac tttaaggact tctgggagca actgaagccg ggttacgact actttgagca
     1021 aacccgtaag ccaccgaccg tttctgtggt caatggtcgt tacgtggtca gcaagccgtt
     1081 aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata
     1141 agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg
     1201 caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct
     1261 gatccagcag cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg
     1321 agcaatacgc catcacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc
     1381 gcagttcatc cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc
     1441 gcgggtaacg ctgcgttaat ttatgcagga gccagcagaa ggctttttcg ctatcggttt
     1501 caccgaccgg gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccgttat
     1561 gggcataagt ccagttacgc ccccataact cgcgggtaaa tgggtgagta ttttccagcg
     1621 ccacctcgcc ccgattagcc tggcgaatat gagccaccac cgaacaggat tttatcgggt
     1681 agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct ttaaatgtgc
     1741 gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac
     1801 caccacgctg tacaagtccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca
     1861 tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga
     1921 agattgattt aagaattttc aggtcggatg cggcgtaaac gaattattcg gcctacggtt
     1981 cgggtacaga tttgtaggca tgataagacg cgtcagcatc gcatcaggca tcagcgcaca
     2041 agtgccggat gcggcgtaaa cgccttatcc ggcctacgcc agacttactt aaccatctct
     2101 ttttcaatta actggatcag gatatggatc actttaatgt gaatctcctg aatgcggtcg
     2161 gcataaccaa agtgcggtac gcgaatttcg atatccgccg tgccagccat tttgccgccg
     2221 tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgctgc gatcgctttg
     2281 atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc ttcgcgacct
     2341 accgcttcaa cgtagcggga gaaaatatca ttgaaaccga aatcattacc gacgcaggaa
     2401 atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc acggtagcga
     2461 ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa
     2521 agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgagcg ctgaatggcg
     2581 tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc gttcagttcg
     2641 ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat agacaaaatg
     2701 cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac caggactttt
     2761 tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt atattgtaaa
     2821 cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg tcagacctcc
     2881 tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct
     2941 cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt tgctcgcctg
     3001 gacagccgcc ctcggcgttg ctggtctgtg gtcggcgtgg gtactggtgc ctctggccat
     3061 tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt
     3121 tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag cgattgatgc
     3181 gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga aaaagctgca
     3241 taactatccg cagccgcgcc tgaccgccga agagcaagcg tttctcgccg gcccggtaga
     3301 agaagcctgc cggatggcga atgatttcca gattactcat gagctggcgg atctgccgcc
     3361 ggagttgtgg gcgtacctta aagagcatcg cttcttcgcg atgattatca aaaaagagta
     3421 cggcggactg gagttctcag cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt
     3481 gagcgggatc ctggctatta ccgtcggcgt gccgaactca ttaggcccgg gcgagctgct
     3541 gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca
     3601 ggagatccct tgctttgcac tgacaagccc ggaagcgggt tccgatgcgg gcgcgattcc
     3661 ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg ggatgcgtct
     3721 gacctggaac aaacgctata ttacgctggc accgattgcg actgtgcttg ggctggcgtt
     3781 taaactctcc gacccggaaa agctgctggg tggcgcagaa gatttaggta ttacctgtgc
     3841 gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt
     3901 accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg attacatcat
     3961 cggggggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg
     4021 ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag cgctggcaac
     4081 gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga tggaagggat
     4141 tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg cggcatcgct
     4201 gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta
     4261 tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca ctggtggcaa
     4321 aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct taccagggcg caccgattgc
     4381 catcaccgtt gaaggggcta acattctgac ccgcagcatg atgatcttcg gacaaggagc
     4441 gattcgttgc catccgtacg tgctggaaga gatggaagcg gcgaagaaca atgacgtcaa
     4501 cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag
     4561 cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg atgccactaa
     4621 acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt ctgatgtctc
     4681 gatggcagtg ctgggcggca gcctgaaacg tcgcgagcgc atctcggccc gtctggggga
     4741 tattttaagc cagctctacc tcgcctctgc cgtgctgaag cgttatgacg acgaaggccg
     4801 taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt atcaggctga
     4861 acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa
     4921 tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc tggatcataa
     4981 agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc gcggtcagta
     5041 cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg tggatgtgat
     5101 tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc tgccgtttac
     5161 ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata aagatgaagc
     5221 cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg atgactttga
     5281 tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga aagttgaagc
     5341 cgcgtaaatg aataacggag ccgaaaggct ccgtttcttt atccgctaat tatttaaaat
     5401 taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacaac accgttttac
     5461 catcaatcga aagcgcatcg ttcacattca gccaggtgag tttctcttgc gacgttttct
     5521 catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac atcacagcga
     5581 tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac gtctcatctc
     5641 ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag gcaggcagct
     5701 tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt gcaaggctgc
     5761 taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg gcgactgtcg
     5821 ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta accttctaaa
     5881 gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca tttgttatca
     5941 atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa cccttcgcat
     6001 aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac cactggtgtg
     6061 gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg
     6121 cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag cggcagcttc
     6181 gtcgctagca caagatgacg tagtgaactg gatgacagct aaggcgcagc agtgcaatgc
     6241 gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc gctttttgct
     6301 ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc gcatggcaga
     6361 tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc gcggctggcg
     6421 tattttgccg ttggtgtgct acgacttacg ttttccggtg tggtcgcgca atctcaacga
     6481 ttatgacctc gccatctacg ttgccaactg gcctgctccg cgctctctgc actggcaggc
     6541 attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg
     6601 cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaacc cgcagggcga
     6661 gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgcggagc tgtcgatggt
     6721 ggcattgcgg gaatatagag aaaagtttcc ggcatggcag gatgcggatg agtttaggtt
     6781 gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg gggtaagact acgaaagccc
     6841 gctctccgca aggactgacg ccagatagtt tctgtccata gctgcttttc gcatcttacg
     6901 tcttaaccct gccttgaata ccttatcatt cgtcaaaata ttaatagcga tgtgccgtat
     6961 ccctgaaaat aattctgcgg cgtttcctct tcttattttg cagtcgtctt cattcattac
     7021 cacgtccaga cgccagtgca gcttattctc cacgtgccag tggtttcgga ttgctgtggc
     7081 gaacttttct gcggttaaat cagcagaact gatataatat ctgaccgtca tttctggctc
     7141 tttcttttgt tctgctatta ttgaccgaaa ggagactgcc acgcataatt tcttcagtcc
     7201 tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaacaa tatgaagacg
     7261 gatttcttct ctgccgtgac tcttttcact cattgcgtaa ctgtcatgct ctggattatt
     7321 taattctttc agcggaaatt tttcctcaaa ggctttattt agccgcccct ggtttccttt
     7381 taccgcgaat aaataatcac ctccctgttt ttgtatcttc tct
//
LOCUS       DAMZII010000191         7402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZII010000191 DAMZII010000000
VERSION     DAMZII010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.191"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(51..875)
                     /locus_tag="POA77_004414"
     CDS             complement(51..875)
                     /locus_tag="POA77_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDI6367747.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(928..2646)
                     /gene="proS"
                     /locus_tag="POA77_004415"
     CDS             complement(928..2646)
                     /gene="proS"
                     /locus_tag="POA77_004415"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6367748.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2757..3464)
                     /gene="tsaA"
                     /locus_tag="POA77_004416"
     CDS             complement(2757..3464)
                     /gene="tsaA"
                     /locus_tag="POA77_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDI6367749.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3461..3865)
                     /gene="rcsF"
                     /locus_tag="POA77_004417"
     CDS             complement(3461..3865)
                     /gene="rcsF"
                     /locus_tag="POA77_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDI6367750.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3983..4798)
                     /gene="metQ"
                     /locus_tag="POA77_004418"
     CDS             complement(3983..4798)
                     /gene="metQ"
                     /locus_tag="POA77_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDI6367751.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4838..5491)
                     /gene="metI"
                     /locus_tag="POA77_004419"
     CDS             complement(4838..5491)
                     /gene="metI"
                     /locus_tag="POA77_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDI6367752.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5484..6515)
                     /gene="metN"
                     /locus_tag="POA77_004420"
     CDS             complement(5484..6515)
                     /gene="metN"
                     /locus_tag="POA77_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDI6367753.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6703..7278
                     /gene="gmhB"
                     /locus_tag="POA77_004421"
     CDS             6703..7278
                     /gene="gmhB"
                     /locus_tag="POA77_004421"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDI6367754.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1801 a         1923 c         1894 g         1784 t
ORIGIN      
        1 gataaggcgt tcacgacgca tccggcattt tgtgcacgag gctggcttta tcactgagtc
       61 agcccaacaa agcgacccgc cttgatataa atcccaggct tcaggtgccc aacatactgt
      121 aactgcgttt ctggcttaaa cgcagagaca tcaccaatac gcatatgcat cagatcggca
      181 gggctaatcc agcccgattg cgccagtgta agcggatgcc cggctttggc aaatgcatca
      241 gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact gcttaactgc
      301 gctttcgcca ggccgctgac gcactgttgg cgaaaatcct cggagaacgg attcagtgag
      361 cacatctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata gttataaccg
      421 ctatctttga ttttattggc aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc
      481 gggactcgta agacgaaaag cttatcacta tgcttcatcg cttttttaag ggaaacgatc
      541 tggacgccag cgcctgtcgc ttctgcaacg ttattatcac ccagatagat tgcaacgtga
      601 ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag gcttgaggag
      661 aacagcaaat cgccgggttt gagatcaggt gccgttattt cttttattga ttgttcggtg
      721 aaagagcttt gatgctggaa tttgacagcc catgtttttg cctcgacatc taccgctgtg
      781 gcagaggagt ctggctggct gatatcaacg gtacaggccg aaagtaaaag aaagcagggg
      841 agaagcaggc ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg
      901 ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgac
      961 gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat attcgatatc
     1021 gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat
     1081 atcggcaaac atcacgcccg gacgttcttt tcggtcatcc agcagcactt cgataccttg
     1141 tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat
     1201 gttcatcggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg
     1261 ttcgtcgtag ttctgctcaa tcgccgcagc taccacacgc gttaccccga taccgtagca
     1321 acccatcgtc aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc
     1381 ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag
     1441 cgtaccctgg ccatccgggc ttggatcgcc cgccaccacg ttgcggatat cagcaatttc
     1501 cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat cgatgttagc
     1561 accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat
     1621 gtttaccgga cccagtgaac ccggaccggc gttaaccacg gcacgaattt cttcttcggt
     1681 cgcgaaagtc agcgggctgg caacctgcgg cagtttttct gctttaactt cgttcagttc
     1741 gtggtcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac
     1801 cagcagagtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat
     1861 ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc
     1921 tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa
     1981 gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg cgctgccgcc
     2041 gatagaaccg gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt
     2101 gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa
     2161 agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac
     2221 ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct
     2281 aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg
     2341 acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact
     2401 ctcttgccac aaatcggctg gctgaaccac cggcatcgac acctcgatcg caccggcgtt
     2461 gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag
     2521 ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg
     2581 gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt
     2641 acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa
     2701 aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa
     2761 cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa
     2821 tcatgtagcc agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgc
     2881 gggtcctgcg ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc
     2941 gtcaaaagct gcttttcgac ctctgcggta aaactcaccg ccatctctgc cgctggcgca
     3001 ctttgcgcat agctggcact ggcatcggga agcgattcgg caaaagggag atatggtttg
     3061 atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg
     3121 tctttatggc aaacaacctc tttcagctct accagcgaca taccaattgg gttagggcgg
     3181 aaagtagagc gtgttgcgaa aacccccatt ctggcgttac cgccgaggcg cggtggacgc
     3241 acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccacaaatgg
     3301 ctgaacgctt ccaggccgcg aacggcgtcg gcctggttgt agggagcaat gagatgcagt
     3361 tcgccgttgg cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag
     3421 ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa
     3481 gcgcagaacc gatacataca gcctgacgat agcagcctgg cgtaccgctg gtgacttcgc
     3541 agctatgcag taataccgca ttggccttca ttttcgaggc gttgatttgc atccgcttac
     3601 gtgcggttgg aatgctcggc ggagagtcct gattggaggc ctggcaagaa tcgccactga
     3661 cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga
     3721 ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc
     3781 tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta
     3841 ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat
     3901 tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca
     3961 aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt
     4021 ttgctgcttc gtaaacttca tcagactgat aagcctggac gaatttcttc acgttctcgg
     4081 cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggagtct ttatcttcga
     4141 caaagatacc gtctttcgct ggagtcaggc caatctggct ggcataggtg gtattgataa
     4201 ctgccagagc gatttgcgcg tcgtccagag atcgaggcag ctgcggtgct tccagctcaa
     4261 caattttcag attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc
     4321 catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaagattag
     4381 ttggatcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt
     4441 tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc
     4501 cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat
     4561 cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt
     4621 ccaggccata tttgtctttc gcaactttct gcgcgacttc tgctacctgc tgttcggcac
     4681 caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca
     4741 gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac
     4801 cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga
     4861 cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat accagtaccg
     4921 tattcatcac cgtcgcgtta tagccgatgt agccatactg atagccaatc tgacctaaac
     4981 caccggcacc gactgcacca cccatcgcgg aatagccgac cagggtaatc agggtgatag
     5041 ttgccgcatt caccagaccc ggcagtgctt ccggtaacag caccttacgg acgatctgca
     5101 tcggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag
     5161 cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga acaatcgctg
     5221 cctgcaaacc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca
     5281 agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct
     5341 tcgcgttagc aataatttgc cccgggcgcg taacataaag caggacgccg accggcagac
     5401 caatcacaaa gccaaaaaat ccggatacga aggtcattgc cagcgtttcc catacgccac
     5461 gaaccagcag ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt
     5521 tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc
     5581 atgatgccga acttaacgcc accggcgtag tccatctgcg cgctaataat gttgttgttg
     5641 acgttgaaac gacgcgcggt ttcagaaagc agtggggcat cgaccgattg accggtaaac
     5701 tccagacgca acatcggcac gcagtcagta aatggctccg cttgcagacg ttcctgataa
     5761 tcttccggga tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc
     5821 ggatgcgaaa acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc
     5881 gccacgcaat cacaaatgcg ctttacaacg tccatttcgt gggtgatcaa cagaatcgtc
     5941 aaacccagac ggcggttgat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg
     6001 tccagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg
     6061 gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta
     6121 tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt
     6181 gtgttgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg
     6241 ttaaaatgct ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac
     6301 agcgtggtca gttcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcagg
     6361 tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt
     6421 tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg
     6481 tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt
     6541 tagtcgtggc atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg
     6601 catccagacg tctaaatcaa tcgggtttat gcgaagagca ctttcttgca ggtcgaaaca
     6661 tgcgatacta gcgtcacatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc
     6721 gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc
     6781 gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc
     6841 tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt tactgaagca
     6901 cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgcga tgtcgatctg
     6961 gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc
     7021 tgcgactgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt
     7081 gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggctgcg
     7141 gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctattacgcc tgaagcagaa
     7201 aacgcggcgg attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag
     7261 caaaaaccgg cgcaatgatt aaaagatgag cggttgaaat aaaaatgcat ttttccgctt
     7321 gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt
     7381 cacacaaaca gccggttcgg tt
//
LOCUS       DAMZII010000192         7301 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZII010000192 DAMZII010000000
VERSION     DAMZII010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.192"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            221..429
                     /locus_tag="POA77_004422"
                     /pseudo
     CDS             221..429
                     /locus_tag="POA77_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            861..1424
                     /gene="fimA"
                     /locus_tag="POA77_004423"
     CDS             861..1424
                     /gene="fimA"
                     /locus_tag="POA77_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6367755.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1785..2495
                     /locus_tag="POA77_004424"
     CDS             1785..2495
                     /locus_tag="POA77_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6367756.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            2537..5188
                     /locus_tag="POA77_004425"
     CDS             2537..5188
                     /locus_tag="POA77_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6367757.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIVRGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            5202..5732
                     /locus_tag="POA77_004426"
     CDS             5202..5732
                     /locus_tag="POA77_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6367758.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRLVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            5745..6248
                     /locus_tag="POA77_004427"
     CDS             5745..6248
                     /locus_tag="POA77_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6367759.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            6307..7221
                     /locus_tag="POA77_004428"
     CDS             6307..7221
                     /locus_tag="POA77_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367760.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         2092 a         1547 c         1613 g         2049 t
ORIGIN      
        1 tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg ttattcgttc
       61 agggttcaag aacttaagcc tctcctcttt cataattggc ctgtgcatgg agaaatagcc
      121 tggttattcc ctgccccatg ctaagagcgc agtgtctgtc gcatagacac tgcatacgaa
      181 aacaccagag ggtgctgtta cttatttaag gacggatcaa gcgggaatga gtccactccg
      241 gtatcagaaa tggctacgtc tcaatgaagt caggcgaccg atgctgaatg aacattacga
      301 tgtcaccaca gcagcctatg ctgtcggtta cgaaagctat cccatttcag tcgggaatat
      361 tcgcggatgt ttggagagtc acccaagaga gatattacca ggttaagaaa gtctgtcggt
      421 cagctttaat aaaccctgca cttatctgtt tagagcaatt cggtgttagt ttcagcaagc
      481 aaacattaac catagctaat gatttatagc catattaacc attggtagcc cttgaccttt
      541 cctgacactt cccttgttcg caataatatt ctgtttagtc acggtttata aataacatgc
      601 ataatgaaat cgtcatgtta tttagcgaca taaaatgttt ttgcaaatac tctcttcaac
      661 aagtgcacaa taaacttgtt gtgattaggt gtccttgtga tttaaagata ttatcgttct
      721 tcgtgaaatg taataaccct ggaaactata ttctcagggc aacaaccaat caagaatgat
      781 tcttgttttt aagcaataac agaatgactt aattgttaac tctctttatt cagctactta
      841 aatataaatt ttggagaatt atgaaactca aacatgttgg tatgattgtc gtttctgtgt
      901 tggcgatgtc gtctgctgcg gtaagcgcag ccgagggtga tgaatcagta acgaccactg
      961 ttaatggcgg tgttattcat tttaaaggtg aagtggtaaa tgccgcttgt gcaattgatt
     1021 cagaatcaat gaaccaaacg gttgagctgg gtcaggttcg ttcttctcgc ctggctaaag
     1081 cgggtgacct cagctccgcc gttggcttca atatcaagct gaatgattgt gataccaatg
     1141 tttccagtaa tgcagctgtt gcattcctgg gtactactgt caccagtaat gacgatacgt
     1201 tagcgctgca gagttcagcg gcaggctctg cccaaaatgt cggtattcaa attttggacc
     1261 gtacgggtga ggtattaata cttgatgggg ccacttttag tgctaaaacc gacttgattg
     1321 atggcacgaa tatactgcca ttccaggctc gttatattgc tctcgggcag tccgtagctg
     1381 gtactgcaaa cgctgatgcg accttcaaag ttcaatatct ataattcaat cttaaggtgg
     1441 ctatacgtca tctgaaattg aaacagggtg tgtgtggcat ggctacgcac acccgagcaa
     1501 tttaccaata ttcatctcat ttttaatgag aaatggtttg tatttggagc gctatgaaaa
     1561 atcacataat atctaaacta tcaataaaca acagtagtta aacttactgt tatttaaaat
     1621 atttattttg tagcaaggtt ttcctattaa aagagaacca acatttatta caaacgcatt
     1681 aagtctggaa acccggcgac tcgccaatgt attagcaaca ttaatcgatt gtctgatgta
     1741 acggttttcc cgcttatcct cttaacacat ggagaatgca tcagatgcaa actacgcgta
     1801 ccccctactc tatttccttt atggcgacag tgttactttt attgctcttt gcttgccata
     1861 gcaccgttgc caacgcagct gtcgcactgg gtgcgacgcg ggtaatttat ccggcaaacc
     1921 agaaacaggt tttattgcct gtcacgaata atgatcctgc cagtgtctat ctcattcagt
     1981 catggataga aaatgcaggt gaccaaaaag acacccagtt tgtcattacc ccaccgcttt
     2041 tttccatgca aggtaaaaaa gagaatacgc tgcggataat caacgctact aatcatcagt
     2101 taccaggcga ccgtgaaagc ctgttctggg tgaatgttaa agctattcct gctatggaaa
     2161 aagaccagaa gaatgagaat acattacagc tggcaattat tagccgcata aaaatgtttt
     2221 atcgccccac gaacctggca atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc
     2281 gcagcggtag caaactcacg cttattaatc caacgcccta tttcattacc gtaacaaata
     2341 tgaaagcagg aaatagcaat ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg
     2401 tggatatcac gcatgctgca accggggata tcagttttca gacgatcaat gattacggtg
     2461 cattaactcc gcgtattaag gcaaccatgc agtaagccag tgacctaatt aataataaac
     2521 tcaatcggaa ttaataatga cagcctttcg tgccgcattt aaggcatatc gtatgcatca
     2581 ggtgttaata atgcctcgct ttgcacggct taccattgcc cttggcttag caactgcggt
     2641 atttcccgtt gacgcagagt attattttaa tccacggttt ttaagcaatg atcttgctga
     2701 atctgtcgat ttatccgcct ttactaaggg acgcgaggca ccacctggta catatcgggt
     2761 cgacatttat ttgaacgacg aatttatgac tagtcgggat attactttta ttgcagatga
     2821 taataacgca gaactcatcc catgcctgag tacagatctt cttgtcagtc ttggtataaa
     2881 aaaaatagcg ttattagaca ataaagaaca ttcggcagaa aaacatgtgc cggacaacag
     2941 cgcatgcaca ccacttcagg atcgtttggc cgacgcatca actgagtttg atgttggtca
     3001 gcaacatttg tcactctccg ttccgcaaat ttatgttggc agaatggctc gcggctatgt
     3061 ttctcctgat ctgtgggaag aaggaataaa tgctgggctt ctaaactata gttttaatgg
     3121 taattctatt aataatcgta gtaaccataa tgcaggaaaa tccaactatg catatttgaa
     3181 tttacagagt ggcatcaaca ttggtagttg gcgactgcgc gataactcaa cgtggagtta
     3241 ttacagtggg agtagcaatt catctgacag caataaatgg cagcatatca atacgtcggc
     3301 tgaacgtgac attattccct tacgctcacg gttaacggta ggtgatagtt ataccgatgg
     3361 cgatattttt gatagtgtga actttcgtgg cctcaaaata aattcaacag aagcgatgtt
     3421 gcccgatagc caacatggtt tcgctccggt gattcatggt attgtccgtg gaaccgcaca
     3481 agtgagtgta aaacagaacg gatacgatgt ttatcagact actgttccac ccggcccttt
     3541 tactattgat gatatcaact ctgcggccaa tggtggtgat ctgcaagtaa ccataaaaga
     3601 ggcagacggc agtattcaga cattatatgt tccttattcg tctgttccgg ttctccaacg
     3661 tgctggatat acgcgttatg cgcttgccat gggggaatat cgtagtggaa ataacctgca
     3721 aagctctccc aagttcatac aaggtagctt gatgcatggt ctggaaggaa actggacacc
     3781 ttatggcgga atgcaaattg cagaagatta tcaggccttc aaccttggta ttgggaaaga
     3841 tttaggactt tttggtgcct tttctttcga tatcacgcag gccaatacga cacttgcaga
     3901 tggcacccgt cacagtgggc aatcggttaa atccgtctac agcaaatcct tctaccagac
     3961 aggaaccaat atccaggtcg caggatatcg ctattctacg caaggttttt ataacttatc
     4021 cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag cctcctaccg gagacaccaa
     4081 tgagcagaca caatttattg attattttaa tctgttctac agtaagcgtg gtcaggaaca
     4141 aataagcatc tctcagcagc ttggaaatta cggtacgaca tttttcagtg ccagtcgcca
     4201 aagttactgg aacacgtcac gcagcgacca gcaaatatca tttggattaa atgtgccgtt
     4261 tggtgatatt acgacttcgc tgaattacag ctattccaat aatatatggc aaaacgatcg
     4321 ggatcattta ctcgctttta cgcttaatgt tcccttcagt cattggatgc gtacagacag
     4381 tcagtcggca tttcgtaatt caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg
     4441 catgaccaat ctatcggggg tttatggcac tctgctgccg gataataacc tgaattatag
     4501 cgttcaggtc ggtaacaccc acggaggtaa tacatcgtct ggcactagtg gttacagttc
     4561 tcttaattat cgtggagctt atggtaatac taatgtcggt tacagtcgga gtggtgacag
     4621 cagccagatt tattacggaa tgagtggtgg gattattgct catgctgatg gcatcacctt
     4681 tggacagccg ctgggcgaca caatggttct ggttaaggct cctggtgctg ataatgtcaa
     4741 aatagagaac cagaccggaa ttcataccga ctggcgtggc tatgccatat taccatttgc
     4801 gacagaatat agagaaaacc gtgttgctct taacgcgaat tcccttgcag ataatgttga
     4861 actggatgaa accgtggtca ctgtcatccc aactcacggt gctattgcca gagcaacatt
     4921 taatgcacaa atcggcggga aagtattaat gacgttgaag tacggtaata agagcgttcc
     4981 attcggtgca attgtcacac acggagagaa taaaaatggc agcattgtcg cagaaaacgg
     5041 tcaggtttat ctgactggac ttccacagtc agggaaatta caggtttcat ggggcaaaga
     5101 taaaaactca aactgtattg tcgagtacaa gcttcctgaa gtttctcctg gtaccttact
     5161 gaaccagcag acagcaatct gtcgctaagg aacaatagat tatgaaatac aataacatta
     5221 ttttcctcgg tttatgtctg gggttaacca cctattctgc tttatccgca gatagcgtta
     5281 ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat tcgcttaatt
     5341 ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc aacgaccggt agcacaagtc
     5401 cagccgtccc ttttcagatt acgttaagtg aatgcagcaa agggacaacg ggggttcggg
     5461 ttgcatttaa cggtattgag gatgcagaaa ataatacttt gttgaaactg gatgaaggaa
     5521 gcaatacggc ctccggtttg ggtatagaaa tattggacgc aaatatgcgt ctggtgaaac
     5581 tgaatgacct tcatgccggg atgcagtgga tcccactggt accagaacag aacaatattt
     5641 tgccttactc cgctcgtctg aagtcaactc agaagtccgt caatccggga ctggtgaggg
     5701 cttcggcaac ctttaccctt gaatttcaat aatcagagtc agatatgaaa agacttcaca
     5761 agaggttcct gttagctacg ttttgcgcgt tattcacagc aactctccag gccgccgatg
     5821 tcactatcac tgttaatggt cgggtagtcg ctaaaccctg cactattcaa accaaagaag
     5881 ctaacgttaa tctcggggat ctttatacgc gcaatctgca acaacctggt tctgcatctg
     5941 gctggcacaa tattactttg tcattaaccg attgtccggt tgaaacaagt gcagtgacgg
     6001 caatcgtgac aggttcaact gacaatacgg gttattacaa aaatgaaggt actgccgaaa
     6061 atattcagat agagctgagg gatgaccagg atgcgacgtt aaaaaatggc gatagcaaaa
     6121 cggttattgt tgatgagatc actcgtaatg cacagtttcc acttaaggca agagctatca
     6181 cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg atctacacct
     6241 ggcaataacg tcgattcgat tttcttagcc ttctttttaa atcaattttt aaccttagga
     6301 tatatcatgg gtaaaacaat cagtataaag gtcctgttcg gtatttatct tctattaatg
     6361 gcaggcaaag tttttgcctt cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt
     6421 acaacctctg tttatgttaa tctcgaccct gtaatacagc cgggccagaa tctggttgta
     6481 gacttgtctc agcatataag ttgctggaat gattacggcg gctggtacga cactgatcat
     6541 ataaacctgg tacaaggctc agctttcgca ggctccctac agtcatataa aggatcgctg
     6601 tactggaata atgtgaccta ccccttcccg ttgacgacaa ataccaacgt attggatatt
     6661 ggtgataaga caccgatgcc attaccgtta aaactctata ttaccccagt tggggcggcc
     6721 ggtggagttg tcataaaagc gggggaagtc attgcccgta ttcatatgta taaaattgca
     6781 acattaggaa gcggaaatcc gcgtaatttt acgtggaata ttatttcaaa taacaatgtt
     6841 gttatgccaa caggcggctg caccgttgat agtcgtaatg tcaccgtgga tctcccggac
     6901 tttcctggaa gcgcagaaat accccttggc gtatattgct caagcgaaca aaagctctca
     6961 ttttatcttt cgggtgccac cactgacagt agccgacaag tgtttgctaa cacagcacct
     7021 gatgcaacaa aagcatcggg agtgggtgtc actctaatgc gtaatggaaa aatactggct
     7081 acgggagaaa atgtctccct ggggacagta aataaaagca aagtacctct gggtttaagc
     7141 gcaacctatg gacaaacagg aaacaaggtt tcagccggaa ccgtccagtc agtcattggt
     7201 gtcactttta tctatgaata attgtgattt tgaccttagt ctatgcgctc cattcacgaa
     7261 aattgcgcgg ataattattt tgtgaaggct attagcctac a
//
LOCUS       DAMZII010000193         7230 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZII010000193 DAMZII010000000
VERSION     DAMZII010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.193"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(291..1427)
                     /locus_tag="POA77_004429"
     CDS             complement(291..1427)
                     /locus_tag="POA77_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6367761.1"
                     /translation="MDLLIVLTYVALAWTVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1430..1792)
                     /locus_tag="POA77_004430"
     CDS             complement(1430..1792)
                     /locus_tag="POA77_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI6367762.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2329..4242
                     /gene="mtlA"
                     /locus_tag="POA77_004431"
     CDS             2329..4242
                     /gene="mtlA"
                     /locus_tag="POA77_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDI6367763.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMLFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4472..5620
                     /gene="mtlD"
                     /locus_tag="POA77_004432"
     CDS             4472..5620
                     /gene="mtlD"
                     /locus_tag="POA77_004432"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6367764.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5620..6207
                     /gene="mtlR"
                     /locus_tag="POA77_004433"
     CDS             5620..6207
                     /gene="mtlR"
                     /locus_tag="POA77_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDI6367765.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6219..6428)
                     /locus_tag="POA77_004434"
     CDS             complement(6219..6428)
                     /locus_tag="POA77_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367766.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6713..7075
                     /locus_tag="POA77_004435"
     CDS             6713..7075
                     /locus_tag="POA77_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDI6367767.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1827 a         1820 c         1841 g         1742 t
ORIGIN      
        1 tcatccttca gataataaag ccatcatcgc aatagatgga aaagcactcc tgctcttttt
       61 tgattagagc cgtcgcagga ggaacaactc atatcatcat tgcgatctcg acgatgtata
      121 gtctcatcct gcggcagaac aagacggata aaaaatccaa tgagagcaca ctcatccaga
      181 acttattaac ataatgaata ttaaagaaaa atcatcgcag ctaatgaagt aatatgcaaa
      241 aataattagt ccttacaagg agcaaagttc cataatctcc cccctccccc tcaatgatcc
      301 aaataaagat aatgcatcca gctggtcatt cttagcagca ctttccgcat caccgaaaca
      361 tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg taaggcatcg
      421 atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggcacgacc
      481 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaata
      541 ctagtcagtt tgccgtgaaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
      601 tcgccgggtt tcagacgtaa cagtgagttt tgccgaaatt gggcgacaat ttgccgtttt
      661 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
      721 cggatcagta cctgagtaac gtagccattg ctcggcgcgc gaatgacagt ctgctcaagg
      781 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgtta
      841 accatactgt cgagctggct ctggatctgc gcctgctccg ccaccgagcc tttcaccagc
      901 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
      961 accgccgcct ggctgccttt caagtaacgt tgataatttt taaacagacg gtcgcgctcc
     1021 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag cgtctttata
     1081 ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg gtaacgaacc
     1141 gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt gtcagtgact
     1201 tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc cttttgcgcg
     1261 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatta aaccactcac cagaaacacg
     1321 cctcccagcg ccgccgtcgc cagcgtccac tgatttaccg gaatgcggaa gattttaaag
     1381 accgtccacg ccagcgccac gtaagttaaa acaatcaata gatccataat tagatctccg
     1441 ggaacgtgga atagtcagtc tttttctcgg cggcgagttg atgctccagc cgggaaattc
     1501 ggtcagaaag tgaggcgatt tccgggtcag tcgctttctc ctgcgacgca acatgtgact
     1561 gcatacccca gccacgctcc ggttgataga gcgtcgccca gatccacaga aacggccaga
     1621 taacatgcag agtaaacagg ctcacccagc ccgccgtatg aatagcgtcg gcatgggggt
     1681 gattgcgctt tttggcaatc agataaggga tgtcatgtat cgcgatgatt ccataaaaaa
     1741 tcaccagaaa gacgaagatc agcactccca acgcgaaata gtttagaaac atatctgcct
     1801 cggattcacg tttatcagtg ttgtttttgg gctggcagcc agaagggagt caggctgata
     1861 ttttgacaat aatccgggtt cgcgattctc gccataacac caaagaataa tttttagagg
     1921 tgatgagttg cttagttaca taacgattgt atgacgaagg cataacatgc tgtagatcac
     1981 atcaggtgaa cgccgtaaga aaatatcttg tgattcagat cacaaagatt caacaaacca
     2041 tcaaaacaaa aatgtgacac tactcacatt taaatgccat ttttagcgaa aatcgccgtc
     2101 ttgttgcttt tttacacaag cgttttgtga tgagtatcac gtcaattacc tctctacccc
     2161 ctatatttat gtgactgata tcacacaaaa ggccgtcgac tggacagtta accgattcag
     2221 tgccagattt cgcagtatct acaaggtccg gctacctctg ccgccacatt aacaaaaaac
     2281 ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg gtgtttttat gtcatccgat
     2341 attaagatca aagtgcaaag ctttggtcgt ttcctcagca acatggtgat gccaaatatc
     2401 ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta ttccaacagg gtggttaccg
     2461 aacgagacgc tggcgaagct ggtcgggccg atgatcactt atctcctgcc gctgctgatc
     2521 ggttataccg gtggtaagct ggtaggcggc gaacgtggcg gcgtagtcgg tgccatcact
     2581 accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc tcggttctat gattgcaggt
     2641 ccgctgggcg gctggtgcat taagcacttc gaccgctggg tagacggtaa gatcaaatcc
     2701 ggttttgaga tgctggtgaa taacttctcc gcaggcatca tcgggatgat cctcgccatt
     2761 ctggcatttc tcggcattgg cccgattgtt gaagccctgt ccaaaatgct ggctgcgggc
     2821 gttaacttca tggttgtcca tgacatgctg ccgctggcgt ctatctttgt tgaaccggcg
     2881 aaaatcctgt tcctcaacaa cgccattaac cacggtatct tctcgccgct gggtattcag
     2941 cagtcccatg aactgggtaa atccatcttc ttcctgattg aagctaaccc aggcccaggt
     3001 atgggcgtgc tgctggcgta catgttgttt ggtcgtggta gtgctaaaca gtctgcgggc
     3061 ggtgcggcaa tcatccactt cctgggtggt atccacgaaa tctacttccc gtatgtgctg
     3121 atgaatccgc gtctgatcct ggccgttatc ctcggcggta tgactggcgt gttcacgctg
     3181 actatcctgg gcggtggtct ggtttctcca gcatctccgg ggtctatcct tgcggtactg
     3241 gcgatgacgc caaaaggtgc ttacttcgct aacatcgcgg gtgtgtgtgc ggcgatggct
     3301 gtctccttcg ttgtctctgc tattttgctg aaaaccagca aagtgaaaga agaagatgat
     3361 attgaagcag caactcgtcg tatgcaggac atgaaagctg agtctaaagg cgcatctccg
     3421 ctgtctgctg gcgatgtgac taacgacctg agccacgtac gtaaaatcat cgttgcctgt
     3481 gacgccggta tgggttccag tgcgatgggc gcaggcgttc tgcgtaagaa aattcaggat
     3541 gcaggtctgt cgcagatttc tgttactaac agcgcgatca acaacctgcc gccagatgtg
     3601 gacctcgtca tcactcaccg tgacctgacc gaacgcgcta tgcgccaggt tccgcaggca
     3661 cagcatattt cgctgaccaa cttcctcgac agcggcctgt acaccagcct gaccgaacgt
     3721 ctggttgctg cccaacgcca cacggcaaac gaagagaaag taaaagacag cctgaaagac
     3781 agctttgacg attccagtgc taacctgttc aagctaggcg cggagaacat cttcctcggt
     3841 cgcaaagcgg caaccaaaga agaagcgatt cgttttgctg gcgagcagct ggtgaaaggc
     3901 ggttacgttg agccggaata cgttcaggcg atgctggatc gtgaaaaact gaccccgact
     3961 tatctgggtg agtctatcgc ggtgccacac ggtacggttg aagcgaaaga tcgcgtactg
     4021 aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc gcttcggtga agaagaagat
     4081 gacattgccc gtctggtgat tggtattgca gcccgtaaca acgagcacat tcaggttatc
     4141 accagcctga ccaatgcact ggatgatgag tccgtcatcg agcgtctggc acacaccacc
     4201 agcgtggatg aagtgctgga actgttggca ggtcgtaagt aatccaatcc caccctctcc
     4261 acatggagaa ggtggggtta attgcctgat gcgctacgct tatcaggcct acaggatgca
     4321 tcacgatttg ttgaatttgc acgttcttgt aggccggata aggcgcttac gccgcatccg
     4381 gcgctgcccc tctcctcacg gagagggttt gggtgaggga aaagcctcac cccagccctc
     4441 tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg gcgcaggtaa
     4501 tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac tgacgtttgc
     4561 cgatgtcaat caggtggtac ttgatgccct gaatgcccgt catagctatc aggttcatgt
     4621 ggttggtgaa accgagcagg tagataccgt ttccggcgtc aatgctgtca gcagcattgg
     4681 tgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg ttggcccggt
     4741 tgtgctggaa cgtattgctc cggctatcgc caaagggctg gtgaaacgca aagaacaagg
     4801 taatgaatcc ccgttgaaca tcatcgcctg tgaaaacatg gtacgcggta ccacgcagct
     4861 gaaaggccat gtgatgaacg ccctgccgga agacgccaaa gcgtgggtag aagaacacgt
     4921 tggctttgtc gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg caactaacga
     4981 tccgctggaa gtgacggtag aaactttcag cgaatggatt gtcgataaaa cgcagttcaa
     5041 aggcgcactg ccgaacatcc caggcatgga gttaaccgac aacctgatgg catttgtcga
     5101 acgtaaactc ttcaccctga acacgggtca tgctataacc gcgtacctcg gaaaactggc
     5161 cggtcatcag accattcgtg acgctattct cgacgagaaa atccgcgcgg tggtaaaagg
     5221 tgcgatggaa gaaagtggtg cggtactgat caagcgctac ggctttgacg cagacaaaca
     5281 tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac ccgtatctga aagatgatgt
     5341 agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc tgatcaagcc
     5401 attgctcggt acgctggaat acagccttcc gcacaagaat ctgattcagg ggattgctgg
     5461 tgcaatgcac ttccgcagtg aagatgatcc gcaggctcag gaactggcag cactgatcgc
     5521 tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt cttgatgcca acagcgaggt
     5581 tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca ggcgcaggac
     5641 accctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca aacccaggcc
     5701 tttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc
     5761 accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt attccgcaaa
     5821 gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgacgg tccgctgggc
     5881 gatctttctg tgcgtttaaa actcatttac gggctgggcg tcattaaccg ccaggaatac
     5941 gaagatgcgg agctgctgat ggcattgcgt gaagaactaa atcacgacgg caacgagtac
     6001 gcctttaccg acgacgagat ccttggaccc tttggtgaac tgcactgcgt ggcggcgtta
     6061 ccaccgccgc cacagtttga accagcagac tccagtttgt atgcaatgca aattcagcgc
     6121 tatcaacagg ctgtgcgatc aacaatggtc ctttcactga ctgagctgat ttccagaatc
     6181 agcttaaaaa aagcctttca aaagtaagca acgtctgctt actgcccctc tacctgcttc
     6241 ggccgataaa gccgacgata atactccaga cgttgtagat ataacggcac gctttcatcg
     6301 ggtattccgg acggaatcgc gttacgggga ggaagttttt tcagatactc ccggaacgcc
     6361 tggcttgatg ccatgaaatc tacggcttta tcgataagaa gcggaacgtt ttcacctaat
     6421 ttgcccatga tgatatctcc gtattacccc gcgccgggaa tgcgcggccg ccaattttag
     6481 tttaggttcc cggtaaagat gacattaagg aaaacgtgct gaatcctcaa taatcatccg
     6541 ctgctgttta ttatcttttt tctatagatt tcttaattaa tcaataaatt gcgtagtttt
     6601 taagcatgtt aatgattacg ttacagaact attgcaatgt catcagttgt gaacaaagca
     6661 cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc acatgaaaga
     6721 agtcgaaaaa aacgaaatca aacgtctcag cgatcgtctg gacgccatcc gccaccagca
     6781 ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa aagagaaagc
     6841 cacgctggaa gcagaaattg ctcgcctgcg tgaagttcat agccaaaaac tgagtaaaga
     6901 agcacagaag ctgatgaaga tgccgtttca gcgcgcaatt accaaaaaag agcaggctga
     6961 tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct
     7021 gggccgtgaa atgggcctgc aggagatgac tggattctct aagactgcgt tttaagcgcc
     7081 tcagacgact ggttcgatca tatcaacccg catctggctt gccttagctg gtcaatgcgg
     7141 ggactctcct gtctactcgc ctaattacca aacaattcac tccctttact tcgatttacc
     7201 gctggttgat attctgaaaa tgcattttta
//
LOCUS       DAMZII010000194         7202 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZII010000194 DAMZII010000000
VERSION     DAMZII010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.194"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(521..793)
                     /locus_tag="POA77_004436"
     CDS             complement(521..793)
                     /locus_tag="POA77_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI6367768.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNR"
     gene            complement(1076..3763)
                     /locus_tag="POA77_004437"
     CDS             complement(1076..3763)
                     /locus_tag="POA77_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6367769.1"
                     /translation="MKTQSVSTISGAATGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEAAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKGLHGYSLPLLSQPLNLAGITFSSGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSADWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKNLAEHLEQHVAH
                     YHGAPFRDWLHCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQRCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(3760..4170)
                     /locus_tag="POA77_004438"
     CDS             complement(3760..4170)
                     /locus_tag="POA77_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367770.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMERVHNEPVYIEDGER
                     IELTHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(4163..4399)
                     /locus_tag="POA77_004439"
     CDS             complement(4163..4399)
                     /locus_tag="POA77_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367771.1"
                     /translation="MKSMNFKQGCILSELGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGEMRDE"
     gene            complement(4396..4986)
                     /locus_tag="POA77_004440"
     CDS             complement(4396..4986)
                     /locus_tag="POA77_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDI6367772.1"
                     /translation="MPALINPGNVQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCNL
                     TATQHAPGVFFCVVALTHLKFMVWWLTACFYFLRHIIRIMVVQAGQLSGWPVSVRAGI
                     PTPVWATTHERRNSGGSVTRYLTEVAIMATVPALSHPEFTFVFLAVRRTDRDACPRPV
                     RVIADCEHAARLKLATEFILSFAARIPVKSAGEVVA"
     gene            complement(5074..6078)
                     /locus_tag="POA77_004441"
     CDS             complement(5074..6078)
                     /locus_tag="POA77_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDI6367773.1"
                     /translation="MYAFKTERPDAARRYHNKFVTGTNYQEESNRRFELSEPTENILR
                     TSILESGWLIKNITLRDVNAVSGNAVNIGASELHTGRVDGGRFHKKMAINGTEFFLTE
                     TDTCARISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEI
                     NKKGEDVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDSLANHLITELIAEQFRE
                     DPRLVVLVGAELAAHQRLKLFNAADRPADVNAAQMATSSVAGRFAFIPPFMPGKRLAV
                     TTLDNLHIYTQAITRWFRAEFDDENSEYVHSYLRNEGYALGEPELYAAVDESALTFAD
                     "
     gene            complement(6101..6961)
                     /locus_tag="POA77_004442"
     CDS             complement(6101..6961)
                     /locus_tag="POA77_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367774.1"
                     /translation="MIDNVFKKKLASIKNEHVSVLDSYKVSPFKETHSDIACIVRIIE
                     IYSLNKLRAIGEKLYSLTGLTVPDTEAVANEINLLLTRYAQLCRQEEEELSFRQREVT
                     NAEVAWKSTSSKNGVSSIAEAKTNKTGHAERADAERCYHLAVSRLNEQHSRLSTIKLL
                     PGVLADEVNYIGKGVEKRLLNIFPQSGQIPADFISVFNDGDVVRDIKFITDALKSLSD
                     SVSEIISRCSVPTDRYVLNNGGMARAMAYREYYRADNYVLRSVVSDRDYVEHVMKYNR
                     VTEYKNKIFS"
     gene            complement(6979..7197)
                     /locus_tag="POA77_004443"
     CDS             complement(6979..7197)
                     /locus_tag="POA77_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6367775.1"
                     /translation="MPRENAPQKNYRLLRISEVINLTGLPKSTIYLKIKNDEFPNQVS
                     IGSRSVAWVEHEVNEWIEKNILNRKLNS"
BASE COUNT         1818 a         1938 c         1511 g         1935 t
ORIGIN      
        1 tctagacatc aaaaatatcg tcaccaagac atatcaacca attgatttct atgaaaattc
       61 atttactttc agaaaatttt tctttgatat caacactatg aaataatgtt cttttcataa
      121 tcgtgcatag aactatggca tacacacgaa aaccattttt gacaaaaatc gcactacacc
      181 gcacccgcct gcggttttca tatcactaaa atttttcagt caggattttt tacaaacgac
      241 accgccagtc cgcgccggtg ctggggatgt actgcgatac aggaactgaa aagaatgaaa
      301 aaaaattcag attttttcag ttttactgtc cgttgtgtct gtggtctgaa tacgcaacac
      361 tatgatatta aatggataat ttattttagg tgtgtgattt tatgaaggct cgtaaaacgc
      421 gaaaaatata accatatgaa aaataataac tttatattct aattaactga aatcctgtgt
      481 tcaggtttcg ggaacggatc ggccattccc gacgcatact ttacctgttc ttttgcgcag
      541 ccagcagcaa ctttaaagcc ttttcccgct cctcgggtgg aatggcatca attagctctt
      601 taaccctccc ctgcccgttc agcgcgcttg gactaacgga atgggagaat gcaacattca
      661 tcacaaacga gtgaccgcac tccacattag agcaatagca gtacacatca tacagttgtg
      721 ggtgtttcct gtttgtttta cctatccgcg ctactgcctg acatgtgggg cagtacattc
      781 tcatcatctt catcgttctg tgccctatac tgttgcccgc cagaggaagg gatttgagtc
      841 cctacctctt gttctaaacg tgtaacactg gtaacacgtg taacgttatt gatttaaaag
      901 gacttaatcc gttaccagtt agcataatca agaggtaacg cgtggtaaca caccattttt
      961 tgttaccagt gttaccagtc agtatttttg actggtaaca ctgaagaacc gcatcagtag
     1021 caggcgttac ctctgttacc agtgttacct cataaaaata agactcacgc gataattact
     1081 ccggttcggc gcttcccagt acctgtgtat tgaactggta cacacgttta agtcctattt
     1141 ctggcaggcg aatgctgttt tgcgtccgac catcttcacc gggtttcagc cagcccgcgt
     1201 ccacacacaa cctggccacc ttacgcgaat caaatccctt acagatctcc ttccagccag
     1261 acgggagaac gtaaaacgtc acaaccggct ccgttacgtt gtctccttta tcgacttttc
     1321 tgaatcccat cattgaaaca ggccggttcc tgtcgtcatt ccagtcagca aaccgactga
     1381 actgattacg cgtcatgaag tcccgtactt gttcaagtgc cgctttatct tcctgattag
     1441 ctgtatggcc ccggtcagcc atccatgcag ccagacaacg ttgagcggca cgaaaggctt
     1501 ctccctccgg ccatcctgtg atgcccgctt ttgtggccag ttccccggcc atcgcaacca
     1561 gagcaaaacg ggtaacagcc cggccaacct gattcccggc attttccggc gtcagccttc
     1621 gggtgtactc tttcaataag gctttcgcct ggctggtcag ttccggcaga tcagcggtca
     1681 ggcaatgcag ccagtcgcgg aatggtgcgc catgataatg cgctacgtgc tgctcaagat
     1741 gctcagccag gtttttcccg ctactgaagc catgaagttc ttcaaacacg ccatatttgc
     1801 ccgaatcgct tgggatctgg atcattctga cctcaacccc ggcatacgta cgttccccgg
     1861 cgcttgccgc atgttccacc agtgacagtt ccccggtgga cagaaacaac agattccagc
     1921 ggttggtttc ccttaccgaa ccatctgttc tggctctggc tttgccctgc ccgttagcca
     1981 gcatataagc gatattcccc gcctccctgc cgtcaacctc ccggatttca tccagcatca
     2041 gcgtggcatc attgcggcgg ctcgccgttc cttctaaggc attccccgtc gcccgccacg
     2101 tatgccagaa atctgttcca ccacatactg atgcagccac tttcatcgtc gtggttttac
     2161 cgtctgttga ttcccctttg aggtgataac ctccaccgcc gataccaacc agtttcagaa
     2221 ggggggcagc aaatgccaga ctcaccgcaa aggccagacg ggcattcttt atgcagtaac
     2281 ggcctatatt ttcacgccag tccgctgatg tgccgctgac acgaaaatca cgcccctgca
     2341 cgctcgatgt ttgtaagatg actgactggg cttcgcgccc tatcacttca tcctggagaa
     2401 cgtaaacccc gccgtgccag cccgttttat tcacacaggt tacttttctg tccggtttac
     2461 acagcgagat atattccatc aggaatgctc tggccatgcc gttaatgttg atgtacgaaa
     2521 gcccgttaac cagcagaacg cggcgcagct cttccccgct gccacccagc atctccatcg
     2581 gcattgccca tttacggctg tttccgttgg tatcttccca ttccagcaaa cgcccgtaat
     2641 tactcccatc agcatcactg gtgattgccg tcacccgcaa cggactgcaa attttgatat
     2701 tacgtatctc cgtatcgcca tccgacttat taaccagctt gtcgtaccac aaatattctt
     2761 ttgtcagccg gaacccttct ggtaaacggg tctgcccttt gccatgcagc gtcatttctt
     2821 cacgaaaggc atctctcgcc cgttctttcc caacttcctg ccgataatca tcccagtcag
     2881 ccttatggcg taccggaggg agcgttaccc agccatccac cgctttggcg gctttctctg
     2941 cctgaatacg cccggtgttt tctttcccgt cagccagatc attatctccg gccagaatga
     3001 tccgggtttc cggccagcgt tttcggatct gctccgtcac cttcagtaaa ttggtggcag
     3061 cgacagccgc cacgatccat ccttcagtca gcagactgac ggtaagcgcc gtggcaaaac
     3121 cctcagtgat aatcacctgt tcaggtgttt cagcgggtat atcggtcagg gcaatgaatg
     3181 cgccagacag ctggctacct ggcagcagac ttttatcccc gtcaggattg ataagctgac
     3241 caccagtaat attcccggag atatccgtca acggcagcag taatgaaccg gaggaaaatg
     3301 ttattccggc cagattcaaa ggctgtgaca gcaggggaag tgaatagccg tgtaagccct
     3361 tgtttgtcag atagaccggc tcaccattgc aggactgctg tctgagtctg gtatatcgct
     3421 ccgcacccgc ttcttttcct gcggcctttt tcctggcggg caaagcgggc ttctcctgta
     3481 tttctggtaa tgcaagcgcc tcagatacca tcccggcggc ttcttttatc tttcttcctg
     3541 tcaccagacg aaccagatca agaccatcgc catgaccgca ctgattacaa aaccacgtcc
     3601 cacgccccgc tttatcgtcc agtcgaaagc gatctttgcc accacaggca gggcaagccc
     3661 catgatgtcc tgcggacggt actttgatac ctaatgctga caggatataa ggccagcgcc
     3721 cagtcgcagc cccggaaatc gtggatacag actgtgtttt cacagcagca cctcctgacc
     3781 ttccggcttc acaaattcaa aaccttcggg tagttcgcca tcaggtgcac cgaaaataat
     3841 atcccggtaa acctcacgca tctctgtgac accaaaaact gacaggcgaa tattttctga
     3901 cataaagcct ggtttaagca ttttttccag tttctctgcg gccagtatgc aaccagcatc
     3961 agccccaaac tcattcaccc acggcatttc aatatggtaa ataatattgc tcaccacttt
     4021 ttcgtgggtg agttctattc gttcgccatc ttcaatataa acaggttcat tatgaacacg
     4081 ctccattaag gaaatgaatg catcagcaat gtagtaccgt aagactgctg ttcgtaaagc
     4141 atgcgccttt aaattttgat tattattcat cgcgcatctc tcccttaatt gtgctggttt
     4201 ccttaacaac gccatccagt tgttcggcaa tacaggccac taaagccgca gcgccttccg
     4261 cggaaacagt tctcgctcca ttcactgtgg gaacggacag gacctctgca agaaactcac
     4321 tggccagtgc cgcccgtaac agacgactct cacccagttc gctcaatatg cagccttgct
     4381 taaaattcat ggatttcatg caaccacctc cccggcactt ttcacaggga tacgggcagc
     4441 aaagctcagg ataaattcag tggcaagttt aaggcgtgca gcatgctcgc agtctgcgat
     4501 tacacgcaca ggacgtggac aggcatcacg gtctgtacgg cgaacagcca gaaagacaaa
     4561 ggtaaattca gggtgagata aagcagggac tgtagccata atggcaacct ccgttagata
     4621 gcgggtaacg ctaccaccgg agttcctacg ctcatgggtg gtagcccaga cgggggtagg
     4681 aataccggct ctaacggata ccggccagcc cgaaagctgc cccgcctgga ctaccataat
     4741 tctgatgatg tggcgtaaaa aataaaaaca tgcagtcagc caccacacca taaattttag
     4801 gtgagtcaaa gctacgacac aaaaaaacac gcctggcgcg tgttgtgtcg ccgttagatt
     4861 acacgggttc ctacgcccgg ctgccgattt tgcggcaacc tttaaactat atcccgcgca
     4921 ttcctggtga ggcaagaaat ttataaccga aaactgaaca ttgcctggat taattaacgc
     4981 tggcatgctt aagcctctgc ttaaaaatag ttcttcattt ttcctgatgc tgtaaacgag
     5041 acccgcttct gagcgggtct ttctgtcgcg aagtcaatca gcaaacgtca gggcgctctc
     5101 atcaacagcc gcgtacagtt caggttcacc gagtgcatag ccttcattgc gcaggtagga
     5161 atgtacgtat tcactgtttt catcatcaaa ctcagcacga aaccaccgtg ttatcgcctg
     5221 agtgtaaata tgcagattat ccagcgtcgt tactgccaga cgcttacccg gcataaacgg
     5281 gggaataaac gcaaagcggc cagcaacaga acttgtagcc atctgtgcag cattcacatc
     5341 agccgggcgg tctgcggcat taaataactt caggcgttga tgtgctgcca gctctgcccc
     5401 aaccagtaca accagacggg gatcttcacg aaactgctca gcaataagct cagtaatcag
     5461 atggttggcc agagaatcaa tatttttcca ggtgccagtc tctcccagag tgacaggctc
     5521 agagatgatt tgctttccgt tcccgtaggc tttcgccagt gcatgccagc caatattgac
     5581 atcctcgcct tttttattga tttccgggtt tgtagtattt gcaatcgaca caccattgaa
     5641 accgacccgc agcatgtcca gcgcatatgc ctgagcaaaa aagctctcaa ccgttttctc
     5701 aaactcatcc ggcacaccgg tatggtaaat atgagacata tcggaatagc tgatccgggc
     5761 gcaggtatcc gtctctgtca gaaagaactc cgtaccattg attgccattt ttttgtggaa
     5821 acggccacca tccacacgtc cggtatgcag ttcactggca ccaatattga cggcattacc
     5881 tgacacggcg ttaacatcac gtagggtgat atttttaata agccacccgg attccagaat
     5941 tgaggtacgc agtatatttt cagtgggttc gcttaattca aagcgtctgt ttgattcttc
     6001 ctgatagtta gtgccagtga cgaatttatt atgataacga cgtgctgcgt ccggcctttc
     6061 agttttgaag gcatacatag tttttcacct ttttaagtat ttaagaaaag attttatttt
     6121 tgtactcagt aacccgattg tatttcatta catgctcaac ataatcccgg tcacttacga
     6181 ctgaacgtaa tacataatta tctgctcgat agtactccct gtaagccata gctcttgcca
     6241 ttccaccgtt atttaataca taacgatcag ttggaacgct acaacggcta ataatttcac
     6301 tcacagaatc agacaaagat ttgagtgcat ctgtaataaa tttaatatca cggacaacgt
     6361 caccgtcatt aaaaacagaa ataaaatcag cagggatctg gccggattga ggaaatatgt
     6421 tcagtaaacg cttctcaact cccttaccaa tgtagttaac ttcatcggcc agtacgcccg
     6481 gtaaaagctt gattgtgctc agtcggctat gttgctcatt cagccgggaa acagccagat
     6541 gataacaccg ttcagcatcc gctcgttcag catgccccgt tttattcgtt ttggcttcag
     6601 caatgctact gacgccgttt tttgaggatg tgcttttcca tgcaacttcc gcatttgtca
     6661 cttcacgctg ccgaaaagat aactcttcct cttcctgacg acacaactgg gcataacggg
     6721 tcaggagcag gttgatctca ttcgccacag cttccgtatc aggtactgtt aaccccgtaa
     6781 gtgaatacag cttttcacct atagcacgca gcttattcag tgaatagatt tcgataatac
     6841 gcacaataca ggctatgtcg ctatgagttt ctttaaatgg gcttactttg taactatcta
     6901 acaccgacac atgttcgttc ttaattgatg ccagtttttt cttaaaaaca ttgtcaatca
     6961 tataattgcc acctcaattt aggagtttaa ttttctattg agaatatttt tctctatcca
     7021 ttcattaact tcatgctcca cccatgcaac agaacgagaa ccgatagaaa cttggttcgg
     7081 aaattcatca ttctttatct ttagataaat tgttgacttg ggtaagccag tcaaattaat
     7141 cacttcagat attctcagaa gtctatagtt tttttggggt gcgttttccc gaggcaataa
     7201 gc
//
LOCUS       DAMZII010000195         7180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZII010000195 DAMZII010000000
VERSION     DAMZII010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.195"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            117..1451
                     /gene="cadB"
                     /locus_tag="POA77_004444"
     CDS             117..1451
                     /gene="cadB"
                     /locus_tag="POA77_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDI6367776.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1531..3678
                     /gene="cadA"
                     /locus_tag="POA77_004445"
     CDS             1531..3678
                     /gene="cadA"
                     /locus_tag="POA77_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDI6367777.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3737..5194
                     /gene="dtpC"
                     /locus_tag="POA77_004446"
     CDS             3737..5194
                     /gene="dtpC"
                     /locus_tag="POA77_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDI6367778.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5431..6948
                     /gene="lysS"
                     /locus_tag="POA77_004447"
     CDS             5431..6948
                     /gene="lysS"
                     /locus_tag="POA77_004447"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6367779.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7067..>7180)
                     /gene="ghoT"
                     /locus_tag="POA77_004448"
     CDS             complement(7067..>7180)
                     /gene="ghoT"
                     /locus_tag="POA77_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDI6367780.1"
                     /translation="IIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1768 a         1680 c         1758 g         1974 t
ORIGIN      
        1 tctttaggat taatcctttt ttcgtgagta ttcttagcgc cagtttggtc tggtcaggaa
       61 atagttatac atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga
      121 gttctgccaa gaagatcggg ctatttgcct gtaccggtgt tgttgccggt aatatgatgg
      181 ggagcggtat tgcattatta cctgcgaacc tagcaagtat cggtggtatt gctatctggg
      241 gttggattat ctctattatt ggtgcaatgt cgctggcgta tgtatatgcc cgactggcaa
      301 caaaaaaccc gcaacaaggt ggcccaattg cttatgccgg agaaatttcc cctgcatttg
      361 gttttcagac aggtgttctt tattaccatg ctaactggat tggtaacctg gcgattggta
      421 ttaccgcggt atcttatctt tccaccttct tcccagtatt aaatgatcct gttccggcgg
      481 gtatcgcctg tattgctatc gtctgggtat ttacctttgt aaatatgctc ggcggtactt
      541 gggtaagccg tttaaccact attggtctgg tgctggttct tattcctgtg gtgatgactg
      601 ctattgttgg ctggcattgg tttgatgcgg caacttatgc agctaactgg aatactgcgg
      661 ataccactga tggtcatgcg atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg
      721 gtgttgaatc cgcagcagta agtactggta tggttaaaaa cccgaaacgt accgttccgc
      781 tggcaaccat gctgggtact ggtttagcag gtattgttta catcgctgcg actcaggtgc
      841 tttccggtat gtatccatct tctgtaatgg cagcttccgg tgctccgttt gcaatcagtg
      901 cttcaaccat cctcggtaac tgggctgcgc cgctggtttc tgcattcacc gcctttgcgt
      961 gcctgacttc tctgggctcc tggatgatgt tggtaggcca ggcaggtgta cgtgccgcta
     1021 acgacggtaa cttcccgaaa gtttatggtg aagtcgacag caacggtatt ccgaaaaaag
     1081 gtctgctgct ggctgcagtg aaaatgactg ccctgatgat cctcatcact ctgatgaact
     1141 ctgccggtgg taaagcatct gacctgttcg gtgaactgac cggtatcgca gtactgctga
     1201 ctatgctgcc gtacttctac tcttgcgttg acctgattcg ttttgaaggc gttaacatcc
     1261 gcaactttgt cagcctgatc tgctctgtac tgggttgcgt gttctgcttc atcgcgctga
     1321 tgggcgcaag ctccttcgag ctggcaggta ccttcatcgt cagcctgatt atcctgatgt
     1381 tctacgctcg caaaatgcac gagcgccaga gccactcaat ggataaccac accgcgtcta
     1441 acgcacatta attaaaagta ttttccgagg ctcctccttt cattttgtcc catgtgttgg
     1501 gaggggcctt ttttacctgg agatatgact atgaacgtta ttgcaatatt gaatcacatg
     1561 ggggtttatt ttaaagaaga acccatccgt gaacttcatc gcgcgcttga acgtctgaac
     1621 ttccagattg tttacccgaa cgaccgtgac gacttattaa aactgatcga aaacaatgcg
     1681 cgtctgtgcg gcgttatttt tgactgggat aaatataatc tcgagctgtg cgaagaaatt
     1741 agcaaaatga acgagaacct gccgttgtac gcgttcgcta atacgtattc cactctcgat
     1801 gtaagcctga atgacctgcg tttacagatt agcttctttg aatatgcgct gggtgctgct
     1861 gaagatattg ctaataagat caagcagacc actgacgaat atatcaacac tattctgcct
     1921 ccgctgacta aagcactgtt taaatatgtt cgtgaaggta aatatacttt ctgtactcct
     1981 ggtcacatgg gcggtactgc attccagaaa agcccggtag gtagcctgtt ctatgatttc
     2041 tttggtccga ataccatgaa atctgatatt tccatttcag tatctgaact gggttctctg
     2101 ctggatcaca gtggtccaca caaagaagca gaacagtata tcgctcgcgt ctttaacgca
     2161 gaccgcagct acatggtgac caacggtact tccactgcga acaaaattgt tggcatgtac
     2221 tctgctccgg caggcagcac cattctgatt gaccgtaact gccacaaatc gctgacccac
     2281 ctgatgatga tgagcgatgt tacgccaatc tatttccgcc cgacccgtaa cgcttacggt
     2341 attcttggtg gtatcccaca gagtgaattc cagcacgcta ccattgctaa gcgcgtgaaa
     2401 gaaacaccaa acgcaacctg gccggtacat gctgtaatta ccaactctac ctatgatggt
     2461 ctgctgtaca acaccgactt catcaagaaa acactggatg tgaaatccat ccactttgac
     2521 tccgcgtggg tgccttacac caacttctca ccgatttacg aaggtaaatg cggtatgagc
     2581 ggtggccgtg tagaagggaa agtgatttac gaaacccagt ccactcacaa actgctggcg
     2641 gcgttctctc aggcttccat gatccacgtt aaaggtgacg taaacgaaga aacctttaac
     2701 gaagcctaca tgatgcacac caccacttct ccgcactacg gtatcgtggc gtccactgaa
     2761 accgctgcgg cgatgatgaa aggcaatgca ggtaaacgtc tgatcaacgg ttccattgaa
     2821 cgtgcgatca aattccgtaa agagatcaaa cgtctgagaa cggaatctga tggctggttc
     2881 tttgatgtat ggcagccgga tcatatcgat acgactgaat gctggccgct gcgttctgac
     2941 agcacctggc acggcttcaa aaacatcgat aacgagcaca tgtatcttga cccgatcaaa
     3001 gtcaccctgc tgactccggg gatggaaaaa gacggcacca tgagcgactt tggtattccg
     3061 gccagcatcg tggcgaaata cctcgacgaa catggcatcg ttgttgagaa aaccggtccg
     3121 tataacctgc tgttcctgtt cagcatcggt atcgataaga ccaaagcact gagcctgctg
     3181 cgtgctctga ctgacttcaa acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg
     3241 ccgtctctgt atcgtgaaga tcctgaattc tatgaaaaca tgcgtattca ggaactggct
     3301 cagaatatcc acaaactgat tgttcaccac aatctgccgg atctgatgta tcgcgcattt
     3361 gaagtgctgc cgacgatggt aatgactccg tatgctgcgt tccagaaaga gctgcacggt
     3421 atgaccgaag aagtttacct cgacgaaatg gtaggtcgta ttaacgccaa tatgatcctt
     3481 ccatatccgc cgggagttcc tctggtaatg ccgggtgaaa tgatcaccga agaaagccgt
     3541 ccggttctgg agttcctgca gatgctgtgt gaaatcggcg ctcactatcc gggctttgaa
     3601 accgatattc acggtgcata ccgtcaggct gatggccgct ataccgttaa ggtattgaaa
     3661 gaagaaagca aaaaataatt agctcgtaca agggaagtgg cttgccactt cccttttttg
     3721 ctcataagga gaacacatga aaacaccctc acagccgcgc gcgatatact atatcgtggc
     3781 gatccaaatc tgggagtact tcagttttta cggcatgcgt gccttactca ttctctatct
     3841 cacccatcag cttggttttg atgataacca tgccatcagc ctgttcagcg catatgcttc
     3901 tctggtttac gttaccccta ttctcggcgg ctggcttgcc gaccgcctgc tcggcaaccg
     3961 cactgcagtg attgccggcg cgctgttaat gacccttggc catgtggtgc tgggcattga
     4021 tacaaattca acctttagcc tgtatctggc gctggcaatc attatttgtg gctacggttt
     4081 attcaaatca aacatcagct gtttgcttgg cgagctctac gacgagaacg atcatcgacg
     4141 tgatggcggt ttttcgctgc tgtatgctgc gggcaatatc ggttctatcg ccgccccaat
     4201 cgcctgcggc ctggctgctc agtggtatgg atggcatgtt ggctttgccc ttgcgggtgg
     4261 cggcatgttt atcggtttgt tgattttctt aagcgggcat cgtcatttcc agtccacgcg
     4321 tagtatggat aaaaaagcgc tcactagcgt caaatttgcc ttaccagtat ggagctggtt
     4381 agtggtgatg ctctgtttag ccccagtatt ttttaccctg ctgctggaga acgactggtc
     4441 cggatatttg ctggcgatcg tttgcctcat tgccgcacaa atcattgccc gcatgatgat
     4501 caaattcccc gaacaccgcc gtgctctttg gcaaattgta ttgttgatgt ttgtcgggac
     4561 gttgttctgg gtactggcac aacagggcgg cagtaccatc agcttgttta tcgatcgctt
     4621 tgtgaatcgt caggcattca atattgaagt acctacagca ctattccagt cggtgaatgc
     4681 aattgcagtg atgctcgctg gggttgtgct ggcctggctg gcatcgccag aaagccgcgg
     4741 caactcaaca ttgcgcgtct ggctgaagtt tgcctttggc ttactgctga tggcttgtgg
     4801 ctttatgttg ttggcatttg atgcccgaca tgcagcggct gacggtcaag cgtcaatggg
     4861 cgtgatgata tccgggttgg cgctaatggg ctttgccgaa ctctttattg acccggtggc
     4921 gattgcgcaa atcacgcgtc tgaaaatgtc tggcgtatta accggtattt atatgctggc
     4981 aacaggcgcg gtcgccaact ggctggcagg cgtcgtggca cagcagacga cagagtcgca
     5041 aattagcggt atggcaattg cagcttacca gcgattcttt tctcagatgg gagagtggac
     5101 gttggcttgt gtcgcgatca tcgtcgtatt ggcctttgct acccgttttc tgtttagcac
     5161 gccgacgaat atgatacagg agagcaacga ttaactgtcg taaaaacaat tggttatgaa
     5221 taaattaact ttcagttaat cacactagta aatcgagatt aataagtcat ttaaaccatt
     5281 ttgatggtta tttattagtg atatcaactt gaggtaagcg ttagtttcga taagataaac
     5341 tgagttatta atagtcgagg cagataatac agtgtaccga tctgtctctt tatctacgct
     5401 aaattgaaag ctggatttag aggaaccaaa atgtctgaac aagaaacacg gggagccaat
     5461 gaggctattg attttaacga tgaactgaga aatcgccgcg aaaaactggc agcactacgt
     5521 cagcaaggtg tggcgtttcc caatgatttt cgccgcgacc atacctctga ccagttgcac
     5581 gaagagtttg atgcgaagaa taaccaggaa ctggaatcct taaacattga agtctcggtt
     5641 gctggccgaa tgatgacccg tcgtatcatg gggaaagcct cctttgtaac gttgcaggat
     5701 gtcggtggcc gtattcaact gtacgttgca agagatagcc tgccagaagg tgtttataac
     5761 gatcagttta aaaaatggga tctgggtgac attatcggtg cccgcggtac gctgtttaag
     5821 acgcaaaccg gtgagctttc cattcactgt actgagttgc gcctgctgac aaaagcacta
     5881 cgtcctttac cagataaatt ccatggtctg caggatcagg aagtccgtta tcgtcaacgt
     5941 tatctggacc tcatcgctaa cgataaatcc cgtcaaacgt tcgttgtccg ttcaaaaatt
     6001 ctggccgcta tccgtcagtt catggtcgcg cgcggcttta tggaagtaga aaccccgatg
     6061 atgcaggtaa ttccaggtgg ggcatctgct cgcccgttta ttacccatca taatgctctg
     6121 gatttagata tgtatctgcg tatcgcgccg gagctgtatc tgaaacgtct ggttgtaggc
     6181 ggttttgaac gggtattcga aatcaaccgt aacttccgta atgaaggtat ttctgttcgc
     6241 cataatcctg agttcacaat gatggaactc tacatggcgt atgcggatta ccacgatttg
     6301 attgaactga cagagtccct gttccgcacc ctggcacaag aggttctggg taccactaaa
     6361 gtcacttatg gcgagcatgt gtttgatttc ggcaaaccgt ttgaaaaact caccatgcgc
     6421 gaagcaatca aaaaatatcg tccagaaacc gatatggccg acctggataa ttttgatgct
     6481 gctaaagcat tagctgaatc tatcggaatt acggtagaga aaagctgggg gctgggacgt
     6541 attgtcacgg agatctttga tgaagtggca gaagcacatc tgattcagcc aacctttatt
     6601 acggaatatc cggcagaagt gtcaccgctg gcgcgccgta atgatgttaa cccggaaatc
     6661 accgaccgtt ttgaattctt catcggtggt cgtgaaatcg gtaatggttt tagcgaatta
     6721 aacgacgcag aagatcaggc tgaacgtttc caggaacagg ttaacgctaa agctgcaggt
     6781 gacgacgaag ccatgttcta tgacgaagat tacgtgactg cgctggaata tggtctgccg
     6841 ccaaccgctg gtctgggtat tggtatcgac cgaatgatta tgctgtttac aaacagtcat
     6901 actattcgcg acgttattct cttcccggcg atgcgcccac agaaataaat ttcactttaa
     6961 tgaacgaagc agtcaggcga ctgcttcgtt cgtcgttccg caaatccagt tttgaggaga
     7021 gacgcttcgt ttcacgataa ccagcgggtc cactcaacta cagctcctaa aagagagaaa
     7081 aaagtaatgc cacaggcaga ctcattggcc aggttattcc gaccaggaat gcactaagta
     7141 aacgaatatg tgttttctca tgtgagataa accagataat
//
LOCUS       DAMZII010000196         7145 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZII010000196 DAMZII010000000
VERSION     DAMZII010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.196"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(186..2969)
                     /locus_tag="POA77_004449"
     CDS             complement(186..2969)
                     /locus_tag="POA77_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6367781.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3026..5397)
                     /locus_tag="POA77_004450"
                     /pseudo
     CDS             complement(3026..5397)
                     /locus_tag="POA77_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(5435..7120)
                     /locus_tag="POA77_004451"
     CDS             complement(5435..7120)
                     /locus_tag="POA77_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6367782.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1854 a         1622 c         1599 g         2070 t
ORIGIN      
        1 gtaaggtgtt ttatgctgtt atttttatgc gcattctgtg tctcctgaat tatcacgtaa
       61 aaatcagacc ttaaaatatc actattagta cttgattatt attttgaacg catttataaa
      121 attattacat aaaaatagcg aatattgcta aaatccccgc caacgatgtg ttgacggggc
      181 tgttattatt ttggcaataa tactccggta taagtattta ccggatgaga aagatattgt
      241 ttaacggcag tgttaacatt ctctaccgtc atttgtttca acaattgctc ctgctcagtc
      301 catgctgcag gatcgtcata ttgaataaga ctatttacaa tagtgttcgc taattgttga
      361 acgctacgct gttggatatc gaggctgcgc tgaacgtttt gctggtattc attcagttct
      421 tgctcactga tccctttagc cagacgctta accatcactt cattcgctaa cgttaacagt
      481 tcatcatgtc gttctggttg acaagtaaaa gccagcaaat gactgatatc tttggcctga
      541 ggatcaaccg agaggcgaga agaaacgctg tatgctccag atgcctgttc acgaatatta
      601 acacgtagat cttttgccag tgcgacgtta aaagcatcga gcgccatacg cgtcgccaga
      661 ttaacaggtg tccgggaatc ataacgcttc cactgtgaaa actgtgccac aggttcattt
      721 tgttctttta cagtaaccga tgcgttgtcc gtcgcgcgag ttaatggttt acctgcggct
      781 aatggcgaat cagagtgttt gattgatcct aagtaacgcg taattaacgc cacgagtttg
      841 tcttctgaga cattaccgac aatgacaaac gtgatatccg ctggagatga aaacaactgg
      901 cgatcggcag ccagcgcatc tgcggcagta aactgtacaa tctgattttc ttgcagtaat
      961 ttcgtgcggt catcagcata gcgcgtctca tacatctgct gggcgaattt ctccgctgga
     1021 cgctggtcga gcgttttcaa cgcctgaatt tgagcatttt gtagcgatgc ccaaatattg
     1081 tcgttaatcg tgctgtgggt gattcgctgg ttaatcaact gaaaaccagg ttcagggtta
     1141 ttagtccgcg cgctaacgga gagcaacgta ttcatgccac tgactttact gctcatggtt
     1201 accgaatttt ccgcactcca gcgtttcagg ctggaagagg agagctcacc aacgccgctt
     1261 ccgcttactg ctttatttgc cagcgcgata agtgattttt gctgcgcagg gaaacttaaa
     1321 tcgcctttat tcgatacggc agtaatttgt agcttttgct cttcacccgc ggattttgcc
     1381 agaataaccc tggcaccatt ggaaagtgtt aatgatgtca gattctcagc cagcgtttct
     1441 ttgctgctaa tttccgcctg tgggtcagcg tctactgtta acgataaatt tctgcctggg
     1501 aagatgtaag ccgccagctt tttgttggcg tactcttttt ccagcgccag gatagccgca
     1561 ggagacaatg cttttttggc ggcaagctca ttgtttacca tttgctccca aaatgcgtcc
     1621 tggttctttc ttaactgctg ccatttttcc gccagacttt gcacggtaat ttgctgccac
     1681 agacgtttcg aaagttgata tgtctcttcc ggcgacaaga acggcgtatt atttaatgag
     1741 ctggatgcca ggcgactggt cagcatacgt aaatcacgtt cagcttgctg atcaaccgca
     1801 tttttcagcc aggtgaggcg ggtagatttg acatcatcga gttcttcagc agaaaaacca
     1861 tgctgatcaa tggttgccaa ctctgccatt aatgcattcg cagcatcctg catattatcg
     1921 tctcgtgcat ttacacggaa aaacagcgac tgataatcgg gtgcaatttt aacgctgcgc
     1981 gcagtgccgc cagaaatagt cttcaactcg cccgactgta tgcgttcctg cagacgttga
     2041 ttgaacagct gaactaacat gctccattca gcttgttcga taaagctttg ctcatcgttc
     2101 acttgtacca ttggcaggcg ataatagagt gcgatgccgt tcacccggtt ttctttatca
     2161 ttaatgatat taaagcgcag gtggttttcg gcttttgtcg gccagacgcg attttcagct
     2221 gctttgttag ccggaagctt acttaaatta tcctttatca gcgccagcgc ttctttactg
     2281 tcgatatcgc cgaccacgat aaaggtcata ttatttggtt gataccagcg ttgataaaat
     2341 tggcgcaatt gtgccggtgt gaccgtggcg acagtatcca tcaggccgat aggttcacgg
     2401 tctaaattac gggtattagc cagcaggaaa gggcggcgcg cctgagaggt gcgccatttc
     2461 gcatcctgat gggcacgcca ttcctcagta attacgccac gttcagcgtc tacttcgagt
     2521 ttttcaaagg ttgcggcatt actccattca ctgaagattg ccatcacttg ttgcagattt
     2581 tgtttctgcg tagtcggcaa actcacctga tacaccgttt cgtcatagct ggtataggca
     2641 ttaacatcgc gaccaaaacg caggcccatt gactcaaatg tttcgatgac tttattaccc
     2701 ggccatgttt ttgtgccgtt aaacatcata tgttctacaa aatgagccac gccgcgctca
     2761 ttgtcttctt cctgcaatga accggtatga atttgcagcc ataaatttac ctgatccttt
     2821 ggatgagcat gcggataaat catatatcgc aagccattgc ccagttgccc ggtaattaac
     2881 ttttcatcct gcggtaaggc ggcggcaatc agccgcccgg ggagcaacag agttgccact
     2941 aacgtcagta agaaacagag gtttctcata attatctcca tgcgaaaacc gggcgaattt
     3001 acccggttaa gtaaaatccg aactattaaa atttcatgct gacatcaagc cagaaagtac
     3061 ggccactggc gtaggtcgcc acaccggtgt tcgttgtatc aacagcggtt ttgctatcca
     3121 gtacattgag aatatcggca ctgatcgtca ggttttgttg tttcagaaat tgtggcgtcc
     3181 aggacaagcg ggtgtcccag gtcaggctgc tatccagttt ttcgtcaaca tactgcttgt
     3241 aatctgaata ttcgctgatg tattgcgcat ttgtcttacc aaggataatg cgagctttac
     3301 gcgcttcttg ccaggccagc gtattcgccc acactaaccc gctcggttga tgcgtgaaat
     3361 ccatgtttaa ggagatcttt aatgggttat taaaatctgc cactggaacg ctatcgtaag
     3421 agaccagatt accgttataa accacctggt tatcaccggt attgctctcc tcataaccat
     3481 tgtttaacga caagttgccc ttgctcttgt tatagctaaa gacaatttgt gggttaatat
     3541 ctacctggcg gatatgcagg ggttcggcca gttcaaaact gaggctgaac gaatgcgttt
     3601 tggttttgcc atcgttgtta tattcagtaa tggtggtttt agtcgcgctg tcggtacgac
     3661 tgcttttgct gatttgatca tgcgcttcac ggtaaacata gtttgcgcgt gcaataacgt
     3721 tcttaccgat tttttgctgc aatcccattg ccagttcatc gttataaggc gttttcaaat
     3781 tctgataacg cgtcagggtt ttattacctg ataccgattc cgtccagcta ttgcggatat
     3841 cacgtaatcc catatcaaga atattcccgc catagtaacg gttataaccg gcggtaatca
     3901 ttgaggtttg attagcaaaa atatcccatt ccgtcataaa gcgcggggag atattgtggt
     3961 ttgacagata gttgtcatag tcgtaacgca caccgggcat taacgacaca ttccgccagc
     4021 taatgtgatc cgccatatac agcgtgtagt tgtcaattcc caggctgcct ttacctttat
     4081 ggtaaatggt atggttagtt ttctttcctg cagcattaat cacataggat tcagactggt
     4141 tatggcgttc agtccacgca tcggaataga tgtattccgc gccgaagtag ggttgatgcg
     4201 aaacattacc cacggcgaat ttttgccagt ccaggcgtgt tttgaaggtg taattatcta
     4261 cagcctggga aatgtgtcct aatccgccac gggtgcaacg ccctgtaata tcaccatatg
     4321 tacatgaaag ttcggtgtac cagatgtcat gatcgtgacg ggtataatca ctaatatgat
     4381 cccaaccaac ggtggtacgc agtttggccc atgcgagctg cgtatccata tcccatgcca
     4441 gaccgtagga tttattacca tttcgcgatc agactgcggg aaggtgctgg tattataatc
     4501 acggctggag ccggtatatt ttaaggttaa atcgtgggta aagcggtcgc tggcaaacca
     4561 ggtaaatttg ctcaatgcag tatcgataac gtttttatac tgtgcccgac cggcgacaat
     4621 gccgttattc gaaacataat ccgcgcgggt gatatcagac tggcggcgcg ataaaccggc
     4681 ggtaacgccg aagttatcag cgagttcctg attaaacgac aaggtataaa agttcttttt
     4741 aaaatctggg gagtaataag tacttcctga agagccttga ttaaatgcgc tcttgttatt
     4801 ctcatcgata tgcgatgtta accagtccga acgcgtagtg cgataaccca atttaacctt
     4861 gctatcatca gcgttgaagc gtttgatctt tgcatcaatt accccgccat tgaagcgacc
     4921 aaattcaacc ggcacaaaac tgtcataaag cgtcacattg tccagtaagc tgacatcaag
     4981 ataatacccc tgtgacatcc cgctaatatt ggttgcacta ctggcatcgg actcattcgc
     5041 tgggttcagg ttattagttg cactaatacc gtcaatcaaa taggcattct ggtagggcga
     5101 cgcaccgtga atagagattt tctcagggcg aatatctccc tggttcaacg aggtactttg
     5161 cgttgaatcc atgcgtaccg cagggttggt tctcagcaga tcgctgatat tgccattccc
     5221 ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg ttgccgttta cgggtacagg
     5281 gacaggagca tagaccgtca tctcttcaaa aaaatgcatg tacatgtttt tgtcatcgac
     5341 ggcctgcgcc acatcagcgc cacataaaat gacgccagga ataagaactc gcttcattta
     5401 tttatatctc cgtacagcag ggcttttatt gttgttataa aaccgtgcta atgtcacaaa
     5461 tatcatcagc caggttccag acaccgggtt gatgtgtaac cataatgacg ccgcttgtgg
     5521 gtagtttttc acgcactaaa cgcagtaagc gtatagcctc ttgttcctca agatgagagg
     5581 tagtttcgtc aagaaatatc catttcggac gtcgtaaaat taatcgtgcc agggcgatac
     5641 gttgtttttc gccgctggaa agaatatctc cccagcgatc gtgctcatga atacgcgcag
     5701 ccaatttccc cagaccaacc tgatgcagta cttcgctcaa cgatttatcg tctacgggca
     5761 ggggaagtgc tttacaaata atctctttca gtaagccgct tttgattaac ggtgtttgtg
     5821 acacatacca actgtcagca ggagaagaaa tatcaccttt aaaccacggc cagcagtggg
     5881 ataatgtttt aagcagtgtg gtttttcccg caccagagta gcctttcagt aatagccatt
     5941 tgcctggcga aacatgaaag ttcaagttct ctaatatgat cttattatca ggcgtacgaa
     6001 tactcgcatt agccacttgt accgcatgtt ggcaattttt aggcttattc gtagggcgct
     6061 gttcagtgag ttgatggaac tcatacaagc gatcgataac cgcagccagt tcagcaagtt
     6121 cgtcatattt ataaataaac cagcttaaat tgttcgatac tagcataaat gcctggcgcg
     6181 atttcatcag tccgcccaga ttgatctgcc cgctaataaa ctgcggcaat aacagaaagt
     6241 agggaagaac gctaagcgag cgcgaataaa tattttgcca gtaatcaagc caccgctgac
     6301 gattcattaa acgatgccag ttctctttaa tggtatgaaa attgtcgcgc aactcctggc
     6361 gttgtagaga ctcagcattg cttagcgcaa tcagttctgc ctgcttatta tgctgcacaa
     6421 gattagttcg aaatgtcgct tcgctacgtt gtttttccac attaagcgga cgaatacgtt
     6481 tacccacctt atgggtaaat aaagttccac cgatcacaat gagcacgacg gtatagacca
     6541 tatacccctg gatattccat tctgttccac caacagtgaa tgagagcgta cccgcgcttt
     6601 gccaaagaat aacggtaaag gtgatcagca tgctaagtga ctggatgaag ccaaatgaaa
     6661 gactgagcgt tttgctgatc agtaagagaa tgtcttcagc gatacgttgg tcagggttat
     6721 ctgtattttt atgctcgccg tagatctgcg tgaagtagta gtttttatct gcaaaccagc
     6781 gattaaggta gtaatctgtt aaccattcgc gccagcgaat agtcagtagt ttaattaacc
     6841 atgttttgtt tacggatatt aatacaaaga tccccagcag ggcagggaac caaaggacaa
     6901 gctgccaaag cttatcggtt tctttctggc ttagcgcatt gaaaaaatca ttattccagt
     6961 cgtttaacca gacctgaatt ttaaccacgc caaggatcat tgcgaggata ataatgatta
     7021 acaacactga agttttatta ttcttacgca gccaaaaggg cttcagcaga cagagatact
     7081 ttgctatcaa catacaaaac gtaatgggaa tggttatcat tagcgaaaat tgattgtgcc
     7141 atcca
//
LOCUS       DAMZII010000197         7123 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZII010000197 DAMZII010000000
VERSION     DAMZII010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.197"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            380..1417
                     /gene="dusA"
                     /locus_tag="POA77_004452"
     CDS             380..1417
                     /gene="dusA"
                     /locus_tag="POA77_004452"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDI6367783.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1551..1793
                     /gene="pspG"
                     /locus_tag="POA77_004453"
     CDS             1551..1793
                     /gene="pspG"
                     /locus_tag="POA77_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDI6367784.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(1959..2942)
                     /locus_tag="POA77_004454"
     CDS             complement(1959..2942)
                     /locus_tag="POA77_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDI6367785.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3025..4440
                     /gene="dnaB"
                     /locus_tag="POA77_004455"
     CDS             3025..4440
                     /gene="dnaB"
                     /locus_tag="POA77_004455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6367786.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4493..5572
                     /gene="alr"
                     /locus_tag="POA77_004456"
     CDS             4493..5572
                     /gene="alr"
                     /locus_tag="POA77_004456"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6367787.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5825..7018
                     /gene="tyrB"
                     /locus_tag="POA77_004457"
     CDS             5825..7018
                     /gene="tyrB"
                     /locus_tag="POA77_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDI6367788.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1675 a         1762 c         1896 g         1790 t
ORIGIN      
        1 taaatcggcg aaataatatg tgaagttctt cgatggtatg gaaggcatta cataaaagaa
       61 cctaatactt attgggttct tttttcttct atcagtgcca ttagcaggaa gagatatcac
      121 cggagtttaa tgtgtgattt tttatttatc gtcgaacctg gattgtttat cattgtcctt
      181 aacaaggcta acggctaata agattatttc catcacttcg tgagagcttc atgccttgat
      241 ttgatctcaa ttttcttttg caatgagaca ggcgcttcct gttgttatgg tatagtaccc
      301 cgctattgag cctcctgaac agtgatgctg aataacataa cccaatgata tatcgataaa
      361 ataatctcta catttgaaaa tgcacggtaa ttctgaaatg caaaaaatca accaaaccag
      421 cgcaatgcct gaaaaaactg acgttcactg gagtggtcgg tttagcgttg caccaatgct
      481 cgactggacg gacagacatt gccgctattt cttgcgtctg ctttcccgca atacgttgct
      541 gtataccgaa atggtgacca caggggcgat tattcacggt aaaggtgatt acctggcgta
      601 cagtgaagaa gaacatccgg tagcgttgca actcggcggt agcgatccgg cggcgctggc
      661 acagtgtgcg aagctggcag aagcgcgtgg atatgatgag atcaacctga atgtcggctg
      721 cccgtctgac cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta atgcgcagct
      781 ggttgccgac tgtgtgaaag cgatgcgcga tgtggtgtcg attccagtga cggtgaaaac
      841 gcgtattggc atcgatgacc aggacagcta tgaatttctc tgcgatttca tcaacactgt
      901 ttccggcaaa ggcgagtgtg agatgtttat catccacgca cgtaaagcct ggctttcggg
      961 gttaagcccg aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg tgtatcaact
     1021 gaagcgtgac tttccgcatc tgacaatgtc gattaacggt ggtatcaagt cgctggaaga
     1081 ggccaaagca cacctgcaac atatggatgg cgtgatggtc gggcgcgagg cgtatcagaa
     1141 tccgggtatt ctggcggcgg tagaccggga gatctttggt tcctcggata ccgatgccga
     1201 tccggtggcg gtagtgcgcg ccatgtatcc gtacattgag cgtgaactca gccaggggac
     1261 gtatctcggc catattaccc ggcatatgct gggtttgttc cagggtattc ctggcgcgcg
     1321 gcagtggcgg cgttatttaa gtgaaaatgc ccataaagcg ggtgctgaca ttaacgtgct
     1381 ggaacacgcg ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa agtagtcaaa
     1441 ttcaccacgc cctgcgcacc gtcgcggggc gttttgctgt taaatcaata gattattttt
     1501 ggcatgattc ttgtaatgcc agcaagagat ttcatatttg ggagagcatc atgctggaac
     1561 tactttttgt gattggcttt tttgtcatgc tgatggtcac cggcgtttcg ttgctgggca
     1621 ttatcgccgc gctggttgtg gcgacggcca ttatgttcct cggcggtatg ctggcattga
     1681 tgattaagtt gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt attaaggcga
     1741 ttaaagcacc aaaaatgccg aaatatcagc gttatgaccg ctggcgttac taagggattg
     1801 tgcggatgat cacaacctaa ggttttatcc ttagaacaaa ataggaattg ataatcaaat
     1861 ctgtcactat tgcgcctcta acagattcat cgtgctgtac cctacataca gccgaactat
     1921 aaaaagaaag ggcttcccag gtggaagccc tatttctttt atggaatcag caggctggaa
     1981 ccttgcgttg cccggctttc cagaatttca tgcgcacgct gcgcatcctt cagcggatat
     2041 ttctgctgct cggcgacatc gaccttaatc acaccgctgg caatcaaaga gaacagttca
     2101 ttactggcct cggttaattc ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc
     2161 acatacaacg agcctttctg attgagaatg cctaagttca caccggtaac cgcacctgat
     2221 gagttgccaa aactcaccat taagccgcgg cgttgcaggc aatccagcga ccgttcccag
     2281 gtgtctctgc ccacggaatc gtacaccacg cgcactttct taccgccggt gatctctttt
     2341 aaccgctcga ccagattctc ttcacgatag ttaataacct gccacgcgcc cgcttttagc
     2401 gcgctctgcg ctttttgcgc ggttcctacg gtgccgataa gtttcgcgcc cagggctttt
     2461 gcccactggc aggcaatcaa gccaacgccg ccagctgctg cgtggaacag gaactgctca
     2521 tcgggtttaa tttcataggt tttgcgcagc agataataaa ccgttaagcc tttcaggaag
     2581 gatgccgcag cttgctcaaa agaaattgcc gcaggcagaa tcgccgcttt atccgcaata
     2641 atgttatgca cagagctgta agcgcctaac gccgactgcg catagactac acgatcgcct
     2701 gccttaatat gctttacacc actgccgact ttactcacga tgcctgctgc ttcggtgcct
     2761 aatccgctgg gtagcgatgg cggcgggtaa aggccgctgc ggatatatgt gtcgataaaa
     2821 ttgatgccga tggctttatt ttcgacctgg atttcattct ctgccggatc ggcaggagtg
     2881 aactctacgg cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc
     2941 atgtgtcctc cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt cgacccattc
     3001 tttatctcgg taactccatt cactatggca ggaaataaac ccttcaacaa acagcaggct
     3061 gaaccccgcg aacgcgatcc acaagttgcc gggctgaaag tgcctccgca ctcgatcgaa
     3121 gcggagcagt cggtgttggg cggtttaatg ctagataacg aacgctggga tgatgtagcc
     3181 gagcgtgtgg tagcagacga tttttacacc cgcccacacc gccatatctt tactgaaatg
     3241 gcgcgtttgc aggaaagcgg tagccctatc gatctaatta ctcttgcgga atcgctggaa
     3301 cgccaggggc aactcgatag cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat
     3361 acgccaagtg cggcgaacat cagtgcctat gcggacatcg tgcgtgaacg tgccgttgtc
     3421 cgtgagatga tctcggttgc gaatgagatt gctgaagccg gttttgatcc gcaggggcgt
     3481 accagcgaag atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt
     3541 gcaaacaaag acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt
     3601 attgagcagt tgtttcagca gccacacgat ggcgttaccg gagtaaacac cggttatgac
     3661 gatctcaaca aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt cgccgcgcgt
     3721 ccgtcgatgg gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc gatgttgcag
     3781 gataaaccgg tacttatctt ctcgctggag atgccttcag aacagattat gatgcgttct
     3841 ctggcgtcgc tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct cgatgatgaa
     3901 gactgggcgc gcatttccgg caccatgggt attttgctcg aaaaacgcaa tatctatatc
     3961 gatgactctt ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt
     4021 gaacacggcg gcatcgggct tatcatgatc gactacctgc aactgatgcg cgtaccggcg
     4081 ctttccgata accgtacgct ggaaattgca gaaatctccc gctcgctgaa agcactggcg
     4141 aaagaactga acgtgccggt ggtggcgctg tcccagttga accgttctct ggaacaacgt
     4201 gccgacaaac gcccggtcaa ctccgacctg cgtgaatctg gctctatcga gcaggatgcg
     4261 gacttgatca tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc
     4321 atcgcggaaa ttattatcgg taaacaacgt aacggcccaa tcgggacggt acgcctgacc
     4381 tttaacggtc aatggtcgcg cttcgacaac tatgcggggc cgcagtacga cgacgaataa
     4441 taattatttt atgaattagg taattaaagc aaacacttat caaggaacac aaatgcaagc
     4501 ggcaactgtt gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact
     4561 ggcccctgcc agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc acggtcttct
     4621 tgagaccgcg cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc
     4681 tctgcgactg cgtgcggggg gaatcaccaa acctgtactg ttactcgaag gcttttttga
     4741 tgccagagat ctgccgacga tttctgcgca acattttcat accgccgtgc ataacgaaga
     4801 acagctggct gcgctggaag aggctagcct ggacgagccg gttaccgtct ggatgaaact
     4861 cgataccggt atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt tttatcatcg
     4921 cctgacccag tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc
     4981 ggatgaacca aaatgtggcg caaccgagaa acaactcgct atctttaata ccttttgcga
     5041 aggcaaacct ggtcaacgtt ccattgccgc atcgggtggc attctgctgt ggccacagtc
     5101 gcattttgac tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc tggaagatcg
     5161 ctccaccggt gccgattttg gctgtcagcc agtgatgtca ctaacctcca gcctgattgc
     5221 cgtgcgtgag cataaagccg gagaacctgt tggttatggt ggaacctggg taagcgaacg
     5281 tgatacccgt cttggcgtag tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc
     5341 gtccggtacg ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat
     5401 ggatatgatc tgcgtagact taggtccaca ggcgcaggac aaagccgggg atccggtcat
     5461 tttatggggc gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag taagcgctta
     5521 cgaacttatt acgcgcctga cttcaagggt cgcgatgaaa tacgtggatt aatcgttctg
     5581 taatatttga ttgtctgtgc cggatgcggc gtgaatgcct tatccggcca ataaaatcct
     5641 aaaaattcaa taagttgatg ttctttcatg ctcttataaa ggtcgtgcct ctggcggatg
     5701 tacgtttgtc atgagtctca ctctgttgct aattgccgtt cgctcctgaa catccactcg
     5761 atcttcgcct tcttccggtt tattgtgttt taaccacctg cccgtaaacc tggagaacca
     5821 tcgcgtgttt caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg
     5881 ttttaaagaa gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga
     5941 agacggaatt attccacaac tgaaagccgt ggcggaggcg gaagcgcgcc tgaatgcgca
     6001 gcctcatggc gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat
     6061 tgcgccgctg ctgtttggtg cggaccatcc ggtactgaaa caacagcgcg tagcaaccat
     6121 tcaaaccctt ggcggctccg gggcattgaa agtgggcgcg gatttcctga aacgctactt
     6181 cccggaatca ggcgtctggg tcagcgatcc tacctgggaa aaccacgtag caatattcgc
     6241 cggggctgga ttcgaagtga gtacttaccc ctggtatgac gaagcgacta acggcgtgcg
     6301 ctttaatgac ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca
     6361 tccatgttgc cacaacccaa cgggtgccga tctcactaat gaccagtggg atgcggtgat
     6421 tgaaattctc aaagcccgcg agcttattcc attcctcgat attgcctatc aaggatttgg
     6481 tgccggtatg gaagaggatg cctacgctat tcgcgccatt gccagcgctg gattacccgc
     6541 tctggtgagc aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact
     6601 ttctgttctg tgtgaagatg ccgaagccgc tggccgcgta ctggggcaat tgaaagcaac
     6661 agttcgccgc aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcagtgct
     6721 gaatgacgag gcattgaaag ccagctggct ggcggaagta gaagagatgc gtactcgcat
     6781 tctggcaatg cgtcaggaac tggtgaaggt attaagcaca gagatgccag aacgcaattt
     6841 cgattatctg cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt
     6901 tgaccgacta cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc
     6961 cgggttaaat acggcaaatg tacaacgtgt ggcaaaggcg tttgctgcgg tgatgtaatg
     7021 caggaaagca ggctggagct acccagcctg cagtgaaatt aaactgtcgt cgctttcact
     7081 ctttctttat agatgatttt tttgatgcca tcgttctacg tga
//
LOCUS       DAMZII010000198         7108 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZII010000198 DAMZII010000000
VERSION     DAMZII010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.198"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(102..545)
                     /locus_tag="POA77_004458"
     CDS             complement(102..545)
                     /locus_tag="POA77_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6367789.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            702..1631
                     /gene="metA"
                     /locus_tag="POA77_004459"
     CDS             702..1631
                     /gene="metA"
                     /locus_tag="POA77_004459"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDI6367790.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1900..3501
                     /gene="aceB"
                     /locus_tag="POA77_004460"
     CDS             1900..3501
                     /gene="aceB"
                     /locus_tag="POA77_004460"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDI6367791.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3531..4835
                     /gene="aceA"
                     /locus_tag="POA77_004461"
     CDS             3531..4835
                     /gene="aceA"
                     /locus_tag="POA77_004461"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDI6367792.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWKKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5116..6852
                     /gene="aceK"
                     /locus_tag="POA77_004462"
     CDS             5116..6852
                     /gene="aceK"
                     /locus_tag="POA77_004462"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDI6367793.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(6821..>7108)
                     /locus_tag="POA77_004463"
     CDS             complement(6821..>7108)
                     /locus_tag="POA77_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367794.1"
                     /translation="QQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELA
                     FELGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
BASE COUNT         1731 a         1842 c         1827 g         1708 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggtgaaa aaaatgccgg gtgcgacgct
       61 ggcgcgtctt ctccagcctg ggcaggcacg gcaggggcgg cttattcccc cacatacgcc
      121 agattcagca acggatacgg tttccccaaa tcgtccacct cagagcgtcc cgtaacctta
      181 aaacccacct tcttatagaa cccaaccgcc tgctcatttt gctcattaac gttggttgtc
      241 agttccggtg ccatcgagag cgcatgctcc accagcatcc gacccacgcc gcagccgcac
      301 acatcaggat cgataaacag cgcatccata tgctgcccac ttaacaacat aaacccaacc
      361 ggctgatccc gttcattaac cgcgacccac aacggtgctt ccggcagaaa ggaacgaacc
      421 agctcttcca gctcggcccg atactctgct gataaaaaat cgtgggtggc atcgacagat
      481 cgacaccaaa tcgcaacgag ttcctcccct tcctcatgcc gtgagcggcg aatactaata
      541 accattttct ctccttttag tcattcttat attctaacgt agtcttttcc ttgaaacttt
      601 ctcaccttca acatgcaggc tcgacattgg caaattttct ggttatcttc agctatctgg
      661 atgtctaaac gtataagcgt atgtagtgag gtaatcaggt tatgccgatt cgtgtgccgg
      721 acgagctacc cgccgtcaat ttcttgcgtg aagaaaacgt ctttgtgatg acaacttctc
      781 gtgcgtctgg tcaggaaatt cgtccactta aggttctgat ccttaacctg atgccgaaga
      841 agattgaaac tgaaaatcag tttctgcgcc tgctttcaaa ctcacctttg caggtcgata
      901 ttcagctgtt gcgcatcgat tcccgtgaat cgcgcaacac gcccgcagag catctgaaca
      961 acttctactg taactttgaa gatattcagg aacagaactt tgacggtctg attgtaaccg
     1021 gtgcgccgct gggcctggtg gagtttaatg atgtcgctta ctggccgcag atcaaacagg
     1081 tgctggagtg gtcgaaagat cacgtcacct cgacgctgtt tgtctgctgg gcggtacagg
     1141 ccgcgctcaa tatcctctac ggcattccta agcaaactcg caccgacaaa ctctctggcg
     1201 tttacgagca tcatattctc catcctcatg cgcttctgac gcgtggcttt gatgattcat
     1261 tcctggcacc acattcgcgc tatgctgact ttccggcagc gttgattcgt gattacaccg
     1321 atctggaaat tctggcagag acggaagaag gggatgcata tctgtttgcc agcaaagata
     1381 agcgcattgc ctttgtgacg ggccatcccg aatatgatgc gcaaacgctg gcgcaggaat
     1441 atttccgcga tgtggaagcc ggactaggcc cggaagtacc gtataactat ttcccgcaca
     1501 atgatccgca aaatacaccg cgagcgagct ggcgtagtca cggtaatttg ctgtttacca
     1561 actggctcaa ctattacgtc tatcagatca cgccatacga tctacggcac atgaatccaa
     1621 cgctggatta atcttctgtg atagtcgatc gttaagcgat tcagcacctt acctcaggca
     1681 ccttcgggtg ccttttttat ttccgaaacg cacctcagga ggtgaataaa ttttattcat
     1741 attgttatca ataagttatc aagtattttt aattaaaatg gaaattgttt ttgattttgc
     1801 attttaaatg agtagtctta gttgtgctga acgaaaagcg cacaacgatc cttcgttcac
     1861 agtggggaag ttttcggatc catgacgagg agctgcacga tgactgaaca ggcaacaaca
     1921 accgatgaac tggctttcat aaggccgtat ggcgagcagg agaagcaaat tcttactgcc
     1981 gaagcggtag aatttctgac tgagctggtg acgcatttta cgccacaacg caataaactt
     2041 ctggcagcgc gcattcagca gcagcaagat attgataacg gaactttgcc tgattttatt
     2101 tcggaaacag cttccattcg cgatacggac tggaaaattc gtggtattcc cgcggactta
     2161 caagatcgtc gagtcgagat aactggcccg gttgagcgca agatggtgat caacgcactg
     2221 aacgccaatg tgaaagtctt tatggccgat ttcgaagatt cactggcacc agactggaac
     2281 aaagtgatcg acgggcaaat taacctgcgc gatgcggtta acggcaccat cagctatacc
     2341 aatgaagcag gcaaaattta tcagctcaag cccaatccag cggttttgat ttgtcgggta
     2401 cgcggtctgc acttgccgga aaaacatgtc acctggcgtg gtgaggcaat ccccggtagc
     2461 ctgtttgatt ttgcgctcta tttcttccac aactatcagg cactgttggc aaagggcagc
     2521 ggtccctatt tctatctgcc gaaaacccag tcctggcagg aagcggcctg gtggagcgaa
     2581 gtcttcagct atgcagaaga tcgctttaat ctgccgcgcg gcaccatcaa ggcgacgttg
     2641 ctgattgaaa cgctgcccgc cgtgttccag atggatgaaa tccttcacgc gctgcgtgac
     2701 catattgttg gtctgaactg cggtcgttgg gattacatct tcagctatat caaaacgttg
     2761 aaaaactatc ccgatcgcgt cctgccagac agacaggcag tgacgatgga taaaccattc
     2821 ctgaatgctt actcacgcct gttgattaaa acctgccata aacgcggtgc ttttgcgatg
     2881 ggcggcatgg cagcgtttat tccgagcaaa gatgaagagc gcaataacca ggtgctcaac
     2941 aaagtaaaag cggataaagc gctggaagcc aataacggtc acgatggcac atggattgct
     3001 cacccaggcc ttgcggatac ggcaatggcg gtattcaacg acattctcgg ctcccgtaaa
     3061 aatcagcttg aagtgatgcg cgaacaagac gtgccgatta ctgccgatca gctgctggca
     3121 ccttgtgatg gtgaacgcac cgaagaaggt atgcgcgcca acattcgcgt ggcagtgcag
     3181 tacatcgaag catggatctc cggcaacggc tgcgtgccga tttatggcct gatggaagat
     3241 gcggcgacgg ctgaaatttc ccgtacctca atctggcagt ggatccatca tcaaaaaacg
     3301 ttgagcaatg gcaaaccggt gaccaaagcc ttgttccgcc agatgctggg tgaagagatg
     3361 aaagtcattg ccagcgaact gggcgaagaa cgtttctccc aggggcgttt tgacgatgcc
     3421 gcacgcttga tggaacagat caccacttcc gatgagttaa ttgatttcct gaccctgcca
     3481 ggctaccgcc tgttagcgta aaccaccaca taactatgga gcatctgcac atgaaaaccc
     3541 gtacacaaca aattgaagaa ttacagaaag agtggactca accgcgttgg gaaggcatta
     3601 ctcgcccata cagcgcggaa gatgtggtga aattacgcgg ttcagtcaat cctgaatgca
     3661 ccctggcgca actgggcgcg gcgaaaatgt ggcgtctgct gcatggcgag tcgaaaaaag
     3721 gctacatcaa cagcctcggt gcactgactg gcggtcaggc gctgcaacag gcgaaagcgg
     3781 gtattgaagc agtctatctg tcgggatggc aggtagcggc ggacgctaac ctggcggcca
     3841 gcatgtatcc ggatcagtcg ctctatccgg caaactcggt gccagctgtg gtggagcgga
     3901 tcaacaacac cttccgtcgt gccgatcaga tccaatggtc cgcaggcatt gagccgggcg
     3961 atccgcgcta tgtcgattac ttcctgccga tcgttgccga tgcggaagcc ggttttggcg
     4021 gtgtcctgaa tgcctttgaa ctgatgaaag cgatgattga agccggtgca gcggcagttc
     4081 acttcgaaga tcagctggcg tcagtgaaga aatgcggtca catgggcggc aaagttttgg
     4141 tgccaactca ggaagctatt cagaaactgg tcgcggcgcg tcttgcagct gacgtgacgg
     4201 gcgttccaac cctgctggtt gctcgtaccg atgccgatgc ggcggatctg attacctccg
     4261 attgcgaccc gtatgacagt gaatttatta ctggcgagcg taccagtgaa ggcttcttcc
     4321 gtactcatgc gggcattgag caagcgatca gccgtggcct ggcgtatgcg ccatatgctg
     4381 acctggtctg gtgtgaaacc tccacgccgg atctggaact ggcgcgtcgc tttgcacaag
     4441 ctatccacgc gaaatatccg ggcaaactgc tggcttataa ctgctcgccg tcgttcaact
     4501 ggaagaaaaa cctcgacgac aaaactattg ccagcttcca gcagcagctg tcggatatgg
     4561 gctacaagtt ccagttcatc accctggcag gtatccacag catgtggttc aacatgtttg
     4621 acctggcaaa cgcctatgcc cagggcgagg gtatgaagca ctacgttgag aaagtgcagc
     4681 agccggaatt tgccgccgcg aaagatggct ataccttcgt atctcaccag caggaagtgg
     4741 gtacaggtta cttcgataaa gtgacgacta ttattcaggg cggcacgtct tcagtcaccg
     4801 cgctgaccgg ctccactgaa gaatcgcagt tctaagcaac aacaaccgtt gctgactgta
     4861 ggccggataa ggcgttcacg ccgcatccgg caatcggtgc acgatgcctg atgcgacgct
     4921 tgcgcgtctt atcatgccta cagccgttgc cgaacgtagg ccggataagg catttacgcc
     4981 gcatccggca atcggtgcac gatgcctgat gcgacgcttg cgcgtcttat catgcctaca
     5041 accgttgccg aacgtaggct ggataaggcg tttacgccgc atccggcaat tctctgctcc
     5101 tgatgagggc gctaaatgcc gcgtggcctg gaattactga ttgctcaaac cattttgcaa
     5161 ggcttcgacg ctcagtatgg tcgattcctc gaagtgactt ccggggcgca gcagcgtttc
     5221 gaacaggccg actggcatgc tgtccagcag gcgatgaaaa accgtatcca tctttacgat
     5281 catcacgttg gtctggtcgt ggagcaactg cgctgcatta ctaacggcca aagtacggac
     5341 gcggcatttt tactgcgcgt taaagagcat tacacgcggc tgttgccgga ttacccgcgc
     5401 ttcgagattg cggagagctt ttttaactcc gtgtactgtc ggttatttga ccaccgctcg
     5461 cttactcccg agcggctttt tatctttagc tctcagccag agcgccgctt tcgtaccatt
     5521 ccccgcccgc tggcgaaaga ctttcacccc gatcacggct gggaatctct actgatgcgc
     5581 gttatcagcg acctgccgct gcgcctgcgc tggcagaata aaagccgtga cattcattac
     5641 atcattcgcc atctgacgga aacgctgggg acagacaacc tcgcggaaag tcatttacag
     5701 gtggcgaacg aactgtttta ccgcaataaa gccgcctggc tggtgggcaa actgatcacg
     5761 ccttccggca cgttgccctt cttactgccg atccaccaga cggacgacgg cgagttattt
     5821 attgatacct gcctgacgac gaccgccgaa gcgagcattg tttttggctt tgcgcgttct
     5881 tattttatgg tttatgcacc gctgcccgca gcgctggtcg agtggctacg ggaaattctg
     5941 ccaggtaaaa ccactgctga attgtatatg gctatcggct gccagaagca cgccaaaacc
     6001 gaaagctacc gcgaatatct cgtttatcta cagggctgta atgagcagtt cattgaagcg
     6061 ccgggtattc gtggaatggt gatgttggta tttacgctgc cgggctttga tcgggtattc
     6121 aaagtcatca aagacaagtt cgcgccgcag aaagagatgt ctgccgctca cgttcgtgcc
     6181 tgctatcaac tggtgaaaga gcacgaccgt gtgggccgaa tggcggacac ccaggagttt
     6241 gaaaactttg tgctggagaa gcggcatatt tccccggcat taatggaatt actgctccag
     6301 gaagcagcgg aaaaaatcac cgatcttggt gagcagattg taattcgcca tctctatatc
     6361 gagcggcgga tggtgccgct caatatctgg ctggaacaag tggaaggtca gcagttgcgc
     6421 gacgccattg aagaatacgg taacgctatt cgccagcttg ccgctgctaa catttttcct
     6481 ggcgacatgc tgtttaaaaa cttcggtgtc acccgtcacg ggcgtgtggt tttttatgat
     6541 tacgatgaaa tttgctacat gacggaagtg aatttccgcg acatcccgcc gccgcgctat
     6601 ccggaagacg aacttgccag cgaaccgtgg tacagcgtct cgccgggcga tgttttcccg
     6661 gaagagtttc gccactggct atgcgccgac ccgcgtattg gtccgctgtt tgaagagatg
     6721 cacgccgacc tgttccgcgc tgattactgg cgcgcactac aaaaccgcat acgtgaaggg
     6781 catgtggaag atgtttatgc gtatcggcgc aggcaaagat ttagcgtacg gtatggggag
     6841 atgctttttt gagtaaagct tccatataat ttttctccgc aatgtatcga gggttatccg
     6901 taaagccaaa gctttcagcc atcttattta atgtattaag gattaattca gcaataaccc
     6961 ggtgacccaa ttcaaaagcc aactcaaagg cagagtattt ttgtggggct ttgtgttgcc
     7021 aaaaatccat aatatcttca gcggtaaatc caaacaggcg tgcatggtca gataaagcaa
     7081 gataaaccgt ctctacaacg ttttgttg
//
LOCUS       DAMZII010000199         7070 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZII010000199 DAMZII010000000
VERSION     DAMZII010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.200"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            99..1433
                     /gene="adeQ"
                     /locus_tag="POA77_004464"
                     /gene_synonym="yicO"
     CDS             99..1433
                     /gene="adeQ"
                     /locus_tag="POA77_004464"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDI6367795.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1486..1938
                     /locus_tag="POA77_004465"
     CDS             1486..1938
                     /locus_tag="POA77_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDI6367796.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2149..3339
                     /gene="nepI"
                     /locus_tag="POA77_004466"
     CDS             2149..3339
                     /gene="nepI"
                     /locus_tag="POA77_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDI6367797.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3380..3673)
                     /locus_tag="POA77_004467"
     CDS             complement(3380..3673)
                     /locus_tag="POA77_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367798.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3895..4713
                     /gene="nlpA"
                     /locus_tag="POA77_004468"
     CDS             3895..4713
                     /gene="nlpA"
                     /locus_tag="POA77_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDI6367799.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4717..5640)
                     /locus_tag="POA77_004469"
     CDS             complement(4717..5640)
                     /locus_tag="POA77_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6367800.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5751..6935)
                     /locus_tag="POA77_004470"
     CDS             complement(5751..6935)
                     /locus_tag="POA77_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI6367801.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSRFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSSHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1771 a         1598 c         1788 g         1913 t
ORIGIN      
        1 cattaatgtg caattgaaat gtgataatta tcactgaacc gttgttgtca tttgttttac
       61 aaaagcaagg gattaaatct cctccaatgg acaaaaaaat gaataatgac aataccgatt
      121 acgtgagtaa tgaatcaggg acgctttcgc gattatttaa actacctcag catgggacca
      181 ccgtccgcac agaattgatt gcggggatga ccactttttt aaccatggtg tacatcgttt
      241 ttgtgaaccc gcaaatcctc ggcgcggcac aaatggaccc gaaagtggtg tttgttacca
      301 cctgtttgat tgccggtatc ggcagtattg cgatggggat atttgctaac ttacccgtgg
      361 cgctggctcc ggcaatgggg ctgaacgcct tctttgcctt cgtggtcgtg ggggcgatgg
      421 gcatctcctg gcagaccggg atgggcgcaa tattctgggg cgcagttgga ctatttttgc
      481 tcacgctgtt tcgtatccgg tactggatga tctccaacat tcccttaagt ttacgtattg
      541 gtatcaccag cggaattgga ttatttatcg ccttaatggg attaaaaaat actggcgtta
      601 ttgtcgccaa taaagacacg ctggtgatga ttggcgattt aagttctcac ggcgtgttgt
      661 taggtatttt agggtttttt attataaccg tgttgtcatc acgtcatttt catgccgcgg
      721 tgctggtttc tattgtggtg acgtcttgct gtggattatt tttcggtgat gttcatttta
      781 gcggcgtcta ttccattccg cctgatatta gcggcgtcat tggtgaagta gatttgagcg
      841 gcgcgttaac acttgaactc gccggtatca ttttctcctt tatgctgatc aacctatttg
      901 attcatcagg aacattaatt ggtgtaactg ataaagcggg cttaatagat agtaacggta
      961 aattccccaa tatgaataag gcgctgtatg ttgatagcgt cagttcggtg gcgggggcgt
     1021 ttatcggcac ctcgtctgtt actgcctata ttgaaagtac ttctggtgtg gcagtcggtg
     1081 gtcgcacggg gctgactgcg gttgtggttg gcgttatgtt cctgttagtt atgttcttct
     1141 caccgctggt ggcgatggtt cctccttacg caaccgccgg agcgttaatc tttgttggcg
     1201 tgctgatgac ttcgagcctg gcgcgcgtta actgggatga ttttaccgaa tcggtgcctg
     1261 cgtttattac cacggtgatg atgcccttta ctttctcgat caccgaaggg attgcactcg
     1321 gctttatgtc gtactgcatc atgaaagtat gcaccgggcg ctggcgcgat ctgaacctgt
     1381 gtgtggtggt ggtcgcagct ctgtttgcac tgaagattat tctggtggat tagtaactgg
     1441 cgagggtttc cagatatcat gagttctgat tacgcaggag aactcatgat ctggataatg
     1501 ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg tgctgacatc cggggccaga
     1561 aaagcgattc gccgtctcag cgatcggctg aacatcgatg tcgtacccgt ggagtcgatg
     1621 gtcgatcaaa tgggaaagtc agctggtgac gaatttttac gttatttgca tcgtccggat
     1681 gagtcgcacc tgcaaaacgc cgcgcaggtg ttgctcatct ggcaaattgt cattgtcgat
     1741 ggtagcgaac agaacctgct gcaatggcat cggattttac aaaaagctcg cctcgccgcg
     1801 ccgattaccg acgctcaggt caggctggcg ctaggttttc tgcgcgaaac cgaacctgaa
     1861 atgcaggata ttaatgcttt tcagatgcgc tataacgcgt tctttcagcc tgccgagggc
     1921 gttcactggt tgcattgaaa ccgtttcgtc ttatcagcct acagaccgcg cctgacctgt
     1981 cgggtggata agacgttcac gccgcatccg gtaatctttg ccctgttccg tttcgatctt
     2041 aactccagct caagcaacac cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca
     2101 atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt
     2161 attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg
     2221 gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt tgcccgtcag tttgttgacg
     2281 ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc
     2341 gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc tactgaccgc
     2401 cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt
     2461 gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg
     2521 ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag
     2581 gcactgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc
     2641 agttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggtgatgggc
     2701 gtgctgtgta ttttctggat tatcaaatca ttgccttcac tgccaggcga accctcgcat
     2761 cagaaacaaa atactttccg cttattacaa cgtccgggtg tgatggcagg gatgatcgcc
     2821 atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt atattcgccc ggtgtatatg
     2881 aacctggcgg gattcggcgt ggatggctta acgctggtgc tgttgagttt tggtatcgcc
     2941 agctttattg gtacgtcgct ttcgtcgttc attcttaaac gttcggtaaa actggcctta
     3001 gcaggcgcgc cgttaatact ggctgtgagt gcgttggtgc tgacgttgtg gggaagcgat
     3061 aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa cttttgcatt ggttcccgtc
     3121 ggctggtcaa cgtggatcac ccgctcgctg gccgatcagg cagaaaaagc cgggtctatt
     3181 caggtggcgg ttattcagct tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg
     3241 gataatattg gtctgacttc gccgttgatg ttgtccggca cattgatgtt gctgactgca
     3301 ttgttggtta ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg
     3361 tcagtagttg ttgctgttct tacatatccg ggcattctac cttacccaac gcttcccaat
     3421 aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt
     3481 tttcatctga caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc
     3541 aaattttacg taaatcttgt aataccgtgg ttttctcttt tgcggactgc agtgagctaa
     3601 aaggcgactc ggcgtatacg atgcctggca tagcgaaaaa agtgaaaata agcagtagtg
     3661 tcgttggttt catggattca accttttaca aatgtgtatc gaatgaagca taagtgtagt
     3721 ttgctttcat cgctctcatc atgctgtacg aaacgcgctt gcgtggtcag taagaagtgc
     3781 cagactttat attccacttt tattcctttt tattcttatc gatagcgttt cgttttttaa
     3841 accgcagcga ccttaccgct atagtcaggt aatcattaat aaaaggataa aaaaatgaaa
     3901 ctgacaacac atcatctacg gacaggggcc gcattattgc tggccggaat tctgctggca
     3961 ggttgcgacc agagtagcag cgatgcaaaa cacattaaag ttggcgttat aaatggcgca
     4021 gaacaagatg tcgcggaagt cgctaaaaag gtggcaaaag agaaatatgg cctcgatgtt
     4081 gaactggtag gatttagcgg ttcgttgcta ccgaacgatg cgacaaatca tggcgaactt
     4141 gatgcgaatg tcttccagca tcgacccttt cttgaacaag ataatcaggc gcatggttat
     4201 aaactggtag cggtgggaaa tacgtttgtc ttccctatgg cgggttattc caaaaaaatc
     4261 aaaaccgtgg cgcaaataaa agaaggcgcg acagtggcga tccccaacga cccgaccaac
     4321 cttggtcgcg cacttttact gttgcaaaaa gagaagctaa ttactctgaa agagggaaaa
     4381 gggttattac ctaccgcgct ggatataacc gataatcctc gtcatttgca gattatggaa
     4441 ctcgaggggg cgcagctccc gcgcgtactg gatgatccca aagttgatgt agcgattatc
     4501 agcaccactt acattcagca gacagggctt tctccggtgc acgacagcgt atttattgaa
     4561 gataagaatt cgccgtatgt gaatattttg gtggcacggg aagataataa gaatgcagaa
     4621 aacgtgaagg aatttctgca atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc
     4681 atttttaacg gtggcgcggt gcctggctgg taattctcac tcatgccgcg ccggacgatt
     4741 tatttttctg gcgcggcgac gggaatccat tgaaatcaaa atcaccgatg acagaatcag
     4801 cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc agcaacagca aagagagtag
     4861 cgcgctactt aatggttctg cacagctcaa aatgctggct tttggaccgc caattaattg
     4921 tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca aataaaaaaa
     4981 cgccagaatc aaactgccgt taacgacaaa gtttgttcct tgtctggcat aaaaaggcaa
     5041 cagaatcaga ccgccaatca gcatactcca gccgacgact ggtaacgtgc catagcgggc
     5101 aattagcgtt gagggatagg tggtatagaa tgcagcagca aatgccgagg caatgcccca
     5161 gaacaacgcg gcaggagaga tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa
     5221 agtcccgacc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc gcgatttacg
     5281 caccagtgag aaccaggcga cgataatcgt cggtgagagg aattgcagca ccgttgccgt
     5341 ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacagtta gcgcgccaac
     5401 cacggaaaaa atcagcaggc taatggcatc tttatgattg ttaataatag aaaagatttt
     5461 atcgccatga acaaatgaca gcgtcagtag aatcaaaccg gcgaatatca aacgcgtcat
     5521 agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc agacccctga
     5581 acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg tggaacccat
     5641 ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga
     5701 ggatggctct gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct
     5761 ttaataaaca gcaggcaaat catcgcaata cccaacatcc ctatcgccag ccagaacaac
     5821 gcatggtagc tccagatttc aaccataatt ccgtcaacag agccggcgat aatccagccg
     5881 acgcgtgaag tatttgcata taacgttgtc gcagagccta ttttttcagg catcaagtcc
     5941 tgaaaataaa gcatgccgat accacagaga ataccaagga agatggcatt aagaatttgc
     6001 aattccagct caaccgccgg agtcgtcgcc atgagtacgc tggcgtaaaa acacattcca
     6061 ctcacgatag caatgagcat taatagtcgc ttgccaatac gtttcatgta atagcctgcg
     6121 attaacatca tcggaatttc cagaccggca gcgataccaa tcatttctcc agccagttta
     6181 tcggttagat gcagttcatc aataataaat agcggcatat ttatcatgta gagattattc
     6241 gccgcccaca tcattgaaca gaccacaaaa agtagccgcg tatcccgctt cctgtgggag
     6301 gagggtaaaa tttctacggg ttgggtaacg acaggaatat ttctttgtat tgatggcaaa
     6361 aacaaccaga ctatcagtcc gcaaacaaca aatgcgatgg cagcggtgag atacatcact
     6421 ttaaaactaa atcccattgc cagttcataa gcgagcggtg gcccgataac ccaggcaagc
     6481 gagatctgag cacgtaaaaa tgtactgaac atgaccgtct cacggcctgt tctgtcggcg
     6541 tgttcacggg cgagggcgaa catttgcggg tttgcggtgg aagcaaaacg actcagaagt
     6601 acgcccgttg agaggagaat gaagtagttg cgattccacg caaaaagcgt gcaggccagc
     6661 actccaaata agcaacatag cagaatcagt aatttacggt cgccttgttt atcggagtgc
     6721 cttgccagaa attgactgac cagaattccc ataatagcgc taccggtgaa gaaaaaacct
     6781 accattatag gacgggcttt cagttcatct gcgaggaata tacttagggt aggagtctga
     6841 agagcgcccg caatacccgt cagaaaggcg acaagtaaaa atgccgcggc agtgagatca
     6901 agtatttttg atggagtggt agccgttttt tgcattcttc tgaatcaccg ggcgaaaaaa
     6961 tataactgag ttacaaataa gtaccagtcg aatgagtatt gtattaagaa tttttgagta
     7021 tcgctgatac aaccagaaag caatgctagc gcaatattat agggccagca
//
LOCUS       DAMZII010000200         7055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMZII010000200 DAMZII010000000
VERSION     DAMZII010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.201"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(195..680)
                     /gene="sixA"
                     /locus_tag="POA77_004471"
     CDS             complement(195..680)
                     /gene="sixA"
                     /locus_tag="POA77_004471"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDI6367802.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(886..3027)
                     /gene="fadJ"
                     /locus_tag="POA77_004472"
     CDS             complement(886..3027)
                     /gene="fadJ"
                     /locus_tag="POA77_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDI6367803.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="POA77_004473"
     CDS             complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="POA77_004473"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDI6367804.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERADLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4517..4801)
                     /locus_tag="POA77_004474"
     CDS             complement(4517..4801)
                     /locus_tag="POA77_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDI6367805.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5173..6513
                     /gene="fadL"
                     /locus_tag="POA77_004475"
     CDS             5173..6513
                     /gene="fadL"
                     /locus_tag="POA77_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDI6367806.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6879..>7055
                     /locus_tag="POA77_004476"
     CDS             6879..>7055
                     /locus_tag="POA77_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367807.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIR"
BASE COUNT         1799 a         1876 c         1652 g         1728 t
ORIGIN      
        1 acattattca tttcaacatg taactttggg caaaatcaag atacgcgcag aagaataatt
       61 tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga tagtagtgag
      121 gttgacagat atccgctgcc tgatgagcaa ttatgcccaa tgaatctacc tcattgggca
      181 taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct catctgccag
      241 ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac
      301 ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga
      361 gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc
      421 agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc
      481 agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg
      541 cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa
      601 ccattagtgg tcagaggacg aacggaatca ctggcggcat cgagggctgc gtcgccgtga
      661 cgcataataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc cgttttcttt
      721 catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg
      781 aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca
      841 acactccgcc attcagcgcg gattcatata gctttgacct tcttattaca ggtcagttgc
      901 agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt
      961 aaaacgggaa ccatactgcg tggcaagtcg ttgcattatt gcaaccactt cgcccgcgcc
     1021 gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac
     1081 cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc
     1141 ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg
     1201 cccttgtgcg ccaatgagcg ggtaaatggc gggatcgacc tgttttttgc ttttacgccc
     1261 tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa
     1321 aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac
     1381 aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttggacctac
     1441 cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac cttcggtcag
     1501 catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt
     1561 gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat
     1621 ggtttgcgct gatgtacccg catgaggaat aatctccacc agcggcattt tttccaccgg
     1681 actgaagaaa tgcaggccga taacttgctc aggtcgcgtg gcgtgagcgg cgatatcacc
     1741 aatcggtaaa gatgacgtat tcgaagcaaa gatggtatga gcggcgctat tttgctcaac
     1801 ttccgccacc atctgttgtt tcaattcgag attttcaaac accgcttcaa taatcagatc
     1861 gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct gtttgtcacg
     1921 ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt
     1981 cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt
     2041 acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct
     2101 gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacgtccg tactggcaaa
     2161 aaagatacta cgcagcgcct gcgattgtgg cgtcatcgcc agttcgccaa acgcccgagc
     2221 ttcggcgtca taaccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat
     2281 gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt
     2341 gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg
     2401 ggaagatggg cgatccttct tcgccagctc aacagcgact tccagcaaaa tggagtgcgg
     2461 aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt
     2521 gaggatcatc tctaatgctg tgctgacgcc tatcagacgc ggtaaacgct gggtgccgcc
     2581 tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc
     2641 agtacaaacg cgaccgtggc acgccagcgc caactccagc ccaccaccca ggcaagcgcc
     2701 atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc
     2761 ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccgatca tgttgatgtc
     2821 tgcgccagca atgaagttgt ccggtttagc ggagacaaac accacgcctc gcaactcttt
     2881 gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaactccg ccttcagggt
     2941 attcattttc tcacccggta cgtcgatggt gatgatagca atgttgtcca gacgaacatt
     3001 aagagtaaac actgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc
     3061 ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc
     3121 aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgcccgta agcaatcgaa
     3181 ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt
     3241 acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca
     3301 aaggcttcgt gcatatcgat caatgtcaga tcgctcattg tcaaatcggc acgttccagc
     3361 gccagcggtg ttgaccaggc cggaccgagc aacatgtcct gccagacatc aatcgcagta
     3421 aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca
     3481 gtcatcagga tcaccgccgc tgcgccatcg gtcagcggcg tactgttcgc cgccgttacc
     3541 gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta
     3601 ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc
     3661 acctcttctt tgagtttacc gtctgaccac gcctgagcgg cacgctgatg cgaacggtgc
     3721 gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg
     3781 tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg
     3841 cgcaaacgca ggcgagagaa gagtttcagt cgctggctca tggttcgagc tttgttgaca
     3901 tcaaccagca cgcgcgccag ttttttactg acgccaattg gcaatacgga agaggaatct
     3961 gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca
     4021 accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc
     4081 attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc
     4141 tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg
     4201 cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt cgcctggcgg
     4261 gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagc
     4321 ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat
     4381 catagtctta accatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat
     4441 gacacagtga agacaggaga aagaaaagaa aacgcccctg ccagcaactg acaggggcga
     4501 acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg
     4561 caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga
     4621 ttttgcacgg ttcggattcc acgctacggg ctttttcagt cagcgccgcc agcgtttgtt
     4681 ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca
     4741 taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca
     4801 tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg
     4861 cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca
     4921 cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt
     4981 ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcaa
     5041 cattccagct ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag
     5101 accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt
     5161 gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg
     5221 gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct
     5281 ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc
     5341 cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat
     5401 attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat
     5461 aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa
     5521 tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact
     5581 tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc
     5641 ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc cgtctacgct
     5701 cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg
     5761 caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc
     5821 gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc
     5881 ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg
     5941 aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt
     6001 ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg
     6061 cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat
     6121 agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc
     6181 gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc
     6241 acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca
     6301 gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca
     6361 ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac
     6421 ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg
     6481 ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc
     6541 tgcatagcag gtgactttaa ctcccccact tcaccgaaag tagtgtcctc attgcttacc
     6601 tcctgagttt tgcaaacagc ctgttggcag cttgcccttt caaatcaata agcggtatcc
     6661 acagacaaac ctgaagggaa aggcatattt tcaggcgttc tgctcgtcct tctcaaagag
     6721 tttacttttc tgcatttcca ggatactcaa cccccctggc tattgtgcgc tcatacactc
     6781 aaattaaaga taggttctaa ataaatgagc gttttttgat agtctatttc attaggtaat
     6841 atatatttgt aacaaatcaa tcaaaatgga ataaaatcat gctaccatct atttcaatca
     6901 acaataccag cgcaacttac ccagaatcca ttaatgaaaa taacaatgat gaagttaatg
     6961 gattagtaca agagctcaaa aaccttttta atggtaagga aggaataagc acctgtatta
     7021 aacatctact tgagcttata aaaaacgcca tacga
//
LOCUS       DAMZII010000201         7033 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZII010000201 DAMZII010000000
VERSION     DAMZII010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.202"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            28..933
                     /locus_tag="POA77_004477"
     CDS             28..933
                     /locus_tag="POA77_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6367808.1"
                     /translation="MLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDID
                     AQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAK
                     AYALFNQAAQHGGNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATC
                     RKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSI
                     RRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTT
                     IVAATATINGKDVELLKAGAIEQKW"
     gene            complement(989..1531)
                     /locus_tag="POA77_004478"
     CDS             complement(989..1531)
                     /locus_tag="POA77_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6367809.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1515..1826)
                     /locus_tag="POA77_004479"
     CDS             complement(1515..1826)
                     /locus_tag="POA77_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI6367810.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2011..2901)
                     /gene="cyoE"
                     /locus_tag="POA77_004480"
     CDS             complement(2011..2901)
                     /gene="cyoE"
                     /locus_tag="POA77_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI6367811.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2913..3242)
                     /locus_tag="POA77_004481"
     CDS             complement(2913..3242)
                     /locus_tag="POA77_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDI6367812.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3242..3856)
                     /locus_tag="POA77_004482"
     CDS             complement(3242..3856)
                     /locus_tag="POA77_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDI6367813.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTITNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3846..5837)
                     /gene="cyoB"
                     /locus_tag="POA77_004483"
     CDS             complement(3846..5837)
                     /gene="cyoB"
                     /locus_tag="POA77_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDI6367814.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5859..6806)
                     /gene="cyoA"
                     /locus_tag="POA77_004484"
     CDS             complement(5859..6806)
                     /gene="cyoA"
                     /locus_tag="POA77_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDI6367815.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1912 a         1758 c         1751 g         1612 t
ORIGIN      
        1 ttaactaaaa gaatattatt ttctgtaatg ctgatagttt caccaagcgt ggtggccagc
       61 gagaaagcgc atgaattgta tgacagcata tacggtggta aacctgctcc cgatgttata
      121 aatacactgc ataaaatggc tgaatcggga gatatcgacg cgcagagcct gttaggctgg
      181 gaatattatc agcctcgtta tgataccaaa cctgatgttc aggaagcgat taaatggttc
      241 gagttagcgg ctaaacaagg tgacagagaa gctccgttag cgctgggggg tatctactac
      301 gacggtgagc aggtgcgggt ggattatgcc aaagcgtatg cactatttaa tcaggccgcg
      361 cagcacggtg ggaatttagc atggtccagg ttgggcatta tgtacgccaa tggtcagtat
      421 gttgaggtag attgtaagaa agcgaaggaa tatcttgata aaggtgtcca catttatggt
      481 ggcccagaag actttctggc tacttgtcga aaagacatga ttgacagaaa aacggttgac
      541 gatacgttac ccgtgattac ggttacccgt tctgggatgc gagataattt tttagataaa
      601 ggtttttcct gcatggatag cctcttcgcc accaccaata aattaggcga agtggccaat
      661 cttcgcgtga cattcagcat tcgtcgccca tccggtaagg agattaacca aacggttggc
      721 ttcgcgcctt tcggtttaaa ccggctgaat attagcttta ctgattacct atttggttca
      781 tttaccagta attcgtctct tattctttat aaaccggaat ttgagcgaaa atcttgtgca
      841 actgtaagga ccacaatcgt tgccgcaacg gcaaccatta acggcaagga tgtggagttg
      901 ctgaaagcgg gggcaattga acaaaagtgg taacgctgct cagcccccgg ctgaggcaaa
      961 acttgccctg gcgggtggcg ataataatct aagccgcgag aatatgctga atcgccaaaa
     1021 acataggtac agcaccaaaa gcatttggtg tcgctgaaag ctgaacaaag ccgagacgag
     1081 cataaaaatt aatggcggca gggtcagcat caagataaac ccctttaatt ggtaatgcct
     1141 ggtgaattat ttttacatgt tcaaaaaaat cacatagtag gtcctgacca aagccccgct
     1201 tttgatactt ccgtgctacc cccaacatga ctaatctgac aacaccaatt tctgaaggtt
     1261 gtgaaccctg aaggacgcca gaaacgcgtt gtttttccag cgaatatgcc gtaaaagtac
     1321 agatgccgat cagttcacca ctttgtctgt caataagtgc tttagccgca cagtcgctat
     1381 tacgcacact tttctttagc gatgcgcgta caaatttatc gataacaggg ttaccgcaat
     1441 caaactgttt ttgcccagga taagtaatat cagactgata attacaggag aggactatgg
     1501 gcagagtaat ctcttcatgc ttgtcgacca tcgctgtttt tccttttcat taacgccttg
     1561 agggcgagcg tcggttgagc aggttcagcg atgagttggt tcatcagttc ccagctttga
     1621 ttcgaaagct cacggcgacg ttggttatca agcacacttt cggcgcgttc catagcagcg
     1681 ctcaaaataa aagcgcttaa atctacccca acggcttgcg cagcttcacg aagtttgttt
     1741 ttggcgtcgg gactagtttt gagttcgacc cgcgcatttt tcagcgtttt atcgacatcg
     1801 ggtagagtgg ctgtttttcc aggcattatg acctctttga ttaactgaat gaaatcgtag
     1861 ggagtcgatc tcatacataa tacggctatc aaccgtatga ttaatttatg ttcatctgct
     1921 cactctgtca agatatacgg ttattagccg tatctctgat ctcagacaat aaaaaaggtg
     1981 ccgtagcacc tttttaatag agaggttttg ttaccacaca gcagccagca gcgtatgcga
     2041 gtccggtacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg cgatgataga
     2101 gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac cgcgcagagc
     2161 catacctaac caccagacgc taaccgccgc ggcgaccacc agatatttat atccagcgta
     2221 accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg
     2281 attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg ccgcctggta
     2341 atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca ggctgaagat
     2401 agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt agccaatcac
     2461 cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagactgagt ggcgtttcat
     2521 gtacaggcta tagacgccga cataaaccac aaagcccatc acccccagcc agcaggccag
     2581 cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg tggcgtacac
     2641 cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg tcctttccat
     2701 ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg ccacaaccag
     2761 tgacacccca accagcgtgt agataaacag gggataatca atgctgccct ttgaggccag
     2821 caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt tcgttacttg
     2881 caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt gtagttgagg
     2941 ttccacataa tccagatgga gcctacaacc aggatagcga tgattagcac ggtgaagaca
     3001 aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa gcacaccaga
     3061 tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac ggccggagag
     3121 gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga caggataaag
     3181 cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt agaatgactc
     3241 attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc acatccagga
     3301 agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgatggtc aggccgcgac
     3361 gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg tgcagaccgt
     3421 gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccgga cccatgccgt
     3481 taacaatcag gtgatggaat tcgtagattt ccatcccgat aaatccggca ccaaacaacc
     3541 aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg gcgatagccg
     3601 ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca
     3661 gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg
     3721 tagcaaacaa gattgagaac agaatgcagt cgctcatcag gtagatccaa aatccgaaga
     3781 ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtgggcagtc gcgtgcgtca
     3841 aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg ctggttttcc
     3901 agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa gcttttcacg
     3961 atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat atgccagatc
     4021 atggcgaaac cgaagatggt ggagaaagct gcaatgacga tacctgcacc gctgtttttc
     4081 ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt ctctttcatt
     4141 tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata gaacggaggc
     4201 ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg
     4261 ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa cgaggcagag aatacccagc
     4321 gcaatcagta ctgcaccgct agctgcaatc atcagcatgg tgtggaactg cgggtcaatc
     4381 tgctggctca aacgacgggt catgcccatg aagcccagcg catacagcgg cataaaggca
     4441 acgaagaagc cgatgatcca gaaccagaac gcgcgtttac cccaggtttc attcagtttg
     4501 aaaccgaagg ctttaggcca ccagtaggtc atccctgcga agcagccgaa gaccacgccg
     4561 ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc
     4621 gcgcccggta ctgccagcag cacgccagtc atcccgccca ccgagaaagt gacgataaaa
     4681 ccgatggtcc acagcatcgc agaatggaac acgatgcggc cctgatacat ggtgaacagc
     4741 cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat accaaagaag
     4801 gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac gatgaacgac
     4861 agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag acgtttacgc
     4921 gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag gatgtaaact
     4981 tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc acccatatcg
     5041 ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac ggtaaccgtc
     5101 agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact
     5161 ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac gaagaagttg
     5221 ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca gtaatcgaca
     5281 ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca gccggtctgc
     5341 gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac agtaaaccag
     5401 aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccgatctg cagcggaacc
     5461 accaggttca tcagaccgat aacgaaaggc atcgccacga agaagatcat aatcacgccg
     5521 tgcgcggtga agatctgatc gtagtggtga ggtggcagga agcccgcttc gcccgccgag
     5581 gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag caacatcaca
     5641 atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt cagccactct
     5701 ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc cagacctccc
     5761 aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac tgcatcaagt
     5821 gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga ttccgcgtgg
     5881 ctcatgtcca tgccttccat accttcgtgt gcactgtgct caccttctgg ctgggtcatg
     5941 tccatgctct taccgtgagc cataaacttg ttaatcacat cggcaaacaa gtctggtttc
     6001 acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt ttcgaacgca
     6061 gccatgtcag acatggtgtt cggcgactgt ttcgcttttg cgacccattg gtcgaattcg
     6121 gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg cccgctgtag
     6181 ctggcggata taccgtcata agtaccgggt tcgttggcga tcagatgcag gcgagtctgc
     6241 ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga gttcatcacg
     6301 gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg
     6361 gtagcaatgc cctgttccgg gtagatgaag aaccatttcc agtccatgga aaccacttcg
     6421 atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt
     6481 ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca gaccacagct
     6541 tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt gctcgcacga
     6601 tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat caacatcagg
     6661 ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt gggatctaac
     6721 agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa
     6781 cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat ctggtggcgt
     6841 ttaaagtgtg tgggcatttt acgggaaggt tacattactg taaacatgat taaattcctg
     6901 ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaagtt gattttaaga
     6961 agctgttgca aaagggcgtt ataacgaaag gggagtgcag aaggagtttg gacgggcaat
     7021 tggtataacc aat
//
LOCUS       DAMZII010000202         7014 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZII010000202 DAMZII010000000
VERSION     DAMZII010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.203"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            52..1239
                     /locus_tag="POA77_004485"
     CDS             52..1239
                     /locus_tag="POA77_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDI6367816.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LLSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNTSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            1236..3176
                     /locus_tag="POA77_004486"
     CDS             1236..3176
                     /locus_tag="POA77_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HDI6367817.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            3180..4550
                     /locus_tag="POA77_004487"
     CDS             3180..4550
                     /locus_tag="POA77_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HDI6367818.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPTLKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            complement(4962..5659)
                     /locus_tag="POA77_004488"
                     /pseudo
     CDS             complement(4962..5659)
                     /locus_tag="POA77_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5801..5995
                     /gene="tnpA"
                     /locus_tag="POA77_004489"
                     /pseudo
     CDS             5801..5995
                     /gene="tnpA"
                     /locus_tag="POA77_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6124..>7014)
                     /locus_tag="POA77_004490"
     CDS             complement(6124..>7014)
                     /locus_tag="POA77_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367819.1"
                     /translation="VNDVYQYNNIPHALRVQIIKIITDSIGFPSSNERYTSYRNEADK
                     VYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEKCLDFIEICFQILVSHVA
                     KNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIRIDSQLIHADVVKPTLIL
                     LSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIMKAIHDKNNWKYSPNDTA
                     SKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAGHGQGADIKEVPEELASY
                     MLHLTATNLLFLLKCEKNIK"
BASE COUNT         1963 a         1601 c         1654 g         1796 t
ORIGIN      
        1 taactaagaa aacccagaat accactttca ttgttatgga aaacatggac aatgttaatg
       61 aaatatattt ctcaccgcgc catcatttgt accttgacac tgttgatcat tattatcacc
      121 gttttgttca cctttctgag aagctctgat gtgccagaat atattactgc cccggtgcgc
      181 aaaggcgata tagaaaattc tgttctggcg acaggccgta ttgatgcaat tgaacgggta
      241 aacgtagggg cacaggtatc agggcaatta aaatcactga aagtgaaaca gggcgaccat
      301 gtgactaaag ggcagttgat tgccgaaatt gacgacctac ctcagtgtaa cgatttgcgt
      361 aatgccgaag ctgcactcaa cgaagtgaaa gcagaacttc aatccaagca ggcgctcctg
      421 aaacaggctg aactgcgttt taaacgccag ctccggatgc taagagaaaa tgccagctca
      481 cacgaagatt ttgagtctgc ggaggcaatg cttgcgacca ctcgcgctga attactgtct
      541 ctgaatgcca aactggtcca ggcacagata gaagtagata agaaaaagct tgcccttgaa
      601 tatacgcgag tcgtagcgcc aatggatgga attgttattg ccattgtcac tcagcaaggg
      661 caaaccgtta actcaaacca gagtgcccca acaattatta aactcgctcg tctggatgtc
      721 atgaccatta aagcacagat ctccgaggct gatataaccc gtatttctgt ggggcaaaaa
      781 gcccgcttct ctatcttctc cgaaccggac aagcactaca gcgcaacact gcgtgccgtt
      841 gaactggcac cagaatcggt aatgaaagat gactctctcg ccagcaacac ttcagcatca
      901 ggctccggaa cttcaaatgc gtccgtttat tacaatgctc tattcgatgt gcctaaccca
      961 gaaaatcgac ttcgtattgc catgactgct caggttaccc tgattactga tgaagcccaa
     1021 aatacactgc tggtacccat tcaggcagtg cacagaaatg aggggaagaa gcaacaggtc
     1081 ctggtgctgg cagcagatgg caggctggaa ccccggaatg tgaaaactgg tatcacgaac
     1141 agcgtggata ttcagatcct cgaaggttta aatgtcggag aaaatgtcgt gctgtcgctg
     1201 ccggataaga aagagcccga agaaaggatt atgctgtgaa aaaacttatt gagttaaaag
     1261 gggttagccg aacttatggc aacggagatc aaacgcggac cgtcctgaaa aatgtcgatt
     1321 tgacaatcgt tgctggagaa atggtggcaa tcattggagc ctctggctca ggcaaatcaa
     1381 ccttaatgaa cataatgggt tgccttgacg tacccaatag aggtgattac tacatcgacg
     1441 gacaaaatgc cgcctgtctt tcacccgatg agctggctag agtgcgccgg gaacacattg
     1501 gctttatatt ccagcgctac catttgatac ctgatcttag cgcacttggt aacgtggaga
     1561 tcccggccat ttacgccaac agcgaacgcg acagccgtcg gcaacgtgca acggcgttgc
     1621 tgggcagact gggactggaa ggacgtgagc atcataaacc ttgcgaactt tcaggcggtc
     1681 agcagcaacg cgtcagtatt gcccgagcac tgatcaacgg tggaaagata attttggcgg
     1741 atgagccaac tggtgctctg gattcgcaat ccgggcaaga agtgttagcc attctgaatg
     1801 aacttaatcg acgtggtcat actgtagtaa tggtgaccca cgatatgaag gtcgcccggc
     1861 acgcaaagcg aatcattgag ctgtgtgatg gcgaaatcat cgctgatagt ggaggttgcg
     1921 tatctgcgac ggaaacacta ccaaaaacca acagaatccg gcaaagctat tggaaaacac
     1981 tgctcgatcg aacgcgtgaa tcgatgcaaa tggcgctaaa agcgatgaag acgcatcgct
     2041 tacgcaccac gcttaccatg atcgggattg tttttggtat cgcctccgtt gtcactgttg
     2101 tggcattggg tgaaggagcc aggcaagaaa cactggaaga gattaaaagt ctggggacaa
     2161 acgtggtcag catttatccc gggcaggatt tgttcgatga cagcattgag agtattagaa
     2221 cattggtacc tgctgacgca aatgcactgg cgaagcaagg gtttatcgat agcgtcagcc
     2281 cggaagttag cgcttcagac aacattcgtt ttctgggtaa atctgcgata gcgtccatta
     2341 acggcgttgg gcgggaacat ttccgggtaa aaggtattga gttgttgcaa gggactactt
     2401 ttagagacga tcgcaacgct ctacaggaag ttattatcga tgagaatact cgtaaggcca
     2461 ttttcgacaa cacagggtta caggctctgg ggcaaatagt ctttcttggt tctgtaccag
     2521 cacgagtggt tggcatcgct aaaagtaaca accgtagtga tgcatcaaat cgcattactg
     2581 tgtggatgcc ttacagcacg gtgatgtacc gtattgtggg taaaccagtt ctgaccggta
     2641 ttagcgtcag gctaaaagac aatgtggata acgaggctgc aatcagcgct atctcccaac
     2701 tattaacccg gcgtcacggc ataaaagact tccagcttta taactttgaa caaataagaa
     2761 agtctatcga acacacctca atgaccttca gtattttaat tcttatggtc gcatgtatct
     2821 cactaatgat cggcagtatc ggggtgatga acatcatgtt gatttctgta acggaacgaa
     2881 ctcatgaaat tggcgtacga atggcggttg gcgcgagacg cagcgacatt atgcaacagt
     2941 ttattattga ggccgtattg gtttgcctga ttggtggcgc actcggtatt gcactgtcgt
     3001 atatcacagg tgcgctcttt aacgctctgg cggacgggat attcgcagct atatattcgt
     3061 ggcaggctgc tgttgctgca tttttttgct caaccttaat cggcataatc ttcgggtatc
     3121 tcccagcccg caaggcagca aggatggacc cggttatttc actggccagc gaataatata
     3181 tgaaaatttt atcatcgttg actttatgcc ttatcaccgc actctgttca ggttgcacaa
     3241 acgtgctgaa aagtgactat cgcgctcccg aagtgaacta tcccatcaac tggaccaagg
     3301 gcgatgtgga cggaaataca tccccgtttg actgggaaga atttaacgat cctaatctcg
     3361 ataactggct tcacctggtg atgacaagta ataacgacat agctatagct gcactgcgga
     3421 tacatcgggc gcagctggat gcggaaagaa caggtatcac caatactcct acactaaagg
     3481 cggcactgag tatggacgga aaaaaacagc tgaataactc gtccggctgg gcaaaaagcg
     3541 gttctgccag ccttggcacc agctatgagc tggatctctg gggaaaaatt gcccgtcagc
     3601 gtgatgttgc tgagtgggca gtccatgcca gtgaagaaga ttttcgctcc gcgcgcctga
     3661 tgctgctttc agaggccagc aataattact ggcgtatcgg ttttgtaaat caacaaatta
     3721 ccaccctcca gcagagtatt gactatgcaa aagagacgct gcgtctggcc gaggttcgct
     3781 atcgtgcagg aaatatctcg tcgctggatg tgattgacgc gcagcaaaac ctgctcacgc
     3841 aagagaacca gcttacaggg ctacaacgag aacattcaca gctactcaat cagcaggccg
     3901 ttttgctcgg caccgtgccc ggttgccaga tagtcgagcc cacaacgctg ccaaaaggaa
     3961 gcctgcctaa ggtcaatgcg aatattcctg ccagcatact gatgcgccgg cctgacataa
     4021 gtgctaaaga gtggcagttg cgtgaggcgc tagctacggt tgatatcaag cgtagcgaat
     4081 attaccccac cttcaacctg acaggcgctt tgggtaccag cagcgcttcg ctactggcat
     4141 tactgcataa tccggtcggt tccgtaggcg ctaacctgac gctaccgttc ctggagtggc
     4201 gacagcggga catagaggta aaaattgccc gtaacgatta cgaacagcga gtgctggagt
     4261 ttaaacagct actttataag gcaatgagca gtatagagga cgcgctttca tttcgtaacc
     4321 agttgctgct tcaggagaca agactcaggg aagaactgga acttgcacgt aagtcagagt
     4381 ggttaaacga agttcgttac cggcatggtg cagtacgtat ttcattctgg cttgatgctc
     4441 aggaaaaacg tcgtcaggcg gagctgcgac tggatgaaaa ccgcttcaat cagttgcaga
     4501 atctagcaaa aatttatctt gagtttggcg gggcatcaac ctttccttaa gtctaattgg
     4561 ggacaaccag gagcaataag gagtgaaaaa tagccacagc tgaaaacacc atagccattg
     4621 acatttgagc tttacctgct cttgaaaaca aaaaatcact gttaagcgca atgctattca
     4681 cttcagcaga gcagtattgc gtgtcacgag catgtttacc attctgtgta aatatctttt
     4741 cttaaaagtg atcatcaaag cagtcatcaa actcgataaa tatccaccgg catagcgtaa
     4801 tgccgagcag ttaaggatca attgaccgct ccttaaactg cggcactata acggcttcta
     4861 caacagggag ccgttttctt gtgcatagaa aacccccagc taggctgggg gttccggaaa
     4921 gctttcagct ttgagccagt tattaaggta atgactccaa cttactgata gtgttttatg
     4981 ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagtgac
     5041 ttcctgccca gccttgccag atgctgcctc agattcaggt tatgccgctc aatgcgctgc
     5101 ctatatcgct tgctgataac gtgcagttct cccttcaggc gtgattcata cagcggccag
     5161 ccatccgtca tccataccac gacctcaaag gccgacagca ggctcagaag acgctccagc
     5221 gtggccaacg tgcgttcacc gaatacatgc gccacaaccg tcctccgtat cctgtcatac
     5281 gcgtaaaaca accagcgctg gcgtgattta gcgccgacgt aaccccactg ttcgtccatt
     5341 tccgcgcaaa caatgacgtc actgcccggt tgtatgcgtg agtttaccga ctgcggcctg
     5401 agttttttaa gtgtcgtaaa atcgtgttga ggccaacgcc cataatgcgt gcactggcgc
     5461 gacatccgac gccattcatg gccatatcaa taattttctg gtgtgtaccg ggctgagagg
     5521 cggtgtaagt gaactgtagc tgccatgttt tacggcagtg agagcagaga tagcgctgat
     5581 gtccggcagt acttttaccg ttacgcacca cgccttcagt agctgagcag gagggacaac
     5641 tgatggagat ggaagccacg ggagcacctc aaaaacacca tcatacacta aatcagtaag
     5701 ttggcaccat taccagttat taaaacccct tttgatttgt taaaacacct tgcggtctgg
     5761 caactgcaag tgtcaaacaa gaaatcaaaa gggggtccca atggggaacg aaaagagctt
     5821 agcgcacacc cgatggaact gtaaatatca catagtattt gcgccaaaat accgaagaca
     5881 ggtgttctac agagagaagc gtagagcaat aggctgtatt ttgagaaagc tgtgtaagtg
     5941 gaaaagtgta cggattctgg aagctgaatg ctgtgcaaat catatacata tgctacaatg
     6001 tttgtcaaag ctcagtgaaa atgtctaatc actggctcat tataaatgtc catatattga
     6061 aaaggccgct gcgaaaatcc ctcccgcagc ggcctcttta gcataacgtc attatgcgaa
     6121 ccatcactta atgttcttct cgcattttaa gagaaatagt aaatttgtag cagtaagatg
     6181 aagcatatag cttgccaatt cttccggaac ctctttaata tcagctcctt gcccatgacc
     6241 tgcatttttg tttctgatgg ttggaattcc tgtttccagc attgttttca gacttgtgaa
     6301 ctggctttgt aaatatgccg gaattaaatt ttgacttaaa cagctattta ttagttttga
     6361 tgctgtatca ttaggactat atttccagtt attcttatca tgtattgctt tcattatact
     6421 ttcaaatgat ttcagacaat catttagaca ttctttataa cgcttatgcc tgtaatgctc
     6481 atgcgcagca aggaattcat cattggcgcc ttcaaataag ggttctccac ttaaaagaat
     6541 cagggttggc tttacgacat cagcatgaat caattgagag tctatcctta ttatctcttc
     6601 agattcaaac tgataaccaa ccccatgttc tctaaacctt tcatttagtt caataactgc
     6661 atctccagga ctttgacttg ttatgtcttt gaattcataa tggtttttcg caacgtggct
     6721 cactagtatc tggaagcata tttctatgaa atctaggcat ttctctgtat ttctctcttt
     6781 aaggaaaaag tcgacaagag cgtcaaagtc attctttgca aattccttca aagaaaaaac
     6841 accatattcc ttagaaagta tctcatggat atatgcatat actttgtcag cttcatttct
     6901 atacgatgtg tagcgttcat tagaagaggg gaagccgata gaatcagtta ttattttaat
     6961 tatttgcacc ctgagtgcat gtggaatatt gttatattga tatacgtcat ttac
//
LOCUS       DAMZII010000203         6977 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZII010000203 DAMZII010000000
VERSION     DAMZII010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.204"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(54..845)
                     /gene="aadA1"
                     /locus_tag="POA77_004491"
     CDS             complement(54..845)
                     /gene="aadA1"
                     /locus_tag="POA77_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDI6367820.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            994..2007
                     /gene="intI1"
                     /locus_tag="POA77_004492"
     CDS             994..2007
                     /gene="intI1"
                     /locus_tag="POA77_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDI6367821.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2210..2560
                     /locus_tag="POA77_004493"
     CDS             2210..2560
                     /locus_tag="POA77_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HDI6367822.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2686..3246
                     /locus_tag="POA77_004494"
     CDS             2686..3246
                     /locus_tag="POA77_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6367823.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3249..6215
                     /locus_tag="POA77_004495"
     CDS             3249..6215
                     /locus_tag="POA77_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HDI6367824.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6224..6640
                     /locus_tag="POA77_004496"
     CDS             6224..6640
                     /locus_tag="POA77_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367825.1"
                     /translation="MFRFLRRPLAEVANYSNDRWEAPQRASRLAASVKRYKTSEMLRF
                     IFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSADSNPEMVEK
                     MAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
BASE COUNT         1461 a         2104 c         1996 g         1416 t
ORIGIN      
        1 cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg
       61 ccgactacct tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg
      121 cgggaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
      181 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
      241 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
      301 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
      361 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
      421 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
      481 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
      541 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
      601 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
      661 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
      721 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
      781 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
      841 ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
      901 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
      961 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     1021 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     1081 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt
     1141 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     1201 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     1261 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     1321 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     1381 gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag
     1441 ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat cgtgcgggag
     1501 ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc cagcctgcgc
     1561 gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc
     1621 gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg
     1681 ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc
     1741 catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc
     1801 atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc cttgctccgc
     1861 agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt ctctacgacg
     1921 atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc gcttgatgcg
     1981 ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg gattcactac
     2041 ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt cctccctgcg
     2101 tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct ccgctgctat
     2161 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa tggaggtggt
     2221 agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg gttgcgacac
     2281 ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg gcttcgtcaa
     2341 ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct gcgtgtgctg
     2401 caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg tggagcattt
     2461 ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga ccgaaaccag
     2521 cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct aacctgatgt
     2581 cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat tgacatatct
     2641 cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac tggacagcgc
     2701 attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact ggaaggcgtc
     2761 aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg tccgcaactg
     2821 gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag catggatcgc
     2881 ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca acgcggcgtg
     2941 catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc gatggcgaac
     3001 ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat ccgcgagcgt
     3061 cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag gaagaaatcc
     3121 ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga gcaaaagacc
     3181 aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt gagaacggat
     3241 cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc ctgctggcgt
     3301 tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc gacctctcga
     3361 tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag ctctgttacc
     3421 tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc ttgttgaagc
     3481 tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc cagcgggagc
     3541 agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg cccttcacca
     3601 tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa accgacaaag
     3661 gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc attctgcccg
     3721 ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac cggcgcatct
     3781 acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac gatctgctca
     3841 agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct ccggccaagc
     3901 caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag gcactcgatc
     3961 tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt gcccgcgagg
     4021 gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc tacgccactc
     4081 tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc gacctgcacg
     4141 accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag cagttccagg
     4201 cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt caggcgctga
     4261 tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc atgtcctggg
     4321 attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat gacttcgatt
     4381 tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg gaattccttg
     4441 ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc attgaggtgc
     4501 tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg accggcttca
     4561 tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg cgctactacg
     4621 aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc tgggtgcagg
     4681 gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag ttcaccagcc
     4741 tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa tatctgcatg
     4801 agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg gcagccaacg
     4861 acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg gatgcggcgg
     4921 tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg cacgtcaaga
     4981 tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac ttcacgcact
     5041 tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc ctggccgacg
     5101 cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc tacgcgaagc
     5161 tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg ttggctgaac
     5221 tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc accacatcat
     5281 catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg cacatcaacc
     5341 caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac caatacgcgc
     5401 cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg ctcgacggcc
     5461 tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg gcgggcttca
     5521 ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg cgcatccgcg
     5581 acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac gcgctcaagc
     5641 cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac gaaatcctgc
     5701 ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc aggaaactcg
     5761 gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc cgcatcgagc
     5821 gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc gtgcatgccg
     5881 ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc aaccgccttg
     5941 gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg
     6001 tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg catgcgttgc
     6061 gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca ctcggctggg
     6121 agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc ggcgcgggga
     6181 agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc gttttctgag
     6241 acgacccctg gctgaagtgg ctaattacag taacgaccgc tgggaagcac cgcaacgtgc
     6301 ctcacgcctg gctgccagcg taaagcgtta caagacatca gaaatgctgc gctttatttt
     6361 tgctaccatt gcctacgatc ccgatccaga tttaaccccg ctgactgtga gacgtttgtg
     6421 caaggcattg ttcggcagaa ccggaagcca gtggctggtt gttgaagttt ttggggagaa
     6481 ggggcgtcag caccgcagcg cggacagcaa tccggagatg gtggaaaaaa tggctgccag
     6541 atatcgtcat gccgccgaac tacactggtc cgcaacgctg gcagaaattg aacgggtaaa
     6601 gcggctttat cagacgaaaa ttaagaaatc caaaaaataa gtgggttgac tgccattgtt
     6661 gttcttctcc ttcatcatta atgctaatgt atgcatacat tagcattaat gatggtgcgt
     6721 taaagtatca ctggtataat ctggtaaaga acatattgag aatgagaact tatcatgtcg
     6781 tcacgcaact ctgtacatga ttttataatg ctgctcccgg cgctgctgtt ggttgggtgg
     6841 ttagttttgg tgataattct catgtttagg gatgataaat gtgacaaaac ttaccgtgaa
     6901 ggttcgtttc ccgaatttat ttgtctccat tctgatcgct cgtcacttcg agaaaccgat
     6961 tctgagagac tcattgt
//
LOCUS       DAMZII010000204         6967 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZII010000204 DAMZII010000000
VERSION     DAMZII010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.205"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            270..2378
                     /locus_tag="POA77_004497"
     CDS             270..2378
                     /locus_tag="POA77_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI6367826.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            2445..6653
                     /locus_tag="POA77_004498"
     CDS             2445..6653
                     /locus_tag="POA77_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6367827.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGLWWILGWSERVPGAEDVLPAPLPPYRVLTGLADCFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            6665..>6967
                     /locus_tag="POA77_004499"
     CDS             6665..>6967
                     /locus_tag="POA77_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HDI6367828.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDIMNEAKEKDLGTYKKSTLKTEKITRGLFSNDEITLIYFSE"
BASE COUNT         1654 a         1816 c         2163 g         1334 t
ORIGIN      
        1 ctttgcatat caggtttttt attactcgct gtaatgtacg agcctgtcgt gaataacgtc
       61 gagaacccat ccgttgcgtt tgtgattatt ttgttgacta aacagacacc cgtttctctg
      121 aagtaaaatc ccagactaaa tcatcacata accatgacat ttttctgata ttccccggta
      181 acgccagatg tcgactcgct tatctcatcc gcccgacatg ttgtacatgc aatatgtgcg
      241 gattattatt cattttcacg gaggttgcta tgtcaaccgg attacgtttc acacttgaag
      301 tggacggcct gccaccggac gcgtttgcgg tggtttcctt tcatctcacc cagtcactct
      361 cttcactttt ttccctcgac ctttctctgg tcagtcagca gtttctttcc cttgaatttg
      421 cgcaggtgct ggacaaaatg gcctacctga cggtatggca gggcgatgac gtacagcgcc
      481 gggtgaaagg tgtggtgacc tggtttgaac tgggggagaa cgacaaaaac cagatgctgt
      541 acagcatgaa ggtgtgcccg ccgctgtggc gcacagggct gcgccagaac ttccgtatct
      601 tccagaatga ggacatcgaa agcatcctcg ctacgatcct gaaggaaaac ggtgtgaccg
      661 agtggagtcc gctgttcagc gagccgcatc cttcccgtga gttttgtgtc cagtacggcg
      721 aaaccgatta cgatttcctg tgccggatgg cggcggagga aggcatcttc ttttatgagg
      781 agcacgcgca aaaaagtacc gaccagagcc tggtcctgtg cgacaccgtg cgttatctgc
      841 cggagtcctt tgagatcccc tggaatccga acacccgtac cgaggtgagc accctctgca
      901 tcagccagtt ccgctacagt gcacaaatcc gcccttcttc cgtggtgacc aaggactaca
      961 cctttaaacg acccggctgg gcagggcgtt ttgatcagga aggccagtac caggattacc
     1021 agcgcacgca gtatgaagtg tatgactacc ccggacgttt caagggtgcc cacgggcaga
     1081 actttgcccg ctggcagatg gacggctggc gaaacaatgc agaaaccgcg cggggaatga
     1141 gtcgctcgcc ggagatatgg ccgggacgac gaattgtgct gacggggcat ccgcaggcga
     1201 acctgaaccg ggaatggcag gtggtggcaa gtgaactgca cggcgaacag ccacaggcgg
     1261 tgccaggacg gcagggagcg gggacggcgc tggagaacca ttttgcggtg atcccggcag
     1321 acagaacatg gcgaccacag ccgttgctga aaccgctggt cgacggcccg cagagcgctg
     1381 tcgtgacagg accggcaggc gaggaaatct tctgcgacga acatggtcgc gtgcgggtga
     1441 agttcaactg ggaccgttat aacccggcag accaagacag ttcgtgctgg atccgtgtgg
     1501 cacaggcgtg ggcaggcacc ggttttggcc acctggcgat accgcgtgtg ggtcaggagg
     1561 tgattgtgga cttcctcaac ggcgatccgg accagccgat tattatgggg cgtacctacc
     1621 accaggaaaa ccgcacaccc ggcagcctgc cggggacgaa gacgcagatg accattcgtt
     1681 cgaaaaccta taagggcagc gggtttaatg aactgaagtt tgacgatgcg acagggaaag
     1741 aacaggtcta catccacgcg cagaagaaca tgaacaccga ggtgctgaat aaccgcacca
     1801 cggatgtgat aaacaaccat gctgagacca ttggcaacaa tcagatgatt gcggttacca
     1861 acaatcagat acagacggtg ggcgttaacc agatagagac ggtgggcagt aaccagatca
     1921 ttaacgtggg ttctgttcag gtggaaacga ttggacttgt tcgtgcgctg accgtgggcg
     1981 tggcgtacca gacgacggta ggtggcatta tgaacacttc ggtggctctg atgcagtcct
     2041 cgcagatcgg tttgcataaa tcgttgaggg tcgggctggg ttatgacgtc aaagtcggaa
     2101 ataacgttac cttcaccgtt ggtaaaacga aaaaggatga taccgggcag accgcgattt
     2161 actccgccgg tgagcatctg gagctctgct gtggtaaggc aaggctggtg ctgacgaagg
     2221 acggacaaat ttttctcaac ggcacaaaaa ttcatttgca gggtaaggag caggttaatg
     2281 gtgactcact gttgattaac tggaactgtg cagcctcgaa atccccaccg aagacccctg
     2341 atgaaaagca ggatacgccg gatatgagag agtactgatc ccgtacctgt tcggtattca
     2401 gtaacaaaca agcattaaaa ataaacagaa gaaggaattg aatcatgagc ggaaaaccag
     2461 cggcgcgtca gggagatatg actcagtatg gcggtcctat tgtccagggt tcggcaggtg
     2521 taagaattgg cgcgcccacc ggcgtggcgt gctcggtgtg tccgggcggg atgacttcgg
     2581 gcaacccggt aaatccgctg ctgggggcga aggtgctgcc cggcgagacg gaccttgcgc
     2641 tgcccggccc gctgccgttc attctctccc gcacctacag cagctaccgg accaggacac
     2701 ccgcgccggt gggagtgttt ggccccggct ggaaagcgcc ttctgatatc cgcttacagc
     2761 tacgcgatga tgcactggta ctcaacgaca acggcgggcg gagcattcac tttgagccgc
     2821 tgctgccggg ggaggcggtg tacagccgca gcgagtcaat gtggctggtg cgcggtggta
     2881 aggcagcgca gccggacggc cacacgctgg cgcggctgtg gggggcgctg ccgccggata
     2941 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggctg tggtggatac
     3001 tggggtggtc agagcgggtg ccgggtgctg aggacgtact gccagcgccg ctgccgccgt
     3061 accgggtgct taccgggctg gcagactgtt tcgggcggac gctgacgtac cggcgtgagg
     3121 ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga cggtgccggg cgtgagttcc
     3181 gtctggtact gaccacacag gcacagcggg cggaagaggc ccgcacctct tcgctatctt
     3241 cttctgacag ttcccgccct ctctcagcct caccgttccc cgacacactg cccggtaccg
     3301 aatacggccc cgacaggggc atccgtcttt cggcggtgtg gctgatgcac gacccggcat
     3361 acccggaaag cctgcccggt gcgccactgg cgcggtacac gtatacggaa gccggtgaac
     3421 tgctggcggt atatgaccgc agcaatacgc aggtgcgcgc tttcacgtat gacgcgcagc
     3481 acccgggccg gatggtggcg caccgttacg cgggaaggcc ggagatgcgc taccgctacg
     3541 acgataccgg gcgggtggtg gagcagctga acccggcagg cctgagttac cgctaccagt
     3601 atgagcagga ccgcatcacc gtcacggaca gcctgaaccg gcgtgaggtg ctgcatacag
     3661 aaggcggggc cgggctgaag cgggtggtga aaaaagaact ggcggacggc agcgtcacgc
     3721 acagcgggta tgacgcggca ggaaggctca cggcgcagac ggacgcggcg ggacggcgga
     3781 cagagtacgg tctgaatgtg gtgtccggcg atatcacgga catcaccaca ccggacgggc
     3841 gggagacgaa attttactat aacgacggga accagctgac ggcggtggtg tccccggacg
     3901 ggctggagag ccgccgggaa tatgatgaac cgggcaggct ggtatcggag acatcgcgca
     3961 gcggggagac agtacgctac cgctacgatg acgcgcacag tgagttaccg gcgacgacaa
     4021 cggatgcgac gggcagcacc cggcagatga cgtggagccg ctacgggcag ttgctggcgt
     4081 tcactgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
     4141 cggttcaccg cgaggaaggt atcagccttt accgccacta tgacaaccgt ggccggttaa
     4201 cctcggtgaa agacgcacag ggccgtgaaa cgcagtatga gtacaacgcc gcaggcgacc
     4261 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
     4321 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtacgat gctgccggac
     4381 gtgtcatcag tctgactaac gagaacggca gccacagcga cttcagctac gatgcgctgg
     4441 accggttggt acagcagggc ggctttgacg ggcggacgca acgttatcat tatgacctga
     4501 ccggaaaact cacgcagagt gaagatgagg ggcttgtcac cctctggtac tacgatgaat
     4561 cggaccgtat cactcaccgc acggtgaacg gcgaaccggc agagcagtgg cagtatgatg
     4621 accacggctg gctgacagac atcagccacc tgagcgaagg ccaccgtgtc gctgtccact
     4681 acggctacga cgataagggc cgcctgaccg gcgaacggca gacggtggag aacccggaga
     4741 cgggggaact gctgtggcag catgagacaa cacacgccta caacgaacag gggctggcaa
     4801 accgcgtcac gccggacagc ctgccgccgg tggagtggct gacctacggc agcggttatc
     4861 tggcgggcat gaagcttggc gacacgccgc tgctggagta cacgcgcgac cgcatgcacc
     4921 gtgagacggt gcgcagcttt ggcagcatgg caggcagtaa tgccgcatat aaactgacca
     4981 gcacatacac ccccgcaggc cagttacaga gccagcacct gaacagcctg gtgtatgacc
     5041 gtgactacgg gtggaatgac aacggcgacc tggtgcgcat cagcggcccg cgacagacgc
     5101 gggaatacgg ctacagcgcc acgggcaggc tggagagcgt gcgtaccctc gcaccggacc
     5161 tggacatccg catcccgtat gccaccgacc cggcgggaaa ccgcctgccg gacccggagc
     5221 tgcacccgga cagtaccctc acggcgtggc cggataaccg catcgcagag gatgcgcact
     5281 atgtttacca ctatgatgaa tacggcagac tgacggagaa gacggacctt atcccggcgg
     5341 gtgttatccg gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgtc
     5401 tggtgttcta cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg
     5461 acccgctggg acggcgaatg gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt
     5521 ggatgtcgct gtcgcgtaaa ccggagatga cgtggtacgg gtgggacggc gaccggctga
     5581 cgacggtaca gaccgacacc acgcgtatcc agacggtata tcagccgggg agcttcgcgc
     5641 cgctcatccg catcgaaaca gacaacggcg agcgggagaa agcgcagcgc cgcagcctgg
     5701 cggagaagct gcagcaggaa gggagcgagg acgggcacgg cgtggtattt ccggctgaac
     5761 tggtgcggct gctggacagg ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa
     5821 gccgggcgtg gcttgcgcag tgcgggctga cggtggagca actggccaga caggtggagc
     5881 cggaatacac accggcgcga aaagttcatt tttaccactg cgaccaccgg ggcctgccgc
     5941 tggcgctcat cagcgaagac ggcaatacgg cgtggcgcgg ggagtatgat gaatggggca
     6001 accagcttaa tgaagagaac ccgtattacc tgcaccagcc ataccgtctg ccggggcagc
     6061 agcatgatga ggaatcaggg ctgtactata accggaaccg gtactatgac ccgctacagg
     6121 ggaggtatat tacacaagac cccattgggc tggcgggggg atggaacctg tataattacc
     6181 cactgaatcc gataataagg atggatcctt tgggtttgta taatttatat caattattat
     6241 atgatgtttg gcatgatgat tcatatggaa catcatcaat tgatattact ggcagtggag
     6301 atctaatatc attaggtggt catgcaggac ttggcgttgc gtttgctaaa aagaaaggtg
     6361 aaatgttatc tgatatttgt atttatgcta cagcatgcgg acatgcagga attggtggtg
     6421 ggataaatgc ggctatcaca tattctgaga ccaagtcttt acctacatcg ggagtcagca
     6481 attcagtagg tgtaacggtt ggcggcggag ttggggggca ttttgcgtat acttatgtag
     6541 tggatgttga taatccagaa tcatcgacag aatctgttgg tatcggtgca ggtgttgacg
     6601 cttcagttat gactctggct tgtagaacgt ggcaagaatg ctgggtcaat taacaggagg
     6661 aaatatgaaa aaatatatat ttttagtttt atttttattg tgtggttgtt ctccagtgga
     6721 catagagcca gctagacaag aagttaatat ctttcgtggt ttatatcaga cagggaaata
     6781 cagtgatata tataaaatca cttcaagtga ttttcagact gccacatctg aaaaaaaatt
     6841 tatcgacata atgaacgaag caaaagaaaa agatcttggg acttataaaa aatcaaccct
     6901 taaaactgaa aaaatcactc gcggattatt ttcaaacgat gaaattacat tgatatattt
     6961 ctcagaa
//
LOCUS       DAMZII010000205         6962 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZII010000205 DAMZII010000000
VERSION     DAMZII010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.206"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1281)
                     /locus_tag="POA77_004500"
     CDS             complement(<1..1281)
                     /locus_tag="POA77_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6367829.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFV"
     gene            complement(1396..1707)
                     /locus_tag="POA77_004501"
     CDS             complement(1396..1707)
                     /locus_tag="POA77_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6367830.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            1906..2604
                     /locus_tag="POA77_004502"
     CDS             1906..2604
                     /locus_tag="POA77_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6367831.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(2649..3548)
                     /gene="eamA"
                     /locus_tag="POA77_004503"
     CDS             complement(2649..3548)
                     /gene="eamA"
                     /locus_tag="POA77_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDI6367832.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            3743..4930
                     /gene="ydeE"
                     /locus_tag="POA77_004504"
     CDS             3743..4930
                     /gene="ydeE"
                     /locus_tag="POA77_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDI6367833.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTISVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            5057..5152
                     /gene="mgtS"
                     /locus_tag="POA77_004505"
     CDS             5057..5152
                     /gene="mgtS"
                     /locus_tag="POA77_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDI6367834.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(5371..6261)
                     /gene="dgcZ"
                     /locus_tag="POA77_004506"
     CDS             complement(5371..6261)
                     /gene="dgcZ"
                     /locus_tag="POA77_004506"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDI6367835.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(6516..6908)
                     /gene="ydeI"
                     /locus_tag="POA77_004507"
     CDS             complement(6516..6908)
                     /gene="ydeI"
                     /locus_tag="POA77_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDI6367836.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1986 a         1627 c         1452 g         1897 t
ORIGIN      
        1 aacaaaaggt aaatagatca ttgtcgccac acctaaacat aaaaaaccaa ccagtaaagc
       61 cagccagttc cccccggttc cgaggaaagc aattaaaggt ccgggcgtag tccagggcac
      121 ggcataagca accggtggaa taatttccat cgaaacaaag aagtaaccaa tggcacagtt
      181 aacaatggga accataataa acggcaccat catgatgggg ttaagtacaa taggaaggcc
      241 aaacattatc ggttcattga tattgaaaat accggggata aatgacattt ttgccagatc
      301 acggtattcc gcacgcttag aagcgataaa aatagccaac aataacccca acgtcatacc
      361 tgaaccgccg cagttggcga atgcatcatt aatagaagtc caggtaatcg gatatggcgc
      421 gccccaggta gtgccatgtt gagcggccca ggagagattc tccagattag cctcagaaaa
      481 aacggtttct cgaattgccg atgtggtatt aggtccgtgg atccccagca cccacaggaa
      541 attacccaca gcccccagga taattaccgc aaagatattc gttcccatat gtttgagcgg
      601 cgtctggatt aatgtgtaaa taagatcgtt tagtcctgcc ggggatatca gtgtcaggca
      661 ataattaagt gcggaaaaga acaccatgac aaaaaatatt ggcaataaaa ctttaaatga
      721 acgcgctacc gcaggcggta cagctgccgg catcgttatg gtgatttttg ggtttcgagc
      781 taagcgacaa aatatttcac tggtgatcaa tgcaacgata acagcaacaa acaacccctg
      841 cgcgccgaga tatttggtcg tcaggaatgc ttgcccatct atcatctgat atggcgtata
      901 aacaataaaa aatgctccta ttgccgttaa tccgcataaa agatcatctt gctcatagct
      961 aatcgccata ttcctggcga ccaggaaagc aatcataatt gacatgatat ttacagaacc
     1021 attcattacc ggagtaaaaa tagcttgtgc tttttcaagg ttggggaaaa tgctattcag
     1081 atgcagtaat ccggcaataa aaccgtctgg cgataatatg gcaaagttaa ttaatataat
     1141 taatgaactt gccatgataa ttggaaacgt aaaaacgaat ccatctctca ccgctgcaac
     1201 atgtttttgt gagtttaact tgatagcaac tggaacaaga aaacgttcca ttccacgttc
     1261 gaatgaggcc attaatccca tttttttata cctgttgttt tggatattta tacatcccaa
     1321 taaagcatgt atgccatatc cattttatga tgtgtgaaaa gttagttcag gtaatagttt
     1381 aataatatta gattcttact cttgcagtag tttcatcgca aattcaagaa ctgcctgtcc
     1441 gttcatggtg ccgtagtctt tcatctcgat aacggctaca ggcattttcc cctgtgcaac
     1501 ggcttcaatc tctggtttct gaaagcgaac ttgcgggcca agtaaaacaa cgtcaacttc
     1561 gttattttta attttccctt tagcctctgc aatcgccaat gcggaaatat taacttcaag
     1621 tgaaatagcg ttagcatgat caatcatacg tttaaccagc atactggttg acatgcccgc
     1681 agcgcaaact aacaatattt ttttcatatt ttcccttact ggtatataac agattacatt
     1741 gtttaaatat catatcccgc agtagctata cgtcctggta cattgatcac agtaatacag
     1801 tgataaagtt gttcaacatc agggtgatta ttaaacgact cggccatacg ataaaccaat
     1861 caggactcaa ctccttgttt tcaatggctc ttaaaaggtg caaaagtgat ttttcaaaag
     1921 attgcccgct tacttaaatc cgaaatcaat ggcaattcat ggcatgttgg tgatttgttg
     1981 ccgtcagaag cggaactcgc tgttcgttat aatgtttcgc gtaataccct gcgtaaggca
     2041 ttatccttgc tggaaggcga aggaattatt cacagaaagc atggttcagg aacatacatt
     2101 caaaaaaaga attttgttgc acacattgat cacatgaaca gtttcagtga aattgcgcat
     2161 aaaagcggca aagaggcagg gagtcagatt atgaaatttg aagtgcagga tgcttcaccc
     2221 actattgcga ctgagctgaa tttagtgact ggtgagcagg tttattacat aaagagactg
     2281 cgattcattg aggataatgc agcacaatta gaagaaacgt ggatgtcagt agcacgtttt
     2341 cctgatttaa ccgtgtcaca tatgcaaaaa tccaaatttt catatattga gaatgaatgc
     2401 gggatcaaaa tcattggcac ctttgaaact ttctccccta cttttcctac cccagaaatc
     2461 gccagtattt tacggatcag cccacgggat cccatactta aaattcagac ccaggctgtg
     2521 gacagtaact ctattccgct ggattattcg ttactttaca gcaatatttt cgagttccag
     2581 gtaaagtact ttttcccgcg ataaaagcag acgcacagag tgctgacatc acattctgtg
     2641 cgtatggttt agcctctaac ctttaccgct ttacgccacc gcaagccaaa tacattgata
     2701 tacagcccgg tcataatgag caccgcacct aaaaattgca gacccgttaa gcgttcatcc
     2761 aacaatagtg ccgcacttgc cagtccgact acgggcacca gtaacgataa cggtgcaacc
     2821 cgccaggttt catagcgtcc cagtaacgtc ccccagatcc cataaccaac aattgtcgcc
     2881 acaaacgcca gatacatcag cgacaagatg gtggtcatat caatagtaac cagactgtga
     2941 atcatggatg cggaaccatc gagaatcagc gaggcaacaa agaagggaat gattgggatt
     3001 aaagcgctcc agattaccag cgacatcacc gccggacgcg ttgagtgcga catgatcttt
     3061 ttattgaaga tgttgccaca cgcccaacta aatgctgcag ccagggtcaa cataaagccg
     3121 agcatcgcca catgctgacc gttcagacta tcttcgatta acaccagtac gccaaaaatc
     3181 gctaaggcga tccccgccaa ttgtttgcca tgcaatcgct ccccgaaagt aaacgcgcca
     3241 agcacgatag taaaaaacgc ctgtgcctgt aacaccagcg aagccagtcc agcaggcata
     3301 ccgaagttaa tggcacaaaa aagaaaagca aactgcgcaa aactgatggt taatccatac
     3361 cccagcagca aattcagtgg tactttcggt cgtgcgacaa aaaagatagc cggaaaagcg
     3421 accagcataa agcgcaaacc ggccagcatc agcggtggca tgttatgaag ccccactttg
     3481 atgaccacaa aatttagccc ccatacgacc actaccagta gcgccaacac cccatctttt
     3541 cgcgacattc taccgcctct gaatttcatc ttttgtaagc aatcaactta gctgaattta
     3601 cttttcttta acagttgatt cgttagtcgc cggttacgac ggcattaatg cgcaaataag
     3661 tcgctatact tcggattttt gccatgctat ttctttacat ctctaaaaca aaacataacg
     3721 aaacgcactg ccggacagac aaatgaactt atccctacga cgctctacca gcgcccttct
     3781 tgcctcgtcg ttgttattaa ccatcggacg cggcgctacg ctgccattta tgaccattta
     3841 cttgagtcgc cagtacagcc tgagtgtcga tctaatcggt tatgcgatga caattgcgct
     3901 cactattagc gtcgttttta gcctcggttt tggtatcctg gcggataagt tcgacaagaa
     3961 acgctatatg ttactggcaa ttaccgcctt cgccagcggt tttattgcca ttcctttagt
     4021 gaataacgta aagctggtgg tgctcttttt tgccctcatt aactgcgcct attctgtttt
     4081 tgctaccgtg ctgaaagcct ggtttgccga caatctttcg tccaccagca aaacgaaaat
     4141 cttctcaatc aactacacca tgctaaacat tggctggacc atcggtccgc cgctcggcac
     4201 gctgttggtg atgcagagca tcaatctgcc cttctggctg gcagctatct gttccgcgtt
     4261 tcccatgctt ttcattcaaa tttgggtaaa gcgcagcgag aaaatcatcg ccacggaaac
     4321 aggcagtgtc tggtcgccga aagttttatt acaagataaa gcactgttgt ggtttacctg
     4381 ctctggtttt ctggcttctt ttgtaagcgg cgcatttgct tcatgcattt cacaatatgt
     4441 gatggtgatt gctgatgggg attttgccga aaaggtggtc gcggttgttc ttccggtgaa
     4501 tgctgccatg gtggttacgt tgcaatattc cgtgggccgc cgacttaacc cggctaacat
     4561 ccgcgcgctg atgacagcag gcaccctatg tttcgtcatc ggtctggtcg gttttatttt
     4621 ttccggcaac agcctgctat tgtggggtat gtcagctgcg gtatttactg tcggtgaaat
     4681 catttatgcg ccgggcgagt atatgttgat tgaccatatt gcgccgccag gaatgaaagc
     4741 cagctatttt tccgcccagt ctttaggctg gcttggtgcc gcgattaacc cattagtgag
     4801 tggcgtagtg ctaaccagcc tgccgccttc ctcgctgttt gtcatcttag cgttggtgat
     4861 cattgctgcg tgggtgctga tgttaaaagg gattcgtgca agaccgtggg ggcagcccgc
     4921 gctttgttga tttaagtcga acacaataaa aatttaattc agccttcgtt taggttacct
     4981 ctgctaatat ctttctcatt gagatgaaaa ttaaggtaag cgaggaaaca caccacacca
     5041 taaacggagg caaataatgc tgggtaatat gaatgttttt atggccgtac tgggaataat
     5101 tttattttct ggttttctgg ccgcgtattt cagccacaaa tgggatgact aatgaacgga
     5161 gataatccct cacctaaccg gccccttgtt acagttgtgt acaaggggcc tgatttttat
     5221 gacggcgaaa aaaaaccgcc agtaaaccgg cggtgaatgc ttgcatggat agatttgtgt
     5281 tttgctttta cgctaacagg cattttcctg cactgataac ggatcgttga cacagtagca
     5341 tcagttttct caatgaatgt taaacggagc ttaaactcgg ttaatcacat tttgttcgtc
     5401 aataaacatg cagcgatttc ttccggtttg cttaccctca tacattgccc ggtctgctct
     5461 tccaatgacc acatccagag gctcttcagg aaatgcgcga ctcacacctg ctgtcacggt
     5521 aatgttgata tgcccttcag aatgtgtgat ggcatggtta tcgactaact ggcaaattct
     5581 gacacctgca cgacatgctt cttcatcatt agccgctttg acaataatga taaattcttc
     5641 gcccccgtag cgataaaccg tttcgtaatc acgcgtccaa ctggctaagt aagttgccag
     5701 ggtgcgtaat actacatcgc cgattaaatg cccgtaggta tcattaacca atttaaatcg
     5761 gtcaatatcc aacaacatta aataaagatt cagaggctca gtgttgcgta actgatgatc
     5821 aaaggattca tcaagaaccc gacgccccgg caatcccgtc aaaacatcca tattgctacg
     5881 gatcgtcagc aaataaattt tgtaatcggt taatgccgca gtaaaagaaa gcaacccctc
     5941 ctgaaaggcg tcgaaatgcg cgtcctgcca gtgattttca acaatagcca gcattaattc
     6001 ccgaccacag ttatgcatat gttgatgggc agaatccatt agccgaacgt aaggtaattc
     6061 atcgttatcg agtggcccca gatgatcaat ccaccgacca aactggcaca gtccataaga
     6121 atggttatcc gttatttctg gcttactggc atctctcgcg accacgctgt gaaacatact
     6181 caccagccac tggtagtggg catcgatagc cttattgaga tttaacaaga tggcatcaat
     6241 ttccgttgtt ttcttgatca ttgccactcc tttttcacag ttccttgtgc gcgctattct
     6301 aacgagagaa aagcaaaatt acgtcaatat tttcatagaa atccgaagtt atgagtcatc
     6361 tctgagataa cattgtgatt taaaacaaaa tcagcggata aaaaagtgtt taattctgta
     6421 aattacctct gcattatcgt aaataaaagg atgacaaata gcataaccca ataccctaat
     6481 ggcccagtag ttcaggccat caggctaatt tatttttatt tctgcaaatg agtgacccga
     6541 acgacggccg gcgcgctttt cttatccaga ctgccgctaa tgttgatcat ctggtccggc
     6601 tgaacttctc gtccatcaaa gacggccgca ggaataacga cattaatttc accgctctta
     6661 tcgcgaaaaa cgtaacggtc ctctcctttg tgagaaatca aattaccgcg tagtgaaacc
     6721 gaagcgccat cgtgcatggt atttgcgaaa tcaacggtca ttttttttgc atcatcggtt
     6781 ccgcgatagc catcttctat tgcatgaggc ggcggtggcg ctgcatcctg ttttaaaccg
     6841 ccctggtcat ctgccaacgc ataaggcatg acaagaaaac ttgctaatac aatggcctga
     6901 aatttcatac tgactcctta attgcgtttg gtttgactta ttaagtctgg ttgctatttt
     6961 ta
//
LOCUS       DAMZII010000206         6904 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZII010000206 DAMZII010000000
VERSION     DAMZII010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.207"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..438
                     /locus_tag="POA77_004508"
     CDS             <1..438
                     /locus_tag="POA77_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6367837.1"
                     /translation="QEILESNDNSQDDIIEAWQKAFSSPLCQFDKYYNYVKGKTNIDT
                     HQGVKGLEFPRVLVLIDDSEAKGFMFSYDKLFGVKEKTKTDLDNEKEGKDTSEDRTRR
                     LFYVTCSRAQKSLAILAYTDNTEMLRKKLLHKGWFDSSEIEII"
     gene            602..934
                     /locus_tag="POA77_004509"
     CDS             602..934
                     /locus_tag="POA77_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HDI6367838.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQKISVPLHQRHGIDVVSFGNS
                     LHDPDCYYLIRAFDSAESMAMVLNAFYASDDWRSGPREDIVGSIETSIKTVMNLPSEN
                     VEGLRVQS"
     gene            complement(1058..2998)
                     /locus_tag="POA77_004510"
     CDS             complement(1058..2998)
                     /locus_tag="POA77_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6367839.1"
                     /translation="MAQRDNLYRRSSGIYVVRIAVPARYRLYTGQREIHTSTETNDIR
                     KARAIAAGLLVVWQRCLGEYQELDREKLVSSAPALAGEGMISLAEFCGLTSASLQQVA
                     QRLIDTNIPLFWLADGRPAYYVEDLSLVDKDDPEASGFIIDSAINIGIRGSLTGYVQP
                     LVLSRILRQMIAGELLDAETAFKSSEASSKAAWFVDFPGVEISPSSLMIHRVQAERLR
                     LFWLSHSAAVSPIMPEKPKAIPPVLLPSSIPDNEYVNRKYYSVKMSELCEKYIRYKRG
                     GKFTEKAENRVREGFGLLLEVMGDSTLEKVDRDFLRKYESLLRSIPARRDLAKIRYKI
                     NDVHELIVKAKENSDPLMSDNAVRKYMRILFEAFQWADGEGIFIKSPANKFFAPIENE
                     KMDQDFKSNFTDEDLKAIFGMPWYKRGTVDKNSQGQFHHYRAFHYWLPLIGFFTGARI
                     NELCQLYLDDIKQDAVGFYFFEISNERADQSLKTIHSRRKIPLHPTLIKLGLVRYCEA
                     LKKAGHERLFPELPYHPVKGYGDKASDWFNRSLLKERLGFEKESKKSFHSFRHTFSTR
                     LKQAGIDSETRAQFVGHIRGSGETENRYSKDHPPAALYAVLESVTLGLPEVAPFVCED
                     GVVAVADALRIKQSNKSRKEPQ"
     gene            complement(3086..3161)
                     /locus_tag="POA77_004511"
     tRNA            complement(3086..3161)
                     /locus_tag="POA77_004511"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3126..3128),aa:Thr,seq:cgt)
     gene            complement(3276..4529)
                     /gene="proA"
                     /locus_tag="POA77_004512"
     CDS             complement(3276..4529)
                     /gene="proA"
                     /locus_tag="POA77_004512"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDI6367840.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(4541..5644)
                     /gene="proB"
                     /locus_tag="POA77_004513"
     CDS             complement(4541..5644)
                     /gene="proB"
                     /locus_tag="POA77_004513"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDI6367841.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            5932..>6904
                     /gene="phoE"
                     /locus_tag="POA77_004514"
     CDS             5932..>6904
                     /gene="phoE"
                     /locus_tag="POA77_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDI6367842.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDY"
BASE COUNT         1880 a         1668 c         1584 g         1772 t
ORIGIN      
        1 caagaaatcc tagaaagtaa cgataactca caagatgata tcattgaagc ttggcagaaa
       61 gcgtttagct ctcctttatg tcaatttgat aaatattata attatgtcaa agggaagaca
      121 aacatcgata ctcatcaggg agttaaaggt ctagaatttc ctcgtgttct agttttaatt
      181 gatgattctg aggctaaagg ttttatgttt agttatgaca aacttttcgg tgttaaagaa
      241 aaaaccaaaa ctgatttaga taatgaaaaa gaaggtaaag atactagtga agacagaact
      301 agaagactat tttacgtcac atgtagtcga gcacaaaaaa gcctcgccat tcttgcttat
      361 acagataata cagaaatgct aagaaaaaaa ctactacata aaggatggtt tgattcgagt
      421 gaaatagaaa taatttaatt aaaagcagta ataggatatt ttaaagtgaa ctgacatcat
      481 aaaaatgttg gttcacttaa tacgaaccat cggtaagaaa caaaagttgg tgttgaataa
      541 tatgttcact tatacatctt gttattttaa gcacacagtc gaccattaat ggagaataaa
      601 attgagaacc gtcgaaatac tgcaatacac cttacgaaaa ggcagcggcg ccgcttttca
      661 cgccataatg cagaaaatca gtgttcccct gcaccaacgc cacgggatcg atgttgtttc
      721 gtttggtaac tcgttacatg acccggattg ctactatctg attcgcgcct ttgacagtgc
      781 tgaaagcatg gctatggttc ttaatgcttt ttacgcaagc gatgactggc gcagcggccc
      841 acgcgaagac atcgttggca gcatagagac cagcattaaa acagtgatga atctaccatc
      901 agaaaacgtg gaagggctgc gggtgcaatc gtaggtcatc aaatttcacc aaacgcgttt
      961 tgagcgtatc caaattcagc actatgaaaa tcaccaccct taataaaaaa cgcgccagaa
     1021 tcgttctgag cgcgttagag caaaagagaa attcaggcta ttgcggttct tttcgcgatt
     1081 tgttgctctg tttaatccgt agcgcatcag ctacagcaac cacaccatct tcgcaaacaa
     1141 atggagcaac ttccggtagc cctaacgtaa cgctctcaag cacagcatag agggctgctg
     1201 gtggatgatc tttgctatac cggttctctg tctcgccaga accgcggata tgcccaacaa
     1261 actgcgcacg cgtctcgctg tcgattccag cttgtttaag tctagtagaa aacgtgtgac
     1321 ggaatgaatg gaaagatttt ttgctctctt tttcaaagcc taaccgttct ttgagcaacg
     1381 agcgattaaa ccagtccgaa gctttatcgc catatccttt tacagggtgg taaggaagct
     1441 cgggaaataa acgttcatgg cctgctttct tcaatgcttc gcaatagcgg actaatccaa
     1501 gtttgatcaa tgtcggatgc aacggtattt tccgccgcga atgaatagtt tttagcgact
     1561 gatcggcacg ctcattgctg atttcgaaga aataaaaccc gaccgcatcc tgcttaatat
     1621 cgtcaagata aagctgacat aattcattta tcctcgcgcc ggtaaaaaag ccaattagcg
     1681 gtagccagta atgaaatgca cgataatgat ggaattgccc ctgactattt ttatcgacag
     1741 tcccacgttt ataccaaggc ataccaaaaa tagctttcaa atcctcatca gtgaaattag
     1801 atttgaaatc ctgatccatc ttttcatttt caataggtgc aaagaattta ttcgcaggag
     1861 acttaataaa aatgccttct ccatcagccc actggaaagc ctcgaagaga attcgcatat
     1921 atttacgaac ggcattatcg ctcattagag gatcactatt ctcttttgcc ttgacaatca
     1981 attcatgaac atcattgatt ttatatctta tttttgccag atcgcgcctt gcaggtatcg
     2041 accttagtaa gctctcatat ttacgaagga agtcacggtc aactttttca agcgttgaat
     2101 cacccatgac ttcaaggagt aagccaaaac cttcccgaac tcgattttct gccttttccg
     2161 tgaatttccc tccacgcttg tagcgaatat atttttcaca taactcgctc attttcactg
     2221 agtagtattt acgatttaca tattcattat ccggtattga tgagggcaat aatacagggg
     2281 gaattgcttt tggtttttca ggcatgattg gagaaaccgc tgcgctatgt gacagccaaa
     2341 ataatcgtaa gcgctcagct tgcacacgat gaatcattaa tgaactaggc gagatttcga
     2401 caccgggaaa gtcaacaaac caagcggctt tggatgaagc ctctgaggat ttaaatgcgg
     2461 tttctgcatc cagcagctcg ccagcgatca tctgacgtaa tatacgtgat aaaaccaggg
     2521 gctgaacata accggtcaga gaacctctga tcccgatgtt aatggcacta tcgatgataa
     2581 atccagaagc ctcaggatca tccttatcaa cgagtgaaag atcttctaca tagtaggcag
     2641 gacgcccatc agcaagccaa aaaagcggga tatttgtatc aattagtcgc tgcgccactt
     2701 gttgcaggga agcgctcgtt aaaccgcaaa attcagcgag gctgatcatt ccttcgccag
     2761 ccaaagcagg cgcactgctt accaactttt ctctatccaa ttcttgatac tcacccagac
     2821 atcgctgcca aacaaccaat agacctgcgg caatcgctcg cgcttttctg atgtcgtttg
     2881 tttctgttga agtgtgaatc tcacgctgtc ccgtgtagag acggtatcgg gcgggaactg
     2941 caatacggac aacataaata ccgctgctac ggcgatacag attatcacgt tgagccatcg
     3001 gctgtatgac ctctatgtag gagaggctac gcgatttcac tctgaatgaa tttaatgtta
     3061 attaaatcaa taaaatagaa gtgactggtg ccgataatag gagtcgaacc tacgaccttc
     3121 gcattacgaa tgcgctgctc taccaactga gctatatcgg ccctgaaagg acatgttcac
     3181 gaacgtgaat cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa tggccttgtg
     3241 aatcaaatgg ctacttttgc atcacccggt tttatttacg cacgaatggt gtaatcacca
     3301 atgccgatcc acttgtaagt ggtcagtgct tccagcccca ttgggccacg cgcgtggagt
     3361 ttttgtgtgc ttaccgccac ttccgcaccc agaccaaact ggccgccgtc ggtaaaacgc
     3421 gtagaggcgt taacgtaaac agcggacgaa tccacttcgt taacaaaacg ctgggcgttg
     3481 cgcatatcgc gggtcaggat cgcatcggag tgttgtgtgc cgtgttcacg aatatgggcg
     3541 atggcatcgt caagatcgct gacgattttg acgttcaaat ctaatgacag aaactcatcg
     3601 tcatactctt cggctttaac agcaaccacc ttcgcagggc ctgcctgcaa ctgcgccagt
     3661 gcagctgcat ctgcgtgtaa tgtcacgccg ctttccgcca tttgtttgct taatgcgggc
     3721 aggaagctat cggcgatgtt tttattcacc agcaacgttt caaccgtatt acatgtgctc
     3781 ggacgctgag ttttcgcgtt gacgatcact tttaatgctt cagcgatctc tacactttca
     3841 tcaacgtaaa tatggcatac gcctatacca cctgtgatca ccgggattgt cgactgttca
     3901 cggcacagtt tatgcaaacc agcgccacca cgcgggatca gcatgtcgat gtatttatcc
     3961 atacgcagca tttcactgac cagcgcacgg tcaggattat caatcgcctg cacggcaccc
     4021 gccggtaagc cgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc agcgttagtg
     4081 cgacacgttt ctttgccacc gcgcaggatc accgcattac cggttttcag gcacagcgaa
     4141 gcgacatcaa ccgtcacgtt cgggcgcgct tcataaatca cgccaataac ccccagcggt
     4201 acgcgacgac gctcaagacg caggccgctg tccagtacgc tgccatcgat tacctgcccc
     4261 accggatcgg cgaggttaca cacctggcgc acatcatcgg caatgccttt cagccgtgcg
     4321 ggcgtcagtg ccagacggtc aagcatcgct tcgccaaggc cattggcacg cgcgtcagca
     4381 acatcctggg cgttagcgtt gaggatgatt tcgctttgtg cttccagttc atcggcgatt
     4441 ttttccagca cgcgattttt ttcgcggctg gagagttgcg ctaatttata cgaggcttgc
     4501 ttcgcggcaa tgcccatttg ttccagcatc agcctgctcc ttaacgggta atcatgtcat
     4561 cacggtgaac ggcaaccggg ccgtattcat atcccagtat tgcatcaatt tcttgcgagt
     4621 ggtgcccggc aatacggcgt aatgcatcgc tgttgtaacg actgacgccg tgggcgatat
     4681 cgcgaccttc gaggttgcaa atgcggatga cttcaccacg cgagaaattg ccagtcacgc
     4741 ttttaatgcc tttcggcaac agggagctgc cgcgttcaag aatggcggca gttgcccctt
     4801 catctaccgt gatttcaccc gccggcggcg caccgaaaat ccagcgttta cggttttcaa
     4861 gcggagtcgc ctgggcatgg aacagcgtac cgacggaaat gccttccatc acatcaccaa
     4921 taacgcccgg cttgctgccc gcggcaataa tggtgtcgat acccgcacgg caagccacgt
     4981 cagcggcctg caatttggta ctcatgccgc cagttccgag gcctgaaacg ctgtcaccgg
     5041 caatcgcgcg cagtgcgtca tcaatgccgt aaacatcttt aatcagttct gcctgcggat
     5101 tgctgcgcgg atcagcggta tacaaacctt tttgatcggt cagcagcaac agtttatcgg
     5161 cacccgccag aatcgccgcc agcgcagaaa ggttatcgtt atcgccgacc ttaatctctg
     5221 ccgtagcgac agcatcgttc tcattgatta ccggaacgat attgttatcg agcaacgcac
     5281 gcagggtgtc gcgggcgttc aggaagcgtt cacggtcttc catatcagca cgggtcagca
     5341 gcatctgccc gacgtgaatg ccataaatcg aaaacagctg ttcccacagt tgaatcagtc
     5401 gactctgccc taccgccgcc agcagttgtt tcgaggcgat agtcgctggc agttccgggt
     5461 accccaggtg ctcacgtccg gcggcgatcg cgcccgacgt cacaataaca atccgatgcc
     5521 cggcggcatg taactgcgcg cactggcgaa caagttcaac gatatgggca cggttcagac
     5581 ggcgcgatcc gcctgttagc acactggtgc cgagttttac caccagcgtc tggctgtcac
     5641 tcatgattct ctgccattca attttaggaa aaatgatatc aaacgaacgt tttagcagga
     5701 ctgtcgtcgg ttgccaacca tctgcaagca aagcatggcg ttttgttgcg cgggatcagc
     5761 aagcctagcg gcagttgttt acgcttttat tacagattta ataaattacc acattttaag
     5821 aatattatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
     5881 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
     5941 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
     6001 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
     6061 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
     6121 gaaacacaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
     6181 aataaagcgg agagtgatac cgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
     6241 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
     6301 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
     6361 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
     6421 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
     6481 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
     6541 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
     6601 acaggcaagc gtgcagaagc atgggctaca ggtctgaaat acgatgccaa taatatttat
     6661 ctggcaactt tttattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
     6721 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
     6781 ctgggttatg tcttatcgaa agggaaagat attgaaggta tcggtgatga agatctggtc
     6841 aattatatcg atgtcggggc tacatattat ttcaacaaaa atatgtcagc gtttgttgat
     6901 tata
//
LOCUS       DAMZII010000207         6545 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZII010000207 DAMZII010000000
VERSION     DAMZII010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.208"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..218)
                     /locus_tag="POA77_004515"
     CDS             complement(<1..218)
                     /locus_tag="POA77_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDI6367843.1"
                     /translation="MDFNSGGKKAIERLVEAYGFGTRQALCDHLGVSKSTMATRYMRD
                     IFPADWVIQCALETGTSLNWLTTGHGSK"
     gene            364..567
                     /locus_tag="POA77_004516"
     CDS             364..567
                     /locus_tag="POA77_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDI6367844.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRLPRHRL
                     RKDKKREKVMINLAALTVDALSA"
     gene            632..1141
                     /locus_tag="POA77_004517"
     CDS             632..1141
                     /locus_tag="POA77_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HDI6367845.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAGRAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            1149..1382
                     /locus_tag="POA77_004518"
     CDS             1149..1382
                     /locus_tag="POA77_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDI6367846.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIYLPCGKKWHPSFELQPRQ
                     QAYRGRNKKRFLQRFMVNVANNLRTKRKVFLGS"
     gene            complement(1330..1788)
                     /locus_tag="POA77_004519"
     CDS             complement(1330..1788)
                     /locus_tag="POA77_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367847.1"
                     /translation="MTKQSSEYFQLHYCYYLELMTATFHGRADKLMTAIQLISGTAVF
                     ANTGLEWLFAFPVVVIATIQLVWQPAIIAERASVQSRQYGELLYAEDELSPQFIAQKL
                     KALHHSDSTTFGSLLNPAYKRAAIACGRVDDVNLSFQEKLFAWFAGCLPR"
     gene            1982..2248
                     /locus_tag="POA77_004520"
     CDS             1982..2248
                     /locus_tag="POA77_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDI6367848.1"
                     /translation="MLVKQSPAMHYGHGWIMGEDGKRWHPCHSQDELLSELTTRKRRK
                     SKCMQRKVKWFISFVTEGRVIQYLKMICSVVIRRCRAMAVTFSR"
     gene            2366..2707
                     /locus_tag="POA77_004521"
     CDS             2366..2707
                     /locus_tag="POA77_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HDI6367849.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            2775..3008
                     /locus_tag="POA77_004522"
     CDS             2775..3008
                     /locus_tag="POA77_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDI6367850.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            3008..3235
                     /locus_tag="POA77_004523"
     CDS             3008..3235
                     /locus_tag="POA77_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI6367851.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCVECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            3232..4089
                     /locus_tag="POA77_004524"
     CDS             3232..4089
                     /locus_tag="POA77_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDI6367852.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFKEANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGACCWVGFDPSRGVD
                     SSAVYGVRA"
     gene            4086..6500
                     /locus_tag="POA77_004525"
     CDS             4086..6500
                     /locus_tag="POA77_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDI6367853.1"
                     /translation="MSHADMNNCSGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMVILADVSVQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLYQQGKDKSADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRQQLVYELESRGFNGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     KWRNLY"
BASE COUNT         1733 a         1531 c         1678 g         1603 t
ORIGIN      
        1 tgctttgaac catgccctgt tgttagccaa ttaagcgagg tacccgtttc aagggcgcac
       61 tggattaccc aatctgccgg aaaaatatca cgcatataac gcgttgccat ggtgctctta
      121 gaaacaccta aatgatcaca gagagcctga cgggtaccga acccgtatgc ttcaaccaaa
      181 cgttctatgg ctttcttacc gccgctattg aaatccacaa atcctccaaa gaaatccaaa
      241 attcgttgac agattccaaa agcgatctta aagttgaacc agaagtcttc ttttggagcc
      301 ttcactacta atcacgacaa acaacggctc gccacaagcc atatctagaa ggaatgttgc
      361 cttatgacac ctaacatttc aattactctg aatacaccac atgtcacaat cgaacgttat
      421 agcgaactga ctggcctttc tattgatacg attaacgaca tgttggctga tggccgacta
      481 cctcgtcatc gtcttcgtaa agacaaaaaa cgtgaaaagg taatgattaa cctggctgct
      541 ctgactgttg atgctttgtc tgcttaatag acgtctattt tcgcaataag acgctgagtt
      601 cgattttgcg ataagttcgg aattgaaaac catgtttgat taccaagttt ccaaacatcc
      661 acattttgat gaagcctgtc gtgcattcgc actgcgccac aacctggtgc agctggcagg
      721 acgtgcagga atgaatgtgc agattctgcg aaacaagttg aaccctgctc aacctcattt
      781 attaaccgca ccagaaattt ggctgcttac cgatctgact gaagattcga cgctggtaga
      841 tggctttctg gctcagattc actgtctgcc atgtgtaccg attaatgagg tagcaaaaga
      901 gaaactgcca cattacgtca tgagtgcaac cgcagagatc ggacgtgttg ctgcaggtgc
      961 ggtatctggc gatgtaaaaa ccagtgcagg tcgtcgtgat gctatcagca gcattaactc
     1021 tgtaacacga ctgatggcgc tggctgctgt ttcattgcag gcccgtttac aggctaatcc
     1081 tgcgatggcg agtgcagttg ataccgtgac tggcctcggt gcttcattcg gtttgctgtg
     1141 aggtgcttat gctgacgaaa gaaccatcat ttgcatcgct gctggtaaaa caaagtccgg
     1201 caatgcacta cggtcacggc tggatttatt taccttgtgg gaaaaagtgg catccaagtt
     1261 ttgaattaca accccggcaa caggcttacc ggggaagaaa taaaaagcga ttcctacaaa
     1321 gatttatggt taacgtggca aacaacctgc gaaccaagcg aaaagttttt cttggaagct
     1381 gaggtttaca tcgtcaaccc gaccacaggc aatagcagct cttttgtagg cagggtttaa
     1441 caaagatccg aatgttgtgg agtcagagtg atgcaaagct ttcaattttt gtgcaatgaa
     1501 ctgtggagac aattcatcct cggcgtaaag caactcacca tattgtcggc tttgtacgct
     1561 tgctcgttcc gcaataatag ctggttgcca tacaagttgg attgttgcga ttacaacaac
     1621 aggaaaagcg aacaaccatt ccagacctgt attagcaaat acagctgttc cgcttataag
     1681 ttggattgct gtcattaatt tatcagctcg accatggaac gttgctgtca ttagttcaag
     1741 gtaataacag tagtgtaatt ggaagtattc ggagctttgt ttagtcatgc cggattccta
     1801 tttcttctct tgccccggtt caggctgagg tgctggagcc gggcgaggga gtacatgaaa
     1861 gttgtctgtt tctgacatca gttttctcct ttgaggggta gatgattgtt agcacaaata
     1921 gtctaccact gaggcacgcg ccgggcgtgt agaaaaattc ccggcacaat catttgcatc
     1981 gctgctggta aagcaaagcc cggcaatgca ctacggtcac ggctggatca tgggtgagga
     2041 tggaaaacgc tggcatccat gtcattcaca agatgaattg ctgtctgaat tgaccacgag
     2101 gaaacggaga aagtcaaaat gtatgcagcg gaaagtgaag tggtttatca gtttcgttac
     2161 agaggggaga gttattcagt acctgaagat gatttgctct gttgttatcc gtcgttgtcg
     2221 ggcgatggca gttacttttt cacgctaaaa gatgggacgt ttttacgggg agagcaggtt
     2281 aaagagacga tacgaaaaaa tgtatctcct cttgaacgtt accgtaataa caaacaacga
     2341 tagttgcgtt ttggggatat gaattatggc aattaatggc gctgcggcga ctgttccatt
     2401 aagccccggt gaacgcctga atggacttaa tcacattgcg gagctaaggg cgaaagtttt
     2461 tggcctgaat attgagtcag agcttgagcg gtttattaaa gatatgcgtg atccacggga
     2521 tatcaataat gaacaaaata aacgggcact ggctgccata ttctttatgg caaaaattcc
     2581 agctgaacgt catagcatca gcattaatga gctgaccact gacgaaaagc gggagctgat
     2641 taaagcaatg aatcattttc gtgcagtggt gagcttattt cccagacggc taaccatgcc
     2701 caattaacca actaatgaaa ttaatggcgt aaacccgccg ggcatccctt tatctaaatt
     2761 caggagaatt gattatgcgt aatattgaaa ccctcacgac taaaaccgga ccggatgacg
     2821 cagggcttaa tattttactg acagaggctc gtctggaaga acgccgggca agggctgaag
     2881 caatggcagc tcgccttgat agcctggcgt gtcatatctc atcccgtcag ctaaaccacg
     2941 tggaagcggc agaactgctg cgtgtgactg ctgaagcaat ccagaacgaa gcgcaggaga
     3001 tccactaatg gctgatgcaa tggatctcgt acagcagcgc gttgaagaag aacgccagcg
     3061 ccatatccgt gctgcccgtg ccaaaacgcc gggcgtgtcc cgcgtgcttt gcgttgagtg
     3121 tgaagcgcca attccgccag cacgccgccg tgccattccg ggtgtgcagc tttgcattac
     3181 ctgtcaggaa atcgcagagc tgaaaggcaa acattacaac ggaggtgctg tatgagcacc
     3241 atcctgaaat gggcgggaaa taaaaccgca attatgtccg aactgaaaaa acaccttcct
     3301 gctggcccgc gactggttga acctttcgcg ggttcctgtg ctgtgatgat ggagacggat
     3361 tatcccagct atctggttgc ggatattaat cctgatttaa tcaacctcta taaaaaggtt
     3421 gccgctgatt gtgaatcatt tatatctcgc gccagagttt tatttaagga agcaaacagg
     3481 gaggtggctt attacaacat aaggcaggag tttaattact caactgaaat tactgatttc
     3541 atgaaagcgg tatatttcct gtatctcaat cgtcacggtt accgtggttt atgtcgctat
     3601 aacaagagcg ggcatttcaa cattccctac ggtaattata aaaatccgta tttccctgaa
     3661 aaagaaattc gcgcatttgc agaaaaagcc cagcgggcaa cgtttatctg cgccagcttt
     3721 gatgaaacgc tggcgatgtt gaaggcgggg gatgtggtgt attgcgatcc gccgtatgac
     3781 ggcacgtttt ccggttatca cactgacggc ttcactgaag atgaccagta tcacctggca
     3841 tccgttcttg aacatcggtc atcagaagga catccggtca ttgtttctaa cagtgacaca
     3901 tccctgatcc gttcgctgta tcgcaatttt actcaccact acatcaaggc aaaacgcagc
     3961 atcggcgtag cagctggtga gagtaaatct gcaacagaaa tcatcgctgt ttctggggcg
     4021 tgctgctggg tgggatttga tccttcgcgt ggcgtggata gttcagccgt gtacggagtg
     4081 cgtgcatgag ccatgctgat atgaacaact gcagcggctt taacgaggcc gccgcagcat
     4141 tctcatggaa cagcccgaaa aaggctatta acccttatct ggacccggcg gaagttgcgc
     4201 cggtttctgc gctttcaaac ctgatcactc tgtacgctgc cgataacgag caggaacaac
     4261 tgcgccgcga ggcactgagt gatcaggtct gggagcgtta tttctttaat gaatcccgtg
     4321 atcctgtcca gcgcgaaatg gagcaggata agctcattag ccgggcaaag ttggcgcatg
     4381 agcagcagcg ttttaacccg gacatggtca ttctggcaga cgtcagcgtc cagccctccc
     4441 atatcagcaa gccgctgatg caacgtattg aatacttcag cagcttgggc aggccaaagg
     4501 cttattcccg ctatttacgt gagacgatta agccatgtct ggaacgactg gagcatgtac
     4561 gcgacagcca gctatctgca tcttttcgtt ttatggcaag ccatgtaggg ctggacggcc
     4621 tgctgattct gcctgaaatg agtcaggatc aggtgaaacg cctgtctacc cttgtcgctg
     4681 cgcatatgag catgtgcctt gatgcagctt gtggtgattt gtatgccacc gatgacgtta
     4741 agccggaaga aatccgcaag acatgggaaa aggtggcagc ggaaaccctg cgtctggatg
     4801 tcatcccgcc tgcgtttgag caactccgtc ggaaaagaaa ccgccgtaaa cccgtgccct
     4861 atgaactcat tccgggttcg ctggcgcgta tgttgtgcgc cgactggtgg taccggaaat
     4921 tatggaaaat gcgttgcgaa tggcgggaag agcagttgcg tgctgtctgc ttggtcagca
     4981 aaaaagcatc tccctatgtc agctatgaag ccgtgatgca taaacgtgag cagcgccgca
     5041 agtcactgga gtttttccgt tctcatgaac tggtgaacga agagggcgac acgctggata
     5101 tggaagacgt ggtaaacgcc agcagcagca acccggcgca ccgccgcaat gagatgatgg
     5161 cctgtgttaa aggtctggag cttatcgcgg aaatgcgcgg tgactgcgcc gttttctaca
     5221 ccattacctg tccgtcacgt ttccattcca cgctcaataa cggcagacca aacccaacct
     5281 ggacaaatgc gacggtaaga caaagcagcg attatctggt cggcatgttt gctgcatttc
     5341 gtaaggcgat gcacaaagcc gggttgcgct ggtatggcgt gcgggtggct gagccgcatc
     5401 atgacggtac agttcactgg cacctgttgt gtttcatgcg caaaaaagac cgccgcgcca
     5461 ttactgcatt gttgcgtaag tttgccatcc gtgaagaccg cgaggaactg ggtaataaca
     5521 ctggtccacg ctttaaatct gagctgataa acccgcgcaa aggaacgccg acaagctaca
     5581 tcgcgaaata catcagtaag aacattgacg ggcgtggtct ggctggcgag atcagcaagg
     5641 aaacgggtaa atccctgcgt gataacgctg aatacgttaa tgcctgggcg tctctgcatc
     5701 gtgttcagca attccgcttc tttggcattc cggggcgtca ggcttaccgt gaactgcgat
     5761 tgctggctgg tcaggcggca aggcaacagg gggacaaaaa agcaggtgcg ccggtactgg
     5821 ataacccgcg ccttgatgcc atcctggctg ctgctgatgc tggttgtttt gccacctaca
     5881 tcatgaagca gggcggcgta ctagttcccc gcaaatatca cctcatcaga accgcttatg
     5941 aaatcaacga agagccgacc gcctatggcg atcacggcat tcgtatttat ggcatctggt
     6001 cacccattgc agagggcaag atctgcactc atgcagtgaa gtggaaaatg gttcgtaaag
     6061 ccgttgacgt tcaggaggcg gcagccgacc agggcgcttg cgccccttgg actcgtggca
     6121 ataactgtcc ccttgctgaa aatttgtacc aacaagggaa agacaaatca gctgatggag
     6181 ataccagaac ggatatcacc cgcatggatg acaaggagtt gcacgattac ctgcacagta
     6241 tgagcaaaaa agagcgccgg gaactggctg caaggttacg cctggtgaaa ccgaaacggc
     6301 gtaaagacta caaacagcga attacagacc atcagcgaca gcagctcgtc tatgaactgg
     6361 agtccagagg atttaatggc agcgagaaag aggtcgattt actccttcgc ggcggcagta
     6421 ttccgtcagg agcaggcctg cgtatcttct atcggaacca gcgtttgcag gaagatgata
     6481 agtggcggaa cctgtattaa ttacgctggt taacaattcg tgctcttaat aataccaagt
     6541 atatc
//
LOCUS       DAMZII010000208         6531 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZII010000208 DAMZII010000000
VERSION     DAMZII010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.209"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            16..579
                     /gene="srlA"
                     /locus_tag="POA77_004526"
                     /gene_synonym="gutA"
     CDS             16..579
                     /gene="srlA"
                     /locus_tag="POA77_004526"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDI6367854.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            576..1535
                     /gene="srlE"
                     /locus_tag="POA77_004527"
                     /gene_synonym="gutE"
     CDS             576..1535
                     /gene="srlE"
                     /locus_tag="POA77_004527"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDI6367855.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1546..1917
                     /gene="srlB"
                     /locus_tag="POA77_004528"
                     /gene_synonym="gutB"
     CDS             1546..1917
                     /gene="srlB"
                     /locus_tag="POA77_004528"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDI6367856.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1921..2700
                     /gene="srlD"
                     /locus_tag="POA77_004529"
     CDS             1921..2700
                     /gene="srlD"
                     /locus_tag="POA77_004529"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDI6367857.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2805..3164
                     /gene="gutM"
                     /locus_tag="POA77_004530"
     CDS             2805..3164
                     /gene="gutM"
                     /locus_tag="POA77_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDI6367858.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3231..4004
                     /gene="srlR"
                     /locus_tag="POA77_004531"
                     /gene_synonym="gutR"
     CDS             3231..4004
                     /gene="srlR"
                     /locus_tag="POA77_004531"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDI6367859.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            3997..4962
                     /gene="gutQ"
                     /locus_tag="POA77_004532"
     CDS             3997..4962
                     /gene="gutQ"
                     /locus_tag="POA77_004532"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDI6367860.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4959..6473)
                     /gene="norR"
                     /locus_tag="POA77_004533"
     CDS             complement(4959..6473)
                     /gene="norR"
                     /locus_tag="POA77_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDI6367861.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
BASE COUNT         1495 a         1793 c         1729 g         1514 t
ORIGIN      
        1 ctgaaggaga gaacaatgat agaaaccatt actcatggtg cagagtggtt tatcgggctg
       61 ttccaaaagg gcggagaggt gtttaccggg atggtgaccg gcattcttcc gctgttgatt
      121 agcctgctgg ttatcatgaa cgcactgatt aattttatcg gtcagcatcg tattgaacgt
      181 tttgctcaac gttgcgccgg taaccctgtt tcccgttacc tactgttacc gtgcattggc
      241 acgtttgtct tttgcaatcc gatgacctta agcctgggtc gctttatgcc ggaaaagtac
      301 aaacccagct actacgcggc ggcctcttat agctgccact caatgaatgg cctcttcccc
      361 catatcaacc ctggcgaact gtttgtttat cttggcattg ccagcggtct gacaacgctg
      421 aacctgccac ttggcccact ggcggtgagt tatctgctgg ttggtctggt caccaatttc
      481 ttccgcggct gggtgaccga tctgaccacc gccatttttg agaaaaagat gggcattcaa
      541 cttgaacaaa aagttcacct ggcaggagca acatcatgac gcatattcgg atcgaaaaag
      601 gaacgggtgg ctggggcggc ccgcttgagc tgaaagccac gccgggaaaa aaaatcgtct
      661 atatcaccgc cggtacccgg cctgcgattg ttgacaaact ggcacagctt actggctggc
      721 aggctattga cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa
      781 tcgactgtgg cggcacatta cgctgcggca tctatccgaa acgacgtatt cccaccatta
      841 atatccactc gacgggcaag tccggtccgc tggcgcagta cattgtggaa gatatttatg
      901 tctctggcgt aaaagaagaa aacatcactg tagtgggtga tgcgacacca caaccctctt
      961 ccgtgggccg tgactatgac accagtaaga aaatcaccga acaaagcgat ggtttactgg
     1021 cgaaggtggg aatgggcatg gggtccgccg ttgcggtgct gtttcaatct ggtcgtgaca
     1081 ccatcgacac tgtattaaaa accattctgc cgtttatggc attcgtctcg gcgctcattg
     1141 gcatcattat ggcttctggc cttggtgact ggattgccca cggtcttgct ccgctggcga
     1201 gccatccact gggtctggtc atgctggcgc tcatctgctc cttcccactg ctttcacctt
     1261 tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt attgattggc gtgcagattg
     1321 gtctcggcaa tattccgccg catctggctt taccggcact gtttgccatc aacgcgcagg
     1381 cggcctgcga cttcatcccg gtcggtttgt cgctggcgga agcccgtcag gacacggttc
     1441 gcgtcggtgt cccttctgta ctggtgagcc gctttttaac cggcgcaccg actgtactga
     1501 tcgcctggtt tgtctccggt tttatctatc aatagaggct gaaacatgac cgttatttat
     1561 cagaccacca tcacccgtat cggcgcgagt gccactgacg ccctcagcga ccagatgctc
     1621 atcacctttc gtgaaggcgc gccagcggac ctcgaagagt attgcttcat tcattgccac
     1681 ggcgagttga aaggtgcact ccatcccggt ttgcaattta cgctcgggca gcatcgctat
     1741 ccggtgaccg ccgttggcag cgtggcggaa gacaaccttc gcgaactggg tcatgtcacc
     1801 ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca ctgtccatgt ggcaggccct
     1861 gtacccgacg atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa
     1921 atgaatcagg ttgccgttgt catcggtggt gggcaaacat taggcgcgtt cctgtgccac
     1981 ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca
     2041 aatgtggcac aagaaattaa cgccgaatat ggtgaaagta tggcgtacgg ttttggtgct
     2101 gacgccacta gcgagcaaag cgttctggcg ctctctcgtg gggtagatga aatctttggt
     2161 cgcgtggatt tgctggtcta tagcgccgga atagcaaaag cagcctttat cagcgacttc
     2221 cagctcggcg attttgaccg ttcgctacaa gtgaatctgg tgggttattt cctgtgtgcc
     2281 cgtgaatttt cgcgtttgat gatccgcgac gggattcagg gtcgcattat tcagatcaac
     2341 tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc gaaatttggt
     2401 ggcgtcgggc tgactcaatc actggcgctg gatctggcgg agtacggcat tacggtgcat
     2461 tcactgatgc tcggtaacct gctgaaatcg ccgatgttcc agtcactgtt gccacaatac
     2521 gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacatcga caaagtaccg
     2581 ctcaaacgcg gctgcgatta tcaggacgtg ctgaatatgc tgctgttcta cgccagtcct
     2641 aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt gatgttctga
     2701 ttaacagcgg agatccgtta aggatctccg tgagactata gaatgcctga tgcgctacgc
     2761 tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca
     2821 tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc
     2881 gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca
     2941 gcgggcgctt taaaccgcgg gtcgtggtcg ccatcgcgct ggacgatcag cagcgcatcg
     3001 tcgacacctt gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa
     3061 ttaccggtat gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat
     3121 cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt
     3181 acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc
     3241 gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag
     3301 aattggcgca atactttgac accacaggca caaccattcg caaagatctg gtcattctgg
     3361 aacatgccgg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg
     3421 atccgcctat cgatcataaa acgctcatca acacccacaa gaaagagctg attgcagaag
     3481 ctgccgttag ttttatccat gatggcgatt cgatcattct tgatgctggc agtaccgttt
     3541 tgcagatggt tcccctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc
     3601 atatcgtcaa tgcgctatcc gaactggata acgaacaaac tatcctgatg ccaggcggaa
     3661 cgtttcgtaa aaaatcggcc tcatttcacg gacagctggc agagaatgcc ttcgaacatt
     3721 tcagtttcga taaattgttt atgggcaccg acggcatcga tctcaatgcg ggcgtaacca
     3781 ccttcaacga ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt
     3841 tgatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa
     3901 gcgtcgataa gctgattacc gacgcaggta tcgatccggc gtttcgtcag gcgctggaag
     3961 agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg
     4021 ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc gtttaccgga acgtctgggc
     4081 gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg
     4141 ggaattggca aatcgggcca cattggtaag aaaatcgccg caacacttgc cagtaccggc
     4201 acaccggctt tttttgtcca tccggcagaa gcgctgcacg gcgatctggg gatgattgaa
     4261 agccgcgatg tgatgctgtt tatctcttac tccggtggcg cgaaggaact ggatctgatt
     4321 attccgcgtc tggaagataa atctatcgcg ctgctggcga tgaccggcaa accgacgtca
     4381 ccgctgggcc tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg
     4441 atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg
     4501 atggcggtca tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc ccacccagcc
     4561 ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg tgacgatgcc
     4621 atcccacagg tggcgttaac cgccagcgtg atggatgcga tgctggaact cagccgcacc
     4681 ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac
     4741 ggcgatttac gtcgctggct ggttggcggc ggcgcactca ccacgccagt taatgaagcg
     4801 atgacggtcg gcggcaccac gttgcaatcg caaagtcgcg ccatcgacgc caaagagatc
     4861 ctgatgaagc gcaaaatcac tgccgcaccg gtggtagatg aaaacggcaa actcaccggc
     4921 gcaattaacc tgcaggattt ctatcaggcc gggattattt aatccttcat tcccagacgt
     4981 ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca tccgcgcgca ggcagcccag
     5041 ttgtgatgat tttgtgccag cgcctgacga atagtttcac gctggaacgc ttctgtcgct
     5101 tcacgcaggt tttgcttaac aacgggcacc gccgccactt ctggcgtcgg caacgtcacc
     5161 tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgttgcg ggtggctctc
     5221 gacagcacta ccgcccgatg aatagcatgt tccagttcgc gcacgttccc cggaaaacga
     5281 tagtgttgca gtaaatttcg cgctccggca cttaatacca cgcgggagag ccccagccgc
     5341 aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc
     5401 agcggcggca ccgaaagtgg aaacacgctc aggcgatgaa acaagtcagc gcgaaatcgc
     5461 cctgccagca cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc
     5521 cgcaaactgc ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc
     5581 ttggcctgca atgccaacga caactcgccg atctcatcca gaaacagagt gccgttatcc
     5641 gccatttcaa acttcccgct gcggttactg atagcgccag taaacgctcc tttcacatgc
     5701 ccgaacagtt cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc
     5761 ggattcaccg cccgtggcga ggcttcatga atcgctttcg ccaccagctc cttaccggtt
     5821 cccgtctcac cgctgatcag gacgttgaga tcggacgccg ccacaatctc aatctctttt
     5881 ttcagttgcg tcatgccagg ggacaagcca atcatctgcg tctgtttcac cgcttcaaac
     5941 ggcgtggcat cgcctggcat catattctgg ctttccagtt gttcaatcag caacgcattg
     6001 cttaacgctc ccgccgccag cgcagcaatc agccgtagct cttcgtcgct gaaaacatcg
     6061 aactgatcgg gctgcatccc gtcgagcgtc agtgcgccga tcaggttttg cccggcaaac
     6121 aatggcagac caacgcaggc gtgaaccttc agactctcct gcccaggaat caaaccgtca
     6181 tagggatcgg gcaattcgct gtctgcggga aagcgcacca catccccggc gcgggcaatc
     6241 gcttccagcc gtggatgccc ttccagcgca aagcgtctac cgagtacatc ctttgccaga
     6301 ccgtcgatgg caagcggaat aaactgccgc gaatcgtaac gtagcaacgc agacgcatcg
     6361 cactccagca cctgacgtag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc
     6421 ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc
     6481 tcactgtcaa tttgactata gatattgtca tattgaccat ttgattgata g
//
LOCUS       DAMZII010000209         6402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZII010000209 DAMZII010000000
VERSION     DAMZII010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.210"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..276
                     /locus_tag="POA77_004534"
     CDS             <1..276
                     /locus_tag="POA77_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication-associated protein G2P"
                     /protein_id="HDI6367862.1"
                     /translation="NLPRKIQSTYLLWKQGANMKEMLPKPTFYRHRKELLSFGIDINF
                     YCESPDSNNVVPLVRTLEAKPAKIPSWVYEKGLIFDYNRISHASNWH"
     gene            288..605
                     /locus_tag="POA77_004535"
     CDS             288..605
                     /locus_tag="POA77_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6367863.1"
                     /translation="MSNYGLFVKGKMLGARQRNKVNGQGYYNEIGVGLEIPDGFGGTK
                     QDQIIIRVSQALVNAGVMNQANNFIGKLVQIPVYVRVWSMEGREGVTYNISSDGGITE
                     IKG"
     gene            608..859
                     /locus_tag="POA77_004536"
     CDS             608..859
                     /locus_tag="POA77_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367864.1"
                     /translation="MDTSGFDIQFDNHIPENGYRIEGYLCNANNAKECQAIMVRSEPF
                     HQIDYSAMGNYWTLGFGSVLLLWLFSVGVGQVIKMVRTA"
     gene            894..1127
                     /locus_tag="POA77_004537"
     CDS             894..1127
                     /locus_tag="POA77_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367865.1"
                     /translation="MFKKIVSFLAVPALMAVSGATFAAEGAASSGVDLSPLTNSIDFS
                     TVLVAIMAVAASLVTLYAGVAGVRWVLRTVKSA"
     gene            1192..2904
                     /locus_tag="POA77_004538"
     CDS             1192..2904
                     /locus_tag="POA77_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367866.1"
                     /translation="MEIGFPFMGDRLSLCRYSWAKRLTVYSFIFSFLWVSFPRYSYSF
                     VPAIAAVAARAVIPKVVGRVLVRRFAANDAIYTASQLTATRVFVGRAAANAAEYLPAA
                     SSYKMSGVATWAGIAAAVSSFVPSSLSSSDGSVMVMTNGKKISDNLYEVTYSGQSGES
                     KTITVNFEPQELSPVILHVSRNNVDAGSPVVGVETGYSTPDNALYYYQDSKELIYYYG
                     DNPTEIARNYLNDYNSRTYTETLTNFERTVTNKVVNSNGDVSFTEQNYKFTYPSSFYE
                     IPEITHLYSNPAASSFPAGIPMYENVAGLPMYYSVAYLTAGKQYQYHNTPCKTTNQSN
                     GGYSTICAVPEKEDYTTKDIDEKSELTIWTNTKYKAMTEVLEAGNIESMIDYLEYLDS
                     VSVSPALLADMINELWSEAAVNADYNGLPFKEVSPAEVTSAMSELRLSPTLLDMLSPV
                     SDSAGADVNIDITINNNSGSDTGNNGNIDLGEDPGVKEPELEETPTARDILTPIINLL
                     PFTNEFNIGSRSASCPVVEFSVFNHQYRIDSHCPLIEQNRSAVETIFLIIWGFVALRI
                     ILSA"
     gene            2917..3204
                     /locus_tag="POA77_004539"
     CDS             2917..3204
                     /locus_tag="POA77_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDI6367867.1"
                     /translation="MFGILISALNTLLGFVFRSLIIKFVVFFALYFVVQGFVEILVEL
                     LPDSSNLSSLFANLSDGFWYFINLSKLPQGISMIISAMATRFIIRRIPVIG"
     gene            3208..4218
                     /locus_tag="POA77_004540"
     CDS             3208..4218
                     /locus_tag="POA77_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367868.1"
                     /translation="MAISAYIGIPGSGKSYEAVCNVIIPAFTSGRRVVTNIYGLQKDK
                     ITERYPDATGEIIVVDNDDVLKADFFPFKGGEGSFCQFGDLIVIDEAWRIFGSDKDMT
                     AEKKSFIAEHRHFTHPETGISCDLVIVNQSLSNIARFLKDKIETTYRMRKLKALGLNN
                     HYCIDVYSGHKIYKSNLVTSYRNKYNPDIFELYKSYEGNNGNEKQTDKRQSIWNSGKV
                     RFFLVLFPLMFIGSGWLIYSFFSTFGRSDPSPDLTTTDVRDAAMFRSSAATPSEPAEP
                     PLSTEWRISGRMTSEGRAFVILVNGAGVLRAVPASSFNYKGMLMSGIIDGERVTLYTG
                     KK"
     gene            4218..5462
                     /locus_tag="POA77_004541"
     CDS             4218..5462
                     /locus_tag="POA77_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDI6367869.1"
                     /translation="MKKILLALTLLFSSCAFAGSELELNKVKLPEAISLIYSEVLKVP
                     YMLDPQLVNDERMITFRLTSDIDEREFVTRYLGNMNIRIWTKKGVDFIAPYTPKEPVK
                     PRYTWTYTPQYRSVAYLSDILGGYVSGSFNNSGAVISDDSLKGSSGASNYINRTGDIL
                     VYYGTKEDIAILKTLVTSLDTMSDEVVVSGYVFEVQTSQSDGSGILLAAKILSDKFNI
                     SVGAAGLDNFINIRTGSIDAIFNLLKTDSRFTVVSAPRLRVKNNASASFSVGSDVPVL
                     GSVTVNNNTTTQSVEYRSSGVLFNVTPSIKSRTMDLKIQQQLSNFVTTETGVNNSPTL
                     IKRDVTTEVSLADGDIILLGGLAEQKDSKASSGWSFFGSRTSESNKTDIMVMLQVRKV
                     DRSRATPRSAARSGELFRDNLN"
     gene            5551..5799
                     /locus_tag="POA77_004542"
     CDS             5551..5799
                     /locus_tag="POA77_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367870.1"
                     /translation="MSVKNKARDRLPGGRLKSYRRVGSHFASCARWFDKSPSWYRNMM
                     MTRPERREVRRLLNQVMRGHDADGIAFPVSHRPFVYWW"
BASE COUNT         1715 a         1248 c         1471 g         1968 t
ORIGIN      
        1 aatctcccaa gaaaaataca gtctacatat ttattgtgga agcagggcgc gaacatgaaa
       61 gagatgttac cgaaacctac attctaccga caccgtaaag aattactttc tttcggaata
      121 gatattaact tttattgtga gtcaccggat tctaataacg ttgttccgtt ggttcgtact
      181 cttgaagcca aaccagccaa aattccatca tgggtttatg agaaaggttt aattttcgat
      241 tataaccgta tttcacatgc cagtaactgg cattaaagga gagtgatatg tctaattatg
      301 gtctttttgt aaaaggtaaa atgcttggtg ctcgccagcg taataaggtc aatggtcagg
      361 gatactataa tgaaattggt gttggcttag agattcctga tggttttggc ggcactaagc
      421 aagaccagat tattattcgt gtatctcagg ctcttgttaa tgccggtgta atgaatcagg
      481 caaataattt tatcggtaag ttagtccaga tacctgtata tgttcgcgtc tggtcaatgg
      541 aggggaggga aggagtaact tataacattt catcagatgg cggcattact gaaataaaag
      601 gctgattatg gatacatcag gtttcgatat tcagtttgat aatcatattc ctgaaaatgg
      661 ttaccgtatt gaaggttatt tatgtaatgc gaacaatgca aaagaatgtc aggcaataat
      721 ggtacgttct gaaccatttc atcaaattga ttattctgca atgggaaatt actggacatt
      781 aggttttggc tctgtcctgc tgctctggct tttttctgta ggtgtgggac aggtaataaa
      841 gatggttcgt actgcttgaa tgcgaacctt taaaatgtaa tggagataga gttatgttta
      901 aaaaaatcgt tagttttctg gctgtacctg cattaatggc tgtttctggc gcgactttcg
      961 ctgcagaagg agcggcatca agtggtgttg atttatcgcc gctgacgaac agcattgatt
     1021 tcagtacagt tctggttgcg attatggccg ttgctgcttc actggtaaca ctttatgccg
     1081 gtgtcgctgg cgttcgctgg gtattgcgta ccgttaaatc cgcataaatt cattattcat
     1141 gggcgatgca atatcgcccg ttttacttga ggttattatg gagattgaat tatggaaatt
     1201 gggttccctt ttatggggga tcgtctcagc ttatgtcgtt attcttgggc taagaggctg
     1261 acggtttatt cctttatttt ttcctttctc tgggtttcct ttccccgata ttcatattca
     1321 tttgtaccag caattgcagc agtggctgcg cgggctgtta ttcctaaagt cgttggccgg
     1381 gtgcttgttc gtcgctttgc tgccaatgat gcgatttata cggcatcaca gcttacagca
     1441 acccgtgttt ttgttggccg cgctgcggct aatgctgccg aatacttgcc tgctgcctca
     1501 tcatacaaaa tgagcggtgt tgcaacgtgg gcgggtattg ctgctgctgt atcatctttt
     1561 gttccgtctt ctttgagttc atctgacggc tcggtaatgg taatgactaa cggtaaaaag
     1621 atttccgata atttatatga agtaacgtac agtggtcaaa gtggtgaaag taagacgata
     1681 accgttaatt ttgaaccaca ggaattaagt ccggtaatcc ttcatgttag tcgtaataat
     1741 gtggatgcgg gttctccagt tgtaggtgtt gaaacaggat attccacgcc agataatgca
     1801 ctttattatt atcaggattc gaaagagcta atttattact atggtgataa tccaactgaa
     1861 attgccagaa attatcttaa tgactataac tcgcgtactt atactgaaac actaacgaat
     1921 tttgaacgca ctgtgacgaa taaagttgtc aacagtaatg gagatgtctc ctttactgaa
     1981 cagaattata agtttactta tccttcctca ttttatgaaa ttcctgagat tacacacttg
     2041 tatagcaacc cggccgcatc ttcatttccc gcaggtattc ccatgtatga aaatgtagca
     2101 gggcttccca tgtattacag cgttgcttat ttaacagcag gaaagcaata tcagtatcac
     2161 aacacgcctt gtaaaacgac taatcagtca aatggtggat attcgacgat ttgcgccgtt
     2221 cctgaaaaag aggattacac cacaaaagat attgatgaaa agagtgagct tactatctgg
     2281 accaatacca aatataaagc catgacggaa gtactggagg ccggaaatat tgagtccatg
     2341 attgattatc tggagtatct tgatagtgtt agtgtatcgc cagcacttct tgccgacatg
     2401 attaacgagc tgtggtctga agctgccgtt aatgccgatt ataacggctt accgtttaaa
     2461 gaggtttcac cggctgaagt aacttctgcg atgtcggaac ttaggctatc tccaacgtta
     2521 ttagatatgc tttcacctgt atctgacagt gcaggggctg acgtcaatat tgatattacc
     2581 attaataaca actcaggatc tgacactgga aataatggca atatagattt gggagaagat
     2641 cccggtgtaa aagagcctga gcttgaagaa acgccaacgg caagagatat tttaacgcca
     2701 attattaact tgttgccttt tacaaacgaa tttaacatcg gctctcgttc agcatcctgc
     2761 cccgttgttg aattcagcgt gtttaatcac cagtacagaa ttgactccca ttgtccgtta
     2821 attgagcaga acagaagcgc cgtagaaacc atatttctga ttatatgggg atttgttgcg
     2881 ctccgtatta ttctgagtgc ctaaaggagt gcaactatgt ttgggattct gattagtgca
     2941 ttaaatactt tgttaggttt tgtattccgg tcattgatta ttaaattcgt cgtattcttt
     3001 gcgctgtatt ttgtagtcca gggcttcgtt gaaatccttg ttgagttact gccagattca
     3061 agcaatctct catcgctgtt tgccaattta tcagacggat tctggtactt cattaacctg
     3121 agtaaattac cacaggggat tagtatgatt atctccgcaa tggctactcg ttttattatt
     3181 cgacgtattc ctgttatagg gtgagttatg gctatttctg catatattgg catacccggc
     3241 tcaggaaaaa gttatgaagc cgtttgcaat gtcattattc cggcatttac cagcggccgg
     3301 agagttgtga cgaacattta tggtttacaa aaagataaaa tcaccgaacg ttatcctgat
     3361 gcaacgggag aaattattgt tgtggataat gatgatgtgc ttaaagcaga tttctttcct
     3421 tttaaaggtg gggaagggag cttttgccag tttggtgatt taattgttat tgatgaagca
     3481 tggcgaatct tcggtagcga taaggatatg acggctgaga agaaatcatt tattgctgaa
     3541 catcgtcatt ttacgcaccc tgaaacgggt attagctgtg atttggttat tgtaaatcag
     3601 tcactttcta atattgctcg ctttctgaaa gacaaaatag aaacaactta ccggatgcgc
     3661 aagctgaaag cgttgggcct gaataatcat tactgcattg acgtatattc aggccacaaa
     3721 atctataaaa gcaacctcgt caccagttat cgcaataaat ataaccctga tatttttgaa
     3781 ctttacaaaa gctatgaagg aaataacggt aatgaaaagc agacagataa acgccagagc
     3841 atctggaatt ctggcaaagt caggttcttt cttgtgcttt ttccattgat gtttatcggg
     3901 tcaggctggc tgatttactc atttttcagc acgtttggcc gaagcgatcc ctcgccagat
     3961 ttgactacaa cagatgtacg tgatgcggcc atgtttcgtt cttccgctgc tactccctca
     4021 gaaccagctg aaccgccact ttcaaccgag tggcgtatat cagggagaat gaccagtgaa
     4081 ggcagggcgt ttgtgattct tgttaacggt gccggtgttt tgcgtgccgt tcctgcatcc
     4141 agttttaatt ataaagggat gttgatgagc ggaattattg atggtgagcg tgtgacgctt
     4201 tatacgggga aaaaataatg aaaaagattt tactcgcatt aacgctacta ttttcttcat
     4261 gtgctttcgc agggtcagag cttgaattaa ataaagtcaa gctaccggag gctatttctc
     4321 ttatttacag tgaggtgctt aaagtcccgt atatgctaga tccacagctt gttaatgatg
     4381 aacgaatgat tacattcagg ttaacatctg atattgatga acgggaattt gtaacccgtt
     4441 atcttggcaa tatgaacatt cgaatatgga cgaaaaaggg tgttgatttt atcgcgccct
     4501 atacgccgaa agagccggtt aagccacgtt atacatggac ttatacgcct cagtaccgtt
     4561 ctgttgccta cctgtctgat attcttggcg gctacgtttc aggctccttc aataacagtg
     4621 gagccgtgat ttctgacgat tcgctaaaag gttcatcagg ggcaagcaac tacatcaatc
     4681 gaaccggtga tattctggtt tattatggta cgaaagagga tatcgccatc ctgaaaacac
     4741 tggtcacttc gctggatacg atgagtgatg aagtggttgt ttctggttat gtttttgagg
     4801 ttcagacctc gcagtctgac ggctccggca ttcttttagc ggctaaaatt ctgtctgata
     4861 aattcaatat ttcagttggt gctgccggac tggataattt tatcaatatt cgaaccggct
     4921 ccattgatgc cattttcaat ctgctgaaaa ccgacagtcg ttttactgtt gtcagtgcgc
     4981 cacgactgcg ggtaaaaaat aatgcctcag cgtctttttc agtcggctct gatgtgccag
     5041 tactgggcag tgttacggtg aataacaata cgacaacgca atccgttgaa tatcgttcca
     5101 gcggtgtttt gtttaacgtg acgccatcaa tcaaaagtcg gacaatggat ctcaagattc
     5161 agcagcagct ttccaacttt gtgaccactg aaaccggcgt caataactcg ccaaccctta
     5221 tcaagcgtga tgtaacaacc gaagtcagcc ttgcagatgg tgatattatt ttacttggtg
     5281 gccttgctga acagaaagac agtaaggcca gttccggctg gagcttcttc ggttcccgta
     5341 ccagtgaaag caacaagact gatattatgg tgatgcttca ggtcagaaag gttgaccgga
     5401 gcagggcgac gccccgcagc gccgcgagga gcggtgaact gttccgggac aacctgaact
     5461 gattgtatgg atttttatta tggcgttaac ttttatgggg tatgaacagt acgacatctt
     5521 ctaggtattc agttacttag aggatttatt atgtctgtaa aaaataaggc gcgtgaccga
     5581 cttcccggtg gccgccttaa gtcttatcgt cgtgtaggtt cacattttgc cagttgtgcc
     5641 agatggtttg ataaatcgcc gtcctggtat cgcaatatga tgatgaccag gcctgagcgc
     5701 cgtgaagtca gaaggcttct caatcaggta atgcgtggtc atgatgctga cggcattgct
     5761 tttccggtca gtcatcgtcc gtttgtttat tggtggtgaa tttcgtacgc acgaaatttc
     5821 acgcataaac accagacaaa aaatcgttaa ctgtctgttt tgaaagataa cgccggtcag
     5881 tgacaaaact tgttttgttg ctgtccgggg ttggccaagc cgtacagttt ccattttcag
     5941 cgttatggct gattcggcgc ggcagcattc atcgggcctt atcatggaaa aattcatgac
     6001 acatcaggag catggtgatg gcactgaaga agtggtgttt gattctcgtg tatcaggtac
     6061 agatttacca actaactgga tgaacaggcc tgattggtga ggtcaggccg ttcacttact
     6121 gctgacagac atttgattct gattcgtttg gagaaacgga tgattttgaa tcactgtcga
     6181 tagtagccag ttgacggcca gtgtcaacga ttcccgccag attctgccat ccttaccatt
     6241 ggtgcgcata atgtcaacgc gttatgttga aaaggccgct gcgaaaatcg aatcccgcag
     6301 cggcctcttt agcataacgt cattgtgcga accaatagct actttttcgt ataactctct
     6361 tcgcatttag ccaaaaacag cagattagta gcggttaaat ga
//
LOCUS       DAMZII010000210         6397 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZII010000210 DAMZII010000000
VERSION     DAMZII010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.211"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            320..685
                     /locus_tag="POA77_004543"
     CDS             320..685
                     /locus_tag="POA77_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6367871.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            725..1420
                     /locus_tag="POA77_004544"
     CDS             725..1420
                     /locus_tag="POA77_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDI6367872.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1487..2905
                     /gene="dcm"
                     /locus_tag="POA77_004545"
     CDS             1487..2905
                     /gene="dcm"
                     /locus_tag="POA77_004545"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDI6367873.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2886..3356
                     /gene="vsr"
                     /locus_tag="POA77_004546"
     CDS             2886..3356
                     /gene="vsr"
                     /locus_tag="POA77_004546"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDI6367874.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3345..4265)
                     /gene="yedA"
                     /locus_tag="POA77_004547"
     CDS             complement(3345..4265)
                     /gene="yedA"
                     /locus_tag="POA77_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDI6367875.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAAVIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4438..5355
                     /locus_tag="POA77_004548"
     CDS             4438..5355
                     /locus_tag="POA77_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDI6367876.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5434..5616
                     /locus_tag="POA77_004549"
     CDS             5434..5616
                     /locus_tag="POA77_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDI6367877.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5787..>6397
                     /locus_tag="POA77_004550"
     CDS             5787..>6397
                     /locus_tag="POA77_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="catalyzes the formation of cyclic
                     di-3',5'-guanylate from guanosine triphosphate; involved
                     in the regulation of cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDI6367878.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVT"
BASE COUNT         1611 a         1519 c         1778 g         1489 t
ORIGIN      
        1 agggggtgtc aatgattgtt gcgctaaacg gctggtgtga tcacgcgcac attaatgaag
       61 gcataaaaaa aagccggcat catgccggcc caaaaaatat aaacaagacg ataaaaggct
      121 aataacgtaa gcatcatgac acagaataat aaaaaggtat aattgtaatc agtgctaata
      181 tattgattga atgaattaat aaagccgata tcaaagataa ataaaatcgc tcaaaacgat
      241 ttttcctgta ttcactgccg ttgcgcaaaa tttatctatt tgttcaaaaa atgattgagt
      301 cttgactggc tcattcaatg tggaaaaatg tgacttttat cacataatag tactaagtct
      361 gaattttccg ggttatctca aaatggaata cggttcgaca aagatggaag agaggctctc
      421 tcgcagccct ggtggcaaac tggctttgtg ggctttctat acatggtgtg gctatttcgt
      481 ctgggcaatg gcgcgttaca tctgggtgat gagccggatc cccgatgccc ccgtgtcggg
      541 atttgaaagc gatttaggct ctaccgcggg aaagtggtta ggggcactgg tcggattttt
      601 atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacaccc gacctcgtcc
      661 cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg gcaacggagc
      721 gcgtatggac ttacaacact ggcaggcaga gttcgaaaac tggttgaaaa accatcatca
      781 gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta cggcacagaa
      841 gctggcggca gatgatgacg tcgatatgct ggtgatttta accgcctgtt attttcacga
      901 tattgtgagc ctggcgaaaa atcatccgca acggcagcgt tcttcaatcc tggcggcaga
      961 agagacgcgt cgcctgctcc gtgaagagtt cgtgcaattt ccggcggaga aaattgaggc
     1021 cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa atcgccccct taaccacgga
     1081 agctaaaatt gtccaggatg cggatcggct ggaagccctg ggggcgattg gcctggcgcg
     1141 cgtgtttgcc gtttcaggag cattgggcgt ggcgctgttt gatggtgaag atccgttcgc
     1201 acagcatcgc ccgcttgatg ataaacgcta cgcgctggat catttccaga ctaagttgct
     1261 gaagctaccg cataccatgc aaaccataag aggcaagcag ctggcgcagc acaacgcgca
     1321 ttttttagtc gagtttatgg cgaagctcag tgccgaactg gcgggggaga atgaaggtgt
     1381 cgatcacaag gtgatagatg cgttttcacc cgcgggataa gcgcgtggcc ctaaatggct
     1441 gtaattatgt taacctgtcg gccatctcag atggccggtt aaatctatgc aggaaaatat
     1501 atcagtaacc gattcataca gcaccgggaa tgccgcacag gcaatgctgg agaaactgct
     1561 gcaaatttat gatgttaaaa cgctggtggc gcagcttaat ggtgtgggtg agaatcactg
     1621 gagcgcggca attttaaaac gtgcgctggc gaatgactcg gcatggcatc gtttaagtga
     1681 gaaagagttc gcccatctgc aaacgttgtt acccaaacca ccggcacatc atccgcatta
     1741 tgcgtttcgc tttatcgatc tatttgccgg aattggcggc atccgtcgcg gttttgaatc
     1801 gattggcgga cagtgcgtgt ttaccagcga atggaacaaa catgcggtac gcacttataa
     1861 agccaatcat tattgcgacc cggcgacgca tcattttaat gaagatatcc gcgacatcac
     1921 cctcagccat aaagaaggcg tgagtgatga ggcggcggcg gaacatattc gtcaacacat
     1981 tcctgaacac gatgttttac tggccggttt cccttgtcag ccattttcgc tggctggcgt
     2041 atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc tgcgatactc agggcacgct
     2101 gttctttgat gtggtgcgca ttatcgacgc gcgtcgtccg gcgatgtttg tgctcgaaaa
     2161 cgtcaaaaac ctgaaaagcc acgaccaggg taaaacgttc cgcatcatca tgcagacgct
     2221 ggacgaactg ggctatgacg tggctgatgc agaagataac gggccggacg atccgaaaat
     2281 catcgatggt aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg tgggttttcg
     2341 tcgcgatctg aatctgaaag ccgattttac cctgcgtgat atcagcgaat gtttccctgc
     2401 gcagcgagtg acgctggcgc agctgctgga cccgatggtc gaggcgaaat atatcctgac
     2461 gccggtgctg tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc gcggtaacgg
     2521 cttcggttat ggaatggttt atccgaacaa tccgcaaagc gtcacccgta cgctgtctgc
     2581 gcgttattac aaagatggcg cggaaatttt aatcgatcgt ggctgggata tggccacggg
     2641 tgagaaagac tttgacgatc cgctgaatca gcaacatcgt ccacgtcggt taacgcctcg
     2701 ggaatgcgcg cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc gcattccggt
     2761 ttcggacact caggcctatc gccagttcgg taactcggtg gtcgtgccgg tctttgccgc
     2821 ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc agcaagaggc
     2881 acaacatggc cgacgttcac gataaggcca ctcgcagcaa aaatatgcgc gcgattgcca
     2941 cgcgtgatac ggcgatagag aagcgcctcg ccagtctgtt aaccgggcag ggcctggcgt
     3001 ttcgcgttca ggacgccagt ctgcccggac gcccggattt tgtcgttgat gaatatcgct
     3061 gcgtgatatt tacccacggc tgcttctggc atcatcatca ctgctatctg tttaaagtgc
     3121 ctgcgactcg aaccgagttc tggctggaga agataggtaa aaatgttgag cgcgatcgcc
     3181 gcgatatcag tcgcttgcag gaactcggct ggcgcgtatt gattgtctgg gagtgcgcgt
     3241 tacgtgggcg cgagaagctg acggatgaag cgcttagcga gcgtctggaa gagtggatct
     3301 gcggcgaagg tgccagcgcg cagatcgaca cgcaggggat tcatttactc gcttgatgcg
     3361 tcctgaataa ctggcgcaac tacgggtttt gccggaaaga gatattttcc caacgtgacc
     3421 agcaccaccg cgaagacaat tacgccgagc gccagccatt caatcttcga cagcgtctct
     3481 ccgcccagtc ccgtacccag caagaccgcg accaccgggt taacgtaggc gtagctggtg
     3541 gcgagagccg gactgacatt acggattaaa tacatataag cgttgatggc gataatcgaa
     3601 ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa gggaagggag cgccgtcagt
     3661 ttttcacctg caatcatcga cgcgatcatt aacaccacgc ctgccgccag catctcaata
     3721 gcacccgcca tcatccctac aggtaaggta atgcgcgagc cataaaccga gccaaacgcc
     3781 cagctaatcg agccgattaa aatcagaatc gcgccccacg gattgccgct taaatttcca
     3841 ccgctattga gcatgatgat tccggcaagc ccaatggcga tacccaccca ttccagtttg
     3901 cgcgttttaa tgccaaacag gcggctgaag cacagggtaa agaggggcac ggttgcaacc
     3961 acaacggcgg cgatgccgga aggaacattt tgatgctcgg caaccgttac catgccatta
     4021 ccgacagcca gcaataacag gccaatcacc gcggcattga gtagcggacg tagcgggggg
     4081 agtttgtgtc cgcgcagtag caaaaatgcc agcaataaaa taccggctgc caggaatcga
     4141 acgcccgcca tcattaacgg aggccagctt tccacgccaa tccgaatgac aaaataggtt
     4201 gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa ctggcggaaa
     4261 cgcataatcc ctcacggtgg aaataaaaag gtggttcata gtaaacgtga aaatcattct
     4321 gctggcgaga gatataattg cacttgattg ttaaaaaaat gttgacctgt gaagcaagta
     4381 tcagagctgc cgtttttgct tcatacttac acccttcaac aataaaaatg agagggaatg
     4441 cttttggccg ggagtagttt actgacgttg ctcgatgata tcgccacact actggacgat
     4501 atctccgtga tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg ggatgactta
     4561 tcgctcaatg cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc cgtggtctgg
     4621 ggcgtggcga aaggatcgct gattaataaa gtgattctgg tgccgctggc gctgatcatc
     4681 agtgcgttta tcccgtgggc gattacgcct ctgttgatga ttggtggcgc gtttctctgc
     4741 tttgaaggag tagagaaagt gctgcatatg ctggaggcgc gtaaacataa agaagatccg
     4801 gcgcagagcc agcagcgtct ggagaagctg gcggcgcagg atccgctgaa gtttgaaaag
     4861 gacaaaataa aaggggcgat tcgtaccgat tttatattgt ctgccgaaat cgtcgccatc
     4921 acgctgggga ttgtggccga agcgccgttg cttaatcagg tgctggtgct ttcaggcatc
     4981 gcgctggtag tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa gattgatgac
     5041 ctggggtatt ggctggcgga aaaatccagc gcgctgatgc aggcattagg taaagggtta
     5101 ttgatcattg cgccctggtt gatgaaggcg ttatcgattg tcggcacgct ggcgatgttc
     5161 ctcgtcggcg gtgggattgt ggtacatgga attgcgccgc tgcatcacgc cattgaacat
     5221 ttcgccgggc agcaaagtgc agtggtggcg atgatattac cgactgtttt aaatctgatt
     5281 cttggattta tcatcggcgg catcgtggtg ctgggagtga aagccgtagc gaaaatacgc
     5341 ggtcaggcac attaatactc gcacaggcta ttatgcaaaa ttcgccatct tcaactaagg
     5401 tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct ttatggttag tgaggaagtt
     5461 acggttaaag agggcggccc gcggatgatt gtcaccggat actccagcgg tatggttgag
     5521 tgtcgttggt atgacggtta cggggtcaag cgggaagctt ttcatgaaac cgagcttgtt
     5581 ccgggggagg ggagtcgttc tgcggaagaa gtttgaaagg gtatcattaa caccaggccg
     5641 gatcacatcc ggcctggtgt gccaatgcct gcaatgaatg atagtcgcgt cttatcatgc
     5701 caccattata aaattatcgc tgttttgcgg tacgctatcg ccaggttatg ccagaatcat
     5761 aaaaaagcag gttgggagtc gtcagggtgc agcacgagac aaaaatggaa aaccagagct
     5821 ggttgaaaaa actcgcacgc cgcctggggc ctggtcatgt cgttaatctc tgctttatcg
     5881 tggtattgct tttttccacc ttgctcacct ggcgtgaagt agtggtgctg gaagatgcct
     5941 atatctccag ccagcgtaat catctggaaa acgtagccaa cgcgctcgat aagcatttgc
     6001 agtataacgt cgacaaactg atctttttgc gtaatggcat gcgcgaagct ctcgtagcgc
     6061 cactggattt cacttcactg cgtaatgctg taaccgagtt cgaacagcat cgcgacgagc
     6121 acgcctggca aattgaactc aaccgacggc gcactctgcc agttaatggt gtgtcggatg
     6181 cattagtcag cgaggggaat ctcctgtctc gcgaaaatga aagcctcgac aatgaaatta
     6241 ccgctgcact ggaagttggt tacttgctgc gactggcgca caactcctcg tcgatggttg
     6301 aacaggcgat gtatgtctcg cgtgccggat tttacgtttc gacgcagccg accttgttta
     6361 cgcgcaatgt tccaacgcgt tattacggct atgtcac
//
LOCUS       DAMZII010000211         6279 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZII010000211 DAMZII010000000
VERSION     DAMZII010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.213"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            39..968
                     /gene="pfkB"
                     /locus_tag="POA77_004551"
     CDS             39..968
                     /gene="pfkB"
                     /locus_tag="POA77_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDI6367879.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1069..1359
                     /gene="ghoS"
                     /locus_tag="POA77_004552"
     CDS             1069..1359
                     /gene="ghoS"
                     /locus_tag="POA77_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI6367880.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1465..2325
                     /locus_tag="POA77_004553"
     CDS             1465..2325
                     /locus_tag="POA77_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6367881.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2366..2902)
                     /locus_tag="POA77_004554"
     CDS             complement(2366..2902)
                     /locus_tag="POA77_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDI6367882.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3049..3717
                     /gene="hxpB"
                     /locus_tag="POA77_004555"
     CDS             3049..3717
                     /gene="hxpB"
                     /locus_tag="POA77_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDI6367883.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3880..4470
                     /locus_tag="POA77_004556"
     CDS             3880..4470
                     /locus_tag="POA77_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6367884.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4603..5994
                     /gene="tcyP"
                     /locus_tag="POA77_004557"
     CDS             4603..5994
                     /gene="tcyP"
                     /locus_tag="POA77_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDI6367885.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(5998..>6279)
                     /locus_tag="POA77_004558"
     CDS             complement(5998..>6279)
                     /locus_tag="POA77_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367886.1"
                     /translation="NINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1541 a         1549 c         1576 g         1613 t
ORIGIN      
        1 ttcggtgcca gactgaaatc agcctatagg aggaaatgat ggtacgtatc tatacgttga
       61 cacttgcgcc ctctctcgat agcgcaacaa ttaccccgca aatttatccc gaaggaaaac
      121 tgcgctgtac cgcaccggtg ttcgaacccg ggggcggcgg catcaacgtc gcccgcgcca
      181 ttgcccatct tggaggcagt gccacagcga tcttcccggc gggtggcgcg accggcgaac
      241 acctggtttc tctgttggcg gatgaaaatg tccccgtcgc tactgtagaa gccaaagact
      301 ggacccggca gaatttacac gtacatgtgg aagcaagcgg tgagcagtat cgttttgtta
      361 tgccaggcgc ggcattaaat gaagatgagt ttcgccagct tgaagagcaa gttctggaaa
      421 ttgaatccgg ggccatcctg gtcataagcg gaagcctgcc gccaggtgtg aagctggaaa
      481 aattaaccca actgatttcc gctgcgcaaa accaagggat ccgctgcatc atcgacagtt
      541 ctggcgaagc gttaagtgca gcactggcga ttggtaacat cgagttggtt aagcctaacc
      601 aaaaagaact cagtgcgctg gtgaatcgcg aactcaccca gccagacgat gtccgcaaag
      661 ccgcgcagga aatcgttaat agcggcaagg ccaaacgggt tgtcgtttcc cttggtccac
      721 aaggagcgct gggtgttgat agtgaaaact gcattcaggt ggtgccacca ccggtgaaaa
      781 gccagagtac cgttggcgct ggtgacagca tggtcggcgc gatgacactg aaactggcag
      841 aaaatgcctc tcttgaagag atggtccgtt ttggcgtagc tgcggggagt gcagccacac
      901 tcaatcaggg aacacgtctg tgctcccatg acgatacgca aaaaatttac gcttaccttt
      961 cccgctaaca aaaacattcc cccagcattg ggggaatcac caccaacctg tcggcaacgc
     1021 gtttctccga ctatgctcaa aagtcatgtg ataacaaagg ggtgaactat ggccagtggc
     1081 gatcttgtcc gttatgtcat aaccgtcatg ttgcatgagg atacattgac tgaaattaac
     1141 gagttgaata attacctgac tcgcgacggt tttttgctca ccatgacgga tgatgaggga
     1201 aatatccatg agctggggac taacactttt ggacttatca gtacccaaag tgaagaagaa
     1261 attagagaac tggtttcggg gcttacccaa agtgcaaccg gcaaagatcc tgaaatcacc
     1321 atcacgacct gggaggaatg gaatagcaac agaaaataaa tggtttttgg gcaataatca
     1381 gtctgtggtg tgcgttagct cgtgttttta caccgcattc ttgcgctaac cttatgatct
     1441 ggcagacaac atgggagaga catcatgtgg caggcaatca gtcgtctttt gagcgagcag
     1501 ttaggtgaag gcgaaatcga actgcgtaat gaactgcctg gcggagaagt ccatgccgca
     1561 tggcatttgc gctatgcagg acatgacttt ttcgtcaaat gtgatgaaag ggaactgctt
     1621 cccggtttta ccgccgaagc cgaccaactg gagttactgt cgcgtagtaa aaccgtcacc
     1681 gtgcctaagg tttgggcagt aggcgctgac cgtgactaca gttttctggt gatggattat
     1741 ctcccacctc gtccgctgga tgcgcatagc gcatttattc ttggtcagca aattgcgcgt
     1801 ttacatcaat ggagtgacca accacaattt ggcctcgatt ttgataactc gctctccaca
     1861 actccacagc ccaacacctg gcaacgtcgc tggtcaacgt tttttgctga acaacggatt
     1921 ggctggcagt tggaactggc agcagagaaa gggatcgctt tcggcaatat cgacgccatc
     1981 gtcgagcata ttcagcagcg tctggcctcg catcaaccgc agccttctct gttgcacggc
     2041 gatttatggt ccggcaactg tgcgctgggt ccggatggcc cgtacatttt cgaccctgcc
     2101 tgctactggg gtgaccgaga gtgcgacctg gcgatgttac cgctgcatac tgaacagccg
     2161 ccacaaatct atgacggcta tcagtcagta tccccgctac ctgccgattt ccttgaacgt
     2221 caaccggttt accaactcta cacgctttta aatcgtgcaa gattatttgg cggtcagcat
     2281 ttggttattg ctcagcagtc attggataga ttattagcag catgatatgg gttgaggata
     2341 atggccgctc cgtgcggcct tttgattaaa taaacccaag cagagaaaag aacacataac
     2401 ctgcagcaat acaaataacc ggcaggatat atagtgagaa aaactgcagg aaaatagtat
     2461 ggtgcggaac aacaatacgg gactcaattt gttcacgcgt tagcccctct tcccctttgg
     2521 ctttttccag aatgagttga tcttcaacac cttcccgcaa gaagcgcgcc tggcgactca
     2581 tccgggcacc ggaatcctgc aacgccagtc cgacaaaaat caggatgaaa atcacccaga
     2641 acataacgtt cacgccacca ttaaaattgg gcgtcgggga gttataccag aacagattca
     2701 aaaaaggcgt attcgcctgc atcatatcga tcatgacatg agtaaagtcg agcatgaccg
     2761 cattaatgcc ttcctggttt tcctgccggg tattcataaa tttcagcaaa gaaatcaatg
     2821 tagagatcaa tgcaggtata aaaatcaccc aacccaaaat ccttttcaaa acagcaatgc
     2881 gtccagcttg ttgatacgtc atgagttctc cttgattaag acgcgtcgtt tcacttagtt
     2941 tacctgtaga tatctgtttt cgcccattct ttaaaggcga tatgataggc gcttaatcat
     3001 aagcacggct taatacctta cacataatgc tctaaaggag aggttgttat gtcaaccccg
     3061 cgtcagattc ttgctgcaat ttttgatatg gatggattac ttatcgactc agaaccttta
     3121 tgggatcgag ccgaactgga tgtgatggca agcctggggg tggatatctc ccgtcgtaac
     3181 gagctgccgg acaccttagg tttacgcatc gatatggtgg tcgatctttg gtacgcccgg
     3241 caaccgtgga atgggccaag ccgtcaggaa gtagtagaac gggttattgc ccgtgccatt
     3301 tcactggttg aagagacacg tccattatta ccaggcgtgc gcgaagccgt tgcgttatgc
     3361 aaagaacaag gtttattggt gggactggcc tccgcgtcac cactacatat gctggaaaaa
     3421 gtgttgacca tgtttgactt acgcgacagt ttcgatgccc tcgcctcggc tgaaaaactg
     3481 ccttacagca agccgcatcc gcaagtatat ctcgactgcg cagcaaaact gggcgttgac
     3541 ccactgacct gcgtagcgct ggaagattca gtaaatggca tgatcgcctc taaagcagcc
     3601 cgcatgcgtt ccattgtcgt tcctgcgcca gaagcgcaaa atgatccacg ttttgtatta
     3661 gcaaacgtca aactctcatc gctgacagaa ctcaccgcaa aagaccttct cggttaatga
     3721 ccaggggcag tgatctcgct gcccctggtt ctttatctga atttgcattc aactgacgga
     3781 ttaatcgtca atttaagaga aagagttaca ccgtcaccac ttccgtgcac tgtataaaaa
     3841 tcctatactg tacgtatcga cagtttagtg agttttatca tgacggcgga aggtcacctt
     3901 ctcttttcta ttgcttgtgc ggtatttgcc aaaaatgccg agctgacgcc cgtgctggca
     3961 cagggtgact ggtggcatat tgtcccttcc gcaatcctga cgtgtttgtt accggacatc
     4021 gatcacccaa agtcgtttct tgggcagcga ttaaagtgga tatcaaaacc gatcgcccgc
     4081 gcttttgggc atcgtggttt tacccacagt ctgctggcgg tatttgcgct gctggcaacc
     4141 ttttacctta aggttcctga aagctggttc attccggctg atgcgctaca aggaatggtg
     4201 ctgggttatt taagccacat acttgccgat atgctgacac ccgccggtgt tcccctgctc
     4261 tggccatgtc gctggcgttt ccgcttgcct atcctggttc cccaaaaggg caaccaactg
     4321 gaacgtttta tctgcatggc attatttgtc tggtcggtat ggatgcccca ttcattaccc
     4381 gaaaacagcg cggttcgttg gtcatcgcaa atgatcaata ccttgcagat ccagtttcat
     4441 cggcttatta agcatcaggt tgaatactaa aaaggcaaaa atcacctttc tggaataagc
     4501 aattccattt gaatataaga gccagctcac agttctgtta atcttgcgcc aacactatga
     4561 ctgctacgca gtgatagaaa taataagatc aggagaacgg ggatgaactt tccattaatt
     4621 gcgaacatcg tggtgttcgt tgtactgctg tttgcgctgg ctcagacccg ccacaaacag
     4681 tggagtctgg cgaaaaaagt gctggtgggt ctggtgatgg gtgtggtttt tggccttgcc
     4741 ctgcatacca tttatggttc tgacagccag gtacttaaag attctgtaca gtggtttaac
     4801 atcgttggta acggctatgt tcaactgctg caaatgatcg ttatgccgtt agtcttcgcc
     4861 tctattctga gcgcggttgc ccgtctgcat aacgcatctc agttaggcaa aatcagtttt
     4921 ctgaccatcg gtacgctttt gtttaccaca ctgattgcgg cgctggtcgg tgtgctggtc
     4981 accaacctgt ttggtttgac ggctgaaggt ctggttcagg gtggtgcaga aactgcacgt
     5041 ctgaacgcca tcgaaagtaa ctatgttggt aaagtctctg acctgagcgt tccgcagctg
     5101 gtcttgtcct ttatcccgaa aaacccgttt gccgatctga ccggagccaa tccgacgtca
     5161 attatcagcg tggtaatttt tgccgcattc ctcggtgtag ctgcgctgaa actgctgaag
     5221 gatgatgcgc cgaaaggtga acgcgtctta accgctatcg ataccctgca aagctgggtg
     5281 atgaaactgg ttcgcctggt catgcagttg accccttacg gcgttctggc actaatgacc
     5341 aaagtggttg caggttctaa cctgcaagac atcatcaaac tgggaagttt cgttgtcgcg
     5401 tcctacctcg gtctgctgat tatgtttgca gtgcatggca ttctgctggg cattaatggc
     5461 gtgagtccgc tgaagtactt ccgtaaggta tggcctgtgc tgacgtttgc ctttaccagc
     5521 cgttccagtg ctgcgtctat cccactgaat gtggaagcac aaacgcgtcg tctgggcgtt
     5581 cctgaatcca tcgccagttt cgccgcctct ttcggtgcga ccattggtca gaacggctgc
     5641 gccggtttgt atccggcaat gctggcggtg atggttgcgc ctacggttgg cattaacccg
     5701 ctggacccga tgtggattgc gacgctggtc ggtattgtta ccgttagttc cgcaggcgtt
     5761 gccggtgtcg gtggtggtgc aactttcgcc gcactgattg tgctgcctgc gatgggcctg
     5821 ccagtaaccc tggtggcgct gttaatctcc gttgaaccgc ttatcgacat gggccgtacg
     5881 gcgctaaacg ttagtggctc gatgacagct ggcacgctga ccagccagtg gctgaagcaa
     5941 accgataaag ccattctgga tagcgaagac gacgccgaac tggcacaccg ttaattctta
     6001 tgctggcaac ggtccgtttt gtataggggc cgttgcttta ctttttaaat gttccatcgc
     6061 ggcgatgcat actgactcat atttaaacag atcgtcagcg aaaaaaatgt ccaacttatc
     6121 aatacattcc tggctcaatg aatgtgcctg tagtgagaag cttaaaacct catacccgag
     6181 acgttttcta agtgaactat ctggaatact cataatatct tcaagcacac cacttcccat
     6241 tcccggaaga tttagtggat taaaaagaag attgatatt
//
LOCUS       DAMZII010000212         6153 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZII010000212 DAMZII010000000
VERSION     DAMZII010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.214"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..63)
                     /locus_tag="POA77_004559"
     CDS             complement(<1..63)
                     /locus_tag="POA77_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367887.1"
                     /translation="MTRQEELAAARAALHDLMTGK"
     gene            complement(60..1985)
                     /locus_tag="POA77_004560"
     CDS             complement(60..1985)
                     /locus_tag="POA77_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDI6367888.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPLLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKSIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETSFSLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(1960..2505)
                     /locus_tag="POA77_004561"
     CDS             complement(1960..2505)
                     /locus_tag="POA77_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDI6367889.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2894..3127
                     /locus_tag="POA77_004562"
     CDS             2894..3127
                     /locus_tag="POA77_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDI6367890.1"
                     /translation="MSTKNRTRRTTIRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQIRPD"
     gene            3184..3594
                     /locus_tag="POA77_004563"
     CDS             3184..3594
                     /locus_tag="POA77_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDI6367891.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(3944..4465)
                     /locus_tag="POA77_004564"
     CDS             complement(3944..4465)
                     /locus_tag="POA77_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDI6367892.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            complement(4670..5107)
                     /locus_tag="POA77_004565"
     CDS             complement(4670..5107)
                     /locus_tag="POA77_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDI6367893.1"
                     /translation="MNRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAASCAKSKTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(5104..5601)
                     /gene="rrrD"
                     /locus_tag="POA77_004566"
     CDS             complement(5104..5601)
                     /gene="rrrD"
                     /locus_tag="POA77_004566"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDI6367894.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(5601..5816)
                     /gene="essD"
                     /locus_tag="POA77_004567"
     CDS             complement(5601..5816)
                     /gene="essD"
                     /locus_tag="POA77_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDI6367895.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1600 a         1606 c         1360 g         1587 t
ORIGIN      
        1 tttacctgtc atcaggtcat gcagtgccgc acgggcagcg gcaagttctt cctgtcgcgt
       61 cattcatcct ctccggataa ggcacgggcg taatctgcca gtgttttctt gttggttgct
      121 gcaccatcct cttcctgcag gctcgccagc agcgcactga gatccagctg ccagcgggaa
      181 atactgatgc gcagcgccgc cagcgcataa acgaagcagt cgagtgcctc attgcgtcgc
      241 tttttgctgt cccacagtat ttttttcctg ccatccaccc atttttcgac ctgctcttca
      301 gcagtcagct gctgcgcttc ggtcagatca aaaatatccg ggttattcgg gaagtgaacg
      361 gcaccgggaa gcggttcatc cccttccggc gtcagtgtga agcggttata aatctgctct
      421 ttcgcggtat ccgtaccgat ttcggtaagg taaaccccgt ttttgtttcg cttacgtggc
      481 atgctggcca caggcttacc gtagacggat gcccctttaa tggggatcac ccggaacagc
      541 ccatgttttt tcgagcgttc atacacaatg gtcggatcaa tcccgccagt atcccagcag
      601 atacgggata ccgacatttc tgcaccattc cggcgggtat aggttttatt gatggcctca
      661 tccacacgca gcagcgtctg ttcatcgtcg tggcggccca taataatctg ccggtcaatc
      721 agccagcttt cctcacccgg cccccatccc catacgcgca tttcgtagcg atccagctgg
      781 gagtcgatac cggcggtcag gtaagccaca cggtcaggaa cgggcgctga ataatgctct
      841 ttccgctctg ccatcacttc agcatccgga cgttcgccaa ttttcgcctc ccacgtctca
      901 ccgagcgtgg tgtttacgaa ggttttacgt tttcccgtat cccctttcgt tttcatccag
      961 tctttgacaa tctgcaccca ggtggtgaac gggctgtacg ccgtccagat gtgaaaggtc
     1021 acgctgtccg gcggctcaat ctcttcaccg gatgacgaaa accagagaat gccatcacgg
     1081 gtccagatcc cggtcttttc gcagatataa cgggcatcag tgaagtccag ttcctgctgg
     1141 cggatgacgc aggcattatg ttcgcagaga taaaacacgc tggagggatc atccggcgtc
     1201 catttgaggc taaacgacgt ctctttatcg ccaaatttaa ggtactgctc ctccccgcag
     1261 tgcggacagg caacatgaaa acgcataaaa tgcggggatt cactggctgc acgctcaatc
     1321 tggcaggtgc ctctcacttt gggggtggag ccacggatgg actttggcca gaccgagcct
     1381 tcaatacttt tgtcgccaag gaacgtcgga gagccttcct gttcaatatc ctcatcaaag
     1441 gcagcaagtt catcataacc cgccacatcc accgactttt cacggtagtt ttttgccgct
     1501 ttaccgccca ggcaccagaa gccacgacca ttggaaaaac gcttcatggt gagcgtgtta
     1561 tcccggtgct ttttgccata ccacggagcc agcgccagca gcaacggaat atcgcggatg
     1621 gtcggctcaa cgtgggtttt cataaagttc tcggcatcac catccgtcgg caaccagata
     1681 agtgtgttgc gctgcttatg ctctatgaag taggcataaa cacccaacag cattttggaa
     1741 taaccaacac gggcagactt caccacattc acctcgcgga tgtagtcgct gcccatcgca
     1801 ttcatgatgg cccgctgaaa gggcagtgtt tcccagcgcc cttcctggta tgcggattct
     1861 ttcgggagat agtaattagc atccgcccat tcaacggcgg tctgtggctc cggcctgaac
     1921 agtgagcgaa gcccggcgcg gacaaaatgc cgcagcctgt taacctgact gttcgatata
     1981 ttcactcagc aaccccggta tcagttcatc cagcgcggct gctttgttca tggctttgat
     2041 gatatcccgt ttcaggaaat caacatgtcg gttttccagt tccggaaaac gccgctgcac
     2101 cgacaggggg atcccgtcga gaatactggc aatttcacct gcgatccgcg acaacacgaa
     2161 agtacagaat gcggtttcca ccacttcagc ggagtctctg gcattcttca gttcctgtgc
     2221 gtcggcctgc gcacgcgtaa gtcgatggcg ttcgtactca atagtccctg gctggagatc
     2281 tgcctcgctg gcctgccgca gttcttcaac ctcccggcgc agcttttcgt tctcaatttc
     2341 agcatccctt tcggcatacc attttatgac ggcggcagag tcataaagca cctcattacc
     2401 cttgccaccg cctcgcagaa cgggcattcc ctgttcctgc cagttctgaa tggtacggat
     2461 actcgcaccg aaaatgtcag ccagctgctt tttgttgact tccattgttc attccacgga
     2521 caaaaacaga gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgcttc
     2581 ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa
     2641 cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac
     2701 ctgtcggatc gccggaaagg acccgcaaaa tgataataat tatcatctgc atgtcacaac
     2761 gtgcatctac gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcatattaat
     2821 tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactaaat
     2881 acgggacaac ctcatgtcaa cgaagaacag aacccgcaga acaacaatcc gcaacatccg
     2941 ctttcctaac caaatgattg aacaaattaa catcgctctt gatcaaaaag ggtccgggaa
     3001 tttctcagcc tgggtcattg aagcctgccg ccggagactg tgctcagaaa aaagagtttc
     3061 tcctgaagca aacaaagaaa agagtgacat tactgaattg ctcagaaaac agatcagacc
     3121 agattgaagc aatttagata atcgtgcaga ctacgcccct catatcacat ggaaggtact
     3181 acaatggctc aggttgccat ttttaaacaa atattcgata aagtgcgaaa taatttaaac
     3241 tatcactggt tttattctga actaaaacgt cacaatgtct cacattacat ttactattta
     3301 gctacagaga atattcatct tgttcttgaa aacgataata cggttttaat aaaaggacag
     3361 ggtaaggttg taaatgtaag attttcaaaa aataaatgcc ttatagaagc caccttaaaa
     3421 ggattcaaat caggagagtt atcattttac gaatacagga aaaatcttgc tacagcaggg
     3481 gttttcagat ggattacaaa tatccacgaa aacaaaaggt attactatac ctttgataat
     3541 tcattactct ttactgagaa cattcagaac actacacaaa tatttccgca ctaaatcata
     3601 acgtccggtt tcttccgtgc cagaaccgga ctcgctggca tgatgaaata tgtgtacccg
     3661 gtaaccccgg tgtgcatcgt ttttgattat tcccgcacac tcgcgcagaa ggagttcccc
     3721 gtcgggctac ggtctctgtt aatacgggaa tacggcgacg atacagcgca tgatgtgtca
     3781 ggcttgaatg cctttatccg ttaaaaggga tatcagttaa gttatcccgt gcagggtata
     3841 agccattatc aagcccaccc gtagatgggc tttgtaatgg atagccgttg ctcagttcta
     3901 gtaatgcttt gattttttcg ataacgcagt tttgcgtttg ccatcagcac gcgatatcga
     3961 gagtcaactg cagttgctcg cgccagtact caacatttgc ttcaataacc ggcttatccc
     4021 atcgccagcg agccatctct cttgccccat tgctggcttt tgatttccgg tcatcgcgaa
     4081 tgcgacatgc ttgctcatat ttctgctgct cagtcagttc accgcgaagc agactatcaa
     4141 tgtgcaggtc gcaccacaca gcaaaacgag catcacacca acgggcaaat gcaactgaaa
     4201 gttttggatg tagccacgta ccaccacccc tgtcctttcg tgccttgctg gtttttacat
     4261 acctcgattg tgagggatgt aaaatttgag attctttccc ggtcaacgct tcgtctaaag
     4321 cacgaacgta ttcaagcgtt tctgccaaac gcatccagtt atcaatgcgt ttcccaaatc
     4381 tctcagcaac acctgtgacg ttgatccaac catcagtgtt gaaactgaca atttcacctt
     4441 tgtaattaag tggcacgata ttcataacgt ttacctacca tttgaaatga acctttgccg
     4501 cacaggaaac cagcccaccg aggctcgcca gcactaactg gtatcctcaa aggcccattc
     4561 caaaggggca ggttcggtgt aaaaaaaaca tgcgttgcgg tacgcattta ttgcaaaaaa
     4621 gccccgcatc gcgaggctca ttaaatggac tttgtgattt gcaaaaaaat tatttcagac
     4681 attgcgtcct gatgtactcc tgcaggtagt taacctgcgc ggttatcctg tcgattccac
     4741 ttcggagacg gtaataattg agttcagcat ctgctgtaag tcttgggctt tctccatcgc
     4801 ccatgctgct ggctccggtc tttgactttg cacaggaggc ggcgacttgc agccgcttac
     4861 gcccagcaga aacatcatca cggagacttt cgatagtcgc gttagcatca gcaagctcct
     4921 ttgtatatct ggcatcgagt tctgctacgt cacgttgacg cttctgcatg tcagcgatga
     4981 tggatgtggc tttgtcgcgc tgttctttgt aggtcatggc gttatcacgg taatgattaa
     5041 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggttactc
     5101 tgttcattgc tgaccccaca aacagatttc acgctcaatc tcacgacgag tcatgagacc
     5161 tttccattgc ttaccgccag catatgtcca gcgacgtagc tgatcacatg cgcctttgat
     5221 atcgccctgg tttattttgc gaagaagcgt cgatgttctg aaattgccag cacccacgtt
     5281 gtaaacgaat gagtaaagag cgccgcgcat tgtttccggt atatcgactt tgatgtacgg
     5341 gttaatttgt ctggcgaccg tggcaaggtc tttattcagg agggctttgc attctgcttt
     5401 ggtatacgtt ttaccgagca tgatgtcttt tcctgtatgc ccgtgacata cagtccatac
     5461 accaacaata tctttgtatg gtatgtagct gacaccttcc agaccatcgt taccacttgg
     5521 gccagtaatt aacactgatg ctatagcaat tgctccgcca ccaatagcag cagcaacggc
     5581 ttttcgtaat gatggaggca ttattcacct ctcgcagcct tgcgcttatc ttctttaatc
     5641 ttgaaataaa ggtttgtcag gtacgtcagc aggccaaata ccaggctacc cagcacacct
     5701 attgctgccc actgtgaagg cgtgacttta tcgagcagct gtaaaaacca gtaaccggca
     5761 ctacctgctg aggtgccata ggcgacaccc gttgttaact tatccatgga tttcataacc
     5821 ccacctcgca gacaaagcgg gtgtaaattg agggaataca acgtatcgca aaaaagcaga
     5881 aacgtaacag actcggagtc agtgaataac tcaggtattg agttatcagc taatatcgag
     5941 actcaaaaaa tggaaaaacc agctcgacgg cgggtttaag ctgtgtgacg aagtaaccac
     6001 tcttaacagc ataaccaatt ttttacgtac gtaaaccact gaatgatatt tatgagaatg
     6061 ctaccgagtg ttcaaaacac caccacaaat acataagaaa acctcaacaa ataaccaata
     6121 aataatttca gaggttattt ttagttgatt taa
//
LOCUS       DAMZII010000213         6145 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZII010000213 DAMZII010000000
VERSION     DAMZII010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.215"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            47..823
                     /gene="exuR"
                     /locus_tag="POA77_004568"
     CDS             47..823
                     /gene="exuR"
                     /locus_tag="POA77_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDI6367896.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1168..1830
                     /locus_tag="POA77_004569"
     CDS             1168..1830
                     /locus_tag="POA77_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6367897.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1834..2217
                     /gene="mzrA"
                     /locus_tag="POA77_004570"
     CDS             1834..2217
                     /gene="mzrA"
                     /locus_tag="POA77_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDI6367898.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2364..2732
                     /locus_tag="POA77_004571"
     CDS             2364..2732
                     /locus_tag="POA77_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDI6367899.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2770..3075
                     /locus_tag="POA77_004572"
     CDS             2770..3075
                     /locus_tag="POA77_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI6367900.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3078..3482
                     /locus_tag="POA77_004573"
     CDS             3078..3482
                     /locus_tag="POA77_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI6367901.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3472..3771
                     /locus_tag="POA77_004574"
     CDS             3472..3771
                     /locus_tag="POA77_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDI6367902.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3957..4349
                     /locus_tag="POA77_004575"
     CDS             3957..4349
                     /locus_tag="POA77_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6367903.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4419..5405
                     /gene="yqjG"
                     /locus_tag="POA77_004576"
     CDS             4419..5405
                     /gene="yqjG"
                     /locus_tag="POA77_004576"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDI6367904.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5698..6063
                     /locus_tag="POA77_004577"
     CDS             5698..6063
                     /locus_tag="POA77_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6367905.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1535 a         1512 c         1550 g         1548 t
ORIGIN      
        1 aaagtggtat gataacttcg tccgcattcc actccgggag acgcatatgg aaatcactga
       61 accacgccgt ttgtatcaac aacttgccgc tgacctgaaa gagcgcatcg aacagggcgt
      121 ctatctggta ggtgataaac tgcctgcaga acgctttatt gccgatgaaa agaacgtcag
      181 ccgtacggta gttcgtgaag ccatcatcat gctggaagtt gaaggctatg tcgaagtgcg
      241 taaaggttca ggtattcatg tggtttccaa ccagccgcgc catcagcagg cagctgacaa
      301 taatatggaa ttcgccaatt acggtccgtt tgagttgctt caggctcgcc agctcatcga
      361 aagtaatatt gccgaattcg cggcaactca ggtaacgaaa caggacatca tgaaactgat
      421 ggccattcag gaacaggcgc gcggcgaaca atgcttccgt gattccgagt gggatttaca
      481 gttccacatt caggtagccc tggcgacgca gaactccgcc ctggcggcca tcgttgaaaa
      541 aatgtggaca caacgtagcc ataacccgta ctggaaaaaa ctgcacgaac acattgattc
      601 ccgtaccgtc gataactggt gtgatgacca cgatcaaatc ctcaaggcgc tgattcgcaa
      661 agatcctcat gctgctaagc tggcaatgtg gcagcacctg gaaaacacca agatcatgtt
      721 attcaacgaa accagcgacg acttcgagtt caatgccgac cgctatctat tcgccgaaaa
      781 cccggtggtg catctcgata cggccaccag tggcagtaaa tgaaatacct gtccgacagg
      841 cgctgcgacg cgcctgtttg tctcgtgggt aagcgaaaag tataaagtgt cagcctgtgt
      901 aaatcctctc gccaccctcc cctgcattca gcaaaatcag actccacgga catgtaattt
      961 tgataacgaa caacgttgac ctttgttaca attagattca atttgaattt atgtttttga
     1021 atgctttctt atctcacgat ttaacaggga atagttcagg ctgtattgat gtatcaaacc
     1081 cgcagaacat accaaaacag caataacatt gcggtagtgc atcttttaaa accagcgtgg
     1141 cgttaaccga ttcaccagga ataatgaatg gaacttttga cccaattgct gcaagccctg
     1201 tgggcgcagg attttgaaac cctggccaat ccatcgatga ttggcatgtt gtattttgtc
     1261 ttgtttgtaa ttttgttcct tgaaaacggc ttgcttccag cggccttttt accgggcgac
     1321 agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg cgatgggtta cccgcaaacg
     1381 attctgctgc tgaccgttgc cgccagcctc ggctgctggg tcagctatat tcaggggcga
     1441 tggctgggca atacccgcac cgtacaaaac tggctatctc atttacccgc gcattatcat
     1501 caacgcgcac accatctttt tcataaacac ggtttatcgg cgctgttaat tggtcgcttt
     1561 attgcgtttg tcagaacact gctgccgacg attgccgggt tatcagggct gaataacgcg
     1621 cgctttcagt ttttcaactg gatgagcggt ctgctgtggg tattgatcct gacaactctg
     1681 ggttacatgc tcggcaaaac gccggtattt ttaaagtacg aggaccagct gatgtcatgc
     1741 ctgatgctgc tcccggtggt gctgctggtg tttggcctgg caggttctct ggtcgtgtta
     1801 tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa tacctcgcat gtcgcttcgc
     1861 cagctagcct ggtccggcac tgttttactt ctggtcggca cgctgttgct ggcctggtcc
     1921 gcggttcgcc agcaagagtc tacgctggcg attcgtgccg ttcatcaagg cacaacgatg
     1981 ccagacggtt tttcgatctg gcatcacctt gacgctcatg gcattccttt caaaagtatc
     2041 acccccaaaa acgacactct gttaattaca tttgactcca gcgaccagag cgccgccgca
     2101 aaagcggtcc tcgacagaac attgccccat ggctacatca ttgcgcagca ggacaataac
     2161 agtcaggcta tgcagtggct gacccggtta cgggataact ctcatcgctt cggataactt
     2221 cctgttatcc gaaacatatc actcactttg gtgatttcac cgtaactgtc tatgattaat
     2281 gaggcggtgg ttaacactac cgcttcgttg tctggacaat cgttcctttg taataggtcc
     2341 gaatcacaat ggaaggttca agaatgaaat accgcatcgc tttagctgtt tctctctttg
     2401 ctcttagtgc cggtagttat gccactaccc tgtgtcagga aaaggagcaa aatatcctta
     2461 aggagatcag ctatgccgaa aaacaccaaa accagaatcg tattgacggt ctgaataaag
     2521 ccctgagtga agtccgggcc aactgttcag atagccagct gcgtgccgat catcacaaga
     2581 aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca agatttagcc gaggcgaagc
     2641 aaaaaggcga tgccgataag attgccaaac gcgaacggaa actggcagaa gcgcaggaag
     2701 agctgaaaaa gctggaagcg cgcgactact aactcacaat agtcactact tactcacctg
     2761 gagaaaacta tgtcgaaaga acacactacg gaacatctgc gtgctgagtt gaaatccctt
     2821 tccgatacgc tggaagaggt gcttagctca tctggcgaga agtcgaaaga agagttgagt
     2881 aagattcgta gcaaagcgga gcaggcactg aaacagagcc gttatcgcct gggtgaaacc
     2941 ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt
     3001 gaaaatccgt ggaccggcgt gggcattggc gctgcaatcg gtgtagtgct cggcgttctg
     3061 ctgtcgcgtc gttaattatg gcggacactc atcacgcgca agggcccggt aaaagcgttc
     3121 tgggcatcgg gcagcgaatt gtttccatca tggttgaaat ggtggaaaca cgtctgcggc
     3181 tggcggtggt ggagctggaa gaggaaaaag ctaatctctt tcaactttta ctgatgctgg
     3241 gcctgacgat gcttttcgct gcatttggtc ttatgagcct gatggtgcta attatttggg
     3301 cggttgatcc gcaatatcgc ctgaatgcga tgattgccac caccgtggtg ttgctgctac
     3361 tggcactgat tggcggtatc tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc
     3421 atacacgcca tgagctggca aacgatcggc aactgctcga ggaggagtcc cgtgagcagt
     3481 aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc agatccagca acaacggctg
     3541 gatctttccg ccagtcgtcg tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg
     3601 aatatgctgc taagtctgcg ctcctgggcg ctggttggca gtagcgtgat ggcgatctgg
     3661 acgattcgcc atcctaatat gctggtccgc tgggccagac gcggttttgg cgtatggagc
     3721 gcctggcgtc tggttaaaac gaccctcaag cagcaacagc ttcgcggtta acttccctct
     3781 ggccggagca attccggcct tatccctcaa attttttgaa gatttttgac agttttcctt
     3841 gctaacaatc atcattcacc acgtttatga ttctctccat cgacagcaac gacgctaata
     3901 ccgcgccctt gcacaaaaaa acaatcagca gcctgagtgg cgcagtggag agtatgatga
     3961 aaaaattaga agatgttggt gtactggtag cgcgcatttt aatgccgatt ctgtttatta
     4021 ccgcaggctg gggaaaaatc accgcttact ctggtaccca acaatatatg gaagcaatgg
     4081 gtgtcccggg ttttatgctg ccgctggtga ttctgcttga gttcggtggt ggtctggcaa
     4141 tcctgttcgg tttcctgact cgcaccacag ccctgtttac tgcgggcttt acgctgctga
     4201 cggcattttt atttcacagc aactttgctg aaggcgtcaa ctcgctgatg ttcatgaaaa
     4261 acctgacaat ttctggcgga ttcctgctgc tggcaattac cggtccgggc gcgtatagca
     4321 tcgaccgcct gctgaataaa aagtggtaag cacgcgctat acttaacgat aaaaaacgag
     4381 gaggaagctc ctcgtttttg ctattggagg agagaaaaat gggtcaactg attgacggcg
     4441 tctggcatga cacctggtac gataccaaat ctaccggcgg taaatttcaa cgttcagctt
     4501 ccgcatttcg taactggctc actgccgatg gcgcacctgg ccccactggc acaggcggtt
     4561 ttatcgcaga gaaagatcgt tatcatctct atatttcact cgcctgcccg tgggcgcacc
     4621 gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga
     4681 acccgctgat gctggaaaac ggctggacct ttgatgacag ttttccggga gcaaccggag
     4741 atacgctcta tcaacatgaa tttctgtatc agctttatct ccacgccgat ccacactaca
     4801 gcggacgagt tactgttccc gtgctgtggg acaaaaagaa ccacaccatc gtcagcaacg
     4861 aatcagcaga aatcatccgc atgttcaata ccgcgtttga tgcgctgggc gcaaaagcgg
     4921 gtgattacta cccaccagcc ctgcaaacga aaattgacga acttaacggc tggatttatg
     4981 acaccgttaa caacggcgtg tataaagctg gttttgccac cagccagcaa gcctacgacg
     5041 aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga gcagatttta ggccagcatc
     5101 gttacctgac cggcaaccag ctaaccgaag ccgatattcg cctgtggact acgctggtgc
     5161 gttttgatcc agtgtatgtg acccacttca agtgtgataa gcaccgcatc agcgattacc
     5221 tgaatctgta tggcttcctg cgcgatatct accagatgcc ggggatcgcc gaaactgtca
     5281 atttcgatca catccgtaat cattacttcc gcagccataa gaccatcaac cctacgggga
     5341 ttatttcaat tggtccgtgg caggatctcg atgaaccgca tggacgagat gttcgcttcg
     5401 gttaaaaaaa ggcaccttac gaggtgcctc tgataattag ctaagtgcat gttgttcatg
     5461 tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacat ttatattcaa tatgttgcaa
     5521 tgaccgcgca ggccagataa gcgtagcgca tctggcgatt ttgcgcttgt catcagtctt
     5581 agcgcgcctt cacagcactt tattaattcc ttgaacgaat atttactgcc atttcattca
     5641 cgtctattct taatttgctg ctttaaaaac aagtgattga gcaaaattga ggcaaaaatg
     5701 gactggtatc tgaaagtact aaaaaattat gtcggtttcc ggggtcgtgc gcggcgcaaa
     5761 gagtactgga tgtttattct ggtcaacatc atctttacgt tcgtgctggg gctgctggat
     5821 aaaatgttag gctggcaacg cgctggtggc gaaggcatcc tgacgacaat ctacggtatt
     5881 ctggtgtttt taccgtggtg ggcagttcag ttccgccgcc tgcacgacac cgaccgctcg
     5941 gcgtggtggg cactgttgtt cttaatcccg tttatcggct ggcttattat catcgtcttt
     6001 aactgccagg cgggtacgcc aggcgaaaac cgctttggac cagatccgaa actggaacct
     6061 taaaacaaat ctgctcgccg gatgtctgac tcatccggct tttatcatca tgttgatgaa
     6121 tgaataattt actaatgttt attta
//
LOCUS       DAMZII010000214         6123 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZII010000214 DAMZII010000000
VERSION     DAMZII010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.216"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..718)
                     /locus_tag="POA77_004578"
     CDS             complement(<1..718)
                     /locus_tag="POA77_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HDI6367906.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVG"
     gene            complement(1173..2401)
                     /locus_tag="POA77_004579"
     CDS             complement(join(1173..2086,2086..2401))
                     /locus_tag="POA77_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDI6367907.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCSDREALHWAVTTGGFN
                     SETVQDVMPGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     TESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            3108..3401
                     /gene="iss"
                     /locus_tag="POA77_004580"
     CDS             3108..3401
                     /gene="iss"
                     /locus_tag="POA77_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDI6367908.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(3459..3600)
                     /locus_tag="POA77_004581"
                     /pseudo
     CDS             complement(3459..3600)
                     /locus_tag="POA77_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            4211..5310
                     /locus_tag="POA77_004582"
                     /pseudo
     CDS             4211..5310
                     /locus_tag="POA77_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1594 a         1449 c         1419 g         1661 t
ORIGIN      
        1 ttcctacaag tgcttccagt tcactgatga gtaactcaac acggtcaaaa ttgtagtccg
       61 ggatcgccag taatgtgctc agttcaatat ttccccatga taccgcgagc acatccacgg
      121 aggggttaag tgggtgtatg gcttttgcca cagcggagat aatgtcaaag ggcaggcggt
      181 catttctggt cagcagcagc ctgttgcaga acatgatttg ctcggccagc aaattctcca
      241 ggccgcgttt gttatgccgg agattttcct gaagagtggg gatcagtttt ttcccatcat
      301 tgtaatcttc atggagcatg acagtatcaa cgagtgttaa aaacgccttc aggcgtacac
      361 gtgaatggcg acgcaggtat ttgaccagag gaagcggatg actgcttccg gaggtttcaa
      421 tcagcatcac atccggcttg ttgtgatcaa gcaggtggtt aattgcgaga tccatctttt
      481 tgaggcctgc ggggctgctg atgctgtctg cggtgatact tacgaaatta ttgtcttttg
      541 catctacaat atctgtgttg gcgatcagca cgccgtccac atccagttcg ctcatgtcat
      601 tgactatgac agagactgaa agctgttttt cgtgtgcctg tatgagcagg tttttcagca
      661 gagtggtttt ccctgcgccg aggaagccat tgagaatgat gacagatgtt gtttccatat
      721 cagcagtaca ccagttagtt agcgaggaag gcgcttggtg gtacaaagag catccggatt
      781 gaatcgcata acgtcgacgg gagttgttgt cgtttttcct gtgaggtctc aacctgccac
      841 agaacgcacc ggatttattg gtatttagtc tccatcagca taattatcac catttttatc
      901 tccgggggaa gatttgttct gaacaggagt gggaggtgtg acctcctgaa agtaattaca
      961 gattgctagt tgttgtattt ttattcgaat atgtctcttc gtatcagtga tagcacctag
     1021 ataaagtata ttccgggagc gttatctgtt ctagatatca gagagtcagt catttgtaaa
     1081 aaagagagct gcatcattct atttttcctg tctattcacc ctgttgtcgt atctaaaaga
     1141 catccgttcc gaggatgtcg gtggatttgc ccctatattt ccagacatct gttatcactt
     1201 aacccattac aagcccgctg ccgcagatat tcccgtggcg agcgataacc cagcgcacta
     1261 tgcggatgcc attcgttata atgctcgaac gcctctgcaa ggttctttgc tgccgttaac
     1321 ccgtctggtt tgggcatgat actgatgtag tcacgcttta tcgttttcac gaagctctct
     1381 gcgattccgt tactctccgt actccgcacc gccgtgttct tcggttcaag tcccaacatc
     1441 cgggcgaact ggcgtgtttc attagcccgg tagcatgaac cattatccgt cagccactcc
     1501 actggagacg acggaagatc gttgccgaag cggcgttcca ccgctcctgg catgacgtcc
     1561 tgtactgttt cactgttgaa gccgccggta gtgaccgccc agtgcagtgc ctcacgatca
     1621 ctgcagtcca gcgcgaacgt gacacgcagt ctctctccgt tatcacagca gaactcgaac
     1681 ccgtcagagc accatcgctg attgctttct ttcacggcca ctctgcctgt atgtgcccgt
     1741 ttcgatggcg gtacagcagg ttttcgctca agcaacagcg cattctggcg catgatccgg
     1801 taaacacgtt tggcattgat cgcaggcata ccatcaagtt ctgcctgtct gcgaagcagc
     1861 gcccataccc gacgataacc atacgtgggc agctctccga taacatggtg tatacggaga
     1921 agcacatccg tatcatcagt gtgacgactg cggcggccat ccatccagtc atcggttcgt
     1981 ctgagaatga cgtgcaactg cgcacgcgac acccggagac aacggctgac taagcttact
     2041 ccccatcccc gggcaataag ggcgcgtgcg ctatccactt ttttgcccgt ccatattcaa
     2101 cggcttcttt gaggagttca ttttccatcg ttttcttgcc gagcaggcgc tggagttctt
     2161 taatctgctt catggcggca gcaagttcag aggcaggaac aacctgttct ccggcggcga
     2221 cagcagtaag acttccttcc tggtattgct tacgccagag aaataactgg ctggctgcta
     2281 caccatgttg ccgggcaacg agggagaccg tcatccccgg ttcaaagctc tgctgaacaa
     2341 ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc cggtcctaag acatcaatca
     2401 tctgctctcc aatgactagt ctaaaaacta gtattaagac tatcacttaa ataagtgata
     2461 ctggttgtct ggagattcag ggggcctgtc tattctcctt cacggtaata cgcagttata
     2521 gttgcagagc aatgtaatgg atcttttata ttaagaaatt gtgtgagtgg agaggtgcac
     2581 gaatcctaga ggcagaatgt ttctccacat gcttgtggag aaaccgccgc attttgcccg
     2641 ttctgtacga tactccgtag ccagagataa gatattctgt cgctgtatgc tacatacatt
     2701 acactatacg aaatcaggtc tgttcaatca gtaaggaaat aaacggacaa acgttatgta
     2761 acccagacag agatatattg tctgggaaaa tagtggcttt tgtggcgaaa actagtaaaa
     2821 cagcaacccg aaccacttga tgtgcatcgt ttttgattat tcccgtatac tcttgcagaa
     2881 ggagttctcc gtcgggctac tgtcatggtt aatgcgggga atatggcgac aatacaacac
     2941 acctaaaaga gtaatggaca gatgaagcgg tttattcatt tcccatgatt ctgagtacct
     3001 accaagtctg agtaaccact tttatacttt taattttcgt tcatttagct atcgtttaat
     3061 tattatcaca taggattctg ccgtttttaa caatgcagga taataagatg aaaaaaatgt
     3121 tattttctgc cgctctggca atgcttatta caggatgtgc tcaacaaacg tttactgttg
     3181 gaaacaaacc gacagcagta acaccaaagg aaaccatcac tcatcatttc ttcgtttcgg
     3241 gaattggaca agagaaaact gttgatgcag ccaaaatttg tggcggtgca gaaaatgttg
     3301 ttaaaacaga aactcagcaa acattcgtaa atggattgct cggttttatc acttttggca
     3361 tctatactcc gctggaagcc cgggtatatt gctcacaata gttgcccatc gatatgggga
     3421 gctcatctgc actgttcatt aatatacttc tgggctccct acagttgttt ttgcatagtg
     3481 ataagcctct ctctgaggga ggaaataatc ctgttcagcg atgtctgcca gtcggggggc
     3541 tgcattatcc acgcccgagg cggtggtggc ttcacgcggg gatgggcaga ttgatctgat
     3601 atgcaaccga cgacgaccag cggcaacatc atcacgcaga gcttcatttt cagatttggg
     3661 ccaccttttg atttctcgtt taacttttgc agtcaccaga ctgtgattta ttttaacaaa
     3721 tcaatagctt tcataaaatt ctcgaagctg aaagcttaac tgaaccaacc agccaggctg
     3781 gtgttttcta aagacaaaaa ggccatcagc aaaacactga tagtctgaat cacctttatc
     3841 aatcatgtat gaagaaaata tgcgcaatta gttgacagtg attatcattt tcattaaaaa
     3901 gagcgcgtag cattcttatt tcatgaggaa atttacccgc caacaacctg agtagcgaaa
     3961 gctgttcatc cccaatattt ttgctggcgg gttctttttt ctactgtcct cctccccaga
     4021 ggaagcattc ccattaatca ttactggcga ggaggcaaga gaaaaagtcg gttctttgtc
     4081 cctaaataac agcatcattt cagtccatgc aaaatctgat ttaccagctc acataataac
     4141 ctgatgatac acagtgtaag ttcacagaga tattgcaatt gcctccggat aagtaagggg
     4201 agattgcact atgcaaatgc agcacctgag gttggctatc ctaagcagcc gattatgcgt
     4261 tgaggctttc agtgatggct gatatagcaa caatgagaat gaaggctctg cacttctggg
     4321 ataaacacgg tatttctgca gcttctgaag cctttggcgt atcctgccgc acgctttact
     4381 ggtggcgtca gttactgaac aagggaggac ctgaggggct aatcccgcac agtaaggcac
     4441 ctctggtgcg acgaaaaaag cactggcatc ccgatgtgct gaaagagatt cgacgcctca
     4501 ggacagagct gccgaacctc ggtaaagagc agatttttgt tcgcctgaag ccctggtgcg
     4561 cataacgcca tcagacctgc ccgagtgttt ccaccatcgg cagaatgatc gccaccgcac
     4621 acgataaaat gcggatgata ccagtacgtc tgagctcgcg gggtaaggct ctgcttgtca
     4681 aaaagcgaac caccaacccc ccgcaggcca aagcactacc gtccggtaaa gacaggagag
     4741 ctcattggga tggacgccat tgagctcaga atgggtgaaa tgcgtcgcta tatcattacc
     4801 atgatcgacg aacacagcga ttatgtactg gcgctggctg tgccgtcgct caacagtgat
     4861 atcgtcaatc actttttcag ccgtgcagcc cggctgttcc ctgtcggtat cagccagatc
     4921 atcacagata acgggaaaga gttcctggga agctttgaca aaacgctgca ggaagccgcc
     4981 atcaaacacc tctggaccta tccctacacg ccaaaaatga acgctatctg tgaacgtttt
     5041 aaccgggcat taagagagca attcattgaa tttaatgaaa ttttactctt tgaggatctg
     5101 gcgttgttta acctgaaact ggcggaatat ctggcgctgt ataacagcaa aagactccac
     5161 aaggcgctcg cactaacgac gcccgtggaa tatattttaa aagagaacaa aaattgcaat
     5221 atgtggtgga cccatacaca gcaaacgcac aggccgcccc ggttgggtag atatgtggga
     5281 tggctggttc agacttcagg atatggttga ggctggtgat gaagtctctt tatcaggaga
     5341 tctgatcaag agacagccgc gcagggggat tcactatgct ggattctttt aacaaattct
     5401 ccgctaacca aaaaatagac tttttgcatt cgtaaatgcg ttaagagata atttttaaag
     5461 ttccatcaat ctcccggcca gttcgtctcg cggcattact cactatccgc acaacttcag
     5521 taagatcatg actttttcaa tcgttatctg ttgaccaagc tttgaaacca ctagcgtccc
     5581 gtcaacctga cctggaatcg tcgagaaccg cccatagttg tgaattggct agagtgtgat
     5641 ggaaagcaac gctaaacgac ctagtcacac attctctttt ccctaacaat gaccatttag
     5701 tgaaataacg tatccaaaat gatgacgtga aagcctgaca ctggcagcga aaagccgaaa
     5761 aaactcacct ggtcaggagc aaaaatcgga ttattcgcca acagcgtaat atcagcagta
     5821 gttctcgaca cgcttgcgct atagacagag gatgaaacgc tgctggtcgg agttctctat
     5881 acatttttgg cgacggcaga accgctcacc aatgccttaa cgtctatcag ccttccggtg
     5941 agttggaaca gcatgctcag cgtttgcaag atattctcca cggtctgtag cagcgtaggt
     6001 ttcaatttaa atatccccag atagccctct gggagcttcc ctttttatta agttttaatt
     6061 accgttgcaa tagataaatg atattggtaa ttattatcat tagcatttgt gtctgtgttg
     6121 ttt
//
LOCUS       DAMZII010000215         6029 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZII010000215 DAMZII010000000
VERSION     DAMZII010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.217"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..628)
                     /locus_tag="POA77_004583"
     CDS             complement(<1..628)
                     /locus_tag="POA77_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6367909.1"
                     /translation="MTHQTHTIAESNNFIVLDKFTKVMDTGDSYQSELDLERELIQDL
                     LNQGYELISVKSPTAMLANVRDQLQRLNGVEFNESEWRRFAEQYLDSPSDSSLDKTRK
                     IHIDYICDFTFDDGRLENIYLIDKKNLLRNKVQVIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSDNSLFKYLQLFVISNGTDT"
     gene            complement(749..2008)
                     /locus_tag="POA77_004584"
     CDS             complement(749..2008)
                     /locus_tag="POA77_004584"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6367910.1"
                     /translation="MSGLSYLEKLLDGAEVEWRTLDSVGQFLRGKRFVKSDLISEGVP
                     CIHYGEMYTHYGISAEETKSFLDEGVAEKLRVANHGDVIIVAAGETIEDIGNGTAWLG
                     KNDVVFHDACFSFKSELNSKYVSYFLRTNLFKEQIKRGVSSGKISSINAKGLGMAEIP
                     IPCPNNPEKSLAIQSEIVRILDKFTALTAELTAELTMRKKQYNYYRDLLLSFEEGEVE
                     WKTLEEVLVDKFWIMPATPKFDDNGEIPYVTSKNISGGNIDFERVKHISRDDFLSISK
                     NRPILKGDFLISMIGTIGEIARVKCLDPDFYGQNMYLIRLNEELLHPRYFLHFFDSPR
                     MKFYFKSVKNNSGQGYLKANNIDGLSIPLPSIDEQQKIAFILDKFDTLTNPINEGLPR
                     EIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            complement(2005..3561)
                     /locus_tag="POA77_004585"
     CDS             complement(2005..3561)
                     /locus_tag="POA77_004585"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6367911.1"
                     /translation="MTSLQQRAELHRQIWAIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYMEAGDESIHYAALDDSIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNSRLAAVLKGVE
                     GLNLGNFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKHFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IRLGNTLTEPHFGDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTNVQFIDASELFKKETNNNILTDDHIRQIMSVFDSKADTDHL
                     AKTVPYETVASNDYNLSVSSYVAAKDTREIVNITELNAELKTTVSKIDQLRQDIDAIV
                     AEIEGSEVQA"
     gene            complement(3765..5630)
                     /locus_tag="POA77_004586"
     CDS             complement(3765..5630)
                     /locus_tag="POA77_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI6367912.1"
                     /translation="MAGQYEKAITIKQAIDSINLRHYLLPAIQRKFVWSYRQICLLFD
                     SIMRDYPINSFMMWDIRSASIKNDYKFYEFLKEYCQRFNEENPYVPTNAGFHDFKAVI
                     DGQQRLTSLYIGLCGTYAYKKPREWWPSAQDDRILPPRKLYVDLTAPLDSDDEPMMKY
                     NFRFLTDKEYTDSLTDNKHHWFCLHEIFKYEQIDSPDDILFKVVVPELEKRGLISSEF
                     SRKTLLKLYTKIRTENLIHYFNESSQDIDHVLDVFIRTNSGGTKLEFSDLLMSIAVAH
                     WQGDFRRELDELTKNIHQNNEMGFYIERDWFLKTSLMLIDSDVRFKVKNFTSEEVGKI
                     QQQWSEIKSCIKETFVLIRRFGINPQSLISKNAVIPVVYWLYKKQTNGHPLYTTINLL
                     NKNHNERSVISQWFYMVLLKGIFGSQADALLTSIRDVMKNSLSDVHFPLEKIIDRYKG
                     SNKDLRFDNEYIESLLNIRYGEGRCRALLHLLFPEMNPTEVFHIDHLHPRNHFSNKYL
                     EKLDYIANSPEKLSFYENPEHWDTIPNLHLLNHSQNISKQDTSLKQWLSHSSNNYTPS
                     MLLVSDDNIEFSRFPEFYNERRNALKQRLLNRVFLTTKIDSSPSTMDTDEEILTD"
     gene            complement(5704..6015)
                     /locus_tag="POA77_004587"
     CDS             complement(5704..6015)
                     /locus_tag="POA77_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367913.1"
                     /translation="MIIDKEYALVDATARLNTDLRDYEHEINNAAIITFGNDLIEVIV
                     YQFSFIISIRAEGEKIKHGLLVNFGKNIARQVSSLCASAMRVYPNEKHKPSRQLFHCI
                     N"
BASE COUNT         1572 a         1282 c         1320 g         1855 t
ORIGIN      
        1 gggtatcggt accgttggat atcacaaaca gttgcaggta tttgaacagg gagttatcgc
       61 tgttaaagct ttccttgctg taacggtgta tctggttaaa ggcttcacga atcgccacgc
      121 cacgcttttt gagttcgatt tgcaccagcg gcaggccgtt taccagaatg gtgacgtcat
      181 aacggttagc gtgtgagccg gtctgctcaa actgctgaat gacctgcacc ttattgcgca
      241 gcaggttctt tttatctatc agatagatgt tttccagacg accatcatca aatgtgaagt
      301 cacagatata gtcgatatgg attttacggg ttttgtccag gctactgtca ctggggctgt
      361 cgagatactg ctctgcgaaa cgccgccatt cactttcgtt aaactccaca ccattaagac
      421 gctgaagctg gtcccgcaca ttcgccagca tcgctgtcgg tgacttaacg gagataagct
      481 catagccctg attaagaagg tcctgaatca gctcccgctc aaggtccaat tcgctctggt
      541 agctgtcacc ggtatccata actttggtga atttatcaag gacgataaag ttattggatt
      601 cagcaatagt gtgtgtttga tgagtcatag cgcatccttt gtgccgtctg gcaagggccg
      661 gaagggggga atggtgactt ccggcgcgta aaaaatagtc tatatactga ccgggtgtta
      721 aggtggcccg atcggtagca atggtcaatt aattactgac agtttcaggt ttcgggaaac
      781 tgaaaagtaa atcacggtag tattcgtatt gtttctggcg cagctcgatt tcgcgaggca
      841 ggccttcatt gatagggttg gtcagtgtgt cgaacttatc gaggataaag gcaatttttt
      901 gttgctcatc aatcgatggt aaaggaatag ataaaccatc aatgttattt gctttaagat
      961 aaccttgccc tgaattgttt tttactgact taaagtaaaa tttcattctt ggtgagtcga
     1021 agaaatgcag aaaatatcta gggtgaagta attcctcatt caacctgatt aggtacatgt
     1081 tttgaccgta aaaatcagga tcgagacatt ttactcgagc aatctcacca atagtaccaa
     1141 tcatgcttat aagaaagtca cctttcaaga tcggtctatt tttggagatg ctcaaaaaat
     1201 cgtcccggga aatatgctta actctttcaa aatcgatgtt cccaccacta atattctttg
     1261 aagtgacata tggaatttca ccattatcat caaacttcgg agttgcgggc ataatccaaa
     1321 atttatctac tagcacctcc tccagagtct tccactcaac ctcaccctct tcaaaactca
     1381 gcaacaggtc gcgatagtag ttgtactgtt ttttacgcat ggtaagttca gctgtaagtt
     1441 cagctgtaag tgcagtgaat ttatccagaa ttcggacgat ttcagactgg atggcaaggg
     1501 acttttctgg gttgttgggg cagggaattg ggatctcagc cattccaaga ccttttgcat
     1561 taattgatga aattttgccg gatgaaacgc ctcttttaat ttgctcttta aataagttcg
     1621 ttctcaggaa gtaagaaaca tactttgaat ttaattcgct tttgaatgaa aaacatgcat
     1681 cgtgaaagac cacatcattt ttcccaagcc atgcagtacc gtttccaata tcctcaattg
     1741 tttcgcctgc agcgacaata attacgtctc catgattagc gacacgtaat ttttcagcga
     1801 ctccttcgtc aaggaatgac ttggtttctt ctgccgatat tccataatgt gtgtacattt
     1861 ctccatagtg gatgcatgga acaccttccg aaataaggtc tgattttacg aacctcttac
     1921 ctcgcagaaa ctggccaaca ctatctagtg tcctccactc aacctcagcc ccatccagca
     1981 gtttttccag ataactcagc ccgctcatgc ctgtacctca ctgccttcaa tttctgccac
     2041 aatcgcgtca atgtcctggc gtaactggtc gattttgctg acggtggttt tcagctcggc
     2101 attcagttca gtgatattga caatttcgcg ggtatctttg gctgccacat agctgctaac
     2161 cgacaggtta tagtcattag acgccacagt ttcataagga accgtttttg ccagatggtc
     2221 agtatctgct ttgctgtcga acacactcat aatctgtctg atatggtcat cagtcaggat
     2281 gttgttatta gtttcttttt taaacagctc actggcatca ataaactgca cgttggtatc
     2341 ggttttgtgc tttgacagca ccaaaatatt caccgcaatg gtggtgccga agaacaggtt
     2401 tggtgcgagt gaaatcaccg tctcaacata gttgttatcg accagatact gacggatttt
     2461 ttgctcagca cctccacggt agaaaatacc tgggaagcag acaatcgccg cgcggccttt
     2521 ggcggacagg tagttaagcg catgaagcac aaacgcaaag tcagcttttg attttggtgc
     2581 cagcacacct gccggagcaa aacgctcatc gttaatcagc gtcggatcgt cactgccaat
     2641 ccatttcacc gaatatggcg ggttcgaaac aatcgcatca aacggttttt catcgccaaa
     2701 gtgaggctca gtcagagtat ttcccagtct gatatcaaat ttgtcgtagt tgatgttgtg
     2761 caggaacatg ttcatacgcg ccaggttata ggtcgtatgg ttgatttcct gaccaaaaaa
     2821 accttcttca atgatatggt tatcaaagtg cttcttcgcc tgaagcagca gtgaaccaga
     2881 gccagcggca gggtcataga ttttgttgac gtgagtctga ccgtgcatcg caagctgagc
     2941 aatcagcttg gagacgtgct gaggagtgaa gaactcccct ccagacttac cggcatttgc
     3001 cgcatagtta gaaatcagga actcgtaggc atcaccaaac aggtcaatct ggtgctcgtt
     3061 aaaattcccc aggttaagcc cttcaacgcc tttcagaacg gcggcaagac ggctgttttt
     3121 gtctttaact gtgttaccca gtcgattgct ggtcgtatcg aaatcggcga acaggccttt
     3181 gatgtcagcc tcggacggat agccgtaggc agagctttca atagcgacga agatactgtt
     3241 taaatcagca ttcagcctgt cgttggtgtt cgctttagcg gcaacgttgc agaacagctg
     3301 gctcgggtag atgaaatagc ctttagtcct gatggcatcg tctttgatgt catcggtaat
     3361 gatgctgtca tcaagtgctg cataatgtat gctctcatcc cctgcttcca tgtagctgga
     3421 aaaattctca ctgatgaagc ggtagaaaag tgcaccgagt acatactgtt taaaatccca
     3481 cccatctacg gagcctctga catcattggc aatggcccag atttgacggt gtagctctgc
     3541 acgctgctga aggcttgtca tcttgtatcc tgttgtatga ttcttaagta ctgttattgg
     3601 cccgatttta tcgcaatttc atattgatga caggctttgt atgtcatacg tttcactgag
     3661 agttcgtttt cccggttgtg tagccaacac tctttcagtc aggccagtag aaacgttgcg
     3721 ctgacgataa atactgaagc cccaatacca tcggggctga acgttcagtc ggtgagaatt
     3781 tcttcatccg tatccattgt ggatggtgat gaatctattt ttgttgtaag aaatacccga
     3841 ttcagtagtc tttgcttcag ggcgtttctt cgctcattgt agaattccgg gaagcgactg
     3901 aactcaatat tgtcatctga aactaacagc attgatggcg tatagttgtt tgatgagtga
     3961 gatagccatt gtttcaggga tgtatcctgt ttacttatat tctgagagtg attcagtaaa
     4021 tgaagattag gtatggtgtc ccagtgttcc ggattttcgt aaaagctgag tttctccggt
     4081 gaattcgcaa tataatctaa tttttcaaga tatttatttg aaaaatgatt tcttggatga
     4141 aggtgatcaa tatgaaacac ctcggtcgga ttcatttcag ggaacagaag atgcaatagt
     4201 gcgcggcagc gaccttcgcc atatctgata ttgagaaggc tttcgatgta ttcgttgtca
     4261 aatctgaggt ccttattcga gcctttgtac ctgtcaataa tcttctcaag aggaaaatga
     4321 acatctgaaa gactattctt cattacgtct ctgatgctcg tgagtagcgc atctgcctgg
     4381 cttccaaaga tccctttcag aagtaccatg taaaaccact ggctaattac tgagcgttca
     4441 ttgtggtttt tattcaggag attaatcgtt gtatataatg ggtgtccatt ggtttgcttt
     4501 ttgtaaagcc agtagaccac aggaatgact gcatttttag atatcaaaga ctgtggattg
     4561 atgccgaatc gcctgataag aacaaaagtc tccttgatac aggattttat ttcagaccat
     4621 tgttgttgta tcttaccgac ctcctctgaa gtgaagtttt ttactttgaa ccggacgtca
     4681 gagtcaataa gcatcaggct ggtttttaag aaccagtctc tttcaatata aaaccccatt
     4741 tcattattct gatgaatgtt ttttgttagt tcatccagtt ctcttctgaa atcaccctgc
     4801 cagtgtgcta cagctattga catcagtaag tcggagaact caagttttgt ccccccgcta
     4861 ttcgtgcgga tgaaaacatc cagcacatga tcaatgtcct ggctgctctc attgaagtag
     4921 tggataagat tctcagttct tatcttggta taaagtttaa gcagggtttt tctggagaat
     4981 tcactggaaa tgagtcctct tttttcgagt tctggtacga caactttaaa taagatatca
     5041 tccggagaat caatttgctc gtatttgaat atttcgtgaa ggcagaacca gtgatgtttg
     5101 ttatcggtaa gtgaatcagt gtattccttg tcggtgagga acctgaagtt gtacttcatc
     5161 atgggctcat cgtctgagtc aagtggcgct gttaaatcga cgtaaagctt tctgggcggc
     5221 aggatgcgat catcctgtgc cgaaggccac cactcccggg gctttttata cgcatacgtt
     5281 ccacacagtc caatatataa agatgtgaga cgctgctgac catcaatcac cgccttaaaa
     5341 tcatgaaatc ccgcatttgt tggtacatat gggttttctt cattaaaccg ctgacagtat
     5401 tctttcagga actcataaaa tttgtaatcg tttttaatac tggcactacg gatatcccac
     5461 atcatgaaag agtttatcgg gtagtctcgc atgatggaat caaatagtag acatatctgc
     5521 ctgtaactcc agacaaactt tcgctgaatt gcaggcagaa gatagtggcg taaattaatt
     5581 gaatctatcg cttgttttat cgttattgct ttttcatact ggccagccat cataaacctc
     5641 atatgttata ttagctggcc tattttaatt taaagcgtgt cggtagtaca gtctttgcac
     5701 aaactaattt atgcagtgga aaagctgacg gctcggtttg tgtttttcgt tagggtagac
     5761 tcgcattgcg gatgcacaaa gtgatgaaac ctgtcttgct atatttttac cgaagttaac
     5821 aagaaggccg tgttttattt tttcaccctc tgccctgatg ctgatgataa agctgaactg
     5881 atatacgatt acctcaatga ggtcattgcc aaatgttata atggcggcgt tgtttatttc
     5941 atgttcataa tccctcaggt cagtgttaag tcttgctgtt gcatctacaa gtgcgtattc
     6001 tttatcgata atcataattt tctcactta
//
LOCUS       DAMZII010000216         5899 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZII010000216 DAMZII010000000
VERSION     DAMZII010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.218"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            139..585
                     /locus_tag="POA77_004588"
     CDS             139..585
                     /locus_tag="POA77_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDI6367914.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            596..2047
                     /locus_tag="POA77_004589"
     CDS             596..2047
                     /locus_tag="POA77_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDI6367915.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2037..3107
                     /locus_tag="POA77_004590"
     CDS             2037..3107
                     /locus_tag="POA77_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6367916.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3107..4855
                     /locus_tag="POA77_004591"
     CDS             3107..4855
                     /locus_tag="POA77_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDI6367917.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLSKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4905..>5899)
                     /locus_tag="POA77_004592"
     CDS             complement(4905..>5899)
                     /locus_tag="POA77_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDI6367918.1"
                     /translation="LGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETPAQ
                     PVVDEQAVFDEKMDVYIKCYNKLQIPVQSNLARYADWLKDFKQGPTGEERTVYGIYGI
                     SESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDD
                     AFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSL
                     AVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQL
                     EAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1367 a         1496 c         1500 g         1536 t
ORIGIN      
        1 aaccattatg tgcattactc cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt
       61 ttaccgacat atcgcctctc atcacttaat cgctccacca ttacaattga ttaaaatata
      121 ttttatggag tccgattaat ggcagctctt actgcaagct gtattgacct gaatattcag
      181 ggcaatggcg cttattccgt tctgaagcag ttggcgacaa tagcgttaca aaacggtttt
      241 atcaccgact cacaccagtt cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact
      301 ggatttggtt ccggtgtcgc cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttc
      361 gtattattcg cccgcaaagc gcaggctatt gactggaaag ccagcgatgg cgaagacgtc
      421 aattgctgga tctgcctcgg cgtgccgcaa agcggcgaag aggatcaggt caaaatcatc
      481 ggcacactgt gtcgcaaaat tattcacaag gaatttattc atcaactgca acagggcgat
      541 accgaccagg tgcttgcctt gttaaatcaa accctcagct cataaggaag tggcgatgga
      601 gtcatcctta cgtattgtcg cgatcaccaa ctgccccgcc gggatcgctc acacctacat
      661 ggtggcggaa gccctggaac agaaagcccg ttctctcggt cataccataa aagtggaaac
      721 tcaagggtcc agtggcgttg aaaaccgctt atccagcgaa gagattgccg ctgccgatta
      781 cgtcattctc gctaccgggc gtggcctgag cggcgatgat cgcgcgcggt ttgccgggaa
      841 gaaagtttat gagattgcca tctcccaggc gttgaaaaat atcgaccaga ttttcagcga
      901 attaccgaca aactcgcagc tttttgccgc agatagcggc gtgaagcttg gcaaacagga
      961 agtgcaaagc ggcagcgtga tgagccacct gatggcgggc gtctctgcgg cgctgccgtt
     1021 tgtcatcggc ggtggtatcc tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc
     1081 gtataccgat atgtcgaaag gcgcgccgtc gtttacctgg gtagttgaat ccatcggtta
     1141 ccttggcttc acctttatga tccccatcat gggcgcttat attgcctcgt cgattgccga
     1201 taaaccggct ttcgctccgg cgtttctggt ttgctatctg gcgaacgaca aagcgctgct
     1261 cggcacccag tcgggtgcgg gattcctcgg cgcggtggtg ctggggctgg cgattggcta
     1321 tttcgtcttc tggttccgca aagtgcgcct tggcaaagca ttacaaccgc tgctcggttc
     1381 catgctgatc ccgttcgtta ctctgctggt tttcggcgtg ctcacttact acgttatcgg
     1441 gccggtgatg tccgacctca tgggcgggct gctccacttc ctgaatacca ttcctccatc
     1501 aatgaagttt gcggcagcgt ttctggtcgg tgcgatgctg gcgttcgata tgggcggccc
     1561 catcaataaa accgcctggt tcttctgctt ctcactgctg gaaaaacaca tttatgactg
     1621 gtacgccatc gtcggcgttg tcgcgttgat gccgcccgtt gcggcgggtc ttgcgacgtt
     1681 cattgcgccc aaactgttta cccgacagga aaaagaggct gccagtagtg ccattgtggt
     1741 cggtgccaca gtcgcaaccg aaccggctat accttacgtt ctggcagctc cgttgccgat
     1801 gattaccgcc aatacgctgg cgggtggcat taccggcgtg ctggtgatcg ctttcggaat
     1861 caaacgtctg gcacctggtt tgggtatctt tgacccgctg atcggcctga tgtcgccggt
     1921 gggttcgttt tatctggtgc tggcgattgg cctcgcgctg aacatttcat tcattatcgt
     1981 cctgaaagga ttgtggttac gccgcaaagc gaaagccgcg caacaggagc ttgtccatga
     2041 acattgagtt actgcaacag ttgtgcgaag ccagcgccgt cagcggcgat gaacaggaag
     2101 ttcgcgacat tctgataaac acgctggaac cttgcgttaa tgagatcacc tttgatggtc
     2161 tgggcagctt tgttgcccgt aaggggaata aaggtccaaa agttgccgtt gtcgggcata
     2221 tggatgaagt cggctttatg gtcacccaca tcgacgagag cggttttctg cgtttcacca
     2281 ccattggtgg ctggtggaat cagtcaatgc tcaaccaccg ggtaaccata cgcacacaca
     2341 agggatttaa aatccctggt gtgattggtt ccgtcgcacc tcatgcgtta acggaaaagc
     2401 aaaagcaaca gccgctgtca tttgatgaga tgttcattga tattggcgcg aacagtcgcg
     2461 atgaggtgga aaaacgcggt gtggcgatgg gcgattttat tagcccggaa gccaattttg
     2521 cctgctgggg cgaagataaa gtggtcggca aggcgctgga taaccgcatc ggctgcgcga
     2581 tgatggcgga gctactacag acagtaaata acccagaaat tacgctgtac ggcgttggca
     2641 gtgtggaaga agaagttggg ctacgcgggg cacaaacctc ggcggaacac attaaaccgg
     2701 atgtggtgat tgtgctggat accgctgtcg caggtgatgt tccgggcatt gataacatta
     2761 aatacccgct gaaactgggc caggggccgg ggctgatgct gtttgacaag cgctacttcc
     2821 ccaaccagaa actggtagca gtgttaaaaa actgtgccgc acataacgat ttaccgctgc
     2881 aattttccac catgaaaacc ggagcgacgg atggcgggcg ctacaacgtg atgggcggcg
     2941 ggcgtccggt tgtcgcgctg tgtctgccaa ctcgttatct gcacgctaac agcggcatga
     3001 tttcaaaagc cgattacgat gctctgctca cgctgatacg ggattttctg acgaccttaa
     3061 ctgcggagaa agtcaacgcg tttagccagt tccgtcaggt ggattaatgc taaacgaacg
     3121 ccagttaaag attgtcgatc tgctggagca acagccgcgc acgcctggag agctggcgca
     3181 acagactggc gtttcaggca ggaccatcct gcgtgatatt gactatctca acttcaccct
     3241 taacggcaaa gcccgcattt tcgccagtgg tagtgcgggc tatcagctgg aaatcttcga
     3301 gcgccgcagc ttttttcagt tgctgcaaaa gcacgataac gacgatcggc tgctggcgct
     3361 attattacta aataccttta ctccccgtgc gcaactcgcc tcggcgctta atttgccaga
     3421 aacgtgggta gcagaacgtc tgccccggtt aaaacagcgt tatgaacgca cttgttgcct
     3481 ggccagtcgc cccggtttgg gccatttcat tgatgaaaca gaagagaagc gcgttatctt
     3541 gctggcgaac ttgctaagca aagatccgtt tttaattccg ctggcgggca taacacgaga
     3601 caaccttcag catttatcca cggcctgcga caaccaacac cgctggccgc tgatgcaggg
     3661 tgattatctc tccagcctga ttctggcgat ttacgccctg cgtaatcagc ttactgacat
     3721 atggccgcaa tatcccggta acgagataaa acaaatcgtt gaacagtgcg gcctgtttct
     3781 cggtgataac gctgtaagaa ccctgacggg tttgatagag aaacagcatc agcaagcgca
     3841 ggtaatttca gccgatcatg tgctggggtt gctgcaaagg gtgccaggca tcgcgtcatt
     3901 gaatattatt gatacgcagt tggttgagaa tattaccggg catttattac gttgccttgc
     3961 cgcaccggtg tggattgctg agcaccgcca aagcagcatg aataacctga aagccgcctg
     4021 gcctgcggcc tttgatatga gtctgcactt tattacgcta ctgcgtgaac agctcgatat
     4081 tccccttttc gacagcgatc tgatcggttt gtattttgcc tgtgcgctgg aacggcatca
     4141 aaacgaacgc cagccgatca ttttgctctc ggaccagaac gcgattgcca ctattaatca
     4201 gctcgccatt gagcgcgatg ttttaaattg tcgggtaatt attgcccgta gcttaagcga
     4261 acttgttgcc attcgcgaag agattgagcc gttattgatc attaacaata gccattattt
     4321 actggaagac gcggtaaata attacatcac cgtaaaaaat atcattacgg ctgccggtat
     4381 cgaacaaata aaacattttc tggcgacggc atttattcgc cagcaaccgg agcgtttttt
     4441 ctctgccccc ggaagttttc attattcgaa tgtacgcggt gaaagctggc aacatattac
     4501 ccggcaaatt tgtgcgcaat tagtggcaca acaccatatt accgccgatg aagcacaacg
     4561 catcatcgcc cgcgaaggcg aaggtgaaaa cctgattgtt aatcgcctcg ccatcccaca
     4621 ttgctggagc gaacaggagc gacgttttcg tggatttttt attaccctcg cccacccgat
     4681 cgaggtgaat aacgaaatca ttaaccatgt gttgattgcc tgcgccgccg ccgatgcgcg
     4741 tcatgagcta aaaatattta gctatctggc aagcgtattg tgtcagcatc cggcagaggt
     4801 tattgccggg ttaacaggat atgaggcatt tatggagtta cttcacaagg ggtgaactgt
     4861 gtcggatgcg ccgcatccga cactgcattt atcatcaaaa acgtttaacg caggctatta
     4921 tagtcatcaa ccatttcgtt gtactcgcgt aatgctctcg ggaaagagtc gtcgaccatc
     4981 cagcttgagt ttgcgtcctg cagttgctct ttatcccagt cagagtacgg gactttatct
     5041 ctgatgcggc gaacgtattt tttagcctcg agttgatact ggcctgccga attaataaac
     5101 gagaaattca tgccgccttt atccgcttct ttggcctgcg ccactagcgt ttccagttca
     5161 gacactttct tcattgccgc atcaacatca aacttctctt gcgatatcag attattaatt
     5221 tgtttcgctg aaatcatgac tgccagagag tagtagtgga atgttttccc ttctctttct
     5281 tcaatatttt tcagttccgc aagctggcgc ttatcgttaa tttcctgaat cgccgcatga
     5341 taagattctg ctacagcttc aaaggcttcc agatttttta agaatgtctg gtgcagcgtt
     5401 ttccctttcg caaacgcatc gtctttataa ttttcctggg tgtaatattt atccatttcg
     5461 ttaatggtat tacccaacgc cacggcagca tcgatataac tcaccgccac gccatcaata
     5521 ggttgcagcg caggcgttaa cgccacagca ctttttacgc ctttttcaca ctcagcgagg
     5581 ttggattcac taatgccgta gatgccataa acagtacgct cttcaccggt aggcccctgt
     5641 ttaaaatctt tcagccagtc agcataacgc gccagattgc tctgtacagg gatctgtaac
     5701 ttgttatagc acttgatata gacatccatt ttttcgtcga aaaccgcttg ttcatcgacc
     5761 accggttgcg caggtgtttc aggtttagct tctgcttttg caacgggagc ttcaggagct
     5821 ggtgcggcag gttgactatt ggcaggcggg ggcgtttctg tttcggcttt tttatcatca
     5881 caacccgtca gaccgaggg
//
LOCUS       DAMZII010000217         5848 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZII010000217 DAMZII010000000
VERSION     DAMZII010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.219"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            286..1338
                     /locus_tag="POA77_004593"
     CDS             286..1338
                     /locus_tag="POA77_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDI6367919.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1735..1902)
                     /locus_tag="POA77_004594"
     CDS             complement(1735..1902)
                     /locus_tag="POA77_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367920.1"
                     /translation="MGRTLEEVIQQEKPEVVDAAKVKAKEILKEIETSKPSISSDKLD
                     TSSDNQSTASD"
     gene            complement(1964..3163)
                     /locus_tag="POA77_004595"
     CDS             complement(1964..3163)
                     /locus_tag="POA77_004595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367921.1"
                     /translation="MNSVKSLSPQSDESVQGFIFRVMLLNGWGDFTTLIAHGGWGSEP
                     SAPFESKQFFNVFDAYLLLNVFEKQLDKYTEYSLFSDRFSHINLFRETFFPHKRKASC
                     GSSIPLRFCRFCIEEQLYDKGFSYFKLEWLTDTFCKTHLTPMYEFKKSSSLAKIKNAL
                     MSVLLADWEAVEDLLSTPSFCGEPRQRSSLSLSRDERVVSFSPCAQRILVGYFMSHSD
                     YYPTGCFSVADYGFLSAIQRKYFSKNQRRAQLTKNLEEVYEYEISSNYQRLMDFNVKN
                     LELKQIRHFGKLHWLIKSKGRSCKFCFKHGSDDFRLCPAAGLIYYSGKGFSSKENLCD
                     KSFERLELDIEFHQRRLKISDGERVVCNSIDKSNHIAAVGGPEIYQRERELRLKAAAK
                     ALDSLIF"
     gene            3310..3897
                     /locus_tag="POA77_004596"
     CDS             3310..3897
                     /locus_tag="POA77_004596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6367922.1"
                     /translation="MKLKQIATINAGYPFRGKIPEVPGSAVVAVQMKDVSLTEGIRWS
                     DCLETTLPGKREPDYLTTGDILVAARGSHNYAVQVDQLLASTGKQAVAAPHFFVVSLK
                     KKDILPEFMVWLLNQAPAQRYFEQNAEGTLTKSIRRSVLEDAPVVVPPFAKQRAIIAM
                     ANTLGEEQRLIQRLVNNGERMMGAIANDLYQAHGH"
     gene            3970..5493
                     /locus_tag="POA77_004597"
     CDS             3970..5493
                     /locus_tag="POA77_004597"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6367923.1"
                     /translation="MNDKINQDSINKALWNACDTFRGTISADTYKDFILTMLFLKYIS
                     DVWQDHYDGYKAEYGDESELIEEMMKNERFVLPKAASFYSLYERRHEPGNGERIDQAL
                     HAIEEANGTKLKDAGKSVFQDISFNTDKLGEEKQKNTILRHLLEDFAKPELNLKPSRV
                     GTLDVIGNAYEYLIKNFAASGGQKAGEFYTPPEVSDLIAELLDPQPGDSICDPACGSG
                     SLLMKCGLKVVANHGSKQYALYGQEAIGSTWSLAKMNMFLHGEDNHKIEWGDTIRNPK
                     LLDKNGDMMLFDIVTANPPFSLDKWGHDEAEHDKFSRFRRGVPPKTKGDYAFILHMIE
                     TLKPKSGRMGVVVPHGVLFRGSSEGKIRQQLIDENLLDAVIGLPEKLFYGTGIPAAIL
                     LFKKNKSDDNVMFIDASRDFKAGKNQNLLTEENIAKIVATYHAGNDVDKYAYVASLEE
                     IKENDYNLNIPRYVDTFEEEEEIDLMAVRAEREQLKAQLTELETEMAKYLEELGYGA"
     gene            5483..5725
                     /locus_tag="POA77_004598"
     CDS             5483..5725
                     /locus_tag="POA77_004598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367924.1"
                     /translation="MVPNGWALQDFNSLKIATIDGDRGKNYPKKEDYLQIGHALFLGS
                     SRISGECLSDFQKEVFGIPVAIAHSLQQFDFVVHPL"
     gene            complement(5612..>5848)
                     /locus_tag="POA77_004599"
     CDS             complement(5612..>5848)
                     /locus_tag="POA77_004599"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6367925.1"
                     /translation="NLWWQQVRESGIRPLLQFGQRLKNYLHGIVASAVHPLHTCRLEG
                     MNNKIKLLKRMGYGYRDTEYFFLKVREAFPGDPR"
BASE COUNT         1655 a         1339 c         1287 g         1567 t
ORIGIN      
        1 cgatgatttt aagcaccgag actatccggt cttttactaa ataatggaaa acaatccatc
       61 cctggaagat aattgtaaaa aaaacaacag aataagaatt aatcttagga taaatattta
      121 tttatcatgg ccttttaacc ggtaaaaaat ggcactcggc acagtaaacc ccgctatagt
      181 tatgctatgc atttgtcttt aatgatatct cgtcgttata ttaacaggct gaaattatca
      241 ttaatgcaca tttcattatt agcagggata cctgaggagt ttaaagtgaa catatatatc
      301 gggtggcttt tcaaattaat ccctttgatt atgggattaa tttgcatcgc gttaggtggc
      361 tttgtgctgg aaagttcagg gcaaagcgag tatttcgtcg cgggtcatgt gctgatttct
      421 ctggcggcca tatgcctggc attattcact accgcattta ttatcatttc gcagctcacg
      481 cgcggcgtta atacgtttta caatacattg ttccccatta ttggctatgc ggggtcaatt
      541 atcaccatga tatggggttg ggcactgtta gcaggcaatg atgtgatggc agacgagttt
      601 gtcgccggcc atgttatttt cggcgttggt atgattgccg cctgtgtatc gacggtggca
      661 gcgtcatccg gtcactttct gctcattccc aaaaatgcag cggggagcaa gagcgatgga
      721 acgccggtac aggcttattc ttcattaatc ggtaattgcc tcattgccgt tcccgtttta
      781 ctcaccctgc tcggtttcat ttggtctatt acgctgttac gtagtgctga cataactccg
      841 cattatgtcg cgggtcacgt attgcttggg ttaaccgcaa tctgtgcctg tctaattggc
      901 cttgttgcca caattgtcca tcaaacacgt aatacgtttt caactaaaga acactggctg
      961 tggtgttatt gggttatttt tctcggctca atcacggtac tgcaggggat atacgtctta
     1021 gtcagttccg atgcaagcgc ccgactggct cccggcatta ttcttatttg cctcggaatg
     1081 atctgttaca gcatattctc aaaagtctgg ctactggcac tggtatggag acgtacctgt
     1141 tcgttagcca acagaatacc gatgattccc gtcttcacct gcctgttttg ccttttcctg
     1201 gcatcgtttc ttgcggaaat ggcgcagacc gacatgggat attttattcc ttcgcgggtt
     1261 ttggtcggtt taggagcggt atgctttacg ttgttctcaa tcgtttcaat attagaagca
     1321 ggttctgcta aaaaataatt gcaacgtacc ggataaaacc agcgttgacc atttgcgtaa
     1381 cgctggtttt tcttaggcat catgaaataa cgcagcatta atgcatagtg gttaaggata
     1441 aaaaaacaac aaagttactg tttttttcaa cctgttcata ttttataaag atctggccac
     1501 tgggtgagtt ttcaatcacc tttccttcca ccttatatta agcctggagg gtttcagttc
     1561 acgggctcat tagaaaataa tcacaagata gccactatca aaattagctc atttttaatg
     1621 cggctctaat aattttcatc tttaggtaac aggtcaggac gacttcgctg caattgtgta
     1681 gtgacaataa agtttgtaca ctaatcgcaa atttggaaca ataaagtttg tacactaatc
     1741 agatgctgtg gactggttat cacttgatgt atccagcttg tcggatgaaa tgctaggttt
     1801 tgaggtttca atttccttga gaatttcttt agctttaact ttggcagcat caacaacttc
     1861 gggcttctcc tgttggataa cctcttccag cgttcttccc atggtacatc cctcgctcaa
     1921 gatcaattag ttacatgcta gaaaagtata gcatcgtagg tacttaaaaa atcaatgaat
     1981 ctagtgcttt tgctgctgcc tttaagcgta actcacgctc tctttgataa atttccggcc
     2041 ctccgactgc agcgatatga ttgcttttat caatactgtt gcaaacaaca cgctcgccat
     2101 cagatatctt caatcgcctt tgatgaaact cgatgtctag ttcaagtctt tcaaatgatt
     2161 tatcacatag attttctttt gatgaaaacc cttttcccga gtagtagata agaccagcag
     2221 caggacacaa cctaaaatca tctgaaccgt gcttaaaaca gaacttacat gacctacctt
     2281 tagatttaat caaccagtgc aatttgccaa aatgacgaat ctgcttcaat tccaaattct
     2341 taacattgaa gtccattaat ctttgatagt tagaactaat ttcatattcg tatacctctt
     2401 caagattctt tgttaattga gctcttcttt ggtttttaga gaaatacttt ctttggatgg
     2461 cagagagaaa tccatagtct gctactgaaa aacaaccggt aggatagtaa tcagaatggc
     2521 tcatgaaata tccaaccaga attctttgag cacacggaga gaagctcacg actcgttcat
     2581 ctctagataa actcaagctg cttctttggc gcggctcacc gcaaaaggaa ggggttgaca
     2641 gtagatcctc aactgcctcc cagtcagcta ataaaaccga cattaatgcg ttttttatct
     2701 ttgccaatga agagctcttc ttgaattcat acattggcgt taagtgagtc ttgcagaacg
     2761 tatccgtaag ccattccaac ttaaaatatg agaacccttt gtcatagagt tgctcctcaa
     2821 tacagaaacg acagaatcgc aatggtatcg agcttccaca agatgctttt cttttatgag
     2881 ggaagaaagt ttctcgaaaa agatttatat gtgagaacct atcgctaaac aaactgtatt
     2941 cagtatattt atctaactgt ttttcaaaaa cattcaataa taaataagca tcaaaaacat
     3001 taaagaactg cttggattca aatggtgcgg acggctcgct tccccagccc ccatgagcaa
     3061 taagagttgt aaaatcgccc cagccattta ggagcataac cctaaagata aatccctgaa
     3121 cagactcatc cgattgcgga cttaaacttt taacactatt cattaagcaa ttaatttaat
     3181 taaaattctg tagaatcagg gcaaagaaaa ataaattttt gcatcaaagg cgatgaaaca
     3241 actttagctt gacatttaac caggatcaac agaatatttc tgcatcattg actatgaaca
     3301 ggtgaaggtt tgaagctcaa acagatagca accatcaatg caggctatcc atttcggggc
     3361 aaaatccctg aagtgccagg ctctgctgtg gtcgcggttc agatgaagga tgtctcgcta
     3421 accgaaggga ttcgctggtc agactgtttg gaaaccacgc tccccggcaa gcgcgaaccc
     3481 gactacctca ccacaggtga tatcttagtg gccgctcgtg gcagccataa ctacgccgta
     3541 caggtggatc agttattggc cagcacaggt aagcaggccg ttgctgcgcc gcactttttt
     3601 gtggtcagcc tgaagaagaa agacattctg cccgagttta tggtgtggct actgaatcag
     3661 gctccggctc agcgttattt tgagcagaat gctgaaggca ccttaacaaa aagtattcgc
     3721 cgtagtgtgc tggaagatgc cccagtagtt gttccaccat ttgccaagca gcgagcaatc
     3781 attgcgatgg ctaacacttt gggtgaagag caaaggctga ttcagaggct agtaaacaac
     3841 ggtgagcgca tgatgggcgc aatcgccaac gacttgtacc aggcgcatgg tcactaaaca
     3901 gccagcacaa cgaatttaag acttgaactg ggccagccac taattggccc cactaaaagg
     3961 aaacaatgga tgaacgataa gattaaccaa gacagcatta acaaagcgct gtggaatgcc
     4021 tgcgatacct ttcgtggcac catcagcgcc gacacataca aagacttcat cctgactatg
     4081 ctgttcctca agtacatctc agacgtatgg caggatcact acgatggtta caaggccgaa
     4141 tacggtgacg agtcagaact tatcgaagag atgatgaaaa acgagcggtt tgtgctgccc
     4201 aaagcggcca gcttctactc cctgtacgaa cgccgtcacg aacccggtaa tggtgagcgc
     4261 atcgaccagg cattgcacgc cattgaagaa gccaacggca ccaaactcaa agacgctggc
     4321 aagagtgtgt tccaagacat cagctttaac accgacaaac tgggtgaaga gaagcaaaag
     4381 aacaccattc tgcgccatct actggaagac ttcgccaagc ccgagttgaa cctaaaaccc
     4441 agccgtgttg gcacactgga tgtgatcggt aacgcctacg aatacctgat caaaaacttc
     4501 gccgccagcg gtggccaaaa ggcaggtgag ttttataccc cgccagaagt cagtgacctg
     4561 atcgccgaac tgttagaccc gcagccgggc gacagcatct gtgacccagc ctgtggctct
     4621 ggctcgctgc tgatgaagtg cggcctcaaa gtggtagcca accacggtag taagcaatac
     4681 gccctatatg gtcaagaagc catcggctcc acctggtcgc tggccaaaat gaacatgttt
     4741 ctgcacggcg aagacaacca caaaattgaa tggggcgaca ccatccgcaa ccccaagctg
     4801 ctcgataaaa atggagacat gatgctgttt gatatcgtga cagccaaccc accgttttcc
     4861 ttagataaat ggggccacga cgaagccgaa cacgacaagt tcagccgctt ccgccgcggt
     4921 gtaccgccaa aaaccaaagg cgactacgcc tttatcctgc acatgattga aaccctgaaa
     4981 ccaaaatcag gacgaatggg cgtggtggtg ccacacggcg tgctattccg tggctcgtct
     5041 gagggaaaaa ttcgccagca actgattgac gaaaacttgc tggatgccgt gattggactt
     5101 ccagaaaagc tgttttacgg cacgggcatt ccagcggcaa tcctgctctt taagaaaaat
     5161 aagagcgacg ataacgtcat gtttatcgac gccagccgcg acttcaaagc cggtaaaaac
     5221 cagaacctgc tgactgaaga gaatatcgcc aagattgtag ccacctatca cgctggcaac
     5281 gatgtcgata aatacgccta cgtcgccagt ctcgaagaga tcaaagagaa cgactataac
     5341 ctgaacatcc cgcgctatgt cgataccttt gaagaagagg aagaaatcga cctgatggcg
     5401 gtacgggcag agcgtgagca gcttaaggcg caactgaccg agctggaaac cgagatggcc
     5461 aagtatcttg aggagttggg atatggtgcc taatgggtgg gctttacaag actttaatag
     5521 cctaaaaatt gcaacgattg acggagatcg tggtaagaac tatccaaaaa aagaagacta
     5581 ccttcaaatc ggtcatgccc tttttttagg ttcatcgcgg atctccgggg aatgcctctc
     5641 tgactttcag aaagaagtat tcggtatccc ggtagccata gcccattcgc ttcagcagtt
     5701 tgattttgtt gttcatcccc tctagtcggc aggtgtgcaa cggatgaacc gctgaagcaa
     5761 ctatgccatg aagatagttt ttaagacgct ggccaaattg gagtagtggc ctgataccgc
     5821 tctctctgac ttgttgccac cagaggtt
//
LOCUS       DAMZII010000218         5823 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZII010000218 DAMZII010000000
VERSION     DAMZII010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.220"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..3779
                     /locus_tag="POA77_004600"
     CDS             <1..3779
                     /locus_tag="POA77_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDI6367926.1"
                     /translation="SSTNAQLLQVGVLGKGELNITTGGIVKARDTQIALNDKSKGDVR
                     VDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGE
                     AAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTV
                     FNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTL
                     TNALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDSTFTLERDNTAALTHAMLQ
                     SDSENTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKG
                     RNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSL
                     TLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQK
                     LTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDA
                     DGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVNKGGISQGELT
                     GGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNTQGLGRGNIANDGLLTLKNVT
                     GELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVINN
                     GLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSDSA
                     INMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENGGTVQMNSEGG
                     KPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQ
                     TVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPD
                     TPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHSQG
                     SASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYA
                     NQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSD
                     NRSADDYDSRGVTASVEGGYTFEAGTFSGSEETLNTWYVQPQAQITWMGVKDSDHTRK
                     DGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNG
                     QTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            3987..4253
                     /gene="hipB"
                     /locus_tag="POA77_004601"
     CDS             3987..4253
                     /gene="hipB"
                     /locus_tag="POA77_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDI6367927.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4253..5575
                     /gene="hipA"
                     /locus_tag="POA77_004602"
     CDS             4253..5575
                     /gene="hipA"
                     /locus_tag="POA77_004602"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDI6367928.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
BASE COUNT         1585 a         1299 c         1631 g         1308 t
ORIGIN      
        1 catcatctac taacgcacaa ttgctacagg tcggtgtatt aggcaagggt gaactgaata
       61 ttaccaccgg aggtatagtt aaagcgcgtg atacacagat agctctcaat gacaaaagta
      121 agggcgacgt gagggtggat gggcagaact ctcttcttga aacattcaat atgtacgtag
      181 ggacatctgg tacgggtacg ttaaccctga cgaatagcgg cacgctgaat gtcgaaggtg
      241 gagaagttta cttaggtgtt tttgaacctg ctgtaggaac gctaaacatt ggtgcagctc
      301 acggtgaggc ggcggcagat gccgggtata taaccaacgc gacgaaagta gagtttggtt
      361 ctggcgaagg tgtttttgtc tttaatcata ctaataacag tgatgccggc taccaggtcg
      421 atatgctgat tacaggtgac gataaagacg gaaaagtgat ccatgatgca ggccatacgg
      481 tgttcaatgc agggaatact tatagcggta aaacgctggt caatgacggc ctcctgacca
      541 ttgcgtctca tacggcagat ggggtaacgg gcatgggttc gagtgaagtc accattgcaa
      601 gccccggtac gctcgacatt ctcgcatcaa cgaacagtgc aggagattac acgctgacca
      661 atgcgctcaa aggcgatggc ttgatgcgag tgcagctgtc atcctacgac aagatgtttg
      721 gctttaccca tgcaacaggg actgaattcg ccggtgttgc ccaactgaaa gacagtacct
      781 tcactctgga acgcgacaac accgctgcgc ttactcacgc gatgttgcag tctgacagtg
      841 aaaataccac atcggtaaaa gtgggagagc aatccattgg tggactggcc atgaatggcg
      901 gtacgctcat tttcgatacg gatattcctg ctgcgacgct tgcagaggga tatatcagcg
      961 tcgatacgct ggttgtcggc gcgggtgact acacctggaa aggccgtaac tatcaggtaa
     1021 acgggacggg cgacgtgctt atcgacgtgc ctaaaccgtg gaatgatccc atggcgaata
     1081 accctctgac gacgctcaat ttgctggaac acgacgatag ccatgtcggc gttcaactgg
     1141 tgaaggcgca aacggttatt gggtcgggtg gctcattaac gttacgtgat ttacagggcg
     1201 acgaggtgga agcggacaaa acgttacaca ttgcgcaaaa cggaacggtg gtcgccgagg
     1261 gtgattatgg attccgcctc acgaccgcac caggtgatgg tttgtacgtt aactatgggc
     1321 tgaaagcgct gaacatccat ggtgggcaaa agctgacgtt agccgaacat ggcggagcct
     1381 atggcgcaac ggccgatatg tcggcaaaaa tcggtggtga aggggatctg gcaatcaata
     1441 cggtgcgaca ggtttcgctt tccaacggtc agaacgacta tcagggggca acctacgttc
     1501 agatggggac attacgtacc gatgcggatg gtgcacttgg caacacccgg gaactgaaca
     1561 tcagcaacgc ggccatcgtc gatcttaatg gatcgacgca gacggtagag acattcaccg
     1621 ggcagatggg ttcgactgtt ttgttcaaag agggggcgct gacggtaaat aaaggtggga
     1681 tcagtcaggg tgaactgaca ggtggcggaa acctgaatgt tacaggggga acgctggcta
     1741 tcgaggggct taatgcacgc tacaatgcgt taaccagcat tagcccaaat gcggaagtca
     1801 gcctcgataa tactcagggg ttaggcagag gaaatattgc caatgacggt ttgttaacgc
     1861 taaaaaacgt gactggcgaa ctgcgtaata gcataagcgg gaagggtatc gtgagcgcaa
     1921 ccgccaggac agatgtagag ctggatggcg ataatagccg ctttgtgggg caattcaaca
     1981 ttgatacagg cagcgcgctc agcgtcaacg agcagaaaaa cctgggtgat gcttccgtta
     2041 tcaataatgg cctgctcacc atctccactg agcgtagctg ggcgatgacg cacagtatca
     2101 gcggtagcgg tgatgtgaca aaactgggta ccgggatcct gactcttaac aacgattccg
     2161 cggcgtatca gggtacgacg gatatcgtgg ggggggaaat tgctttcggt tccgactctg
     2221 ccattaatat ggcaagtcaa cacattaata tccataacag cggtgtgatg tcgggaaatg
     2281 tcaccactgc aggtgatgtg aacgttatgc ctggggggac actgcgtgtc gctaaaacca
     2341 caatcggcgg caacctggag aatggcggca cggttcaaat gaatagcgaa ggggggaaac
     2401 cggggaatgt actgaccgtt aacggcaact ataccggaaa caatggcctg atgacgttca
     2461 acgcgacgct gggcggcgat aattcgccca ccgataaaat gaacgtgaaa ggcgataccc
     2521 aagggaacac tcgcgttcgg gttgataaca ttggcggcgt cggtgcacaa acggtcaacg
     2581 gtattgaact cattgaggtt ggcggtaatt ctgcaggtaa cttcgcgctg accaccggaa
     2641 ctgtcgaagc tggggcttac gtctacacgc tggctaaagg gaaggggaat gacgagaaaa
     2701 actggtatct gaccagtaaa tgggacggcg taacgccacc ggatacgccc gaccccatca
     2761 ataatccccc tgttgtggat ccggaaggcc catcagttta tcgcccggag gccggaagct
     2821 atatcagcaa cattgccgca gccaactcgc tgtttagcca tcgtttacac gaccgtctgg
     2881 gtgagccgca gtatatagat tcactgcatt ctcaggggtc ggcaagcagt atgtggatgc
     2941 gtcatgtcgg agggcacgaa cgttcaaggg ccggtgacgg tcagctaaat actcaggcta
     3001 accgctatgt attgcagcta ggcggcgatt tggcgcagtg gagtagcaac gcgcaggatc
     3061 gctggcatct tggcgtgatg gcaggttacg ctaatcagca cagtaatact cagagtaatc
     3121 gtgtgggtta taaatcggat gggcgcatca gcggttacag cgctgggctg tacgcgacct
     3181 ggtatcagaa cgatgcgaat aagaccggcg cttatgttga cagctgggcg ctgtataact
     3241 ggtttgataa cagcgtcagt tccgataacc gttctgctga cgactatgat tctcgcggtg
     3301 tgacggcctc tgttgagggt gggtatacct ttgaagcggg aacatttagc ggcagcgaag
     3361 agacgctgaa tacctggtac gtccagccac aggcgcaaat cacctggatg ggtgtgaaag
     3421 attccgacca tacccggaaa gacggaacgc gcattgaaac ggaaggcgac ggaaatgtgc
     3481 aaacgcgact tggggtgaaa acctacctga acagccatca ccagcgtgac gatggtaaac
     3541 agcgtgagtt ccagccttac attgaagcga attggatcaa caacagcaaa gtctacgccg
     3601 tgaagatgaa tggtcaaacc gtaggccgtg aaggtgcgcg taatctcggt gaagtacgta
     3661 ccggggttga ggcgaaagta aataacaacc ttagcctgtg ggggaatgtc ggtgtgcaac
     3721 taggtgataa aggctatagc gatactcagg gcatgctggg agtgaaatat agctggtaaa
     3781 ccgtataagc cgcatgtcga gatggcatgc ggcttaatat tgccgacttc aaaggcttta
     3841 aagcctccct tttatccgcg atcgcggata tcgcagcgtt tatcccgtag agcggataag
     3901 acgtgtttcc aaattgactt atcctcacta aaggataaaa ctgataatat cctcttatgc
     3961 ggataaattt gctgtggacg tatgacatga tgagctttca gaagatctat agcccaacgc
     4021 aattggcgaa tgcaatgaaa ctggttcgcc agcaaaatgg ctggacgcag agcgagctgg
     4081 cgaaaaaaat tggcattaag caggcgacaa tttccaattt cgaaaacaac cctgacaata
     4141 ccacgctcac gacatttttt aagattttac agtcgcttga actctcaatg acactatgcg
     4201 acacgaaaaa tgcctcgcca gaatcaacag aacagcaaga tctggagtgg taatgcctaa
     4261 acttgtcact tggatgaaca accagcgggt aggcgagtta acgaagttag ccaacggcgc
     4321 gcacaccttt aagtatgcac cggagtggtt agccagccgt tatgccagac cgttgtcact
     4381 ttcgctgcca ttgcagaggg ggaatatcac ctcagatgcc gtatttaact tcttcgataa
     4441 cctgttaccc gatagcccga ttgtacgtga ccggatcgtt aaacgttatc atgctaaatc
     4501 cagacaaccg tttgatttat tgtcagaaat agggcgagac agcgttggtg ccgtaacgtt
     4561 attacccgaa aatgaaacca taacgcgccc gataatggca tgggaaaagc ttactgaagc
     4621 caggcttgaa gaagtattaa cggcttataa agcagatatc cctctgggca tgattagaga
     4681 agaaaatgac tttcgcatct cggttgctgg cgcgcaggag aagacagcgc tgcttagaat
     4741 aggcaatgac tggtgcattc cgaaaggaat aacgccgacg acgcacatca ttaaattacc
     4801 gattggcgaa atcaggcagc ccaatgcgac gctcgatctc agccaaagcg ttgataatga
     4861 gtattactgt ctgctgctgg cgaaagaact tgggttgaat gttccggacg cagaaatcat
     4921 taaagcggga agggtgcgcg cgttagcggt cgaacgtttt gacaggcgtt ggaatactga
     4981 gcgaacggtt ttacttcgct tgccacagga ggatatgtgt cagacattcg gtttaccttc
     5041 atctgtgaaa tatgaatcag atggaggccc aggcatcgcg cggatcatgg cgtttttgat
     5101 ggggtccagc gaggcgcttc gcgatcgtta tgattttatg aaattccagg tcttccagtg
     5161 gttgattggc gcaacggatg gccatgcaaa aaacttctcc gtatttattc aggctggcgg
     5221 cagttatcga ctcacgccat tttacgacat catttcagca tttccggtcc ttggcggtac
     5281 gggaatacac atcagcgatc tcaaactggc aatggggctt aacgcatcca aaggcaaaaa
     5341 aacggcaatc gataaaattt atccgcgaca ttttttggcg acagcaaagg tgctgagatt
     5401 cccggaagtg cagatgcatg aaatcctaag tgactttgcc agaatgattc cggcagcact
     5461 ggataacgtg aagacttcat taccgacaga ttttccggag aacgtggtga cggcagttga
     5521 aaccaatgtg ttgaggttgc atggacggtt aagccgagaa tacggtagta agtgagatat
     5581 ggggtttggt cgttgttaat gagcacgaca atcatgaccg ccaacgactg gattcttcct
     5641 tggtttatac tgatgcgcat aatgtcaacg cgttatgttg aaaaggccgc tgcgaaaatc
     5701 gaatcccgca gcggcctctt tagcataacg tcattgtgcg aaccaatagc tactttttcg
     5761 tataactctc ttcgcattta gccaaaaaca gcagattagt agcggttaaa tgaagggtat
     5821 aac
//
LOCUS       DAMZII010000219         5735 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZII010000219 DAMZII010000000
VERSION     DAMZII010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.221"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            18..1007
                     /gene="ldhA"
                     /locus_tag="POA77_004603"
     CDS             18..1007
                     /gene="ldhA"
                     /locus_tag="POA77_004603"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6367929.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1118..1540
                     /gene="hslJ"
                     /locus_tag="POA77_004604"
     CDS             1118..1540
                     /gene="hslJ"
                     /locus_tag="POA77_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDI6367930.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1537..1803)
                     /locus_tag="POA77_004605"
     CDS             complement(1537..1803)
                     /locus_tag="POA77_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDI6367931.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2077..5601
                     /gene="nifJ"
                     /locus_tag="POA77_004606"
     CDS             2077..5601
                     /gene="nifJ"
                     /locus_tag="POA77_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDI6367932.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRYDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1431 a         1472 c         1486 g         1346 t
ORIGIN      
        1 tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt
       61 acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga
      121 cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg
      181 acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc
      241 gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag
      301 tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga
      361 cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg
      421 aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa
      481 tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc
      541 cgtatccaag tgcggcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt
      601 tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt
      661 tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg
      721 gtgcattgat tgattctcag gcggcaattg aagcgctgaa aaatcagaaa attggttcgt
      781 tgggtatgga cgtatatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg
      841 taattcagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc
      901 atcaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact
      961 taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc
     1021 ctgcattcca ggggagctta ttcagataat ccccaaagac ctttcatcct ctattcttaa
     1081 aatagccctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag ccgcgcttgt
     1141 tgcgctaagc ctgctgatgg cgggatgtgt aagtagtgac aaaattgctg taacgccaga
     1201 acagttacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga
     1261 taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa
     1321 ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat
     1381 gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat
     1441 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
     1501 acagacatta acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
     1561 ggcaagtgac tgttcactac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
     1621 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
     1681 actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac
     1741 aggctcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
     1801 catcttttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
     1861 gagggcgacg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
     1921 ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga
     1981 aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc gcagcaattt
     2041 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
     2101 gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc
     2161 agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttaaa gaacgtttgg
     2221 ggagacacac cacgcgtagt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat
     2281 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
     2341 atgatcccga cgctgtacaa actggcaggc gaactgacgc catttgtcct gcatgtagcg
     2401 gcacgtaccg ttgccacaca tgcactttct atttttggcg atcattccga cgttatggcg
     2461 gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg tccaggaagc gcaagatttt
     2521 gctctcattt cgcacatcgc aacgctgaag agccgcgtgc catttattca tttctttgat
     2581 ggtttccgca cgtcccacga aatcaataaa attgtcccgc tggccgatga cactattctt
     2641 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa cccggaacat
     2701 ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc
     2761 aacccatggt acgacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc
     2821 gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg
     2881 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
     2941 cgtggcgaaa aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac
     3001 ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa
     3061 cccggtgccc aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt
     3121 aataatggcg agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc
     3181 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg
     3241 aaagcgcgct ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg
     3301 gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat
     3361 ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
     3421 gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt
     3481 cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
     3541 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc
     3601 attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa
     3661 gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
     3721 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
     3781 ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
     3841 agttacagta gcaaaggcca ggatctggtg gaacgcaact ggcaggctct ggcgctggcg
     3901 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
     3961 ccagtggttt ccgatgccgc ccctgatttc gtgaaaaccg taaccgcagc gatgctcgca
     4021 gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc
     4081 accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc
     4141 tgtacccaat gtaaccactg cgttgccgct tgcccacact cagctattcg cgcaaaagtg
     4201 gtgccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg
     4261 cgtgatatgc gcgggcagaa atatgtcttg caggtagcac cggaagattg caccggttgt
     4321 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
     4381 aatatgatgt ctcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
     4441 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg
     4501 attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac gccgtatatt
     4561 aaattgctga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac cggctgttct
     4621 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
     4681 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
     4741 acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
     4801 ccggcggaat tactgacggc gttgaaatca gacgccacgc cagaggttcg tcgtgaacag
     4861 gttgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact gctacgtgat
     4921 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gtgatggctg ggcttacgat
     4981 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg
     5041 ctggatacgc aatgctattc caacaccggt ggtcaggcat cgaaagcgac accgctgggt
     5101 gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
     5161 atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag
     5221 acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat cattgcttat
     5281 agcccgtgtg aagagcatgg ttacgatctg gcactcagcc acgaccagat gcgccaactc
     5341 acagctaccg gcttctggcc gctatatcgc tatgatccgc gtcgtgccga tgaaggcaaa
     5401 ctgccgctgg ccttggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac
     5461 gagcaacgtt tccgtcggct gaattcgcag cagccagaag tggcagaaca gttatggaaa
     5521 gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg
     5581 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaaagc
     5641 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
     5701 ccaacagaaa ataaaatctg tgacaataaa ggcat
//
LOCUS       DAMZII010000220         5666 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZII010000220 DAMZII010000000
VERSION     DAMZII010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.222"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(296..1255)
                     /gene="nrdF"
                     /locus_tag="POA77_004607"
     CDS             complement(296..1255)
                     /gene="nrdF"
                     /locus_tag="POA77_004607"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI6367933.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1265..3409)
                     /gene="nrdE"
                     /locus_tag="POA77_004608"
     CDS             complement(1265..3409)
                     /gene="nrdE"
                     /locus_tag="POA77_004608"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI6367934.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3382..3792)
                     /gene="nrdI"
                     /locus_tag="POA77_004609"
     CDS             complement(3382..3792)
                     /gene="nrdI"
                     /locus_tag="POA77_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI6367935.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3789..4034)
                     /gene="nrdH"
                     /locus_tag="POA77_004610"
     CDS             complement(3789..4034)
                     /gene="nrdH"
                     /locus_tag="POA77_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI6367936.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4282..4611)
                     /locus_tag="POA77_004611"
     CDS             complement(4282..4611)
                     /locus_tag="POA77_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI6367937.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4763..5107
                     /locus_tag="POA77_004612"
     CDS             4763..5107
                     /locus_tag="POA77_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDI6367938.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5144..5593)
                     /gene="alaE"
                     /locus_tag="POA77_004613"
     CDS             complement(5144..5593)
                     /gene="alaE"
                     /locus_tag="POA77_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDI6367939.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1453 a         1397 c         1408 g         1408 t
ORIGIN      
        1 ttttttctac cctaacatac tgagaatctg aggcaacccc tgagggcaaa tgtggcatga
       61 atattgatgc gagcaaatcg ccggaatttc ccgtgatata agggctgaga gcaaatcgag
      121 aaaagttgat attttttgtc atagatagcg aaaaaacgcc tgaatattgt agtttcaggc
      181 gaataatgca acagagtatc agtgtagatc actacagaat atttgcgtga taaaaagcta
      241 tttggcggga attatttccc tgctgcgggt agtgatattt ttgaaaataa caccctcaga
      301 aattccagtc ttcatcttct gtttcaaccg ctttccccat cacataagag gagcctgaac
      361 cggaaaagaa atcgtgattt tcatcggcat tcggcgaaag cgcggcgagg attgccggat
      421 tcacttccgc catttctgcg ggaaataacg gttcgtagtc cagattcatc aaagccttat
      481 tggcgttgta acagagaaac gctttcacat cgtcagccca cggggtttcg gcgtacagct
      541 catcggtgta ttgcaactcg ttgtcgtaga gttccagcaa caaatcgaag gcgaaactct
      601 tcaactcttc acgttgtccc agagatatct tttccatgtt tttctgatat ttatagccta
      661 tgtagtaacc gtggactgct tcatcgcgga taatcagacg gatcagatct gctgtattgg
      721 tcagctttcc gcggctggaa aaatacatcg gcagccagaa accggaatag aacaaaaaag
      781 attcaagaaa cacactggcg attttctttt tcagcggatc atcaccgcga taatgttgct
      841 gaataatctg agcttttcgc tgcaacggtg cgttttcttc actccatgcg taggcggcat
      901 cgacatcttt ggtctgacac agcgtcgaga aaatcgaact gtaagagcgg gcatgaaccg
      961 cttccataaa gctgatattc gataataccg cttcttcatg aggcgtgagt gcatcgggca
     1021 tcagcgaagg cgcgccgata acattttgca gcgtgtcgag cagcgtcagg ccagtaaaaa
     1081 cgcgcatcgt cagttgttgt tctacgacag ttaatgtctg ccaggcagga atatcgttcg
     1141 acagcggcac cttttctggt agccagaaat tgctggtcag gcgattccac acctccagat
     1201 ctttatcgtc agatatcttg ttccagttga tggcgctgat acgtgagagt ttcatagata
     1261 ttccttaaag tgcacaggag acgcagcctt caatttcagt gccttccagc gccatctgac
     1321 gcaggcgaat gtaatagagc gttttgatac ctttgcgcca ggcgtaaatc tgcgctttgt
     1381 tgatatcgcg agtggtggcg gtatcgggga aaaacagcgt cagcgacaaa ccctgatcga
     1441 catggcgagt cgcttccgcg taggtgtcga tgatcttttc tgcgccgatt tcgtaagcat
     1501 cctgatacag cgccagattc tcgttagtca taaacggggc agggtagtaa acgcgtcctg
     1561 ttttgccctc tttgcgtatc tctactttcg ccacaatcgg atgaatactc gacgtagcat
     1621 ggttgatata agagatagaa ccggttggcg gcaccgcctg aagattctgg ttataaatgc
     1681 cgtagcgcat cacgtcgtcg cgcagttgcg cccacatctc acgggtaggt aacgtaatac
     1741 cgctacgggc aaacagttcg ccaactttcg tcgttttcgg ctgccagttc ccttgcagat
     1801 attggctgaa atattcacta ctggcatagc gtgactgttt gaacccggcg aaggtttcac
     1861 cgcgttcgcg tgccagcaac atcgaggtac gcagtgcgtg ccaggtgatg gtatagaaat
     1921 agagattggt gaaatccagt gcttccggcg aaccataagc gatgccttct cgcgccagat
     1981 aaccgtgtaa attcatctgc cccagcccga tggcgtgtga ggcggcattt ccggcttcga
     2041 tggacggcac gctgcggata tgactcatat ctgacaccgc cgttaaaccg cgtacggcag
     2101 tctctaccgt gcgggcaaag ttgggggaat ccatggtgtg ggcaatattc aacgaaccta
     2161 aattgcagga aatatcatgg cctgtacggg catagtcgag attctcgtca tactctgagg
     2221 cgctgttaac ctgcaaaatt tctgagcata gattactcat atttatgcgc ccggcgatag
     2281 ggttagcacg gtttaccgtg tcttcataca tgatgtaggg atagccggac tcaaactgga
     2341 tttctgccag tcgctggaag aaatcacggg cgttgaggta ttttttgcgg atgcgttcat
     2401 cggcaaccag ttcgtcatag tgttcgctaa tggcgatatc ggcaaacggc ttgccataaa
     2461 ctcgctcaac gtcatagggc gaaaacagcg ccatctgcgc attctctttg gccagatgga
     2521 aagtgatatc cgggatcacc acgccaagcg acagtgtttt aatgcggatt ttttcgtcgg
     2581 cattttcccg tttcgtgtcg agaaaacgca gaatatcggg atgatgagca tgtaaataga
     2641 ctgcaccagc cccctgacga gcgccgagtt ggttggcata ggaaaatgcg tcttccagca
     2701 ttttcatcac cggaattacg ccagaagatt gattttcaat acgtttaatt ggcgcgcccg
     2761 cttcccgcag attcgacagc aaaaatgcta cgccgccgcc gcgtttcgac agttgcagtg
     2821 cggaatttac cgcccgacca atcgactcca tattgtcttc aatacgcagc aaaaaacagg
     2881 aaaccagttc gccgcgctgc tgcttaccgc agttgaggaa tgtcggcgtg gctggctgaa
     2941 agcgtcctga cagcatctca tcggtcagtt gcaacgccag tgtttcatcg ccttgtgcca
     3001 gcgttagcgc caccatcgtg actcgatcgg caaaatcttc cagataacgc ttaccgtcga
     3061 atgtcttcaa cgtatagctg gtgtaaaact tccatgcccc gaggaatgtc tggaaacgaa
     3121 aaccgctggt gtgtgcgtgg gcaaacagcg taatgacaaa gtcgcgagag tagcgattaa
     3181 gaacgctttc atcatagtaa ccttcgttga ccagccagtt caggcgctgc tgctggctac
     3241 tgaaggtcac actgttcgga cgcacatgcg tcgccataaa ggcgtcaacg gcctggcggt
     3301 ctttatcgaa ctgaatgcga cctgcgctat cgtagaggtt aagcatcgca ttcagcgcgt
     3361 ggtaatccat cgtttcctgc gtcaggcatt ctgcggttgt cgttgccaaa attcggttac
     3421 tcctttacga acgttttcga tatcgctttg cgtacccatg agttcaaaac ggtacagcca
     3481 cggcacgccg catttctgag caatcacatc tccggcacgg ccatacgcct caccaaagtt
     3541 gcgattaccc gaagcaataa cgccgcgaag caacgcccgg ttgtgctcgt cgtttaaaaa
     3601 gcgaattacc tgtcgtggca ccgcgccagc cgtaccgccg ccgccgtaag agggcacgat
     3661 caggatgtaa ggctcgtcta cctgaatccg ttcccgctca ttgagcggga tgcgcaccgc
     3721 gggcagacct aaacgttcga taaaacgctg cgtattttcg gagctgctgg agaagtagac
     3781 gagctggctc atgcactggc cgcgtgtggc gctggatgca gacggttaat catgtccgga
     3841 cggaaaccag accagctaag atcgccagca atcactaccg gcaactgacg aaagccctga
     3901 gcacgcaacg cttctgccgc ttcaggaacg cgatcgacat taatcatttc aaaatcaaag
     3961 ccccggtttt ccatcgcccg tttggtggcg tggcactgaa cgcaatcgtt acgagtgtaa
     4021 atagtaatgc gcatgattcg tatttccgtt taaaatgaag atacggcgcg atgatacgcg
     4081 tcgggttgtc tcactgttga tacagagata ctagatgtag ttgaaaaaag attcaaccac
     4141 acaatatata gcaaatagtg gtcgaaatta ccctggatat gagcgtgacg gggtagggga
     4201 atttttgtga ttcaccaggc aaaaagaaac cccgaagaca ggcttcgggg tcaaagacgc
     4261 gtatttatta tcatttttgc actacgattt gcgcatgctt aacagtgcgc cgataaaaat
     4321 acctaccgca gctgctgtac ccacgctaca ccagggtctt tcacgaacaa aagaatccgc
     4381 gcagccaacg gcatcgcgtg cggcttgctg gacgcgagta cgaccatgca ttcgcgccct
     4441 ggtttctttc agcaatgcct gagctttact gcgtgcggct tcagcttccc ctttggcgtc
     4501 gctgccccag gactttaata cagattcgag gctgtcagct aattgattga catcattctg
     4561 gatatcctgc acgccatcat caacgtcgtt gcggttcggt cggttaaaca tatgatcccc
     4621 cattagaatt cgatgtaatt ttcagtgtag accataattt tcttaactga ggttcatcgc
     4681 ggctttatgg acttttctga aagcgttgtg aatgcgcaat caggtaaggt aggcccgcgg
     4741 ttgataataa cgaggatcaa atatgtattt acgaccagac gaggtggcgc gcgtacttga
     4801 aaaagtcggt tttactgtcg atgtggtaac ccagaaagcg tatggttacc gccgtgggga
     4861 aaattatgtc tatgttaatc gcgaagcgcg aatgggacgt accgctctgg taattcatcc
     4921 aacattaaaa gaacgcagtt caacactggc tgaacccgct tccgatatta aaacttgcga
     4981 tcattaccag caatttccgc tctatttagc aggcgagcga cacgagcatt acggtatccc
     5041 gcatggcttc agttcgcgtg ttgcgcttga acgttatttg aatggtttat ttggcgaagc
     5101 cagttaaaga cgcgactggc gatgccagtc gcgaaaagaa gagtcaggct tttacctgct
     5161 ggtaacggct gactttaaac agtcggcggc aataatcgag gaagtagcca taaaccgccc
     5221 ccatcaacat cgaaacaacg atgtttgaac tgaccgccgc cataatctga tgccagtctg
     5281 cgcccaccac taacaagatc gccacataca ccggtgactg gaacgtcaca taagccagga
     5341 tatcagccag attttttatc cagcccgacg ggctaacttt gcgtgccgcg cgcataaaca
     5401 gatcgcggta cataccgtat ggccatgcaa ttaagatgtt caccggaatc gctaccaatc
     5461 tggaataaaa agactgttcg aagctcattc cggagaggaa aacttcaata cacatgttca
     5521 cgacagaaca gtaaacaacc atcgcgaacg tatctgcaac tgcatgacgc aagcgtgact
     5581 gcggtgagaa catgctaatg ctccttaata aaagcgtaat gggatgttaa tggagatgaa
     5641 agcagtggat ctcattgtca tctgca
//
LOCUS       DAMZII010000221         5528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZII010000221 DAMZII010000000
VERSION     DAMZII010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.223"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="POA77_004614"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="POA77_004614"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDI6367940.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="POA77_004615"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="POA77_004615"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDI6367941.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /locus_tag="POA77_004616"
     CDS             complement(3139..3438)
                     /locus_tag="POA77_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDI6367942.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="POA77_004617"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="POA77_004617"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDI6367943.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..5087)
                     /locus_tag="POA77_004618"
     CDS             complement(4650..5087)
                     /locus_tag="POA77_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDI6367944.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGYYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5133..5474)
                     /locus_tag="POA77_004619"
     CDS             complement(5133..5474)
                     /locus_tag="POA77_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367945.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWVSCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1344 a         1427 c         1361 g         1396 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccaaaatg
      361 ttggagacac gcttgttcgc cgccgccagt gcagcagctg cttcaagggt acggaagtgc
      421 gataccgctt tcatgcgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aaccacgttg gcgttagtca gcttatcgcc atacagacgc
      601 actgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg
      721 ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct
      781 accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc
      841 accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcgcg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc
     1141 gccagacgtg gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaattttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct
     1741 tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc
     3361 tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa taaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata
     4861 taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac
     4921 gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc
     4981 atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag agctatccat
     5041 gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt
     5101 tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga
     5161 ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt
     5221 gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata
     5281 tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca
     5341 aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag
     5401 acccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt
     5461 tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt aaccaacgaa
     5521 agacaaaa
//
LOCUS       DAMZII010000222         5403 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZII010000222 DAMZII010000000
VERSION     DAMZII010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.224"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(114..1397)
                     /gene="aroA"
                     /locus_tag="POA77_004620"
     CDS             complement(114..1397)
                     /gene="aroA"
                     /locus_tag="POA77_004620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI6367946.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1468..2556)
                     /gene="serC"
                     /locus_tag="POA77_004621"
     CDS             complement(1468..2556)
                     /gene="serC"
                     /locus_tag="POA77_004621"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDI6367947.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2755..3447)
                     /locus_tag="POA77_004622"
     CDS             complement(2755..3447)
                     /locus_tag="POA77_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDI6367948.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3577..5337
                     /locus_tag="POA77_004623"
     CDS             3577..5337
                     /locus_tag="POA77_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDI6367949.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAISGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1391 a         1397 c         1276 g         1339 t
ORIGIN      
        1 gacaagcgac gctaccatta atcaaaatga ctcacaaggt ccgaaaaaaa acgccgacaa
       61 tgtgccgacg ttttgataaa gaaagatttg gctatttatt gcccgttgtt cattcaggct
      121 gcctggctaa tccgcgccaa ctgctcgaaa taatccggaa atgttttggc cgtacatttg
      181 ggatcaagaa tcgtcactgg tgtatctgac aacgccacca gcgagaaaca catcgccatc
      241 cggtgatcat tgtacgtcgc gatctcggca aagttcagtt tttccggtgg agtgatacga
      301 atgtaatcgt gcccctcttc tacttccgca ccgactttac gcagttctgt tgccatcgca
      361 aacaggcgat cggtttcttt aacacgccag ttatagatat tgcgcagcgt ggtggtgcct
      421 tttgcaaata acgccgccgt ggcaatggtc atcgccgcat cgggaatatg gttcatatcc
      481 atatcaatag cgttcagttc accacgcgtg caggaaatat aatcatcgcc ccagcaaatg
      541 gtcgcgccca ttttttccag cacatcagca aagcgaatat caccctgcat actgttacgt
      601 ccaataccgg tcacttttac agtgccgcct ttgattgctg ctgctgccag aaagtaagaa
      661 gccgaagatg catcgccttc gaccaaataa gtacccggag actgataaga ctgcccgcct
      721 tttacgacaa attgttgata gtgctgattt tcaatttcaa caccaaacgt cttcatcaga
      781 ttgagtgtga tgtcgatata aggtttagaa accagatcgc ctttaatacg aatcaccgta
      841 tcttccggcg caagaggcgc agtcattaac agtgcggtga ggaattggct ggaaacggag
      901 ccatcaacgt caacgttgcc gccagtaaag ccgccctgta aacgcaacgg cggataattt
      961 tcttgttcca ggtaagtgat cttcgccccg cccaggcgca gcgcatccac cagatgacca
     1021 atcgggcgtt ctttcatacg cggctcaccg gtcagcacaa tatcattgct acccagacaa
     1081 agagctgccg ccagcggacg cattgccgtt ccggcgttac cgaggaataa ctccagggca
     1141 ccttctgcgt gtaatggacc gccgttaccg ataatttcgc aacgcgtacg atcggctgaa
     1201 agcgtatagc ttacccctaa cgctgttaat gcattcagca tatggcgcac gtcatcgcta
     1261 tccagcagat tggttaatac tgttttgccg tgtgctaatg ccgccagcaa taaagcgcgg
     1321 ttagaaacgc tcttggaacc gggcagatta atagtgccat cgacacgagc gatgggttgt
     1381 aacgtcaggg attccatgaa ctcaactctc tacaacagaa ataaaaaccc cacaggctgg
     1441 ctgtggggat taagcaaaat ttcggcatta accgtgacgg cgttcgaact caaccatgaa
     1501 gtctgtcagc gctttaacgc cttccagcgg catggcgtta taaatagaag cgcgcattcc
     1561 gccgaccaca cggtgacctt tcagtgcatg aaggccagca gcaaaagact cttcaaggaa
     1621 caatttgtca agcgcactgt ccgccaactg gaacggcacg ttcatccgcg aacggttagc
     1681 tttcgccacg tcattgcggt agaaatcgct gttatcaatc accccatata gcagttctgc
     1741 tttttgctga ttgattttat ccatttcagc tacaccgccg ttcgctttca gccatttaaa
     1801 gaccagacca gatagatacc aggcaaatgt cggcggcgtg ttaaacatgg agccgttatc
     1861 gttgaggatg gaataatcca gaatcgacgg acacgcgata ttcgctttgc ccagcaaatc
     1921 ttcacgaacg atgacgattg tcaggccagc cgggccgata tttttctgcg cgccagcgta
     1981 aatcacgcca taacggctga cgtcaatcgg acgggaaaga atggttgaag agaagtcggc
     2041 ggcgaccacc acatctttgc cgaagtctgg cgtttcgtcg atggcgatac cgtcgatggt
     2101 ttcattcggg cagtaatgca tataagcagc gttatcagag agttgccatt cacgcattgg
     2161 cttaaccgcg cgcagaccat caacagtcac tttggcgtca aagacattag gcgtgcagta
     2221 ttttttcgct tctttaatgg cacttgccgc ccagtaaccg gcatcaacat aatctgcggt
     2281 ggttttatca ccgagaatat tcagcggtac cgcagcaaac tgaccgcgac caccgccatg
     2341 gcagaataat accttgtagt tggaggggac attaagaaga tcgcgaaaat ccttctcggc
     2401 ttcctctgca acctgaatga actctttgcc acggtgactc acttccatca ccgacgtacc
     2461 aagaccgttc cagtcgcgca gttcctgttg agcctgttta agcacctctg ccggtagcat
     2521 tgccggacca gaactaaaat tgaagatttg agccatttcc cctcaccacg ttgcgttgct
     2581 attaacccgc ggtcaatcga ttgcgaccac aggtaataca ttattcctgt ggctatcggt
     2641 tttatcattc agtgacacac atcgcaatga gtaaaaccgt tgagggcaaa aatgtggctt
     2701 gcatcacaca aaagaatctc tcggattatt agcaaaaagc cgatgttttt actgtcaggt
     2761 cacccgtttt gcccgacttg ccttcgtcca ttctggattt gcacagcgcg gacataattg
     2821 tttttccccc gctttcatat gaatgatttc actacaacga atacaggcat agtccgccga
     2881 ctccggcacc actttgtaac gctgcgtaca atcactgggt aaaataagta aacccggttt
     2941 caccttgtca tcttcaaaga aatagacact gatttgcccg ttggtttcga gaatcgccag
     3001 acgtacctgc cccagctgct ccacgccacg caatcgtagc tccataaaga actcaaattc
     3061 cgtcatgttg gagttattga gtttcgacca ggccagctcg ccatcttcaa taataacaac
     3121 cggcttacct tccagtaaat cttccagttt ttcactgtgc gccatcaacc acattaccag
     3181 gcggtataac aacgccagag tgataaagac gataagtacc ggcaccatcg gcacatcatc
     3241 ataaaacgcc acatctcccg ccgctgaacc cagcgtgaga atgattaaca cttcaaacag
     3301 cgacatctgt cgcacaccgc gtcttccggt cattttgagg aacagaaaaa ccagtacaaa
     3361 ggtataaaga ctacgtagtg caacttcgcc aaggaaatca aaaggcactt tatcaaatgc
     3421 catacggtgg agatcgaacg ccttcatttt tatgctctta tcgtggttta tccgctaaaa
     3481 gcatagtcaa cagttcatcg gcatggcggc ggtgatagcg cctttgagat aaaacccgta
     3541 ttattgcgcg ctttccgtac gactaaagtg attttcatga cgcaaacatt tatccccggc
     3601 aaagatgccg ctctggaaga ttccatcgct cgcttccagc aaaaactttc agacctcggc
     3661 tttcagattg aagaggcctc ctggctgaat cctgtgccta acgtctggtc tgtacatatt
     3721 cgcgacaaag agtgcgcact gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg
     3781 ctggcttctg cacttggtga atatttcgag cgtctctcaa ccaactactt ttttgctgat
     3841 ttctggctgg gcgaaaccat cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg
     3901 ttcccactga ccgaaaatga cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg
     3961 ttttatgatc cggagaatga actgaccggc agcatgctga ttgacctaca atccggtaac
     4021 gaagatcgtg gtatttgcgg cctgccgttt acgcgtcagt ccgacaatca gaccgtttat
     4081 attccgatga atatcattgg taacctgtac gtctccaacg gtatgtccgc aggtaatacc
     4141 cgcaacgaag cacgcgttca gggattgtct gaagttttcg aacgctacgt gaaaaaccgc
     4201 attattgctg aaagcatcag cctgccagag atcccggcag acgtgctggc gcgttaccca
     4261 gcagtagttg aagccatcga aacgctggaa gcggagggtt tcccaatctt cgcttatgac
     4321 ggttcgcttg gcggccagta tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt
     4381 acctgctttg cctctttcgg tgcgcatcct gattttggcg tagcactgga acgtaccgtg
     4441 accgagctgc tgcaaggtcg tggcctgaaa gatttggatg tgtttactcc gccaaccttc
     4501 gatgatgaag aagtcgctga acataccaac ctcgaaacgc actttatcga ttccagcggt
     4561 ttaatctcct gggacctgtt caagcaggat gccgattatc cgtttgtgga ctggaatttc
     4621 tccggcacca cggaagaaga gttcgccacg ctgatggcta tcttcaaaaa ggaagataaa
     4681 gaagtttata ttgccgatta cgagcatctg ggtgtctacg cttgccgcat tatcgtgcct
     4741 ggcatgtccg atatttatcc ggctgaagat ctgtggctcg cgaataacag tatgggcagc
     4801 catttacgtg aaacgattct ttcgctacca ggcagcgagt gggaaaaaga agattacctg
     4861 aacctcatcg agcaactgga tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg
     4921 ttgggtctgg cgaccgggtc ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa
     4981 gccatgctgg cgctggctgg tggcgatctg gaacaggctc tggtctggac cgaatggacg
     5041 atggagttta actcatcagt atttagtccg gaacgcgcca actattatcg ctgcctgcaa
     5101 acgttgttat tactggcaca ggaagaagat cgccagccgc tgcaatatct gaatgcgttt
     5161 gttcgcatgt acggcgcaga tgccgtagaa gccgccagtg cggcaattag cggcgaagcg
     5221 gcgttttacg gcctgcaacc ggtagatagc gatctgcacg cgtttgctgc acatcagtcg
     5281 ttgttgaagg cctacgaaaa gctgcagcgc gccaaagcag cattctgggc aaaataaaat
     5341 caaatagcct acgcaatgta ggcttaatga ttagtctgag ttatattacg gggcgttttt
     5401 tta
//
LOCUS       DAMZII010000223         5349 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZII010000223 DAMZII010000000
VERSION     DAMZII010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.225"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..340)
                     /locus_tag="POA77_004624"
                     /pseudo
     CDS             complement(1..340)
                     /locus_tag="POA77_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            396..758
                     /locus_tag="POA77_004625"
                     /pseudo
     CDS             396..758
                     /locus_tag="POA77_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            751..1167
                     /locus_tag="POA77_004626"
     CDS             751..1167
                     /locus_tag="POA77_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDI6367950.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLIELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1169..1682)
                     /locus_tag="POA77_004627"
                     /pseudo
     CDS             complement(1169..1682)
                     /locus_tag="POA77_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(1743..1847)
                     /locus_tag="POA77_004628"
                     /pseudo
     CDS             complement(1743..1847)
                     /locus_tag="POA77_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1849..2172)
                     /locus_tag="POA77_004629"
                     /pseudo
     CDS             complement(1849..2172)
                     /locus_tag="POA77_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2915..3892
                     /locus_tag="POA77_004630"
     CDS             2915..3892
                     /locus_tag="POA77_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6367951.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(4177..4917)
                     /locus_tag="POA77_004631"
     CDS             complement(4177..4917)
                     /locus_tag="POA77_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6367952.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(5038..5226)
                     /locus_tag="POA77_004632"
     CDS             complement(5038..5226)
                     /locus_tag="POA77_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDI6367953.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
BASE COUNT         1342 a         1305 c         1316 g         1386 t
ORIGIN      
        1 tagccccact gttcgtccat ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc
       61 gaggttaccg gctgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc
      121 ccataatgcg ggcagttgcc tggcatccaa cgccattcat ggccatatca atgattttct
      181 ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt
      241 gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
      301 tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc
      361 atcatacact aaatcagtaa gttggcagca tcaccaagga acagatggct gtacatgcta
      421 atacaagagt gagctcattc caggctgaca atatcattgt gcaccgtaat gaacctgact
      481 acctttcccg tcgcatttat aatgctgaac agcgtgagtc tatcattaat gttatcaatg
      541 aacggcagaa gttgcttatt aaacgagttt atgatttcat cagccgtttc accgactata
      601 cacatgtaat gtgtgttggt ggtggggctg aaattgtggc cgaggctgtg aagaacctga
      661 ctaaggttcc tgatgagcgc ttttatctga gctcatcccc gcagtttgat ttagtcatgg
      721 gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg aaaataaatc cagacgctgc
      781 tcatttgaac ttttcccgga tgaacggaca ggagataaga tagcagatga gctcattgca
      841 aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga tgttagttac tggtgcggcg
      901 tttgcagcta tagataaacg tcttcctttg ctgatatcgg agttactgac agaaaatacg
      961 actctggatg atattaataa ggtgatttcc agcgtaatac caggtgcttt ttcggttgaa
     1021 aagaaattga ttgaattact ggaaaaacaa tctggtctcc atacgtctgt ggattgcagt
     1081 actcccctta cagagcagag tctgtcgaga aatgacggtg aagaccagac cagaagaaac
     1141 gctgaaaata tgttcggaga tgactgaact aacccagcct ggccagggga atatctcgcc
     1201 atttagttgt ataacactga ctcaacattt cccgtttcat ttgccattca ggggcggttc
     1261 ctcgccccgc aaaccacact ttcccttttc ccgactggtt cagttcatcg agtgttttca
     1321 ttaacttttc actgttttta cggggctgga tttcatcaaa taatcccggc tgcgctatac
     1381 ccgatggtgt gaaatcagcc agcatgactc ctgccttcat atagcggtac ccttcacgcc
     1441 agacatggtt taaggctctg catgcggcgg caataatgtc ccggctgtcc tgtgtgggca
     1501 atgaaagctt ttccacagcg gcattgctgt aacagggttc agggggatgt ccgtacaaat
     1561 gtcgtcacct gccggcaata ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca
     1621 tactgaacaa cagcctggtg catggcatct ttgtctgtga ttcgttcacc aaaactgcga
     1681 ctatgtcaac gccacgatgt ttgaccgtta tttgccattt tcatgcccgc taaatacttt
     1741 cgttactgat gctgtccgct ttgcaccatt tcatcatcac tgattggcgt tgtgtttttg
     1801 gtcagcactc cagctttacg tttctctttt aaccggtaac tctctccttg ttctttttga
     1861 cggacgcatt ttacgtttgg gctggatgta gtaacgcagc atggaacgtc cgcccgtata
     1921 acccattgcc ttaatctccg cgaagataac ctcgctattc cagacattct ctgccaggcg
     1981 catgtcgatg taatccataa acggtttcag cttaaccatt ttgtggcggg tctttctggc
     2041 tggcggttca gggtatttga ggtagcgtct gaccgtccgt tcagagcaac caatctgagc
     2101 cgcaatatcg acaatgtacg cgccctgctg gcgcatttgc tttatcatgt aaaagtcctc
     2161 tctgctcagc atgttgatgt cctttctggt gtgagaacct taaggaaaca acatgttggg
     2221 tggagcggac aatccaaatg gtgaattacc gtcttatatc actggcgctg acacctgtag
     2281 tttgtataca caatccctgg cattgaaaca cacatatgaa gtgtacttcg gatatgaccg
     2341 tacgctcagg aagtacggca gattccgtca aaaattacct gtaaagacag acatcagaaa
     2401 aaacagtgct cgccgcagac cggtgaccgg gcgaagcccg cgaaccggcg aggaagcgca
     2461 atatatctgt agttacagtg agcaggtacg ccctgaagcc ttcataatcg gtagtgtatg
     2521 ttgtttttct ggaatgattt atccacatat ccggaggccc gatccagtaa acagatccat
     2581 gaatgatcaa taaaggatcc attaaagatc cccatatcgc tacaaacctt gtcactcatg
     2641 ggccgggacc acgttcacat aagctgtggc atgttatgga taaactgtaa caggttcatg
     2701 ataagctgta ttcagtaatg catatactga agtaagttaa tgacataaac tatggtcagt
     2761 acgccagact cagttgttaa atacagactg caggtttttc ttcagtcagt tgacggggtt
     2821 ctgacacacg attttctgtt tattctttta ctgtccacag gcaggaggct ttctggaaaa
     2881 cgaaaattca gacatcaaaa aactgttcgg cgaggtggat aagtcgtccg gtgagctggt
     2941 gacactgaca ccaaacaata acaacaccgt acaacctgtg gcgctgatgc gtctgggcgt
     3001 ttttgtaccg acccttaaat cactgaagaa cagtaaaaaa aatacactgt cacgtactga
     3061 tgccacggaa gagctgactc gtctttccct ggcccgtgct gagggattcg ataaggttga
     3121 gatcaccggc ccccgcctgg atatggataa cgatttcaag acctgggtgg ggatcattca
     3181 ttcctttgcc cgccataacg tgattggtga caaagttgaa ctgccttttg ttgagtttgc
     3241 aaaactgtgt ggtatacctt caagccagtc atcccgcagg ctgcgtgagc gcatcagccc
     3301 ttccctgaag cgcattgccg gtaccgtgat ctcgttttcc cgtaccgatg agaagcacac
     3361 ccgggaatac atcacccatc tggtacagtc agcctactac gatactgaac gggatattgt
     3421 gcagttacag gctgatcccc gcctttttga actgtaccag tttgacagaa aagtccttct
     3481 tcagcttaag gcgattaatg ccctgaagcg acgggagtcc gcccaggcac tttacacctt
     3541 tatagagagc ctgccccggg atccggcacc gatatcgctg gcgcggctgc gtgcacgcct
     3601 caatctgaag tctcctgtat tttcccagaa ccagacggtc agacgggcaa tggagcagtt
     3661 gcgcgagatt ggatatcttg attacacgga gatccagcgg gggcggacaa aattcttctg
     3721 tattcactac cggcgtcccc ggttaaaagc gccgaatgat gagagtaagg aaaatccgtt
     3781 gcaaccttca cctgcggaaa aagtcagtcc ggagatggcg gagaagcttg ccctgcttga
     3841 gaaactgggc atcacgctgg atgacctgga aaaactcttc aaatcccgct gaacataaac
     3901 tgtagtcagt gaagagcgct cctttactga ctacagctta tattaaccgg tgcagtgagt
     3961 ggtctgctca ctgcagttta tattctgttt cctgcagtgc tgtctgtagc tgagctgcca
     4021 tctgcctgtc ccttacgtaa gtgatcccgt aacctgatgc tgaggcattg ctcccttcat
     4081 aaaacatgac ttactcacta cagcttatat acatgctcca gcttatgtta tgtctgttct
     4141 gctgaccaca gcttatataa ggaagcgcgt atgccgtcac ctcaggggag gcagtgtacg
     4201 caggatctcc gcagcatccc gcccgtcacc ggtgaaaggc actgccagcg tggcagccat
     4261 atccagtgca aacactctgg tataaacctc catcgaacgt ggatccctgt gaccagccag
     4321 tgcctggatg actttccggg gctggcggtg atagagcatg tgcatgatat agctgtgccg
     4381 gaaggtgtgt ggtgtgaccg gaatcgaaaa gtgtaccccg tcagcttcgg cccgtctgac
     4441 agcctgcttc agccagttgc gcatggtttc gtcggtcacg gcccataatg gttcacgacg
     4501 acggggccgg gtggtgatca tccagctttc catctgcctg acatagctta tatccgtcag
     4561 cggaaccagg cggacttcat cttttggcgg gcgtccgcgt cgcgcacgca ctttttcgga
     4621 caggatccgc acaaacggtc ttactccatc caggtcaaat gattccggcg tcagcatccg
     4681 ggcttcgcca atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg
     4741 atccggcata tagaagagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc
     4801 ccccgtggaa gtggccattc ttctaaggga gagcgctgtt gcaaaatcca cccccggcgc
     4861 aatgggtaac agggaaacgc gttctggtga attctgcagg ggaatgacat tgttcatgag
     4921 ttatttttca cgtagcacac gcactgttta tggatacagg tattctgacc tgaacgcccg
     4981 gatccttcca cgacaataaa tcgcagcgcg cggatccttt tgtttgctta tcacgcatta
     5041 ttgcgggtaa aaccaaatac gtaaaggtat gttttgcagc agggatcccg tattaagcca
     5101 gtcgtggcgc gggctgaggg ctggtttgcc gtttttggga gaattggggt aatgccagga
     5161 caggcagaaa cggagaaaaa atcctgcaga cgcccttaaa agacaactga aaatttctga
     5221 ctccatattc ctctggtttc agactgctta atcacatcat caacctgata cataacaaac
     5281 agattctgtt cacagaatga acaattaagt aaatagttag attatgcgaa aaagttacag
     5341 atatttctc
//
LOCUS       DAMZII010000224         5328 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZII010000224 DAMZII010000000
VERSION     DAMZII010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.226"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            109..1176
                     /gene="pilL"
                     /locus_tag="POA77_004633"
     CDS             109..1176
                     /gene="pilL"
                     /locus_tag="POA77_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HDI6367954.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1176..1613
                     /gene="pilM"
                     /locus_tag="POA77_004634"
     CDS             1176..1613
                     /gene="pilM"
                     /locus_tag="POA77_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDI6367955.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1627..3309
                     /locus_tag="POA77_004635"
     CDS             1627..3309
                     /locus_tag="POA77_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDI6367956.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3302..4597
                     /gene="pilO2"
                     /locus_tag="POA77_004636"
     CDS             3302..4597
                     /gene="pilO2"
                     /locus_tag="POA77_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDI6367957.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4584..5036
                     /gene="pilP"
                     /locus_tag="POA77_004637"
     CDS             4584..5036
                     /gene="pilP"
                     /locus_tag="POA77_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDI6367958.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
BASE COUNT         1533 a         1373 c         1201 g         1221 t
ORIGIN      
        1 tggtctctcc cgcatttgca ttcggacgga atgacagtcg tcattcgtcc tctccagccg
       61 cctcacacca attaacaaac acgtatatac gaacacggag tctccgttat gaaaaaaaac
      121 tatctgacac ttatactgcc gtcattattg tccggctgtg ccggaatttc cggcagcaat
      181 aacgtggcac catcagatac gcctgctctg gtttttgttg acggacaaat cagtgagagt
      241 gctggcgttc tggcacttac gcaacaacgt ctttctccac ctaaaataac tccccccccg
      301 agagtcagcg tgccagagac agtaaaaaca gcacatccag tcagcgaatc ccgaccgtta
      361 ccttcaccaa caataagtca gaatttctcc cggttaatca ttacaggcaa gcctggtagc
      421 gcccctgtaa cactatcgcc gacggcccgt aaccggaccg tcagtcagtg gataaaaaca
      481 ttactaccgg aaggatggaa attcagacaa gagaatgcag ccacgcccaa gctgaatacc
      541 aaactggttt catggtctgc caatgatcag tggacccgtt ctctgaatcg gttgctggaa
      601 gaacaacacc tatggggcca tctggactgg gataataaaa cgttgaccgt cacaacatca
      661 gctgctgcac cggcacagga cacggcaacc actatctcgc cagcagccat ggtaaccaca
      721 tccacttccc ctgaatctcc aacaacagca aatagccaga acaaaccacg aaatccattc
      781 agaggaaaca gcgtatcttc gtcaaccccc gccgccaccc cgactccaac cggctccaca
      841 gtcaagtcta tcccgctcat gaccggaacg cctgttaaac cggtatctca gggtaaagag
      901 tggcgtgcgc cggcaggcac cacattaaga gagaacatta tcaagtgggc agaagaaaca
      961 aaatgcgaaa gcatggcatc aacgcactgg atggtcatat ggccactttc tgtaaccgat
     1021 taccgtctgg atgcgccttt aatgttccgg ggatcatttg aatcagccat ggagcaggta
     1081 tttgagttat atcggactgc gcaaaaacct ttatacgcac aggcttctcg catgcagtgc
     1141 atcattacag tttctgatac aagggatggt cgctaatggg atggctggtc atggccgtcg
     1201 gcctgaccat tctgattatc actggtagct accagaatca gaagatgtct gaaaccacaa
     1261 acgcccagca atatgcaagt gcgtctgtat gggcaagtca gatactgatg attgctaaca
     1321 gaatcaatga tatcagatac gtatcaggcc agcaggacgg agtaatttcc tcagataaac
     1381 tggctctccc tgtcactccg gactcccgaa ttaaacatca gttacaacag gggcgtctgt
     1441 gggtatggat gcctgaacaa ccaggtcttg ttgaaacttt gcgttcaaaa agtcgtggtt
     1501 ctgcactaat agggatcttc caaaacgggc aactcacctg gctatcagga actgcaaccg
     1561 gtctgacacc cccggcaggc attactgctg gttctgtcgt ctacgttaac taatctggat
     1621 atattcatga aaaaatcaca ccagcgttca atgaagctgg cggtgctccc ctgcatgatc
     1681 gccgtcgctc tttccatctc cggatgtact ttcagcgaaa tcaacaaaat gcagaaaaaa
     1741 gcacaggaag actcagcaca tgcacgggaa aaggtatcag ccctttcggc ccgtaaatcg
     1801 caggctctta cctggctcga taatcaatgg ataaaccctg ttccggtcgc tcaggtatca
     1861 agagagaaaa aacaaacagc tccggcctgc tacatcacgc aggcaagaaa aggagagatc
     1921 actctgcagg aactggggca acgtattact gccgtatgcg gcatccctgt gatcatcacg
     1981 cctgacgcag ccaattcaac tcttgaagga ggcgctaccc gccagatgac aggaacacta
     2041 ccagcaccag atgaaaatgg gcgtctaccg ttaagcagcc tgggcagcac aacaatgact
     2101 acctccactc agccattaac gctgaataac ctcatgtggc agggagatat caatggtctt
     2161 ctggatctga tggccagccg gagtggtctg tactggcgca tggataatgg tcggattgta
     2221 ttctatctga ctgaaaccag aacgtatcca cttcatatgc tgaacaccaa aaccagcagc
     2281 agttccagtg tcagctctgg ctcaacaagc acaatggggg caacaggagg ccaggataac
     2341 tcagcatccg gtgatgcaac gtcctctcag agcacaaccg ttggtcagga atacgatctg
     2401 tatgaagaca tccggaaaac tattgaagca atgctgacac cagaaaaagg ccgttactgg
     2461 ttatctgcat cgagctcaac gctgactgtc actgatactc cagctgtcca ggaagccgtc
     2521 gcacgatatg tggacgaaca aaacagtatt atgaaccgcc aggtagccct gaacgtacag
     2581 gttctgagcg tcagcaatac cagaaacgaa cagttcggtc tggactggaa ccttgtttat
     2641 aaatcgctac attccgccgg agcaacgttg aacaatgcaa gcggagattt tacaggcgct
     2701 acatctgcag gcgtatcaat tctggatacg gcaacaggga atgccgccaa attcagcggt
     2761 tccagtcttc tgattaaagc gctgagtgaa cagggcgatg tcagtgttgt gacttcacaa
     2821 gaaagcactg tcacaaacct gacgccggta cctatccaga tggcagatca gacggtttac
     2881 gtcgcccagt cagcaacaac aacgactacg gatgtaggag caacaacaac attaacgccg
     2941 ggcatgatca ccaccggatt caatatgacc ctgctgcctt taattcagaa aacgggcaat
     3001 ctccagttgc agatgaattt taatctgtca gatcccccaa caatccgtag ctttacgtca
     3061 aaagacggaa acagttacat cgaaatgccg tataccaaac tgcgttcact gagccagaag
     3121 gtcaatctga aagaagggca atcacttgtc gttactggtt tcgatcagaa caatacgacg
     3181 acaagtaaag ccggtacgtt tacgccagca aatccattat ttggtggttc acaaaccggg
     3241 aaaaatgaac gcagcacgct tgtaatcatc attaccccga ctttcccgtc aggaggcaac
     3301 aatggctgat gaagatatca acccggttgt acttctcgca gatcctaaag taaatcatcg
     3361 cgtgtgggca gcctgtctta aatggagtcc tgtcgtaaaa aaacagcgag tcccttctca
     3421 tcagaaacac aagccccatg taaaatccag acggcttacc tctctgaaag tgactgtagg
     3481 cagcagaagt tcccgtggga agatcagccg cattaccggc acaggaatac tggccaggcc
     3541 ggagcgcaat cattattttt cccttgctct ggctttctgc tcatgggtga gaaatggata
     3601 cggcgtcttc cggtacagcg ataaggagct tttattcctt gcctccatta atggccagcc
     3661 tgccgttatg gctgatctgt caggaaatga tgctgatgtt gctcaaaagg tcagtctttt
     3721 tctgacgatg aacgaagagc cccccgaaaa atggcaggtc gtatctcctc tggaacatcc
     3781 tgataactgg gaaagtatta ttacccggtt atcatcagca gatctccgcc gttgcaaact
     3841 tactgtcggc aatcgcagca aattcacact gcctgccgta ctttttttag tagcagccag
     3901 cgcaggcact gtattctgga tgactcagcc agagccggat gtcgtaccga cgcccgagga
     3961 gatagctgca cgggcaagat tgcagtttaa aaagcccgat ccaccgccag aactcccgca
     4021 tccatgggca tcacaacctg taatcagtga ttttctgaaa gcctgcgcgg accttcgaaa
     4081 accatcaccg gtcgcgctag aaggctggaa acttaccggt ggaacatgta ctcccgagac
     4141 attcacactg atttacgaac gacagccagg aggaactatt gaaggctttc tggcaagaag
     4201 caaagaaatc tttaatgtta ttcctgactt taaccttaaa gatggtgcac gcctggcttc
     4261 tgtaacccgg ccactgcctt ccctgccccg cagggatgag gcagtcccta ccccctcaga
     4321 acaactgatg cgggtgttca cgtggtttca gaaaaaacag ttaacgccgg ccattaatga
     4381 aatagccata ccggaaccat taccaggtaa cgatggtgaa ccagcccctg ttcagaaatg
     4441 gaaagaatat cagttcagtt tatcaacgcc tgtaaatcct gatgagttgt tccctttatt
     4501 tcaggatacc ggcgttcgtc tgagcaatat ccattttgaa ttaaacggcg gtactttcag
     4561 ttattcctca gaaggtcata tttatgcgtc caggtaaatt actcattatt ccatccatat
     4621 ttttcttctc cggtttttca tttgcaacaa cacaacctct tgtcacaata ggagaacttg
     4681 aagcacaaca aaaccggaat attcttttac aggcaaaagt tcagggcgca cagctacaga
     4741 aacaactcga agaatcagat gttacttcat catctgaaac tgtttcaggt ttctctggtg
     4801 tgactgcctc tttgccatca gtttcagagc agccgacatc taaaaacaga aaggaattgc
     4861 ccgttattat ggaaataaac ggcaaagata aacgtcttaa tgccgttctt cgcatggctg
     4921 atggacgtca gacaagcgtc accactggca gccagcttcc aggcacatcc gtcacagtga
     4981 agtcgatctc attgtctggc gtcacgttaa gtgatggcac cacccttact ttctgagtaa
     5041 cgtgatatgg ataaatataa tctgaaggat gctctccttt ttatatccag gggagacacc
     5101 catgaaattc ttatagaaac aaaccagcgg acaaggcctg acgtacaaag taatctccag
     5161 gagctgctaa aattatatcc ggatatcaac ccaaaagtag tctcgttgtc ggagttacag
     5221 gaggcaggtc aggatgagaa aaacagaggc ccgaaagaac gaattattca tctgaaagac
     5281 cttgctgatg tcagtgaaag tgagaagaaa gttctatctt attttgaa
//
LOCUS       DAMZII010000225         5276 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZII010000225 DAMZII010000000
VERSION     DAMZII010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.227"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..307)
                     /locus_tag="POA77_004638"
     CDS             complement(<1..307)
                     /locus_tag="POA77_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6367959.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     "
     gene            379..729
                     /locus_tag="POA77_004639"
     CDS             379..729
                     /locus_tag="POA77_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HDI6367960.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            743..1018
                     /gene="merP"
                     /locus_tag="POA77_004640"
     CDS             743..1018
                     /gene="merP"
                     /locus_tag="POA77_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HDI6367961.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1054..1476
                     /gene="merC"
                     /locus_tag="POA77_004641"
     CDS             1054..1476
                     /gene="merC"
                     /locus_tag="POA77_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HDI6367962.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1528..3222
                     /locus_tag="POA77_004642"
     CDS             1528..3222
                     /locus_tag="POA77_004642"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HDI6367963.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3240..3602
                     /gene="merD"
                     /locus_tag="POA77_004643"
     CDS             3240..3602
                     /gene="merD"
                     /locus_tag="POA77_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDI6367964.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3599..3835
                     /gene="merE"
                     /locus_tag="POA77_004644"
     CDS             3599..3835
                     /gene="merE"
                     /locus_tag="POA77_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDI6367965.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            3871..4539
                     /locus_tag="POA77_004645"
     CDS             3871..4539
                     /locus_tag="POA77_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6367966.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            4614..>5276
                     /locus_tag="POA77_004646"
     CDS             4614..>5276
                     /locus_tag="POA77_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6367967.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVV"
BASE COUNT          929 a         1695 c         1694 g          958 t
ORIGIN      
        1 agtcggccat cttctcgcgc acgtccttga gcttgtgttc ggccaggctg ctggcctcct
       61 cgcagtgggt gccatcgtcg agccgcaaca gctcggcaat ctcgtccaga ctgaacccca
      121 gccgctgtgc cgatttcacg aatttcaccc gaaccacgtc cgcctcccca tagcggcgga
      181 tgctgccgta aggcttgtcc ggttcccgca acaggccctt gcgctgatag aagcggattg
      241 tctccacgtt gaccccggcc gccttggcaa aaacgccaat ggtcaggttt tccaaattat
      301 tttccatatc gcttgactcc gtacatgagt acggaagtaa ggttacgcta tccaatccaa
      361 attcaaaagg gccaacgtat gtctgaacca caaaacgggc gcggtgcgct cttcgccggc
      421 gggctggccg ccattcttgc atcgacctgc tgcctggggc cgctagtact ggtcgccctg
      481 ggcttctccg gtgcttggat cggcaacctg acggtgctgg aaccctatcg accgttgttc
      541 atcggcgcgg cgctagtggc gctgttcttc gcctggaagc ggatttaccg gcccgtgcag
      601 gcatgcaagc caggtgaggt ctgcgcgatt ccgcaggtgc gcgccaccta caagctgatt
      661 ttctggatcg tggccgtgct ggtcctggtc gcgcttggat ttccctatgt cgttccattt
      721 ttctattaac caggagttca tcatgaagaa actgtttgcc tcccttgccc tcgccgccgc
      781 tgttgccccg gtgtgggccg ctacccagac cgtcacgcta gcggttcccg gcatgacttg
      841 cgccgcctgc ccgatcacag tcaagaaagc gctctccaag gtcgaaggcg tgagcaaggt
      901 cgatgtgggc ttcgagaagc gcgaggccgt cgtcactttt gacgacacca aggccagcgt
      961 acagaagctg accaaggcca ccgcagacgc cggctatccg tccagcgtca agcagtgagc
     1021 cagcaagcca acgacaacag cgagagccgc ttcatgggac tgatgacacg cattgccgat
     1081 aaaaccggcg cgctcggcag cgtcgtttcc gcgatgggct gcgccgcctg ctttccagcc
     1141 ctcgccagct tcggcgcggc catcgggctg ggcttcttga gccagtacga gggactgttc
     1201 atcagccgcc tgctgccgct gtttgccgcg ctggccttcc tggcgaacgc gctgggttgg
     1261 ttcagtcatc ggcaatggct gcgcagtctg ctcggcatga tcggcccggc catcgtgttt
     1321 gcggccacgg tctggctgct cggcaactgg tggacggcga acctgatgta cgtcggcctg
     1381 gccttgatga ttggggtgtc gatctgggac ttcgtgtcgc cggcgcatcg ccgttgcgga
     1441 ccggacggct gcgaactccc cgccaagcgc ttgtgaaaga cggctgaccg tgcgacacgg
     1501 cggcccacac gaataaggaa cgatggtatg agcactctca aaatcaccgg catgacttgc
     1561 gactcgtgcg cagtgcatgt caaggacgcc ctggagaaag tgcccggcgt gcaatcagcg
     1621 gatgtctcct acgccaaggg cagcgccaag ctcgccattg aggtcggcac gtcacccgac
     1681 gcgctgacgg ccgctgtagc tggactcggt tatcgggcca cgctggccga tgccccctca
     1741 gtttcgacgc cgggcggatt gctcgacaag atgcgcgatc tgctgggcag aaacgacaag
     1801 acgggtagca gcggcgcatt gcatatcgcc gtcatcggca gcggcggggc cgcgatggca
     1861 gcggcgctga aggccgtcga gcaaggcgca cgtgtcacgc tgatcgagcg cggcaccatc
     1921 ggcggcacct gcgtcaatgt cggttgtgtg ccgtccaaga tcatgatccg cgccgcccat
     1981 atcgcccatc tgcgccggga aagcccgttc gatggcggca tcgccgctac cacgccgacc
     2041 atccagcgca cggcgctgct ggcccagcag caggcccgcg tcgatgaact gcgccacgcc
     2101 aagtacgaag gcatcttgga gggcaatccg gcgatcactg tgctgcacgg ctccgcccgc
     2161 tttaaggaca atcgcaacct gatcgtgcaa ctcaacgacg gcggcgagcg cgtggtggca
     2221 ttcgaccgct gcctgatcgc caccggcgcg agcccggccg tgccgccgat tcccggcctg
     2281 aaagacactc cgtactggac ttccactgaa gcgctggtca gcgagacgat tcctaagcgc
     2341 ctggccgtga ttggctcatc agtggtggcg ctggagctgg cgcaggcgtt cgcccgactc
     2401 ggagcgaagg tgacgatcct ggctcgcagc acgctgttct tccgcgaaga cccagctata
     2461 ggcgaagccg tcacggccgc attccgcatg gagggcatcg aggtgaggga acacacccag
     2521 gccagccagg tcgcgtatat caatggtgaa ggggacggcg aattcgtgct caccacggcg
     2581 cacggcgaac tgcgcgccga caagctgctg gtcgccaccg gccgcgcgcc caacacacgc
     2641 aagctggcac tggatgcgac gggcgtcacg ctcaccccgc aaggcgctat cgtcatcgac
     2701 cccggcatgc gtacaagcgt ggaacacatc tacgccgcag gcgactgcac cgaccagccg
     2761 cagttcgtct atgtggcggc agcggccggc actcgcgccg cgatcaacat gaccggcggt
     2821 gacgcggccc tgaacctgac cgcgatgccg gccgtggtgt tcaccgaccc gcaagtggcg
     2881 accgtaggct acagcgaggc ggaagcgcac catgacggca tcaaaactga tagtcgcacg
     2941 ctaacgctgg acaacgtgcc gcgcgcgctc gccaacttcg acacgcgcgg cttcatcaaa
     3001 ctggtggttg aagaaggcag cggacgactg atcggcgtgc aggcagtggc cccggaagcg
     3061 ggcgaactga tccagacggc cgcactggcg attcgcaacc ggatgacggt gcaggaactg
     3121 gccgaccagt tgttccccta cctgacgatg gtcgaagggt tgaagctcgc ggcgcagacc
     3181 ttcaacaagg atgtgaagca gctttcctgc tgcgccgggt gaggacaagg aggtgtgcga
     3241 tgagcgccta cacggtatcg caactggccc ataacgctgg ggtgagcgta catatcgtgc
     3301 gcgactacct ggtgcgcggc ttgttacggc cggtggcctg caccacgggc ggctacggcg
     3361 tgttcgacga tgcggccttg caacggctgt gcttcgtgcg cgcggccttc gaggcgggta
     3421 tcggcctgga tgccctggcg cggctgtgcc gtgcgctcga cgcagcggac ggcgcacaag
     3481 ccgcagcgca gcttgccgtg ctgcgccagt tggtcgagcg gcggcgcgcg gcgttggccc
     3541 atctggacgc gcaactggcc tccatgccag ccgagcgggc gcacgaggag gcattgccgt
     3601 gaacgcccct gacaaactgc cgcccgagac gcgccaaccc gtttccggct acctgtgggg
     3661 tgcgctggcc gtgttgacct gcccctgcca tctgccgatt ctcgccgccg tgctggccgg
     3721 gacgaccgcc ggtgccttcc ttggcgagca ttggggtgtt gccgcgctcg cgctgaccgg
     3781 cttgttcgtt ctggccgtaa cgcggctgct gcgcgccttc cggggcggat catgacgagt
     3841 tcgcagcccg ccggatggac ggcggccgag ttggcgcagg cggcggcgcg cggacagctt
     3901 gacctgcatt accagccgct ggtcgatctg cgcgatcacc ggatcgctgg cgcggaagcg
     3961 ttgatgcgct ggcggcatcc gaggcttggc ctgttgccgc ccggccagtt cctgccgctg
     4021 gccgagtcgt tcggcctgat gccggaaata ggcgcgtggg tgctgggcga ggcctgtcgc
     4081 cagatgcaca agtggcaagg accggcatgg caaccgttcc gtcttgccat caatgtgtcc
     4141 gccagccagg ttgggccaac gttcgacgac gaggtaaagc gggtgctggc cgatatggcc
     4201 ctgcccgccg agcttctgga gatcgaactg accgaatcgg tcgcattcgg caatccagcc
     4261 ctgttcgcca gtttcgacgc cttgcgcgcc atcggcgtgc gcttcgccgc cgacgacttc
     4321 ggcaccggct attcctgcct gcaacatctg aaatgctgcc ccatcaccac attgaaaatc
     4381 gaccaatcct ttgtcgccag gctcccggat gatgcccgtg accaaactat cgtgcgggcg
     4441 gtgatccagc tcgcgcacgg gctgggcatg gatgtcattt tcagaagacg actgcaccag
     4501 ttgattgggc gtaatggctg ttgtgcagcc agctcctgac agttcaatat cagaagtgat
     4561 ctgcaccaat ctcgactatg ctcaatactc gtgtgcacca aagcgaggtg agcatggcga
     4621 cggacacccc acggattcca gaacaaggcg tggccactct gcctgatgag gcttgggagc
     4681 gtgcgcgccg tcgtgcggag atcatcagtc cgttggcgca gtcggagacg gtcgggcacg
     4741 aagcggccga tatggcggct caggcgctgg gcttgtctcg gcgccaggta tacgttctga
     4801 tccggcgtgc ccggcaaggc agcggcctcg tgacggatct ggtgcccggc cagtccggtg
     4861 gaggtaaagg taaggggcgc ttgccggaac cggtcgagcg cgtcatccac gagctactgc
     4921 aaaagcggtt cctgaccaag cagaagcgca gcctagcggc ctttcaccgc gaagtcactc
     4981 aggtgtgcaa ggctcaaaaa ctgcgagtgc cggcgcgcaa taccgtggcc ttacggatcg
     5041 ctagccttga cccgcgcaag gtcatccgcc ggcgggaagg ccaggatgcc gctcgtgacc
     5101 tacaaggtgt gggcggcgag cctcctgccg tgaccgcgcc gctggagcag gtgcagatag
     5161 accatacggt catcgacctg atcgtggtcg atgaccgcga ccggcaacct attggccgcc
     5221 cgtacctgac cctcgccatc gacgtgttca cccgctgcgt gctcggcatg gtcgtc
//
LOCUS       DAMZII010000226         5117 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZII010000226 DAMZII010000000
VERSION     DAMZII010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.228"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(40..498)
                     /locus_tag="POA77_004647"
     CDS             complement(40..498)
                     /locus_tag="POA77_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI6367968.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            618..1427
                     /gene="proC"
                     /locus_tag="POA77_004648"
     CDS             618..1427
                     /gene="proC"
                     /locus_tag="POA77_004648"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI6367969.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1444..2559)
                     /gene="adrA"
                     /locus_tag="POA77_004649"
                     /gene_synonym="dgcC"
     CDS             complement(1444..2559)
                     /gene="adrA"
                     /locus_tag="POA77_004649"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDI6367970.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2661..2981)
                     /gene="psiF"
                     /locus_tag="POA77_004650"
     CDS             complement(2661..2981)
                     /gene="psiF"
                     /locus_tag="POA77_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDI6367971.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3100..4515)
                     /gene="phoA"
                     /locus_tag="POA77_004651"
     CDS             complement(3100..4515)
                     /gene="phoA"
                     /locus_tag="POA77_004651"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI6367972.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4616..4876)
                     /gene="iraP"
                     /locus_tag="POA77_004652"
     CDS             complement(4616..4876)
                     /gene="iraP"
                     /locus_tag="POA77_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDI6367973.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1220 a         1365 c         1235 g         1297 t
ORIGIN      
        1 ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac tacgttgcac
       61 ttccagccac cacttctcca gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct
      121 atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt
      181 caggcgctca cgaatggtcg ctggcgtgta acgttcgccg cgcggattaa gcgccgctgc
      241 gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc
      301 ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt
      361 acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat
      421 ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac acgcgtcggc
      481 atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt
      541 ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc
      601 acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa
      661 aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat
      721 acaccccctc cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag
      781 aatcggcgca agaagtggcg caaatcgccg acatcatttt tgccgccgtt aaacctggca
      841 tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt
      901 ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa
      961 ttattcgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc tccgtaacgc
     1021 caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg
     1081 gcgaagcgga agtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt
     1141 cgccagccta cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga
     1201 tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg
     1261 tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca
     1321 ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgccgca gtgatcgaag
     1381 cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg
     1441 acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt aaagcgccaa
     1501 atctgccgat ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc
     1561 cacactaatc cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc
     1621 acgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc
     1681 aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa tttgtaactg
     1741 tcgggtaagc gccacaatcg cttcatcgcc cacatcatga ccccaggtat cgttgatgct
     1801 cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt
     1861 atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc
     1921 gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg
     1981 gtagctgacc cagccaaaca gcagaggata aacgacaata atgggaaggg agagccacca
     2041 ttccagcggc gcactattga acgacacggt aatgcccgta agctcgaggg tgacaaggca
     2101 ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa
     2161 attcagacac ataatcatca acatagcggt ggaaggcagc acgtttacgc ccattacgcc
     2221 tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg
     2281 atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac
     2341 caacaccagc caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa
     2401 gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga agcgcagccc
     2461 ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccatgcg ccgccgcttt
     2521 tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat tttcccaaat
     2581 tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccactt tcgtagctca
     2641 cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcactcat aaacttatta
     2701 cgatcatcac ctttcagaga ttgttgcgtt gcttgattat tacattcgcg catcttttgc
     2761 tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag gcaatcactc
     2821 atgtaggtct tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg attacaggag
     2881 gtcatacgct gttgttgtgg ggttaaggtt ttctcggcag cgccgacggt ggttaaaaaa
     2941 accagaccaa aaagcaaggt aaccagtaat gttattttca tagcaccatc cctcttcatg
     3001 ttttaaccat gagcgtttgc gcccgtgatc tgccattaag tctggttgct aacagcaaaa
     3061 aaaccacccg gcagcgaaaa ttcactgccg ggcgcggttt tatttcagcc ccagagcggc
     3121 tttcatggtg tagaagagat cggtctggtc ggtcagtcca acaacattgg cggcatgcgg
     3181 gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct cttcagagtt
     3241 cccgtaactc atcaccatca ctgcgccatc tttagtattt agcgcctgag tgaggcccgg
     3301 cgctttggta tccggcgcaa caacctggct ggcgtgggcg tgatcagcgg tgactatgac
     3361 cagcgtgtta ccctcctttt tagcgaattc cagcgcccgt tgtacggctt catcgagatc
     3421 gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat cgattgacgc
     3481 accttcaact tgcaggaaaa atcctttctc atttttactc aacaattcaa tggctttgtc
     3541 ggtcatctgc gccagggttg gtacactgtc attacgttgc ggattaggcg tacaggtgac
     3601 tgcgggctta tcgatattgc cgtggtacgt tgctttcggt cctagccagc gcactggcat
     3661 attgccgtca gcaaacagtc caagcagagg tttttgctga ttcgcttccg tcaccgcatt
     3721 tagcgaggcg gcatcgctga ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt
     3781 ttttccctgc cattcaccgg cggttgccgt ttcagcaaag gtttttgcgc caccgccaag
     3841 cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc gatccttttc cgcctttttc
     3901 cagagcgtta cccggacatt tttcactggt cgcgctcgga ccgtagcatt tgcgcgaagt
     3961 cacatgtgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag aaacgttacc
     4021 ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat
     4081 atcgacgccc agcgcgccgt tataggtttt gacaccggtt gaccaggcgg ttgctgatgc
     4141 agccgagtcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat agtgagtgta
     4201 ttgcccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac cttcggcata
     4261 attacgtgcg gcagtaattt ccgaatcccc catcccatcg ccaatcagca aaataatatt
     4321 ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcac ccgttaagcg
     4381 gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat
     4441 ttctggtgtc cgggcttttg tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat
     4501 agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata aaaacaaagc
     4561 gactataagt ctcggccgtg acaactttat gacagctgtt gaaaagatta actttttact
     4621 gacgaggatg cttcaataac ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag
     4681 gaatgctggc atcgggcttt acctcgtaca gcgccccctc tacctgatca atcaagcgct
     4741 gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac gataatctcc aaagcttcta
     4801 cctgcgcaca cagttctttc gactcttctt ctttttgggc aagcttaaat aacaactcag
     4861 caatgagatt tttcatgtct gtatttcctt atccaaagta tggagaagtt aacattatgt
     4921 ttcacaactt catagtgtct ggaagtatta tttttatgac aaaggaaata ctttgcgcaa
     4981 atattttttg tttgattacc agcgaaacgt ttctcttatt taaataatta cccacacaaa
     5041 atataggctt ttaattatca acctttatta taaataaaag ttatgaaata tattctcttc
     5101 gttaacttat aatttag
//
LOCUS       DAMZII010000227         5011 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZII010000227 DAMZII010000000
VERSION     DAMZII010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.229"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1900
                     /gene="mngA"
                     /locus_tag="POA77_004653"
     CDS             <1..1900
                     /gene="mngA"
                     /locus_tag="POA77_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDI6367974.1"
                     /translation="THRDALCLNARFTSREEAIHALTQRLAALGKISSTEQFLEEVYR
                     RESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVVLLAIPP
                     NEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASFSNAPTI
                     VCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNSATACIF
                     AAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDTQPVKSV
                     KTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMYRKLGGG
                     LLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAGYLMRWV
                     KNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNGLSGSNA
                     LLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFTVTASTM
                     LAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTGAIVGAM
                     NIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRHAVKHGN
                     YLTDGVMP"
     gene            1918..4551
                     /gene="mngB"
                     /locus_tag="POA77_004654"
     CDS             1918..4551
                     /gene="mngB"
                     /locus_tag="POA77_004654"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDI6367975.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVWQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4576..4803
                     /locus_tag="POA77_004655"
     CDS             4576..4803
                     /locus_tag="POA77_004655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367976.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
BASE COUNT         1240 a         1168 c         1359 g         1244 t
ORIGIN      
        1 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
       61 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
      121 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
      181 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
      241 gcttcagtgg gaaggcgttg atggcccgga agcggttgat ttagtggtgc tgctggcgat
      301 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
      361 ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
      421 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
      481 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
      541 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
      601 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
      661 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
      721 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
      781 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
      841 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
      901 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
      961 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
     1021 ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg cgttagcgcc
     1081 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
     1141 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag
     1201 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
     1261 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcctgg atcaataact cgcttaccgc
     1321 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
     1381 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
     1441 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
     1501 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
     1561 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
     1621 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
     1681 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
     1741 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
     1801 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
     1861 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
     1921 aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc
     1981 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
     2041 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
     2101 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggcaag
     2161 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
     2221 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
     2281 ggttatttac cagattcctt tggcatgtcc gggcaactgc cgcatatcta caatggattt
     2341 ggcattaccc gcaccatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc
     2401 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc
     2461 tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt
     2521 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatgggcat
     2581 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
     2641 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
     2701 cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat
     2761 cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag
     2821 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
     2881 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
     2941 ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa
     3001 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
     3061 agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt
     3121 aacaccactg tgcggctgcg cgccagccag tttaatctac gagacgatcg cggtcagcct
     3181 gtaccgtatt ttattcgcca tgcccgtgag atcgatccag gcctaatcga tcggcaaata
     3241 gttcattacg gtaattacga tccctttatg gagtttgata tacagatcaa ccagattgtc
     3301 ccttctatgg gctatcgcac gctttatatc gaagcgaatc agcctggcaa cgtgattgcg
     3361 gcaaaaagtg acgctgaagg gatactggaa aatgctttct ggcaaattgc gctcaatgag
     3421 gatggaacgc tacaactggt agataaagat agcggtgtgc gctatgaccg ggtgttgcaa
     3481 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
     3541 gtaatgactt ctgcaacagc gaaaccgcaa tgcgagatta ctcatgaggc ctggcagagc
     3601 agggctgtta tccgctatga catggcggtg ccgctcaatt tgttggaacg aagcgtctgg
     3661 caatccacgg gcagagtagg ggtggagatg gttgtcactc tgagtcataa cagcagacgt
     3721 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg cgtcctgatc
     3781 cctacatctt ttaacaccga tagtgttctg gcagataccc agtttggttc gctaacgcgc
     3841 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
     3901 ccggtatgga atatgctcaa ctatgcagcc ttacaggaag ggcgtaacgg catggctgtc
     3961 tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
     4021 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgtttttaag gcctgggcgg
     4081 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
     4141 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
     4201 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
     4261 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
     4321 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
     4381 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
     4441 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
     4501 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
     4561 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
     4621 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
     4681 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
     4741 ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt aattataata
     4801 taattataag ttaattaaat gttaatatca gcggggtgga tttatgcctt tattagtaat
     4861 cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc aaccgtgctg
     4921 tttttgcttc gtctcttttt atctttaatt gccaaccgaa actaatttca gccttataac
     4981 tcacacattt taaacataaa tgtcactaaa g
//
LOCUS       DAMZII010000228         4941 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZII010000228 DAMZII010000000
VERSION     DAMZII010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.230"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            102..1436
                     /gene="garP"
                     /locus_tag="POA77_004656"
     CDS             102..1436
                     /gene="garP"
                     /locus_tag="POA77_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDI6367977.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1452..2222
                     /gene="garL"
                     /locus_tag="POA77_004657"
     CDS             1452..2222
                     /gene="garL"
                     /locus_tag="POA77_004657"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDI6367978.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2252..3142
                     /gene="garR"
                     /locus_tag="POA77_004658"
     CDS             2252..3142
                     /gene="garR"
                     /locus_tag="POA77_004658"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI6367979.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3239..4384
                     /gene="garK"
                     /locus_tag="POA77_004659"
     CDS             3239..4384
                     /gene="garK"
                     /locus_tag="POA77_004659"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDI6367980.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4417..4793
                     /gene="rnpB"
                     /locus_tag="POA77_004660"
     ncRNA           4417..4793
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="POA77_004660"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1154 a         1193 c         1417 g         1177 t
ORIGIN      
        1 actaattccc gagttcattg ccgttacggc ataaatgaaa ctcataaata acaccttccc
       61 cataataata aaaatcagca taagtacccg aggtaaataa aatgattctg gacaccgttg
      121 acgaaaaaaa gaaaggcgtg catacccgct atttaatatt actgattatt tttattgtta
      181 ccgccgttaa ctacgccgat cgtgcaacgc tgtctattgc tggtaccgaa gtggcaaaag
      241 agttgcagtt aagtgcggtt tcgatgggtt acatcttttc tgcttttggc tgggcctact
      301 tgctgatgca aatccccggc ggctggctgc ttgataagtt tggctcgaaa aaagtttata
      361 cctatagcct ctttttctgg tcgctattca ctttcctgca aggctttgtt gatatgttcc
      421 cgctggcctg ggcagggatc tccatgttct ttatgcgctt tatgctcggc ttctcggaag
      481 cgccatcatt cccggcgaac gcccgtattg tcgccgcctg gttcccgacg aaagaacgtg
      541 gtactgcctc cgccatcttt aactcggcgc aatatttctc gctggcgctc ttttcgcctc
      601 tgcttggctg gctgactttc gcctggggct gggagcacgt ctttaccgtt atgggggtga
      661 ttggttttgt gctgacggcg ctgtggatca aattgattca taacccgaca gatcacccac
      721 gtatgtctgc ggaagagctg aagtttatct ctgaaaatgg cgcggtggtc gatatggacc
      781 acaaaaagcc gggcagcgcg gcagcaagcg gtcccaaact gcattacatc aagcaattgc
      841 tctctaaccg catgatgttg ggcgtatttt tcggacaata ttttatcaac accatcacct
      901 ggttcttcct cacctggttc ccgatttatc tggtgcagga aaaaggcatg tcgattctga
      961 aagtgggtct ggtcgcctcg attccggcac tgtgtggttt tgcgggcggc gtgctgggag
     1021 gtgtcttctc ggattatctg atcaaacgcg gtttatccct gaccctggca cgtaagctac
     1081 cgattgtgct gggaatgttg ctggcttcca ccatcatctt atgtaactac accaacaaca
     1141 ccacgctggt ggtcatgctg atggcgctgg ctttttttgg caaaggattt ggtgctctgg
     1201 gctggccggt gatttctgac accgcgccga aagagattgt tggcctctgt ggcggcgtct
     1261 ttaacgtctt tggcaatgtt gcctccattg tcactccact ggtgattggc tacctggtaa
     1321 gtgaactgca ctccttcaat gcagcactgg ttttcgtggg atgttcagcg ctgatggcga
     1381 tggtctgcta cctcttcgta gttggcgaca ttaaacgtat ggaattgcag aaataagcaa
     1441 aggtataagc gatgaataac gatgttttcc cgaataaatt caaagccgca ctggctgcga
     1501 aacaggtaca aattggttgc tggtcagcac tctctaaccc gattagcact gaagttcttg
     1561 gtttggctgg gtttgactgg ctggtgctgg atggcgaaca tgcgccaaac gatatctcca
     1621 cgtttattcc gcagttaatg gccctgaaag gcagcgccag cgcgccagta gtgcgagtgc
     1681 cgaccaacga gccggtaatt attaagcgtc ttctggatat cggtttctat aacttcctga
     1741 ttccttttgt agaaacaaaa gaggaagcag agcaggcggt ggcatcaacc cgttacccac
     1801 cggaaggcat tcgcggcgtc tccgtttctc accgcgccaa tatgtttggc accgtggcgg
     1861 attatttcgc tcagtcgaac aagaacatca ccattctggt ccagatagaa agtcagcagg
     1921 gcgtagataa cgtcgatgcc attgccgcta ccgaaggtgt agacggtatc ttcgtcggcc
     1981 ccagcgatct ggctgcggca ttaggccatc tcggcaatgc ctcgcacccg gatgtacaaa
     2041 aagcaattca gcacattttt aaccgtgcca gtgcgcacgg caaacccagc ggtattctcg
     2101 cgccggtcga agccgatgcg cgtcgttatc tggaatgggg cgcgacgttt gtggctgtcg
     2161 gcagcgatct tggcgtcttc cgctctgcca ctcagaaact ggctgatacc tttaaaaaat
     2221 aaccaccacc gcaacaagag agatgattga tatgactatg aaagttggtt ttattggcct
     2281 ggggattatg ggtaaaccaa tgagtaaaaa ccttctgaaa gcaggttact cgctggtggt
     2341 tgctgaccgt aacccagaag ctattgctga cgtgattgct gcaggtgcag aaacagcgtc
     2401 tacggctaaa gcgatcgctg aacagtgcga cgtcatcata accatgctgc caaactcccc
     2461 tcatgtgaaa gaggtggcgc tgggtgagaa tggcatcatt gaaggcgcga agccaggtac
     2521 ggtattgatc gatatgagtt ctatcgcacc gctggcaagc cgtgaaatca gcgaagcgct
     2581 gaaagcgaaa ggcattgata tgctggatgc tccggtgagc ggcggtgaac cgaaagccat
     2641 cgacggtacg ctgtcagtga tggtgggcgg cgacaaggct attttcgaca aatactatga
     2701 tttgatgaaa gcgatggcgg gttccgtggt gcataccggg gaaatcggtg caggtaacgt
     2761 caccaaactg gcaaatcagg tcattgtggc gctgaatatt gccgcgatgt cagaagcgct
     2821 aacgctggca accaaagcgg gcgttaaccc ggacctggtg tatcaggcaa ttcgcggtgg
     2881 actggcgggc agtaccgtgc tggatgccaa agcgccgatg gtgatggacc gcaacttcaa
     2941 gccgggcttc cgtattgatc tgcatattaa ggatctggca aatgcgctgg atacttccca
     3001 cggcgtcggc gcgcaactgc cgctcacagc tgcggttatg gagatgatgc aggcactgcg
     3061 agcagatggt ttaggaacgg cggatcatag cgccctggcg tgctactacg aaaaactggc
     3121 gaaagtcgaa gttactcgtt aatgacgtcg tgcccggatg gcgtattgca atccgggcct
     3181 ggaatccagg ccgaataaga gaaacgccct ccggcgtcat gtggtaacag gcataggtat
     3241 gaaaatcgta atcgccccag actcttataa agaaagttta tctgccagcg aggttgcgca
     3301 ggcgatagaa aaaggatttc gggaaatttt tcctgatgca cagtacgttt ctattccggt
     3361 tgccgacggt ggcgaaggaa cggtggaagc gatgattgca gccacccagg gttctgaacg
     3421 tcacgcctgg gttacagggc cgctgggcga gaaagtgaat gccagttggg ggatctccgg
     3481 cgatggcaaa accgcgttta ttgaaatggc ggcggccagt gggctggagc tggtacctgc
     3541 ggaaaaacgc gatccactcg tgaccacttc acgcggcaca ggcgagttaa tcctgcaggc
     3601 gctggagagc ggcgcgacaa acattattat cggcattggc ggtagcgcta caaatgatgg
     3661 cggcgcaggc atggtacagg cgctgggggc gaaattatgc gacgccaacg gcaatgaaat
     3721 tggttttggc ggcggtagtc ttaatactct gaatgatatt gatatttccg gcctcgatcc
     3781 gcgcttaaaa gattgcgtca ttcgcgtcgc ttgtgatgtc accaatccgc tggtgggcga
     3841 taacggcgca tcgcgcatct ttggcccaca aaagggagcc agtgaagcga tgattgttga
     3901 gctggacaat aacctctctc actatgccga tgtcattaaa aaagcgctgc atgttgatgt
     3961 gaaagatgtc cccggcgcag gagctgcggg cggtatgggt gcggcgctga tggcgtttct
     4021 tggtgcggaa ctgaaaagtg gtattgaaat cgtcactacg gcgctgaatc tggaggaaca
     4081 tattcacgat tgtacgctgg tgatcaccgg tgaagggcgt attgacagcc agagtattca
     4141 cggcaaggta ccgattggcg ttgcaaacgt ggcgaagaag taccataaac cggtgattgg
     4201 cattgcgggt agcctgaccg atgatgttgg cgttgtacat cagcatggca tcgatgcggt
     4261 cttcagcgta ttgaccagca taggtacgtt ggacgaagca ttccgcgggg cttatgacaa
     4321 tatctgccgt gcttcacgta atatcgccgc gacactggcg attggaatgc gcaacgcggg
     4381 gtgacaaggg cgcgcaaacc ctctatactg cgcgccgaag ctgaccagac agtcgccgct
     4441 tcgtcgtcgt cctcttcggg ggagacgggc ggaggggagg aaagtccggg ctccataggg
     4501 cagggtgcca ggtaacgcct gggggggaaa cccacgacca gtgcaacaga gagcaaaccg
     4561 ccgatggccc gcgcaagcgg gatcaggtaa gggtgaaagg gtgcggtaag agcgcaccgc
     4621 gcggctggta acagtccgtg gcacggtaaa ctccacccgg agcaaggcca aataggggtt
     4681 cataaggtac ggcccgtact gaacccgggt aggctgcttg agccagtgag cgattgctgg
     4741 cctagatgaa tgactgtcca cgacagaacc cggcttatcg gtcagtttca cctgatttac
     4801 gtaaaaaccc gcttcggcgg gtttttgctt ttggaggagc ggaaagatga atgactgtcc
     4861 acgacgctat acccaaaaga aagcggctta tcggtcagtt tcacctggtt tacgtaaaaa
     4921 accgcttcgg cgggtttttg c
//
LOCUS       DAMZII010000229         4874 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZII010000229 DAMZII010000000
VERSION     DAMZII010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.231"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(65..826)
                     /gene="kduD"
                     /locus_tag="POA77_004661"
     CDS             complement(65..826)
                     /gene="kduD"
                     /locus_tag="POA77_004661"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDI6367981.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(856..1692)
                     /gene="kduI"
                     /locus_tag="POA77_004662"
     CDS             complement(856..1692)
                     /gene="kduI"
                     /locus_tag="POA77_004662"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDI6367982.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1979..3160)
                     /locus_tag="POA77_004663"
     CDS             complement(1979..3160)
                     /locus_tag="POA77_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI6367983.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTSVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3415..4644
                     /locus_tag="POA77_004664"
     CDS             3415..4644
                     /locus_tag="POA77_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6367984.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1278 a         1236 c         1061 g         1299 t
ORIGIN      
        1 atgatgatct acaggtataa aaaaccctgc catgcggcag ggtcataaaa gtaagaagaa
       61 tgaattaacg cgccagccaa ccgccatcca cggcaatggt ataaccattc acataatctg
      121 aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt ccccaacgac
      181 cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt agttgttgag
      241 tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc
      301 attcgttcgc catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag
      361 gcacacggat cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat
      421 tgccttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat
      481 tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa
      541 tcaatccggc gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca
      601 gcagtccagg aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc
      661 ccagcgccgt gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt
      721 cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag
      781 tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca ataccttatt
      841 cgtttatgcc cacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
      901 ctggttttca ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
      961 aatcgaccag ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
     1021 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
     1081 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
     1141 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
     1201 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
     1261 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
     1321 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt tacccacata
     1381 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
     1441 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
     1501 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
     1561 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct cttcaaccaa
     1621 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
     1681 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
     1741 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
     1801 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
     1861 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
     1921 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
     1981 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
     2041 gtcctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
     2101 cggaagcgcc tatcgggtga ccaagtgcga tcgcaccacc attgacattg acccgacgct
     2161 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
     2221 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
     2281 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcgaatgc
     2341 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
     2401 ctgcgccatc gtttatggat gatgcattac ccgctgtcac agaaccgaga ctatcaaatg
     2461 aaggatttaa acgggctaaa ccttctgcac tggcatcagt gcgtggctgt tcatcggtat
     2521 caacaaccaa cgtctgaccg ttactttggg tcattaccgg gacgatctca tctttaaatc
     2581 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
     2641 attgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
     2701 cattgaaggc atcccataac ccatcatgca caagactgtc aaccagctgg ctattgccaa
     2761 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
     2821 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
     2881 ctttaagtcc ggaaccgcag acgtcattaa tcgtgattgc agaaacgcta ttaggcagac
     2941 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
     3001 caagaattac ttcatccacc gcatatgcag gaacgctggt acgttctatt aacgctttca
     3061 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccgcga aagcagccga
     3121 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
     3181 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
     3241 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
     3301 caaccactaa cccaccaata gaggggtaga tagggcgtta atctcccata cttaacctgg
     3361 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
     3421 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
     3481 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
     3541 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
     3601 ctctcatcga aaacatcagc aggtgaaggg ataacgggcg cagtaacaca ctactatggc
     3661 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
     3721 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
     3781 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taatcctgaa tctcattttt
     3841 ctgatgggac gccatgcaac cattcgggta atgggttttt tggtattccc attgattgcc
     3901 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
     3961 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
     4021 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
     4081 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
     4141 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
     4201 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
     4261 gcatggctgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
     4321 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
     4381 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
     4441 atcaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc
     4501 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
     4561 gcgcttaaac cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
     4621 gtatcggtaa tgttctttag ctgatatcca ttgtgaatgg ttcaaaattg ctacaatttt
     4681 gaaccattcc agttttcata atgttcattc gttattattc cgcactatca cttccctcat
     4741 catatcctag tattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
     4801 ctatttaaga tgcataactt agattcgcaa gatatcttcc ggtcattcaa aaacaatttg
     4861 cgatatagat attc
//
LOCUS       DAMZII010000230         4686 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMZII010000230 DAMZII010000000
VERSION     DAMZII010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.232"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(334..615)
                     /locus_tag="POA77_004665"
     CDS             complement(334..615)
                     /locus_tag="POA77_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367985.1"
                     /translation="MKNTAINISTKKPLQTVKDPVTRVSIHCTAKDEKGADVEVNILD
                     LEQHHTTNEIKLTFGGSPSQALTMLSRVMVVLQQQAAEELQRNSCSRLQ"
     gene            complement(681..1886)
                     /locus_tag="POA77_004666"
     CDS             complement(681..1886)
                     /locus_tag="POA77_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6367986.1"
                     /translation="MSTVENDVKTANNDISLRTYLERTGNGARKSNGLVINPAVVEEE
                     EGFNTRTAGIGESYYSLPDVVAHLNSLKESYKIDPLSVQAIVVQILNGRPVLRQGACR
                     IRAVAMANIELADEGRELITLIRCEEFRGSRASAEQFTLDGNSNLALSVVAEALSIKR
                     MVEDAEEPKTFAELAKRRGKTEQHLRQMVRVLDLPEALQAMLVKGEVSMYVALDEYLY
                     SGDEAVNNITKAIDVYGKATAKTLKFVKAGNIDAAMKPKAAIVPDANATPPETTVTEQ
                     TSGEGTNDETTPPQLVDGSAGTNPEPTPERTPAPQMKDPVKTPEPKAVSVSSVLNKKT
                     VTTIADTAIPLFSRIAEEAKQRAAKNLSDDTYTLVLTNDEMNAICEAHGDLTQYLRKH
                     AEKQNKGQA"
     gene            2370..2570
                     /locus_tag="POA77_004667"
     CDS             2370..2570
                     /locus_tag="POA77_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367987.1"
                     /translation="MIEHRTEMRQTAIKSLQEAEEALTALAMSYELQPDDKASSCHPR
                     TGTLSTASQVRKLRRVVEKQKT"
     gene            complement(2742..2900)
                     /locus_tag="POA77_004668"
     CDS             complement(2742..2900)
                     /locus_tag="POA77_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI6367988.1"
                     /translation="MKLPNQPVVLCVLIVCLTLLIFTWLTRNSLCELRLKDGTREVSA
                     VMDYESGK"
     gene            complement(3258..3863)
                     /locus_tag="POA77_004669"
     CDS             complement(3258..3863)
                     /locus_tag="POA77_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367989.1"
                     /translation="MNQTETGPSGFTETTVDEERIAIRVMIGDVVGRYRKQVPPDRVQ
                     KATFEVNGGLLMALLLALLMLVCIIQRLATGDRLMSFTGEPDVRNIQFTLMLVIEFAL
                     GMVVWFLIAMGEFSRPWLYRLTPAKPVWLFDEVEDADLVLLSGNPFIKRWLHKEILKI
                     RRLTYTRLDEELEKIAHYPDSLRHSSVRAHRLSLIAGTKTD"
     gene            complement(3940..4644)
                     /locus_tag="POA77_004670"
     CDS             complement(3940..4644)
                     /locus_tag="POA77_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDI6367990.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT         1098 a         1229 c         1154 g         1205 t
ORIGIN      
        1 ccagattggt tttcataata cctcctatgt ttttggtatt atataaaacc cgggctgctg
       61 gctaataacc tacactgaaa attttttaaa ttaaccgaca atcttaaaaa ttggacgcat
      121 gtggaattat gtaaataggt agcagatttt attaactagc ttccataggt ggtgcgtttt
      181 tagggagttt tttgccagac caacaaattc tagtagctac acattcactc gcagagatta
      241 atcgctttaa cataattagt gccagcactg agtcctttaa aattataaat ccatgttcta
      301 tccataacgt cgtgccattg ctcctttcgg tgttcactgc aaacgggagc aggaattccg
      361 ttgcagttct tcggccgcct gctgttgcag cacgaccatc actctgctga gcattgtcag
      421 cgcctgggaa ggggaaccgc caaacgtcaa tttgatttcg ttggtggtgt ggtgctgttc
      481 caggtcgaga atatttactt ccacgtctgc gcccttctca tccttagccg tacaatgaat
      541 gctgacccgg gtcaccgggt ccttaactgt ctgcaatggt ttcttcgtgc tgatgttgat
      601 tgcggtattc ttcattgccc tgtttctcct tgaccagtgg tggtaaccga ccctaccggg
      661 tggcggggtc agtcaggaaa tcatgcctgg cccttgttct gcttttcagc atgtttacgc
      721 aggtactgcg tcagatcgcc gtgagcttca caaatggcat tcatctcgtc attggtcagc
      781 accagagtgt aagtatcgtc gctgaggttc ttagcggccc gttgtttagc ttcttccgca
      841 atacgcgaaa acagcgggat tgccgtgtcc gcaatcgtcg tcacggtctt tttgttcagt
      901 acgctggaga ccgacacagc tttaggctct ggcgttttga ccgggtcttt catctgcggt
      961 gccggggtcc tttccggggt cggctccggg ttagtgcccg cactcccgtc caccagctgc
     1021 ggtggggtgg tctcatcgtt agtaccttcg ccggacgttt gttcagtcac ggtggtttcc
     1081 gggggggtcg catttgcgtc cggcacgatg gctgctttcg gcttcatcgc tgcatcaatg
     1141 ttgccagctt tgacgaattt cagggttttg gccgtcgcct taccataaac atcaatggct
     1201 ttggtgatgt tgttgaccgc ttcatcgccg ctgtaaaggt attcatccag cgcgacatac
     1261 atactcactt cacccttcac caacatggcc tggagggctt caggcagatc caggacgcga
     1321 accatctgac gcaggtgctg ttcggtttta ccgcgacgct tcgccagttc agcgaacgtt
     1381 ttgggttctt ccgcgtcttc aaccatccgt ttgatggaga gcgcctcagc tacgacagaa
     1441 agagccagat ttgagttacc gtccagcgta aactgctcgg cactggcgcg gctaccgcga
     1501 aactcctcgc atcgaattaa cgtgatcaat tcacgccctt catctgccag ctcgatgttt
     1561 gccatcgcga cggcacggat acggcatgct ccctggcgca ggaccggacg gccattcaga
     1621 atctgtacga caatagcttg tacgcttaaa ggatcgatct tgtaggactc tttcaggctg
     1681 ttcaggtggg ctaccacgtc aggtaatgag tagtaactct ccccaatgcc agcagtacgg
     1741 gtgttaaacc cctcttcctc ttcgaccact gccgggttaa tgactagacc attgcttttg
     1801 cgtgcgccgt taccggtacg ctccaggtag gtgcgcaggc tgatatcgtt gttagcggtt
     1861 tttacatcat tttcgacagt ggacatggtg ccttcctttt agttttcgtt tagggtgttt
     1921 gttcgctaca aaacaacaat accaggttcg attcatttcc taataacctg aggcgaaaaa
     1981 aaagtaactt tttttccggg ggttatagta cgcgatccgt ccgttttaaa ttctcgctaa
     2041 ggagggcaaa tttcacttta gtgtgaaaag cgggtacata aagcaggaat atgtcgaaaa
     2101 agttactttc ccggttgcct gaaaaaatgc cgttgcagca aacacaaggt gtgaacaccc
     2161 cccccgcccc ggcggctccg ggcaaaggag tatccgatga agctttcccg gcagaccaca
     2221 aatgatacgg ctgtaatcga aggcacccgc agattaccga ccatatcgcc tcccagcgag
     2281 ccaccgaagc caaaaaagac ggcatgaatt ttaatgcagg cgttgagctt gcatctaaaa
     2341 ggaactgtgc aacgaggaca aacattacca tgattgaaca ccgcacagag atgcgccaaa
     2401 cggccattaa atcgcttcag gaagccgaag aagcattgac ggcactggct atgtcctatg
     2461 agctacagcc cgatgataag gccagttcgt gtcatccacg gaccggtact ctctccacgg
     2521 ccagccaggt acggaaattg cgccgtgtcg tcgaaaagca gaaaacttga aattcgacaa
     2581 ggcggcgggt agctatttcc tgcatgtaca aactaacaag ggtttagccg acgatagaaa
     2641 ggaaaaaagg gtttccccga ccggagaaac ccttttctaa aggtgccatt agcttgctgg
     2701 cccacattct gaaatgaggg gggtcgcccc ccctctttca gctacttacc ggattcgtag
     2761 tccatcacag ccgacacttc ccgtgtaccg tcttttagcc tcagttcgca gagcgaattg
     2821 cgcgtaagcc aggtgaatat caacagcgta agacatacta tcaatacgca caaaacaacg
     2881 ggttggttcg gcagtttcat ggccttcttc tccttgcatt taagcgggta agaggctact
     2941 ctgaatgtga ctagcataca ggggcctcgg gttaatttga aaaaattact cggggctttc
     3001 tgctttttac cacagtcaaa cagtgaatct gctggctggg gcaaaagcct ccagcaccga
     3061 ggttattata ccaccccgcc ccttttaaac gaacagatcg ttctcctaaa tatttaataa
     3121 gtaagtattt caggttttgc tgcttcgcag gctgtaacag caggtgtctg acgtaataac
     3181 tgattaaaca gacggattcg aatctcaaca ctcaacgacg ccagctcgcc tgttcccccg
     3241 cttctttccg gctgtaatca gtccgttttc gtaccagcga tcagtgacaa acggtgagca
     3301 cgaacactac tgtgccggag gctgtccggg taatgggcga ttttctccag ttcctcatcg
     3361 aggcgggtat acgtcagcct cctgattttc aagatttcct tgtgaagcca gcgtttgata
     3421 aagggattgc cggaaagcag taccaggtct gcatcctcca cttcatcaaa aagccagacg
     3481 ggttttgccg gagtaaggcg gtagagccag gggcgggaaa actcccccat tgcaatcaga
     3541 aaccagacaa ccatgccaag tgcgaactcg atgaccagca tcagggtaaa ctgaatattc
     3601 cggacgtcag gctctccggt aaagctcatt aggcggtcgc cagttgccag acgctgaata
     3661 atacagacca gcatcagcag agccagtaac agcgccatga gcagcccgcc gttcacttca
     3721 aaagtagctt tctggacgcg atcaggcggc acctgcttac ggtagcggcc gaccacatca
     3781 ccaatcatta ccctgatggc tatgcgttct tcatcgaccg ttgtttcggt aaatccggac
     3841 gggcccgttt ctgtttgatt catgtaccct ctcctctctc tgaaaaaggc actgttgcaa
     3901 agttagcgat gaggcagcct tttgtcttat tcaaaggcct tacatttcaa aaactctgct
     3961 taccaggcgc atttcgccca ggggatcacc ataataaaat gctgaggcct ggcctttgcg
     4021 tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa gccgtcttca tggatttaaa
     4081 tcccagcgtg gcgccgatta tccgtttcag tttgccatga tcgcattcaa tcacgttgtt
     4141 ccggtactta atctgtcggt gttcaacgtc agacgggcac cggccttcgc gtttgagcag
     4201 agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg atgaatcgcg ggatctgcca
     4261 cttcttcacg ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg
     4321 ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc
     4381 ccagcggcca ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg
     4441 gttacgccag taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg
     4501 gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg
     4561 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     4621 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     4681 tcacca
//
LOCUS       DAMZII010000231         4612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMZII010000231 DAMZII010000000
VERSION     DAMZII010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.233"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2419
                     /locus_tag="POA77_004671"
     CDS             <1..2419
                     /locus_tag="POA77_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6367991.1"
                     /translation="SAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNALINN
                     KRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYPIEKEKQIT
                     TYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNS
                     QNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTI
                     GQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIY
                     QENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAM
                     GKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGS
                     LSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYIN
                     DTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDI
                     GPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWY
                     DSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASW
                     NGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYI
                     TTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPL
                     GISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVI
                     LPCISQN"
     gene            2435..3184
                     /locus_tag="POA77_004672"
     CDS             2435..3184
                     /locus_tag="POA77_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6367992.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3186..4250
                     /locus_tag="POA77_004673"
     CDS             3186..4250
                     /locus_tag="POA77_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6367993.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4293..4493)
                     /gene="glgS"
                     /locus_tag="POA77_004674"
     CDS             complement(4293..4493)
                     /gene="glgS"
                     /locus_tag="POA77_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDI6367994.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         1423 a          964 c          929 g         1296 t
ORIGIN      
        1 gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc gtgaaaacgt
       61 taacctttcc caatttgaaa ctgatggcca attacccgta ggtaaatact cattaaacgc
      121 tcttattaat aataaaagga cgccaatcca tctggacctg caatgggtat taattgataa
      181 ccaaactgca gtttgtctga ctccagagca attaacatta ttaggcttta ctgatgagat
      241 tattgaagtg gctcagcaaa acctgatcga tggttgttac cctatcgaaa aagaaaaaca
      301 aattacaact tatctcgata aagggaaaat gcaattatcc atatctgcac ctcaggcatg
      361 gttaaaatac aaagatgcaa actggacgcc tcctgaactt tgggatcatg gtattgctgg
      421 ggcatttctt gactacaatt tatatgcctc tcattatgca ccacatcagg gcgataattc
      481 acaaaatata agttcctatg ggcaggctgg ggttaatctt ggggcctggc gcctgcgtac
      541 tgattaccag tacgatcaat catttaacaa tggcaaaagc caggcgaaca acctggattt
      601 tccgcgtatt tatttgtttc gcccaatccc agcaattaat gcaaaactaa ctataggtca
      661 atacgatact gaatcctcta ttttcgactc tttccatttt tctggcgttt cgttgaaaag
      721 cgatgagaat atgttgccgc ctgatctacg tggttacgca ccgcaaatca cgggtgttgc
      781 acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt attatttatc aagaaaatgt
      841 tcctcctggc ccatttgcta ttactaattt attcaataca ttacaggggc aactggacgt
      901 caaggttgaa gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta atagtattcc
      961 ttatctgacg cgtaaagggc agattcgcta caccactgct atgggtaaac cgaccagcgt
     1021 tggtggtgat tccttacaac aacccttctt ctggactggt gaattctcat ggggttggct
     1081 gaacaatgta tccctgtatg gtggttcagt tttaacaaac cgtgattatc aatctctggc
     1141 tgccggcgtt ggctttaacc ttaactcatt gggttcatta tcttttgatg tcacacgatc
     1201 tgatgctcag ttgcataatc aggataaaga aacgggttat agctaccgcg ctaactattc
     1261 aaaacgtttt gaatctaccg gtagccagct cactttcgct ggttaccgtt tctctgataa
     1321 aaactttgtg acaatgaatg aatatatcaa tgacactaac cattacacga attatcagaa
     1381 tgaaaaagag agttatattg tcacgtttaa ccagtatctt gaatcattaa ggttaaatac
     1441 atacgtaagt ttggctcgta atacttactg ggacgccagc agtaatgtga attattcatt
     1501 atcacttagc cgcgattttg atatcgggcc attaaaaaac gtctccactt ctctaacatt
     1561 tagccgaata aactgggaag aagacaacca ggatcaactg tacctaaata tttcgattcc
     1621 ctggggaacc agtagaacat tgagctatgg tatgcaacga aatcaggata acaagatttc
     1681 gcatactgct tcgtggtatg actcttccga tcgaaataat tcctggagcg tttctgcttc
     1741 aggcgacaat gatgaattca aagatatgaa agcgtcacta cgcgccagtt atcagcataa
     1801 taccgagaac ggtcgtctct atctctccgg gacatcacag cgagacagtt attattctct
     1861 gaatgccagt tggaatggtt cattcacggc gactcgtcac ggagccgctt tccatgacta
     1921 tagtggtagt gctgactcgc gttttatgat cgacgcagat ggtgcagaag atattccgtt
     1981 gaacaataaa cgcgcggtaa ctaatcggta tggcatcgga gttattccat cagtcagcag
     2041 ttacataaca acatcattaa gcgttgatac ccgaaatctt ccagaaaatg tggatatcga
     2101 aaactcggtt atcaccacca ccttaaccga aggtgctatt ggctacgcca aacttgatac
     2161 ccgcaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc acccaccact
     2221 ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg ctgatggcgg
     2281 ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct ggggtgataa
     2341 atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg ttattttacc
     2401 gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca caaagaatct
     2461 cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg ccatcatccc
     2521 ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa aacttactaa
     2581 tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga aaggcaataa
     2641 atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca gtgagcaaat
     2701 tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca gagaaacgct
     2761 cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg tgatgcaagt
     2821 cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat tagaagatga
     2881 tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt cgatccgctt
     2941 taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg gaatcacaat
     3001 gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata cgcagtttaa
     3061 aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat taagttttta
     3121 tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca aacgagataa
     3181 ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt gcattacctg
     3241 tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc attggttacg
     3301 taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg caaagcagtg
     3361 agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct ggagaagaat
     3421 acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac ttttataatc
     3481 agaactccta tatttttggc gtaacataca atggagttga ttatgattcg actgccccta
     3541 atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt aataatcact
     3601 ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta acctttccag
     3661 ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat aaaaacctgg
     3721 tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca ggagctatag
     3781 aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat gtccttgact
     3841 gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt aaaataaatt
     3901 cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg acaaaagatc
     3961 ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact acggatattt
     4021 tgagtgatta tagccatctg gatatgggta atggactgct attgaagata tttcataaag
     4081 atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca tcgtctagtg
     4141 caccttcggt caccgcacca ttcagggcag aacttagtgc gaatccggca gagactgttg
     4201 ttgagggacc gtttagtaaa gacgtcatcc tcaaaatcac ctataactag catctaatac
     4261 aaacactaaa acgggccatc aggcccgtta tatcagtgct ctaactccag ctctcttgcc
     4321 tgcatcatct gttgacaata ccaggcataa cgtgcgcaaa accaggttct atgttcttca
     4381 tccatgttac cagtaacggc aagaatatcg accgggcgac cttctgcgtg cattctggca
     4441 aaactacgcg ccaggaaatc gaaattattt aaagaattaa gactatgatc cataagcaca
     4501 tcctccggtt atcgcatacc ttttaaggcc atatgctttt tctgatttca gtattcccca
     4561 atcagtgtag tgcctttaaa cataacgtgc gtaaatatat tgtccccgat ca
//
LOCUS       DAMZII010000232         4604 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMZII010000232 DAMZII010000000
VERSION     DAMZII010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.234"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..365)
                     /locus_tag="POA77_004675"
     CDS             complement(<1..365)
                     /locus_tag="POA77_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367995.1"
                     /translation="MKFILMFFILAILIWGFTGLTFKDAILFTSVGLSISVVALSALL
                     VVALTFVMAAMYAAHELGSLGKMIISQVVLVGFWNLIFSVIPDGKLISFFGVNVWQSG
                     SYLVYFMTVLVAVFAAYIAL"
     gene            complement(452..700)
                     /locus_tag="POA77_004676"
     CDS             complement(452..700)
                     /locus_tag="POA77_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367996.1"
                     /translation="MTSNRCREIKTPPEHIIADYILRFMKNNKEAKLYEAMSRLESKI
                     KLFVADGHDELHIRALLNQAAHSHTKESLKQACERICN"
     gene            complement(738..959)
                     /locus_tag="POA77_004677"
     CDS             complement(738..959)
                     /locus_tag="POA77_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367997.1"
                     /translation="MDKYLALDRALNEILTPVPTPFSTLFAGEIKAECHRIAAGESNP
                     QPSHILDRRLLALCKAGQINYTTGKGWLK"
     gene            complement(1135..1488)
                     /locus_tag="POA77_004678"
     CDS             complement(1135..1488)
                     /locus_tag="POA77_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367998.1"
                     /translation="MSLLRLTDISMDIYDMTEKEVRMDEDKVLSFINSGDGDAGDLLD
                     DAMPTAARRFYRLTQSMHTLLGEVREHFPDAMFYSANGKVSLLLGSSHNKHDQPMQEM
                     VAVSATQLHIEGGDW"
     gene            complement(1504..2112)
                     /locus_tag="POA77_004679"
     CDS             complement(1504..2112)
                     /locus_tag="POA77_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6367999.1"
                     /translation="MFLHLVPKILHPMGNLCTLDSVSVPELSLRLTGNDLVAMRPYPN
                     KQYLVGMLKGRRALNGFLVKSPRAFEEFTMVSVWNIEGFGKITHTLKTFVEDTDYDLV
                     SHDVLLAQGSYRAQASEQCRVHPVYKNIAPVHIEPKMESLLSTEPNFENDVCETHSWG
                     MLVRSRDEGFKAMTMPSARLQESVALRGDRQPQLEQAIVITG"
     gene            complement(2143..2667)
                     /locus_tag="POA77_004680"
     CDS             complement(2143..2667)
                     /locus_tag="POA77_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6368000.1"
                     /translation="MKVLNNKGAVIELPNFSELLPKVESDDGRFSKPKNKISKEQRAE
                     LRLKFGGRCAYCGCPLPEKGWHADHVEPVRRDFEMVRAPAGSRVTHRARSTGKVMHPE
                     LHAIENLFPACAPCNLFKGALSVEGMRKEISRQVERARAYSVNFRTAERFGLIEVTEK
                     PVVFWFEIHQATAQ"
     gene            complement(2698..3009)
                     /locus_tag="POA77_004681"
     CDS             complement(2698..3009)
                     /locus_tag="POA77_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368001.1"
                     /translation="MNNEMSSLGTQKAEGPFVLLTLVGEGFIFDERHEIVRINGRPKQ
                     IGVKRNYFESDLGEGKAKYWTLDINEAHVFPTLDAATEQLCKLSRPHLIKIRKLIQEN
                     K"
     gene            complement(3006..3704)
                     /locus_tag="POA77_004682"
     CDS             complement(3006..3704)
                     /locus_tag="POA77_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368002.1"
                     /translation="MKTRGMLFNDEMVRAILADQKTQTRRIIKPQYSSDEWSIRPAQT
                     PRHRGHTHDWWLPTGTQPYSALRPCPYGVVGDRITVREAFSLLGNEDACAVDWNDNIV
                     MDRTEAARIYRASCEQRSGDYGLWSIPDEADWKPRTENMKFEGAWTPSIHMPRWASRI
                     ALEITDLRVERLNTISEEDAKAEGAPTECCVLGDKHFLGFRSLWKNTYGEDSWQANPW
                     VWVVNFKRITGEVV"
     gene            complement(3845..4069)
                     /locus_tag="POA77_004683"
     CDS             complement(3845..4069)
                     /locus_tag="POA77_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368003.1"
                     /translation="MPIQEKTTVFVFNACHADKAAAASANALHSLEVEYPMTLNDLSL
                     LCESVAKALDVPGGVKYEITTEPVVDGEYD"
     gene            complement(4156..>4604)
                     /locus_tag="POA77_004684"
     CDS             complement(4156..>4604)
                     /locus_tag="POA77_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368004.1"
                     /translation="KQNHFSKLKCIAISWHFNKPTGKEHLLLFVAIVAWIFPGLVFFK
                     HSLWGGLLFFFFVAGSYAFLAFPHFTHRKKIWKERLDQSLKEYQPLDLHAWAYFKDRV
                     REEGMTYKACESWMSTEASALYKSPKQEWSFLDSTSVGENDKSQPD"
BASE COUNT         1180 a         1190 c          994 g         1240 t
ORIGIN      
        1 agcgcgatat acgctgcgaa aacagctacc aacaccgtca tgaagtaaac tagatatgaa
       61 ccagattgcc agacattaac gccaaaaaac gaaattaatt ttccgtccgg gataacagaa
      121 aaaatcaggt tccagaatcc aaccagaacg acctgagaga taatcatttt accaagcgat
      181 cccagttcat gagccgcata cattgcagcc atgacaaatg tcagagctac cacaagcagc
      241 gcagacaaag ccacaaccga aatgctcagg ccgacggatg taaacagaat ggcatcttta
      301 aaagtcagac ctgtaaaacc ccagatcaga atggctaaaa tgaaaaacat aaggataaat
      361 ttcactagaa ttgcgtccct ctttcggtgg cagagtgtcg gtcaggatgc ctgtggcgag
      421 gcaactacag acagaattta acacaaaaaa tttaattaca aatccgttca caggcctgtt
      481 tcaatgattc tttggtatgg ctatgtgcgg cctgatttaa caatgcccgg atgtgtagtt
      541 catcatgacc gtcagctaca aagagtttga ttttactttc aaggcggctc atggcttcgt
      601 agagttttgc ctctttattg ttcttcataa agcgaagaat gtaatccgca ataatatgct
      661 ctggtggcgt tttaatttca cggcagcgat tgcttgtcat gagtttttcc tcaagtttgt
      721 ggcaaaatgg caccatctca tttaagccac ccttttccgg ttgtataatt aatttgacct
      781 gctttacaca gcgccagcaa acggcgatca agaatatgcg acggttgcgg attgctttcc
      841 ccggctgcaa tccggtgaca ctctgctttg atctctccgg caaataatgt cgagaacggc
      901 gtaggaactg gggtaagaat ttcgttgagt gccctgtcca gcgccagata cttatccata
      961 ttcatgtcct tgcaggtaat tgaatggatg gggtttaaat ttattatact gaatcgagtt
     1021 actaaactaa tatccttcag gatgaagaaa ggaatcatcc accttcagta tgtattcgac
     1081 atataggaca cgtctctaaa atttttcgcg ttttcgcgat ttacaccgac aggcctacca
     1141 gtcaccacct tcgatatgaa gttgcgtcgc agaaacagcc accatttcct gcattggctg
     1201 gtcatgcttg ttatgactac tcccaagcag taggctcacc ttgccgttag cggaataaaa
     1261 catagcatca ggaaaatgct ctctgacttc ccccagcaat gtgtgcatcg actgagtcag
     1321 tcggtagaat cgacgtgcgg cagtcggcat tgcatcatca aggagatctc cggcgtcgcc
     1381 gtccccgctg tttatgaaac tcagtacctt atcttcgtcc attcgaacct ctttttcagt
     1441 catgtcatat atatccattg agatatctgt cagcctgagc aacgacatca ggctgacctt
     1501 cgctcacccg gtaataacga tggcctgttc caattgtggc tggcgatccc cccgcagcgc
     1561 taccgactcc tggaggcgtg cggaaggcat cgtcattgcc ttaaacccct catcccgcga
     1621 acgcaccagc atcccccacg aatgggtttc acagacatcg ttctcgaaat tgggttcggt
     1681 cgacagcagt gattccatct tcggttcgat gtgtaccggc gcaatgtttt tataaacagg
     1741 gtgaacacga cactgttcag acgcttgtgc tcggtagctc ccttgcgcca gcagcacgtc
     1801 atgggaaacc aggtcataat ctgtatcttc gacaaaagtc ttcagggtgt gcgttatttt
     1861 gccgaatcct tcgatattcc agacggacac catcgtaaac tcttcaaacg cacggggact
     1921 tttgaccaaa aatccattca gcgccctacg ccccttcagc atccccacca ggtattgctt
     1981 gttgggataa gggcgcatcg caacaaggtc attaccagtc aggcgtagcg acagttccgg
     2041 gacgcttacg gaatccagcg tgcaaagatt tcccatcgga tgcaggattt ttggtacaag
     2101 gtgcaaaaac atcggtattc tcctcgttta ttaagccttc aattattgtg ccgtcgcctg
     2161 atgtatttcg aaccagaaga caaccggctt ttccgtgact tcaattaacc caaatcgctc
     2221 tgcggttctg aagttgacgc tgtatgccct ggcgcgttct acctggcggc ttatttcttt
     2281 cctcatgcct tcaacactta atgcaccttt aaagagattg cagggggcgc aagccggaaa
     2341 aagattttcg attgcatgca gttcagggtg catgacctta ccggtacttc gagcccggtg
     2401 tgtcactcga cttccagcgg gggctctgac catttcaaaa tcccgcctca ccggttcaac
     2461 atgatcggca tgccagcctt tttccggcaa aggacagccg cagtaggcac accgaccgcc
     2521 aaatttcaaa cgcagttcgg ctcgttgctc tttggatatt ttattttttg gttttgaaaa
     2581 tcgcccatca tcagattcaa ccttcgggag gagttctgaa aaattgggta attcgattac
     2641 tgcgcctttg ttatttagaa ctttcaccgg acctcccaac tatattaaga cgatttttta
     2701 tttattttcc tgaattagtt ttcgaatttt aatcaggtgt ggtcggctta atttgcatag
     2761 ctgttcggtc gcggcatcca gagtgggaaa tacatgggcc tcattaatat ccagtgtcca
     2821 gtatttcgct ttaccttctc ccagatcaga ttcaaagtaa ttccgcttta ccccaatctg
     2881 ctttggtctt ccattaattc tgacgatttc atgccgctcg tcgaagataa aaccctctcc
     2941 gaccaacgtc aggaggacaa aagggccctc ggccttttgc gtacccagcg aactcatctc
     3001 attattcata cgacctcccc ggtaatacgc ttaaagttga cgacccagac ccacgggttg
     3061 gcctgccaac tgtcctcgcc gtacgtgttt ttccagaggc tgcggaagcc aagaaagtgc
     3121 ttatcgccaa gaacacaaca ttctgttggt gcgccttcag ctttggcatc ttcctcgctg
     3181 atggtattta accgctctac ccgcaaatcg gtaatttcca gtgcaatacg gctggcccag
     3241 cgaggcatat ggattgaagg tgtccacgca ccttcgaact tcatattttc ggtacgaggc
     3301 ttccagtcgg cctcatcagg gatcgaccac agcccataat cccccgaccg ctgttcgcag
     3361 ctcgcccggt aaatgcgggc tgcttcagtc ctgtccatca cgatgttgtc attccagtcc
     3421 accgcgcacg catcttcatt cccgagcaat gaaaacgcct cacggaccgt aatgcggtcg
     3481 ccaactacgc cgtacggaca aggccgaagc gccgagtatg gctgggtccc tgttggaagc
     3541 caccagtcat gcgtgtgccc gcgatgtcgc ggtgtttgcg caggccttat agaccattca
     3601 tccgaactat attgaggctt tataatccgc cgcgtctgtg ttttctgatc tgccagaatt
     3661 gcccggacca tttcatcatt aaaaagcatt ccacgtgtct tcactgtgct gactcctttc
     3721 acacatggtt cacacattga gaggcctatc cctctttatc agccaggcat accgaaactg
     3781 tcggtagttt tacgggaacg ggcaactgcc cccctacctg ataaagtggt gttctccccc
     3841 aaaattaatc gtattcccca tccactaccg gctcagtcgt aatttcatat ttcactccgc
     3901 cgggtacatc caacgcttta gcgacggact cacacagcaa tgacaaatca ttcagggtca
     3961 ttgggtattc aacttccagg ctgtgcagtg cattggcgct ggcagcagca gccttgtcag
     4021 catgacaggc attaaaaacg aaaacggtgg ttttttcttg aataggcata gtgcaatcct
     4081 ctgaataatt agccctgtga tcagggcggt ttttgattaa atttaaatgt tcgttttcaa
     4141 taattagtga atctgttaat cgggttgtga cttatcattt tctccaaccg aagtgctgtc
     4201 gagaaatgac cattcttgtt tggggctttt atataatgca ctagcctcag tactcatcca
     4261 cgactcacag gctttataag tcatgccttc ctctctaacg cggtccttaa aatacgccca
     4321 ggcgtgcaga tcaagcggct gatactcttt cagcgactgg tcaagccttt ccttccagat
     4381 tttcttccga tgagtaaaat gaggaaaagc caggaatgca taactacctg caacaaaaaa
     4441 aaagaacagg agtccgcccc atagagaatg tttgaaaaac accagtcctg ggaaaatcca
     4501 tgccacaatg gcaacaaata aaagaaggtg ctccttccca gtaggtttat taaaatgcca
     4561 ggaaattgca atacacttta atttactaaa gtgattttgt ttct
//
LOCUS       DAMZII010000233         4577 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZII010000233 DAMZII010000000
VERSION     DAMZII010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.235"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(108..1280)
                     /locus_tag="POA77_004685"
     CDS             complement(108..1280)
                     /locus_tag="POA77_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004702856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6368005.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTTGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARAKRDEARSMLAKGINPSEARKANKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPDFVNA
                     LENYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAISILKKLQEITGNYSLVFPGRNDVRKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYLKQRESMMQAYSDILIARY"
     gene            complement(1441..1515)
                     /locus_tag="POA77_004686"
     tRNA            complement(1441..1515)
                     /locus_tag="POA77_004686"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1480..1482),aa:Arg,seq:cct)
     gene            complement(1591..2523)
                     /locus_tag="POA77_004687"
     CDS             complement(1591..2523)
                     /locus_tag="POA77_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6368006.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2817..3572
                     /gene="mlaA"
                     /locus_tag="POA77_004688"
     CDS             2817..3572
                     /gene="mlaA"
                     /locus_tag="POA77_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDI6368007.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3754..4428)
                     /locus_tag="POA77_004689"
     CDS             complement(3754..4428)
                     /locus_tag="POA77_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368008.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAWFESSKAIMAELFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAKYQNEINQLSAPG
                     VNFDDI"
BASE COUNT         1165 a         1020 c         1025 g         1367 t
ORIGIN      
        1 aatagtatta gctgcaatga caatagatct cgacaaaaaa ggtcagtggt atagcccatt
       61 agtagagtat ataaaaccta taactagcgt atcattctaa ggcggtgtta gtacctagcg
      121 attaaaatat ctgagtatgc ttgcatcata gattctcgtt gttttaaata ttgcgcatgg
      181 ttataagtcc ctctaatgga gtttttatca acatgcgcaa gttgcatttc aatccaggcg
      241 ctttcaaagc catgttcatg caaaatagta ctcatggtat gcctgaagcc gtgacccgtt
      301 aaccgaccat gatagccaag cagctttatc actttgttga tactggcttc gctcattggt
      361 ttcctgacat cgtttcgccc agggaaaacg aggctgtaat tcccagttat ttcttgcagc
      421 tttttcagga tgctgatcgc ttgagtagat aacggaacca gatgagggcg gcgtttcttc
      481 attcgttctt tggggatctc ccataaggca ttatctaaat caaactcagc ccattccgct
      541 gcacgtagtt caattgtgcg cactccggtt agcatcaaca gctgcgtggc atatttggtg
      601 actaaactgc cttgatagtt ttccagagca ttgacaaaat caggcaattc actttcagta
      661 aggaacggga agtgttgagt tttaggcttg ttgagtgcaa tagccaagtc aggagcaaag
      721 ttatatttag ctcgtccggt ggcgacggca taacgtaaaa cctcaccgca gcggcggcgg
      781 atcttactgg tttgttccag tgcacctctt ttctctattt tttgcaggac ggttagcagc
      841 tctagtggtt caatctgctc aattgggcgc tgaccgataa aaggaaaaat atccttttcc
      901 atacaattga gaacctcttt tgcatatccc tctgaccagg tagctttttt cgatgagtgc
      961 cattctctgg ccacagactc aaagctgttc tcatgcgcga actgcaaagc aatcttattt
     1021 gcttttctgg cttcacttgg gttgatccct ttggctaaca tcgaacgcgc ttcatcgcgt
     1081 ttagcacgtg cttgtgccaa tgacacatcg ccatagacgc cgaaggaaat catctttggc
     1141 ttacctgcaa agcgatagcg gaaacgccag cctttgctgc ccgttgtgtc gatgaggagt
     1201 gatagtccca taccatcgtt aagcgtatag ggtttgtcct tcggttttgc tctttttatt
     1261 tggatgtcag atagcagcat gtgtatagaa aaaagatcga actcagttat acacaatgtt
     1321 atacgcaaag atgtatagat tccattagaa ataatggcac tttgctggac aagtaaaagg
     1381 agatgagaac agtagaaaca gtgagttatg gaattaattg gacttagtga gaagtgttca
     1441 tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta ggaggggctc gttatatcca
     1501 tttaactaag aggacaatgc ggcatgagta tacccgctaa tggactgcgg ggtaagtacg
     1561 ctgccgctcg attgcttaaa ccctcgccat ttatgctggg tttttatcat ttttcttaat
     1621 gttttccgca cgttctgctt tttggcgtgc ttctgcttta cgcttgttgc tcatgtcgtt
     1681 acgaatctgt gcatgactca ttaacgcgaa gataaaggtg ccgccgcaga tgttccccgc
     1741 taaagtaggt agtgcgaagg gccagatgaa atcgctccag tgcagcgtgc cgttaaacac
     1801 cagatagagg atttcaacag aaccgacaac gatgtgggtg gtgtcaccca gtgcaataag
     1861 ccaggtcatc aatataatca ccacaatctt tgccgcaccc gcagcaggaa acatccaaac
     1921 catagtggcg atcagccagc cggaaatgat cgcgttggca aacatctcgc tgggggtgtt
     1981 cttcatcaca tccatgccga ttttgacaaa tgcatcgcga gtttcttcat tgaagatagg
     2041 catatattca aatgcccatg cagcaatacc tgtcccgaga atattaccca gcagcacgac
     2101 gccccataat cgcataagta agccgacgtt gctcattgtc ggtttttgca tgacgggtag
     2161 taccgcagtc acggtgtttt cggtaaataa ttgctggcgg gccataatga cgataataaa
     2221 accaaaggta taaccgagat tctccagtaa gaagctgcct ggcactcctt ccagttcgac
     2281 atgaaatatc ccttttgcca gtagcgaagc gcccatcgac agacccgccg caatggctga
     2341 ccacagtagc gccattgcgt cgcgttccag ctctttttca ccatcctggc ggatatgctc
     2401 atgaattgcc atcgcccggg aggggagtcg gtcttcatct atttctattt ttttgccgcg
     2461 ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt tgtcgttgtc
     2521 catggtctct tctcttgaat tagcacgtat agctaaagcg tagcggcttt tttgctcgca
     2581 actggcggga gttactctga aaatgtagaa aaggctgcgt ttgccttttt ctgtttctat
     2641 agaatcaagt agcctacagg gcggcgatta ccaggctatg atcaaatcag caaatcaggg
     2701 cgtctggaca tcagttgacg tgctgttaca atcgcccaca cctaaacagg cggatacggt
     2761 atcgttccgt catggatggc aaactgcata agccataaaa aaacagggag acatttatga
     2821 agcttcgcct gtcggcgctt gctctgggaa ctacgcttct ggtggggtgt gcgagttccg
     2881 gtacagatca gcaagggcgt tctgacccgt tagaagggtt caaccgcacc atgtacaact
     2941 tcaacttcaa cgtattagac ccgtatattg ttcgaccggt cgctgtcgcc tggcgtgatt
     3001 atgttccgca accggcgcgt aatggtttga gcaactttac tggcaacctt gaagaacctg
     3061 cggtgatggt taactacttc ttgcagggcg acccttatca ggggatggtc cactttaccc
     3121 gctttttcct gaacaccatt ttggggatgg gcggctttat tgatgttgca gggatggcga
     3181 acccgaaact gcaacggact gaacctcacc gcttcggtag tacgcttggt cattatggcg
     3241 tgggttatgg gccttacgtt cagttaccgt tctacggtag cttcacgctg cgtgatgacg
     3301 gtggtgatat ggcggatggt ctttacccgg ttctttcctg gctgacctgg ccgatgtctg
     3361 tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca gctgctggat tccgatggtc
     3421 tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga agcgtacttc cagcgtcatg
     3481 atttcatcgc taatggcggc gaactcaaac cgcaggaaaa tccgaacgca caagcgattc
     3541 aggatgattt aaaagatatt gattctgaat aagaaacaaa taaaaaaggt gagtcgcaaa
     3601 actcaccttt tttgttgtta tccctcaatt accacaagta aaaggagata aaccgacaat
     3661 gacccggacg taatgatgta aaaatactga tgttcgtcac tgactatttc cgatattcag
     3721 gcgttgaaaa atcaaaaaac tggaaaaata attttaaata tcatcaaaat taacacctgg
     3781 cgcagatagt tgattaattt cattttgata tttcgcctgg cgattttgaa gtcttttttc
     3841 aacgttatct tcgagatcct taaagtcttt gtgtttattt tcaggatggt caacgtcaac
     3901 tttgttagcg aataaattag aaattatttc ttttacattg tcgcgtctaa attttgcatc
     3961 gtccacttga aaattatatc ttattgctcg acctaaacgt ttaaaaactt cgttaggttc
     4021 tctcagtctt tccccgttga gctcgtttac aacctgatga tcggaacctg ttgcaaataa
     4081 ttctgccatg atagctttac tgctttcaaa ccacgctgaa gcattcctgg ctttttcact
     4141 acaatattgg gtgagactat caccattaat acgaattcct ctttgaatgt ggtagtcgtc
     4201 ctgatcgtag taaccgagag ataagtaatc atttccgttt ttatttttaa ttacaatagc
     4261 gagagagtcg ccttttttat ctttaccgta ccaggtaaat aagtccgttg gttcgctatt
     4321 cccgctctca tcaaattccg ttaccgtatg tctgagtttg tgtgatatct tatcgaagat
     4381 aaaaccagga tctccatcat atatttcttt taatgcgctg aagatcattt ccttgttatt
     4441 gatatcatga gtcttctcaa ctatgttgtc attaatgata gtacggacga gttctttgtc
     4501 tttatagttc gcaggtaatt ctattggttc ttggttgtcg ataccaatag taatatggtc
     4561 tgtatcattg gagccaa
//
LOCUS       DAMZII010000234         4541 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZII010000234 DAMZII010000000
VERSION     DAMZII010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.236"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..184)
                     /locus_tag="POA77_004690"
     CDS             complement(<1..184)
                     /locus_tag="POA77_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368009.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPR"
     gene            488..1420
                     /gene="rfaD"
                     /locus_tag="POA77_004691"
     CDS             488..1420
                     /gene="rfaD"
                     /locus_tag="POA77_004691"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDI6368010.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1430..2476
                     /gene="rfaF"
                     /locus_tag="POA77_004692"
     CDS             1430..2476
                     /gene="rfaF"
                     /locus_tag="POA77_004692"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDI6368011.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2480..3472
                     /gene="rfaC"
                     /locus_tag="POA77_004693"
     CDS             2480..3472
                     /gene="rfaC"
                     /locus_tag="POA77_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDI6368012.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3469..>4541
                     /gene="rfaL"
                     /locus_tag="POA77_004694"
     CDS             3469..>4541
                     /gene="rfaL"
                     /locus_tag="POA77_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDI6368013.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYR"
BASE COUNT         1228 a         1021 c         1087 g         1205 t
ORIGIN      
        1 cccttggttt caaatcggga gaagtgaaaa taatcatctc attttcaagt gcggccagac
       61 gctcaaagta actaaaataa acatcaactg aacgagctag tttttcacgg aaccctttat
      121 tagcagtcca atcccctcta ccaatgtcaa aatatgcagt gataattgtg gtggatgatt
      181 tcattactaa taagaccatt aatataaaaa aagaattata tcataaatat taacagaatg
      241 tatactgtat attatccatt caaatgcttc cgttatttat catacatcgg aatattgata
      301 ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa
      361 ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg
      421 tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt
      481 tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaagc
      541 cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa
      601 gtttgtgaac ctggtggatc tgaatatcgc agactatatg gataaggaag acttcctgat
      661 ccagattatg gctggcgaag agttcggcga tgtcgaagcg attttccacg aaggcgcgtg
      721 ctcttccacc accgagtggg acggcaagta tatgatggat aacaactatc aatactccaa
      781 ggagctgctg cactactgcc tggagcgtga aatcccgttc ctgtacgctt cttccgcagc
      841 cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa
      901 cgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgccaaatcc tgccggaagc
      961 gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa
     1021 aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc
     1081 gaagctgttc gaaggtagcg agaactttaa acgcgacttc gtctatgttg gcgatgtggc
     1141 agatgtaaac ctgtggttcc tggaaaatgg cgtttccggc atcttcaacc tcggcaccgg
     1201 tcgtgcggaa tccttccagg ctgtagctga tgctacgctg gcttatcaca agaaaggcca
     1261 gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc
     1321 agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg
     1381 tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact
     1441 ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct
     1501 ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt
     1561 attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct
     1621 ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc
     1681 ctacgtctta cctaactcct tcaaatccgc attagtgcct ttcttcgcgg gtattcctca
     1741 tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga
     1801 taaagaagcc tggccgctaa tggtggaacg ctatatagcg ctggcctatg acaaaggcat
     1861 tatgcgcaca gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga
     1921 aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg
     1981 tttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga
     2041 gctggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct cggcgaaaga
     2101 tcatgaagca ggcaatgaga ttcttgccgc tttaaatacc gagcagcagg catggtgtcg
     2161 gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag cctgtaaagc
     2221 cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt
     2281 tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt
     2341 gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca
     2401 ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt
     2461 acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt
     2521 tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga
     2581 ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt
     2641 tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagcgga
     2701 acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca
     2761 ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt
     2821 ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat
     2881 tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttgggcta
     2941 tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc
     3001 tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg
     3061 gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa
     3121 acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc
     3181 ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc
     3241 taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc
     3301 caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca
     3361 agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca
     3421 aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca
     3481 ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc
     3541 ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt
     3601 cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta
     3661 tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt
     3721 cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat tattgagaat
     3781 actttactat gcacaatagc aataccgatc atattgagag acgagaaaag agaagatgtc
     3841 gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg
     3901 attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt
     3961 agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa
     4021 tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta
     4081 ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg
     4141 atgtttaaac aatggaagtt actattagta ggagtaatgg ttgccatcat tgcattgtcg
     4201 gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat
     4261 agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat
     4321 cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc
     4381 attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc
     4441 atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata
     4501 ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc g
//
LOCUS       DAMZII010000235         4462 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMZII010000235 DAMZII010000000
VERSION     DAMZII010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.237"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            346..609
                     /locus_tag="POA77_004695"
     CDS             346..609
                     /locus_tag="POA77_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDI6368014.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            527..814
                     /locus_tag="POA77_004696"
     CDS             527..814
                     /locus_tag="POA77_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDI6368015.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1256..1789
                     /locus_tag="POA77_004697"
     CDS             1256..1789
                     /locus_tag="POA77_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDI6368016.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2043..2726
                     /locus_tag="POA77_004698"
     CDS             2043..2726
                     /locus_tag="POA77_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HDI6368017.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2740..3306
                     /locus_tag="POA77_004699"
     CDS             2740..3306
                     /locus_tag="POA77_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDI6368018.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            3574..3828
                     /locus_tag="POA77_004700"
     CDS             3574..3828
                     /locus_tag="POA77_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDI6368019.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            3871..4323
                     /locus_tag="POA77_004701"
     CDS             3871..4323
                     /locus_tag="POA77_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDI6368020.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIKNSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
BASE COUNT         1235 a         1071 c          971 g         1185 t
ORIGIN      
        1 accatttgtt acacaacccg ccaattgtgt aacaacagcc tctacaagag catatttgtt
       61 catatgttcg tcagaactgc ggattgtctg gacgagggtc agacaccgtt tcgtgcatat
      121 ggaccagcat gttcgaacgc cacaaagcag aagcatcatg cctggatcgc ttcggcgcgg
      181 agcgttaagt tttgacgctt ttttgtatgt tttttaagct gttagttgac tcggtgcgtt
      241 atactaagta ccctttactg gtcacaggca aaactgtgat cgggcgtagg aacccgcgta
      301 gtcatcgtcg gcgcagaagt taatttcttc atttggcgtc gttttttgcc ataccataaa
      361 gacaggaact tgtatttctt gtcgtcatct atggtagctc aggcggggca ggcattagcc
      421 tggccggttt ccgatgacgc cggtattcct acccccgtct gggctatcac caatgagcgt
      481 aggaactcgg tgatagcgtt tttagttgtc atcggagacc tttgctatgt cacctcaact
      541 gatacagaaa ctcccggcca tcactctgtt ggaaggcatg tttcccgagc tatcaacaaa
      601 ccagcttaaa gtctgcgttt tttacgcaat gggtgttcct tacgacgcca ttgctcagaa
      661 ctgtcggctt tctcccgaaa cggtacgaac ctacctgaag cgaagcctga agaatttaaa
      721 tctggaagga tatgatgcac ttcgctctgc tgtgctgatg cgaactttcg ttttcatgat
      781 tagcaataca gctaaagaaa acgaaaaaat gtgaacaggt gtccccatta aaggggacac
      841 tttcatggaa attgcatttt gcactttaca ggcgatccca aattgataaa cataaacacc
      901 aagcacaagt tactttaata aagcaaatca cttcagacgg tgtcccagtt atttgtatct
      961 ggctcatgcc cgatatatgg cgaccttact ctgaactgac gtgctgatgt tttatttcgc
     1021 gtatcacaat actgtgccac tgtattgctg atattagtgc ttagccgttc tttatctgtg
     1081 agttcgccaa atcaccgata cagaacgtac tagagtctgc tggttatatg cctgtcagaa
     1141 ctgcagttta tccagatatg ggctggacac tgcttcagac atatgattta gcatattcgc
     1201 ctgacatcgc aaaatcgtcc atcatggatc ggttttgcgc ggagcgttga acttcgtgaa
     1261 tatagaacac ctgaataacc ggaactggta tcttgcgcaa tataatacag ccggcaaaaa
     1321 cagagaatct ttattctcat ggctgaatga gcaaaacgtc gtaccatgga ctccattaat
     1381 aacaagaaag ataagacggg cagactccag gtgctgttac cgtgaaagaa tattcgctat
     1441 ttttccaggg tactttttta ttctggctaa ttttgacatt cacccagtat cagcattacg
     1501 tcgacatagc gccttcattg atttcgtgaa gtttggcggt gaaataaaac ccgtcaataa
     1561 agatattgtt gatggattaa tgaagattta cccggaccct gtgctaaatc cgggagccag
     1621 ggaagagctg aatgcggctt ccagcatatg gctgacaaaa gcacaatatc agtatctcct
     1681 cagaatggaa aatactctcc agcctgagtc gaggatatcc ctgctcctgg aactggtatc
     1741 aaatgccgaa catcacggct ttatggagcg ccttgtaaac atcccatagc cagtatttcc
     1801 cggccttcgg gccgggcttt tttcaattca agagaatatt ccggagtgaa gcctgcacga
     1861 agccatcccc accatggact ccccctgtct tttgatggaa ccgtgtattt cttaatggcg
     1921 aaagaaataa aaggcttcac cccagagtat tgattcgttt tatcaaaggc cggcccgtgc
     1981 catcacgtgt ggcggagaga acggataccg gtcttctttt catctcacag taggacattc
     2041 gcatgaccac gacagaacat cccgtgcgcc agttgctctg gtgtgcactg aatgccctga
     2101 aaacggcaca ggaaaaccac aatataacca gtgaaaccgg attacggcat tacctgcttg
     2161 aatggctttc tggtgccagc agacatcctg agttccgtag tctccctggc gaaatcatgg
     2221 cgttaaaagc attgaccgaa aaagatcgca atattcccat catcggaacg ctgaatacgc
     2281 tctttctcag ctctgccact gtcggggaat gtccgttgtt tcgttttcgg gctgcactgg
     2341 gtaaattaca gaaagcaggc tggcgcacat atgtctgccc atggcctgag agggtgttca
     2401 atgaatccat tgaacgggcc tgttccggga aacgtcatct gctacaacta agtcgcaccg
     2461 aagagtgttt tctaccgaca ggcgatatga gggcaccagt tacgttgcag ctgatcactc
     2521 cactacaccg caaaactatg cagcttctgg aagcggcaga gttaatattc tccgacgaag
     2581 gatttcaggt ggtgagagga tacgaacatc aattcgggat cgaacgcaga gaaaccctct
     2641 ttcacacctt atttatcggg ctgcccagcc tgccagaaga tgtctggggt gcaacacagg
     2701 agcagacgac tatcacatta cactgaggaa gagcaataca tgaaactcca atatcgtatt
     2761 ccattagcta tatatagcgt ctggtgcatt atagcgattt tgctctgcaa ggatggcttg
     2821 taccagatga acatatggac gctagtagag atattgggga tgatagctct cccgctgtta
     2881 gtccgtccgt ttttcattct gcttagaata atatttagga aaaatagttt taaaaccaaa
     2941 gacatgtctg acaatcacac agtatgtgtt ttcctgagcg cactcgcaga tacttcctca
     3001 aagaaggcga taaaaaaaca cttcaagcaa ttactcgaaa agctgccccc cttattacgc
     3061 cagaagaaac gtatctacat gaaatcccat cttctgactg aagccaggac acaaaagctg
     3121 atttgctctc tgcgccgaaa agggctggat gtaacagcgg aacgaaggga atccgccatg
     3181 aatagcgtca tgttccgcat cctcatcgtc agcagaatct tatctcagtg gaaagtgcct
     3241 cacataaatc cccgctgtgg tattgtcatt ctgaccctga aaaacgattc tggaaacagg
     3301 gaataacagt tataagagca ccacgatttc cgacaggaaa acatacatga aaatcaacgt
     3361 agatgaccct ggcaaaaaga gcttcgctct cattacgcct cacgtgcgtt aattcccgac
     3421 acaccccgtg acccgtcttc gcagcctgta ccaccctttc tgccgttccc gggattcttt
     3481 ctgtcactac tcgcttgtgc cgttttttcg caaccggtac gtgcagttac gacggtacag
     3541 ctgcttcttt tcccgttccg aaaggagtct ttgatgccac agcaacatcc cggacgacta
     3601 caggtactgg tcgtcgacac gcactgcaaa cgcaagctgt tttcaacgaa aacacagaca
     3661 gatcccgatg agcttgcccg acgcttctgc acgccggata actgccttgt ggtggtacta
     3721 tgcaacaacc gatttctgtt ccgcctggaa cgtgcacccg gtagccactg ccgctggcgc
     3781 aagggtagcc gctcccgcca tcagcacctg caggactggc tgtcgtaaaa acaacaaaaa
     3841 caaaagacct cgtcaaaaca ggaggggacc atgtcgtttt tatttaaatt aaccatcgcg
     3901 attttgattg tctttgcaat cctttttccg attttaattt catcagataa caaactgtcc
     3961 gcactttttt ggaagatacc aaaagatttc tgggttttcc tgattatcat gtacggggca
     4021 atcatcacgg tggtgttcat agaaagcagt gaacaggcac gcatccgccg ccaacaatat
     4081 ttgcaacagt acaaaatgcc aaatgagata catgaaaatg tggtgataaa aaacagtatt
     4141 atttcaagag aaacaacaga tgacgaatct gacattaaca ccctgtacct tgattcattt
     4201 aaaagtaaaa acaactgcaa atcttttgaa accaattcaa atttactgga taaaaatggt
     4261 aagcctgtgg aaataatggt gtgtgatgtt caggaactgc attttttcgt tccaaagaat
     4321 taaacagtta atccccctta ctctgttatc ttagtcgccg gcatcaacta acatgaaaaa
     4381 taataacgta ccattatatc tatggttact ttggtgtacg ttttgcctgt acatcactac
     4441 cccgtggcaa tacttccgtt at
//
LOCUS       DAMZII010000236         4367 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZII010000236 DAMZII010000000
VERSION     DAMZII010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.239"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..702)
                     /locus_tag="POA77_004702"
     CDS             complement(<1..702)
                     /locus_tag="POA77_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDI6368021.1"
                     /translation="MTLITQENEHDRLATRLSIILSRLFQGEKLHIGTLAEEFGVTTR
                     TLRRDFNVRLTYLDIEQSNGVYQLASHYFRRRTERDMKILARLLHLDRLLPAMDAKLL
                     SLMLDGERPSPYRIMLPEPRQKPTLFGDFSRLTLAILNQTQVRIRTDENVSHTVHPYR
                     LLCSHAEWFLAGCTSSGVFVISLSRIRLVEVLPDTTFEIDNTLISLIEQSDFMEALPH
                     MNIIHQIMHYGSKQTN"
     gene            complement(805..1554)
                     /locus_tag="POA77_004703"
     CDS             complement(805..1554)
                     /locus_tag="POA77_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3944 domain-containing protein"
                     /protein_id="HDI6368022.1"
                     /translation="MATYRHDPDLEFLRHCHRDDLDLLVSVLICDPKDGLARITETLT
                     YDPQYKKHKPNHPRYWDAIAAEVQTFGANSFATLLRWGKGVLYREILTDACDKMKVNY
                     NSKSSVETIEMNLLMKILEKSLEKMTPDQLKTVAEELQTGYSNPTPELLTMAIQAGIK
                     TSGFAAYQMALVVTNGVAKALLGRGLTVAGNAMLTRTMGAFAGPVGWVLSSLWLLIDL
                     AGPAYRVTLPSCVFIAYMRQKHLYSPENTDV"
     gene            complement(1698..2081)
                     /locus_tag="POA77_004704"
     CDS             complement(1698..2081)
                     /locus_tag="POA77_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368023.1"
                     /translation="MKIFKLLTATILLSAFSHSAFADEQADAQMIANSTFCAMYSTRL
                     TQTSDSGLQLKGVNLNARINGPVFNRVLQVMNQTYGRTWLESNARNGSMTAMQLSQSE
                     LLYNPEYARQCDAFADKVEKEWRGK"
     gene            complement(2159..2398)
                     /locus_tag="POA77_004705"
     CDS             complement(2159..2398)
                     /locus_tag="POA77_004705"
                     /inference="COORDINATES: protein motif:HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDI6368024.1"
                     /translation="MARMATMKASRCRQRVTGQGERLIRLTMALFYGQTISVRYIIRQ
                     FDVSPRTARRDLTHLAFVLESAGPHRWRLAPSLKP"
     gene            complement(2377..2841)
                     /locus_tag="POA77_004706"
     CDS             complement(2377..2841)
                     /locus_tag="POA77_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HDI6368025.1"
                     /translation="MYRLLLLNFVDSFIDNCILSCVDEPAYGGIVQCELLFGLAAHSG
                     IYVGNGDIVHLDGDGTVIKSKAKGFLDRLDGWNSAISVYTDCMDLTPQGCARAAERAK
                     AAVGQRYPYSLLDFNCHRFTARCVTGDSCPDIWLARHLDSFQKGDRWRVWQR"
     gene            complement(2900..3328)
                     /locus_tag="POA77_004707"
     CDS             complement(2900..3328)
                     /locus_tag="POA77_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HDI6368026.1"
                     /translation="MSSPAILQGGYVLPLSRSFTQLLLGIVAQKPPHRHPITGLTINF
                     RDPEYSPEKGGWHPVEIRLIPAGEDWQLDYVTDFHYAGHPWPELEKDVDVSWTQQYTW
                     LSRCGDISHVDAREFWSLWESNFMAYHDMGVFTVRTLWES"
     gene            complement(3376..3807)
                     /locus_tag="POA77_004708"
     CDS             complement(3376..3807)
                     /locus_tag="POA77_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368027.1"
                     /translation="MSKKKIATEAAATESYTVFYSGEAEKISPHSKGLLTYELGKEGE
                     TGSLALRLTANGEGGLFSREWVALDAIHAILEQQQDSFPSRVFRPLFGQGSTNNAGFL
                     AAVLRSPDICLIEADSSRLFMHRCYADWQHRLTQLAAISQD"
     gene            complement(3800..4153)
                     /locus_tag="POA77_004709"
     CDS             complement(3800..4153)
                     /locus_tag="POA77_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368028.1"
                     /translation="MFKLTTPTSLPLLKLPASALEALSNEILGPVDDIDLFTAFWNET
                     ETLLWHLNHDDTLPEDPLLAVALANPEYVTALDDGWYLLLGIVCDNGQGIYLVFPDTT
                     IITQLQNLIEALNHE"
BASE COUNT         1060 a         1063 c         1202 g         1042 t
ORIGIN      
        1 attcgtctgt ttactcccgt aatgcattat ctgatgaata atgttcatgt ggggtaaggc
       61 ttccataaag tcagactgtt caatcagcga aatgagtgtg ttatcgatct caaacgttgt
      121 atccggtaat acctcaacca gcctgattcg ggacagggaa ataacgaaca cgcccgatga
      181 cgtacatccg gcaagaaacc actccgcatg actgcaaagc agacggtagg gatgaacggt
      241 atggctgacg ttttcgtcgg ttctgatacg cacctgggtc tggttgagta tggcgagcgt
      301 cagtctgctg aaatcaccga aaagggtggg tttctggcgc ggctccggta gcataatcct
      361 gtatggcgag ggacgttccc cgtcaagcat caggctcagc agtttggcat ccatcgcggg
      421 taacagacgg tcaagatgca gcaacctcgc cagtatcttc atatcccgtt ccgtgcggcg
      481 acgaaagtaa tgtgaggcaa gctgatacac gccgttgctc tgttcaatat caaggtacgt
      541 cagacggacg ttgaaatccc gtcgcagcgt tcgcgtcgtc actccaaatt cttctgccag
      601 cgtcccgata tgcagctttt caccctgaaa caaacggctc aggatgatgg aaaggcgggt
      661 tgccagtctg tcatgttcgt tttcttgtgt tatcagcgtc atacatcatt tccgaattta
      721 gccgggaaat aatgccagca gtagcggaca ggatttgtcc gccctctgaa gttttttaaa
      781 gtctgaatac cttaaatgta agtgctaaac gtcagtattc tcaggtgaat aaagatgctt
      841 ctggcgcatg taggcaataa aaacgcagga tggtagcgtc acccggtatg cgggaccggc
      901 taaatctatc agtagccaga gagaactgag cacccagccc acgggaccgg caaaagcgcc
      961 catagtccgt gtcagcatgg catttcctgc aacggtcagc cctctgccta acagggcttt
     1021 tgctacgcca tttgtgacaa caagcgccat ctgataggcc gcaaacccgg atgtttttat
     1081 ccccgcctgg attgccatgg tcagcagctc cggtgtggga ttgctgtagc ctgtctgcaa
     1141 ttcttccgcc acagtcttta gctggtcggg cgtcatcttc tcaagacttt tctccagaat
     1201 cttcatcagc agattcattt caatggtttc aacactggat ttgctgttgt agttcacctt
     1261 cattttatcg caggcatcgg taagaatttc ccggtacagc acccctttac cccagcggag
     1321 cagcgtggca aagctgttcg ccccaaacgt ctgaacctcg gcggcaatgg catcccagta
     1381 gcgcgggtgg ttgggcttgt gttttttata ctgaggatca taggtcagcg tttccgtgat
     1441 gcgtgccagt ccgtctttcg gatcgcagat aagcacagaa accagtaaat ccagatcgtc
     1501 acgatgacag tggcgcagaa actcaaggtc ggggtcgtgt cggtaggtag ccatatttca
     1561 cctcattgca gatattggac ctgacagttt aaggaagccc ccggacaggg tttgtccgcc
     1621 tgaaaaacaa aaaagtaaaa aaaaacctgc ccgaaagcag gtttctgttc accggatgga
     1681 ggtataacgc gtcgggatta ctttccgcgc cattcctttt ccactttatc cgcaaaggca
     1741 tcgcactgcc gggcatattc cgggttatag agcagctccg actgcgaaag ctgcatggcg
     1801 gtcatgctgc cgttacgggc gttcgattcg agccaggttc tgccataggt ttggttcatc
     1861 acctgaagta ccctgttgaa taccggtccg ttgatgcggg cattgagatt gacgcctttt
     1921 aactgaaggc cgctgtcgct ggtctgggtc aggcgggtgg agtacatcgc gcagaaggtc
     1981 gaattggcaa tcatctgagc gtcagcctgc tcgtcggcga acgcgctgtg gctgaaggcg
     2041 gaaagcaaaa ttgtggcggt taacagttta aaaattttca tcatgttccc ttatggattg
     2101 aaaagcggag acagcctgcc atacggagtg gtcaggagat gtccggcaga gaaaaaagtc
     2161 agggtttcag cgagggagcc agtcgccagc ggtgaggtcc agcggattcg agcacaaagg
     2221 ccagatgggt gaggtcccgt cttgcggtac ggggagatac gtcaaactgc cggatgatgt
     2281 agcgaacaga tattgtctga ccgtaaaaca gcgccatggt cagccggata agccgttctc
     2341 cctgtccggt gacacgttgc ctgcaacggc tggctttcat cgttgccata cgcgccatcg
     2401 gtcccctttc tgaaaagaat cgaggtgtct ggcaagccag atatcagggc acgaatcgcc
     2461 ggtaacgcag cgggcagtaa accggtggca gttaaagtcg agcaggctat aaggatagcg
     2521 ctgacccact gccgcctttg cccgttcagc ggcccgcgca caaccctgag gcgtcaaatc
     2581 catacagtcg gtatacaccg agatagcgga gttccagcca tcgagccggt caagaaaacc
     2641 tttagcctta cttttgataa cggttccatc gccatcaaga tgaacaatat cgccgttacc
     2701 cacatagata ccgctgtgtg cggcaaggcc aaaaagcagt tcgcactgca cgataccgcc
     2761 gtaagccggt tcatcgacgc aggagagaat gcagttatcg ataaatgaat ccacgaagtt
     2821 cagcagcaga agccggtaca taacattcag ttccttacta cgggggagag gggggatgcg
     2881 ggggaaacgt tgtgaacact cagctttccc acaacgtacg gaccgtgaaa acccccatat
     2941 cgtggtaagc cataaagtta ctttcccata gcgaccagaa ctcacgggcg tcgacatgag
     3001 agatatcgcc acaacgggaa agccacgtgt attgttgcgt ccagctcaca tcgacatctt
     3061 tttcaagctc aggccatgga tgtcctgcat agtgaaaatc ggtgacgtaa tcgagctgcc
     3121 agtcttctcc ggcagggatg aggcgtattt cgaccggatg ccagccgccc ttttccggtg
     3181 agtattcagg gtcacggaag ttaatggtta atccggtgat ggggtgacga tgaggcggtt
     3241 tttgcgcaac gatacccaga agcagttgag tgaacgaccg ggagagcggc agcacatagc
     3301 cgccctgcaa tatagcgggt gatgacatag tgaatttcct ttttaagggg tatggaacac
     3361 agaaaaagcg ggggattagt cctggctgat tgcggcaagc tgcgtcagac ggtgctgcca
     3421 gtcggcgtag cagcggtgca taaagagtct gctgctgtcc gcttcgataa ggcagatatc
     3481 cggacttctg agcacagcag caagaaaccc ggcgttgttg gtgctgccct gaccaaacag
     3541 tgggcgaaat acacgggacg gaaagctgtc ctgctgctgt tccagaatgg cgtgaatcgc
     3601 atcaagcgct acccattcac gggagaacag gcctccttcg ccattggcgg ttaagcgcag
     3661 ggcaaggctt ccggtttcac cctctttacc cagctcatac gtcagcagtc ccttgctatg
     3721 ggggctgatt ttttctgctt ccccgctgta gaacacggta tagctttcgg tggcggcggc
     3781 ttcggtagcg atttttttct tactcatggt tcagcgcctc aatcaggttc tggagttgag
     3841 tgataatagt cgtgtccggg aataccagat agataccctg tccgttatca cagacgatac
     3901 cgagaagcag ataccagcca tcatcaagtg cggtgacgta ttccgggtta gccagtgcaa
     3961 ctgccagcag cgggtcttcg ggcagcgtgt catcatggtt gagatgccag agcagggttt
     4021 ccgtttcgtt ccagaaggcg gtaaacaggt cgatatcatc gacggggcca aggatttcat
     4081 tgctgagggc ttccagcgcg gaggcgggca gtttgagaag gggaagactg gtgggtgtcg
     4141 tgagcttgaa catgattatc tccataatga attgatttac atggaaataa tatgtgcata
     4201 aaaagaggtt ttttacgttc atagtcgttg gggttggtga gctttgtgaa tgtacaaaat
     4261 caaagcaccc gcaggtgtaa aatgtgatga ttgtcacaat aataaaaatt aatggagtgt
     4321 ggttttgatt gttgaaaggt tgatttaccc ttaagttggg ctgcata
//
LOCUS       DAMZII010000237         4343 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZII010000237 DAMZII010000000
VERSION     DAMZII010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.240"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(18..890)
                     /locus_tag="POA77_004710"
     CDS             complement(18..890)
                     /locus_tag="POA77_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDI6368029.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(877..1401)
                     /locus_tag="POA77_004711"
     CDS             complement(877..1401)
                     /locus_tag="POA77_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6368030.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSELPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(1398..1868)
                     /locus_tag="POA77_004712"
     CDS             complement(1398..1868)
                     /locus_tag="POA77_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6368031.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(1865..2413)
                     /locus_tag="POA77_004713"
     CDS             complement(1865..2413)
                     /locus_tag="POA77_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6368032.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(2388..3140)
                     /locus_tag="POA77_004714"
     CDS             complement(2388..3140)
                     /locus_tag="POA77_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6368033.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPLQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(3160..>4343)
                     /gene="yfcU"
                     /locus_tag="POA77_004715"
     CDS             complement(3160..>4343)
                     /gene="yfcU"
                     /locus_tag="POA77_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDI6368034.1"
                     /translation="MSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRH
                     TYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNS
                     TVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGTSDKHTSVDGYYSHDGSLAQVDLS
                     ANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGTRLLIDADGVADVPVEGNGAAV
                     YTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQSVVQATLTEGAIGYRKFAVISG
                     QKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGNVYLAGVKPGEHMSVFWSGVAH
                     CDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKPVIQEQTQQVTPTNPPVSVSAN
                     Q"
BASE COUNT         1124 a         1102 c          978 g         1139 t
ORIGIN      
        1 cagagcaata ctacgaacta cagttcattc aatacatact gaaaacgaca cgtcgaatca
       61 atcggttgcc cactactggt attcacattc accacaatat cgtgtagtac tttatccgtt
      121 accggatgac gagttggcgt aaccgttacc gttgatttat cacaaccgta agaggtaaag
      181 gttaacaatt ctgataccgc gcggcagtat gctcctctgc catcattatc agaaaaatta
      241 ctgccatcac agagtctgcc gccttccatc aagtaggtcg aaagcgtggt ggtcactgtt
      301 gaccccgtgg actgaattcg taacgcctta ttatggctga cataattttc actccaggag
      361 atatcgtatg aagcgaccgg tatcgttggg catattgtgt tatgcactgt aggggccgta
      421 tagcttccaa atccgggtaa atctgtatag gaataaaagg atgcgatcca agggcaacca
      481 taatcatcag atgcaacggt aattttaaac gtcatatatg cacaacgaaa aattttcgta
      541 tctatgccag ggcaatttga gttattaatt gtatcaaagt cagtcagtac caaagttgga
      601 gctgaacccg acccagacca ggaaataacc cgcaacgtat cgcctttata cgcaaaagaa
      661 tcatatttcc catatgcaac ctcctttggc gttatcaccg taacattgaa aacactgctc
      721 gctacagcac cggttacctt gtccatggca taataaactc tcacactgtc aaaactcgca
      781 ctcataagtt tatctccagc ataactctta gatgcgccga caacactcag aacgagcagc
      841 attaggctcg cgaaaattat ggtccatttt ttcatttcag aaatactcaa aggttgccgt
      901 cactgtggca ctaaagtctc cggacgttac atctcgcccg ctctttgcct gtaaccaggt
      961 attaaaattt aggctgttat ttccctgatt cagcgggagc gtgacgcctg ttgtattgtt
     1021 gattggcacc gatgtgcctg ccgcagtttc aataccaatc ccagcgccgg atgctgatga
     1081 cgaggagtca aaggccagaa agccgggtag ttcactatct tctgttcctg tcaacgttac
     1141 tttgacatta aacaatgtag aactatgaca atctttcaat tgaaacacca atggcactcg
     1201 ctctgactgc ccaaggttca ttaaatcgct ggcggcaatg gcaggaaaat gtacttctgc
     1261 caactggcta ccattaatca ctggcgtaca cgatttactg attaaattgc cggtaaagtg
     1321 caaattatct cctgctattg tggttccact gcccagcaac atcaggctac taataaaaaa
     1381 caacgtacgc tttttcttca ttggtaatct acctgtaacg tggcccaggc ctcaaagtcc
     1441 gcctctggca gaacgacacc actctttttt actggcacag ccgttaacac cggtaaatcg
     1501 gtttcattca ccacaagtgg cgtattgatc gtaaaactct gccctgcctg tttgagctga
     1561 atgcctaaac cagtgatatt gctgcttact gcagcattat caaaatcgct tggtgtgcca
     1621 ctccagctca aggtcatttc cattaatggg tcgcgaaccg ccgtatcgca ggtaatggaa
     1681 tacggcacgg tttgctcata attgctgcca ttaatgtcat caataagcac atcctgaaaa
     1741 gttatttcaa tggtttgagc gttgctaatc gtgcagttag gtggctgcac taaataaccg
     1801 tgaaaagaga cgtttgagag tgccatcgag caaaagcccc acagcaaaat aagcgagatc
     1861 cgtttcattg gtaatccacc acaattgtgg cactggcgtt aaattcagct gccgctaacg
     1921 ttgtaccact ctgtttcacc ggcacagctt ccagaacggg aactcccgag gaaaaattaa
     1981 atggcaacca tgagccagag gttaagtcga gtattgtgtg atcactactt ttctgaaccc
     2041 gaataccaaa cccaggaatc cccgtgctga taactgtttc accgttaatc acgaccgttg
     2101 tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt gttacaaaca agcgagtagt
     2161 caatgggacg tttataatca accccattga tcttattaat gaatacatta ccaaactcaa
     2221 ctgccgcacc atttaccgta caaggaggag gggcatcgac aagcacccgt aaattcatac
     2281 ttactgactt actcgctgca actgcaaagc cgctaccaac acaacatatc agtaacccag
     2341 ttaccgccca gacagaacaa gatcctttca tactggctct cccggcctca gcctttgcgg
     2401 ctctgctctt catcgacctg gcaactgtta ccgttgcaag tgaaaaagag cggcatccga
     2461 gcgccatagt cgttgacata ggtcagtacc ggcacgctac ccatcttcac attcagcggt
     2521 gcagaggttt tcggcgacat cactattggc gagaaaccgg ctgccggatt gccatttttc
     2581 tgtgtgctgg cattactgaa aatgacgtaa tacggcgtcg ggttattgac ggtaaattcc
     2641 tgaccgttgc gggttaaagt aaccttatat tgcaaggggt ttttcatgct gacgttctcc
     2701 agcgccttcg gtcgccagaa caacttaata cgcgtttgta gtgctatttg cagcgtgttg
     2761 gctttgtttg attttggcgg gatctcacgc acgttgaagt agaacaggct ttcacgatcg
     2821 gcaggcaatt tgttgatatc cggcattccc tgcactttta cctgcccgtt catcattgaa
     2881 tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac tgatcttgtt acccttttca
     2941 tcttcgatcc agctttgggc cagataaggc agtttcggat cgttattgcg caacgtcacg
     3001 ctgattgatt tgtcgctttc attaaatatc aaacgggtac gatctggcgt tacagaagcc
     3061 tgggcagaca aactggcagc gctaagcagt aacgctagcg tcgtagcacc caatttggca
     3121 gagcaaagta aatcagacat ggatcattcc ttaatcacgt tattggttag ctgaaacaga
     3181 aacaggggga ttggtgggtg tcacctgttg cgtctgctcc tgaatgacag gctttatgtc
     3241 atcaggaaca accggcgcga cgttgccttt atgctggcat ggcagtaaca agccgttaaa
     3301 cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa tgcgcaacac cactccagaa
     3361 cacactcatg tgttcgccag gtttcacccc agccagataa acattgccgt catcatcgac
     3421 aaggcccact gtctgctcgt tatcattttt tacttctgcg ccaaacggag gatggctgcc
     3481 gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg atgacggcaa atttgcggta
     3541 gccaatcgcc ccttcagtta gcgtggcttg taccaccgac tgggttgctt cggcgttttc
     3601 cggcagtttg ttgaggtcga tatacgcctg attgcggtaa tagttattga cgtcagaaac
     3661 gacggcttta ccaaacatat tggtataaac agcagcaccg ttaccttcta ccggaacatc
     3721 ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc atattctggg tacggtgaag
     3781 tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac gacaagcccg cagaggtgta
     3841 ttgcccttca tggtagttcg cactgaggtc aacctgcgcc agcgaaccat catggctgta
     3901 atagccgtca acgctggtgt gtttgtcact ggtgccgacg ttcaactgat agtgagtcgc
     3961 gtcatcgaca cggctgaaat aaccgacctg actgctgtcc gtcccactgc catagttacc
     4021 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt
     4081 gtcggcctgg ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat
     4141 actgcccata ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc
     4201 ccagtaggta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
     4261 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
     4321 ggcatccaga tactcgctca tgg
//
LOCUS       DAMZII010000238         4229 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZII010000238 DAMZII010000000
VERSION     DAMZII010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.241"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="POA77_004716"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="POA77_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6368035.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="POA77_004717"
     tRNA            complement(324..399)
                     /locus_tag="POA77_004717"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="POA77_004718"
     tRNA            complement(406..480)
                     /locus_tag="POA77_004718"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="POA77_004719"
     tRNA            complement(597..681)
                     /locus_tag="POA77_004719"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="POA77_004720"
     tRNA            complement(690..765)
                     /locus_tag="POA77_004720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="POA77_004721"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="POA77_004721"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDI6368036.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="POA77_004722"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="POA77_004722"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDI6368037.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="POA77_004723"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="POA77_004723"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI6368038.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHYVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1096 a         1048 c          942 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctatcttatt tttccgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatccc caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgc tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtatgagca tggccgccaa cgtattacga tcgagattga ttcccgcttc ctgcagcgtg
     2401 atccaccctt gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggca
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtta gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atcagcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataacgggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc gggacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcaccag agtgaaattc gccattcgct aacagcgcga ttaatttcag cggaacggta
     3061 ttatccttca tgaaatcgtc tccactgcgc taacttcacc tgatgcacca ataaagcgaa
     3121 cttcgggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca taatgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gaggactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatctgcct ggggataatt tggtgctgtt ggaaattgtg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcacct
     3661 gcttgcctgt cgccagttca acacagtcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtaccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tttgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 cggggggatg gcttgtagat atgatgacag gaagagtttg tagaaacaca aaaaggccca
     4201 gtcttccgac tgagccttct gcttaattt
//
LOCUS       DAMZII010000239         4228 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZII010000239 DAMZII010000000
VERSION     DAMZII010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.242"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            14..775
                     /gene="ydeO"
                     /locus_tag="POA77_004724"
     CDS             14..775
                     /gene="ydeO"
                     /locus_tag="POA77_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDI6368039.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
     gene            1179..2861
                     /locus_tag="POA77_004725"
     CDS             1179..2861
                     /locus_tag="POA77_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDI6368040.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2913..4070
                     /locus_tag="POA77_004726"
     CDS             2913..4070
                     /locus_tag="POA77_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI6368041.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4036..4158)
                     /locus_tag="POA77_004727"
     CDS             complement(4036..4158)
                     /locus_tag="POA77_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368042.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         1289 a          881 c          837 g         1221 t
ORIGIN      
        1 ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc
       61 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
      121 tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat ttactgaaaa
      181 taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc
      241 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
      301 caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc
      361 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
      421 cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
      481 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaacttaagc aagagcaaac
      541 gacattctca cagattcttt tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt
      601 agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat
      661 ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
      721 tcaaaggcac acgggtatga atacgggcaa cacgatgaat gctttagcta tttgattatt
      781 tgctaacgag tagtcaacca cgcaccctac gtaaaaatta aaaatggggc agtcattccc
      841 tgccccgcgt tgtttttagg cgatatattt attgaaataa ataagtgaca ttcatcacat
      901 atttatgcat ttgcataacc tgttgcatga ttatttatga tctcaattcg gcattttgtc
      961 agtaaaatgc aataatttat taaatatcaa tagattagtt gtttatcggc gagaaattac
     1021 ttagtagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt tttttagcta
     1081 aatctgctat ctctttatgt gactaacttc acttacaccc acttatttct cttcgtaaaa
     1141 ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa
     1201 gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc
     1261 atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc tcagacttta
     1321 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
     1381 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
     1441 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
     1501 caacgccgac gctcctttca ttaatggatg aaggcgtacg gtttactaac ggctatgtgg
     1561 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
     1621 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
     1681 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
     1741 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
     1801 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
     1861 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
     1921 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
     1981 gtgccaaaac acttgaccag ccatttatgc tttacctggc ttataatgct ccgcacctgc
     2041 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
     2101 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
     2161 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     2221 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     2281 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     2341 attatgacaa gctgatttcc gcaatggatt tctacccgac agctcttgat gcagccgata
     2401 tcagcattcc aaaagacctt aagctggatg gcgtttcctt gctgccctgg ttgcaagata
     2461 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     2521 acgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     2581 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     2641 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     2701 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     2761 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     2821 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     2881 aattttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     2941 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     3001 atgaaaaatg gatggatgac agtactctga aagagttcat caaacaatat atcgcagcgt
     3061 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     3121 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     3181 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     3241 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     3301 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     3361 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat taccctcttg
     3421 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     3481 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     3541 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     3601 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     3661 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     3721 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     3781 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     3841 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     3901 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     3961 tccttttttc aaccatggta ccttatatga acgccatggt ggagttagct aagaacagag
     4021 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     4081 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     4141 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     4201 tcagggcatt agcaataccc ttgattat
//
LOCUS       DAMZII010000240         4210 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZII010000240 DAMZII010000000
VERSION     DAMZII010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.243"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..118
                     /locus_tag="POA77_004728"
     CDS             <1..118
                     /locus_tag="POA77_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6368043.1"
                     /translation="ETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            complement(355..1203)
                     /locus_tag="POA77_004729"
     CDS             complement(355..1203)
                     /locus_tag="POA77_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDI6368044.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(1243..1524)
                     /locus_tag="POA77_004730"
     CDS             complement(1243..1524)
                     /locus_tag="POA77_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6368045.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(1521..1790)
                     /locus_tag="POA77_004731"
     CDS             complement(1521..1790)
                     /locus_tag="POA77_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368046.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(1845..2003)
                     /locus_tag="POA77_004732"
     CDS             complement(1845..2003)
                     /locus_tag="POA77_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368047.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            complement(2699..3730)
                     /locus_tag="POA77_004733"
     CDS             complement(2699..3730)
                     /locus_tag="POA77_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6368048.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKNPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          880 a         1154 c         1074 g         1102 t
ORIGIN      
        1 ggaaaccgtg ctgtctgaac tcgtgtgcgc ctgccatgca cgaaagggga atgtttcctg
       61 cccgttgatc gcgtcactac agggcgaagc aggcctggca aggtcagcta tgccttagcg
      121 tgctttattt tccgttttct gaggtgcccc cagccagata atccttttca agtttttcag
      181 ccgtgtagca cctgccgcct aaaaaaagcc gcatggcctc cctggttttg tctctcacat
      241 atcatccctc attaatgcta atgtacacat acattagcat taacatgctg acagcaacaa
      301 gcggcggatt tacgtctgga taacgtaaat gattgcggta tacaggccag gcggtcagat
      361 tttcagccgt gcctgttcct gcagcagttc cagatgatat gcagcaatag cgtttaccac
      421 ccctgccgtc atccgtgcgt cggcgatcgc tgagtgtgcc tgcccttccc aggtcagtcc
      481 ggcctggctg gcagcacagg ccagtgaaat cgtgccatag cggttggtgg ccccataata
      541 tcctgcagcc agctccatcg cacaatatac cgtcagttct tccagccagt cagccggatc
      601 gctatgtgcg gcagcagtct gtttcagaat gcggatgtcg aaccgggcat taaagataat
      661 tactggtcgg tccccgattg catgacgcag ttgccgcgcc acatctgtcc atgatggtgc
      721 gccgcagaga gcctgctcgc tgataccatg cacagcggcg gcctgcgccc cgatggcgac
      781 cgtgggcttc agcctggttt caaataccac ctgaccggcc gcatcggtca ggccgatttc
      841 cagggcttcc gcggtattat ccagtcctgt cgtttcagta tccagaaaga gcggtgccag
      901 actcagccag tcatatgcct gccgtgccat ccggccactg gtgctgttaa gtctggataa
      961 aacggaaagt cgctggccag ccagctgttg tttactcgtc agagggcgtt tctcacgcat
     1021 cggaacacag tctgcaattc ggtatacagc gaacctgcca ccataagtgt ttttatacca
     1081 cttaaccggc tcagcaccgg gggctggttt cattctgaat tcatcgcgca aacgtccttt
     1141 cgagaaacac ctgtcttcgg caatcgcctg cgcacgtctg gtggccagct catcattatt
     1201 catggttatt gcatctcctt tccttatatc cggacagccc ctctatggcc acttctgtgc
     1261 agtatgcaac acgcgcagga tatacacttt tccccactgg ctgtcgacct cataaaccag
     1321 aacaaaatgc gggtgaataa ccagttcccg ggtaccttcc acacgaccgt tgcggccttt
     1381 atacggctgc ccggccaggc tgctggctga ataaccaatc cgttcgtcca gttcaatggc
     1441 agccatcagg ttctgctccg caatgtactc acgaatgcgc ttcctgtcct ggctggccag
     1501 catggtccag aatatctcca tcatttaccg gcaaccttcc tgctcatttc atcccgccat
     1561 gctgcagcag aggattcgac catgtcatgg ggaagcacat cgccgtgctc gagttgctga
     1621 cgtcctgccg caacctggtc acggaaccag gtattatgct gccgttcaac ggtctggcgc
     1681 ataaaatccc ggattaactg agagccatta cggtccatgc tttttgcagc ggccataaaa
     1741 gcatccttca gttcagcgtc gattcttaaa ctcatattaa cctgtgccat ttatcacctc
     1801 gtcattacta cgtatataca cagtatataa cgattcagat atcgtcacca ggatatgccg
     1861 cgccagcggc atggaaggcg gcacgctgtg gttacatgtg ataccggagt aaaaccgccg
     1921 aagcccggcg tcagccggta ctgattgata gatttcacct tacccatccc cagccctgct
     1981 aagccatacc cgctttcagc catgagagag tttctgtgcg cggtcggagt ggtcccgacg
     2041 agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg gccgtcaggg ccgcttacga
     2101 gcgtgtactg agaacatcca gccagaagac tgacagcgat gataatgtag ttacaacatt
     2161 cataattaaa agcgatgttg ttcccgccct ttgggcgggg gcggcctcgc ttttcagtta
     2221 tgaggggtgg gcacggtgct gcgtttgtgt ggtgttttta ttcctgatgt ctttttgttg
     2281 tggtttcaga acggtgaggt tcggggcccg cgcgttagcg tgggaggtgc ttttagattc
     2341 tgagcggcgc ggccttggcc gctgcaagcg aaggcctctt tgtatttgtt cacagtggtc
     2401 tcagagataa agattttccc tgcggcgaag caacgcaggg gattaaataa tggggttaag
     2461 actatattgc aggtttgctg tttcaggttt gcagcttctt aaagttcttt gtttgcggat
     2521 ttatgtggat aactgaaaca acgtggccat gttctgatga ctgatgcgtg gttttatggg
     2581 cagatatgcc cctctgaggg gatttaaggt gattactgcg gattttgggc tgagcggaac
     2641 cagcgccgaa agccccggag gggctggtga tgattcagtg ctgagggaat ggtgctgtct
     2701 agtgcggtgg cggggcagcc agcgtcagtt caagctgacg caactccctg atggccattc
     2761 gcttcagatg gtcatcggaa caaaaatagg cttcatccgg aggcagacgc ttcctgagat
     2821 actctgccat ctcatgaatc tgctggtcgg caggcagttt cttcaggcgt ctggcgcgct
     2881 tactggctgc tgctttcgca cgacggtgtt tcagcgcatc ctgactgcgc tgcagatacc
     2941 agcgttttct ggccgcatgt acggagatat cttcatcctc acgcagaaca ccttcccgaa
     3001 tgagctgctg gcggatttca ctttcacgca gtcgtttctg ctgctgttcg tgaagtttta
     3061 ccatgtcgac gcccagcatc tgccagcctg ccggtgttat ccagacgtaa cgtggcagac
     3121 gctggcgggt ttcacggtcc cacagggttt cctctgaaac acccagcaca ccaaaacgta
     3181 cctgttcggc cagcaaacgg gaaaggcggg agaccgtcac ttccagttcc ggaataacct
     3241 tccccttgct gtctttcggg ctgatttctc tggccagacg ggaaacactc atgcctacgg
     3301 tatgtgtgcc cgcatcactg aagctgacca gaaccggcca gacggcatcg aggaggcgtt
     3361 gtttttccgg acggaaagca tatttgcgac cgacatacgt tcgtttctgc acgtaaaaag
     3421 gatgcagaga catgtgcatg cgcaggctct gctcgccggt caccgggttc tttttcacca
     3481 gacggttgta ggcgtgcccg agctgcccgc tcagcgcctt atagcgctca ggacgatacc
     3541 aggacggatt ttcgcatttg ggtttggtgg agtgctcacc acgacgacgc ttcgtgcgct
     3601 tcctttccgg ttcaaccagg aaagtggtcg cccttcccgc ttcatagtct tcccagaaac
     3661 ggatctgctc ttcgggtgct aactgactcc agtgtactgc gttatatgga gtattcttca
     3721 gacctgccac aagctgtcgc ttatggcgga gcgattgtgc cgtgtattta tacactgcac
     3781 agggttgaat ctctggtacg gtttcattat actttacccg tattctgtga ggcccccact
     3841 atctttcgtc gttccgccaa gttcgtaaga aagatagtgg gggttttcct tttatggctt
     3901 ccggaacatc ttcagtcttc tgacttactg cccgtacatg ccatctctcc tgaatgcctt
     3961 atggcgcatt ctgccgtccg gctttcgccg ctttgctcca cagagtgcca ccctctgcac
     4021 agctaataac agccgaaata tacccgcttt acgttaattt gaaaagcatt acaacgatct
     4081 cttctcttcc agtgcctcct cactgatacc tctaataagt taatcttgac ctgcgtcaaa
     4141 aaaataaaat ctatttttaa aagcagattt ttctgcttta gatctttctt ttggattaaa
     4201 agatcgactt
//
LOCUS       DAMZII010000241         4204 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZII010000241 DAMZII010000000
VERSION     DAMZII010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.244"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            312..920
                     /locus_tag="POA77_004734"
     CDS             312..920
                     /locus_tag="POA77_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDI6368049.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1018..2409
                     /gene="selA"
                     /locus_tag="POA77_004735"
     CDS             1018..2409
                     /gene="selA"
                     /locus_tag="POA77_004735"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDI6368050.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2406..>4204
                     /gene="selB"
                     /locus_tag="POA77_004736"
     CDS             2406..>4204
                     /gene="selB"
                     /locus_tag="POA77_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDI6368051.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDSRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT          938 a         1077 c         1235 g          954 t
ORIGIN      
        1 atgcgcaccc cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcgccctga
       61 cgcgctgccg gttttccgct catctgctca tccttgtttt atcgggttat ttgtctgctt
      121 ccaggcaaaa ccctacagat ttataagaaa gccttaagtg agatatccat cagatagaaa
      181 caattaggaa aatatttata tcaccatgat aaatatgtga tttatttgga agtaacaaac
      241 catcaacatt ttctgccctt tttcccatca ccaggccata ctgatatttt tcctcgcaca
      301 aaggactcgg catgaaactc gtcggtagct acaccagccc gtttgtacgc aaactttcta
      361 ttctgttgtt agaaaagggc ataactttcg aatttattaa tgaactgccc tataacgcgg
      421 acaacggcgt ggcgcaattt aacccgttag gaaaagtgcc ggtgctggtg accgaagagg
      481 gcgaatgttg gtttgattcg ccgatcatcg ctgaatatat tgaattaatg aatgtcgctc
      541 cggcgatgtt gccgcgcgat ccgctggagt cgttgcgggt gcgcaaaatt gaggcactgg
      601 cggatggcat tatggatgcc gggctggtat cggtgcgtga acaggcgcgt ccagcggcgc
      661 agcagtctga agatgaattg ttacgccagc gggagaaaat caaccgcagt ctggatgtgc
      721 tggaaggata tctggtcgat ggcacactca aaaccgatac ggtcaatctg gcgactatcg
      781 ccattgcctg tgccgtcgga tatctcaatt tccgccgcgt tgcgccaggt tggtgtgtcg
      841 accgcccaca cttagtcaaa ctggtcgaaa acctgtttag ccgcgaaagt tttgctcgca
      901 ccgaaccgcc aaaggcttga tgcgcgatat gtcctcctga cccatctcac gttacaatcc
      961 gtggttatgt taaacgccct tctccgtgtg agagggcctt gatcagccag gtttcctatg
     1021 acaaccgaaa cgcgttccct ctatagtcaa cttccggcta ttgatcgctt attgcgcgat
     1081 agctccttcc tttctttgcg tgatacttat ggtcacaccc gcgtggtgga attgttgcgt
     1141 cagatgctcg acgaagcgcg agaagtgatt cgtggcagcc agacgctgcc tgcgtggtgt
     1201 gaaaactggg cgcaagaagt cgatgcccgg ttgacgaaag aagcgcagag cgcgctgcgt
     1261 ccggtgatca acctgacggg aaccgtgctg cataccaacc ttggacgagc tttacaggcg
     1321 gaagccgcgg tggaagccgt tgcgcaggct atgcgttcgc cagtgaccct cgagtacgat
     1381 ctggacgacg ccggacgcgg acatcgcgat cgggcgctgg cgcaactgct gtgccgtatt
     1441 acgggggcgg aagatgcctg tatcgtcaat aacaatgcgg cggcggtgtt attgatgttg
     1501 gcggccactg ccagcgggaa agaggtggta gtatctcgcg gcgaattggt ggagattggc
     1561 ggcgcgtttc gtattcccga tgttatgcgt caggcaggtt gcaccctaca cgaagtaggg
     1621 accaccaacc gcacgcacgc gaatgattat cgtcaggcgg tgaatgaaaa taccgcactg
     1681 ttgatgaaag tacataccag taactacagc attcaggggt tcaccaaagc gatagatgaa
     1741 gcggaactgg tggcgctcgg caaagagctg gatgttcccg tagtgactga tttaggcagt
     1801 ggctcgctgg tcgatcttag ccagtacggt ttgccgaaag agccaatgcc gcaggagttg
     1861 attgcggcgg gcgtcagtct ggtgagtttc tccggcgaca agttgttagg cgggccgcag
     1921 gcaggaatta ttgttggtaa aaaagagatg atcgcccgcc tgcaaagcca cccgctgaag
     1981 cgtgcattac gcgcggataa aatgaccctc gcggcgctgg aagccacgtt gcgtctttat
     2041 ttacaccctg aagctctgag tgaaaaatta ccgaccctgc gcctgcttac ccgcagcgca
     2101 gaggtcattc aaatccaggc acaacgttta caggctcccc ttgccgcaca ttacggcgcg
     2161 gagtttgcgg tacaggttat gccatgtctt tcgcagattg gcagtggttc gctgccggtt
     2221 gatcgcctgc cgagcgcggc attaacgttt acaccccatg atggacgcgg tagccacctt
     2281 gagtcattag ccgcccgctg gcgtgaattg ccagtgccgg tgattggtcg tatttatgac
     2341 ggacgattgt ggctggattt acgctgcctt gaagatgagc aacggttttt ggagatgttg
     2401 ttgaaatgat tattgcgact gccggacacg ttgaccacgg caaaacaacc ttattgcagg
     2461 cgattactgg cgtaaatgct gaccgtctgc cggaagaaaa aaagcgcggc atgaccatcg
     2521 atctcggcta tgcctactgg ccgcagccgg atagtcgcgt gcctggtttt atcgacgtac
     2581 ccggtcatga aaagtttctt tccaacatgc tggcgggcgt tggtggtatc gatcacgcgc
     2641 tgttggtggt ggcgtgcgat gacggcgtga tggcgcagac ccgtgagcat ctggcgattt
     2701 tgcagctgac cggtaacccg atgctgacag tggcgctgac caaggccgat cgcgtggacg
     2761 aagcgcgtgt tgatgaggtt gaacgccagg taaaggaggt gctgcgggaa tacggttttg
     2821 ctgaggcaaa actgtttatc accgcagcaa ccgaaggtcg gggaattgat gccctgcgcg
     2881 agcatctgct tcagttacca ggacgtgaac acgccagcca acatagtttc cgcctcgcga
     2941 tcgaccgcgc atttaccgta aaaggtgccg ggctggtcgt caccggtacg gcgttaagcg
     3001 gggaagtgaa ggtaggcgat tcactctggc tgactggtgt aaataaaccg atgcgtgtac
     3061 gtgcgctgca tgcacaaaac cagccaacag aaaccgccca tgccgggcag cgtatcgcgc
     3121 ttaacatcgc gggtgatgcg gaaaaagagc agattaaccg tggcgactgg ctgcttgccg
     3181 atgcgccgcc agagccgttc acacgggtga ttgtcgagct tcaaacccat acgccgctga
     3241 cccagtggca gccgctgcat attcaccacg ccgccagcca cgttacggga cgcgtttcgc
     3301 tgctggaaga taaccttgcc gagctggtgt tcgacacgcc gctgtggctg gcggataacg
     3361 atcgcttagt gctgcgcgat atctcggccc gcaacacgct ggcgggggcg cgtgtggtga
     3421 tgcttaaccc gccgcgtcgc ggtaaacgta agccggaata tctgcaatgg ctggcgtctc
     3481 ttgcacgggc gcagagcgat gccgatgcgt tatctgttca tctggaacgc ggcgcggtta
     3541 accttgcgga tttcgcctgg gcgcgccagc tcaacggcga agggatgcgc gaattgctgc
     3601 aacagcctgg ctatattcag gctggttata gcttgttgaa tgcgccggtt gccgctcgct
     3661 ggcaacggaa aattctcgac acattagcga cttatcatga gcaacatcgc gatgaacctg
     3721 ggcctgggcg cgaacgtctg cgacgtatgg cgttgccaat ggaagatgaa gcgctagtac
     3781 tgttgctgat tgaaaagatg cgcgaaagcg gcgacatcct cagtcatcac ggttggctgc
     3841 atctgccgga tcacaaagcg ggcttcagcg aagagcagca ggccatctgg caaaaagcag
     3901 agccgctgtt tggtgacgag ccgtggtggg tgcgtgatct ggcaaaagag acgggtaccg
     3961 acgagcaggc aatgcgcctg acgctacgcc aggcggcgca gcaaggaata attaccgcga
     4021 tcgtcaaaga tcgttattac cgtaacgatc ggattgtcga atttgccaat atgatccgcg
     4081 atctcgatca ggagtgtggt tcaacctgcg cggcggattt ccgcgatcgc ttaggcgtag
     4141 gccgaaagct ggcaattcag attctggaat attttgaccg cattggcttt acgcgtcgtc
     4201 gtgg
//
LOCUS       DAMZII010000242         4093 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZII010000242 DAMZII010000000
VERSION     DAMZII010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.245"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..352
                     /locus_tag="POA77_004737"
     CDS             <1..352
                     /locus_tag="POA77_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HDI6368052.1"
                     /translation="AKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            368..646
                     /locus_tag="POA77_004738"
                     /pseudo
     CDS             368..646
                     /locus_tag="POA77_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            749..1414
                     /locus_tag="POA77_004739"
     CDS             749..1414
                     /locus_tag="POA77_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368053.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSKTVTPDSNLKDACIH
                     NGA"
     gene            1472..1762
                     /locus_tag="POA77_004740"
     CDS             1472..1762
                     /locus_tag="POA77_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368054.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGGA"
     gene            complement(2440..2916)
                     /locus_tag="POA77_004741"
                     /pseudo
     CDS             complement(2440..2916)
                     /locus_tag="POA77_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2911..3944)
                     /locus_tag="POA77_004742"
                     /pseudo
     CDS             complement(2911..3944)
                     /locus_tag="POA77_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1140 a          940 c          963 g         1050 t
ORIGIN      
        1 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga agacggttgt
       61 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc
      121 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa
      181 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa
      241 cctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga
      301 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat aagttggagt
      361 cattacccat gcaggaatcg tcattcatgg taatgttgat atcggtgaga atgtcattat
      421 acggcagaat acaaccattg gtgaaaaggt ttctgacagt agggaaaact atattgttat
      481 tggtgataat gtcgatattg gtgcccatac atgtattatt gggttaaatg tgaaaatcgg
      541 aagtaatgtt aaaatcgggg caatgtcttt tattatggaa gatgtacctg ataactgtac
      601 ttatgtcacc aggaaggaat ccagagtgat aattcgcgaa cagtaatgaa atacatgtta
      661 ttattcttct gttggtgact ttaccgactc agatatatac tatctgtatg tcgtgcatta
      721 tatattaacg atacgcagag gtgtgtatat gggcagtgtt acaaagaaac cgtcacctgt
      781 tgttattatt aagaaaaaaa gacgaattct ttctcatcag gatgatgctg tacaggcagt
      841 tcctgatgat ataaaaccag cgccagtaat ggaatttaaa accgactcat tacctcctga
      901 aataccagcc acagtggccg gttccccgga acaacagaaa aaaacaggac gtaagaagaa
      961 gaaaaaaaag aagaacaata gatggaatca tgagtttatc cgacacagtc tggactgtgt
     1021 taaaaaaaca ttccctcagt tacgggctga agagggggga tatcatgcat ttaaaatagg
     1081 catctcaaag gatgtcatgg cttttatttc ggccagtcct gagtgtggac taactgtaaa
     1141 agaatggaat tgtgctgtaa ggatgattac tggcggttac aaatatctgc agcgtatatc
     1201 agtcgcaggt actccgcgtt atggtctgga tggcaaagtt catggaactg taacggagga
     1261 agaagcgctg tatgcgcagg caaaacttga aaaacacacg cagcgttata acgccaggat
     1321 gaaagagaca gaagatgctc gttgcaacca accatcgaaa actgtcacac cggacagcaa
     1381 tttgaaggac gcctgcattc ataacggggc ataattatgt gaaatgctgt cccgctatgg
     1441 aatcaaaatt gagtaacgac acgaggtttt gatgattact gttattattt ctgaggtgga
     1501 tggctggcgt gaatggaaac accgcgcccg aacaaaggat gcacagacag ccatcatccg
     1561 ggctatgaat aaacattttc cacgatctta caattttatc cctgatgata ttgataatgc
     1621 acctgtatta tttgcagctg taacacgtac accgaacgtg aaaataaccg gtcatatctg
     1681 gaaacctatg tggaacaggg gaatttgctg gaacgtcaaa ggtccgccag taatcatcac
     1741 gctcatccag gggggcgctt gaaattcgga aaataaacca cgttaagact atttggggtt
     1801 ctaagccgaa aacctagaaa attccgtaac caaagccagt aattgacaga tttgcatgac
     1861 gttgaatagg tgacgggtta tgtgacgaaa tctgatgcag aaatcgttgt ttcagtgaca
     1921 gtcactcaat cggtcgttta tgtgacaacc cacgccgtta ctggtcgcgg aaaaatccag
     1981 gtttcggggt ttgaggctca gtggaacaat aaccgccgtt aagcggtcat tttaaatagc
     2041 caaaaatcaa ccagccaatt tacatcgtga atctgcgttc ttggctatct gttttggggg
     2101 gggccaagtt ggctttactc atcctggccg tttgcggaac aaacgacgtt gttgatctca
     2161 acagattgat ttacctgtta ttgaccattc aattgaaccg cttaaccgcc ttttgagttc
     2221 cactactccc caaaccccaa atagctgtct agcgcgaaaa atcactgccc gtaacgtatc
     2281 atatggctgg tacgaataaa ttctcactta ctgcaccata aacgtacacg tcaaaacctt
     2341 ccaaacatgc ttcaaaaatc aacaaattcg ctgtatcgga aatttcggcg tttccggcgt
     2401 acaaccccga ttagatataa atgaattaac gtgtgtggtt tattggtctc tgccttctcc
     2461 agcaatgacg caagactttg acataccatc aaaaaaatga tcggcgagct ccagaattcg
     2521 tggattatga gttgtaataa taacggtagg gcaatgctgc cggatatagg taatgatctt
     2581 tatcgcgagt tcttcgccaa tagaggccgt gggttcatca agtacgataa tctttttctt
     2641 acgggctagg gcgcgggcta tgcataatcg ctggatctct ccaccactca aaccactacc
     2701 cagaggatta atatcccggt ctaggaaagc aaggaaatgt tctccgcagt cctctttaat
     2761 taaccctaat gaattcagga tgcaaaacag ttcatcatcg ggaagaatgt catcggttcc
     2821 atacagaata ttatcacgaa tagttccctg caccagaacg gctttttgag gcacaacagc
     2881 aacgtcctcc aggatagacg cactcaactc tgtgttcttc ggttcaagcc ccaacatccg
     2941 ggcaaactgc cgtgtttcat tagcccggta gcatgaacca ttatccgtca gccactctac
     3001 tggagacgcc ggaagctcgt tgccgaagcg gcgttccacc gctcctggca tgacgtcctg
     3061 tactgtttca ctgtcgaagc cgcccgtagt gaccgcccag tacagtgcct cacggtcaca
     3121 gcagtccagc gcgaacgtga ctcgcagttt ttctccgtta tcacagcgga actcgaaccc
     3181 gtcagagcac catcgttgat tgctttcttt cacggccact ttgccagtat gtgcccgctt
     3241 cgatggcggt acagcggttt ttcgctcaag caacagcgca ttctggcgca tgatccggta
     3301 aacacgtttg gcattgatcg caggcatacc atcaagttct gcctgtctgc gaagcagcgc
     3361 ccatacccga cgataaccat acgtgggcag ctctccgata acatggtgta tacggagaag
     3421 cacatccgta ttatcggagt gacggctgcg gcgaccatct ttccagtcat cggttcgtct
     3481 gagaatgacg tgcaactgcg cacgcgacac ccggagacaa cggctgacta agcttactcc
     3541 ccatccccgg gcaataaggg cgcgtgcgct atccactttt ttgcacgccc atattcaacg
     3601 gcttctttaa ggagttcatt ttccatcgtt tttttgccga gcaggcgctg gagttcttta
     3661 atctgcttca tggcagcagc aagttcagag gcaggaacga cctgctctcc tgcggccaca
     3721 gcagtaagac ttccctcctg gtattgcttg cgccagagaa atagctggct ggctgccaca
     3781 ccgtgttgcc gggctacaag ggagaccgtc attcccggtt caaaactctg ctgaacaata
     3841 gcgatctttt cctgtgtagt acgccgtctg cgtttctccg gtcctaagac atcaatcatc
     3901 tgctctccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatatt
     3961 ggttgtctgg agattcaggg ggccagtcta gcagattatg gtgcgcagtt atacaacagc
     4021 agtaatgagg agtctactat ttctcttgtt atgccagatg caattgaatg gaacggaaag
     4081 aatacgccaa gac
//
LOCUS       DAMZII010000243         4036 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZII010000243 DAMZII010000000
VERSION     DAMZII010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.246"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            27..1685
                     /gene="dpiB"
                     /locus_tag="POA77_004743"
     CDS             27..1685
                     /gene="dpiB"
                     /locus_tag="POA77_004743"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDI6368055.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1654..2334
                     /gene="dpiA"
                     /locus_tag="POA77_004744"
                     /gene_synonym="citB"
     CDS             1654..2334
                     /gene="dpiA"
                     /locus_tag="POA77_004744"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDI6368056.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2375..3760)
                     /gene="dcuC"
                     /locus_tag="POA77_004745"
     CDS             complement(2375..3760)
                     /gene="dcuC"
                     /locus_tag="POA77_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI6368057.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1106 a          949 c         1042 g          939 t
ORIGIN      
        1 gctcgggaca acccgcaagg aaaacaatgt tgcagcttaa cgagaataaa cagtttgcat
       61 ttttccaaag actggcattt ccgctgcgta tctttttgct gattctggtg ttctcaatat
      121 ttgtcattgc agccctggcg caatatttta cggccagttt tgaggactat ttaacgcttc
      181 atgtacgcga catggcaatg aatcaggcga aaattattgc ctccaatgac agtgtcatct
      241 ctgcggtgaa aacgcgtgac tacaaacggc tggcgaccat cgctaacaaa ttacaaagag
      301 ataccgattt tgattatgtg gtgattgggg accggcactc gatccgcctt taccatccta
      361 atccggagaa aattggttat cctatgcagt tcaccaaaca gggcgcgctg gagaaagggg
      421 agagctactt cattaccggg aaagggtcaa tggggatggc gatgcgcgcc aaaacgccaa
      481 tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat tggctacctg gtgagtaaaa
      541 tcgatagctg gcgggctgag tttttattac cgatggcagg cgtgtttgtc gtgctgttag
      601 ggattctgat gttgctgtcg tggttcctgg cagcgcatat ccgtcggcaa atgatgggca
      661 tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga ggcgctgttt agttcggttt
      721 atgaagggct gattgcggtg gatccgcatg gttacattac cgccatcaat cgtaacgcaa
      781 gaaagatgct ggggctgagc tctcccggac ggcaatggtt gggtaaaccc attgctgaag
      841 tggtcaggcc cgccgatttc tttaccgaac agattgatga aaaacgtcag gatgtggtgg
      901 ctaactttaa cggtctgagc gttattgcca atcgggaagc tattcgttca ggtgatgatt
      961 tgctgggggc cattatcagc tttcgtagta aagacgaaat ttccaccctc aatgcgcaac
     1021 tgacgcaaat aaaacaatac gtcgagagcc ttcgtacatt gcgacacgag catctcaatt
     1081 ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta tgatcgcgtg ctggcgatgg
     1141 tgcaggggga gtctcaggcc cagcaacagc ttattgacag cctgcgtgag gcgtttgccg
     1201 atcgccaggt ggcggggctg ctttttggta aagtgcagcg cgcccgggaa ctggggctaa
     1261 aaatgatcat tgtccccggt agccagcttt cgcaactgcc gccaggactg gatagcaccg
     1321 agtttgcagc cattgtgggc aatttacttg ataacgcctt cgaagccagc ctgcgtagcg
     1381 atgaaggaaa caagatcgtt gaattattcc tcagcgatga aggcgatgat gtggtgattg
     1441 aagtcgccga tcagggctgc ggcgttccag agtctctacg agacaaaata tttgagcagg
     1501 gggtcagtac gcgtgctgac gagcccggtg aacatggcat tgggttgtac ttgattgcca
     1561 gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga taatgatccc tgcggtacct
     1621 tgttttcaat ctatattccg aaagtgaaac ctaatgacag ctccattaac cctattgatc
     1681 gttgaggacg aaacgccgct ggcagagatg catgcggaat atattcgtca cattcccgga
     1741 ttcagtcaga tattactggc gggaaacctg gcgcaggccc gaatgatgat cgagcgtttt
     1801 aagccggggc taatcttgct cgataactat cttcctgacg gcagagggat taatttattg
     1861 catgaactgg tgcaggcgca ttatcccggc gacgtggtgt ttaccactgc agccagcgat
     1921 atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg attatctcat taagcccatt
     1981 gcctatgaac ggctggggca aacgctaacc cgtttccgcc agcgtaaaca tatgctggaa
     2041 agtattgata gcgccagcca gaagcaaatt gatgagatgt ttaatgccta tgctcgcggt
     2101 gaacctaaag acgaactgcc gactggcatt gatcccttga cgctaaacgc ggtgcgaaaa
     2161 ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg tggcgcaggc actgaccatc
     2221 agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca gccgccatct gattattgct
     2281 gaaattgttc acggcaaagt tggcagaccg caacgcatat accacagtgg gtgacatgat
     2341 aaagccggag gaaacttgcc tccggcatta ctatttactt gcctgtgacc gctgctgccg
     2401 ttcctggcac catcagctct gtagcaacga taacaatcac caaaccaaca agcaccggta
     2461 ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc catcccggca accgcaacga
     2521 ctacgccaga aacgggcgaa agggtacggc ccaggtttga cgcctgcagc atcgggatag
     2581 tcaaatacgc cgggttaatg ccggaagagt gcgccagttt cgggatcatc tcaacaaacg
     2641 cataaaacgg cgcattgcct gaaccggtcg tgactgccgc cagcatggtc agaatcacca
     2701 ataccagcat caggatgata ctcgccgaac caaacgaggt agcgatagaa atcagacttt
     2761 gaataaagcc gatggtgcta agcccctgag cgaatacccc agcggcaacc agcagcatca
     2821 ccacgttagc aaacgcatct gccatcccgc gataagccac ttccagacca gagaaaactt
     2881 tctgggtatt aaagctgcgg aggaactcca gaatggaggc aatcagcata caaatcacca
     2941 gaatagtgat gatgtgtaat tgcggacccc atttaccgtc aaaaatcagt acaccgatga
     3001 tcggcgtgaa cggcaaaatg gcataaaacg caggagcagt ggtggtgatt tcactgacat
     3061 ctaacatttc atgagagatg tgctcttttt tatccagata acgttgccag aagaagtggg
     3121 cgatcgccat gccgataatt gcagcaattg agataggcag cgtcgttttg aaggcgaagt
     3181 caatcagcga catttcggaa gcttgcgccg ccagcaccac atcccctgaa gtcggtgcga
     3241 gaataatcgc cgccggggag gcacaaatgg cagcagctgc gccacgactg ataccaacgt
     3301 ttaccatcac cggaaatagg gttgccatca gcaaaacacc cagaccggtt gcggaagaga
     3361 cggccagaga catcagacag gcgacaaaat aggcggcaat catcagcagg taaggggagt
     3421 taatatactg caatggtttt gacgccagct tgaccaccat atcattcgcg ccgatatggg
     3481 tcatgtaagc ggcaaatcca cacagcatca taatcatcat gccgaggtcg ccgccgcggc
     3541 tcattagtaa tattttaacg tattcaacga tatccgtggc gctgtagcct gttgaagcct
     3601 ggctggacgg taacactttg tgccccataa tggcactgat aatcagcaat aacaggccac
     3661 cgacaaataa cacaccagtg gcggaatacc ctttaatgat gtagcgagct acacccacaa
     3721 taaccacaac cccaataagg agttcaatga atgtcagcat aatttttcct gtctccaggc
     3781 cccaaagtaa ataataaaaa attcctaaag cttaaggaaa aaatatgccc aataaattgt
     3841 cgatgaatgc tgattaaaat caagaaaaac tgccattaag acattgaagt tgctgttttt
     3901 atacatagat aacaaaacgc atatttagcg gttggcaatt atcacataat aatgtgtgca
     3961 aattgatggt ggattgatgc aaatttgtta attgcaatgg tgttagctgc ggctgtgcgc
     4021 tcaaaaaata atctaa
//
LOCUS       DAMZII010000244         3922 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZII010000244 DAMZII010000000
VERSION     DAMZII010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.247"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            55..1206
                     /gene="yiaY"
                     /locus_tag="POA77_004746"
     CDS             55..1206
                     /gene="yiaY"
                     /locus_tag="POA77_004746"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDI6368058.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1371..2909
                     /gene="aldB"
                     /locus_tag="POA77_004747"
     CDS             1371..2909
                     /gene="aldB"
                     /locus_tag="POA77_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDI6368059.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3128..3271)
                     /locus_tag="POA77_004748"
     CDS             complement(3128..3271)
                     /locus_tag="POA77_004748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368060.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3454..3777
                     /locus_tag="POA77_004749"
     CDS             3454..3777
                     /locus_tag="POA77_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI6368061.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
BASE COUNT          965 a          941 c         1023 g          993 t
ORIGIN      
        1 aatgaaaatc ctcagtaagc tgcccaccct tttttacact ttcaggagtg tgttatggca
       61 tcttcaactt tctttattcc ttctgtgaat gtcatcggcg ctgattcatt gactgatgca
      121 atgaatatga tggcagatta tggatttacc cgtaccttaa ttgtcactga cagtatgtta
      181 acgaaattag gtatggcggg cgatgtgcaa aaagcactgg aagaacgcaa tatttttagc
      241 gttatttatg atggcaccca acctaacccc accacggaaa acgtcgccgc aggtttgaaa
      301 ttacttaaag agaataattg cgatagcgtg atctccttag gcggtggttc tccacacgac
      361 tgcgcaaaag gtattgcgct ggtggcagcc aatggcggcg atattcgcga ttacgaaggc
      421 gttgaccgct ctgcaaaacc gcagctgccg atgatcgcca tcaataccac ggcgggtacg
      481 gcctctgaaa tgacccgttt ctgcatcatc actgacgaag cgcgtcatat caaaatggcg
      541 attgttgata aacatgtcac tccgctgctt tctgtcaatg actcctctct gatgattggt
      601 atgccgaagt cactgaccgc cgcaacgggt atggatgcct taacgcacgc tatcgaagcg
      661 tatgtttcta ttgccgccac gccgatcact gacgcttgtg cactgaaagc cgtgaccatg
      721 attgccgaaa acctgccgtt agccgttgaa gatggcagta atgcgaaagc gcgtgaagca
      781 atggcttatg cccagttcct cgccggtatg gcgttcaata atgcttctct gggttatgtt
      841 catgcgatgg cgcaccagct gggcggtttc tacaacctgc cacacggtgt atgtaacgcc
      901 gttttgctgc cgcatgttca ggtattcaac agcaaagtcg ccgccgcacg tctgcgtgac
      961 tgtgccgctg caatgggcgt gaacgtgaca ggtaaaaacg atgcggaagg tgctgaagcc
     1021 tgcattaacg ccatccgtga actggcgaag aaagtggata tcccggcagg cctacgcgac
     1081 ctgaacgtga aagaagaaga tttcgcggtt ctggcgacta atgccctgaa agatgcctgt
     1141 ggttttacta acccgatcca ggcaactcac gaagaaattg tggcgattta tcgcgcagcg
     1201 atgtaatcat catttccaca acggctggca aattgtcagc cgctttttca actatctctg
     1261 taacccttgc ccgtaaattc gtgatagctg tcgtaaagct gttaccgact ggcgaagatt
     1321 tcgccagtca cgtctaccct tgttatacct cacaccgcaa ggagacgatc atgaccaata
     1381 atcccccttc agcacagatt aagcccggcg agtatggttt ccccctcaag ttaaaagccc
     1441 gctatgacaa ctttattggc ggcgaatggg tagcccctgc cgacggcgag tattaccaga
     1501 atctgacgcc ggtgaccggg cagctgctgt gcgaagtggc gtcttcgggc aaacgagaca
     1561 tcgatctggc gctggatgct gcgcacaaag tgaaagataa atgggcgcac acctcggtgc
     1621 aggatcgcgc ggcgattctg tttaagattg ccgatcgaat ggaacaaaac cttgagctgt
     1681 tagcgacggc tgaaacctgg gataacggca aacccattcg cgaaaccagt gctgccgacg
     1741 tgccgctggc gattgaccat tttcgctatt tcgcctcgtg tattcgggcg caggaaggtg
     1801 ggatcagtga agttgatagc gaaaccgtgg cctatcattt ccatgaaccg ttaggcgtgg
     1861 tggggcagat tatcccgtgg aacttcccgc tgctgatggc gagctggaaa atggctcccg
     1921 cgctggcggc gggcaactgt gtggtgctga aacccgcacg tcttaccccg ctttctgtac
     1981 tgctgctaat ggaaatcgtc ggtgatttac tgccgccggg cgtggtgaac gtggtcaacg
     2041 gcgcaggtgg ggaaattggc gaatatctgg cgacctcgaa acgcatcgcc aaagtggcgt
     2101 ttaccggctc aacggaagtg ggccaacaaa ttatgcaata cgccacgcaa aacattattc
     2161 cggtgacgct ggagctgggc ggcaaatcgc caaatatctt ctttgctgat gtgatggatg
     2221 aagaagatgc ctttttcgat aaagcgctgg aaggctttgc actgtttgcc tttaaccagg
     2281 gtgaagtttg cacctgtccg agtcgtgcct tagtgcagga gtcaatctac gaacgcttta
     2341 tggaacgcgc catccgccgt gtcgaaagca ttcgcagcgg taacccactc gacagcgtga
     2401 cgcaaatggg cgcgcaggtt tctcacgggc aactggaaac catcctcaac tacattgata
     2461 tcggtaaaaa agagggcgca gacgtgctca ccggcgggcg gcgcaagctg ctggaaggtg
     2521 aactgaaaga cggctactac ctcgaaccga cgattctgtt tggtcagaac aatatgcggg
     2581 tgttccagga ggagattttt ggcccggtgc tggcggtgac caccttcaaa acgatggaag
     2641 aagcgctgga gctggcgaac gatacgcaat atggcctggg cgcgggcgtc tggagccgca
     2701 acggtaatct ggcctataag atggggcgcg gcatacaggc tgggcgcgtg tggaccaact
     2761 gttatcacgc ttacccggca catgctgcgt ttggtggcta caaacaatca ggtatcggtc
     2821 gcgaaaccca caagatgatg ctggagcatt accagcaaac caagtgcctg ctggtgagct
     2881 actcggataa accgttgggg ctgttctgat ataagaagct ggtcgcattg ggtattcatt
     2941 gcctgatgcg acgcttacgc gtcttatcat gcctacggga acctgaccgt aggccggata
     3001 aggcgtacac gccgcatccg gcataaacaa agcgcacgtt gttacaattt acgttttaaa
     3061 cctacgtgcg caataaccca gactacgcca acagaagtgc gtttctgggg cgtgttttgt
     3121 caggagttta atctcgcgtt tcgatggtga gcgggcgaac caggccgtaa atcaccattg
     3181 gtgatccgag gccaatcagc aataaacggt agataattga aggtttaata cgcttaatct
     3241 gtactgtgga tgttttactg gagttattca ttagtttggc caatagttcc actatttcaa
     3301 tatgataaac ctcgtcctgt tgaaaaacca gacagcacat ttaagaaagt atataaaaaa
     3361 caatatgtcg ttatataaat aaggaaattg cactgcatca tggcatttca taatatgcgt
     3421 taattgttct tttttatatt ctttgaggga aatatgttcc tggactattt tgcactggga
     3481 gtgcttattt ttgtatttct ggtgatcttc tatgggatca ttattttaca tgatattccc
     3541 tacctgattg ccaaaaaacg taatcatcct catgccgacg ccattcatgt tgctggttgg
     3601 gtgagtcttt ttacgttaca tgttatctgg ccgtttttgt ggatttgggc cacgctttac
     3661 cgcccggagc ggggatgggg aatgcaaagc catgattcat ccgttatgca actgcaacag
     3721 cgcattgccg ggctggagaa acagctcgcc gacatcaaat cctcttctgc cgagtaatac
     3781 ttatggattt actgattatt ttgacctatg tggcttttgc atgggcaatg tttaagatct
     3841 tcaaaattcc cgtaaataaa tggaccattc ccacagcggc cctgggtgga atatttattg
     3901 tcagcggtct aattctgtta at
//
LOCUS       DAMZII010000245         3886 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZII010000245 DAMZII010000000
VERSION     DAMZII010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.248"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            758..1000
                     /locus_tag="POA77_004750"
     CDS             758..1000
                     /locus_tag="POA77_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDI6368062.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1032..1682)
                     /gene="tetR(A)"
                     /locus_tag="POA77_004751"
     CDS             complement(1032..1682)
                     /gene="tetR(A)"
                     /locus_tag="POA77_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDI6368063.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1788..2987
                     /gene="tet(A)"
                     /locus_tag="POA77_004752"
     CDS             1788..2987
                     /gene="tet(A)"
                     /locus_tag="POA77_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDI6368064.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3019..3879)
                     /locus_tag="POA77_004753"
     CDS             complement(3019..3879)
                     /locus_tag="POA77_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI6368065.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          746 a         1219 c         1190 g          731 t
ORIGIN      
        1 caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa
       61 acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca
      121 ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc
      181 atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg
      241 tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg
      301 gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg
      361 gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt
      421 ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt
      481 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
      541 tgagcgtcgg aaaggcgctg gaagccccgt agcgacgcgg agaggggcga gacaagccaa
      601 gggcgcaggc tcgatgcgca gcacgacata gccggttctc gcaaggacga gaatttccct
      661 gcggtgcccc tcaagtgtca atgaaagttt ccaacgcgag ccattcgcga gagccttgag
      721 tccacgctag atctatctca tctgcgcaag gcagaacgtg aagacggccg ccctggacct
      781 cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg
      841 gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
      901 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
      961 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
     1021 agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     1081 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
     1141 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     1201 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     1261 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     1321 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     1381 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     1441 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     1501 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     1561 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     1621 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     1681 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     1741 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     1801 gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     1861 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     1921 atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg
     1981 cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg
     2041 tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc gggcggatcg
     2101 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     2161 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     2221 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
     2281 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     2341 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     2401 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     2461 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     2521 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     2581 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     2641 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     2701 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     2761 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     2821 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     2881 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     2941 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     3001 aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc taggagtgcg
     3061 gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga tgatttgaag
     3121 cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc ccgggctgag
     3181 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
     3241 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
     3301 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
     3361 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
     3421 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
     3481 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
     3541 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
     3601 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
     3661 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
     3721 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
     3781 ccgcagcatc gcgaccgtca tcggtgagaa gttcggcagg tattgggtgg tgacaatgta
     3841 ggtgctgccc caaatggcag gtgctatcgc tgtgaacaat aaatcg
//
LOCUS       DAMZII010000246         3789 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZII010000246 DAMZII010000000
VERSION     DAMZII010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.249"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(331..1170)
                     /locus_tag="POA77_004754"
     CDS             complement(331..1170)
                     /locus_tag="POA77_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368066.1"
                     /translation="MLKLELIILPLLAFLGPSNITNASTKTPIPSSQLSDAKTGVNYE
                     FITIAANYVNGMWLVDGRQRPVIKTSMTKRNYLQIENDSASTPLNLVIPKIEFSIIAK
                     NGVFISKFISLDEDASGKRILWLEPGSSMTISFVNDLSSTPLQALVSVTKQKKEVIAI
                     FGPDQGKKFTLPLPSDSQQSSIDYVPSPEIALRINAPKKLQPAIAIPEDASASLLPSH
                     TYKIRAAEKVRTFKFSSFVWVGKRNNLLGGSDYFTKELKIYPGENFDISTIQKTTMRT
                     END"
     gene            complement(1170..2117)
                     /locus_tag="POA77_004755"
     CDS             complement(1170..2117)
                     /locus_tag="POA77_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368067.1"
                     /translation="MASEHVQTIDLTNFSGRVFFLADPHGHYSTLCNLIHSISEPDEE
                     LIVFSTGNLFDYGPEPMELMTAINTGIFDGRSVRVFSAAGAGEEMLKKLLPINKDRRT
                     YYPSTFLNERWCARGGRWHKQINRNYLEEEICKLLSTQLATVMKVLFKGNISIGICPS
                     DYTDIRQGFNNTYNALLAFNQANVNIFQSQFLYGMDHAVRPVNVSDVNLVVLGRNPVN
                     SIRKAHGLPLTNLPVLIGNCLHINTGSLYMSEISSPALLAPGIPPTTVPAITLVELIL
                     ASTPTLICHQMILNKNGIYTQNTAPLNLDSNDNNIEATL"
     gene            2491..3525
                     /locus_tag="POA77_004756"
     CDS             2491..3525
                     /locus_tag="POA77_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stbA family protein"
                     /protein_id="HDI6368068.1"
                     /translation="MTELAKTKLAVAESNSTELETLKVVIDDGSKAAKLVCTNNQGDL
                     VPLLTQNSFVADFRVSHDGLIPFNYLIDGLQRFSHHSESSNALETTDVAHQYDEISRL
                     NVHHALHSSGLMPQDVHLYVTLPLSQFYTALGETNNENIQRKKDNLMKPVERYIDGKR
                     VSFNVVSVTVFPESLPAVTRADEIESIESFESSLVIDLGGTTLDVASITGQLEQISSV
                     RGFDRIGCSIVYDEIRRYLDSAKLNASNAYIHHLVDNRENKASLKVSPEDLDGVFNAV
                     NGAVAQLQEKVIKAVTQVEERPHNVFLVGGGSYLIEPAVRKHFDKSKIIMVDNPQFAL
                     SLAIADTVFA"
BASE COUNT         1072 a          735 c          841 g         1141 t
ORIGIN      
        1 tcgtaacaaa gaccaagtaa accaactgtg ggcaccgtaa aaattgtaac tgaactaggt
       61 gatatcaact gttttccacg tgacgaatag gtgcaacgac tcccgccgag attcagatca
      121 gacaactcaa taaattcttt ggtcccattc ttggcgagta accagacatc agtgcctgaa
      181 ctctcaatca gaaaagtaac ttttccgtcg gtgtaggctt cagttctgtt gctcattata
      241 gggtcgaacc ctaccgaagg gaggactgca attttgcttc caaccgactg acatccagtc
      301 acaagtaagg taagaataaa tgctgaaact ttaatcattt tcagtcctca tagtcgtttt
      361 ctgtatggta ctgatatcga agttttctcc tgggtaaatt ttcaactctt tggtgaaata
      421 atctgaacca ccgaggaggt tgttacgctt tccaacccaa acgaacgacg aaaatttaaa
      481 agtccttact ttttctgccg ctctgatctt gtaggtatgt gaaggtaata aagatgcact
      541 agcgtcctcc gggattgcga tagcgggttg aagttttttg ggggcattga tacgtaatgc
      601 gatttctgga gaggggacat agtcgattga cgattgttgt gaatctgacg gtaggggcag
      661 ggtgaatttt ttaccctgat ctgggccaaa aatggcaata acctcttttt tctgctttgt
      721 tacggacact aacgcctgta acggggtact ggatagatca ttgacgaatg aaattgtcat
      781 gctggaacca ggttctaacc agagaattcg tttcccggat gcatcttcat caagggagat
      841 aaattttgat ataaacacgc cattttttgc aatgatggaa aattcaattt tgggtattac
      901 aagatttaaa ggagttgaag cagagtcgtt ttcaatctga agatagttac gcttagtcat
      961 gcttgtttta attactggtc tttgtcgacc atcaacaagc cacattccgt tgacgtaatt
     1021 tgcagcaata gtaataaatt catagttaac cccagttttg gcatcagata gctgggacga
     1081 cggtatcggg gttttagtcg atgcatttgt aatgttggat ggacctaaaa atgccagcaa
     1141 cggcaaaatt atcagttcta atttaagcat tataatgtgg cctctatatt attatcattt
     1201 gaatccaggt taagtggtgc agtgttttgt gtgtaaatac cgtttttatt caggatcatt
     1261 tggtggcaaa tcagggtcgg tgtagatgcc aggatcaact cgaccagtgt tattgccgga
     1321 actgtagtag gcggaatacc tggtgccagt agtgctgggc tactgatttc tgacatgtac
     1381 agagaacctg tattgatgtg gaggcagttt cctatgagta ccggtaagtt agtcagcggt
     1441 agcccatggg cctttctgat gctgttaact ggattccgac ccaatactac gaggtttaca
     1501 tcgctgacat tcactggcct gactgcgtga tccatgccgt atagaaactg actctgaaaa
     1561 atattcacat ttgcctgatt aaaagcgagg agggcgttat atgtattgtt gaagccctgc
     1621 cgtatgtctg tgtaatcgga tgggcaaata cctatagaaa tattgccttt aaaaagcacc
     1681 ttcatcacag tagccagctg ggtggagagc agtttgcaga tttcctcttc aagatagttg
     1741 cggttaattt gtttatgcca tcgcccgcct cttgcacacc accgttcatt aagaaatgtg
     1801 cttggataat aagtcctgcg gtctttattt atgggtaaga gctttttcag catctcttct
     1861 ccggccccgg cagctgagaa cacacgaaca gaacggccgt caaaaatccc tgtgttgatc
     1921 gctgtcatta actccatagg ctcaggtcca taatcgaaga gatttcctgt ggagaaaacg
     1981 atcaactcct catcaggttc tgatatcgaa tggataaggt tacatagcgt cgaataatga
     2041 ccatgagggt ctgccagaaa gaaaaccctt cctgaaaaat tagtcagatc aattgtctgg
     2101 acgtgctcgg atgccataaa tgcctctttc tgaaattgca ttaatactac cggatgaaag
     2161 tcatcaattt tttcgataag aggggtaatt tttgtgagtg tccaaaatgg agctagaaat
     2221 catttttcgg tttccgagcc gtatcaactg taatgcacct cagttgtttt atttttggta
     2281 taaaaacgct gagcaaaata tagtttttgc gtgtgttgct caggtgtaca tgaatctagg
     2341 gttgtatctg gattaaaact gaaggattct tttttaacaa ggacttacgt tagtgttttg
     2401 tcccgtttaa tgttgaccta aagcaaccat tggtgtataa attccataac aaaagtggtg
     2461 ggctaaataa accaatacga gggtttctgc atgactgaat tagcaaaaac aaaactggct
     2521 gtggctgaat caaactctac tgaacttgag acactcaaag ttgttattga tgatggtagt
     2581 aaagcggcaa aactggtttg cactaacaat caaggtgacc ttgtaccttt attgactcaa
     2641 aatagtttcg ttgcagattt ccgggtaagc catgacggac ttattccatt caattatcta
     2701 attgatggtc ttcaacgatt ctcgcaccac agcgaatcaa gcaatgcttt agaaactacc
     2761 gacgttgcgc atcagtacga tgaaatcagt cgtcttaatg ttcaccatgc tctacattcc
     2821 tcaggtctaa tgccacagga tgttcatctc tacgtaactt tgccactcag tcagttttat
     2881 accgctctgg gtgaaaccaa taatgaaaac atccaacgaa aaaaagacaa cctgatgaag
     2941 ccggtggagc gttatattga tggtaaacgt gtttcattta atgttgtttc agttaccgtt
     3001 ttcccggaat cccttccagc ggtaacgcgt gctgatgaaa tcgaatcaat tgaatctttt
     3061 gaatcaagcc tagttatcga tttaggtggg acaacacttg atgttgcaag cattacaggt
     3121 cagctggaac agatctccag tgtcaggggt tttgaccgta ttggttgctc aatcgtatat
     3181 gatgaaatcc gtcgttattt ggattctgca aagctgaatg caagtaacgc ctatattcat
     3241 cacctagtcg ataatcgtga aaacaaagcc tcgctcaagg tgtccccgga agatcttgac
     3301 ggcgtgttca atgctgttaa tggggctgtg gcgcaacttc aagaaaaagt gattaaagca
     3361 gtcactcaag tggaagaacg gccgcataat gttttccttg ttggtggagg ttcttacctg
     3421 attgaaccgg cagttcgtaa acattttgac aaatcgaaaa tcatcatggt tgataatccg
     3481 caattcgcac tttcactggc tatcgcagat actgtttttg catgatgatg gttttgcatg
     3541 atgatggttt tgcaaagggg agagttttat gactaaaaaa tctgagaagg aaaacgatcg
     3601 tattcagatt agtgcgtttt ggttatcgga acgacaatct ccgtatgcat ataatttttt
     3661 gaaaaaaaat gccctaacac atcgtggcga gcagatttct ctaataagat ctgcaatcac
     3721 tacggggtta gtgctaaata atctcttccc cgagttatct agttttatta atggactgaa
     3781 tgagagatt
//
LOCUS       DAMZII010000247         3723 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZII010000247 DAMZII010000000
VERSION     DAMZII010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.250"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            168..920
                     /locus_tag="POA77_004757"
     CDS             168..920
                     /locus_tag="POA77_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HDI6368069.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            917..1471
                     /locus_tag="POA77_004758"
     CDS             917..1471
                     /locus_tag="POA77_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HDI6368070.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1473..1745
                     /locus_tag="POA77_004759"
     CDS             1473..1745
                     /locus_tag="POA77_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI6368071.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
     gene            2253..>3723
                     /locus_tag="POA77_004760"
     CDS             2253..>3723
                     /locus_tag="POA77_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368072.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICSDFFDLLVGIKNNKLKIDFESYDKPFHLADIRELLLLMKIL
                     NAPSGDLKLTIKNNEGNETSFLASDNSFSANPITNEIIKAEQIALALIQVASYFRVDK
                     NCKVSINELIRKQKDIERMHTLISKVPGGYPALRLEIPEHDDNIDSSKELRIFIAIPL
                     IFSSFSICLTCVARGSFTYQNNAMTFNDYEINIEKLIRAN"
BASE COUNT         1142 a          775 c          833 g          973 t
ORIGIN      
        1 gtagtgaata gtatgtatta aaggaagcgt gtagatcaga catgacacct ttctctggct
       61 agccagaaca aggtcattgt tcagtcactg acacaatcct cctcggtagt gcgcttgtat
      121 ggaccccggc acaattgaca caacaacaaa acactaccgg agcagtcatg actactgtta
      181 accagatccc tgatgcaaac attacaccct ccctcccacc gaaaattcgc gaagcggtgg
      241 ataaagttaa ggcagcaaaa gctgtctggc aggaagaacg gcgaaaacaa actgaagctg
      301 ctgcaatgat tgaaactatc cgtaaacgtc aggaagatac aaaaacggaa acgcaggcgc
      361 ttaatgatga atggcgaaac ctgtttcgtg agaatcaggg aaatatgacg ccacgcatga
      421 aaaaactgcg ggcagaaatc gccctcggac gcgaaacact ggatgagttc gaagatttgc
      481 tggcagctca ggcggcagaa aatgaattcc tgccctggaa aactgcggat gctgcaaacc
      541 actacatcag cgaacataat cgcctgattg aaactcatgc agtgtggctc tggaatgagt
      601 ttatgaagga acacggtcag aaacttattc agatccttgg actgctgaaa atgactctgg
      661 gccgaagcgc ttctgctgtt atcggtgtag ttcataccgt aaacgacccc gaaagtgtgc
      721 tgaagcaatt tatcagcgag caactcacca ctccggcact gtcctgtaac gtatcttcaa
      781 ccgatgatat taccctgccg gggatcagca tttatgcgga cgataaagcc atccaggatg
      841 ccagacaatc ccccagccct gcagcgcgtt cacggatgct taaacagcgt gacatggtta
      901 aagggggcga gaagaaatga atgccggaac cattactcag gaagcactca acgattaccg
      961 ctcggcgata aaaagctggc tgacactacg taatacgcaa agtacctacc agcttcgtct
     1021 ggcggctctg ctggatactg aagaaaaacc ggcagcatat gccagccagc ttgaggatct
     1081 tcgtgagcgt cttgcagtcc tcgaatggca gattaactgt gctgcacggg atggtcttta
     1141 tgctcatcag attgtgctgg agagttgtgt tacaagcgca acggaaaact tcatgagcga
     1201 gcatggtgat gcactcactg acgctctggc tccttttctt tgcgctccat acgggcttga
     1261 ggcggcaatg aaaatattac gaaccgctgt agcccgacaa acggaaatgc gtactccggt
     1321 aatttcagca gcatataaga gcattatcga cgaaaccgga ttaacggtgg atgcatcaat
     1381 gcgcgctgat gcttcagcca gtttcacccc ggcacaacat aaagttttcc tggcccgcct
     1441 taaccgactt aatgaaaaag gagtgtgctg atatggccct gaagtgtcct gaatgtggcg
     1501 cggtcgctca cgccagaacc agcgcctatg aagctccgtc ggttaaacgc tcatggtatc
     1561 agtgccagaa tctggaatgt tcctgcacat ttaccgcact ggaaagcgtg gatacgataa
     1621 ttatgaagcc tcggtgcaat gaacaggaat cagacaaagc aaaaataccg gaaaaacaac
     1681 agcaaactct caatcgctat ggctccgcgt caaagctgtc aaaccgtcag caaattcctg
     1741 tctgattagc aaaatacgac cacgaagtcc cggtccagta ccgggatttt ttacgccttt
     1801 ttcctggctg gcctgagagc gtatgagtgc atatctatgg cgcatgaaaa cgcatgagtc
     1861 tcatgcgcca tttttgacgc gaaagccctt gtgtggtggc ttctgagacg atttacgggg
     1921 tgcatgaaaa ccagtctgtt aagcgaagcg ggcaggcggg cggggcattg cgcgcaggaa
     1981 tgtgtatacc gttttagtaa tgatcgctga catctcactt ttaataatca tgatggtaat
     2041 gtgattattg tgaatacgag ctcaaggttt gagctccgga aaaaatacgt tgggacgtta
     2101 aaaatcaact cgggagtatc cacagttgat cctcattggc cccctgtaag caagcgcatc
     2161 aagctaatat ttttcagccc gacacttcat agtttttcct gcattggggt aagctaaacc
     2221 tagtatcgta catatgaaag agggatgagt ttatgagtcg aagaaaagtt ggtaatatgg
     2281 gacgtggtga ttttgacagg ctttgcaatg catctggctt gatttacaac agctcctctg
     2341 aggatgattc cggagggtgg gatgccattg tcgaatttcc tctgttaggt ggtatagcct
     2401 tactgaatag tttgaatgaa cagcctatac aatgtttagt gcagataaag tcttctgacg
     2461 gagaaaagaa aggtgtacag gtcaaacttt ctaacatgaa acgtttctgt gacacacctc
     2521 taccctgttt tttctttttt gcgcgttata ataagaatgc taatatcgaa tcagcttacc
     2581 ttgtccacat taatagtgat atcatgtttc aggttctgaa gcggattcgt caaaatgata
     2641 taggaaaaaa atcaccactt cacaaacttt ctttcacggt aaattactct gatgaggatg
     2701 agatagacct gactaacgaa tatgcactca aaaatgctat tgaaaaacat attcctcatg
     2761 gaatgaaaaa atatagtgaa gataaattga gagatttagg taatgtagga tataacgaaa
     2821 gccgctatgc attcaatttc aaagttgata ataatgagga ttataagtct cttattagtg
     2881 ccacgcttgg atatccgacc agaataaatg taaaagatgt tcgcggttgg gatactcgat
     2941 ttgatattaa ttttcagatg gaagaactga cagctgaaca agcaataatc gaaataagat
     3001 ctgttgaagc attctcaaca ggctctataa tattcgaaga tgaaaaagaa gatgtctctt
     3061 ttaagtgtga atttttcgtt tctcctattg cacttaatgc tccaagggat ctggcaagct
     3121 ttagaatttg ctcggacttt ttcgatttac tggttggtat taaaaataac aaattgaaaa
     3181 tagattttga atcatatgat aagccttttc atttagctga cattagagaa ttactgttat
     3241 taatgaagat cttaaatgcc ccaagcggag atctgaagtt aacaataaaa aacaatgagg
     3301 gtaacgagac atccttttta gcctcggata attcattttc tgcgaatcca attactaacg
     3361 aaataataaa ggcagaacag atagccttgg ctttgataca agttgcttca tactttagag
     3421 tagataaaaa ttgtaaagta agtataaatg aattaattcg caaacaaaaa gatattgaaa
     3481 ggatgcatac cttaattagt aaggttccgg gcggctatcc agctcttcgc ttagagattc
     3541 cagaacacga tgataacatt gactcttcaa aggaattaag aatatttatc gccatccctt
     3601 taattttctc atctttttca atttgcctca cttgcgttgc caggggctcc tttacatatc
     3661 aaaataacgc tatgacattt aatgattatg aaatcaatat tgaaaaacta attcgcgcaa
     3721 ata
//
LOCUS       DAMZII010000248         3705 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZII010000248 DAMZII010000000
VERSION     DAMZII010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.251"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            426..989
                     /locus_tag="POA77_004761"
     CDS             426..989
                     /locus_tag="POA77_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368073.1"
                     /translation="MHALSIPPSVARANLATKFSSHLKVIALFNTMQDSQVVVLSSLI
                     EGHQLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYF
                     IEPEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGGVSQAVLSLLHHAY
                     KDKPLSDEMWKEVEDKFTHMLDELSAA"
     gene            complement(1018..1479)
                     /locus_tag="POA77_004762"
     CDS             complement(1018..1479)
                     /locus_tag="POA77_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368074.1"
                     /translation="MSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVID
                     LKGLVFGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIQKEEYVSRAYKIPTPEFI
                     HINDNKEKIQEDFDKFVNRYIEAHVKNDENILRNYRYSTLKNYHKELGLED"
     gene            3462..>3705
                     /locus_tag="POA77_004763"
     CDS             3462..>3705
                     /locus_tag="POA77_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368075.1"
                     /translation="MAFISLSNLAAKTGRIIQSQYVTATQTKKSTGAVELGFRLSKSA
                     MDETGITIGDRVDILYDKEENQWMIKKISEGGFGVSG"
BASE COUNT          950 a          847 c          851 g         1057 t
ORIGIN      
        1 aaaaaaacaa gcacggcggt tactcatcgt gtgctgttag attgaccctg cgacctggtt
       61 ttcctcgcct ttctgaggat gaagaggaca acaagaagct actttctgat gattcattca
      121 ctcagctaag tgaaggtgta atcatcttca agctgggtga tgaataggaa ggaccaaaaa
      181 ggaaaggcag agttagtgaa cgtcgccaaa caatcgacta actccgcctt ctggtcaaca
      241 catcactacc tttggaaggg tggagtgtta tgggcggcat tatgttcata aacttgcatt
      301 ctaataaaga acaaaatcgt ttcttttgag gaatgtgaac acgacatttt ctattctgat
      361 aggtgcgatg ctattggctt tggtctctta agtttatgtt tttaaacata tatgaggcta
      421 tacatatgca cgctttatcg ataccacctt cagttgcccg cgctaacctg gcaactaaat
      481 tctcgtctca tttgaaggtg atcgcgctct tcaacacgat gcaggattcc caagttgttg
      541 tcttgtcatc actgatcgaa ggccatcaac tcaccagttc tggtaacagt gtcaaagccg
      601 actttgaagt cactcgctta ccggcgatca tcgagatgtt agagaaaaaa tattttttcc
      661 ctattcgtca tctaaatgta tctgttaaat cagtaactac aggaaggatg actggacaaa
      721 ctgtctattt catagagcct gaacacatcg agcaactcct cgctgatcct gagcttgtgt
      781 ttgctaatca ggaaaggagc ctcttctttc gttctctcga aaaagaggga aaaaatcttg
      841 gcaagcttat cgagaaaaaa ggtggtgttt ctcaagccgt cttaagcctt ctacatcacg
      901 cctacaaaga taaacctctt tctgacgaga tgtggaagga ggtagaagat aagttcacac
      961 atatgctgga tgaattgagc gcggcataaa ggcaagggcg gtcattagac cgccttttta
     1021 gtcttctaaa cctaattctt tgtgatagtt tttcagcgta gagtaacgat agtttctcag
     1081 tatgttttca tcgttcttga cgtgcgcttc tatgtatctg tttacaaatt tgtcgaaatc
     1141 ttcttggatt ttttctttgt tatcattgat atgtataaat tcaggagtcg gtatcttata
     1201 ggctctagat acatactctt ctttctgaat tagcaacgct ttggtgtaat ccagtccttt
     1261 cttaacacct tctgagcgtt ccgaaacgaa cccaaattta tggtttaaat ggcttcgtaa
     1321 gggtatcccg aacaccaatc ctttgagatc aataactacg attccgtagc ctcttacttt
     1381 tccatcatta ctgccgtcta gagcctcttg taggcctttg ttatcagcgt aaaactggtc
     1441 agtaagggtt cttaactgta tgtaatcctg tttgctcatt tttgatgtcc attggcattt
     1501 tcctgtaacc aatgtacaaa aaaagcaaag ccctctgtaa gagagggctt tgcttttctt
     1561 tcgttgagtc ttggattttt tacgtgtggc gaactctacc gcacatcttt ctttggaagc
     1621 ttgtttgttt aacgtggggc tcattcctac cgccacttct agagttactt atatcaaaaa
     1681 atatatgtga agtcaatttc gtagctgttc ttgactggac ttacttcgcc agcgtaatct
     1741 tctcctgaaa cgaaaaaacc accctggtgg gtggtttttc gaaggttatt taatcctggc
     1801 agattatcta accgtggtaa cagtcttgtg cgagacgtgt caccaaatct gtccttctag
     1861 tgtagcctca gttaggccac cacttcaaga actctaaata catctctcgc acatcctgtt
     1921 taccagtggc tgctgccaat ggcgataagt cgtgtctttc cggattggac tcaagacgat
     1981 agttaccggg acaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct
     2041 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat
     2101 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca
     2161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     2221 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaccgg caacggcgcg
     2281 gccttcccct tccatcgtcc ctcatcgctt tatcctttga tgtgacagcc ccctccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatctccggg
     2401 cttatctttg gcaccgtacg cgtcatagct gcatttttta atacgatgca ggatagggct
     2461 aacgccgcaa agtgacgtca cctgacgttc ttgggttagc aaggttaaag cagcggacag
     2521 gaaatgtttt gtgcccatag ccatgctggc ttattcataa aacagcggga caagatgtgt
     2581 ttttggagta ccgccggcac gccaatgact cctggtccgg ctccgcccga cacccgtatg
     2641 tttgctctct ttttgctgcc cttttgctat cgatttgcta cctgtttgct atctgtttgc
     2701 tatctgtttg ctatcgctac cctcagcagt gaaaagagta aggcacgtta gcgatgtata
     2761 catcgccgcg ccgcaagggt gccccttccg aatcccgttc atcactgcgt gctgattaat
     2821 ccaggttaaa tccgaccccc ttagcaataa atccttccat gcctgacggc ctggccaacg
     2881 cctcacggcc gcacagtaaa ctgcgcaccc gttcaccgtt atcagggtgg ttcatgatgg
     2941 ctttcagggc gctcgccgga ttttagccgc taaaatggcg gccatgcgtt cgtgtgcata
     3001 gccatgcaga cgcgaagaaa ttaactgtgg cttaaaagcg gtttttaacg aggtttaacc
     3061 ctgtctttca ggcaggaggg cgcagaaacg ctcccagacc gcctgaggcg gtgcgggcaa
     3121 cagatgttat ggtaaatgat aagggtatta cgttaagcac aaatcatctg caggtataaa
     3181 aattagtttc tataagcggc tttcttgcac ctacttggtg atttttctgg atgcgcttac
     3241 gttgctcacc tttaaatcac cataaaaaaa taacctttac ttacatatag atcgatctgg
     3301 ttttgtatcc tgacgagatt caatacgagc aagtatttaa agttttcttc cactgttttt
     3361 gcgttactcg caaaagccag tacaaaagat cttgaaaaca accgcgctcc ctggttacac
     3421 ttctgtataa gaactgatcg atgtctaaaa cggattgcat tatggctttt atctctctat
     3481 cgaacttggc agcgaagact ggaaggataa tccagtcgca gtatgttaca gccacgcaaa
     3541 cgaaaaaaag cacgggcgct gtcgagctgg gattccgctt gagcaaaagc gctatggacg
     3601 agactggaat taccattggg gaccgtgtcg acattcttta cgacaaagaa gaaaaccagt
     3661 ggatgattaa gaaaattagt gaaggtggtt ttggtgtgtc tgggc
//
LOCUS       DAMZII010000249         3665 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZII010000249 DAMZII010000000
VERSION     DAMZII010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.252"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(20..1369)
                     /gene="lysC"
                     /locus_tag="POA77_004764"
     CDS             complement(20..1369)
                     /gene="lysC"
                     /locus_tag="POA77_004764"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDI6368076.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            1894..3543
                     /gene="pgi"
                     /locus_tag="POA77_004765"
     CDS             1894..3543
                     /gene="pgi"
                     /locus_tag="POA77_004765"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6368077.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          924 a          975 c          935 g          831 t
ORIGIN      
        1 atacgccagt agggaaatat tactcaaaca aattaaaatg cagcttctgc accacctgct
       61 cggcatcttc gccgggcacc aggaagcaca ggttatggct ggatgcgccg taacaaatca
      121 tgcgaatgtt gaacggttcc agtacgccga atacctcttt gccaacgccg caggcttttg
      181 acaggtcatt gccaatcaac gcgaccagcg ccagaccttc ttccacctcc acccggcaca
      241 gtgcggaaag ctccatcagc agagattgcg tcagcaacgt atcgccagtg gaggttgaac
      301 cggtggtatc aagggttaat gccacgctca cttctgacgt ggtgattaag tctaccgaaa
      361 tattatgccg cgcgaggatg ccgaaaactt ccgcgaggaa gccgcgagaa tgcagcatat
      421 tcaggctgtg caaagtgagc agagtctgat tgcgacgaag cgccagcgcg cggaacagcg
      481 gcggattttc agttttattg cacaccagcg taccacctgc gcgtgggtct ttgctggagc
      541 cgacaaagac cgggatatcg ctgcgtactg cgggtagcaa cgttgccgga tgcagtactt
      601 ttgcaccaaa agttgccatc tctgccgctt cggcaaacgc gatttcatca atgcgttttg
      661 ctgcggaaac tacgcgtgga tcggtggtgt agatgcccgg gacgtcggtc cagatatcaa
      721 cacgagatgc gtgtaaagcc tccgccagca aggctgccgt ataatcgctg cctccacggc
      781 caagcgtcgt tgtacgacct ttattttcgc taccgataaa tccctgggtg atcactaagc
      841 cgtcattgag gcgtgggagc agctgcagcg cggccagttc cgccagcgcg gctacatctg
      901 gctctgcacg accaaatcgg tcgttggtac gcatcacttt acgtacatca aaccactgtg
      961 cctgaacatc gcgttcgcgc aggatttcga caaacagcag ggtcgacatc agctcgccgt
     1021 ggctgaccag ctcatctgtc agcgccggag acgttgccag cgccgccgct tctgccagaa
     1081 cagtaatgtt ctccagcaga cgttcaatct cttcacggat aacgttcggg taacgcagac
     1141 gttccagaat ggcaaactgg atgttgcgaa tagcgtcgag tttttcgaat cgctcgccag
     1201 gttccagccc ttccgctaaa gcgaccagca gattagtgat accagcagaa gccgagagga
     1261 ctactaaacg cacgttggca tcagaaagca caatatcagc gctgcggttc atggcgtcaa
     1321 aatcagctac gctggtaccg ccaaatttgg agacaacaat ttcagacata actacctcgt
     1381 gtcaggggat ccattttcag ccttggcaca agggaagagc ggaagacggg tgggcgcaga
     1441 gcgatacttc gctactattt tcacccagaa gtgctccacc acttgcgaaa cgcccgactg
     1501 cgaacgcttc tggtgacaac ccaggggatt cagcccctgt agccgatgat gaacgtggcc
     1561 agccgttcaa tcacctcggc gatgcacccc ctcaggtgtt atcacaggac tggctcctcc
     1621 aacaccgtta cttgggcaac gcgcctcttc tggcctgcgc tagcgcaggt agtacattta
     1681 taaataaagg gtgagcgggg cggttgtcaa cgacggggtc atgcggattt ttcatccact
     1741 cctggcggtc agtagttcag ctaataaatg cttcactgcg ctaagggttt acactcaaca
     1801 ttacgctaac ggcactaaaa ccatcacatt tttctgtgac tggcgctaca atcttccaaa
     1861 gtcacaattc tcaaaatcag aagagtattg ctaatgaaaa acatcaatcc aacgcagacc
     1921 gctgcctggc aggcactaca gaaacacttc gatgaaatga aagacgttac gatcgccgat
     1981 ctttttgcta aagacggcga tcgtttttct aagttctccg caaccttcgg cgatcagatg
     2041 ctggtggatt actccaaaaa ccgcatcact gaagagacgc tggcgaaatt acaagatctg
     2101 gcgaaagagt gcgatctggc gggcgcgatt aagtcgatgt tctctggcga gaagatcaac
     2161 cgcactgaaa accgcgctgt gctgcacgta gcgctgcgta accgtagcaa taccccgatt
     2221 ttggttgatg gcaaagacgt aatgccggaa gtcaacgcgg tgctggagaa gatgaaaacc
     2281 ttctcggaag cgattatctc cggtgagtgg aagggttata ccggcaaagc aatcactgac
     2341 gtagtgaaca tcgggatcgg cggttctgac ctcggcccgt acatggtgac cgaagcactg
     2401 cgtccgtaca aaaatcacct gaacatgcac tttgtttcta acgtcgatgg gactcacatc
     2461 gcggaagtgc tgaaaaaagt aaacccggaa accacgctgt tcctggtagc atctaaaacc
     2521 ttcaccactc aggaaaccat gaccaacgcc catagcgcgc gtgactggtt cctgaaagcg
     2581 gcaggtgatg aaaaacacgt tgcgaaacac tttgcggcgc tctcaaccaa tgccaaagcc
     2641 gttggcgagt ttggtattga tactgccaac atgttcgagt tctgggactg ggtcggcggc
     2701 cgttactctt tgtggtcagc gattggcctg tcgattgttc tctccatcgg ctttgataac
     2761 ttcgttgaac tgctttctgg cgcacacgcg atggacaagc atttctccac cacgcctgcc
     2821 gagaaaaacc tgcctgtact gctggcgctg attggcatct ggtacaacaa tttctttggt
     2881 gcggaaactg aggcgattct gccgtatgac cagtatatgc accgtttcgc ggcgtacttc
     2941 cagcagggca atatggagtc caacggtaag tatgttgacc gtaacggtaa cgttgtggat
     3001 taccagactg gcccgattat ctggggtgaa ccaggcacta acggtcagca cgcgttctac
     3061 cagctgatcc accagggaac caaaatggta ccgtgcgatt tcatcgctcc ggctatcacc
     3121 cataacccgc tctctgatca tcaccagaaa ctgctgtcta acttcttcgc ccagaccgaa
     3181 gcgctggcgt ttggtaaatc ccgcgaagtg gttgagcagg aatatcgtga tcagggtaaa
     3241 gatccggcaa cgcttgacta cgtggtgccg ttcaaagtgt tcgaaggtaa ccgcccgacc
     3301 aactccatcc tgctgcgtga aatcactccg ttcagcctgg gtgcgttgat tgcgctgtat
     3361 gagcacaaaa tctttactca gggcgtgatc ctgaacatct tcaccttcga ccagtggggc
     3421 gtggagctgg gtaaacaact ggcaaaccgt attctgccag agctgaaaga tgataaagaa
     3481 atcagcagcc acgatagctc gaccaatggt ctgattaacc gctataaagc gtggcgcggt
     3541 taatttaaaa accttaataa caatgccgac gttatgtcgg cattgtttta tcagataaat
     3601 ccccttgtct gtgatttaac ggaaatcata ccgtgaggtt aatcctaaaa tagattttta
     3661 atcgt
//
LOCUS       DAMZII010000250         3662 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZII010000250 DAMZII010000000
VERSION     DAMZII010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.253"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(46..90)
                     /locus_tag="POA77_004766"
     CDS             complement(46..90)
                     /locus_tag="POA77_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368078.1"
                     /translation="MKRNDKILHYEGLN"
     gene            209..1933
                     /gene="ilvI"
                     /locus_tag="POA77_004767"
     CDS             209..1933
                     /gene="ilvI"
                     /locus_tag="POA77_004767"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDI6368079.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMAFPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1936..2427
                     /gene="ilvN"
                     /locus_tag="POA77_004768"
     CDS             1936..2427
                     /gene="ilvN"
                     /locus_tag="POA77_004768"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI6368080.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2439..2525
                     /locus_tag="POA77_004769"
     CDS             2439..2525
                     /locus_tag="POA77_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDI6368081.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2607..3611
                     /gene="cra"
                     /locus_tag="POA77_004770"
     CDS             2607..3611
                     /gene="cra"
                     /locus_tag="POA77_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDI6368082.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          850 a          875 c         1019 g          918 t
ORIGIN      
        1 aaatagagaa attgctgtaa gttgtgggat tcagccgatt tattgtcaat ttaatccttc
       61 gtaatggagg attttatcgt ttcttttcac ctttcctcct gtttattctt attaccctgc
      121 gtttatgtct ctggctgcca attgcttaag caagatcgga cggttaatgt gttttacaca
      181 ttttttccgt caaacagtga ggcaggccat ggagatgttg tctggagccg agatggtcgt
      241 ccgatcgctt atcgatcagg gcgttaaaca agtattcggt tatcccggag gcgcagtcct
      301 tgatatttac gatgcattgc ataccgtggg tggtattgat catgtattag ttcgtcatga
      361 gcaggcggcg gtgcatatgg ccgatggcct ggcgcgtgcg accggggaag tcggcgtcgt
      421 gctggtaacg tcgggtccag gggcgaccaa tgcgattacc ggcatcgcca ccgcttatat
      481 ggattccatt ccattagttg tcctttccgg gcaggtagcg acctcgttga taggttacga
      541 tgcctttcag gagtgcgaca tggtggggat ttcgcgaccg gtggttaaac acagttttct
      601 ggttaagcaa acggaagaca ttccgcaggt gctgaaaaag gctttctggc tggcggcaag
      661 tggtcgtcct ggaccagtag tcgttgattt accgaaagat attcttaatc cggcgaacaa
      721 attaccctat gtctggccgg agtcggttag tatgcgttct tacaatccca ctactaccgg
      781 acataaaggg caaattaagc gtgctctgca aacgctggtg gcggcaaaaa aaccggttgt
      841 ctacgtaggc ggtggggcaa tcacggcggg ctgccatcag cagttgaaag aaacggtgga
      901 ggcgttgaat ctgcccgttg tttcctcatt gatgggcctg ggggcatttc cggcaacgca
      961 tcgtcaggcg ctgggcatgc tgggaatgca cggcacctac gaagccaata tgacgatgca
     1021 taacgcggat gtgattttcg ccgtcggggt aagatttgat gaccgaacga cgaacaatct
     1081 ggcaaagtac tgcccaaatg ccactgttct gcatatcgat attgatccta cttccatttc
     1141 taaaaccgtg actgctgata tcccgattgt gggggatgct cgccaggtcc tcgaacaaat
     1201 gcttgaactc ctgtcgcaag aatccgccca tcaaccgctg gatgagatcc gcgactggtg
     1261 gcagcagatt gaacagtggc gcgctcgtca gtgcctgaaa tatgacactc acagtgaaaa
     1321 gattaaaccg caggcggtga tcgagactct ttggcggttg acgaagggag acgcttacgt
     1381 gacgtccgat gtcgggcagc accagatgtt tgctgcactt tattatccat tcgacaaacc
     1441 gcgtcgctgg atcaattccg gtggcctcgg cacgatgggt tttggtttac ctgcggcact
     1501 gggcgtcaaa atggcgtttc cagaagaaac cgtggtttgc gtcactggcg acggcagtat
     1561 tcagatgaac atccaggaac tgtctaccgc gttgcaatac gagttgccgg tactggtggt
     1621 gaatctcaat aaccgctatc tggggatggt gaaacagtgg caggacatga tctattccgg
     1681 ccgtcattca caatcttata tgcaatcact acccgatttc gtccgtctgg cggaagccta
     1741 tggtcacgtc gggatccaga tctctcatcc gcatgagctg gaaagcaaac ttagcgaggc
     1801 gctggaacag gtgcgcaata atcgcctggt gtttgttgat gttaccgtcg atggcagtga
     1861 gcacgtctac ccgatgcaga ttcgcggggg cggaatggat gaaatgtggt taagcaaaac
     1921 ggagagaacc tgattatgcg ccggatatta tcagtcttac tcgaaaatga atcaggcgcg
     1981 ttatcccgcg tgattggcct tttttcccag cgtggctaca acattgaaag cctgaccgtt
     2041 gcgccaaccg acgatccgac attatcgcgt atgaccatcc agaccgtggg cgatgaaaaa
     2101 gtgcttgagc agatcgaaaa gcaattacac aagctggtcg atgtcttgcg cgtgagtgag
     2161 ttggggcagg gcgcgcatgt tgagcgggaa atcatgctgg tgaaaattca ggccagcggt
     2221 tacgggcgtg acgaagtgaa acgtaatacg gaaatattcc gtgggcaaat tatcgatgtc
     2281 accccctcgc tttataccgt tcaattagca ggcaccagcg ataagcttga tgcattttta
     2341 gcatcgattc gcgatgtggc gaaaattgtg gaagttgctc gctctggtgt ggtcggactt
     2401 tcgcgcggcg ataaaataat gcgttgagaa tgatctcagt gcgcaatttg cagcccaaca
     2461 tgtcacgttg ggcttttttt gcgaaatcag tgggaacctg gaataaaagc agttgccgca
     2521 gttaattttc tgcgcttaga tgttaatgaa tttaacccat accagtacaa tggctatggt
     2581 ttttacattt tacgcaaggg gcaattgtga aactggatga aatcgctcgg ctggcgggag
     2641 tgtcgcggac cactgcaagc tatgttatta acggcaaagc gaagcaatac cgtgtgagcg
     2701 acaaaaccgt tgaaaaagtc atggctgtgg tgcgtgagca caattaccac ccgaacgccg
     2761 tggcagctgg gcttcgtgct ggacgcacac gttctattgg tcttgtgatc cccgatctgg
     2821 agaacaccag ctatacccgc atcgctaact atcttgaacg ccaggcgcgg caacggggtt
     2881 atcaactgct aattgcctgc tcagaagatc agccagacaa cgaaatgcgc tgtattgagc
     2941 accttttaca gcgtcaggtc gatgccatta ttgtttcgac gtcgttgcct cctgagcatc
     3001 ctttttatca acgctgggct aacgacccgt tcccgattgt cgcgctggac cgcgccctcg
     3061 atcgtgaaca cttcaccagc gtggttggtg ccgatcagga tgatgccgaa atgctggcgg
     3121 aagagttacg taagtttccc gccgagacgg tgctttatct tggcgcgctg ccggagcttt
     3181 ctgtcagctt cctgcgtgaa caaggtttcc gtactgcctg gaaagatgat ccgcgcgaag
     3241 tgcatttcct gtatgccaac agctatgagc gggaggcggc tgcccagtta ttcgaaaaat
     3301 ggctggaaac gcatccgatg ccgcaggcgc tgttcacaac gtcgtttgcg ttgttgcaag
     3361 gggtgatgga tgtcacgcta cgtcgcgatg gtaaactgcc ttctgacctg gcaattgcca
     3421 cctttggcga taacgaactg ctcgacttct tacagtgccc ggtgctggca gtggctcaac
     3481 gtcaccgcga tgtcgcagag cgtgtgctgg agattgtcct ggcaagcctg gacgaaccgc
     3541 gtaagccaaa acctggttta acgcgcatta aacgtaatct ctatcgccgc ggtgtgctca
     3601 gccgtagcta agccgcgaac aaaaatacgc gccaggtgaa tttccctctg gcgcgtagag
     3661 ta
//
LOCUS       DAMZII010000251         3636 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZII010000251 DAMZII010000000
VERSION     DAMZII010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.254"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(183..440)
                     /locus_tag="POA77_004771"
     CDS             complement(183..440)
                     /locus_tag="POA77_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368083.1"
                     /translation="MKNEFILMKLVARGLLDIRIAADSGNVKACHMLSDFIHTLPYAI
                     ERVLKGEIDYQYVMDNLNERAKIKNMEGWLANALRDINASE"
     gene            complement(442..>3636)
                     /locus_tag="POA77_004772"
     CDS             complement(442..>3636)
                     /locus_tag="POA77_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6368084.1"
                     /translation="TRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTV
                     RYGYNSQLQLTSVTYPDGLRSSRKYDRQGRLAEEISRNGNITRWFYDSSRSGLPCAVE
                     DGTGVRRRITRNRYGQLQAFTDCSGYATRYEYDRYGQQIAIHREEGISTYSSYNPRGQ
                     LVSQKDAQGRETRYEYSAAGDLTATISPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYD
                     AAGRITTLTNENGSQSTFLYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITL
                     WHYDASDRITHRTVNGDPAEQWQYDEHGWLTTISHTSEGHRVAVHYGYDDKGRLTGER
                     QTVENPETGEILWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPL
                     VEYTRDRLHRETVRSFGGEACELATAWNTSGQLQSRHLNLPQLDCDYTWNDNGQLIRI
                     SGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPD
                     NRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHG
                     EPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTT
                     RIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAVMLGRLERE
                     LRAGAVSAESEAWLAQCGLTAEQMAAQMEAEYIPERKLHLYHCDHRGLPQALISPEGE
                     TAWCGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQD
                     PIGLKGGWNLYAYPLTPVQRGDPLGLSGETLVLGSATTVTGTKFGGALLSGGAATFDA
                     VTTAMASAAPALGAMAVVAGPMGYDLLANDGRAGKALSDGQLINSQAEMCYWTSSCPL
                     DSPHENDNSSGSQPNVGKDLTDEEKNCLAGGSAGSPGGWGPEDEEKARSRGRGSDTEA
                     KIKDLDLLHSPETIGSRPDLAKLTDSELLESVINPKSGDRVTVNTKTNKVMDGNSRVY
                     ELKKRADDPNSSITGETTIPVETYTPDDSFFWDM"
BASE COUNT          701 a         1168 c          850 g          917 t
ORIGIN      
        1 tacaagtata tccttatttt aaagttaata gtatttcaca tcaagaaaaa taccatatca
       61 taatattttt aaagattttt ttataatctt gtcctgctgc actcacagaa cgtggttttt
      121 cagttagcaa taaaaaccgg gagaattaat atacttcccg gcttattatt tatcaaagga
      181 tattactctg acgcatttat atctctcaac gcattagcaa gccaaccttc catgtttttt
      241 atcttggccc tttcatttaa attatccatc acgtactgat aatcaatttc accttttaaa
      301 accctctcaa tagcatacgg taaagtatgt ataaaatcag ataacatatg acaagctttc
      361 acattaccag aatctgctgc tattcttata tccagcaatc ctctggcgac taacttcatg
      421 agaataaatt catttttcat attacatgtc ccaaaaaaaa gaatcatcag gtgtgtaagt
      481 ttctactggt atcgtagttt caccggttat tgagctatta ggatcatcag ctcttttttt
      541 caactcgtat acacgagaat taccatccat tactttattt gtttttgtat taacagttac
      601 tcgatcacca gacttaggat taataacaga ctctaacaac tcactgtctg ttagtttagc
      661 aagatcagga cgcgagccaa tagtttccgg cgaatgaaga agatccagat cctttatttt
      721 tgcttctgta tcagaaccac gcccccgact gcgagccttt tcctcatcct ctggacccca
      781 tcctcccgga cttcctgcac tgccgccggc caggcagttc ttctcttcat ccgtcaaatc
      841 cttcccaaca ttcggctgac tccctgacga attatcattc tcatgtgggc tatctaaagg
      901 acaactactg gtccagtaac acatttcagc ctgagaattt attaactgcc catcgctcag
      961 agccttcccg gcacgcccat cattagcgag aaggtcatat cccattggcc cagcgaccac
     1021 agccattgct ccaagagcag gggcagcaga cgccatagcc gtcgtgactg catcaaaagt
     1081 cgccgcccca cccgaaagta acgccccccc aaattttgtt cctgttactg ttgtggcaga
     1141 accaagaacc agtgtctcac cagacaaccc aagaggatcc ccccgctgca ccggggtcag
     1201 cggatacgca tacaggttcc atcccccctt cagcccaatg ggatcctggg tgatatagcg
     1261 tccctgcaac ggatcgtagt accggttccg gttatagtac agccccgact cctcatcata
     1321 ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc gcactggttt cgcccagcag
     1381 gtttccccat tcgtcatact ccccgcacca cgccgtttcc ccttccgggc tgataagcgc
     1441 ctgcggcagt ccccggtggt cacagtggta aagatgaagt ttcctctccg ggatgtattc
     1501 cgcctccatc tgcgcggcca tctgttccgc cgtcagcccg cactgcgcaa gccatgcctc
     1561 actctccgca ctcaccgctc ccgcgcgcag ttcccgctcc agcctcccca gcatcaccgc
     1621 cagctccgcc ggtagcgtca cacccgtgtc ctcctgcaac acctccgcca ggctccggtg
     1681 ccgcgccttc gcctgttcgc cattctctgt ttcgattctc aggagcggcg tgaagctccc
     1741 cggctggtac accgtctgga tacgcgtggt gccggtctgt atcgtcgtca gcctgtcacc
     1801 gtcccacccg taccatgtca cctccggttt acgcgacagc gacatccacc ccgtcaggtc
     1861 acgctcccgc cgccataccc gtttcgccat tcgccgtccc agcgggtcgt agaggtagcg
     1921 gctctcgacc agtggctcgc catgctgtat ccgcgtgtag aacaccaggc ggtgctggct
     1981 gtcgtagtgg tagtggtggg tccgctcgtc gtgcatccgt atcacccctt ccgggatgcg
     2041 gtccgtcttc tccgccagcc tgccgtattc atcgtggcgg tagacatagt gcgcatcttc
     2101 cgcgatgcgg ttatccggcc atgccgtgag ggtgctgtcc gggtgcagtt ccgggtccgg
     2161 cagccggttt cctgccgggt ccgtcgcata cgggatatcg atatccaggt tcgccgccgt
     2221 ggtgtggacg cccgtcagcc ttcccgtgtc gctgtaacgg tactcccggc tctcctgcgg
     2281 gccgctgatg cggattagtt gtccgttatc gttccacgta tagtcacagt caagctgcgg
     2341 aaggttcagg tgcctgctct gtagctggcc gctggtattc caggcggtgg caagttcaca
     2401 ggcctccccg ccgaagctgc gcaccgtctc ccggtgcagc cggtcccgcg tgtactcgac
     2461 cagtggcgtt ccgcccagct tcatccccgc aagataaccg ctgccatacg tcagccactc
     2521 caccggcggc aggccgtccg gctcctgacg ggtcgccagc ccctgttcgc tgtatgcatg
     2581 ccccgtctca tgctcccaca gtatctcccc cgtctccggg ttctccaccg tctgccgttc
     2641 gcccgtcagg cggcctttat cgtcatagcc gtagtggacc gccacgcggt ggccttcact
     2701 ggtatggctg atggtggtta accacccgtg ctcatcatac tgccactgct ccgccgggtc
     2761 gccgttcacc gtccggtgcg tgatgcggtc cgacgcatcg tagtgccaga gggtgataag
     2821 tccctcgtct tcactctgcg tgagttttcc ggtcaggtca tactggtaac gttgcgtcct
     2881 gccatcaaag ccgcgctgct ccgtcagcct gtccaccggg tcatacagga acgtggactg
     2941 gctgccgttc tcattagtca gcgtggtgat gcgcccggcg gcgtcatacc ccatgctgcg
     3001 cgtcagtccg ccttccgtca cactcaccgg acgcccgcgt ttatcatatt cgatggtgct
     3061 gcgtttaccg tccggtgata tcgtcgctgt caggtcgcct gcggcgctgt actcataacg
     3121 ggtttcacgc ccctgcgcat ccttctgact gaccagttgg ccacgcggat tataactgct
     3181 gtaagtgctg atgccttctt cccggtgaat ggcgatttgt tgaccgtacc ggtcatattc
     3241 ataccgcgtc gcgtaccccg agcagtccgt aaaggcctga agctgaccgt accggtttcg
     3301 cgttattctc cgcctcaccc cggttccgtc ttccaccgca cagggcaggc cactgcggga
     3361 agaatcataa aaccagcgcg taatgttacc gttgcgcgag atctcttctg ccagtcggcc
     3421 ctgacggtca tatttccggc tgctgcgtaa accatccgga tatgtcactg aagtcagttg
     3481 caactggctg ttatagccat accgcaccgt cctgccgtcc ggcagtatca ctgaagtcag
     3541 ctttccagac gccatatgca gccggtactc cgtccgccgc cccgccgcat ccgtctgtgc
     3601 cttcagcctc cccgcctcat catactcgct gcgggt
//
LOCUS       DAMZII010000252         3536 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZII010000252 DAMZII010000000
VERSION     DAMZII010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.255"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1098
                     /locus_tag="POA77_004773"
     CDS             <1..1098
                     /locus_tag="POA77_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDI6368085.1"
                     /translation="TVERHIRGKWACDSCETLIQAPVPPQVIDKGIPTAGLLAQVLVA
                     KYADHLPLYRQERMFGRAGLEIPRSTLAEWVGACGVQLQPLVDALRNTLLEHSVLHAD
                     ETPVSMLAPGKKKTHKAYVWAYCTTPFADLKAAIYDFAPSRAGEHARTFLGDWRGKLV
                     CDDYAGYKAGFGNGITEIGCMAHARRKFYDLHEANKSELAAKALEYIGGLYEIERETK
                     DLPPDMRREIRQTKAKPLADALHQWMLAHRQKVPDGSGTAKALDYSLKRWEALTRYLD
                     DGAVPIDNNWVENQIRPWALGRSNWLFAGSLRSGQRAAAVMTLIQSAKLNRHDPYAYL
                     KDVLTRLPTQKNNAIDELLPHNWKPAIPNKV"
     gene            complement(1349..1912)
                     /locus_tag="POA77_004774"
     CDS             complement(1349..1912)
                     /locus_tag="POA77_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HDI6368086.1"
                     /translation="MNTRLFTFAGGETGVWRVVRMDAVAGAPLPGIPRLDVAAGSVSP
                     QPLGTKWLLRGITSNERYVVREEKDRLVAKQPSLGRAEATCAALIPIRKNPSWWGLAQ
                     DERRKIFEEQSRHIHIGLQYLPAVARRLHHCRDLGESEPFDFLTWFEYSPSDEPGFNR
                     LLAELRASVEWKYVDREIDIRLVHEPA"
     gene            complement(1936..2310)
                     /locus_tag="POA77_004775"
     CDS             complement(1936..2310)
                     /locus_tag="POA77_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI6368087.1"
                     /translation="MGVNMRAETESRIFSVDEYVRPSNGEPIRSVVLETNDSVVVVWH
                     AHPGQEIASHVHPHGQDTWTVISGEAEYHQGNGIVTHLKAGDIAIAKPGQVHGAMNSG
                     PEPFIFVSVVAPGNAGFALAEK"
     gene            complement(2375..2938)
                     /locus_tag="POA77_004776"
     CDS             complement(2375..2938)
                     /locus_tag="POA77_004776"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6368088.1"
                     /translation="MAHQIFRSSCSITPLAVPRRCRSASWRQKGVERIGCWACAAQVH
                     QKTQSKGKIGRAEMALTLINKLYGIERDMEEAGDAQRYQTRQSQSQAFLEQLKARLDK
                     TPPQVTSSKCLGQAINCLASNWLKLIRYIERGTPAHRQQPCRAGEPPSHVHMTKASPV
                     SSRYVMPTHDGVLTVSVRQSIVFPKGA"
     gene            3181..3516
                     /locus_tag="POA77_004777"
     CDS             3181..3516
                     /locus_tag="POA77_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6368089.1"
                     /translation="MAQGGITGRLRYNGQRLRLLSIDTPETYKPRCDAELTKGREATA
                     RLRELVANAQRIEVVDSGQADKYDRRLVHLLIDGRDVGQTLMAEGLAVEWRPGPNAWR
                     ERRRHWCGY"
BASE COUNT          790 a         1007 c         1021 g          718 t
ORIGIN      
        1 accgttgaac gtcacatccg gggcaaatgg gcctgcgaca gctgcgagac gttgatccag
       61 gcaccggtgc caccgcaggt gatcgacaaa ggcatcccca ccgctggcct gctggcccag
      121 gtgctggtgg ccaagtatgc cgaccatctg ccgctgtacc gtcaggaacg catgttcggc
      181 cgtgccggcc tggaaatccc gcgctccacc ctggccgaat gggtgggtgc ctgtggtgtg
      241 cagttgcagc cgctggtgga tgcgttgaga aatactctgc tggaacacag tgttctgcat
      301 gccgatgaaa ccccggtcag catgctggcc ccgggcaaga agaaaaccca caaggcctac
      361 gtctgggcct actgcaccac accgtttgcc gacctgaagg cagctattta cgacttcgca
      421 cccagccgtg caggcgaaca cgcccgaacc ttcctgggtg actggcgggg caagctggtc
      481 tgtgatgatt acgctggcta caaggccggg ttcggcaacg gcattaccga aatcggctgt
      541 atggcccacg cccggcgcaa gttctatgac ctgcacgagg ccaacaagag cgaattggcg
      601 gccaaggcct tggaatacat cggtggactc tacgagatcg agcgggaaac caaagatctg
      661 ccaccggata tgcggcggga aatccggcaa accaaagcca aaccccttgc cgatgcgctg
      721 catcagtgga tgctggccca tcgccagaaa gtgccggatg gctccggcac ggccaaagcc
      781 ctggattaca gcctcaaacg ttgggaagcg ctgacgcgct acctggacga tggcgctgtg
      841 cccatcgaca acaactgggt ggaaaaccag atccgaccct gggcgctggg gcgcagcaat
      901 tggctgtttg cgggttcact gcgcagtggt caacgtgcgg ctgcggtcat gaccctaata
      961 cagtcagcga agctgaatcg gcatgatccc tatgcctacc tgaaggatgt gctgactcgc
     1021 ctgccgacac agaagaacaa cgccatcgac gagctattgc cccacaactg gaaaccggcc
     1081 atacctaaca aggtgtaggc cggacgtgta tggagagcct tcttcgaaag ctggacaagg
     1141 tgccaactct agacggcagg atatgccctg ggagatcgat gagagaaaga ggaggccata
     1201 agtagaaaat ttcccaagcg actggtggct cgctaggcat acgtgtcgtg ttactcctaa
     1261 gttattagac acactagatt ttccgggctt tgctgggctg cgggtgtcgc aggtgtatgc
     1321 cgcacggaga tcgcagttaa acctgatact aggccggctc atgcactagc cggatgtcga
     1381 tttctctgtc gacatacttc cactccaccg aggcccggag ttcggccaaa agcctgttaa
     1441 agcccggctc atcagacggg gaatattcga accacgtcaa gaagtcgaag ggttcgctct
     1501 cgcccaggtc ccgacaatgg tggagacgac gggccacagc agggagatat tggagcccaa
     1561 tgtgaatgtg acgggactgc tcctcaaaaa tcttccggcg ttcgtcttga gccagtcccc
     1621 accatgaggg gtttttccgg atagggatca gggcggcaca ggtggcctct gctcgaccca
     1681 aacttggctg cttcgccacg agacgatcct tctcttcacg cacgacataa cgctcgttac
     1741 tcgttatccc gcgaagaagc catttggtgc ccaaaggctg cggcgaaaca gagccagcgg
     1801 ctacgtcgag ccgggggatt ccagggagag gggctcccgc cacggcatcc atccgaacaa
     1861 cgcgccaaac acccgtttct ccaccagcga acgtaaataa tcgtgtattc atcagtttcc
     1921 tctcaggcct ttcgcctatt tctcagccaa cgcaaaaccg gcattgcccg gcgcaaccac
     1981 tgaaacaaat ataaaaggtt cggggccaga gttcattgca ccatgtacct gcccaggttt
     2041 tgcgatcgca atatctccag ctttaagatg agtgactatg ccgttacctt gatgatattc
     2101 ggcttcacct gagataaccg tccaagtatc ttggccgtga ggatgtacat gggaagctat
     2161 ttcctgccca gggtgggcgt gccaaacaac gacgacagag tcattggttt ccagaacaac
     2221 cgaacgaatc ggttcgccgt tagacggacg aacatattca tccaccgaaa aaatccgcga
     2281 ttcagtttct gctctcatgt tcacacccat ttaaaactga ttggacacgt actctaaacg
     2341 caactgcgcc tggacagcac atgaagatat gtacctaagc gcccttcggg aaaactatgc
     2401 tctggcgcac agagaccgtc aatactccgt cgtgggtagg catgacataa cgggaggaga
     2461 cgggcgacgc tttggtcata tgtacatgag aaggtggctc gcccgctcgg cagggttgtt
     2521 gtcgatgggc aggtgtcccc ctttcgatgt agcggatcag ctttaaccag ttgctcgcaa
     2581 ggcaattgat tgcttgccca aggcactttg acgacgtgac ctggggaggg gttttgtcca
     2641 gcctagcttt cagttgctcc aggaaagcct ggctttgcga ctggcgcgtc tggtatcgct
     2701 gggcatcgcc cgcctcttcc atgtcgcgct cgatgccgta gagcttgttg ataagagtca
     2761 gtgccatctc cgctcggccg atcttgccct tggattgagt tttctgatga acttgcgccg
     2821 cgcatgccca gcaacctata cgctcgacgc ctttctgcct ccaggaggcg ctgcggcacc
     2881 tcctgggtac ggctagcggt gtaattgaac aggatgaccg gaagatttgg tgggccaccg
     2941 cgatctcctc agtagctata gccaaacagg ttgggtatga gaagttcaag ggggggcgag
     3001 caaagccccc gttccaattg attctctatt ccacacaaat ggcgatataa gccggcgggt
     3061 gatgacgacc ctggaagctc tggcatcgag agtggatgtc tactccatcg acgaggcgtt
     3121 tctggatctg accggaatcg accgggtggt aagcttcgag ccttttggtc ggcaggcgat
     3181 ttggctcaag gtggaatcac cggacggtta cgatacaacg gtcaacgctt gcgcctgctg
     3241 tcgattgaca ccccggaaac ctacaagccc aggtgcgacg ccgagctgac gaagggccgc
     3301 gaggcaaccg cacgcctccg cgaactggtt gccaacgctc agcgcatcga ggttgtcgat
     3361 tccggccaag ccgacaagta cgaccgccgc cttgttcacc tgctgatcga cgggcgggac
     3421 gtggggcaaa ccctcatggc cgaggggtta gccgttgagt ggaggcccgg cccgaatgcg
     3481 tggagggaac gccgtcgcca ctggtgcgga tactgatcgc ttttcagctt gagcca
//
LOCUS       DAMZII010000253         3515 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZII010000253 DAMZII010000000
VERSION     DAMZII010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.256"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            155..270
                     /gene="rrf"
                     /locus_tag="POA77_004778"
     rRNA            155..270
                     /gene="rrf"
                     /locus_tag="POA77_004778"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(374..901)
                     /gene="mobB"
                     /locus_tag="POA77_004779"
     CDS             complement(374..901)
                     /gene="mobB"
                     /locus_tag="POA77_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI6368090.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(883..1467)
                     /gene="mobA"
                     /locus_tag="POA77_004780"
     CDS             complement(883..1467)
                     /gene="mobA"
                     /locus_tag="POA77_004780"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI6368091.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1537..1806
                     /locus_tag="POA77_004781"
     CDS             1537..1806
                     /locus_tag="POA77_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDI6368092.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1883..2869
                     /gene="srkA"
                     /locus_tag="POA77_004782"
     CDS             1883..2869
                     /gene="srkA"
                     /locus_tag="POA77_004782"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDI6368093.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRYVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2886..3512
                     /gene="dsbA"
                     /locus_tag="POA77_004783"
     CDS             2886..3512
                     /gene="dsbA"
                     /locus_tag="POA77_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDI6368094.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          892 a          843 c          873 g          907 t
ORIGIN      
        1 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt
       61 acaacgccga aggtgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      121 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggccttagcg cggtggtccc
      181 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      241 tccccatgcg agagtaggga actgccaggc atcaaattaa gcagaaggcc atccgtcagg
      301 atggcctttt tgcattggcg cagaaaaaaa tgccggatgc gacgctggcg cgtcttatcc
      361 agcctactct tgcttatccg ttctgctttt gcatccactc caccacaaaa tcagccagcc
      421 cctcaacatc attaatatcc agtaatgcga catcaagatt aagcggcaca tcactggcta
      481 cagcaataac atgcctgtct atcactaatt cttcaggtcg atgtccggct ccatcgcgaa
      541 acagcacaat ctttgcgatc tcttcatgct taaacccttc gaccagaatc aaatccagct
      601 ttgaggtatc cattcgactt gcgagaaact gtagatccag ctcttcttcg tctggtgttt
      661 ccgtcatcaa ggcccatcgt tgctggctgg caacgatggt ttgtgccgcg ccagccttgc
      721 gcagctcata gctatctttg cctggcttat caacatccat atcatggtgc gtatgcttaa
      781 tcagccctgg acggatccct ctggcgcata atgccgggat cagttgtttc aaaagcgtag
      841 tttttccggt accactccac gcggcaaagg cgagtaacgg tatcatcgtt tttcctgcca
      901 tcgggcaagc tcctctggcg tattcacgtt aataaatgca tctttattat cgctgaaatc
      961 aaccgcatga ccgccagcca gacgcataaa tgccattacc cggcgttctc ctgcttgcag
     1021 atattccagt aataaaggct caattgcgcg gtttaccaga gcaatagtcg ggtgatcgcg
     1081 ttcaccgtcg tggacccaca cgacaggcgc atctttgcgc tgatgattaa gccgggctgc
     1141 taaatcctcg ggaatataag gagtatcgca cggacaaaac aaaaaccact cgcctgcttc
     1201 ctgctgcatt actgaaagca ttcctgccag agggcctggg taatccgcca gtgaatcttc
     1261 aatcactttc agaccgcttg cctgatagat ttcctgatgg cgattggcat taaccacgac
     1321 gtgagagagc tgcgtcataa gcgcgtcagc gacatgttgc cataatggtt tgccgtttaa
     1381 ttcaagcaat cctttatcta cgccgcccat tcgtctggct ttaccgcctg ccagcacaac
     1441 gcctgttatc gtcgtcatca gattcaccga tatcgcctct tttattgtgg gattgaccct
     1501 gctaacgtgt ctgtctcaag agtaaggagc attactatga aatgtaaacg tctgaatgaa
     1561 gttattgaac tcctccagcc agcctggcaa aaagagccag accttaacct gttgcaattt
     1621 ttgcagaaac tggcgaaaga gtcaggtttt gacggcgaac tggcggattt gacggatgac
     1681 attctgatct atcacctgaa aatgcgtgat tccgcgaaag atgcggtgat cccgggtttg
     1741 cagaaagatt atgaagaaga cttcaaaacg gcgctgttac gcgctcgcgg cgtaattaaa
     1801 gagtaaaagc ttgtaagcgg tgccaccaaa atcatcgtga aatgatatcc ttcatcattc
     1861 gtaatgtttt ccggatgatg ggatgaacaa cagcgctttt actttccaga cactacaccc
     1921 ggataccatc atggatgctc tatttgagca agggatccgg gtggattccg gtcttacccc
     1981 gcttaacagc tatgaaaacc gtgtctatca atttcaggac gaagatcgtc gacgttatgt
     2041 cgtcaaattt tatcgccctg aacgttggac agccgatcaa atcctcgaag aacatcaatt
     2101 tgcgttgcag ctggtaaatg atgaagttcc ggtcgcagca cctgtggcct ttaacggtca
     2161 gactttattg aatcatcagg gattttattt cgctgttttt ccaagcgtcg gtggtcgcca
     2221 gttcgaagct gataatatcg atcagatgga agcggttggg cgttatttag ggcgtatgca
     2281 ccagacgggg cgcaaacagc tttttatcca tcgcccgacc atcggtctga atgaatatct
     2341 cattgagcct cgcaagctgt ttgaggacgc tacactgata ccttccgggt tgaaagcggc
     2401 attcctgaaa gcgacagatg agctgatcgc cgccgttaca gcacactggc gggaagactt
     2461 caccgttctg cggctacatg gagactgcca cgccgggaat attctctggc gcgatggtcc
     2521 aatgtttgtt gatctggatg atgcacgtaa tggtccagct attcaggatt tgtggatgtt
     2581 gctcaatggc gataaagccg agcagtggat gcaactggaa actattattg aagcttatga
     2641 agaatttagc gagttcgaca ccgctgaaat cggactgatt gaacctttac gcgccatgcg
     2701 tttggtttat tatcttgcct ggctaatgcg gcgttgggct gatcccgcgt tcccgaaaaa
     2761 ttttccgtgg ttaaccgggg aagattactg gctgcgacag acggcgactt ttatagaaca
     2821 ggcaaaagtt ctacaagaac cccctttgca attaacacct atgtattaat cggagagagt
     2881 agatcatgaa aaagatttgg ctggcgctgg ctggtttagt tttagcgttt agcgcatcgg
     2941 cggcgcagta tgaagatggt aaacagtaca ctaccctgga aaaaccggta gctggcgcgc
     3001 cgcaagtgct ggagtttttc tctttcttct gcccgcactg ctatcagttt gaagaagttc
     3061 tgcatatttc tgataatgtg aagaaaaaac tgccggaagg cgtgaagatg actaaatacc
     3121 acgtcaactt catgggtggt gacctgggca aagatctgac tcaggcatgg gctgtggcga
     3181 tggcgctggg cgtggaagac aaagtgactg ttccgctgtt tgaaggcgta cagaaaaccc
     3241 agaccattcg ttcagcatct gatatccgcg atgtatttat caacgcaggt attaaaggtg
     3301 aagagtacga cgcggcgtgg aacagcttcg tggtgaaatc tctggtcgct cagcaggaaa
     3361 aagctgcagc tgacgtgcaa ttgcgtggcg ttccggcgat gtttgttaac ggtaaatatc
     3421 agctgaatcc gcagggtatg gataccagca atatggatgt ttttgttcag cagtatgctg
     3481 atacagtgaa atatctgtcc gagaaaaaat aattc
//
LOCUS       DAMZII010000254         3391 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMZII010000254 DAMZII010000000
VERSION     DAMZII010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.257"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            368..565
                     /locus_tag="POA77_004784"
     CDS             368..565
                     /locus_tag="POA77_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HDI6368095.1"
                     /translation="MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPH
                     DMVESSAAAWRDEMSRKVAGK"
     gene            562..744
                     /locus_tag="POA77_004785"
     CDS             562..744
                     /locus_tag="POA77_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6368096.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVQVHDNIITV"
     gene            843..1151
                     /locus_tag="POA77_004786"
     CDS             843..1151
                     /locus_tag="POA77_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368097.1"
                     /translation="MDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGL
                     WGISWGCAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWNDV
                     "
     gene            1711..2745
                     /locus_tag="POA77_004787"
     CDS             1711..2745
                     /locus_tag="POA77_004787"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDI6368098.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNLHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
BASE COUNT          950 a          706 c          813 g          922 t
ORIGIN      
        1 attatcatgt taaagtgtca tatcgtttac ataaagtcaa gatatatcag tgtggcaagc
       61 ctggcaggat tctctaggga agggcaggca tactcagtgc atagctgatg cacggatgac
      121 ggcagggggg aggaggctgg tgactttcgg acgactgtag cggcttacat cgggcttcgg
      181 cggtatcata tgaaacagcg gagtgccgcc ttccatgccg ctggcgcggc atatcctagt
      241 gacgatatct gaatcgttat atactgtgta tatacgtggt aatgacgggg tgataaatgg
      301 cacaggttaa tatgagttta agaattgacg ctgaactgaa ggatgctttt atggccctgc
      361 aaaaagcatg gaccgtaatg gctctcagtt aatccgggat tttatgcgcc agaccgtgga
      421 acggcagcat aatacctggt tccgtgacca ggttgaggca ggacgtcagc aactcgagcg
      481 cggcgatgtg cttccccatg acatggtcga atcttccgct gctgcatggc gggatgaaat
      541 gagcaggaaa gtcgccggta aatgatggag atattctgga ccatgctggc cagccaggac
      601 aggaagcgca ttcgtgagta cattgcggag cagaacctga tagccgccat tgaactggac
      661 gaacggattg gttgttcagc cagcctcctg ttctcccttt cttttatcag tgtacaggtg
      721 catgacaaca taatcacggt atgataataa taatcaataa caataagttg tgtcacgttt
      781 acatcatgga acggaggtgc tcctgtgact tttgagcaaa aaaagcccgg gcaattgccc
      841 tgatggacag caaaaagatg tggcggagta attatgctcc tcctctgctg cgtattctct
      901 ggcggctggg tatccggctt ccgccattgc cctttatgcc gttctggcag gttacgctgc
      961 tgatgggggg cttatggggt atttcctggg gatgtgcaat gtggtttatg tactgggggc
     1021 cgtcaggaat ggtagcaggt gaggccatta ttataagtat cacgagtggt tttttgttcg
     1081 gactgctcat ggcctctttc cactggtggc gcaggaaagt taaccggctg ccaccatgga
     1141 atgatgtgta aaccgtgccg gcatgttttt acggggtgat gctttcatta acctgtacac
     1201 gaacttctat tcctgcatca ttacaggcct acagccacta cgccacttcc agcggatcgc
     1261 cctccctgcc tacctccccg ccttctttat tctataactg cagacaggtg ctgccgtcga
     1321 ggcgcaccac aaaatcccca cggcaggcct gatatgtcat gccgttcagc tccaccggca
     1381 gatcagtggc agaagtgcca tctgcgggca gacctgatgc cgggaccagc ggcagtacga
     1441 cgacttctgc agtacgctgt tctgccagca gagaggctgt gccacggccc ggtcagcttt
     1501 tgtgagttcc acagctagcc gcagctcagg gattaagatt caggtaattt acggaaaaca
     1561 agtgatttcc gtacgggaga gaaggcctaa cgtatattat ctcagaaatg actatgaatc
     1621 cccatatatt gccaattata cagtaaatga ttatcattat catctaatgt gtgcaactga
     1681 tggtctgaaa tagcactttt aggagacaag atgcagtcag ctagtaaatt aatctaccgc
     1741 gggaagttgc ttggcagttt acccactgtg ggcgagattc ataaagaaat cgcagtatcg
     1801 gaagaaaccg taacatggat ctctctgcaa cgagatatta tagcgaatat tttgctggga
     1861 aaagatcctc gcttacttgt gattgttggt ccatgctcaa tccatgacgt tcaggccgca
     1921 gttgattatg cgaagcgatt gttcgtatta cagaataagt acttatcgaa aatgtacatt
     1981 gtaatgcgca cttattttga gaaacctcga acccgtaaag gctggaaggg aataatgcac
     2041 gatcctgatc taaatggaag ttatgatgtt gagaaaggga tacgatatgc tcgccagtgt
     2101 ttgtcttcaa tcacaacgat gcgtgtcgca accgctacag aattcctcga tccttttctt
     2161 actccctata ttgctgattt aatatgttgg ggtgctattg gtgccagaac cactgaatca
     2221 caaacgcatc gtcaacttgc gtctggttta cattgtccag ttggatttaa aaacagtact
     2281 gatggaaata ttaacttagc tattgacgct attattgcag cccgtgaaca acatattgta
     2341 tatatgacat ctttaacaaa tagtatatct acactattaa ccgatggcaa tctacatgga
     2401 catctaatat tacgtggagg tagagaacct aactatggtt tgtctgatat aactaaggca
     2461 gtgaaattaa tgcatgatga gggcattaac catcgtctga taattgattg tagccatggt
     2521 aatagcggta aagtggctaa acggcaaatt tccgttgcac gtcaagttat agataatcgg
     2581 aaaaaaatac caggatatgt agctggcatt atggtggaaa gctttttaca agatggaaaa
     2641 caatccgata gttttccgct agagtatggt cagtctgtta ctgatgaatg catatcatgg
     2701 caacagacag aacaattatt atcaactctg gcagcacagc tttaacttca actattgcac
     2761 atatattgct tttaaagggg gaaaaatgaa attacttagt ttaagacact caattagtgt
     2821 tatcagagaa gcagatattt tatctctata gtttatgatt atataaagcg ctatcgtagc
     2881 cgcgtattta atggtcttaa aatataaaac aattggttac tatctatggc aagctcctga
     2941 attatgtgtg tgaaccagga gtttctcagg aatttcctca caatgtggca ctatcactgt
     3001 cacctttttc tccttggaaa cctccaggga tgccccaaag tttcggtcag tttttagata
     3061 ggtagcattc ctcattaata atgaggacac cgagggtaga atcttaactc cgtgttaata
     3121 gaataaaaaa caatcatcag aactgagtag ggaaagcgaa tcatgatgta tgaagtttca
     3181 cacacaatgt gaatcaaaaa cgctcgtaga taatccgata gtgctgcctg atacgctgaa
     3241 agaaattgtt cgcaggaaga tctgattatg ctgattcgtc gaccacaggc ccttatatac
     3301 ataagcagcg cccgccaggt cagaaacaat gtgaacacta tccgtgagaa attgtgacaa
     3361 aattcccgat gatcgacaag tgcaatgaaa t
//
LOCUS       DAMZII010000255         3354 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZII010000255 DAMZII010000000
VERSION     DAMZII010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.259"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            47..1927
                     /locus_tag="POA77_004788"
     CDS             47..1927
                     /locus_tag="POA77_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDI6368099.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSVQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADVADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMIK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSAFVNDKFIEQVNKLI
                     GI"
     gene            complement(1945..2292)
                     /locus_tag="POA77_004789"
     CDS             complement(1945..2292)
                     /locus_tag="POA77_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDI6368100.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDSQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            2411..2758
                     /locus_tag="POA77_004790"
     CDS             2411..2758
                     /locus_tag="POA77_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368101.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLCILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYRGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWGDV"
     gene            complement(2776..>3354)
                     /locus_tag="POA77_004791"
     CDS             complement(2776..>3354)
                     /locus_tag="POA77_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368102.1"
                     /translation="RRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEAPVYG
                     PNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLTEQDRLRAEQRAAEV
                     VVLPLVPAAGLPADGTSATDLEVELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVR
                     REGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
BASE COUNT          927 a          775 c          838 g          814 t
ORIGIN      
        1 agcagttaat tcatttgttt tcctcagagg atgaaggaga taccgaatgt ctgaccctgt
       61 acgtattaca aatcccggtg cagaatcgct ggggtatgat tcagatggcc atgaaattat
      121 ggccgttgat atttatgtaa accctccacg tgtcgatgtt tttcatggta ccccgcctgc
      181 atggagttcc ttcgggaaca aaaccatctg gggcggaaac gagtgggtcg atgattcccc
      241 aacccgaagt gatatcgaaa aaagggacaa ggaaatcaca gcgtacaaaa acacgctcag
      301 cgtgcagcag aaagagaatg agaataagcg tactgaagcc ggaaaacgcc tttctgcggc
      361 aattgctgca agggaaaaag atgaaaacac actgaaaaca ctccgtgccg gaaacgcaga
      421 tgtcgctgat attacacgac aggagttcag actcctgcag gcagagctga gagaatacgg
      481 attccgtact gaaatcgccg gatatgatgc cctccggctg catacagaga gccggatgct
      541 gtttgctgat gctgattctc ttcgtatatc tccccgcgag gccaggtcgt taatcgaaca
      601 ggctgaaaaa cggcagaagg atgcgcagaa cgcagacaag aaggccgctg atatgcttgc
      661 tgaatacgag cgcagaaaag gtattctgga cacgcggttg tcagagctgg aaaaaaatgg
      721 cggggcagcc cttgccgttc ttgatgcaca acaggcccgt ctgctcgggc agcagacacg
      781 gaatgacagg gccatttcag aggcccggaa taaactcagt tcggtgacgg aatcgcttaa
      841 gacggcccgt aatgcattaa ccagagctga acaacagctg acgcaacaga aaaacacgcc
      901 tgacggcaaa acgatagttt cccctgaaaa attcccgggg cgttcatcaa caaatcattc
      961 tattgttgtg agtggtgatc cgagatttgc cggtacgata aaaatcacaa ccagcgcggt
     1021 catcgataac cgtgcaaacc tgaattatct tctgacccat tccggtctgg actataaacg
     1081 caatattctg aatgaccgga atccggtggt gacagaggat gtggaaggtg acaagaaaat
     1141 ttataatgct gaagttgctg aatgggataa gttacggcaa cgattgcttg atgccagaaa
     1201 taaaatcacc tctgctgaat ctgcggtaaa ttcggcgaga aataacgtca gtgccagaac
     1261 aaatgaacaa aagcatgcaa atgatgctct taacgccctg ttgaaggaaa aagagaatat
     1321 ccgtagccag cttgctgaca taaatcagaa aatagctgaa gagaaaagaa aaagggatga
     1381 aataaatatg ataaaggatg ccataaaact cacctctgat ttctacagaa cgatatatga
     1441 tgagttcggt aaacaagcat ccgaacttgc taaggagctg gcttctgtat ctcaagggaa
     1501 acagattaag agtgtggatg atgcactgaa cgcttttgat aaattccgta ataatctgaa
     1561 caagaaatat agcatacaag atcgcatggc catttctaaa gccctggaag ctattaatca
     1621 ggtccatatg gcggagaatt ttaagctgtt cagtaaggca tttggtttta ccggaaaagt
     1681 tattgatcgt tatgatgttg ctgtggagtt acaaaaggct gtaaaaacgg acaactggcg
     1741 tccatttttt gtaaaacttg aatcactggc agcaggaaga gctgcttcag cagttacagc
     1801 atggacgttt tccgtcatgc tgggaacccc tgtaggtatt ctgggttttg caattattat
     1861 ggcggctgtg agtgcgtttg ttaatgataa gtttattgag caggtcaata aacttattgg
     1921 tatctgacac ataatgatgc cctttcattt tcctgaaagg gctttgcaga atataaaaat
     1981 taagtaaatg gcaaccacag gaactgatat aacgaaacag aataattcaa atattgcagt
     2041 taagcttccg ccaactggat ttgtaaagaa gtctttttgc cagaattctt tccctgtaaa
     2101 ccttattacc attttctgta ttatcctcat tgaaaaagga aaagtaatgg cactgataat
     2161 gcatccgatg aacgcataaa acattcttgc attttcctgg ctatctttcc atcccagata
     2221 aaaaactgta agaataatga ggcttggtat caggctcttc agtaaatact ttacagatat
     2281 atccagtttc atctgttttc tcctttattg ttatcgtgta ccggtgcgtg ataacataat
     2341 cacagcatga taataataat caataacaat aagctgtgtc acgtttacat catggaacgg
     2401 aggtgctcct gtgacctttg agcagaaaaa agcccgggcc attgccctga tggacagcaa
     2461 aaagatgtgg cggagcaatt atgcccctcc tcttctgtgt attctctggc ggctgggtat
     2521 ccggcttccg ccattaccct ttatgccgtt ctggcaggtc acgctgttga tggggagctt
     2581 atggggcatt tcctggggat gtgcaatgtg gtttatctac cgggggccgt caggaatggt
     2641 ggcaggtgag gccataatta taagtatcac gggtggtttt ttgttcggac tgctcacggc
     2701 ctctttccac tggtggcgca ggaaagttaa ccggctgcca ccatggggtg atgtgtaaac
     2761 cgtgctggca tgtttttacg gagtgacgct ttcattaacc tgtacacgaa cttctattcc
     2821 ggcatcatga caggcctgca gccactgcgc cacttccagc ggatcgccct cccggcgtac
     2881 cactctgcct tctttattcc ataactgcag acaggtgctg ccgtcgagac gcaccacaaa
     2941 atccccacgg caggcctgat acgttatgcc gttcagctcc acctccagat cagtggcaga
     3001 agtgccatct gcgggcagac ctgctgccgg gaccagcggc agtacgacga cttctgccgc
     3061 acgctgttct gcccggaggc ggtcctgttc tgtcagcagc agaggctgtg ccacggcccg
     3121 gccggcgtcc gtcagctcca ctgccagctg caggttcgga gcgcgcaggg tacgcagcca
     3181 gccggcatcc tccagacgac ggcaggaggc gcgcaggttt ggcccgtaga ccggggcttc
     3241 cccgcttcgc tccagcaccc gttctatgtc ccgcgtcagc acgggtcctg gccgtttttc
     3301 atcaagggca gccagcacaa tcagcactct ccgctgcagt ggcgacggtc ggcg
//
LOCUS       DAMZII010000256         3335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZII010000256 DAMZII010000000
VERSION     DAMZII010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.260"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..517
                     /locus_tag="POA77_004792"
     CDS             <1..517
                     /locus_tag="POA77_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDI6368103.1"
                     /translation="PTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFL
                     HNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTSV
                     EGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGTS
                     GADDWRAPLEA"
     gene            727..2868
                     /locus_tag="POA77_004793"
     CDS             727..2868
                     /locus_tag="POA77_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI6368104.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            2944..>3335
                     /locus_tag="POA77_004794"
     CDS             2944..>3335
                     /locus_tag="POA77_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368105.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPL"
BASE COUNT          811 a          876 c          977 g          671 t
ORIGIN      
        1 gccaacaccg tgttatatct ccatcactgg tcagacccag ggaaatatta ctgcaggtgc
       61 atttaccgct gattctgtcg gcaacatcta tgtgcagggg cacgaagacg agatgctggt
      121 gcaggaattc ctgcataacg ttactgtccc gaccgatcct cagtccggcc agcctgcggg
      181 tcagcgtgct cacaagccgt tcatttttac cgtggcgctg aacaaagctg ttccgctgat
      241 gtacaacgca ctggcctccg gcgaaatgct gccaaccact gaacttcact ggtggcgtac
      301 ctctgtggaa ggcaaacagg agcattattt caccacccgc ctgaccgatt cgacgattgt
      361 cgatatgaaa ctgcatatgc cgcattgcca ggatccggcg aaacgcgagt tcacccagtt
      421 gctcgaagtt tcactggcct accgcaaaat agagtgggaa cacgtcaagt ctggcacctc
      481 cggtgccgac gactggcgcg caccgctgga agcataagtt aagccaacag catccggcta
      541 tcaggccgga tgctgttttt gtattaagcc gtaaaaagaa aggttgtcag taaggggtta
      601 cgttcacggg agatggcgga tgccggaaac ttcggcggta gcgtgcctga acgcaagcgt
      661 aacactgtac tgtcacagcc gctttaacag cgaactgtct gtttttcctt aaccccggag
      721 gttgctatgt caaccggatt acgtttcacg ctggaagtgg acggcctgcc accggatgct
      781 tttgcggtgg tctcctttca tctgaaccag tcactctctt cgctcttttc cctcgatctc
      841 tctctggtca gccagcagtt tctctccctt gaatttcagc aaatactgga caaaatggcc
      901 tacctgacga tatggcaggg cgatgacgta cagcgccggg tgaaaggtgt ggtgacctgg
      961 tttgaactgg gggagaacga caaaaaccag atgctgtaca gcatgaaggt gtgcccaccg
     1021 ctgtggcgca cagggctgcg ccagaacttc cgtatcttcc agaatgagga catcgaaagc
     1081 atcctcgcga cgatcctgaa agaaaacggt gtgaccgagt ggagtccgct gttcagcgag
     1141 ccgcatcctt cccgtgagtt ttgtgtccag tacggcgaaa ccgattacga tttcctgtgc
     1201 cggatggcgg cggaggaagg catcttcttt tatgaggagc acgcgcaaaa aagtaccgac
     1261 cagagcctgg tcctgtgcga caccgtgcgt tatctgccgg agtcctttga gatcccctgg
     1321 aatccgaaca cccgtaccga ggtgagcacc ctctgcatca gccagttccg ctacagcgca
     1381 caaatccgtc cttcttccgt ggtgacaaaa gactacacct ttaaacgtcc cggctgggca
     1441 gggcgttttg accaggaggg ccagcaccag gactaccagc gcacgcagta tgaagtgtat
     1501 gactaccccg gacgtttcaa gggcgcccac gggcagaact ttgcccgctg gcagatggac
     1561 ggctggcgaa acaacgcaga agtggcgcgc ggaacaagcc gttcgccgga gatatggccg
     1621 ggacggcgaa ttgtgctgac ggggcatccg caggcgaacc tgaaccggga atggcaggtg
     1681 gtggcgagcg atcttcacgg cgaacagccg caggcggtac cgggacgcag gggttcaggc
     1741 accacgctgg ataaccattt tgcggtaata ccggcagaca gaacatggcg accacagccg
     1801 ttgctgaaac cgctggtgga cggcccgcag agcgccgtcg tgacgggacc ggcaggcgag
     1861 gaaatcttct gtgatgaaca tggccgcgtg cgggtgaaat ttaactggga ccgttataac
     1921 ccgtcaaacc aggacagttc atgctggatc cgtgtggcac aggcgtgggc aggcaccgga
     1981 ttcggtaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
     2041 gatccggacc agccgatcat tatggggcgc acctaccacc aggaaaaccg cacacccggc
     2101 agcctgccgg gaacaaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
     2161 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
     2221 aagaacatga acaccgaggt gctgaataac cgcaccactg atgtgataaa caaccatgcc
     2281 gaaaaaatag gtaacaacca ggcgatcacc gttaccaata accagatcca gaacattggc
     2341 gttaatcaga tacagacggt tggtgtcaac caggtggaaa cggtgggcag taaccagatt
     2401 atcaaagtgg gatcaaacca ggttgaaaag gtggggatca ttcgtgcgct gacggtgggt
     2461 gtagcttacc agacgacggt aggcggcatt atgaatacct cggtggcgtt gttgcagtcc
     2521 tcacaggtag ggctgcataa atcactgatg gtgggaatgg gctacagcgt caatgtgggg
     2581 aataacgtca ccttctcggt gggcaagacg atgaaggaaa acaccggaca aacagcagtt
     2641 tattctgccg gtgagcatct tgaactctgc tgtggtaagg caaggctggt gctgacgaag
     2701 gacggaagca tatttctcaa cggtacgcac attcatctgg aaggggagtc ggatgtgaac
     2761 ggtgatgcgc cagtgattaa ctggaactgt ggtgccacac aacctgtacc ggatgcgcct
     2821 gtgccgaaag atttaccccc cggaatgccg gatatgcggc aattttgagt caggtgcaaa
     2881 aaattaaaac ggaactggtg cagtgaccag atgaaccata ttaaaagcag aaggaattga
     2941 tttatgggcg gcggaaaacc ggcggcgcgg cagggtgaca tgacccgcaa gggactggat
     3001 attgtgcagg gttcagcagg ggtgctgata ggtgcgccaa ccggtgtggc ctgctcggtg
     3061 tgtcccacaa aaaaggacag tccgaactat ggcagcccgg tgaacccgct actcggtgcg
     3121 aaggttctgc ctgttgaaac cgacctggca ctgcccggcc cgctgccgtt tattcttttc
     3181 cgcgcctaca gcagctaccg gaccaggacg cccgcgccag tgggcgtgtt tggtcccggc
     3241 tggaaagcgc ccttcgatat ccgcttacag atacgcgatg aaggcctgat actcaacgac
     3301 agcggcggca ggagcattca ctttgagccg ctgtt
//
LOCUS       DAMZII010000257         3268 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZII010000257 DAMZII010000000
VERSION     DAMZII010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.261"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            165..1268
                     /locus_tag="POA77_004795"
     CDS             165..1268
                     /locus_tag="POA77_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HDI6368106.1"
                     /translation="MPLAQLKRDTLSLVKLNGDRTDGIKGSVQKNKIFISRSDIAIEK
                     GDLLIRSMPHGGTEEYIVIEPNFRMGVGGIPATYQAEVILKENTTTEEMTSSPSTAVP
                     EAVIATVNTFIALCVTIVNEGASSPDKYASQFYRLRQELLELDNIITIPSWIRFSMSP
                     ADIKNNVGLNVTGGDGAWGRRRQYLQSESIKILSSYKTAPIIEHSVSENKIKNTEQVS
                     GLTTMASDAGLNMKKKVFIVHGHDDKLKNEVYIFLANEGFQPVILHHEANEGQTIIEK
                     LEKHIDTVSYAVVLYTACDEGKAKNETELKKRARQNVVLEHGWLMSKLSRKFVAAIVE
                     DGVEFPGDLSGVVRISASDWKYDLSKELKVLNN"
     gene            1298..2428
                     /locus_tag="POA77_004796"
     CDS             1298..2428
                     /locus_tag="POA77_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HDI6368107.1"
                     /translation="MGYTRNQHMLSQWFLRNFRSDDTAQSPKEKQRVWAHVVVPTAEG
                     KNDIKDIPLPISSVAVCKDCFRLTDGDTGEVFDIEHELSDYEQDMALLVRDLVQNHNF
                     ARLANCDTDDFPVEKLASFAIFQMLLNLNNPQSRFPGKNELFQSFINPVKNNLQHIIS
                     ETISLSDVMPELAALSIYQKLIRIARSSSGDDEKAKAMFVLFSLLALQGKITMIDTMA
                     WLRGEVFSGIHRVDIFHTGHHFYSTEPRPVFTVSPNVFCKMTEERVLYLPLAHNLALK
                     FYQYPEHGFFTPLEINVFSPDPQKLLTKDMSRIKVYKCSYDYIDQVMSTIDMYNVGFS
                     NIIYSSWQLSDVENYLRLQNEHHDTYYLPEHPVCWTVSREKG"
     gene            2428..2718
                     /locus_tag="POA77_004797"
     CDS             2428..2718
                     /locus_tag="POA77_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="korC"
                     /protein_id="HDI6368108.1"
                     /translation="MALTPEQISCRQQLVAMGDFNAHTLLPGEEWTRPENADVRHVLS
                     LIPLTDIQLANRLDVDERTIRKWKSGETSMVFTTWCCLCWLAGLGMLLEESA"
BASE COUNT          920 a          702 c          754 g          892 t
ORIGIN      
        1 cttttctgcc gcgcagatcc ggtttccggc taacacggag ataataggtt aggtggttct
       61 gttgttaggg tatggagaac atttctcgag ccgagcaagg gaaattactc acaccatttg
      121 ctatgctaac ggccacacta accagccttt tttcggggta atacatgccg ttagcacaac
      181 tgaagcgtga cactctttcc cttgttaagc taaatggcga caggactgat gggataaaag
      241 gaagtgttca aaagaataag attttcatta gccggagcga tattgctatt gaaaaggggg
      301 atctgcttat acgcagtatg ccgcatggcg gtactgaaga atatattgta atcgaaccta
      361 acttcagaat gggtgtaggc ggtattcctg cgacctatca ggctgaggtg atactgaagg
      421 aaaatacaac taccgaggaa atgacatcat ccccttccac agcagtaccg gaagcagtta
      481 ttgccactgt aaatacattc attgctctgt gtgtcaccat tgtaaatgag ggcgcatcaa
      541 gtcctgataa atatgcttcg cagttttatc gcttgcgtca ggaattactt gaactcgata
      601 atattatcac tattccttca tggatcagat tctccatgtc acccgctgac attaagaata
      661 atgttggttt aaacgtcaca ggtggcgatg gagcttgggg gcgaagacga cagtatttac
      721 agagcgaatc tattaaaata ctttcaagct ataaaaccgc accaattatt gagcattctg
      781 ttagtgagaa taaaataaaa aatactgagc aagtcagcgg gttgacaacg atggcaagcg
      841 atgcagggtt aaacatgaag aagaaagtat tcattgtcca tgggcacgat gataaactaa
      901 aaaacgaggt ttacattttt ctggcaaatg aaggtttcca gcctgtcata cttcatcatg
      961 aagccaatga aggacagacc ataatcgaga agcttgagaa gcatatcgac actgtatcct
     1021 atgcagtggt gctttatact gcctgtgatg aagggaaagc caagaatgaa actgaactaa
     1081 aaaaaagagc ccggcagaat gttgttttag aacacggctg gctcatgtcc aagctatcgc
     1141 gtaaattcgt cgctgcgata gttgaagatg gcgttgaatt tcctggagac ctgtccggag
     1201 tggtcagaat atctgcatca gactggaaat atgatctgtc taaagaatta aaagtgctca
     1261 ataattaaat tgtattcagt gatgtaagga tactattatg ggatacacca gaaatcagca
     1321 catgctctca cagtggtttc tcaggaactt ccgcagtgat gatacggcac agagtccgaa
     1381 ggagaagcaa cgggtctggg cccacgttgt ggttcctacg gcagaaggca aaaatgacat
     1441 aaaggacatc ccgctaccga tatccagcgt ggccgtctgt aaggactgct ttcgtcttac
     1501 cgacggtgac acaggcgagg tttttgatat cgagcatgag ctcagtgatt atgaacagga
     1561 tatggctttg cttgtccggg atctggttca gaaccataat ttcgcgcgtc tggctaactg
     1621 tgacactgat gattttccgg tagagaagct tgcaagtttt gctatatttc agatgttact
     1681 taacctcaat aacccgcaaa gcagatttcc gggtaaaaat gagttatttc agtcattcat
     1741 taacccagta aaaaacaacc ttcagcatat catttccgaa acaatttctt tgtctgacgt
     1801 tatgccggag ctggcggctt taagtatcta tcagaaactg attcgtatag ctcgttcttc
     1861 ttctggggat gacgaaaaag ctaaggccat gtttgtatta ttttcccttc tcgcacttca
     1921 gggtaaaata acaatgattg acactatggc ctggctacgc ggagaggttt tttccggaat
     1981 acatcgtgtg gatatatttc ataccgggca ccacttttac agtacagaac cacgtccggt
     2041 atttacagta tcacctaatg tattttgcaa aatgacagaa gaacgcgtcc tgtaccttcc
     2101 ccttgctcat aatttggctc ttaagtttta tcagtacccg gaacacgggt ttttcacccc
     2161 actggaaatt aatgtattca gcccggaccc tcagaaacta cttacaaaag acatgtcacg
     2221 aatcaaagtt tataaatgct cctatgatta tattgatcag gttatgtcaa caattgatat
     2281 gtataacgtc ggattttcaa atattattta ttctagctgg cagctcagtg atgttgaaaa
     2341 ttatctgagg ctacagaacg aacatcatga tacttattat cttccggaac atcccgtttg
     2401 ctggactgta tccagggaga aagggtgatg gccctgaccc cggaacagat aagttgccga
     2461 cagcagctcg tggcaatggg tgacttcaat gcccataccc tgctgcccgg agaggagtgg
     2521 accaggccag aaaacgctga tgtccggcac gttctgtcgc tgatccccct gacggacatc
     2581 cagctggcta accggctgga tgtggacgaa cgcaccatcc gcaagtggaa atccggcgag
     2641 accagtatgg tattcaccac ttggtgctgc ctgtgctggc tggccgggct gggaatgctg
     2701 ctggaagaat cggcttagcc ggttctgatg cgtttccaga ttcttttgag tatgaagcgt
     2761 cgctgatatc gccattgtga acggtagata tgggggagtc gatcatagtt cttccaaaac
     2821 aaggtccagc ctttataggc accaacaagt agggcaatga gagagccagt ttttaagagg
     2881 ccaaagaaaa cggccatgag aaatacagtc atacgatgtt ccttatgtaa aactgtattt
     2941 aggaaatccg aagcggcctt taaggccggt tgatgaaaat gaactgaaaa aagtttctct
     3001 ggcctatttg ttgccatttc aggctagctc tttcgtgaat tttgcctctc aggcaataat
     3061 ccactcatca caacaattca ccgtttttag tgcttatcct gcaaaaatct gtttttttcc
     3121 cttcctgaga tccgaaaact cattgtcacg gttcttcctc tccctccgca cagcgttaca
     3181 tcccgtcagc acagtatgta gtgcctcgta cagttgccca tggcactata tgttgtgttg
     3241 tatctctgga ctgtgatgcg ccgcgcag
//
LOCUS       DAMZII010000258         3238 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZII010000258 DAMZII010000000
VERSION     DAMZII010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.262"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            106..1071
                     /gene="dusB"
                     /locus_tag="POA77_004798"
     CDS             106..1071
                     /gene="dusB"
                     /locus_tag="POA77_004798"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDI6368109.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1097..1393
                     /gene="fis"
                     /locus_tag="POA77_004799"
     CDS             1097..1393
                     /gene="fis"
                     /locus_tag="POA77_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDI6368110.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1479..2223
                     /gene="yhdJ"
                     /locus_tag="POA77_004800"
                     /pseudo
     CDS             1479..2223
                     /gene="yhdJ"
                     /locus_tag="POA77_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
     gene            2307..2486
                     /locus_tag="POA77_004801"
     CDS             2307..2486
                     /locus_tag="POA77_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDI6368111.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2489..3151)
                     /gene="acrS"
                     /locus_tag="POA77_004802"
     CDS             complement(2489..3151)
                     /gene="acrS"
                     /locus_tag="POA77_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDI6368112.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          883 a          741 c          772 g          842 t
ORIGIN      
        1 ctttgatcca tctcagagga ttggtcaaag tttggccttt catctcgtgc aaaaaatgcg
       61 taatatacgc cgccttgcag tcacagtatg gtcatttctt aactcatgcg catcggacaa
      121 tatcagctca gaaatcgcct gatcgcagcg cccatggctg gcattacaga cagacctttt
      181 cggacgttgt gctacgagat gggagccgga ttgacagtat ccgagatgat gtcttctaac
      241 ccacaggttt gggaaagcga caaatctcgt ttacggatgg tgcacattga tgaacccggt
      301 attcgcaccg tgcaaattgc tggtagcgat ccgaaagaaa tggcagatgc agcacgtatt
      361 aacgtggaaa gcggtgccca gattattgat atcaatatgg gttgcccggc taaaaaagtg
      421 aatcgcaagc tcgcaggttc agcccttttg cagtacccgg atgtcgttaa atcgatcctt
      481 accgaggtcg tcaatgcagt ggacgttcct gttaccctga agattcgcac cggctgggca
      541 ccggaacacc gtaactgcga agagattgcc caactggctg aagactgtgg cattcaggct
      601 ctgaccattc atggccgtac acgcgcctgt ttgttcaatg gagaagctga gtacgacagt
      661 attcgggcag ttaagcagaa agtttccatt ccggttatcg cgaatggcga cattactgac
      721 ccgcttaaag ccagagctgt gctcgactat acaggggcgg atgccctgat gataggccgc
      781 gcagctcagg gaagaccctg gatctttcgg gaaatccagc attatctgga cactggggag
      841 ttgctgcccc cgctgccttt ggcagaggtt aagcgcttgc tttgcgcgca cgttcgggaa
      901 ctgcatgact tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca cgtttcctgg
      961 tatctccagg aacacgctcc aaatgaccag tttcggcgca cattcaacgc cattgaggat
     1021 gccagcgaac agctggaggc gttggaggca tacttcgaaa attttgcgta aacagaaata
     1081 aagagctgac agaactatgt tcgaacaacg cgtaaattct gacgtactga ccgtttctac
     1141 cgttaactct caggatcagg taacccaaaa acccctgcgt gactcggtta aacaggcact
     1201 gaagaactat tttgctcaac tgaatggtca ggatgtgaat gacctctatg agctggtact
     1261 ggctgaagta gaacagcccc tgttggacat ggtgatgcaa tacacccgtg gtaaccagac
     1321 ccgtgctgcg ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat tgaaaaaata
     1381 cggcatgaac taattcaggt tagctaaatg cttgattaaa aaggcgctac tcggcatggg
     1441 gaagcgcctt ttttataggt gtcacaaagg gagtgaccat gagaacagga tgtgaaccga
     1501 cccggtttgg taatgaagct aagaccatta ttcacggtga tgcccttgcc gaacttaaaa
     1561 agctacctac tgaaagcgtc gatctgatct ttgccgaccc accgtataac atcggtaaaa
     1621 attttgatgg tctgatcgaa gcctggaaag aagatctgtt tatcgactgg ctgtttgaag
     1681 tgattgcaga gtgccaccgc gttctgaaaa agcagggcag catgtacatt atgaacagta
     1741 cggaaaacat gccctttatc gatctccagt gccgcaagct ttttaccatc aaaagtcgca
     1801 tcgtctggtc atatgacagt tctggagtac aggcgaaaaa acactacggc tccatgtacg
     1861 aacccatcct gatgatggtg aaagacgcaa agaactacac attcaacggt gatgctattc
     1921 tggtagaagc caaaaccgga agccttactg aaacgcatta ttctcgcctc ttccaaccca
     1981 ggcgatatcg ttctcgaccc gtttgccggt agctttacta ccggtgccgt agccgtctcc
     2041 agcgggcgaa aattcattgg tattgagatc aacagcgagt acatcaaaat ggggcttcga
     2101 cggctggatg tcgcgtcgca ttactctgcg gaagaactgg cgaaagtgaa aaaaagaaag
     2161 accggcaacc tgtcaaaacg aagccggtta agcgaagttg accccgatct cattgcaaag
     2221 taaaggaagt gtaagcctgg ttttcagatt attcatttcg tgtatatttc ctgccagact
     2281 tggttaaaca tgcacaggct caggtaatga ttcgcaagta ttggtggctc gtcgttttcg
     2341 ctgtcttcgt ttttctgttt gatactttac tgatgcagtg gattgaactg ctgacaacag
     2401 aaacagacaa atgccgcaat atgaactcag ttaatccact aaaactggtt aactgtgacg
     2461 aactgaattt tcaggacaga atgtgaattt acatgacact taattcattc gtttgatgaa
     2521 ttaatttcgt tatgttttca tctggcatga acattcttaa tacgttatcg accagagcgg
     2581 gggcttgttt ataaagatca taacccgcca tattcattaa ccagttttga acaattccgc
     2641 tgaaggcacc attaataata atcatcacaa catctaaatc gaggttattt gctacacaac
     2701 cttgttgctg acacgcctgc aatacttcgc ggagagtctg cggattgaag cccatctttt
     2761 cgcgtatcac tccctcggcc agcatctcat cattaaattc acatttgtga tataagattt
     2821 tcagcaacgc ctgctggcgg ggaattttgg caatatattg caagccgaca atcaattttt
     2881 cacgcaattg ttgaaatggg tcatgctcta acccagccgt caagtgatcc tggattaact
     2941 ctcgcaatga aggctgttgc aaccacatct cattaaacag ttgagtcttg ttttcgaagt
     3001 gccagtagat agcgccacgc gtaacgttag cagcgtcggc aatgtcgttg agtgtcgtct
     3061 tgcttacgcc atgctgcgca aactgggcga tggcagtttc aatcagttct tgccgggtct
     3121 tcagagcttc ggctttggtt ctttttgcca tgattaatta ttcaggaaat aaatatattc
     3181 gacacagagt gagaaaatag cgaaggttaa tctatcacct aatgtgtatt tatacgag
//
LOCUS       DAMZII010000259         3201 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZII010000259 DAMZII010000000
VERSION     DAMZII010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.263"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            78..287
                     /locus_tag="POA77_004803"
     CDS             78..287
                     /locus_tag="POA77_004803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368113.1"
                     /translation="MVFNSLIRQTMGNAGRVSWHLTPLIARAWREAGRVPWHLTPLIA
                     RPWGGWSRSVAFNPLNLQAMGKLVS"
     gene            complement(409..555)
                     /locus_tag="POA77_004804"
     CDS             complement(409..555)
                     /locus_tag="POA77_004804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368114.1"
                     /translation="MPRYATSFSMAWRLRGLNATLRDLLPHSLAIKGVKCHAKRPASP
                     WPGD"
     gene            601..747
                     /locus_tag="POA77_004805"
     CDS             601..747
                     /locus_tag="POA77_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368115.1"
                     /translation="MAFNPLNRQSMAGSWSGTVAFNPLNRQAMEECWSRTMAFNPLNR
                     QAMG"
     gene            1045..2298
                     /locus_tag="POA77_004806"
     CDS             1045..2298
                     /locus_tag="POA77_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI6368116.1"
                     /translation="MNLIDKIALVGQRMKSEQISLKESLLVSSRVSVSDDSVDGVDRL
                     IYNHCLNKKNLSDFFGKSRVTFNKILADLEEKELVGAPIYQNKNHLYTRWDVQKIMDA
                     LNYPRYSDYYCSRTIVTQNHKGGTGKSTTSGVLAVAAALDLHLNARILLIEWDPQGSI
                     GSGMIQSVAEDDVFLTAIDAILGVYEEDSDYRKYLDLGYSEEQIIEGMPFSTHLPNLD
                     VITAFPTDARFKDKYWQCSREERTELLLRFKEVILPVLKSKYDLIIIDTPPEDSPITW
                     AADEAADGILVAVSPREYDYASTTDFMLTISERFKQSPSKGENLSWFKVLAVNVDDKS
                     PYEKIVLDKLVRTVQELFMSANIKNSEAFKAAASRGRTVLDIKKSEELCSPKQLDVAE
                     ESVMAVYQQFINEIKSFSVKQGGNV"
     gene            2295..>3201
                     /locus_tag="POA77_004807"
     CDS             2295..>3201
                     /locus_tag="POA77_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HDI6368117.1"
                     /translation="MSDEQHIGNDKSRYINAPKRTEVIHRSGLQGLKGQPRLKKLFTL
                     HNGRKLEAEHIIVPAEKVELETTVHPLNPRNQEALSVNAVRDILKDIEARGVDTEGVA
                     VKRNGVYLLIEGSRRRFCCIQSAKELPLWVLPDDVNADDINSIISATQTSRRFSYREV
                     GLKYLREMEEHGFVTNEELANYHGISHVSVSKRVQAAKINSTLIALFPDYEAIPNSYY
                     NRLFRLQKYIEKNLFSLEEVVENTREEIRDLDISDIAEAQKTVMEKITTVVEKLDIKP
                     PSKGWDTRELATFTNKDKYARISKSS"
BASE COUNT          920 a          661 c          778 g          842 t
ORIGIN      
        1 ccatggggag gctggtcgcg tagcgtggca tttaatcctt taatcgccag gacatggggt
       61 catgctggtc aggtaccgtg gtatttaact cccttattcg ccagaccatg gggaatgctg
      121 gtcgcgtatc ctggcattta acccccttaa tcgccagagc atggcgggaa gctggtcggg
      181 taccgtggca tttaaccccc ttaatcgcca ggccatgggg aggctggtcg cgtagcgtgg
      241 catttaaccc ccttaatctc caggctatgg ggaagctggt ctcgtagcgt ggcatttaac
      301 ccccttaatc gccagactat ggggaagcag gtcgcgtagt gtggcattta acccccttaa
      361 tcgccaggcc atggagaagc aggtcgcgta gcgtggcatt taacctcctt aatcgccagg
      421 ccatggggaa gctggtcgct tagcgtggca tttaaccccc ttaatcgcca ggctatgggg
      481 aagcaggtcg cgtagtgtgg catttaaccc ccttaatcgc caggccatgg agaagctggt
      541 cgcgtagcgt ggcatttaac ccccttaatc gccaggccat ggggaagctg gtcgcttagc
      601 gtggcattta acccccttaa tcgccagagc atggcgggaa gctggtcggg taccgtggca
      661 tttaaccccc ttaatcgcca ggccatggag gaatgctggt ctcgtaccat ggcatttaac
      721 ccccttaatc gccaggctat ggggtaatgc tggtcgggta ccgtgggatt tacccccctt
      781 attgccacat ctatggagtg atcctgctat gtcggaagat tggtattgtt gatacttaag
      841 aggcttttgg aagacttcaa tgaactgcca attgaaattc aataatgcag atattccaaa
      901 atctaagaaa agaatacaga acgttagcgt aataatagaa ttaaggttac tattaaacgc
      961 ataacgctaa agtatacgca ttatgcgtaa agtttacgta tatacgttta atttcactat
     1021 ttaggtaaaa gggatcggaa gagaatgaat ctaattgata agatcgccct tgtcggtcaa
     1081 cgcatgaaga gcgagcagat ctctctgaaa gagtcattat tggtctcttc gagggtttcg
     1141 gtatcggatg atagtgtaga cggtgttgat cgcctaatct ataaccactg cttgaataaa
     1201 aaaaatctgt cagatttttt tggtaaatct cgcgtaactt ttaacaaaat cttagcggat
     1261 cttgaagaaa aagaactggt tggtgctcct atctaccaga acaaaaacca tctctatacc
     1321 cgctgggatg tccagaaaat aatggatgct ctgaactatc ctcgatatag cgactactac
     1381 tgtagccgta caatcgtaac gcagaaccat aagggtggta cggggaagag tacaacttca
     1441 ggcgttcttg cagttgcggc agcacttgat ttgcatctaa acgcgcggat actgttgatc
     1501 gaatgggacc cacaagggtc tattggaagc gggatgatcc agagcgtagc ggaagatgat
     1561 gttttcctga ctgctattga cgccatctta ggggtttacg aagaagactc agattacaga
     1621 aaatatcttg atttagggta ctcggaagaa caaattattg aaggtatgcc tttttcaaca
     1681 caccttccga atcttgatgt tattaccgca ttccctacag atgcacgttt caaagataaa
     1741 tactggcagt gttccagaga agaacgaact gagttattac tacgttttaa agaggtaatc
     1801 ctgccggtct tgaagagcaa gtacgacctg atcattatcg atactccacc agaagattcg
     1861 ccaatcacat gggcagcgga tgaagctgct gatggcattt tggttgccgt ttcacctcgt
     1921 gagtatgatt atgcctcaac aactgatttc atgttgacga tcagtgagcg attcaaacaa
     1981 tcgccgagca agggcgaaaa tctaagctgg ttcaaagtgt tggcagtgaa tgtcgacgat
     2041 aagagtccct atgaaaagat tgtcctggat aaactggtaa ggacagtgca ggaactcttt
     2101 atgtctgcaa atatcaaaaa ctcagaagcg tttaaagccg cagcttctag gggtagaacc
     2161 gtattggata tcaaaaaatc agaagagctc tgctctccca aacagcttga tgttgccgaa
     2221 gaatccgtga tggcggtcta ccagcaattt attaatgaga ttaagagttt ttcagtgaag
     2281 caagggggta atgtatgagt gatgagcagc atatcgggaa cgacaaatcc cgttatatta
     2341 atgcgcctaa gcgtactgaa gtaatccatc gctccgggct tcaaggcctg aaaggtcagc
     2401 cacgcctgaa gaaacttttt acattacaca atgggcggaa gcttgaggcg gaacacatca
     2461 ttgttccggc ggaaaaggtg gaactggaaa ccacagtaca ccctctgaac ccacgaaatc
     2521 aggaggctct ttctgtaaat gcagtaagag atattctgaa ggacattgaa gcaagaggtg
     2581 ttgatactga gggcgttgca gttaagcgga acggagtgta tctgctaata gaaggtagcc
     2641 gcaggcgttt ctgttgcatt caatcagcaa aagaattgcc attatgggtt ttgcctgatg
     2701 atgttaacgc agatgatatt aattccatca tatcagcaac acaaacttct cgacgctttt
     2761 catatcgtga agtaggttta aagtatttaa gggaaatgga agagcatgga tttgttacca
     2821 atgaagagct tgcaaactat catggtatca gtcacgtgtc cgtctctaag cgagttcagg
     2881 ctgcaaagat taacagcact cttattgcac tgtttcctga ctatgaagct attccgaatt
     2941 cttattacaa tcgcctattc cgacttcaaa aatatataga gaagaattta ttttctcttg
     3001 aagaagttgt tgagaataca cgtgaggaaa ttcgagatct ggatatttct gatattgctg
     3061 aagcacagaa aaccgtaatg gagaagatta caacggtagt tgaaaaactt gatattaaac
     3121 cacctagcaa aggttgggat acacgtgaat tggcaacttt tactaataaa gataaatatg
     3181 caagaattag caaaagttcc t
//
LOCUS       DAMZII010000260         3196 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZII010000260 DAMZII010000000
VERSION     DAMZII010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.264"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            89..1015
                     /locus_tag="POA77_004808"
     CDS             89..1015
                     /locus_tag="POA77_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDI6368118.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(1051..1842)
                     /locus_tag="POA77_004809"
     CDS             complement(1051..1842)
                     /locus_tag="POA77_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDI6368119.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1848..2975)
                     /locus_tag="POA77_004810"
     CDS             complement(1848..2975)
                     /locus_tag="POA77_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDI6368120.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT          868 a          848 c          663 g          817 t
ORIGIN      
        1 acatagatta aataatatct gaaaagcaaa atcactcctg tagaacagta ttataatcac
       61 cgaaaaatat catttaacag gagtgttaat ggctacttta atggaaaaag atgcactttt
      121 aaacggagcc agtcagtgca tcgcgttttt atcaaatatc attgataatt gctcagtttc
      181 gtcgcatcag gattcaggcg atgctctcaa gcgattagtc agctatcgcg attatcttta
      241 ttctaccccc gctgaacttg ttgattttac acaaggtaag attctccttc agcaggtaag
      301 aactcaatat cagcatgaat tcaacaatac tacgcattca gaaaataaag catcttttga
      361 ttctatttgg cagcgtctga caaatcatga agtcacaccg caacagcatc ccattggttt
      421 tgttttaggt gggcaacccg gagcaggtaa atcatctctg attgaattag ccaaaagaga
      481 gacaaaagat aatataatga ttattaacgg tgatgatttt cgatttctac accctgattt
      541 taatcatatc taccaaaatt atggggatga ttttgttacc catacagcga aattttccgg
      601 cgaaacggtc gaacgtgcaa ttgagagagc gatagtcagt aaactcaaca ttgttgtcga
      661 aggaacattt cgtaatgccg ctacgccgtt acaaacatta aaaaaactca aagatgcagg
      721 ctaccaaaca gaagtcatga taaaaacaac ctcagcggca ttaagctggg aaagcaccaa
      781 tgaacggtat aataaggaca aagaggcggg aaatattgcc cgaaaagtgg ataaaaacca
      841 ccatgatatc gtcaccggtt tgctggcaga aaatgcccgc aaggtgtttg cttcaaacct
      901 ggcagataag tttgcggtgt attcgcgtga gaagatgata tttagttcac aagcggcaac
      961 gaatgacgat attgcaacgc ttatacagaa tgaaatcagc gggaatacac aataataata
     1021 agagctgatg gcgtgaaata acgccgggca tcacgcccgg cgcattaccg tctggtacat
     1081 taagctatcc agttccttaa cgtccccttc tctgctatcc ggtaacagcg gtggaatttc
     1141 cccacgcgcc agttccgcac gaataaacgc atgtaacaat ggacggcgcg gaccatattc
     1201 cgcttctcct gcgcgctgtt tacgttccag taattcgtct atttctgcac gtaacggcgc
     1261 atcaagttcg cttcccgcca gtagctcagc aaagcgcatt ggcggtacgc cttttcccgc
     1321 ttcaacccaa cgcacggcca gcagcggacg cagcacgtaa aagtattttt tcagccgcac
     1381 ttcgtcgcct tgtaaataac cgcgaaagtt tttccgcgcc atcgaataat aatgccaacg
     1441 cgcacgcagc ggagaaaacc acttcggcac cattgcctta agcgccgtta tcgtttcttc
     1501 atcttgttga tagaccacag gagaatccag ccattctatc agcgtcggat tcgcaccttt
     1561 caataatccc agcgccttgc gccactccca accggaaaca tctaactcgt cgtcgatggg
     1621 cagctcaata acatcgcgcg gtgactctac tcgcagatac cattccagcg ggtgaacata
     1681 caaaaaccgt acatcgtagt cgctgtcggg cgaggcaaat ccccagccgc ggctccctga
     1741 ttcacaggcg tacagtactt tcacatcata ccgccgttca atttctttta actgctgcga
     1801 taccctttcg cgcatcgcag cactgactcc atttaatggc atgttactca ccctttgata
     1861 ttaaccaacc catgcagaac ctgatccaga ccgccaaata cagagatgtt atcgatccgc
     1921 tcagccactc tctccagcgt ttctagctct tttaaacgca acgctaccgg attgttctcc
     1981 atgactttgg cggtgttcag taacgagcgc gtcgctgccg tttcttcacg tcggcgaatc
     2041 acattcgcct gcgcagattt ctctgcctca accagctgcg cgaggatatt tttcatatca
     2101 ccaggcagga cgatatcttt cacccctaat gaggcaatct ccataccaaa tggcaacatg
     2161 cgcgatttca cctgttcgct gacaacgtca tcaatgacct gtttatcttc cagcagttca
     2221 tccagcgtac gagtacctac cgcttcacgc agggcaaatt gcaattcccg atacaaatga
     2281 tcaatcggct tcgttaactg actaaatgcc agcaacacgt cgctgtagcg ccagttagcg
     2341 gcgaggttaa tccgcagatt gaccttatct ttcgttaaga tttcttgccc gctgacttcc
     2401 agtacctgca agcgggtgtc aaccacttca gcgtcaacca gatggttaat tttccagtat
     2461 gcggtcagcc ccggcggtaa cagcgcctgc gtttcaccat cgattttcag cacgcctaca
     2521 tgccaggccg gaacctgcac cgttagtatt gcatcgcggc ctttaactgc ccctttgcgc
     2581 cgtggttgca ataccgcatt catcacctcc gtttgtacct ggacttgctg cgtattcatc
     2641 cgcaccagtg tcaaatcatc ctctacacgc cagtacaaac ggcgcgttga tggcggcagg
     2701 atctcctgca aaataccgtc catatacaac gccccggctt caatctccga caagtctgcc
     2761 accaggcaat atttctccac ccaatcaggc tgaaagcggc gcaaataatc cgccagcacg
     2821 tcgggaactt cgctgccatc gagcgtaatc agcaagactt ctggcgtatt taaccagggt
     2881 aaaagatgct cgcctgcatg tagcactttg tagtaatcgc cgtttctgga gagtaatgcc
     2941 agctgatctt ttcttacgct tattttttta atcattatct attccttgct taacagccgt
     3001 cgcggaagca gacacgaagc gacaggggaa ttcccttttg cctgttaccc ggcatcttta
     3061 tcggcgacgt tatcggggat cgctgactgc gcaatccctc actttttatt taataagtta
     3121 aaaccagtat tcatttcagg aatgaatgtg ggaatcgaac ccataattgg cgtcaaaggc
     3181 tgacggaaca cgcccg
//
LOCUS       DAMZII010000261         3137 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMZII010000261 DAMZII010000000
VERSION     DAMZII010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.265"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(94..843)
                     /locus_tag="POA77_004811"
     CDS             complement(94..843)
                     /locus_tag="POA77_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6368121.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(933..1106)
                     /locus_tag="POA77_004812"
     CDS             complement(933..1106)
                     /locus_tag="POA77_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368122.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(1253..>3137)
                     /gene="pdeR"
                     /locus_tag="POA77_004813"
     CDS             complement(1253..>3137)
                     /gene="pdeR"
                     /locus_tag="POA77_004813"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI6368123.1"
                     /translation="TETTEPDRTLQLSAEQAARIREMTVITSSLMMSLTVDESDLSVH
                     LVGRKINKREWAGNASAWHDTPAVARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRF
                     NRLCEDYTGLKEHDVIGQSVFKLFMSRREAAASRRNNRVFFRSGNAYEVELWIPTCKG
                     QRLFLFRNKFVHSGSGKNEIFLICSGTDITEERRAQERLRILANTDSITGLPNRNAMQ
                     DLIDHAINHADNNKVGVVYLDLDNFKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVL
                     ARPGGDEFLVLASNTSQSALEAMASRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGS
                     DSTAIIRHADTAMYTAKEGGRGQFCVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHY
                     QPKITWRGEVRSLEALVRWQSPERGLIPPLDFISYAEESGLIVPLGRWVILDVVRQVA
                     KWRDKGINLRVAVNISARQLADQTIFTALKQVLQELNFEYCPIDVELTESCLIENDEL
                     ALSVIQQFSQLGAQVHLDDFGTGYSSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLV
                     RAIVAVAQALNLQVIAEGVESAKEDAFLTKNGINERQGFLFAKPMPAVAFERWYKRYL
                     KRA"
BASE COUNT          845 a          756 c          726 g          810 t
ORIGIN      
        1 aataacaggt aaatagtctt aattatcaac caggaatcat cttagagcgg atgatttgcc
       61 aaactgcaaa tcatccgtag agaagggaaa tggttaaatg tcaacgacgt gaacggtgag
      121 tttactatgc tcaagatgca tcagatcgct ggcgcgtatt ttcgaatcgg taatcacgcg
      181 attgaatcgc tcaacgggac cgatggagta tgaatgtaca gcaccaaatt tcgagctgtc
      241 agtcaggact atcgcttcgc actctttttc cagcacggca ttgaccacat cggtacgcat
      301 catatcgcga ccggtgaatc cagtttcagg ttgccagcca tcaataccaa taaatgcttt
      361 gctgaaatgt acctgctgga tgcactggcg tgtcaaaggg ccaaccatac tttcgctttt
      421 tttctggtac acgccaccga gcaaaatgac ttcacaaggc gcgtctttca gcaaatgcgc
      481 aatgtagctg ctgactgtga tgatagtgac atttttcttc tgttcgccca gagtccgggc
      541 gagcagggca ttgctgctac cattttcgat aaagatggtt tcgcccggtt gaaccagtga
      601 cgcggcaaac tcggcgagtt cacgcttcag cgtatagttg ctcatcatac gggtttcgac
      661 gtcatcacta tcaagcgaaa ctgcaaagcc atgtgcacgg cggaggtaac tcagtttttc
      721 gagggtgttg agatcttggc gaatggtaac ttcagaaact ccagtggctt ttgccagatc
      781 ggttacgcta acctgaccct ggtcaatgac catctgtaga atagtttgtt gtcgggaatt
      841 catcgctgta attcttataa cgttataata ctaaataaaa aaatactgac gcgccgggca
      901 tcctcaaata ggaaaacctg aaggcacgat tgttaaatct cccacggtgc cttcgggatg
      961 tctggcgtag attcagcaac ctggcgcaag agttctgctt taagtatctc gagattatgg
     1021 atcgcagagt ggtaatcgcc tgccacaagg atatctaaca caatatcaat acgttcggca
     1081 acgcgctggg catcaaattc agacatcaag gacatcctta tagcaaaagt ggaaaatgaa
     1141 taatgcaaaa attgccgcta aaagagaagt gtttaacagc aacggctaat tatcatccag
     1201 gaatacgata tataatgacg ggatatagcg ctaagtatat atattcatct acttatgcgc
     1261 gcttcagata gcgtttatac cagcgttcga aggcgacggc gggcatcggt ttggcaaaca
     1321 aaaatccttg ccgctcattg atcccgttct tggttaaaaa agcatcttcc tttgcactct
     1381 ctacaccttc ggcgatcacc tgaagattca atgcctgggc cacagcgacg atcgcccgga
     1441 ccagtgactg cgagacaggt tgtttgtgaa tatctcgaac aaaaacctgg tcaagtttga
     1501 tggcatcgat cggaaagcgc gccagttgcg aaagtgaaga gtagccggta ccaaaatcat
     1561 ccagatgcac ttgcgcacct agttggctaa attgttgaat aacagacagt gccagttcat
     1621 cattctcaat cagacaactc tctgtcagtt caacatctat agggcagtat tcaaaattga
     1681 gttcctggag aacctgtttc agggcggtga aaatggtttg atcggcgagc tgacgtgcag
     1741 aaatatttac cgccactcgc aggtttatgc ctttatcccg ccactttgcc acctggcgta
     1801 cgacatcgag aatcacccaa cggcctaaag gcacaattag ccctgactct tcggcgtagg
     1861 aaatgaagtc caacggtgga atcaacccac gttcaggtga ctgccaacgt actagtgctt
     1921 ccagactgcg cacttcgcca cgccaggtga ttttcggttg atagtgaata accaactgat
     1981 cgttttccag tgctttacgc aagttggtat ccagccagag atattcaaat acccgttgat
     2041 tcatttctgg ggtaaaaacg caaaattgtc ctcgtccgcc ttccttcgct gtgtacattg
     2101 ctgtgtcggc gtgacgaata atagccgtgc tgtctgaacc atgttcggga gagagtgcaa
     2161 tacctactga acagctggta taaacttcaa ttaaaccaat gcgaaagggg agccgtaagc
     2221 gggtcaaaat tcgtgatgcc attgcttcca gcgcgctttg tgaggtgttg gatgccagta
     2281 ccagaaactc atccccacct ggacgcgcca acacctggtc atgttcgaga cagcttaaaa
     2341 tagccaatga cacgtcgcgt aataactggt caccaaacaa atgcccatag gcgtcgttga
     2401 cctttttgaa attatccaaa tcaagataca caaccccaac tttattgtta tctgcatgat
     2461 taatagcgtg atcgattaaa tcctgcattg cgttacgatt cggcagtccg gtgatactgt
     2521 cggtatttgc cagaatacgc agtcgctcct gagcgcggcg ctcttcggta atgtcggtgc
     2581 cggaacagat taaaaaaatc tcgtttttgc cactgccgct gtggacaaat ttattgcgaa
     2641 acagaaacag ccgctggcct ttacatgttg gtatccacag ttcgacttca tatgcattgc
     2701 cgcttcgaaa aaatacacgg ttattgcgcc tggatgccgc agcttcacga cggctcataa
     2761 acagtttaaa cacgctttgc ccaatgacgt cgtgttcttt caaccctgtg taatcttcac
     2821 ataaccgatt gaagcgttgg atattccccc ggctgtcgag aatcactatt gcggaatgtg
     2881 cttcagaaac tacctgctca gcaaaggaaa gcccgtgtga taaatcacga gcaactgccg
     2941 gtgtgtcatg ccatgcagac gcgttgccag cccattcccg tttattgatt tttcgtccta
     3001 ccagatgcac agaaagatcg ctctcatcga cggtcagact catcatcagg ctggaggtga
     3061 tgaccgtcat ttccctgatg cgagcagcct gttcggcaga taactgcaaa gtacgatcag
     3121 gttctgtggt ttcggtg
//
LOCUS       DAMZII010000262         3087 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZII010000262 DAMZII010000000
VERSION     DAMZII010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.266"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(302..1483)
                     /locus_tag="POA77_004814"
     CDS             complement(302..1483)
                     /locus_tag="POA77_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDI6368124.1"
                     /translation="MKLFGISSRDSGMDAMFYIQASTKQQASFIFDFLATRLYAHCQL
                     IEPLNETIIPSLSRTTLSLQPWSQNSQTRTSEMQMVTHGLTLSGAVMVEHSRQIVDFC
                     DNLGLRLDAPTGKPGWTELINADCEQLNSPRLYPVLIQPGTEGQFQIYVPAPAVIDAD
                     TVIASVIDECFSDVTSYCLAGTPFPMTEKPVIRMEAHLSDAHTRVFELTFISYNLALS
                     ATVYDKNLRQVLMLCEDFGVTLQGRAGASCHAYMRNEYRRLAHVGVYSRSGAENDVRA
                     YSSDKEETLIRQLMTQATAGSALIPARHGKQFNGVLMNANPSSATTGFIYLSPEFPQG
                     QFMMTAEVVKSSCTPRGISYIETVDGYRFIVTAYDTKAMHSLTEFLAVNEDYFAPYRW
                     E"
     gene            complement(1492..1788)
                     /locus_tag="POA77_004815"
     CDS             complement(1492..1788)
                     /locus_tag="POA77_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6368125.1"
                     /translation="MIHSFKDRRLEKFFRNGKSTAGIPAELINAISCRLETLNNVKNE
                     RELLSGSLRYERLRMTANRYSSIRVNSKYRLFFEWNDGVHNVHLSAHDYKSLIH"
     gene            complement(1852..2319)
                     /locus_tag="POA77_004816"
     CDS             complement(1852..2319)
                     /locus_tag="POA77_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368126.1"
                     /translation="MDKYFDRSGMAIDNAKIKCIDSVKGTGEYIYRVTCNKCNGRGER
                     NHFYKSRCIACNATGYSLVTTRTCYTLTALYRIYPEAARKISAAQAAERQRAVQSKTS
                     AFNLWCQNHQELVDAITQQDGENSFLNSLKSTLSRKFPLSDKQLTVAARILGM"
BASE COUNT          774 a          731 c          765 g          817 t
ORIGIN      
        1 gaattaccgg gcatccattg cccggacagt ttattaaaaa aaactgataa tgccccgccg
       61 gagaattacg ccaattcctg tgaaggcatg gtctggctga aaagttactt ttcccggttg
      121 ccctgccttg atcccgtatc gtttataccg gtctgctctt acatcctacc tgggcagcag
      181 ctggctacgg tgatcgggta ttaaaataga accggtctgt tttaccgcag attcaacgcc
      241 ccgcacctag cgtaaaatcg cctttcccgg atgccctcct tggtcgtccg gcacgatggc
      301 gctactccca gcgataaggg gcaaaatagt cctcgttaac agcaagaaat tcggtcaggg
      361 aatgcatcgc cttcgtgtca tatgccgtca caataaaacg gtatccgtcc accgtttcga
      421 tgtagctgat cccccgtggc gtgcaactgg atttcacaac ttccgccgtc atcataaact
      481 gcccttgcgg gaattccggt gacaggtaga taaagccagt ggtggctgat gacgggttgg
      541 cattcatcag cacgccgtta aattgctttc cgtgccgcgc cggaatcaac gcgctccccg
      601 ctgtcgcctg agtcatcaac tggcggatca gggtttcctc tttatctgag ctgtaggcgc
      661 gaacatcatt ttcagccccg gagcgtgagt agacgccgac atgagcaaga cgtcggtact
      721 cgttacgcat ataggcatga caggatgcac cggctcgccc ctgaagagtg acgccaaagt
      781 cttcacataa catcagtacc tggcgcaggt ttttgtcata gaccgtggca gagagcgcca
      841 ggttatagct gatgaatgtc agctcaaaaa cccgggtatg tgcgtcacta aggtgtgcct
      901 ccatgcggat cactggtttc tccgtcattg gaaacggcgt accggccaag cagtacgagg
      961 taacatcgct aaaacactcg tcgatcacac tggcaatgac cgtatcggcg tcaatgacag
     1021 ccggagctgg aacataaatc tgaaactgtc cttcagtgcc cggttggatc aggacggggt
     1081 atagacgcgg ggaattgagt tgttcgcagt cggcattgat cagttctgtc caaccgggtt
     1141 ttccggtggg agcatcaagg cgaagaccca ggttatcgca gaagtcgacg atctggcggg
     1201 agtgctccac catcactgcc ccggagagtg tcagaccgtg cgtaaccatc tgcatctcac
     1261 tcgtccgggt ctgggagttc tgggaccagg gctgtaatga aagcgtcgtc cgacttaagg
     1321 agggaatgat ggtttcattc agaggttcaa taagctggca atgcgcatac agccgggtgg
     1381 cgaggaagtc aaatatgaag cttgcctgtt gttttgtgct ggcctgaata taaaacatcg
     1441 catccatgcc tgaatcccgg gatgaaatac cgaaaagttt cattttttac cttaatggat
     1501 gagcgattta taatcatgcg ccgacaggtg gacgttatgt acgccatcgt tccactcaaa
     1561 gaatagccga tattttgaat taactcgaat ggaagaatac cggtttgcgg tcattcttaa
     1621 gcgttcgtac cgcaaactac cagataacaa ttcgcgttcg tttttaacat tatttaacgt
     1681 ttccagtctg catgaaatcg cattaatcag ttcagcagga atgcctgccg tggattttcc
     1741 attacggaaa aacttttcca atcgcctgtc tttaaatgaa tggatcatat tttcctccgt
     1801 aacgaaatga gtttaaataa ataagaatta aaaaagcctg aatctaattt attacatccc
     1861 gaggattctg gctgcgaccg tcagttgttt atcacttaat ggaaatttgc gtgataacgt
     1921 cgacttcagg ctgttcaaaa aactgttctc gccgtcttgc tgcgtgatgg cgtccaccag
     1981 ttcttggtgg ttctgacacc agaggttgaa cgcggaagtc tttgattgaa cagcacgttg
     2041 acgctctgct gcctgagcgg ctgagatctt gcgagcagct tctgggtata tgcgatacag
     2101 tgcagtcagg gtgtaacagg tacgggtagt gaccaggcta tagccagtgg cattacaggc
     2161 aatacagcgg ctcttataaa agtgattgcg ttcgccacgt ccattgcatt tattgcaagt
     2221 aacacggtaa atatattccc cggtgccttt tactgaatca atacacttaa tcttagcgtt
     2281 atcgattgcc attccgctac ggtcaaagta tttatccatt tttgattcct caggtttggt
     2341 ctgaagtaat aatgacagat tggcctcgct ggctaataac ctgaacgaac gttttttgaa
     2401 aataaatcag gaaggatgat tgagatgatt tatatatgca ggattcaatt ttaatggagg
     2461 gtattttcag aattatctgt ttacaaagaa cgaaacaaat cctaatgagg catttattgc
     2521 atcacaataa aagtgattgc ttttcactca tttgagtagc gatgcttaag tatttatccg
     2581 ttttaaggta taacagcagc gtgatacggc tcagggaatg cctgtccgtc accttaatga
     2641 tgctttactt ccggttgccg aaacagtcca tatggctgac atgtataaca cgcacggtct
     2701 aacgcagttc cggggggtgg cacagcggcg tgatgccgct ttaatctgga aaacgcaggc
     2761 atctactggt gcctcaggat cgcggcactc gcaagctgaa acaccggagc ctgttcgtct
     2821 tccggctgta tcgctcgtcc ccgtctacaa aacccgtctc atccgcaggt atcgctggcg
     2881 cactcccaga cggttagagg ctttcgggaa ctgcctgggt caaagaacac cggcgttcag
     2941 gtagctgttg aaatggacat ctacacgttt ttgttcaagt gattcagttg tggtgagggc
     3001 ataacgggta atgccctccc gtgaagccct gagaaaagtt acttttccag gattaatgca
     3061 gtctggcttt taatataaat tcagggg
//
LOCUS       DAMZII010000263         3065 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZII010000263 DAMZII010000000
VERSION     DAMZII010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.267"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(266..2527)
                     /gene="katE"
                     /locus_tag="POA77_004817"
     CDS             complement(266..2527)
                     /gene="katE"
                     /locus_tag="POA77_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDI6368127.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2710..2973
                     /gene="cedA"
                     /locus_tag="POA77_004818"
     CDS             2710..2973
                     /gene="cedA"
                     /locus_tag="POA77_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDI6368128.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          747 a          755 c          800 g          763 t
ORIGIN      
        1 atgcgtcacg gcataatccg gtagcggcgt aaaaatcgcg gaagggatga aaaaaacagc
       61 gcctgacggc gcagtgtctg gcatgcctgc aatccgggaa accggaccag gaaaaaactt
      121 gcaggccata acagtatcta ccatacactg caatacattg aatttatacg actttgtagg
      181 ccggataagg cgttcacgcc gcatccggca taaacaaagc gcactatgta aatcatttga
      241 ggcggcgcaa ttgcgccgcc tcccatcagg caggaatttt gtcaatctta ggaatgcgtg
      301 accacacgcg gtgtgctgcc atcagcgtta gcagttcatc cataaaacta ccgtcagcgc
      361 tgtcagcttc cacaatccct tcttcaccct ggtcagcgac cttgattgtt gctttaaact
      421 tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt gtaggcttcc atcaggtagt
      481 agttggcatc gccgttgtca gcgatatccg cgatattgcc gcaagggaca atgaccgcat
      541 cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat aggcaacacc gtaccgtcat
      601 ccgcagtcac ttcacccatt cgggagtaga gcagtttggc atgaacgcct ttggccttca
      661 gggccttgag aatggccaga aggtctgccg atctcacttc atcattaagt aaaatcgcta
      721 ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa acttaaggat ggatcctttt
      781 tcagaccgtt gacgtccgga ggtggggtga tattcagctg gtcgtcagtc agttcgatac
      841 cgagattttt cgccaccgcc tgggccagag tgagatcaat atgcgccagc tggtcaacaa
      901 cgcgctcacg aatatacgga cgaacgactt tgcttaactc aaaactgaaa ccatcgacaa
      961 tatggcgctg ctcgaatggc gtctgactta gccagaacag acgcggatgg gaataatatt
     1021 cgccaaacga tggactgcgc tcacgaactt tatttccttc cacgcgttcc tgatatgatt
     1081 caaaaccgcc gcgtttcggt cccggcggtg tttcgcgcgg ccagttatcg ttgatcgagt
     1141 tcggttcgta attcgccggg ttagtgtcga tccccatacg atgcatgcca tcacgctgga
     1201 agttgtggta agggcaggtc ggacggttaa tcggaatctc atggaaattc ggcccaccaa
     1261 gacgactgat ttgtgtatcg gtataggaga acaaacgtcc ctgcaacagc ggatcgttgg
     1321 tgaagtccag accaggaaca atatgacctg gatggaatgc tgcttgttcg ttttcggcaa
     1381 agaagttatc cggattgcga ttgagcacca ttttgccgac acgctgaacg ggcaccagtt
     1441 cttccgggat cagtttggtt ggatcgagaa gatcgaagtc gaacttgaat tcatcttctt
     1501 caggaatcaa ctggaagccc agttcgtatt ccggaaaatc gcctgcttca atggcttccc
     1561 acaactcgcg gcggtggaag tccgggtcac gtccggtgag tttttgtgct tcatcccaaa
     1621 cgagtgaggc tttacctgcc agtggtttcc agtggaaacg tacaaacgtt gccttccctt
     1681 cggcattaat caggcggaag gtgtgaatac cgaagccttc catggtgcgg taactgcggg
     1741 ggatgccgcg atccgacatc gcccacatca cgttgtgcag agtttcaggt tgcagagaaa
     1801 cataatccca gaaagtatcg tgggcacttt gcccttgtgg aattgcccag tgcggttctg
     1861 gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc ctggataaag aagattggcg
     1921 tgttattgcc aacgaggtca aaaataccct cttcagtata gaacttggtg gcaaaaccac
     1981 ggatatcacg cacggtatcg gcagagccag cgccaccctg aacggtagag aacctaacaa
     2041 atactggggt gattttgttc ggatctgaga ggaaatccgc tttggtgata tcgcttaagc
     2101 ttttatatgg ctggaaataa ccgtgagctg ctgatccgcg tgcatgaaca atacgttccg
     2161 ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag aataaaatct tccagcagcg
     2221 ttggaccacg gttaccggca cgcagtgagt tttgatcatc ggcgatgcgc acgccctgat
     2281 tagtggtcag cgcataattt tcactgcctt tgcgtacgtc ttccagagaa ttaagttttt
     2341 cgttacgtgt atcaggggct ttcaggctcc caggggcggt aggttgtgca cctggcggtg
     2401 ttggttcagc cgctggacga tgagatccgt cctcaggtgc cagtgagtcc atccccggtt
     2461 tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg tgggttcttt tcgttatgtt
     2521 gcgacattga actcgtctcc ttaatttatt actgaaaggg ccgctgtttt ggtcaaaaaa
     2581 ccctctaact atagaccatc agccagatcc cgcttcggag actattctgg aaacggtgtt
     2641 atgaaataca cgcaaagtgg gacgtacagg ggctaaatcg gctaaactac agtttttctg
     2701 atttttaata tgcgtttagt gaagccagta atgaagaaac cgctccgcca gcaaaaccgc
     2761 cagattatta gctatgtccc acgcacggaa cccgcgccgc cagaacatgc gataaagatg
     2821 gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg ttgcgttcgt actgatggga
     2881 gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat ccgcccagcg ttgggcaaat
     2941 cagatccgcc aggaagggga agtgactgag taaagattac gtacctcccg gttttgccgg
     3001 gagttacatg acctgaaatt ctgtatttca ttgctacatt cattcaaacc accagtaaca
     3061 aaaag
//
LOCUS       DAMZII010000264         3047 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZII010000264 DAMZII010000000
VERSION     DAMZII010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.268"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(27..1886)
                     /gene="gss"
                     /locus_tag="POA77_004819"
     CDS             complement(27..1886)
                     /gene="gss"
                     /locus_tag="POA77_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDI6368129.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2091..2957
                     /gene="yghU"
                     /locus_tag="POA77_004820"
     CDS             2091..2957
                     /gene="yghU"
                     /locus_tag="POA77_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDI6368130.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTYEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          712 a          821 c          778 g          736 t
ORIGIN      
        1 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
       61 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      121 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      181 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      241 tgatggctga cgaggtcgat attgctacca cagcgaccgg caatcggttt cactgcgtaa
      301 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      361 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      421 ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      481 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      541 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg tccttcccca
      601 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      661 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      721 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      781 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      841 caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc gttgtactcg
      901 taaaccttca gaccacgctc atccatgcag aaatccatac gaccggtgat catatggtga
      961 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1021 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1081 tcgttggtgg ctttaatcag ctcctgctct gcgctctcgg taatggtgta gtaatgatac
     1141 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgtag cggatctttt
     1201 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1261 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cggtatcttc cgtctggatc
     1321 atccagccca gaatggtggt gtcatcaaac gtgtctttaa gggtatagca gccgttttcg
     1381 accaccatct ccagctcgcg agtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1441 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1501 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1561 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1621 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1681 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1741 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1801 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1861 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1921 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     1981 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2041 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2101 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2161 atatcaatcg cccggtttct ggtccgacgt atgaaaaaac gctgcccgtt ggcaaacacc
     2221 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2281 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2341 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2401 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2461 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2521 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2581 tttaccatta cgcgccggtg aaaattgaat acgccatcaa ccgctttacc atggaagcca
     2641 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt gcgggcgatg
     2701 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg ttaggcggcg
     2761 tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2821 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2881 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     2941 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3001 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctg
//
LOCUS       DAMZII010000265         2926 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZII010000265 DAMZII010000000
VERSION     DAMZII010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.269"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..942)
                     /locus_tag="POA77_004821"
     CDS             complement(<1..942)
                     /locus_tag="POA77_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368131.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSQIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYFLKSFGVVF
                     IVLDIYNTYRPENETGFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGF
                     NREDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELD
                     RILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLY
                     DFNIFDTTPYARALLERFHEFESGPKGALTRYIVSLDD"
     gene            1043..1291
                     /locus_tag="POA77_004822"
     CDS             1043..1291
                     /locus_tag="POA77_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6368132.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            1307..2872
                     /locus_tag="POA77_004823"
     CDS             1307..2872
                     /locus_tag="POA77_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDI6368133.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSVMLTVK
                     AVSVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACAKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRIRMTLSHAASHDLEIFHEEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFIVCWDHKLNKYRN
                     EEQTKIWNITTKN"
BASE COUNT          930 a          657 c          559 g          780 t
ORIGIN      
        1 atcatcaagt gacacaatat aacgagttaa tgctcctttg gggccactct caaactcatg
       61 aaaccgttca agtaatgctc ttgcataagg cgttgtgtca aaaatattaa aatcataaag
      121 atagcgtgca gcgtagccat acaacccatg tacccaaagc ccttctctta tcagcactct
      181 gagataatat gcaatactgt caagtatctt taccttccgt tgcatatcaa gcaaaatact
      241 taatgaataa aatgcataat agaacttgtc aatgttgttg agtattctgt ccagctcact
      301 cttatcaagc ttcttctctc tgtaatcctt cttgtattca ttcttacagg cctttatata
      361 ttgtttcata aggctcttcc ttttaccgta cccttttcga atagttccct ggtattttcc
      421 cagctcatac atttttccgt tttcccgaaa atcttctctg ttaaatccca gttcagcaag
      481 atactcccgg agataacgtt taacggcagc ctcatcgctt tctgtaccag agtaatccag
      541 gcagggacaa tatgattttt gcaaaaaatt atagatatga taataaaacc ctgtttcatt
      601 ctccggacga tatgtgttat atatatccag cacaataaaa acaactccaa agcttttcag
      661 aaaatagtta cggatatatt cggactttaa tgttgaatac acagggtaag aattcatcat
      721 gtaataaaaa tattcatacc gggaaaatat aattcgccgt tgttccagac tatacacccc
      781 ttcagcatca ataatttgac tgagctcatt ataacgttga tcccagaatc tttgatgcgc
      841 tccaaaatca gtactcttcc gcttatctct cggactctct ccttccttaa acaactcaaa
      901 aactgtacaa atatcataca aaagatttgt aaacatgcgc atctgctctg atctccggtt
      961 ggtttccgtt ttatcacagg ggacattctg gccttaacgt agcaaattaa tgacatagcc
     1021 ttcaactcac atggagaaca atatgtcaac ccagaccgga catttttata ccccctcacg
     1081 catcctgcgt atggctgaac ttacccgact ccttggtata tcaagatcga ccatctacga
     1141 aaaaatgaat ccggcatcaa aatactacga tgccaccttt ccccggcctg tccggcttgg
     1201 gagttcggct gtaggctggc gggcatctgc cattgatgag tggttaactt tacatacaac
     1261 accaaccaga tccgtaacta aggataaata aggaaaactg tacgccatga caaaattatc
     1321 cccccctgaa cttgacataa ctaaactgcc tgacaggtgc caggcattac ttgatgaaat
     1381 gcatgaagaa acgggaataa gccgtgaaat actgctgtct gttatgctga cggtcaaggc
     1441 tgtctcggtc caggatacgc atgaagttga actttctgga gggcagcgta ccagtcttca
     1501 gatatatatg tgtctttcat cagcatcagg cagtggaaaa acctctgcct gcgcaaaatt
     1561 aatcgcccct gttcatgaaa cagaagaaga actacatcag gcatatatcg acgataaaaa
     1621 aaactatgat cgcatgatgg aaatgtggac aacagataaa aaaatcctgg agcggagata
     1681 taaaaaggaa atggaaagat ccccggaaaa tgctgctgct gcacgagcag cgcttgaaaa
     1741 gtgtatagca aataaacccg tacctcccgt acaacaggtt ctcatcgtga atgacgcgac
     1801 accagaaggg atagctttga aactcagcca gtcaccttct cttctgttgc tgtctgatga
     1861 aggaggaact attctggata agcgtttcga acgcaaatcg gcgctgtaca acacattatg
     1921 gagtgggcaa ccagtcaccg tagaacgggc atccagaccc gggttccggg taaaggattc
     1981 ccgtctcact atgcttatac tgactcaacc ggtaatattt gataagtttt ttactctcac
     2041 tggcgaccag atccggggca atggctttct ggcccgggtg ctattttgcg aacccggagc
     2101 aaataaaata atgacgacag aaccgcatac tgccactcct gttgtaacgc agcaatgtgc
     2161 aacctgtttt catgagaaaa gttttggcag tcagatcaga gactctctga gagcgtcccg
     2221 ggaaaggcgt gcaaaaggtg agcaacgcat ccgcatgaca ttatcacacg cagcatccca
     2281 cgatctggaa atctttcacg aagaaaatat gagtgctgtc aggcagaacc cacgtatgac
     2341 cactttcgaa gacattatcg tcaggaaaag agaacaggct gttcgaatag ccgcacttct
     2401 ggagctggag aacaatccac atggtacagt aataacacga gaaagtatca atagtgctat
     2461 ttatctcgtt gatttttatt ttcagcatct tatatccaaa ctggaatcac ttcgggaaat
     2521 atctcctgca gaaaaactgg ataaatggct cagagaaaat atcatccggg taaaaggata
     2581 cgaatatcaa aaaagccata tactacaata tggtccatat gctctcagga ataaatgtgt
     2641 tcttgatgaa gcacttgata tacttgcaga acagaaaaaa atcgtaattg attattcaat
     2701 cggccaaaaa atcatttata tcggtgacgc gattacacct tgtgaattag caaatgaggc
     2761 aaacatcccg ataatggagc gtggaatgtt tatagtctgt tgggaccata agctgaataa
     2821 gtacagaaat gaagaacaga ctaaaatatg gaacataact acaaaaaatt aaagttcccg
     2881 tcttttatca ttcagttacc aacctgaata tctgaaacaa tgccag
//
LOCUS       DAMZII010000266         2873 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZII010000266 DAMZII010000000
VERSION     DAMZII010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.270"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>2873)
                     /gene="rhsD"
                     /locus_tag="POA77_004824"
     CDS             complement(<1..>2873)
                     /gene="rhsD"
                     /locus_tag="POA77_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDI6368134.1"
                     /translation="DITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRL
                     VSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYE
                     YDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGN
                     RSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGG
                     FDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDGHGWLR
                     EISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVT
                     PDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTST
                     YTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPD
                     LDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRI
                     PTGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRER
                     DLTGWMSLSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREK
                     AQRRSLAEKLQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTV
                     ERLAAQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHH
                     VYQPYRLPGQQHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQI
                     DPMGLLQTWDDARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDME
                     YSGIVCKDTNGKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDE
                     FFSSSDKNLVRSKDNNLEAFYLATPDGRFEALNNKGEYIFIRN"
BASE COUNT          493 a          915 c          745 g          720 t
ORIGIN      
        1 atttctgata aaaatatatt ctcctttatt attaagcgcc tcaaatcgtc catcaggtgt
       61 tgcgagataa aatgcttcaa gattattatc tttacttctt acaagatttt tatcgctact
      121 tgaaaaaaat tcatcaacat aatcgccatg actatctgca ccgtgagtat gataagcagc
      181 aactctatct gtacctgtgg gacattttct tttcagagga tatgactcct ttcttaaatt
      241 atcagtttct gccttagatg caaaatattt tccattagta tctttacaga caataccaga
      301 gtattccata tcattacata gagatctatt ctgcgtttct ttcaaagcat ctaacgcagc
      361 atctgcagta ctatcaaatt tacttggtcc tattatacgt gaaagaacac cacaaactcc
      421 ccccgtacat gctccagatc tggcatcatc ccaagtctgc aataatccca tagggtcaat
      481 ttgttgtagt ggatttaaag gatactgata taaattccat ccccctttca accccatcgg
      541 gtcctgagta atataccgcc cctgcaacgg atcgtagtac cggtgacggt tatagtacag
      601 ccctgattcc tcatcatgct gctgccctgg cagacggtac ggctgataca catgatgcgg
      661 gttctcctca ttaagctggt tgccccattc atcatattcc gcgctccacg ccgtattgcc
      721 gtcttcgctg ataagcgcca gcggcagtcc ccggtggtcg cagtgataaa gatgtatttt
      781 tcgctcaggc aaataaaccg gctctatctg tgccgccagc cgctccaccg tcagcccgca
      841 ctgcgcaagc cactggcggc tttcactgct cacgcagttt gcccggattt ccccctccag
      901 cctgtccagc agccccacca gttcagccgg aaataccacg ccgtgcccgt cctcgctccc
      961 ttcctgctgc agcttctccg ccaggctgcg gcgctgcgct ttctcccgct cgccgttgtc
     1021 tgtttcgatg cggatgagcg gcgcgaagct ccccggctga tataccgtct ggatacgcgt
     1081 ggtgtcggtc tgtaccgtcg tcagccggtc gccgtcccac ccgtaccacg tcatctccgg
     1141 tttacgcgac agcgacatcc accccgtcag gtcacgctcc cgccgccata cccgtttcgc
     1201 cattcgccgt cccagcgggt cgtagaggta gcggctctcg accagtggct cgccatgctg
     1261 tatccgcgtg tagaacacca ggcggtgctg gctgtcgtag tggtagtggt gggtccgctc
     1321 gtcgtccgtc cgtatcacac ccgtcgggat gcggtccgtc ttctccgtca gtctgccgta
     1381 ttcatcatag tggtaaacat agtgcgcatc ctctgcgatg cggttatccg gccacgccgt
     1441 gagcgtgctg tccgggtgta gctccgggtc cggcaggcgg tttcccgccg ggtcggtggc
     1501 atacgggatg cggatgtcca ggtccggtgc gagggtgcgc acgctctcca gcctgcccgt
     1561 ggcactgtag ccgtattccc gcgtctgtcg cggaccgctg atgcgcacca ggtcgccgtt
     1621 gtcattccac ccgtagtcac ggtcatacac caggctgttc aggtgctggc tctgtaactg
     1681 gcctgcgggg gtgtatgtgc tggtcagttc atatgcggca ttactgcctg ccctgctgcc
     1741 gaagctgcgc accgtctcac ggtgcagcct gtcccgcgta tactcgacca gcggcgtccc
     1801 gcccagcttc atgcccgcca ggtaaccgct gccgtaggtc agccactcca ccggcggcag
     1861 gctgtccggc gtgacgcggt ttgccagccc ctgttcgttg tatgcgtgtt tcgtctcatg
     1921 ctgccacagc agttcccccg tctccgggtt ctccaccgtc tgccgttcgc ccgtcaggcg
     1981 gcccttatcg tcgtagccgt agtggacagc gacacggtgg ccttcgctca ggtggctgat
     2041 ttctctcagc cagccgtggc cgtcatactg ccactgctct gccgggtcgc cgttcaccgt
     2101 ccggtgcgtg atgcggtccg acgcatcgta gtgccagagg gtgacaagcc cctcatcttc
     2161 actctgcgtg agttttccgg tcaggtcata atgataacgt tgcgtccgcc cgtcaaagcc
     2221 gccctgctgt accagccggt ccagcgcatc gtaactgaag acgctgtggc tgccgttctc
     2281 gttggtcagg ctgatgacac gtccggcagc atcgtactcc atactgcgcg tcagcccgcc
     2341 ctgcgtggtg ctgaccgcct ttccccacgc atcgtactgt gtctcgctcc ggttgccgtc
     2401 cggggtgata acggcagtca ggtcgcctgc ggcgttgtat tcataccgcg tttcacggcc
     2461 ctgtgcgtct ttcaccgagg ttaaccggcc acggttgtca tagcggcggt aaaggctgat
     2521 gccttcctcg cggtggaccg ccgtcatctg gccgaagcgg tcgtattcat aacgggtctg
     2581 gtagcccgag cagtcggtga acgccagcaa ctgcccgtag cggctccagg tcatctgccg
     2641 ggtgctgccc gtcgcatctg ttgtcgtggc cggtaactca ctgtgcgcgt catcgtagcg
     2701 gtagcgtact gtctccccgc tgcgcgatgt ctccgatacc agcctgcccg gttcatcata
     2761 ttcccggcgg ctctccagcc cgtccgggga caccaccgcc gtcagctggt tcccgtcgtt
     2821 atagtaaaat ttcgtctccc gcccgtccgg tgtggtgatg tccgtgatat cgc
//
LOCUS       DAMZII010000267         2860 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZII010000267 DAMZII010000000
VERSION     DAMZII010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.271"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..330
                     /locus_tag="POA77_004825"
     CDS             112..330
                     /locus_tag="POA77_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6368135.1"
                     /translation="MVKREIKAKTTFIRLPEVIKRTGFGKTWIYELIKTGRFPSQVKT
                     GIRAVAFIESEIDEWIDATIRNSRQNAA"
     gene            412..702
                     /locus_tag="POA77_004826"
     CDS             412..702
                     /locus_tag="POA77_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368136.1"
                     /translation="MKIELITTKQFIEQAEYYFRSYMDGLRRNAPDDFYYFINNKYNM
                     NDIMESIIKKTRYHFYDDTEEGKRNRIYGEVSHSKVKQHLRQLWIIYKCVYR"
     gene            747..1043
                     /locus_tag="POA77_004827"
     CDS             747..1043
                     /locus_tag="POA77_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368137.1"
                     /translation="MNTFKNKTTEIFYVVSLHIYAELFNSKDKTTSNMIITHVMDHEF
                     VCRLIDLAMRNAEKHLLKKAWKKNAAEKLSEVDFKGVKQALAKMHYTVLAESIC"
     gene            1117..1359
                     /locus_tag="POA77_004828"
     CDS             1117..1359
                     /locus_tag="POA77_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6368138.1"
                     /translation="MIDEFHVMYMYKKIQAEAATTDIKTLEQLLKKFRSVVNERREEY
                     YQEIGEKKARKSKMKRAQRLLEKMACDGVSPEDLKK"
     gene            1468..1818
                     /locus_tag="POA77_004829"
     CDS             1468..1818
                     /locus_tag="POA77_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368139.1"
                     /translation="MGFDAKKKHAITIMESKKMWRSNYAPPLLHLAWKAGLKIPPLPF
                     ASFWQITLLMCGWFGPAWGICMWFFTWKDTGMQPGTAFVSSVVAGICFGVLMAAYHWW
                     RKVVNNLPDWDSLG"
     gene            complement(1882..2286)
                     /locus_tag="POA77_004830"
     CDS             complement(1882..2286)
                     /locus_tag="POA77_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDI6368140.1"
                     /translation="MSEALKALNNIRTLRAQARETDLATLEEMLEKLSAVVEDRREEE
                     NSARKEQEERQAKLEAFRQKLLEDGIDPTELLASVGSSQPKAKSTRAPRPAKYKYTDE
                     NGNEQTWTGQGRTPKAIAAAIEAGKTLEDFAI"
     gene            complement(2443..>2860)
                     /locus_tag="POA77_004831"
     CDS             complement(2443..>2860)
                     /locus_tag="POA77_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4756 family protein"
                     /protein_id="HDI6368141.1"
                     /translation="KNHISIGEYRNEYRKLRSDDIPLVKAQKFKSAHTELRRLEKKRE
                     SLIEYFIDELNPISSSKANTSARSTGNLDLFNERVLYRKAISEKSDEEIVALVIKQRT
                     EAAMEFQRSIEQSLEQLSRISSEFEPSSQKSRKMSL"
BASE COUNT          882 a          511 c          626 g          841 t
ORIGIN      
        1 ctgaactcac tggtcctgaa catccctccg gttcagggct ggtgaaccaa cctagttttt
       61 cacaagcgat ctccctacaa ttatctccat cgaataacac aggagataaa tatggttaaa
      121 agagaaataa aagcaaagac aacgtttatc cgtttaccag aagtaataaa acgcaccgga
      181 tttggtaaaa cgtggattta cgaactgatt aagactgggc ggtttccatc gcaggtgaaa
      241 acagggataa gagcggtggc attcattgaa agtgagattg atgaatggat tgacgcgact
      301 attcgaaact cacgccagaa cgctgcataa aaacagaaac aagatctcgt gtcgaacaga
      361 taactgttcg cacagggcat cttattaccc taaacaataa tggagaagaa tatgaagata
      421 gagcttatta cgacaaaaca gtttattgaa caagccgaat attatttcag gagctatatg
      481 gacggattgc gaaggaatgc accagatgat ttttattatt ttatcaacaa taaatacaat
      541 atgaatgaca ttatggaaag tatcattaaa aaaacgagat atcattttta tgatgacaca
      601 gaagaaggta aaaggaatcg gatttatgga gaagtaagcc acagtaaggt aaagcaacac
      661 cttcgtcagc tttggattat ctataagtgt gtataccgct aatactacag catttcattt
      721 ttaatattaa ccaaaaggaa aagccaatga atacatttaa aaataaaact actgaaatct
      781 tttatgttgt gtccttgcat atctacgcag agctttttaa ctcaaaagat aaaacaacca
      841 gtaatatgat tataacgcat gtaatggatc atgagtttgt ctgtaggcta atcgatctgg
      901 caatgaggaa tgctgagaag catcttctga aaaaagcatg gaaaaagaac gctgctgaaa
      961 aactgtctga ggtggatttt aaaggggtta agcaggcatt agccaaaatg cattatacgg
     1021 ttctggcgga atcaatatgt tgatatgttc tgacgtctat cggccctttc atggggccga
     1081 gaacaaatct tatttgtcta tccaaggagc aaaaaaatga ttgatgaatt tcatgtgatg
     1141 tatatgtata aaaaaatcca agcagaagcc gcaaccactg acatcaaaac acttgaacag
     1201 cttttgaaaa agttcaggag cgtggttaac gaacgtcgtg aagaatacta tcaggagatt
     1261 ggtgagaaaa aagcgcgtaa gtcgaaaatg aaaagagcac aaagattgct tgagaaaatg
     1321 gcgtgtgacg gggtttcgcc agaagatcta aagaaatgac tatttgagtt atttttgact
     1381 tacatgcatg gaattgataa tcattagtgt ctattgtggc tttttatcga tagatagatg
     1441 agtaaaggtg gatctggagg gaaccgtatg ggctttgacg ctaaaaagaa acacgctatt
     1501 accatcatgg aaagcaagaa gatgtggcga agtaattatg cccctccttt gctacacctc
     1561 gcatggaaag ctggactcaa gataccaccg ctaccttttg cctcattctg gcaaatcact
     1621 ttgttgatgt gtggatggtt tggtccggca tgggggatct gtatgtggtt ttttacttgg
     1681 aaggatacag ggatgcaacc gggcactgca tttgtctcaa gcgtagtagc aggtatttgt
     1741 tttggtgttc ttatggctgc gtatcattgg tggcgaaagg tcgttaacaa cctgccagac
     1801 tgggatagtc ttggatgaat aatcgaatat tttccgggtc tgttatcttg agaacagtga
     1861 agcccggata tgctgtagcg gttagattgc aaagtcttcc agtgtttttc cagcttcgat
     1921 agcagcagcg attgctttag gagtacggcc ctgacccgtc caagtctgct cattaccgtt
     1981 ttcatctgta tatttgtatt tggctggacg aggagcacga gttgattttg ctttaggctg
     2041 ggatgaacca actgaagcga gtagttctgt aggatcgata ccgtcttcta acaatttctg
     2101 gcggaaggct tcaagtttag cctgacgttc ttcttgttct ttacgggctg aattttcttc
     2161 ctcacggcga tcttcaacaa cagcagagag tttttccagc atctcttcca gagttgccag
     2221 atctgtttca cgagcctgtg cgcgaagtgt acgaatattg ttcagtgcct taagagcttc
     2281 actcatagta tgtcatccgg tgttttaaaa tgggcgatta ataaaaataa tgcttgcggt
     2341 gctgttggaa aaatcaaaaa aacagtcagt aaataatata gtaaacatta tcgtttaagc
     2401 aatctaatac atctcatttc acacgaatag catagggtcg ttttacagag acattttcct
     2461 gctcttctgg cttgatggtt cgaactctga cgagatacga gatagttgct caagactctg
     2521 ttcaattgaa cgctggaact ccatagcggc ttctgtgcgt tgttttatga ctaaggctac
     2581 tatttcttca tcactctttt ctgaaatggc cttacggtat aacacgcgtt cgttaaataa
     2641 atcgagatta ccagttgatc tcgctgatgt gttagctttt gaactgctga tgggatttaa
     2701 ctcatcaata aagtattcaa taagagattc cctcttcttt tcaagtcttc gaagttcggt
     2761 atgtgcagat ttaaatttct gagccttaac caatggaata tcatcagaac gcagtttacg
     2821 atattcattt ctgtattctc ctatcgaaat atgatttttt
//
LOCUS       DAMZII010000268         2854 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZII010000268 DAMZII010000000
VERSION     DAMZII010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.273"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            108..326
                     /gene="ccdA"
                     /locus_tag="POA77_004832"
     CDS             108..326
                     /gene="ccdA"
                     /locus_tag="POA77_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDI6368142.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            328..633
                     /gene="ccdB"
                     /locus_tag="POA77_004833"
     CDS             328..633
                     /gene="ccdB"
                     /locus_tag="POA77_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDI6368143.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            634..1440
                     /locus_tag="POA77_004834"
     CDS             634..1440
                     /locus_tag="POA77_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDI6368144.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVSMLKRIP"
     gene            1620..2264
                     /locus_tag="POA77_004835"
     CDS             1620..2264
                     /locus_tag="POA77_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI6368145.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2351..2659
                     /locus_tag="POA77_004836"
     CDS             2351..2659
                     /locus_tag="POA77_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HDI6368146.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAITATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT          740 a          637 c          723 g          754 t
ORIGIN      
        1 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggcataaga
       61 atatatactg atatgtatac cctaagtatg tcaaaaagag gtgtgctatg aagcagcgta
      121 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatgat gtcaatatct
      181 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
      241 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
      301 ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag
      361 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
      421 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
      481 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
      541 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
      601 aacgccatta acctgatgtt ctggggaata taaatgtcag gctccgttat acacagccag
      661 tctgcagtca tggtaccggc agtgtattct gccgggcagc ctgcatcgct gcctgttgcc
      721 attgattatc cggcagctct ggcacttcgc cagatgtcga tggtccatga tgaactgcca
      781 aaatatctgc tggctccgga agtgagtgcc ctgctccatt acgtcccgga tctgcaccgc
      841 aagatgctgc tggccacact gtggaacacc ggagcacgca ttaatgaagc actggcgctg
      901 acgcgggggg atttttcgct tgcgcctccg tatccgtttg tgcagcttgc gaccctgaaa
      961 caacggaccg aaaaagctgc caggacggcg gggagaatgc ccgccggtca gcagactcac
     1021 cggctggttc cgctctctga ctcctggtac gtcagccagc tgcagacgat ggtggcaaca
     1081 ctgaaaatac ccatggagcg gcgtaaccgt cgcacaggaa ggacagagaa agcgcggatc
     1141 tgggaagtga cggacagaac ggtcaggacc tggattgggg aagcggttgc cgccgctgct
     1201 gctgacggtg tgacgttctc tgtcccggtc acaccacata cgttccgcca ttcctatgcg
     1261 atgcacatgc tgtatgccgg tataccgctg aaggtcctgc agagcctgat ggggcataag
     1321 tccatcagct caacggaggt ctacacgaag gtgtttgcgc tggatgtggc tgcacggcac
     1381 cgggtgcagt ttgcgatgcc tgagtccgat gcggtgtcta tgctcaaaag aattccttaa
     1441 gctctgactt ttttaaatcc ctttcggata tctcatggat atattatcgg tatttcatat
     1501 tctgttgaca tgcaggaccg gtatagagta aaaaaacatc tattgagtac cccttggata
     1561 tccgaaaggt gtcttcgggg ttctttatgg gtattacatg tggattctgg agatacacta
     1621 tgcctgttat tgctaatacg catcccaaag gtggtgtggg taagacaact tcatccgtaa
     1681 acattgttgg tgaaatgaag tctgatacag ttgatctgga tactcatacc gggctttcta
     1741 tcattctggg gctgagacct gaaggaaaag aaatttccgt gaaagtaccc aaaacagtgg
     1801 atgagttaat cgaaattatg actccctaca agaacagcga taagacgtta cttattgact
     1861 gtgggggatt tgactcagat cttacccgta ctgcgattgc ctttgctgac tgcgttattg
     1921 tcccttcaaa agactccctg actgagcgta ttggtttgat gcattttgat ggagtactgg
     1981 acgaaatcag ctctatcatg gggaccgata taactgctca cctgtatctc tgtaaagtta
     2041 atcctaacaa gaagaaattc cctaagcttg atgcaatcct gccatcgttc aaacatctga
     2101 agctgatgaa gagccgcatt tctgctcgtg cagaatttga ggatgtcatt gagactggga
     2161 tgggaatcac tgagtctgtt catgggcgct attcggcagg aggtaaggaa gttattgccc
     2221 tgatcgaaga aatcaatcat ctgattgaaa ataacaaaca gtaaggtgta tctattggat
     2281 atccatatag tatcctttgg gtttttaatg gatatctttt aagtatctaa caaacatcct
     2341 gggttatctc atgagcaaag cgaaatttag tgcacaggct gaaaaactga aaaacctggt
     2401 ggattctgcc agattacaaa cttcttcttc tgccataaca gcaacagccc cgtctcagcc
     2461 aaatgaaggt gcagaggaag tttcaggtcg tcccgtggct gccaggacga aggctaagaa
     2521 tcttaaggcc ataccgctgt cgtattttga agctcatgcg gaacttaaag cgaccagtaa
     2581 aacatctctg gattttagct catatattat tgaggctata agagagaagc ttgaacgtga
     2641 tggtgcaata ggtcaataaa ctatcggata ccttaagggt atctgatgtg tgtcttttaa
     2701 atgcgttatg gggattcttc acgtacctaa agagtcccca ttagacatct ttctgtatat
     2761 ggtgacatga ttcgtcaccg ctttcctttc ctctcagttt caccatttca ttccttttcc
     2821 cttaacctga accatgatgt taaagatgtt ttta
//
LOCUS       DAMZII010000269         2825 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZII010000269 DAMZII010000000
VERSION     DAMZII010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.274"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..523)
                     /locus_tag="POA77_004837"
     CDS             complement(<1..523)
                     /locus_tag="POA77_004837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368147.1"
                     /translation="MNGIWYENTHTRIPNFETTVHQKHKFGYAYETTSHFVHLYGRDM
                     GFNVISVGLTVIEQRSGTLNDWVRRVFGAQNISPLDNEIGHVTRGVWRPSLYYVNDTE
                     TALGIDEFEKRATEQALRVLIEKLDDIFLYVEPSAHGLISYSHKCRELLILACTEVEN
                     QWVSIIGNSNLSRS"
     gene            1087..2037
                     /locus_tag="POA77_004838"
     CDS             1087..2037
                     /locus_tag="POA77_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDI6368148.1"
                     /translation="MELTKPKLFISYSHDSEEHKAWVLKLATRLEQNGVHVILDQWDI
                     TLGSDLTLFMEQGLTSADRVLVICTEPYVAKANMQKSGGVGYEKMIISSQIMRELNSN
                     RVIPVIVNNSLSEPVPTFVGARLRLSFDAVDYENSYVELIHDLHGEKMKPRPAAGPSP
                     FNRKPPPINPVNLSQPDGANQSPTSTGKVTFVPNGNYDSYWVGSGEMLFETKWGVGGR
                     NQAYIYKRQPSLHSLVFAKGITDFNQITDANSEAFFKSEDTESLEVGDIAVIQNINGY
                     YLAVKLESSLYRSRDADDRNEITFSYAIAPAKSTSFAGIV"
     gene            complement(2357..2590)
                     /locus_tag="POA77_004839"
     CDS             complement(2357..2590)
                     /locus_tag="POA77_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HDI6368149.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(2601..2789)
                     /locus_tag="POA77_004840"
     CDS             complement(2601..2789)
                     /locus_tag="POA77_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368150.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYNRSMDVIS"
BASE COUNT          831 a          617 c          546 g          831 t
ORIGIN      
        1 ttgaacgaga taaattcgaa ttaccaatga ttgacaccca ttgattttca acctcagtac
       61 aagcaagaat taatagctct ctacatttat gactgtaaga aatcaaacca tgggcacttg
      121 gttctacata tagaaaaata tcatcgagtt tttctatcaa aactcttaga gcttgctctg
      181 ttgctctttt ttcaaactca tcgataccta atgctgtttc tgtatcattg acataatata
      241 aggatggccg ccacactccc ctagttacat gtccaatttc attatctaaa ggagatatgt
      301 tctgagcccc aaatactctc cgaacccaat cattgagagt tccacttcgt tgctcaataa
      361 cagttaaccc aactgatatt acattaaatc ccatgtcacg cccatacaga tgcacaaaat
      421 gacttgttgt ttcataagcg taaccgaatt tgtgcttttg atgtactgtt gtttcaaaat
      481 taggaatgcg tgtatgagtg ttttcgtacc agattccgtt catcatcacc tctaatgaga
      541 gtcgattata tttcaatgga ttatatggat gatactacac taagttgtac ctatgacaga
      601 tactatttta gccacttcag gcatacagcg cgcgctcgta tccccgccac gcctgcccgc
      661 tttatgtagt ggttttcatg cacctgcatg agataagcaa aagcccgcca attctggcgg
      721 gcctcagcaa agatgatcct caaacgatca tgcgatttca tgcggcatag tcatgcacta
      781 ctaagggaag tgcaaatccg tatctgtatg accgcttgaa aaacgaaaca tacggtttta
      841 caaagatgaa agtccacagt gagcgaaggg caaacgctgg ctttgaattt acctaaaccg
      901 actgctggac actacctaca agagaatggt tccgtttgaa atggttactt aaactagaat
      961 cagatgcatg ttgtaaataa tttaaattaa agactcatta taatttaata gtttcgagat
     1021 aaatcacaga tttcacttag tattcatcgt atgaatcgta tggcatatat aaaacaatgg
     1081 gattttatgg aactaactaa acctaaactt tttatttctt attctcatga tagtgaagag
     1141 cataaagcat gggtgttaaa gcttgcgacc cggcttgaac aaaatggagt tcacgtaatc
     1201 ttagaccaat gggatattac attgggaagt gatcttactc ttttcatgga gcaagggctt
     1261 acaagcgctg accgagtgct tgtcatttgt acagaaccat atgtagctaa agccaatatg
     1321 caaaaatcag ggggggtagg ttatgagaaa atgatcattt ccagtcagat aatgcgagaa
     1381 ttgaatagta atcgcgttat ccctgtaatt gttaataact cattatctga gccagttcca
     1441 accttcgtag gtgcgcgcct tcggctttct ttcgatgctg tggattatga gaatagttat
     1501 gtagagctga tccatgattt gcacggtgag aaaatgaaac cacgcccagc ggctggacct
     1561 agcccattta accgaaaacc gcctccgatc aaccctgtca atttatctca accagatggg
     1621 gctaaccaat cccccacaag cacaggcaaa gttacatttg tgccaaatgg aaattatgac
     1681 tcttattggg ttggctcagg tgaaatgctt tttgagacaa agtggggagt tgggggacgt
     1741 aatcaagcct acatttacaa gcgtcagcca agtctccatt ccttagtatt tgctaaaggc
     1801 attaccgatt tcaatcaaat cacagatgcc aattctgaag cattcttcaa gagtgaagat
     1861 acagaatcgc ttgaagttgg cgatattgca gttattcaaa atattaatgg ttattatttg
     1921 gccgttaaac ttgaaagtag tctttatcgt agtcgcgatg ccgatgatcg taatgaaata
     1981 acattctcat atgccatagc cccagcaaaa agcacctctt tcgctggtat agtttaaaat
     2041 gttgagagtg gaaacttcca taaattcaat gtatttttaa gaaatagata catttttgat
     2101 aagcaagcgg ccctttcttg ggccgttaac gattttccgg caggatggat ttcactacct
     2161 tctcctgaag gcaaattagt atatatgata tttaagtccg cttatggctg tgagttcaac
     2221 ggatcgatgc aaccctatcc atcggttgaa ctcacagcac aaagcggacg atcaattatc
     2281 aaaatggtcg cccaccttac gcctcgtttc ctaaactagc ccccatcaga atgaatcctc
     2341 ctgggggcaa cgtttcttaa tgcagccagc tgtcgtcttc ccacaccttc tgcataattt
     2401 tcatcacttg ttttctttct tcgtccagtt gcaatccggt aagttccaca ccgttagagc
     2461 tacctttgcg gatacgaatt accgttttgg gatacagggg gcgcagattg cggtaaagct
     2521 cggattcaag ggcgtccagg gtagactggc taatcttctg ctctttatcg atcattattt
     2581 caatgcgcat aaaagtcacc tcagctgatg acatccattg agcggttgta ttcgtggctt
     2641 ctgatttttg ccatgagttc atcagtcaat tcagaaaccc actgcagagc cagccccttc
     2701 tcttcatcac tacactcact agccgctaca agcttaagaa aaaaatcaat gcgctggagc
     2761 ttcaaagact ccaaaaaata gtcctgcatc tttcctccta tgacaccaca cgcaatgctg
     2821 tatgt
//
LOCUS       DAMZII010000270         2780 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZII010000270 DAMZII010000000
VERSION     DAMZII010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.275"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            90..2369
                     /gene="ydeP"
                     /locus_tag="POA77_004841"
     CDS             90..2369
                     /gene="ydeP"
                     /locus_tag="POA77_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDI6368151.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYTAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITCKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2617..>2780
                     /gene="safA"
                     /locus_tag="POA77_004842"
     CDS             2617..>2780
                     /gene="safA"
                     /locus_tag="POA77_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDI6368152.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDL"
BASE COUNT          738 a          644 c          678 g          720 t
ORIGIN      
        1 atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct
       61 aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg
      121 ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata
      181 tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg
      241 gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      301 aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata
      361 cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga cgtctcactc
      421 agcctttgaa atatgatgct gtcagcgact gttacaagcc attaagctgg caacaagctt
      481 tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      541 cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg
      601 ggagtaataa ctttcccgac tgctccaaca tgtgccatga accgaccagt gtgggtttgg
      661 cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt
      721 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc
      781 gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc
      841 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
      901 tggccagtgc ctactataac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga
      961 tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgcttg
     1021 atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca
     1081 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1141 tggctgacgc atataccgct gccgaacgca ccattatctg ttacggaatg gggatcactc
     1201 agcacgaaca tggtacccag aatgtacagc aactggtcaa tttgctgttg atgaaaggta
     1261 acattggcaa gcctggtgcg ggtatctgcc cactacgagg tcactctaat gtacagggcg
     1321 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc cttggtgagc
     1381 gctatggctt caccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa
     1441 tatgtacggg gcaggctcga gcattgatct gcatgggggg caattttgcg ctggcaatgc
     1501 cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca
     1561 ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg
     1621 ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa
     1681 tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag
     1741 agtgtgctgt ggtcgcggga atcgcccagg caacactacc ccagagcgtg gtagcctggg
     1801 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1861 tcgccgacta taaccagcgc atccgtcatc ctggaggttt tcacctgata aatgcagctg
     1921 ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattacctgc aaagggctgt
     1981 tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg
     2041 atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac
     2101 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2161 gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat
     2221 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga
     2281 atcacatgct aactcttgat aaccacgatc cattaagtgg cattcctggt tataaaagta
     2341 ttccggttga attagaacca tcaaattaat tcgcctcctc atttcttctg ctgtcatccg
     2401 cacagcagaa gaatttctca ttgactatta tcgcgcaatt tgctcacatt gattaaatta
     2461 aaccacacac tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact
     2521 gaagcaccag tcctatttcc tcttttctcc agcctgttat attaagcata ctgattaacg
     2581 atttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac
     2641 aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt tgtcttatta
     2701 ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat ttcagaaatg
     2761 ggtcgcattg caagagatct
//
LOCUS       DAMZII010000271         2771 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZII010000271 DAMZII010000000
VERSION     DAMZII010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.276"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            95..598
                     /locus_tag="POA77_004843"
     CDS             95..598
                     /locus_tag="POA77_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6368153.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(644..1060)
                     /gene="rraB"
                     /locus_tag="POA77_004844"
     CDS             complement(644..1060)
                     /gene="rraB"
                     /locus_tag="POA77_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDI6368154.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1222..2226
                     /gene="argF"
                     /locus_tag="POA77_004845"
     CDS             1222..2226
                     /gene="argF"
                     /locus_tag="POA77_004845"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6368155.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2327..2557)
                     /locus_tag="POA77_004846"
     CDS             complement(2327..2557)
                     /locus_tag="POA77_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368156.1"
                     /translation="MKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKTNEIVKN
                     NPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
     gene            complement(2544..>2771)
                     /locus_tag="POA77_004847"
     CDS             complement(2544..>2771)
                     /locus_tag="POA77_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368157.1"
                     /translation="LFFNLDKEHYKNSRKLQELIWDKLVAYVNDFNLSNQEKSRLILR
                     LFDDVKLLFNEVPVSILVNDIFFEGFFHEAT"
BASE COUNT          753 a          631 c          623 g          764 t
ORIGIN      
        1 caaaaacttt attactttat aagtcgcttg cgcacgtaag aagagagtgg tattaaacaa
       61 atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc cgcaatcacc
      121 tgtaatgcgt cgcctgacgc agcaggataa tcccgccatc gcccgcgtca ttcgtcaggt
      181 atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc cgaacctgga
      241 cgagttgtat caagtgtata gtcagccggg tcatgcatat tgggtggttg agtacgatgg
      301 tgaagtggtc ggcggcggtg ggattgcgcc attaaccggg agtgagtcgg acatttgcga
      361 actgcagaag atgtattttc ttccggctat tcgcggtaaa gggctggcga aaaaactggc
      421 cttaatggcg atggagcagg cacgagagat gggcttcaaa cgctgctatc tggaaacgac
      481 cgcttttttg aaagaagcga ttggtttgta tgaacattta ggttttcaac atatcgacta
      541 tgcacttggc tgcacgggcc atgtagattg tgaagtgcgg atgctacgcg aactctaaaa
      601 aaatgcccgc tagtgtgtgc tatcgggcat tttaatcaat taattagtgg cgaacaccgt
      661 catcgtcttc atcgacaaaa tcttcatcgt cgccgtcttc gccattggga tcttcaaagt
      721 aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc agttgttcaa
      781 cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg cagcaaatca
      841 cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa ccgagtttaa
      901 acgcttcaac tgctgctttt tccagggttt ccagatcgtc tgcggaaaga tggtgttcga
      961 tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata atcaaacgtg
     1021 tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc tcttaatgtc
     1081 ctgccgatac tcttattgtc acacaccact gacattgcct ccacctttgt gacaaagatt
     1141 tatgctttag acttgcaaat gaataatcat ccatataaat tgaattttaa ttcattaagg
     1201 cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct gaaattactc
     1261 gatttcacgc cagctgaact caacagcctg ctgcagttag ccgcgaagct gaaagccgat
     1321 aagaaaagcg gcaaagaaga agccaaactc actggtaaaa acatcgcgct catcttcgaa
     1381 aaagactcga cccgtacccg atgctctttc gaagttgccg catatgacca gggtgctcgc
     1441 gttacttatc tcggcccaag cggcagtcag attggtcata aagagtcgat taaagacact
     1501 gcccgcgtgc tgggtcgcat gtatgacggt attcagtatc gcggctatgg tcaggagatt
     1561 gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac caatgagttt
     1621 caccccacgc agctgctggc ggatcttctc accatgcaag agcatttgcc cggtaaagcg
     1681 ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg caattcgatg
     1741 ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg
     1801 ccggaagctg cgctggttac ggaatgccgc gccctggcac agcaaaatgg cgggaatatt
     1861 acgctgacgg aagatgtagc caaaggagtt gaaggtgctg actttatcta taccgatgta
     1921 tgggtgtcga tgggggaagc aaaagagaaa tgggcagaac ggattgcatt actgcgtgat
     1981 tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa attcctccac
     2041 tgcctgcccg cgtttcatga cgaccagacg acgcttggta agaaaatggc ggaagagttt
     2101 ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc cagcattgtt
     2161 tttgatcagg cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc gacgctcagt
     2221 aaataattga tatcgctata aagatgccgg gaaatacaaa aacatcccgg catcacatta
     2281 ttttatgcag taatagatca attattccat ccatattttt ctattgctat acgtaaatct
     2341 tccggaggat tttcatcatt cttgttctaa tttttaattc attaaccatc ttttcttcaa
     2401 aaatcttaac gacataaccc ggattgtttt ttacaatttc atttgttttt tcttcattac
     2461 cataaacata tcctaactca ttaagataca gctgttcacc ctcataatct ttaagtaatt
     2521 gatagaaata ccacttagca aaatcaggtt gcttcatgaa aaaatccttc aaaaaaaata
     2581 tcatttacta aaatgctgac aggcacttca ttaaatagca acttaacatc atcaaataac
     2641 cttagtatta agcgtgattt ttcttgattg cttaagttaa aatcattgac atatgcaacc
     2701 aacttatccc aaatcaattc ctgtaattta cggctatttt tatagtgttc tttatcgaga
     2761 ttgaaaaata a
//
LOCUS       DAMZII010000272         2758 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZII010000272 DAMZII010000000
VERSION     DAMZII010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.277"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            609..929
                     /locus_tag="POA77_004848"
     CDS             609..929
                     /locus_tag="POA77_004848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368158.1"
                     /translation="MQQFRHWQNRLNTIDPARIKLYSETVRVWNQSRGSAGARALADM
                     LIQNDIPMSSYRARRLMKHLNLRSAQPGKYQYKNARQEHTSLPSLLECRFSVLEAEQV
                     WILK"
     gene            complement(1603..2532)
                     /locus_tag="POA77_004849"
     CDS             complement(1603..2532)
                     /locus_tag="POA77_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6368159.1"
                     /translation="MNSIFTEENLLAFTMAARYRSFSKAAKELGLTTSAISYTIKRME
                     MGLDVVLFTRTTRSIELTEPGHYLFCKATDLLNDYHSIIRRVDAIAQGIKSRVRICIN
                     QLLYTPQHTARLLQVLREHFPTCQIAITTEVYNGVWDSLINNQADIAIGAPDTLLDGG
                     GIDYIEIGMIRWLFAISPAHPLAFIPEPISESQLRLYPNIMVEDTANTINKKVGWLLH
                     GQESILVPDFNTKLQCQILGEGIGFLPDYMVREAMEKSLLVTRQIHNPRQDSRMLLAT
                     RHSASGQVTQWIKREFSLQGILTGIYQDLMHRA"
BASE COUNT          756 a          643 c          620 g          739 t
ORIGIN      
        1 gggatgccag cctggcgact ataactccga aaagaccgat tattgtattg cgtgcatccg
       61 gcatgtttac aatgtacgct ggtatctaac ggagtctgac acagatgcat gcaggtgtct
      121 attttaacgt aattatccgg aaagaacagt atattttaat gcgggagttg catgagcggt
      181 atacgggaga aactatcaga ccatgggagc tacagaggtg cttaagtctc gtaacattat
      241 aatctgctca cctgactggt gctattctca cctcaacacc aatgcacaaa tagcctggct
      301 tatcgttaat aaaagttact cataccggga atccagtgaa gctatgaatg tcggttccta
      361 tacactgggg gcatgggtac gtcagctcag gcgggaacgt caggatacca cgtcttctgc
      421 atcaccactc acatcagagt agcaacgtat tcatgagctg gaaaagcagg ttcgtcgcct
      481 ggaggaacaa aaaacgatat taaaaagcta cagtgctctt gatatcctcc ccactgaacg
      541 gtttacgata gttaccagac tgagcggcga cacacggttg tcagtctttg cgctgctctg
      601 gatgctcatt gcagcagttc cggcactggc agaatcggct caatacgatt gatccggcgc
      661 gaatcaaatt gtatagcgaa acagtccggg tctggaacca gagtcggggt tccgcaggtg
      721 cgcgtgctct ggcagatatg ctgattcaaa atgatatccc gatgagcagt tatcgtgcca
      781 ggaggctgat gaagcatctg aacctgagaa gtgctcagcc cggaaaatat cagtacaaaa
      841 atgcccgcca ggagcatacc agcctgccga gtctgcttga gtgtcggttt tctgtgctgg
      901 aggccgagca ggtatggatt cttaaataaa cctcgagtct gaatcggtaa ccagctgtta
      961 tattctgaat tattacaata gcgtcaggcc tcataattac actgtgggtt gacgccggaa
     1021 gaatcagaga acagataccg ttcttactgt aaaacgtggc taatattaat tgagcactgc
     1081 agtgttcaga gcacaaagaa gaaaaaagat gtgtgcgaat gcctgtaatg tgttaaaaga
     1141 atgttccgcg tttccggtac gcttttcgta actaacaatg cagggtgacg taacgctggt
     1201 tgtgccggaa acgcgtccct ccccctatct ttcctgtttg gatttgcccc tatatttctg
     1261 gattatccga caaaaccggc gtgaagtatt actctcgtcg cccagcacca taaggataac
     1321 gcccataaaa tcatgaaact tttcttccag attggataca atcaggccga aacggtgatg
     1381 aatattatcc aaatctgatg gttcgtagcg ttttcagccc ccagattctg agttgcctgt
     1441 agggatgatt tcaattcccg atttgtctat cgcatagaga aaaagaattt catttctgtt
     1501 caccagaacc catgtaaccc actccaattc gttatttatc tcactgattg cccttcattt
     1561 atctcattga tgcaccgaaa ggtaaaactg ccatccggtg catcacgctc gatgcatcaa
     1621 atcctgataa atccccgtca gtattccttg taagctaaat tctcttttta tccactgagt
     1681 gacttgaccg cttgccgaat gcctggttgc caacagcatt cgtgaatcct gacgtgggtt
     1741 gtggatctgc cgtgtcacca gcaatgactt ttccatcgct tcacgcacca tatagtctgg
     1801 cagaaaaccg attccctccc ccaagatctg gcactgaagc ttagtattaa agtcgggaac
     1861 cagaatcgac tcttgcccgt gtaacagcca gccgactttt ttgtttatag tgtttgcagt
     1921 gtcttccacc atgatgttcg ggtacagacg taactgactc tcagaaatgg gctcaggaat
     1981 aaacgccagc ggatgagccg gagaaatcgc gaacaaccaa cgaatcatgc cgatttcaat
     2041 gtaatcaatt ccaccaccgt ccagtaacgt atcgggcgcg ccaatggcga tatcagcctg
     2101 gttattaatg agtgaatccc agacgccatt atacacctct gtagtaatag ctatctggca
     2161 ggttggaaag tgttccctga gcacttgcag aagccgagct gtatgctgag gggtatacaa
     2221 cagttgatta atacaaatgc gcacccggga tttaattccc tgagcgatgg catcaactct
     2281 acgtatgatg gagtggtaat cgttgagcag gtcagttgct ttgcaaaaaa ggtagtgccc
     2341 gggctcggtc agctcaatgc tacgggtagt tcgggtaaaa agcaccacgt caagccccat
     2401 ttccatacgc ttgatagtgt aactaattgc cgatgtggtt aagcccagtt ccttcgccgc
     2461 cttgctgaaa cttctatagc gggcagccat agtaaatgcc agtaagtttt cttccgtaaa
     2521 tattgaattc atctttcttt cctctggcga caatacacat acacaacatg atgattgttg
     2581 aatgtatttg taattcttat aaccgacaat cagttttgaa aaaactaaca ccgtatttac
     2641 atttaatttg aaggaaatca cacatctgaa ttttaattac aggcagctat ccgtgctgca
     2701 gaccaccttt atttatatga ctgcggatta atggctaaaa tatccccagc tataatag
//
LOCUS       DAMZII010000273         2757 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZII010000273 DAMZII010000000
VERSION     DAMZII010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.278"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..700)
                     /locus_tag="POA77_004850"
     CDS             complement(<1..700)
                     /locus_tag="POA77_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication-associated protein G2P"
                     /protein_id="HDI6368160.1"
                     /translation="MIDWFTGILPCTHRPLPAGSVVSVDADGAVEWETVKRLTVRGSH
                     ESTMKVRSVGSDGEGRATHLYIDGNPSKFLQGHSVIGSDDLQGLVLTAYARILALLHI
                     PHDLPSYRQVMKGQFKISRIDINYMYSLSTLENVRAWLYAAEFKAKTRHGRACSKGGT
                     VYLGKNSRRWSLKFYSKYDEHTSGKKGHQMADEFVKAGLLDWSKDKLRIELTLRTTEL
                     IDLNLTLGNSWNIET"
     gene            872..1318
                     /locus_tag="POA77_004851"
     CDS             872..1318
                     /locus_tag="POA77_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6368161.1"
                     /translation="MGNNEHEVMADKQRAERIKKVILEHSTYEELAEKTGISVSTLVR
                     IASGKTEPKFSDIIQIAKITGADLNTLAYGYALDVKEEATERKLITSADGYTDEETTN
                     AHNFIVWNIRTLEKQDILALARQVSALSSYTYSTKMFMKKAVSGEE"
     gene            1418..1837
                     /locus_tag="POA77_004852"
     CDS             1418..1837
                     /locus_tag="POA77_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368162.1"
                     /translation="MSRNNETSGVELVVVGVFAFCLAVVAWLMKTFDVEWQTALETAP
                     GLVVWLLAVGAGIFFGIKMETGLVRWGAPLAIALLIPVFKPILKEAAGVREMGGLVFD
                     DMVSWYGTGWGMSLMFFGILIIGYGLLYWWHRRNSYY"
     gene            1924..>2757
                     /locus_tag="POA77_004853"
     CDS             1924..>2757
                     /locus_tag="POA77_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368163.1"
                     /translation="MAIFELFSKRQKKLRGEVADVYQYTNIPNAFRVQVVHIVRDTIG
                     EDSDYETEASKLYRLVHKTLCKEYGVFSLKQYPESNFGAIFDYFLAERDHEKCLDIIE
                     ICFKLIDGYIRSNEWKFRGLVKQSPDDAIEELNIRFKEAGLGYQFESGELVKVDSQFI
                     HSDVVKPTLQILGKNKSYNGANDEFLSAHEHYRHQRYKECLNDCLKSFESLMKAIHKK
                     HSWQFNDTDTAKKLINSCLANNLVPEYLQNQFSSIRILLESGIPTIRNKEGGHGQGAE
                     IT"
BASE COUNT          870 a          508 c          604 g          775 t
ORIGIN      
        1 gcgtctcaat attccagcta ttaccaagcg taagattcaa atcaattaat tcagttgttc
       61 ttaatgttaa ttcaatgcgt aatttatctt ttgaccagtc cagtaaacca gctttaacga
      121 attcgtctgc catctggtgc ccttttttgc cagatgtatg ctcatcatat tttgaataaa
      181 atttcaggct ccaacgacgg gagtttttcc ctaagtaaac agtgccacct ttactacagg
      241 cgcgaccgtg gcgagtttta gctttaaatt ctgcggcata taaccatgcg cggacatttt
      301 ctaatgttga caatgaatac atgtaattga tatcgatgcg cgagatctta aattgccctt
      361 tcatgacctg ccgataggat ggtagatcat gaggaatatg cagtaatgcc aatattctgg
      421 cgtaagctgt taacaccaac ccttgcaaat cgtcggaacc gataacagag tgaccttgca
      481 aaaactttga tggattaccg tcaatgtaga gatgtgttgc ccgaccttca ccatcagatc
      541 caacagacct tactttcatt gtggactcat gcgaaccgcg aacggtcaac cgttttactg
      601 tttcccactc cactgcaccg tcagcatcga cgctgacgac actaccagcc ggtaacggtc
      661 ggtgtgtgca aggcaggatt ccggtaaacc agtcgatcat aagtagtcac acccatcaaa
      721 atatgtgtca atagtcattt gtgagcagat tgaatggtca caatgtaata tgctcagatc
      781 tgacagtcaa tacatttgca atgtttaaat tgtcagaaat gagcatcaaa tctaactatt
      841 gaaaaaccac tgttaagatg gagccaagta gatgggcaac aatgagcatg aagttatggc
      901 ggacaaacag agagcagaac gtatcaaaaa ggtaattctg gaacactcga cttatgaaga
      961 actggccgag aagactggca ttagcgtgag tacgctggtc aggatagcgt ccggcaaaac
     1021 tgaacctaaa ttcagtgaca tcattcagat agctaaaatt acgggtgcag acttaaacac
     1081 cttagcttat ggctatgctc ttgatgttaa agaagaagct accgagcgga agctaatcac
     1141 ttctgcggat ggatatactg atgaagaaac cactaacgca cataatttca tcgtgtggaa
     1201 catcaggaca ctggaaaaac aagatattct ggcactcgca aggcaagttt ctgccttaag
     1261 ttcatacact tacagtacta aaatgtttat gaaaaaagcg gtgtctggag aagagtaaaa
     1321 ggattaacag tctcaggaat gagacaaacg tgcagtatta ccaacactgc acgttcttta
     1381 ttgtgcgaat tttgaacagt gaaaaaggat caaaaggatg agtagaaata acgaaacaag
     1441 cggcgttgaa ctggttgtcg tcggcgtatt tgctttctgt ctggcagttg ttgcctggct
     1501 aatgaaaact ttcgatgttg aatggcaaac agcactggaa actgcgcctg gcctggtagt
     1561 ctggttgctt gctgtcggag cggggatctt tttcgggatc aaaatggaaa caggtcttgt
     1621 tcgctggggt gctccactgg cgatcgccct tctgattccg gtattcaaac caattcttaa
     1681 ggaagctgcc ggagtacgtg aaatgggtgg gttggttttc gatgatatgg tttcgtggta
     1741 cggaactggc tggggaatgt cgttgatgtt tttcggaatt cttatcattg gatacggact
     1801 tctttattgg tggcacagaa gaaactcata ttactgataa ctccaccgtg gaaaacgact
     1861 gtaaaagcgg tgtgattttc atcaactcag acatattaat ggcatatctt tgaggattat
     1921 aaattggcta tattcgaatt attttcaaaa agacaaaaaa aattaagagg tgaagtagct
     1981 gatgtttacc aatacacgaa tataccaaat gcatttcgtg ttcaggttgt tcatattgta
     2041 agagatacta tcggtgaaga tagtgactat gaaacggaag caagtaagct ttacagactc
     2101 gttcataaaa cgctgtgtaa ggaatatggt gtattctctc tcaaacagta tcctgaatcg
     2161 aatttcgggg caatttttga ctatttccta gctgagagag atcatgaaaa atgcttggat
     2221 atcattgaga tatgtttcaa actgatagac gggtatataa gatcaaatga gtggaaattc
     2281 agagggttag taaagcaaag tcctgatgat gctattgaag aacttaatat caggtttaaa
     2341 gaagcaggtc ttggatatca atttgagtca ggagagttgg ttaaagttga ctcacaattc
     2401 attcattctg atgttgtaaa gcctacgctt cagattcttg gtaagaacaa gagttataac
     2461 ggagcaaacg atgagttttt atccgcccat gagcattata gacatcagcg atataaagaa
     2521 tgtttaaatg attgcctcaa atcttttgaa agtctaatga aagccataca taaaaaacat
     2581 tcttggcaat ttaatgatac tgacacagct aaaaaactaa ttaacagttg tttggcaaat
     2641 aatttagtac ctgaatacct ccagaatcaa ttttcttcaa taagaattct acttgagagt
     2701 ggtattccaa ccattcgaaa taaagaaggt ggacatggac aaggggcaga aataaca
//
LOCUS       DAMZII010000274         2747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZII010000274 DAMZII010000000
VERSION     DAMZII010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.279"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(175..1564)
                     /locus_tag="POA77_004854"
                     /pseudo
     CDS             complement(175..1564)
                     /locus_tag="POA77_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1876..2025)
                     /locus_tag="POA77_004855"
                     /pseudo
     CDS             complement(1876..2025)
                     /locus_tag="POA77_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2203..2679)
                     /locus_tag="POA77_004856"
                     /pseudo
     CDS             complement(2203..2679)
                     /locus_tag="POA77_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
BASE COUNT          675 a          680 c          652 g          740 t
ORIGIN      
        1 accgcgaagc ggttcggggt tctgtgacac ccgaatttta cctcggtttc ctttgtatat
       61 aatatcattg aagttatttg catttctcag aaatcttctc tggctttgat tgcaatagcc
      121 gaaagatagc gcacacatcg ccgttggtca gttagtacga ggtttccgcc aagttcacct
      181 tttaccaaag atggcctcag agcttccctg aagctttatc atggcttcaa gtaggtcatc
      241 ttcgttcagt gagcgctgag atttgttgat gctaggggcg tgccgtctgc gggaaggctc
      301 atcaccagaa caaggtaacg attgtgagcg attattgtcc cgttttctct ggacctgacg
      361 ggcaacatcc aacacatggc caagtctctt gttatctaca atggcgacgg gatcaacctc
      421 agaaagccgg tcataggtgg agtagggcag cagcacgcca tttagtcgca gctccttatg
      481 tccgtcagga taatgccaga catcaatata cttgccgatg gctcttcgac tgtactcgtt
      541 gtcctcaatc agatagagca ttttatcgta ttgtacagtc agattttttg atacccggcg
      601 aggctctcgc caggtaaatt ctgcatcaag gtcatcgtct aatgccagcg gccggtgcgc
      661 gttaaaatca tgtaagggtt ctttggcaaa gcgccggtta taatccttct gcaaatgcat
      721 tggccgcttc tattgtacag atccccttaa gcctgagctc cttaacgagt cggtcctgaa
      781 gtgtcaaatg agcacgttca acgcggcctt ttgcctggct ggtttcagca cacaaaggag
      841 taatattaag gcagcgcatg gccctggcaa actgggtttc gccggggccg gtggtggcat
      901 gcttattatt gactctgaat acgctggctt tatcgctgta gaggatcatc ggtttaccgt
      961 atttctcgat atagccacgt gtagcttcaa aataggtaaa tgtggactca gatttcacaa
     1021 acagcaggtg catcaggcga ctggtggcat cgtcaacata gaccagggca gaacagggtc
     1081 gaccccggtt ttcaaaccag tggtggtcgc agccatcaat ttgtatcagc tcgccagtac
     1141 aaggacgccg gtaacgaggc tgatgaattt tcggtgcgcg ttgtctgcgg ggaatccaga
     1201 gtccggcctt tatcatgaga cgtcggatgg tttcctttcc aaggaccaga ccgtgaactt
     1261 cctccagctt ttcccgcgct agcgtggggc caaaatcacg gtagtgctcc cggattatac
     1321 tgagtgcctg atctgtcagt gacgctggca acagacggtt accagtgcgg ccacgactct
     1381 ggttgttcat gccaagcggc ccatactggc gataacgttt gagcagacgg ctgcaatgac
     1441 gtggcgtgat gcccagcatt tcggcagcct gtccaggacg gatattgcga tcgatgacat
     1501 cctgaaggat tttgaggcga ttgatttctt tcacggtgaa cattccggcg tcatttatag
     1561 ccatagaaaa ctcctcatca ggaaaaagtg agtcgttgtc tagacatagg gatctgagaa
     1621 actatagcct ggacattcct aaaatgagcc acaaatagac attagcattg agcctctaca
     1681 atgtttgtgc gcacaatcta tcttatgtta aataacattg gatattaact tacttcatga
     1741 agcttactgc ttctatttcc acttcttttt cttattaatc gctcccctaa tccacgcatt
     1801 catctgctct acaggctcta aaatattttt tctgagtact gagcacaaac tgctccataa
     1861 aaaacagacc acaagtttgg gcatgatact gatgtagtca cgttttattg ttttcatgaa
     1921 gctctctgct attccattac tcaccggact ccgcaccgcc gtgttcttcg atttaagtcc
     1981 caacatccga gcgaactggc gtgtttcatt aacccggtag tcagagtcag cggtcgaaag
     2041 acctcccagg tagctactct gcatacccac tataatgcaa ctattctcgt gacgtgtgtt
     2101 tcagctctcc cccggctatg atacgagatg gtatggagag cccttctggg gccactgcct
     2161 gcaccagatg caacaggaag cctgatatga atgcaatttc tgtcaggaca ggcggataac
     2221 tttctgtggc catggatcag ggaatactcc acccgacatc caccactgaa tttcttcttc
     2281 agtcaacaag caaagggata aggcatgtgt aaaatcatta acttcagtgg cattgccaat
     2341 gatagtcaac tgacagcagc gatcgccaaa tcgcccagag gctgtgtcga tgtgtcgctg
     2401 tagcgctgaa cgttcttcac gagttaaatt attgtcagta tgttccagta ctccagcttt
     2461 ccagtagcca atgagcgcca gactgatgct tcctgctgac tgattccaga gcagggcaag
     2521 atcatctctc ccggggagcc aaaaaaaacc tttactgcga taaacgccca tgcccataaa
     2581 gcggtgacag gtatcccata atctttgcgg atggaatggc ctgtcgtccc tgatgacgcg
     2641 ccatataagt tcctcattgt tgcacggagt atccatgtga agtggtttac tgaatttggc
     2701 cacctgaaca gaggtgatat gctcacctca gaacaacaca ggtgctc
//
LOCUS       DAMZII010000275         2726 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZII010000275 DAMZII010000000
VERSION     DAMZII010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.280"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1590
                     /locus_tag="POA77_004857"
     CDS             <1..1590
                     /locus_tag="POA77_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6368164.1"
                     /translation="EYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIV
                     LLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAE
                     MKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGV
                     DERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFG
                     YRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIW
                     IARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDI
                     GFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKEN
                     GLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPD
                     SIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
                     YLYQKPMPFSAWDKSGKLVKE"
     gene            1598..>2726
                     /locus_tag="POA77_004858"
     CDS             1598..>2726
                     /locus_tag="POA77_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6368165.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVI"
BASE COUNT          746 a          530 c          652 g          798 t
ORIGIN      
        1 gagtatattg caaagacatc agcagtgcca aataatgcat tgttcgaaat ctatttttat
       61 tatcctggcg gtaatttatt gaacgcatca gataaacttt tttatctacc gtttgctttc
      121 attattatcg tgttgctggt ggtatatttc atgaccactc gtgtgttccg tcggcaattt
      181 tctgaaatga ccgagctggt taatacgctg gcgtttttgc ccgactcaac ggatcaaatc
      241 gaggctctga aaattcgcga aggcgatgcg aaagagatta tcagcatcaa aaattcgatc
      301 gcggaaatga aagatgccga aattgaacgg tcaaataaat tgctctcact gatctcttac
      361 gatcaggaaa gcggttttat taaaaatatg gcgattattg agtccaacaa taatcagtat
      421 ctggctgtgg ggatcatcaa actgtgtggt ctggaagccg tggaagcggt gtttggtgtt
      481 gatgaacgca ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa atatgcgcaa
      541 tgctgcgata tcgtgacatt caatgccgat ctctatttac ttctgtgtcg ggaaaatgta
      601 cagacattta cccgtaaaat agcgatggta aacgattttg acagcagctt tggctaccgc
      661 aatctgcgca tccataagtc tgccatttgt gaacctttgc agggggaaaa cgcctggagt
      721 tacgcagaaa aactgaaact ggcgatttcc agtatccgtg accatatgtt ctccgagttt
      781 attttttgtg atgacgcgaa actcaacgaa atagaagaga atatctggat tgcgcgtaat
      841 attcgccatg caatggaaat tggcgaacta ttcctcgtct atcaaccgat cgttgatatt
      901 aacacccgcg ccattctggg cgcggaggcg ttgtgccgtt gggtttctgc ggagcggggg
      961 atcatttcac cgctgaagtt cattaccatt gctgaagata tcgggtttat caatgagctg
     1021 ggttatcaga ttattaaaac ggcaatgggt gaattcagac attttagtca gcgtgcgtcg
     1081 ctgaaggatg atttcttact gcatattaat gtttcgccct ggcagttaaa cgaaccgcac
     1141 tttcatgagc gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa cagcctctgt
     1201 gttgagatca ctgaaaccgt gatcgagcga attaatgaac atttttatct caatattgaa
     1261 caactgcgta aacaaggggt acggatatcg attgatgact ttggcaccgg tttgtcaaac
     1321 ctgaaacgtt tttatgaaat taatccagac agcataaagg tggactcgca atttaccggc
     1381 gatattttcg gtactgcggg aaaaattgtg cgcattattt tcgatctggc acgctataac
     1441 cggatcccgg tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga attaattaaa
     1501 ttaggatgtg ttcaggctca ggggtatctg taccagaaac ccatgccatt ctccgcctgg
     1561 gataaaagtg gaaaattagt aaaagagtag tttacgtatg tccagaatca ataagttcgt
     1621 acttacagtc agtctgctga tttttatcat gatttcagca gttgcctgcg ggatctacac
     1681 tcaaatggta aaggaacggg tgtatagcct gaaacagtcc gttattgata ctgcttttgc
     1741 ggtggcaaat attgctgaat atcggcgtag cgtggcaatt gatcttatca acacgctaaa
     1801 tcccacggag gaacagctgt tggttggttt gcgcacagct tacgccgact cggtttcccc
     1861 ctcttatttg tacgatgtcg gtccttatct gatttccagt gacgaatgta ttcaggtaaa
     1921 ggagttcgag aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc gacatgtcaa
     1981 aaatacaggg tttatctctt ttgacggtaa aaccttcgtc tattacctct atccggtaac
     2041 tcacaatcgt agtctgatat ttttgcttgg tctggagcgt ttttctttac tgtcaaaatc
     2101 gctggcgatg gacagcgaga acctgatgtt ctctctattt aagaacggta aaccggtgac
     2161 cggtgatgaa tataatgcta aaaacgccat cttcaccgtt tcggaagcga tggagcactt
     2221 cgcctatttg ccgaccggat tgtacgtatt tgcgtataaa aaagatgttt atttgcgggt
     2281 ctgtacattg attattttct ttgccgcatt ggtggcggtg atatcgggta ccagttgcct
     2341 ctatctggta cgcagagtga ttaatcgtgg tattgtggag aaagaagcca tcattaataa
     2401 ccattttgaa cgcgtactgg atggcgggct tttcttttcg gctgccgatg tcaaaaaact
     2461 ctacagtatg tataactcgg cgttcctgga cgacctgacc aaagcaatgg gcagaaaatc
     2521 ctttgacgaa gatttaaaag cgctgccgga aaaaggcggt tatttgtgcc tgtttgacgt
     2581 cgataaattc aaaaacatta acgacacctt cggtcatttg ctgggcgatg aagtgttgat
     2641 gaaagtggtg aaaatcctta aatcacagat cccggtagat aaaggtaaag tctaccgctt
     2701 cggcggtgac gaatttgcgg tgattt
//
LOCUS       DAMZII010000276         2682 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZII010000276 DAMZII010000000
VERSION     DAMZII010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.281"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(192..410)
                     /locus_tag="POA77_004859"
     CDS             complement(192..410)
                     /locus_tag="POA77_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368166.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(542..2065)
                     /locus_tag="POA77_004860"
     CDS             complement(542..2065)
                     /locus_tag="POA77_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6368167.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2397..2645)
                     /locus_tag="POA77_004861"
     CDS             complement(2397..2645)
                     /locus_tag="POA77_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368168.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMNNTTLTDIIINDRESVLS"
BASE COUNT          827 a          577 c          551 g          727 t
ORIGIN      
        1 tttaacaaat aactcttcga catcaaaatt gttaatagtt gcgatgagga ttatttcggt
       61 gatatataaa aacaaccgtg ataacaccag ggttgacaac tatttgcgca ataatgcctt
      121 tccttcaatg atccaggcgt tcactgccag caacccattc agccgcatac tgacagtgag
      181 aaactgaaag attattgctg atccctgtgg ttgacgagtc tttctcttaa tcgattaacc
      241 agattgatcg ccagccaaac aagggcatta agcaaattta tcgtcacaag cacttctccc
      301 agcccggtac tgggttttgc aataaggatg accaggaggg tgatcagcat aaatgcaatg
      361 gaaaagatga tatagagagt gaaataatcc agattattac tattgttcat gctactaatg
      421 gcaatcctgt caaaactttc aaacaattat agcaaacaaa ataatgaaca atagtaacaa
      481 gaggcaacga gctaattagc tgtctataat tacaccgcac tatggcctta ctgatcaatt
      541 ttcactcaac cacaaccttc accttcggtt tagaaaggat aattttgacc aggtcgatgt
      601 atgtaacagg tttatagaaa taataaccct gctgaaatgt gatattattt tggttcagat
      661 agtcaagttg ttctttcgtt tcgacacctt cagcgacgat actgatcgaa agtttacgcg
      721 ccagatccaa tacacaatcc agaattcggg ttgattctgg gtctgcgttt acgcggccaa
      781 cgaaactgtg atcaattttg atataatcaa tatgcaggtc atgaagataa gaaagccctg
      841 agtagccagt accgaaatca tccagcgcga tgacaaaacc attttcatgc agaatgttca
      901 accgctgaac cagactttca tcaacattca atggctcacg ctcggtgact tccagaacaa
      961 ggtttaaatc gcggcgggta aaactgtcac ggaaatttaa acactcgtcg acaaacgtcg
     1021 gcgaaataat atgcgaagcg ctaaaattaa ttccaatatg aaaaccttcc ggcaatttac
     1081 tcgcgatagc gttcatctgt ctggcaacct gattcatcag gctttgcgta agcggaacga
     1141 ttaatccgga tttttcagca agtggaataa atgccgcggg tgatatatat ccaccgtgag
     1201 gttgtttcca gcgggctaac acctcaactc cccgcaatgt cccttcccga ccatttacca
     1261 caggttggta aaaagggacg atctccccat tatttattgc ccgtcgcagg atctcttcag
     1321 gcgttgcgct tttattgaaa tatctatcaa gcaggaaggc agcggcgcag gcgattaaca
     1381 gaataaatat taaaatccca aagcctttat cgataagtct gtagaagcta aagagtggcg
     1441 gcgggttaaa gataatagtg aaatgatatt ttgtggagtg aaccttctcg gtatagggat
     1501 aatgtccatt aagcgtcatc acatcacccg ttggtccaat aatgtcatcc gccacgcgta
     1561 atacatacct tacccctttc aagggtacat taagtgcccc acgaatatgc tgatcgctta
     1621 tcgtaaccaa aatgcgcgta tctgcaaatt ggctctgata gagcaggata ggtaatctat
     1681 taacggtgtc gattgccgga gccaacagta aattactgtc cgggacaaca ggaatacgag
     1741 acagcaggac ccgattccca ggcagggatg tacaccagac tattccctgt ttgagaatga
     1801 tgattgtgcg caggtgaggt ttcagagctg cttcagtgcc aagctgatat tgctcctcga
     1861 ggtcgcattc cttcccggca atgttaaccg ctgtttgagt cgcctgtgac gcttcttcaa
     1921 gtatatgatt gatattgttg gcggcatatc ttgctcccgc caggtactct gcttttgcgg
     1981 aataccagag ctggatgttc aaaatagcga caccggtaat aaacaagcat atcgcaacga
     2041 ggatgggtat gagtgtattg cgcatttgct tgtcccggcc aaagggttta atacatgaaa
     2101 atgccattca ttcgctcgcg ctggcatcta cagagagcat ggtagagagc ataagaaata
     2161 tccacgccga agataaaatt ttctccatct tgatctaacc cattaattat tgagcataat
     2221 gtaggcagta caaaataagt taggcgggat atcaggcgtc aagaatggag agcgagaact
     2281 ctccattctt gacacctgat attgcggaca taataagaaa gcataacgcc tgaaatgctc
     2341 actttgtatc agcatggtga tacagctgat gtttattcta aaaccttact caagttctaa
     2401 gagagcacgg attccctgtc attaataatg atatccgtta gtgtagtatt attcatttca
     2461 atgtactgca atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa
     2521 cgcaagcgta aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc
     2581 tccctctcga gatttttaaa caaagcacat tgaaaaataa atctctctgg gattgaatta
     2641 ttcatgtcag cctcgtagaa atattaacct aataaaatta tg
//
LOCUS       DAMZII010000277         2657 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMZII010000277 DAMZII010000000
VERSION     DAMZII010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.282"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            33..710
                     /locus_tag="POA77_004862"
     CDS             33..710
                     /locus_tag="POA77_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6368169.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPMVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="POA77_004863"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="POA77_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6368170.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..2648
                     /locus_tag="POA77_004864"
     CDS             1077..2648
                     /locus_tag="POA77_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDI6368171.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          640 a          728 c          738 g          551 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccatg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cggggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggcaaac cgggtacgcg atctgttgcc ctggaaagtt gatctgagct
     2641 ctcagtaaat atcaata
//
LOCUS       DAMZII010000278         2643 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZII010000278 DAMZII010000000
VERSION     DAMZII010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.283"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            3..509
                     /locus_tag="POA77_004865"
     CDS             3..509
                     /locus_tag="POA77_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6368172.1"
                     /translation="MTCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAER
                     LLAGLPAH"
     gene            complement(519..1946)
                     /locus_tag="POA77_004866"
     CDS             complement(519..1946)
                     /locus_tag="POA77_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDI6368173.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1943..>2643)
                     /locus_tag="POA77_004867"
     CDS             complement(1943..>2643)
                     /locus_tag="POA77_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI6368174.1"
                     /translation="ESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGI
                     DARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVAST
                     AECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFA
                     GYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAY
                     YSACPETLKDMSDL"
BASE COUNT          747 a          647 c          600 g          649 t
ORIGIN      
        1 caattacctg tattagccaa cccaagaaaa caacgttgcg tgataaagcc atggtgaatt
       61 atgcctttga ttatttaagc tcaccaggca gtctaccgtt aaccacggca gccacggagc
      121 tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt tatcttcatg
      181 gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag caatcagcag
      241 aacaggaaaa tccgcgcgca caaagcaaac tggggtggat ctacctgaaa ggcctgggag
      301 tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg
      361 ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt aatgttaacc
      421 attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa
      481 gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag atggagtatt
      541 ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac gcccagacag
      601 cccagcccgc catcgtcaac gggcattggc tgcggttgcg gctccggctc gacggtttcc
      661 tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga
      721 caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc tgtcggttgg
      781 ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca
      841 cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc
      901 cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg
      961 ctgtcgccgg ggacataaag tagcggtttt cccttatcgt taaccagatc acgcgcttca
     1021 atcatcatca ccactaatgg gtaataatcg acccgatgct gacggcacca ttgcaacatt
     1081 aaagcatccg cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcaag
     1141 ctttcgcctt ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta
     1201 tcaggttcag cttcccgcca cgaccgggcg taatccagaa gttcggggat atccaggcga
     1261 cctgcgctat accagcggag taaatttctg tgcagttttg catcatcggg gatgatcact
     1321 gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg
     1381 aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc
     1441 tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg
     1501 tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg
     1561 cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca gcgtaattca
     1621 tcaatatctt ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc
     1681 agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat ttcatgttct
     1741 gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc
     1801 gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag
     1861 gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt
     1921 tcgagaatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg
     1981 cagaatagta agccaatggt tgccaggaca aggtagggta atacgcatca tcactggcaa
     2041 ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcgc
     2101 gtaataactg cagatgatca tcttcgggag actgccaata ttgccgacca aaggtataac
     2161 cggcaaagta ttgccgccag ttacgataat aacgtagagc ccgtagatga atgcgcgact
     2221 ggatccatag actttcttct tcgctcaacc agttattaag tacgcccatt cggcttaaaa
     2281 aacccatccg gacataatcc caggctttaa ttcctccctc accacagcat tcggcggtgc
     2341 tggcgacaaa ttgcgcataa gcctgacctt gctcatttag ttcagcaaga tagtcacgcc
     2401 attcacatgg cgaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac
     2461 cgttatcgat cattcggtga attgtgcgca gcaggtcatc acgggcatca attccccagg
     2521 aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggtgatgaa tgcgaaggta
     2581 aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact
     2641 cca
//
LOCUS       DAMZII010000279         2562 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZII010000279 DAMZII010000000
VERSION     DAMZII010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.284"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            65..1495
                     /locus_tag="POA77_004868"
     CDS             65..1495
                     /locus_tag="POA77_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDI6368175.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1536..2468)
                     /locus_tag="POA77_004869"
     CDS             complement(1536..2468)
                     /locus_tag="POA77_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDI6368176.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          798 a          531 c          566 g          667 t
ORIGIN      
        1 gctggacatt atttcagcat aagcggatat ttaaagtttt gctgttttat cagggaatat
       61 ttatatgata cataagtcag ctacaggtgt tattgttgcg ttagccgtaa tctggggtgg
      121 tggcacatgg tacacaggta cgcaaattca gcctggtgtc gaaaagttta ttaaagattt
      181 taacgatgcg aaaaagaaag gtgaacatgc ctacgatatg acgttaagtt ataaaaactt
      241 tgacaaaggc ttttttaatt cccgttttca aatgcaaatg acattcgata acggtgcacc
      301 cgatctcaat atcaagccag gccagaaagt tgtatttgat gtggatgttg agcacggtcc
      361 gttgcccatc acaatgttaa tgcatggtaa tgtcatccca gcactggcag cggcaaaagt
      421 gaacttagtg aataatgaac tgacacaacc gctatttatc gccgcgaaaa ataaatcgcc
      481 cgtggaagcg acattgcgat tcgcgtttgg tggctcattc tctacgacat tagatgttgc
      541 ccctgcagag tatggaaagt tttcttttgg tgagggccag tttactttta atggtgatgg
      601 tagttcattg tctaacctgg atattgaagg caaagtcgaa gatattgttc tgcaattatc
      661 accaatgaac aaagtaacgg caaaaagttt tactattgat tctctggcgc gattagaaga
      721 aaagaaattt ccggttggtg aaagcgagtc gaaatttaat cagattaaca ttatcaatca
      781 cggggaagac gttgcccaaa tcgatgcttt cgttgcaaaa accaggctgg atcgcgttaa
      841 agacaaagat tatatcaatg tcaatctcac ctacgaactt gataagttaa caaaagggaa
      901 tcagcaactc ggtagtggtg agtggtcatt gattgctgaa tctattgatc cctcagcagt
      961 gcgccaattt atcatccagt ataacattgc gatgcagaag cagcttgctg cacaccctga
     1021 gttagcaaac gatgaagttg ctctgcaaga agtgaatgct gcattgttca aagagtattt
     1081 accgttatta caaaaaagtg agccgaccat taaacaaccg gtaaaatgga agaacgcact
     1141 cggcgaacta aatgccaatc tggatatcag tattgccgac ccagccaaat cttcatcatc
     1201 cacaaacaaa gatatcaaat cgctcaattt tgatgtgaag ttaccgctta atgtcgtcac
     1261 agaaaccgca aaacagctta atttatctga aggaatggat gcggaaaaag cgcaaaagcg
     1321 ggctgataaa caaatcagcg ggatgatgac cttaggtcag atgtttcagt taatcacgat
     1381 tgacaacaat accgcctcgc tgcaactgcg ttatacaccg ggtaaagttg tttttaacgg
     1441 acaggagatg agcgaagaag aatttatgtc tcgtgccgga cgttttgttc attaaaatac
     1501 tcactcgcct ctgctcattc agaggcgagc aaaatttact tgtcctgccg ctgtgatgac
     1561 atcaacgacg tcagaagtcg gtctttttcc tgccacagtg agttcagcca ttgctgaaaa
     1621 tggcgcttaa agcttttgtc attgatgtag tcgccatgta actcatcggc aataggctgt
     1681 aaatccacat gaacgacaat tcgcgtcagt ttaccgctta acatatcgaa gaacggctga
     1741 cggttattgt ccggataaca cagcgtaaca ttaagcagtt tatcgaattg tttacccagt
     1801 acatttagcg ccatcgcaat gcctgccgcc tttggtggca acaagttttg aaaagtggag
     1861 tgggtttgct gatgtttttc ttgcgtgaaa cgggagcctt caacgaaatt gacaatggtg
     1921 gtgggatgca ggcgaaactt ttcacaagaa cgccgagtgg tttcaacatc tttaccacgt
     1981 cgctccggat gacgtaataa ataagcgcgg gaataacgct tcataaacgg catatccagc
     2041 gcccagcacg ccaggccaag aaatggcacc caggccagct gttgcttgag gaaatattta
     2101 ttcatcggaa tgtgcttacg aaacagcacg cacagtacga caatatctgc ccaactacgg
     2161 tggttacaaa taagcagata ccagttcttc ttacttaacc cttccagccc gtgaacttcc
     2221 cattgcaggt gtgggttcag atgcagtaat accgccagac cttcacacca gcaatacatc
     2281 ataaaatcac aaaaacgcga aacctttcgc caaatgactg gcacaggtag caatagtttt
     2341 acaatcccgg caatgatgat cgggacggaa caaaaaatag tgaccaaaat ggtcaatacg
     2401 atactcagca aaagggttat cgcagcgagt attctcgtca taatgaattt attcaaaagg
     2461 ttagccatag acagtgcgct aaagaagcgc aaggcgcaga ttttagcaga aaacgaccca
     2521 aataacggat gatccttaag gagaaaaata attcatatct at
//
LOCUS       DAMZII010000280         2551 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZII010000280 DAMZII010000000
VERSION     DAMZII010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.285"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..2487
                     /locus_tag="POA77_004870"
                     /pseudo
     CDS             1..2487
                     /locus_tag="POA77_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          604 a          669 c          894 g          384 t
ORIGIN      
        1 acccgcagcg agtatgatga ggcggggagg ctgaaggcac agacggatgc ggcggggcgg
       61 cggacggagt acagcctgca tatggcgtcg ggtgcggtga cggcggtgac ggggccggac
      121 ggcaggacgg tacggtatgg ctataacagc cagcggcagg tgacgtcggt gacgtacccg
      181 gacgggcttc gcagcagccg ggagtatgat gagaagggaa ggctgacggc ggagacctcg
      241 cgcagcggtg agacaacgcg gtacagctat gatgacccgg cgagtgagct gccgacaggg
      301 atacaggacg cgacgggcag tacaaaacag atggcatgga gccgttacgg tcaactgctg
      361 gccttcacgg actgctcggg gtacacgacg cggtatgagt acgaccggta cggtcaacaa
      421 atcgccgttc accgggagga aggcatcagc acttacagca gttataaccc gcgtgggcaa
      481 ctggtcagtc agagggatgc gcaggggcgg gagacccgtt atgagtacag cgccgcaggc
      541 gacctgacag caacggtatc accggacggt aaacgcagca ccatcgaata tgataaacgc
      601 gggcgtccgg tgagtgtgac ggaaggcggt ctgacgcgca gcatggggta tgacgctgcc
      661 gggcgcatca ctgtgctgac caacgagaac ggcagccagt ccacgttccg gtatgacccg
      721 gtcgacaggc tgacggagca gcgtggcttt gatggcagga cgcaacgtta ccagtatgac
      781 ctgaccggaa aactcacgca gagtgaagat gaggggctta tcaccctctg gcattacgat
      841 gcgtcagacc gtatcacccg gcgcacggtg aacggcgacc cggcggaaca gtggcagtat
      901 gatgaacacg ggtggctaac caccatcagc cataccagtg acggccaccg agtgtcggtc
      961 cactacggct atgacgataa aggccgcctg acgggcgaac ggcagacggt ggagaacccg
     1021 gagacggggg agatgctgtg ggagcatgag acggggcatg cgtacagcga acaggggctg
     1081 gcgacccgtc aggagccgga cggtctgccg ccggtggagt ggctgacgta tggcagcggt
     1141 tatcttgcgg ggatgaagct gggcggaaca ccgctggtcg agtacacgcg ggaccggctg
     1201 caccgtgaga cggtgcgcag cttcggcagc atggcaggca gtaatgcagc atatgaactg
     1261 accagcacat acaccctcac agggcagcta cagagcaggc acctgaacct cccgcagctt
     1321 gaccgggact acgactggaa cgacaacgga cagctaatcc gcatcagcgg cccgcaggag
     1381 agccgggagt accgttacag tgacacggga aggctgacgg gcgtccacac cacggcggcg
     1441 aacctggata tcgatatccc gtatgcgacg gacccggcag gaaaccggct gccggacccg
     1501 gaactgcacc cggacagcac gctcacggcg tggccggata accgcatcgc ggaagatgcg
     1561 cactatgtct accgctacga tgaatacggc aggctggcgg agaagacgga ccgcatcccg
     1621 gaaggggtga tacggatgca cgacgagcgg acccaccact accactacga cagccagcac
     1681 cgtctggtgt tctacacgcg gatacagcac ggtgagccgc aggtggagag ccgctacctc
     1741 tatgacccgc tgggccgccg gacgggaaaa cgggtgtggc ggcgggagcg tgacctgacg
     1801 gggtggatgt cgctgtcgcg taaaccggag gagacctggt acgggtggga cggtgacagg
     1861 ctgaccactg tacagaccca acagacaaga atccagacgg tataccagcc gggaagcttc
     1921 acgccgctcc tgagaatcga aacagagaat ggtgaacagg cgaaggcgcg gcaccgtagc
     1981 ctggcggagg tgttgcagga ggacacgggt gtgacgctac cggcggagct ggcggtgatg
     2041 ctggggaggc tggagcggga actgcggcag ggcagcgtga gtgaagaaag ccagcagtgg
     2101 cttgcgcagt gcgggctgac ggcggaacag atggccgcgc aactggaggc ggaatacatc
     2161 ccggagagga aacttcatct ttaccactgt gaccaccgtg ggctgccgct ggcgcttatc
     2221 agcccggaag gggaaacggc gtggcagggg gagtatgacg agtggggaaa cctgctgggc
     2281 gaggaaagcg cgcagcacct tcagcagtca ctccgtctgc cgggacagca gtatgatgag
     2341 gagtcggggc tgtactacaa ccgcaaccgg tactatgatc cgttgcaggg gagatatatc
     2401 acccaggacc cgatagggct gagaggtgaa tggaatctgt ataaatatcc attaaaccct
     2461 gttcggttta tagattcttt gggtttaaaa ttccatgtta acggtgatcc aagtgatttt
     2521 aatcaggctg ttgagtattt aaaacaagat t
//
LOCUS       DAMZII010000281         2519 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMZII010000281 DAMZII010000000
VERSION     DAMZII010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.286"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            14..2389
                     /locus_tag="POA77_004871"
     CDS             14..2389
                     /locus_tag="POA77_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HDI6368177.1"
                     /translation="MYIEMKETFLELPSEIQFSEAANAAILTQDKWGDVWQTLKTDAD
                     LNYTDAGEPVSLASRVTTATSAIYHAITEGWTMCVGYSGGKDSHSLLHLFLMALIRAV
                     RNGSNVSQHHFIQMSDTLIENPEMHHQATQVLSQLRAFIAEQELPLTVLVARPSMTQS
                     WVGRILTGRGLPTWTNSSTRQCATDLKRSPLRQAKARYLKNAPASVRNKVCLMLGSRD
                     DESARRADNILRMGGQADSVSLTEHGGELYPVKEWRAQDIWSFLMACGSLARFPLPSF
                     LPDNFSLATLYKDATGECIWTPEKSTVRSSACGARYGCSLCCAVGIDHSMETLLRTDP
                     EKYGYQAGLNRLQRFLTKIQYDWSLRDYIGRKVFEGGYVRLQPNVFSSSLTERLFHIC
                     CSLDYVEAQRAAAVHEKLLSGEIEDTAHNRRMSEPQFRLVQEANVLHIDFLWSLHCFN
                     PRPFRAIELYRQVWEGGALDLLDDEPAMQPVPRTPMPAPRWMKLPEGRIGTSFDGLSD
                     PSSEIAYFDGREDERASRSLMSSGEGLNIVAFEEEDELTVDEDTASWIIWHEYDGLRQ
                     RVSDGEFTPVAAAQYLLRYGAVRISRGKGAVYHRLAQRGQAYSRLGINDQTSLPSLLA
                     SRRFKILTDSDYRLLVARKLRGQRQKLRFWCCVAACVALHTYNKTPLGGWITTQLETE
                     QQLHLARTGDELKARTLDAVLTLCNLRISSQQPEERLYYRAVRKRFLETLAGCISPER
                     EGTLREVIWELRMLSGPQSSKKTGFRHVDECRERTAALLRPLLKRMVRLLA"
BASE COUNT          567 a          679 c          722 g          551 t
ORIGIN      
        1 ttcaggaaat aatatgtata tcgaaatgaa agaaacattc cttgaattac cctcagaaat
       61 tcaattttct gaggcggcta acgctgccat tctgacgcag gataaatggg gcgatgtctg
      121 gcagacactg aagaccgatg cggatctgaa ttatactgat gccggtgagc cggtcagtct
      181 cgcttcgcgc gtgacgacgg caaccagtgc catttatcat gcgattaccg aaggctggac
      241 catgtgcgtc ggctacagcg gcgggaaaga ctctcactcg ctactgcact tgttccttat
      301 ggccttgatc cgtgcagtac gaaacgggtc gaacgtcagc cagcaccact tcattcagat
      361 gtctgacacc ctgattgaaa accctgagat gcatcatcag gccacgcagg ttctcagcca
      421 gctcagagcc tttattgccg aacaagaact gcctttgacc gtccttgtgg cccgcccgtc
      481 catgacccag agctgggtcg gacgcattct gaccgggcgt ggacttccaa cctggacaaa
      541 ttcttctacg cgtcaatgcg ctacagatct aaagcgttct ccactgcgcc aggccaaagc
      601 ccgttatctg aaaaatgcac ctgcttctgt ccgcaacaaa gtgtgcctga tgctgggttc
      661 ccgcgatgat gagagcgccc gtcgggcaga caacatccta agaatgggcg ggcaggcgga
      721 cagcgtctcg ctcacggagc acggcggcga actgtacccg gtgaaagagt ggcgggcaca
      781 ggatatctgg tcatttctga tggcctgtgg ttctctggcg cgatttccgc tgccaagctt
      841 tttgccggac aacttcagcc tcgccacgct ttacaaagac gcgaccgggg aatgtatctg
      901 gacgccggag aaatccactg tccggtcttc tgcctgcggc gctcgctatg gatgctcgct
      961 atgctgcgcc gtcggcattg atcactcgat ggaaacctta ctccgcactg accctgagaa
     1021 gtacggctac caggctggtc tcaaccgcct tcagcgtttc ctgaccaaaa ttcagtacga
     1081 ctggagcctt cgggattata tcggacgcaa agtgtttgag ggcgggtatg tgcgtcttca
     1141 gcccaatgtg ttctcgtcgt ccctgaccga aagactgttc catatctgtt gcagcctcga
     1201 ttacgtggag gctcagcggg ccgcagcggt gcatgaaaag ttactttcag gcgagatcga
     1261 agataccgcg cataaccgcc gcatgtcgga gccgcagttt cgactggtgc aggaggctaa
     1321 cgtgctgcat atcgactttc tctggtcact gcattgcttt aacccgcgcc catttcgtgc
     1381 cattgaactt taccgccagg tgtgggaagg gggggcactg gatcttctcg acgatgagcc
     1441 agctatgcaa ccagtacccc gcacgccgat gccagcacca cgctggatga agttgccgga
     1501 aggccgtatc gggacgtcat ttgacgggct gtccgatccg tcatccgaaa tagcctattt
     1561 tgacggtcgg gaggatgagc gagcttcccg cagtctgatg agtagcgggg aaggcctgaa
     1621 catcgttgca ttcgaagaag aggatgagct gacggtcgat gaagacacgg catcctggat
     1681 tatctggcat gaatatgacg gattacgcca gcgtgtgtct gacggtgagt ttacccctgt
     1741 ggcggcggca cagtacctgc ttagatacgg cgccgtccga atttcgcggg ggaaaggtgc
     1801 ggtgtaccat cgactcgctc aacgcgggca ggcctattca cgtcttggca ttaacgacca
     1861 gacgtcgctg ccgtctctgc tcgccagccg ccgtttcaag atcctgaccg acagcgatta
     1921 tcgcttgctc gtagcccgca aactgcgggg gcagcggcag aaactgcgct tctggtgctg
     1981 tgttgcggcc tgtgtggcac tgcacaccta caacaaaacc cctctggggg ggtggataac
     2041 gacgcaactg gagacagaac aacagctaca tctggcccgg acgggagacg aactgaaggc
     2101 aaggactctt gacgctgtcc tgaccctctg taacctgcgg atttcgtcac agcaaccgga
     2161 agaacgtctg tattaccggg ccgtaagaaa acgctttctg gagacattag ccgggtgtat
     2221 ttcgccggag cgcgaaggaa ccttacggga agtcatctgg gagctgcgga tgttatccgg
     2281 ccctcagtct tcgaaaaaga cggggttccg gcatgtggat gaatgccggg agaggacggc
     2341 ggcgttgtta aggcctctgt tgaaacgtat ggtccgcctg ctggcgtgag cccaccagca
     2401 aaatcagaca tacgtgcaaa aaacgtgaaa atagccccgt taacggaaaa agcgccgaat
     2461 ggtgtgtgtt gtaatgagcg caggtgcgga attcgttaaa gggactgttt tgaaaagta
//
LOCUS       DAMZII010000282         2486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMZII010000282 DAMZII010000000
VERSION     DAMZII010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.287"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(104..1294)
                     /locus_tag="POA77_004872"
     CDS             complement(104..1294)
                     /locus_tag="POA77_004872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6368178.1"
                     /translation="MAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRI
                     QTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGLLDRL
                     EGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISE
                     DGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYI
                     TPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITN
                     SAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQ
                     PAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKTKQGSSD
                     ASNY"
     gene            complement(1263..>2486)
                     /locus_tag="POA77_004873"
     CDS             complement(1263..>2486)
                     /locus_tag="POA77_004873"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017411.1,HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6368179.1"
                     /translation="HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGAVTAVTG
                     PDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLTAETSRSGETTRYSYDDPASE
                     LPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRYGQQTAVHREEGISTYSS
                     YNPRGQLVSQRDAQGRETRYEYSAAGDLTATVSPDGKRSTIEYDKRGRPVSVTEGGLT
                     RSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSED
                     EGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDDKG
                     RLTGERQTDGEDGPHPDGCYPDGRRADPPLPLRQPAPPGVPHADTAWRATGREPLPLR
                     PAGTANGETGMAAGA"
BASE COUNT          454 a          809 c          598 g          625 t
ORIGIN      
        1 tgttttatgt ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac
       61 aacaaataaa caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat
      121 ctgaactccc ttgctttgtt tttattggat tatctgcttt tataccatct aacccgaaga
      181 gttgcccagt gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac
      241 aagcaatacc gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt
      301 gtctatcccc tgccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg
      361 tcttagtcat tcgacacatc aagtaacaat gtctcattgc atcggcggcc ccattatgag
      421 ctccgccaac aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt
      481 ttgcagcaat caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat
      541 tctcaatcgc atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat
      601 acatattcca tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg
      661 gatcgtagta ccggtgacgg ttatagtaca gccccgactc cttatcatac tgctgccccg
      721 gcagccggta cggctggtgc aggtgatgcg ggttctcctc attaagctgg ttgccccatt
      781 catcatattc cgcgctccac gccgtattgc cgtcttcact gataagcgcc agcggcaggc
      841 cccggtggtc acagtgataa agatgtattt ttcgctcagg caaataaacc ggctctatct
      901 gtgccgccag ccgctccacc gtcagcccgc actgcgcaag ccactggcgg ctttcactgc
      961 tcacgcagtt tgcccggatt tccccctcca gcctgtccag cagccccacc agttcagccg
     1021 gaaataccac gccgtgcccg tcctcgctcc cttcctgctg tatcttctcc gccaggctgc
     1081 ggcactgcgc tttctcccgt tcaccgttgt ctgtttcgat gcggatgagc ggcgcgaagc
     1141 tccccggctg gtataccgtc tggatacgcg tggtgtcggt ctgtaccgtc gtcagcctgt
     1201 cgccgtccca gccgtaccat gtctcctccg gtttacgcga cagcgacatc caccccgtca
     1261 ggtcacgctc ccgccgccat acccgtttcg ccattcgccg tcccagcggg tcgtagaggt
     1321 agcggctctc gaccagtggc tcgccatgct gtatccgcgt gtggaacacc aggcggtgct
     1381 ggctgtcgta gtggtagtgg tgggtccgct cgtcgtccgt ccggataaca cccgtcggga
     1441 tgcggtccgt cttctccgtc agtctgccgt tcgcccgtca gacggccttt atcgtcatag
     1501 ccgtagtgga ccgccacacg gtggccttca ctcaggtggc tgatttctgt cagccagccg
     1561 tggtcgtcat actgccactg ctccgccggt tcaccgttca ccgtgcgccg ggtgatacgg
     1621 tccgacgcat cgtaatgcca gagggtgata agcccctcat cttcactctg cgtgagtttt
     1681 ccggtcaggt catactggta acgttgcgtc cggccgtcaa agccgcgctg ctccgtcagc
     1741 ctgtcgaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg
     1801 atgcgcccgg cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc
     1861 ggacgcccgc gtttatcata ttcgatggtg ctgcgtttac cgtccggtga taccgttgct
     1921 gtcaggtcgc ctgcggcgct gtactcataa cgggtctccc gcccctgcgc atccctctga
     1981 ctgaccagtt gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga
     2041 acggcggttt gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc
     2101 gtgaaggcca gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg
     2161 tcctgtatcc ctgtcggcag ctcactcgcc gggtcatcat agctgtaccg cgttgtctca
     2221 ccgctgcgcg aggtctccgc cgtcagcctt cccctctcat catactcccg gctgctgcga
     2281 agcccgtccg ggtacgtcac tgacgtcacc tgccgctggc tgttatagcc ataccgcacc
     2341 gtcctgccgt ccggccccgt caccgctgtc accgcgcccg acgccatatg cagccggtac
     2401 tccgtccgcc gccccgccgc atccgtctgc gccttcagcc tccccgcctc atcatactcg
     2461 ctgcgggtga tgctcccgtc ggcatg
//
LOCUS       DAMZII010000283         2477 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMZII010000283 DAMZII010000000
VERSION     DAMZII010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.288"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            59..367
                     /locus_tag="POA77_004874"
     CDS             59..367
                     /locus_tag="POA77_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDI6368180.1"
                     /translation="MQINKVMSLLDILTSWLEDNINMESDLFFDNDVDNTTSESLYPA
                     VEKANAVLSKLASLSSETIQATRQRLQDAVEGKGEVSTADVKELLLATRYLMLTTDGE
                     "
     gene            372..863
                     /locus_tag="POA77_004875"
     CDS             372..863
                     /locus_tag="POA77_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3085 domain-containing protein"
                     /protein_id="HDI6368181.1"
                     /translation="MTTLTFEIAGVKKLLEELRSAERFNATIEQLFEPSNYPGGTPLN
                     EEGKTEVEMNQTGGIFWPSSKYIDPARLTPQILLVKDHGVYLITNASLDGTPVSRDTV
                     VYARGMNPSVDDEWYDEAEEALGGDDSSVSIPVAWFELALKKKFNAFSIKVSPTKITL
                     VNG"
     gene            952..1213
                     /locus_tag="POA77_004876"
                     /pseudo
     CDS             952..1213
                     /locus_tag="POA77_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1609..1815
                     /locus_tag="POA77_004877"
     CDS             1609..1815
                     /locus_tag="POA77_004877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368182.1"
                     /translation="MKLFYSKPVRFIVIICVFLLGVDNLSDMFVDISRPVFYGGLAAL
                     LAAAVVIARVDYNKNAEKKASQVQ"
     gene            1869..2093
                     /locus_tag="POA77_004878"
     CDS             1869..2093
                     /locus_tag="POA77_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368183.1"
                     /translation="MHNLEISTIAIFSAQYKNIEEAENAGALYSVDIEYPMTLNDLSL
                     LCESVAKAVGVPNGVKYQFVTQPQPEESDY"
     gene            2128..2334
                     /locus_tag="POA77_004879"
     CDS             2128..2334
                     /locus_tag="POA77_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368184.1"
                     /translation="MGHVDYTRTLRVQLYDASRFHDGATAEQAGELHTVAFSKPAIAD
                     DIQKIVDTTAEVLGKRYSVNVFSN"
BASE COUNT          657 a          586 c          581 g          653 t
ORIGIN      
        1 tattagccag acgagacagt ttgttatatt tgaattacat caaccgaggg cgagaaaaat
       61 gcagattaat aaagttatgt ctttacttga tattcttacc agctggctcg aagacaatat
      121 taatatggag tctgacctgt ttttcgataa cgatgtagac aataccacct cagagtccct
      181 gtatcccgct gtggaaaaag ctaacgctgt attgagcaaa ctggcgtcac tgtcgtcaga
      241 gacgattcag gcgacgcgtc agcgtttgca ggacgcggta gaaggcaaag gggaggtttc
      301 aactgctgac gtcaaagagt tgctactggc cacccgatat ctgatgctga ctacggatgg
      361 agaataagtt tatgaccaca ttaacattcg aaattgctgg ggtaaaaaag ctcctcgaag
      421 aactccgctc ggcggaacgg ttcaatgcaa ccattgaaca gctttttgaa ccgtcaaatt
      481 accccggcgg tactcctctc aacgaagagg gcaaaacaga agtggaaatg aaccagacgg
      541 ggggaatttt ctggccatca tccaaataca ttgacccggc ccgcctgact ccacagatcc
      601 ttctggtaaa agatcatggc gtctatctga ttaccaatgc atctctggat ggaacccctg
      661 tttcccgtga tacggttgtc tatgcccgtg ggatgaatcc gtctgttgat gatgaatggt
      721 atgacgaggc agaggaggca ttgggcggcg atgatagttc ggtctctatc ccggtggcgt
      781 ggtttgaact cgctcttaaa aagaaattta atgcttttag tatcaaagta tcccccacaa
      841 aaattactct ggtgaatgga taaccacggc acaggctggc tatctcgctt aaacagtccg
      901 tccctgatct attaacaggg cggaccttat ttaagacaca ggacactaaa aatggttatt
      961 gacgctgaga attcggaaat gcatcaagta cgtgatttta tcgctgcaaa ctggagcctg
     1021 tttacttcgc acatccgcga gaaaagcatg actgatgaac aggccgaaga ggaagctcaa
     1081 cgcatttttg ttgttgtcgg gggggggaag agtaaaggca tttgcctcgc tcagggattc
     1141 agccaggtgc tggatatgca cgttattttc aggtctgtcc gctaatctgt cagccggaca
     1201 gccctcgtta taaaaggaac attatggcag acacaaaacg tcccagctcc ccgcttacac
     1261 aggcccaaat ctatgtgcta cgtcgcctgg catccggaac gacatgcaag tttttttgac
     1321 tttcccgcgc ggaacccaat tcactgattt gtgccctgca taagaataac ttgccgccgg
     1381 acatatcgtc ggtggaaaag ttacttttct atatttcgat tgtgttctac agcatcgttc
     1441 ttttcctgag ttcaggaagt gacaggccat aggcacctcg aaaatactct cgtcattgcc
     1501 tccctgatgt gtcttttttc ttgtcaaaat aggcccgtta ccgaaaaaaa attggcatct
     1561 gatatggtgt aatgctggtg tcatttcacc ccagaaggat agctcctgat gaaattgttc
     1621 tacagcaaac ctgttcgctt tatcgtcatt atctgcgtat ttctcctcgg cgttgataac
     1681 ctgagcgata tgttcgtcga tatctcccga ccagtattct acggcggtct cgctgcactc
     1741 ctggccgctg ccgtcgttat tgcccgcgtt gattacaaca agaacgcgga aaaaaaggcc
     1801 agccaggtcc agtaaccttt attcagccac cttacggtgg cttctttttt attaacaagg
     1861 actgactcat gcataacctt gaaatctcta ccatagccat tttcagtgcc cagtataaaa
     1921 atatcgaaga ggccgagaac gccggggcct tatattccgt cgatatcgaa tatccgatga
     1981 ctctgaatga cctgtcgctg ctgtgtgaat ctgtagcaaa agccgttggc gtccctaatg
     2041 gggtgaagta ccagtttgtc acccagccgc aaccggaaga aagtgattat taacgcgtat
     2101 agcgcctgac tgaacaggag aggaataatg ggccatgtcg attatacccg gacactccgg
     2161 gtccagcttt acgatgccag ccgctttcat gatggtgcga cggcggaaca ggctggtgaa
     2221 ctgcacaccg tcgcattcag taaacccgcg attgccgatg atattcagaa aattgtcgat
     2281 accactgccg aggtgctcgg taaacggtac agcgttaacg tattctcgaa ctgagcgcca
     2341 gtgaaggagg tattcagaaa acctccttct ttctatattt tatatcaaaa accgtcaaat
     2401 ttaattccgc cgccagccat gttcggcaag cttaaaataa attcattcta ttttccgggt
     2461 gccattttta ggtgatg
//
LOCUS       DAMZII010000284         2428 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMZII010000284 DAMZII010000000
VERSION     DAMZII010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.289"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            80..568
                     /gene="eco"
                     /locus_tag="POA77_004880"
     CDS             80..568
                     /gene="eco"
                     /locus_tag="POA77_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDI6368185.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(717..2363)
                     /gene="mqo"
                     /locus_tag="POA77_004881"
     CDS             complement(717..2363)
                     /gene="mqo"
                     /locus_tag="POA77_004881"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6368186.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          572 a          667 c          608 g          581 t
ORIGIN      
        1 attaataaat ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat
       61 gaagaaggaa agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc
      121 taccacttcc gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc
      181 acaagctgaa aaagggatga agcgtcaggt gattcagtta accccgcaag aagatgaatc
      241 taccctgaaa gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg
      301 tctcggcggg aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt
      361 tgataaagtt agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa
      421 gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc
      481 gatcgtggtg tatacgccag acaatgtaga tgtgaagtac cgcgtctgga aggcggaaga
      541 gaaaattgac aacgcggtag ttcgctaaaa tgctgtgaag tgcggcaccc cgtaggccga
      601 ataaggcggt cacgccgcat ccgacatcta acgcccgagc cggtgcctga tgcgacgctg
      661 gcgcgtctta tcagcatacg ccacatccgg cataccatgc cggatgtggc acatcattac
      721 aacgcaatat ccgccacttc tttttgaacg ggttgcggtt tcaactgcgg ttttggcgta
      781 ctatctgctg cctgcggctt gtcgtatttc agccccagca cttcgctggt gtactgcaac
      841 tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc cataagacgg aacgatcgct
      901 ttcaacgtag cctgccattg cgggctggaa acgcgatcgc caaatacttt ttccagcaga
      961 tccaacataa tcggcgcggc ggttgacgcc cctggcgatg cccccagcag cgcggcaatg
     1021 gtcccttgct ggtcactgac cacttcagta cccagacgca gtacgccacc tttctcggca
     1081 tcacgcttga taatctgcac gcgctgcccc gcttgccaca aacgccagtc ctcttttttt
     1141 gcctgcggat agtactcttt caacgcttca aaacgatcct cttcactcaa catcacctga
     1201 ctcaccagat atttcaccag atcgaaatta tccagcccga cgtgcatcat cggcatcacg
     1261 ttagaggtgg tggtggaact catcagatcc cacaatgaac cgtttttgag gaatttggtt
     1321 gagaaggtgg caaatggccc aaacagcact acgcgtttac cgtccagaac gcgggtatcg
     1381 atatgcggaa ccgacatcgg tggtgcgcca acggatgctt taccgtaaac cttcgccaga
     1441 tggtgattaa ccacgtccgg gttttccgaa acaaggaact gtccgcccac cgggaaaccg
     1501 gcgtagtctt tcgcttccgg aatccccgat tcctgtaata gcttcagcgc cgcaccgccc
     1561 gcgccgataa agacaaattt ggcgcggatg ttctgtgcag tgccgttttt cagatcggca
     1621 acggtaacgg tccaggtgtt atcgtcatta cgctttaggg cgcggacttc gctgctgagt
     1681 tgcagcgaga agttagattt cttctgtaag gaagcaatta actggcgggt gatctcgcca
     1741 tagttcacat cagtgccaat ttctgtgcgt gttgccgcta ctttctgttg tggatcgcgc
     1801 ccttccatca ctaacggtgc ccactctttg atctgcgcgt gatcttcaga gtaacgcata
     1861 ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg cgcgcaggaa attaacgtta
     1921 tcctcgcccc agacaaagct catatgcgga acggtattga taaatgaacg cggagtacgc
     1981 agcacgccgc gctcaacctg gtgcgcccag aactggcggg aaatctgaaa tgcttcgtta
     2041 atggcgactg ctttttcaat actgatgctg ccatcggcgt tttgcggggt gtagttcagt
     2101 tccatcagtg cagaatgccc ggttccggcg ttattccagc cgttcgaact ctcctgcgcg
     2161 acaccctcca ggcgctccac catggtcatc gaccattcag gctccagctc gcgtaaatag
     2221 gtccccaacg tggcgctcat aatgccgccg ccaatcaaca gtacatcagt ttcctgctcc
     2281 tcggacgctt tcgcttttgc cgccatcgaa acggcattaa gccccacggc catcgagaag
     2341 agcatggcag tcactttttt catcttgtta atgccttact tttagtcgct ttattgcagg
     2401 tgagatttgc gcggcatcaa cggtaaca
//
LOCUS       DAMZII010000285         2428 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMZII010000285 DAMZII010000000
VERSION     DAMZII010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.290"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..256
                     /locus_tag="POA77_004882"
     CDS             <1..256
                     /locus_tag="POA77_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HDI6368187.1"
                     /translation="ESEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEIT
                     ELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHGK"
     gene            361..1203
                     /locus_tag="POA77_004883"
     CDS             361..1203
                     /locus_tag="POA77_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDI6368188.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1231..2189
                     /locus_tag="POA77_004884"
                     /pseudo
     CDS             1231..2189
                     /locus_tag="POA77_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2201..>2428
                     /locus_tag="POA77_004885"
     CDS             2201..>2428
                     /locus_tag="POA77_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6368189.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDK"
BASE COUNT          803 a          409 c          563 g          653 t
ORIGIN      
        1 tgaaagtgaa ggtaaatata ccgttagaaa tttggttaaa aataaagcta tagcattgga
       61 attagctgag atatatgtta agaatcgtta tggacaggat gccgcagaag aagaaaaacc
      121 atacgaaatc actgagttaa caacaagttg ggttgttgaa ggtaccattc actcagacca
      181 aattgctggt ggggttttta ttatagaaat aggcaaaaat gatgggagaa ttctgaattt
      241 tggccacgga aaataagtat gtaaggataa tcaaacatcg tgtgcgctga tggcagagta
      301 ggtggaggac tccagacagt caaacgatag aaaaagatag cctttatgga ggttcctgca
      361 atgtcaaata cataccagaa aagaaaggca agtaaagagt atggtttata taatcaatgt
      421 aagaaactaa atgatgatga attatttcgc ttacttgatg atcacaattc cttgaaaagg
      481 atttcatctg ccagagtatt acagttaaga ggtgggcaag acgctgttag attggcaatt
      541 gagttctgct ctgataaaaa ttatatccgt agagatatcg gagcatttat actcgggcaa
      601 ataaaaattt gcaaaaaatg cgaagataat gtttttaata ttttgaacaa tatggcattg
      661 aatgataaga gcgcttgcgt tcgagctacg gcaatcgagt caacggctca gcgatgcaag
      721 aaaaaccaaa tttattcacc taaaatagta gaacaatctc aaattactgc ttttgataaa
      781 tcgactaatg tcagacgtgc tacagcattt gctatttctg ttatcaatga taaagcaaca
      841 attccactat tgattaatct gttaaaagat ccaaatggag atgtcagaaa ctgggccgca
      901 tttgcaataa atatcaataa atatgataat agtgatatta gggattgttt tgtggagatg
      961 cttcaggata aaaatgagga agtccgtatt gaagcaataa tcggactttc ctacagaaaa
     1021 gataaaaggg ttttatctgt tttatgcgat gagttaaaaa aaaatactgt ttatgatgat
     1081 atcattgaag ctgcgggtga attaggtgat aaaacgctac ttcctgtttt agatactatg
     1141 ttgtacaagt ttgatgacaa tgaaattata acttccgcta ttgataagct gaagcgttca
     1201 tagcgtgagt tgcctatgca cagtggggga ttcccgccgg cacggtgcag atgctcgacc
     1261 ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat
     1321 cgtgcggcct gaccgtggag cagatgaaaa accagatgga cccggtgtac acgccggcgc
     1381 gaaaaatcca cctgtaccac tgtgaccatc gcggcctgcc gctggcgctc atcagcacgg
     1441 aaggggcaac agcgtggtgc gcagaatatg atgaatgggg caacctgctg agtgatgaga
     1501 acccgcatca tctgcagcag ctcattcgcc tgccgggtca gcagtatgat gaggagtccg
     1561 gcctgtatta caaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
     1621 atccgattgg gctgaagggg ggatggaatt tttatcagta tccgttgaat ccggtcataa
     1681 atgtagatcc gcaaggtttg gttgatataa atttataccc cgaaagtgat cttatccatt
     1741 ctgtagctga tgagattaat atcccaggcg ttttcacaat cggggggcat ggtaccccca
     1801 catctattga atccgcaacg cgcagtatca tgacagctaa agatctagca tatctaatta
     1861 aatttgatgg gaattataaa gatgggatga cagtttggtt attttcttgt aatacaggta
     1921 aaggacaaaa ttcatttgct agccaattag ctaaagagtt acatacaaat gtaataggac
     1981 ctgacacgct atggacgtgg tgggggcgag gaactaatgg taagttaaaa atggatacag
     2041 tgctaacagc accaacgaac cttaattcaa ataaggatct aatggctata acaacaaaag
     2101 accttggtaa ttggataaca tatgggccat ctgggcaccc catttctaat atgcaaggta
     2161 cgccagaaaa acccagtgat ataagatagg ttgtagatgt atgaaagcat gcttgttact
     2221 atttttttat ttttctctta ttggtcaatt gcatggtgct gatgtgaaaa taaaagaaaa
     2281 cgaaagtgtg atgggatcta cagcaatgac ctatgattta agtgaagaaa agctgatgaa
     2341 actgaagtat aaatcacaac atggtgattc agaggcatca tttcgactat atcaatatta
     2401 ctgctttact aaaaataata ttgataaa
//
LOCUS       DAMZII010000286         2354 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMZII010000286 DAMZII010000000
VERSION     DAMZII010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.291"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(273..1460)
                     /locus_tag="POA77_004886"
     CDS             complement(273..1460)
                     /locus_tag="POA77_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HDI6368190.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMIDFSSEKIHDYIDIIIEDYENIKENAYLVIPQINYTMD
                     LNIEDSSSEELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDLL
                     NAI"
     gene            complement(1482..2021)
                     /locus_tag="POA77_004887"
     CDS             complement(1482..2021)
                     /locus_tag="POA77_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368191.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCVKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(2277..>2354)
                     /locus_tag="POA77_004888"
     CDS             complement(2277..>2354)
                     /locus_tag="POA77_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDI6368192.1"
                     /translation="HLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          703 a          398 c          427 g          826 t
ORIGIN      
        1 tgtacgtaat aaaccgttcc ggcgggtttc agattgttga gtgcgcttta ttcatgccgg
       61 atgcggcgtg aacgccttat cctgcctaca aaagcacgca aattcaatat attgcagaga
      121 tggcgtaggc ctgataagcg tagcgcatca ggcatttttg cggttgccat cagtttcaaa
      181 cccgcgtcag tgagtttttg cttttggtgc acagggtaag atgaataaat atctacggta
      241 ttaataatag actttgtaag aagttattaa gtctatattg catttaaaag gtcgtaatta
      301 tacgtatagt cctctctggt aaaatattga ttctctgagt tatatgcttc atatgtgtta
      361 tattccccga cctcaaaacc attgtccgga gaattttcgt ctttctcgag ggtgttatct
      421 gaaagtaact cttctgagct agagtcttct atgtttaaat ccatagtata attaatttgg
      481 ggaattacca gataagcatt ttcttttata ttttcataat cttcaatgat tatgtcgata
      541 taatcatgta ttttttctga tgagaaatct atcatctctg gtaatttaat tctgttcaag
      601 caattattga ttttcgatga gcttttatat atttcttttg ttaattcatt ttctaattta
      661 tctgtaagag ttgctcttgg tatagatttt ataattgcaa atctcatgat ttttaattgt
      721 tcttcggtaa tatctccaag ctcatttaaa caactttcga caagcttaat tttttcttca
      781 gaatacatat cgctaatgct tgttagaaca ggcgttattt ttagtaaata atcccaggag
      841 attgtggtgt tttgttttga actaacgaaa tttacctgaa acatgttggc attagtaaaa
      901 tctgtgtcat ccaatttaaa aggatttaaa attgcattgg ccatatgttt atctgtaaaa
      961 tcataaagtt ctaaatcgat attccgtaag tccagagagg atagatcaac tatacctcca
     1021 gtcccacaac taaaaactaa gttcaacctc ctgatttcat tgtaaatgtc tttcaatggg
     1081 aaattaacat tgatattgcc tgtggatatt tcctggtttt ttaacggttc attaattgta
     1141 agttcacagg tattgtctga tcgttgaata attagggcgt tataaccaaa aaaatccata
     1201 cgaagagacg aatcattata aataattaca ttttgtaagt acgctaattc tttttgaaaa
     1261 tcaccagtga taatgcattt tccacctggt tgtaacgcgt taagtaaagt gaccatttga
     1321 atagaggtct gttttagttt ttctatacct ggtgctagtg cttcgaggtt attaactatg
     1381 gggctttcag gaagatcatt catttttcga cggactaaat cactactaat atcattacca
     1441 attgatgaaa cagggaacat tattttatcc tttataaata atcaataaaa atcattatta
     1501 aaatgatcgg gtgaaaaaaa gagaatttgc caacaatctg aagtaatgct ggttttatta
     1561 aagtgcgcag gaggtgttaa gatggcttct tttcgggtga attttacaca atacttagaa
     1621 atcgtctcct taatacattc cagctctttt ttattgacgg ctgagcaagt acattgttca
     1681 ccaaaaggtg caaaagtgcc tacatctaat gtttgtatga tataatcgtc atgttctcct
     1741 gtagaaatat ctatgatgaa tggactcata ttttccggtt ggattcggat catgacagaa
     1801 ggaacatcgc cttgatagaa attcaatgca gtaattgcag ataatactct ccgcagttca
     1861 ttacttatac tgtaaatttt attaacgtca ttggtggaga caatttctgc aaactttctt
     1921 tttaccgtcc aataatcaat atttctattg tgattgttaa ccactgcgtg cattggcggg
     1981 attgaagggt gaaaaatatg tgcaattggg aagcctttca ttgatgcctg ccttgtcgtc
     2041 atgacaaaca ataatagatg cttattttcc tcgcagggtt ctttcgcact atcaaaaaac
     2101 gctcatttac ccggcgttac agtaaacgaa gcaacattct gtggcctacg acgatcaaaa
     2161 gctctgtgaa aatttccctt gttgtctttg tctgacctta tcgcggcgat cagcaattat
     2221 tcgttttgtg atcctcaaca caaaatgcta ttcgtcgcct atgaggggag aagaatttat
     2281 ggaaaacgat tgcattcaca aaatgacgtc ctgtcagaag agaaaaagtt gacgttaatc
     2341 aattgttcga gatg
//
LOCUS       DAMZII010000287         2335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMZII010000287 DAMZII010000000
VERSION     DAMZII010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.292"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..294
                     /locus_tag="POA77_004889"
                     /pseudo
     CDS             1..294
                     /locus_tag="POA77_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014609409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(564..1373)
                     /locus_tag="POA77_004890"
     CDS             complement(564..1373)
                     /locus_tag="POA77_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HDI6368193.1"
                     /translation="MTNMKLKFDLLLKSYHLSHRFVYKANPGNAGDGVIASATYDFFE
                     RNALTYIPYRDGECYSSETDILIFGGGGNLIEGLYSEGHDFIQNNIGKFHKVIIMPST
                     IRGYSDLFINNIDKFVVFCRENITFDYIKSLNYEPNKNVFITDDMAFYLDLNKYLSLK
                     PVYKKQANCFRTDSESLTGDYKENNHDISLTWNGDYWDNEFLARNSTRCMINFLEEYK
                     VVNTDRLHVAILASLLGKEVNFYPNSYYKNEAVYNYSLFNRYPKTCFITAS"
     gene            1471..>2335
                     /locus_tag="POA77_004891"
     CDS             1471..>2335
                     /locus_tag="POA77_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6368194.1"
                     /translation="MKKILIMTPDIEGPVRNGGIGTAFTALATTLAKKGYDVDVLYTC
                     GDYSESSVSKFSDWSRIYSTFGINLLRTGLIKEINIDAPYFRRKSYSIYLWLKENNIY
                     DTVISCEWQADLYYTLLSKKNGTDFENTKFIVNTHSSTLWADEGNYQLPYDQNHLELY
                     YMEKMVVEMADEVVSPSQYLIDWMLSKHWNVPEERHVILNCEPFQGFVTRDDVTVKIN
                     EKPASGVELVFFGRLETRKGLDIFLRALRKLSDEDKESISGVTFLGKNVTMGKTDSFT
                     YIMNQTKNLGLA"
BASE COUNT          740 a          408 c          487 g          700 t
ORIGIN      
        1 tctatattaa gcgacctgca aaactggggc attgaagaca tcctgatcgc ctgcgtgcgc
       61 gggctgaccg gcttcccgga ggcgataaac agcatttatc cgcagacaga ggttcagttg
      121 tgcgtcattc accaaatcag taactcgatt aaatacgtgg cctcaaatga ccataaagct
      181 tttatggctg atctgaaacc ggtatatcgc gcggggtcga aagaagccgc agaaacggtg
      241 ctggatgaac tggaagcgaa atgggatcag cagtatccag tgttgctcca gtcgtgacga
      301 cgcaagtggg aaaatctgtc agcatacaat agaatttaag aaacacggag tcagtaaaat
      361 actgtacgtg aaaagtgatg cggaaagtta aaacctcggt gcaacaacta aactcattga
      421 taagtttttt actgcgataa tcgatagatt tgcagactat aggcgtgcga atgaatagtc
      481 acctaaaagc tggcaggtaa taattaacaa tgctgtaaca gttatatggc tttcaggcag
      541 cgtattatta tacgctgcct ttttcaactt gccgtaataa agcatgtttt tggataacga
      601 ttaaaaagtg aataattgta aacagcttca tttttgtaat atgagttagg atagaagttg
      661 acttctttgc caagcagaga tgctaaaatt gccacatgca gcctgtcggt attgacaact
      721 ttatactctt caagaaagtt tatcatgcaa cgggtagaat tacgcgccag aaattcatta
      781 tcccaataat cgccattcca ggtgagcgaa atatcatgat tgttttcttt gtagtctcca
      841 gttagagatt cggagtccgt tctgaagcag ttggcctgtt ttttatagac gggtttaagt
      901 gacaggtatt tattaagatc gagataaaat gccatatcat cagtaatgaa tacgttcttg
      961 tttggttcgt agttgagaga tttaatataa tcgaaggtga tattttcgcg acaaaaaaca
     1021 acaaacttat caatattgtt gatgaataaa tcgctatacc ctctgattgt cgacggcatt
     1081 attattactt tatgaaattt cccaatatta ttctggataa agtcatgacc ttcagaatac
     1141 aatccttcta tcaggtttcc tccgcctcca aaaattaaaa tatcagtttc agaactgtag
     1201 cactcgccat ctctgtaagg gatataggta agagcatttc gttcaaaaaa gtcatacgtc
     1261 gcagatgcaa ttacaccatc tccagcatta ccagggtttg ccttatagac aaatcgatga
     1321 gatagatgat aagattttag aagcaaatca aattttaact tcatattggt cattttttat
     1381 cctgtttaac agaagctacc gactgctatc ttacactact tcaattcact aatatcatag
     1441 aaaagtctag gttacaaagg aagggttaca atgaagaaaa ttcttataat gacgccggac
     1501 attgaggggc ctgtccgtaa cggcggtatt ggtactgctt tcactgctct tgccactact
     1561 ttggcaaaaa aggggtatga tgttgatgta ttgtatacat gtggcgacta ttctgaatca
     1621 tctgtatcga aatttagcga ctggtcacgt atttatagta cctttggtat caatctgcta
     1681 agaaccggac tgataaaaga gattaatatt gatgcaccgt attttagaag gaaaagttat
     1741 tcaatttatc tctggttgaa agaaaataac atctatgaca ctgttatttc ttgtgagtgg
     1801 caggcagatc tttattacac tttattaagc aaaaagaatg gaacggattt tgaaaataca
     1861 aagttcattg taaatactca cagttcaacg ttatgggctg atgaaggtaa ttaccagctt
     1921 ccatatgatc agaaccatct tgaactctat tatatggaga aaatggtggt tgaaatggcg
     1981 gatgaagttg ttagtccgtc tcagtattta attgattgga tgttgagtaa gcactggaat
     2041 gttcctgaag aacgtcatgt aattttaaat tgcgagccat ttcaagggtt tgtgacgaga
     2101 gatgatgtta cagttaaaat aaatgaaaag ccagcttctg gcgttgagct tgtatttttc
     2161 ggccgccttg aaacccgtaa aggacttgac atattcctgc gtgcattaag aaaactatct
     2221 gatgaagata aagagagcat ttctggagta accttcctcg gaaaaaatgt caccatgggg
     2281 aaaactgatt catttactta tattatgaat cagactaaaa atttgggact cgcag
//
LOCUS       DAMZII010000288         2289 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMZII010000288 DAMZII010000000
VERSION     DAMZII010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.293"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            92..424
                     /locus_tag="POA77_004892"
     CDS             92..424
                     /locus_tag="POA77_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368195.1"
                     /translation="MESEQLITKITQTLKRPDGSEVRIVVEQAFGSGLTPSLGVYVLR
                     RPTTANNWQLCKTAPHKDWRTMSVDEYQKHGRSEMLRYVSIGEILRLSAAIGKPMSYV
                     DTCPGLQG"
     gene            481..744
                     /locus_tag="POA77_004893"
                     /pseudo
     CDS             481..744
                     /locus_tag="POA77_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            972..1778
                     /locus_tag="POA77_004894"
     CDS             972..1778
                     /locus_tag="POA77_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368196.1"
                     /translation="MANNYTQASFTILCSQEQAQMALDAIAYVTDTDVAEGEHLLSKP
                     VSECSLTELLVLGIIQNHPECDPFEPTFGQSESPEDNYELELKAEITGKGLAICHDES
                     INLDHAIAVTTAVLSVFNLPEMVTINAAFVCDRPRENEFGGATIVVTKDTHHYEEGFN
                     FSRLMNEAHDAGVQYALVKVNQYNHEYTYTQCYLMSCKKSESAYDVARHRLASDESTP
                     DNPGEDGVIILSEEDNTSMALHSVTELMPVVYESLSKLLPSLDELCPVTA"
     gene            1826..2272
                     /locus_tag="POA77_004895"
     CDS             1826..2272
                     /locus_tag="POA77_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368197.1"
                     /translation="MTNKILILSASCINEFASDVPQSVFFSIDTSLAKRIRELAAYVK
                     ENDLYAAEFFFYDCHWSETLEEDIEVLMSQAAFIESDSSGQETMLRDVMPSCRTETTS
                     IRVMKESFHLTAVPRHCGDDMMLNTPLIPLAELESNATVLITQQYS"
BASE COUNT          616 a          552 c          545 g          576 t
ORIGIN      
        1 atgaaaaaaa tcacttctta cttattagcc agagagccga ctttggtaaa atatccctac
       61 agcaacccaa ctgacggtcg aggttaatag tatggaatcc gaacagctca ttactaaaat
      121 tactcagaca ctgaaacgcc ccgacggctc tgaagtacgt attgtggtgg agcaggcatt
      181 cggcagtggc ctcacgcctt cgctgggtgt atatgtcctg cgtcgcccga ccactgccaa
      241 caactggcag ttgtgcaaaa ccgcaccgca caaggactgg cgcactatgt cggtggatga
      301 ataccagaaa cacgggcgtt cggaaatgct tcggtacgtc tccatcggcg aaatccttcg
      361 cctgagcgcc gcgatcggca aaccaatgag ctacgttgat acctgtccgg gtttacaggg
      421 ctgatgctgt tttttacacg tagcagaact gacaaattta aaaattgaag gcggagaaaa
      481 atgatgatta actaacaggg catcgctgta aataccccag acttaagccg actgtatatt
      541 atctcaggcc gcatgatgtt tgatgatgag gaagaggtga tgctggtcga agcggactcc
      601 tgcggtgaag ctgaagcggc attccagcaa catatcgacg aaggtgtagc gcacttggat
      661 caggtgcttg ttatttcgtc tgcgtcccta caatcactgt taccagacag gctcattgct
      721 tcctccctga cacataccgt ataaccaaat ctttcccgtc taataaattc cgcccttgtg
      781 gcggtttttt gttttcccgt atatttattt tacaccgtat ccagaataaa agttggatta
      841 ttaaatatgg cgctgtctgc tctataaaaa tttcgaacat atttcattat tggttattag
      901 gtgcaggtat ctgttttgat attatctatt cacagacagc aatcacattt aattaaataa
      961 ggagttatgc aatggcgaac aattacactc aggcatcctt cacaattctc tgttcacagg
     1021 aacaggcaca gatggcactg gacgcgattg cgtatgttac agacacggat gtggcggaag
     1081 gtgaacactt gctgtctaaa ccggtttcag agtgttccct cactgaattg cttgtcctgg
     1141 gtattattca gaaccacccg gagtgcgatc cgtttgagcc gacttttggt cagtcggagt
     1201 caccagaaga caattacgag ctggaactca aagcggaaat caccggtaaa ggcctggcaa
     1261 tctgccatga tgaaagcatt aacctcgacc acgctatcgc ggtaacaact gccgtgttgt
     1321 cggtgtttaa cctgcctgag atggtcacaa tcaatgccgc tttcgtctgc gaccgaccac
     1381 gcgaaaacga gttcggcggt gcgactattg tggtgactaa agatacacac cactacgaag
     1441 aaggctttaa tttttctcgc ctgatgaacg aagctcatga tgccggtgtt cagtatgcgt
     1501 tggtgaaagt gaaccagtac aaccacgaat acacatacac tcaatgctat ctgatgagct
     1561 gcaaaaaatc agaatcggct tatgacgttg cacgccaccg cctggcctct gacgagagca
     1621 cgcccgacaa tcctggtgag gatggagtta tcattctcag cgaagaagac aataccagca
     1681 tggcgcttca cagcgtcact gaattgatgc ccgtcgtgta cgaaagcctg agcaaattac
     1741 tgccttcact tgacgaattg tgccccgtga ctgcctgata atccctgatt gccaccggaa
     1801 cagccggtgg catggagagc tgaaaatgac caataaaatc ctgattttgt ccgctagctg
     1861 catcaatgaa tttgcgagtg atgtcccgca aagcgtcttc ttttccattg atacgtcgct
     1921 ggctaagcgt atccgtgaac tggcggcata cgtgaaagaa aatgatttgt acgccgccga
     1981 gtttttcttc tatgactgcc actggtccga aacattggaa gaggacattg aggttctcat
     2041 gtcgcaggct gcgtttattg aaagtgacag cagcggacag gaaacgatgc tccgggacgt
     2101 aatgccctcc tgccgcacgg aaacaacttc catccgggtc atgaaagaat catttcatct
     2161 gacggctgta ccccgtcatt gcggcgatga catgatgctc aatacgcccc taattccgtt
     2221 agcagagctg gaatcaaatg cgacggtttt aattacccag cagtattcgt gagagcgtgg
     2281 agaacataa
//
LOCUS       DAMZII010000289         2285 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMZII010000289 DAMZII010000000
VERSION     DAMZII010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.294"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            178..741
                     /locus_tag="POA77_004896"
     CDS             178..741
                     /locus_tag="POA77_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6368198.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            823..>2285
                     /gene="yfcU"
                     /locus_tag="POA77_004897"
     CDS             823..>2285
                     /gene="yfcU"
                     /locus_tag="POA77_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDI6368199.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMT"
BASE COUNT          616 a          512 c          578 g          579 t
ORIGIN      
        1 catcaggggg gtgaaaacat cactatgcgg ccccacacca aagaatacaa ttttctacga
       61 cctgttcttt ttggatcgca tgccgtattc aatggctggg tataaacatt gggtattgac
      121 tgaatcaatg tattgtaatg tttatatttt taattattta tagtttaagg acaaataatg
      181 agtaagtttg ttaaaacagc tattgctgcg gcaatggtga tgggcgtttt cacctctacg
      241 gcaacaatcg ctgcgggtaa caacggtact gcacgttttt acggcaccat tgaagactca
      301 gtgtgttcta tcgttccgga cgatcacaaa ctggaagttg atatgggtga tatcggtgcc
      361 gaaaaactga aaaataacgg caccaccacg ccgaaaagtt tccagattcg tctgcaagat
      421 tgcgtatttg atactcagga aacaatgacc actaccttta ctggtaccgt ttcttctgca
      481 aatagcggca attattacac cattttcaat accgataccg gtgcggcatt taacaatgtt
      541 agcctggcga tcggtgactc tctgggtacc tcttacaaaa gcggcatggg tattgaccag
      601 aaaatagtga aagacacttc taccaataaa ggcaaagcga agcagacact gaactttaaa
      661 gcctggctgg tcggcgcagc tgatgcgcca gatctgggta atttcgaagc aaataccacc
      721 ttccagatta cttacctgta aggcataccc ccatagcgac actgctatgg gggatttaaa
      781 tggattttga ccgaacaaga ataaaaacgg atagaaacgt gtatgcctga ccattctctt
      841 tttcgattac ggattcttcc gtggtgcatt gcgctggcaa tgtcagggag ttatagcagt
      901 gtctgggctg aagacgacat tcagtttgat tcccgttttc tggaattaaa aggcgacaca
      961 aaaattgatc tgaagcgttt ttccagccag ggatatgttg agcccggaaa atacaattta
     1021 caggttcaac taaataaaca gccattggcg gaagagtacg atatttactg gtatgctggt
     1081 gaagatgacg cgagcaaaag ctatgcttgt ctgacaccgg aactggtagc gcagtttggt
     1141 ttaaaagaag acgtggcgaa aaatctgcaa tggagccacg atgctaaatg cctgaaatcc
     1201 ggtcaactgg aaggcatgga aattaaggct gatttaagcc agtccgcatt agtcatttca
     1261 ctgccacagg cttacctcga atatacttac cccgactggg atccgccttc acgttgggat
     1321 gacggcatct ccgggatcgt cgcggactac agcatcaacg cacaaacccg gcacgaagaa
     1381 aatggcggtg atgatagtaa cgagatcagc ggcaacggga cggtcggggt taacctgggg
     1441 ccgtggcgta tgcgtgctga ctggcagact aactatcaac atactcgcag taatgatgac
     1501 gatgaattca gcggcgatga aactcaaaaa aaatgggagt ggagtcgcta ctatgcctgg
     1561 cgggcgttac catcattaaa agccaaactg gcgctgggcg aggattacct cagatccgat
     1621 atttttgatg gttttaacta tgttggtggc agtgtcagta ctgacgatca aatgttgcct
     1681 cccaatctgc gcggctacgc gccagacatt tccggcgtgg cacacaccac agcaaaagtg
     1741 accgtcagcc agatggggcg tgtgatttac gaaacgcagg ttccggctgg accgtttcgt
     1801 attcaggatc ttggtgattc tgtctccggt acgttgcata ttcgcattga agaacagaac
     1861 ggccaggtgc aggaatatga catcagcacc gcctcgatgc catacctcac tcgcccaggt
     1921 caggttcgct ataagatcat gatgggccgt ccgcaagagt ggggacacca tgtcgagggt
     1981 gaattttttt ctggtgctga agcttcctgg gggatcgcta acggctggtc gttatatggc
     2041 ggcgcactgg gagatgaaaa ctatcagtct gcggcgcttg gcgtcggtcg cgatttgtct
     2101 acattcggcg cggtcgcgtt tgatgttact cactcgcaca ccaaactgga taaagacacc
     2161 gcttatggca aaggttcgct ggacggtaac tccttccgtg tgagttattc caaagacttt
     2221 gaccagctca acagccgcgt tacttttgct ggatatcgct tctcggaaga gaactttatg
     2281 accat
//
LOCUS       DAMZII010000290         2278 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMZII010000290 DAMZII010000000
VERSION     DAMZII010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.295"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(221..928)
                     /locus_tag="POA77_004898"
     CDS             complement(221..928)
                     /locus_tag="POA77_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368200.1"
                     /translation="MKHSLFFTQDGYLLPSSVKPIYGKDGHYYAVITRECFECGGTGK
                     IANAVCVTCNGSKTSPATEEKLVLKECIEFLETIPELRCNPDLEAWKRRHSEQLALLQ
                     ADNSKFAASIAQQLNNNLFLTVRQLESFYSTYQPVSEAFSENAGFPIEHSFNIGDVVS
                     FDIIFTEVRAAFSKGSHSYFFTFTGKKQNDGFALTGNVQDHVLLGHSYHLSGKVRKIH
                     YFDSVPYALLTVFKLTA"
     gene            complement(925..1125)
                     /locus_tag="POA77_004899"
     CDS             complement(925..1125)
                     /locus_tag="POA77_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368201.1"
                     /translation="MDKIAQFTVINTYNDLLITKGTLDRNIEEIKAGKRFDHFEEGHD
                     QYVQKVTDALQLVEARLENLKK"
     gene            complement(1143..2219)
                     /locus_tag="POA77_004900"
     CDS             complement(1143..2219)
                     /locus_tag="POA77_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368202.1"
                     /translation="MNHCDYQIIEISPKALFIESGFNPREAVIGELCYEQEPVKSTIV
                     SIKQAYKEGRRVDLIKVVKKDSNYVVRQGHCRYRALSLALAEGADIQLISVVVMNYKN
                     KSEEYLENLDGNRNNGLNPVALAYALAEALKLGSTVESLAMRYQRSSTAIRNQLKILD
                     MPLELQRLISLNLIKKTLAIEIMLKYSNDHAKVLAHLESCSLLVPSTEPEPVLSKTSS
                     VQTSSNDEKLDNPETLPVHVIPKEVKTKSITRSSLGIKRLSHKKTERLKTEFIGLIDT
                     VRPALNDSNKLTELIFDGQHSNLFTNISSTSITKTTESVKITLDEAKLQLLLSLLNDE
                     NTAIIIPKSKVTELHEIRDLLTSE"
BASE COUNT          663 a          442 c          464 g          709 t
ORIGIN      
        1 aaatataatt aataagatta tcatttttaa tttaaaagat tagttaataa cgggtgttga
       61 atttaaaata aatcggctta ttttcggagt gtaaaaccgg gtaaaaaccc gatgcgtgag
      121 caacagattt gacgtgagtg agtcggcacg acagtgcaga aagccggtgg ctaatgtaaa
      181 aacggcctgt atttgggagt tccggtcact agacaccagg ctatgctgtt aatttaaaca
      241 cagtcagtaa tgcgtagggc actgagtcaa agtagtggat ctttctcact tttcccgaaa
      301 gatgatagct atgccccaat aaaacgtgat cctgaacatt accagtcaac gcgaatccat
      361 cattctgctt tttgcctgta aaagtaaaaa aatagctatg agaacctttt gaaaacgcag
      421 ctcgcacttc tgtaaaaata atatcaaaac taactacatc accaatgttg aaagaatgtt
      481 caattggaaa accggcgttt tctgaaaaag cttcagaaac aggctggtat gtcgagtaga
      541 aagactccaa ctgacggact gttaaaaaga gattgttgtt taactgctgt gcaatgcttg
      601 ctgcaaactt agaattgtca gcttgcagca atgctaactg ctcgctgtgc ctacgtttcc
      661 aggcttcaag atcgggattg cagcgaagtt ccgggatagt ttcaaggaat tcaatgcact
      721 ctttcagtac cagcttttcc tctgtggctg gagacgtttt ggagccattg catgtcacac
      781 agacggcgtt cgcaatcttt ccagtccctc cgcattcaaa gcactcacgg gtgataacgg
      841 cgtaatagtg tccatcttta ccgtagatag gctttacgga tgatggcagt aaataaccat
      901 cctgagtaaa aaaaagagaa tgtttcattt tttaaggttc tccagtcgag cctccacgag
      961 ctgtaaagcg tcagttacct tctgtacgta ttgatcgtgc ccttcttcaa aatgatcaaa
     1021 acgctttcca gccttgatct cttcaatgtt gcgatcgagt gtgccttttg taataagtag
     1081 atcgttataa gtattaatta cagtaaactg tgcaatcttg tccataaacg gtccttagca
     1141 ttctactcac ttgttaacag gtcacgaatt tcatgcagtt ctgtaacctt agattttgga
     1201 atgatgatag cagtattttc atcattcaga aggcttaata aaagctggag ttttgcttca
     1261 tctagagtga tctttacact ttccgttgtc tttgtgatgg aagtgctgct gatgttcgta
     1321 aataaattgg aatgttgtcc gtcgaaaatt aattcggtga gtttattact gtcgtttagt
     1381 gctggccgta cagtatcaat cagcccgata aattcagttt ttaagcgttc agttttttta
     1441 tgagaaagcc gtttaatacc caatgaactg cgagtgatgg attttgtttt aacttcctta
     1501 ggaataacat gaacagggag tgtttccggg ttatctagtt tctcatcgtt tgatgatgtt
     1561 tgaacgcttg atgtcttaga taaaacaggt tctggttcag ttgatggtac taaaagagaa
     1621 cacgattcca aatgtgcaag gacttttgca tggtcattgg aatacttcaa catgatctca
     1681 atcgcgagtg tttttttaat caggtttaga ctgattaaac gttgtagttc taaaggcata
     1741 tcaaggattt taagctggtt acgaatagca gtgctagacc gttgatagcg catagcaagg
     1801 gattcaacgg tagagccaag cttcagcgcc tctgccagtg cataagctaa ggctacggga
     1861 ttaagaccgt tattcctatt cccatcaagg ttctccaggt attcttcact tttattctta
     1921 tagttcataa caactactga aatgagctga atatcagctc cttccgcaag agcaagagat
     1981 agtgctctgt aacggcaatg cccctgcctt acaacgtaat tggagtcttt cttaacaacc
     2041 ttaattaaat cgactcgtct gccctcttta taggcttgtt taatagaaac aatggtcgat
     2101 tttactggtt cctgttcata acaaagttca ccaattaccg cttcacgtgg attgaatcca
     2161 gattcgataa aaagtgcttt aggactgatc tcaataatct gatagtcgca gtgattcata
     2221 taacctcctt tttattacat gatacattaa ttgactcagt ttcaaataac ccaaaagc
//
LOCUS       DAMZII010000291         2225 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMZII010000291 DAMZII010000000
VERSION     DAMZII010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.296"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..271)
                     /locus_tag="POA77_004901"
                     /pseudo
     CDS             complement(1..271)
                     /locus_tag="POA77_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            360..1382
                     /gene="istA"
                     /locus_tag="POA77_004902"
     CDS             360..1382
                     /gene="istA"
                     /locus_tag="POA77_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDI6368203.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1382..2161
                     /gene="istB"
                     /locus_tag="POA77_004903"
     CDS             1382..2161
                     /gene="istB"
                     /locus_tag="POA77_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDI6368204.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDETGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          566 a          593 c          568 g          498 t
ORIGIN      
        1 acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct
       61 gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc
      121 agccatccgt catccatatc accacgtcaa agggtgacag caggctcata agacgcccca
      181 gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat
      241 acgcgtaaaa cagccagcgc tggcgcgatt tagtgtcacg aacggtgcaa tagtgatcca
      301 cacccaacgc ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta
      361 tggtcacttt tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc
      421 gggcgattgc cagagaactg gggatctccc gcaatacggt taaacgttat ttgcaggcaa
      481 aatctgagcc gccaaaatat acgccgcgac ctgctgttgc ttcactcctg gatgaatacc
      541 gggattatat tcgtcaacgc atcgccgatg ctcatcctta caaaatcccg gcaacggtaa
      601 tcgctcgcga gatcagagac cagggatatc gtggcggaat gaccattctc aggggattca
      661 ttcgttctct ctcggttcct caggagcagg agcctgccgt tcggttcgaa actgaacccg
      721 gacgacagat gcaggttgac tggggcacta tgcgtaatgg ccgctcaccg cttcacgtgt
      781 tcgttgctgt tctcggatac agccgaatgt tgtacatcga attcactgac aatatgcgtt
      841 atgacacgct ggagacctgc catcgtaatg cgttccgctt ctttggtggt gtgccgcgcg
      901 aagtgttgta tgacaatatg aaaactgtgg ttctgcaacg tgacgcatat cagaccggtc
      961 agcaccggtt ccatccttcg ctgtggcagt tcggcaagga gatgggcttc tctccccgac
     1021 tgtgtcgccc cttcagggca cagactaaag gtaaggtgga acggatggtg cagtacaccc
     1081 gtaacagttt ttacatccca ctaatgactc gcctgcgccc gatggggatc actgtcgatg
     1141 ttgaaacagc caaccgccac ggtctgcgct ggctgcacga tgtcgctaac caacgaaagc
     1201 atgaaacaat ccaggcccgt ccctgcgatc gctggctcga agagcagcag tccatgctgg
     1261 cactgcctcc ggagaaaaaa gagtatgacg tgcatcctgg tgaaaatctg gtgaacttcg
     1321 acaaacaccc cctgcatcat ccactctcca tctacgactc attctgcaga ggagtggcgt
     1381 gatgatggaa ctgcaacatc aacgactgat ggcgctcgcc gggcagttgc aactggaaag
     1441 ccttataagc gctgcgcctg cgctgtcaca acaggcagta gaccaggaat ggagttatat
     1501 ggacttcctg gagcatctgc ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc
     1561 gatgtatacc cgaatggcag ccttcccggc ggtgaaaacg ttcgaagagt atgacttcac
     1621 attcgccacc ggagcaccgc agaagcaact ccagtcgtta cgctcactca gcttcataga
     1681 acgtaatgaa aatatcgtat tactggggcc atcaggtgtg gggaaaaccc atctggcaat
     1741 agcgatgggc tatgaagccg tccgtgcagg tatcaaagtt cgcttcacaa cagcagcaga
     1801 tctgttactt cagttatcta cggcacaacg tcagggccgt tataaaacga cgcttcagcg
     1861 tggagtaatg gccccccgcc tgctcatcat tgatgaaaca ggctatctgc cgttcagtca
     1921 ggaagaagca aaactgttct tccaggtcat tgctaaacgt tacgaaaaga gcgcaatgat
     1981 cctgacatcc aatctgccgt tcgggcagtg ggatcaaacg ttcgccggtg atgcagcact
     2041 gacctcagcg atgctggacc gtatcttaca ccactcacat gtcgttcaaa tcaaaggaga
     2101 aagctatcga ctcagacaga aacgaaaagc cggggttata gcagaagcta atcctgagta
     2161 aaacggtgga tcaatattgg gccgttggtg gagatataag tggatcactt ttcatccgtc
     2221 gttga
//
LOCUS       DAMZII010000292         2160 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMZII010000292 DAMZII010000000
VERSION     DAMZII010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.297"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>2160
                     /gene="rhsB"
                     /locus_tag="POA77_004904"
     CDS             <1..>2160
                     /gene="rhsB"
                     /locus_tag="POA77_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDI6368205.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT
                     GPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRY
                     GQLLSFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLTAVKDTQGHETRY
                     EYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTV
                     KGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQ
                     HETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVDFTRDRLHRETLR
                     SFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGR
                     LTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRH
                     GRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRR
                     VAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQ"
BASE COUNT          515 a          669 c          657 g          319 t
ORIGIN      
        1 cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac ggatgccgca
      361 ggccggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 actgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc
      601 tgcgcaacgg aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggccag
      661 ttgctgagct tcaccgactg ttccggctac cagacccgtt atgaccatga ccgcttcggg
      721 cagatgacgg cggtgcaccg cgaggaaggg ctgagccagt accgcgcata cgacagccgt
      781 ggacagttaa ctgcggtgaa agacacgcag ggccatgaaa cgcggtatga atacaacatc
      841 gccggtgacc tgaccgccgt cattgccccg gacggcagca gaaacgggac acagtacgat
      901 gcgtggggaa aggccgtccg taccacgcag ggcgggctga cgcgcagtat ggaatacgat
      961 gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac
     1021 gatgtacttg accggctgat acaggaaacc ggctttgacg gccgcacaca gcgttatcac
     1081 cacgacctga ccggcaaact tatccgcagc gaggatgagg gtctggtcac ccactggcac
     1141 tatgacgaag cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc agagcggtgg
     1201 cggtacgacg aacgcggctg gctgacagac atcagccata tcagcgaagg gcaccgggtg
     1261 acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat
     1321 cacccgcaga cggaagcact gctctggcag catgagacca gacatgcgta caacgcgcag
     1381 gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
     1441 agcggttacc tggcaggcat gaaactcggc gacacaccgc tggtggattt cacccgcgac
     1501 cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg aactcaccac cgcttacacc
     1561 cctgccgggc agttacagag ccagcacctg aacagcctgc tgtctgaccg cgattacacc
     1621 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
     1681 tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaatct ggatatccgc
     1741 atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac
     1801 agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta tctttaccgg
     1861 tatgaccgtc acggcaggct gacagagaaa accgacctca tcccggaagg ggttatccgc
     1921 acggatgatg agcggactca ccggtaccat tacgacagtc agcaccggct ggtgcactac
     1981 acgcggacac aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc
     2041 cgcagggtgg caaaacgggt gtggcggcgt gaacgggacc tgacgggctg gatgtcgctg
     2101 tcacggaaac cgcaagtgac ctggtacggc tgggacggcg accggctgac cacaatacag
//
LOCUS       DAMZII010000293         2143 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMZII010000293 DAMZII010000000
VERSION     DAMZII010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.298"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..421
                     /locus_tag="POA77_004905"
     CDS             <1..421
                     /locus_tag="POA77_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6368206.1"
                     /translation="KGENLALLHGLTPDILDRIYAYAFDYHEKGNITDAEIYYKFLCI
                     YAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPYDDYSVIYRMGQCQIGAKNID
                     NAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
     gene            756..2132
                     /gene="ygeH"
                     /locus_tag="POA77_004906"
     CDS             756..2132
                     /gene="ygeH"
                     /locus_tag="POA77_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDI6368207.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVRGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYVSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          777 a          358 c          360 g          648 t
ORIGIN      
        1 gaaaggagaa aatctggcat tactacatgg tttaactcct gatatccttg atagaatata
       61 tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa tttattataa
      121 atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaagattttg catcagtatg
      181 tcagcccaaa aagaaatatc aacaagcata tgacctttac aaactaagtt acaattactc
      241 cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg gggctaaaaa
      301 tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg atgatagtgt
      361 taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag ataatggcta
      421 agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaaccac tataaccagc
      481 acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact ataaatcatt
      541 acttttcaat gcgttagaga acaatctact ttataaaaat tgaataaggc tatttcagat
      601 tacatcactg aattcctaag ataaattagt gaacacaacc atagatatgt cgaatgtaat
      661 ttcctgtagc attaatgaca cataaatatg aatagccata atttctattg cttaagcagg
      721 atgcaagaaa ccaatttttt catagaggtt aactaatgga cttagaaaat aaattctcat
      781 atcattttct tgagggatta acgctcacgg aagatggaat tcttactcaa ggaaatgagc
      841 aagtttatat tccacagaaa gagttaggtg tattaatagt attacttgaa tccgctggtc
      901 atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt gttagtgacg
      961 agtccctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt ggctatgatc
     1021 gttgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt ttcaaaacta
     1081 aaattaatga agataatact tcagactatt ccatagctat attccctttc actacttcat
     1141 tgaatacact ggatccatta atacttaatc aggaattagt gcaaatcatt tcaaataaaa
     1201 aaatcgatgg tctctatacc tatccgatgg ctgcgacaaa tttttgtaat gatcacatat
     1261 ctcaaaattc attcttgagc agattcaagc cagattattt cgttagagga agaataaacc
     1321 agaataatgc agtgaacact ttatacattg agttgatcga cgctaaaaac cttttcctca
     1381 tcgccagtaa tcatctccct gttgatgaac tacataatac atcacaattt attatagata
     1441 atatccttca aacggtacat aaaccagaac gatctgtaag attagctaag caggaccaag
     1501 gatataagaa tcattatgta tcagatgaaa tgttagccgg aaagaaagaa ctttacgact
     1561 tcacccctga aagcatttac agggccatga ctatatttga tagattacaa aataaaagtg
     1621 atatacagac gctaaaaaca gaatgttatt gccttctagc ggaatgccat atgtctttgg
     1681 cacttcatgg aaaaagtgaa cttgaacttg ctgctcaaaa agcattagag cttttagatt
     1741 atgtatcaga cataaccact gtcgatggaa aaattttagc tattatggga ctgataactg
     1801 gtctgtctgg acaagcaaaa gtatctcata tcttatttga acaggctaag atacactcaa
     1861 ctgatatagc ctctctctac tactataggg cactagtcca ctttcataat gaaaaaattg
     1921 aagaggcaag gatttgtata gacaaatcac tacaactcga acccagaaga cgaaaagcag
     1981 ttgtgataaa agaatgtgta gatatgtatg tacctaaccc gctcaaaaac aacattaaac
     2041 tctactataa agaaactgag agtgaaagcc atcgagttat aattgacaac attttgaaat
     2101 taaagcagct gacgagaatt tgtatgcgat aaatttttat tga
//
LOCUS       DAMZII010000294         2083 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMZII010000294 DAMZII010000000
VERSION     DAMZII010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.299"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1400
                     /locus_tag="POA77_004907"
     CDS             <1..1400
                     /locus_tag="POA77_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6368208.1"
                     /translation="YLYRYDRHGRLTKKTDLIPEGGIRTDDERTHRYHYDSQHRLVHY
                     TRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTT
                     IQNDRSRIQTIYQPGSFTPLIRVETATGELARTQRRSLADTLQQSGGEDGGSVVFPPV
                     LVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRG
                     LPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYY
                     DPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPT
                     PDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQ
                     DDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGE
                     ILAKMKQRTSRGIRK"
     gene            1397..1966
                     /locus_tag="POA77_004908"
     CDS             1397..1966
                     /locus_tag="POA77_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368209.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRFNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          614 a          468 c          533 g          468 t
ORIGIN      
        1 actatcttta ccggtatgac cgtcacggca ggctgacgaa gaaaaccgac ctcatcccgg
       61 aagggggtat ccgcacggat gatgagcgca cccaccggta ccattacgac agtcagcacc
      121 ggctggtgca ctacacgcgg acacaatatg cagagccgct ggtcgaaagt cgctatcttt
      181 acgacccgct gggccgcagg gtggcaaaac gggtatggcg acgtgaacgg gacctgacgg
      241 gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac ggcgaccggc
      301 tgaccacgat acagaacgac aggagccgca tccagacgat ttatcagccg gggagcttca
      361 caccactcat cagagttgaa actgccaccg gtgaactggc cagaacgcag cgccgcagcc
      421 tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg
      481 tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg
      541 aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatacaa aaccagatgg
      601 acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc
      661 cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac gatgaatggg
      721 gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc ctgccggggc
      781 agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat gacccgctgc
      841 aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat ttttatcagt
      901 atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa tatggacact
      961 taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat ccaagtaaat
     1021 tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc agtagtgcgc
     1081 ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca ctgtattgca
     1141 ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag tatccaggga
     1201 tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca actcaattac
     1261 caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa cccgatgggc
     1321 cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa cagagaacat
     1381 cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat ttgttagttt
     1441 tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta ctattttcgc
     1501 taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt ggagatggta
     1561 tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat gtaaaaataa
     1621 taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac gctttaatga
     1681 aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa aaacggcgtt
     1741 tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa agagcagtga
     1801 tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt ttaaagatga
     1861 gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt cttacgaatg
     1921 tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc tcttcctgag
     1981 aaaacaaatt catcattgtg aaaaattata taactcatgg aataggtgtt tattttgcgt
     2041 tccatttgca gggaaagatc acgtaacgct acttttttgt act
//
LOCUS       DAMZII010000295         2071 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMZII010000295 DAMZII010000000
VERSION     DAMZII010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.300"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            25..>2071
                     /locus_tag="POA77_004909"
     CDS             25..>2071
                     /locus_tag="POA77_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6368210.1"
                     /translation="MAEKTTLFVGANNSGKTAAMEALGHFLVDASRFSVNDLTLCNWA
                     KIKDITEQWLTEENIDLAKSQEAFSDLFPCMDIWIEAKENELQYVNHILPTLEWNGGL
                     IGVRLRYEPKKFDVFFEDFISSRQNSLDIHDRISERIDKENYTIALWPKDIYEYLSRK
                     LNSMFTIKCYSLDPSKLESSDNGIAKPQRIDSQQTLLDGIPLKDLIRIDEINAQRGFG
                     SPSAADKENSKLNTQLRDYYSKHLNPIESPELSDIEALQAIRDAQDTFDERLKNCFSQ
                     AIKEVEGLNYPGVTDPKLKISTKIEPIDGLNHDSAVQYELTNALSNVSLDYLRLPESY
                     NGLGYQNLISIAFRLMGFRDSWMKVGKATNKKLADDNQEDFPAPLHLVLIEEPEAHLH
                     AQVQQIFIRKAYDILRNHNNLGDKEELHTQLIISTHSSHIAHECKFSSLRYFRRIPAC
                     KEHEIPVSTVINLSNVFGVNDDTEKFVIRYLKATHCDLFFADAVILVEGPAERILVPH
                     LIRRHYPFLNQSYITLLEIGGSHAHRLKNLIVSLNLLTLIITDLDPKDSQDGKVKPSK
                     KGSGFVTSNTTLASWVPEVTGVDELYKLTEDKKEKKYNDFFYVYSVFQIPVLAAFKGV
                     NQEFLPNTFEDSLVYNNLDFFSNLTGNGLVKKFKASITNCKNAEEFGEESFLHLSKGN
                     "
BASE COUNT          724 a          359 c          380 g          608 t
ORIGIN      
        1 aagttgaagt ctgtccgcat agatattgca gaaaaaacca ctctctttgt tggtgctaac
       61 aatagcggta aaacggctgc aatggaggcc ttaggacatt tcctcgttga tgcctcgcgg
      121 tttagcgtaa atgatttgac actttgcaat tgggcaaaaa taaaagatat cactgaacaa
      181 tggttaactg aggaaaatat agaccttgct aaatcacagg aagctttctc agatcttttt
      241 ccttgcatgg atatttggat cgaagcgaaa gaaaatgagc tccaatatgt caatcatata
      301 ctaccaacgt tagagtggaa tggaggttta attggagtaa gactgagata tgaacccaag
      361 aaatttgatg ttttctttga ggacttcata tcttcccgcc aaaattcatt agatatacac
      421 gaccgaattt ctgagcggat tgataaagaa aattacacaa tagcattgtg gcctaaagat
      481 atttatgaat atctttcaag aaagttgaac tcaatgttta caattaagtg ttatagctta
      541 gatccttcga aattagaatc atctgataat ggaatagcca aaccccaaag aatagacagt
      601 cagcaaacat tacttgatgg catacctctc aaagatctaa taagaattga tgagatcaat
      661 gcacaacgtg gctttggaag cccctctgct gccgacaaag agaatagcaa attaaacaca
      721 cagttgagag attattattc gaaacatctt aacccaatag aaagccctga actatcagat
      781 atcgaagctc tacaagccat tcgtgatgct caggatacat ttgatgaaag attaaaaaac
      841 tgtttctccc aagccataaa agaagttgaa ggcctcaact atccaggagt aactgatcct
      901 aaactaaaaa tctctacaaa aatagaacca attgatggct taaatcacga ctcagcagtt
      961 caatatgagt taactaatgc tttaagcaat gtatctttag actatttaag attgccagag
     1021 agttataatg gactaggtta tcaaaactta atctcaattg catttagact aatggggttc
     1081 agagatagtt ggatgaaagt tggcaaagct acaaataaaa aattagcaga cgacaatcaa
     1141 gaggattttc cagctcccct gcatttggtt ttgattgaag aacctgaagc tcacttgcat
     1201 gcacaagtgc aacaaatctt cattaggaaa gcatatgata tattgaggaa tcataataat
     1261 ttaggcgata aagaagagct tcatacacaa ctcattataa gcacacattc gagccatatt
     1321 gcccacgagt gtaaattttc ttctttacga tactttagaa gaattcctgc ttgtaaagaa
     1381 catgagatcc ctgtatcaac agtaatcaat ctttccaatg tttttggtgt aaatgatgat
     1441 acggaaaaat ttgtaataag atacctaaag gcaacacatt gtgatttatt ctttgcagat
     1501 gcagtaattc ttgttgaagg cccagccgag aggattttag ttcctcactt aattagaaga
     1561 cactaccctt tcttaaatca aagctacatt accttattag agattggtgg tagccatgcc
     1621 catcgactaa agaatctgat tgttagtttg aatcttttaa ctctaattat tacggattta
     1681 gaccctaagg attcacaaga tggcaaggtt aagccctcta agaaaggcag cggtttcgtt
     1741 acaagtaata caacgcttgc aagttgggtt ccagaagtta ccggtgtcga tgaattgtat
     1801 aaactcacgg aagataagaa agaaaagaaa tataatgatt tcttttatgt ttactcagtt
     1861 tttcagattc ctgttttggc tgcattcaaa ggtgttaacc aagaattttt accaaacacg
     1921 ttcgaagact cattagtcta caataatcta gattttttca gtaatcttac tggtaatggt
     1981 ttagtgaaga aattcaaagc atcaataact aattgcaaaa atgcagagga atttggagag
     2041 gaatcatttt tacacttatc taaaggtaat a
//
LOCUS       DAMZII010000296         1892 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMZII010000296 DAMZII010000000
VERSION     DAMZII010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.301"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..448)
                     /locus_tag="POA77_004910"
     CDS             complement(<1..448)
                     /locus_tag="POA77_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HDI6368211.1"
                     /translation="MPRLTAPRRQAEVCAPLPAAHAAARYARHLPERTLLYALVQAHY
                     PDFIARLEAEDRPLPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEA"
     gene            complement(637..>1892)
                     /gene="groL"
                     /locus_tag="POA77_004911"
     CDS             complement(637..>1892)
                     /gene="groL"
                     /locus_tag="POA77_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6368212.1"
                     /translation="EELKKISKPCSTSKEIAQVGSISANSDTDIGELIAKAMDKVGKE
                     GVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDPFILLHDKKISNV
                     RDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAVKAPGFGDRRKAM
                     LEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIIDGAGDTADIEARI
                     KQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATR
                     AAVEEGIVPGGGVALIRAKAAIAELKGANEDQNHGIAIALRAMEAPLREIVTNAGDEP
                     SVVLNRVAEGTGAFGYNAANGEFGDMIEFGILDPTKVTRSALQNAASIAGLMITTEAM
                     VAEAPKKEEPAAPGGGMGGMGGMDF"
BASE COUNT          259 a          653 c          630 g          350 t
ORIGIN      
        1 tggcctccgg cttgctggcg aacaggaagc gcagcgggta cggaaaactc agcacccatt
       61 gccgcaccgg ccgcgggcca aacacctcct ccaccaggtg ccgcgcgctc tcggccatgc
      121 gccgcgcccc gcagctgggg cagaagccgc gcttcttgca ggagaaggcc accagcctct
      181 cggcccggca ctgctcgcac accacgcgca ggaaaccgtg ctcgagcacg ccgcaacgca
      241 ggtaggtctc gaactcctcg cgcacatact cgggcagcgg gcggtcttcg gcctcaagac
      301 gcgcgatgaa gtccgggtag tgcgcctgca ctagcgcgta cagcagcgtg cgctcgggca
      361 ggtggcgcgc gtaacgcgca gcggcgtggg cggccggcag tggcgcgcac acctcggcct
      421 gccgccgggg tgcggtcagg cgcggcacgc agcgctccgt tgcagggacg gctgctcagg
      481 gttgcgcccg cgagcacgcg ttcatatcgg cgttttctga tcttcgcgtc agacattgcc
      541 gcggggcggg taggaagtct cccgcctcgg acgccagaac gcacaaagcc cggcacgagg
      601 ccgggctttg tggtcttgct tgatggatcg cggggcttag aaatccatgc cgcccatgcc
      661 gcccatgccg ccgcccggag cggccggctc ttccttcttc ggcgcttcgg ccaccatcgc
      721 ttcggtggtg atcatcaggc cggcgatgga cgccgcgttc tgcagcgcgg agcgcgtgac
      781 cttggtcggg tccaggatgc cgaactcgat catgtcgccg aactcgccgt tggcggcgtt
      841 gtagccgaac gcgccggtgc cttcggcgac gcggttcagc accacgctcg gctcgtcgcc
      901 ggcgttggtg acgatctcgc gcagcggggc ttccatcgcg cgcagggcga tcgcgatgcc
      961 gtggttctgg tcctcgttgg cgcccttcag ctcggcgatc gcggccttgg cacggatcag
     1021 ggcgacgccg ccgcccggga cgatgccttc ctcgaccgcc gcacgggtgg catgcagggc
     1081 gtcttcgacg cgcgccttct tttccttcat ctcgacttcg gtggcggcgc cgaccttgat
     1141 caccgcaacg cccccggcca gcttggccac gcgctcctgc agcttctcgc ggtcgtagtc
     1201 cgaggtggtc tcctcgatct gcgccttgat ctgcttgatg cgggcttcga tgtccgcggt
     1261 gtcgccggcg ccgtcgatga tggtggtgtt ctccttcgag acctgcacct tcttcgcgcg
     1321 gccgaggtcg ttgatggtcg ccttctcgag cgagaggccg acttcctcgg agatcacggt
     1381 gccaccggtg aggatggcca tgtcctccag catcgccttg cggcggtcgc cgaagcccgg
     1441 ggccttgacc gcgcagacct tcacgatgcc gcggatggtg ttgaccacca gcgtggccag
     1501 cgcctcgccc tcgacgtcct cggcgacgat cagcagcggc ttgccggcct tggccacgcc
     1561 ctcgaggatc ggcagcaggt cgcggacgtt cgagatcttc ttgtcgtgca gcaggatgaa
     1621 cggatcctcc agctcggcct gcatcgactg cgggttgttg atgaagtacg gcgacaggta
     1681 gccgcggtcg aactgcatgc cctcgacgac gtcgagctcg ttctccaggc ccgagccctc
     1741 ctcgacggtg atcacgcctt ccttgccgac cttgtccatg gccttggcga tcagctcgcc
     1801 gatgtcggtg tcggagttgg ccgagatcga accgacctgg gcgatctcct tgctggtcga
     1861 gcagggcttg gagatcttct tcagctcctc ga
//
LOCUS       DAMZII010000297         1854 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMZII010000297 DAMZII010000000
VERSION     DAMZII010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.303"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            29..400
                     /locus_tag="POA77_004912"
     CDS             29..400
                     /locus_tag="POA77_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDI6368213.1"
                     /translation="MNKKINAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKITN
                     FADWITEFGKAARTDNWRPFFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLM
                     AVTGALIDETLVEKANKLWGI"
     gene            complement(422..757)
                     /locus_tag="POA77_004913"
     CDS             complement(422..757)
                     /locus_tag="POA77_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDI6368214.1"
                     /translation="MNRKYYLNNMWWGWFMGGYTLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVARWCIENTALKFTRPHFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYILS
                     VIIKRLSVR"
     gene            886..1233
                     /locus_tag="POA77_004914"
     CDS             886..1233
                     /locus_tag="POA77_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368215.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1253..1843)
                     /locus_tag="POA77_004915"
     CDS             complement(1253..1843)
                     /locus_tag="POA77_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368216.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
BASE COUNT          452 a          486 c          467 g          449 t
ORIGIN      
        1 aaaaacgtat gaaaagtacc gggctgacat taacaaaaaa attaatgcaa aagatcgtgc
       61 agcgattgcc gcagcccttg agtctgtgaa gctgtctgat atatcgtcta atctgaacag
      121 gttcagtcgc ggactgggat atgcaggaaa aattacaaat tttgctgact ggatcactga
      181 gtttggtaag gctgcccgga cagataactg gcgtcctttt tttgttaaaa cagaaaccat
      241 catagcaggt aatgccgcaa cggctcttgt tgctctggtc ttcagtattc ttaccggaag
      301 cgcgttaggc attatcgggt atggtttact gatggctgtc accggtgctc tgattgatga
      361 aacgcttgtg gaaaaagcga ataagctctg gggcatttaa ataatatata agacaggctg
      421 tctatcttac agacagcctt tttatgataa cagaaagaat atatatcaga gttaacggaa
      481 gagataatat gaaaacagta cccgtataaa ccgcaagtaa tcccattttt ccgggagtat
      541 cagcaaaaaa accgctgttc cagaaatgag gccgggtaaa tttcagggca gtattttcaa
      601 tacaccatct ggcaaccgga tacaaaacca ttccgcagag agaaatacac cagaacagca
      661 atctgtattt aaactcataa tcccatgaca tatacagagt ataccccccc ataaaccatc
      721 cccaccacat attattaaga taatatttcc tgttcatcag ccagcctcct gttctccctt
      781 tcttttgtca gtgtaccggt gcatgacaac ataatcacag catgataata ataatcaata
      841 acaataagct gtgtcacgtt tacatcatgg aacggaggtg ctcctgtgac ctttgagcag
      901 aaaaaagccc gggccattgc cctgatggac agcaaaaaga tgtggcggag caattatgcc
      961 cctcctcttc tgcgtattct ctggcggctg ggtatccggc ttccgccatt accctttatg
     1021 ccgttctggc aggtcacggt gctgacgggg ggtttatggg gcatttcctg gggatgtgca
     1081 atgtggttta tctactgggg gccgtcagga atggtggcag gtgaggccat tattataagt
     1141 atcacgggtg gttttttgtt cggactgtgc atggcctctt tccactggtg gcgcaggaaa
     1201 gttaaccggc tgccaccatg ggatgatgtg taaaccgtgc acaggcgtgt tcttacggag
     1261 cggcactttc atttatctgt acgcgaacct ccattcctgc atcgtgacag gcctgcagcc
     1321 actgtgccac ttccagcgga tcgccctccc ggcgtaccac cctgccttct ttattccata
     1381 actgcagaca ggtgctgccg tcgagacgca ccacaaaatc cccacggcag gcctgatacg
     1441 ttatgccgtt cagctgcacc gccagatcag tggcagaagt gccgtctgcg ggcagacctg
     1501 ctgccgggac cagcggcagt acgacgactt ctgccgcacg ctgttctgcc cggaggcggt
     1561 cctgttctgc cagcagcaga ggctgtgcca cggcccggcc ggcgtccgtc agctccactg
     1621 ccagctgcag gttcggagcg cgcagggtac gcagccagcc ggcatcctcc agacgacggc
     1681 aggaggcgcg caggtttggc ccgtagaccg gggcttcccc gcttcgctcc agcacccgtt
     1741 ctatgtcccg cgtcagcacg ggtcctggcc gtttttcatc aagggcagcc agcacaatca
     1801 gcactctccg ctgcagtggc gacggtcggc ggctctgggt cattacctgt catc
//
LOCUS       DAMZII010000298         1807 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMZII010000298 DAMZII010000000
VERSION     DAMZII010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.304"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(355..513)
                     /locus_tag="POA77_004916"
     CDS             complement(355..513)
                     /locus_tag="POA77_004916"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6368217.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHINYLTI"
     gene            complement(745..1070)
                     /locus_tag="POA77_004917"
                     /pseudo
     CDS             complement(745..1070)
                     /locus_tag="POA77_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          536 a          393 c          356 g          522 t
ORIGIN      
        1 agtaatcaac agacttgtat gtaaagtcaa gattaacgtt gctaaagcac gcatcaattt
       61 tctgataaaa ggatggggtt gtttttttcg ataaaaaaaa ttcttgtcta accactggtg
      121 aaataagatt attattaccc cgaattaata ctgagcctaa caatagaata caaaaaaaac
      181 aaaatgcttt aaataagggg agcactataa gttatcctct ttactttatg cttaccaact
      241 catttatgtt actttataac ataacccatc ttgcgttgta aacagactcg ttttagttcc
      301 atgcaccttt tgagatcgtg accaaataat ggcgttagtg atgttcttcc agtgtcatat
      361 ggttaagtag ttgatatggc gttcactgtt atattctgac aaccagtttg ccgccagttc
      421 ccgcttttca tccagcgttc tgaacagaca aaaaccgagt atttttgttc ggtatgtccg
      481 gttaaaactc tcgataaaag cgttctgcgc caacttacct gattgggtaa tctccagttt
      541 tactacatgt ttttctgcgc attacgtccg tgttgaataa aaaaaatcct ggccataatc
      601 catgcgaatc atagctggat agacatggtt ttccgcaatc ctacagagta ccgctaccac
      661 ttgtgaagct ggcagattca gattgttttc aatcgacaac gcctcatagt taaagtcatc
      721 aacgacattg aacgtgtgaa gtggtcaaca aaaactggcc accgagttag agtttttcca
      781 gtatcgattt tccgattcgt ttggggataa cccaccgtta tattcgtgcg gtcttagtgc
      841 gctgtaatat ccaacgatat agaccgttat ggcgtgagcg gcctcgcaga agcttcgtaa
      901 cccaccaccg gcatccattc gttcttcaga ctcctgaaga agcgttccat tgggctgtta
      961 tcccagcagt ttccgcgctg gctcatactc tgcctgatct ggtatcgcca caataactgc
     1021 cggaactgcc tgctcgtatc gctgtggaac atcaccccgc cgggcttacc catccagata
     1081 taggtcacat caccgcacca cacctgtata gcggcgtaat ccccaaaaac gatgctggtt
     1141 gtcagtaaac aagccatgaa tgacagtcgt tcctgaaatc ataagacctg gaggagtgta
     1201 tgctccgtcg cagtaattac cacgatacgc ttgcagaatt ttttccagtt actgaagcgt
     1261 gaacggataa agaaaaagct cttcggaacg cgggaagacg cctccaacga tatttttgaa
     1321 catcgaaatg ttttataaca ataagcgtcg gcatggttcc tgcgaacaga tgtcaacaat
     1381 agaatataaa aacaattatt atcaacaact tggaaggagt aagactaccc gtggcgattc
     1441 aatattagat cattgatgga gatataaatg gctcactttt tatcggttgt tgacgatacg
     1501 tttatcaagt atacatgaaa atttcttcat accagctata tgagacgtta agactaaaaa
     1561 tcttactccc aaaatgacta tgttgttgca gtttggttta ttgactcttg tctatagttc
     1621 accgaatgcc aagatgaggg agcgaggaac tttactgtct acgcctctct tcctggttac
     1681 aaagacaagc gtaaaactca ccatccatta gaactaaagg cactttcctg agcagagttt
     1741 tcatctgcaa gataaaagct attttacagc cgtaaagtcc agtgaaacga aagagaggtt
     1801 agtgaat
//
LOCUS       DAMZII010000299         1790 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMZII010000299 DAMZII010000000
VERSION     DAMZII010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.305"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..888
                     /gene="aac(3)-VIa"
                     /locus_tag="POA77_004918"
     CDS             <1..888
                     /gene="aac(3)-VIa"
                     /locus_tag="POA77_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDI6368218.1"
                     /translation="RKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPHVSLR
                     AVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSWPAFD
                     PDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYGPRSP
                     IARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWVTTSD
                     WDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFGIEWL
                     EARHAAPAAAALKPKQRRD"
     gene            1095..1415
                     /gene="groES"
                     /locus_tag="POA77_004919"
     CDS             1095..1415
                     /gene="groES"
                     /locus_tag="POA77_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6368219.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            1471..>1790
                     /gene="groEL"
                     /locus_tag="POA77_004920"
     CDS             1471..>1790
                     /gene="groEL"
                     /locus_tag="POA77_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6368220.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAV"
BASE COUNT          337 a          604 c          572 g          277 t
ORIGIN      
        1 cgcaaaaacg gcgatttgca cgaacccgcg acggcacccg cgacgccctg gtccaaaagc
       61 gagctggtcc ggcaattgcg cgacctcggc gtgcgctcag gcgatatggt gatgccgcat
      121 gtgtcgttgc gcgccgtcgg gccgctggcg gacggaccgc agacacttgt cgatgcgctg
      181 atcgaggccg tcggccccac cgggaatatt ctcgccttcg tctcgtggcg cgattcgccc
      241 tatgaacaga cgctgggtca tgatgcgccg cccgccgcca tcgcccaaag ctggcctgcg
      301 ttcgaccccg accatgcgcc cgcctacccc ggctttggcg cgatcaacga atttatccga
      361 acctatccgg ggtgtcggcg cagcgcccat cccgacgcat cgatggcggc gatcgggccc
      421 gatgcggcgt ggctggtggc gccgcacgag atgggcgccg cttatggccc ccgctcgccg
      481 atcgcgcgtt ttctcgccca cgcaggaaaa atcctgtcga tcggcgccgg gcccgatgca
      541 gtcaccgcgc tccattatgc cgaagcggtg gcgcggatcg agggcaagcg ccgcgtcact
      601 tattcgatgc ccttactgcg cgaaggcaag cgcgtctggg tcaccacgtc cgactgggat
      661 tcgaacggca tcctcgacga atatgccgcg cccgacggcc ccgacgcggt cgaacggatc
      721 gcccgcgact atctcgcccg caccagggtt gcgcaaggcc cggtcggcgg cgcgcaatcc
      781 cggctgatcg acgcggccga tatcgtttcc ttcggcatcg aatggctcga ggcgcgccac
      841 gccgcgccag cggcggcagc gctgaagccg aaacaacgcc gcgactgagt cgtcgattat
      901 tccggacata tattcctccc tctcgcccag ccgcatagca tcaggcccac aagcccgccg
      961 cctcttcaac aaggatgtcg ggacgttctt cagtaaagaa cagcttggca ccctcgacaa
     1021 ggatgattgc ccgaggcgat caggcggcga atttttgcgc gcgcgcacgg tcgcggcggc
     1081 gcgcgataaa ggcgatgggt agccccacga ggaagatatg cgcggcgatt aacatcaagc
     1141 cgctgtacga ccgcgtcgtc atcaagcgca ccgaggaaga gaaggtctcc gccggtggta
     1201 tcgtgatccc ggattcggcc accgagaagc cgatcaaggg ccaggtcgtc gccgtgggcg
     1261 agggcaaggt gttcgacaac ggcaacgtgc gcgcgccgaa ggtcaaggtc ggcgaccagg
     1321 tgctgttcgg caagtacgcc ggcaccgagg tcaagctcga cggcaccgag tacctggtgg
     1381 tgaaggaaga cgacatcttc gccgtgctgg gctgagcccg ccgcttcgcc gcatcccaac
     1441 ccgcaatcac agaactgagg taattccgca atggctgcca aggatattcg tttcggtgaa
     1501 gacgcccgcg cccgcatggt ccgcggcgtg aacgtgctcg ccaacgccgt caaggcgacc
     1561 ctgggcccga agggccgcaa cgtcgtgctc gagaagagct tcggcgcccc gacgatcacc
     1621 aaggacggcg tgtccgtcgc caaggagatc gaactggccg acaagttcga gaacatgggc
     1681 gcgcagatgg tcaaggaagt cgcttccaag acctccgaca acgccggcga cggcaccacc
     1741 accgccaccg tgctggccca ggccctgatc cgcgagggca tgaaggccgt
//
LOCUS       DAMZII010000300         1713 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMZII010000300 DAMZII010000000
VERSION     DAMZII010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.306"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            67..>1713
                     /locus_tag="POA77_004921"
     CDS             67..>1713
                     /locus_tag="POA77_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368221.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTS"
BASE COUNT          466 a          315 c          467 g          465 t
ORIGIN      
        1 tttcattttt aatgtctgac gactcatctc tttcgtggct gtattgtttg tatatggagc
       61 gtttatatga atagaatcta tcgcgtgata tggaattgca ctctacaggt atttcaggcc
      121 tgctcggaat taactcgcag ggcaggtaaa acatcgacgg ttaatttgcg taaatcctct
      181 ggactgacaa cgaaattcag tagattgacg ctgggtgttt tgctggcact aagcggttca
      241 gcgtctggtg caagtctgga agttgataat gatcagatta ccaatattga tactgatgtt
      301 gcttatgatg cctacctggt tggctggtat ggcactggag tgcttaatat tttggctggc
      361 ggtaatgcct ccttaaccac tattactacc agcgtcattg gcgctaatga ggactcagag
      421 ggcaccgtta atgttttggg tggcacctgg cgattgtatg atagcggaaa taatgcaagg
      481 cctttaaatg tgggtcaatc cggaacgggg acgctgaata ttaaacagaa gggtcacgtc
      541 gatggaggct atttaagatt aggttcttcg acaggaggcg tcgggacggt caatgttgag
      601 ggagaggact ctgttctgac gaccgaatta ttcgaaatag ggagttacgg tacgggctca
      661 ttaaatatta cggataaggg ttacgtcacg agttcaatcg tcgccatttt aggctatcag
      721 gcgggcagta atggccaggt tgtcgttgaa aagggtggcg agtggctaat aaaaaataac
      781 gattcctcaa ttgaatttca aattggtaat caaggaactg gggaggcgac tattcgcgag
      841 ggtggactga ttacggctga aaatacgatt atcggtggca atgccaccgg tgtcggaacc
      901 ctgaatgtgc aggatcaaga ctctgtcatc acggtacgca gactctataa tggatatttc
      961 ggtaatggca cactcaatat ttccaataat ggactaatta ataacaaaga atattcatta
     1021 gtgggcgttc aggacggttc ccacggtgtc gtcaacgtga ccgataaagg gcattggaat
     1081 ttcctcggaa cgggcgaagc tttccgctat atctatatcg gtgatgctgg cgacggtgaa
     1141 cttaatgtct cgcgtgaagg taaagtagat tcgggaatta tcactgcggg gatgaaagaa
     1201 acaggcacag gcaacattac tgttaaggat aagaactccg ttatcactaa tctcggaact
     1261 aatcttggtt atgacggcca cggcgaaatg aatatcagta atgaagggct tgttgtcagc
     1321 aacggaggaa gctcactcgg ttatggagaa accggcgtcg ggaatgtcag catcaccacg
     1381 ggggggatgt gggaggtcaa taagaatgtc tataccacca ttggtgttgc gggcgtcgga
     1441 aacctcaata ttagcgatgg cggtaagttc gtatcgcaaa atattacttt tttgggcgat
     1501 aaagcaagcg gtatcggcac actgaacctg atggatgcga catcatcgtt cgatactgtg
     1561 ggtatcaatg tcggtaattt tggtagcggt atcgtaaatg tcagtaatgg tgccaccctt
     1621 aattcaacgg gctatggatt tatcggagga aatgcctccg gtaaggggat agttaatatt
     1681 tcaacggaca gtctctggaa tttaaagaca tca
//
LOCUS       DAMZII010000301         1651 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMZII010000301 DAMZII010000000
VERSION     DAMZII010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.307"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..341
                     /locus_tag="POA77_004922"
     CDS             <1..341
                     /locus_tag="POA77_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6368222.1"
                     /translation="WHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSII
                     KENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHCR
                     KAFSDAKVFK"
     gene            338..1324
                     /locus_tag="POA77_004923"
     CDS             338..1324
                     /locus_tag="POA77_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368223.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          386 a          334 c          392 g          539 t
ORIGIN      
        1 cctggcacac gcgtttgatg gaagacggcg ttcaggcaat gcgcgattat cttgccggac
       61 tggatatcgc ttcgccagag catcaggttc tgatgaacgt aacagcaaaa agcgaggtcg
      121 ctccgtcaat tatcaaagaa aatctctcat tacacttaac ccatacggtg aaatggactg
      181 aatctctcga tacatttctg aatatgccaa ccccggtagc tttccttgaa ataagtaata
      241 agccctattt ggggaatatg ttaaatgatt ttgctggcgt ggatcagcaa cgagttatgc
      301 attgtcgtaa agcatttagt gatgcaaagg tattcaaatg attcctgatt atttaacttt
      361 tattcgcttt caggataaac gaaatctgat atacatttat gctattggac ttattctgat
      421 aggcttttat tggaaaaatg caggatttac ttttccatca gaggatattg gtgtcgttag
      481 tgggattctg gccctggtgc tgtataattt tatttttgat ctcaaggcgt actgggctta
      541 taaatgcgtc acgaagaata tcgatttttc gtggtttaag aaaaagcaga accacaaaat
      601 agaattattt cttacacaac ctctggtggc aggatttctg tcgttaatca tgttgagtgc
      661 aatgagttgg gggctatacc agcttctacc ctcgttatat gcgctgttcc tgatttcgtt
      721 acttgggccg ttggtcatct ttctgctgtt tcggatgatc cgcaccagtt atgtcaagca
      781 ggtcgctatt tcagtagcga aaaaagtaaa atataaaagt ctgactcgct atgtgctgct
      841 ttcggtgtgc atctcaacgg ttgttaacct gcttactatc agcccgttac gtaacagtga
      901 ttcttttgtg acagaggggc agtggttaac gtttaaatcg ataattgcat tgctcattct
      961 ttgtggcgta gtgttggcga ttaatctgtt ttttctgcgc ttctccaagc ggtacgcttt
     1021 tctgggcagg ctttttttgc aggaaatcga tctgtttttc tccagtgaaa atgcgttgtc
     1081 gacctttttt gccaagccgc tttggcttcg gttattcata ttgctggtta ttgaaatgat
     1141 gtggattacg ctggtgtcgg tattggcaac gcttgtagaa tggcggattt ggtttgaagc
     1201 ctatttttta ctctgctatg taccgtgctt aatttactat tttttccatt gtcgatgcct
     1261 ctggcataac gattttatga tggcatgtga catgtatttc cgttgggggc attttaataa
     1321 gtgaggaagt tacaggaagt gaccagataa cgcatatatg ttctctacta tcttgcgcat
     1381 tttgattgtt gactgagtaa ccagacagtt gatgtgcaca atttcccctc gccctaacag
     1441 acgtgggcgg gggcacccgg tgtgtgcaat atacgaaaaa aaagcccgta ctttcgtacg
     1501 ggctcttctt taaatatggc ggtgaggggg ggattgactc gcttcgctcg ccctgcgggc
     1561 agcccgctca ctgcgttcac ggtctgtcca actggctgtc gccagttgtc gaaccccggt
     1621 cggggcttct catccccccg gtgtgtgcaa t
//
LOCUS       DAMZII010000302         1641 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMZII010000302 DAMZII010000000
VERSION     DAMZII010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.308"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            126..1472
                     /locus_tag="POA77_004924"
     CDS             126..1472
                     /locus_tag="POA77_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368224.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRLRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
BASE COUNT          434 a          353 c          404 g          450 t
ORIGIN      
        1 attcatttga aaggatatca gaagaagata tcggggcgca cagttcatct ctgcctttgt
       61 ttcatggggt gttctttgta tattttactt actgtttaag catgtacata aggagtgctt
      121 tcattatgcc aatgatactt gtaaagaaga atccccgggg caaagtgatt cgggagctta
      181 gcagtgagga agagactgca gtaaagacgg tctgtggact gaaaaagcca gcaaccatgg
      241 cactacataa cctggccaat gatattctcc gggaaatgcg tgagtatgat gcatggttac
      301 agtgtgactg tattccggga gattcgccgg ccatgaattt tgctgctctt aaaaacaata
      361 cgggtactct ttatctgtcc agttttaatc atgagcatgc acctgaatgc cccatgtatc
      421 gtcagttatc cggaaatgaa gaggaaacat cttccggagc aagccggcat cccgttagca
      481 cccgtataaa ttacagaaac tttttaccac ctgatgacag taattctgtt atcagactgc
      541 aggcccgctc tgcttaccat aatggtgaga gctcctccgt tcgcaagaaa cgtctgcgcc
      601 ttgcccgttt actcctgtct cttattgacg atgctgggct gaataaactg gattccctgg
      661 ctaatccccg tatccggact aattatcgtg agtgtcttga tgccatacgg caggttacac
      721 tgcagcagga gtatattcgg ggaagatcct tatctgaaat tatccatttc cggcctggaa
      781 tgtctgaaag gtctcaggag cggttaatgg aaacgctgga gaattcagaa cgacactggc
      841 ctgccagaag aaagcatatg ttctttcaga tttttatggc gcagcacatc tgtcgtgatg
      901 ctgtggaaat tcactgggcg aacggtaata ttcaggtcat cagacctgtg cgggggatca
      961 gtattaatgg tgaagcgcag ggcggaatac gtccccctta ctgggttatt cttgcttttt
     1021 gccggagtgc cgatggcaga atcatctgca gtgaagggta tgctcatgct ctttatcaac
     1081 tgacatgtcc ggtgcctgtg gacagcaaac tggaacgaaa cacgctcact gctcttctga
     1141 atgtggccag ctggcttaaa agaaagccag gtacgccgga attaagtctg gaaaggcccc
     1201 tgtttgatac agaagtttat gttaatggtg aaaagaaata tgtactgccg gatttcattg
     1261 ttacagcaag ggctcctgac ggaaagacgg ccagagtggt catcgaaacg atgggatatg
     1321 aagacagtga ttactgcgcg agaaaatcca ggcagcatac cggcatgaag cagattggtg
     1381 ttctgcatac cgatccaccg aaatggctgg ataacgatca tccccctttt gagaaacata
     1441 tgtacggtgt ttttatgcat ctcaggtact gagatatttt gtggctcagt tctgtaactt
     1501 ttcccgtaac attgtctgtt gttacgggaa agtccggttt ttgtattgca ccagagaata
     1561 cccagactgt gatgctgcca caacgtcagc aggctttctg aacggtttaa caccacttca
     1621 tgttaatgat aatcactatc a
//
LOCUS       DAMZII010000303         1599 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMZII010000303 DAMZII010000000
VERSION     DAMZII010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.309"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..459
                     /locus_tag="POA77_004925"
     CDS             1..459
                     /locus_tag="POA77_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDI6368225.1"
                     /translation="MSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYALMHSISRSQ
                     NVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLSKAR
                     ISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ"
     gene            503..1417
                     /locus_tag="POA77_004926"
     CDS             503..1417
                     /locus_tag="POA77_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368226.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          513 a          288 c          315 g          483 t
ORIGIN      
        1 ttgagtggag agacacaagg gctaatttca gcaggttgct caacgcttga ttctgtcgga
       61 aacaaagcgc agccagaaca taaagatcag atcatggttt atgcgttaat gcattccatc
      121 tcaagaagtc aaaatgtcaa tcatcatgaa ttaattatca ccaaaccggt tgataaatcc
      181 tcaccattgc tggcaaaagc acttagtgac aatgaaaaaa tggcaatatg tgaatttata
      241 ctctaccgaa ccagtaaagc gggtatttac cagccttatt ataaaataaa tctgtcaaaa
      301 gcccgaatat caagtattga ttttgtcaca ccgcatgctg tcctggaaaa agaactggaa
      361 ccacaggaga gaattgcctt tatttatgaa gatatttcct gggagcatac gttagccggt
      421 acgaatgcaa tgagtaaatg gcaggatcgt gtacagtaac ttactgtaat aacataataa
      481 attactattg cggagagata aaatggctgg tttaaggaat aacaacaaca cacagaatgc
      541 acagtgggct gattatgtgg gtgacattct tcggggggct cagccgataa accagctagt
      601 acctcagcac ccttatctta acgacgtccc actcattgat gagttaaggc atcagaacac
      661 tcatcatgtt attctcttga cacttgatgt tgcaaaaaaa atactcagcc caataacatc
      721 atttgattac attcatttta tcactacaca tccttcaggt ataaaagata cactcgcctg
      781 gttagttaac gcgagtaaat taatgactga gtttgatgac aatgggaaaa ttatatttaa
      841 ccttaatgcc ttaaagtata caaaagcaag ttactttgaa atacttggag agaaatatat
      901 aaaaatcaca acatccagcc cctggctttt agaaaagctc ggtaaatata ttttctcttc
      961 acgagcgcct caagttctgg aactcgctat tggctggcga ggagctttat ctgaatcaat
     1021 aaaaggagtt aagttctgta tctggttttc agtagcctgg agaaccatag aatttattat
     1081 gagttcagag cgagatctgg ttaattttct tggcgatttt tcaatggatg tggccaaagc
     1141 agttatagcc ggtggtgttg caaccgctat tggaagcctg gcttcttttg cctgtgttag
     1201 ctttggcttt ccagtaattc ttgtcggagg agcaatttta ctgacaggga tagtgtgtac
     1261 agttgtttta aatgaaatcg atgctcaatg ccatttatca gaaaaattaa aatatgcaat
     1321 tagagatgga ctaaaacggc aacaggaact tgataaatgg aaaagggaaa acatgactcc
     1381 atttatgtat gttcttaaca ctccacccgt gatataagga ttaaccatgc gtttacatgt
     1441 taaattaaaa gagtttcttt cgatgttttt tatggccata ttatttttcc ctgcattcaa
     1501 tgcttctttg ttttttaccg gagttaaacc tctatattca ataataaaat gctccacgga
     1561 aatattttat gattggagaa tgttgattct gtgctttgg
//
LOCUS       DAMZII010000304         1535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMZII010000304 DAMZII010000000
VERSION     DAMZII010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.310"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(207..860)
                     /gene="ribB"
                     /locus_tag="POA77_004927"
     CDS             complement(207..860)
                     /gene="ribB"
                     /locus_tag="POA77_004927"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI6368227.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDLI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(964..1112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1234..1524
                     /gene="ubiK"
                     /locus_tag="POA77_004928"
     CDS             1234..1524
                     /gene="ubiK"
                     /locus_tag="POA77_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDI6368228.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          434 a          394 c          351 g          356 t
ORIGIN      
        1 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
       61 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
      121 aacatcttgc atagcggggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
      181 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
      241 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
      301 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
      361 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
      421 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
      481 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
      541 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
      601 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
      661 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
      721 gccgggaaga tcaaatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
      781 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
      841 gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag
      901 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
      961 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
     1021 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
     1081 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg
     1141 gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg
     1201 gcactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa
     1261 atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg
     1321 gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt
     1381 gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt
     1441 gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca
     1501 gagactctcc ccccaacgct gtaaacattt gtctt
//
LOCUS       DAMZII010000305         1506 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMZII010000305 DAMZII010000000
VERSION     DAMZII010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.311"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            308..748
                     /locus_tag="POA77_004929"
     CDS             308..748
                     /locus_tag="POA77_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368229.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDAL
                     RILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGV
                     IFEGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
BASE COUNT          425 a          361 c          395 g          325 t
ORIGIN      
        1 aaaaaacgtg caggagctgt tgaacgtgaa aacctcgaaa tctggcaaaa atcacggctg
       61 acgaggcttg cagaggatta agccagtata cactccgcta tcgttacgtg actgggtcag
      121 ggcttcgccc cgacacccca aagagtagct aggataaata tctaatggaa tcaatgcgtt
      181 agacgcctaa tataaagata cagaaaggag aataaaaaca ttaacaaaat ctaagggtta
      241 cagttgacat agttatcaat aatgataaca ctgttatcat tattgataac tcaaataggg
      301 ttcaaagatg agtaaggatg tcgagagaat cactcagata aatgaccagg gtgagattat
      361 cggcggcttc gttgccgtta tccgccctaa acaaaaatca gcatttcaga ggcatttcac
      421 catgaatcaa gacgcattac ggatcttggc taaggaatta accggggagc aattcaaggt
      481 cttaatgctg atgcttgcag atctggatta cgaaaacttc attcagatcg cccaagcaga
      541 tattgctgaa actttaggga tgcagaaaac gaatgttagt cgcgccgttc gcgcgctact
      601 ggatgttggg gtgatctttg agggacctaa agttggcaga tcaaaaacat accggctgaa
      661 tgaacaattt ggttggaaag gcaccgtaac caaccacaaa aaggcactca aaaacggctt
      721 tagtgtcatt cagggcggcc ggatttaaag agccagcggc aggcggacaa tcagggctac
      781 gtgttaacgt tttgaccatg attgtctatc ctgcattgct cttttgccgc atgtcagttc
      841 gatgcgatgc aggatagggc aaacgccgca aaatgacgtc attgacgtca ttcgcaacct
      901 ttgcccgata atctccaaaa cgtctgagct gccacggttc cggcagcgcc ttgagcgcaa
      961 ccggaaaccg tctggggaga accccagacc cccatcgctg taacatcgac ggtctacgac
     1021 caccaaccag ctgaatagct gccagtgccg tgcaaatggg ctttgcaggg ggctgacgcg
     1081 gttcctggcg acctgaaagc ccctggttag gggtatcggc gcgcctgacc tcctgaaacc
     1141 ctctgtaagc gtttttcgcg ccttccatgc cctgacgggc atttaaaacc cgtttcgtgc
     1201 gcctgagagc ttctgagagc gtttgaagag ctatctggcg actcatcccc gaaagaacaa
     1261 cagcggaatc accgataaca tgcgagaggt ggcaccgctc gccgcagccc cagcgaccga
     1321 acgttagtga gcgagtgggc gaggaagcgg aagagaaccg gaaaccacat tgagcacgta
     1381 cgcactgatg cggggtgtcg gggtgaagcc ctgaccagat ggcaaacgga atatcgtcgc
     1441 gggtgacggt attacatttg cacatcctgt cccgatttct gcggagttta acggtgaacg
     1501 gacggc
//
LOCUS       DAMZII010000306         1490 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMZII010000306 DAMZII010000000
VERSION     DAMZII010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.312"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            245..649
                     /gene="stpA"
                     /locus_tag="POA77_004930"
     CDS             245..649
                     /gene="stpA"
                     /locus_tag="POA77_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDI6368230.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(696..1220)
                     /gene="ygaP"
                     /locus_tag="POA77_004931"
     CDS             complement(696..1220)
                     /gene="ygaP"
                     /locus_tag="POA77_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDI6368231.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1230..>1490)
                     /locus_tag="POA77_004932"
     CDS             complement(1230..>1490)
                     /locus_tag="POA77_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6368232.1"
                     /translation="AAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSASATSQ
                     HLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          413 a          346 c          347 g          384 t
ORIGIN      
        1 gttttgtcag gcttgcggaa ttagcgagca gagagcgcca ctctgttcac caataatcta
       61 aattactcat caaaatggct aatatataat cttgaaatta ttctgagagt ttcagaaaat
      121 aacagctgaa ataatctcgc gcaggactgt aaatagatta aattttgtgg aaatataata
      181 agtgatcgct tacactacgc gacgaaatac ttttttgttt tggcgttaaa aggttttctt
      241 tattatgtcc gtaatgttac aaagtttaaa taacattcgc accctccgtg cgatggctcg
      301 cgaattctcc attgacgttc ttgaagaaat gctcgaaaaa ttcagggttg tcactaaaga
      361 aagacgtgaa gaagaagaac agcagcagcg tgaactggct gagcgtcagg aaaaaattag
      421 cacctggctg gagctgatga aggctgacgg aattaacccg gaagagttat tgggtaatag
      481 ctctgctgct gcaccacgcg ctggtaaaaa acgccagccg cgtccggcga aatataaatt
      541 caccgatgtt aacggtgaaa ctaaaacctg gaccggtcag ggccgtacac cgaagccaat
      601 tgctcaggcg ctggcagaag gtaaatctct cgacgatttc ctgatctaat tactgaggcc
      661 ggatgtcgct gctagggcgc gtccggcgtc gcttctcaag ctcgttggtt ccacggcatc
      721 ttatctaaca accttgccat tccacaaaaa ccgctgattc ctgcaaacag taacccggca
      781 ccaacaaagc cacttaataa gaagaaaccg ctatttacgg tataacccag tacaacgccg
      841 attaatatta aaccacccgc agcgatctgc acctggcgca ttaacggcaa gggttgagat
      901 ttatttaccg ctactggcaa tccagctctt ttccagccat caatcccatc ttcgagtaaa
      961 aatatttctg cgggggcggc aatcgctgct aatttatcgg cgttattact ggtgcgttta
     1021 cctgcctggc agtggaatat aatttgctcg tgacgtaatt tagccggaag acctgactgt
     1081 tccagcacgg ataatggagc cagatctgct tcaggaatat gttcacgaag atattcatca
     1141 gcatcacgaa tatcgattaa ctttgcgccg cgtgcgatta attcttgtgc atcatgcggc
     1201 gaaatggttg tcaaagccat agtgactcct tacggacaat agacattttt cagggtggcg
     1261 ataatggcat ttaccgcctc atttttaatg gaatatagaa tgcgttgggc atcccgttgg
     1321 ctgtcgataa gcccttcgtc ccgcatacga gcgagatgct gtgatgtcgc agaggcactc
     1381 agtccggtaa tgcgcgtcag ctctcccgcg ctggtgccgg gggaaccgct aagcatgcac
     1441 agaatcagca atcgtttagg atggctcatt gctttcaata aggccgctgc
//
LOCUS       DAMZII010000307         1481 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMZII010000307 DAMZII010000000
VERSION     DAMZII010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.313"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..146
                     /locus_tag="POA77_004933"
     CDS             <1..146
                     /locus_tag="POA77_004933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368233.1"
                     /translation="TLRDQPPHGLAIKGVKCHGTRPACLHGLAIMGVKSHGTRPAGTV
                     VIG"
     gene            complement(857..1285)
                     /locus_tag="POA77_004934"
     CDS             complement(857..1285)
                     /locus_tag="POA77_004934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368234.1"
                     /translation="MALSPLNRQTMEGSWLRTVAFNPLNRQTMEESWACPMEFNPLNR
                     QTMEESWSCPVAFNPLNRQTMEESWLRTVAFNPLNRQTMEESWSCPVVFNPLNRQTMG
                     GSWSGTVAFNPLIGRPWRDYCRVPGHLTTYSSVHDGMLVA"
BASE COUNT          390 a          354 c          339 g          398 t
ORIGIN      
        1 ccacgctacg cgaccagcct ccccatggcc tggcgattaa gggggttaaa tgccacggta
       61 cccgaccagc ttgcctccat ggtctggcga ttatgggggt taaatcccac ggtacccgac
      121 cagcaggcac cgtggtcatt ggctaaaggg gaaattgcct cggcttgcag acaggacaac
      181 gtgacgccct agcggttaag tgggtttaat acgacggtac acaataagta tcccaccaga
      241 atctgtctat tgagtggatt agattaaaag atattcggcc tggatctcga cttagaatga
      301 cgaaatattt ggcaaaaaaa tagggtattc ggcaaatcct gttgttagga catcccgaat
      361 acccaaaaaa tatttggcaa tcaatatgaa ttgcccatac ccccaattga caataagaga
      421 gttttttggc aattcgtata attaggccaa ttcatccgtg tcgtctattg cactaacgaa
      481 gtattccgtg cggtagtcat ctccacccct caactagact gaaggtgatc catcttttgc
      541 aagcgtacgg tcacctgaca tgctgtccgc tcgtacagga attgtcatgc attctttaat
      601 ggtatctgct tcagccacgt gtccagtaca ctggttttca aatccagcaa ctactacata
      661 cgcatccggg tcgagccttt taagctggtc tataagctct tttactttta tgggttacct
      721 ctacgttttt gtatatttta tcacgctgaa cggaagggcg aataactgta gtgaaacaaa
      781 aaaagttttg gccggaaaac cttttagact ggctttcctt tagcaaagaa ctggtgaata
      841 ggggagcaat tccgcgctac gcaaccagca tcccatcgtg tacagacgag taagtggtta
      901 aatggcctgg tacccggcaa taatccctcc atggtctgcc gattaagggg ttaaatgcca
      961 cagtacccga ccagcttccc cccatggtct ggcgattaag ggggttaaat accacgggac
     1021 acgaccagct ttcctccatg gtctggcgat taagggggtt aaatgccacg gtacgcaacc
     1081 agctttcctc catggtctgg cgattaaggg ggttaaatgc cacgggacac gaccagcttt
     1141 cctccatggt ctggcgatta agggggttaa attccatggg acacgcccag ctttcctcca
     1201 tggtctggcg attaaggggg ttaaatgcca cggtacgcaa ccagcttccc tccatggtct
     1261 ggcgattaag ggggctcaat gccacggtac cctaccaact ttctaccatg gtcagtcgat
     1321 tcaagtggtt aaatttcgtt attgaataag cttaaaatgg tttcttattc caaccagaaa
     1381 gttagatggt taagttatta tggagataag acctgatctt acttgttttt tggttgtttt
     1441 aacaaaccat tcaccttaat aatatctacg catcgcataa c
//
LOCUS       DAMZII010000308         1470 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMZII010000308 DAMZII010000000
VERSION     DAMZII010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.314"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(101..1309)
                     /locus_tag="POA77_004935"
     CDS             complement(101..1309)
                     /locus_tag="POA77_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6368235.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT          384 a          385 c          291 g          410 t
ORIGIN      
        1 catttatgtc attgcaaata tcagacaaac aagcattata actcaataag tatatttttc
       61 gtgattacag acgacatctg caaatctgtg agatgtgatt tcatcgctgg ttttccataa
      121 tctgagacat cacataatcc gcccatgcct gcatcatcgg tatacgctga tccagcagat
      181 cagtccggtg atatgccgcc tcaaccttat ttttcagcgt atgggcaagc gccctttccg
      241 ccaaatcccg cgaatatccc tgttcgctac accagtccct gaatgttgaa cgaaaaccat
      301 gtgctgtagc cactcgtccg ggtatatcac tgatggcctt ctttttacgc agaaaactcg
      361 tcaacaccat atctgaaagg atctgctgct ttctgggaga aggaaacacc agttcgtcat
      421 gcagaccacg tatattttcc agaacgtgaa tggcctgtcg ggataaagga acacgatgct
      481 gtatcctggc tttcattctt tctgcgggta tcgtccatat ccgcttatga aaatcaattt
      541 cagcccagcg cattcccctt gcttcgcccg agcgggttgc tgtcagtatc accattaata
      601 acagtgcccg ggtaacatta taaggttcat ctgtatacac actggtcgcc acaaaaagcg
      661 gtaactgcct ccagggcatt gccggctggt gttcatcacg tcctcttgat tgctgtggaa
      721 gcaaatgatc aaccacatca acaggattcg ccacacaaaa accatgcgcc cagccccact
      781 gcataacaac atgaatgcgc tgtttaaccc ggcttgccgt ttctgacaag gttaaccaga
      841 ctggacgcag tgtttctgcc acgtccgcag ccgtaatcga atccagcgtt tttgctccca
      901 gttgaggaaa cgcgtaattc tcaagcgtcg ataaccactg ccttacatgc tttggatttt
      961 cccatccagg agacagttct gcatgtacac gcctggctgc atcggcaaat gttgggataa
     1021 caactttttc agcttcagcc tttttaatct ccagaggatc atcacctgca gcaagttgct
     1081 ctcgcattat ccgggcagta cgtgcagctt cagcaatact gacctcagga taagttccca
     1141 atccagcatt acgccttttt tgtgtcaccg gacttacata acgaaaaacc catttacccc
     1201 tcccctttac tgaagaagga tgaagagtca gtcctgtaat tcccccatgg ggcaatggtt
     1261 tgtcatcagg tttgatatgc cttgctttag tatccgtcaa tactgccata cgctaattcc
     1321 tgttactctg gtatgccatt cagtatgcca ttagtctctc acttcactaa gaatacatta
     1381 gataacatcg gacagaaaaa attgtaatgc attgtttata ataaggttat ataacactcc
     1441 cggattaagt cagatttatt tcaggcggtc
//
LOCUS       DAMZII010000309         1455 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMZII010000309 DAMZII010000000
VERSION     DAMZII010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.315"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            144..1355
                     /gene="bluF"
                     /locus_tag="POA77_004936"
     CDS             144..1355
                     /gene="bluF"
                     /locus_tag="POA77_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDI6368236.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          422 a          303 c          301 g          429 t
ORIGIN      
        1 agatttttcg ccaactagtg ttcaactttt tccatttttt atcaatgtaa agaaacggtt
       61 atgttttggt attaaaagaa agacacaaga agatatgtct gttaccgtct tactctcgcc
      121 tcacccatta ccctggattg ttaatgctta ccacccttat ttatcgtagc catatacgtg
      181 acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc aggaacatgc
      241 agtctgacgt aacagggatc ttactgttta atggttctca ttttttccag cttctggaag
      301 gtccggaaga acaggttaaa atgatatatc gggctatatg ccaggatcca cggcactata
      361 atattgttga gctgctgtgc gattacgcgc ctgctcgccg ttttggcaaa gcgggaatgg
      421 aattatttga tttgcgcctg cacgagcgag atgacgtttt acaggccgta ttcgacaaag
      481 gcacatcaaa atttcagcta acttatgatg acagagcgct acaatttttt cgtacttttg
      541 tccttgcaac cgaacaatca acctatttcg agatccctgc cgaagactcc tggcttttta
      601 tcgctgacgg atctgataaa gaacttgatt cctgtgccct ttcaccaact ataaacgacc
      661 actttgcctt tcatcctatt gtcgatccct tatcgcggcg gataatcgct tttgaagcca
      721 ttgtgcaaaa aaatgaagat agcccatcag ccatagcggt tgggcagcgt aaagacgggg
      781 aaatctacac agcggatctc aaaagtaagg cgcttgcatt cacgatggca cacgcacttg
      841 agctcggtga taaaatgatt tcaatcaatc tattacctat gaccctggtt aacgaacctg
      901 acgcagtctc ttttttactt aatgaaataa aggccaatgc tctggtgcct gaacaaatca
      961 tcgttgaatt tactgaaagt gaagtcatat ctcggtttga tgagtttgcc gaagcgatta
     1021 aatcgctaaa ggctgctggt atcagtgtag caattgatca ttttggcgca ggttttgctg
     1081 gtttgttact cctgtcacgc ttccagcctg acagaattaa aatcagtcag gaattgatta
     1141 ccaatgttca taaaagtggg ccacggcagg caattattca ggcgatcata aaatgctgta
     1201 catcacttga aattcaagtc agtgctatgg gcgtggcaac accagaagag tggatgtggc
     1261 ttgaatccgc aggaattgag atgtttcagg gagatctgtt tgctaaagct aaattgaatg
     1321 gtatcccttc catagcgtgg ccagagaaaa aataattttc agcacattct ttcacatgat
     1381 ttcagtaaat catcgtgaaa atgaatacgt tacgtttaaa tttaatccac attaaaaata
     1441 atgtttcatc caatt
//
LOCUS       DAMZII010000310         1437 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMZII010000310 DAMZII010000000
VERSION     DAMZII010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.316"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            23..1288
                     /locus_tag="POA77_004937"
     CDS             23..1288
                     /locus_tag="POA77_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDI6368237.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT          339 a          373 c          403 g          322 t
ORIGIN      
        1 tgaaagttaa tgagaggtgg ttatggcttc tacttttacc agcgacacat tgcctgccga
       61 tcataaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg gcgacgtcca
      121 gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa tcaacgcact
      181 caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca aagcaggtca
      241 tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga taaaaggcca
      301 tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc tggaccgcaa
      361 ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc tcagcgcttc
      421 acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc gccagagtga
      481 agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa gtgatggaaa
      541 gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc gccgtccgcc
      601 cggaacgacc tccaaaggtc ttggagcgca taccgactcc ggggcgctgg aacgctggct
      661 gcttccagcg tatcagcgcg ttttcgccaa cgtctttaat ggcaatctgg cgcaatatga
      721 tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca acaccaccaa
      781 atgttccgtg tttcggacat tccagggctg gacagcgctc tctgatatgc tgcctggtca
      841 ggggctgctg cacgttgtgc ccattcctga agccatggcg tacgtactgt tacgtccgct
      901 gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag tgttgccggt
      961 atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa aactcgaggc
     1021 cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg ttgaaaatca
     1081 acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga aaaatcttgc
     1141 ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg acttcccgcg
     1201 cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca acattcacgg
     1261 taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat gtgggattaa
     1321 gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat gcctgatgcg
     1381 gcgttaacgc cttatctggc ctacaaagtc ctgcagattc aatatattac aggtact
//
LOCUS       DAMZII010000311         1393 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMZII010000311 DAMZII010000000
VERSION     DAMZII010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.317"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            28..1386
                     /locus_tag="POA77_004938"
     CDS             28..1386
                     /locus_tag="POA77_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6368238.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          394 a          223 c          314 g          462 t
ORIGIN      
        1 ctacaaccta cagattggtg tagctttatg gaaaaagact atttgagaat tagtagtact
       61 gtattagtga gcttattgtt tgggcttgct ttggttttgg tgaatagttg gtttaatcag
      121 ccaggcgttg aggaagttgt tccacgatca acctatctga tggtaatgat tgctttattt
      181 tttatcgata ctgttgcatt tatttttatg cagttgtatt tcatttatga ccgtaggcaa
      241 ttttcaaact gtatacttag tttggctttt ctgagttgtt tgatttactt tgttaaaacc
      301 gtcattatca ttcagcaaat tattgagggg cgtttgacaa gcagtgttgt ccaaaatgac
      361 attgcaatct attatttgtt tcgtcagatg agtttgtgca tattaatatt tctggcattg
      421 gtgaataaag ttagtgaaaa cacaaaacag cgcaatttat tttccaaaaa aatgactttg
      481 tgcataagtt tgttttttgt ttttgggggg ccgatagttg ctcacatact ttctagtcat
      541 tatgagagct acaatttaca cattgccgag ttaaccaatg aaaatggtca ggtggtttgg
      601 aaagcctcat atgttaccat aatgattttc atgtggttaa ccttgctttc ggttaatcta
      661 tactttaatg gtttacgcta tgatatctgg aatggagtga cggtgattgc tttttgtgcc
      721 gtactgtaca atatctctct gttatttatg agtaggtata gcgtgtccac ttggtatatt
      781 agtcgcacta ttgaagtagt cagtaagtta actgttatgg taatatttat gtgccatata
      841 ttcagtgcgc tacgagtaac aaagaacatt gcacatcgcg atcccttaac caatatattt
      901 aacagaaatt atttttttaa tgaactgaca gttcaatcag catcagccaa aaaaacgcct
      961 tattgcgtca tgattatgga tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat
     1021 ccggttggcg atcaggtgat aaaaacagtg gtgaatatca ttgggaaaag catacgacca
     1081 gatgaccttt tagcgcgcgt cggcggcgaa gagtttggcg tcttgctgac ggacatcgat
     1141 actgaacgtg cgaaagcttt agcggaaagg attcgggaaa atgttgagcg tttaactggc
     1201 gataatcctg aatacgctat accacaaaaa gtgacgatta gtattggcgc tgttgtgact
     1261 caggaaaatg cgttaaaccc aaatgagatc tatcgactgg ctgataacgc actttacgag
     1321 gcgaaagaga ccgggcgtaa taaggtggtt gtgagggatg tggtgaattt ttgtgagtca
     1381 ccataaagcg gca
//
LOCUS       DAMZII010000312         1372 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMZII010000312 DAMZII010000000
VERSION     DAMZII010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.318"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            133..948
                     /gene="cma"
                     /locus_tag="POA77_004939"
     CDS             133..948
                     /gene="cma"
                     /locus_tag="POA77_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HDI6368239.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDHRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(998..1351)
                     /gene="cmi"
                     /locus_tag="POA77_004940"
     CDS             complement(998..1351)
                     /gene="cmi"
                     /locus_tag="POA77_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HDI6368240.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT          386 a          310 c          257 g          419 t
ORIGIN      
        1 ctgccctacc atttttaacg gcatatcagg caaacaatgt tatggggagc ctctctcatg
       61 gggtttgttc gccggcggag gcgttagcca gcggcgaact ctgttatctt gttaacttat
      121 aaggagttat gtatggaaac cttaactgtt catgcaccat caccatcaac taacttacca
      181 agttatggca atggtgcatt ttctctttca gcaccacatg tgcctggtgc tggccctctt
      241 ttagtccagg ttgtttatag ttttttccag agtccaaaca tgtgtcttca ggctttaact
      301 caacttgagg attacatcaa aaaacatggg gccagcaacc ctctcacatt gcagatcata
      361 tcgacaaata ttggttactt ctgtaacgcc gaccgaaatc tggttcttca ccctggaata
      421 agcgtttatg acgcttacca cttcgcaaaa ccagcgccaa gtcaatatga ccatcgctca
      481 atgaatatga aacaaatgag cggtaatgtc actacaccaa ttgtggcgct tgctcactat
      541 ttatggggta atggcgctga aaggagcgtt aatatcgcca acattggtct taaaatttcc
      601 cctatgaaaa ttaatcagat aaaagacatt ataaaatctg gtgtagtagg tacattccct
      661 gtttctacaa agttcacaca tgccactggt gattataatg ttattaccgg tgcatatctt
      721 ggtaatatca cactgaaaac agaaggtact ttaactatct ctgccaatgg ctcctggact
      781 tacaatggcg ttgttcgttc atatgatgat aaatacgatt ttaacgccag cactcaccgt
      841 ggcattatcg gagagtcgct cacaaggctc ggggcgatgt tttctggtaa agagtaccag
      901 atactgcttc ctggtgaaat tcacattaaa gaaagtggta agcgataata taagggccgg
      961 ggttccggcc tttttatgca gcctttataa cagtgtatta gtcattcgca tattcaacat
     1021 atccctcctc acaatggtac atgccataat acattctctt accctccggg ccttcagctt
     1081 taaatgggac aagaagtgtt ttttcatctt caacatctgt taaacttctc tcaatctttt
     1141 cccatgtaat tttcgggatt tctgtaccaa gaagttgtac atcgttgtcc cagcgtttca
     1201 ttctttgcag gtaatcattt tttacaaacg ttctggcctg ttcatcgctg acctgatagc
     1261 atgccggacc tttatcttca gaccagaaaa aaacagcagc aagagcaata atcgttaaaa
     1321 taaaaataaa tttcatgcta attactttca taaaatctcc acaacttctg tc
//
LOCUS       DAMZII010000313         1371 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMZII010000313 DAMZII010000000
VERSION     DAMZII010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.319"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1270
                     /locus_tag="POA77_004941"
     CDS             <1..1270
                     /locus_tag="POA77_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6368241.1"
                     /translation="NSPYTVYECLINNVNFLASNVGGIPELIQQEHHAEVLFIPTPVD
                     LYWKIHYRLKNINIKPGLAESQDNIKEAWFVAVERKNNRAFKKIDEANSPLVSVCITH
                     FERHHLLQQALASIKSQTYQNIEVILVDDGSTTEDSHRYLNLIENDFNSRGWKIVRSS
                     NNYLGAARNLAARHASGEYLMFMDDDNVAKPFEVETFVTAALNSGADVLTTPSDLIFG
                     EEFPSPFRKMTHCWLPLGPDLNIASFSNCFGDANALIRKEVFEKVGGFTEDYGLGHED
                     WEFFAKISLQGYKLQIVPEPLFWYRVANSGMLLSGNKSKNNYRSFRPFMDENVKYNYA
                     MGLIPSYLEKIQELESEVNRLRSINGGHSVSNELQLLNNKVDGLISQQRDGWAHDRFN
                     ALYEAIHVQGAKRGTSLVRRVARKVKSMLK"
BASE COUNT          414 a          256 c          294 g          407 t
ORIGIN      
        1 aaactcaccc tatactgttt atgaatgctt gattaataac gttaatttcc tcgcttcaaa
       61 cgttggtgga attccagagc ttattcagca ggagcatcat gcggaagttc tatttattcc
      121 tacacctgtc gatttatact ggaaaatcca ctatcgctta aaaaatataa atataaaacc
      181 agggcttgct gaatcacaag acaatattaa agaagcttgg tttgtcgcag ttgaacgaaa
      241 aaacaaccgc gcattcaaga aaatcgatga agctaacagc ccgttagtta gcgtgtgtat
      301 aactcacttc gaacgtcacc atttgcttca gcaagcactc gcatcaataa aatctcagac
      361 gtaccaaaat attgaggtca tcttggttga tgatggaagt acgacagaag attctcatcg
      421 ttatttaaat ctcatcgaga atgattttaa ctctcgaggc tggaaaattg tccgtagttc
      481 taataactat ctgggtgctg caaggaattt ggctgcgcga cacgcctctg gcgaatatct
      541 gatgtttatg gacgatgata atgttgctaa gccttttgag gtagaaacgt ttgttactgc
      601 agcattaaac tctggggccg atgtgttaac cacaccaagc gatcttattt ttggtgagga
      661 gttcccttct ccgttccgta aaatgacgca ctgctggctt ccgttagggc ctgatttaaa
      721 tatcgccagc tttagtaact gctttggcga tgctaatgcg ctgatcagaa aagaggtttt
      781 tgaaaaagta ggcggattta ctgaagatta cggtttaggt catgaagact gggagttttt
      841 tgccaaaata tcattacagg gatataaatt gcaaatcgtc ccggaacctc tattttggta
      901 tagagttgca aactccggca tgttgttaag tggaaataag agtaaaaata actaccgcag
      961 tttccgtcct tttatggatg agaatgttaa atataactat gcaatggggt tgataccttc
     1021 ctacctcgag aagattcaag aacttgagag tgaagtgaat cgcttgcgga gcatcaatgg
     1081 tggtcattct gtcagtaacg agttacaact tttaaataat aaggttgatg gtcttatttc
     1141 tcagcaaaga gatggctggg cccatgaccg ttttaatgct ctgtatgaag caattcatgt
     1201 ccaaggcgca aaacgaggca ccagcctggt tcgccgggtt gcccggaaag tgaaatcaat
     1261 gttaaaataa agcaatattc ggggccagaa aaggctccga aaaaataata ttaactttca
     1321 gctaatttta aatttgtctg taaataaccc tgttttagtt gcatgtattt t
//
LOCUS       DAMZII010000314         1365 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMZII010000314 DAMZII010000000
VERSION     DAMZII010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.320"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..716
                     /locus_tag="POA77_004942"
     CDS             <1..716
                     /locus_tag="POA77_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004860215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDI6368242.1"
                     /translation="LLHLFESLTTEQKIDIARRLLQNKEIKSTTVISSEEDKAISFSQ
                     PYVFAFTSNFRVFSDLDISKNEFRVLTYILEYMEFGNLINLSQSSLCKALNIASGNMS
                     NIFKKLRQKGILVEHHGHLYINSNIFAKGMSHQLDQKRKEQLSNARNLCAEIEQYPEN
                     YTKHSQEERRKLTLETEVKEIYTDAFVFSKKKKNNAVPNPNNSIHVDKPRKVYKRTKE
                     NEEARRNSVDIESHMQKVS"
     gene            744..>1365
                     /locus_tag="POA77_004943"
     CDS             744..>1365
                     /locus_tag="POA77_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368243.1"
                     /translation="MKKLPGSLEIKLHEKLSKSDILNILAEQMTMLEETFGIQEFKIF
                     SYLECYIGDKKQALYYRSRNSAVATFKLKGLESPVNTAKLISKENGQRIVSFDKELDI
                     DRISATVRNIQNNNPYQGWSEGISVVPASIISKIIEVDIIRAQEEQGRLNRIEEQRKK
                     EEQIRKAKEREEYERPLKAFISSKIKESGLSEKDFKKQVCSSCDYLK"
BASE COUNT          520 a          234 c          256 g          355 t
ORIGIN      
        1 acctattaca cttatttgaa tcattaacta cagaacaaaa gattgatatc gcaagacgct
       61 tgctacaaaa caaagaaata aaatcaacga cagttatttc ttctgaggaa gataaagcta
      121 tttcgttttc acaaccttat gtgtttgctt ttacttccaa ctttcgagtt ttctctgatc
      181 ttgacatatc caagaatgaa ttccgtgtat taacctatat cttggagtat atggagtttg
      241 gaaatctgat caatctttcg caatcatcgc tgtgcaaagc attgaacatc gcttccggta
      301 atatgtctaa catttttaaa aaactcagac agaaaggaat actcgtcgag catcatggtc
      361 atctctatat caactctaac attttcgcaa aagggatgag tcatcaactg gatcagaaac
      421 gtaaagaaca gttgagcaat gctcgcaatc tttgcgcaga gatagagcag tatccagaaa
      481 actatacgaa acattcacag gaagaaagaa gaaaacttac attagaaaca gaggttaaag
      541 aaatctatac tgacgcattt gttttcagta agaagaaaaa gaataacgct gttcctaatc
      601 ccaataacag catacatgtt gataaaccac gaaaagtata taaaagaaca aaggaaaacg
      661 aggaagcaag gaggaactct gttgacatag agtctcatat gcagaaagta tcctgataaa
      721 ataacaacaa caaggaaata acaatgaaaa aactacccgg ttcgcttgag attaagctcc
      781 acgaaaaact atccaagagt gatatactaa atatattggc tgagcagatg accatgcttg
      841 aggaaacatt tggcattcag gagtttaaga ttttctctta tcttgaatgc tatattggag
      901 ataagaaaca agcattatat tatagaagtc gcaacagtgc cgtagccaca ttcaaattaa
      961 agggtctgga atccccggta aacacggcta agctcatatc aaaagaaaac ggacaacgta
     1021 ttgtttcctt tgataaagaa ctggatatag acagaatatc tgctacagtc cgaaatatac
     1081 aaaacaataa tccttatcag gggtggtctg aaggtatcag tgttgttcct gcctcaatca
     1141 ttagtaaaat aattgaagtg gatataatac gtgcgcagga agaacaaggc agacttaatc
     1201 gtattgaaga acagcgcaag aaagaagaac aaatacgcaa agcgaaagaa agagaggagt
     1261 atgaacgacc tttaaaggct tttatttctt caaaaataaa agaaagtggt ttatctgaga
     1321 aggatttcaa aaagcaggtt tgttcaagct gtgactatct gaaag
//
LOCUS       DAMZII010000315         1357 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMZII010000315 DAMZII010000000
VERSION     DAMZII010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.321"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            63..872
                     /locus_tag="POA77_004944"
     CDS             63..872
                     /locus_tag="POA77_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6368244.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSVINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            865..1347
                     /locus_tag="POA77_004945"
     CDS             865..1347
                     /locus_tag="POA77_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368245.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
BASE COUNT          472 a          239 c          252 g          394 t
ORIGIN      
        1 ataccaatat acttagcaaa ctatgtgatc tccatttcga ttgatttagt gtttattgac
       61 gtatgtactg gattattaac gataatatcg agttctggcc tgagcaccga aaattaatat
      121 cggtacataa cgccgatctt aacgtcgttc tgacaacgcc agccagtcga tgtttatcac
      181 ttctacttga agcttttcct gatgtggttg cacaacaaga ttttttcacc agagtctggg
      241 aagaagaagg tatgcgtgtg cctactaaca cgttatatca gaacatatcc attatcagac
      301 gcggatttcg cgctgttggt gatactaccc actcgctaat tgcaaccgtg ccgagaagag
      361 gattcaagat ccataatgac attaacatac aaaatcatgt aataaactcg tcaacagacg
      421 cacatacaca caatgcccca cctgccataa aagttaatgc gggatacaaa gagagcattg
      481 gtggcgcaaa gaatttcaat aacaaaatcc tcaaacatat aaaaagtcat ctaattatgt
      541 tgagcgcatt tgtcataggc gcatattctg catattggct atggaataat aatcaaccga
      601 agccattttt caaggattac aagactgttg cggaaattaa tggctgccat tttaatgtaa
      661 cagaagatac aattgacggg ttgaaagagt tcgataaata taaaacacgg atactggatt
      721 cggtgattaa ctgtaaaaaa catccgtggt tatactttcc tcttgcaaaa tcctcaccgg
      781 ggatgattgt tatggcatgc aataaaaatt ataaccaaca tgaagtggca gactgtttaa
      841 ccctgtctta ccgtgaggtt aatcgtgatt gattataaaa aaaacttatt atttatttta
      901 gtttttatca gtgggtttat tctcttcact gtctattctt acactgcaga aaaaatgata
      961 tataacgaaa catgcactgc aaattgggta atttttaatg accagggacg agcaaatctt
     1021 accatcgact ttatgtataa caaaaaaaat aaaacaggaa ctgttgcgtt gagtggaacc
     1081 tggcaacaag gtaacagaga aagtaaatca atacggcgaa atattgaata cacatggatt
     1141 gaaaactatg acacagccca tttaacatca aagaaagtta ataagtttga gattatggat
     1201 caagttgatg atgatagact tgcacaactt attcctgatt tttatgtctt tccagaaaaa
     1261 agtgtaagct ataatatact aaagcaaggt aagcatgctt ttattttgag cattggtaac
     1321 agagcaataa tgcattgtgc aaggtaaact aaaatag
//
LOCUS       DAMZII010000316         1348 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMZII010000316 DAMZII010000000
VERSION     DAMZII010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.322"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..350)
                     /locus_tag="POA77_004946"
                     /pseudo
     CDS             complement(1..350)
                     /locus_tag="POA77_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
     gene            complement(571..1302)
                     /gene="bluR"
                     /locus_tag="POA77_004947"
     CDS             complement(571..1302)
                     /gene="bluR"
                     /locus_tag="POA77_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDI6368246.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          345 a          312 c          305 g          386 t
ORIGIN      
        1 atggcgtagt aactgcgttt gttatgatta acatcagtaa cccaccggaa aacgcccgcg
       61 cctgctagtg ttgaacagta ttcccgaaat gtagattttc cgcaaatatg aagcaatgcg
      121 gcctatttta ttttagcagg gttcttggtc gtactaactt ttaacaggtt cctggttcct
      181 cttaataaca aaaccgtgtc atcgtgagta ataattctga tgttatccgt agccagataa
      241 taaatgtaat gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc
      301 tcttttctca cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata
      361 acctcttgtt agttgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg
      421 tctgtccttt ggctgtgata tgtacagagt gtgatagagg gaatatctga attctcccgg
      481 tgagcatttt gcaacggacc agctccggta caaacgctgt tgtgggttca gattataaca
      541 ttctgtctaa ggggcgggat aaaggtgaaa ttagggggca tgaaagatga ctttataacc
      601 ttgctcaccc caatgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca
      661 gacaatcaat gtttgcgtcg ggaacagttc cgggcgcggc gattcaatgg gatcagcaag
      721 aacagaaatg tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct
      781 ctcttcaaca ttccacgcca tcagaatggc ttctttaccg ggcttacggc gcatttcgaa
      841 aagcgaggtt gctgcgtatt caattaatgc gccgtcaaac atactgctca taatgcggga
      901 ggtgttgtga tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg
      961 atctactggg tactctcgac ccagtgaaat aattctcgcg cgtagtttgg caggattagc
     1021 catgcgaaga attgacatca tctcttcttg caggcggctc cagtcatctt ccgtatcctg
     1081 gctggtggtt tccagtaatg ctttaacttt gcctacaggg acgccattac ttatccaacg
     1141 cttgatctct tcgatgcgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact
     1201 gcgctgtggt tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat
     1261 cccgcaacgt tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca
     1321 caaatctccg tcgcctgtac acgaccca
//
LOCUS       DAMZII010000317         1317 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMZII010000317 DAMZII010000000
VERSION     DAMZII010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.323"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..152)
                     /locus_tag="POA77_004948"
     CDS             complement(<1..152)
                     /locus_tag="POA77_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368247.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCY"
     gene            233..1317
                     /locus_tag="POA77_004949"
                     /pseudo
     CDS             233..1317
                     /locus_tag="POA77_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          484 a          180 c          310 g          343 t
ORIGIN      
        1 tgataacaat ttgaaaaatg aagtttttga ctggattggt aaatggtcat aatgaacaac
       61 accagaaaga gaaaaatata aaatacatac agaaagacat gagagcaagg gacggggcag
      121 ggcaagatca tgaaaattgt gatgtaaatc acgattttca tcttggcttt aacgcctaca
      181 ggtgatcaaa atttaacctt tggaataact aaaaagataa aaaaggaccc aggtgagtat
      241 tcaaagtttg cttgattata tttcagtgat ccctgatata cgacaacaag gaaaggttaa
      301 acataaatta tctgctattt tgtttctcac cgtatgtgca gtaattgcag gtgccgatga
      361 gtggcaggaa attgaagatt ttggacatga aagacttgaa tggctaaaga aatatggtga
      421 ttttgataat ggcattccgg tcgatgacac cattgcacgc gttgtgagta acattgacag
      481 tttggccttt gaaaagatgt ttattgaatg gatgcaggag tgccatgaaa tcactgatgg
      541 tgaaattata gcaatagatg gaaagaccat aagaggctcc tttgataagg gaaaaagaaa
      601 aggagcaatc catatggtga gtgcattctc gaacgaaaat ggtgttgtac tggggcaggt
      661 gaaaacggaa gccaaaagta atgagattac agccattcca gagttgctta acctactgta
      721 tttaaagaaa aatttgataa ccattgatgc tatgggctgt cagaaagata tcgcttcgaa
      781 gatcaaagat aaaaaagcag attatcttct ggcagtaaaa ggcaatcagg ggaaattaca
      841 tcatgcattc gaggaaaaat ttcctgtaaa tgtgttttct aattataaag gcgattcgtt
      901 tagtacgcag gagataagtc atggaagaaa agaaacacgt ttgcatattg tcagtaacgt
      961 aacgcctgaa cttttgtgat tttgaattcg aatggaaggg attaaaaaag ctttgtgtag
     1021 cattgtcatt caggcagaag aaagaagata aatcagcaga aggtgtaagc atccgatatt
     1081 atatttcatc aaaggatatg gatgctaaag aatttgcaca tgctatcaga gcgcactggc
     1141 tgatcgagca cagtcttcat tgggtgttag atgtaaaaat gaatgaagat gccagccgga
     1201 taagaagagg aaacgcagcc taaataatat ctggaataaa gaagatggca ctgaatttat
     1261 taagagattg caaagacatt aagggtgaag aggaaaagaa agaaggctgc gttaaac
//
LOCUS       DAMZII010000318         1276 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMZII010000318 DAMZII010000000
VERSION     DAMZII010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.324"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(77..502)
                     /locus_tag="POA77_004950"
     CDS             complement(77..502)
                     /locus_tag="POA77_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368248.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            696..1186
                     /locus_tag="POA77_004951"
                     /pseudo
     CDS             696..1186
                     /locus_tag="POA77_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          465 a          225 c          190 g          396 t
ORIGIN      
        1 tggcagtcta aagcatcaaa gttttgatca acatctttca ttttagacat ctccttgcaa
       61 tgtaaaatat catgtatcaa caacatctgg tgcaaatcca tgagtcgaac tcgacattca
      121 tcttagctcg attattattc cttcgtacag tcgatgtaaa caatacagaa agaggattat
      181 taagaaccag tttactcagt gaatcaatag aggaaaggac taacgtttct ttaaaagaat
      241 tgatttcatc atctgttaaa ctaaactcat cattgacaga tcgtgagata taactgcttt
      301 taactttact ctttacgttg actttattga cagaattaac attcacaaat cttgaattta
      361 atgcccatga tgttgtttgc gtgagaacct tctcagcatt aaggaatttt tttaacggaa
      421 tccttggttt ctttttcgag tccgaattta caatatcatg catatagacc atttcatgaa
      481 tctgcgaaaa ctcaatgtcc attatatacc tcacattttt accgtgactc gatgttactg
      541 ttcaataatc accttccatc aatactaaaa ttaatacccc taatgtgccg ataacaaata
      601 tagtcattct acgtaacgtc tccataaggt gatatttgac attatcagaa gctgcgaatt
      661 cggattttgc tctaatcaaa tgagagaaat gtaaaatgaa gccacgaaat attaataata
      721 gcctaccact gcaaccatta gttcctgatc aggagaacaa aaataagaaa aatgaagaga
      781 aatccgttaa tccagttaaa atcacaatgg ggtctggttt aaattatatt gaacaagaat
      841 ctcttggagg aaaatatcta acacatgatt tgtcaataaa gatagcggat atttctgaag
      901 agataattca gccagcaata ttatctgcta tgagcatata taaattttcg ataacagatg
      961 atttaatgag tatggctgta aatgaactca taaaactgac caaaatagag aataatgtag
     1021 acctgaataa attcactact atatgcacag acgttctatc cccccgagtc accagacata
     1081 ataaagaaaa aaaacaaacg acattctacc cttctcaaaa atcccctttt taattttcat
     1141 tgaaaaaatg gcaattgata ctatgaatcc aggtcataca cattaaggca tttatgtaat
     1201 gtcgtaatta agataactaa taaggtgaat attagtaaaa caactaatga tgttattagt
     1261 ttgtagtgaa cagtac
//
LOCUS       DAMZII010000319         1266 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMZII010000319 DAMZII010000000
VERSION     DAMZII010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.325"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            144..821
                     /locus_tag="POA77_004952"
     CDS             144..821
                     /locus_tag="POA77_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDI6368249.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            893..1177
                     /gene="ppnP"
                     /locus_tag="POA77_004953"
     CDS             893..1177
                     /gene="ppnP"
                     /locus_tag="POA77_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDI6368250.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          314 a          278 c          316 g          358 t
ORIGIN      
        1 ctggaatcat ttggaatctt tacattatgc cgtgcacgtc tgctgctacg ctttttgtca
       61 tttgtagcac aagtaagtgt cagcagtggt gcttcacact tgcccggtaa ttaacgacga
      121 aagaaaagta aggtggatga acaatgagtg cgtcgttggc gatcctgacc atcggcattg
      181 tacctatgca ggaggttttg ccgctcctga cggaatacat tgacgaagat aatatttccc
      241 atcatagcct gctggggaag ttaagtcgtg aagaagtgat ggcggagtac gcgccagaag
      301 caggcgaaga caccattctc acattattaa atgacaacca gctggcccat gtttcgcgtc
      361 gtaaagtgga gcgagacctg caaggtgtgg ttgaagtgct cgataatcag ggttatgacg
      421 tcattttatt aatgagtaca gcaaacatta gtagtatgac tgcgcgtaat acgatctttc
      481 ttgagccgtc gcgaatattg cctccactgg tttcctctat tgttgaagat catcaggtgg
      541 gggttatcgt tccggttgag gagatgctgc ccgttcaggc gcaaaaatgg caaattttgc
      601 agaaatcgcc ggtattttca ttgggtaacc ccattcatga ttcagaacaa aaaatcattg
      661 atgccgggaa agaattactg gcaaaagggg ctgatgtcat catgctggat tgtttaggat
      721 ttcaccaacg tcatcgcgat ttactgcaaa aacagctcga tgttcctgtc ttgctgtcta
      781 acgtattgat tgcacggctg gctgcggaat tactggtgta attttgcgtg acagtcagcg
      841 cctctggccc ctatagtgaa gtagatgttc aactaccaaa cagggccagt ttatgcttca
      901 aagtaatgag tacttttccg gcaaagtgaa atcaatcggc ttttccagca gcagcactgg
      961 tcgcgccagc gtgggtgtta tggttgaagg cgaatacacc ttcagcaccg ctgagccgga
     1021 agagatgacg gtaatcagtg gcgcgctgaa tgtgttactg cctgacgcga ccgactggca
     1081 agtgtatgaa gccggttcgg tgtttaatgt tcccggtcac agtgagtttc atctgcaagt
     1141 tgccgaaccc acctcttatc tgtgccgcta tctgtaattc ctcgccttcc ccttgaacgg
     1201 gagggcattt ttctgaaata tcctctcttt aacccataat aatatttcct ttgcttcgat
     1261 tttttc
//
LOCUS       DAMZII010000320         1260 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMZII010000320 DAMZII010000000
VERSION     DAMZII010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.326"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            126..884
                     /locus_tag="POA77_004954"
     CDS             126..884
                     /locus_tag="POA77_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDI6368251.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(937..1044)
                     /locus_tag="POA77_004955"
                     /pseudo
     CDS             complement(937..1044)
                     /locus_tag="POA77_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          319 a          287 c          293 g          361 t
ORIGIN      
        1 tacattgcta agttattcgc gcctttttat aattcgttac ctttccaggg acgctaatct
       61 ttgatcctta agaaagtttc cgggaccgcg atggtctctt tttttgttgt acggactctt
      121 gataaatgaa gcttttgaag acagttcctg ccatcgttat gttggcgggg ggcatgtttg
      181 cgtcactgaa tgcagctgcc gatgattccg tttttactgt catggacgat cctgccagcg
      241 ctaaaaaacc tttcgaaggt aatctgaacg caggatatct tgcacaatca ggcaatacga
      301 aaagctcctc acttacggct gacaccacca tgacctggta tggacaaacc actgcctggt
      361 cgctgtgggg gaacgccagt aatacttctt ctaatgatga gcgttcttca gaaaaatatg
      421 cggcgggcgg gcgtagccgt tttaatttaa ctgattatga ctatttattt ggtcaggcaa
      481 gctggctgac agaccgttat aacggctatc gagagcgcga tgtgttgacc gcgggttatg
      541 gtcgtcagtt tttgaatggc ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc
      601 gttacgacaa atataccgac aatgccagtg aaactcagcc gctgggctat gcctccggtg
      661 cttatgcatg gcagttgact gacaacgcta aatttacgca gggtgtttcc gtctttggcg
      721 cggaagacac aacgctgaac tccgaaagcg cattaaatgt tgctattaat gaacattttg
      781 gactgaaagt ggcttacaac gtcacatgga actctgaacc tccagagtct gcaccggaac
      841 ataccgatcg tcggacaacc ttatcgctgg gctacagcat gtaaaattat ctgccggacc
      901 gtgttatgcg gtctggctgt cttcgtttgt cactgctcag ccccaaatca ttgctgccca
      961 acgtagcaac aacatagcgc cacagattgc cagtagcacc accaccatct ttcagtgttt
     1021 tttatcaaag cgtttagtcg gcataccagt gtcctgcata tctcttgtgc gtgataacgg
     1081 agtgtaacaa aagggacagg catcaaggga ttttttgtta ttacagccgt cgggtggcgt
     1141 tgtacacctg atcactgttt ctgcgtttta tgaaaaaaat aaaattatgt atcgatatac
     1201 aaatatcata aatagctggt tataaatacg tttttagttc atcagcacta aaactcaggc
//
LOCUS       DAMZII010000321         1243 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMZII010000321 DAMZII010000000
VERSION     DAMZII010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.327"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            39..671
                     /locus_tag="POA77_004956"
                     /pseudo
     CDS             39..671
                     /locus_tag="POA77_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(716..1192)
                     /locus_tag="POA77_004957"
     CDS             complement(716..1192)
                     /locus_tag="POA77_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6368252.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMHIPLLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNQKKRQQYAPKYILYIPGLMN"
BASE COUNT          401 a          188 c          239 g          415 t
ORIGIN      
        1 aatgaatgcg ttaaagcatt tacactcagg atgaatatat gggaaaaatt aaaattgtag
       61 tttcagatca gcagccgttt atgattgatg ggataattgg atttctcgga cattatcctg
      121 atttatatga ggttgttggg ggctataaag atctgaagaa atctatagcg gagtgtaaca
      181 aatctacagc ctaaatattt attttaggag aatttgcagg tggaatgatg gggtccgaac
      241 tcgtaaaatg ggttaaatcg cataagatag atgctcatat tattacattt gtagcaaaga
      301 tgccgtatat tgattcaata aaattgcttg aagcaggtgc gaaaggatgc gtatggaaaa
      361 ccagtcaccc ggcgaaacta aatcgtgcta ttgattcgat tagtaatggt tacacttatt
      421 ttgatagtgt acatatggat tgtgaaaaaa tatcttccag gtattcctct gataatcaac
      481 ttacaaatcg tgagtcagaa atattacaac tcatagctga tggtaaaaca aacaaagaaa
      541 tcgctaattt cttgcagtta agcagaaaaa cagtcgaaac tcatagactc aatatcatga
      601 agaaattaga tgttcacagt ggtatcgagt tgatcaaaac agctttacgt atgggtgttt
      661 gcactatata aaaaaaagag gctttaggct cattttgctg aagccttacg attgtctagt
      721 tcattaagcc tggtatatac aatatatatt ttggggcata ttgctgtctc tttttttggt
      781 tatccattgc aaaacccgca ttataagtcc ccaggcattg ccacgttact ccacaacgtg
      841 aaaaatggtt atataaaatc tcggttccta ttttaatgtt cagacaaggg ttatctaaca
      901 aatctcgttc atcttttatt attccacgtt tttttaacag gggaatatgc atctggttta
      961 tctgcattaa ccaataatct aaactcttta tgttgttatt cttatctctg ttcttgccta
     1021 tgctatcctt ttttagattg ctttcaacaa gagcgatagc tttaattaaa tttggttcga
     1081 ttctgaacat tgttcctgct tcattagagc aagagttgct ggcgaaagac atttttgtaa
     1141 taaatataca aaacgtgaag atggttaaga atttaacttt tggtgagcgc atattttttc
     1201 atgtgtgagg attgaatgaa aggaacgaga tactattata aaa
//
LOCUS       DAMZII010000322         1233 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMZII010000322 DAMZII010000000
VERSION     DAMZII010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.328"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(108..>1233)
                     /locus_tag="POA77_004958"
     CDS             complement(108..>1233)
                     /locus_tag="POA77_004958"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HDI6368253.1"
                     /translation="FTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSN
                     YTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNS
                     MKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIV
                     PNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKN
                     LKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVI
                     PSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILK
                     TLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          388 a          246 c          208 g          391 t
ORIGIN      
        1 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
       61 aaaaacaaat aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
      121 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg ttcaaacaca
      181 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
      241 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
      301 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
      361 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
      421 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
      481 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
      541 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
      601 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
      661 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
      721 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
      781 taagattggt aatacttttt caagtataaa ctaggcacta tcattttaac attttgctca
      841 agcaattttg gttcaaatgc attatgcata tgtataacca ctcttgcctg aggagctcgc
      901 ttacgaattt gagtgtatag cttcatgcta ttatgaacta caataacact ttctttcgtt
      961 ataggaaaat catgtgcaat attcaatatt ctctgtgaat atggaagagg atcaagtctt
     1021 gtccattttt gaaataaccg cttatatatg cgactgaatc caaccctatg aatactacaa
     1081 ttatcattaa caaaggtgta atttgagtat cctggatttt tgatacaaac aatacgattt
     1141 ggaaaattgg tacgctgagc aacctgatac atccacgttt caacagcagc cgctccgcga
     1201 ggaggaattg aaaaaatagg agtaaccgta aat
//
LOCUS       DAMZII010000323         1193 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMZII010000323 DAMZII010000000
VERSION     DAMZII010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.329"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            93..878
                     /locus_tag="POA77_004959"
     CDS             93..878
                     /locus_tag="POA77_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6368254.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(1015..>1193)
                     /locus_tag="POA77_004960"
     CDS             complement(1015..>1193)
                     /locus_tag="POA77_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368255.1"
                     /translation="LIAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKC
                     NNEYISRKEDSAVK"
BASE COUNT          390 a          223 c          248 g          332 t
ORIGIN      
        1 tgtttgttat tatttaagtg gggtatataa ttagagtccg ttatcaatgc taaatattct
       61 aatcattatg acaggcgagg gagtgtccaa ttatgaattc aaaaaagctt tgttgcatat
      121 gtgtgttatt ctcgctgctt gcaggatgtg cctctgaatc ttctattgat gaaaagaaga
      181 aaaaagctca agtcacacaa agtaatatta ataaaaacac tccccagcaa ctgacagaca
      241 aagatttatt cggtaatgaa accactctgg ccgtatccga agaggatatt caagctgcgc
      301 ttgatggaga tgagttccgc gttcccctta attctcctgt aatacttgtt caatccggca
      361 accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa atattatact gtttccacat
      421 tctctggtat cccggacagg caaaagcctc tgacttgtaa caaaaacaaa gataaaaatg
      481 agaacgagga tgttgccagt gctgagaata tgaactggat gcaggcactg cgttttgtgg
      541 ctgcaaaagg acatcagaaa gcgattattg tttaccagga tacattgcag acaggaaaat
      601 atgactctgc gctgaaatca acagtatggt ccgactataa aaatgaaaaa ctcaccgacg
      661 ctatctccct gcgctacctg gtacgtttca cgctggtgga tgtggcaaca ggtgagtggg
      721 ctacctggtc gccggtgaat tatgaatata aagtgcttcc accattgccc gacaagaatg
      781 aagccagtac gactgatatg acagagcagc aaatcatgca acttaagcaa aagacctata
      841 aagcgatggt aaaagatttg gttaaccgct atcaataata aattatatct gccgccagga
      901 atttctggcg gcaataatac aaaattcttt gcataagaaa tttttcttga caaagacagt
      961 gcaagaaagg acatggaatc cggagttaaa aactgtttgt tttagaatat ttaattattt
     1021 aactgctgaa tcttccttgc gagaaatata ttcattatta catttcatgc catttttaat
     1081 atagattgct cctgggaaaa tccccgtatt attaccgcag gggtgataat tagcattgac
     1141 atatcctgtg acaaaaggag ctattaaagg tgctattacg atagctatta gta
//
LOCUS       DAMZII010000324         1180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMZII010000324 DAMZII010000000
VERSION     DAMZII010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.330"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            242..>1180
                     /gene="leuO"
                     /locus_tag="POA77_004961"
     CDS             242..>1180
                     /gene="leuO"
                     /locus_tag="POA77_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDI6368256.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICK"
BASE COUNT          316 a          241 c          269 g          354 t
ORIGIN      
        1 tcgcggtagt tatgattaga ttgttttcgc aacaaaaaca ttatggatta ttatgctgtg
       61 gtaaatgact cattccacgg caatggattc tgtttttatc agaacccgta tctttatgtt
      121 ttccgaattt tactcatttt gctttttctt attttatatg catgataaat catattcttc
      181 aggattattt ctctgcattc caataaggga aagggagtta agtgtgacag tggagttaag
      241 tatgccagag gtacaaacag atcattcaga gacggcggag ttaagcaagc cacagctacg
      301 catggtcgat ctcaacttat taaccgtttt cgatgccgtg atgcaggagc aaaacattac
      361 ccgtgccgct catgttctgg gtatgtcgca acctgcggtc agtaacgctg ttgcacgcct
      421 gaaggtgatg tttaatgacg agctttttgt tcgttatggc cgtggtattc aaccgactgc
      481 tcgcgcattt caactttttg gctcagttcg tcaggcattg caactagtac aaaatgaatt
      541 gcctggttca ggttttgaac ccgcgagcag tgaacgtgta tttcatcttt gtgtttgcag
      601 cccgttagac agcattctga cctcgcagat ttataatcac attgagcaga ttgcgccaaa
      661 tatacatgtt atgttcaagt cttcattaaa tcagaacact gaacatcagc tgcgttatca
      721 ggaaacggag tttgtgatta gttatgaaga cttccatcgt cctgaattta ccagcgtgcc
      781 attatttaaa gatgaaatgg tgttggtagc cagcaaaaat catccaacaa ttaagggccc
      841 gttactgaaa catgatgttt ataacgaaca acatgcggcg gtttcgctcg atcgtttcgc
      901 gtcatttagt caaccttggt atgacacggt agataagcaa gccagtatcg cgtatcaggg
      961 catggcaatg atgagcgtac ttagcgtggt gtcgcaaacg catttggtcg ctattgcgcc
     1021 gcgttggctg gctgaagagt tcgctgaatc cttagaatta caggtattac cgctgccgtt
     1081 aaaacaaaac agcagaacct gttatctctc ctggcatgaa gctgccgggc gcgataaagg
     1141 ccatcagtgg atggaagaac aattagtctc aatttgcaaa
//
LOCUS       DAMZII010000325         1163 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMZII010000325 DAMZII010000000
VERSION     DAMZII010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.331"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..523)
                     /gene="sctT"
                     /locus_tag="POA77_004962"
     CDS             complement(<1..523)
                     /gene="sctT"
                     /locus_tag="POA77_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDI6368257.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYI"
     gene            complement(525..785)
                     /locus_tag="POA77_004963"
     CDS             complement(525..785)
                     /locus_tag="POA77_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6368258.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(795..>1163)
                     /locus_tag="POA77_004964"
     CDS             complement(795..>1163)
                     /locus_tag="POA77_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6368259.1"
                     /translation="KSYLIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLP
                     AYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVIISTPIKLILFVAMD
                     GWTMLSKGLILQYFDLSINP"
BASE COUNT          418 a          236 c          189 g          320 t
ORIGIN      
        1 taatatatga gaataaaggc tctaatttag gaaaattaaa attgcctgaa ggccataaat
       61 tataactttg ccaaagagtt tccagaataa agttcaatcc accgttagta agataaaccg
      121 ctgcagagaa taggttaaat aatcttgcta attccgaagt atcgacacca gtagcaggat
      181 ccaaagtact acttaacgtt gcaccacgtt ggttatcgat aaaactacct atagcaagga
      241 aaatccaaaa cggggatgat aataagcaag caatgaacag ccctatgata atttctctgg
      301 cagcaaacaa agcgatatcg atttcattta aggtcgataa ttcatagtgg taatgctgaa
      361 ataatgcaca tgataccact attattattg gatgacggac tactgttggt atattcccac
      421 tggcgaaaaa tggtaacaaa tagaaagttg gtgccaatcg gcaaaaaccg agcgctgtag
      481 cagccagcag actatgcaac tgatagagta tcgcctcctc catcctatcc tctcgcgaat
      541 gctaaattca gcatttcgat accaaacgaa tataattttt caccatacca tccgctcatt
      601 aaaaaaaagc aaatacttac gcataataat ttgacgccaa atggcaacgt ctgttcttgt
      661 aactgtgtta ccgtttgaaa aagccctacc aataaaccaa caaatgtcgc aactgcgata
      721 ggtcctgcag acataactaa aatcagatac agcgccctat tccctgcaaa aacaatatcg
      781 ttcatatttt ttcctcaggg attaatggat aggtcaaaat attgtaaaat gagtcctttt
      841 gataacattg tccaaccgtc catagcgaca aatagaatta attttatcgg tgtagatatg
      901 attaccgggc tcatcatcat catacctaac gttaacagaa cgctggatat aaccagatcc
      961 actacgacaa agggtaaata gatataaaaa cctataataa atgcactttt tatctcactc
     1021 aatgcatatg cgggcagtaa cgagaaaatt gaaatattat catcatcgat tatctcttca
     1081 ttgtcttcag aactattcac cttctgaatc ttttcaaaaa agctaaccaa ctcaggttca
     1141 gaatatttta tcaaatatga ttt
//
LOCUS       DAMZII010000326         1154 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMZII010000326 DAMZII010000000
VERSION     DAMZII010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.332"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            135..375
                     /locus_tag="POA77_004965"
                     /pseudo
     CDS             135..375
                     /locus_tag="POA77_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            395..682
                     /gene="sctI"
                     /locus_tag="POA77_004966"
     CDS             395..682
                     /gene="sctI"
                     /locus_tag="POA77_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDI6368260.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKEGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            723..>1154
                     /gene="sctJ"
                     /locus_tag="POA77_004967"
     CDS             723..>1154
                     /gene="sctJ"
                     /locus_tag="POA77_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HDI6368261.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPI"
BASE COUNT          381 a          199 c          241 g          333 t
ORIGIN      
        1 taaacaatat gtaagattta atgtgaattt gaaaaatcag gacacaaata atagttcatt
       61 tagctacgga gataaccgat tcgagaagtc tcaaggtagc aactggacat ttcaggaata
      121 attgaggagg acttatggca gattggaatg gttatattat ggatatcagc aatcaatttg
      181 atcagggcgt tgatgatctg aaccagcaag ttgaaaaagc gttggaggat ttagccaacc
      241 aatccctccg acacgaaatt tcttgccgaa tatcaaagtg cattagctga gtatacatta
      301 tatcgaaatg cgcaatctaa cgttgtaaaa gcgtataaag atcttgattc agcaatcata
      361 caaaacttcc gctaaactgg ataaggattt tattatgtca gtaagcaata tgccacctat
      421 tgatagagct gagcaatcaa ctgctcatga aatacagcaa gcgaaagtga ttgatttaaa
      481 tgaccgggtt ctcaatcttg acaacccgga tgataaaatg atctccgctt ttgccaacta
      541 tgctgtacaa actgaaaatt ggcagcaaaa tgcgttacaa gcattaaggt ctgataaaga
      601 ggggttaact cctgaaaaat tattagtctt gcaggatcat gtgcttaatt ataatgtcga
      661 ggtttcattg gtggaacatt agcaagaaag attgtcgctg cagttgagac gttgacccgc
      721 tcatgaaata tatatcatta ttattgttta tcctgctgtt gtgcggctgc aagcagcagg
      781 aattattgaa ccatcttgat caacagcaag ctaatgatgt ccttgctgtt ttacaaagac
      841 ataatattaa tgctgaaaag aaggatcaag gcaaaacagg attttcaata tttgtcgaac
      901 caacggattt tgcttctgcg gtggattggc taaaaatcta caatctaccg ggtaagcctg
      961 atattcaaat atcgcagatg ttccctgcag atgcactcgt ttcatctcca cgagcggaaa
     1021 aagcaaggct atattctgct attgagcagc ggttagagca atctctcaaa attatggatg
     1081 gcattatttc ttcgagagta catgttagtt acgatgttga taatggcgat agtggtaaaa
     1141 ctgctttacc aatt
//
LOCUS       DAMZII010000327         1142 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMZII010000327 DAMZII010000000
VERSION     DAMZII010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.333"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1142
                     /locus_tag="POA77_004968"
     rRNA            <1..>1142
                     /locus_tag="POA77_004968"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          300 a          251 c          356 g          235 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagtccctc
      481 gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      601 ttaattgatg gggttagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg
      661 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa
      721 tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga
      781 agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca
      841 ctgaacattg agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc
      901 tgcatggagc cgaccttgaa ataccaccct ttaatgtttg atgttctaac gtggacccgt
      961 aatccgggtt gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga
     1021 gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg
     1081 gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt
     1141 ga
//
LOCUS       DAMZII010000328         1134 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMZII010000328 DAMZII010000000
VERSION     DAMZII010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.334"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1134
                     /gene="espL4"
                     /locus_tag="POA77_004969"
     CDS             <1..>1134
                     /gene="espL4"
                     /locus_tag="POA77_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDI6368262.1"
                     /translation="RIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELATPL
                     LKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEE
                     YLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYI
                     NDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRYVAACGDFGLVMK
                     AYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRIM
                     SESKEDIKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDEITP
                     PLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSG"
BASE COUNT          399 a          222 c          203 g          310 t
ORIGIN      
        1 tcgtatccct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatggtc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgata attaataaaa acgaaaacac gacacttact caaaccattg ccccaatatt
      301 tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      361 aaacctaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      421 ttattttccg gggcgacccc aaaaccgtat agtatgccga cacattgctg cacaatatat
      481 taatgatatt tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga
      541 aaaatttctc acgcacttca acaaaaaatg caaaaaccag actttggcgt tggtttccag
      601 ccgtcctgag gggcgttacg ttgctgcctg tggtgatttc gggctagtta tgaaggcata
      661 tttcgacaag atggaatcaa atggcatcag tgttatggca gccatattat tggtggataa
      721 ccatgctttg acggcgcggc taagaataaa gaacacaact gaaggatgta cccattacgt
      781 ggtttcggtt tatgatccta atgtcactaa cgataaaata agaattatga gcgaaagcaa
      841 agaggatatt aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa
      901 atggtcaaat gatcatatta ttaaccaatc tgttgcaata attccagcac ttccgaaaga
      961 acagctattg atgttaaaag gatctgtgga tgaaataacc cctccattat cacctgcaac
     1021 gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcaact
     1081 ccagaaaatg ccagaactac atagaacaga aatgttgact gcctataata gtgg
//
LOCUS       DAMZII010000329         1128 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMZII010000329 DAMZII010000000
VERSION     DAMZII010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.335"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            4..>1128
                     /locus_tag="POA77_004970"
     CDS             join(4..253,253..>1128)
                     /locus_tag="POA77_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
                     /protein_id="HDI6368263.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAASAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATQRGYQMGRWLAGRLMKELGLVSCQQPTYRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESE"
BASE COUNT          312 a          275 c          315 g          226 t
ORIGIN      
        1 ataatgaaaa aaagaaattt cagcgcagag tttaaacgcg aatccgctca actggtcgtt
       61 gaccagaatt acaccgtggc agatgcagcc agcgctatgg atgtcggcct ttccacaatg
      121 acgcgatggg tgaaacaatt acgtgatgag cggcagggaa aaacaccaaa agcctcccct
      181 attaccccgg aacaaattga aatccgtgag ctcaggaaaa agctacaacg tattgaaatg
      241 gaaaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt cagactccct gaacagttct
      301 cgataatcgg gaaactcagg gcgcgttatc ctgtggccac tctctgccat gtgttcgggg
      361 tccatcgcag cagctacaaa tactggaaaa accgtcctga aaagccagac ggcagacggg
      421 ctgtattacg cagccaggta cttgaactgc atggcatcag ccacggctct gccggagcaa
      481 gaagcatcgc cacaatggca acccagagag gctaccagat ggggcgctgg cttgctggca
      541 gactcatgaa agagctgggg ctggtcagct gtcagcagcc gacttaccgg tataaacgtg
      601 gtggtcatga acatgttgct atccctaact accttgaacg gcagttcgcc gtgaccgagc
      661 caaatcaggt gtggtgcggt gatgtgacct atatctggac gggtaagcgc tgggcgtacc
      721 tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc
      781 cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt ggtaagcccg
      841 tcggggtgat gttccacagc gatcaaggca gtcattatac gagcaggcag ttccggcagt
      901 tactgtggag ataccggatc aggcagagta tgagtcggcg tggaaactgc tgggataaca
      961 gcccaatgga gcgcttcttc aggagtctga agaacgaatg ggtgccggcg acgggctatg
     1021 taagcttcag cgatgcagct cacgcaataa cggactatat cgttggatat tacagcgcac
     1081 taagaccgca cgaatataat ggtgggttac caccaaacga atcggaaa
//
LOCUS       DAMZII010000330         1122 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMZII010000330 DAMZII010000000
VERSION     DAMZII010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.336"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(82..363)
                     /locus_tag="POA77_004971"
     CDS             complement(82..363)
                     /locus_tag="POA77_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368264.1"
                     /translation="MKLSQLEVGMTVWSLSRTKMGNTTIKTVTLHSVVIKEVHDNHVI
                     ASWNGNAPRRFGETAITGWKKEKPLLIRDRSGSARLATREEKARILDTK"
     gene            complement(495..665)
                     /locus_tag="POA77_004972"
     CDS             complement(495..665)
                     /locus_tag="POA77_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368265.1"
                     /translation="MKPFGLAGKPQPEPCGCCWYHPEDCRYAAAKTLYRRSQRKAERQ
                     RQRASITRALAE"
     gene            complement(707..1030)
                     /locus_tag="POA77_004973"
     CDS             complement(707..1030)
                     /locus_tag="POA77_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368266.1"
                     /translation="MNKPLVSFAELSGNAINVARQSVIDMEMDATREKIGKARSLFHS
                     GIHRAVNGYPLIQSAANQLAVIKRLLGDTKYLDACITENLCMFSPEGYLYLFMQRRFI
                     NEPVA"
BASE COUNT          274 a          287 c          288 g          273 t
ORIGIN      
        1 gatgcagtaa gcggctttgg ttccctccgg ttctgtgggg aggaaacggc cagaacagca
       61 gaccgatacg atgggcgggc gctattttgt atccagaatt cgtgcctttt cttcccgcgt
      121 cgccagtcgg gcacttccgg aacggtcgcg gatcagcagt ggcttttcct ttttccaacc
      181 agtgatagcc gtttccccga aacgacgcgg tgcattgcca ttccagctgg caatcacgtg
      241 attatcgtgt acttccttga tgacgacaga atgaagagtc accgtcttaa tggtggtgtt
      301 tcccattttg gtccgggaga gtgaccagac ggtcatcccc acctccagct gactgagttt
      361 catctttact ccggtaatat cgggatacat cattaaacga cgtggagacg taggttagca
      421 taagcactca cagcggatac tgccgccaga taacagggtg aggtatccgc catgagcagt
      481 ctttacggct ggcgttattc ggccagggcg cgggtaatag acgcacgctg acgctggcgc
      541 tcagccttac gctggctgcg gcgatatagc gtcttcgcgg cagcgtagcg acaatcttcc
      601 gggtggtacc agcaacagcc gcagggctca ggctggggtt ttccggcaag tccgaatggt
      661 ttcataatgt caccttttaa atggaataat gttcaggtcc cgtccatcag gccacgggtt
      721 cattaatgaa gcggcgttgc ataaacaggt acagataacc ctctggcgag aacatacaaa
      781 gattctcggt aatacaggca tcgaggtact tcgtgtcgcc aaggagtcgc ttaatgacag
      841 ccagctggtt agccgcagac tgaatcagcg gatagccatt cacggcacga tgaatacctg
      901 agtggaataa cgaacgagcc tttccaatct tttcccgggt tgcgtccatt tccatatcga
      961 taacagactg acgcgcaaca ttaatagcat tgccggataa ctccgcgaag ctaaccagag
     1021 gcttattcat aaaaaactcc ttccaggtat gtgagcttat tatatcaata cggactttgc
     1081 cccaaacaac ccgactcgaa atattttgga aattactata at
//
LOCUS       DAMZII010000331         1122 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMZII010000331 DAMZII010000000
VERSION     DAMZII010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.337"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(51..>1122)
                     /locus_tag="POA77_004974"
     CDS             complement(51..>1122)
                     /locus_tag="POA77_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6368267.1"
                     /translation="GGAVLDKLLDNCNNINLLLLVRAPTPQAGLERIKENMRKFNVCE
                     ERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGNNPFIWNVNVTGTL
                     AFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHLVEYTHSKATIEYL
                     MRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKFMCSLDDKIDVIPV
                     DYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALNCVPVGDRYTKVSY
                     DILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLLSIGMPKPPKFTDY
                     IKYCIETTKHLSIQQQMEVDFK"
BASE COUNT          362 a          238 c          188 g          334 t
ORIGIN      
        1 tttatatatt atgagtgcaa caccaacaat aattctgatt atgataaata ttatttaaaa
       61 tcaacttcca tttgttgttg aattgaaagg tgttttgtcg tttctataca gtatttaata
      121 taatcagtaa actttggcgg tttaggcatt ccgatactca gtagcttgtc gttactgaaa
      181 caaacattga gcatactgaa cgctccatat aaacgaatgg cttttaacat aaggcgttcg
      241 ttacagggac caaaaatatt tttaaagtca tgacggctca ttgccagtat gtcataactg
      301 actttagtat atctgtctcc aacaggaaca cagttcaaag cacgggctac agcttcgtca
      361 atagcagaga acgtcacact actttcttta cctgctgata tatgaacaat ctcaccatta
      421 attaacgagc tttcaagcaa cattagcaat gcatcagcac aataatctac agggataaca
      481 tctattttat catccagaga gcacataaat ttttgcaaca ttaaccccat tctgaatacc
      541 cagaaaatac tggttgaagg taagcaccct aaacgactgt ggccaacaat aattgatggt
      601 cgggcaacca acaaaggtaa atcaggacac tgcttacgca tcagatattc tattgttgct
      661 tttgaatgcg tatactccac taaatgttca cctgtttctg atgaagcaga ctcttcctta
      721 actagcgacc ccgtatgagg tgtacaagac atagcagtac caacatgaag gaagcgtttc
      781 agtcctgcca cttttgccat tcttcttgca aaagcaagtg tacctgtaac attcacattc
      841 catataaaag gattattacc aaaagaagct atagccgcac agtttataac atgagtgact
      901 tcatcaagac gaggatccat gagaaaggct tccggattat ttagatcccc aggcaagatg
      961 ttatcattag ttaatgcatg caacctttcc tcacaaacat taaatttacg catattttct
     1021 ttaattcttt ccagtcccgc ttgtggagta ggtgctcgta ctaaaagtag caaatttata
     1081 ttattacagt tatccagcag cttatccagg accgcgccac ca
//
LOCUS       DAMZII010000332         1114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMZII010000332 DAMZII010000000
VERSION     DAMZII010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.338"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(171..>1114)
                     /locus_tag="POA77_004975"
     CDS             complement(171..>1114)
                     /locus_tag="POA77_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HDI6368268.1"
                     /translation="LKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGKAK
                     ERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMVDR
                     DWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYSFT
                     AKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYLYDSFEFGGSFKY
                     SGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRITN
                     KKGDTSLYSRNLNIYTKNGAGIESYNFMTTAGLKYYF"
BASE COUNT          322 a          257 c          205 g          330 t
ORIGIN      
        1 ggcagggcag catcgttaaa aatacaagag ttaacagaaa cacttttatc gtggcgcaca
       61 tatatcctcc acatattata aaaaataaaa agaccaccat cagaagtgct ggccttttgt
      121 ttatcatatt taaagtgtca tgaaaaaatt aaaaacaggc atgtttcagg ttaaaaataa
      181 tacttcagac cagctgtggt cataaagtta tagctttcaa tgcctgcacc atttttggtg
      241 tatatattca aatttctgga gtacagagaa gtatcacctt tcttattagt aatacggttc
      301 catgttcctt cgacataaac tttagctgca ggtgtaatat aataaccagc atgtagtgag
      361 acagagtaat agttctgatt atttacatcg ctacgatagg tgatcctttt ctcagggttg
      421 taatgctcat cattatccga tgccttcacc catccgctat atttgaaact cccaccgaac
      481 tcaaagctat catacagata attaccggtt aaccctatat agggcatttt aaagtgttgt
      541 ttgtagccta tagctctttc accatctgga aatgatcccg tttcatcccg gaagcctccc
      601 tcactactat agatataaga gcctcctttt gctgtaaagc tataacgatt ttcctggtaa
      661 ccagccataa gacctaactg atagtctggt tgattcagga gccatccctt aatattcagg
      721 tcgaattcat tagcaaaatt tagacgggta ttcggatgtt tactctcatc agtccaggtt
      781 ccgggattgc tcgtatccag ccagtccctg tccaccatat tgcctccacg accagctaat
      841 gttgtccacc ctgaggcacc gacagatact ctgggtaaca gatcccagtt aaatgccccc
      901 ttgacaattg gcgcattact gtatttccag tccagctggc tggctttgcg ccctttttct
      961 tcaggcagat aaacccgttc tttggccttg ccacttaaag tgccaaaatc aatctccgta
     1021 ctgattttct ccggtgtaaa agaaagccca gtatcagaag ccagtgcagc aaacgctaca
     1081 ggagctgaga gtgcagtagc aagaatcttt aagt
//
LOCUS       DAMZII010000333         1104 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMZII010000333 DAMZII010000000
VERSION     DAMZII010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.339"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1015
                     /locus_tag="POA77_004976"
     CDS             <1..1015
                     /locus_tag="POA77_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin-B"
                     /protein_id="HDI6368269.1"
                     /translation="MQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYLGDKYKALSHEIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSLTLGSALIAFGLSATVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
     gene            complement(1033..>1104)
                     /gene="cui"
                     /locus_tag="POA77_004977"
     CDS             complement(1033..>1104)
                     /gene="cui"
                     /locus_tag="POA77_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HDI6368270.1"
                     /translation="VLTMTYAILLMPLLVIKYFKGRQ"
BASE COUNT          337 a          189 c          273 g          305 t
ORIGIN      
        1 tatgcagaat ggtacacaat atatggctat tatcggaggt attccgatga cagtgccagt
       61 agtggatgcc gttccagttc cggaccggag tcgtccggga accaatatta aagatgttta
      121 cagtgcccct gtatcaccaa atctaccgga cctggtatta agtgtgggtc agatgaatac
      181 tccagttcgg tctaatcccg aaatccagga agatggcgtt atttctgaga cagggaatta
      241 tgttgaggct ggttatacga tgtccagtaa taatcatgat gtcattgtcc gttttcctga
      301 aggcagtgga gtttctccgc tatatatttc agccgtggag attctggaca gtaatagttt
      361 aagccagcgc caggaagccg aaaataacgc aaaggatgac ttcagagtca agaaagaaca
      421 agaaaatgac gagaagacgg tcctgacaaa aaccagcgag gtcatcatta gtgtcggtga
      481 caaagtcggg gaatatcttg gagataaata caaggcgctt tcccatgaaa ttgcagagaa
      541 tataaataat tttcagggaa aaacgattcg tagttatgat gatgcaatgt cttccattaa
      601 taagttaatg gctaacccca gccttaaaat aaatgcaacg gacaaagaag ccattgtgaa
      661 tgcgtggaaa gcatttaatg ctgaggatat ggggaataaa tttgctgcgt tgggtaaaac
      721 gttcaaagca gcagattatg caataaaggc aaacaacatc agggagaaga gtattgaggg
      781 ttaccagact ggtaactggg ggccattaat gctggaagtc gagtcctggg ttatcagtgg
      841 gatggcatct gctgtagctc ttagtttgtt ttctttgaca ttaggctcgg cccttatagc
      901 ctttggtctt tcggccacag ttgttggttt tgttggcgta gttattgcag gtgctattgg
      961 tgcatttatc gatgataaat ttgttgatga gttgaatcac aagatcataa aataattaaa
     1021 aggaaggcag tgttactgcc tccctttaaa atatttaatg acaagtaatg gcatcagtaa
     1081 aatcgcatat gtcatggtta aaac
//
LOCUS       DAMZII010000334         1060 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMZII010000334 DAMZII010000000
VERSION     DAMZII010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.340"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..806
                     /locus_tag="POA77_004978"
     CDS             <1..806
                     /locus_tag="POA77_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368271.1"
                     /translation="SVDTLTFKYQSIIKGLDRLFEGINLDSAFSLSEEERSYFEEKAK
                     SLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIR
                     FLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRI
                     YPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRA
                     NGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            808..>1060
                     /locus_tag="POA77_004979"
     CDS             808..>1060
                     /locus_tag="POA77_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368272.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRI"
BASE COUNT          348 a          172 c          201 g          339 t
ORIGIN      
        1 agtcagttga taccctaact ttcaaatatc aatcaataat aaaaggtctc gatagattat
       61 ttgaaggtat aaacttagat tcagcattct cattatctga agaagaacgc tcatattttg
      121 aagaaaaagc taaaagttta aacgcgcttg cacagttagg tattagtgtt gagataatat
      181 ctcatgaact tgaagaaatg gattctatgg taaccagagg actaaactct cttcctactt
      241 ctgtaaaaga acaccctggt ttttcattgg cgttaaatgc tcacagatcg cttactcaac
      301 aaatacgttt cttatcacct ttgaaaatat caggttatca atccaggcag agaataactg
      361 gaaaaaatat catggattat gtcctgaagt tctttgggga gcgtttcgaa cggcaacgaa
      421 taactattga atttagtgaa gagtttaagc aaatcgcaat aacagatata ccatcaagga
      481 tctatcctgt ttttactaat attatcaaca atgcaatgta ttgggtcagt ctgtcaaata
      541 ataggctcat aaagattggt tttgtgaatt ctttggttat catagcaaat tctggtccgg
      601 caattgatac cgatgatatc ccgcgactat ttgaactatt ttatagcaaa agagcaaatg
      661 gacatggggt aggtctgtat ctatgtcgag aaaaccttgc tgttgcacat cataaaatat
      721 ggtattcaga acctgatgaa ggcgataact atttaataaa agatggcgct aattttgtga
      781 tccagttcaa tggagtggag ttctaatatg acagtggcaa attataattc tcttgtccag
      841 aaaactttct gcgaaaatgc aattcgttcc gttgtcatga ttgatgacga ttttctgacg
      901 tattctgaat caatcagggc gttgaataac gaagttgatt tagactacaa caaaattgac
      961 tcatctaaac gagccgctac tcttgagagc ttttttcaat ctaaaaatat gatttgtgat
     1021 gttgacaatg gttctgttaa tttcgatgtg gatcggatta
//
LOCUS       DAMZII010000335         1055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMZII010000335 DAMZII010000000
VERSION     DAMZII010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.341"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            187..819
                     /locus_tag="POA77_004980"
     CDS             187..819
                     /locus_tag="POA77_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6368273.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          360 a          179 c          189 g          327 t
ORIGIN      
        1 gattaaatca gtgaaacata atcatattta ataacgagaa atgcatttta aaatgtctta
       61 atcagaatta tagcaaatac agattaatcg caatttttcc cagcaaaatt acaaaatcat
      121 ggtgtattga catcatcaat caccaatgaa attatgcaat tatatacgga tagggaggtt
      181 cttaacatgg ggctttgtag tcgttataaa agtcttacat gcaatagttg ctcaatgcat
      241 tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact gcgcaaactc gtgtttttgt
      301 atgtggcccg aagaaagttc atattttaat cgtggtgtgg tagaaggtat cttaacaaaa
      361 aaccacaatg cgagattaag cggatatatt tttgtcgatt tttcagtaag ttttttacgt
      421 ctattccttg aaaaggactg gattgactat cttgcaagta ctgacatggg gattgtttta
      481 gtcagcgacc gcaatatgca atcgttagct aattactggc gaaaacacaa ctcagcaata
      541 tctgctgtca tatataatga tgatggtctt gatgttgcca atgaaaagat cagacaacta
      601 tttattggtc gttatttatc atttaccaga ggtaacacat taactcagat ggaattcacc
      661 ataatggggt atatggtttc tggttataat ccatatcaga ttgctgaagt tctggatatg
      721 gatatccgta gcatctacgc gtacaagcaa cgaatcgaaa agagaatggg tggtaaaata
      781 aacgaattat ttattcgttc acattcggtc caacattgat actcaacgac cagccagaat
      841 catactctgt ttatacgggc atttattgaa atgtccgtat tttataaata ggccgtcccc
      901 tcaagagtaa acaccatagt atttaaaacc atcacacgaa aaattcagaa gcattacgaa
      961 atatgaattt acaaacaata gtggcataaa tgttaaccat gttaatttac gtaaagtttt
     1021 acgttgcaac attaaagcct catttcaatc atcat
//
LOCUS       DAMZII010000336         1025 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMZII010000336 DAMZII010000000
VERSION     DAMZII010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.342"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..213)
                     /locus_tag="POA77_004981"
     CDS             complement(<1..213)
                     /locus_tag="POA77_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368274.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTC"
     gene            complement(920..>1025)
                     /locus_tag="POA77_004982"
     CDS             complement(920..>1025)
                     /locus_tag="POA77_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6368275.1"
                     /translation="MEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          296 a          221 c          177 g          331 t
ORIGIN      
        1 gcatgtatag gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga
       61 tgtgagaaat tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc
      121 ggccttacag gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga
      181 tattagtgcc cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc
      241 agtaactgca ctcgcagaaa tagagaaaaa aatacaattg ttaataacct tgccatattt
      301 tttagtccgt aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt
      361 gcatgtcatt agataaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg
      421 gaccaaaagc gtttaaacaa ctatgtattg aaaatccatt gcgattataa gttcctgtat
      481 taactgacgg tactaatcgt atgccgtcag gttttctatt gcttgctccc gggtcattaa
      541 cattctaact ccataggcct tgaggtattg gccatctatt tataaatgtg tacaacgggg
      601 attattttta cattgcatat tattaccaga agcgactgga atactaagca catcataaaa
      661 attatcatca catggtatat ctgaaacaca cgaaagcata cctaaagtta tactgtataa
      721 acaggtggct aatcccaagg ggatcgattc cagaaatcgg gttcaacgga tactgataaa
      781 aattccacct accttccagc ccaatcggag cctgagtgtt ataccgcccc tgctgcggtt
      841 aatgttaccg ataatagtta tagtacagcc ctgattcatc atcatactac tgctgcggca
      901 gtctgatgaa ctattgcttt tacggattct ccttattctt ccttctatcc attcgcttta
      961 gctttttgag ctcttgaagt ttgctctttg catcaatatt accgttattt acagcgaatt
     1021 ccatc
//
LOCUS       DAMZII010000337          916 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMZII010000337 DAMZII010000000
VERSION     DAMZII010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.344"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(45..371)
                     /locus_tag="POA77_004983"
     CDS             complement(45..371)
                     /locus_tag="POA77_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368276.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..790)
                     /locus_tag="POA77_004984"
     CDS             complement(371..790)
                     /locus_tag="POA77_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368277.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          298 a          205 c          133 g          280 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatata
//
LOCUS       DAMZII010000338          899 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMZII010000338 DAMZII010000000
VERSION     DAMZII010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.345"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..636
                     /gene="aroL"
                     /locus_tag="POA77_004985"
     CDS             112..636
                     /gene="aroL"
                     /locus_tag="POA77_004985"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDI6368278.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            686..877
                     /gene="yaiA"
                     /locus_tag="POA77_004986"
     CDS             686..877
                     /gene="yaiA"
                     /locus_tag="POA77_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDI6368279.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          250 a          211 c          234 g          204 t
ORIGIN      
        1 tttaagtgga attttttctt tacaatcgaa attgtactag tttgatggta tgatcgctat
       61 tctcatgaca ccggctttcg ccgcattgcg acctattggg gaaaacccac gatgacacaa
      121 cctctttttc tgatcgggcc tcggggctgt ggtaaaacaa cggtcggaat ggcccttgcc
      181 gattcgctta accgtcggtt tgtcgatacc gatcagtggt tgcaatcaca gctcaatatg
      241 acggtcgcgg agatcgtcga aagggaagag tgggcgggat ttcgcgccag agaaacggcg
      301 gcgctggaag cggtaactgc gccatccacc gttatcgcta caggcggcgg cattattctg
      361 acggaattta atcgtcactt tatgcaaaat aacgggatcg tggtttattt gtgtgcgcca
      421 gtatcagtcc tggttaaccg actgcaagct gcaccggaag aagatttacg gccaacctta
      481 acgggaaaac cgctgagcga agaagttcag gaagtgctgg aagaacgcga tgcgctatat
      541 cgcgaagttg cgcatattat catcgacgca acaaacgaac ccagccaggt gatttctgaa
      601 attcgtagcg ccctggcaca gacgatcaat tgttgatttt agagcgccta tacttaacgt
      661 tcatcccgtg aaataaggaa gaacgatgcc aacgaaaccg ccttatcctc gtgaagcata
      721 tatagtgacg attgaaaaag gaaagccagg acagacggta acctggtacc aactcagagc
      781 cgatcatcct aaaccagact cgttgatcag tgaacatccg acggctcagg aagcgatgga
      841 tgcgaaaaaa cgctatgagg accctgacaa agagtgaccg catcagactg ctcggaagg
//
LOCUS       DAMZII010000339          888 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMZII010000339 DAMZII010000000
VERSION     DAMZII010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.346"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>888)
                     /locus_tag="POA77_004987"
     CDS             complement(<1..>888)
                     /locus_tag="POA77_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDI6368280.1"
                     /translation="CSVCPGGMTSGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYS
                     SYRTKTPAPVGIFGPGWKAPSDIRLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSE
                     SLWLVRGGKATQPDGHTLARLWASLPPDIRLSPHLYLATNSAQGPWWILGWSELVPGA
                     EDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQA
                     QRAEEARKQRTASLSSPDTPRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPE
                     SLPGAPLARYTYTEAGELLA"
BASE COUNT          151 a          306 c          289 g          142 t
ORIGIN      
        1 cgccagcagt tcaccggctt ccgtatacgt gtaccgcgcc agtggcgcac cgggcaggct
       61 ttccgggtat gccgggtcgt gcatcagcca caccgccgaa agacggatgc ccctgtcggg
      121 gccgtattcg gtaccgggca gtgtgtcggg gaacgctgag gctgagagag ggcggggtgt
      181 gtcaggggaa gataaggaag cggtgcgctg tttacgggcc tcttccgcac gctgcgcctg
      241 cgtggtcagc accagacgga actcccgccc ggcaccgtcc gtcacgccgg tgatttcccc
      301 ggccaggtca ccggcggcct cacgccggta cgtcagcgtc cgcccgaagc ggtctgccag
      361 cccggtaagc acccggtacg gcggcagcgg cgctggcagt acgtcctcag cacccggcac
      421 cagctctgac caccccagta tccaccacgg cccctgtgcg ctgttggtcg ccaggtaaag
      481 atgcgggctt aaccggatat ccggcggcag cgacgcccac agccgtgcca gcgtatggcc
      541 gtccggctgc gttgccttac cgccacgcac cagccacagg gactcgctgc ggctgtacac
      601 cgcctcaccc ggcagcagcg gctcaaagtg aatgctccgt ccgccgttgt cgttgagtac
      661 cagtgcatca tcgcgtatct gtaagcggat atcagaaggc gctttccagc cggggccaaa
      721 aatccccacc ggtgcaggcg tcttcgtccg gtagctgctg taggtgcggg agagaatgaa
      781 cggcagcggg ccgggcagcg caaggtccgt ctcgccgggc agcaccttcg cccccagcag
      841 cggatttacc gggttgcccg aagtcatccc gcccggacac accgagca
//
LOCUS       DAMZII010000340          888 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMZII010000340 DAMZII010000000
VERSION     DAMZII010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.347"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..533)
                     /locus_tag="POA77_004988"
     CDS             complement(<1..533)
                     /locus_tag="POA77_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HDI6368281.1"
                     /translation="MSTDITFDIRDEYKRKAIAEKIISLLESNIPVSPMVIDGDWGTG
                     KTEFSKKLASLIEINSTNHKVVYIDAFTEDHNDAPILTIMAAIAALYNSDEKKELISK
                     ALPALRFGLKTVLKAGAGWVLKQNADEINEEFEEVIKDAANSAIDGTIEALLEDHIEA
                     QKNVLALKEVLTKLAGE"
     gene            complement(547..729)
                     /locus_tag="POA77_004989"
     CDS             complement(547..729)
                     /locus_tag="POA77_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368282.1"
                     /translation="MLGKFFFVVLSCSLLLNPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKTCIK"
     gene            complement(745..>888)
                     /locus_tag="POA77_004990"
     CDS             complement(745..>888)
                     /locus_tag="POA77_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDI6368283.1"
                     /translation="GEITIRDITRLPGGRIFVEGGNRAFECKIEDVEIIGKIISLTVK
                     YVR"
BASE COUNT          245 a          181 c          141 g          321 t
ORIGIN      
        1 ctttctccag ctaatttggt tagtacttct tttagagcaa gaacgttctt ttgtgcctcg
       61 atatgatctt ctaacagagc ctcgatggtt ccatcgatag ctgaatttgc agcgtctttg
      121 ataacctctt caaattcctc attaatttca tccgcatttt gttttaatac ccaaccagca
      181 ccagctttga gtacagtctt aagtccgaat ctgagggctg gcaatgcctt agatattaat
      241 tccttttttt catcgctatt atatagagct gcaattgcgg ccattattgt aagtatggga
      301 gcatcgttgt gatcttcagt aaaagcatct atataaacta ctttatgatt ggtcgagttt
      361 atttcaatta aggatgctaa cttttttgaa aactctgttt ttccagtccc ccagtcccca
      421 tctatgacca taggtgaaac aggaatatta gactctagca aacttattat tttttctgca
      481 atggcttttc ttttatattc atcacgtata tcgaaagtta tatctgtgct cattcatatt
      541 tccttattat ttgatgcatg tttttttgga tttactgatg gttccatcat tgcaaacaaa
      601 ttttccatca gaggtacagt gagagacacc tccctttttc ccagaacaag gataattttt
      661 agcataggta gttagtgggt ttaataacaa agagcatgat aaaaccacaa aaaaaaactt
      721 accaagcata gtttcctcca ggtattatct aacatactta actgttaagc ttataatttt
      781 tccaattatt tcaacatctt ctatcttgca ctcgaaggct ctgtttccac cttcaacgaa
      841 aattcttcca ccgggtaaac gagtgatgtc gcggattgtt atttcgcc
//
LOCUS       DAMZII010000341          830 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMZII010000341 DAMZII010000000
VERSION     DAMZII010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.348"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>830
                     /locus_tag="POA77_004991"
     CDS             <1..>830
                     /locus_tag="POA77_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6368284.1"
                     /translation="LSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPEN
                     YNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYD
                     QILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKR
                     PSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLS
                     LDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAILSGSIYPPMKN
                     "
BASE COUNT          319 a          147 c          136 g          228 t
ORIGIN      
        1 ttatcggcta ttcatcacca gatcattgaa aaccaactgg atacagaaac aataactatt
       61 agttatgaat ttatcctctg tcaacatctt atcgcaaata ccgagttcag ctatttggca
      121 ctccctgaaa attataaccg tttgttttta ccaaattcaa aaaaccaaac aaataatcgt
      181 ttcaaaacgt taaacagcaa agctattggc aggctacttg ctgctggtgg cgtatacaat
      241 gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc aataaaaggc
      301 tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc atctattctt
      361 ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt aggcaaactt
      421 agaggtcaac aaaaacttct tgatggtata gacataatag aaataatata cattaagaga
      481 ccttcaaaag acttagctaa cttacgaaag gagtttaata aaactgtaag aaaaaatttt
      541 cttatcaaac ttgcaaaaac ctccgaagca tctggaagat tcaacgccga agacctttta
      601 agaatgagaa agggcaatgt tcctctaaat tataatgttc accataaact atctctagat
      661 gatggtggta ctaatgattt cgaaaattta gtattaatcg aaaacgaacc atatcataaa
      721 gtttttacta acatgcaatc acgaatagct aagggaatat tagtaggtga aagcaaaatc
      781 actccctggg ccattctatc tggctcaatt tatcctccca tgaaaaatat
//
LOCUS       DAMZII010000342          823 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMZII010000342 DAMZII010000000
VERSION     DAMZII010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.349"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            220..822
                     /gene="fimB"
                     /locus_tag="POA77_004992"
     CDS             220..822
                     /gene="fimB"
                     /locus_tag="POA77_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDI6368285.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          239 a          158 c          182 g          244 t
ORIGIN      
        1 atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaacgt tattataatt
       61 aactgttgct actccattta agttcactca gaagaactgg tccacttacg ttagttatta
      121 agcaaacgtt cgcttttata aacataatca ggataaaaat gttggattat tgctaaccca
      181 gcacagctag tgcgcgtctg taattataag ggaaaaacga tgaagaataa ggctgataac
      241 aaaaaaagga acttcctgac ccatagtgaa atcgaatcac tccttaaagc agcaaatacc
      301 gggcctcatg cagcacgtaa ttattgtctg actttgcttt gttttattca tggtttccgg
      361 gcgagtgaaa tttgtcgatt gaggatttcg gatattgatc ttaaggcaaa gtgtatatat
      421 atccatcgat taaaaaaagg cttttcaaca acgcatccgc tattgaacaa agaagttcag
      481 gctttaaaaa actggttgag tatccgtact tcgtacccgc atgctgagag cgagtgggta
      541 tttttatctc gtaagggaaa tccgctttct cggcaacagt tttaccatat tatctcgact
      601 tccggtggta atgccgggtt gtcactggag attcatccgc acatgttacg ccattcgtgt
      661 ggttttgctt tggcgaatat gggaatagat acgcgactta tccaggatta tcttgggcat
      721 cgcaatattc gtcatactgt ctggtatacc gccagcaatg cagggcgttt ttacggcatc
      781 tgggatagag ccagaggacg acagcgtcac gctgttttat aga
//
LOCUS       DAMZII010000343          823 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMZII010000343 DAMZII010000000
VERSION     DAMZII010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.350"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..605
                     /gene="rfaY"
                     /locus_tag="POA77_004993"
     CDS             <1..605
                     /gene="rfaY"
                     /locus_tag="POA77_004993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDI6368286.1"
                     /translation="LKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKK
                     DYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEIS
                     EDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDL
                     ERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK"
     gene            630..>823
                     /locus_tag="POA77_004994"
     CDS             630..>823
                     /locus_tag="POA77_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDI6368287.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIA"
BASE COUNT          304 a          116 c          147 g          256 t
ORIGIN      
        1 cattaaaagt ttttcgttct attgatgata ctaaagtcat tcttattgac actgctcgtg
       61 gacctcttgt acttaaagtt tatgcgccaa agcataaaat gaccgaacgt ttcttaaaat
      121 cttgcataaa aaaagattat tatgaaaatt taatttatca aaccgatcgg gtacgtggtg
      181 aaggcatcca atcaattaat gactattttt tgctagctga acgtaaaaca ttaaatttcg
      241 ctcattatta tattatgctc attgagtata ttgaaggtgt cgggttaaat gagtatttgg
      301 aaatttctga agatctgaag gatcaattat cagaatcgat aaaagagttg catcagcatg
      361 gaatggtgtc tggcgatcct cataaaggaa attttatagt atccgaaaaa ggtttaagac
      421 taattgacct ttcaggtaaa aaaacaaccg ctgttttaaa agcaaaagat cgcattgatc
      481 ttgaacggca ctataatata aaaaatgaat tgaaagactt tggctataca tatcttatat
      541 tcaaaaagaa aattaaaaaa gtaatcaggg atgtaaaagt caaactagga ttgaagagca
      601 aataatgatt atgatgaagg gtaattaaat tggattttaa acatcttact caatttaaag
      661 atataattga actggacaag cgccccgtta aacttgatga acgggaaacg tttaatgtct
      721 catggggtat tgatgagaac taccaggttg gggctgcgat ttcaattgct tcaattcttg
      781 aaaataataa acaaaacaaa tttacctttc acataatcgc tga
//
LOCUS       DAMZII010000344          806 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMZII010000344 DAMZII010000000
VERSION     DAMZII010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.351"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            16..>806
                     /locus_tag="POA77_004995"
     CDS             16..>806
                     /locus_tag="POA77_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6368288.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKE"
BASE COUNT          252 a          178 c          146 g          230 t
ORIGIN      
        1 taaccggagt agtgtatggc caatctctac gacttgaaaa agttcgactt aaaccttctt
       61 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
      121 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
      181 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac
      241 catcttgaga aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa
      301 ctaaagaaaa actttattat ctacggccct cagctcattt cttgctctaa taatagtatg
      361 ctgatccgct gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg
      421 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
      481 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta
      541 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
      601 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      661 gacgagagat ttatgaatcg caaaatctca tttcgtggtt cctctttgct gacaatcatt
      721 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taattcctac
      781 cgtgactttc ttaatttaaa agagat
//
LOCUS       DAMZII010000345          786 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMZII010000345 DAMZII010000000
VERSION     DAMZII010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.352"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>786
                     /locus_tag="POA77_004996"
     CDS             <1..>786
                     /locus_tag="POA77_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6368289.1"
                     /translation="GTQKHSFLLYAGAGSGKTRTLVNVLEMILKSHSEEFRLRGKKIA
                     VITYTNAACDEIKRRVKFNKLIDVSTIHSFLWELIRHHSSDIKSWLMENIAAEIADLR
                     DKQSRTTRVSQASLERERKIVSKEKRLQNLSDVYKFTYNPTGDNRTKDSLNHSEVIKL
                     GSYFLDRKYTMQKILVNRHPILLIDESQDTNKLLMNALLEVEKNNRDTFSVGLLGDMM
                     QRIYSDGKSDLEACLDEHWKKPEKSINYRSPKRVVALINLIRS"
BASE COUNT          299 a          125 c          151 g          211 t
ORIGIN      
        1 cgggcactca aaagcatagt ttccttcttt atgctggagc tggctctggt aaaaccagga
       61 ctttagttaa tgttctggaa atgattttga agagtcactc agaagagttc aggctaagag
      121 gaaaaaaaat agccgtgata acatacacca atgcggcttg tgatgaaata aaaagaagag
      181 ttaaattcaa caaattaata gatgtgtcta caattcatag tttcctatgg gagctaatta
      241 ggcatcactc cagtgatata aaatcttggt taatggaaaa cattgctgcg gagatagctg
      301 atttaaggga caaacaaagc agaacaacta gagtttcaca agcttcttta gaaagagaaa
      361 gaaaaatagt atccaaagaa aaacgtctgc aaaacttatc agatgtatat aaattcacat
      421 acaaccctac cggggataat agaactaaag actctctaaa tcattcagaa gtcattaaac
      481 tcggttctta ttttctagac agaaaatata caatgcagaa aatattggtt aatcgccatc
      541 caattctttt aattgatgaa agccaagata ctaacaaact gctaatgaat gcattactcg
      601 aagttgagaa aaataacaga gatacattct cggtgggtct gttaggggac atgatgcaaa
      661 ggatatactc tgatggtaaa agtgatttag aggcatgcct tgacgaacac tggaaaaaac
      721 ctgaaaaaag cattaactat cgttctccaa aaagagtggt tgctttaatt aatttaatta
      781 gaagtc
//
LOCUS       DAMZII010000346          751 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMZII010000346 DAMZII010000000
VERSION     DAMZII010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.353"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            17..448
                     /locus_tag="POA77_004997"
     CDS             17..448
                     /locus_tag="POA77_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HDI6368290.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTV"
     gene            519..>751
                     /locus_tag="POA77_004998"
     CDS             519..>751
                     /locus_tag="POA77_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDI6368291.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPA"
BASE COUNT          266 a          138 c          128 g          219 t
ORIGIN      
        1 gagtacaggt gggcatatgc aagtcttttc cagtgatgtc tacttcactg taggcactaa
       61 tgcattatta gcatcccaaa aagaatatta cagcgattta gttgcgttgg ttgatcttgg
      121 acattcgttc gttgttattg atgagcatca acatcgaaat ctcaaaccaa atacagaacc
      181 tgtaaacatc cttttgagca acaatttcat caggatcaat aagaatatta cattatctga
      241 tttaacgcat ttcctggttt caaatcttca tacacaaaat gtgtattcca cacaagaacc
      301 acttacccat gatgagatag atatcctgcg gttatgtgtt tcttactcgt taaaacaaat
      361 agccattatt aaaggaattg attataaaac aatatcatat cacaaaataa gagccctaaa
      421 taaacttaac ataaaaggta ctgtttagtt gtttatagca ctctgtgaat gggataaaca
      481 ttattttaaa ctccaatctt gcgtaagaga aagttgatat gtcattagta ggatataata
      541 gtaacatcat aataaccatc gcgctgatat cgttgctctc tgcatgcacc agagaaaatt
      601 cattaaagga gaatcaaaat aaaagaagtg gcgaatccca agcgatagta gatgcaagac
      661 agttctttag cgaacacccg gaatatatat catcagcaga actggagcaa aagctatatc
      721 gcgaatttgc acaggttact acagttccag c
//
LOCUS       DAMZII010000347          747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMZII010000347 DAMZII010000000
VERSION     DAMZII010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.354"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            14..616
                     /locus_tag="POA77_004999"
     CDS             14..616
                     /locus_tag="POA77_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HDI6368292.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
BASE COUNT          226 a          181 c          181 g          159 t
ORIGIN      
        1 agccgaggta tttatgggaa aaaatcagga agaaaggaaa actcccgtca ttgtggtgaa
       61 aaaacgtcgt actttttcgc taccgtcctt atccgaaaaa acagatatta tcgcacctgt
      121 atttactgaa cagacggcag aatcagcccc cgccggaata aattcatctg ccgtcgaaac
      181 acatatcccc gaagcgccag ccaggaagaa gaaaaagaaa agacaccgtt ttccacgtcc
      241 ctcccactgg acccgtgagt atactcacga gtgcgtggaa aaaataaagg ccctgtttcc
      301 tcatcttcgt gctgaaggtg gtggatttat tcccctgaaa attggcataa acaatgatat
      361 ttcggcattt ctggcagaac acccggagac agaactgacc atggacgaat ggctctgtgc
      421 agtgtcctgc atcacatccc gccgcgtata tctgcagcgg acagctgtcg ccggcgtccc
      481 acgttatggc ctggatggtc accccaaagg gcaggtctca gactctgaag cacagagtgc
      541 cggtcgcaga ctggcgacat tagaacaaaa gtggctcagg atgcaggcac agcaggagaa
      601 tatttctggg caataaacac tcctgataac aggtggacat tacgtatcag aaaaaatggg
      661 aagagaatgt tatgtgtgat tcagaagcgg agaagatttt attcatcctg aataaacatg
      721 aacgggcata cagacgcctg atccccg
//
LOCUS       DAMZII010000348          745 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMZII010000348 DAMZII010000000
VERSION     DAMZII010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.355"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            55..>745
                     /locus_tag="POA77_005000"
     CDS             55..>745
                     /locus_tag="POA77_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6368293.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          247 a          130 c          165 g          203 t
ORIGIN      
        1 cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga
       61 cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt
      121 tatgcaaaat tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa
      181 ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac
      241 gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga
      301 cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat
      361 gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga
      421 gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggatca
      481 gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact
      541 gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg
      601 gctctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa
      661 tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt
      721 gaagatgacg acgatgtaca aaaag
//
LOCUS       DAMZII010000349          732 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMZII010000349 DAMZII010000000
VERSION     DAMZII010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.356"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            62..490
                     /gene="tnpA"
                     /locus_tag="POA77_005001"
                     /pseudo
     CDS             62..490
                     /gene="tnpA"
                     /locus_tag="POA77_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          202 a          173 c          160 g          197 t
ORIGIN      
        1 ctcctgttga ccaccgggtc ttaactggaa tttaaatgct tgtagtcgtt tcacaggcca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac cagataccga cgtcagattt ttgaccatga
      181 cgcgacagaa aaactacgca cttacttttc aaatgtatgt gcttattttg aagctgaact
      241 ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc ctcccaaact
      301 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggtgac tgcgacgaga
      361 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtg ctggctattt
      421 tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
      481 aacacctggt caggtggaaa accgcgcctt atatccctgc cctgaaggac ggggttttac
      541 ggcgcaccgg ataagcctgg agattatcta caccagcatg gacatcattg tactatgccc
      601 cgtacatgcc ccatcacatc ctcaacatca aagtattaga gccttgcaaa ccattatgcg
      661 tctcagtttg tctcatctat cttacgcaaa aaaaaataga actaatatca agcggcgcta
      721 tattgcgtat ta
//
LOCUS       DAMZII010000350          725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMZII010000350 DAMZII010000000
VERSION     DAMZII010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.357"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..324)
                     /locus_tag="POA77_005002"
     CDS             complement(<1..324)
                     /locus_tag="POA77_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368294.1"
                     /translation="MEGKLIFCSDAILRFQSDYDETAAVPLLSIQNVIADTDPFFLLR
                     FFHHTVLIEEGTTLASIFLAIEPWKALLAAYLDRDVGAYIDEVRKPSGPTTWDIEWIG
                     IDRRSM"
     gene            complement(380..715)
                     /locus_tag="POA77_005003"
     CDS             complement(380..715)
                     /locus_tag="POA77_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368295.1"
                     /translation="MDKHYIPVLEDLRKAIYSDRMLSRLADSGNILIHSSLGYPVAKY
                     KNTGISIGIEPLNPMIRQDLTLGYIVIVRNGKASQEINGLLNRSLPKAISTFKEHINE
                     YESVKIKMR"
BASE COUNT          196 a          157 c          133 g          239 t
ORIGIN      
        1 catgctacga cgatcaatcc caatccattc aatatcccat gttgttggcc cggaaggttt
       61 tcttacctca tctatataag caccaacatc tctgtccaga taagccgcta acaaagcctt
      121 ccacggttct attgccagaa agatacttgc cagcgttgtt ccttcttcaa tcagaacggt
      181 atggtggaag aaacggagaa gaaagaacgg atcggtatcg gctatgacgt tctggatgga
      241 tagcaatgga acggcagcgg tttcatcgta atcggactga aaccgtagta ttgcatcgga
      301 acaaaaaatt aacttacctt ccatcgctca tcctcctcct gtagttatca gacaaaatat
      361 tttaccagat cttagattgt tatcgcattt tgatctttac tgattcatat tcgttgatgt
      421 gctccttaaa agtgcttatt gcttttggca atgacctgtt aagtaaacca ttaatctcct
      481 gtgatgcttt tccgtttctg actatcacga tatatccaag agttagatct tgtcttatca
      541 tcggatttaa tggttctatg cctatgctaa tccccgtgtt tttatattta gcaaccggat
      601 atccaagtga actatggata agaatattgc cgctatctgc aagccttgat aacattctgt
      661 cgctgtatat tgcttttctt aaatcctcta aaacagggat atagtgttta tccatgtttc
      721 gtgtt
//
LOCUS       DAMZII010000351          708 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMZII010000351 DAMZII010000000
VERSION     DAMZII010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.358"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            167..>708
                     /locus_tag="POA77_005004"
     CDS             167..>708
                     /locus_tag="POA77_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6368296.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAG"
BASE COUNT          201 a          146 c          153 g          208 t
ORIGIN      
        1 aagcccttaa agtgcacaat aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa
       61 atatcattgc tcattaatat ctagcatctg cttataacat ttgtgtcatg aatagtcatt
      121 cttcaaacca gtagtctgac taatggaatt ttattcaagg atttttatgt ctaaaaaatt
      181 aggttttgcc ctgtctggtc ttatgctggc aatggttgca ggtactgctt ctgctgatat
      241 ggatggtggt cagttaaata tcagtggtct ggttgttgat aacacctgtg aaacccgcgt
      301 tgacggcggt aataaagatg gtctgatcct gctgcaaact gcaactgttg gtgaaatcga
      361 tgctggcgtg ctgaatgaca ccgttggcgc taaagccaaa cctttcagca tcaccgttga
      421 ctgcagcaaa gcaaatccga atccaggtag tacagctaaa atgaccttcg gttctgtttt
      481 ctttggtaat agcaaaggaa cgctgaacaa cgacatgtct attaacaacc cgtctgacgg
      541 cgtgaatatc gctctgcaca atattgatgg ttccactatc aaacaggttc aaattaacaa
      601 ccctggcgat gtgtatacta aagccctgga tgcaacgaca aaatctgctg tttatgattt
      661 taaagcgtct tacgttcgtg ccgtcgcaga ccaaacagca actgctgg
//
LOCUS       DAMZII010000352          707 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMZII010000352 DAMZII010000000
VERSION     DAMZII010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.359"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..707)
                     /locus_tag="POA77_005005"
                     /pseudo
     CDS             complement(1..707)
                     /locus_tag="POA77_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT          217 a          149 c          112 g          229 t
ORIGIN      
        1 tttgggtcaa acgacttagc aaagtcaatt aatttcggtg cagcatctcg cattttgctt
       61 aaaatatcag gttcgagctt tccttcattc accagaagtg acattttatc cagatagtca
      121 tcaaatttca tcccccccgg tccgccagat gcttcatctc tcccaggtac ttcttcatat
      181 cacgttgaga taattcttca aactgagctg tcagatagtt ctcattatat tcattaatat
      241 tcactttttc tgctccggct aaatctgtgt aggcactaga atatgaaatt gaaattaaca
      301 gcgaagcaaa taattttaag ctgttgtcat catgttcctg gcaggcatta acaaacgtta
      361 aaaaaccaga acctattgct tgaaaatgta tattcgctaa aggttcatta tccttagatt
      421 cctcataaaa tagctgaacc gtggagggaa ggccattctt agtttctaca tcaaaagtac
      481 atcgttcaat gggagtcgca tctttattat ccccaggatt acatatacta ttaatcgcga
      541 aatgaaaaat agcccggtca acaactgatg caataaagtc acgatcgttt aaatcctcat
      601 cagtgcaatc gtcaatatac ttattgaccc acattttgag atcatttatt tgctcaatta
      661 aaaacacatt ttttacgtgc atgccataaa ttatgcgtga ttcgttg
//
LOCUS       DAMZII010000353          686 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMZII010000353 DAMZII010000000
VERSION     DAMZII010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.360"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(102..554)
                     /locus_tag="POA77_005006"
     CDS             complement(102..554)
                     /locus_tag="POA77_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368297.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGFRNVQNGTTRNVVKDQQLLKQLRKVDSGK"
BASE COUNT          169 a          207 c           98 g          212 t
ORIGIN      
        1 ggttttactt ttacattaaa tactcgacca ctctgacttt caaaatagtg aatggacact
       61 tttcgacctg acgcatcaaa tccatcttta taaactttac tctattttcc tgaatctact
      121 ttacgtaatt gtttcaataa ctgctgatcc tttacaacat tccttgtagt gccattttgg
      181 acattcctaa accctctggg atactgagga gcatccttaa tagacctgta ggtatttctt
      241 atttcgccat cctcatctgg ctcccaccct cccgaacctc ctgtactgcc actgcccaaa
      301 cagttcttct cttcatccgt caaatccttc ccgacatttg gattctcttt tgatgaatca
      361 tcatttccaa acacgctgga aaaagcacca tagggatctc gccagaattc catttgactt
      421 tgtgcattta ataattcggc ttcaccccgc atctccatac ttttctcaat cgcatcccca
      481 acagcctcgc gattgttgta tattactata ccaccaccaa taaccaccgc tccaactgcc
      541 gcaccgacta acatccctgg agggcctcct acccagggca agattacgaa agcccgctcc
      601 ccgcaaggac tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta
      661 accctgcctt gaatacctta tcattc
//
LOCUS       DAMZII010000354          668 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMZII010000354 DAMZII010000000
VERSION     DAMZII010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.361"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..476)
                     /locus_tag="POA77_005007"
     CDS             complement(<1..476)
                     /locus_tag="POA77_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDI6368298.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLL"
     gene            complement(485..652)
                     /locus_tag="POA77_005008"
     CDS             complement(485..652)
                     /locus_tag="POA77_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein"
                     /protein_id="HDI6368299.1"
                     /translation="MLARYASQLNAFAISLTVKSALAFLILIIYFAPILAERVMPLSF
                     FPEQLQLYIEK"
BASE COUNT          229 a          121 c          112 g          206 t
ORIGIN      
        1 agcagtgatt tatgattact ccaaaatact ttgatcgcca atgcaaaaaa aacaatataa
       61 agaattgctt taacaaattc ttttacggtt tttaagccaa atatacgctt taagccattt
      121 actggattca atgaatcaaa cttaaattta acagcttcag ttgctaattg cattttactt
      181 tgaaaccagg agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgttcgtt
      241 ttcaaccaac caacccccat agctttaaaa tagtcccaaa tatctggttt agcgttatga
      301 tcaaggatgt attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat
      361 aaagtaccca caagcattat tacagagatg gttaagtctc tactttttag aatttgccct
      421 tttttagaag catcctggag tttcttttgt gttggctttt cagttttatt ggccatgaaa
      481 cactctattt ctcaatgtat agttgcaatt gttctgggaa aaaggataac ggcattactc
      541 gttcagccag tattggggca aaataaataa ttaatattaa aaatgccagc gcacttttga
      601 cagtcagtga aatggcaaaa gcatttagtt gtgaagcata acgtgccagc aatcccagga
      661 cagcctca
//
LOCUS       DAMZII010000355          662 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMZII010000355 DAMZII010000000
VERSION     DAMZII010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.362"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            89..>662
                     /gene="traJ"
                     /locus_tag="POA77_005009"
     CDS             89..>662
                     /gene="traJ"
                     /locus_tag="POA77_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HDI6368300.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHAS"
BASE COUNT          214 a          118 c          123 g          207 t
ORIGIN      
        1 ttaaaatttg aatttgaaaa tcgccgatgc agggagttct ctcctccctg catcgactgt
       61 ccatagaatc ctttgtgagg aggttcctat gtatccgatg gatcgtattc aacaaaaaca
      121 tgctcgtcaa atagatctgc tggaaaatct gacggcagtt attcaggatt atccaaatcc
      181 agcctgtatc agggacgaaa ctggaaaatt tattttttgc aatacgctgt ttcatgagtc
      241 atttcttaca caagatcaaa gtgctgaaaa atggcttctg tcgcagagag atttttgtga
      301 attgatctct gtcacagaga tggaagcata caggaatgag catacgcatc ttaatcttgt
      361 agaggatgtt tttattcaga acagattctg gacaatatct gtccagtcat ttcttaatgg
      421 acacagaaat attattctgt ggcaatttta tgatgctgct catgttcgtc ataaagacag
      481 ttataatcaa aaaacgattg tcagtgatga tatcagaaat ataatcagaa gaatgagtga
      541 tgattcttct gtatcatcat atgtaaatga tgtcttttac ttatatagca ccggaatcag
      601 tcataatgct atagcaagaa tattaaatat atccatctcc acatcaaaga aacacgcatc
      661 tc
//
LOCUS       DAMZII010000356          657 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMZII010000356 DAMZII010000000
VERSION     DAMZII010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.363"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            157..381
                     /locus_tag="POA77_005010"
     CDS             157..381
                     /locus_tag="POA77_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368301.1"
                     /translation="MELAKDSPDAKQELLERLLSGKRMTGRDEMDIKRILKTKEALDN
                     IRNFIDKQQLTNSDTPKKSTVPNEDKKGIR"
     gene            381..647
                     /locus_tag="POA77_005011"
     CDS             381..647
                     /locus_tag="POA77_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368302.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
BASE COUNT          226 a          115 c          139 g          177 t
ORIGIN      
        1 attctcctga ttgtatacct gtatttctgt atagttgaca atgagtttct cagaatcggt
       61 ttctgtattg catggacagc atactacttt ctttcgcttt tcaagtattt tgtaaaatac
      121 ttctttcctc tcgatcgtga agatttgctt aaactgatgg agctcgccaa agattctcct
      181 gatgcgaaac aggagcttct tgagcgtctt ttgtccggaa aaagaatgac cgggcgggat
      241 gaaatggata tcaagcgcat tctaaaaaca aaggaagcac ttgataacat caggaatttt
      301 attgataaac agcagttaac gaatagtgat actccgaaga aaagcacggt cccaaatgaa
      361 gataaaaaag gcataagatg atgatcacag gaaatggaat aaacactgta actgtcaatg
      421 gaaaagtgaa acacatcaca gaactggatg atataacact ttgtttagag tggacaaaac
      481 tacgagagga aaacaatcgt ctttatgaaa ttaataacca ggcaaacaga ggatggcgtg
      541 gtttcatcct aagattgata ggggttaacc tgccagataa aaggactgag tttacacaac
      601 gtctcttatt gacgcgcaaa atttcgggtt cggtcatgaa aaagtaacaa aaccgcg
//
LOCUS       DAMZII010000357          646 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMZII010000357 DAMZII010000000
VERSION     DAMZII010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.364"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>646
                     /locus_tag="POA77_005012"
     CDS             <1..>646
                     /locus_tag="POA77_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368303.1"
                     /translation="NMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSYW
                     EDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIA
                     EYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLN
                     DSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPD"
BASE COUNT          231 a           92 c          130 g          193 t
ORIGIN      
        1 taaatatgat tgtaatatat actagagaga atttagaaac ggtttggatg cagatatcat
       61 cgactctcaa aggtgctctg gatatcaaca gcttgatcat tgactacgat aatgaagatg
      121 tccaaagtta ttgggaagac gttgtattac cgaacttaaa tgataatggt aataaagctc
      181 tcacaagaga tgaaacaata gcctatatta aagacagtaa gccttgtaga agaattaaaa
      241 gattaataca tgatgatgct gtgttggagg atcaaaagga taaaaacttc attgcaaaaa
      301 tgattgcaga atatgctgtg tctagaaatg caattatttc tagcaacaca tctggcaatg
      361 tcattcgggg tgatgaaagc ggagtaaaat ggattcaatg tggtaatatc tttgtctccc
      421 tatttcataa ggttcaagat gatcatgaaa acgatggaga taggatttgg caaactctca
      481 atgattctct cattgaatgg aaaccatctt attatcagtt aataaaatct gaaattcaga
      541 atgcaatcga agctgaggct ttatcttttg taaatcattt ggctaacgat cattacggtc
      601 aagctgcgtg gttaaatgag atattaaaat cagactcgcc tgatat
//
LOCUS       DAMZII010000358          643 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMZII010000358 DAMZII010000000
VERSION     DAMZII010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.365"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(162..554)
                     /locus_tag="POA77_005013"
     CDS             complement(162..554)
                     /locus_tag="POA77_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368304.1"
                     /translation="MKYILTVLCIFFCQTASAYPYRIYTAPEGAIVKNILTNEVIGKT
                     PVEVDVSNTEAGSTFGISMYRHENVAIKIFTVMPNAENNFTVSGPDVATMSLPGKAPL
                     NVTNDSNAAAVHIDLRPFLSEPAHHPVQ"
BASE COUNT          206 a          112 c          125 g          200 t
ORIGIN      
        1 ttagatgcgg atgaaattat tcatgtacgc ttttatttag tctacgcatt accaagtcct
       61 aaaagaaatc aatagcaaaa atatcttaac tatctgataa tgatgggttt taacatatac
      121 ggtattgtta agcaggcccc aaagattggg gcctagaagt gttattgaac agggtgatgg
      181 gccggttcag acagaaaagg tcttagatct atatgaaccg ctgctgcatt actgtcattg
      241 gtcacgttta acggggcctt acccggtaaa gacatagtgg caacgtctgg accagaaaca
      301 gtaaaattat tttctgcatt tggcattact gtgaaaattt taatagctac attctcatga
      361 cgatacattg aaattccgaa agttgatcca gcttcagtat tgctgacatc aacttcaacc
      421 ggtgttttcc cgatcacttc attagtaagt atatttttca caattgcacc ttctggggca
      481 gtgtaaatgc gataagggta agcacttgca gtctggcaga aaaaaataca caaaacggtc
      541 aatatatatt tcatgattgc tccttttgca aaaattctaa atcaaatgat tgagtgacta
      601 caatttgttt ggaatcaaat gaacctggga atttatattt aat
//
LOCUS       DAMZII010000359          635 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMZII010000359 DAMZII010000000
VERSION     DAMZII010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.366"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..119)
                     /locus_tag="POA77_005014"
     CDS             complement(<1..119)
                     /locus_tag="POA77_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368305.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIY"
     gene            complement(214..>635)
                     /locus_tag="POA77_005015"
     CDS             complement(214..>635)
                     /locus_tag="POA77_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368306.1"
                     /translation="KESFIESIIQIEFNSNSTNCLEKLCNEVSILFKNQPDYLTFLRA
                     MDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRNNDDFINPDLQERLVIGDDSISIFTY
                     DIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          217 a          103 c           91 g          224 t
ORIGIN      
        1 aaataaatca acatgtcatt aaaatcatca tagccattag ctatttcggc taaaatagag
       61 actacatgtc ttcggtccat ctcacttaag gagtgtagtt ccgttgtaag tttttccata
      121 gcttgcactg ctaaatttcg aacaaggaat tttctgctgg taatctctaa aaagatggca
      181 tggtttacaa tgatttttgt ttccttttga ttattatgaa caactgtcca tgatttcgtt
      241 taagaatgaa gagaaatcac taaacgaact gaatatattt tctgtgccaa tattatctct
      301 aatttcaaaa aagttacttt taatgtcata agtaaatatt gaaatgctat catccccgat
      361 cactaaccgt tcttgtagat cagggtttat gaaatcatca ttatttctat aaaattcatt
      421 tacggcaaaa aggtttttaa ctgaaggttc tggaatcgag aggctaaata atcgtaatcc
      481 attaacttcg aatccatcca ttgctcttaa aaaagtaaga taatcaggtt gattcttaaa
      541 aagaatacta acttcattac ataacttctc caggcaatta gttgaattag aattaaattc
      601 tatttggatt atactttcaa taaatgattc cttga
//
LOCUS       DAMZII010000360          632 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMZII010000360 DAMZII010000000
VERSION     DAMZII010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.367"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..531)
                     /gene="fimE"
                     /locus_tag="POA77_005016"
     CDS             complement(<1..531)
                     /gene="fimE"
                     /locus_tag="POA77_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDI6368307.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAA"
BASE COUNT          190 a          163 c          130 g          149 t
ORIGIN      
        1 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
       61 ctgaattaaa cgagtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
      121 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
      181 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcagtccg
      241 gtcagcgcct ttccagttag cacgttcctg ggtccatcgt tccacggctt cacgctcatc
      301 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattctacc
      361 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
      421 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
      481 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
      541 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
      601 gcccaataga taaaaagata accacacacg ga
//
LOCUS       DAMZII010000361          631 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMZII010000361 DAMZII010000000
VERSION     DAMZII010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.368"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..543
                     /locus_tag="POA77_005017"
     CDS             <1..543
                     /locus_tag="POA77_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HDI6368308.1"
                     /translation="QELNRTETAREVQRPSIDAHLLWLEAELKRLEKQIKDLTDDDPD
                     MKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRYESGSSVRGA
                     SRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMMRKLAQVA
                     YGVLKSGVPFDASRHNPVAA"
BASE COUNT          162 a          147 c          206 g          116 t
ORIGIN      
        1 caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat tgatgctcac
       61 cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga cctgacagac
      121 gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg tatcggagag
      181 aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc ccatgccaga
      241 cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag cagtgtgaga
      301 ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt gtatatgccc
      361 gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg tctggcggct
      421 aatggcaaga aaggaaaggt gattctcggc gcgatgatgc gcaagctggc acaggtggcg
      481 tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac ggcataatcc ggtagcggcg
      541 taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg cgctgtgtct ggcatgcctg
      601 caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAMZII010000362          620 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMZII010000362 DAMZII010000000
VERSION     DAMZII010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.369"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            349..>620
                     /locus_tag="POA77_005018"
     CDS             349..>620
                     /locus_tag="POA77_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368309.1"
                     /translation="MHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAV
                     FLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYYV"
BASE COUNT          194 a          103 c          123 g          200 t
ORIGIN      
        1 gaatacgcta acagcggagt tgctgcattg ctccaatatt gctcttcaaa aatggatctg
       61 ccatcaaata atgatactta tcacgaaatg tatcatgctt taaatatgaa tttgtcttca
      121 aagaattttg aagatggtca tatttctact ggcactattt tctttgatac agagtcgaac
      181 aaatggtatt tatgtgtatc tgcggcatgt gatttggttc ctactcagtg taacgaccct
      241 caccatgtaa gattaagtcc gcacaggctc attaaagttc tggagctttt taacgccagt
      301 cagagtaaag cattgccatt tgctgaacat tcgaaatata tatatgtaat gcataaaaat
      361 caaagaaaat atctctctat tttcgaaggg gataaaacgc ttcctgttgt tgattatatg
      421 gtggtgttga atcatggaac aacagttgat ggcgaagaaa aaaatattat ttctgccgtg
      481 tttttaagta atatggatgg caacgtgcaa aatgttcctg tccgactcaa acttaaatct
      541 caactgagaa ctggttatgc agaaagatat caggctatag cgtctcagta tagctcaagg
      601 attggtgtgg attattatgt
//
LOCUS       DAMZII010000363          612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMZII010000363 DAMZII010000000
VERSION     DAMZII010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.370"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            6..>612
                     /locus_tag="POA77_005019"
     CDS             6..>612
                     /locus_tag="POA77_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDI6368310.1"
                     /translation="MHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQDPIGLEGG
                     WSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTD
                     QFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDN
                     KKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQDAG"
BASE COUNT          195 a           98 c          159 g          160 t
ORIGIN      
        1 atcacctgca ccagccgtac cggctgccgg ggcagcagta tgataaggag tcggggctgt
       61 actacaaccg gaaccggtac tacgatccgt tgcaggggcg gtatatcact caggacccga
      121 tagggctgga ggggggatgg agtctgtatg cgtatccgct gaatccggtg aatggtattg
      181 atccattagg gttaagtcct gcagatgtag cgttaatgcg aaaaaaagaa caactaaacc
      241 atcaaagagc atgggatata ttatctgata cttatgatga tatgaagaga ttaaatttag
      301 gtgggactga tcaatttttc cattgtatgg cattttgtcg agtgtctaaa ttaaatgacg
      361 ctggtgttag ccgatcagcg aaagggctgg gttatgaaaa agagattaga gattacgggt
      421 taaatatgtt cggtatgtac ggcagaaaag taaagttatc ccattctgag atgattgaag
      481 ataataaaaa agacttggct gtaaatgaac atgggttgac atgtccatta acacaagatt
      541 gctcaaatag atgtattgat tatattaatc cagagcataa aaaaacgata aaggctttac
      601 aagatgctgg ct
//
LOCUS       DAMZII010000364          605 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMZII010000364 DAMZII010000000
VERSION     DAMZII010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.371"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            94..>605
                     /locus_tag="POA77_005020"
     CDS             94..>605
                     /locus_tag="POA77_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6368311.1"
                     /translation="MKWQGRAILLPGIPLWLIMLGSIVFITAFLMFIIVGTYSRRVNV
                     SGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRNGIVTDNHRR
                     DIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKIMKNN
                     MENYRYYQSKG"
BASE COUNT          201 a          103 c          136 g          165 t
ORIGIN      
        1 tttattttcc atcattaatc cagataaaca acaaactaat agtatgcaag gagacattat
       61 ttgtttcgcc aggatgcttt agaaaacaga aaaatgaagt ggcagggacg ggcaatatta
      121 cttcccggaa taccactgtg gttaatcatg ctgggaagca ttgtgtttat tacggcattt
      181 ctgatgttca ttattgttgg tacctatagc cgccgtgtta atgtcagtgg tgaggtcaca
      241 acctggccaa gagctgtcaa tatatattca ggtgtacagg gatttgttgt caggcagttt
      301 gttcatgaag ggcagttgat aaaaaaaggg gatcctgttt atctgattga catcagtaaa
      361 agtacacgca atggtattgt cactgataat catcgccggg atatagaaaa ccagctggtt
      421 cgtgtggaca acattatttc ccgtctggaa gaaagtaaaa aaataacgct agataccctg
      481 gaaaaacaac gtctgcaata cacagatgcg ttccgtcgct catcagacat tatacagcgt
      541 gcagaggaag ggataaaaat aatgaaaaat aatatggaga attacagata ctatcagtca
      601 aaagg
//
LOCUS       DAMZII010000365          598 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMZII010000365 DAMZII010000000
VERSION     DAMZII010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.372"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>598
                     /locus_tag="POA77_005021"
     CDS             <1..>598
                     /locus_tag="POA77_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HDI6368312.1"
                     /translation="PLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKS
                     GACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYF
                     STAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGAGSHGEYWDEIFSGKDEKIVKSKDN
                     NIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGN"
BASE COUNT          206 a          101 c          136 g          155 t
ORIGIN      
        1 cccgctgcag gggcgatata tcactcagga tccgattgga ctgaaggggg gatggaacct
       61 gtatggatat caattgaatc cgatatcaga catcgacccc ctgggtttat ctatgtggga
      121 ggatgcaaaa tcgggggcat gtactaatgg tctttgcggc acactatccg ctatgatagg
      181 tccagataaa tttgattcta tagatagcac cgcatatgac gccttaaata aaataaatag
      241 ccaatctatt tgcgaagata aagagttcgc tggtttaata tgtaaggata atagtggcag
      301 atatttctca acagcaccta accgaggaga aagaaaagga tcatatccat tcaatagccc
      361 ttgccctaat ggtactgaga aagtatcagc ttatcatact catggtgcag gtagtcatgg
      421 agaatattgg gacgaaatat tttcaggtaa agatgagaaa atagttaaaa gtaaagataa
      481 caatatcaag tcattttatt taggtacgcc cagtggtaat tttaaagcaa tagataacca
      541 cgggaaggaa ataacaaaca gaaaaggatt acctaatgtc tgcagagttc atggtaat
//
LOCUS       DAMZII010000366          596 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMZII010000366 DAMZII010000000
VERSION     DAMZII010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.373"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..83
                     /locus_tag="POA77_005022"
     CDS             <1..83
                     /locus_tag="POA77_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDI6368313.1"
                     /translation="EYKEQEGNPEIKSKRRERIRKFFLSN"
     gene            80..472
                     /locus_tag="POA77_005023"
     CDS             80..472
                     /locus_tag="POA77_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDI6368314.1"
                     /translation="MKSDVSNSRLMISNPTHIAIGIYFKPHLSPIPLISVRVTNEVAL
                     GVRKYAKEIGIPIITDKKLARKNYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPV
                     QDEQLSSEDKFIEGEDTKIENKDDNLKN"
BASE COUNT          220 a           98 c          114 g          164 t
ORIGIN      
        1 gagaatacaa ggagcaagaa ggaaatccag aaattaagtc taaacgcaga gagcgcatca
       61 ggaaattctt tctgagcaat tgaagtctga tgtcagtaat agtcgtttga tgatttccaa
      121 ccctactcac attgcaatag ggatatactt taagccacat ctttcaccta ttccattgat
      181 ttctgtaaga gtaactaatg aggtggcctt aggcgtaagg aaatatgcaa aggaaatcgg
      241 aataccaatt atcacagata aaaaattagc acgaaaaaat tatgctaccc atcgtcgcta
      301 cgattatgtt agcttcgaaa atatagatga aatattacgt cttctgctgt ggcttgaaga
      361 tgtggagaat gctgggcaac ctgttcaaga tgaacagctc tcttcagaag ataaattcat
      421 tgagggtgaa gacacaaaaa tcgagaataa agacgataat ttaaaaaatt aaacatctgt
      481 cgtagtcaga aatttataag aatctacacg caaaaagcgt gtagatcctt atttaatgag
      541 ttaaacgatc ttgtgaaatg ataagcaatt gatacatact catctcttta taagct
//
LOCUS       DAMZII010000367          588 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMZII010000367 DAMZII010000000
VERSION     DAMZII010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.374"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(206..>588)
                     /locus_tag="POA77_005024"
     CDS             complement(206..>588)
                     /locus_tag="POA77_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI6368315.1"
                     /translation="EETINIEYIEGTNVRFNSNNMAIYWNSRASLFCSTELNSKSQSP
                     ALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRG
                     AHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          179 a          144 c           76 g          189 t
ORIGIN      
        1 ttcaccacca ctttatatct gtatatttca ttaagacctt ccttgctaag cggaactaac
       61 gtggtaacat acgatggaag attaattatc tctatcgttt tcacgctatc tattccacac
      121 aattcattca ctcgccccat ccccagtacc gaaccagaaa atagagacaa taaaaccatc
      181 aaatatttca tcataaaaaa cctcactatt ctggcgtacc cgttattttc atcgtctgca
      241 acggtccatc cacccgataa aaaggcagtc cactatgagc tcctctggta cactcaccta
      301 aagttttcgc cgcacgcgat tcaattattg taataaccct tgcctcttct ttattctcat
      361 atttcttatc tgttcggctt aaaagagcca tgaaattttc cttatcaaga aggtaatact
      421 gcgcatgagc aaactcatgt cccaaaccta atgcaggcga ttgtgatttc gagtttaatt
      481 cagttgaaca aaataaagat gctctactat tccaatatat cgccatatta ttcgaattaa
      541 atctaacatt ggttccttca atatactcta tatttatagt ttcttcag
//
LOCUS       DAMZII010000368          587 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMZII010000368 DAMZII010000000
VERSION     DAMZII010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.375"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..258)
                     /locus_tag="POA77_005025"
     CDS             complement(<1..258)
                     /locus_tag="POA77_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDI6368316.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKI"
     gene            complement(301..537)
                     /locus_tag="POA77_005026"
     CDS             complement(301..537)
                     /locus_tag="POA77_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDI6368317.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          162 a          128 c          104 g          193 t
ORIGIN      
        1 tattttctta ccattgaaca tacttttatt gtcagctgtc gctgttcttc ggtttggccc
       61 tcctgaatca ctattgctaa aatcacgaat tatgctgtca attaaatcgc gtcggaaagc
      121 atcttcctca gccgatttca ttacgagtaa ttgcttaata agagtctgca atgaaaaatt
      181 tatcttcatg cgcagtatct ggagcaccgc ctggccagta atttcatatt ttatacgttc
      241 attatcagat gtcttcataa cgacttcata gctaacctgt taaataatca ggctgttgca
      301 ttattcaaaa agcaacccaa ttagtgcatt ataatgtttc tgttcctctt cccctgtcgc
      361 ttgttccagt cggcaaagaa gtttggcaca aagcgacttt cgactcagat tacgtccatc
      421 tttcaaaatc tcagtcacaa tttccccgag agtttcctgc tgagtaggca agttagcttt
      481 tgcaaagtaa cgagtgattg ctcccgcaga atctgaagtc actgaatttt gatgcatgct
      541 acgcctctgt taaaaatgtt aatcaatgtg aagtgaggta acaatag
//
LOCUS       DAMZII010000369          581 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMZII010000369 DAMZII010000000
VERSION     DAMZII010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.376"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>581
                     /locus_tag="POA77_005027"
     rRNA            <1..>581
                     /locus_tag="POA77_005027"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAMZII010000370          578 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMZII010000370 DAMZII010000000
VERSION     DAMZII010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.377"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(316..>578)
                     /locus_tag="POA77_005028"
     CDS             complement(316..>578)
                     /locus_tag="POA77_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368318.1"
                     /translation="AERVRDQRLELIPKELTVNPKPETLEILTPIESAKKNDSGLNTS
                     HGSAAVSASQSENTVLSEHAQIQGVTRKPKKKANANLANLAK"
BASE COUNT          160 a          132 c           97 g          189 t
ORIGIN      
        1 tcgtgcaaac aaagaacagt aaacggggcc taaaccacct ttttttagtg gtttacctaa
       61 ccaaaattaa tttatccaaa atcgtaactg gttcgacctg aatcaaccca cgcgaatttt
      121 aatgatttct tagaactggc tctataattc ttcaaactgg cttaactgga gatgtgacaa
      181 cgagggattc atttagtaca tgaaaccggt ttaccattcc ccctcacctg ttaataatat
      241 accaaaaaaa agccagctgg taagctgact ttttattaat agataactca atctaatcaa
      301 ttgatattac tatacttact tcgcaagatt tgcgaggttt gcattggcct ttttcttcgg
      361 cttccttgtc acaccttgga tttgcgcatg ttcactcagc accgtgtttt cgctttgtga
      421 ggcgctaaca gcagcagaac catgactagt atttaaacct gaatcattct tttttgcgct
      481 ctcaatcggc gttaaaatct ctaaagtctc aggtttggga tttacagtca gttctttagg
      541 tattaattcc agccgctgat cccttaccct ctctgcct
//
LOCUS       DAMZII010000371          568 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMZII010000371 DAMZII010000000
VERSION     DAMZII010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.378"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            31..540
                     /locus_tag="POA77_005029"
     CDS             31..540
                     /locus_tag="POA77_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDI6368319.1"
                     /translation="MILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTVNLGEVA
                     DTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLKNTKETE
                     IGGATGVGVRILDSQSGEVTLGTPVIITFNNTNSYQELNFKARMESPSKDATPGNVYA
                     QADYKIAYE"
BASE COUNT          170 a          128 c          129 g          141 t
ORIGIN      
        1 aaaaagtcac ttcttgttgc tggcgtagca atgattcttt ccaataacgt atttgctgac
       61 gaaggtcacg gcattgttaa atttaaaggt gaagttatct ctgctccatg ttcaattaaa
      121 ccaggtgacg aagacttaac tgtaaatctg ggtgaagtag cagataccgt tctgaaaagc
      181 gaccagaaat ctttggctga gcctttcact attcacctgc aagactgcat gttgagccag
      241 ggtggaacca cttattcaaa agcgaaggtt actttcacca cagcgaatac tatgacgggt
      301 cagactgacc tgctgaaaaa caccaaagaa actgaaatcg gtggtgccac aggcgttggc
      361 gtacgtattc tggatagcca gagcggcgaa gtgactttag gtactccggt aataattaca
      421 ttcaacaata ctaacagcta tcaggaactg aacttcaaag ctcgtatgga atccccaagc
      481 aaagatgcta ccccgggcaa cgtttacgct caggctgact ataagattgc ttacgagtaa
      541 tcacattagg ccgggttcgc ccggccac
//
LOCUS       DAMZII010000372          550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMZII010000372 DAMZII010000000
VERSION     DAMZII010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.379"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>550
                     /locus_tag="POA77_005030"
     CDS             <1..>550
                     /locus_tag="POA77_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368320.1"
                     /translation="VALPGNCPKLLKNQILHDISFAAETDYLFTLEEYSAELGGLVFD
                     WAPEDVYQLYVVAMEESLENVRHLVITKSLYSTDEFGNITVNPLLEAETRWYMSKSFE
                     LTCATHGIDAIEFRSELKKSLYEYTHSYGGENAELARYHQDKEVILHDCKEEMGWDIF
                     FEQDYLLQENKLAMKWTDRDIMD"
BASE COUNT          167 a           93 c          128 g          162 t
ORIGIN      
        1 tgttgctttg cctggtaatt gtcccaaact actcaaaaat caaattctgc atgacattag
       61 ttttgctgcg gaaactgatt atctcttcac tctagaagag tacagtgcgg agttaggtgg
      121 gctggttttt gactgggccc ctgaagatgt atatcagtta tacgtcgtcg caatggaaga
      181 gtcgttggaa aacgtaaggc atctcgtcat tacaaaatca ctctattcca ctgatgaatt
      241 cggtaacata accgtgaatc cgcttcttga agccgaaaca cggtggtata tgtcgaaatc
      301 atttgagctt acatgtgcca ctcatggtat tgatgctatt gagttcaggt cagagcttaa
      361 aaaaagctta tatgaatata cgcacagtta cggaggtgaa aatgctgaat tagctcgtta
      421 tcatcaagat aaagaggtta ttctgcatga ttgtaaggaa gagatgggat gggatatctt
      481 tttcgaacag gactatttgt tgcaagaaaa caaactggca atgaaatgga ctgatcggga
      541 tataatggat
//
LOCUS       DAMZII010000373          540 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMZII010000373 DAMZII010000000
VERSION     DAMZII010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.380"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            183..>540
                     /locus_tag="POA77_005031"
     CDS             183..>540
                     /locus_tag="POA77_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6368321.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPA"
BASE COUNT          130 a          133 c          141 g          136 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
//
LOCUS       DAMZII010000374          535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMZII010000374 DAMZII010000000
VERSION     DAMZII010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.381"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..495
                     /locus_tag="POA77_005032"
     CDS             112..495
                     /locus_tag="POA77_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDI6368322.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
BASE COUNT          169 a           79 c          120 g          167 t
ORIGIN      
        1 ttttttatag gataccgcta ggggcgctgc tagcggtgcg tccctgtttg cattatgaat
       61 tttagtgttt cgaaattaac tttattttat gttcaaaaaa ggtaatctct aatggctaag
      121 gtgaacctgt atatcagcaa tgatgcctat gaaaaaataa atgcgattat tgagaagcgt
      181 cgacaggaag gggcaaggga aaaagatgtc agtttttcag caacagcttc aatgcttctt
      241 gaactggggc ttcgtgtaca tgaggctcag atggagcgta aagagtctgc atttaatcag
      301 actgagttta ataaattgct tcttgaatgc gttgtaaaaa cacaatcatc agtagcgaaa
      361 attttgggta ttgagtctct cagtcctcat gtctccggaa atccaaagtt tgaatatgcc
      421 aatatggttg aagatatcag ggagaaggta tcatctgaga tggaacgatt ttttccaaaa
      481 aatgatgatg aataaaagaa atttgacttc gttcaaatat cagagttttt atgat
//
LOCUS       DAMZII010000375          531 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMZII010000375 DAMZII010000000
VERSION     DAMZII010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.382"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..531
                     /locus_tag="POA77_005033"
                     /pseudo
     CDS             1..531
                     /locus_tag="POA77_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          175 a           77 c           99 g          180 t
ORIGIN      
        1 atgatactat aagtgtaaaa ttctacagaa atgaaaaaaa ttgaatgcgc ttgcaatttt
       61 ctgatggata aagatgcgca ggggtatatc gacctgtctg atttggattt aacaagttgt
      121 cattttaaag gtgacgttat atcgaaggtg tcttttttat catcaaatct acaacatgta
      181 acattcgaat gtaaagaaat tggggattgc aattttacta ctgcaatagt tgataatgtc
      241 atatttagat gtcgacgttt acacaatgtg atttttatca aagcgagtgg tgaatgtgtc
      301 gatttcagca aaaatattct tgatacagtt gacttctcgc agaatcaact tactcagagt
      361 aattttcgcg aatatcagat tagaaattca aacttcgata attgttatct ttacgcttcg
      421 cacttcacca gagcagagtt tctgtctgcc aaagaaatat catttattaa atcgaatatg
      481 acagctgtta tgtttgatca tgtgcgaata tcgacaggga attttaaaga t
//
LOCUS       DAMZII010000376          511 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMZII010000376 DAMZII010000000
VERSION     DAMZII010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.383"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..475
                     /locus_tag="POA77_005034"
     CDS             <1..475
                     /locus_tag="POA77_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HDI6368323.1"
                     /translation="RNEIQRFIRNGGEIKCISQLSDKEISSSYISLFHSRFGGTLPCY
                     EYDNLLMFISHLRELMFGHVLFWDNKPCAIDIVLKSESSCNVYYDVPNGAVLNDENCM
                     KLSPGSVLMWLNIDKARRYCQDNNKKMIYSIGAFRPEWKYKLLWSVPCKVGKCLC"
BASE COUNT          165 a           75 c          107 g          164 t
ORIGIN      
        1 ccgaaatgaa atccagagat ttatcaggaa tggcggagag attaagtgta taagccagtt
       61 atccgataaa gaaatatcca gctcttatat atcacttttt cattcccggt ttggaggtac
      121 cctaccttgc tacgaatatg ataatctgtt aatgtttatt tctcacttac gagaacttat
      181 gtttggccat gtgctattct gggataataa accgtgtgct attgatatag tactgaaatc
      241 tgaaagctca tgtaatgttt attatgacgt tccaaatggt gcagttctga atgatgagaa
      301 ttgcatgaaa ctaagtccgg gaagtgtcct tatgtggctg aatatagata aagcacgaag
      361 gtattgtcag gataataata aaaagatgat atattctatc ggtgcattca gacctgaatg
      421 gaaatataaa ctactatggt ctgttccctg taaggttggt aaatgcttat gttagaatat
      481 gtataaaggt aagtaactat ataagagcgt c
//
LOCUS       DAMZII010000377          507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMZII010000377 DAMZII010000000
VERSION     DAMZII010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.384"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            189..411
                     /locus_tag="POA77_005035"
                     /pseudo
     CDS             189..411
                     /locus_tag="POA77_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          176 a           75 c           87 g          169 t
ORIGIN      
        1 acgtttatta tgatgaatcc tctgcaaatg agcgaaattt gatagaacat ttatctgctg
       61 tacgatttaa tgaacttaat gatacagcat tacaaaaaca atcgaagttt ataaagatga
      121 ttcctgattg accagaaccc tatcctacaa tggtgactaa ttttattact cccgatggag
      181 atgatgatat gaatatatca tatgtgaata gcagcaaaac gacatcttta cctgttgagc
      241 tagatgcact aaataataaa gatatctctt atgccaaaga tttttcttat gctaaagatt
      301 tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga cctggagaag
      361 aagtatcaag ttctattgca agtaaagttt ttcacgcatt tattgattta gttaacaaaa
      421 tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc gaggaggtta
      481 aaggtgatta ttctcattac aggacct
//
LOCUS       DAMZII010000378          505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMZII010000378 DAMZII010000000
VERSION     DAMZII010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.385"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..395)
                     /locus_tag="POA77_005036"
     CDS             complement(<1..395)
                     /locus_tag="POA77_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6368324.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACML"
BASE COUNT          141 a          105 c          113 g          146 t
ORIGIN      
        1 agcatacagg cctcggaaga accaatggtg ttggtgccaa cggcctgacc atttttcggc
       61 gcaggcgcat gccacagatc ggcaaccata tttacgcaac gcaggtcgat ggctgcggat
      121 tgcggatatt cttctttgtc gatccagttt ttgttaatgg ataaatccat caatttgtgg
      181 acattttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc gttgccatca
      241 agatataatt cgtcattgat aatctggaat gcgacatcgt cgcgcatttc gtgcagcgga
      301 aaacgttttg attctgcgat agtggaaata gacttcgcac caaaacgtga atcgagtagt
      361 tccgacctta aatccgttac ttgcttctta tccattttaa actccttaaa atgattggat
      421 cgcattaaaa aagtaggatt tatcgataaa gtaagcaagt gcaaaggact cgtgtttaaa
      481 taacaaaatc ctaatgttat ttatc
//
LOCUS       DAMZII010000379          498 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMZII010000379 DAMZII010000000
VERSION     DAMZII010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.386"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>498
                     /locus_tag="POA77_005037"
     CDS             <1..>498
                     /locus_tag="POA77_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6368325.1"
                     /translation="FSVLMSLYCNEKPEFLNQCLISIHEQSVKPNEIIIVYDGYIPAE
                     LDNVVIEWSKVLPVKVCKLHTNMGLGDALNFGIKHCNNELIARMDTDDICAKDRFKLQ
                     LQYFNENPTLTLIGGGIEEYDEEMQVLRGTRFTKEKHADIVKYACFKNPFNHMTVMFK
                     KKDIQS"
BASE COUNT          179 a           71 c           93 g          155 t
ORIGIN      
        1 attctcagta ttaatgtctt tatattgtaa cgaaaaacct gaatttttaa atcaatgctt
       61 aataagtatc catgagcaat ctgtcaagcc taatgagatc atcattgttt atgatggcta
      121 tataccggct gaattagata atgtcgtaat agagtggagt aaagttttac cagtaaaagt
      181 ttgtaagtta cacacaaata tggggcttgg cgatgcactt aactttggta tcaaacattg
      241 taataatgaa ttgatcgcaa gaatggatac tgacgatatt tgtgctaagg atagattcaa
      301 attgcaactg caatatttca atgaaaaccc tactcttact ttaatcgggg gaggtattga
      361 agaatatgat gaggaaatgc aagtcctgag aggaacgcgt tttacaaaag aaaaacatgc
      421 tgatattgtc aaatatgctt gcttcaaaaa cccattcaat catatgactg taatgtttaa
      481 gaaaaaagat attcagtc
//
LOCUS       DAMZII010000380          489 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMZII010000380 DAMZII010000000
VERSION     DAMZII010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.387"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            78..>489
                     /locus_tag="POA77_005038"
     CDS             78..>489
                     /locus_tag="POA77_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368326.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTK"
BASE COUNT          164 a           73 c           96 g          156 t
ORIGIN      
        1 atcataattt ggtcatgaaa tttccgtctt gttacactac tctaaactag agcttatgga
       61 attagcgggt ggtattcatg gaaaataatg ataaattctt atcacaagac ttattggaat
      121 cttatgccat tcgtttgttg agcggaccat tgaatggatg cgagtatgaa atacttaatg
      181 ggcgtctact tgttatcatt ggtaatgatg tttcgttagg aagaagtgat gctttttctg
      241 agttaccaga aaacacaata gtcgttcctt atggcgagct tacaggtagt tttgagataa
      301 taattactac cgaccctgat ctggtagtaa cattcagaga attaacagca caagaacctg
      361 aagatagaac gttaacattg aatcagcaaa tagaagtttt aggtctcaaa tttgctgtaa
      421 aagaaaaaaa tgaagtttgg cagtattcat tgccaggcat tattgaaaat aacattattt
      481 ctacaaaac
//
LOCUS       DAMZII010000381          486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMZII010000381 DAMZII010000000
VERSION     DAMZII010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.388"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..446)
                     /locus_tag="POA77_005039"
                     /pseudo
     CDS             complement(1..446)
                     /locus_tag="POA77_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a          105 c           70 g          171 t
ORIGIN      
        1 agcttagttt tcagcctttt aacagcctcc aaggaagcat caagaactgg tgtctgattc
       61 ttcaaagctt atgaaaaact tttttgtgtt gattttcgag cttgttgttg agcagatttt
      121 cttaactctt tttcacgctt aacttgttct aaaagctctt tacggtcatc agaacgtgaa
      181 ccaaaaaatc tatcagcaag ttcagttaac aaattagata taatagtttt cagttctctt
      241 gcagcctggt ttcttatgaa tccctctctc cccgacttat ctttcagctc tttattactg
      301 gattgagtaa ttccaatata accaaaaatc cttctattag accaataata tcgccctgca
      361 ttccatgaac gtctttcttc tatctggaag aaatcattat ctactcgacc ataaggtaat
      421 actctcaagc tatccctaaa aatcattagt cctgcatact ttttggcctt aagatcaaag
      481 tgggaa
//
LOCUS       DAMZII010000382          483 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMZII010000382 DAMZII010000000
VERSION     DAMZII010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.389"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            118..>483
                     /locus_tag="POA77_005040"
     CDS             118..>483
                     /locus_tag="POA77_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368327.1"
                     /translation="MEGFNAPLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSH
                     SWENISIEDQDISQQIKALSFSRIDFECPKDNYQKIKKSISCLLIEIKITTSQIKKKH
                     LKARLVGSNVNIGFSGKYEE"
BASE COUNT          175 a           84 c           86 g          138 t
ORIGIN      
        1 tatttggaca gcctagatga aagaggccta atattgtctc tagcggcttt ctctgaagac
       61 gccttaggta aaatgctttt gacgttcatg ctcgataaca aagcgtctaa agaattaatc
      121 gaagggttta atgcccccct tggaacattt tcttcacgaa taaaagcctg tttttccttg
      181 ggactaataa cagagggtca atacaaagat cttgaacttc ttagaaaaat aagaaataaa
      241 ttctctcaca gctgggaaaa catttcaatt gaggatcagg atatttctca gcaaattaaa
      301 gcgttaagct tcagtcgtat tgattttgaa tgcccaaagg ataactatca aaaaataaaa
      361 aaatccatat cgtgtttact aattgaaatt aagataacca catctcaaat caaaaagaaa
      421 cacctcaaag caaggttagt tggttcaaat gttaacatag gattctccgg taagtatgag
      481 gaa
//
LOCUS       DAMZII010000383          480 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMZII010000383 DAMZII010000000
VERSION     DAMZII010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.390"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          170 a           69 c          101 g          140 t
ORIGIN      
        1 gaatgtggaa gtgtgaatgg cattaacgtg tgttttgaaa aaaatgaaat gataggttgg
       61 attaaaaaag aaatcccttt gctgtcgcaa aaaataggtg ggctttaaat ggtagggcaa
      121 tattcaaaga cagtataggt aactatttgt atggccatgt aaggtaaaag aaaaggaata
      181 aaccatgagc ggaaaaccgg cggcgcggca gggcgacatg acccgcaggg aaagatcatc
      241 agaagttcac tttttgtact aaataattcg cattttatgt ttaaaaattg agatattcct
      301 cattacctga agctgttttt tattgcttat acatgatcaa atactcctta cataaataag
      361 gtgaacaaat ggaacttaaa aaattgatgg aacatatttc tattattccc gattacagat
      421 aagcctggaa agtagagcat aaattgtcag gcatcctact attgactatt tttgccgtta
//
LOCUS       DAMZII010000384          476 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMZII010000384 DAMZII010000000
VERSION     DAMZII010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.391"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>476
                     /locus_tag="POA77_005041"
     rRNA            <1..>476
                     /locus_tag="POA77_005041"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAMZII010000385          474 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMZII010000385 DAMZII010000000
VERSION     DAMZII010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.392"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          144 a           95 c           98 g          137 t
ORIGIN      
        1 tttatttatt cccttttatg tcagaatatc aggggagtca gtcaaacccg gtatgattac
       61 tgccccggtc atccatgttc caggaaatat cagggcatac agggtgctgg taactgcgaa
      121 taaggccctg gtcggacact ttatgccggg aacgtctgta acatggaaac cggtaccttc
      181 ttcctgggtt tattttgtta actcttatct aaagggagac aaaatggcca aatcctgcga
      241 ttatgaagca atcaggggct ttattagcgt taatgtcggc aaagatacca ataacgccat
      301 tacagttaat tattcagata gcgtttgtgc gacaatgcat taccattcga cgaaaataaa
      361 ctcaggtggt tgatatcaga aatcaaccgt ggctccacct acaaaaaaag ttgctggttt
      421 ataaatccct cctttctgat taataagaaa actaataaca tatgagtgtg atta
//
LOCUS       DAMZII010000386          449 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMZII010000386 DAMZII010000000
VERSION     DAMZII010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.393"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          130 a           74 c          103 g          142 t
ORIGIN      
        1 aattctcatc atgaaatatg tttcctggtt tgtggcttgt aactggtcac ttctgaagtc
       61 gatctggaga ggcttgttga tgttggtgtt ttcaggatga tgtttcactt agtttgtttg
      121 ccgtatcgcc cggcgaatgg ctgtgattga ggaaggttta agtcgtagtg accaaagcta
      181 tatttaccaa cgaatgtaga tgaaaaaatc atctcctgcg ttccccatat ctccaggata
      241 aaaaggaatg taacaatctc atggcgtaag ctgacgaatc agccaggaat aatcgctagg
      301 gacctaagaa ttagcatgat aatagccact aagaaattac tgcgctccat gaaatagcca
      361 ttttgtggca aatggagttg actaataatg tcatatgtga gacggctagt tgaacgaata
      421 ttaaattttg ctgaattttt tatgttgat
//
LOCUS       DAMZII010000387          449 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMZII010000387 DAMZII010000000
VERSION     DAMZII010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.394"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            48..368
                     /locus_tag="POA77_005042"
                     /pseudo
     CDS             48..368
                     /locus_tag="POA77_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            complement(384..>449)
                     /locus_tag="POA77_005043"
     CDS             complement(384..>449)
                     /locus_tag="POA77_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDI6368328.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          130 a           81 c          100 g          138 t
ORIGIN      
        1 ttattccaca atacagaata ttttctttat tctttaaggt cgtttttatg atgaaaaatc
       61 cattagctta ttttcatatt gccacctttt tattgtcagg taaaagtcgt aatctgtcag
      121 aatactggaa agccgaagtc atcatgcggg ataagtttgt cttacaccgc ctgataagaa
      181 acgggttgca gagacaacga ggctttctga tgtggtggcg cctggccaat gagatgttta
      241 tctctggaaa ccaaaaacaa aggaaatgtg cgataaagat aaaaaatgca cttatggaac
      301 ggtatggatg tgatatcggc ctgggcgcgc ggattggtaa aggcctcatg ttacctcatc
      361 atgcaggagg taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg
      421 actttgtcat gcagctccac cgattttga
//
LOCUS       DAMZII010000388          419 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMZII010000388 DAMZII010000000
VERSION     DAMZII010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.395"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>419)
                     /locus_tag="POA77_005044"
     CDS             complement(<1..>419)
                     /locus_tag="POA77_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI6368329.1"
                     /translation="KPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKHCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYD
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRF"
BASE COUNT          126 a           91 c           66 g          136 t
ORIGIN      
        1 taaatctatc gcctgtatga tccccaagga agatacaagg ggttatcgca tcgtccttta
       61 aaaaaacatg ttgatgcaac tcattcaaga tcatgtctct cgtatttaat aaatcgtaga
      121 gcactttatt atttttatgt ttgtatgcag atgaagccat ctcttcgtgt tttaataatt
      181 cacataataa actccaaccc ttttccctga tgcctatatg ccctgaaacg agcaatgcat
      241 aaagtacagc gcctactctt ccatctgtat cgccaaaata agccgtgttg gcattttgtg
      301 ggttaatcac accacaatgt ttatagaagt ctatttttga aaacacaggt ttaacatagc
      361 tatcaaactt ttccttagta aacgtctctt tagagacatc ccaacgccag tcaggtttg
//
LOCUS       DAMZII010000389          408 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMZII010000389 DAMZII010000000
VERSION     DAMZII010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.396"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          126 a           68 c           80 g          134 t
ORIGIN      
        1 cactgagtta gcttgcaaat ctttccttgt ttatacttta aataaattac ttataaaaca
       61 gataattaaa caacaatctt tggtttgtga aggcctccgc aagtgttcgt ctggcttcac
      121 atggcatctt cttcttagaa aaatatcgac atattttgtg acacgaattg caaatctggt
      181 tttgttgtat ggattgcgtg atttttgatc tggtataaca ggtataaagg tgcgccaaga
      241 tagtcaatga gacagggcat ctcgcaatct atggcaaaca tcacttcagt tctttctcat
      301 cgggtgatga aaacgcactt cagtctgaaa ggaatatgaa aatgagatca acagacattc
      361 tattttatga ctctgggtaa aatggattga gtaagtgata tagcttac
//
LOCUS       DAMZII010000390          407 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMZII010000390 DAMZII010000000
VERSION     DAMZII010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.denovo.397"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..305)
                     /locus_tag="POA77_005045"
     CDS             complement(<1..305)
                     /locus_tag="POA77_005045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368330.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     "
BASE COUNT          100 a           96 c          107 g          104 t
ORIGIN      
        1 gtacaaaaag tgtacttctg atgatctttc cctgtataca tccggtctgc ggggctgtgt
       61 gccaggcggt catcgtcggt atcgccggtc acctggtggc gcagacgcat tcccggaaag
      121 gcgtcataaa agaagcggca gctcagggca tcacggtcga acagcaccag gtaatcatcg
      181 cggcggcgca ggatgtactg ggcgtgatac gggttactgg tttcgatatt ggctaacatc
      241 cgctgtttta ttaatgcgat atctaaaatc aggatggaaa tagtgatgta tatcactatt
      301 tccatgatct tgccttgcgt tcatccaggc aggaatatcc ctgttagcag cctctgtgcg
      361 atgtacgcca gcggtctgta aagaaaccga ttaccttata acaaaac
//
LOCUS       DAMZII010000391         7084 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.199, whole genome shotgun sequence.
ACCESSION   DAMZII010000391 DAMZII010000000
VERSION     DAMZII010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.199"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..954
                     /locus_tag="POA77_005046"
     CDS             <1..954
                     /locus_tag="POA77_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6368331.1"
                     /translation="HPYAYVADTTGLDEKTVRDIFNARAEFLGRWHRFETPRILGIDE
                     LYLNKRYRCILTNIEERTLLDLLATRRQDVVTNYLMKMKDRQKVKIVSMDMWNPYRAA
                     VKAVLPQARIVVDKFHVVRMANDALEKVRKGLRKELMPSQSRTLKGDRKILLKRAHEV
                     SNRERLIMETWTGAFPQLLAAYEHKERFYGIWDATTRPQAEAALDEWIATIPKGQKEV
                     WSDLVRAVGNWREEIMTYFETDMPVTNAYTESINRLAKDKNREGRGYSFEVMRARMLY
                     TTKHKKKAPTAKVSPFYKKTIGYGLPDFAEELNYGVDLSTI"
     gene            complement(1016..1639)
                     /gene="tetR(H)"
                     /locus_tag="POA77_005047"
     CDS             complement(1016..1639)
                     /gene="tetR(H)"
                     /locus_tag="POA77_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDI6368332.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            1731..2933
                     /gene="tet(H)"
                     /locus_tag="POA77_005048"
     CDS             1731..2933
                     /gene="tet(H)"
                     /locus_tag="POA77_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDI6368333.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLHIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            complement(2947..4878)
                     /locus_tag="POA77_005049"
     CDS             complement(2947..4878)
                     /locus_tag="POA77_005049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6368334.1"
                     /translation="MEIRSPLIHLALLESLKANEISDEIDLFLPFIAVTLSELPGEEV
                     TPAVLQEKFTQSFGITPPISAIQVFMTRAKKRKLLHRENHVFIPNSSQVDKWKNGYHD
                     KKDDIETSLEILRNDFKEFANDHFGKSITDNECDILITEFIDKNISSVTTHNRFEKGQ
                     LKSKIKNTDHVTASFISHIHKTKSSTLDHFSRIVKGMVLANYLCLADKIGSKKKFNSI
                     TAYLDTPIIIGMLGFSGSQRKKSINEFISLLKKVGIELRLFDKSLDEAEMLLGAWRDD
                     LRKRNYRKFNSKTLELLRSQGYDAERLDTEIKLLKSRIEKESITLVSGFRTNPRYQCD
                     EKALESAISTNFKDSKNLEHDTICISRIYNLREGKLIGDLNQKMAIFVTPNTGLVNHA
                     NEYFKNEIPTKKYIPLVVSEQWMTAIFWLKSPEIFNTLPMDQIIASAYGLLYTDDKFW
                     NSFIDKLQLLESKGQITEDDFTVVRWDSDLLGLIHDVSVDVGENFTEKDVFEIVEKIK
                     NKHTQEKEAEITQIKNEKDFEIKELETEIASKDKQIINTQVKIRNISNGIGIATSGLF
                     CLLLMSCVFWTAYTSLPIDMVNYSIIQTYKKSFLTISALFITSVFSFMGTMFGWNVIS
                     VYKWIKNKVSTFVYELLIG"
     gene            complement(4878..6002)
                     /locus_tag="POA77_005050"
     CDS             complement(4878..6002)
                     /locus_tag="POA77_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6368335.1"
                     /translation="MNLFLGADNVENGTLSLDKTTFISKEKHESMRKGVVQEKDILLV
                     MRGNGTGRSCIYENNAKSYAARINSGLVIIRANSAHTDIQFFQQLVLSPVIQKQFESF
                     MFGSAQPQLTIQLLKSLRFPFPPLPEQKKIAQILSTWDKVIATTEQLLANSQQQKKAL
                     MQQLLTGKKRLLDKNGVRFSGEWKSYHLSHVTVIVMGSSPKSEAYNENGIGLPLLQGN
                     ADIKNRISAPRVFTSEITKECEIGDILLSVRAPVGTVAISEHKACIGRGIAALHAKPN
                     FDQSFIYQFLLWFEPKWGSLSQGSTFESINSDDIKNLKIKMPNFGEQQKIAAVLSTAD
                     QEISALQQKLDALKQEKKALMQQLLTGKRRVTIEKKELLK"
     gene            6093..7064
                     /locus_tag="POA77_005051"
     CDS             6093..7064
                     /locus_tag="POA77_005051"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6368336.1"
                     /translation="MSNITFPIALWEGFEAVHTEQNQQTLIIHLKPIDNGHCACGKPA
                     KAVHDASLRSVSERTILAFQVQLRLPVRRILCPDCGIVREHISWLKPYARQTTRLIEH
                     VEALLKLLPIKHISQLLGLHWHTIKTIDKRRLAREVTEPDWSRVKRLVMDEFALFKGH
                     RYATVVADADTHQVLWIGEGRSREAIRPFFVKLGQYCQQIEAVAMDMNTAFDLEVQKH
                     CPQAKVVYDLFHVVAKYGREVIDRVRVDQANQLKHDKPARKRVKRGRWVLLKNRDNLT
                     DKQAGYLNELLESNKSLMTVYLLREQLKEMWYCTDEAEATAQCEVVY"
BASE COUNT         1901 a         1586 c         1516 g         2081 t
ORIGIN      
        1 cacccctacg cctacgtggc tgataccacg ggcctggacg agaagacggt acgcgacatt
       61 ttcaacgccc gcgccgaatt cctggggcgc tggcaccgct tcgagacgcc ccgcattctc
      121 ggcattgacg agttgtacct gaacaagcgc taccgctgca tcctgaccaa catcgaggag
      181 aggaccctgc tcgacctgct ggccacccgc cgtcaggacg tggtgaccaa ctacctgatg
      241 aagatgaaag accggcagaa ggtcaagatc gtcagcatgg acatgtggaa tccctaccga
      301 gcagcggtca aggcggtgct gccacaggcc cgcatcgtgg tcgataagtt ccatgtggtg
      361 cgcatggcca acgatgccct ggagaaggtg cgcaagggcc tcaggaagga gctgatgccc
      421 tcccagagcc gaaccctcaa gggtgaccgg aaaatcctgc tgaaacgcgc tcacgaagtc
      481 tcaaaccggg aacgcctcat catggagacc tggaccggcg cattcccaca actgctggcc
      541 gcctacgagc acaaggaacg tttctacggc atctgggacg ctaccacacg gccccaggca
      601 gaagccgccc tggacgagtg gatagccacc atcccgaagg gccagaagga agtctggagc
      661 gatctggtca gggctgtagg caactggcgc gaagagatca tgacctactt cgagacggac
      721 atgcccgtca ccaacgctta cacggagtcc atcaaccgac tggccaagga caagaaccgt
      781 gaaggacgtg gttactcctt cgaggtgatg cgggcacgaa tgctctacac cacgaagcac
      841 aagaagaagg ccccgaccgc gaaggtctct ccgttctaca agaaaaccat cggttacgga
      901 ctgccggact tcgcagagga actcaactac ggggtcgatc tatcaaccat ctgataatgg
      961 tatcaggttg gtggggtaaa ggtgccccat caaccattaa atccgtatac ccgatttatt
     1021 tagcgctctt taatactgtt tcaagtccag agatcatcac atcaaggaca aacaaaaatg
     1081 cagcatcacc attatcacta tccataattg caacggcttg ggttaataat ggcggatagg
     1141 caacagtatc cgtctctact ttttcacgct ctttttggct ttcttgatgc tcttgagttt
     1201 ccagtacgga gcctaatgta aaatgcgcaa tagagcttaa tgcatacacg gcttgagata
     1261 gactaaaccc agcatcacac aaaaactgta gttgctgttc tgatgtctca aattgacttt
     1321 cagaggggcg tgttcccgca tgaattttgc caccatcacg atacattaat aaggcttggc
     1381 ggaagctttt cgcgttattt cgcaaaaagt cctgccatgt ttcattcggc aatggcaaaa
     1441 catgatgatg gtgcttttgc aaaatagttt ctgctaatgc atctaacaaa gcgcgtttat
     1501 tttttacatg ccaatacaat gtgggttgtt ccacacctat tttttgcgcc agcttacgcg
     1561 ttgttaatcc ttcaatacca acttcattaa gtaaaatcaa cgcattatca ataacttgtt
     1621 ctttatctag ctttgccatt acctacctca aaataaaata gccttgacaa tctatcactg
     1681 atagagatat tttacaccca ctctatcact gatagatttt taggatctca atgaataaat
     1741 caattattat tatactgctg atcaccgtat tagatgccat tggtatcggg cttatcatgc
     1801 cagtactccc tactctatta aatgaatttg tcagtgaaaa ttcactggca acccattacg
     1861 gtgtgctatt agcgctctat gctaccatgc aggttatttt tgctcctatt ctaggacgac
     1921 tgtctgataa atacggcaga aaacccatct tgctgttttc ccttttaggc gcggcactcg
     1981 actatctttt aatggcattc tcaaccacac tttggatgct ctatattggg cgcatcattg
     2041 cggggatcac aggcgcaaca ggtgccgtat gtgcatcagc gatgagtgat gtgactcccg
     2101 ctaaaaatcg aactcgctat tttggtttct taggtggtgc ttttggtgtt ggccttatta
     2161 tcggcccaat gctaggggga ttattaggtg atatcagtgc tcatatgcca tttatttttg
     2221 ccgctatttc acactcgata ttattaatac tctctttgct ctttttccga gaaacacaaa
     2281 aaagagaagc gcttgttgcc aataggacac ctgaaaacca aactgcctca aatacagtca
     2341 ctgttttttt taagaaaagc ctctactttt ggttagcaac ctattttatt atccagctta
     2401 tcgggcaaat tcctgccacc atctgggtgc tgtttacaca atatcgtttt gattggaaca
     2461 caacttctat cggtatgtct ttggcggttc tgggtgtatt acatattttc tttcaggcga
     2521 ttgtcgctgg gaaattggca caaaaatggg gcgaaaaaac caccattatg atcagtatgt
     2581 ctattgatat gatgggctgt ttattattag cgtggatagg ccacgtttgg gtcatcttac
     2641 cagcattaat ttgcttagcg gcaggaggta tggggcaacc cgcattacaa ggttatttat
     2701 caaaatctgt cgatgataat gcgcaaggga aattacaagg tactctggtg agcctaacca
     2761 atattaccgg gatcattggt ccccttttat ttgcctttat ttatagttat agcgtcgctt
     2821 attgggatgg tctgttatgg ctgatggggg caatacttta tgctatgttg cttattaccg
     2881 cttattttca ccaaagaaaa accacaccta aagctgttat ttcaacccct taatactaga
     2941 aattaattag cctataagta actcgtatac gaatgtggag actttgttct ttatccactt
     3001 gtaaacggag attacgttcc acccaaacat agtgcccata aagctgaaaa cgctcgtgat
     3061 gaataatgcg gagattgtta ggaaactttt cttatacgtc tgaatgatcg aatagttgac
     3121 catatcaata ggtaggctag tgtaagcagt ccaaaagaca catgacatta ataataagca
     3181 gaacaaccca gatgtggcga tccctatacc attagaaata ttccgtatct ttacttgggt
     3241 gttgataatc tgcttgtctt ttgaagctat ttcagtctct agctctttaa tttcgaaatc
     3301 tttttcattt tttatttgcg taatctcggc ttctttctcc tgggtatgct tattctttat
     3361 cttttctact atttcaaata cgtctttctc tgtgaaattc tcaccaacat cgacagagac
     3421 atcatgtatc aaacctaata aatccgaatc ccatcgaaca actgtaaagt cgtcttcagt
     3481 aatttgccct ttactttcaa gtagttgcaa tttatcaatg aatgagttcc agaacttatc
     3541 gtccgtgtat aggagaccat aagccgaagc aataatctga tccattggaa gtgtattgaa
     3601 gatttctgga cttttaagcc agaatatagc tgtcatccat tgctcagata caaccagagg
     3661 gatatatttc tttgtcggta tttcattttt gaaatactca tttgcatgat tgactaggcc
     3721 cgtatttggc gtaacaaaaa tagccatttt ttgattgaga tcacctatga gcttcccttc
     3781 tctaaggttg tagattctag agatacaaat agtgtcatgc tcaagattct tgctatcttt
     3841 aaagttggta gatattgctg actctaatgc cttttcatca cactgatacc ttggattggt
     3901 tctgaatcca gaaactaacg ttatactttc cttctcaatc ctagatttaa gaagttttat
     3961 ctcggtatct aatcgctcgg catcgtatcc ttgtgatcta agcaattcca gcgtcttaga
     4021 attgaatttt ctatagtttc tttttcttaa gtcatctctc caggcaccaa gaagcatttc
     4081 agcctcatca agagatttat caaatagacg caattcgatt ccgactttct ttaaaagaga
     4141 aataaattca ttaatggact tctttctttg gctgccacta aaccctaaca tgccaataat
     4201 tatgggcgta tcaagataag cagtaattga gttgaatttc ttcttgctgc caatcttatc
     4261 tgcaaggcag agataattgg ctaaaaccat gcctttgacg attcttgaaa agtgatccag
     4321 tgtggatgat ttggttttat gtatatggct tatgaatgat gctgtaacat gatcggtatt
     4381 tttaatcttt gatttgagct ggcctttctc aaacctgttg tgagtggtaa cggatgatat
     4441 attcttatca ataaactctg ttatgagaat atcacactca ttgtctgtta ttgattttcc
     4501 gaaatggtca tttgcaaatt ctttgaagtc atttctcaat atttcaagag atgtttcaat
     4561 atcatccttt ttgtcatgat atccattttt ccatttatca acttgactgc tgttagggat
     4621 gaagacgtgg ttttctctgt gaagtaactt cctttttttt gccctggtca tgaaaacttg
     4681 tattgcagaa attggcggtg taataccaaa agactgtgta aatttttcct gcaaaacagc
     4741 gggggtaact tcttcacctg gaagttcaga taaagtaaca gcaataaatg gcaaaaatag
     4801 gtctatctca tcagatattt cgtttgcctt taatgactca agcaatgcaa ggtgtataag
     4861 tggacttctg atttccatta ctttaaaagt tccttcttct cgatagttac gcggcgttta
     4921 cccgttaaca gctgctgcat cagtgccttt ttctcttgct ttaaggcatc gagcttttgt
     4981 tgcaaagcag agatttcctg atcggcagtg gaaagtacgg cggcgatttt ttgttgctcg
     5041 ccaaaattag gcatcttaat cttcagattt tttatgtcat cactatttat tgactcgaag
     5101 gtgcttccct gggaaagaga gccccacttc ggttcgaacc aaaggaggaa ttgatagata
     5161 aagctttggt caaaattagg cttggcatgc aatgcggcta ttcctcgccc aatacaagct
     5221 ttatgctcag atatagcgac ggttcctaca ggggctcgaa cacttagtaa tatgtcaccg
     5281 atttcacact cttttgtgat ttcagaagta aaaactcttg gggccgaaat gcgatttttt
     5341 atatctgcat ttccttgaag taaaggaaga ccaattccat tttcgttgta tgcttcagat
     5401 ttaggtgacg aacccatcac aattacagtc acatgagaca aatggtagga cttccactcc
     5461 ccactaaacc gaaccccatt cttatccagc aggcgttttt tgccggtgag cagttgttgc
     5521 atcagggctt ttttttgttg ctggctgttg gcgagcagtt gttcggtagt tgcgatgacc
     5581 ttatcccagg tagagaggat ttgagcgatt ttcttttgtt cggggagtgg gggaaatgga
     5641 aagcgcaagc ttttcagaag ctgaatagtt aattggggct gtgcactccc aaacataaag
     5701 ctttcgaatt gtttctgaat gacgggcgac aagaccaact gctggaagaa ctgaatatct
     5761 gtgtgtgcag aattagctct gattataact aaaccagagt ttatacgtgc tgcatatgac
     5821 tttgcgttgt tctcatagat gcatgaacga cccgttccat tccctctcat tacaagaagt
     5881 atgtcctttt cttgaacaac gcccttacgc attgattcat gcttttcttt agatatgaaa
     5941 gtggttttgt ctaaagataa agtaccattt tcaacattat ctgcccctaa aaaaaggttc
     6001 atcgcagatt agcgtggact ggaaatgaaa acggttggag agttattgtt tagtcgccaa
     6061 acaaaaacaa tgaccaaccc caaccgttct caatgtcgaa tattaccttc cccattgcgc
     6121 tctgggaagg ctttgaagcc gtccataccg agcaaaatca acaaaccctc atcatccacc
     6181 taaagccgat tgataacggc cactgtgctt gcggcaaacc ggctaaggct gttcacgacg
     6241 cttcacttcg cagcgtatcg gaacgaacca tactggcgtt tcaggttcag ttacggctac
     6301 ccgtccgccg tattctttgc cctgattgcg gtatcgttcg ggagcatatc agctggttaa
     6361 agccctatgc tcgccagacc acacggctca ttgagcatgt cgaggcgcta ctcaagctct
     6421 tgccgattaa acatatctcg caactccttg gcctgcactg gcataccatc aagactatcg
     6481 acaaacgtcg gctggcaagg gaggtcacag agcctgattg gagtcgtgtc aaacgcctgg
     6541 tcatggacga gtttgccctg ttcaaaggcc atcgttacgc caccgtggtg gccgatgcgg
     6601 atactcatca agtcctttgg ataggtgaag gccgcagtag agaagctatc cggccgtttt
     6661 tcgttaagtt ggggcagtac tgccaacaga ttgaagccgt tgctatggat atgaatactg
     6721 cctttgacct ggaggtacag aagcactgtc cccaagccaa agtggtctac gacttgtttc
     6781 atgttgttgc caagtatggc cgtgaagtca tcgaccgagt cagggttgac caggctaatc
     6841 aactcaagca tgacaaaccg gcccgcaaac gggtaaaacg ggggcgctgg gtcttactca
     6901 aaaacagaga taatttgaca gacaaacagg cggggtatct caacgagctg ttggagtcga
     6961 acaaaagctt aatgacggtt tacctgctca gggagcaact caaggagatg tggtactgca
     7021 cagatgaagc cgaagccacg gctcaatgtg aagtggttta ctgaatttgg ccacctgaac
     7081 agag
//
LOCUS       DAMZII010000392         6340 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.212, whole genome shotgun sequence.
ACCESSION   DAMZII010000392 DAMZII010000000
VERSION     DAMZII010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.212"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(97..1074)
                     /gene="epmA"
                     /locus_tag="POA77_005052"
     CDS             complement(97..1074)
                     /gene="epmA"
                     /locus_tag="POA77_005052"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDI6368337.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1399..3207
                     /gene="frdA"
                     /locus_tag="POA77_005053"
     CDS             1399..3207
                     /gene="frdA"
                     /locus_tag="POA77_005053"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDI6368338.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3200..3934
                     /gene="frdB"
                     /locus_tag="POA77_005054"
     CDS             3200..3934
                     /gene="frdB"
                     /locus_tag="POA77_005054"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDI6368339.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3945..4340
                     /gene="frdC"
                     /locus_tag="POA77_005055"
     CDS             3945..4340
                     /gene="frdC"
                     /locus_tag="POA77_005055"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDI6368340.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4351..4710
                     /gene="frdD"
                     /locus_tag="POA77_005056"
     CDS             4351..4710
                     /gene="frdD"
                     /locus_tag="POA77_005056"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDI6368341.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4773..5906
                     /locus_tag="POA77_005057"
     CDS             4773..5906
                     /locus_tag="POA77_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI6368342.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            5995..>6340
                     /gene="blc"
                     /locus_tag="POA77_005058"
     CDS             5995..>6340
                     /gene="blc"
                     /locus_tag="POA77_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDI6368343.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGG"
BASE COUNT         1565 a         1655 c         1662 g         1458 t
ORIGIN      
        1 cttaaattgt ggcctttaac ggaaaaatga aaacatatgc tacagaatgg cgcttatcac
       61 gccattcttc gctgttaatt cagtaatttt tcagaattat gcccggtcaa cgctaaaggc
      121 gatgacttca gccagtgtct ccgcgcccag cgccaacatc accagacgat caacacctaa
      181 tgccacgccg gaacagtcag gcataccgac tttcaaggct tcaatcagat tctggtcaat
      241 ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta tcttgttcaa agcgttgttg
      301 ctgctcacgg gcatccgtca attcatggaa accattcgcc agctcaatac ctttataata
      361 aacctcaaag cgttcagcga cccgatgatc ttcggtactg atttgcgcca gtgatgcctg
      421 gctggctgga aagtggtaca caaaggtcgg tttttctttg ccaatatttg gctctacgcc
      481 aaaggtaaac agcaattgta gcagcgtgtc gcggtcttct tcggtatcag caacattgct
      541 caaatccagt ttcgctgcga cttcccgcag ttgcgttttg tcggcagaga gcgggtcaat
      601 ttccagataa cgcaagaaag cttgttgata agaaaggctt tctgctgccg ggcagtccag
      661 cacctgttgt aagagatcgt ccacctcgtt catcaaccgg tacatatcat agtgcggtcg
      721 ataccactcc agcatagtga actcagggtt gtgataacgc cccatctctt cattacggaa
      781 gctgcggcac agctggaata ccggcccaca accggcaacc agcaggcgtt tcatatggta
      841 ttccgggctg gtcattaacc agagattcat cccctgcgaa tgcccggggc caacgaaacg
      901 tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc tggctcatac aaggcgtctc
      961 cacctccagc actccacgat cggcaaagaa acgacggatc tccgccataa tcgccgcgcg
     1021 ttttaataag ttaggaatgg atgcgctcgg ctgccaggat gccgtttcgc tcatagttaa
     1081 atctccagtt tttgacaagg gcacgaagtc tactcgcaac gcgacggcga gacaaatttt
     1141 acgcaggaat caaacagcgg tgggcagtga ctaaaaaaag cacgatctga tggtttagta
     1201 attaaattaa tcatcttcag tgataattta gccctcttgc gcactaaaaa aatcgatctc
     1261 gtcaaatttc agacttatcc atcagactat actgttgtac ctataaagga gcagtggaat
     1321 agcgttcgca gaccgtaact ttcaggcact taccctgaag tacggggctg tgggataaaa
     1381 acaatctgga ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt aggcgccggt
     1441 ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca
     1501 ctaatctcaa aagtataccc gatgcgtagc cataccgttg ctgcagaagg gggctccgcc
     1561 gctgtcgcgc aggatcatga cagcttcgaa tatcactttc acgatacagt agcgggtggc
     1621 gactggttgt gtgagcagga tgtcgtggat tatttcgtcc accactgccc aaccgaaatg
     1681 acccaactgg aactgtgggg gtgcccatgg agccgtcgcc cggatggtag cgtcaacgta
     1741 cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg ccgccgataa gaccggcttc
     1801 catatgctgc acacgctgtt ccagacctct ctgcaattcc cgcagatcca gcgttttgac
     1861 gaacatttcg tgctggatat tctggttgat gatggtcatg ttcgcggcct ggtagcaatg
     1921 aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg cggtcgttat ggctactggc
     1981 ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca tcgttaccgg tgacggtatg
     2041 ggtatggcgc taagccacgg cgttccgctg cgtgacatgg aattcgttca gtatcaccca
     2101 accggtctgc caggttccgg tatcctgatg accgaaggct gccgcggtga aggcggtatt
     2161 ctggtcaaca aaaatggcta ccgttatctg caagattacg gcatgggccc ggaaactccg
     2221 ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt ttctcaggcc
     2281 ttctggcacg aatggcgtaa aggcaacacc atctccacgc cgcgtggcga tgtggtttat
     2341 ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa
     2401 ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac cgattccggt acgtccgacc
     2461 gcacactaca ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg cattaaaggt
     2521 ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg tctgggctcc
     2581 aactccctgg cggaactggt ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt
     2641 gcagcaactg ccggtaatgg caacgaagcg gcaattgaag cgcaggcagc tggcgttgaa
     2701 caacgtctga aagatctggt taaccaggat ggcggcgaaa actgggcgaa gatccgcgac
     2761 gaaatgggtc tggctatgga agaaggttgc ggtatctacc gtacgccgga actgatgcag
     2821 aaaaccatcg acaagctggc agagctgcag gaacgcttca agcgcgtgcg catcaccgac
     2881 acctccagcg tgttcaacac cgacctgctc tacaccattg aactgggcca cggtctgaac
     2941 gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag
     3001 cgtctggacg aaggttgcac cgagcgtgac gacgtcaact tcctcaaaca caccctcgcc
     3061 ttccgcgatg ctgatggcac gactcgcctg gagtacagcg acgtgaagat tactacgctg
     3121 ccgccagcta aacgtgttta cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc
     3181 aataagaagg agaaggcgaa tggctgagat gaaaaacctg aaaattgagg tggtgcgcta
     3241 taacccggaa gtcgataccg caccgcatag cgcattctat gaagtgcctt atgacgcaac
     3301 tacctcatta ctggatgcgc tgggctacat caaagacaac ctggcaccgg acctgagtta
     3361 ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc
     3421 aaaactggca tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt
     3481 agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca tcgaaagtct
     3541 ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg gtactaacat
     3601 ccagaccccg gcgcagatgg caaagtatca ccagttctcc ggttgcatca actgtggtct
     3661 gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc cggctgccat
     3721 tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg agcgtatggc
     3781 gcagttgaac agccagaacg gcgtatggag ctgtactttc gtgggctatt gctccgaagt
     3841 ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag ggcaaagtag aaagttcgaa
     3901 agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg actaaacgta
     3961 aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt tatcgctttt
     4021 acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg
     4081 ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac tttttacaaa
     4141 acccggttat cgtgatcatt aacctgatca ctctggcggc agctctgctg cacaccaaaa
     4201 cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa aaaatgggac
     4261 cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc gtaatcctgt
     4321 ttgttgccct gtactggtaa ggagcctgag atgattaatc caaatccaaa gcgttctgac
     4381 gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg
     4441 gtgatgatcc tgctggtggg tattctgctg ccactggggc tgtttccggg tgatgcgctg
     4501 agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg
     4561 atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat gcacgatctg
     4621 aaaattcacg tgcctgcggg caaatgggtt ttctacggtc tggctgctat cctgacagtt
     4681 gtcacgctga ttggtatcgt tacaatctaa cgtatcgcca atgtaaatcc ggcccgccta
     4741 tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa aacgacgctc tgcgccttat
     4801 taattaccgc ctcttgctcc acatttgctg cccctcaaca aatcaacgat attgtgcatc
     4861 gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg gcggtaattt
     4921 atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa aagcagcccg
     4981 tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg
     5041 gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccacaaca aaatactggc
     5101 ctgaacttac cgctaaacag tggaatggga tcacactatt acatctcgca acctacactg
     5161 ctggcggcct gccattgcag gtgccggatg aggtgaaatc ctcaagcgac ttgctgcgct
     5221 tctatcaaaa ctggcagcct acatgggcgc caggaacaca acgtctgtat gccaactcca
     5281 gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt gagcaggcga
     5341 tgcaaacacg tgtcttccag ccactcaaac tcaaccatac gtggattaat gtacctcccc
     5401 cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa ggcagtgcat gtttcgccag
     5461 gggcgttaga tgctgaagct tatggtgtga agtcgaccat tgaagatatg gcccgctggg
     5521 tacgaagcaa tatgaatccc cgtgatatca acgacaaaac acttcagcaa gggatacaac
     5581 tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc tgggaaatgc
     5641 tggactggcc ggtaaatcct gacagcatca ttaacggcag tggcaataaa attgcactgg
     5701 cagcacaccc tgtaaaagcg attacgcccc caactcctgc agtacgcgca tcatgggtac
     5761 ataaaacagg ggcaaccggc ggatttggta gctatgtcgc gtttattcca gaaaaagagc
     5821 tgggtatagt gatgctggct aacaaaaact atcccaatcc agcgagagtc gccgccgcct
     5881 ggcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg gaccttttct
     5941 gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac atttatgcgc
     6001 ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg cagttctcct
     6061 acgccgccgc gtggcgtaac agtagtgaat aatttcgacg ccaaacgtta tctcggtacc
     6121 tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa agtcaccgca
     6181 acatacagcc tgcgtgatga cggcggcctg aatgtcatta ataaaggcta taaccctgac
     6241 agaggaatgt ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc aactcgcgct
     6301 gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
//
LOCUS       DAMZII010000393         4371 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.238, whole genome shotgun sequence.
ACCESSION   DAMZII010000393 DAMZII010000000
VERSION     DAMZII010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.238"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..256
                     /gene="prfH"
                     /locus_tag="POA77_005059"
     CDS             <1..256
                     /gene="prfH"
                     /locus_tag="POA77_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDI6368344.1"
                     /translation="GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWK
                     LEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(313..1770)
                     /gene="pepD"
                     /locus_tag="POA77_005060"
     CDS             complement(313..1770)
                     /gene="pepD"
                     /locus_tag="POA77_005060"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDI6368345.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2031..2489
                     /gene="gpt"
                     /locus_tag="POA77_005061"
     CDS             2031..2489
                     /gene="gpt"
                     /locus_tag="POA77_005061"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6368346.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2581..3825
                     /gene="frsA"
                     /locus_tag="POA77_005062"
     CDS             2581..3825
                     /gene="frsA"
                     /locus_tag="POA77_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDI6368347.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3883..4284
                     /gene="crl"
                     /locus_tag="POA77_005063"
     CDS             3883..4284
                     /gene="crl"
                     /locus_tag="POA77_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDI6368348.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         1065 a         1081 c         1142 g         1083 t
ORIGIN      
        1 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
       61 tagcgtgaag gttcagtcag agcgtagtca gcatgctaac aagcggctgg cacgattgct
      121 gattgcctgg aagctggagc agcagcaaca ggaaaatagc gcggtgctga aatcgcagcg
      181 gcgaatgttc catcaccaga ttgaacgtgg caacccgaga cggacattta cagggatggc
      241 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
      301 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta
      361 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
      421 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
      481 cgcgtggata atctggatgt tcggcgtttt gttgaacaga cgctgatagg tttcacgtac
      541 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
      601 ggttttcgcg ccagccagtt tacccagcga atccagcatg ctcacgacgt agtctttacc
      661 gctgtcgatc agtgaacgga tcagacagtg aatttctacg ttattatcag tcatggtcac
      721 cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtctgagt tgcggatcac
      781 accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
      841 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag
      901 ctcgtttttc aggatctcct gataggtatt caccagagat ttcaggacgt cgactttatc
      961 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc
     1021 gttgaaatcg ataaggcgca gatccagttc ttctgcatga cccgccagga agcgcaccag
     1081 cagtttgttg gcattaccca ggccaacgtg gatttccccg ccggagtgac cgccttttag
     1141 accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg
     1201 caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc
     1261 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
     1321 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt
     1381 ttcgtcagcc agaaccgcca gcgcagacgc cataccaatg ccgttatctg cacccagcgt
     1441 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
     1501 gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac
     1561 cggtttacga ttttccatac ctgcggtagc aggtttacga atcaggatat tacctacctg
     1621 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
     1681 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
     1741 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
     1801 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
     1861 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     1921 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     1981 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     2041 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     2101 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg
     2161 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     2221 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     2281 tcatcgttat tgatgaccta gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     2341 atccaaaagc acattttgtg actatcttcg caaaaccggc tggtcgtccg ctggttgatg
     2401 actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg
     2461 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     2521 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     2581 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     2641 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggctcttgat
     2701 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     2761 attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga tgctgaacgt
     2821 accgatgatg atttatacga taccgtgatt ggctaccgtg gtggcaactg gatttatgag
     2881 tgggccaccc aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc
     2941 agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg
     3001 aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta tgaagaggcc
     3061 gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc
     3121 atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt attaatgtgt
     3181 ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg
     3241 cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc aaaatggaag
     3301 ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg
     3361 tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg
     3421 cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt
     3481 cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt
     3541 ctggcgagtc gtttggggat gcatgatgct tccgacgaag cgttgcgcgt ggagttgaat
     3601 cgctattcat taaaagtgca aggattgctg ggacgtcgct gcccaacgcc aatgttatca
     3661 ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca
     3721 tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa ttttgacaaa
     3781 ggtcttcagg aaatcaccga ctggatcgaa aaacgtttgt gttaaaaatt tgctaaattt
     3841 taccaatttg gtaaaacagt tgcatcacaa caggagattg caatgacgtt accgagtgga
     3901 cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt
     3961 aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg
     4021 gcaccggaag tgcgtgagtt ctggggctgg tggatggagc tggaagcgca ggaatcccgt
     4081 tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg
     4141 gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag
     4201 ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcggatga ttttcgtgac
     4261 gagccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaatatc
     4321 tattaaaact gataggtcat gccaaccgcg acaatatcat cattattaat a
//
LOCUS       DAMZII010000394         3374 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.258, whole genome shotgun sequence.
ACCESSION   DAMZII010000394 DAMZII010000000
VERSION     DAMZII010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.258"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            205..447
                     /locus_tag="POA77_005064"
     CDS             205..447
                     /locus_tag="POA77_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDI6368349.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(479..1129)
                     /gene="tetR(A)"
                     /locus_tag="POA77_005065"
     CDS             complement(479..1129)
                     /gene="tetR(A)"
                     /locus_tag="POA77_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDI6368350.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1235..2434
                     /gene="tet(A)"
                     /locus_tag="POA77_005066"
     CDS             1235..2434
                     /gene="tet(A)"
                     /locus_tag="POA77_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDI6368351.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2466..3326)
                     /locus_tag="POA77_005067"
     CDS             complement(2466..3326)
                     /locus_tag="POA77_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI6368352.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          641 a         1059 c         1051 g          623 t
ORIGIN      
        1 ggcgctggaa gccccgtagc gacgcggaga ggggcgagac aagccaaggg cgcaggctcg
       61 atgcgcagca cgacatagcc ggttctcgca aggacgagaa tttccctgcg gtgcccctca
      121 agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag ccttgagtcc acgctagatc
      181 tatctcatct gcgcaaggca gaacgtgaag acggccgccc tggacctcgc ccgcgagcgc
      241 caggcgcacg aggccggcgc gcggacccgc gccacggccc acgagcggac gccgcagcag
      301 gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag
      361 ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg
      421 gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc
      481 aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc
      541 catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg
      601 cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct
      661 cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga
      721 ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa
      781 gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg
      841 cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg
      901 agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt
      961 cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct
     1021 gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca
     1081 acaggtccag ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt
     1141 tatcactgat aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca
     1201 atcggaccag cggaggctgg tccggaggcc agacgtgaaa cccaacagac ccctgatcgt
     1261 aattctgagc actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc
     1321 gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct
     1381 ggcgctgtat gcgttgatgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg
     1441 tttcgggcgg cggccggtct tgctcgtctc gctggccggc gctgctgtcg actacgccat
     1501 catggcgacg gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac
     1561 cggggcgact ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg
     1621 cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt
     1681 gctcggtggg ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt
     1741 gaacggcctc aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg
     1801 ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat
     1861 gaccgtcgtc gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc
     1921 ggccgcgctt tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg
     1981 catttcgctt gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc
     2041 tgtagccgcc cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac
     2101 aggctacatc ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct
     2161 gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga
     2221 tgaggaacgt caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat
     2281 cgtcggaccc ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg
     2341 ggcatggatt gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct
     2401 ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg cctatgccat
     2461 gcgggtcaag gcgacttccg gcaagctata cgcgccctag gagtgcggtt ggaacgttgg
     2521 cccagccaga tactcccgat cacgagcagg acgccgatga tttgaagcgc actcagcgtc
     2581 tgatccaaga acaaccatcc tagcaacacg gcggtccccg ggctgagaaa gcccagtaag
     2641 gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc
     2701 gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt
     2761 ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag
     2821 gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac
     2881 gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt
     2941 ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc
     3001 aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca
     3061 gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc
     3121 cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt
     3181 ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg
     3241 accgtcatcg gtgagaagtt cggcaggtat tgggtggtga caatgtaggt gctgccccaa
     3301 atggcaggtg ctatcgctgt gaacaataaa tcggggcaga cggcgcttcc agcgtgacga
     3361 ccatgccgag cacg
//
LOCUS       DAMZII010000395         2858 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.272, whole genome shotgun sequence.
ACCESSION   DAMZII010000395 DAMZII010000000
VERSION     DAMZII010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.272"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            37..384
                     /locus_tag="POA77_005068"
     CDS             37..384
                     /locus_tag="POA77_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDI6368353.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            378..1217
                     /gene="sul1"
                     /locus_tag="POA77_005069"
     CDS             378..1217
                     /gene="sul1"
                     /locus_tag="POA77_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDI6368354.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1345..1845
                     /locus_tag="POA77_005070"
     CDS             1345..1845
                     /locus_tag="POA77_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6368355.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(2021..2803)
                     /gene="istB"
                     /locus_tag="POA77_005071"
     CDS             complement(2021..2803)
                     /gene="istB"
                     /locus_tag="POA77_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HDI6368356.1"
                     /translation="MQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSLI
                     KAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNVV
                     LIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLLY
                     ADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTTA
                     LLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
BASE COUNT          552 a          818 c          810 g          678 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
       61 atcgcaatag ttggcgaagt aatcgcaaca tccgcattaa aatctagcga gggctttact
      121 aagcttgccc cttccgccgt tgtcataatc ggttatggca tcgcatttta ttttctttct
      181 ctggttctga aatccatccc tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc
      241 gtcataatta cagccattgc ctggttgctt catgggcaaa agcttgatgc gtggggcttt
      301 gtaggtatgg ggctcataat tgctgccttt ttgctcgccc gatccccatc gtggaagtcg
      361 ctgcggaggc cgacgccatg gtgacggtgt tcggcattct gaatctcacc gaggactcct
      421 tcttcgatga gagccggcgg ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc
      481 tgcgagtcgg atcagacgtc gtggatgtcg gaccggccgc cagccatccg gacgcgaggc
      541 ctgtatcgcc ggccgatgag atcagacgta ttgcgccgct cttagacgcc ctgtccgatc
      601 agatgcaccg tgtttcaatc gacagcttcc aaccggaaac ccagcgctat gcgctcaagc
      661 gcggcgtggg ctacctgaac gatatccaag gatttcctga ccctgcgctc tatcccgata
      721 ttgctgaggc ggactgcagg ctggtggtta tgcactcagc gcagcgggat ggcatcgcca
      781 cccgcaccgg tcaccttcga cccgaagacg cgctcgacga gattgtgcgg ttcttcgagg
      841 cgcgggtttc cgccttgcga cggagcgggg tcgctgccga ccggctcatc ctcgatccgg
      901 ggatgggatt tttcttgagc cccgcaccgg aaacatcgct gcacgtgctg tcgaaccttc
      961 aaaagctgaa gtcggcgttg gggcttccgc tattggtctc ggtgtcgcgg aaatccttct
     1021 tgggcgccac cgttggcctt cctgtaaagg atctgggtcc agcgagcctt gcggcggaac
     1081 ttcacgcgat cggcaatggc gctgactacg tccgcaccca cgcgcctgga gatctgcgaa
     1141 gcgcaatcac cttctcggaa accctcgcga aatttcgcag tcgcgacgcc agagaccgag
     1201 ggttagatca tgcctagcat tcaccttccg gccgcccgct agcggaccct ggtcaggttc
     1261 cgcgaaggtg ggcgcagaca tgctgggctc gtcaggatca aactgcacta tgaggcggcg
     1321 gttcataccg cgccagggga gcgaatggac agcgaggagc ctccgaacgt tcgggtcgcc
     1381 tgctcgggtg atatcgacga ggttgtgcgg ctgatgcacg acgctgcggc gtggatgtcc
     1441 gccaagggaa cgcccgcctg ggacgtcgcg cggatcgacc ggacattcgc ggagaccttc
     1501 gtcctgagat ccgagctcct agtcgcgagt tgcagcgacg gcatcgtcgg ctgttgcacc
     1561 ttgtcggccg aggatcccga gttctggccc gacgccctca agggggaggc cgcatatctg
     1621 cacaagctcg cggtgcgacg gacacatgcg ggccggggtg tcagctccgc gctgatcgag
     1681 gcttgccgcc atgccgcgcg aacgcagggg tgcgccaagc tgcggctcga ctgccacccg
     1741 aacctgcgtg gcctatacga gcggctcgga ttcacccacg tcgacacttt caatcccggc
     1801 tgggatccaa ccttcatcgc agaacgccta gaactcgaaa tctaacgtcc gttcgggcat
     1861 cgaggtccat gtcggggtgg gacgggcccg tggcttcaag atcacttgca gtccgaccgc
     1921 gatgtcttgg ttgcgcgaga ggttgtcgat atctgttgat ttgcacccaa atttgacccg
     1981 ggatttgcat tgaattttga cccacccctt gttgtcagaa ttatgtctcg attttcagtt
     2041 tgcgggtctg tttttcctcc tgcttattct gagttgaact gtgtttgaag cggtaacttt
     2101 cattgccggt ttccaggatg tggcagtggt gggttagtcg gtccaacaac gctgttgtca
     2161 tcttttcatc gccaaacact cggctccatt ccgagaagct caagttggtg gtcagtatca
     2221 cgctggtttt ttcgtacagc tttgagagca ggtgaaacag cagtgcccca ccggtttggc
     2281 taaaaggcaa atatcccagc tcatccagaa tcaccaaatc ggcatacaac agacggtttg
     2341 cgatttgtcc ctgacgccca gatgatttct cttgctccag tgcattgacc aaatccacgg
     2401 tggagaagaa acgcacccgt cggttcaagt gcatcactgc ttgtgtacca atggctgtgg
     2461 ccaggtgagt cttgcctgtg cctggcccac caatcagcac cacgttctgg gcttgttcca
     2521 tgaagtcgca ccggtgcaat tgtttgaccg tggcctcatt aaccaggctt tgactgaagt
     2581 caaagcccac caagtcccga tacacgggga acttggccac ccgcaattga tagttcaccg
     2641 aacgtacttc acgctctgcc acttcagctt taatcaagct gtccagcatg ggcaaggctt
     2701 gattaaatgc tggtgaattc tgattgccca actcctcaat ggcgtgtgcc atgccaaaga
     2761 gtttcaagga tttgaggatt ctcacatggc cttcatgctg catcatgggc tctccttaaa
     2821 ctgtcatagc ggttcacgtt ggcctgtggt tccaatgt
//
LOCUS       DAMZII010000396         1884 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.302, whole genome shotgun sequence.
ACCESSION   DAMZII010000396 DAMZII010000000
VERSION     DAMZII010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.302"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="POA77_005072"
     CDS             40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="POA77_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDI6368357.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            1146..1466
                     /gene="groES"
                     /locus_tag="POA77_005073"
     CDS             1146..1466
                     /gene="groES"
                     /locus_tag="POA77_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6368358.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            1522..>1884
                     /gene="groEL"
                     /locus_tag="POA77_005074"
     CDS             1522..>1884
                     /gene="groEL"
                     /locus_tag="POA77_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6368359.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGID"
BASE COUNT          364 a          630 c          595 g          295 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga tgactgatcc ccgcaaaaac
       61 ggcgatttgc acgaacccgc gacggcaccc gcgacgccct ggtccaaaag cgagctggtc
      121 cggcaattgc gcgacctcgg cgtgcgctca ggcgatatgg tgatgccgca tgtgtcgttg
      181 cgcgccgtcg ggccgctggc ggacggaccg cagacacttg tcgatgcgct gatcgaggcc
      241 gtcggcccca ccgggaatat tctcgccttc gtctcgtggc gcgattcgcc ctatgaacag
      301 acgctgggtc atgatgcgcc gcccgccgcc atcgcccaaa gctggcctgc gttcgacccc
      361 gaccatgcgc ccgcctaccc cggctttggc gcgatcaacg aatttatccg aacctatccg
      421 gggtgtcggc gcagcgccca tcccgacgca tcgatggcgg cgatcgggcc cgatgcggcg
      481 tggctggtgg cgccgcacga gatgggcgcc gcttatggcc cccgctcgcc gatcgcgcgt
      541 tttctcgccc acgcaggaaa aatcctgtcg atcggcgccg ggcccgatgc agtcaccgcg
      601 ctccattatg ccgaagcggt ggcgcggatc gagggcaagc gccgcgtcac ttattcgatg
      661 cccttactgc gcgaaggcaa gcgcgtctgg gtcaccacgt ccgactggga ttcgaacggc
      721 atcctcgacg aatatgccgc gcccgacggc cccgacgcgg tcgaacggat cgcccgcgac
      781 tatctcgccc gcaccagggt tgcgcaaggc ccggtcggcg gcgcgcaatc ccggctgatc
      841 gacgcggccg atatcgtttc cttcggcatc gaatggctcg aggcgcgcca cgccgcgcca
      901 gcggcggcag cgctgaagcc gaaacaacgc cgcgactgag tcgtcgatta ttccggacat
      961 atattcctcc ctctcgccca gccgcatagc atcaggccca caagcccgcc gcctcttcaa
     1021 caaggatgtc gggacgttct tcagtaaaga acagcttggc accctcgaca aggatgattg
     1081 cccgaggcga tcaggcggcg aatttttgcg cgcgcgcacg gtcgcggcgg cgcgcgataa
     1141 aggcgatggg tagccccacg aggaagatat gcgcggcgat taacatcaag ccgctgtacg
     1201 accgcgtcgt catcaagcgc accgaggaag agaaggtctc cgccggtggt atcgtgatcc
     1261 cggattcggc caccgagaag ccgatcaagg gccaggtcgt cgccgtgggc gagggcaagg
     1321 tgttcgacaa cggcaacgtg cgcgcgccga aggtcaaggt cggcgaccag gtgctgttcg
     1381 gcaagtacgc cggcaccgag gtcaagctcg acggcaccga gtacctggtg gtgaaggaag
     1441 acgacatctt cgccgtgctg ggctgagccc gccgcttcgc cgcatcccaa cccgcaatca
     1501 cagaactgag gtaattccgc aatggctgcc aaggatattc gtttcggtga agacgcccgc
     1561 gcccgcatgg tccgcggcgt gaacgtgctc gccaacgccg tcaaggcgac cctgggcccg
     1621 aagggccgca acgtcgtgct cgagaagagc ttcggcgccc cgacgatcac caaggacggc
     1681 gtgtccgtcg ccaaggagat cgaactggcc gacaagttcg agaacatggg cgcgcagatg
     1741 gtcaaggaag tcgcttccaa gacctccgac aacgccggcg acggcaccac caccgccacc
     1801 gtgctggccc aggccctgat ccgcgagggc atgaaggccg tggccgccgg catgaacccg
     1861 atggacctca agcgcggtat cgac
//
LOCUS       DAMZII010000397          933 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNPP927
            SAMN32919432-rid16879373.guided.343, whole genome shotgun sequence.
ACCESSION   DAMZII010000397 DAMZII010000000
VERSION     DAMZII010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919432
            Sequence Read Archive: SRR23231951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591445.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591445.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:36:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,857
            CDSs (with protein)               :: 4,857
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 58 of 122
            Pseudo Genes (incomplete)         :: 59 of 122
            Pseudo Genes (internal stop)      :: 29 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919432-rid16879373.guided.343"
                     /strain="UMNPP927"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            48..839
                     /gene="aadA1"
                     /locus_tag="POA77_005075"
     CDS             48..839
                     /gene="aadA1"
                     /locus_tag="POA77_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDI6368360.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          238 a          235 c          253 g          207 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
       61 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      121 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
      181 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      241 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
      301 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
      361 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
      421 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
      481 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
      541 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
      601 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
      661 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
      721 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcc cgcgcagatc
      781 agttggaaga atttgttcac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
      841 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
      901 gcactaagca cataattgct cacagccaaa cta
//
